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Sample records for estudo da sua

  1. Construção de um catálogo de aglomerados abertos para estudo da dinâmica da estrutura espiral da Galáxia

    NASA Astrophysics Data System (ADS)

    Carlos, I. M.; Lépine, J. R. D.

    2003-08-01

    Os aglomerados abertos são objetos de grande valor para o estudo da dinâmica da Galáxia devido esses objetos terem uma faixa de idade relativamente ampla. O trabalho visa estudar a dinâmica da estrutura espiral da Galáxia principalmente através do uso desses aglomerados, uma vez que o estudo da cinemática desses objetos é fundamental para esse objetivo. Nosso grupo trabalha no sentido de construir uma base de dados de aglomerados abertos contendo coordenadas, distância, idade, movimentos próprios e velocidades radiais e já disponibiliza uma nova versão do catálogo de aglomerados abertos o qual é uma compilação de edições anteriores, principalmente Lynga (1987), Mermilliod (1995) e ESO-B (Lauberts 1982). Nossa amostra possui cerca de 1630 aglomerados, mas nem todos os parâmetros acima citados foram determinados em sua totalidade. Para determinarmos esses parâmetros, derivamos as cores intrínsecas das estrelas membro de cada aglomerado a partir de seus tipos espectrais (busca feita no SIMBAD) obtendo assim o excesso de cor individual. A distribuição dos excessos de cor foi então utilizada para derivarmos o avermelhamento médio para cada aglomerado. De maneira similar, os tipos espectrais foram usados para estimar as magnitudes absolutas, e com as magnitudes absolutas e aparentes determinamos a respectiva distribuição do módulo de distância e finalmente a distância. Para determinar as idades foram confeccionados os diagramas cor-magnitude das estrelas de cada aglomerado onde foram superpostas a Seqüência Principal de Idade Zero (ZAMS). Superpomos a ZAMS de Schmidt-Kaler e isócronas de composição solar. Essas isócronas foram usadas para determinação das idades dos aglomerados. Uma vez que não temos ainda resultados finais, apresentamos então alguns diagramas cor-magnitude os quais foram usados para determinação, principalmente, da distância e idade dos aglomerados.

  2. Um estudo espectrofotométrico da variável cataclísmica V3885 Sgr

    NASA Astrophysics Data System (ADS)

    Ribeiro, F. M. A.; Diaz, M. P.

    2003-08-01

    Variáveis Cataclísmicas são sistemas binários cerrados compostos de uma anã vermelha que transfere matéria para uma anã branca, em sistemas não magnéticos ocorre a formação de um disco de acresção em torno da anã branca. V3885 Sgr é uma variável cataclísmica classificada como sendo do tipo nova-like. É apresentado um estudo espectrofotométrico de V3885 Sgr de alta resolução temporal feito na região do visível. A região observada é centrada em Ha e abrange também a linha de HeI 6678. O primeiro resultado obtido neste estudo é a determinação do período orbital a partir de medidas da velocidade radial da linha de Ha como sendo 0,20716071(22) dias, resolvendo inconsistências quanto a esse valor na literatura e definindo uma efeméride a longo prazo para o sistema. Com este período e as medidas de velocidade radial do perfil de linha de Ha foi construído um diagrama de massas, através do qual restringimos as massas das componentes estelares do sistema e limitamos a inclinação orbital do sistema. Foram construídos diagramas de Greenstein para as linhas de Ha e HeI, onde os espectros médios em cada intervalo de fase são representados lado a lado em escala de cinza, indicando a existência de uma emissão intensa proveniente da parte posterior do disco. A partir da tomografia Doppler obtivemos perfis de emissividade radial para o disco tanto para a linha de Ha como para HeI. Os resultados obtidos são comparados com os de outros sistemas estudados com a mesma técnica. Serão apresentados também resultados da tomografia de flickering para o sistema.

  3. Estudo de não gaussianidade nas anisotropias da RCF medidas Wmap

    NASA Astrophysics Data System (ADS)

    Andrade, A. P. A.; Wuensche, C. A.; Ribeiro, A. L. B.

    2003-08-01

    A investigação do campo de flutuações da Radiação Cósmica de Fundo (RCF) pode oferecer um importante teste para os modelos cosmológicos que descrevem a origem e a evolução das flutuações primordiais. De um lado, apresenta-se o modelo inflacionário que prevê um espectro de flutuações adiabáticas distribuídas segundo uma gaussiana e, de outro, os modelos de defeitos topológicos (dentre outros) que descrevem um mecanismo para a geração de flutuações de isocurvatura que obedecem a uma distribuição não gaussiana. Este trabalho tem como objetivo caracterizar traços do modelo não gaussiano de campo misto (entre flutuações adiabáticas e de isocurvatura) nos mapas do Wilkinson Microwave Anisotropy Probe (WMAP). Simulações das anisotropias da RCF no contexto de mistura indicam traços marcantes na distribuição das flutuações de temperatura, mesmo quando consideradas pequenas contribuições do campo de isocurvatura (da ordem de 0.001). O efeito da mistura entre os campos resulta na transferência de potência de flutuações em escalas angulares intermediárias para flutuações em pequenas escalas angulares. Este efeito pode ser caracterizado pela relação entre as amplitudes dos primeiros picos acústicos no espectro de potência da RCF. Neste trabalho, investigamos a contribuição do campo de isocurvatura, no contexto de mistura, sobre as observações recentes da RCF realizadas pelo WMAP. As previsões do modelo de campo misto, uma vez confrontadas com as observações em pequenas escalas angulares, podem ajudar a revelar a natureza das flutuações primordiais.

  4. Botswana: Ntwetwe and Sua Pans

    Atmospheric Science Data Center

    2013-04-15

    ... of red band imagery in which the 45-degree aft camera data are displayed in blue, 45-degree forward as green, and vertical as red. ... coat the surface and turn it bright ("sua" means salt). The mining town of Sowa is located where the Sua Spit (a finger of grassland ...

  5. Educacao Intercultural e a Dificuldade de sua Pratica: Um Estudo da Imagem do Migrante e sua Familia em Livros Didaticos Alemaes. (Intercultural Education and the Difficulty of Its Practice: A Study of the Image of the Migrant and His Family in German Textbooks.)

    ERIC Educational Resources Information Center

    Weller, Wivian

    1995-01-01

    Provides a brief historical report on pedagogical efforts to improve the integration of migrants and their families into German society. Examines the way in which the migrants' social situation has been dealt with in textbooks, particularly in books on politics, history, geography, and occupational education. (PA)

  6. Modelagem do vento e da fotosfera de AG Carinae

    NASA Astrophysics Data System (ADS)

    Groh, J. H.; Damineli, A.

    2003-08-01

    A trajetória evolutiva das estrelas de alta massa depende fortemente de suas taxas de perda de massa. Apesar do rápido progresso no estudo destas estrelas, a taxa de perda de massa e outros parâmetros físicos básicos, como a temperatura superficial e a velocidade terminal do vento ainda não estão bem determinados. Isto ocorre devido à presença de ventos irregulares, rápidos e fortes ao redor destas estrelas, tornando a interpretação dos seus espectros uma tarefa difícil. Assim, a modelagem do vento e da fotosfera dessas estrelas está sendo cada vez mais usada para obter tais parâmetros a partir dos espectros. O aumento da taxa de perda de massa durante a fase LBV (Variáveis Luminosas Azuis), comparado com outros tipos de estrelas, tem sido atribuído a instabilidades do tipo S Doradus. Dispomos de uma base de dados espectroscópicos cobrindo 22 anos de observações de AG Carinae, incluindo um ciclo S Doradus completo, com espectros CCD em alta resolução na faixa óptica e infravermelha. Utilizamos o programa desenvolvido por Schmutz (1997) para uma análise preliminar desse ciclo, obtendo a taxa de perda de massa a partir da linha do Ha. Não existe uma correlação clara da taxa de perda de massa com mudanças da temperatura efetiva, do raio da estrela e do fluxo na banda V. A estrela atingiu seu mínimo fotométrico (raio mínimo) em 1990 e o máximo fotométrico (raio máximo) em 1995, enquanto que o fluxo máximo da linha do Ha ocorreu em 1996. Além disso a taxa de perda de massa não segue esse ciclo, contrariamente às idéias correntes. Para fazer um modelo mais realista estamos usando o programa CMFGEN (Hillier & Miller), que trata a fotosfera e o vento estelar de forma consistente, considerando a radiação fora do equilíbrio termodinâmico (NLTE) e com blanketting total de linhas. Simulamos o espectro de AG Carinae em duas épocas extremas do ciclo S Dor para testar os resultados obtidos com o modelo mais simplificado.

  7. Flutuações da temperatura eletrônica em NGC 2438 e NGC 2440

    NASA Astrophysics Data System (ADS)

    Krabbe, A. C.; Copetti, M. V. F.

    2003-08-01

    Tradicionalmente, as abundâncias em nebulosas planetárias e regiões HII têm sido obtidas de linhas excitadas colisionalmente, as quais são fortemente dependentes da temperatura eletrônica. Alternativamente, as abundâncias químicas podem ser determinadas de linhas de recombinação, as quais são quase independententes da temperatura eletrônica. Entretanto, consideráveis diferenças têm sido encontradas entre estes dois métodos. Estas discrepâncias têm sido atribuídas às flutuações espaciais da temperatura eletrônica. Entretanto, as magnitudes necessárias de flutuações de temperatura eletrônica são consideravelmente mais altas do que aquelas preditas pelos modelos padrões de fotoionização e os mecânismos físicos que poderiam explicar as grandes flutuações de temperatura são desconhecidas. Desta forma, o estudo de variações da temperatura eletrônica em regiões HII e nebulosas planetárias tem ganho um renovado interesse. Neste trabalho apresentamos os resultados preliminares de um estudo observacional sobre variações internas da temperatura eletrônica nas nebulosas planetárias NGC 2440 e NGC 2438. Analisamos dados espectrofotométricos de fenda longa, com alta razão sinal-ruído, na faixa de 3300-6800 Å, com o espectrógrafo Cassegrain Boller & Chivens acoplado ao telescópio de 1.52 m do European Southern Observatory (ESO). Temperaturas eletrônicas foram derivadas da razão [OIII](l4959+l5007)/l4363 em diferentes posições em cada nebulosa planetária. Flutuações da temperatura eletrônica foram detectadas e suas magnitudes foram estimadas em NGC 2440 e NGC 2438.

  8. DEZ ANOS DE EXPERIÊNCIA DO COMITÊ DE ÉTICA EM PESQUISA DA SECRETARIA DE SAÚDE DO DISTRITO FEDERAL, BRASIL

    PubMed Central

    Novaes, Maria Rita C. G.; Guilhem, Dirce; Lolas, Fernando

    2009-01-01

    O objetivo deste artigo é relatar a experiência do Comitê de Ética em Pesquisa da Secretaria de Estado de Saúde do Distrito Federal (CEP/SES/DF) Brasil, durante o período de 10 anos a partir de sua fundação. Trata-se de uma avaliação descritiva e documental, na modalidade estudo de caso, utilizando-se a totalidade de projetos protocolados no CEP/SES/DF (N° 052/08) nesse período. As pendências mais freqüentes dos projetos foram: termo de consentimento livre e esclarecido (30%), folha de rosto (25%), metodologia (20%), curriculum vitae (12%), planilha de orçamento (9%), outros (4%). O relato das atividades do CEP/SES/DF no período de 10 anos revelou, através de sua produtividade, a legitimidade do processo de análise ética dos protocolos visando à proteção dos participantes da pesquisa. PMID:19888441

  9. A Influência da Escola e da Mídia na Obtenção dos Conhecimentos Astronômicos

    NASA Astrophysics Data System (ADS)

    da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.

    2006-08-01

    O universo em que vivemos possui segredos jamais imaginados pelo homem, fruto da pequena proporção que ocupamos diante de um cosmos infinito. A astronomia funciona como um instrumento que sacia a sede humana em desvendar esses segredos, estudando as galáxias, quasares, pulsares além da existência de todos os elementos que preenchem o espaço cósmico, o vácuo, não mais vazio como se pensou em outros tempos. A mídia explora esse campo de forma mística e também científica. A difusão adequada e dentro dos padrões educacionais vigentes, de conceitos astronômicos, se faz necessária, devido sua presença nos conteúdos escolares, centros de ciência e na mídia, de modo geral. A reforma do Currículo Básico da Escola Pública da maioria dos Estados brasileiros introduziu conceitos astronômicos desde a pré-escola até o Ensino Médio. Num estudo realizado com 1.032 alunos do Ensino Médio de seis escolas estaduais, revelou-se através da aplicação de um questionário que abordava conheciment os básicos em astronomia, como estes foram adquiridos e qual era a infra-estrutura oferecida pela escola: para 66,2% dos entrevistados o professor já fez alguma apresentação a respeito de astronomia. O presente estudo revelou também que 44,5% dos alunos entrevistados adquiriram seus conhecimentos astronômicos na escola, 28,1% através da televisão e 27,4% afirmaram que seus conhecimentos foram obtidos através de outros tipos de mídia (internet, filmes e revistas). Embora o estudo revele claramente que há certo equilíbrio na forma em que os alunos obtiveram seus conhecimentos astronômicos, a mídia ainda explora e muitas vezes desperta o interesse pela astronomia com assuntos que não fazem parte de nosso cotidiano como, por exemplo, o homem na Lua, os projetos espaciais e a primeira viagem de um astronauta brasileiro no espaço. Porém, em certos casos, a mídia pode promover conhecimentos incompletos ou mesmo equivocados sobre os conceitos que envolvam

  10. Cooperative Autonomous Observation of Volcanic Environments with sUAS

    NASA Astrophysics Data System (ADS)

    Ravela, S.

    2015-12-01

    The Cooperative Autonomous Observing System Project (CAOS) at the MIT Earth Signals and Systems Group has developed methodology and systems for dynamically mapping coherent fluids such as plumes using small unmanned aircraft systems (sUAS). In the CAOS approach, two classes of sUAS, one remote the other in-situ, implement a dynamic data-driven mapping system by closing the loop between Modeling, Estimation, Sampling, Planning and Control (MESPAC). The continually gathered measurements are assimilated to produce maps/analyses which also guide the sUAS network to adaptively resample the environment. Rather than scan the volume in fixed Eulerian or Lagrangian flight plans, the adaptive nature of the sampling process enables objectives for efficiency and resilience to be incorporated. Modeling includes realtime prediction using two types of reduced models, one based on nowcasting remote observations of plume tracer using scale-cascaded alignment, and another based on dynamically-deformable EOF/POD developed for coherent structures. Ensemble-based Information-theoretic machine learning approaches are used for the highly non-linear/non-Gaussian state/parameter estimation, and for planning. Control of the sUAS is based on model reference control coupled with hierarchical PID. MESPAC is implemented in part on a SkyCandy platform, and implements an airborne mesh that provides instantaneous situational awareness and redundant communication to an operating fleet. SkyCandy is deployed on Itzamna Aero's I9X/W UAS with low-cost sensors, and is currently being used to study the Popocatepetl volcano. Results suggest that operational communities can deploy low-cost sUAS to systematically monitor whilst optimizing for efficiency/maximizing resilience. The CAOS methodology is applicable to many other environments where coherent structures are present in the background. More information can be found at caos.mit.edu.

  11. A correlação índice espectral vs. luminosidade em QSOs e suas implicações

    NASA Astrophysics Data System (ADS)

    Garcia-Rissmann, A.

    2003-08-01

    Estudos de variabilidade de núcleos ativos já demonstraram ser comum o fato de seu contínuo óptico/UV tornar-se mais "duro" à medida que a luminosidade aumenta. Essa tendência ocorre tanto de forma individual quanto global, e pode ter implicações importantes (1) para estudos fotométricos de variabilidade conduzidos numa banda fixa no referencial do observador, comparando objetos a diferentes redshifts, e (2) no cálculo da correção K, com consequente impacto na determinação de massas de buracos negros e bojos de galáxias hospedeiras (através da relação de Magorrian). Confirmo aqui as correlações positivas entre o índice espectral e a luminosidade óptica, utilizando dados espectroscópicos de 11 QSOs monitorados no Brasil e no Chile, durante ~2 anos. O estudo é complementado com parâmetros extraídos de espectros e de dados fotométricos públicos de quasares. Destaco ainda as diferenças observadas em tais correlações para objetos do tipo radio-loud e radio-quiet. Este projeto é financiado pelo I. Milênio/CNPq.

  12. In Aspergillus nidulans the Suppressors suaA and suaC Code for Release Factors eRF1 and eRF3 and suaD Codes for a Glutamine tRNA

    PubMed Central

    Liu, Wen; Mellado, Laura; Espeso, Eduardo A.; Sealy-Lewis, Heather M.

    2014-01-01

    In Aspergillus nidulans, after extensive mutagenesis, a collection of mutants was obtained and four suppressor loci were identified genetically that could suppress mutations in putative chain termination mutations in different genes. Suppressor mutations in suaB and suaD have a similar restricted spectrum of suppression and suaB111 was previously shown to be an alteration in the anticodon of a gln tRNA. We have shown that like suaB, a suaD suppressor has a mutation in the anticodon of another gln tRNA allowing suppression of UAG mutations. Mutations in suaA and suaC had a broad spectrum of suppression. Four suaA mutations result in alterations in the coding region of the eukaryotic release factor, eRF1, and another suaA mutation has a mutation in the upstream region of eRF1 that prevents splicing of the first intron within the 5′UTR. Epitope tagging of eRF1 in this mutant results in 20% of the level of eRF1 compared to the wild-type. Two mutations in suaC result in alterations in the eukaryotic release factor, eRF3. This is the first description in Aspergillus nidulans of an alteration in eRF3 leading to suppression of chain termination mutations. PMID:24727290

  13. Qual o Papel da Escola e da Mídia na Obtenção dos Conhecimentos Astronômicos?

    NASA Astrophysics Data System (ADS)

    da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.

    2007-08-01

    É amplamente reconhecido que as pessoas em geral têm grande fascínio e interesse pela Astronomia. Por outro lado, o conteúdo dessa área incluído no ensino formal está longe de ser abrangente e suficiente para suprir a demanda. Esse interesse permite aperfeiçoar a divulgação e o ensino não formal de Astronomia através da mídia aliando a adequação do conteúdo à expectativa das pessoas. A reforma do Currículo Básico da Escola Pública da maioria dos Estados brasileiros tem introduzido Astronomia desde a pré-escola até o Ensino Médio. Num estudo realizado com 1180 alunos do Ensino Médio de seis escolas estaduais, revelou-se através da aplicação de um formulário contendo questões que abordavam conhecimentos básicos em Astronomia, como foram adquiridos tais conhecimentos e à infra-estrutura da escola. Para 66,1% dos entrevistados o professor já fez alguma apresentação a respeito de Astronomia. O presente estudo revelou também que 54,4% dos alunos entrevistados adquiriram seus conhecimentos astronômicos na escola, 18,4% através da televisão e 27,1% afirmaram que seus conhecimentos foram obtidos através de outros tipos de mídia (internet, livros didáticos, filmes e revistas). Embora o estudo revele claramente que há certo equilíbrio no meio em que os alunos obtiveram seus conhecimentos astronômicos, a mídia busca não somente sua difusão, mas a disponibilização desse conhecimento de maneira correta, que às vezes, não é atingido, apresentando conceitos sem fundamentação e incorretos. Por outro lado, no dia-a-dia escolar, é comum encontrar professores que vêm para as aulas com concepções prévias, que podem diferir substancialmente das idéias a serem ensinadas, dificultando o aprendizado de futuros conceitos científicos. Uma avaliação criteriosa, avaliando as dificuldades dos professores em determinar o sentido correto dos conceitos e observando a metodologia que utilizam no ensino, poderá proporcionar uma melhor

  14. Providing Meaningful Learning for Students of the Sixth Grade of Middle School: a Study on the Moon Phases. (Breton Title: Propiciando Aprendizagem Significativa Para Alunos do Sexto Ano do Ensino Fundamental: um Estudo sobre as Fases da Lua.) Propiciando el Aprendizaje Significativo Para Alumnos del Sexto Nivel de la Educación General Básica: un Estudio sobre Las Fases de la Luna

    NASA Astrophysics Data System (ADS)

    Darroz, Luiz Marcelo; Samudio Pérez, Carlos Ariel; da Rosa, Cleci Werner; Heineck, Renato

    2012-07-01

    We relate in this article a didactic experience studying the moon phases with a group of middle school students of a private school of the municipality of Passo Fundo, RS. Based on David Ausubel's Meaningful Learning Theory, we have sought to develop a proposal following a didactic model which simulates the phases of the Moon, as based on the previous conceptions of the students. The signs of learning were evidenced by means of memory registries of the activity. From the obtained results we believe that the proposal achieved its goals, since the students were able to identify, differentiate and transfer the phenomenon of the moon phases to new contexts. Thus, it is concluded that a methodology focused on a meaningful content for the students is fundamental to the construction and genuine grasping of what is being learned. Neste artigo, relata-se uma experiência didática de estudo das fases da Lua com uma turma do 6° ano do Ensino Fundamental, de uma escola privada do município de Passo Fundo, RS. Tendo como fundamentação teórica a Teoria da Aprendizagem Significativa de David Ausubel, buscou-se desenvolver a proposta a partir de um modelo didático que simula as fases da Lua e com base nas concepções prévias dos estudantes. Os indícios da aprendizagem foram constatados através de registros de memórias da atividade. Pelos resultados apresentados, acredita-se que a proposta alcançou seus objetivos, uma vez que os estudantes conseguiram identificar, diferenciar e transferir o fenômeno das fases da Lua para novos contextos. Assim, conclui-se que uma metodologia com enfoque em um conteúdo significativo ao estudante é fundamental para a construção e compreensão genuína do que está sendo aprendido. En este artículo se relata una experiencia didáctica de estudio de las fases de la Luna con una clase de 6º año de la educación general básica de una escuela privada del municipio de Passo Fundo, RS. Teniendo como fundamentación teórica la Teor

  15. The Universe in a Box: Introduction to the Study of Astronomy in the Initial Formation of Physics Teachers. (Spanish Title: El Universo Representado en Una Caja: Introducción al Estudio de la Astronomía en la Formación Inicial de Profesores de Física.) O Universo Representado em Uma Caixa: Introdução ao Estudo da Astronomia NA Formação Inicial de Professores de Física

    NASA Astrophysics Data System (ADS)

    Longhini, Marcos Daniel

    2009-07-01

    This is a report of an activity of introduction to the study of Astronomy developed with a group of future Physics teachers at a Brazilian public university. Such activity had the goal of giving privileged emphasis to notions of spatiality, alternative conceptions of the participants and the process of interaction among peers, and consisted of the representation, in a three dimensional space, of the models of the universe that the participants had. The results, which were categorized as miscellaneous, geocentric, heliocentric and acentric models of the universe, were qualitatively analyzed. Analyses of the activity in the perspective of the participants are indicated and additional considerations are made regarding its use as a resource for teaching Astronomy and for teacher training. Este es el informe de una actividad para presentar un estudio introductorio de la Astronomía, desarrollado con una clase de futuros profesores de física en una universidad pública brasileña. Esta actividad tuvo como objetivo centrar las nociones de espacialidad, las concepciones alternativas de los participantes y el proceso de interacción entre pares, y consistió en la representación en un espacio tridimensional, de los modelos del universo que los participantes habían. Los resultados, que se clasificaron en universo miscelania, geocéntrico, heliocéntrico y acentrico, se analizaron cualitativamente. Son identificadas análisis de la actividad por los participantes, e hizo observaciones sobre su uso como recurso para la enseñanza de la astronomía y la formación de docentes. Trata-se do relato de uma atividade de introdução ao estudo da Astronomia, desenvolvida com uma turma de futuros professores de Física, em uma universidade pública brasileira. Tal atividade teve como meta privilegiar noções de espacialidade, as concepções alternativas dos participantes e o processo de interação entre pares e constou da representação, em um espaço tridimensional, dos

  16. sUAS and their application in observing geomorphological processes

    NASA Astrophysics Data System (ADS)

    Gallik, Jozef; Bolešová, Lenka

    2016-07-01

    Methodologies and procedures in processing gained data vary based on possibilities and needs of scientific projects. This paper should help to get a general overview in the choice of small unmanned aircraft systems (sUAS - commonly known as drones) for scientific purposes, namely remote sensing of geomorphologic processes such as soil degradation in high mountainous areas that are hard to access and have unfavourable weather conditions. All high mountain areas in European countries are legislatively protected, and so various permissions and observation of strict procedures are needed in order to not have a negative influence on the environment. Nowadays, several types of UAS exist that could effectively help us in such protection, as well as in full-fledged utilization when answering scientific questions about the alpine lake genesis. We demonstrate it here with selected examples of our photo documentation.

  17. Development and evaluation of a SUAS perching system

    NASA Astrophysics Data System (ADS)

    Reynolds, Ryan

    Perching has been proposed as a possible landing technique for Small Unmanned Aircraft Systems (SUAS). The current research study develops an onboard open loop perching system for a fixed-wing SUAS and examines the impact of initial flight speed and sensor placement on the perching dynamics. A catapult launcher and modified COTS aircraft were used for the experiments, while an ultrasonic sensor on the aircraft was used to detect the perching target. Thirty tests were conducted varying the initial launch speed and ultrasonic sensor placement to see if they affected the time the aircraft reaches its maximum pitch angle, since the maximum pitch angle is the optimum perching point for the aircraft. High-speed video was analyzed to obtain flight data, along with data from an onboard inertial measuring unit. The data were analyzed using a model 1, two-way ANOVA to determine if launch speed and sensor placement affect the optimum perching point where the aircraft reaches its maximum pitch angle during the maneuver. The results show the launch speed does affect the time at which the maximum pitch angle occurs, but sensor placement does not. This means a closed loop system will need to adjust its perching distance based on its initial velocity. The sensor placement not having any noticeable effect means the ultrasonic sensor can be placed on the nose or the wing of the aircraft as needed for the design. There was also no noticeable interaction between the two variables. Aerodynamic parameters such as lift, drag, and moment coefficients were derived from the dynamic equations of motion for use in numerical simulations and dynamic perching models.

  18. Unlocking the potential of small unmanned aircraft systems (sUAS) for Earth observation

    NASA Astrophysics Data System (ADS)

    Hugenholtz, C.; Riddell, K.; Barchyn, T. E.

    2012-12-01

    Small unmanned aircraft systems (sUAS, < 25 kg) are emerging as a viable alternative to conventional remote sensing platforms for Earth observation (EO). sUAS technology affords greater control, lower cost, and flexibility for scientists, and provides new opportunities to match the scale of sUAS data to the scale of the geophysical phenomenon under investigation. Although a mechanism is in place to make sUAS available to researchers and other non-military users through the US Federal Aviation Administration's Modernization and Reform Act of 2012 (FAAMRA), there are many regulatory hurdles before they are fully accepted and integrated into the National Airspace System. In this talk we will provide a brief overview of the regulatory landscape for sUAS, both in the USA and in Canada, where sUAS regulations are more flexible. We critically outline potential advantages and disadvantages of sUAS for EO applications under current and potential regulations. We find advantages: relatively low cost, potentially high temporal resolution, rapidly improving technology, and operational flexibility. We also find disadvantages: limited temporal and spatial extent, limited accuracy assessment and methodological development, and an immature regulatory landscape. From a case study we show an example of the accuracy of a photogrammetrically-derived digital terrain map (DTM) from sUAS imagery. We also compare the sUAS DTM to a LiDAR DTM. Our results suggest that sUAS-acquired imagery may provide a low-cost, rapid, and flexible alternative to airborne LiDAR. Overall, we are encouraged about the potential of sUAS for geophysical measurements; however, understanding and compliance with regulations is paramount to ensure that research is conducted legally and responsibly. Because UAS are new outside of military operations, we hope researchers will proceed carefully to ensure this great scientific opportunity remains a long term tool.

  19. Como os Alunos do Ensino Médio da Rede Estadual de São Paulo obtém Conhecimentos Astronômicos?

    NASA Astrophysics Data System (ADS)

    da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.

    2005-08-01

    Atualmente vivencia-se um mundo globalizado onde os computadores e a internet permitiram um acesso rápido e seguro a todo tipo de informação e conhecimento. O presente trabalho visa analisar a maneira pela qual alunos de segundo grau da rede estadual da cidade de São Paulo obtiveram, caso tenham, conhecimentos básicos de astronomia quanto aos fenômenos celestes que os rodeiam, tais como a sucessão dos dias e das estações do ano, além de questioná~los sobre fatos genéricos tais como: o que vem a ser o Sol, o Big Bang, o que ocasionou a extinção dos dinossauros. Para tanto foi elaborado um formulário constando de questões de múltipla escolha, o qual foi aplicado no primeiro colegial diurno da Escola Estadual Guilherme de Almeida. Num espaço amostral de 44 alunos constatou-se que 41% dos alunos adquiriram seus conhecimentos astronômicos na escola e 59% através da mídia em geral. Neste mesmo espaço amostral apenas 11% dos alunos usaram computadores na escola, 41% na residência, 5% no trabalho e 43% não utilizaram. O presente estudo revelou também que para 50% dos alunos o professor jamais utilizou um programa de computador a respeito de astronomia ou fez alguma apresentação sobre o tema. Embora em sua fase inicial este estudo revela claramente que a maioria dos alunos não obtém na escola seus conhecimentos astronômicos, estes provém de fontes não especificamente didático-pedagógicas tais como filmes e revistas populares que não raramente geram conhecimentos incompletos e em muitos casos inclusive falhos.

  20. A Reappraising of Cosmography: the Interface Between Astronomical and Geographic Studies. (Breton Title: Releitura do Conceito de Cosmografia: a Interface Entre os Estudos Astronômicos e Geográficos.) Una Relectura del Concepto de Cosmografía: la Interfase Entre los Estudios Astronómicos y Geográficos

    NASA Astrophysics Data System (ADS)

    Azevedo Sobreira, Paulo Henrique

    2012-12-01

    The concept of "Cosmography" is in disuse since the 80s of the last century, but the astronomical themes previously discussed in the school subjects of Geography and Cosmography remain in current textbooks. The use of term "Cosmography" was rescued in this research, and the study of its re-signification prompted the appearance of the term Geographic Cosmography. The Geographic Cosmography is a field of studies of the Geography, whose set of knowledge and skills is predominantly scholar. It studies the interface between terrestrial and celestial knowledge, and assigns a geographic significance to them. It examines human and natural relationships with Sidereal Space and its consequences for society and nature. O conceito de "Cosmografia" está em desuso desde os anos 80 do século XX, mas os temas astronômicos anteriormente abordados nas disciplinas escolares de Cosmografia e de Geografia permanecem nos atuais livros didáticos. O uso do termo "Cosmografia" foi resgatado nesta pesquisa e o estudo de sua ressignificação proporcionou o surgimento do termo Cosmografia Geográfica. A Cosmografia Geográfica é um campo de estudos da Geografia, cujo conjunto de conhecimentos e habilidades é predominantemente escolar. Estuda a interface entre os conhecimentos terrestres e os celestes e lhes atribui significância geográfica. Analisa as relações humanas e naturais com o Espaço Sideral e suas consequências para a sociedade e a natureza.

    Aunque el concepto de "Cosmografía" no se usa desde la década de los '80 del siglo pasado, los temas astronómicos que se enseñaban anteriormente en las asignaturas escolares de Cosmografía y de Geografía permanecen en los actuales libros didácticos. El uso del término "Cosmografía" fue rescatado en esta investigación y el estudio de su resignificación proporcionó el surgimiento del término Cosmografía Geográfica. La Cosmografía Geográfica es un campo de estudio de la Geografía, donde

  1. Study of the Local Horizon. (Spanish Title: Estudio del Horizonte Local.) Estudo do Horizonte Local

    NASA Astrophysics Data System (ADS)

    Ros, Rosa M.

    2009-12-01

    The study of the horizon is fundamental to easy the first observations of the students at any education center. A simple model, to be developed in each center, allows to easy the study and comprehension of the rudiments of astronomy. The constructed model is presented in turn as a simple equatorial clock, other models (horizontal and vertical) may be constructed starting from it. El estudio del horizonte es fundamental para poder facilitar las primeras observaciones de los alumnos en un centro educativo. Un simple modelo, que debe realizarse para cada centro, nos permite facilitar el estudio y la comprensión de los primeros rudimentos astronómicos. El modelo construido se presenta a su vez como un sencillo modelo de reloj ecuatorial y a partir de él se pueden construir otros modelos (horizontal y vertical). O estudo do horizonte é fundamental para facilitar as primeiras observações dos alunos num centro educativo. Um modelo simples, que deve ser feito para cada centro, permite facilitar o estudo e a compreensão dos primeiros rudimentos astronômicos. O modelo construído apresenta-se, por sua vez, como um modelo simples de relógio equatorial e a partir dele pode-se construir outros modelos (horizontal e vertical)

  2. The Teaching of Astronomy in Uruguay. (Spanish Title: La Enseñanza de la Astronomía en Uruguay.) O Ensino da Astronomia no Uruguai

    NASA Astrophysics Data System (ADS)

    Pintos Ganón, Reina; Fernández, Julio Angel

    2008-07-01

    We present in this work the development of astronomy in Uruguay since the colony times up to the present, stressing in particular those aspects related to the formal as well as informal teaching of astronomy. The first antecedents of astronomical studies in our country go back to the beginnings of the XVIIIth century related to the scientific expeditions from Europe coming to our shores. The teaching of astronomy was generalized at the high school level as soon as the end of the XIXth century, while itsintroduction at the university level happened much later, around the middle of the last century. We analyze the current situation of this subject, its problems, and some measures to strengthen its development. Se presenta en este trabajo el desarrollo de la astronomía en el Uruguay desde la época colonial hasta el presente, destacando en particular los aspectos vinculados a su enseñanza tanto formal como informal. Los primeros antecedentes de estudios astronómicos datan de la época colonial relacionados con las expediciones científicas del Viejo Mundo que llegaban a nuestras costas. La enseñanza de la Astronomía se generalizó en el ámbito de los estudios secundarios ya a fines del siglo XIX, mientras que en la Universidad su arribo fue mucho más tardío, a mediados del siglo pasado. Se analiza la situación actual de esta disciplina, sus problemas, y algunas medidas para fortalecer su desarrollo. Apresentamos, neste trabalho, o desenvolvimento da Astronomia no Uruguai desde a época colonial até o presente, destacando em particular os aspectos vinculados ao seu ensino tanto formal quanto informal. Os primeiros antecedentes de estudos astronômicos em nosso país datam da época colonial relacionados com as expedições científicas do Velho Mundo que chegavam às nossas costas. O ensino da Astronomia se generalizou no âmbito dos estudos secundários já no final do século XIX, enquanto que sua introdução na universidade foi muito mais tardia, em

  3. [Anogenital Warts in a Major Venereology Clinic: Centro de Saúde da Lapa - Lisbon, 2008 to 2014].

    PubMed

    Gameiro, Ana; Alves, João; Santo, Irene; Azevedo, Jacinta

    2016-02-01

    Introdução: O vírus do papiloma humano é responsável pela infeção sexualmente transmissível mais comum, podendo manifestar--se por um conjunto amplo de doenças, nomeadamente condilomas anogenitais, papilomatose laríngea recorrente, e neoplasias da região anogenital, colo do útero e orofaringe. Estima-se que os condilomas anogenitais afetem 1% da população sexualmente ativa, causados em cerca de 90% pelos genótipos 6 e 11. Material e Métodos: Identificámos os doentes com primeiro diagnóstico de condilomas anogenitais da consulta de doenças sexualmente transmissíveis do Centro de Saúde da Lapa, entre janeiro de 2008 e dezembro de 2014, e caracterizámos os doentes por sexo, orientação sexual, idade, localização das lesões, e número de parceiros nos últimos seis meses. Resultados: Foram identificados 902 indivíduos com primeiro diagnóstico de condilomas anogenitais. Observámos uma diminuição significativa de novos casos nas mulheres com < 19 anos (r = -0,848; p = 0,016), e uma diminuição sem significado estatístico nos homens com < 19 anos e nas mulheres entre os 20 - 24 anos. Discussão: Em outubro de 2008, a vacina quadrivalente (genótipos 6, 11, 16, 18) foi introduzida no plano nacional de vacinação de Portugal, abrangendo as adolescentes com 13 anos, com um catch-up para as de 17 anos. A diminuição de primeiros diagnósticos de condilomas anogenitais nas mulheres, deve-se provavelmente à sua vacinação antes de iniciarem a vida sexual. Conclusão: Este estudo reforça a import'ncia do programa nacional de vacinação para o vírus do papiloma humano.

  4. Overland Flow Analysis Using Time Series of Suas-Derived Elevation Models

    NASA Astrophysics Data System (ADS)

    Jeziorska, J.; Mitasova, H.; Petrasova, A.; Petras, V.; Divakaran, D.; Zajkowski, T.

    2016-06-01

    With the advent of the innovative techniques for generating high temporal and spatial resolution terrain models from Unmanned Aerial Systems (UAS) imagery, it has become possible to precisely map overland flow patterns. Furthermore, the process has become more affordable and efficient through the coupling of small UAS (sUAS) that are easily deployed with Structure from Motion (SfM) algorithms that can efficiently derive 3D data from RGB imagery captured with consumer grade cameras. We propose applying the robust overland flow algorithm based on the path sampling technique for mapping flow paths in the arable land on a small test site in Raleigh, North Carolina. By comparing a time series of five flights in 2015 with the results of a simulation based on the most recent lidar derived DEM (2013), we show that the sUAS based data is suitable for overland flow predictions and has several advantages over the lidar data. The sUAS based data captures preferential flow along tillage and more accurately represents gullies. Furthermore the simulated water flow patterns over the sUAS based terrain models are consistent throughout the year. When terrain models are reconstructed only from sUAS captured RGB imagery, however, water flow modeling is only appropriate in areas with sparse or no vegetation cover.

  5. Centro de Divulgação da Astronomia - o Observatório de São Carlos

    NASA Astrophysics Data System (ADS)

    Dias, W. S.; Hönel, J.; Líbero, V. L.; Anderlini, A.; de Andrade, D.; Scatena, E.; Lazo, M. J.; Trevisan, M.; de Castro, V.

    2005-07-01

    Apresentamos as principais características do Centro de Divulgação da Astronomia, que é constituído e um observatório cujo objetivo é a divulgação da Astronomia junto à comunidade, através de palestras e de observação direta dos astros. Descrevemos, neste trabalho, as atividades desenvolvidas desde sua fundação em 1986, e mostramos sua importância na região através de números anuais das atividades realizadas.

  6. On the Formation of a Study Group to the Realization of Workshops for Teachers: Astronomy in Basic Education in Umuarama-Pr (Spanish Title: De la Formación de un Grupo de Estudios a la Realización de los Talleres Para los Profesores: la Astronomía en la Educación Básica en Umuarama-Pr ) Da Formação de um Grupo de Estudos À Realização de Oficinas Para Professores: a Astronomia na Educação Básica em Umuarama-Pr

    NASA Astrophysics Data System (ADS)

    Belusso, Diane; Akira Sakai, Otávio

    2013-12-01

    execução da pesquisa e das oficinas promoveu o contato direto do grupo de estudos com a comunidade; os resultados serviram de diagnóstico do ensino-aprendizagem de astronomia, na educação básica, em Umuarama-PR.

  7. 76 FR 45300 - Notice of Issuance of Materials License SUA-1597 and Record of Decision for Uranerz Energy...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-28

    ... request for a materials license was previously noticed in the Federal Register on June 16, 2008 (73 FR... COMMISSION Notice of Issuance of Materials License SUA-1597 and Record of Decision for Uranerz Energy... of issuance of materials license SUA-1597. SUPPLEMENTARY INFORMATION: The Nuclear...

  8. The sua8 suppressors of Saccharomyces cerevisiae encode replacements of conserved residues within the largest subunit of RNA polymerase II and affect transcription start site selection similarly to sua7 (TFIIB) mutations.

    PubMed Central

    Berroteran, R W; Ware, D E; Hampsey, M

    1994-01-01

    Mutations in the Saccharomyces cerevisiae sua8 gene were found to be suppressors of an aberrant ATG translation initiation codon in the leader region of the cyc1 gene. Analysis of cyc1 transcripts from sua8 mutants revealed that suppression is a consequence of diminished transcription initiation at the normal start sites in favor of initiation at downstream sites, including a site between the aberrant and normal ATG start codons. This effect is not cyc1 gene specific since initiation at other genes, including ADH1, CYC7, and HIS4, was similarly affected, although initiation at HIS3 and SPT15 was unaffected. The SUA8 gene was cloned and partially sequenced, revealing identity to RPB1, which encodes the largest subunit of RNA polymerase II. The sua8 suppressors are the result of single amino acid replacements of highly conserved residues. Three replacements were found either within or immediately preceding homology block D, and a fourth was found adjacent to homology block H, indicating that these regions play a role in defining start sites in vivo. Nearly identical effects on start site selection were observed for sua7 suppressors, which encode altered forms of TFIIB. Synthetic lethality was associated with double sua7 sua8 suppressor mutations, and recessive sua7 mutants failed to fully complement recessive sua8 mutants in heterozygous diploids (nonallelic noncomplementation). These data indicate that the largest subunit of RNA polymerase II and TFIIB are important determinants of transcription start site selection in S. cerevisiae and suggest that this function might be conferred by interaction between these two proteins. Images PMID:8264591

  9. sUAS for Rapid Pre-Storm Coastal Characterization and Vulnerability Assessment

    NASA Astrophysics Data System (ADS)

    Brodie, K. L.; Slocum, R. K.; Spore, N.

    2015-12-01

    Open coast beaches and surf-zones are dynamic three-dimensional environments that can evolve rapidly on the time-scale of hours in response to changing environmental conditions. Up-to-date knowledge about the pre-storm morphology of the coast can be instrumental in making accurate predictions about coastal change and damage during large storms like Hurricanes and Nor'Easters. For example, alongshore variations in the shape of ephemeral sandbars along the coastline can focus wave energy, subjecting different stretches of coastline to significantly higher waves. Variations in beach slope and width can also alter wave runup, causing higher wave-induced water levels which can cause overwash or inlet breaching. Small Unmanned Aerial Systems (sUAS) offer a new capability to rapidly and inexpensively map vulnerable coastlines in advance of approaching storms. Here we present results from a prototype system that maps coastal topography and surf-zone morphology utilizing a multi-camera sensor. Structure-from-motion algorithms are used to generate topography and also constrain the trajectory of the sUAS. These data, in combination with mount boresight information, are used to rectify images from ocean-facing cameras. Images from all cameras are merged to generate a wide field of view allowing up to 5 minutes of continuous imagery time-series to be collected as the sUAS transits the coastline. Water imagery is then analyzed using wave-kinematics algorithms to provide information on surf-zone bathymetry. To assess this methodology, the absolute and relative accuracy of topographic data are evaluated in relation to simultaneously collected terrestrial lidar data. Ortho-rectification of water imagery is investigated using visible fixed targets installed in the surf-zone, and through comparison to stationary tower-based imagery. Future work will focus on evaluating how topographic and bathymetric data from this sUAS approach can be used to update forcing parameters in both

  10. Validation of Spaceborne Radar Surface Water Mapping with Optical sUAS Images

    NASA Astrophysics Data System (ADS)

    Li-Chee-Ming, J.; Murnaghan, K.; Sherman, D.; Poncos, V.; Brisco, B.; Armenakis, C.

    2015-08-01

    The Canada Centre for Remote Sensing (CCRS) has over 40 years of experience with airborne and spaceborne sensors and is now starting to use small Unmanned Aerial Systems (sUAS) to validate products from large coverage area sensors and create new methodologies for very high resolution products. Wetlands have several functions including water storage and retention which can reduce flooding and provide continuous flow for hydroelectric generation and irrigation for agriculture. Synthetic Aperture Radar is well suited as a tool for monitoring surface water by supplying acquisitions irrespective of cloud cover or time of day. Wetlands can be subdivided into three classes: open water, flooded vegetation and upland which can vary seasonally with time and water level changes. RADARSAT-2 data from the Wide-Ultra Fine, Spotlight and Fine Quad-Pol modes has been used to map the open water in the Peace-Athabasca Delta, Alberta using intensity thresholding. We also use spotlight modes for higher resolution and the fully polarimetric mode (FQ) for polarimetric decomposition. Validation of these products will be done using a low altitude flying sUAS to generate optical georeferenced images. This project provides methodologies which could be used for flood mapping as well as ecological monitoring.

  11. An Energy-Aware Trajectory Optimization Layer for sUAS

    NASA Astrophysics Data System (ADS)

    Silva, William A.

    The focus of this work is the implementation of an energy-aware trajectory optimization algorithm that enables small unmanned aircraft systems (sUAS) to operate in unknown, dynamic severe weather environments. The software is designed as a component of an Energy-Aware Dynamic Data Driven Application System (EA-DDDAS) for sUAS. This work addresses the challenges of integrating and executing an online trajectory optimization algorithm during mission operations in the field. Using simplified aircraft kinematics, the energy-aware algorithm enables extraction of kinetic energy from measured winds to optimize thrust use and endurance during flight. The optimization layer, based upon a nonlinear program formulation, extracts energy by exploiting strong wind velocity gradients in the wind field, a process known as dynamic soaring. The trajectory optimization layer extends the energy-aware path planner developed by Wenceslao Shaw-Cortez te{Shaw-cortez2013} to include additional mission configurations, simulations with a 6-DOF model, and validation of the system with flight testing in June 2015 in Lubbock, Texas. The trajectory optimization layer interfaces with several components within the EA-DDDAS to provide an sUAS with optimal flight trajectories in real-time during severe weather. As a result, execution timing, data transfer, and scalability are considered in the design of the software. Severe weather also poses a measure of unpredictability to the system with respect to communication between systems and available data resources during mission operations. A heuristic mission tree with different cost functions and constraints is implemented to provide a level of adaptability to the optimization layer. Simulations and flight experiments are performed to assess the efficacy of the trajectory optimization layer. The results are used to assess the feasibility of flying dynamic soaring trajectories with existing controllers as well as to verify the interconnections between

  12. CONHECIMENTO DA LEI GERAL DE SAÚDE – RESPEITO ÀS TRANSFUSÕES SANGUÍNEAS EM MÉDICOS E PACIENTES TESTEMUNHAS DE JEOVÁ DO HOSPITAL DR. DARÍO CONTRERAS DA REPÚBLICA DOMINICANA

    PubMed Central

    SANTANA, ELSA DÍAZ

    2010-01-01

    Este estudo avalia quanto o corpo médico do Hospital Dr. Darío Contreras de República Dominicana conhece, respeita, informa e aplica a Lei Geral de Saúde em relação aos direitos do paciente Testemunha de Jeová de negar-se a ser transfundido (respeito a sua autonomia); também se os Testemunhas de Jeová conhecem a Lei Geral de Saúde e até que ponto têm se beneficiado diante dessa proposição. O estudo revelou que nem médicos, nem Testemunhas de Jeová conhecem de fato essa lei. PMID:20689657

  13. University Students' Conceptions about the Moon Phases. (Spanish Title: Concepciones de Estudiantes Universitários sobre Las Fases de la Luna.) Concepções de Estudantes Universitários sobre as Fases da Lua

    NASA Astrophysics Data System (ADS)

    de Fátima Oliveira Saraiva, Maria; da Silveira, Fernando Lang; Steffani, Maria Helena

    2011-07-01

    teste notamos que a porcentagem de acertos a respeito de alguns conceitos teve um aumento significativo associado à reformulação da pergunta, ressaltando a importância de tomar cuidado para evitar respostas erradas geradas por perguntas pouco claras e não por ignorância do respondente sobre o assunto. Confirmamos os resultados de outros estudos de que as maiores dificuldades dos alunos sobre o tema Fases da Lua dizem respeito a relacionar a fase que a Lua apresenta com a sua posição no céu em determinada hora. Por outro lado, nossos resultados sugerem que, em geral, os estudantes de Física entendem melhor o fenômeno das fases lunares do que a média dos estudantes universitários.

  14. 76 FR 53500 - Notice of the Nuclear Regulatory Commission Issuance of Materials License SUA-1598 and Record of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Notice of the Nuclear Regulatory Commission Issuance of Materials License SUA-1598 and Record of Decision for Lost Creek ISR, LLC Lost Creek In-Situ Recovery Project AGENCY: U.S. Nuclear...

  15. 75 FR 62153 - Notice of the Nuclear Regulatory Commission Issuance of Materials License SUA-1596 for Uranium...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-07

    ... request for a materials license was previously noticed in the Federal Register on January 25, 2008 (73 FR... COMMISSION Notice of the Nuclear Regulatory Commission Issuance of Materials License SUA-1596 for Uranium One... Commission (NRC) has issued a license to Uranium One Americas, Inc. (Uranium One) for its Moore Ranch...

  16. Estudo comparativo entre estrelas centrais de nebulosas planetárias deficientes em hidrogênio

    NASA Astrophysics Data System (ADS)

    Marcolino, W. L. F.; de Araújo, F. X.

    2003-08-01

    Apresentamos neste trabalho o resultado de um estudo das principais características espectrais das estrelas centrais de nebulosas planetárias (ECNP) deficientes em hidrogênio. A origem e a evolução dessas estrelas ainda constitui um problema em aberto na evolução estelar. Geralmente esses objetos são divididos em [WCE], [WCL] e [WELS]. Os tipos [WCE] e [WCL] apresentam um espectro típico de uma estrela Wolf-Rayet carbonada de população I e as [WELS] apresentam linhas fracas de carbono e oxigênio em emissão. Existem evidências que apontam a seguinte sequência evolutiva : [WCL] = > [WCE] = > [WELS] = > PG 1159 (pré anã-branca). No entanto, tal cenário apresenta falhas como por exemplo a falta de ECNP entre os tipos [WCL] e [WCE]. Baseados em uma amostra de 24 objetos obtida no telescópio de 1.52m em La Silla, Chile (acordo ESO/ON), ao longo do ano 2000, apresentamos os resultados da comparação das larguras equivalentes de diversas linhas relevantes entre os tipos [WCL], [WCE] e [WELS]. Verificamos que nossos dados estão de acordo com a sequência evolutiva. Baseado nas linhas de C IV, conseguimos dividir pela primeira vez as [WELS] em dois grupos principais. Além disso, os dados reforçam a afirmação de que as [WCE] são as estrelas que possuem a maior temperatura entre as ECNP deficientes em hidrogênio. Discutimos ainda, a escassez de dados disponíveis na literatura e a necessidade da obtenção de parametros físicos para estes objetos.

  17. Genotyping and study of the pauA and sua genes of Streptococcus uberis isolates from bovine mastitis.

    PubMed

    Perrig, Melina S; Ambroggio, María B; Buzzola, Fernanda R; Marcipar, Iván S; Calvinho, Luis F; Veaute, Carolina M; Barbagelata, María Sol

    2015-01-01

    This study aimed to determine the clonal relationship among 137 Streptococcus uberis isolates from bovine milk with subclinical or clinical mastitis in Argentina and to assess the prevalence and conservation of pauA and sua genes. This information is critical for the rational design of a vaccine for the prevention of bovine mastitis caused by S. uberis. The isolates were typed by random amplified polymorphic DNA (RAPD) analysis and by pulsed-field gel electrophoresis (PFGE). The 137 isolates exhibited 61 different PFGE types and 25 distinct RAPD profiles. Simpson's diversity index was calculated both for PFGE (0.983) and for RAPD (0.941), showing a high discriminatory power in both techniques. The analysis of the relationship between pairs of isolates showed 92.6% concordance between both techniques indicating that any given pair of isolates distinguished by one method tended to be distinguished by the other. The prevalence of the sua and pauA genes was 97.8% (134/137) and 94.9% (130/137), respectively. Nucleotide and amino acid sequences of the sua and pauA genes from 20 S. uberis selected isolates, based on their PFGE and RAPD types and geographical origin, showed an identity between 95% and 100% with respect to all reference sequences registered in GenBank. These results demonstrate that, in spite of S. uberis clonal diversity, the sua and pauA genes are prevalent and highly conserved, showing their importance to be included in future vaccine studies to prevent S. uberis bovine mastitis.

  18. Respostas religiosas à aids no Brasil: impressões de pesquisa acerca da Pastoral de DST/Aids da Igreja Católica1

    PubMed Central

    Seffner, Fernando; Silva, Cristiane Gonçalves Meireles da; Maksud, Ívia; Garcia, Jonathan; Rios, Luís Felipe; Natividade, Marcelo; Borges, Priscila Rodrigues; Parker, Richard; Terto, Veriano

    2009-01-01

    O texto encontra-se estruturado em quatro partes. Na primeira delas, apresentamos um conjunto de considerações e informações acerca da situação da aids no Brasil, das relações entre religião, sexualidade, aids e estado laico, bem como uma descrição mais clara do Projeto Respostas Religiosas ao HIV/Aids no Brasil, do qual este texto apresenta algumas impressões de pesquisa preliminares. A seguir, dedicamos um item a apresentação da Pastoral de DST/Aids, sua história, estrutura e objetivos. No item seguinte problematizamos diversas questões em particular no âmbito das relações Estado e Igreja, relações entre agentes de pastoral e hierarquia da Igreja, e questões ligadas mais diretamente à sexualidade e aids, todas referenciadas ao trabalho da Pastoral de DST/Aids. Ao final, apresentamos a bibliografia e fontes consultadas. PMID:20442806

  19. Os Portugueses No Sudoeste Da Nova Inglaterra E A Sua Literatura Infantil (The Portuguese in Southeast New England and Their Children's Literature).

    ERIC Educational Resources Information Center

    Clinkscales, Rosalie Duggan

    The four sections of this paper deal with Portuguese immigrants in southeast New England and with Portuguese-language children's literature. The first section contains a description of Portuguese immigration to the United States from 1511 to the present and notes changes in immigration laws. The second section presents descriptions of the…

  20. Rotação do jato em DG tau próximo à região de sua formação: análise comparativa das velocidades radiais simuladas e observadas

    NASA Astrophysics Data System (ADS)

    Cerqueira, A. H.; de Gouveia dal Pino, E. M.

    2003-08-01

    Os modelos magneto-centrífugos utilizados para explicar a formação dos jatos Herbig-Haro assumem a presença de um disco de acresção em rotação kepleriana na base de lançamento do jato. Neste cenário, o jato transmite a informação da rotação do disco para regiões distantes da fonte central, além da superfície de Alfvén, na região de colimação inicial do jato. Recentemente, Bacciotti et al. (2002, ApJ, 537, L49) obtiveram pela primeira vez uma evidência observacional de rotação em um jato HH, o jato em DG Tau, em regiões próximas da fonte central, compatível (qualitativa e quantitativamente) com o esperado a partir dos modelos magneto-centrífugos para a produção e colimação inicial de jatos HH. No presente trabalho, apresentamos mapas de velocidade radial, obtidos através de simulações numéricas tri-dimensionais SPH, para um jato com características semelhantes ao jato em DG Tau, objetivando uma comparação com os mapas de velocidade radiais obtidos por Bacciotti et al.. Nossos resultados, embora preliminares, indicam que a inclusão de efeitos como a precessão, evidenciada em DG Tau (Dougados et al. 2000, A&A, 357, L61) devem ser levadas em consideração na análise da presença de rotação não só em DG Tau mas em qualquer sistema, com o uso das velocidades radias observadas. A ausência de um grau elevado de simetria axial (quebrada, por exemplo, pela precessão do eixo do jato; ou pela presença de uma superfície interna de trabalho, ou seja, um bow shock interno), implica também em uma maior complexidade nos mapas, com conseqüências relevantes para suas interpretações.

  1. A Utilização da Astronomia como Tema Interdisciplinar e Aplicações de Objetos de Aprendizagem

    NASA Astrophysics Data System (ADS)

    da Silva, L. A.; Voelzke, M. R.

    2008-09-01

    Este trabalho visa analisar a possibilidade de relacionar conteúdos aplicados no ensino fundamental e médio de forma interdisciplinar por intermédio da astronomia, com a intervenção de objetos de aprendizagem que possam integrar as disciplinas e a utilização de recursos tecnológicos. Em uma pesquisa prévia com 20 professores de uma escola estadual situada na cidade de Guarulhos foi observado que apenas 25% dos professores utilizam algum recurso tecnológico para o desenvolvimento de conteúdos pertinentes à sua disciplina, tais como sites e softwares educativos, sendo que a maioria absoluta continua ensinando apenas com livros didáticos. A maior parte dos professores apresenta dificuldades em trabalhar sua disciplina de forma interdisciplinar, ou seja, 75% dos professores preferem aplicar os conteúdos seguindo uma hierarquia linear de tópicos, evitando a discussão de temas que de alguma forma estão relacionados. A astronomia pode vir à fascinar o ser humano e despertar sua curiosidade promovendo um maior interesse no aprendizado, podendo favorecer análises interdisciplinares de forma lógica e objetiva, desta forma colocar a astronomia como tema motivador interdisciplinar, pode ser relevante no que se refere ao distanciamento da fragmentação dos conteúdos. No Estado de São Paulo, a implantação da proposta curricular no ensino fundamental e médio mostra claramente a inserção da astronomia na maior parte das séries, principalmente na 6ª série em que todo o bimestre se fala de astronomia.

  2. Estudo de soluções locais e cosmológicas em teorias do tipo tensor-escalar

    NASA Astrophysics Data System (ADS)

    Silva E Costa, S.

    2003-08-01

    Teorias do tipo tensor-escalar são a mais simples extensão possí vel da Relatividade Geral. Nessas teorias, cujo modelo padrão é a teoria de Brans-Dicke, a curvatura do espaço-tempo, descrita por componentes tensoriais, aparece acoplada a um campo escalar que, de certo modo, representa uma variação na constante de acoplamento da gravitação. Tais teorias apresentam soluções locais e cosmológicas que, em determinados limites, recaem nas apresentadas pela Relatividade Geral, mas que em outros limites trazem novidades, tais como conseqüências observacionais da evolução de flutuações primordiais distintas daquelas previstas pela Relatividade Geral (ver, por ex., Nagata et al., PRD 66, p. 103510 (2002)). Graças a esta possibilidade de trazer à luz novidades em relação à gravitação, teorias do tipo tensor-escalar podem ser vistas como um interessante campo alternativo de pesquisas para soluções dos problemas de massa faltante (ou escura) e/ou energia escura. Seguindo tal linha, este trabalho, ainda em sua fase inicial, apresenta soluções gerais de teorias do tipo tensor-escalar para diversas situações, verificando-se em que consiste a divergência dessas soluções dos casos tradicionais possí veis na Relatividade Geral. Como exemplos das soluções aqui apresentadas pode-se destacar uma expressão geral para diferentes soluções cosmológicas englobando diferentes tipos de matéria (representados por diferentes equações de estado), e a expressão para uma solução local representando um buraco negro com rotação, similar à solução de Kerr da Relatividade Geral. Por fim, é importante ressaltar que, embora aqui apresentem-se poucos resultados novos, na literatura sobre o assunto a maior parte das soluções apresentadas limita-se a uns poucos casos especí ficos, tal como soluções cosmológicas apenas com curvatura nula, e que mesmo as soluções disponí veis são, em geral, pouco divulgadas e, portanto, pouco conhecidas, e

  3. Estudo espectral em raios-X duros de fontes do tipo Z com o HEXTE/RXTE

    NASA Astrophysics Data System (ADS)

    D'Amico, F.; Heindl, W. A.; Rothschild, R. E.

    2003-08-01

    Apresentam-se os resultados de um estudo espectral em raios-X de fontes do tipo Z. As fontes do tipo Z são binárias de raios-X de baixa massa (BXBM) com campo magnético intermediário (B~109G). Esta classe de fontes é composta por apenas 6 fontes Galácticas (a saber: ScoX-1, 9, 7, CygX-2, 5 e 0). A nossa análise se concentra na faixa de raios-X duros (E ~ 20keV), até cerca de 200keV, faixa ótima de operação do telescópio "High Energy X-ray Timing Experiment" (HEXTE), um dos três telescópios de raios-X à bordo do Rossi X-ray Timing Explorer (RXTE). Nossa motivação para tal estudo, uma busca de caudas em raios-X duros em fontes do tipo Z, foi o pouco conhecimento sobre a emissão nesta faixa de energia das referidas fontes quando comparadas, por exemplo, as fontes do tipo atoll (também BXBM). Apresentam-se a análise/redução de dados e explicita-se a maneira como o HEXTE mede o ru1do de fundo. Especial atenção é direcionada a este item devido a localização das fontes do tipo Z e também ao problema de contaminação por fontes próximas. Com exceção de ScoX-1, nenhuma cauda em raios-X duros foi encontrada para as outras fontes, a despeito de resultados de detecção dessas caudas em algumas fontes pelo satélite BeppoSAX. As interpretações deste resultado serão apresentadas. Do ponto de vista deste estudo, nós deduzimos que a produção de caudas de raios-X duros em fontes do tipo Z é um processo disparado quando, pelo menos, uma condição é satisfeita: o brilho da componente térmica do espectro precisa estar acima de um certo valor limiar de ~4´1036ergs-1.

  4. The Conserved Splicing Factor SUA Controls Alternative Splicing of the Developmental Regulator ABI3 in Arabidopsis[W][OA

    PubMed Central

    Sugliani, Matteo; Brambilla, Vittoria; Clerkx, Emile J.M.; Koornneef, Maarten; Soppe, Wim J.J.

    2010-01-01

    ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3-α and ABI3-β, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-β transcript accumulates at the end of seed maturation. The two ABI3 transcripts differ by the presence of a cryptic intron in ABI3-α, which is spliced out in ABI3-β. The suppressor of abi3-5 (sua) mutant consistently restores wild-type seed features in the frameshift mutant abi3-5 but does not suppress other abi3 mutant alleles. SUA is a conserved splicing factor, homologous to the human protein RBM5, and reduces splicing of the cryptic ABI3 intron, leading to a decrease in ABI3-β transcript. In the abi3-5 mutant, ABI3-β codes for a functional ABI3 protein due to frameshift restoration. PMID:20525852

  5. Scheduling whole-air samples above the Trade Wind Inversion from SUAS using real-time sensors

    NASA Astrophysics Data System (ADS)

    Freer, J. E.; Greatwood, C.; Thomas, R.; Richardson, T.; Brownlow, R.; Lowry, D.; MacKenzie, A. R.; Nisbet, E. G.

    2015-12-01

    Small Unmanned Air Systems (SUAS) are increasingly being used in science applications for a range of applications. Here we explore their use to schedule the sampling of air masses up to 2.5km above ground using computer controlled bespoked Octocopter platforms. Whole-air sampling is targeted above, within and below the Trade Wind Inversion (TWI). On-board sensors profiled the TWI characteristics in real time on ascent and, hence, guided the altitudes at which samples were taken on descent. The science driver for this research is investigation of the Southern Methane Anomaly and, more broadly, the hemispheric-scale transport of long-lived atmospheric tracers in the remote troposphere. Here we focus on the practical application of SUAS for this purpose. Highlighting the need for mission planning, computer control, onboard sensors and logistics in deploying such technologies for out of line-of-sight applications. We show how such a platform can be deployed successfully, resulting in some 60 sampling flights within a 10 day period. Challenges remain regarding the deployment of such platforms routinely and cost-effectively, particularly regarding training and support. We present some initial results from the methane sampling and its implication for exploring and understanding the Southern Methane Anomaly.

  6. Estudo em microondas do aprisionamento e precipitação de elétrons em explosões solares

    NASA Astrophysics Data System (ADS)

    Rosal, A. C.; Costa, J. E. R.

    2003-08-01

    Uma explosão solar é uma variação rápida e intensa do brilho que ocorre nas chamadas regiões ativas da atmosfera, constituídas por um plasma magnetizado com intensa indução magnética. Os modelos de explosões solares atuais, discutidos na literatura, apresentam características de aprisionamento e precipitação de elétrons em ambientes magnéticos simplificados. Neste trabalho, nos propusemos a separar a emissão dos elétrons aprisionados da emissão dos elétrons em precipitação apenas a partir da emissão em microondas, melhorando portanto o controle sobre o conjunto de parâmetros inferidos. A emissão em microondas da população em precipitação é bastante fraca e portanto da nossa base de dados de 130 explosões observadas pelo Rádio Polarímetro de Nobeyama, em sete freqüências, apenas para 32 foi possível separar as duas componentes de emissão com uma boa razão sinal/ruído. A partir de estudos das escalas de tempo das emissões devidas à variação gradual da emissão no aprisionamento e da variação rápida da emissão dos elétrons em precipitação foi possível obter a separação utilizando um filtro temporal nas emissões resultantes. Em nossa análise destas explosões estudamos os espectros girossincrotrônicos da emissão gradual, a qual associamos provir do topo dos arcos magnéticos e da emissão de variação rápida associada aos elétrons em precipitação. Estes espectros foram calculados e dos quais inferimos que a indução magnética efetiva do topo e dos pés foi em média, Btopo = 236 G e Bpés = 577 G, inferidas das freqüências de pico dos espectros em ntopo = 11,8 GHz e npés = 14,6 GHz com leve anisotropia (pequeno alargamento espectral). O índice espectral da distribuição não-térmica de elétrons d, inferido do índice espectral de fótons da emissão em regime opticamente fino, foi de dtopo = 3,3 e dpés = 3,9. Estes parâmetros são típicos da maioria das análises realizadas em ambiente único de

  7. Lunar Phases and Earthly Events: Beliefs from Different Education Levels. (Spanish Title: Fases de la Luna y Acontecimientos Terrestres: Creencia de Distintos Niveles de Instrucción.) As Fases da Lua e os Acontecimentos Terrestres: a Crença de Diferentes Níveis de Instrução

    NASA Astrophysics Data System (ADS)

    Darroz, Luiz Marcelo; da Rosa, Cleci Teresinha Werner; Alves Vizzotto, Patrick; Becker da Rosa, Álvaro

    2013-12-01

    pesquisa realizada no primeiro semestre de 2013 junto a um grupo de 80 sujeitos de diferentes níveis de instrução. Nesta pesquisa, buscou-se averiguar os acontecimentos terrestres que esse grupo de pessoas atribui ao fenômeno das fases lunares. Como instrumento de coleta de dados, foram empregadas entrevistas semiestruturadas guiadas por questões que buscavam manter a atenção dos entrevistados no objeto de investigação. As entrevistas foram gravadas e transcritas, e os resultados, após serem analisados quanti e qualitativamente, foram confrontados com estudos científicos da área. Os dados da pesquisa demonstram que a Lua e suas fases continuam fascinando e despertando o interesse da população. No entanto, a falta de conhecimentos para proferir explicações corretas relativas aos fenômenos que ocorrem com o astro acaba originando uma série de crenças na população sobre sua influência nos acontecimentos terrestres.

  8. Teaching of Astronomy: Scenarios of Teaching Practice in Elementary Schools. (Spanish Title: Enseñanza de la Astronomía: Semblanzas de la Práctica Docente en Educación Primaria.) Ensino de Astronomia: Cenários da Prática Docente no Ensino Fundamental

    NASA Astrophysics Data System (ADS)

    Marchi Gonzatti, Sônia Elisa; Spessatto De Maman, Andréia; Fernandes Borragini, Eliana; Kerber, Júlia Cristina; Haetinger, Werner

    2013-12-01

    atomización de los asuntos, aunque temas como movimientos de la Tierra y fenómenos astronómicos hayan sido citados por la mayoría de los participantes. En cuanto a las estrategias, se verifico que se emplean principalmente clases con el apoyo de recursos bidimensionales, como películas, textos, mapas y búsqueda en internet. Las principales dificultades fueron la falta de formación específica con respecto a los contenidos de Astronomía, y el nivel de abstracción exigido por los contenidos. De modo general, esos indicadores convergen para los resultados ya encontrados en otros estudios, en el que las deficiencias de la formación inicial de los profesores, o incluso su ausencia, dificulta el adecuado abordaje de la Astronomía en las clases. Neste trabalho são apresentados os principais resultados de uma investigação realizada no campo da Educação em Astronomia, com professores do ensino fundamental de duas regiões do Rio Grande do Sul. O objetivo do estudo foi caracterizar o cenário regional do ensino de Astronomia, estabelecendo um comparativo com o cenário nacional. Esse estudo abordou três questões: identificar os principais temas de astronomia trabalhados em sala de aula, as estratégias metodológicas e quais as dificuldades apresentadas pelos professores ao desenvolver sua prática. Quanto aos conteúdos, encontrouse uma pulverização dos assuntos abordados, embora temas como movimentos da Terra e fenômenos astronômicos tenham sido citados pela maioria dos participantes. Sobre estratégias, são utilizadas principalmente aulas com apoio de recursos bidimensionais, como filmes, textos, mapas e pesquisa na internet. As principais dificuldades foram: falta de formação específica em relação a conteúdos de Astronomia; nível de abstração dos conteúdos, que dificultam sua compreensão, tanto pelos alunos quanto pelos próprios professores. De maneira geral, esses indicadores convergem para resultados já encontrados em outros estudos que

  9. Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of Streptococcus uberis isolated from geographically diverse areas.

    PubMed

    Yuan, Ying; Dego, Oudessa Kerro; Chen, Xueyan; Abadin, Eurife; Chan, Shangfeng; Jory, Lauren; Kovacevic, Steven; Almeida, Raul A; Oliver, Stephen P

    2014-12-01

    The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.

  10. Evaluation of the Raven sUAS to detect and monitor greater sage-grouse leks within the Middle Park population

    USGS Publications Warehouse

    Hanson, Leanne; Holmquist-Johnson, Christopher L.; Cowardin, Michelle L.

    2014-01-01

    The Raven sUAS is a hand-launched reconnaissance and data-gathering tool developed for the U.S. Department of Defense by AeroVironment, Inc. Originally designed to provide aerial observation, day or night, at line-of-site ranges up to 6.2 miles (10 kilometers), the Raven sUAS has a wingspan of 4.5 feet (1.38 meters) and weighs 4.2 pounds (1.9 kilograms). A 60-minute lithium-ion rechargeable battery powers the system which also transmits live video (color or infrared imagery), compass headings, and location information to a ground control station. The Raven sUAS is typically operated by a three-person flight crew consisting of a pilot, mission operator, and a trained observer.

  11. Estudo e aplicação do código de desembaraçamento espectral - Korel - em sistemas triplos

    NASA Astrophysics Data System (ADS)

    Torres, K. B. V.; Vaz, L. P. R.

    2003-08-01

    O código KOREL é uma ferramenta adicional eficiente para a determinação simultânea de parâmetros orbitais espectroscópicos para o desembaraço de espectros individuais de sistemas estelares com até cinco componentes. Estudos já realizados (Torres,KBV,2002, Dissertação de Mestrado DF-ICEx-UFMG) mostram as limitações, aplicabilidade e eficiência do código para sistemas com duas componentes. Apresentamos, neste trabalho, a continuação dos estudos para sistemas com três componentes (sistemas hierárquicos), utilizando espectros sintéticos "gabaritos" de estrelas individuais em órbitas cujos parâmetros são conhecidos "a priori". Geramos os espectros compostos e verificamos como o código reproduz os parâmetros orbitais e espectros individuais em diversas situações físicas. É apresentado, ainda, uma discussão da aplicabilidade do código em sistemas reais como RV Crateris (Machado,ACM,1997, Dissertação de Mestrado DF-ICEx-UFMG), um sistema triplo cujas componentes eclipsantes estão, provavelmente, ainda na fase pré-sequência principal.

  12. Raízes pós-mitológicas da Astronomia Clássica

    NASA Astrophysics Data System (ADS)

    di Bartolo, L.; Villas da Rocha, J. F.

    2004-02-01

    Apresentamos como a invenção de um modelo astronômico e cosmológico fundado em causas naturais foi um processo gestado num ambiente cultural específico, o do advento do pensamento pós-mitológico, que tem como sua raiz mais funda a trajetória particular da Grécia Antiga, com ênfase na resolução de uma grave crise social e a evolução do mundo clássico para o contexto espiritual de um império universal.

  13. Henrique da Rocha Lima*

    PubMed Central

    Bernardes Filho, Fred; Avelleira, João Carlos Regazzi

    2015-01-01

    Brazilian physician and researcher Henrique da Rocha Lima was born in 1879 in the city of Rio de Janeiro, where he studied medicine and obtained the degree of M.D. in 1901. He specialized in Clinical Medicine in Germany and was the ambassador in European countries of the scientific medicine that emerged from the Oswaldo Cruz Institute in the early twentieth century. Rocha Lima has discovered the causative agent of typhus and had a major contribution to the studies of yellow fever, Chagas disease, Carrión’s disease and histoplasmosis. His genius, his research and his discoveries projected his name, and, with it, the image of Brazil in the international scientific scene. PMID:26131867

  14. Difficulties of First Years Elementary School Teachers with the Teaching of Astronomy. (Breton Title: Dificuldades de Professores dos Anos Iniciais do Ensino Fundamental em Relação ao Ensino da Astronomia. ) Dificultades de LOS Profesores de los Primeros Años de la Escuela Primaria en Relación a la Enseñanza de la Astronomía

    NASA Astrophysics Data System (ADS)

    Langhi, Rodolfo; Nardi, Roberto

    2005-12-01

    programa de formação continuada neste tema. O estudo se justifica mediante o fato de que planejamentos de cursos como estes só se adequarão à realidade do professor (e do aluno) se houver uma investigação antecipada sobre o que os docentes precisam saber e saber fazer a respeito da Astronomia, o que se concretizou em nosso caso pela interpretação dos discursos de uma amostra de professores coletados através de entrevistas semi-estruturadas, utilizando para interpretação os princípios e métodos da análise do discurso em sua linha francesa. Os resultados da pesquisa indicaram dificuldades de ordem pessoal, metodológica, de formação, de infra-estrutura e outras relacionadas às fontes de informações para docentes. Este artículo que relata las dificultades de los profesores en relación a la enseñanza da laAstronomía es parte de un estudio preliminar para la implantación dela Astrnomía enla formación de profesores de ls primeros años del ciclo primario.El estudio considera las concepciones alternativas de alumnos y profesores respecto a los fenómenos astronómicos, los errores conceptuales en los libros didácticos y las sugerencias de contenidos de Astronomía que constan en los Parámetros Curriculares Nacionales del Brasil. Caracterizar las dificultades de los profesores constituye la cuestión central de este texto, apuntando para el objetivo de contribuir para un futuro programa de educación contínua en este tema. El estudio se justifica mediante el hecho que la planificación de cursos de este tipo solo se adecuarán a la realidade del profesor (y del alumno) si existe una investigación anterior a respecto de lo que los docentes precisan saber y saber realizar en Astronomía, lo cual se concretó en nuestro caso por medio de la interpretación de los discursos de una muestra de profesores obtenidos através de entrevistas semiestructuradas, utilizand para esta interpretación los principios y métodos de análisis del discurso en su l

  15. Difficulties of First Years Elementary School Teachers with the Teaching of Astronomy. (Breton Title: Dificuldades de Professores dos Anos Iniciais do Ensino Fundamental em Relação ao Ensino da Astronomia. ) Dificultades de LOS Profesores de los Primeros Años de la Escuela Primaria en Relación a la Enseñanza de la Astronomía

    NASA Astrophysics Data System (ADS)

    Langhi, Rodolfo; Nardi, Roberto

    2005-12-01

    programa de formação continuada neste tema. O estudo se justifica mediante o fato de que planejamentos de cursos como estes só se adequarão à realidade do professor (e do aluno) se houver uma investigação antecipada sobre o que os docentes precisam saber e saber fazer a respeito da Astronomia, o que se concretizou em nosso caso pela interpretação dos discursos de uma amostra de professores coletados através de entrevistas semi-estruturadas, utilizando para interpretação os princípios e métodos da análise do discurso em sua linha francesa. Os resultados da pesquisa indicaram dificuldades de ordem pessoal, metodológica, de formação, de infra-estrutura e outras relacionadas às fontes de informações para docentes. Este artículo que relata las dificultades de los profesores en relación a la enseñanza da laAstronomía es parte de un estudio preliminar para la implantación dela Astrnomía enla formación de profesores de ls primeros años del ciclo primario.El estudio considera las concepciones alternativas de alumnos y profesores respecto a los fenómenos astronómicos, los errores conceptuales en los libros didácticos y las sugerencias de contenidos de Astronomía que constan en los Parámetros Curriculares Nacionales del Brasil. Caracterizar las dificultades de los profesores constituye la cuestión central de este texto, apuntando para el objetivo de contribuir para un futuro programa de educación contínua en este tema. El estudio se justifica mediante el hecho que la planificación de cursos de este tipo solo se adecuarán a la realidade del profesor (y del alumno) si existe una investigación anterior a respecto de lo que los docentes precisan saber y saber realizar en Astronomía, lo cual se concretó en nuestro caso por medio de la interpretación de los discursos de una muestra de profesores obtenidos através de entrevistas semiestructuradas, utilizand para esta interpretación los principios y métodos de análisis del discurso en su l

  16. A cosmologia no ensino da geografia

    NASA Astrophysics Data System (ADS)

    Santos, S. C.; Chiaradia, A. P. M.

    2003-08-01

    O principal objetivo deste trabalho é auxiliar o professor de Geografia em sala de aula no ensino de tópicos relacionados com a Cosmologia. A idéia deste trabalho surgiu quando foi constatado que o professor de Geografia tem dificuldades de ensinar este tópico. Esta constatação foi feita por uma das autoras ao lecionar este tópico no ensino fundamental e em discussões com outros professores de Geografia. Da mesma maneira que ocorria desde os tempos mais antigos, os alunos têm muito interesse em conhecer os fenômenos que ocorrem no Cosmo, porém os livros didáticos de Geografia utilizados em sala de aula não são ricos em informações sobre este assunto. Assim, o professor de Geografia tem poucas informações para discutir este assunto em sala de aula e não dá a devida importância para este tópico. Então, foi desenvolvido um material de apoio para professores de Geografia sobre a origem do Universo, sua evolução e seu possível futuro evolutivo segundo as mais recentes teorias, com base em perguntas feitas pelos alunos de ensino fundamental e as informações trazidas nos livros didáticos Não cabe a este material inovar e tão pouco trazer uma metodologia de ensino de Cosmologia. Neste material o professor de Geografia pode encontrará um banco de informações, que constitui no estabelecimento de conceitos, teorias e hipóteses, sobre a Cosmologia, em linguagem simples e de fácil entendimento. Para desenvolvê-lo, foram feitas pesquisas não exaustivas em livros e revistas científicas, compilação e discussão em forma cronológica das teorias aceitas sobre modelos cosmológicos. Portanto, este material será apresentado neste trabalho.

  17. Análise da aplicação e dos resultados do modelo OPM3® para a área da saúde

    PubMed Central

    Augusto dos Santos, Luis; de Fátima Marin, Heimar

    2015-01-01

    Esta pesquisa procurou analisar se um modelo de questionário criado por uma comunidade internacional de gerenciamento de projetos e se é aplicavél a organizações voltadas a área de saúde. O modelo OPM3® (Organizational Project Management Maturity Model) foi criado para que organizações de qualquer área ou porte pudessem identificar a presença, ou ausência, de boas práticas de gerenciamento. O objetivo da aplicação desse modelo é avaliar sempre a organização e não o entrevistado. No presente artigo, são apresentados os resultados da aplicação desse modelo em uma organização que possuía produtos e serviços de tecnologia da informação aplicados à área de saúde. Este estudo verificou que o modelo é aplicável de forma rápida e que a organização analisada possuía um número expressivo de boas práticas. PMID:26924862

  18. Leonardo da Vinci and the Downburst.

    NASA Astrophysics Data System (ADS)

    Gedzelman, Stanley David

    1990-05-01

    Evidence from the drawings, experiments, and writings of Leonardo da Vinci are presented to demonstrate that da Vinci recognized and, possibly, discovered the downburst and understood its associated airflow. Other early references to vortex flows resembling downbursts are mentioned.

  19. Sobre a largura da última superfície de espalhamento

    NASA Astrophysics Data System (ADS)

    Nobre, M. A. S.; Pires, N.; Lima, J. A. S.

    2003-08-01

    De acordo com o modelo do "Big-Bang", no universo primordial a matéria estava em equilíbrio térmico com a radiação. Com a expansão a temperatura da radiação cai. Quando a temperatura chega em torno dos 4.000K, os espalhamentos diminuem, começando a recombinação dos prótons e elétrons em Hidrogênio neutro (era conhecida como da recombinação). Ao final da recombinação, os fótons se propagam livremente sofrendo, em princípio, somente os efeitos do "redshift" cosmológico. Esses fótons nos alcançam hoje como a radiação cósmica de fundo (RCF), e parecem vir de uma superfície esférica ao nosso redor, tal que o raio dela é a distância que ele viajou desde seu último espalhamento na época da recombinação. Naturalmente, esse processo não ocorreu abruptamente, implicando na existência de uma largura no espaço dos "redshifts" que deve depender do modelo cosmológico específico e dos processos físicos considerados. Neste trabalho analisamos os efeitos de diferentes modelos - a saber, aqueles com decaimento do vácuo L(t), criação de matéria, quintessência e gás de Chaplygin - sobre a última superfície de espalhamento da RCF, em particular sua largura e a função visibilidade, que determina a probabilidade de um fóton ter tido seu último espalhamento num "redshift" z. No caso particular dos modelos com decaimento do vácuo, existe uma forte dependência da função visibilidade com L(t). Tais efeitos poderão ser testados através da análise dos resultados de experimentos mais precisos que estão atualmente em andamento, como por exemplo, o WMAP.

  20. The major histocompatibility complex genes impact pain response in DA and DA.1U rats.

    PubMed

    Guo, Yuan; Yao, Fan-Rong; Cao, Dong-Yuan; Li, Li; Wang, Hui-Sheng; Xie, Wen; Zhao, Yan

    2015-08-01

    Our recent studies have shown that the difference in basal pain sensitivity to mechanical and thermal stimulation between Dark-Agouti (DA) rats and a novel congenic DA.1U rats is major histocompatibility complex (MHC) genes dependent. In the present study, we further used DA and DA.1U rats to investigate the role of MHC genes in formalin-induced pain model by behavioral, electrophysiological and immunohistochemical methods. Behavioral results showed biphasic nociceptive behaviors increased significantly following the intraplantar injection of formalin in the hindpaw of DA and DA.1U rats. The main nociceptive behaviors were lifting and licking, especially in DA rats (P<0.001 and P<0.01). The composite pain scores (CPS) in DA rats were significantly higher than those in DA.1U rats in both phases of the formalin test (P<0.01). Electrophysiological results also showed the biphasic increase in discharge rates of C and Aδ fibers of L5 dorsal root in the two strains, and the net change of the discharge rate of DA rats was significantly higher than that of DA.1U rats (P<0.05). The mechanical thresholds decreased after formalin injection in both strains (P<0.01), and the net change in the mechanical threshold in DA was greater than that in DA.1U rats (P<0.05). The expression of RT1-B, representation of MHC class II molecule, in laminae I-II of L4/5 spinal cord in DA rats was significantly higher than that in DA.1U rats in the respective experimental group (P<0.05). These results suggested that both DA and DA.1U rats exhibited nociceptive responses in formalin-induced pain model and DA rats were more sensitive to noxious chemical stimulus than DA.1U rats, indicating that MHC genes might contribute to the difference in pain sensitivity.

  1. A Proposed Activity for a Meaningful Learning about the Moon Phases. (Breton Title: Uma Proposta de Atividade Para a Aprendizagem Significativa sobre as Fases da Lua.) Una Actividad Propuesta Para EL Aprendizaje Significativo Acerca de Las Fases de la Luna

    NASA Astrophysics Data System (ADS)

    Martins, Bruno Andrade; Langhi, Rodolfo

    2012-12-01

    This paper presents one of the concepts of Astronomy and its consequent failure in teaching this topic in high school, even when the official documents point out the necessity of Astronomy teaching at this school level. Among the spontaneous conceptions in Astronomy that high school students carry with them, even after the end of the school, we emphasized in this research the Moon phases. The development of different strategies in relation to traditional methods, aimed to teaching-learning process on this topic was considered in this study. These strategies were devised based on the reference frame of the Meaningful Learning, as elaborated by Ausubel. The proposals presented here include the active participation of students in experimental activities and other didactic activities, for their continuous evaluation during the process. These activities finished with a Comics elaboration about the Moon phases. Therefore, the objective of this paper is to present a proposal for differentiated teaching activity about Moon phases supported by the theoretical principles of Meaningful Learning at Physics classes. Este texto foca um dos conteúdos de Astronomia e a consequente falha no ensino deste tema no ensino médio, apesar de os documentos oficiais apresentarem a necessidade de se trabalhar a Astronomia neste nível de ensino. Dentre as concepções alternativas em Astronomia que os alunos do ensino médio carregam consigo, mesmo após o término dos estudos, destacamos, nesta pesquisa, o fenômeno das fases da Lua. O desenvolvimento de estratégias diferenciadas em relação ao ensino tradicional, visando o processo de ensino-aprendizagem sobre este tema, foi contemplado neste trabalho como um dos resultados obtidos sob a luz dos referenciais da aprendizagem significativa, fundamentados em Ausubel. Segundo a proposta aqui apresentada, a participação ativa dos alunos na execução de uma atividade experimental e outras atividades didáticas, que visam sua cont

  2. Estudo da Oscilação de Neutrinos Muônicos Usando Dados Atmosféricos e de Acelerador nos Experimentos MINOS e MINOS+

    SciTech Connect

    Medeiros, Michelle Mesquita de

    2015-01-01

    The MINOS (Main Injector Neutrino Oscillation Search) and MINOS+ experiments were designed to study neutrino oscillations using a muon neutrino beam which is detected in two different locations, in the Near Detector and in the Far Detector. The distance between the detectors allows the beam neutrinos to oscillate to a different flavor. Therefore, a disappearance of the muon neutrinos from the beam is observed in the Far Detector. The Far Detector has a special apparatus which makes possible the selection of atmospheric neutrinos and antineutrinos. These come from interactions of cosmic rays with the Earth’s atmosphere. Both detectors have a magnetic field, allowing the distiction between neutrinos and antineutrinos interactions. This thesis presents the first combined analysis of data from the MINOS and MINOS+ experiments. We have analyzed the combined neutrino energy spectrum from the complete MINOS beam data and the first, more energetic, MINOS+ beam data. The disappearance of the muon neutrinos was observed and the data has shown to be congruent with the oscillation model. Beyond that, we have measured the atmospheric oscillation parameters of the beam and atmospheric neutrinos and antineutrinos from MINOS combined with the atmospheric neutrinos and antineutrinos from MINOS+. Assuming the same oscillation parameters for both neutrinos and antineutrinos, the best fit is obtained for inverted hierarchy and lower octant with Δm2 32 = 2:37 X 10-3 eV2 and sin2 θ 23 = 0:43, and the limits m2 32 = [2,29 - 2,49] 10-3 eV2 (68%) and sin2 θ23 = 0.36 - 0.66 (90%). These results are the most precise measurement of the neutrinos mass splitting using muon neutrino disappearance data only.

  3. Virtual observatory publishing with DaCHS

    NASA Astrophysics Data System (ADS)

    Demleitner, M.; Neves, M. C.; Rothmaier, F.; Wambsganss, J.

    2014-11-01

    The Data Center Helper Suite DaCHS is an integrated publication package for building VO and Web services, supporting the entire workflow from ingestion to data mapping to service definition. It implements all major data discovery, data access, and registry protocols defined by the VO. DaCHS in this sense works as glue between data produced by the data providers and the standard protocols and formats defined by the VO. This paper discusses central elements of the design of the package and gives two case studies of how VO protocols are implemented using DaCHS' concepts.

  4. Provenance analysis and thermo-dynamic studies of multi-type Holocene duricrusts (1700 BC) in the Sua Salt Pan, NE Botswana

    NASA Astrophysics Data System (ADS)

    Dill, Harald G.; Dohrmann, R.; Kaufhold, S.; Techmer, A.

    2014-08-01

    Multi-type duricrusts, composed of silcretes, calcretes, halcretes and sulcretes developed during the Holocene at the northern rim of the Sua Salt Pan, NE Botswana. They were investigated for their light (quartz/chalcedony, feldspar, analcime, clinoptilolite, calcite, kaolinite/halloysite, illite-smectite mixed-layers, halite) and heavy minerals (baryte, clinozoisite-epidote s.s.s., amphibole, corundum, tourmaline, ilmenite, rutile, sphene, kyanite, andalusite, staurolite, garnet, zircon, apatite, monazite, cassiterite, garnet, biotite) using petrographic microscopy, X-ray fluorescence and diffraction analyses, radio-carbon dating, scanning electron microscopy equipped with an EDX-system, cation exchange capacity and infrared spectroscopy. Detrital minerals predominantly derived from the erosion of rocks belonging to the Archaean Basement Complex, the Stormberg Volcanites and the Kalahari sediments. Of particular interest to exploration geologists, geikielite-enriched ilmenite fragments are a hint to kimberlitic pipes. Biodetritus was derived from invertebrates and from vertebrates (fish bones?). A man-made impact on the heavy mineral suite has to be invoked from small fragments of cassiterite fragments that derived from processing of sulfidic and pegmatitic Sn-bearing ore. In the salt-pan-derived duricrusts mainly the aeolian and to a lesser degree fluvial inputs were responsible for the concentration of clasts in these multi-type duricrusts. Moreover, their variegated mineralogy enables us to constrain the physical-chemical regime, prevalently as to the pH and the chemical composition of the major constituents. All duricrusts developed in a self-sufficient chemically closed system where quartz and feldspar provided the elements Si, Na, K, Ca, and Ba to produce the encrustations. The spatial and temporal trend in the Sua Salt Pan rim encrustations may be described as follows: (1) sulcrete-silcretes, (2) silcretes with kaolinite-group minerals towards more recent

  5. Origin of the DA and non-DA white dwarf stars

    NASA Technical Reports Server (NTRS)

    Shipman, Harry L.

    1989-01-01

    Various proposals for the bifurcation of the white dwarf cooling sequence are reviewed. 'Primordial' theories, in which the basic bifurcation of the white dwarf sequence is rooted in events predating the white dwarf stage of stellar evolution, are discussed, along with the competing 'mixing' theories in which processes occurring during the white dwarf stage are responsible for the existence of DA or non-DA stars. A new proposal is suggested, representing a two-channel scenario. In the DA channel, some process reduces the hydrogen layer mass to the value of less than 10 to the -7th. The non-DA channel is similar to that in the primordial scenario. These considerations suggest that some mechanism operates in both channels to reduce the thickness of the outermost layer of the white dwarf. It is also noted that accretion from the interstellar medium has little to do with whether a particular white dwarf becomes a DA or a non-DA star.

  6. A distância e o conteúdo estelar da região HII gigante G333.1-0.4 - vínculos para a taxa de formação estelar da galáxia

    NASA Astrophysics Data System (ADS)

    Figuerêdo, E.; Damineli, A.; Blum, R.; Conti, P.

    2003-08-01

    Neste trabalho apresentamos imagens de alta resolução angular da região HII gigante G333.1-0.4 obtidas através dos filtros J, H e K no telescópio de 4-m do CTIO. Este trabalho faz parte de um estudo de regiões HII gigantes no infravermelho próximo que tem por objetivo estudar a natureza da formação de estrelas massivas e traçar a estrutura espiral de nossa galáxia. Nossa determinação da distância é baseada no método da paralaxe espectroscópica de estrelas OB localizadas na seqüência principal de idade zero (ZAMS) do Diagrama HR. No caso de G333.1-0.4, a magnitude aparente das estrelas localizadas na ZAMS indica que a distância não pode ser maior do que o limite inferior determinado por técnica rádio (2,8 kpc). Resultados semelhantes foram encontrados para regiões estudadas anteriormente, reforçando a idéia de que a taxa de formação estelar na Via Láctea é menor do que o determinado a partir de dados rádio. Nossos resultados mais recentes sobre o conteúdo estelar de G333.1-0.4 revelaram vários objetos que possuem cores bastante avermelhadas (H-K > 2,0). Nós identificamos estes objetos usando os diagramas cor-cor e cor-magnitude dos aglomerados. Estes objetos apresentam um forte excesso em emissão na banda K e possivelmente se tratam de estrelas do tipo OB envolvidas por um disco/envelope circumestelar espesso. O estudo da função de massa inical desta região, em conjunto com resultados de nossos trabalhos anteriores, aponta para uma IMF independente da posição galática. A contagem de estrelas nos fornece um valor para o número de fótons no contínuo de Lyman que corrobora com a afirmação de que G333.1-0.4 se encontra mais próxima da menor distância determinada por rádio.

  7. Implementação de um algoritmo para a limpeza de mapas da RCFM

    NASA Astrophysics Data System (ADS)

    Souza, C. L.; Wuensche, C. A.

    2003-08-01

    A Radiação Cósmica de Fundo em Microondas (RCFM), descoberta por Penzias e Wilson em 1965, é uma das ferramentas mais poderosas para o estudo da cosmologia. Com a descoberta de flutuações de temperatura na RCFM, da ordem de uma parte em 105, pelo COBE (1992), uma nova era teve início. Nos últimos onze anos, diversos instrumentos fizeram novas medidas de alta precisão, refinando os resultados apresentados pelo COBE, culminando com os resultados recentes do satélite WMAP. A análise de dados da RCFM, especialmente no caso de experimentos com pequena cobertura do céu, apresenta uma série de dificuldades devido a emissões de contaminantes externos, tais como a emissão da Galáxia e de fontes pontuais, e de ruídos intrínsecos tanto ao sistema de detecção quanto à estratégia de observação do céu. Uma das soluções típicas para a filtragem de dados brutos de um experimento para medir flutuações de temperatura é aplicar um gabarito (template) e um filtro passa alta ao produzir mapas simplificados (sem considerar matrizes de correlação ou covariância). No caso de experimentos que utilizam detectores HEMT, essa combinação de filtros remove, satisfatoriamente, ruídos do tipo 1/f gerados pela instabilidade no ganho do detector acoplado ao movimento do instrumento, definido pela estratégia de observação. Entretanto, o sinal resultante medido, tanto em simulações quanto em séries temporais reais, sugere que parte do sinal cosmológico pode estar sendo removido junto com o ruído dos detectores. Este trabalho descreve as etapas para a produção de um mapa típico (simulado) e os testes preliminares de um algoritmo para remover ruídos do tipo 1/f introduzidos pela estratégia de observação sem prejudicar a qualidade do sinal cosmológico presente no mapa.

  8. Estudo da utilizacao da biomassa florestal como fonte de produtos quimicos e energia atraves de processos quimicos e biotechnologicos (Utilization of forest biomass for chemicals and energy by chemical and biotechnological processes). Master's thesis

    SciTech Connect

    de Miranda, I.M.S.S.

    1989-04-01

    The chemical composition and the non-structural carbohydrate composition were determined for 1-year-old Eucalyptus globulus Labill trees, grown in an experimental plantation with different nutrient and water supplies (C-trees, where no treatment was made and IL-trees, which received a nutrient supply and irrigation). Leaf carbohydrates were determined for the same conditions. Pulping experiments were made using kraft cooks of eucalyptus wood. The results of the pretreatments are complemented with scanning electron microscopy observations. Average wood composition was: ash 1.6%, total extractives 5.2%, Klason lignin 21.0%, soluble lignin 3.1% (% of o.d. weight). The results did not show statistical significant differences between the two growth conditions. The average wood carbohydrate composition was: glucose 2.7%, frutose 4.1% sucrose 1.3% and starch 0.5%. The average carbohydrate composition of leaves was: glucose 1.0%, frutose 1.7%, sucrose 0.1% and starch 4.1%. The average yield for the kraft wood delinification was 49%. The saccharification of pretreated eucalyptus chips with a steam-explosion pretreatment was: For H2SO4 impregnation 26.6% and for H2O impregnation 44.2%.

  9. The Supernova Impostor SN 2010da

    NASA Astrophysics Data System (ADS)

    Binder, Breanna A.; Williams, Benjamin F.; Kong, Albert K. H.; Plucinsky, Paul P.; Gaetz, Terrance J.; Skillman, Evan D.; Dolphin, Andrew E.

    2016-01-01

    Supernova impostors are optical transients that, despite being assigned a supernova designation, do not signal the death of a massive star or accreting white dwarf. Instead, many impostors are thought to be major eruptions from luminous blue variables. Although the physical cause of these eruptions is still debated, tidal interactions from a binary companion has recently gained traction as a possible explanation for observations of some supernova impostors. In this talk, I will discuss the particularly interesting impostor SN 2010da, which exhibits high-luminosity, variable X-ray emission. The X-ray emission is consistent with accretion onto a neutron star, making SN 2010da a likely high mass X-ray binary in addition to a supernova impostor. SN 2010da is a unique laboratory for understanding both binary interactions as drivers of massive star eruptions and the evolutionary processes that create high mass X-ray binaries.

  10. A geometria do campo magnético na região da nuvem Lupus 1

    NASA Astrophysics Data System (ADS)

    Alves, F. P.; Franco, G. A. P.

    2003-08-01

    Apresentaremos os resultados de uma investigação polarimétrica na região de formação estelar junto à nuvem escura Lupus 1. Esse estudo baseia-se em polarimétria CCD obtida na banda R, e cobre Lupus 1, bem como a área vizinha a essa nuvem contendo a cavidade em 100 mm IRAS. Os dados observacionais foram coletados com o telescópio IAG de 60 cm do Observatório do Pico dos Dias (LNA/MCT - Brasópolis - MG). Nossa primeira análise mostra que uma variação da orientação do campo magnético através da região pode produzir padrões complexos de polarização cuja geometria do campo não pode ser facilmente determinada. Os padrões de polarização são inconsistentes com um campo magnético estritamente uniforme e unidimensional em larga escala. Comparação com a emissão em 100 mm mostra que localmente os vetores de polarização exibem um forte alinhamento com a orientação dos padrões observados em infravermelho.

  11. The local effect of octreotide on mechanical pain sensitivity is more sensitive in DA rats than DA.1U rats.

    PubMed

    Yao, Fan-Rong; Wang, Hui-Sheng; Guo, Yuan; Zhao, Yan

    2016-02-01

    A recent study by the authors indicated that major histocompatibility complex (MHC) genes are associated with the differences in basal pain sensitivity and in formalin model between Dark-Agouti (DA) and novel congenic DA.1U rats, which have the same genetic background as DA rats except for the u alleles of MHC. The objective of the present study is to investigate whether there is a difference in the pristane-induced arthritis (PIA) model and local analgesic effect of octreotide (OCT) between DA and DA.1U rats. The hindpaw mechanical withdrawal threshold (MWT) and heat withdrawal latency (HWL) were observed. The C unit firings of the tibial nerve evoked by non-noxious and noxious toe movements were recorded by electrophysiological methods in normal and PIA models in DA and DA.1U rats before and after local OCT administration. The expression of somatostatin receptor 2A (SSTR2A) was observed by immunohistochemistry. The results demonstrate that DA rats have a higher mechanical sensitivity than DA.1U rats after PIA. Local OCT administration significantly elevated MWT in DA rats under normal and PIA sate, but not in DA.1U rats. The electrophysiological experiments showed OCT significantly attenuated the firings of C units evoked by non-noxious and noxious stimulation in DA rats more than those in DA.1U rats both in normal and PIA states. In addition, the expression of SSTR2A in the dorsal horn of the spinal cord was significantly higher in DA than in DA.1U rats. All of the findings suggest a higher local analgesic effect of OCT in DA rats than DA.1U rats, which might be associated with the MHC genes.

  12. Detecção da fase impulsiva de uma explosão solar gigante até 405 GHz

    NASA Astrophysics Data System (ADS)

    Raulin, J.-P.; Makhmutov, V.; Kaufmann, P.; Pacini, A. A.; Luethi, T.; Hudson, H. S.; Gary, D. E.; Yoshimori, M.

    2003-08-01

    A explosão ocorrida no dia 25/08/2001 foi uma das mais intensas do presente ciclo solar em ondas de rádio de altas frequências. Foram medidas em ondas milimétricas e submilimétricas, aproximadamente, 105 e vários milhares de unidades de fluxo solar, respectivamente. Apresentamos um estudo deste evento em múltiplas frequências, desde microondas (1GHz), até ondas submilimétricas (405 GHz) detectadas pelo Telescópio Solar para ondas Submilimétricas (SST). Esta base de dados foi complementada utilizando-se o experimento Yohkoh, incluindo a emissão em raios-X duros e raios-g (até 100 MeV), e imagens em raios-X moles da região ativa envolvida. Enfocamos e discutimos principalmente os seguintes aspectos da fase impulsiva do evento: (i) as implicações deduzidas do espectro eletromagnético, obtido pela primeira vez até 405 GHz; (ii) a dinâmica da região ativa. Os resultados mostram que para explicar o espectro rádio observado, são necessários entre 3.5×1037 e 1.5×1039 elétrons acelerados acima de 20 keV em uma região de campo magnético entre 300 e 800 Gauss. A estimativa do fluxo de fótons que seria produzido por estes elétrons, mostra que grande parte deles não precipitou na baixa atmosfera. A evolução temporal da emissão em raios-X moles revela que a configuração magnética da região ativa foi muito dinâmica durante a fase impulsiva da explosão. Em particular, mostramos que a produção dos elétrons altamente energéticos foi iniciada junto com a aparição, na baixa coroa solar, de um novo sistema compacto de estruturas magnéticas. Este fato sugere que os locais de aceleração estão localizados na baixa atmosfera do Sol, como resultado da interação entre o novo sistema compacto e o campo magnético ambiente da região ativa.

  13. Common colds on Tristan da Cunha

    PubMed Central

    Shibli, M.; Gooch, S.; Lewis, H. E.; Tyrrell, D. A. J.

    1971-01-01

    Eight epidemics of respiratory disease have been observed among islanders of Tristan da Cunha. They seem to be initiated by the arrival of ships and transmission seemed to occur as a result of close human contact but could not always be traced. Islanders suffered from less colds than those in less isolated communities. PMID:5282927

  14. DA White Dwarfs in the Kepler Field

    NASA Astrophysics Data System (ADS)

    Doyle, T. F.; Howell, S. B.; Petit, V.; Lépine, S.

    2016-10-01

    We present 16 new, and confirm 7 previously identified, DA white dwarfs in the Kepler field through ground-based spectroscopy with the Hale 200″, Kitt Peak 4-meter, and Bok 2.3-meter telescopes. Using atmospheric models we determine their effective temperatures and surface gravities to constrain their position with respect to the ZZ Ceti (DA pulsator) instability strip, and look for the presence or absence of pulsation with Kepler's unprecedented photometry. Our results are as follows: i) From our measurements of temperature and surface gravity, 12 of the 23 DA white dwarfs from this work fall well outside of the instability strip. The Kepler photometry available for 11 of these WDs allows us to confirm that none are pulsating. One of these eleven happens to be a presumed binary, KIC 11604781, with a period of ˜5 days. ii) The remaining 11 DA white dwarfs are instability strip candidates, potentially falling within the current, empirical instability strip, after accounting for uncertainties. These WDs will help constrain the strip's location further, as eight are near the blue edge and three are near the red edge of the instability strip. Four of these WDs do not have Kepler photometry, so ground-based photometry is needed to determine the pulsation nature of these white dwarfs. The remaining seven have Kepler photometry available, but do not show any periodicity on typical WD pulsation timescales.

  15. How to Think Like Leonardo da Vinci

    ERIC Educational Resources Information Center

    Caouette, Ralph

    2008-01-01

    To be effective and relevant in twenty-first-century learning, art needs to be more inclusive. In this article, the author discusses how teachers can find a good example in Leonardo da Vinci for building an art program. His art, design, and curiosity are the perfect foundation for any art program, at any level. (Contains 3 resources and 3 online…

  16. The PAN-DA data acquisition system

    SciTech Connect

    Petravick, D.; Berg, D.; Berman, E.; Bernett, M.; Constanta-Fanourakis, P.; Dorries, T.; Haire, M.; Kaczar, K; MacKinnon, B.; Moore, C.; Nicinski, T.; Oleynik, G.; Pordes, R.; Sergey, G.; Votava, M.; White, V.

    1989-05-01

    The Online and Data Acquisition software groups at Fermi National Accelerator Laboratory have extended the VAXONLINE data acquisition package to include a VME based data path. The resulting environment, PAN-DA, provides a high throughput for logging, filtering, formatting and selecting events. 10 refs., 1 fig.

  17. Hidden sketches by Leonardo da Vinci revealed

    NASA Astrophysics Data System (ADS)

    Dumé, Belle

    2009-02-01

    Three drawings on the back of Leonardo da Vinci's The Virgin and Child with St Anne (circa 1508) have been discovered by researchers led by Michel Menu from the Centre de Recherche et de Restauration des Musées de France (C2RMF) and the Louvre Museum in Paris.

  18. Microvariabilidade da polarização óptica e do fluxo infravermelho de 3C 279

    NASA Astrophysics Data System (ADS)

    Andruchow, I.; Cellone, S. A.; Romero, G. E.; Dominici, T. P.; Abraham, Z.

    2003-08-01

    Neste trabalho mostramos um estudo sobre a existência e o comportamento da microvariabilidade na polarização óptica no blazar 3C 279. As medidas de polarização foram feitas durante quatro noites em março/2002 com o telescópio de 2.15 m do CASLEO, utilizando o polarímetro CASPROF. As observações mostraram que o grau de polarização linear é composto por duas componentes: uma relativa ao flickering em escalas de tempo de minutos e outra com variações em escalas de tempo de dias. Neste último caso a variação do grau de polarização está correlacionada com variações intraday no infravermelho próximo, cujos dados foram obtidos simultaneamente durante duas noites no telescópio de 1.60 m do LNA, utilizando a CamIV. Além disso, o ângulo de polarização apresentou uma variação súbita de mais de 10 graus em algumas horas, o que poderia ser causado pela injeção de um novo choque no jato. Esta idéia é apoiada pelo comportamento da variabilidade no infravermelho em escalas de tempo mais longas, que foi acompanhado durante três meses após o término da campanha coordenada com observações adicionais no LNA.

  19. 32 CFR 516.25 - DA Form 4.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true DA Form 4. 516.25 Section 516.25 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF CIVIL AUTHORITIES AND PUBLIC RELATIONS LITIGATION Reporting Legal Proceedings to HQDA § 516.25 DA Form 4. (a) General. The DA Form 4 (See figure...

  20. A Day in the Life at DaVita Academy

    ERIC Educational Resources Information Center

    Weinstein, Margery

    2010-01-01

    When a company name means "giving life," the bar for learning and development programs is held high. In this article, the author describes what it takes to graduate from DaVita Academy, the soft skills training program dialysis services company DaVita offers all its employees. DaVita's chief executive officer, Kent Thiry, states that the Academy…

  1. Análise de Estruturas Morfológicas da Cauda de Plasma do Cometa P/Halley

    NASA Astrophysics Data System (ADS)

    Voelzke, M. R.; Matsuura, O. T.

    1997-08-01

    531 imagens contidas no The International Halley Watch Atlas of Large-Scale Phenomena (Brandt et al., 1992) cobrindo o período de setembro de 1985 a julho de 1986 foram analisadas visando identificar, caracterizar as propriedades e correlacionar estruturas morfológicas da cauda de plasma do cometa P/Halley. A análise revelou 151 estruturas ondulatórias, 146 ondas solitárias (sólitons), 12 caudas do tipo Swan (Hyder et al., 1974; Niedner & Brandt, 1980; Jockers, 1985), 47 eventos de desconexão (D.E.'s) (Niedner & Brandt, 1979; Jockers, 1985; Celnik et al., 1988; Delva et al., 1991) e 23 regiões de adensamento ("knots") (Matsuura & Voelzke, 1990; Voelzke, 1996). - feita uma análise comparativa com outros trabalhos similares objetivando disciplinar a nomenclatura das estruturas morfológicas e a sua classificação. As estruturas ondulatórias correspondem a ondulaçóes ou trens de onda, enquanto que os sólitons referem-se a estruturas morfológicas usualmente denominadas "kinks" (Tomita et al., 1987). O valor médio do comprimento de onda, corrigido dos efeitos de projeção, Lc medido em 16 estruturas ondulatórias distintas corresponde a (2,2 +- 0,2) x 10^6 km. O valor médio da velocidade de fase cometocêntrica, corrigida dos efeitos de projeção, Vfc é igual a (114 +- 31) km/s e a amplitude média A da onda corresponde a (2,8 +- 0,5) x 10^5 km. Lc e A tendem a aumentar com o incremento da distància cometocêntrica. As ondas são claramente não-lineares e é discutido o local de sua excitação. A distribuição dos D.E.'s na distància heliocêntrica apresenta um caráter bimodal possivelmente associado com a distribuição espacial das fronteiras de setor magnético do meio interplanetário. Em geral, pode-se associar a ocorrência de um "knot" e/ou de uma cauda do tipo Swan com a ocorrência de um D.E., mas este último pode ocorrer independentemente. Os 47 D.E.'s em diferentes fases de evolução foram fotografados em 47 imagens distintas que

  2. PanDA for COMPASS at JINR

    NASA Astrophysics Data System (ADS)

    Petrosyan, A. Sh.

    2016-09-01

    PanDA (Production and Distributed Analysis System) is a workload management system, widely used for data processing at experiments on Large Hadron Collider and others. COMPASS is a high-energy physics experiment at the Super Proton Synchrotron. Data processing for COMPASS runs locally at CERN, on lxbatch, the data itself stored in CASTOR. In 2014 an idea to start running COMPASS production through PanDA arose. Such transformation in experiment's data processing will allow COMPASS community to use not only CERN resources, but also Grid resources worldwide. During the spring and summer of 2015 installation, validation and migration work is being performed at JINR. Details and results of this process are presented in this paper.

  3. The DA{phi}NE-Light Facility

    SciTech Connect

    Burattini, Emilio; Cinque, Gianfelice; Dabagov, Sultan; Grilli, Antonio; Marcelli, Augusto; Pace, Elisabetta; Piccinini, Massimo; Raco, Agostino; Monti, Francesca

    2004-05-12

    The new Synchrotron Radiation facility at Frascati exploits the intense photon emission from DA{phi}NE, the 0,51 GeV storage ring circulating over 1 A of electrons. Among the three beamlines commissioned, the Synchrotron INfrared Beamline At Da{phi}ne (SINBAD) is fully operational by a brilliant SR beam spanning the entire IR. Recently, the soft X-ray beamline has been characterized and, once implemented the double-crystal monocromator, X-ray Absorption Spectroscopy is applied on material standards in the distinguishing energy region below 4 keV. An UltraViolet line, presently dedicated to photobiology dosimetry, has also given first results on cell irradiation in the UVB band.

  4. [Leonardo da Vinci--a dyslectic genius?].

    PubMed

    Røsstad, Anna

    2002-12-10

    Leonardo da Vinci's texts consist almost exclusively of scientific notes. Working on a book on Leonardo's art, I studied all Leonardo's published texts carefully for any new information. In some prefaces I came to suspect that Leonardo might have suffered from dyslexia. This article considers the question of whether it is possible to find indications of dyslexia in Leonardo's texts and in the accounts of his life.

  5. The Real Code of Leonardo da Vinci

    PubMed Central

    Ose, Leiv

    2008-01-01

    Leonardo da Vinci was born in Italy. Among the researchers and scientists, he is favourably known for his remarkable efforts in scientific work. His investigations of atherosclerosis judiciously combine three separate fields of research. In 1506, he finished his masterpiece, painting of Mona Lisa. A careful clinical examination of the famous painting reveals a yellow irregular leather-like spot at the inner end of the left upper eyelid and a soft bumpy well-defined swelling of the dorsum of the right hand beneath the index finger about 3 cm long. This is probably the first case of familial hypercholesterolemia (FH). The FH code of Leonardo da Vinci was given immense consideration by scientists like Carl Muller, who described the xanthomas tuberosum and angina pectoris. On the contrary, Akira Endo searched for microbial metabolites that would inhibit HMG-CoA reductase, the rate-limiting enzyme in the synthesis of cholesterol and finally, Michael Brown and Joseph Goldstein published a remarkable series of elegant and insightful papers in the 70s and 80s. They established that the cellular uptake of low-density lipoprotein (LDL) essentially requires the LDL receptor. In conclusion: this was the real Code of Leonardo da Vinci. PMID:19924278

  6. Leonardo da Vinci's studies of the heart.

    PubMed

    Shoja, Mohammadali M; Agutter, Paul S; Loukas, Marios; Benninger, Brion; Shokouhi, Ghaffar; Namdar, Husain; Ghabili, Kamyar; Khalili, Majid; Tubbs, R Shane

    2013-08-20

    Leonardo da Vinci's detailed drawings are justly celebrated; however, less well known are his accounts of the structures and functions of the organs. In this paper, we focus on his illustrations of the heart, his conjectures about heart and blood vessel function, his experiments on model systems to test those conjectures, and his unprecedented conclusions about the way in which the cardiovascular system operates. In particular, da Vinci seems to have been the first to recognize that the heart is a muscle and that systole is the active phase of the pump. He also seems to have understood the functions of the auricles and pulmonary veins, identified the relationship between the cardiac cycle and the pulse, and explained the hemodynamic mechanism of valve opening and closure. He also described anatomical variations and changes in structure and function that occurred with age. We outline da Vinci's varied career and suggest ways in which his personality, experience, skills and intellectual heritage contributed to these advances in understanding. We also consider his influence on later studies in anatomy and physiology.

  7. Turbocharging the DA465 gasoline engine

    NASA Astrophysics Data System (ADS)

    Zhang, Peng-Qi; Zong, Li-Jun; Wang, Yin-Yan

    2008-06-01

    In order to improve performance of the DA465Q gasoline engine, a substantial amount of research was done to optimize its turbocharging system. The research led to the GT12 turbocharger being selected and its turbocharging parameters being settled. Based on these tests, rational matching was worked out for respective components of the turbocharging system. Results show that this turbocharger allows the engine to easily meet the proposed requirements for power and economic performance, giving insight into further performance improvements for gasoline engines.

  8. Projeto do sistema anti-ressonante da fiação dos transdutores para o detector Mario Schenberg

    NASA Astrophysics Data System (ADS)

    Vieira, S. J.., Jr.; Melo, J. L.

    2003-08-01

    O detector de ondas gravitacionais Mario Schenberg está sendo projetado e construído pelo grupo Gráviton. Sua construção está ocorrendo no Laboratório de Estado Sólido e Baixas Temperaturas (LESBT) da Universidade de São Paulo, na cidade de São Paulo. Esse detector possui uma massa ressonante esférica de cobre-alumínio, com 65 cm de diâmetro, pesando aproximadamente 1150 Kg, suspensa por um sistema de isolamento vibracional, que se encontra em fase de testes preliminares. A real eficácia desse sistema, entretanto, só poderá ser comprovada quando o detector estiver aparelhado com, pelo menos, um transdutor eletromecânico de altíssima sensibilidade acoplado à massa ressonante. Neste momento, não só este sistema de isolamento vibracional será posto em teste, como o do projeto da fiação que transporta os sinais de microondas até os transdutores e destes para a pré-amplificação. Apesar dessa fiação ter sido projetada para não apresentar nenhum contato com a superfície esférica da antena, de maneira a não haver nenhuma transmissão de ruído vibracional do laboratório para esta, deve-se minimizar o ruído microfônico produzido nessa fiação por oscilações mecânicas, uma vez que ela não utiliza nenhum sistema de isolamento vibracional. Com o intuito de resolver este problema, projetamos uma estrutura, formada por pequenos cilindros conectados por barras, a qual não terá nenhuma ressonância mecânica na faixa de freqüências de interesse para detecção (3000 - 3400 Hz). Desta forma, as vibrações nessa faixa não serão amplificadas. O projeto foi feito usando iterativamente, de maneira a otimizar os resultados obtidos, o programa de elementos finitos Msc/Nastran. Através de simulações feitas neste programa, determinamos os parâmetros geométricos ideais a serem utilizados, os quais proporcionam a maior região espectral de interesse livre de ressonâncias.

  9. Revista de Documentacao de Estudos em Linguistica Teorica e Aplicada (DELTA): Novos Estudos em Gamatica Gerativa, 2001 (Journal of Documentary Studies in Theoretical and Applied Linguistics [DELTA]: New Studies in Generative Grammar, 2001).

    ERIC Educational Resources Information Center

    Barbara, Leila, Ed.; Rajagopalan, Kanavillil, Ed.

    2001-01-01

    These two issues of volume 17, include the following articles: "The Competing Motivation Model in the Functional Domains of Negation" (M. Angelica Furtado da Cunha); "Discursive Resonance and Politeness in Reading and Writing Practices" (Silvana Serrani Infante); "The Acquisition of Relative Clauses in Brazilian Portuguese" (Maria Cecilia…

  10. The Case: Bunche-Da Vinci Learning Partnership Academy

    ERIC Educational Resources Information Center

    Eisenberg, Nicole; Winters, Lynn; Alkin, Marvin C.

    2005-01-01

    The Bunche-Da Vinci case described in this article presents a situation at Bunche Elementary School that four theorists were asked to address in their evaluation designs (see EJ791771, EJ719772, EJ791773, and EJ792694). The Bunche-Da Vinci Learning Partnership Academy, an elementary school located between an urban port city and a historically…

  11. 40 CFR 60.52Da - Recordkeeping requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Recordkeeping requirements. 60.52Da Section 60.52Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) STANDARDS OF PERFORMANCE FOR NEW STATIONARY SOURCES Standards of Performance for Electric Utility Steam Generating Units for...

  12. Asteroid 2012 DA14 To Whiz Past Earth Safely

    NASA Video Gallery

    The small near-Earth asteroid 2012 DA14 will pass very close to Earth on Feb. 15, 2013. Asteroid 2012 DA14 will be closest to Earth at about 11:24 a.m. PST (2:24 p.m. EST and 1924 UTC), on Feb. 15,...

  13. DA-6034 Induces [Ca(2+)]i Increase in Epithelial Cells.

    PubMed

    Yang, Yu-Mi; Park, Soonhong; Ji, Hyewon; Kim, Tae-Im; Kim, Eung Kweon; Kang, Kyung Koo; Shin, Dong Min

    2014-04-01

    DA-6034, a eupatilin derivative of flavonoid, has shown potent effects on the protection of gastric mucosa and induced the increases in fluid and glycoprotein secretion in human and rat corneal and conjunctival cells, suggesting that it might be considered as a drug for the treatment of dry eye. However, whether DA-6034 induces Ca(2+) signaling and its underlying mechanism in epithelial cells are not known. In the present study, we investigated the mechanism for actions of DA-6034 in Ca(2+) signaling pathways of the epithelial cells (conjunctival and corneal cells) from human donor eyes and mouse salivary gland epithelial cells. DA-6034 activated Ca(2+)-activated Cl(-) channels (CaCCs) and increased intracellular calcium concentrations ([Ca(2+)]i) in primary cultured human conjunctival cells. DA-6034 also increased [Ca(2+)]i in mouse salivary gland cells and human corneal epithelial cells. [Ca(2+)]i increase of DA-6034 was dependent on the Ca(2+) entry from extracellular and Ca(2+) release from internal Ca(2+) stores. Interestingly, these effects of DA-6034 were related to ryanodine receptors (RyRs) but not phospholipase C/inositol 1,4,5-triphosphate (IP3) pathway and lysosomal Ca(2+) stores. These results suggest that DA-6034 induces Ca(2+) signaling via extracellular Ca(2+) entry and RyRs-sensitive Ca(2+) release from internal Ca(2+) stores in epithelial cells.

  14. Leonardo da Vinci's contributions to neuroscience.

    PubMed

    Pevsner, Jonathan

    2002-04-01

    Leonardo da Vinci (1452-1519) made far-reaching contributions to many areas of science, technology and art. Leonardo's pioneering research into the brain led him to discoveries in neuroanatomy (such as those of the frontal sinus and meningeal vessels) and neurophysiology (he was the first to pith a frog). His injection of hot wax into the brain of an ox provided a cast of the ventricles, and represents the first known use of a solidifying medium to define the shape and size of an internal body structure. Leonardo developed an original, mechanistic model of sensory physiology. He undertook his research with the broad goal of providing physical explanations of how the brain processes visual and other sensory input, and integrates that information via the soul.

  15. Da Costa's syndrome or neurocirculatory asthenia.

    PubMed Central

    Paul, O

    1987-01-01

    The syndrome variously called Da Costa's syndrome, effort syndrome, neurocirculatory asthenia, etc has been studied for more than 100 years by many distinguished physicians. Originally identified in men in wartime, it has been widely recognised as a common chronic condition in both sexes in civilian life. Although the symptoms may seem to appear after infections and various physical and psychological stresses, neurocirculatory asthenia is most often encountered as a familial disorder that is unrelated to these factors, although they may aggravate an existing tendency. Respiratory complaints (including breathlessness, with and without effort, and smothering sensations) are almost universal, and palpitation, chest discomfort, dizziness and faintness, and fatigue are common. The physical examination is normal. The aetiology is obscure but patients usually have a normal life span. Reassurance and measures to improve physical fitness are helpful. PMID:3314950

  16. 75 FR 52292 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Diamond Aircraft... new airworthiness directive (AD) for all Diamond Aircraft Industries GmbH Models DA 40 and DA...

  17. 75 FR 75868 - Airworthiness Directives; Diamond Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-07

    ... Register on August 25, 2010 (75 FR 52292). That NPRM proposed to require a retrofit of the rear passenger... 12866, (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034... Aircraft Industries GmbH Models DA 40 and DA 40F Airplanes AGENCY: Federal Aviation Administration,...

  18. Metal Lines in DA White Dwarfs

    NASA Astrophysics Data System (ADS)

    Zuckerman, B.; Koester, D.; Reid, I. N.; Hünsch, M.

    2003-10-01

    We report Keck telescope HIRES echelle observations of DA white dwarfs in a continuation of an extensive search for metals. These spectra are supplemented with new JHK magnitudes that are used to determine improved atmospheric parameters. Of the DA white dwarfs not in binary or common proper motion systems, about 25% show Ca II lines. For these, Ca abundances are determined from comparison with theoretical equivalent widths from model atmosphere calculations; in a few cases we also obtain Mg, Fe, Si, and Al abundances. If Ca is not observed, we generally determine very stringent upper limits. We compare the data to predictions of previously published models involving the accretion/diffusion of interstellar matter and of comets. The derived abundances are not obviously compatible with the predictions of either model, which up to now could only be tested with traces of metals in helium-rich white dwarfs. By modifying certain assumptions in the published interstellar accretion model we are able to match the distribution of the elements in the white dwarf atmospheres, but, even so, tests of other expectations from this scenario are less successful. Because comet accretion appears unlikely to be the primary cause of the DAZ phenomenon, the data suggest that no more than about 20% of F-type main-sequence stars are accompanied by Oort-like comet clouds. This represents the first observational estimate of this fraction. A plausible alternative to the accretion of cometary or interstellar matter is disruption and accretion of asteroidal material, a model first suggested in 1990 to explain excess near-infrared emission from the DAZ G29-38. An asteroidal debris model to account for the general DAZ phenomenon does not presently disagree with the HIRES data, but neither is there any compelling evidence in support of such a model. The HIRES data indicate that in close red dwarf/white dwarf binaries not known to be cataclysmic variables there is, nonetheless, significant mass

  19. Massa do gás e das estrelas em aglomerados: eficiência da formação estelar

    NASA Astrophysics Data System (ADS)

    Laganá, T. F.; Lima Neto, G. B.

    2003-08-01

    Os aglomerados de galáxias apresentam um interesse especial para a cosmologia observacional. Eles são as maiores estruturas ligadas pela gravitação no Universo e relaxadas na região central. A comparação entre a massa do gás intra-aglomerado (responsável por ~25% da massa total, inferida a partir de observações em raios-X), a massa contida nas estrelas (i.e., nas galáxias) e a massa total (incluindo a matéria escura não bariônica), nos dá informações importantes sobre os processos de formação e evolução de aglomerados. Por exemplo, a razão entre a massa do gás e a massa total é uma medida da fração de bárions no Universo (razão entre a matéria bariônica e matéria escura) e, utilizando a densidade de bárions predita pela nucleosíntese primordial, podemos deduzir a densidade de matéria escura no Universo (cf. White et al. 1993). O objetivo deste trabalho é obter as razões entre as massas do gás, estelar (contida nas galáxias), e a total (massa dinâmica). As massas do gás e total são obtidas a partir das análises fotométrica e espectroscópica em raios-X enquanto que a massa estelar é obtida pela análise fotométrica das galáxias. Esta análise foi aplicada ao aglomerado Abell 496 observado pelo satélite XMM-Newton. A massa contida nas galáxias foi estimada a partir da função de luminosidade obtida por Durret et al. (2002). Para determinar as massas dinâmica e do gás nos precisamos determinar os perfis radiais de densidade e temperatura. Nós apresentaremos aqui estes resultados e suas implicações na eficiência da formação estelar em Abell 496.

  20. Characterization of the isolated 20 kDa and 50 kDa fragments of the myosin head.

    PubMed

    Muhlrad, A; Kasprzak, A A; Ue, K; Ajtai, K; Burghardt, T P

    1986-01-30

    We have isolated two proteolytic fragments of subfragment 1 (S-1) of myosin from rabbit skeletal muscle. These fragments, identified by their molecular weights of 20 and 50 kDa, may be functional domains that, when isolated, retain their specific function. We have studied several structural and functional features of the 20 and 50 kDa fragments. Considerable secondary structure in both fragments has been observed in CD spectrum studies. Previously CD spectra showed 64% ordered structure for the 20 kDa fragment (Muhlrad and Morales, M.F. (1984) Proc. Natl. Acad. Sci. 81, 1003) and here we show 71% ordered structures for the 50 kDa fragment. Fluorescence lifetime studies of tryptophan residues in the 50 kDa fragment and 1,5-IAEDANS-labeled SH-1 in the 20 kDa fragment are used to investigate the tertiary structure of the fragments. We find the tertiary structure relating to this measurement of both fragments to be intact; however, the reaction of 1,5-IAEDANS with SH-1 on the isolated 20 kDa fragment is less specific than with S-1. Furthermore, the fragments showed a tendency to aggregate. The domain concept of S-1 was supported by the characteristic biochemical function of the isolated fragments. Both of the fragments were effective in competing with S-1 for binding to actin in acto-S-1 ATPase measurements. From these studies and in direct binding measurement the 20 kDa fragment proved to bind with higher affinity to actin than did the 50 kDa fragment.

  1. ANÁLISE DA INSERÇÃO DOS TEMAS DE HUMANIDADES E ÉTICA, COM METODOLOGIA DE APRENDIZAGEM BASEADA EM PROBLEMAS, EM CURRICULO MÉDICO INTEGRADO EM ESCOLA PÚBLICA NO DISTRITO FEDERAL, BRASIL

    PubMed Central

    Novaes, Maria Rita Carvalho Garbi; Novaes, Luiz Carlos Garcez; Guilhem, Dirce; Lolas, Fernando; Silveira, Carla; Guiotti, Murilo

    2009-01-01

    Objetivo Realizar uma análise da inserção da ética e humanidades no currículo do Curso de Medicina da Escola Superior em Ciências da Saúde - ESCS, escola pública do Distrito Federal, Brasil, de forma a contribuir com o processo de gestão curricular. Metodologia O Estudo é de coorte e documental. Foram pesquisados 37 termos relacionados à ética e 36 referentes à humanização nos objetivos educacionais e conteúdo dos módulos temáticos, habilidades e atitudes e interação ensino-serviço-comunidade, de 1a a 4a série e no programa do internato no currículo (ano 2006) e no projeto pedagógico do Curso de Medicina (2001). Resultados Maior inserção da humanização, ética e bioética na 1a e 2a série, quando comparado à inserção na 3a e 4a série e no internato, (IC95%-α=0,034, pvalue=0,007). Unidade de habilidades e atitudes: freqüência das 3 temáticas no currículo da 1a a 4a séries (IC95%-α=0,026, pvalue=0,013). Quando comparada a inserção entre o internato e as quatro primeiras séries, observa-se que nestas a inserção da temática humanização é superior (IC95%-α=0,042, pvalue=0,029). Conclusão O currículo desenvolvido no ano de 2006 na ESCS apresentou correlação com o projeto pedagógico do curso e contemplou a temática de forma abrangente, em todas as séries e internato. PMID:20396594

  2. Tree Branching: Leonardo da Vinci's Rule versus Biomechanical Models

    PubMed Central

    Minamino, Ryoko; Tateno, Masaki

    2014-01-01

    This study examined Leonardo da Vinci's rule (i.e., the sum of the cross-sectional area of all tree branches above a branching point at any height is equal to the cross-sectional area of the trunk or the branch immediately below the branching point) using simulations based on two biomechanical models: the uniform stress and elastic similarity models. Model calculations of the daughter/mother ratio (i.e., the ratio of the total cross-sectional area of the daughter branches to the cross-sectional area of the mother branch at the branching point) showed that both biomechanical models agreed with da Vinci's rule when the branching angles of daughter branches and the weights of lateral daughter branches were small; however, the models deviated from da Vinci's rule as the weights and/or the branching angles of lateral daughter branches increased. The calculated values of the two models were largely similar but differed in some ways. Field measurements of Fagus crenata and Abies homolepis also fit this trend, wherein models deviated from da Vinci's rule with increasing relative weights of lateral daughter branches. However, this deviation was small for a branching pattern in nature, where empirical measurements were taken under realistic measurement conditions; thus, da Vinci's rule did not critically contradict the biomechanical models in the case of real branching patterns, though the model calculations described the contradiction between da Vinci's rule and the biomechanical models. The field data for Fagus crenata fit the uniform stress model best, indicating that stress uniformity is the key constraint of branch morphology in Fagus crenata rather than elastic similarity or da Vinci's rule. On the other hand, mechanical constraints are not necessarily significant in the morphology of Abies homolepis branches, depending on the number of daughter branches. Rather, these branches were often in agreement with da Vinci's rule. PMID:24714065

  3. Cloning of the 52-kDa chitinase gene from Serratia marcescens KCTC2172 and its proteolytic cleavage into an active 35-kDa enzyme.

    PubMed

    Gal, S W; Choi, J Y; Kim, C Y; Cheong, Y H; Choi, Y J; Lee, S Y; Bahk, J D; Cho, M J

    1998-03-01

    A chitinase gene (pCHI52) encoding the 52-kDa chitinase was isolated from a Serratia marcescens KCTC2172 cosmid library. This chitinase gene consists of 2526 bp with an open reading frame that encodes 485 amino acids. Escherichia coli harboring the pCHI52 gene secreted not only a 52-kDa but also a 35-kDa chitinase into the culture supernatant. We purified both 52-kDa and 35-kDa chitinases using a chitin affinity column and Sephacryl-S-300 gel filtration chromatography. We determined that the 17 N-terminal amino acid sequences of the 52-kDa and the 35-kDa chitinase are identical. Furthermore, a protease obtained from S. marcescens KCTC2172 cleaved the 52-kDa chitinase into the 35-kDa protein with chitinase activity. These results suggest that the 35-kDa chitinase derives from the 52-kDa chitinase by post-translational proteolytic modification. The optimal reaction temperature of 45 degrees C and the optimal pH of 5.5 were identical for both enzymes. The specific activities of the 52-kDa and 35-kDa chitinases on natural swollen chitin were 67 mumol min-1 mg-1 and 60 mumol min-1 mg-1, respectively.

  4. Translation, Cultural Adaptation and Validation of the Power of Food Scale for Use by Adult Populations in Portugal.

    PubMed

    Ribeiro, Gabriela; Santos, Osvaldo; Camacho, Marta; Torres, Sandra; Mucha-Vieira, Filipa; Sampaio, Daniel; Oliveira-Maia, Albino J

    2015-01-01

    Introdução: A Power of Food Scale é uma escala que avalia um constructo designado por fome hedónica, frequentemente definido como o apetite por alimentos de elevada palatibilidade, mais do que a sua ingestão propriamente dita. Este estudo teve como objetivo a caracterização das propriedades psicométricas de uma versão portuguesa da Power of Food Scale, nomeadamente da sua fiabilidade e validade de constructo. Material e Métodos: Os dados foram obtidos de 1266 participantes adultos, provenientes de três amostras distintas: duas amostras não clínicas e uma amostra clínica de indivíduos com obesidade severa, candidatos a cirurgia da obesidade. Resultados: Verificamos que a estrutura factorial da versão portuguesa da Power of Food Scale é semelhante à do questionário original e que apresenta fiabilidade teste-reteste e validade de constructo adequadas. Adicionalmente, apresentamos neste estudo o primeiro contributo para a definição de normas populacionais para os scores da Power of Food Scale. Discussão: A versão portuguesa da Power of Food Scale apresentou boas propriedades psicométricas: é um instrumento válido e fiável para a avaliação do constructo fome hedónica, suportando a sua utilização tanto em amostras clínicas como não clínicas, bem como em estudos longitudinais. Conclusão: Estes resultados demostram que a versão portuguesa da Power of Food Scale é um instrumento útil, tanto em contexto clínico, como de investigação, nomeadamente para o estudo do constructo fome hedónica em populações de língua portuguesa, incluindo populações clínicas de indivíduos com obesidade.

  5. Determinação do extremo de baixa luminosidade da função de luminosidade através de simulações de Monte Carlo

    NASA Astrophysics Data System (ADS)

    Campos, P. E.; Mendes de Oliveira, C.

    2003-08-01

    Neste trabalho apresentamos os principais resultados do estudo da população de galáxias anãs de baixo brilho superficial dos grupos compactos HCG44 e HCG68. A inclinação do extremo de baixa luminosidade a da função de luminosidade desses grupos foi estimado através de simulações de Monte Carlo. O método consistiu em simular galáxias anãs de baixo brilho superficial em imagens reais seguindo uma distribuição de magnitudes dada uma função de Schechter. Foram testados vários modelos com diferentes valores para os parâmetros M* e a da função de Schechter, e para cada um dos modelos foi repetido o mesmo processo de recuperação, seleção e análise usados com as galáxias anãs dos grupos. Os resultados de cada modelo foram então comparados com o observado nos grupos. Nossos resultados indicam que a distribuição de magnitudes (para magnitudes absolutas até MR»-12) observadas nos grupos são compatíveis com uma função de luminosidade de Schechter dada por um M* = -16 e um a entre -1.0 e -1.4, e portanto de acordo com a tendência observada de uma inclinação do extremo de baixa luminosidade da função de luminosidade de grupos mais plana (~1.2, Trentham & Tully 2002, MNRAS, 335, 712) que esperado pelo modelo hierárquico de formação de estruturas CDM (Cold Dark Matter - Matéria Escura Fria).

  6. PKC phosphorylates residues in the N-terminal of the DA transporter to regulate amphetamine-induced DA efflux.

    PubMed

    Wang, Qiang; Bubula, Nancy; Brown, Jason; Wang, Yunliang; Kondev, Veronika; Vezina, Paul

    2016-05-27

    The DA transporter (DAT), a phosphoprotein, controls extracellular dopamine (DA) levels in the central nervous system through transport or reverse transport (efflux). Multiple lines of evidence support the claim that PKC significantly contributes to amphetamine-induced DA efflux. Other signaling pathways, involving CaMKII and ERK, have also been shown to regulate DAT mediated efflux. Here we assessed the contribution of putative PKC residues (S4, S7, S13) in the N-terminal of the DAT to amphetamine-induced DA efflux by transfecting DATs containing different serine to alanine (S-A) point mutations into DA pre-loaded HEK-293 cells and incubating these cells in amphetamine (2μM). The effects of a S-A mutation at the non-PKC residue S12 and a threonine to alanine (T-A) mutation at the ERK T53 residue were also assessed for comparison. WT-DATs were used as controls. In an initial experiment, we confirmed that inhibiting PKC with Go6976 (130nM) significantly reduced amphetamine-induced DA efflux. In subsequent experiments, cells transfected with the S4A, S12A, S13A, T53A and S4,7,13A mutants showed a reduction in amphetamine-induced DA efflux similar to that observed with Go6976. Interestingly, cells transfected with the S7A mutant, identified by some as a PKC-PKA residue, showed unperturbed WT-DAT levels of amphetamine-induced DA efflux. These results indicate that phosphorylation by PKC of select residues in the DAT N-terminal can regulate amphetamine-induced efflux. PKC can act either independently or in concert with other kinases such as ERK to produce this effect.

  7. A Concepção de Universo entre Alunos do Ensino Médio de São Paulo e suas Fontes de Aquisição

    NASA Astrophysics Data System (ADS)

    Araújo, M. A. A.; Elias, D. C. N.; Amaral, L. H.; Araújo, M. S. T.; Voelzke, M. R.

    2006-08-01

    Nesse trabalho procurou-se identificar por meio de um questionário as concepções de Universo, de espaço e tempo que sustentam a visão de mundo de um grupo de 270 estudantes de Ensino Médio, pertencentes a três escolas de São Paulo. As questões relacionadas aos conhecimentos prévios dos estudantes permitiram constatar que há pouco conhecimento acerca dos temas investigados, destacando-se que apenas 20% dos alunos foram capazes de relacionar as semanas com as fases da lua, enquanto 28% associaram as estações do ano à inclinação do eixo de rotação da Terra e 23% tinham noções das distâncias entre objetos celestes próximos da Terra. Enquanto 56% conseguiram relacionar o Big Bang com a origem do Universo, verificou-se que 37% reconheciam ano-luz como unidade de distância e 60% concebiam o Sol como uma estrela. No que se refere às fontes de aquisição que proporcionaram esses conhecimentos, apesar de 60% dos alunos indicarem a escola como principal fonte dos conhecimentos de Ast! ronomia, verificou-se claramente que para a maioria dos alunos seus conceitos ainda são inadequados, havendo necessidade de aprimoramento da abordagem desses conteúdos, pois apesar de popular, a Astronomia ainda é veiculada de maneira pouco esclarecedora e com imprecisões. Nesse contexto, são discutidas algumas possíveis contribuições que podem ser dadas para o ensino de Astronomia pelo uso das ferramentas computacionais nas escolas.

  8. The Moon Phases in a Paper Box. (Spanish Title: Las Fases de la Luna en Una Caja de Cartón.) As Fases da Lua Numa Caixa de Papelão

    NASA Astrophysics Data System (ADS)

    de Fátima O. Saraiva, Maria; Amador, Cláudio B.; Kemper, Érico; Goulart, Paulo; Muller, Angela

    2007-12-01

    material didático de baixo custo para demonstração do conceito de fases de um corpo iluminado. O principal objetivo de nosso material é facilitar a compreensão das fases da Lua da perspectiva de um observador na Terra. O material ajuda na visualização de dois efeitos importantes: (1º) mesmo tendo sempre a metade da "Lua" (representada por uma bolinha de isopor ou de ping-pong) iluminada pelo "Sol" (representado por uma fonte de luz natural ou artificial), nós vemos diferentes frações de sua superfície iluminada, dependendo do ângulo pelo qual a olhamos; (2º) a orientação da borda convexa da Lua nas fases Crescente e Minguante também depende da perspectiva pela qual a olhamos da Terra. O uso de uma caixa fechada permite observar o contraste entre as diferentes fases sem necessidade de estar em uma sala escurecida. Apresentamos também um texto explicativo sobre fases da Lua, enfatizando a dependência da aparência da parte iluminada com o ângulo de visada.

  9. [Normative Database of Optical Coherence Tomography Parameters in Childhood].

    PubMed

    Queirós, Tatiana; Freitas, Cristina; Guimarães, Sandra

    2015-01-01

    Introdução: A tomografia de coerência óptica é um exame que permite obter imagens de alta resolução dos tecidos in vivo, possibilitando a medição das estruturas oculares, nomeadamente a camada de fibras nervosas da retina e a espessura macular. Como método não invasivo torna-se particularmente útil em crianças, contudo a sua aplicabilidade está limitada pela existência de valores normativos apenas para adultos.Objetivo: Estabelecer na idade pediátrica valores normativos para a espessura da camada de fibras nervosas da retina e espessura macular, averiguando a sua influência com o género, idade, refração, lateralidade e dominância ocular.Material e Métodos: Foram submetidas a exame oftalmológico e a Cirrus HD-tomografia de coerência óptica (Carl Zeiss Meditec) 153 crianças dos quatro aos 17 anos.Resultados: Obtiveram-se valores da espessura média global da camada de fibras nervosas da retina de 97,90 μm. Não se detectaram diferenças entre géneros e com a idade, mas sim consoante a lateralidade e dominância ocular. Verificou-se um aumento da espessura com refrações positivas. Com o protocolo Macular Cube 512 x 128 verificou-se que o campo central apresentou a menor espessura (250,35 μm), apresentando os rapazes maior espessura macular.Discussão: Os valores da espessura da camada de fibras nervosas da retina e da espessura macular obtidos são comparáveis a estudos recentes. A distribuição da espessura por quadrantes respeita a distribuição normal da camada de fibras nervosas da retina. A espessura macular revelou-se superior no género masculino (campo central e anel interno), dados estes também concordantes com estudos prévios.Conclusão: Estabelecemos as normativas da espessura da camada de fibras nervosas da retina e espessura macular em crianças portuguesas saudáveis, dados estes que reestruturam a avaliação e interpretação dos parâmetros obtidos pela tomografia de coerência óptica no diagnóstico de

  10. Tendências de teses e dissertações sobre ensino de astronomia no Brasil

    NASA Astrophysics Data System (ADS)

    Bretones, P. S.; Megid Neto, J.

    2003-08-01

    Neste trabalho são apresentados os resultados de uma pesquisa do tipo estado da arte sobre teses e dissertações defendidas no Brasil e relativas ao ensino de Astronomia. Teve por objetivo identificar essa produção e conhecer as principais tendências da pesquisa nesse campo. O procedimento inicial consistiu de um levantamento bibliográfico junto ao Centro de Documentação em Ensino de Ciências (CEDOC) da Faculdade de Educação da UNICAMP e ao Banco de Teses da CAPES disponível na Internet. Foram localizadas 13 dissertações de mestrado e 3 teses de doutorado, as quais foram estudadas em função dos seguintes aspectos: instituição, ano de defesa, nível escolar abrangido no estudo, foco temático do estudo e gênero de trabalho acadêmico. Deste conjunto de pesquisas, 13 (81,3%) delas foram defendidas a partir da segunda metade dos anos 90, indicando uma preocupação mais recente com temas relativos ao ensino de Astronomia no conjunto da produção acadêmica em programas de pós-graduação no Brasil. Verificou-se que 43,7% dos trabalhos foram produzidas na USP e 18,8% na UNICAMP. Quanto ao nível escolar abrangido nos estudos, predominaram os estudos direcionados ao Ensino Fundamental de 5a a 8a séries (62,5%). No que diz respeito ao foco temático das pesquisas, as principais tendências voltaram-se: 56,3% para Conteúdo e Método; 43,8% para Concepções do Professor; 37,5% para Currículo e Programas; 37,5% para Recursos Didáticos. Quanto ao gênero de trabalho acadêmico, verificou-se que 43,8% são de Pesquisa Experimental e 31,3% de Pesquisa de Análise de Conteúdo. Estudos de revisão bibliográfica como este visam colaborar com a divulgação ampla da produção acadêmica em determinada área, traçando algumas de suas tendências. Ao mesmo tempo possibilita, a partir de investigações decorrentes, apontar as suas contribuições para o ensino e sinalizar com necessidades a serem supridas por futuras pesquisas.

  11. Geomagnetic observations on tristan da cunha, south atlantic ocean

    USGS Publications Warehouse

    Matzka, J.; Olsen, N.; Maule, C.F.; Pedersen, L.W.; Berarducci, A.M.; Macmillan, S.

    2009-01-01

    Few geomagnetic ground observations exist of the Earth's strongest core field anomaly, the South Atlantic Anomaly (SAA). The geomagnetic repeat station on the island Tristan da Cunha, located half-way between South Africa and South America at 37?? 05' S, 12?? 18' W, is therefore of crucial importance. We have conducted several sets of repeat station measurements during magnetically quiet conditions (Kp 2o or less) in 2004. The procedures are described and the results are compared to those from earlier campaigns and to the predictions of various global field models. Features of the local crustal bias field and the solar quiet daily variation are discussed. We also evaluate the benefit of continuous magnetic field recordings from Tristan da Cunha, and argue that such a data set is a very valuable addition to geomagnetic satellite data. Recently, funds were set up to establish and operate a magnetometer station on Tristan da Cunha during the Swarm magnetic satellite mission (2011-2014).

  12. 1991 DA: An asteroid in a bizarre orbit

    NASA Technical Reports Server (NTRS)

    Steel, Duncan; Mcnaught, Robert H.; Asher, David

    1992-01-01

    Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that 1991 DA is an extinct or dormant comet. Previous CCD imaging has shown no indication of a coma; spectroscopic observations of 1991 DA which lack any evidence of strong comet-like emissions are reported. Numerical integrations of the orbit of this object were performed which show that is has been remarkably stable for the past approximately 20,000 yr, but chaotic before that. This may allow a new estimate to be made of the physical lifetimes of comets.

  13. The future of PanDA in ATLAS distributed computing

    NASA Astrophysics Data System (ADS)

    De, K.; Klimentov, A.; Maeno, T.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.

    2015-12-01

    Experiments at the Large Hadron Collider (LHC) face unprecedented computing challenges. Heterogeneous resources are distributed worldwide at hundreds of sites, thousands of physicists analyse the data remotely, the volume of processed data is beyond the exabyte scale, while data processing requires more than a few billion hours of computing usage per year. The PanDA (Production and Distributed Analysis) system was developed to meet the scale and complexity of LHC distributed computing for the ATLAS experiment. In the process, the old batch job paradigm of locally managed computing in HEP was discarded in favour of a far more automated, flexible and scalable model. The success of PanDA in ATLAS is leading to widespread adoption and testing by other experiments. PanDA is the first exascale workload management system in HEP, already operating at more than a million computing jobs per day, and processing over an exabyte of data in 2013. There are many new challenges that PanDA will face in the near future, in addition to new challenges of scale, heterogeneity and increasing user base. PanDA will need to handle rapidly changing computing infrastructure, will require factorization of code for easier deployment, will need to incorporate additional information sources including network metrics in decision making, be able to control network circuits, handle dynamically sized workload processing, provide improved visualization, and face many other challenges. In this talk we will focus on the new features, planned or recently implemented, that are relevant to the next decade of distributed computing workload management using PanDA.

  14. Situação da Mulher na Astronomia Brasileira

    NASA Astrophysics Data System (ADS)

    Silva, Adriana V. R.

    2007-07-01

    O conteúdo desse texto surgiu de uma apresentação de mesmo título que fiz na XXXI Reunião Anual da Sociedade Astronômica Brasileira (SAB) em 2005. Esse tema foi inspirado originalmente pela minha participação no "2nd UIPAP International Conference on Women in Physics" realizado entre 23 e 25 de maio de 2005 no Rio de Janeiro. Essa é uma conferência internacional que acontece de três em três anos, sendo que a primeira ocorreu em 2002 na cidade de Paris, França. Participei dessa conferência como membro da delegação da Sociedade Brasileira de Física e um dos trabalhos que apresentei versava sobre a situação das mulheres na Astronomia brasileira, cujos resultados principais discorro a seguir. A situação das astrônomas, baseada nos dados dos sócios da SAB coletados no final de 2004, é comparada com a das físicas brasileiras e também com as nossas colegas americanas. Os dados identificam ainda uma maior evasão da carreira por parte das mulheres do que os homens. Alguns dos possíveis motivos da evasão são discutidos, como o desejo de constituir família e/ou isolamento. Resultados um tanto preocupantes com relação à distribuição de bolsas de produtividade do CNPq também são apresentados. As principais discussões e estratégias recomendadas nesse congresso são mencionadas de forma resumida ao final.

  15. [Educational status and patterns of weight gain in adulthood in Brazil: Estudo Pró-Saúde].

    PubMed

    Fonseca, Maria de Jesus Mendes da; França, Rosana de Figueiredo; Faerstein, Eduardo; Werneck, Guilherme Loureiro; Chor, Dóra

    2012-11-01

    The aim of the present study was to investigate the association between participant and parental educational status (considered as an indicator of socioeconomic status) and participant pattern of weight gain in adulthood. We analyzed data from 2 582 baseline participants (1999) of Estudo Pró-Saúde (Pro-Health Study), a longitudinal investigation of civil servants from a public university in Rio de Janeiro, Brazil. Self-administered questionnaires were used to identify patterns of weight gain in adulthood. Odds ratios (OR) and 95% confidence intervals (95%CI) were estimated for the association between parental and participant educational status and steady weight gain or weight cycling, with stable weight as a reference, using multinomial logistic regression models. For males, lower paternal educational level entailed a chance about 55% lower of weight cycling as compared to stable weight (OR = 0.45; IC95% = 0.26-0.78), whereas lower maternal schooling was related to increased risk of weight cycling, although without reaching statistical significance (OR = 1.68; IC95% = 0.94-3.00). The association between participant educational status and weight history was not statistically significant among men. In women, lower educational status entailed a chance 94% higher of self-reported weight cycling (OR = 1.94; 95% CI = 1.17-3.23), and there was no association between parental educational level and history of weight gain. In this study, changes in weight throughout life, both steady and cyclic, were associated with parental and participant educational status, with major differences between genders.

  16. Publishing Planetary Data to the VO using DaCHS

    NASA Astrophysics Data System (ADS)

    Demleitner, M.

    2014-04-01

    Publishing to the Virtual Observatory has many advantages, but between standards compliance, registration, data organisation, and server operation it is also requires a nontrivial effort if starting from scratch. The DaCHS software from the German Astrophysical Virtual Observatory offers a packaged solution letting publishers concentrate on working with their own data and metadata as much as possible. For the planetary community, publishing is further facilitated by built-in support for the EPN-TAP data model. This contribution will show a typical publishing workflow together with a brief overview of how DaCHS meshes in with the VO and Web-based data usage.

  17. The DaCHS Multi-protocol VO Server

    NASA Astrophysics Data System (ADS)

    Demleitner, M.

    2014-05-01

    GAVO's Data Center Helper Suite (DaCHS) is a suite of tools for publishing data to the Virtual Observatory. It implements all major VO protocols (SCS, SIAP, SSAP, TAP, OAI-PMH). The integrated management and ingestion component allow defining metadata, structure, and services once and re-use the definition throughout the publication cycle from initial metadata aquisition to registry record generation. It has been driving GAVO's data center since 2008 and is now deployed in multiple locations around the globe. This poster briefly describes the design of the system as well as a bird's-eye view of data publishing with DaCHS.

  18. 40 CFR 60.48Da - Compliance provisions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...)), by the hourly heat input rate (measured according to the provisions of § 60.49Da(n)), and dividing... shall conduct the performance test required under § 60.8 using the appropriate methods in appendix A of... second sampling site shall be located at the outlet from the heat recovery steam generating...

  19. 40 CFR 60.48Da - Compliance provisions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... emission rate in lb/MMBtu (measured by the CEMS required under § 60.49Da(c) and (d)), by the hourly heat... section may be used: (1) The owner or operator of an affected duct burner shall conduct the performance... located at the outlet from the heat recovery steam generating unit. Measurements shall be taken at...

  20. 40 CFR 60.48Da - Compliance provisions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...)), by the hourly heat input rate (measured according to the provisions of § 60.49Da(n)), and dividing... shall conduct the performance test required under § 60.8 using the appropriate methods in appendix A of... second sampling site shall be located at the outlet from the heat recovery steam generating...

  1. Leonardo da Vinci and the sinuses of Valsalva.

    PubMed

    Robicsek, F

    1991-08-01

    Recent studies indicate that eddy currents generated by the sinuses of Valsalva play an important role in the physiologic closure of the aortic valve. This process is briefly discussed and evidence is presented that this fact was well known and elaborated upon by the renaissance artist Leonardo da Vinci. This fact is illustrated with his words and drawings.

  2. Studying and Working Abroad. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles recent successful examples of students studying and working abroad as part of the European Commission's Leonardo da Vinci program, which is designed to give students across the European Union the opportunity to experience vocational training in a foreign country. The following examples are presented: (1) 3 Finnish students…

  3. 40 CFR 60.47Da - Commercial demonstration permit.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Steam Generating Units for Which Construction is Commenced After September 18, 1978 § 60.47Da Commercial... potential combustion concentration (80 percent reduction) for each 24-hour period of steam generator... owner or operator of a fluidized bed combustion electric utility steam generator (atmospheric...

  4. Infrared applications for portable devices using IrDA transceivers

    NASA Astrophysics Data System (ADS)

    Krishnan, Sanjay M.; Pamidighantam, Ramana V.; Tan, Wee S.

    2001-11-01

    The internet has been the mantra in last decade and digital convergence is the latest talk on the tech streets. Features that once clearly discerned a mobile phone from a PDA or a PDA from a laptop are being replaced with multi-functional overlapping features and there is a growing need for wireless access and interoperability. Infrared communications has been addressing these needs for quite some time. The technology has matured through standardization efforts of IrDA (Infrared Data Association) and is widely used in PDAs, mobile phones, laptops, printers and digital cameras to name a few. This paper explores some new application scenarios for an IrDA transceiver. The first part of the paper describes a reference architecture for using infrared wireless communication for MP3 file exchange and the various possible user models. The second part of the paper discusses various configurations for implementing an ambient light detection scheme using IrDA transceivers, to turn off the backlighting of a LCD panel of a PDA or mobile phone in the presence of ambient light. The third part of the paper discusses various techniques of enabling extended link distance operation of IrDA transceivers for toy and remote control applications.

  5. Training and Health. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles programs in the fields of health and medicine that are offered through the European Commission's Leonardo da Vinci program. The following programs are profiled: (1) CYTOTRAIN (a transnational vocational training program in cervical cancer screening); (2) Apollo (a program of open and distance learning for paramedical…

  6. Women and Technical Professions. Leonardo da Vinci Series: Good Practices.

    ERIC Educational Resources Information Center

    Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.

    This document profiles programs for women in technical professions that are offered through the European Commission's Leonardo da Vinci program. The following programs are profiled: (1) Artemis and Diana (vocational guidance programs to help direct girls toward technology-related careers); (2) CEEWIT (an Internet-based information and…

  7. 32 CFR 643.121 - Private organizations on DA installations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....121 Section 643.121 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY... DA installations. (a) AR 210-1 defines and classifies private organizations, such as thrift shops and child-care centers, located on Army installations and provides policy guidance for their...

  8. 32 CFR 643.121 - Private organizations on DA installations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....121 Section 643.121 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY... DA installations. (a) AR 210-1 defines and classifies private organizations, such as thrift shops and child-care centers, located on Army installations and provides policy guidance for their...

  9. 32 CFR 643.121 - Private organizations on DA installations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....121 Section 643.121 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY... DA installations. (a) AR 210-1 defines and classifies private organizations, such as thrift shops and child-care centers, located on Army installations and provides policy guidance for their...

  10. 32 CFR 643.121 - Private organizations on DA installations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....121 Section 643.121 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY... DA installations. (a) AR 210-1 defines and classifies private organizations, such as thrift shops and child-care centers, located on Army installations and provides policy guidance for their...

  11. The Potential da Vinci in All of Us

    ERIC Educational Resources Information Center

    Petto, Sarah; Petto, Andrew

    2009-01-01

    The study of the human form is fundamental to both science and art curricula. For vertebrates, perhaps no feature is more important than the skeleton to determine observable form and function. As Leonard da Vinci's famous Proportions of the Human Figure (Virtruvian Man) illustrates, the size, shape, and proportions of the human body are defined by…

  12. 40 CFR 60.48Da - Compliance provisions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... combined cycle systems, either of the procedures described in paragraph (j)(1) or (2) of this section may... carbon dioxide (CO2)) and meet the requirements of § 60.49Da. Alternatively, data from a NOX emission... shall constitute the NOX emission rate from the duct burner of the combined cycle system. (k)...

  13. Da Que Hablar (Something To Talk About), 1991-1993.

    ERIC Educational Resources Information Center

    Da Que Hablar, 1993

    1993-01-01

    This document consists of all 14 issues of a bimonthly serial, from its inception in May 1991 through November 1993. "Da Que Hablar" provides numerous authentic materials from magazines and newspapers to stimulate discussion in Spanish in the foreign language classroom. The articles cover topics such as current events, cultural issues, sports,…

  14. 40 CFR 60.45Da - Standard for mercury (Hg).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Standard for mercury (Hg). 60.45Da... for mercury (Hg). (a) For each coal-fired electric utility steam generating unit other than an IGCC... gases that contain mercury (Hg) emissions in excess of each Hg emissions limit in paragraphs...

  15. Roles and Delegation of Authority (R/DA) System

    SciTech Connect

    ABBOTT,JOHN P.; HUTCHINS,JAMES C.; SCHOCH,DAVID G.

    1999-11-01

    The processes of defining managerial roles and providing for delegation of authority are essential to any enterprise. At most large organizations, these processes are defined in policy manuals and through sets of standard operating procedures for many, if not all, business and administrative functions. Many of these staff-initiated, administrative functions require the routing of documents for approval to one or more levels of management. These employee-oriented, back office types of workflows tend to require more flexibility in determining to whom these documents should go to, while, at the same time, providing the responsible parties with the flexibility to delegate their approval authority or allow others to review their work. Although this practice is commonplace in manual, paper-based processes that exist in many organizations, it is difficult to provide the same flexibility in the more structured, electronic-based, workflow systems. The purpose of this report is to present a framework or architecture for creating a R/DA system and provide some insights associated with its design and utilization. To improve understanding and clarify subsequent discussion, the goals and requirements for the major R/DA system components, namely, the database and interface modules, are initially presented along with the identification of important concepts and the definition of critical terms. Next high-level functions relating the types of inputs to the outputs of the R/DA interface module are introduced and discussed. Then the relationships between the major R/DA modules and the primary components associated with its creation and maintenance are presented and analyzed. Finally, some conclusions are drawn relative to the advantages associated with developing a R/DA system for use in implementing an enterprise-wide, work-facilitating information system.

  16. 32 CFR 635.23 - DA Form 4833 (Commander's Report of Disciplinary or Administrative Action).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DA Form 4833 within 45 days of receipt. (1) Appropriate blocks will be checked and blanks annotated... the disposition on DA Form 4833 as appropriate. That portion of the signature block of DA Form 4833... the format and at the frequency specified by the supported commander....

  17. In vivo breakdown products of the 32 kDa thylakoid herbicide binding protein. [Spirodela oligorrhiza

    SciTech Connect

    Greenberg, B.M.; Mattoo, A.K.; Gaba, V.; Edelman, M.

    1986-04-01

    The 32 kDa herbicide binding protein of PSII is degraded rapidly in the light. This phenomenon was investigated in Spirodela oligorrhiza. When fronds were radiolabeled in the presence of cycloheximide only the chloroplast-encoded proteins were labeled. Under these conditions, a breakdown product of 23.5 kDa was observed. This polypeptide was further degraded with kinetics similar to that of the 32 kDa protein. The 23.5 kDa polypeptide cross-reacted with an antibody specific to the 32 kDa protein. By protease digestion it was determined that the 23.5 kDa polypeptide is the intact N-terminal piece of the 32 kDa protein. Thus, this product corresponds to the membrane anchor of the 32 kDa protein. Using the same antibody, breakdown products of 16 kDa, 14 kDa and 12 kDa were observed. These products have also been observed in Zea mays and Solanum nigrum. Using DCMU during pulse-chase experiments at different light intensities, evidence was obtained that the 23.5 kDa breakdown product is generated in vivo.

  18. Measuring emotional awareness from a cognitive-developmental perspective: Portuguese adaptation studies of the levels of emotional awareness scale.

    PubMed

    Torrado, Marco; Ouakinin, Sílvia; Lane, Richard

    2013-01-01

    Introdução: A Escala de Níveis de Consciência Emocional (LEAS) foi desenvolvida para avaliar o constructo consciência emocional, numa perspectiva cognitivo-desenvolvimentista e influenciada pelas Teorias de Piaget e Werner. É composta por 20 situações ou cenários que evocam emoções e tem sido usada em múltiplas investigações nos domínios da regulação emocional, alexitimia e perturbações psiquiátricas. Trata-se de uma medida bem documentada, validada e precisa. Em virtude da sua extensão, alguns investigadores têm vindo a utilizar uma das formas paralelas que compõe a versão completa, a LEAS-A, sendo contudo notória a vacuidade de estudos que descrevam as qualidades psicométricas desta versão. Face à inexistência de uma medida de caracterização da organização da experiência emocional para a população portuguesa, desenvolveu-se a versão portuguesa da LEAS, caracterizaram-se diversos indicadores de precisão e validade, assim como para a versão reduzida LEAS-A. Materiais e Métodos: Foram desenvolvidos três estudos com estas versões, dois deles com recurso a estudantes universitários e um outro com uma amostra da população em geral. Resultados: A versão portuguesa demonstrou níveis elevados de precisão, mais robustos do que os encontrados em estudos de adaptação da escala noutros países. A LEAS-A apresentou bons níveis de precisão e indicadores de validade discriminante e concorrente. As pontuações obtidas na LEAS-A mostraram-se independentes da presença de afecto negativo e associaram-se significativamente a um estilo cognitivo externalizado, próprio do funcionamento alexitímico. Conclusões: As versões portuguesas da LEAS e da LEAS-A apresentam qualidades psicométricas muito adequadas, o que permite o seu uso científico. São discutidas as implicações da sua utilização nos contextos clínico e de investigação.

  19. The Science Teachers and Their way of Thinking about Astronomy. (Spanish Title: Los Profesores de Ciencias y Sus Formas de Pensar la Astronomía.) Os Professores de Ciências e Suas Formas de Pensar a Astronomia

    NASA Astrophysics Data System (ADS)

    Leite, Cristina; Hosoume, Yassuko

    2007-12-01

    ómicos y la estructura espacial del montaje del Universo son características fundamentales. Muchos indican Sol y estrellas como cosas distintas: el Sol es un objeto caliente y las estrellas son frías. Esos resultados nos preocupan y ciertamente señalan una urgencia de programas de formación continuada para profesores en el contenido de astronomía, pues los PCN's (Parámetros Curriculares Nacionales) recomiendan con énfasis la enseñanza de este contenido. O modo de pensar dos professores de Ciências do ensino fundamental sobre os elementos da Astronomia é o objeto de pesquisa presente neste artigo, cuja metodologia consiste em entrevistas semiestruturadas, filmadas em vídeo e centradas numa perspectiva tridimensional dos elementos astronômicos, possibilitando um mapeamento das concepções das formas e das dimensões da Terra, do Sol, da Lua, dos planetas e das estrelas, bem como da concepção de céu e de Universo como um todo. Os resultados indicam um Universo contendo: Sol, estrelas, planetas e Lua, onde o Sistema Solar é parte do todo. Algumas vezes o Universo é concebido como o próprio Sistema Solar. Os objetos estão no céu ou no Universo o qual, para muitos, se restringe ao espaço que está acima da Terra. A forma plana dos objetos astronômicos e a estrutura espacial da montagem do Universo são características marcantes. Muitos indicam Sol e estrelas como coisas diferentes: Sol é um objeto quente e as estrelas são frias. Esses resultados nos preocupam e certamente sinalizam uma urgência de programas de formação continuada para professores no conteúdo de astronomia, uma vez que os PCN's indicam fortemente o ensino desse conteúdo.

  20. PanDaTox: a tool for accelerated metabolic engineering

    SciTech Connect

    Amitai, Gil; Sorek, Rotem

    2012-04-18

    Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox (www.weizmann.ac.il/pandatox), a web-based resource that provides experimental toxicity information for more than 1.5 million genes from hundreds of different microbial genomes. The toxicity predictions, which were extensively experimentally verified, are based on serial cloning of genes into E. coli as part of the Sanger whole genome shotgun sequencing process. PanDaTox can accelerate metabolic engineering projects by allowing researchers to exclude toxic genes from the engineering plan and verify the clonability of selected genes before the actual metabolic engineering experiments are conducted.

  1. [Lobectomy for lung cancer using the Da Vinci surgical system].

    PubMed

    Nakamura, Hiroshige

    2014-05-01

    Robot-assisted surgery using the da Vinci surgical system has attracted attention because of excellent operability without shaking by joint forceps under the clear vision of a three-dimensional high-definition camera in lung cancer surgery. Although this form of advanced medical care is not yet approved for insurance coverage, it is at the stage of clinical research and expected to be useful in hilar exposure, lymph node dissection, and suturing of the lung parenchyma or bronchus. Lung cancer surgery with the da Vinci system has the advantage of combining thoracotomy and minimally invasive surgery in video-assisted thoracic surgery. However, safety management, education, and significant cost are problems to be resolved. Several important issues such as sharing knowledge and technology of robotic surgery, education, training, development of new instruments, and acquisition of advanced medical insurance are discussed for the future development of robotic surgical systems. PMID:24946522

  2. A novel 29-kDa chicken heat shock protein.

    PubMed

    Einat, M F; Haberfeld, A; Shamay, A; Horev, G; Hurwitz, S; Yahav, S

    1996-12-01

    The family of small heat shock proteins is the more variable among the highly conserved superfamily of heat shock proteins (HSP). Using a metabolic labeling procedure with tissue explants, we have detected in chickens a new member of the small HSP family with an apparent molecular weight of 29-kDa. This protein was induced in broiler chickens' heart muscle and lungs following an in vivo heat stress. The 29-kDa band appears after 3 h of heat stress, much later than the induction of HSP 90, HSP 70, and HSP 27. The late onset of induction suggests that HSP 29 plays a more specific role of a "second stage defense protein". PMID:9000279

  3. The Da Vinci code dynamically de-coded.

    PubMed

    Cohen, Mariam

    2005-01-01

    The novel The Da Vinci Code, by Dan Brown has been on best-seller lists for over two years. An examination of Brown's previous novels reveals a well-designed plot line shared by all four novels that not only makes them good "thrillers" but also creates a mythological structure to the novels that draws on common unconscious fantasies in the same way that fairy tales do. One aspect of this mythological structure is the use of evil conspiracies (and benign ones as well) for the protagonist to overcome. In addition, The Da Vinci Code presents a religious theme involving legends about Mary Magdalene. This theme touches on the role of a feminine aspect to divinity in allowing for an erotic connection with the divine.

  4. TA-DA: A Tool for Astrophysical Data Analysis

    NASA Astrophysics Data System (ADS)

    Da Rio, Nicola; Robberto, Massimo

    2012-12-01

    We present the Tool for Astrophysical Data Analysis (TA-DA), a new software aimed to greatly simplify and improve the analysis of stellar photometric data in comparison with theoretical models, and allow the derivation of stellar parameters from multi-band photometry. Its flexibility allows one to address a number of such problems: from the interpolation of stellar models, or sets of stellar physical parameters in general, to the computation of synthetic photometry in arbitrary filters or units; from the analysis of observed color-magnitude diagrams to a Bayesian derivation of stellar parameters (and extinction) based on multi-band data. TA-DA is available as a pre-compiled Interactive Data Language widget-based application; its graphical user interface makes it considerably user-friendly. In this paper, we describe the software and its functionalities.

  5. TA-DA: A TOOL FOR ASTROPHYSICAL DATA ANALYSIS

    SciTech Connect

    Da Rio, Nicola; Robberto, Massimo

    2012-12-01

    We present the Tool for Astrophysical Data Analysis (TA-DA), a new software aimed to greatly simplify and improve the analysis of stellar photometric data in comparison with theoretical models, and allow the derivation of stellar parameters from multi-band photometry. Its flexibility allows one to address a number of such problems: from the interpolation of stellar models, or sets of stellar physical parameters in general, to the computation of synthetic photometry in arbitrary filters or units; from the analysis of observed color-magnitude diagrams to a Bayesian derivation of stellar parameters (and extinction) based on multi-band data. TA-DA is available as a pre-compiled Interactive Data Language widget-based application; its graphical user interface makes it considerably user-friendly. In this paper, we describe the software and its functionalities.

  6. [Lobectomy for lung cancer using the Da Vinci surgical system].

    PubMed

    Nakamura, Hiroshige

    2014-05-01

    Robot-assisted surgery using the da Vinci surgical system has attracted attention because of excellent operability without shaking by joint forceps under the clear vision of a three-dimensional high-definition camera in lung cancer surgery. Although this form of advanced medical care is not yet approved for insurance coverage, it is at the stage of clinical research and expected to be useful in hilar exposure, lymph node dissection, and suturing of the lung parenchyma or bronchus. Lung cancer surgery with the da Vinci system has the advantage of combining thoracotomy and minimally invasive surgery in video-assisted thoracic surgery. However, safety management, education, and significant cost are problems to be resolved. Several important issues such as sharing knowledge and technology of robotic surgery, education, training, development of new instruments, and acquisition of advanced medical insurance are discussed for the future development of robotic surgical systems.

  7. 16-kDa prolactin and bromocriptine in postpartum cardiomyopathy.

    PubMed

    Hilfiker-Kleiner, Denise; Struman, Ingrid; Hoch, Melanie; Podewski, Edith; Sliwa, Karen

    2012-09-01

    Peripartum cardiomyopathy (PPCM) is a potentially life-threatening heart disease emerging toward the end of pregnancy or in the first postpartal months in previously healthy women. Recent data suggest a central role of unbalanced peri-/postpartum oxidative stress that triggers the proteolytic cleavage of the nursing hormone prolactin (PRL) into a potent antiangiogenic, proapoptotic, and proinflammatory 16-kDa PRL fragment. This notion is supported by the observation that inhibition of PRL secretion by bromocriptine, a dopamine D2-receptor agonist, prevented the onset of disease in an animal model of PPCM and by first clinical experiences where bromocriptine seem to exert positive effects with respect to prevention or treatment of PPCM patients. Here, we highlight the current state of knowledge on diagnosis of PPCM, provide insights into the biology and pathophysiology of 16-kDa PRL and bromocriptine, and outline potential consequences for the clinical management and treatment options for PPCM patients.

  8. DA-9801 promotes neurite outgrowth via ERK1/2-CREB pathway in PC12 cells.

    PubMed

    Won, Jong Hoon; Ahn, Kyong Hoon; Back, Moon Jung; Ha, Hae Chan; Jang, Ji Min; Kim, Ha Hyung; Choi, Sang-Zin; Son, Miwon; Kim, Dae Kyong

    2015-01-01

    In the present study, we examined the mechanisms underlying the effect of DA-9801 on neurite outgrowth. We found that DA-9801 elicits its effects via the mitogen-activated protein kinase (MEK) extracellular signal-regulated kinase (ERK)1/2-cAMP response element-binding protein (CREB) pathway. DA-9801, an extract from a mixture of Dioscorea japonica and Dioscorea nipponica, was reported to promote neurite outgrowth in PC12 cells. The effects of DA-9801 on cell viability and expression of neuronal markers were evaluated in PC12 cells. To investigate DA-9801 action, specific inhibitors targeting the ERK signaling cascade were used. No cytotoxicity was observed in PC12 cells at DA-9801 concentrations of less than 30 µg/mL. In the presence of nerve growth factor (NGF, 2 ng/mL), DA-9801 promoted neurite outgrowth and increased the relative mRNA levels of neurofilament-L (NF-L), a marker of neuronal differentiation. The Raf-1 inhibitor GW5074 and MEK inhibitor PD98059 significantly attenuated DA-9801-induced neurite outgrowth. Additionally, the MEK1 and MEK2 inhibitor SL327 significantly attenuated the increase in the percentage of neurite-bearing PC12 cells induced by DA-9801 treatment. Conversely, the selective p38 mitogen-activated protein kinase inhibitor SB203580 did not attenuate the DA-9801 treatment-induced increase in the percentage of neurite-bearing PC12 cells. DA-9801 enhanced the phosphorylation of ERK1/2 and CREB in PC12 cells incubated with and without NGF. Pretreatment with PD98059 blocked the DA-9801-induced phosphorylation of ERK1/2 and CREB. In conclusion, DA-9801 induces neurite outgrowth by affecting the ERK1/2-CREB signaling pathway. Insights into the mechanism underlying this effect of DA-9801 may suggest novel potential strategies for the treatment of peripheral neuropathy.

  9. On the sexual intercourse drawings of Leonardo da Vinci.

    PubMed

    Morris, A G

    1986-04-12

    Leonardo da Vinci's marvellous anatomical drawings have been praised by both artists and medical historians over the centuries. Specific reference is made here to Leonardo's drawings of the act of sexual intercourse. It is shown that his illustrations are not based purely on original observation. Rather, they are an attempt to illustrate and clarify anatomy and physiology as presented in the textbooks of the time.

  10. Da Vinci's codex and the anatomy of healthcare.

    PubMed

    Stephens-Borg, Keith

    2012-08-01

    We usually display a laid-back approach to medical jargon throughout our theatre work. The word 'perioperative' is built from the Greek word 'peri' (around) and the Latin 'operari' (to work). Latin and Greek became the prefixed language of choice for Leonardo da Vinci, and his research was pivotal in determining the way in which surgical procedures are documented. Ancient manuscripts aided the unfolding of the secrets of anatomy, and Leonardo revealed that art was the key in expressive detailed explanation.

  11. Is there any mantle plume beneath Tristan da Cunha?

    NASA Astrophysics Data System (ADS)

    Schloemer, A.; Geissler, W. H.; Jegen, M. D.; Jokat, W.

    2015-12-01

    Tristan da Cunha is a volcanic island in the South Atlantic located very close to the Mid-Atlantic Ridge. Generally, it is accepted to be the location of a mantle plume, which has been active at least since the breakup of Gondwana at 130 Ma, the time when the Paraná/Etendeka flood basalts were emplaced. Furthermore, it is associated with the formation of the Walvis Ridge and the Rio Grande Rise, and therefore it's one of the key examples of a hot spot track linking a flood basalt province to an active ocean island volcano. However, global tomography models are contradicting about the origin of Tristan da Cunha: Whether it is a deep mantle plume or caused by shallow plate tectonics. To gain a better understanding, we deployed 24 broadband ocean-bottom seismometers, 26 ocean-bottom electromagnetic stations and 2 seismological land stations in January 2012 with the German research vessel Maria S. Merian. We acquired continuous seismological data for one year and recovered the instruments in January 2013.We use cross-correlated travel time residuals of teleseismic earthquakes to perform a finite-frequency tomography to resolve the P wave velocity upper mantle structure beneath the island. Here we show our preliminary results of the 3-D velocity perturbations in the upper mantle: We do not image a plume-like structure directly beneath the island. Instead we observe a low velocity region in the southwest of our array that might be related to a local mantle upwelling (mantle plume). Additionally we show the local seismicity in the Tristan da Cunha region.Chen et al. and Baba et al. will present the first results on the magnetotelluric experiment and Ryberg et al. will present the crustal structure around the Tristan da Cunha hotspot.

  12. OpenDA-NEMO framework for ocean data assimilation

    NASA Astrophysics Data System (ADS)

    van Velzen, Nils; Altaf, Muhammad Umer; Verlaan, Martin

    2016-05-01

    Data assimilation methods provide a means to handle the modeling errors and uncertainties in sophisticated ocean models. In this study, we have created an OpenDA-NEMO framework unlocking the data assimilation tools available in OpenDA for use with NEMO models. This includes data assimilation methods, automatic parallelization, and a recently implemented automatic localization algorithm that removes spurious correlations in the model based on uncertainties in the computed Kalman gain matrix. We have set up a twin experiment where we assimilate sea surface height (SSH) satellite measurements. From the experiments, we can conclude that the OpenDA-NEMO framework performs as expected and that the automatic localization significantly improves the performance of the data assimilation algorithm by successfully removing spurious correlations. Based on these results, it looks promising to extend the framework with new kinds of observations and work on improving the computational speed of the automatic localization technique such that it becomes feasible to include large number of observations.

  13. Identification of an abundant 56 kDa protein implicated in food allergy as granule-bound starch synthase

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Rice, the staple food of South and East Asian counties, is considered to be hypoallergenic. However, several clinical studies have documented rice-induced allergy in sensitive patients. Rice proteins with molecular weights of 14-16 kDa, 26 kDa, 33 kDa and 56 kDa have been identified as allergens. Re...

  14. Proving the Orbits of the Galilean Moons Through Astrophotography. (Spanish Title: Comprobando Las Órbitas de Las Lunas Galileanas a Través de la Astrofotografía.) Evidenciando as Órbitas das Luas Galileanas Atravéd da Astrofotografia

    NASA Astrophysics Data System (ADS)

    Iachel, Gustavo

    2009-12-01

    An activity related to the observation of the Galilean moons and to amateur astrophotography is presented in this work. Through the reading of excerpts of the book Sidereus Nuncius (Sidereal Messenger), by Galileo, it is possible to trace a methodology to observe the planet and its natural satellites and, with the aid of the astrophotography, to analyze the field recordings. Moreover, it is possible to compare the images obtained after plotting the relative positions of these moons to prove their orbits. This activity can be conducted by teachers, students and amateur astronomers, in order to develop their capabilities of observation of astronomical phenomena. Se presenta en este trabajo una actividad relacionada a la observación de las lunas Galileanas y a la astrofotografía amateur. A través de la lectura de tramos de la obra Sidereus Nuncius (El Mensajero Sideral) de Galileo, es posible trazar una metodología para la observación del planeta y de sus satélites naturales y, con el auxilio de la astrofotografía, analizar las anotaciones de campo. Además, es posible comparar las imágenes obtenidas con el gráfico de las posiciones relativas de estas lunas para comprobar sus órbitas. Esa actividad puede ser realizada por maestros, estudiantes y astrónomos amateurs, de forma a desarrollar sus capacidades de observación de los fenómenos astronómicos. Uma atividade relacionada à observação das luas Galileanas e à astrofotografia amadora é apresentada neste trabalho. Através da leitura de trechos da obra Sidereus Nuncius (Mensageiro Sideral), de Galileu, é possível traçar uma metodologia para a observação do planeta e de seus satélites naturais e, com o auxílio da astrofotografia, analisar as anotações de campo. Além disso, é possível comparar as imagens obtidas com o gráfico das posições relativas destas luas para evidenciar suas órbitas. Essa atividade pode ser realizada por professores, estudantes e astrônomos amadores, de forma a

  15. [The Arabic influence in the "Colóquios dos simples e drogas da India" of Garcia da Orta].

    PubMed

    Ricordel, Joëlle

    2015-09-01

    The "Colóquios dos simples e drogas he cousas medicinais de Índia" (Conversations on the simples, drugs and medicinal substances of India) (1563) of Garcia da Orta is a botanical and pharmacognosy book. The author is a Portuguese physician who studied in the Spanish universities and practiced medicine mainly in India. He studies in short chapters presented in the form of dialogues about sixty simples. Sources to which he refers are indicative of a "classical" training, but also the mark of a curious and open mind to different cultures. The Arabic sources are numerous and mainly concern the identification of substances by abundant synonyms of their names in foreign languages and different medicinal uses that may have been done by the ancient physicians. However, Da Orta is critical with respect to these sources, seeking contradictions and differences of opinion among authors. He confronts them with the oral information collected thanks to a wide network of contacts. PMID:26529894

  16. [The Arabic influence in the "Colóquios dos simples e drogas da India" of Garcia da Orta].

    PubMed

    Ricordel, Joëlle

    2015-09-01

    The "Colóquios dos simples e drogas he cousas medicinais de Índia" (Conversations on the simples, drugs and medicinal substances of India) (1563) of Garcia da Orta is a botanical and pharmacognosy book. The author is a Portuguese physician who studied in the Spanish universities and practiced medicine mainly in India. He studies in short chapters presented in the form of dialogues about sixty simples. Sources to which he refers are indicative of a "classical" training, but also the mark of a curious and open mind to different cultures. The Arabic sources are numerous and mainly concern the identification of substances by abundant synonyms of their names in foreign languages and different medicinal uses that may have been done by the ancient physicians. However, Da Orta is critical with respect to these sources, seeking contradictions and differences of opinion among authors. He confronts them with the oral information collected thanks to a wide network of contacts.

  17. Reconstitution of surfactant activity by using the 6 kDa apoprotein associated with pulmonary surfactant.

    PubMed Central

    Yu, S H; Possmayer, F

    1986-01-01

    Lipid extracts of bovine pulmonary surfactant containing the 6 kDa apoprotein, but lacking the 35 kDa apoprotein, can mimic the essential characteristics of pulmonary surfactant on a pulsating-bubble surfactometer. Reconstituted surfactant can be produced by combining silicic acid fractions containing 6 kDa apoprotein and phosphatidylglycerol with phosphatidylcholine. Treatment of the protein-containing fraction with proteolytic enzymes abolishes its efficacy. These results indicate that the presence of the 6 kDa apoprotein can account for some of the essential physical and biological characteristics of pulmonary surfactant. Immunodiffusion studies indicate that, contrary to earlier suggestions, the 6 kDa apoprotein is not structurally related to the major surfactant apoprotein that has a molecular mass of 35 kDa. Images Fig. 2. Fig. 3. Fig. 4. PMID:3098235

  18. Accumulation of 23 kDa lipocalin during brain development and injury in Hyphantria cunea.

    PubMed

    Kim, Hong Ja; Je, Hyun Jeong; Cheon, Hyang Mi; Kong, Sun Young; Han, JikHyun; Yun, Chi Young; Han, Yeon Su; Lee, In Hee; Kang, Young Jin; Seo, Sook Jae

    2005-10-01

    The cDNA corresponding to a novel lipocalin was identified from the fall webworm, Hyphantria cunea. This lipocalin cDNA encodes a 194 residue protein with a calculated molecular mass of 23 kDa. Sequence analyses revealed that the 23 kDa lipocalin cDNA is most similar to Drosophila lazarillo, human apolipoprotein D, and Bombyrin. Northern blot analyses showed that 23 kDa lipocalin transcript is expressed in the whole body only in 4- and 6-day-old pupae. By Western blot analysis it was confirmed that 23 kDa lipocalin is mainly accumulated in brain and subesophageal ganglion, though it is detected in a small amount in fat body and epidermis of Hyphantria cunea. The accumulation of 23 kDa lipocalin in brain tissue was upregulated in response to injury. The putative function of 23 kDa lipocalin in brain is discussed.

  19. An epidemic of influenza on Tristan da Cunha.

    PubMed

    Mantle, J; Tyrrell, D A

    1973-03-01

    Respiratory disease on Tristan da Cunha has been observed since the islanders returned in 1962. An epidemic of unprecedented severity occurred in the winter of 1971 and involved 273 (96%) of 284 islanders, 92 of whom had two attacks.The epidemic was apparently introduced by the Tristania.The illness of both first and second attacks ranged from mild to severe but there were some differences. There were two deaths, both in elderly persons with chronic chest disease and heart failure. Serological evidence suggests that this was due to influenza A2 of the Hong Kong serotype H(3)N(2).

  20. An epidemic of influenza on Tristan da Cunha

    PubMed Central

    Mantle, John; Tyrrell, D. A. J.

    1973-01-01

    Respiratory disease on Tristan da Cunha has been observed since the islanders returned in 1962. An epidemic of unprecedented severity occurred in the winter of 1971 and involved 273 (96%) of 284 islanders, 92 of whom had two attacks. The epidemic was apparently introduced by the Tristania. The illness of both first and second attacks ranged from mild to severe but there were some differences. There were two deaths, both in elderly persons with chronic chest disease and heart failure. Serological evidence suggests that this was due to influenza A2 of the Hong Kong serotype H3N2. PMID:4511951

  1. TA-DA: A Tool for Astrophysical Data Analysis

    NASA Astrophysics Data System (ADS)

    Da Rio, Nicola; Robberto, Massimo

    2012-10-01

    TA-DA is a pre-compiled IDL widget-based application which greatly simplifies and improves the analysis of stellar photometric data in comparison with theoretical models and allows the derivation of stellar parameters from multi-band photometry. It is flexible and can address a number of problems, from the interpolation of stellar models or sets of stellar physical parameters in general to the computation of synthetic photometry in arbitrary filters or units. It also analyzes observed color-magnitude diagrams and allows a Bayesian derivation of stellar parameters (and extinction) based on multi-band data.

  2. Leonardo da Vinci and Kethem-Kiveris vena.

    PubMed

    Dolezal, Antonín; Skorepova-Honzlova, Zita; Jelen, Karel

    2012-01-01

    In the drawing of coitus by Leonardo da Vinci are pictured the contemporary hypotheses regarding this act. The authors analyze the mamillaruteral connection depicted by the artist and grow up to believe that this is a hypothetical kiveris vena, female vein described by Anatomist Master Nicolai Physicus from the Salerno School. The Hebrew roots were found in the name. The connection is described also by Mondino in The Anathomia. The same connection can be found in the picture of the pregnant woman in Fasciculus Medicinæ by Johannes De Ketham.

  3. LEONARDO DA VINCI AND THE ORIGIN OF SEMEN.

    PubMed

    Noble, Denis; DiFrancesco, Dario; Zancani, Diego

    2014-12-20

    It is well known that Leonardo da Vinci made several drawings of the human male anatomy. The early drawings (before 1500) were incorrect in identifying the origin of semen, where he followed accepted teaching of his time. It is widely thought that he did not correct this mistake, a view that is reflected in several biographies. In fact, he made a later drawing (after 1500) in which the description of the anatomy is remarkably accurate and must have been based on careful dissection. In addition to highlighting this fact, acknowledged previously in only one other source, this article reviews the background to Leonardo's knowledge of the relevant anatomy.

  4. Visual tracking of da Vinci instruments for laparoscopic surgery

    NASA Astrophysics Data System (ADS)

    Speidel, S.; Kuhn, E.; Bodenstedt, S.; Röhl, S.; Kenngott, H.; Müller-Stich, B.; Dillmann, R.

    2014-03-01

    Intraoperative tracking of laparoscopic instruments is a prerequisite to realize further assistance functions. Since endoscopic images are always available, this sensor input can be used to localize the instruments without special devices or robot kinematics. In this paper, we present an image-based markerless 3D tracking of different da Vinci instruments in near real-time without an explicit model. The method is based on different visual cues to segment the instrument tip, calculates a tip point and uses a multiple object particle filter for tracking. The accuracy and robustness is evaluated with in vivo data.

  5. High Resolution EUV & FUV Spectroscopy of DA White Dwarfs

    NASA Astrophysics Data System (ADS)

    Barstow, M. A.; Good, S. A.; Bannister, N. P.; Burleigh, M. R.; Holberg, J. B.; Bruhweiler, F. C.; Napiwotzki, R.; Cruddace, R. G.; Kowalski, M. P.

    We report on recent results from a high-resolution spectroscopic survey of hot DA white dwarfs, based on IUE, FUSE and HST observations. For the first time, we address the measurement of element abundances in a completely objective manner with a spectroscopic model fitting technique, which allows us to consider formally the limits that can be placed on abundances in stars where no heavy elements are detected. We also include our latest analysis of the high resolution EUV spectrum of G191-B2B recorded by J-PEX.

  6. Leonardo da Vinci and the origin of semen

    PubMed Central

    Noble, Denis; DiFrancesco, Dario; Zancani, Diego

    2014-01-01

    It is well known that Leonardo da Vinci made several drawings of the human male anatomy. The early drawings (before 1500) were incorrect in identifying the origin of semen, where he followed accepted teaching of his time. It is widely thought that he did not correct this mistake, a view that is reflected in several biographies. In fact, he made a later drawing (after 1500) in which the description of the anatomy is remarkably accurate and must have been based on careful dissection. In addition to highlighting this fact, acknowledged previously in only one other source, this article reviews the background to Leonardo's knowledge of the relevant anatomy. PMID:27494016

  7. Josephson D/A converter with fundamental accuracy

    SciTech Connect

    Hamilton, C.A.; Burroughs, C.J.; Kautz, R.L.

    1994-12-31

    A binary sequence of series arrays of shunted Josephson junctions is used to make a 14-bit D/A converter. With thirteen bias lines any step number in the range -8192 to +8192 (1.2 to -1.2 V) can be selected in the time required to stabilize the bias current (a few microseconds). The circuit makes possible the digital synthesis of very-accurate ac wave-forms whose amplitude derives directly from the internationally accepted definition of the volt.

  8. Identification of sequence similarity between 60 kDa and 70 kDa molecular chaperones: evidence for a common evolutionary background?

    PubMed Central

    Flores, A I; Cuezva, J M

    1997-01-01

    Recent findings support the premise that chaperonins (60 kDa stress-proteins) and alpha-subunits of F-type ATPases (alpha-ATPase) are evolutionary related protein families. Two-dimensional gel patterns of synthesized proteins in unstressed and heat-shocked embryonic Drosophila melanogaster SL2 cells revealed that antibodies raised against the alpha-subunit of the F1-ATPase complex from rat liver recognize an inducible p71 member of the 70 kDa stress-responsive protein family. Molecular recognition of this stress-responsive 70 kDa protein by antibodies raised against the F1-ATPase alpha-subunit suggests the possibility of partial sequence similarity within these ATP-binding protein families. A multiple sequence alignment between alpha-ATPases and 60 kDa and 70 kDa molecular chaperones is presented. Statistical evaluation of sequence similarity reveals a significant degree of sequence conservation within the three protein families. The finding suggests a common evolutionary origin for the ATPases and molecular chaperone protein families of 60 kDa and 70 kDa, despite the lack of obvious structural resemblance between them. PMID:9065788

  9. Effects of DA-Phen, a dopamine-aminoacidic conjugate, on alcohol intake and forced abstinence.

    PubMed

    Sutera, Flavia Maria; De Caro, Viviana; Cannizzaro, Carla; Giannola, Libero Italo; Lavanco, Gianluca; Plescia, Fulvio

    2016-09-01

    The mesolimbic dopamine (DA) system plays a key role in drug reinforcement and is involved in the development of alcohol addiction. Manipulation of the DAergic system represents a promising strategy to control drug-seeking behavior. Previous studies on 2-amino-N-[2-(3,4-dihydroxy-phenyl)-ethyl]-3-phenyl-propionamide (DA-Phen) showed in vivo effects as a DA-ergic modulator. This study was aimed at investigate DA-Phen effects on operant behavior for alcohol seeking behavior, during reinstatement following subsequent periods of alcohol deprivation. For this purpose, male Wistar rats were tested in an operant paradigm of self-administration; behavioral reactivity and anxiety like-behavior during acute abstinence were evaluated. A characterization of DA-Phen CNS targeting by its quantification in the brain was also carried out. Our findings showed that DA-Phen administration was able to reduce relapse in alcohol drinking by 50% and reversed the alterations in behavioral reactivity and emotionality observed during acute abstinence. In conclusion, DA-Phen can reduce reinstatement of alcohol drinking in an operant-drinking paradigm following deprivation periods and reverse abstinence-induced behavioral phenotype. DA-Phen activity seems to be mediated by the modulation of the DAergic transmission. However further studies are needed to characterize DA-Phen pharmacodynamic and pharmacokinetic properties, and its potential therapeutic profile in alcohol addiction. PMID:27155501

  10. Evolution of the ATLAS PanDA workload management system for exascale computational science

    NASA Astrophysics Data System (ADS)

    Maeno, T.; De, K.; Klimentov, A.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.; Yu, D.; Atlas Collaboration

    2014-06-01

    An important foundation underlying the impressive success of data processing and analysis in the ATLAS experiment [1] at the LHC [2] is the Production and Distributed Analysis (PanDA) workload management system [3]. PanDA was designed specifically for ATLAS and proved to be highly successful in meeting all the distributed computing needs of the experiment. However, the core design of PanDA is not experiment specific. The PanDA workload management system is capable of meeting the needs of other data intensive scientific applications. Alpha-Magnetic Spectrometer [4], an astro-particle experiment on the International Space Station, and the Compact Muon Solenoid [5], an LHC experiment, have successfully evaluated PanDA and are pursuing its adoption. In this paper, a description of the new program of work to develop a generic version of PanDA will be given, as well as the progress in extending PanDA's capabilities to support supercomputers and clouds and to leverage intelligent networking. PanDA has demonstrated at a very large scale the value of automated dynamic brokering of diverse workloads across distributed computing resources. The next generation of PanDA will allow other data-intensive sciences and a wider exascale community employing a variety of computing platforms to benefit from ATLAS' experience and proven tools.

  11. Effects of DA-Phen, a dopamine-aminoacidic conjugate, on alcohol intake and forced abstinence.

    PubMed

    Sutera, Flavia Maria; De Caro, Viviana; Cannizzaro, Carla; Giannola, Libero Italo; Lavanco, Gianluca; Plescia, Fulvio

    2016-09-01

    The mesolimbic dopamine (DA) system plays a key role in drug reinforcement and is involved in the development of alcohol addiction. Manipulation of the DAergic system represents a promising strategy to control drug-seeking behavior. Previous studies on 2-amino-N-[2-(3,4-dihydroxy-phenyl)-ethyl]-3-phenyl-propionamide (DA-Phen) showed in vivo effects as a DA-ergic modulator. This study was aimed at investigate DA-Phen effects on operant behavior for alcohol seeking behavior, during reinstatement following subsequent periods of alcohol deprivation. For this purpose, male Wistar rats were tested in an operant paradigm of self-administration; behavioral reactivity and anxiety like-behavior during acute abstinence were evaluated. A characterization of DA-Phen CNS targeting by its quantification in the brain was also carried out. Our findings showed that DA-Phen administration was able to reduce relapse in alcohol drinking by 50% and reversed the alterations in behavioral reactivity and emotionality observed during acute abstinence. In conclusion, DA-Phen can reduce reinstatement of alcohol drinking in an operant-drinking paradigm following deprivation periods and reverse abstinence-induced behavioral phenotype. DA-Phen activity seems to be mediated by the modulation of the DAergic transmission. However further studies are needed to characterize DA-Phen pharmacodynamic and pharmacokinetic properties, and its potential therapeutic profile in alcohol addiction.

  12. Percepção astronómica de um grupo de alunos do ensino médio de uma escola da rede estadual de São Paulo

    NASA Astrophysics Data System (ADS)

    de Oliveria, E. F.; Voelzke, M. R.

    2009-03-01

    Sendo a Astronomia uma das cièncias mais antigas da humanidade, e considerando sua importáncia histórica e cultural, é de extrema releváncia que tópicos relacionados a ela sejam tratados nas escolas. Embora os Parámetros Curriculares Nacionais do Ensino Médio (PCN-EM) e as Orientaçiacute;es Complementares aos Parámetros (PCN+) apontem a importáncia de uma abordagem significativa de conceitos relacionados à Astronomia nas aulas de Física, muitos estudantes terminam o Ensino Médio (EM) sem compreender a razão de certos acontecimentos de origem celeste, ainda que estes façam parte de seu cotidiano e sejam alvos da curiosidade natural dos jovens. Da observação dessa curiosidade em alunos de uma escola pública paulista, na cidade de Suzano, surgiu o interesse em investigar os conhecimentos básicos em Astronomia dos alunos do Ensino Médio desta escola, constituindo-se este como principal objetivo desta pesquisa. Para tanto foi elaborado um questionário de múltipla escolha aplicado inicialmente a 34 alunos do primeiro ano e, posteriormente, a mais 310, distribuídos entre as très séries do Ensino Médio dos períodos matutino e noturno. Dessa forma, observou-se que 73,9% dos estudantes identificaram o Sol como sendo uma estrela, 67,1% mostraram compreender a sucessão entre dia e noite e 52,3% relacionaram o Big Bang à origem do Universo. Em contrapartida, apenas 34,5% relacionaram as estaçíes do ano à inclinação do eixo de rotação da Terra, 21,3% indicaram a influència gravitacional simultánea da Lua e do Sol como responsável pelo fenòmeno das marés, 24,5% indicaram corretamente quais são os objetos celestes mais próximos da Terra, 36,1% identificaram ano-luz como uma medida de distáncia e 34,2% reconheceram as estrelas cadentes como meteoros, evidenciando-se assim o pequeno discernimento dos estudantes quanto aos fenòmenos e termos astronòmicos do cotidiano. Além disso, foram comparadas as respostas de alun! os de diferentes s

  13. Chandra Confirmation of a Pulsar Wind Nebula in DA 495

    NASA Technical Reports Server (NTRS)

    Arzoumanian, Z.; Safi-Harb, S.; Landecker, T.L.; Kothes, R.; Camilo, F.

    2008-01-01

    As part of a multiwavelength study of the unusual radio supernova remnant DA 495, we present observations made with the Chandra X-ray Observatory. Imaging and spectroscopic analysis confirms the previously detected X-ray source at the heart of the annular radio nebula, establishing the radiative properties of two key emission components: a soft unresolved source with a blackbody temperature of 1 MK consistent with a neutron star, surrounded by a nontherma1 nebula 40" in diameter exhibiting a power-law spectrum with photon index Gamma = 1.63, typical of a pulsar wind nebula. Morphologically, the nebula appears to be slightly extended along a direction, in projection on the sky, previously demonstrated to be of significance in radio and ASCA observations; we argue that this represents the orientation of the pulsar spin axis. At smaller scales, a narrow X-ray feature is seen extending out 5" from the point source, but energetic arguments suggest that it is not the resolved termination shock of the pulsar wind against the ambient medium. Finally, we argue based on synchrotron lifetimes in the nebular magnetic field that DA 495 represents the first example of a pulsar wind nebula in which electromagnetic flux makes up a significant part, together with particle flux, of the neutron star's wind.

  14. DA/DSM{trademark} focuses on utility restructuring

    SciTech Connect

    Hanson, E.

    1995-03-01

    Debate about restructuring the electric industry and the proposal for retail wheeling in California dominated discussion at DA/DSM `95, the fifth annual symposium on distribution automation and demand-side management. The basic goal is to introduce competition and reduce costs by providing choices for consumers between regulated utilities and direct contracts with other providers. All customers will have some means of direct access to outside generation sources. However, for residential customers, direct access will probably come through an aggregation of interests. Focusing on the future of demand-side management, there is concern that California`s restructuring may spell doom for DSM. DSM spending has declined over the past year and a major question raised was the fate of uneconomic DSM, environmental, renewable and other social programs. There was great interest at DA/DSM `95 in sessions devoted to electric utility activity on the information superhighway, loosely defined as methods to improve communications between customers and utilities. Several projects are described.

  15. A PEG-DA microfluidic device for chemotaxis studies

    NASA Astrophysics Data System (ADS)

    Aziz Traore, Mahama; Behkam, Bahareh

    2013-08-01

    The study of cells in a well-defined and chemically programmable microenvironment is essential for a complete and fundamental understanding of the cell behaviors with respect to specific chemical compounds. Flow-free microfluidic devices that generate quasi-steady chemical gradients (spatially varying but temporally constant) have been demonstrated as effective chemotaxis assay platforms due to dissociating the effect of chemical cues from mechanical shear forces caused by fluid flow. In this work, we demonstrate the fabrication and characterization of a flow-free microfluidic platform made of polyethylene glycol diacrylate (PEG-DA) hydrogel. We have demonstrated that the mass transport properties of these devices can be customized by fabricating them from PEG-DA gels of four distinct molecular weights. In contrast to microfluidic devices developed using soft lithography; this class of devices can be realized using a more cost-effective approach of direct photopolymerization with fewer microfabrication steps. This microfluidic platform was tested by conducting a quantitative study of the chemotactic behavior of Escherichia coli (E. coli) RP437, a model microorganism, in presence of the chemo-effector, casamino-acids. Using the microfabrication and characterization methodology presented in this work, microfluidic platforms with well-defined and customizable diffusive properties can be developed to accommodate the study of a wide range of cell types.

  16. Condições físicas do disco de acréscimo da nova-anã V4140 SGR

    NASA Astrophysics Data System (ADS)

    Borges, B.; Baptista, R.

    2003-08-01

    Discos de acréscimo são aparatos cósmicos que permitem que matéria seja eficientemente acrescida sobre uma fonte compacta pela remoção de momento angular via tensões viscosas enquanto transforma a energia potencial gravitacional em calor e, posteriormente, em radiação. Sistemas binários semi-ligados, como Variáveis Cataclísmicas (VCs) não-magnéticas, são talvez os melhores ambientes encontrados para o estudo da física desses discos de acréscimo. O desenvolvimento de técnicas de imageamento indireto, como o mapeamento por eclipses (MME) e a tomografia Doppler, permitiu avanços importantes na compreensão da física dos processos de acréscimo desses sistemas. V4140 Sagitarii é uma VC eclipsante de curto período orbital (~ 90 min) que não havia sido classificada como nova-anã ou polar. Neste trabalho apresentamos a análise do mapeamento por eclipse feito com dados de fotometria CCD nas bandas B, V e R de V4140 Sgr realizada no LNA. O objeto foi observado no declínio de erupção em julho de 1992 e em erupção em julho de 2001, isso indicou a classificação do sistema como uma nova-anã. A análise, feita sobre os mapas já apresentados em trabalho anterior, apresenta (i) diagrama cor-cor, que indica uma emissão opticamente espessa nas partes internas do disco em quiescência (R < RL1); (ii) diagrama cor-magnitude, usado para inferir uma distância de 600 +/- 10 pc ao sistema; (iii) a distribuição radial de temperatura observada que concorda com a lei de disco espesso em estado estacionário TµR-3/4, com temperaturas em torno de 10000 K e 3000 K ns partes internas e externas do disco em quiescência, respectivamente; (iv) a evolução da distribuição de temperatura no declínio de erupção; (v) estimativa da transferência de massa de 109.9+/-0.1 M¤/ano em quiescência, similar àquelas observadas em novas-like. O sistema é comparado à outras novas-anãs (e.g. OY Car e Z Cha) em quiescência e erupção, mostrando-se tratar um

  17. D-A and D-2 dopamine receptor function in the rabbit retina: a model for the central nervous system

    SciTech Connect

    Hensler, J.G.

    1987-01-01

    Studies were done investigating the effect of the synaptic concentration of the transmitter DA, modified by changes in the frequency of electrical field stimulation and by the DA uptake inhibitor nomifensine, on the modulation of /sup 3/H-DA release by D-2 DA autoreceptors and by melatonin receptor sites. At lower synaptic concentrations of the transmitter dopamine, D-2 DA receptor agonists were more potent, while antagonists were more potent when the synaptic concentration of transmitter was higher. The potency of melatonin to inhibit DA release was not altered by the frequency of field stimulation of by frequency-dependent changes in the synaptic concentration of the transmitter.

  18. 28 kDa adenosine-binding proteins of brain and other tissues.

    PubMed Central

    Ravid, K; Rosenthal, R A; Doctrow, S R; Lowenstein, J M

    1989-01-01

    Membranes prepared from calf brain were solubilized and chromatographed on a column containing 5'-amino-5'-deoxyadenosine covalently linked to agarose through the 5'-amino group. When the column was eluted with adenosine, a pure protein emerged with subunit molecular mass of 28 kDa. The protein was extracted from the membranes with sodium cholate, but not with 100 microM-adenosine or 0.5 M-NaCl. A similar 28 kDa protein was isolated from the soluble fraction of calf brain. The yield of membrane-bound and soluble 28 kDa protein per gram of tissue was about the same. The 28 kDa protein was also found in membrane and soluble fractions of rabbit heart, rat liver and vascular smooth muscle from calf aorta. The yield per gram of tissue fell into the order brain greater than heart approximately vascular smooth muscle greater than liver for the 28 kDa protein from the membrane fraction, and brain approximately heart greater than vascular smooth muscle greater than liver for the 28 kDa protein from the soluble fraction. Polyclonal antibodies to pure 28 kDa protein from calf brain membranes cross-reacted with the 28 kDa protein from calf brain soluble fraction and with 28 kDa proteins isolated from other tissues. The 28 kDa protein from calf brain membranes was also eluted from the affinity column by AMP and 2',5'-dideoxyadenosine, but at a concentration higher than that at which adenosine eluted the protein, but N6-(R-phenylisopropyl)adenosine, 5'-N-ethylcarboxamidoadenosine, ADP, ATP, GTP, NAD+, cyclic AMP and inosine failed to elute the protein at concentrations up to 1 mM. The 28 kDa protein from the soluble fraction was not eluted by 3 mM-AMP or 1 mM-N6-(R-phenylisopropyl)adenosine,-5'-N-ethylcarboxamidoadenosine or -cyclic AMP. Unexpectedly, the soluble 28 kDa protein was eluted by AMP in the presence of sodium cholate. Soluble 28 kDa protein from calf brain had a KD for adenosine of 12 microM. Membrane 28 kDa protein from calf brain had a KD of 14 microM in the

  19. Axonal patterns and targets of dA1 interneurons in the chick hindbrain.

    PubMed

    Kohl, Ayelet; Hadas, Yoav; Klar, Avihu; Sela-Donenfeld, Dalit

    2012-04-25

    Hindbrain dorsal interneurons that comprise the rhombic lip relay sensory information and coordinate motor outputs. The progenitor dA1 subgroup of interneurons, which is formed along the dorsal-most region of the caudal rhombic lip, gives rise to the cochlear and precerebellar nuclei. These centers project sensory inputs toward upper-brain regions. The fundamental role of dA1 interneurons in the assembly and function of these brainstem nuclei is well characterized. However, the precise en route axonal patterns and synaptic targets of dA1 interneurons are not clear as of yet. Novel genetic tools were used to label dA1 neurons and trace their axonal trajectories and synaptic connections at various stages of chick embryos. Using dA1-specific enhancers, two contralateral ascending axonal projection patterns were identified; one derived from rhombomeres 6-7 that elongated in the dorsal funiculus, while the other originated from rhombomeres 2-5 and extended in the lateral funiculus. Targets of dA1 axons were followed at later stages using PiggyBac-mediated DNA transposition. dA1 axons were found to project and form synapses in the auditory nuclei and cerebellum. Investigation of mechanisms that regulate the patterns of dA1 axons revealed a fundamental role of Lim-homeodomain (HD) proteins. Switch in the expression of the specific dA1 Lim-HD proteins Lhx2/9 into Lhx1, which is typically expressed in dB1 interneurons, modified dA1 axonal patterns to project along the routes of dB1 subgroup. Together, the results of this research provided new tools and knowledge to the assembly of trajectories and connectivity of hindbrain dA1 interneurons and of molecular mechanisms that control these patterns.

  20. 32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 3 2014-07-01 2014-07-01 false Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form...

  1. 32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 3 2013-07-01 2013-07-01 false Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form...

  2. 32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 3 2012-07-01 2009-07-01 true Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form...

  3. 40 CFR 60.44Da - Standard for nitrogen oxides (NOX).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Standard for nitrogen oxides (NOX). 60.44Da Section 60.44Da Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS... for nitrogen oxides (NOX). (a) On and after the date on which the initial performance test...

  4. A Proposal to Build Evaluation Capacity at the Bunche-Da Vinci Learning Partnership Academy

    ERIC Educational Resources Information Center

    King, Jean A.

    2005-01-01

    The author describes potential evaluation capacity-building activities in contrast to the specifics of an evaluation design. Her response to the case of the Bunche-Da Vinci Learning Partnership Academy is developed in three parts: (1) an initial framing of the Bunche-Da Vinci situation; (2) what should be done before signing a contract; and (3)…

  5. Expression and purification of 15 kDa granulysin utilizing an insect cell secretion system.

    PubMed

    Finn, Michael W; Clayberger, Carol; Krensky, Alan M

    2011-01-01

    Granulysin is an antimicrobial and proinflammatory protein expressed in activated human T cells and natural killer cells. A single mRNA produces the 15 kDa isoform which is then cleaved at the amino and carboxy termini to produce the 9 kDa isoform. Recombinant 9 kDa granulysin has been studied in detail but little is known about the function of the 15 kDa isoform, and no protocol has been published describing expression and purification of this form. Two commercially available preparations of the recombinant 15 kDa granulysin contain tags that may affect function. Here we describe for the first time a method to produce 15 kDa granulysin as a secreted protein from insect cells. The 15 kDa granulysin is purified using a HiTrap Heparin column and a Resource S column. A typical a yield of purified 15 kDa granulysin is 0.6 mg/L of insect cell supernatant.

  6. 32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form...

  7. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form...

  8. A Creative Approach to the Common Core Standards: The Da Vinci Curriculum

    ERIC Educational Resources Information Center

    Chaucer, Harry

    2012-01-01

    "A Creative Approach to the Common Core Standards: The Da Vinci Curriculum" challenges educators to design programs that boldly embrace the Common Core State Standards by imaginatively drawing from the genius of great men and women such as Leonardo da Vinci. A central figure in the High Renaissance, Leonardo made extraordinary contributions as a…

  9. 32 CFR Appendix E to Part 623 - Surety Bond (DA Form 4881-3-R)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Surety Bond (DA Form 4881-3-R) E Appendix E to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. E Appendix E to Part 623—Surety Bond (DA Form...

  10. Paśu Ayurvĕda (veterinary medicine) in Garudapurăņa.

    PubMed

    Varanasi, Subhose; Narayana, A

    2007-01-01

    The history of veterinary medicine is closely tied to the development of human medicine. Evidence of animal medicine has been found in ancient civilizations, such as those of the Hindu, Babylonians, Hebrews, Arabs, Greeks, and Romans. Ancient Indian literature in the form of the holy Vĕda, Purăna, Brăhmaņa, epics, etc. is flooded with information on animal care. The Purăņa are ancient scriptures discuss varied topics like devotion to God and his various aspects, traditional sciences like Ayurvĕda, Jyŏtişa (Astrology), cosmology, concepts like dharma, karma, reincarnation and many others. The treatment of animal diseases using Ayurvedic medicine has been mentioned in Garudapurăna, Agnipurăņa, Atri-samhită, Matsyapurăņa and many other texts. The Garudapurăņa is one of the important Săttvika purăna, the subject matter is divided into two parts, viz. Pŭrvakhaņda (first part) and an Uttarakhaņda (subsequent part). Gavăyurvĕda, Gajăyurvĕda narrated briefly and Aśvăyurvĕda described detailly in Pŭrvakhaņda. PMID:19580108

  11. The Indicative and Subjunctive "da"-complements in Serbian A Syntactic-Semantic Approach

    ERIC Educational Resources Information Center

    Todorovic, Natasa

    2012-01-01

    A syntactic-semantic investigation of subjunctive and indicative "da"-complements in Serbian is conducted in this project. After a careful comparison of Serbian sentence constructions with "da"-complements to the equivalent sentence structures in languages of the Balkans as well as other Slavic languages, it is clearly…

  12. Binding and endocytosis of 39 kDa protein by MDBK cells.

    PubMed

    Vettenranta, K; Bu, G; Schwartz, A L

    1995-08-01

    A 39 kDa protein copurifies with the low density lipoprotein receptor-related protein (LRP) and regulates ligand interactions with LRP. In our recent studies on the clearance of the 39 kDa protein in vivo, we demonstrated that once the liver LRP receptors were saturated, the kidney became the major organ responsible for the 39 kDa protein clearance (Warshawsky et al., 1993, J. Clin. Invest., 92:937-944). The current study was undertaken in order to investigate the potential binding and cellular processing of the 39 kDa protein by kidney-derived MDBK cells. Herein we demonstrate specific, high-affinity, saturable, and Ca(2+)-dependent binding of the 125I-39 kDa protein to MDBK cells (Kd approximately 10-15 nM, 50-70,000 binding sites per cell). Cellular uptake and degradation of the 125I-39 kDa protein by MDBK cells was also demonstrated with kinetics typical of receptor-mediated endocytosis. Using chemical crosslinking we show that LRP in part mediates the binding of 125I-39 kDa protein to the MDBK cell surface. In addition, the presence of functional LRP on the MDBK cell surface was confirmed by the specific binding of activated alpha 2-macroglobulin, another ligand of LRP. Our data thus demonstrate the ability of kidney-derived MDBK cells to specifically bind, endocytose, and degrade the 39 kDa protein.

  13. Growth-Promoting Hormone DA-6 Assists Phytoextraction and Detoxification of Cd by Ryegrass.

    PubMed

    He, Shanying; Wu, Qiuling; He, Zhenli

    2015-01-01

    A pot experiment was carried out to study the effect of growth-promoting hormone diethyl aminoethyl hexanoate (DA-6) on Cd phytoextraction and detoxification in ryegrass. Foliar spray of DA-6 significantly enhanced Cd extraction efficiency (P<0.05), with 1 μM DA-6 the most effective. At the subcellular level, 43-53% of Cd was soluble fraction and 23-46% in cell wall, and 9-25% in organelles. Chemical speciation analysis showed that 52.7-58.5% of Cd was NaCl extractable, 12.1-22.7% ethanol extractable, followed by other fractions. DA-6 alleviated metal toxicity by fixing more Cd in cell wall and decreasing Cd migration in plant. In conclusion, ryegrass tolerates Cd by cell wall compartmentalization along with protein and organic acids combination, and the treatment of 1 μM DA-6 appears to be optimal for enhancing the remediation efficiency of ryegrass for Cd contaminated soil.

  14. Kinship structure and heterozygosity on Tristan da Cunha.

    PubMed Central

    Thompson, E A; Roberts, D F

    1980-01-01

    Although there are many factors influencing the genotype proportions in a small population [1], a heterozygote excess, caused by avoidance of incestuous matings, will often be the expected observation. In the population of the small South-Atlantic islands of Tristan da Cunha, such an excess if observed [2], and we have investigated its origin, partitioning the population by sex and by generation. Although for such a small population the test for random pairing of genes [3, 4] is not sufficiently powerful to produce significant results, the pattern of homozygote deficiency is suggestive of avoidance of close matings. We have also investigated the effect of current nuclear family structure and family-size distribution. PMID:7386464

  15. Sine ars scientia nihil est: Leonardo da Vinci and beyond.

    PubMed

    Kickhöfel, Eduardo H P

    2009-01-01

    The aim of this article is to reflect on the relationship between art and science so far as it concerns a symposium on neurosciences. We undertake a historical overview of that relationship, paying particular attention to the sui generis case of Leonardo da Vinci, who very often is regarded as the man who worked on art and science with equal ease. We then explain why his idea of merging these two forms of knowledge failed, considering the clear-cut distinction between art and science in his time. With this clarification, we explore the matter today. We look at Raphael's The Transfiguration, in which the representation of the possessed boy is seen by neuroscientists as indicative of an epileptic seizure. We also look at the ideas of neuroscientists Semir Zeki and Vilayanur Ramachandran, who study particular aspects of brain function and suggest a new merging of art and science.

  16. Leonardo da Vinci: the search for the soul.

    PubMed

    Del Maestro, R F

    1998-11-01

    The human race has always contemplated the question of the anatomical location of the soul. During the Renaissance the controversy crystallized into those individuals who supported the heart ("cardiocentric soul") and others who supported the brain ("cephalocentric soul") as the abode for this elusive entity. Leonardo da Vinci (1452-1519) joined a long list of other explorers in the "search for the soul." The method he used to resolve this anatomical problem involved the accumulation of information from ancient and contemporary sources, careful notetaking, discussions with acknowledged experts, and his own personal search for the truth. Leonardo used a myriad of innovative methods acquired from his knowledge of painting, sculpture, and architecture to define more clearly the site of the "senso comune"--the soul. In this review the author examines the sources of this ancient question, the knowledge base tapped by Leonardo for his personal search for the soul, and the views of key individuals who followed him.

  17. [Regarding the Manuscript D " Dell' occhio " of Leonardo da Vinci].

    PubMed

    Heitz, Robert F

    2009-01-01

    Leonardo da Vinci's Manuscript D consists of five double pages sheets, which, folded in two, comprise ten folios. This document, in the old Tuscan dialect and mirror writing, reveals the ideas of Leonardo on the anatomy of the eye in relation to the formation of images and visual perception. Leonardo explains in particular the behavior of the rays in the eye in terms of refraction and reflection, and is very mechanistic in his conception of the eye and of the visual process. The most significant innovations found in these folios are the concept of the eye as a camera obscura and the intersection of light rays in the interior of the eye. His texts nevertheless show hesitation, doubts and a troubled confusion, reflecting the ideas and uncertainties of his era. He did not share his results in his lifetime, despite both printing and etching being readily available to him.

  18. Evolving understanding of translocator protein 18 kDa (TSPO).

    PubMed

    Li, Fei; Liu, Jian; Garavito, R Michael; Ferguson-Miller, Shelagh

    2015-09-01

    The translocator protein 18 kDa (TSPO) has been the focus of intense research by the biomedical community and the pharmaceutical industry because of its apparent involvement in many disease-related processes. These include steroidogenesis, apoptosis, inflammation, neurological disease and cancer, resulting in the use of TSPO as a biomarker and its potential as a drug target. Despite more than 30 years of study, the precise function of TSPO remains elusive. A recent breakthrough in determining the high-resolution crystal structures of bacterial homologs of mitochondrial TSPO provides new insight into the structural and functional properties at a molecular level and new opportunities for investigating the significance of this ancient and highly conserved protein family. The availability of atomic level structural information from different species also provides a platform for structure-based drug development. Here we briefly review current knowledge regarding TSPO and the implications of the new structures with respect to hypotheses and controversies in the field.

  19. DBS -- An rlogin multiplexer and output logger for DA systems

    SciTech Connect

    Oleynik, G.; Appleton, L.; Udumula, L.; Votava, M.

    1994-04-01

    DART Bootstrap Services (dbs) is the first component of run-control for the DART Data Acquisition system -- the DA for the 96` round of experiments at Fermilab -- though it has potential usefulness as a powerful tool in other distributed applications. dbs is an rlogin session multiplexer. It allows a user, running a single program, to start up any number of remote login sessions, feed shell commands to them, and collect their output into a single (or multiple) log files (a server keeps the sessions open and collects their output). From this program, any session can be attached to interactively so it appears just like an rlogin session -- dbs becomes transparent. When finished with this interactive mode, the user can escape back to dbs and attach to a different session if so desired. Among many other useful features, dbs supplies a mechanism for cleanup (deletion) of all processes created under a session, allowing a fresh start.

  20. A 92-kDa human immunostimulatory protein.

    PubMed Central

    Fontan, E; Briend, E; Saklani-Jusforgues, H; d'Alayer, J; Vandekerckhove, J; Fauve, R M

    1994-01-01

    We purified to apparent homogeneity a human urinary glycoprotein of 92 kDa (HGP.92) that, administered intravenously at 250 micrograms/kg, fully protected mice against a lethal inoculum of Listeria monocytogenes. Since HGP.92 protected scid mice, which lack B and T lymphocytes, this increased resistance to Listeria did not appear to be lymphocyte mediated. Furthermore, inflammatory macrophages incubated with 6 nM HGP.92 inhibited the growth of Lewis carcinoma cells in vitro. These two activities appeared to depend on an oligosaccharide moiety, as they were lost after N-Glycanase treatment of HGP.92. Thus, the biological activity of HGP.92 was in some way related to a glycan moiety. Images PMID:8078887

  1. VLBI Radar of the 2012 DA14 Asteroid

    NASA Astrophysics Data System (ADS)

    Nechaeva, M. B.; Dugin, N. A.; Antipenko, A. A.; Bezrukov, D. A.; Bezrukov, V. V.; Voytyuk, V. V.; Dement'ev, A. F.; Jekabsons, N.; Klapers, M.; Konovalenko, A. A.; Kulishenko, V. F.; Nabatov, A. S.; Nesteruk, V. N.; Putillo, D.; Reznichenko, A. M.; Salerno, E.; Snegirev, S. D.; Tikhomirov, Yu. V.; Khutornoy, R. V.; Skirmante, K.; Shmeld, I.; Chagunin, A. K.

    2015-03-01

    An experiment on VLBI radar of the 2012 DA14 asteroid was carried out on February 15-16, 2011 at the time of its closest approach to the Earth. The research teams of Kharkov (Institute of Radio Astronomy of the National Academy of Sciences of Ukraine), Evpatoria (National Space Facilities Control and Test Center), Nizhny Novgorod (Radiophysical Research Institute), Bologna (Istituto di Radioastronomia (INAF)), and Ventspils (Ventspils International Radioastronomy Center) took part in the experiment. The asteroid was irradiated by the RT-70 planetary radar (Evpatoria) at a frequency of 5 GHz. The reflected signal was received using two 32-m radio telescopes in Medicina (Italy) and Irbene (Latvia) in radiointerferometric mode. The Doppler frequency shifts in bi-static radar mode and interference frequency in VLBI mode were measured. Accuracy of the VLBI radar method for determining the radial and angular velocities of the asteroid were estimated.

  2. Pharmacological action of DA-9701 on the motility of feline stomach circular smooth muscle.

    PubMed

    Nguyen, Thanh Thao; Song, Hyun Ju; Ko, Sung Kwon; Sohn, Uy Dong

    2015-03-01

    DA-9701, a new prokinetic agent for the treatment of functional dyspepsia, is formulated with Pharbitis semen and Corydalis tuber. This study wasconducted to determine the pharmacological action of DA-9701 and to identify the receptors involved in DA-9701 -induced contractile responsesin the feline gastric corporal, fundic and antral circular smooth muscle. Concentration-response curve to DA-9701 was established. The tissue trips were exposed to methylsergide, ketanserin, ondansetron, GR 113808, atropine and dopamine before administration of DA-9701. The contractile force was determined before and after administration of drugs by a polygraph.DA-9701 enhanced the spontaneous contractile amplitude of antrum, corpus and fundus. However, it did not change the spontaneous contractile frequency of antrum and corpus, but concentration-dependently reduced that of fundus. In the fundus, DA-9701 -induced tonic contractions were inhibited by dopamine, methylsergide, ketanserine, ondansetron or GR 113808 respectively, but not by atropine, indicating that the contractile responses are mediated by multiple receptors: 5-HT2, 5-HT3, 5-HT4, and dopamine receptors. In the corpus, DA-9701-induced contractions were blocked by atropine, dopamine or GR 113808, but not by methysergide, ketanserin or ondansetron, indicating that they are involved in receptors on both, smooth muscles and neurons: 5-HT4 and dopamine receptors. However, contractile responses to DA-9701 are mainly mediated by dopamine receptors in the antrum. These results suggest that DA-9701 has important roles in gastric accommodation by enhancing tonic activity of fundus, and in gastric emptying and gastrointestinal transit by phasic contractions of corpus and antrum mediated by multiple receptors. PMID:25980179

  3. Human MHC class I antigens are associated with a 90-kDa cell surface protein.

    PubMed

    Ferm, M T; Grönberg, A

    1991-08-01

    Human MHC class I proteins are expressed on almost all nucleated cells as a heavy chain (about 45 kDa) non-covalently associated with beta 2-microglobulin (12 kDa). In this report we show that MHC class I (MHC-I) proteins can also be associated with a 90-kDa protein in the cell membrane. Surface-radiolabelled cells were treated with dithiobis succinimidyl propionate (DSP) in order to preserve multimer protein complexes during cell lysis. The lysates were immunoprecipitated and analysed by SDS-PAGE and autoradiography. Immunoprecipitation of human MHC-I proteins co-precipitated another protein of about 90 kDa in molecular weight-p90. p90 was coprecipitated from all the MHC-I expressing cells tested: U937, Raji, Molt-4 and IFN-gamma treated K562, but not from untreated, MHC-I negative K562. A 90-kDa protein was also co-precipitated with MHC-I from fresh peripheral blood mononuclear cells (PBMC). Furthermore, p90 was coprecipitated by different MoAbs to the MHC-I heavy chain or beta 2-microglobulin, but not by control antibodies. Two additional co-precipitating proteins at 34 kDa and 28 kDa were seen in MHC-I precipitates from Raji cells. Our results suggest that MHC-I proteins and the 90-kDa protein are associated in the cell membrane, probably by a close but weak, non-covalent interaction. Two additional cell surface proteins at 34 kDa and 28 kDa seem to be MHC-I associated on Raji Burkitt's lymphoma cells.

  4. Reduced striatal dopamine DA D2 receptor function in dominant-negative GSK-3 transgenic mice.

    PubMed

    Gomez-Sintes, Raquel; Bortolozzi, Analia; Artigas, Francesc; Lucas, José J

    2014-09-01

    Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase with constitutive activity involved in cellular architecture, gene expression, cell proliferation, fate decision and apoptosis, among others. GSK-3 expression is particularly high in brain where it may be involved in neurological and psychiatric disorders such as Alzheimer׳s disease, bipolar disorder and major depression. A link with schizophrenia is suggested by the antipsychotic drug-induced GSK-3 regulation and by the involvement of the Akt/GSK-3 pathway in dopaminergic neurotransmission. Taking advantage of the previous development of dominant negative GSK-3 transgenic mice (Tg) showing a selective reduction of GSK-3 activity in forebrain neurons but not in dopaminergic neurons, we explored the relationship between GSK-3 and dopaminergic neurotransmission in vivo. In microdialysis experiments, local quinpirole (DA D2-R agonist) in dorsal striatum reduced dopamine (DA) release significantly less in Tg mice than in wild-type (WT) mice. However, local SKF-81297 (selective DA D1-R agonist) in dorsal striatum reduced DA release equally in both control and Tg mice indicating a comparable function of DA D1-R in the direct striato-nigral pathway. Likewise, systemic quinpirole administration - acting preferentially on presynaptic DA D2- autoreceptors to modulate DA release-reduced striatal DA release similarly in both control and Tg mice. Quinpirole reduced locomotor activity and induced c-fos expression in globus pallidus (both striatal DA D2-R-mediated effects) significantly more in WT than in Tg mice. Taking together, the present results show that dominant negative GSK-3 transgenic mice show reduced DA D2-R-mediated function in striatum and further support a link between dopaminergic neurotransmission and GSK-3 activity.

  5. Pharmacological action of DA-9701 on the motility of feline stomach circular smooth muscle.

    PubMed

    Nguyen, Thanh Thao; Song, Hyun Ju; Ko, Sung Kwon; Sohn, Uy Dong

    2015-03-01

    DA-9701, a new prokinetic agent for the treatment of functional dyspepsia, is formulated with Pharbitis semen and Corydalis tuber. This study wasconducted to determine the pharmacological action of DA-9701 and to identify the receptors involved in DA-9701 -induced contractile responsesin the feline gastric corporal, fundic and antral circular smooth muscle. Concentration-response curve to DA-9701 was established. The tissue trips were exposed to methylsergide, ketanserin, ondansetron, GR 113808, atropine and dopamine before administration of DA-9701. The contractile force was determined before and after administration of drugs by a polygraph.DA-9701 enhanced the spontaneous contractile amplitude of antrum, corpus and fundus. However, it did not change the spontaneous contractile frequency of antrum and corpus, but concentration-dependently reduced that of fundus. In the fundus, DA-9701 -induced tonic contractions were inhibited by dopamine, methylsergide, ketanserine, ondansetron or GR 113808 respectively, but not by atropine, indicating that the contractile responses are mediated by multiple receptors: 5-HT2, 5-HT3, 5-HT4, and dopamine receptors. In the corpus, DA-9701-induced contractions were blocked by atropine, dopamine or GR 113808, but not by methysergide, ketanserin or ondansetron, indicating that they are involved in receptors on both, smooth muscles and neurons: 5-HT4 and dopamine receptors. However, contractile responses to DA-9701 are mainly mediated by dopamine receptors in the antrum. These results suggest that DA-9701 has important roles in gastric accommodation by enhancing tonic activity of fundus, and in gastric emptying and gastrointestinal transit by phasic contractions of corpus and antrum mediated by multiple receptors.

  6. Work-family conflict and time use: psychometric assessment of an instrument in ELSA-Brazil.

    PubMed

    Pinto, Karina Araujo; Menezes, Greice Maria de Souza; Griep, Rosane Härter; Lima, Keury Thaisana Rodrigues Dos Santos; Almeida, Maria da Conceição; Aquino, Estela M L

    2016-07-01

    In this study, we evaluated the psychometric properties of the items to measure the work-family conflict and the time use for personal care and leisure, included in the baseline questionnaire of the Longitudinal Study of Adult Health (ELSA-Brazil). We evaluated temporal stability (7-14 days) using kappa statistic and the validity of the construct by the correlation of Kendall's tau with other variables. Test-retest stability was discreet to moderate and the correlations were compatible with the underlying theory. Future studies in the context of ELSA-Brazil and in other populations will complement the assessment of its relevance. RESUMO Neste estudo, avaliamos as propriedades psicométricas dos itens para mensurar o conflito trabalho-família e o uso do tempo para cuidado pessoal e lazer, incluídos no questionário da linha de base do Estudo Longitudinal de Saúde do Adulto (ELSA-Brasil). Foram avaliadas a estabilidade temporal (7-14 dias) utilizando estatística kappa e a validade do construto pela correlação tau de Kendall com outras variáveis. A estabilidade teste-reteste foi discreta a moderada e as correlações, compatíveis com a teoria subjacente. Estudos futuros no contexto do ELSA-Brasil e em outras populações complementarão a avaliação da sua pertinência.

  7. Work-family conflict and time use: psychometric assessment of an instrument in ELSA-Brazil.

    PubMed

    Pinto, Karina Araujo; Menezes, Greice Maria de Souza; Griep, Rosane Härter; Lima, Keury Thaisana Rodrigues Dos Santos; Almeida, Maria da Conceição; Aquino, Estela M L

    2016-07-01

    In this study, we evaluated the psychometric properties of the items to measure the work-family conflict and the time use for personal care and leisure, included in the baseline questionnaire of the Longitudinal Study of Adult Health (ELSA-Brazil). We evaluated temporal stability (7-14 days) using kappa statistic and the validity of the construct by the correlation of Kendall's tau with other variables. Test-retest stability was discreet to moderate and the correlations were compatible with the underlying theory. Future studies in the context of ELSA-Brazil and in other populations will complement the assessment of its relevance. RESUMO Neste estudo, avaliamos as propriedades psicométricas dos itens para mensurar o conflito trabalho-família e o uso do tempo para cuidado pessoal e lazer, incluídos no questionário da linha de base do Estudo Longitudinal de Saúde do Adulto (ELSA-Brasil). Foram avaliadas a estabilidade temporal (7-14 dias) utilizando estatística kappa e a validade do construto pela correlação tau de Kendall com outras variáveis. A estabilidade teste-reteste foi discreta a moderada e as correlações, compatíveis com a teoria subjacente. Estudos futuros no contexto do ELSA-Brasil e em outras populações complementarão a avaliação da sua pertinência. PMID:27384968

  8. Endogenous dopamine (DA) modulates (3H)spiperone binding in vivo in rat brain

    SciTech Connect

    Bischoff, S.; Krauss, J.; Grunenwald, C.; Gunst, F.; Heinrich, M.; Schaub, M.; Stoecklin, K.V.; Vassout, A.; Waldmeier, P.; Maitre, L. )

    1991-01-01

    (3H)spiperone (SPI) binding in vivo, biochemical parameters and behavior were measured after modulating DA levels by various drug treatments. DA releasers and uptake inhibitors increased SPI binding in rat striatum. In other brain areas, the effects were variable, but only the pituitary remained unaffected. Surprisingly, nomifensine decreased SPI binding in frontal cortex. The effects of these drugs were monitored by measuring DA, serotonin (5-HT) and their metabolites in the same rats. The increased SPI binding in striatum was parallel to the locomotor stimulation with the following rank order: amfonelic acid greater than nomifensine greater than D-amphetamine greater than or equal to methylphenidate greater than amineptine greater than bupropion. Decreasing DA levels with reserpine or alpha-methyl-para-tyrosine reduced SPI binding by 45% in striatum only when both drugs were combined. In contrast, reserpine enhanced SPI binding in pituitary. Thus, the amount of releasable DA seems to modulate SPI binding characteristics. It is suggested that in vivo, DA receptors are submitted to dynamic regulation in response to changes in intrasynaptic concentrations of DA.

  9. Essential Roles of Da Transactivation Domains in Neurogenesis and in E(spl)-Mediated Repression

    PubMed Central

    Zarifi, Ioanna; Kiparaki, Marianthi; Koumbanakis, Konstantinos A.; Giagtzoglou, Nikolaos; Zacharioudaki, Evanthia; Alexiadis, Anastasios; Livadaras, Ioannis

    2012-01-01

    E proteins are a special class of basic helix-loop-helix (bHLH) proteins that heterodimerize with many bHLH activators to regulate developmental decisions, such as myogenesis and neurogenesis. Daughterless (Da) is the sole E protein in Drosophila and is ubiquitously expressed. We have characterized two transcription activation domains (TADs) in Da, called activation domain 1 (AD1) and loop-helix (LH), and have evaluated their roles in promoting peripheral neurogenesis. In this context, Da heterodimerizes with proneural proteins, such as Scute (Sc), which is dynamically expressed and also contributes a TAD. We found that either one of the Da TADs in the Da/Sc complex is sufficient to promote neurogenesis, whereas the Sc TAD is incapable of doing so. Besides its transcriptional activation role, the Da AD1 domain serves as an interaction platform for E(spl) proteins, bHLH-Orange family repressors which antagonize Da/Sc function. We show that the E(spl) Orange domain is needed for this interaction and strongly contributes to the antiproneural activity of E(spl) proteins. We present a mechanistic model on the interplay of these bHLH factors in the context of neural fate assignment. PMID:22949507

  10. Vectra DA for the objective measurement of disease activity in patients with rheumatoid arthritis.

    PubMed

    Segurado, O G; Sasso, E H

    2014-01-01

    Quantitative and regular assessment of disease activity in rheumatoid arthritis (RA) is required to achieve treatment targets such as remission and to optimize clinical outcomes. To assess inflammation accurately, predict joint damage and monitor treatment response, a measure of disease activity in RA should reflect the pathological processes resulting in irreversible joint damage and functional disability. The Vectra DA blood test is an objective measure of disease activity for patients with RA. Vectra DA provides an accurate, reproducible score on a scale of 1 to 100 based on the concentrations of 12 biomarkers that reflect the pathophysiologic diversity of RA. The analytical validity, clinical validity, and clinical utility of Vectra DA have been evaluated for patients with RA in registries and prospective and retrospective clinical studies. As a biomarker-based instrument for assessing disease activity in RA, the Vectra DA test can help monitor therapeutic response to methotrexate and biologic agents and assess clinically challenging situations, such as when clinical measures are confounded by non-inflammatory pain from fibromyalgia. Vectra DA scores correlate with imaging of joint inflammation and are predictive for radiographic progression, with high Vectra DA scores being associated with more frequent and severe progression and low scores being predictive for non-progression. In summary, the Vectra DA score is an objective measure of RA disease activity that quantifies inflammatory status. By predicting risk for joint damage more effectively than conventional clinical and laboratory measures, it has the potential to complement these measures and optimise clinical decision making.

  11. Antimicrobial Properties of an Immunomodulator - 15 kDa Human Granulysin

    PubMed Central

    Wei, Hung-Mu; Lin, Li-Chih; Wang, Chiu-Feng; Lee, Yi-Jang; Chen, Yuan-Tsong; Liao, You-Di

    2016-01-01

    Granulysin, a cationic protein expressed by human natural killer cells and cytotoxic T lymphocytes, is a mediator for drug-induced Stevens-Johnson syndrome and graft-versus-host disease. Some 15 kDa granulysin are processed into 9 kDa forms and sequestered in cytolytic granules, while others are constitutively secreted into body fluids. Both 9 and 15 kDa granulysin have been shown to be a serum marker for cell-mediated immunity. Furthermore, 15 kDa is able to activate monocyte differentiation. However, its antimicrobial properties have not been clearly addressed. Here, we report a novel method to prepare both the soluble 9 and 15 kDa granulysin and show that the 15 kDa form is more effective than the 9 kDa form in exerting specific antimicrobial activity against Pseudomonas aeruginosa within a range of few micromolars. We also show that the 15 kDa granulysin is able to hyperpolarize the membrane potential and increase membrane permeability of treated bacteria. Interestingly, the bactericidal activity and membrane permeability of the granulysins were markedly reduced at lower pH (pH 5.4) as a result of probable increase in hydrophobicity of the granulysins. Additionally, we’ve also shown the granulysin to inhibit biofilm formation by P. aeruginosa. These results suggest that the 15 kDa granulysin exhibits a novel mechanism in bacteria killing in a way that’s different from most antimicrobial peptides. Our novel granulysin preparation methodology will be useful for further study of action mechanisms of other antimicrobial, cytotoxic and immunomodulating properties in granulysin-mediated diseases. PMID:27276051

  12. Therapeutic effect of infused Fluosol-DA/carbogen with ephedrine, flunarizine, or nitroprusside

    SciTech Connect

    Teicher, B.A.; Holden, S.A.; Northey, D.; Dewhirst, M.W.; Herman, T.S.

    1993-04-30

    The perfluorochemical emulsion Fluosol-DA plus carbogen breathing has been shown to increase the effectiveness of radiation therapy in preclinical solid tumors when the emulsion was administered by i.v. bolus injection. Much of the enhancement in tumor radiation response was lost when the emulsion was administered slowly. The authors hypothesized that an increase in tumor perfusion resulted when Fluosol-DA was administered rapidly. In the present study, the [alpha]/[beta] agonist ephedrine, the Ca[sup 2+] channel blocker flunarizine and the nitric oxide producing vasodilating drug nitroprusside have been tested. Ephedrine administration resulted in a decrease in the radiation plus Fluosol-DA [+-] carbogen antitumor effects in both the Lewis lung carcinoma and FSaIIC tumor systems. In contrast, flunarizine administration resulted in an increase in the efficacy of the radiation plus carbogen and the radiation plus Fluosol-DA/carbogen in both emulsion was given rapidly. Even with flunarizine administration Fluosol-DA delivered slowly was less effective than when the emulsion was given rapidly. Flunarizine with Fluosol-DA infused i.v. over 30 min followed by carbogen breathing prior to and during radiation therapy resulted in a 1.7-1.6-fold increase in response compared with 2.4-2.2-fold with Fluosol-DA administered by injection i.v. and carbogen breathing prior to and during radiation therapy using growth delay of the Lewis lung carcinoma. The effects of nitroprusside were complex. This drug had considerably more effect at 10 Gy than at higher radiation doses. These studies suggest that Fluosol-DA given by i.v. injection may increase tumor perfusion and that a drug like flunarizine may be beneficial if the Fluosol-DA is administered slowly followed by carbogen breathing and radiation therapy. 45 refs., 4 figs.

  13. Myelin management by the 18.5-kDa and 21.5-kDa classic myelin basic protein isoforms.

    PubMed

    Harauz, George; Boggs, Joan M

    2013-05-01

    The classic myelin basic protein (MBP) splice isoforms range in nominal molecular mass from 14 to 21.5 kDa, and arise from the gene in the oligodendrocyte lineage (Golli) in maturing oligodendrocytes. The 18.5-kDa isoform that predominates in adult myelin adheres the cytosolic surfaces of oligodendrocyte membranes together, and forms a two-dimensional molecular sieve restricting protein diffusion into compact myelin. However, this protein has additional roles including cytoskeletal assembly and membrane extension, binding to SH3-domains, participation in Fyn-mediated signaling pathways, sequestration of phosphoinositides, and maintenance of calcium homeostasis. Of the diverse post-translational modifications of this isoform, phosphorylation is the most dynamic, and modulates 18.5-kDa MBP's protein-membrane and protein-protein interactions, indicative of a rich repertoire of functions. In developing and mature myelin, phosphorylation can result in microdomain or even nuclear targeting of the protein, supporting the conclusion that 18.5-kDa MBP has significant roles beyond membrane adhesion. The full-length, early-developmental 21.5-kDa splice isoform is predominantly karyophilic due to a non-traditional P-Y nuclear localization signal, with effects such as promotion of oligodendrocyte proliferation. We discuss in vitro and recent in vivo evidence for multifunctionality of these classic basic proteins of myelin, and argue for a systematic evaluation of the temporal and spatial distributions of these protein isoforms, and their modified variants, during oligodendrocyte differentiation.

  14. Leonardo Da Vinci and stroke - vegetarian diet as a possible cause.

    PubMed

    Oztürk, Serefnur; Altieri, Marta; Troisi, Pina

    2010-01-01

    Leonardo da Vinci (April 15, 1452 to May 2, 1519) was an Italian Renaissance architect, musician, anatomist, inventor, engineer, sculptor, geometer, and painter. It has been gleaned from the many available historical documents that da Vinci was a vegetarian who respected and loved animals, and that he suffered from right hemiparesis in the last 5 years of his life. A vegetarian diet has both positive and negative influences on the cerebrovascular system. In this report, a possible relation between a vegetarian diet and stroke is discussed from various perspectives as related to Leonardo da Vinci's stroke.

  15. Stability of new carbapenem DA-1131 to renal dipeptidase (dehydropeptidase I).

    PubMed

    Park, Sung Wook; We, Jeoung Soon; Kim, Gye Won; Choi, Seong Hak; Park, Haeng Soon

    2002-02-01

    The stability of DA-1131 to renal dipeptidase (RDPase) (EC 3.4.13.19) was compared with that of imipenem and meropenem by V(max)/K(m) ratios as an index of the enzyme's preference for substrates. Our results showed a decreasing order of imipenem (6.24), meropenem (2.41), and DA-1131 (1.39). The biochemical evaluation of DA-1131 as the least preferred substrate of RDPase suggests its potential use as a novel beta-lactam antibiotic which may be usable without coadministration of RDPase inhibitors once its clinical suitability is proven.

  16. Augusto Celestino da Costa (1884-1956) - professor, scientist and science promoter.

    PubMed

    David-Ferreira, José-Francisco

    2009-01-01

    At the dawn of the 20th century in Lisbon, Augusto Celestino da Costa advocated a model of university practice based on research and defended the promotion of a scientific culture and the dissemination of Science in society. As a scientist, Celestino da Costa made numerous original descriptions of the cytology, histology and embryology of endocrine glands and the sympathetic nervous system. Celestino da Costa authored seminal textbooks on histology and embryology, and was responsible for launching in Portugal the first governmental agency to finance scientific activities.

  17. Arbitrary Amplitude DIA and DA Solitary Waves in Adiabatic Dusty Plasmas

    SciTech Connect

    Mamun, A. A.; Jahan, N.; Shukla, P. K.

    2008-10-15

    The dust-ion-acoustic (DIA) as well as the dust-acoustic (DA) solitary waves (SWs) in an adiabatic dusty plasma are investigated by the pseudo-potential approach which is valid for arbitrary amplitude SWs. The role of the adiabaticity of electrons and ions in modifying the basic features (polarity, speed, amplitude and width) of arbitrary amplitude DIA and DA SWs are explicitly examined. It is found that the effects of the adiabaticity of electrons and ions significantly modify the basic features (polarity, speed, amplitude and width) of the DIA and DA SWs. The implications of our results in space and laboratory dusty plasmas are briefly discussed.

  18. 2012 DA14 and Chelyabinsk: Same Day Coincidence?

    NASA Astrophysics Data System (ADS)

    Chapman, Clark R.

    2013-10-01

    A long anticipated near-miss by near-Earth asteroid (NEA) 2012 DA14 on 15 February 2013 was upstaged 16 hours earlier by the impact and atmospheric explosion of a ~20 m NEA over Chelyabinsk, Russia. DA14 was earlier estimated to be 45 m across and passage at a distance under geosynchronous satellites was considered to be a record close approach by an object of that size, a once-in-40-years event. Actual Earth impact by a 20 m NEA had been estimated to be a once-in-200-years event. A simplistic calculation gives a probability of these happening on the same day of 1-in-a-billion. Within hours of the Chelyabinsk impact, it was recognized and widely reported that the two asteroids were in very different orbits and could not, at least in any readily understandable way, be physically related to each other (e.g. fragments of the same precursor body). Also, some early reports suggested that the two asteroids had very different compositions, further undermining the possibility they were pieces of the same body. (It seems unlikely, though certainly conceivable, that the two NEAs could have different compositions yet have some kind of causal connection resulting in the same-day events.) Nevertheless, incredibly tiny probabilities beg for an explanation. Here, with the benefit of hindsight, I re-examine issues that affect the probabilities, such as size, albedo, probable composition, and numbers of NEAs of these sizes. I also consider difficult issues of framing the apparent coincidence, which dominate other uncertainties. Updated information about the physical nature of the two asteroids somewhat modifies the original estimates of probabilities. Moreover, more proper ways of framing elements of the coincidence considerably reduce the improbability of the same-day events, but not nearly to the level (e.g. 1-in-1000 or perhaps 1-in-10,000) where we could rationalize dismissing the events as “just a coincidence.” The events of 15 February 2013 deserve more sophisticated

  19. High School Student's Alternative Conceptions About the Phenomenon of the Formation of the Moon Phases. (Spanish Title: Concepciones Alternativas de Alumnos de Educación Media Sobre el Fenómeno de Formación de las Fases de La Luna.) Concepções Alternativas de Alunos do Ensino Médio Sobre o Fenômeno de Formação das Fases da Lua

    NASA Astrophysics Data System (ADS)

    Iachel, Gustavo; Langhi, Rodolfo; Fernandes Scalvi, Rosa Maria

    2008-07-01

    Forty students, at ages between 14 and 18 years old, from three schools in Bauru city, were questioned about their alternative conceptions concerning the phenomenon of formation of the Moon Phases. It was observed that some of the pupils confound the phenomenon of the formation of the Moon Phases with the phenomenon of the formation of the lunar eclipses, others are unaware of the reason of the phenomenon, they present incoherent alternative conceptions of the reality or incomplete conceptions. The results found here are aimed at the teachers of Elementary Education and can be used as a subsidy for future development of new pedagogical methods. Cuarenta estudiantes, con edad entre 14 y 18 años, pertenecientes a tres escuelas de la ciudad de Bauru, fueron cuestionados sobre sus concepciones alternativas acerca del fenómeno de la formación de las fases de la Luna. Fue observado que algunos alumnos confunden el fenómeno de formación de las fases de la Luna con el fenómeno de formación de los eclipses lunares, otros desconocen el motivo delfenómeno, presentan concepciones alternativas incoherentes con la realidad o bien presentan concepciones incompletas. Los resultados aquí encontrados son destinados a los profesores de la Enseñanza Básica y podrán ser usados como ayuda para el futuro desarrollo de nuevos métodos pedagógicos. Quarenta estudantes, com idades entre 14 e 18 anos, pertencentes a três escolas da cidade de Bauru, foram questionados sobre suas concepções alternativas acerca do fenômeno de formação das fases da Lua. Foi observado que alguns dos alunos confundem o fenômeno da formação das fases da Lua com o fenômeno da formação dos eclipses lunares, outros desconhecem o motivo do fenômeno, apresentam concepções alternativas incoerentes com a realidade ou então concepções incompletas. Os resultados aqui encontrados são destinados aos professores do Ensino Básico e poderão ser usados como subsídio parafuturo desenvolvimento de

  20. Audio annotation watermarking with robustness against DA/AD conversion

    NASA Astrophysics Data System (ADS)

    Qian, Kun; Kraetzer, Christian; Biermann, Michael; Dittmann, Jana

    2010-01-01

    In the paper we present a watermarking scheme developed to meet the specific requirements of audio annotation watermarking robust against DA/AD conversion (watermark detection after playback by loudspeaker and recording with a microphone). Additionally the described approach tries to achieve a comparably low detection complexity, so it could be embedded in the near future in low-end devices (e.g. mobile phones or other portable devices). We assume in the field of annotation watermarking that there is no specific motivation for attackers to the developed scheme. The basic idea for the watermark generation and embedding scheme is to combine traditional frequency domain spread spectrum watermarking with psychoacoustic modeling to guarantee transparency and alphabet substitution to improve the robustness. The synchronization and extraction scheme is designed to be much less computational complex than the embedder. The performance of the scheme is evaluated in the aspects of transparency, robustness, complexity and capacity. The tests reveals that 44% out of 375 tested audio files pass the simulation test for robustness, while the most appropriate category shows even 100% robustness. Additionally the introduced prototype shows an averge transparency of -1.69 in SDG, while at the same time having a capacity satisfactory to the chosen application scenario.

  1. DA WHITE DWARFS OBSERVED IN THE LAMOST PILOT SURVEY

    SciTech Connect

    Zhang Yueyang; Deng Licai; Liu Chao; Carrell, Kenneth; Yang Fan; Gao Shuang; Xu Yan; Li Jing; Zhang Haotong; Zhao Yongheng; Luo Ali; Bai Zhongrui; Yuan Hailong; Lepine, Sebastien; Newberg, Heidi Jo; Carlin, Jeffrey L.; Jin Ge

    2013-08-01

    A total of {approx}640, 000 objects from the LAMOST pilot survey have been publicly released. In this work, we present a catalog of DA white dwarfs (DAWDs) from the entire pilot survey. We outline a new algorithm for the selection of white dwarfs (WDs) by fitting Sersic profiles to the Balmer H{beta}, H{gamma}, and H{delta} lines of the spectra, and calculating the equivalent width of the Ca II K line. Two thousand nine hundred sixty-four candidates are selected by constraining the fitting parameters and the equivalent width of the Ca II K line. All the spectra of candidates are visually inspected. We identify 230 DAWDs (59 of which are already included in the Villanova and SDSS WD catalogs), 20 of which are DAWDs with non-degenerate companions. In addition, 128 candidates are classified as DAWDs/subdwarfs, which means the classifications are ambiguous. The result is consistent with the expected DAWD number estimated based on the LEGUE target selection algorithm.

  2. Voltammetric classification of ciders with PLS-DA.

    PubMed

    Górski, Łukasz; Sordoń, Wanda; Ciepiela, Filip; Kubiak, Władysław W; Jakubowska, Małgorzata

    2016-01-01

    Voltammograms recorded on the glassy carbon electrode (GCE) may be a chemical fingerprints of food samples, enabled distinguishing the origin of the considered products. In this work the objects of the study was 5 Polish ciders of various brands. For each sample 10 scans were recorded by DPV in the potential range between -0.2 and 1.0 V in Britton-Robinson buffer at pH 2.0. The signals preprocessing realized by baseline correction with 4-th degree polynomial and normalization (in 0 to 1 interval), performed to reduce problems with insufficient signal's repeatability associated with mechanical renovation of the electrode surface before each measurement. The PLS-DA classification models were built using the training set and then validated using the samples absent in the learning process. The final multi-class model with optimized complexity enables classification of the ciders with 100% sensitivity and specificity, with the exception of one cider, where specificity was 95% (for validation set). PMID:26695257

  3. GOoDA: The Generic Optimization Data Analyzer

    NASA Astrophysics Data System (ADS)

    Calafiura, P.; Eranian, S.; Levinthal, D.; Kama, S.; Vitillo, R. A.

    2012-12-01

    Modern superscalar, out-of-order microprocessors dominate large scale server computing. Monitoring their activity, during program execution, has become complicated due to the complexity of the microarchitectures and their IO interactions. Recent processors have thousands of performance monitoring events. These are required to actually provide coverage for all of the complex interactions and performance issues that can occur. Knowing which data to collect and how to interpret the results has become an unreasonable burden for code developers whose tasks are already hard enough. It becomes the task of the analysis tool developer to bridge this gap. To address this issue, a generic decomposition of how a microprocessor is using the consumed cycles allows code developers to quickly understand which of the myriad of microarchitectural complexities they are battling, without requiring a detailed knowledge of the microarchitecture. When this approach is intrinsically integrated into a performance data analysis tool, it enables software developers to take advantage of the microarchitectural methodology that has only been available to experts. The Generic Optimization Data Analyzer (GOoDA) project integrates this expertise into a profiling tool in order to lower the required expertise of the user and, being designed from the ground up with large-scale object-oriented applications in mind, it will be particularly useful for large HENP codebases

  4. [Project Leonardo-da-Vinci for better nursing care].

    PubMed

    Gábor, Katalin; Csanádi, Lajosné; Helembai, Kornélia; Szögi, Zoltánné; Tulkán, Ibolya; Unginé, Kántor Katalin

    2002-08-18

    The aim of the present paper is to inform physicians about the work completed by nurses and professors of baccalaureat nurses in the framework of Leonardo da Vinci project, organised and sponsored by the European Union. The goal of the project was to increase the effectiveness of chief nurses throughout their further training programme in the field of management. The team of Szeged chose the human resource management, since in this field is possible to achieve the greatest improvement with the smallest financial investment. We measured the fluctuations and the absentees of the nurses, the changes in the degree of education, the nurse' and patient' satisfaction at the beginning and at the end of the period studied. Except the patient's satisfaction all the other parameters improved by the end of tested period. The project provided a unique possibility to compare the state of the Hungarian nursing with that of the countries belonging to the European Union, to exchange the experience and to learn some new methods. In the framework of this project a book of two volumes was prepared containing the suggestions of EU. This book is widely available in English and in French.

  5. Near-UV absorption in very cool DA white dwarfs

    SciTech Connect

    Saumon, D.; Holberg, J. B.; Kowalski, P. M. E-mail: holberg@argus.lpl.arizona.edu

    2014-07-20

    The atmospheres of very cool, hydrogen-rich white dwarfs (WDs) (T{sub eff} < 6000 K) are challenging to model because of the increased complexity of the equation of state, chemical equilibrium, and opacity sources in a low-temperature, weakly ionized dense gas. In particular, many models that assume relatively simple models for the broadening of atomic levels and mostly ideal gas physics overestimate the flux in the blue part of their spectra. A solution to this problem that has met with some success is that additional opacity at short wavelengths comes for the extreme broadening of the Lyman α line of atomic H by collisions primarily with H{sub 2}. For the purpose of validating this model more rigorously, we acquired Hubble Space Telescope STIS spectra of eight very cool WDs (five DA and three DC stars). Combined with their known parallaxes, BVRIJHK, and Spitzer IRAC photometry, we analyze their entire spectral energy distribution (from 0.24 to 9.3 μm) with a large grid of model atmospheres and synthetic spectra. We find that the red wing of the Lyman α line reproduces the rapidly decreasing near-UV flux of these very cool stars very well. We determine better constrained values of T{sub eff} and gravity as well as upper limits to the helium abundance in their atmospheres.

  6. Biogeochemical Indicators of Paleoenvironmental Changes in a Humid Region of Amazonia (Lagoa da Pata, Sao Gabriel da Cachoeira)

    NASA Astrophysics Data System (ADS)

    Cordeiro, R. C.; Turcq, B.; Mendes, L. G.; Azeredo, J. B.; Moura, M. S.; de Oliveira, A. R.; Monteiro, F.; Santelli, R. E.

    2004-12-01

    Analysis of four sediment core collected in Lagoa da Pata lake show significant changes in the environmental history of Amazonia during the last 40,000 years. The Lagoa da Pata Lake (0°17'S, 66°40'W) is located on the top of the Morro dos Seis Lagos, an isolated hill in the extensive forested plane surface of high Rio Negro basin. The climate in the region presents mean annual precipitation around 3000 mm without dry season. Three distinct sections are clearly identified in the LPT III, LPT IV and LPT V cores. They consist of upper and lower organic-rich layers, separated by a clastic layer which represents a short period of rapid accumulation around 18 ka BP. Analysis of the organic matter composition by d13C and d15N, total Carbon and Nitrogen, sedimentary chlorophyll concentration and organic matter petrography show three different phases related to organic matter deposition. Between, at least 46,000 and 31,000 14C yr B.P., the lake presented a high water level attested by high accumulation rates of organic carbon. Values of d13C (around -32%) associated to high C/N ratio demonstrate a lignocellulosic material contribution in this phase; the second phase between the 31000 yr 14C B.P. and 18000 14C B.P. is characterised by a lower productivity attested by low sedimentary chlorophyll and total organic carbon values. The d13C values increase with medium values around -28% that may correspond to an input of organic matter rich in C4 plants (grasses). The sedimentation rate decreases significantly. High charcoal fluxes were observed in this period. At around 18000 14C yr BP, there appears to have been a sudden input of clastic material. This is represented by a sandy facies, which exhibits lower carbon contents. From 18000 14C yr B.P. to the present time occurred an increase in lacustrine productivity marked by an increase in carbon and chlorophyll derivate accumulation rates. The C/N values dropped indicting an algae organic matter contribution. It probably

  7. Thinking like Leonardo da Vinci and its implications for the modern doctor.

    PubMed

    Baum, Neil

    2013-01-01

    Most people when asked to name the most creative, innovative, and multidimensional people in history would agree that Leonardo da Vinci is either at the top or very close to the number one position on that list. Wouldn't it be nice to think like da Vinci? This article shares the seven unique principles of thinking that da Vinci used that enabled him to be the greatest painter, sculptor, architect, musician, mathematician, engineer, inventor, anatomist, geologist, cartographer, botanist, and writer of his (if not of all) time. This article will take you deep into the notebooks and codices of da Vinci, and suggest ways his ideas can be used by anyone in the healthcare profession to make them a better healthcare provider.

  8. DaG 1066: A Newfound Anomalous Ureilite with Chondritic Inclusions

    NASA Astrophysics Data System (ADS)

    Moggi Cecchi, V.; Caporali, S.; Pratesi, G.

    2015-07-01

    General description, textural, and compositional features of the polymict ureilite DaG 1066, recovered in 1999 in Libya, are provided. The meteorite contains various inclusions, among which almost pure forsterite and enstatite-bearing chondrules.

  9. Thinking like Leonardo da Vinci and its implications for the modern doctor.

    PubMed

    Baum, Neil

    2013-01-01

    Most people when asked to name the most creative, innovative, and multidimensional people in history would agree that Leonardo da Vinci is either at the top or very close to the number one position on that list. Wouldn't it be nice to think like da Vinci? This article shares the seven unique principles of thinking that da Vinci used that enabled him to be the greatest painter, sculptor, architect, musician, mathematician, engineer, inventor, anatomist, geologist, cartographer, botanist, and writer of his (if not of all) time. This article will take you deep into the notebooks and codices of da Vinci, and suggest ways his ideas can be used by anyone in the healthcare profession to make them a better healthcare provider. PMID:24228380

  10. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 3 2012-07-01 2009-07-01 true Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form 4881-4-R) EC24OC91.024...

  11. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 3 2013-07-01 2013-07-01 false Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form 4881-4-R) EC24OC91.024...

  12. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form 4881-4-R) EC24OC91.024...

  13. 32 CFR Appendix F to Part 623 - Power of Attorney (DA Form 4881-4-R)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 3 2014-07-01 2014-07-01 false Power of Attorney (DA Form 4881-4-R) F Appendix F to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. F Appendix F to Part 623—Power of Attorney (DA Form 4881-4-R) EC24OC91.024...

  14. Load evaluation of the da Vinci surgical system for transoral robotic surgery.

    PubMed

    Fujiwara, Kazunori; Fukuhara, Takahiro; Niimi, Koji; Sato, Takahiro; Kitano, Hiroya

    2015-12-01

    Transoral robotic surgery, performed with the da Vinci surgical system (da Vinci), is a surgical approach for benign and malignant lesions of the oral cavity and laryngopharynx. It provides several unique advantages, which include a 3-dimensional magnified view and ability to see and work around curves or angles. However, the current da Vinci surgical system does not provide haptic feedback. This is problematic because the potential risks specific to the transoral use of the da Vinci include tooth injury, mucosal laceration, ocular injury and mandibular fracture. To assess the potential for intraoperative injuries, we measured the load of the endoscope and the instrument of the da Vinci Si surgical system. We pressed the endoscope and instrument of the da Vinci Si against Load cell six times each and measured the dynamic load and the time-to-maximum load. We also struck the da Vinci Si endoscope and instrument against the Load cell six times each and measured the impact load. The maximum dynamic load was 7.27 ± 1.31 kg for the endoscope and 1.90 ± 0.72 for the instrument. The corresponding time-to-maximum loads were 1.72 ± 0.22 and 1.29 ± 0.34 s, but the impact loads were significantly lower than the dynamic load. It remains possible that a major load is exerted on adjacent structures by continuous contact with the endoscope and instrument of da Vinci Si. However, there is a minor delay in reaching the maximum load. Careful monitoring by an on-site assistant may, therefore, help prevent contiguous injury.

  15. OpenDA: Open Source Generic Data Assimilation Environment and its Application in Geophysical Process Models

    NASA Astrophysics Data System (ADS)

    Weerts, A.; van Velzen, N.; Verlaan, M.; Sumihar, J.; Hummel, S.; El Serafy, G.; Dhondia, J.; Gerritsen, H.; Vermeer-Ooms, S.; Loots, E.; Markus, A.; Kockx, A.

    2011-12-01

    Data Assimilation techniques are essential elements in state-of-the-art development of models and their use in operational forecasting and real-time control in the fields of groundwater, surface water and soil systems. In meteorological and atmospheric sciences, steady improvements in numerical weather forecasting and climate prediction over the last couple of decades have been enabled to a large degree by the development of community-based models and data assimilation systems. The hydrologic community should learn from the experiences of the meteorological and atmospheric communities by accelerating the transition of hydrologic DA research into operations and developing community-supported, open-source modeling and forecasting systems and data assimilation tools. In 2010, a community based open source initiative named OpenDA was started. The openDA initiative bears similarities with the well-known openMI initiative. OpenDA makes use of a set of interfaces that describe the interaction between models, observations and data assimilation algorithms. It focuses on flexible applications in portable systems for modeling geophysical processes. It provides a generic interfacing protocol that allows combination of the implemented data assimilation techniques with, in principle, any time-stepping model describing a process (atmospheric processes, 3D circulation, 2D water flow, rainfall-runoff, unsaturated flow, groundwater flow, etc.). Presently, openDA features filtering techniques and calibration techniques. The presentation will give an overview of openDA and the results of some of its practical applications.

  16. The 32 kDa Enamelin Undergoes Conformational Transitions upon Calcium Binding

    PubMed Central

    Fan, Daming; Lakshminarayanan, Rajamani; Moradian-Oldak, Janet

    2008-01-01

    The 32 kDa hydrophilic and acidic enamelin, the most stable cleavage fragment of the enamel specific glycoprotein, is believed to play vital roles in controlling crystal nucleation or growth during enamel biomineralization. Circular dichroism and Fourier transform infrared spectra demonstrate that the secondary structure of the 32 kDa enamelin has a high content of α-helix (81.5%). Quantitative analysis on the circular dichroism data revealed that the 32 kDa enamelin undergoes conformational changes with a structural preference to β-sheet as a function of calcium ions. We suggest that the increase of β-sheet conformation upon presence of Ca2+ may allow preferable interaction of the 32 kDa enamelin with apatite crystal surfaces during enamel biomineralization. The calcium association constant of the 32 kDa enamelin calculated from the fitting curve of ellipticity at 222 nm is Ka = 1.55 (±0.13) × 103 M−1, indicating a relatively low affinity. Our current biophysical studies on the 32 kDa enamelin structure provide novel insights towards understanding the enamelin-mineral interaction and subsequently the functions of enamelin during enamel formation. PMID:18508280

  17. Isolation and characterization of two plaque size variants of Theiler's murine encephalomyelitis virus (DA strain).

    PubMed

    Oleszak, E L; Leibowitz, J L; Rodriguez, M

    1988-09-01

    We have isolated two plaque size variants of Theiler's murine encephalomyelitis virus (TMEV) strain DA. One variant, TMEV-CL (CL), produced large plaques, while the other, TMEV-DS (DS), produced small plaques in L-2 cells. The DS variant yielded a lower titre in BHK cells and had a significantly slower growth rate when compared to CL and DA. In contrast, DS replicated to a higher titre in the central nervous system (CNS) of infected mice than the large plaque counterpart and DA. Furthermore, the DS (but not CL) variant was temperature-sensitive, replicating 130- to 500-fold more at 37 degrees C than at 39 degrees C. Although DS, CL and DA were able to establish persistent CNS infections in mice, only the DS variant and DA induced demyelinating disease in SJL/J mice. Therefore persistence of TMEV in the CNS is not sufficient to produce demyelinating disease. These two variants of the DA strain of TMEV will be useful for study of the viral genetic elements important in the mechanism of virus-induced demyelination.

  18. Investigation of hydrophobic organic carbon (HOC) partitioning to 1 kDa fractionated municipal wastewater colloids.

    PubMed

    McPhedran, Kerry N; Seth, Rajesh; Drouillard, Ken G

    2013-03-19

    Natural organic matter from the aquatic environment passing a 1 kDa filter has been hypothesized to not contribute appreciably to hydrophobic organic compound (HOC) partitioning; however, to our knowledge this limit has not been verified experimentally for any sorbate/sorbent system. Presently, colloidal organic carbon (COC) < 1 kDa approached 70% of the total COC (<1.5 μm) mass in primary effluent (PE) from a municipal wastewater treatment plant. Partitioning of HOCs 1,2,4,5-tetrachlorobenzene, pentachlorobenzene, and hexachlorobenzene to COC for both 1.5 μm and 1 kDa filtrates of PE was investigated using the gas-stripping technique. Contrary to the hypothesis, significant HOC-COC partitioning to the 1 kDa filtrate was observed with organic carbon-normalized partitioning coefficients (logKCOC) of 4.30, 4.36, and 3.74 for 1,2,4,5-TeCB, PeCB, and HCB, respectively. Further, partitioning to COC < 1 kDa dominated the overall partitioning of the three chlorobenzenes in the 1.5 μm filtrate, and the partitioning behavior did not follow the trend based on hydrophobicity (KOW). The results show that significant partitioning of HOC may occur to OC < 1 kDa and highlights the need for further experiments with other HOCs and COC characterization to better understand and explain the observed partitioning.

  19. In vitro degradation of the 32kDa PS II reaction centre protein

    SciTech Connect

    Eckenswiller, L.C.; Greenberg, B.M. )

    1989-04-01

    The 32kDa thylakoid membrane protein is an integral component of the PS II reaction centre. The protein, although stable in the dark, undergoes light dependent turnover. Light from the UV, visible and far-red spectral regions induce 32kDa protein degradation. To better understand 32kDa protein metabolism, an in vitro degradation system is being developed. It consists of isolated thylakoid membranes than contain radiolabelled protein. The 32kDa protein is actively and specifically degraded when the thylakoid preparation is exposed to UV or visible radiation. The protein is stable in the dark. The herbicides (atrazine and DCMU) inhibit degradation in the in vitro system as they do in vivo. Additionally, several methods of isolating thylakoids are being compared to optimize the 32kDa protein degradation reaction. The preparations will be evaluated based on their ability to permit light dependent degradation of the 32kDa protein without affecting the other membrane components.

  20. DA 5505: a novel topical formulation of terbinafine that enhances skin penetration and retention.

    PubMed

    Thapa, Raj Kumar; Han, Sang-Duk; Park, Hyoung Geun; Son, Miwon; Jun, Joon Ho; Kim, Jong Oh

    2015-01-01

    Topical fungal infections can become severe if left untreated. Efficient treatment modalities for topical fungal infections aid the penetration of antifungal agents deep into viable skin layers. Terbinafine is a fungicidal agent that inhibits ergosterol, an essential fungal component. The main objective of this study was to evaluate skin permeation and retention of a terbinafine-loaded solution containing chitosan as a film former. Comparative assessment of skin permeation and retention was performed using a prepared formulation (DA 5505) and marketed formulations of terbinafine in murine and porcine skin. To mimic fungal infection of skin, keratinized skin was induced in NC/Nga mice. In comparison with the marketed formulations, DA 5505 exhibited significantly better skin permeation. The flux, permeation coefficient, and enhancement ratio of terbinafine were remarkably increased by DA 5505 in comparison with the marketed formulations, and lag time was dramatically reduced. DA 5505 significantly increased cumulative terbinafine retention in viable skin layers in comparison with the marketed solution, suggesting enhanced efficacy. Furthermore, DA 5505 exhibited superior skin permeation in normal skin and keratinized skin. Thus, the DA 5505 formulation has the potential to effectively deliver terbinafine to superficial and deep cutaneous fungal infections.

  1. The sandfly fauna (Diptera: Psychodidae: Phlebotominae) of the Parque Estadual da Serra da Tiririca, Rio de Janeiro, Brazil

    PubMed Central

    Rodrigues, Andressa Alencastre Fuzari; Barbosa, Vanessa de Araújo; Andrade, José Dilermando; Brazil, Reginaldo Peçanha

    2013-01-01

    Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can be characterised as a peridomestic transmission that occurs in modified natural environments. The aim of this work was to study the fauna and ecological characteristics of sandflies in an environmentally protected area (the State Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the municipalities of Niterói and Maricá and their possible relationship with leishmaniasis. Captures were performed using light traps during the night once a month for one year in both sylvatic environments and areas surrounding homes near the park. A total of 1,037 sandflies were captured, belonging to nine genera and 12 species: Evandromyia tupynambai (34.1%), Migonemyia migonei (20.6%), Brumptomyia cunhai (13.8%), Micropygomyia schreiberi (9.7%), Psathyromyia lanei (6.5%), Brumptomyia nitzulescui (5.7%), Evandromyia edwardsi (5.4%), Nyssomyia intermedia (2.8%), Evandromyia cortelezzii (0.6%), Pintomyia bianchigalatiae (0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia microps (0.1%). Both Mg. migonei and Ny. intermedia may be acting as vectors of CL in this area. PMID:24141956

  2. The sandfly fauna (Diptera: Psychodidae: Phlebotominae) of the Parque Estadual da Serra da Tiririca, Rio de Janeiro, Brazil.

    PubMed

    Rodrigues, Andressa Alencastre Fuzari; Barbosa, Vanessa de Araújo; Andrade Filho, José Dilermando; Brazil, Reginaldo Peçanha

    2013-11-01

    Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can be characterised as a peridomestic transmission that occurs in modified natural environments. The aim of this work was to study the fauna and ecological characteristics of sandflies in an environmentally protected area (the State Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the municipalities of Niterói and Maricá and their possible relationship with leishmaniasis. Captures were performed using light traps during the night once a month for one year in both sylvatic environments and areas surrounding homes near the park. A total of 1,037 sandflies were captured, belonging to nine genera and 12 species: Evandromyia tupynambai (34.1%), Migonemyia migonei (20.6%), Brumptomyia cunhai (13.8%), Micropygomyia schreiberi (9.7%), Psathyromyia lanei (6.5%), Brumptomyia nitzulescui (5.7%), Evandromyia edwardsi (5.4%), Nyssomyia intermedia (2.8%), Evandromyia cortelezzii (0.6%), Pintomyia bianchigalatiae (0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia microps (0.1%). Both Mg. migonei and Ny. intermedia may be acting as vectors of CL in this area.

  3. Antiapoptotic activity of 30 kDa lipoproteins family from fat body tissue of silkworm, Bombyx mori.

    PubMed

    Pakkianathan, Britto Cathrin; Singh, Nitin Kumar; König, Simone; Krishnan, Muthukalingan

    2015-10-01

    The family of 30 kDa lipoproteins (LP1-5) is abundant in silkworm pupa fat body (FB) and hemolymph. One of its members, the 29 kDa protein decreased in concentration from peripheral (PP) FB tissue but was sustained in perivisceral (PV) FB tissue at the time of apoptosis. This study investigated the correlation of the 30 kDa proteins with FB apoptosis. Two protein fractions were purified, a 29 and a 30/31 kDa protein fraction, and they were used to test for activity against actinomycin D-induced apoptosis in the FB tissues. Concentrations as little as 50 μg/mL of the 29 kDa protein fraction efficiently inhibited apoptosis. Less antiapoptotic activity was detected for the higher MW fraction; DNA fragmentation was observed in FB tissue treated with 50 μg/mL of the 30/31 kDa fraction. The viability of the cells in the 29 kDa protein-supplemented culture was 40% higher than in the 31 kDa protein-supplemented culture. However, the 30 kDa lipoproteins were not able to prevent scheduled FB degeneration during silkworm metamorphosis. Thus, it is hypothesized that the antiapoptotic 29 kDa protein needs to be proteolytically degraded by a regulatory mechanism to allow programmed cell death of FB tissue.

  4. Leonardo da Vinci and the first hemodynamic observations.

    PubMed

    Martins e Silva, J

    2008-02-01

    Leonardo da Vinci was a genius whose accomplishments and ideas come down to us today, five centuries later, with the freshness of innovation and the fascination of discovery. This brief review begins with a summary of Leonardo's life and a description of the most important works of art that he bequeathed us, and then concentrates on his last great challenge. There was a point at which Leonardo's passion for art gave way to the study of human anatomy, not only to improve his drawing but to go beyond what had been simply a representation of form to understand the underlying functioning. Among his many interests, we focus on his study of the heart and blood vessels, which he observed carefully in animals and human autopsies, and reproduced in drawings of great quality with annotations of astonishing acuteness. The experience that he had acquired from observing the flow of water in currents and around obstacles, and the conclusions that he drew concerning hydrodynamics, were central to his interpretation of the mechanisms of the heart and of blood flow, to which he devoted much of his time between 1508 and 1513. From these studies, immortalized in drawings of great clarity, come what are acknowledged to be the first hemodynamic records, in which Leonardo demonstrates the characteristics of blood flow in the aorta and great vessels and the importance of blood reflux and the formation of eddies in the sinus in aortic valve his assiduous and careful observations, and his subsequent deductions, Leonardo put forward detailed findings on hemodynamic questions that advanced technology has only recently enabled us to confirm.

  5. An Introduction to the DA-T Gibbs Sampler for the Two-Parameter Logistic (2PL) Model and beyond

    ERIC Educational Resources Information Center

    Maris, Gunter; Bechger, Timo M.

    2005-01-01

    The DA-T Gibbs sampler is proposed by Maris and Maris (2002) as a Bayesian estimation method for a wide variety of "Item Response Theory (IRT) models". The present paper provides an expository account of the DA-T Gibbs sampler for the 2PL model. However, the scope is not limited to the 2PL model. It is demonstrated how the DA-T Gibbs sampler for…

  6. 82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.

    PubMed

    Winick-Ng, Warren; Caetano, Fabiana A; Winick-Ng, Jennifer; Morey, Trevor M; Heit, Bryan; Rylett, R Jane

    2016-01-01

    The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1-42 (Aβ1-42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1-42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generation sequencing (ChIP-seq), we identified that Aβ1-42-exposure increases 82-kDa ChAT association with gene promoters and introns. The Aβ1-42-induced 82-kDa ChAT aggregates co-localize with special AT-rich binding protein 1 (SATB1), which anchors DNA to scaffolding/matrix attachment regions (S/MARs). SATB1 had a similar genomic association as 82-kDa ChAT, with both proteins associating with synapse and cell stress genes. After Aβ1-42 -exposure, both SATB1 and 82-kDa ChAT are enriched at the same S/MAR on the APP gene, with 82-kDa ChAT expression attenuating an increase in an isoform-specific APP mRNA transcript. Finally, 82-kDa ChAT and SATB1 have patterned genomic association at regions enriched with S/MAR binding motifs. These results demonstrate that 82-kDa ChAT and SATB1 play critical roles in the response of neural cells to acute Aβ-exposure. PMID:27052102

  7. 82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions

    PubMed Central

    Winick-Ng, Warren; Caetano, Fabiana A.; Winick-Ng, Jennifer; Morey, Trevor M.; Heit, Bryan; Rylett, R. Jane

    2016-01-01

    The M-transcript of human choline acetyltransferase (ChAT) produces an 82-kDa protein (82-kDa ChAT) that concentrates in nuclei of cholinergic neurons. We assessed the effects of acute exposure to oligomeric amyloid-β1–42 (Aβ1–42) on 82-kDa ChAT disposition in SH-SY5Y neural cells, finding that acute exposure to Aβ1–42 results in increased association of 82-kDa ChAT with chromatin and formation of 82-kDa ChAT aggregates in nuclei. When measured by chromatin immunoprecipitation with next-generation sequencing (ChIP-seq), we identified that Aβ1–42 -exposure increases 82-kDa ChAT association with gene promoters and introns. The Aβ1–42 -induced 82-kDa ChAT aggregates co-localize with special AT-rich binding protein 1 (SATB1), which anchors DNA to scaffolding/matrix attachment regions (S/MARs). SATB1 had a similar genomic association as 82-kDa ChAT, with both proteins associating with synapse and cell stress genes. After Aβ1–42 -exposure, both SATB1 and 82-kDa ChAT are enriched at the same S/MAR on the APP gene, with 82-kDa ChAT expression attenuating an increase in an isoform-specific APP mRNA transcript. Finally, 82-kDa ChAT and SATB1 have patterned genomic association at regions enriched with S/MAR binding motifs. These results demonstrate that 82-kDa ChAT and SATB1 play critical roles in the response of neural cells to acute Aβ -exposure. PMID:27052102

  8. High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster

    PubMed Central

    2014-01-01

    Background The Drosophila melanogaster Serpin 42 Da gene (previously Serpin 4) encodes a serine protease inhibitor that is capable of remarkable functional diversity through the alternative splicing of four different reactive centre loop exons. Eight protein isoforms of Serpin 42 Da have been identified to date, targeting the protease inhibitor to both different proteases and cellular locations. Biochemical and genetic studies suggest that Serpin 42 Da inhibits target proteases through the classical serpin ‘suicide’ inhibition mechanism, however the crystal structure of a representative Serpin 42 Da isoform remains to be determined. Results We report two high-resolution crystal structures of Serpin 42 Da representing the A/B isoforms in the cleaved conformation, belonging to two different space-groups and diffracting to 1.7 Å and 1.8 Å. Structural analysis reveals the archetypal serpin fold, with the major elements of secondary structure displaying significant homology to the vertebrate serpin, neuroserpin. Key residues known to have central roles in the serpin inhibitory mechanism are conserved in both the hinge and shutter regions of Serpin 42 Da. Furthermore, these structures identify important conserved interactions that appear to be of crucial importance in allowing the Serpin 42 Da fold to act as a versatile template for multiple reactive centre loops that have different sequences and protease specificities. Conclusions In combination with previous biochemical and genetic studies, these structures confirm for the first time that the Serpin 42 Da isoforms are typical inhibitory serpin family members with the conserved serpin fold and inhibitory mechanism. Additionally, these data reveal the remarkable structural plasticity of serpins, whereby the basic fold is harnessed as a template for inhibition of a large spectrum of proteases by reactive centre loop exon ‘switching’. This is the first structure of a Drosophila serpin reported to date

  9. The rare DAT coding variant Val559 perturbs DA neuron function, changes behavior, and alters in vivo responses to psychostimulants.

    PubMed

    Mergy, Marc A; Gowrishankar, Raajaram; Gresch, Paul J; Gantz, Stephanie C; Williams, John; Davis, Gwynne L; Wheeler, C Austin; Stanwood, Gregg D; Hahn, Maureen K; Blakely, Randy D

    2014-11-01

    Despite the critical role of the presynaptic dopamine (DA) transporter (DAT, SLC6A3) in DA clearance and psychostimulant responses, evidence that DAT dysfunction supports risk for mental illness is indirect. Recently, we identified a rare, nonsynonymous Slc6a3 variant that produces the DAT substitution Ala559Val in two male siblings who share a diagnosis of attention-deficit hyperactivity disorder (ADHD), with other studies identifying the variant in subjects with bipolar disorder (BPD) and autism spectrum disorder (ASD). Previously, using transfected cell studies, we observed that although DAT Val559 displays normal total and surface DAT protein levels, and normal DA recognition and uptake, the variant transporter exhibits anomalous DA efflux (ADE) and lacks capacity for amphetamine (AMPH)-stimulated DA release. To pursue the significance of these findings in vivo, we engineered DAT Val559 knock-in mice, and here we demonstrate in this model the presence of elevated extracellular DA levels, altered somatodendritic and presynaptic D2 DA receptor (D2R) function, a blunted ability of DA terminals to support depolarization and AMPH-evoked DA release, and disruptions in basal and psychostimulant-evoked locomotor behavior. Together, our studies demonstrate an in vivo functional impact of the DAT Val559 variant, providing support for the ability of DAT dysfunction to impact risk for mental illness.

  10. Localization, biosynthesis, processing and isolation of a major 126 kDa antigen of the parasitophorous vacuole of Plasmodium falciparum.

    PubMed

    Delplace, P; Fortier, B; Tronchin, G; Dubremetz, J F; Vernes, A

    1987-04-01

    Monoclonal antibodies prepared against a 50 kDa antigen found in Plasmodium falciparum culture supernatants identify a 126 kDa polypeptide which can be localized by immunofluorescence and immunoelectronmicroscopy at the periphery of the schizonts. This polypeptide is released from the infected erythrocytes by mild saponin lysis and is probably a component of the parasitophorous vacuole. Pulse chase kinetic analysis demonstrated its disappearance from the parasitized red blood cell from 6 to 10 h after being synthesized and the concomitant appearance of the 50 kDa molecule in the culture supernatant. Purification of metabolically labeled, schizont infected cells demonstrated that spontaneous release of merozoites is needed for the processing of the 126 to the 50 kDa whereas reinvasion is not. Polyclonal antibodies were raised in rabbit against affinity purified 126 kDa protein. These antibodies, together with another 126 kDa specific monoclonal antibody have enabled us to characterize two other cleavage products of the 126 kDa antigen in culture supernatants, namely 47 and 18 kDa polypeptides. We believe that the processing of the 126 kDa protein into low molecular weight fragments reflects a proteolytic event which may participate in merozoite release.

  11. Low strain, long life creep fatigue of AF2-1DA and INCO 718

    NASA Technical Reports Server (NTRS)

    Thakker, A. B.; Cowles, B. A.

    1983-01-01

    Two aircraft turbine disk alloys, GATORIZED AF2-DA and INCO 718 were evaluated for their low strain long life creep-fatigue behavior. Static (tensile and creep rupture) and cyclic properties of both alloys were characterized. The cntrolled strain LCF tests were conducted at 760 C (1400 F) and 649 C (1200 F) for AF2-1DA and INCO 718, respectively. Hold times were varied for tensile, compressive and tensile/compressive strain dwell (relaxation) tests. Stress (creep) hold behavior of AF2-1DA was also evaluated. Generally, INCO 718 exhibited more pronounced reduction in cyclic life due to hold than AF2-1DA. The percent reduction in life for both alloys for strain dwell tests was greater at low strain ranges (longer life regime). Changing hold time from 0 to 0.5, 2.0 and 15.0 min. resulted in corresponding reductions in life. The continuous cycle and cyclic/dwell initiation failure mechanism was predominantly transgranular for AF2-1DA and intergranular for INCO 718.

  12. Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes

    PubMed Central

    Park, Young-Jin; Park, Jeong-Hyun; Chai, Jong-Yil

    2014-01-01

    This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species. PMID:24850966

  13. Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis

    PubMed Central

    Song, Hyun-Ouk

    2016-01-01

    Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis. PMID:26951982

  14. Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis.

    PubMed

    Song, Hyun-Ouk

    2016-02-01

    Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis. PMID:26951982

  15. In vitro study on the interaction between the 32 kDa enamelin and amelogenin

    PubMed Central

    Fan, Daming; Du, Chang; Sun, Zhi; Lakshminarayanan, Rajamani; Moradian-Oldak, Janet

    2015-01-01

    Enamel extracelluar matrix components play vital roles in controlling crystal nucleation and growth during enamel formation. We investigated the interaction between the 32 kDa enamelin fragment and amelogenin using immunochemical and biophysical methods. Immunoprecipitation studies revealed that the 32 kDa enamelin and amelogenin eluted together from a Protein A column. Dynamic light scattering results showed that the 32 kDa enamelin had a profound effect on amelogenin assembly at pH 8.0, causing partial dissociation of the nanospheres, in a dose-dependent manner. The appearance of an isodichroic point and the shifting and intensity decrease of the ellipticity minima in the circular dichroism spectra of amelogenin following the addition of the 32 kDa enamelin were indicative of conformational changes in amelogenin and of a direct interaction between the two macromolecules. Our results collectively demonstrate that the 32 kDa enamelin has a direct interaction with amelogenin in vitro. Our current studies provide novel insights into understanding possible cooperation between enamelin and amelogenin in macromolecular self-assembly and in controlling enamel mineral formation. PMID:19263522

  16. Alternative translation initiation site in the DA strain of Theiler's murine encephalomyelitis virus.

    PubMed Central

    Kong, W P; Roos, R P

    1991-01-01

    Polyprotein processing studies of Theiler's murine encephalomyelitis virus (TMEV), a group of mouse picornaviruses, demonstrated synthesis of a protein we have called l during in vitro translations from the RNA of DA, a demyelinating strain of TMEV, but not GDVII, an acute neurovirulent strain. We have proposed that l is synthesized from an alternative initiation site in the DA leader (L) coding area out of phase with the polyprotein reading frame (R. P. Roos, W.-P. Kong, B. L. Semler, J. Virol. 63:5344-5353, 1989). We now provide support for this proposal from experiments involving in vitro translation of three separate mutations of an infectious DA cDNA clone: DA"l"-1, which contains a base mismatch at the putative initiation codon of l, DAL-1, which contains a base mismatch at the presumed authentic initiation site of L at the beginning of the polyprotein; and DAL:NheI, which contains nucleotides coding for a four-amino-acid insertion in the L coding area with a termination codon in the l reading frame. Our results demonstrate that the DA strain uses an alternative initiation site and reading frame to in vitro synthesize l. l may have a role in the biological activity of the virus. Images PMID:2033677

  17. Audio watermarking robust against D/A and A/D conversions

    NASA Astrophysics Data System (ADS)

    Xiang, Shijun

    2011-12-01

    Digital audio watermarking robust against digital-to-analog (D/A) and analog-to-digital (A/D) conversions is an important issue. In a number of watermark application scenarios, D/A and A/D conversions are involved. In this article, we first investigate the degradation due to DA/AD conversions via sound cards, which can be decomposed into volume change, additional noise, and time-scale modification (TSM). Then, we propose a solution for DA/AD conversions by considering the effect of the volume change, additional noise and TSM. For the volume change, we introduce relation-based watermarking method by modifying groups of the energy relation of three adjacent DWT coefficient sections. For the additional noise, we pick up the lowest-frequency coefficients for watermarking. For the TSM, the synchronization technique (with synchronization codes and an interpolation processing operation) is exploited. Simulation tests show the proposed audio watermarking algorithm provides a satisfactory performance to DA/AD conversions and those common audio processing manipulations.

  18. Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron

    PubMed Central

    Yu, Hung-Hsiang

    2016-01-01

    In the Drosophila olfactory system, odorant information is sensed by olfactory sensory neurons and relayed from the primary olfactory center, the antennal lobe (AL), to higher olfactory centers via olfactory projection neurons (PNs). A major portion of the AL is constituted with dendrites of four groups of PNs, anterodorsal PNs (adPNs), lateral PNs (lPNs), lateroventral PNs (lvPNs) and ventral PNs (vPNs). Previous studies have been focused on the development and function of adPNs and lPNs, while the investigation on those of lvPNs and vPNs received less attention. Here, we study the molecular and cellular mechanisms underlying the morphogenesis of a putative male-pheromone responding vPN, the DA1 vPN. Using an intersection strategy to remove background neurons labeled within a DA1 vPN-containing GAL4 line, we depicted morphological changes of the DA1 vPN that occurs at the pupal stage. We then conducted a pilot screen using RNA interference knock-down approach to identify cell surface molecules, including Down syndrome cell adhesion molecule 1 and Semaphorin-1a, that might play essential roles for the DA1 vPN morphogenesis. Taken together, by revealing molecular and cellular basis of the DA1 vPN morphogenesis, we should provide insights into future comprehension of how vPNs are assembled into the olfactory neural circuitry. PMID:27163287

  19. Morphogenetic Studies of the Drosophila DA1 Ventral Olfactory Projection Neuron.

    PubMed

    Shen, Hung-Chang; Wei, Jia-Yi; Chu, Sao-Yu; Chung, Pei-Chi; Hsu, Tsai-Chi; Yu, Hung-Hsiang

    2016-01-01

    In the Drosophila olfactory system, odorant information is sensed by olfactory sensory neurons and relayed from the primary olfactory center, the antennal lobe (AL), to higher olfactory centers via olfactory projection neurons (PNs). A major portion of the AL is constituted with dendrites of four groups of PNs, anterodorsal PNs (adPNs), lateral PNs (lPNs), lateroventral PNs (lvPNs) and ventral PNs (vPNs). Previous studies have been focused on the development and function of adPNs and lPNs, while the investigation on those of lvPNs and vPNs received less attention. Here, we study the molecular and cellular mechanisms underlying the morphogenesis of a putative male-pheromone responding vPN, the DA1 vPN. Using an intersection strategy to remove background neurons labeled within a DA1 vPN-containing GAL4 line, we depicted morphological changes of the DA1 vPN that occurs at the pupal stage. We then conducted a pilot screen using RNA interference knock-down approach to identify cell surface molecules, including Down syndrome cell adhesion molecule 1 and Semaphorin-1a, that might play essential roles for the DA1 vPN morphogenesis. Taken together, by revealing molecular and cellular basis of the DA1 vPN morphogenesis, we should provide insights into future comprehension of how vPNs are assembled into the olfactory neural circuitry.

  20. Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase

    SciTech Connect

    Shoseyov, O.; Doi, R.H. )

    1990-03-01

    The cellulase complex from Clostridium cellulovorans has been purified and its subunit composition determined. The complex exhibits cellulase activity against crystalline cellulose as well as carboxymethylcellulase (CMCase) and cellobiohydrolase activities. Three major subunits are present with molecular masses of 170, 100, and 70 kDa. The 100-kDa subunit is the major CMCase, although at least four other, minor subunits show CMCase activity. The 170-kDa subunit has the highest affinity for cellulose, does not have detectable enzymatic activity, but is necessary for cellulase activity. Immunological studies indicate that the 170-kDa subunit is not required for binding of the catalytic subunits to cellulose and therefore does not function solely as an anchor protein. Thus this core subunit must have multiple functions. The authors propose a working hypothesis that the binding of the 170-kDa subunit converts the crystalline cellulose to a form that is capable of being hydrolyzed in a cooperative fashion by the associated catalytic subunits.

  1. PanDA: Exascale Federation of Resources for the ATLAS Experiment at the LHC

    NASA Astrophysics Data System (ADS)

    Barreiro Megino, Fernando; Caballero Bejar, Jose; De, Kaushik; Hover, John; Klimentov, Alexei; Maeno, Tadashi; Nilsson, Paul; Oleynik, Danila; Padolski, Siarhei; Panitkin, Sergey; Petrosyan, Artem; Wenaus, Torre

    2016-02-01

    After a scheduled maintenance and upgrade period, the world's largest and most powerful machine - the Large Hadron Collider(LHC) - is about to enter its second run at unprecedented energies. In order to exploit the scientific potential of the machine, the experiments at the LHC face computational challenges with enormous data volumes that need to be analysed by thousand of physics users and compared to simulated data. Given diverse funding constraints, the computational resources for the LHC have been deployed in a worldwide mesh of data centres, connected to each other through Grid technologies. The PanDA (Production and Distributed Analysis) system was developed in 2005 for the ATLAS experiment on top of this heterogeneous infrastructure to seamlessly integrate the computational resources and give the users the feeling of a unique system. Since its origins, PanDA has evolved together with upcoming computing paradigms in and outside HEP, such as changes in the networking model, Cloud Computing and HPC. It is currently running steadily up to 200 thousand simultaneous cores (limited by the available resources for ATLAS), up to two million aggregated jobs per day and processes over an exabyte of data per year. The success of PanDA in ATLAS is triggering the widespread adoption and testing by other experiments. In this contribution we will give an overview of the PanDA components and focus on the new features and upcoming challenges that are relevant to the next decade of distributed computing workload management using PanDA.

  2. Low strain, long life creep fatigue of AF2-1DA and INCO 718

    SciTech Connect

    Thakker, A.B.; Cowles, B.A.

    1983-04-01

    Two aircraft turbine disk alloys, GATORIZED AF2-DA and INCO 718 were evaluated for their low strain long life creep-fatigue behavior. Static (tensile and creep rupture) and cyclic properties of both alloys were characterized. The cntrolled strain LCF tests were conducted at 760 C (1400 F) and 649 C (1200 F) for AF2-1DA and INCO 718, respectively. Hold times were varied for tensile, compressive and tensile/compressive strain dwell (relaxation) tests. Stress (creep) hold behavior of AF2-1DA was also evaluated. Generally, INCO 718 exhibited more pronounced reduction in cyclic life due to hold than AF2-1DA. The percent reduction in life for both alloys for strain dwell tests was greater at low strain ranges (longer life regime). Changing hold time from 0 to 0.5, 2.0 and 15.0 min. resulted in corresponding reductions in life. The continuous cycle and cyclic/dwell initiation failure mechanism was predominantly transgranular for AF2-1DA and intergranular for INCO 718.

  3. Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase.

    PubMed Central

    Shoseyov, O; Doi, R H

    1990-01-01

    The cellulase complex from Clostridium cellulovorans has been purified and its subunit composition determined. The complex exhibits cellulase activity against crystalline cellulose as well as carboxymethylcellulase (CMCase) and cellobiohydrolase activities. Three major subunits are present with molecular masses of 170, 100, and 70 kDa. The 100-kDa subunit is the major CMCase, although at least four other, minor subunits show CMCase activity. The 170-kDa subunit has the highest affinity for cellulose, does not have detectable enzymatic activity, but is necessary for cellulase activity. Immunological studies indicate that the 170-kDa subunit is not required for binding of the catalytic subunits to cellulose and therefore does not function solely as an anchor protein. Thus this core subunit must have multiple functions. We propose a working hypothesis that the binding of the 170-kDa subunit converts the crystalline cellulose to a form that is capable of being hydrolyzed in a cooperative fashion by the associated catalytic subunits. Images PMID:2107547

  4. Looking for the Perfect Mentor.

    PubMed

    Sá, Ana Pinheiro; Teixeira-Pinto, Cristina; Veríssimo, Rafaela; Vilas-Boas, Andreia; Firmino-Machado, João

    2015-01-01

    Introdução: A formação médica pós-graduada consiste num processo complexo no qual os orientadores assumem um papel fundamental. Apesar da sua importância, pouco se sabe sobre os orientadores dos internos portugueses. O presente estudo pretende caracterizar os orientadores de formação em Medicina Interna em Portugal. Material e Métodos: Realizado um estudo observacional, transversal e de carácter analítico, através um questionário online anónimo onde foram avaliadas as características demográficas dos orientadores, o seu percurso na Medicina Interna e envolvimento com o processo formativo. Resultados: Dos 213 questionários válidos (taxa de resposta estimada de 28,4%), a média global da satisfação com o orientador encontra-se nos 4,52 pontos (± 1,33), sendo a relação entre interno e orientador classificada nos 4,86 ± 1,04 pontos. O orientador âidealâfoi caracterizado como dedicado e responsável (4,9 ± 1,37 pontos), com domínio de competências práticas (4,8 ± 1,12 pontos) e teóricas (4,8 ± 1,07 pontos). Foram identificados como preditores da satisfação dos internos com o orientador a relação estabelecidaentre ambos, o envolvimento do orientador na formação, o seu dinamismo, inovação e disponibilidade [modelo explicativo de 82,5% da satisfação (R2 = 0,83; R2 a = 0,82)]. Discussão: O orientador desempenha um papel preponderante no sucesso da formação pós-graduada. Os internos de Medicina Interna em Portugal encontram-se globalmente satisfeitos com os seus orientadores e valorizam preferencialmente as suas capacidades pedagógicas. Conclusão: Este estudo aponta para a importância do orientador de formação na satisfação do interno com o seu internato, alertando para a necessidade de investir nos orientadores como forma de investimento na formação médica pós-graduada.

  5. gLExec Integration with the ATLAS PanDA Workload Management System

    NASA Astrophysics Data System (ADS)

    Karavakis, E.; Barreiro, F.; Campana, S.; De, K.; Di Girolamo, A.; Litmaath, M.; Maeno, T.; Medrano, R.; Nilsson, P.; Wenaus, T.

    2015-12-01

    ATLAS user jobs are executed on Worker Nodes (WNs) by pilots sent to sites by pilot factories. This paradigm serves to allow a high job reliability and although it has clear advantages, such as making the working environment homogeneous, the approach presents security and traceability challenges. To address these challenges, gLExec can be used to let the payloads for each user be executed under a different UNIX user id that uniquely identifies the ATLAS user. This paper describes the recent improvements and evolution of the security model within the ATLAS PanDA system, including improvements in the PanDA pilot, in the PanDA server and their integration with MyProxy, a credential caching system that entitles a person or a service to act in the name of the issuer of the credential. Finally, it presents results from ATLAS user jobs running with gLExec and describes the deployment campaign within ATLAS.

  6. Detection of trace helium in G104 - 27, a 26,000 K DA white dwarf

    SciTech Connect

    Holberg, J.B.; Kidder, K.M.; Wesemael, F. Montreal Universite, Montreal )

    1990-12-01

    The detection of the He I 4471 A feature in the moderately hot DA white dwarf G104 - 27 places this star within the rather sparse class of DAB white dwarfs. It does not appear possible, on the basis of the present data, to distinguish between a stratified and a homogeneously mixed atmosphere. While a stratified structure would be natural for a DA white dwarf, G104 - 27 resides near the red edge of the DB gap, in a region where DA stars could transform into DB stars through convective mixing in the underlying He envelope and thereby destroy envelope stratification. Both atmospheres are consistent with the existing soft X-ray flux limit for this star. 37 refs.

  7. Fluorescence spectroscopy coupled with PARAFAC and PLS DA for characterization and classification of honey.

    PubMed

    Lenhardt, Lea; Bro, Rasmus; Zeković, Ivana; Dramićanin, Tatjana; Dramićanin, Miroslav D

    2015-05-15

    Fluorescence spectroscopy coupled with parallel factor analysis (PARAFAC) and Partial least squares Discriminant Analysis (PLS DA) were used for characterization and classification of honey. Excitation emission spectra were obtained for 95 honey samples of different botanical origin (acacia, sunflower, linden, meadow, and fake honey) by recording emission from 270 to 640 nm with excitation in the range of 240-500 nm. The number of fluorophores present in honey, excitation and emission spectra of each fluorophore, and their relative concentration are determined using a six-component PARAFAC model. Emissions from phenolic compounds and Maillard reaction products exhibited the largest difference among classes of honey of different botanical origin. The PLS DA classification model, constructed from PARAFAC model scores, detected fake honey samples with 100% sensitivity and specificity. Honey samples were also classified using PLS DA with errors of 0.5% for linden, 10% for acacia, and about 20% for both sunflower and meadow mix.

  8. The coolest DA white dwarfs detected at soft X-ray wavelengths

    NASA Technical Reports Server (NTRS)

    Kidder, K. M.; Holberg, J. B.; Barstow, M. A.; Tweedy, R. W.; Wesemael, F.

    1992-01-01

    New soft X-ray/EUV photometric observations of the DA white dwarfs KPD 0631 + 1043 = WD 0631 + 107 and PG 1113 + 413 = WD 1113 + 413 are analyzed. Previously reported soft X-ray detections of three other DAs and the failure to detect a fourth DA in deep Exosat observations are investigated. New ground-based spectra are presented for all of the objects, with IUE Ly-alpha spectra for some. These data are used to constrain the effective temperatures and surface gravities. The improved estimates of these parameters are employed to refer a photospheric He abundance for the hotter objects and to elucidate an effective observational low-temperature threshold for the detection of pure hydrogen DA white dwarfs at soft X-ray wavelengths.

  9. Detection of trace helium in G104 - 27, a 26,000 K DA white dwarf

    NASA Technical Reports Server (NTRS)

    Holberg, J. B.; Kidder, K. M.; Wesemael, F.

    1990-01-01

    The detection of the He I 4471 A feature in the moderately hot DA white dwarf G104 - 27 places this star within the rather sparse class of DAB white dwarfs. It does not appear possible, on the basis of the present data, to distinguish between a stratified and a homogeneously mixed atmosphere. While a stratified structure would be natural for a DA white dwarf, G104 - 27 resides near the red edge of the DB gap, in a region where DA stars could transform into DB stars through convective mixing in the underlying He envelope and thereby destroy envelope stratification. Both atmospheres are consistent with the existing soft X-ray flux limit for this star.

  10. Characterization of the 70 kDa polypeptide of the Na/Ca exchanger.

    PubMed Central

    Saba, R I; Bollen, A; Herchuelz, A

    1999-01-01

    The Na/Ca exchanger is associated with 160, 120 and 70 kDa polypeptides whose nature is poorly understood. We have purified and characterized the Na/Ca exchanger from bovine cardiac sarcolemmal vesicles (SLVs) by using ion-exchange and affinity chromatographies. The Na/Ca exchanger-enriched fraction was reconstituted into asolectin liposomes [lipid to protein ratio 10:1 (w/w)] that showed Na/Ca exchange activity. Under non-reducing conditions, SDS/PAGE showed a single 70 kDa polypeptide, which was further characterized by immunoblots with different antibodies: SWant, raised against the purified exchanger protein; NH2-terminus, residues 1-21; NCX1, residues 393-406; and Exon F, residues 622-644. Immunoblots under reducing conditions with SWant, NH2-terminus and NCX1 showed three bands migrating at 160, 120 and 70 kDa for SLV preparations, whereas Exon F reacted only with the 160 and 120 kDa bands. Under non-reducing conditions, immunoblots with purified reconstituted Na/Ca exchanger showed a single band at 70 kDa reacting with SWant, NH2-terminus and NCX1 but not with Exon F. We conclude that the 70 kDa protein is associated with Na/Ca exchange activity, has the same N-terminal sequence as the cloned bovine cardiac exchanger, and has its length decreased by at least 35% from its C-terminal portion as compared with that of the wild-type exchanger. PMID:9931309

  11. Recurring X-ray outbursts in the supernova impostor SN 2010da in NGC 300

    NASA Astrophysics Data System (ADS)

    Binder, B.; Williams, B. F.; Kong, A. K. H.; Gaetz, T. J.; Plucinsky, P. P.; Skillman, E. D.; Dolphin, A.

    2016-04-01

    We present new observations of the `supernova impostor' SN 2010da using the Chandra X-ray Observatory and the Hubble Space Telescope. During the initial 2010 outburst, the 0.3-10 keV luminosity was observed by Swift to be ˜5 × 1038 erg s-1 and faded by a factor of ˜25 in a four month period. Our two new Chandra observations show a factor of ˜10 increase in the 0.35-8 keV X-ray luminosity, from ˜4 × 1036 to 4 × 1037 erg s-1 in ˜6 months, and the X-ray spectrum is consistent in both observations with a power-law with a photon index of Γ ˜ 0. We find evidence of X-ray spectral state changes: when SN 2010da is in a high-luminosity state, the X-ray spectrum is harder (Γ ˜0) compared to the low-luminosity state (Γ ˜ 1.2 ± 0.8). Using our Hubble observations, we fit the colour-magnitude diagram of the coeval stellar population to estimate a time since formation of the SN 2010da progenitor system of ≲5 Myr. Our observations are consistent with SN 2010da being a high-mass X-ray binary (HMXB) composed of a neutron star and a luminous blue variable-like companion, although we cannot rule out the possibility that SN 2010da is an unusually X-ray bright massive star. The ≲5 Myr age is consistent with the theoretically predicted delay time between the formation of a massive binary and the onset of the HMXB phase. It is possible that the initial 2010 outburst marked the beginning of X-ray production in the system, making SN 2010da possibly the first massive progenitor binary ever observed to evolve into an HMXB.

  12. Pharmacokinetics of chlorogenic acid and corydaline in DA-9701, a new botanical gastroprokinetic agent, in rats.

    PubMed

    Jung, Ji Won; Kim, Ju Myung; Jeong, Jin Seok; Son, Miwon; Lee, Hye Suk; Lee, Myung Gull; Kang, Hee Eun

    2014-07-01

    1.Few studies describing the pharmacokinetic properties of chlorogenic acid (CA) and corydaline (CRD) which are marker compounds of a new prokinetic botanical agent, DA-9701, have been reported. The aim of the present study is to evaluate the pharmacokinetic properties CA and CRD following intravenous and oral administration of pure CA (1-8 mg/kg) or CRD (1.1-4.5 mg/kg) and their equivalent dose of DA-9701 to rats. 2.  Dose-proportional AUC and dose-independent clearance (10.3-12.1 ml/min/kg) of CA were observed following its administration. Oral administration of CA as DA-9701 did not influence the oral pharmacokinetic parameters of CA. Incomplete absorption of CA, its decomposition in the gastrointestinal tract, and/or pre-systemic metabolism resulted in extremely low oral bioavailability (F) of CA (0.478-0.899%). 3.  CRD showed greater dose-normalized AUC in the higher dose group than that in lower dose group(s) after its administration due to saturation of its metabolism via decreased non-renal clearance (by 51.3%) and first-pass extraction. As a result, the F of CRD following 4.5 mg/kg oral CRD (21.1%) was considerably greater than those of the lower dose groups (9.10 and 13.8%). However, oral administration of CRD as DA-9701 showed linear pharmacokinetics as a result of increased AUC and F in lower-dose groups (by 182% and 78.5%, respectively) compared to those of pure CRD. The greater oral AUC of CRD for DA-9701 than for pure CRD could be due to decreased hepatic and/or GI first-pass extraction of CRD by other components in DA-9701.

  13. Integration of PanDA workload management system with Titan supercomputer at OLCF

    NASA Astrophysics Data System (ADS)

    De, K.; Klimentov, A.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.

    2015-12-01

    The PanDA (Production and Distributed Analysis) workload management system (WMS) was developed to meet the scale and complexity of LHC distributed computing for the ATLAS experiment. While PanDA currently distributes jobs to more than 100,000 cores at well over 100 Grid sites, the future LHC data taking runs will require more resources than Grid computing can possibly provide. To alleviate these challenges, ATLAS is engaged in an ambitious program to expand the current computing model to include additional resources such as the opportunistic use of supercomputers. We will describe a project aimed at integration of PanDA WMS with Titan supercomputer at Oak Ridge Leadership Computing Facility (OLCF). The current approach utilizes a modified PanDA pilot framework for job submission to Titan's batch queues and local data management, with light-weight MPI wrappers to run single threaded workloads in parallel on Titan's multicore worker nodes. It also gives PanDA new capability to collect, in real time, information about unused worker nodes on Titan, which allows precise definition of the size and duration of jobs submitted to Titan according to available free resources. This capability significantly reduces PanDA job wait time while improving Titan's utilization efficiency. This implementation was tested with a variety of Monte-Carlo workloads on Titan and is being tested on several other supercomputing platforms. Notice: This manuscript has been authored, by employees of Brookhaven Science Associates, LLC under Contract No. DE-AC02-98CH10886 with the U.S. Department of Energy. The publisher by accepting the manuscript for publication acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes.

  14. Identification of an abundant 56 kDa protein implicated in food allergy as granule-bound starch synthase.

    PubMed

    Krishnan, Hari B; Chen, Ming-Hsuan

    2013-06-01

    Rice, the staple food of south and east Asian counties, is considered to be hypoallergenic. However, several clinical studies have documented rice-induced allergy in sensitive patients. Rice proteins with molecular weights of 14-16, 26, 33, and 56 kDa have been identified as allergens. Recently, it was documented that the 56 kDa rice allergen was responsible for rice-induced anaphylaxis. The 14-16 kDa allergens have been identified as α-amylase inhibitors; the 26 kDa protein has been identified as α-globulin; and the 33 kDa protein has been identified as glyoxalase I. However, the identity of the 56 kDa rice allergen has not yet been determined. In this study, we demonstrate that serum from patients allergic to maize shows IgE binding to a 56 kDa protein that was present in both maize and rice but not in the oil seeds soybean and peanut. The 56 kDa IgE-binding protein was abundant in the rice endosperm. We have purified this protein from rice endosperm and demonstrated its reactivity to IgE antibodies from the serum of maize-allergic patients. The purified protein was subjected to matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry analysis, resulting in identification of this rice allergen as granule-bound starch synthase, a product of the Waxy gene. Immunoblot analysis using protein extracts from a waxy mutant of rice revealed the absence of the 56 kDa IgE-binding protein. Our results demonstrate that the 56 kDa rice allergen is granule-bound starch synthase and raise the possibility of using waxy mutants of rice as a potential source of the hypoallergenic diet for patients sensitized to the 56 kDa rice allergen.

  15. Infectious cDNA clones of the DA strain of Theiler's murine encephalomyelitis virus.

    PubMed Central

    Roos, R P; Stein, S; Ohara, Y; Fu, J L; Semler, B L

    1989-01-01

    The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis viruses cause a persistent demyelinating infection, whereas the GDVII strain and other GDVII subgroup strains cause an acute lethal polioencephalomyelitis. We generated an infectious DA cDNA clone inserted into a transcription vector. Virus derived from transfection of transcripts produced a demyelinating disease indistinguishable from that of wild-type virus. The infectious clone provides a critical reagent for the production of interstrain recombinant viruses to help identify genetic loci responsible for the biological activities of the strains. Images PMID:2555569

  16. Spectroscopy of the DA white dwarfs - Automatic atmospheric parameterization and mass distribution

    NASA Technical Reports Server (NTRS)

    Mcmahan, Robert K.

    1989-01-01

    A method for the automatic calculation of the atmospheric parameters (Teff and log g) of hydrogen-rich degenerate stars from low-resolution spectra is described, and then applied to the spectra of 53 DA white dwarfs. A value for the width of the DA mass distribution of sigma M/solar-M not greater than +0.10 is obtained using the proposed approach. The data indicate that the distribution is asymmetrically skewed to low masses; however, there is also evidence of a high-mass non-Gaussian tail.

  17. Evolution of robots throughout history from Hephaestus to Da Vinci Robot.

    PubMed

    Iavazzo, Christos; Gkegke, Xanthi-Ekaterini D; Iavazzo, Paraskevi-Evangelia; Gkegkes, Ioannis D

    2014-01-01

    Da Vinci robot is increasingly used for operations adding the advantages of robots to the favor of medicine. This is a historical article with the aim to present the evolution of robots in the medical area from the time of ancient myths to Renaissance and finally to the current revolutionary applications. We endeavored to collect several elegant narratives on the topic. The use of imagination could help the reader to find similarities. A trip from the Greek myths of Hephaestus through Aristotle and Leonardo Da Vinci to the robots of Karel Capek and Isaac Asimov and finally the invention of the medical robots is presented.

  18. Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil

    PubMed Central

    Song, Ok-Ryul; Lee, Seung-Jin; Lee, Yong-Seok; Lee, Sang-Cheol; Kim, Keun-Ki; Choi, Yong-Lark

    2008-01-01

    A mineral phosphate solubilizing bacterium, Burkholderia cepacia DA23 has been isolated from cultivated soils. Phosphate-solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. When 3% of glucose concentration was used for carbon source, the strain had a marked mineral phosphate-solubilizing activity. Mineral phosphate solubilization was directly related to the pH drop by the strain. Analysis of the culture medium by high pressure liquid chromatography identified gluconic acid as the main organic acid released by Burkholderia cepacia DA23. Gluconic acid production was apparently the result of the glucose dehydrogenase activity and glucose dehydrogenase was affected by phosphate regulation. PMID:24031195

  19. Evolution of robots throughout history from Hephaestus to Da Vinci Robot.

    PubMed

    Iavazzo, Christos; Gkegke, Xanthi-Ekaterini D; Iavazzo, Paraskevi-Evangelia; Gkegkes, Ioannis D

    2014-01-01

    Da Vinci robot is increasingly used for operations adding the advantages of robots to the favor of medicine. This is a historical article with the aim to present the evolution of robots in the medical area from the time of ancient myths to Renaissance and finally to the current revolutionary applications. We endeavored to collect several elegant narratives on the topic. The use of imagination could help the reader to find similarities. A trip from the Greek myths of Hephaestus through Aristotle and Leonardo Da Vinci to the robots of Karel Capek and Isaac Asimov and finally the invention of the medical robots is presented. PMID:25811686

  20. DAEdALUS: A New Search for CP Violation in the Neutrino Sector

    NASA Astrophysics Data System (ADS)

    Carr, Rachel; DAEdALUS Collaboration

    2011-04-01

    The DAEdALUS experiment offers a novel approach to measuring CP violation in the neutrino sector. The design uses multiple cyclotrons to produce intense beams of neutrinos from the decay-at-rest of pions and muons. Short-baseline muon- to electron-antineutrino oscillations are observed via inverse beta decay in an ultra-large water Cerenkov detector at DUSEL. The experiment's high-statistics, low-background output yields a CP violation sensitivity competitive with that of LBNE. Together, the complementary designs of DAEdALUS and LBNE provide the highest sensitivity of any presently proposed CP violation search.

  1. SU-A-210-04: Panel Discussion

    SciTech Connect

    Stanford, J.

    2015-06-15

    The purpose of this student annual meeting is to address topics that are becoming more relevant to medical physicists, but are not frequently addressed, especially for students and trainees just entering the field. The talk is divided into two parts: medical billing and regulations. Hsinshun Wu – Why should we learn radiation oncology billing? Many medical physicists do not like to be involved with medical billing or coding during their career. They believe billing is not their responsibility and sometimes they even refuse to participate in the billing process if given the chance. This presentation will talk about a physicist’s long career and share his own experience that knowing medical billing is not only important and necessary for every young medical physicist, but that good billing knowledge could provide a valuable contribution to his/her medical physics development. Learning Objectives: The audience will learn the basic definition of Current Procedural Terminology (CPT) codes performed in a Radiation Oncology Department. Understand the differences between hospital coding and physician-based or freestanding coding. Apply proper CPT coding for each Radiation Oncology procedure. Each procedure with its specific CPT code will be discussed in detail. The talk will focus on the process of care and use of actual workflow to understand each CPT code. Example coding of a typical Radiation Oncology procedure. Special procedure coding such as brachytherapy, proton therapy, radiosurgery, and SBRT. Maryann Abogunde – Medical physics opportunities at the Nuclear Regulatory Commission (NRC) The NRC’s responsibilities include the regulation of medical uses of byproduct (radioactive) materials and oversight of medical use end-users (licensees) through a combination of regulatory requirements, licensing, safety oversight including inspection and enforcement, operational experience evaluation, and regulatory support activities. This presentation will explore the career options for medical physicists in the NRC, how the NRC interacts with clinical medical physicists, and a physicist’s experience as a regulator. Learning Objectives: Explore non-clinical career pathways for medical physics students and trainees at the Nuclear Regulatory Commission. Overview of NRC medical applications and medical use regulations. Understand the skills needed for physicists as regulators. Abogunde is funded to attend the meeting by her employer, the NRC.

  2. SU-A-210-03: Panel Discussion

    SciTech Connect

    Rodrigues, A.

    2015-06-15

    The purpose of this student annual meeting is to address topics that are becoming more relevant to medical physicists, but are not frequently addressed, especially for students and trainees just entering the field. The talk is divided into two parts: medical billing and regulations. Hsinshun Wu – Why should we learn radiation oncology billing? Many medical physicists do not like to be involved with medical billing or coding during their career. They believe billing is not their responsibility and sometimes they even refuse to participate in the billing process if given the chance. This presentation will talk about a physicist’s long career and share his own experience that knowing medical billing is not only important and necessary for every young medical physicist, but that good billing knowledge could provide a valuable contribution to his/her medical physics development. Learning Objectives: The audience will learn the basic definition of Current Procedural Terminology (CPT) codes performed in a Radiation Oncology Department. Understand the differences between hospital coding and physician-based or freestanding coding. Apply proper CPT coding for each Radiation Oncology procedure. Each procedure with its specific CPT code will be discussed in detail. The talk will focus on the process of care and use of actual workflow to understand each CPT code. Example coding of a typical Radiation Oncology procedure. Special procedure coding such as brachytherapy, proton therapy, radiosurgery, and SBRT. Maryann Abogunde – Medical physics opportunities at the Nuclear Regulatory Commission (NRC) The NRC’s responsibilities include the regulation of medical uses of byproduct (radioactive) materials and oversight of medical use end-users (licensees) through a combination of regulatory requirements, licensing, safety oversight including inspection and enforcement, operational experience evaluation, and regulatory support activities. This presentation will explore the career options for medical physicists in the NRC, how the NRC interacts with clinical medical physicists, and a physicist’s experience as a regulator. Learning Objectives: Explore non-clinical career pathways for medical physics students and trainees at the Nuclear Regulatory Commission. Overview of NRC medical applications and medical use regulations. Understand the skills needed for physicists as regulators. Abogunde is funded to attend the meeting by her employer, the NRC.

  3. The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit.

    PubMed Central

    Kokubo, T; Gong, D W; Roeder, R G; Horikoshi, M; Nakatani, Y

    1993-01-01

    Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in transcriptional regulation by facilitating promoter responses to various activators. cDNAs encoding the 110-kDa subunit of Drosophila TFIID (p110) were isolated with a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein. The entire cDNA sequence contains an open reading frame encoding a 921-amino acid polypeptide with a calculated molecular mass of 99,337 Da. The recombinant protein expressed in Sf9 cells via a baculovirus vector interacts directly with the 230-kDa subunit of TFIID (p230). Together with the previous observation that the TATA box-binding subunit of TFIID (TFIID tau or TBP) interacts directly with only p230 among the TFIID subunits, this result suggests that p110 forms a complex with TFIID tau via p230. A binding study using various p230 mutants indicated that both p110 and TFIID tau interact with the N-terminal 352-amino acid portion of p230, suggesting a functional communication between p110 and TFIID tau via p230 interactions. Images Fig. 2 Fig. 3 Fig. 4 Fig. 5 PMID:8327460

  4. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  5. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  6. 40 CFR 60.45Da - Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Alternative standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). 60.45Da Section 60.45Da Protection of Environment... standards for combined nitrogen oxides (NOX) and carbon monoxide (CO). (a) The owner or operator of...

  7. Authentication of official Da-huang by sequencing and multiplex allele-specific PCR of a short maturase K gene.

    PubMed

    Xu, Guojie; Wang, Xueyong; Liu, Chunsheng; Li, Weidong; Wei, Shengli; Liu, Ying; Cheng, Xiaoli; Liu, Juan

    2013-02-01

    Rhubarb (official Da-huang) is an important medicinal herb in Asia. Many adulterants of official Da-huang have been discovered in Chinese markets in recent years, which has resulted in adverse effects in medicinal treatment. Here, novel molecular markers based on a short maturase K (matK) gene were developed for authenticating official Da-huang. This study showed that all the species from official Da-huang were clustered together in one clade in the polygenetic trees based on short matK. Two highly conserved single nucleotide polymorphisms of short matK were mined in the species from official Da-huang. Based on these polymophisms, four improved specific primers of official Da-huang were successfully developed that generated reproducible specific bands. These results suggest that the short matK sequence can be considered as a favorable candidate for distinguishing official Da-huang from its adulterants. The established multiplex allele-specific PCR was determined to be simple and accurate and may serve as a preferable tool for authentication of official Da-huang. In addition, we suggest that short-sized specific bands be developed to authenticate materials used in traditional Chinese medicine.

  8. Selective increase of in vivo firing frequencies in DA SN neurons after proteasome inhibition in the ventral midbrain.

    PubMed

    Subramaniam, Mahalakshmi; Kern, Beatrice; Vogel, Simone; Klose, Verena; Schneider, Gaby; Roeper, Jochen

    2014-09-01

    The impairment of protein degradation via the ubiquitin-proteasome system (UPS) is present in sporadic Parkinson's disease (PD), and might play a key role in selective degeneration of vulnerable dopamine (DA) neurons in the substantia nigra pars compacta (SN). Further evidence for a causal role of dysfunctional UPS in familial PD comes from mutations in parkin, which results in a loss of function of an E3-ubiquitin-ligase. In a mouse model, genetic inactivation of an essential component of the 26S proteasome lead to widespread neuronal degeneration including DA midbrain neurons and the formation of alpha-synuclein-positive inclusion bodies, another hallmark of PD. Studies using pharmacological UPS inhibition in vivo had more mixed results, varying from extensive degeneration to no loss of DA SN neurons. However, it is currently unknown whether UPS impairment will affect the neurophysiological functions of DA midbrain neurons. To answer this question, we infused a selective proteasome inhibitor into the ventral midbrain in vivo and recorded single DA midbrain neurons 2 weeks after the proteasome challenge. We found a selective increase in the mean in vivo firing frequencies of identified DA SN neurons in anesthetized mice, while those in the ventral tegmental area (VTA) were unaffected. Our results demonstrate that a single-hit UPS inhibition is sufficient to induce a stable and selective hyperexcitability phenotype in surviving DA SN neurons in vivo. This might imply that UPS dysfunction sensitizes DA SN neurons by enhancing 'stressful pacemaking'.

  9. 32 CFR 644.425 - Authority and procedure for disposal of surplus property by DA to eligible public agencies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true Authority and procedure for disposal of surplus property by DA to eligible public agencies. 644.425 Section 644.425 National Defense Department of Defense... property by DA to eligible public agencies. FPMR 101-47.303-2 provides that the disposal agency shall...

  10. The 37/67kDa laminin receptor (LR) inhibitor, NSC47924, affects 37/67kDa LR cell surface localization and interaction with the cellular prion protein

    PubMed Central

    Sarnataro, Daniela; Pepe, Anna; Altamura, Gennaro; De Simone, Imma; Pesapane, Ada; Nitsch, Lucio; Montuori, Nunzia; Lavecchia, Antonio; Zurzolo, Chiara

    2016-01-01

    The 37/67 kDa laminin receptor (LR) is a non-integrin protein, which binds both laminin-1 of the extracellular matrix and prion proteins, that hold a central role in prion diseases. The 37/67 kDa LR has been identified as interactor for the prion protein (PrPC) and to be required for pathological PrP (PrPSc) propagation in scrapie-infected neuronal cells, leading to the possibility that 37/67 kDa LR-PrPC interaction is related to the pathogenesis of prion diseases. A relationship between 37/67 kDa LR and PrPC in the presence of specific LR inhibitor compounds has not been investigated yet. We have characterized the trafficking of 37/67 kDa LR in both neuronal and non-neuronal cells, finding the receptor on the cell surface and nuclei, and identified the 67 kDa LR as the almost exclusive isoform interacting with PrPC. Here, we show that the treatment with the 37/67 kDa LR inhibitor, NSC47924, affects both the direct 37/67 kDa LR-PrPC interaction in vitro and the formation of the immunocomplex in live cells, inducing a progressive internalization of 37/67 kDa LR and stabilization of PrPC on the cell surface. These data reveal NSC47924 as a useful tool to regulate PrPC and 37/67 kDa LR trafficking and degradation, representing a novel small molecule to be tested against prion diseases. PMID:27071549

  11. Sobre a atividade pós-periélica do cometa de órbita parabólica Yanaka (1988r)

    NASA Astrophysics Data System (ADS)

    de Almeida, A. A.; Sanzovo, G. C.; Boczko, R.

    2003-08-01

    Greenberg, Singh & de Almeida (ApJ, 414: L45-48, 1993) mostraram que a deficiência nas abundâncias observadas de C2 e CN no Cometa Yanaka (1988r) pode ser explicada em termos das propriedades dos seus componentes refratários orgânicos, além do fato que trata-se de um cometa dinamicamente novo, observado através de abertura de fenda pequena projetada muito próximo do núcleo. Neste trabalho, complementamos o estudo sobre a atividade deste cometa de órbita parabólica, através da determinação da lei de potência que exprime sua taxa de produção de H2O (o principal indicador de atividade) na fase pós-periélica, determinamos o raio nuclear efetivo mínimo com sua fração de área ativa e analisamos a emissão de partículas de poeira observadas no contínuo em 625,0 nm.

  12. Effect of DA-9701 on the Normal Motility and Clonidine-induced Hypomotility of the Gastric Antrum in Rats

    PubMed Central

    Kang, Je Wook; Han, Dae Kyeong; Kim, Ock Nyun; Lee, Kwang Jae

    2016-01-01

    Background/Aims DA-9701 is a novel prokinetic agent. In the present study, we investigated the effect of DA-9701 on the motility of the gastric antrum in the normal and clonidine-induced hypomotility in an in vivo animal model. Methods A strain gauge force transducer was sutured on the gastric antrum to measure the contractile activity in rats. A total of 28 rats were subclassified into the 4 groups: (1) the placebo group, (2) the DA-9701 group, (3) the placebo group in the clonidine-pretreated rats, and (4) the DA-9701 group in the clonidine-pretreated rats. After the basal recording, either placebo (3% [w/v] hydroxypropylmethyl cellulose) or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. In the clonidine-pretreated rats, either placebo or DA-9701 was administered. Contractile signals were measured after the administration and after a meal. Results Oral administration of DA-9701 did not significantly alter the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the placebo group. The administration of clonidine decreased the motility index of the gastric antrum in the preprandial and postprandial periods, compared with the administration of placebo. This reduction of the antral motility by the administration of clonidine was not observed in the clonidine-pretreated DA-9701 group. The percentage of the motility index in the postprandial period was significantly greater in the clonidine-pretreated DA-9701 group, compared with the clonidine-pretreated placebo group. Conclusions DA-9701 improves the hypomotility of the gastric antrum induced by clonidine, suggesting its gastroprokinetic effect in the pathologic condition. PMID:26755679

  13. Effect of Fluosol-DA hemodilution on the kinetics of hepatically eliminated drugs.

    PubMed

    Shrewsbury, R P

    1987-03-01

    The pharmacokinetics of four hepatically eliminated drugs were determined in the rat following moderate hemodilution with either Fluosol-DA or normal saline. The drugs, antipyrine, phenytoin, indocyanine green, and d-propranolol, have extraction ratios from 0.01 to 0.99 in the rat. Animals received an intravenous dose of the drug 0.5, 24, 48, or 72 hours after hemodilution and drug disposition was compared to non-exchanged animals. Antipyrine clearance was increased 48 and 72 hours after Fluosol-DA exchange probably due to enhanced microsomal enzymatic activity. Indocyanine green clearance was decreased 24 hours after Fluosol-DA exchange more likely by a change in its extraction ratio than by alterations in hepatic blood flow. Phenytoin and d-propranolol clearances were decreased at 24 and 72 hours respectively after Fluosol-DA hemodilution due to a decreased apparent volume of distribution. Saline hemodilution decreased phenytoin clearance 24, 48, and 72 hours after exchange also by decreasing the apparent volume of distribution. This volume change was thought to be due to hypovolemia or a microcirculation redistribution which was secondary to hemodilution.

  14. MATERNAL ATRAZINE (ATR) ALTERS HYPOTHALAMIC DOPAMINE (HYP-DA) AND SERUM PROLACTIN (SPRL) IN MALE PUPS

    EPA Science Inventory

    Maternal Atrazine (ATR) alters hypothalamic dopamine (HYP-DA) and serum prolactin (sPRL) in male pups. 1Christopher Langdale, 2Tammy Stoker and 2Ralph Cooper. 1 Dept. of Cell Biology, North Carolina State University College of Veterinary Medicine, Raleigh, NC. 2 Endocrinology ...

  15. 40 CFR 60.44Da - Standards for nitrogen oxides (NOX).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false Standards for nitrogen oxides (NOX). 60... Steam Generating Units § 60.44Da Standards for nitrogen oxides (NOX). (a) Except as provided in... 50 percent or more of the total heat input to the combined cycle combustion turbine, the owner...

  16. 40 CFR 60.44Da - Standards for nitrogen oxides (NOX).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false Standards for nitrogen oxides (NOX). 60... Steam Generating Units § 60.44Da Standards for nitrogen oxides (NOX). (a) Except as provided in... 50 percent or more of the total heat input to the combined cycle combustion turbine, the owner...

  17. 40 CFR 60.44Da - Standards for nitrogen oxides (NOX).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false Standards for nitrogen oxides (NOX). 60... Steam Generating Units § 60.44Da Standards for nitrogen oxides (NOX). (a) Except as provided in... 50 percent or more of the total heat input to the combined cycle combustion turbine, the owner...

  18. A Integracao de Ensino das Ciencias da Saude (An Integrated Medical Education Program [in Brazil]).

    ERIC Educational Resources Information Center

    Pourchet-Campos, M. A.; Guimaraes Junior, Paulino

    At the Sixth Annual Reunion of the Brazilian Association of Medical Schools (VI Reuniao Anual da Associacao Brasileira de Escolas Medicas) leaders in the Brazilian medical profession proposed an integrated educational program for training students in the fields of medicine and public health. Under Brazil's present system of education, all…

  19. Long-term evolution of 1991 DA: A dynamically evolved extinct Halley-type comet

    NASA Technical Reports Server (NTRS)

    Hahn, Gerhard; Bailey, M. E.

    1992-01-01

    The long-term dynamical evolution of 21 variational orbits for the intermediate-period asteroid 1991 DA was followed for up to +/-10(exp 5) years from the present. 1991 DA is close to the 2:7 resonance with Jupiter; it has avoided close encounters, within 1 AU, with this planet for at least the past 30,000 years, even at the node crossing. The future evolution typically shows no close encounters with Jupiter within at least 50,000 years. This corresponds to the mean time between node crossings with either Jupiter or Saturn. Close encounters with Saturn and Jupiter lead to a chaotic evolution for the whole ensemble, while secular perturbations cause large-amplitude swings in eccentricity and inclination (the latter covering the range 15 deg approximately less than i approximately less than 85 deg) which correlate with deep excursions of the perihelion distance to values much less than 1 AU. These variations are similar to those found in P/Machholz and a variety of other high-inclination orbits, e.g., P/Hartley-IRAS. We emphasize the connection between the orbital evolution of 1991 DA and that of Halley-type comets. If 1991 DA was once a comet, it is not surprising that it is now extinct.

  20. 76 FR 55293 - Special Conditions: Diamond Aircraft Industries, Model DA-40NG; Electronic Engine Control (EEC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-07

    ... TRANSPORTATION Federal Aviation Administration 14 CFR Part 23 Special Conditions: Diamond Aircraft Industries... Diamond Aircraft Industries (DAI), model DA-40NG airplane. This airplane will have a novel or unusual... stamped and returned to the commenter. Background On May 11, 2010 Diamond Aircraft Industry GmbH...

  1. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 4 2012-07-01 2011-07-01 true DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  2. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 4 2013-07-01 2013-07-01 false DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  3. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 4 2010-07-01 2010-07-01 true DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  4. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 4 2011-07-01 2011-07-01 false DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  5. 32 CFR 644.512 - DA-SBA joint set-aside determination.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 4 2014-07-01 2013-07-01 true DA-SBA joint set-aside determination. 644.512 Section 644.512 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand...

  6. Views and Voices of the Working Class: Aída and Rosario

    ERIC Educational Resources Information Center

    Pao, Maria T.

    2014-01-01

    In 2005, Spanish television audiences saw the debut of the nation's first spinoff, the sitcom "Aída." The show featured the tribulations of its title character and her working-class family in their struggle to "llegar a fin de mes." It seemed to promise a sensibility enacted in the US series "Roseanne," where…

  7. 32 CFR 516.14 - Service of process on DA or Secretary of Army.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Service of process on DA or Secretary of Army. 516.14 Section 516.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF... or Secretary of Army. The Chief, Litigation Division, shall accept service of process for...

  8. 32 CFR 516.14 - Service of process on DA or Secretary of Army.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Service of process on DA or Secretary of Army. 516.14 Section 516.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF... or Secretary of Army. The Chief, Litigation Division, shall accept service of process for...

  9. Wearable augmented reality system using an IrDA device and a passometer

    NASA Astrophysics Data System (ADS)

    Tenmoku, Ryuhei; Kanbara, Masayuki; Yokoya, Naokazu

    2003-05-01

    This paper describes a wearable augmented reality system with an IrDA device and a passometer. To realize augmented reality systems, the position and orientation of user's viewpoint should be obtained in real time for aligning the real and virtual coordinate systems. In the proposed system, the orientation of user's viewpoint is measured by an inertial sensor attached to the user's glasses, and the position is measured by using an IrDA device and a passometer. First, the user's position is specified exactly when the user comes into the infrared ray range of the IrDA markers which are set up to the appointed points. When the user goes out of the infrared ray range, the user's position is estimated by using a passometer. The passometer is constructed of an electronic compass and acceleration sensors. The former can detect the user's walking direction. The latter can count how many steps the user walks. These data and the user's pace make it possible to estimate the user's position in the neighborhood of the IrDA markers. We have developed a navigation system based on using the techniques above and have proven the feasibility of the system with experiments.

  10. Leonardo da Vinci, One Year on...a Different Look at Vocational Training in Europe.

    ERIC Educational Resources Information Center

    Le Magazine, 1996

    1996-01-01

    Discusses the success of the Leonardo da Vinci program, a European laboratory of innovation in vocational training, a priority focus of investment in human resources and intelligence, and a way to mobilize innovative forces beyond national boundaries. Trends identified by the program focus on new information and communication technologies. (JOW)

  11. Transparency of Vocational Qualifications: The Leonardo da Vinci Approach. CEDEFOP Panorama Series.

    ERIC Educational Resources Information Center

    Bjornavold, Jens; Pettersson, Sten

    This report gives an overview of the situation of transparency of vocational qualifications by presenting measures introduced at the European Community level and by drawing attention to projects within the Leonardo da Vinci Program dealing with the issue. A 16-page executive summary appears first. Chapter 1 provides general background and aims.…

  12. Uniform remote control of front end processors in PAN-DA

    SciTech Connect

    Berg, D.; Berman, E.; MacKinnon, B.; Nicinski, T.; Oleynik, G.; Petravick, D.; Pordes, R.; Sergey, G.; Slimmer, D.; Streets, J.; White, V.

    1991-06-01

    The PAN-DA data acquisition software system encompasses support for a variety of front end processors including the Struck General Purpose Master (GPM), the Fermilab Smart Crate Controller (FSCC), and the Motorola MVME133-A VME single board computer. PAN-DA provides support for the remote control of these processors through its Remote Procedure eXecution (RPX) software. This software runs over serial lines, and in some PAN-DA environments, over the Ethernet and TCP/IP as well. Experiments E791 and E771 have successfully used RPX software in both FSCC software development and data collection during the 1990--1991 fixed target run at Fermilab. RPX software has facilitated development and testing of the Fermilab Silicon Strip Detector readout system, for which the FSCC is an integral component. RPX based control and monitoring of the GPM and the MVME133-A are essential parts of the PAN-DA data acquisition system used by E687 and E773 during the 1990--1991 run. 6 refs., 2 figs.

  13. Translocation of an 89-kDa periplasmic protein is associated with Holospora infection

    SciTech Connect

    Iwatani, Koichi; Dohra, Hideo; Lang, B. Franz; Burger, Gertraud; Hori, Manabu; Fujishima, Masahiro . E-mail: fujishim@yamaguchi-u.ac.jp

    2005-12-02

    The symbiotic bacterium Holospora obtusa infects the macronucleus of the ciliate Paramecium caudatum. After ingestion by its host, an infectious form of Holospora with an electron-translucent tip passes through the host digestive vacuole and penetrates the macronuclear envelope with this tip. To investigate the underlying molecular mechanism of this process, we raised a monoclonal antibody against the tip-specific 89-kDa protein, sequenced this partially, and identified the corresponding complete gene. The deduced protein sequence carries two actin-binding motifs. Indirect immunofluorescence microscopy shows that during escape from the host digestive vacuole, the 89-kDa proteins translocates from the inside to the outside of the tip. When the bacterium invades the macronucleus, the 89-kDa protein is left behind at the entry point of the nuclear envelope. Transmission electron microscopy shows the formation of fine fibrous structures that co-localize with the antibody-labeled regions of the bacterium. Our findings suggest that the 89-kDa protein plays a role in Holospora's escape from the host digestive vacuole, the migration through the host cytoplasm, and the invasion into the macronucleus.

  14. Brominated and radioiodinated derivatives of methylphenidate (MP): Potential imaging agents for the dopamine (DA) transporter

    SciTech Connect

    Pan, D.; Gatley, S.J.; Dewey, S.L.

    1994-05-01

    MP (Ritalin) is a psychomotor stimulant used in the treatment of attention-deficit hyperactivity disorder. The therapeutic properties of MP are thought to be mediated by its binding to a site on the DA transporter, resulting in inhibition of DA reuptake and enhanced levels of synaptic dopamine. MP also inhibits reuptake of norepinephrine (NE) in vitro. MP has two chiral centers, but its pharmacological activity is believed due solely to the d-threo isomer. We have found that d,l-threo-C-11 MP has favorable properties for PET studies, and therefore examined the effects of incorporating halogen atoms into the phenyl ring of MP, with a view to preparing C-11 and I-123 MP analogs as potential PET/SPECT tracers. We synthesized the 2-, 3- and 4-bromo MP analogs from the corresponding bromophenylacetonitriles by modification of the original synthesis of MP. In in vitro binding assays all three d,l-threo bromo compounds had higher affinities than MP for DA transporter sites labeled with tritiated WIN 35,428 (3->4-, 2->MP). They also showed high activity with NE reuptake sites labeled with tritiated nisoxetine. They were active in vivo as demonstrated by inhibition of heart uptake of tritiated NE in the mouse, and elevation of striatal extracellular DA (microdialysis) and stimulation of locomotor activity in the rat.

  15. Assessments of the economic impacts of natural hazards in Albania: application of DaLA methodology.

    NASA Astrophysics Data System (ADS)

    Massabo, Marco; Toto, Emanuela; Deda, Miranda; Prenci, Shemsi; Dhima, Maksimiljan

    2015-04-01

    Disaster loss datasets are built using economic assessments done by different parties and using different approaches which are in many cases inconsistent. This is observed in more than 75% of the Alabanian records with missing economic evaluations. To assess the economic impact of disasters at all scales in Global Assessment Report on Disaster Risk Reduction 2011 and 2013 has used the DaLA methodology that takes advantage of a common and homogeneous set of quantitative physical damage indicators contained in the disaster databases. These have to be taken as the lower bound of the damage, which in general is higher. The UN-ECLAC DaLA Methodology takes into account in its assessments the overall status of economy of the affected country. It uses the national accounts and statistics of the country government as baseline data to assess damage and loss. It also factors in the impact of disasters on individual livelihoods and incomes to fully define the needs for recovery and reconstruction. Similarly to national disaster loss databases DaLA assessments have a national level of observation and data produced at sub-national resolution, resulting in highly detailed and accurate data sets. In 2013 in Albania was implemented a collection of disaster losses based on Desinventar. We applied the DaLa methodology to the Albanian database of impacts of natural disasters.

  16. Computerized Dynamic Assessment (C-DA): Diagnosing L2 Development According to Learner Responsiveness to Mediation

    ERIC Educational Resources Information Center

    Poehner, Matthew E.; Zhang, Jie; Lu, Xiaofei

    2015-01-01

    Dynamic assessment (DA) derives from the sociocultural theory of mind as elaborated by Russian psychologist L. S. Vygotsky. By offering mediation when individuals experience difficulties and carefully tracing their responsiveness, Vygotsky (1998) proposed that diagnoses may uncover abilities that have fully formed as well as those still in the…

  17. Understanding and Interpreting Japanese NP1 "wa" NP2 "da" Sentences: Mechanism and Contextual Factors

    ERIC Educational Resources Information Center

    Yoshida, Megumi

    2013-01-01

    This dissertation investigates the contextual factors that affect the understanding and interpretation of one Japanese topicalized construction, NP[subscript 1] wa NP[subscript 2] da sentences, by native speakers of Japanese. The construction allows two possibilities in the relation between the NP[subscript 1] and the NP[subscript 2]. When the two…

  18. Validation of the Delvotest SP NT DA. Performance Tested Method 011101.

    PubMed

    Hennart, Stephen L A; Faragher, John

    2012-01-01

    Delvotest SP NT DA is designed to test milk for the presence of antibacterial substances, such as antibiotics. The test is made of an agar gel containing bacterial spores and a color indicator. The milk sample is added onto the agar gel, and the test is incubated at 64 degrees C. The principle of the test is based on the diffusion of possible inhibitory substances that may be present in the milk sample into agar. This reduces growth and acid production by the test organism, and delays or prevents the agar from changing color from purple to yellow. The Delvotest Accelerator is an automated system in which the plates containing the milk to be analyzed are placed for incubation. The Accelerator automatically detects the end of the incubation and reads the results. A sample containing antibiotic will be noted as "positive." A sample without antibiotics or with antibiotics at concentrations below detection level will be noted as "negative." The present report includes all technical details about the Delvotest SP NT DA, and the results of the validation study. The validation study demonstrates that the Delvotest SP NT DA conforms to the product performance claims and confirms the robustness of the test. The Delvotest SP NT DA is, therefore, granted Performance Tested Method certification.

  19. Validation of the Delvotest SP NT DA. Performance Tested Method 011101.

    PubMed

    Hennart, Stephen L A; Faragher, John

    2012-01-01

    Delvotest SP NT DA is designed to test milk for the presence of antibacterial substances, such as antibiotics. The test is made of an agar gel containing bacterial spores and a color indicator. The milk sample is added onto the agar gel, and the test is incubated at 64 degrees C. The principle of the test is based on the diffusion of possible inhibitory substances that may be present in the milk sample into agar. This reduces growth and acid production by the test organism, and delays or prevents the agar from changing color from purple to yellow. The Delvotest Accelerator is an automated system in which the plates containing the milk to be analyzed are placed for incubation. The Accelerator automatically detects the end of the incubation and reads the results. A sample containing antibiotic will be noted as "positive." A sample without antibiotics or with antibiotics at concentrations below detection level will be noted as "negative." The present report includes all technical details about the Delvotest SP NT DA, and the results of the validation study. The validation study demonstrates that the Delvotest SP NT DA conforms to the product performance claims and confirms the robustness of the test. The Delvotest SP NT DA is, therefore, granted Performance Tested Method certification. PMID:22468368

  20. Specific binding of victorin to a 100-kDa protein from oats

    SciTech Connect

    Wolpert, T.J.; Macko, V. )

    1989-06-01

    Susceptibility of oats to victoria blight, caused by the fungus Cochliobolus victoriae, and sensitivity to the host-specific toxin victorin, produced by the fungus, are controlled by the dominant allele at the Vb locus. It has been postulated that the Vb locus encodes a toxin receptor, although direct evidence for such a receptor is not available. Recent studies on structure-activity relationships of the toxin established a methodology for producing {sup 125}I-labeled victorin. Electrophoretic analysis of proteins from isogenic susceptible and resistant oat genotypes following treatment of leaves with radiolabeled victorin showed that victorin binds in a covalent and a genotype-specific manner to a 100-kDa protein only in susceptible oat leaf slices. This in vivo binding was competitively displaced by reduced victorin, a nontoxic protective compound, and appeared to be correlated with biological activity. In vitro binding to the 100-kDa protein in leaf extracts showed several differences from in vivo binding. Binding was not genotype specific and required a reducing agent that was not required for in vivo binding. Differential centrifugation showed that the 100-kDa victorin binding protein was not a cytosolic protein but was enriched in a high-speed particulate fraction. The data support the hypothesis that the 100-kDa protein is the victorin receptor.

  1. 32 CFR 516.14 - Service of process on DA or Secretary of Army.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 3 2012-07-01 2009-07-01 true Service of process on DA or Secretary of Army. 516.14 Section 516.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF... or Secretary of Army. The Chief, Litigation Division, shall accept service of process for...

  2. 32 CFR 516.14 - Service of process on DA or Secretary of Army.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 3 2013-07-01 2013-07-01 false Service of process on DA or Secretary of Army. 516.14 Section 516.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF... or Secretary of Army. The Chief, Litigation Division, shall accept service of process for...

  3. 32 CFR 516.14 - Service of process on DA or Secretary of Army.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 3 2014-07-01 2014-07-01 false Service of process on DA or Secretary of Army. 516.14 Section 516.14 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY AID OF... or Secretary of Army. The Chief, Litigation Division, shall accept service of process for...

  4. Modifications of transaxillary approach in endoscopic da Vinci-assisted thyroid and parathyroid gland surgery.

    PubMed

    Al Kadah, Basel; Piccoli, Micaela; Mullineris, Barbara; Colli, Giovanni; Janssen, Martin; Siemer, Stephan; Schick, Bernhard

    2015-03-01

    Endoscopic surgery for treatment of thyroid and parathyroid pathologies is increasingly gaining attention. The da Vinci system has already been widely used in different fields of medicine and quite recently in thyroid and parathyroid surgery. Herein, we report about modifications of the transaxillary approach in endoscopic surgery of thyroid and parathyroid gland pathologies using the da Vinci system. 16 patients suffering from struma nodosa in 14 cases and parathyroid adenomas in two cases were treated using the da Vinci system at the ENT Department of Homburg/Saar University and in cooperation with the Department of General Surgery in New Sant'Agostino Hospital, Modena/Italy. Two different retractors, endoscopic preparation of the access and three different incision modalities were used. The endoscopic preparation of the access allowed us to have a better view during preparation and reduced surgical time compared to the use of a headlamp. To introduce the da Vinci instruments at the end of the access preparation, the skin incisions were over the axilla with one incision in eight patients, two incisions in four patients and three incisions in a further four patients. The two and three skin incisions modality allowed introduction of the da Vinci instruments without arm conflicts. The use of a new retractor (Modena retractor) compared to a self-developed retractor made it easier during the endoscopic preparation of the access and the reposition of the retractor. The scar was hidden in the axilla and independent of the incisions selected, the cosmetic findings were judged by the patients to be excellent. The neurovascular structures such as inferior laryngeal nerve, superior laryngeal nerve and vessels, as well as the different pathologies, were clearly 3D visualized in all 16 cases. No paralysis of the vocal cord was observed. All patients had a benign pathology in their histological examination. The endoscopic surgery of the thyroid and parathyroid gland can be

  5. Observation campaign for the near-Earth object 2012 DA_{14} in Japan

    NASA Astrophysics Data System (ADS)

    Urakawa, S.; Terai, T.; Takahashi, J.; Fujii, M.; Hanayama, H.; Yoshida, F.; Hoshi, H.; Sato, T.; Ushioda, K.; Oasa, Y.; Arai, A.; Honda, S.; Takagi, Y.; Itoh, Y.; Ishiguro, M.

    2014-07-01

    We present the observation campaign for the near-Earth object 2012 DA_{14} (hereafter, DA_{14}). The asteroid DA_{14} came close to the Earth's surface on 2013 February 15, 19:33 UT, at a distance of 27,700 km. DA_{14}'s altitude from the horizon in Japan was ˜ 30° at the closest approach time. The estimated diameter of DA_{14} is ˜50 m. The close encounter and the horizontal altitude made it possible to conduct a variety of observations for this tiny asteroid using small- and medium-sized telescopes. There have been few detailed observations, including spectroscopy, of asteroids smaller than 100 m in diameter. Moreover, the close encounter might results in a change for the rotational period and the surface spectrum due to the tidal forces. The observations around the closest approach are useful to investigate such physical changes. We performed visible spectroscopy, near-infrared photometry, and time-series photometry in the visible wavelength region. The visible spectroscopy was conducted using the 0.4-m F/10 telescope at the Fujii Kurosaki Observatory. The observations aimed to deduce the taxonomy of DA_{14}. The near-infrared photometry was made by Nishiharima Infrared Camera (NIC) attached to the 2.0-m Nayuta telescope at the Nishi-Harima Astronomical Observatory. The NIC achieves three-band (i.e., J, H, and K_{s}) simultaneous observations, which allowed us to reliably deduce the colors of this fast-moving object. The time-series photometry was carried out using the 0.55-m telescope at Saitama University. The solar phase angle of DA_{14} had varied widely around its closest approach but was almost constant during the following night. The R-band images were obtained continuously over 2 h at the closest approach and for about 5 h on the subsequent night. Those two nights of observations allow us to determine the rotational properties and the phase effect. We found, from the visible spectroscopy [1], that DA_{14} is an L-type asteroid. The flatness of the

  6. PanDA Pilot Submission using Condor-G: Experience and Improvements

    NASA Astrophysics Data System (ADS)

    Zhao, Xin; Hover, John; Wlodek, Tomasz; Wenaus, Torre; Frey, Jaime; Tannenbaum, Todd; Livny, Miron; ATLAS Collaboration

    2011-12-01

    PanDA (Production and Distributed Analysis) is the workload management system of the ATLAS experiment, used to run managed production and user analysis jobs on the grid. As a late-binding, pilot-based system, the maintenance of a smooth and steady stream of pilot jobs to all grid sites is critical for PanDA operation. The ATLAS Computing Facility (ACF) at BNL, as the ATLAS Tier1 center in the US, operates the pilot submission systems for the US. This is done using the PanDA "AutoPilot" scheduler component which submits pilot jobs via Condor-G, a grid job scheduling system developed at the University of Wisconsin-Madison. In this paper, we discuss the operation and performance of the Condor-G pilot submission at BNL, with emphasis on the challenges and issues encountered in the real grid production environment. With the close collaboration of Condor and PanDA teams, the scalability and stability of the overall system has been greatly improved over the last year. We review improvements made to Condor-G resulting from this collaboration, including isolation of site-based issues by running a separate Gridmanager for each remote site, introduction of the 'Nonessential' job attribute to allow Condor to optimize its behavior for the specific character of pilot jobs, better understanding and handling of the Gridmonitor process, as well as better scheduling in the PanDA pilot scheduler component. We will also cover the monitoring of the health of the system.

  7. PanDA Pilot Submission using Condor-G: Experience and Improvements

    SciTech Connect

    Zhao X.; Hover John; Wlodek Tomasz; Wenaus Torre; Frey Jaime; Tannenbaum Todd; Livny Miron

    2011-01-01

    PanDA (Production and Distributed Analysis) is the workload management system of the ATLAS experiment, used to run managed production and user analysis jobs on the grid. As a late-binding, pilot-based system, the maintenance of a smooth and steady stream of pilot jobs to all grid sites is critical for PanDA operation. The ATLAS Computing Facility (ACF) at BNL, as the ATLAS Tier1 center in the US, operates the pilot submission systems for the US. This is done using the PanDA 'AutoPilot' scheduler component which submits pilot jobs via Condor-G, a grid job scheduling system developed at the University of Wisconsin-Madison. In this paper, we discuss the operation and performance of the Condor-G pilot submission at BNL, with emphasis on the challenges and issues encountered in the real grid production environment. With the close collaboration of Condor and PanDA teams, the scalability and stability of the overall system has been greatly improved over the last year. We review improvements made to Condor-G resulting from this collaboration, including isolation of site-based issues by running a separate Gridmanager for each remote site, introduction of the 'Nonessential' job attribute to allow Condor to optimize its behavior for the specific character of pilot jobs, better understanding and handling of the Gridmonitor process, as well as better scheduling in the PanDA pilot scheduler component. We will also cover the monitoring of the health of the system.

  8. Site effects inferred from HVSR methodology in Praia da Vitória city (Portugal)

    NASA Astrophysics Data System (ADS)

    Fontiela, João; Borges, José; Bezzeghoud, Mourad; Rosset, Philippe; Rodrigues, Francisco

    2016-04-01

    Terceira island (Azores) have experienced several strong earthquakes, especially on the eastern and south-eastern part of the island with maximum MM intensity of VIII in 1800, 1801 and 1912 and IX in 1614 and 1841. Seismicity of the eastern part of the island is largely dominated by the tectonic of the NW-SE Lajes graben where are located Praia da Vitória and other setlements. Praia da Vitória is settled partially over pyroclastic material(pumice and ignimbrite) deposited around 20'000 years ago and forming part of the UpperTerceira Group. Its thickness is 15m on the north but in Praia da Vitoria has less than one meter.. It is sited on by basaltic flow of the upper Basaltic Terceira Group (2,000y BP) and a thick andesitic lava flow. A narrow band with sand dunes and beach crossed this deposits along Praia da Vitória and behind it appear a flat area with 7-10m of round cobbles that is interpreted as an old boulders'beach. Thickness of these different deposits are tricky to estimate in urban areas since very few outcrops are present. In order to assess site amplification of seismic waves due to the soil conditions, we perform horizontal-to-vertical spectral ratio (HVSR) method from broad band ambient noise measurements in 51 different sites within Praia da Vitoria. The first results show that the frequencies of the study area are variable, lying between 0.6-12 Hz. ACKNOWLEDGMENTS: João Fontiela is supported by grant M3.1.2/F/060/2011 provided by the Regional Science Fund of the Azores Regional Government. This study was partially funded by the Institute of Earth Sciences, Portugal, under the contract with the FCT, ICT-UID/GEO/04683/2013 (Portugal).

  9. Local classification: Locally weighted-partial least squares-discriminant analysis (LW-PLS-DA).

    PubMed

    Bevilacqua, Marta; Marini, Federico

    2014-08-01

    The possibility of devising a simple, flexible and accurate non-linear classification method, by extending the locally weighted partial least squares (LW-PLS) approach to the cases where the algorithm is used in a discriminant way (partial least squares discriminant analysis, PLS-DA), is presented. In particular, to assess which category an unknown sample belongs to, the proposed algorithm operates by identifying which training objects are most similar to the one to be predicted and building a PLS-DA model using these calibration samples only. Moreover, the influence of the selected training samples on the local model can be further modulated by adopting a not uniform distance-based weighting scheme which allows the farthest calibration objects to have less impact than the closest ones. The performances of the proposed locally weighted-partial least squares-discriminant analysis (LW-PLS-DA) algorithm have been tested on three simulated data sets characterized by a varying degree of non-linearity: in all cases, a classification accuracy higher than 99% on external validation samples was achieved. Moreover, when also applied to a real data set (classification of rice varieties), characterized by a high extent of non-linearity, the proposed method provided an average correct classification rate of about 93% on the test set. By the preliminary results, showed in this paper, the performances of the proposed LW-PLS-DA approach have proved to be comparable and in some cases better than those obtained by other non-linear methods (k nearest neighbors, kernel-PLS-DA and, in the case of rice, counterpropagation neural networks).

  10. [Prevalence of Pre-Obesity/Obesity in Pre and Basic School Children at Vila Nova de Gaia, Portugal].

    PubMed

    Camarinha, Bárbara; Graça, Pedro; Nogueira, Paulo Jorge

    2016-01-01

    Introdução: Mais de 30% das crianças portuguesas apresentam excesso de peso. Sendo um grave problema de saúde pública com múltiplas causas, só uma ação intersectorial e concertada poderá contribuir para a sua resolução. As autarquias possuem um conjunto de competências que as tornam ideais para uma intervenção eficaz na luta contra este problema. Para essa ação ser efetiva é necessária uma avaliação local da dimensão do problema e sua distribuição espacial. O objetivo deste estudo foi caracterizar, do ponto de vista da prevalência de excesso de peso, a população do pré-escolar e primeiro ciclo do ensino básico sob responsabilidade da autarquia de Vila Nova de Gaia, o terceiro município mais populoso do país. Material e Métodos: Avaliaram-se 8 974 crianças do pré-escolar e primeiro ciclo do ensino básico do município, no ano letivo 2013/14. A avaliação do estado nutricional da amostra foi feita com recurso às medidas antropométricas peso e altura. Na classificação do estado nutricional considerou-se os percentis ou z-scores do índice de massa corporal para a idade de acordo com os critérios definidos pela Organização Mundial de Saúde, Centers for Disease Control and Prevention e pela International Obesity Task Force. Resultados: A prevalência de excesso de peso pelo critério da Organização Mundial da Saúde foi de 37,4%. Identificaram-se disparidades na distribuição do excesso de peso nas escolas do concelho. Discussão: Os resultados encontrados foram ligeiramente superiores aos de outros estudos nacionais e internacionais, podendo a abrangência amostral justificar as diferenças encontradas. Conclusão: A prevalência encontrada é elevada e de distribuição aparentemente distinta a nível espacial, o que poderá contribuir para a identificação dos principais determinantes do problema a nível local.

  11. The Effect of DA-6034 on Intestinal Permeability in an Indomethacin-Induced Small Intestinal Injury Model

    PubMed Central

    Kwak, Dong Shin; Lee, Oh Young; Lee, Kang Nyeong; Jun, Dae Won; Lee, Hang Lak; Yoon, Byung Chul; Choi, Ho Soon

    2016-01-01

    Background/Aims DA-6034 has anti-inflammatory activities and exhibits cytoprotective effects in acute gastric injury models. However, explanations for the protective effects of DA-6034 on intestinal permeability are limited. This study sought to investigate the effect of DA-6034 on intestinal permeability in an indomethacin-induced small intestinal injury model and its protective effect against small intestinal injury. Methods Rats in the treatment group received DA-6034 from days 0 to 2 and indomethacin from days 1 to 2. Rats in the control group received indomethacin from days 1 to 2. On the fourth day, the small intestines were examined to compare the severity of inflammation. Intestinal permeability was evaluated by using fluorescein isothiocyanate-labeled dextran. Western blotting was performed to confirm the association between DA-6034 and the extracellular signal-regulated kinase (ERK) pathway. Results The inflammation scores in the treatment group were lower than those in the control group, but the difference was statistically insignificant. Hemorrhagic lesions in the treatment group were broader than those in the control group, but the difference was statistically insignificant. Intestinal permeability was lower in the treatment group than in the control group. DA-6034 enhanced extracellular signal-regulated kinase expression, and intestinal permeability was negatively correlated with ERK expression. Conclusions DA-6034 may decrease intestinal permeability in an indomethacin-induced intestinal injury model via the ERK pathway. PMID:27114435

  12. Dioscorea Extract (DA-9801) Modulates Markers of Peripheral Neuropathy in Type 2 Diabetic db/db Mice.

    PubMed

    Moon, Eunjung; Lee, Sung Ok; Kang, Tong Ho; Kim, Hye Ju; Choi, Sang Zin; Son, Mi-Won; Kim, Sun Yeou

    2014-09-01

    The purpose of this study was to investigate the therapeutic effects of DA-9801, an optimized extract of Dioscorea species, on diabetic peripheral neuropathy in a type 2 diabetic animal model. In this study, db/db mice were treated with DA-9801 (30 and 100 mg/kg, daily, p.o.) for 12 weeks. DA-9801 reduced the blood glucose levels and increased the withdrawal latencies in hot plate tests. Moreover, it prevented nerve damage based on increased nerve conduction velocity and ultrastructural changes. Decrease of nerve growth factor (NGF) may have a detrimental effect on diabetic neuropathy. We previously reported NGF regulatory properties of the Dioscorea genus. In this study, DA-9801 induced NGF production in rat primary astrocytes. In addition, it increased NGF levels in the sciatic nerve and the plasma of type 2 diabetic animals. DA-9801 also increased neurite outgrowth and mRNA expression of Tieg1/Klf10, an NGF target gene, in PC12 cells. These results demonstrated the attenuation of diabetic peripheral neuropathy by oral treatment with DA-9801 via NGF regulation. DA-9801 is currently being evaluated in a phase II clinical study.

  13. The alpha- and beta-subunits of the jacalins are cleavage products from a 17-kDa precursor.

    PubMed

    Ngoc, L D; Brillard, M; Hoebeke, J

    1993-02-13

    The jacalins of three Artocarpus species were purified by affinity chromatography on a desialylated mucin-CNBr-Sepharose 4B column. The beta-chains and the 14 kDa alpha-chains were separated by high pressure liquid chromatography and the 17 kDa chains by preparative electrophoresis. The 17 kDa and 14 kDa chains had a similar highly conserved N-terminal sequence. The beta-chains were different for the three species and Artocarpus champeden contained two different beta-chains. CNBr cleavage of the 17 kDa polypeptide of Artocarpus tonkinensis yielded one peptide more than the 14 kDa. The N-terminal sequence of this fragment was similar to that of the beta-chain proving that this chain results from a proteolytic cleavage at the C-terminus of the 17 kDa peptide. The large heterogeneity of the beta-chains of jacalins from different species could be used as a marker for evolutionary studies on the Artocarpus family.

  14. [Characterization and Evolution of Avoidable Admissions in Portugal: The Impact of Two Methodologic Approaches].

    PubMed

    Sarmento, João; Alves, Conceição; Oliveira, Paula; Sebastião, Rita; Santana, Rui

    2015-01-01

    Introdução: O objectivo deste estudo é avaliar a performance dos sistemas de saúde através dos internamentos evitáveis, dado que estes têm vindo a ganhar importância a nível internacional. Foram utilizadas duas metodologias distintas para a identificação dos internamentos por Ambulatory Care Sensitive Conditions, fazendo uma descrição da realidade e a evolução destes em Portugal. Material e Métodos: Analisámos mais de 12 milhões de internamentos entre 2000 e 2012, utilizando as bases de dados de resumos de alta nacionais. Utilizaram-se duas metodologias de identificação das Ambulatory Care Sensitive Conditions, determinando a sua concordância. Calculámos cenários de melhoria potencial. Resultados: Em 2012, 4,4% e 32,5% dos internamentos por causas médicas seriam evitáveis segundo a metodologia canadiana e espanhola respectivamente. Os internamentos são mais frequentes nas crianças e nos idosos, divergindo as suas causas de acordo com o grupo etário e a metodologia utilizada. A taxa de internamentos no período em análise diminuiu 20% segundo a metodologia canadiana e aumentou 16% segundo a espanhola. Existem agregados regionais de performance acima e abaixo da média nacional. A concord ncia entre as duas metodologias é baixa. Estimaram-se reduções potenciais entre 20,3% e 53,5% dos internamentos.Discussão: Os internamentos evitáveis assumem um volume considerável em Portugal. Apesar de em teoria serem evitáveis a sua eliminação por completo não é uma possibilidade prática, no entanto, o seu estudo possibilita a avaliação e monitorização de resultados e o estabelecimento de prioridades de intervenção. Conclusão: Para uma caracterização mais precisa dos internamentos evitáveis é necessário consensualizar em Portugal a metodologia para a sua identificação.

  15. OV-Wav: um novo pacote para análise multiescalar em astronomia

    NASA Astrophysics Data System (ADS)

    Pereira, D. N. E.; Rabaça, C. R.

    2003-08-01

    Wavelets e outras formas de análise multiescalar têm sido amplamente empregadas em diversas áreas do conhecimento, sendo reconhecidamente superiores a técnicas mais tradicionais, como as análises de Fourier e de Gabor, em certas aplicações. Embora a teoria dos wavelets tenha começado a ser elaborada há quase trinta anos, seu impacto no estudo de imagens astronômicas tem sido pequeno até bem recentemente. Apresentamos um conjunto de programas desenvolvidos ao longo dos últimos três anos no Observatório do Valongo/UFRJ que possibilitam aplicar essa poderosa ferramenta a problemas comuns em astronomia, como a remoção de ruído, a detecção hierárquica de fontes e a modelagem de objetos com perfis de brilho arbitrários em condições não ideais. Este pacote, desenvolvido para execução em plataforma IDL, teve sua primeira versão concluída recentemente e está sendo disponibilizado à comunidade científica de forma aberta. Mostramos também resultados de testes controlados ao quais submetemos os programas, com a sua aplicação a imagens artificiais, com resultados satisfatórios. Algumas aplicações astrofísicas foram estudadas com o uso do pacote, em caráter experimental, incluindo a análise da componente de luz difusa em grupos compactos de galáxias de Hickson e o estudo de subestruturas de nebulosas planetárias no espaço multiescalar.

  16. [Identification and preservation of parathyroid glands in cadaver parts].

    PubMed

    Melo, Catarina; Bernardes, António; Carvalho, Lina

    2013-01-01

    Introdução: É indispensável ter um conhecimento profundo da morfologia da glândula tiróide e das estruturas com ela relacionadas no compartimento anterior do pescoço, para minimizar a morbilidade decorrente da cirurgia da tiróide, nomeadamente a lesão das glândulas paratiróides e dos nervos laríngeos. Este estudo pretendeu identificar glândulas paratiróides em peças de cadáver, confirmando-as histologicamente.Material e Métodos: Foram usadas 20 peças de cadáver para simular tiroidectomias. Durante a dissecção, foram isoladas as glândulas tiróides e eventuais glândulas paratiróides, que foram submetidas a estudo histológico.Discussão: Foram dissecadas 20 peças de cadáver (regiões cervicais anteriores), sendo isolados 48 fragmentos que correspondiam a eventuais glândulas paratiróides, dos quais 35 foram confirmados histologicamente como sendo efectivamente paratiróides. Os 20 casos foram, então, divididos em três grupos, de acordo com o número de paratiróides confirmadas histologicamente. No primeiro grupo, constituído por 11 casos, todas as eventuais paratiróides foram confirmadas. No segundo grupo, constituído por seis casos, apenas algumas paratiróides foram confirmadas. No terceiro grupo, constituído por três casos, nenhuma das eventuais paratiróides isoladas era efectivamente paratiróide. Em sete das 20 glândulas tiróides isoladas, foram identificadas oito paratiróides no estudo histológico: quatro sub-capsulares; três extra-capsulares e uma intra-tiróideia. As dimensões das paratiróides não tinham relação estatisticamente significativa.Conclusão: O conhecimento da anatomia das estruturas do compartimento central do pescoço e das suas variações mais frequentes diminui, mas não elimina a morbilidade da cirurgia da tiróide, nomeadamente a excisão iatrogénica das paratiróides, cuja dificuldade de identificação foi evidenciada nas peças dissecadas.

  17. Astronomy, Art and Mythology in a Public Elementary School in Itaocara/rj. (Spanish Title: Astronomía, Arte y Mitologia en el Ensino Fundamental en Una Escuela de la Red Estatal en ITAOCARA/RJ.) Astronomia, Arte e Mitologia no Ensino Fundamental em Escola da Rede Estadual em ITAOCARA/RJ

    NASA Astrophysics Data System (ADS)

    Oliveira Bernardes, Adriana; Ramos dos Santos, Arleidimar

    2008-12-01

    conocimientos recibidos. El trabajo en sí ha mostrado además de la posibilidad de la inclusión de la Astronomía en la Educación Primaria, la posibilidad de un trabajo interdisciplinario en los niveles iniciales incluyendo Astronomía, Arte y Mitología. Los resultados presentados por los estudiantes en las Olimpiadas promovidas en la escuela y por la OBA (Olimpiada Brasilera de Astronomía) permitieron verificar un creciente aprendizaje y estimulo hacia las materias científicas, comprobados por la apropiada expresión de los conceptos adquiridos de astronomía y presentados en evaluaciones o en informes obtenidos de ellos mismos, sus familias y profesores. Desenvolvendo um trabalho voluntário junto aos alunos do ensino fundamental no 1º ciclo (1ª a 4ª série), os monitores de Astronomia membros do CAIMP (Clube de Astronomia de Itaocara Marcos Pontes), que eram em sua maioria alunos do ensino médio, desenvolveram um trabalho de aproximação entre os alunos do Colégio Estadual Teotônio Brandão Vilela e os temas envolvendo Ciências e Astronomia. Através de oficinas de informática, artes, vídeos educativos e teatros de fantoches, os alunos puderam expressar seus conhecimentos e emoções diante das lendas mitológicas com as quais começaram a ter contato. O trabalho desenvolvido pelos monitores junto aos alunos proporcionou a integração entre as turmas do colégio e os levou a participação em atividades de maneira a estimular sua oralidade e aumentar sua auto-estima. Trabalhando várias formas de expressões, os alunos puderam realizar seus experimentos e conhecer alguns conceitos de Física e Astronomia, enquanto adquiriam autonomia para se expressarem de acordo com seus sentimentos e conhecimentos adquiridos. O trabalho em si mostrou além da possibilidade de inserção de Astronomia no Ensino Fundamental, a possibilidade de realização de um trabalho interdisciplinar nas séries iniciais envolvendo Astronomia, Arte e Mitologia. Os resultados apresentados

  18. Approaches to Sample Size Determination for Multivariate Data: Applications to PCA and PLS-DA of Omics Data.

    PubMed

    Saccenti, Edoardo; Timmerman, Marieke E

    2016-08-01

    Sample size determination is a fundamental step in the design of experiments. Methods for sample size determination are abundant for univariate analysis methods, but scarce in the multivariate case. Omics data are multivariate in nature and are commonly investigated using multivariate statistical methods, such as principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA). No simple approaches to sample size determination exist for PCA and PLS-DA. In this paper we will introduce important concepts and offer strategies for (minimally) required sample size estimation when planning experiments to be analyzed using PCA and/or PLS-DA. PMID:27322847

  19. Approaches to Sample Size Determination for Multivariate Data: Applications to PCA and PLS-DA of Omics Data.

    PubMed

    Saccenti, Edoardo; Timmerman, Marieke E

    2016-08-01

    Sample size determination is a fundamental step in the design of experiments. Methods for sample size determination are abundant for univariate analysis methods, but scarce in the multivariate case. Omics data are multivariate in nature and are commonly investigated using multivariate statistical methods, such as principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA). No simple approaches to sample size determination exist for PCA and PLS-DA. In this paper we will introduce important concepts and offer strategies for (minimally) required sample size estimation when planning experiments to be analyzed using PCA and/or PLS-DA.

  20. Hunting for the Tristan plume - An upper mantle tomography around the volcanic island Tristan da Cunha

    NASA Astrophysics Data System (ADS)

    Schlömer, Antje; Geissler, Wolfram H.; Jokat, Wilfried; Jegen, Marion

    2016-04-01

    Tristan da Cunha is a volcanic island in the South Atlantic close to the Mid-Atlantic Ridge. It is part of an area consisting of widely scattered seamounts and small islands at the western and youngest end of the aseismic Walvis Ridge. Tristan da Cunha together with the Walvis Ridge represents the classical example of a mantle plume track, because of the connection to the Cretaceous Etendeka flood basalt province in NW Namibia. The genesis of the island has so far remained enigmatic. It is hotly debated, if Tristan da Cunha sits actually above a deep mantle plume or if it is only originated by upwelling material from weak (leaky) fracture zones. It also has to be clarified if there are any indications for a plume-ridge interaction. Geochemical investigations have shown complex compositions of magmatic samples from Tristan da Cunha, which could be interpreted as a mixing of plume-derived melts and depleted upper mantle sources. To improve our understanding about the origin of Tristan and to test the mantle plume hypothesis, we deployed 24 broadband ocean-bottom seismometers and 2 seismological land stations around and on the island during an expedition in January 2012 with the German research vessel Maria S. Merian. After acquiring continuous seismological data for almost one year, the seismometers were recovered in early January 2013. We cross-correlated the arrival times of teleseismic P and PKP phases to perform a finite-frequency tomography of the upper mantle beneath the study area. Here we show the 3D mantle structure in terms of velocity variations: We do not image a "classical" plume-like structure directly beneath Tristan da Cunha, but we observe regions of low velocities at the edges of our array that we relate to local mantle upwelling from potentially deeper sources. Additionally we discuss local seismicity within the Tristan da Cunha region, which show processes along the nearby mid-ocean ridge and transform faults. Furthermore, the local seismicity

  1. O Imperio como Argumento: Um Contraponto entre Joaquim Nabuco e o Bispo D. Jose Mauricio da Rocha (The Empire as Argument: A Counterpoint between Joaquim Nabuco and the Bishop Dom Jose Mauricio da Rocha).

    ERIC Educational Resources Information Center

    Marson, Izabel Andrade

    1998-01-01

    Dialogs with the previous article establishing a comparative analysis between the political acceptance present in the reactionary speech of Dom Jose Mauricio da Rocha and in the monarchist words of Joaquim Nabuco. (PA)

  2. Knock-Down of the 37kDa/67kDa Laminin Receptor LRP/LR Impedes Telomerase Activity.

    PubMed

    Naidoo, Kerrilyn; Malindisa, Sibusiso T; Otgaar, Tyrone C; Bernert, Martin; Da Costa Dias, Bianca; Ferreira, Eloise; Reusch, Uwe; Knackmuss, Stefan; Little, Melvyn; Weiss, Stefan F T; Letsolo, Boitelo T

    2015-01-01

    Cancer has become a major problem worldwide due to its increasing incidence and mortality rates. Both the 37kDa/67kDa laminin receptor (LRP/LR) and telomerase are overexpressed in cancer cells. LRP/LR enhances the invasiveness of cancer cells thereby promoting metastasis, supporting angiogenesis and hampering apoptosis. An essential component of telomerase, hTERT is overexpressed in 85-90% of most cancers. hTERT expression and increased telomerase activity are associated with tumor progression. As LRP/LR and hTERT both play a role in cancer progression, we investigated a possible correlation between LRP/LR and telomerase. LRP/LR and hTERT co-localized in the perinuclear compartment of tumorigenic breast cancer (MDA_MB231) cells and non-tumorigenic human embryonic kidney (HEK293) cells. FLAG® Co-immunoprecipitation assays confirmed an interaction between LRP/LR and hTERT. In addition, flow cytometry revealed that both cell lines displayed high cell surface and intracellular LRP/LR and hTERT levels. Knock-down of LRP/LR by RNAi technology significantly reduced telomerase activity. These results suggest for the first time a novel function of LRP/LR in contributing to telomerase activity. siRNAs targeting LRP/LR may act as a potential alternative therapeutic tool for cancer treatment by (i) blocking metastasis (ii) promoting angiogenesis (iii) inducing apoptosis and (iv) impeding telomerase activity. PMID:26545108

  3. Identification of a major PAF acetylhydrolase in human serum/plasma as a 43 kDa glycoprotein containing about 9 kDa asparagine-conjugated sugar chain(s).

    PubMed

    Akiyama, M; Sugatani, J; Suzuki, T; Suzuki, Y; Miwa, M

    1998-05-01

    Platelet-activating factor (PAF) acetylhydrolase from human serum/plasma was identified on a polyvinylidene difluoride (PVDF) membrane by electroblotting proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme activity was detected in the 43 kDa region on the membrane as a decrease in the beta-radioluminescence of [3H]acetyl-PAF or by the convenient method for determining PAF acetylhydrolase activity (the TCA precipitation method). The enzyme activity on treatment with N-glycosidase F shifted to the 34 kDa region on the PVDF membrane. On the other hand, only one band was observed, corresponding to a molecular mass of 53 kDa, on analysis by SDS-PAGE with silver staining. Treatment of the 53 kDa protein with N-glycosidase F changed its molecular mass to 43 kDa (protein A). The NH2-terminal 32 amino acid sequence of protein A completely corresponds to that of the heterogenous enzyme with 54 amino acids deleted from the NH2 terminus reported by Tjoelker et al. (Nature 374, 549-553, 1995). Even after trypsin treatment of the N-glycosidase F-digested enzyme, its PAF-AH activity remained in the 34 kDa region, but the contaminating protein A disappeared, on the PVDF membrane. In addition, the majority of serum PAF-AH was retained on a Sambucus sieboldiana agglutinin (SSA)-agarose column and was eluted with the hapten sugar, lactose. These results indicate that PAF acetylhydrolase consisting of a 34 kDa protein and about 9 kDa asparagine-conjugated sugar chain(s) is a major enzyme in human serum/plasma. PMID:9562606

  4. A 9 kDa antifreeze protein from the Antarctic springtail, Gomphiocephalus hodgsoni.

    PubMed

    Hawes, T C; Marshall, C J; Wharton, D A

    2014-08-01

    A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techniques. Mass spectrometry identified a single 9 kDa component in the purified ice fraction. Intramolecular disulphide bonding was suggested by the presence of 12 cysteine residues. The specific amino acid composition is unique, particularly with regard to the presence of histidine (11.5%). But it also shows noticeable commonalities with insect AFPs in the abundance of cysteine (13.8%), while simultaneously hinting, through the presence of glycine (11.5%), that the metabolic building blocks of AFPs in Collembola may have a phylogenetically-determined component. PMID:25025820

  5. Crosslinking of hemin to a specific site on the 90-kDa ferritin repressor protein

    SciTech Connect

    Lin, Jihjing; Thach, R.E. ); Patino, M.M.; Gaffield, L.; Walden. W.E. ); Smith, A. )

    1991-07-15

    Incubation of a 90-kDa ferritin repressor protein (FRP) with small amounts of radiolabeled hemin resulted in the formation of a strong interaction between the two that was stable to SDS/PAGE. Of seven other proteins tested individually, only apohemopexin and bovine serum albumin showed similar crosslinking ability, albeit to a much lower extent. ({sup 14}C)Hemin specifically crosslinked to FRP in the presence of a 50-fold excess of total wheat germ proteins. Inclusion of catalase did not prevent the reaction of hemin with FRP, suggesting that H{sub 2}O{sub 2} is not involved. The subsequent addition of a stoichiometric amount of apohemopexin did not reverse the reaction. Exhaustive digestion of the complex with Staphylococcus aureus V8 protease produced a major labeled peptide of 17 kDa. These results show the existence of a highly specific, uniquely reactive hemin binding site on FRP.

  6. Towards the Implementation of an Autonomous Camera Algorithm on the da Vinci Platform.

    PubMed

    Eslamian, Shahab; Reisner, Luke A; King, Brady W; Pandya, Abhilash K

    2016-01-01

    Camera positioning is critical for all telerobotic surgical systems. Inadequate visualization of the remote site can lead to serious errors that can jeopardize the patient. An autonomous camera algorithm has been developed on a medical robot (da Vinci) simulator. It is found to be robust in key scenarios of operation. This system behaves with predictable and expected actions for the camera arm with respect to the tool positions. The implementation of this system is described herein. The simulation closely models the methodology needed to implement autonomous camera control in a real hardware system. The camera control algorithm follows three rules: (1) keep the view centered on the tools, (2) keep the zoom level optimized such that the tools never leave the field of view, and (3) avoid unnecessary movement of the camera that may distract/disorient the surgeon. Our future work will apply this algorithm to the real da Vinci hardware.

  7. [Interrelatio of acari Ixodidae and hosts of Edentata of the Serra da Canastra, Minas Gerais, Brazil].

    PubMed

    Botelho, J R; Linardi, P M; da Encarnação, C D

    1989-01-01

    We received for examination a small colection of ticks captured in the National Park of the Serra da Canastra (MG), between 1979 and 1980. The authors demonstrated the existence of a broad co-accomodation of Amblyomma pseudoconcolor on Edentata of the family Dasypodidae, being Dasypodini the tribe more adjusted to this infestation. In conformity to the Figs 1 and 2, Dasypodini are probably the real hosts of A. pseudoconcolor and also the oldest hosts. For the first time, A. pseudoconcolor is also recorded on Cabassous tatouay, C. unicinctus, Priodontes maximus and Euphractus sexcincuts. Also for the first time A. pseudoconcolor and Amblyomma calcaratum were recorded in the State of Minas Gerais. The ectoparasites are deposited in the "Departamento de Parasitologia da Universidade Federal de Minas Gerais, Brasil".

  8. From Jacobeaus to the da Vinci: thoracoscopic applications of the robot.

    PubMed

    Al-Mufarrej, Faisal; Margolis, Marc; Tempesta, Barbara; Strother, Eric; Najam, Farzad; Gharagozloo, Farid

    2010-02-01

    With the increasing recognition of the benefits of minimally invasive surgery, surgical technology has evolved significantly since Jacobeaus' first attempt at thoracoscopy 100 years ago. Currently, video-assisted thoracic surgery occupies a significant role in the diagnosis and treatment of benign and malignant diseases of the chest. However, the clinical application of video-assisted thoracic surgery is limited by the technical shortcomings of the approach. Although the da Vinci system (Intuitive Surgical) is not the first robotic surgical system, it has been the most successful and widely applicable. After early applications in general and urologic surgery, the da Vinci robot extended its arms into the field of thoracic surgery, broadening the applicability of minimally invasive thoracic surgery. We review the available literature on robot-assisted thoracic surgery in attempt to better define the current role of the robot in pulmonary, mediastinal, and esophageal surgeries.

  9. Iron abundance in the hot DA white dwarfs Feige 24 and G191 B2B

    NASA Technical Reports Server (NTRS)

    Vennes, Stephane; Chayer, Pierre; Thorstensen, John R.; Bowyer, Stuart; Shipman, Harry L.

    1992-01-01

    Attention is given to model calculations of the far- and extreme-UV line spectra of highly ionized Fe species (Fe IV, Fe V, and Fe VI) for hot high-gravity H-rich stars. A spectral analysis of 31 hr of exposure of the DA white dwarf Feige 24 with IUE in the echelle mode reveals the presence of Fe with an abundance relative to H by number of (5-10) x 10 exp -6 with an uncertainty dominated by the determination of stellar parameters. An analysis of IUE data from the white dwarf G191 B2B results in a similar Fe abundance if this star shares similar atmospheric parameters (Teff, g) with Feige 24. Fe is thus the second most abundant photospheric element in hot DA white dwarfs.

  10. Solid state of a new PDE-5 inhibitor DA-8159: characterization, dissolution, transformation.

    PubMed

    Ryu, Ji Yoon; Sohn, Young-Taek

    2012-05-01

    The polymorphic forms of a new PDE-5 inhibitor DA-8159 were prepared and characterized by differential scanning calorimetry (DSC), powder X-ray diffractometry (PXRD) and thermogravimetric analysis (TG). Two crystal forms and one amorphous form of DA-8159 have been isolated by recrystallization and characterized by DSC, TG and PXRD. From the TG data it was confirmed that two crystal forms are neither solvates nor hydrates. The PXRD patterns of the two crystal forms were different. In the dissolution studies in simulated intestinal fluid at 37 ± 0.5°C, the solubility decreased in the order of amorphous form > Form 1 > Form 2. After storage of 60 days, Form 1 was transformed to Form 2. Form 2 was not transformed. The amorphous form was transformed to Form 2 at 52% R.H. and 95% R.H., but it did not transform at 0% R.H. PMID:22644853

  11. Optimizing IrDA throughput by including processing time with physical layer consideration

    NASA Astrophysics Data System (ADS)

    Huang, Pi; Chatzimisios, P.; Boucouvalas, A. C.

    2005-06-01

    Feature Issue on Optical Wireless Communications (OWC) We derive new equations linking the Infrared Data Association (IrDA) data link layer (IrLAP) throughput to the physical layer by providing the bit error rate in relation to link distance and various physical layer parameters. Furthermore, we optimize the link by simultaneously adapting the frame and window size. The optimum equations include the effect of processor speed. The mathematical analysis is validated by comparison with exact numerical methods. Analytical results presented in the paper explore the effect of frame processing time on improving the performance of IrDA links and identify the improvement in throughput performance by utilizing the derived optimum values.

  12. Towards the Implementation of an Autonomous Camera Algorithm on the da Vinci Platform.

    PubMed

    Eslamian, Shahab; Reisner, Luke A; King, Brady W; Pandya, Abhilash K

    2016-01-01

    Camera positioning is critical for all telerobotic surgical systems. Inadequate visualization of the remote site can lead to serious errors that can jeopardize the patient. An autonomous camera algorithm has been developed on a medical robot (da Vinci) simulator. It is found to be robust in key scenarios of operation. This system behaves with predictable and expected actions for the camera arm with respect to the tool positions. The implementation of this system is described herein. The simulation closely models the methodology needed to implement autonomous camera control in a real hardware system. The camera control algorithm follows three rules: (1) keep the view centered on the tools, (2) keep the zoom level optimized such that the tools never leave the field of view, and (3) avoid unnecessary movement of the camera that may distract/disorient the surgeon. Our future work will apply this algorithm to the real da Vinci hardware. PMID:27046563

  13. Development of noSQL data storage for the ATLAS PanDA Monitoring System

    NASA Astrophysics Data System (ADS)

    Potekhin, M.; ATLAS Collaboration

    2012-06-01

    For several years the PanDA Workload Management System has been the basis for distributed production and analysis for the ATLAS experiment at the LHC. Since the start of data taking PanDA usage has ramped up steadily, typically exceeding 500k completed jobs/day by June 2011. The associated monitoring data volume has been rising as well, to levels that present a new set of challenges in the areas of database scalability and monitoring system performance and efficiency. These challenges are being met with a R&D effort aimed at implementing a scalable and efficient monitoring data storage based on a noSQL solution (Cassandra). We present our motivations for using this technology, as well as data design and the techniques used for efficient indexing of the data. We also discuss the hardware requirements as they were determined by testing with actual data and realistic loads.

  14. Visual degradation in Leonardo da Vinci's iconic self-portrait: A nanoscale study

    NASA Astrophysics Data System (ADS)

    Conte, A. Mosca; Pulci, O.; Misiti, M. C.; Lojewska, J.; Teodonio, L.; Violante, C.; Missori, M.

    2014-06-01

    The discoloration of ancient paper, due to the development of oxidized groups acting as chromophores in its chief component, cellulose, is responsible for severe visual degradation in ancient artifacts. By adopting a non-destructive approach based on the combination of optical reflectance measurements and time-dependent density functional theory ab-initio calculations, we describe and quantify the chromophores affecting Leonardo da Vinci's iconic self-portrait. Their relative concentrations are very similar to those measured in modern and ancient samples aged in humid environments. This analysis quantifies the present level of optical degradation of the Leonardo da Vinci's self-portrait which, compared with future measurements, will assess its degradation rate. This is a fundamental information in order to plan appropriate conservation strategies.

  15. Pressure effects on the Cl 2 (D'-A') transition at 258 nm

    NASA Astrophysics Data System (ADS)

    Nee, J. B.; Hubinger, S.

    1996-11-01

    UV emission at 258 nm from the transition of Cl 2(D'-A') was investigated by photoexcitation of the Rydberg states at pressures between 1 and 100 Torr. The intensity of the UV fluorescence produced was increased by a factor of two when the pressure of the buffer gas N 2 was increased from 1 to 20 Torr. Characteristic pressures for quenching and fluorescence enhancement were derived. Kinetic studies revealed the mechanism of the interstate transitions about the generation of the D'-A' transition. A vibrational state redistribution in the excitation spectra for the 2 1Σu+ state at high pressure was observed. The cause of this is explained in terms of state-dependent quenching.

  16. Surgical techniques: robot-assisted laparoscopic colposacropexy with the da Vinci(®) surgical system.

    PubMed

    Matthews, Catherine A

    2009-03-01

    Colposacropexy is the gold-standard operation for repair of apical vaginal support defects. While it is feasible to perform this operation using conventional laparoscopic techniques, a limited number of surgeons have mastered the advanced minimally invasive skills that are required. Introduction of the da Vinci(®) robotic system with instruments that have improved dexterity and precision and a camera system with three-dimensional imaging presents an opportunity for more surgeons treating women with pelvic organ prolapse to perform the procedure laparoscopically. This paper will outline a technique that is exactly modeled after the open procedure for completion of a robotic-assisted colposacropexy using the da Vinci(®) surgical system. PMID:27628451

  17. STRESS AND FAILURE ANALYSIS OF RAPIDLY ROTATING ASTEROID (29075) 1950 DA

    SciTech Connect

    Hirabayashi, Masatoshi; Scheeres, Daniel J.

    2015-01-01

    Rozitis et al. recently reported that near-Earth asteroid (29075) 1950 DA, whose bulk density ranges from 1.0 g cm{sup –3} to 2.4 g cm{sup –3}, is a rubble pile and requires a cohesive strength of at least 44-76 Pa to keep from failing due to its fast spin period. Since their technique for giving failure conditions required the averaged stress over the whole volume, it discarded information about the asteroid's failure mode and internal stress condition. This paper develops a finite element model and revisits the stress and failure analysis of 1950 DA. For the modeling, we do not consider material hardening and softening. Under the assumption of an associated flow rule and uniform material distribution, we identify the deformation process of 1950 DA when its constant cohesion reaches the lowest value that keeps its current shape. The results show that to avoid structural failure the internal core requires a cohesive strength of at least 75-85 Pa. It suggests that for the failure mode of this body, the internal core first fails structurally, followed by the surface region. This implies that if cohesion is constant over the whole volume, the equatorial ridge of 1950 DA results from a material flow going outward along the equatorial plane in the internal core, but not from a landslide as has been hypothesized. This has additional implications for the likely density of the interior of the body.

  18. 76 FR 19903 - Special Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel Cycle Engine

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ...These special conditions are issued for the Diamond Aircraft Industry (DAI) GmbH model DA-40NG the Austro Engine GmbH model E4 aircraft diesel engine (ADE) using turbine (jet) fuel. This airplane will have a novel or unusual design feature(s) associated with the installation of a diesel cycle engine utilizing turbine (jet) fuel. The applicable airworthiness regulations do not contain adequate......

  19. The Handedness of Leonardo da Vinci: A Tale of the Complexities of Lateralisation

    ERIC Educational Resources Information Center

    McManus, I. C.; Drury, Helena

    2004-01-01

    The handedness of Leonardo da Vinci is controversial. Although there is little doubt that many of his well-attributed drawings were drawn with the left hand, the hatch marks of the shading going downwards from left to right, it is not clear that he was a natural left-hander, there being some suggestion that he may have become left-handed as the…

  20. On the interpretation of the Exosat photometry of two hot DA white dwarfs

    NASA Technical Reports Server (NTRS)

    Vennes, Stephane; Shipman, Harry L.; Petre, Robert

    1990-01-01

    Exosat photometric observations of the hot DA white dwarfs GD 2 and EG 70 are presented and analyzed using model atmosphere and synthetic spectrum calculations. The numerical accuracy of the calculations and their impact on the reliability of the results are addressed. The optimum atmospheric parameters that explain the complete spectral coverage are derived. The consequences of these conjectures for the spectral evolution theory of white dwarf stars are considered.

  1. Diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT: a clinical follow up study.

    PubMed

    Menéndez-González, Manuel; Tavares, Francisco; Zeidan, Nahla; Salas-Pacheco, José M; Arias-Carrión, Oscar

    2014-01-01

    The [(123)I]ioflupane-a dopamine transporter radioligand-SPECT (DaT-SPECT) has proven to be useful in the differential diagnosis of tremor. Here, we investigate the diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT. Therefore, 30 patients with tremor and normal DaT-SPECT were followed up for 2 years. In 18 cases we were able to make a diagnosis. The residual 12 patients underwent a second DaT-SPECT, were then followed for additional 12 months and thereafter the diagnosis was reconsidered again. The final diagnoses included cases of essential tremor, dystonic tremor, multisystem atrophy, vascular parkinsonism, progressive supranuclear palsy, corticobasal degeneration, fragile X-associated tremor ataxia syndrome, psychogenic parkinsonism, iatrogenic parkinsonism and Parkinson's disease. However, for 6 patients the diagnosis remained uncertain. Larger series are needed to better establish the relative frequency of the different conditions behind these cases. PMID:24744729

  2. Diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT: a clinical follow up study

    PubMed Central

    Menéndez-González, Manuel; Tavares, Francisco; Zeidan, Nahla; Salas-Pacheco, José M.; Arias-Carrión, Oscar

    2014-01-01

    The [123I]ioflupane—a dopamine transporter radioligand—SPECT (DaT-SPECT) has proven to be useful in the differential diagnosis of tremor. Here, we investigate the diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT. Therefore, 30 patients with tremor and normal DaT-SPECT were followed up for 2 years. In 18 cases we were able to make a diagnosis. The residual 12 patients underwent a second DaT-SPECT, were then followed for additional 12 months and thereafter the diagnosis was reconsidered again. The final diagnoses included cases of essential tremor, dystonic tremor, multisystem atrophy, vascular parkinsonism, progressive supranuclear palsy, corticobasal degeneration, fragile X–associated tremor ataxia syndrome, psychogenic parkinsonism, iatrogenic parkinsonism and Parkinson's disease. However, for 6 patients the diagnosis remained uncertain. Larger series are needed to better establish the relative frequency of the different conditions behind these cases. PMID:24744729

  3. Formation of P-Rich Olivine in DaG 978 Carbonaceous Chondrite Through Fluid-Assisted Metamorphism

    NASA Astrophysics Data System (ADS)

    Zhang, A. C.; Li, Y.; Chen, J. N.; Gu, L. X.; Wang, R. C.

    2016-08-01

    We describe an occurrence of P-rich olivine in the DaG 978 carbonaceous chondrite. Different from other natural P-rich olivine, the P-rich olivine should have formed through fluid-assisted metamorphism.

  4. Caulis Sinomenii extracts activate DA/NE transporter and inhibit 5HT transporter.

    PubMed

    Zhao, Gang; Bi, Cheng; Qin, Guo-Wei; Guo, Li-He

    2009-08-01

    Caulis Sinomenii (QFT) has analgesic, sedative, and anxiolytic-like actions, and is proven effective for improving drug dependence that is known to be associated with abnormal monoaminergic transmission. We assessed whether QFT would be biologically active in functionally regulating monoamine transporters using CHO cells expressing dopamine transporter (DAT), norepinephrine transporter (NET), or serotonin transporter (SERT) (i.e. D8, N1, or S6 cells, respectively). Here, we showed that its primary extracts, such as QA, QC, QE, QD, and QB (QFT ethanol, chloroform, ethyl acetate, alkaloid-free chloroform, and alkaloid-containing chloroform extract, respectively), and secondary extracts, such as QE-2, - 3, - 5, - 7, QD-1, - 2, - 3, - 4, - 5, and QB-1, - 2, - 3, - 4, - 5 (fractioned from QE, QD, and QB, respectively), in differing degrees, either increased DA/ NE uptake by corresponding D8/N1 cells or decreased 5HT uptake by S6 cells; wherein, QE-2, QD-3, and QE-7 were potent DA/NE uptake activators while both QE-7 and QB-5 were potent 5HT uptake inhibitors. Furthermore, the enhancement of DA/NE uptake was dependent of DAT/NET activity, and the inhibition of 5HT uptake was typical of competition. Thus, QFT extracts, especially QE-2 and QE-7 (both with stronger potencies), are novel monoamine transporter modulators functioning as DAT/ NET activators and/or SERT inhibitors, and would likely improve neuropsychological disorders through regulating monoamine transporters.

  5. OnDA: online data analysis and feedback for serial X-ray imaging1

    PubMed Central

    Mariani, Valerio; Morgan, Andrew; Yoon, Chun Hong; Lane, Thomas J.; White, Thomas A.; O’Grady, Christopher; Kuhn, Manuela; Aplin, Steve; Koglin, Jason; Barty, Anton; Chapman, Henry N.

    2016-01-01

    This article describes a free and open-source data analysis utility designed for fast online feedback during serial X-ray diffraction and scattering experiments: OnDA (online data analysis). Three complete real-time monitors for common types of serial X-ray imaging experiments are presented. These monitors are capable of providing the essential information required for quick decision making in the face of extreme rates of data collection. In addition, a set of modules, functions and algorithms that allow developers to modify the provided monitors or develop new ones are provided. The emphasis here is on simple, modular and scalable code that is based on open-source libraries and protocols. OnDA monitors have already proven to be invaluable tools in several experiments, especially for scoring and monitoring of diffraction data during serial crystallography experiments at both free-electron laser and synchrotron facilities. It is felt that in the future the kind of fast feedback that OnDA monitors provide will help researchers to deal with the expected very high throughput data flow at next-generation facilities such as the European X-ray free-electron laser. PMID:27275150

  6. da Vinci robot-assisted keyhole neurosurgery: a cadaver study on feasibility and safety.

    PubMed

    Marcus, Hani J; Hughes-Hallett, Archie; Cundy, Thomas P; Yang, Guang-Zhong; Darzi, Ara; Nandi, Dipankar

    2015-04-01

    The goal of this cadaver study was to evaluate the feasibility and safety of da Vinci robot-assisted keyhole neurosurgery. Several keyhole craniotomies were fashioned including supraorbital subfrontal, retrosigmoid and supracerebellar infratentorial. In each case, a simple durotomy was performed, and the flap was retracted. The da Vinci surgical system was then used to perform arachnoid dissection towards the deep-seated intracranial cisterns. It was not possible to simultaneously pass the 12-mm endoscope and instruments through the keyhole craniotomy in any of the approaches performed, limiting visualization. The articulated instruments provided greater dexterity than existing tools, but the instrument arms could not be placed in parallel through the keyhole craniotomy and, therefore, could not be advanced to the deep cisterns without significant clashing. The da Vinci console offered considerable ergonomic advantages over the existing operating room arrangement, allowing the operating surgeon to remain non-sterile and seated comfortably throughout the procedure. However, the lack of haptic feedback was a notable limitation. In conclusion, while robotic platforms have the potential to greatly enhance the performance of transcranial approaches, there is strong justification for research into next-generation robots, better suited to keyhole neurosurgery.

  7. Regolith grain size and cohesive strength of near-Earth Asteroid (29075) 1950 DA

    NASA Astrophysics Data System (ADS)

    Gundlach, B.; Blum, J.

    2015-09-01

    Due to its fast rotation period of 2.12 h, about half of the surface of near-Earth Asteroid (29075) 1950 DA experiences negative (i.e., outward directed) acceleration levels (Rozitis, B., Maclennan, E., Emery, J.P. [2014]. Nature 512, 174-176). Thus, cohesion of the surface material is mandatory to prevent rotational breakup of the asteroid. Rozitis et al. (Rozitis, B., Maclennan, E., Emery, J.P. [2014]. Nature 512, 174-176) concluded that a grain size of ∼6 cm or lower is needed to explain the required cohesive strength of 64-20+12Pa . Here, we present another approach to determine the grain size of near-Earth Asteroid (29075) 1950 DA by using the thermal inertia value from Rozitis et al. (Rozitis, B., Maclennan, E., Emery, J.P. [2014]. Nature 512, 174-176) and a model of the heat conductivity of the surface regolith (Gundlach, B., Blum, J. [2013]. Icarus 223, 479-492). This method yields a mean particle radius ranging from 32 μm to 117 μm. The derived grain sizes are then used to infer the cohesive strength of the surface material of Asteroid (29075) 1950 DA (ranging from 24 Pa to 88 Pa), by using laboratory measurements of the tensile strength of powders.

  8. [History of robotics: from archytas of tarentum until Da Vinci robot. (Part II)].

    PubMed

    Sánchez-Martín, F M; Jiménez Schlegl, P; Millán Rodríguez, F; Salvador-Bayarri, J; Monllau Font, V; Palou Redorta, J; Villavicencio Mavrich, H

    2007-03-01

    Robotic surgery is a reality. In order to to understand how new robots work is interesting to know the history of ancient (see part i) and modern robotics. The desire to design automatic machines imitating humans continued for more than 4000 years. Archytas of Tarentum (at around 400 a.C.), Heron of Alexandria, Hsieh-Fec, Al-Jazari, Bacon, Turriano, Leonardo da Vinci, Vaucanson o von Kempelen were robot inventors. At 1942 Asimov published the three robotics laws. Mechanics, electronics and informatics advances at XXth century developed robots to be able to do very complex self governing works. At 1985 the robot PUMA 560 was employed to introduce a needle inside the brain. Later on, they were designed surgical robots like World First, Robodoc, Gaspar o Acrobot, Zeus, AESOP, Probot o PAKI-RCP. At 2000 the FDA approved the da Vinci Surgical System (Intuitive Surgical Inc, Sunnyvale, CA, USA), a very sophisticated robot to assist surgeons. Currently urological procedures like prostatectomy, cystectomy and nephrectomy are performed with the da Vinci, so urology has become a very suitable speciality to robotic surgery.

  9. da Vinci robot-assisted keyhole neurosurgery: a cadaver study on feasibility and safety.

    PubMed

    Marcus, Hani J; Hughes-Hallett, Archie; Cundy, Thomas P; Yang, Guang-Zhong; Darzi, Ara; Nandi, Dipankar

    2015-04-01

    The goal of this cadaver study was to evaluate the feasibility and safety of da Vinci robot-assisted keyhole neurosurgery. Several keyhole craniotomies were fashioned including supraorbital subfrontal, retrosigmoid and supracerebellar infratentorial. In each case, a simple durotomy was performed, and the flap was retracted. The da Vinci surgical system was then used to perform arachnoid dissection towards the deep-seated intracranial cisterns. It was not possible to simultaneously pass the 12-mm endoscope and instruments through the keyhole craniotomy in any of the approaches performed, limiting visualization. The articulated instruments provided greater dexterity than existing tools, but the instrument arms could not be placed in parallel through the keyhole craniotomy and, therefore, could not be advanced to the deep cisterns without significant clashing. The da Vinci console offered considerable ergonomic advantages over the existing operating room arrangement, allowing the operating surgeon to remain non-sterile and seated comfortably throughout the procedure. However, the lack of haptic feedback was a notable limitation. In conclusion, while robotic platforms have the potential to greatly enhance the performance of transcranial approaches, there is strong justification for research into next-generation robots, better suited to keyhole neurosurgery. PMID:25516094

  10. Attachment of group B streptococci to macrophages is mediated by a 21-kDa protein.

    PubMed

    Smith, L M; Laganas, V; Pistole, T G

    1998-02-01

    Group B Streptococcus (GBS) is able to bind to human macrophages in vitro in the absence of exogenous opsonins. The exact mechanisms that mediate this attachment are unclear. This study was undertaken to determine what protein adhesins are present on the surface of GBS that mediate attachment to macrophages. We have identified a 21-kDa protein from the envelope of GBS type III that directly binds to macrophages as determined by Western blot analysis. Antiserum against this protein was able to inhibit binding of GBS to macrophages by greater than 80% as measured by flow cytometry. Antiserum against the 21-kDa protein cross-reacted with 21-kDa proteins from GBS type Ib, type II, type III (COH31 and MR732) and type IV, as well as Staphyloccus epidermidis, but not GBS type Ia, Listeria monocytogenes or Enterococcus faecalis. This protein may be important in mediating the attachment of GBS to macrophages in an opsonin-poor environment.

  11. Co-activation of VTA DA and GABA neurons mediates nicotine reinforcement.

    PubMed

    Tolu, S; Eddine, R; Marti, F; David, V; Graupner, M; Pons, S; Baudonnat, M; Husson, M; Besson, M; Reperant, C; Zemdegs, J; Pagès, C; Hay, Y A H; Lambolez, B; Caboche, J; Gutkin, B; Gardier, A M; Changeux, J-P; Faure, P; Maskos, U

    2013-03-01

    Smoking is the most important preventable cause of mortality and morbidity worldwide. This nicotine addiction is mediated through the nicotinic acetylcholine receptor (nAChR), expressed on most neurons, and also many other organs in the body. Even within the ventral tegmental area (VTA), the key brain area responsible for the reinforcing properties of all drugs of abuse, nicotine acts on several different cell types and afferents. Identifying the precise action of nicotine on this microcircuit, in vivo, is important to understand reinforcement, and finally to develop efficient smoking cessation treatments. We used a novel lentiviral system to re-express exclusively high-affinity nAChRs on either dopaminergic (DAergic) or γ-aminobutyric acid-releasing (GABAergic) neurons, or both, in the VTA. Using in vivo electrophysiology, we show that, contrary to widely accepted models, the activation of GABA neurons in the VTA plays a crucial role in the control of nicotine-elicited DAergic activity. Our results demonstrate that both positive and negative motivational values are transmitted through the dopamine (DA) neuron, but that the concerted activity of DA and GABA systems is necessary for the reinforcing actions of nicotine through burst firing of DA neurons. This work identifies the GABAergic interneuron as a potential target for smoking cessation drug development.

  12. Enhanced efficacy and immunogenicity of 78kDa antigen formulated in various adjuvants against murine visceral leishmaniasis.

    PubMed

    Nagill, Rajeev; Kaur, Sukhbir

    2010-05-21

    Leishmania infection causes localized cutaneous to severe visceral disease in humans and animals. Current control measures, based on antimonial compounds, are not effective because of resistance in Leishmania. Vaccination would be a feasible alternative, but as yet no vaccine to protect humans against infection has been commercialized. Parasite antigens that preferentially stimulate the induction of significant protection through Th1 response presents a rational approach for a vaccine against leishmaniasis. With this view in mind, we investigated the potential of 78kDa antigen of Leishmania donovani alone and along with different adjuvants against murine visceral leishmaniasis. Various adjuvants used along with 78kDa antigen include monophosphoryl lipid A (MPL-A), liposomal encapsulation, recombinant IL-12, autoclaved Leishmania antigen (ALD) and Freund's adjuvant (FCA). BALB/c mice were immunized subcutaneously thrice with respective vaccine formulation. Challenge infection was given intracardially after 2 weeks of second booster. A significant decrease in parasite burden was seen in vaccinees over the infected controls on all post challenge days and was found that maximum protection was provided by 78kDa+rIL-12 vaccine and it was highly immunogenic as depicted by the reduction in parasite load (71-94.8%), reduction in infection rate of peritoneal macrophages (92.9-98%), enhanced DTH response (6.5-10.5 fold), increase in IgG2a anti-leishmanial antibody production (3-3.7 fold) and up-regulation of IFN-gamma (3.7-6.5 fold) and IL-2 levels (7.7-12.3 fold), which demonstrate the generation of protective Th1 type of immune response. Comparable results were also observed in 78kDa+MPL-A and liposome-encapsulated 78kDa vaccines with 56.5-92% and 62.9-93.4% reduction in parasite load respectively. Significant results have also been obtained with 78kDa antigen+ALD, 78kDa antigen+FCA and 78kDa antigen alone group but the protective efficacy was reduced as compared to the

  13. 32 CFR Appendix D to Part 623 - Certificate for Signature by an Alternate (DA Form 4881-1-R)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 3 2014-07-01 2014-07-01 false Certificate for Signature by an Alternate (DA Form 4881-1-R) D Appendix D to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. D Appendix D to Part 623—Certificate for Signature by an Alternate (DA...

  14. 32 CFR Appendix D to Part 623 - Certificate for Signature by an Alternate (DA Form 4881-1-R)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 3 2013-07-01 2013-07-01 false Certificate for Signature by an Alternate (DA Form 4881-1-R) D Appendix D to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. D Appendix D to Part 623—Certificate for Signature by an Alternate (DA...

  15. 32 CFR Appendix C to Part 623 - Agreement for Loan of US Army Materiel (DA Form 4881-R)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 3 2012-07-01 2009-07-01 true Agreement for Loan of US Army Materiel (DA Form 4881-R) C Appendix C to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. C Appendix C to Part 623—Agreement for Loan of US Army Materiel (DA Form...

  16. 32 CFR Appendix C to Part 623 - Agreement for Loan of US Army Materiel (DA Form 4881-R)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 3 2013-07-01 2013-07-01 false Agreement for Loan of US Army Materiel (DA Form 4881-R) C Appendix C to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. C Appendix C to Part 623—Agreement for Loan of US Army Materiel (DA Form...

  17. 32 CFR Appendix C to Part 623 - Agreement for Loan of US Army Materiel (DA Form 4881-R)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 3 2014-07-01 2014-07-01 false Agreement for Loan of US Army Materiel (DA Form 4881-R) C Appendix C to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. C Appendix C to Part 623—Agreement for Loan of US Army Materiel (DA Form...

  18. 32 CFR Appendix D to Part 623 - Certificate for Signature by an Alternate (DA Form 4881-1-R)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 3 2012-07-01 2009-07-01 true Certificate for Signature by an Alternate (DA Form 4881-1-R) D Appendix D to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. D Appendix D to Part 623—Certificate for Signature by an Alternate (DA Form...

  19. 32 CFR Appendix C to Part 623 - Agreement for Loan of US Army Materiel (DA Form 4881-R)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Agreement for Loan of US Army Materiel (DA Form... ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. C Appendix C to Part 623—Agreement for Loan of US Army Materiel (DA Form 4881-R) EC24OC91.013 EC24OC91.014 EC24OC91.015 EC24OC91.016...

  20. 32 CFR Appendix C to Part 623 - Agreement for Loan of US Army Materiel (DA Form 4881-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Agreement for Loan of US Army Materiel (DA Form... ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. C Appendix C to Part 623—Agreement for Loan of US Army Materiel (DA Form 4881-R) EC24OC91.013 EC24OC91.014 EC24OC91.015 EC24OC91.016...

  1. A 33 kDa molecular marker of sperm acrosome differentiation and maturation in the tammar wallaby (Macropus eugenii).

    PubMed

    Wade, M A; Lin, M

    1999-09-01

    This study was undertaken to identify potential molecular markers of acrosomal biogenesis and post-testicular maturation in marsupials, using the tammar wallaby as a model species. A two-step sperm extraction procedure yielded two protein extracts of apparent acrosomal origin and a tail extract. The extracts were analysed by SDS-PAGE under reducing conditions. Several prominent polypeptide bands (45, 38 and 33 kDa) appeared common to both acrosomal extracts. Antiserum raised against the 33 kDa polypeptide from the inner acrosomal membrane matrix (IAMM) extract showed immunoreactivity with 45, 38 and 33 kDa polypeptides in both acrosomal extracts, indicating that the 33 kDa polypeptide was related to the proteins in the 45 and 38 kDa bands. Therefore, the antiserum was used as a molecular probe. Indirect immuno-fluorescence indicated that the acrosome was the major location of the 33 kDa polypeptide. This contention was confirmed by ultrastructural study: immunogold labelling indicated that the 33 kDa polypeptide associated with acrosomal matrix components throughout acrosomal development in the testes and throughout post-testicular maturation in the epididymis. The label clearly delineated the changing morphology of the maturing marsupial acrosome. This is the first study to use immunocytochemical techniques to chart testicular and post-testicular development of any sperm organelle in a marsupial. As a result of this study, a 33 kDa molecular marker of marsupial acrosome differentiation and maturation has been identified. It may be possible to chart similar events in other marsupial species and identify opportunities for manipulating fertility. PMID:10645248

  2. Espectroscopia multi-objeto e imageamento de Abell 586 com GMOS - o estado dinâmico de um aglomerado de galáxias com arcos gravitacionais

    NASA Astrophysics Data System (ADS)

    Cypriano, E. S.; Sodrã©, L., Jr.; Kneib, J.-P.; Campusano, L.

    2003-08-01

    Nesse trabalho é apresentado um estudo do conteúdo de massa do aglomerado de galáxias A586 (z = 0.171) bem como sua distribuição espacial. Isso foi feito a partir de dados de espectroscopia multi-objeto e imageamento obtidos com o instrumento GMOS acoplado ao telescópio Gemini-Norte. A massa desse aglomerado foi estudada a partir da posição dos seus arcos gravitacionais (lentes fortes), da medida estatística da distorção na forma das galáxias de fundo (lentes fracas) e através da dispersão de velocidades de uma amostra de cerca de 30 galáxias pertencentes ao aglomerado (teorema do virial). Os dois primeiros métodos não dependem do estado dinâmico do aglomerado, enquanto que o terceiro sim. Desse modo, comparando seus resultados pode-se estimar o grau de relaxamento desse sistema. Dados de raios X presentes na literatura também serão incluídos nessa análise. Nossos resultados preliminares a partir das técnicas de lentes fracas, mostram que, supondo que a distribuição de massa desse aglomerado seja a de uma esfera isotérmica, espera-se que ele apresente uma dispersão de velocidades de 1330+/-78 km s-1, em contradicao com medidas similares feitas por Dahle et al. 2002 que encontram valores tão altos quanto 1680 km s-1. Já o estudo dinâmico resultou numa dispersão de velocidades de ~950 km s-1.

  3. Effect of DA-8159, a Selective Phosphodiesterase Type 5 Inhibitor, on Electroretinogram and Retinal Histology in Rabbits

    PubMed Central

    Kang, Kyung Koo; Ahn, Gook Jun; Shim, Hyun Joo; Kim, Won Bae

    2004-01-01

    DA-8159, a selective inhibitor of phosphodiesterase type 5, was developed as a new drug for erectile dysfunction. The effect of DA-8159 on the electroretinogram (ERG) and the retinal histopathology were evaluated in rabbits. The ERG was performed prior to, and 1 and 5 hr after DA-8159 (5 to 30 mg/kg) administration. The plasma concentration of DA-8159 was determined at each time point, and retinal microscopic examination was also performed. There was no statistically significant ERG change at any dose or at any time. Though the 30 Hz flicker showed a prolongation of the implicit time at 5 hr after the administration of either DA-8159 15 mg or 30 mg/kg (p<0.05), but concurrent amplitude decreases were not statistically significant. At a dose of 5 mg/kg, no test drug was detected in the blood after either 1 or 5 hr. At either 15 mg/kg or 30 mg/kg, there was a dose-dependent increase in the blood concentration after 1 hr of drug administration, which decreased with time. In light and electron microscopic examinations of the retina, there was no remarkable change at any dose. These results suggest DA-8159 has a low risk potential to the retina, but further evaluation on the visual functions in human is needed. PMID:15308852

  4. The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs.

    PubMed Central

    Takagaki, Y; MacDonald, C C; Shenk, T; Manley, J L

    1992-01-01

    Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-crosslinked to RNA in a polyadenylylation signal (AAUAAA)-dependent manner. We have now isolated cDNAs encoding the 64-kDa subunit of human cleavage stimulation factor. The 64-kDa subunit contains a ribonucleoprotein-type RNA binding domain in the N-terminal region and a repeat structure in the C-terminal region in which a pentapeptide sequence (consensus MEARA/G) is repeated 12 times and the formation of a long alpha-helix stabilized by salt bridges is predicted. An approximately 270-amino acid segment surrounding this repeat structure is highly enriched in proline and glycine residues (approximately 20% for each). When cloned 64-kDa subunit was expressed in Escherichia coli, an N-terminal fragment containing the RNA binding domain bound to RNAs in a polyadenylylation-signal-independent manner, suggesting that the RNA binding domain is directly involved in the binding of the 64-kDa subunit to pre-mRNAs. Images PMID:1741396

  5. [Clinical curative efficacy of inducing remission for the newly diagnosed aged AML patients by chemotherapy with IA and DA regimens].

    PubMed

    Tian, Dong-Hua; Gan, Si-Lin; Xing, Hai-Zhou; Liu, Yan-Fang; Xie, Xin-Sheng; Sun, Hui

    2014-10-01

    This study was aimed to explore the clinical efficacy and toxicity of idarubicin (IA regimen) and daunoru-bicin combined with cytarabine (DA regimen) for treating aged patients with AML as induction chemotherapy. The clinical data of 60 newly diagnosed AML aged patients treated with IA or DA regimen were analyzed retrospectively. IA regimen group included 22 patients (8 male and 14 females with median age of 66 yrs), while the DA regimen group included 38 patients (20 males and 18 females with median age of 64 yrs). The complete remission rate, total effective rate and adverse effects after one chemotherapy course were compared. The results showed that the CR rate in IA regimen group was 63.63%, which was significantly higer than that in DA regimen group (31.58%) (P < 0.05). The total effective rate was 63.63% and 36.84% respectively in IA and DA regimen groups, there was significant difference between the two groups (P < 0.05). Both the hematological and non-hematological adverse effects were observed and no difference was found in the two regimen groups, neither in myelosupression (P > 0.05), the major hematological adverse effects, nor in non-hematological adverse effects (P > 0.05). It is concluded that for aged AML patients, IA regimen can achieve a higher CR rate and higher total effective rate than that in DA regimen without increase of adverse effects after one induction chemotherapy course.

  6. Histopathological effects and determination of the putative receptor of Bacillus thuringiensis Cry1Da toxin in Spodoptera littoralis midgut.

    PubMed

    BenFarhat-Touzri, Dalel; Saadaoui, Marwa; Abdelkefi-Mesrati, Lobna; Saadaoui, Imen; Azzouz, Hichem; Tounsi, Slim

    2013-02-01

    Bacillus thuringiensis subsp. aizawai strain HD133, known by its effectiveness against Spodoptera species, produces many insecticidal proteins including Cry1Ab, Cry1Ca and Cry1Da. In the present study, the insecticidal activity of Cry1Da against Spodoptera littoralis was investigated. It showed toxicity with an LC(50) of 224.4 ng/cm(2) with 95% confidence limits of (178.61-270.19) and an LC(90) of 467.77 ng/cm(2) with 95% confidence limits of (392.89-542.65). The midgut histopathology of Cry1Da fed larvae showed vesicle formation in the apical region, vacuolization and destruction of epithelial cells. Biotinylated-activated Cry1Da toxin bound protein of about 65 kDa on blots of S. littoralis brush border membrane preparations. This putative receptor differs in molecular size from those recognized by Cry1C and Vip3A which are active against this polyphagous insect. This difference in midgut receptors strongly supports the use of Cry1Da as insecticidal agent, particularly in case of Cry and/or Vip-resistance management. PMID:23220238

  7. OpenDA Open Source Generic Data Assimilation Environment and its Application in Process Models

    NASA Astrophysics Data System (ADS)

    El Serafy, Ghada; Verlaan, Martin; Hummel, Stef; Weerts, Albrecht; Dhondia, Juzer

    2010-05-01

    Data Assimilation techniques are essential elements in state-of-the-art development of models and their optimization with data in the field of groundwater, surface water and soil systems. They are essential tools in calibration of complex modelling systems and improvement of model forecasts. The OpenDA is a new and generic open source data assimilation environment for application to a choice of physical process models, applied to case dependent domains. OpenDA was introduced recently when the developers of Costa, an open-source TU Delft project [http://www.costapse.org; Van Velzen and Verlaan; 2007] and those of the DATools from the former WL|Delft Hydraulics [El Serafy et al 2007; Weerts et al. 2009] decided to join forces. OpenDA makes use of a set of interfaces that describe the interaction between models, observations and data assimilation algorithms. It focuses on flexible applications in portable systems for modelling geophysical processes. It provides a generic interfacing protocol that allows combination of the implemented data assimilation techniques with, in principle, any time-stepping model duscribing a process(atmospheric processes, 3D circulation, 2D water level, sea surface temperature, soil systems, groundwater etc.). Presently, OpenDA features filtering techniques and calibration techniques. The presentation will give an overview of the OpenDA and the results of some of its practical applications. Application of data assimilation in portable operational forecasting systems—the DATools assimilation environment, El Serafy G.Y., H. Gerritsen, S. Hummel, A. H. Weerts, A.E. Mynett and M. Tanaka (2007), Journal of Ocean Dynamics, DOI 10.1007/s10236-007-0124-3, pp.485-499. COSTA a problem solving environment for data assimilation applied for hydrodynamical modelling, Van Velzen and Verlaan (2007), Meteorologische Zeitschrift, Volume 16, Number 6, December 2007 , pp. 777-793(17). Application of generic data assimilation tools (DATools) for flood

  8. [Analysis of algal blooms in Da-Ning River of Three Gorges Reservoir].

    PubMed

    Zheng, Bing-Hui; Cao, Cheng-Jin; Zhang, Jia-Lei; Huang, Min-Sheng; Chen, Zhen-Lou

    2009-11-01

    According to the survey conducted from Apr. to Jun. 2007 and from Apr. to May. 2008, the changes of water quality, forms and distributions of nutrient salts and characters of algal blooms in Da-ning River of Three Gorges Reservoir (TGR) were studied. The results indicated that the concentrations of nitrogen and phosphorus nutrient were abundant during sensitive period of algal blooms in Da-ning River. Total nitrogen (TN) and total phosphorus (TP) values are 0.84-3.21 mg/L and 0.011-0.531 mg/L respectively, and the nutrients concentrations become high gradually from upstream to downstream. Total dissolved nitrogen (TDN) is the major form of TN accounting for 84%, and total dissolved phosphorus (TDP) is dominant (TDP/TP = 60%). Algal blooms bring phosphorus nutrient bio-concentration. The rates of TN and TP are all in excess of 16, which show eutrophication is limited by phosphorus. Potassium permanganate index and dissolved oxygen (DO) are at low levels and change stably. But chlorophyll a (Chl-a) becomes frequently, the value is 1.41-219.04 mg x m(-3). Significant positive correlations are all observed by correlation analysis between Chl-a and the main parameters (r(Chla-TP) = 0.453, r(Chla-potassium permanganate index) = 0.641, r(Chla-DO) = 0.584, r(Chla-pH) = 0.409, p < 0.01), but significant negative correlations are observed between Chl-a and Secchi depth (SD) (r(Chla-SD) = - 0.392, p < 0.01). The pH is fluctuated by multiparameter esp. in algal blooms. Widespread algae are observed by microscope during sensitive period of algal blooms in Da-ning River accounting for 8 phylum 82 genus 124 species, which Bacillariophyta and Chlorophyta are dominant, and then Cyanophyta and Pyrrophyta. Three whole watershed algal blooms break out in Da-ning River during the period, and the highest values of algal density are 14-1 427 times as many as the normal values. The dominant species of algal blooms are mostly involved with O. borgei, C. microporum, Chlorococcum humicola, P

  9. The Near-Earth Flyby of Asteroid 2012 DA14 (Invited)

    NASA Astrophysics Data System (ADS)

    Moskovitz, N. A.; Endicott, T.; Lister, T.; Ryan, B.; Ryan, E. V.; Willman, M.; Hergenrother, C.; Binzel, R. P.; Polishook, D.; DeMeo, F.; Bennechi, S.; Sheppard, S.; Marchis, F.; Angusteijn, T.; Birthwhistle, P.; Verveer, A.; Gulbis, A.; Nagayama, T.; Gilmore, A.; Kilmartin, P.

    2013-12-01

    On UT 2013 February 15, the elongated 20x40 meter near-Earth asteroid 2012 DA14 made a close flyby of the Earth at a distance of approximately 27,000 km or 4 Earth-radii. This passage was inside the orbital distance of geosynchronous satellites and close to the Earth's Roche limit. This close encounter was unprecedented in our advance knowledge of the incoming trajectory, thereby allowing us to forge a detailed coordinated plan for observation and testing of physical models predicting tidal effects near our planet's Roche limit. Predicted outcomes of such close encounters include tidal disruption, changes in rotation state, and mass loss or mass redistribution due to seismic shaking. Each of these effects sensitively depend on how tidal energy is dissipated in the interior of the asteroid: a rubble pile will dissipate tidal energy differently than a solid or partially fractured monolith. The internal properties of asteroids in general and particularly those in the size range of 2012 DA14 are largely unknown. This encounter represents one of the first ever opportunities to observationally test models of the interior structure of small planetary bodies. We will present the results of a large multi-observatory campaign focused on 2012 DA14. This campaign includes data from shortly after discovery in 2012 and surrounding the close approach of the asteroid in February of 2013. These data were collected with over a dozen telescopes worldwide, ranging in aperture from 1m to 8m and include major facilities in Chile, Australia, New Zealand, South Africa, Israel, Hawaii, Arizona, New Mexico, and the Canary Islands. We will highlight the outcome of pre- and post-flyby visible-wavelength photometric and spectroscopic observations. This will include clear evidence that 2012 DA14 is now in a non-principal axis rotation state, requiring a multi-periodic tumbling model to fit its post-flyby rotational light curve. Such a complex rotation state is consistent with models for the

  10. The Greek Database of Seismogenic Sources (GreDaSS): the new version.

    NASA Astrophysics Data System (ADS)

    Caputo, Riccardo; Pavlides, Spyros; GreDaSS Working Group

    2014-05-01

    The Greek Database of Seismogenic Sources (GreDaSS) is a repository of geological, tectonic and active fault data for the Greek territory and its surroundings including sectors of western Anatolia, southern Bulgaria, FYR of Macedonia and Albania. The principal aim of this international project is to create a homogenized framework of all data relevant to the seismotectonics, and especially the seismic hazard assessment, of Greece and its surroundings as well as a common research platform for performing seismic hazard analyses, modelling and scenarios from specific seismogenic structures. GreDaSS represents an on-going project based on the results of decades of investigations by the GreDaSS Working Group* and by numerous other researchers working on the active tectonics of the broader Aegean Region. Members of the WG have contributed in several national and European projects like FAUST (Faults as a seismological tool, 1998-2000) and SHARE (Seismic Hazard and Armonization in Europe, 2009-2012). The database is continuously updated by the WG, with the aim of improving its completeness in terms of seismogenic sources analysed and included, but also of enriching and better defining all parametric and ancillary information on each source. Preliminary and partial versions of GreDaSS have been already presented at previous congresses (Pavlides et al., 2010; Caputo et al., 2012; Sboras et al., 2013), but at next EGU the whole area and the most recent version of the database will be displayed. *) the principal scientific contributors belonging to the GreDaSS WG are: Sboras. S., Chatzipetros A., Koukouvelas I., Michailidou A., Valkaniotis S., Tarabusi G. Caputo R., Chatzipetros A., Pavlides S. and Sboras S. (2012): Ann. Geophysics, 55(5), 859-894, doi: 10.4401/ag-5168 Pavlides S., Caputo R., Sboras S., Chatzipetros A., Papathanasiou G. & Valkaniotis S. (2010): Bull. Geol. Soc. Greece, 43(1), 486-494 Sboras S., Pavlides S., Caputo R., Chatzipetros A., Michailidou A

  11. Variação temporal do gradiente radial de o/h a partir de uma amostra de nebulosas planetárias

    NASA Astrophysics Data System (ADS)

    Maciel, W. J.; Costa, R. D. D.; Uchida, M. M. M.

    2003-08-01

    Gradientes radiais de abundância são observados no disco de nossa Galáxia e também em outras galáxias espirais. No caso da Galáxia, o principal problema relativo aos gradientes é sua variação temporal, que constitui um dos principais vínculos aos modelos de evolução química da Galáxia. As nebulosas planetárias são particularmente interessantes no estudo dos gradientes e suas variações, tanto espaciais como temporais. São objetos brilhantes, podendo ser observadas a grandes distâncias, e têm abundâncias relativamente precisas de diversos elementos químicos. Além disso, sendo originadas de estrelas com massas entre 0.8 e 8 massas solares na sequência principal, incluem objetos com idades e populações diferentes, o que as torna especialmente interessantes ao estudo da variação temporal dos gradientes. Neste trabalho, apresentamos resultados recentes sobre a variação temporal do gradiente de O/H a partir de uma amostra contendo cerca de 200 nebulosas, para as quais obtivemos distâncias por métodos estatísticos. As abundâncias foram parcialmente obtidas a partir de observações em Itajubá e La Silla, sendo as restantes obtidas da literatura. Os resultados mostram, pela primeira vez, evidências de um achatamento do gradiente de O/H, de -0.11 dex/kpc para -0.06 dex/kpc nos últimos 9 giga-anos ou de -0.08 dex/kpc para -0.06 dex/kpc nos últimos 5 giga-anos. Esses valores apresentam um bom acordo com alguns modelos recentes para a evolução química da Galáxia. (FAPESP/CNPq)

  12. Gastric parietal cell antigens of 60-90, 92, and 100-120 kDa associated with autoimmune gastritis and pernicious anemia. Role of N-glycans in the structure and antigenicity of the 60-90-kDa component.

    PubMed

    Goldkorn, I; Gleeson, P A; Toh, B H

    1989-11-01

    Thirty-four human sera containing parietal cell autoantibodies (PCA) specifically immunoprecipitated two antigens, with apparent molecular masses of 60-90 kDa and 100-120 kDa under nonreducing conditions and 60-90 kDa and 120-150 kDa under reducing conditions, from porcine gastric membrane extracts. A third antigen of 92 kDa was only observed in immunoprecipitates analyzed under reducing conditions. By immunoblotting, 24 of the 34 PCA-positive sera reacted with only the 60-90-kDa antigen, three reacted with a broad 60-120-kDa smear, one reacted only with a 92-kDa antigen and six did not react. Reactivity with the 60-90-kDa antigen was observed with gastric membranes from dog, pig, rat, and rabbit. Twenty PCA-negative sera did not react with these components by immunoprecipitation or immunoblotting. PCA reactivity with the 60-90-kDa antigen was abolished when the gastric membranes were (a) digested with Pronase, (b) reduced with 100 mM dithiothreitol, (c) treated with sodium periodate, or (d) digested with N-glycanase. The 60-90-kDa and 100-120-kDa components were insensitive to neuraminidase treatment. N-glycanase digestion of 125I-labeled antigens purified by immunoprecipitation and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis collapsed the 60-90-kDa antigen to a sharp 34-kDa band; the 100-120-kDa component was unaffected. These observations suggest that (i) parietal cell antigens comprise three components of 60-90, 92, and 100-120 kDa; (ii) the epitopes differ in conformational sensitivity; (iii) the 60-90-kDa antigen is a conserved molecule comprising a 34-kDa core protein extensively glycosylated with N-linked oligosaccharides; (iv) sialic acid residues are not present in the 60-90- and 100-120-kDa molecules, and (v) the carbohydrate and protein moieties of the 60-90-kDa molecule are required for antibody binding.

  13. [Adaptation and psychometric proprieties study for the Portuguese version of the Adolescent Coping Scale - Escala de Coping para Adolescentes].

    PubMed

    Guerreiro, Diogo Frasquilho; Cruz, Diana; Figueira, Maria Luísa; Sampaio, Daniel

    2014-01-01

    variáveis culturais, geográficas e sócioeconómicas.Conclusão: O presente estudo representa uma parte importante do protocolo de validação Portuguesa da Adolescent Coping Scale, nomeadamente a sua adaptação linguística, estudo da consistência interna e da estrutura fatorial.

  14. Analyzing the relation between the microbial diversity of DaQu and the turbidity spoilage of traditional Chinese vinegar.

    PubMed

    Li, Pan; Li, Sha; Cheng, Lili; Luo, Lixin

    2014-07-01

    Vinegar is a traditional fermented condiment, and the microbial diversity of DaQu makes the quality of vinegar products. Recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. In this study, the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar was analyzed by plating technique, PCR-denaturing gradient gel electrophoresis (DGGE), and high-performance liquid chromatography (HPLC). The 16S rRNA sequencing and DGGE analysis indicated that Bacillus (Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus thuringiensis) and Lactobacillus (including Lactobacillus acidipiscis and Lactobacillus pobuzihii) species were the dominant contaminants in vinegar products. Meanwhile, DGGE analysis showed that the dominant bacteria in DaQu belonged to genera Bacillus, Lactobacillus, Pseudomonas, Weissella, Saccharopolyspora, Enterobacter, and Pantoea. However, only two yeast species (Pichia kudriavzevii and Saccharomycopsis fibuligera) and seven mold species including Aspergillus oryzae, Aspergillus niger, Aspergillus candidus, Rhizopus microspores, Eurotium herbariorum, Absidia corymbifera, and Eupenicillium javanicum were detected in the DaQu. The population level of fungi was below 5 log CFU/g in DaQu. The chemical and physical properties of vinegar and sediments were also determined. On the basis of a combined microbial diversity-chemical analysis, we demonstrated that turbidity spoilage of vinegar was a result of cooperation among the low population level and abundance of fungal species in DaQu, the suitable climate conditions, and the contaminants in vinegar. This is the first report to analyze the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar.

  15. Analyzing the relation between the microbial diversity of DaQu and the turbidity spoilage of traditional Chinese vinegar.

    PubMed

    Li, Pan; Li, Sha; Cheng, Lili; Luo, Lixin

    2014-07-01

    Vinegar is a traditional fermented condiment, and the microbial diversity of DaQu makes the quality of vinegar products. Recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. In this study, the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar was analyzed by plating technique, PCR-denaturing gradient gel electrophoresis (DGGE), and high-performance liquid chromatography (HPLC). The 16S rRNA sequencing and DGGE analysis indicated that Bacillus (Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus thuringiensis) and Lactobacillus (including Lactobacillus acidipiscis and Lactobacillus pobuzihii) species were the dominant contaminants in vinegar products. Meanwhile, DGGE analysis showed that the dominant bacteria in DaQu belonged to genera Bacillus, Lactobacillus, Pseudomonas, Weissella, Saccharopolyspora, Enterobacter, and Pantoea. However, only two yeast species (Pichia kudriavzevii and Saccharomycopsis fibuligera) and seven mold species including Aspergillus oryzae, Aspergillus niger, Aspergillus candidus, Rhizopus microspores, Eurotium herbariorum, Absidia corymbifera, and Eupenicillium javanicum were detected in the DaQu. The population level of fungi was below 5 log CFU/g in DaQu. The chemical and physical properties of vinegar and sediments were also determined. On the basis of a combined microbial diversity-chemical analysis, we demonstrated that turbidity spoilage of vinegar was a result of cooperation among the low population level and abundance of fungal species in DaQu, the suitable climate conditions, and the contaminants in vinegar. This is the first report to analyze the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar. PMID:24691870

  16. Synergetic effects of DA-6/GA₃ with EDTA on plant growth, extraction and detoxification of Cd by Lolium perenne.

    PubMed

    He, Shanying; Wu, Qiuling; He, Zhenli

    2014-12-01

    Research is needed to improve efficiency of phytoextraction of heavy metals from contaminated soils. A pot experiment was carried out to study the effects of plant growth regulators (PGRs) (diethyl aminoethyl hexanoate (C18H33NO8, DA-6) and gibberellic acid 3 (C19H22O6, GA3)) and/or EDTA on Cd extraction, subcellular distribution and chemical forms in Lolium perenne. The addition of EDTA or PGRs significantly enhanced Cd extraction efficiency (P<0.05), with the decreasing order of: 1 μM DA-6>10 μM DA-6>10 μM GA3>2.5 mmol kg(-1) EDTA>other treatments of PGR alone. PGRs+EDTA resulted in a further increase in Cd extraction efficiency, with EDTA+1 μM DA-6 being the most efficient. At the subcellular level, about 44-57% of Cd was soluble fraction, 18-44% in cell walls, and 12-25% in cellular organelles fraction. Chemical speciation analysis showed that 40-54% of Cd was NaCl extractable, 7-23% HAc extractable, followed by other fractions. EDTA increased the proportions of Cd in soluble and cellular organelles fraction, as well as the metal migration in shoot; therefore, the toxicity to plant increased and plant growth was inhibited. Conversely, PGRs fixed more Cd in cell walls and reduced Cd migration in shoot; thus, metal toxicity was reduced. In addition, PGRs promoted plant biomass growth significantly (P<0.05), with 1 μM DA-6 being the most effective. A combination of DA-6/GA3 with EDTA can alleviate the adverse effect of EDTA on plant growth, and the treatment of EDTA+1 μM DA-6 appears to be optimal for improving the remediation efficiency of L. perenne for Cd contaminated soil. PMID:24999226

  17. Synergetic effects of DA-6/GA₃ with EDTA on plant growth, extraction and detoxification of Cd by Lolium perenne.

    PubMed

    He, Shanying; Wu, Qiuling; He, Zhenli

    2014-12-01

    Research is needed to improve efficiency of phytoextraction of heavy metals from contaminated soils. A pot experiment was carried out to study the effects of plant growth regulators (PGRs) (diethyl aminoethyl hexanoate (C18H33NO8, DA-6) and gibberellic acid 3 (C19H22O6, GA3)) and/or EDTA on Cd extraction, subcellular distribution and chemical forms in Lolium perenne. The addition of EDTA or PGRs significantly enhanced Cd extraction efficiency (P<0.05), with the decreasing order of: 1 μM DA-6>10 μM DA-6>10 μM GA3>2.5 mmol kg(-1) EDTA>other treatments of PGR alone. PGRs+EDTA resulted in a further increase in Cd extraction efficiency, with EDTA+1 μM DA-6 being the most efficient. At the subcellular level, about 44-57% of Cd was soluble fraction, 18-44% in cell walls, and 12-25% in cellular organelles fraction. Chemical speciation analysis showed that 40-54% of Cd was NaCl extractable, 7-23% HAc extractable, followed by other fractions. EDTA increased the proportions of Cd in soluble and cellular organelles fraction, as well as the metal migration in shoot; therefore, the toxicity to plant increased and plant growth was inhibited. Conversely, PGRs fixed more Cd in cell walls and reduced Cd migration in shoot; thus, metal toxicity was reduced. In addition, PGRs promoted plant biomass growth significantly (P<0.05), with 1 μM DA-6 being the most effective. A combination of DA-6/GA3 with EDTA can alleviate the adverse effect of EDTA on plant growth, and the treatment of EDTA+1 μM DA-6 appears to be optimal for improving the remediation efficiency of L. perenne for Cd contaminated soil.

  18. The prolactin gene is expressed in the hypothalamic-neurohypophyseal system and the protein is processed into a 14-kDa fragment with activity like 16-kDa prolactin.

    PubMed Central

    Clapp, C; Torner, L; Gutiérrez-Ospina, G; Alcántara, E; López-Gómez, F J; Nagano, M; Kelly, P A; Mejía, S; Morales, M A; Martínez de la Escalera, G

    1994-01-01

    The 23-kDa form of prolactin (PRL) has been proposed to function as both a mature hormone and a prohormone precursor for different uniquely bioactive forms of the molecule. We have shown that the 16-kDa N-terminal fragment of PRL (16K PRL) inhibits angiogenesis via a specific receptor. In addition, 16K PRL stimulates natriuresis and diuresis in the rat, and kidney membranes contain high-affinity specific binding sites for this PRL fragment. 16K PRL can be derived from an enzymatically cleaved form of PRL (cleaved PRL). With the use of a specific 16K PRL antiserum, we have localized a 14-kDa immunoreactive protein in the paraventricular and supraoptic nuclei of the hypothalamus and in the neurohypophysis. Reverse transcription-polymerase chain reaction of RNA from isolated paraventricular nuclei showed the expression of the full-length PRL mRNA. The neurohypophysis was found to contain the enzymes that produce cleaved PRL, small amounts of PRL, and cleaved PRL. Medium conditioned by neurohypophyseal cultures, enriched with the 14-kDa immunoreactive protein, has antiangiogenic effects that are blocked by the 16K PRL antiserum. These results are consistent with the expression of PRL in the hypothalamic-neurohypophyseal system, and the preferential processing of the protein into a 14-kDa fragment with biological and immunological properties of 16K PRL. Images PMID:7937959

  19. Análise da medição do raio solar em ultravioleta

    NASA Astrophysics Data System (ADS)

    Saraiva, A. C. V.; Giménez de Castro, C. G.; Costa, J. E. R.; Selhorst, C. L.; Simões, P. J. A.

    2003-08-01

    A medição acurada do raio solar em qualquer banda do espectro eletromagnético é de relevância na formulação e calibração de modelos da estrutura e atmosfera solar. Esses modelos atribuem emissão do contínuo do Sol calmo em microondas à mesma região da linha Ha do Hell. Apresentamos a medição do raio solar em UV com imagens do EIT (Extreme Ultraviolet Image Telescope) entre 1996 e 2002, no comprimento de onda 30,9 nm (Ha do Hell), que se forma na região de transição/cromosfera solar. A técnica utilizada para o cálculo do raio UV foi baseada na transformada Wavelet B3spline. Fizemos um banco de dados com 1 imagem por dia durante o período citado. Obtivemos como resultado o raio médio da ordem de 975.61" e uma diminuição do mesmo para o período citado variando em média -0,45" /ano. Comparamos estes dados com os valores obtidos pelo ROI (Radio Observatório de Itapetinga) em 22/48 GHz e Nobeyama Radio Heliograph em 17 GHz mostrando que os raios médios são muito próximos o que indica que a região de formação nessas freqüências é a mesma conforme os modelos. Comparamos os resultados também com outros índices de atividade solar.

  20. Structural requirements to obtain highly potent and selective 18 kDa Translocator Protein (TSPO) Ligands.

    PubMed

    Taliani, Sabrina; Pugliesi, Isabella; Da Settimo, Federico

    2011-01-01

    The (18 kDa) Translocator Protein (TSPO), was initially identified in 1977 as peripheral binding site for the benzodiazepine diazepam and named "Peripheral-type benzodiazepine receptor (PBR)". It is an evolutionarily well-conserved protein particularly located at the outer/inner mitochondrial membrane contact sites, in closely association with the 32 kDa voltage-dependent anion channel (VDAC) and the 30 kDa adenine nucleotide translocase (ANT), thus forming the mitochondrial permeability transition pore (MPTP). TSPO is ubiquitary expressed in peripheral tissues (steroid producing tissues, liver, heart, kidney, lung, immune system) and in lower levels in the central nervous system, where it is mainly located in glial cells, and in neurons. TSPO is involved in a variety of biological processes such as cholesterol transport, steroidogenesis, calcium homeostasis, lipid metabolism, mitochondrial oxidation, cell growth and differentiation, apoptosis induction, and regulation of immune functions. In the last decade, many studies have reported that TSPO basal expression is altered in a number of human pathologies, such as cancer and neurodegenerative disorders (Huntington's and Alzheimer's diseases), as well as in various forms of brain injury and inflammation and anxiety. Consequently, TSPO has not only been suggested as a promising drug target for a number of therapeutic applications (anticonvulsant, anxiolytic, immunomodulating, etc.), but also as valid diagnostic marker for related-disease state and progression, prompting the development of specific labelled ligands as powerful tools for imaging techniques. A number of structurally different classes of ligands have been reported, showing high affinity and selectivity towards TSPO. Indeed, most of these ligands have been designed starting from selective CBR ligands which were structurally modified in order to shift their affinity towards TSPO. Extensive structure-activity relationship studies were performed allowing to

  1. Abnormality of epiphyseal plate induced by selenium deficiency diet in two generation DA rats.

    PubMed

    Min, Zixin; Zhao, Wenxiang; Zhong, Nannan; Guo, Yuanxu; Sun, Mengyao; Wang, Quancheng; Zhang, Rui; Yan, Jidong; Tian, Lifang; Zhang, Fujun; Han, Yan; Ning, Qilan; Meng, Liesu; Sun, Jian; Lu, Shemin

    2015-08-01

    This study aimed to observe the effects of Se deficiency on epiphyseal plates of two generation DA rats fed with artificial total synthetic low Se diet. All F0 and F1 DA rats were fed with synthetic low Se diet (SeD group) and low Se diet supplied with Se (SeS group). The levels of selenium and enzyme activities of GPx were detected in plasma of the rats. General growth of bone and articular cartilage was measured macroscopically and microscopically. The epiphyseal plate of femur heads or tibia were obtained to histological and immunohistochemical examinations. The cartilage from left knee joints and femur heads was used to detect the gene expression of collagens, ADAMTSs and several selenoproteins by RT-qPCR. Two generation SeD rats showed Se insufficiency status. The thicknesses of the femur and tibial epiphyseal plates in both F0 and F1 SeD rats were significantly less than that of SeS rats. In F1 generation, SeD rats showed much fewer proliferative chondrocyte layers than SeS ones. Importantly, two generation SeD rats both showed significantly more serious pathological changes of epiphyseal plates. In two generation rats, gene expressions of COL II, GPx1 and GPx4 were significantly down-regulated in SeD rats than SeS ones; meanwhile ADAMTS-4 showed an up-regulated expression in cartilage. Dietary Se deficiency can apparently cause epiphyseal plate lesion and decrease cartilage type II collagen production and GPx1 activity in two generation DA rats fed with the artificial total synthesis low Se diet.

  2. [The Vitruvian Man: an anatomical drawing for proportions by Leonardo Da Vinci].

    PubMed

    Le Floch-Prigent, P

    2008-12-01

    The aim of the study was to find out and to analyse the text by Vitruvius which inspired the famous drawing by Leonardo da Vinci (circa 1490) kept in the Galleria dell'Accademia, in Venezia, Italy: the man inscribed in one circle and in one square. The book "de Architectura" by Vitruvius Marcus Pollio was printed several times since the Renaissance when both the roman architecture of antiquity and this text became very popular. From a French translation by Claude Perrault in 1864, it became easy to find a French translation with the original text in Latin (Paris, 2003, Les Belles Lettres, French text by Pierre Gros). The drawing by Leonardo da Vinci illustrates with great accuracy and fidelity the quotation of Vitruvius (with the exception of two of the 12 main relationships). The genius of Leonardo da Vinci was to keep only one trunk, head and neck for two pairs of limbs: scapular and pelvic; to make the circle tangent to the lower edge of the square; to adjust a few features of the quotation for the equilibrium of the whole figure; and of course to bring his incredible skill as a drawer (one of the best of his century). The drawing was made on a sheet of paper 344x245mm, in black ink which became dark brown with time; several lines complete the figure above and below; a short caption and a horizontal scale appear just under the drawing. The celebrity of the drawing, a symbol of the Renaissance, of the equilibrium of man and mankind, of the universality of the artists and intellectuals of the time (Humanism) made it iconic and it has been constantly reproduced and adapted especially for advertisement and logos, not only in the medical field.

  3. Identification and characterization of a novel 34 kDa merozoite protein in Babesia orientalis.

    PubMed

    He, Lan; Fan, Lizhe; Liu, Qin; Hu, Jinfang; Miao, Xiaoyan; Huang, Yuan; He, Pei; He, Junwei; Yu, Long; Khan, Muhammad Kasib; Zhou, Yanqin; Shen, Bang; Zhao, Junlong

    2015-09-15

    A novel Babesia orientalis 34 kDa protein (designated BoP34) was obtained by immunoscreening of a cDNA expression library using B. orientalis infected water buffalo serum. The complete nucleotide sequence of the BoP34 was 1088 bp, which contained one open reading frame (ORF), two untranslated regions (UTRs) and a poly (A) tail. The length of ORF was 933 bp, encoding a polypeptide of 310 aa with a predicted size of 34 kDa. BLAST analysis showed that the nucleotide sequence of BoP34 had 71% similarity with that of the Babesia bovis gene XM_001611335, which encodes a nuclear movement family protein. This suggested that BoP34 is a homologous of the movement family protein. Structural analysis of the BoP34 protein indicated a CS domain which may interact with the ATPase domain of the heat shock protein 90. A truncated version of BoP34 was cloned into the expression vector pET-32a and subsequently expressed and purified from the Escherichia coli Rosetta™ (DE3) pLysS stain as a Trx-fusion (designated rBoP34-T). Antibodies in the serum of a B. orientalis-infected water buffalo were able to recognize this protein in immune-bloting analysis. Rabbit antibodies raised against rBoP34-T could detecte native BoP34 (34 kDa) in B. orientalis-infected water buffalo erythrocytes. These results suggested that BoP34 might be a good diagnostic antigen for the specific detection of anti-B. orientalis antibody in water buffalo. Further research is required to explore the biological function and diagnostic potential of this molecule.

  4. 11 CFR 111.15 - Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 11 Federal Elections 1 2012-01-01 2012-01-01 false Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). 111.15 Section 111.15 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.15 Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). (a) Any person...

  5. 11 CFR 111.15 - Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 11 Federal Elections 1 2013-01-01 2012-01-01 true Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). 111.15 Section 111.15 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111.15 Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). (a) Any person...

  6. Associated Da Vinci and magellan robotic systems for successful treatment of nutcracker syndrome.

    PubMed

    Thaveau, Fabien; Nicolini, Philippe; Lucereau, Benoit; Georg, Yannick; Lejay, Anne; Chakfe, Nabil

    2015-01-01

    Here, we report the case of a 26-year-old woman suffering from nutcracker syndrome with concurrent disabling pelvic congestion syndrome. She was given the minimally invasive treatment of left renal vein transposition with the Da Vinci(®) robotic system (Intuitive Surgical, Sunnyvale, CA), followed the next day by a gonadal vein and pelvic varicose embolization using a robotic intraluminal navigation with the Magellan™ robotic system (Hansen Medical, Mountain View, CA). The procedure was uneventful, and the patient had good results at 6 months of follow-up, including a patent left renal vein and complete relief of symptoms.

  7. Microfossils in micrites from Serra da Bodoquena (MS), Brazil: taxonomy and paleoenvironmental implications.

    PubMed

    Utida, Giselle; Petri, Setembrino; Oliveira, Emiliano C; Boggiani, Paulo C

    2012-06-01

    Microfossils present in Quaternary micrites from Serra da Bodoquena, Mato Grosso do Sul State, Brazil, are here described for the first time. The studied taxa are: a) ostracods: Candona sp., Candonopsis sp., Cyclocypris sp., Cypria sp., Cypridopsis sp., Notodromas sp., Ilyocypris sp., Cyprideis sp., Wolburgiopsis cf. chinamuertensis (Musacchio 1970), Darwinula sp. and 5 morphotypes; b) microgastropod Acrobis sp., and c) Characeae remains and gyrogonites Chara sp.. The presence of these microfossils suggests clear-water shallow lacustrine paleoenvironments and the presence of aquatic vegetation. Similarities between microfossils and the living taxa suggest possible Holocene ages for these deposits, which is in accordance with previous C(14) dates.

  8. Molluscan mega-hemocyanin: an ancient oxygen carrier tuned by a ~550 kDa polypeptide

    PubMed Central

    2010-01-01

    Background The allosteric respiratory protein hemocyanin occurs in gastropods as tubular di-, tri- and multimers of a 35 × 18 nm, ring-like decamer with a collar complex at one opening. The decamer comprises five subunit dimers. The subunit, a 400 kDa polypeptide, is a concatenation of eight paralogous functional units. Their exact topology within the quaternary structure has recently been solved by 3D electron microscopy, providing a molecular model of an entire didecamer (two conjoined decamers). Here we study keyhole limpet hemocyanin (KLH2) tridecamers to unravel the exact association mode of the third decamer. Moreover, we introduce and describe a more complex type of hemocyanin tridecamer discovered in fresh/brackish-water cerithioid snails (Leptoxis, Melanoides, Terebralia). Results The "typical" KLH2 tridecamer is partially hollow, whereas the cerithioid tridecamer is almost completely filled with material; it was therefore termed "mega-hemocyanin". In both types, the staggering angle between adjoining decamers is 36°. The cerithioid tridecamer comprises two typical decamers based on the canonical 400 kDa subunit, flanking a central "mega-decamer" composed of ten unique ~550 kDa subunits. The additional ~150 kDa per subunit substantially enlarge the internal collar complex. Preliminary oxygen binding measurements indicate a moderate hemocyanin oxygen affinity in Leptoxis (p50 ~9 mmHg), and a very high affinity in Melanoides (~3 mmHg) and Terebralia (~2 mmHg). Species-specific and individual variation in the proportions of the two subunit types was also observed, leading to differences in the oligomeric states found in the hemolymph. Conclusions In cerithioid hemocyanin tridecamers ("mega-hemocyanin") the collar complex of the central decamer is substantially enlarged and modified. The preliminary O2 binding curves indicate that there are species-specific functional differences in the cerithioid mega-hemocyanins which might reflect different physiological

  9. The morphostructure of the South Atlantic Ridge in the Tristan da Cunha hot spot area

    NASA Astrophysics Data System (ADS)

    Ilyin, A. V.

    2016-06-01

    Investigation of the rift zone and flanks of the South Atlantic midoceanic ridge in the Tristan da Cunha hot spot area revealed that their morphostructure is atypical of slow spreading ridges. Feeding the spreading center with magmatic material, the hot spot changes the morphology of the axial zone and transform faults. It itself forms a relief ensemble that differs from the rift one as well. The continuous migration of the spreading center in the westerly direction stimulates the formation of a spacious volcanic highland, which is responsible for the regional asymmetry of ridge flanks in its extended segments (hundreds kilometers long).

  10. Surgical treatment of parietal defects with “da Vinci” surgical robot

    PubMed Central

    Vasilescu, D; Paun, S

    2012-01-01

    The robotic surgery has come through the development of telemedicine and minimally invasive surgery concepts, being developed in the military medicine by NASA during the years 1970-1980. The purpose of this paper is to briefly present our experience in the new field of the robotic surgery, by analyzing the results obtained over a lot of 20 patients operated with the “da Vinci” robot within the last 5 years in the Clinical Emergency Hospital Bucharest for various abdominal defects PMID:22802899

  11. The first Brazilian neuropsychiatrist, José Martins da Cruz Jobim, tuberculous meningitis and mental disease.

    PubMed

    Gomes, Marleide da Mota; Engelhardt, Eliasz; Chimelli, Leila

    2013-03-01

    The intersection between infectious diseases, poverty and mental disease was an important subject to the Brazilian Neuropsychiatry in the early 19th century. José Martins da Cruz Jobim (1802-1878) was engaged in a hygienist approach based on symptomatological and anatomopathological studies. He wrote "Insânia loquaz" (Loquacious insanity), 1831, the first written text about mental illness in Brazil, founded on clinical and pathological data, compatible with tuberculous meningitis. Thus, Jobim deserves the title of the first neuropsychiatrist in Brazil. The authors critically studied the clinical history and the autopsy findings of his cases, and the main health policies at the time.

  12. The magmatic source of the Tristan da Cunha hotspot: Implication from electrical conductivity

    NASA Astrophysics Data System (ADS)

    Chen, Jin; Baba, Kiyoshi; Jegen, Marion; Utada, Hisashi; Geissler, Wolfram; Jokat, Wilfried

    2016-04-01

    Tristan da Cunha Island is one of the classical hot spots in the Atlantic Ocean, situated at the western end of the aseismic Walvis Ridge which forms a connection to the Cretaceous Etendeka flood basalt province in northwestern Namibia. The discussion about its source (in shallow asthenosphere or deeper mantle) have not reached consensus yet because of lack of the geophysical observations in the area. A marine magnetotelluric (MT) experiment was conducted together with seismological observations in the area in 2012-2013 through a German-Japanese collaboration with the goal to constrain the physical state of the mantle beneath the area. A total of 26 MT seafloor stations were deployed around the Tristan da Cunha Islands and available data were retrieved and processed from 24 stations. We applied iterative topographic effect correction and one-dimensional (1-D) conductivity structure inversion to the data. Then, three-dimensional (3-D) inversion analysis incorporating the topographic effect was carried out, using the 1-D model as the initial model. The local small-scale topography and the far continental coast effects are incorporated as the distortion term in the 3-D inversion. The preliminary result of our analysis shows no evidence of a significant conductive anomaly arising from the mantle transition zone, suggesting that the current magmatic source (major place of melting) of the hotspot activity is in the shallow upper mantle. This is in contrast to results from geochemical analysis, in which samples along the Tristan track exhibit an ocean-island-basalt-type incompatible element pattern pointing to a deep mantle source of the melt. Our findings therefore might indicate that the deep mantle up-welling underneath Tristan da Cunha Islands may be almost dead. A conductive anomaly at approx. 100 km depth in our derived conductivity model to the southwest of Tristan da Cunha Islands suggests an interaction between the mid-ocean ridge and/or up-welling further south

  13. [The anatomy of a reduced skull model--visualisation of Leonardo da Vinci's anthropology].

    PubMed

    Ahner, E

    2008-04-01

    The article focuses on a rare example of a miniature skull of unknown origin. The profoundness of the anatomical details, conjoint with outstanding virtuosity, reminds of Leonardo da Vinci's anatomical skull studies and asks for additional interpretation beside the emblematic "memento mori"-character. Following the miscellaneous topics of his skull studies an anatomical-anthropological interpretation is proposed. For such a project the mergence of anthropology, history of medicine and history of art was mandatory. Concerning some discrepancies within the anatomical realism, the depiction of a pathology is discussed and beyond the visualisation of a historic concept of brain function.

  14. Early experience with telemanipulative robot-assisted laparoscopic cholecystectomy using da Vinci.

    PubMed

    Kim, Victor B; Chapman, William H H; Albrecht, Robert J; Bailey, B Marcus; Young, James A; Nifong, L Wiley; Chitwood, W Randolph

    2002-02-01

    In the past decade, robot-assisted surgery has become increasingly used to assist in minimally invasive surgical procedures. In this article we review the evolution of robotic devices, from the first use of an industrial robot for stereotactic biopsies to pioneering work with robots used for hip and prostate surgery, to the development of robotic guidance systems that enabled solo endoscopic surgery, to telemanipulative surgery with master-servant computer-enhanced robotic devices. In addition, we review our early experience with da Vinci Robotic Surgical Systems (Intuitive Surgical, Inc., Mountain View, CA, U.S.A.), which we used to perform robot-assisted laparoscopic cholecystectomies.

  15. Middle Paleolithic human remains from the Gruta da Oliveira (Torres Novas), Portugal.

    PubMed

    Trinkaus, Erik; Maki, Julia; Zilhão, João

    2007-10-01

    Ongoing excavations in the Middle Paleolithic levels at the Gruta da Oliveira, Portugal have yielded four fragmentary human remains, a manual phalanx and an ulna from levels 9 and 10, and a humerus and a tibia from levels 18 and 19. The first two remains date to approximately 39 ka 14C BP ( approximately 43.5 ka cal BP), whereas the latter two derive from earlier in oxygen isotope stage 3. The preserved portions of the phalanx, humerus, and tibia align them morphologically with the Neandertals. In addition, the Oliveira 4 tibial diaphysis shows evidence of carnivore (probably canid) gnawing.

  16. Is the DA white dwarf 1910 + 047 a soft X-ray source?

    NASA Technical Reports Server (NTRS)

    Vennes, Stephane

    1990-01-01

    The possibility that the recently discovered DA white dwarf WD 1910 + 047 might be an Einstein soft X-ray source is studied, applying a complete model atmosphere analyis to both the optical and the soft X-ray data. It is found that the X-ray source in question is at least one order of magnitude too strong to be compatible with the estimated atmospheric parameters of the possible optical counterpart. The spatial coincidence between the two, however, remains extremely intriguing and has left us with the dilemma of accepting the relatively improbable coincidence of two unrelated objects or rejoicing over the discovery of a highly unusual one.

  17. [Revista Brasileira de História da Medicina, a pioneer in medical historiography].

    PubMed

    Mendonça, Amanda; Nicolini, Gabriel Baptista

    2007-01-01

    I(It? This?) presents the general characteristics of the Revista Brasileira de História da Medicina, which contributed for the emergence of the field of medicine history in Brazil by inciting bibliographic production on the subject. The purpose of our analysis is to foster research on the history of medicine and of the sciences. From the description of the editorial aspects of the journal one can have an idea of how the research field was organized between 1940 and 1960. The periodical also allows for an overview of the exchange occurring between Brazilian and foreign historian physicians at that time.

  18. Complexes of 3.6 kDa maltodextrin with some metals.

    PubMed

    Tomasik, Piotr; Anderegg, James W; Schilling, Christopher H

    2004-06-30

    Preparation of magnesium, lanthanum, and bismuth(III) complexes of 3.6 kDa maltodextrin and some properties of the resulting materials are presented. The metal derivatives contain metals bound to the oxygen atoms of the hydroxyl groups of maltodextrin. Additionally, the metal atoms are coordinated to the hydroxyl groups of the D-glucose units of the macroligand. Such coordination stabilized the metal - oxygen bond against hydrolysis, even in boiling water. The presence of magnesium and lanthanum atoms increased the thermal stability of maltodextrin, whereas bismuth atoms decreased it.

  19. The strainrange partitioning behavior of an advanced gas turbine disk alloy, AF2-1DA

    NASA Technical Reports Server (NTRS)

    Halford, G. R.; Nachtigall, A. J.

    1979-01-01

    The low-cycle, creep-fatigue characteristics of the advanced gas turbine disk alloy, AF2-1DA have been determined at 1400 F and are presented in terms of the method of strainrange partitioning (SRP). The mean stresses which develop in the PC and CP type SRP cycles at the lowest inelastic strainrange were observed to influence the cyclic lives to a greater extent than the creep effects and hence interfered with a conventional interpretation of the results by SRP. A procedure is proposed for dealing with the mean stress effects on life which is compatible with SRP.

  20. OCT structural examination of Madonna dei Fusi by Leonardo da Vinci

    NASA Astrophysics Data System (ADS)

    Targowski, Piotr; Iwanicka, Magdalena; Sylwestrzak, Marcin; Kaszewska, Ewa A.; Frosinini, Cecilia

    2013-05-01

    Madonna dei Fusi (`Madonna of the Yarnwider') is a spectacular example of Italian Renaissance painting, attributed to Leonardo da Vinci. The aim of this study is to give an account of past restoration procedures. The evidence of a former retouching campaign will be presented with cross-sectional images obtained non-invasively with Optical Coherence Tomography (OCT). Specifically, the locations of overpaintings/retouchings with respect to the original paint layer and secondary varnishes will be given. Additionally, the evidence of a former transfer of the pictorial layer to the new canvas support by detecting the presence of its structure incised into paint layer will be shown.

  1. Quadrupole Ion Mass Spectrometer for Masses of 2 to 50 Da

    NASA Technical Reports Server (NTRS)

    Helms, William; Griffin, Timothy P.; Ottens, Andrew; Harrison, Willard

    2005-01-01

    A customized quadrupole ion-trap mass spectrometer (QITMS) has been built to satisfy a need for a compact, rugged instrument for measuring small concentrations of hydrogen, helium, oxygen, and argon in a nitrogen atmosphere. This QITMS can also be used to perform quantitative analyses of other gases within its molecular-mass range, which is 2 to 50 daltons (Da). (More precisely, it can be used to perform quantitative analysis of gases that, when ionized, are characterized by m/Z ratios between 2 and 50, where m is the mass of an ion in daltons and Z is the number of fundamental electric charges on the ion.

  2. The discontinuity near 1600 A in the spectra of DA white dwarfs

    NASA Technical Reports Server (NTRS)

    Wegner, G.

    1984-01-01

    Ultraviolet spectroscopic observations of two relatively cool DA white dwarfs, L481 - 60 (= WD 1544 - 37) and BPM 1266 ( = WD 2105 - 82), with the International Ultraviolet Explorer (IUE) satellite show a strong drop in their spectral energy distributions below 1600 A. Published model atmospheres and thier visual spectra suggest that these two stars have effective temperatures in the vicinity of 9,000-10,000 K, and it is proposed that the 1600 A feature could be due to the 342(1S) 3s2(1S) photoionization edge of Mg I.

  3. Middle Paleolithic human remains from the Gruta da Oliveira (Torres Novas), Portugal.

    PubMed

    Trinkaus, Erik; Maki, Julia; Zilhão, João

    2007-10-01

    Ongoing excavations in the Middle Paleolithic levels at the Gruta da Oliveira, Portugal have yielded four fragmentary human remains, a manual phalanx and an ulna from levels 9 and 10, and a humerus and a tibia from levels 18 and 19. The first two remains date to approximately 39 ka 14C BP ( approximately 43.5 ka cal BP), whereas the latter two derive from earlier in oxygen isotope stage 3. The preserved portions of the phalanx, humerus, and tibia align them morphologically with the Neandertals. In addition, the Oliveira 4 tibial diaphysis shows evidence of carnivore (probably canid) gnawing. PMID:17632802

  4. [Science and nation: romanticism and natural history in the works of E. J. da Silva Maia].

    PubMed

    Kury, L

    1998-01-01

    The works of physician and naturalist Emílio Joaquim da Silva Maia (1808-59) can be viewed as a scientific project that discovers Brazil and its inhabitants. Maia's nationalism and his romantic view of nature formed the underpinnings of his scientific theories, especially his studies on zoological geography. He subordinated the issue of the biological specificity of different regions of the world to his era's debates on the construction of Brazil as an independent nation. In his interpretations of European natural history, Maia endeavored to understand Brazilian nature as a specific achievement of the Cosmos, in keeping with Alexander von Humboldt's approach. PMID:16676427

  5. Circulating non-22 kDa growth hormone isoforms after a repeated GHRH stimulus in normal subjects.

    PubMed

    Coya, R; Algorta, J; Boguszewski, C L; Vela, A; Carlsson, L M S; Aniel-Quiroga, A; Busturia, M A; Martul, P

    2005-04-01

    The aim of this study was to evaluate the proportion of non-22 kDa GH isoforms in relation to total GH concentration after a repeated GHRH stimulus in healthy subjects. We studied 25 normal volunteers (12 males and 13 females, mean age 13.1 years, range 6-35), who received two GHRH bolus (1.5 mug/kg body weight, i.v.) administered separately by an interval of 120 minutes. The proportion of non-22 kDa GH was determined by the 22 kDa GH exclusion assay (GHEA), which is based on immunomagnetic extraction of monomeric and dimeric 22 kDa GH from serum, and quantitation of non-22 kDa GH isoforms using a polyclonal GH assay. Samples were collected at baseline and at 15-30 min intervals up to 240 min for total GH concentration. Non-22 kDa GH isoforms were measured in samples where peak GH after GHRH was observed. Total GH peaked after the first GHRH bolus in all subjects (median 37.2 ng/ml; range: 10.4-94.6). According to GH response to the second GHRH stimulus, the study group was divided in "non-responders" (n=7; 28%), with GH peak levels lower than 10 ng/ml (median GH: 8.7 ng/ml; range 7.3-9.6) and "responders" (n=18; 72%), who showed a GH response greater than 10 ng/ml (median 17 ng/ml; range 10.1-47.0). The median proportion of non-22 kDa GH on the peak of GH secretion after the first GHRH administration was similar in both groups ("responders" median: 8.6%, range 7-10.9%; "non-responders" median: 8.7%, range 6.7-10.3%), independently of the type of response after the second GHRH. In contrast, the median proportion of non-22 kDa GH was greater at time of GH peak after the second GHRH bolus in the "non-responders" (median 11.4%; range 9.1-14.3%) in comparison with the "responders" (median 9.1%; range 6.7-11.9%; p=0.003). A significant negative correlation between the total GH secreted and the percentage of non-22 kDa isoforms was seen in the "non-responders" (p=0.003). These differences in GH response to repeated GHRH stimulation and in the pattern of GH isoforms at GH

  6. Rising from the Ashes: Mid-infrared Re-brightening of the Impostor SN 2010da in NGC 300

    NASA Astrophysics Data System (ADS)

    Lau, Ryan M.; Kasliwal, Mansi M.; Bond, Howard E.; Smith, Nathan; Fox, Ori D.; Carlon, Robert; Cody, Ann Marie; Contreras, Carlos; Dykhoff, Devin; Gehrz, Robert; Hsiao, Eric; Jencson, Jacob; Khan, Rubab; Masci, Frank; Monard, L. A. G.; Monson, Andrew J.; Morrell, Nidia; Phillips, Mark; Ressler, Michael E.

    2016-10-01

    We present multi-epoch mid-infrared (IR) photometry and the optical discovery observations of the “impostor” supernova (SN) 2010da in NGC 300 using new and archival Spitzer Space Telescope images and ground-based observatories. The mid-infrared counterpart of SN 2010da was detected as Spitzer Infrared Intensive Transient Survey (SPIRITS) 14bme in the SPIRITS, an ongoing systematic search for IR transients. Before erupting on 2010 May 24, the SN 2010da progenitor exhibited a constant mid-IR flux at 3.6 and only a slight ∼10% decrease at 4.5 μm between 2003 November and 2007 December. A sharp increase in the 3.6 μm flux followed by a rapid decrease measured ∼150 days before and ∼80 days after the initial outburst, respectively, reveal a mid-IR counterpart to the coincident optical and high luminosity X-ray outbursts. At late times, after the outburst (∼2000 days), the 3.6 and 4.5 μm emission increased to over a factor of two times the progenitor flux and is currently observed (as of 2016 Feb) to be fading, but still above the progenitor flux. We attribute the re-brightening mid-IR emission to continued dust production and increasing luminosity of the surviving system associated with SN 2010da. We analyze the evolution of the dust temperature (T d ∼ 700–1000 K), mass (M d ∼ 0.5–3.8 × 10‑7 M ⊙), luminosity (L IR ∼ 1.3–3.5 × 104 L ⊙), and the equilibrium temperature radius (R eq ∼ 6.4–12.2 au) in order to resolve the nature of SN 2010da. We address the leading interpretation of SN 2010da as an eruption from a luminous blue variable high-mass X-ray binary (HMXB) system. We propose that SN 2010da is instead a supergiant (sg)B[e]-HMXB based on similar luminosities and dust masses exhibited by two other known sgB[e]-HMXB systems. Additionally, the SN 2010da progenitor occupies a similar region on a mid-IR color–magnitude diagram (CMD) with known sgB[e] stars in the Large Magellanic Cloud. The lower limit estimated for the orbital

  7. Pharmacological profile of DA-6886, a novel 5-HT4 receptor agonist to accelerate colonic motor activity in mice.

    PubMed

    Lee, Min Jung; Cho, Kang Hun; Park, Hyun Min; Sung, Hyun Jung; Choi, Sunghak; Im, Weonbin

    2014-07-15

    DA-6886, the gastrointestinal prokinetic benzamide derivative is a novel 5-HT4 receptor agonist being developed for the treatment of constipation-predominant irritable bowel syndrome (IBS-C). The purpose of this study was to characterize in vitro and in vivo pharmacological profile of DA-6886. We used various receptor binding assay, cAMP accumulation assay, organ bath experiment and colonic transit assay in normal and chemically constipated mice. DA-6886 exhibited high affinity and selectivity to human 5-HT4 receptor splice variants, with mean pKi of 7.1, 7.5, 7.9 for the human 5-HT4a, 5-HT4b and 5-HT4d, respectively. By contrast, DA-6886 did not show significant affinity for several receptors including dopamine D2 receptor, other 5-HT receptors except for 5-HT2B receptor (pKi value of 6.2). The affinity for 5-HT4 receptor was translated into functional agonist activity in Cos-7 cells expressing 5-HT4 receptor splice variants. Furthermore, DA-6886 induced relaxation of the rat oesophagus preparation (pEC50 value of 7.4) in a 5-HT4 receptor antagonist-sensitive manner. The evaluation of DA-6886 in CHO cells expressing hERG channels revealed that it inhibited hERG channel current with an pIC50 value of 4.3, indicating that the compound was 1000-fold more selective for the 5-HT4 receptor over hERG channels. In the normal ICR mice, oral administration of DA-6886 (0.4 and 2mg/kg) resulted in marked stimulation of colonic transit. Furthermore, in the loperamide-induced constipation mouse model, 2mg/kg of DA-6886 significantly improved the delay of colonic transit, similar to 10mg/kg of tegaserod. Taken together, DA-6886 is a highly potent and selective 5-HT4 receptor agonist to accelerate colonic transit in mice, which might be therapeutic agent having a favorable safety profile in the treatment of gastrointestinal motor disorders such as IBS-C and chronic constipation.

  8. Da0324, an inhibitor of nuclear factor-κB activation, demonstrates selective antitumor activity on human gastric cancer cells

    PubMed Central

    Jin, Rong; Xia, Yiqun; Chen, Qiuxiang; Li, Wulan; Chen, Dahui; Ye, Hui; Zhao, Chengguang; Du, Xiaojing; Shi, Dengjian; Wu, Jianzhang; Liang, Guang

    2016-01-01

    Background The transcription factor nuclear factor-κB (NF-κB) is constitutively activated in a variety of human cancers, including gastric cancer. NF-κB inhibitors that selectively kill cancer cells are urgently needed for cancer treatment. Curcumin is a potent inhibitor of NF-κB activation. Unfortunately, the therapeutic potential of curcumin is limited by its relatively low potency and poor cellular bioavailability. In this study, we presented a novel NF-κB inhibitor named Da0324, a synthetic asymmetric mono-carbonyl analog of curcumin. The purpose of this study is to research the expression of NF-κB in gastric cancer and the antitumor activity and mechanism of Da0324 on human gastric cancer cells. Methods The expressions between gastric cancer tissues/cells and normal gastric tissues/cells of NF-κB were evaluated by Western blot. The inhibition viability of compounds on human gastric cancer cell lines SGC-7901, BGC-823, MGC-803, and normal gastric mucosa epithelial cell line GES-1 was assessed with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. Absorption spectrum method and high-performance liquid chromatography method detected the stability of the compound in vitro. The compound-induced changes of inducible NF-κB activation in the SGC-7901 and BGC-823 cells were examined by Western blot analysis and immunofluorescence methods. The antitumor activity of compound was performed by clonogenic assay, matrigel invasion assay, flow cytometric analysis, Western blot analysis, and Hoechst 33258 staining assay. Results High levels of p65 were found in gastric cancer tissues and cells. Da0324 displayed higher growth inhibition against several types of gastric cancer cell lines and showed relatively low toxicity to GES-1. Moreover, Da0324 was more stable than curcumin in vitro. Western blot analysis and immunofluorescence methods showed that Da0324 blocked NF-κB activation. In addition, Da0324 significantly inhibited tumor proliferation

  9. IrBurst Modeling and Performance Evaluation for Large Data Block Exchange over High-Speed IrDA Links

    NASA Astrophysics Data System (ADS)

    Alam, Mohammad Shah; Shawkat, Shamim Ara; Kitazumi, Gontaro; Matsumoto, Mitsuji

    IrBurst, recently proposed by IrDA, is a high speed information transmission protocol. In this paper, a mathematical model is developed which leads to derivation of the IrBurst throughput over the IrDA protocol stack. Based on this model, we compare the performance of IrBurst and existing OBEX protocol in order to investigate the suitability of IrBurst protocol for exchange of large data blocks over high-speed IrDA links. Furthermore, the model allows the evaluation of the impact of the link layer parameters, such as window size and frame length, and physical layer parameters, such as minimum turnaround time, on system through-put for high-speed IrDA links and in the presence of transmission errors. Consequently, an effective Automatic Repeat Request (ARQ) scheme is proposed at link layer to maximize the throughput efficiency for IrBurst protocol as well as for next generation high speed IrDA links. Simulation result indicates that employment of our proposed ARQ scheme results in significant improvement of IrBurst throughput efficiency at high bit error rates.

  10. New paleomagnetic constraints on the extrusion of Indochina: Late Cretaceous results from the Song Da terrane, northern Vietnam

    NASA Astrophysics Data System (ADS)

    Takemoto, Kazuhiro; Halim, Nadir; Otofuji, Yo-ichiro; Van Tri, Tran; Van De, Le; Hada, Shigeki

    2005-01-01

    Samples were collected for paleomagnetic investigations from the Upper Cretaceous Yen Chau Formation in the Song Da terrane (21.7°N, 103.9°E) bounded by the Ailao Shan-Red River fault system and Song Ma fault, in an attempt to examine extrusion tectonics of East Asia. Primary nature of the high-temperature (600-690 °C) component of magnetization from 13 sites is supported by a positive fold test. The tectonic corrected data provides the characteristic Late Cretaceous paleomagnetic direction for the Song Da terrane ( D=6.4°, I=32.0° with α95=8.5°, N=13 sites), corresponding to a paleopole lying at 82.9°N, 220.7°E ( A95=6.9°). Comparison with coeval paleomagnetic poles for the neighboring tectonic blocks indicates no latitudinal translation of the Son Da terrane with respect to the South China Block, whereas the Shan-Thai Block and the southern part of the Indochina Block revealed a southward displacement of 10.5±9.5° in latitude with respect to the Song Da terrane. We conclude that the southeastern segment of the Ailao Shan-Red River fault system to the east of the Dien Bien Phu fault is not a demarcation of the extruded Indochina Peninsula. The Indochina Block extruded probably along some faults between the Song Da terrane and the Khorat Plateau.

  11. Vŗkşăyurvĕda of parăśara--an ancient treatise on plant science.

    PubMed

    Prasad, G P; Neelima, G; Pratap, G P; Swamy, G K

    2006-01-01

    Vŗkşăyurvĕda of Parăśara is a great contribution to the Botany in ancient India. N.N. Sircar and Roma sarkar edited this text with English translation. Notes with comparative references of modern botany were added. This book can be placed in all probability in between 1st century B.C to 4th century A.D by its linguistic style. Many scientific branches of Botany including origin of life, ecology, distribution of forests, morphology, classification, nomenclature, histology and physiology were dealt in this ancient work. Though it is presumed that this book was written by Parăśara to teach Botany to preparatory to Ayurvĕda studies to ancient Ayurvĕda students, it is true to the Ayurvĕda personals and other disciplines related to Botany of present day as well. Aim of this article is to attract the attention of all scholars who are related to Ayurvĕda and Botany and to feel the depth of the knowledge of ancient Indian botany.

  12. DREADD Modulation of Transplanted DA Neurons Reveals a Novel Parkinsonian Dyskinesia Mechanism Mediated by the Serotonin 5-HT6 Receptor.

    PubMed

    Aldrin-Kirk, Patrick; Heuer, Andreas; Wang, Gang; Mattsson, Bengt; Lundblad, Martin; Parmar, Malin; Björklund, Tomas

    2016-06-01

    Transplantation of DA neurons is actively pursued as a restorative therapy in Parkinson's disease (PD). Pioneering clinical trials using transplants of fetal DA neuroblasts have given promising results, although a number of patients have developed graft-induced dyskinesias (GIDs), and the mechanism underlying this troublesome side effect is still unknown. Here we have used a new model where the activity of the transplanted DA neurons can be selectively modulated using a bimodal chemogenetic (DREADD) approach, allowing either enhancement or reduction of the therapeutic effect. We show that exclusive activation of a cAMP-linked (Gs-coupled) DREADD or serotonin 5-HT6 receptor, located on the grafted DA neurons, is sufficient to induce GIDs. These findings establish a mechanistic link between the 5-HT6 receptor, intracellular cAMP, and GIDs in transplanted PD patients. This effect is thought to be mediated through counteraction of the D2 autoreceptor feedback inhibition, resulting in a dysplastic DA release from the transplant.

  13. Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize.

    PubMed

    Liu, Hongjun; Shi, Junpeng; Sun, Chuanlong; Gong, Hao; Fan, Xingming; Qiu, Fazhan; Huang, Xuehui; Feng, Qi; Zheng, Xixi; Yuan, Ningning; Li, Changsheng; Zhang, Zhiyong; Deng, Yiting; Wang, Jiechen; Pan, Guangtang; Han, Bin; Lai, Jinsheng; Wu, Yongrui

    2016-05-01

    The maize opaque2 (o2) mutant has a high nutritional value but it develops a chalky endosperm that limits its practical use. Genetic selection for o2 modifiers can convert the normally chalky endosperm of the mutant into a hard, vitreous phenotype, yielding what is known as quality protein maize (QPM). Previous studies have shown that enhanced expression of 27-kDa γ-zein in QPM is essential for endosperm modification. Taking advantage of genome-wide association study analysis of a natural population, linkage mapping analysis of a recombinant inbred line population, and map-based cloning, we identified a quantitative trait locus (qγ27) affecting expression of 27-kDa γ-zein. qγ27 was mapped to the same region as the major o2 modifier (o2 modifier1) on chromosome 7 near the 27-kDa γ-zein locus. qγ27 resulted from a 15.26-kb duplication at the 27-kDa γ-zein locus, which increases the level of gene expression. This duplication occurred before maize domestication; however, the gene structure of qγ27 appears to be unstable and the DNA rearrangement frequently occurs at this locus. Because enhanced expression of 27-kDa γ-zein is critical for endosperm modification in QPM, qγ27 is expected to be under artificial selection. This discovery provides a useful molecular marker that can be used to accelerate QPM breeding.

  14. A estabilidade dos PAHS em função da energia da radiação interestelar nas faixas UV e raios-X

    NASA Astrophysics Data System (ADS)

    Pinotti, R.; Costa, R. K.; Boechat-Roberty, H. M.; Lago, A.; Souza, G. B.

    2003-08-01

    A nebulosa CRL 618, uma proto-nebulosa planetária cuja nuvem molecular espessa envolve uma estrela B0, contém uma grande quantidade de C2H2 e CH4. Estas moléculas são consideradas os tijolos da criação de grandes moléculas carbonadas como os Hidrocarbonetos Policíclicos Aromáticos (PAHs). Esta nebulosa, por estar exposta a intensos campos de UV e Raios-X, é uma região de fotodissociação molecular que propicia a formação de novas moléculas, confirmada pela presença de C4H2 e C6H6 (Benzeno), que é a unidade básica dos PAHs. Atribui-se a esta família de moléculas orgânicas duas propriedades fundamentais, a resistência para sobreviver ao campo de radiação UV interestelar e a geração das bandas de emissão não identificadas (UIR) observadas no infravermelho. No entanto, alguns autores questionam a resistência dos PAHs ao campo de radiação UV interestelar. Empregando a técnica de Espectrometria de Massas por Tempo de Vôo, no modo de coincidência fotoelétron-fotoíon, estudamos a ionização e fragmentação das seguintes moléculas: Benzeno, Benzeno deuterado, Naftaleno, Antraceno e Fenantreno. Utilizamos uma fonte de Hélio monocromática em 21,21 eV (584,5 Å) e a radiação Síncroton do Laboratório Nacional de Luz Síncroton (LNLS) em diferentes energias nas proximidades da borda do C 1s ( 290 eV). Comprovamos a estabilidade dos PAHs sob ação de UV (21,21 eV), onde eles apresentam um baixo nível de fotodissociação, produzindo fragmentos ionizados com rendimento total na ordem de 5 por cento em relação ao íon molecular pai. Entretanto, em altas energias, na faixa de Raios-X, a quebra destas moléculas torna-se mais intensa, com a produção de muitos fragmentos. Como uma das rotas de fragmentação do Naftaleno é [(C10H8) = > (C6H6+) + (C4H2) + (e-)], e como temos as evidências observacionais da existência do C4H2 e C6H6 na nebulosa CRL 618, sugerimos que este ambiente também possui o Naftaleno.

  15. Curva de rotação óptica de ESO-LV 5100550

    NASA Astrophysics Data System (ADS)

    Carvalho, D. B.; Soares, D. S. L.

    2003-08-01

    ESO-LV 5100550 é o membro mais fraco do par de galáxias austral SBG 357 (Soares et al. 1995). É classificada no catálogo RC3 como uma espiral ordinária de tipo inicial (early-type); porém, uma análise morfológica sugere que ela tenha uma grande barra. O objetivo do estudo é determinar sua cinemática de tal modo que possamos inferir mais a respeito de sua dinâmica, provavelmente perturbada, já que se espera que esteja sob forte influência da companheira ESO-LV 5100560. Apresentarei resultados parciais determinados a partir de espectros obtidos com o instrumento Double Spectrograph montado no telescópio Hale do Monte Palomar, EUA. As observações foram realizadas por D.S.L. Soares, P.M.V. Veiga e T.E. Nordgren, em 1998. Foram tomados espectros de fenda longa posicionada sobre a linha dos nodos do disco e ao longo da suposta barra. Os dados foram reduzidos com uso do pacote IRAF. Obtivemos o perfil de velocidades radiais na linha de visada ao longo das fendas e calculamos o desvio para o vermelho cosmológico do sistema, com base no espectro central. Determinamos as curvas de rotação deprojetadas, com base em cálculos para os valores teóricos esperados das componentes de velocidades puramente circulares em um disco inclinado. A inclinação do disco, dado fundamental nesta deprojeção, foi estimada através da média das elipticidades das isofotas mais externas.

  16. Modelo de atmosfera solar ajustado às observações do raio solar em 17GHz

    NASA Astrophysics Data System (ADS)

    Selhorst, C. L.; Silva, A. V. R.; Costa, J. E. R.

    2003-08-01

    O estudo das variações do raio solar durante o ciclo de atividades do Sol e das diferenças em relação à sua distribuição angular nos fornece informações importantes sobre as mudanças na estrutura da atmosfera solar. Neste trabalho foram analisados mais de 3600 mapas do Sol em 17 GHz obtidos pelo Rádio Heliógrafo de Nobeyama (NoRH), durante 1 ciclo de atividade solar (1992-2003). O raio solar foi definido no ponto onde a temperatura de brilho do mapa era equivalente à metade da temperatura do Sol calmo (temperatura mais comum no mapa).Em relação à sua variação ao longo do ciclo solar, o estudo foi dividido em duas partes: a) ajuste de uma circunferência a pontos distribuídos ao redor do Sol todo. Este estudo mostrou uma variação correlacionada com o ciclo de atividade do Sol. b) ajuste da circunferência a pontos situados somente nas regiões polares. Neste caso os resultados mostraram que o raio polar sofre pouca variação durante o ciclo, com tendência à anticorrelação com este. Além disto, a média do raio polar, durante o período analisado, foi 1" menor que o raio medido no Sol todo. Para estudar a distribuição angular do raio solar, comparamos a média da distribuição de 10 mapas no período de mínima atividade solar com a média de 10 mapas no período de máximo, este estudo mostrou um grande aumento do raio na região equatorial no período de máxima atividade solar. As medidas do raio foram usadas como um dos parâmetros para a criação de um modelo atmosférico (além da temperatura de brilho do Sol e do abrilhantamento do limbo observado), onde mostramos que um modelo atmosférico com a região de transição situada a 3500 km fornece um raio 5" menor que as medidas observacionais. Esta incompatibilidade do modelo com os dados observacionais foi contornada com a inclusão de espículas, estas fazem com que o raio solar aumente proporcionalmente à altura que estas atingem na atmosfera solar. A anticorrelação do raio

  17. [Diabetes mellitus and its influence on the success of endodontic treatment: a retrospective clinical study].

    PubMed

    Ferreira, Manuel Marques; Carrilho, Eunice; Carrilho, Francisco

    2014-01-01

    apical nos doentes diabéticos. Em relação à avaliação do sucesso dos tratamentos endodônticos analisados verificou-se que a percentagem de sucesso nos doentes diabéticos é mais baixa, embora não seja estatisticamente significativa. Por este motivo e dadas as limitações deste estudo, não se pode afirmar que doentes com diabetes mellitus têm maior predisposição para o desenvolvimento de lesõesperiradiculares ou que o sucesso dos tratamentos endodônticos nestes doentes esteja comprometido. É importante, no entanto, que sejam desenvolvidos mais estudos de forma a caracterizar as alterações pulpares e periradiculares e a avaliar a prevalência da periodontiteapical e sua progressão em doentes com diabetes mellitus.

  18. Blood species identification using Near-Infrared diffuse transmitted spectra and PLS-DA method

    NASA Astrophysics Data System (ADS)

    Zhang, Linna; Zhang, Shengzhao; Sun, Meixiu; Wang, Zhennan; Li, Hongxiao; Li, Yingxin; Li, Gang; Lin, Ling

    2016-05-01

    Blood species identification is of great significance for blood supervision and wildlife investigations. The current methods used to identify the blood species are destructive. Near-Infrared spectroscopy method is known for its non-invasive properties. In this research, we combined Near-Infrared diffuse transmitted spectra and Partial Least Square Discrimination Analysis (PLS-DA) to identify three blood species, including macaque, human and mouse. Blind test and external test were used to assess the PLS-DA model. The model performed 100% accuracy in its identification between three blood species. This approach does not require a specific knowledge of biochemical features for each individual class but relies on a spectroscopic statistical differentiation of the whole components. This is the first time to demonstrate Near-Infrared diffuse transmitted spectra have the ability to identify the species of origin of blood samples. The results also support a good potential of absorption and scattering spectroscopy for species identification in practical applications for real-time detection.

  19. Crystal Structure of the 25 kDa Subunit of Human Cleavage Factor I{m}

    SciTech Connect

    Coseno,M.; Martin, G.; Berger, C.; Gilmartin, G.; Keller, W.; Doublie, S.

    2008-01-01

    Cleavage factor Im is an essential component of the pre-messenger RNA 3'-end processing machinery in higher eukaryotes, participating in both the polyadenylation and cleavage steps. Cleavage factor Im is an oligomer composed of a small 25 kDa subunit (CF Im25) and a variable larger subunit of either 59, 68 or 72 kDa. The small subunit also interacts with RNA, poly(A) polymerase, and the nuclear poly(A)-binding protein. These protein-protein interactions are thought to be facilitated by the Nudix domain of CF Im25, a hydrolase motif with a characteristic {alpha}/{beta}/{alpha} fold and a conserved catalytic sequence or Nudix box. We present here the crystal structures of human CF Im25 in its free and diadenosine tetraphosphate (Ap4A) bound forms at 1.85 and 1.80 Angstroms, respectively. CF Im25 crystallizes as a dimer and presents the classical Nudix fold. Results from crystallographic and biochemical experiments suggest that CF Im25 makes use of its Nudix fold to bind but not hydrolyze ATP and Ap4A. The complex and apo protein structures provide insight into the active oligomeric state of CF Im and suggest a possible role of nucleotide binding in either the polyadenylation and/or cleavage steps of pre-messenger RNA 3'-end processing.

  20. Molecular cloning and sequence determination of DA strain of Theiler's murine encephalomyelitis viruses.

    PubMed

    Ohara, Y; Stein, S; Fu, J L; Stillman, L; Klaman, L; Roos, R P

    1988-05-01

    Theiler's murine encephalomyelitis viruses (TMEV) belong to the Picornaviridae, and are divided into two subgroups. TO subgroup strains produce a persistent demyelinating central nervous system infection in mice, while GDVII subgroup strains cause acute polioencephalomyelitis. We generated three overlapping clones of the genome of DA strain, a member of TO subgroup. Sequence analysis revealed that the genome is 8093 nucleotides long with a poly(A) tail. The 5' noncoding region stretches from nucleotides 1 to 1065 and lacks a poly(C) tract. The open reading frame stretches from 1066 to 7968 and encodes 2301 amino acids. DA strain sequence is more closely related to members of the Cardiovirus genus than to members of other Picornavirus genera. Comparison with sequence of BeAn strain, another TO subgroup strain, showed that the P1 area has the greatest number of differences, while the noncoding regions are more well-conserved. The three overlapping clones will be important in recombinant infectious cDNA studies between strains of both subgroups.

  1. Age of ultramafic high-K rocks from Planalto da Serra (Mato Grosso, Brazil)

    NASA Astrophysics Data System (ADS)

    De Min, Angelo; Hendriks, Bart; Slejko, Francesca; Comin-Chiaramonti, Piero; Girardi, Vicente; Ruberti, Excelso; Gomes, Celso B.; Neder, Renato D.; Pinho, Francisco C.

    2013-01-01

    The Planalto da Serra ultramafic-alkaline rocks are represented by several plugs, lava-flows and N60-80E trending dykes which are mainly represented by glimmerites, harzburgites and carbonatites. These rocks intrude the Cryogenian basal unit of the Northern Paraguay Belt in the Rio dos Cavalos rift (southeastern part of the Amazonian Craton). 40Ar/39Ar dating on the phlogopite from glimmeritic dykes and plugs yielded an age of ca. 600 Ma, which is consistent with Rb/Sr and Sm/Nd determinations on the other rock-types. These age determinations make it difficult to establish a geochronological and genetic relationship between the Planalto da Serra rocks and the Cretaceous bodies of the "Azimuth 125° Lineament", and it suggests there is a relationship between its emplacement and the beginning of the extensional tectonics responsible for the separation of the Amazonian and Laurentia Cratons. This age also indicates that the onset of the deformation of the Cuiaba Group may be older than 600 Ma.

  2. UniDA: Uniform Device Access Framework for Human Interaction Environments

    PubMed Central

    Varela, Gervasio; Paz-Lopez, Alejandro; Becerra, Jose Antonio; Vazquez-Rodriguez, Santiago; Duro, Richard José

    2011-01-01

    Human interaction environments (HIE) must be understood as any place where people carry out their daily life, including their work, family life, leisure and social life, interacting with technology to enhance or facilitate the experience. The integration of technology in these environments has been achieved in a disorderly and incompatible way, with devices operating in isolated islands with artificial edges delimited by the manufacturers. In this paper we are presenting the UniDA framework, an integral solution for the development of systems that require the integration and interoperation of devices and technologies in HIEs. It provides developers and installers with a uniform conceptual framework capable of modelling an HIE, together with a set of libraries, tools and devices to build distributed instrumentation networks with support for transparent integration of other technologies. A series of use case examples and a comparison to many of the existing technologies in the field has been included in order to show the benefits of using UniDA. PMID:22163700

  3. On the purported discovery of the bronchial circulation by Leonardo da Vinci.

    PubMed

    Mitzner, W; Wagner, E

    1992-09-01

    Among modern physiologists and anatomists, there has been a nearly universal acceptance that Leonardo da Vinci was the first to identify the anatomy of the bronchial circulation. However, because of certain ambiguities in both his anatomic drawing that was supposed to have shown this circulation and the accompanying descriptive text, we questioned whether he really could have been the first to discover this small but important vasculature. To address this question, we set out to repeat Leonardo's dissections in the ox. We reasoned that perhaps the normally tiny bronchial vessels would be considerably more noticeable in this very large species. Our dissections, however, failed to provide any evidence that Leonardo's drawing was that of the bronchial circulation. Furthermore we observed a set of distinct small pulmonary veins to the left upper and right middle lobes that Leonardo, given his lack of understanding of the function of the lung and its circulation, could have easily mistaken for a separate circulation. We thus conclude that Leonardo da Vinci did not describe the anatomy of the bronchial circulation. We believe that the first person to clearly and unequivocally describe the anatomy of this circulation was the Dutch Professor of Anatomy and Botany, Frederich Ruysch.

  4. VaccineDA: Prediction, design and genome-wide screening of oligodeoxynucleotide-based vaccine adjuvants.

    PubMed

    Nagpal, Gandharva; Gupta, Sudheer; Chaudhary, Kumardeep; Dhanda, Sandeep Kumar; Prakash, Satya; Raghava, Gajendra P S

    2015-07-27

    Immunomodulatory oligodeoxynucleotides (IMODNs) are the short DNA sequences that activate the innate immune system via toll-like receptor 9. These sequences predominantly contain unmethylated CpG motifs. In this work, we describe VaccineDA (Vaccine DNA adjuvants), a web-based resource developed to design IMODN-based vaccine adjuvants. We collected and analyzed 2193 experimentally validated IMODNs obtained from the literature. Certain types of nucleotides (e.g., T, GT, TC, TT, CGT, TCG, TTT) are dominant in IMODNs. Based on these observations, we developed support vector machine-based models to predict IMODNs using various compositions. The developed models achieved the maximum Matthews Correlation Coefficient (MCC) of 0.75 with an accuracy of 87.57% using the pentanucleotide composition. The integration of motif information further improved the performance of our model from the MCC of 0.75 to 0.77. Similarly, models were developed to predict palindromic IMODNs and attained a maximum MCC of 0.84 with the accuracy of 91.94%. These models were evaluated using a five-fold cross-validation technique as well as validated on an independent dataset. The models developed in this study were integrated into VaccineDA to provide a wide range of services that facilitate the design of DNA-based vaccine adjuvants (http://crdd.osdd.net/raghava/vaccineda/).

  5. Leonardo da Vinci and Andreas Vesalius; the shoulder girdle and the spine, a comparison.

    PubMed

    Ganseman, Y; Broos, P

    2008-01-01

    Leonardo Da Vinci and Andreas Vesalius were two important renaissance persons; Vesalius was a surgeon-anatomist who delivered innovative work on the study of the human body, Leonardo da Vinci was an artist who delivered strikingly accurate and beautiful drawings on the human body. Below we compare both masters with regard to their knowledge of the working of the muscles, their method and system of dissection and their system and presentation of the drawings. The investigation consisted of a comparison between both anatomists, in particular concerning their study on the shoulder girdle and spine, by reviewing their original work as well as already existing literature on this subject. The investigation led to the conclusion that the drawings mentioned meant a change in history, and were of high quality, centuries ahead of their time. Both were anatomists, both were revolutionary, only one changed history at the moment itself, while the other changed history centuries later. Leonardo has made beautiful drawings that are at a match with the drawings of today or are even better. Vesalius set the start for medicine as a science as it is until this day. Their lives differed as strongly as their impact. In the light of their time, the achievement they made was extraordinary. PMID:18807610

  6. 3D-printing of transparent bio-microfluidic devices in PEG-DA.

    PubMed

    Urrios, Arturo; Parra-Cabrera, Cesar; Bhattacharjee, Nirveek; Gonzalez-Suarez, Alan M; Rigat-Brugarolas, Luis G; Nallapatti, Umashree; Samitier, Josep; DeForest, Cole A; Posas, Francesc; Garcia-Cordero, José L; Folch, Albert

    2016-06-21

    The vast majority of microfluidic systems are molded in poly(dimethylsiloxane) (PDMS) by soft lithography due to the favorable properties of PDMS: biocompatible, elastomeric, transparent, gas-permeable, inexpensive, and copyright-free. However, PDMS molding involves tedious manual labor, which makes PDMS devices prone to assembly failures and difficult to disseminate to research and clinical settings. Furthermore, the fabrication procedures limit the 3D complexity of the devices to layered designs. Stereolithography (SL), a form of 3D-printing, has recently attracted attention as a way to customize the fabrication of biomedical devices due to its automated, assembly-free 3D fabrication, rapidly decreasing costs, and fast-improving resolution and throughput. However, existing SL resins are not biocompatible and patterning transparent resins at high resolution remains difficult. Here we report procedures for the preparation and patterning of a transparent resin based on low-MW poly(ethylene glycol) diacrylate (MW 250) (PEG-DA-250). The 3D-printed devices are highly transparent and cells can be cultured on PEG-DA-250 prints for several days. This biocompatible SL resin and printing process solves some of the main drawbacks of 3D-printed microfluidic devices: biocompatibility and transparency. In addition, it should also enable the production of non-microfluidic biomedical devices.

  7. Leonardo da Vinci and Andreas Vesalius; the shoulder girdle and the spine, a comparison.

    PubMed

    Ganseman, Y; Broos, P

    2008-01-01

    Leonardo Da Vinci and Andreas Vesalius were two important renaissance persons; Vesalius was a surgeon-anatomist who delivered innovative work on the study of the human body, Leonardo da Vinci was an artist who delivered strikingly accurate and beautiful drawings on the human body. Below we compare both masters with regard to their knowledge of the working of the muscles, their method and system of dissection and their system and presentation of the drawings. The investigation consisted of a comparison between both anatomists, in particular concerning their study on the shoulder girdle and spine, by reviewing their original work as well as already existing literature on this subject. The investigation led to the conclusion that the drawings mentioned meant a change in history, and were of high quality, centuries ahead of their time. Both were anatomists, both were revolutionary, only one changed history at the moment itself, while the other changed history centuries later. Leonardo has made beautiful drawings that are at a match with the drawings of today or are even better. Vesalius set the start for medicine as a science as it is until this day. Their lives differed as strongly as their impact. In the light of their time, the achievement they made was extraordinary.

  8. The uncatchable smile in Leonardo da Vinci's La Bella Principessa portrait.

    PubMed

    Soranzo, Alessandro; Newberry, Michelle

    2015-08-01

    A portrait of uncertain origin recently came to light which, after extensive research and examination, was shown to be that rarest of things: a newly discovered Leonardo da Vinci painting entitled La Bella Principessa. This research presents a new illusion which is similar to that identified in the Mona Lisa; La Bella Principessa's mouth appears to change slant depending on both the Viewing Distance and the Level of Blur applied to a digital version of the portrait. Through a series of psychophysics experiments, it was found that a perceived change in the slant of the La Bella Principessa's mouth influences her expression of contentment thus generating an illusion that we have coined the "uncatchable smile". The elusive quality of the Mona Lisa's smile has been previously reported (Science, 290 (2000) 1299) and so the existence of a similar illusion in a portrait painted prior to the Mona Lisa becomes more interesting. The question remains whether Leonardo da Vinci intended this illusion. In any case, it can be argued that the ambiguity created adds to the portrait's allure.

  9. [History of robotics: from Archytas of Tarentum until da Vinci robot. (Part I)].

    PubMed

    Sánchez Martín, F M; Millán Rodríguez, F; Salvador Bayarri, J; Palou Redorta, J; Rodríguez Escovar, F; Esquena Fernández, S; Villavicencio Mavrich, H

    2007-02-01

    Robotic surgery is the newst technologic option in urology. To understand how new robots work is interesting to know their history. The desire to design machines imitating humans continued for more than 4000 years. There are references to King-su Tse (clasic China) making up automaton at 500 a. C. Archytas of Tarentum (at around 400 a.C.) is considered the father of mechanical engineering, and one of the occidental robotics classic referents. Heron of Alexandria, Hsieh-Fec, Al-Jazari, Roger Bacon, Juanelo Turriano, Leonardo da Vinci, Vaucanson o von Kempelen were robot inventors in the middle age, renaissance and classicism. At the XIXth century, automaton production underwent a peak and all engineering branches suffered a great development. At 1942 Asimov published the three robotics laws, based on mechanics, electronics and informatics advances. At XXth century robots able to do very complex self governing works were developed, like da Vinci Surgical System (Intuitive Surgical Inc, Sunnyvale, CA, USA), a very sophisticated robot to assist surgeons.

  10. Development of noSQL data storage for the ATLAS PanDA Monitoring System

    NASA Astrophysics Data System (ADS)

    Ito, H.; Potekhin, M.; Wenaus, T.

    2012-12-01

    For several years the PanDA Workload Management System has been the basis for distributed production and analysis for the ATLAS experiment at the LHC. Since the start of data taking PanDA usage has ramped up steadily, typically exceeding 500k completed jobs/day by June 2011. The associated monitoring data volume has been rising as well, to levels that present a new set of challenges in the areas of database scalability and monitoring system performance and efficiency. These challenges are being met with an R&D effort aimed at implementing a scalable and efficient monitoring data storage based on a noSQL solution (Cassandra). We present our motivations for using this technology, as well as data design and the techniques used for efficient indexing of the data. We also discuss the hardware requirements as they were determined by testing with actual data and realistic rate of queries. In conclusion, we present our experience with operating a Cassandra cluster over an extended period of time and with data load adequate for planned application.

  11. 3D-printing of transparent bio-microfluidic devices in PEG-DA.

    PubMed

    Urrios, Arturo; Parra-Cabrera, Cesar; Bhattacharjee, Nirveek; Gonzalez-Suarez, Alan M; Rigat-Brugarolas, Luis G; Nallapatti, Umashree; Samitier, Josep; DeForest, Cole A; Posas, Francesc; Garcia-Cordero, José L; Folch, Albert

    2016-06-21

    The vast majority of microfluidic systems are molded in poly(dimethylsiloxane) (PDMS) by soft lithography due to the favorable properties of PDMS: biocompatible, elastomeric, transparent, gas-permeable, inexpensive, and copyright-free. However, PDMS molding involves tedious manual labor, which makes PDMS devices prone to assembly failures and difficult to disseminate to research and clinical settings. Furthermore, the fabrication procedures limit the 3D complexity of the devices to layered designs. Stereolithography (SL), a form of 3D-printing, has recently attracted attention as a way to customize the fabrication of biomedical devices due to its automated, assembly-free 3D fabrication, rapidly decreasing costs, and fast-improving resolution and throughput. However, existing SL resins are not biocompatible and patterning transparent resins at high resolution remains difficult. Here we report procedures for the preparation and patterning of a transparent resin based on low-MW poly(ethylene glycol) diacrylate (MW 250) (PEG-DA-250). The 3D-printed devices are highly transparent and cells can be cultured on PEG-DA-250 prints for several days. This biocompatible SL resin and printing process solves some of the main drawbacks of 3D-printed microfluidic devices: biocompatibility and transparency. In addition, it should also enable the production of non-microfluidic biomedical devices. PMID:27217203

  12. Domain Organization in the 54-kDa Subunit of the Chloroplast Signal Recognition Particle.

    PubMed

    Henderson, Rory C; Gao, Feng; Jayanthi, Srinivas; Kight, Alicia; Sharma, Priyanka; Goforth, Robyn L; Heyes, Colin D; Henry, Ralph L; Suresh Kumar, Thallapuranam Krishnaswamy

    2016-09-20

    Chloroplast signal recognition particle (cpSRP) is a heterodimer composed of an evolutionarily conserved 54-kDa GTPase (cpSRP54) and a unique 43-kDa subunit (cpSRP43) responsible for delivering light-harvesting chlorophyll binding protein to the thylakoid membrane. While a nearly complete three-dimensional structure of cpSRP43 has been determined, no high-resolution structure is yet available for cpSRP54. In this study, we developed and examined an in silico three-dimensional model of the structure of cpSRP54 by homology modeling using cytosolic homologs. Model selection was guided by single-molecule Förster resonance energy transfer experiments, which revealed the presence of at least two distinct conformations. Small angle x-ray scattering showed that the linking region among the GTPase (G-domain) and methionine-rich (M-domain) domains, an M-domain loop, and the cpSRP43 binding C-terminal extension of cpSRP54 are predominantly disordered. Interestingly, the linker and loop segments were observed to play an important role in organizing the domain arrangement of cpSRP54. Further, deletion of the finger loop abolished loading of the cpSRP cargo, light-harvesting chlorophyll binding protein. These data highlight important structural dynamics relevant to cpSRP54's role in the post- and cotranslational signaling processes. PMID:27653474

  13. Integration of Globus Online with the ATLAS PanDA Workload Management System

    NASA Astrophysics Data System (ADS)

    Contreras, C.; Deng, W.; Maeno, T.; Nilsson, P.; Potekhin, M.

    2012-12-01

    The PanDA Workload Management System is the basis for distributed production and analysis for the ATLAS experiment at the LHC. In this role, it relies on sophisticated dynamic data movement facilities developed in ATLAS. In certain scenarios, such as small research teams in ATLAS Tier-3 sites and non-ATLAS Virtual Organizations, the overhead of installation and operation of these components makes their use not very cost effective. Globus Online is an emerging new tool from the Globus Alliance, which already proved popular within the research community. It provides the users with fast and robust file transfer capabilities that can also be managed from a Web interface, and in addition to grid sites, can have individual workstations and laptops serving as data transmission endpoints. We will describe the integration of the Globus Online functionality into the PanDA suite of software, in order to give more flexibility in choosing the method of data transfer to ATLAS Tier-3 and Open Science Grid (OSG) users.

  14. Cladocera Chydoridae of high altitude water bodies (Serra da Mantiqueira), in Brazil.

    PubMed

    Santos Wisniewski, M J; Rocha, O; Güntzel, A M; Matsumura-Tundisi, T

    2002-11-01

    The diversity of species of Cladocera, Chydoridae in the water bodies belonging to the water management unity of Serra da Mantiqueira, was studied inside the BIOTA/FAPESP Program. A total of 21 water bodies were analyzed and the sampling was carried out between 9th and 11th September, 1999. Horizontal and vertical net hauls were taken at littoral and limnetic region in each lake. Most water bodies in Serra da Mantiqueira unity are small, shallow, and densely colonized by macrophytes. Most species of Cladocera found belong to the Chydoridae family, being typical inhabitants of the littoral zone, living associated with macrophytes. A total of 12 species were found, 9 of the subfamily Aloninae and 3 Chydorinae. In more than half of the water bodies the relative abundance of chydorids reached 100%, and they were dominant in most of the others, with few exceptions. Alona rustica was the most frequent species, occurring in 62% of the water bodies. The results evidenced the importance of the littoral zone as propitious habitats for the species of Chydoridae and contributing significantly to the diversity of Cladocera as a whole.

  15. Jacob Mendez DaCosta: medical teacher, clinician, and clinical investigator.

    PubMed

    Wooley, C F

    1982-11-01

    J. M. DaCosta, a scholarly, well-trained and observant clinician, was recognized during his lifetime as a well-known authority on physical diagnosis and had an unexcelled reputation as a clinical teacher. Chairman of Medicine at the Jefferson Medical College for 19 years, president of the College of Physicians of Philadelphia in 1884 and again in 1895, he was one of the original members of the Association of American Physicians and its president in 1897. Earlier in his career, his extensive Civil War study of "a form of cardiac malady common among soldiers . . . the study of which is equally interesting to the civil practitioner" was described in his 1871 paper "On Irritable Heart; A Clinical Study of a Form of Functional Cardiac Disorder and Its Consequences." Soon labeled DaCosta's syndrome, the irritable heart lineage can be traced through the soldier's heart, the effort syndrome, and neurocirculatory asthenia in World War I, anxiety neurosis in World War II, and the mitral valve prolapse syndrome in the second half of the 20th century.

  16. Otimização de procedimento de manobra para indução de reentrada de um satélite retornável

    NASA Astrophysics Data System (ADS)

    Schulz, W.; Suarez, M.

    2003-08-01

    Veículos espaciais que retornam à Terra passam por regimes de velocidade e condições de vôo distintos. Estas diferenças dificultam sua concepção aerodinâmica e o planejamento de seu retorno. A partir de uma proposta de um veículo orbital retornável (satélite SARA, em desenvolvimento no IAE/CTA) para realização de experimentos científicos e tecnológicos em ambiente de baixa gravidade, surge a necessidade de realizarem-se estudos considerando-se os aspectos relativos à sua aerodinâmica. Após o lançamento, o veículo deve permanecer em órbita pelo tempo necessário para a condução de experimentos, sendo depois direcionado à Terra e recuperado em solo. A concepção aerodinâmica é de importância para o vôo em suas diversas fases e deve considerar aspectos relativos à estabilização Aerodinâmica e ao arrasto atmosférico, sendo este último de importância crucial na análise do aquecimento a ser enfrentado. A manobra de retorno inclui considerações sobre as condições atmosféricas e dinâmica de reentrada, devendo ser calculada de forma mais precisa possível. O trabalho proposto avalia estudos da dinâmica de vôo de um satélite recuperável considerando aspectos relativos à determinação orbital com GPS, técnica utilizada com sucesso na CONAE, e seu comportamento aerodinâmico em vôo balístico de retorno, com ênfase em sua fase de reentrada atmosférica. Busca-se otimizar a manobra de reentrada de tal forma que a utilização do sistema GPS garanta minimizar a área de impacto com o solo.

  17. Pathogenesis of early and late disease in mice infected with Theiler's virus, using intratypic recombinant GDVII/DA viruses.

    PubMed Central

    Rodriguez, M; Roos, R P

    1992-01-01

    Intratypic recombinant Theiler's viruses prepared between GDVII and DA strains were used to identify genomic sequences important in neurovirulence, virus persistence, and demyelination and to clarify the mechanisms involved in disease induction. The coding region between 1B and 2C of the highly virulent GDVII strain contains a determinant partly responsible for neurovirulence (early paralysis and death) which correlates with elevated levels of infectious virus and the presence of virus antigen within neurons of the brain stem and gray matter of the spinal cord. Both the GDVII and the DA strains of virus contain genetic determinants for late demyelination in spinal cord. However, quantitative analysis of demyelination produced by recombinant GDVII/DA viruses suggest that multiple gene segments influence the number and extent of demyelinating lesions. Images PMID:1727485

  18. Food residue recycling by swine breeders in a developing economy: a case study in Da Nang, Viet Nam.

    PubMed

    Kato, Takaaki; Pham, Dung Thi Xuan; Hoang, Hai; Xue, Yonghai; Tran, Quang Van

    2012-12-01

    This study provides a detailed description of food residue collection by swine breeders in Da Nang, Viet Nam. In January 2011, the study surveyed 30 swine breeders in two villages with respect to locations, methods, prices, quantities, and prospects for food residue collection. The sampled swine breeders regularly visited 55 locations in central Da Nang to collect raw food residue. They then transferred the food residue to their piggeries, boiled it, and fed it to their swine. A regression analysis revealed that the total amount of food residue collected by a farm depends on the number of swine in the farm and the number of collections made per day. Swine breeders in Da Nang were estimated to collect 26.3 metric tons of organic waste per day, which amounted to 4.1% of domestic waste collected by the local government. Among the sampled swine breeders, 93% answered that they would continue using food residue for the next five years.

  19. Degradation of dimethyl carboxylic phthalate ester by Burkholderia cepacia DA2 isolated from marine sediment of South China Sea.

    PubMed

    Wang, Yali; Yin, Bo; Hong, Yiguo; Yan, Yan; Gu, Ji-Dong

    2008-11-01

    Burkholderia cepacia DA2, isolated from marine sediment of the South China Sea, is capable of utilizing dimethyl phthalate (DMP) as the sole source of carbon and energy. During the transformation of DMP in batch culture, its corresponding degradation intermediates were identified as monomethyl phthalate (MMP) and phthalate acid (PA) sequentially over the time of incubation. The biodegradation biochemical pathway of DMP was DMP to MMP and then to PA before mineralization. Degradation of DMP by B. cepacia DA2 was also dependent upon DMP-induction, and the initial concentrations of DMP affected the degradation rate. Degradation kinetics fit well with the modified Gompertz model. The optimum pH and salinity was 6.0 and < 5 per thousand, respectively, for DMP degradation by B. cepacia DA2. This study showed that the indigenous microorganisms of the deep-ocean sediments are capable of DMP degradation completely.

  20. L151-81A/B - A unique white dwarf binary with DB and DA components

    NASA Technical Reports Server (NTRS)

    Oswalt, Terry D.; Hintzen, Paul M.; Liebert, James W.; Sion, Edward M.

    1988-01-01

    Spectroscopic observations of the wide binary L151-81A/B reveal that both components are white dwarfs with spectral types DB3 and DA4, the first such binary identified in a comprehensive survey of Luyten and Giclas common proper motion pairs. Assuming log g = 8, measurements of the helium line profiles in the DB primary yield Teff = 16,000 + or - 2,000, while the hydrogen profiles for the DA companion yield Teff =12,000 + or - 2,000. The existence of a helium-rich/hydrogen-rich degenerate pair offers several interesting tests of theoretical white dwarf formation channels and surface abundance evolution.

  1. Cloning and sequencing of 28 kDa outer membrane protein gene of Brucella melitensis Rev. 1.

    PubMed

    Chaudhuri, Pallab; Kumar, S Vinoth; Prasad, Rajeev; Srivastava, S K; Yadav, M P

    2005-09-01

    Brucella melitensis is an organism of paramount zoonotic importance. The 28 kDa outer membrane protein (OMP) is one of the immunodominant antigens of B. melitensis. The gene encoding 28 kDa OMP (omp28) has been amplified from B. melitensis Rev. 1 strain. A PCR product of 753 bp, encoding complete omp28 gene of B. melitensis, was obtained. The gene was further cloned and sequenced. The nucleotide sequence of B. melitensis Rev. 1 strain showed substitution of 2 nucleotides from that of 16M strain.

  2. Hopf-Ranãda linked and knotted light beam solution viewed as a null electromagnetic field.

    PubMed

    Besieris, Ioannis M; Shaarawi, Amr M

    2009-12-15

    The Hopf-Ranãda linked and knotted light beam solution, which has been interpreted physically and extended analytically by Irvine and Bouwmeester recently, is viewed in this Letter as a null electromagnetic field. It is shown, in particular, that the Hopf-Ranãda solution is a variant of a luminal null electromagnetic wave due originally to Robinson and Troutman and reported by Bialynicki-Birula recently. This analogy is motivated by means of a method due to Whittaker and Bateman, and a relationship to well-known scalar luminal localized waves is examined. PMID:20016647

  3. Genome Sequence of the Psychrophilic Bacterium Tenacibaculum ovolyticum Strain da5A-8 Isolated from Deep Seawater

    PubMed Central

    Zhai, Zhenyu; Komatsu, Ayumi; Shibayama, Keigo

    2016-01-01

    Some bacterial species of the genus Tenacibaculum, including Tenacibaculum ovolyticum, have been known as fish pathogens in the sea. So far, the only published genome sequence for this genus is for Tenacibaculum dicentrarchi, which could also be a fish pathogen. Strain da5A-8, showing 100% identity to the 16S rRNA gene sequence of T. ovolyticum DSM 18103T, was isolated from seawater at a depth of 344 m in Kochi, Japan, and grew optimally at 10 to 20°C. The genome sequence of strain da5A-8 revealed the possible virulence genes commonly observed in the genus Tenacibaculum. PMID:27365358

  4. Purification, characterization and allergenicity assessment of 26kDa protein, a major allergen from Cicer arietinum.

    PubMed

    Verma, Alok Kumar; Sharma, Akanksha; Kumar, Sandeep; Gupta, Rinkesh Kumar; Kumar, Dinesh; Gupta, Kriti; Giridhar, B H; Das, Mukul; Dwivedi, Premendra D

    2016-06-01

    Chickpea (CP), a legume of the family Fabaceae, is an important nutrient-rich food providing protein, essential amino acids, vitamins, dietary fibre, and minerals. Unfortunately, several IgE-binding proteins in CP have been detected that are responsible for allergic manifestations in sensitized population. Therefore, the prevalence of CP induced allergy prompted us towards purification, characterization and allergenicity assessment of a major ∼26kDa protein from chickpea crude protein extract (CP-CPE). Purification of CP 26kDa protein was done using a combination of fractionation and anion exchange chromatography. This protein was further characterized as "Chain A, crystal structure of a plant albumin" from Cicer arietinum with Mol wt 25.8kDa by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Further, allergenic potential of purified 25.8kDa protein was assessed using in vivo and in vitro model. Purified protein showed IgE-binding capacity with sensitized BALB/c mice and CP allergic patient's sera. Enhanced levels of specific and total IgE, MCP-1, MCPT-1, myeloperoxidase, histamine, prostaglandin D2, and cysteinyl leukotriene were found in sera of mice treated with CP ∼26kDa protein. Further, expressions of Th2 cytokines (i.e. IL-4, IL-5, IL-13), transcription factors (i.e. GATA-3, STAT-6, SOCS-3) and mast cell signaling proteins (Lyn, cFgr, Syk, PLC-γ2, PI-3K, PKC) were also found increased at mRNA and protein levels in the intestines of mice treated with CP ∼26kDa protein. In addition, enhanced release of β-hexosaminidase, histamine, cysteinyl leukotriene and prostaglandin D2 were observed in RBL2H3 cell line when treated (125μg) with CP 26kDa protein. Conclusively, in vivo and in vitro studies revealed the allergenic potential of purified CP 26kDa protein. Being a potential allergen, plant albumin may play a pivotal role in CP induced allergenicity. Current study will be helpful for better development of therapeutic approaches to

  5. The Understanding of Astronomy Concepts by Students from Basic Education of a Public School. (Spanish Title: El Entendimiento de Conceptos de Aastronmía Por Los Alumnos de Educación Básica en Una Escuela Pública.) O Entendimento de Conceitos de Astronomia Por Alunos da Educação Básica: O Caso de Uma Escola Pública Brasileira

    NASA Astrophysics Data System (ADS)

    Iria Machado, Daniel; dos Santos, Carlos

    2011-07-01

    movimiento de la Luna, el movimiento aparente del Sol sobre la esfera celeste, los eclipses, las dimensiones y las distancias en el Universo, el brillo de las estrellas y su observación de la Tierra. Si bien ha habido un pequeño progreso en la proporción de respuestas aceptables científicamente cuando se cotejó el octavo grado de la enseñanza primaria con el quinto, y el último año de la enseñanza secundaria con el primero, se observó un predominio de las concepciones alternativas en relación a la mayoría de los temas explorados, que continuó hasta los últimos años de la educación básica. Una comparación con los datos encontrados en investigaciones realizadas en otros contextos socioculturales revelaron, en muchos aspectos, conceptos y dificultades similares expresadas por los estudiantes. Apresentam-se os resultados de uma investigação sobre a compreensão de conceitos astronômicos básicos, da qual participaram 561 estudantes da quinta série do Ensino Fundamental ao terceiro ano do Ensino Médio de uma escola pública da cidade de Foz do Iguaçu. Um teste com 20 questões de múltipla escolha foi aplicado para identificar as concepções mais comuns expressadas pelos alunos. Esse instrumento de coleta de dados foi elaborado com base na literatura sobre concepções alternativas e abordou os seguintes temas: o ciclo dia-noite; os fusos horários; as estações do ano; as fases da Lua; o movimento da Lua; o movimento aparente do Sol na esfera celeste; os eclipses; as dimensões e distâncias no Universo; o brilho das estrelas e sua observação da Terra. Embora se tenha verificado um pequeno progresso na proporção de respostas cientificamente aceitáveis ao se cotejar a oitava série do Ensino Fundamental com a quinta, e a terceira série do Ensino Médio com a primeira, houve o predomínio de concepções alternativas em relação à maior parte dos temas explorados, as quais persistiram até o último ano da Educação Básica. A comparação com

  6. The Understanding of Astronomy Concepts by Students from Basic Education of a Public School. (Spanish Title: El Entendimiento de Conceptos de Aastronmía Por Los Alumnos de Educación Básica en Una Escuela Pública.) O Entendimento de Conceitos de Astronomia Por Alunos da Educação Básica: O Caso de Uma Escola Pública Brasileira

    NASA Astrophysics Data System (ADS)

    Iria Machado, Daniel; dos Santos, Carlos

    2011-07-01

    movimiento de la Luna, el movimiento aparente del Sol sobre la esfera celeste, los eclipses, las dimensiones y las distancias en el Universo, el brillo de las estrellas y su observación de la Tierra. Si bien ha habido un pequeño progreso en la proporción de respuestas aceptables científicamente cuando se cotejó el octavo grado de la enseñanza primaria con el quinto, y el último año de la enseñanza secundaria con el primero, se observó un predominio de las concepciones alternativas en relación a la mayoría de los temas explorados, que continuó hasta los últimos años de la educación básica. Una comparación con los datos encontrados en investigaciones realizadas en otros contextos socioculturales revelaron, en muchos aspectos, conceptos y dificultades similares expresadas por los estudiantes. Apresentam-se os resultados de uma investigação sobre a compreensão de conceitos astronômicos básicos, da qual participaram 561 estudantes da quinta série do Ensino Fundamental ao terceiro ano do Ensino Médio de uma escola pública da cidade de Foz do Iguaçu. Um teste com 20 questões de múltipla escolha foi aplicado para identificar as concepções mais comuns expressadas pelos alunos. Esse instrumento de coleta de dados foi elaborado com base na literatura sobre concepções alternativas e abordou os seguintes temas: o ciclo dia-noite; os fusos horários; as estações do ano; as fases da Lua; o movimento da Lua; o movimento aparente do Sol na esfera celeste; os eclipses; as dimensões e distâncias no Universo; o brilho das estrelas e sua observação da Terra. Embora se tenha verificado um pequeno progresso na proporção de respostas cientificamente aceitáveis ao se cotejar a oitava série do Ensino Fundamental com a quinta, e a terceira série do Ensino Médio com a primeira, houve o predomínio de concepções alternativas em relação à maior parte dos temas explorados, as quais persistiram até o último ano da Educação Básica. A comparação com

  7. A 7-kDa region of the bacteriophage T7 gene 4 protein is required for primase but not for helicase activity.

    PubMed

    Bernstein, J A; Richardson, C C

    1988-01-01

    Bacteriophage T7 gene 4 protein, purified from phage-infected cells, consists of a mixture of 56- and 63-kDa species that provides helicase and primase activities required for T7 DNA replication. The 56-kDa species has been purified independently of the colinear 63-kDa species. Like a mixture of the two proteins, the 56-kDa protein binds single-stranded DNA in the presence of dTTP, catalyzes DNA-dependent hydrolysis of dTTP, and has helicase activity. In contrast to the mixture, the 56-kDa protein cannot catalyze template-dependent RNA primer synthesis. In the absence of a DNA template, both the 56-kDa protein and the mixture of the two species synthesize low levels of diribonucleotide. A putative "zinc finger" present near the amino terminus of the 63-kDa protein but absent from the 56-kDa protein may play a major role in the recognition of primase sites in the template.

  8. A 7-kDa region of the bacteriophage T7 gene 4 protein is required for primase but not for helicase activity.

    PubMed Central

    Bernstein, J A; Richardson, C C

    1988-01-01

    Bacteriophage T7 gene 4 protein, purified from phage-infected cells, consists of a mixture of 56- and 63-kDa species that provides helicase and primase activities required for T7 DNA replication. The 56-kDa species has been purified independently of the colinear 63-kDa species. Like a mixture of the two proteins, the 56-kDa protein binds single-stranded DNA in the presence of dTTP, catalyzes DNA-dependent hydrolysis of dTTP, and has helicase activity. In contrast to the mixture, the 56-kDa protein cannot catalyze template-dependent RNA primer synthesis. In the absence of a DNA template, both the 56-kDa protein and the mixture of the two species synthesize low levels of diribonucleotide. A putative "zinc finger" present near the amino terminus of the 63-kDa protein but absent from the 56-kDa protein may play a major role in the recognition of primase sites in the template. Images PMID:2829184

  9. DA-1229, a dipeptidyl peptidase IV inhibitor, protects against renal injury by preventing podocyte damage in an animal model of progressive renal injury.

    PubMed

    Eun Lee, Jee; Kim, Jung Eun; Lee, Mi Hwa; Song, Hye Kyoung; Ghee, Jung Yeon; Kang, Young Sun; Min, Hye Sook; Kim, Hyun Wook; Cha, Jin Joo; Han, Jee Young; Han, Sang Youb; Cha, Dae Ryong

    2016-05-01

    Although dipeptidyl peptidase IV (DPPIV) inhibitors are known to have renoprotective effects, the mechanism underlying these effects has remained elusive. Here we investigated the effects of DA-1229, a novel DPPIV inhibitor, in two animal models of renal injury including db/db mice and the adriamycin nephropathy rodent model of chronic renal disease characterized by podocyte injury. For both models, DA-1229 was administered at 300 mg/kg/day. DPPIV activity in the kidney was significantly higher in diabetic mice compared with their nondiabetic controls. Although DA-1229 did not affect glycemic control or insulin resistance, DA-1229 did improve lipid profiles, albuminuria and renal fibrosis. Moreover, DA-1229 treatment resulted in decreased urinary excretion of nephrin, decreased circulating and kidney DPPIV activity, and decreased macrophage infiltration in the kidney. In adriamycin-treated mice, DPPIV activity in the kidney and urinary nephrin loss were both increased, whereas glucagon-like peptide-1 concentrations were unchanged. Moreover, DA-1229 treatment significantly improved proteinuria, renal fibrosis and inflammation associated with decreased urinary nephrin loss, and kidney DPP4 activity. In cultured podocytes, DA-1229 restored the high glucose/angiotensin II-induced increase of DPPIV activity and preserved the nephrin levels in podocytes. These findings suggest that activation of DPPIV in the kidney has a role in the progression of renal disease, and that DA-1229 may exert its renoprotective effects by preventing podocyte injury.

  10. DaVIE: Database for the Visualization and Integration of Epigenetic data

    PubMed Central

    Fejes, Anthony P.; Jones, Meaghan J.; Kobor, Michael S.

    2014-01-01

    One of the challenges in the analysis of large data sets, particularly in a population-based setting, is the ability to perform comparisons across projects. This has to be done in such a way that the integrity of each individual project is maintained, while ensuring that the data are comparable across projects. These issues are beginning to be observed in human DNA methylation studies, as the Illumina 450k platform and next generation sequencing-based assays grow in popularity and decrease in price. This increase in productivity is enabling new insights into epigenetics, but also requires the development of pipelines and software capable of handling the large volumes of data. The specific problems inherent in creating a platform for the storage, comparison, integration, and visualization of DNA methylation data include data storage, algorithm efficiency and ability to interpret the results to derive biological meaning from them. Databases provide a ready-made solution to these issues, but as yet no tools exist that that leverage these advantages while providing an intuitive user interface for interpreting results in a genomic context. We have addressed this void by integrating a database to store DNA methylation data with a web interface to query and visualize the database and a set of libraries for more complex analysis. The resulting platform is called DaVIE: Database for the Visualization and Integration of Epigenetics data. DaVIE can use data culled from a variety of sources, and the web interface includes the ability to group samples by sub-type, compare multiple projects and visualize genomic features in relation to sites of interest. We have used DaVIE to identify patterns of DNA methylation in specific projects and across different projects, identify outlier samples, and cross-check differentially methylated CpG sites identified in specific projects across large numbers of samples. A demonstration server has been setup using GEO data at http

  11. Modified Da Chengqi granules improvement in immune function in early severe acute pancreatitis patients.

    PubMed

    Jiang, D-L; Yang, J; Jiang, S-Y; Yuan, F-L; Gu, Y-L; Li, J-P; Pei, Z-J

    2016-01-01

    We investigated the role of modified Da Chengqi granules in improving immune function in early severe acute pancreatitis patients. Early severe acute pancreatitis patients who agreed to receive combined treatment of traditional Chinese and Western medicine were randomly assigned to the experimental or control group. All subjects received conventional therapy to support organ function. The experimental group also received modified Da Chengqi granules. Cytokine (interleukin-6, interleukin-10, and tumor necrosis factor-α) levels, immunological markers (HLA-DR, Treg, and Th1/Th2), urinary lactulose/mannitol ratio, and endotoxin levels were measured at 1, 3, 7, and 14 days after hospital admission. The total mortality rate was 11.69% (9/77), which was significantly lower in the experimental group [4.88% (2/41)] than in the control group [19.44% (7/36); χ(2) = 3.940, P < 0.05]. Serum interleukin-6, interleukin-10, tumor necrosis factor-α and endotoxin levels and the lactulose/mannitol ratio were significantly lower on day 7 and day 14 than on day 1 in experimental and control groups (P < 0.01). Immunological indices were significantly lower in the experimental group than in the control group on day 14 (all P < 0.01 or 0.05). HLA-DR-positive cell ratio gradually increased over 14 days in experimental and control groups (P < 0.01 vs day 1), but was higher in the experimental group than in the control group by day 14 (P < 0.05). Notably, Treg cell prevalence and Th1/Th2 cell ratio deteriorated within 7 days in both groups (P < 0.01 vs day 1), but then returned to day 1 levels (P < 0.01 or 0.05 vs day 1). Significant differences in Treg levels and Th1/Th2 cell ratio between experimental and control groups were observed on day 14 (P < 0.01). These results show that modified Da Chengqi granules can improve immune function in early severe acute pancreatitis patients.

  12. Prevalence of bands other than 160 and 130 kDa in pemphigus sera (a multicenter immunoblotting study). Gruppo Italiano Studi Epidemiologici in Dermatologia (GISED).

    PubMed

    Cozzani, E; Parodi, A; Rebora, A

    1998-05-01

    Patients with pemphigus may produce antibodies against molecules other than the classical transmembranal ones. Recently, for example antibodies to 230 kDa antigens have been found in association with antibodies to intercellular substance. To better understand their prevalence, clinical correlates and prognostic significance of bands other than 130 and 160 kDa, we studied 67 pemphigus sera. About one-fourth of patients revealed multiple heterogeneous bands and 13% the 230 kDa band. When challenged with the recombinant protein rBP55, the carbossiterminal portion of bullous pemphigoid major antigen, all 230 kDa-positive-sera proved negative. Caution is to be recommended in interpreting pemphigus sera with a band migrating at the 230 kDa level.

  13. Bundle Approach to Reduce Bloodstream Infections in Neutropenic Hematologic Patients with a Long-Term Central Venous Catheter.

    PubMed

    Martinez, Jose Manuel; Leite, Luís; França, Daniela; Capela, Rita; Viterbo, Luísa; Varajão, Natalina; Martins, Ângelo; Oliveira, Isabel; Domingues, Nélson; Moreira, Ilídia; Santo, Ana; Trigo, Filipe; Mariz, Jose

    2015-01-01

    Introdução: O objetivo deste estudo foi reduzir através de um pacote de medidas as infeções sistémicas e as taxas de infeções com origem no cateter venoso central nos doentes hematológicos em neutropenia com cateter venoso central de longa duração. Material e Métodos: Estudo prospetivo não randomizado realizado na unidade onco-hematológica do Instituto Português de Oncologia do Porto no período compreendido entre 1 de agosto de 2010 até 31 de janeiro de 2012. Durante este período foi introduzido um pacote de medidas (grupo estudo) e comparados os resultados nos 6 meses anteriores à sua implementação (grupo de controlo). As medidas consistiram na utilização de conectores de pressão neutra em detrimento dos conectores de pressão positiva, na sua troca mais frequente e numa solução anti-séptica mais eficaz. Foram incluídos neste estudo 116 doentes hematológicos com cateter venoso central de longa duração inserido por um período superior a 72 h. Foram contabilizados 8 867 dias de cateter (6 756 dias de cateter venoso central no grupo estudo e 2 111 dias de cateter venoso central no grupo de controlo). Resultados: Obteve-se uma redução significativa nas taxas de infeções sistémicas e infeções com origem no cateter venoso central. As taxas de infeções sistémicas: [32,69 (grupo de controlo) vs. 9,43 (grupo estudo)], com uma redução de incidência de 71% [risco relativo 0,2886, CI 95% (0,1793 - 0,4647), p < 0,001] e taxas de infeções com origem no cateter venoso central: [17,53 (grupo de controlo) vs. 4,73 (grupo estudo)], com redução de incidência de 71% [risco relativo 0,2936, CI 95% (0,1793 - 0,5615), p < 0,014]. Não foi encontrada diferença significativa (p > 0,05) na contagem de neutrófilos à data da colheita das amostras de hemoculturas entre ambos os grupos: 69% (< 500 neutrófilos/mm3) [71% (grupo estudo) vs. 68% (grupo de controlo)]. Conclusões: A introdução deste pacote de medidas baseado nas variáveis do

  14. Application of da Vinci(®) Robot in simple or radical hysterectomy: Tips and tricks.

    PubMed

    Iavazzo, Christos; Gkegkes, Ioannis D

    2016-01-01

    The first robotic simple hysterectomy was performed more than 10 years ago. These days, robotic-assisted hysterectomy is accepted as an alternative surgical approach and is applied both in benign and malignant surgical entities. The two important points that should be taken into account to optimize postoperative outcomes in the early period of a surgeon's training are how to achieve optimal oncological and functional results. Overcoming any technical challenge, as with any innovative surgical method, leads to an improved surgical operation timewise as well as for patients' safety. The standardization of the technique and recognition of critical anatomical landmarks are essential for optimal oncological and clinical outcomes on both simple and radical robotic-assisted hysterectomy. Based on our experience, our intention is to present user-friendly tips and tricks to optimize the application of a da Vinci® robot in simple or radical hysterectomies. PMID:27403078

  15. DA white dwarf effective temperatures determined from IUE Lyman-alpha profiles

    NASA Technical Reports Server (NTRS)

    Holberg, J. B.; Basile, J.; Wesemael, F.

    1986-01-01

    The Lyman-alpha profiles of 12 DA white dwarfs have been obtained with the International Ultraviolet Explorer Satellite. Analysis of these profiles provides an improved, uniform, and relatively bias-free measure of effective temperature for these stars over the range 20,000-60,000 K. Simultaneous estimates of surface gravity yield a mean gravity of log g = 7.96 for the entire sample, with the hottest stars tending to have the lowest gravities. A significant exception to this trend occurs in the case of the gravitation of HZ 43. An important by-product of this work has been the determination of a correction to IUE fluxes over the 1150-1350 A range.

  16. A 43 kDa recombinant plasmepsin elicits immune response in mice against Plasmodium berghei malaria.

    PubMed

    Pirta, Chhaya; Sharma, Nitya Nand; Banyal, H S

    2016-01-01

    Intraerythrocytic parasites degrade haemoglobin to make available nutrients for their growth and maturation. Plasmepsins, the aspartic proteases of Plasmodium play a significant role in haemoglobin degradation and are proposed as attractive drug targets. In the present study the gene which encodes plasmepsin in rodent malaria parasite, Plasmodium berghei, was cloned and expressed. The gene was sequenced and expressed in Escherichia coli BL21DE3 and a recombinant plasmepsin of molecular weight 43 kDa was obtained. The sequence obtained was analysed and compared with plasmepsins of other Plasmodium spp. Mice immunized with the recombinant plasmepsin induced a strong humoral immune response. ELISA and IFA performed on the serum of immunized mice showed high antibody titres. Along with this, in vivo study exhibited partial protection against P. berghei infection suggesting role of plasmepsin in malaria control.

  17. TSPO 18 kDa (PBR) Targeted Photosensitizers for Cancer Imaging (PET) and PDT.

    PubMed

    Chen, Yihui; Sajjad, Munawwar; Wang, Yanfang; Batt, Carrie; Nabi, Hani A; Pandey, Ravindra K

    2011-02-10

    Translocator protein (TSPO) 18 kDa overexpression has been observed in a large variety of human cancers, especially breast cancers. PK 11195, an isoquinoline analogue, is one of the ligands of highest TSPO binding affinity. Due to the long biological half life of our photosensitizers, there is a need to label them with a long lived radioisotope, for example I-124. Our objectives are to find translocator protein targeted photosensitizers for both tumor imaging (PET) and photodynamic therapy (PDT). I-PK 11195 is conjugated with the tumor avid photosensitizer HPPH. We find that those two tumor avid components complement each other and make the conjugate molecule even more tumor avid; compared to the photosensitizer itself, the conjugate is found to show improved PDT efficacy. It is concluded that I-PK 11195 can be a good vehicle to deliver radionuclide and photosensitizer to TSPO overexpressed tumor regions. Such conjugates could be useful for both tumor imaging (PET) and PDT.

  18. Basil as functional and preserving ingredient in "Serra da Estrela" cheese.

    PubMed

    Carocho, Márcio; Barros, Lillian; Barreira, João C M; Calhelha, Ricardo C; Soković, Marina; Fernández-Ruiz, Virginia; Buelga, Celestino Santos; Morales, Patricia; Ferreira, Isabel C F R

    2016-09-15

    Antitumor, antimicrobial and antioxidant activities of basil were studied, along with its characterization in phenolic compounds, organic acids and soluble sugars. The results placed basil as a valuable candidate for functionalization and conservation of food products, maintaining their nutritional properties, while increasing their shelf life and potential health effects. The basil leaves were then incorporated in "Serra da Estrela Cheese", either in its dehydrated form or as a decoction. The cheeses were then subject to a nutritional evaluation, being characterized for their fatty acids, minerals and CIE color parameters. To assess the combined effects of plant incorporation and storage time, a 2-way ANOVA was used to process the results, further analysed through a linear discriminant analysis. Overall, basil leaves provided antioxidant activity to the cheeses, reduced the moisture, and preserved the unsaturated fatty acids and proteins. Comparing both incorporation types, the decoctions had a higher functionalizing and conservative effect. PMID:27080879

  19. Application of da Vinci® Robot in simple or radical hysterectomy: Tips and tricks

    PubMed Central

    Iavazzo, Christos; Gkegkes, Ioannis D.

    2016-01-01

    The first robotic simple hysterectomy was performed more than 10 years ago. These days, robotic-assisted hysterectomy is accepted as an alternative surgical approach and is applied both in benign and malignant surgical entities. The two important points that should be taken into account to optimize postoperative outcomes in the early period of a surgeon’s training are how to achieve optimal oncological and functional results. Overcoming any technical challenge, as with any innovative surgical method, leads to an improved surgical operation timewise as well as for patients’ safety. The standardization of the technique and recognition of critical anatomical landmarks are essential for optimal oncological and clinical outcomes on both simple and radical robotic-assisted hysterectomy. Based on our experience, our intention is to present user-friendly tips and tricks to optimize the application of a da Vinci® robot in simple or radical hysterectomies. PMID:27403078

  20. Bell's palsy: the answer to the riddle of Leonardo da Vinci's 'Mona Lisa'.

    PubMed

    Maloney, W J

    2011-05-01

    The smile of the famed portrait 'The Mona Lisa' has perplexed both art historians and researchers for the past 500 years. There has been a multitude of theories expounded to explain the nature of the model's enigmatic smile. The origin of the model's wry smile can be demonstrated through a careful analysis of both documented facts concerning the portrait--some gathered only recently through the use of modern technology--and a knowledge of the clinical presentation of Bell's palsy. Bell's palsy is more prevalent in women who are either pregnant or who have recently given birth. This paper postulates that the smile of the portrait's model was due to Leonardo da Vinci's anatomically precise representation of a new mother affected by Bell's palsy subsequent to her recent pregnancy.

  1. Urodynamics in the anatomical work of Leonardo da Vinci (1452-1519).

    PubMed

    Schultheiss, D; Grünewald, V; Jonas, U

    1999-06-01

    Leonardo da Vinci (1452-1519) incorporates the symbiosis of art and medicine and can be addressed as the founder of medical illustration in the time of the Renaissance. His anatomy studies were not published in his time, which explains why Leonardo's outstanding knowledge of anatomy, physiology, and medicine had no impact on his scientific contemporaries and is therefore primarily of retrospective importance in the history of medicine. The collection of anatomical illustrations remained unknown until their rediscovery in the eighteenth century and their wide publication at the beginning of our century. This article systematically reviews Leonardo's genitourinary drawings with regard to urodynamic aspects of the upper and lower urinary tract, highlighting topics such as vesicoureteral reflux and urinary sphincter mechanisms.

  2. Middle Paleolithic human remains from the Gruta Da Oliveira (Torres Novas), Portugal.

    PubMed

    Willman, John C; Maki, Julia; Bayle, Priscilla; Trinkaus, Erik; Zilhão, João

    2012-09-01

    Additional Middle Paleolithic human remains from layers 17, 18, and 22 of the Gruta da Oliveira, Portugal consist of a proximal manual phalanx 2 (Oliveira 5), a partial postcanine tooth (Oliveira 6), a humeral diaphysis (Oliveira 7), a distal mandibular molar (Oliveira 8), and a mandibular premolar (P(3) ) (Oliveira 9). Oliveira 5, 6, and 8 are unremarkable for Late Pleistocene humans. The Oliveira 7 right humerus is moderately robust or the individual had the stocky body proportions of other European (including Iberian) Neandertals. The Oliveira 9 P(3) has a large and symmetrical crown and lacks a distal accessory ridge and accessory lingual cusps, overlapping both Neandertal and recent human ranges of variation. It contrasts with at least recent human P(3) s in having relatively thin enamel. These join the Oliveira 1 to 4 remains in further documenting early MIS 3 Neandertal morphology in western Iberia.

  3. A NEW TIMESCALE FOR PERIOD CHANGE IN THE PULSATING DA WHITE DWARF WD 0111+0018

    SciTech Connect

    Hermes, J. J.; Montgomery, M. H.; Winget, D. E.; Mullally, Fergal; Bischoff-Kim, A.

    2013-03-20

    We report the most rapid rate of period change measured to date for a pulsating DA (hydrogen atmosphere) white dwarf (WD), observed in the 292.9 s mode of WD 0111+0018. The observed period change, faster than 10{sup -12} s s{sup -1}, exceeds by more than two orders of magnitude the expected rate from cooling alone for this class of slow and simply evolving pulsating WDs. This result indicates the presence of an additional timescale for period evolution in these pulsating objects. We also measure the rates of period change of nonlinear combination frequencies and show that they share the evolutionary characteristics of their parent modes, confirming that these combination frequencies are not independent modes but rather artifacts of some nonlinear distortion in the outer layers of the star.

  4. THE KEPLER LIGHT CURVE OF THE UNIQUE DA WHITE DWARF BOKS 53856

    SciTech Connect

    Holberg, J. B.; Howell, Steve B. E-mail: howell@noao.edu

    2011-08-15

    The faint (g = 16.9) hot white dwarf BOKS 53856 was observed by the Kepler Mission in short cadence mode during mid-2009. Analysis of these observations reveals a highly stable modulation with a period of 6.1375 hr and a 2.46% half-amplitude. The folded light curve has an unusual shape that is difficult to explain in terms of a binary system containing an unseen companion more luminous than an L0 brown dwarf. Optical spectra of BOKS 53856 show a T{sub eff} = 34,000 K, log g = 8.0 DA white dwarf. There are few, if any, known white dwarfs in this temperature range exhibiting photometric variations similar to those we describe. A magnetic spin-modulated white dwarf model can in principle explain the light curve, an interpretation supported by spectral observations of the H{alpha} line showing evidence of Zeeman splitting.

  5. Basil as functional and preserving ingredient in "Serra da Estrela" cheese.

    PubMed

    Carocho, Márcio; Barros, Lillian; Barreira, João C M; Calhelha, Ricardo C; Soković, Marina; Fernández-Ruiz, Virginia; Buelga, Celestino Santos; Morales, Patricia; Ferreira, Isabel C F R

    2016-09-15

    Antitumor, antimicrobial and antioxidant activities of basil were studied, along with its characterization in phenolic compounds, organic acids and soluble sugars. The results placed basil as a valuable candidate for functionalization and conservation of food products, maintaining their nutritional properties, while increasing their shelf life and potential health effects. The basil leaves were then incorporated in "Serra da Estrela Cheese", either in its dehydrated form or as a decoction. The cheeses were then subject to a nutritional evaluation, being characterized for their fatty acids, minerals and CIE color parameters. To assess the combined effects of plant incorporation and storage time, a 2-way ANOVA was used to process the results, further analysed through a linear discriminant analysis. Overall, basil leaves provided antioxidant activity to the cheeses, reduced the moisture, and preserved the unsaturated fatty acids and proteins. Comparing both incorporation types, the decoctions had a higher functionalizing and conservative effect.

  6. Middle Paleolithic human remains from the Gruta Da Oliveira (Torres Novas), Portugal.

    PubMed

    Willman, John C; Maki, Julia; Bayle, Priscilla; Trinkaus, Erik; Zilhão, João

    2012-09-01

    Additional Middle Paleolithic human remains from layers 17, 18, and 22 of the Gruta da Oliveira, Portugal consist of a proximal manual phalanx 2 (Oliveira 5), a partial postcanine tooth (Oliveira 6), a humeral diaphysis (Oliveira 7), a distal mandibular molar (Oliveira 8), and a mandibular premolar (P(3) ) (Oliveira 9). Oliveira 5, 6, and 8 are unremarkable for Late Pleistocene humans. The Oliveira 7 right humerus is moderately robust or the individual had the stocky body proportions of other European (including Iberian) Neandertals. The Oliveira 9 P(3) has a large and symmetrical crown and lacks a distal accessory ridge and accessory lingual cusps, overlapping both Neandertal and recent human ranges of variation. It contrasts with at least recent human P(3) s in having relatively thin enamel. These join the Oliveira 1 to 4 remains in further documenting early MIS 3 Neandertal morphology in western Iberia. PMID:22610966

  7. First Results of the VLBI Experiment on Radar Location of the Asteroid 2012 DA14

    NASA Astrophysics Data System (ADS)

    Nechaeva, M.; Antipenko, A.; Bezrukov, D.; Bezrukovs, Vl.; Dementjev, A.; Dugin, N.; Jekabsons, N.; Khutornoy, R.; Klapers, M.; Konovalenko, A.; Kulishenko, V.; Nabatov, A.; Nesteruk, V.; Pupillo, G.; Reznichenko, A.; Salerno, E.; Shmeld, I.; Skirmante, K.; Tikhomirov, Yu.; Voytyuk, V.

    An international VLBI experiment on radio location of the asteroid 2012 DA14 was organized on 2013 February 15--16, during its flyby close to Earth. The purpose of observations was to investigate and specify orbital parameters of the asteroid, as well as to evaluate its rotation period and other characteristics. The irradiation of the asteroid was performed by the RT-70 transmitter at Evpatoria (Crimea, Ukraine), while the reflected signals were successfully accepted by the two 32 m radio telescopes at Medicina (Bologna, Italy) and Irbene (Ventspils, Latvia). Processing and interpretation of the data were performed both in the Radiophysical Research Institute and in the Ventspils International Radio Astronomy Center. The first results of this experiment are presented and discussed.

  8. Toward a Network of Faint DA White Dwarfs as High-precision Spectrophotometric Standards

    NASA Astrophysics Data System (ADS)

    Narayan, G.; Axelrod, T.; Holberg, J. B.; Matheson, T.; Saha, A.; Olszewski, E.; Claver, J.; Stubbs, C. W.; Bohlin, R. C.; Deustua, S.; Rest, A.

    2016-05-01

    We present the initial results from a program aimed at establishing a network of hot DA white dwarfs to serve as spectrophotometric standards for present and future wide-field surveys. These stars span the equatorial zone and are faint enough to be conveniently observed throughout the year with large-aperture telescopes. The spectra of these white dwarfs are analyzed in order to generate a non-local-thermodynamic-equilibrium model atmosphere normalized to Hubble Space Telescope colors, including adjustments for wavelength-dependent interstellar extinction. Once established, this standard star network will serve ground-based observatories in both hemispheres as well as space-based instrumentation from the UV to the near IR. We demonstrate the effectiveness of this concept and show how two different approaches to the problem using somewhat different assumptions produce equivalent results. We discuss the lessons learned and the resulting corrective actions applied to our program.

  9. Microbiological Analysis of Surfaces of Leonardo Da Vinci's Atlantic Codex: Biodeterioration Risk.

    PubMed

    Tarsitani, Gianfranco; Moroni, Catia; Cappitelli, Francesca; Pasquariello, Giovanna; Maggi, Oriana

    2014-01-01

    Following the discovery of discoloration on some pages of the Atlantic Codex (AC) of Leonardo da Vinci kept in the Biblioteca Ambrosiana in Milan, some investigations have been carried out to verify the presence of microorganisms, such as bacteria and fungi. To verify the presence of microorganisms a noninvasive method of sampling has been used that was efficient and allowed us to highlight the microbial facies of the material that was examined using conventional microbiological techniques. The microclimatic conditions in the storage room as well as the water content of the volume were also assessed. The combined observations allowed the conclusion that the discoloration of suspected biological origin on some pages of AC is not related to the presence or current attack of microbial agents.

  10. Microbiological Analysis of Surfaces of Leonardo Da Vinci's Atlantic Codex: Biodeterioration Risk

    PubMed Central

    Moroni, Catia; Pasquariello, Giovanna; Maggi, Oriana

    2014-01-01

    Following the discovery of discoloration on some pages of the Atlantic Codex (AC) of Leonardo da Vinci kept in the Biblioteca Ambrosiana in Milan, some investigations have been carried out to verify the presence of microorganisms, such as bacteria and fungi. To verify the presence of microorganisms a noninvasive method of sampling has been used that was efficient and allowed us to highlight the microbial facies of the material that was examined using conventional microbiological techniques. The microclimatic conditions in the storage room as well as the water content of the volume were also assessed. The combined observations allowed the conclusion that the discoloration of suspected biological origin on some pages of AC is not related to the presence or current attack of microbial agents. PMID:25574171

  11. [Teaching, research, and extension service: botanist Honório da Costa Monteiro Filho].

    PubMed

    Bovini, Massimo G; Peixoto, Ariane Luna

    2012-12-01

    The article revisits the work of Honório da Costa Monteiro Filho, highlighting his contribution to the study of economic botany and the taxonomy of Brazilian Malvaceae. Many of his seventy articles, are still cited. Yet little is known about his important role in educating agronomists involved with Brazilian flora and the creation of the Botanical Society of Brazil. These topics are discussed in the article, along with his work on a project to reform the teaching of agronomy in Brazil. The entire works of Monteiro Filho, archival documents, his correspondence with other scientists, and his observations on plant labels in herbaria were researched; interviews were also conducted with people with ties to him.

  12. Integrating Leonardo da Vinci's principles of demonstration, uncertainty, and cultivation in contemporary nursing education.

    PubMed

    Story, Lachel; Butts, Janie

    2014-03-01

    Nurses today are facing an ever changing health care system. Stimulated by health care reform and limited resources, nursing education is being challenged to prepare nurses for this uncertain environment. Looking to the past can offer possible solutions to the issues nursing education is confronting. Seven principles of da Vincian thinking have been identified (Gelb, 2004). As a follow-up to an exploration of the curiosità principle (Butts & Story, 2013), this article will explore the three principles of dimostrazione, sfumato, and corporalita. Nursing faculty can set the stage for a meaningful educational experience through these principles of demonstration (dimostrazione), uncertainty (sfumato), and cultivation (corporalita). Preparing nurses not only to manage but also to flourish in the current health care environment that will enhance the nurse's and patient's experience.

  13. The binary Feige 24 - The mass, radius, and gravitational redshift of the DA white dwarf

    NASA Technical Reports Server (NTRS)

    Vennes, Stephane; Shipman, Harry L.; Thorstensen, John R.; Thejll, Peter

    1991-01-01

    Observations are reported which refine the binary ephemeris of the Feige 24 system, which contains a peculiar hot DA white dwarf and an M dwarf with an atmosphere illuminated by extreme ultraviolet radiation from the white dwarf. With the new ephemeris and a set of IUE high-dispersion spectra, showing phase-dependent redshifted C IV, N V, and Si IV resonance lines, the orbital velocity, and hence the mass (0.54 + or - 0.20 solar masses), and the gravitational redshift of the white dwarf (14.1 + or - 5.2 km/s) are determined independently. It is shown that the measured Einstein redshift is consistent with an estimated radius for the white dwarf obtained from a model atmosphere solid angle and a parallax measurement. This radius is twice the Hamada-Salpeter radius for the given mass and offers a prospect to investigate the presence of a massive hydrogen envelope in that white dwarf star.

  14. Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants.

    PubMed

    Byun, Mi Young; Lee, Jungeun; Cui, Li Hua; Kang, Yoonjee; Oh, Tae Kyung; Park, Hyun; Lee, Hyoungseok; Kim, Woo Taek

    2015-07-01

    Deschampsia antarctica is an Antarctic hairgrass that grows on the west coast of the Antarctic peninsula. In this report, we have identified and characterized a transcription factor, D. antarctica C-repeat binding factor 7 (DaCBF7), that is a member of the monocot group V CBF homologs. The protein contains a single AP2 domain, a putative nuclear localization signal, and the typical CBF signature. DaCBF7, like other monocot group V homologs, contains a distinct polypeptide stretch composed of 43 amino acids in front of the AP2 motif. DaCBF7 was predominantly localized to nuclei and interacted with the C-repeat/dehydration responsive element (CRT/DRE) core sequence (ACCGAC) in vitro. DaCBF7 was induced by abiotic stresses, including drought, cold, and salinity. To investigate its possible cellular role in cold tolerance, a transgenic rice system was employed. DaCBF7-overexpressing transgenic rice plants (Ubi:DaCBF7) exhibited markedly increased tolerance to cold stress compared to wild-type plants without growth defects; however, overexpression of DaCBF7 exerted little effect on tolerance to drought or salt stress. Transcriptome analysis of a Ubi:DaCBF7 transgenic line revealed 13 genes that were up-regulated in DaCBF7-overexpressing plants compared to wild-type plants in the absence of cold stress and in short- or long-term cold stress. Five of these genes, dehydrin, remorin, Os03g63870, Os11g34790, and Os10g22630, contained putative CRT/DRE or low-temperature responsive elements in their promoter regions. These results suggest that overexpression of DaCBF7 directly and indirectly induces diverse genes in transgenic rice plants and confers enhanced tolerance to cold stress.

  15. The carboxy-terminal extension of the collagen binding domain of fibronectin mediates interaction with a 165 kDa membrane protein involved in odontoblast differentiation.

    PubMed

    Lesot, H; Fausser, J L; Akiyama, S K; Staub, A; Black, D; Kubler, M D; Ruch, J V

    1992-03-01

    Terminal differentiation of the odontoblast is characterized by an elongation and a polarization of the cell. The change in the cell shape and the reorganization of the cytoplasm involve the microfilament system. An immunological approach has previously implicated a transmembrane interaction between fibronectin and vinculin in the control of odontoblast differentiation. A 165 kDa protein localized on the cell-surface of odontoblasts mediated this interaction. In order to define the nature of the interaction of the 165 kDa protein with fibronectin, peptides were prepared by proteolytic cleavage of fibronectin with alpha-chymotrypsin. The results indicate that the 165 kDa protein interacted with a 62 kDa peptide located towards the amino-terminal extremity of fibronectin, but not with a 47 kDa related fragment. Both these 62 kDa and 47 kDa peptides included the collagen-binding domain and were retarded on a heparin-Ultrogel column. Microsequences demonstrated that the 62 kDa and 47 kDa fragments had the same amino-terminal extremity and that the larger fragment was extended in the carboxy-terminal direction. This carboxy-terminal extension of the collagen binding domain of fibronectin is implicated in the interaction of this molecule with the 165 kDa protein. On the other hand, odontoblasts differentiated normally when tooth germs were cultured in the presence of GRGDS synthetic peptide, suggesting that RGD-dependent integrins were not involved in odontoblast differentiation. Staining of dental mesenchymal cells in primary culture and of differentiated odontoblasts in situ with antibodies directed against the beta 1-subunit of integrins confirmed previous observations and showed that although beta 1 integrins are involved in the attachment of cultured dental cells, they are not implicated in the process of odontoblast differentiation. PMID:1597256

  16. 32 CFR Appendix D to Part 623 - Certificate for Signature by an Alternate (DA Form 4881-1-R)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Certificate for Signature by an Alternate (DA Form 4881-1-R) D Appendix D to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. D Appendix D to Part...

  17. Bringing the ZPD into the Equation: Capturing L2 Development during Computerized Dynamic Assessment (C-DA)

    ERIC Educational Resources Information Center

    Poehner, Matthew E.; Lantolf, James P.

    2013-01-01

    Dynamic Assessment (DA) derives from Vygotsky's (1987) insight that the use of mediation, attuned to learner needs, enables learners to perform beyond their current level of functioning, thereby providing insights into emerging capabilities. The instructional quality of mediation means that this process simultaneously creates possibilities…

  18. Allergenic Characterization of 27-kDa Glycoprotein, a Novel Heat Stable Allergen, from the Pupa of Silkworm, Bombyx mori

    PubMed Central

    Son, Mina; Lee, June Yong

    2016-01-01

    Boiled silkworm pupa is a traditional food in Asia, and patients with silkworm pupa food allergy are common in these regions. Still now only one allergen from silkworm, arginine kinase, has been identified. The purpose of this study was to identify novel food allergens in silkworm pupa by analyzing a protein extract after heat treatment. Heat treated extracts were examined by proteomic analysis. A 27-kDa glycoprotein was identified, expressed in Escherichia coli, and purified. IgE reactivity of the recombinant protein was investigated by ELISA. High molecular weight proteins (above 100 kDa) elicited increased IgE binding after heat treatment compared to that before heat treatment. The molecular identities of these proteins, however, could not be determined. IgE reactivity toward a 27-kDa glycoprotein was also increased after heating the protein extract. The recombinant protein was recognized by IgE antibodies from allergic subjects (33.3%). Glycation or aggregation of protein by heating may create new IgE binding epitopes. Heat stable allergens are shown to be important in silkworm allergy. Sensitization to the 27-kDa glycoprotein from silkworm may contribute to elevation of IgE to silkworm. PMID:26770033

  19. Molecular cloning and sequence of cDNA encoding polyoma medium tumor antigen-associated 61-kDa protein.

    PubMed Central

    Walter, G; Ferre, F; Espiritu, O; Carbone-Wiley, A

    1989-01-01

    Polyoma virus medium tumor antigen forms specific complexes with several cellular proteins; among these is a protein of approximately 61 kDa. With antibodies directed against medium tumor antigen, the 61-kDa protein was purified from human 293 cells that were infected with a hybrid adenovirus and overexpressed medium tumor antigen. The purified 61-kDa protein was partially digested with protease V8, and one of the protease V8 fragments was isolated and partially sequenced. The amino acid sequence information was used to design mixed oligonucleotide probes for screening a cDNA library from human placenta. A clone was isolated that hybridized with two separate probes; the clone contained an insert with an open reading frame for 589 amino acids. By in vitro translation of the transcript from this insert, a protein was generated that had the same size and yielded the same pattern of protease V8 fragments as the original 61-kDa protein. Its amino acid sequence reveals 15 repeats, the majority of which are 39 amino acids long. This protein bears no resemblance to proteins in the data bank that was searched. Images PMID:2554323

  20. 32 CFR Appendix D to Part 623 - Certificate for Signature by an Alternate (DA Form 4881-1-R)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Certificate for Signature by an Alternate (DA Form 4881-1-R) D Appendix D to Part 623 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY SUPPLIES AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. D Appendix D to Part...