40 CFR 180.1241 - Eucalyptus oil; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Eucalyptus oil; exemption from the... Exemptions From Tolerances § 180.1241 Eucalyptus oil; exemption from the requirement of a tolerance. Time-limited exemptions from the requirement of a tolerance are established for residues of eucalyptus oil on...

77 FR 33389 - ArborGen, LLC; Availability of an Environmental Assessment for Controlled Release of a...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-06

... trees derived from a hybrid of Eucalyptus grandis X Eucalyptus urophylla. The purpose of the field tests... growth; and to test the efficacy of genes designed to alter flowering. In addition, the trees have been... were introduced into Eucalyptus trees using disarmed Agrobacterium tumefaciens. The subject Eucalyptus...

40 CFR 180.1271 - Eucalyptus oil; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Eucalyptus oil; exemption from the... Exemptions From Tolerances § 180.1271 Eucalyptus oil; exemption from the requirement of a tolerance. An exemption from the requirement of tolerance is established for residues of eucalyptus oil in or on honey...

40 CFR 180.1241 - Eucalyptus oil; exemption from the requirement of a tolerance.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Eucalyptus oil; exemption from the... Exemptions From Tolerances § 180.1241 Eucalyptus oil; exemption from the requirement of a tolerance. Time-limited exemptions from the requirement of a tolerance are established for residues of eucalyptus oil on...

Physical and mechanical properties of saligna eucalyptus grown in Hawaii

Treesearch

C.C. Gerhards

1965-01-01

Physical and mechanical properties were determined for saligna eucalyptus (Eucalyptus saligna, Smith) grown in Hawaii. In comparison with wood of the same species grown in Australia, saligna eucalyptus grown in Hawaii was lower in density, shrinkage, and compressive strength parallel to grain; it was about equal in strength in bending and shear; and it was stiffer....

Eucalyptus Energy Farm: feasibility study and demonstration. Phase 1: site and species selection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mariani, E.O.; Wood, W.A.; Kouchoukos, P.C.

1978-06-01

The results of investigations to determine the feasibility for establishing ''Eucalyptus Energy Farms'' as a potential source of biomass derived energy are presented. Included in this report are: the results of an extensive literature search and numerous field interviews (both domestically and internationally) to identify those Eucalyptus species most suitable for use within the United States; a description of the site selection criteria, site selection process, and identification of potential sites for use in testing the Eucalyptus Energy Farm concept; a description of the Eucalyptus species selection criteria, species selection process, and an identification of the specific species recommended formore » testing at specific sites; preliminary engineering designs for each of the proposed plantation sites; and environmental impact considerations related to Eucalyptus growing. It is concluded that although the Eucalyptus are extremely temperature sensitive and the geographic areas which appear suitable for their planting (within the United States) are principally confined to western and central California and the warmest regions of the southeastern United States, each of the areas contain large amounts of marginal land which could be converted into Eucalyptus based biomass plantations. Suitable sites, which are representative of the larger marginal land areas, are immediately available for use in establishing Eucalyptus screening test sites. Furthermore, there are not significant environmental conditions which would preclude the establishment of these initial test sites. It is therefore recommended that efforts be initiated to obtain selected provenances of Eucalyptus seeds (of the designated species) for further field testing and data collection within the United States, and that actual site selection be initiated.« less

[Effects of introducing Eucalyptus on indigenous biodiversity].

PubMed

Ping, Liang; Xie, Zong-Qiang

2009-07-01

Eucalyptus is well-known as an effective reforestation tree species, due to its fast growth and high adaptability to various environments. However, the introduction of Eucalyptus could have negative effects on the local environment, e. g., inducing soil degradation, decline of groundwater level, and decrease of biodiversity, and especially, there still have controversies on the effects of introduced Eucalyptus on the understory biodiversity of indigenous plant communities and related mechanisms. Based on a detailed analysis of the literatures at home and abroad, it was considered that the indigenous plant species in the majority of introduced Eucalyptus plantations were lesser than those in natural forests and indigenous species plantations but more than those in other exotic species plantations, mainly due to the unique eco-physiological characteristics of Eucalyptus and the irrational plantation design and harvesting techniques, among which, anthropogenic factors played leading roles. Be that as it may, the negative effects of introducing Eucalyptus on local plant biodiversity could be minimized via more rigorous scientific plantation design and management based on local plant community characteristics. To mitigate the negative effects of Eucalyptus introduction, the native trees and understory vegetation in plantations should be kept intact during reforestation with Eucalyptus to favor the normal development of plant community and regeneration. At the same time, human disturbance should be minimized to facilitate the natural regeneration of native species.

Eucalyptus tolerance mechanisms to lanthanum and cerium: subcellular distribution, antioxidant system and thiol pools.

PubMed

Shen, Yichang; Zhang, Shirong; Li, Sen; Xu, Xiaoxun; Jia, Yongxia; Gong, Guoshu

2014-12-01

Guanglin 9 (Eucalyptus grandis × Eucalyptus urophlla) and Eucalyptus grandis 5 are two eucalyptus species which have been found to grow normally in soils contaminated with lanthanum and cerium, but the tolerance mechanisms are not clear yet. In this study, a pot experiment was conducted to investigate the tolerance mechanisms of the eucalyptus to lanthanum and cerium. Cell walls stored 45.40-63.44% of the metals under lanthanum or cerium stress. Peroxidase and catalase activities enhanced with increasing soil La or Ce concentrations up to 200 mg kg(-1), while there were no obvious changes in glutathione and ascorbate concentrations. Non-protein thiols concentrations increased with increasing treatment levels up to 200 mg kg(-1), and then decreased. Phytochelatins concentrations continued to increase under La or Ce stress. Therefore, the two eucalyptus species are La and Ce tolerant plants, and the tolerance mechanisms include cell wall deposition, antioxidant system response, and thiol compound synthesis. Copyright © 2014 Elsevier Ltd. All rights reserved.

Oldest Known Eucalyptus Macrofossils Are from South America

PubMed Central

Zamaloa, María C.; Nixon, Kevin C.; González, Cynthia C.; Wilf, Peter; Cúneo, N. Rubén; Johnson, Kirk R.

2011-01-01

The evolutionary history of Eucalyptus and the eucalypts, the larger clade of seven genera including Eucalyptus that today have a natural distribution almost exclusively in Australasia, is poorly documented from the fossil record. Little physical evidence exists bearing on the ancient geographical distributions or morphologies of plants within the clade. Herein, we introduce fossil material of Eucalyptus from the early Eocene (ca. 51.9 Ma) Laguna del Hunco paleoflora of Chubut Province, Argentina; specimens include multiple leaves, infructescences, and dispersed capsules, several flower buds, and a single flower. Morphological similarities that relate the fossils to extant eucalypts include leaf shape, venation, and epidermal oil glands; infructescence structure; valvate capsulate fruits; and operculate flower buds. The presence of a staminophore scar on the fruits links them to Eucalyptus, and the presence of a transverse scar on the flower buds indicates a relationship to Eucalyptus subgenus Symphyomyrtus. Phylogenetic analyses of morphological data alone and combined with aligned sequence data from a prior study including 16 extant eucalypts, one outgroup, and a terminal representing the fossils indicate that the fossils are nested within Eucalyptus. These are the only illustrated Eucalyptus fossils that are definitively Eocene in age, and the only conclusively identified extant or fossil eucalypts naturally occurring outside of Australasia and adjacent Mindanao. Thus, these fossils indicate that the evolution of the eucalypt group is not constrained to a single region. Moreover, they strengthen the taxonomic connections between the Laguna del Hunco paleoflora and extant subtropical and tropical Australasia, one of the three major ecologic-geographic elements of the Laguna del Hunco paleoflora. The age and affinities of the fossils also indicate that Eucalyptus subgenus Symphyomyrtus is older than previously supposed. Paleoecological data indicate that the Patagonian Eucalyptus dominated volcanically disturbed areas adjacent to standing rainforest surrounding an Eocene caldera lake. PMID:21738605

Eucalyptus globulus extract protects against UVB-induced photoaging by enhancing collagen synthesis via regulation of TGF-β/Smad signals and attenuation of AP-1.

PubMed

Park, Bom; Hwang, Eunson; Seo, Seul A; Cho, Jin-Gyeong; Yang, Jung-Eun; Yi, Tae-Hoo

2018-01-01

UV irradiation triggers the overproduction of matrix metalloproteinases and collagen degradation, which in turn causes increased pigmentation, dryness, and deep wrinkling of the skin. These chronic symptoms are collectively referred to as photoaging. Eucalyptus globulus is an evergreen tree that is widely used in cosmetics because of its antimicrobial activity. In this study, we investigated the protective effect of 50% ethanol extracts of Eucalyptus globulus on UV-induced photoaging in vitro and in vivo. Normal human dermal fibroblasts were treated with Eucalyptus globulus at concentrations ranging from 1 to 100 μg/mL after UVB or non-UVB irradiation. We found that Eucalyptus globulus suppressed the expression of MMPs and IL-6, but increased the expression of TGF-β1 and procollagen type 1. In addition, Eucalyptus globulus inhibited activation of the AP-1 transcription factor, an inducer of MMPs. Eucalyptus globulus was also found to regulate TGF-β/Smad signaling by reversing the activity of negative Smad regulators. Lastly, in vivo studies showed that topical application of Eucalyptus globulus on UVB-irradiated hairless mice reduced wrinkle formation and dryness by down-regulating MMP-1 and up-regulating expression of elastin, TGF-β1, and procollagen type 1. Taken together, these data suggest that Eucalyptus globulus may be a useful agent in cosmetic products. Copyright © 2017 Elsevier Inc. All rights reserved.

Control of Passion Fruit Fungal Diseases Using Essential Oils Extracted from Rosemary (Rosmarinus officinalis) and Eucalyptus (Eucalyptus agglomerata) in Egerton University Main Campus Njoro, Kenya

PubMed Central

Gathuru, Eliud Mugu; Githaiga, Benson Muriuki; Kimani, Salome Nduta

2017-01-01

Growth of fruits which form an important part of human diet has been jeopardized by the many fungal diseases that are present today. This study was conceived to isolate the most common fungal pathogens in passion fruits. Fungi were isolated using potato dextrose agar in addition to characterization using morphological, cultural, and biochemical means. Extraction of essential oils from rosemary (Rosmarinus officinalis) and eucalyptus (Eucalyptus agglomerata) was done. Before carrying the sensitivity test of essential oils to the fungal isolates, constituents of the essential oils were determined. The most common fungal pathogens isolated from passion fruits were Alternaria spp. (45%), Fusarium spp. (22%), Colletotrichum spp. (17%), and Penicillium spp. (16%). There was a relationship between heating time and yield of essential oils in rosemary (r = 0.99) and eucalyptus (r = 0.99). Conversely, there was no significant difference in the amount of essential oils produced by rosemary and eucalyptus (P = 0.08). Furthermore, there was a significant difference in growth inhibition of the fungal pathogens between essential oils from rosemary and eucalyptus (P = 0.000438). Fungal pathogens isolated from passion fruits can be controlled using essential oils from rosemary and eucalyptus. The oils need to be produced in large scale. PMID:28458692

Control of Passion Fruit Fungal Diseases Using Essential Oils Extracted from Rosemary (Rosmarinus officinalis) and Eucalyptus (Eucalyptus agglomerata) in Egerton University Main Campus Njoro, Kenya.

PubMed

Waithaka, Paul Njenga; Gathuru, Eliud Mugu; Githaiga, Benson Muriuki; Kimani, Salome Nduta

2017-01-01

Growth of fruits which form an important part of human diet has been jeopardized by the many fungal diseases that are present today. This study was conceived to isolate the most common fungal pathogens in passion fruits. Fungi were isolated using potato dextrose agar in addition to characterization using morphological, cultural, and biochemical means. Extraction of essential oils from rosemary ( Rosmarinus officinalis ) and eucalyptus ( Eucalyptus agglomerata ) was done. Before carrying the sensitivity test of essential oils to the fungal isolates, constituents of the essential oils were determined. The most common fungal pathogens isolated from passion fruits were Alternaria spp. (45%), Fusarium spp. (22%), Colletotrichum spp. (17%), and Penicillium spp. (16%). There was a relationship between heating time and yield of essential oils in rosemary ( r = 0.99) and eucalyptus ( r = 0.99). Conversely, there was no significant difference in the amount of essential oils produced by rosemary and eucalyptus ( P = 0.08). Furthermore, there was a significant difference in growth inhibition of the fungal pathogens between essential oils from rosemary and eucalyptus ( P = 0.000438). Fungal pathogens isolated from passion fruits can be controlled using essential oils from rosemary and eucalyptus. The oils need to be produced in large scale.

Pretreatment of Eucalyptus in biphasic system for furfural production and accelerated enzymatic hydrolysis.

PubMed

Zhang, Xiudong; Bai, Yuanyuan; Cao, Xuefei; Sun, Runcang

2017-08-01

Herein, an efficient biphasic pretreatment process was developed to improve the production of furfural (FF) and glucose from Eucalyptus. The influence of formic acid and NaCl on FF production from xylose in water and various biphasic systems was investigated. Results showed that the addition of formic acid and NaCl significantly promoted the FF yield, and the biphasic system of MIBK (methyl isobutyl ketone)/water exhibited the best performance for FF production. Then the Eucalyptus was pretreated in the MIBK/water system, and a maximum FF yield of 82.0% was achieved at 180°C for 60min. Surface of the pretreated Eucalyptus became relatively rough and loose, and its crystallinity index increased obviously due to the removal of hemicelluloses and lignin. The pretreated Eucalyptus samples showed much higher enzymatic hydrolysis rates (26.2-70.7%) than the raw Eucalyptus (14.5%). Copyright © 2017 Elsevier Ltd. All rights reserved.

Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight.

PubMed

Himaman, Winanda; Thamchaipenet, Arinthip; Pathom-Aree, Wasu; Duangmal, Kannika

2016-01-01

In Thailand, Eucalyptus plantations rapidly expand across the country. Leaf and shoot blight caused by Cryptosporiopsis eucalypti, Cylindrocladium sp. and Teratosphaeria destructans is a serious disease in Eucalyptus plantations. In this study, a total of 477 actinomycete strains were successfully isolated from roots and rhizosphere soil of Eucalyptus. Four hundred and thirty nine isolates were classified as streptomycetes and 38 isolates were non-streptomycetes. Among these isolates, 272 (57.0%), 118 (24.7%) and 241 (50.5%) isolates were antagonistic to Cryptosporiopsis eucalypti, Cylindrocladium sp. and Teratosphaeria destructans, respectively. All isolates were tested for their abilities to produce siderophores, indole acetic acid (IAA) and solubilise phosphate. Most isolates (464, 97.3%) produced siderophores. The majority of isolates (345, 72.3%) solubilised phosphate. In addition, almost half of these isolates (237, 49.7%) produced indole acetic acid. Strain EUSKR2S82 which showed the strongest inhibitory effect against all tested fungi with plant growth promoting ability was selected to test with Eucalyptus. This strain could colonize plant roots and increase Eucalyptus roots length. In a detached leaves bioassay, the disease severity of EUSKR2S82-inoculated Eucalyptus leaves was only 30% compared to 95% in the control treatment. The 16S rRNA gene sequence analysis revealed that the strain EUSKR2S82 was related to Streptomyces ramulosus NRRL-B 2714(T) (99.44% similarity). Identification of non-streptomycete isolates using 16S rRNA gene sequences classified them into 9 genera: Actinoallomurus, Actinomadura, Amycolatopsis, Cryptosporangium, Microbispora, Micromonospora, Nocardia, Nonomuraea and Pseudonocardia. It is evident that Eucalyptus tree harbored several genera of actinomycetes. The selected isolate, EUSKR2S82 showed potential as a candidate for biocontrol agent of leaf and shoot blight of Eucalyptus and to promote growth. Copyright © 2016 Elsevier GmbH. All rights reserved.

Eucalyptus Biodiesel as an Alternative to Diesel Fuel: Preparation and Tests on DI Diesel Engine

PubMed Central

Tarabet, Lyes; Loubar, Khaled; Lounici, Mohand Said; Hanchi, Samir; Tazerout, Mohand

2012-01-01

Nowadays, the increasing oil consumption throughout the world induces crucial economical, security, and environmental problems. As a result, intensive researches are undertaken to find appropriate substitution to fossil fuels. In view of the large amount of eucalyptus trees present in arid areas, we focus in this study on the investigation of using eucalyptus biodiesel as fuel in diesel engine. Eucalyptus oil is converted by transesterification into biodiesel. Eucalyptus biodiesel characterization shows that the physicochemical properties are comparable to those of diesel fuel. In the second phase, a single cylinder air-cooled, DI diesel engine was used to test neat eucalyptus biodiesel and its blends with diesel fuel in various ratios (75, 50, and 25 by v%) at several engine loads. The engine combustion parameters such as peak pressure, rate of pressure rise, and heat release rate are determined. Performances and exhaust emissions are also evaluated at all operating conditions. Results show that neat eucalyptus biodiesel and its blends present significant improvements of carbon monoxide, unburned hydrocarbon, and particulates emissions especially at high loads with equivalent performances to those of diesel fuel. However, the NOx emissions are slightly increased when the biodiesel content is increased in the blend. PMID:22675246

Eucalyptus biodiesel as an alternative to diesel fuel: preparation and tests on DI diesel engine.

PubMed

Tarabet, Lyes; Loubar, Khaled; Lounici, Mohand Said; Hanchi, Samir; Tazerout, Mohand

2012-01-01

Nowadays, the increasing oil consumption throughout the world induces crucial economical, security, and environmental problems. As a result, intensive researches are undertaken to find appropriate substitution to fossil fuels. In view of the large amount of eucalyptus trees present in arid areas, we focus in this study on the investigation of using eucalyptus biodiesel as fuel in diesel engine. Eucalyptus oil is converted by transesterification into biodiesel. Eucalyptus biodiesel characterization shows that the physicochemical properties are comparable to those of diesel fuel. In the second phase, a single cylinder air-cooled, DI diesel engine was used to test neat eucalyptus biodiesel and its blends with diesel fuel in various ratios (75, 50, and 25 by v%) at several engine loads. The engine combustion parameters such as peak pressure, rate of pressure rise, and heat release rate are determined. Performances and exhaust emissions are also evaluated at all operating conditions. Results show that neat eucalyptus biodiesel and its blends present significant improvements of carbon monoxide, unburned hydrocarbon, and particulates emissions especially at high loads with equivalent performances to those of diesel fuel. However, the NOx emissions are slightly increased when the biodiesel content is increased in the blend.

The tonoplast intrinsic aquaporin (TIP) subfamily of Eucalyptus grandis: Characterization of EgTIP2, a root-specific and osmotic stress-responsive gene.

PubMed

Rodrigues, Marcela I; Bravo, Juliana P; Sassaki, Flávio T; Severino, Fábio E; Maia, Ivan G

2013-12-01

Aquaporins have important roles in various physiological processes in plants, including growth, development and adaptation to stress. In this study, a gene encoding a root-specific tonoplast intrinsic aquaporin (TIP) from Eucalyptus grandis (named EgTIP2) was investigated. The root-specific expression of EgTIP2 was validated over a panel of five eucalyptus organ/tissues. In eucalyptus roots, EgTIP2 expression was significantly induced by osmotic stress imposed by PEG treatment. Histochemical analysis of transgenic tobacco lines (Nicotiana tabacum SR1) harboring an EgTIP2 promoter:GUS reporter cassette revealed major GUS staining in the vasculature and in root tips. Consistent with its osmotic-stress inducible expression in eucalyptus, EgTIP2 promoter activity was up-regulated by mannitol treatment, but was down-regulated by abscisic acid. Taken together, these results suggest that EgTIP2 might be involved in eucalyptus response to drought. Additional searches in the eucalyptus genome revealed the presence of four additional putative TIP coding genes, which could be individually assigned to the classical TIP1-5 groups. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Should Exotic Eucalyptus be Planted in Subtropical China: Insights from Understory Plant Diversity in Two Contrasting Eucalyptus Chronosequences.

PubMed

Wu, Jianping; Fan, Houbao; Liu, Wenfei; Huang, Guomin; Tang, Jianfu; Zeng, Ruijin; Huang, Jing; Liu, Zhanfeng

2015-11-01

Although Eucalyptus is widely planted in South China, whose effects on native biodiversity are unclear. The objective of this study was to quantify the richness and composition of understory plants in two contrasting Eucalyptus chronosequences in South China. One was in Zhangzhou City with plantation age of 2, 4, and 6 years after clear-cutting Chinese fir forests, while the other was in Heshan City with plantation age of 2, 3, and 24 years that reforested on barren lands. Results showed that the richness of understory plants and functional groups was not significantly altered in the Zhangzhou chronosequence, while increased in the 24-year-old plantations, with a significantly larger proportion of woody plants than the younger plantations for the Heshan chronosequence. Moreover, a higher richness of woody plants accompanied by a lower richness of herbaceous species was detected in the Zhangzhou chronosequence compared with the Heshan one. To balance the need for pulp production and plant diversity conservation, we suggest that intercropping approaches between exotic Eucalyptus plantations and native forests should be considered in the fast rotation Eucalyptus plantations. However, Eucalyptus plantations may be used as pioneer species to sustain ecosystem functioning for the degraded lands.

Soil microbial community structure and function responses to successive planting of Eucalyptus.

PubMed

Chen, Falin; Zheng, Hua; Zhang, Kai; Ouyang, Zhiyun; Li, Huailin; Wu, Bing; Shi, Qian

2013-10-01

Many studies have shown soil degradation after the conversion of native forests to exotic Eucalyptus plantations. However, few studies have investigated the long-term impacts of short-rotation forestry practices on soil microorganisms. The impacts of Eucalyptus successive rotations on soil microbial communities were evaluated by comparing phospholipid fatty acid (PLFA) abundances, compositions, and enzyme activities of native Pinus massoniana plantations and adjacent 1st, 2nd, 3rd, 4th generation Eucalyptus plantations. The conversion from P. massoniana to Eucalyptus plantations significantly decreased soil microbial community size and enzyme activities, and increased microbial physiological stress. However, the PLFA abundances formed "u" shaped quadratic functions with Eucalyptus plantation age. Alternatively, physiological stress biomarkers, the ratios of monounsaturated to saturated fatty acid and Gram+ to Gram- bacteria, formed "n"' shaped quadratic functions, and the ratio of cy17:0 to 16:1omega7c decreased with plantation age. The activities of phenol oxidase, peroxidase, and acid phosphatase increased with Eucalyptus plantation age, while the cellobiohydrolase activity formed "u" shaped quadratic functions. Soil N:P, alkaline hydrolytic nitrogen, soil organic carbon, and understory cover largely explained the variation in PLFA profiles while soil N:P, alkaline hydrolytic nitrogen, and understory cover explained most of the variability in enzyme activity. In conclusion, soil microbial structure and function under Eucalyptus plantations were strongly impacted by plantation age. Most of the changes could be explained by altered soil resource availability and understory cover associated with successive planting of Eucalyptus. Our results highlight the importance of plantation age for assessing the impacts of plantation conversion as well as the importance of reducing disturbance for plantation management.

Aggregate stability in soils cultivated with eucalyptus

USDA-ARS?s Scientific Manuscript database

Eucalyptus cultivation has increased in many Brazilian regions. In order to recommend good management practices, it is necessary to understand changes in soil properties where eucalyptus is planted. Aggregate stability analyses have proved to be a useful tool to measure soil effects caused by change...

A comparison of the effectiveness of chloroform and eucalyptus oil in dissolving root canal sealers.

PubMed

Schäfer, Edgar; Zandbiglari, Tannaz

2002-05-01

The solubility of 8 different root canal sealers in chloroform and in eucalyptus oil was compared. For standardized samples (n=12), ring molds were filled with mixed sealers based on epoxy resin, silicone, calcium hydroxide, zinc oxide-eugenol, glass ionomer, and polyketone. These samples were immersed in chloroform or eucalyptus oil for 30 seconds, 1 minute, 2 minutes, 5 minutes, 10 minutes, and 20 minutes. Then, the mean weight loss was determined and statistically analyzed. With the exception of the silicone, all the sealers showed significantly higher solubilities (P <.05) in chloroform than in eucalyptus oil. Epoxy resin was the most soluble sealer in chloroform. In eucalyptus oil, calcium hydroxide, and zinc oxide-eugenol showed the highest solubility. Under the conditions of this study, chloroform was a far more effective solvent of root canal sealers than eucalyptus oil. Because of the potential hazards of chloroform, further studies on the dissolution of root canal sealers in different solvents seem to be necessary.

Phytoremediation efficiency OF CD by Eucalyptus globulus transplanted from polluted and unpolluted sites.

PubMed

Luo, Jie; Qi, Shihua; Peng, Li; Wang, Jinji

2016-01-01

The capacity of plants to uptake heavy metals from contaminated soils has shown great phytoremediation potential. The development, resistibility and Cd extraction of Eucalyptus globulus individuals from metalliferous and clean sites in different years were analyzed under a specific environment. Eucalyptus globulus planted in Guiyu for phytoremediation or cultivated in an uncontaminated, natural environment for economic purposes were transplanted to Yuecheng town, which, in recent years, has been involved in the e-waste dismantling and recycling business, to compare the phytoremediation efficiency of Eucalyptus globulus trees grown in different environments. Trees cultivated in polluted areas can remove far more Cd and Hg from the contaminated soil than the individuals from clean soils because metalliferous Eucalyptus globulus can produce more biomass and uptake more heavy metals than nonmetalliferous plants per year. As polluted environments negatively affect the growth of plants, we speculated that the phytoremediation efficiency of metalliferous Eucalyptus globulus should decrease over time and that nonmetalliferous trees should adapt to the local environment.

Diversity and distribution of the endophytic bacterial community at different stages of Eucalyptus growth.

PubMed

Miguel, Paulo Sérgio Balbino; de Oliveira, Marcelo Nagem Valério; Delvaux, Júlio César; de Jesus, Guilherme Luiz; Borges, Arnaldo Chaer; Tótola, Marcos Rogério; Neves, Júlio César Lima; Costa, Maurício Dutra

2016-06-01

The relationships between plants and endophytic bacteria significantly contribute to plant health and yield. However, the microbial diversity in leaves of Eucalyptus spp. is still poorly characterized. Here, we investigated the endophytic diversity in leaves of hybrid Eucalyptus grandis x E. urophylla (Eucalyptus "urograndis") by using culture-independent and culture-dependent approaches, to better understand their ecology in leaves at different stages of Eucalyptus development, including bacteria with N2 fixation potential. Firmicutes, Proteobacteria (classes alpha-, beta- and gamma-) and Actinobacteria were identified in the Eucalyptus "urograndis" endophytic bacterial community. Within this community, the species Novosphingobium barchaimii, Rhizobium grahamii, Stenotrophomonas panacihumi, Paenibacillus terrigena, P. darwinianus and Terrabacter lapilli represent the first report these bacteria as endophytes. The diversity of the total endophytic bacteria was higher in the leaves from the 'field' (the Shannon-Wiener index, 2.99), followed by the indices obtained in the 'clonal garden' (2.78), the 'recently out from under shade (2.68), 'under shade' (2.63) and 'plants for dispatch' (2.51). In contrast, for diazotrophic bacteria, the highest means of these indices were obtained from the leaves of plants in the 'under shade' (2.56), 'recently out from under shade (2.52)' and 'field' stages (2.54). The distribution of the endophytic bacterial species in Eucalyptus was distinct and specific to the development stages under study, and many of the species had the potential for nitrogen fixation, raising the question of whether these bacteria could contribute to overall nitrogen metabolism of Eucalyptus.

Processed eucalyptus trees as a substrate component for greenhouse crop production

USDA-ARS?s Scientific Manuscript database

Fast growing eucalyptus species are selected for commercial plantings worldwide and are harvested for a variety of uses. Eucalyptus plantings in south Florida are harvested for landscape mulch production, yet this material may have potential as a container substrate for horticulture crop production....

Genetic basis of resistance in eucalyptus spp. pathosystems

Treesearch

Acelino Couto Alfenas; Lucio Mauro da Silva Guimaraes; Marcos Deon Vilela Resende

2012-01-01

Eucalyptus is the most widely planted hardwood crop in world-wide tropical and subtropical regions because of its high growth rate, broad adaptability, and multipurpose wood properties. Until the 1970s, the Eucalyptus plantations in Brazil were practically disease free. However, plantations have continued to expand into warmer...

Creating a database on Eucalyptus for California

Treesearch

Miles L. Merwin

1983-01-01

While public agencies, nurserymen and landscapers are receiving more inquiries about individual eucalyptus species for California plantings, little information is readily available to them on the potential usefulness or adaptability of these species. A survey of eucalyptus species was undertaken to collect information on their performance both in California trials,...

Eucalyptus beyond its native range: Environmental issues in exotic bioenergy plantations

Treesearch

John A. Stanturf; Eric D. Vance; Thomas R. Fox; Matias Kirst

2013-01-01

The genus Eucalyptus is native to Australia and Indonesia but has been widely planted in many countries. Eucalyptus has proven to be particularly successful in tropical and subtropical regions. Several species are also successful in some temperate regions, but problems with sudden and severe frosts pose limitations. Current...

Contenido de nutrientes en las raices finas y el mantillo de rodales de Eucalyptus grandis de diferente edad en la Mesopotomia Argentina [Fine roots and litter nutrient content of Eucalyptus grandis stands presenting different ages in Mesopotomia Argentina

Treesearch

C. Perez; J. Frangi; J.F. Goya; A. Luy; M. Arturi; NO-VALUE

2013-01-01

Entre Ríos province is an important center of Eucalyptus spp. plantations in Argentina. It was hypothesized that fine root biomass and litter mass increased with age increasing in plantations. Five, seven and seventeen year old stands of Eucalyptus grandis were sampled. All of them were first rotation stands. We estimated the mass of litter and fine roots (

76 FR 28118 - UBS AG., et al.; Notice of Application and Temporary Order

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-13

... Management, L.L.C. (``UBS Willow''), UBS Eucalyptus Management, L.L.C. (``UBS Eucalyptus'') and UBS Juniper Management, L.L.C. (``UBS Juniper'') (UBS Willow, UBS Eucalyptus, and UBS Juniper are referred to collectively as ``UBS Alternative Managers''); UBS Global Asset Management (Americas) Inc. (``UBS Global AM...

75 FR 2845 - ArborGen, LLC; Availability of an Environmental Assessment for Controlled Release of a...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-19

... Engineered Eucalyptus Hybrid AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Notice... for a proposed controlled field release of a genetically engineered clone of a Eucalyptus hybrid. This... proposed controlled field release of a genetically engineered clone of a Eucalyptus hybrid. \\1\\ To view the...

Projecting potential adoption of genetically engineered freeze-tolerant Eucalyptus in the United States

Treesearch

David N. Wear; Ernest Dixon IV; Robert C. Abt; Navinder Singh

2015-01-01

Development of commercial Eucalyptus plantations has been limited in the United States because of the species’ sensitivity to freezing temperatures. Recently developed genetically engineered clones of a Eucalyptus hybrid, which confer freeze tolerance, could expand the range of commercial plantations. This study explores how...

Herbicide site preparation and release options for eucalyptus plantation establishment in the western gulf

Treesearch

Michael A. Blazier; John Johnson; Eric L. Taylor; Brad Osbon

2012-01-01

Cold-tolerant species of eucalyptus (Eucalyptus spp.) are increasingly grown in the Western Gulf region as short-rotation pulpwood feedstock. Operational chemical suppression of competing vegetation has been relatively costly and inefficient because it requires frequent applications of glyphosate applied via backpack sprayers. A series of studies...

The use of polliniferous resources by Melipona capixaba, an endangered stingless bee species.

PubMed

Serra, Bruna Danielle Vieira; da Luz, Cynthia Fernandes Pinto; Campos, Lucio Antonio de Oliveira

2012-01-01

Abstract Pollen types present in samples from corbiculae of Melipona capixaba (Moure and Camargo) (Hymenoptera: Apidae: Meliponina) worker bees were analyzed, as well as pollen samples from food pots inside the hives in three sites located at the bees' original habitat. The aim was to find out the sources used as a trophic resource by this species. The dominant pollen grains in the spectrum of the samples belonged to the families Myrtaceae and Melastomataceae. Eucalyptus was the most frequent pollen type in the corbiculae in Conceição do Castelo municipality; Eucalyptus, Myrcia, and Melastomatacea/Combretaceae in the Fazenda do Estado district; and Eucalyptus and Myrcia in the São Paulo de Aracê district, both in the Domingos Martins municipality. Eucalyptus and Melastomataceae/Combretaceae were the predominant pollen types in the food pots. Eucalyptus was the most prevalent type all year round or most of the year. The most common pollen types in the months that Eucalyptus was not present or dominant in the samples were of remaining native forest species, "ruderal" (field) plants, fruit-bearing plants, and introduced ornamental plants.

The Use of Polliniferous Resources by Melipona capixaba, an Endangered Stingless Bee Species

PubMed Central

Serra, Bruna Danielle Vieira; da Luz, Cynthia Fernandes Pinto; Campos, Lucio Antonio de Oliveira

2012-01-01

Pollen types present in samples from corbiculae of Melipona capixaba (Moure and Camargo) (Hymenoptera: Apidae: Meliponina) worker bees were analyzed, as well as pollen samples from food pots inside the hives in three sites located at the bees’ original habitat. The aim was to find out the sources used as a trophic resource by this species. The dominant pollen grains in the spectrum of the samples belonged to the families Myrtaceae and Melastomataceae. Eucalyptus was the most frequent pollen type in the corbiculae in Conceição do Castelo municipality; Eucalyptus, Myrcia, and Melastomatacea/Combretaceae in the Fazenda do Estado district; and Eucalyptus and Myrcia in the São Paulo de Aracê district, both in the Domingos Martins municipality. Eucalyptus and Melastomataceae/Combretaceae were the predominant pollen types in the food pots. Eucalyptus was the most prevalent type all year round or most of the year. The most common pollen types in the months that Eucalyptus was not present or dominant in the samples were of remaining native forest species, “ruderal” (field) plants, fruit-bearing plants, and introduced ornamental plants. PMID:23464528

Pollen analysis of honey and pollen collected by Apis mellifera linnaeus, 1758 (Hymenoptera, Apidae), in a mixed environment of Eucalyptus plantation and native cerrado in Southeastern Brazil.

PubMed

Simeão, C M G; Silveira, F A; Sampaio, I B M; Bastos, E M A F

2015-11-01

Eucalyptus plantations are frequently used for the establishment of bee yards. This study was carried on at Fazenda Brejão, northwestern region of the State of Minas Gerais, Brazil. This farm is covered both with native Cerrado vegetation (Brazilian savanna) and eucalyptus plantations. This paper reports on the botanic origin of pollen pellets and honey collected from honeybee (Apis mellifera) hives along a thirteen-month period (January 2004 to January 2005). The most frequent pollen types found in the pollen pellets during the rainy season were Trema micrantha (Ulmaceae), Copaifera langsdorffii (Fabaceae), an unidentified Poaceae, unidentified Asteraceae-2, Cecropia sp. 1 (Cecropiaceae) and Eucalyptus spp. (Myrtaceae); during the dry season the most frequent pollen types were Acosmium dasycarpum (Fabaceae), Cecropia sp. 1 (Cecropiaceae) and Eucalyptus spp. (Myrtaceae). Pollen grains of Baccharis sp. (Asteraceae), Cecropia sp. 1 (Cecropiaceae), Copaifera langsdorffii (Fabaceae), Mimosa nuda (Fabaceae), Eucalyptus spp. (Myrtaceae) and Trema micrantha (Ulmaceae) were present in the honey samples throughout the study period.

Eucalyptus applied genomics: from gene sequences to breeding tools.

PubMed

Grattapaglia, Dario; Kirst, Matias

2008-01-01

Eucalyptus is the most widely planted hardwood crop in the tropical and subtropical world because of its superior growth, broad adaptability and multipurpose wood properties. Plantation forestry of Eucalyptus supplies high-quality woody biomass for several industrial applications while reducing the pressure on tropical forests and associated biodiversity. This review links current eucalypt breeding practices with existing and emerging genomic tools. A brief discussion provides a background to modern eucalypt breeding together with some current applications of molecular markers in support of operational breeding. Quantitative trait locus (QTL) mapping and genetical genomics are reviewed and an in-depth perspective is provided on the power of association genetics to dissect quantitative variation in this highly diverse organism. Finally, some challenges and opportunities to integrate genomic information into directional selective breeding are discussed in light of the upcoming draft of the Eucalyptus grandis genome. Given the extraordinary genetic variation that exists in the genus Eucalyptus, the ingenuity of most breeders, and the powerful genomic tools that have become available, the prospects of applied genomics in Eucalyptus forest production are encouraging.

Eucalyptus essential oil as a natural food preservative: in vivo and in vitro antiyeast potential.

PubMed

Tyagi, Amit Kumar; Bukvicki, Danka; Gottardi, Davide; Tabanelli, Giulia; Montanari, Chiara; Malik, Anushree; Guerzoni, Maria Elisabetta

2014-01-01

In this study, the application of eucalyptus essential oil/vapour as beverages preservative is reported. The chemical composition of eucalyptus oil was determined by gas chromatography-mass spectrometry (GC-MS) and solid phase microextraction GC-MS (SPME/GC-MS) analyses. GC-MS revealed that the major constituents were 1,8-cineole (80.5%), limonene (6.5%), α-pinene (5%), and γ-terpinene (2.9%) while SPME/GC-MS showed a relative reduction of 1,8-cineole (63.9%) and an increase of limonene (13.8%), α-pinene (8.87%), and γ-terpinene (3.98%). Antimicrobial potential of essential oil was initially determined in vitro against 8 different food spoilage yeasts by disc diffusion, disc volatilization, and microdilution method. The activity of eucalyptus vapours was significantly higher than the eucalyptus oil. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) varied from 0.56 to 4.50 mg/mL and from 1.13 to 9 mg/mL, respectively. Subsequently, the combined efficacy of essential oil and thermal treatment were used to evaluate the preservation of a mixed fruit juice in a time-dependent manner. These results suggest eucalyptus oil as a potent inhibitor of food spoilage yeasts not only in vitro but also in a real food system. Currently, this is the first report that uses eucalyptus essential oil for fruit juice preservation against food spoiling yeast.

Eucalyptus Essential Oil as a Natural Food Preservative: In Vivo and In Vitro Antiyeast Potential

PubMed Central

Bukvicki, Danka; Gottardi, Davide; Malik, Anushree; Guerzoni, Maria Elisabetta

2014-01-01

In this study, the application of eucalyptus essential oil/vapour as beverages preservative is reported. The chemical composition of eucalyptus oil was determined by gas chromatography-mass spectrometry (GC-MS) and solid phase microextraction GC-MS (SPME/GC-MS) analyses. GC-MS revealed that the major constituents were 1,8-cineole (80.5%), limonene (6.5%), α-pinene (5%), and γ-terpinene (2.9%) while SPME/GC-MS showed a relative reduction of 1,8-cineole (63.9%) and an increase of limonene (13.8%), α-pinene (8.87%), and γ-terpinene (3.98%). Antimicrobial potential of essential oil was initially determined in vitro against 8 different food spoilage yeasts by disc diffusion, disc volatilization, and microdilution method. The activity of eucalyptus vapours was significantly higher than the eucalyptus oil. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) varied from 0.56 to 4.50 mg/mL and from 1.13 to 9 mg/mL, respectively. Subsequently, the combined efficacy of essential oil and thermal treatment were used to evaluate the preservation of a mixed fruit juice in a time-dependent manner. These results suggest eucalyptus oil as a potent inhibitor of food spoilage yeasts not only in vitro but also in a real food system. Currently, this is the first report that uses eucalyptus essential oil for fruit juice preservation against food spoiling yeast. PMID:25177704

Influence of nutrient availability, stand age, and canopy structure on isoprene flux in a Eucalyptus saligna experimental forest

Treesearch

Jennifer L. Funk; Christian P. Giardina; Alexander Knohl; Manuel T. Lerdau

2006-01-01

Eucalyptus plantations occupy approximately 10 million ha of land in the tropics and, increasingly, afforestation and reforestation projects are relying on this genus to provide rapid occupation of degraded sites, large quantities of high-quality wood products, and high rates of carbon sequestration. Members of the genus Eucalyptus...

Potential implications for expansion of freeze-tolerant eucalyptus plantations on water resources in the southern United States

Treesearch

James M. Vose; Chelcy F. Miniat; Ge Sun; Peter V. Caldwell

2014-01-01

The potential expansion of freeze-tolerant (FT) Eucalyptus plantations in the United States has raised concerns about the implications for water resources. Modeling was used to examine the potential effects of expanding the distribution of FT Eucalyptus plantations in US Department of Agriculture Plant Hardiness Zones 8b and...

Performance of Australian provenances of Eucalyptus grandis and Eucalyptus saligna in Hawaii

Treesearch

Roger G. Skolmen

1986-01-01

Australian provenances of Eucalyptus grandis and E. saligna were compared at four locations on the island of Hawaii to seek seed sources better than those in current use which were introduced earlier from unrecorded locations in Australia. A broad range of latitude and elevation was represented among the provenances. At all four...

Eucalyptus gall wasp, Leptocybe invasa Fisher & La Salle (Insecta: Hymenoptera: Eulophidae), an emerging pest of eucalyptus in Florida

USDA-ARS?s Scientific Manuscript database

A new emerging pest of eucalyptus, Leptocybe invasa Fisher & La Salle, was first found in Italy but mistakenly identified as Aprostocetus sp.. This was followed by another report of an infestation from Turkey in early 2000. It was first formally described in 2004 from Australia as Leptocybe invasa a...

Methanol production from Eucalyptus wood chips. Working document I. The Florida Eucalyptus energy farm: silvicultural methods and considerations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fishkind, H.H.

The silvicultural matrix within which the nation's first large scale wood energy plantation will develop is described in detail. The relevant literature reviewed is identified and distilled. The plantation history, site preparation, planting, species selection, maintenance and management, harvesting, and the Eucalyptus biomass production estimates are presented.

Effect of surfactant concentration on the spreading properties of pesticide droplets on Eucalyptus leaves

USDA-ARS?s Scientific Manuscript database

The area wetted by 500-µm diameter droplets of pesticide and deionised water placed at different positions on Eucalyptus urophylla × E. grandis (E.u × E.g) and Eucalyptus tereticornis (E.t) leaves was determined at an air temperature of 30 °C and a relative humidity of 60%. Dimethyl dichlorovinyl ph...

A novel genome-wide microsatellite resource for species of Eucalyptus with linkage-to-physical correspondence on the reference genome sequence.

PubMed

Grattapaglia, Dario; Mamani, Eva M C; Silva-Junior, Orzenil B; Faria, Danielle A

2015-03-01

Keystone species in their native ranges, eucalypts, are ecologically and genetically very diverse, growing naturally along extensive latitudinal and altitudinal ranges and variable environments. Besides their ecological importance, eucalypts are also the most widely planted trees for sustainable forestry in the world. We report the development of a novel collection of 535 microsatellites for species of Eucalyptus, 494 designed from ESTs and 41 from genomic libraries. A selected subset of 223 was evaluated for individual identification, parentage testing, and ancestral information content in the two most extensively studied species, Eucalyptus grandis and Eucalyptus globulus. Microsatellites showed high transferability and overlapping allele size range, suggesting they have arisen still in their common ancestor and confirming the extensive genome conservation between these two species. A consensus linkage map with 437 microsatellites, the most comprehensive microsatellite-only genetic map for Eucalyptus, was built by assembling segregation data from three mapping populations and anchored to the Eucalyptus genome. An overall colinearity between recombination-based and physical positioning of 84% of the mapped microsatellites was observed, with some ordering discrepancies and sporadic locus duplications, consistent with the recently described whole genome duplication events in Eucalyptus. The linkage map covered 95.2% of the 605.8-Mbp assembled genome sequence, placing one microsatellite every 1.55 Mbp on average, and an overall estimate of physical to recombination distance of 618 kbp/cM. The genetic parameters estimates together with linkage and physical position data for this large set of microsatellites should assist marker choice for genome-wide population genetics and comparative mapping in Eucalyptus. © 2014 John Wiley & Sons Ltd.

Response of Groundwater table to Eucalyptus Plantations in a Tropical Monsoon Climate, Lake Tana Basin, Ethiopia

NASA Astrophysics Data System (ADS)

Enku, Temesgen; Melesse, Assefa; Ayana, Essaya; Tilahun, Seifu; Abate, Mengiste; Steenhuis, Tammo

2017-04-01

Given the increasing demand for water resources and the need for better management of regional water resources, it is essential to quantify the groundwater use by phreatophytes in tropical monsoon climates. Phreatophytes, like eucalyptus plantations are reported to be a groundwater sink and it could significantly affect the regional groundwater resources. In our study, the consumptive groundwater use of a closed eucalyptus plantation was calculated based on the diurnal water table fluctuations observed in monitoring wells for two dry monsoon phases in the Fogera plain, northwest of Ethiopia. Automated recorders were installed to monitor the hourly groundwater table fluctuations. The groundwater table fluctuates from maximum at early in the morning to minimum in the evening daily and generally declined linearly during the dry phase averaging 3.1 cm/day during the two year period under the eucalyptus plantations. The hourly eucalypts transpiration rate over the daylight hours follows the daily solar irradiance curve for clear sky days. It is minimal during the night and reaches maximum of 1.65mm/hour at mid-day. The evapotranspiration from the groundwater by eucalyptus plantations during the dry phases was estimated at about 2300mm from October 1 to 31 May, in 2015 compared to about 900mm without eucalyptus trees. The average daily evapotranspiration was 9.6mm. This is almost twice of the reference evapotranspiration in the area and 2.5 times the actual rate under fallow agricultural fields. Thus, water resources planning and management in the region needs to consider the effect of eucalyptus plantations on the availability of groundwater resources in the highlands of Ethiopia. Key words: Eucalyptus, Evapotranspiration, Groundwater, Ethiopia, Lake Tana

Methanol production from Eucalyptus wood chips. Working Document 9. Economics of producing methanol from Eucalyptus in Central Florida

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fishkind, H.H.

1982-06-01

A detailed feasibility study of producing methanol from Eucalyptus in Central Florida encompasses all phases of production - from seedling to delivery of finished methanol. The project includes the following components: (1) production of 55 million, high quality, Eucalyptus seedlings through tissue culture; (2) establishment of a Eucalyptus energy plantation on approximately 70,000 acres; and (3) engineering for a 100 million gallon-per-year methanol production facility. In addition, the potential environmental impacts of the whole project were examined, safety and health aspects of producing and using methanol were analyzed, and site specific cost estimates were made. The economics of the projectmore » are presented here. Each of the three major components of the project - tissue culture lab, energy plantation, and methanol refinery - are examined individually. In each case a site specific analysis of the potential return on investment was conducted.« less

Short-rotation management of Eucalyptus: Guidelines for plantations in Hawaii. Forest Service general technical report (Final)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Whitesell, C.D.; DeBell, D.S.; Schubert, T.H.

1992-11-01

A 10-year research and development program was conducted on the island of Hawaii, where nearly 230,000 acres are suitable for growing biomass in short-rotation Eucalyptus plantations. Successful techniques are described for seedling production, plantation establishment (site preparation, weed control, planting), maintenance (weed control, fertilization), biomass yield estimation, and harvest. Basic biological relationships are described to aid decisions on site selection, initial spacing, fertilizer schedules, and rotation length. Environmental issues likely to be faced by growers of Eucalyptus plantations are discussed, including soil erosion, nutrient depletion, and monocultures. Continuing programs for tree improvement, monitoring, and silviculture research are recommeded. Production costsmore » for biomass yields are estimated for three promising management regimes, representing pure Eucalyptus plantings at dense and wide spacings and a mixed species plantation where Albizia is used as a nurse crop to provide nitrogen needed for optimum Eucalyptus growth.« less

78 FR 13309 - ArborGen Inc.; Availability of Petition, Notice of Intent To Prepare an Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-27

... incorporation of the GE trait allows these eucalyptus hybrid trees to be grown in a broader geographic area than non-GE eucalyptus hybrid trees. The petition has been submitted in accordance with our regulations... designated 427 and 435. The petition states that these eucalyptus trees are unlikely to pose a plant pest...

Modelo empirico integral de una plantacion de Eucalyptus grandis en Concordia, Entre Rios

Treesearch

Jorge Frangi; Carolina Perez; Juan Goya; Natalia Teson; Marcelo Barrera; Marcelo Arturi

2016-01-01

The Argentinian Mesopotamia is the core of fast-growing tree species plantations of the country. Eucalyptus grandis plantations constitute 90 % of the forested area with Eucalyptus spp. in NE Entre Rios. Based on previous studies on structural and functional features, a comprehensive model is here proposed on emergence of new properties linked to matter and ecosystem...

Repellent activity of five essential oils against Culex pipiens.

PubMed

Erler, F; Ulug, I; Yalcinkaya, B

2006-12-01

Essential oils extracted from the seeds of anise (Pimpinella anisum), dried fruits of eucalyptus (Eucalyptus camaldulensis), dried foliage of mint (Mentha piperita) and basil (Ocimum basilicum) and fresh foliage of laurel (Laurus nobilis) were tested for their repellency against the adult females of Culex pipiens. All essential oils showed repellency in varying degrees, eucalyptus, basil and anise being the most active.

Variation in natural durability of seven Eucalyptus grandis x Eucalyptus urophylla hybrid clones

Treesearch

F.J.N. Franca; T.S.F.A. Franca; R.A Arango; B.M. Woodward; G.B. Vidaurre

2017-01-01

Programs aimed at developing clones of hybrid trees are commonly established in Brazil to meet the demands of various forest-based industries. These programs have continually improved the quality of eucalyptus wood, which has the potential to reduce deforestation by lowering demand for other high-value species. This is particularly true in the lumber market, but little...

Effects of irrigation on water use and water use efficiency in two fast growing Eucalyptus plantations

Treesearch

Robert M. Hubbard; Jose Stape; Michael G. Ryan; Auro C. Almeida; Juan Rojas

2010-01-01

Eucalyptus plantations occupy almost 20 million ha worldwide and exceed 3.7 million ha in Brazil alone. Improved genetics and silviculture have led to as much as a three-fold increase in productivity in Eucalyptus plantations in Brazil and the large land area occupied by these highly productive ecosystems raises concern over their...

Encapsulated eucalyptus oil in ionically cross-linked alginate microcapsules and its controlled release.

PubMed

Noppakundilograt, Supaporn; Piboon, Phianghathai; Graisuwan, Wilaiporn; Nuisin, Roongkan; Kiatkamjornwong, Suda

2015-10-20

Sodium alginate microcapsules containing eucalyptus oil were prepared by oil-in-water emulsification via Shirasu porous glass (SPG) membrane and cross-linked by calcium chloride (CaCl2). SPG membrane pore size of 5.2μm was used to control the size of eucalyptus oil microdroplets. Effects of sodium alginate, having a mannuronic acid/guluronic acid (M/G) ratio of 1.13, eucalyptus oil and CaCl2 amounts on microdroplet sizes and size distribution were elucidated. Increasing sodium alginate amounts from 0.1 to 0.5% (wv(-1)) sodium alginate, the average droplets size increased from 42.2±2.0 to 48.5±0.6μm, with CVs of 16.5±2.2 and 30.2±4.5%, respectively. CaCl2 successfully gave narrower size distribution of cross-linked eucalyptus oil microcapsules. The optimum conditions for preparing the microcapsules, oil loading efficiency, and controlled release of the encapsulated eucalyptus oil from the microcapsules as a function of time at 40°C were investigated. Release model for the oil from microcapsules fitted Ritger-Peppas model with non-Fickian transport mechanism. Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical Variability and Biological Activities of Eucalyptus spp. Essential Oils.

PubMed

Barbosa, Luiz Claudio Almeida; Filomeno, Claudinei Andrade; Teixeira, Robson Ricardo

2016-12-07

Many plant species produce mixtures of odorous and volatile compounds known as essential oils (EOs). These mixtures play important roles in Nature and have been utilized by mankind for different purposes, such as pharmaceuticals, agrochemicals, aromatherapy, and food flavorants. There are more than 3000 EOs reported in the literature, with approximately 300 in commercial use, including the EOs from Eucalyptus species. Most EOs from Eucalyptus species are rich in monoterpenes and many have found applications in pharmaceuticals, agrochemicals, food flavorants, and perfumes. Such applications are related to their diverse biological and organoleptic properties. In this study, we review the latest information concerning the chemical composition and biological activities of EOs from different species of Eucalyptus . Among the 900 species and subspecies of the Eucalyptus genus, we examined 68 species. The studies associated with these species were conducted in 27 countries. We have focused on the antimicrobial, acaricidal, insecticidal and herbicidal activities, hoping that such information will contribute to the development of research in this field. It is also intended that the information described in this study can be useful in the rationalization of the use of Eucalyptus EOs as components for pharmaceutical and agrochemical applications as well as food preservatives and flavorants.

Fungal Planet description sheets: 469-557.

PubMed

Crous, P W; Wingfield, M J; Burgess, T I; Hardy, G E St J; Crane, C; Barrett, S; Cano-Lira, J F; Le Roux, J J; Thangavel, R; Guarro, J; Stchigel, A M; Martín, M P; Alfredo, D S; Barber, P A; Barreto, R W; Baseia, I G; Cano-Canals, J; Cheewangkoon, R; Ferreira, R J; Gené, J; Lechat, C; Moreno, G; Roets, F; Shivas, R G; Sousa, J O; Tan, Y P; Wiederhold, N P; Abell, S E; Accioly, T; Albizu, J L; Alves, J L; Antoniolli, Z I; Aplin, N; Araújo, J; Arzanlou, M; Bezerra, J D P; Bouchara, J-P; Carlavilla, J R; Castillo, A; Castroagudín, V L; Ceresini, P C; Claridge, G F; Coelho, G; Coimbra, V R M; Costa, L A; da Cunha, K C; da Silva, S S; Daniel, R; de Beer, Z W; Dueñas, M; Edwards, J; Enwistle, P; Fiuza, P O; Fournier, J; García, D; Gibertoni, T B; Giraud, S; Guevara-Suarez, M; Gusmão, L F P; Haituk, S; Heykoop, M; Hirooka, Y; Hofmann, T A; Houbraken, J; Hughes, D P; Kautmanová, I; Koppel, O; Koukol, O; Larsson, E; Latha, K P D; Lee, D H; Lisboa, D O; Lisboa, W S; López-Villalba, Á; Maciel, J L N; Manimohan, P; Manjón, J L; Marincowitz, S; Marney, T S; Meijer, M; Miller, A N; Olariaga, I; Paiva, L M; Piepenbring, M; Poveda-Molero, J C; Raj, K N A; Raja, H A; Rougeron, A; Salcedo, I; Samadi, R; Santos, T A B; Scarlett, K; Seifert, K A; Shuttleworth, L A; Silva, G A; Silva, M; Siqueira, J P Z; Souza-Motta, C M; Stephenson, S L; Sutton, D A; Tamakeaw, N; Telleria, M T; Valenzuela-Lopez, N; Viljoen, A; Visagie, C M; Vizzini, A; Wartchow, F; Wingfield, B D; Yurchenko, E; Zamora, J C; Groenewald, J Z

2016-12-01

Novel species of fungi described in this study include those from various countries as follows: Australia : Apiognomonia lasiopetali on Lasiopetalum sp., Blastacervulus eucalyptorum on Eucalyptus adesmophloia , Bullanockia australis (incl. Bullanockia gen. nov.) on Kingia australis , Caliciopsis eucalypti on Eucalyptus marginata , Celerioriella petrophiles on Petrophile teretifolia , Coleophoma xanthosiae on Xanthosia rotundifolia , Coniothyrium hakeae on Hakea sp., Diatrypella banksiae on Banksia formosa , Disculoides corymbiae on Corymbia calophylla , Elsinoë eelemani on Melaleuca alternifolia , Elsinoë eucalyptigena on Eucalyptus kingsmillii , Elsinoë preissianae on Eucalyptus preissiana , Eucasphaeria rustici on Eucalyptus creta , Hyweljonesia queenslandica (incl. Hyweljonesia gen. nov.) on the cocoon of an unidentified microlepidoptera, Mycodiella eucalypti (incl. Mycodiella gen. nov.) on Eucalyptus diversicolor , Myrtapenidiella sporadicae on Eucalyptus sporadica , Neocrinula xanthorrhoeae (incl. Neocrinula gen. nov.) on Xanthorrhoea sp., Ophiocordyceps nooreniae on dead ant, Phaeosphaeriopsis agavacearum on Agave sp., Phlogicylindrium mokarei on Eucalyptus sp., Phyllosticta acaciigena on Acacia suaveolens , Pleurophoma acaciae on Acacia glaucoptera , Pyrenochaeta hakeae on Hakea sp., Readeriella lehmannii on Eucalyptus lehmannii , Saccharata banksiae on Banksia grandis , Saccharata daviesiae on Daviesia pachyphylla , Saccharata eucalyptorum on Eucalyptus bigalerita , Saccharata hakeae on Hakea baxteri , Saccharata hakeicola on Hakea victoria , Saccharata lambertiae on Lambertia ericifolia , Saccharata petrophiles on Petrophile sp., Saccharata petrophilicola on Petrophile fastigiata , Sphaerellopsis hakeae on Hakea sp., and Teichospora kingiae on Kingia australis. Brazil : Adautomilanezia caesalpiniae (incl . Adautomilanezia gen. nov.) on Caesalpina echinata , Arthrophiala arthrospora (incl. Arthrophiala gen. nov.) on Sagittaria montevidensis , Diaporthe caatingaensis (endophyte from Tacinga inamoena ), Geastrum ishikawae on sandy soil, Geastrum pusillipilosum on soil, Gymnopus pygmaeus on dead leaves and sticks, Inonotus hymenonitens on decayed angiosperm trunk, Pyricularia urashimae on Urochloa brizantha , and Synnemellisia aurantia on Passiflora edulis . Chile : Tubulicrinis australis on Lophosoria quadripinnata. France : Cercophora squamulosa from submerged wood, and Scedosporium cereisporum from fluids of a wastewater treatment plant. Hawaii : Beltraniella acaciae , Dactylaria acaciae , Rhexodenticula acaciae , Rubikia evansii and Torula acaciae (all on Acacia koa ) . India : Lepidoderma echinosporum on dead semi-woody stems, and Rhodocybe rubrobrunnea from soil. Iran : Talaromyces kabodanensis from hypersaline soil. La Réunion : Neocordana musarum from leaves of Musa sp. Malaysia : Anungitea eucalyptigena on Eucalyptus grandis × pellita , Camptomeriphila leucaenae (incl. Camptomeriphila gen. nov.) on Leucaena leucocephala , Castanediella communis on Eucalyptus pellita , Eucalyptostroma eucalypti (incl. Eucalyptostroma gen. nov.) on Eucalyptus pellita , Melanconiella syzygii on Syzygium sp., Mycophilomyces periconiae (incl. Mycophilomyces gen. nov.) as hyperparasite on Periconia on leaves of Albizia falcataria , Synnemadiella eucalypti (incl. Synnemadiella gen. nov.) on Eucalyptus pellita , and Teichospora nephelii on Nephelium lappaceum. Mexico : Aspergillus bicephalus from soil. New Zealand : Aplosporella sophorae on Sophora microphylla , Libertasomyces platani on Platanus sp., Neothyronectria sophorae (incl. Neothyronectria gen. nov.) on Sophora microphylla , Parastagonospora phoenicicola on Phoenix canariensis , Phaeoacremonium pseudopanacis on Pseudopanax crassifolius , Phlyctema phoenicis on Phoenix canariensis , and Pseudoascochyta novae-zelandiae on Cordyline australis. Panama : Chalara panamensis from needle litter of Pinus cf. caribaea . South Africa : Exophiala eucalypti on leaves of Eucalyptus sp., Fantasmomyces hyalinus (incl. Fantasmomyces gen. nov.) on Acacia exuvialis , Paracladophialophora carceris (incl. Paracladophialophora gen. nov.) on Aloe sp., and Umthunziomyces hagahagensis (incl. Umthunziomyces gen. nov.) on Mimusops caffra. Spain : Clavaria griseobrunnea on bare ground in Pteridium aquilinum field, Cyathus ibericus on small fallen branches of Pinus halepensis , Gyroporus pseudolacteus in humus of Pinus pinaster , and Pseudoascochyta pratensis (incl. Pseudoascochyta gen. nov.) from soil. Thailand : Neoascochyta adenii on Adenium obesum , and Ochroconis capsici on Capsicum annuum . UK : Fusicolla melogrammae from dead stromata of Melogramma campylosporum on bark of Carpinus betulus . Uruguay : Myrmecridium pulvericola from house dust. USA : Neoscolecobasidium agapanthi (incl. Neoscolecobasidium gen. nov.) on Agapanthus sp . , Polyscytalum purgamentum on leaf litter, Pseudopithomyces diversisporus from human toenail, Saksenaea trapezispora from knee wound of a soldier, and Sirococcus quercus from Quercus sp . Morphological and culture characteristics along with DNA barcodes are provided.

Essential Oils of Myrtaceae Species Growing Wild in Tunisia: Chemical Variability and Antifungal Activity Against Biscogniauxia mediterranea, the Causative Agent of Charcoal Canker.

PubMed

Yangui, Islem; Zouaoui Boutiti, Meriem; Boussaid, Mohamed; Messaoud, Chokri

2017-07-01

The chemical composition of five Eucalyptus species and five Myrtus communis L. populations was investigated using GC/MS and GC-FID. For Eucalyptus essential oils, 32 compounds, representing 88.56 - 96.83% of the total oil according to species, were identified. The main compounds were 1,8-cineole, α-pinene, p-cymene, γ-gurjunene, α-aromadendrene, and β-phellandrene. For Myrtle essential oils, 26 compounds, representing 93.13 - 98.91% of the total oil were identified. α-Pinene, 1,8-cineole, linalool, and myrtenyl acetate were found to be the major compounds. Principal component analysis (PCA) showed chemical differentiation between Eucalyptus species and between Myrtle populations. Biscogniauxia mediterranea, the causative agent of charcoal canker, was identified according to its morphological and molecular characteristics. Essential oils of the investigated Eucalyptus species and Myrtle populations were tested for their antifungal capacity against this fungus. The antifungal activity varied according to the essential oil composition. Biscogniauxia mediterranea exhibited powerful resistance to some essential oils including them of Eucalyptus lehmannii and Eucalyptus sideroxylon but it was very sensitive to Eucalyptus camaldulensis oil (IC 50  = 3.83 mg/ml) and M. communis oil from Zaghouan (IC 50  = 1 mg/ml). This sensitivity was found to be correlated to some essential oil compounds such as p-cymene, carvacrol, cuminaldehyde, and linalool. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus.

PubMed

Qiao, Tian-Min; Zhang, Jing; Li, Shu-Jiang; Han, Shan; Zhu, Tian-Hui

2016-10-01

Eucalyptus dieback disease, caused by Cylindrocladium scoparium , has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium . The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

PubMed Central

Qiao, Tian-Min; Zhang, Jing; Li, Shu-Jiang; Han, Shan; Zhu, Tian-Hui

2016-01-01

Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products. PMID:27721691

The Eucalyptus terpene synthase gene family.

PubMed

Külheim, Carsten; Padovan, Amanda; Hefer, Charles; Krause, Sandra T; Köllner, Tobias G; Myburg, Alexander A; Degenhardt, Jörg; Foley, William J

2015-06-11

Terpenoids are abundant in the foliage of Eucalyptus, providing the characteristic smell as well as being valuable economically and influencing ecological interactions. Quantitative and qualitative inter- and intra- specific variation of terpenes is common in eucalypts. The genome sequences of Eucalyptus grandis and E. globulus were mined for terpene synthase genes (TPS) and compared to other plant species. We investigated the relative expression of TPS in seven plant tissues and functionally characterized five TPS genes from E. grandis. Compared to other sequenced plant genomes, Eucalyptus grandis has the largest number of putative functional TPS genes of any sequenced plant. We discovered 113 and 106 putative functional TPS genes in E. grandis and E. globulus, respectively. All but one TPS from E. grandis were expressed in at least one of seven plant tissues examined. Genomic clusters of up to 20 genes were identified. Many TPS are expressed in tissues other than leaves which invites a re-evaluation of the function of terpenes in Eucalyptus. Our data indicate that terpenes in Eucalyptus may play a wider role in biotic and abiotic interactions than previously thought. Tissue specific expression is common and the possibility of stress induction needs further investigation. Phylogenetic comparison of the two investigated Eucalyptus species gives insight about recent evolution of different clades within the TPS gene family. While the majority of TPS genes occur in orthologous pairs some clades show evidence of recent gene duplication, as well as loss of function.

Streptomyces rhizobacteria modulate the secondary metabolism of Eucalyptus plants.

PubMed

Salla, Tamiris Daros; da Silva, Ramos; Astarita, Leandro Vieira; Santarém, Eliane Romanato

2014-12-01

The genus Eucalyptus comprises economically important species, such as Eucalyptus grandis and Eucalyptus globulus, used especially as a raw material in many industrial sectors. Species of Eucalyptus are very susceptible to pathogens, mainly fungi, which leads to mortality of plant cuttings in rooting phase. One alternative to promote plant health and development is the potential use of microorganisms that act as agents for biological control, such as plant growth-promoting rhizobacteria (PGPR). Rhizobacteria Streptomyces spp have been considered as PGPR. This study aimed at selecting strains of Streptomyces with ability to promote plant growth and modulate secondary metabolism of E. grandis and E. globulus in vitro plants. The experiments assessed the development of plants (root number and length), changes in key enzymes in plant defense (polyphenol oxidase and peroxidase) and induction of secondary compounds(total phenolic and quercetinic flavonoid fraction). The isolate Streptomyces PM9 showed highest production of indol-3-acetic acid and the best potential for root induction. Treatment of Eucalyptus roots with Streptomyces PM9 caused alterations in enzymes activities during the period of co-cultivation (1-15 days), as well as in the levels of phenolic compounds and flavonoids. Shoots also showed alteration in the secondary metabolism, suggesting induced systemic response. The ability of Streptomyces sp. PM9 on promoting root growth, through production of IAA, and possible role on modulation of secondary metabolism of Eucalyptus plants characterizes this isolate as PGPR and indicates its potential use as a biological control in forestry.

Direct seeding of brushbox, lemon-gum eucalyptus, and cluster pine in Hawaii

Treesearch

Gerald A. Walters

1969-01-01

Seeds of brushbox, lemon-gum eucalyptus, and cluster pine were sown in separate seed spots on the Mokuleia Forest Reserve, Oahu. Half the seed spots were mulched. After 1 year, only two brushbox seed spots were stocked; lemon-gum eucalyptus had significantly (5 percent level) more seed spots stocked in the mulched plots; cluster pine had significantly less. These two...

Complex linkage between soil, soil water, atmosphere and Eucalyptus Plantations

NASA Astrophysics Data System (ADS)

Shukla, C.; Tiwari, K. N.

2017-12-01

Eucalyptus is most widely planted genus grown in waste land of eastern region of India to meet the pulp industry requirements. Sustainability of these plantations is of concern because in spite of higher demand water and nutrients of plantations, they are mostly planted on low-fertility soils. This study has been conducted to quantify effect of 25 years old, a fully established eucalyptus plantations on i.) Alteration in physico-chemical and hydrological properties of soil of eucalyptus plantation in comparison to soil of natural grassland and ii.) Spatio-temporal variation in soil moisture under eucalyptus plantations. Soil physico-chemical properties of two adjacent plots covered with eucatuptus and natural grasses were analyzed for three consecutive depths (i.e. 0-30 cm, 30-60 cm and 60-90 cm) with five replications in each plot. Soil infiltration rate and saturated hydraulic conductivity (Ks) were measured in-situ to incorporate the influence of macro porosity caused due to roots of plantations. Daily soil moisture at an interval of 10 cm upto 160 cm depth with 3 replications and Leaf Area Index (LAI) at an interval of 15 days with 5 replications were recorded over the year. Significant variations found at level of 0.05 between soil properties of eucalyptus and natural grass land confirm the effect of plantations on soil properties. Comparative results of soil properties show significant alteration in soil texture such as percent of sand, organic matter and Ks found more by 20%, 9% and 22% respectively in eucalyptus plot as compare to natural grass land. Available soil moisture (ASM) was found constantly minimum in top soil excluding rainy season indicate upward movement of water and nutrients during dry season. Seasonal variation in temperature (T), relative humidity (RH) and leaf area index (LAI) influenced the soil moisture extraction phenomenon. This study clearly stated the impact of long term establishment of eucalyptus plantations make considerable alteration on soil texture and Ks. Also, relationship between T, RH, LAI and ASM developed can be used for soil moisture modelling for watersheds with eucalyptus plantations.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

Billerud signed a contract recently with the Societe d'Etudes de la Celulose du Congo (SECC) to conduct a feasibility study for a projected eucalyptus-based pulp plant for the production of bleached marketing pulp in The People's Republic of The Congo. SECC is a consortium of financial institutions, the Congolese government and Billerud. The Congolese government has been planning for the construction of a eucalyptus-based pulp plant for a long time, and in 1978, full-scale eucalyptus plantations were started. These plantations are based on fast growing hybrid species of eucalyptus developed by the Centre Technique Forestier Tropical in the Congo andmore » are ready for harvesting after only about seven years growing time.« less

Maternal inheritance of the chloroplast genome in Eucalyptus globulus and interspecific hybrids.

PubMed

Mckinnon, A E; Vaillancourt, R E; Tilyard, P A; Potts, B M

2001-10-01

The utility of chloroplast DNA (cpDNA) in Eucalyptus, either as a molecular marker for genetic studies or as a potential vehicle for genetic manipulation, is based on knowledge of its mode of inheritance. Chloroplast inheritance in angiosperms can vary among and within species, and anomalous inheritance has been reported in some interspecific-hybrid combinations. In Eucalyptus, abnormalities of pollen-tube growth occur in a number of interspecific-hybrid combinations, and this might increase the likelihood of anomalous chloroplast transmission. We used a rapid PCR technique to determine chloroplast heritability in 425 progeny of Eucalyptus, comprising 194 progeny of the premier pulpwood species E. globulus and 231 interspecific hybrids between E. globulus and E. nitens (F1, F2, and backcrosses). At this sampling intensity, no pollen-mediated transmission of cpDNA was found in any of the 40 families tested. The results are discussed with reference to chloroplast engineering and the use of cpDNA as a seed-specific marker in phylogeographic studies of Eucalyptus.

Fungal Planet description sheets: 69-91.

PubMed

Crous, P W; Groenewald, J Z; Shivas, R G; Edwards, J; Seifert, K A; Alfenas, A C; Alfenas, R F; Burgess, T I; Carnegie, A J; Hardy, G E St J; Hiscock, N; Hüberli, D; Jung, T; Louis-Seize, G; Okada, G; Pereira, O L; Stukely, M J C; Wang, W; White, G P; Young, A J; McTaggart, A R; Pascoe, I G; Porter, I J; Quaedvlieg, W

2011-06-01

Novel species of microfungi described in the present study include the following from Australia: Bagadiella victoriae and Bagadiella koalae on Eucalyptus spp., Catenulostroma eucalyptorum on Eucalyptus laevopinea, Cercospora eremochloae on Eremochloa bimaculata, Devriesia queenslandica on Scaevola taccada, Diaporthe musigena on Musa sp., Diaporthe acaciigena on Acacia retinodes, Leptoxyphium kurandae on Eucalyptus sp., Neofusicoccum grevilleae on Grevillea aurea, Phytophthora fluvialis from water in native bushland, Pseudocercospora cyathicola on Cyathea australis, and Teratosphaeria mareebensis on Eucalyptus sp. Other species include Passalora leptophlebiae on Eucalyptus leptophlebia (Brazil), Exophiala tremulae on Populus tremuloides and Dictyosporium stellatum from submerged wood (Canada), Mycosphaerella valgourgensis on Yucca sp. (France), Sclerostagonospora cycadis on Cycas revoluta (Japan), Rachicladosporium pini on Pinus monophylla (Netherlands), Mycosphaerella wachendorfiae on Wachendorfia thyrsifolia and Diaporthe rhusicola on Rhus pendulina (South Africa). Novel genera of hyphomycetes include Noosia banksiae on Banksia aemula (Australia), Utrechtiana cibiessia on Phragmites australis (Netherlands), and Funbolia dimorpha on blackened stem bark of an unidentified tree (USA). Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa.

Variation in chemical composition and acaricidal activity against Dermanyssus gallinae of four eucalyptus essential oils.

PubMed

George, David R; Masic, Dino; Sparagano, Olivier A E; Guy, Jonathan H

2009-06-01

The results of this study suggest that certain eucalyptus essential oils may be of use as an alternative to synthetic acaricides in the management of the poultry red mite, Dermanyssus gallinae. At a level of 0.21 mg/cm(2), the essential oil from Eucalyptus citriodora achieved 85% mortality in D. gallinae over a 24 h exposure period in contact toxicity tests. A further two essential oils from different eucalyptus species, namely E. globulus and E. radiata, provided significantly (P < 0.05) lower mite mortality (11 and 19%, respectively). Notable differences were found between the eucalyptus essential oils regarding their chemical compositions. There appeared to be a trend whereby the essential oils that were composed of the fewer chemical components were the least lethal to D. gallinae. It may therefore be the case that the complexity of an essential oil's chemical make up plays an important role in dictating the toxicity of that oil to pests such as D. gallinae.

Three new species of Fergusonina Malloch fly (Diptera: Fergusoninidae) from bud galls on Eucalyptus L'Her. (E. baxteri (Benth.) Maiden & Blakely complex, E. dalrympleana Maiden and E. pauciflora Sieb. ex Spreng.)

USDA-ARS?s Scientific Manuscript database

Three Fergusonina (Diptera: Fergusoninidae) flies are described from terminal leaf bud galls on Eucalyptus L'Hér. from south eastern Australia. Fergusonina omlandi Nelson and Yeates sp. nov. is the third fly from the genus Fergusonina to be described from the Eucalyptus pauciflora Sieb. ex Spreng. (...

Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus.

PubMed

Mizrachi, Eshchar; Maloney, Victoria J; Silberbauer, Janine; Hefer, Charles A; Berger, Dave K; Mansfield, Shawn D; Myburg, Alexander A

2015-06-01

Tension wood has distinct physical and chemical properties, including altered fibre properties, cell wall composition and ultrastructure. It serves as a good system for investigating the genetic regulation of secondary cell wall biosynthesis and wood formation. The reference genome sequence for Eucalyptus grandis allows investigation of the global transcriptional reprogramming that accompanies tension wood formation in this global wood fibre crop. We report the first comprehensive analysis of physicochemical wood property changes in tension wood of Eucalyptus measured in a hybrid (E. grandis × Eucalyptus urophylla) clone, as well as genome-wide gene expression changes in xylem tissues 3 wk post-induction using RNA sequencing. We found that Eucalyptus tension wood in field-grown trees is characterized by an increase in cellulose, a reduction in lignin, xylose and mannose, and a marked increase in galactose. Gene expression profiling in tension wood-forming tissue showed corresponding down-regulation of monolignol biosynthetic genes, and differential expression of several carbohydrate active enzymes. We conclude that alterations of cell wall traits induced by tension wood formation in Eucalyptus are a consequence of a combination of down-regulation of lignin biosynthesis and hemicellulose remodelling, rather than the often proposed up-regulation of the cellulose biosynthetic pathway. © 2014 University of Pretoria New Phytologist © 2014 New Phytologist Trust.

Efficacy of spray formulations containing binary mixtures of clove and eucalyptus oils against susceptible and pyrethroid/ malathion-resistant head lice (Anoplura: Pediculidae).

PubMed

Choi, Han-Young; Yang, Young-Cheol; Lee, Si Hyeock; Clark, J Marshall; Ahn, Young-Joon

2010-05-01

The control efficacy of clove, Eugenia caryophyllata, and eucalyptus, Eucalyptus globulus, essential oils and 15 formulations containing these essential oils alone (8, 12, and 15% sprays) and their binary mixtures (7:3, 5:5, and 3:7 by weight) against adult females of insecticide-susceptible KR-HL and dual malathion- and permethrin-resistant BR-HL strains of head louse, Pediculus humanus capitis (De Geer), was examined by using contact plus fumigant and human hair wig (placed over the head of mannequin) mortality bioassays. In contact plus fumigant mortality bioassay, essential oils from eucalyptus (0.225 mg/cm2) and clove (1.149 mg/cm2) were less effective than either d-phenothrin (0.0029 mg/cm2) or pyrethrum (0.0025 mg/cm2) based on 6-h median lethal concentration values. However, the efficacies of eucalyptus and clove oils were almost identical against females fromn both strains, despite high levels of resistance of the BR-HL females to d-phenothrin (resistance ratio, 667) and pyrethrum (resistance ratio, 754). In human hair wig mortality bioassay, eucalyptus oil spray treatment gave better control efficacy than either spray treatment with clove oil alone or their binary mixtures. Thus, eucalyptus applied as 8% sprays (15 or 20 ml) appears to provide effective protection against pediculosis even to insecticide-resistant head louse populations. Once the safety issues resolved, covering the treated hair and scalp with bath shower cap or hat would ensure the fumigant action of the essential oil.

Transcriptome and proteome analysis of Eucalyptus infected with Calonectria pseudoreteaudii.

PubMed

Chen, Quanzhu; Guo, Wenshuo; Feng, Lizhen; Ye, Xiaozhen; Xie, Wanfeng; Huang, Xiuping; Liu, Jinyan

2015-02-06

Cylindrocladium leaf blight is one of the most severe diseases in Eucalyptus plantations and nurseries. There are Eucalyptus cultivars with resistance to the disease. However, little is known about the defense mechanism of resistant cultivars. Here, we investigated the transcriptome and proteome of Eucalyptus leaves (E. urophylla×E. tereticornis M1), infected or not with Calonectria pseudoreteaudii. A total of 8585 differentially expressed genes (|log2 ratio| ≥1, FDR ≤0.001) at 12 and 24hours post-inoculation were detected using RNA-seq. Transcriptional changes for five genes were further confirmed by qRT-PCR. A total of 3680 proteins at the two time points were identified using iTRAQ technique.The combined transcriptome and proteome analysis revealed that the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway (jasmonic acid and sugar) were activated. The data also showed that some proteins (WRKY33 and PR proteins) which have been reported to involve in plant defense response were up-regulated. However, photosynthesis, nucleic acid metabolism and protein metabolism were impaired by the infection of C. pseudoreteaudii. This work will facilitate the identification of defense related genes and provide insights into Eucalyptus defense responses to Cylindrocladium leaf blight. In this study, a total of 130 proteins and genes involved in the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway, cell transport, carbohydrate and energy metabolism, nucleic acid metabolism and protein metabolism in Eucalyptus leaves after infected with C. pseudoreteaudii were identified. This is the first report of a comprehensive transcriptomic and proteomic analysis of Eucalyptus in response to Calonectria sp. Copyright © 2014 Elsevier B.V. All rights reserved.

Effects of the interception of litterfall by the understory on carbon cycling in eucalyptus plantations of South China.

PubMed

Yang, Long; Wang, Jun; Huang, Yuhui; Hui, Dafeng; Wen, Meili

2014-01-01

For the purposes of forest restoration, carbon (C) fixation, and economic improvement, eucalyptus (Eucalyptus urophylla) has been widely planted in South China. The understory of eucalyptus plantations is often occupied by a dense community of the fern Dicranopteris dichotoma, which intercepts tree canopy leaf litter before it reaches the ground. To understand the effects of this interception of litterfall on C cycling in eucalyptus plantations, we quantified the mass of intercepted litter and the influences of litterfall interception on litter decomposition and soil respiration. The total mass of E. urophylla litterfall collected on the understory was similar to that collected by the traditional litter trap method. All of the eucalyptus litterfall is intercepted by the D. dichotoma canopy. Of the litterfall that was intercepted by D. dichotoma, 20-40% and 60-80% was intercepted by the top (50-100 cm) and bottom (0-50 cm) of the understory canopy, respectively. Intercepted litterfall decomposed faster at the bottom of understory canopy (at the base of the plants) than at the top, and decomposition was slower on the soil surface in the absence of understory than on any location in the understory canopy. Soil respiration was highest when both the understory and litter were present and was lowest when both the understory and litter were absent. These results indicate that litterfall interception changed carbon flow between aboveground and belowground through litter decomposition and soil respiration, which changed carbon cycling in eucalyptus plantations. The effects of the understory on litter decomposition and soil respiration should be considered in ecosystem carbon models.

Fungal Planet description sheets: 320-370.

PubMed

Crous, P W; Wingfield, M J; Guarro, J; Hernández-Restrepo, M; Sutton, D A; Acharya, K; Barber, P A; Boekhout, T; Dimitrov, R A; Dueñas, M; Dutta, A K; Gené, J; Gouliamova, D E; Groenewald, M; Lombard, L; Morozova, O V; Sarkar, J; Smith, M Th; Stchigel, A M; Wiederhold, N P; Alexandrova, A V; Antelmi, I; Armengol, J; Barnes, I; Cano-Lira, J F; Castañeda Ruiz, R F; Contu, M; Courtecuisse, Pr R; da Silveira, A L; Decock, C A; de Goes, A; Edathodu, J; Ercole, E; Firmino, A C; Fourie, A; Fournier, J; Furtado, E L; Geering, A D W; Gershenzon, J; Giraldo, A; Gramaje, D; Hammerbacher, A; He, X-L; Haryadi, D; Khemmuk, W; Kovalenko, A E; Krawczynski, R; Laich, F; Lechat, C; Lopes, U P; Madrid, H; Malysheva, E F; Marín-Felix, Y; Martín, M P; Mostert, L; Nigro, F; Pereira, O L; Picillo, B; Pinho, D B; Popov, E S; Rodas Peláez, C A; Rooney-Latham, S; Sandoval-Denis, M; Shivas, R G; Silva, V; Stoilova-Disheva, M M; Telleria, M T; Ullah, C; Unsicker, S B; van der Merwe, N A; Vizzini, A; Wagner, H-G; Wong, P T W; Wood, A R; Groenewald, J Z

2015-06-01

Novel species of fungi described in the present study include the following from Malaysia: Castanediella eucalypti from Eucalyptus pellita, Codinaea acacia from Acacia mangium, Emarcea eucalyptigena from Eucalyptus brassiana, Myrtapenidiella eucalyptorum from Eucalyptus pellita, Pilidiella eucalyptigena from Eucalyptus brassiana and Strelitziana malaysiana from Acacia mangium. Furthermore, Stachybotrys sansevieriicola is described from Sansevieria ehrenbergii (Tanzania), Phacidium grevilleae from Grevillea robusta (Uganda), Graphium jumulu from Adansonia gregorii and Ophiostoma eucalyptigena from Eucalyptus marginata (Australia), Pleurophoma ossicola from bone and Plectosphaerella populi from Populus nigra (Germany), Colletotrichum neosansevieriae from Sansevieria trifasciata, Elsinoë othonnae from Othonna quinquedentata and Zeloasperisporium cliviae (Zeloasperisporiaceae fam. nov.) from Clivia sp. (South Africa), Neodevriesia pakbiae, Phaeophleospora hymenocallidis and Phaeophleospora hymenocallidicola on leaves of a fern (Thailand), Melanconium elaeidicola from Elaeis guineensis (Indonesia), Hormonema viticola from Vitis vinifera (Canary Islands), Chlorophyllum pseudoglobossum from a grassland (India), Triadelphia disseminata from an immunocompromised patient (Saudi Arabia), Colletotrichum abscissum from Citrus (Brazil), Polyschema sclerotigenum and Phialemonium limoniforme from human patients (USA), Cadophora vitícola from Vitis vinifera (Spain), Entoloma flavovelutinum and Bolbitius aurantiorugosus from soil (Vietnam), Rhizopogon granuloflavus from soil (Cape Verde Islands), Tulasnella eremophila from Euphorbia officinarum subsp. echinus (Morocco), Verrucostoma martinicensis from Danaea elliptica (French West Indies), Metschnikowia colchici from Colchicum autumnale (Bulgaria), Thelebolus microcarpus from soil (Argentina) and Ceratocystis adelpha from Theobroma cacao (Ecuador). Myrmecridium iridis (Myrmecridiales ord. nov., Myrmecridiaceae fam. nov.) is also described from Iris sp. (The Netherlands). Novel genera include (Ascomycetes): Budhanggurabania from Cynodon dactylon (Australia), Soloacrosporiella, Xenocamarosporium, Neostrelitziana and Castanediella from Acacia mangium and Sabahriopsis from Eucalyptus brassiana (Malaysia), Readerielliopsis from basidiomata of Fuscoporia wahlbergii (French Guyana), Neoplatysporoides from Aloe ferox (Tanzania), Wojnowiciella, Chrysofolia and Neoeriomycopsis from Eucalyptus (Colombia), Neophaeomoniella from Eucalyptus globulus (USA), Pseudophaeomoniella from Olea europaea (Italy), Paraphaeomoniella from Encephalartos altensteinii, Aequabiliella, Celerioriella and Minutiella from Prunus (South Africa). Tephrocybella (Basidiomycetes) represents a novel genus from wood (Italy). Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa.

Fungal Planet description sheets: 625-715.

PubMed

Crous, P W; Wingfield, M J; Burgess, T I; Carnegie, A J; Hardy, G E St J; Smith, D; Summerell, B A; Cano-Lira, J F; Guarro, J; Houbraken, J; Lombard, L; Martín, M P; Sandoval-Denis, M; Alexandrova, A V; Barnes, C W; Baseia, I G; Bezerra, J D P; Guarnaccia, V; May, T W; Hernández-Restrepo, M; Stchigel, A M; Miller, A N; Ordoñez, M E; Abreu, V P; Accioly, T; Agnello, C; Agustin Colmán, A; Albuquerque, C C; Alfredo, D S; Alvarado, P; Araújo-Magalhães, G R; Arauzo, S; Atkinson, T; Barili, A; Barreto, R W; Bezerra, J L; Cabral, T S; Camello Rodríguez, F; Cruz, R H S F; Daniëls, P P; da Silva, B D B; de Almeida, D A C; de Carvalho Júnior, A A; Decock, C A; Delgat, L; Denman, S; Dimitrov, R A; Edwards, J; Fedosova, A G; Ferreira, R J; Firmino, A L; Flores, J A; García, D; Gené, J; Giraldo, A; Góis, J S; Gomes, A A M; Gonçalves, C M; Gouliamova, D E; Groenewald, M; Guéorguiev, B V; Guevara-Suarez, M; Gusmão, L F P; Hosaka, K; Hubka, V; Huhndorf, S M; Jadan, M; Jurjević, Ž; Kraak, B; Kučera, V; Kumar, T K A; Kušan, I; Lacerda, S R; Lamlertthon, S; Lisboa, W S; Loizides, M; Luangsa-Ard, J J; Lysková, P; Mac Cormack, W P; Macedo, D M; Machado, A R; Malysheva, E F; Marinho, P; Matočec, N; Meijer, M; Mešić, A; Mongkolsamrit, S; Moreira, K A; Morozova, O V; Nair, K U; Nakamura, N; Noisripoom, W; Olariaga, I; Oliveira, R J V; Paiva, L M; Pawar, P; Pereira, O L; Peterson, S W; Prieto, M; Rodríguez-Andrade, E; Rojo De Blas, C; Roy, M; Santos, E S; Sharma, R; Silva, G A; Souza-Motta, C M; Takeuchi-Kaneko, Y; Tanaka, C; Thakur, A; Smith, M Th; Tkalčec, Z; Valenzuela-Lopez, N; van der Kleij, P; Verbeken, A; Viana, M G; Wang, X W; Groenewald, J Z

2017-12-01

Novel species of fungi described in this study include those from various countries as follows: Antarctica : Cadophora antarctica from soil. Australia : Alfaria dandenongensis on Cyperaceae , Amphosoma persooniae on Persoonia sp., Anungitea nullicana on Eucalyptus sp . , Bagadiella eucalypti on Eucalyptus globulus , Castanediella eucalyptigena on Eucalyptus sp., Cercospora dianellicola on Dianella sp., Cladoriella kinglakensis on Eucalyptus regnans , Cladoriella xanthorrhoeae (incl. Cladoriellaceae fam. nov. and Cladoriellales ord. nov.) on Xanthorrhoea sp., Cochlearomyces eucalypti (incl. Cochlearomyces gen. nov. and Cochlearomycetaceae fam. nov.) on Eucalyptus obliqua , Codinaea lambertiae on Lambertia formosa , Diaporthe obtusifoliae on Acacia obtusifolia , Didymella acaciae on Acacia melanoxylon , Dothidea eucalypti on Eucalyptus dalrympleana , Fitzroyomyces cyperi (incl. Fitzroyomyces gen. nov.) on Cyperaceae , Murramarangomyces corymbiae (incl. Murramarangomyces gen. nov., Murramarangomycetaceae fam. nov. and Murramarangomycetales ord. nov.) on Corymbia maculata , Neoanungitea eucalypti (incl. Neoanungitea gen. nov.) on Eucalyptus obliqua , Neoconiothyrium persooniae (incl. Neoconiothyrium gen. nov.) on Persoonia laurina subsp. laurina , Neocrinula lambertiae (incl. Neocrinulaceae fam. nov.) on Lambertia sp., Ochroconis podocarpi on Podocarpus grayae , Paraphysalospora eucalypti (incl. Paraphysalospora gen. nov.) on Eucalyptus sieberi , Pararamichloridium livistonae (incl. Pararamichloridium gen. nov., Pararamichloridiaceae fam. nov. and Pararamichloridiales ord. nov.) on Livistona sp., Pestalotiopsis dianellae on Dianella sp., Phaeosphaeria gahniae on Gahnia aspera , Phlogicylindrium tereticornis on Eucalyptus tereticornis , Pleopassalora acaciae on Acacia obliquinervia , Pseudodactylaria xanthorrhoeae (incl. Pseudodactylaria gen. nov., Pseudodactylariaceae fam. nov. and Pseudodactylariales ord. nov.) on Xanthorrhoea sp., Pseudosporidesmium lambertiae (incl. Pseudosporidesmiaceae fam. nov.) on Lambertia formosa , Saccharata acaciae on Acacia sp., Saccharata epacridis on Epacris sp., Saccharata hakeigena on Hakea sericea , Seiridium persooniae on Persoonia sp., Semifissispora tooloomensis on Eucalyptus dunnii , Stagonospora lomandrae on Lomandra longifolia , Stagonospora victoriana on Poaceae , Subramaniomyces podocarpi on Podocarpus elatus , Sympoventuria melaleucae on Melaleuca sp . , Sympoventuria regnans on Eucalyptus regnans , Trichomerium eucalypti on Eucalyptus tereticornis , Vermiculariopsiella eucalypticola on Eucalyptus dalrympleana , Verrucoconiothyrium acaciae on Acacia falciformis , Xenopassalora petrophiles (incl. Xenopassalora gen. nov.) on Petrophile sp . , Zasmidium dasypogonis on Dasypogon sp., Zasmidium gahniicola on Gahnia sieberiana. Brazil : Achaetomium lippiae on Lippia gracilis , Cyathus isometricus on decaying wood, Geastrum caririense on soil, Lycoperdon demoulinii (incl. Lycoperdon subg. Arenicola ) on soil, Megatomentella cristata (incl. Megatomentella gen. nov.) on unidentified plant, Mutinus verrucosus on soil, Paraopeba schefflerae (incl. Paraopeba gen. nov.) on Schefflera morototoni , Phyllosticta catimbauensis on Mandevilla catimbauensis , Pseudocercospora angularis on Prunus persica , Pseudophialophora sorghi on Sorghum bicolor , Spumula piptadeniae on Piptadenia paniculata. Bulgaria : Yarrowia parophonii from gut of Parophonus hirsutulus . Croatia : Pyrenopeziza velebitica on Lonicera borbasiana. Cyprus : Peziza halophila on coastal dunes. Czech Republic : Aspergillus contaminans from human fingernail. Ecuador : Cuphophyllus yacurensis on forest soil, Ganoderma podocarpense on fallen tree trunk. England : Pilidium anglicum (incl. Chaetomellales ord. nov.) on Eucalyptus sp. France : Planamyces parisiensis (incl. Planamyces gen. nov.) on wood inside a house. French Guiana : Lactifluus ceraceus on soil. Germany : Talaromyces musae on Musa sp. India : Hyalocladosporiella cannae on Canna indica , Nothophoma raii from soil. Italy : Setophaeosphaeria citri on Citrus reticulata , Yuccamyces citri on Citrus limon. Japan : Glutinomyces brunneus (incl. Glutinomyces gen. nov.) from roots of Quercus sp. Netherlands (all from soil): Collariella hilkhuijsenii , Fusarium petersiae , Gamsia kooimaniorum , Paracremonium binnewijzendii , Phaeoisaria annesophieae , Plectosphaerella niemeijerarum , Striaticonidium deklijnearum , Talaromyces annesophieae , Umbelopsis wiegerinckiae , Vandijckella johannae (incl. Vandijckella gen. nov. and Vandijckellaceae fam. nov.), Verhulstia trisororum (incl. Verhulstia gen. nov.). New Zealand : Lasiosphaeria similisorbina on decorticated wood. Papua New Guinea : Pseudosubramaniomyces gen. nov. (based on Pseudosubramaniomyces fusisaprophyticus comb. nov.). Slovakia : Hemileucoglossum pusillum on soil. South Africa : Tygervalleyomyces podocarpi (incl. Tygervalleyomyces gen. nov.) on Podocarpus falcatus. Spain : Coniella heterospora from herbivorous dung, Hymenochaete macrochloae on Macrochloa tenacissima , Ramaria cistophila on shrubland of Cistus ladanifer. Thailand : Polycephalomyces phaothaiensis on Coleoptera larvae, buried in soil. Uruguay : Penicillium uruguayense from soil. Vietnam : Entoloma nigrovelutinum on forest soil, Volvariella morozovae on wood of unknown tree. Morphological and culture characteristics along with DNA barcodes are provided.

Study on the Modification of Bleached Eucalyptus Kraft Pulp Using Birch Xylan

Treesearch

Wenjia Han; Chuanshan Zhao; Thomas Elder; Rendang Yang; Dongho Kim; Yunqiao Pu; Jeffery Hsieh; Arthur J. Ragauskas

2012-01-01

In this study, birch xylan was deposited onto elementally chlorine free (ECF) bleached eucalyptus kraft pulp, and the corresponding changes in physical properties were determined. An aqueous 5% birch xylan solution at pH 9 was added to 5 wt% slurry of bleached kraft eucalyptus fibers, with stirring at 70 C for 15 min after which the pH was adjusted to 5–6. The xylan...

Methanol production from Eucalyptus wood chips. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fishkind, H.H.

This feasibility study includes all phases of methanol production from seedling to delivery of finished methanol. The study examines: production of 55 million, high quality, Eucalyptus seedlings through tissue culture; establishment of a Eucalyptus energy plantation on approximately 70,000 acres; engineering for a 100 million gallon-per-day methanol production facility; potential environmental impacts of the whole project; safety and health aspects of producing and using methanol; and development of site specific cost estimates.

Effects of the Interception of Litterfall by the Understory on Carbon Cycling in Eucalyptus Plantations of South China

PubMed Central

Huang, Yuhui; Hui, Dafeng; Wen, Meili

2014-01-01

For the purposes of forest restoration, carbon (C) fixation, and economic improvement, eucalyptus (Eucalyptus urophylla) has been widely planted in South China. The understory of eucalyptus plantations is often occupied by a dense community of the fern Dicranopteris dichotoma, which intercepts tree canopy leaf litter before it reaches the ground. To understand the effects of this interception of litterfall on C cycling in eucalyptus plantations, we quantified the mass of intercepted litter and the influences of litterfall interception on litter decomposition and soil respiration. The total mass of E. urophylla litterfall collected on the understory was similar to that collected by the traditional litter trap method. All of the eucalyptus litterfall is intercepted by the D. dichotoma canopy. Of the litterfall that was intercepted by D. dichotoma, 20–40% and 60–80% was intercepted by the top (50–100 cm) and bottom (0–50 cm) of the understory canopy, respectively. Intercepted litterfall decomposed faster at the bottom of understory canopy (at the base of the plants) than at the top, and decomposition was slower on the soil surface in the absence of understory than on any location in the understory canopy. Soil respiration was highest when both the understory and litter were present and was lowest when both the understory and litter were absent. These results indicate that litterfall interception changed carbon flow between aboveground and belowground through litter decomposition and soil respiration, which changed carbon cycling in eucalyptus plantations. The effects of the understory on litter decomposition and soil respiration should be considered in ecosystem carbon models. PMID:24959853

BVOC emissions from 2 Asian Eucalyptus species,E.camadulensis and E.robusta

NASA Astrophysics Data System (ADS)

Tsui, J.; Guenther, A. B.; Chan, C. K.; Lau, A. P.

2009-12-01

Eucalyptus species dominate native forests in Australia and are planted over vast regions in Asia and other continents for afforestation and for pulp due to their fast growth rates. However, they have also been identified as high emitters of biogenic volatile organic compounds (BVOCs). BVOCs, when emitted to the atmosphere, react to form air pollutants such as ozone (O3) and secondary organic aerosols (SOA). The large areas of Eucalyptus forests in Australia and Asia, and high BVOC emission rates of Eucalyptus species, imply a potential significant effect of BVOC emissions from Eucalyptus on the air quality of these regions. A better understanding of BVOC emissions from this genus is thus needed. Here we present data of BVOC measurements from E.camadulensis and E.robusta. BVOC emissions of the 2 Eucalyptus species were measured by a branch enclosure approach in an environmental chamber, in which light and temperature were carefully controlled to mimic their changes throughout the day under natural conditions. E. camadulensis was found to emit isoprene, α-pinene, camphene and limonene, while E. robusta was found to emit isoprene, α-pinene, β-pinene, α-phellandrene, 3-carene and ocimene. Diurnal variations in BVOC emissions from the 2 species were observed. The 2 Eucalyptus species were also treated with methyl jasmonate (MeJA), a plant hormone which has found to induce elevated BVOC emissions similar to response to insect attacks in other plant species. The emission profiles of the 2 species before and after MeJA treatment were contrasted to examine the effects of the MeJA on their BVOC emissions. Acknowledgment: This work was supported by the General Research Fund of the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. 610909).

Chemical composition and antibacterial activities of seven Eucalyptus species essential oils leaves.

PubMed

Sebei, Khaled; Sakouhi, Fawzi; Herchi, Wahid; Khouja, Mohamed Larbi; Boukhchina, Sadok

2015-01-19

In this paper, we have studied the essential oils chemical composition of the leaves of seven Eucalyptus species developed in Tunisia. Eucalyptus leaves were picked from trees growing in different arboretums in Tunisia. Choucha and Mrifeg arboretums located in Sedjnene, region of Bizerte (Choucha: E. maideni, E. astrengens et E. cinerea; Mrifeg : E. leucoxylon), Korbous arboretums located in the region of Nabeul, North East Tunisia with sub-humid bioclimate, (E. lehmani), Souiniet-Ain Drahem arboretum located in region of Jendouba (E. sideroxylon, E. bicostata). Essential oils were individually tested against a large panel of microorganisms including Staphylococcus aureus (ATCC 6539), Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC29212), Listeria ivanovii (RBL 30), Bacillus cereus (ATCC11778). The yield of essential oils ranged from 1.2% to 3% (w/w) for the different Eucalyptus species. All essential oils contain α-pinene, 1,8-cineol and pinocarveol-trans for all Eucalyptus species studied. The 1,8-cineol was the major compound in all species (49.07 to 83.59%). Diameter of inhibition zone of essential oils of Eucalyptus species varied from 10 to 29 mm. The largest zone of inhibition was obtained for Bacillus cereus (E. astrengens) and the lowest for Staphylococcus aureus (E. cinerea). The essential oils from E. maideni, E. astrengens, E. cinerea (arboretum of Bizerte), E. bicostata (arboretum of Aindraham) showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus. The major constituents of Eucalyptus leaves essential oils are 1,8-cineol (49.07 to 83.59%) and α-pinene (1.27 to 26.35%). The essential oils from E. maideni, E. astrengens, E. cinerea, E. bicostata showed the highest antibacterial activity against Listeria ivanovii and Bacillus cereus, they may have potential applications in food and pharmaceutical products.

Environmental effects on growth phenology of co-occurring Eucalyptus species.

PubMed

Rawal, Deepa S; Kasel, Sabine; Keatley, Marie R; Aponte, Cristina; Nitschke, Craig R

2014-05-01

Growth is one of the most important phenological cycles in a plant's life. Higher growth rates increase the competitive ability, survival and recruitment and can provide a measure of a plant's adaptive capacity to climate variability and change. This study identified the growth relationship of six Eucalyptus species to variations in temperature, soil moisture availability, photoperiod length and air humidity over 12 months. The six species represent two naturally co-occurring groups of three species each representing warm-dry and the cool-moist sclerophyll forests, respectively. Warm-dry eucalypts were found to be more tolerant of higher temperatures and lower air humidity than the cool-moist eucalypts. Within groups, species-specific responses were detected with Eucalyptus microcarpa having the widest phenological niche of the warm-dry species, exhibiting greater resistance to high temperature and lower air humidity. Temperature dependent photoperiodic responses were exhibited by all the species except Eucalyptus tricarpa and Eucalyptus sieberi, which were able to maintain growth as photoperiod shortened but temperature requirements were fulfilled. Eucalyptus obliqua exhibited a flexible growth rate and tolerance to moisture limitation which enables it to maintain its growth rate as water availability changes. The wider temperature niche exhibited by E. sieberi compared with E. obliqua and Eucalyptus radiata may improve its competitive ability over these species where winters are warm and moisture does not limit growth. With climate change expected to result in warmer and drier conditions in south-east Australia, the findings of this study suggest all cool-moist species will likely suffer negative effects on growth while the warm-dry species may still maintain current growth rates. Our findings highlight that climate driven shifts in growth phenology will likely occur as climate changes and this may facilitate changes in tree communities by altering inter-specific competition.

The gall wasp Leptocybe invasa (Hymenoptera: Eulophidae) stimulates different chemical and phytohormone responses in two Eucalyptus varieties that vary in susceptibility to galling.

PubMed

Li, X Q; Liu, Y Z; Guo, W F; Solanki, M K; Yang, Z D; Xiang, Y; Ma, Z C; Wen, Y G

2017-09-01

Gall-inducing insects produce various types of galls on plants, but little is known about the gall-induction mechanism of these galling insects. The gall wasp Leptocybe invasa Fisher & LaSalle (Hymenoptera: Eulophidae) forms galls of different sizes on several Eucalyptus species. To clarify the physiological responses of Eucalyptus to L. invasa infestation, we measured the dynamics of nitrogen (N), carbon (C), total phenolics, total tannins and four types of phytohormones (zeatin [Z] + zeatin riboside [ZR], gibberellins [GA], indole-3-acetic acid [IAA] and abscisic acid [ABA]) in galled and ungalled leaf tissues of two Eucalyptus horticultural varieties (DH201-2 [Eucalyptus grandis × Eucalyptus camaldulensis] and EA [Eucalyptus exserta]) with different susceptibility to galling throughout the larval developmental stages. Nitrogen, total phenolics, tannins and four kinds of phytohormones strongly accumulated in tissues galled by L. invasa (especially during early larval feeding stages). While N, Z + ZR and GA levels were higher, tannins and ABA levels were lower in the galled tissues on the highly susceptible variety. Nitrogen, total phenolics, GA, Z + ZR and IAA levels in the galled tissues gradually decreased during gall development, but ABA and tannins conversely increased in the galled tissues of the less susceptible variety. Our results suggest that the effects of gall-inducing insects on plants depend not only on the susceptibility of the plant infested but also on the developmental stage of galled tissues. Gall formation process is thus synergistically influenced by both gall-inducing insect and plant genotypes. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Saligna eucalyptus growth in a 15-year old spacing study in Hawaii

Treesearch

Gerald A. Walters

1980-01-01

A spacing study was started in 1961 to test the effects of four different spacings on the growth and development of saligna eucalyptus (Eucalyptus saligna Smith) trees in Hawaii. Spacings tested were 8 by 8 feet (2.4 m), 10 by 10 feet (3.0 m), 12 by 12 feet (3.7 m), and 14 by 14 feet (4.3 m). Plot trees were measured at ages 1, 2,5, 10, and 15 years...

Optimal management and productivity of Eucalyptus grandis on former phosphate mined and citrus lands in central and southern Florida: influence of genetics and spacing

Treesearch

Kyle W. Fabbro; Donald L. Rockwood

2016-01-01

Eucalyptus short rotation woody crops (SRWC) with superior genotypes are promising in central and south Florida due to their fast growth, freeze resilience, coppicing ability, and site tolerance. Four Eucalyptus grandis cultivars, E.nergy™ G1, G2, G3, and/or G5, were established in 2009 at varying planting densities on a...

Shifts in the bacterial community composition along deep soil profiles in monospecific and mixed stands of Eucalyptus grandis and Acacia mangium.

PubMed

Pereira, Arthur Prudêncio de Araujo; Andrade, Pedro Avelino Maia de; Bini, Daniel; Durrer, Ademir; Robin, Agnès; Bouillet, Jean Pierre; Andreote, Fernando Dini; Cardoso, Elke Jurandy Bran Nogueira

2017-01-01

Our knowledge of the rhizosphere bacterial communities in deep soils and the role of Eucalyptus and Acacia on the structure of these communities remains very limited. In this study, we targeted the bacterial community along a depth profile (0 to 800 cm) and compared community structure in monospecific or mixed plantations of Acacia mangium and Eucalyptus grandis. We applied quantitative PCR (qPCR) and sequence the V6 region of the 16S rRNA gene to characterize composition of bacterial communities. We identified a decrease in bacterial abundance with soil depth, and differences in community patterns between monospecific and mixed cultivations. Sequence analysis indicated a prevalent effect of soil depth on bacterial communities in the mixed plant cultivation system, and a remarkable differentiation of bacterial communities in areas solely cultivated with Eucalyptus. The groups most influenced by soil depth were Proteobacteria and Acidobacteria (more frequent in samples between 0 and 300 cm). The predominant bacterial groups differentially displayed in the monospecific stands of Eucalyptus were Firmicutes and Proteobacteria. Our results suggest that the addition of an N2-fixing tree in a monospecific cultivation system modulates bacterial community composition even at a great depth. We conclude that co-cultivation systems may represent a key strategy to improve soil resources and to establish more sustainable cultivation of Eucalyptus in Brazil.

Monoterpene engineering in a woody plant Eucalyptus camaldulensis using a limonene synthase cDNA.

PubMed

Ohara, Kazuaki; Matsunaga, Etsuko; Nanto, Kazuya; Yamamoto, Kyoko; Sasaki, Kanako; Ebinuma, Hiroyasu; Yazaki, Kazufumi

2010-01-01

Metabolic engineering aimed at monoterpene production has become an intensive research topic in recent years, although most studies have been limited to herbal plants including model plants such as Arabidopsis. The genus Eucalyptus includes commercially important woody plants in terms of essential oil production and the pulp industry. This study attempted to modify the production of monoterpenes, which are major components of Eucalyptus essential oil, by introducing two expression constructs containing Perilla frutescens limonene synthase (PFLS) cDNA, whose gene products were designed to be localized in either the plastid or cytosol, into Eucalyptus camaldulensis. The expression of the plastid-type and cytosol-type PFLS cDNA in transgenic E. camaldulensis was confirmed by real-time polymerase chain reaction (PCR). Gas chromatography with a flame ionization detector analyses of leaf extracts revealed that the plastidic and cytosolic expression of PFLS yielded 2.6- and 4.5-times more limonene than that accumulated in wild-type E. camaldulensis, respectively, while the ectopic expression of PFLS had only a small effect on the emission of limonene from the leaves of E. camaldulensis. Surprisingly, the high level of PFLS in Eucalyptus was accompanied by a synergistic increase in the production of 1,8-cineole and alpha-pinene, two major components of Eucalyptus monoterpenes. This genetic engineering of monoterpenes demonstrated a new potential for molecular breeding in woody plants.

Control of Tetranychus urticae Koch by extracts of three essential oils of chamomile, marjoram and Eucalyptus.

PubMed

Abd El-Moneim, M R Afify; Fatma, S Ali; Turky, A F

2012-01-01

To evaluate the acaricidal activity of extracts of three essential oils of chamomile, marjoram and Eucalyptus against Tetranychus urticae (T. urticae) Koch. Extracts of three essential oils of chamomile, marjoram and Eucalyptus with different concentrations (0.5%, 1.0%, 2.0%, 3.0% and 4.0%) were used to control T. urticae Koch. The results showed that chamomile (Chamomilla recutita) represented the most potent efficient acaricidal agent against Tetranychus followed by marjoram (Marjorana hortensis) and Eucalyptus. The LC50 values of chamomile, marjoram and Eucalyptus for adults were 0.65, 1.84 and 2.18, respectively and for eggs 1.17, 6.26 and 7.33, respectively. Activities of enzymes including glutathione-S-transferase, esterase (α-esterase and β-esterase) and alkaline phosphatase in susceptible mites were determined and activities of enzymes involved in the resistance of acaricides were proved. Protease enzyme was significantly decreased at LC50 of both chamomile and marjoram compared with positive control. Gas chromatography-mass spectrometer (GC-MS) proved that the major compositions of Chamomilla recutita are α-bisabolol oxide A (35.251%), and trans-β-farersene (7.758%), while the main components of Marjorana hortensis are terpinene-4-ol (23.860%), p-cymene (23.404%) and sabinene (10.904%). It can be concluded that extracts of three essential oils of chamomile, marjoram and Eucalyptus possess acaricidal activity against T. urticae.

Shifts in the bacterial community composition along deep soil profiles in monospecific and mixed stands of Eucalyptus grandis and Acacia mangium

PubMed Central

de Andrade, Pedro Avelino Maia; Bini, Daniel; Durrer, Ademir; Robin, Agnès; Bouillet, Jean Pierre; Andreote, Fernando Dini; Cardoso, Elke Jurandy Bran Nogueira

2017-01-01

Our knowledge of the rhizosphere bacterial communities in deep soils and the role of Eucalyptus and Acacia on the structure of these communities remains very limited. In this study, we targeted the bacterial community along a depth profile (0 to 800 cm) and compared community structure in monospecific or mixed plantations of Acacia mangium and Eucalyptus grandis. We applied quantitative PCR (qPCR) and sequence the V6 region of the 16S rRNA gene to characterize composition of bacterial communities. We identified a decrease in bacterial abundance with soil depth, and differences in community patterns between monospecific and mixed cultivations. Sequence analysis indicated a prevalent effect of soil depth on bacterial communities in the mixed plant cultivation system, and a remarkable differentiation of bacterial communities in areas solely cultivated with Eucalyptus. The groups most influenced by soil depth were Proteobacteria and Acidobacteria (more frequent in samples between 0 and 300 cm). The predominant bacterial groups differentially displayed in the monospecific stands of Eucalyptus were Firmicutes and Proteobacteria. Our results suggest that the addition of an N2-fixing tree in a monospecific cultivation system modulates bacterial community composition even at a great depth. We conclude that co-cultivation systems may represent a key strategy to improve soil resources and to establish more sustainable cultivation of Eucalyptus in Brazil. PMID:28686690

Oxidizable Phenolic Concentrations Do Not Affect Development and Survival of Paropsis Atomaria Larvae Eating Eucalyptus Foliage.

PubMed

Marsh, Karen J; Zhou, Wufeng; Wigley, Hannah J; Foley, William J

2017-04-01

Insect folivores can cause extensive damage to plants. However, different plant species, and even individuals within species, can differ in their susceptibility to insect attack. Polyphenols that readily oxidize have recently gained attention as potential defenses against insect folivores. We tested the hypothesis that variation in oxidizable phenolic concentrations in Eucalyptus foliage influences feeding and survival of Paropsis atomaria (Eucalyptus leaf beetle) larvae. First we demonstrated that oxidizable phenolic concentrations vary both within and between Eucalyptus species, ranging from 0 to 61 mg.g -1 DM (0 to 81% of total phenolics), in 175 samples representing 13 Eucalyptus species. Foliage from six individuals from each of ten species of Eucalyptus were then offered to batches of newly hatched P. atomaria larvae, and feeding, instar progression and mortality of the first and second instar larvae were recorded. Although feeding and survival parameters differed dramatically between individual plants, they were not influenced by the oxidizable phenolic concentration of leaves, suggesting that P. atomaria larvae may have effective mechanisms to deal with oxidizable phenolics. Larvae feeding on plants with higher nitrogen (N) concentrations had higher survival rates and reached third instar earlier, but N concentrations did not explain most of the variation in feeding and survival. The cause of variation in eucalypt herbivory by P. atomaria larvae is therefore still unknown, although oxidizable phenolics could potentially defend eucalypt foliage against other insect herbivores.

Effect of freezing, hot tumble drying and washing with eucalyptus oil on house dust mites in soft toys.

PubMed

Chang, Chin-Fu; Wu, Francis Fu-Sheng; Chen, Chi-Ying; Crane, Julian; Siebers, Rob

2011-09-01

Soft toys are a major source of house dust mites (HDM) and HDM allergens, and sleeping with soft toys is a significant risk factor for HDM sensitization. We studied three techniques to eliminate HDM from soft toys, namely freezing, hot tumble drying and washing with eucalyptus oil. Thirty-six toys (12 in each treatment group) were enumerated for live HDM by the heat escape method before and after freezing overnight, hot tumble drying for 1 h and washing in 0.2% to 0.4% eucalyptus oil. Freezing, hot tumble drying and washing with eucalyptus oil resulted in significant reductions in live HDM, an average reduction of 95.1%, 89.1% and 95.1%, respectively. Additionally, washing with eucalyptus oil resulted in a significant reduction in HDM allergens as well from a geometric mean of 9.12 μg/g to 0.37 μg/g (p = 0.033). These three HDM elimination techniques give parents of infants effective and acceptable methods of limiting HDM exposure. © 2011 John Wiley & Sons A/S.

Antifungal Activity of Eucalyptus Oil against Rice Blast Fungi and the Possible Mechanism of Gene Expression Pattern.

PubMed

Zhou, Li-Jun; Li, Fu-Rong; Huang, Li-Jie; Yang, Zhi-Rong; Yuan, Shu; Bai, Lin-Han

2016-05-12

Eucalyptus oil possesses a wide spectrum of biological activity, including anti-microbial, fungicidal, herbicidal, acaricidal and nematicidal properties. We studied anti-fungal activities of the leaf oil extracted from Eucalyptus. grandis × E. urophylla. Eleven plant pathogenic fungi were tested based on the mycelium growth rates with negative control. The results showed that Eucalyptus oil has broad-spectrum inhibitory effects toward these fungi. Remarkable morphological and structural alterations of hypha have been observed for Magnaporthe grisea after the treatment. The mRNA genome array of M. grisea was used to detect genes that were differentially expressed in the test strains treated by the Eucalyptus oil than the normal strains. The results showed 1919 genes were significantly affected, among which 1109 were down-regulated and 810 were up-regulated (p < 0.05, absolute fold change >2). According to gene ontology annotation analysis, these differentially expressed genes may cause abnormal structures and physiological function disorders, which may reduce the fungus growth. These results show the oil has potential for use in the biological control of plant disease as a green biopesticide.

Pretreatment of eucalyptus with recycled ionic liquids for low-cost biorefinery.

PubMed

Xu, Jikun; Liu, Bingchuan; Hou, Huijie; Hu, Jingping

2017-06-01

It is urgent to develop recycled ionic liquids (ILs) as green solvents for sustainable biomass pretreatment. The goal of this study is to explore the availability and performance of reusing 1-allyl-3-methylimidazolium chloride ([amim]Cl) and 1-butyl-3-methylimidazolium acetate ([bmim]OAc) for pretreatment, structural evolution, and enzymatic hydrolysis of eucalyptus. Cellulose enzymatic digestibility slightly decreased with the increased number of pretreatment recycles. The hydrolysis efficiencies of eucalyptus pretreated via 4th recycled ILs were 54.3% for [amim]Cl and 72.8% for [bmim]OAc, which were 5.0 and 6.7-folds higher than that of untreated eucalyptus. Deteriorations of ILs were observed by the relatively lower sugar conversion and lignin removal from eucalyptus after 4th reuse. No appreciable changes in fundamental framework and thermal stability of [amim]Cl were observed even after successive pretreatments, whereas the anionic structure of [bmim]OAc was destroyed or replaced. This study suggested that the biomass pretreatment with recycled ILs was a potential alternative for low-cost biorefinery. Copyright © 2017 Elsevier Ltd. All rights reserved.

Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection

PubMed Central

Ye, Xiaozhen; Liu, Hongyi; Jin, Yajie; Guo, Mengmeng; Huang, Aizhen; Chen, Quanzhu; Guo, Wenshuo; Zhang, Feiping; Feng, Lizhen

2017-01-01

Eucalyptus leaf blight caused by Calonectria spp. is a serious disease in Eucalyptus seedling and plantations. However, the molecular mechanisms of the infection process and pathogenesis of Calonectria to Eucalyptus is not well-studied. In this study, we analyzed the transcriptomes of C. pseudoreteaudii at three stages of Eucalyptus leaf infection, and in mycelium grown in potato dextrose broth using Illumina RNA-Seq technology. We identified 161 differentially expressed genes between C. pseudoreteaudii from leaf and mycelium grown in potato dextrose broth. GO and KEGG enrichment analyses of these genes suggested that they were mainly involved in oxidoreductase activity, hydrolase activity, and transmembrane transporter activity. Most of the differentially expressed genes at the early infection stage were upregulated. These upregulated genes were mainly involved in cell wall hydrolysis and toxin synthesis, suggesting a role for toxin and cell wall hydrolases in the establishment of Calonectria leaf blight. Genes related to detoxification of phytoalexins were continually upregulated during infection. The candidate effectors and putative pathogenicity determinants identified in this study will help in the functional analysis of C. pseudoreteaudii virulence and pathogenicity. PMID:28072879

Heartwood and sapwood in eucalyptus trees: non-conventional approach to wood quality.

PubMed

Cherelli, Sabrina G; Sartori, Maria Márcia P; Próspero, André G; Ballarin, Adriano W

2018-01-01

This study evaluated the quality of heartwood and sapwood from mature trees of three species of Eucalyptus, by means of the qualification of their proportion, determination of basic and apparent density using non-destructive attenuation of gamma radiation technique and calculation of the density uniformity index. Six trees of each species (Eucalyptus grandis - 18 years old, Eucalyptus tereticornis - 35 years old and Corymbia citriodora - 28 years old) were used in the experimental program. The heartwood and sapwood were delimited by macroscopic analysis and the calculation of areas and percentage of heartwood and sapwood were performed using digital image. The uniformity index was calculated following methodology which numerically quantifies the dispersion of punctual density values of the wood around the mean density along the radius. The percentage of the heartwood was higher than the sapwood in all species studied. The density results showed no statistical difference between heartwood and sapwood. Differently from the density results, in all species studied there was statistical differences between uniformity indexes for heartwood and sapwood regions, making justifiable the inclusion of the density uniformity index as a quality parameter for Eucalyptus wood.

Chemotyping of diverse Eucalyptus species grown in Egypt and antioxidant and antibacterial activities of its respective essential oils.

PubMed

Salem, Mohamed Z M; Ashmawy, Nader A; Elansary, Hosam O; El-Settawy, Ahmed A

2015-01-01

The chemical composition of the essential oil from the leaves of Eucalyptus camaldulensis, Eucalyptus camaldulensis var. obtusa and Eucalyptus gomphocephala grown in northern Egypt was analysed by using GC-FID and GC-MS techniques. The antibacterial (agar disc diffusion and minimum inhibitory concentration methods) and antioxidant activities (2,2'-diphenypicrylhydrazyl) were examined. The main oils constituents were 1,8-cineole (21.75%), β-pinene (20.51%) and methyleugenol (6.10%) in E. camaldulensis; spathulenol (37.46%), p-cymene (17.20%) and crypton (8.88%) in E. gomphocephala; spathulenol (18.37%), p-cymene (19.38%) and crypton (16.91%) in E. camaldulensis var. obtusa. The essential oils from the leaves of Eucalyptus spp. exhibited considerable antibacterial activity against Gram-positive and Gram-negative bacteria. The values of total antioxidant activity were 70 ± 3.13%, 50 ± 3.34% and 84 ± 4.64% for E. camaldulensis, E. camaldulensis var. obtusa and E. gomphocephala, respectively. The highest antioxidant activity value of 84 ± 4.64% could be attributed to the high amount of spathulenol (37.46%).

Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection.

PubMed

Ye, Xiaozhen; Liu, Hongyi; Jin, Yajie; Guo, Mengmeng; Huang, Aizhen; Chen, Quanzhu; Guo, Wenshuo; Zhang, Feiping; Feng, Lizhen

2017-01-01

Eucalyptus leaf blight caused by Calonectria spp. is a serious disease in Eucalyptus seedling and plantations. However, the molecular mechanisms of the infection process and pathogenesis of Calonectria to Eucalyptus is not well-studied. In this study, we analyzed the transcriptomes of C. pseudoreteaudii at three stages of Eucalyptus leaf infection, and in mycelium grown in potato dextrose broth using Illumina RNA-Seq technology. We identified 161 differentially expressed genes between C. pseudoreteaudii from leaf and mycelium grown in potato dextrose broth. GO and KEGG enrichment analyses of these genes suggested that they were mainly involved in oxidoreductase activity, hydrolase activity, and transmembrane transporter activity. Most of the differentially expressed genes at the early infection stage were upregulated. These upregulated genes were mainly involved in cell wall hydrolysis and toxin synthesis, suggesting a role for toxin and cell wall hydrolases in the establishment of Calonectria leaf blight. Genes related to detoxification of phytoalexins were continually upregulated during infection. The candidate effectors and putative pathogenicity determinants identified in this study will help in the functional analysis of C. pseudoreteaudii virulence and pathogenicity.

Contextual view showing northeastern eucalyptus windbreak and portion of citrus ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Contextual view showing northeastern eucalyptus windbreak and portion of citrus orchard. Camera facing 118" east-southeast. - Goerlitz House, 9893 Highland Avenue, Rancho Cucamonga, San Bernardino County, CA

Southern California trial plantings of Eucalyptus

Treesearch

Paul W. Moore

1983-01-01

A trial planting to compare the biomass production of 9 tree species was established in May 1979. Species compared were Acacia melanoxylon, Casuarina cunninghamiana, Casuarina equisitifolia, Eucalyptus camaldulensis, E. dalrympleana, E...

Wood CO2 efflux and foliar respiration for Eucalyptus in Hawaii and Brazil

Treesearch

Michael G. Ryan; Molly A. Cavaleri; Auro C. Almeida; Ricardo Penchel; Randy S. Senock; Jose Luiz Stape

2009-01-01

We measured CO2 efflux from wood for Eucalyptus in Hawaii for 7 years and compared these measurements with those on three- and four-and-a-halfyear- old Eucalyptus in Brazil. In Hawaii, CO2 efflux from wood per unit biomass declined ~10x from age two to age five, twice as much as the decline in tree growth. The CO2 efflux from wood in Brazil was 8-10· lower than that...

Combined effect of pulse density and grid cell size on predicting and mapping aboveground carbon in fast‑growing Eucalyptus forest plantation using airborne LiDAR data

Treesearch

Carlos Alberto Silva; Andrew Thomas Hudak; Carine Klauberg; Lee Alexandre Vierling; Carlos Gonzalez‑Benecke; Samuel de Padua Chaves Carvalho; Luiz Carlos Estraviz Rodriguez; Adrian Cardil

2017-01-01

LiDAR measurements can be used to predict and map AGC across variable-age Eucalyptus plantations with adequate levels of precision and accuracy using 5 pulses m− 2 and a grid cell size of 5 m. The promising results for AGC modeling in this study will allow for greater confidence in comparing AGC estimates with varying LiDAR sampling densities for Eucalyptus plantations...

A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus

PubMed Central

Brondani, Rosana PV; Williams, Emlyn R; Brondani, Claudio; Grattapaglia, Dario

2006-01-01

Background Eucalypts are the most widely planted hardwood trees in the world occupying globally more than 18 million hectares as an important source of carbon neutral renewable energy and raw material for pulp, paper and solid wood. Quantitative Trait Loci (QTLs) in Eucalyptus have been localized on pedigree-specific RAPD or AFLP maps seriously limiting the value of such QTL mapping efforts for molecular breeding. The availability of a genus-wide genetic map with transferable microsatellite markers has become a must for the effective advancement of genomic undertakings. This report describes the development of a novel set of 230 EMBRA microsatellites, the construction of the first comprehensive microsatellite-based consensus linkage map for Eucalyptus and the consolidation of existing linkage information for other microsatellites and candidate genes mapped in other species of the genus. Results The consensus map covers ~90% of the recombining genome of Eucalyptus, involves 234 mapped EMBRA loci on 11 linkage groups, an observed length of 1,568 cM and a mean distance between markers of 8.4 cM. A compilation of all microsatellite linkage information published in Eucalyptus allowed us to establish the homology among linkage groups between this consensus map and other maps published for E. globulus. Comparative mapping analyses also resulted in the linkage group assignment of other 41 microsatellites derived from other Eucalyptus species as well as candidate genes and QTLs for wood and flowering traits published in the literature. This report significantly increases the availability of microsatellite markers and mapping information for species of Eucalyptus and corroborates the high conservation of microsatellite flanking sequences and locus ordering between species of the genus. Conclusion This work represents an important step forward for Eucalyptus comparative genomics, opening stimulating perspectives for evolutionary studies and molecular breeding applications. The generalized use of an increasingly larger set of interspecific transferable markers and consensus mapping information, will allow faster and more detailed investigations of QTL synteny among species, validation of expression-QTL across variable genetic backgrounds and positioning of a growing number of candidate genes co-localized with QTLs, to be tested in association mapping experiments. PMID:16995939

Dynamic-mechanical and thermomechanical properties of cellulose nanofiber/polyester resin composites.

PubMed

Lavoratti, Alessandra; Scienza, Lisete Cristine; Zattera, Ademir José

2016-01-20

Composites of unsaturated polyester resin (UPR) and cellulose nanofibers (CNFs) obtained from dry cellulose waste of softwood (Pinus sp.) and hardwood (Eucalyptus sp.) were developed. The fiber properties and the influence of the CNFs in the dynamic-mechanical and thermomechanical properties of the composites were evaluated. CNFs with a diameter of 70-90 nm were obtained. Eucalyptus sp. has higher α-cellulose content than Pinus sp. fibers. The crystallinity of the cellulose pulps decreased after grinding. However, high values were still obtained. The chemical composition of the fibers was not significantly altered by the grinding process. Eucalyptus sp. CNF composites had water absorption close to the neat resin at 1 wt% filler. The dynamic-mechanical properties of Eucalyptus sp. CNFs were slightly increased and the thermal stability was improved. Copyright © 2015 Elsevier Ltd. All rights reserved.

[Changes of soil nutrient contents after prescribed burning of forestland in Heshan City, Guangdong Province].

PubMed

Sun, Yu-xin; Wu, Jian-ping; Zhou, Li-xia; Lin, Yong-biao; Fu, Sheng-lei

2009-03-01

A comparative study was conducted to analyze the changes of soil nutrient contents in Eucalyptus forestland and in shrubland after three years of prescribed burning. In Eucalyptus forestland, soil organic carbon, total nitrogen, available potassium contents and soil pH decreased significantly; soil available phosphorus and exchangeable magnesium contents, net nitrogen mineralization rate and ammonification rate also decreased but showed no significant difference. In shrubland, soil exchangeable calcium content increased significantly, but the contents of other nutrients had no significant change. The main reason of the lower soil net nitrogen mineralization rate in Eucalyptus forest could be the decrease of available substrates and the uptake of larger amount of soil nutrients by the fast growth of Eucalyptus. The soil nutrients in shrubland had a quick restoration rate after burning.

Control of Tetranychus urticae Koch by extracts of three essential oils of chamomile, marjoram and Eucalyptus

PubMed Central

Abd El-Moneim, MR Afify; Fatma, S Ali; Turky, AF

2012-01-01

Objective To evaluate the acaricidal activity of extracts of three essential oils of chamomile, marjoram and Eucalyptus against Tetranychus urticae (T. urticae) Koch. Methods Extracts of three essential oils of chamomile, marjoram and Eucalyptus with different concentrations (0.5%, 1.0%, 2.0%, 3.0% and 4.0%) were used to control T. urticae Koch. Results The results showed that chamomile (Chamomilla recutita) represented the most potent efficient acaricidal agent against Tetranychus followed by marjoram (Marjorana hortensis) and Eucalyptus. The LC50 values of chamomile, marjoram and Eucalyptus for adults were 0.65, 1.84 and 2.18, respectively and for eggs 1.17, 6.26 and 7.33, respectively. Activities of enzymes including glutathione-S-transferase, esterase (α-esterase and β-esterase) and alkaline phosphatase in susceptible mites were determined and activities of enzymes involved in the resistance of acaricides were proved. Protease enzyme was significantly decreased at LC50 of both chamomile and marjoram compared with positive control. Gas chromatography-mass spectrometer (GC-MS) proved that the major compositions of Chamomilla recutita are α-bisabolol oxide A (35.251%), and trans-β-farersene (7.758%), while the main components of Marjorana hortensis are terpinene-4-ol (23.860%), p-cymene (23.404%) and sabinene (10.904%). Conclusions It can be concluded that extracts of three essential oils of chamomile, marjoram and Eucalyptus possess acaricidal activity against T. urticae. PMID:23569829

Control of key pecan insect pests using biorational pesticides.

PubMed

Shapiro-Ilan, David I; Cottrell, Ted E; Jackson, Mark A; Wood, Bruce W

2013-02-01

Key pecan insect pests include the black pecan aphid, Melanocallis caryaefoliae (Davis), pecan weevil, Curculio caryae (Horn), and stink bugs (Hemiptera: Pentatomidae). Alternative control tactics are needed for management of these pests in organic and conventional systems. Our objective was to evaluate the potential utility of several alternative insecticides including three plant extract formulations, eucalyptus extract, citrus extract-8.92%, and citrus extract-19.4%, and two microbial insecticides, Chromobacterium subtsugae (Martin et al.) and Isaria fumosorosea (Wize). In the laboratory, eucalyptus extract, citrus extract-8.92%, citrus extract-19.4%, and C. subtsugae caused M. caryaefoliae mortality (mortality was reached approximately 78, 83, and 96%, respectively). In field tests, combined applications of I. fumosorosea with eucalyptus extract were synergistic and caused up to 82% mortality in M. caryaefoliae. In laboratory assays focusing on C. caryae suppression, C. subtsugae reduced feeding and oviposition damage, eucalyptus extract and citrus extract-19.4% were ineffective, and antagonism was observed when citrus extract-19.4% was combined with the entomopathogenic nematode, Steinernema carpocapsae (Weiser). In field tests, C. subtsugae reduced C. caryae damage by 55% within the first 3d, and caused 74.5% corrected mortality within 7 d posttreatment. In the laboratory, C. subtsugae and eucalyptus extract did not cause mortality in the brown stink bug, Euschistus servus (Say). Applications of C. subtsugae for suppression of C. caryae, and eucalyptus extract plus I. fumosorosea for control of M. caryaefoliae show promise as alternative insecticides and should be evaluated further.

Flavonoid supplementation affects the expression of genes involved in cell wall formation and lignification metabolism and increases sugar content and saccharification in the fast-growing eucalyptus hybrid E. urophylla x E. grandis.

PubMed

Lepikson-Neto, Jorge; Nascimento, Leandro C; Salazar, Marcela M; Camargo, Eduardo L O; Cairo, João P F; Teixeira, Paulo J; Marques, Wesley L; Squina, Fabio M; Mieczkowski, Piotr; Deckmann, Ana C; Pereira, Gonçalo A G

2014-11-19

Eucalyptus species are the most widely planted hardwood species in the world and are renowned for their rapid growth and adaptability. In Brazil, one of the most widely grown Eucalyptus cultivars is the fast-growing Eucalyptus urophylla x Eucalyptus grandis hybrid. In a previous study, we described a chemical characterization of these hybrids when subjected to flavonoid supplementation on 2 distinct timetables, and our results revealed marked differences between the wood composition of the treated and untreated trees. In this work, we report the transcriptional responses occurring in these trees that may be related to the observed chemical differences. Gene expression was analysed through mRNA-sequencing, and notably, compared to control trees, the treated trees display differential down-regulation of cell wall formation pathways such as phenylpropanoid metabolism as well as differential expression of genes involved in sucrose, starch and minor CHO metabolism and genes that play a role in several stress and environmental responses. We also performed enzymatic hydrolysis of wood samples from the different treatments, and the results indicated higher sugar contents and glucose yields in the flavonoid-treated plants. Our results further illustrate the potential use of flavonoids as a nutritional complement for modifying Eucalyptus wood, since, supplementation with flavonoids alters its chemical composition, gene expression and increases saccharification probably as part of a stress response.

Path analysis of the energy density of wood in eucalyptus clones.

PubMed

Couto, A M; Teodoro, P E; Trugilho, P F

2017-03-16

Path analysis has been used for establishing selection criteria in genetic breeding programs for several crops. However, it has not been used in eucalyptus breeding programs yet. In the present study, we aimed to identify the wood technology traits that could be used as the criteria for direct and indirect selection of eucalyptus genotypes with high energy density of wood. Twenty-four eucalyptus clones were evaluated in a completely randomized design with five replications. The following traits were assessed: basic wood density, total extractives, lignin content, ash content, nitrogen content, carbon content, hydrogen content, sulfur content, oxygen content, higher calorific power, holocellulose, and energy density. After verifying the variability of all evaluated traits among the clones, a two-dimensional correlation network was used to determine the phenotypic patterns among them. The obtained coefficient of determination (0.94) presented a higher magnitude in relation to the effect of the residual variable, and it served as an excellent model for explaining the genetic effects related to the variations observed in the energy density of wood in all eucalyptus clones. However, for future studies, we recommend evaluating other traits, especially the morphological traits, because of the greater ease in their measurement. Selecting clones with high basic density is the most promising strategy for eucalyptus breeding programs that aim to increase the energy density of wood because of its high heritability and magnitude of the cause-and-effect relationship with this trait.

Phylogeny Explains Variation in The Root Chemistry of Eucalyptus Species.

PubMed

Senior, John K; Potts, Brad M; Davies, Noel W; Wooliver, Rachel C; Schweitzer, Jennifer A; Bailey, Joseph K; O'Reilly-Wapstra, Julianne M

2016-10-01

Plants are dependent on their root systems for survival, and thus are defended from belowground enemies by a range of strategies, including plant secondary metabolites (PSMs). These compounds vary among species, and an understanding of this variation may provide generality in predicting the susceptibility of forest trees to belowground enemies and the quality of their organic matter input to soil. Here, we investigated phylogenetic patterns in the root chemistry of species within the genus Eucalyptus. Given the known diversity of PSMs in eucalypt foliage, we hypothesized that (i) the range and concentrations of PSMs and carbohydrates in roots vary among Eucalyptus species, and (ii) that phylogenetic relationships explain a significant component of this variation. To test for interspecific variation in root chemistry and the influence of tree phylogeny, we grew 24 Eucalyptus species representing two subgenera (Eucalyptus and Symphyomyrtus) in a common garden for two years. Fine root samples were collected from each species and analyzed for total phenolics, condensed tannins, carbohydrates, terpenes, and formylated phloroglucinol compounds. Compounds displaying significant interspecific variation were mapped onto a molecular phylogeny and tested for phylogenetic signal. Although all targeted groups of compounds were present, we found that phenolics dominated root defenses and that all phenolic traits displayed significant interspecific variation. Further, these compounds displayed a significant phylogenetic signal. Overall, our results suggest that within these representatives of genus Eucalyptus, more closely related species have more similar root chemistry, which may influence their susceptibility to belowground enemies and soil organic matter accrual.

Fungal Planet description sheets: 558-624.

PubMed

Crous, P W; Wingfield, M J; Burgess, T I; Hardy, G E St J; Barber, P A; Alvarado, P; Barnes, C W; Buchanan, P K; Heykoop, M; Moreno, G; Thangavel, R; van der Spuy, S; Barili, A; Barrett, S; Cacciola, S O; Cano-Lira, J F; Crane, C; Decock, C; Gibertoni, T B; Guarro, J; Guevara-Suarez, M; Hubka, V; Kolařík, M; Lira, C R S; Ordoñez, M E; Padamsee, M; Ryvarden, L; Soares, A M; Stchigel, A M; Sutton, D A; Vizzini, A; Weir, B S; Acharya, K; Aloi, F; Baseia, I G; Blanchette, R A; Bordallo, J J; Bratek, Z; Butler, T; Cano-Canals, J; Carlavilla, J R; Chander, J; Cheewangkoon, R; Cruz, R H S F; da Silva, M; Dutta, A K; Ercole, E; Escobio, V; Esteve-Raventós, F; Flores, J A; Gené, J; Góis, J S; Haines, L; Held, B W; Jung, M Horta; Hosaka, K; Jung, T; Jurjević, Ž; Kautman, V; Kautmanova, I; Kiyashko, A A; Kozanek, M; Kubátová, A; Lafourcade, M; La Spada, F; Latha, K P D; Madrid, H; Malysheva, E F; Manimohan, P; Manjón, J L; Martín, M P; Mata, M; Merényi, Z; Morte, A; Nagy, I; Normand, A-C; Paloi, S; Pattison, N; Pawłowska, J; Pereira, O L; Petterson, M E; Picillo, B; Raj, K N A; Roberts, A; Rodríguez, A; Rodríguez-Campo, F J; Romański, M; Ruszkiewicz-Michalska, M; Scanu, B; Schena, L; Semelbauer, M; Sharma, R; Shouche, Y S; Silva, V; Staniaszek-Kik, M; Stielow, J B; Tapia, C; Taylor, P W J; Toome-Heller, M; Vabeikhokhei, J M C; van Diepeningen, A D; Van Hoa, N; M, Van Tri; Wiederhold, N P; Wrzosek, M; Zothanzama, J; Groenewald, J Z

2017-06-01

Novel species of fungi described in this study include those from various countries as follows: Australia : Banksiophoma australiensis (incl. Banksiophoma gen. nov.) on Banksia coccinea , Davidiellomyces australiensis (incl. Davidiellomyces gen. nov.) on Cyperaceae , Didymocyrtis banksiae on Banksia sessilis var . cygnorum , Disculoides calophyllae on Corymbia calophylla , Harknessia banksiae on Banksia sessilis , Harknessia banksiae-repens on Banksia repens , Harknessia banksiigena on Banksia sessilis var . cygnorum , Harknessia communis on Podocarpus sp., Harknessia platyphyllae on Eucalyptus platyphylla , Myrtacremonium eucalypti (incl. Myrtacremonium gen. nov.) on Eucalyptus globulus , Myrtapenidiella balenae on Eucalyptus sp., Myrtapenidiella eucalyptigena on Eucalyptus sp., Myrtapenidiella pleurocarpae on Eucalyptus pleurocarpa , Paraconiothyrium hakeae on Hakea sp., Paraphaeosphaeria xanthorrhoeae on Xanthorrhoea sp., Parateratosphaeria stirlingiae on Stirlingia sp., Perthomyces podocarpi (incl. Perthomyces gen. nov.) on Podocarpus sp., Readeriella ellipsoidea on Eucalyptus sp., Rosellinia australiensis on Banksia grandis , Tiarosporella corymbiae on Corymbia calophylla , Verrucoconiothyrium eucalyptigenum on Eucalyptus sp., Zasmidium commune on Xanthorrhoea sp., and Zasmidium podocarpi on Podocarpus sp. Brazil : Cyathus aurantogriseocarpus on decaying wood, Perenniporia brasiliensis on decayed wood, Perenniporia paraguyanensis on decayed wood, and Pseudocercospora leandrae-fragilis on Leandra fragilis. Chile : Phialocephala cladophialophoroides on human toe nail. Costa Rica : Psathyrella striatoannulata from soil. Czech Republic : Myotisia cremea (incl. Myotisia gen. nov.) on bat droppings. Ecuador : Humidicutis dictiocephala from soil, Hygrocybe macrosiparia from soil, Hygrocybe sangayensis from soil, and Polycephalomyces onorei on stem of Etlingera sp. France : Westerdykella centenaria from soil. Hungary : Tuber magentipunctatum from soil. India : Ganoderma mizoramense on decaying wood, Hodophilus indicus from soil, Keratinophyton turgidum in soil, and Russula arunii on Pterigota alata. Italy : Rhodocybe matesina from soil. Malaysia : Apoharknessia eucalyptorum , Harknessia malayensis , Harknessia pellitae , and Peyronellaea eucalypti on Eucalyptus pellita , Lectera capsici on Capsicum annuum , and Wallrothiella gmelinae on Gmelina arborea. Morocco : Neocordana musigena on Musa sp. New Zealand : Candida rongomai-pounamu on agaric mushroom surface, Candida vespimorsuum on cup fungus surface, Cylindrocladiella vitis on Vitis vinifera , Foliocryphia eucalyptorum on Eucalyptus sp., Ramularia vacciniicola on Vaccinium sp., and Rhodotorula ngohengohe on bird feather surface. Poland : Tolypocladium fumosum on a caterpillar case of unidentified Lepidoptera. Russia : Pholiotina longistipitata among moss. Spain : Coprinopsis pseudomarcescibilis from soil, Eremiomyces innocentii from soil, Gyroporus pseudocyanescens in humus, Inocybe parvicystis in humus, and Penicillium parvofructum from soil. Unknown origin : Paraphoma rhaphiolepidis on Rhaphiolepsis indica. USA : Acidiella americana from wall of a cooling tower, Neodactylaria obpyriformis (incl. Neodactylaria gen. nov.) from human bronchoalveolar lavage, and Saksenaea loutrophoriformis from human eye. Vietnam : Phytophthora mekongensis from Citrus grandis , and Phytophthora prodigiosa from Citrus grandis. Morphological and culture characteristics along with DNA barcodes are provided.

Bioefficacy of some plant derivatives that protect grain against the pulse beetle, Callosobruchus maculatus

PubMed Central

Rahman, A.; Talukder, F. A.

2006-01-01

Experiments were conducted to study the bioefficacies of different plant/weed derivatives that affect the development of the pulse beetle, Callosobruchus maculates F. (Coleoptera: Bruchidae) fed on black gram, Vigna mungo, seeds. Plant extracts, powder, ash and oil from nishinda (Vitex negundo L.), eucalyptus (Eucalyptus globules Labill.), bankalmi (Ipomoea sepiaria K.), neem (Azadirachta indica L.), safflower (Carthamus tinctorius L.), sesame (Sesamum indicum L.) and bablah (Acacia arabica L.) were evaluated for their oviposition inhibition, surface protectant, residual toxicity and direct toxicity effects on C. maculates. The results showed that plant oils were effective in checking insect infestation. The least number of F1 adults emerged from black gram seeds treated with neem oil. The nishinda oil extract was the most toxic of three extracts tested (nishinda, eucalyptus and bankalmi). Bablah ash was the most effective compared to the powdered leaves of nishinda, eucalyptus and bankalmi. The powdered leaves and extracts of nishinda, eucalyptus and bankalmi, at a 3% mixture, provided good protection for black gram seeds by reducing insect oviposition, F1 adult emergence, and grain infestation rates. The oil treatment did not show adverse effects on germination capability of seeds, even after three months of treatment. PMID:19537990

Larvicidal potential of essential oils against Musca domestica and Anopheles stephensi.

PubMed

Chauhan, Nitin; Malik, Anushree; Sharma, Satyawati; Dhiman, R C

2016-06-01

The larvicidal activity of Mentha piperita, Cymbopogan citratus (lemongrass), Eucalyptus globulus and Citrus sinensis (orange) essential oils and their combinations was evaluated against Musca domestica (housefly) and Anopheles stephensi (mosquitoes) through contact toxicity assay. Among all the tested essential oils/combinations, Me. piperita was found to be the most effective larvicidal agent against Mu. domestica and An. stephensi with LC50 values of 0.66 μl/cm(2) and 44.66 ppm, respectively, after 48 h. The results clearly highlighted that the addition of mentha oil to other oils (1:1 ratio) improved their larvicidal activity. The order of effectiveness of essential oils/combinations indicated that the pattern for An. stephensi follows the trend as mentha > mentha + lemongrass > lemongrass > mentha + eucalyptus > eucalyptus > mentha + orange > orange and for Mu. domestica as mentha > mentha + lemongrass > lemongrass > mentha + orange > orange > mentha + eucalyptus > eucalyptus. The images obtained from scanning electron microscopy (SEM) analysis indicated the toxic effect of Me. piperita as the treated larvae were observed to be dehydrated and deformed. This study demonstrates the effectiveness of tested essential oils/combinations against the larval stages of Mu. domestica and An. stephensi and has the potential for development of botanical formulations.

Bioefficacy of some plant derivatives that protect grain against the pulse beetle, Callosobruchus maculatus.

PubMed

Rahman, A; Talukder, F A

2006-01-01

Experiments were conducted to study the bioefficacies of different plant/weed derivatives that affect the development of the pulse beetle, Callosobruchus maculates F. (Coleoptera: Bruchidae) fed on black gram, Vigna mungo, seeds. Plant extracts, powder, ash and oil from nishinda (Vitex negundo L.), eucalyptus (Eucalyptus globules Labill.), bankalmi (Ipomoea sepiaria K.), neem (Azadirachta indica L.), safflower (Carthamus tinctorius L.), sesame (Sesamum indicum L.) and bablah (Acacia arabica L.) were evaluated for their oviposition inhibition, surface protectant, residual toxicity and direct toxicity effects on C. maculates. The results showed that plant oils were effective in checking insect infestation. The least number of F(1) adults emerged from black gram seeds treated with neem oil. The nishinda oil extract was the most toxic of three extracts tested (nishinda, eucalyptus and bankalmi). Bablah ash was the most effective compared to the powdered leaves of nishinda, eucalyptus and bankalmi. The powdered leaves and extracts of nishinda, eucalyptus and bankalmi, at a 3% mixture, provided good protection for black gram seeds by reducing insect oviposition, F(1) adult emergence, and grain infestation rates. The oil treatment did not show adverse effects on germination capability of seeds, even after three months of treatment.

Comparative genomics of Eucalyptus and Corymbia reveals low rates of genome structural rearrangement.

PubMed

Butler, J B; Vaillancourt, R E; Potts, B M; Lee, D J; King, G J; Baten, A; Shepherd, M; Freeman, J S

2017-05-22

Previous studies suggest genome structure is largely conserved between Eucalyptus species. However, it is unknown if this conservation extends to more divergent eucalypt taxa. We performed comparative genomics between the eucalypt genera Eucalyptus and Corymbia. Our results will facilitate transfer of genomic information between these important taxa and provide further insights into the rate of structural change in tree genomes. We constructed three high density linkage maps for two Corymbia species (Corymbia citriodora subsp. variegata and Corymbia torelliana) which were used to compare genome structure between both species and Eucalyptus grandis. Genome structure was highly conserved between the Corymbia species. However, the comparison of Corymbia and E. grandis suggests large (from 1-13 MB) intra-chromosomal rearrangements have occurred on seven of the 11 chromosomes. Most rearrangements were supported through comparisons of the three independent Corymbia maps to the E. grandis genome sequence, and to other independently constructed Eucalyptus linkage maps. These are the first large scale chromosomal rearrangements discovered between eucalypts. Nonetheless, in the general context of plants, the genomic structure of the two genera was remarkably conserved; adding to a growing body of evidence that conservation of genome structure is common amongst woody angiosperms.

Chemical composition and antimicrobial activity of essential oils of Thymus algeriensis, Eucalyptus globulus and Rosmarinus officinalis from Morocco.

PubMed

Ait-Ouazzou, Abdenour; Lorán, Susana; Bakkali, Mohammed; Laglaoui, Amin; Rota, Carmen; Herrera, Antonio; Pagán, Rafael; Conchello, Pilar

2011-11-01

The present study reports on the antimicrobial activity and chemical composition of the essential oils (EOs) of Thymus algeriensis, Eucalyptus globulus and Rosmarinus officinalis from Morocco. The composition of these species was analysed by GC-MS, and 65 components were identified. Eucalyptus globulus EO showed a great similarity with EOs from other regions, with 1,8-cineole (79.85%) the major component. Also rich in this constituent was Rosmarinus officinalis (43.99%). However, the chemical profile of Thymus algeriensis was rather different, and for the first time such a high content of borneol (23.48%) has been described in this EO. The antimicrobial activity of these species has also been studied against seven pathogenic and spoiling bacteria of significant importance. According to the results, Thymus algeriensis showed the best bacteriostatic and bactericidal effect, followed by Eucalyptus globulus and Rosmarinus officinalis. As far as we know this is the first time that minimum inhibitory and bactericidal concentration values have been reported for Eucalyptus globulus EO. Our data support the possible use of this EO as well as Thymus algeriensis EO, as potential natural agents in preservatives for food and pharmaceutical products. Copyright © 2011 Society of Chemical Industry.

Effects of Successive Rotation Regimes on Carbon Stocks in Eucalyptus Plantations in Subtropical China Measured over a Full Rotation.

PubMed

Li, Xiaoqiong; Ye, Duo; Liang, Hongwen; Zhu, Hongguang; Qin, Lin; Zhu, Yuling; Wen, Yuanguang

2015-01-01

Plantations play an important role in carbon sequestration and the global carbon cycle. However, there is a dilemma in that most plantations are managed on short rotations, and the carbon sequestration capacities of these short-rotation plantations remain understudied. Eucalyptus has been widely planted in the tropics and subtropics due to its rapid growth, high adaptability, and large economic return. Eucalyptus plantations are primarily planted in successive rotations with a short rotation length of 6~8 years. In order to estimate the carbon-stock potential of eucalyptus plantations over successive rotations, we chose a first rotation (FR) and a second rotation (SR) stand and monitored the carbon stock dynamics over a full rotation from 1998 to 2005. Our results showed that carbon stock in eucalyptus trees (TC) did not significantly differ between rotations, while understory vegetation (UC) and soil organic matter (SOC) stored less carbon in the SR (1.01 vs. 2.76 Mg.ha(-1) and 70.68 vs. 81.08 Mg. ha(-1), respectively) and forest floor carbon (FFC) conversely stored more (2.80 vs. 2.34 Mg. ha(-1)). The lower UC and SOC stocks in the SR stand resulted in 1.13 times lower overall ecosystem carbon stock. Mineral soils and overstory trees were the two dominant carbon pools in eucalyptus plantations, accounting for 73.77%~75.06% and 20.50%~22.39%, respectively, of the ecosystem carbon pool. However, the relative contribution (to the ecosystem pool) of FFC stocks increased 1.38 times and that of UC decreased 2.30 times in the SR versus FR stand. These carbon pool changes over successive rotations were attributed to intensive successive rotation regimes of eucalyptus plantations. Our eight year study suggests that for the sustainable development of short-rotation plantations, a sound silvicultural strategy is required to achieve the best combination of high wood yield and carbon stock potential.

Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae) Are a Rich Source of Flavonoids and Related Non-Volatile Constituents

PubMed Central

Nicolle, Dean; Woodrow, Ian E.

2016-01-01

The sub-dermal secretory cavities (glands) embedded within the leaves of Eucalyptus (Myrtaceae) were once thought to be the exclusive repositories of monoterpene and sesquiterpene oils. Recent research has debunked this theory and shown that abundant non-volatile compounds also occur within foliar glands. In particular, glands of four species in subgenus Eucalyptus contain the biologically active flavanone pinocembrin. Pinocembrin shows great promise as a pharmaceutical and is predominantly plant-sourced, so Eucalyptus could be a potential commercial source of such compounds. To explore this we quantified and assessed the purity of pinocembrin in glands of 11 species of E. subg. Eucalyptus using Electro-Spray Ionisation Liquid Chromatography Mass Spectrometry of acetonitrile extracts and Gas Chromatography Mass Spectrometry analyses of hexane extracts of isolated glands which were free from other leaf tissues. Our results showed that the glands of subgenus Eucalyptus contain numerous flavanones that are structurally related to pinocembrin and often present in much greater abundance. The maximum concentration of pinocembrin was 2 mg g-1 dry leaf found in E. stellulata, whereas that of dimethylpinocembrin (5,7-dimethoxyflavanone) was 10 mg g-1 in E. oreades and that of pinostrobin (5-hydroxy-7-methoxyflavanone) was 12 mg g-1 in E. nitida. We also found that the flavanones are exclusively located within the foliar glands rather than distributed throughout leaf tissues. The flavanones differ from the non-methylated pinocembrin in the degree and positions of methylation. This finding is particularly important given the attractiveness of methylated flavonoids as pharmaceuticals and therapeutics. Another important finding was that glands of some members of the subgenus also contain flavanone O-glucosides and flavanone-β-triketone conjugates. In addition, glands contain free β-triketones, β-triketone heterodimers and chromone C-glucosides. Therefore, the foliar glands of this taxonomically distinct group of plants are a rich source of a range of flavonoids and other biologically active compounds with great commercial potential. PMID:26977933

Effects of Successive Rotation Regimes on Carbon Stocks in Eucalyptus Plantations in Subtropical China Measured over a Full Rotation

PubMed Central

Li, Xiaoqiong; Ye, Duo; Liang, Hongwen; Zhu, Hongguang; Qin, Lin; Zhu, Yuling; Wen, Yuanguang

2015-01-01

Plantations play an important role in carbon sequestration and the global carbon cycle. However, there is a dilemma in that most plantations are managed on short rotations, and the carbon sequestration capacities of these short-rotation plantations remain understudied. Eucalyptus has been widely planted in the tropics and subtropics due to its rapid growth, high adaptability, and large economic return. Eucalyptus plantations are primarily planted in successive rotations with a short rotation length of 6~8 years. In order to estimate the carbon-stock potential of eucalyptus plantations over successive rotations, we chose a first rotation (FR) and a second rotation (SR) stand and monitored the carbon stock dynamics over a full rotation from 1998 to 2005. Our results showed that carbon stock in eucalyptus trees (TC) did not significantly differ between rotations, while understory vegetation (UC) and soil organic matter (SOC) stored less carbon in the SR (1.01 vs. 2.76 Mg.ha-1 and 70.68 vs. 81.08 Mg. ha-1, respectively) and forest floor carbon (FFC) conversely stored more (2.80 vs. 2.34 Mg. ha-1). The lower UC and SOC stocks in the SR stand resulted in 1.13 times lower overall ecosystem carbon stock. Mineral soils and overstory trees were the two dominant carbon pools in eucalyptus plantations, accounting for 73.77%~75.06% and 20.50%~22.39%, respectively, of the ecosystem carbon pool. However, the relative contribution (to the ecosystem pool) of FFC stocks increased 1.38 times and that of UC decreased 2.30 times in the SR versus FR stand. These carbon pool changes over successive rotations were attributed to intensive successive rotation regimes of eucalyptus plantations. Our eight year study suggests that for the sustainable development of short-rotation plantations, a sound silvicultural strategy is required to achieve the best combination of high wood yield and carbon stock potential. PMID:26186367

Genomic Characterization of DArT Markers Based on High-Density Linkage Analysis and Physical Mapping to the Eucalyptus Genome

PubMed Central

Petroli, César D.; Sansaloni, Carolina P.; Carling, Jason; Steane, Dorothy A.; Vaillancourt, René E.; Myburg, Alexander A.; da Silva, Orzenil Bonfim; Pappas, Georgios Joannis; Kilian, Andrzej; Grattapaglia, Dario

2012-01-01

Diversity Arrays Technology (DArT) provides a robust, high throughput, cost-effective method to query thousands of sequence polymorphisms in a single assay. Despite the extensive use of this genotyping platform for numerous plant species, little is known regarding the sequence attributes and genome-wide distribution of DArT markers. We investigated the genomic properties of the 7,680 DArT marker probes of a Eucalyptus array, by sequencing them, constructing a high density linkage map and carrying out detailed physical mapping analyses to the Eucalyptus grandis reference genome. A consensus linkage map with 2,274 DArT markers anchored to 210 microsatellites and a framework map, with improved support for ordering, displayed extensive collinearity with the genome sequence. Only 1.4 Mbp of the 75 Mbp of still unplaced scaffold sequence was captured by 45 linkage mapped but physically unaligned markers to the 11 main Eucalyptus pseudochromosomes, providing compelling evidence for the quality and completeness of the current Eucalyptus genome assembly. A highly significant correspondence was found between the locations of DArT markers and predicted gene models, while most of the 89 DArT probes unaligned to the genome correspond to sequences likely absent in E. grandis, consistent with the pan-genomic feature of this multi-Eucalyptus species DArT array. These comprehensive linkage-to-physical mapping analyses provide novel data regarding the genomic attributes of DArT markers in plant genomes in general and for Eucalyptus in particular. DArT markers preferentially target the gene space and display a largely homogeneous distribution across the genome, thereby providing superb coverage for mapping and genome-wide applications in breeding and diversity studies. Data reported on these ubiquitous properties of DArT markers will be particularly valuable to researchers working on less-studied crop species who already count on DArT genotyping arrays but for which no reference genome is yet available to allow such detailed characterization. PMID:22984541

Foliar Essential Oil Glands of Eucalyptus Subgenus Eucalyptus (Myrtaceae) Are a Rich Source of Flavonoids and Related Non-Volatile Constituents.

PubMed

Goodger, Jason Q D; Seneratne, Samiddhi L; Nicolle, Dean; Woodrow, Ian E

2016-01-01

The sub-dermal secretory cavities (glands) embedded within the leaves of Eucalyptus (Myrtaceae) were once thought to be the exclusive repositories of monoterpene and sesquiterpene oils. Recent research has debunked this theory and shown that abundant non-volatile compounds also occur within foliar glands. In particular, glands of four species in subgenus Eucalyptus contain the biologically active flavanone pinocembrin. Pinocembrin shows great promise as a pharmaceutical and is predominantly plant-sourced, so Eucalyptus could be a potential commercial source of such compounds. To explore this we quantified and assessed the purity of pinocembrin in glands of 11 species of E. subg. Eucalyptus using Electro-Spray Ionisation Liquid Chromatography Mass Spectrometry of acetonitrile extracts and Gas Chromatography Mass Spectrometry analyses of hexane extracts of isolated glands which were free from other leaf tissues. Our results showed that the glands of subgenus Eucalyptus contain numerous flavanones that are structurally related to pinocembrin and often present in much greater abundance. The maximum concentration of pinocembrin was 2 mg g-1 dry leaf found in E. stellulata, whereas that of dimethylpinocembrin (5,7-dimethoxyflavanone) was 10 mg g-1 in E. oreades and that of pinostrobin (5-hydroxy-7-methoxyflavanone) was 12 mg g-1 in E. nitida. We also found that the flavanones are exclusively located within the foliar glands rather than distributed throughout leaf tissues. The flavanones differ from the non-methylated pinocembrin in the degree and positions of methylation. This finding is particularly important given the attractiveness of methylated flavonoids as pharmaceuticals and therapeutics. Another important finding was that glands of some members of the subgenus also contain flavanone O-glucosides and flavanone-β-triketone conjugates. In addition, glands contain free β-triketones, β-triketone heterodimers and chromone C-glucosides. Therefore, the foliar glands of this taxonomically distinct group of plants are a rich source of a range of flavonoids and other biologically active compounds with great commercial potential.

Eucalyptus pollen allergy and asthma in children: a cross-sectional study in South-East Queensland, Australia.

PubMed

Gibbs, Jane E M

2015-01-01

To investigate Eucalyptus (gum tree) pollen allergy in children in relation to geography, particularly vegetation, and its relationship to asthma. Males (n = 180) and females (n = 200) aged 9 to 14 participated. Some were healthy (asymptomatic), some had asthma, and some had other symptoms associated with atopy. School students were from three urban coastal schools and one school from a nearby semi-rural elevated area (range) near Brisbane, Australia. Coastal and range locations featured different distributions of Myrtaceae family vegetation (including Eucalyptus, Melaleuca, Leptospermum species). Skin prick test (SPT) responses to 15 commercial allergens were compared. As well, responses from coast versus range groups, and 'asthma' (n = 97) versus 'healthy' status (n = 107) groups, were compared. SPT responses (≥3mm wheal diameter) indicate that children with asthma are 31.1 times more likely to be allergic to Eucalyptus pollen extract (OR: 31.1; 95%CI 4.1- 235.7) compared to healthy children. Dust mite (p = .018), Eucalyptus (p = .046) and cockroach (p = .047) allergen SPT responses (wheals ≥3mm) were significantly greater in participants located on the coast versus range as determined by Fisher's Exact Test (α .05). For each location, percentage of positive responses (wheals ≥3mm) was greatest for 'dust mite' (30.9%-46%), 'cockroach' (18.1% -35%) and 'Bermuda grass' (10.6%-19.4%). The results support the hypothesis that proximity to Myrtaceae vegetation is related to positive SPT response and that Eucalyptus is an important allergen for children with asthma. Substantial response to olive allergen, in the absence of olive trees, suggests that the response may be driven by substances in other plants, perhaps Melaleuca quinquenervia, which abounds in coastal areas. Response to Eucalyptus allergen indicates that changes in gardening practice in schools and public areas may be appropriate. The findings pose validity questions regarding the use of some commercial allergens due to cross-reactive responses and the sources of those responses.

Contextual view of Goerlitz Property, showing eucalyptus trees along west ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Contextual view of Goerlitz Property, showing eucalyptus trees along west side of driveway; parking lot and utility pole in foreground. Camera facing 38" northeast - Goerlitz House, 9893 Highland Avenue, Rancho Cucamonga, San Bernardino County, CA

Micropropagation of frost-resistant Eucalyptus

Treesearch

Michel Boulay

1983-01-01

A method for the in vitro propagation of frost resistant eucalyptus is presented. It was used for the propagation of 2-30 years old trees. This method is presently used for the fast production of mother trees from selected trees.

A NEW SPECIES OF INVASIVE GALL WASP (HYMENOPTERA: EULOPHIDAE: TETRASTICHINAE) ON BLUE GUM (EUCALYPTUS GLOBULUS) IN CALIFORNIA

USDA-ARS?s Scientific Manuscript database

The blue gum gall wasp, Selitrichodes globulus La Salle & Gates (Hymenoptera: Eulophidae: Tetrastichinae), is described as an invasive gall inducer on blue gum, Eucalyptus globulus (Myrtaceae), in California....

Eucalyptus microfungi known from culture. 1. Cladoriella and Fulvoflamma genera nova, with notes on some other poorly known taxa

PubMed Central

Crous, Pedro W.; Verkley, Gerard J.M.; Groenewald, Johannes Z.

2006-01-01

A study of microfungi associated with living Eucalyptus leaves and leaf litter revealed several novel and interesting taxa. Cladoriella eucalypti gen. et sp. nov. is described as a Cladosporium-like genus associated with litter collected in South Africa, while Fulvoflamma eucalypti gen. et. sp. nov. is newly described from leaf litter collected in Spain. Beta-conidia are newly reported for species of Pestalotiopsis, namely Pestalotiopsis disseminata in New Zealand, and a Pestalotiopsis sp. from Colombia. Satchmopsis brasiliensis is reported from litter in Colombia and Indonesia, while Torrendiella eucalypti is reported from leaf litter in Indonesia, and shown to have a Sporothrix-like anamorph. Leptospora rubella is reported from living Eucalyptus leaves in Colombia, where it is associated with leaf spots of Mycosphaerella longibasalis, while Macrohilum eucalypti is reported from leaf spots of Eucalyptus in New Zealand. PMID:18490971

Evaluation of reforested areas using LANDSAT imagery

NASA Technical Reports Server (NTRS)

Dejesusparada, N. (Principal Investigator); Filho, P. H.; Shimabukuro, Y. E.

1978-01-01

The author has identified the following significant results. Visual and automatic interpretation of LANDSAT imagery was used to classify the general Pinus and Eucalyptus according to their age and species. A methodology was derived, based on training areas, to define the legend and spectral characteristics of the analyzed classes. Imager analysis of the training areas show that Pinus taeda is separable from the other Pinus species based on JM distance measurement. No difference of JM measurements was observed among Eucalyptus species. Two classes of Eucalyptus were separated according to their ages: those under and those over two years of age. Channel 6 and 7 were suitable for the discrimination of the reforested classes. Channel 5 was efficient to separated reforested areas from nonforested targets in the region. The automatic analysis shows the highest classification precision was obtained for Eucalyptus over two years of age (95.12 percent).

Biological, medicinal and toxicological significance of Eucalyptus leaf essential oil: a review.

PubMed

Dhakad, Ashok K; Pandey, Vijay V; Beg, Sobia; Rawat, Janhvi M; Singh, Avtar

2018-02-01

The genus Eucalyptus L'Heritier comprises about 900 species, of which more than 300 species contain volatile essential oil in their leaves. About 20 species, within these, have a high content of 1,8-cineole (more than 70%), commercially used for the production of essential oils in the pharmaceutical and cosmetic industries. However, Eucalyptus is extensively planted for pulp, plywood and solid wood production, but its leaf aromatic oil has astounding widespread biological activities, including antimicrobial, antiseptic, antioxidant, chemotherapeutic, respiratory and gastrointestinal disorder treatment, wound healing, and insecticidal/insect repellent, herbicidal, acaricidal, nematicidal, and perfumes, soap making and grease remover. In the present review, we have made an attempt to congregate the biological ingredients of leaf essential oil, leaf oil as a natural medicine, and pharmacological and toxicological values of the leaf oil of different Eucalyptus species worldwide. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Nanofibrillated cellulose (CNF) from eucalyptus sawdust as a dry strength agent of unrefined eucalyptus handsheets.

PubMed

Vallejos, María Evangelina; Felissia, Fernando Esteban; Area, María Cristina; Ehman, Nanci Vanesa; Tarrés, Quim; Mutjé, Pere

2016-03-30

Nanofibrillated cellulose has been obtained from the cellulosic fraction of eucalyptus sawdust. The fractionation process involved the partial removal of hemicelluloses and lignin. CNF was obtained using TEMPO oxidation with NaOCl in basic medium followed by mechanical homogenization. The obtained CNF was subsequently used as a dry strength agent on unbleached unrefined eucalyptus pulp. The addition of 3, 6 and 9 wt.% of CNF increased lineally the tensile index of handsheets to about 55 N mg(-1) at 35°SR, compatible with papermachine runnability. The other mechanical properties also increased substantially, and porosity decreased moderately. The estimated specific surface and average diameter of these CNF were 60 m(2)g(-1), and of 41.0 nm, respectively. The addition of 9 wt.% of CNF produced an increase in mechanical strength, equivalent to that produced by PFI refining at 1600 revolutions. Copyright © 2015 Elsevier Ltd. All rights reserved.

The effectiveness of eucalyptus oil, orange oil, and xylene in dissolving different endodontic sealers.

PubMed

Yadav, Hemant Kumar; Yadav, Rakesh Kumar; Chandra, Anil; Thakkar, Rahul Rameshbhai

2016-01-01

The objective of this study was to evaluate the dissolution effectiveness of eucalyptus oil, orange oil, xylene, and distilled water on three different endodontic sealers. About 240 samples of root canal sealers (eighty for each sealer) were prepared and divided into four groups of 20 each for immersion in different organic solvents. Each group was further subdivided into two subgroups (n = 10) for 2 and 10 min of immersion time. The mean percentage of weight loss was determined for each sealer in each solvent at both time periods. Data were statistically analyzed by two factor analysis of variance and significance of mean difference was obtained by Tukey's post hoc test (P < 0.05). The lowest level of solubility was observed for Adseal followed by Apexit Plus and Endomethasone N at both time periods in all solvents. Apexit Plus showed no significant (P > 0.05) difference in its dissolution in all the organic solvents except distilled water at both the time periods. The solubility profile of Endomethasone N and Adseal did not differ significantly among eucalyptus oil, orange oil, and xylene at 2 min and between eucalyptus oil and orange oil at 10 min. However, at 10 min, Endomethasone N and Adseal showed a more pronounced solubility in xylene as compared to both eucalyptus oil and orange oil. In general, xylene was the most effective in dissolving root canal sealers than other organic solvents. Essential oils (eucalyptus oil and orange oil) were found similar in their ability to dissolve Apexit Plus and Endomethasone N.

Ralstonia solanacearum and R. pseudosolanacearum on Eucalyptus: Opportunists or Primary Pathogens?

PubMed Central

Coutinho, Teresa A.; Wingfield, Michael J.

2017-01-01

Ralstonia solanacearum and R. pseudosolanacearum are well known primary pathogens of herbaceous crops. Reports of wilt caused by these pathogens in tree species are limited other than on Eucalyptus species. Despite the widespread occurrence of so-called bacterial wilt on eucalypts in tropical and sub-tropical parts of Africa, Asia, and the Americas, there remain many contradictions relating to the disease. Our field observations over many years in most regions where the disease occurs on Eucalyptus show that it is always associated with trees that have been subjected to severe stress. The disease is typically diagnosed by immersing cut stems in water and observing bacterial streaming, but the identity of the bacteria within this suspension is seldom considered. To add to the confusion, pathogenicity tests on susceptible species or clones are rarely successful. When they do work, they are on small plants in greenhouse trials. It has become all to easy to attribute Eucalyptus death exclusively to Ralstonia infection. Our data strongly suggest that Ralstonia species and probably other bacteria are latent colonists commonly occurring in healthy and particularly clonally propagated eucalypts. The onset of stress factors provide the bacteria with an opportunity to develop. We believe that the resulting stress weakens the defense systems of the trees allowing Ralstonia and bacterial endophytes to proliferate. Overall our research suggests that R. solanacearum and R. pseudosolanacearum are not primary pathogens of Eucalyptus. Short of clear evidence that they are primary pathogens of Eucalyptus it is inappropriate to attribute this disease solely to infection by Ralstonia species. PMID:28553301

Constancy, Distribution, and Frequency of Lepidoptera Defoliators of Eucalyptus grandis and Eucalyptus urophylla (Myrtaceae) in Four Brazilian Regions.

PubMed

Ribeiro, G T; Zanuncio, J C; de S Tavares, W; de S Ramalho, F; Serrão, J E

2016-12-01

The growth of the Brazilian forest sector with monocultures favors the adaptation of Arthropoda pests. The Lepidoptera order includes major pests of Eucalyptus spp. (Myrtaceae). The aim of this work is to study the population constancy, distribution, and frequency of Lepidoptera primary pests of Eucalyptus spp. Lepidoptera pests in Eucalyptus spp. plantations were collected in Três Marias and Guanhães (state of Minas Gerais), Niquelândia (state of Goiás), and Monte Dourado (state of Pará), Brazil, for a period of 5 years, with light traps and captures, every 15 days, for every region. The number of primary pest species (12) has been similar in the four regions, and even with 1.5 to 2.4% of the total species collected, this group has shown a high frequency, especially in Três Marias, Niquelândia, and Monte Dourado, with 66.3, 54.2, and 40.0% of the individuals collected, respectively, for 5 years. The primary pest species have been constant and frequent in all the regions, with population peaks from February to September in Três Marias, February and May in Niquelândia, and from July to September in Monte Dourado. The highest population peaks of these species have been recorded when the Eucalyptus spp. plants are 3 to 6 years old. The Guanhães region is more stable and, therefore, has a lower possibility of outbreaks of the Lepidoptera primary pest species.

21 CFR 310.544 - Drug products containing active ingredients offered over-the-counter (OTC) for use as a smoking...

Code of Federal Regulations, 2011 CFR

2011-04-01

..., eucalyptus oil, ginger (Jamaica), lemon oil (terpeneless), licorice root extract, lobeline (in the form of... such OTC drug product containing cloves, coriander, eucalyptus oil, ginger (Jamaica), lemon oil...

21 CFR 310.544 - Drug products containing active ingredients offered over-the-counter (OTC) for use as a smoking...

Code of Federal Regulations, 2013 CFR

2013-04-01

..., eucalyptus oil, ginger (Jamaica), lemon oil (terpeneless), licorice root extract, lobeline (in the form of... such OTC drug product containing cloves, coriander, eucalyptus oil, ginger (Jamaica), lemon oil...

21 CFR 310.544 - Drug products containing active ingredients offered over-the-counter (OTC) for use as a smoking...

Code of Federal Regulations, 2010 CFR

2010-04-01

..., eucalyptus oil, ginger (Jamaica), lemon oil (terpeneless), licorice root extract, lobeline (in the form of... such OTC drug product containing cloves, coriander, eucalyptus oil, ginger (Jamaica), lemon oil...

21 CFR 310.544 - Drug products containing active ingredients offered over-the-counter (OTC) for use as a smoking...

Code of Federal Regulations, 2014 CFR

2014-04-01

..., eucalyptus oil, ginger (Jamaica), lemon oil (terpeneless), licorice root extract, lobeline (in the form of... such OTC drug product containing cloves, coriander, eucalyptus oil, ginger (Jamaica), lemon oil...

21 CFR 310.544 - Drug products containing active ingredients offered over-the-counter (OTC) for use as a smoking...

Code of Federal Regulations, 2012 CFR

2012-04-01

..., eucalyptus oil, ginger (Jamaica), lemon oil (terpeneless), licorice root extract, lobeline (in the form of... such OTC drug product containing cloves, coriander, eucalyptus oil, ginger (Jamaica), lemon oil...

Phytoremediation of sewage sludge contaminated by trace elements and organic compounds.

PubMed

Guidi Nissim, Werther; Cincinelli, Alessandra; Martellini, Tania; Alvisi, Laura; Palm, Emily; Mancuso, Stefano; Azzarello, Elisa

2018-07-01

Phytoremediation is a green technique being increasingly used worldwide for various purposes including the treatment of municipal sewage sludge (MSS). Most plants proposed for this technique have high nutrient demands, and fertilization is often required to maintain soil fertility and nutrient balance while remediating the substrate. In this context, MSS could be a valuable source of nutrients (especially N and P) and water for plant growth. The aim of this study was to determine the capacity willow (Salix matsudana, cv Levante), poplar (Populus deltoides × Populus nigra, cv Orion), eucalyptus (Eucalyptus camaldulensis) and sunflower (Helianthus annuus) to clean MSS, which is slightly contaminated by trace elements (TEs) and organic pollutants, and to assess their physiological response to this medium. In particular, we aimed to evaluate the TE accumulation by different species as well as the decrease of TEs and organic pollutants in the sludge after one cropping cycle and the effect of MSS on plant growth and physiology. Since MSS did not show any detrimental effect on the biomass yield of any of the species tested, it was found to be a suitable growing medium for these species. TE phytoextraction rates depended on the species, with eucalyptus showing the highest accumulation for Cr, whereas sunflower exhibited the best performance for As, Cu and Zn. At the end of the trial, some TEs (i.e. Cr, Pb and Zn), n-alkanes and PCBs showed a significant concentration decrease in the sludge for all tested species. The highest Cr decrease was observed in pots with eucalyptus (57.4%) and sunflower (53.4%), whereas sunflower showed the highest Cu decrease (44.2%), followed by eucalyptus (41.2%), poplar (16.2%) and willow (14%). A significant decrease (41.1%) of Pb in the eucalyptus was observed. Zn showed a high decrease rate with sunflower (59.5%) and poplar (52%) and to a lesser degree with willow (35.3%) and eucalyptus (25.4%). The highest decrease in n-alkanes concentration in the sludge was found in willow (98.3%) and sunflower (97.3%), whereas eucalyptus has the lowest PCBs concentration (91.8%) in the sludge compared to the beginning of the trial. These results suggest new strategies (e.g. crop rotation and intercropping) to be adopted for a better management of this phytotechnology. Copyright © 2018 Elsevier Inc. All rights reserved.

Influence of carbonization conditions on the pyrolytic carbon deposition in acacia and eucalyptus wood chars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kumar, M.; Gupta, R.C.

1997-04-01

The amount of deposited pyrolytic carbon (resulting from the cracking of volatile matter) was found to depend on wood species and carbonization conditions, such as temperature and heating rate. Maximum pyrolytic carbon deposition in both the acacia and eucalyptus wood chars has been observed at a carbonization temperature of 800 C. Rapid carbonization (higher heating rate) of wood significantly reduces the amount of deposited pyrolytic carbon in resulting chars. Results also indicate that the amount of deposited pyrolytic carbon in acacia wood char is less than that in eucalyptus wood char.

Elastic Cloud Computing Infrastructures in the Open Cirrus Testbed Implemented via Eucalyptus

NASA Astrophysics Data System (ADS)

Baun, Christian; Kunze, Marcel

Cloud computing realizes the advantages and overcomes some restrictionsof the grid computing paradigm. Elastic infrastructures can easily be createdand managed by cloud users. In order to accelerate the research ondata center management and cloud services the OpenCirrusTM researchtestbed has been started by HP, Intel and Yahoo!. Although commercialcloud offerings are proprietary, Open Source solutions exist in the field ofIaaS with Eucalyptus, PaaS with AppScale and at the applications layerwith Hadoop MapReduce. This paper examines the I/O performance ofcloud computing infrastructures implemented with Eucalyptus in contrastto Amazon S3.

Protein domain evolution is associated with reproductive diversification and adaptive radiation in the genus Eucalyptus.

PubMed

Kersting, Anna R; Mizrachi, Eshchar; Bornberg-Bauer, Erich; Myburg, Alexander A

2015-06-01

Eucalyptus is a pivotal genus within the rosid order Myrtales with distinct geographic history and adaptations. Comparative analysis of protein domain evolution in the newly sequenced Eucalyptus grandis genome and other rosid lineages sheds light on the adaptive mechanisms integral to the success of this genus of woody perennials. We reconstructed the ancestral domain content to elucidate the gain, loss and expansion of protein domains and domain arrangements in Eucalyptus in the context of rosid phylogeny. We used functional gene ontology (GO) annotation of genes to investigate the possible biological and evolutionary consequences of protein domain expansion. We found that protein modulation within the angiosperms occurred primarily on the level of expansion of certain domains and arrangements. Using RNA-Seq data from E. grandis, we showed that domain expansions have contributed to tissue-specific expression of tandemly duplicated genes. Our results indicate that tandem duplication of genes, a key feature of the Eucalyptus genome, has played an important role in the expansion of domains, particularly in proteins related to the specialization of reproduction and biotic and abiotic interactions affecting root and floral biology, and that tissue-specific expression of proteins with expanded domains has facilitated subfunctionalization in domain families. © 2014 University of Pretoria New Phytologist © 2014 New Phytologist Trust.

High-resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly.

PubMed

Bartholomé, Jérôme; Mandrou, Eric; Mabiala, André; Jenkins, Jerry; Nabihoudine, Ibouniyamine; Klopp, Christophe; Schmutz, Jeremy; Plomion, Christophe; Gion, Jean-Marc

2015-06-01

Genetic maps are key tools in genetic research as they constitute the framework for many applications, such as quantitative trait locus analysis, and support the assembly of genome sequences. The resequencing of the two parents of a cross between Eucalyptus urophylla and Eucalyptus grandis was used to design a single nucleotide polymorphism (SNP) array of 6000 markers evenly distributed along the E. grandis genome. The genotyping of 1025 offspring enabled the construction of two high-resolution genetic maps containing 1832 and 1773 markers with an average marker interval of 0.45 and 0.5 cM for E. grandis and E. urophylla, respectively. The comparison between genetic maps and the reference genome highlighted 85% of collinear regions. A total of 43 noncollinear regions and 13 nonsynthetic regions were detected and corrected in the new genome assembly. This improved version contains 4943 scaffolds totalling 691.3 Mb of which 88.6% were captured by the 11 chromosomes. The mapping data were also used to investigate the effect of population size and number of markers on linkage mapping accuracy. This study provides the most reliable linkage maps for Eucalyptus and version 2.0 of the E. grandis genome. © 2014 CIRAD. New Phytologist © 2014 New Phytologist Trust.

Tests of 36 Eucalyptus species in northern California

Treesearch

James P. King; Stanley L. Krugmanand

1980-01-01

A trial of 36 species of Eucalyptus near Concord, California, found species of sufficiently rapid growth and good survival to merit further screening. Species from western Australia and of the subgenus Monocalyprus all failed on the site. E. camaldulensis, E. dalrympleana, E....

Lack of association between allozyme heterozygosity and juvenile traits in Eucalyptus

USDA-ARS?s Scientific Manuscript database

Genetic variability for juvenile waits, which included basal diameter, height, biomass accumulation, and growth increment, was studied in eight provenances involving four species, Eucalyptus grandis, E. saligna, E. camaldulensis and E. urophylla, under uniform greenhouse conditions. The species diff...

EUCANEXT: an integrated database for the exploration of genomic and transcriptomic data from Eucalyptus species

PubMed Central

Nascimento, Leandro Costa; Salazar, Marcela Mendes; Lepikson-Neto, Jorge; Camargo, Eduardo Leal Oliveira; Parreiras, Lucas Salera; Carazzolle, Marcelo Falsarella

2017-01-01

Abstract Tree species of the genus Eucalyptus are the most valuable and widely planted hardwoods in the world. Given the economic importance of Eucalyptus trees, much effort has been made towards the generation of specimens with superior forestry properties that can deliver high-quality feedstocks, customized to the industrýs needs for both cellulosic (paper) and lignocellulosic biomass production. In line with these efforts, large sets of molecular data have been generated by several scientific groups, providing invaluable information that can be applied in the development of improved specimens. In order to fully explore the potential of available datasets, the development of a public database that provides integrated access to genomic and transcriptomic data from Eucalyptus is needed. EUCANEXT is a database that analyses and integrates publicly available Eucalyptus molecular data, such as the E. grandis genome assembly and predicted genes, ESTs from several species and digital gene expression from 26 RNA-Seq libraries. The database has been implemented in a Fedora Linux machine running MySQL and Apache, while Perl CGI was used for the web interfaces. EUCANEXT provides a user-friendly web interface for easy access and analysis of publicly available molecular data from Eucalyptus species. This integrated database allows for complex searches by gene name, keyword or sequence similarity and is publicly accessible at http://www.lge.ibi.unicamp.br/eucalyptusdb. Through EUCANEXT, users can perform complex analysis to identify genes related traits of interest using RNA-Seq libraries and tools for differential expression analysis. Moreover, all the bioinformatics pipeline here described, including the database schema and PERL scripts, are readily available and can be applied to any genomic and transcriptomic project, regardless of the organism. Database URL: http://www.lge.ibi.unicamp.br/eucalyptusdb PMID:29220468

Phytotoxic Activity and Chemical Composition of Aqueous Volatile Fractions from Eucalyptus Species

PubMed Central

Zhang, Jinbiao; An, Min; Wu, Hanwen; Liu, De Li; Stanton, Rex

2014-01-01

The essential oils from four Eucalyptus species (E. spathulata, E. salubris, E. brockwayii and E. dundasii) have been previously confirmed to have stronger inhibitory effects on germination and seedling growth of silverleaf nightshade (Solanum elaeagnifolium Cav.). The aqueous volatile fractions (AVFs) were the water soluble volatile fractions produced together with the essential oils (water insoluble fractions) during the steam distillation process. The aim of this study was to further assess the phytotoxicity of AVFs from the four Eucalyptus species and their chemical composition. The fresh leaves of the four Eucalyptus species were used for the extraction of AVFs. The AVFs were tested for their phytotoxic effects on the perennial weed, silverleaf nightshade under laboratory conditions. The chemical compositions of the AVFs were determined by gas chromatograph–mass spectrometry (GC-MS). Our results showed that the AVFs had strong inhibition on the germination and seedling growth of silverleaf nightshade. The inhibition index increased with the increasing concentrations of AVFs. The inhibitory effects of the AVFs varied between different Eucalyptus species. The AVF from E. salubris demonstrated the highest inhibitory activity on the weed tested, with complete inhibition on germination and seedling growth at a concentration of 75%. The GC-MS analysis revealed that 1,8-cineole, isopentyl isovalerate, isomenthol, pinocarvone, trans-pinocarveol, alpha-terpineol and globulol were the main compounds in the AVFs. These results indicated that all AVFs tested had differential inhibition on the germination and seedling growth of silverleaf nightshade, which could be due to the joint effects of compounds present in the AVFs as these compounds were present in different quantities and ratio between Eucalyptus species. PMID:24681490

Eucalyptus as a pollution - controlling energy source

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1995-12-31

This article describes the possibility of using Eucalyptus as a biomass crop in Florida. It is not only a good fuel for energy production, but it can help alleviate the water pollution problem because of its substantial uptake of excess nutrients and tolerance of flooding.

7 CFR 319.37-2 - Prohibited articles.

Code of Federal Regulations, 2010 CFR

2010-01-01

.... (plumegrass) All Puccinia melanocephala H. Syd. & P. Syd. (Sugarcane rust). Eucalyptus spp. (eucalyptus.... (strawberry) not meeting the conditions for importation in § 319.37-5(h) All except Canada Phytophthora... quarantine pests. Poaceae (vegetative parts of all grains and grasses, except species of Bambuseae) All...

Large-scale Eucalyptus energy farms and power cogeneration

Treesearch

Robert C. Noroña

1983-01-01

A thorough evaluation of all factors possibly affecting a large-scale planting of eucalyptus is foremost in determining the cost effectiveness of the planned operation. Seven basic areas of concern must be analyzed:1. Species Selection 2. Site Preparation 3. Planting 4. Weed Control 5....

Copper and lead bioaccumulation by Acacia retinoides and Eucalyptus torquata in sites contaminated as a consequence of extensive ancient mining activities in Cyprus.

PubMed

Pyatt, F B

2001-09-01

Aspects of the industrial archaeology of the northwestern part of the island of Cyprus are outlined. Wastes resultant from copper mining activities of approximately two millennia ago continue to exert an important influence on organisms. Detailed chemical analysis of two tree species growing on archaeologically important metalliferous spoil tips has indicated their ability to bioaccumulate heavy metals and sulfur primarily from the substratum; the bioaccumulation and biomagnification of lead and sulfur are particularly marked in both Acacia and Eucalyptus. The concentrations of elements in different parts of the two tree species are discussed and partitioning is noted together with the fact that while the pod of Acacia and the fruit capsule of Eucalyptus may have an enhanced metal loading, the values in the seeds are much reduced; the importance of this is discussed. The seeds of Acacia differ chemically from those of Eucalyptus. The importance of these plants as biomonitors of environmental quality is noted. Copyright 2001 Academic Press.

Liquid nitrogen pretreatment of eucalyptus sawdust and rice hull for enhanced enzymatic saccharification.

PubMed

Castoldi, Rafael; Correa, Vanesa G; de Morais, Gutierrez Rodrigues; de Souza, Cristina G M; Bracht, Adelar; Peralta, Rosely A; Peralta-Muniz Moreira, Regina F; Peralta, Rosane M

2017-01-01

In this work, liquid nitrogen was used for the first time in the pretreatment of plant biomasses for purposes of enzymatic saccharification. After treatment (cryocrushing), the initial rates of the enzymatic hydrolysis of eucalyptus sawdust and rice hull were increased more than ten-fold. Cryocrushing did not modify significantly the contents of cellulose, hemicellulose and lignin in both eucalyptus sawdust and rice hulls. However, substantial disorganization of the lignocellulosic materials in consequence of the pretreatment could be observed by electron microscopy. Cryocrushing was highly efficient in improving the saccharification of the holocellulose component of the plant biomasses (from 4.3% to 54.1% for eucalyptus sawdust and from 3.9% to 40.6% for rice hull). It is important to emphasize that it consists in a simple operation with low requirements of water and chemicals, no corrosion, no release of products such as soluble phenolics, furfural and hydroxymethylfurfural and no waste generation. Copyright © 2016 Elsevier Ltd. All rights reserved.

Mechanical properties of acacia and eucalyptus wood chars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kumar, M.; Verma, B.B.; Gupta, R.C.

1999-10-01

In the present investigation the effects of carbonization conditions (temperature and heating rate) on the mechanical properties (such as crushing and impact strengths and shatter index) of acacia and eucalyptus wood chars have been determined. The crushing and impact strengths of both the acacia and eucalyptus wood chars (made by slow carbonization) decreased with increase of preparation temperature up to 600 C, followed by an increase thereafter. These wood chars showed a continuous increase in shatter index values with carbonization temperature. In contrast to slow carbonization (heating rate 4 C min{sup {minus}1}), rapid carbonization (heating rate 30 C min{sup {minus}1})more » yielded chars of lower crushing strengths. Slowly carbonized eucalyptus wood gave chars of superior crushing and impact strengths than those produced from acacia wood under the same carbonization conditions. The crushing and impact strengths of these wood chars, in general, have shown an increase with increase in their apparent density. The crushing strength of cubic-shaped wood char decreased with increase in size.« less

Highly organic natural media as permeable reactive barriers: TCE partitioning and anaerobic degradation profile in eucalyptus mulch and compost.

PubMed

Öztürk, Zuhal; Tansel, Berrin; Katsenovich, Yelena; Sukop, Michael; Laha, Shonali

2012-10-01

Batch and column experiments were conducted with eucalyptus mulch and commercial compost to evaluate suitability of highly organic natural media to support anaerobic decomposition of trichloroethylene (TCE) in groundwater. Experimental data for TCE and its dechlorination byproducts were analyzed with Hydrus-1D model to estimate the partitioning and kinetic parameters for the sequential dechlorination reactions during TCE decomposition. The highly organic natural media allowed development of a bioactive zone capable of decomposing TCE under anaerobic conditions. The first order TCE biodecomposition reaction rates were 0.23 and 1.2d(-1) in eucalyptus mulch and compost media, respectively. The retardation factors in the eucalyptus mulch and compost columns for TCE were 35 and 301, respectively. The results showed that natural organic soil amendments can effectively support the anaerobic bioactive zone for remediation of TCE contaminated groundwater. The natural organic media are effective environmentally sustainable materials for use in permeable reactive barriers. Copyright © 2012 Elsevier Ltd. All rights reserved.

Eucalyptus Pollen Allergy and Asthma in Children: A Cross-Sectional Study in South-East Queensland, Australia

PubMed Central

Gibbs, Jane E. M.

2015-01-01

Objectives To investigate Eucalyptus (gum tree) pollen allergy in children in relation to geography, particularly vegetation, and its relationship to asthma. Methods Males (n = 180) and females (n = 200) aged 9 to 14 participated. Some were healthy (asymptomatic), some had asthma, and some had other symptoms associated with atopy. School students were from three urban coastal schools and one school from a nearby semi-rural elevated area (range) near Brisbane, Australia. Coastal and range locations featured different distributions of Myrtaceae family vegetation (including Eucalyptus, Melaleuca, Leptospermum species). Skin prick test (SPT) responses to 15 commercial allergens were compared. As well, responses from coast versus range groups, and ‘asthma’ (n = 97) versus ‘healthy’ status (n = 107) groups, were compared. Results SPT responses (≥3mm wheal diameter) indicate that children with asthma are 31.1 times more likely to be allergic to Eucalyptus pollen extract (OR: 31.1; 95%CI 4.1- 235.7) compared to healthy children. Dust mite (p = .018), Eucalyptus (p = .046) and cockroach (p = .047) allergen SPT responses (wheals ≥3mm) were significantly greater in participants located on the coast versus range as determined by Fisher’s Exact Test (α .05). For each location, percentage of positive responses (wheals ≥3mm) was greatest for ‘dust mite’ (30.9%-46%), ‘cockroach’ (18.1% -35%) and ‘Bermuda grass’ (10.6%-19.4%). Conclusions The results support the hypothesis that proximity to Myrtaceae vegetation is related to positive SPT response and that Eucalyptus is an important allergen for children with asthma. Substantial response to olive allergen, in the absence of olive trees, suggests that the response may be driven by substances in other plants, perhaps Melaleuca quinquenervia, which abounds in coastal areas. Implications Response to Eucalyptus allergen indicates that changes in gardening practice in schools and public areas may be appropriate. The findings pose validity questions regarding the use of some commercial allergens due to cross-reactive responses and the sources of those responses. PMID:25938684

Genome-wide variation in recombination rate in Eucalyptus.

PubMed

Gion, Jean-Marc; Hudson, Corey J; Lesur, Isabelle; Vaillancourt, René E; Potts, Brad M; Freeman, Jules S

2016-08-09

Meiotic recombination is a fundamental evolutionary process. It not only generates diversity, but influences the efficacy of natural selection and genome evolution. There can be significant heterogeneity in recombination rates within and between species, however this variation is not well understood outside of a few model taxa, particularly in forest trees. Eucalypts are forest trees of global economic importance, and dominate many Australian ecosystems. We studied recombination rate in Eucalyptus globulus using genetic linkage maps constructed in 10 unrelated individuals, and markers anchored to the Eucalyptus reference genome. This experimental design provided the replication to study whether recombination rate varied between individuals and chromosomes, and allowed us to study the genomic attributes and population genetic parameters correlated with this variation. Recombination rate varied significantly between individuals (range = 2.71 to 3.51 centimorgans/megabase [cM/Mb]), but was not significantly influenced by sex or cross type (F1 vs. F2). Significant differences in recombination rate between chromosomes were also evident (range = 1.98 to 3.81 cM/Mb), beyond those which were due to variation in chromosome size. Variation in chromosomal recombination rate was significantly correlated with gene density (r = 0.94), GC content (r = 0.90), and the number of tandem duplicated genes (r = -0.72) per chromosome. Notably, chromosome level recombination rate was also negatively correlated with the average genetic diversity across six species from an independent set of samples (r = -0.75). The correlations with genomic attributes are consistent with findings in other taxa, however, the direction of the correlation between diversity and recombination rate is opposite to that commonly observed. We argue this is likely to reflect the interaction of selection and specific genome architecture of Eucalyptus. Interestingly, the differences amongst chromosomes in recombination rates appear stable across Eucalyptus species. Together with the strong correlations between recombination rate and features of the Eucalyptus reference genome, we maintain these findings provide further evidence for a broad conservation of genome architecture across the globally significant lineages of Eucalyptus.

Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis.

PubMed

Du, Pingzhou; Kumar, Manoj; Yao, Yuan; Xie, Qiaoli; Wang, Jinyan; Zhang, Baolong; Gan, Siming; Wang, Yuqi; Wu, Ai-Min

2016-11-24

The Xklp2 (TPX2) proteins belong to the microtubule-associated (MAP) family of proteins. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist with different microtubule functions, for example, maintenance of cell morphology or regulation of cell growth and development. However, the role of members of the TPX family have not been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes. In present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that the genes can be classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins within different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of the genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of the EgMAP20 transcript is the highest in the root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules. Plant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We have performed an initial characterization of the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus.

Enzymatic saccharification of dilute acid pretreated saline crops for fermentable sugar production

USDA-ARS?s Scientific Manuscript database

Four saline crops [athel (Tamarix aphylla L), eucalyptus (Eucalyptus camaldulensis), Jose Tall Wheatgrass (Agropyron elongatum), and Creeping Wild Ryegrass (Leymus triticoides)] that are used in farms for salt uptake from soil and drainage irrigation water have the potential for fuel ethanol product...

Enzymatic saccharization of dilute acid pretreated saline crops for fermentable sugar production

USDA-ARS?s Scientific Manuscript database

Four saline crops [athel (Tamarix aphylla L), eucalyptus (Eucalyptus camaldulensis), Jose Tall Wheatgrass (Agropyron elongatum), and Creeping Wild Ryegrass (Leymus triticoides)] that are used in farms for salt uptake from soil and drainage irrigation water have the potential for fuel ethanol product...

Aggregate Stability of Tropical Soils Under Long-Term Eucalyptus Cultivation

USDA-ARS?s Scientific Manuscript database

Eucalyptus cultivation has increased in all Brazilian regions. Despite the large amount of cultivated area, little is known about how this kind of management system affects soil properties, mainly the aggregate stability. Aggregate stability analyses have proved to be a sensitive tool to measure soi...

Vertically aligned carbon nanotubes from natural precursors by spray pyrolysis method and their field electron emission properties

NASA Astrophysics Data System (ADS)

Ghosh, Pradip; Soga, T.; Tanemura, M.; Zamri, M.; Jimbo, T.; Katoh, R.; Sumiyama, K.

2009-01-01

Vertically aligned carbon nanotubes have been synthesized from botanical hydrocarbons: Turpentine oil and Eucalyptus oil on Si(100) substrate using Fe catalyst by simple spray pyrolysis method at 700°C and at atmospheric pressure. The as-grown carbon nanotubes were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution TEM (HRTEM), thermogravimetric analysis (TGA), differential thermal analysis (DTA), and Raman spectroscopy. It was observed that nanotubes grown from turpentine oil have better degree of graphitization and field emission performance than eucalyptus oil grown carbon nanotubes. The turpentine oil and eucalyptus oil grown carbon nanotubes indicated that the turn-on field of about 1.7 and 1.93 V/μm, respectively, at 10 μA/cm2. The threshold field was observed to be about 2.13 and 2.9 V/μm at 1 mA/cm2 of nanotubes grown from turpentine oil and eucalyptus oil respectively. Moreover, turpentine oil grown carbon nanotubes show higher current density in relative to eucalyptus oil grown carbon nanotubes. The maximum current density of 15.3 mA/cm2 was obtained for ˜3 V/μm corresponding to the nanotubes grown from turpentine oil. The improved field emission performance was attributed to the enhanced crystallinity, fewer defects, and greater length of turpentine oil grown carbon nanotubes.

Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava

PubMed Central

Bini, Andressa Peres; Regiani, Thais; Franceschini, Lívia Maria; Budzinski, Ilara Gabriela Frasson; Marques, Felipe Garbelini; Labate, Mônica Teresa Veneziano; Guidetti-Gonzalez, Simone; Moon, David Henry; Labate, Carlos Alberto

2016-01-01

Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies. PMID:26731728

Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava.

PubMed

Quecine, Maria Carolina; Leite, Thiago Falda; Bini, Andressa Peres; Regiani, Thais; Franceschini, Lívia Maria; Budzinski, Ilara Gabriela Frasson; Marques, Felipe Garbelini; Labate, Mônica Teresa Veneziano; Guidetti-Gonzalez, Simone; Moon, David Henry; Labate, Carlos Alberto

2016-01-01

Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies.

Ecophysiological and foliar nitrogen concentration responses of understorey Acacia spp. and Eucalyptus sp. to prescribed burning.

PubMed

Ma, Ling; Rao, Xingquan; Lu, Ping; Bai, Shahla Hosseini; Xu, Zhihong; Chen, Xiaoyang; Blumfield, Timothy; Xie, Jun

2015-07-01

Eucalyptus spp. is a dominant tree genus in Australia and most Eucalyptus spp. are canopy dominant species. In Australian natural forests, Eucalyptus spp. commonly are associated with understorey legumes which play a crucial role for ecological restoration owing to their nitrogen (N) fixing ability for replenishing the soil N lost after frequent prescribed burning. This study aimed to explore to what extent physiological responses of these species differ 7 and 12 years after last fire. Two most common understorey Acacia spp., Acacia leiocalyx and A. disparrima, as well as one non-leguminous Eucalyptus resinifera, were studied due to their dominance in the forest. Both A. leiocalyx and A. disparrima showed higher carbon (C) assimilation capacity, maximum photosynthetic capacity, and moderate foliar C/N ratio compared with E. resinifera. A. leiocalyx showed various advantages compared to A. disparrima such as higher photosynthetic capacity, adaptation to wider light range and higher foliar total N (TNmass). A. leiocalyx also relied on N2-fixing ability for longer time compared to A. disparrima. The results suggested that the two Acacia spp. were more beneficial to C and N cycles for the post burning ecosystem than the non-N2-fixing species E. resinifera. A. leiocalyx had greater contribution to complementing soil N cycle long after burning compared to A. disparrima.

Insights into temperature modulation of the Eucalyptus globulus and Eucalyptus grandis antioxidant and lignification subproteomes.

PubMed

de Santana Costa, Marília Gabriela; Mazzafera, Paulo; Balbuena, Tiago Santana

2017-05-01

Eucalyptus grandis and Eucalyptus globulus are among the most widely cultivated trees, differing in lignin composition and plantation areas, as E. grandis is mostly cultivated in tropical regions while E. globulus is preferred in temperate areas. As temperature is a key modulator in plant metabolism, a large-scale proteome analysis was carried out to investigate changes in the antioxidant system and the lignification metabolism in plantlets grown at different temperatures. Our strategy allowed the identification of 3111 stem proteins. A total of 103 antioxidant proteins were detected in the stems of both species. Hierarchical clustering revealed that alterations in the antioxidant proteins are more prominent when Eucalyptus seedlings were exposed to high temperature and that the superoxide isoforms coded by the gene Eucgr.B03930 are the most abundant antioxidant enzymes induced by thermal stimulus. Regarding the lignin biosynthesis, our proteomics approach resulted in the identification of 13 of the 17 core proteins involved in this metabolism, corroborating with gene predictions and the proposed lignin toolbox. Quantitative analyses revealed significant differences in 8 protein isoforms, including the ferulate 5-hydroxylase isoform F5H1, a key enzyme in catalyzing the synthesis of sinapyl alcohol, and the cinnamyl alcohol dehydrogenase isoform CAD2, the last enzyme in monolignol biosynthesis. Data are available via ProteomeXchange with identifier PXD005743. Copyright © 2017 Elsevier Ltd. All rights reserved.

Identification of the CAD gene from Eucalyptus urophylla GLU4 and its functional analysis in transgenic tobacco.

PubMed

Chen, B W; Xiao, Y F; Li, J J; Liu, H L; Qin, Z H; Gai, Y; Jiang, X N

2016-12-02

Cinnamyl alcohol dehydrogenase (CAD) catalyzes the final step in lignin biosynthesis. The genus Eucalyptus belongs to the family Myrtaceae, which is the main cultivated species in China. Eucalyptus urophylla GLU4 (GLU4) is widely grown in Guangxi. It is preferred for pulping because of its excellent cellulose content and fiber length. Based on GLU4 and CAD gene expression, a Eucalyptus variety low in lignin content should be obtained using transgenic technology, which could reduce the cost of pulp and improve the pulping rate, and have favorable prospects for application. However, the role and function of CAD in GLU4 is still unclear. In the present study, EuCAD was cloned from GLU4 and identified using bioinformatic tools. Subsequently, in order to evaluate its impact on lignin synthesis, a full-length EuCAD RNAi vector was constructed, and transgenic tobacco was obtained via Agrobacterium-mediated transformation. A significant decrease in CAD expression and lignin content in transgenic tobacco demonstrated a key role for EuCAD in lignin biosynthesis and established a regulatory role for RNAi. In our study, the direct molecular basis of EuCAD expression was determined, and the potential regulatory effects of this RNAi vector on lignin biosynthesis in E. urophylla GLU4 were demonstrated. Our results provide a theoretical basis for the study of lignin biosynthesis in Eucalyptus.

Clonal propagation of eucalyptus in Brazilian nurseries

Treesearch

Ken McNabb; Natal Goncalves; Jose Goncalves

2002-01-01

Brazil has established extensive Eucalyptus plantations to support a growing forest products industry. During the past 25 years, the country has been a pioneer in developing clonal propagation systems to regenerate these highly productive plantations. Original clonal selections optimized disease resistance, coppicing ability, and volume growth, while recent priorities...

Mixed plantations of Eucalyptus and leguminous trees enhance biomass production

Treesearch

Dean S. DeBell; Craig D. Whitesell; Thomas H. Schubert

1985-01-01

Two Eucalyptus species-E. saligna Sm. and E. grandis Hill-are especially favored in Hawaii forwood, fiber, and fuel production because of their quick growth and high yields. Their growth is limited, however, on many sites by low levels of available nitrogen. Supplemental nitrogen can be provided by nitrogen-...

Managing a Coastal Bluegum (Eucalyptus globules) forest

Treesearch

Ralph S. Osterling

1983-01-01

Eucalyptus was thought to be a replacement to oak and other hardwoods for many products. Thousands of acres have been planted and now are in need of management. Management techniques are discussed in context with a coastal stand of approximately 300 acres of mixed aged bluegum. Potential markets are explored.

Predicting volumes in four Hawaii hardwoods...first multivariate equations developed

Treesearch

David A. Sharpnack

1966-01-01

Multivariate regression equations were developed for predicting board-foot (Int. 1/ 4-inch log rule ) and cubic-foot volumes in each 8.15-foot section of trees of four Hawaii hardwood species. The species are koa (Acacia koa), ohia (Metrosideros polymorpha), robusta eucalyptus (Eucalyptus robusta), and...

Developing clones of Eucalyptus cloeziana resistant to rust (Puccinia psidii)

Treesearch

Rafael F. Alfenas; Marcelo M. Coutinho; Camila S. Freitas; Rodrigo G. Freitas; Acelino C. Alfenas

2012-01-01

Besides its high resistance to Chrysoporthe cubensis canker, Eucalyptus cloeziana F. Muell. is a highly valuable tree species for wood production. It can be used for furniture, electric poles, fence posts, and charcoal. Nevertheless, it is highly susceptible to the rust caused by Puccinia psidii, which...

Vegetation management in Eucalyptus

Treesearch

Clyde L. Elmore

1983-01-01

Weeds and weed control are a major problem in the growth and management of Eucalyptus trees. Annual, biennial and perennial weed species are common in sites to be planted. These weeds should be controlled before planting. Preplant, preemergence and postemergence herbicides are discussed. Safe preemergence herbicides include oryzalin, napropamide, oxadiazon, linuron and...

Physiological responses to glyphosate are dependent on Eucalyptus urograndis genotype

USDA-ARS?s Scientific Manuscript database

Two experiments were conducted to evaluate the response of Eucalyptus urograndis genotypes (C219 and GG100) to glyphosate in growth chambers. As glyphosate dose increased (18 up to 720 g ae ha-1), CO2 assimilation rate, transpiration rate, and stomatal conductance decreased fastest and strongest in ...

Polyester composites reinforced with corona-treated fibers from pine, eucalyptus and sugarcane bagasse

USDA-ARS?s Scientific Manuscript database

This study aims to evaluate plant fibers that were surface activated with NaOH and corona discharge before incorporating in ortho unsaturated polyester-based fiber composites. It demonstrates the potential use of lignocellulosic particles, especially eucalyptus that presented the higher values for a...

California wood energy program

Treesearch

Gary Brittner

1983-01-01

Many varieties of eucalyptus adapt well to growing conditions in the coastal and central valley regions of California. The California Department of Forestry is conducting growth research on a variety of sites throughout the state with many species. Eucalyptus is an excellent fuelwood and has potential for other uses, including chemical feedstocks. Plantations...

Solution blow spun nanocomposites of poly(lactic acid)/cellulose nanocrystals from Eucalyptus kraft pulp

USDA-ARS?s Scientific Manuscript database

Cellulose nanocrystals (CNCs) were extracted from Eucalyptus kraft pulp by sulfuric acid hydrolysis, and esterified with maleic anhydride (CNCMA). The incorporation of sulfate ester groups on the cellulose surface resulted in higher stability of the nanoparticles in aqueous suspensions and lower the...

Biomass and nutrient mass of Acacia dealbata and Eucalyptus globulus bioenergy plantations

Treesearch

Timothy J. Albaugh; Rafael A. Rubilar; Chris A. Maier; Eduardo A. Acuña; Rachel L. Cook

2017-01-01

We quantified biomass and nutrient accumulation of Acacia dealbata Link and Eucalyptus globulus Labill. planted at stem densities of 5000 and 15000 ha-1 in a bioenergy plantation in Chile. We tested the hypotheses that species and stocking will not affect biomass or nutrient accumulation. Species and...

Eucalyptus as a landscape tree

Treesearch

W. Douglas Hamilton

1983-01-01

Ninety-two species of Eucalyptus were evaluated at the University of California re- search station in San Jose. The purpose: to find acceptable new street and park trees. Growth rates and horticultural characteristics were noted. Forty-three species were studied in locations statewide to evaluate site adaptation and landscape usefulness; flooded, cold, dry, saline....

Air-drying of Robusta eucalyptus lumber

Treesearch

Roger G. Skolmen

1964-01-01

A study of air-drying 4/4 Eucalyptus robusta lumber in Hilo, Hawaii showed that during typical summer weather it can be dried to below 20 percent moisture content in 2-1/2 months. Grade reduction in 36 percent of the lumber was caused by end splits, insect damage, warp, and surface checking.

Modification of eucalyptus pulp fiber using silane coupling agents with aliphatic side chains of different length

USDA-ARS?s Scientific Manuscript database

The objective of this work was to evaluate the effect of three silane coupling agents with different aliphatic chain lengths on the hydrophobicity of eucalyptus pulp fiber. The three silanes coupling agents used (isobutyltrimethoxysilane, methyltrimethoxysilane, and n-octyltriethoxysilane [OTES]) we...

SCIMITAR: Scalable Stream-Processing for Sensor Information Brokering

DTIC Science & Technology

2013-11-01

IaaS) cloud frameworks including Amazon Web Services and Eucalyptus . For load testing, we used The Grinder [9], a Java load testing framework that...internal Eucalyptus cluster which we could not scale as large as the Amazon environment due to a lack of computation resources. We recreated our

Effect of plant essential oils on Ralstonia solanacearum race 4 causing bacterial wilt of edible ginger

USDA-ARS?s Scientific Manuscript database

Palmarosa (Cymbopogon martini), lemongrass (C. citratus) and eucalyptus (Eucalyptus globulus) oils were investigated for their effects on Ralstonia solanacearum race 4, and their potential use as bio-fumigants for treating pathogen- infested edible ginger (Zingiber officinale R.) fields. Three conce...

Evaluating the reforested area for the municipality of Buri by automatic analysis of LANDSAT imagery. [Sao Paulo, Brazil

NASA Technical Reports Server (NTRS)

Dejesusparada, N. (Principal Investigator); Lee, D. C. L.; Filho, R. H.; Shimabukuro, Y. E.

1979-01-01

The author has identified the following significant results. The class of reforestation (Pinus, Eucalyptus, Araucaria) was defined using iterative image analysis (1-100) and LANDSAT MSS data. Estimates of class area by 1-100 were compared with data supplied by the forestry institute in Sao Paulo. LANDSAT channels 4 and 5 served to differentiate the Pinus, Eucalyptus, and Araucaria from the other trees. Channels 6 and 7 gave best results for differentiating between the classes. A good representative spectral response was obtained for Auraucaria on these two channels. The small relative differences obtained were +4.24% for Araucaria, -7.51% for Pinus, and -32.07% for Eucalyptus.

Optical property analysis of thermally and photolytically aged Eucalyptus Camaldulensis chemithermomechanical pulp (CTMP)

Treesearch

Yao Chen; Yongming Fan; Mandla A. Tshabalala; Nicole M. Stark; Jianmin Gao

2012-01-01

To investigate the optical properties of chemithermomechanical pulp (CTMP) from Eucalyptus camaldulensis, one group of samples of CTMP was aged by heating, and another group was first subjected to bleaching with different bleaching agents, and then aging by exposure to sunlight. Chromophores were analyzed using diffuse reflectance UV-Vis spectra (...

Morphological development of cellulose fibrils of a bleached eucalyptus pulp by mechanical fibrillation

Treesearch

Q.Q. Wang; J.Y. Zhu; R. Gleisner; T.A. Kuster; U. Baxa; S.E. McNeil

2012-01-01

This study reports the production of cellulose nanofibrils (CNF) from a bleached eucalyptus pulp using a commercial stone grinder. Scanning electronic microscopy and transmission electronic microscopy imaging were used to reveal morphological development of CNF at micro and nano scales, respectively. Two major structures were identified (1) highly kinked, naturally...

The Forests of Toro Negro

Treesearch

R.A. Birdsey; D. Jiménez

1985-01-01

The Toro Negro Region of Puerto Rice is 61 percent forested with 20,100 hectares of timberland and 2,300 hectares of other forest land. Eucalyptus robusta accounts for 37 percent of the growing stock volume in the public forest segment. Most eucalyptus plantations in the public forest are read for harvest and regeneration. Private timberland has...

Growth and yield in Eucalyptus globulus

Treesearch

James A. Rinehart; Richard B. Standiford

1983-01-01

A study of the major Eucalyptus globulus stands throughout California conducted by Woodbridge Metcalf in 1924 provides a complete and accurate data set for generating variable site-density yield models. Two models were developed using linear regression techniques. Model I depicts a linear relationship between age and yield best used for stands between five and fifteen...

Production and carbon allocation in a clonal Eucalyptus plantation with water and nutrient manipulations

Treesearch

Jose Luiz Stape; Dan Binkley; Michael G. Ryan

2008-01-01

We examined resource limitations on growth and carbon allocation in a fast-growing, clonal plantation of Eucalyptus grandis urophylla in Brazil by characterizing responses to annual rainfall, and response to irrigation and fertililization for 2 years. Productivity measures included gross primary production (GPP), total belowground carbon allocation (...

Ongoing molecular studies of Eucalyptus powdery mildew in Brazil

Treesearch

N. R. Fonseca; L. M. S. Guimaraes; R. P. Pires; Ned Klopfenstein; M. -S. Kim; A. C. Alfenas

2016-01-01

Powdery mildew diseases are caused by biotrophic fungi in the Erysiphales. These fungal pathogens are easily observed by the whitish powdery appearance caused by their colonization of the aerial surfaces on living plants (Stadnik & Rivera, 2001) (Figure 1). In Brazil, powdery mildew of Eucalyptus spp is increasing under the current nursery production...

Fertilizer Tablets Stimulate Eucalyptus in Florida Trial

Treesearch

George Meskimen

1971-01-01

Eucalyptus plantings in south Florida must grow rapidly to withstand first-year frosts and dominate herbaceous competition. Fertilizer tablets in the planting holes of E. camaldulensis stimulated immediate growth, resulting in stems 50 percent taller and 83 percent thicker than check trees at 6 months. Effects of tablets persisted after 4 years, with...

Production of cellulose nanofibrils from bleached eucalyptus fibers by hyperthermostable endoglucanase treatment and subsequent microfluidization

Treesearch

Wangxia Wang; Michael D. Mozuch; Ronald C. Sabo; Phil Kersten; J.Y. Zhu; Yongcan Jin

2015-01-01

A GH5 hyperthermostable endoglucanase from the archaeon Pyrococcus honkoshii (ph-GH5) and a commercial endoglucanase FR were used to treat bleached eucalyptus pulp (BEP) fibers to produce cellulose nanofibrils (CNFs) through subsequent microfluidization Enzymatic treatments facilitated CNF production due to the reduced degree of polymerization (DP)...

Notes on the occurrence of Oligonychus milleri (McGregor) and oligonychus ununguis (Jacobi) (Acari: Tetranychidae) in Brazil

USDA-ARS?s Scientific Manuscript database

We verified infestation of Oligonychus milleri (McGregor) on plantations of Pinus caribaea (Pinaceae) and of Oligonychus ununguis (Jacobi) on plantations of Eucalyptus urophylla x Eucalyptus grandis (Myrtaceae) in State of Rondônia, Northern region of Brazil. This represents the first record of O. m...

Species trials at the Waiakea Arboretum

Treesearch

Robert E. Burgan; Wesley H. C. Wong Jr.

1971-01-01

Survival, growth, and tree quality of 84 introduced species planted from 1956 to 1960 on the Waiakea Arboretum, near Hilo, Hawaii, were measured in 1970. Four species show good-to-excellent growth and good potential for timber use: Queensland maple, black cypress-pine, rosegum eucalyptus, and Amammanit eucalyptus. Several other species have good reforestation potential...

Diameter-density relationships provide tentative spacing guidelines for Eucalyptus saligna in Hawaii

Treesearch

Dean S. DeBell; Craig D. Whitesell

1988-01-01

Trials have been established in Hawaii to develop spacing guidelines for Eucalyptus saligna plantations. Substantial competition-related mortality occurred in densely planted plots of three spacing trials. Data on stand diameter and surviving number of trees on these plots were plotted in logarithmic form to estimate a "self-thinning" or...

Reinforcing Natural Rubber with Cellulose Nanofibrils Extracted from Bleached Eucalyptus Kraft Pulp

Treesearch

Chunmei Zhang; Tianliang Zhai; Ronald Sabo; Craig Clemons; Yi Dan; Lih-Sheng Turng

2014-01-01

Reinforced natural rubber (NR) nanocomposites were prepared by solution mixing, casting, and evaporation of pre-vulcanized natural rubber latex and an aqueous suspension of cellulose nanofibrils (CNFs) extracted from bleached eucalyptus kraft pulp. Scanning electron microscopy (SEM) images showed that there were no micro-scaled aggregates observed in the nanocomposites...

Forest products harvested in Hawaii-1969

Treesearch

Robert E. Burgan; Jr. Wesley H.C. Wong

1971-01-01

Primary forest products harvested in Hawaii in 1969 were valued at $331,000-a $3,000 drop from the value of the harvest surveyed in 1967. Sawlogs and veneer logs were the most important products. Koa and robusta eucalyptus were the primary sawlog species. Albizia and robusta eucalyptus provided most of the veneer logs.

Proceedings of a workshop on Eucalyptus in California; June 14-16, 1983; Sacramento, California

Treesearch

Richard B Standiford; F Thomas Ledig

1983-01-01

To provide up-to-date information on Eucalyptus in California, researchers from Califor-nia, Florida, Hawaii, Oregon, and France presented papers on species selection, products, uses, and economics, growth and yield, cultural requirements, propagation, and breeding programs. This Proceedings of the Workshop should serve as a useful reference for...

Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil

PubMed Central

Alfenas, R.F.; Lombard, L.; Pereira, O.L.; Alfenas, A.C.; Crous, P.W.

2015-01-01

Species in the genus Calonectria (Hypocreales) represent an important group of plant pathogenic fungi that cause serious losses to plant crops in tropical and subtropical climates. Calonectria leaf blight is currently one of the main impediments to Eucalyptus cultivation in Brazil, and various species of Calonectria have been associated with this disease. Since most previous identifications were solely based on morphological characters, much of the published literature needs to be re-evaluated. The aim of this study was thus to identify and determine the phylogenetic relationships among species that occur in the Eucalyptus growing regions of Brazil by using partial sequences of the β-tubulin, calmodulin, translation elongation factor 1-α and histone H3 gene regions. Based on extensive collections from soil and infected eucalypt leaf samples from plantations, phylogenetic inference revealed the Ca. pteridis complex to be the most common species complex present in Eucalyptus plantations in Brazil. By elucidating taxa in the Ca. pteridis, Ca. cylindrospora and Ca. candelabra species complexes, 20 novel Calonectria species were identified, and a new name in Calonectria provided for Cylindrocladium macrosporum as Ca. pseudopteridis. PMID:26955192

Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil.

PubMed

Alfenas, R F; Lombard, L; Pereira, O L; Alfenas, A C; Crous, P W

2015-03-01

Species in the genus Calonectria (Hypocreales) represent an important group of plant pathogenic fungi that cause serious losses to plant crops in tropical and subtropical climates. Calonectria leaf blight is currently one of the main impediments to Eucalyptus cultivation in Brazil, and various species of Calonectria have been associated with this disease. Since most previous identifications were solely based on morphological characters, much of the published literature needs to be re-evaluated. The aim of this study was thus to identify and determine the phylogenetic relationships among species that occur in the Eucalyptus growing regions of Brazil by using partial sequences of the β-tubulin, calmodulin, translation elongation factor 1-α and histone H3 gene regions. Based on extensive collections from soil and infected eucalypt leaf samples from plantations, phylogenetic inference revealed the Ca. pteridis complex to be the most common species complex present in Eucalyptus plantations in Brazil. By elucidating taxa in the Ca. pteridis, Ca. cylindrospora and Ca. candelabra species complexes, 20 novel Calonectria species were identified, and a new name in Calonectria provided for Cylindrocladium macrosporum as Ca. pseudopteridis.

Relation of the activities of the IPDF/INPE project (reforestation subproject) during the year 1979. [Mato Grosso do Sul, Brazil

NASA Technical Reports Server (NTRS)

Dejesusparada, N. (Principal Investigator); Filho, P. H.; Shimabukuro, Y. E.; Demedeiros, J. S.; Desantana, C. C.; Alves, E. C. M.

1981-01-01

The state of Mato Grosso do Sul was selected as the study area to define the recognizable classes of Eucalyptus spp. and Pinus spp. by visual and automatic analyses. For visual analysis, a preliminary interpretation key and a legend of 6 groups were derived. Based on these six groups, three final classes were defined for analysis: (1) area prepared for reforestation; (2) area reforested with Eucalyptus spp.; and (3) area reforested with Pinus spp. For automatic interpretation the area along the highway from Ribas do Rio Pardo to Agua Clara was classified into the following classes: eucalytus, bare soil, plowed soil, pine and "cerrado". The results of visual analysis show that 67% of the reforested farms have relative differences in area estimate below 5%, 22%, between 5% and 10%; and 11% between 10% and 20%. The reforested eucalyptus area is 17 times greater than the area of reforested pine. Automatic classification of eucalyptus ranged from 73.03% to 92.30% in the training areas.

Automatic classification of reforested Pinus SPP and Eucalyptus SPP in Mogi-Guacu, SP, Brazil, using LANDSAT data

NASA Technical Reports Server (NTRS)

Dejesusparada, N. (Principal Investigator); Shimabukuro, Y. E.; Hernandez, P. E.; Koffler, N. F.; Chen, S. C.

1978-01-01

The author has identified the following significant results. Single date LANDSAT CCTs were processed, by Image-100 to classify Pinus and Eucalyptus species and their age groups. The study area Mogi-Guagu was located in the humid subtropical climate zone of Sao Paulo. The study was divided into ten preliminary classes and featured selection algorithms were used to calculate Bhattacharyya distance between all possible pairs of these classes in the four available channels. Classes having B-distance values less than 1.30 were grouped in four classes: (1) class PE - P. elliottii, (2) class P0 - Pinus species other than P. elliotii, (3) class EY - Eucalyptus spp. under two years, and (4) class E0 - Eucalyptus spp. more than two years old. The percentages of correct classification ranged from 70.9% to 94.12%. Comparisons of acreage estimated from the Image-100 with ground truth data showed agreement. The Image-100 percent recognition values for the above four classes were 91.62%, 87.80%, 89.89%, and 103.30%, respectively.

Can the Understory Affect the Hymenoptera Parasitoids in a Eucalyptus Plantation?

PubMed Central

Dall’Oglio, Onice Teresinha; Ribeiro, Rafael Coelho; Ramalho, Francisco de Souza; Fernandes, Flávio Lemes; Wilcken, Carlos Frederico; de Assis Júnior, Sebastião Lourenço; Rueda, Rosa Angélica Plata; Serrão, José Eduardo; Zanuncio, José Cola

2016-01-01

The understory in forest plantations can increase richness and diversity of natural enemies due to greater plant species richness. The objective of this study was to test the hypothesis that the presence of the understory and climatic season in the region (wet or dry) can increase the richness and abundance of Hymenoptera parasitoids in Eucalyptus plantations, in the municipality of Belo Oriente, Minas Gerais State, Brazil. In each eucalyptus cultivation (five areas of cultivation) ten Malaise traps were installed, five with the understory and five without it. A total of 9,639 individuals from 30 families of the Hymenoptera parasitoids were collected, with Mymaridae, Scelionidae, Encyrtidae and Braconidae being the most collected ones with 4,934, 1,212, 619 and 612 individuals, respectively. The eucalyptus stands with and without the understory showed percentage of individuals 45.65% and 54.35% collected, respectively. The understory did not represent a positive effect on the overall abundance of the individuals Hymenoptera in the E. grandis stands, but rather exerted a positive effect on the specific families of the parasitoids of this order. PMID:26954578

Performance, kinetics, and equilibrium of methylene blue adsorption on biochar derived from eucalyptus saw dust modified with citric, tartaric, and acetic acids.

PubMed

Sun, Lei; Chen, Dongmei; Wan, Shungang; Yu, Zebin

2015-12-01

Biochar derived from eucalyptus saw dust modified with citric, tartaric, and acetic acids at low temperatures was utilized as adsorbent to remove methylene blue (MB) from aqueous solutions. Fourier transform infrared spectroscopy analysis showed that the carboxyl group was introduced on the biochar surface. Adsorption experiment data indicated that eucalyptus saw dust modified with citric acid showed higher MB adsorption efficiency than that modified with tartaric and acetic acids. Pseudo-second-order kinetics was the most suitable model for describing MB adsorption on biochar compared with pseudo-first-order, Elovich, and intraparticle diffusion models. The calculated values of ΔG(0) and ΔH(0) indicated the spontaneous and endothermic nature of the adsorption process. MB adsorption on biochar followed the Langmuir isotherm. The maximum adsorption capacities for eucalyptus saw dust modified with citric, tartaric, and acetic acids were 178.57, 99.01, and 29.94 mg g(-1), respectively, at 35°C. Copyright © 2015 Elsevier Ltd. All rights reserved.

Oligo-carrageenan kappa-induced reducing redox status and increase in TRR/TRX activities promote activation and reprogramming of terpenoid metabolism in Eucalyptus trees.

PubMed

González, Alberto; Gutiérrez-Cutiño, Marlen; Moenne, Alejandra

2014-06-05

In order to analyze whether the reducing redox status and activation of thioredoxin reductase (TRR)/thioredoxin(TRX) system induced by oligo-carrageenan (OC) kappa in Eucalyptus globulus activate secondary metabolism increasing terpenoid synthesis, trees were sprayed on the leaves with water, with OC kappa, or with inhibitors of NAD(P)H, ascorbate (ASC) and (GSH) synthesis and TRR activity, CHS-828, lycorine, buthionine sulfoximine (BSO) and auranofine, respectively, and with OC kappa and cultivated for four months. The main terpenoids in control Eucalyptus trees were eucalyptol (76%), α-pinene (7.4%), aromadendrene (3.6%), silvestrene (2.8%), sabinene (2%) and α-terpineol (0.9%). Treated trees showed a 22% increase in total essential oils as well as a decrease in eucalyptol (65%) and sabinene (0.8%) and an increase in aromadendrene (5%), silvestrene (7.8%) and other ten terpenoids. In addition, treated Eucalyptus showed seven de novo synthesized terpenoids corresponding to carene, α-terpinene, α-fenchene, γ-maaliene, spathulenol and α-camphenolic aldehyde. Most increased and de novo synthesized terpenoids have potential insecticidal and antimicrobial activities. Trees treated with CHS-828, lycorine, BSO and auranofine and with OC kappa showed an inhibition of increased and de novo synthesized terpenoids. Thus, OC kappa-induced reducing redox status and activation of TRR/TRX system enhance secondary metabolism increasing the synthesis of terpenoids and reprogramming of terpenoid metabolism in Eucalyptus trees.

Assessment of the floral origin of honey by SDS-page immunoblot techniques.

PubMed

Baroni, María V; Chiabrando, Gustavo A; Costa, Cristina; Wunderlin, Daniel A

2002-03-13

We report on the development of a novel alternative method for the assessment of floral origin in honey samples based on the study of honey proteins using immunoblot assays. The main goal of our work was to evaluate the use of honey proteins as chemical markers of the floral origin of honey. Considering that honeybee proteins should be common to all types of honey, we decided to verify the usefulness of pollen proteins as floral origin markers in honey. We used polyclonal anti-pollen antibodies raised in rabbits by repeated immunization of Sunflower (Elianthus annuus) and Eucalyptus (Eucalyptus sp.) pollen extracts. The IgG fraction was purified by immunoaffinity. These antibodies were verified with nitrocellulose blotted pollen and unifloral honey protein extracts. The antibodies anti-Sunflower pollen, bound to the 36 and 33 kDa proteins of Sunflower unifloral honey and to honey containing Sunflower pollen; and the antibodies anti-Eucalyptus sp. pollen bound to the 38 kDa proteins of Eucalyptus sp. unifloral honey in immunoblot assays. Satisfactory results were obtained in differentiating between the types of pollen analyzed and between Sunflower honey and Eucalyptus honey with less cross reactivity with other types of honey from different origin and also with good sensitivity in the detection. This immunoblot method opens an interesting field for the development of new antibodies from different plants, which could serve as an alternative or complementary method to the usual melissopalynological analysis to assess honey floral origin.

The Transcriptome and Terpene Profile of Eucalyptus grandis Reveals Mechanisms of Defense Against the Insect Pest, Leptocybe invasa.

PubMed

Oates, Caryn N; Külheim, Carsten; Myburg, Alexander A; Slippers, Bernard; Naidoo, Sanushka

2015-07-01

Plants have evolved complex defenses that allow them to protect themselves against pests and pathogens. However, there is relatively little information regarding the Eucalyptus defensome. Leptocybe invasa is one of the most damaging pests in global Eucalyptus forestry, and essentially nothing is known regarding the molecular mechanisms governing the interaction between the pest and host. The aim of the study was to investigate changes in the transcriptional landscape and terpene profile of a resistant and susceptible Eucalyptus genotype in an effort to improve our understanding of this interaction. We used RNA-seqencing to investigate transcriptional changes following L. invasa oviposition. Expression levels were validated using real-time quantitative PCR. Terpene profiles were investigated using gas chromatography coupled to mass spectometry on uninfested and oviposited leaves. We found 698 and 1,115 significantly differentially expressed genes from the resistant and susceptible interactions, respectively. Gene Ontology enrichment and Mapman analyses identified putative defense mechanisms including cell wall reinforcement, protease inhibitors, cell cycle suppression and regulatory hormone signaling pathways. There were significant differences in the mono- and sesquiterpene profiles between genotypes and between control and infested material. A model of the interaction between Eucalyptus and L. invasa was proposed from the transcriptomic and chemical data. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract

PubMed Central

Reigosa, Manuel J.; Valentão, Patrícia; Andrade, Paula B.

2018-01-01

In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside) and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids) were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus aqueous extract would be discarded in post-emergence, whereas it was promising as a pre-emergence bioherbicide. PMID:29438430

ANTI-INFLAMMATORY ACTIVITY OF EUCALYPTUS SPP. AND PISTASCIA LENTISCUS LEAF EXTRACTS.

PubMed

Qabaha, Khaled; Ras, Sari Abu; Abbadi, Jehad; Al-Rimawi, Fuad

2016-01-01

Eucalyptus spp. and Pistascia lentiscus are among the Palestinian trees that are traditionally used in folkloric medicine in treating many diseases; leaves of which are thought to have anti-inflammatory, antibacterial and antioxidant effects. The goal of this study is to evaluate the in vitro inhibitory effect of Eucalyptus spp . and Pistascia lentiscus extracts on Lipopolysacaride (LPS)-induced Interlukin-6 (Il-6) and Tumor Necrosis Factor-α (TNF-α) by polymorphonuclear Cells (PMNCs). Polymorphonuclear cells were isolated from the whole blood using Histopaque (Ficol-1077) method and then cultured in an enriched Roswell Park Memorial Institute (RBMI) medium. Supernatants' Interlukin-6 (IL-6) and Tumor Necrosis Factor (TNF-α) levels were determined 24 hour after LPS stimulation. HPLC was employed to determine the concentration of phenolic compounds in the extracts. The concentrations of TNF-α and IL-6 were compared using paired-samples t test. Eucalyptus spp . and Pistascia lentiscus leaves extracts have shown significant reduction in the levels of both Il-6 and TNF-α Gallic acid; a strong anti-inflammatory agent was found to be the major phenolic compound in both leaf extracts. However, other anti-inflammatory phenolic compounds were detected in Pitascia lentiscus extract including syringic acid and p-coumaric acid, while chlorogenic acid was detected in Eucalyptus spp . leaf extract. Reduction in the levels of Il-6 and TNF-α upon the effect of both Eucalyptus spp . and Pistascia lentiscus extract is an indication of their anti-inflammatory effects. Our results may also indicate that the observed anti-inflammatory effect of the above extracts may be due to the presence of gallic acid and other phenolic compounds. List of Abbreviations and Nomenclature: LPS: Lipopolysacaride, Il-6: Interlukin-6, TNF-α: Tumor Necrosis Factor-α, PMNCs: Polymorphonuclear Cells, HPLC: High Performance Liquid Chromatography, ELISA: Enzyme Linked Immune Sorbent Assay, EDTA: Ethylene Diamine Tetra Acetic acid, PBS: phosphate buffered saline, RPMI: Roswell Park Memorial Institute medium FBS: Fetal Bovine Serum.

ANTI-INFLAMMATORY ACTIVITY OF EUCALYPTUS SPP. AND PISTASCIA LENTISCUS LEAF EXTRACTS

PubMed Central

Qabaha, Khaled; Ras, Sari Abu; Abbadi, Jehad; Al-Rimawi, Fuad

2016-01-01

Background: Eucalyptus spp. and Pistascia lentiscus are among the Palestinian trees that are traditionally used in folkloric medicine in treating many diseases; leaves of which are thought to have anti-inflammatory, antibacterial and antioxidant effects. The goal of this study is to evaluate the in vitro inhibitory effect of Eucalyptus spp. and Pistascia lentiscus extracts on Lipopolysacaride (LPS)-induced Interlukin-6 (Il-6) and Tumor Necrosis Factor-α (TNF-α) by polymorphonuclear Cells (PMNCs). Materials and Methods: Polymorphonuclear cells were isolated from the whole blood using Histopaque (Ficol-1077) method and then cultured in an enriched Roswell Park Memorial Institute (RBMI) medium. Supernatants’ Interlukin-6 (IL-6) and Tumor Necrosis Factor (TNF-α) levels were determined 24 hour after LPS stimulation. HPLC was employed to determine the concentration of phenolic compounds in the extracts. The concentrations of TNF-α and IL-6 were compared using paired-samples t test. Results: Eucalyptus spp. and Pistascia lentiscus leaves extracts have shown significant reduction in the levels of both Il-6 and TNF-α Gallic acid; a strong anti-inflammatory agent was found to be the major phenolic compound in both leaf extracts. However, other anti-inflammatory phenolic compounds were detected in Pitascia lentiscus extract including syringic acid and p-coumaric acid, while chlorogenic acid was detected in Eucalyptus spp. leaf extract. Conclusion: Reduction in the levels of Il-6 and TNF-α upon the effect of both Eucalyptus spp. and Pistascia lentiscus extract is an indication of their anti-inflammatory effects. Our results may also indicate that the observed anti-inflammatory effect of the above extracts may be due to the presence of gallic acid and other phenolic compounds. List of Abbreviations and Nomenclature: LPS: Lipopolysacaride, Il-6: Interlukin-6, TNF-α: Tumor Necrosis Factor-α, PMNCs: Polymorphonuclear Cells, HPLC: High Performance Liquid Chromatography, ELISA: Enzyme Linked Immune Sorbent Assay, EDTA: Ethylene Diamine Tetra Acetic acid, PBS: phosphate buffered saline, RPMI: Roswell Park Memorial Institute medium FBS: Fetal Bovine Serum. PMID:28487887

Special trends in CBF and DREB2 groups in Eucalyptus gunnii vs Eucalyptus grandis suggest that CBF are master players in the trade-off between growth and stress resistance.

PubMed

Nguyen, Hong C; Cao, Phi B; San Clemente, Hélène; Ployet, Raphaël; Mounet, Fabien; Ladouce, Nathalie; Harvengt, Luc; Marque, Christiane; Teulieres, Chantal

2017-04-01

Annotation of the Eucalyptus grandis genome showed a large amplification of the dehydration-responsive element binding 1/C-repeat binding factor (DREB1/CBF) group without recent DREB2 gene duplication compared with other plant species. The present annotation of the CBF and DREB2 genes from a draft of the Eucalyptus gunnii genome sequence reveals at least one additional CBF copy in the E. gunnii genome compared with E. grandis, suggesting that this group is still evolving, unlike the DREB2 group. This study aims to investigate the redundancy/neo- or sub-functionalization of the duplicates and the relative involvement of the two groups in abiotic stress responses in both E. grandis and E. gunnii (lower growth but higher cold resistance). A comprehensive transcriptional analysis using high-throughput quantitative real-time polymerase chain reaction (qRT-PCR) was performed on leaves, stems and roots from the two Eucalyptus species after cold, heat or drought treatment. A large CBF cluster accounted for most of the cold response in all the organs, whereas heat and drought responses mainly involved a small CBF cluster and the DREB2 genes. In addition, CBF putative target genes, known to be involved in plant tolerance and development, were found to be cold-regulated. The higher transcript amounts of both the CBF and target genes in the cold tolerant E. gunnii contrasted with the higher CBF induction rates in the fast growing E. grandis. Altogether, the present results, in agreement with previous data about Eucalyptus transgenic lines over-expressing CBF, suggest that these factors, which promote both stress protection and growth limitation, participate in the trade-off between growth and resistance in this woody species. © 2016 Scandinavian Plant Physiology Society.

Unravelling the bioherbicide potential of Eucalyptus globulus Labill: Biochemistry and effects of its aqueous extract.

PubMed

Puig, Carolina G; Reigosa, Manuel J; Valentão, Patrícia; Andrade, Paula B; Pedrol, Nuria

2018-01-01

In the worldwide search for new strategies in sustainable weed management, the use of plant species able to produce and release phytotoxic compounds into the environment could be an effective alternative to synthetic herbicides. Eucalyptus globulus Labill. is known to be a source of biologically active compounds responsible for its phytotoxic and allelopathic properties. Our previous results demonstrated the bioherbicide potential of eucalyptus leaves incorporated into the soil as a green manure, probably through the release of phytotoxins into the soil solution. Thus, the aims of this study were to understand the phytotoxicity of the eucalyptus leaves aqueous extract applied in pre- and post-emergence, and to identify and quantify its potentially phytotoxic water-soluble compounds. The effects were tested on the germination and early growth of the model target species Lactuca sativa and Agrostis stolonifera, and on physiological parameters of L. sativa adult plants after watering or spraying application. Dose-response curves and ED50 and ED80 values for eucalyptus aqueous extracts revealed pre-emergence inhibitory effects on both target species, effects being comparable to the herbicide metolachlor. While spraying treatment reduced the aerial and root biomass and increased the dry weight/fresh weight ratio of lettuce adult plants, watering application reduced protein contents and chlorophyll concentrations with respect to control, reflecting different modes of action depending on the site of phytotoxin entry. Via HPLC analyses, a total of 8 phenolic compounds (chlorogenic, two ρ-coumaric derivatives, ellagic, hyperoside, rutin, quercitrin, and kaempferol 3-O-glucoside) and other 5 low weight organic acids (citric, malic, shikimic, succinic and fumaric acids) were obtained from aqueous extract, the latter being identified for the first time in E. globulus. Despite some phytotoxic effects were found on lettuce adult plants, the use of eucalyptus aqueous extract would be discarded in post-emergence, whereas it was promising as a pre-emergence bioherbicide.

Chemical composition of 8 eucalyptus species' essential oils and the evaluation of their antibacterial, antifungal and antiviral activities

PubMed Central

2012-01-01

Background In 1957, Tunisia introduced 117 species of Eucalyptus; they have been used as fire wood, for the production of mine wood and to fight erosion. Actually, Eucalyptus essential oil is traditionally used to treat respiratory tract disorders such as pharyngitis, bronchitis, and sinusitis. A few investigations were reported on the biological activities of Eucalyptus oils worldwide. In Tunisia, our previous works conducted in 2010 and 2011 had been the first reports to study the antibacterial activities against reference strains. At that time it was not possible to evaluate their antimicrobial activities against clinical bacterial strains and other pathogens such as virus and fungi. Methods The essential oils of eight Eucalyptus species harvested from the Jbel Abderrahman, Korbous (North East Tunisia) and Souinet arboreta (North of Tunisia) were evaluated for their antimicrobial activities by disc diffusion and microbroth dilution methods against seven bacterial isolates: Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pneumoniae and Streptococcus pyogenes. In addition, the bactericidal, fungicidal and the antiviral activities of the tested oils were carried out. Results Twenty five components were identified by GC/FID and GC/MS. These components were used to correlate with the biological activities of the tested oils. The chemical principal component analysis identified three groups, each of them constituted a chemotype. According to the values of zone diameter and percentage of the inhibition (zdi, % I, respectively), four groups and subgroups of bacterial strains and three groups of fungal strains were characterized by their sensitivity levels to Eucalyptus oils. The cytotoxic effect and the antiviral activity varied significantly within Eucalyptus species oils. Conclusions E. odorata showed the strongest activity against S. aureus, H. influenzae, S. agalactiae, S. pyogenes, S. pneumoniae and against all the tested fungal strains. In addition, E. odorata oil showed the most cytotoxic effect. However, the best antiviral activity appeared with E. bicostata. Virus pretreatment with E. bicostata essential oil showed better antiviral activity (IC50 = 0.7 mg/ml, SI = 22.8) than cell-pretreatment (IC50 = 4.8 mg/ml, SI = 3.33). The essential oil of E. astringens showed antiviral activity only when incubated with virus prior to cell infection. This activity was dose-dependent and the antiviral activity diminished with the decreasing essential oil concentration. PMID:22742534

Survey to evaluate escape of Eucalyptus spp. seedlings from plantations in southeastern USA

Treesearch

Mac A. Callaham; John A. Stanturf; William J. Hammond; Donald L. Rockwood; Evelyn S. Wenk; Joseph J. O’Brien

2013-01-01

Interest in biomass-based energy in the southeastern Unites States has led to increased need for fast-growing tree species. Several Eucalyptus species exhibit characteristics that make them attractive in the bioenergy context. However, some of these also possess traits that suggest they could become invasive. To make a preliminary assessment of the...

Cut-to-length harvesting of short-rotation Eucalyptus

Treesearch

Bruce R. Hartsough; David J. Cooper

1999-01-01

Traditional whole-tree harvesting systems work well in short-rotation hardwood plantations, but other methods are needed where it is desirable to leave the residues on the site. We tested a system consisting of a cut-to-length harvester, forwarder, mobile chipper, and chip screen to clearcut a 7-year-old plantation of Eucalyptus viminalis. Three...

Benefits of Eucalyptus-Albizia mixtures vary by site on Hawaii Island

Treesearch

Dean S. DeBell; Craig D. Whitesell; Thomas B. Crabb

1987-01-01

Eucalyptus saligna Sm. plantations are being tested in Hawaii as a means of providing biomass for conversion to energy. Initial growth rates have been excellent, but supplemental nitrogen (N) is needed for sustained productivity on most sites. Although responses to N fertilizer have been substantial, costs of N applications are costly in dollars and...

Utilization of eucalyptus for bioelectricity production in brazil via fast pyrolysis: a techno-economic analysis

USDA-ARS?s Scientific Manuscript database

In this study, a process model of a 2000 metric ton per day (MTPD) eucalyptus Tail Gas Reactive Pyrolysis (TGRP) and electricity generation plant was developed and simulated in SimSci Pro/II software for the purpose of evaluating its techno-economic viability in Brazil. Two scenarios were compared b...

Comparing second year growth of American sycamore, black willow, and eastern cottonwood with and without fertilization

Treesearch

Jamie L. Schuler

2012-01-01

Interest in developing domestically produced bio-based fuel systems has been responsible for a large increase in short rotation woody crop (SRWC) research. Much of this work has been used in developing regional production estimates for woody crops like cottonwood (Populus deltoides Bartr. ex Marsh.) and eucalyptus (Eucalyptus spp...

Soil conservation service tests of Eucalyptus species for windbreaks

Treesearch

Gary L. Young

1983-01-01

The Soil Conservation Service is in the early stages of testing many species of Eucalyptus or windbreaks. Over 260 different species have been collected. The pre-planting selection criteria and process is described as well as the test conditions and procedures. Some sources of information on the use of the Eucalypts may be misleading through...

Lumber potential of 12-year-old Saligna eucalyptus trees in Hawaii

Treesearch

Roger G. Skolmen

1974-01-01

The sawtimber potential of 12-year-old Eucalyptus saligna Sm. trees grown in Maui, Hawaii, was studied by using simple field techniques. Lumber manufactured from the trees was predominantly of low grade, but had lower average density than wood from older trees, and therefore was more desirable. Logs end-split badly, and sawed with the usually...

Managing for water-use efficient wood production in Eucalyptus globulus plantations

Treesearch

Donald A. White; John F. McGrath; Michael G. Ryan; Michael Battaglia; Daniel S. Mendham; Joe Kinal; Geoffrey M. Downes; D. Stuart Crombie; Mark E. Hunt

2014-01-01

This paper tests the hypothesis that thinning and nitrogen fertiliser can increase the mass of wood produced per volume of water used (evapotranspiration) by plantations of Eucalyptus globulus. We have called this plantation water productivity (PWPWOOD) and argue that, for a given genotype, this term integrates the effects of management, site and climate on both...

Energy product options for Eucalyptus species grown as short rotation woody crops

Treesearch

Donald Rockwood; Alan W. Rudie; Sally A. Ralph; J.Y. Zhu; Jerrold E. Winandy

2008-01-01

Eucalyptus species are native to Australia but grown extensively worldwide as short rotation hardwoods for a variety of products and as ornamentals. We describe their general importance with specific emphasis on existing and emerging markets as energy products and the potential to maximize their productivity as short rotation woody crops. Using experience in Florida...

Establishing a Eucalyptus energy plantation on the central coast of California

Treesearch

Norman H. Pillsbury; Nelson L. Ayers

1983-01-01

A 17.5-acre non-irrigated biomass energy plantation has been established near San Luis Obispo. This joint California Polytechnic State University - California Department of Forestry project is measuring plot growth response of seven eucalyptus species for three spacing trials and for the effect of fertilization. All study plots are replicated. Site preparation strategy...

Short-rotation management of Eucalyptus: guidelines for plantations in Hawaii

Treesearch

Craig D. Whitesell; Dean S. DeBell; Thomas H. Schubert; Robert F. Strand; Thomas B. Crabb

1992-01-01

A 10-year research and development program was conducted by the BioEnergy Development Corporation, USDA Forest Service, and U.S. Department of Energy on the island of Hawaii, where nearly 230,000 acres are suitable for growing biomass in short-rotation Eucalyptus plantations. Successful techniques are described for seedling production, plantation establishment (site...

Properties of cellulose micro/nanofibers obtained from eucalyptus pulp fiber treated with anaerobic digestate and high shear mixing

USDA-ARS?s Scientific Manuscript database

High production costs remain the single greatest factor limiting wider use of cellulose micro/nanofibers in the industry. The objective of the present study was to investigate the potential of using a low-cost bacteria-rich digestate (liquid anaerobic digestate – AD supernatant) on milled eucalyptus...

Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils.

PubMed

Delaquis, Pascal J; Stanich, Kareen; Girard, Benoit; Mazza, G

2002-03-25

Essential oils from dill (Anethum graveolens L.), coriander (seeds of Coriandrum sativum L.), cilantro (leaves of immature C. sativum L.) and eucalyptus (Eucalyptus dives) were separated into heterogeneous mixtures of components by fractional distillation and were analyzed by gas chromatography-mass spectroscopy. Minimum inhibitory concentrations against gram-positive bacteria, gram-negative bacteria and Saccharomyces cerevisiae were determined for the crude oils and their fractions. Essential oil of cilantro was particularly effective against Listeria monocytogenes, likely due to the presence of long chain (C6-C10) alcohols and aldehydes. The strength and spectrum of inhibition for the fractions often exceeded those determined in the crude oils. Mixing of fractions resulted in additive, synergistic or antagonistic effects against individual test microorganisms.

Essential oil composition of Eucalyptus microtheca and Eucalyptus viminalis.

PubMed

Maghsoodlou, Malek Taher; Kazemipoor, Nasrin; Valizadeh, Jafar; Falak Nezhad Seifi, Mohsen; Rahneshan, Nahid

2015-01-01

Eucalyptus (Fam. Myrtaceae) is a medicinal plant and various Eucalyptus species possess potent pharmacological actions against diabetes, hepatotoxicity, and inflammation. This study aims to investigate essential oil composition from leaves and flowers of E. microtheca and E. viminalis leaves growing in the Southeast of Iran. The aerial parts of these plants were collected from Zahedan, Sistan and Baluchestan province, Iran in 2013. After drying the plant materials in the shade, the chemical composition of the essential oils was obtained by hydro-distillation method using a Clevenger-type apparatus and analyzed by GC/MS. In the essential oil of E. microtheca leaves, 101 compounds representing 100%, were identified. Among them, α-phellandrene (16.487%), aromadendrene (12.773%), α-pinene (6.752%), globulol (5.997%), ledene (5.665%), P-cymen (5.251%), and β-pinene (5.006%) were the major constituents. In the oil of E. microtheca flowers, 88 compounds representing 100%, were identified in which α-pinene (16.246%), O-cymen (13.522%), β-pinene (11.082%), aromadendrene (7.444%), α-phellandrene (7.006%), globulol (5.419%), and 9-octadecenamide (5.414%) were the major components. Sixty six compounds representing 100% were identified in the oil of E. viminalis leaves. The major compounds were 1, 8-cineole (57.757%), α-pinene (13.379%), limonene (5.443%), and globulol (3.054%). The results showed the essential oils from the aerial parts of Eucalyptus species are a cheap source for the commercial isolation of α-phellandrene, α-pinene, and 1, 8-cineole compounds to be used in medicinal and food products. Furthermore, these plants could be an alternative source of insecticide agents.

Ultra-high performance liquid chromatography coupled to mass spectrometry applied to the identification of valuable phenolic compounds from Eucalyptus wood.

PubMed

Santos, Sónia A O; Vilela, Carla; Freire, Carmen S R; Neto, Carlos Pascoal; Silvestre, Armando J D

2013-11-01

Ultra-high performance liquid chromatography (UHPLC) was applied for the first time in the analysis of wood extracts. The potential of this technique coupled to ion trap mass spectrometry in the rapid and effective detection and identification of bioactive components in complex vegetal samples was demonstrated. Several dozens of compounds were detected in less than 30min of analysis time, corresponding to more than 3-fold reduction in time, when compared to conventional HPLC analysis of similar extracts. The phenolic chemical composition of Eucalyptus grandis, Eucalyptus urograndis (E. grandis×E. urophylla) and Eucalyptus maidenii wood extracts was assessed for the first time, with the identification of 51 phenolic compounds in the three wood extracts. Twenty of these compounds are reported for the first time as Eucalyptus genus components. Ellagic acid and ellagic acid-pentoside are the major components in all extracts, followed by gallic and quinic acids in E. grandis and E. urograndis and ellagic acid-pentoside isomer, isorhamnetin-hexoside and gallic acid in E. maidenii. The antioxidant scavenging activity of the extracts was evaluated, with E. grandis wood extract showing the lowest IC50 value. Moreover, the antioxidant activity of these extracts was higher than that of the commercial antioxidant BHT and of those of the corresponding bark extracts. These results, together with the phenolic content values, open good perspectives for the exploitation of these renewable resources as a source of valuable phenolic compounds. Copyright © 2013 Elsevier B.V. All rights reserved.

Comprehensive genetic dissection of wood properties in a widely-grown tropical tree: Eucalyptus

PubMed Central

2011-01-01

Background Eucalyptus is an important genus in industrial plantations throughout the world and is grown for use as timber, pulp, paper and charcoal. Several breeding programmes have been launched worldwide to concomitantly improve growth performance and wood properties (WPs). In this study, an interspecific cross between Eucalyptus urophylla and E. grandis was used to identify major genomic regions (Quantitative Trait Loci, QTL) controlling the variability of WPs. Results Linkage maps were generated for both parent species. A total of 117 QTLs were detected for a series of wood and end-use related traits, including chemical, technological, physical, mechanical and anatomical properties. The QTLs were mainly clustered into five linkage groups. In terms of distribution of QTL effects, our result agrees with the typical L-shape reported in most QTL studies, i.e. most WP QTLs had limited effects and only a few (13) had major effects (phenotypic variance explained > 15%). The co-locations of QTLs for different WPs as well as QTLs and candidate genes are discussed in terms of phenotypic correlations between traits, and of the function of the candidate genes. The major wood property QTL harbours a gene encoding a Cinnamoyl CoA reductase (CCR), a structural enzyme of the monolignol-specific biosynthesis pathway. Conclusions Given the number of traits analysed, this study provides a comprehensive understanding of the genetic architecture of wood properties in this Eucalyptus full-sib pedigree. At the dawn of Eucalyptus genome sequence, it will provide a framework to identify the nature of genes underlying these important quantitative traits. PMID:21651758

Contrasting physiological responses of six eucalyptus species to water deficit.

PubMed

Merchant, Andrew; Callister, Andrew; Arndt, Stefan; Tausz, Michael; Adams, Mark

2007-12-01

The genus Eucalyptus occupies a broad ecological range, forming the dominant canopy in many Australian ecosystems. Many Eucalyptus species are renowned for tolerance to aridity, yet inter-specific variation in physiological traits, particularly water relations parameters, contributing to this tolerance is weakly characterized only in a limited taxonomic range. The study tests the hypothesis that differences in the distribution of Eucalyptus species is related to cellular water relations. Six eucalypt species originating from (1) contrasting environments for aridity and (2) diverse taxonomic groups were grown in pots and subjected to the effects of water deficit over a 10-week period. Water potential, relative water content and osmotic parameters were analysed by using pressure-volume curves and related to gas exchange, photosynthesis and biomass. The six eucalypt species differed in response to water deficit. Most significantly, species from high rainfall environments (E. obliqua, E. rubida) and the phreatophyte (E. camaldulensis) had lower osmotic potential under water deficit via accumulation of cellular osmotica (osmotic adjustment). In contrast, species from low rainfall environments (E. cladocalyx, E. polyanthemos and E. tricarpa) had lower osmotic potential through a combination of both constitutive solutes and osmotic adjustment, combined with reductions in leaf water content. It is demonstrated that osmotic adjustment is a common response to water deficit in six eucalypt species. In addition, significant inter-specific variation in osmotic potential correlates with species distribution in environments where water is scarce. This provides a physiological explanation for aridity tolerance and emphasizes the need to identify osmolytes that accumulate under stress in the genus Eucalyptus.

First insights into the functional role of vasicentric tracheids and parenchyma in eucalyptus species with solitary vessels: do they contribute to xylem efficiency or safety?

PubMed

Barotto, Antonio José; Fernandez, María Elena; Gyenge, Javier; Meyra, Ariel; Martinez-Meier, Alejandro; Monteoliva, Silvia

2016-12-01

The relationship between hydraulic specific conductivity (k s ) and vulnerability to cavitation (VC) with size and number of vessels has been studied in many angiosperms. However, few of the studies link other cell types (vasicentric tracheids (VT), fibre-tracheids, parenchyma) with these hydraulic functions. Eucalyptus is one of the most important genera in forestry worldwide. It exhibits a complex wood anatomy, with solitary vessels surrounded by VT and parenchyma, which could serve as a good model to investigate the functional role of the different cell types in xylem functioning. Wood anatomy (several traits of vessels, VT, fibres and parenchyma) in conjunction with maximum k s and VC was studied in adult trees of commercial species with medium-to-high wood density (Eucalyptus globulus Labill., Eucalyptus viminalis Labill. and Eucalyptus camaldulensis Dehnh.). Traits of cells accompanying vessels presented correlations with functional variables suggesting that they contribute to both increasing connectivity between adjacent vessels-and, therefore, to xylem conduction efficiency-and decreasing the probability of embolism propagation into the tissue, i.e., xylem safety. All three species presented moderate-to-high resistance to cavitation (mean P 50 values = -2.4 to -4.2 MPa) with no general trade-off between efficiency and safety at the interspecific level. The results in these species do not support some well-established hypotheses of the functional meaning of wood anatomy. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress

PubMed Central

Xie, Qingqing; Li, Zhenji; Yang, Limin; Lv, Jing; Jobe, Timothy O.; Wang, Qiuquan

2015-01-01

Manganese (Mn) is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM) X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry) lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification. PMID:26327118

Eucalyptus globulus extract protects upon acetaminophen-induced kidney damages in male rat

PubMed Central

Dhibi, Sabah; Mbarki, Sakhria; Elfeki, Abdelfettah; Hfaiedh, Najla

2014-01-01

Plants have historically been used in treating many diseases. Eucalyptus globules, a rich source of bioactive compounds, and have been shown to possess antioxidative properties. The purpose of this study, carried out on male Wistar rats, was to evaluate the beneficial effects of Eucalyptus globulus extract upon acetaminophen-induced damages in kidney. Our study is realized in the Department of Biology, Faculty of Sciences of Sfax (Tunisia). 32 Wistar male rats; were divided into 4 batches: a control group (n=8), a group of rats treated with acetaminophen (goomg/kg) by intraperitoneal injection during 4 days (n=8), a group receiving Eucalyptus globulus extract (130 mg of dry leaves/kg/day) in drinking water during 42 days after 2 hours of acetaminophen administration (during 4 days) (n=8) and group received only Eucalyptus (n=8) during 42 days. After 6 weeks, animals from each group were rapidly sacrificed by decapitation. Blood serum was obtained by centrifugation. Under our experimental conditions, acetaminophen poisoning resulted in an oxidative stress evidenced by statistically significant losses in the activities of catalase (CAT), superoxide-dismutase (SOD), glutathione-peroxidase (GPX) activities and an increase in lipids peroxidation level in renal tissue of acetaminophen-treated group compared with the control group. Acetaminophen also caused kidney damage as evident by statistically significant (p<0.05) increase in levels of creatinine and urea and decreased levels of uric acid and proteins in blood. Histological analysis demonstrated alteration of proximal tubules, atrophy of the glomerule and dilatation of urinary space. Previous administration of plant extract is found to alleviate this acetaminophen-induced damage. PMID:24856382

A Newly Identified Passive Hyperaccumulator Eucalyptus grandis × E. urophylla under Manganese Stress.

PubMed

Xie, Qingqing; Li, Zhenji; Yang, Limin; Lv, Jing; Jobe, Timothy O; Wang, Qiuquan

2015-01-01

Manganese (Mn) is an essential micronutrient needed for plant growth and development, but can be toxic to plants in excess amounts. However, some plant species have detoxification mechanisms that allow them to accumulate Mn to levels that are normally toxic, a phenomenon known as hyperaccumulation. These species are excellent candidates for developing a cost-effective remediation strategy for Mn-polluted soils. In this study, we identified a new passive Mn-hyperaccumulator Eucalyptus grandis × E. urophylla during a field survey in southern China in July 2010. This hybrid can accumulate as much as 13,549 mg/kg DW Mn in its leaves. Our results from Scanning Electron Microscope (SEM) X-ray microanalysis indicate that Mn is distributed in the entire leaf and stem cross-section, especially in photosynthetic palisade, spongy mesophyll tissue, and stem xylem vessels. Results from size-exclusion chromatography coupled with ICP-MS (Inductively coupled plasma mass spectrometry) lead us to speculate that Mn associates with relatively high molecular weight proteins and low molecular weight organic acids, including tartaric acid, to avoid Mn toxicity. Our results provide experimental evidence that both proteins and organic acids play important roles in Mn detoxification in Eucalyptus grandis × E. urophylla. The key characteristics of Eucalyptus grandis × E. urophylla are an increased Mn translocation facilitated by transpiration through the xylem to the leaves and further distribution throughout the leaf tissues. Moreover, the Mn-speciation profile obtained for the first time in different cellular organelles of Eucalyptus grandis × E. urophylla suggested that different organelles have differential accumulating abilities and unique mechanisms for Mn-detoxification.

Lumber grade recoverey from Hawaii-grown robusta Eucalyptus logs

Treesearch

Roger G. Skolmen

1970-01-01

In part to supplement meager data on lumber grade yield of Hawaii-grown timber, 30 robusta eucalyptus logs were shipped to a Michigan sawmill for processing. The logs were from 12 trees in three different stands. The lumber produced was graded according to National Hardwood Lumber Association standards. The sample was too small to provide a basis for predicting grade...

Cristulariella moricola associated with foliar blight of Camden white gum ( Eucalyptus benthamii ), a bioenergy crop

Treesearch

Doug P. Aubrey; Stephen W. Fraedrich; Thomas C. Harrington; Rabiu Olatinwo

2017-01-01

We report the association of Cristulariella moricola (Hino) Redhead with a zonate leaf spot on Eucalyptus benthamii (Myrtaceae). We observed the disease outbreak across a series of experimental E. benthamii plantings in Barnwell County, South Carolina, USA in October 2015. The disease caused nearly complete foliar necrosis of most trees in one experimental planting,...

Response of Eucalyptus species to frost damage at the Redwood Experimental Forest

Treesearch

Danny G. Heavilin

1978-01-01

In 1961 a feasibility study was done to determine if certain Eucalyptus species could be grown along the California North Coast for timber or ornamental purposes. Of the 207 trees, representing 31 species, planted on the Redwood Experimental Forest, only 12 trees in 5 species were recorded in the 1976 survival examination. The single most damaging factor was frost....

Direct seeding of lemon-gum eucalyptus, redwood, and brushbox in Hawaii

Treesearch

Gerald A. Waiters

1970-01-01

Direct seeding has economic and silvicultural advantages over planted seedlings. To see if three selected timber species could be direct-seeded, trials were held at Kulani Camp, island of Hawaii. After 1 year, lemon-gum eucalyptus had fair stocking and height growth, but redwood and brushbox had not progressed satisfactorily. Mulch had no real effect on either stocking...

The Brazil Eucalyptus Potential Productivity Project: Influence of water, nutrients and stand uniformity on wood production

Treesearch

Jose Luiz Stape; Dan Binkley; Michael G. Ryan; Sebastiao Fonseca; Rodolfo A. Loos; Ernesto N. Takahashi; Claudio R. Silva; Sergio R. Silva; Rodrigo E. Hakamada; Jose Mario de A. Ferreira; Augusto M. N. Lima; Jose Luiz Gava; Fernado P. Leite; Helder B. Andrade; Jacyr M. Alves; Gualter G. C. Silva; Moises R. Azevedo

2010-01-01

We examined the potential growth of clonal Eucalyptus plantations at eight locations across a 1000+ km gradient in Brazil by manipulating the supplies of nutrients and water, and altering the uniformity of tree sizes within plots. With no fertilization or irrigation, mean annual increments of stem wood were about 28% lower (16.2 Mg...

A principal component approach for predicting the stem volume in Eucalyptus plantations in Brazil using airborne LiDAR data

Treesearch

Carlos Alberto Silva; Carine Klauberg; Andrew T. Hudak; Lee A. Vierling; Veraldo Liesenberg; Samuel P. C. e Carvalho; Luiz C. E. Rodriguez

2016-01-01

Improving management practices in industrial forest plantations may increase production efficiencies, thereby reducing pressures on native tropical forests for meeting global pulp needs. This study aims to predict stem volume (V) in plantations of fast-growing Eucalyptus hybrid clones located in southeast Brazil using field plot and airborne Light Detection...

Breeding Eucalyptus for disease resistance

Treesearch

Edival A.V. Zauza; Acelino Couto Alfenas; Lúcio Mauro da Silva Guimarães; João Flávio da Silva

2012-01-01

Eucalyptus plantations cover about 1.5 percent of the agricultural area in Brazil, and contribute to 4 percent of GDP and 3 percent in exports of forest products. Technological and research advances in silviculture and genetic improvement have increased productivity up to 80 m³ of wood/ha/year, with an average of 35 to 45 m³/ha/year. The greatest...

Clonal propagation on Eucalyptus by cuttings in France

Treesearch

H. Chaperon

1983-01-01

A.FO.CEL has developed a technique for mass propagation by cuttings of Eucalyptus in France. This technique is described from the selection of the ortet to the mass propagation of the clone for afforestation: the first stage is the mobilization of the ortet, the second stage is called pre-propagation which includes rejuvenating and rooting conditioning, the third stage...

Yields in high density, short rotation intensive culture (SRIC)—plantations of Eucalyptus and Other Hardwood Species

Treesearch

R.M. Sachs; C.B. Low

1983-01-01

Initial high density (17,200 trees ha-1, 6961 trees a-1) plantations of Eucalyptus grandis yielded up to 22 oven dry tons (ODT) ha-l yr-I (10 ta-1 yr-1) on an approximate 6 month rotation. Border effects could not be eliminated from the small sized plots used...

Evaluating potential changes in fire risk from Eucalyptus plantings in the Southern United States

Treesearch

Scott L. Goodrick; John A. Stanturf

2012-01-01

Renewed interest in short-rotation woody crops for bioenergy and bioproducts has prompted a reevaluation of the Eucalyptus species for the southern United States. One question that arises about the potential effects of introducing a nonnative species is what effect will there be on fire behavior. Our approximate answer based on modeling fire behavior...

Fluidized bed catalytic pyrolysis of eucalyptus over hzsm-5: effect of acid density and gallium modification on catalyst deactivation

USDA-ARS?s Scientific Manuscript database

Catalytic fast pyrolysis of eucalyptus wood was performed on a continuous laboratory scale fluidized bed fast pyrolysis system. Catalytic activity was monitored from use of fresh catalyst up to a cumulative biomass to catalyst ratio (B/C) of 4/1 over extruded pellets of three different ZSM-5 catalys...

Methanol production from eucalyptus wood chips. Attachment VI. Florida's eucalyptus energy farm: the natural system interface

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fishkind, H.H.

1982-05-01

A review of pertinent literature covered the following: eucalypt background, the candidate species, biomass plantation considerations, effects of site production, leachate and allelopathy, and some exotic flora considerations. The comparative eucalypt field survey covers mined land stands, unmined south Florida stands, and Glade County eucalypt stands. The problem of eucalypt naturalization is discussed.

Moisture content of wood for interior use...Douglas-fir and robusta eucalyptus samples studied

Treesearch

R. Sidney Boone

1967-01-01

Panels of Douglas-fir and robusta eucalyptus blocks showed little seasonal variation in Equilibrium Moisture Content (EMC) of wood at 19 indoor locations on Oahu, Hawaii. Differences in EMC between locations were more variable. Minimum EMC at nonair-conditioned locations was 10 percent;at air-conditioned locations. 8 percent. Maximum EMC at nonairconditioned locations...

Evaluation of Stability and In Vitro Security of Nanoemulsions Containing Eucalyptus globulus Oil

PubMed Central

Quatrin, Priscilla Maciel; Sagrillo, Michele Rorato; Nascimento, Kátia

2017-01-01

Essential oil of Eucalyptus globulus presents several pharmacological properties. However, their therapeutic efficacy may be affected by limitations due to several conditions, rendering it difficult to obtain stable and effective pharmaceutical formulations. The use of nanotechnology is an alternative to improve their characteristics aiming to ensure their stability and effectiveness. Furthermore, studies about the possible toxic effects of nanostructures are necessary to evaluate safety when the formulation comes into contact with human cells. Hence, in this paper, we evaluate for the first time the stability and in vitro cytogenotoxicity of nanoemulsions containing Eucalyptus globulus in peripheral blood mononuclear cells. As a result, the stability study found that the best condition for storage up to 90 days was refrigeration (4°C); it was the condition that best preserved the nanometric features. The content of the major compounds of oil was maintained after nanoencapsulation and preserved over time. In tests to evaluate the safety of this formulation, we can conclude that, at a low concentration (approximately 0.1%), Eucalyptus globulus nanoemulsion did not cause toxicity in peripheral blood mononuclear cells and also showed a protective effect in cells against possible damage when compared to oil in free form. PMID:28691021

First Evidence of Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae) Feeding From Mesophyll of Eucalyptus Leaves.

PubMed

Santadino, Marina; Brentassi, María E; Fanello, Diego D; Coviella, Carlos

2017-04-01

The bronze bug, Thaumastocoris peregrinus Carpintero & Dellapé, 2006 (Heteroptera: Thaumastocoridae) originally restricted to Australia, is an important emerging pest of Eucalyptus plantations in the Southern Hemisphere. It feeds on mature leaves, causing the loss of photosynthetic surface area and defoliation and, according to some studies, even tree death. In this work, feeding activities of T. peregrinus on Eucalyptus camaldulensis Dehn leaves and its primary food resources were identified. In cross sections of leaves, solid salivary deposits on epidermal cells and in the vicinity of stomata cells were detected. In subepidermal cells of the palisade parenchyma near the stylet penetration point, disorganization and removal of chloroplasts were also observed. The presence of chlorophyll in guts of adults and nymphs was analyzed using spectrophotometry and confocal laser scanning to obtain in situ fluorescent spectra. Both spectra showed chlorophyll absorbance peaks. In addition, the presence of chlorophyll in guts of T. peregrinus using fluorescence microscopy was identified. These results provide the first evidence that T. peregrinus feeds from the palisade parenchyma (chlorenchyma) of Eucalyptus leaves. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Prognoses of diameter and height of trees of eucalyptus using artificial intelligence.

PubMed

Vieira, Giovanni Correia; de Mendonça, Adriano Ribeiro; da Silva, Gilson Fernandes; Zanetti, Sidney Sára; da Silva, Mayra Marques; Dos Santos, Alexandre Rosa

2018-04-01

Models of individual trees are composed of sub-models that generally estimate competition, mortality, and growth in height and diameter of each tree. They are usually adopted when we want more detailed information to estimate forest multiproduct. In these models, estimates of growth in diameter at 1.30m above the ground (DBH) and total height (H) are obtained by regression analysis. Recently, artificial intelligence techniques (AIT) have been used with satisfactory performance in forest measurement. Therefore, the objective of this study was to evaluate the performance of two AIT, artificial neural networks and adaptive neuro-fuzzy inference system, to estimate the growth in DBH and H of eucalyptus trees. We used data of continuous forest inventories of eucalyptus, with annual measurements of DBH, H, and the dominant height of trees of 398 plots, plus two qualitative variables: genetic material and site index. It was observed that the two AIT showed accuracy in growth estimation of DBH and H. Therefore, the two techniques discussed can be used for the prognosis of DBH and H in even-aged eucalyptus stands. The techniques used could also be adapted to other areas and forest species. Copyright © 2017 Elsevier B.V. All rights reserved.

Superior cottonwood and eucalyptus clones for biomass production in wastewater biomass production in wastewater bioremediation systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rockwood, D.L.; Pisano, S.M.; McConnell, W.V.

1996-12-31

Fast-growing cottonwood and Eucalyptus species have wastewater bioremediation potential. To estimate genetic variation in cottonwood`s response to sewage effluent, 10 clones were planted at Tallahassee in April 1992. Progenies and/or clones of E. Ampligolia (EA). E. Camaldulensis (EC), and E. Grandis (EG) were planted in a dry stormwater retention/bioremediation pond constructed in June 1993 at Tampa. Genetic variability within cottonwood and Eucalyptus species was observed and should be utilized to optimize biomass production and nutrient uptake in wastewater bioremediation applications. On good sites with freeze risk in northern Florida, three cottonwood clones are particularly productive. While as many as fourmore » EC and EG clones are promising, one EG clone appears superior for stormwater remediation, systems in central Florida.« less

Pectin Methylesterase Genes Influence Solid Wood Properties of Eucalyptus pilularis1[C][W][OA

PubMed Central

Sexton, Timothy R.; Henry, Robert J.; Harwood, Chris E.; Thomas, Dane S.; McManus, Luke J.; Raymond, Carolyn; Henson, Michael; Shepherd, Mervyn

2012-01-01

This association study of Eucalyptus pilularis populations provides empirical evidence for the role of Pectin Methylesterase (PME) in influencing solid wood characteristics of Eucalyptus. PME6 was primarily associated with the shrinkage and collapse of drying timber, which are phenotypic traits consistent with the role of pectin as a hydrophilic polysaccharide. PME7 was primarily associated with cellulose and pulp yield traits and had an inverse correlation with lignin content. Selection of specific alleles in these genes may be important for improving trees as sources of high-quality wood products. A heterozygote advantage was postulated for the PME7 loci and, in combination with haplotype blocks, may explain the absence of a homozygous class at all single-nucleotide polymorphisms investigated in this gene. PMID:22052017

A facile and green preparation of reduced graphene oxide using Eucalyptus leaf extract

NASA Astrophysics Data System (ADS)

Li, Chengyang; Zhuang, Zechao; Jin, Xiaoying; Chen, Zuliang

2017-11-01

In this paper, a green and facile synthesis of reduced graphene oxide (GO) by Eucalyptus leaf extract (EL-RGO) was investigated, which was characterized with ultraviolet-visible spectroscopy (UV), Raman spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and Thermal gravimetric analysis (TG). Eucalyptus leaf extract also play both reducing and capping stabilizing agents prepared EL-RGO as shown a good stability and electrochemical properties. This approach could provide an alternative method to prepare EL-RGO in large-scale production. Moreover, the good electrochemical property and biocompatibility can be used in various applications. In addition, the merit of this study is that both the oxidized products and the reducing agents are environmental friendly by green reduction.

Eucalyptus development in degraded soil fertilized with sewage sludge and mineral fertilizer

NASA Astrophysics Data System (ADS)

Rodrigues, R. A. F.; Santos, E. B.; Alves, M. C.; Arruda, O. G.

2012-04-01

The aim of this study was to compare the development of eucalyptus in a degraded Oxisol with mineral fertilizer and sewage sludge. The study was conducted in Selviria, Mato Grosso do Sul, Brasil. The culture of eucalyptus was planted in 2003 at 2.0 m x 1.5 m spacing, with application of 60 Mg ha-1 of sewage sludge (dry basis) and mineral fertilizer. After five years (2008) the area received biosolids and mineral fertilizer, and after five months, were evaluated for height and diameter at breast height of Eucalyptus. The experimental design was randomized blocks with four treatments: T1 - control (without addition of inputs), T2 - Mineral fertilization (30 kg ha-1 N, 90 kg ha-1 of P2O5 and 60 kg ha-1 K2O), T3 - Reapplication of 4.64 Mg ha-1 of sewage sludge, dry basis, T4 - Reapplication of 9.28 Mg ha-1 of sewage sludge, dry basis. Before reapplication the biosolids plant height was higher in the eucalyptus with treatment 9.28 Mg ha-1 of sewage sludge (8.03 m) compared to control (5.75 m) and mineral fertilizer (5.91 m) and that treatment 4.64 Mg ha-1 of sewage sludge (6.34 m) did not differ from the previous three. For the diameter at breast height was the highest value for treatment with 9.28 Mg ha-1 (7.78 cm) compared to control (5.23 cm) and 4.64 Mg ha-1 (5.03 cm), and that of mineral fertilizer (5.96 cm) did not differ from all treatments. After reapplication of sludge plant height was higher in the eucalyptus treatment with 9.28 Mg ha-1 of sewage sludge (11.21 m) compared with control (7.51 m), mineral fertilizer (7.77 m) and 4 64 Mg ha-1 (8.07 m), which did not differ. The diameter at breast height had the same behavior before the application of biosolids in the highest value observed being 9.28 Mg ha-1 (8.46 cm) compared with control (5.75 cm) and 4.64 Mg ha-1 (5.03 cm) and that of mineral fertilizer (6.34 cm) did not differ from the others. Reapplication of the dose of 9.28 Mg ha-1 of sewage sludge in degraded Oxisol provided greater height and diameter at breast height from eucalyptus trees.

Converging patterns of vertical variability in leaf morphology and nitrogen across seven Eucalyptus plantations in Brazil and Hawaii, USA

Treesearch

Adam P. Coble; Alisha Autio; Molly A. Cavaleri; Dan Binkley; Michael G. Ryan

2014-01-01

Across sites in Brazil and Hawaii, LMA and Nmass were strongly correlated with height and shade index, respectively, which may help simplify canopy function modeling of Eucalyptus plantations. Abstract Within tree canopies, leaf mass per area (LMA) and leaf nitrogen per unit area (Narea) commonly increase with height. Previous research has suggested that these patterns...

Equations for predicting biomass in 2- to 6-year-old Eucalyptus saligna in Hawaii

Treesearch

Craig D. Whitesell; Susan C. Miyasaka; Robert F. Strand; Thomas H. Schubert; Katharine E. McDuffie

1988-01-01

Eucalyptus saligna trees grown in short-rotation plantations on the island of Hawaii were measured, harvested, and weighed to provide data for developing regression equations using non-destructive stand measurements. Regression analysis of the data from 190 trees in the 2.0- to 3.5-year range and 96 trees in the 4- to 6-year range related stem-only...

Approaches to predicting current and future distributions of Puccinia psidii in South America under climate change scenarios

Treesearch

N. B. Klopfenstein; J. W. Hanna; R. N. Graca; A. L. Ross-Davis; P. G. Cannon; A. C. Alfenas; M. -S. Kim

2011-01-01

Puccinia psidii is the cause of Eucalyptus/guava/myrtle rust disease of many host species in the Myrtaceae family, including guava (Psidium spp.), eucalypt (Eucalyptus spp.), rose apple (Syzygium jambos), and ohia (Metrosideros polymorpha) (Farr and Rossman 2010). First reported in 1884 on guava in Brazil (Maclachlan 1938), the rust has since been detected in other...

Adequacy of different experimental designs for eucalyptus spacing trials in Portuguese environmental conditions

Treesearch

Paula Soares; Margarida Tome

2000-01-01

In Portugal, several eucalyptus spacing trials cover a relatively broad range of experimental designs: trials with a non-randomized block design with plots of different size and number of trees per plot; trials based on a non-systematic design in which spacings were randomized resulting in a factorial arrangement with plots of different size and shape and equal number...

Mapping aboveground carbon stocks using LiDAR data in Eucalyptus spp. plantations in the state of Sao Paulo, Brazil

Treesearch

Carlos Alberto Silva; Carine Klauberg; Samuel de Padua Chaves e Carvalho; Andrew T. Hudak; e Luiz Carlos Estraviz Rodriguez

2014-01-01

Fast growing plantation forests provide a low-cost means to sequester carbon for greenhouse gas abatement. The aim of this study was to evaluate airborne LiDAR (Light Detection And Ranging) to predict aboveground carbon (AGC) stocks in Eucalyptus spp. plantations. Biometric parameters (tree height (Ht) and diameter at breast height (DBH)) were collected from...

Physical and Mechanical Properties of Cellulose Nanofibril Films from Bleached Eucalyptus Pulp by Endoglucanase Treatment and Microfluidization

Treesearch

Wangxia Wang; Ronald C. Sabo; Michael D. Mozuch; Phil Kersten; J. Y. Zhu; Yongcan Jin

2015-01-01

A GH5 hyperthermostable endoglucanase (Ph-GH5) from the archaeon Pyrococcus horikoshii and a commercial endoglucanase (FR) were used to treat bleached eucalyptus pulp (BEP) fibers to produce cellulose nanofibrils (CNF) and subsequently to CNF films. TEM imaging indicated that Ph-GH5 produced longer and more entangled CNF than FR with the same number...

Variations in diameter measurements of Robusta Eucalyptus due to swelling and shrinking of bark

Treesearch

Robert E. Burgan

1971-01-01

Trunk diameters of Eucalyptus robusta trees shrink and swell as bark moisture content changes. Diameter variations from this cause as measured on six trees with a dial-gage dendrometer were less than 1 percent of trunk diameter. To compare this variation with the variation in d.b.h. measurements that can result from personal techiques of using a...

Stereo photo series for quantifying natural fuels.Volume XIII: grasslands, shrublands, oak-bay woodlands, and eucalyptus forests in the East Bay of California.

Treesearch

Clinton S. Wright; Robert E. Vihnanek

2014-01-01

Four series of photographs display a range of natural conditions and fuel loadings for grassland, shrubland, oak-bay woodland, and eucalyptus forest ecosystems on the eastern slopes of the San Francisco Bay area of California. Each group of photos includes inventory information summarizing vegetation composition, structure, and loading; woody material loading and...

Pest risk assessment of the importation into the United States of unprocessed Eucalyptus logs and chips from South America

Treesearch

John T. Kliejunas; Harold H. Burdsall; Gregg A. DeNitto; Andris Eglitis; Dennis A. Haugen; William E. Wallner

2001-01-01

In this report, we assess the unmitigated pest risk potential of importing Eucalyptus logs and chips from South America into the United States. To do this, we estimated the likelihood and consequences of introducing representative insects and pathogens of concern. Nineteen individual pest risk assessments were prepared, eleven dealing with insects and eight with...

Why don't our stands grow even faster? Control of production and carbon cycling in eucalypt plantations

Treesearch

M.G. Ryan; D. Binkley; J.L. Stape

2008-01-01

The growth of Eucalyptus stands varies several fold across sites, under the influence of resource availability, stand age and stand structure. We describe a series of related studies that aim to understand the mechanisms that drive this great range in stand growth rates. In a seven-year study in Hawaii of Eucalyptus saligna at a...

Growth and spacing in Eucalyptus-A case study from Punjab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kapur, S.K.; Dogra, A.S.

1992-12-31

A spacing trial of Eucalyptus tereticornis Smith involving 14 different spacings in a randomized block design with four replications was started in Punjab in 1988. Analysis of data 7 years after planting shows that a spacing of 3.0 m x 3.0 m may be best for pulpwood production, although there is no significant difference between different spacings in terms of volume produced.

Aromatic hydrocarbon production via eucalyptus urophylla pyrolysis over several metal modified ZSM-5 catalysts – an analysis by py-GC/MS

USDA-ARS?s Scientific Manuscript database

Metal modified HZSM-5 catalysts were prepared by ion exchange of NH4ZSM-5 (SIO2/Al2O3 = 23) using gallium, molybdenum, nickel and zinc, and their combinations thereof. The prepared catalysts were used to evaluate catalytic pyrolysis for the conversion of Eucalyptus urophylla to fuels and chemicals, ...

Two new species of sympatric Fergusonina flies (Diptera: Fergusoninidae) from bud galls on high elevation snow gums (Eucalyptus pauciflora Sieb. ex Spreng. complex) in the Australian Alps

USDA-ARS?s Scientific Manuscript database

Two species of Fergusonina Malloch fly, F. daviesae Nelson sp.n. and F. taylori Nelson sp.n. (Diptera: Fergusoninidae), are described from terminal leaf bud galls on high elevation snow gums (Eucalyptus pauciflora complex) in the Australian Alps. These species occur in sympatry at the six locations...

Acacia Changes Microbial Indicators and Increases C and N in Soil Organic Fractions in Intercropped Eucalyptus Plantations.

PubMed

Pereira, Arthur P A; Zagatto, Maurício R G; Brandani, Carolina B; Mescolotti, Denise de Lourdes; Cotta, Simone R; Gonçalves, José L M; Cardoso, Elke J B N

2018-01-01

Intercropping forest plantations of Eucalyptus with nitrogen-fixing trees can increase soil N inputs and stimulate soil organic matter (OM) cycling. However, microbial indicators and their correlation in specific fractions of soil OM are unclear in the tropical sandy soils. Here, we examined the microbial indicators associated with C and N in the soil resulting from pure and intercropped Eucalyptus grandis and Acacia mangium plantations. We hypothesized that introduction of A. mangium in a Eucalyptus plantation promotes changes in microbial indicators and increases C and N concentrations on labile fractions of the soil OM, when compared to pure eucalyptus plantations. We determined the microbial and enzymatic activity, and the potential for C degradation by the soil microbial community. Additionally, we evaluated soil OM fractions and litter parameters. Soil (0-20 cm) and litter samples were collected at 27 and 39 months after planting from the following treatments: pure E. grandis (E) and A. mangium (A) plantations, pure E. grandis plantations with N fertilizer (E+N) and an E. grandis , and A. mangium intercropped plantations (E+A). The results showed that intercropped plantations (E+A) increase 3, 45, and 70% microbial biomass C as compared to A, E+N, and E, at 27 months after planting. The metabolic quotient ( q CO 2 ) showed a tendency toward stressful values in pure E. grandis plantations and a strong correlation with dehydrogenase activity. A and E+A treatments also exhibited the highest organic fractions (OF) and C and N contents. A canonical redundancy analysis revealed positive correlations between microbial indicators of soil and litter attributes, and a strong effect of C and N variables in differentiating A and E+A from E and E+N treatments. The results suggested that a significant role of A. mangium enhance the dynamics of soil microbial indicators which help in the accumulation of C and N in soil OF in intercropped E. grandis plantations. Our results are mostly relevant to plantations in sandy soil areas with low levels of OM, suggesting and efficient method for improving nutrient availability in the soil and optimizing eucalyptus growth and development.

Acacia Changes Microbial Indicators and Increases C and N in Soil Organic Fractions in Intercropped Eucalyptus Plantations

PubMed Central

Pereira, Arthur P. A.; Zagatto, Maurício R. G.; Brandani, Carolina B.; Mescolotti, Denise de Lourdes; Cotta, Simone R.; Gonçalves, José L. M.; Cardoso, Elke J. B. N.

2018-01-01

Intercropping forest plantations of Eucalyptus with nitrogen-fixing trees can increase soil N inputs and stimulate soil organic matter (OM) cycling. However, microbial indicators and their correlation in specific fractions of soil OM are unclear in the tropical sandy soils. Here, we examined the microbial indicators associated with C and N in the soil resulting from pure and intercropped Eucalyptus grandis and Acacia mangium plantations. We hypothesized that introduction of A. mangium in a Eucalyptus plantation promotes changes in microbial indicators and increases C and N concentrations on labile fractions of the soil OM, when compared to pure eucalyptus plantations. We determined the microbial and enzymatic activity, and the potential for C degradation by the soil microbial community. Additionally, we evaluated soil OM fractions and litter parameters. Soil (0–20 cm) and litter samples were collected at 27 and 39 months after planting from the following treatments: pure E. grandis (E) and A. mangium (A) plantations, pure E. grandis plantations with N fertilizer (E+N) and an E. grandis, and A. mangium intercropped plantations (E+A). The results showed that intercropped plantations (E+A) increase 3, 45, and 70% microbial biomass C as compared to A, E+N, and E, at 27 months after planting. The metabolic quotient (qCO2) showed a tendency toward stressful values in pure E. grandis plantations and a strong correlation with dehydrogenase activity. A and E+A treatments also exhibited the highest organic fractions (OF) and C and N contents. A canonical redundancy analysis revealed positive correlations between microbial indicators of soil and litter attributes, and a strong effect of C and N variables in differentiating A and E+A from E and E+N treatments. The results suggested that a significant role of A. mangium enhance the dynamics of soil microbial indicators which help in the accumulation of C and N in soil OF in intercropped E. grandis plantations. Our results are mostly relevant to plantations in sandy soil areas with low levels of OM, suggesting and efficient method for improving nutrient availability in the soil and optimizing eucalyptus growth and development. PMID:29670606

High-throughput SNP genotyping in the highly heterozygous genome of Eucalyptus: assay success, polymorphism and transferability across species

PubMed Central

2011-01-01

Background High-throughput SNP genotyping has become an essential requirement for molecular breeding and population genomics studies in plant species. Large scale SNP developments have been reported for several mainstream crops. A growing interest now exists to expand the speed and resolution of genetic analysis to outbred species with highly heterozygous genomes. When nucleotide diversity is high, a refined diagnosis of the target SNP sequence context is needed to convert queried SNPs into high-quality genotypes using the Golden Gate Genotyping Technology (GGGT). This issue becomes exacerbated when attempting to transfer SNPs across species, a scarcely explored topic in plants, and likely to become significant for population genomics and inter specific breeding applications in less domesticated and less funded plant genera. Results We have successfully developed the first set of 768 SNPs assayed by the GGGT for the highly heterozygous genome of Eucalyptus from a mixed Sanger/454 database with 1,164,695 ESTs and the preliminary 4.5X draft genome sequence for E. grandis. A systematic assessment of in silico SNP filtering requirements showed that stringent constraints on the SNP surrounding sequences have a significant impact on SNP genotyping performance and polymorphism. SNP assay success was high for the 288 SNPs selected with more rigorous in silico constraints; 93% of them provided high quality genotype calls and 71% of them were polymorphic in a diverse panel of 96 individuals of five different species. SNP reliability was high across nine Eucalyptus species belonging to three sections within subgenus Symphomyrtus and still satisfactory across species of two additional subgenera, although polymorphism declined as phylogenetic distance increased. Conclusions This study indicates that the GGGT performs well both within and across species of Eucalyptus notwithstanding its nucleotide diversity ≥2%. The development of a much larger array of informative SNPs across multiple Eucalyptus species is feasible, although strongly dependent on having a representative and sufficiently deep collection of sequences from many individuals of each target species. A higher density SNP platform will be instrumental to undertake genome-wide phylogenetic and population genomics studies and to implement molecular breeding by Genomic Selection in Eucalyptus. PMID:21492434

In vitro anticancer properties of selected Eucalyptus species.

PubMed

Bhuyan, Deep Jyoti; Sakoff, Jennette; Bond, Danielle R; Predebon, Melanie; Vuong, Quan V; Chalmers, Anita C; van Altena, Ian A; Bowyer, Michael C; Scarlett, Christopher J

2017-08-01

In spite of the recent advancements in oncology, the overall survival rate for pancreatic cancer has not improved over the last five decades. Eucalypts have been linked with cytotoxic and anticancer properties in various studies; however, there is very little scientific evidence that supports the direct role of eucalypts in the treatment of pancreatic cancer. This study assessed the anticancer properties of aqueous and ethanolic extracts of four Eucalyptus species using an MTT assay. The most promising extracts were further evaluated using a CCK-8 assay. Apoptotic studies were performed using a caspase 3/7 assay in MIA PaCa-2 cells. The aqueous extract of Eucalyptus microcorys leaf and the ethanolic extract of Eucalyptus microcorys fruit inhibited the growth of glioblastoma, neuroblastoma, lung and pancreatic cancer cells by more than 80% at 100 μg/mL. The E. microcorys and Eucalyptus saligna extracts showed lower GI 50 values than the ethanolic Eucalyptus robusta extract in MIA PaCa-2 cells. Aqueous E. microcorys leaf and fruit extracts at 100 μg/mL exerted significantly higher cell growth inhibition in MIA PaCa-2 cells than other extracts (p < 0.05). Statistically similar IC 50 values (p > 0.05) were observed in aqueous E. microcorys leaf (86.05 ± 4.75 μg/mL) and fruit (64.66 ± 15.97 μg/mL) and ethanolic E. microcorys leaf (79.30 ± 29.45 μg/mL) extracts in MIA PaCa-2 cells using the CCK-8 assay. Caspase 3/7-mediated apoptosis and morphological changes of cells were also witnessed in MIA PaCa-2 cells after 24 h of treatment with the extracts. This study highlighted the significance of E. microcorys as an important source of phytochemicals with efficacy against pancreatic cancer cells. Further studies are warranted to purify and structurally identify individual compounds and elucidate their mechanisms of action for the development of more potent and specific chemotherapeutic agents for pancreatic cancer.

Dual RNA-Sequencing of Eucalyptus nitens during Phytophthora cinnamomi Challenge Reveals Pathogen and Host Factors Influencing Compatibility

PubMed Central

Meyer, Febé E.; Shuey, Louise S.; Naidoo, Sitha; Mamni, Thandekile; Berger, Dave K.; Myburg, Alexander A.; van den Berg, Noëlani; Naidoo, Sanushka

2016-01-01

Damage caused by Phytophthora cinnamomi Rands remains an important concern on forest tree species. The pathogen causes root and collar rot, stem cankers, and dieback of various economically important Eucalyptus spp. In South Africa, susceptible cold tolerant Eucalyptus plantations have been affected by various Phytophthora spp. with P. cinnamomi considered one of the most virulent. The molecular basis of this compatible interaction is poorly understood. In this study, susceptible Eucalyptus nitens plants were stem inoculated with P. cinnamomi and tissue was harvested five days post inoculation. Dual RNA-sequencing, a technique which allows the concurrent detection of both pathogen and host transcripts during infection, was performed. Approximately 1% of the reads mapped to the draft genome of P. cinnamomi while 78% of the reads mapped to the Eucalyptus grandis genome. The highest expressed P. cinnamomi gene in planta was a putative crinkler effector (CRN1). Phylogenetic analysis indicated the high similarity of this P. cinnamomi CRN1 to that of Phytophthora infestans. Some CRN effectors are known to target host nuclei to suppress defense. In the host, over 1400 genes were significantly differentially expressed in comparison to mock inoculated trees, including suites of pathogenesis related (PR) genes. In particular, a PR-9 peroxidase gene with a high similarity to a Carica papaya PR-9 ortholog previously shown to be suppressed upon infection by Phytophthora palmivora was down-regulated two-fold. This PR-9 gene may represent a cross-species effector target during P. cinnamomi infection. This study identified pathogenicity factors, potential manipulation targets, and attempted host defense mechanisms activated by E. nitens that contributed to the susceptible outcome of the interaction. PMID:26973660

Targeting oxidative stress, acetylcholinesterase, proinflammatory cytokine, dopamine and GABA by eucalyptus oil (Eucalyptus globulus) to alleviate ketamine-induced psychosis in rats.

PubMed

Yadav, Monu; Jindal, Deepak Kumar; Parle, Milind; Kumar, Anil; Dhingra, Sameer

2018-02-20

Essential oil of eucalyptus species is among the most common traded essential oils in the world. There is an increasing interest in the application of eucalyptus oil as a natural additive in food and pharmaceutical industry. The present study was undertaken to identify the phytoconstituents present in the essential oil of Eucalyptus globulus leaves (EO) and ascertain their protective effect against ketamine-induced psychosis in rats. GC-MS technique was used for analysis of phytoconstituents present in EO. Ketamine (50 mg/kg, i.p.) was used to induce psychosis in rats. Photoactometer, forced swim test and pole climb avoidance test were used to evaluate the protective effects of the EO (500, 1000 and 2000 mg/kg, p.o.) on acute and chronic administration. Bar test was used to test the side effect of EO. Biochemical and neurochemical estimations were carried out to explore the possible mechanism of action. GC-MS analysis of EO showed the presence of a number of biologically active compounds. EO at the dose of 500, 1000 and 2000 mg/kg, p.o. on acute and chronic administration, decreased locomotor activity, immobility duration and latency to climb the pole. EO was effective to facilitate the release of GABA, increase GSH levels, inhibit dopamine neurotransmission and decrease TNF-α levels as well as diminish AChE activity in different regions of the brain. EO at the dose of 500, 1000 mg/kg did not produce cataleptic behavior in rats. EO at the dose of 500, 1000 mg/kg produced protective effects against ketamine-induced psychosis and can be further explored clinically against neuropsychiatric disorders.

De novo assembled expressed gene catalog of a fast-growing Eucalyptus tree produced by Illumina mRNA-Seq

PubMed Central

2010-01-01

Background De novo assembly of transcript sequences produced by short-read DNA sequencing technologies offers a rapid approach to obtain expressed gene catalogs for non-model organisms. A draft genome sequence will be produced in 2010 for a Eucalyptus tree species (E. grandis) representing the most important hardwood fibre crop in the world. Genome annotation of this valuable woody plant and genetic dissection of its superior growth and productivity will be greatly facilitated by the availability of a comprehensive collection of expressed gene sequences from multiple tissues and organs. Results We present an extensive expressed gene catalog for a commercially grown E. grandis × E. urophylla hybrid clone constructed using only Illumina mRNA-Seq technology and de novo assembly. A total of 18,894 transcript-derived contigs, a large proportion of which represent full-length protein coding genes were assembled and annotated. Analysis of assembly quality, length and diversity show that this dataset represent the most comprehensive expressed gene catalog for any Eucalyptus tree. mRNA-Seq analysis furthermore allowed digital expression profiling of all of the assembled transcripts across diverse xylogenic and non-xylogenic tissues, which is invaluable for ascribing putative gene functions. Conclusions De novo assembly of Illumina mRNA-Seq reads is an efficient approach for transcriptome sequencing and profiling in Eucalyptus and other non-model organisms. The transcriptome resource (Eucspresso, http://eucspresso.bi.up.ac.za/) generated by this study will be of value for genomic analysis of woody biomass production in Eucalyptus and for comparative genomic analysis of growth and development in woody and herbaceous plants. PMID:21122097

Exhaust emissions reduction from diesel engine using combined Annona-Eucalyptus oil blends and antioxidant additive

NASA Astrophysics Data System (ADS)

Senthil, R.; Silambarasan, R.; Pranesh, G.

2017-03-01

The limited resources, rising petroleum prices and depletion of fossil fuel have now become a matter of great concern. Hence, there is an urgent need for researchers to find some alternate fuels which are capable of substituting partly or wholly the higher demanded conventional diesel fuel. Lot of research work has been conducted on diesel engine using biodiesel and its blends with diesel as an alternate fuel. Very few works have been done with combination of biodiesel-Eucalypts oil without neat diesel and this leads to lots of scope in this area. The aim of the present study is to analyze the performance and emission characteristics of a single cylinder, direct injection, compression ignition engine using eucalyptus oil-biodiesel as fuel. The presence of eucalyptus oil in the blend reduces the viscosity and improves the volatility of the blends. The methyl ester of Annona oil is blended with eucalypts oil in 10, 20, 30, 40 and 50 %. The performance and emission characteristics are evaluated by operating the engine at different loads. The performance characteristics such as brake thermal efficiency, brake specific fuel consumption and exhaust gas temperature are evaluated. The emission constituents measured are Carbon monoxide (CO), unburned hydrocarbons (HC), Oxides of nitrogen (NOx) and Smoke. It is found that A50-Eu50 (50 Annona + 50 % Eucalyptus oil) blend showed better performance and reduction in exhaust emissions. But, it showed a very marginal increase in NOx emission when compared to that of diesel. Therefore, in order to reduce the NOx emission, antioxidant additive (A-tocopherol acetate) is mixed with Annona-Eucalyptus oil blends in various proportions by which NOx emission is reduced. Hence, A50-Eu50 blend can be used as an alternate fuel for diesel engine without any modifications.

Is Eucalyptus Cryptically Self-incompatible?

PubMed

Horsley, Tasmien N; Johnson, Steven D

2007-12-01

The probability that seeds will be fertilized from self- versus cross-pollen depends strongly on whether plants have self-incompatibility systems, and how these systems influence the fate of pollen tubes. In this study of breeding systems in Eucalyptus urophylla and Eucalyptus grandis, epifluorescence microscopy was used to study pollen tube growth in styles following self- and cross-pollinations. Pollen tubes from self-pollen took significantly longer than those from cross-pollen to grow to the base of the style in both E. urophylla (120 h vs. 96 h) and E. grandis (96 h vs. 72 h). In addition, both species exhibited reduced seed yields following self-pollination compared with cross-pollination. The present observations suggest that, in addition to a late-acting self-incompatibility barrier, cryptic self-incompatibility could be a mechanism responsible for the preferential out-crossing system in these two eucalypt species.

Six-year growth of Eucalyptus saligna plantings as affected by nitrogen and phosphorous fertilizer

Treesearch

Craig D. Whitesell; Dean S. DeBell; Thomas H. Schubert

1987-01-01

Growth responses of Eucalyptus saligna to nitrogen (N) and phosphorus (P) fertilizers were assessed in bioenergy plantations on abandoned sugarcane land in Hawaii. Fertilizers were applied three times (0, 6, and 15 months after planting) in a factorial design with four dosages each of N (0, 25, 50, and 75 g urea per tree) and P (0, 30, 60, and 90 g...

Specific gravity variation in robusta eucalyptus grown in Hawaii

Treesearch

Roger G. Skolmen

1972-01-01

The specific gravity (air-dry volume, ovendry weight) of Eucalyptus robusta wood was tested within and between trees from 10 stands. Mean specific gravity was 0.603, but the range in individual samples for 50 trees was 0.331 to 0.869, and was 0.357 to 0.755 within one cross section. A consistent increase was recorded in all trees from pith to cambium and from butt to...

Eucalyptus production and the supply, use and efficiency of use of water, light and nitrogen across a geographic gradient in Brazil

Treesearch

Jose Luiz Stape; Dan Binkley; Michael G. Ryan

Millions of hectares of Eucalyptus are intensively managed for wood production in the tropics, but little is known about the physiological processes that control growth and their regulation. We examined the main environmental factors controlling growth and resource use across a geographic gradient with clonal E. grandis x urophylla in north-eastern Brazil. Rates of...

Endoglucanase post-milling treatment for producing cellulose nanofibers from bleached eucalyptus fibers by a supermasscolloider

Treesearch

Wangxia Wang; Michael D. Mozuch; Ronald C. Sabo; Philip Kersten; Junyong Zhu; Yongcan Jin

2016-01-01

Three recombinant GH5 endoglucanases chosen for their contrasting hydrolytic activities, and a commercial endoglucanase were used to treat cellulose nanofibers (CNFs) after they were milled from bleached eucalyptus pulp with a supermasscolloider. This enzyme ‘‘post-treatment’’ resulted in different properties for the CNFs depending on enzyme treatment. The degree of...

The Effect of Fertilization on Sap flux and Canopy Conductance in a Eucalyptus saligna Experimental Forest

Treesearch

R. Hubbard; M. Ryan; C. Giardina; H. Barnard

2004-01-01

Land devoted to plantation forestry (50 million ha) has been increasing worldwide the genus Eucalyptus is a popular plantation species (14 million ha) for its rapid growth and ability to grow well on a wide range of sites. Fertilization is a common silvicultural tool to improve tree growth with potential effects on stand water use, but relationship between wood growth...

Species diversity of Fergusonina Malloch gall flies (Diptera: Fergusoninidae) forming leaf bud galls on snow gum (Eucalyptus pauciflora Sieb. ex Spreng. complex), with a description of a new species from Tasmania

USDA-ARS?s Scientific Manuscript database

A new species of Fergusonina (Diptera: Fergusoninidae) fly is described from terminal leaf bud galls (TLBGs) from the Eucalyptus pauciflora Sieb. ex Spreng. (snow gum) species complex. Fergusonina tasmaniensis Nelson sp.n. is the first species from the genus Fergusonina to be described from Tasmania...

Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana

PubMed Central

Zwart, Lizahn; Berger, Dave Kenneth; Moleleki, Lucy Novungayo; van der Merwe, Nicolaas A.; Myburg, Alexander A.; Naidoo, Sanushka

2017-01-01

Eucalyptus species are cultivated for forestry and are of economic importance. The fungal stem canker pathogen Chrysoporthe austroafricana causes disease of varying severity on E. grandis. The Eucalyptus grandis-Chrysoporthe austroafricana interaction has been established as a model system for studying Eucalyptus antifungal defence. Previous studies revealed that the phytohormone salicylic acid (SA) affects the levels of resistance in highly susceptible (ZG14) and moderately resistant (TAG5) clones. The aims of this study were to examine histochemical changes in response to wounding and inoculation as well as host responses at the protein level. The anatomy and histochemical changes induced by wounding and inoculation were similar between the clones, suggesting that anatomical differences do not underlie their different levels of resistance. Tyloses and gum-like substances were present after inoculation and wounding, but cell death occurred only after inoculation. Hyphae of C. austroafricana were observed inside dead and living cells, suggesting that the possibility of a hemibiotrophic interaction requires further investigation. Proteomics analysis revealed the possible involvement of proteins associated with cell death, SA signalling and systemic resistance. In combination with previous information, this study forms a basis for future functional characterisation of candidate genes involved in resistance of E. grandis to C. austroafricana. PMID:28349984

Assessment of the salt tolerance and environmental biosafety of Eucalyptus camaldulensis harboring a mangrin transgene.

PubMed

Yu, Xiang; Kikuchi, Akira; Shimazaki, Takayoshi; Yamada, Akiyo; Ozeki, Yoshihiro; Matsunaga, Etsuko; Ebinuma, Hiroyasu; Watanabe, Kazuo N

2013-01-01

Increasing soil salinization of arable land has a major impact on the global ecosystem. One approach to increase the usable global forest area is to develop transgenic trees with higher tolerance to conditions of salt stress. An allene oxide cyclase homolog, mangrin, contains a core protein domain that enhances the salt tolerance of its host. We utilized this feature to develop improved salt-tolerant eucalyptus trees, by using transgenic Eucalyptus camaldulensis carrying the mangrin gene as a model. Since the Japanese government requires an environmental biosafety assessment for the surrounding biosphere, we performed experiments on trees grown in a special netted-house. This study examined the transgenic E. camaldulensis carrying the mangrin gene to assess the feasibility of using these transformants, and assessed their salt tolerance and environmental biosafety. We found that seven of 36 transgenic genotypes had significantly higher salt tolerance than non-transformants, and more importantly, that these plants had no significant impact on environmental biosafety. These results suggest that introduction of the mangrin gene may be one approach to safely enhance salt tolerance in genetically modified Eucalyptus species, and that the transformants have no apparent risks in terms of environmental biosafety. Thus, this study provides valuable information regarding the use of transgenic trees in situ.

Identification of a STOP1-like protein in Eucalyptus that regulates transcription of Al tolerance genes.

PubMed

Sawaki, Yoshiharu; Kobayashi, Yuriko; Kihara-Doi, Tomonori; Nishikubo, Nobuyuki; Kawazu, Tetsu; Kobayashi, Masatomo; Kobayashi, Yasufumi; Iuchi, Satoshi; Koyama, Hiroyuki; Sato, Shigeru

2014-06-01

Tolerance to soil acidity is an important trait for eucalyptus clones that are introduced to commercial forestry plantations in pacific Asian countries, where acidic soil is dominant in many locations. A conserved transcription factor regulating aluminum (Al) and proton (H⁺) tolerance in land-plant species, STOP1 (SENSITIVE TOPROTON RHIZOTOXICITY 1)-like protein, was isolated by polymerase chain reaction-based cloning, and then suppressed by RNA interference in hairy roots produced by Agrobacterium rhizogenes-mediated transformation. Eucalyptus STOP1-like protein complemented proton tolerance in an Arabidopsis thaliana stop1-mutant, and localized to the nucleus in a transient assay of a green fluorescent protein fusion protein expressed in tobacco leaves by Agrobacterium tumefaciens-mediated transformation. Genes encoding a citrate transporting MULTIDRUGS AND TOXIC COMPOUND EXTRUSION protein and an orthologue of ALUMINUM SENSITIVE 3 were suppressed in transgenic hairy roots in which the STOP1 orthologue was knocked down. In summary, we identified a series of genes for Al-tolerance in eucalyptus, including a gene for STOP1-like protein and the Al-tolerance genes it regulates. These genes may be useful for molecular breeding and genomic selection of elite clones to introduce into acid soil regions. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Potential of Eucalyptus Oil as Repellent against House Rat, Rattus rattus

PubMed Central

Thind, Ramandeep Kaur; Mahal, Amrit Kaur

2014-01-01

Rodent repellents are chemicals which by taste or odour or possibly by both will prevent animal from feeding or gnawing. Such substances may be used in protecting an area from rodent infestation or in protecting packaged food, packing materials, electric cables, and other important vulnerable materials. Mature and healthy house rat, Rattus rattus of both sexes, was exposed to 5, 10, and 20% eucalyptus oil applied as spray in laboratory pens in bichoice tests. Each concentration was applied through three different modes of application, that is, daily, once, and alternatively in a week. Repellent effect of the oil was assessed based on food consumption from treated and untreated sides for four days. In overall, food consumption was significantly (P < 0.0001) low from treatment side compared to the untreated side indicating significant repellent effect of the oil at all the three concentrations tested. Repellent effect of the oil was, however, not found to differ significantly between the two sexes. Percent repellency in both male and female rats was apparently more with daily application of 5 and 10% eucalyptus oil. Present studies reveal the potential of eucalyptus oil in repelling away R. rattus; however, further studies may be conducted to enhance the persistence of repellent effect for longer period of time. PMID:24523633

A model system to study the lignification process in Eucalyptus globulus.

PubMed

Araújo, Pedro; Cesarino, Igor; Mayer, Juliana Lischka Sampaio; Ferrari, Ilse Fernanda; Kiyota, Eduardo; Sawaya, Alexandra Christine Helena Frankland; Paes Leme, Adriana Franco; Mazzafera, Paulo

2014-09-01

Recalcitrance of plant biomass is closely related to the presence of the phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus is the main source of wood for pulp and paper industry. However, when compared to model plants such as Arabidopsis thaliana and poplar, relatively little is known about lignin biosynthesis in Eucalyptus and only a few genes were functionally characterized. An efficient, fast and inexpensive in vitro system was developed to study lignification in Eucalyptus globulus and to evaluate the potential role of candidate genes in this biological process. Seedlings were grown in four different conditions, in the presence or absence of light and with or without sucrose in the growth medium, and several aspects of lignin metabolism were evaluated. Our results showed that light and, to a lesser extent, sucrose induced lignin biosynthesis, which was followed by changes in S/G ratio, lignin oligomers accumulation and gene expression. In addition, higher total peroxidase activity and differential isoperoxidase profile were observed when seedlings were grown in the presence of light and sucrose. Peptide sequencing allowed the identification of differentially expressed peroxidases, which can be considered potential candidate class III peroxidases involved in lignin polymerization in E. globulus. © 2014 Scandinavian Plant Physiology Society.

Greater Soil Carbon Sequestration under Nitrogen-fixing Trees Compared with Eucalyptus Species.

Treesearch

Sigrid C. Resh; Dan Binkley

2002-01-01

Forests with nitrogen-fixing trees (N–fixers) typically accumulate more carbon (C) in soils than similar forests without N–fixing trees. This difference may develop from fundamentally different processes, with either greater accumulation of recently fixed C or reduced decomposition of older soil C. We compared the soil C pools under N–fixers with Eucalyptus (non–N–...

Productivity, nutrient cycling, and succession in single- and mixed-species plantations of Casuarina equisetifolia, Eucalyptus robusta, and Leucaena leucocephala in Puerto Rico

Treesearch

1999-01-01

Tree growth, biomass productivity, litterfall mass and nutrient content, changes in soil chemical properties and understory forest succession were evaluated over a 8.5-year period in single- and mixed-species (50 : 50) plantations of two N2-®xing species, Casuarina equisetifolia and Leucaena leucocephala, and a non-®xing species, Eucalyptus robusta. At the optimal...

Inter-laboratory comparisons of hexenuronic acid measurements in kraft eucalyptus pulps using a UV-Vis spectroscopic method

Treesearch

J.Y. Zhu; H.F Zhou; Chai X.S.; Donna Johannes; Richard Pope; Cristina Valls; M. Blanca Roncero

2014-01-01

An inter-laboratory comparison of a UV-Vis spectroscopic method (TAPPI T 282 om-13 “Hexeneuronic acid content of chemical pulp”) for hexeneuronic acid measurements was conducted using three eucalyptus kraft pulps. The pulp samples were produced in a laboratory at kappa numbers of approximately 14, 20, and 35. The hexeneuronic acid contents of the three pulps were...

Is Eucalyptus Cryptically Self-incompatible?

PubMed Central

Horsley, Tasmien N.; Johnson, Steven D.

2007-01-01

Background and Aims The probability that seeds will be fertilized from self- versus cross-pollen depends strongly on whether plants have self-incompatibility systems, and how these systems influence the fate of pollen tubes. Methods In this study of breeding systems in Eucalyptus urophylla and Eucalyptus grandis, epifluorescence microscopy was used to study pollen tube growth in styles following self- and cross-pollinations. Key Results Pollen tubes from self-pollen took significantly longer than those from cross-pollen to grow to the base of the style in both E. urophylla (120 h vs. 96 h) and E. grandis (96 h vs. 72 h). In addition, both species exhibited reduced seed yields following self-pollination compared with cross-pollination. Conclusions The present observations suggest that, in addition to a late-acting self-incompatibility barrier, cryptic self-incompatibility could be a mechanism responsible for the preferential out-crossing system in these two eucalypt species. PMID:17881341

The genome of Eucalyptus grandis.

PubMed

Myburg, Alexander A; Grattapaglia, Dario; Tuskan, Gerald A; Hellsten, Uffe; Hayes, Richard D; Grimwood, Jane; Jenkins, Jerry; Lindquist, Erika; Tice, Hope; Bauer, Diane; Goodstein, David M; Dubchak, Inna; Poliakov, Alexandre; Mizrachi, Eshchar; Kullan, Anand R K; Hussey, Steven G; Pinard, Desre; van der Merwe, Karen; Singh, Pooja; van Jaarsveld, Ida; Silva-Junior, Orzenil B; Togawa, Roberto C; Pappas, Marilia R; Faria, Danielle A; Sansaloni, Carolina P; Petroli, Cesar D; Yang, Xiaohan; Ranjan, Priya; Tschaplinski, Timothy J; Ye, Chu-Yu; Li, Ting; Sterck, Lieven; Vanneste, Kevin; Murat, Florent; Soler, Marçal; Clemente, Hélène San; Saidi, Naijib; Cassan-Wang, Hua; Dunand, Christophe; Hefer, Charles A; Bornberg-Bauer, Erich; Kersting, Anna R; Vining, Kelly; Amarasinghe, Vindhya; Ranik, Martin; Naithani, Sushma; Elser, Justin; Boyd, Alexander E; Liston, Aaron; Spatafora, Joseph W; Dharmwardhana, Palitha; Raja, Rajani; Sullivan, Christopher; Romanel, Elisson; Alves-Ferreira, Marcio; Külheim, Carsten; Foley, William; Carocha, Victor; Paiva, Jorge; Kudrna, David; Brommonschenkel, Sergio H; Pasquali, Giancarlo; Byrne, Margaret; Rigault, Philippe; Tibbits, Josquin; Spokevicius, Antanas; Jones, Rebecca C; Steane, Dorothy A; Vaillancourt, René E; Potts, Brad M; Joubert, Fourie; Barry, Kerrie; Pappas, Georgios J; Strauss, Steven H; Jaiswal, Pankaj; Grima-Pettenati, Jacqueline; Salse, Jérôme; Van de Peer, Yves; Rokhsar, Daniel S; Schmutz, Jeremy

2014-06-19

Eucalypts are the world's most widely planted hardwood trees. Their outstanding diversity, adaptability and growth have made them a global renewable resource of fibre and energy. We sequenced and assembled >94% of the 640-megabase genome of Eucalyptus grandis. Of 36,376 predicted protein-coding genes, 34% occur in tandem duplications, the largest proportion thus far in plant genomes. Eucalyptus also shows the highest diversity of genes for specialized metabolites such as terpenes that act as chemical defence and provide unique pharmaceutical oils. Genome sequencing of the E. grandis sister species E. globulus and a set of inbred E. grandis tree genomes reveals dynamic genome evolution and hotspots of inbreeding depression. The E. grandis genome is the first reference for the eudicot order Myrtales and is placed here sister to the eurosids. This resource expands our understanding of the unique biology of large woody perennials and provides a powerful tool to accelerate comparative biology, breeding and biotechnology.

Genetic divergence among Psidium accessions based on single nucleotide polymorphisms developed for Eucalyptus.

PubMed

Costa, S R; Santos, C A F

2017-05-04

The goal of this study was to analyze the genetic divergence among Psidium species accessions based on SNPs developed for Eucalyptus. Fifty-three Psidium accessions, including 47 P. guajava, were genotyped with EUCHIP60K. The dendrogram similarity ranged from 0.58 to 1.00, with a cophenetic value of 0.97. Five groups were identified at dendrogram cut point of 0.7: the first with 44 guava accessions, the second with 1 guava accession, the third with 3 P. guineense accessions, the forth with 2 guava accessions, and the fifth with 3 P. cattleianum accessions. The Bayesian analyses suggested seven subpopulations, with formation of two additional groups with guava accessions. Primers designed with Eucalyptus SNP sequences resulted in reliable Psidium amplicons on 6% polyacrylamide gels. In general, the SNP dendrogram agreed with biological genus structure, since different species were not grouped, indicating that transferability among Myrtaceae genus was possible and reliable.

Effects of aqueous eucalyptus extracts on seed germination, seedling growth and activities of peroxidase and polyphenoloxidase in three wheat cultivar seedlings (Triticum aestivum L.).

PubMed

Ziaebrahimi, L; Khavari-Nejad, R A; Fahimi, H; Nejadsatari, T

2007-10-01

Evaluation of allelopathic effects of this plant on other near cultivations especially wheat is the aim of this study. Effects of water extracts of eucalyptus leaves examined on germination and growth of three wheat cultivar seeds and seedlings. Results showed that: germination percentage strongly decreased, leaf and root lengths also affected and dry and wet weights of both roots and shoots showed similar change patterns. Activities of peroxidase and polyphenoloxidase as antioxidant enzymes in roots and shoots measured. Activity of peroxidases increased in stress conditions and roots showed more increased enzyme activity than leaves. Activity of polyphenoloxidases increased only in one of three cultivars and again roots showed more activity of this enzyme in response to eucalyptus extract. Suggest that detoxification process were conducted mainly in roots of seedlings.

Competition for light and light use efficiency for Acacia mangium and Eucalyptus grandis trees in mono-specific and mixed-species plantations in Brazil

NASA Astrophysics Data System (ADS)

Le Maire, G.; Nouvellon, Y.; Gonçalves, J.; Bouillet, J.; Laclau, J.

2010-12-01

Mixed plantations with N-fixing species might be an attractive option for limiting the use of fertilizer in highly productive Eucalyptus plantations. A randomized block design was set up in southern Brazil, including a replacement series and an additive series design, as well as a nitrogen fertilization treatment, and conducted during a full 6 years rotation. The gradient of competition between Eucalyptus and Acacia in this design resulted in very different conditions of growth of Acacia, from totally dominated up to dominant canopies. We used the MAESTRA model to estimate the amount of absorbed photosynthetically active radiation (APAR) at tree level. This model requires the description of the scene and distinct structural variables of the two species, and their evolution with time. The competition for light is analysed by comparing the inter-specific values of APAR during a period of 2 years at the end of the rotation. APAR is further compared to the measured increment in stem wood biomass of the tree, and their ratio is an estimation of the light use efficiency for stemwood production at tree-scale. Variability of these LUE are analysed in respect to the species, the size of the tree, and at plot scale (competition level). Stemwood production was 3400, 3900 and 2400 gDM/m2 while APAR was 1640, 2280 and 2900 MJ/y for the pure Eucalyptus, pure Acacia and 50/50 mixed plantation, respectively, for an average LAI of 3.7, 3.3 and 4.5, respectively. Individual LUE for stemwood was estimated at an average value of 1.72 and 1.41 gDM/MJ/tree for Eucalyptus and Acacia, respectively, and at 0.92 and 0.40 gDM/MJ/tree when they were planted in mixed 50/50 plantations. LUE was highly dependant on tree size for both species. At the plot scale, LUE for stemwood were 2.1 gDM/MJ and 1.75 for Eucalyptus and Acacias, respectively, and 0.85 for the mixed 50/50 plantation. These results suggest that the mixed 50/50 plantation, which absorbed a higher amount of light, produce less stemwood since half of the canopy (Acacias) are dominated, and the other half does not benefit much in terms of tree growth compared to absorbed light. The eventual benefit of the nitrogen-fixing species is not visible in the mixture with 50% of each species. More attention has to be paid to introducing acacias in an additive series with the same density of eucalyptus trees as in the monospecific stands.

Notes on the Occurrence of Oligonychus milleri (McGregor) and Oligonychus ununguis (Jacobi) (Acari: Tetranychidae) in Brazil.

PubMed

Castro, E B; Zanardi, O C; Garlet, J; Ochoa, R; Feres, R J F

2018-06-01

We verified infestation of Oligonychus milleri (McGregor) on plantations of Pinus caribaea (Pinaceae) and of Oligonychus ununguis (Jacobi) on plantations of Eucalyptus urophylla x Eucalyptus grandis (Myrtaceae) in State of Rondônia, Northern region of Brazil. This represents the first record of O. milleri in Brazil. Oligonychus ununguis was recorded previously, on cypress. The damage caused by these two spider mites in the plantations is described herein.

Growth of young saligna eucalyptus in Hawaii: 6 years after thinning

Treesearch

Craig D. Whitesell

1975-01-01

The effect of thinning on growth rate and quality of a 6-year-old Eucalyptus saligna Sm. stand on Maui, Hawaii, was studied by testing three levels of thinning from below: 100, 85, and 70 sq ft per acre (23, 19.5, and 16 sq m/ha). The stand had an average basal area of 102 sq ft per acre (23 sq m/ha). Initial spacing was about 10 by 10 feet. During...

Comparative interrogation of the developing xylem transcriptomes of two wood-forming species: Populus trichocarpa and Eucalyptus grandis.

PubMed

Hefer, Charles A; Mizrachi, Eshchar; Myburg, Alexander A; Douglas, Carl J; Mansfield, Shawn D

2015-06-01

Wood formation is a complex developmental process governed by genetic and environmental stimuli. Populus and Eucalyptus are fast-growing, high-yielding tree genera that represent ecologically and economically important species suitable for generating significant lignocellulosic biomass. Comparative analysis of the developing xylem and leaf transcriptomes of Populus trichocarpa and Eucalyptus grandis together with phylogenetic analyses identified clusters of homologous genes preferentially expressed during xylem formation in both species. A conserved set of 336 single gene pairs showed highly similar xylem preferential expression patterns, as well as evidence of high functional constraint. Individual members of multi-gene orthologous clusters known to be involved in secondary cell wall biosynthesis also showed conserved xylem expression profiles. However, species-specific expression as well as opposite (xylem versus leaf) expression patterns observed for a subset of genes suggest subtle differences in the transcriptional regulation important for xylem development in each species. Using sequence similarity and gene expression status, we identified functional homologs likely to be involved in xylem developmental and biosynthetic processes in Populus and Eucalyptus. Our study suggests that, while genes involved in secondary cell wall biosynthesis show high levels of gene expression conservation, differential regulation of some xylem development genes may give rise to unique xylem properties. © 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.

Oligo-carrageenan kappa-induced reducing redox status and activation of TRR/TRX system increase the level of indole-3-acetic acid, gibberellin A3 and trans-zeatin in Eucalyptus globulus trees.

PubMed

González, Alberto; Contreras, Rodrigo A; Zúiga, Gustavo; Moenne, Alejandra

2014-08-20

Eucalyptus globulus trees treated with oligo-carrageenan (OC) kappa showed an increase in NADPH, ascorbate and glutathione levels and activation of the thioredoxin reductase (TRR)/thioredoxin (TRX) system which enhance photosynthesis, basal metabolism and growth. In order to analyze whether the reducing redox status and the activation of thioredoxin reductase (TRR)/thioredoxin (TRX) increased the level of growth-promoting hormones, trees were treated with water (control), with OC kappa, or with inhibitors of ascorbate synthesis, lycorine, glutathione synthesis, buthionine sulfoximine (BSO), NADPH synthesis, CHS-828, and thioredoxin reductase activity, auranofine, and with OC kappa, and cultivated for four additional months. Eucalyptus trees treated with OC kappa showed an increase in the levels of the auxin indole 3-acetic acid (IAA), gibberellin A3 (GA3) and the cytokinin trans-zeatin (t-Z) as well as a decrease in the level of the brassinosteroid epi-brassinolide (EB). In addition, treatment with lycorine, BSO, CHS-828 and auranofine inhibited the increase in IAA, GA3 and t-Z as well as the decrease in EB levels. Thus, the reducing redox status and the activation of TRR/TRX system induced by OC kappa increased the levels of IAA, GA3 and t-Z levels determining, at least in part, the stimulation of growth in Eucalyptus trees.

Pollen analysis of Australian honey.

PubMed

Sniderman, J M Kale; Matley, Kia A; Haberle, Simon G; Cantrill, David J

2018-01-01

Pollen analysis is widely used to verify the geographic origin of honeys, but has never been employed in Australia. In this study, we analysed the pollen content of 173 unblended honey samples sourced from most of the commercial honey producing regions in southern Australia. Southern Australian vegetation is dominated by Eucalyptus (Myrtaceae) forests and, as expected, most Australian honeys are palynologically dominated by Eucalyptus, while other important components include Myrtaceae taxa such as Corymbia/Angophora and the tribe Leptospermeae; plus Brassicaceae, Echium, Macadamia, and Acacia. An important feature of the honeys is the number of Myrtaceae pollen morphotypes per sample, which is generally high (mean = 4.6) compared to honeys produced outside of Australia, including Eucalyptus honeys produced in the Mediterranean region, and honeys produced in South America, which has its own rich indigenous Myrtaceae flora. In the latter regions, the number of Myrtaceae morphotypes is apparently generally ≤2. A high number of Myrtaceae morphotypes may be a feasible criterion for authenticating the origin of Australian honeys, since most Australian honey is produced by honey bees mainly working indigenous floral resources. Myrtaceae morphotype diversity is a convenient melissopalynological measure that could be applied even where detailed knowledge of the pollen morphology of the many component genera and species is absent. Palynological criteria developed in Europe for authenticating Eucalyptus honeys should not be relied upon for Australian honeys, since those criteria are not based on samples of Australian honey.

Field performance of alternative landfill covers vegetated with cottonwood and eucalyptus trees.

PubMed

Abichou, Tarek; Musagasa, Jubily; Yuan, Lei; Chanton, Jeff; Tawfiq, Kamal; Rockwood, Donald; Licht, Louis

2012-01-01

A field study was conducted to assess the ability of landfill covers to control percolation into the waste. Performance of one conventional cover was compared to that of two evapotranspiration (ET) tree covers, using large (7 x 14 m) lined lysimeters at the Leon County Solid Waste management facility in Tallahassee, Florida. Additional unlined test sections were also constructed and monitored in order to compare soil water storage, soil temperature, and tree growth inside lysimeters and in unlined test sections. The unlined test sections were in direct contact with landfill gas. Surface runoff on the ET covers was a small proportion of the water balance (1% of precipitation) as compared to 13% in the conventional cover. Percolation in the ET covers averaged 17% and 24% of precipitation as compared to 33% in the conventional cover. On average, soil water storage was higher in the lined lysimeters (429 mm) compared to unlined test sections (408 mm). The average soil temperature in the lysimeters was lower than in the unlined test sections. The average tree height inside the lysimeters was not significantly lower (8.04 mfor eucalyptus and 7.11 mfor cottonwood) than outside (8.82 m for eucalyptus and 8.01 m for cottonwood). ET tree covers vegetated with cottonwood or eucalyptus are feasible for North Florida climate as an alternative to GCL covers.

Hydraulic Balance of a Eucalyptus urophylla Plantation in Response to Periodic Drought in Low Subtropical China

PubMed Central

Zhang, Zhenzhen; Zhao, Ping; McCarthy, Heather R.; Ouyang, Lei; Niu, Junfeng; Zhu, Liwei; Ni, Guangyan; Huang, Yuqing

2016-01-01

A clear understanding of hydraulic regulation in cultivated plants is crucial for addressing challenges to forest water cycling due to climate changes in low subtropical China. Experiments were conducted to determine the hydrologic balance of a Eucalyptus urophylla plantation in response to periodic drought. Trees displayed lower stomatal conductance (GS) and leaf water potentials (ΨL) during the dry periods. A decrease of 22.4% was found for the maximum reference GS (GS at D = 1 kPa; GSref-max). Accordingly, specific hydraulic conductivity (ks) decreased by 45.3 – 65.6% from the wet to the dry season, depending on the tree size. Fairly stable leaf stomatal conductance (gs) with decreasing ΨL (ΨL < -1.6 MPa) contributed to the high water-use efficiency (WUE) of this Eucalyptus species. Additionally, the lower stomatal sensitivity (-m = 0.53) in the dry season might also be responsible for the high WUE, since we found an anisohydric behavior that was associated with photosynthetically active radiation (Q0). Larger trees were found to use water more efficiently than small trees, due to the higher sensitivity of ks to decreasing ΨL. This was also verified by the decreasing leaf carbon isotope discrimination (Δ13C) with increasing tree diameter. However, further studies are needed to determine the universality of these results for other Eucalyptus species in this region. PMID:27725821

Genetic architecture of carbon isotope composition and growth in Eucalyptus across multiple environments.

PubMed

Bartholomé, Jérôme; Mabiala, André; Savelli, Bruno; Bert, Didier; Brendel, Oliver; Plomion, Christophe; Gion, Jean-Marc

2015-06-01

In the context of climate change, the water-use efficiency (WUE) of highly productive tree varieties, such as eucalypts, has become a major issue for breeding programmes. This study set out to dissect the genetic architecture of carbon isotope composition (δ(13) C), a proxy of WUE, across several environments. A family of Eucalyptus urophylla × E. grandis was planted in three trials and phenotyped for δ(13) C and growth traits. High-resolution genetic maps enabled us to target genomic regions underlying δ(13) C quantitative trait loci (QTLs) on the E. grandis genome. Of the 15 QTLs identified for δ(13) C, nine were stable across the environments and three displayed significant QTL-by-environment interaction, suggesting medium to high genetic determinism for this trait. Only one colocalization was found between growth and δ(13) C. Gene ontology (GO) term enrichment analysis suggested candidate genes related to foliar δ(13) C, including two involved in the regulation of stomatal movements. This study provides the first report of the genetic architecture of δ(13) C and its relation to growth in Eucalyptus. The low correlations found between the two traits at phenotypic and genetic levels suggest the possibility of improving the WUE of Eucalyptus varieties without having an impact on breeding for growth. © 2015 CIRAD. New Phytologist © 2015 New Phytologist Trust.

Effect of aluminum on metabolism of organic acids and chemical forms of aluminum in root tips of Eucalyptus camaldulensis Dehnh.

PubMed

Ikka, Takashi; Ogawa, Tsuyoshi; Li, Donghua; Hiradate, Syuntaro; Morita, Akio

2013-10-01

Eucalyptus (Eucalyptus camaldulensis) has relatively high resistance to aluminum (Al) toxicity than the various herbaceous plants and model plant species. To investigate Al-tolerance mechanism, the metabolism of organic acids and the chemical forms of Al in the target site (root tips) in Eucalyptus was investigated. To do this, 2-year old rooted cuttings of E. camaldulensis were cultivated in half-strength Hoagland solution (pH 4.0) containing Al (0, 0.25, 0.5, 1.0, 2.5 and 5.0mM) salts for 5weeks; growth was not affected at concentrations up to 2.5mM even with Al concentration reaching 6000μgg(-1) DW. In roots, the citrate content also increased with increasing Al application. Concurrently, the activities of aconitase and NADP(+)-isocitrate dehydrogenase, which catalyze the decomposition of citrate, decreased. On the other hand, the activity of citrate synthase was not affected at concentrations up to 2.5mM Al. (27)Al-NMR spectroscopic analyses were carried out where it was found that Al-citrate complexes were a major chemical form present in cell sap of root tips. These findings suggested that E. camaldulensis detoxifies Al by forming Al-citrate complexes, and that this is achieved through Al-induced citrate accumulation in root tips via suppression of the citrate decomposition pathway. Copyright © 2013 Elsevier Ltd. All rights reserved.

Emission factors of particulate matter, polycyclic aromatic hydrocarbons, and levoglucosan from wood combustion in south-central Chile.

PubMed

Jimenez, Jorge; Farias, Oscar; Quiroz, Roberto; Yañez, Jorge

2017-07-01

In south-central Chile, wood stoves have been identified as an important source of air pollution in populated areas. Eucalyptus (Eucalyptus globulus), Chilean oak (Nothofagus oblique), and mimosa (Acacia dealbata) were burned in a single-chamber slow-combustion wood stove at a controlled testing facility located at the University of Concepción, Chile. In each experiment, 2.7-3.1 kg of firewood were combusted while continuously monitoring temperature, exhaust gases, burn rate, and collecting particulate matter samples in Teflon filters under isokinetic conditions for polycyclic aromatic hydrocarbon and levoglucosan analyses. Mean particulate matter emission factors were 2.03, 4.06, and 3.84 g/kg dry wood for eucalyptus, oak, and mimosa, respectively. The emission factors were inversely correlated with combustion efficiency. The mean emission factors of the sums of 12 polycyclic aromatic hydrocarbons in particle phases were 1472.5, 2134.0, and 747.5 μg/kg for eucalyptus, oak, and mimosa, respectively. Fluoranthene, pyrene, benzo[a]anthracene, and chrysene were present in the particle phase in higher proportions compared with other polycyclic aromatic hydrocarbons that were analyzed. Mean levoglucosan emission factors were 854.9, 202.3, and 328.0 mg/kg for eucalyptus, oak, and mimosa, respectively. Since the emissions of particulate matter and other pollutants were inversely correlated with combustion efficiency, implementing more efficient technologies would help to reduce air pollutant emissions from wood combustion. Residential wood burning has been identified as a significant source of air pollution in populated areas. Local wood species are combusted for home cooking and heating, which releases several toxic air pollutants, including particulate matter, carbon monoxide, and polycyclic aromatic hydrocarbons. Air pollutant emissions depend on the type of wood and the technology and operational conditions of the wood stove. A better understanding of emissions from local wood species and wood stove performance would help to identify better biomass fuels and wood stove technologies in order to reduce air pollution from residential wood burning.

Fumigant activity of plant essential oils and components from garlic (Allium sativum) and clove bud (Eugenia caryophyllata) oils against the Japanese termite (Reticulitermes speratus Kolbe).

PubMed

Park, Il-Kwon; Shin, Sang-Chul

2005-06-01

Plant essential oils from 29 plant species were tested for their insecticidal activities against the Japanese termite, Reticulitermes speratus Kolbe, using a fumigation bioassay. Responses varied with plant material, exposure time, and concentration. Good insecticidal activity against the Japanese termite was achived with essential oils of Melaleuca dissitiflora, Melaleuca uncinata, Eucalyptus citriodora, Eucalyptus polybractea, Eucalyptus radiata, Eucalyptus dives, Eucalyptus globulus, Orixa japonica, Cinnamomum cassia, Allium cepa, Illicium verum, Evodia officinalis, Schizonepeta tenuifolia, Cacalia roborowskii, Juniperus chinensis var. horizontalis, Juniperus chinensis var. kaizuka, clove bud, and garlic applied at 7.6 microL/L of air. Over 90% mortality after 3 days was achieved with O. japonica essential oil at 3.5 microL/L of air. E. citriodora, C. cassia, A. cepa, I. verum, S. tenuifolia, C. roborowskii, clove bud, and garlic oils at 3.5 microL/L of air were highly toxic 1 day after treatment. At 2.0 microL/L of air concentration, essential oils of I. verum, C. roborowskik, S. tenuifolia, A. cepa, clove bud, and garlic gave 100% mortality within 2 days of treatment. Clove bud and garlic oils showed the most potent antitermitic activity among the plant essential oils. Garlic and clove bud oils produced 100% mortality at 0.5 microL/L of air, but this decreased to 42 and 67% after 3 days of treatment at 0.25 microL/L of air, respectively. Analysis by gas chromatography-mass spectrometry led to the identification of three major compounds from garlic oil and two from clove bud oils. These five compounds from two essential oils were tested individually for their insecticidal activities against Japanese termites. Responses varied with compound and dose. Diallyl trisulfide was the most toxic, followed by diallyl disulfide, eugenol, diallyl sulfide, and beta-caryophyllene. The essential oils described herein merit further study as potential fumigants for termite control.

Stomatal sensitivity to vapour pressure deficit relates to climate of origin in Eucalyptus species.

PubMed

Bourne, Aimee E; Haigh, Anthony M; Ellsworth, David S

2015-03-01

Selecting plantation species to balance water use and production requires accurate models for predicting how species will tolerate and respond to environmental conditions. Although interspecific variation in water use occurs, species-specific parameters are rarely incorporated into physiologically based models because often the appropriate species parameters are lacking. To determine the physiological control over water use in Eucalyptus, five stands of Eucalyptus species growing in a common garden were measured for sap flux rates and their stomatal response to vapour pressure deficit (D) was assessed. Maximal canopy conductance and whole-canopy stomatal sensitivity to D and reduced water availability were lower in species originating from more arid climates of origin than those from humid climates. Species from humid climates showed a larger decline in maximal sap flux density (JSmax) with reduced water availability, and a lower D at which stomatal closure occurred than species from more arid climates, implying larger sensitivity to water availability and D in these species. We observed significant (P < 0.05) correlations of species climate of origin with mean vessel diameter (R(2) = 0.90), stomatal sensitivity to D (R(2) = 0.83) and the size of the decline in JSmax to restricted water availability (R(2) = 0.94). Thus aridity of climate of origin appears to have a selective role in constraining water-use response among the five Eucalyptus plantation species. These relationships emphasize that within this congeneric group of species, climate aridity constrains water use. These relationships have implications for species choices for tree plantation success against drought-induced losses and the ability to manage Eucalyptus plantations against projected changes in water availability and evaporation in the future. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Valuation of pollinator forage services provided by Eucalyptus cladocalyx.

PubMed

de Lange, Willem J; Veldtman, Ruan; Allsopp, Mike H

2013-08-15

We assess the monetary value of forage provisioning services for honeybees as provided by an alien tree species in the Western Cape province of South Africa. Although Eucalyptus cladocalyx is not an officially declared invader, it is cleared on a regular basis along with other invasive Eucalyptus species such as Eucalyptus camaldulensis, and Eucalyptus conferruminata (which have been prioritised for eradication in South Africa). We present some of the trade-offs associated with the clearing of E. cladocalyx by means of a practical example that illustrates a situation where the benefits of the species to certain stakeholders could support the containment of the species in demarcated areas, while allowing clearing outside such areas. Given the absence of market prices for such forage provisioning services, the replacement cost is used to present the value of the loss in forage as provided by E. cladocalyx if the alien tree species is cleared along with invasive alien tree species. Two replacement scenarios formed the basis for our calculations. The first scenario was an artificial diet as replacement for the forage provisioning service, which yielded a direct cost estimate of US$7.5 m per year. The second was based on a Fynbos cultivation/restoration initiative aimed at substituting the forage provisioning service of E. cladocalyx, which yielded a direct cost of US$20.2 m per year. These figures provide estimates of the potential additional cost burden on the beekeeping industry if E. cladocalyx is completely eradicated from the Western Cape. The cost estimates should be balanced against the negative impacts of E. cladocalyx on ecosystem services in order to make an informed decision with regard to appropriate management strategies for this species. The findings therefore serve as useful inputs to balance trade-offs for alien species that are considered as beneficial to some, but harmful to other. Copyright © 2013 Elsevier Ltd. All rights reserved.

The effect of elevated [CO{sub 2}] on growth and photosynthesis of two eucalyptus species exposed to high temperatures and water deficits

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roden, J.S.; Ball, M.C.

1996-07-01

Two species of eucalyptus (Eucalyptus macrorhyncha and Eucalyptus rossii) were grown for 8 weeks in either ambient (350 {mu}L L{sup {minus}1}) or elevated (700 {mu}L L{sup {minus}1}) CO{sub 2} concentrations, either well watered or without water additions, and subjected to a daily, 3-h high-temperature (45{degrees}C, maximum) and high-light (1250 {mu}mol photons m{sup {minus}2} s{sup {minus}1}, maximum) stress period. Water-stressed seedlings of E. macrorhyncha had higher leaf water potentials when grown in elevated [CO{sub 2}]. Growth analysis indicated that increased [CO{sub 2}] may allow eucalyptus species to perform better during conditions of low soil moisture. A down-regulation of photosynthetic capacity wasmore » observed for seedlings grown in elevated [CO{sub 2}] when well watered but not when water stressed. Well-water seedlings grown in elevated [CO{sub 2}] had lower quantum efficiencies as measured by chlorophyll fluorescence (the ratio of variable to maximal chlorophyll fluorescence [F{sub v}/F{sub m}]) than seedlings grown in ambine [CO{sub 2}] during the high-temperature stress period. However, no significant differences in F{sub v}/F{sub m} were observed between CO{sub 2} treatments when water was withheld. The reductions in dark-adapted F{sub v}/F{sub m} for plants grown in elevated [CO{sub 2}] were not well correlated with increased xanthophyll cycle photoprotection. However, reductions in the F{sub v}/F{sub m} were correlated with increased levels of nonstructural carbohydrates. The reduction in quantum efficiencies for plants grown in elevated [CO{sub 2}] is discussed in the context of feedback inhibition of electron transport associated with starch accumulation and variation in sink strength. 48 refs., 8 figs., 2 figs.« less

Controlling the release of wood extractives into water bodies by selecting suitable eucalyptus species

NASA Astrophysics Data System (ADS)

Kilulya, K. F.; Msagati, T. A. M.; Mamba, B. B.; Ngila, J. C.; Bush, T.

Pulping industries are increasing worldwide as a result of the increase in the demand for pulp for cellulose derivatives and paper manufacturing. Due to the activities involved in pulping processes, different chemicals from raw materials (wood) and bleaching agents are released in pulp-mill effluent streams discharged into the environment and find their way into water bodies. Large quantities of water and chemicals used in pulping result in large amounts of wastewater with high concentrations of extractives such as unsaturated fatty acids, which are known to be toxic, and plant sterols which affect the development, growth and reproduction of aquatic organisms. This study was aimed at assessing the composition of extractives in two eucalyptus species used for pulp production in South Africa, in order to identify the suitable species with regard to extractive content. Samples from two eucalyptus plant species (Eucalyptus grandis and Eucalyptus dunnii) were collected from three sites and analysed for extractives by first extracting with water, followed by Soxhlet extraction using acetone. Compounds were identified and quantified using gas chromatography-mass spectrometry (GC-MS). Major classes of extractives identified were fatty acids (mainly hexadecanoic acid, 9,12-octadecadienoic, 9-octadecenoic and octadecanoic acids) and sterols (mainly β-sitosterol and stigmastanol). E. dunnii was found to contain higher amounts of the compounds compared to those found in E. grandis in all sampled sites. Principal component analysis (PCA) was performed and explained 92.9% of the total variation using three principal components. It was revealed that the percentage of fatty acids, which has a negative influence on both principal components 2 and 3, was responsible for the difference between the species. E. grandis, which was found to contain low amounts of extractives, was therefore found suitable for pulping with regard to minimal water usage and environment pollution.

Uncovering the defence responses of Eucalyptus to pests and pathogens in the genomics age.

PubMed

Naidoo, Sanushka; Külheim, Carsten; Zwart, Lizahn; Mangwanda, Ronishree; Oates, Caryn N; Visser, Erik A; Wilken, Febé E; Mamni, Thandekile B; Myburg, Alexander A

2014-09-01

Long-lived tree species are subject to attack by various pests and pathogens during their lifetime. This problem is exacerbated by climate change, which may increase the host range for pathogens and extend the period of infestation by pests. Plant defences may involve preformed barriers or induced resistance mechanisms based on recognition of the invader, complex signalling cascades, hormone signalling, activation of transcription factors and production of pathogenesis-related (PR) proteins with direct antimicrobial or anti-insect activity. Trees have evolved some unique defence mechanisms compared with well-studied model plants, which are mostly herbaceous annuals. The genome sequence of Eucalyptus grandis W. Hill ex Maiden has recently become available and provides a resource to extend our understanding of defence in large woody perennials. This review synthesizes existing knowledge of defence mechanisms in model plants and tree species and features mechanisms that may be important for defence in Eucalyptus, such as anatomical variants and the role of chemicals and proteins. Based on the E. grandis genome sequence, we have identified putative PR proteins based on sequence identity to the previously described plant PR proteins. Putative orthologues for PR-1, PR-2, PR-4, PR-5, PR-6, PR-7, PR-8, PR-9, PR-10, PR-12, PR-14, PR-15 and PR-17 have been identified and compared with their orthologues in Populus trichocarpa Torr. & A. Gray ex Hook and Arabidopsis thaliana (L.) Heynh. The survey of PR genes in Eucalyptus provides a first step in identifying defence gene targets that may be employed for protection of the species in future. Genomic resources available for Eucalyptus are discussed and approaches for improving resistance in these hardwood trees, earmarked as a bioenergy source in future, are considered. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fishkind, H.H.

The overall environmental impact of the eucalyptus to methanol energy system in Florida is assessed. The environmental impacts associated with the following steps of the process are considered: (1) the greenhouse and laboratory; (2) the eucalyptus plantation; (3) transporting the mature logs; (4) the hammermill; and (5) the methanol synthesis plant. Next, the environmental effects of methanol as an undiluted motor fuel, methanol as a gasoline blend, and gasoline as motor fuels are compared. Finally, the environmental effects of the eucalypt gasification/methanol synthesis system are compared to the coal liquefaction and conversion system.

Forestry inventory based on multistage sampling with probability proportional to size

NASA Technical Reports Server (NTRS)

Lee, D. C. L.; Hernandez, P., Jr.; Shimabukuro, Y. E.

1983-01-01

A multistage sampling technique, with probability proportional to size, is developed for a forest volume inventory using remote sensing data. The LANDSAT data, Panchromatic aerial photographs, and field data are collected. Based on age and homogeneity, pine and eucalyptus classes are identified. Selection of tertiary sampling units is made through aerial photographs to minimize field work. The sampling errors for eucalyptus and pine ranged from 8.34 to 21.89 percent and from 7.18 to 8.60 percent, respectively.

Potent HGF/c-Met axis inhibitors from Eucalyptus globulus: the coupling of phloroglucinol and sesquiterpenoid is essential for the activity.

PubMed

Yang, Sheng-Ping; Zhang, Xiao-Wei; Ai, Jing; Gan, Li-She; Xu, Jin-Biao; Wang, Ying; Su, Zu-Shang; Wang, Lu; Ding, Jian; Geng, Mei-Yu; Yue, Jian-Min

2012-09-27

Eucalyptin A (1), together with two known compounds 2 and 3 exhibiting potent inhibition on HGF/c-Met axis, was discovered from the fruits of Eucalyptus globulus. 1 possessed an unprecedented carbon framework of phloroglucinol-coupled sesquiterpenoid, and its structure was elucidated by spectroscopic method and ECD calculation. A brief structure-activity relationship discussion indicated that the coupling of a phloroglucinol and a sesquiterpenoid is essential for the activity.

Energy Product Options for Eucalyptus Species Grown as Short Rotation Woody Crops

PubMed Central

Rockwood, Donald L.; Rudie, Alan W.; Ralph, Sally A.; Zhu, J.Y.; Winandy, Jerrold E.

2008-01-01

Eucalyptus species are native to Australia but grown extensively worldwide as short rotation hardwoods for a variety of products and as ornamentals. We describe their general importance with specific emphasis on existing and emerging markets as energy products and the potential to maximize their productivity as short rotation woody crops. Using experience in Florida USA and similar locations, we document their current energy applications and assess their productivity as short-term and likely long-term energy and related products. PMID:19325808

Contribution of botanical origin and sugar composition of honeys on the crystallization phenomenon.

PubMed

Escuredo, Olga; Dobre, Irina; Fernández-González, María; Seijo, M Carmen

2014-04-15

The present work provides information regarding the statistical relationships among the palynological characteristics, sugars (fructose, glucose, sucrose, melezitose and maltose), moisture content and sugar ratios (F+G, F/G and G/W) of 136 different honey types (including bramble, chestnut, eucalyptus, heather, acacia, lime, rape, sunflower and honeydew). Results of the statistical analyses (multiple comparison Bonferroni test, Spearman rank correlations and principal components) revealed the valuable significance of the botanical origin on the sugar ratios (F+G, F/G and G/W). Brassica napus and Helianthus annuus pollen were the variables situated near F+G and G/W ratio, while Castanea sativa, Rubus and Eucalyptus pollen were located further away, as shown in the principal component analysis. The F/G ratio of sunflower, rape and lime honeys were lower than those found for the chestnut, eucalyptus, heather, acacia and honeydew honeys (>1.4). A lower value F/G ratio and lower water content were related with a faster crystallization in the honey. Copyright © 2013 Elsevier Ltd. All rights reserved.

Evaluation of fire severity via analysis of photosynthetic pigments: Oak, eucalyptus and cork oak leaves in a Mediterranean forest.

PubMed

Soler, M; Úbeda, X

2018-01-15

Few studies to date have examined the effect of the high temperatures attained during wildfire events on the pigments present in forest foliage. Here, we seek to analyse the main photosynthetic pigments in the leaves of the oak, cork oak and eucalyptus following a wildfire. We also subject leaves of these last two species to a range of contact temperatures (100-500 °C) in the laboratory using a muffle furnace. The samples were left in the muffle for two hours at 100, 150, 200, 250, 300, 350, 400 and 500 °C, in line with other soil study models (Úbeda et al., 2009; Düdaite et al., 2013). At temperatures above 250 °C, chromatography fails to detect any pigments. A minimal increase in temperature degrades chlorophyll, the process being more rapid in eucalyptus than in cork oak, while it increases pheophytin concentrations. Copyright © 2017 Elsevier Ltd. All rights reserved.

Characterization of white poplar and eucalyptus after ionic liquid pretreatment as a function of biomass loading using X-ray diffraction and small angle neutron scattering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yuan, Xueming; Duan, Yonghao; He, Lilin

A systematic study was done to understand interactions among biomass loading during ionic liquid (IL) pretreatment, biomass type and biomass structures. White poplar and eucalyptus samples were pretreated using 1-ethyl-3-methylimidazolium acetate (EmimOAc) at 110 °C for 3 h at biomass loadings of 5, 10, 15, 20 and 25 wt%. All of the samples were chemically characterized and tested for enzymatic hydrolysis. Physical structures including biomass crystallinity and porosity were measured by X-ray diffraction (XRD) and small angle neutron scattering (SANS), respectively. SANS detected pores of radii ranging from ~25 to 625 Å, enabling assessment of contributions of pores with different sizes to increased porositymore » after pretreatment. Contrasting dependences of sugar conversion on white poplar and eucalyptus as a function of biomass loading were observed and cellulose crystalline structure was found to play an important role.« less

Physiological studies in young Eucalyptus stands in southern India and their use in estimating forest transpiration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roberts, J.M.; Rosier, P.T.W.; Murthy, K.V.

1992-12-31

Stomatal conductance, leaf water potential and leaf area index were measured in adjacent plantations of Eucalyptus camaldulensis and Eucalyptus tereticornis at Puradal, near Shimoga, Karnataka, southern India. The data were collected in a range of climatic conditions during a two year period immediately following plantation establishment. Physiological differences between the two species were small and confined largely to leaf area index. Stomatal conductance was highest in the post-monsoon period and declined to minimum values immediately prior to the onset of the monsoon, with the lowest conductances observed after the plantations had been established for more than one year. Stomatal conductance,more » leaf area index and above-canopy meteorological data were combined in a multi-layer transpiration model and used to calculate hourly values of transpiration from the two species. Rates of transpiration up to 6 mm d{sup {minus}1} were estimated for the post-monsoon period but fell to below 1 mm d{sup {minus}1} prior to the monsoon.« less

Curaua and eucalyptus nanofibers films by continuous casting: Mechanical and thermal properties.

PubMed

Claro, Pedro Ivo Cunha; Corrêa, Ana Carolina; de Campos, Adriana; Rodrigues, Vanessa Bolzan; Luchesi, Bruno Ribeiro; Silva, Luiz Eduardo; Mattoso, Luiz Henrique Capparelli; Marconcini, José Manoel

2018-02-01

A wide variety of new green materials such as curaua leaf fibers (CLFs) has potential applications in nanotechnology. This study aims to investigate the thermomechanical properties and morphological structure of cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs) films obtained by continuous casting. The CNCs were obtained by acid hydrolysis and CNFs by mechanical shearing from bleached CLFs and eucalyptus pulp. The morphology after continuous casting resulted in oriented nanofibers, and as a consequence there was mechanical anisotropy. CNCs films showed the greatest values of tensile strength (36±4MPa) and the more effective fibrillation provided better mechanical strength of eucalyptus CNFs films than curaua CNFs films. Sulfur groups and mechanical shear degradation affected the stability of CNCs and CNFs films, respectively. Thus, the type of nanostructure, the way they interact to each other, the cellulose source and the process interfere significantly on the properties of the films. Copyright © 2017 Elsevier Ltd. All rights reserved.

Characterization of white poplar and eucalyptus after ionic liquid pretreatment as a function of biomass loading using X-ray diffraction and small angle neutron scattering.

PubMed

Yuan, Xueming; Duan, Yonghao; He, Lilin; Singh, Seema; Simmons, Blake; Cheng, Gang

2017-05-01

A systematic study was performed to understand interactions among biomass loading during ionic liquid (IL) pretreatment, biomass type and biomass structures. White poplar and eucalyptus samples were pretreated using 1-ethyl-3-methylimidazolium acetate (EmimOAc) at 110°C for 3h at biomass loadings of 5, 10, 15, 20 and 25wt%. All of the samples were chemically characterized and tested for enzymatic hydrolysis. Physical structures including biomass crystallinity and porosity were measured by X-ray diffraction (XRD) and small angle neutron scattering (SANS), respectively. SANS detected pores of radii ranging from ∼25 to 625Å, enabling assessment of contributions of pores with different sizes to increased porosity after pretreatment. Contrasting dependences of sugar conversion on white poplar and eucalyptus as a function of biomass loading were observed and cellulose crystalline structure was found to play an important role. Copyright © 2017 Elsevier Ltd. All rights reserved.

Inhaled peppermint, rosemary and eucalyptus essential oils do not change spirometry in healthy individuals.

PubMed

Köteles, Ferenc; Babulka, Péter; Szemerszky, Renáta; Dömötör, Zsuzsanna; Boros, Szilvia

2018-06-18

Essential oils of herbal origin are widely used in the treatment of diseases of the upper and lower respiratory tract primarily due to their antibacterial and antiviral effects. Menthol, the major component of the essential oil of mint (Mentha) species, exhibits antispasmodic activity, which might result in improved lung function. In a randomized experiment, 106 healthy participants received nebulized peppermint, eucalyptus, or rosemary essential oil for 15 min or no treatment (control). None of the essential oils had an impact on the measured spirometric variables (forced vital capacity, peak expiratory flow, the ratio of the volume of air forcibly blown out in the first second to forced vital capacity). Participants' expectations regarding the effects of essential oils did not affect their objective performance, however, they predicted perceived (subjective) changes. Perceived and measured changes were not connected with each other. In conclusion, inhaled rosemary, peppermint, and eucalyptus essential oils' subjective (perceived) effect on spirometry is mediated at least partly by expectations. Copyright © 2018 Elsevier Inc. All rights reserved.

Predicted stand volume for Eucalyptus plantations by spatial analysis

NASA Astrophysics Data System (ADS)

Latifah, Siti; Teodoro, RV; Myrna, GC; Nathaniel, CB; Leonardo, M. F.

2018-03-01

The main objective of the present study was to assess nonlinear models generated by integrating the stand volume growth rate to estimate the growth and yield of Eucalyptus. The primary data was done for point of interest (POI) of permanent sample plots (PSPs) and inventory sample plots, in Aek Nauli sector, Simalungun regency,North Sumatera Province,Indonesia. from December 2008- March 2009. Today,the demand for forestry information has continued to grow over recent years. Because many forest managers and decision makers face complex decisions, reliable information has become the necessity. In the assessment of natural resources including plantation forests have been widely used geospatial technology.The yield of Eucalyptus plantations represented by merchantable volume as dependent variable while factors affecting yield namely stands variables and the geographic variables as independent variables. The majority of the areas in the study site has stand volume class 0 - 50 m3/ha with 16.59 ha or 65.85 % of the total study site.

Eucalyptus oil-loaded microcapsules grafted to cotton fabrics for acaricidal effect against Dermatophagoides farinae.

PubMed

Kim, Joo Ran

2017-05-01

The purpose of this study was to develop acaricidal cotton fabrics grafted with eucalyptus oil-loaded microcapsules (EOMCs) produced from green resources. EOMCs showed a broad size distribution between 0.5 and 6.5 μm, and had the average diameter 1.8 μm. EOMCs exhibited nonporous spherical shapes and individually remained on cotton fibres. Through AATCC mortality tests against house dust mites (HDMs) (Dermatophagoides farinae), the treated cotton fabric containing EOMCs resulted in 98.7% mortality. Acaricidal efficiency was due to the large amount of oxygenated monoterpene, 1,8-eucalyptol (75.8%) and hydroxylated monoterpenes such as α-terpineol (3.6%), terpinen-4-ol (0.4%) and linalool (0.3%) found in eucalyptus oil. These compounds are effective in enhancing penetration into HDMs. EOMCs produced from safe and natural sources can serve as a replacement for synthetic acaricides in controlling the population of HDM, leading to positive impacts on the human health and environment.

Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.

PubMed

Ali, Akbar; Khan, M Masroor A; Uddin, Moin; Naeem, M; Idrees, Mohd; Hashmi, Nadeem; Dar, Tariq Ahmad; Varshney, Lalit

2014-11-04

Eucalyptus citriodora Hook. is highly valued for its citronellal-rich essential oil (EO) extracted from its leaves. Hence, escalated EO production of eucalyptus is the need of hour. Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA). A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora. The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1). The treatment 6 (spray of ISA at 120 mg L(-1)) showed the highest value for most of the parameters studied. It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control. Copyright © 2014 Elsevier Ltd. All rights reserved.

Color reduction of sulfonated eucalyptus kraft lignin.

PubMed

Zhang, Hui; Bai, Youcan; Zhou, Wanpeng; Chen, Fangeng

2017-04-01

Several eucalyptus lignins named as HSL, SML and BSL were prepared by high temperature sulfonation, sulfomethylation, butane sultone sulfonation respectively. The color properties of samples were investigated. Under optimized conditions the sulfonic group (SO 3 H) content of HSL, SML and BSL reached 1.52, 1.60 and 1.58mmol/g, respectively. Samples were characterized by UV-vis spectroscopy, FTIR spectroscopy, 1 H NMR spectroscopy, GPC and brightness test, respectively. The results revealed that BSL performed a higher molecular weight and lighter color due to the phenolic hydroxyl blocking by 1,4-butane sultone (1,4-BS). The color reduction of sodium borohydride treated BSL (labeled as SBSL) was further enhanced and the brightness value was improved by 76.1% compared with the darkest HSL. SBSL process was much better than HSL and SML process. Hydroxyl blocking effect of 1,4-BS and reducibility of sodium borohydride played important roles in the color reduction of sulfonated eucalyptus kraft lignin. Copyright © 2017 Elsevier B.V. All rights reserved.

Structure of bird communities in eucalyptus plantations: nestedness as a pattern of species distribution.

PubMed

Jacoboski, L I; Mendonça-Lima, A de; Hartz, S M

2016-04-19

Replacement of native habitats by tree plantations has increased dramatically in Brazil, resulting in loss of structural components for birds, such as appropriate substrates for foraging and nesting. Tree plantations can also reduce faunal richness and change the composition of bird species. This study evaluated the structure of avian communities in eucalyptus plantations of different ages and in a native forest. We classified species as habitat specialists or generalists, and assessed if the species found in eucalyptus plantations are a subset of the species that occur in the native forest. Forty-one sampling sites were evaluated, with three point counts each, in a native forest and in eucalyptus plantations of four different ages. A total of 71 bird species were identified. Species richness and abundance were higher in the native forest, reflecting the greater heterogeneity of the habitat. The composition of bird species also differed between the native forest and plantations. The species recorded in the plantations represented a subset of the species of the native forest, with a predominance of generalist species. These species are more tolerant of habitat changes and are able to use the plantations. The commercial plantations studied here can serve as a main or occasional habitat for these generalists, especially for those that are semi-dependent on edge and forest. The bird species most affected by silviculture are those that are typical of open grasslands, and those that are highly dependent on well-preserved forests.

Biochar feedstock and pyrolysis temperature effects on leachate: DOC characteristics and nitrate losses from a Brazilian Cerrado Arenosol mixed with agricultural waste biochars.

PubMed

Speratti, Alicia B; Johnson, Mark S; Sousa, Heiriane Martins; Dalmagro, Higo J; Couto, Eduardo Guimarães

2018-04-01

Dissolved organic carbon (DOC) leached from Brazilian Cerrado Arenosols can lead to carbon (C) losses and lower soil fertility, while excessive nutrient, e.g. nitrate (NO 3 - ), leaching can potentially cause water contamination. As biochar has been shown to stabilize C and retain soil nutrients, a greenhouse experiment was conducted to test different biochars' contributions to DOC and NO 3 - leaching from a sandy soil. Biochars were made from four local agricultural waste feedstocks (cotton residue, swine manure, eucalyptus sawmill residue, sugarcane filtercake) pyrolysed at 400, 500 and 600 °C. Biochar was mixed with soil at 5% weight in pots and maize seeds planted. Leachate was collected weekly for six weeks and analyzed for DOC and NO 3 - concentrations, while fluorescence spectroscopy with parallel factor analysis (PARAFAC) was used to interpret DOC characteristics. Cotton and swine manure biochar treatments had higher DOC and NO 3 - losses than eucalyptus biochar, filtercake biochar, and control treatments. Cotton and swine manure biochar treatments at high temperatures lost mostly terrestrial, humified DOC, while swine manure, filtercake, and eucalyptus biochars at low temperatures lost mostly labile, microbially-derived DOC. Through the practical use of fluorescence spectroscopy, our study identified filtercake and eucalyptus biochars as most promising for retaining DOC and NO 3 - in a Cerrado Arenosol, potentially reducing stable C and nutrient losses. Copyright © 2018 Elsevier Ltd. All rights reserved.

Intercropped Silviculture Systems, a Key to Achieving Soil Fungal Community Management in Eucalyptus Plantations

PubMed Central

Rachid, Caio T. C. C.; Balieiro, Fabiano C.; Fonseca, Eduardo S.; Peixoto, Raquel Silva; Chaer, Guilherme M.; Tiedje, James M.; Rosado, Alexandre S.

2015-01-01

Fungi are ubiquitous and important contributors to soil nutrient cycling, playing a vital role in C, N and P turnover, with many fungi having direct beneficial relationships with plants. However, the factors that modulate the soil fungal community are poorly understood. We studied the degree to which the composition of tree species affected the soil fungal community structure and diversity by pyrosequencing the 28S rRNA gene in soil DNA. We were also interested in whether intercropping (mixed plantation of two plant species) could be used to select fungal species. More than 50,000 high quality sequences were analyzed from three treatments: monoculture of Eucalyptus; monoculture of Acacia mangium; and a mixed plantation with both species sampled 2 and 3 years after planting. We found that the plant type had a major effect on the soil fungal community structure, with 75% of the sequences from the Eucalyptus soil belonging to Basidiomycota and 19% to Ascomycota, and the Acacia soil having a sequence distribution of 28% and 62%, respectively. The intercropping of Acacia mangium in a Eucalyptus plantation significantly increased the number of fungal genera and the diversity indices and introduced or increased the frequency of several genera that were not found in the monoculture cultivation samples. Our results suggest that management of soil fungi is possible by manipulating the composition of the plant community, and intercropped systems can be a means to achieve that. PMID:25706388

FT overexpression induces precocious flowering and normal reproductive development in Eucalyptus.

PubMed

Klocko, Amy L; Ma, Cathleen; Robertson, Sarah; Esfandiari, Elahe; Nilsson, Ove; Strauss, Steven H

2016-02-01

Eucalyptus trees are among the most important species for industrial forestry worldwide. However, as with most forest trees, flowering does not begin for one to several years after planting which can limit the rate of conventional and molecular breeding. To speed flowering, we transformed a Eucalyptus grandis × urophylla hybrid (SP7) with a variety of constructs that enable overexpression of FLOWERING LOCUS T (FT). We found that FT expression led to very early flowering, with events showing floral buds within 1-5 months of transplanting to the glasshouse. The most rapid flowering was observed when the cauliflower mosaic virus 35S promoter was used to drive the Arabidopsis thaliana FT gene (AtFT). Early flowering was also observed with AtFT overexpression from a 409S ubiquitin promoter and under heat induction conditions with Populus trichocarpa FT1 (PtFT1) under control of a heat-shock promoter. Early flowering trees grew robustly, but exhibited a highly branched phenotype compared to the strong apical dominance of nonflowering transgenic and control trees. AtFT-induced flowers were morphologically normal and produced viable pollen grains and viable self- and cross-pollinated seeds. Many self-seedlings inherited AtFT and flowered early. FT overexpression-induced flowering in Eucalyptus may be a valuable means for accelerating breeding and genetic studies as the transgene can be easily segregated away in progeny, restoring normal growth and form. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

Intercropped silviculture systems, a key to achieving soil fungal community management in eucalyptus plantations.

PubMed

Rachid, Caio T C C; Balieiro, Fabiano C; Fonseca, Eduardo S; Peixoto, Raquel Silva; Chaer, Guilherme M; Tiedje, James M; Rosado, Alexandre S

2015-01-01

Fungi are ubiquitous and important contributors to soil nutrient cycling, playing a vital role in C, N and P turnover, with many fungi having direct beneficial relationships with plants. However, the factors that modulate the soil fungal community are poorly understood. We studied the degree to which the composition of tree species affected the soil fungal community structure and diversity by pyrosequencing the 28S rRNA gene in soil DNA. We were also interested in whether intercropping (mixed plantation of two plant species) could be used to select fungal species. More than 50,000 high quality sequences were analyzed from three treatments: monoculture of Eucalyptus; monoculture of Acacia mangium; and a mixed plantation with both species sampled 2 and 3 years after planting. We found that the plant type had a major effect on the soil fungal community structure, with 75% of the sequences from the Eucalyptus soil belonging to Basidiomycota and 19% to Ascomycota, and the Acacia soil having a sequence distribution of 28% and 62%, respectively. The intercropping of Acacia mangium in a Eucalyptus plantation significantly increased the number of fungal genera and the diversity indices and introduced or increased the frequency of several genera that were not found in the monoculture cultivation samples. Our results suggest that management of soil fungi is possible by manipulating the composition of the plant community, and intercropped systems can be a means to achieve that.

Intercropped silviculture systems, a key to achieving soil fungal community management in eucalyptus plantations

DOE PAGES

Caio T.C.C. Rachid; Balieiro, Fabiano C.; Fonseca, Eduardo S.; ...

2015-02-23

Fungi are ubiquitous and important contributors to soil nutrient cycling, playing a vital role in C, N and P turnover, with many fungi having direct beneficial relationships with plants. However, the factors that modulate the soil fungal community are poorly understood. We studied the degree to which the composition of tree species affected the soil fungal community structure and diversity by pyrosequencing the 28S rRNA gene in soil DNA. We were also interested in whether intercropping (mixed plantation of two plant species) could be used to select fungal species. More than 50,000 high quality sequences were analyzed from three treatments:more » monoculture of Eucalyptus; monoculture of Acacia mangium; and a mixed plantation with both species sampled 2 and 3 years after planting. We found that the plant type had a major effect on the soil fungal community structure, with 75% of the sequences from the Eucalyptus soil belonging to Basidiomycota and 19% to Ascomycota, and the Acacia soil having a sequence distribution of 28% and 62%, respectively. The intercropping of Acacia mangium in a Eucalyptus plantation significantly increased the number of fungal genera and the diversity indices and introduced or increased the frequency of several genera that were not found in the monoculture cultivation samples. Our results suggest that management of soil fungi is possible by manipulating the composition of the plant community, and intercropped systems can be a means to achieve that.« less

The Eucalyptus grandis R2R3-MYB transcription factor family: evidence for woody growth-related evolution and function.

PubMed

Soler, Marçal; Camargo, Eduardo Leal Oliveira; Carocha, Victor; Cassan-Wang, Hua; San Clemente, Hélène; Savelli, Bruno; Hefer, Charles A; Paiva, Jorge A Pinto; Myburg, Alexander A; Grima-Pettenati, Jacqueline

2015-06-01

The R2R3-MYB family, one of the largest transcription factor families in higher plants, controls a wide variety of plant-specific processes including, notably, phenylpropanoid metabolism and secondary cell wall formation. We performed a genome-wide analysis of this superfamily in Eucalyptus, one of the most planted hardwood trees world-wide. A total of 141 predicted R2R3-MYB sequences identified in the Eucalyptus grandis genome sequence were subjected to comparative phylogenetic analyses with Arabidopsis thaliana, Oryza sativa, Populus trichocarpa and Vitis vinifera. We analysed features such as gene structure, conserved motifs and genome location. Transcript abundance patterns were assessed by RNAseq and validated by high-throughput quantitative PCR. We found some R2R3-MYB subgroups with expanded membership in E. grandis, V. vinifera and P. trichocarpa, and others preferentially found in woody species, suggesting diversification of specific functions in woody plants. By contrast, subgroups containing key genes regulating lignin biosynthesis and secondary cell wall formation are more conserved across all of the species analysed. In Eucalyptus, R2R3-MYB tandem gene duplications seem to disproportionately affect woody-preferential and woody-expanded subgroups. Interestingly, some of the genes belonging to woody-preferential subgroups show higher expression in the cambial region, suggesting a putative role in the regulation of secondary growth. © 2014 The Authors New Phytologist © 2014 New Phytologist Trust.

The least-squares mixing models to generate fraction images derived from remote sensing multispectral data

NASA Technical Reports Server (NTRS)

Shimabukuro, Yosio Edemir; Smith, James A.

1991-01-01

Constrained-least-squares and weighted-least-squares mixing models for generating fraction images derived from remote sensing multispectral data are presented. An experiment considering three components within the pixels-eucalyptus, soil (understory), and shade-was performed. The generated fraction images for shade (shade image) derived from these two methods were compared by considering the performance and computer time. The derived shade images are related to the observed variation in forest structure, i.e., the fraction of inferred shade in the pixel is related to different eucalyptus ages.

Consequences to precipitation regimes of replacing native Brazilian Cerrado by managed Eucalyptus

NASA Astrophysics Data System (ADS)

Siqueira, M. B.; Mota, F. C. M.

2016-12-01

Before the large people influx and development of the central part of Brazil in the sixties, due to new capital Brasília, Cerrado, a typical Brazilian savanna-type vegetation, used to occupy about 2 million km2, going from the Amazon tropical forest, in the north, to the edges of what used to be the Atlantic forest in the southeast. Today, somewhat 50% of this area has given place to agriculture, pasture and managed forests. Understanding how Cerrado interacts with the atmosphere and how this interaction will be modified with this land-use change is a crucial step towards improving predictions of future climate-change scenarios. Cerrado is a vegetation adapted to a climate characterized by two distinct seasons, a wet season (Nov-Mar) and dry season (May-Ago), with April and October being transitions between seasons. Typically, 75% of precipitation happens in the wet-season months and only 5% during dry-season. In this study, it is investigated the potential impacts of the substitution of Cerrado to the eco-hydrological characteristics of the region. The focus here is Eucalyptus plantation, which has increased substantially in the last decade due to government incentives. Two eddy-covariance (EC) systems were installed, one in an undisturbed Cerrado Stricto area and other in a recently-established Eucalyptus plantation. The two areas are 1,400m apart and are subjected to the same meteorology and similar edaphic conditions. Besides instrumentation typical of EC towers, a soil-moisture profiling system was installed in each site. Surface flux data from the eddy-covariance towers, along with daily upper air sounding profiles collected at the Brasília International Airport (located 10Km from the towers), were used to drive a simplified Atmospheric Boundary Layer (ABL) slab model in order to investigate possible consequences of a large-scale shift from Cerrado to Eucalyptus to precipitation regimes in the region. Preliminary results show that replacing Cerrado for Eucalyptus causes reduction in ABL temperature, increase in ABL water-vapor mixing ratio and slightly reduces ABL height. Consequences of those changes on the hydrology could be most important in the season transition stages.

Carbon assimilation in Eucalyptus urophylla grown under high atmospheric CO2 concentrations: A proteomics perspective.

PubMed

Santos, Bruna Marques Dos; Balbuena, Tiago Santana

2017-01-06

Photosynthetic organisms may be drastically affected by the future climate projections of a considerable increase in CO 2 concentrations. Growth under a high concentration of CO 2 could stimulate carbon assimilation-especially in C3-type plants. We used a proteomics approach to test the hypothesis of an increase in the abundance of the enzymes involved in carbon assimilation in Eucalyptus urophylla plants grown under conditions of high atmospheric CO 2 . Our strategy allowed the profiling of all Calvin-Benson cycle enzymes and associated protein species. Among the 816 isolated proteins, those involved in carbon fixation were found to be the most abundant ones. An increase in the abundance of six key enzymes out of the eleven core enzymes involved in carbon fixation was detected in plants grown at a high CO 2 concentration. Proteome changes were corroborated by the detection of a decrease in the stomatal aperture and in the vascular bundle area in Eucalyptus urophylla plantlets grown in an environment of high atmospheric CO 2 . Our proteomics approach indicates a positive metabolic response regarding carbon fixation in a CO 2 -enriched atmosphere. The slight but significant increase in the abundance of the Calvin enzymes suggests that stomatal closure did not prevent an increase in the carbon assimilation rates. The sample enrichment strategy and data analysis used here enabled the identification of all enzymes and most protein isoforms involved in the Calvin-Benson-Bessham cycle in Eucalyptus urophylla. Upon growth in CO 2 -enriched chambers, Eucalyptus urophylla plantlets responded by reducing the vascular bundle area and stomatal aperture size and by increasing the abundance of six of the eleven core enzymes involved in carbon fixation. Our proteome approach provides an estimate on how a commercially important C3-type plant would respond to an increase in CO 2 concentrations. Additionally, confirmation at the protein level of the predicted genes involved in carbon assimilation may be used in plant transformation strategies aiming to increase plant adaptability to climate changes or to increase plant productivity. Copyright © 2016 Elsevier B.V. All rights reserved.

RNA-Seq reveals genotype-specific molecular responses to water deficit in eucalyptus

PubMed Central

2011-01-01

Background In a context of climate change, phenotypic plasticity provides long-lived species, such as trees, with the means to adapt to environmental variations occurring within a single generation. In eucalyptus plantations, water availability is a key factor limiting productivity. However, the molecular mechanisms underlying the adaptation of eucalyptus to water shortage remain unclear. In this study, we compared the molecular responses of two commercial eucalyptus hybrids during the dry season. Both hybrids differ in productivity when grown under water deficit. Results Pyrosequencing of RNA extracted from shoot apices provided extensive transcriptome coverage - a catalog of 129,993 unigenes (49,748 contigs and 80,245 singletons) was generated from 398 million base pairs, or 1.14 million reads. The pyrosequencing data enriched considerably existing Eucalyptus EST collections, adding 36,985 unigenes not previously represented. Digital analysis of read abundance in 14,460 contigs identified 1,280 that were differentially expressed between the two genotypes, 155 contigs showing differential expression between treatments (irrigated vs. non irrigated conditions during the dry season), and 274 contigs with significant genotype-by-treatment interaction. The more productive genotype displayed a larger set of genes responding to water stress. Moreover, stress signal transduction seemed to involve different pathways in the two genotypes, suggesting that water shortage induces distinct cellular stress cascades. Similarly, the response of functional proteins also varied widely between genotypes: the most productive genotype decreased expression of genes related to photosystem, transport and secondary metabolism, whereas genes related to primary metabolism and cell organisation were over-expressed. Conclusions For the most productive genotype, the ability to express a broader set of genes in response to water availability appears to be a key characteristic in the maintenance of biomass growth during the dry season. Its strategy may involve a decrease of photosynthetic activity during the dry season associated with resources reallocation through major changes in the expression of primary metabolism associated genes. Further efforts will be needed to assess the adaptive nature of the genes highlighted in this study. PMID:22047139

Overland flow generation processes in sub-humid Mediterranean forest stands

NASA Astrophysics Data System (ADS)

Ferreira, A. J. D.; Ferreira, C. S. S.; Coelho, C. O. A.; Walsh, R. P. D.; Shakesby, R. A.

2012-04-01

Forest soils in north and central Portugal have suffered and continue to suffer major structural changes as a result of forest management techniques, such as clear-felling and as a result of wildfire and rip-ploughing, which is carried out to prepare the ground for planting tree seedlings. In soils that have undergone these changes, the characteristics tend to be different for coniferous plantations, where the root system tends to die when the trees are cut following fire and subsequently may be consumed by fire to form a macropore network, and other types of tree plantations where the root system remains alive and allows regrowth from the sawn tree stumps. Overland flow thresholds decrease sharply as a result of rip-ploughing and forest fires and increase following clear-felling. The time taken for trees to reach maturity after wildfire differs markedly betwen the two main species (Pinus pinaster Aiton and Eucalyptus globulus Labill.) stands. In this paper, overland flow is considered in relation to rainfall, throughfall and throughflow, both in terms of hydrology and hydrochemistry in an attempt to understand overland flow generation mechanisms for a variety of forest land uses (mature pine and eucalyptus, pine seedling regrowth and eucalyptus regrowth from tree stumps, eucalyptus plantations and burned pine). Overland flow generation processes change sharply, even within a single rainfall event, as reflected in the soil hydrological processes and the hydrochemical fingerprints. These effects result from the different contact times for water and soil, which cause differences in the absorption and exhudation processes for the two species

Variations of DNA methylation in Eucalyptus urophylla×Eucalyptus grandis shoot tips and apical meristems of different physiological ages.

PubMed

Mankessi, François; Saya, Aubin R; Favreau, Bénédicte; Doulbeau, Sylvie; Conéjéro, Geneviève; Lartaud, Marc; Verdeil, Jean-Luc; Monteuuis, Olivier

2011-10-01

Global DNA methylation was assessed by high-performance liquid chromatography (HPLC) for the first time in Eucalyptus urophylla×Eucalyptus grandis shoot tips comparing three outdoor and one in vitro sources of related genotypes differing in their physiological age. The DNA methylation levels found were consistent with those reported for other Angiosperms using the same HPLC technology. Notwithstanding noticeable time-related fluctuations within each source of plant material, methylation rate was overall higher for the mature clone (13.7%) than for the rejuvenated line of the same clone (12.6%) and for the juvenile offspring seedlings (11.8%). The in vitro microshoots of the mature clone were less methylated (11.3%) than the other outdoor origins, but the difference with the juvenile seedlings was not significant. Immunofluorescence investigations on shoot apices established that the mature source could be distinguished from the rejuvenated and juvenile origins by a higher density of cells with methylated nuclei in leaf primordia. Shoot apical meristems (SAMs) from the mature clone also showed a greater proportion and more methylated cells than SAMs from the rejuvenated and juvenile origins. The nuclei of these latter were characterized by fewer and more dispersed labeled spots than for the mature source. Our findings establish that physiological ageing induced quantitative and qualitative variations of DNA methylation at shoot tip, SAM and even cellular levels. Overall this DNA methylation increased with maturation and conversely decreased with rejuvenation to reach the lower scores and to show the immunolabeling patterns that characterized juvenile material nuclei. Copyright © Physiologia Plantarum 2011.

Comparison of Ant Community Diversity and Functional Group Composition Associated to Land Use Change in a Seasonally Dry Oak Forest.

PubMed

Cuautle, M; Vergara, C H; Badano, E I

2016-04-01

Ants have been used to assess land use conversion, because they reflect environmental change, and their response to these changes have been useful in the identification of bioindicators. We evaluated ant diversity and composition associated to different land use change in a temperate forest (above 2000 m asl) in Mexico. The study was carried out in "Flor del Bosque" Park a vegetation mosaic of native Oak Forests and introduced Eucalyptus and grasslands. Species richness, dominance and diversity rarefaction curves, based on ant morphospecies and functional groups, were constructed and compared among the three vegetation types, for the rainy and the dry seasons of 2008-2009. Jaccard and Sorensen incidence-based indices were calculated to obtain similarity values among all the habitats. The Oak Forest was a rich dominant community, both in species and functional groups; the Eucalyptus plantation was diverse with low dominance. The most seasonality habitat was the grassland, with low species and high functional group diversity during the dry seasons, but the reverse pattern during the wet season. The Oak Forest was more similar to the Eucalyptus plantation than to the grassland, particularly during the dry season. Oak Forests are dominated by Cold Climate Specialists, specifically Prenolepis imparis (Say). The Eucalyptus and the grassland are characterized by generalized Myrmicinae, as Pheidole spp. and Monomorium ebenium (Forel). The conservation of the native Oak Forest is primordial for the maintenance of Cold Climate Specialist ant communities. The microclimatic conditions in this forest, probably, prevented the invasion by opportunistic species.

Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus.

PubMed

Li, Fagen; Zhou, Changpin; Weng, Qijie; Li, Mei; Yu, Xiaoli; Guo, Yong; Wang, Yu; Zhang, Xiaohong; Gan, Siming

2015-01-01

Dense genetic maps, along with quantitative trait loci (QTLs) detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR), expressed sequence tag (EST) derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS), and diversity arrays technology (DArT) markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus) and with the E. grandis genome sequence. Fifty-three QTLs for growth (10-56 months of age) and wood density (56 months) were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa.

Identification of PEG-induced water stress responsive transcripts using co-expression network in Eucalyptus grandis.

PubMed

Ghosh Dasgupta, Modhumita; Dharanishanthi, Veeramuthu

2017-09-05

Ecophysiological studies in Eucalyptus have shown that water is the principal factor limiting stem growth. Effect of water deficit conditions on physiological and biochemical parameters has been extensively reported in Eucalyptus. The present study was conducted to identify major polyethylene glycol induced water stress responsive transcripts in Eucalyptus grandis using gene co-expression network. A customized array representing 3359 water stress responsive genes was designed to document their expression in leaves of E. grandis cuttings subjected to -0.225MPa of PEG treatment. The differentially expressed transcripts were documented and significantly co-expressed transcripts were used for construction of network. The co-expression network was constructed with 915 nodes and 3454 edges with degree ranging from 2 to 45. Ninety four GO categories and 117 functional pathways were identified in the network. MCODE analysis generated 27 modules and module 6 with 479 nodes and 1005 edges was identified as the biologically relevant network. The major water responsive transcripts represented in the module included dehydrin, osmotin, LEA protein, expansin, arabinogalactans, heat shock proteins, major facilitator proteins, ARM repeat proteins, raffinose synthase, tonoplast intrinsic protein and transcription factors like DREB2A, ARF9, AGL24, UNE12, WLIM1 and MYB66, MYB70, MYB 55, MYB 16 and MYB 103. The coordinated analysis of gene expression patterns and coexpression networks developed in this study identified an array of transcripts that may regulate PEG induced water stress responses in E. grandis. Copyright © 2017 Elsevier B.V. All rights reserved.

Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus

PubMed Central

Weng, Qijie; Li, Mei; Yu, Xiaoli; Guo, Yong; Wang, Yu; Zhang, Xiaohong; Gan, Siming

2015-01-01

Dense genetic maps, along with quantitative trait loci (QTLs) detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR), expressed sequence tag (EST) derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS), and diversity arrays technology (DArT) markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus) and with the E. grandis genome sequence. Fifty-three QTLs for growth (10–56 months of age) and wood density (56 months) were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa. PMID:26695430

Fuzzy logic applied to prospecting for areas for installation of wood panel industries.

PubMed

Dos Santos, Alexandre Rosa; Paterlini, Ewerthon Mattos; Fiedler, Nilton Cesar; Ribeiro, Carlos Antonio Alvares Soares; Lorenzon, Alexandre Simões; Domingues, Getulio Fonseca; Marcatti, Gustavo Eduardo; de Castro, Nero Lemos Martins; Teixeira, Thaisa Ribeiro; Dos Santos, Gleissy Mary Amaral Dino Alves; Juvanhol, Ronie Silva; Branco, Elvis Ricardo Figueira; Mota, Pedro Henrique Santos; da Silva, Lilianne Gomes; Pirovani, Daiani Bernardo; de Jesus, Waldir Cintra; Santos, Ana Carolina de Albuquerque; Leite, Helio Garcia; Iwakiri, Setsuo

2017-05-15

Prospecting for suitable areas for forestry operations, where the objective is a reduction in production and transportation costs, as well as the maximization of profits and available resources, constitutes an optimization problem. However, fuzzy logic is an alternative method for solving this problem. In the context of prospecting for suitable areas for the installation of wood panel industries, we propose applying fuzzy logic analysis for simulating the planting of different species and eucalyptus hybrids in Espírito Santo State, Brazil. The necessary methodological steps for this study are as follows: a) agriclimatological zoning of different species and eucalyptus hybrids; b) the selection of the vector variables; c) the application of the Euclidean distance to the vector variables; d) the application of fuzzy logic to matrix variables of the Euclidean distance; and e) the application of overlap fuzzy logic to locate areas for installation of wood panel industries. Among all the species and hybrids, Corymbia citriodora showed the highest percentage values for the combined very good and good classes, with 8.60%, followed by Eucalyptus grandis with 8.52%, Eucalyptus urophylla with 8.35% and Urograndis with 8.34%. The fuzzy logic analysis afforded flexibility in prospecting for suitable areas for the installation of wood panel industries in the Espírito Santo State can bring great economic and social benefits to the local population with the generation of jobs, income, tax revenues and GDP increase for the State and municipalities involved. The proposed methodology can be adapted to other areas and agricultural crops. Copyright © 2017 Elsevier Ltd. All rights reserved.

Structural elucidation of Eucalyptus lignin and its dynamic changes in the cell walls during an integrated process of ionic liquids and successive alkali treatments.

PubMed

Li, Han-Yin; Wang, Chen-Zhou; Chen, Xue; Cao, Xue-Fei; Sun, Shao-Ni; Sun, Run-Cang

2016-12-01

An integrated process based on ionic liquids ([Bmim]Cl and [Bmim]OAc) pretreatment and successive alkali post-treatments (0.5, 2.0, and 4.0% NaOH at 90°C for 2h) was performed to isolate lignins from Eucalyptus. The structural features and spatial distribution of lignin in the Eucalyptus cell wall were investigated thoroughly. Results revealed that the ionic liquids pretreatment promoted the isolation of alkaline lignin from the pretreated samples without obvious structural changes. Additionally, the integrated process resulted in syringyl-rich lignin macromolecules with more β-O-4' linkages and less phenolic hydroxyl groups. Confocal Raman microscopy analysis showed that the dissolution behavior of lignin was varied in the morphologically distinct regions during the successive alkali treatments, and lignin dissolved was mainly stemmed from the secondary wall regions. These results provided some useful information for understanding the mechanisms of delignification during the integrated process and enhancing the potential utilizations of lignin in future biorefineries. Copyright © 2016 Elsevier Ltd. All rights reserved.

Eucalyptus grandis Hill ex Maiden inoculated with Pisolithus microcarpus (UFSC-Pt116) in land subject to the sandy process in Southern Brazil

PubMed Central

de Souza, Eduardo Lorensi; Antoniolli, Zaida Inês; Machado, Rafael Goulart; Eckhardt, Daniel Pazzini; Dahmer, Sabrina de Fátima Barbosa

2014-01-01

Eucalypts is one of the main species used for commercial reforestation in the Rio Grande do Sul State, Brazil. This study aimed to evaluate the survival and early growth of eucalyptus trees in an area subject to sandy process after three years of growth. The Eucalyptus grandis seedlings were grown in a greenhouse, inoculated or not with the isolated ectomycorrhizal Pisolithus microcarpus (UFSC-Pt116), produced in peat or Entisol. After 120 days, the seedlings were transplanted to an area subject to the sandy process, in the São Francisco de Assis city, RS. The plants have been evaluated regarding survival, height, stem diameter, nitrogen, phosphorus and potassium levels and total phosphorus, inorganic phosphorus, organic phosphorus and wood production on different days after planting. The seedlings grown on the Entisol which was inoculated with the isolated UFSC-Pt116 presented higher survival rates, height, stem diameter, nitrogen concentration and wood production then non-inoculated seedlings. Inoculation with ectomycorrhizal fungi enhanced the production of E. grandis seedlings in survival rates, height, stem diameter. PMID:25763017

Supercritical Fluid Extraction of Eucalyptus globulus Bark—A Promising Approach for Triterpenoid Production

PubMed Central

Domingues, Rui M. A.; Oliveira, Eduardo L. G.; Freire, Carmen S. R.; Couto, Ricardo M.; Simões, Pedro C.; Neto, Carlos P.; Silvestre, Armando J. D.; Silva, Carlos M.

2012-01-01

Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold. PMID:22837719

Antimicrobial Susceptibility of Escherichia coli Strains Isolated from Alouatta spp. Feces to Essential Oils

PubMed Central

Carregaro, Adriano Bonfim; Santurio, Deise Flores; de Sá, Mariangela Facco; Santurio, Janio Moraes; Alves, Sydney Hartz

2016-01-01

This study evaluated the in vitro antibacterial activity of essential oils from Lippia graveolens (Mexican oregano), Origanum vulgaris (oregano), Thymus vulgaris (thyme), Rosmarinus officinalis (rosemary), Cymbopogon nardus (citronella), Cymbopogon citratus (lemongrass), and Eucalyptus citriodora (eucalyptus) against Escherichia coli (n = 22) strains isolated from Alouatta spp. feces. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for each isolate using the broth microdilution technique. Essential oils of Mexican oregano (MIC mean = 1818 μg mL−1; MBC mean = 2618 μg mL−1), thyme (MIC mean = 2618 μg mL−1; MBC mean = 2909 μg mL−1), and oregano (MIC mean = 3418 μg mL−1; MBC mean = 4800 μg mL−1) showed the best antibacterial activity, while essential oils of eucalyptus, rosemary, citronella, and lemongrass displayed no antibacterial activity at concentrations greater than or equal to 6400 μg mL−1. Our results confirm the antimicrobial potential of some essential oils, which deserve further research. PMID:27313638

The challenge of tree height in Eucalyptus regnans: when xylem tapering overcomes hydraulic resistance.

PubMed

Petit, Giai; Pfautsch, Sebastian; Anfodillo, Tommaso; Adams, Mark A

2010-09-01

*Recent research suggests that increasing conduit tapering progressively reduces hydraulic constraints caused by tree height. Here, we tested this hypothesis using the tallest hardwood species, Eucalyptus regnans. *Vertical profiles of conduit dimensions and vessel density were measured for three mature trees of height 47, 51 and 63 m. *Mean hydraulic diameter (Dh) increased rapidly from the tree apex to the point of crown insertion, with the greatest degree of tapering yet reported (b > 0.33). Conduit tapering was such that most of the total resistance was found close to the apex (82-93% within the first 1 m of stem) and the path length effect was reduced by a factor of 2000. Vessel density (VD) declined from the apex to the base of each tree, with scaling parameters being similar for all trees (a = 4.6; b = -0.5). *Eucalyptus regnans has evolved a novel xylem design that ensures a high hydraulic efficiency. This feature enables the species to grow quickly to heights of 50-60 m, beyond the maximum height of most other hardwood trees.

Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.

PubMed

Li, Han-Yin; Sun, Shao-Ni; Zhou, Xia; Peng, Feng; Sun, Run-Cang

2015-06-05

Eucalyptus was sequentially extracted with 70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C. The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies. Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy. Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit. Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall. The information obtained from the study conducted by combining chemical characterization with ultrastructure provides important basis for studying the mechanism of the alkali treatment. Copyright © 2015 Elsevier Ltd. All rights reserved.

Permanent genetic resources added to Molecular Ecology Resources Database 1 August 2011-30 September 2011.

PubMed

A'Hara, S W; Amouroux, P; Argo, Emily E; Avand-Faghih, A; Barat, Ashoktaru; Barbieri, Luiz; Bert, Theresa M; Blatrix, R; Blin, Aurélie; Bouktila, D; Broome, A; Burban, C; Capdevielle-Dulac, C; Casse, N; Chandra, Suresh; Cho, Kyung Jin; Cottrell, J E; Crawford, Charles R; Davis, Michelle C; Delatte, H; Desneux, Nicolas; Djieto-Lordon, C; Dubois, M P; El-Mergawy, R A A M; Gallardo-Escárate, C; Garcia, M; Gardiner, Mary M; Guillemaud, Thomas; Haye, P A; Hellemans, B; Hinrichsen, P; Jeon, Ji Hyun; Kerdelhué, C; Kharrat, I; Kim, Ki Hwan; Kim, Yong Yul; Kwan, Ye-Seul; Labbe, Ellen M; LaHood, Eric; Lee, Kyung Mi; Lee, Wan-Ok; Lee, Yat-Hung; Legoff, Isabelle; Li, H; Lin, Chung-Ping; Liu, S S; Liu, Y G; Long, D; Maes, G E; Magnoux, E; Mahanta, Prabin Chandra; Makni, H; Makni, M; Malausa, Thibaut; Matura, Rakesh; McKey, D; McMillen-Jackson, Anne L; Méndez, M A; Mezghani-Khemakhem, M; Michel, Andy P; Paul, Moran; Muriel-Cunha, Janice; Nibouche, S; Normand, F; Palkovacs, Eric P; Pande, Veena; Parmentier, K; Peccoud, J; Piatscheck, F; Puchulutegui, Cecilia; Ramos, R; Ravest, G; Richner, Heinz; Robbens, J; Rochat, D; Rousselet, J; Saladin, Verena; Sauve, M; Schlei, Ora; Schultz, Thomas F; Scobie, A R; Segovia, N I; Seyoum, Seifu; Silvain, J-F; Tabone, Elisabeth; Van Houdt, J K J; Vandamme, S G; Volckaert, F A M; Wenburg, John; Willis, Theodore V; Won, Yong-Jin; Ye, N H; Zhang, W; Zhang, Y X

2012-01-01

This article documents the addition of 299 microsatellite marker loci and nine pairs of single-nucleotide polymorphism (SNP) EPIC primers to the Molecular Ecology Resources (MER) Database. Loci were developed for the following species: Alosa pseudoharengus, Alosa aestivalis, Aphis spiraecola, Argopecten purpuratus, Coreoleuciscus splendidus, Garra gotyla, Hippodamia convergens, Linnaea borealis, Menippe mercenaria, Menippe adina, Parus major, Pinus densiflora, Portunus trituberculatus, Procontarinia mangiferae, Rhynchophorus ferrugineus, Schizothorax richardsonii, Scophthalmus rhombus, Tetraponera aethiops, Thaumetopoea pityocampa, Tuta absoluta and Ugni molinae. These loci were cross-tested on the following species: Barilius bendelisis, Chiromantes haematocheir, Eriocheir sinensis, Eucalyptus camaldulensis, Eucalyptus cladocalix, Eucalyptus globulus, Garra litaninsis vishwanath, Garra para lissorhynchus, Guindilla trinervis, Hemigrapsus sanguineus, Luma chequen. Guayaba, Myrceugenia colchagüensis, Myrceugenia correifolia, Myrceugenia exsucca, Parasesarma plicatum, Parus major, Portunus pelagicus, Psidium guayaba, Schizothorax richardsonii, Scophthalmus maximus, Tetraponera latifrons, Thaumetopoea bonjeani, Thaumetopoea ispartensis, Thaumetopoea libanotica, Thaumetopoea pinivora, Thaumetopoea pityocampa ena clade, Thaumetopoea solitaria, Thaumetopoea wilkinsoni and Tor putitora. This article also documents the addition of nine EPIC primer pairs for Euphaea decorata, Euphaea formosa, Euphaea ornata and Euphaea yayeyamana. © 2011 Blackwell Publishing Ltd.

Effects of steaming treatment on crystallinity and glass transition temperature of Eucalyptuses grandis × E. urophylla

NASA Astrophysics Data System (ADS)

Kong, Lulu; Zhao, Zijian; He, Zhengbin; Yi, Songlin

To investigate the effects of steaming treatment on crystallinity and glass transition temperature, samples of Eucalyptuses grandis × E. urophylla with moisture content of 50%, 70%, and 90% were steamed in saturated steam at 100 °C for 2, 4, 6, and 8 h. The degree of crystallinity (CrI) and glass transition temperature (Tg) were measured via X-ray diffraction and dynamic mechanical analysis, respectively. Results revealed a crystallinity degree of Eucalyptus of 29.9%-34.2%, and a glass transition temperature of 80-94 °C with moisture contents of steamed samples of 20%. Furthermore, steaming was revealed to have an obvious effect on crystallization and glass transition. Values of CrI and Tg showed similar changing characteristics: increasing initially, followed by a decrease with increasing steaming time, reaching a maximum at 2 h. Water within the wood seemed to promote crystallization and glass transition during steaming. All steamed samples tested in this study reached glass transition temperature after 50 min of steaming, and the residual growth stress was released.

Climate Modelling Shows Increased Risk to Eucalyptus sideroxylon on the Eastern Coast of Australia Compared to Eucalyptus albens

PubMed Central

Shabani, Farzin; Ahmadi, Mohsen

2017-01-01

Aim: To identify the extent and direction of range shift of Eucalyptus sideroxylon and E. albens in Australia by 2050 through an ensemble forecast of four species distribution models (SDMs). Each was generated using four global climate models (GCMs), under two representative concentration pathways (RCPs). Location: Australia. Methods: We used four SDMs of (i) generalized linear model, (ii) MaxEnt, (iii) random forest, and (iv) boosted regression tree to construct SDMs for species E. sideroxylon and E. albens under four GCMs including (a) MRI-CGCM3, (b) MIROC5, (c) HadGEM2-AO and (d) CCSM4, under two RCPs of 4.5 and 6.0. Here, the true skill statistic (TSS) index was used to assess the accuracy of each SDM. Results: Results showed that E. albens and E. sideroxylon will lose large areas of their current suitable range by 2050 and E. sideroxylon is projected to gain in eastern and southeastern Australia. Some areas were also projected to remain suitable for each species between now and 2050. Our modelling showed that E. sideroxylon will lose suitable habitat on the western side and will not gain any on the eastern side because this region is one the most heavily populated areas in the country, and the populated areas are moving westward. The predicted decrease in E. sideroxylon’s distribution suggests that land managers should monitor its population closely, and evaluate whether it meets criteria for a protected legal status. Main conclusions: Both Eucalyptus sideroxylon and E. albens will be negatively affected by climate change and it is projected that E. sideroxylon will be at greater risk of losing habitat than E. albens. PMID:29186755

Intraspecific variation in the growth and survival of juvenile fish exposed to Eucalyptus leachate

PubMed Central

Morrongiello, John R; Bond, Nicholas R; Crook, David A; Wong, Bob B M

2013-01-01

Whilst changes in freshwater assemblages along gradients of environmental stress have been relatively well studied, we know far less about intraspecific variation to these same stressors. A stressor common in fresh waters worldwide is leachates from terrestrial plants. Leachates alter the physiochemical environment of fresh waters by lowering pH and dissolved oxygen and also releasing toxic compounds such as polyphenols and tannins, all of which can be detrimental to aquatic organisms. We investigated how chronic exposure to Eucalyptus leaf leachate affected the growth and survival of juvenile southern pygmy perch (Nannoperca australis) collected from three populations with different litter inputs, hydrology and observed leachate concentrations. Chronic exposure to elevated leachate levels negatively impacted growth and survival, but the magnitude of these lethal and sublethal responses was conditional on body size and source population. Bigger fish had increased survival at high leachate levels but overall slower growth rates. Body size also varied among populations and fish from the population exposed to the lowest natural leachate concentrations had the highest average stress tolerance. Significant intraspecific variation in both growth and survival caused by Eucalyptus leachate exposure indicates that the magnitude (but not direction) of these stress responses varies across the landscape. This raises the potential for leachate-induced selection to operate at an among-population scale. The importance of body size demonstrates that the timing of leachate exposure during ontogeny is central in determining the magnitude of biological response, with early life stages being most vulnerable. Overall, we demonstrate that Eucalyptus leachates are prevalent and potent selective agents that can trigger important sublethal impacts, beyond those associated with more familiar fish kills, and reiterate that dissolved organic carbon is more than just an energy source in aquatic environments. PMID:24198944

Downregulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) or cinnamate-4-hydrolylase (C4H) in Eucalyptus urophylla x Eucalyptus grandis leads to increased extractability

DOE PAGES

Ziebell, Angela; Gjersing, Erica; Hinchee, Maud; ...

2016-01-20

Lignin reduction through breeding and genetic modification has the potential to reduce costs in biomass processing in pulp and paper, forage, and lignocellulosic ethanol industries. Here, we present detailed characterization of the extractability and lignin structure of Eucalyptus urophylla x Eucalyptus grandis RNAi downregulated in p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) or cinnamate-4-hydroxylase (C4H). Both the C3'H and C4H downregulated lines were found to have significantly higher extractability when exposed to NaOH base extraction, indicating altered cell wall construction. The molecular weight of isolated lignin was measured and lignin structure was determined by HSQC NMR-based lignin subunit analysis for control and themore » C3'H and C4H downregulated lines. The slight reductions in average molecular weights of the lignin isolated from the transgenic lines (C3'H = 7000, C4H = 6500, control = 7300) does not appear to explain the difference in extractability. The HSQC NMR-based lignin subunit analysis showed increases in H lignin content for the C3'H but only slight differences in the lignin subunit structure of the C3'H and C4H downregulated lines when compared to the control. The greatest difference between the C3'H and C4H downregulated lines is the total lignin content; therefore, it appears that overall lowered lignin content contributes greatly to reduced recalcitrance and increased extractability of cell wall biopolymers. Furthermore, studies will be conducted to determine how the reduction in lignin content creates a less rigid cell wall that is more prone to extraction and sugar release.« less

Ethyl Formate: A Potential Disinfestation Treatment for Eucalyptus Weevil (Gonipterus platensis) (Coleoptera: Curculionidae) in Apples.

PubMed

Agarwal, Manjree; Ren, Yonglin; Newman, James; Learmonth, Stewart

2015-12-01

Export of Pink Lady apples from Australia has been significantly affected by infestations of adult eucalyptus weevils (Gonipterus platensis Marelli). These weevils cling tenaciously to the pedicel of apple fruit when selecting overwintering sites. As a result, apples infested with live G. platensis adults lead to rejection for export. Since the Montreal Protocol restricted use of methyl bromide as postharvest treatment, it was necessary to consider alternative safer fumigants for disinfestation of eucalyptus weevil. Laboratory experiments were conducted using concentrations of 5, 10, 15, 20, 25, 30, 40, and 80 mg/liter of ethyl formate. Complete control (100% mortality) was achieved at 25-30 mg/liter of ethyl formate at 22-24°C for 24-h exposure without apples. However, with 90-95% of the volume full of apples, complete control was achieved at 40 mg/liter of ethyl formate at 22-24°C for 24-h exposure. No phytotoxicity was observed and after one day aeration, residue of ethyl formate declined to natural levels (0.05-0.2 mg/kg). Five ethyl formate field trials were conducted in cool storages (capacity from 250-900 tons) and 100% kill of eucalyptus weevils were achieved at 50-55 mg/liter at 7-10°C for 24 h. Ethyl formate has great potential for preshipment treatment of apples. Its use is considerably cheaper and safer than already existing fumigants like methyl bromide and phosphine. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Beyond habitat structure: Landscape heterogeneity explains the monito del monte (Dromiciops gliroides) occurrence and behavior at habitats dominated by exotic trees.

PubMed

Salazar, Daniela A; Fontúrbel, Francisco E

2016-09-01

Habitat structure determines species occurrence and behavior. However, human activities are altering natural habitat structure, potentially hampering native species due to the loss of nesting cavities, shelter or movement pathways. The South American temperate rainforest is experiencing an accelerated loss and degradation, compromising the persistence of many native species, and particularly of the monito del monte (Dromiciops gliroides Thomas, 1894), an arboreal marsupial that plays a key role as seed disperser. Aiming to compare 2 contrasting habitats (a native forest and a transformed habitat composed of abandoned Eucalyptus plantations and native understory vegetation), we assessed D. gliroides' occurrence using camera traps and measured several structural features (e.g. shrub and bamboo cover, deadwood presence, moss abundance) at 100 camera locations. Complementarily, we used radio telemetry to assess its spatial ecology, aiming to depict a more complete scenario. Moss abundance was the only significant variable explaining D. gliroides occurrence between habitats, and no structural variable explained its occurrence at the transformed habitat. There were no differences in home range, core area or inter-individual overlapping. In the transformed habitats, tracked individuals used native and Eucalyptus-associated vegetation types according to their abundance. Diurnal locations (and, hence, nesting sites) were located exclusively in native vegetation. The landscape heterogeneity resulting from the vicinity of native and Eucalyptus-associated vegetation likely explains D. gliroides occurrence better than the habitat structure itself, as it may be use Eucalyptus-associated vegetation for feeding purposes but depend on native vegetation for nesting. © 2016 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.

Patterns of Reproductive Isolation in Eucalyptus-A Phylogenetic Perspective.

PubMed

Larcombe, Matthew J; Holland, Barbara; Steane, Dorothy A; Jones, Rebecca C; Nicolle, Dean; Vaillancourt, René E; Potts, Brad M

2015-07-01

We assess phylogenetic patterns of hybridization in the speciose, ecologically and economically important genus Eucalyptus, in order to better understand the evolution of reproductive isolation. Eucalyptus globulus pollen was applied to 99 eucalypt species, mainly from the large commercially important subgenus, Symphyomyrtus. In the 64 species that produce seeds, hybrid compatibility was assessed at two stages, hybrid-production (at approximately 1 month) and hybrid-survival (at 9 months), and compared with phylogenies based on 8,350 genome-wide DArT (diversity arrays technology) markers. Model fitting was used to assess the relationship between compatibility and genetic distance, and whether or not the strength of incompatibility "snowballs" with divergence. There was a decline in compatibility with increasing genetic distance between species. Hybridization was common within two closely related clades (one including E. globulus), but rare between E. globulus and species in two phylogenetically distant clades. Of three alternative models tested (linear, slowdown, and snowball), we found consistent support for a snowball model, indicating that the strength of incompatibility accelerates relative to genetic distance. Although we can only speculate about the genetic basis of this pattern, it is consistent with a Dobzhansky-Muller-model prediction that incompatibilities should snowball with divergence due to negative epistasis. Different rates of compatibility decline in the hybrid-production and hybrid-survival measures suggest that early-acting postmating barriers developed first and are stronger than later-acting barriers. We estimated that complete reproductive isolation can take up to 21-31 My in Eucalyptus. Practical implications for hybrid eucalypt breeding and genetic risk assessment in Australia are discussed. © The Author 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Stand-volume estimation from multi-source data for coppiced and high forest Eucalyptus spp. silvicultural systems in KwaZulu-Natal, South Africa

NASA Astrophysics Data System (ADS)

Dube, Timothy; Sibanda, Mbulisi; Shoko, Cletah; Mutanga, Onisimo

2017-10-01

Forest stand volume is one of the crucial stand parameters, which influences the ability of these forests to provide ecosystem goods and services. This study thus aimed at examining the potential of integrating multispectral SPOT 5 image, with ancillary data (forest age and rainfall metrics) in estimating stand volume between coppiced and planted Eucalyptus spp. in KwaZulu-Natal, South Africa. To achieve this objective, Partial Least Squares Regression (PLSR) algorithm was used. The PLSR algorithm was implemented by applying three tier analysis stages: stage I: using ancillary data as an independent dataset, stage II: SPOT 5 spectral bands as an independent dataset and stage III: combined SPOT 5 spectral bands and ancillary data. The results of the study showed that the use of an independent ancillary dataset better explained the volume of Eucalyptus spp. growing from coppices (adjusted R2 (R2Adj) = 0.54, RMSEP = 44.08 m3/ha), when compared with those that were planted (R2Adj = 0.43, RMSEP = 53.29 m3/ha). Similar results were also observed when SPOT 5 spectral bands were applied as an independent dataset, whereas improved volume estimates were produced when using combined dataset. For instance, planted Eucalyptus spp. were better predicted adjusted R2 (R2Adj) = 0.77, adjusted R2Adj = 0.59, RMSEP = 36.02 m3/ha) when compared with those that grow from coppices (R2 = 0.76, R2Adj = 0.46, RMSEP = 40.63 m3/ha). Overall, the findings of this study demonstrated the relevance of multi-source data in ecosystems modelling.

Eucalyptus hairy roots, a fast, efficient and versatile tool to explore function and expression of genes involved in wood formation.

PubMed

Plasencia, Anna; Soler, Marçal; Dupas, Annabelle; Ladouce, Nathalie; Silva-Martins, Guilherme; Martinez, Yves; Lapierre, Catherine; Franche, Claudine; Truchet, Isabelle; Grima-Pettenati, Jacqueline

2016-06-01

Eucalyptus are of tremendous economic importance being the most planted hardwoods worldwide for pulp and paper, timber and bioenergy. The recent release of the Eucalyptus grandis genome sequence pointed out many new candidate genes potentially involved in secondary growth, wood formation or lineage-specific biosynthetic pathways. Their functional characterization is, however, hindered by the tedious, time-consuming and inefficient transformation systems available hitherto for eucalypts. To overcome this limitation, we developed a fast, reliable and efficient protocol to obtain and easily detect co-transformed E. grandis hairy roots using fluorescent markers, with an average efficiency of 62%. We set up conditions both to cultivate excised roots in vitro and to harden composite plants and verified that hairy root morphology and vascular system anatomy were similar to wild-type ones. We further demonstrated that co-transformed hairy roots are suitable for medium-throughput functional studies enabling, for instance, protein subcellular localization, gene expression patterns through RT-qPCR and promoter expression, as well as the modulation of endogenous gene expression. Down-regulation of the Eucalyptus cinnamoyl-CoA reductase1 (EgCCR1) gene, encoding a key enzyme in lignin biosynthesis, led to transgenic roots with reduced lignin levels and thinner cell walls. This gene was used as a proof of concept to demonstrate that the function of genes involved in secondary cell wall biosynthesis and wood formation can be elucidated in transgenic hairy roots using histochemical, transcriptomic and biochemical approaches. The method described here is timely because it will accelerate gene mining of the genome for both basic research and industry purposes. © 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

Defense responses in plants of Eucalyptus elicited by Streptomyces and challenged with Botrytis cinerea.

PubMed

Salla, Tamiris D; Astarita, Leandro V; Santarém, Eliane R

2016-04-01

Elicitation of E. grandis plants with Streptomyces PM9 reduced the gray-mold disease, through increasing the levels of enzymes directly related to the induction of plant defense responses, and accumulation of specific phenolic compounds. Members of Eucalyptus are economically important woody species, especially as a raw material in many industrial sectors. Species of this genus are susceptible to pathogens such as Botrytis cinerea (gray mold). Biological control of plant diseases using rhizobacteria is one alternative to reduce the use of pesticides and pathogen attack. This study evaluated the metabolic and phenotypic responses of Eucalyptus grandis and E. globulus plants treated with Streptomyces sp. PM9 and challenged with the pathogenic fungus B. cinerea. Metabolic responses were evaluated by assessing the activities of the enzymes polyphenol oxidase and peroxidase as well as the levels of phenolic compounds and flavonoids. The incidence and progression of the fungal disease in PM9-treated plants and challenged with B. cinerea were evaluated. Treatment with Streptomyces sp. PM9 and challenge with B. cinerea led to changes in the activities of polyphenol oxidase and peroxidase as well as in the levels of phenolic compounds in the plants at different time points. Alterations in enzymes of PM9-treated plants were related to early defense responses in E. grandis. Gallic and chlorogenic acids were on average more abundant, although caffeic acid, benzoic acid and catechin were induced at specific time points during the culture period. Treatment with Streptomyces sp. PM9 significantly delayed the establishment of gray mold in E. grandis plants. These results demonstrate the action of Streptomyces sp. PM9 in inducing plant responses against B. cinerea, making this organism a potential candidate for biological control in Eucalyptus.

UPLC-PDA-ESI-qTOF-MS profiling and potent anti-HSV-II activity of Eucalyptus sideroxylon leaves.

PubMed

Okba, Mona M; El Gedaily, Rania A; Ashour, Rehab M

2017-11-15

Eucalyptus is one of the most important and highly exploited genus in family Myrtaceae. An UPLC/PDA/ESI-qTOF-MS method was adopted to identify Eucalyptus sideroxylon Cunn. ex Woolls leaves phytoconstituents. Cytotoxicity of E. sideroxylon leaves phloroglucinol-rich extract (PGRE) on VERO cells was determined. The antiviral effect of PGRE against hepatitis A (HAV), herpes simplex type 1 (HSV-I), herpes simplex type 2 (HSV-II), coxsackie (CoxB4), and adenoviruses was in vitro evaluated using MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide). UPLC-MS analysis allowed the identification of 70 metabolites including: 26 triterpenes, 13 phloroglucinols, 8 fatty acids, 5 flavonoids, 5 oleuropeic acid glucosides, 3 gallic acid derivatives, and 10 miscellaneous. Twenty four metabolites identified in the leaves of E. sideroxylon and four in the genus Eucalyptus are reported herein for the first time. PGRE was found to be non-cytotoxic; the concentration that reduced the cell viability by 50% (CC 50 ) was 0.808mg/mL. Maximum non-toxic concentration (MNTC) of PGRE on Vero cells was 0.312mg/mL. The best antiviral activity was observed against HSV-II. Its mechanism was through decreasing the viral replication (IC 50 189.36μg/mL, 87.65% inhibition) and attachment on Vero cells (IC 50 199.34μg/mL, 83.13% inhibition) rather than virucidal effect (IC 50 293.1μg/mL, 50.68% inhibition). This study provides a complete map for E. sideroxylon leaves composition. It also suggests the plant as a source of new antiviral agents. Copyright © 2017 Elsevier B.V. All rights reserved.

Seasonal Variation of Carbon Metabolism in the Cambial Zone of Eucalyptus grandis

PubMed Central

Budzinski, Ilara G. F.; Moon, David H.; Lindén, Pernilla; Moritz, Thomas; Labate, Carlos A.

2016-01-01

Eucalyptus species are the most widely hardwood planted in the world. It is one of the successful examples of commercial forestry plantation in Brazil and other tropical and subtropical countries. The tree is valued for its rapid growth, adaptability and wood quality. Wood formation is the result of cumulative annual activity of the vascular cambium. This cambial activity is generally related to the alternation of cold and warm, and/or dry and rainy seasons. Efforts have focused on analysis of cambial zone in response to seasonal variations in trees from temperate zones. However, little is known about the molecular changes triggered by seasonal variations in trees from tropical countries. In this work we attempted to establish a global view of seasonal alterations in the cambial zone of Eucalyptus grandis Hill ex Maiden, emphasizing changes occurring in the carbon metabolism. Using transcripts, proteomics and metabolomics we analyzed the tissues harvested in summer-wet and winter-dry seasons. Based on proteomics analysis, 70 proteins that changed in abundance were successfully identified. Transcripts for some of these proteins were analyzed and similar expression patterns were observed. We identified 19 metabolites differentially abundant. Our results suggest a differential reconfiguration of carbon partioning in E. grandis cambial zone. During summer, pyruvate is primarily metabolized via ethanolic fermentation, possibly to regenerate NAD+ for glycolytic ATP production and cellular maintenance. However, in winter there seems to be a metabolic change and we found that some sugars were highly abundant. Our results revealed a dynamic change in E. grandis cambial zone due to seasonality and highlight the importance of glycolysis and ethanolic fermentation for energy generation and maintenance in Eucalyptus, a fast growing tree. PMID:27446160

Phytoremediation potential of cadmium-contaminated soil by Eucalyptus globulus under different coppice systems.

PubMed

Luo, Jie; Qi, Shihua; Peng, Li; Xie, Xianming

2015-03-01

The objective of this research was to determine the phytoremediation potential of Eucalyptus globulus in Cd contaminated soil through two different harvest methods. Although replanting is more expensive than coppicing and produces less aboveground biomass, more Cd can be removed from the soil with roots removal at each harvest as the E. globulus absorbs vast majority of heavy metals in non-metabolically active parts like roots. Despite the higher cost of replanting in a single harvest, when phytoremediation efficiency and total duration are considered as important factors, the replanting treatment should be recommended as an appropriate method which can decrease the phytoremediation time obviously.

Eucalyptus kraft black liquor enhances growth and productivity of Spirulina in outdoor cultures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chauhan, V.S.; Singh, G.; Ramamurthy, V.

1995-07-01

Mass cultivation of microalgae for commercial applications suffers from poor productivities when measured against laboratory results or theoretical projections. In an effort to reduce this gap it was discovered that addition of eucalyptus kraft black liquor (BL) enhanced biomass productivity in outdoor cultures of Spirulina by increasing growth rate by 38% and biomass yield by 43%. BL treatment resulted in elevation of nitrogen assimilating enzyme activities and efficiency of phosphate utilization. Analyses of forenoon and afternoon oxygen production rates (OPRs) indicated higher photosynthetic and respiratory activity in BL-treated cultures compared to untreated cultures. 20 refs., 1 fig., 2 tabs.

Chimpanzees (Pan troglodytes) Flexibly Use Introduced Species for Nesting and Bark Feeding in a Human-Dominated Habitat.

PubMed

McCarthy, Maureen S; Lester, Jack D; Stanford, Craig B

2017-01-01

As habitat loss and fragmentation place growing pressure on endangered nonhuman primate populations, researchers find increasing evidence for novel responses in behavior. In western Uganda between the Budongo and Bugoma Forests, chimpanzees ( Pan troglodytes schweinfurthii ) inhabit a mosaic landscape comprising forest fragments, human settlements, and agricultural land. We recorded nests and feeding evidence of unhabituated chimpanzees in this region over a 12-mo period. We found extensive evidence of nesting in introduced tree species, including eucalyptus ( Eucalyptus grandis ), guava ( Psidium guajava ), cocoa ( Theobroma cacao ), and Caribbean pine ( Pinus caribaea ). In addition, we found instances of ground nesting, nest reuse, and composite nests constructed from branches of multiple trees. This evidence may indicate a lack of suitable nesting trees or attempts by chimpanzees to nest in areas of riparian forest that allow them to avoid human detection. We also found new evidence for eucalyptus bark feeding by chimpanzees. Such evidence suggests chimpanzees respond flexibly to mitigate anthropogenic pressures in human-dominated landscapes. The limits of such flexibility remain unknown. Further research is needed to examine systematically the factors influencing the use of such resources and to understand better the extent to which chimpanzees can persist while relying on them.

Biological denitrification in marine aquaculture systems: A multiple electron donor microcosm study.

PubMed

He, Qiaochong; Zhang, Dongqing; Main, Kevan; Feng, Chuanping; Ergas, Sarina J

2018-05-08

There is a lack of information on denitrification of saline wastewaters, such as those from marine recirculating aquaculture systems (RAS), ion exchange brines and wastewater in areas where sea water is used for toilet flushing. In this study, side-by-side microcosms were used to compare methanol, fish waste (FW), wood chips, elemental sulfur (S 0 ) and a combination of wood chips and sulfur for saline wastewater denitrification. The highest denitrification rate was obtained with methanol (23.4 g N/(m 3 ·d)), followed by FW (4.5 g N/(m 3 ·d)), S 0 (3.5 g N/(m 3 ·d)), eucalyptus mulch (2.6 g N/(m 3 ·d)), and eucalyptus mulch with sulfur (2.2 g N/(m 3 ·d)). Significant differences were observed in denitrification rate for different wood species (pine > oak ≫ eucalyptus) due to differences in readily biodegradable organic carbon released. A pine wood-sulfur heterotrophic-autotrophic denitrification (P-WSHAD) process provided a high denitrification rate (7.2-11.9 g N/(m 3 ·d)), with lower alkalinity consumption and sulfate generation than sulfur alone. Copyright © 2018 Elsevier Ltd. All rights reserved.

Use of geostatistics to determine the spatial distribution and infestation rate of leaf-cutting ant nests (Hymenoptera: Formicidae) in eucalyptus plantations.

PubMed

Lasmar, O; Zanetti, R; dos Santos, A; Fernandes, B V

2012-08-01

One of the fundamental steps in pest sampling is the assessment of the population distribution in the field. Several studies have investigated the distribution and appropriate sampling methods for leaf-cutting ants; however, more reliable methods are still required, such as those that use geostatistics. The objective of this study was to determine the spatial distribution and infestation rate of leaf-cutting ant nests in eucalyptus plantations by using geostatistics. The study was carried out in 2008 in two eucalyptus stands in Paraopeba, Minas Gerais, Brazil. All of the nests in the studied area were located and used for the generation of GIS maps, and the spatial pattern of distribution was determined considering the number and size of nests. Each analysis and map was made using the R statistics program and the geoR package. The nest spatial distribution in a savanna area of Minas Gerais was clustered to a certain extent. The models generated allowed the production of kriging maps of areas infested with leaf-cutting ants, where chemical intervention would be necessary, reducing the control costs, impact on humans, and the environment.

Detection of dead standing Eucalyptus camaldulensis without tree delineation for managing biodiversity in native Australian forest

NASA Astrophysics Data System (ADS)

Miltiadou, Milto; Campbell, Neil D. F.; Gonzalez Aracil, Susana; Brown, Tony; Grant, Michael G.

2018-05-01

In Australia, many birds and arboreal animals use hollows for shelters, but studies predict shortage of hollows in near future. Aged dead trees are more likely to contain hollows and therefore automated detection of them plays a substantial role in preserving biodiversity and consequently maintaining a resilient ecosystem. For this purpose full-waveform LiDAR data were acquired from a native Eucalypt forest in Southern Australia. The structure of the forest significantly varies in terms of tree density, age and height. Additionally, Eucalyptus camaldulensis have multiple trunk splits making tree delineation very challenging. For that reason, this paper investigates automated detection of dead standing Eucalyptus camaldulensis without tree delineation. It also presents the new feature of the open source software DASOS, which extracts features for 3D object detection in voxelised FW LiDAR. A random forest classifier, a weighted-distance KNN algorithm and a seed growth algorithm are used to create a 2D probabilistic field and to then predict potential positions of dead trees. It is shown that tree health assessment is possible without tree delineation but since it is a new research directions there are many improvements to be made.

Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes

PubMed Central

da Luz, José Maria Rodrigues; Nunes, Mateus Dias; Paes, Sirlaine Albino; Torres, Denise Pereira; de Cássia Soares da Silva, Marliane; Kasuya, Maria Catarina Megumi

2012-01-01

The mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). Understanding how these enzymes work will help to improve the productivity of mushroom cultures and decrease the potential pollution that can be caused by inadequate discharge of the agroindustrial residues. The objective of this work was to assess the activity of the lignocellulolytic enzymes produced by two P. ostreatus strains (PLO 2 and PLO 6). These strains were used to inoculate samples of coffee husks, eucalyptus sawdust or eucalyptus bark add with or without 20 % rice bran. Every five days after substrate inoculation, the enzyme activity and soluble protein concentration were evaluated. The maximum activity of oxidative enzymes was observed at day 10 after inoculation, and the activity of the hydrolytic enzymes increased during the entire period of the experiment. The results show that substrate composition and colonization time influenced the activity of the lignocellulolytic enzymes. PMID:24031982

New species, hyper-diversity and potential importance of Calonectria spp. from Eucalyptus in South China

PubMed Central

Lombard, L.; Chen, S.F.; Mou, X.; Zhou, X.D.; Crous, P.W.; Wingfield, M.J.

2015-01-01

Plantation forestry is expanding rapidly in China to meet an increasing demand for wood and pulp products globally. Fungal pathogens including species of Calonectria represent a serious threat to the growth and sustainability of this industry. Surveys were conducted in the Guangdong, Guangxi and Hainan Provinces of South China, where Eucalyptus trees in plantations or cuttings in nurseries displayed symptoms of leaf blight. Isolations from symptomatic leaves and soils collected close to infected trees resulted in a large collection of Calonectria isolates. These isolates were identified using the Consolidated Species Concept, employing morphological characters and DNA sequence comparisons for the β-tubulin, calmodulin, histone H3 and translation elongation factor 1-alpha gene regions. Twenty-one Calonectria species were identified of which 18 represented novel taxa. Of these, 12 novel taxa belonged to Sphaero-Naviculate Group and the remaining six to the Prolate Group. Southeast Asia appears to represent a centre of biodiversity for the Sphaero-Naviculate Group and this fact could be one of the important constraints to Eucalyptus forestry in China. The remarkable diversity of Calonectria species in a relatively small area of China and associated with a single tree species is surprising. PMID:26955194

Exploring the performance of the SEDD model to predict sediment yield in eucalyptus plantations. Long-term results from an experimental catchment in Southern Italy

NASA Astrophysics Data System (ADS)

Porto, P.; Cogliandro, V.; Callegari, G.

2018-01-01

In this paper, long-term sediment yield data, collected in a small (1.38 ha) Calabrian catchment (W2), reafforested with eucalyptus trees (Eucalyptus occidentalis Engl.) are used to validate the performance of the SEdiment Delivery Distributed Model (SEDD) in areas with high erosion rates. At first step, the SEDD model was calibrated using field data collected in previous field campaigns undertaken during the period 1978-1994. This first phase allowed the model calibration parameter β to be calculated using direct measurements of rainfall, runoff, and sediment output. The model was then validated in its calibrated form for an independent period (2006-2016) for which new measurements of rainfall, runoff and sediment output are also available. The analysis, carried out at event and annual scale showed good agreement between measured and predicted values of sediment yield and suggested that the SEDD model can be seen as an appropriate means of evaluating erosion risk associated with manmade plantations in marginal areas. Further work is however required to test the performance of the SEDD model as a prediction tool in different geomorphic contexts.

Ectomycorrhizal fungi of the Seychelles: diversity patterns and host shifts from the native Vateriopsis seychellarum (Dipterocarpaceae) and Intsia bijuga (Caesalpiniaceae) to the introduced Eucalyptus robusta (Myrtaceae), but not Pinus caribea (Pinaceae).

PubMed

Tedersoo, Leho; Suvi, Triin; Beaver, Katy; Kõljalg, Urmas

2007-01-01

Ectomycorrhizal (ECM) fungi form highly diverse communities in temperate forests, but little is known about their community ecology in tropical ecosystems. Using anatomotyping and rDNA sequencing, ECM fungi were identified on root tips of the introduced Eucalyptus robusta and Pinus caribea as well as the endemic Vateriopsis seychellarum and indigenous Intsia bijuga in the Seychelles. Sequencing revealed 30 species of ECM fungi on root tips of V. seychellarum and I. bijuga, with three species overlapping. Eucalyptus robusta shared five of these taxa, whereas P. caribea hosted three unique species of ECM fungi that were likely cointroduced with containerized seedlings. The thelephoroid (including the anamorphic genus Riessiella), euagaric, boletoid and hymenochaetoid clades of basidiomycetes dominated the ECM fungal community of native trees. Two species of Annulatascaceae (Sordariales, Ascomycota) were identified and described as ECM symbionts of V. seychellarum. The low diversity of native ECM fungi is attributed to deforestation and long-term isolation of the Seychelles. Native ECM fungi associate with exotic eucalypts, whereas cointroduced ECM fungi persist in pine plantations for decades.

Thinning regimes and initial spacing for Eucalyptus plantations in Brazil.

PubMed

Ferraz Filho, Antonio C; Mola-Yudego, Blas; González-Olabarria, José R; Scolforo, José Roberto S

2018-01-01

This study focuses on the effects of different thinning regimes on clonal Eucalyptus plantations growth. Four different trials, planted in 1999 and located in Bahia and Espírito Santo States, were used. Aside from thinning, initial planting density, and post thinning fertilization application were also evaluated. Before canopy closure, and therefore before excessive competition between trees took place, it was found that stands planted under low densities (667 trees per hectare) presented a lower mortality proportion when compared to stand planted under higher densities (1111 trees per hectare). However, diameter growth prior to thinning operations was not statistically different between these two densities, presenting an overall mean of 4.9 cm/year. After canopy closure and the application of the thinning treatments, it was found that thinning regimes beginning early in the life of the stand and leaving a low number of residual trees presented the highest diameter and height growth. Unthinned treatments and thinning regimes late in the life of the stand (after 5.5 years), leaving a large number of residual trees presented the highest values of basal area production. The choice of the best thinning regime for Eucalyptus clonal material will vary according to the plantation objective.

Activated carbon and biochar from agricultural by-products in the adorption of Cd, Pb and Zn under laboratory conditions

NASA Astrophysics Data System (ADS)

Coscione, Aline; Zini, Barbara

2015-04-01

The immobilization of inorganic contaminants by using biochar in soils has played an increasingly important role and it is seen as an attractive alternative for the remediation of heavy metals. Although, the production of activated carbon (CA) from agricultural by-products has received special attention, the activation of the the organic source has been studied in order to increase its porposity, surface area and chemical polarity, resulting in higher adsorption of metals. Therefore, this study aimed to evaluate the effectiveness of BC and CA samples, obtained from a eucalyptus husks and cane sugar bagasse after activation with 20% phosphoric acid and pyrolyzed at 450oC in the retention of Zn, Cd and Pb using contaminated individual solutions. The experiment was performed using samples of activated carbon of eucalyptus husk (CCA), eucalyptus husk biochar (BC), activated carbon of sugar cane bagasse (CBA) and sugar cane bagasse biochar (BB), treated with Zn, Cd (range of tested solution from 0.1 up to 12 mmol L-1) and Pb (from 0.1 up 50 mmol L-1) and the adjustemento of Langmuir adsorption isotherms. Samples obtained from bagasse presented higher adsoprtion of the metals tested then eucalyptus. Also the activation process had not the expected effect on either eucalyptus and bagasse samples The maxmum adsorption capacyty of samples were as follws, in mmol g-1: for Cd - 0.36 for BC; 0.32 for CCA; 0.40 for BB; 0.31 for CBA. For Zn- 0.14 for BC; no adsorbed by CCA; 0.35 5 for BB; 0.06 for CBA. For Pb - 1.24 for BC; 0.40 for CCA; 0,45 for BB; 0,03 for CBA. However, it was also observed that due to the activation with phosphoric acid, the pH of the activated carbon (CCA and CBA) were 2.4 and 2.5 in comparison with the biochars not activated (BC and BB) 9.7 and 7.0 respectively. Thus, it is yet not possible to state if the calculate capacity is due exclusively to the complexation of chemical groups in the surface of samples or to which extent there is a contribution of precipitation caused by the basic pH (non-activated) biochar samples, as shown for Zn and Pb.

Effects of pretreatment on morphology, chemical composition and enzymatic digestibility of eucalyptus bark: a potentially valuable source of fermentable sugars for biofuel production - part 1.

PubMed

Lima, Marisa A; Lavorente, Gabriela B; da Silva, Hana Kp; Bragatto, Juliano; Rezende, Camila A; Bernardinelli, Oigres D; Deazevedo, Eduardo R; Gomez, Leonardo D; McQueen-Mason, Simon J; Labate, Carlos A; Polikarpov, Igor

2013-05-09

In recent years, the growing demand for biofuels has encouraged the search for different sources of underutilized lignocellulosic feedstocks that are available in sufficient abundance to be used for sustainable biofuel production. Much attention has been focused on biomass from grass. However, large amounts of timber residues such as eucalyptus bark are available and represent a potential source for conversion to bioethanol. In the present paper, we investigate the effects of a delignification process with increasing sodium hydroxide concentrations, preceded or not by diluted acid, on the bark of two eucalyptus clones: Eucalyptus grandis (EG) and the hybrid, E. grandis x urophylla (HGU). The enzymatic digestibility and total cellulose conversion were measured, along with the effect on the composition of the solid and the liquor fractions. Barks were also assessed using Fourier-transform infrared spectroscopy (FTIR), solid-state nuclear magnetic resonance (NMR), X-Ray diffraction, and scanning electron microscopy (SEM). Compositional analysis revealed an increase in the cellulose content, reaching around 81% and 76% of glucose for HGU and EG, respectively, using a two-step treatment with HCl 1%, followed by 4% NaOH. Lignin removal was 84% (HGU) and 79% (EG), while the hemicellulose removal was 95% and 97% for HGU and EG, respectively. However, when we applied a one-step treatment, with 4% NaOH, higher hydrolysis efficiencies were found after 48 h for both clones, reaching almost 100% for HGU and 80% for EG, in spite of the lower lignin and hemicellulose removal. Total cellulose conversion increased from 5% and 7% to around 65% for HGU and 59% for EG. NMR and FTIR provided important insight into the lignin and hemicellulose removal and SEM studies shed light on the cell-wall unstructuring after pretreatment and lignin migration and precipitation on the fibers surface, which explain the different hydrolysis rates found for the clones. Our results show that the single step alkaline pretreatment improves the enzymatic digestibility of Eucalyptus bark. Furthermore, the chemical and physical methods combined in this study provide a better comprehension of the pretreatment effects on cell-wall and the factors that influence enzymatic digestibility of this forest residue.

Effects of pretreatment on morphology, chemical composition and enzymatic digestibility of eucalyptus bark: a potentially valuable source of fermentable sugars for biofuel production – part 1

PubMed Central

2013-01-01

Background In recent years, the growing demand for biofuels has encouraged the search for different sources of underutilized lignocellulosic feedstocks that are available in sufficient abundance to be used for sustainable biofuel production. Much attention has been focused on biomass from grass. However, large amounts of timber residues such as eucalyptus bark are available and represent a potential source for conversion to bioethanol. In the present paper, we investigate the effects of a delignification process with increasing sodium hydroxide concentrations, preceded or not by diluted acid, on the bark of two eucalyptus clones: Eucalyptus grandis (EG) and the hybrid, E. grandis x urophylla (HGU). The enzymatic digestibility and total cellulose conversion were measured, along with the effect on the composition of the solid and the liquor fractions. Barks were also assessed using Fourier-transform infrared spectroscopy (FTIR), solid-state nuclear magnetic resonance (NMR), X-Ray diffraction, and scanning electron microscopy (SEM). Results Compositional analysis revealed an increase in the cellulose content, reaching around 81% and 76% of glucose for HGU and EG, respectively, using a two-step treatment with HCl 1%, followed by 4% NaOH. Lignin removal was 84% (HGU) and 79% (EG), while the hemicellulose removal was 95% and 97% for HGU and EG, respectively. However, when we applied a one-step treatment, with 4% NaOH, higher hydrolysis efficiencies were found after 48 h for both clones, reaching almost 100% for HGU and 80% for EG, in spite of the lower lignin and hemicellulose removal. Total cellulose conversion increased from 5% and 7% to around 65% for HGU and 59% for EG. NMR and FTIR provided important insight into the lignin and hemicellulose removal and SEM studies shed light on the cell-wall unstructuring after pretreatment and lignin migration and precipitation on the fibers surface, which explain the different hydrolysis rates found for the clones. Conclusion Our results show that the single step alkaline pretreatment improves the enzymatic digestibility of Eucalyptus bark. Furthermore, the chemical and physical methods combined in this study provide a better comprehension of the pretreatment effects on cell-wall and the factors that influence enzymatic digestibility of this forest residue. PMID:23657132

A high-density transcript linkage map with 1,845 expressed genes positioned by microarray-based Single Feature Polymorphisms (SFP) in Eucalyptus

PubMed Central

2011-01-01

Background Technological advances are progressively increasing the application of genomics to a wider array of economically and ecologically important species. High-density maps enriched for transcribed genes facilitate the discovery of connections between genes and phenotypes. We report the construction of a high-density linkage map of expressed genes for the heterozygous genome of Eucalyptus using Single Feature Polymorphism (SFP) markers. Results SFP discovery and mapping was achieved using pseudo-testcross screening and selective mapping to simultaneously optimize linkage mapping and microarray costs. SFP genotyping was carried out by hybridizing complementary RNA prepared from 4.5 year-old trees xylem to an SFP array containing 103,000 25-mer oligonucleotide probes representing 20,726 unigenes derived from a modest size expressed sequence tags collection. An SFP-mapping microarray with 43,777 selected candidate SFP probes representing 15,698 genes was subsequently designed and used to genotype SFPs in a larger subset of the segregating population drawn by selective mapping. A total of 1,845 genes were mapped, with 884 of them ordered with high likelihood support on a framework map anchored to 180 microsatellites with average density of 1.2 cM. Using more probes per unigene increased by two-fold the likelihood of detecting segregating SFPs eventually resulting in more genes mapped. In silico validation showed that 87% of the SFPs map to the expected location on the 4.5X draft sequence of the Eucalyptus grandis genome. Conclusions The Eucalyptus 1,845 gene map is the most highly enriched map for transcriptional information for any forest tree species to date. It represents a major improvement on the number of genes previously positioned on Eucalyptus maps and provides an initial glimpse at the gene space for this global tree genome. A general protocol is proposed to build high-density transcript linkage maps in less characterized plant species by SFP genotyping with a concurrent objective of reducing microarray costs. HIgh-density gene-rich maps represent a powerful resource to assist gene discovery endeavors when used in combination with QTL and association mapping and should be especially valuable to assist the assembly of reference genome sequences soon to come for several plant and animal species. PMID:21492453

Determination of Thermal Properties and Morphology of Eucalyptus Wood Residue Filled High Density Polyethylene Composites

PubMed Central

Mengeloglu, Fatih; Kabakci, Ayse

2008-01-01

Thermal behaviors of eucalyptus wood residue (EWR) filled recycled high density polyethylene (HDPE) composites have been measured applying the thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Morphology of the materials was also studied using scanning electron microscope (SEM). Addition of the EWR into the recycled HDPE matrix reduced the starting of degradation temperature. EWR filled recycled HDPE had two main decomposition peaks, one for EWR around 350 °C and one for recycled HDPE around 460 °C. Addition of EWR did not affect the melting temperature of the recycled HDPE. Morphological study showed that addition of coupling agent improved the compatibility between wood residue and recycled HDPE. PMID:19325736

Organosolv delignification of Eucalyptus globulus: Kinetic study of autocatalyzed ethanol pulping

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oliet, M.; Rodriguez, F.; Santos, A.

2000-01-01

The autocatalyzed delignification of Eucalyptus globulus in 50% ethanol (w/w) was modeled as the irreversible and consecutive dissolution of initial, bulk, and residual lignin. Their respective contributions to total lignin was estimated as 9, 75, and 16%. Isothermal pulping experiments were carried out to evaluate an empirical kinetic model among eight proposals corresponding to different reaction schemes. The calculated activation energy was found to be 96.5, 98.5, and 40.8 kJ/mol for initial, bulk, and residual delignification, respectively. The influence of hydrogen ion concentration was expressed by a power-law function model. The kinetic model developed here was validated using data frommore » nonisothermal pulping runs.« less

Social implications of Eucalyptus propagation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prasad, G.N.N.; Ramaswamy, S.R.

1992-12-31

The role of eucalypts for planting in social forestry programs in discussed from the point of view of a non-governmental organization concerned with the welfare of rural people who depend on common and wastelands of their livelihood. Plantations carried out under Social Forestry programs have provided industrial wood rather than satisfying the needs of local people for fuel and other forest products. It would be unrealistic and futile to discuss the Eucalyptus issue in isolation. In order to appreciate the various repercussions of problems arising from plantation forestry, it is necessary to view it in a socio-economic context. The Eucalyptusmore » hybrid has been aggressively promoted by the Karnataka Forest Department under the Social Forestry Project.« less

Yeast and Macroinvertebrate Communities Associated with Leaf Litter Decomposition in a Second Order Stream

NASA Astrophysics Data System (ADS)

Sampaio, Ana; Cortes, Rui; Leão, Cecília

2004-11-01

The composition of yeast and macroinvertebrate communities was studied on black alder, blue gum eucalyptus and English oak leaves decaying in a stream during a six-month period. ANOVA analysis showed significantly different values (p < 0.0001) of yeast and macroinvertebrate densities among the three leaf litters. Some yeast species such as Cryptococcus albidus (Saito), C. laurentii (Kufferath), Rhodothorula glutinis (Fresenius), R. colostri (Castelli), and Debaryomyces hansenii (Lodder and Kreger-van Rij) were present in all litter types. Other yeasts were restricted to a specific type of litter. Macroinvertebrates were dominated by collectors-gatherers on oak and eucalyptus leaves. Shredders reached highest densities in alder leaves. (

Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador

PubMed Central

García-Tenesaca, Marilyn; Navarrete, Eillen S.; Iturralde, Gabriel A.; Villacrés Granda, Irina M.; Tejera, Eduardo; Beltrán-Ayala, Pablo

2017-01-01

Three types of monofloral honey from the Andean regions of Ecuador (Avocado, Eucalyptus, and Rapeseed honey) were analyzed to determine their floral origin, physicochemical parameters, chemical composition, antioxidant capacity, and their capacity to reduce in vitro bacterial biofilms. The chemical composition varied considerably depending on floral origin. The highest values of bioactive compounds were found in Avocado honey, classified as dark amber in color, while the lowest values were found in Eucalyptus honey followed by Rapeseed honey, both classified as extra light amber. When compared to Eucalyptus and Rapeseed honey, Avocado honey showed a more effective superoxide scavenging activity, chelating metal ions capacity, and a higher ability to protect human erythrocyte membranes against lipid peroxidation. For antimicrobial activity, the hydrogen peroxide content and the capacity to inhibit the biofilm formation, and to remove preformed biofilm from Staphylococcus aureus and Klebsiella pneumoniae was determined. Avocado honey showed the highest values of hydrogen peroxide content, as well as the highest capacity to reduce in vitro bacterial biofilms. A correlation between color vs. phenolics content vs. superoxide scavenging activity vs. chelating metal ions capacity, and the capacity to protect human erythrocyte membranes against lipid peroxidation was found. PMID:29295525

Influence of Botanical Origin and Chemical Composition on the Protective Effect against Oxidative Damage and the Capacity to Reduce In Vitro Bacterial Biofilms of Monofloral Honeys from the Andean Region of Ecuador.

PubMed

García-Tenesaca, Marilyn; Navarrete, Eillen S; Iturralde, Gabriel A; Villacrés Granda, Irina M; Tejera, Eduardo; Beltrán-Ayala, Pablo; Giampieri, Francesca; Battino, Maurizio; Alvarez-Suarez, José M

2017-12-23

Three types of monofloral honey from the Andean regions of Ecuador (Avocado, Eucalyptus, and Rapeseed honey) were analyzed to determine their floral origin, physicochemical parameters, chemical composition, antioxidant capacity, and their capacity to reduce in vitro bacterial biofilms. The chemical composition varied considerably depending on floral origin. The highest values of bioactive compounds were found in Avocado honey, classified as dark amber in color, while the lowest values were found in Eucalyptus honey followed by Rapeseed honey, both classified as extra light amber. When compared to Eucalyptus and Rapeseed honey, Avocado honey showed a more effective superoxide scavenging activity, chelating metal ions capacity, and a higher ability to protect human erythrocyte membranes against lipid peroxidation. For antimicrobial activity, the hydrogen peroxide content and the capacity to inhibit the biofilm formation, and to remove preformed biofilm from Staphylococcus aureus and Klebsiella pneumoniae was determined. Avocado honey showed the highest values of hydrogen peroxide content, as well as the highest capacity to reduce in vitro bacterial biofilms. A correlation between color vs. phenolics content vs. superoxide scavenging activity vs. chelating metal ions capacity, and the capacity to protect human erythrocyte membranes against lipid peroxidation was found.

Low doses of glyphosate enhance growth, CO2 assimilation, stomatal conductance and transpiration in sugarcane and eucalyptus.

PubMed

Nascentes, Renan F; Carbonari, Caio A; Simões, Plinio S; Brunelli, Marcela C; Velini, Edivaldo D; Duke, Stephen O

2018-05-01

Sublethal doses of herbicides can enhance plant growth and stimulate other process, an effect known as hormesis. The magnitude of hormesis is dependent on the plant species, the herbicide and its dose, plant development stage and environmental parameters. Glyphosate hormesis is well established, but relatively little is known of the mechanism of this phenomenon. The objective of this study was to determine if low doses of glyphosate that cause growth stimulation in sugarcane and eucalyptus concomitantly stimulate CO 2 assimilation. Shoot dry weight in both species increased at both 40 and 60 days after application of 6.2 to 20.2 g a.e. ha -1 glyphosate. The level of enhanced shoot dry weight was 11 to 37%, depending on the time after treatment and the species. Concomitantly, CO 2 assimilation, stomatal conductance and transpiration were increased by glyphosate doses similar to those that caused growth increases. Glyphosate applied at low doses increased the dry weight of sugarcane and eucalyptus plants in all experiments. This hormetic effect was related to low dose effects on CO 2 assimilation rate, stomatal conductance and transpiration rate, indicating that low glyphosate doses enhance photosynthesis of plants. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Genomic patterns of species diversity and divergence in Eucalyptus.

PubMed

Hudson, Corey J; Freeman, Jules S; Myburg, Alexander A; Potts, Brad M; Vaillancourt, René E

2015-06-01

We examined genome-wide patterns of DNA sequence diversity and divergence among six species of the important tree genus Eucalyptus and investigated their relationship with genomic architecture. Using c. 90 range-wide individuals of each Eucalyptus species (E. grandis, E. urophylla, E. globulus, E. nitens, E. dunnii and E. camaldulensis), genetic diversity and divergence were estimated from 2840 polymorphic diversity arrays technology markers covering the 11 chromosomes. Species differentiating markers (SDMs) identified in each of 15 pairwise species comparisons, along with species diversity (HHW ) and divergence (FST ), were projected onto the E. grandis reference genome. Across all species comparisons, SDMs totalled 1.1-5.3% of markers and were widely distributed throughout the genome. Marker divergence (FST and SDMs) and diversity differed among and within chromosomes. Patterns of diversity and divergence were broadly conserved across species and significantly associated with genomic features, including the proximity of markers to genes, the relative number of clusters of tandem duplications, and gene density within or among chromosomes. These results suggest that genomic architecture influences patterns of species diversity and divergence in the genus. This influence is evident across the six species, encompassing diverse phylogenetic lineages, geography and ecology. © 2015 University of Tasmania New Phytologist © 2015 New Phytologist Trust.

[1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina].

PubMed

Davel, G; Abrantes, R; Brudny, M; Córdoba, S; Rodero, L; Canteros, C E; Perrotta, D

2003-01-01

Two strains of C. neoformans var. gattii serotype B were isolated from a park in Buenos Aires city, Argentina. Samples were collected in spring, rubbing with swabs on the inner hollow of Eucalyptus spp. and other trees, which were not identified at the collection moment. Samples were swabbed directly onto cafeic acid with antibiotic agar, then incubated at 28 degrees C and observed daily for a week. Isolates were recovered from cafeic acid medium showing characteristic colonies of the species that allowed differentiation from other microorganisms of the sample. Identification was performed using carbohydrate fermentation, assimilation of nitrogen and carbon sources, and urease and phenoloxidase detection. Strain variety was determined with canavanine-glicine-bromotimol blue agar medium (CGB). Genotypic characterization using AP-PCR with (GACA)4 aleatory primer showed that both band profiles could be differentiated by only one band. These two strains were isolated from tree species different from Eucalyptus spp. These isolates from natural sources demonstrate that C. neoformans var. gattii efosts in Buenos Aires city environment and that it is not necessarily associated with Eucalyptus spp. The presence of C. neoformansvar. gattii in the environment, detected for the first time in Argentina, may explain its sporadic association with human infections in the local population.

Nymphal development and lerp construction of Glycaspis sp. (Hemiptera: Psylloidea) on Eucalyptus sideroxylon (Myrtaceae) in central-west New South Wales, Australia.

PubMed

Sharma, Anamika; Raman, Anantanarayanan; Taylor, Gary; Fletcher, Murray

2013-11-01

The ability to form lerps is common in Australian Psylloidea. Various species of Glycaspis Taylor (Aphalaridae) form conical lerps on different species of Eucalyptus. Lerps, being a rich sugar source, are preferentially fed by bell miners (Aves: Meliphagidae). In this paper we report the process of lerp construction by a purported new species of Glycaspis living on Eucalyptus sideroxylon. This taxon constructs lerps with anal sugary exudates first building the ribs of the lerps and later filling the space between the polymerized ribs with horizontal tractions of the same sugary substance. Developmental events in this taxon do not follow Dyar's rule strictly. Oviposition induces a non-lethal hypersensitive response in the host leaf. Population trends assessed in 2011-2012 and 2012-2013 showed distinct variations, which have been related to environmental factors. In the 2012-2013 sampling, oviposition occurred preferentially proximally to leaf cracks (possibly induced by intense frost events); we infer that this could be a strategy of this taxon to establish an 'easier' access to moisture. Sensillar designs vary between the nymphal instars and adults. The trichoid sensilla and sensillar cavities on the antennae perform mechanosensory and olfactory functions. The mouthpart complex includes a relatively long stylet bundle. Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical composition of particles from traditional burning of Pakistani wood species

NASA Astrophysics Data System (ADS)

Shahid, Imran; Kistler, Magdalena; Mukhtar, Azam; Ramirez-Santa Cruz, Carlos; Bauer, Heidi; Puxbaum, Hans

2015-11-01

Total particulate matter (TPM) emitted during burning of three types of Pakistani wood (eucalyptus camaldulensis, local name Safeeda; acacia nilotica, local name Kikar, Babul; dalbergia sissoo, Shisham, Tali) in a traditional brick stove were collected and analyzed for anhydrosugars, sugar alcohols, trace metals, soluble ions and carbonaceous species. This is a first study reporting anhydrosugars in wood smoke particles emitted during traditional burning of common wood types in Pakistan. Carbonaceous species showed the highest contribution to the particulate matter. Although the total carbon (TC) contribution was similar for all burnings (64.8-70.2%), the EC/OC ratio varied significantly, from 0.2 to 0.3 for Accacia and Dalbergia to 0.7-0.8 for Eucalyptus and Wood-mix. Among inorganic constituents potassium chloride and silicon were found at levels higher than 1%. The levoglucosan concentrations ranged from 3.0 to 6.6% (average 5.6%) with the highest value for Accacia and lowest value for the wood-mix. The high levoglucosan/mannosan ratios of 20-28 were typical for hardwood. The ratio between levoglucosan and galactosan varied stronger and was found to be around 13-20 for Accacia, Eucalyptus and Wood mix, and 43 for Dalbergia. The determined levoglucosan concentrations allowed assessing the conversion factor for calculation of biomass smoke contribution to ambient particulate matter levels in Pakistan.

New species of Mycosphaerella from Myrtaceae in plantations and native forests in eastern Australia.

PubMed

Carnegie, Angus J; Burgess, Treena I; Beilharz, Vyrna; Wingfield, Michael J

2007-01-01

The majority of Mycosphaerella species from eucalypts (Eucalyptus, Corymbia and Angophora) in Australia have been recorded only from trees growing in plantations. This illustrates a bias in research in the past two decades toward commercial enterprise, and it emphasises a lack of understanding of the occurrence of these important fungi under natural conditions. Surveys of foliar fungi in native forests in eastern Australia, as well as adjacent plantations, thus have been initiated in recent years. In this study we describe four new species of Mycosphaerella from Eucalyptus spp. as well as other Myrtaceae. Mycosphaerella tumulosa sp. nov. (anamorph: Pseudocercospora sp.) was found on more than seven species of Eucalyptus and Corymbia in native forests and plantations in northeastern New South Wales and southeastern Queensland and appears to be relatively common, although not damaging to these trees. Mycosphaerella multiseptata sp. nov. was recorded from several locations on species of Angophora in native forests and amenity plantings. Mycosphaerella pseudovespa sp. nov. was found in one location in native forest on E. biturbinata. The first species of Mycosphaerella to be described from Syncarpia, M. syncarpiae sp. nov., was found in native forests in numerous locations from Sydney through to northeastern New South Wales and appears to be relatively common.

Enrichment of arbuscular mycorrhizal fungi in a contaminated soil after rehabilitation.

PubMed

Lopes Leal, Patrícia; Varón-López, Maryeimy; Gonçalves de Oliveira Prado, Isabelle; Valentim Dos Santos, Jessé; Fonsêca Sousa Soares, Cláudio Roberto; Siqueira, José Oswaldo; de Souza Moreira, Fatima Maria

Spore counts, species composition and richness of arbuscular mycorrhizal fungi, and soil glomalin contents were evaluated in a soil contaminated with Zn, Cu, Cd and Pb after rehabilitation by partial replacement of the contaminated soil with non-contaminated soil, and by Eucalyptus camaldulensis planting with and without Brachiaria decumbens sowing. These rehabilitation procedures were compared with soils from contaminated non-rehabilitated area and non-contaminated adjacent soils. Arbuscular mycorrhizal fungi communities attributes were assessed by direct field sampling, trap culture technique, and by glomalin contents estimate. Arbuscular mycorrhizal fungi was markedly favored by rehabilitation, and a total of 15 arbuscular mycorrhizal fungi morphotypes were detected in the studied area. Species from the Glomus and Acaulospora genera were the most common mycorrhizal fungi. Number of spores was increased by as much as 300-fold, and species richness almost doubled in areas rehabilitated by planting Eucalyptus in rows and sowing B. decumbens in inter-rows. Contents of heavy metals in the soil were negatively correlated with both species richness and glomalin contents. Introduction of B. decumbens together with Eucalyptus causes enrichment of arbuscular mycorrhizal fungi species and a more balanced community of arbuscular mycorrhizal fungi spores in contaminated soil. Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.

Forest age-induced changes in evapotranspiration and water yield in a eucalypt forest

NASA Astrophysics Data System (ADS)

Cornish, P. M.; Vertessy, R. A.

2001-02-01

Water yields in a regrowth eucalypt forest were found to increase initially and then to decline below pre-treatment levels during the 16-year period which followed the logging of a moist old-growth eucalypt forest in Eastern Australia. Both regrowth and old-growth stands were dominated by Sydney Blue Gum ( Eucalyptus saligna Smith) and Silvertop Stringybark ( Eucalyptus laevopinea R. Baker). Using a paired-catchment approach we observed significant reductions in five of six gauged catchments, and were able to associate their magnitude with forest growth rate, canopy cover and soil depth. Regular yield declines were interrupted for a period in some catchments, possibly due to foliar insect attack. Yield reductions of up to a maximum 600 mm per year in logged and regenerated areas were in accord with water yield reductions observed in Mountain Ash ( Eucalyptus regnans F.J. Muell.) regeneration in Victoria. This study therefore represents the first confirmation of these Maroondah Mountain Ash results in another forest type that has also undergone eucalypt-to-eucalypt succession. Baseflow analysis indicated that baseflow and stormflow both increased after logging, with stormflow increases dominant in catchments with shallower soils. The lower runoff observed when the regenerating forest was aged 13-16 years was principally a consequence of lower baseflow.

Genetic Mapping of Quantitative Trait Loci Controlling Growth and Wood Quality Traits in Eucalyptus Grandis Using a Maternal Half-Sib Family and Rapd Markers

PubMed Central

Grattapaglia, D.; Bertolucci, FLG.; Penchel, R.; Sederoff, R. R.

1996-01-01

Quantitative trait loci (QTL) mapping of forest productivity traits was performed using an open pollinated half-sib family of Eucalyptus grandis. For volume growth, a sequential QTL mapping approach was applied using bulk segregant analysis (BSA), selective genotyping (SG) and cosegregation analysis (CSA). Despite the low heritability of this trait and the heterogeneous genetic background employed for mapping. BSA detected one putative QTL and SG two out of the three later found by CSA. The three putative QTL for volume growth were found to control 13.7% of the phenotypic variation, corresponding to an estimated 43.7% of the genetic variation. For wood specific gravity five QTL were identified controlling 24.7% of the phenotypic variation corresponding to 49% of the genetic variation. Overlapping QTL for CBH, WSG and percentage dry weight of bark were observed. A significant case of digenic epistasis was found, involving unlinked QTL for volume. Our results demonstrate the applicability of the within half-sib design for QTL mapping in forest trees and indicate the existence of major genes involved in the expression of economically important traits related to forest productivity in Eucalyptus grandis. These findings have important implications for marker-assisted tree breeding. PMID:8913761

Entomocidal activity of microwave energy & some aqueous plant extracts against Tribolium castaneum Herbst & Trogoderma granarium Everts

NASA Astrophysics Data System (ADS)

Agha, W. N. A.; Amin, A. H.; Khidr, S. K.; Ismail, A. Y.

2017-09-01

A laboratory experiment was conducted in order to evaluate the efficacy of microwave radiation and aqueous plant extracts against red flour beetle Tribolium castaneum & khapra beetle Trogoderma granarium. The larvae stage with dried fruits (black raisin, red raisin, fig and apricot) were subjected to microwave radiation at different power levels (280,560 and 840) watt for three exposure times (10, 30 and 50) seconds. Mortalities increased with an increase of concentration or exposure time or both. Thus, highest mortality 90% was achieved at 840 watt power output and exposure time 50 second for both aforementioned species. Likewise, eucalyptus Eucalyptus camaldulensis, mint Mentha canadensis and myrtle Myrtus communis were studied for their toxicity effect on mortality of larval stage at three dosages (12500, 25000 and 50000) ppm for different exposure times (1, 2, 3 and 7) days. The larvae of khapra beetle were more resistant to the insecticidal activity of plant extracts in comparison with red flour beetle larvae. The LC50 values were varied in accordance to plant extracts types and concentrations within the four interval times of exposure. The LC50 values for both khapra & red flour beetles were (47234.07 & 5760.90) ppm respectively on black raisin after 7 days exposure to eucalyptus aqueous extract.

Genome-Wide Characterization and Expression Profiling of the AUXIN RESPONSE FACTOR (ARF) Gene Family in Eucalyptus grandis

PubMed Central

Yu, Hong; Soler, Marçal; Mila, Isabelle; San Clemente, Hélène; Savelli, Bruno; Dunand, Christophe; Paiva, Jorge A. P.; Myburg, Alexander A.; Bouzayen, Mondher; Grima-Pettenati, Jacqueline; Cassan-Wang, Hua

2014-01-01

Auxin is a central hormone involved in a wide range of developmental processes including the specification of vascular stem cells. Auxin Response Factors (ARF) are important actors of the auxin signalling pathway, regulating the transcription of auxin-responsive genes through direct binding to their promoters. The recent availability of the Eucalyptus grandis genome sequence allowed us to examine the characteristics and evolutionary history of this gene family in a woody plant of high economic importance. With 17 members, the E. grandis ARF gene family is slightly contracted, as compared to those of most angiosperms studied hitherto, lacking traces of duplication events. In silico analysis of alternative transcripts and gene truncation suggested that these two mechanisms were preeminent in shaping the functional diversity of the ARF family in Eucalyptus. Comparative phylogenetic analyses with genomes of other taxonomic lineages revealed the presence of a new ARF clade found preferentially in woody and/or perennial plants. High-throughput expression profiling among different organs and tissues and in response to environmental cues highlighted genes expressed in vascular cambium and/or developing xylem, responding dynamically to various environmental stimuli. Finally, this study allowed identification of three ARF candidates potentially involved in the auxin-regulated transcriptional program underlying wood formation. PMID:25269088

Numerical analysis of Eucalyptus grandis × E. urophylla heat-treatment: A dynamically detecting method of mass loss during the process

NASA Astrophysics Data System (ADS)

Zhao, Zijian; Ma, Qing; Mu, Jun; Yi, Songlin; He, Zhengbin

Eucalyptus particles, lamellas and boards were applied to explore a simply-implemented method with neglected heat and mass transfer to inspect the mass loss during the heat-treatment course. The results revealed that the mass loss of a certain period was theoretically the definite integration of loss rate to time in this period, and a monitoring model for mass loss speed was developed with the particles and validated with the lamellas and boards. The loss rate was correlated to the temperature and temperature-evolving speed in the model which was composed of three functions during different temperature-evolving period. The sample mass loss was calculated in the MATLAB for the lamellas and boards and the model was validated and adjusted based on the difference between the computed results and the practically measured loss values. The error ranges of the new models were -16.30% to 18.35% for wood lamellas and -9.86% to 6.80% for wood boards. This method made it possible to acquire the instantaneous loss value through continuously detecting the wood temperature evolution. This idea could provide a reference for the Eucalyptus heat-treatment to detect the treating course and control the final material characteristics.

Development of Genetic Markers in Eucalyptus Species by Target Enrichment and Exome Sequencing

PubMed Central

Dasgupta, Modhumita Ghosh; Dharanishanthi, Veeramuthu; Agarwal, Ishangi; Krutovsky, Konstantin V.

2015-01-01

The advent of next-generation sequencing has facilitated large-scale discovery, validation and assessment of genetic markers for high density genotyping. The present study was undertaken to identify markers in genes supposedly related to wood property traits in three Eucalyptus species. Ninety four genes involved in xylogenesis were selected for hybridization probe based nuclear genomic DNA target enrichment and exome sequencing. Genomic DNA was isolated from the leaf tissues and used for on-array probe hybridization followed by Illumina sequencing. The raw sequence reads were trimmed and high-quality reads were mapped to the E. grandis reference sequence and the presence of single nucleotide variants (SNVs) and insertions/ deletions (InDels) were identified across the three species. The average read coverage was 216X and a total of 2294 SNVs and 479 InDels were discovered in E. camaldulensis, 2383 SNVs and 518 InDels in E. tereticornis, and 1228 SNVs and 409 InDels in E. grandis. Additionally, SNV calling and InDel detection were conducted in pair-wise comparisons of E. tereticornis vs. E. grandis, E. camaldulensis vs. E. tereticornis and E. camaldulensis vs. E. grandis. This study presents an efficient and high throughput method on development of genetic markers for family– based QTL and association analysis in Eucalyptus. PMID:25602379

New Ceratocystis species from Eucalyptus and Cunninghamia in South China.

PubMed

Liu, FeiFei; Mbenoun, Michael; Barnes, Irene; Roux, Jolanda; Wingfield, Michael J; Li, GuoQing; Li, JieQiong; Chen, ShuaiFei

2015-06-01

During routine surveys for possible fungal pathogens in the rapidly expanding plantations of Eucalyptus and Cunninghamia lanceolata in China, numerous isolates of unknown species in the genus Ceratocystis (Microascales) were obtained from tree wounds. In this study we identified the Ceratocystis isolates from Eucalyptus and Cunninghamia in the GuangDong, GuangXi, FuJian and HaiNan Provinces of South China based on morphology and through comparisons of DNA sequence data for the ITS, partial β-tubulin and TEF-1α gene regions. Morphological and DNA sequence comparisons revealed two previously unknown species residing in the Indo-Pacific Clade. These are described here as Ceratocystis cercfabiensis sp. nov. and Ceratocystis collisensis sp. nov. Isolates of Ceratocystis cercfabiensis showed intragenomic variation in their ITS sequences and four strains were selected for cloning of the ITS gene region. Twelve ITS haplotypes were obtained from 17 clones selected for sequencing, differing in up to seven base positions and representing two separate phylogenetic groups. This is the first evidence of multiple ITS types in isolates of Ceratocystis residing in the Indo-Pacific Clade. Caution should thus be exercised when using the ITS gene region as a barcoding marker for Ceratocystis species in this clade. This study also represents the first record of a species of Ceratocystis from Cunninghamia.

Evolutionary History and Novel Biotic Interactions Determine Plant Responses to Elevated CO2 and Nitrogen Fertilization

PubMed Central

Wooliver, Rachel; Senior, John K.; Schweitzer, Jennifer A.; O'Reilly-Wapstra, Julianne M.; Langley, J. Adam; Chapman, Samantha K.; Bailey, Joseph K.

2014-01-01

A major frontier in global change research is predicting how multiple agents of global change will alter plant productivity, a critical component of the carbon cycle. Recent research has shown that plant responses to climate change are phylogenetically conserved such that species within some lineages are more productive than those within other lineages in changing environments. However, it remains unclear how phylogenetic patterns in plant responses to changing abiotic conditions may be altered by another agent of global change, the introduction of non-native species. Using a system of 28 native Tasmanian Eucalyptus species belonging to two subgenera, Symphyomyrtus and Eucalyptus, we hypothesized that productivity responses to abiotic agents of global change (elevated CO2 and increased soil N) are unique to lineages, but that novel interactions with a non-native species mediate these responses. We tested this hypothesis by examining productivity of 1) native species monocultures and 2) mixtures of native species with an introduced hardwood plantation species, Eucalyptus nitens, to experimentally manipulated soil N and atmospheric CO2. Consistent with past research, we found that N limits productivity overall, especially in elevated CO2 conditions. However, monocultures of species within the Symphyomyrtus subgenus showed the strongest response to N (gained 127% more total biomass) in elevated CO2 conditions, whereas those within the Eucalyptus subgenus did not respond to N. Root:shoot ratio (an indicator of resource use) was on average greater in species pairs containing Symphyomyrtus species, suggesting that functional traits important for resource uptake are phylogenetically conserved and explaining the phylogenetic pattern in plant response to changing environmental conditions. Yet, native species mixtures with E. nitens exhibited responses to CO2 and N that differed from those of monocultures, supporting our hypothesis and highlighting that both plant evolutionary history and introduced species will shape community productivity in a changing world. PMID:25479056

Improving the enzymatic hydrolysis of thermo-mechanical fiber from Eucalyptus urophylla by a combination of hydrothermal pretreatment and alkali fractionation.

PubMed

Sun, Shaoni; Cao, Xuefei; Sun, Shaolong; Xu, Feng; Song, Xianliang; Sun, Run-Cang; Jones, Gwynn Lloyd

2014-01-01

The recalcitrance of lignocellulosic biomass is a major limitation for its conversion into biofuels by enzymatic hydrolysis. The use of a pretreatment technology is an essential step to diminish biomass recalcitrance for bioethanol production. In this study, a two-step pretreatment using hydrothermal pretreatment at various temperatures and alkali fractionation was performed on eucalyptus fiber. The detailed chemical composition, physicochemical characteristics, and morphology of the pretreated fibers in each of the fractions were evaluated to advance the performance of eucalyptus fiber in enzymatic digestibility. The hydrothermal pretreatment (100 to 220°C) significantly degraded hemicelluloses, resulting in an increased crystallinity of the pretreated fibers. However, as the pretreatment temperature reached 240°C, partial cellulose was degraded, resulting in a reduced crystallinity of cellulose. As compared to the hydrothermal pretreatment alone, a combination of hydrothermal and alkali treatments significantly removed hemicelluloses and lignin, resulting in an improved enzymatic hydrolysis of the cellulose-rich fractions. As compared with the raw fiber, the enzymatic hydrolysis rate increased 1.1 to 8.5 times as the hydrothermal pretreatment temperature increased from 100 to 240°C. Interestingly, after a combination of hydrothermal pretreatment and alkali fractionation, the enzymatic hydrolysis rate increased 3.7 to 9.2 times. Taking into consideration the consumption of energy and the production of xylo-oligosaccharides and lignin, an optimum pretreatment condition was found to be hydrothermal pretreatment at 180°C for 30 min and alkali fractionation with 2% NaOH at 90°C for 2.5 h, in which 66.3% cellulose was converted into glucose by enzymatic hydrolysis. The combination of hydrothermal pretreatment and alkali fractionation was a promising method to remove hemicelluloses and lignin as well as overcome the biomass recalcitrance for enzymatic hydrolysis from eucalyptus fiber. In addition, the various techniques applied in this work constituted an efficient approach to understand the underlying chemical and morphological changes of the cellulose-rich fractions.

Parentage Reconstruction in Eucalyptus nitens Using SNPs and Microsatellite Markers: A Comparative Analysis of Marker Data Power and Robustness.

PubMed

Telfer, Emily J; Stovold, Grahame T; Li, Yongjun; Silva-Junior, Orzenil B; Grattapaglia, Dario G; Dungey, Heidi S

2015-01-01

Pedigree reconstruction using molecular markers enables efficient management of inbreeding in open-pollinated breeding strategies, replacing expensive and time-consuming controlled pollination. This is particularly useful in preferentially outcrossed, insect pollinated Eucalypts known to suffer considerable inbreeding depression from related matings. A single nucleotide polymorphism (SNP) marker panel consisting of 106 markers was selected for pedigree reconstruction from the recently developed high-density Eucalyptus Infinium SNP chip (EuCHIP60K). The performance of this SNP panel for pedigree reconstruction in open-pollinated progenies of two Eucalyptus nitens seed orchards was compared with that of two microsatellite panels with 13 and 16 markers respectively. The SNP marker panel out-performed one of the microsatellite panels in the resolution power to reconstruct pedigrees and out-performed both panels with respect to data quality. Parentage of all but one offspring in each clonal seed orchard was correctly matched to the expected seed parent using the SNP marker panel, whereas parentage assignment to less than a third of the expected seed parents were supported using the 13-microsatellite panel. The 16-microsatellite panel supported all but one of the recorded seed parents, one better than the SNP panel, although there was still a considerable level of missing and inconsistent data. SNP marker data was considerably superior to microsatellite data in accuracy, reproducibility and robustness. Although microsatellites and SNPs data provide equivalent resolution for pedigree reconstruction, microsatellite analysis requires more time and experience to deal with the uncertainties of allele calling and faces challenges for data transferability across labs and over time. While microsatellite analysis will continue to be useful for some breeding tasks due to the high information content, existing infrastructure and low operating costs, the multi-species SNP resource available with the EuCHIP60k, opens a whole new array of opportunities for high-throughput, genome-wide or targeted genotyping in species of Eucalyptus.

Functional relationships between wood structure and vulnerability to xylem cavitation in races of Eucalyptus globulus differing in wood density.

PubMed

Barotto, Antonio José; Monteoliva, Silvia; Gyenge, Javier; Martinez-Meier, Alejandro; Fernandez, María Elena

2018-02-01

Wood density can be considered as a measure of the internal wood structure, and it is usually used as a proxy measure of other mechanical and functional traits. Eucalyptus is one of the most important commercial forestry genera worldwide, but the relationship between wood density and vulnerability to cavitation in this genus has been little studied. The analysis is hampered by, among other things, its anatomical complexity, so it becomes necessary to address more complex techniques and analyses to elucidate the way in which the different anatomical elements are functionally integrated. In this study, vulnerability to cavitation in two races of Eucalyptus globulus Labill. with different wood density was evaluated through Path analysis, a multivariate method that allows evaluation of descriptive models of causal relationship between variables. A model relating anatomical variables with wood properties and functional parameters was proposed and tested. We found significant differences in wood basic density and vulnerability to cavitation between races. The main exogenous variables predicting vulnerability to cavitation were vessel hydraulic diameter and fibre wall fraction. Fibre wall fraction showed a direct impact on wood basic density and the slope of vulnerability curve, and an indirect and negative effect over the pressure imposing 50% of conductivity loss (P50) through them. Hydraulic diameter showed a direct negative effect on P50, but an indirect and positive influence over this variable through wood density on one hand, and through maximum hydraulic conductivity (ks max) and slope on the other. Our results highlight the complexity of the relationship between xylem efficiency and safety in species with solitary vessels such as Eucalyptus spp., with no evident compromise at the intraspecific level. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Wood CO(2) efflux and foliar respiration for Eucalyptus in Hawaii and Brazil.

PubMed

Ryan, Michael G; Cavaleri, Molly A; Almeida, Auro C; Penchel, Ricardo; Senock, Randy S; Luiz Stape, José

2009-10-01

We measured CO(2) efflux from wood for Eucalyptus in Hawaii for 7 years and compared these measurements with those on three- and four-and-a-half-year-old Eucalyptus in Brazil. In Hawaii, CO(2) efflux from wood per unit biomass declined approximately 10x from age two to age five, twice as much as the decline in tree growth. The CO(2) efflux from wood in Brazil was 8-10x lower than that for comparable Hawaii trees with similar growth rates. Growth and maintenance respiration coefficients calculated from Hawaii wood CO(2) efflux declined with tree age and size (the growth coefficient declined from 0.4 mol C efflux mol C(-1) wood growth at age one to 0.1 mol C efflux mol C(-1) wood growth at age six; the maintenance coefficient from 0.006 to 0.001 micromol C (mol C biomass)(-1) s(-1) at 20 degrees C over the same time period). These results suggest interference with CO(2) efflux through bark that decouples CO(2) efflux from respiration. We also compared the biomass fractions and wood CO(2) efflux for the aboveground woody parts for 3- and 7-year-old trees in Hawaii to estimate how focusing measurements near the ground might bias the stand-level estimates of wood CO(2) efflux. Three-year-old Eucalyptus in Hawaii had a higher proportion of branches < 0.5 cm in diameter and a lower proportion of stem biomass than did 7-year-old trees. Biomass-specific CO(2) efflux measured at 1.4 m extrapolated to the tree could bias tree level estimates by approximately 50%, assuming no refixation from bark photosynthesis. However, the bias did not differ for the two tree sizes. Foliar respiration was identical per unit nitrogen for comparable treatments in Brazil and Hawaii (4.2 micromol C mol N(-1) s(-1) at 20 degrees C).

Evolutionary history and novel biotic interactions determine plant responses to elevated CO2 and nitrogen fertilization.

PubMed

Wooliver, Rachel; Senior, John K; Schweitzer, Jennifer A; O'Reilly-Wapstra, Julianne M; Langley, J Adam; Chapman, Samantha K; Bailey, Joseph K

2014-01-01

A major frontier in global change research is predicting how multiple agents of global change will alter plant productivity, a critical component of the carbon cycle. Recent research has shown that plant responses to climate change are phylogenetically conserved such that species within some lineages are more productive than those within other lineages in changing environments. However, it remains unclear how phylogenetic patterns in plant responses to changing abiotic conditions may be altered by another agent of global change, the introduction of non-native species. Using a system of 28 native Tasmanian Eucalyptus species belonging to two subgenera, Symphyomyrtus and Eucalyptus, we hypothesized that productivity responses to abiotic agents of global change (elevated CO2 and increased soil N) are unique to lineages, but that novel interactions with a non-native species mediate these responses. We tested this hypothesis by examining productivity of 1) native species monocultures and 2) mixtures of native species with an introduced hardwood plantation species, Eucalyptus nitens, to experimentally manipulated soil N and atmospheric CO2. Consistent with past research, we found that N limits productivity overall, especially in elevated CO2 conditions. However, monocultures of species within the Symphyomyrtus subgenus showed the strongest response to N (gained 127% more total biomass) in elevated CO2 conditions, whereas those within the Eucalyptus subgenus did not respond to N. Root:shoot ratio (an indicator of resource use) was on average greater in species pairs containing Symphyomyrtus species, suggesting that functional traits important for resource uptake are phylogenetically conserved and explaining the phylogenetic pattern in plant response to changing environmental conditions. Yet, native species mixtures with E. nitens exhibited responses to CO2 and N that differed from those of monocultures, supporting our hypothesis and highlighting that both plant evolutionary history and introduced species will shape community productivity in a changing world.

Soil microbial community structure and diversity are largely influenced by soil pH and nutrient quality in 78-year-old tree plantations

NASA Astrophysics Data System (ADS)

Zhou, Xiaoqi; Guo, Zhiying; Chen, Chengrong; Jia, Zhongjun

2017-04-01

Forest plantations have been recognised as a key strategy management tool for stocking carbon (C) in soils, thereby contributing to climate warming mitigation. However, long-term ecological consequences of anthropogenic forest plantations on the community structure and diversity of soil microorganisms and the underlying mechanisms in determining these patterns are poorly understood. In this study, we selected 78-year-old tree plantations that included three coniferous tree species (i.e. slash pine, hoop pine and kauri pine) and a eucalypt species in subtropical Australia. We investigated the patterns of community structure, and the diversity of soil bacteria and eukaryotes by using high-throughput sequencing of 16S rRNA and 18S rRNA genes. We also measured the potential methane oxidation capacity under different tree species. The results showed that slash pine and Eucalyptus significantly increased the dominant taxa of bacterial Acidobacteria and the dominant taxa of eukaryotic Ascomycota, and formed clusters of soil bacterial and eukaryotic communities, which were clearly different from the clusters under hoop pine and kauri pine. Soil pH and nutrient quality indicators such as C : nitrogen (N) and extractable organic C : extractable organic N were key factors in determining the patterns of soil bacterial and eukaryotic communities between the different tree species treatments. Slash pine and Eucalyptus had significantly lower soil bacterial and eukaryotic operational taxonomical unit numbers and lower diversity indices than kauri pine and hoop pine. A key factor limitation hypothesis was introduced, which gives a reasonable explanation for lower diversity indices under slash pine and Eucalyptus. In addition, slash pine and Eucalyptus had a higher soil methane oxidation capacity than the other tree species. These results suggest that significant changes in soil microbial communities may occur in response to chronic disturbance by tree plantations, and highlight the importance of soil pH and physiochemical characteristics in microbially mediated ecological processes in forested soils.

Effect of Low pH and Aluminum Toxicity on the Photosynthetic Characteristics of Different Fast-Growing Eucalyptus Vegetatively Propagated Clones.

PubMed

Yang, Mei; Tan, Ling; Xu, Yuanyuan; Zhao, Yihui; Cheng, Fei; Ye, Shaoming; Jiang, Weixin

2015-01-01

Knowing how acid soils and aluminum in soils may limit the growth of Eucalyptus trees in plantations is important because these plantations grow in many tropical and subtropical regions. Seedlings of four vegetatively propagated Eucalyptus clones, E. grandis × E. urophylla 'GLGU9'(G9), E. grandis × E. urophylla 'GLGU12' (G12), E. urophylla × E. camaldulensis 'GLUC3' (G3) and E. urophylla 'GLU4'(G4), were subjected to liquid culture with Hoagland nutrient solution for 40 days, then treated with four different treatments of acid and aluminum for 1 day. The four treatments used either pH 3.0 or 4.0 with or without added aluminum (4.4 mM) in all possible combinations; a control used no added aluminum at pH 4.8. Subsequently, the photosynthetic parameters and morphology of leaves from eucalypt seedlings were determined and observed. The results showed that the tested chlorophyll content, net photosynthetic rate, transpiration rate and water use efficiency were apparently inhibited by aluminum. Under uniform Al concentration (4.4 mM), the Al-induced limitation to photosynthetic parameters increased with pH, indicating acid stimulation to Al toxicity. Among all treatments, the most significant reduction was found in the combination of pH 3.0 and 4.4 mM Al. The photosynthetic and transpiration rates showed similar trends with G9 > G12 > G3 > G4, suggesting that G9 and G12 had higher Al-tolerance than other two clones. Microscopic observation revealed changes in leaf morphology when exposed to Al stress; for example, a reduced thickness of leaf epidermis and palisade tissue, the descendant palisade tissue/spongy tissue ratio and leaf tissue looseness. Overall, the acid and aluminum stress exerted negative effects on the photosynthetic activity of eucalypt seedlings, but the differences in tolerance to Al toxicity between the clones were favorable, offering potential to improve Eucalyptus plantation productivity by selecting Al tolerant clones.

Long-term water stress leads to acclimation of drought sensitivity of photosynthetic capacity in xeric but not riparian Eucalyptus species

PubMed Central

Zhou, Shuang-Xi; Medlyn, Belinda E.; Prentice, Iain Colin

2016-01-01

Background and Aims Experimental drought is well documented to induce a decline in photosynthetic capacity. However, if given time to acclimate to low water availability, the photosynthetic responses of plants to low soil moisture content may differ from those found in short-term experiments. This study aims to test whether plants acclimate to long-term water stress by modifying the functional relationships between photosynthetic traits and water stress, and whether species of contrasting habitat differ in their degree of acclimation. Methods Three Eucalyptus taxa from xeric and riparian habitats were compared with regard to their gas exchange responses under short- and long-term drought. Photosynthetic parameters were measured after 2 and 4 months of watering treatments, namely field capacity or partial drought. At 4 months, all plants were watered to field capacity, then watering was stopped. Further measurements were made during the subsequent ‘drying-down’, continuing until stomata were closed. Key Results Two months of partial drought consistently reduced assimilation rate, stomatal sensitivity parameters (g1), apparent maximum Rubisco activity (Vcmax′) and maximum electron transport rate (Jmax′). Eucalyptus occidentalis from the xeric habitat showed the smallest decline in Vcmax′ and Jmax′; however, after 4 months, Vcmax′ and Jmax′ had recovered. Species differed in their degree of Vcmax′ acclimation. Eucalyptus occidentalis showed significant acclimation of the pre-dawn leaf water potential at which the Vcmax′ and ‘true’ Vcmax (accounting for mesophyll conductance) declined most steeply during drying-down. Conclusions The findings indicate carbon loss under prolonged drought could be over-estimated without accounting for acclimation. In particular, (1) species from contrasting habitats differed in the magnitude of V′cmax reduction in short-term drought; (2) long-term drought allowed the possibility of acclimation, such that V′cmax reduction was mitigated; (3) xeric species showed a greater degree of V′cmax acclimation; and (4) photosynthetic acclimation involves hydraulic adjustments to reduce water loss while maintaining photosynthesis. PMID:26493470

Shade images of forested areas obtained from Landsat MSS data

NASA Technical Reports Server (NTRS)

Shimabukuro, Yosio Edemir; Smith, James A.

1989-01-01

The objective of this report is to generate a shade (shadow) image of forested areas from Landsat MSS data by implementing a linear mixing model, where shadow is considered as one of the primary components in a pixel. The shade images are related to the observed variation in forest structure; i.e., the proportion of inferred shadow in a pixel is related to different forest ages, forest types, and tree crown cover. The constrained least-squares method is used to generate shade images for forest of eucalyptus and vegetation of 'cerrado' over the Itapeva study area in Brazil. The resulted shade images may explain the difference on ages for forest of eucalyptus and the difference on tree crown cover for vegetation of cerrado.

Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla x E. grandis leads to improved sugar release

DOE PAGES

Sykes, Robert W.; Gjersing, Erica L.; Foutz, Kirk; ...

2015-08-27

In this study, lignocellulosic materials provide an attractive replacement for food-based crops used to produce ethanol. Understanding the interactions within the cell wall is vital to overcome the highly recalcitrant nature of biomass. One factor imparting plant cell wall recalcitrance is lignin, which can be manipulated by making changes in the lignin biosynthetic pathway. In this study, eucalyptus down-regulated in expression of cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) or p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H, EC 1.14.13.36) were evaluated for cell wall composition and reduced recalcitrance.

Research on private cloud computing based on analysis on typical opensource platform: a case study with Eucalyptus and Wavemaker

NASA Astrophysics Data System (ADS)

Yu, Xiaoyuan; Yuan, Jian; Chen, Shi

2013-03-01

Cloud computing is one of the most popular topics in the IT industry and is recently being adopted by many companies. It has four development models, as: public cloud, community cloud, hybrid cloud and private cloud. Except others, private cloud can be implemented in a private network, and delivers some benefits of cloud computing without pitfalls. This paper makes a comparison of typical open source platforms through which we can implement a private cloud. After this comparison, we choose Eucalyptus and Wavemaker to do a case study on the private cloud. We also do some performance estimation of cloud platform services and development of prototype software as cloud services.

Use of dimethyldioxirane in the epoxidation of the main constituents of the essential oils obtained from Tagetes lucida, Cymbopogon citratus, Lippia alba and Eucalyptus citriodora.

PubMed

Veloza, Luz A; Orozco, Lina M; Sepúlveda-Arias, Juan C

2011-07-01

Dimethyldioxirane (DMDO), a widely used oxidant in organic synthesis is considered an environmentally friendly oxygen transfer reagent because acetone is the only byproduct formed in its oxidation reactions. This work describes the isolation of the main constituents (terpenes) in the essential oils obtained from Tagetes lucida, Cymbopogon citratus, Lippia alba and Eucalyptus citriodora, their epoxidation with DMDO in acetone solution and the characterization of the resulting epoxides by GC-MS (EI) and NMR. This is one of the first reports involving the application of dioxirane chemistry to essential oils in order to generate modified compounds with potential uses in several areas of medicine and industry.

Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus

PubMed Central

Inoue, Hiroyuki; Yano, Shinichi; Endo, Takashi; Sakaki, Tsuyoshi; Sawayama, Shigeki

2008-01-01

Background Lignocellulosic biomass such as wood is an attractive material for fuel ethanol production. Pretreatment technologies that increase the digestibility of cellulose and hemicellulose in the lignocellulosic biomass have a major influence on the cost of the subsequent enzymatic hydrolysis and ethanol fermentation processes. Pretreatments without chemicals such as acids, bases or organic solvents are less effective for an enzymatic hydrolysis process than those with chemicals, but they have a less negative effect on the environment. Results The enzymatic digestibility of eucalyptus was examined following a combined pretreatment without chemicals comprising a ball milling (BM) and hot-compressed water (HCW) treatment. The BM treatment simultaneously improved the digestibility of both glucan and xylan, and was effective in lowering the enzyme loading compared with the HCW treatment. The combination of HCW and BM treatment reduced the BM time. The eucalyptus treated with HCW (160°C, 30 minutes) followed by BM (20 minutes) had an approximately 70% yield of total sugar with a cellulase loading of 4 FPU/g substrate. This yield was comparable to the yields from samples treated with HCW (200°C, 30 minutes) or BM (40 minutes) hydrolyzed with 40 FPU/g substrate. Conclusion The HCW treatment is useful in improving the milling efficiency. The combined HCW-BM treatment can save energy and enzyme loading. PMID:18471309

Spatial patterns of a tropical tree species growing under an eucalyptus plantation in South-East Brazil.

PubMed

Higuchi, P; Silva, A C; Louzada, J N C; Machado, E L M

2010-05-01

The objectives of this study were to evaluate the influence of propagules source and the implication of tree size class on the spatial pattern of Xylopia brasiliensis Spreng. individuals growing under the canopy of an experimental plantation of eucalyptus. To this end, all individuals of Xylopia brasiliensis with diameter at soil height (dsh) > 1 cm were mapped in the understory of a 3.16 ha Eucalyptus spp. and Corymbia spp. plantation, located in the municipality of Lavras, SE Brazil. The largest nearby mature tree of X. brasiliensis was considered as the propagules source. Linear regressions were used to assess the influence of the distance of propagules source on the population parameters (density, basal area and height). The spatial pattern of trees was assessed through the Ripley K function. The overall pattern showed that the propagules source distance had strong influence over spatial distribution of trees, mainly the small ones, indicating that the closer the distance from the propagules source, the higher the tree density and the lower the mean tree height. The population showed different spatial distribution patterns according to the spatial scale and diameter class considered. While small trees tended to be aggregated up to around 80 m, the largest individuals were randomly distributed in the area. A plausible explanation for observed patterns might be limited seed rain and intra-population competition.

Proteomic plasticity of two Eucalyptus genotypes under contrasted water regimes in the field.

PubMed

Bedon, Frank; Villar, Emilie; Vincent, Delphine; Dupuy, Jean-William; Lomenech, Anne-Marie; Mabialangoma, André; Chaumeil, Philippe; Barré, Aurélien; Plomion, Christophe; Gion, Jean-Marc

2012-04-01

Water deficit affects tree growth and limits wood production. In an attempt to identify the molecular triggers of adaptation mechanisms to water deficit in Eucalyptus, we investigated protein expression patterns of two ecophysiologically contrasted Eucalyptus genotypes. They were grown in the field in either natural conditions or irrigated for 7 weeks during the dry season in the Republic of Congo. At the phenotypic level, genotype (G), treatment (T) and/or G × T interaction effects were observed for above- and below-ground biomass-related traits. At the molecular level, changes in protein abundance were recorded in leaves (acidic pH 4-7, and basic pH 7-11, proteomes) and stems (acidic proteome) using two-dimensional gel electrophoresis (2-DE). One third of the detected protein spots displayed significant G, T and/or G × T effects, and 158 of them were identified by tandem mass spectrometry (LC-MS/MS) analysis. Thus, several proteins whose molecular plasticity was genetically controlled (i.e. G × T effect) were revealed, highlighting adaptive mechanisms to water deficit specific to each genotype, namely cell wall modification, cell detoxification and osmoregulation. Transcript abundances corresponding to G × T proteins were also investigated by quantitative RT-PCR. These proteins represent relevant targets to improve drought resistance in this ecologically and economically important forest tree genus. © 2011 Blackwell Publishing Ltd.

Response of soil respiration and ecosystem carbon budget to vegetation removal in Eucalyptus plantations with contrasting ages.

PubMed

Wu, Jianping; Liu, Zhanfeng; Huang, Guomin; Chen, Dima; Zhang, Weixin; Shao, Yuanhu; Wan, Songze; Fu, Shenglei

2014-09-02

Reforested plantations have substantial effects on terrestrial carbon cycling due to their large coverage area. Although understory plants are important components of reforested plantations, their effects on ecosystem carbon dynamics remain unclear. This study was designed to investigate the effects of vegetation removal/understory removal and tree girdling on soil respiration and ecosystem carbon dynamics in Eucalyptus plantations of South China with contrasting ages (2 and 24 years old). We conducted a field manipulation experiment from 2008 to 2009. Understory removal reduced soil respiration in both plantations, whereas tree girdling decreased soil respiration only in the 2-year-old plantations. The net ecosystem production was approximately three times greater in the 2-year-old plantations (13.4 t C ha(-1) yr(-1)) than in the 24-year-old plantations (4.2 t C h(-1) yr(-1)). The biomass increase of understory plants was 12.6 t ha(-1) yr(-1) in the 2-year-old plantations and 2.9 t ha(-1) yr(-1) in the 24-year-old plantations, accounting for 33.9% nd 14.1% of the net primary production, respectively. Our findings confirm the ecological importance of understory plants in subtropical plantations based on the 2 years of data. These results also indicate that Eucalyptus plantations in China may be an important carbon sink due to the large plantation area.

Stomatal structure and physiology do not explain differences in water use among montane eucalypts.

PubMed

Gharun, Mana; Turnbull, Tarryn L; Pfautsch, Sebastian; Adams, Mark A

2015-04-01

Understanding the regulation of water use at the whole-tree scale is critical to advancing the utility of physiological ecology, for example in its role in predictive hydrology of forested catchments. For three eucalypt species that dominate high-elevation catchments in south-eastern Australia, we examined if whole-tree water use could be related to three widely discussed regulators of water use: stomatal anatomy, sensitivity of stomata [i.e. stomatal conductance (g(s))] to environmental influences, and sapwood area. While daily tree water use varied sixfold among species, sap velocity and sapwood area varied in parallel. Combined, stomatal structure and physiology could not explain differences in species-specific water use. Species which exhibited the fastest (Eucalyptus delegatensis) and slowest (Eucalyptus pauciflora) rates of water use both exhibited greater capacity for physiological control of g(s) [indicated by sensitivity to vapour pressure deficit (VPD)] and a reduced capacity to limit g(s) anatomically [indicated by greater potential g(s) (g(max))]. Conversely, g(s) was insensitive to VPD and g(max) was lowest for Eucalyptus radiata, the species showing intermediate rates of water use. Improved knowledge of stomatal anatomy will help us to understand the capacity of species to regulate leaf-level water loss, but seems likely to remain of limited use for explaining rates of whole-tree water use in montane eucalypts at the catchment scale.

Skin-Applied Repellent Ingredients

EPA Pesticide Factsheets

Active ingredients in EPA-registered insect repellents include catnip oil, oil of citronella, DEET, IR 3535, picaridin, oil of lemon eucalyptus, and 2-undecanone. Find fact sheets and pesticide regulatory information.

Integrated approach for selecting efficient Saccharomyces cerevisiae for industrial lignocellulosic fermentations: Importance of yeast chassis linked to process conditions.

PubMed

Costa, Carlos E; Romaní, Aloia; Cunha, Joana T; Johansson, Björn; Domingues, Lucília

2017-03-01

In this work, four robust yeast chassis isolated from industrial environments were engineered with the same xylose metabolic pathway. The recombinant strains were physiologically characterized in synthetic xylose and xylose-glucose medium, on non-detoxified hemicellulosic hydrolysates of fast-growing hardwoods (Eucalyptus and Paulownia) and agricultural residues (corn cob and wheat straw) and on Eucalyptus hydrolysate at different temperatures. Results show that the co-consumption of xylose-glucose was dependent on the yeast background. Moreover, heterogeneous results were obtained among different hydrolysates and temperatures for each individual strain pointing to the importance of designing from the very beginning a tailor-made yeast considering the specific raw material and process. Copyright © 2016 Elsevier Ltd. All rights reserved.

Non-isothermal crystallization kinetics of eucalyptus lignosulfonate/polyvinyl alcohol composite.

PubMed

Ye, De-Zhan; Zhang, Xi; Gu, Shaojin; Zhou, Yingshan; Xu, Weilin

2017-04-01

The nonisothermal crystallinization kinetic was performed on Polyvinyl alcohol (PVA) mixed with eucalyptus lignosulfonate calcuim (HLS) as the biobased thermal stabilizer, which was systematically analyzed based on Jeziorny model, Ozawa equation and the Mo method. The results indicated that the entire crystallization process took place through two main stages involving the primary and secondary crystallization processes. The Mo method described nonisothermal crystallization behavior well. Based on the results of the half time for completing crystallization, k c value in Jeziorny model, F(T) value in Mo method and crystallization activation energy, it was concluded that low loading of HLS accelerated PVA crystallization process, however, the growth rate of PVA crystallization was impeded at high content of HLS. Copyright © 2017 Elsevier B.V. All rights reserved.

The role of forest in runoff generation in a suburban catchment

NASA Astrophysics Data System (ADS)

Ferreira, C. S. S.; Soares, D.; Soares, A. J. D.; Coelho, C. O. A.; Steenhuis, T. S.; Keizer, J. J.; Walsh, R. P. D.

2012-04-01

Forests play an important role in the water cycle, particularly through their influence on infiltration and evapotranspiration processes. Removing forest for urban growth will affect the hydrological cycle, but to what degree is not known. To improve the knowledge about the role of forest areas in the catchment surface runoff, a total of nine runoff plots (16m2) was installed in the three predominant woodland types found in the small Ribeira dos Covões catchment (620ha), located in a rapid urbanizing area in central Portugal. The three representative study sites comprised: (i) a dense eucalyptus stand on a sandy-loam soil overlying sandstone; (ii) a open eucalyptus stand dominated by dense shrub vegetation, also on a sandy-loam soil overlying sandstone; (iii) a Mediterranean oak stand on a loamy soil overlying limestone. The three plots at each site were bounded by metal sheets and their outlets were connected to a modified Gerlach through for sediments retention and, subsequently, a tipping-bucket device and a tank for recording and collecting the runoff. The overland flow generated by the plots was monitored for almost one year. In addition, soil moisture content was measured automatically at 0-2, 5-10 and 15-20cm soil depth using 5 sensors per plot. Furthermore, soil water repellency was repeatedly measured on the field, through ethanol percentage method. In the dense eucalyptus forest the soil is hydrophobic during most of the year, just vanished after severe rainfall events. This reflects on low soil moisture content that reached 37% during wet periods. In this area, with an average slope of 20°±5°, the runoff coefficient ranged between 0.0% (for a 3mm rainfall event) and 2.2% (for a 23mm rainfall during hydrophobic conditions). In general, the runoff was higher when the soil was extremely repellent, but it also increased with soil moisture rise when the repellence was absent (reaching 0.6%). In the open eucalyptus forest, hydrophobicity is also presented but it is absent for a longer period comparing with the dense eucalyptus. Nonetheless, the soil moisture content is always lower, with a maximum of 26%. Despite the higher slope (27°±1°), this is thought to be a consequence of the very dense shrub cover, which can explain the lower runoff coefficients (maximum of 0.5%). In these plots, runoff increases with soil moisture. On the other hand, in oak forest the soil is mostly hydrophilic, this indicates the role of vegetation type on water repellence. The soil moisture is higher along the year (35% - 66%), not only due to hydrophobicity nonexistence but also with lower slope (17°±5°). On this forest, overland-flow is almost absent (attaining 0.3%) and increases with soil moisture. The low runoff coefficients show that even when the soil is hydrophobic, water is able to infiltrate to the subsurface through preferential flows. The results confirm the widespread notion that forest areas increase infiltration and, thereby, reduce flood risk. Nonetheless, eucalyptus stand is little suitable as forest cover, comparing with natural oak forest, to promote water infiltration. This knowledge can aid decision-makers dealing with urban planning.

Eucalyptus oil overdose

MedlinePlus

... liniments Diaper rash creams Inhalers to relieve nasal congestion Medicine for sore gums, mouth, and throat Mouthwashes ... IV) Medicines to treat symptoms Activated charcoal Laxative Tube through the nose into the stomach to wash ...

Combined effect of pulse density and grid cell size on predicting and mapping aboveground carbon in fast-growing Eucalyptus forest plantation using airborne LiDAR data.

PubMed

Silva, Carlos Alberto; Hudak, Andrew Thomas; Klauberg, Carine; Vierling, Lee Alexandre; Gonzalez-Benecke, Carlos; de Padua Chaves Carvalho, Samuel; Rodriguez, Luiz Carlos Estraviz; Cardil, Adrián

2017-12-01

LiDAR remote sensing is a rapidly evolving technology for quantifying a variety of forest attributes, including aboveground carbon (AGC). Pulse density influences the acquisition cost of LiDAR, and grid cell size influences AGC prediction using plot-based methods; however, little work has evaluated the effects of LiDAR pulse density and cell size for predicting and mapping AGC in fast-growing Eucalyptus forest plantations. The aim of this study was to evaluate the effect of LiDAR pulse density and grid cell size on AGC prediction accuracy at plot and stand-levels using airborne LiDAR and field data. We used the Random Forest (RF) machine learning algorithm to model AGC using LiDAR-derived metrics from LiDAR collections of 5 and 10 pulses m -2 (RF5 and RF10) and grid cell sizes of 5, 10, 15 and 20 m. The results show that LiDAR pulse density of 5 pulses m -2 provides metrics with similar prediction accuracy for AGC as when using a dataset with 10 pulses m -2 in these fast-growing plantations. Relative root mean square errors (RMSEs) for the RF5 and RF10 were 6.14 and 6.01%, respectively. Equivalence tests showed that the predicted AGC from the training and validation models were equivalent to the observed AGC measurements. The grid cell sizes for mapping ranging from 5 to 20 also did not significantly affect the prediction accuracy of AGC at stand level in this system. LiDAR measurements can be used to predict and map AGC across variable-age Eucalyptus plantations with adequate levels of precision and accuracy using 5 pulses m -2 and a grid cell size of 5 m. The promising results for AGC modeling in this study will allow for greater confidence in comparing AGC estimates with varying LiDAR sampling densities for Eucalyptus plantations and assist in decision making towards more cost effective and efficient forest inventory.

Antifungal activity of essential oils on two Venturia inaequalis strains with different sensitivities to tebuconazole.

PubMed

Muchembled, Jérôme; Deweer, Caroline; Sahmer, Karin; Halama, Patrice

2017-11-02

The antifungal activity of seven essential oils (eucalyptus, clove, mint, oregano, savory, tea tree, and thyme) was studied on Venturia inaequalis, the fungus responsible for apple scab. The composition of the essential oils was checked by gas chromatography-mass spectrometry. Each essential oil had its main compound. Liquid tests were performed to calculate the IC 50 of essential oils as well as their majority compounds. The tests were made on two strains with different sensitivities to tebuconazole: S755, the sensitive strain, and rs552, the strain with reduced sensitivity. Copper sulfate was selected as the reference mineral fungicidal substance. IC 50 with confidence intervals were calculated after three independent experiments. The results showed that all essential oils and all major compounds had in vitro antifungal activities. Moreover, it was highlighted that the effectiveness of four essential oils (clove, eucalyptus, mint, and savory) was higher than copper sulfate on both strains. For each strain, the best activity was obtained using clove and eucalyptus essential oils. For clove, the IC 50 obtained on the sensitive strain (5.2 mg/L [4.0-6.7 mg/L]) was statistically lower than the IC 50 of reduced sensitivity strain (14 mg/L [11.1-17.5 mg/L]). In contrast, for eucalyptus essential oil, the IC 50 were not different with respectively 9.4-13.0 and 12.2-17.9 mg/L for S755 and rs552 strains. For mint, origano, savory, tea tree, and thyme, IC 50 were always the best on rs552 strain. The majority compounds were not necessarily more efficient than their corresponding oils; only eugenol (for clove) and carvacrol (for oregano and savory) seemed to be more effective on S755 strain. On the other hand, rs552 strain seemed to be more sensitive to essential oils than S755 strain. In overall, it was shown that essential oils have different antifungal activities but do not have the same antifungal activities depending on the fungus strain used.

Effect of Low pH and Aluminum Toxicity on the Photosynthetic Characteristics of Different Fast-Growing Eucalyptus Vegetatively Propagated Clones

PubMed Central

Yang, Mei; Tan, Ling; Xu, Yuanyuan; Zhao, Yihui; Cheng, Fei; Ye, Shaoming; Jiang, Weixin

2015-01-01

Knowing how acid soils and aluminum in soils may limit the growth of Eucalyptus trees in plantations is important because these plantations grow in many tropical and subtropical regions. Seedlings of four vegetatively propagated Eucalyptus clones, E. grandis × E. urophylla ‘GLGU9’(G9), E. grandis × E. urophylla ‘GLGU12’ (G12), E. urophylla × E. camaldulensis ‘GLUC3’ (G3) and E. urophylla ‘GLU4’(G4), were subjected to liquid culture with Hoagland nutrient solution for 40 days, then treated with four different treatments of acid and aluminum for 1 day. The four treatments used either pH 3.0 or 4.0 with or without added aluminum (4.4 mM) in all possible combinations; a control used no added aluminum at pH 4.8. Subsequently, the photosynthetic parameters and morphology of leaves from eucalypt seedlings were determined and observed. The results showed that the tested chlorophyll content, net photosynthetic rate, transpiration rate and water use efficiency were apparently inhibited by aluminum. Under uniform Al concentration (4.4 mM), the Al-induced limitation to photosynthetic parameters increased with pH, indicating acid stimulation to Al toxicity. Among all treatments, the most significant reduction was found in the combination of pH 3.0 and 4.4 mM Al. The photosynthetic and transpiration rates showed similar trends with G9 > G12 > G3 > G4, suggesting that G9 and G12 had higher Al-tolerance than other two clones. Microscopic observation revealed changes in leaf morphology when exposed to Al stress; for example, a reduced thickness of leaf epidermis and palisade tissue, the descendant palisade tissue/spongy tissue ratio and leaf tissue looseness. Overall, the acid and aluminum stress exerted negative effects on the photosynthetic activity of eucalypt seedlings, but the differences in tolerance to Al toxicity between the clones were favorable, offering potential to improve Eucalyptus plantation productivity by selecting Al tolerant clones. PMID:26090998

Eucalyptus water use greater than rainfall input - possible explanation from southern India

NASA Astrophysics Data System (ADS)

Calder, I. R.; Rosier, P. T. W.; Prasanna, K. T.; Parameswarappa, S.

Hydrological and silvicultural studies carried out in southern India on the effects of plantations of Eucalyptus and other fast growing exotic tree species have determined the impacts of these plantations on water resources, erosion, soil nutrient status and growth rates at sites of differing rainfall and soil depth in Karnataka. Whilst providing new information on these issues, the studies also raised two important questions: what was the explanation for the anomalous result that the water use of 3400 mm from Eucalyptus plantations at Hosakote over a three year period exceeded the rainfall of 2100 mm over the same period and why were growth rates of woodlots on most farmer's fields higher than those of plantations on land owned by the Karnataka Forest Department? The records of the soil moisture depletion patterns under these plantations from the day of planting provide the basis for the answers to both questions: i) whilst roots are penetrating into deeper soil layers, they are able to extract from a reservoir of water additional to that available from the rainfall each year, ii) farmer's land on which short rooted agricultural crops have been grown previously is likely to have a much higher soil water status than land previously under forest or scrub vegetation. These new studies have also established that the development of the drying front under the Eucalyptus camaldulensis plantations is very rapid, indicating average root extension rates in excess of 2.5 m per year, whilst those under Tectona grandis and Artocarpus heterophyllus advanced at approximately half the rate. These results have obvious implications for the long term sustainability of growth rates from these plantations and the recharge of groundwater. The authors believe that this study may be the first to report neutron probe soil moisture depletion observations, from the date of planting, beneath tree plantations in a dry climate. The extent to which the roots were able to penetrate raises the question of whether other studies, which have estimated water use from soil moisture observations in dry climates, may have seriously underestimated both the actual soil moisture depletion and the water use through having soil moisture measurements located to insufficient depth.

Long-term water stress leads to acclimation of drought sensitivity of photosynthetic capacity in xeric but not riparian Eucalyptus species.

PubMed

Zhou, Shuang-Xi; Medlyn, Belinda E; Prentice, Iain Colin

2016-01-01

Experimental drought is well documented to induce a decline in photosynthetic capacity. However, if given time to acclimate to low water availability, the photosynthetic responses of plants to low soil moisture content may differ from those found in short-term experiments. This study aims to test whether plants acclimate to long-term water stress by modifying the functional relationships between photosynthetic traits and water stress, and whether species of contrasting habitat differ in their degree of acclimation. Three Eucalyptus taxa from xeric and riparian habitats were compared with regard to their gas exchange responses under short- and long-term drought. Photosynthetic parameters were measured after 2 and 4 months of watering treatments, namely field capacity or partial drought. At 4 months, all plants were watered to field capacity, then watering was stopped. Further measurements were made during the subsequent 'drying-down', continuing until stomata were closed. Two months of partial drought consistently reduced assimilation rate, stomatal sensitivity parameters (g1), apparent maximum Rubisco activity (V'(cmax)) and maximum electron transport rate (J'(max)). Eucalyptus occidentalis from the xeric habitat showed the smallest decline in V'(cmax) and J'(max); however, after 4 months, V'(cmax) and J'(max) had recovered. Species differed in their degree of V'(cmax) acclimation. Eucalyptus occidentalis showed significant acclimation of the pre-dawn leaf water potential at which the V'(cmax) and 'true' V(cmax) (accounting for mesophyll conductance) declined most steeply during drying-down. The findings indicate carbon loss under prolonged drought could be over-estimated without accounting for acclimation. In particular, (1) species from contrasting habitats differed in the magnitude of V'(cmax) reduction in short-term drought; (2) long-term drought allowed the possibility of acclimation, such that V'(cmax) reduction was mitigated; (3) xeric species showed a greater degree of V'(cmax) acclimation; and (4) photosynthetic acclimation involves hydraulic adjustments to reduce water loss while maintaining photosynthesis. © The Author 2015. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

14. EAST ELEVATION, COTTAGE. EXTERIOR NEARLY RESTORED. INTERIOR UNDERGOING RESTORATION. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. EAST ELEVATION, COTTAGE. EXTERIOR NEARLY RESTORED. INTERIOR UNDERGOING RESTORATION. EUCALYPTUS TREE PLANTED BY GERTRUDE KEIL PLANNED FOR REMOVAL. - Gold Ridge Farm, 7777 Bodega Avenue, Sebastopol, Sonoma County, CA

Eucalypt Improvement for California: Progress and Plans

Treesearch

F. Thomas Ledig

1983-01-01

Six promising eucalypt species, suitable for fiber or biomass production, have been identified with some confidence: Eucalyptus camaldulensis, E. dalrympleana, E. viminalis, E. nitens, E. globulus, and E. grandis. The Forest Service...

Contextual view showing drainage culvert in foreground boarding east side ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Contextual view showing drainage culvert in foreground boarding east side of knoll with eucalyptus windbreak. Camera facing 278" southwest. - Goerlitz House, 9893 Highland Avenue, Rancho Cucamonga, San Bernardino County, CA

Furfural production from Eucalyptus wood using an Acidic Ionic Liquid.

PubMed

Peleteiro, Susana; Santos, Valentín; Garrote, Gil; Parajó, Juan Carlos

2016-08-01

Eucalyptus globulus wood samples were treated with hot, compressed water to separate hemicelluloses (as soluble saccharides) from a solid phase mainly made up of cellulose and lignin. The liquid phase was dehydrated, and the resulting solids (containing pentoses as well as poly- and oligo- saccharides made up of pentoses) were dissolved and reacted in media containing an Acidic Ionic Liquid (1-butyl-3-methylimidazolium hydrogen sulfate) and a co-solvent (dioxane). The effects of the reaction time on the product distribution were studied at temperatures in the range 120-170°C for reaction times up to 8h, and operational conditions leading to 59.1% conversion of the potential substrates (including pentoses and pentose structural units in oligo- and poly- saccharides) into furfural were identified. Copyright © 2016 Elsevier Ltd. All rights reserved.

Macrocarpal C isolated from Eucalyptus globulus inhibits dipeptidyl peptidase 4 in an aggregated form.

PubMed

Kato, Eisuke; Kawakami, Kazuhiro; Kawabata, Jun

2018-12-01

Dipeptidyl peptidase 4 (DPP-4) inhibitors are used for the treatment of type-2 diabetes mellitus. Various synthetic inhibitors have been developed to date, and plants containing natural DPP-4 inhibitors have also been identified. Here, 13 plant samples were tested for their DPP-4 inhibitory activity. Macrocarpals A-C were isolated from Eucalyptus globulus through activity-guided fractionation and shown to be DPP-4 inhibitors. Of these, macrocarpal C showed the highest inhibitory activity, demonstrating an inhibition curve characterised by a pronounced increase in activity within a narrow concentration range. Evaluation of macrocarpal C solution by turbidity, nuclear magnetic resonance spectroscopy and mass spectrometry indicated its aggregation, which may explain the characteristics of the inhibition curve. These findings will be valuable for further study of potential small molecule DPP-4 inhibitors.

Characterisation of PM 10 emissions from woodstove combustion of common woods grown in Portugal

NASA Astrophysics Data System (ADS)

Gonçalves, Cátia; Alves, Célia; Evtyugina, Margarita; Mirante, Fátima; Pio, Casimiro; Caseiro, Alexandre; Schmidl, Christoph; Bauer, Heidi; Carvalho, Fernando

2010-11-01

A series of source tests was performed to evaluate the chemical composition of particle emissions from the woodstove combustion of four prevalent Portuguese species of woods: Pinus pinaster (maritime pine), Eucalyptus globulus (eucalyptus), Quercus suber (cork oak) and Acacia longifolia (golden wattle). Analyses included water-soluble ions, metals, radionuclides, organic and elemental carbon (OC and EC), humic-like substances (HULIS), cellulose and approximately l80 organic compounds. Particle (PM 10) emission factors from eucalyptus and oak were higher than those from pine and acacia. The carbonaceous matter represented 44-63% of the particulate mass emitted during the combustion process, regardless of species burned. The major organic components of smoke particles, for all the wood species studied, with the exception of the golden wattle (0.07-1.9% w/w), were anhydrosugars (0.2-17% w/w). Conflicting with what was expected, only small amounts of cellulose were found in wood smoke. As for HULIS, average particle mass concentrations ranged from 1.5% to 3.0%. The golden wattle wood smoke presented much higher concentrations of ions and metal species than the emissions from the other wood types. The results of the analysis of radionuclides revealed that the 226Ra was the naturally occurring radionuclide more enriched in PM 10. The chromatographically resolved organics included n-alkanes, n-alkenes, PAH, oxygenated PAH, n-alkanals, ketones, n-alkanols, terpenoids, triterpenoids, phenolic compounds, phytosterols, alcohols, n-alkanoic acids, n-di-acids, unsaturated acids and alkyl ester acids.

Larvicidal effect of Eucalyptus grandis essential oil and turpentine and their major components on Aedes aegypti larvae.

PubMed

Lucia, Alejandro; Gonzalez Audino, Paola; Seccacini, Emilia; Licastro, Susana; Zerba, Eduardo; Masuh, Hector

2007-09-01

In the search for new alternatives for the control of Aedes aegypti the larvicidal activity of Eucalyptus grandis essential oil and pine resin essential oil (turpentine) and their major components (alpha- and beta-pinene and 1,8-cineole) was determined. Gas chromatography-mass spectroscopy analysis of E. grandis essential oil revealed that its major components are alpha-pinene and 1,8-cineole. Similar analysis of turpentine obtained by distillation of the resin pitch of conifers showed that alpha- and beta-pinene are the only major components. Third and early 4th instars of the CIPEIN-susceptible strain of Ae. aegypti were exposed to acetonic solutions of E. grandis essential oil, turpentine, and their major components for 24 h. Turpentine, with an LC50 of 14.7 ppm, was more active than the essential oil of E. grandis (LC50: 32.4 ppm). Larvicidal activity of the essential oil components showed that alpha- and beta-pinene present low LC50 values (15.4 and 12.1 ppm, respectively), whereas pure 1,8-cineole showed an LC50 of 57.2 ppm. These results suggest that alpha-pinene in E. grandis and alpha- and beta-pinene in turpentine serve as the principal larvicidal components of both oils. Results obtained on larvicidal effects of essential oil of Eucalyptus grandis and turpentine could be considered a contribution to the search for new biodegradable larvicides of natural origin.

Effects of Alien Plants on Ecosystem Structure and Functioning and Implications for Restoration: Insights from Three Degraded Sites in South African Fynbos

NASA Astrophysics Data System (ADS)

Gaertner, Mirijam; Richardson, David M.; Privett, Sean D. J.

2011-07-01

We investigated the type and extent of degradation at three sites on the Agulhas Plain, South Africa: an old field dominated by the alien grass Pennisetum clandestinum Pers . (kikuyu), an abandoned Eucalyptus plantation, and a natural fynbos community invaded by nitrogen fixing—Australian Acacia species. These forms of degradation are representative of many areas in the region. By identifying the nature and degree of ecosystem degradation we aimed to determine appropriate strategies for restoration in this biodiversity hotspot. Vegetation surveys were conducted at degraded sites and carefully selected reference sites. Soil-stored propagule seed banks and macro- and micro-soil nutrients were determined. Species richness, diversity and native cover under Eucalyptus were extremely low compared to the reference site and alterations of the soil nutrients were the most severe. The cover of indigenous species under Acacia did not differ significantly from that in reference sites, but species richness was lower under Acacia and soils were considerably enriched. Native species richness was much lower in the kikuyu site, but soil nutrient status was similar to the reference site. Removal of the alien species alone may be sufficient to re-initiate ecosystem recovery at the kikuyu site, whereas active restoration is required to restore functioning ecosystems dominated by native species in the Acacia thicket and the Eucalyptus plantation. To restore native plant communities we suggest burning, mulching with sawdust and sowing of native species.

Biocontrol of Botrytis cinerea and Calonectria gracilis by eucalypts growth promoters Bacillus spp.

PubMed

Paz, Isabel Cristina Padula; Santin, Rita de Cássia Madail; Guimarães, Alexandre Martins; Rosa, Osmar Paulo Pereira da; Quecine, Maria Carolina; Silva, Michele de Cássia Pereira E; Azevedo, João Lúcio; Matsumura, Aida Terezinha Santos

2018-05-17

The clonal Eucalyptus plants are commonly obtained by vegetative propagation under a protected environment. This system improves the Botrytis cinerea and Calonectria spp infection on the young eucalypts plantings, resulting gray mold and cutting rot respectively. Currently, the unique available control method is based on chemicals. As alternative, novel methods to manage plant diseases, endophytic microorganisms could be an interesting alternative. Thus, we aimed to evaluate endophytic Bacillus isolated from eucalypts as a biocontrol agent against Botrytis cinerea and Calonectria gracilis, important fungal pathogens in the greenhouse, using clonal plantlets of E. urograndis. Eight endophytic strains of Bacillus, previously described as eucalyptus growth promoters, were evaluated in vitro and in vivo against Botrytis cinerea and Calonectria gracilis. The diffusible metabolites assay showed the potential of endophytic Bacillus to decrease the growth of both pathogens. Differences in the susceptibility of the pathogens to bacterial volatile metabolites were observed, B. cinerea showed more susceptible than Calonectria gracilis. In vivo assays, Bacillus amyloliquefaciens EUCB 10 demonstrated better overall reductions in these diseases. Based on the results obtained from the in vitro and in vivo analyses, we suggest that the endophytic B. amyloliquefaciens strain EUCB 10 constitutes a promising biocontrol agent against B. cinerea and Calonectria gracilis. Furthermore, this is the first reporting of B. amyloliquefaciens previously describe as plant growth promoter and also as potential control agent of B. cinerea and Calonectria gracilis to eucalyptus. Copyright © 2018 Elsevier Ltd. All rights reserved.

Nematodes from galls on Myrtaceae. IV. Fergusobia from flat leaf galls on Eucalyptus and Corymbia, with descriptions of two new species.

PubMed

Davies, Kerrie A; Giblin-Davis, Robin M; Ye, Weimin; Taylor, Gary S; Thomas, W Kelley

2013-11-26

Two new species of Fergusobia are described. Both were collected from flat leaf galls from South Australia, one on Eucalyptus microcarpa and the other on E. porosa. Fergusobia microcarpae n. sp. Davies is characterised by the combination of a C-shaped parthenogenetic female with a short, broadly rounded conoid tail, an arcuate to open C-shaped infective female with an hemispherical tail tip, and arcuate to J-shaped males with angular spicules and short peloderan bursa. Fergusobia porosae n. sp. Davies is similar in having an arcuate to C-shaped parthenogenetic female with a small conoid tail, an almost straight to arcuate infective female with an hemispherical tail tip, and males that are almost straight to barely J-shaped with angular spicules and short peloderan bursa. They differ in that the bodies of parthenogenetic and infective females of F. microcarpae n. sp. are more curved than in F. porosae n. sp. Other known similar forms of Fergusobia/Fergusonina galls are outlined and the larval shield morphologies of their associated mutualistic Fergusonina fly species are discussed where known. An inventory of all known Fergusobia/Fergusonina associations from flat leaf galls from Corymbia spp. and Eucalyptus spp. is presented. Relationships of Fergusobia nematodes were inferred from analysis of sequences of 28S rDNA D2/D3 domains and a portion of mitochondrial DNA cytochrome oxidase subunit I (mtCOI). Nematodes from flat leaf galls appeared in two clades. 

Quality parameters and antioxidant and antibacterial properties of some Mexican honeys.

PubMed

Rodríguez, Beatriz A; Mendoza, Sandra; Iturriga, Montserrat H; Castaño-Tostado, Eduardo

2012-01-01

A total of 14 Mexican honeys were screened for quality parameters including color, moisture, proline, and acidity. Antioxidant properties of complete honey and its methanolic extracts were evaluated by the DPPH, ABTS, and FRAP assays. In addition, the antimicrobial activity of complete honeys against Bacillus cereus ATCC 10876, Listeria monocytogenes Scott A, Salmonella Typhimurium ATCC 14028, and Sthapylococcus aureus ATCC 6538 was determined. Most of honeys analyzed showed values within quality parameters established by the Codex Alimentarius Commission in 2001. Eucalyptus flower honey and orange blossom honey showed the highest phenolic contents and antioxidant capacity. Bell flower, orange blossom, and eucalyptus flower honeys inhibited the growth of the 4 evaluated microorganisms. The remaining honeys affected at least 1 of the estimated growth parameters (increased lag phase, decreased growth rate, and/or maximum population density). Microorganism sensitivity to the antimicrobial activity of honeys followed the order B. cereus > L. monocytogenes > Salmonella Typhimurium > S. aureus. The monofloral honey samples from orange blossoms, and eucalyptus flowers demonstrated to be good sources of antioxidant and antimicrobial compounds. All the Mexican honey samples examined proved to be good sources of antioxidants and antimicrobial agents that might serve to maintain health and protect against several diseases. The results of the study showed that Mexican honeys display good quality parameters and antioxidant and antimicrobial activities. Mexican honey can be used as an additive in the food industry to increase the nutraceutical value of products. © 2011 Institute of Food Technologists®

Erosion and strength degradation of biomorphic SiC.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martinez-Fernandez, J.; de Arellano-Lopez, A. R; Varela-Feria, F. M.

2004-05-01

Solid-particle-erosion studies were conducted on biomorphic SiC based on eucalyptus and pine, reaction-bonded (RB) SiC, and hot-pressed (HP) SiC. The erodents were angular SiC abrasives of average diameter 63, 143, or 390 {mu}m and the impact velocity was 100 m s{sup -1}. Impact occurred at normal incidence. Material loss in all targets occurred by brittle fracture. The biomorphic specimens eroded by formation of both lateral and radial cracks and their erosion rates were higher than both conventional SiCs. The RB SiC eroded as a classic brittle material, by formation and propagation of lateral cracks. The HP SiC, the hardest target,more » was the most erosion resistant. In erosion of the HP SiC, the abrasive particles, especially the largest ones, fragmented upon impact. The resulting dissipation of energy led to relatively low erosion rates. Flexural strength before and after erosion was measured for the biomorphic eucalyptus, RB SiC, and HP SiC. Erosion damage reduced the flexural strengths of all of the specimens. The relative strength reductions were lowest for the biomorphic eucalyptus and highest for the HP SiC. The hot-pressed SiC responded as predicted by accepted models of impact damage in brittle solids. The responses of the biomorphic and reaction-bonded SiC specimens were modeled as if they consisted of only SiC and porosity. This approximation agreed reasonably well with observed degradations of strength.« less

Effect of nebulized eucalyptus on contamination of microbial plaque of endotracheal tube in ventilated patients

PubMed Central

Amini, Nazanin; Rezaei, Korosh; Yazdannik, Ahmadreza

2016-01-01

Background: Formation of biofilm and bacterial colonization within the endotracheal tube (ETT) are significant sources of airway contamination and play a role in the development of ventilator-associated pneumonia (VAP). This study was conducted to examine the effect of nebulized eucalyptus (NE) on bacterial colonization of ETT biofilm. Materials and Methods: We performed a randomized clinical trial in three intensive care units (ICUs) of an educational hospital. Seventy intubated patients were selected and randomly divided into intervention (n = 35) and control (n = 35) groups. The intervention group received 4 ml (5%) of eucalyptus in 6 ml normal saline every 8 h. The placebo group received only 10 ml of normal saline in the same way. On extubation, the interior of the tube was immediately sampled using a sterile swab for standard microbiological analysis. Chi-square and Fisher's exact tests were used for statistical analysis in SPSS. P values less than 0.05 were considered statistically significant. Results: In both samples, Klebsiella pneumoniae and Acinetobacter baumannii were the most frequently isolated bacteria. In the control group, heavy colonization was greater than in the intervention group (P = 0.002). The frequency of isolation of K. pneumoniae in the intervention group was lower than in the control group (P < 0.001). However, there was no difference between the two groups in other isolated bacteria. Conclusions: NE can reduce microbial contamination of the endotracheal tube biofilm in ventilated patients. Moreover, K. pneumoniae was the most sensitive to NE. PMID:27095990

Response of soil respiration and ecosystem carbon budget to vegetation removal in Eucalyptus plantations with contrasting ages

PubMed Central

Wu, Jianping; Liu, Zhanfeng; Huang, Guomin; Chen, Dima; Zhang, Weixin; Shao, Yuanhu; Wan, Songze; Fu, Shenglei

2014-01-01

Reforested plantations have substantial effects on terrestrial carbon cycling due to their large coverage area. Although understory plants are important components of reforested plantations, their effects on ecosystem carbon dynamics remain unclear. This study was designed to investigate the effects of vegetation removal/understory removal and tree girdling on soil respiration and ecosystem carbon dynamics in Eucalyptus plantations of South China with contrasting ages (2 and 24 years old). We conducted a field manipulation experiment from 2008 to 2009. Understory removal reduced soil respiration in both plantations, whereas tree girdling decreased soil respiration only in the 2-year-old plantations. The net ecosystem production was approximately three times greater in the 2-year-old plantations (13.4 t C ha−1 yr−1) than in the 24-year-old plantations (4.2 t C h−1 yr−1). The biomass increase of understory plants was 12.6 t ha−1 yr−1 in the 2-year-old plantations and 2.9 t ha−1 yr−1 in the 24-year-old plantations, accounting for 33.9% and 14.1% of the net primary production, respectively. Our findings confirm the ecological importance of understory plants in subtropical plantations based on the 2 years of data. These results also indicate that Eucalyptus plantations in China may be an important carbon sink due to the large plantation area. PMID:25179343

Effects of an inducible aiiA gene on disease resistance in Eucalyptus urophylla × Eucalyptus grandis.

PubMed

Ouyang, L J; Li, L M

2016-08-01

N-acyl-homoserine lactones (AHLs) are metabolites of mostly gram-negative bacteria and are critical signaling molecules in bacterial quorum-sensing systems. At threshold concentrations, AHLs can activate the expression of pathogenic genes and induce diseases. Therefore, reducing AHL concentrations is a key point of disease control in plants. AHL-lactonase, which is expressed by aiiA, is widespread in Bacillus sp and can hydrolyze AHLs. In the present study, we cloned aiiA from Bacillus subtilis by PCR. A plant expression vector of aiiA was constructed and name Pcam-PPP3-aiiA, in which expression of aiiA was controlled by the pathogen-inducible plant promoter PPP3. The recombinant plasmid was transferred into Eucalyptus × urophylla × E. grandis by an Agrobacterium-mediated transformation. PCR and Southern blotting showed that aiiA was successfully integrated into the E. urophylla × E. grandis genome and its expression was induced by Ralstonia solanacearum 12 h after inoculation, as shown by reverse transcription-PCR. The transcription efficacy of aiiA increased 43.88-, 30.65-, and 18.95-fold after inoculation with R. solanacearum, Erwinia carotovora ssp. zeae (Sabet) and Cylindrocladium quinqueseptatum, respectively as shown by RT-real-time PCR. Transgenic E.urophylla × E.grandis expressing the AIIA protein exhibited significantly enhanced disease resistance compared to non-transgenic plants by delaying the onset of wilting and reducing the disease index.

Overview in two parts: Right view showing orchard path on ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Overview in two parts: Right view showing orchard path on left eucalyptus windbreak bordering knoll on right. Camera facing 278" west. - Goerlitz House, 9893 Highland Avenue, Rancho Cucamonga, San Bernardino County, CA

Modeling of Carbon Sequestration on Eucalyptus Plantation in Brazililian Cerrado Region for Better Characterization of Net Primary Productivity

NASA Astrophysics Data System (ADS)

Echeverri, J. D.; Siqueira, M. B.

2013-05-01

Managed Forests have important roles in climate change due to their contribution to CO2 sequestration stored in their biomass, soils and products therefrom. Terrestrial net primary production (NPP, kgC/m2), equal to gross primary production minus autotrophic respiration, represents the carbon available for plant allocation to leaves, stems, roots, defensive compounds, and reproduction and is the basic measure of biological productivity. Tree growth, food production, fossil fuel production, and atmospheric CO2 levels are all strongly controlled by NPP. Accurate quantification of NPP at local to global scales is therefore central topic for carbon cycle researchers, foresters, land and resource managers, and politicians. For recent or current NPP estimates, satellite remote sensing can be used but for future climate scenarios, simulation models are required. There is an increasing trend to displace natural Brazilian Cerrado to Eucalyptus for paper mills and energy conversion from biomass. The objective of this research exercise is to characterize NPP from managed Eucalyptus plantation in the Brazilian Cerrado. The models selected for this study were the 3-PG and Biome-BGC. The selection of these models aims to cover a range of complexity that allow the evaluation of the processes modeled as to its relevance to a best estimate of productivity in eucalyptus forests. 3-PG model is the simplest of the models chosen for this exercise. Its main purpose is to estimate productivity of forests in timber production. The model uses the relationship of quantum efficiency in the transformation of light energy into biomass for vegetative growth calculations in steps in time of one month. Adverse weather conditions are treated with reduction factors applied in the top efficiency. The second model is the Biome-BGC that uses biology and geochemistry principles to estimate leaf-level photosynthesis based on limiting factors such as availability of light and nutrient constraints. The model does not consider any vertical structure, and the extrapolation of leaf scale is the scale of the ecosystem, which is accomplished by using leaf area index to variable on a temporal resolution of a day. Carbon allocation is computed by complex interactions between multiples carbon pools. Therefore the results obtained in modeling, it was possible to verify the applicability of the two models 3PG and Biome-BGC in estimate of NPP to eucalyptus energy forest in a Brazilian cerrado region, having a strong correlation to the sixth year of forest growth between the two models. The study also revealed that have input parameters in models that need to be measured with a good accuracy, because in function of these parameters, the NPP variation is very large. Finally the study revealed the importance of confronting the data obtained by 3PG and Biome-BGC with experimental data to improve performance modeled-based estimation.

Mosquito Bites

MedlinePlus

... spreading to the United States. One factor is climate change, which makes the conditions in some parts of ... United States. How can I prevent mosquito bites? Use an insect ... DEET, picaridin, IR3535, oil of lemon eucalyptus, or para-menthane-diol. It ...

Field evaluation of commercial repellents against the floodwater mosquito Psorophora columbiae (Diptera: Culicidae) in St. Johns County, Florida.

PubMed

Qualls, Whitney A; Xue, Rui-De; Holt, J Adam; Smith, Mike L; Moeller, Jeanne J

2011-11-01

Three plant-based repellents-REPEL LEMON Eucalyptus Insect Repellent Lotion (active ingredient [AI] 30% oil of eucalyptus), Bite Blocker Xtreme Sportsman Organic Insect Repellent ([AI] 3% soybean oil, 6% geranium oil, and 8% castor oil), and Bite Blocker BioUD Insect Repellent ([AI] 7.75% 2-undecanone)--were evaluated against OFF! ([AI] 15% N,N-diethyl-m-toluamide or N,N-diethyl-3-methyl-benzamide, also called DEET) at a field site in Elkton, FL, to determine the mean protection time provided against Psorophora columbiae (Dyar & Knab). These products provided different protection times against biting Ps. columbiae. REPEL provided the longest protection time (330 min) followed by Bite Blocker Xtreme Sportsman (163 min), Bite Blocker BioUD (140 min), and OFF! (130 min). This study provides the first information about plant-based insect repellent protection times against Ps. columbiae.

A multistressor, multitrait approach to assessing the effects of wind and dust on Eucalyptus tereticornis.

PubMed

Leonard, Ryan J; McArthur, Clare; Hochuli, Dieter F

2016-08-01

Plants are routinely subjected to multiple environmental stressors, and the ability to respond to these stressors determines species survival and ecological breadth. Despite stressors such as wind and dust significantly influencing plant development, morphology, and chemistry, the combined influence of these factors is yet to be investigated. We used a manipulative glasshouse approach to compare the morphological, physiological, and biomechanical responses of Eucalyptus tereticornis to the independent and combined effects of wind and dust. Wind decreased both E. tereticornis height and stem flexural stiffness. Additionally, wind had no effect on leaf physiology, nor did dust have any significant effect on any of the traits measured. Our results suggest that wind and dust in combination may have an additive effect on several plant traits and provide new insight into the effects and importance of studying wind, dust, and different stress combinations. © 2016 Botanical Society of America.

Effects of G, a Growth Regulator from Eucalyptus grandis, on Photosynthesis

PubMed Central

Sharkey, Thomas D.; Stevenson, Gay F.; Paton, Dugald M.

1982-01-01

A growth regulator (G; 4-ethyl-1-hydroxy-4,8,8,10,10 pentamethyl-7,9-dioxo-2,3 dioxyabicyclo (4.4.0) decene-5) from Eucalyptus grandis (Maiden) reduced stomatal conductance and also photosynthetic capacity when fed through the transpiration stream of detached leaves. The concentration of G required for this effect was high (10−4 molar), but the amount of G taken up (dose) was below the level which has previously been found in E. grandis leaves. Similar effects were observed in detached leaves of Xanthium strumarium L. though almost 10 times more G was required. G reduced CO2-dependent O2 evolution from isolated cells of X. strumarium. In spinach (Spinacia oleracea L.) chloroplasts, electron transport through photosystem II was reduced by G. It is proposed that G affects stomatal conductance and photosynthesis by reducing photosystem II activity in both the guard cell chloroplasts and mesophyll cell chloroplasts. PMID:16662322

Structural variation of eucalyptus lignin in a combination of hydrothermal and alkali treatments.

PubMed

Sun, Shao-Ni; Li, Han-Yin; Cao, Xue-Fei; Xu, Feng; Sun, Run-Cang

2015-01-01

In this work, the structural features of the lignin isolated with 2% NaOH at 90°C for 2.5h from the hydrothermally pretreated eucalyptus fibers at different temperatures (100-200°C) for different times (15-60min) were thoroughly investigated. Results showed that the hydrothermal pretreatment facilitated the separation of alkali lignin from the pretreated fibers. It was found that the linkages of β-O-4, β-β, and β-5 decreased gradually with the increase of hydrothermal severity. Furthermore, decreased molecular weights (1630-510g/mol), associated carbohydrates contents (1.99-0.05%) and aliphatic OH contents (3.37-0.65mmol/g), and increased phenolic OH contents (0.71-2.98mmol/g) and thermal stability of the alkali lignins were observed with the increase of the hydrothermal severity. Copyright © 2014 Elsevier Ltd. All rights reserved.

Natural gold particles in Eucalyptus leaves and their relevance to exploration for buried gold deposits

PubMed Central

Lintern, Melvyn; Anand, Ravi; Ryan, Chris; Paterson, David

2013-01-01

Eucalyptus trees may translocate Au from mineral deposits and support the use of vegetation (biogeochemical) sampling in mineral exploration, particularly where thick sediments dominate. However, biogeochemistry has not been routinely adopted partly because biotic mechanisms of Au migration are poorly understood. For example, although Au has been previously measured in plant samples, there has been doubt as to whether it was truly absorbed rather than merely adsorbed on the plant surface as aeolian contamination. Here we show the first evidence of particulate Au within natural specimens of living biological tissue (not from laboratory experimentation). This observation conclusively demonstrates active biogeochemical adsorption of Au and provides insight into its behaviour in natural samples. The confirmation of biogeochemical adsorption of Au, and of a link with abiotic processes, promotes confidence in an emerging technique that may lead to future exploration success and maintain continuity of supply. PMID:24149278

Pellets valorization of waste biomass harvested by coagulation of freshwater algae.

PubMed

Cancela, Ángeles; Sánchez, Ángel; Álvarez, Xana; Jiménez, Alejandro; Ortiz, Luis; Valero, Enrique; Varela, Paloma

2016-03-01

There is a comparison of different coagulants: calcium chloride (20, 60, 120 and 180 mg/L); sodium alginate (10 and 20 mg/L) and tannins of Eucalyptus globulus bark (10 and 20 mg/L) in order to make the most of each method. The results show that 20 mg/L of tannin achieved a recovery efficiency of 95.35±1.16, sodium alginate 90.49±0.53 and 84.04±2.29 for calcium chloride. Taking into account the economic side of the coagulants, obtaining tannins is a profitable process. Bark is waste biomass obtained in the forestry process; therefore it does not involve extra costs. Finally, the feasibility of making pellets from harvested algae was studied, and the results suggest that waste biomass pellets may be used as fuel in boilers in a mixture <54% with other waste sources as Eucalyptus g. branches. Copyright © 2016 Elsevier Ltd. All rights reserved.

Use of xylanase in the TCF bleaching of eucalyptus kraft pulp

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roncero, B.; Vidal, T.; Torres, A.L.

1996-10-01

Environmental pressures are forcing the pulp and paper industry to develop new technologies that reduce or eliminate the presence of various contaminants in bleaching plant effluents. Oxygen delignification techniques, replacement of elemental chlorine with chlorine dioxide, ozone, hydrogen peroxide and new agents as well as the use of xylanase enzymes for biobleaching, reduce o eliminate the production of chlorinated organic substances. This paper compares the sequence XOZP with OZP in the bleaching of Eucalyptus globulus kraft pulps. It has been studied the influence of enzymatic treatment on the consumption of bleaching agents: ozone and hydrogen peroxide. Chemical, physical, optical andmore » refining properties of pulps, as well as COD and colour of effluent are also studied. The xylanase treatment is positive and it is possible to manufacture fully bleached pulps at high brightness and viscosity without using chlorine compounds at a low ozone and hydrogen peroxide consumption.« less

Digestion and absorption ofEucalyptus essential oils in greater glider (Petauroide svolans) and brushtail possum (Trichosurus vulpecula).

PubMed

Foley, W J; Lassak, E V; Brophy, J

1987-11-01

Measurements were made of the quantity and composition of the steam-volatile essential oils in gastrointestinal tract contents of greater gliders fedEucalyptus radiata foliage and brushtail possums fedE. melliodora foliage. In both species, there was less oil in the stomach contents than in an equivalent mass of foliage. Only minor losses of leaf oils occurred during mastication by greater gliders, and absorption from the stomach appeared to be the major reason for the difference in the oil content of ingested leaves and of stomach contents. The apparent digestibility of oils over the whole gut was 96-97 %, although oils from the cecum and feces of both species contained compounds not present in the original leaf oils. Absorption of oils before they reach the hindgut should reduce the severity of antimicrobial effects but may involve a metabolic cost to the animal in detoxification and excretion.

Methanol production from Eucalyptus wood chips. Working Document 2. Vegetative propagation of Eucalypts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fishkind, H.H.

1982-04-01

The feasibility of large-scale plantation establishment by various methods was examined, and the following conclusions were reached: seedling plantations are limited in potential yield due to genetic variation among the planting stock and often inadequate supplies of appropriate seed; vegetative propagation by rooted cuttings can provide good genetic uniformity of select hybrid planting stock; however, large-scale production requires establishment and maintenance of extensive cutting orchards. The collection of shoots and preparation of cuttings, although successfully implemented in the Congo and Brazil, would not be economically feasible in Florida for large-scale plantations; tissue culture propagation of select hybrid eucalypts offers themore » only opportunity to produce the very large number of trees required to establish the energy plantation. The cost of tissue culture propagation, although higher than seedling production, is more than off-set by the increased productivity of vegetative plantations established from select hybrid Eucalyptus.« less

Bioactivity against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) of Cymbopogon citratus and Eucalyptus citriodora essential oils grown in Colombia.

PubMed

Olivero-Verbel, Jesús; Nerio, Luz S; Stashenko, Elena E

2010-06-01

Essential oils isolated from Cymbopogon citratus (DC) Stapf. and Eucalyptus citriodora Hook grown in Colombia were analysed by gas chromatography-mass spectrometry (GC-MS) and tested for repellent activity and contact toxicity against Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae). The main components of C. citratus oil were geranial (34.4%), neral (28.4%) and geraniol (11.5%), whereas those of E. citriodora were citronellal (40%), isopulegol (14.6%) and citronellol (13%). The mean repellent doses after 4 h exposure were 0.021 and 0.084 mL L(-1) for C. citratus and E. citriodora oils respectively-values lower than that observed for the commercial product IR3535 (0.686 mL L(-1)). These studies showed the composition and repellent activity of essential oils of C. citratus and E. citriodora, suggesting that these are potential candidates as insect repellents.

Antibacterial and antifungal activities of pyroligneous acid from wood of Eucalyptus urograndis and Mimosa tenuiflora.

PubMed

de Souza Araújo, E; Pimenta, A S; Feijó, F M C; Castro, R V O; Fasciotti, M; Monteiro, T V C; de Lima, K M G

2018-01-01

This work aimed to evaluate the antibacterial and antifungal activities of two types of pyroligneous acid (PA) obtained from slow pyrolysis of wood of Mimosa tenuiflora and of a hybrid of Eucalyptus urophylla × Eucalyptus grandis. Wood wedges were carbonized on a heating rate of 1·25°C min -1 until 450°C. Pyrolysis smoke was trapped and condensed to yield liquid products. Crude pyrolysis liquids were bidistilled under 5 mmHg vacuum yielding purified PA. Multi-antibiotic-resistant strains of Escherichia coli, Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) had their sensitivity to PA evaluated using agar diffusion test. Two yeasts were evaluated as well, Candida albicans (ATCC 10231) and Cryptococcus neoformans. GC-MS analysis of both PAs was carried out to obtain their chemical composition. Regression analysis was performed, and models were adjusted, with diameter of inhibition halos and PA concentration (100, 50 and 20%) as parameters. Identity of regression models and equality of parameters in polynomial orthogonal equations were verified. Inhibition halos were observed in the range 15-25 mm of diameter. All micro-organisms were inhibited by both types of PA even in the lowest concentration of 20%. The feasibility of the usage of PAs produced with wood species planted in large scale in Brazil was evident and the real potential as a basis to produce natural antibacterial and antifungal agents, with real possibility to be used in veterinary and zootechnical applications. © 2017 The Society for Applied Microbiology.

Resilience of Invaded Riparian Landscapes: The Potential Role of Soil-Stored Seed Banks

NASA Astrophysics Data System (ADS)

Tererai, Farai; Gaertner, Mirijam; Jacobs, Shayne M.; Richardson, David M.

2015-01-01

We investigated the potential role of soil-stored seed banks in driving vegetation recovery under varying intensities of invasion by the alien tree Eucalyptus camaldulensis along the Berg River in South Africa's Western Cape Province. We asked: How do richness, diversity, and composition of soil-stored seed banks vary with invasion intensity? What is the difference between the seed banks and above-ground vegetation with respect to species richness, diversity, composition, and structure? To what extent do soil-stored seed banks provide reliable sources for restoring native plant communities? Through a seedling-emergence approach, we compared seedling density, richness, and diversity in plots under varying Eucalyptus cover. Seed bank characteristics were also compared with those of the above-ground vegetation. Except in terms of diversity and density, the richness and composition of native species varied significantly among invasion conditions. Despite the paucity of native tree and shrub species in the seed bank, it was more diverse than extant vegetation. Some species occurred exclusively either in the seed bank or in the above-ground vegetation. Although this ecosystem has been degraded by several agents, including Eucalyptus invasion, soil-stored seed banks still offer modest potential for driving regeneration of native plant communities, but secondary invasions need to be managed carefully. Remnant populations of native plants in the above-ground vegetation remaining after E. camaldulensis clearing provide a more promising propagule source for rapid regeneration. Further work is needed to elucidate possible effects of invasion on successional pathways following E. camaldulensis removal and the effects of hydrochory on seed bank dynamics.

Characterization of Lignin Streams during Bionic Liquid-Based Pretreatment from Grass, Hardwood, and Softwood

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dutta, Tanmoy; Papa, Gabriella; Wang, Eileen

Delignification as a function of ionic liquid (IL) pretreatment has potential in terms of recovering and converting the fractionated lignin streams to renewable products. Renewable biogenic ionic liquids, or bionic liquids (eg. cholinium lysinate, ([Ch][Lys])), provide opportunities in terms of effective, economic and sustainable lignocellulosic biomass pretreatment. We have evaluated [Ch][Lys] pretreatment in terms of sugar and lignin yields for three different feedstocks: switchgrass, eucalyptus, and pine. Four lignin streams isolated during [Ch][Lys] pretreatment and enzymatic hydrolysis were comprehensively analyzed, tracking their changes in physical-chemical structures. We observed changes in major lignin linkages and lignin aromatics units (p-hydroxyphenyl (H), guaiacylmore » (G), and syringil (S)) that occurred during pretreatment. A compositional analysis of the different process streams and a comprehensive mass balance in conjunction with multiple analytical techniques (Nuclear Magnetic Resonance (NMR), Mass Spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), Gel Permeation Chromatography (GPC)) is presented. Qualitative and quantitative analyses indicates that there are significantly more lignin-carbohydrate interactions for G-rich lignin in pine. The lignin removal and extent of lignin depolymerization for switchgrass and eucalyptus were higher than pine, and follows the order of switchgrass > eucalyptus > pine. The recovered lignin from pretreated liquid contained a lower relative amount of carbohydrate signals than raw biomass, indicating a high degree of dissociation of lignin carbohydrate complex (LCC) linkages for all samples analyzed. The insights gained from this work contribute to better understanding of physiochemical properties of lignin streams generated during [Ch][Lys] pretreatment, offering a starting point for lignin valorization strategies.« less

Essential oil-loaded lipid nanoparticles for wound healing.

PubMed

Saporito, Francesca; Sandri, Giuseppina; Bonferoni, Maria Cristina; Rossi, Silvia; Boselli, Cinzia; Icaro Cornaglia, Antonia; Mannucci, Barbara; Grisoli, Pietro; Vigani, Barbara; Ferrari, Franca

2018-01-01

Chronic wounds and severe burns are diseases responsible for severe morbidity and even death. Wound repair is a crucial process and tissue regeneration enhancement and infection prevention are key factors to minimize pain, discomfort, and scar formation. The aim of this work was the development of lipid nanoparticles (solid lipid nanoparticles and nanostructured lipid carriers [NLC]), to be loaded with eucalyptus or rosemary essential oils and to be used, as medical devices, to enhance healing of skin wounds. Lipid nanoparticles were based on natural lipids: cocoa butter, as solid lipid, and olive oil or sesame oil, as liquid lipids. Lecithin was chosen as surfactant to stabilize nanoparticles and to prevent their aggregation. The systems were prepared by high shear homogenization followed by ultrasound application. Nanoparticles were characterized for physical-chemical properties, bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward normal human dermal fibroblasts. Antimicrobial activity of nanoparticles was evaluated against two reference microbial strains, one of Staphylococcus aureus , the other of Streptococcus pyogenes . Finally, the capability of nanoparticles to promote wound healing in vivo was evaluated on a rat burn model. NLC based on olive oil and loaded with eucalyptus oil showed appropriate physical-chemical properties, good bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward fibroblasts, associated to antimicrobial properties. Moreover, the in vivo results evidenced the capability of these NLC to enhance the healing process. Olive oil, which is characterized by a high content of oleic acid, proved to exert a synergic effect with eucalyptus oil with respect to antimicrobial activity and wound repair promotion.

Species richness in natural and disturbed habitats: Asteraceae and Flower-head insects (Tephritidae: Diptera).

PubMed

Diniz, Soraia; Prado, Paulo I; Lewinsohn, Thomas M

2010-01-01

Anthropogenic changes in the landscape result in an environmental mosaic with serious consequences for biodiversity. The aim of the present study was to assess the effects of the anthropogenic changes on Asteraceae richness and abundance, and to evaluate the consequences for the richness of Tephritidae assemblages in five sampling sites, with three sampled habitats in each: cerrado (Brazilian savanna), eucalyptus stands and pasture. Sampling was carried out in 15 random transects (cerrados and one pasture) and in 30 transects (eucalyptus stands and the remaining pastures). Composition, species richness and insect abundance in each habitat type was estimated by sampling the flower heads for each species of host plant, collected by four people for 1h. Differences in mean abundance of plant population between habitats and sites were tested by two-way ANOVA. Differences in plant species richness between habitats and sites and effects of habitat, site and host plant richness on insect richness were tested using a generalized linear model with Poisson errors. Within each sampling site, cerrados showed higher species richness of Asteraceae than pastures and eucalyptus stands. There were also significant differences in plant richness among sites. Mean population abundance values were significantly different among habitats, but not among sites. Increased host plant richness led to significant insect species richness. There were no additional significant effects of habitat on insect richness. Therefore, anthropogenic alterations in landscape determined the impoverishment of plant assemblages and therefore of insect assemblages, because of the positive relationship between host plant richness and insect richness.

Repellent and Larvicidal Activity of the Essential Oil From Eucalyptus nitens Against Aedes aegypti and Aedes albopictus (Diptera: Culicidae).

PubMed

Alvarez Costa, Agustín; Naspi, Cecilia V; Lucia, Alejandro; Masuh, Héctor M

2017-05-01

Dengue, chikungunya, and yellow fever are important vector-borne diseases transmitted by female mosquitoes when they feed on humans. The use of repellents based on natural products is an alternative for personal protection against these diseases. Application of chemicals with larvicidal activity is another strategy for controlling the mosquito population. The repellent and larvicidal activities of the essential oil from Eucalyptus nitens were tested against Aedes aegypti and Aedes albopictus, the main vectors of these arboviruses. The essential oil was extracted by hydrodistillation and then analyzed by gas chromatography-mass spectrometry. The main components of Eucalyptus nitens essential oil were found to be terpenes such as 1,8-cineole and p-cymene, followed by β-triketones and alkyl esters. The repellent activity of the essential oil against both species was significantly higher when compared with the main component, 1,8-cineole, alone. These results indicate that the repellent effect of E. nitens is not due only to the main component, 1,8-cineole, but also that other compounds may be responsible. Aedes aegypti was found to be more tolerant to the essential oil larvicidal effects than Ae. albopictus (Ae. aegypti LC50 = 52.83 ppm, Ae. albopictus LC 50 = 28.19 ppm). The repellent and larvicidal activity could be associated to the presence of cyclic β-triketones such as flavesone, leptospermone, and isoleptospermone. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Commercial tree species discrimination using airborne AISA Eagle hyperspectral imagery and partial least squares discriminant analysis (PLS-DA) in KwaZulu-Natal, South Africa

NASA Astrophysics Data System (ADS)

Peerbhay, Kabir Yunus; Mutanga, Onisimo; Ismail, Riyad

2013-05-01

Discriminating commercial tree species using hyperspectral remote sensing techniques is critical in monitoring the spatial distributions and compositions of commercial forests. However, issues related to data dimensionality and multicollinearity limit the successful application of the technology. The aim of this study was to examine the utility of the partial least squares discriminant analysis (PLS-DA) technique in accurately classifying six exotic commercial forest species (Eucalyptus grandis, Eucalyptus nitens, Eucalyptus smithii, Pinus patula, Pinus elliotii and Acacia mearnsii) using airborne AISA Eagle hyperspectral imagery (393-900 nm). Additionally, the variable importance in the projection (VIP) method was used to identify subsets of bands that could successfully discriminate the forest species. Results indicated that the PLS-DA model that used all the AISA Eagle bands (n = 230) produced an overall accuracy of 80.61% and a kappa value of 0.77, with user's and producer's accuracies ranging from 50% to 100%. In comparison, incorporating the optimal subset of VIP selected wavebands (n = 78) in the PLS-DA model resulted in an improved overall accuracy of 88.78% and a kappa value of 0.87, with user's and producer's accuracies ranging from 70% to 100%. Bands located predominantly within the visible region of the electromagnetic spectrum (393-723 nm) showed the most capability in terms of discriminating between the six commercial forest species. Overall, the research has demonstrated the potential of using PLS-DA for reducing the dimensionality of hyperspectral datasets as well as determining the optimal subset of bands to produce the highest classification accuracies.

Isolation of intact sub-dermal secretory cavities from Eucalyptus

PubMed Central

2010-01-01

Background The biosynthesis of plant natural products in sub-dermal secretory cavities is poorly understood at the molecular level, largely due to the difficulty of physically isolating these structures for study. Our aim was to develop a protocol for isolating live and intact sub-dermal secretory cavities, and to do this, we used leaves from three species of Eucalyptus with cavities that are relatively large and rich in essential oils. Results Leaves were digested using a variety of commercially available enzymes. A pectinase from Aspergillus niger was found to allow isolation of intact cavities after a relatively short incubation (12 h), with no visible artifacts from digestion and no loss of cellular integrity or cavity contents. Several measurements indicated the potential of the isolated cavities for further functional studies. First, the cavities were found to consume oxygen at a rate that is comparable to that estimated from leaf respiratory rates. Second, mRNA was extracted from cavities, and it was used to amplify a cDNA fragment with high similarity to that of a monoterpene synthase. Third, the contents of the cavity lumen were extracted, showing an unexpectedly low abundance of volatile essential oils and a sizeable amount of non-volatile material, which is contrary to the widely accepted role of secretory cavities as predominantly essential oil repositories. Conclusions The protocol described herein is likely to be adaptable to a range of Eucalyptus species with sub-dermal secretory cavities, and should find wide application in studies of the developmental and functional biology of these structures, and the biosynthesis of the plant natural products they contain. PMID:20807444

ETHNOBOTANICAL STUDY OF PLANTS USED TO TREAT ASTHMA IN THE MARITIME REGION IN TOGO

PubMed Central

Gbekley, Holaly E.; Katawa, Gnatoulma; Karou, Simplice D.; Anani, SKokou; Tchadjobo, Tchacondo; Ameyapoh, Yaovi; Batawila, Komlan; Simpore, Jacques

2017-01-01

Background: Asthma is one of the most common chronic diseases in modern society and it is evident that its incidence and severity are increasing, however very little is known about the plants used in the management of the disease. This study therefore aimed to document the plants usage in the Togolese traditional medicine to treat asthma. Methodology: From January to June 2015, an ethnobotanical survey was conducted using a semi-structured questionnaire with traditional healers (THs) in the southern region of Togo. The importance of the plants species was assessed by the calculated use values. Results: In Total, 121 THs (92 males and 29 females) were interviewed and 98 plants species belonging to 54 families were identified as curing asthma. The most represented families were: Leguminosae with 7 species followed by Euphorbiaceae and Rutaceae contributing with 6 and 5 species respectively. Based on the calculated use values the most important species were Carcica papaya L., Cataranthus roseus L., Eucalyptus camaldulensis Dehnh., Piper guineense Thonn., Eucalyptus citriodora Hook., Eucalyptus globules Labill. and Euphorbia hirta L. The leaves and the root were the parts predominantly used to prepare the formulations, mainly decoctions, administrated by oral route. Clinical manifestations such as wheezing (91.74%), difficulty as speaking or coughing (73.55%), dyspnea (66.94%), dry cough (52.89%), sweating and increased heart rate (52.07%) were used by TH to diagnose the disease. Conclusion: This study showed initial evidence of the use of plant materials by Togolese TH to heal asthma. These results could be a starting point for laboratory screenings. PMID:28480398

Water and vegetation indices by using MODIS products for eucalyptus, pasture, and natural ecosystems in the eastern São Paulo state, Southeast Brazil

NASA Astrophysics Data System (ADS)

de C. Teixeira, Antônio H.; Leivas, Janice F.; Ronquim, Carlos C.; Garçon, Edlene A. M.; Bayma-Silva, Gustavo

2017-10-01

Eucalyptus (Ec) and pasture (Pt) are expanding while natural vegetation (Nv) are losing space in the Paraíba Valley, eastern side of the São Paulo state, Southeast Brazil. For quantification of water and vegetation conditions, the MODIS product MOD13Q1 was used together with a net of weather stations and vegetation land masks during the year 2015. The SAFER algorithm was applied to retrieve the actual evapotranspiration (ET), which was combined with the Monteith's radiation use efficiency (RUE) model to estimate the biomass production (BIO). Three moisture indices were applied, the climatic water balance ratio (WBr), the ratio of precipitation (P) to ET, the water balance deficit (WBd), the difference between P and ET, and the evapotranspiration ratio (ETr), the ratio of ET to the reference evapotranspiration (ET0). On the one hand, the highest ET rates for the Ec ecosystem should be a negative aspect under water scarcity conditions; however, it presented the best water productivity. Although the Ec ecosystem presenting the lowest WBr and WBd values, it had the highest ETr, averaging 0.92, when comparing to those for Nv (0.88) and Pt (0.79). These results indicated that eucalyptus plants have greater ability of conserving soil moisture in their root zones, increasing WP, when comparing with Pt and Nv ecosystems. These water relationships are relevant issues under the land-use change conditions in the Paraiba Valley, confirming the suitability of using the MODIS products together with weather stations to study the ecosystem dynamics.

Essential oil-loaded lipid nanoparticles for wound healing

PubMed Central

Saporito, Francesca; Sandri, Giuseppina; Bonferoni, Maria Cristina; Rossi, Silvia; Boselli, Cinzia; Icaro Cornaglia, Antonia; Mannucci, Barbara; Grisoli, Pietro; Vigani, Barbara; Ferrari, Franca

2018-01-01

Chronic wounds and severe burns are diseases responsible for severe morbidity and even death. Wound repair is a crucial process and tissue regeneration enhancement and infection prevention are key factors to minimize pain, discomfort, and scar formation. The aim of this work was the development of lipid nanoparticles (solid lipid nanoparticles and nanostructured lipid carriers [NLC]), to be loaded with eucalyptus or rosemary essential oils and to be used, as medical devices, to enhance healing of skin wounds. Lipid nanoparticles were based on natural lipids: cocoa butter, as solid lipid, and olive oil or sesame oil, as liquid lipids. Lecithin was chosen as surfactant to stabilize nanoparticles and to prevent their aggregation. The systems were prepared by high shear homogenization followed by ultrasound application. Nanoparticles were characterized for physical–chemical properties, bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward normal human dermal fibroblasts. Antimicrobial activity of nanoparticles was evaluated against two reference microbial strains, one of Staphylococcus aureus, the other of Streptococcus pyogenes. Finally, the capability of nanoparticles to promote wound healing in vivo was evaluated on a rat burn model. NLC based on olive oil and loaded with eucalyptus oil showed appropriate physical–chemical properties, good bioadhesion, cytocompatibility, in vitro proliferation enhancement, and wound healing properties toward fibroblasts, associated to antimicrobial properties. Moreover, the in vivo results evidenced the capability of these NLC to enhance the healing process. Olive oil, which is characterized by a high content of oleic acid, proved to exert a synergic effect with eucalyptus oil with respect to antimicrobial activity and wound repair promotion. PMID:29343956

Species climate range influences hydraulic and stomatal traits in Eucalyptus species.

PubMed

Bourne, Aimee E; Creek, Danielle; Peters, Jennifer M R; Ellsworth, David S; Choat, Brendan

2017-07-01

Plant hydraulic traits influence the capacity of species to grow and survive in water-limited environments, but their comparative study at a common site has been limited. The primary aim of this study was to determine whether selective pressures on species originating in drought-prone environments constrain hydraulic traits among related species grown under common conditions. Leaf tissue water relations, xylem anatomy, stomatal behaviour and vulnerability to drought-induced embolism were measured on six Eucalyptus species growing in a common garden to determine whether these traits were related to current species climate range and to understand linkages between the traits. Hydraulically weighted xylem vessel diameter, leaf turgor loss point, the water potential at stomatal closure and vulnerability to drought-induced embolism were significantly ( P < 0·05) correlated with climate parameters from the species range. There was a co-ordination between stem and leaf parameters with the water potential at turgor loss, 12 % loss of conductivity and the point of stomatal closure significantly correlated. The correlation of hydraulic, stomatal and anatomical traits with climate variables from the species' original ranges suggests that these traits are genetically constrained. The conservative nature of xylem traits in Eucalyptus trees has important implications for the limits of species responses to changing environmental conditions and thus for species survival and distribution into the future, and yields new information for physiological models. © The Author 2017. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com

Water stress and recovery in the performance of two Eucalyptus globulus clones: physiological and biochemical profiles.

PubMed

Correia, Barbara; Pintó-Marijuan, Marta; Neves, Lucinda; Brossa, Ricard; Dias, Maria Celeste; Costa, Armando; Castro, Bruno B; Araújo, Clara; Santos, Conceição; Chaves, Maria Manuela; Pinto, Glória

2014-04-01

Eucalyptus plantations are among the most productive forest stands in Portugal and Spain, being mostly used for pulp production and, more recently, as an energy crop. However, the region's Mediterranean climate, with characteristic severe summer drought, negatively affects eucalypt growth and increases mortality. Although the physiological response to water shortage is well characterized for this species, evidence about the plants' recovery ability remains scarce. In order to assess the physiological and biochemical response of Eucalyptus globulus during the recovery phase, two genotypes (AL-18 and AL-10) were submitted to a 3-week water stress period at two different intensities (18 and 25% of field capacity), followed by 1 week of rewatering. Recovery was assessed 1 day and 1 week after rehydration. Drought reduced height, biomass, water potential, NPQ and gas exchange in both genotypes. Contrarily, the levels of pigments, chlorophyll fluorescence parameters (F(v) /F(m) and (φPSII)), MDA and ABA increased. During recovery, the physiological and biochemical profile of stressed plants showed a similar trend: they experienced reversion of altered traits (MDA, ABA, E, g(s), pigments), while other parameters did not recover ((φPSII), NPQ). Furthermore, an overcompensation of CO(2) assimilation was achieved 1 week after rehydration, which was accompanied by greater growth and re-establishment of oxidative balance. Both genotypes were tolerant to the tested conditions, although clonal differences were found. AL-10 was more productive and showed a more rapid and dynamic response to rehydration (namely in carotenoid content, (φPSII) and NPQ) compared to clone AL-18. © 2013 Scandinavian Plant Physiology Society.

Assessment of penetration potential of pH responsive double walled biodegradable nanogels coated with eucalyptus oil for the controlled delivery of 5-fluorouracil: In vitro and ex vivo studies.

PubMed

Sahu, Prashant; Kashaw, Sushil K; Jain, Sanyog; Sau, Samaresh; Iyer, Arun K

2017-05-10

Penetration enhancers coated biodegradable polymeric nanogels loaded with cytotoxic drugs applied via the topical route, can be a promising strategy for improving the chemotherapeutic efficiency of skin cancers. The major objective of proposed research was to investigate the in vitro and ex vivo chemotherapeutic potential of double walled PLGA-chitosan biodegradable nanogel entrapped with 5-fluororuacil (5-FU) coated with eucalyptus oil, topically applied onto the skin. 5-FU was first entrapped in PLGA core by solvent evaporation technique followed by coating with cationic chitosan for ionic interaction with anionic skin cancer cell membrane. A surface coating of eucalyptus oil (1%) was employed to improve the penetration efficacy of the nanogel into stratum corneum. The surface modified biodegradable double walled nanogel was characterized for particle size, charge and thermal properties followed by pH dependent in vitro analysis. Human keratinocyte (HaCaT) cell line was employed for the bio- and cyto-compatibility testing prior to the hemolysis assay and coagulation assessment. A porcine skin ex vivo screening was performed for assessing the penetration potential of the nanogels. DLS and TEM revealed a particle size about 170nm for the double walled nanogels. The nanogels also exhibited high thermal stability as analyzed by thermogravimetry (TG) and differential thermal analysis (DTA). The drug entrapment efficacy was about ~40%. The drug release showed sustained release pattern noted up to 24h. The low hemolysis of 2.39% with short prothrombin time (PT) and activated partial thromboplastin time (APTT) of 14.2 and 35.5s respectively, revealed high biocompatibility of the nanogels. The cellular uptake and localization was assessed by confocal microscopy. The cytotoxicity (MTT assay) on HaCaT cell line demonstrated high cytocompatibilty of the nanogels. An ex vivo evaluation using porcine skin displayed efficient and steady state flux of 5-FU from the biodegradable nanogles into the skin, while the histology of the porcine skin revealed enhanced penetration potential of eucalyptus oil coated PLGA-chitosan double walled nanogels. Taken together the in vivo and ex vivo results portend promising potential for the utility of the biodegradable nanogels for treating skin cancers. Copyright © 2017. Published by Elsevier B.V.

Insect herbivory in a mature Eucalyptus woodland canopy depends on leaf phenology but not CO2 enrichment.

PubMed

Gherlenda, Andrew N; Moore, Ben D; Haigh, Anthony M; Johnson, Scott N; Riegler, Markus

2016-10-19

Climate change factors such as elevated atmospheric carbon dioxide concentrations (e[CO 2 ]) and altered rainfall patterns can alter leaf composition and phenology. This may subsequently impact insect herbivory. In sclerophyllous forests insects have developed strategies, such as preferentially feeding on new leaf growth, to overcome physical or foliar nitrogen constraints, and this may shift under climate change. Few studies of insect herbivory at elevated [CO 2 ] have occurred under field conditions and none on mature evergreen trees in a naturally established forest, yet estimates for leaf area loss due to herbivory are required in order to allow accurate predictions of plant productivity in future climates. Here, we assessed herbivory in the upper canopy of mature Eucalyptus tereticornis trees at the nutrient-limited Eucalyptus free-air CO 2 enrichment (EucFACE) experiment during the first 19 months of CO 2 enrichment. The assessment of herbivory extended over two consecutive spring-summer periods, with a first survey during four months of the [CO 2 ] ramp-up phase after which full [CO 2 ] operation was maintained, followed by a second survey period from months 13 to 19. Throughout the first 2 years of EucFACE, young, expanding leaves sustained significantly greater damage from insect herbivory (between 25 and 32 % leaf area loss) compared to old or fully expanded leaves (less than 2 % leaf area loss). This preference of insect herbivores for young expanding leaves combined with discontinuous production of new foliage, which occurred in response to rainfall, resulted in monthly variations in leaf herbivory. In contrast to the significant effects of rainfall-driven leaf phenology, elevated [CO 2 ] had no effect on leaf consumption or preference of insect herbivores for different leaf age classes. In the studied nutrient-limited natural Eucalyptus woodland, herbivory contributes to a significant loss of young foliage. Leaf phenology is a significant factor that determines the level of herbivory experienced in this evergreen sclerophyllous woodland system, and may therefore also influence the population dynamics of insect herbivores. Furthermore, leaf phenology appears more strongly impacted by rainfall patterns than by e[CO 2 ]. e[CO 2 ] responses of herbivores on mature trees may only become apparent after extensive CO 2 fumigation periods.

Biomass Supply Chain and Conversion Economics of Cellulosic Ethanol

NASA Astrophysics Data System (ADS)

Gonzalez, Ronalds W.

2011-12-01

Cellulosic biomass is a potential and competitive source for bioenergy production, reasons for such acclamation include: biomass is one the few energy sources that can actually be utilized to produce several types of energy (motor fuel, electricity, heat) and cellulosic biomass is renewable and relatively found everywhere. Despite these positive advantages, issues regarding cellulosic biomass availability, supply chain, conversion process and economics need a more comprehensive understanding in order to identify the near short term routes in biomass to bioenergy production. Cellulosic biomass accounts for around 35% to 45% of cost share in cellulosic ethanol production, in addition, different feedstock have very different production rate, (dry ton/acre/year), availability across the year, and chemical composition that affect process yield and conversion costs as well. In the other hand, existing and brand new conversion technologies for cellulosic ethanol production offer different advantages, risks and financial returns. Ethanol yield, financial returns, delivered cost and supply chain logistic for combinations of feedstock and conversion technology are investigated in six studies. In the first study, biomass productivity, supply chain and delivered cost of fast growing Eucalyptus is simulated in economic and supply chain models to supply a hypothetic ethanol biorefinery. Finding suggests that Eucalyptus can be a potential hardwood grown specifically for energy. Delivered cost is highly sensitive to biomass productivity, percentage of covered area. Evaluated at different financial expectations, delivered cost can be competitive compared to current forest feedstock supply. In the second study, Eucalyptus biomass conversion into cellulosic ethanol is simulated in the dilute acid pretreatment, analysis of conversion costs, cost share, CAPEX and ethanol yield are examined. In the third study, biomass supply and delivered cost of loblolly pine is simulated in economic and supply chain models specifically for biomass to bioenergy production. The study suggest that this species can be profitably managed for biomass production with rotation length of 11 to 12 years and with a stand tree density of 1,200 trees per acre. Optimum rotation length is greatly affected by seedlings costs and biomass productivity. In the fourth study, a evaluation of seven different feedstocks (loblolly pine, natural mixed hardwood, Eucalyptus, switchgrass, miscanthus, corn stover and sweet sorghum) is made in terms of supply chain, biomass delivered costs, dollar per ton of carbohydrate and dollar per million BTU delivered to a biorefinery. Forest feedstocks present better advantages in terms of a well established supply chain, year round supply and no need for biomass storage. In the same context biomass delivered costs, as well as cost to delivered one ton of carbohydrate and one million BTU is lower in forest feedstocks. In the fifth study, conversion costs, profitability and sensitivity analysis for a novel pretreatment process, green liquor, are modeled for ethanol production with loblolly pine, natural mixed hardwood and Eucalyptus as feedstocks, evaluated in two investment scenarios: green field and repurposing of an old kraft pulp mill. Better financial returns are perceived in the natural hardwood - repurposing scenario, mainly due to lower CAPEX and lower enzyme charge and cost. In the sixth study, conversion cost, CAPEX, ethanol yield and profitability for the thermochemical process (indirect gasification and production of mixed alcohol) is simulated for loblolly pine, natural hardwood, eucalyptus, corn stover and switchgrass. Higher ethanol yield with forest feedstock (due to higher content of %C and %H) result in better economic performance, when compare to agriculture biomass. This research indicates that forest feedstock outperform agriculture biomass in terms of delivered costs, supply chain, ethanol yield and process profitability. Loblolly pine seems to be more suitable for thermochemical processes, while hardwood suit better for biochemical conversion (based on the technologies studied).

Emergy and Eco-exergy Evaluation of Four Forest Restoration Modes

EPA Science Inventory

Four different forest restoration modes (Acacia mangium plantation, mixed-native species plantation, conifer plantation and Eucalyptus plantation) were evaluated using Energy System Theory and the emergy synthesis method. In addition, the eco-exergies of the four forest restorati...

Medium density fiberboard made from Eucalyptus saligna

Treesearch

Andrzej M. Krzysik; James H. Muehl; John A. Youngquist; Fabio Spina Franca

2001-01-01

The production of industrial wood from natural forests is predicted to decline in the future. Factors that will contribute to this decline include changes in land use patterns, depletion of resources in some parts of the world, and the withdrawal of...

The potential of energy farming in the southeastern California desert

NASA Astrophysics Data System (ADS)

Lew, V.

1980-04-01

The use of energy forms to provide future sources of energy for California is considered. Marginal desert lands in southeastern California are proposed for the siting of energy farms using acacia, eucalyptus, euphorbia, guayule, jojoba, mesquite, or tamarisk.

Proceedings of the eighteenth southern forest tree improvement conference

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1985-01-01

This book contains 50 papers. Some of the titles are: Genetic Engineering in Forest Trees; Biotechnology and Forest Genetics: An Industry Perspective; Biomass Characteristics of Sycamore Coppice Influenced by Parentage and Type of Plant Stock; and Micropropagation of Eucalyptus viminalis.

3. EASTERN EDGE OF POST ENGINEER'S SHOPS AND YARD, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. EASTERN EDGE OF POST ENGINEER'S SHOPS AND YARD, LOOKING 312 DEGREES NORTH WEST, EUCALYPTUS TREES DENOTE EDGE OF PRESIDIO. - Presidio of San Francisco, Post Engineer's Headquarters Office, Crissy Field North cantonment, San Francisco, San Francisco County, CA

Equations for predicting biomass of six introduced tree species, island of Hawaii

Treesearch

Thomas H. Schukrt; Robert F. Strand; Thomas G. Cole; Katharine E. McDuffie

1988-01-01

Regression equations to predict total and stem-only above-ground dry biomass for six species (Acacia melanoxylon, Albizio falcataria, Eucalyptus globulus, E. grandis, E. robusta, and E. urophylla) were developed by felling and measuring 2- to 6-year-old...

77 FR 7123 - ArborGen, LLC; Availability of an Environmental Assessment for Controlled Release of a...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-10

... Eucalyptus. ArborGen is requesting that trees be allowed to flower at four locations in Alabama, Florida and... have indicated they will not allow these trees to flower at these locations. Permit application 11-052...

Cryopreservation of eucalyptus genetic resources

USDA-ARS?s Scientific Manuscript database

The long-term preservation of forest genetic resources is a vital part of preserving our forest crops for future generations. Unfortunately there are few genebanks dedicated to forest trees and very few methods aside from field plantings of germplasm collections of elite clonal individuals. The use ...

Growth and yield of some Eucalypts of interest to California

Treesearch

Roger G. Skolmen

1983-01-01

Eucalyptus globulus ssp. globulus, E. viminalis, E. grandis, and E. camaldulensis were selected as the species of interest in California. Yield data for these species from around the world indicate that wide variation can be expected among species, provenances, sites, and...

Environmental Conservation through the People's Movements in India.

ERIC Educational Resources Information Center

Asopa, Sheel K.

1993-01-01

Describes three popular environmental campaigns against deforestation in India. Discusses the folk dimension, mass mobilization, and geographic extent of the Chipko Movement; the movement against a government program to plant eucalyptus trees; and the movement to save villages in government-labeled "wastelands." (MDH)

Fungal Planet description sheets: 371-399.

PubMed

Crous, P W; Wingfield, M J; Le Roux, J J; Richardson, D M; Strasberg, D; Shivas, R G; Alvarado, P; Edwards, J; Moreno, G; Sharma, R; Sonawane, M S; Tan, Y P; Altés, A; Barasubiye, T; Barnes, C W; Blanchette, R A; Boertmann, D; Bogo, A; Carlavilla, J R; Cheewangkoon, R; Daniel, R; de Beer, Z W; de Jesús Yáñez-Morales, M; Duong, T A; Fernández-Vicente, J; Geering, A D W; Guest, D I; Held, B W; Heykoop, M; Hubka, V; Ismail, A M; Kajale, S C; Khemmuk, W; Kolařík, M; Kurli, R; Lebeuf, R; Lévesque, C A; Lombard, L; Magista, D; Manjón, J L; Marincowitz, S; Mohedano, J M; Nováková, A; Oberlies, N H; Otto, E C; Paguigan, N D; Pascoe, I G; Pérez-Butrón, J L; Perrone, G; Rahi, P; Raja, H A; Rintoul, T; Sanhueza, R M V; Scarlett, K; Shouche, Y S; Shuttleworth, L A; Taylor, P W J; Thorn, R G; Vawdrey, L L; Solano-Vidal, R; Voitk, A; Wong, P T W; Wood, A R; Zamora, J C; Groenewald, J Z

2015-12-01

Novel species of fungi described in the present study include the following from Australia: Neoseptorioides eucalypti gen. & sp. nov. from Eucalyptus radiata leaves, Phytophthora gondwanensis from soil, Diaporthe tulliensis from rotted stem ends of Theobroma cacao fruit, Diaporthe vawdreyi from fruit rot of Psidium guajava, Magnaporthiopsis agrostidis from rotted roots of Agrostis stolonifera and Semifissispora natalis from Eucalyptus leaf litter. Furthermore, Neopestalotiopsis egyptiaca is described from Mangifera indica leaves (Egypt), Roussoella mexicana from Coffea arabica leaves (Mexico), Calonectria monticola from soil (Thailand), Hygrocybe jackmanii from littoral sand dunes (Canada), Lindgomyces madisonensis from submerged decorticated wood (USA), Neofabraea brasiliensis from Malus domestica (Brazil), Geastrum diosiae from litter (Argentina), Ganoderma wiiroense on angiosperms (Ghana), Arthrinium gutiae from the gut of a grasshopper (India), Pyrenochaeta telephoni from the screen of a mobile phone (India) and Xenoleptographium phialoconidium gen. & sp. nov. on exposed xylem tissues of Gmelina arborea (Indonesia). Several novelties are introduced from Spain, namely Psathyrella complutensis on loamy soil, Chlorophyllum lusitanicum on nitrified grasslands (incl. Chlorophyllum arizonicum comb. nov.), Aspergillus citocrescens from cave sediment and Lotinia verna gen. & sp. nov. from muddy soil. Novel foliicolous taxa from South Africa include Phyllosticta carissicola from Carissa macrocarpa, Pseudopyricularia hagahagae from Cyperaceae and Zeloasperisporium searsiae from Searsia chirindensis. Furthermore, Neophaeococcomyces is introduced as a novel genus, with two new combinations, N. aloes and N. catenatus. Several foliicolous novelties are recorded from La Réunion, France, namely Ochroconis pandanicola from Pandanus utilis, Neosulcatispora agaves gen. & sp. nov. from Agave vera-cruz, Pilidium eucalyptorum from Eucalyptus robusta, Strelitziana syzygii from Syzygium jambos (incl. Strelitzianaceae fam. nov.) and Pseudobeltrania ocoteae from Ocotea obtusata (Beltraniaceae emend.). Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa.

Effects of cadmium and resource quality on freshwater detritus processing chains: a microcosm approach with two insect species.

PubMed

Campos, Diana; Alves, Artur; Lemos, Marco F L; Correia, António; Soares, Amadeu M V M; Pestana, João L T

2014-07-01

Detritus processing is vital for freshwater ecosystems that depend on the leaf litter from riparian vegetation and is mediated by microorganisms and aquatic invertebrates. Shredder invertebrates transform coarse particulate organic matter into fine particulate organic matter used as food by collector species. Direct and indirect effects of contaminants can impair detritus processing and thus affect the functioning of these ecosystems. Here, we assessed the combined effects of a toxic metal (cadmium) and resource quality (leaf species) on detritus processing and shredder-collector interactions. We considered two types of leaves, alder and eucalyptus that were microbially conditioned under different Cd concentrations in the laboratory. The microbial communities present on leaves were analyzed by Denaturing Gradient Gel Electrophoresis (DGGE), and we also measured microbial respiration rates. Sericostoma vittatum (a caddisfly shredder) and Chironomus riparius (a midge collector) were also exposed to Cd and allowed to consume the corresponding alder or eucalyptus leaves. We evaluated C. riparius growth and leaf mass loss in multispecies microcosms. Cadmium exposure affected leaf conditioning and fungal diversity on both leaf species, as assessed by DGGE. Cadmium exposure also affected the mass loss of alder leaves by reductions in detritivore feeding, and impaired C. riparius growth. Chironomus riparius consumed alder leaf discs in the absence of shredders, but S. vittatum appear to promote C. riparius growth in treatments containing eucalyptus. These results show that indirect effects of contaminants along detritus-processing chains can occur through effects on shredder-collector interactions such as facilitation but they also depend on the nutritional quality of detritus and on sensitivity and feeding plasticity of detritivore species.

Estrogenicity and intersex in juvenile rainbow trout (Oncorhynchus mykiss) exposed to Pine/Eucalyptus pulp and paper production effluent in Chile.

PubMed

Chiang, Gustavo; Barra, Ricardo; Díaz-Jaramillo, Mauricio; Rivas, Meyling; Bahamonde, Paulina; Munkittrick, Kelly R

2015-07-01

Pulp and paper mill effluents (PPMEs) have been shown to increase gonad size, cause early maturation, and disrupt hormone functions in native and non-native Chilean fish. In this study, we assessed reproductive (plasma vitellogenin; VTG, gonad development) and metabolic (ethoxyresorufin-O-deethylase activity; EROD) end points, relative liver size (LSI) and condition factor (K) of juvenile female and male rainbow trout exposed to effluents. Unlike previous studies, which have focus either on the specific effects of effluent on fish in laboratory exposures or biotic population statuses downstream of discharge sites, we simultaneously assessed the impacts of PPMES on trout using two approaches: (1) laboratory exposures of tertiary treated PPME produced from processing Eucalyptus globulus or Pinus radiata; and (2) in situ bioassay downstream of the combined discharge of the same pulp mill. Despite an increase in the average gonadosomatic index (GSI) in exposed fish, no statistical differences in gonad size between exposed and unexposed individuals was detected. However, both female and male fish exposed to effluents showed significantly higher concentrations of plasma VTG, so more in fish exposed to Eucalyptus-based effluent when compared to Pinus PPME. In addition, male fish showed intersex characteristics in all exposure assays (Eucaliptus and Pinus) and, despite the low concentration of effluent in the river (<1% [v/v]), similar responses were observed in the caged fish. Finally, EROD activity was induced in both in situ exposures and laboratory assays at the higher PPME concentration (60-85% PPME). This study confirms estrogenic effects in Chilean fish exposed to PPME and the necessity for biological effects monitoring in addition to the assessment of physical-chemical endpoints as required in current government regulations. Copyright © 2015 Elsevier B.V. All rights reserved.

Soil carbon storage in silvopasture and related land-use systems in the brazilian cerrado.

PubMed

Tonucci, Rafael G; Nair, P K Ramachandran; Nair, Vimala D; Garcia, Rasmo; Bernardino, Fernando S

2011-01-01

Silvopastoral management of fast-growing tree plantations is becoming popular in the Brazilian Cerrado (savanna). To understand the influence of such systems on soil carbon (C) storage, we studied C content in three aggregate size classes in six land-use systems (LUS) on Oxisols in Minas Gerais, Brazil. The systems were a native forest, a treeless pasture, 24- and 4-yr-old eucalyptus ( sp.) plantations, and 15- and 4-yr-old silvopastures of fodder grass plus animals under eucalyptus. From each system, replicated soil samples were collected from four depths (0-10, 10-20, 20-50, and 50-100 cm), fractionated into 2000- to 250-, 250- to 53-, and <53-μm size classes representing macroaggregates, microaggregates, and silt + clay, respectively, and their C contents determined. Macroaggregate was the predominant size fraction under all LUS, especially in the surface soil layers of tree-based systems. In general, C concentrations (g kg soil) in the different aggregate size fractions did not vary within the same depth. The soil organic carbon (SOC) stock (Mg C ha) to 1-m depth was highest under pasture compared with other LUS owing to its higher soil bulk density. The soils under all LUS had higher C stock compared with other reported values for managed tropical ecosystems: down to 1 m, total SOC stock values ranged from 461 Mg ha under pasture to 393 Mg ha under old eucalyptus. Considering the possibility for formation and retention of microaggregates within macroggregates in low management-intensive systems such as silvopasture, the macroaggregate dynamics in the soil seem to be a good indicator of its C storage potential. American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.

Exotic Eucalyptus leaves are preferred over tougher native species but affect the growth and survival of shredders in an Atlantic Forest stream (Brazil).

PubMed

Kiffer, Walace P; Mendes, Flavio; Casotti, Cinthia G; Costa, Larissa C; Moretti, Marcelo S

2018-01-01

We evaluated the effect of leaves of native and exotic tree species on the feeding activity and performance of the larvae of Triplectides gracilis, a typical caddisfly shredder in Atlantic Forest streams. Leaves of four native species that differ in chemistry and toughness (Hoffmannia dusenii, Miconia chartacea, Myrcia lineata and Styrax pohlii) and the exotic Eucalyptus globulus were used to determine food preferences and rates of consumption, production of fine particulate organic matter (FPOM), growth and survival of shredders. We hypothesized that the consumption rates of leaves of Eucalyptus and their effects on the growth and survival of shredders could be predicted by leaf chemistry and toughness. The larvae preferred to feed on soft leaves (H. dusenii and M. chartacea) independently of the content of nutrients (N and P) and secondary compounds (total phenolics). When such leaves were absent, they preferred E. globulus and did not consume the tough leaves (M. lineata and S. pohlii). In monodietary experiments, leaf consumption and FPOM production differed among the studied leaves, and the values observed for the E. globulus treatments were intermediate between the soft and tough leaves. The larvae that fed on H. dusenii and M. chartacea grew constantly over five weeks, while those that fed on E. globulus lost biomass. Larval survival was higher on leaves of H. dusenii, M. chartacea and S. pohlii than on E. globulus and M. lineata leaves. Although E. globulus was preferred over tougher leaves, long-term consumption of leaves of the exotic species may affect the abundance of T. gracilis in the studied stream. Additionally, our results suggest that leaf toughness can be a determining factor for the behavior of shredders where low-quality leaves are abundant, as in several tropical streams.