Antarctic and Sub-Antarctic Asteroidea database.

PubMed

Moreau, Camille; Mah, Christopher; Agüera, Antonio; Améziane, Nadia; David Barnes; Crokaert, Guillaume; Eléaume, Marc; Griffiths, Huw; Charlène Guillaumot; Hemery, Lenaïg G; Jażdżewska, Anna; Quentin Jossart; Vladimir Laptikhovsky; Linse, Katrin; Neill, Kate; Sands, Chester; Thomas Saucède; Schiaparelli, Stefano; Siciński, Jacek; Vasset, Noémie; Bruno Danis

2018-01-01

The present dataset is a compilation of georeferenced occurrences of asteroids (Echinodermata: Asteroidea) in the Southern Ocean. Occurrence data south of 45°S latitude were mined from various sources together with information regarding the taxonomy, the sampling source and sampling sites when available. Records from 1872 to 2016 were thoroughly checked to ensure the quality of a dataset that reaches a total of 13,840 occurrences from 4,580 unique sampling events. Information regarding the reproductive strategy (brooders vs. broadcasters) of 63 species is also made available. This dataset represents the most exhaustive occurrence database on Antarctic and Sub-Antarctic asteroids.

Evaluation of the NCPDP Structured and Codified Sig Format for e-prescriptions.

PubMed

Liu, Hangsheng; Burkhart, Q; Bell, Douglas S

2011-01-01

To evaluate the ability of the structure and code sets specified in the National Council for Prescription Drug Programs Structured and Codified Sig Format to represent ambulatory electronic prescriptions. We parsed the Sig strings from a sample of 20,161 de-identified ambulatory e-prescriptions into variables representing the fields of the Structured and Codified Sig Format. A stratified random sample of these representations was then reviewed by a group of experts. For codified Sig fields, we attempted to map the actual words used by prescribers to the equivalent terms in the designated terminology. Proportion of prescriptions that the Format could fully represent; proportion of terms used that could be mapped to the designated terminology. The fields defined in the Format could fully represent 95% of Sigs (95% CI 93% to 97%), but ambiguities were identified, particularly in representing multiple-step instructions. The terms used by prescribers could be codified for only 60% of dose delivery methods, 84% of dose forms, 82% of vehicles, 95% of routes, 70% of sites, 33% of administration timings, and 93% of indications. The findings are based on a retrospective sample of ambulatory prescriptions derived mostly from primary care physicians. The fields defined in the Format could represent most of the patient instructions in a large prescription sample, but prior to its mandatory adoption, further work is needed to ensure that potential ambiguities are addressed and that a complete set of terms is available for the codified fields.

The lunar quarantine program

NASA Technical Reports Server (NTRS)

Johnston, R. S.; Mason, J. A.; Wooley, B. C.; Mccollum, G. W.; Mieszkuc, B. J.

1974-01-01

The lunar quarantine program was designed to ensure that return of lunar material represented no threat to the public health, to agriculture, or to other living resources. It established definitely that no life exists on the moon. The crews of the three lunar quarantine missions, Apollo 11, 12, and 14, experienced no health problems as a result of their exposure to lunar samples. Plants and animals also showed no adverse effects. Stringent quarantine was terminated after Apollo 14, but lunar samples continued to be protected to guarantee that scientists would receive uncontaminated materials for study.

Proximate Composition Analysis.

PubMed

2016-01-01

The proximate composition of foods includes moisture, ash, lipid, protein and carbohydrate contents. These food components may be of interest in the food industry for product development, quality control (QC) or regulatory purposes. Analyses used may be rapid methods for QC or more accurate but time-consuming official methods. Sample collection and preparation must be considered carefully to ensure analysis of a homogeneous and representative sample, and to obtain accurate results. Estimation methods of moisture content, ash value, crude lipid, total carbohydrates, starch, total free amino acids and total proteins are put together in a lucid manner.

Naval Sea Systems Command Did Not Properly Apply Guidance Regarding Contracting Officers Representatives

DTIC Science & Technology

2016-03-18

Contract _______________________________ 10 PCO and ACO Did Not Ensure Appointment of COR ________________________________________ 12 NAVSEA Did Not Properly...sample was selected, NAVSEA had issued work for 36 availabilities on the contract—obligating $102.5M. { The procuring contracting officer ( PCO ) at...Responsibilities,” Section 1.602-2, “Responsibilities,” May 29, 2014. 3 The contracting officers for the contract reviewed are the PCO at NAVSEA

[Biosafety in the study of samples of extraterrestrial origin by an example of preparation for the "Phobos-Grunt" mission].

PubMed

Dobrokhotskiĭ, O N; Diatlov, I A; Orlov, O I; Novikova, N D; Khamidullina, N M; Deshevaia, E A

2012-01-01

The necessity of microbial studying the soil from Phobos and terrestrial biological objects, which were for a long time in deep space and may represent a biological hazard has been shown. Developed medical and technical requirements for laboratories comply with Russian regulations and recommendations of international instruments for the ensuring of biosafety on the basis of process (continuous) biorisk management.

Evaluation of the NCPDP Structured and Codified Sig Format for e-prescriptions

PubMed Central

Burkhart, Q; Bell, Douglas S

2011-01-01

Objective To evaluate the ability of the structure and code sets specified in the National Council for Prescription Drug Programs Structured and Codified Sig Format to represent ambulatory electronic prescriptions. Design We parsed the Sig strings from a sample of 20 161 de-identified ambulatory e-prescriptions into variables representing the fields of the Structured and Codified Sig Format. A stratified random sample of these representations was then reviewed by a group of experts. For codified Sig fields, we attempted to map the actual words used by prescribers to the equivalent terms in the designated terminology. Measurements Proportion of prescriptions that the Format could fully represent; proportion of terms used that could be mapped to the designated terminology. Results The fields defined in the Format could fully represent 95% of Sigs (95% CI 93% to 97%), but ambiguities were identified, particularly in representing multiple-step instructions. The terms used by prescribers could be codified for only 60% of dose delivery methods, 84% of dose forms, 82% of vehicles, 95% of routes, 70% of sites, 33% of administration timings, and 93% of indications. Limitations The findings are based on a retrospective sample of ambulatory prescriptions derived mostly from primary care physicians. Conclusion The fields defined in the Format could represent most of the patient instructions in a large prescription sample, but prior to its mandatory adoption, further work is needed to ensure that potential ambiguities are addressed and that a complete set of terms is available for the codified fields. PMID:21613642

The Chandra Strong Lens Sample: Revealing Baryonic Physics In Strong Lensing Selected Clusters

NASA Astrophysics Data System (ADS)

Bayliss, Matthew

2017-08-01

We propose for Chandra imaging of the hot intra-cluster gas in a unique new sample of 29 galaxy clusters selected purely on their strong gravitational lensing signatures. This will be the first program targeting a purely strong lensing selected cluster sample, enabling new comparisons between the ICM properties and scaling relations of strong lensing and mass/ICM selected cluster samples. Chandra imaging, combined with high precision strong lens models, ensures powerful constraints on the distribution and state of matter in the cluster cores. This represents a novel angle from which we can address the role played by baryonic physics |*| the infamous |*|gastrophysics|*| in shaping the cores of massive clusters, and opens up an exciting new galaxy cluster discovery space with Chandra.

The Chandra Strong Lens Sample: Revealing Baryonic Physics In Strong Lensing Selected Clusters

NASA Astrophysics Data System (ADS)

Bayliss, Matthew

2017-09-01

We propose for Chandra imaging of the hot intra-cluster gas in a unique new sample of 29 galaxy clusters selected purely on their strong gravitational lensing signatures. This will be the first program targeting a purely strong lensing selected cluster sample, enabling new comparisons between the ICM properties and scaling relations of strong lensing and mass/ICM selected cluster samples. Chandra imaging, combined with high precision strong lens models, ensures powerful constraints on the distribution and state of matter in the cluster cores. This represents a novel angle from which we can address the role played by baryonic physics -- the infamous ``gastrophysics''-- in shaping the cores of massive clusters, and opens up an exciting new galaxy cluster discovery space with Chandra.

Cancer Liquid Biopsy: Is It Ready for Clinic?

PubMed

Pan, Ying; Ji, John S; Jin, Jason Gang; Kuo, Winston Patrick; Kang, Hongjun

2017-01-01

The management of cancer relies on a combination of imaging and tissue biopsy for diagnosis, monitoring, and molecular classification-based patient stratification to ensure appropriate treatment. Conventional tissue biopsy harvests tumor samples with invasive procedures, which are often difficult for patients with advanced disease. Given the well-recognized intratumor genetic heterogeneity [1], the biopsy of small tumor fragments does not necessarily represent all the genetic aberrations in the tumor, but sampling the entire tumor in each patient is not realistic. Moreover, tumors evolve all the time from local to advanced disease and by adapting to selective pressure from treatment.

Methods for Investigating Mercury Speciation, Transport, Methylation, and Bioaccumulation in Watersheds Affected by Historical Mining

NASA Astrophysics Data System (ADS)

Alpers, C. N.; Marvin-DiPasquale, M. C.; Fleck, J.; Ackerman, J. T.; Eagles-Smith, C.; Stewart, A. R.; Windham-Myers, L.

2016-12-01

Many watersheds in the western U.S. have mercury (Hg) contamination from historical mining of Hg and precious metals (gold and silver), which were concentrated using Hg amalgamation (mid 1800's to early 1900's). Today, specialized sampling and analytical protocols for characterizing Hg and methylmercury (MeHg) in water, sediment, and biota generate high-quality data to inform management of land, water, and biological resources. Collection of vertically and horizontally integrated water samples in flowing streams and use of a Teflon churn splitter or cone splitter ensure that samples and subsamples are representative. Both dissolved and particulate components of Hg species in water are quantified because each responds to different hydrobiogeochemical processes. Suspended particles trapped on pre-combusted (Hg-free) glass- or quartz-fiber filters are analyzed for total mercury (THg), MeHg, and reactive divalent mercury. Filtrates are analyzed for THg and MeHg to approximate the dissolved fraction. The sum of concentrations in particulate and filtrate fractions represents whole water, equivalent to an unfiltered sample. This approach improves upon analysis of filtered and unfiltered samples and computation of particulate concentration by difference; volume filtered is adjusted based on suspended-sediment concentration to minimize particulate non-detects. Information from bed-sediment sampling is enhanced by sieving into multiple size fractions and determining detailed grain-size distribution. Wet sieving ensures particle disaggregation; sieve water is retained and fines are recovered by centrifugation. Speciation analysis by sequential extraction and examination of heavy mineral concentrates by scanning electron microscopy provide additional information regarding Hg mineralogy and geochemistry. Biomagnification of MeHg in food webs is tracked using phytoplankton, zooplankton, aquatic and emergent vegetation, invertebrates, fish, and birds. Analysis of zooplankton in multiple size fractions from multiple depths in reservoirs can provide insight into food-web dynamics. The presentation will highlight application of these methods in several Hg-contaminated watersheds, with emphasis on understanding seasonal variability in designing effective sampling strategies.

Low Pt content direct methanol fuel cell anode catalyst: nanophase PtRuNiZr

NASA Technical Reports Server (NTRS)

Whitacre, Jay F. (Inventor); Narayanan, Sekharipuram R. (Inventor)

2010-01-01

A method for the preparation of a metallic material having catalytic activity that includes synthesizing a material composition comprising a metal content with a lower Pt content than a binary alloy containing Pt but that displays at least a comparable catalytic activity on a per mole Pt basis as the binary alloy containing Pt; and evaluating a representative sample of the material composition to ensure that the material composition displays a property of at least a comparable catalytic activity on a per mole Pt basis as a representative binary alloy containing Pt. Furthermore, metallic compositions are disclosed that possess substantial resistance to corrosive acids.

Comparing adult cannabis treatment-seekers enrolled in a clinical trial with national samples of cannabis users in the United States

PubMed Central

McClure, Erin A.; King, Jacqueline S.; Wahle, Aimee; Matthews, Abigail G.; Sonne, Susan C.; Lofwall, Michelle R.; McRae-Clark, Aimee L.; Ghitza, Udi E.; Martinez, Melissa; Cloud, Kasie; Virk, Harvir S.; Gray, Kevin M.

2017-01-01

Background Cannabis use rates are increasing among adults in the United States (US) while the perception of harm is declining. This may result in an increased prevalence of cannabis use disorder and the need for more clinical trials to evaluate efficacious treatment strategies. Clinical trials are the gold standard for evaluating treatment, yet study samples are rarely representative of the target population. This finding has not yet been established for cannabis treatment trials. This study compared demographic and cannabis use characteristics of a cannabis cessation clinical trial sample (run through National Drug Abuse Treatment Clinical Trials Network) with three nationally representative datasets from the US; 1) National Survey on Drug Use and Health, 2) National Epidemiologic Survey on Alcohol and Related Conditions-III, and 3) Treatment Episodes Data Set – Admissions. Methods Comparisons were made between the clinical trial sample and appropriate cannabis using sub-samples from the national datasets, and propensity scores were calculated to determine the degree of similarity between samples. Results Results showed that the clinical trial sample was significantly different from all three national datasets, with the clinical trial sample having greater representation among older adults, African Americans, Hispanic/Latinos, adults with more education, non-tobacco users, and daily and almost daily cannabis users. Conclusions These results are consistent with previous studies of other substance use disorder populations and extend sample representation issues to a cannabis use disorder population. This illustrates the need to ensure representative samples within cannabis treatment clinical trials to improve the generalizability of promising findings. PMID:28511033

Comparing adult cannabis treatment-seekers enrolled in a clinical trial with national samples of cannabis users in the United States.

PubMed

McClure, Erin A; King, Jacqueline S; Wahle, Aimee; Matthews, Abigail G; Sonne, Susan C; Lofwall, Michelle R; McRae-Clark, Aimee L; Ghitza, Udi E; Martinez, Melissa; Cloud, Kasie; Virk, Harvir S; Gray, Kevin M

2017-07-01

Cannabis use rates are increasing among adults in the United States (US) while the perception of harm is declining. This may result in an increased prevalence of cannabis use disorder and the need for more clinical trials to evaluate efficacious treatment strategies. Clinical trials are the gold standard for evaluating treatment, yet study samples are rarely representative of the target population. This finding has not yet been established for cannabis treatment trials. This study compared demographic and cannabis use characteristics of a cannabis cessation clinical trial sample (run through National Drug Abuse Treatment Clinical Trials Network) with three nationally representative datasets from the US; 1) National Survey on Drug Use and Health, 2) National Epidemiologic Survey on Alcohol and Related Conditions-III, and 3) Treatment: Episodes Data Set - Admissions. Comparisons were made between the clinical trial sample and appropriate cannabis using sub-samples from the national datasets, and propensity scores were calculated to determine the degree of similarity between samples. showed that the clinical trial sample was significantly different from all three national datasets, with the clinical trial sample having greater representation among older adults, African Americans, Hispanic/Latinos, adults with more education, non-tobacco users, and daily and almost daily cannabis users. These results are consistent with previous studies of other substance use disorder populations and extend sample representation issues to a cannabis use disorder population. This illustrates the need to ensure representative samples within cannabis treatment clinical trials to improve the generalizability of promising findings. Copyright © 2017 Elsevier B.V. All rights reserved.

Re-evaluation of Moisture Controls During ARIES Oxide Processing, Packaging and Characterization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karmiol, Benjamin; Wayne, David Matthew

DOE-STD-3013 [1] requires limiting the relative humidity (RH) in the glovebox during processing of the oxide product for specific types of plutonium oxides. This requirement is mandated in order to limit corrosion of the stainless steel containers by deliquescence of chloride salts if present in the PuO2. DOE-STD-3013 also specifies the need to limit and monitor internal pressure buildup in the 3013 containers due to the potential for the generation of free H2 and O2 gas from the radiolysis of surfaceadsorbed water. DOE-STD-3013 requires that the oxide sample taken for moisture content verification be representative of the stabilized material inmore » the 3013 container. This is accomplished by either limiting the time between sampling and packaging, or by control of the glovebox relative humidity (%RH). This requirement ensures that the sample is not only representative, but also conservative from the standpoint of moisture content.« less

Image Discrimination Models With Stochastic Channel Selection

NASA Technical Reports Server (NTRS)

Ahumada, Albert J., Jr.; Beard, Bettina L.; Null, Cynthia H. (Technical Monitor)

1995-01-01

Many models of human image processing feature a large fixed number of channels representing cortical units varying in spatial position (visual field direction and eccentricity) and spatial frequency (radial frequency and orientation). The values of these parameters are usually sampled at fixed values selected to ensure adequate overlap considering the bandwidth and/or spread parameters, which are usually fixed. Even high levels of overlap does not always ensure that the performance of the model will vary smoothly with image translation or scale changes. Physiological measurements of bandwidth and/or spread parameters result in a broad distribution of estimated parameter values and the prediction of some psychophysical results are facilitated by the assumption that these parameters also take on a range of values. Selecting a sample of channels from a continuum of channels rather than using a fixed set can make model performance vary smoothly with changes in image position, scale, and orientation. It also facilitates the addition of spatial inhomogeneity, nonlinear feature channels, and focus of attention to channel models.

Screening Cereals Quality by Electronic Nose: the Example of Mycotoxins Naturally Contaminated Maize and Durum Wheat

NASA Astrophysics Data System (ADS)

Campagnoli, Anna; Dell'Orto, Vittorio; Savoini, Giovanni; Cheli, Federica

2009-05-01

Mycotoxins represent an heterogeneous group of toxic compounds from fungi metabolism. Due to the frequent occurrence of mycotoxins in cereals commodities the develop of cost/effective screening methods represent an important topic to ensure food and feed safety. In the presented study a commercial electronic nose constituted by ten MOS (Metal Oxide Sensors) was applied to verify the possibility of discriminating between mycotoxins contaminated and non-contaminated cereals. The described analytical approach was able to discriminate contaminated and non-contaminated samples both in the case of aflatoxins infected maize and deoxynivalenol infected durum wheat samples. In the case of maize data two sensors from the array revealed a partial relation with the level of aflatoxins. These results could be promising for a further improvement of electronic nose application in order to develop a semi-quantitative screening approach to mycotoxins contamination.

Sampling maternal care behaviour in domestic dogs: What's the best approach?

PubMed

Czerwinski, Veronika H; Smith, Bradley P; Hynd, Philip I; Hazel, Susan J

2017-07-01

Our understanding of the frequency and duration of maternal care behaviours in the domestic dog during the first two postnatal weeks is limited, largely due to the inconsistencies in the sampling methodologies that have been employed. In order to develop a more concise picture of maternal care behaviour during this period, and to help establish the sampling method that represents these behaviours best, we compared a variety of time sampling methods Six litters were continuously observed for a total of 96h over postnatal days 3, 6, 9 and 12 (24h per day). Frequent (dam presence, nursing duration, contact duration) and infrequent maternal behaviours (anogenital licking duration and frequency) were coded using five different time sampling methods that included: 12-h night (1800-0600h), 12-h day (0600-1800h), one hour period during the night (1800-0600h), one hour period during the day (0600-1800h) and a one hour period anytime. Each of the one hour time sampling method consisted of four randomly chosen 15-min periods. Two random sets of four 15-min period were also analysed to ensure reliability. We then determined which of the time sampling methods averaged over the three 24-h periods best represented the frequency and duration of behaviours. As might be expected, frequently occurring behaviours were adequately represented by short (oneh) sampling periods, however this was not the case with the infrequent behaviour. Thus, we argue that the time sampling methodology employed must match the behaviour of interest. This caution applies to maternal behaviour in altricial species, such as canids, as well as all systematic behavioural observations utilising time sampling methodology. Copyright © 2017. Published by Elsevier B.V.

Listeria spp. in Street-Vended Ready-to-Eat Foods

PubMed Central

El-Shenawy, Moustafa; El-Shenawy, Mohamed; Mañes, Jordi; Soriano, Jose M.

2011-01-01

Street-vended ready-to-eat food sold in Egypt, including sandwiches and dishes of traditional food, was examined for the presence of Listeria species. Out of 576 samples, 24% were found to contain Listeria species. L. monocytogenes and L. innocua were isolated from 57% and 39% of the contaminated samples, respectively. Other Listeria spp. were detected with lower frequency. L. monocytogenes of ≥103 CFU/g were detected in 7% of the total examined samples, which represent 49% of the contaminated food samples (meat, poultry, seafood, dairy products, and products of plant origin). Most of the samples contaminated by L. monocytogenes had high levels of total viable bacterial counts. The results obtained may help to clarify the epidemiology of listeriosis in the country and draw the attention of the decision makers to issue hygienic regulations for food processing industries as well as street vendors in order to ensure safe street-vended ready-to-eat food. PMID:22194742

[Comprehensive sexual education in Mexico: an analysis of coverage, comprehensiveness and continuity of contents in Mexican public and private schools].

PubMed

Rojas, Rosalba; Castro, Filipa de; Villalobos, Aremis; Allen-Leigh, Betania; Romero, Martin; Braverman-Bronstein, Ariela; Uribe, Patricia

2017-01-01

To analyze coverage of comprehensive sex education (CSE) in high schools in Mexico and describe whether it is comprehensive, homogeneous and has continuity based on student reports of exposure to topics in three dimensions: reproductive and sexual health, self-efficacy and rights and relations. Within a probabilistic, cross-sectional survey with stratified, cluster sampling, a nationally representative sample of 3 824 adolescents attending 45 public and private high-schools in urban and rural areas completed questionnaires on CSE. The proportion of adolescents reporting having received sex education from school personnel varies depending on topics and grade level. Topics most frequently covered are those related to sexual and reproductive health while rights and relations are least frequently dealt with. Most sex education topics are covered during junior high school and much less frequently in elementary or high school. CSE needs to be comprehensive and homogenous in terms of content, ensure inclusion of priority topics, meet national and international recommendations, ensure continuity and adapt contents to student age through all education levels.

Evaluation of the US Army Institute of Public Health Destination Monitoring Program, a food safety surveillance program.

PubMed

Rapp-Santos, Kamala; Havas, Karyn; Vest, Kelly

2015-01-01

The Destination Monitoring Program, operated by the US Army Public Health Command (APHC), is one component that supports the APHC Veterinary Service's mission to ensure safety and quality of food procured for the Department of Defense (DoD). This program relies on retail product testing to ensure compliance of production facilities and distributors that supply food to the DoD. This program was assessed to determine the validity and timeliness by specifically evaluating whether sample size of items collected was adequate, if food samples collected were representative of risk, and whether the program returns results in a timely manner. Data was collected from the US Army Veterinary Services Lotus Notes database, including all food samples collected and submitted from APHC Region-North for the purposes of destination monitoring from January 1, 2013 to December 31, 2013. For most food items, only one sample was submitted for testing. The ability to correctly identify a contaminated food lot may be limited by reliance on test results from only one sample, as the level of confidence in a negative test result is low. The food groups most frequently sampled by APHC correlated with the commodities that were implicated in foodborne illness in the United States. Food items to be submitted were equally distributed among districts and branches, but sections within large branches submitted relatively few food samples compared to sections within smaller branches and districts. Finally, laboratory results were not available for about half the food items prior to their respective expiration dates.

Datasets for Ostrava PMF paper

EPA Pesticide Factsheets

These data support a published journal paper described as follows:A 14-week investigation during a warm and cold seasons was conducted to improve understanding of airpollution sources that might be impacting air quality in Ostrava, the Czech Republic. Fine particulatematter (PM2.5) samples were collected in consecutive 12-h day and night increments during spring andfall 2012 sampling campaigns. Sampling sites were strategically located to evaluate conditions in closeproximity of a large steel works industrial complex, as well as away from direct influence of theindustrial complex. These samples were analyzed for metals and other elements, organic and elemental(black) carbon, and polycyclic aromatic hydrocarbons (PAHs). The PM2.5 samples were supplementedwith pollutant gases and meteorological parameters. We applied the EPA PMF v5.1 model with uncertainty estimate features to the Ostrava data set. Using the model's bootstrapping procedure and other considerations, six factors were determined to provide the optimum solution. Each model run consisted of 100 iterations to ensure that the solution represents a global minimum. The resulting factors were identified as representing coal (power plants), mixed Cl, crustal, industrial 1 (alkali metals and PAHs), industrial 2 (transition metals), and home heat/transportation. The home heating source is thought to be largely domestic boilers burning low quality fuels such as lignite, wood, and domestic waste.Transportation-r

Physical Activity of German Children and Adolescents 2003–2012: The MoMo-Study

PubMed Central

Henn, Annette; Albrecht, Claudia; Woll, Alexander

2017-01-01

Organized and unorganized physical activity (PA) hold an important role in the daily life of children and adolescents. Regular representative tracking of PA in different settings is important to evaluate social trends and implemented interventions. In this paper, representative PA data of German children and adolescents from the MoMo Baseline-Study (2004, n = 4528) are compared to those of Wave 1 (2010, n = 3994). Participants aged 4–17 were drawn out of 167 sample points in Germany and the data was weighted to ensure representativeness. PA was measured via questionnaire and was differentiated between organized (sports clubs and schools) and unorganized (unorganized sports and playing outside). Organized PA in extracurricular activities and sports clubs increased by eight percent, while unorganized PA decreased by seven percent. In addition to sports clubs, schools became a more prevalent setting for participation in physical activity in Germany. PMID:29137127

Physical Activity of German Children and Adolescents 2003-2012: The MoMo-Study.

PubMed

Schmidt, Steffen C E; Henn, Annette; Albrecht, Claudia; Woll, Alexander

2017-11-11

Organized and unorganized physical activity (PA) hold an important role in the daily life of children and adolescents. Regular representative tracking of PA in different settings is important to evaluate social trends and implemented interventions. In this paper, representative PA data of German children and adolescents from the MoMo Baseline-Study (2004, n = 4528) are compared to those of Wave 1 (2010, n = 3994). Participants aged 4-17 were drawn out of 167 sample points in Germany and the data was weighted to ensure representativeness. PA was measured via questionnaire and was differentiated between organized (sports clubs and schools) and unorganized (unorganized sports and playing outside). Organized PA in extracurricular activities and sports clubs increased by eight percent, while unorganized PA decreased by seven percent. In addition to sports clubs, schools became a more prevalent setting for participation in physical activity in Germany.

Building an Internet of Samples: The Australian Contribution

NASA Astrophysics Data System (ADS)

Wyborn, Lesley; Klump, Jens; Bastrakova, Irina; Devaraju, Anusuriya; McInnes, Brent; Cox, Simon; Karssies, Linda; Martin, Julia; Ross, Shawn; Morrissey, John; Fraser, Ryan

2017-04-01

Physical samples are often the ground truth to research reported in the scientific literature across multiple domains. They are collected by many different entities (individual researchers, laboratories, government agencies, mining companies, citizens, museums, etc.). Samples must be curated over the long-term to ensure both that their existence is known, and to allow any data derived from them through laboratory and field tests to be linked to the physical samples. For example, having unique identifiers that link back ground truth data on the original sample helps calibrate large volumes of remotely sensed data. Access to catalogues of reliably identified samples from several collections promotes collaboration across all Earth Science disciplines. It also increases the cost effectiveness of research by reducing the need to re-collect samples in the field. The assignment of web identifiers to the digital representations of these physical objects allows us to link to data, literature, investigators and institutions, thus creating an "Internet of Samples". An Australian implementation of the "Internet of Samples" is using the IGSN (International Geo Sample Number, http://igsn.github.io) to identify samples in a globally unique and persistent way. IGSN was developed in the solid earth science community and is recommended for sample identification by the Coalition for Publishing Data in the Earth and Space Sciences (COPDESS). IGSN is interoperable with other persistent identifier systems such as DataCite. Furthermore, the basic IGSN description metadata schema is compatible with existing schemas such as OGC Observations and Measurements (O&M) and DataCite Metadata Schema which makes crosswalks to other metadata schemas easy. IGSN metadata is disseminated through the Open Archives Initiative Protocol for Metadata Harvesting (OAI-PMH) allowing it to be aggregated in other applications such as portals (e.g. the Australian IGSN catalogue http://igsn2.csiro.au). The metadata is available in more than one format. The software for IGSN web services is based on components developed for DataCite and adapted to the specific requirements of IGSN. This cooperation in open source development ensures sustainable implementation and faster turnaround times for updates. IGSN, in particular in its Australian implementation, is characterised by a federated approach to system architecture and organisational governance giving it the necessary flexibility to adapt to particular local practices within multiple domains, whilst maintaining an overarching international standard. The three current IGSN allocation agents in Australia: Geoscience Australia, CSIRO and Curtin University, represent different sectors. Through funding from the Australian Research Data Services Program they have combined to develop a common web portal that allows discovery of physical samples and sample collections at a national level.International governance then ensures we can link to an international community but at the same time act locally to ensure the services offered are relevant to the needs of Australian researchers. This flexibility aids the integration of new disciplines into a global community of a physical samples information network.

Automated liver sampling using a gradient dual-echo Dixon-based technique.

PubMed

Bashir, Mustafa R; Dale, Brian M; Merkle, Elmar M; Boll, Daniel T

2012-05-01

Magnetic resonance spectroscopy of the liver requires input from a physicist or physician at the time of acquisition to insure proper voxel selection, while in multiecho chemical shift imaging, numerous regions of interest must be manually selected in order to ensure analysis of a representative portion of the liver parenchyma. A fully automated technique could improve workflow by selecting representative portions of the liver prior to human analysis. Complete volumes from three-dimensional gradient dual-echo acquisitions with two-point Dixon reconstruction acquired at 1.5 and 3 T were analyzed in 100 subjects, using an automated liver sampling algorithm, based on ratio pairs calculated from signal intensity image data as fat-only/water-only and log(in-phase/opposed-phase) on a voxel-by-voxel basis. Using different gridding variations of the algorithm, the average correct liver volume samples ranged from 527 to 733 mL. The average percentage of sample located within the liver ranged from 95.4 to 97.1%, whereas the average incorrect volume selected was 16.5-35.4 mL (2.9-4.6%). Average run time was 19.7-79.0 s. The algorithm consistently selected large samples of the hepatic parenchyma with small amounts of erroneous extrahepatic sampling, and run times were feasible for execution on an MRI system console during exam acquisition. Copyright © 2011 Wiley Periodicals, Inc.

Lipidomic analysis of biological samples: Comparison of liquid chromatography, supercritical fluid chromatography and direct infusion mass spectrometry methods.

PubMed

Lísa, Miroslav; Cífková, Eva; Khalikova, Maria; Ovčačíková, Magdaléna; Holčapek, Michal

2017-11-24

Lipidomic analysis of biological samples in a clinical research represents challenging task for analytical methods given by the large number of samples and their extreme complexity. In this work, we compare direct infusion (DI) and chromatography - mass spectrometry (MS) lipidomic approaches represented by three analytical methods in terms of comprehensiveness, sample throughput, and validation results for the lipidomic analysis of biological samples represented by tumor tissue, surrounding normal tissue, plasma, and erythrocytes of kidney cancer patients. Methods are compared in one laboratory using the identical analytical protocol to ensure comparable conditions. Ultrahigh-performance liquid chromatography/MS (UHPLC/MS) method in hydrophilic interaction liquid chromatography mode and DI-MS method are used for this comparison as the most widely used methods for the lipidomic analysis together with ultrahigh-performance supercritical fluid chromatography/MS (UHPSFC/MS) method showing promising results in metabolomics analyses. The nontargeted analysis of pooled samples is performed using all tested methods and 610 lipid species within 23 lipid classes are identified. DI method provides the most comprehensive results due to identification of some polar lipid classes, which are not identified by UHPLC and UHPSFC methods. On the other hand, UHPSFC method provides an excellent sensitivity for less polar lipid classes and the highest sample throughput within 10min method time. The sample consumption of DI method is 125 times higher than for other methods, while only 40μL of organic solvent is used for one sample analysis compared to 3.5mL and 4.9mL in case of UHPLC and UHPSFC methods, respectively. Methods are validated for the quantitative lipidomic analysis of plasma samples with one internal standard for each lipid class. Results show applicability of all tested methods for the lipidomic analysis of biological samples depending on the analysis requirements. Copyright © 2017 Elsevier B.V. All rights reserved.

Sampling Methods in Cardiovascular Nursing Research: An Overview.

PubMed

Kandola, Damanpreet; Banner, Davina; O'Keefe-McCarthy, Sheila; Jassal, Debbie

2014-01-01

Cardiovascular nursing research covers a wide array of topics from health services to psychosocial patient experiences. The selection of specific participant samples is an important part of the research design and process. The sampling strategy employed is of utmost importance to ensure that a representative sample of participants is chosen. There are two main categories of sampling methods: probability and non-probability. Probability sampling is the random selection of elements from the population, where each element of the population has an equal and independent chance of being included in the sample. There are five main types of probability sampling including simple random sampling, systematic sampling, stratified sampling, cluster sampling, and multi-stage sampling. Non-probability sampling methods are those in which elements are chosen through non-random methods for inclusion into the research study and include convenience sampling, purposive sampling, and snowball sampling. Each approach offers distinct advantages and disadvantages and must be considered critically. In this research column, we provide an introduction to these key sampling techniques and draw on examples from the cardiovascular research. Understanding the differences in sampling techniques may aid nurses in effective appraisal of research literature and provide a reference pointfor nurses who engage in cardiovascular research.

Total Diet Studies as a Tool for Ensuring Food Safety

PubMed Central

Lee, Joon-Goo; Kim, Sheen-Hee; Kim, Hae-Jung

2015-01-01

With the diversification and internationalization of the food industry and the increased focus on health from a majority of consumers, food safety policies are being implemented based on scientific evidence. Risk analysis represents the most useful scientific approach for making food safety decisions. Total diet study (TDS) is often used as a risk assessment tool to evaluate exposure to hazardous elements. Many countries perform TDSs to screen for chemicals in foods and analyze exposure trends to hazardous elements. TDSs differ from traditional food monitoring in two major aspects: chemicals are analyzed in food in the form in which it will be consumed and it is cost-effective in analyzing composite samples after processing multiple ingredients together. In Korea, TDSs have been conducted to estimate dietary intakes of heavy metals, pesticides, mycotoxins, persistent organic pollutants, and processing contaminants. TDSs need to be carried out periodically to ensure food safety. PMID:26483881

Recommendations for clinical biomarker specimen preservation and stability assessments.

PubMed

Dakappagari, Naveen; Zhang, Hui; Stephen, Laurie; Amaravadi, Lakshmi; Khan, Masood U

2017-04-01

With the wide use of biomarkers to enable critical drug-development decisions, there is a growing concern from scientific community on the need for a 'standardized process' for ensuring biomarker specimen stability and hence, a strong desire to share best practices on preserving the integrity of biomarker specimens in clinical trials and the design of studies to evaluate analyte stability. By leveraging representative industry experience, we have attempted to provide an overview of critical aspects of biomarker specimen stability commonly encountered during clinical development, including: planning of clinical sample collection procedures, clinical site training, selection of sample preservation buffers, shipping logistics, fit-for-purpose stability assessments in the analytical laboratory and presentation of case studies covering widely utilized biomarker specimen types.

Data Stewardship in the Ocean Sciences Needs to Include Physical Samples

NASA Astrophysics Data System (ADS)

Carter, M.; Lehnert, K.

2016-02-01

Across the Ocean Sciences, research involves the collection and study of samples collected above, at, and below the seafloor, including but not limited to rocks, sediments, fluids, gases, and living organisms. Many domains in the Earth Sciences have recently expressed the need for better discovery, access, and sharing of scientific samples and collections (EarthCube End-User Domain workshops, 2012 and 2013, http://earthcube.org/info/about/end-user-workshops), as has the US government (OSTP Memo, March 2014). iSamples (Internet of Samples in the Earth Sciences) is a Research Coordination Network within the EarthCube program that aims to advance the use of innovative cyberinfrastructure to support and advance the utility of physical samples and sample collections for science and ensure reproducibility of sample-based data and research results. iSamples strives to build, grow, and foster a new community of practice, in which domain scientists, curators of sample repositories and collections, computer and information scientists, software developers and technology innovators engage in and collaborate on defining, articulating, and addressing the needs and challenges of physical samples as a critical component of digital data infrastructure. A primary goal of iSamples is to deliver a community-endorsed set of best practices and standards for the registration, description, identification, and citation of physical specimens and define an actionable plan for implementation. iSamples conducted a broad community survey about sample sharing and has created 5 different working groups to address the different challenges of developing the internet of samples - from metadata schemas and unique identifiers to an architecture for a shared cyberinfrastructure to manage collections, to digitization of existing collections, to education, and ultimately to establishing the physical infrastructure that will ensure preservation and access of the physical samples. Repositories that curate marine sediment cores and dredge samples from the oceanic crust are participating in iSamples, but many other samples collected in the Ocean sciences are not yet represented. This presentation aims to engage a wider spectrum of Ocean scientists and sample curators in iSamples.

Hardness of AISI type 410 martensitic steels after high temperature irradiation via nanoindentation

NASA Astrophysics Data System (ADS)

Waseem, Owais Ahmed; Jeong, Jong-Ryul; Park, Byong-Guk; Maeng, Cheol-Soo; Lee, Myoung-Goo; Ryu, Ho Jin

2017-11-01

The hardness of irradiated AISI type 410 martensitic steel, which is utilized in structural and magnetic components of nuclear power plants, is investigated in this study. Proton irradiation of AISI type 410 martensitic steel samples was carried out by exposing the samples to 3 MeV protons up to a 1.0 × 1017 p/cm2 fluence level at a representative nuclear reactor coolant temperature of 350 °C. The assessment of deleterious effects of irradiation on the micro-structure and mechanical behavior of the AISI type 410 martensitic steel samples via transmission electron microscopy-energy dispersive spectroscopy and cross-sectional nano-indentation showed no significant variation in the microscopic or mechanical characteristics. These results ensure the integrity of the structural and magnetic components of nuclear reactors made of AISI type 410 martensitic steel under high-temperature irradiation damage levels up to approximately 5.2 × 10-3 dpa.

Demythologizing sex education in Oklahoma: an attitudinal study.

PubMed

Turner, N H

1983-08-01

A randomized study was conducted to determine the distribution of attitudes among Oklahomans of voting age toward sex education and to analyze the relationship of demographic, sociocultural, and attitudinal factors. The state was stratified into six regions. Forty-five percent of the sample lived in urban areas, and 55% in rural areas. Random digit dialing and random selection within households were utilized to ensure a representative sample of the population. Eighty percent of the sample was found to be favorable toward sex education in the public schools, while 20% was unfavorable. A majority of respondents in all religious groups including "fundamentalists" were favorable. Seventeen variables were found to be significant in the univariate analysis of the data; eight were not significant. In a multivariate analysis, three variables, age, Protestant denominational type and female employment, were shown to have predictive ability in determining favorability and unfavorability. Implications for building community support for sex education also are discussed.

Exponentially-Biased Ground-State Sampling of Quantum Annealing Machines with Transverse-Field Driving Hamiltonians

NASA Technical Reports Server (NTRS)

Mandra, Salvatore

2017-01-01

We study the performance of the D-Wave 2X quantum annealing machine on systems with well-controlled ground-state degeneracy. While obtaining the ground state of a spin-glass benchmark instance represents a difficult task, the gold standard for any optimization algorithm or machine is to sample all solutions that minimize the Hamiltonian with more or less equal probability. Our results show that while naive transverse-field quantum annealing on the D-Wave 2X device can find the ground-state energy of the problems, it is not well suited in identifying all degenerate ground-state configurations associated to a particular instance. Even worse, some states are exponentially suppressed, in agreement with previous studies on toy model problems [New J. Phys. 11, 073021 (2009)]. These results suggest that more complex driving Hamiltonians are needed in future quantum annealing machines to ensure a fair sampling of the ground-state manifold.

Verifying mixing in dilution tunnels How to ensure cookstove emissions samples are unbiased

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilson, Daniel L.; Rapp, Vi H.; Caubel, Julien J.

A well-mixed diluted sample is essential for unbiased measurement of cookstove emissions. Most cookstove testing labs employ a dilution tunnel, also referred to as a “duct,” to mix clean dilution air with cookstove emissions before sampling. It is important that the emissions be well-mixed and unbiased at the sampling port so that instruments can take representative samples of the emission plume. Some groups have employed mixing baffles to ensure the gaseous and aerosol emissions from cookstoves are well-mixed before reaching the sampling location [2, 4]. The goal of these baffles is to to dilute and mix the emissions stream withmore » the room air entering the fume hood by creating a local zone of high turbulence. However, potential drawbacks of mixing baffles include increased flow resistance (larger blowers needed for the same exhaust flow), nuisance cleaning of baffles as soot collects, and, importantly, the potential for loss of PM2.5 particles on the baffles themselves, thus biasing results. A cookstove emission monitoring system with baffles will collect particles faster than the duct’s walls alone. This is mostly driven by the available surface area for deposition by processes of Brownian diffusion (through the boundary layer) and turbophoresis (i.e. impaction). The greater the surface area available for diffusive and advection-driven deposition to occur, the greater the particle loss will be at the sampling port. As a layer of larger particle “fuzz” builds on the mixing baffles, even greater PM2.5 loss could occur. The micro structure of the deposited aerosol will lead to increased rates of particle loss by interception and a tendency for smaller particles to deposit due to impaction on small features of the micro structure. If the flow stream could be well-mixed without the need for baffles, these drawbacks could be avoided and the cookstove emissions sampling system would be more robust.« less

Local measurement of thermal conductivity and diffusivity.

PubMed

Hurley, David H; Schley, Robert S; Khafizov, Marat; Wendt, Brycen L

2015-12-01

Simultaneous measurement of local thermal diffusivity and conductivity is demonstrated on a range of ceramic samples. This was accomplished by measuring the temperature field spatial profile of samples excited by an amplitude modulated continuous wave laser beam. A thin gold film is applied to the samples to ensure strong optical absorption and to establish a second boundary condition that introduces an expression containing the substrate thermal conductivity. The diffusivity and conductivity are obtained by comparing the measured phase profile of the temperature field to a continuum based model. A sensitivity analysis is used to identify the optimal film thickness for extracting the both substrate conductivity and diffusivity. Proof of principle studies were conducted on a range of samples having thermal properties that are representatives of current and advanced accident tolerant nuclear fuels. It is shown that by including the Kapitza resistance as an additional fitting parameter, the measured conductivity and diffusivity of all the samples considered agreed closely with the literature values. A distinguishing feature of this technique is that it does not require a priori knowledge of the optical spot size which greatly increases measurement reliability and reproducibility.

Sample preparation composite and replicate strategy case studies for assay of solid oral drug products.

PubMed

Nickerson, Beverly; Harrington, Brent; Li, Fasheng; Guo, Michele Xuemei

2017-11-30

Drug product assay is one of several tests required for new drug products to ensure the quality of the product at release and throughout the life cycle of the product. Drug product assay testing is typically performed by preparing a composite sample of multiple dosage units to obtain an assay value representative of the batch. In some cases replicate composite samples may be prepared and the reportable assay value is the average value of all the replicates. In previously published work by Harrington et al. (2014) [5], a sample preparation composite and replicate strategy for assay was developed to provide a systematic approach which accounts for variability due to the analytical method and dosage form with a standard error of the potency assay criteria based on compendia and regulatory requirements. In this work, this sample preparation composite and replicate strategy for assay is applied to several case studies to demonstrate the utility of this approach and its application at various stages of pharmaceutical drug product development. Copyright © 2017 Elsevier B.V. All rights reserved.

Evaluating performance of stormwater sampling approaches using a dynamic watershed model.

PubMed

Ackerman, Drew; Stein, Eric D; Ritter, Kerry J

2011-09-01

Accurate quantification of stormwater pollutant levels is essential for estimating overall contaminant discharge to receiving waters. Numerous sampling approaches exist that attempt to balance accuracy against the costs associated with the sampling method. This study employs a novel and practical approach of evaluating the accuracy of different stormwater monitoring methodologies using stormflows and constituent concentrations produced by a fully validated continuous simulation watershed model. A major advantage of using a watershed model to simulate pollutant concentrations is that a large number of storms representing a broad range of conditions can be applied in testing the various sampling approaches. Seventy-eight distinct methodologies were evaluated by "virtual samplings" of 166 simulated storms of varying size, intensity and duration, representing 14 years of storms in Ballona Creek near Los Angeles, California. The 78 methods can be grouped into four general strategies: volume-paced compositing, time-paced compositing, pollutograph sampling, and microsampling. The performances of each sampling strategy was evaluated by comparing the (1) median relative error between the virtually sampled and the true modeled event mean concentration (EMC) of each storm (accuracy), (2) median absolute deviation about the median or "MAD" of the relative error or (precision), and (3) the percentage of storms where sampling methods were within 10% of the true EMC (combined measures of accuracy and precision). Finally, costs associated with site setup, sampling, and laboratory analysis were estimated for each method. Pollutograph sampling consistently outperformed the other three methods both in terms of accuracy and precision, but was the most costly method evaluated. Time-paced sampling consistently underestimated while volume-paced sampling over estimated the storm EMCs. Microsampling performance approached that of pollutograph sampling at a substantial cost savings. The most efficient method for routine stormwater monitoring in terms of a balance between performance and cost was volume-paced microsampling, with variable sample pacing to ensure that the entirety of the storm was captured. Pollutograph sampling is recommended if the data are to be used for detailed analysis of runoff dynamics.

Report: EPA Needs to Fulfill Its Designated Responsibilities to Ensure Effective BioWatch Program

EPA Pesticide Factsheets

Report #2005-P-00012, March 23, 2005. EPA did not provide adequate oversight of the sampling operations to ensure quality assurance guidance was adhered to, potentially affecting the quality of the samples taken.

49 CFR 601.3 - General responsibilities.

Code of Federal Regulations, 2013 CFR

2013-10-01

... management of FTA-sponsored projects; representing the Administration before civil courts and administrative... Office of Civil Rights. Directed by a Director for Civil Rights, this office ensures full implementation of civil rights and equal opportunity initiatives by all recipients of FTA assistance, and ensures...

49 CFR 601.3 - General responsibilities.

Code of Federal Regulations, 2012 CFR

2012-10-01

... management of FTA-sponsored projects; representing the Administration before civil courts and administrative... Office of Civil Rights. Directed by a Director for Civil Rights, this office ensures full implementation of civil rights and equal opportunity initiatives by all recipients of FTA assistance, and ensures...

49 CFR 601.3 - General responsibilities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... management of FTA-sponsored projects; representing the Administration before civil courts and administrative... Office of Civil Rights. Directed by a Director for Civil Rights, this office ensures full implementation of civil rights and equal opportunity initiatives by all recipients of FTA assistance, and ensures...

49 CFR 601.3 - General responsibilities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... management of FTA-sponsored projects; representing the Administration before civil courts and administrative... Office of Civil Rights. Directed by a Director for Civil Rights, this office ensures full implementation of civil rights and equal opportunity initiatives by all recipients of FTA assistance, and ensures...

A stochastic diffusion process for Lochner's generalized Dirichlet distribution

DOE PAGES

Bakosi, J.; Ristorcelli, J. R.

2013-10-01

The method of potential solutions of Fokker-Planck equations is used to develop a transport equation for the joint probability of N stochastic variables with Lochner’s generalized Dirichlet distribution as its asymptotic solution. Individual samples of a discrete ensemble, obtained from the system of stochastic differential equations, equivalent to the Fokker-Planck equation developed here, satisfy a unit-sum constraint at all times and ensure a bounded sample space, similarly to the process developed in for the Dirichlet distribution. Consequently, the generalized Dirichlet diffusion process may be used to represent realizations of a fluctuating ensemble of N variables subject to a conservation principle.more » Compared to the Dirichlet distribution and process, the additional parameters of the generalized Dirichlet distribution allow a more general class of physical processes to be modeled with a more general covariance matrix.« less

Development and Validation of a Musculoskeletal Model of the Fully Articulated Thoracolumbar Spine and Rib Cage

PubMed Central

Bruno, Alexander G.; Bouxsein, Mary L.; Anderson, Dennis E.

2015-01-01

We developed and validated a fully articulated model of the thoracolumbar spine in opensim that includes the individual vertebrae, ribs, and sternum. To ensure trunk muscles in the model accurately represent muscles in vivo, we used a novel approach to adjust muscle cross-sectional area (CSA) and position using computed tomography (CT) scans of the trunk sampled from a community-based cohort. Model predictions of vertebral compressive loading and trunk muscle tension were highly correlated to previous in vivo measures of intradiscal pressure (IDP), vertebral loading from telemeterized implants and trunk muscle myoelectric activity recorded by electromyography (EMG). PMID:25901907

The NOAA Scientific Computing System Data Assembly Center

NASA Astrophysics Data System (ADS)

Suchdeve, K. L.; Smith, S. R.; Van Waes, M.

2016-02-01

The Scientific Computing System (SCS) Data Assembly Center (DAC) was established in 2014 by the Office of Marine and Aviation Operations (OMAO) to evaluate the quality of full-resolution (sampling on the order of once per second) data collected by SCS onboard NOAA-operated research vessels. The SCS data are nominally transferred from the vessel to the National Centers for Environmental Information (NCEI) soon after the completion of each cruise and are complimented with detailed cruise metadata from OMAO. The authors will describe tools developed by the SCS DAC to monitor the timeliness of SCS data delivery to NCEI and the completeness of the SCS packages received by NCEI (ensuring the package contains data for all enabled sensors on a given cruise). Feedback to OMAO and NCEI regarding the timeliness and data completeness will be outlined along with challenges encountered by the DAC as it works to develop automated quality assessment of the SCS data packages.Data collected by SCS on NOAA vessels represent a significant investment by the American taxpayer. The mission of the SCS DAC is to ensure that archived SCS data at NCEI are a complete record of the observations made on NOAA research cruises. Archival of complete SCS datasets at NCEI ensures these data are preserved for future generations of scientists, policy makers, and the public.

The Student Affairs Committee. Effective Committees. Board Basics.

ERIC Educational Resources Information Center

Goodale, Thomas G.

1997-01-01

Responsibilities of the college or university governing board's student affairs committee include representing students' interests in all policy decisions, ensuring provision of adequate financial resources to support a comprehensive student affairs program, ensuring that board policies keep pace with students' diverse and changing needs, and…

Tissue Sampling Guides for Porcine Biomedical Models.

PubMed

Albl, Barbara; Haesner, Serena; Braun-Reichhart, Christina; Streckel, Elisabeth; Renner, Simone; Seeliger, Frank; Wolf, Eckhard; Wanke, Rüdiger; Blutke, Andreas

2016-04-01

This article provides guidelines for organ and tissue sampling adapted to porcine animal models in translational medical research. Detailed protocols for the determination of sampling locations and numbers as well as recommendations on the orientation, size, and trimming direction of samples from ∼50 different porcine organs and tissues are provided in the Supplementary Material. The proposed sampling protocols include the generation of samples suitable for subsequent qualitative and quantitative analyses, including cryohistology, paraffin, and plastic histology; immunohistochemistry;in situhybridization; electron microscopy; and quantitative stereology as well as molecular analyses of DNA, RNA, proteins, metabolites, and electrolytes. With regard to the planned extent of sampling efforts, time, and personnel expenses, and dependent upon the scheduled analyses, different protocols are provided. These protocols are adjusted for (I) routine screenings, as used in general toxicity studies or in analyses of gene expression patterns or histopathological organ alterations, (II) advanced analyses of single organs/tissues, and (III) large-scale sampling procedures to be applied in biobank projects. Providing a robust reference for studies of porcine models, the described protocols will ensure the efficiency of sampling, the systematic recovery of high-quality samples representing the entire organ or tissue as well as the intra-/interstudy comparability and reproducibility of results. © The Author(s) 2016.

Non-terminal blood sampling techniques in guinea pigs.

PubMed

Birck, Malene M; Tveden-Nyborg, Pernille; Lindblad, Maiken M; Lykkesfeldt, Jens

2014-10-11

Guinea pigs possess several biological similarities to humans and are validated experimental animal models(1-3). However, the use of guinea pigs currently represents a relatively narrow area of research and descriptive data on specific methodology is correspondingly scarce. The anatomical features of guinea pigs are slightly different from other rodent models, hence modulation of sampling techniques to accommodate for species-specific differences, e.g., compared to mice and rats, are necessary to obtain sufficient and high quality samples. As both long and short term in vivo studies often require repeated blood sampling the choice of technique should be well considered in order to reduce stress and discomfort in the animals but also to ensure survival as well as compliance with requirements of sample size and accessibility. Venous blood samples can be obtained at a number of sites in guinea pigs e.g., the saphenous and jugular veins, each technique containing both advantages and disadvantages(4,5). Here, we present four different blood sampling techniques for either conscious or anaesthetized guinea pigs. The procedures are all non-terminal procedures provided that sample volumes and number of samples do not exceed guidelines for blood collection in laboratory animals(6). All the described methods have been thoroughly tested and applied for repeated in vivo blood sampling in studies within our research facility.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haudebourg, Raphael; Fichet, Pascal; Goutelard, Florence

The detection (location and quantification) of nuclear facilities to be dismantled possible contamination with low-range particles emitters ({sup 3}H, other low-energy β emitters, a emitters) remains a tedious and expensive task. Indeed, usual remote counters show a too low sensitivity to these non-penetrating radiations, while conventional wipe tests are irrelevant for fixed radioactivity evaluation. The only method to accurately measure activity levels consists in sampling and running advanced laboratory analyses (spectroscopy, liquid scintillation counting, pyrolysis...). Such measurements generally induce sample preparation, waste production (destructive analyses, solvents), nuclear material transportation, long durations, and significant labor mobilization. Therefore, the search for themore » limitation of their number and cost easily conflicts with the necessity to perform a dense screening for sampling (to maximize the representativeness of the samples), in installations of thousands of square meters (floors, wells, ceilings), plus furniture, pipes, and other wastes. To overcome this contradiction, Digital Autoradiography (D. A.) was re-routed from bio molecular research to radiological mapping of nuclear installations under dismantling and to waste and sample analysis. After in-situ exposure to the possibly-contaminated areas to investigate, commercial reusable radiosensitive phosphor screens (of a few 100 cm{sup 2}) were scanned in the proper laboratory device and sharp quantitative images of the radioactivity could be obtained. The implementation of geostatistical tools in the data processing software enabled the exhaustive characterization of concrete floors at a rate of 2 weeks / 100 m{sup 2}, at lowest costs. Various samples such as drilled cores, or tank and wood pieces, were also successfully evaluated with this method, for decisive results. Thanks to the accurate location of potential contamination spots, this approach ensures relevant and representative sampling for further laboratory analyses and should be inserted in the range of common tools used in dismantling. (authors)« less

The optical and near-infrared colors of galaxies, 1: The photometric data

NASA Technical Reports Server (NTRS)

Bershady, Matthew A.; Hereld, Mark; Kron, Richard G.; Koo, David C.; Munn, Jeffrey A.; Majewski, Steven R.

1994-01-01

We present optical and near-infrared photometry and spectroscopic redshifts of a well defined sample of 171 field galaxies selected from three high galactic latitude fields. This data set forms the basis for subsequent studies to characterize the trends, dispersion, and evolution of rest-frame colors and image structure. A subset of 143 galaxies constitutes a magnitude-limited sample to B approx. 19.9-20.75 (depending on field), with a median redshift of 0.14, and a maximum redshift of 0.54. This subset is statistically representative in its sampling of the apparent color distribution of galaxies. Thirty six galaxies were selected to have the reddest red-optical colors in two redshift intervals between 0.2 less than z less than 0.3. Photometric passbands are similar to U, B, V, I, and K, and sample galaxy spectral energy distributions between 0.37 and 2.2 micrometers in the observed frame, or down to 0.26 micrometers in the rest frame for the most distant galaxies. B and K images of the entire sample are assembled to form the first optical and near-infrared atlas of a statistically-representative sample of field galaxies. We discuss techniques for faint field-galaxy photometry, including a working definition of a total magnitude, and a method for matching magnitudes in different passbands and different seeing conditions to ensure reliable, integrated colors. Photographic saturation, which substantially affects the brightest 12% of our sample in the optical bands, is corrected with a model employing measured plate-density distributions for each galaxy, calibrated via similar measurements for stars as a function of known saturation level. Both the relative and absolute calibration of our photometry are demonstrated.

40 CFR 80.400 - What defenses apply to persons deemed liable for a violation of a prohibited act?

Code of Federal Regulations, 2010 CFR

2010-07-01

... sampling and testing program, as described in paragraph (d) of this section. A carrier may rely on the... sampling and testing by the branded refiner or importer to ensure compliance with such contractual... person must present evidence of the following: (1) A periodic sampling and testing program to ensure the...

Water-quality assessment of the Delmarva Peninsula, Delaware, Maryland, and Virginia; results of investigations, 1987-91

USGS Publications Warehouse

Shedlock, Robert J.; Denver, J.M.; Hayes, M.A.; Hamilton, P.A.; Koterba, M.T.; Bachman, L.J.; Phillips, P.J.; Banks, W.S.

1999-01-01

A regional ground-water-quality assessment of the Delmarva Peninsula was conducted as a pilot study for the U.S. Geological Survey's National Water-Quality Assessment (NAWQA) Program. The study focused on the surficial aquifer and used both existing data and new data collected between 1988 and 1991. The new water samples were analyzed for major ions, nutrients, radon, volatile organic compounds, and a suite of herbicides and insecticides commonly used on corn, soybeans, and small grains. Samples also were collected from wells completed in deeper, confined aquifers and from selected streams, and analyzed for most of these constituents. The study employed a multi-scale network design. Regional networks were chosen to provide broad geographic coverage of the study area and to ensure that the major hydrogeologic settings of the surficial aquifer were adequately represented. Both the existing data and the data from samples collected during the study showed that agricultural activities had affected the quality of water in the surficial aquifer over most of the Peninsula.

Compressive Sensing of Roller Bearing Faults via Harmonic Detection from Under-Sampled Vibration Signals

PubMed Central

Tang, Gang; Hou, Wei; Wang, Huaqing; Luo, Ganggang; Ma, Jianwei

2015-01-01

The Shannon sampling principle requires substantial amounts of data to ensure the accuracy of on-line monitoring of roller bearing fault signals. Challenges are often encountered as a result of the cumbersome data monitoring, thus a novel method focused on compressed vibration signals for detecting roller bearing faults is developed in this study. Considering that harmonics often represent the fault characteristic frequencies in vibration signals, a compressive sensing frame of characteristic harmonics is proposed to detect bearing faults. A compressed vibration signal is first acquired from a sensing matrix with information preserved through a well-designed sampling strategy. A reconstruction process of the under-sampled vibration signal is then pursued as attempts are conducted to detect the characteristic harmonics from sparse measurements through a compressive matching pursuit strategy. In the proposed method bearing fault features depend on the existence of characteristic harmonics, as typically detected directly from compressed data far before reconstruction completion. The process of sampling and detection may then be performed simultaneously without complete recovery of the under-sampled signals. The effectiveness of the proposed method is validated by simulations and experiments. PMID:26473858

Subthreshold and threshold DSM-IV generalized anxiety disorder in Singapore: Results from a nationally representative sample.

PubMed

Lee, Siau Pheng; Sagayadevan, Vathsala; Vaingankar, Janhavi Ajit; Chong, Siow Ann; Subramaniam, Mythily

2015-05-01

Previous nationally representative studies have reported prevalence of DSM-IV generalized anxiety disorder (GAD). However, subthreshold and threshold GAD expressions remain poorly understood. The current study examined the prevalence, correlates and co-morbidity of a broader diagnosis of GAD in Singapore. The Singapore Mental Health Study (SMHS) was an epidemiological survey conducted in the population (N=6616) aged 18 years and older. The Composite International Diagnostic Interview version 3.0 (CIDI 3.0) was used to establish mental disorder diagnoses. The lifetime prevalence for subthreshold GAD (2.1%) and threshold GAD (1.5%) in the current sample was found to be lower than in Western populations. Younger age group, Indian ethnicity, previously married, chronic physical conditions, and being unemployed were associated with higher odds of having more severe expression of generalized anxiety. The relatively lower prevalence rate of subthreshold GAD expression suggests possible cultural interferences in the reporting and manifestation of anxiety symptomatology. Despite the low prevalence, significant impacts on functioning and comorbidity among subthreshold generalized anxiety cases indicate the importance of early treatment to ensure a better prognosis. Copyright © 2015 Elsevier Ltd. All rights reserved.

78 FR 9766 - Individuals With Certain Criminal Convictions as Representative Payees

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-11

... the best way to protect our beneficiaries from persons whose criminal history indicates they may pose...'s best interest and to report the expenditures to us to ensure that the representative payee is... beneficiary's best interests. When we investigate, we look at factors such as the potential representative...

Temporal Wind Pairs for Space Launch Vehicle Capability Assessment and Risk Mitigation

NASA Technical Reports Server (NTRS)

Decker, Ryan K.; Barbre, Robert E., Jr.

2015-01-01

Space launch vehicles incorporate upper-level wind assessments to determine wind effects on the vehicle and for a commit to launch decision. These assessments make use of wind profiles measured hours prior to launch and may not represent the actual wind the vehicle will fly through. Uncertainty in the winds over the time period between the assessment and launch introduces uncertainty in assessment of vehicle controllability and structural integrity that must be accounted for to ensure launch safety. Temporal wind pairs are used in engineering development of allowances to mitigate uncertainty. Five sets of temporal wind pairs at various times (0.75, 1.5, 2, 3 and 4-hrs) at the United States Air Force Eastern Range and Western Range, as well as the National Aeronautics and Space Administration's Wallops Flight Facility are developed for use in upper-level wind assessments on vehicle performance. Historical databases are compiled from balloon-based and vertically pointing Doppler radar wind profiler systems. Various automated and manual quality control procedures are used to remove unacceptable profiles. Statistical analyses on the resultant wind pairs from each site are performed to determine if the observed extreme wind changes in the sample pairs are representative of extreme temporal wind change. Wind change samples in the Eastern Range and Western Range databases characterize extreme wind change. However, the small sample sizes in the Wallops Flight Facility databases yield low confidence that the sample population characterizes extreme wind change that could occur.

Temporal Wind Pairs for Space Launch Vehicle Capability Assessment and Risk Mitigation

NASA Technical Reports Server (NTRS)

Decker, Ryan K.; Barbre, Robert E., Jr.

2014-01-01

Space launch vehicles incorporate upper-level wind assessments to determine wind effects on the vehicle and for a commit to launch decision. These assessments make use of wind profiles measured hours prior to launch and may not represent the actual wind the vehicle will fly through. Uncertainty in the winds over the time period between the assessment and launch introduces uncertainty in assessment of vehicle controllability and structural integrity that must be accounted for to ensure launch safety. Temporal wind pairs are used in engineering development of allowances to mitigate uncertainty. Five sets of temporal wind pairs at various times (0.75, 1.5, 2, 3 and 4-hrs) at the United States Air Force Eastern Range and Western Range, as well as the National Aeronautics and Space Administration's Wallops Flight Facility are developed for use in upper-level wind assessments on vehicle performance. Historical databases are compiled from balloon-based and vertically pointing Doppler radar wind profiler systems. Various automated and manual quality control procedures are used to remove unacceptable profiles. Statistical analyses on the resultant wind pairs from each site are performed to determine if the observed extreme wind changes in the sample pairs are representative of extreme temporal wind change. Wind change samples in the Eastern Range and Western Range databases characterize extreme wind change. However, the small sample sizes in the Wallops Flight Facility databases yield low confidence that the sample population characterizes extreme wind change that could occur.

[Indicators and factors of influence on the long-term follow-up of psychogenic diseases--a comparison of extreme groups].

PubMed

Franz, M; Schellberg, D; Schepank, H

1995-02-01

The present investigation aimed at the identification of possible indicators of course, predictors, and etiologically relevant factors of psychogenic diseases. According to their complaints a sample of probands suffering from psychogenic impairment of medium degree (n = 240) was chosen out of a representative sample of an urban adult population (n = 528). This procedure should ensure a relatively high intraindividual variance of course of the criterion, since a sufficient variability of course seems improbable with chronic and severe psychogenic impaired or stabile healthy probands. Within 10 years the sample was investigated three times by psychodynamically trained physicians and psychologists. By means of cluster analysis the sample was subdivided in different types of course of psychogenic impairment. Both extreme types of course-the probands who showed the most positive and the most negative spontaneous longterm course-were investigated univariately and by means of a multivariate discriminant analysis with regard to potentially course determining variables. It became obvious that personality variables and conditions of early childhood considerably influenced the spontaneous longterm course of psychogenic impairment.

Emergency contraception in a public health emergency: exploring pharmacy availability in Brazil.

PubMed

Tavares, Marilia P; Foster, Angel M

2016-08-01

Dedicated progestin-only emergency contraceptive pills (ECPs) have been available with a prescription in Brazil since 1999. However, utilization of emergency contraception has been limited. We conducted a mystery client study at retail pharmacies in three regions to assess current availability. Using a predetermined client profile, we approached a random sample of chain and independent pharmacies in urban areas in the southeastern, northeastern and central-west regions. We documented product availability, price and the client-pharmacy representative interaction at each site. We analyzed these data with descriptive statistics and for content and themes. We visited 122 pharmacies in early 2016. All but three pharmacies (97.5%) had ECPs in stock at the time of the interaction and offered our client the medication without a prescription. In general, pharmacy representatives did not ask questions or provide our client with information about emergency contraception. When prompted, over one third of the pharmacy representatives (37.7%) inaccurately reported that levonorgestrel ECPs could only be used immediately or within 12, 24 or 48h from the time of intercourse. Despite the current regulatory status, our findings suggest that progestin-only ECPs are widely available without a prescription. Additional efforts to ensure that women have up-to-date and medically accurate information about progestin-only ECPs appear warranted. Our findings suggest that more work needs to be done to align national regulatory policies with international standards and evidence-based practices. The Zika virus epidemic has shined a spotlight on the importance of providing timely access to emergency contraception in Latin America. This public health emergency offers a window of opportunity to advance national policies and practices to ensure that Brazilian women have access to a full range of reproductive health services. Copyright © 2016 Elsevier Inc. All rights reserved.

Field testing hot water temperature reduction as an energy-saving measure--does the Legionella presence change in a clinic's plumbing system?

PubMed

Völker, Sebastian; Kistemann, Thomas

2015-01-01

Legionella spp. represent a significant health risk for humans. To ensure hygienically safe drinking water, technical guidelines recommend a central potable water hot (PWH) supply temperature of at least 60°C at the calorifier. In a clinic building we monitored whether slightly lowered temperatures in the PWH system led to a systemic change in the growth of these pathogens. In four separate phases we tested different scenarios concerning PWH supply temperatures and disinfection with chlorine dioxide (ClO2). In each phase, we took 5 sets of samples at 17 representative sampling points in the building's drinking water plumbing system. In total we collected 476 samples from the PWH system. All samples were tested (culture-based) for Legionella spp. and serogroups. Additionally, quantitative parameters at each sampling point were collected, which could possibly be associated with the presence of Legionella spp. (Pseudomonas aeruginsoa, heterotrophic plate count at 20°C and 36°C, temperatures, time until constant temperatures were reached, and chlorine dioxide concentration). The presence of Legionella spp. showed no significant reactions after reducing the PWH supply temperature from 63°C to 60°C and 57°C, as long as disinfection with ClO2 was maintained. After omitting the disinfectant, the PWH system showed statistically significant growth rates at 57°C. PWH temperatures which are permanently lowered to less than recommended values should be carefully accompanied by frequent testing, a thorough evaluation of the building's drinking water plumbing system, and hygiene expertise.

A Stochastic Diffusion Process for the Dirichlet Distribution

DOE PAGES

Bakosi, J.; Ristorcelli, J. R.

2013-03-01

The method of potential solutions of Fokker-Planck equations is used to develop a transport equation for the joint probability ofNcoupled stochastic variables with the Dirichlet distribution as its asymptotic solution. To ensure a bounded sample space, a coupled nonlinear diffusion process is required: the Wiener processes in the equivalent system of stochastic differential equations are multiplicative with coefficients dependent on all the stochastic variables. Individual samples of a discrete ensemble, obtained from the stochastic process, satisfy a unit-sum constraint at all times. The process may be used to represent realizations of a fluctuating ensemble ofNvariables subject to a conservation principle.more » Similar to the multivariate Wright-Fisher process, whose invariant is also Dirichlet, the univariate case yields a process whose invariant is the beta distribution. As a test of the results, Monte Carlo simulations are used to evolve numerical ensembles toward the invariant Dirichlet distribution.« less

Framework for Derivation of Water Quality Criteria Using the Biotic Ligand Model: Copper as a Case Study.

PubMed

Gondek, John C; Gensemer, Robert W; Claytor, Carrie A; Canton, Steven P; Gorsuch, Joseph W

2018-06-01

Acceptance of the Biotic Ligand Model (BLM) to derive aquatic life criteria, for metals in general and copper in particular, is growing amongst regulatory agencies worldwide. Thus, it is important to ensure that water quality data are used appropriately and consistently in deriving such criteria. Here we present a suggested BLM implementation framework (hereafter referred to as "the Framework") to help guide the decision-making process when designing sampling and analysis programs for use of the BLM to derive water quality criteria applied on a site-specific basis. Such a framework will help inform stakeholders on the requirements needed to derive BLM-based criteria, and thus, ensure the appropriate types and amount of data are being collected and interpreted. The Framework was developed for calculating BLM-based criteria when data are available from multiple sampling locations on a stream. The Framework aspires to promote consistency when applying the BLM across datasets of disparate water quality, data quantity, and spatial and temporal representativeness, and is meant to be flexible to maximize applicability over a wide range of scenarios. Therefore, the Framework allows for a certain level of interpretation and adjustment to address the issues unique to each dataset. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Driving an Industry: Medium and Heavy Duty Fuel Cell Electric Truck Component Sizing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kast, James; Marcinkoski, Jason; Vijayagopal, Ram

Medium and heavy duty (MD and HD respectively) vehicles are responsible for 26 percent of the total U.S. transportation petroleum consumption [1]. Hydrogen fuel cells have demonstrated value as part of a portfolio of strategies for reducing petroleum use and emissions from MD and HD vehicles. [2] [3], but their performance and range capabilities, and associated component sizing remain less clear when compared to other powertrains. This paper examines the suitability of converting a representative sample of MD and HD diesel trucks into Fuel Cell Electric Trucks (FCETs), while ensuring the same truck performance, in terms of range, payload, acceleration,more » speed, gradeability and fuel economy.« less

Are Your S's in Effect? Ensuring Culturally Responsive Physical Education Environments

ERIC Educational Resources Information Center

Culp, Brian

2010-01-01

Schools are rapidly becoming a kaleidoscope of ethnicities and cultures represented by demographic changes in America's schools. As educators in this era of change, a unique opportunity exists to ensure quality physical education for all students. Culturally responsive practices in the classroom can assist in minimizing students' alienation as…

Local measurement of thermal conductivity and diffusivity

DOE PAGES

Hurley, David H.; Schley, Robert S.; Khafizov, Marat; ...

2015-12-01

Simultaneous measurement of local thermal diffusivity and conductivity is demonstrated on a range of ceramic samples. This was accomplished by measuring the temperature field spatial profile of samples excited by an amplitude modulated continuous wave laser beam. A thin gold film is applied to the samples to ensure strong optical absorption and to establish a second boundary condition that introduces an expression containing the substrate thermal conductivity. The diffusivity and conductivity are obtained by comparing the measured phase profile of the temperature field to a continuum based model. A sensitivity analysis is used to identify the optimal film thickness formore » extracting the both substrate conductivity and diffusivity. Proof of principle studies were conducted on a range of samples having thermal properties that are representative of current and advanced accident tolerant nuclear fuels. It is shown that by including the Kapitza resistance as an additional fitting parameter, the measured conductivity and diffusivity of all the samples considered agree closely with literature values. Lastly, a distinguishing feature of this technique is that it does not require a priori knowledge of the optical spot size which greatly increases measurement reliability and reproducibility.« less

Local measurement of thermal conductivity and diffusivity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hurley, David H.; Schley, Robert S.; Khafizov, Marat

2015-12-15

Simultaneous measurement of local thermal diffusivity and conductivity is demonstrated on a range of ceramic samples. This was accomplished by measuring the temperature field spatial profile of samples excited by an amplitude modulated continuous wave laser beam. A thin gold film is applied to the samples to ensure strong optical absorption and to establish a second boundary condition that introduces an expression containing the substrate thermal conductivity. The diffusivity and conductivity are obtained by comparing the measured phase profile of the temperature field to a continuum based model. A sensitivity analysis is used to identify the optimal film thickness formore » extracting the both substrate conductivity and diffusivity. Proof of principle studies were conducted on a range of samples having thermal properties that are representatives of current and advanced accident tolerant nuclear fuels. It is shown that by including the Kapitza resistance as an additional fitting parameter, the measured conductivity and diffusivity of all the samples considered agreed closely with the literature values. A distinguishing feature of this technique is that it does not require a priori knowledge of the optical spot size which greatly increases measurement reliability and reproducibility.« less

Physicochemical equivalence of generic antihypertensive medicines (EQUIMEDS): protocol for a quality of medicines assessment

PubMed Central

Redfern, Julie; Adedoyin, Rufus Adesoji; Ofori, Sandra; Anchala, Raghupathy; Ajay, Vamadevan S; De Andrade, Luciano; Zelaya, Jose; Kaur, Harparkash; Balabanova, Dina; Sani, Mahmoud U

2016-01-01

Background Prevention and optimal management of hypertension in the general population is paramount to the achievement of the World Heart Federation (WHF) goal of reducing premature cardiovascular disease (CVD) mortality by 25% by the year 2025 and widespread access to good quality antihypertensive medicines is a critical component for achieving the goal. Despite research and evidence relating to other medicines such as antimalarials and antibiotics, there is very little known about the quality of generic antihypertensive medicines in low-income and middle-income countries. The aim of this study was to determine the physicochemical equivalence (percentage of active pharmaceutical ingredient, API) of generic antihypertensive medicines available in the retail market of a developing country. Methods An observational design will be adopted, which includes literature search, landscape assessment, collection and analysis of medicine samples. To determine physicochemical equivalence, a multistage sampling process will be used, including (1) identification of the 2 most commonly prescribed classes of antihypertensive medicines prescribed in Nigeria; (2) identification of a random sample of 10 generics from within each of the 2 most commonly prescribed classes; (3) a geographical representative sampling process to identify a random sample of 24 retail outlets in Nigeria; (4) representative sample purchasing, processing to assess the quality of medicines, storage and transport; and (5) assessment of the physical and chemical equivalence of the collected samples compared to the API in the relevant class. In total, 20 samples from each of 24 pharmacies will be tested (total of 480 samples). Discussion Availability of and access to quality antihypertensive medicines globally is therefore a vital strategy needed to achieve the WHF 25×25 targets. However, there is currently a scarcity of knowledge about the quality of antihypertensive medicines available in developing countries. Such information is important for enforcing and for ensuring the quality of antihypertensive medicines. PMID:28588941

Rumen Bacterial Community Composition in Holstein and Jersey Cows Is Different under Same Dietary Condition and Is Not Affected by Sampling Method

PubMed Central

Paz, Henry A.; Anderson, Christopher L.; Muller, Makala J.; Kononoff, Paul J.; Fernando, Samodha C.

2016-01-01

The rumen microbial community in dairy cows plays a critical role in efficient milk production. However, there is a lack of data comparing the composition of the rumen bacterial community of the main dairy breeds. This study utilizes 16S rRNA gene sequencing to describe the rumen bacterial community composition in Holstein and Jersey cows fed the same diet by sampling the rumen microbiota via the rumen cannula (Holstein cows) or esophageal tubing (both Holstein and Jersey cows). After collection of the rumen sample via esophageal tubing, particles attached to the strainer were added to the sample to ensure representative sampling of both the liquid and solid fraction of the rumen contents. Alpha diversity metrics, Chao1 and observed OTUs estimates, displayed higher (P = 0.02) bacterial richness in Holstein compared to Jersey cows and no difference (P > 0.70) in bacterial community richness due to sampling method. The principal coordinate analysis displayed distinct clustering of bacterial communities by breed suggesting that Holstein and Jersey cows harbor different rumen bacterial communities. Family level classification of most abundant (>1%) differential OTUs displayed that OTUs from the bacterial families Lachnospiraceae and p-2534-18B5 to be predominant in Holstein cows compared to Jersey cows. Additionally, OTUs belonging to family Prevotellaceae were differentially abundant in the two breeds. Overall, the results from this study suggest that the bacterial community between Holstein and Jersey cows differ and that esophageal tubing with collection of feed particles associated with the strainer provides a representative rumen sample similar to a sample collected via the rumen cannula. Thus, in future studies esophageal tubing with addition of retained particles can be used to collect rumen samples reducing the cost of cannulation and increasing the number of animals used in microbiome investigations, thus increasing the statistical power of rumen microbial community evaluations. PMID:27536291

20 CFR 404.2022 - Who may not serve as a representative payee?

Code of Federal Regulations, 2013 CFR

2013-04-01

... willing to help manage his benefits or to act as representative payee. Sharon has demonstrated that her... payee only to ensure receipt of her rent. If there is money left after payment of the rent, she will...

20 CFR 404.2022 - Who may not serve as a representative payee?

Code of Federal Regulations, 2014 CFR

2014-04-01

... willing to help manage his benefits or to act as representative payee. Sharon has demonstrated that her... payee only to ensure receipt of her rent. If there is money left after payment of the rent, she will...

20 CFR 416.622 - Who may not serve as a representative payee?

Code of Federal Regulations, 2012 CFR

2012-04-01

... willing to help manage his benefits or to act as representative payee. Sharon has demonstrated that her... payee only to ensure receipt of her rent. If there is money left after payment of the rent, she will...

20 CFR 404.2022 - Who may not serve as a representative payee?

Code of Federal Regulations, 2012 CFR

2012-04-01

... willing to help manage his benefits or to act as representative payee. Sharon has demonstrated that her... payee only to ensure receipt of her rent. If there is money left after payment of the rent, she will...

20 CFR 416.622 - Who may not serve as a representative payee?

Code of Federal Regulations, 2014 CFR

2014-04-01

... willing to help manage his benefits or to act as representative payee. Sharon has demonstrated that her... payee only to ensure receipt of her rent. If there is money left after payment of the rent, she will...

20 CFR 416.622 - Who may not serve as a representative payee?

Code of Federal Regulations, 2013 CFR

2013-04-01

... willing to help manage his benefits or to act as representative payee. Sharon has demonstrated that her... payee only to ensure receipt of her rent. If there is money left after payment of the rent, she will...

20 CFR 404.2022 - Who may not serve as a representative payee?

Code of Federal Regulations, 2011 CFR

2011-04-01

... willing to help manage his benefits or to act as representative payee. Sharon has demonstrated that her... payee only to ensure receipt of her rent. If there is money left after payment of the rent, she will...

Who are your public? A survey comparing the views of a population-based sample with those of a community-based public forum in Scotland.

PubMed

Emslie, Margaret J; Andrew, Jane; Angus, Val; Entwistle, Vikki; Walker, Kim

2005-03-01

This paper describes a questionnaire survey, carried out in the NHS Grampian area of NE Scotland. It compares responses from 84 members of a community-based public forum (39 of whom were sent questionnaires) and a random sample of 10,000 adults registered with general practices in Grampian (2,449 of whom were sent questionnaires). differences in demographic profiles and opinions about different feedback mechanisms (patient representative, telephone helpline and NHS feedback website) and their likely effectiveness in three different scenarios. 46% of community forum members consented to take part compared to 24% of the population sample. Younger people and residents in more deprived areas were under-represented in both groups. Community forum members were older (only one under 40 years of age), more likely to be retired and not in employment. Internet access was similar in both groups. Opinions about different systems of feeding back views to the NHS varied but community forum members were more likely to be positive in their opinions about the value of different feedback mechanisms and less likely to think they were 'a waste of NHS money'. Responses to three scenarios revealed similar opinions, but on some issues, there were key differences in the responses from the two groups. Community forum members were more likely to consider writing a letter as a means of getting something done about a problem and were more likely to talk to their GP if experiencing a problem than respondents in the main group. In general their responses were more positive towards the NHS. There is a need to ensure a broad basis for membership of public forums and/or proactively seek the views of groups that are under-represented if public forums are to be used to represent the views of the wider population and inform decision making in the NHS.

An integrated national mortality surveillance system for death registration and mortality surveillance, China.

PubMed

Liu, Shiwei; Wu, Xiaoling; Lopez, Alan D; Wang, Lijun; Cai, Yue; Page, Andrew; Yin, Peng; Liu, Yunning; Li, Yichong; Liu, Jiangmei; You, Jinling; Zhou, Maigeng

2016-01-01

In China, sample-based mortality surveillance systems, such as the Chinese Center for Disease Control and Prevention's disease surveillance points system and the Ministry of Health's vital registration system, have been used for decades to provide nationally representative data on health status for health-care decision-making and performance evaluation. However, neither system provided representative mortality and cause-of-death data at the provincial level to inform regional health service needs and policy priorities. Moreover, the systems overlapped to a considerable extent, thereby entailing a duplication of effort. In 2013, the Chinese Government combined these two systems into an integrated national mortality surveillance system to provide a provincially representative picture of total and cause-specific mortality and to accelerate the development of a comprehensive vital registration and mortality surveillance system for the whole country. This new system increased the surveillance population from 6 to 24% of the Chinese population. The number of surveillance points, each of which covered a district or county, increased from 161 to 605. To ensure representativeness at the provincial level, the 605 surveillance points were selected to cover China's 31 provinces using an iterative method involving multistage stratification that took into account the sociodemographic characteristics of the population. This paper describes the development and operation of the new national mortality surveillance system, which is expected to yield representative provincial estimates of mortality in China for the first time.

The Evolution of Tyrosine-Recombinase Elements in Nematoda

PubMed Central

Szitenberg, Amir; Koutsovoulos, Georgios; Blaxter, Mark L.; Lunt, David H.

2014-01-01

Transposable elements can be categorised into DNA and RNA elements based on their mechanism of transposition. Tyrosine recombinase elements (YREs) are relatively rare and poorly understood, despite sharing characteristics with both DNA and RNA elements. Previously, the Nematoda have been reported to have a substantially different diversity of YREs compared to other animal phyla: the Dirs1-like YRE retrotransposon was encountered in most animal phyla but not in Nematoda, and a unique Pat1-like YRE retrotransposon has only been recorded from Nematoda. We explored the diversity of YREs in Nematoda by sampling broadly across the phylum and including 34 genomes representing the three classes within Nematoda. We developed a method to isolate and classify YREs based on both feature organization and phylogenetic relationships in an open and reproducible workflow. We also ensured that our phylogenetic approach to YRE classification identified truncated and degenerate elements, informatively increasing the number of elements sampled. We identified Dirs1-like elements (thought to be absent from Nematoda) in the nematode classes Enoplia and Dorylaimia indicating that nematode model species do not adequately represent the diversity of transposable elements in the phylum. Nematode Pat1-like elements were found to be a derived form of another Pat1-like element that is present more widely in animals. Several sequence features used widely for the classification of YREs were found to be homoplasious, highlighting the need for a phylogenetically-based classification scheme. Nematode model species do not represent the diversity of transposable elements in the phylum. PMID:25197791

European dry cooling tower operating experience

DOE Office of Scientific and Technical Information (OSTI.GOV)

DeSteese, J.G.; Simhan, K.

1976-03-01

Interviews were held with representatives of major plants and equipment manufacturers to obtain current information on operating experience with dry cooling towers in Europe. The report documents the objectives, background, and organizational details of the study, and presents an itemized account of contacts made to obtain information. Plant selection was based on a merit index involving thermal capacity and length of service. A questionnaire was used to organize operational data, when available, into nine major categories of experience. Information was also solicited concerning the use of codes and standards to ensure the achievement of cooling tower performance. Several plant operatorsmore » provided finned-tube samples for metallographic analysis. Additionally, information on both operating experience and developing technology was supplied by European technical societies and research establishments. Information obtained from these contacts provides an updated and representative sample of European experience with dry cooling towers, which supplements some of the detailed reviews already available in the literature. In addition, the study presents categorized operating experience with installations which have not been reviewed so extensively, but nevertheless, have significant operational histories when ranked by the merit index. The contacts and interviews reported in the survey occurred between late March and October 1975. The study was motivated by the expressed interest of U.S. utility industry representatives who expect European experience to provide a basis of confidence that dry cooling is a reliable technology, applicable when necessary, to U.S. operating requirements.« less

The evolution of tyrosine-recombinase elements in Nematoda.

PubMed

Szitenberg, Amir; Koutsovoulos, Georgios; Blaxter, Mark L; Lunt, David H

2014-01-01

Transposable elements can be categorised into DNA and RNA elements based on their mechanism of transposition. Tyrosine recombinase elements (YREs) are relatively rare and poorly understood, despite sharing characteristics with both DNA and RNA elements. Previously, the Nematoda have been reported to have a substantially different diversity of YREs compared to other animal phyla: the Dirs1-like YRE retrotransposon was encountered in most animal phyla but not in Nematoda, and a unique Pat1-like YRE retrotransposon has only been recorded from Nematoda. We explored the diversity of YREs in Nematoda by sampling broadly across the phylum and including 34 genomes representing the three classes within Nematoda. We developed a method to isolate and classify YREs based on both feature organization and phylogenetic relationships in an open and reproducible workflow. We also ensured that our phylogenetic approach to YRE classification identified truncated and degenerate elements, informatively increasing the number of elements sampled. We identified Dirs1-like elements (thought to be absent from Nematoda) in the nematode classes Enoplia and Dorylaimia indicating that nematode model species do not adequately represent the diversity of transposable elements in the phylum. Nematode Pat1-like elements were found to be a derived form of another Pat1-like element that is present more widely in animals. Several sequence features used widely for the classification of YREs were found to be homoplasious, highlighting the need for a phylogenetically-based classification scheme. Nematode model species do not represent the diversity of transposable elements in the phylum.

78 FR 41492 - Buy America Policy

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-10

... domestic manufacturing process for any steel or iron products (including protective coatings) that are... industry representatives in ensuring that Federal funds were used to support domestic manufacturing. While... in the manufacturing industry. Also, a bill has been introduced in the House of Representatives (HR...

76 FR 11492 - Notice for Request for Nominations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-02

... members who represent disciplines and stakeholder groups such as: Disciplines [cir] Medicine (allopathic... primary care) Professional counseling Geriatrics Students, residents or fellows representing the following... advisory committee. Every effort is made to ensure that the views of women, ethnic and racial groups, and...

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR FREEZER MAINTENANCE AND TEMPERATURE VERIFICATION FOR SAMPLE INTEGRITY (BCO-L-25.0)

EPA Science Inventory

The purpose of this SOP is to ensure suitable temperature maintenance of freezers used for storage of samples. This procedure was followed to ensure consistent data retrieval during the Arizona NHEXAS project and the "Border" study. Keywords: freezers; operation.

The National H...

A post-mortem survey on end-of-life decisions using a representative sample of death certificates in Flanders, Belgium: research protocol

PubMed Central

Chambaere, Kenneth; Bilsen, Johan; Cohen, Joachim; Pousset, Geert; Onwuteaka-Philipsen, Bregje; Mortier, Freddy; Deliens, Luc

2008-01-01

Background Reliable studies of the incidence and characteristics of medical end-of-life decisions with a certain or possible life shortening effect (ELDs) are indispensable for an evidence-based medical and societal debate on this issue. This article presents the protocol drafted for the 2007 ELD Study in Flanders, Belgium, and outlines how the main aims and challenges of the study (i.e. making reliable incidence estimates of end-of-life decisions, even rare ones, and describing their characteristics; allowing comparability with past ELD studies; guaranteeing strict anonymity given the sensitive nature of the research topic; and attaining a sufficient response rate) are addressed in a post-mortem survey using a representative sample of death certificates. Study design Reliable incidence estimates are achievable by using large at random samples of death certificates of deceased persons in Flanders (aged one year or older). This entails the cooperation of the appropriate administrative authorities. To further ensure the reliability of the estimates and descriptions, especially of less prevalent end-of-life decisions (e.g. euthanasia), a stratified sample is drawn. A questionnaire is sent out to the certifying physician of each death sampled. The questionnaire, tested thoroughly and avoiding emotionally charged terms is based largely on questions that have been validated in previous national and European ELD studies. Anonymity of both patient and physician is guaranteed through a rigorous procedure, involving a lawyer as intermediary between responding physicians and researchers. To increase response we follow the Total Design Method (TDM) with a maximum of three follow-up mailings. Also, a non-response survey is conducted to gain insight into the reasons for lack of response. Discussion The protocol of the 2007 ELD Study in Flanders, Belgium, is appropriate for achieving the objectives of the study; as past studies in Belgium, the Netherlands, and other European countries have shown, strictly anonymous and thorough surveys among physicians using a large, stratified, and representative death certificate sample are most suitable in nationwide studies of incidence and characteristics of end-of-life decisions. There are however also some limitations to the study design. PMID:18752659

The World Coal Quality Inventory: South America

USGS Publications Warehouse

Karlsen, Alex W.; Tewalt, Susan J.; Bragg, Linda J.; Finkelman, Robert B.

2006-01-01

Executive Summary-Introduction: The concepts of a global environment and economy are strongly and irrevocably linked to global energy issues. Worldwide coal production and international coal trade are projected to increase during the next several decades in an international energy mix that is still strongly dependent on fossil fuels. Therefore, worldwide coal use will play an increasingly visible role in global environmental, economic, and energy forums. Policy makers require information on coal, including coal quality data, to make informed decisions regarding domestic coal resource allocation, import needs and export opportunities, foreign policy objectives, technology transfer policies, foreign investment prospects, environmental and health assessments, and byproduct use and disposal issues. The development of a worldwide, reliable, coal quality database would help ensure the most economically and environmentally efficient global use of coal. The U.S. Geological Survey (USGS), in cooperation with many agencies and scientists from the world's coal producing countries, originally undertook a project to obtain representative samples of coal from most of the world's producing coal provinces during a limited period of time (roughly 1998-2005), which is called the World Coal Quality Inventory (WoCQI). The multitude of producing coal mines, coal occurrences, or limited accessibility to sites in some countries can preclude collecting more than a single sample from a mine. In some areas, a single sample may represent an entire coal mining region or basin. Despite these limitations in sampling and uneven distribution of sample collection, the analytical results can still provide a general overview of world coal quality. The USGS intends to present the WoCQI data in reports and, when possible, in Geographic Information System (GIS) products that cover important coal bearing and producing regions.

Modeling Soil Organic Carbon Variation Along Climatic and Topographic Trajectories in the Central Andes

NASA Astrophysics Data System (ADS)

Gavilan, C.; Grunwald, S.; Quiroz, R.; Zhu, L.

2015-12-01

The Andes represent the largest and highest mountain range in the tropics. Geological and climatic differentiation favored landscape and soil diversity, resulting in ecosystems adapted to very different climatic patterns. Although several studies support the fact that the Andes are a vast sink of soil organic carbon (SOC) only few have quantified this variable in situ. Estimating the spatial distribution of SOC stocks in data-poor and/or poorly accessible areas, like the Andean region, is challenging due to the lack of recent soil data at high spatial resolution and the wide range of coexistent ecosystems. Thus, the sampling strategy is vital in order to ensure the whole range of environmental covariates (EC) controlling SOC dynamics is represented. This approach allows grasping the variability of the area, which leads to more efficient statistical estimates and improves the modeling process. The objectives of this study were to i) characterize and model the spatial distribution of SOC stocks in the Central Andean region using soil-landscape modeling techniques, and to ii) validate and evaluate the model for predicting SOC content in the area. For that purpose, three representative study areas were identified and a suite of variables including elevation, mean annual temperature, annual precipitation and Normalized Difference Vegetation Index (NDVI), among others, was selected as EC. A stratified random sampling (namely conditioned Latin Hypercube) was implemented and a total of 400 sampling locations were identified. At all sites, four composite topsoil samples (0-30 cm) were collected within a 2 m radius. SOC content was measured using dry combustion and SOC stocks were estimated using bulk density measurements. Regression Kriging was used to map the spatial variation of SOC stocks. The accuracy, fit and bias of SOC models was assessed using a rigorous validation assessment. This study produced the first comprehensive, geospatial SOC stock assessment in this undersampled region that serves as a baseline reference to assess potential impacts of climate and land use change.

[Techniques and strategy of pathological sampling in the diagnostic and therapeutic management of lung cancer].

PubMed

Remmelink, M; Sokolow, Y; Leduc, D

2015-04-01

Histopathology is key to the diagnosis and staging of lung cancer. This analysis requires tissue sampling from primary and/or metastatic lesions. The choice of sampling technique is intended to optimize diagnostic yield while avoiding unnecessarily invasive procedures. Recent developments in targeted therapy require increasingly precise histological and molecular characterization of the tumor. Therefore, pathologists must be economical with tissue samples to ensure that they have the opportunity to perform all the analyses required. More than ever, good communication between clinician, endoscopist or surgeon, and pathologist is essential. This is necessary to ensure that all participants in the process of lung cancer diagnosis collaborate to ensure that the appropriate number and type of biopsies are performed with the appropriate tissue sampling treatment. This will allow performance of all the necessary analyses leading to a more precise characterization of the tumor, and thus the optimal treatment for patients with lung cancer. Copyright © 2015 SPLF. Published by Elsevier Masson SAS. All rights reserved.

76 FR 71573 - Medicare Program; Request for Nominations for Members for the Medicare Evidence Development...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-18

... individual qualifications and not as representatives of professional associations or societies. We wish to... subspecialties, administrative medicine, public health, biological and physical sciences, epidemiology and... as representatives of professional associations or societies. We wish to ensure adequate...

Quality of Artemisinin-Containing Antimalarials in Tanzania's Private Sector—Results from a Nationally Representative Outlet Survey

PubMed Central

2015-01-01

Ensuring that artemisinin-containing antimalarials (ACAs) are of good quality is a key component of effective malaria treatment. There are concerns that a high proportion of ACAs are falsified or substandard, though estimates are rarely based on representative data. During a nationally representative survey in Tanzania, ACAs were purchased from private retail drug outlets, and the active pharmaceutical ingredient (API) was measured. All 1,737 ACAs contained the labeled artemisinin derivative, with 4.1% being outside the 85–115% artemisinin API range defined as acceptable quality. World Health Organization (WHO) prequalified drugs had 0.1 times the odds of being poor quality compared with non-prequalified ACAs for the artemisinin component. When partner components of combination therapies were also considered, 12.1% were outside the acceptable API range, and WHO prequalified ACAs had 0.04 times the odds of being poor quality. Although the prevalence of poor quality ACAs was lower than reported elsewhere, the minority of samples found to be substandard is a cause for concern. Improvements in quality could be achieved by increasing the predominance of WHO prequalified products in the market. Continued monitoring of quality standards is essential. PMID:25897065

Effect of variable rates of daily sampling of fly larvae on decomposition and carrion insect community assembly: implications for forensic entomology field study protocols.

PubMed

Michaud, Jean-Philippe; Moreau, Gaétan

2013-07-01

Experimental protocols in forensic entomology successional field studies generally involve daily sampling of insects to document temporal changes in species composition on animal carcasses. One challenge with that method has been to adjust the sampling intensity to obtain the best representation of the community present without affecting the said community. To this date, little is known about how such investigator perturbations affect decomposition-related processes. Here, we investigated how different levels of daily sampling of fly eggs and fly larvae affected, over time, carcass decomposition rate and the carrion insect community. Results indicated that a daily sampling of <5% of the egg and larvae volumes present on a carcass, a sampling intensity believed to be consistent with current accepted practices in successional field studies, had little effect overall. Higher sampling intensities, however, slowed down carcass decomposition, affected the abundance of certain carrion insects, and caused an increase in the volume of eggs laid by dipterans. This study suggests that the carrion insect community not only has a limited resilience to recurrent perturbations but that a daily sampling intensity equal to or <5% of the egg and larvae volumes appears adequate to ensure that the system is representative of unsampled conditions. Hence we propose that this threshold be accepted as best practice in future forensic entomology successional field studies.

Sample Design, Sample Augmentation, and Estimation for Wave 2 of the NSHAP

PubMed Central

English, Ned; Pedlow, Steven; Kwok, Peter K.

2014-01-01

Objectives. The sample for the second wave (2010) of National Social Life, Health, and Aging Project (NSHAP) was designed to increase the scientific value of the Wave 1 (2005) data set by revisiting sample members 5 years after their initial interviews and augmenting this sample where possible. Method. There were 2 important innovations. First, the scope of the study was expanded by collecting data from coresident spouses or romantic partners. Second, to maximize the representativeness of the Wave 2 data, nonrespondents from Wave 1 were again approached for interview in the Wave 2 sample. Results. The overall unconditional response rate for the Wave 2 panel was 74%; the conditional response rate of Wave 1 respondents was 89%; the conditional response rate of partners was 84%; and the conversion rate for Wave 1 nonrespondents was 26%. Discussion. The inclusion of coresident partners enhanced the study by allowing the examination of how intimate, household relationships are related to health trajectories and by augmenting the size of the NSHAP sample size for this and future waves. The uncommon strategy of returning to Wave 1 nonrespondents reduced potential bias by ensuring that to the extent possible the whole of the original sample forms the basis for the field effort. NSHAP Wave 2 achieved its field objectives of consolidating the panel, recruiting their resident spouses or romantic partners, and converting a significant proportion of Wave 1 nonrespondents. PMID:25360016

Towards robust and repeatable sampling methods in eDNA based studies.

PubMed

Dickie, Ian A; Boyer, Stephane; Buckley, Hannah; Duncan, Richard P; Gardner, Paul; Hogg, Ian D; Holdaway, Robert J; Lear, Gavin; Makiola, Andreas; Morales, Sergio E; Powell, Jeff R; Weaver, Louise

2018-05-26

DNA based techniques are increasingly used for measuring the biodiversity (species presence, identity, abundance and community composition) of terrestrial and aquatic ecosystems. While there are numerous reviews of molecular methods and bioinformatic steps, there has been little consideration of the methods used to collect samples upon which these later steps are based. This represents a critical knowledge gap, as methodologically sound field sampling is the foundation for subsequent analyses. We reviewed field sampling methods used for metabarcoding studies of both terrestrial and freshwater ecosystem biodiversity over a nearly three-year period (n = 75). We found that 95% (n = 71) of these studies used subjective sampling methods, inappropriate field methods, and/or failed to provide critical methodological information. It would be possible for researchers to replicate only 5% of the metabarcoding studies in our sample, a poorer level of reproducibility than for ecological studies in general. Our findings suggest greater attention to field sampling methods and reporting is necessary in eDNA-based studies of biodiversity to ensure robust outcomes and future reproducibility. Methods must be fully and accurately reported, and protocols developed that minimise subjectivity. Standardisation of sampling protocols would be one way to help to improve reproducibility, and have additional benefits in allowing compilation and comparison of data from across studies. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature.

PubMed

Tsujino, Soichiro; Tomizaki, Takashi

2016-05-06

Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-Å resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography.

Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature

NASA Astrophysics Data System (ADS)

Tsujino, Soichiro; Tomizaki, Takashi

2016-05-01

Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-Å resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography.

Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature

PubMed Central

Tsujino, Soichiro; Tomizaki, Takashi

2016-01-01

Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-Å resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography. PMID:27150272

Uncovering Predictors of Disagreement: Ensuring the Quality of Expert Ratings

ERIC Educational Resources Information Center

Hoth, Jessica; Schwarz, Björn; Kaiser, Gabriele; Busse, Andreas; König, Johannes; Blömeke, Sigrid

2016-01-01

Rating scales are a popular item format used in many types of assessments. Yet, defining which rating is correct often represents a challenge. Using expert ratings as benchmarks is one approach to ensuring the quality of a rating instrument. In this paper, such expert ratings are analyzed in detail taking a video-based test instrument of teachers'…

The international Genome sample resource (IGSR): A worldwide collection of genome variation incorporating the 1000 Genomes Project data

PubMed Central

Clarke, Laura; Fairley, Susan; Zheng-Bradley, Xiangqun; Streeter, Ian; Perry, Emily; Lowy, Ernesto; Tassé, Anne-Marie; Flicek, Paul

2017-01-01

The International Genome Sample Resource (IGSR; http://www.internationalgenome.org) expands in data type and population diversity the resources from the 1000 Genomes Project. IGSR represents the largest open collection of human variation data and provides easy access to these resources. IGSR was established in 2015 to maintain and extend the 1000 Genomes Project data, which has been widely used as a reference set of human variation and by researchers developing analysis methods. IGSR has mapped all of the 1000 Genomes sequence to the newest human reference (GRCh38), and will release updated variant calls to ensure maximal usefulness of the existing data. IGSR is collecting new structural variation data on the 1000 Genomes samples from long read sequencing and other technologies, and will collect relevant functional data into a single comprehensive resource. IGSR is extending coverage with new populations sequenced by collaborating groups. Here, we present the new data and analysis that IGSR has made available. We have also introduced a new data portal that increases discoverability of our data—previously only browseable through our FTP site—by focusing on particular samples, populations or data sets of interest. PMID:27638885

77 FR 9210 - International Pacific Halibut Commission Appointments

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-16

... duties, Commissioners represent the interests of the United States and all of its stakeholders in the... Pacific Halibut Commission (IPHC). This action is necessary to ensure that the interests of the United States and all of its stakeholders in the Pacific halibut fishery are adequately represented. The...

Using Video Conferencing to Deliver a Brief Motivational Intervention for Alcohol and Sex Risk to Emergency Department Patients: A Proof-of-Concept Pilot Study

PubMed Central

Celio, Mark A.; Mastroleo, Nadine R.; DiGuiseppi, Graham; Barnett, Nancy P.; Colby, Suzanne M.; Kahler, Christopher W.; Operario, Don; Suffoletto, Brian; Monti, Peter M.

2016-01-01

Brief motivational intervention (MI) is an efficacious approach to reduce heavy drinking and associated sexual risk behavior among Emergency Department (ED) patients, but the intensity of demands placed on ED staff makes the implementation of in-person MIs logistically challenging. This proof-of-concept pilot study examined the acceptability and logistic feasibility of using video-conferencing technology to deliver an MI targeting heavy drinking and risky sexual behavior to patients in an ED setting. Rigorous screening procedures were employed to ensure that the pilot sample represents the target portion of ED patients who would benefit from this multi-target MI. Mixed qualitative and quantitative data from a sample of seven ED patients (57% Female; Mage = 35 years) who received MI by video conference consistently demonstrated high levels of satisfaction, engagement, and acceptability. The observed completion rate supports logistic feasibility, and patient feedback identified methods to improve the experience by using high-definition hardware, ensuring stronger network connectivity, and effectively communicating information regarding protection of privacy. Post-intervention patient ratings and independent ratings of the audio-recorded sessions (using the Motivational Interviewing Skills Coding system) were very high, suggesting that intervention fidelity and MI adherence was not compromised by delivery modality. Collectively, these data suggest video conferencing is a viable technology that can be employed to implement brief evidence-based MIs in ED settings. PMID:28649188

A qualitative study to explore influences on general practitioners' decisions to prescribe new drugs.

PubMed

Jacoby, Ann; Smith, Monica; Eccles, Martin

2003-02-01

Ensuring appropriate prescribing is an important challenge for the health service, and the need for research that takes account of the reasons behind individual general practitioners' (GPs) prescribing decisions has been highlighted. To explore differences among GPs in their decisions to prescribe new drugs. Qualitative approach, using in-depth semistructured interviews. Northern and Yorkshire Health Authority Region. Participants were identified from a random sample of 520 GPs in a quantitative study of patterns of uptake of eight recently introduced drugs. Purposeful sampling ensured inclusion of GPs prescribing any of the eight drugs and working in a range of practice settings. Fifty-six GPs were interviewed, using a topic guide. Interviews were recorded on audiotape. Transcribed text was methodically coded and data were analysed by constantly comparing emerging themes. Both low and high prescribers shared a view of themselves as conservative in their prescribing behaviour. Low prescribers appeared to conform more strongly to group norms and identified a consensus among practice partners in prescribing and cost-consciousness. Conformism to group norms was represented by a commitment to practice formularies. High prescribers more often expressed themselves to be indifferent to drug costs and a shared practice ethos. A shift in the attitudes of some GPs is required before cost-effectiveness is routinely incorporated in drug prescribing. The promotion of rational prescribing is likely to be more successful if efforts are focused on GPs' appreciation of cost issues and attitudes towards shared decision-making and responsibility.

Using Video Conferencing to Deliver a Brief Motivational Intervention for Alcohol and Sex Risk to Emergency Department Patients: A Proof-of-Concept Pilot Study.

PubMed

Celio, Mark A; Mastroleo, Nadine R; DiGuiseppi, Graham; Barnett, Nancy P; Colby, Suzanne M; Kahler, Christopher W; Operario, Don; Suffoletto, Brian; Monti, Peter M

2017-01-01

Brief motivational intervention (MI) is an efficacious approach to reduce heavy drinking and associated sexual risk behavior among Emergency Department (ED) patients, but the intensity of demands placed on ED staff makes the implementation of in-person MIs logistically challenging. This proof-of-concept pilot study examined the acceptability and logistic feasibility of using video-conferencing technology to deliver an MI targeting heavy drinking and risky sexual behavior to patients in an ED setting. Rigorous screening procedures were employed to ensure that the pilot sample represents the target portion of ED patients who would benefit from this multi-target MI. Mixed qualitative and quantitative data from a sample of seven ED patients (57% Female; M age = 35 years) who received MI by video conference consistently demonstrated high levels of satisfaction, engagement, and acceptability. The observed completion rate supports logistic feasibility, and patient feedback identified methods to improve the experience by using high-definition hardware, ensuring stronger network connectivity, and effectively communicating information regarding protection of privacy. Post-intervention patient ratings and independent ratings of the audio-recorded sessions (using the Motivational Interviewing Skills Coding system) were very high, suggesting that intervention fidelity and MI adherence was not compromised by delivery modality. Collectively, these data suggest video conferencing is a viable technology that can be employed to implement brief evidence-based MIs in ED settings.

Systematic evaluation of matrix effects in hydrophilic interaction chromatography versus reversed phase liquid chromatography coupled to mass spectrometry.

PubMed

Periat, Aurélie; Kohler, Isabelle; Thomas, Aurélien; Nicoli, Raul; Boccard, Julien; Veuthey, Jean-Luc; Schappler, Julie; Guillarme, Davy

2016-03-25

Reversed phase liquid chromatography (RPLC) coupled to mass spectrometry (MS) is the gold standard technique in bioanalysis. However, hydrophilic interaction chromatography (HILIC) could represent a viable alternative to RPLC for the analysis of polar and/or ionizable compounds, as it often provides higher MS sensitivity and alternative selectivity. Nevertheless, this technique can be also prone to matrix effects (ME). ME are one of the major issues in quantitative LC-MS bioanalysis. To ensure acceptable method performance (i.e., trueness and precision), a careful evaluation and minimization of ME is required. In the present study, the incidence of ME in HILIC-MS/MS and RPLC-MS/MS was compared for plasma and urine samples using two representative sets of 38 pharmaceutical compounds and 40 doping agents, respectively. The optimal generic chromatographic conditions in terms of selectivity with respect to interfering compounds were established in both chromatographic modes by testing three different stationary phases in each mode with different mobile phase pH. A second step involved the assessment of ME in RPLC and HILIC under the best generic conditions, using the post-extraction addition method. Biological samples were prepared using two different sample pre-treatments, i.e., a non-selective sample clean-up procedure (protein precipitation and simple dilution for plasma and urine samples, respectively) and a selective sample preparation, i.e., solid phase extraction for both matrices. The non-selective pretreatments led to significantly less ME in RPLC vs. HILIC conditions regardless of the matrix. On the contrary, HILIC appeared as a valuable alternative to RPLC for plasma and urine samples treated by a selective sample preparation. Indeed, in the case of selective sample preparation, the compounds influenced by ME were different in HILIC and RPLC, and lower and similar ME occurrence was generally observed in RPLC vs. HILIC for urine and plasma samples, respectively. The complementary of both chromatographic modes was also demonstrated, as ME was observed only scarcely for urine and plasma samples when selecting the most appropriate chromatographic mode. Copyright © 2015 Elsevier B.V. All rights reserved.

Validating Domain Ontologies: A Methodology Exemplified for Concept Maps

ERIC Educational Resources Information Center

Steiner, Christina M.; Albert, Dietrich

2017-01-01

Ontologies play an important role as knowledge domain representations in technology-enhanced learning and instruction. Represented in form of concept maps they are commonly used as teaching and learning material and have the potential to enhance positive educational outcomes. To ensure the effective use of an ontology representing a knowledge…

9 CFR 88.5 - Requirements at a slaughtering facility.

Code of Federal Regulations, 2010 CFR

2010-01-01

... COMMERCIAL TRANSPORTATION OF EQUINES FOR SLAUGHTER § 88.5 Requirements at a slaughtering facility. (a) Upon arrival at a slaughtering facility, the owner/shipper must: (1) Ensure that each equine has access to... representative; (3) Allow a USDA representative access to the equines for the purpose of examination; and (4...

11 CFR 110.12 - Candidate appearances on public educational institution premises.

Code of Federal Regulations, 2010 CFR

2010-01-01

... university, may make its facilities available to any candidate or political committee in the ordinary course...' representatives or representatives of political parties at which such individuals address or meet the institution... sessions, or similar communications in an academic setting, and makes reasonable efforts to ensure that the...

Using ancestry matching to combine family-based and unrelated samples for genome-wide association studies‡

PubMed Central

Crossett, Andrew; Kent, Brian P.; Klei, Lambertus; Ringquist, Steven; Trucco, Massimo; Roeder, Kathryn; Devlin, Bernie

2015-01-01

We propose a method to analyze family-based samples together with unrelated cases and controls. The method builds on the idea of matched case–control analysis using conditional logistic regression (CLR). For each trio within the family, a case (the proband) and matched pseudo-controls are constructed, based upon the transmitted and untransmitted alleles. Unrelated controls, matched by genetic ancestry, supplement the sample of pseudo-controls; likewise unrelated cases are also paired with genetically matched controls. Within each matched stratum, the case genotype is contrasted with control pseudo-control genotypes via CLR, using a method we call matched-CLR (mCLR). Eigenanalysis of numerous SNP genotypes provides a tool for mapping genetic ancestry. The result of such an analysis can be thought of as a multidimensional map, or eigenmap, in which the relative genetic similarities and differences amongst individuals is encoded in the map. Once constructed, new individuals can be projected onto the ancestry map based on their genotypes. Successful differentiation of individuals of distinct ancestry depends on having a diverse, yet representative sample from which to construct the ancestry map. Once samples are well-matched, mCLR yields comparable power to competing methods while ensuring excellent control over Type I error. PMID:20862653

Prevalence and antibiotic resistance of Enterococcus spp. isolated from retail cheese, ready-to-eat salads, ham, and raw meat.

PubMed

Pesavento, G; Calonico, C; Ducci, B; Magnanini, A; Lo Nostro, A

2014-08-01

Food specimens were analyzed in order to research Enterococcus spp.: 636 samples of raw meat (227 beef, 238 poultry, and 171 pork), 278 samples of cheese (110 fresh soft cheese and 168 mozzarella cheese), 214 samples of ready-to-eat salads, and 187 samples of ham. 312 strains of Enterococcus spp samples were isolated, then identified and submitted to susceptibility tests against 11 antimicrobial agents. The predominant species were Enterococcus faecalis in raw meat and Enterococcus faecium in retail products. Low percentages of microorganisms were resistant to vancomycin (3.53%), teicoplanin (2.24%), linezolid (0.32%), and amoxicillin in combination with clavulanic acid (0.32%). A high percentage of resistance was noted in E. faecalis at high level gentamicin (21.9%) and tetracycline (60.6%). In general, strains of E. faecalis were more resistant than E. faecium. Enterococci should be considered not only potential pathogens, but also a reservoir of genes encoding antibiotic resistance which can be transferred to other microorganisms. Continuous monitoring of their incidence and emerging resistance is important in order to identify foods which potentially represent a real risk to the population, and to ensure effective treatment of human enterococcal infections. Copyright © 2014 Elsevier Ltd. All rights reserved.

Comparing concentration methods: parasitrap® versus Kato-Katz for studying the prevalence of Helminths in Bengo province, Angola.

PubMed

Mirante, Clara; Clemente, Isabel; Zambu, Graciette; Alexandre, Catarina; Ganga, Teresa; Mayer, Carlos; Brito, Miguel

2016-09-01

Helminth intestinal parasitoses are responsible for high levels of child mortality and morbidity. Hence, the capacity to diagnose these parasitoses and consequently ensure due treatment represents a factor of great importance. The main objective of this study involves comparing two methods of concentration, parasitrap and Kato-Katz, for the diagnosis of intestinal parasitoses in faecal samples. Sample processing made recourse to two different concentration methods: the commercial parasitrap® method and the Kato-Katz method. We correspondingly collected a total of 610 stool samples from pre-school and school age children. The results demonstrate the incidence of helminth parasites in 32.8% or 32.3% of the sample collected depending on whether the concentration method applied was either the parasitrap method or the Kato-Katz method. We detected a relatively high percentage of samples testing positive for two or more species of helminth parasites. We would highlight that in searching for larvae the Kato-Katz method does not prove as appropriate as the parasitrap method. Both techniques prove easily applicable even in field working conditions and returning mutually agreeing results. This study concludes in favour of the need for deworming programs and greater public awareness among the rural populations of Angola.

Defining And Characterizing Sample Representativeness For DWPF Melter Feed Samples

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shine, E. P.; Poirier, M. R.

2013-10-29

Representative sampling is important throughout the Defense Waste Processing Facility (DWPF) process, and the demonstrated success of the DWPF process to achieve glass product quality over the past two decades is a direct result of the quality of information obtained from the process. The objective of this report was to present sampling methods that the Savannah River Site (SRS) used to qualify waste being dispositioned at the DWPF. The goal was to emphasize the methodology, not a list of outcomes from those studies. This methodology includes proven methods for taking representative samples, the use of controlled analytical methods, and datamore » interpretation and reporting that considers the uncertainty of all error sources. Numerous sampling studies were conducted during the development of the DWPF process and still continue to be performed in order to evaluate options for process improvement. Study designs were based on use of statistical tools applicable to the determination of uncertainties associated with the data needs. Successful designs are apt to be repeated, so this report chose only to include prototypic case studies that typify the characteristics of frequently used designs. Case studies have been presented for studying in-tank homogeneity, evaluating the suitability of sampler systems, determining factors that affect mixing and sampling, comparing the final waste glass product chemical composition and durability to that of the glass pour stream sample and other samples from process vessels, and assessing the uniformity of the chemical composition in the waste glass product. Many of these studies efficiently addressed more than one of these areas of concern associated with demonstrating sample representativeness and provide examples of statistical tools in use for DWPF. The time when many of these designs were implemented was in an age when the sampling ideas of Pierre Gy were not as widespread as they are today. Nonetheless, the engineers and statisticians used carefully thought out designs that systematically and economically provided plans for data collection from the DWPF process. Key shared features of the sampling designs used at DWPF and the Gy sampling methodology were the specification of a standard for sample representativeness, an investigation that produced data from the process to study the sampling function, and a decision framework used to assess whether the specification was met based on the data. Without going into detail with regard to the seven errors identified by Pierre Gy, as excellent summaries are readily available such as Pitard [1989] and Smith [2001], SRS engineers understood, for example, that samplers can be biased (Gy's extraction error), and developed plans to mitigate those biases. Experiments that compared installed samplers with more representative samples obtained directly from the tank may not have resulted in systematically partitioning sampling errors into the now well-known error categories of Gy, but did provide overall information on the suitability of sampling systems. Most of the designs in this report are related to the DWPF vessels, not the large SRS Tank Farm tanks. Samples from the DWPF Slurry Mix Evaporator (SME), which contains the feed to the DWPF melter, are characterized using standardized analytical methods with known uncertainty. The analytical error is combined with the established error from sampling and processing in DWPF to determine the melter feed composition. This composition is used with the known uncertainty of the models in the Product Composition Control System (PCCS) to ensure that the wasteform that is produced is comfortably within the acceptable processing and product performance region. Having the advantage of many years of processing that meets the waste glass product acceptance criteria, the DWPF process has provided a considerable amount of data about itself in addition to the data from many special studies. Demonstrating representative sampling directly from the large Tank Farm tanks is a difficult, if not unsolvable enterprise due to limited accessibility. However, the consistency and the adequacy of sampling and mixing at SRS could at least be studied under the controlled process conditions based on samples discussed by Ray and others [2012a] in Waste Form Qualification Report (WQR) Volume 2 and the transfers from Tanks 40H and 51H to the Sludge Receipt and Adjustment Tank (SRAT) within DWPF. It is important to realize that the need for sample representativeness becomes more stringent as the material gets closer to the melter, and the tanks within DWPF have been studied extensively to meet those needs.« less

Strategic thinking.

PubMed

Waters, Adele

As part of the Care campaign, nurse leaders and patients' representatives drew up ten 'priorities for action' that should be implemented by all healthcare providers. Second on the list is ensuring staffing levels are adequate. This article shows there is increasing understanding of the direct link between numbers of nursing staff and quality of care. Evidence-based tools are vital, given the imperative to ensure patient safety in a time of cost-cutting.

Prevalence and antimicrobial resistance of Listeria, Salmonella, and Yersinia species isolates in ducks and geese.

PubMed

Jamali, Hossein; Radmehr, Behrad; Ismail, Salmah

2014-04-01

The aims of this study were to determine the prevalence and antimicrobial resistance of Listeria, Salmonella, and Yersinia spp. isolated from duck and goose intestinal contents. A total of 471 samples, including 291 duck and 180 goose intestinal contents, were purchased from wet markets between November 2008 and July 2010. Listeria, Salmonella, and Yersinia spp. were isolated from 58 (12.3%), 107 (22.7%), and 80 (17%) of the samples, respectively. It was concluded that Listeria ivanovii, Salmonella Thompson, and Yersinia enterocolitica were the predominant serovars among Listeria, Salmonella, and Yersinia spp., respectively. Moreover, resistance to tetracycline was common in Listeria (48.3%) and Salmonella spp. (63.6%), whereas 51.3% of the Yersinia spp. isolates were resistant to cephalothin. Therefore, continued surveillance of the prevalence of the pathogens and also of emerging antibiotic resistance is needed to render possible the recognition of foods that may represent risks and also ensure the effective treatment of listeriosis, salmonellosis, and yersiniosis.

Spatial and temporal variation of macro-, meso- and microplastic abundance on a remote coral island of the Maldives, Indian Ocean.

PubMed

Imhof, Hannes K; Sigl, Robert; Brauer, Emilia; Feyl, Sabine; Giesemann, Philipp; Klink, Saskia; Leupolz, Kathrin; Löder, Martin G J; Löschel, Lena A; Missun, Jan; Muszynski, Sarah; Ramsperger, Anja F R M; Schrank, Isabella; Speck, Susan; Steibl, Sebastian; Trotter, Benjamin; Winter, Isabel; Laforsch, Christian

2017-03-15

Plastic debris is ubiquitous in the marine environment and the world's shores represent a major sink. However, knowledge about plastic abundance in remote areas is scarce. Therefore, plastic abundance was investigated on a small island of the Maldives. Plastic debris (>1mm) was sampled once in natural long-term accumulation zones at the north shore and at the high tide drift line of the south shore on seven consecutive days to quantify daily plastic accumulation. Reliable identification of plastic debris was ensured by FTIR spectroscopy. Despite the remoteness of the island a considerable amount of plastic debris was present. At both sites a high variability in plastic abundance on a spatial and temporal scale was observed, which may be best explained by environmental factors. In addition, our results show that snapshot sampling may deliver biased results and indicate that future monitoring programs should consider spatial and temporal variation of plastic deposition. Copyright © 2017 Elsevier Ltd. All rights reserved.

Results of calendar year 1994 monitor well inspection and maintenance program, Y-12 Plant, Oak Ridge, Tennessee

DOE Office of Scientific and Technical Information (OSTI.GOV)

McMaster, B.W.; Jones, S.B.; Sitzler, J.L.

1995-06-01

This document is a compendium of results of the calendar year 1994 Monitor Well Inspection and Maintenance Program at the Department of Energy Y-12 Plant in Oak Ridge, Tennessee. This report documents the work relating to well inspections and maintenance requests. Inspections are implemented in order to better assess the condition and maintenance needs of wells that are actively being monitored. Currently this approach calls for inspecting all wells on a routine (annual or triennial) basis which are: (1) in an active sampling program; (2) included in a hydrologic study; or (3) not in service, but not scheduled for pluggingmore » and abandonment. Routine inspections help to ensure that representative groundwater samples and hydrologic data are being collected, and contribute to the life expectancy of each well. This report formally presents well inspection and maintenance activities that were conducted at the Y-12 Plant during 1994. All inspections were conducted between April and December.« less

A novel visual pipework inspection system

NASA Astrophysics Data System (ADS)

Summan, Rahul; Jackson, William; Dobie, Gordon; MacLeod, Charles; Mineo, Carmelo; West, Graeme; Offin, Douglas; Bolton, Gary; Marshall, Stephen; Lille, Alexandre

2018-04-01

The interior visual inspection of pipelines in the nuclear industry is a safety critical activity conducted during outages to ensure the continued safe and reliable operation of plant. Typically, the video output by a manually deployed probe is viewed by an operator looking to identify and localize surface defects such as corrosion, erosion and pitting. However, it is very challenging to estimate the nature and extent of defects by viewing a large structure through a relatively small field of view. This work describes a new visual inspection system employing photogrammetry using a fisheye camera and a structured light system to map the internal geometry of pipelines by generating a photorealistic, geometrically accurate surface model. The error of the system output was evaluated through comparison to a ground truth laser scan (ATOS GOM Triple Scan) of a nuclear grade split pipe sample (stainless steel 304L, 80mm internal diameter) containing defects representative of the application - the error was found to be submillimeter across the sample.

Josephson coupling between superconducting islands on single- and bi-layer graphene

NASA Astrophysics Data System (ADS)

Mancarella, Francesco; Fransson, Jonas; Balatsky, Alexander

2016-05-01

We study the Josephson coupling of superconducting (SC) islands through the surface of single-layer graphene (SLG) and bilayer graphene (BLG) in the long-junction regime, as a function of the distance between the grains, temperature, chemical potential and external (transverse) gate-voltage. For SLG, we provide a comparison with existing literature. The proximity effect is analyzed through a Matsubara Green’s function approach. This represents the first step in a discussion of the conditions for the onset of a granular superconductivity within the film, made possible by Josephson currents flowing between superconductors. To ensure phase coherence over the 2D sample, a random spatial distribution can be assumed for the SC islands on the SLG sheet (or intercalating the BLG sheets). The tunable gate-voltage-induced band gap of BLG affects the asymptotic decay of the Josephson coupling-distance characteristic for each pair of SC islands in the sample, which results in a qualitatively strong field dependence of the relation between Berezinskii-Kosterlitz-Thouless transition critical temperature and gate voltage.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Marutzky, Sam; Farnham, Irene

The purpose of the Nevada National Security Site (NNSS) Integrated Sampling Plan (referred to herein as the Plan) is to provide a comprehensive, integrated approach for collecting and analyzing groundwater samples to meet the needs and objectives of the U.S. Department of Energy (DOE), National Nuclear Security Administration Nevada Field Office (NNSA/NFO) Underground Test Area (UGTA) Activity. Implementation of this Plan will provide high-quality data required by the UGTA Activity for ensuring public protection in an efficient and cost-effective manner. The Plan is designed to ensure compliance with the UGTA Quality Assurance Plan (QAP). The Plan’s scope comprises sample collectionmore » and analysis requirements relevant to assessing the extent of groundwater contamination from underground nuclear testing. This Plan identifies locations to be sampled by corrective action unit (CAU) and location type, sampling frequencies, sample collection methodologies, and the constituents to be analyzed. In addition, the Plan defines data collection criteria such as well-purging requirements, detection levels, and accuracy requirements; identifies reporting and data management requirements; and provides a process to ensure coordination between NNSS groundwater sampling programs for sampling of interest to UGTA. This Plan does not address compliance with requirements for wells that supply the NNSS public water system or wells involved in a permitted activity.« less

A workforce survey of Australian osteopathy: analysis of a nationally-representative sample of osteopaths from the Osteopathy Research and Innovation Network (ORION) project.

PubMed

Adams, Jon; Sibbritt, David; Steel, Amie; Peng, Wenbo

2018-05-10

Limited information is available regarding the profile and clinical practice characteristics of the osteopathy workforce in Australia. This paper reports such information by analysing data from a nationally-representative sample of Australian osteopaths. Data was obtained from a workforce survey of Australian osteopathy, investigating the characteristics of the practitioner, their practice, clinical management features and perceptions regarding research. The survey questionnaire was distributed to all registered osteopaths across Australia in 2016 as part of the Osteopathy Research and Innovation Network (ORION) project. A total of 992 Australian osteopaths participated in this study representing a response rate of 49.1%. The average age of the participants was 38.0 years with 58.1% being female and the majority holding a Bachelor or higher degree qualification related to the osteopathy professional. Approximately 80.0% of the osteopaths were practicing in an urban area, with most osteopaths working in multi-practitioner locations, having referral relationships with a range of health care practitioners, managing patients a number of musculoskeletal disorders, and providing multi-model treatment options. A total of 3.9 million patients were estimated to consult with osteopaths every year and an average of approximate 3.0 million hours were spent delivering osteopathy services per year. Further research is required to provide rich, in-depth examination regarding a range of osteopathy workforce issues which will help ensure safe, effective patient care to all receiving and providing treatments as part of the broader Australian health system.

Total Water Content Measurements with an Isokinetic Sampling Probe

NASA Technical Reports Server (NTRS)

Reehorst, Andrew L.; Miller, Dean R.; Bidwell, Colin S.

2010-01-01

The NASA Glenn Research Center has developed a Total Water Content (TWC) Isokinetic Sampling Probe. Since it is not sensitive to cloud water particle phase nor size, it is particularly attractive to support super-cooled large droplet and high ice water content aircraft icing studies. The instrument is comprised of the Sampling Probe, Sample Flow Control, and Water Vapor Measurement subsystems. Analysis and testing have been conducted on the subsystems to ensure their proper function and accuracy. End-to-end bench testing has also been conducted to ensure the reliability of the entire instrument system. A Stokes Number based collection efficiency correction was developed to correct for probe thickness effects. The authors further discuss the need to ensure that no condensation occurs within the instrument plumbing. Instrument measurements compared to facility calibrations from testing in the NASA Glenn Icing Research Tunnel are presented and discussed. There appears to be liquid water content and droplet size effects in the differences between the two measurement techniques.

The Genome Austria Tissue Bank (GATiB).

PubMed

Asslaber, M; Abuja, P M; Stark, K; Eder, J; Gottweis, H; Trauner, M; Samonigg, H; Mischinger, H J; Schippinger, W; Berghold, A; Denk, H; Zatloukal, K

2007-01-01

In the context of the Austrian Genome Program, a tissue bank is being established (Genome Austria Tissue Bank, GATiB) which is based on a collection of diseased and corresponding normal tissues representing a great variety of diseases at their natural frequency of occurrence from a non-selected Central European population of more than 700,000 patients. Major emphasis is put on annotation of archival tissue with comprehensive clinical data, including follow-up data. A specific IT infrastructure supports sample annotation, tracking of sample usage as well as sample and data storage. Innovative data protection tools were developed which prevent sample donor re-identification, particularly if detailed medical and genetic data are combined. For quality control of old archival tissues, new techniques were established to check RNA quality and antigen stability. Since 2003, GATiB has changed from a population-based tissue bank to a disease-focused biobank comprising major cancers such as colon, breast, liver, as well as metabolic liver diseases and organs affected by the metabolic syndrome. Prospectively collected tissues are associated with blood samples and detailed data on the sample donor's disease, lifestyle and environmental exposure, following standard operating procedures. Major emphasis is also placed on ethical, legal and social issues (ELSI) related to biobanks. A specific research project and an international advisory board ensure the proper embedding of GATiB in society and facilitate international networking. (c) 2007 S. Karger AG, Basel.

Performance Tested Method multiple laboratory validation study of ELISA-based assays for the detection of peanuts in food.

PubMed

Park, Douglas L; Coates, Scott; Brewer, Vickery A; Garber, Eric A E; Abouzied, Mohamed; Johnson, Kurt; Ritter, Bruce; McKenzie, Deborah

2005-01-01

Performance Tested Method multiple laboratory validations for the detection of peanut protein in 4 different food matrixes were conducted under the auspices of the AOAC Research Institute. In this blind study, 3 commercially available ELISA test kits were validated: Neogen Veratox for Peanut, R-Biopharm RIDASCREEN FAST Peanut, and Tepnel BioKits for Peanut Assay. The food matrixes used were breakfast cereal, cookies, ice cream, and milk chocolate spiked at 0 and 5 ppm peanut. Analyses of the samples were conducted by laboratories representing industry and international and U.S governmental agencies. All 3 commercial test kits successfully identified spiked and peanut-free samples. The validation study required 60 analyses on test samples at the target level 5 microg peanut/g food and 60 analyses at a peanut-free level, which was designed to ensure that the lower 95% confidence limit for the sensitivity and specificity would not be <90%. The probability that a test sample contains an allergen given a prevalence rate of 5% and a positive test result using a single test kit analysis with 95% sensitivity and 95% specificity, which was demonstrated for these test kits, would be 50%. When 2 test kits are run simultaneously on all samples, the probability becomes 95%. It is therefore recommended that all field samples be analyzed with at least 2 of the validated kits.

Auditing of chromatographic data.

PubMed

Mabie, J T

1998-01-01

During a data audit, it is important to ensure that there is clear documentation and an audit trail. The Quality Assurance Unit should review all areas, including the laboratory, during the conduct of the sample analyses. The analytical methodology that is developed should be documented prior to sample analyses. This is an important document for the auditor, as it is the instrumental piece used by the laboratory personnel to maintain integrity throughout the process. It is expected that this document will give insight into the sample analysis, run controls, run sequencing, instrument parameters, and acceptance criteria for the samples. The sample analysis and all supporting documentation should be audited in conjunction with this written analytical method and any supporting Standard Operating Procedures to ensure the quality and integrity of the data.

Teleconferencing: Cost optimization of satellite and ground systems for continuing progressional education and medical services

NASA Technical Reports Server (NTRS)

Dunn, D.; Lusignan, B.

1972-01-01

A set of analytical capabilities that are needed to assess the role satellite communications technology will play in public and other services was developed. It is user oriented in that it starts from descriptions of user demand and develops the ability to estimate the cost of satisfying that demand with the lowest cost communications system. To ensure that the analysis could cope with the complexities of the real users, two services were chosen as examples, continuing professional education and medical services. Telecommunications costs are effected greatly by demographic factors, involving distribution of users in urban areas and distances between towns in rural regions. For this reason the analytical tools were exercised on sample locations. San Jose, California and Denver, Colorado were used to represent an urban area and the Rocky Mountain states were used to represent a rural region. In assessing the range of satellite system costs, two example coverage areas were considered, one appropriate to cover the contiguous forty-eight states, a second appropriate to cover about one-third that area.

From global to local: exploring the relationship between parameters and behaviors in models of electrical excitability.

PubMed

Fletcher, Patrick; Bertram, Richard; Tabak, Joel

2016-06-01

Models of electrical activity in excitable cells involve nonlinear interactions between many ionic currents. Changing parameters in these models can produce a variety of activity patterns with sometimes unexpected effects. Further more, introducing new currents will have different effects depending on the initial parameter set. In this study we combined global sampling of parameter space and local analysis of representative parameter sets in a pituitary cell model to understand the effects of adding K (+) conductances, which mediate some effects of hormone action on these cells. Global sampling ensured that the effects of introducing K (+) conductances were captured across a wide variety of contexts of model parameters. For each type of K (+) conductance we determined the types of behavioral transition that it evoked. Some transitions were counterintuitive, and may have been missed without the use of global sampling. In general, the wide range of transitions that occurred when the same current was applied to the model cell at different locations in parameter space highlight the challenge of making accurate model predictions in light of cell-to-cell heterogeneity. Finally, we used bifurcation analysis and fast/slow analysis to investigate why specific transitions occur in representative individual models. This approach relies on the use of a graphics processing unit (GPU) to quickly map parameter space to model behavior and identify parameter sets for further analysis. Acceleration with modern low-cost GPUs is particularly well suited to exploring the moderate-sized (5-20) parameter spaces of excitable cell and signaling models.

Quality of Artemisinin-Containing Antimalarials in Tanzania's Private Sector--Results from a Nationally Representative Outlet Survey.

PubMed

Act Consortium Drug Quality Project Team And The Impact Study Team

2015-06-01

Ensuring that artemisinin-containing antimalarials (ACAs) are of good quality is a key component of effective malaria treatment. There are concerns that a high proportion of ACAs are falsified or substandard, though estimates are rarely based on representative data. During a nationally representative survey in Tanzania, ACAs were purchased from private retail drug outlets, and the active pharmaceutical ingredient (API) was measured. All 1,737 ACAs contained the labeled artemisinin derivative, with 4.1% being outside the 85-115% artemisinin API range defined as acceptable quality. World Health Organization (WHO) prequalified drugs had 0.1 times the odds of being poor quality compared with non-prequalified ACAs for the artemisinin component. When partner components of combination therapies were also considered, 12.1% were outside the acceptable API range, and WHO prequalified ACAs had 0.04 times the odds of being poor quality. Although the prevalence of poor quality ACAs was lower than reported elsewhere, the minority of samples found to be substandard is a cause for concern. Improvements in quality could be achieved by increasing the predominance of WHO prequalified products in the market. Continued monitoring of quality standards is essential. © The American Society of Tropical Medicine and Hygiene.

Department of Labor. Challenges in Ensuring Workforce Development and Worker Protection. Testimony before the Subcommittee on Human Resources, Committee on Government Reform and Oversight, House of Representatives.

ERIC Educational Resources Information Center

Joyner, Carlotta C.

In Fiscal Year 1997, the U.S. Department of Labor (DOL) has an estimated budget of $34.4 billion and is authorized 16,614 full-time-equivalent staff-years. DOL's many programs fall into two major categories: enhancing workers' skills through job training and ensuring worker protection. The DOL's work force development mission is being challenged…

Plans for Assessing Contractor Performance for the Camp Lemonnier Base Operations Support Contract Needed Improvement

DTIC Science & Technology

2015-08-27

ensure the functional assessment plans ( FAP ) for the three services contained all contractor work requiring assessment, measurable performance...review the FAPs after development and revisions to the FAPs , and did not require additional controls over the contractor assessment process in an...revised as follows: • Business Management System B-14.3 was updated to require the contract officer representative conduct a FAP review to ensure

Children in Care: A Report to the Representative for Children and Youth from the British Columbia Teachers' Federation

ERIC Educational Resources Information Center

British Columbia Teachers' Federation, 2009

2009-01-01

Teachers in the public schools of British Columbia have a special responsibility to help ensure that the aesthetic, emotional, intellectual, physical, and social development of children in care is met. Representatives of the British Columbia Teachers' Federation (BCTF) from the Aboriginal Education Advisory Committee, the Committee for Action on…

Rapid identification of regulated organic chemical compounds in toys using ambient ionization and a miniature mass spectrometry system.

PubMed

Guo, Xiangyu; Bai, Hua; Lv, Yueguang; Xi, Guangcheng; Li, Junfang; Ma, Xiaoxiao; Ren, Yue; Ouyang, Zheng; Ma, Qiang

2018-04-01

Rapid, on-site analysis was achieved through significantly simplified operation procedures for a wide variety of toy samples (crayon, temporary tattoo sticker, finger paint, modeling clay, and bubble solution) using a miniature mass spectrometry system with ambient ionization capability. The labor-intensive analytical protocols involving sample workup and chemical separation, traditionally required for MS-based analysis, were replaced by direct sampling analysis using ambient ionization methods. A Mini β ion trap miniature mass spectrometer was coupled with versatile ambient ionization methods, e.g. paper spray, extraction spray and slug-flow microextraction nanoESI for direct identification of prohibited colorants, carcinogenic primary aromatic amines, allergenic fragrances, preservatives and plasticizers from raw toy samples. The use of paper substrates coated with Co 3 O 4 nanoparticles allowed a great increase in sensitivity for paper spray. Limits of detection as low as 5μgkg -1 were obtained for target analytes. The methods being developed based on the integration of ambient ionization with miniature mass spectrometer represent alternatives to current in-lab MS analysis operation, and would enable fast, outside-the-lab screening of toy products to ensure children's safety and health. Copyright © 2017 Elsevier B.V. All rights reserved.

Methane yield in source-sorted organic fraction of municipal solid waste.

PubMed

Davidsson, Asa; Gruvberger, Christopher; Christensen, Thomas H; Hansen, Trine Lund; Jansen, Jes la Cour

2007-01-01

Treating the source-separated organic fraction of municipal solid waste (SS-OFMSW) by anaerobic digestion is considered by many municipalities in Europe as an environmentally friendly means of treating organic waste and simultaneously producing methane gas. Methane yield can be used as a parameter for evaluation of the many different systems that exist for sorting and pre-treating waste. Methane yield from the thermophilic pilot scale digestion of 17 types of domestically SS-OFMSW originating from seven full-scale sorting systems was found. The samples were collected during 1 year using worked-out procedures tested statistically to ensure representative samples. Each waste type was identified by its origin and by pre-sorting, collection and pre-treatment methods. In addition to the pilot scale digestion, all samples were examined by chemical analyses and methane potential measurements. A VS-degradation rate of around 80% and a methane yield of 300-400Nm(3) CH(4)/ton VS(in) were achieved with a retention time of 15 days, corresponding to approximately 70% of the methane potential. The different waste samples gave minor variation in chemical composition and thus also in methane yield and methane potential. This indicates that sorting and collection systems in the present study do not significantly affect the amount of methane produced per VS treated.

The international Genome sample resource (IGSR): A worldwide collection of genome variation incorporating the 1000 Genomes Project data.

PubMed

Clarke, Laura; Fairley, Susan; Zheng-Bradley, Xiangqun; Streeter, Ian; Perry, Emily; Lowy, Ernesto; Tassé, Anne-Marie; Flicek, Paul

2017-01-04

The International Genome Sample Resource (IGSR; http://www.internationalgenome.org) expands in data type and population diversity the resources from the 1000 Genomes Project. IGSR represents the largest open collection of human variation data and provides easy access to these resources. IGSR was established in 2015 to maintain and extend the 1000 Genomes Project data, which has been widely used as a reference set of human variation and by researchers developing analysis methods. IGSR has mapped all of the 1000 Genomes sequence to the newest human reference (GRCh38), and will release updated variant calls to ensure maximal usefulness of the existing data. IGSR is collecting new structural variation data on the 1000 Genomes samples from long read sequencing and other technologies, and will collect relevant functional data into a single comprehensive resource. IGSR is extending coverage with new populations sequenced by collaborating groups. Here, we present the new data and analysis that IGSR has made available. We have also introduced a new data portal that increases discoverability of our data-previously only browseable through our FTP site-by focusing on particular samples, populations or data sets of interest. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Mycotoxicological and palynological profiles of commercial brands of dried bee pollen.

PubMed

Deveza, Michele Valadares; Keller, Kelly Moura; Lorenzon, Maria Cristina Affonso; Nunes, Lucila Maria Teixeira; Sales, Érika Oliveira; Barth, Ortrud Monika

2015-01-01

Pollen is used in the human diet as a food supplement because of its high nutritional value; however, this product is prone to fungal contamination that could potentially generate toxins that are harmful to human health. This study aimed to verify the floral diversity of commercial brands of bee pollen and their mycotoxicological safety for human consumption. A total of 27 bee pollen samples were analyzed; these samples represented commercial brands, either showing an inspection seal or not, marketed in the State of Rio de Janeiro. The analyzed parameters included floral diversity through palynological analysis, water activity, fungal counts, identification and toxigenic profiles. The palynological analysis identified nine plant families, of which the Asteraceae was predominant. Analysis of hygienic quality based on fungal load showed that 92% of samples were reproved according to the commercial, sanitary, and food safety quality indicators. Aspergillus, Cladosporium and Penicillium were the most common genera. Toxigenic evaluation showed that 25% of the A. flavus strains produced aflatoxins. The high rate of contamination of products bearing an inspection seal emphasizes the need to monitor the entire procedure of bee pollen production, as well as to revise the current legislation to ensure safe commercialization of this product.

A Seroprevalence Study of Hepatitis B and C Virus Infections in a Hospitalized Population in Romania, an Opportunity for a Better National Prevention and Control Strategy.

PubMed

Popovici, Odette; Molnar, Geza B; Popovici, Florin; Janţă, Denisa; Pistol, Adriana; Azoicăi, Doina

2016-03-01

The most recent prevalence data for hepatitis B virus (HBV) infection in Romania came from an ESEN 2 study (2002), and from a Romanian population-based study performed in 2008. Most of the previous studies were regional and performed in specific groups (blood donors, pregnant women, institutionalized people, etc) and had limited representativeness at the national level, both for HBV and hepatitis C virus (HCV) infection. The scarcity of prevalence data for HBV and HCV infection coming from the routine surveillance was also considered. The aim of our study was to obtain overall and age group specific estimates of the prevalence of HBV and HCV infections markers in Romania, in order to recommend evidence-based public health interventions. The main outcome was the proportion of persons with HBV, HCV and HBV+HCV infection markers, overall and by age group and gender. Our seroprevalence study ensured national representativeness for the targeted hospitalized population. A prospective collection of serum samples in hospital laboratories was completed between September and November 2013, using a systematic sampling. The study respected the confidentiality of personal data. We calculated the sample size using EpiInfo7 and used Z test - Two-tailed probability for statistical significance. The overall prevalence data estimated in our study were HBc Ab 28%, HBs Ag 4.2%, HBs Ab regardless of titer 64.1%, HBs Ab in titer of at least 10 mUI/ml and negative HBc Ab 17.5%; HCV Ab 5.6%; HBc Ab and HCV Ab 2.8%, as markers of double infection. The overall prevalence data estimated in our study for HBs Ag (4.2%) and HCV Ab (5.6%) correspond to a medium endemicity based on the WHO criteria. The estimated prevalence of HBV and HCV infection markers in the study population should represent an opportunity for a better national prevention and control strategy.

Predictors of Quitting Attempts Among Tobacco Users in Bangladesh After a Communication Campaign to Launch Graphic Warning Labels on Packaging.

PubMed

Turk, Tahir; Newton, Fiona; Choudhury, Sohel; Islam, Md Shafiqul

2018-06-01

Tobacco use contributes to an estimated 14.6% of male and 5.7% of female deaths in Bangladesh. We examine the determinants of tobacco-related quit attempts among Bangladeshis with and without awareness of the synergized "People Behind the Packs" (PBTP) communication campaign used to support the introduction of pack-based graphic warning labels (GWLs) in 2016. Data from 1,796 adults were collected using multistage sampling and a cross-sectional face-to-face survey. Analyses used a normalized design weight to ensure representativeness to the national population of smokers within Bangladesh. For the overall sample, the multivariable logistic regression model revealed quit attempts were associated with having seen the pack-based GWLs, recalling ≥1 PBTP campaign message, higher levels of self-efficacy to quit, and recognizing more potential side-effects associated with using tobacco products. Conversely, the likelihood of quitting attempts were lower among dual tobacco users (relative to smokers) and those using tobacco at least daily (vs. less than daily). The hierarchical multivariable logistic regression model among those aware of ≥1 PBTP campaign message indicated quitting attempts were positively associated with recalling more of the campaign messages and discussing them with others. This national evaluation of pack-based GWLs and accompanying PBTP campaign within Bangladesh supports the efficacy of using synergized communication messages when introducing such labels. That quit attempts are more likely among those discussing PBTP campaign messages with others and recalling more PBTP campaign messages highlights the importance of ensuring message content is both memorable and engaging.

Youth self-report of child maltreatment in representative surveys: a systematic review

PubMed Central

Jessica, Laurin*; Caroline, Wallace*; Jasminka, Draca; Sarah, Aterman; Lil, Tonmyr

2018-01-01

Abstract Introduction: This systematic review identified population-representative youth surveys containing questions on self-reported child maltreatment. Data quality and ethical issues pertinent to maltreatment data collection were also examined. Methods: A search was conducted of relevant online databases for articles published from January 2000 through March 2016 reporting on population-representative data measuring child maltreatment. Inclusion criteria were established a priori; two reviewers independently assessed articles to ensure that the criteria were met and to verify the accuracy of extracted information. Results: A total of 73 articles reporting on 71 surveys met the inclusion criteria. A variety of strategies to ensure accurate information and to mitigate survey participants’ distress were reported. Conclusion: The extent to which efforts have been undertaken to measure the prevalence of child maltreatment reflects its perceived importance across the world. Data on child maltreatment can be effectively collected from youth, although our knowledge of best practices related to ethics and data quality is incomplete. PMID:29443484

A Surrogate-based Adaptive Sampling Approach for History Matching and Uncertainty Quantification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Weixuan; Zhang, Dongxiao; Lin, Guang

A critical procedure in reservoir simulations is history matching (or data assimilation in a broader sense), which calibrates model parameters such that the simulation results are consistent with field measurements, and hence improves the credibility of the predictions given by the simulations. Often there exist non-unique combinations of parameter values that all yield the simulation results matching the measurements. For such ill-posed history matching problems, Bayesian theorem provides a theoretical foundation to represent different solutions and to quantify the uncertainty with the posterior PDF. Lacking an analytical solution in most situations, the posterior PDF may be characterized with a samplemore » of realizations, each representing a possible scenario. A novel sampling algorithm is presented here for the Bayesian solutions to history matching problems. We aim to deal with two commonly encountered issues: 1) as a result of the nonlinear input-output relationship in a reservoir model, the posterior distribution could be in a complex form, such as multimodal, which violates the Gaussian assumption required by most of the commonly used data assimilation approaches; 2) a typical sampling method requires intensive model evaluations and hence may cause unaffordable computational cost. In the developed algorithm, we use a Gaussian mixture model as the proposal distribution in the sampling process, which is simple but also flexible to approximate non-Gaussian distributions and is particularly efficient when the posterior is multimodal. Also, a Gaussian process is utilized as a surrogate model to speed up the sampling process. Furthermore, an iterative scheme of adaptive surrogate refinement and re-sampling ensures sampling accuracy while keeping the computational cost at a minimum level. The developed approach is demonstrated with an illustrative example and shows its capability in handling the above-mentioned issues. Multimodal posterior of the history matching problem is captured and are used to give a reliable production prediction with uncertainty quantification. The new algorithm reveals a great improvement in terms of computational efficiency comparing previously studied approaches for the sample problem.« less

Qualification of serological infectious disease assays for the screening of samples from deceased tissue donors.

PubMed

Kitchen, A D; Newham, J A

2011-05-01

Whilst some of the assays used for serological screening of post-mortem blood samples from deceased tissue donors in some countries have been specifically validated by the manufacturer for this purpose, a significant number of those currently in use globally have not. Although specificity has previously been considered a problem in the screening of such samples, we believe that ensuring sensitivity is more important. The aim of this study was to validate a broader range of assays for the screening of post-mortem blood samples from deceased tissue donors. Six microplate immunoassays currently in use within National Health Service Blood and Transplant (NHSBT) for the screening of blood, tissue and stem cell donations were included. Representative samples from confirmed positive donors were titrated in screen negative post-mortem samples in parallel with normal pooled negative serum to determine if there was any inhibition with the post-mortem samples. There were no significant differences seen (P < 0.005) between the dilution curves obtained for the positive samples diluted in post-mortem samples and normal pooled sera. Although small numbers of samples were studied, it can be surmised that the post-mortem blood samples from deceased tissue donors, collected according to United Kingdom guidelines, are a suitable substrate for the assays evaluated. No diminution of reactivity was seen when dilution with sera from deceased donors was compared to dilution using pooled serum from live donors. In the absence of genuine low titre positive post-mortem samples, the use of samples spiked with various levels of target material provides a means of qualifying serological screening assays used by NHSBT for the screening of post-mortem blood samples from deceased tissue donors.

Optimizing primary care research participation: a comparison of three recruitment methods in data-sharing studies.

PubMed

Lord, Paul A; Willis, Thomas A; Carder, Paul; West, Robert M; Foy, Robbie

2016-04-01

Recruitment of representative samples in primary care research is essential to ensure high-quality, generalizable results. This is particularly important for research using routinely recorded patient data to examine the delivery of care. Yet little is known about how different recruitment strategies influence the characteristics of the practices included in research. We describe three approaches for recruiting practices to data-sharing studies, examining differences in recruitment levels and practice representativeness. We examined three studies that included varying populations of practices from West Yorkshire, UK. All used anonymized patient data to explore aspects of clinical practice. Recruitment strategies were 'opt-in', 'mixed opt-in and opt-out' and 'opt-out'. We compared aggregated practice data between recruited and not-recruited practices for practice list size, deprivation, chronic disease management, patient experience and rates of unplanned hospital admission. The opt-out strategy had the highest recruitment (80%), followed by mixed (70%) and opt-in (58%). Practices opting-in were larger (median 7153 versus 4722 patients, P = 0.03) than practices that declined to opt-in. Practices recruited by mixed approach were larger (median 7091 versus 5857 patients, P = 0.04) and had differences in the clinical quality measure (58.4% versus 53.9% of diabetic patients with HbA1c ≤ 59 mmol/mol, P < 0.01). We found no differences between practices recruited and not recruited using the opt-out strategy for any demographic or quality of care measures. Opt-out recruitment appears to be a relatively efficient approach to ensuring participation of typical general practices. Researchers should, with appropriate ethical safeguards, consider opt-out recruitment of practices for studies involving anonymized patient data sharing. © The Author 2016. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Slaughterhouses Fungal Burden Assessment: A Contribution for the Pursuit of a Better Assessment Strategy

PubMed Central

Viegas, Carla; Faria, Tiago; dos Santos, Mateus; Carolino, Elisabete; Sabino, Raquel; Quintal Gomes, Anita; Viegas, Susana

2016-01-01

In slaughterhouses, the biological risk is present not only from the direct or indirect contact with animal matter, but also from the exposure to bioaerosols. Fungal contamination was already reported from the floors and walls of slaughterhouses. This study intends to assess fungal contamination by cultural and molecular methods in poultry, swine/bovine and large animal slaughterhouses. Air samples were collected through an impaction method, while surface samples were collected by the swabbing method and subjected to further macro- and micro-scopic observations. In addition, we collected air samples using the impinger method in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely A. flavus, A. fumigatus and A. ochraceus complexes. Poultry and swine/bovine slaughterhouses presented each two sampling sites that surpass the guideline of 150 CFU/m3. Scopulariopsis candida was the most frequently isolated (59.5%) in poultry slaughterhouse air; Cladosporium sp. (45.7%) in the swine/bovine slaughterhouse; and Penicillium sp. (80.8%) in the large animal slaughterhouse. Molecular tools successfully amplified DNA from the A. fumigatus complex in six sampling sites where the presence of this fungal species was not identified by conventional methods. This study besides suggesting the indicators that are representative of harmful fungal contamination, also indicates a strategy as a protocol to ensure a proper characterization of fungal occupational exposure. PMID:27005642

Lesson 6: Signature Validation

EPA Pesticide Factsheets

Checklist items 13 through 17 are grouped under the Signature Validation Process, and represent CROMERR requirements that the system must satisfy as part of ensuring that electronic signatures it receives are valid.

MIP on the radar: the new drive to end Medicaid fraud.

PubMed

Fusto, John

2008-06-01

To ensure their billing integrity, providers should: Review every aspect of their billing integrity compliance program; Review the existing quality and methods of their statistical sampling; Examine an agency's or prosecutor's findings of loss to ensure accurate loss calculation.

USGS Laboratory Review Program Ensures Analytical Quality

USGS Publications Warehouse

Erdmann, David E.

1995-01-01

The USGS operates a review program for laboratories that analyze samples for USGS environmental investigations. This program has been effective in providing QA feedback to laboratories while ensuring that analytical data are consistent, of satisfactory quality, and meet the data objectives of the investigation.

Review of sampling, sample and data collection procedures in nursing research--An example of research on ethical climate as perceived by nurses.

PubMed

Suhonen, Riitta; Stolt, Minna; Katajisto, Jouko; Leino-Kilpi, Helena

2015-12-01

To report a review of quality regarding sampling, sample and data collection procedures of empirical nursing research of ethical climate studies where nurses were informants. Surveys are needed to obtain generalisable information about topics sensitive to nursing. Methodological quality of the studies is of key concern, especially the description of sampling and data collection procedures. Methodological literature review. Using the electronic MEDLINE database, empirical nursing research articles focusing on ethical climate were accessed in 2013 (earliest-22 November 2013). Using the search terms 'ethical' AND ('climate*' OR 'environment*') AND ('nurse*' OR 'nursing'), 376 citations were retrieved. Based on a four-phase retrieval process, 26 studies were included in the detailed analysis. Sampling method was reported in 58% of the studies, and it was random in a minority of the studies (26%). The identification of target sample and its size (92%) was reported, whereas justification for sample size was less often given. In over two-thirds (69%) of the studies with identifiable response rate, it was below 75%. A variety of data collection procedures were used with large amount of missing data about the details of who distributed, recruited and collected the questionnaires. Methods to increase response rates were seldom described. Discussion about nonresponse, representativeness of the sample and generalisability of the results was missing in many studies. This review highlights the methodological challenges and developments that need to be considered in ensuring the use of valid information in developing health care through research findings. © 2015 Nordic College of Caring Science.

A Translational Approach to Validate in Vivo Anti-tumor Effects of Chloroquine on Breast Cancer Risk

DTIC Science & Technology

2014-05-01

performed at one month post- activation to ensure appropriate accruals with the sampling method, Respondent Driven Sampling (RDS), was encouraging...Add another country/ year of service *Repeat these questions as many times as the RPCV has tours of service. Are you currently active duty...chains.  Interim  analyses  were  performed  at  one  and  two  months  post-­‐ activation  to  ensure  appropriate

32 CFR 536.105 - Responsibilities generally/international agreements claims.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Monitoring the reimbursement system to ensure that programs for the proper verification and certification of... liaison with sending State representatives and establishing procedures designed to carry out the...

The Polyethnic Competence of Class Teacher as a Resource for Ensuring the Psychological Security of Pupils in a Polycultural Educational Environment

ERIC Educational Resources Information Center

Kulikova, Tatyana I.; Shalaginova, Kseniya S.; Cherkasova, Svetlana A.

2017-01-01

In modern world the environment of any educational institution represents a spectrum of ethnoses, subcultures; and, thereafter, in a certain way, we can talk about it as the multicultural educational environment. Pupils who realize their national identity often demonstrate intolerance towards representatives of other nationalities which creates a…

Neutron Radiography Based Visualization and Profiling of Water Uptake in (Un)cracked and Autonomously Healed Cementitious Materials

PubMed Central

Van den Heede, Philip; Van Belleghem, Bjorn; Alderete, Natalia; Van Tittelboom, Kim; De Belie, Nele

2016-01-01

Given their low tensile strength, cement-based materials are very susceptible to cracking. These cracks serve as preferential pathways for corrosion inducing substances. For large concrete infrastructure works, currently available time-consuming manual repair techniques are not always an option. Often, one simply cannot reach the damaged areas and when making those areas accessible anyway (e.g., by redirecting traffic), the economic impacts involved would be enormous. Under those circumstances, it might be useful to have concrete with an embedded autonomous healing mechanism. In this paper, the effectiveness of incorporating encapsulated high and low viscosity polyurethane-based healing agents to ensure (multiple) crack healing has been investigated by means of capillary absorption tests on mortar while monitoring the time-dependent water ingress with neutron radiography. Overall visual interpretation and water front/sample cross-section area ratios as well as water profiles representing the area around the crack and their integrals do not show a preference for the high or low viscosity healing agent. Another observation is that in presence of two cracks, only one is properly healed, especially when using the latter healing agent. Exposure to water immediately after release of the healing agent stimulates the foaming reaction of the polyurethane and ensures a better crack closure. PMID:28773436

Sampling wild species to conserve genetic diversity

USDA-ARS?s Scientific Manuscript database

Sampling seed from natural populations of crop wild relatives requires choice of the locations to sample from and the amount of seed to sample. While this may seem like a simple choice, in fact careful planning of a collector’s sampling strategy is needed to ensure that a crop wild collection will ...

Comparing near-infrared conventional diffuse reflectance spectroscopy and hyperspectral imaging for determination of the bulk properties of solid samples by multivariate regression: determination of Mooney viscosity and plasticity indices of natural rubber.

PubMed

Juliano da Silva, Carlos; Pasquini, Celio

2015-01-21

Conventional reflectance spectroscopy (NIRS) and hyperspectral imaging (HI) in the near-infrared region (1000-2500 nm) are evaluated and compared, using, as the case study, the determination of relevant properties related to the quality of natural rubber. Mooney viscosity (MV) and plasticity indices (PI) (PI0 - original plasticity, PI30 - plasticity after accelerated aging, and PRI - the plasticity retention index after accelerated aging) of rubber were determined using multivariate regression models. Two hundred and eighty six samples of rubber were measured using conventional and hyperspectral near-infrared imaging reflectance instruments in the range of 1000-2500 nm. The sample set was split into regression (n = 191) and external validation (n = 95) sub-sets. Three instruments were employed for data acquisition: a line scanning hyperspectral camera and two conventional FT-NIR spectrometers. Sample heterogeneity was evaluated using hyperspectral images obtained with a resolution of 150 × 150 μm and principal component analysis. The probed sample area (5 cm(2); 24,000 pixels) to achieve representativeness was found to be equivalent to the average of 6 spectra for a 1 cm diameter probing circular window of one FT-NIR instrument. The other spectrophotometer can probe the whole sample in only one measurement. The results show that the rubber properties can be determined with very similar accuracy and precision by Partial Least Square (PLS) regression models regardless of whether HI-NIR or conventional FT-NIR produce the spectral datasets. The best Root Mean Square Errors of Prediction (RMSEPs) of external validation for MV, PI0, PI30, and PRI were 4.3, 1.8, 3.4, and 5.3%, respectively. Though the quantitative results provided by the three instruments can be considered equivalent, the hyperspectral imaging instrument presents a number of advantages, being about 6 times faster than conventional bulk spectrometers, producing robust spectral data by ensuring sample representativeness, and minimizing the effect of the presence of contaminants.

Associations between Child Disabilities and Caregiver Discipline and Violence in Low- and Middle-Income Countries

PubMed Central

Hendricks, Charlene; Lansford, Jennifer E.; Deater-Deckard, Kirby; Bornstein, Marc H.

2014-01-01

Using nationally representative samples of 45,964 2- to 9-year-old children and their primary caregivers in 17 developing countries, we sought to understand relations between children’s cognitive, language, sensory, and motor disabilities and caregivers’ use of discipline and violence. Primary caregivers reported on their child’s disabilities and whether they or anyone in their household had used nonviolent discipline, psychological aggression, and physical violence toward the target child and whether they believed that using corporal punishment is necessary. Logistic regression analyses supported the hypothesis that children with disabilities are treated more harshly than children without disabilities. The findings suggest that policies and interventions are needed to work toward the United Nations’ goals of ensuring that children with disabilities are protected from abuse and violence. PMID:23895329

Characterization of potassium bromide crystals grown in the aqueous solution of picric acid

NASA Astrophysics Data System (ADS)

Maheswari, J. Uma; Krishnan, C.; Kalyanaraman, S.; Selvarajan, P.

2016-12-01

Potassium bromide crystals were grown in the aqueous solution of picric acid by slow evaporation technique at room temperature. X-ray Diffraction (XRD) analysis ensures that the grown sample is in Fm3m space group and FCC structure. Energy Dispersive X-ray Spectroscopy (EDX) reveals the presence of elements in the title compound. UV-Vis-NIR spectrum reveals that the grown sample is a promising nonlinear optical (NLO) material. FTIR analysis confirms the functional groups present in the sample. The thermogravimetric (TG) and differential thermogravimetric (DTA) analyses ensure that the sample material is thermally stable up to 160 °C. The second harmonic efficiency of the sample is 1.3 times greater than that of standard KDP. The mechanical strength of the grown sample is estimated by Vickers microhardness tester. The electrical properties were investigated by impedance analysis and the results of various studies of the grown crystals are discussed.

A national reconnaissance of pharmaceuticals and other organic wastewater contaminants in the United States - I) Groundwater

USGS Publications Warehouse

Barnes, K.K.; Kolpin, D.W.; Furlong, E.T.; Zaugg, S.D.; Meyer, M.T.; Barber, L.B.

2008-01-01

As part of the continuing effort to collect baseline information on the environmental occurrence of pharmaceuticals, and other organic wastewater contaminants (OWCs) in the Nation's water resources, water samples were collected from a network of 47 groundwater sites across 18 states in 2000. All samples collected were analyzed for 65 OWCs representing a wide variety of uses and origins. Site selection focused on areas suspected to be susceptible to contamination from either animal or human wastewaters (i.e. down gradient of a landfill, unsewered residential development, or animal feedlot). Thus, sites sampled were not necessarily used as a source of drinking water but provide a variety of geohydrologic environments with potential sources of OWCs. OWCs were detected in 81% of the sites sampled, with 35 of the 65 OWCs being found at least once. The most frequently detected compounds include N,N-diethyltoluamide (35%, insect repellant), bisphenol A (30%, plasticizer), tri(2-chloroethyl) phosphate (30%, fire retardant), sulfamethoxazole (23%, veterinary and human antibiotic), and 4-octylphenol monoethoxylate (19%, detergent metabolite). Although sampling procedures were intended to ensure that all groundwater samples analyzed were indicative of aquifer conditions it is possible that detections of some OWCs could have resulted from leaching of well-construction materials and/or other site-specific conditions related to well construction and materials. Future research will be needed to identify those factors that are most important in determining the occurrence and concentrations of OWCs in groundwater.

Local synchronization of chaotic neural networks with sampled-data and saturating actuators.

PubMed

Wu, Zheng-Guang; Shi, Peng; Su, Hongye; Chu, Jian

2014-12-01

This paper investigates the problem of local synchronization of chaotic neural networks with sampled-data and actuator saturation. A new time-dependent Lyapunov functional is proposed for the synchronization error systems. The advantage of the constructed Lyapunov functional lies in the fact that it is positive definite at sampling times but not necessarily between sampling times, and makes full use of the available information about the actual sampling pattern. A local stability condition of the synchronization error systems is derived, based on which a sampled-data controller with respect to the actuator saturation is designed to ensure that the master neural networks and slave neural networks are locally asymptotically synchronous. Two optimization problems are provided to compute the desired sampled-data controller with the aim of enlarging the set of admissible initial conditions or the admissible sampling upper bound ensuring the local synchronization of the considered chaotic neural networks. A numerical example is used to demonstrate the effectiveness of the proposed design technique.

75 FR 39547 - Advisory Committee on Organ Transplantation; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-09

... bioethics, behavioral sciences, economics and statistics, as well as representatives of transplant..., ensuring that the system of organ transplantation is grounded in the best available medical science, and...

40 CFR 1042.835 - Certification of remanufactured engines.

Code of Federal Regulations, 2010 CFR

2010-07-01

... represent the deterioration expected in emissions over your engines' full useful life. (2) Collect emission... due diligence in ensuring that your system will not adversely affect safety or otherwise violate the...

[Food consumption and the nutritional status of schoolchildren of the Community of Madrid (CAENPE): general methodology and overall food consumption. Consumo de alimentos y estado nutricional de la población escolar].

PubMed

Vázquez, C; de Cos, A I; Martínez, P; Jaunsolo, M A; Román, E; Gómez, C; López, T; Hernáez, I; Seijas, V; Ramos, V

1995-01-01

The CAENPE study (Food Consumption and Nutritional State of the School Population) was a transversal observational study funded and promoted by the Directorate-General of Food Hygiene in the Ministry of Health, implemented in 1991-93, with the main aim of quantifying food consumption in the school population (6-14 years of age) in the Regional Community of Madrid, together with an anthropometric study and nutritional analysis of that population. This project sets our the General Methodology for the study, paying particular attention to the sampling design, to ensure that the sample is representative of the community, and the results of the overall consumption of food and its comparison with recommended diet and other population studies. Quantification shows a high and rising consumption of meat, meat products, sweets, snacks and prepared dishes, suitable consumption of eggs, legumes and fruit and a notable lack of greens, vegetables and potatoes. The basic results underline the need to introduce educational measures with practical effect on home and school menus.

Genetic testing and counseling of a recipient after bone marrow transplant from a sibling harboring a germline BRCA1 pathogenic mutation.

PubMed

Škerl, Petra; Krajc, Mateja; Blatnik, Ana; Novaković, Srdjan

2017-07-01

Allogenic bone marrow transplant recipients represent a unique challenge, when they are referred for genetic testing and counseling. When performing genetic testing, it is extremely important to ensure that the detected DNA mutations originate from the patients own DNA, and therefore the most appropriate and reliable biological sample for DNA isolation must be obtained. The aim of the present study was to present the germline testing and counseling approach utilized in a rare case of a chimeric woman who received an allogenic bone marrow transplant from a sibling with a germline BRCA1 pathogenic mutation. According to our results, hairs with follicles are a reliable and ready source of DNA in a patient whose blood is of allogenic bone marrow transplant donor origin. Compared with a fibroblast culture, which is more difficult to obtain, the hair follicles are much more accessible and hair sampling is less invasive for the patient. Genetic testing based on the other sources of DNA, such as buccal swabs, is questionable due to the known risk of donor DNA contamination.

Direct spectral analysis and determination of high content of carcinogenic bromine in bread using UV pulsed laser induced breakdown spectroscopy.

PubMed

Mehder, A O; Gondal, Mohammed A; Dastageer, Mohamed A; Habibullah, Yusuf B; Iqbal, Mohammed A; Oloore, Luqman E; Gondal, Bilal

2016-01-01

Laser induced breakdown spectroscopy (LIBS) was applied for the detection of carcinogenic elements like bromine in four representative brands of loaf bread samples and the measured bromine concentrations were 352, 157, 451, and 311 ppm, using Br I (827.2 nm) atomic transition line as the finger print atomic transition. Our LIBS system is equipped with a pulsed laser of wavelength 266 nm with energy 25 mJ pulse(-1), 8 ns pulse duration, 20 Hz repetition rate, and a gated ICCD camera. The LIBS system was calibrated with the standards of known concentrations in the sample (bread) matrix and such plot is linear in 20-500 ppm range. The capability of our system in terms of limit of detection and relative accuracy with respect to the standard inductively coupled plasma mass spectrometry (ICPMS) technique was evaluated and these values were 5.09 ppm and 0.01-0.05, respectively, which ensures the applicability of our system for Br trace level detection, and LIBS results are in excellent agreement with that of ICPMS results.

Analytical control test plan and microbiological methods for the water recovery test

NASA Technical Reports Server (NTRS)

Traweek, M. S. (Editor); Tatara, J. D. (Editor)

1994-01-01

Qualitative and quantitative laboratory results are important to the decision-making process. In some cases, they may represent the only basis for deciding between two or more given options or processes. Therefore, it is essential that handling of laboratory samples and analytical operations employed are performed at a deliberate level of conscientious effort. Reporting erroneous results can lead to faulty interpretations and result in misinformed decisions. This document provides analytical control specifications which will govern future test procedures related to all Water Recovery Test (WRT) Phase 3 activities to be conducted at the National Aeronautics and Space Administration/Marshall Space Flight Center (NASA/MSFC). This document addresses the process which will be used to verify analytical data generated throughout the test period, and to identify responsibilities of key personnel and participating laboratories, the chains of communication to be followed, and ensure that approved methodology and procedures are used during WRT activities. This document does not outline specifics, but provides a minimum guideline by which sampling protocols, analysis methodologies, test site operations, and laboratory operations should be developed.

Core Hunter 3: flexible core subset selection.

PubMed

De Beukelaer, Herman; Davenport, Guy F; Fack, Veerle

2018-05-31

Core collections provide genebank curators and plant breeders a way to reduce size of their collections and populations, while minimizing impact on genetic diversity and allele frequency. Many methods have been proposed to generate core collections, often using distance metrics to quantify the similarity of two accessions, based on genetic marker data or phenotypic traits. Core Hunter is a multi-purpose core subset selection tool that uses local search algorithms to generate subsets relying on one or more metrics, including several distance metrics and allelic richness. In version 3 of Core Hunter (CH3) we have incorporated two new, improved methods for summarizing distances to quantify diversity or representativeness of the core collection. A comparison of CH3 and Core Hunter 2 (CH2) showed that these new metrics can be effectively optimized with less complex algorithms, as compared to those used in CH2. CH3 is more effective at maximizing the improved diversity metric than CH2, still ensures a high average and minimum distance, and is faster for large datasets. Using CH3, a simple stochastic hill-climber is able to find highly diverse core collections, and the more advanced parallel tempering algorithm further increases the quality of the core and further reduces variability across independent samples. We also evaluate the ability of CH3 to simultaneously maximize diversity, and either representativeness or allelic richness, and compare the results with those of the GDOpt and SimEli methods. CH3 can sample equally representative cores as GDOpt, which was specifically designed for this purpose, and is able to construct cores that are simultaneously more diverse, and either are more representative or have higher allelic richness, than those obtained by SimEli. In version 3, Core Hunter has been updated to include two new core subset selection metrics that construct cores for representativeness or diversity, with improved performance. It combines and outperforms the strengths of other methods, as it (simultaneously) optimizes a variety of metrics. In addition, CH3 is an improvement over CH2, with the option to use genetic marker data or phenotypic traits, or both, and improved speed. Core Hunter 3 is freely available on http://www.corehunter.org .

Chinas Future SSBN Command and Control Structure

DTIC Science & Technology

2016-11-01

ndupress.ndu.edu SF No. 299 1 China’s ongoing modernization program is transforming the country’s nuclear arsenal from one consisting of a few...also be mediated by bureaucratic poli- tics, including the time-honored tradition of interservice rivalry. The emergent SSBN fleet may represent a prime...represent a reliable source of resources. China has dedicated substantial resources to undertaking a nuclear modernization program designed to ensure

Obliging Games

NASA Astrophysics Data System (ADS)

Chatterjee, Krishnendu; Horn, Florian; Löding, Christof

Graph games of infinite length provide a natural model for open reactive systems: one player (Eve) represents the controller and the other player (Adam) represents the environment. The evolution of the system depends on the decisions of both players. The specification for the system is usually given as an ω-regular language L over paths and Eve's goal is to ensure that the play belongs to L irrespective of Adam's behaviour.

Profile of and expenditure on morbidity and hospitalizations among elderly-Analysis of a nationally representative sample survey in India.

PubMed

Jeyashree, Kathiresan; Suliankatchi Abdulkader, Rizwan; Kathirvel, Soundappan; Chinnakali, Palanivel; Kumar Mv, Ajay

2018-01-01

Understanding morbidity pattern and associated expenditure is essential for implementation of appropriate healthcare and social security measures for the elderly. This study aims to assess the proportion of ailing persons (PAP) in the last 15days, the utilization of hospitalization services in the last 365days and the expenditure incurred for hospitalizations among the elderly in India. This study analysed data from a nationally representative survey by the National Sample Survey Office (NSSO) in 2014-15 on 36,480 rural and 29,452 urban households. Distribution of morbidity and in-patient health care utilisation were analysed by subgroups of sex, residence, wealth quintile and type of health care provider. All estimates were weighted to account for the complex sampling design. Among 27,245 elderly persons, 30.3% reported having suffered an ailment in the past 15days and 8% reported at least one hospitalisation episode in the last 365days. All quintiles, except the lowest, utilized private sector more than the public sector for hospitalisations. The distribution of PAP (Concentration Index (CI)=+0.11; +0.07,+0.15) and the utilization of hospitalisation services (CI=+0.18; +0.11,+0.25) were found to be significantly pro-rich. The median (IQR) expenditure on hospitalization was INR 7370 (2600, 18,060). The wealthiest quintile spent 3.1 times more than the poorest quintile on hospitalisation. Efforts to reduce inequity among elderly persons in health status and healthcare utilization should be integral to any strategy targeting achievement of third sustainable development goal- "ensuring healthy lives and promoting well-being for all at all ages". Copyright © 2017 Elsevier B.V. All rights reserved.

Evolution of efficient methods to sample lead sources, such as house dust and hand dust, in the homes of children

DOE Office of Scientific and Technical Information (OSTI.GOV)

Que Hee, S.S.; Peace, B.; Clark, C.S.

Efficient sampling methods to recover lead-containing house dust and hand dust have been evolved so that sufficient lead is collected for analysis and to ensure that correlational analyses linking these two parameters to blood lead are not dependent on the efficiency of sampling. Precise collection of loose house dust from a 1-unit area (484 cmS) with a Tygon or stainless steel sampling tube connected to a portable sampling pump (1.2 to 2.5 liters/min) required repetitive sampling (three times). The Tygon tube sampling technique for loose house dust <177 m in diameter was around 72% efficient with respect to dust weightmore » and lead collection. A representative house dust contained 81% of its total weight in this fraction. A single handwipe for applied loose hand dust was not acceptably efficient or precise, and at least three wipes were necessary to achieve recoveries of >80% of the lead applied. House dusts of different particle sizes <246 m adhered equally well to hands. Analysis of lead-containing material usually required at least three digestions/decantations using hot plate or microwave techniques to allow at least 90% of the lead to be recovered. It was recommended that other investigators validate their handwiping, house dust sampling, and digestion techniques to facilitate comparison of results across studies. The final methodology for the Cincinnati longitudinal study was three sampling passes for surface dust using a stainless steel sampling tube; three microwave digestion/decantations for analysis of dust and paint; and three wipes with handwipes with one digestion/decantation for the analysis of six handwipes together.« less

Twenty years trends and socio-demographic characteristics of HIV prevalence in women giving birth in Catalonia (Spain).

PubMed

Carnicer-Pont, Dolors; Montoliu, Alexandra; Marín, José Luis; Almeda, Jesús; González, Victoria; Muñoz, Rafael; Martínez, Carmen; Jané, Mireia; Casabona, Jordi

2015-01-01

Studies of the prevalence of HIV in sentinel populations are one of the key strategies to monitor the HIV epidemic. We describe HIV prevalence trends and identify differences across time in the sociodemographic characteristics of HIV-infected women giving birth in Catalonia. We used dried blood specimens, residual to newborn screening, which have been collected in Catalonia every 2 months since 1994. The total number of samples obtained until 2009 and in 2013 represented half of yearly newborns. From 2010 to 2012, the total number of samples obtained represented a quarter of yearly newborns. We studied the prevalence by year and place of current residence (Barcelona-city, cities>200,000 inhabitants and cities ≤ 200,000 inhabitants) and by the mother's birth country. A total of 624,912 infants were tested for HIV antibodies from January 1994 to December 2013. HIV prevalence trends among women giving birth in Catalonia decreased until 2007. Thereafter, there was a change to a steady trend until 2013. However, among foreign women giving birth and living in cities ≤ 200,000 inhabitants, the prevalence of HIV increased from 2007 to 2013. To ensure early identification and treatment of HIV-infected mothers, it is essential to maintain HIV surveillance programs and pre- and post-natal screening programs, both in Barcelona and in cities with 200,000 inhabitants or less, especially in immigrant women. Copyright © 2014 SESPAS. Published by Elsevier Espana. All rights reserved.

A pragmatic examination of active and passive recruitment methods to improve the reach of community lifestyle programs: The Talking Health Trial.

PubMed

Estabrooks, Paul; You, Wen; Hedrick, Valisa; Reinholt, Margaret; Dohm, Erin; Zoellner, Jamie

2017-01-19

A primary challenge for behavior change strategies is ensuring that interventions can be effective while also attracting a broad and representative sample of the target population. The purpose of this case-study was to report on (1) the reach of a randomized controlled trial targeting reduced sugary beverages, (2) potential participant characteristic differences based on active versus passive recruitment strategies, and (3) recruitment strategy cost. Demographic and recruitment information was obtained for 8 counties and for individuals screened for participation. Personnel activities and time were tracked. Costs were calculated and compared by active versus passive recruitment. Six-hundred and twenty, of 1,056 screened, individuals were eligible and 301enrolled (77% women; 90% white; mean income $21,981 ± 16,443). Eighty-two and 44% of those responding to passive and active methods, respectively, enrolled in the trial. However, active recruitment strategies yielded considerably more enrolled (active = 199; passive = 102) individuals. Passive recruitment strategies yielded a less representative sample in terms of gender (more women), education (higher), and income (higher; p's <0.05). The average cost of an actively recruited and enrolled participant was $278 compared to $117 for a passively recruited and enrolled participant. Though passive recruitment is more cost efficient it may reduce the reach of sugary drink reduction strategies in lower educated and economic residents in rural communities. Clinicaltrials.gov; ID: NCT02193009 , July 2014, retrospectively registered.

A Study to Develop a Timeline for Sequencing the Major Transitional Tasks in the Fort Sill Hospital Transition Plan

DTIC Science & Technology

1988-07-01

efforts. 7. Ensure that transition planning materials and manuals are prepared. 8. Ensure proper orientation of staff. 9. Ensure all involved parties...software package which will integrate with the project methodology: User’s manual that is "user friendly". Tutorial with sample data for learning the...program. Menus for direction and assistance. On-line help to avoid constant referral to the manual . Technical support available via telephone. Demo

Representativeness of direct observations selected using a work-sampling equation.

PubMed

Sharp, Rebecca A; Mudford, Oliver C; Elliffe, Douglas

2015-01-01

Deciding on appropriate sampling to obtain representative samples of behavior is important but not straightforward, because the relative duration of the target behavior may affect its observation in a given sampling interval. Work-sampling methods, which offer a way to adjust the frequency of sampling according to a priori or ongoing estimates of the behavior to achieve a preselected level of representativeness, may provide a solution. Full-week observations of 7 behaviors were conducted for 3 students with autism spectrum disorder and intellectual disabilities. Work-sampling methods were used to select momentary time samples from the full time-of-interest, which produced representative samples. However, work sampling required impractically high numbers of time samples to obtain representative samples. More practical momentary time samples produced less representative samples, particularly for low-duration behaviors. The utility and limits of work-sampling methods for applied behavior analysis are discussed. © Society for the Experimental Analysis of Behavior.

So many, yet few: Human resources for health in India.

PubMed

Rao, Krishna D; Bhatnagar, Aarushi; Berman, Peter

2012-08-13

In many developing countries, such as India, information on human resources in the health sector is incomplete and unreliable. This prevents effective workforce planning and management. This paper aims to address this deficit by producing a more complete picture of India's health workforce. Both the Census of India and nationally representative household surveys collect data on self-reported occupations. A representative sample drawn from the 2001 census was used to estimate key workforce indicators. Nationally representative household survey data and official estimates were used to compare and supplement census results. India faces a substantial overall deficit of health workers; the density of doctors, nurses and midwifes is a quarter of the 2.3/1000 population World Health Organization benchmark. Importantly, a substantial portion of the doctors (37%), particularly in rural areas (63%) appears to be unqualified. The workforce is composed of at least as many doctors as nurses making for an inefficient skill-mix. Women comprise only one-third of the workforce. Most workers are located in urban areas and in the private sector. States with poorer health and service use outcomes have a lower health worker density. Among the important human resources challenges that India faces is increasing the presence of qualified health workers in underserved areas and a more efficient skill mix. An important first step is to ensure the availability of reliable and comprehensive workforce information through live workforce registers.

Determinants of job satisfaction for novice nurse managers employed in hospitals.

PubMed

Djukic, Maja; Jun, Jin; Kovner, Christine; Brewer, Carol; Fletcher, Jason

Numbering close to 300,000 nurse managers represent the largest segment of the health care management workforce. Their effectiveness is, in part, influenced by their job satisfaction. We examined factors associated with job satisfaction of novice frontline nurse managers. We used a cross-sectional, correlational survey design. The sample consisted of responders to the fifth wave of a multiyear study of new nurses in 2013 (N = 1,392; response rate of 69%) who reported working as managers (n = 209). The parent study sample consisted of registered nurses who were licensed for the first time by exam 6-18 months prior in 1 of 51 selected metropolitan statistical areas and 9 rural areas across 34 U.S. states and the District of Columbia. We examined bivariate correlations between job satisfaction and 31 personal and structural variables. All variables significantly related to job satisfaction in bivariate analysis were included in a multivariate linear regression model. In addition, we tested the interaction effects of procedural justice and negative affectivity, autonomy, and organizational constraints on job satisfaction. The Cronbach's alphas for all multi-item scales ranged from .74 to .96. In the multivariate analysis, negative affectivity (β = -.169; p = .006) and procedural justice (β = .210; p = .016) were significantly correlated with job satisfaction. The combination of predictors in the model accounted for half of the variability in job satisfaction ratings (R = .51, adjusted R = .47; p <. 001). Health care executives who want to cultivate an effective novice frontline nurse manager workforce can best ensure their satisfaction by creating an organization with strong procedural justice. This could be achieved by involving managers in decision-making processes and ensuring transparency about how decisions that affect nursing are made.

Knowledge about pandemic influenza and compliance with containment measures among Australians.

PubMed

Eastwood, Keith; Durrheim, David; Francis, J Lynn; d'Espaignet, Edouard Tursan; Duncan, Sarah; Islam, Fakhrul; Speare, Rick

2009-08-01

To examine the level of stated compliance with public health pandemic influenza control measures and explore factors influencing cooperation for pandemic influenza control in Australia. A computer-assisted telephone interview survey was conducted by professional interviewers to collect information on the Australian public's knowledge of pandemic influenza and willingness to comply with public health control measures. The sample was randomly selected using an electronic database and printed telephone directories to ensure sample representativeness from all Australian states and territories. After we described pandemic influenza to the respondents to ensure they understood the significance of the issue, the questions on compliance were repeated and changes in responses were analysed with McNemar's test for paired data Only 23% of the 1166 respondents demonstrated a clear understanding of the term 'pandemic influenza'. Of those interviewed, 94.1% reported being willing to comply with home quarantine; 94.2%, to avoid public events; and 90.7%, to postpone social gatherings. After we explained the meaning of 'pandemic' to interviewees, stated compliance increased significantly (to 97.5%, 98.3% and 97.2% respectively). Those who reported being unfamiliar with the term 'pandemic influenza,' male respondents and employed people not able to work from home were less willing to comply. In Australia, should the threat arise, compliance with containment measures against pandemic influenza is likely to be high, yet it could be further enhanced through a public education programme conveying just a few key messages. A basic understanding of pandemic influenza is associated with stated willingness to comply with containment measures. Investing now in promoting measures to prepare for a pandemic or other health emergency will have considerable value.

Successful adaption of a forensic toxicological screening workflow employing nontargeted liquid chromatography-tandem mass spectrometry to water analysis.

PubMed

Steger, Julia; Arnhard, Kathrin; Haslacher, Sandra; Geiger, Klemens; Singer, Klaus; Schlapp, Michael; Pitterl, Florian; Oberacher, Herbert

2016-04-01

Forensic toxicology and environmental water analysis share the common interest and responsibility in ensuring comprehensive and reliable confirmation of drugs and pharmaceutical compounds in samples analyzed. Dealing with similar analytes, detection and identification techniques should be exchangeable between scientific disciplines. Herein, we demonstrate the successful adaption of a forensic toxicological screening workflow employing nontargeted LC/MS/MS under data-dependent acquisition control and subsequent database search to water analysis. The main modification involved processing of an increased sample volume with SPE (500 mL vs. 1-10 mL) to reach LODs in the low ng/L range. Tandem mass spectra acquired with a qTOF instrument were submitted to database search. The targeted data mining strategy was found to be sensitive and specific; automated search produced hardly any false results. To demonstrate the applicability of the adapted workflow to complex samples, 14 wastewater effluent samples collected on seven consecutive days at the local wastewater-treatment plant were analyzed. Of the 88,970 fragment ion mass spectra produced, 8.8% of spectra were successfully assigned to one of the 1040 reference compounds included in the database, and this enabled the identification of 51 compounds representing important illegal drugs, members of various pharmaceutical compound classes, and metabolites thereof. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Should Torsion Balance Technique Continue to be Taught to Pharmacy Students?

PubMed

Bilger, Rhonda; Chereson, Rasma; Salama, Noha Nabil

2017-06-01

Objective. To determine the types of balances used in compounding pharmacies: torsion or digital. Methods. A survey was mailed to the pharmacist-in-charge at 698 pharmacies, representing 47% of the pharmacies in Missouri as of July 2013. The pharmacies were randomly selected and stratified by region into eight regions to ensure a representative sample. Information was gathered regarding the type and use of balances and pharmacists' perspectives on the need to teach torsion balance technique to pharmacy students. Results. The response rate for the survey was 53.3%. Out of the total responses received, those pharmacies having a torsion balance, digital balance or both were 46.8%, 27.4% and 11.8%, respectively. About 68.3% of respondents compound prescriptions. The study showed that 52% of compounding pharmacies use torsion balances in their practice. Of those with a balance in their pharmacy, 65.6% favored continuation of torsion balance instruction. Conclusions. Digital balances have become increasingly popular and have replaced torsion balances in some pharmacies, especially those that compound a significant number of prescriptions. The results of this study indicate that torsion balances remain integral to compounding practice. Therefore, students should continue being taught torsion balance technique at the college.

Frequency of Respiratory Nursing Diagnoses and Accuracy of Clinical Indicators in Preterm Infants.

PubMed

Avena, Marta José; Pedreira, Mavilde da Luz Gonçalves; Bassolli de Oliveira Alves, Lucas; Herdman, T Heather; de Gutiérrez, Maria Gaby Rivero

2018-03-05

To identify the frequency of the nursing diagnoses, ineffective breathing pattern, impaired gas exchange and impaired spontaneous ventilation in newborns; and, to analyze the accuracy of diagnostic indicators identified for each of these diagnoses. This was a cross-sectional study conducted with a nonprobability sample of 92 infants. Data collected were represented by demographic and clinical variables, clinical indicators of the three respiratory nursing diagnoses from NANDA International, and were analyzed according to frequency and agreement between pairs of expert nurses (Kappa). Ineffective breathing pattern was identified in 74.5% of infants; impaired gas exchange was noted in 31.5%; impaired spontaneous ventilation was found in 16.8% of subjects. Use of accessory muscles to breathe showed the highest sensitivity for ineffective breathing pattern; abnormal blood gases had the best predictive value for impaired gas exchange. Use of accessory muscles to breathe had the highest sensitivity for impaired spontaneous ventilation. Ineffective breathing pattern was the most frequently identified; use of accessory muscles, alteration in depth of breathing, abnormal breathing, and dyspnea were the most representative signs/symptoms. Early recognition of respiratory conditions can support safe interventions to ensure appropriate outcomes. © 2018 NANDA International, Inc.

Should Torsion Balance Technique Continue to be Taught to Pharmacy Students?

PubMed Central

Bilger, Rhonda; Chereson, Rasma

2017-01-01

Objective. To determine the types of balances used in compounding pharmacies: torsion or digital. Methods. A survey was mailed to the pharmacist-in-charge at 698 pharmacies, representing 47% of the pharmacies in Missouri as of July 2013. The pharmacies were randomly selected and stratified by region into eight regions to ensure a representative sample. Information was gathered regarding the type and use of balances and pharmacists’ perspectives on the need to teach torsion balance technique to pharmacy students. Results. The response rate for the survey was 53.3%. Out of the total responses received, those pharmacies having a torsion balance, digital balance or both were 46.8%, 27.4% and 11.8%, respectively. About 68.3% of respondents compound prescriptions. The study showed that 52% of compounding pharmacies use torsion balances in their practice. Of those with a balance in their pharmacy, 65.6% favored continuation of torsion balance instruction. Conclusions. Digital balances have become increasingly popular and have replaced torsion balances in some pharmacies, especially those that compound a significant number of prescriptions. The results of this study indicate that torsion balances remain integral to compounding practice. Therefore, students should continue being taught torsion balance technique at the college. PMID:28720913

Attrition analysed in five waves of a longitudinal yearly survey of smokers: findings from the ITC Netherlands survey.

PubMed

Zethof, Dennis; Nagelhout, Gera E; de Rooij, Mark; Driezen, Pete; Fong, Geoffrey T; van den Putte, Bas; Hummel, Karin; de Vries, Hein; Thompson, Mary E; Willemsen, Marc C

2016-08-01

Attrition bias can affect the external validity of findings. This article analyses attrition bias and assesses the effectiveness of replenishment samples on demographic and smoking-related characteristics for the International Tobacco Control Netherlands Survey, a longitudinal survey among smokers. Attrition analyses were conducted for the first five survey waves (2008-12). We assessed, including and excluding replenishment samples, whether the demographic composition of the samples changed between the first and fifth waves. Replenishment samples were tailored to ensure the sample remained representative of the smoking population. We also constructed a multivariable survival model of attrition that included all five waves with replenishment samples. Of the original 1820 respondents recruited in 2008, 46% participated again in 2012. Demographic differences between waves due to attrition were generally small and replenishment samples tended to minimize them further. The multivariable survival analysis revealed that only two of the 10 variables analysed were significant predictors of attrition: a weak effect for gender (men dropped out more often) and weak to moderate effects for age (respondents aged 15-24 years dropped out more than aged 25-39 years, who dropped out more than those aged 40+ years). Weak to moderate attrition effects were found for men and younger age groups. This information could be used to minimize respondent attrition. Our findings suggest that sampling weights and tailored replenishment samples can effectively compensate for attrition effects. This is already being done for the International Tobacco Control Netherlands Survey, including the categories that significantly predicted attrition in this study. © The Author 2016. Published by Oxford University Press on behalf of the European Public Health Association. All rights reserved.

Australian Schizophrenia Research Bank: a database of comprehensive clinical, endophenotypic and genetic data for aetiological studies of schizophrenia.

PubMed

Loughland, Carmel; Draganic, Daren; McCabe, Kathryn; Richards, Jacqueline; Nasir, Aslam; Allen, Joanne; Catts, Stanley; Jablensky, Assen; Henskens, Frans; Michie, Patricia; Mowry, Bryan; Pantelis, Christos; Schall, Ulrich; Scott, Rodney; Tooney, Paul; Carr, Vaughan

2010-11-01

This article describes the establishment of the Australian Schizophrenia Research Bank (ASRB), which operates to collect, store and distribute linked clinical, cognitive, neuroimaging and genetic data from a large sample of people with schizophrenia and healthy controls. Recruitment sources for the schizophrenia sample include a multi-media national advertising campaign, inpatient and community treatment services and non-government support agencies. Healthy controls have been recruited primarily through multi-media advertisements. All participants undergo an extensive diagnostic and family history assessment, neuropsychological evaluation, and blood sample donation for genetic studies. Selected individuals also complete structural MRI scans. Preliminary analyses of 493 schizophrenia cases and 293 healthy controls are reported. Mean age was 39.54 years (SD = 11.1) for the schizophrenia participants and 37.38 years (SD = 13.12) for healthy controls. Compared to the controls, features of the schizophrenia sample included a higher proportion of males (cases 65.9%; controls 46.8%), fewer living in married or de facto relationships (cases 16.1%; controls 53.6%) and fewer years of education (cases 13.05, SD = 2.84; controls 15.14, SD = 3.13), as well as lower current IQ (cases 102.68, SD = 15.51; controls 118.28, SD = 10.18). These and other sample characteristics are compared to those reported in another large Australian sample (i.e. the Low Prevalence Disorders Study), revealing some differences that reflect the different sampling methods of these two studies. The ASRB is a valuable and accessible schizophrenia research facility for use by approved scientific investigators. As recruitment continues, the approach to sampling for both cases and controls will need to be modified to ensure that the ASRB samples are as broadly representative as possible of all cases of schizophrenia and healthy controls.

The flotation tailings of the former Pb-Zn mine of Touiref (NW Tunisia): mineralogy, mine drainage prediction, base-metal speciation assessment and geochemical modeling.

PubMed

Othmani, Mohamed Ali; Souissi, Fouad; Bouzahzah, Hassan; Bussière, Bruno; da Silva, Eduardo Ferreira; Benzaazoua, Mostafa

2015-02-01

The underground extraction of Pb-Zn mineralization in the Touiref area stopped in 1958. A large volume of flotation tailings (more than 500 Mt) containing sulfides were deposited in a tailings impoundment. The goals of this study are to evaluate the neutralization capacity of the unoxidized and oxidized tailings, to assess the speciation of metals between the different components of the tailings material, and to assess the mobility of metals and the secondary minerals' precipitation in pore waters using geochemical modeling. To accomplish these objectives, representative samples from both fresh and oxidized zones were collected along a vertical profile through the tailings pile. Physical, chemical (ICP-MS), and mineralogical characterization (X-ray diffraction (XRD), reflected light microscopy, scanning electron microscope (SEM)) of these samples was performed. Grain size analysis shows that the tailings are dominated by silt- to sand-sized fractions. The microscopic observation highlights the presence of pyrite, marcasite, galena, and sphalerite as primary minerals in a carbonated matrix. The study reveals also the presence of secondary minerals represented by cerussite, smithsonite, anglesite, and Fe oxi-hydroxides as important scavengers for trace elements. The static tests show that the presence of calcite in the tailing samples ensures acid-neutralizing capacity (ANC), which is significantly greater than the acidity potential (PA). The geochemical characterization of the unoxidized samples shows higher Cd, Pb, and Zn concentrations than the oxidized samples containing the highest values for Fe and SO4. Sequential extraction tests show that significant percentages of metals are distributed between the acid-soluble fractions (Cd, Pb, and Zn) and the reducible one (Zn). Pore water analysis indicates that Ca is the dominant cation (8,170 and 6,200 mg L(-1), respectively), whereas sulfate is the principal anion (6,900 and 5,100 mg L(-1), respectively). Saturation index (SI) calculations of minerals in pore water extracted from both the oxidized and unoxidized samples are indicative of gypsum (SI >0) and Fe(III) oxides (SI ≫0) precipitation. The latter controls the Fe concentration in solution.

45 CFR 153.350 - Risk adjustment data validation standards.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 45 Public Welfare 1 2013-10-01 2013-10-01 false Risk adjustment data validation standards. 153.350... validation standards. (a) General requirement. The State, or HHS on behalf of the State, must ensure proper implementation of any risk adjustment software and ensure proper validation of a statistically valid sample of...

45 CFR 153.350 - Risk adjustment data validation standards.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 45 Public Welfare 1 2014-10-01 2014-10-01 false Risk adjustment data validation standards. 153.350... validation standards. (a) General requirement. The State, or HHS on behalf of the State, must ensure proper implementation of any risk adjustment software and ensure proper validation of a statistically valid sample of...

Occurrence of pesticide residues in fruiting vegetables from production farms in south-eastern region of Poland

PubMed

Słowik-Borowiec, Magdalena; Szpyrka, Ewa; Rupar, Julian; Podbielska, Magdalena; Matyaszek, Aneta

Considering the fact that pesticides are commonly used in agriculture, continuous monitoring of these substances in food products is of great significance. Residues of these substances can be present in crops after harvest. The aim of this study was to evaluate presence of pesticide residues in fruiting vegetables from production farms in south-eastern region of Poland in 2012–2015. 138 samples were tested using accredited test methods. The monitoring programme covered determination of 242 pesticides. The tests covered tomato, cucumber and pepper crops. The test results were interpreted in accordance with criteria included in the European Commission recommendations published in the document SANCO/12571/2013 (now superseded by Document SANTE 2015), as well as on a basis of the maximum residue levels in force in the EU Member States. Pesticide residues were found in 47 samples, representing 34% of all tested samples. 17 active substances were found, belonging to fungicides and insecticides. Azoxystrobin (38%), boscalid (28%) and chlorothalonil (21%) were most commonly found in fruiting vegetables testing samples. Non-compliances related to use of plant protection product not authorized for protection of a given crop were observed in 6% of analysed samples. However, pesticide residues of fruiting vegetables in quantities that exceed the maximum residue levels (NDP, ang. MRLs), as well as substances which use for plant protection is forbidden were no found. Crops monitoring is used to determine to what extent such products are contaminated with pesticide residues, and ensures protection of customer health.

41 CFR 102-33.25 - What are our responsibilities under this part?

Code of Federal Regulations, 2010 CFR

2010-07-01

..., GSA) a Senior Aviation Management Official (SAMO), who will be your agency's primary member of the...) Appoint representatives of the agency as members of ICAP subcommittees and working groups; and (h) Ensure...

10 CFR 431.205 - Units to be tested.

Code of Federal Regulations, 2011 CFR

2011-01-01

... to ensure that— (a) Any represented value of estimated input power demand or other measure of energy consumption of a basic model for which consumers would favor lower values shall be no less than the higher of...

40 CFR 258.53 - Ground-water sampling and analysis requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... include consistent sampling and analysis procedures that are designed to ensure monitoring results that... testing period. If a multiple comparisons procedure is used, the Type I experiment wise error rate for...

Mechanical sieve for screening mineral samples

NASA Technical Reports Server (NTRS)

Otto, W. P.

1970-01-01

Mechanical sieve consists of three horizontal screens mounted in a vertical stack. A combination of rotation and tapping produces an even flow across the screens, dislodges trapped particles, an ensures rapid segregation of the sample.

[Family functioning of elderly with depressive symptoms].

PubMed

Souza, Rosely Almeida; Desani da Costa, Gislaine; Yamashita, Cintia Hitomi; Amendola, Fernanda; Gaspar, Jaqueline Correa; Alvarenga, Márcia Regina Martins; Faccenda, Odival; Oliveira, Maria Amélia de Campos

2014-06-01

To classify families of elderly with depressive symptoms regarding their functioning and to ascertain the presence of an association between these symptoms, family functioning and the characteristics of the elderly. This was an observational, analytical, cross-sectional study performed with 33 teams of the Family Health Strategy in Dourados, MS. The sample consisted of 374 elderly divided into two groups (with and without depressive symptoms). The instruments for data collection were a sociodemographic instrument, the GeriatricDepression Scale (15 items) and the Family Apgar. An association was observed between depressive symptoms and family dysfunction, female gender, four or more people living together, and physical inactivity. The functional family may represent effective support for the elderly with depressive symptoms, because it offers a comfortable environment that ensures the well-being of its members. The dysfunctional family can barely provide necessary care for the elderly, which can exacerbate depressive symptoms.

Standardized methods and quality control limits for agar and broth microdilution susceptibility testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum.

PubMed

Waites, Ken B; Duffy, Lynn B; Bébéar, Cécile M; Matlow, Anne; Talkington, Deborah F; Kenny, George E; Totten, Patricia A; Bade, Donald J; Zheng, Xiaotian; Davidson, Maureen K; Shortridge, Virginia D; Watts, Jeffrey L; Brown, Steven D

2012-11-01

An international multilaboratory collaborative study was conducted to develop standard media and consensus methods for the performance and quality control of antimicrobial susceptibility testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum using broth microdilution and agar dilution techniques. A reference strain from the American Type Culture Collection was designated for each species, which was to be used for quality control purposes. Repeat testing of replicate samples of each reference strain by participating laboratories utilizing both methods and different lots of media enabled a 3- to 4-dilution MIC range to be established for drugs in several different classes, including tetracyclines, macrolides, ketolides, lincosamides, and fluoroquinolones. This represents the first multilaboratory collaboration to standardize susceptibility testing methods and to designate quality control parameters to ensure accurate and reliable assay results for mycoplasmas and ureaplasmas that infect humans.

Associations between child disabilities and caregiver discipline and violence in low- and middle-income countries.

PubMed

Hendricks, Charlene; Lansford, Jennifer E; Deater-Deckard, Kirby; Bornstein, Marc H

2014-01-01

Using nationally representative samples of 45,964 two- to nine-year-old children and their primary caregivers in 17 developing countries, this study examined the relations between children's cognitive, language, sensory, and motor disabilities and caregivers' use of discipline and violence. Primary caregivers reported on their child's disabilities and whether they or anyone in their household had used nonviolent discipline, psychological aggression, and physical violence toward the target child and believed that using corporal punishment is necessary. Logistic regression analyses supported the hypothesis that children with disabilities are treated more harshly than children without disabilities. The findings suggest that policies and interventions are needed to work toward the United Nations' goals of ensuring that children with disabilities are protected from abuse and violence. © 2013 The Authors. Child Development © 2013 Society for Research in Child Development, Inc.

Precision wildlife monitoring using unmanned aerial vehicles

NASA Astrophysics Data System (ADS)

Hodgson, Jarrod C.; Baylis, Shane M.; Mott, Rowan; Herrod, Ashley; Clarke, Rohan H.

2016-03-01

Unmanned aerial vehicles (UAVs) represent a new frontier in environmental research. Their use has the potential to revolutionise the field if they prove capable of improving data quality or the ease with which data are collected beyond traditional methods. We apply UAV technology to wildlife monitoring in tropical and polar environments and demonstrate that UAV-derived counts of colony nesting birds are an order of magnitude more precise than traditional ground counts. The increased count precision afforded by UAVs, along with their ability to survey hard-to-reach populations and places, will likely drive many wildlife monitoring projects that rely on population counts to transition from traditional methods to UAV technology. Careful consideration will be required to ensure the coherence of historic data sets with new UAV-derived data and we propose a method for determining the number of duplicated (concurrent UAV and ground counts) sampling points needed to achieve data compatibility.

Racial and ethnic identity in nursing research.

PubMed

Gennaro, Susan; Fantasia, Heidi Collins; Keshinover, Tayra; Garry, David; Wilcox, Wendy; Uppal, Elyse

2013-01-01

Nurse researchers need to be able to identify the race and ethnicity of participants in their studies for several reasons including addressing health disparities, ensuring adequate representation from under-represented minorities, and making sure other nurses can understand how findings may or may not pertain to their own patient population. However, obtaining accurate information about race and ethnicity requires careful attention to norms of study participants. Race and ethnicity are not always viewed as 2 separate constructs and the definition of both changes over time. In fact, a random sample of 100 patients in 1 hospital found an 11% discrepancy between patients' self-identification of race using 2 different methodologies of self-identification. To optimize accuracy of self-identification of race and ethnicity, this paper discusses techniques learned in practice and in the literature for improving self-identification of these 2 constructs. Copyright © 2013 Elsevier Inc. All rights reserved.

NHEXAS PHASE I MARYLAND STUDY--STANDARD OPERATING PROCEDURE FOR DUPLICATE SAMPLING (F12)

EPA Science Inventory

This SOP is designed to provide both general and specific guidance for the collection of duplicate samples by Harvard School of Public Health/Emory University for the NHEXAS project. The purpose of the duplicate sampling was to ensure that the value obtained for a sample was ind...

A study to examine the relationship between metritis severity and depletion of oxytetracycline in plasma and milk after intrauterine infusion.

PubMed

Gorden, P J; Ydstie, J A; Kleinhenz, M D; Wulf, L W; Gehring, R; Lee, C J; Wang, C; Coetzee, J F

2016-10-01

Metritis is a frequent problem in postpartum dairy cows. Intrauterine therapy with the antimicrobial oxytetracycline (OTC) is often used, although this therapy has not been shown to be superior to systemic therapy. The objectives of this study were to (1) determine the plasma and milk concentrations of OTC following intrauterine infusion in postpartum dairy cows with varying degrees of metritis severity; (2) determine the depletion time of OTC in an attempt to provide veterinarians withdrawal guidelines, should they use this therapy; and (3) correlate metritis severity scores with OTC concentrations in plasma and milk. Our hypothesis was that cows with more severe metritis would have higher OTC concentrations in milk following intrauterine therapy. Thirty-two cows were selected to participate in the study after farm personnel had determined that they had metritis based on evaluation of vaginal discharge between 4 and 14 DIM, in accordance with the farm's treatment protocols. Metritis scores (1-4) were assigned based on a published scheme: 1 represented yellow-to-orange thick discharge or translucent mucus with no fetid smell; 2 represented blood-tinged vaginal mucus, slightly watery, with little or no fetid smell; 3 represented red to red/brown watery discharge with moderate fetid smell; and 4 represented red to red/brown watery discharge containing pieces of placenta and an intense fetid smell. Trial cows received a single treatment of 4g of OTC (approximately 6.7mg/kg) via intrauterine infusion. Blood samples were collected over 96h, and milk samples were collected before intrauterine therapy and 3 times a day for 4 d following infusion. Following treatment, OTC rapidly diffused to plasma and subsequently to milk. Maximum OTC concentrations in plasma and milk occurred within the first 24h following intrauterine infusion, and 25 of the 32 cows had detectable OTC concentrations in milk at 4 d after intrauterine infusion. Cows with clinical metritis (metritis severity scores of 3 or 4) at the initiation of treatment were significantly and positively correlated with higher milk OTC concentrations at the second [time (T)9 h; r=0.43], fourth (T25 h; r=0.42), and fifth milking following treatment (T33 h; r=0.38) compared with cows with normal vaginal discharge. We also observed a positive correlation between initial metritis score and milk maximum concentration (r=0.36) and milk area under the concentration curve (r=0.36). Given that intrauterine administration of OTC is an extra-label therapy, dairy producers should consult with their veterinarian to ensure that milk is being tested at or below the established tolerance for OTC. This will ensure that violative drug residues do not enter the human food supply. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

LLNL Genomic Assessment: Viral and Bacterial Sequencing Needs for TMTI, Task 1.4.2 Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Slezak, T; Borucki, M; Lam, M

Good progress has been made on both bacterial and viral sequencing by the TMTI centers. While access to appropriate samples is a limiting factor to throughput, excellent progress has been made with respect to getting agreements in place with key sources of relevant materials. Sharing of sequenced genomes funded by TMTI has been extremely limited to date. The April 2010 exercise should force a resolution to this, but additional managerial pressures may be needed to ensure that rapid sharing of TMTI-funded sequencing occurs, regardless of collaborator constraints concerning ultimate publication(s). Policies to permit TMTI-internal rapid sharing of sequenced genomes shouldmore » be written into all TMTI agreements with collaborators now being negotiated. TMTI needs to establish a Web-based system for tracking samples destined for sequencing. This includes metadata on sample origins and contributor, information on sample shipment/receipt, prioritization by TMTI, assignment to one or more sequencing centers (including possible TMTI-sponsored sequencing at a contributor site), and status history of the sample sequencing effort. While this system could be a component of the AFRL system, it is not part of any current development effort. Policy and standardized procedures are needed to ensure appropriate verification of all TMTI samples prior to the investment in sequencing. PCR, arrays, and classical biochemical tests are examples of potential verification methods. Verification is needed to detect miss-labeled, degraded, mixed or contaminated samples. Regular QC exercises are needed to ensure that the TMTI-funded centers are meeting all standards for producing quality genomic sequence data.« less

Microbiological assessment of honey in México.

PubMed

Vázquez-Quiñones, Carlos Ramón; Moreno-Terrazas, Rúben; Natividad-Bonifacio, Iván; Quiñones-Ramírez, Elsa Irma; Vázquez-Salinas, Carlos

Honey is a product used as a natural sweetener and in several regions of Mexico and other countries it is also used as a therapeutic agent. Microbiological contamination of honey can occur during its extraction and handling. Due to the use and consumption of honey we highlighted here the importance of the assessment of its microbiological quality. One thousand nine hundred twenty samples obtained from 8 honey-producing states from Mexico were analyzed. From these samples, 40.5% (777/1920) did not comply with the NMX-036-NORMEX-2006 specification. Forty five percent (777/1920) of the samples did not comply with the mesophilic aerobic microorganism specification, neither did 17% (327/1920) of the samples with the specification for molds and 18.1% (348/1920) with the specification for yeasts. With regard to coliform bacteria, the samples contained less than 3 NMP/g. Two percent of the samples contained lactic acid bacteria (LAB), Clostridium perfringens was observed in amounts of more than 100CFU/g. None of the samples from the different states contained more than 100CFU/g of Staphylococcus aureus; Salmonella spp. was absent in all samples. It is important to avoid contamination sources and implement good hygienic practices in order to maintain and improve the quality of Mexican honeys since a large percentage of them are intended for export. If these honeys are intended for therapeutic use, it is necessary to ensure that they comply with all quality parameters and to apply specific treatments that guarantee the removal of any pathogen that may represent a risk to the patients's health. Copyright © 2017 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters

PubMed Central

Aagaard, Kjersti; Petrosino, Joseph; Keitel, Wendy; Watson, Mark; Katancik, James; Garcia, Nathalia; Patel, Shital; Cutting, Mary; Madden, Tessa; Hamilton, Holli; Harris, Emily; Gevers, Dirk; Simone, Gina; McInnes, Pamela; Versalovic, James

2013-01-01

The Human Microbiome Project used rigorous good clinical practice standards to complete comprehensive body site sampling in healthy 18- to 40-yr-old adults, creating an unparalleled reference set of microbiome specimens. To ensure that specimens represented minimally perturbed microbiomes, we first screened potential participants using exclusion criteria based on health history, including the presence of systemic diseases (e.g., hypertension, cancer, or immunodeficiency or autoimmune disorders), use of potential immunomodulators, and recent use of antibiotics or probiotics. Subsequent physical examinations excluded individuals based on body mass index (BMI), cutaneous lesions, and oral health. We screened 554 individuals to enroll 300 (149 men and 151 women, mean age 26 yr, mean BMI 24 kg/m2, 20.0% racial minority, and 10.7% Hispanic). We obtained specimens from the oral cavity, nares, skin, gastrointestinal tract, and vagina (15 specimens from men and 18 from women). The study evaluated longitudinal changes in an individual's microbiome by sampling 279 participants twice (mean 212 d after the first sampling; range 30-359 d) and 100 individuals 3 times (mean 72 d after the second sampling; range 30-224 d). This sampling strategy yielded 11,174 primary specimens, from which 12,479 DNA samples were submitted to 4 centers for metagenomic sequencing. Our clinical design and well-defined reference cohort has laid a foundation for microbiome research.—Aagaard, K., Petrosino, J., Keitel, W., Watson, M., Katancik, J., Garcia, N., Patel, S., Cutting, M., Madden, T., Hamilton, H., Harris, E., Gevers, D., Simone, G., McInnes, P., Versalovic, J. The Human Microbiome Project strategy for comprehensive sampling of the human microbiome and why it matters. PMID:23165986

Replicating studies in which samples of participants respond to samples of stimuli.

PubMed

Westfall, Jacob; Judd, Charles M; Kenny, David A

2015-05-01

In a direct replication, the typical goal is to reproduce a prior experimental result with a new but comparable sample of participants in a high-powered replication study. Often in psychology, the research to be replicated involves a sample of participants responding to a sample of stimuli. In replicating such studies, we argue that the same criteria should be used in sampling stimuli as are used in sampling participants. Namely, a new but comparable sample of stimuli should be used to ensure that the original results are not due to idiosyncrasies of the original stimulus sample, and the stimulus sample must often be enlarged to ensure high statistical power. In support of the latter point, we discuss the fact that in experiments involving samples of stimuli, statistical power typically does not approach 1 as the number of participants goes to infinity. As an example of the importance of sampling new stimuli, we discuss the bygone literature on the risky shift phenomenon, which was almost entirely based on a single stimulus sample that was later discovered to be highly unrepresentative. We discuss the use of both resampled and expanded stimulus sets, that is, stimulus samples that include the original stimuli plus new stimuli. © The Author(s) 2015.

Proceedings of Bus Summit 2000 : ensuring a healthy U.S. bus industry

DOT National Transportation Integrated Search

2000-10-01

The Federal Transit Administration (FTA) Bus Industry Summit was held October 18-19, 2000, in Washington, DC. The Summit offered an opportunity for representatives from the transit industry, including transit providers, vehicle manufacturers, consult...

40 CFR Appendix I to Part 261 - Representative Sampling Methods

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Representative Sampling Methods I...—Representative Sampling Methods The methods and equipment used for sampling waste materials will vary with the form and consistency of the waste materials to be sampled. Samples collected using the sampling...

40 CFR Appendix I to Part 261 - Representative Sampling Methods

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Representative Sampling Methods I...—Representative Sampling Methods The methods and equipment used for sampling waste materials will vary with the form and consistency of the waste materials to be sampled. Samples collected using the sampling...

hEIDI: An Intuitive Application Tool To Organize and Treat Large-Scale Proteomics Data.

PubMed

Hesse, Anne-Marie; Dupierris, Véronique; Adam, Claire; Court, Magali; Barthe, Damien; Emadali, Anouk; Masselon, Christophe; Ferro, Myriam; Bruley, Christophe

2016-10-07

Advances in high-throughput proteomics have led to a rapid increase in the number, size, and complexity of the associated data sets. Managing and extracting reliable information from such large series of data sets require the use of dedicated software organized in a consistent pipeline to reduce, validate, exploit, and ultimately export data. The compilation of multiple mass-spectrometry-based identification and quantification results obtained in the context of a large-scale project represents a real challenge for developers of bioinformatics solutions. In response to this challenge, we developed a dedicated software suite called hEIDI to manage and combine both identifications and semiquantitative data related to multiple LC-MS/MS analyses. This paper describes how, through a user-friendly interface, hEIDI can be used to compile analyses and retrieve lists of nonredundant protein groups. Moreover, hEIDI allows direct comparison of series of analyses, on the basis of protein groups, while ensuring consistent protein inference and also computing spectral counts. hEIDI ensures that validated results are compliant with MIAPE guidelines as all information related to samples and results is stored in appropriate databases. Thanks to the database structure, validated results generated within hEIDI can be easily exported in the PRIDE XML format for subsequent publication. hEIDI can be downloaded from http://biodev.extra.cea.fr/docs/heidi .

Historically Black Colleges and Universities Historic Building Restoration and Preservation Act. Report of the House Committee on Resources To Accompany H.R. 1179. House of Representatives, 104th Congress, Second Session.

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. House Committee on Resources.

This report of the Committee on Resources recommends House of Representatives approval of (H.R. 1179), the Historically Black Colleges and Universities Historic Building Restoration and Preservation Act, as amended, to ensure the preservation of buildings eligible for funding under the Historic Preservation Act on the campuses of historically…

21 CFR 111.80 - What representative samples must you collect?

Code of Federal Regulations, 2010 CFR

2010-04-01

... Process Control System § 111.80 What representative samples must you collect? The representative samples... unique lot within each unique shipment); (b) Representative samples of in-process materials for each manufactured batch at points, steps, or stages, in the manufacturing process as specified in the master...

Precision Efficacy Analysis for Regression.

ERIC Educational Resources Information Center

Brooks, Gordon P.

When multiple linear regression is used to develop a prediction model, sample size must be large enough to ensure stable coefficients. If the derivation sample size is inadequate, the model may not predict well for future subjects. The precision efficacy analysis for regression (PEAR) method uses a cross- validity approach to select sample sizes…

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR CUSTODY OF FIELD SAMPLES (UA-C-1.0)

EPA Science Inventory

The purpose of this SOP is to outline sample custody guidelines used by the NHEXAS Arizona research project. This procedure was followed to maintain sample integrity and ensure consistent data retrieval, transport, storage, shipment, and analysis during the Arizona NHEXAS projec...

Trends and Future Challenges in Sampling the Deep Terrestrial Biosphere

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilkins, Michael J.; Daly, Rebecca; Mouser, Paula J.

2014-09-12

Research in the deep terrestrial biosphere is driven by interest in novel biodiversity and metabolisms, biogeochemical cycling, and the impact of human activities on this ecosystem. As this interest continues to grow, it is important to ensure that when subsurface investigations are proposed, materials recovered from the subsurface are sampled and preserved in an appropriate manner to limit contamination and ensure preservation of accurate microbial, geochemical, and mineralogical signatures. On February 20th, 2014, a workshop on “Trends and Future Challenges in Sampling The Deep Subsurface” was coordinated in Columbus, Ohio by The Ohio State University and West Virginia University faculty,more » and sponsored by The Ohio State University and the Sloan Foundation’s Deep Carbon Observatory. The workshop aims were to identify and develop best practices for the collection, preservation, and analysis of terrestrial deep rock samples. This document summarizes the information shared during this workshop.« less

Trends and future challenges in sampling the deep terrestrial biosphere.

PubMed

Wilkins, Michael J; Daly, Rebecca A; Mouser, Paula J; Trexler, Ryan; Sharma, Shihka; Cole, David R; Wrighton, Kelly C; Biddle, Jennifer F; Denis, Elizabeth H; Fredrickson, Jim K; Kieft, Thomas L; Onstott, Tullis C; Peterson, Lee; Pfiffner, Susan M; Phelps, Tommy J; Schrenk, Matthew O

2014-01-01

Research in the deep terrestrial biosphere is driven by interest in novel biodiversity and metabolisms, biogeochemical cycling, and the impact of human activities on this ecosystem. As this interest continues to grow, it is important to ensure that when subsurface investigations are proposed, materials recovered from the subsurface are sampled and preserved in an appropriate manner to limit contamination and ensure preservation of accurate microbial, geochemical, and mineralogical signatures. On February 20th, 2014, a workshop on "Trends and Future Challenges in Sampling The Deep Subsurface" was coordinated in Columbus, Ohio by The Ohio State University and West Virginia University faculty, and sponsored by The Ohio State University and the Sloan Foundation's Deep Carbon Observatory. The workshop aims were to identify and develop best practices for the collection, preservation, and analysis of terrestrial deep rock samples. This document summarizes the information shared during this workshop.

DEVELOPMENTS IN THE APPLICATION OF IMPACT ASSESSMENT METHODOLOGIES FOR POLLUTION PREVENTION

EPA Science Inventory

Pollution prevention requires the assessment of various multimedia environmental impacts to ensure that the alternative selected most closely represents the environmental goals and priorities of the facility. While some facility's environmental policies are easy to assess, others...

32 CFR 634.24 - Traffic planning and codes.

Code of Federal Regulations, 2010 CFR

2010-07-01

... enforcement officer, engineer, safety officer, and other concerned staff agencies. Highway engineering representatives from adjacent civil communities must be consulted to ensure the installation plan is compatible... and minor routes, location of traffic control devices, and conditions requiring engineering or...

Association between depression and tinnitus in a nationally representative sample of US older adults.

PubMed

Loprinzi, Paul D; Maskalick, Shawn; Brown, Kent; Gilham, Ben

2013-01-01

Few population-based studies examining the association between tinnitus and depression among older adults have been conducted. Therefore, the purpose of this study was to examine the association between tinnitus and depression among a nationally representative sample of US older adults. Data from the 2005-2006 National Health and Nutrition Examination Survey was used. 696 older adults (70-85 yr) completed questionnaires on tinnitus and depression, with depression assessed using the Patient Health Questionnaire-9. After controlling for firearm use, age, gender, race-ethnicity, cardiovascular/stroke history, diabetes, smoking status, body mass index, physical activity, noise exposure and elevated blood pressure, there was a significant positive association (beta coefficient: 1.28, 95% CI: 0.26-2.29, p = 0.01) between depression and tinnitus being at least a moderate problem, suggesting that those who perceived their tinnitus to be a moderate problem were more likely to be depressed than those perceiving it to be a small or no problem. Additionally, after adjustments, those who were bothered by tinnitus when going to bed were 3.06 times more likely to be depressed than those who were not bothered by tinnitus when going to bed (OR = 2.44, 95% CI: 1.03-5.76, p = 0.04). These findings suggest that individuals who perceive their tinnitus to be a problem or have problems with tinnitus when going to bed may be in need of intervention to prevent or reduce their depression symptoms so as to ensure that other areas of their life are not negatively influenced.

Accident Proneness in Children and Adolescents Affected by ADHD and the Impact of Medication.

PubMed

Lange, Hannah; Buse, Judith; Bender, Stephan; Siegert, Joachim; Knopf, Hildtraud; Roessner, Veit

2016-06-01

This study aims to ascertain once and for all whether children and adolescents affected by ADHD show a higher risk for accidents, as well as investigating a possible association between the administration of ADHD-specific medication and the occurrence of accidents. Two exceptionally large sets of data were implemented in this analysis. Participants included children and adolescents representative of the entire German population. Data for Survey 1 was collected through extensive administration of questionnaires. Data for Survey 2 stemmed from the records of a leading German health insurance company. In terms of statistical analysis, chi-square tests as well as logistic regression analyses were applied and odds ratios (ORs) were determined. Innovative results are presented showing a significantly higher likelihood for ADHD-affected youngsters to be involved in accidents compared with their nonaffected counterparts (Survey 1: OR = 1.60; Survey 2: OR = 1.89) but lacking an overall significant influence of medication regarding the occurrence of accidents (Survey 1: OR = 1.28; Survey 2: OR = 0.97). Frequency of accidents could be predicted by ADHD, gender, and age in both samples. Medication intake served as a weak predictor only in Survey 2. It has been determined in two representative and independent German samples that youngsters with ADHD are at a significantly higher risk of being involved in accidents. In the future, this should always be considered when setting up a treatment plan to ensure a safer and healthier coming of age without relying solely on specific effects of medication. (J. of Att. Dis. 2016; 20(6) 501-509). © The Author(s) 2014.

Generalizability of Clinical Trial Results for Adolescent Major Depressive Disorder.

PubMed

Blanco, Carlos; Hoertel, Nicolas; Franco, Silvia; Olfson, Mark; He, Jian-Ping; López, Saioa; González-Pinto, Ana; Limosin, Frédéric; Merikangas, Kathleen R

2017-12-01

Although there have been a number of clinical trials evaluating treatments for adolescents with major depressive disorder (MDD), the generalizability of those trials to samples of depressed adolescents who present for routine clinical care is unknown. Examining the generalizability of clinical trials of pharmacological and psychotherapy interventions for adolescent depression can help administrators and frontline practitioners determine the relevance of these studies for their patients and may also guide eligibility criteria for future clinical trials in this clinical population. Data on nationally representative adolescents were derived from the National Comorbidity Survey: Adolescent Supplement. To assess the generalizability of adolescent clinical trials for MDD, we applied a standard set of eligibility criteria representative of clinical trials to all adolescents in the National Comorbidity Survey: Adolescent Supplement with a Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition diagnosis of MDD ( N = 592). From the overall MDD sample, 61.9% would have been excluded from a typical pharmacological trial, whereas 42.2% would have been excluded from a psychotherapy trial. Among those who sought treatment ( n = 412), the corresponding exclusion rates were 72.7% for a pharmacological trial and 52.2% for a psychotherapy trial. The criterion leading to the largest number of exclusions was "significant risk of suicide" in both pharmacological and psychotherapy trials. Pharmacological and, to a lesser extent, psychotherapy clinical trials likely exclude most adolescents with MDD. Careful consideration should be given to balancing eligibility criteria and internal validity with applicability in routine clinical care while ensuring patient safety. Copyright © 2017 by the American Academy of Pediatrics.

Integration of miRNA and Protein Profiling Reveals Coordinated Neuroadaptations in the Alcohol-Dependent Mouse Brain

PubMed Central

Gorini, Giorgio; Nunez, Yury O.; Mayfield, R. Dayne

2013-01-01

The molecular mechanisms underlying alcohol dependence involve different neurochemical systems and are brain region-dependent. Chronic Intermittent Ethanol (CIE) procedure, combined with a Two-Bottle Choice voluntary drinking paradigm, represents one of the best available animal models for alcohol dependence and relapse drinking. MicroRNAs, master regulators of the cellular transcriptome and proteome, can regulate their targets in a cooperative, combinatorial fashion, ensuring fine tuning and control over a large number of cellular functions. We analyzed cortex and midbrain microRNA expression levels using an integrative approach to combine and relate data to previous protein profiling from the same CIE-subjected samples, and examined the significance of the data in terms of relative contribution to alcohol consumption and dependence. MicroRNA levels were significantly altered in CIE-exposed dependent mice compared with their non-dependent controls. More importantly, our integrative analysis identified modules of coexpressed microRNAs that were highly correlated with CIE effects and predicted target genes encoding differentially expressed proteins. Coexpressed CIE-relevant proteins, in turn, were often negatively correlated with specific microRNA modules. Our results provide evidence that microRNA-orchestrated translational imbalances are driving the behavioral transition from alcohol consumption to dependence. This study represents the first attempt to combine ex vivo microRNA and protein expression on a global scale from the same mammalian brain samples. The integrative systems approach used here will improve our understanding of brain adaptive changes in response to drug abuse and suggests the potential therapeutic use of microRNAs as tools to prevent or compensate multiple neuroadaptations underlying addictive behavior. PMID:24358208

Methodology of Global Adult Tobacco Survey (GATS), Malaysia, 2011

PubMed Central

Omar, Azahadi; Yusoff, Muhammad Fadhli Mohd; Hiong, Tee Guat; Aris, Tahir; Morton, Jeremy; Pujari, Sameer

2015-01-01

Introduction Malaysia participated in the second phase of the Global Adult Tobacco Survey (GATS) in 2011. GATS, a new component of the Global Tobacco Surveillance System, is a nationally representative household survey of adults 15 years old or above. The objectives of GATS Malaysia were to (i) systematically monitor tobacco use among adults and track key indicators of tobacco control and (ii) track the implementation of some of the Framework Convention of Tobacco Control (FCTC)-recommended demand related policies. Methods GATS Malaysia 2011 was a nationwide cross-sectional survey using multistage stratified sampling to select 5112 nationally representative households. One individual aged 15 years or older was randomly chosen from each selected household and interviewed using handheld device. GATS Core Questionnaire with optional questions was pre-tested and uploaded into handheld devices after repeated quality control processes. Data collectors were trained through a centralized training. Manuals and picture book were prepared to aid in the training of data collectors and during data collection. Field-level data were aggregated on a daily basis and analysed twice a week. Quality controls were instituted to ensure collection of high quality data. Sample weighting and analysis were conducted with the assistance of researchers from the Centers for Disease Control and Prevention, Atlanta, USA Results GATS Malaysia received a total response rate of 85.3% from 5112 adults surveyed. Majority of the respondents were 25–44 years old and Malays. Conclusions The robust methodology used in the GATS Malaysia provides national estimates for tobacco used classified by socio-demographic characteristics and reliable data on various dimensions of tobacco control. PMID:26451348

Soil Carbon Variability and Change Detection in the Forest Inventory Analysis Database of the United States

NASA Astrophysics Data System (ADS)

Wu, A. M.; Nater, E. A.; Dalzell, B. J.; Perry, C. H.

2014-12-01

The USDA Forest Service's Forest Inventory Analysis (FIA) program is a national effort assessing current forest resources to ensure sustainable management practices, to assist planning activities, and to report critical status and trends. For example, estimates of carbon stocks and stock change in FIA are reported as the official United States submission to the United Nations Framework Convention on Climate Change. While the main effort in FIA has been focused on aboveground biomass, soil is a critical component of this system. FIA sampled forest soils in the early 2000s and has remeasurement now underway. However, soil sampling is repeated on a 10-year interval (or longer), and it is uncertain what magnitude of changes in soil organic carbon (SOC) may be detectable with the current sampling protocol. We aim to identify the sensitivity and variability of SOC in the FIA database, and to determine the amount of SOC change that can be detected with the current sampling scheme. For this analysis, we attempt to answer the following questions: 1) What is the sensitivity (power) of SOC data in the current FIA database? 2) How does the minimum detectable change in forest SOC respond to changes in sampling intervals and/or sample point density? Soil samples in the FIA database represent 0-10 cm and 10-20 cm depth increments with a 10-year sampling interval. We are investigating the variability of SOC and its change over time for composite soil data in each FIA region (Pacific Northwest, Interior West, Northern, and Southern). To guide future sampling efforts, we are employing statistical power analysis to examine the minimum detectable change in SOC storage. We are also investigating the sensitivity of SOC storage changes under various scenarios of sample size and/or sample frequency. This research will inform the design of future FIA soil sampling schemes and improve the information available to international policy makers, university and industry partners, and the public.

Unsupervised learning of discriminative edge measures for vehicle matching between nonoverlapping cameras.

PubMed

Shan, Ying; Sawhney, Harpreet S; Kumar, Rakesh

2008-04-01

This paper proposes a novel unsupervised algorithm learning discriminative features in the context of matching road vehicles between two non-overlapping cameras. The matching problem is formulated as a same-different classification problem, which aims to compute the probability of vehicle images from two distinct cameras being from the same vehicle or different vehicle(s). We employ a novel measurement vector that consists of three independent edge-based measures and their associated robust measures computed from a pair of aligned vehicle edge maps. The weight of each measure is determined by an unsupervised learning algorithm that optimally separates the same-different classes in the combined measurement space. This is achieved with a weak classification algorithm that automatically collects representative samples from same-different classes, followed by a more discriminative classifier based on Fisher' s Linear Discriminants and Gibbs Sampling. The robustness of the match measures and the use of unsupervised discriminant analysis in the classification ensures that the proposed method performs consistently in the presence of missing/false features, temporally and spatially changing illumination conditions, and systematic misalignment caused by different camera configurations. Extensive experiments based on real data of over 200 vehicles at different times of day demonstrate promising results.

The Influence of Maternal and Household Resources, and Parental Psychosocial Child Stimulation on Early Childhood Development: A Cross-Sectional Study of Children 36–59 Months in Honduras

PubMed Central

Urke, Helga Bjørnøy; Contreras, Mariela; Matanda, Dennis Juma

2018-01-01

Optimal early childhood development (ECD) is currently jeopardized for more than 250 million children under five in low- and middle-income countries. The Sustainable Development Goals has called for a renewed emphasis on children’s wellbeing, encompassing a holistic approach that ensures nurturing care to facilitate optimal child development. In vulnerable contexts, the extent of a family’s available resources can influence a child’s potential of reaching its optimal development. Few studies have examined these relationships in low- and middle-income countries using nationally representative samples. The present paper explored the relationships between maternal and paternal psychosocial stimulation of the child as well as maternal and household resources and ECD among 2729 children 36–59 months old in Honduras. Data from the Demographic and Health Surveys conducted in 2011–2012 was used. Adjusted logistic regression analyses showed that maternal psychosocial stimulation was positively and significantly associated with ECD in the full, rural, and lowest wealth quintile samples. These findings underscore the importance of maternal engagement in facilitating ECD but also highlight the role of context when designing tailored interventions to improve ECD. PMID:29735895

Classification of stellar spectra with SVM based on within-class scatter and between-class scatter

NASA Astrophysics Data System (ADS)

Liu, Zhong-bao; Zhou, Fang-xiao; Qin, Zhen-tao; Luo, Xue-gang; Zhang, Jing

2018-07-01

Support Vector Machine (SVM) is a popular data mining technique, and it has been widely applied in astronomical tasks, especially in stellar spectra classification. Since SVM doesn't take the data distribution into consideration, and therefore, its classification efficiencies can't be greatly improved. Meanwhile, SVM ignores the internal information of the training dataset, such as the within-class structure and between-class structure. In view of this, we propose a new classification algorithm-SVM based on Within-Class Scatter and Between-Class Scatter (WBS-SVM) in this paper. WBS-SVM tries to find an optimal hyperplane to separate two classes. The difference is that it incorporates minimum within-class scatter and maximum between-class scatter in Linear Discriminant Analysis (LDA) into SVM. These two scatters represent the distributions of the training dataset, and the optimization of WBS-SVM ensures the samples in the same class are as close as possible and the samples in different classes are as far as possible. Experiments on the K-, F-, G-type stellar spectra from Sloan Digital Sky Survey (SDSS), Data Release 8 show that our proposed WBS-SVM can greatly improve the classification accuracies.

Feline fecal virome reveals novel and prevalent enteric viruses

PubMed Central

Ng, Terry Fei Fan; Mesquita, João Rodrigo; Nascimento, Maria São José; Kondov, Nikola O.; Wong, Walt; Reuter, Gábor; Knowles, Nick J.; Vega, Everardo; Esona, Mathew D.; Deng, Xutao; Vinjé, Jan; Delwart, Eric

2014-01-01

Humans keep more than 80 million cats worldwide, ensuring frequent contacts with their viruses. Despite such interactions the enteric virome of cats remains poorly understood. We analyzed a fecal sample from a single healthy cat from Portugal using viral metagenomics and detected five eukaryotic viral genomes. These viruses included a novel picornavirus (proposed genus “Sakobuvirus”) and bocavirus (feline bocavirus 2), a variant of feline astrovirus 2 and sequence fragments of a highly divergent feline rotavirus and picobirnavirus. Feline sakobuvirus A represents the prototype species of a proposed new genus in the Picornaviridae family, distantly related to human salivirus and kobuvirus. Feline astroviruses (mamastrovirus 2) are the closest relatives of the classic human astroviruses (mamastrovirus 1), suggestive of past cross-species transmission. Presence of these viruses by PCR among Portuguese cats was detected in 13% (rotavirus), 7% (astrovirus), 6% (bocavirus), 4% (sakobuvirus), and 4% (picobirnavirus) of 55 feline fecal samples. Co-infections were frequent with 40% (4/10) of cats shedding more than one of these viruses. Our study provides an initial unbiased description of the feline fecal virome indicating a high level of asymptomatic infections. Availability of the genome sequences of these viruses will facilitate future tropism and disease association studies. PMID:24793097

Colorado River fish monitoring in Grand Canyon, Arizona; 2000 to 2009 summary

USGS Publications Warehouse

Makinster, Andrew S.; Persons, William R.; Avery, Luke A.; Bunch, Aaron J.

2010-01-01

Long-term fish monitoring in the Colorado River below Glen Canyon Dam is an essential component of the Glen Canyon Dam Adaptive Management Program (GCDAMP). The GCDAMP is a federally authorized initiative to ensure that the primary mandate of the Grand Canyon Protection Act of 1992 to protect resources downstream from Glen Canyon Dam is met. The U.S. Geological Survey's Grand Canyon Monitoring and Research Center is responsible for the program's long-term fish monitoring, which is implemented in cooperation with the Arizona Game and Fish Department, U.S. Fish and Wildlife Service, SWCA Environmental Consultants, and others. Electrofishing and tagging protocols have been developed and implemented for standardized annual monitoring of Colorado River fishes since 2000. In 2009, sampling occurred throughout the river between Lees Ferry and Lake Mead for 38 nights over two trips. During the two trips, scientists captured 6,826 fish representing 11 species. Based on catch-per-unit-effort, salmonids (for example, rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta)) increased eightfold between 2006 and 2009. Flannelmouth sucker (Catostomus latipinnis) catch rates were twice as high in 2009 as in 2006. Humpback chub (Gila cypha) catches were low throughout the 10-year sampling period.

Australian health-related quality of life population norms derived from the SF-6D.

PubMed

Norman, Richard; Church, Jody; van den Berg, Bernard; Goodall, Stephen

2013-02-01

To investigate population health-related quality of life norms in an Australian general sample by age, gender, BMI, education and socioeconomic status. The SF-36 was included in the 2009/10 wave of the Household, Income and Labour Dynamics in Australia (HILDA) survey (n=17,630 individuals across 7,234 households), and converted into SF-6D utility scores. Trends across the various population subgroups were investigated employing population weights to ensure a balanced panel, and were all sub-stratified by gender. SF-6D scores decline with age beyond 40 years, with decreasing education and by higher levels of socioeconomic disadvantage. Scores were also lower at very low and very high BMI levels. Males reported higher SF-6D scores than females across most analyses. This study reports Australian population utility data measured using the SF-6D, based on a national representative sample. These results can be used in a range of policy settings such as cost-utility analysis or exploration of health-related inequality. In general, the patterns are similar to those reported using other multi-attribute utility instruments and in different countries. © 2013 The Authors. ANZJPH © 2013 Public Health Association of Australia.

Methods for collection and analysis of geopressured geothermal and oil field waters

USGS Publications Warehouse

Lico, Michael S.; Kharaka, Yousif K.; Carothers, William W.; Wright, Victoria A.

1982-01-01

Present methods are described for the collection, preservation, and chemical analysis of waters produced from geopressured geothermal and petroleum wells. Detailed procedures for collection include precautions and equipment necessary to ensure that the sample is representative of the water produced. Procedures for sample preservation include filtration, acidification, dilution for silica, methyl isobutyl ketone (MIBK) extraction of aluminum, addition of potassium permanganate to preserve mercury, and precipitation of carbonate species as strontium carbonate for stable carbon isotopes and total dissolved carbonate analysis. Characteristics determined at the well site are sulfide, pH, ammonia, and conductivity. Laboratory procedures are given for the analysis of lithium, sodium, potassium, rubidium, cesium, magnesium, calcium, strontium, barium, iron, manganese, zinc, lead, aluminum, .and mercury by atomic absorption and flame emission spectroscopy. Chloride is determined by silver nitrate titration and fluoride by ion-specific electrode. Bromide and iodide concentrations are determined by the hypochlorite oxidation method. Sulfate is analyzed by titration using barium chloride with thorin indicator after pretreatment with alumina. Boron and silica are determined colorimetrically by the carmine and molybdate-blue methods, respectively. Aliphatic acid anions (C2 through C5) are determined by gas chromatography after separation and concentration in a chloroform-butanol mixture.

Socioeconomic Factors Influence Physical Activity and Sport in Quebec Schools.

PubMed

Morin, Pascale; Lebel, Alexandre; Robitaille, Éric; Bisset, Sherri

2016-11-01

School environments providing a wide selection of physical activities and sufficient facilities are both essential and formative to ensure young people adopt active lifestyles. We describe the association between school opportunities for physical activity and socioeconomic factors measured by low-income cutoff index, school size (number of students), and neighborhood population density. A cross-sectional survey using a 2-stage stratified sampling method built a representative sample of 143 French-speaking public schools in Quebec, Canada. Self-administered questionnaires collected data describing the physical activities offered and schools' sports facilities. Descriptive and bivariate analyses were performed separately for primary and secondary schools. In primary schools, school size was positively associated with more intramural and extracurricular activities, more diverse interior facilities, and activities promoting active transportation. Low-income primary schools were more likely to offer a single gym. Low-income secondary schools offered lower diversity of intramural activities and fewer exterior sporting facilities. High-income secondary schools with a large school size provided a greater number of opportunities, larger infrastructures, and a wider selection of physical activities than smaller low-income schools. Results reveal an overall positive association between school availability of physical and sport activity and socioeconomic factors. © 2016, American School Health Association.

An Interpretative Study on Nurses' Perspectives of Working in an Overcrowded Emergency Department in Taiwan.

PubMed

Chen, Li-Chin; Lin, Chun-Chih; Han, Chin-Yen; Hsieh, Chun-Lan; Wu, Chiung-Jung Jo; Liang, Hwey-Fang

2018-03-01

This study aims to gain in-depth understanding of nurses' perspectives of working in an overcrowded emergency. Symbolic interactionism and Charmaz's construction of grounded theory were used. Purposive sampling at the start of the study and a further theoretical sampling by snowball technique were used to recruit 40 registered nurses (RN) to participate in in-depth, semi-structured interviews between May and November, 2014. Data analysis included analytic techniques of initial, focused and theoretical coding. Study findings showed searching for work role is derived by the themes of Finding the role of positioning in Emergency Department (ED), Recognizing causes of ED overcrowding, and Confined working environment. Nurses' work experience which represents the RNs not gained control over their work, as care actions influenced by the problematic overcrowded circumstance of the ED environment. The findings fill a gap in knowledge about how RNs experience their work role in the context of an overcrowded Emergency Department in Taiwan. Arising from the study result include taking account of nurses' perspectives when planning staff/patient ratios, strategies to reduce waiting time and ensure that clients receive appropriate care. Copyright © 2018. Published by Elsevier B.V.

19 CFR 151.52 - Sampling procedures.

Code of Federal Regulations, 2012 CFR

2012-04-01

.... Representative commercial moisture and assay samples shall be taken under Customs supervision for testing by the Customs laboratory. The samples used for the moisture test shall be representative of the shipment at the... verified commercial moisture sample and prepared assay sample certified to be representative of the...

Cross-sectional study of height and weight in the population of Andalusia from age 3 to adulthood

PubMed Central

López-Siguero, Juan Pedro; García, Juan Manuel Fernández; Castillo, Juan de Dios Luna; Molina, Jose Antonio Moreno; Cosano, Carlos Ruiz; Ortiz, Antonio Jurado

2008-01-01

Background and objectives In Andalusia there were no studies including a representative sample of children and adolescent population assessing growth and weight increase. Our objectives were to develop reference standards for weight, height and BMI for the Andalusian pediatric population, from 3 to 18 years of age for both genders, and to identify the final adult height in Andalusia. Subjects and methods Two samples were collected. The first included individuals from 3 to 18 years of age (3592 girls and 3605 boys). They were stratified according type of study center, size of population of origin, age (32 categories of 0.5 years) and gender, using cluster sampling. Subjects from >18 to 23 years of age (947 women and 921 men) were sampled in 6 non-university educational centers and several university centers in Granada. Exclusion criteria included sons of non-Spanish mother or father, and individuals with chronic conditions and/or therapies affecting growth. Two trained fellows collected the data through February to December 2004, for the first sample, and through January to May 2005, for the second. Reference curves were adjusted using Cole's LMS method, and the quality of the adjustment was assessed using the tests proposed by Royston. In addition, a sensitivity analysis was applied to the final models obtained. Results Data for 9065 cases (4539 women and 4526 men) were obtained; 79.39% (n = 7197) in the up to 18 years of age group. In the first sampling only 0.07% (3 girls and 2 boys) refused to participate in the study. In addition, 327 students (4.5%) were absent when sampling was done. We present mean and standard deviation fort height, weight and BMI at 0.5 years intervals, from 3 to 23 years of age, for both genders. After adjustment with the different models, percentiles for height, weight (percentiles 3, 5, 10, 25, 50, 75, 90, 95, and 97) and BMI (percentiles 3, 5, 50, 85, 95, and 97) are presented for both genders. Conclusion This is the first study in Andalusia with a representative sample from the child-juvenile population to investigate weight, height and BMI in subjects from 3 to 23 years of age. The great variability observed in the values from sample of 18 to 23 years of age individuals, ensures the inclusion of extreme values, although random sampling was not used. There still is a lack of standard reference values for the Andalusian population younger done 3 years of age. PMID:18673524

Educating Homeless Children. Hearing before the Subcommittee on Early Childhood, Youth and Families of the Committee on Education and the Workforce. House of Representatives, One Hundred Sixth Congress, Second Session (Phoenix, Arizona, September 5, 2000).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. House Committee on Education and the Workforce.

This hearing before the Subcommittee on Early Childhood, Youth and Families of the Committee on Education and the Workforce, House of Representatives, which was held in Phoenix, Arizona, focused on ensuring equal educational opportunities for homeless children. After an opening statement by the Honorable Matt Solomon, Subcommittee on Early…

Safer@home—Simulation and training: the study protocol of a qualitative action research design

PubMed Central

Wiig, Siri; Guise, Veslemøy; Anderson, Janet; Storm, Marianne; Lunde Husebø, Anne Marie; Testad, Ingelin; Søyland, Elsa; Moltu, Kirsti L

2014-01-01

Introduction While it is predicted that telecare and other information and communication technology (ICT)-assisted services will have an increasingly important role in future healthcare services, their implementation in practice is complex. For implementation of telecare to be successful and ensure quality of care, sufficient training for staff (healthcare professionals) and service users (patients) is fundamental. Telecare training has been found to have positive effects on attitudes to, sustained use of, and outcomes associated with telecare. However, the potential contribution of training in the adoption, quality and safety of telecare services is an under-investigated research field. The overall aim of this study is to develop and evaluate simulation-based telecare training programmes to aid the use of videophone technology in elderly home care. Research-based training programmes will be designed for healthcare professionals, service users and next of kin, and the study will explore the impact of training on adoption, quality and safety of new telecare services. Methods and analysis The study has a qualitative action research design. The research will be undertaken in close collaboration with a multidisciplinary team consisting of researchers and managers and clinical representatives from healthcare services in two Norwegian municipalities, alongside experts in clinical education and simulation, as well as service user (patient) representatives. The qualitative methods used involve focus group interviews, semistructured interviews, observation and document analysis. To ensure trustworthiness in the data analysis, we will apply member checks and analyst triangulation; in addition to providing contextual and sample description to allow for evaluation of transferability of our results to other contexts and groups. Ethics and dissemination The study is approved by the Norwegian Social Science Data Services. The study is based on voluntary participation and informed written consent. Informants can withdraw at any point in time. The results will be disseminated at research conferences, peer review journals, one PhD thesis and through public presentations to people outside the scientific community. PMID:25079924

Local health department activities to ensure access to care.

PubMed

Luo, Huabin; Sotnikov, Sergey; Shah, Gulzar

2013-12-01

Local health departments (LHDs) can play an important role in linking people to personal health services and ensuring the provision of health care when it is otherwise unavailable. However, the extent to which LHDs are involved in ensuring access to health care in its jurisdictions is not well known. To provide nationally representative estimates of LHD involvement in specific activities to ensure access to healthcare services and to assess their association with macro-environment/community and LHD capacity and process characteristics. Data used were from the 2010 National Profile of Local Health Departments Study, Area Resource Files, and the Association of State and Territorial Health Officials' 2010 Profile of State Public Health Agencies Survey. Data were analyzed in 2012. Approximately 66.0% of LHDs conducted activities to ensure access to medical care, 45.9% to dental care, and 32.0% to behavioral health care. About 28% of LHDs had not conducted activities to ensure access to health care in their jurisdictions in 2010. LHDs with higher per capita expenditures and larger jurisdiction population sizes were more likely to provide access to care services (p <0.05). There is substantial variation in LHD engagement in activities to ensure access to care. Differences in LHD capacity and the needs of the communities in which they are located may account for this variation. Further research is needed to determine whether this variation is associated with adverse population health outcomes. © 2013 American Journal of Preventive Medicine Published by American Journal of Preventive Medicine All rights reserved.

Sealed magic angle spinning nuclear magnetic resonance probe and process for spectroscopy of hazardous samples

DOEpatents

Cho, Herman M.; Washton, Nancy M.; Mueller, Karl T.; Sears, Jr., Jesse A.; Townsend, Mark R.; Ewing, James R.

2016-06-14

A magic-angle-spinning (MAS) nuclear magnetic resonance (NMR) probe is described that includes double containment enclosures configured to seal and contain hazardous samples for analysis. The probe is of a modular design that ensures containment of hazardous samples during sample analysis while preserving spin speeds for superior NMR performance and convenience of operation.

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR FIELD COLLECTION OF INDOOR FLOOR SAMPLES AND POST FIELD SAMPLE HANDLING (UA-F-7.1)

EPA Science Inventory

The purpose of this SOP is to establish a uniform procedure for the collection of indoor floor dust samples in the field. This procedure was followed to ensure consistent data retrieval of dust samples during the Arizona NHEXAS project and the "Border" study. Keywords: field; va...

High Performance Computing and Communications Act of 1991. Hearing Before the Subcommittee on Science, Technology, and Space of the Committee on Commerce, Science, and Transportation. One Hundred Second Congress, First Session on S. 272 To Provide for a Coordinated Federal Research Program To Ensure Continued United States Leadership in High-Performance Computing.

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. Senate Committee on Commerce, Science, and Transportation.

This hearing before the Senate Subcommittee on Science, Technology, and Space focuses on S. 272, the High-Performance Computing and Communications Act of 1991, a bill that provides for a coordinated federal research and development program to ensure continued U.S. leadership in this area. Performance computing is defined as representing the…

7 CFR 3565.402 - Servicing responsibilities.

Code of Federal Regulations, 2010 CFR

2010-01-01

...) Asset management. The lender must ensure that the property securing the guaranteed loan remains in good physical and financial condition, in accordance with project management requirements contained in subpart H... its agents and representatives. (a) Funds management. The lender must have a funds management system...

Equations for Automotive-Transmission Performance

NASA Technical Reports Server (NTRS)

Chazanoff, S.; Aston, M. B.; Chapman, C. P.

1984-01-01

Curve-fitting procedure ensures high confidence levels. Threedimensional plot represents performance of small automatic transmission coasting in second gear. In equation for plot, PL power loss, S speed and T torque. Equations applicable to manual and automatic transmissions over wide range of speed, torque, and efficiency.

CHILDREN AS A SENSITIVE SUBPOPULATION FOR THE RISK ASSESSMENT PROCESS

EPA Science Inventory

Children as a sensitive subpopulation for the risk assessment process
Abstract
For cancer risk assessment purposes, it is necessary to consider how to incorporate sensitive subpopulations into the process to ensure that they are appropriately protected. Children represent o...

25 CFR 256.22 - How can I be sure that the work that is being done on my dwelling meets minimum construction...

Code of Federal Regulations, 2012 CFR

2012-04-01

... construction, a trained and qualified servicing housing office representative or building inspector will review the construction to ensure that it meets applicable minimum construction standards and building codes...

25 CFR 256.22 - How can I be sure that the work that is being done on my dwelling meets minimum construction...

Code of Federal Regulations, 2013 CFR

2013-04-01

... construction, a trained and qualified servicing housing office representative or building inspector will review the construction to ensure that it meets applicable minimum construction standards and building codes...

25 CFR 256.22 - How can I be sure that the work that is being done on my dwelling meets minimum construction...

Code of Federal Regulations, 2014 CFR

2014-04-01

... construction, a trained and qualified servicing housing office representative or building inspector will review the construction to ensure that it meets applicable minimum construction standards and building codes...

25 CFR 256.22 - How can I be sure that the work that is being done on my dwelling meets minimum construction...

Code of Federal Regulations, 2011 CFR

2011-04-01

... construction, a trained and qualified servicing housing office representative or building inspector will review the construction to ensure that it meets applicable minimum construction standards and building codes...

7 CFR 3565.402 - Servicing responsibilities.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Asset management. The lender must ensure that the property securing the guaranteed loan remains in good physical and financial condition, in accordance with project management requirements contained in subpart H... its agents and representatives. (a) Funds management. The lender must have a funds management system...

7 CFR 3565.402 - Servicing responsibilities.

Code of Federal Regulations, 2012 CFR

2012-01-01

...) Asset management. The lender must ensure that the property securing the guaranteed loan remains in good physical and financial condition, in accordance with project management requirements contained in subpart H... its agents and representatives. (a) Funds management. The lender must have a funds management system...

7 CFR 3565.402 - Servicing responsibilities.

Code of Federal Regulations, 2014 CFR

2014-01-01

...) Asset management. The lender must ensure that the property securing the guaranteed loan remains in good physical and financial condition, in accordance with project management requirements contained in subpart H... its agents and representatives. (a) Funds management. The lender must have a funds management system...

7 CFR 3565.402 - Servicing responsibilities.

Code of Federal Regulations, 2013 CFR

2013-01-01

...) Asset management. The lender must ensure that the property securing the guaranteed loan remains in good physical and financial condition, in accordance with project management requirements contained in subpart H... its agents and representatives. (a) Funds management. The lender must have a funds management system...

25 CFR 256.22 - How can I be sure that the work that is being done on my dwelling meets minimum construction...

Code of Federal Regulations, 2010 CFR

2010-04-01

... construction, a trained and qualified servicing housing office representative or building inspector will review the construction to ensure that it meets applicable minimum construction standards and building codes...

21 CFR 26.1 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-04-01

... (GMP's). [The United States has clarified its interpretation that under the MRA, paragraph (c)(1) of...) GMP's mean the requirements found in the legislations, regulations, and administrative provisions for... represented to possess. (2) GMP's are that part of quality assurance which ensures that products are...

The Complex Refractive Index of Volcanic Ash Aerosol Retrieved From Spectral Mass Extinction

NASA Astrophysics Data System (ADS)

Reed, Benjamin E.; Peters, Daniel M.; McPheat, Robert; Grainger, R. G.

2018-01-01

The complex refractive indices of eight volcanic ash samples, chosen to have a representative range of SiO2 contents, were retrieved from simultaneous measurements of their spectral mass extinction coefficient and size distribution. The mass extinction coefficients, at 0.33-19 μm, were measured using two optical systems: a Fourier transform spectrometer in the infrared and two diffraction grating spectrometers covering visible and ultraviolet wavelengths. The particle size distribution was measured using a scanning mobility particle sizer and an optical particle counter; values for the effective radius of ash particles measured in this study varied from 0.574 to 1.16 μm. Verification retrievals on high-purity silica aerosol demonstrated that the Rayleigh continuous distribution of ellipsoids (CDEs) scattering model significantly outperformed Mie theory in retrieving the complex refractive index, when compared to literature values. Assuming the silica particles provided a good analogue of volcanic ash, the CDE scattering model was applied to retrieve the complex refractive index of the eight ash samples. The Lorentz formulation of the complex refractive index was used within the retrievals as a convenient way to ensure consistency with the Kramers-Kronig relation. The short-wavelength limit of the electric susceptibility was constrained by using independently measured reference values of the complex refractive index of the ash samples at a visible wavelength. The retrieved values of the complex refractive indices of the ash samples showed considerable variation, highlighting the importance of using accurate refractive index data in ash cloud radiative transfer models.

SymPix: A Spherical Grid for Efficient Sampling of Rotationally Invariant Operators

NASA Astrophysics Data System (ADS)

Seljebotn, D. S.; Eriksen, H. K.

2016-02-01

We present SymPix, a special-purpose spherical grid optimized for efficiently sampling rotationally invariant linear operators. This grid is conceptually similar to the Gauss-Legendre (GL) grid, aligning sample points with iso-latitude rings located on Legendre polynomial zeros. Unlike the GL grid, however, the number of grid points per ring varies as a function of latitude, avoiding expensive oversampling near the poles and ensuring nearly equal sky area per grid point. The ratio between the number of grid points in two neighboring rings is required to be a low-order rational number (3, 2, 1, 4/3, 5/4, or 6/5) to maintain a high degree of symmetries. Our main motivation for this grid is to solve linear systems using multi-grid methods, and to construct efficient preconditioners through pixel-space sampling of the linear operator in question. As a benchmark and representative example, we compute a preconditioner for a linear system that involves the operator \\widehat{{\\boldsymbol{D}}}+{\\widehat{{\\boldsymbol{B}}}}T{{\\boldsymbol{N}}}-1\\widehat{{\\boldsymbol{B}}}, where \\widehat{{\\boldsymbol{B}}} and \\widehat{{\\boldsymbol{D}}} may be described as both local and rotationally invariant operators, and {\\boldsymbol{N}} is diagonal in the pixel domain. For a bandwidth limit of {{\\ell }}{max} = 3000, we find that our new SymPix implementation yields average speed-ups of 360 and 23 for {\\widehat{{\\boldsymbol{B}}}}T{{\\boldsymbol{N}}}-1\\widehat{{\\boldsymbol{B}}} and \\widehat{{\\boldsymbol{D}}}, respectively, compared with the previous state-of-the-art implementation.

U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR CUSTODY OF FIELD SAMPLES (UA-C-1.0)

EPA Science Inventory

The purpose of this SOP is to outline sample custody guidelines used by the Arizona Border Study. This procedure was followed to maintain sample integrity and ensure consistent data retrieval, transport, storage, shipment, and analysis during the Arizona NHEXAS project and the B...

The value of banked samples for oncology drug discovery and development.

PubMed

Shaw, Peter M; Patterson, Scott D

2011-01-01

To gain insights into human biology and pathobiology, ready access to banked human tissue samples that encompass a representative cross section of the population is required. For optimal use, the banked human tissue needs to be appropriately consented, collected, annotated, and stored. If any of these elements are missing, the studies using these samples are compromised. These elements are critical whether the research is for academic or pharmaceutical industry purposes. An additional temporal element that adds enormous value to such banked samples is treatment and outcome information from the people who donated the tissue. To achieve these aims, many different groups have to work effectively together, not least of which are the individuals who donate their tissue with appropriate consent. Such research is unlikely to benefit the donors but others who succumb to the same disease. The development of a large accessible human tissue bank resource (National Cancer Institute's Cancer HUman Biobank [caHUB]) that provides an ongoing supply of human tissue for all working toward the common goal of understanding human health and disease has a number of advantages. These include, but are not limited to, access to a broad cross section of healthy and diseased populations beyond what individual collections may achieve for understanding disease pathobiology, therapeutic target discovery, as well as a source of material for diagnostic assay validation. Models will need to be developed to enable fair access to caHUB under terms that enable appropriate intellectual property protection and ultimate data sharing to ensure that the biobank successfully distributes samples to a broad range of researchers.

Air-conditioning vs. presence of pathogenic fungi in hospital operating theatre environment.

PubMed

Gniadek, Agnieszka; Macura, Anna B

2011-01-01

Infections related to modern surgical procedures present a difficult problem for contemporary medicine. Infections acquired during surgery represent a risk factor related to therapeutical interventions. Eradication of microorganisms from hospital operating theatre environment may contribute to reduction of infections as the laminar flow air-conditioning considerably reduces the number of microorganisms in the hospital environment. The objective of the study was to evaluate the occurrence of fungi in air-conditioned operating theatre rooms. The study was carried out in one of the hospitals in Krak6w during December 2009. Indoor air samples and imprints from the walls were collected from five operating theatre rooms. A total of fifty indoor air samples were collected with a MAS-100 device, and twenty five imprints from the walls were collected using a Count Tact method. Fungal growth was observed in 48 air samples; the average numbers of fungi were within the range of 5-100 c.f.u. in one cubic metre of the air. Fungi were detected only in four samples of the wall imprints; the number of fungi was 0.01 c.f.u. per one square centimetre of the surface. The mould genus Aspergillus was most frequently isolated, and the species A. fumigatus and A. versicolor were the dominating ones. To ensure microbiological cleanness of hospital operating theatre, the air-conditioning system should be properly maintained. Domination of the Aspergillus fungi in indoor air as well as increase in the number of moulds in the samples taken in evenings (p < 0.05) may suggest that the room decontamination procedures were neglected.

Distribution of HPV genotypes in women with cervical cancer in Auckland, New Zealand; a review of 50 specimens between 2000-2006.

PubMed

Williamson, Deborah; Nagappan, Radhika; Sirikonda, Rao; Rahnama, Fahimeh; Thomas, Stephen; Lovell-Smith, Margaret; Croxson, Margaret

2011-02-01

In New Zealand, around two hundred women are diagnosed with cervical cancer annually, with approximately seventy deaths from cervical cancer per year. Our aim was to determine the distribution of oncogenic HPV genotypes in biopsy specimens from women with diagnosed cervical cancers in the Auckland region of New Zealand between 2000-2006. Confirmed cases of cervical carcinoma were identified from the local pathology register, and representative tissue samples were taken from these blocks. Sections were deparaffinised, and DNA was extracted according to standard protocols. Samples were subject to PCR amplification using L1 consensus primer sets MY09/11 and GP5/6. Further type-specific amplification was performed on positive samples, using an in-house primer sequence based on target sequences within the E6 gene. Remaining samples were typed by a Linear Array Assay, or by DNA sequencing. HPV DNA was detected in 100% of cases. In 49/50 samples, the HPV genotype was identified, with a total of 14 different HPV genotypes detectable. Together HPV-16 and 18 were found in 41/49 cases (83.6%) either singly or in combination. Our findings suggest that the distribution of HPV genotypes in New Zealand is similar to that of other geographic areas. Ongoing surveillance is warranted to ensure appropriate genotype selection for prophylactic HPV vaccinations. © 2010 The Authors. Australian and New Zealand Journal of Obstetrics and Gynaecology © 2010 The Royal Australian and New Zealand College of Obstetricians and Gynaecologists.

Airborne microbial composition in a high-throughput poultry slaughtering facility.

PubMed

Liang, Ruiping; Tian, Jijing; She, Ruiping; Meng, Hua; Xiao, Peng; Chang, Lingling

2013-03-01

A high-throughput chicken slaughtering facility in Beijing was systematically investigated for numbers of airborne microorganisms. Samples were assessed for counts of aerobic bacteria, Staphylococcus aureus, total coliforms, Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes, Bacillus cereus, and Salmonella. During a 4-month period (September to December 2011), samples were collected for 10 min three times daily (preproduction, production, and postproduction). Samples were collected for three consecutive days of each month with an FA-1 sampler from six sampling sites: receiving-hanging, soaking-scalding and defeathering, evisceration, precooling, subdividing, and packing. Humidity, temperature, wind velocity, and airborne particulates also were recorded at each sampling site and time. The highest counts of microorganisms were recorded in the initial stages of processing, i.e., the receiving-hanging and defeathering areas, with a definite decline toward the evisceration, prechilling, subdividing, and packing areas; the prechilling area had the lowest microbial counts of 2.4 × 10(3) CFU/m(3). Mean total coliforms counts ranged from 8.4 × 10(3) to 140 CFU/m(3). Maximum E. coli counts were 6.1 × 10(3) CFU/m(3) in the soaking-scalding and defeathering area. B. cereus, P. aeruginosa, and S. aureus represented only a small proportion of the microbial population (1,900 to 20 CFU/m(3)). L. monocytogenes and Salmonella were rarely detected in evisceration, precooling, subdividing, and packing areas. Our study identified the levels of bioaerosols that may affect chicken product quality. This finding could be useful for improved control of microbial contamination to ensure product quality.

What is Next? Linking all Samples of Planet Earth.

NASA Astrophysics Data System (ADS)

Wyborn, L. A.; Lehnert, K.; Klump, J. F.; Arko, R. A.; Cox, S. J. D.; Devaraju, A.; Elger, K.; Murphy, F.; Fleischer, D.

2016-12-01

The process of sampling, observing and analyzing physical samples is not unique to the geosciences. Physical sampling (taking specimens) is a fundamental strategy in many natural sciences, typically to support ex-situ observations in laboratories with the goal of characterizing real-world entities or populations. Observations and measurements are made on individual specimens and their derived samples in various ways, with results reported in research publications. Research on an individual sample is often published in numerous articles, based on multiple, potentially unrelated research programs conducted over many years. Even high-volume Earth observation datasets are proxies of real world phenomena and require calibration by measurements made on position located, well described physical samples. Unique, persistent web-compatible identifiers for physical objects and related sampling features are required to ensure their unambiguous citation and connection to related datasets through web identifiers. Identifier systems have been established within specific domains (e.g., bio, geo, hydro) or different sectors (e.g., museums, government agencies, universities), including the International Geo Sample Number (IGSN) in the geosciences, which has been used for rock, fossil, mineral, soil, regolith, fluid, plant and synthetic materials. IGSNs are issued through a governance system that ensures they are globally unique. Each IGSN directs to a digital representation of the physical object via the Handle.net global resolver system, the same system used for resolving DOI. To enable the unique identification of all samples on Planet Earth and of data derived from them, the next step is to ensure IGSNs can either be integrated with comparable identifier systems in other domains/sectors, or introduced into domains that do not have a viable system. A registry of persistent identifier systems for physical samples would allow users to choose which system best suits their needs. Such a registry may also facilitate unifying best practice in these multiple systems to enable consistent referencing of physical samples and of methods used to link digital data to its sources. IGSNs could be extended into other domains, but additional methodologies of sample collection, curation and processing may need to be considered.

Nevada National Security Site Integrated Groundwater Sampling Plan, Revision 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wilborn, Bill R.; Boehlecke, Robert F.

The purpose is to provide a comprehensive, integrated approach for collecting and analyzing groundwater samples to meet the needs and objectives of the DOE/EM Nevada Program’s UGTA Activity. Implementation of this Plan will provide high-quality data required by the UGTA Activity for ensuring public protection in an efficient and cost-effective manner. The Plan is designed to ensure compliance with the UGTA Quality Assurance Plan (QAP) (NNSA/NFO, 2015); Federal Facility Agreement and Consent Order (FFACO) (1996, as amended); and DOE Order 458.1, Radiation Protection of the Public and the Environment (DOE, 2013). The Plan’s scope comprises sample collection and analysis requirementsmore » relevant to assessing both the extent of groundwater contamination from underground nuclear testing and impact of testing on water quality in downgradient communities. This Plan identifies locations to be sampled by CAU and location type, sampling frequencies, sample collection methodologies, and the constituents to be analyzed. In addition, the Plan defines data collection criteria such as well purging, detection levels, and accuracy requirements/recommendations; identifies reporting and data management requirements; and provides a process to ensure coordination between NNSS groundwater sampling programs for sampling analytes of interest to UGTA. Information used in the Plan development—including the rationale for selection of wells, sampling frequency, and the analytical suite—is discussed under separate cover (N-I, 2014) and is not reproduced herein. This Plan does not address compliance for those wells involved in a permitted activity. Sampling and analysis requirements associated with these wells are described in their respective permits and are discussed in NNSS environmental reports (see Section 5.2). In addition, sampling for UGTA CAUs that are in the Closure Report (CR) stage are not included in this Plan. Sampling requirements for these CAUs are described in the CR. Frenchman Flat is currently the only UGTA CAU in the CR stage. Sampling requirements for this CAU are described in Underground Test Area (UGTA) Closure Report for Corrective Action Unit 98: Frenchman Flat Nevada National Security Site, Nevada (NNSA/NFO, 2016).« less

21 CFR 212.61 - What must I do to ensure the stability of my PET drug products through expiry?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 4 2010-04-01 2010-04-01 false What must I do to ensure the stability of my PET... the stability of my PET drug products through expiry? (a) Stability testing program. You must... PET drug products. The test methods must be reliable, meaningful, and specific. The samples tested for...

Heavy metal content of combustible municipal solid waste in Denmark.

PubMed

Riber, Christian; Fredriksen, Gry S; Christensen, Thomas H

2005-04-01

Data on the heavy metal composition of outlets from Danish incinerators was used to estimate the concentration of Zn, Cu, Pb, Cr, Ni, Cd, As and Hg in combustible waste (wet as received) at 14 Danish incinerators, representing about 80% of the waste incinerated in Denmark. Zn (1020 mg kg(-1)), Cu (620 mg kg(-1)) and Pb (370 mg kg(-1)) showed the highest concentration, whereas Hg (0.6 mg kg(-1)) showed the lowest concentration. The variation among the incinerators was in most cases within a factor of two to three, except for Cr that in two cases showed unexplained high concentrations. The fact that the data represent many incinerators and, in several cases, observations from a period of 4 to 5 years provides a good statistical basis for evaluating the content of heavy metals in combustible Danish waste. Such data may be used for identifying incinerators receiving waste with high concentrations of heavy metals suggesting the introduction of source control, or, if repeated in time, the data must also be used for monitoring the impacts of national regulation controlling heavy metals. It is recommended that future investigations consider the use of sample digestion methods that ensure complete digestion in order to use the data for determining the total heavy metal content of waste.

Mycotoxin Contamination in the EU Feed Supply Chain: A Focus on Cereal Byproducts.

PubMed

Pinotti, Luciano; Ottoboni, Matteo; Giromini, Carlotta; Dell'Orto, Vittorio; Cheli, Federica

2016-02-15

Mycotoxins represent a risk to the feed supply chain with an impact on economies and international trade. A high percentage of feed samples have been reported to be contaminated with more than one mycotoxin. In most cases, the concentrations were low enough to ensure compliance with the European Union (EU) guidance values or maximum admitted levels. However, mycotoxin co-contamination might still exert adverse effects on animals due to additive/synergistic interactions. Studies on the fate of mycotoxins during cereal processing, such as milling, production of ethanol fuels, and beer brewing, have shown that mycotoxins are concentrated into fractions that are commonly used as animal feed. Published data show a high variability in mycotoxin repartitioning, mainly due to the type of mycotoxins, the level and extent of fungal contamination, and a failure to understand the complexity of food processing technologies. Precise knowledge of mycotoxin repartitioning during technological processes is critical and may provide a sound technical basis for feed managers to conform to legislation requirements and reduce the risk of severe adverse market and trade repercussions. Regular, economical and straightforward feed testing is critical to reach a quick and accurate diagnosis of feed quality. The use of rapid methods represents a future challenge.

Mycotoxin Contamination in the EU Feed Supply Chain: A Focus on Cereal Byproducts

PubMed Central

Pinotti, Luciano; Ottoboni, Matteo; Giromini, Carlotta; Dell’Orto, Vittorio; Cheli, Federica

2016-01-01

Mycotoxins represent a risk to the feed supply chain with an impact on economies and international trade. A high percentage of feed samples have been reported to be contaminated with more than one mycotoxin. In most cases, the concentrations were low enough to ensure compliance with the European Union (EU) guidance values or maximum admitted levels. However, mycotoxin co-contamination might still exert adverse effects on animals due to additive/synergistic interactions. Studies on the fate of mycotoxins during cereal processing, such as milling, production of ethanol fuels, and beer brewing, have shown that mycotoxins are concentrated into fractions that are commonly used as animal feed. Published data show a high variability in mycotoxin repartitioning, mainly due to the type of mycotoxins, the level and extent of fungal contamination, and a failure to understand the complexity of food processing technologies. Precise knowledge of mycotoxin repartitioning during technological processes is critical and may provide a sound technical basis for feed managers to conform to legislation requirements and reduce the risk of severe adverse market and trade repercussions. Regular, economical and straightforward feed testing is critical to reach a quick and accurate diagnosis of feed quality. The use of rapid methods represents a future challenge. PMID:26891326

On the generation of climate model ensembles

NASA Astrophysics Data System (ADS)

Haughton, Ned; Abramowitz, Gab; Pitman, Andy; Phipps, Steven J.

2014-10-01

Climate model ensembles are used to estimate uncertainty in future projections, typically by interpreting the ensemble distribution for a particular variable probabilistically. There are, however, different ways to produce climate model ensembles that yield different results, and therefore different probabilities for a future change in a variable. Perhaps equally importantly, there are different approaches to interpreting the ensemble distribution that lead to different conclusions. Here we use a reduced-resolution climate system model to compare three common ways to generate ensembles: initial conditions perturbation, physical parameter perturbation, and structural changes. Despite these three approaches conceptually representing very different categories of uncertainty within a modelling system, when comparing simulations to observations of surface air temperature they can be very difficult to separate. Using the twentieth century CMIP5 ensemble for comparison, we show that initial conditions ensembles, in theory representing internal variability, significantly underestimate observed variance. Structural ensembles, perhaps less surprisingly, exhibit over-dispersion in simulated variance. We argue that future climate model ensembles may need to include parameter or structural perturbation members in addition to perturbed initial conditions members to ensure that they sample uncertainty due to internal variability more completely. We note that where ensembles are over- or under-dispersive, such as for the CMIP5 ensemble, estimates of uncertainty need to be treated with care.

Histopathological and histochemical changes in the liver of Bagrus bayad caused by environmental pollution.

PubMed

Gaber, Hanan S; Ibrahim, Seham A; El-Kasheif, Midhat A

2015-09-01

This study focused on two areas (Abu-Zaabal and El-Tal El-Kabir) of the Ismailia canal. The samples represented the effect of the factories' effluent discharge onto the canal. Physical characteristics (water temperature and pH) and chemical characteristics (total hardness, dissolved oxygen, biological oxygen demand and chemical oxygen demand) were measured to identify the water quality in the two areas of the Ismailia canal. These measurements showed slight variations between the two stations. Additionally, the histological and histochemical analyses in the liver of Bagrus bayad fish samples were done. Histological studies of liver showed more severe degeneration in the fish samples of El-Tal El-Kabir station. Histochemical studies on protein and glycogen contents in the liver of B. bayad showed a progressive decrease in their staining intensity to bromophenol blue and periodic acid Schiff at two stations. This study, therefore, concludes that Ismailia canal water (Abu-Zaabal and El-Tall El-Kabir) stations create metabolic crisis and impairment in fish liver. The depletion in the levels of protein and glycogen points leads to the exhaustion of cell energy that is of high demand for fish during stressful conditions. Therefore, it is imperative for the authorities concerned to ensure that treated effluent discharge comply with acceptable standards to save our environment from destruction. © The Author(s) 2013.

Xylem resistance to embolism: presenting a simple diagnostic test for the open vessel artefact.

PubMed

Torres-Ruiz, José M; Cochard, Hervé; Choat, Brendan; Jansen, Steven; López, Rosana; Tomášková, Ivana; Padilla-Díaz, Carmen M; Badel, Eric; Burlett, Regis; King, Andrew; Lenoir, Nicolas; Martin-StPaul, Nicolas K; Delzon, Sylvain

2017-07-01

Xylem vulnerability to embolism represents an essential trait for the evaluation of the impact of hydraulics in plant function and ecology. The standard centrifuge technique is widely used for the construction of vulnerability curves, although its accuracy when applied to species with long vessels remains under debate. We developed a simple diagnostic test to determine whether the open-vessel artefact influences centrifuge estimates of embolism resistance. Xylem samples from three species with differing vessel lengths were exposed to less negative xylem pressures via centrifugation than the minimum pressure the sample had previously experienced. Additional calibration was obtained from non-invasive measurement of embolism on intact olive plants by X-ray microtomography. Results showed artefactual decreases in hydraulic conductance (k) for samples with open vessels when exposed to a less negative xylem pressure than the minimum pressure they had previously experienced. X-Ray microtomography indicated that most of the embolism formation in olive occurs at xylem pressures below -4.0 MPa, reaching 50% loss of hydraulic conductivity at -5.3 MPa. The artefactual reductions in k induced by centrifugation underestimate embolism resistance data of species with long vessels. A simple test is suggested to avoid this open vessel artefact and to ensure the reliability of this technique in future studies. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.

Characterization and nultivariate analysis of physical properties of processing peaches

USDA-ARS?s Scientific Manuscript database

Characterization of physical properties of fruits represents the first vital step to ensure optimal performance of fruit processing operations and is also a prerequisite in the development of new processing equipment. In this study, physical properties of engineering significance to processing of th...

40 CFR 1042.250 - Recordkeeping and reporting.

Code of Federal Regulations, 2010 CFR

2010-07-01

... buildup, steps you took to ensure that it represents production engines, any components you built... CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE MARINE COMPRESSION-IGNITION ENGINES AND VESSELS Certifying Engine Families § 1042.250 Recordkeeping and reporting. (a) Send the Designated Compliance Officer...

DECISION-SUPPORT TOOLS FOR PREDICTING THE PERFORMANCE OF WATER DISTRIBUTION AND WASTEWATER COLLECTION SYSTEMS

EPA Science Inventory

Water and wastewater infrastructure systems represent a major capital investment; utilities must ensure they are getting the highest yield possible on their investment, both in terms of dollars and water quality. Accurate information related to equipment, pipe characteristics, l...

DECISION-SUPPORT TOOLS FOR PREDICTING THE PERFORMANCE OF WATER DISTRIBUTION AND WASTEWATER COLLECTION SYSTEMS

EPA Science Inventory

Water and wastewater infrastructure systems represent a major capital investment; utilities must ensure they are getting the highest yield possible on their investment, both in terms of dollars and water quality. Accurate information related to equipment, pipe characteristics, lo...

Connected vehicle pilot deployment program phase 2 : data privacy plan – New York City.

DOT National Transportation Integrated Search

2016-12-27

This document represents a data privacy plan for ensuring the data privacy and security of those participating in the New York City connected vehicle pilot. Personally Identifiable Information (PII) and Sensitive PII (SPII) will be collected from var...

Life science research objectives and representative experiments for the space station

NASA Technical Reports Server (NTRS)

Johnson, Catherine C. (Editor); Arno, Roger D. (Editor); Mains, Richard (Editor)

1989-01-01

A workshop was convened to develop hypothetical experiments to be used as a baseline for space station designer and equipment specifiers to ensure responsiveness to the users, the life science community. Sixty-five intra- and extramural scientists were asked to describe scientific rationales, science objectives, and give brief representative experiment descriptions compatible with expected space station accommodations, capabilities, and performance envelopes. Experiment descriptions include hypothesis, subject types, approach, equipment requirements, and space station support requirements. The 171 experiments are divided into 14 disciplines.

U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR FIELD COLLECTION AND POST-FIELD SAMPLE HANDLING OF INDOOR FLOOR DUST SAMPLES (UA-F-7.1)

EPA Science Inventory

The purpose of this SOP is to establish a uniform procedure for the collection of indoor floor dust samples in the field. This procedure was followed to ensure consistent data retrieval of dust samples during the Arizona NHEXAS project and the Border study. Keywords: field; vacu...

Quota sampling in internet research: practical issues.

PubMed

Im, Eun-Ok; Chee, Wonshik

2011-07-01

Quota sampling has been suggested as a potentially good method for Internet-based research and has been used by several researchers working with Internet samples. However, very little is known about the issues or concerns in using a quota sampling method in Internet research. The purpose of this article was to present the practical issues using quota sampling in an Internet-based study. During the Internet study, the research team recorded all recruitment issues that arose and made written notes indicating the possible reasons for the problems. In addition, biweekly team discussions were conducted for which written records were kept. Overall, quota sampling was effective in ensuring that an adequate number of midlife women were recruited from the targeted ethnic groups. However, during the study process, we encountered the following practical issues using quota sampling: (1) difficulty reaching out to women in lower socioeconomic classes, (2) difficulty ensuring authenticity of participants' identities, (3) participants giving inconsistent answers for the screening questions versus the Internet survey questions, (4) potential problems with a question on socioeconomic status, (5) resentment toward the research project and/or researchers because of rejection, and (6) a longer time and more expense than anticipated.

Ensuring quality: a key consideration in scaling-up HIV-related point-of-care testing programs

PubMed Central

Fonjungo, Peter N.; Osmanov, Saladin; Kuritsky, Joel; Ndihokubwayo, Jean Bosco; Bachanas, Pam; Peeling, Rosanna W.; Timperi, Ralph; Fine, Glenn; Stevens, Wendy; Habiyambere, Vincent; Nkengasong, John N.

2016-01-01

Objective: The objective of the WHO/US President's Emergency Plan for AIDS Relief consultation was to discuss innovative strategies, offer guidance, and develop a comprehensive policy framework for implementing quality-assured HIV-related point-of-care testing (POCT). Methods: The consultation was attended by representatives from international agencies (WHO, UNICEF, UNITAID, Clinton Health Access Initiative), United States Agency for International Development, Centers for Disease Control and Prevention/President's Emergency Plan for AIDS Relief Cooperative Agreement Partners, and experts from more than 25 countries, including policy makers, clinicians, laboratory experts, and program implementers. Main outcomes: There was strong consensus among all participants that ensuring access to quality of POCT represents one of the key challenges for the success of HIV prevention, treatment, and care programs. The following four strategies were recommended: implement a newly proposed concept of a sustainable quality assurance cycle that includes careful planning; definition of goals and targets; timely implementation; continuous monitoring; improvements and adjustments, where necessary; and a detailed evaluation; the importance of supporting a cadre of workers [e.g. volunteer quality corps (Q-Corps)] with the role to ensure that the quality assurance cycle is followed and sustained; implementation of the new strategy should be seen as a step-wise process, supported by development of appropriate policies and tools; and joint partnership under the leadership of the ministries of health to ensure sustainability of implementing novel approaches. Conclusion: The outcomes of this consultation have been well received by program implementers in the field. The recommendations also laid the groundwork for developing key policy and quality documents for the implementation of HIV-related POCT. PMID:26807969

WTP Waste Feed Qualification: Glass Fabrication Unit Operation Testing Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stone, M. E.; Newell, J. D.; Johnson, F. C.

The waste feed qualification program is being developed to protect the Hanford Tank Waste Treatment and Immobilization Plant (WTP) design, safety basis, and technical basis by assuring waste acceptance requirements are met for each staged waste feed campaign prior to transfer from the Tank Operations Contractor to the feed receipt vessels inside the Pretreatment Facility. The Waste Feed Qualification Program Plan describes the three components of waste feed qualification: 1. Demonstrate compliance with the waste acceptance criteria 2. Determine waste processability 3. Test unit operations at laboratory scale. The glass fabrication unit operation is the final step in the processmore » demonstration portion of the waste feed qualification process. This unit operation generally consists of combining each of the waste feed streams (high-level waste (HLW) and low-activity waste (LAW)) with Glass Forming Chemicals (GFCs), fabricating glass coupons, performing chemical composition analysis before and after glass fabrication, measuring hydrogen generation rate either before or after glass former addition, measuring rheological properties before and after glass former addition, and visual observation of the resulting glass coupons. Critical aspects of this unit operation are mixing and sampling of the waste and melter feeds to ensure representative samples are obtained as well as ensuring the fabrication process for the glass coupon is adequate. Testing was performed using a range of simulants (LAW and HLW simulants), and these simulants were mixed with high and low bounding amounts of GFCs to evaluate the mixing, sampling, and glass preparation steps in shielded cells using laboratory techniques. The tests were performed with off-the-shelf equipment at the Savannah River National Laboratory (SRNL) that is similar to equipment used in the SRNL work during qualification of waste feed for the Defense Waste Processing Facility (DWPF) and other waste treatment facilities at the Savannah River Site. It is not expected that the exact equipment used during this testing will be used during the waste feed qualification testing for WTP, but functionally similar equipment will be used such that the techniques demonstrated would be applicable. For example, the mixing apparatus could use any suitable mixer capable of being remoted and achieving similar mixing speeds to those tested.« less

Multi-saline sample distillation apparatus for hydrogen isotope analyses : design and accuracy

USGS Publications Warehouse

Hassan, Afifa Afifi

1981-01-01

A distillation apparatus for saline water samples was designed and tested. Six samples may be distilled simultaneously. The temperature was maintained at 400 C to ensure complete dehydration of the precipitating salts. Consequently, the error in the measured ratio of stable hydrogen isotopes resulting from incomplete dehydration of hydrated salts during distillation was eliminated. (USGS)

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR FIELD COLLECTION OF YARD COMPOSITE SOIL SAMPLES (UA-F-5.1)

EPA Science Inventory

The purpose of this SOP is to establish a uniform procedure for the collection of yard composite soil samples in the field. This procedure was followed to ensure consistent and reliable collection of outdoor soil samples during the Arizona NHEXAS project and the "Border" study. ...

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR FIELD COLLECTION OF RESIDENTIAL FOUNDATION SOIL SAMPLES (UA-F-6.1)

EPA Science Inventory

The purpose of this SOP is to establish a uniform procedure for the collection of residential foundation soil samples in the field. This procedure was followed to ensure consistent and reliable collection of outdoor soil samples during the Arizona NHEXAS project and the "Border"...

Variation in aluminum, iron, and particle concentrations in oxic ground-water samples collected by use of tangential-flow ultrafiltration with low-flow sampling

USGS Publications Warehouse

Szabo, Z.; Oden, J.H.; Gibs, J.; Rice, D.E.; Ding, Y.; ,

2001-01-01

Particulates that move with ground water and those that are artificially mobilized during well purging could be incorporated into water samples during collection and could cause trace-element concentrations to vary in unfiltered samples, and possibly in filtered samples (typically 0.45-um (micron) pore size) as well, depending on the particle-size fractions present. Therefore, measured concentrations may not be representative of those in the aquifer. Ground water may contain particles of various sizes and shapes that are broadly classified as colloids, which do not settle from water, and particulates, which do. In order to investigate variations in trace-element concentrations in ground-water samples as a function of particle concentrations and particle-size fractions, the U.S. Geological Survey, in cooperation with the U.S. Air Force, collected samples from five wells completed in the unconfined, oxic Kirkwood-Cohansey aquifer system of the New Jersey Coastal Plain. Samples were collected by purging with a portable pump at low flow (0.2-0.5 liters per minute and minimal drawdown, ideally less than 0.5 foot). Unfiltered samples were collected in the following sequence: (1) within the first few minutes of pumping, (2) after initial turbidity declined and about one to two casing volumes of water had been purged, and (3) after turbidity values had stabilized at less than 1 to 5 Nephelometric Turbidity Units. Filtered samples were split concurrently through (1) a 0.45-um pore size capsule filter, (2) a 0.45-um pore size capsule filter and a 0.0029-um pore size tangential-flow filter in sequence, and (3), in selected cases, a 0.45-um and a 0.05-um pore size capsule filter in sequence. Filtered samples were collected concurrently with the unfiltered sample that was collected when turbidity values stabilized. Quality-assurance samples consisted of sequential duplicates (about 25 percent) and equipment blanks. Concentrations of particles were determined by light scattering. Variations in concentrations aluminum and iron (1 -74 and 1-199 ug/L (micrograms per liter), respectively), common indicators of the presence of particulate-borne trace elements, were greatest in sample sets from individual wells with the greatest variations in turbidity and particle concentration. Differences in trace-element concentrations in sequentially collected unfiltered samples with variable turbidity were 5 to 10 times as great as those in concurrently collected samples that were passed through various filters. These results indicate that turbidity must be both reduced and stabilized even when low-flow sample-collection techniques are used in order to obtain water samples that do not contain considerable particulate artifacts. Currently (2001) available techniques need to be refined to ensure that the measured trace-element concentrations are representative of those that are mobile in the aquifer water.

Outsourcing Technology in Government: Owned, Controlled, or Regulated Institutions.

ERIC Educational Resources Information Center

Gordon, Mark L.; Walsh, Timothy P.

1997-01-01

Examines the growing trend toward and objectives of government outsourcing of information technology (IT) both in the United States and around the world. Describes representative outsourcing arrangements; highlights steps governments should take to ensure that objectives are met; and discusses factors influencing management of public sector…

32 CFR Appendix B to Part 247 - CE Publications

Code of Federal Regulations, 2012 CFR

2012-07-01

... liaison functions on publisher premises including monitoring and coordinating layout and design and other... coordinate layout and ensure that the preparation of editorial material is performed in such a way as to... commander's representative shall have the authority to specify newspaper advertising layout when required to...

10 CFR 429.15 - Room air conditioners.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 3 2012-01-01 2012-01-01 false Room air conditioners. 429.15 Section 429.15 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION CERTIFICATION, COMPLIANCE, AND ENFORCEMENT FOR CONSUMER PRODUCTS AND... selected and tested to ensure that— (i) Any represented value of estimated annual operating cost, energy...

77 FR 58291 - National Hispanic Heritage Month, 2012

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-19

..., 2012 Proclamation 8865--National Farm Safety and Health Week, 2012 Proclamation 8866--National Hispanic... promoting job creation and ensuring Hispanics are represented in the Federal workforce to reshaping our education system to meet the demands of the 21st century, my Administration has built ladders of opportunity...

Empowering Youth to Take Charge of School Wellness

ERIC Educational Resources Information Center

Hughes, Luanne J.; Savoca, LeeAnne; Grenci, Alexandra

2015-01-01

Youth Advisory Councils (YACs) ensure that students are represented in school wellness discussions. YACs empower students to present ideas, insights, and input on nutrition and physical activity; work alongside peers to assess wellness needs; and develop recommendations for enhancing/expanding the school wellness environment. YACs provide a…

Predicting microbial water quality with models: over-arching questions for managing risk in agricultural catchments

USDA-ARS?s Scientific Manuscript database

Determining the microbial quality of recreational, irrigation and shellfish-harvesting waters is important to ensure compliance with health-related standards and associated legislation. Animal faeces represent a significant human health risk, and concentrations of fecal indicator organisms (FIOs) pr...

43 CFR 24.5 - International agreements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... boundaries. The authority to enter into such agreements is reserved to the President by and with the advice... that give strong consideration to established State programs designed to ensure the conservation of... advice of affected State agencies and to recommend to the U.S. Department of State that representatives...

43 CFR 24.5 - International agreements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... boundaries. The authority to enter into such agreements is reserved to the President by and with the advice... that give strong consideration to established State programs designed to ensure the conservation of... advice of affected State agencies and to recommend to the U.S. Department of State that representatives...

Gulf Coast Community College's Memory Project

ERIC Educational Resources Information Center

Burrell, Matthew D.

2005-01-01

Gulf Coast Community College in Panama City, Florida, is celebrating a fifty-year anniversary in 2007. Maintained by the library, the school's archives represent the historical contributions on a local and national level. Gulf Coast Community College library is ensuring the school's historical significance through the digitization of its…

Claiming Voice on the Future of Higher Education

ERIC Educational Resources Information Center

Roper, Larry D.

2016-01-01

Higher education reform is a prominent topic among state and federal governments. However, the discussions regarding higher education are narrowly focused and not always inclusive of the voices of postsecondary leaders. Higher education officials must find approaches to ensure their voices are appropriately represented in these crucial…

Rhetorical Structure of Biochemistry Research Articles

ERIC Educational Resources Information Center

Kanoksilapatham, Budsaba

2005-01-01

This paper reports on the results of a move analysis [Swales, J. (1990). "Genre analysis." Cambridge: Cambridge University Press] of 60 biochemistry research articles. First, a corpus was systematically compiled to ensure that it represents core journals in the focused discipline. Then, coding reliability analysis was conducted to…

38 CFR 18.444 - Academic adjustments.

Code of Federal Regulations, 2010 CFR

2010-07-01

... speaking skills that will best ensure that the results of the evaluation represent the students' achievement in the course, rather than reflect the students' impaired sensory, manual, or speaking skills (except where such skills are the factors that the test purports to measure). (d) Auxiliary aids. (1) A...

22 CFR 142.44 - Academic adjustments.

Code of Federal Regulations, 2010 CFR

2010-04-01

..., manual, speaking or other skills as will best ensure that the results of the evaluation represent the..., speaking or other skills (except where such skills are the factors that the test purports to measure). (d... impaired sensory, manual, speaking or other skills. (2) Auxiliary aids may include taped texts...

22 CFR 217.44 - Academic adjustments.

Code of Federal Regulations, 2010 CFR

2010-04-01

..., manual, or speaking skills as will best ensure that the results of the evaluation represents the student... speaking skills (except where such skills are the factors that the test purports to measure). (d) Auxiliary... sensory, manual, or speaking skills. (2) Auxiliary aids may include taped texts, interpreters or other...

Standards-Based Grading and Reporting Will Improve Education

ERIC Educational Resources Information Center

Muñoz, Marco A.; Guskey, Thomas R.

2015-01-01

Grading and reporting are foundational elements in nearly every educational system. Grading represents teachers' evaluations, formative or summative, of students' performance. Reporting is how the results of those evaluations are communicated to students, parents, or others. Because of their fundamental nature, educators must ensure that grading…

[Indication: scientific and ethical basis of medical practice].

PubMed

Neitzke, G

2014-02-01

The medical indication provides a rational foundation for treatment decisions. An indication can be defined as the reasonable professional judgement that a medical procedure is suitable and useful to reach a specific therapeutic goal with a certain probability. An indication includes empirical, causal and purposive considerations and justifications, and a scrutiny of the individual case. This ensures that medical aspects of the patient, therapeutic goals and evidence-based knowledge are integrated and represented in the indication. An indication justifies a treatment proposal to the patient, which may only be carried out after a procedure of informed consent. Indications can be considered as the ethical basis of treatment decisions and as the heart of professionalism: indications should be used as a professional tool to protect against irrational therapeutic expectations, while ensuring the patient's right for reasonable treatment. In health care that is increasingly shaped by economic constraints, conscientious indications ensure the professional and ethical basis of medical treatment decisions.

Increasing the speed of tumour diagnosis during surgery with selective scanning Raman microscopy

NASA Astrophysics Data System (ADS)

Kong, Kenny; Rowlands, Christopher J.; Varma, Sandeep; Perkins, William; Leach, Iain H.; Koloydenko, Alexey A.; Pitiot, Alain; Williams, Hywel C.; Notingher, Ioan

2014-09-01

One of the main challenges in cancer surgery is ensuring that all tumour cells are removed during surgery, while sparing as much healthy tissue as possible. Histopathology, the gold-standard technique for cancer diagnosis, is often impractical for intra-operative use because of the time-consuming tissue preparation procedures (sectioning and staining). Raman micro-spectroscopy is a powerful technique that can discriminate between tumours and healthy tissues with high accuracy, based entirely on intrinsic chemical differences. However, raster-scanning Raman micro-spectroscopy is a slow imaging technique that typically requires data acquisition times as long as several days for typical tissue samples obtained during surgery (1 × 1 cm2) - in particular when high signal-to-noise ratio spectra are required to ensure accurate diagnosis. In this paper we present two techniques based on selective sampling Raman micro-spectroscopy that can overcome these limitations. In selective sampling, information regarding the spatial features of the tissue, either measured by an alternative optical technique or estimated in real-time from the Raman spectra, can be used to drastically reduce the number of Raman spectra required for diagnosis. These sampling strategies allowed diagnosis of basal cell carcinoma in skin tissue samples excised during Mohs micrographic surgery faster than frozen section histopathology, and two orders of magnitude faster than previous techniques based on raster-scanning Raman microscopy. Further development of these techniques may help during cancer surgery by providing a fast and objective way for surgeons to ensure the complete removal of tumour cells while sparing as much healthy tissue as possible.

[South African biltong--another close look].

PubMed

Osterhoff, D R; Leistner, L

1984-12-01

Twenty beef biltong samples from various commercial sources were investigated chemically and microbiologically. The biltong contained 5-10% NaCl, little sugar and nitrite but 10-860 ppm nitrate. The addition of nitrate apparently did not ensure stability, since spoiled biltong often contained much residual nitrate. Biltong was stable at the barrier combination of aw less than or equal to 0.77 and pH less than or equal to 5.5 because such samples did not spontaneously become mouldy. A high degree of contamination with moulds and yeasts was found indicating a real health hazard to which consumers might be exposed. Better control measures must be imposed to ensure a better product to the consumer.

Improving the Quality of Web Surveys: The Checklist for Reporting Results of Internet E-Surveys (CHERRIES)

PubMed Central

2004-01-01

Analogous to checklists of recommendations such as the CONSORT statement (for randomized trials), or the QUORUM statement (for systematic reviews), which are designed to ensure the quality of reports in the medical literature, a checklist of recommendations for authors is being presented by the Journal of Medical Internet Research (JMIR) in an effort to ensure complete descriptions of Web-based surveys. Papers on Web-based surveys reported according to the CHERRIES statement will give readers a better understanding of the sample (self-)selection and its possible differences from a “representative” sample. It is hoped that author adherence to the checklist will increase the usefulness of such reports. PMID:15471760

On the ecological relevance of landscape mapping and its application in the spatial planning of very large marine protected areas.

PubMed

Hogg, Oliver T; Huvenne, Veerle A I; Griffiths, Huw J; Linse, Katrin

2018-06-01

In recent years very large marine protected areas (VLMPAs) have become the dominant form of spatial protection in the marine environment. Whilst seen as a holistic and geopolitically achievable approach to conservation, there is currently a mismatch between the size of VLMPAs, and the data available to underpin their establishment and inform on their management. Habitat mapping has increasingly been adopted as a means of addressing paucity in biological data, through use of environmental proxies to estimate species and community distribution. Small-scale studies have demonstrated environmental-biological links in marine systems. Such links, however, are rarely demonstrated across larger spatial scales in the benthic environment. As such, the utility of habitat mapping as an effective approach to the ecosystem-based management of VLMPAs remains, thus far, largely undetermined. The aim of this study was to assess the ecological relevance of broadscale landscape mapping. Specifically we test the relationship between broad-scale marine landscapes and the structure of their benthic faunal communities. We focussed our work at the sub-Antarctic island of South Georgia, site of one of the largest MPAs in the world. We demonstrate a statistically significant relationship between environmentally derived landscape mapping clusters, and the composition of presence-only species data from the region. To demonstrate this relationship required specific re-sampling of historical species occurrence data to balance biological rarity, biological cosmopolitism, range-restricted sampling and fine-scale heterogeneity between sampling stations. The relationship reveals a distinct biological signature in the faunal composition of individual landscapes, attributing ecological relevance to South Georgia's environmentally derived marine landscape map. We argue therefore, that landscape mapping represents an effective framework for ensuring representative protection of habitats in management plans. Such scientific underpinning of marine spatial planning is critical in balancing the needs of multiple stakeholders whilst maximising conservation payoff. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Automated novelty detection in the WISE survey with one-class support vector machines

NASA Astrophysics Data System (ADS)

Solarz, A.; Bilicki, M.; Gromadzki, M.; Pollo, A.; Durkalec, A.; Wypych, M.

2017-10-01

Wide-angle photometric surveys of previously uncharted sky areas or wavelength regimes will always bring in unexpected sources - novelties or even anomalies - whose existence and properties cannot be easily predicted from earlier observations. Such objects can be efficiently located with novelty detection algorithms. Here we present an application of such a method, called one-class support vector machines (OCSVM), to search for anomalous patterns among sources preselected from the mid-infrared AllWISE catalogue covering the whole sky. To create a model of expected data we train the algorithm on a set of objects with spectroscopic identifications from the SDSS DR13 database, present also in AllWISE. The OCSVM method detects as anomalous those sources whose patterns - WISE photometric measurements in this case - are inconsistent with the model. Among the detected anomalies we find artefacts, such as objects with spurious photometry due to blending, but more importantly also real sources of genuine astrophysical interest. Among the latter, OCSVM has identified a sample of heavily reddened AGN/quasar candidates distributed uniformly over the sky and in a large part absent from other WISE-based AGN catalogues. It also allowed us to find a specific group of sources of mixed types, mostly stars and compact galaxies. By combining the semi-supervised OCSVM algorithm with standard classification methods it will be possible to improve the latter by accounting for sources which are not present in the training sample, but are otherwise well-represented in the target set. Anomaly detection adds flexibility to automated source separation procedures and helps verify the reliability and representativeness of the training samples. It should be thus considered as an essential step in supervised classification schemes to ensure completeness and purity of produced catalogues. The catalogues of outlier data are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/606/A39

[Research on developping the spectral dataset for Dunhuang typical colors based on color constancy].

PubMed

Liu, Qiang; Wan, Xiao-Xia; Liu, Zhen; Li, Chan; Liang, Jin-Xing

2013-11-01

The present paper aims at developping a method to reasonably set up the typical spectral color dataset for different kinds of Chinese cultural heritage in color rendering process. The world famous wall paintings dating from more than 1700 years ago in Dunhuang Mogao Grottoes was taken as typical case in this research. In order to maintain the color constancy during the color rendering workflow of Dunhuang culture relics, a chromatic adaptation based method for developping the spectral dataset of typical colors for those wall paintings was proposed from the view point of human vision perception ability. Under the help and guidance of researchers in the art-research institution and protection-research institution of Dunhuang Academy and according to the existing research achievement of Dunhuang Research in the past years, 48 typical known Dunhuang pigments were chosen and 240 representative color samples were made with reflective spectral ranging from 360 to 750 nm was acquired by a spectrometer. In order to find the typical colors of the above mentioned color samples, the original dataset was devided into several subgroups by clustering analysis. The grouping number, together with the most typical samples for each subgroup which made up the firstly built typical color dataset, was determined by wilcoxon signed rank test according to the color inconstancy index comprehensively calculated under 6 typical illuminating conditions. Considering the completeness of gamut of Dunhuang wall paintings, 8 complementary colors was determined and finally the typical spectral color dataset was built up which contains 100 representative spectral colors. The analytical calculating results show that the median color inconstancy index of the built dataset in 99% confidence level by wilcoxon signed rank test was 3.28 and the 100 colors are distributing in the whole gamut uniformly, which ensures that this dataset can provide reasonable reference for choosing the color with highest color constancy during the color rendering process of Dunhuang cultural heritage.

40 CFR Appendix I to Part 261 - Representative Sampling Methods

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Representative Sampling Methods I Appendix I to Part 261 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES...—Representative Sampling Methods The methods and equipment used for sampling waste materials will vary with the...

Pulmonary Effects of Eight-Hour MK 16 MOD 1 Dives

DTIC Science & Technology

2007-10-01

carboxyhemoglobin and hemoglobin concentrations,5 and the samples were chosen to ensure that the analyzer signal was stable when measurements were recorded.6...Lexington, MA) determined the pretest carboxyhemoglobin and hemoglobin concentrations from a venous blood sample. An autorefractor (Humphrey model 599

Four-Hour Dives with Exercise While Breathing Oxygen Partial Pressure of 1.3 ATM

DTIC Science & Technology

2006-09-01

carboxyhemoglobin and hemoglobin concentrations, 8 and the samples were chosen to ensure that the analyzer signal was stable when measurements were...Instrumentation Laboratory; Lexington, MA) determined the pretest carboxyhemoglobin and hemoglobin concentrations from a venous blood sample. An autorefractor

Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy.

PubMed

Hermannsdörfer, Justus; de Jonge, Niels

2017-02-05

Samples fully embedded in liquid can be studied at a nanoscale spatial resolution with Scanning Transmission Electron Microscopy (STEM) using a microfluidic chamber assembled in the specimen holder for Transmission Electron Microscopy (TEM) and STEM. The microfluidic system consists of two silicon microchips supporting thin Silicon Nitride (SiN) membrane windows. This article describes the basic steps of sample loading and data acquisition. Most important of all is to ensure that the liquid compartment is correctly assembled, thus providing a thin liquid layer and a vacuum seal. This protocol also includes a number of tests necessary to perform during sample loading in order to ensure correct assembly. Once the sample is loaded in the electron microscope, the liquid thickness needs to be measured. Incorrect assembly may result in a too-thick liquid, while a too-thin liquid may indicate the absence of liquid, such as when a bubble is formed. Finally, the protocol explains how images are taken and how dynamic processes can be studied. A sample containing AuNPs is imaged both in pure water and in saline.

Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy

PubMed Central

Hermannsdörfer, Justus; de Jonge, Niels

2017-01-01

Samples fully embedded in liquid can be studied at a nanoscale spatial resolution with Scanning Transmission Electron Microscopy (STEM) using a microfluidic chamber assembled in the specimen holder for Transmission Electron Microscopy (TEM) and STEM. The microfluidic system consists of two silicon microchips supporting thin Silicon Nitride (SiN) membrane windows. This article describes the basic steps of sample loading and data acquisition. Most important of all is to ensure that the liquid compartment is correctly assembled, thus providing a thin liquid layer and a vacuum seal. This protocol also includes a number of tests necessary to perform during sample loading in order to ensure correct assembly. Once the sample is loaded in the electron microscope, the liquid thickness needs to be measured. Incorrect assembly may result in a too-thick liquid, while a too-thin liquid may indicate the absence of liquid, such as when a bubble is formed. Finally, the protocol explains how images are taken and how dynamic processes can be studied. A sample containing AuNPs is imaged both in pure water and in saline. PMID:28190028

COMPARISON OF ECOLOGICAL COMMUNITIES: THE PROBLEM OF SAMPLE REPRESENTATIVENESS

EPA Science Inventory

Obtaining an adequate, representative sample of ecological communities to make taxon richness (TR) or compositional comparisons among sites is a continuing challenge. Sample representativeness literally means the similarity in species composition and relative abundance between a ...

Long-Term Impacts Induced by Disposal of Contaminated River Sediments in Elliott Bay, Seattle, Washington

DTIC Science & Technology

1984-09-01

and these accompanied the sample residue through sieving to avoid sample mix- up . B. Field data sheets required logger’s initials on each page to A...ensure data completeness. C. Metal trays were placed to catch residue spillage during residue transfer from sieves to sample bottles. D. Sample bottles...methodologies were comparable for all sample e types and consisted of four sequential components: extraction, clean- up , gas chromatographic (GC) analysis, and

Does the Janani Suraksha Yojana cash transfer programme to promote facility births in India ensure skilled birth attendance? A qualitative study of intrapartum care in Madhya Pradesh.

PubMed

Chaturvedi, Sarika; De Costa, Ayesha; Raven, Joanna

2015-01-01

Access to facility delivery in India has significantly increased with the Janani Suraksha Yojana (JSY) cash transfer programme to promote facility births. However, a decline in maternal mortality has only followed secular trends as seen from the beginning of the decade well before the programme began. We, therefore, examined the quality of intrapartum care provided in facilities under the JSY programme to study whether it ensures skilled attendance at birth. 1) Non-participant observations (n=18) of intrapartum care during vaginal deliveries at a representative sample of 11 facilities in Madhya Pradesh to document what happens during intrapartum care. 2) Interviews (n=10) with providers to explore reasons for this care. Thematic framework analysis was used. Three themes emerged from the data: 1) delivery environment is chaotic: delivery rooms were not conducive to safe, women-friendly care provision, and coordination between providers was poor. 2) Staff do not provide skilled care routinely: this emerged from observations that monitoring was limited to assessment of cervical dilatation, lack of readiness to provide key elements of care, and the execution of harmful/unnecessary practices coupled with poor techniques. 3) Dominant staff, passive recipients: staff sometimes threatened, abused, or ignored women during delivery; women were passive and accepted dominance and disrespect. Attendants served as 'go-betweens' patients and providers. The interviews with providers revealed their awareness of the compromised quality of care, but they were constrained by structural problems. Positive practices were also observed, including companionship during childbirth and women mobilising in the early stages of labour. Our observational study did not suggest an adequate level of skilled birth attendance (SBA). The findings reveal insufficiencies in the health system and organisational structures to provide an 'enabling environment' for SBA. We highlight the need to ensure quality obstetric care prior to increasing coverage of facility births if cash transfer programmes like the JSY are to improve health outcomes.

The Importance of Simulation in Nursing Education

ERIC Educational Resources Information Center

Eyikara, Evrim; Baykara, Zehra Gocmen

2017-01-01

Nursing education involves a practice-oriented curriculum in which emphasis is placed on both theoretical knowledge and psychomotor skills. In skill-based education, where learning through practice occupies a central role, it is important to ensure the integration of theoretical knowledge into practice. In this context, simulations represent an…

10 CFR 429.54 - Metal halide lamp ballasts and fixtures.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 3 2014-01-01 2014-01-01 false Metal halide lamp ballasts and fixtures. 429.54 Section 429.54 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION CERTIFICATION, COMPLIANCE, AND ENFORCEMENT FOR... and tested to ensure that: (i) Any represented value of estimated energy efficiency calculated as the...

10 CFR 429.54 - Metal halide lamp ballasts and fixtures.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 3 2012-01-01 2012-01-01 false Metal halide lamp ballasts and fixtures. 429.54 Section 429.54 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION CERTIFICATION, COMPLIANCE, AND ENFORCEMENT FOR... and tested to ensure that: (i) Any represented value of estimated energy efficiency calculated as the...

10 CFR 429.54 - Metal halide lamp ballasts and fixtures.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 3 2013-01-01 2013-01-01 false Metal halide lamp ballasts and fixtures. 429.54 Section 429.54 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION CERTIFICATION, COMPLIANCE, AND ENFORCEMENT FOR... and tested to ensure that: (i) Any represented value of estimated energy efficiency calculated as the...

10 CFR 850.10 - Development and approval of the CBDPP.

Code of Federal Regulations, 2010 CFR

2010-01-01

... DOE. (1) The responsible employer at a DOE facility must ensure that a CBDPP is prepared for the... approved or rejected by DOE earlier. (2) The responsible employer must furnish a copy of the approved CBDPP..., and affected workers or their designated representatives. (c) Update. The responsible employer must...

Distance Learning Conference Proceedings (Ruidoso, New Mexico, October 1989).

ERIC Educational Resources Information Center

Alexander, John B., Comp.; And Others

The declining emphasis on science, mathematics, and engineering education in the United States together with the necessity of the nation to ensure a continuous supply of trained practitioners in those fields was the consideration that prompted the conference reported in this document. The conference participants--who were representatives of the…

29 CFR 1960.6 - Designation of agency safety and health officials.

Code of Federal Regulations, 2011 CFR

2011-07-01

... responsibility to represent effectively the interest and support of the agency head in the management and... Order 12196, and this part; (2) An organization, including provision for the designation of safety and... safety and health program at all operational levels; (3) A set of procedures that ensures effective...

12 CFR 362.4 - Subsidiaries of insured State banks.

Code of Federal Regulations, 2011 CFR

2011-01-01

... practices, and/or to ensure that the activity is consistent with the purposes of Federal deposit insurance... Insurance Corporation if the subsidiary was engaged in the activity on or before September 30, 1991. (3) Majority-owned subsidiaries' ownership of equity investments that represent a control interest in a company...

Is U.S. climatic diversity well represented within the existing federal protection network?

Treesearch

Enric Batllori; Carol Miller; Marc-Andre Parisien; Sean A. Parks; Max A. Moritz

2014-01-01

Establishing protection networks to ensure that biodiversity and associated ecosystem services persist under changing environments is a major challenge for conservation planning. The potential consequences of altered climates for the structure and function of ecosystems necessitates new and complementary approaches be incorporated into traditional conservation plans....

Preventing Youth Violence and Aggression and Promoting Safety in Schools.

ERIC Educational Resources Information Center

Mulhern, Sean; And Others

Increased violence in schools, represented by possession of weapons, sexual or racial harassment, bullying, verbal intimidation, gang or cult activity, arson, or corporal punishment of students, for example, is a growing concern among students, educators, and communities. Since educators alone cannot ensure safety in schools, collaboration among…

10 CFR 429.20 - Residential clothes washers.

Code of Federal Regulations, 2013 CFR

2013-01-01

...). For standard-size residential clothes washers, a water factor (WF) in gallons per cycle per cubic feet... integrated modified energy factor (IMEF) in cu ft/kWh/cycle, the integrated water factor (IWF) in gal/cycle... randomly selected and tested to ensure that— (i) Any represented value of the water factor, integrated...

10 CFR 429.20 - Residential clothes washers.

Code of Federal Regulations, 2014 CFR

2014-01-01

...). For standard-size residential clothes washers, a water factor (WF) in gallons per cycle per cubic feet... integrated modified energy factor (IMEF) in cu ft/kWh/cycle, the integrated water factor (IWF) in gal/cycle... randomly selected and tested to ensure that— (i) Any represented value of the water factor, integrated...

4Bs or Not 4Bs: Bricks, Bytes, Brains, and Bandwidth

ERIC Educational Resources Information Center

Treat, Tod

2011-01-01

The effective integration of planning to include bricks, bytes, brains, and bandwidth (the 4Bs) represents an opportunity for community colleges to extend their capacity as knowledge-intensive organizations, coupling knowledge, technology, and learning. Integration is important to ensure that the interplay among organizations, agents within them,…

Presenters at NASP 2013-2016: Where Are the Practitioners?

ERIC Educational Resources Information Center

Aspiranti, Kathleen B.; Jackson, Carrie

2018-01-01

Although one of the main functions of school psychology research is to inform practice, there remains a large research-to-practice gap. Both practitioners and scholars are crucial in ensuring that research within the field is comprehensive and well represented. In the current study, presenter, contributor, and presenter affiliation demographics…

Emotional and Behavioral Problems Reported in Child Welfare over 3 Years

ERIC Educational Resources Information Center

McCrae, Julie S.

2009-01-01

Child welfare agencies are required to provide services that ensure that children receive adequate mental health care. This study provides a comprehensive view of the emotional and behavioral problems of children who are referred to child welfare services, using nationally representative data. Bivariate analyses compare rates by child…

75 FR 11633 - Proposed Collection; Comment Request for Form 720-CS

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-11

...: Representatives of the motor fuel industry, State governments, and the Federal government are working to ensure... of Respondents: 39,900. Estimated Time per Respondent: 5 hours, 15 minutes. Estimated Total Annual Burden Hours: 209,418. The following paragraph applies to all of the collections of information covered...

To Protect a Free Society: Maintaining Excellence in the Military.

ERIC Educational Resources Information Center

Smith, Dewitt C., Jr.

1985-01-01

A retired general reflects upon the distinctive traits of the American leader. The presence of the ROTC on many campuses ensures America of a military leadership that is varied in origin and perspective, widely representative, and broadly educated in the liberal values and traditions of society. (MLW)

45 CFR 84.44 - Academic adjustments.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 45 Public Welfare 1 2010-10-01 2010-10-01 false Academic adjustments. 84.44 Section 84.44 Public..., manual, or speaking skills as will best ensure that the results of the evaluation represents the student... speaking skills (except where such skills are the factors that the test purports to measure). (d) Auxiliary...

45 CFR 84.44 - Academic adjustments.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 45 Public Welfare 1 2011-10-01 2011-10-01 false Academic adjustments. 84.44 Section 84.44 Public..., manual, or speaking skills as will best ensure that the results of the evaluation represents the student... speaking skills (except where such skills are the factors that the test purports to measure). (d) Auxiliary...

A Guide to Public Engagement and School Finance Litigation

ERIC Educational Resources Information Center

Zhang, Julia

2008-01-01

Lawsuits challenging the constitutionality of public education funding systems are currently underway in 21 states. Litigation represents an opportunity to restructure the ways in which public education is financed, expanded, and delivered to children across the country. Public engagement plays a uniquely important role to ensure real improvement…

U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR COLLECTION, STORAGE, AND SHIPMENT OF URINE SAMPLES FOR METALS AND PESTICIDES ANALYSIS (UA-F-20.1)

EPA Science Inventory

The purpose of this SOP is to guide the collection, storage, and shipment of urine samples collected. This SOP provides a brief description of sample, collection, preservation, storage, shipping, and custody procedures. This procedure was followed to ensure consistent data retri...

U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR COLLECTION, STORAGE, AND SHIPMENT OF BLOOD SAMPLES FOR SELECTED METALS, PESTICIDES, AND VOCS (UA-F-19.1)

EPA Science Inventory

The purpose of this SOP is to guide the collection, storage, and shipment of blood samples collected. This SOP provides a brief description of sample collection, preservation, storage, and custody procedures. This procedure was followed to ensure consistent data retrieval during...

U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR FIELD COLLECTION OF YARD COMPOSITE SOIL SAMPLES (UA-F-5.1)

EPA Science Inventory

The purpose of this SOP is to establish a uniform procedure for the collection of yard composite soil samples in the field. This procedure was followed to ensure consistent and reliable collection of outdoor soil samples during the Arizona NHEXAS project and the Border study. Ke...

Underground Test Area Calendar Year 2015 Annual Sampling Analysis Report Nevada National Security Site, Nevada, Revision 0

DOE Office of Scientific and Technical Information (OSTI.GOV)

Farnham, Irene; Rehfeldt, Ken

This report presents the analytical data for the 2015 calendar year (CY) (January 1 through December 31, 2015) and an evaluation of the data to ensure that the Sampling Plan’s objectives are met. Special investigations that took place in 2015 that are relevant to the Sampling Plan are also presented.

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR COLLECTION, STORAGE AND SHIPMENT OF BLOOD SAMPLES FOR SELECTED METALS AND VOCS (UA-F-19.1)

EPA Science Inventory

The purpose of this SOP is to guide the collection, storage, and shipment of blood samples collected for the NHEXAS Arizona project. This SOP provides a brief description of sample collection, preservation, storage, and custody procedures. This procedure was followed to ensure c...

42 CFR 431.972 - Claims sampling procedures.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Estimating Improper Payments in Medicaid and CHIP § 431.972 Claims sampling procedures. (a) Claims universe. (1) The PERM claims universe includes payments that were originally paid (paid claims) and for which... must establish controls to ensure FFS and managed care universes are accurate and complete, including...

42 CFR 431.972 - Claims sampling procedures.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Estimating Improper Payments in Medicaid and CHIP § 431.972 Claims sampling procedures. (a) Claims universe. (1) The PERM claims universe includes payments that were originally paid (paid claims) and for which... must establish controls to ensure FFS and managed care universes are accurate and complete, including...

42 CFR 431.972 - Claims sampling procedures.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Estimating Improper Payments in Medicaid and CHIP § 431.972 Claims sampling procedures. (a) Claims universe. (1) The PERM claims universe includes payments that were originally paid (paid claims) and for which... must establish controls to ensure FFS and managed care universes are accurate and complete, including...

RADIOANALYTICAL AND MIXED WASTE ANALYTICAL SUPPORT FOR STATES, REGIONS, AND OTHER FEDERAL AGENCIES

EPA Science Inventory

Provide technical advice and support to Regions and other Federal Agencies on types of analyses, proper sampling, preservation, shipping procedures, and detection limits for samples for radionuclides and stable metals. Provide in-house data review and validation to ensure the qua...

Microbiota and Particulate Matter Assessment in Portuguese Optical Shops Providing Contact Lens Services

PubMed Central

Viegas, Carla; Faria, Tiago; Pacífico, Cátia; Dos Santos, Mateus; Monteiro, Ana; Lança, Carla; Carolino, Elisabete; Viegas, Susana; Cabo Verde, Sandra

2017-01-01

The aim of this work was to assess the microbiota (fungi and bacteria) and particulate matter in optical shops, contributing to a specific protocol to ensure a proper assessment. Air samples were collected through an impaction method. Surface and equipment swab samples were also collected side-by-side. Measurements of particulate matter were performed using portable direct-reading equipment. A walkthrough survey and checklist was also applied in each shop. Regarding air sampling, eight of the 13 shops analysed were above the legal requirement and 10 from the 26 surfaces samples were overloaded. In three out of the 13 shops fungal contamination in the analysed equipment was not detected. The bacteria air load was above the threshold in one of the 13 analysed shops. However, bacterial counts were detected in all sampled equipment. Fungi and bacteria air load suggested to be influencing all of the other surface and equipment samples. These results reinforce the need to improve air quality, not only to comply with the legal requirements, but also to ensure proper hygienic conditions. Public health intervention is needed to assure the quality and safety of the rooms and equipment in optical shops that perform health interventions in patients. PMID:28505144

Development of a Brief Version of the Pathological Narcissism Inventory

PubMed Central

Schoenleber, Michelle; Roche, Michael J.; Wetzel, Eunike; Pincus, Aaron L.; Roberts, Brent W.

2015-01-01

With theoretical and empirical interest in narcissism growing, there is a need for brief measures of pathological narcissism that can be used more practically while assessing the construct comprehensively. Data from four samples (total n = 3,851) collected across two separate research groups and representing undergraduate, community, and clinical populations was used to establish the reliability, validity, and utility of the Brief-Pathological Narcissism Inventory (B-PNI). Item response theory and confirmatory factor analyses were used to determine the best-performing 28 items from the original PNI and ensure that the B-PNI exhibited a factor structure consistent with the original PNI. Items were retained for all seven pathological narcissism facet scales. Additional results also support the criterion validity of the B-PNI, suggesting it can be used in place of the original PNI to assess the various facets of pathological narcissism effectively and without loss of information, which may enhance the ability of researchers to investigate pathological narcissism in future work. PMID:26011478

Development of a brief version of the Pathological Narcissism Inventory.

PubMed

Schoenleber, Michelle; Roche, Michael J; Wetzel, Eunike; Pincus, Aaron L; Roberts, Brent W

2015-12-01

With theoretical and empirical interest in narcissism growing, there is a need for brief measures of pathological narcissism that can be used more practically while assessing the construct comprehensively. Data from four samples (total N = 3,851) collected across two separate research groups and representing undergraduate, community, and clinical populations were used to establish the reliability, validity, and utility of the Brief-Pathological Narcissism Inventory (B-PNI). Item response theory and confirmatory factor analyses were used to determine the best-performing 28 items from the original PNI and ensure that the B-PNI exhibited a factor structure consistent with the original PNI. Items were retained for all seven pathological narcissism facet scales. Additional results also support the criterion validity of the B-PNI, suggesting that it can be used in place of the original PNI to assess the various facets of pathological narcissism effectively and without loss of information, which may enhance the ability of researchers to investigate pathological narcissism in future work. (c) 2015 APA, all rights reserved).

Prioritizing therapeutic targets using patient-derived xenograft models

PubMed Central

Lodhia, K.A; Hadley, A; Haluska, P; Scott, C.L

2015-01-01

Effective systemic treatment of cancer relies on the delivery of agents with optimal therapeutic potential. The molecular age of medicine has provided genomic tools that can identify a large number of potential therapeutic targets in individual patients, heralding the promise of personalized treatment. However, determining which potential targets actually drive tumor growth and should be prioritized for therapy is challenging. Indeed, reliable molecular matches of target and therapeutic agent have been stringently validated in the clinic for only a small number of targets. Patient-derived xenografts (PDX) are tumor models developed in immunocompromised mice using tumor procured directly from the patient. As patient surrogates, PDX models represent a powerful tool for addressing individualized therapy. Challenges include humanizing the immune system of PDX models and ensuring high quality molecular annotation, in order to maximise insights for the clinic. Importantly, PDX can be sampled repeatedly and in parallel, to reveal clonal evolution, which may predict mechanisms of drug resistance and inform therapeutic strategy design. PMID:25783201

Blind compressed sensing image reconstruction based on alternating direction method

NASA Astrophysics Data System (ADS)

Liu, Qinan; Guo, Shuxu

2018-04-01

In order to solve the problem of how to reconstruct the original image under the condition of unknown sparse basis, this paper proposes an image reconstruction method based on blind compressed sensing model. In this model, the image signal is regarded as the product of a sparse coefficient matrix and a dictionary matrix. Based on the existing blind compressed sensing theory, the optimal solution is solved by the alternative minimization method. The proposed method solves the problem that the sparse basis in compressed sensing is difficult to represent, which restrains the noise and improves the quality of reconstructed image. This method ensures that the blind compressed sensing theory has a unique solution and can recover the reconstructed original image signal from a complex environment with a stronger self-adaptability. The experimental results show that the image reconstruction algorithm based on blind compressed sensing proposed in this paper can recover high quality image signals under the condition of under-sampling.

Discovery, identification and mitigation of isobaric sulfate metabolite interference to a phosphate prodrug in LC-MS/MS bioanalysis: Critical role of method development in ensuring assay quality.

PubMed

Yuan, Long; Ji, Qin C

2018-06-05

Metabolite interferences represent a major risk of inaccurate quantification when using LC-MS/MS bioanalytical assays. During LC-MS/MS bioanalysis of BMS-919194, a phosphate ester prodrug, in plasma samples from rat and monkey GLP toxicology studies, an unknown peak was detected in the MRM channel of the prodrug. This peak was not observed in previous discovery toxicology studies, in which a fast gradient LC-MS/MS method was used. We found out that this unknown peak would co-elute with the prodrug peak when the discovery method was used, therefore, causing significant overestimation of the exposure of the prodrug in the discovery toxicology studies. To understand the nature of this interfering peak and its impact to bioanalytical assay, we further investigated its formation and identification. The interfering compound and the prodrug were found to be isobaric and to have the same major product ions in electrospray ionization positive mode, thus, could not be differentiated using a triple quadrupole mass spectrometer. By using high-resolution mass spectrometry (HRMS), the interfering metabolite was successfully identified to be an isobaric sulfate metabolite of BMS-919194. To the best of our knowledge, this is the first report that a phosphate prodrug was metabolized in vivo to an isobaric sulfate metabolite, and this metabolite caused significant interference to the analysis of the prodrug. This work demonstrated the presence of the interference risk from isobaric sulfate metabolites to the bioanalysis of phosphate prodrugs in real samples. It is critical to evaluate and mitigate potential metabolite interferences during method development, therefore, minimize the related bioanalytical risks and ensure assay quality. Our work also showed the unique advantages of HRMS in identifying potential metabolite interference during LC-MS/MS bioanalysis. Copyright © 2018 Elsevier B.V. All rights reserved.

Determining anthropometric variables as a tool in the preparation of furniture and designs of interior spaces: the case of children 6 to 11 years old of Vicosa, State of Minas Gerais, Brazil.

PubMed

Zanuncio, Sharinna Venturim; Mafra, Simone Caldas Tavares; Antônio, Carlos Emílio Barbosa; Filho, Jugurta Lisboa; Vidigal Guimarães, Elza Maria; da Silva, Vania Eugênia; de Souza, Amaury Paulo; Minette, Luciano José

2012-01-01

The adequacy of facilities and the individual securities in their different age groups is importance to ensure greater functionality to them, allowing full development of daily activities. For this to occur more efficiently it is necessary the use of ergonomics which can ensure more comfort and safety for end users of products and spaces. The present study aimed to measure body dimensions of a representative sample of children aged 6 to 11 years old, children of graduate and pos graduate students, faculty and staff of the Federal University of Vicosa and also residents of the city of Vicosa, State of Minas Gerais, Brazil, coming from different municipalities of State of Minas Gerais, to organize a database that will provide the furniture industry, anthropometric variables more appropriate to design products for both the leisure activities, and for the school sector. To realize this research we used the methodology proposed by the authors Panero and Zelnik, based on samples distributed in six age groups, and providing a measurement of 10 variables. By applying the methodology to the field was possible to compare the observed data, with the tables of the aforementioned authors. The main results revealed a significant variation of the 10 variables analyzed, and it is believed that this variation could lead to possible flaws in the designs of products that use the data from these authors. The completion of the study provided data on Vicosa considered more appropriate for the design of products and environments for the population of the study, considering age and region, of Brazil (State of Minas Gerais) and it is believed that the future may expand to the Brazilian population, with the progress of study of this nature.

Cataract in children attending schools for the blind and resource centers in eastern Africa.

PubMed

Msukwa, Gerald; Njuguna, Margaret; Tumwesigye, Cillasy; Shilio, Bernadeth; Courtright, Paul; Lewallen, Susan

2009-05-01

The aim of this study was to describe results of a representative sample of children who have undergone cataract surgery in schools for the blind in 4 African countries. Cross-sectional study. Children enrolled at schools for the blind in Kenya, Malawi, Tanzania, and Uganda. We used a population-proportional-to-size methodology to select a representative sample of schools for the blind and annexes and included all the children attending the selected schools. Trained teams using standardized examination methods and a modified World Health Organization form examined the children. The form was modified specifically to collect information on outcomes of cataract surgery. Operative status and postoperative visual acuity. Of 1062 children examined, 196 (18%) had undergone cataract surgery or had cataract as the major cause of visual impairment; 140 (71%) had bilateral surgery, 24 (12%) had unilateral surgery, and 32 (16%) had not had surgery. Of operated eyes, 118 (41%) had visual acuity > or =20/200. Intraocular lenses were implanted in 65% of the operated eyes. Eyes with intraocular lens were more likely to have better vision than those without (P for trend = 0.04). Amblyopia was the most common cause of poor visual acuity in children who had undergone cataract surgery. The number of children in the schools who receive cataract surgery has increased greatly since 1995. The high rate of amblyopia highlights the critical need for programs to find children earlier and to ensure adequate follow-up after surgery. Without such programs, the value of training pediatric surgeons will not be fully realized. The authors have no proprietary or commercial interest in any materials discussed in this article.

Missed opportunities and barriers for vaccination: a descriptive analysis of private and public health facilities in four African countries.

PubMed

Olorunsaiye, Comfort Zuyeali; Langhamer, Margaret Shaw; Wallace, Aaron Stuart; Watkins, Margaret Lyons

2017-01-01

Missed opportunities and barriers to vaccination limit progress toward achieving high immunization coverage and other global immunization goals. Little is known about vaccination practices contributing to missed opportunities and barriers among private healthcare providers in Africa. Service Provision Assessments (SPA) of representative samples of health facilities in four African countries (Kenya, Tanzania, Senegal, Malawi) in 2010-2015 were used to describe missed opportunities and barriers for vaccination in public, private for-profit, private not-for-profit and faith-based facilities. Data included vaccination practices, observations during sick child and antenatal visits, and exit interviews following sick child visits. Data from 3,219 health facilities, 11,613 sick child visits and 8,698 antenatal visits were included. A smaller proportion of for-profit facilities offered child vaccination services (country range, 25-37%) than did public facilities (range, 90-96%). The proportion of facilities offering pentavalent vaccine (diphtheria, pertussis, tetanus, hepatitis B and Haemophilus influenza type b antigens) daily ranged 0-77% across countries and facility types. Less than 33% of for-profit facilities in any country offered measles vaccination daily. A minority of public or private providers assessed the child's vaccination status during a sick child visit (range by country and facility type, 14-44%), or offered tetanus toxoid during antenatal visits (range, 19-51%). Very few providers discussed the importance of newborn vaccination. Substantial missed opportunities for, and barriers to, vaccination were identified across this representative sample of health facilities in four African countries. Strategies are needed to ensure that private and public providers implement practices to minimize barriers and missed opportunities for vaccination.

Bat assemblages in conservation areas of a metropolitan region in Southeastern Brazil, including an important karst habitat.

PubMed

Talamoni, S A; Coelho, D A; Dias-Silva, L H; Amaral, A S

2013-05-01

Species richness and abundance of bats were studied in four nature reserves, including a karst area which has many potential rocky shelters for bats, such as caves and rock crevices. The reserves were located in the greater Belo Horizonte metropolitan area, one of the most populated regions of Brazil, within the Atlantic Forest, and Cerrado (Brazilian savanna) ecological domains. Bats were sampled using mist-nets and, in the karst area, also by active searches in shelters. A total of 1,599 bats were captured representing 30 species belonging to four families. There was little similarity among the four chiropteran faunas. The greatest species richness was found in the karst area with 22 species recorded whereas richness estimates in the other areas indicated the need for further studies. Two hundred and sixty-five individuals of 14 species were captured from 56 shelters. Most of the shelters were frequently used for diurnal roosts, and all the bats found belonged to the Phyllostomidae, with the exception of Myotis nigricans (Vespertilionidae), Nyctinomops laticaudatus (Molossidae) and Peropteryx macrotis (Emballonuridae). The sanguinivorous Desmodus rotundus was the most common species in the shelters. The results of this study demonstrate the importance of maintaining multiple protected areas to ensure a representative fauna of bats in a region characterized by a vegetation transition zone and with intense economic activity and high environmental impact. This study also demonstrates the importance of rock shelters for maintaining local bat richness and the importance of active searches for bats in their diurnal roosts for a more thorough sampling of the bat fauna at a given locality.

Missed opportunities and barriers for vaccination: a descriptive analysis of private and public health facilities in four African countries

PubMed Central

Olorunsaiye, Comfort Zuyeali; Langhamer, Margaret Shaw; Wallace, Aaron Stuart; Watkins, Margaret Lyons

2017-01-01

Introduction Missed opportunities and barriers to vaccination limit progress toward achieving high immunization coverage and other global immunization goals. Little is known about vaccination practices contributing to missed opportunities and barriers among private healthcare providers in Africa. Methods Service Provision Assessments (SPA) of representative samples of health facilities in four African countries (Kenya, Tanzania, Senegal, Malawi) in 2010-2015 were used to describe missed opportunities and barriers for vaccination in public, private for-profit, private not-for-profit and faith-based facilities. Data included vaccination practices, observations during sick child and antenatal visits, and exit interviews following sick child visits. Results Data from 3,219 health facilities, 11,613 sick child visits and 8,698 antenatal visits were included. A smaller proportion of for-profit facilities offered child vaccination services (country range, 25-37%) than did public facilities (range, 90-96%). The proportion of facilities offering pentavalent vaccine (diphtheria, pertussis, tetanus, hepatitis B and Haemophilus influenza type b antigens) daily ranged 0-77% across countries and facility types. Less than 33% of for-profit facilities in any country offered measles vaccination daily. A minority of public or private providers assessed the child’s vaccination status during a sick child visit (range by country and facility type, 14-44%), or offered tetanus toxoid during antenatal visits (range, 19-51%). Very few providers discussed the importance of newborn vaccination. Conclusion Substantial missed opportunities for, and barriers to, vaccination were identified across this representative sample of health facilities in four African countries. Strategies are needed to ensure that private and public providers implement practices to minimize barriers and missed opportunities for vaccination. PMID:29296141

Hollow-fiber flow field-flow fractionation with multi-angle laser scattering detection for aggregation studies of therapeutic proteins.

PubMed

Reschiglian, P; Roda, B; Zattoni, A; Tanase, M; Marassi, V; Serani, S

2014-02-01

The rapid development of protein-based pharmaceuticals highlights the need for robust analytical methods to ensure their quality and stability. Among proteins used in pharmaceutical applications, an important and ever increasing role is represented by monoclonal antibodies and large proteins, which are often modified to enhance their activity or stability when used as drugs. The bioactivity and the stability of those proteins are closely related to the maintenance of their complex structure, which however are influenced by many external factors that can cause degradation and/or aggregation. The presence of aggregates in these drugs could reduce their bioactivity and bioavailability, and induce immunogenicity. The choice of the proper analytical method for the analysis of aggregates is fundamental to understand their (size) dimensional range, their amount, and if they are present in the sample as generated by an aggregation or as an artifact due to the method itself. Size exclusion chromatography is one of the most important techniques for the quality control of pharmaceutical proteins; however, its application is limited to relatively low molar mass aggregates. Among the techniques for the size characterization of proteins, field-flow fractionation (FFF) represents a competitive choice because of its soft mechanism due to the absence of a stationary phase and application in a broader size range, from nanometer- to micrometer-sized analytes. In this paper, the microcolumn variant of FFF, the hollow-fiber flow FFF, was online coupled with multi-angle light scattering, and a method for the characterization of aggregates with high reproducibility and low limit of detection was demonstrated employing an avidin derivate as sample model.

Solid waste composition analysis and recycling evaluation: Zaatari Syrian Refugees Camp, Jordan.

PubMed

Saidan, Motasem N; Drais, Ammar Abu; Al-Manaseer, Ehab

2017-03-01

There is a need for Municipal Solid Waste (MSW) stream characterization and composition analysis to allow for an accurate estimation of its recycling potential and for effective management of the entire system. Recycling provides employment and a livelihood for vulnerable social groups such as refugees. The aim of this paper is to determine the composition of MSW in Zaatari Syrian Refugee Camp, where approximately 430,000 Syrian refugees have passed through the camp. The representative waste samples and analysis included household waste and commercial waste produced by the refugees in the selected districts in Zaatari. The waste sampling was performed in 2015 over two seasons to ensure that the seasonal fluctuations in the composition of the waste stream are taken into consideration. Hand sorting was used for classifying the collected wastes into the categories and subcategories. The organic waste represents the main waste category with 53% of the total MSW, while plastics, textile, and paper and cardboard are 12.85%, 10.22% and 9%, respectively. Moreover, the MSW composition percentage in Zaatari Camp is similar to that in municipalities in Jordan with slight disparity. The potential recyclable materials market has been investigated in this study. Plastics and paper and cardboard have significant potential to be separated and collected for recycling purposes. Financial revenues of potential recyclables have been analyzed based on local prices. Recycling model in the camp is also proposed based on the present study findings. Consequently, these results should be taken as a baseline for all Syrian refugees camps in the Middle East, as well as, in Europe. Copyright © 2016 Elsevier Ltd. All rights reserved.

On the Representativeness of Behavior Observation Samples in Classrooms

ERIC Educational Resources Information Center

Tiger, Jeffrey H.; Miller, Sarah J.; Mevers, Joanna Lomas; Mintz, Joslyn Cynkus; Scheithauer, Mindy C.; Alvarez, Jessica

2013-01-01

School consultants who rely on direct observation typically conduct observational samples (e.g., 1 30-min observation per day) with the hopes that the sample is representative of performance during the remainder of the day, but the representativeness of these samples is unclear. In the current study, we recorded the problem behavior of 3 referred…

Report: Independent Environmental Sampling Shows Some Properties Designated by EPA as Available for Use Had Some Contamination

EPA Pesticide Factsheets

Report #15-P-0221, July 21, 2015. Some OIG sampling results showed contamination was still present at sites designated by the EPA as ready for reuse. This was unexpected and could signal a need to implement changes to ensure human health protection.

76 FR 13125 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-10

... of monthly retail sales, end-of-month merchandise inventories, and quarterly e-commerce sales of... the GDP. Retail e-commerce sales are estimated from the same sample used in the Monthly Retail Trade... ensure that retail firms selected in the Monthly Retail Trade Survey sample engaged in e- commerce are...

40 CFR 86.1310-2007 - Exhaust gas sampling and analytical system for gaseous emissions from heavy-duty diesel-fueled...

Code of Federal Regulations, 2010 CFR

2010-07-01

... reference pursuant to § 86.1(b)(2). As an alternative, the manufacturer may choose one of the options set... engineering judgment shall dictate the use of mixing plates and mixing orifices to ensure a well-mixed sample...

40 CFR 86.1310-2007 - Exhaust gas sampling and analytical system for gaseous emissions from heavy-duty diesel-fueled...

Code of Federal Regulations, 2012 CFR

2012-07-01

... reference pursuant to § 86.1(b)(2). As an alternative, the manufacturer may choose one of the options set... engineering judgment shall dictate the use of mixing plates and mixing orifices to ensure a well-mixed sample...

40 CFR 86.1310-2007 - Exhaust gas sampling and analytical system for gaseous emissions from heavy-duty diesel-fueled...

Code of Federal Regulations, 2011 CFR

2011-07-01

... reference pursuant to § 86.1(b)(2). As an alternative, the manufacturer may choose one of the options set... engineering judgment shall dictate the use of mixing plates and mixing orifices to ensure a well-mixed sample...

40 CFR 86.1310-2007 - Exhaust gas sampling and analytical system for gaseous emissions from heavy-duty diesel-fueled...

Code of Federal Regulations, 2013 CFR

2013-07-01

... reference pursuant to § 86.1(b)(2). As an alternative, the manufacturer may choose one of the options set... engineering judgment shall dictate the use of mixing plates and mixing orifices to ensure a well-mixed sample...

Exploring geo-tagged photos for land cover validation with deep learning

NASA Astrophysics Data System (ADS)

Xing, Hanfa; Meng, Yuan; Wang, Zixuan; Fan, Kaixuan; Hou, Dongyang

2018-07-01

Land cover validation plays an important role in the process of generating and distributing land cover thematic maps, which is usually implemented by high cost of sample interpretation with remotely sensed images or field survey. With an increasing availability of geo-tagged landscape photos, the automatic photo recognition methodologies, e.g., deep learning, can be effectively utilised for land cover applications. However, they have hardly been utilised in validation processes, as challenges remain in sample selection and classification for highly heterogeneous photos. This study proposed an approach to employ geo-tagged photos for land cover validation by using the deep learning technology. The approach first identified photos automatically based on the VGG-16 network. Then, samples for validation were selected and further classified by considering photos distribution and classification probabilities. The implementations were conducted for the validation of the GlobeLand30 land cover product in a heterogeneous area, western California. Experimental results represented promises in land cover validation, given that GlobeLand30 showed an overall accuracy of 83.80% with classified samples, which was close to the validation result of 80.45% based on visual interpretation. Additionally, the performances of deep learning based on ResNet-50 and AlexNet were also quantified, revealing no substantial differences in final validation results. The proposed approach ensures geo-tagged photo quality, and supports the sample classification strategy by considering photo distribution, with accuracy improvement from 72.07% to 79.33% compared with solely considering the single nearest photo. Consequently, the presented approach proves the feasibility of deep learning technology on land cover information identification of geo-tagged photos, and has a great potential to support and improve the efficiency of land cover validation.

Assessment and modeling of groundwater quality using WQI and GIS in Upper Egypt area.

PubMed

Rabeiy, Ragab ElSayed

2017-04-04

The continuous growth and development of population need more fresh water for drinking, irrigation, and domestic in arid countries like Egypt. Evaluation the quality of groundwater is an essential study to ensure its suitability for different purposes. In this study, 812 groundwater samples were taken within the middle area of Upper Egypt (Sohag Governorate) to assess the quality of groundwater for drinking and irrigation purposes. Eleven water parameters were analyzed at each groundwater sample (Na + , K + , Ca 2+ , Mg 2+ , HCO 3 - SO 4 2- , Fe 2+ , Mn 2+ , Cl - , electrical conductivity, and pH) to exploit them in water quality evaluation. A classical statistics were applied for the raw data to examine the distribution of physicochemical parameters in the investigated area. The relationship between groundwater parameters was tested using the correlation coefficient where a strong relationship was found between several water parameters such as Ca 2+ and Cl - . Water quality index (WQI) is a mathematical model used to transform many water parameters into a single indicator value which represents the water quality level. Results of WQI showed that 20% of groundwater samples are excellent, 75% are good for drinking, and 7% are very poor water while only 1% of samples are unsuitable for drinking. To test the suitability of groundwater for irrigation, three indices are used; they are sodium adsorption ration (SAR), sodium percentage (Na%), and permeability index (PI). For irrigation suitability, the study proved that most sampling sites are suitable while less than 3% are unsuitable for irrigation. The spatial distribution of the estimated values of WQI, SAR, Na%, PI, and each groundwater parameter was spatially modeled using GIS.

Long-term room temperature preservation of corpse soft tissue: an approach for tissue sample storage

PubMed Central

2011-01-01

Background Disaster victim identification (DVI) represents one of the most difficult challenges in forensic sciences, and subsequent DNA typing is essential. Collected samples for DNA-based human identification are usually stored at low temperature to halt the degradation processes of human remains. We have developed a simple and reliable procedure for soft tissue storage and preservation for DNA extraction. It ensures high quality DNA suitable for PCR-based DNA typing after at least 1 year of room temperature storage. Methods Fragments of human psoas muscle were exposed to three different environmental conditions for diverse time periods at room temperature. Storage conditions included: (a) a preserving medium consisting of solid sodium chloride (salt), (b) no additional substances and (c) garden soil. DNA was extracted with proteinase K/SDS followed by organic solvent treatment and concentration by centrifugal filter devices. Quantification was carried out by real-time PCR using commercial kits. Short tandem repeat (STR) typing profiles were analysed with 'expert software'. Results DNA quantities recovered from samples stored in salt were similar up to the complete storage time and underscored the effectiveness of the preservation method. It was possible to reliably and accurately type different genetic systems including autosomal STRs and mitochondrial and Y-chromosome haplogroups. Autosomal STR typing quality was evaluated by expert software, denoting high quality profiles from DNA samples obtained from corpse tissue stored in salt for up to 365 days. Conclusions The procedure proposed herein is a cost efficient alternative for storage of human remains in challenging environmental areas, such as mass disaster locations, mass graves and exhumations. This technique should be considered as an additional method for sample storage when preservation of DNA integrity is required for PCR-based DNA typing. PMID:21846338

Detection and discrimination of orthopoxviruses using microarrays of immobilized oligonucleotides.

PubMed

Laassri, Majid; Chizhikov, Vladimir; Mikheev, Maxim; Shchelkunov, Sergei; Chumakov, Konstantin

2003-09-01

Variola virus (VARV), causing smallpox, is a potential biological weapon. Methods to detect VARV rapidly and to differentiate it from other viruses causing similar clinical syndromes are needed urgently. We have developed a new microarray-based method that detects simultaneously and discriminates four orthopoxvirus (OPV) species pathogenic for humans (variola, monkeypox, cowpox, and vaccinia viruses) and distinguishes them from chickenpox virus (varicella-zoster virus or VZV). The OPV gene C23L/B29R, encoding the CC-chemokine binding protein, was sequenced for 41 strains of seven species of orthopox viruses obtained from different geographical regions. Those C23L/B29R sequences and the ORF 62 sequences from 13 strains of VZV (selected from GenBank) were used to design oligonucleotide probes that were immobilized on an aldehyde-coated glass surface (a total of 57 probes). The microchip contained several unique 13-21 bases long oligonucleotide probes specific to each virus species to ensure redundancy and robustness of the assay. A region approximately 1100 bases long was amplified from samples of viral DNA and fluorescently labeled with Cy5-modified dNTPs, and single-stranded DNA was prepared by strand separation. Hybridization was carried out under plastic coverslips, resulting in a fluorescent pattern that was quantified using a confocal laser scanner. 49 known and blinded samples of OPV DNA, representing different OPV species, and two VZV strains were tested. The oligonucleotide microarray hybridization technique identified reliably and correctly all samples. This new procedure takes only 3 h, and it can be used for parallel testing of multiple samples.

Monitoring of Ritz modal generation

NASA Technical Reports Server (NTRS)

Chargin, Mladen; Butler, Thomas G.

1990-01-01

A scheme is proposed to monitor the adequacy of a set of Ritz modes to represent a solution by comparing the quantity generated with certain properties involving the forcing function. In so doing an attempt was made to keep this algorithm lean and efficient, so that it will be economical to apply. Using this monitoring scheme during Ritz Mode generation will automatically ensure that the k Ritz modes theta k that are generated are adequate to represent both the spatial and temporal behavior of the structure when forced under the given transient condition defined by F(s,t).

Autonomous support for microorganism research in space

NASA Technical Reports Server (NTRS)

Fleet, Mary L.; Miller, Mark S.; Shipley, Derek, E.; Smith, Jeff D.

1992-01-01

A preliminary design for performing on orbit, autonomous research on microorganisms and cultured cells/tissues is presented. An understanding of gravity and its effects on cells is crucial for space exploration as well as for terrestrial applications. The payload is designed to be compatible with the Commercial Experiment Transporter (COMET) launch vehicle, an orbiter middeck locker interface, and with Space Station Freedom. Uplink/downlink capabilities and sample return through controlled reentry are available for all carriers. Autonomous testing activities are preprogrammed with in-flight reprogrammability. Sensors for monitoring temperature, pH, light, gravity levels, vibrations, and radiation are provided for environmental regulation and experimental data collection. Additional experimental data acquisition includes optical density measurement, microscopy, video, and film photography. On-board full data storage capabilities are provided. A fluid transfer mechanism is utilized for inoculation, sampling, and nutrient replenishment of experiment cultures. In addition to payload design, representative experiments were developed to ensure scientific objectives remained compatible with hardware capabilities. The project is defined to provide biological data pertinent to extended duration crewed space flight including crew health issues and development of a Controlled Ecological Life Support System (CELSS). In addition, opportunities are opened for investigations leading to commercial applications of space, such as pharmaceutical development, modeling of terrestrial diseases, and material processing.

Quality of antenatal care predicts retention in skilled birth attendance: a multilevel analysis of 28 African countries.

PubMed

Chukwuma, Adanna; Wosu, Adaeze C; Mbachu, Chinyere; Weze, Kelechi

2017-05-25

An effective continuum of maternal care ensures that mothers receive essential health packages from pre-pregnancy to delivery, and postnatally, reducing the risk of maternal death. However, across Africa, coverage of skilled birth attendance is lower than coverage for antenatal care, indicating mothers are not retained in the continuum between antenatal care and delivery. This paper explores predictors of retention of antenatal care clients in skilled birth attendance across Africa, including sociodemographic factors and quality of antenatal care received. We pooled nationally representative data from Demographic and Health Surveys conducted in 28 African countries between 2006 and 2015. For the 115,374 births in our sample, we estimated logistic multilevel models of retention in skilled birth attendance (SBA) among clients that received skilled antenatal care (ANC). Among ANC clients in the study sample, 66% received SBA. Adjusting for all demographic covariates and country indicators, the odds of retention in SBA were higher among ANC clients that had their blood pressure checked, received information about pregnancy complications, had blood tests conducted, received at least one tetanus injection, and had urine tests conducted. Higher quality of ANC predicts retention in SBA in Africa. Improving quality of skilled care received prenatally may increase client retention during delivery, reducing maternal mortality.

Autonomous support for microorganism research in space

NASA Technical Reports Server (NTRS)

Luttges, M. W.; Klaus, D. M.; Fleet, M. L.; Miller, M. S.; Shipley, D. E.; Smith, J. D.

1992-01-01

A preliminary design for performing on-orbit, autonomous research on microorganisms and cultured cells/tissues is presented. An understanding of gravity and its effects on cells is crucial for space exploration as well as for terrestrial applications. The payload is designed to be compatible with the COMmercial Experiment Transported (COMET) launch vehicle, an orbiter middeck locker interface, and with Space Station Freedom. Uplink/downlink capabilities and sample return through controlled reentry are available for all carriers. Autonomous testing activities are preprogrammed with inflight reprogrammability. Sensors for monitoring temperature, pH, light, gravity levels, vibration, and radiation are provided for environmental regulation and experimental data collection. Additional experiment data acquisition includes optical density measurement, microscopy, video, and file photography. Onboard full data storage capabilities are provided. A fluid transfer mechanism is utilized for inoculation, sampling, and nutrient replenishment of experiment cultures. In addition to payload design, representative experiments were developed to ensure scientific objectives remained compatible with hardware capabilities. The project is defined to provide biological data pertinent to extended duration crewed space flight including crew health issues and development of a Controlled Ecological Life Support System (CELSS). In addition, opportunities are opened for investigations leading to commercial applications of space, such as pharmaceutical development, modeling of terrestrial diseases, and material processing.

Sci—Thur PM: Imaging — 06: Canada's National Computed Tomography (CT) Survey

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wardlaw, GM; Martel, N; Blackler, W

2014-08-15

The value of computed tomography (CT) in medical imaging is reflected in its' increased use and availability since the early 1990's; however, given CT's relatively larger exposures (vs. planar x-ray) greater care must be taken to ensure that CT procedures are optimised in terms of providing the smallest dose possible while maintaining sufficient diagnostic image quality. The development of CT Diagnostic Reference Levels (DRLs) supports this process. DRLs have been suggested/supported by international/national bodies since the early 1990's and widely adopted elsewhere, but not on a national basis in Canada. Essentially, CT DRLs provide guidance on what is considered goodmore » practice for common CT exams, but require a representative sample of CT examination data to make any recommendations. Canada's National CT Survey project, in collaboration with provincial/territorial authorities, has collected a large national sample of CT practice data for 7 common examinations (with associated clinical indications) of both adult and pediatric patients. Following completion of data entry into a common database, a survey summary report and recommendations will be made on CT DRLs from this data. It is hoped that these can then be used by local regions to promote CT practice optimisation and support any dose reduction initiatives.« less

Do site-specific radiocarbon measurements reflect localized distributions of 14C in biota inhabiting a wetland with point contamination sources?

PubMed

Yankovich, T; King-Sharp, K J; Benz, M L; Carr, J; Killey, R W D; Beresford, N A; Wood, M D

2013-12-01

Duke Swamp is a wetland ecosystem that receives (14)C via a groundwater pathway originating from a waste management area on Atomic Energy Canada Limited's Chalk River Laboratories site. This groundwater reaches the surface of the swamp, resulting in relatively high (14)C levels over an area of 146 m(2). The objective of this study was to quantify (14)C concentrations in flora and fauna inhabiting areas of Duke Swamp over the gradient of (14)C activity concentrations in moss to determine whether (14)C specific activities in receptor biota reflect the localized nature of the groundwater source in the swamp. Representative receptor plants and animals, and corresponding air and soil samples were collected at six sites in Duke Swamp with (14)C specific activities in air that ranged from 1140 to 45,900 Bq/kg C. In general, it was found that specific activities of (14)C in biota tissues reflected those measured in environmental media collected from the same sampling site. The findings demonstrate that mosses could be used in monitoring programs to ensure protection of biota in areas with elevated (14)C, negating the need to capture and euthanize higher organisms. Copyright © 2013 Elsevier Ltd. All rights reserved.

The Influence of Maternal and Household Resources, and Parental Psychosocial Child Stimulation on Early Childhood Development: A Cross-Sectional Study of Children 36⁻59 Months in Honduras.

PubMed

Urke, Helga Bjørnøy; Contreras, Mariela; Matanda, Dennis Juma

2018-05-07

Optimal early childhood development (ECD) is currently jeopardized for more than 250 million children under five in low- and middle-income countries. The Sustainable Development Goals has called for a renewed emphasis on children’s wellbeing, encompassing a holistic approach that ensures nurturing care to facilitate optimal child development. In vulnerable contexts, the extent of a family’s available resources can influence a child’s potential of reaching its optimal development. Few studies have examined these relationships in low- and middle-income countries using nationally representative samples. The present paper explored the relationships between maternal and paternal psychosocial stimulation of the child as well as maternal and household resources and ECD among 2729 children 36⁻59 months old in Honduras. Data from the Demographic and Health Surveys conducted in 2011⁻2012 was used. Adjusted logistic regression analyses showed that maternal psychosocial stimulation was positively and significantly associated with ECD in the full, rural, and lowest wealth quintile samples. These findings underscore the importance of maternal engagement in facilitating ECD but also highlight the role of context when designing tailored interventions to improve ECD.

4D Infant Cortical Surface Atlas Construction using Spherical Patch-based Sparse Representation.

PubMed

Wu, Zhengwang; Li, Gang; Meng, Yu; Wang, Li; Lin, Weili; Shen, Dinggang

2017-09-01

The 4D infant cortical surface atlas with densely sampled time points is highly needed for neuroimaging analysis of early brain development. In this paper, we build the 4D infant cortical surface atlas firstly covering 6 postnatal years with 11 time points (i.e., 1, 3, 6, 9, 12, 18, 24, 36, 48, 60, and 72 months), based on 339 longitudinal MRI scans from 50 healthy infants. To build the 4D cortical surface atlas, first , we adopt a two-stage groupwise surface registration strategy to ensure both longitudinal consistency and unbiasedness. Second , instead of simply averaging over the co-registered surfaces, a spherical patch-based sparse representation is developed to overcome possible surface registration errors across different subjects. The central idea is that, for each local spherical patch in the atlas space, we build a dictionary, which includes the samples of current local patches and their spatially-neighboring patches of all co-registered surfaces, and then the current local patch in the atlas is sparsely represented using the built dictionary. Compared to the atlas built with the conventional methods, the 4D infant cortical surface atlas constructed by our method preserves more details of cortical folding patterns, thus leading to boosted accuracy in registration of new infant cortical surfaces.

Full-text publication of abstract-presented work in sport and exercise psychology

PubMed Central

Warden, Stuart

2018-01-01

Objectives Meetings promote information sharing, but do not enable full dissemination of details. A systematic search was conducted for abstracts presented at the 2010 and 2011 Association of Applied Sport Psychology Annual Conferences to determine the full-text dissemination rate of work presented in abstract form and investigate factors influencing this rate. Methods Systematic searches were sequentially conducted to determine whether the abstract-presented work had been published in full-text format in the 5 years following presentation. If a potential full-text publication was identified, information from the conference abstract (eg, results, number of participants in the sample(s), measurement tools used and so on) was compared with the full text to ensure the two entities represented the same body of work. Abstract factors of interest were assessed using logistic regression. Results Ninety-four out of 423 presented abstracts (22.2%) were published in full text. Odds of full-text publication increased if the abstract was from an international institution, presented in certain conference sections or presented as a lecture. Conclusion Those attending professional conferences should be cautious when translating data presented at conferences into their applied work because of the low rate of peer-reviewed and full-text publication of the information. PMID:29629187

40 CFR 86.210-08 - Exhaust gas sampling system; Diesel-cycle vehicles not requiring particulate emissions measurements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... detector (HFID) (375 °±20 °F (191 °±11 °C)) sample for total hydrocarbon (THC) analysis. The HFID sample... integrated measurement of diluted THC is required. Unless compensation for varying mass flow is made, a constant mass flow system must be used to ensure a proportional THC measurement. (2) For natural gas-fueled...

40 CFR 86.210-08 - Exhaust gas sampling system; Diesel-cycle vehicles not requiring particulate emissions measurements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... detector (HFID) (375 °±20 °F (191 °±11 °C)) sample for total hydrocarbon (THC) analysis. The HFID sample... integrated measurement of diluted THC is required. Unless compensation for varying mass flow is made, a constant mass flow system must be used to ensure a proportional THC measurement. (2) For natural gas-fueled...

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR PREPARATION OF FILTERS AND PUF FOR FIELD COLLECTION OF METALS AND PESTICIDES IN AIR (BCO-L-2.0)

EPA Science Inventory

The purpose of this SOP is to describe the procedures for pre-cleaning filters and polyurethane foam (PUF) plug prior to air sampling with these media. The sampling media are used for sampling indoor air, outdoor air, and personal air. This procedure was followed to ensure consi...

Automation practices in large molecule bioanalysis: recommendations from group L5 of the global bioanalytical consortium.

PubMed

Ahene, Ago; Calonder, Claudio; Davis, Scott; Kowalchick, Joseph; Nakamura, Takahiro; Nouri, Parya; Vostiar, Igor; Wang, Yang; Wang, Jin

2014-01-01

In recent years, the use of automated sample handling instrumentation has come to the forefront of bioanalytical analysis in order to ensure greater assay consistency and throughput. Since robotic systems are becoming part of everyday analytical procedures, the need for consistent guidance across the pharmaceutical industry has become increasingly important. Pre-existing regulations do not go into sufficient detail in regard to how to handle the use of robotic systems for use with analytical methods, especially large molecule bioanalysis. As a result, Global Bioanalytical Consortium (GBC) Group L5 has put forth specific recommendations for the validation, qualification, and use of robotic systems as part of large molecule bioanalytical analyses in the present white paper. The guidelines presented can be followed to ensure that there is a consistent, transparent methodology that will ensure that robotic systems can be effectively used and documented in a regulated bioanalytical laboratory setting. This will allow for consistent use of robotic sample handling instrumentation as part of large molecule bioanalysis across the globe.

Improvement of Productivity in TIG Welding Plant by Equipment Design in Orbit

NASA Astrophysics Data System (ADS)

Gnanavel, C.; Saravanan, R.; Chandrasekaran, M.; Jayakanth, J. J.

2017-03-01

Measurements and improvements are very indispensable task at all levels of management. Here some samples are, at operator level: Measuring operating parameters to ensure OEE (Overall Equipment Effectiveness) and measuring Q components performance to ensure quality, at supervisory level: measuring operator’s performance to ensure labour utility at managerial level: production and productivity measurements and at top level capital and capacity utilization. An often accepted statement is “Improvement is impossible without measurement”. Measurements often referred as observation. The case study was conducted at Government Boiler factory in India. The scientific approach followed for indentifying non value added activities. Personalised new equipment designed and installed to achieve productivity improvement of 85% for a day. The new equipment can serve 360o around its axis hence it simplified loading and unloading procedures as well as reduce their times and ensured effective space and time.

HIV testing and counseling: test providers' experiences of best practices.

PubMed

Myers, Ted; Worthington, Catherine; Haubrich, Dennis J; Ryder, Karen; Calzavara, Liviana

2003-08-01

Although education is central to HIV testing and counseling, little is known about the educational processes within the testing experience. This study investigated test providers' understandings of testing and counseling best practices. Interviews with a purposive sample of 24 test providers were thematically analyzed. Analysis revealed five best practices specific to HIV education and public health--ensuring information and education for HIV risk reduction, individualization of risk assessment, ensuring test results are given in person, providing information and referrals, and facilitating partner notification--and six practices not specific to HIV counseling relationship building. The latter were building trust and rapport; maintaining professional boundaries; ensuring a comfortable, safe environment; ensuring confidentiality; imparting nonjudgmntal attitude; and self-determination. The identified best practices demonstrated remarkable consistency across respondent subgroups. Although counseling was seen as largely educational and with a preventive focus, it included individualized messages based on assessments of risk, knowledge, and social and cultural characteristics.

Mentoring for Beginning Principals: Revisiting the Past or Preparing for the Future?

ERIC Educational Resources Information Center

Daresh, John C.

2007-01-01

This paper describes a study of mentoring programs for beginning principals in two different urban school districts. In both settings, the goal of mentoring was said to be support for instructional leadership behaviors by novice principals. This represents an alternative to traditional mentoring schemes designed solely to ensure that first year…

The Invisible Crisis: Connecting Schools with Homeless Families

ERIC Educational Resources Information Center

Shields, Carolyn M.; Warke, Amy

2010-01-01

Children and youth represent a growing proportion of the homeless population. Using the lens of transformative leadership, this multifamily case study explores the realities of homeless children, the challenges their families face, and the role of school leaders in ensuring that they receive a quality education. It recommends that leaders (1)…

Intelligence Testing and Cultural Diversity: Concerns, Cautions, and Considerations

ERIC Educational Resources Information Center

Ford, Donna Y.

2004-01-01

With so many unanswered questions and controversies regarding intelligence, testing in general, and testing diverse students in particular, what can educators in gifted education do to ensure that these students have access to and are represented in gifted education programs and services? In this monograph, the author examines test bias by first…

Electric deregulation: Defining and ensuring fair competition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kahn, A.E.

1998-04-01

Regulation has several important duties in the transition of the electricity industry to competition. But in fulfilling these responsibilities, regulators must refrain from policies pressed upon them by consumer representatives, on one side, and would-be rivals of utility companies, on the other, that would artificially handicap utilities and blunt the salutary forces of competition.

Legislative Priorities for the 111th Congress

ERIC Educational Resources Information Center

Community College Journal, 2009

2009-01-01

Lawmakers this year on Capitol Hill will weigh critical issues of importance to the nation's community colleges. The American Association of Community Colleges (AACC) and the Association of Community College Trustees (ACCT) will be hard at work to ensure the interests of two-year institutions are well represented. This article takes a brief look…

The Market Transformation of the System of Education in Russia

ERIC Educational Resources Information Center

Lukashenko, M.

2004-01-01

The market of educational services represents a socially oriented mechanism that ensures a constant link between the producers and consumers of economic goods in the sphere of education. For the purpose of guarding against negative effects of a purely market mechanism, it is supplemented by elements of state regulation. In other countries, the…

10 CFR 429.46 - Commercial clothes washers.

Code of Federal Regulations, 2014 CFR

2014-01-01

... the water factor in gallons per cubic feet per cycle (gal/cu ft/cycle) for units manufactured on or... randomly selected and tested to ensure that— (i) Any represented value of energy or water consumption or other measure of energy or water consumption of a basic model for which consumers would favor lower...

10 CFR 429.20 - Residential clothes washers.

Code of Federal Regulations, 2012 CFR

2012-01-01

... washers, a water factor (WF) in gallons per cycle per cubic feet (gal/cycle/cu ft). [76 FR 12451, Mar. 7... randomly selected and tested to ensure that— (i) Any represented value of the water factor, the estimated annual operating cost, the energy or water consumption, or other measure of energy or water consumption...

10 CFR 429.46 - Commercial clothes washers.

Code of Federal Regulations, 2012 CFR

2012-01-01

... the water factor in gallons per cubic feet per cycle (gal/cu ft/cycle) for units manufactured on or... randomly selected and tested to ensure that— (i) Any represented value of energy or water consumption or other measure of energy or water consumption of a basic model for which consumers would favor lower...

10 CFR 429.46 - Commercial clothes washers.

Code of Federal Regulations, 2013 CFR

2013-01-01

... the water factor in gallons per cubic feet per cycle (gal/cu ft/cycle) for units manufactured on or... randomly selected and tested to ensure that— (i) Any represented value of energy or water consumption or other measure of energy or water consumption of a basic model for which consumers would favor lower...

A Costing Model for Project-Based Information and Communication Technology Systems

ERIC Educational Resources Information Center

Stewart, Brian; Hrenewich, Dave

2009-01-01

A major difficulty facing IT departments is ensuring that the projects and activities to which information and communications technologies (ICT) resources are committed represent an effective, economic, and efficient use of those resources. This complex problem has no single answer. To determine effective use requires, at the least, a…

Uncertainty Representation in Visualizations of Learning Analytics for Learners: Current Approaches and Opportunities

ERIC Educational Resources Information Center

Demmans Epp, Carrie; Bull, Susan

2015-01-01

Adding uncertainty information to visualizations is becoming increasingly common across domains since its addition helps ensure that informed decisions are made. This work has shown the difficulty that is inherent to representing uncertainty. Moreover, the representation of uncertainty has yet to be thoroughly explored in educational domains even…

2012 NASA Cost Estimating Handbook Highlights

NASA Technical Reports Server (NTRS)

Rosenberg, Leigh; Stukes, Sherry

2012-01-01

The major goal is to ensure that appropriate policy is adopted and that best practices are being developed, communicated, and used across the Agency. -- Accomplished by engaging the NASA Cost Estimating Community representatives in the update. Scheduled to be complete by the end of FY 2012. Document has been through 3 detailed reviews across NASA.

Report to the Legislature: Child Welfare and Early Learning Partnerships

ERIC Educational Resources Information Center

Washington State Department of Early Learning, 2015

2015-01-01

House Bill 2519, sponsored by Representative Tana Senn, was passed during the 2014 legislative session and signed into law by Governor Jay Inslee. HB 2519 directs the Department of Early Learning (DEL) and the Department of Social and Health Services (DSHS) to jointly develop recommendations on methods to "better partner to ensure children…

Set Theory Applied to Uniquely Define the Inputs to Territorial Systems in Emergy Analyses

EPA Science Inventory

The language of set theory can be utilized to represent the emergy involved in all processes. In this paper we use set theory in an emergy evaluation to ensure an accurate representation of the inputs to territorial systems. We consider a generic territorial system and we describ...

Strategic Review of the Student Visa Program 2011. Go8 Response

ERIC Educational Resources Information Center

Group of Eight (NJ1), 2011

2011-01-01

The current and first independent review of Australia's student visa program by The Honorable Michael Knight AO is a welcome indication of the Government's recognition of the importance of international education to Australian society. It represents a crucial opportunity for reform to ensure Australian education institutions can compete for the…

40 CFR 1048.250 - What records must I keep and make available to EPA?

Code of Federal Regulations, 2010 CFR

2010-07-01

... construction, including its origin and buildup, steps you took to ensure that it represents production engines... (CONTINUED) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM NEW, LARGE NONROAD SPARK-IGNITION ENGINES Certifying Engine Families § 1048.250 What records must I keep and make available to EPA? (a) Send the...

She Has Great Spirit: Insight into Relationships between American Indian Dads and Daughters

ERIC Educational Resources Information Center

Reinhardt, Martin James; Perry Evenstad, Jan; Faircloth, Susan

2012-01-01

Data from this preliminary study, the American Indian--Dads and Daughters Survey, shed light on how American Indian fathers think and feel about their relationships with their daughters. Respondents represent an array of tribal affiliations, age, occupations, socioeconomic status, and geographical/geopolitical locations, helping to ensure that…

The English Language Learner Variable in Research: One Definition Is Not Enough

ERIC Educational Resources Information Center

Debossu, Stephanie C.

2015-01-01

Properly defining a population ensures that resources, such as funding and access, meet the needs, expectations, and intended outcomes for those represented. Ethical concerns arise when a target population, such as the English Language Learner population, is defined in numerous yet incomplete ways, and differently in research and in state policies…

Children's Rights in Education Research: From Aims to Outcomes

ERIC Educational Resources Information Center

Smith, Hilary A.; Haslett, Stephen J.

2017-01-01

One approach to children's rights in research is to adopt a methodology that focuses on eliciting children's perspectives. Ensuring representative participation from all children allows a diversity of contexts to be reflected in the results, and points to ways in which improvements can be made in specific settings. In cultural contexts where…

78 FR 40829 - Proposed Collection; Comment Request for Form 720-CS

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-08

...: Representatives of the motor fuel industry, state governments, and the Federal government are working to ensure...,900. Estimated Time per Respondent: 5 hours, 15 minutes. Estimated Total Annual Burden Hours: 209,418... relating to a collection of information must be retained as long as their contents may become material in...

75 FR 11637 - Proposed Collection; Comment Request for Form 720-TO

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-11

...: Representatives of the motor fuel industry, state governments, and the Federal government are working to ensure...,000. Estimated Time per Respondent: 4 hrs, 40 minutes. Estimated Total Annual Burden Hours: 2,347,020... relating to a collection of information must be retained as long as their contents may become material in...

75 FR 1800 - Notice of Proposed Information Collection for 1004-0102

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-13

... use and/or maintenance of their roads. These fees represent the BLM's share of road construction and... uses the report to ensure compliance with the timber sale contract, and to amortize road construction... reinstatement of an approval to collect information that documents the payment of road use fees for the use of...

Connecting to the World's Collections: Making the Case for the Conservation and Preservation of Our Cultural Heritage

ERIC Educational Resources Information Center

Stoner, Joyce Hill

2009-01-01

Sixty cultural heritage leaders from thirty-two countries, including representatives from Africa, Asia, the Middle East, South America, Australia, Europe, and North America gathered in October 2009 in Salzburg, Austria, to develop a series of practical recommendations to ensure optimal collections conservation worldwide. Convened at Schloss…

40 CFR 59.628 - What records must I keep and what reports must I send to EPA?

Code of Federal Regulations, 2010 CFR

2010-07-01

... unit, include all of the following: (i) The emission-data unit's construction, including its origin and buildup, steps you took to ensure that it represents production containers, any components you built... warranty report summarizing successful warranty claims by emission family under § 59.612, including the...

40 CFR 59.628 - What records must I keep and what reports must I send to EPA?

Code of Federal Regulations, 2012 CFR

2012-07-01

... unit, include all of the following: (i) The emission-data unit's construction, including its origin and buildup, steps you took to ensure that it represents production containers, any components you built... warranty report summarizing successful warranty claims by emission family under § 59.612, including the...

40 CFR 59.628 - What records must I keep and what reports must I send to EPA?

Code of Federal Regulations, 2014 CFR

2014-07-01

... unit, include all of the following: (i) The emission-data unit's construction, including its origin and buildup, steps you took to ensure that it represents production containers, any components you built... warranty report summarizing successful warranty claims by emission family under § 59.612, including the...

40 CFR 59.628 - What records must I keep and what reports must I send to EPA?

Code of Federal Regulations, 2013 CFR

2013-07-01

... unit, include all of the following: (i) The emission-data unit's construction, including its origin and buildup, steps you took to ensure that it represents production containers, any components you built... warranty report summarizing successful warranty claims by emission family under § 59.612, including the...

75 FR 14335 - Revisions to the Export Administration Regulations To Enhance U.S. Homeland Security: Addition of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-25

... identified by an interagency working group that is reviewing export control issues related to homeland security. The interagency working group is made up of representatives from the Departments of Commerce, Defense, Homeland Security and State. The purpose of the interagency working group is to ensure that...

42 CFR 416.50 - Condition for coverage-Patient rights.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Coverage § 416.50 Condition for coverage—Patient rights. The ASC must inform the patient or the patient's representative or surrogate of the patient's rights and must protect and promote the exercise of these rights, as... with verbal and written notice of the patient's rights in a language and manner that ensures the...

42 CFR 416.50 - Condition for coverage-Patient rights.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Coverage § 416.50 Condition for coverage—Patient rights. The ASC must inform the patient or the patient's representative or surrogate of the patient's rights and must protect and promote the exercise of these rights, as... with verbal and written notice of the patient's rights in a language and manner that ensures the...

From the Air: Rediscovering Our Raison D’etre

DTIC Science & Technology

2012-08-01

constraints and war weariness take their toll . Ensuring that the Air Force can serve the na- tion in its capacity as a strategic arm represents a... Lawson   and Jane Lawson , The First Air Campaign, August 1914–November 1918 (Conshohocken, PA:  Combined Books, 1996); and John H. Morrow Jr., The

3 CFR - Ensuring the Uniformed Services Employment and Reemployment Rights Act (USERRA) Protections

Code of Federal Regulations, 2013 CFR

2013-01-01

... representatives from: (i) the Department of Defense; (ii) the Department of Justice; (iii) the Department of Labor...) The Department of Defense and the Office of Personnel Management shall work together to improve data...) Protections Memorandum for the Heads of Executive Departments and Agencies The Uniformed Services Employment...

The future of the ogallala aquifer: We can measure it, but can we manage it?

USDA-ARS?s Scientific Manuscript database

Ensuring the availability of fresh water resources in sufficient quantity and quality to support human populations and surrounding ecosystems represents one of the grand challenges of our time. The Ogallala Aquifer, one of the largest freshwater aquifers in the world, is a prime example of the chall...

40 CFR 51.1009 - Reasonable further progress (RFP) requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... between the base year and the attainment year. (e) For a multi-State nonattainment area, the RFP plans for each State represented in the nonattainment area must demonstrate RFP on the basis of common multi... State in a multi-State nonattainment area must ensure that the sources within its boundaries comply with...

Safety Sufficiency for NextGen: Assessment of Selected Existing Safety Methods, Tools, Processes, and Regulations

NASA Technical Reports Server (NTRS)

Xu, Xidong; Ulrey, Mike L.; Brown, John A.; Mast, James; Lapis, Mary B.

2013-01-01

NextGen is a complex socio-technical system and, in many ways, it is expected to be more complex than the current system. It is vital to assess the safety impact of the NextGen elements (technologies, systems, and procedures) in a rigorous and systematic way and to ensure that they do not compromise safety. In this study, the NextGen elements in the form of Operational Improvements (OIs), Enablers, Research Activities, Development Activities, and Policy Issues were identified. The overall hazard situation in NextGen was outlined; a high-level hazard analysis was conducted with respect to multiple elements in a representative NextGen OI known as OI-0349 (Automation Support for Separation Management); and the hazards resulting from the highly dynamic complexity involved in an OI-0349 scenario were illustrated. A selected but representative set of the existing safety methods, tools, processes, and regulations was then reviewed and analyzed regarding whether they are sufficient to assess safety in the elements of that OI and ensure that safety will not be compromised and whether they might incur intolerably high costs.

An integrated ball projection technology for the study of dynamic interceptive actions.

PubMed

Stone, J A; Panchuk, D; Davids, K; North, J S; Fairweather, I; Maynard, I W

2014-12-01

Dynamic interceptive actions, such as catching or hitting a ball, are important task vehicles for investigating the complex relationship between cognition, perception, and action in performance environments. Representative experimental designs have become more important recently, highlighting the need for research methods to ensure that the coupling of information and movement is faithfully maintained. However, retaining representative design while ensuring systematic control of experimental variables is challenging, due to the traditional tendency to employ methods that typically involve use of reductionist motor responses such as buttonpressing or micromovements. Here, we outline the methodology behind a custom-built, integrated ball projection technology that allows images of advanced visual information to be synchronized with ball projection. This integrated technology supports the controlled presentation of visual information to participants while they perform dynamic interceptive actions. We discuss theoretical ideas behind the integration of hardware and software, along with practical issues resolved in technological design, and emphasize how the system can be integrated with emerging developments such as mixed reality environments. We conclude by considering future developments and applications of the integrated projection technology for research in human movement behaviors.

Balance in scientific impact assessment: the EGU Awards Committe experience

NASA Astrophysics Data System (ADS)

Montanari, Alberto

2016-04-01

Evaluation of scientific impact is becoming an essential step all over the world for assigning academic positions, funding and recognition. Impact is generally assessed by means of objective bibliometric indicators which are frequently integrated with a subjective evaluation by one or more individuals. An essential requirement of impact assessment is to ensure balance across several potential discriminating factors, including gender, ethnics, culture, scientific field and many others. Scientific associations need to ensure balance in any step of their activity and in particular when electing their representatives, evaluating scientific contributions, reviewing papers and assigning awards. While ensuring balance is a strict necessity, how to get to target is still a matter of vivid debates. In fact, the context of science is very different with respect to the general context of society and the need for scientific associations to maintain confidentiality in their evaluation procedures makes the application of transparent procedures more complicated. This talk aims to present the experience and the efforts of the European Geosciences Union to ensure balance, with a particular focus on gender balance. Data and statistics will be presented in the attempt to provide constructive indications to get to the target of giving equal opportunities to researchers across gender, continents and ethnic groups. Science is a unifying discipline and balance will be vital to ensure that humans and our planet co-evolve sustainably.

cual-id: Globally Unique, Correctable, and Human-Friendly Sample Identifiers for Comparative Omics Studies.

PubMed

Chase, John H; Bolyen, Evan; Rideout, Jai Ram; Caporaso, J Gregory

2016-01-01

The number of samples in high-throughput comparative "omics" studies is increasing rapidly due to declining experimental costs. To keep sample data and metadata manageable and to ensure the integrity of scientific results as the scale of these projects continues to increase, it is essential that we transition to better-designed sample identifiers. Ideally, sample identifiers should be globally unique across projects, project teams, and institutions; short (to facilitate manual transcription); correctable with respect to common types of transcription errors; opaque, meaning that they do not contain information about the samples; and compatible with existing standards. We present cual-id, a lightweight command line tool that creates, or mints, sample identifiers that meet these criteria without reliance on centralized infrastructure. cual-id allows users to assign universally unique identifiers, or UUIDs, that are globally unique to their samples. UUIDs are too long to be conveniently written on sampling materials, such as swabs or microcentrifuge tubes, however, so cual-id additionally generates human-friendly 4- to 12-character identifiers that map to their UUIDs and are unique within a project. By convention, we use "cual-id" to refer to the software, "CualID" to refer to the short, human-friendly identifiers, and "UUID" to refer to the globally unique identifiers. CualIDs are used by humans when they manually write or enter identifiers, while the longer UUIDs are used by computers to unambiguously reference a sample. Finally, cual-id optionally generates printable label sticker sheets containing Code 128 bar codes and CualIDs for labeling of sample collection and processing materials. IMPORTANCE The adoption of identifiers that are globally unique, correctable, and easily handwritten or manually entered into a computer will be a major step forward for sample tracking in comparative omics studies. As the fields transition to more-centralized sample management, for example, across labs within an institution, across projects funded under a common program, or in systems designed to facilitate meta- and/or integrated analysis, sample identifiers generated with cual-id will not need to change; thus, costly and error-prone updating of data and metadata identifiers will be avoided. Further, using cual-id will ensure that transcription errors in sample identifiers do not require the discarding of otherwise-useful samples that may have been expensive to obtain. Finally, cual-id is simple to install and use and is free for all use. No centralized infrastructure is required to ensure global uniqueness, so it is feasible for any lab to get started using these identifiers within their existing infrastructure.

Impact of North Atlantic Treaty Organization Policies and Procedures on Combined Medical Operations: Food and Water Safety and Veterinary Support.

PubMed

Stevenson, Timothy H; Chevalier, Nicole A; Scher, Gregory R; Burke, Ronald L

2016-01-01

Effective multilateral military operations such as those conducted by the North Atlantic Treaty Organization (NATO) require close cooperation and standardization between member nations to ensure interoperability. Failure to standardize policies, procedures, and doctrine prior to the commencement of military operations will result in critical interoperability gaps, which jeopardize the health of NATO forces and mission success. To prevent these gaps from occurring, US forces must be actively involved with NATO standardization efforts such as the Committee of the Chiefs of Medical Services to ensure US interests are properly represented when NATO standards are developed and US doctrine and procedures will meet the established NATO requirements.

Sample Preparation of Nano-sized Inorganic Materials for Scanning Electron Microscopy or Transmission Electron Microscopy: Scientific Operating Procedure SOP-P-2

DTIC Science & Technology

2015-07-01

samples must be thoroughly degreased and dried to eliminate any outgassing from organic contamination and water. Samples can be cleaned ultrasonically ...in an ultrasonic bath for at least 10 minutes to ensure separation of particles (University of California, Riverside, n.d.). These solutions can...be further agitated in the ultrasonic bath to separate particles. It should be noted that excessive ultrasonic agitation can damage samples

300 Area TEDF NPDES Permit Compliance Monitoring Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Loll, C.M.

1994-10-13

This monitoring plan describes the activities and methods that will be employed at the 300 Area Treated Effluent Disposal Facility (TEDF) in order to ensure compliance with the National Discharge Elimination System (NPDES) permit. Included in this document are a brief description of the project, the specifics of the sampling effort, including the physical location and frequency of sampling, the support required for sampling, and the Quality Assurance (QA) protocols to be followed in the sampling procedures.

Estimated dietary intake and risk assessment of polychlorinated dibenzo-p-dioxins and dibenzofurans and dioxin-like polychlorinated biphenyls from fish consumption in the Korean general population.

PubMed

Shin, Eun-su; Kim, Jongchul; Choi, Sung-Deuk; Kang, Young-Woon; Chang, Yoon-Seok

2016-03-01

We analyzed 17 polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and 12 dioxin-like polychlorinated biphenyls (DL-PCBs) in samples from various fish species available at food markets in nine Korean cities. The estimated dietary intake of these chemicals was calculated from the raw concentrations of PCDD/Fs and DL-PCBs in each sample and from the food consumption of the Korean general population, and a comparison was made with the provisional tolerable monthly intake (PTMI). The average daily dietary exposure and the 95th percentile of intake of PCDD/Fs and DL-PCBs were 0.21 and 0.49 pg WHO05-toxic equivalents (TEQ) kg(-1) body weight d(-1) representing 5.27% and 12.26%, respectively, of the Korean tolerable daily intake (TDI). We applied the monthly fish consumption limits to the evaluation of improved risk assessment and concluded that unlimited consumption of most fish species does not contribute to the elevated cancer risk. This investigation was the first such large-scale study in Korea, and incorporated 37 species, including a species of whale, and 480 samples. The major aims of this study were to demonstrate the health risks associated with fish intake and to ensure food safety through total analysis of PCDD/Fs and DL-PCBs using gas chromatography/high-resolution mass spectrometry (GC-HRMS). Copyright © 2015 Elsevier Ltd. All rights reserved.

Prospective power calculations for the Four Lab study of a multigenerational reproductive/developmental toxicity rodent bioassay using a complex mixture of disinfection by-products in the low-response region.

PubMed

Dingus, Cheryl A; Teuschler, Linda K; Rice, Glenn E; Simmons, Jane Ellen; Narotsky, Michael G

2011-10-01

In complex mixture toxicology, there is growing emphasis on testing environmentally representative doses that improve the relevance of results for health risk assessment, but are typically much lower than those used in traditional toxicology studies. Traditional experimental designs with typical sample sizes may have insufficient statistical power to detect effects caused by environmentally relevant doses. Proper study design, with adequate statistical power, is critical to ensuring that experimental results are useful for environmental health risk assessment. Studies with environmentally realistic complex mixtures have practical constraints on sample concentration factor and sample volume as well as the number of animals that can be accommodated. This article describes methodology for calculation of statistical power for non-independent observations for a multigenerational rodent reproductive/developmental bioassay. The use of the methodology is illustrated using the U.S. EPA's Four Lab study in which rodents were exposed to chlorinated water concentrates containing complex mixtures of drinking water disinfection by-products. Possible experimental designs included two single-block designs and a two-block design. Considering the possible study designs and constraints, a design of two blocks of 100 females with a 40:60 ratio of control:treated animals and a significance level of 0.05 yielded maximum prospective power (~90%) to detect pup weight decreases, while providing the most power to detect increased prenatal loss.

Modeling SF-6D Hong Kong standard gamble health state preference data using a nonparametric Bayesian method.

PubMed

Kharroubi, Samer A; Brazier, John E; McGhee, Sarah

2013-01-01

This article reports on the findings from applying a recently described approach to modeling health state valuation data and the impact of the respondent characteristics on health state valuations. The approach applies a nonparametric model to estimate a Bayesian six-dimensional health state short form (derived from short-form 36 health survey) health state valuation algorithm. A sample of 197 states defined by the six-dimensional health state short form (derived from short-form 36 health survey)has been valued by a representative sample of the Hong Kong general population by using standard gamble. The article reports the application of the nonparametric model and compares it to the original model estimated by using a conventional parametric random effects model. The two models are compared theoretically and in terms of empirical performance. Advantages of the nonparametric model are that it can be used to predict scores in populations with different distributions of characteristics than observed in the survey sample and that it allows for the impact of respondent characteristics to vary by health state (while ensuring that full health passes through unity). The results suggest an important age effect with sex, having some effect, but the remaining covariates having no discernible effect. The nonparametric Bayesian model is argued to be more theoretically appropriate than previously used parametric models. Furthermore, it is more flexible to take into account the impact of covariates. Copyright © 2013, International Society for Pharmacoeconomics and Outcomes Research (ISPOR). Published by Elsevier Inc.

Prospective Power Calculations for the Four Lab Study of A Multigenerational Reproductive/Developmental Toxicity Rodent Bioassay Using A Complex Mixture of Disinfection By-Products in the Low-Response Region

PubMed Central

Dingus, Cheryl A.; Teuschler, Linda K.; Rice, Glenn E.; Simmons, Jane Ellen; Narotsky, Michael G.

2011-01-01

In complex mixture toxicology, there is growing emphasis on testing environmentally representative doses that improve the relevance of results for health risk assessment, but are typically much lower than those used in traditional toxicology studies. Traditional experimental designs with typical sample sizes may have insufficient statistical power to detect effects caused by environmentally relevant doses. Proper study design, with adequate statistical power, is critical to ensuring that experimental results are useful for environmental health risk assessment. Studies with environmentally realistic complex mixtures have practical constraints on sample concentration factor and sample volume as well as the number of animals that can be accommodated. This article describes methodology for calculation of statistical power for non-independent observations for a multigenerational rodent reproductive/developmental bioassay. The use of the methodology is illustrated using the U.S. EPA’s Four Lab study in which rodents were exposed to chlorinated water concentrates containing complex mixtures of drinking water disinfection by-products. Possible experimental designs included two single-block designs and a two-block design. Considering the possible study designs and constraints, a design of two blocks of 100 females with a 40:60 ratio of control:treated animals and a significance level of 0.05 yielded maximum prospective power (~90%) to detect pup weight decreases, while providing the most power to detect increased prenatal loss. PMID:22073030

Evaluation of the BD Vacutainer Plus Urine C&S Preservative Tubes compared with nonpreservative urine samples stored at 4°C and room temperature.

PubMed

Eisinger, Stephen W; Schwartz, Matthew; Dam, Lisa; Riedel, Stefan

2013-09-01

The stability of urine specimens submitted for culture remains a challenge for many laboratories because of delays in specimen transport. We evaluated the usefulness of BD Vacutainer Plus Urine C&S Preservative Tube in ensuring specimen stability. Clinical urine specimens collected in sterile collection cups (n = 110) were plated onto sheep blood and MacConkey agar following standard laboratory procedures guidelines. Thereafter, specimens were divided into 3 storage conditions: nonpreservative, refrigerated; nonpreservative, room temperature (RT); BD Vacutainer Plus Urine C&S Preservative Tube, RT. For each sample type, additional cultures were set up at 2, 4, 24, and 48 hours. Initially, 18 specimens had no growth, 32 showed mixed skin flora, and 60 yielded at least 1 uropathogen. Increased colony counts of uropathogens were observed for nonpreserved urine samples stored at RT; these changes were statistically significant. Minor differences between refrigerated urine samples and BD Vacutainer Plus Urine C&S Preservative Tube samples were seen but were not statistically significant. The use of preservative-containing collection tubes is desirable to ensure specimen stability when prompt processing or refrigeration is not feasible.

40 CFR 63.997 - Performance test and compliance assessment requirements for control devices.

Code of Federal Regulations, 2010 CFR

2010-07-01

... as TOC minus methane and ethane according to the procedures specified. (i) Selection of sampling... regulated material or TOC, sampling sites shall be located as specified in paragraphs (e)(2)(i)(A)(1) and (e... shall ensure the measurement of total organic regulated material or TOC (minus methane and ethane...

40 CFR 65.158 - Performance test procedures for control devices.

Code of Federal Regulations, 2010 CFR

2010-07-01

... regulated material or as TOC (minus methane and ethane) according to the procedures specified. (1) Method 1... material or TOC, sampling sites shall be located at the inlet of the control device as specified in the... sampling sites shall ensure the measurement of total regulated material or TOC (minus methane and ethane...

STP Position Paper: Recommended Best Practices for Sampling, Processing and Analysis of the Peripheral Nervous System (Nerves and Somatic and Autonomic Ganglia) during Nonclinical Toxicity Studies

EPA Science Inventory

These Society of Toxicologic Pathology “best” practice recommendations should ensure consistent sampling, processing, and evaluation of the peripheral nervous system (PNS). For toxicity studies where neurotoxicity is not anticipated (Situation 1), PNS evaluation may be limited...

Passive Consent for Clinical Research in the Age of HIPAA

PubMed Central

Littenberg, Benjamin; MacLean, Charles D

2006-01-01

BACKGROUND Federal laws and regulations, including the Health Insurance Portability and Accountability Act (HIPAA) of 1996, intended primarily to protect individuals, have been described as significant barriers to the use of clinical registries and other population-based tools for health care research. Although these regulations allow for the waiver or alteration of usual consent procedures when the research meets certain specific criteria, waivers and alterations are rarely used in health care research. METHODS The Vermont Diabetes Information System is a multistate randomized trial of a quality improvement intervention that uses a novel alteration of informed consent to help ensure that the study sample is representative of the target population. Patients are notified by mail that they are eligible for the study and that they may opt out of the study, if they desire, by calling a toll-free number. RESULTS Seven thousand five hundred and fifty-eight patients were invited to participate. Two hundred and ten (2.8%) opted out. Three patients (0.04%) filed complaints, all of which were addressed satisfactorily. CONCLUSIONS Health Insurance Portability and Accountability Act and other federal regulations raise challenges to the use of clinical registries in research, but modifications to the consent process, including passive consent methods, are useful tools to overcome these challenges. It is possible to recruit a broad and representative population under current law while maintaining appropriate protections for research subjects. PMID:16637821

Data Specifications for the Landsat Data Continuity Mission

USGS Publications Warehouse

Irons, J.R.; Speciale, N.J.; Douglas, McCuistion J.; Masek, J.G.; Markham, B.L.; Storey, James C.; Lencioni, D.E.; Ryan, R.E.

2003-01-01

The National Aeronautics and Space Administration (NASA) plans to procure data from a privately-owned and commercially-operated remote sensing system for the next Landsat mission, the Landsat Data Continuity Mission (LDCM). Data requirements are documented in an LDCM Data Specification. The specifications require delivery of data covering 250 Landsat scenes on a daily basis. The data are to be acquired in a manner that affords seasonal coverage of the global land mass. Data are required for the heritage reflective Thematic Mapper (TM) spectral bands plus two new bands, a blue band for coastal zone observations and a short wave infrared band for cirrus cloud detection. The specifications do not require thermal data, representing a departure from the TM heritage. The specification also requires data providing a 30 m ground sample distance for each of the spectral bands with the exception of the new cirrus cloud band at 120 m. An absolute uncertainty of 5 percent or less is required for radiometrically corrected LDCM data and the commercial operator is required to deliver data that can be registered to a cartographic projection with an uncertainty of 65 m or less. Procuring data from a commercial operator represents a new approach for the 30-year-old Landsat Program. The LDCM Data Specification will ensure that the procured data provides continuity of the Landsat data stream and advances the mission.

High Contrast Imaging of Exoplanets and Exoplanetary Systems with JWST

NASA Astrophysics Data System (ADS)

Hinkley, Sasha; Skemer, Andrew; Biller, Beth; Baraffe, I.; Bonnefoy, M.; Bowler, B.; Carter, A.; Chen, C.; Choquet, E.; Currie, T.; Danielski, C.; Fortney, J.; Grady, C.; Greenbaum, A.; Hines, D.; Janson, M.; Kalas, P.; Kennedy, G.; Kraus, A.; Lagrange, A.; Liu, M.; Marley, M.; Marois, C.; Matthews, B.; Mawet, D.; Metchev, S.; Meyer, M.; Millar-Blanchaer, M.; Perrin, M.; Pueyo, L.; Quanz, S.; Rameau, J.; Rodigas, T.; Sallum, S.; Sargent, B.; Schlieder, J.; Schneider, G.; Stapelfeldt, K.; Tremblin, P.; Vigan, A.; Ygouf, M.

2017-11-01

JWST will transform our ability to characterize directly imaged planets and circumstellar debris disks, including the first spectroscopic characterization of directly imaged exoplanets at wavelengths beyond 5 microns, providing a powerful diagnostic of cloud particle properties, atmospheric structure, and composition. To lay the groundwork for these science goals, we propose a 39-hour ERS program to rapidly establish optimal strategies for JWST high contrast imaging. We will acquire: a) coronagraphic imaging of a newly discovered exoplanet companion, and a well-studied circumstellar debris disk with NIRCam & MIRI; b) spectroscopy of a wide separation planetary mass companion with NIRSPEC & MIRI; and c) deep aperture masking interferometry with NIRISS. Our primary goals are to: 1) generate representative datasets in modes to be commonly used by the exoplanet and disk imaging communities; 2) deliver science enabling products to empower a broad user base to develop successful future investigations; and 3) carry out breakthrough science by characterizing exoplanets for the first time over their full spectral range from 2-28 microns, and debris disk spectrophotometry out to 15 microns sampling the 3 micron water ice feature. Our team represents the majority of the community dedicated to exoplanet and disk imaging and has decades of experience with high contrast imaging algorithms and pipelines. We have developed a collaboration management plan and several organized working groups to ensure we can rapidly and effectively deliver high quality Science Enabling Products to the community.

A conceptual framework for the collection of food products in a Total Diet Study.

PubMed

Turrini, Aida; Lombardi-Boccia, Ginevra; Aureli, Federica; Cubadda, Francesco; D'Addezio, Laura; D'Amato, Marilena; D'Evoli, Laura; Darnerud, PerOla; Devlin, Niamh; Dias, Maria Graça; Jurković, Marina; Kelleher, Cecily; Le Donne, Cinzia; López Esteban, Maite; Lucarini, Massimo; Martinez Burgos, Maria Alba; Martínez-Victoria, Emilio; McNulty, Breige; Mistura, Lorenza; Nugent, Anne; Oktay Basegmez, Hatice Imge; Oliveira, Luisa; Ozer, Hayrettin; Perelló, Gemma; Pite, Marina; Presser, Karl; Sokolić, Darja; Vasco, Elsa; Volatier, Jean-Luc

2018-02-01

A total diet study (TDS) provides representative and realistic data for assessing the dietary intake of chemicals, such as contaminants and residues, and nutrients, at a population level. Reproducing the diet through collection of customarily consumed foods and their preparation as habitually eaten is crucial to ensure representativeness, i.e., all relevant foods are included and all potential dietary sources of the substances investigated are captured. Having this in mind, a conceptual framework for building a relevant food-shopping list was developed as a research task in the European Union's 7th Framework Program project, 'Total Diet Study Exposure' (TDS-Exposure), aimed at standardising methods for food sampling, analyses, exposure assessment calculations and modelling, priority foods, and selection of chemical contaminants. A stepwise approach following the knowledge translation (KT) model for concept analysis is proposed to set up a general protocol for the collection of food products in a TDS in terms of steps (characterisation of the food list, development of the food-shopping list, food products collection) and pillars (background documentation, procedures, and tools). A simple model for structuring the information in a way to support the implementation of the process, by presenting relevant datasets, forms to store inherent information, and folders to record the results is also proposed. Reproducibility of the process and possibility to exploit the gathered information are two main features of such a system for future applications.

Differentiating psychopathy from antisocial personality disorder: a triarchic model perspective.

PubMed

Venables, N C; Hall, J R; Patrick, C J

2014-04-01

The triarchic model of psychopathy characterizes the disorder in terms of three distinguishable phenotypic facets: disinhibition, meanness and boldness. The present study sought to (1) inform current debates regarding the role of boldness in the definition of psychopathy and (2) clarify boundaries between psychopathy and antisocial personality disorder (ASPD). This study evaluated the degree to which facets of the triarchic model are represented in the most widely used clinical inventory for psychopathy, the Psychopathy Checklist - Revised (PCL-R), in comparison with ASPD as defined by DSM-IV criteria. Adult male offenders from two distinct correctional settings (n = 157 and 169) were investigated to ensure replicability of findings across samples exhibiting high base rates of psychopathy and antisocial behavior. We found evidence for convergent and discriminant validity of the three triarchic facets in predicting symptomatic components of psychopathy as assessed by the PCL-R. Additionally, and crucially vis-à-vis current debates in the field, we found that boldness contributed incrementally (over and above disinhibition and meanness) to prediction of PCL-R psychopathy, in particular its interpersonal style component, but not ASPD. The three distinct facets of the triarchic model of psychopathy are represented clearly and distinctly in the PCL-R, with boldness through its interpersonal facet, but not in DSM-defined ASPD. Our findings suggest that boldness is central to diagnostic conceptions of psychopathy and distinguishes psychopathy from the more prevalent diagnosis of ASPD.

Structure and builtup of the Middle Bengal Fan at 8°N from multichannel seismic surveys and the IODP Expedition 354 drilling transect

NASA Astrophysics Data System (ADS)

Spiess, V.; Bergmann, F.; Schwenk, T.; Lantzsch, H.; Bahk, J. J.; Weber, M. E.; France-Lanord, C.; Klaus, A.

2016-12-01

IODP Expedition 354 to the Bengal Fan drilled a 320 km long e-W transect at 8°N with 7 drill sites, fully covering the uppermost 150-200 meters of fan deposits at distances of 50 km, originating from Himalayan mountain ranges and the Ganges-Brahmaputra river system. A major goal of this transect approach was to ensure a continuous record of turbiditic material delivered over the last appx. 1 million year, considering frequent longitudinal depocenter shifts of the active channel. By extensively utilizing the new half-APC coring technique, high quality and high recovery cores could be retrieved representing a wide range of grain sizes from hemipelagic deposits through clay rich turbidites to coarse silt and sandy units. Up to medium sand grain sizes were retrieved within the basal units of levees, which correspond to high-reflectivity units in high-resolution multichannel seismic profiles. Finely laminated sections with mm to cm-thick turbidites represents levee formations. At Site U1453 for example, core logging and downhole logging data confirm the representative sampling based on a very good match of several physical property data sets. An expanded section was cored at Site U1454, where the presumably currently active channel has built a levee, which likely represents major sediment supply within the last 30 kyr. A spatial grid of seismic and echosounder data in the vicinity of the active channel reveals a high spatial variability in sedimentation rates and distinct depocenter shifts in response to changes in channel geometry. Site U1452 has provided a full record a levee growth including interlevee sedimentation, sandy basal units characterized by a lobe formation, and a pronounced fining upward trend following the phase of channel erosion and levee builtup. From all sites, detailed comparisons of physical and sedimentological shipboard results with seismic data will be presented. Expedition 354 has provided a unique sample and data set to better understand fan deposition and channel-levee growth including silt and sand grain sizes. It will also provide valuable constraints on the volume, nature and composition of suspension flows contributing to fan growth in the Middle Bengal Fan.

Blood Culture Testing via a Mobile App That Uses a Mobile Phone Camera: A Feasibility Study

PubMed Central

Chong, Yong Pil; Jang, Seongsoo; Kim, Mi Na; Kim, Jeong Hoon; Kim, Woo Sung

2016-01-01

Background To evaluate patients with fever of unknown origin or those with suspected bacteremia, the precision of blood culture tests is critical. An inappropriate step in the test process or error in a parameter could lead to a false-positive result, which could then affect the direction of treatment in critical conditions. Mobile health apps can be used to resolve problems with blood culture tests, and such apps can hence ensure that point-of-care guidelines are followed and processes are monitored for blood culture tests. Objective In this pilot project, we aimed to investigate the feasibility of using a mobile blood culture app to manage blood culture test quality. We implemented the app at a university hospital in South Korea to assess the potential for its utilization in a clinical environment by reviewing the usage data among a small group of users and by assessing their feedback and the data related to blood culture sampling. Methods We used an iOS-based blood culture app that uses an embedded camera to scan the patient identification and sample number bar codes. A total of 4 medical interns working at 2 medical intensive care units (MICUs) participated in this project, which spanned 3 weeks. App usage and blood culture sampling parameters (including sampler, sampling site, sampling time, and sample volume) were analyzed. The compliance of sampling parameter entry was also measured. In addition, the participants’ opinions regarding patient safety, timeliness, efficiency, and usability were recorded. Results In total, 356/644 (55.3%) of all blood culture samples obtained at the MICUs were examined using the app, including 254/356 (71.3%) with blood collection volumes of 5-7 mL and 256/356 (71.9%) with blood collection from the peripheral veins. The sampling volume differed among the participants. Sampling parameters were completely entered in 354/356 cases (99.4%). All the participants agreed that the app ensured good patient safety, disagreed on its timeliness, and did not believe that it was efficient. Although the bar code scanning speed was acceptable, the Wi-Fi environment required improvement. Moreover, the participants requested feedback regarding their sampling quality. Conclusions Although this app could be used in the clinical setting, improvements in the app functions, environment network, and internal policy of blood culture testing are needed to ensure hospital-wide use. PMID:27784649

Blood Culture Testing via a Mobile App That Uses a Mobile Phone Camera: A Feasibility Study.

PubMed

Lee, Guna; Lee, Yura; Chong, Yong Pil; Jang, Seongsoo; Kim, Mi Na; Kim, Jeong Hoon; Kim, Woo Sung; Lee, Jae-Ho

2016-10-26

To evaluate patients with fever of unknown origin or those with suspected bacteremia, the precision of blood culture tests is critical. An inappropriate step in the test process or error in a parameter could lead to a false-positive result, which could then affect the direction of treatment in critical conditions. Mobile health apps can be used to resolve problems with blood culture tests, and such apps can hence ensure that point-of-care guidelines are followed and processes are monitored for blood culture tests. In this pilot project, we aimed to investigate the feasibility of using a mobile blood culture app to manage blood culture test quality. We implemented the app at a university hospital in South Korea to assess the potential for its utilization in a clinical environment by reviewing the usage data among a small group of users and by assessing their feedback and the data related to blood culture sampling. We used an iOS-based blood culture app that uses an embedded camera to scan the patient identification and sample number bar codes. A total of 4 medical interns working at 2 medical intensive care units (MICUs) participated in this project, which spanned 3 weeks. App usage and blood culture sampling parameters (including sampler, sampling site, sampling time, and sample volume) were analyzed. The compliance of sampling parameter entry was also measured. In addition, the participants' opinions regarding patient safety, timeliness, efficiency, and usability were recorded. In total, 356/644 (55.3%) of all blood culture samples obtained at the MICUs were examined using the app, including 254/356 (71.3%) with blood collection volumes of 5-7 mL and 256/356 (71.9%) with blood collection from the peripheral veins. The sampling volume differed among the participants. Sampling parameters were completely entered in 354/356 cases (99.4%). All the participants agreed that the app ensured good patient safety, disagreed on its timeliness, and did not believe that it was efficient. Although the bar code scanning speed was acceptable, the Wi-Fi environment required improvement. Moreover, the participants requested feedback regarding their sampling quality. Although this app could be used in the clinical setting, improvements in the app functions, environment network, and internal policy of blood culture testing are needed to ensure hospital-wide use.

Source technology as the foundation for modern infra-red counter measures (IRCM)

NASA Astrophysics Data System (ADS)

Grasso, Robert J.

2010-10-01

Protection of military aircraft from IR guided threats is paramount to ensure the survivability of aircrews, platforms, and to ensure mission success. At the foundation of all IRCM systems is the source; that component that provides the in-band radiant energy required for threat defeat. As such, source technology has evolved with IRCM technology to encompass the evolving systems architectures that encompass IRCM: 1) "Hot Brick" omni-directional sources; 2) arc lamps, and; 3) lasers. Lasers, as IRCM sources continue to evolve to meet the challenges of ever-evolving threats, superior techniques, economy of installation, and superior source technology. Lasers represent the single greatest advance in IRCM source technology and continue to evolve to meet ever more sophisticated threats. And have been used with great effect in all modern IRCM systems; evolving from frequency doubled CO2 lasers, to solid state lasers with OPOs, to semiconductor lasers including Quantum Cascade Lasers (QCLs); these last devices represent the latest advance in IRCM source technology offering all-band coverage, architectural simplicity, and economy of scale. While QCLs represent the latest advance in IRCM laser technology, fiber lasers show much promise in addressing multi-band operation as well as the ability to be coherently combined to achieve even greater output capability. Also, ultra-short pulse lasers are evolving to become practical for IRCM applications. Stay tuned ......

40 CFR Appendix A to Subpart E of... - Interim Transmission Electron Microscopy Analytical Methods-Mandatory and Nonmandatory-and...

Code of Federal Regulations, 2012 CFR

2012-07-01

... pore size less than or equal to 0.45 µm. 6. Place these filters in series with a 5.0 µm backup filter... for not more than 30 seconds and replacing it at the time of sampling before sampling is initiated at.... Ensure that the sampler is turned upright before interrupting the pump flow. 21. Check that all samples...

40 CFR Appendix A to Subpart E of... - Interim Transmission Electron Microscopy Analytical Methods-Mandatory and Nonmandatory-and...

Code of Federal Regulations, 2013 CFR

2013-07-01

... pore size less than or equal to 0.45 µm. 6. Place these filters in series with a 5.0 µm backup filter... for not more than 30 seconds and replacing it at the time of sampling before sampling is initiated at.... Ensure that the sampler is turned upright before interrupting the pump flow. 21. Check that all samples...

40 CFR Appendix A to Subpart E of... - Interim Transmission Electron Microscopy Analytical Methods-Mandatory and Nonmandatory-and...

Code of Federal Regulations, 2014 CFR

2014-07-01

... pore size less than or equal to 0.45 µm. 6. Place these filters in series with a 5.0 µm backup filter... for not more than 30 seconds and replacing it at the time of sampling before sampling is initiated at.... Ensure that the sampler is turned upright before interrupting the pump flow. 21. Check that all samples...

40 CFR 141.73 - Filtration.

Code of Federal Regulations, 2014 CFR

2014-07-01

... turbidity level of representative samples of a system's filtered water must be less than or equal to 0.5 NTU....74 (a)(1) and (c)(1). (2) The turbidity level of representative samples of a system's filtered water... filtration, the turbidity level of representative samples of a system's filtered water must be less than or...

Further statistics in dentistry. Part 4: Clinical trials 2.

PubMed

Petrie, A; Bulman, J S; Osborn, J F

2002-11-23

The principles which underlie a well-designed clinical trial were introduced in a previous paper. The trial should be controlled (to ensure that the appropriate comparisons are made), randomised (to avoid allocation bias) and, preferably, blinded (to obviate assessment bias). However, taken in isolation, these concepts will not necessarily ensure that meaningful conclusions can be drawn from the study. It is essential that the sample size is large enough to enable the effects of interest to be estimated precisely, and to detect any real treatment differences.

Online Recruitment: Feasibility, Cost, and Representativeness in a Study of Postpartum Women.

PubMed

Leach, Liana S; Butterworth, Peter; Poyser, Carmel; Batterham, Philip J; Farrer, Louise M

2017-03-08

Online recruitment is feasible, low-cost, and can provide high-quality epidemiological data. However, little is known about the feasibility of recruiting postpartum women online, or sample representativeness. The current study investigates the feasibility of recruiting a population of postpartum women online for health research and examines sample representativeness. Two samples of postpartum women were compared: those recruited online as participants in a brief survey of new mothers (n=1083) and those recruited face-to-face as part of a nationally representative study (n=579). Sociodemographic, general health, and mental health characteristics were compared between the two samples. Obtaining a sample of postpartum women online for health research was highly efficient and low-cost. The online sample over-represented those who were younger (aged 25-29 years), were in a de facto relationship, had higher levels of education, spoke only English at home, and were first-time mothers. Members of the online sample were significantly more likely to have poor self-rated health and poor mental health than the nationally representative sample. Health differences remained after adjusting for sociodemographic differences. Potential exists for feasible and low-cost e-epidemiological research with postpartum populations; however, researchers should consider the potential influence of sample nonrepresentativeness. ©Liana S Leach, Peter Butterworth, Carmel Poyser, Philip J Batterham, Louise M Farrer. Originally published in the Journal of Medical Internet Research (http://www.jmir.org), 08.03.2017.

Sampling Mars: Analytical requirements and work to do in advance

NASA Technical Reports Server (NTRS)

Koeberl, Christian

1988-01-01

Sending a mission to Mars to collect samples and return them to the Earth for analysis is without doubt one of the most exciting and important tasks for planetary science in the near future. Many scientifically important questions are associated with the knowledge of the composition and structure of Martian samples. Amongst the most exciting questions is the clarification of the SNC problem- to prove or disprove a possible Martian origin of these meteorites. Since SNC meteorites have been used to infer the chemistry of the planet Mars, and its evolution (including the accretion history), it would be important to know if the whole story is true. But before addressing possible scientific results, we have to deal with the analytical requirements, and with possible pre-return work. It is unlikely to expect that a possible Mars sample return mission will bring back anything close to the amount returned by the Apollo missions. It will be more like the amount returned by the Luna missions, or at least in that order of magnitude. This requires very careful sample selection, and very precise analytical techniques. These techniques should be able to use minimal sample sizes and on the other hand optimize the scientific output. The possibility to work with extremely small samples should not obstruct another problem: possible sampling errors. As we know from terrestrial geochemical studies, sampling procedures are quite complicated and elaborate to ensure avoiding sampling errors. The significance of analyzing a milligram or submilligram sized sample and putting that in relationship with the genesis of whole planetary crusts has to be viewed with care. This leaves a dilemma on one hand, to minimize the sample size as far as possible in order to have the possibility of returning as many different samples as possible, and on the other hand to take a sample large enough to be representative. Whole rock samples are very useful, but should not exceed the 20 to 50 g range, except in cases of extreme inhomogeneity, because for larger samples the information tends to become redundant. Soil samples should be in the 2 to 10 g range, permitting the splitting of the returned samples for studies in different laboratories with variety of techniques.

ACQUISITION OF REPRESENTATIVE GROUND WATER QUALITY SAMPLES FOR METALS

EPA Science Inventory

R.S. Kerr Environmental Research Laboratory (RSKERL) personnel have evaluated sampling procedures for the collection of representative, accurate, and reproducible ground water quality samples for metals for the past four years. Intensive sampling research at three different field...

Area of Concern (AOC) 314 Verification Survey at Former McClellan AFB, Sacramento, CA

DTIC Science & Technology

2015-03-31

also collected 22 soil samples from within AOC 314. Laboratory analysis revealed that the concentration of radium-226 (Ra-226) in 10 of the soil ...at least one sample that exceeded 2.0 pCi/g. The highest concentration of Ra-226 found in any of the soil samples was 25.8 pCi/g. Based on these...and ensure the potential health risk to future inhabitants is minimized. USAFSAM/OEC personnel also collected 22 soil samples from within AOC 314

Investigating the Effectiveness of Programs on Health Financing Based on Audit Procedures

PubMed Central

2016-01-01

Background: The present paper focuses on approaching the context and the actual manner of applying a method, known to be efficient and with a solid scientific background, on the institutional level of healthcare in Romania. Methods: The analyses and correlations developed by the author have taken into account the latest editions of the journals and publications of these institutions, such as statistical papers, standards, procedural guidebooks, reports. Most of the data (easily identifiable in the electronic environment) have been selected to enable the subsequent further investigation. Results: The payments made in 2012 for the audited NHP1–4, have increased by 82 282.66 thousand € (39.2%) as compared to 2010, and by 50578.22 thousand € (20.9%) as compared to 2011. The amounts paid for NHP1–4 have had an ascending trend starting with 2010, but, during 2010–2012, as the contribution of the Ministry of Health from the state budget has decreased from 152590.66 thousand € to 95328.22 thousand €, CNAS’s funding from the FNUASS resources has increased by 2.4 times. Conclusion: Following the analysis conducted on the NHP1–4, the findings based on representative samples are: (i) the specific legal standards related to the development of the NHP1–4, did not provide all the mechanisms that would ensure the certain procurement of medication and medical supplies as needed and did not ensure the efficient use of the allocated funds; (ii) clear discontinuities have been identified in the funding of NHP1–4, etc. PMID:27928534

Cavity-locked ring down spectroscopy

DOEpatents

Zare, Richard N.; Paldus, Barbara A.; Harb, Charles C.; Spence, Thomas

2000-01-01

Distinct locking and sampling light beams are used in a cavity ring-down spectroscopy (CRDS) system to perform multiple ring-down measurements while the laser and ring-down cavity are continuously locked. The sampling and locking light beams have different frequencies, to ensure that the sampling and locking light are decoupled within the cavity. Preferably, the ring-down cavity is ring-shaped, the sampling light is s-polarized, and the locking light is p-polarized. Transmitted sampling light is used for ring-down measurements, while reflected locking light is used for locking in a Pound-Drever scheme.

Blood rights: the body and information privacy.

PubMed

Alston, Bruce

2005-05-01

Genetic and other medical technology makes blood, human tissue and other bodily samples an immediate and accessible source of comprehensive personal and health information about individuals. Yet, unlike medical records, bodily samples are not subject to effective privacy protection or other regulation to ensure that individuals have rights to control the collection, use and transfer of such samples. This article examines the existing coverage of privacy legislation, arguments in favour of baseline protection for bodily samples as sources of information and possible approaches to new regulation protecting individual privacy rights in bodily samples.

Safer@home-Simulation and training: the study protocol of a qualitative action research design.

PubMed

Wiig, Siri; Guise, Veslemøy; Anderson, Janet; Storm, Marianne; Lunde Husebø, Anne Marie; Testad, Ingelin; Søyland, Elsa; Moltu, Kirsti L

2014-07-29

While it is predicted that telecare and other information and communication technology (ICT)-assisted services will have an increasingly important role in future healthcare services, their implementation in practice is complex. For implementation of telecare to be successful and ensure quality of care, sufficient training for staff (healthcare professionals) and service users (patients) is fundamental. Telecare training has been found to have positive effects on attitudes to, sustained use of, and outcomes associated with telecare. However, the potential contribution of training in the adoption, quality and safety of telecare services is an under-investigated research field. The overall aim of this study is to develop and evaluate simulation-based telecare training programmes to aid the use of videophone technology in elderly home care. Research-based training programmes will be designed for healthcare professionals, service users and next of kin, and the study will explore the impact of training on adoption, quality and safety of new telecare services. The study has a qualitative action research design. The research will be undertaken in close collaboration with a multidisciplinary team consisting of researchers and managers and clinical representatives from healthcare services in two Norwegian municipalities, alongside experts in clinical education and simulation, as well as service user (patient) representatives. The qualitative methods used involve focus group interviews, semistructured interviews, observation and document analysis. To ensure trustworthiness in the data analysis, we will apply member checks and analyst triangulation; in addition to providing contextual and sample description to allow for evaluation of transferability of our results to other contexts and groups. The study is approved by the Norwegian Social Science Data Services. The study is based on voluntary participation and informed written consent. Informants can withdraw at any point in time. The results will be disseminated at research conferences, peer review journals, one PhD thesis and through public presentations to people outside the scientific community. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

The IGSN Experience: Successes and Challenges of Implementing Persistent Identifiers for Samples

NASA Astrophysics Data System (ADS)

Lehnert, Kerstin; Arko, Robert

2016-04-01

Physical samples collected and studied in the Earth sciences represent both a research resource and a research product in the Earth Sciences. As such they need to be properly managed, curated, documented, and cited to ensure re-usability and utility for future science, reproducibility of the data generated by their study, and credit for funding agencies and researchers who invested substantial resources and intellectual effort into their collection and curation. Use of persistent and unique identifiers and deposition of metadata in a persistent registry are therefore as important for physical samples as they are for digital data. The International Geo Sample Number (IGSN) is a persistent, globally unique identifier. Its adoption by individual investigators, repository curators, publishers, and data managers is rapidly growing world-wide. This presentation will provide an analysis of the development and implementation path of the IGSN and relevant insights and experiences gained along its way. Development of the IGSN started in 2004 as part of a US NSF-funded project to establish a registry for sample metadata, the System for Earth Sample Registration (SESAR). The initial system provided a centralized solution for users to submit information about their samples and obtain IGSNs and bar codes. Challenges encountered during this initial phase related to defining the scope of the registry, granularity of registered objects, responsibilities of relevant actors, and workflows, and designing the registry's metadata schema, its user interfaces, and the identifier itself, including its syntax. The most challenging task though was to make the IGSN an integral part of personal and institutional sample management, digital management of sample-based data, and data publication on a global scale. Besides convincing individual researchers, curators, editors and publishers, as well as data managers in US and non-US academia, state and federal agencies, the PIs of the SESAR project needed to identify ways to organize, operate, and govern the global registry in the short and in the long-term. A major breakthrough was achieved at an international workshop in February 2011, at which participants designed a new distributed and scalable architecture for the IGSN with international governance by a membership organization modeled after the DataCite consortium. The founding of the international governing body and implementation organization for the IGSN, the IGSN e.V., took place at the AGU Fall Meeting 2011. Recent progress came at a workshop in September 2015, where stakeholders from both geoscience and life science disciplines drafted a standard IGSN metadata schema for describing samples, to complement the existing schema for registering samples. Consensus was achieved on an essential set of properties to describe a sample's origin and classification, creating a "birth certificate" for the sample. Further consensus was achieved in clarifying that an IGSN may represent exactly one physical sample, sampling feature, or collection of samples; and in aligning the IGSN schema with the existing Observations Data Model (ODM-2). The resulting schema was published online at schema.igsn.org and presented at the AGU Fall Meeting 2015.

Teaching LGBTQ-Themed Literature in English Language Arts Classrooms. Equity by Design

ERIC Educational Resources Information Center

Blackburn, Mollie; Miller, Mary Catherine

2017-01-01

The purpose of this Brief is to gain a better understanding of the barriers experienced by students who identify as lesbian, gay, bisexual, transgender, and queer/questioning (LGBTQ) and the need to ensure authentically represented within the curriculum, and to create a safe and inclusive learning environment. Data shows that students who identify…

Assessment of the Contribution of Regional Higher Education Systems to the Socio-Economic Development of the Russian Regions

ERIC Educational Resources Information Center

Leshukov, O. V.; Yevseyeva, D. G.; Gromov, A. D.; Platonova, D. P.

2017-01-01

This article analyzes how Russia's networks of higher education institutions contribute to their host regions in terms of the following three major facets: the economic development; the human capital development; and the innovative development. To ensure the analytical framework used derives relevant and representative findings given the nature of…

The Professional Pipeline for Educational Leadership. A White Paper Developed to Inform the Work of the National Policy Board for Educational Administration

ERIC Educational Resources Information Center

Hitt, Dallas Hambrick; Tucker, Pamela D.; Young, Michelle D.

2012-01-01

The professional pipeline represents a developmental perspective for fostering leadership capacity in schools and districts, from identification of potential talent during the recruitment phase to ensuring career-long learning through professional development. An intentional and mindful approach to supporting the development of educational leaders…

Expressing the sense of the House of Representatives that the United States Postal Service should take all appropriate measures to ensure the continuation of its 6-day mail delivery service.

THOMAS, 112th Congress

Rep. Graves, Sam [R-MO-6

2011-03-02

House - 03/18/2011 Referred to the Subcommittee on Federal Workforce, U.S. Postal Service, and Labor Policy. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Implementing Governance for a New Era: An Action Plan for Higher Education Trustees

ERIC Educational Resources Information Center

American Council of Trustees and Alumni, 2014

2014-01-01

"Implementing Governance for a New Era" is an action plan to assist college and university trustees in reforming higher education. Delivered to more than 16,000 college and university trustees in November 2014, the plan provides details on how trustees properly represent the public by ensuring students receive a quality education at a…

Aspiring and Practicing Leaders Addressing Issues of Diversity and Social Justice

ERIC Educational Resources Information Center

Bruner, Darlene Y.

2008-01-01

For the most part, faculty preparing teachers and leaders for tomorrow's schools would agree that educators, regardless of their role, need to be sensitive to the diverse cultures represented in our schools. Leaders need to be sensitive to and understanding of different cultures, while working to ensure the success of all students regardless of…

And never the twain shall meet? Integrating revenue cycle and supply chain functions.

PubMed

Matjucha, Karen A; Chung, Bianca

2008-09-01

Four initial steps to implementing a profit and loss management model are: Identify the supplies clinicians are using. Empower stakeholders to remove items that are not commonly used. Reduce factors driving wasted product. Review the chargemaster to ensure that supplies used in selected procedures are represented. Strategically set prices that optimize maximum allowable reimbursement.

The Sustainable Forestry Initiative of the American Forest & Paper Association

Treesearch

Chris Barneycastle

2001-01-01

The Sustainable Forestry Initiative (SFI)is a comprehensive program of forestry and conservation practices designed to ensure that future generations of Americans will have the same abundant forests that we enjoy today. The SFI was developed by the American Forest & Paper Association (AF&PA),the national trade group that represents forest and paper companies....

Missile Defense: European Phased Adaptive Approach Acquisitions Face Synchronization, Transparency, and Accountability Challenges

DTIC Science & Technology

2010-12-21

House of Representatives Subject: Missile Defense: European Phased Adaptive Approach Acquisitions Face Synchronization , Transparency, and...TITLE AND SUBTITLE Missile Defense: European Phased Adaptive Approach Acquisitions Face Synchronization , Transparency, and Accountability...However, we found that DOD has not fully implemented a management process that synchronizes EPAA acquisition activities and ensures transparency and

A Model for Postdoctoral Education That Promotes Minority and Majority Success in the Biomedical Sciences

ERIC Educational Resources Information Center

Eisen, Arri; Eaton, Douglas C.

2017-01-01

How does the United States maintain the highest-quality research and teaching in its professional science workforce and ensure that those in this workforce are effectively trained and representative of national demographics? In the pathway to science careers, the postdoctoral stage is formative, providing the experiences that define the…

An approach for verifying biogenic greenhouse gas emissions inventories with atmospheric CO2 concentration data

Treesearch

Stephen M Ogle; Kenneth Davis; Thomas Lauvaux; Andrew Schuh; Dan Cooley; Tristram O West; Linda S Heath; Natasha L Miles; Scott Richardson; F Jay Breidt; James E Smith; Jessica L McCarty; Kevin R Gurney; Pieter Tans; A Scott Denning

2015-01-01

Verifying national greenhouse gas (GHG) emissions inventories is a critical step to ensure that reported emissions data to the United Nations Framework Convention on Climate Change (UNFCCC) are accurate and representative of a country's contribution to GHG concentrations in the atmosphere. Furthermore, verifying biogenic fluxes provides a check on estimated...

National Educational Technology Trends: 2011. Transforming Education to Ensure All Students Are Successful in the 21st Century.

ERIC Educational Resources Information Center

Jones, Rachel; Fox, Christine; Levin, Douglas

2011-01-01

The State Educational Technology Directors Association (SETDA)--the principal association representing the technology leadership in all fifty states, the District of Columbia, the U.S. Virgin Islands, American Samoa, and the Bureau of Indian Affairs--presents its eighth annual report on select, national, educational technology activities. This…

The Training of Science Cadres of Sociohumanities Profile in Graduate School

ERIC Educational Resources Information Center

Balabanov, S. S.; Bednyi, B. I.; Mironos, A. A.

2008-01-01

This article analyzes problems relating to the effectiveness and the quality of the training of graduate students specializing in the area of the social sciences and the humanities. In order to ensure greater objectivity, account is taken of the opinions not only of humanities experts but also of representatives of the natural science disciplines.…

78 FR 79579 - Energy Conservation Program: Alternative Efficiency Determination Methods, Basic Model Definition...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-31

... Working Group agreed on a one- sided 5 percent tolerance when comparing the validation test results to the... one DOE representative. 78 FR 22431. The members of the Working Group were selected to ensure a broad... Issues 1. Pre-Approval The Working Group unanimously recommended that DOE not require pre- approval for...

Ensuring the Right to Minimally Adequate Habilitation: A Proposed Role for Human Rights Committees.

ERIC Educational Resources Information Center

Reese, R. Matthew

1984-01-01

The article suggests a role for Human Rights Committees in monitoring programs for mentally retarded persons in terms of whether the goals and objectives are adequate and whether the habilitation procedures represent a reasonable balance between intrusiveness and effectiveness and are being employed as indicated in the habilitation plan.…

Schools for the Twenty-First Century: School Design and Educational Transformation

ERIC Educational Resources Information Center

Leiringer, Roine; Cardellino, Paula

2011-01-01

The Building Schools for the Future programme has been established to ensure that English secondary schools are designed or redesigned to allow for educational transformation. The programme represents the biggest single UK government investment in school buildings for over 50 years. For this reason, it poses a major challenge to those involved in…

Shape Optimization for Navier-Stokes Equations with Algebraic Turbulence Model: Numerical Analysis and Computation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Haslinger, Jaroslav, E-mail: hasling@karlin.mff.cuni.cz; Stebel, Jan, E-mail: stebel@math.cas.cz

2011-04-15

We study the shape optimization problem for the paper machine headbox which distributes a mixture of water and wood fibers in the paper making process. The aim is to find a shape which a priori ensures the given velocity profile on the outlet part. The mathematical formulation leads to the optimal control problem in which the control variable is the shape of the domain representing the header, the state problem is represented by the generalized Navier-Stokes system with nontrivial boundary conditions. This paper deals with numerical aspects of the problem.

Avoiding treatment bias of REDD+ monitoring by sampling with partial replacement.

PubMed

Köhl, Michael; Scott, Charles T; Lister, Andrew J; Demon, Inez; Plugge, Daniel

2015-12-01

Implementing REDD+ renders the development of a measurement, reporting and verification (MRV) system necessary to monitor carbon stock changes. MRV systems generally apply a combination of remote sensing techniques and in-situ field assessments. In-situ assessments can be based on 1) permanent plots, which are assessed on all successive occasions, 2) temporary plots, which are assessed only once, and 3) a combination of both. The current study focuses on in-situ assessments and addresses the effect of treatment bias, which is introduced by managing permanent sampling plots differently than the surrounding forests. Temporary plots are not subject to treatment bias, but are associated with large sampling errors and low cost-efficiency. Sampling with partial replacement (SPR) utilizes both permanent and temporary plots. We apply a scenario analysis with different intensities of deforestation and forest degradation to show that SPR combines cost-efficiency with the handling of treatment bias. Without treatment bias permanent plots generally provide lower sampling errors for change estimates than SPR and temporary plots, but do not provide reliable estimates, if treatment bias occurs, SPR allows for change estimates that are comparable to those provided by permanent plots, offers the flexibility to adjust sample sizes in the course of time, and allows to compare data on permanent versus temporary plots for detecting treatment bias. Equivalence of biomass or carbon stock estimates between permanent and temporary plots serves as an indication for the absence of treatment bias while differences suggest that there is evidence for treatment bias. SPR is a flexible tool for estimating emission factors from successive measurements. It does not entirely depend on sample plots that are installed at the first occasion but allows for the adjustment of sample sizes and placement of new plots at any occasion. This ensures that in-situ samples provide representative estimates over time. SPR offers the possibility to increase sampling intensity in areas with high degradation intensities or to establish new plots in areas where permanent plots are lost due to deforestation. SPR is also an ideal approach to mitigate concerns about treatment bias.

Bullying during the Intermediate School Phase: A South African Study

ERIC Educational Resources Information Center

Greeff, P.; Grobler, A. A.

2008-01-01

Bullying in the intermediate school phase was studied, using the Revised Olweus Bully/Victim Questionnaire (R-OBVQ). The total sample comprised 360 grade 4 to 6 pupils from English-medium, single-sex schools in Bloemfontein, South Africa. To ensure a more homogeneous sample, the grade (grades 4 to 6) and race (black and white) of the participants…

NHEXAS PHASE I MARYLAND STUDY--STANDARD OPERATING PROCEDURE FOR CHAIN-OF-CUSTODY AND SAMPLE TRACKING (G04)

EPA Science Inventory

The purpose of the SOP is to establish the normal procedures for ensuring data chain-of-custody and data tracking. The chain-of-custody form included in this SOP is the standard form to be used for all data collected in the field. Keywords: samples; custody; records.

The Nat...

Planning Community-Based Assessments of HIV Educational Intervention Programs in Sub-Saharan Africa

ERIC Educational Resources Information Center

Kelcey, Ben; Shen, Zuchao

2017-01-01

A key consideration in planning studies of community-based HIV education programs is identifying a sample size large enough to ensure a reasonable probability of detecting program effects if they exist. Sufficient sample sizes for community- or group-based designs are proportional to the correlation or similarity of individuals within communities.…

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR EXTRACTION OF PESTICIDES FROM DERMAL WIPE SAMPLES (BCO-L-12.1)

EPA Science Inventory

The purpose of this SOP is to describe procedures for extracting and preparing a dermal wipe sample for GC/MS analysis of pesticides. This procedure was followed to ensure consistent data retrieval during the Arizona NHEXAS project and the "Border" study. Keywords: dermal wipe;...

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR COLLECTION OF SURFACE WIPE SAMPLES FOR PESTICIDES OR METALS (UA-F-8.1)

EPA Science Inventory

The purpose of this SOP is to describe the procedures for collecting surface wipe samples inside a home for analysis of either metals or pesticides. This procedure covers the preparation of the surface wipe material and field activities. This protocol was followed to ensure con...

Sampling and measurement protocols for long-term silvicultural studies on the Penobscot Experimental Forest

Treesearch

Justin D. Waskiewicz; Laura S. Kenefic; Nicole S. Rogers; Joshua J. Puhlick; John C. Brissette; Richard J. Dionne

2015-01-01

The U.S. Forest Service, Northern Research Station has been conducting research on the silviculture of northern conifers on the Penobscot Experimental Forest (PEF) in Maine since 1950. Formal study plans provide guidance and specifications for the experimental treatments, but documentation is also needed to ensure consistency in data collection and sampling protocols....

Environmental Response Laboratory Network (ERLN) Laboratory Requirements

EPA Pesticide Factsheets

The Environmental Response Laboratory Network requires its member labs follow specified quality systems, sample management, data reporting, and general, in order to ensure consistent analytical data of known and documented quality.

Parker THM Analyzer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hassan, Kazi; Siegal, Michael; Mowry, Curtis

This easy-to-operate, cost-effective, tabletop purge-and-trap gas chromatograph ensures safe drinking water and monitors disinfection by-product formation at water utilities in real-time without sample preparation or off-site analysis.

42 CFR 493.1451 - Standard: Technical supervisor responsibilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... testing samples; and (vi) Assessment of problem solving skills; and (9) Evaluating and documenting the... analysis and reporting of test results; (5) Resolving technical problems and ensuring that remedial actions...

Strengths and weaknesses of temporal stability analysis for monitoring and estimating grid-mean soil moisture in a high-intensity irrigated agricultural landscape

NASA Astrophysics Data System (ADS)

Ran, Youhua; Li, Xin; Jin, Rui; Kang, Jian; Cosh, Michael H.

2017-01-01

Monitoring and estimating grid-mean soil moisture is very important for assessing many hydrological, biological, and biogeochemical processes and for validating remotely sensed surface soil moisture products. Temporal stability analysis (TSA) is a valuable tool for identifying a small number of representative sampling points to estimate the grid-mean soil moisture content. This analysis was evaluated and improved using high-quality surface soil moisture data that were acquired by a wireless sensor network in a high-intensity irrigated agricultural landscape in an arid region of northwestern China. The performance of the TSA was limited in areas where the representative error was dominated by random events, such as irrigation events. This shortcoming can be effectively mitigated by using a stratified TSA (STSA) method, proposed in this paper. In addition, the following methods were proposed for rapidly and efficiently identifying representative sampling points when using TSA. (1) Instantaneous measurements can be used to identify representative sampling points to some extent; however, the error resulting from this method is significant when validating remotely sensed soil moisture products. Thus, additional representative sampling points should be considered to reduce this error. (2) The calibration period can be determined from the time span of the full range of the grid-mean soil moisture content during the monitoring period. (3) The representative error is sensitive to the number of calibration sampling points, especially when only a few representative sampling points are used. Multiple sampling points are recommended to reduce data loss and improve the likelihood of representativeness at two scales.

Effectiveness of vegetation-based biodiversity offset metrics as surrogates for ants.

PubMed

Hanford, Jayne K; Crowther, Mathew S; Hochuli, Dieter F

2017-02-01

Biodiversity offset schemes are globally popular policy tools for balancing the competing demands of conservation and development. Trading currencies for losses and gains in biodiversity value at development and credit sites are usually based on several vegetation attributes combined to yield a simple score (multimetric), but the score is rarely validated prior to implementation. Inaccurate biodiversity trading currencies are likely to accelerate global biodiversity loss through unrepresentative trades of losses and gains. We tested a model vegetation multimetric (i.e., vegetation structural and compositional attributes) typical of offset trading currencies to determine whether it represented measurable components of compositional and functional biodiversity. Study sites were located in remnant patches of a critically endangered ecological community in western Sydney, Australia, an area representative of global conflicts between conservation and expanding urban development. We sampled ant fauna composition with pitfall traps and enumerated removal by ants of native plant seeds from artificial seed containers (seed depots). Ants are an excellent model taxon because they are strongly associated with habitat complexity, respond rapidly to environmental change, and are functionally important at many trophic levels. The vegetation multimetric did not predict differences in ant community composition or seed removal, despite underlying assumptions that biodiversity trading currencies used in offset schemes represent all components of a site's biodiversity value. This suggests that vegetation multimetrics are inadequate surrogates for total biodiversity value. These findings highlight the urgent need to refine existing offsetting multimetrics to ensure they meet underlying assumptions of surrogacy. Despite the best intentions, offset schemes will never achieve their goal of no net loss of biodiversity values if trades are based on metrics unrepresentative of total biodiversity. © 2016 Society for Conservation Biology.

Logical Experimental Design and Execution in the Biomedical Sciences.

PubMed

Holder, Daniel J; Marino, Michael J

2017-03-17

Lack of reproducibility has been highlighted as a significant problem in biomedical research. The present unit is devoted to describing ways to help ensure that research findings can be replicated by others, with a focus on the design and execution of laboratory experiments. Essential components for this include clearly defining the question being asked, using available information or information from pilot studies to aid in the design the experiment, and choosing manipulations under a logical framework based on Mill's "methods of knowing" to build confidence in putative causal links. Final experimental design requires systematic attention to detail, including the choice of controls, sample selection, blinding to avoid bias, and the use of power analysis to determine the sample size. Execution of the experiment is done with care to ensure that the independent variables are controlled and the measurements of the dependent variables are accurate. While there are always differences among laboratories with respect to technical expertise, equipment, and suppliers, execution of the steps itemized in this unit will ensure well-designed and well-executed experiments to answer any question in biomedical research. © 2017 by John Wiley & Sons, Inc. Copyright © 2017 John Wiley & Sons, Inc.

Detection of Histoplasma capsulatum in Organic Fertilizers by Hc100 Nested Polymerase Chain Reaction and Its Correlation with the Physicochemical and Microbiological Characteristics of the Samples.

PubMed

Gómez, Luisa F; Torres, Isaura P; Jiménez-A, María Del Pilar; McEwen, Juan Gmo; de Bedout, Catalina; Peláez, Carlos A; Acevedo, José M; Taylor, María L; Arango, Myrtha

2018-05-01

Histoplasma capsulatum is the causative agent of histoplasmosis and this fungus inhabits soils rich in phosphorus and nitrogen that are enriched with bird and bat manure. The replacement of organic matter in agroecosystems is necessary in the tropics, and the use of organic fertilizers has increased. Cases and outbreaks due to the presence of the fungus in these components have been reported. The Instituto Colombiano Agropecuario resolution 150 of 2003 contains the parameters set by the Colombian Technical Standard (NTC 5167) on the physicochemical and microbiological features of fertilizers, but it does not regulate the search for H. capsulatum . The aim of this study was to demonstrate H. capsulatum presence in organic fertilizers by nested polymerase chain reaction (PCR). A total of 239 samples were collected: 201 (84.1%) corresponded to organic fertilizers, 30 (12.5%) to bird excrement, and 8 (3.4%) to cave soils. The Hc100 nested PCR had a detection limit of 0.1 pg/µL and a specificity of 100%. A total of 25 (10.5%) samples were positive and validated by sequencing. Seven of the positive samples represented locations where H. capsulatum was previously detected, suggesting the persistence of the fungus. No significant correlations were detected between the physicochemical and microbiological parameters with the presence of H. capsulatum by nested PCR, indicating the fungus existence in organic fertilizers that complied with the NTC 5167. The Hc100 nested PCR targeting H. capsulatum standardized in this work will improve the evaluation of organic fertilizers and ensure the prevention of outbreaks and cases due to manufacturing, marketing, and use of fertilizers contaminated with H. capsulatum .

Does the presence of caffeine in the marine environment represent an environmental risk? A regional and global study.

PubMed

Dafouz, Raquel; Cáceres, Neus; Rodríguez-Gil, José Luis; Mastroianni, Nicola; López de Alda, Miren; Barceló, Damià; de Miguel, Ángel Gil; Valcárcel, Yolanda

2018-02-15

Caffeine is an emerging contaminant considered to be an indicator of human contamination that has been widely detected in various aquatic systems, especially in continental waters. Nevertheless, the extent of its possible environmental impact is yet to be determined. This study determined the presence of caffeine, and evaluated the environmental hazard posed by this substance, in the "Rías Gallegas", a series of costal inlets in north-west Spain which are of great ecological value and in which fishing and bivalve farming, are a significant source of income. Caffeine was found to be present at concentrations higher than the limit of quantification (LOQ=3.07ngL -1 ) in 15 of the 23 samples analysed, with the highest seawater concentration being 857ngL -1 (the highest measured in seawater in Spain). Six out of 22 seawater samples resulted in a hazard quotient (HQ) from chronic exposure higher than 1 with the highest being 17.14, indicating a high probability of adverse effects in the aquatic environment. Environmental Exposure Distributions (EEDs) generated from a literature review of caffeine levels reported previously in four out of the five continents, showed that 28% of all seawater samples, and 69% of all estuary water samples where caffeine has ever been measured resulted in HQ>1 for chronic exposure. Further studies into the potential adverse effects that may arise from exposure to caffeine in aquatic systems are still required. Indeed, the need to gain a more in-depth understanding of the long-term ecotoxicological effects of caffeine is essential to ensure the quality of our health and environment. Copyright © 2017 Elsevier B.V. All rights reserved.

Evaluation of different pulverisation methods for RNA extraction in squash fruit: lyophilisation, cryogenic mill and mortar grinding.

PubMed

Román, Belén; González-Verdejo, Clara I; Peña, Francisco; Nadal, Salvador; Gómez, Pedro

2012-01-01

Quality and integrity of RNA are critical for transcription studies in plant molecular biology. In squash fruit and other high water content crops, the grinding of tissue with mortar and pestle in liquid nitrogen fails to produce a homogeneous and fine powered sample desirable to ensure a good penetration of the extraction reagent. To develop an improved pulverisation method to facilitate the homogenisation process of squash fruit tissue prior to RNA extraction without reducing quality and yield of the extracted RNA. Three methods of pulverisation, each followed by the same extraction protocol, were compared. The first approach consisted of the lyophilisation of the sample in order to remove the excess of water before grinding, the second one used a cryogenic mill and the control one a mortar grinding of frozen tissue. The quality of the isolated RNA was tested by carrying out a quantitative real time downstream amplification. In the three situations considered, mean values for A(260) /A(280) indicated minimal interference by proteins and RNA quality indicator (RQI) values were considered appropriate for quantitative real-time polymerase chain reaction (qRT-PCR) amplification. Successful qRT-PCR amplifications were obtained with cDNA isolated with the three protocols. Both apparatus can improve and facilitate the grinding step in the RNA extraction process in zucchini, resulting in isolated RNA of high quality and integrity as revealed by qRT-PCR downstream application. This is apparently the first time that a cryogenic mill has been used to prepare fruit samples for RNA extraction, thereby improving the sampling strategy because the fine powder obtained represents a homogeneous mix of the organ tissue. Copyright © 2012 John Wiley & Sons, Ltd.

Integration of multiple research disciplines on the International Space Station

NASA Technical Reports Server (NTRS)

Penley, N. J.; Uri, J.; Sivils, T.; Bartoe, J. D.

2000-01-01

The International Space Station will provide an extremely high-quality, long-duration microgravity environment for the conduct of research. In addition, the ISS offers a platform for performing observations of Earth and Space from a high-inclination orbit, outside of the Earth's atmosphere. This unique environment and observational capability offers the opportunity for advancement in a diverse set of research fields. Many of these disciplines do not relate to one another, and present widely differing approaches to study, as well as different resource and operational requirements. Significant challenges exist to ensure the highest quality research return for each investigation. Requirements from different investigations must be identified, clarified, integrated and communicated to ISS personnel in a consistent manner. Resources such as power, crew time, etc. must be apportioned to allow the conduct of each investigation. Decisions affecting research must be made at the strategic level as well as at a very detailed execution level. The timing of the decisions can range from years before an investigation to real-time operations. The international nature of the Space Station program adds to the complexity. Each participating country must be assured that their interests are represented during the entire planning and operations process. A process for making decisions regarding research planning, operations, and real-time replanning is discussed. This process ensures adequate representation of all research investigators. It provides a means for timely decisions, and it includes a means to ensure that all ISS International Partners have their programmatic interests represented. c 2000 Published by Elsevier Science Ltd. All rights reserved.

Joint use of over- and under-sampling techniques and cross-validation for the development and assessment of prediction models.

PubMed

Blagus, Rok; Lusa, Lara

2015-11-04

Prediction models are used in clinical research to develop rules that can be used to accurately predict the outcome of the patients based on some of their characteristics. They represent a valuable tool in the decision making process of clinicians and health policy makers, as they enable them to estimate the probability that patients have or will develop a disease, will respond to a treatment, or that their disease will recur. The interest devoted to prediction models in the biomedical community has been growing in the last few years. Often the data used to develop the prediction models are class-imbalanced as only few patients experience the event (and therefore belong to minority class). Prediction models developed using class-imbalanced data tend to achieve sub-optimal predictive accuracy in the minority class. This problem can be diminished by using sampling techniques aimed at balancing the class distribution. These techniques include under- and oversampling, where a fraction of the majority class samples are retained in the analysis or new samples from the minority class are generated. The correct assessment of how the prediction model is likely to perform on independent data is of crucial importance; in the absence of an independent data set, cross-validation is normally used. While the importance of correct cross-validation is well documented in the biomedical literature, the challenges posed by the joint use of sampling techniques and cross-validation have not been addressed. We show that care must be taken to ensure that cross-validation is performed correctly on sampled data, and that the risk of overestimating the predictive accuracy is greater when oversampling techniques are used. Examples based on the re-analysis of real datasets and simulation studies are provided. We identify some results from the biomedical literature where the incorrect cross-validation was performed, where we expect that the performance of oversampling techniques was heavily overestimated.