The effect of a beta-mannanase-based enzyme on growth performance and humoral immune response of broiler chickens fed diets containing graded levels of whole dates.

PubMed

Zangiabadi, Hamidreza; Torki, Mehran

2010-08-01

The use of low-quality whole dates in broilers' diets was evaluated. The apparent metabolizable energy (AMEn) of whole dates and date pits was determined with or without a beta-mannanase-based enzyme (Hemicell--a registered trademark of ChemGen Corp., Gaithersburg, MD. Dried Bacillus lentus fermentation solubles with 158 million units/kg minimum enzyme activity. Recommended usage rate is 0.4 g kg(-1) of feed. ChemGen Corp., Gaithersburg, MD, USA). A 3 x 2 factorial arrangement consisting of whole dates (0, 175, and 350 g kg(-1)) and enzyme (0 or 0.4 g kg(-1)) was performed to assess the effect of the enzyme on the nutritive value of whole dates (experiment 2) in broilers' diet. Enzyme improved AMEn contents of the whole dates and date pits. The diets containing 175 or 350 g kg(-1) whole dates produced body weight gain (BWG) similar to those obtained without dates. Enzyme improved BWG and feed conversion ratio throughout the experimental period. In conclusion, whole dates can be included in broiler diets at levels up to 350 g kg(-1). In addition, beta-mannanase supplementation would have beneficial effects on performance and immunity in birds fed on corn-soy or corn-soy-whole dates diets.

75 FR 19983 - National Heart, Lung, and Blood Institute; Notice of Closed Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-16

... Cardiovascular Conditions (GenTAC). Date: May 12, 2010. Time: 10 a.m. to 1 p.m. Agenda: To review and evaluate... Emphasis Panel, NHLBI Cardiovascular Outcomes Research Coordinating Unit. Date: May 25, 2010. Time: 8 a.m... Blood Institute Special Emphasis Panel, NHLBI Centers for Cardiovascular Outcomes Research. Date: May 25...

Environmental Impact Research Program. Proceedings of the US Army Corps of Engineers Riparian Zone Restoration and Management Workshop Held in San Antonio, Texas on 24-27 February 1986

DTIC Science & Technology

1988-02-01

Dwayne G. Lee, CE, was Commander and Director of WES. Dr. Robert W. Whalin was Technical Director. This report should be cited as follows: Martin, Chester...Environmental features for streambank protection projects. U.S. Army Eng. Waterways Exp. Sta., Tech. Rep. E-84-11. 150 pp. Johnson , R. R., and D. A. Jones...Serv. Gen. Tech. Rep. RM-43. 217 pp. 57 Johnson , R. R., and J. F. McCormick (tech. coords.). 1978. Strategies for protection and management of

Sex, genes, and heat: triggers of diversity.

PubMed

Western, P S; Sinclair, A H

2001-11-01

In vertebrates, sex is determined by a surprising variety of mechanisms. In many reptiles, the primary testis or ovary-determining trigger is regulated by egg incubation temperature. This temperature dependent sex determining (TSD) mechanism occurs in all crocodilians and marine turtles examined to date and is common in terrestrial turtles and viviparous lizards (Ewert et al. 1994. J Exp Zool 270:3-15; Lang and Andrews. 1994. J Exp Biol 270:28-44; Mrosovsky. 1994. J Exp Zool 270:16-27; Pieau. 1996. Bioessays 18:19-26; Viets et al. 1994. J Exp Zool 270:45-56; Wibbels et al. 1998. J Exp Zool 281:409-416). In contrast, sex in mammals and birds is determined chromosomally (CSD). Despite these differences, morphological development of the gonads in all these vertebrate groups appears to have been conserved through evolution. Therefore, the genetic mechanisms triggering sex determination appear not to have been conserved through evolution, although the basic genetic pathway controlling the morphological differentiation of the gonads appears to have been conserved. Copyright 2001 Wiley-Liss, Inc.

Genes affecting heading date in cocksfoot (Dactylis glomerata)

USDA-ARS?s Scientific Manuscript database

Several genes cause well known effects on heading date in cool-season forages: Vrn1, Constans, and FloweringTime. Vrn1 is a MADs box transcription factor that is induced upon vernalization and necessary for flowering. Constans genes are induced upon long days in cool-season grasses and induce exp...

Gen IV Materials Handbook Beta Release for Structural and Functional Evaluation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ren, Weiju; Luttrell, Claire

2006-09-12

Development of the Gen IV Materials Handbook is briefly summarized up to date. Current status of the Handbook website construction is described. The developed Handbook components and access control of the beta version are discussed for the present evaluation release. Detailed instructions and examples are given to provide guidance for evaluators to browse the constructed parts and use all the currently developed functionalities of the Handbook in evaluation.

78 FR 78347 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-26

..., 2013 Triennial Market Power Analysis of SGOC Southwest MBR Sellers for the Southwest Region. Filed Date... Numbers: ER14-630-000. Applicants: AlphaGen Power LLC. Description: Market-based rate application to be... Notice of succession-- Reactive Power Tariff to be effective 3/1/2014. Filed Date: 12/16/13. Accession...

The Three Wars of Lt. Gen. George E. Stratemeyer. His Korean War Diary

DTIC Science & Technology

1999-01-01

Powers (SCAP), he exercised command over all occupation forces and, in essence , ruled Japan; as Commander-in-Chief, Far East Command (CINCFE), he exercised... essence , he was attempting to make national policy himself and was giving the Chinese an ultimatum that the war would be extended to their mainland...LT. GEN. GEORGE E. STRATEMEYER : HIS KOREAN WAR DIARY 45. The Sykes report was, in essence , General Stratemeyer’s diary entries for those dates with

78 FR 26004 - Notice of Availability; Draft Environmental Impact Statement for the FutureGen 2.0 Project

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

... floodplain and wetland review requirements (10 CFR part 1022). DATES: DOE invites the public to comment on... commercial operations after this date. The oxy-combustion plant would be built on a 263-acre existing power... would be used for the injection facilities, associated infrastructure and buildings, and access roads...

77 FR 47617 - Change of Names Given for the Performance Review Board for the Department of the Air Force.

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-09

... for Acquisitions with Lt Gen Basla, Chief, Information Dominance and Chief Information Officer, Office of the Secretary of the Air Force. FOR FURTHER INFORMATION CONTACT: Please direct any written... the Air Force. DATES: Effective Date: November 6, 2012. SUPPLEMENTARY INFORMATION: Pursuant to 5 U.S.C...

Micromachined Thermoelectric Sensors and Arrays and Process for Producing

NASA Technical Reports Server (NTRS)

Foote, Marc C. (Inventor); Jones, Eric W. (Inventor); Caillat, Thierry (Inventor)

2000-01-01

Linear arrays with up to 63 micromachined thermopile infrared detectors on silicon substrates have been constructed and tested. Each detector consists of a suspended silicon nitride membrane with 11 thermocouples of sputtered Bi-Te and Bi-Sb-Te thermoelectric elements films. At room temperature and under vacuum these detectors exhibit response times of 99 ms, zero frequency D* values of 1.4 x 10(exp 9) cmHz(exp 1/2)/W and responsivity values of 1100 V/W when viewing a 1000 K blackbody source. The only measured source of noise above 20 mHz is Johnson noise from the detector resistance. These results represent the best performance reported to date for an array of thermopile detectors. The arrays are well suited for uncooled dispersive point spectrometers. In another embodiment, also with Bi-Te and Bi-Sb-Te thermoelectric materials on micromachined silicon nitride membranes, detector arrays have been produced with D* values as high as 2.2 x 10(exp 9) cm Hz(exp 1/2)/W for 83 ms response times.

Fast Risetime Reverse Bias Pulse Failures in SiC PN Junction Diodes

NASA Technical Reports Server (NTRS)

Neudeck, Philip G.; Fazi, Christian; Parsons, James D.

1996-01-01

SiC-based high temperature power devices are being developed for aerospace systems which will require high reliability. One behavior crucial to power device reliability. To date, it has necessarily been assumed to date is that the breakdown behavior of SiC pn junctions will be similar to highly reliable silicon-based pn junctions. Challenging this assumption, we report the observation of anomalous unreliable reverse breakdown behavior in moderately doped (2-3 x 10(exp 17) cm(exp -3)) small-area 4H- and 6H-SiC pn junction diodes at temperatures ranging from 298 K (25 C) to 873 K (600 C). We propose a mechanism in which carrier emission from un-ionized dopants and deep level defects leads to this unstable behavior. The fundamental instability mechanism is applicable to all wide bandgap semiconductors whose dopants are significantly un-ionized at typical device operating temperatures.

Lifetime Exposure to a Constant Environment Amplifies the Impact of a Fructose-Rich Diet on Glucose Homeostasis during Pregnancy.

PubMed

Song, Aleida; Astbury, Stuart; Hoedl, Abha; Nielsen, Brent; Symonds, Michael E; Bell, Rhonda C

2017-03-25

The need to refine rodent models of human-related disease is now being recognized, in particular the rearing environment that can profoundly modulate metabolic regulation. Most studies on pregnancy and fetal development purchase and transport young females into the research facility, which after a short period of acclimation are investigated (Gen0). We demonstrate that female offspring (Gen1) show an exaggerated hyperinsulinemic response to pregnancy when fed a standard diet and with high fructose intake, which continues throughout pregnancy. Markers of maternal hepatic metabolism were differentially influenced, as the gene expression of acetyl-CoA-carboxylase was raised in Gen1 given fructose and controls, whereas glucose transporter 5 and fatty acid synthase expression were only raised with fructose. Gen1 rats weighed more than Gen0 throughout the study, although fructose feeding raised the percent body fat but not body weight. We show that long-term habituation to the living environment has a profound impact on the animal's metabolic responses to nutritional intervention and pregnancy. This has important implications for interpreting many studies investigating the influence of maternal consumption of fructose on pregnancy outcomes and offspring to date.

Attracting and maintaining the Y Generation in nursing: a literature review.

PubMed

Hutchinson, Dianne; Brown, Janie; Longworth, Karen

2012-05-01

This paper explores the literature related to attracting the Y Generation (Y Gen: people born between 1980 and 2000) to the nursing profession and retaining them in our current workforce. A comprehensive review of the literature supported the need for further research. Three searches were conducted and all relevant literature was reviewed by each researcher. Literature included in the review was chosen based on specific search-term inclusion. Structured searches were conducted with no limitations on publication type, date or language. Search engines used included: Australian Family and Society, CINAHL, Expanded Academic, Google Scholar, Medline, ProQuest and PubMed. A critical review of the literature, particularly empirical work on the subject has informed decision making regarding the research questions that remain to be explored. The literature revealed that the Y Gen is currently contributing to the nursing workforce demographics. Much discussion exists surrounding the integration of the Y Gen into the workplace along with the other three generations of nurses. There is also an abundance of descriptions of the Y Gen characteristics and values. There is, however, limited reference relating to what attracted this generation to nursing or what might retain them in the nursing workforce. The Y Gen is the largest generation to enter our workforce since the Baby Boomers. Health services need to recognize the needs of the Y Gen nurses and develop strategies to move the profession forward by preparing the current workforce and environment for a generation that is already here. The focus should be on their strengths with development made to structure a workforce that will support the Y Gen in their professional nursing role. Understanding what attracts the Y Gen to nursing, what managers can do to retain the Y Gen in nursing and how the nursing profession can support the Y Gen to assume a role in nursing and nursing governance will ensure that the retiring generation has left the nursing profession in capable hands. © 2011 Blackwell Publishing Ltd.

Novae as a Class of Transient X-ray Sources

NASA Technical Reports Server (NTRS)

Mukai, K.; Orio, M.; Valle, M. Della

2007-01-01

Motivated by the recently discovered class of faint (10(exp 34)-10(exp 35) ergs/s) X-ray transients in the Galactic Center region, we investigate the 2-10 keV properties of classical and recurrent novae. Existing data are consistent with the idea that all classical novae are transient X-ray sources with durations of months to years and peak luminosities in the 10(exp 34)-10(exp 35)ergs/s range. This makes classical novae a viable candidate class for the faint Galactic Center transients. We estimate the rate of classical novae within a 15 arcmin radius region centered on the Galactic Center (roughly the field of view of XMM-Newton observations centered on Sgr A*) to be approx.0.1 per year. Therefore, it is plausible that some of the Galactic Center transients that have been announced to date are unrecognized classical novae. The continuing monitoring of the Galactic Center region carried out by Chandra and XMM-Newton may therefore provide a new method to detect classical novae in this crowded and obscured region, an

Search for Antihelium with the BESS-Polar Spectrometer

NASA Technical Reports Server (NTRS)

Sasaki, M.; Mitchell, J. W.; Hams, T.; Abe, K.; Fuke, H.; Haino, S.; Hasegawa, M.; Horikoshi, A.; Itazaki, A.; Kim, K. C.;

On the 2012 Record Low Arctic Sea Ice Cover: Combined Impact of Preconditioning and an August Storm

NASA Technical Reports Server (NTRS)

Parkinson, Claire L.; Comiso, Josefino C.

2013-01-01

A new record low Arctic sea ice extent for the satellite era, 3.4 x 10(exp 6) square kilometers, was reached on 13 September 2012; and a new record low sea ice area, 3.01 x 10(exp 6) square kilometers was reached on the same date. Preconditioning through decades of overall ice reductions made the ice pack more vulnerable to a strong storm that entered the central Arctic in early August 2012. The storm caused the separation of an expanse of 0.4 x 10(exp 6) square kilometers of ice that melted in total, while its removal left the main pack more exposed to wind and waves, facilitating the main pack's further decay. Future summer storms could lead to a further acceleration of the decline in the Arctic sea ice cover and should be carefully monitored.

78 FR 34362 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-07

.... Applicants: RockGen Energy, LLC. Description: Revised Reactive Rate Schedule to be effective 6/1/ 2013. Filed... Appendix X. Filed Date: 5/30/13. Accession Number: 20130530-5247. Comments Due: 5 p.m. ET 6/20/13. Docket...

Femme Fatale: An Examination of the Role of Women in Combat and the Policy Implications for Future American Military Operations (Drew Paper Number 5, August 2009)

DTIC Science & Technology

2009-08-01

subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. 1. REPORT DATE...NUMBER OF PAGES 137 19a. NAME OF RESPONSIBLE PERSON a . REPORT unclassified b. ABSTRACT unclassified c. THIS PAGE unclassified Standard Form...298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 Air University Allen G. Peck, Lt Gen, Commander Air Force Research Institute John A . Shaud, Gen, PhD

77 FR 58978 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-25

...;and investigations, committee meetings, agency decisions and rulings, #0;delegations of authority..., telephone, fax, email, customer code, agency code, purchase order number, credit card number/exp. date and... submitting it to APFO. Information collected is used to process fiscal obligations, communicate with the...

NextGen Weather Processor Architecture Study

DTIC Science & Technology

2010-03-09

achieved in a staged fashion , ideally with new components coming on-line in time to replace existing capabilities prior to their end-of-life dates... fashion , ideally with new components coming on-line in time to replace existing capabilities prior to their end-of-life dates. As part of NWP Segment...weather capabilities. These objectives are to be achieved in a staged fashion , ideally with new components coming on-line in time to replace existing

Directional Limits on Persistent Gravitational Waves Using LIGO S5 Science Data

NASA Technical Reports Server (NTRS)

Abadie, J.; Abbott, B. P.; Abbott, R.; Abernathy, M.; Accadia, T.; Acernese, F.; Adams, C.; Adhikari, R.; Ajith, P.; Allen, B.;

A first determination of the surface density of galaxy clusters at very low x-ray fluxes

NASA Technical Reports Server (NTRS)

Rosati, Piero; Della Ceca, Roberta; Burg, Richard; Norman, Colin; Giacconi, Riccardo

1995-01-01

We present the first results of a serendipitous search for clusters of galaxies in deep ROSAT position sensitive proportional counter (PSPC) pointed observations at high Galactic latitude. The survey is being carried out using a wavelet-based detection algorithm which is not biased against extended, low surface brightness sources. A new flux-diameter limited sample of 10 cluster candidates has been created from approximately 3 deg(exp 2) surveyed area. Preliminary CCD observations have revealed that a large fraction of these candidates correspond to a visible enhancement in the galaxy surface density, and several others have been identified from other surveys. We believe these sources to be either low- to moderate-redshift groups or intermediate- to high-redshift clusters. We show X-ray and optical images of some of the clusters identified to date. We present, for the first time, the derived number density of the galaxy clusters to a flux limit of 1 x 10(exp -14) ergs cm(exp -2) s(exp -1) (0.5-2.0 keV). This extends the log N-log S of previous cluster surveys by more than one decade in flux. Results are compared to theoretical predictions for cluster number counts.

Evidence for contemporary plant mitoviruses

USDA-ARS?s Scientific Manuscript database

Mitoviruses have small RNA(+) genomes, replicate in mitochondria, and have to date been directly shown to infect only fungi. For this report, sequences that appear to represent approximately complete mitovirus genomes were discovered in plant transcriptome data at GenBank. At least 17 of the refined...

Can we reliably estimate managed forest carbon dynamics using remotely sensed data?

NASA Astrophysics Data System (ADS)

Smallman, Thomas Luke; Exbrayat, Jean-Francois; Bloom, A. Anthony; Williams, Mathew

2015-04-01

Forests are an important part of the global carbon cycle, serving as both a large store of carbon and currently as a net sink of CO2. Forest biomass varies significantly in time and space, linked to climate, soils, natural disturbance and human impacts. This variation means that the global distribution of forest biomass and their dynamics are poorly quantified. Terrestrial ecosystem models (TEMs) are rarely evaluated for their predictions of forest carbon stocks and dynamics, due to a lack of knowledge on site specific factors such as disturbance dates and / or managed interventions. In this regard, managed forests present a valuable opportunity for model calibration and improvement. Spatially explicit datasets of planting dates, species and yield classification, in combination with remote sensing data and an appropriate data assimilation (DA) framework can reduce prediction uncertainty and error. We use a Baysian approach to calibrate the data assimilation linked ecosystem carbon (DALEC) model using a Metropolis Hastings-Markov Chain Monte Carlo (MH-MCMC) framework. Forest management information is incorporated into the data assimilation framework as part of ecological and dynamic constraints (EDCs). The key advantage here is that DALEC simulates a full carbon balance, not just the living biomass, and that both parameter and prediction uncertainties are estimated as part of the DA analysis. DALEC has been calibrated at two managed forests, in the USA (Pinus taeda; Duke Forest) and UK (Picea sitchensis; Griffin Forest). At each site DALEC is calibrated twice (exp1 & exp2). Both calibrations (exp1 & exp2) assimilated MODIS LAI and HWSD estimates of soil carbon stored in soil organic matter, in addition to common management information and prior knowledge included in parameter priors and the EDCs. Calibration exp1 also utilises multiple site level estimates of carbon storage in multiple pools. By comparing simulations we determine the impact of site-level observations on uncertainty and error on predictions, and which observations are key to constraining ecosystem processes. Preliminary simulations indicate that DALEC calibration exp1 accurately simulated the assimilated observations for forest and soil carbon stock estimates including, critically for forestry, standing wood stocks (R2 = 0.92, bias = -4.46 MgC ha-1, RMSE = 5.80 MgC ha-1). The results from exp1 indicate the model is able to find parameters that are both consistent with EDC and observations. In the absence of site-level stock observations (exp2) DALEC accurately estimates foliage and fine root pools, while the median estimate of above ground litter and wood stocks (R2 = 0.92, bias = -48.30 MgC ha-1, RMSE = 50.30 MgC ha-1) are over- and underestimated respectively, site-level observations are within model uncertainty. These results indicate that we can estimate managed forests dynamics using remotely sensed data, particularly as remotely sensed above ground biomass maps become available to provide constraint to correct biases in woody accumulation.

Generalized Read-Across (GenRA) prediction using chemical and biological information (BOSC)

EPA Science Inventory

Read-across is a popular data gap filling technique within category and analogue approaches for regulatory purposes. Acceptance of read-across remains a challenge with several efforts underway for identifying and addressing uncertainties. To date, these approaches have been quali...

The electrolyte challenge for a direct methanol-air polymer electrolyte fuel cell operating at temperatures up to 200 C

NASA Technical Reports Server (NTRS)

Savinell, Robert; Yeager, Ernest; Tryk, Donald; Landau, Uziel; Wainright, Jesse; Gervasio, Dominic; Cahan, Boris; Litt, Morton; Rogers, Charles; Scherson, Daniel

1993-01-01

Novel polymer electrolytes are being evaluated for use in a direct methanol-air fuel cell operating at temperatures in excess of 100 C. The evaluation includes tests of thermal stability, ionic conductivity, and vapor transport characteristics. The preliminary results obtained to date indicate that a high temperature polymer electrolyte fuel cell is feasible. For example, Nafion 117 when equilibrated with phosphoric acid has a conductivity of at least 0.4 Omega(exp -1)cm(exp -1) at temperatures up to 200 C in the presence of 400 torr of water vapor and methanol vapor cross over equivalent to 1 mA/cm(exp 2) under a one atmosphere methanol pressure differential at 135 C. Novel polymers are also showing similar encouraging results. The flexibility to modify and optimize the properties by custom synthesis of these novel polymers presents an exciting opportunity to develop an efficient and compact methanol fuel cell.

75 FR 6195 - Combined Notice of Filings # 1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-08

... Management Company, LLC. Description: Thornwood Management Co submits revisions to its market-based rate... Management Company, LLC. Description: Thornwood Management Co, LLC submits the Updated Market Power Analysis...: Algonquin Tinker Gen Co et al submits a Notice of Name Change and Succession. Filed Date: 01/27/2010...

75 FR 60856 - The Future of Aviation Advisory Committee (FAAC) Subcommittee on Labor and World-Class Workforce...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-01

... of a culture of dignity and respect in workplace; and (3) the effect of NextGen on various workforces... the subcommittee. DATES: The meeting will be held on October 1, 2010, from 1 p.m. to 4 p.m. Eastern...

Evaluation of Barrier Cable Impact Pad Materials

DTIC Science & Technology

1988-03-01

INFORMATION CENTER CAMERON STATION ALEXANDRIA, VIRGINIA 22314 Unclassified SECURITY CLASSIFICATION OF THIS PAGE Form Approved REPORT DOCUMENTATION PAGE OMB...No. 0704-0188 _____________________________________________Exp. Date: Jun 30, 1986 la. REPORT SECURITY CLASSIFICATION lb. RESTRICTIVE MARKINGS...Unclassified 2a. SECURITY CLASSIFICATION AUTHORITY 3. DISTRIBUTION/AVAILABILITY OF REPORT 2b. DECLASSIFICATION/DOWNGRADING SCHEDULE Approved for public

Exploring the temporal structure of heterochronous sequences using TempEst (formerly Path-O-Gen).

PubMed

Rambaut, Andrew; Lam, Tommy T; Max Carvalho, Luiz; Pybus, Oliver G

2016-01-01

Gene sequences sampled at different points in time can be used to infer molecular phylogenies on a natural timescale of months or years, provided that the sequences in question undergo measurable amounts of evolutionary change between sampling times. Data sets with this property are termed heterochronous and have become increasingly common in several fields of biology, most notably the molecular epidemiology of rapidly evolving viruses. Here we introduce the cross-platform software tool, TempEst (formerly known as Path-O-Gen), for the visualization and analysis of temporally sampled sequence data. Given a molecular phylogeny and the dates of sampling for each sequence, TempEst uses an interactive regression approach to explore the association between genetic divergence through time and sampling dates. TempEst can be used to (1) assess whether there is sufficient temporal signal in the data to proceed with phylogenetic molecular clock analysis, and (2) identify sequences whose genetic divergence and sampling date are incongruent. Examination of the latter can help identify data quality problems, including errors in data annotation, sample contamination, sequence recombination, or alignment error. We recommend that all users of the molecular clock models implemented in BEAST first check their data using TempEst prior to analysis.

Three-dimensional Numerical Simulation of Venus' Cloud-level Convection

NASA Astrophysics Data System (ADS)

Sugiyama, K. I.; Nakajima, K.; Odaka, M.; Imamura, T.; Hayashi, Y. Y.; Ishiwatari, M.; Kawabata, T.

2015-12-01

Although some observational evidences have suggested the occurrence of convection in the lower part of Venus' cloud layer, its structure remains to be clarified. To date, a few numerical studies have examined the structure of convective motion (Baker et al., 1998, 2000; Imamura et al., 2014), but the model they utilized is two-dimensional. Here we report on the results of our numerical calculations performed in order to investigate possible three-dimensional structure of the convection. We use a convection resolving model developed by Sugiyama et al. (2009), which is used in the simulations of the atmospheric convection of Jupiter (Sugiyama et al., 2011,2014) and Mars (Yamashita et al., submitted). We perform two experiments. The first one, which we call Ext.B, is based on Baker et al. (1998). A constant turbulent mixing coefficient is used in the whole domain, and a constant thermal flux is given at the upper and lower boundaries as a substitute for infrared heating. The second one, which we call Exp.I, is based on Imamura et al. (2014). The sub-grid turbulence process is implemented by Klemp and Wilhelmson (1989), and an infrared heating profile obtained in a radiative-convective equilibrium calculation (Ikeda, 2011) is used. In both experiments, the averaged solar heating profile is used. The spatial resolution is 200 m in the horizontal direction and 125 m in the vertical direction. The domain covers 128km x 128km horizontally and altitudes from 40 km to 60 km. Obtained structures of convection moderately differ in the two experiments. Although the depth of convection layer is almost the same, the horizontal cell size of Exp.B is larger than that of Exp.I; the cell sizes in Exp.B and Exp.I are about 40 km and 25 km, respectively. The vertical motion in Exp.B is asymmetric; updrafts are widespread and weak (~3m/s), whereas downdrafts are narrow and strong (~10m/s). On the other hand, the vertical motion in Exp.I is nearly symmetric and weaker (~2m/s) compared with those in Exp.B. The difference of convective structure results from the different vertical distributions of implemented infrared heating. Namely, the intense downdrafts in Exp.B are forced by the strong cooling concentrated near the top of convection layer. In Exp.I, the heating is distributed in a thick layer, so that relatively symmetric vertical motion occurs.

Targeted amplicon sequencing (TAS): a scalable next-gen approach to multilocus, multitaxa phylogenetics.

PubMed

Bybee, Seth M; Bracken-Grissom, Heather; Haynes, Benjamin D; Hermansen, Russell A; Byers, Robert L; Clement, Mark J; Udall, Joshua A; Wilcox, Edward R; Crandall, Keith A

2011-01-01

Next-gen sequencing technologies have revolutionized data collection in genetic studies and advanced genome biology to novel frontiers. However, to date, next-gen technologies have been used principally for whole genome sequencing and transcriptome sequencing. Yet many questions in population genetics and systematics rely on sequencing specific genes of known function or diversity levels. Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequence large numbers of targeted gene regions from a large number of samples. Our TAS approach is easily scalable, simple in execution, neither time-nor labor-intensive, relatively inexpensive, and can be applied to a broad diversity of organisms and/or genes. Our TAS approach includes a bioinformatic application, BarcodeCrucher, to take raw next-gen sequence reads and perform quality control checks and convert the data into FASTA format organized by gene and sample, ready for phylogenetic analyses. We demonstrate our approach by sequencing targeted genes of known phylogenetic utility to estimate a phylogeny for the Pancrustacea. We generated data from 44 taxa using 68 different 10-bp multiplexing identifiers. The overall quality of data produced was robust and was informative for phylogeny estimation. The potential for this method to produce copious amounts of data from a single 454 plate (e.g., 325 taxa for 24 loci) significantly reduces sequencing expenses incurred from traditional Sanger sequencing. We further discuss the advantages and disadvantages of this method, while offering suggestions to enhance the approach.

Targeted Amplicon Sequencing (TAS): A Scalable Next-Gen Approach to Multilocus, Multitaxa Phylogenetics

PubMed Central

Bybee, Seth M.; Bracken-Grissom, Heather; Haynes, Benjamin D.; Hermansen, Russell A.; Byers, Robert L.; Clement, Mark J.; Udall, Joshua A.; Wilcox, Edward R.; Crandall, Keith A.

2011-01-01

Next-gen sequencing technologies have revolutionized data collection in genetic studies and advanced genome biology to novel frontiers. However, to date, next-gen technologies have been used principally for whole genome sequencing and transcriptome sequencing. Yet many questions in population genetics and systematics rely on sequencing specific genes of known function or diversity levels. Here, we describe a targeted amplicon sequencing (TAS) approach capitalizing on next-gen capacity to sequence large numbers of targeted gene regions from a large number of samples. Our TAS approach is easily scalable, simple in execution, neither time-nor labor-intensive, relatively inexpensive, and can be applied to a broad diversity of organisms and/or genes. Our TAS approach includes a bioinformatic application, BarcodeCrucher, to take raw next-gen sequence reads and perform quality control checks and convert the data into FASTA format organized by gene and sample, ready for phylogenetic analyses. We demonstrate our approach by sequencing targeted genes of known phylogenetic utility to estimate a phylogeny for the Pancrustacea. We generated data from 44 taxa using 68 different 10-bp multiplexing identifiers. The overall quality of data produced was robust and was informative for phylogeny estimation. The potential for this method to produce copious amounts of data from a single 454 plate (e.g., 325 taxa for 24 loci) significantly reduces sequencing expenses incurred from traditional Sanger sequencing. We further discuss the advantages and disadvantages of this method, while offering suggestions to enhance the approach. PMID:22002916

The oldest fossil mushroom.

PubMed

Heads, Sam W; Miller, Andrew N; Crane, J Leland; Thomas, M Jared; Ruffatto, Danielle M; Methven, Andrew S; Raudabaugh, Daniel B; Wang, Yinan

2017-01-01

A new fossil mushroom is described and illustrated from the Lower Cretaceous Crato Formation of northeast Brazil. Gondwanagaricites magnificus gen. et sp. nov. is remarkable for its exceptional preservation as a mineralized replacement in laminated limestone, as all other fossil mushrooms are known from amber inclusions. Gondwanagaricites represents the oldest fossil mushroom to date and the first fossil mushroom from Gondwana.

Sagitta

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Arrow; abbrev. Sge, gen. Sagittae; area 80 sq. deg.) A northern constellation which lies between Vulpecula and Aquila, and culminates at midnight in mid-July. Its origin dates back at least to ancient Greece, where it was identified (by different authorities) with arrows belonging to Eros, Apollo and Hercules in Greek mythology. The brightest stars of Sagitta were cataloged by Ptolemy (c. AD...

The oldest fossil mushroom

PubMed Central

Miller, Andrew N.; Crane, J. Leland; Thomas, M. Jared; Ruffatto, Danielle M.; Methven, Andrew S.; Raudabaugh, Daniel B.; Wang, Yinan

2017-01-01

A new fossil mushroom is described and illustrated from the Lower Cretaceous Crato Formation of northeast Brazil. Gondwanagaricites magnificus gen. et sp. nov. is remarkable for its exceptional preservation as a mineralized replacement in laminated limestone, as all other fossil mushrooms are known from amber inclusions. Gondwanagaricites represents the oldest fossil mushroom to date and the first fossil mushroom from Gondwana. PMID:28591180

Scorpius

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Scorpion; abbrev. Sco., gen. Scorpii; area 497 sq. deg.) A southern zodiacal constellation which lies between Ophiuchus and Ara, and culminates at midnight in early June. Its origin dates back to Sumerian times, when it was called Girtab, `the stinger', but today it is associated with the scorpion that, in Greek mythology, killed Orion the hunter—and the two constellations lie on opposite sid...

Centaurus

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Centaur; abbrev. Cen, gen. Centauri; area 1060 sq. deg.) A southern constellation which lies between Vela and Lupus, and surrounds Crux on three sides. It culminates at midnight in early April. Its origin dates back at least to ancient Greece, where it was identified with Chiron in Greek mythology. The brightest stars of Centaurus were cataloged by Ptolemy (c. AD 100-175) in the Almagest....

Orion

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Hunter; abbrev. Ori, gen. Orionis; area 594 sq. deg.) An equatorial constellation which lies between Taurus and Monoceros, and culminates at midnight in mid-December. Its origin dates back to Sumerian times, when it was identified with the hero Gilgamesh and his fight against the Bull of Heaven (represented by Taurus), but today it is associated with the son of Poseidon, in Greek mythology, ...

Capricornus

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Sea Goat; abbrev. Cap, gen. Capricorni; area 414 sq. deg.) A southern zodiacal constellation which lies between Sagittarius and Aquarius, and culminates at midnight in early August. The origin of the unusual constellation figure, which comprises the head and forelimbs of a goat and tail of a fish, dates back to Babylonian times and has also been associated in Greek mythology with Pan, who ha...

Sunspots: Wilson Effect

NASA Astrophysics Data System (ADS)

Maltby, P.; Murdin, P.

2000-11-01

The Wilson effect refers to the depressed appearance of SUNSPOTS when positioned close to the solar limb. The impression is that sunspots are cavities in the SOLAR PHOTOSPHERE. The reason is that the radiation we observe is coming from deeper layers in the sunspot than in the surrounding photosphere. The detection of this depression by Alexander Wilson dates back to 1769. The phenomenon is exp...

Growth and characterization of high-performance photorefractive BaTiO3 crystals

NASA Technical Reports Server (NTRS)

Warde, C.; Garrett, M. H.; Chang, J. Y.; Jenssen, H. P.; Tuller, H. L.

1991-01-01

Barium titanate has been used for many nonlinear optical applications primarily because it has high grain and high self-pumped phase conjugate reflectivities. However, barium titanate has had a relatively slow response time, and thus low sensitivity. Therefore, it has not been suited to real-time operations. In this report we will describe the modifications in crystal growth, doping, reduction, and poling that have produced barium titanate crystals with the fastest photorefractive response time reported to date, approximately 21 microseconds with a beam-coupling gain coefficient of 38.7 cm(exp -1) and the highest sensitivity reported to date of 3.44 cm(exp 3)/kJ. The sensitivity of these barium titanate crystals is comparable to or greater than other photorefractive oxides. We will show, for the first time, beam-coupling in barium titanate at video frame rates. We infer from response time measurements that barium titanate has a phonon limited mobility. Also, photorefractive response time measurements as a function of the crystallographic orientation and grating wave vector for our cobalt-doped oxygen reduced crystals indicate that their faster response time arise because of an increase in the free carrier lifetime.

HST-COS Observations on Hydrogen, Helium, Carbon, and Nitrogen Emission from the SN 1987A Reverse Shock

NASA Technical Reports Server (NTRS)

France, Kevin; McCray, Richard; Penton, Steven V.; Kirshner, Robert P.; Challis, Peter; Laming, J. Martin; Bouchet, Patrice; Chevalier, Roger; Garnavich, Peter M.; Fransson, Claes;

Influence of nitrogen fertilization and defoliation frequency on nitrogen constituents and feeding value of annual ryegrass.

PubMed

Zhang, Y; Bunting, L D; Kappel, L C; Hafley, J L

1995-08-01

Two experiments were conducted to determine the seasonal effects of N fertilizer (NF) and defoliation interval (DI) on specific N fractions within annual ryegrass (AR) and overall usage of AR N by growing lambs. In Exp. 1, NF (0, 112, 224, and 336 kg of N/ha) and DI of 2, 4, or 6 wk were factorially applied to AR plots. Both decreasing DI and increasing NF increased N levels in AR in a manner that depended on harvest date (NF or DI x harvest date interaction, P < .05). The proportions of .2% NaOH soluble N or soluble protein N were not affected (P > .05) by NF or DI. Although NF had no effect (P > .05) on in situ disappearance of DM, decreasing the DI seemed to reduce the severity of the season-induced reduction in DM disappearance (DI x harvest date interaction, P = .04). In Exp. 2, NF (0 or 224 kg of N/ha) and a DI of 2 or 6 wk were factorially applied to AR plots. Fresh, frozen AR was fed in semipurified diets (48% of DM as AR) to four wether lambs (23 +/- 1.3 kg) using a 4 x 4 Latin square. Diets including AR had greater ruminal DM and plant N digestibilities when AR had a 2-wk vs a 6-wk DI with NF but had reduced digestibilities when AR had a 2-wk vs a 6-wk DI without NF.(ABSTRACT TRUNCATED AT 250 WORDS)

Back-Up/ Peak Shaving Fuel Cell System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Staudt, Rhonda L.

2008-05-28

This Final Report covers the work executed by Plug Power from 8/11/03 – 10/31/07 statement of work for Topic 2: advancing the state of the art of fuel cell technology with the development of a new generation of commercially viable, stationary, Back-up/Peak-Shaving fuel cell systems, the GenCore II. The Program cost was $7.2 M with the Department of Energy share being $3.6M and Plug Power’s share being $3.6 M. The Program started in August of 2003 and was scheduled to end in January of 2006. The actual program end date was October of 2007. A no cost extension was grated.more » The Department of Energy barriers addressed as part of this program are: Technical Barriers for Distributed Generation Systems: o Durability o Power Electronics o Start up time Technical Barriers for Fuel Cell Components: o Stack Material and Manufacturing Cost o Durability o Thermal and water management Background The next generation GenCore backup fuel cell system to be designed, developed and tested by Plug Power under the program is the first, mass-manufacturable design implementation of Plug Power’s GenCore architected platform targeted for battery and small generator replacement applications in the telecommunications, broadband and UPS markets. The next generation GenCore will be a standalone, H2 in-DC-out system. In designing the next generation GenCore specifically for the telecommunications market, Plug Power is teaming with BellSouth Telecommunications, Inc., a leading industry end user. The final next generation GenCore system is expected to represent a market-entry, mass-manufacturable and economically viable design. The technology will incorporate: • A cost-reduced, polymer electrolyte membrane (PEM) fuel cell stack tailored to hydrogen fuel use • An advanced electrical energy storage system • A modular, scalable power conditioning system tailored to market requirements • A scaled-down, cost-reduced balance of plant (BOP) • Network Equipment Building Standards (NEBS), UL and CE certifications.« less

Sagittarius

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Archer; abbrev. Sgr, gen. Sagittarii; area 867 sq. deg.) A southern zodiacal constellation which lies between Ophiuchus and Capricornus, and culminates at midnight in early July. Its origin dates back to Sumerian times, when it was identified with Nergal, a god of war, but today it is associated with Crotus, son of the Greek god Pan and the inventor of archery, and it is shown on early celes...

Taurus

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Bull; abbrev. Tau, gen. Tauri; area 797 sq. deg.) A northern zodiacal constellation which lies between Aries and Orion, and culminates at midnight in late November. It is one of the oldest constellations, dating back to when the Sun was in that part of the sky at the vernal (spring) equinox, between about 4000 and 1800 BC. Later, in Greek mythology, it was identified with the form assumed by...

Pisces

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Fishes; abbrev. Psc, gen. Piscium; area 889 sq. deg.) A northern zodiacal constellation which lies between Pegasus and Cetus, and culminates at midnight in late September. Its origin dates back to Babylonian times and it is said to represent Aphrodite, the goddess of love in Greek mythology, and her son Eros, who jumped into the Euphrates to escape from the multi-headed Typhon and were turned...

Aquila

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Eagle; abbrev. Aql, gen. Aquilae; area 652 sq. deg.) An equatorial constellation that lies between Sagitta and Sagittarius, and culminates at midnight in mid-July. Its origin dates back to Babylonian times and it is said to represent the eagle of Zeus in Greek mythology, which carried the thunderbolts that Zeus hurled at his enemies and which snatched up Ganymede to become cup-bearer to the g...

Aquarius

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Water-bearer; abbrev. Aqr, gen. Aquarii; area 980 sq. deg.) A southern zodiacal constellation that lies between Pegasus and Piscis Austrinus, and culminates at midnight in late August. Its origin dates back to Babylonian times and it is said to represent Ganymede in Greek mythology, who was snatched up by Aquila (the eagle) on Zeus's behest, to become cup-bearer to the gods. Its brightest st...

Hercules

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(abbrev. Her, gen. Herculis; area 1225 sq. deg.) A northern constellation which lies between Draco and Ophiuchus, and culminates at midnight in mid-June. The origin of the constellation figure is uncertain, though its name dates back to ancient Greece, where it was identified with the strong man and hero of Greek mythology. Its brightest stars were cataloged by Ptolemy (c. AD 100-175) in the Alma...

Single Particle Transport Through Carbon Nanotube Wires: Effect of Defects and Polyhedral Cap

NASA Technical Reports Server (NTRS)

Anantram, M. P.; Govidan, T. R.

1999-01-01

The ability to manipulate carbon nanotubes with increasing precision has enabled a large number of successful electron transport experiments. These studies have primarily focussed on characterizing transport through both metallic and semiconducting wires. Tans et al. demonstrated ballistic transport in single-wall nanotubes for the first time, although the experimental configuration incurred large contact resistance. Subsequently, methods of producing low contact resistances have been developed and two terminal conductances smaller than 50 k-ohms have been repeatably demonstrated in single-wall and multi-wall nanotubes. In multi-wall nanotubes, Frank et al. demonstrated a resistance of approximately h/2e(exp 2) in a configuration where the outermost layer made contact to a liquid metal. This was followed by the work of de Pablo et al. where a resistance of h(bar)/27e(exp 2) (approximately 478 ohms) was measured in a configuration where electrical contact was made to many layers of a multi-wall nanotube. Frank et al. and Pablo et al. note that each conducting layer contributes a conductance of only 2e(exp 2)/h, instead of the 4e(exp 2)/h that a single particle mode counting picture yields. These small resistances have been obtained in microns long nanotubes, making them the best conducting molecular wires to date. The large conductance of nanotube wires stems from the fact that the crossing bands of nanotubes are robust to defect scattering.

A neutral hydrogen survey of the Hydra 1 cluster

NASA Technical Reports Server (NTRS)

Mcmahon, Pauline; Vangorkom, Jacqueline; Richter, Otto; Ferguson, Henry

1993-01-01

We are undertaking a project to image the entire volume of the Hydra 1 cluster of galaxies in neutral hydrogen using the VLA. This involves making a series of pointings spaced 30 min. (the half power beam width) apart, each observed at three velocity settings in order to span the whole velocity range of the cluster. The purpose of this survey is to determine the true distribution, both in space and velocity, of gas-rich systems and hence, to deduce what effects a dense environment may have on the evolution of these systems. Most surveys of clusters to date have been performed on optically selected samples. However, optically selected samples may provide misleading views of the distribution of gas-rich systems, since many low surface brightness galaxies have an abundance of neutral gas (Bothun et al. 1987, Giovanelli & Haynes 1989). The Hydra project is providing the first unbiased view of the HI distribution in a cluster of galaxies. Our 5 sigma sensitivity is 4.1 x 10(exp 7) solar M/beam, (assuming H(sub 0) = 75 km s(exp -1) Mpc(exp -1)) and our velocity resolution is 42 km s(exp -1). We have a spatial resolution of 45 sec., which means that only the largest galaxies are spatially resolved enough to determine HI disk size. Our coverage is about 50 percent of the central region plus eight other fields centered on bright spirals within about 2 deg. of the center.

Rate Coefficient Measurements of the Reaction CH3+O2+CH3O+O

NASA Technical Reports Server (NTRS)

Hwang, S. M.; Ryu, Si-Ok; DeWitt, K. J.; Rabinowitz, M. J.

1999-01-01

Rate coefficients for the reaction CH3 + O2 = CH3O + O were measured behind reflected shock waves in a series of lean CH4-O2-Ar mixtures using hydroxyl and methyl radical diagnostics. The rate coefficients are well represented by an Arrhenius expression given as k = (1.60(sup +0.67, -0.47)) X 10(exp 13) exp(- 15813 +/- 587 K/T)cc/mol s. This expression, which is valid in the temperature range 1575-1822 K, supports the downward trend in the rate coefficients that has been reported in recent determinations. All measurements to date, including the present study, have been to some extent affected by secondary reactions. The complications due to secondary reactions, choice of thermochemical data, and shock-boundary layer interactions that affect the determination of the rate coefficients are examined.

Improving the Optical Quality Factor of the WGM Resonator

NASA Technical Reports Server (NTRS)

Savchenkov, Anatoliy; Matsko, Andrey; Iltchenko, Vladimir

2008-01-01

Resonators usually are characterized with two partially dependent values: finesse (F) and quality factor (Q). The finesse of an empty Fabry-Perot (FP) resonator is defined solely by the quality of its mirrors and is calculated as F=piR(exp 1/2)/(1-R). The maximum up-to-date value of reflectivity R approximately equal to 1 - 1.6 x 10(exp -6) is achieved with dielectric mirrors. An FP resonator made with the mirrors has finesse F=1.9 x 10(exp 6). Further practical increase of the finesse of FP resonators is problematic because of the absorption and the scattering of light in the mirror material through fundamental limit on the reflection losses given by the internal material losses and by thermodynamic density fluctuations on the order of parts in 109. The quality factor of a resonator depends on both its finesse and its geometrical size. A one-dimensional FP resonator has Q=2 F L/lambda, where L is the distance between the mirrors and lambda is the wavelength. It is easy to see that the quality factor of the resonator is unlimited because L is unlimited. F and Q are equally important. In some cases, finesse is technically more valuable than the quality factor. For instance, buildup of the optical power inside the resonator, as well as the Purcell factor, is proportional to finesse. Sometimes, however, the quality factor is more valuable. For example, inverse threshold power of intracavity hyperparametric oscillation is proportional to Q(exp 2) and efficiency of parametric frequency mixing is proportional to Q(exp 3). Therefore, it is important to know both the maximally achievable finesse and quality factor values of a resonator. Whispering gallery mode (WGM) resonators are capable of achieving larger finesse compared to FP resonators. For instance, fused silica resonators with finesse 2.3 x 10(exp 6) and 2.8 x 10(exp 6) have been demonstrated. Crystalline WGM resonators reveal even larger finesse values, F=6.3 x 10(exp 6), because of low attenuation of light in the transparent optical crystals. The larger values of F and Q result in the enhancement of various nonlinear processes. Low-threshold Raman lasing, optomechanical oscillations, frequency doubling, and hyperparametric oscillations based on these resonators have been recently demonstrated. Theory predicts a possibility of nearly 10(exp 14) room-temperature optical Q-factors of optical crystalline WGM resonators, which correspond to finesse levels higher than 10(exp 9). Experiments have shown numbers a thousand times lower than that. The difference occurs due to media imperfections. To substantially reduce the optical losses caused by the imperfections, a specific, multi-step, asymptotic processing of the resonator is implemented. The technique has been initially developed to reduce microwave absorption in dielectric resonators. One step of the process consists of mechanical polishing performed after high temperature annealing. Several steps repeat one after another to lead to significant reduction in optical attenuation and, as a result, to the increase of Q-factor as well as finesse of the resonator which demonstrates a CaF2 WGM resonator with F greater than 10(exp 7) and Q greater than 10(exp 11).

The QUIET Instrument

NASA Technical Reports Server (NTRS)

Gaier, T.; Kangaslahti, P.; Lawrence, C. R.; Leitch, E. M.; Wollack, E. J.

2012-01-01

The Q/U Imaging ExperimenT (QUIET) is designed to measure polarization in the Cosmic Microwave Background, targeting the imprint of inflationary gravitational waves at large angular scales ( approx 1 deg.) . Between 2008 October and 2010 December, two independent receiver arrays were deployed sequentially on a 1.4 m side-fed Dragonian telescope. The polarimeters which form the focal planes use a highly compact design based on High Electron Mobility Transistors (HEMTs) that provides simultaneous measurements of the Stokes parameters Q, U, and I in a single module. The 17-element Q-band polarimeter array, with a central frequency of 43.1 GHz, has the best sensitivity (69 micro Ks(exp 1/2)) and the lowest instrumental systematic errors ever achieved in this band, contributing to the tensor-to-scalar ratio at r < 0.1. The 84-element W-band polarimeter array has a sensitivity of 87 micro Ks(exp 1/2) at a central frequency of 94.5 GHz. It has the lowest systematic errors to date, contributing at r < 0.01 (QUIET Collaboration 2012) The two arrays together cover multipoles in the range l approximately equals 25-975 . These are the largest HEMT-ba.sed arrays deployed to date. This article describes the design, calibration, performance of, and sources of systematic error for the instrument,

A novel KRT5 mutation associated with generalized severe epidermolysis bullosa simplex in a 2-year-old Chinese boy

PubMed Central

Zhang, Jia; Yan, Ming; Liang, Jianying; Li, Ming; Yao, Zhirong

2016-01-01

Mutations in keratin 5 (KRT5) or KRT14 genes are responsible for the most severe form of epidermolysis bullosa simplex (EBS), which is EBS generalized severe (EBS-gen sev). To date, only four pathogenic mutations (p.Arg165Ser and p.Lys199Asn in KRT5; p.Arg125Cys and p.Arg125His in KRT14) have been reported to be responsible for EBS-gen sev in the Chinese population. In the present study, a 2-year-old Chinese boy was clinically suspected to suffer from EBS, and thus Sanger sequencing was performed in the extracted genomic DNA samples from the patient, his parents and 100 healthy controls. A novel de novo heterozygous missense mutation c.503A>G (p.Glu168Gly) located at the N-terminal end segment of the 1A domain in KRT5 was identified by molecular analysis. In silico analysis tools were used to predict the pathogenicity of the novel missense mutation. A diagnosis of EBS-gen sev was thus confirmed according to the clinical presentations and molecular results. PMID:27882080

The Cretaceous-Tertiary Impact Crater and the Cosmic Projectile that Produced it

NASA Technical Reports Server (NTRS)

Sharpton, Virgil L.; Marin, Luis E.

1997-01-01

Evidence gathered to date from topographic data, geophysical data, well logs, and drill-core samples indicates that the buried Chicxulub basin, the source crater for the approximately 65 Ma Cretaceous-Tertiary (K/T) boundary deposits, is approximately 300 km in diameter. A prominent topographic ridge and a ring of gravity anomalies mark the position of the basin rim at approximately 150 km from the center. Wells in this region recovered thick sequences of impact-generated breccias at 200-300 m below present sea level. Inside the rim, which has been severely modified by erosion following impact, the subsurface basin continues to deepen until near the center it is approximately 1 km deep. The best planetary analog for this crater appears to be the 270 km-diameter Mead basin on Venus. Seismic reflection data indicate that the central zone of downward displacement and excavation (the transient crater is approximately 130 km in diameter, consistent with previous studies of gravity anomaly data). Our analysis of projectile characteristics utilizes this information, coupled with conventional scaling relationships, and geochemical constraints on the mass of extraterrestrial material deposited within the K/T boundary layer. Results indicate that the Chicxulub crater would most likely be formed by a long-period comet composed primarily of nonsilicate materials (ice, hydrocarbons, etc.) and subordinate amounts (less than or equal to 50 percent) primitive chondritic material. This collision would have released the energy equivalent to between 4 x 10(exp 8) and 4 x 10(exp 9) megatons of TNT. Studies of terrestrial impact rates suggest that such an event would have a mean production rate of approximately 1.25 x 10(exp -9) y(exp -1). This rate is considerably lower than that of the major mass extinctions over the last 250 million years (approximately 5 x 10(exp -7) y(exp -1). Consequently, while there is substantial circumstantial evidence establishing the cause-effect link between the Chicxulub basin forming event and the K/T biological extinctions, the results of our analysis do not support models of impact as a common or singular causative agent of mass extinctions on Earth.

Fiscal Year 2011 Program Plan

DTIC Science & Technology

2011-03-15

IPSA ) Division will document the most likely scenario for NextGen 2025 given their analysis to-date. This will include OIs, risks, research, and...Office (CAO), and the Net-Centric Operations Division (NCOD) will review the assumptions and concur with this set as appropriate. IPSA will also...capabilities that can be achieved with additional emphasis on research, risk, and policy issues. SII, CAO, and NCOD will confer with IPSA on the

Constitutive modeling for isotropic materials (HOST)

NASA Technical Reports Server (NTRS)

Lindholm, Ulric S.; Chan, Kwai S.; Bodner, S. R.; Weber, R. M.; Walker, K. P.; Cassenti, B. N.

1984-01-01

The results of the first year of work on a program to validate unified constitutive models for isotropic materials utilized in high temperature regions of gas turbine engines and to demonstrate their usefulness in computing stress-strain-time-temperature histories in complex three-dimensional structural components. The unified theories combine all inelastic strain-rate components in a single term avoiding, for example, treating plasticity and creep as separate response phenomena. An extensive review of existing unified theories is given and numerical methods for integrating these stiff time-temperature-dependent constitutive equations are discussed. Two particular models, those developed by Bodner and Partom and by Walker, were selected for more detailed development and evaluation against experimental tensile, creep and cyclic strain tests on specimens of a cast nickel base alloy, B19000+Hf. Initial results comparing computed and test results for tensile and cyclic straining for temperature from ambient to 982 C and strain rates from 10(exp-7) 10(exp-3) s(exp-1) are given. Some preliminary date correlations are presented also for highly non-proportional biaxial loading which demonstrate an increase in biaxial cyclic hardening rate over uniaxial or proportional loading conditions. Initial work has begun on the implementation of both constitutive models in the MARC finite element computer code.

FINAL DESIGN REVIEW REPORT Subcritical Experiments Gen 2, 3-ft Confinement Vessel Weldment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Romero, Christopher

A Final Design Review (FDR) of the Subcritical Experiments (SCE) Gen 2, 3-ft. Confinement Vessel Weldment was held at Los Alamos National Laboratory (LANL) on September 14, 2017. The review was a focused review on changes only to the confinement vessel weldment (versus a system design review). The changes resulted from lessons-learned in fabricating and inspecting the current set of confinement vessels used for the SCE Program. The baseline 3-ft. confinement vessel weldment design has successfully been used (to date) for three (3) high explosive (HE) over-tests, two (2) fragment tests, and five (5) integral HE experiments. The design teammore » applied lessons learned from fabrication and inspection of these vessel weldments to enhance fit-up, weldability, inspection, and fitness for service evaluations. The review team consisted of five (5) independent subject matter experts with engineering design, analysis, testing, fabrication, and inspection experience. The« less

Commissioning and Performance Analysis of WhisperGen Stirling Engine

NASA Astrophysics Data System (ADS)

Pradip, Prashant Kaliram

Stirling engine based cogeneration systems have potential to reduce energy consumption and greenhouse gas emission, due to their high cogeneration efficiency and emission control due to steady external combustion. To date, most studies on this unit have focused on performance based on both experimentation and computer models, and lack experimental data for diversified operating ranges. This thesis starts with the commissioning of a WhisperGen Stirling engine with components and instrumentation to evaluate power and thermal performance of the system. Next, a parametric study on primary engine variables, including air, diesel, and coolant flowrate and temperature were carried out to further understand their effect on engine power and efficiency. Then, this trend was validated with the thermodynamic model developed for the energy analysis of a Stirling cycle. Finally, the energy balance of the Stirling engine was compared without and with heat recovery from the engine block and the combustion chamber exhaust.

Worldwide Report, Arms Control

DTIC Science & Technology

1986-02-13

Proposal (Various sources, various dates) 49 PRAVDA Rounds Up Reaction, by V. Gerasimov, et al . 49 PCF’s Marchais Praises Proposals !" ’ ; ’’ 52...affairs. The first world reaction to M.S. Gorbachev’s statement al ; jady attests to the enthusiasm which our new initiatives have gen- erated in... BORBA , organ of the Socialist Alliance of Working People of Yugoslavia, names as the first step in (lie Implementation of this program the USSK’s

Physiological time model for predicting adult emergence of western corn rootworm (Coleoptera: Chrysomelidae) in the Texas High Plains.

PubMed

Stevenson, Douglass E; Michels, Gerald J; Bible, John B; Jackman, John A; Harris, Marvin K

2008-10-01

Field observations at three locations in the Texas High Plains were used to develop and validate a degree-day phenology model to predict the onset and proportional emergence of adult Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) adults. Climatic data from the Texas High Plains Potential Evapotranspiration network were used with records of cumulative proportional adult emergence to determine the functional lower developmental temperature, optimum starting date, and the sum of degree-days for phenological events from onset to 99% adult emergence. The model base temperature, 10 degrees C (50 degrees F), corresponds closely to known physiological lower limits for development. The model uses a modified Gompertz equation, y = 96.5 x exp (-(exp(6.0 - 0.00404 x (x - 4.0), where x is cumulative heat (degree-days), to predict y, cumulative proportional emergence expressed as a percentage. The model starts degree-day accumulation on the date of corn, Zea mays L., emergence, and predictions correspond closely to corn phenological stages from tasseling to black layer development. Validation shows the model predicts cumulative proportional adult emergence within a satisfactory interval of 4.5 d. The model is flexible enough to accommodate early planting, late emergence, and the effects of drought and heat stress. The model provides corn producers ample lead time to anticipate and implement adult control practices.

Nemerteans of the Vema-TRANSIT expedition: First data on diversity with description of two new genera and species

NASA Astrophysics Data System (ADS)

Chernyshev, Alexei V.; Polyakova, Neonila E.

2018-02-01

Approximately 30 nemertean specimens were obtained from the samples collected with an epibenthic sledge during the Vema-TRANSIT expedition (2014-2015) to the Vema Fracture Zone. A preliminary molecular phylogenetic analysis of eight samples revealed four eumonostiliferan and three tubulanid species. Abyssonemertes kajiharai gen. et sp. nov. and Nemertovema hadalis gen. et sp. nov. are described based on morphological and DNA (16S, 18S, 28S, COI, and H3) data; the latter species is the deepest known nemertean to date. It was collected in the Puerto Rico Trench from a depth of 8336-8339 m. A preliminary molecular phylogenetic analysis based on the five genes indicates that three tubulanid species form a monophyletic group with the only previously known abyssal tubulanid (Tubulanidae gen. sp. IZ 45557) from the abyssal plain adjacent to the Kuril-Kamchatka Trench, but the four eumonostiliferan species belong to three different clades. Additionally, a roadmap on the most pressing questions and issues on abyssal and hadal nemerteans is provided. This is based on the processing of the nemertean material collected during the four deep-sea expeditions SoJaBio, KuramBio I, SokhoBio, and KuramBio II and shall provide a direction for future studies.

Voluntary Binge Consumption of Ethanol in a Sweetened, Chocolate-Flavored Solution by Male and Female Adolescent Sprague Dawley Rats.

PubMed

Hosová, Dominika; Spear, Linda Patia

2017-03-01

The still maturing adolescent brain may be particularly vulnerable to lasting consequences of ethanol (EtOH) exposure. Yet, human adolescents are the age group most likely to engage in binge drinking (a pattern of drinking leading to blood EtOH concentrations (BECs) of 80 mg/dl or greater). Most studies to date assessing the long-term effects of adolescent EtOH exposure in outbred rodent populations have either used experimenter-administered EtOH to produce BECs in the binge range or assessed voluntary intake of EtOH at well below binge levels. Beginning with a modified schedule-induced polydipsia (SIP) procedure, this study examined the suitability of several approaches to induce voluntary binge-like consumption during adolescence in an outbred rat strain. Adolescent male and female Sprague Dawley rats were food deprived to 85% projected free-feeding weights beginning on postnatal day (P) 24 and were given 30 minutes of access to 10% EtOH in chocolate Boost ® or Boost ® alone daily from P28 to P41 (followed later by their daily allocation of food). Animals were tested within operant chambers (Exp. 1a, 1b and Exp. 2) or home and novel cages (Exp. 3). Animals received either scheduled delivery of banana pellets to examine SIP (Exp. 1a,b) or massed pellet presentation (Exp. 2 and Exp. 3). Blood samples were collected via the lateral tail vein on P33 and P41. Intakes produced BECs frequently in the binge range (>80 mg/dl) and modeled binge-like consumption patterns, with high consumption days typically followed by 1 to 2 days of lower consumption; this variability was less evident with Boost ® alone. Consumption was not schedule induced and was generally high across all studies, although consumption in males appeared to be particularly pronounced when animals were tested in the presence of their cage mate. Binge-like patterns of EtOH consumption were produced using these procedures in adolescent Sprague Dawley rats of both sexes and may prove to be a useful model for work examining the short- and long-term consequences of high levels of voluntary EtOH intake in adolescence. Copyright © 2017 by the Research Society on Alcoholism.

Plasma Deposited SiO2 for Planar Self-Aligned Gate Metal-Insulator-Semiconductor Field Effect Transistors on Semi-Insulating InP

NASA Technical Reports Server (NTRS)

Tabory, Charles N.; Young, Paul G.; Smith, Edwyn D.; Alterovitz, Samuel A.

1994-01-01

Metal-insulator-semiconductor (MIS) field effect transistors were fabricated on InP substrates using a planar self-aligned gate process. A 700-1000 A gate insulator of Si02 doped with phosphorus was deposited by a direct plasma enhanced chemical vapor deposition at 400 mTorr, 275 C, 5 W, and power density of 8.5 MW/sq cm. High frequency capacitance-voltage measurements were taken on MIS capacitors which have been subjected to a 700 C anneal and an interface state density of lxl0(exp 11)/eV/cq cm was found. Current-voltage measurements of the capacitors show a breakdown voltage of 107 V/cm and a insulator resistivity of 10(exp 14) omega cm. Transistors were fabricated on semi-insulating InP using a standard planar self-aligned gate process in which the gate insulator was subjected to an ion implantation activation anneal of 700 C. MIS field effect transistors gave a maximum extrinsic transconductance of 23 mS/mm for a gate length of 3 microns. The drain current drift saturated at 87.5% of the initial current, while reaching to within 1% of the saturated value after only 1x10(exp 3). This is the first reported viable planar InP self-aligned gate transistor process reported to date.

A Catalog of Coronal "EIT Wave" Transients

NASA Technical Reports Server (NTRS)

Thompson, B. J.; Myers, D. C.

2009-01-01

Solar and Heliospheric Observatory (SOHO) Extreme ultraviolet Imaging Telescope (EIT) data have been visually searched for coronal "EIT wave" transients over the period beginning from 1997 March 24 and extending through 1998 June 24. The dates covered start at the beginning of regular high-cadence (more than one image every 20 minutes) observations, ending at the four-month interruption of SOHO observations in mid-1998. One hundred and seventy six events are included in this catalog. The observations range from "candidate" events, which were either weak or had insufficient data coverage, to events which were well defined and were clearly distinguishable in the data. Included in the catalog are times of the EIT images in which the events are observed, diagrams indicating the observed locations of the wave fronts and associated active regions, and the speeds of the wave fronts. The measured speeds of the wave fronts varied from less than 50 to over 700 km s(exp -1) with "typical" speeds of 200-400 km s(exp -1).

Plasma Wind Tunnel Testing of Electron Transpiration Cooling Concept

DTIC Science & Technology

2017-02-28

AFRL-AFOSR-UK-TR-2017-0012 Plasma Wind Tunnel Testing of Electron Transpiration Cooling Concept Olivier Chazot INSTITUT VON KARMAN DE DYNAMIQUE DES...28-02-2017 2. REPORT TYPE Final 3. DATES COVERED (From - To) 01 Dec 2015 to 30 Nov 2016 4. TITLE AND SUBTITLE Plasma Wind Tunnel Testing of Electron ...Aeronautics and Aerospace Department B-1640 Rhode Saint Genèse Belgium Internal Ref: ARR 1605 February 2017 Plasma Wind Tunnel Testing of Electron

The Role of recA Protein in the Multiplicity Reactivation Pathway of Phage T4.

DTIC Science & Technology

1983-01-01

shown below: HARRIS BERNSTEIN DATE Professor of Medical Molecular Microbiology i To Jane and Brian they make it all worthwhile Aeoession For NTiSi...Department of Molecular and Medical Microbiology , University of Arizona College of Medicine, Tucson, Arizona, 85724. Media The following growth media...M. and Prescott , C. (1983) Inducable expression of a gene specific to the recF pathway for recombination in Escherichia coli K12. Mol Gen Genet 190

Joint Force Quarterly. Issue 67, 4th Quarter, October 2012

DTIC Science & Technology

2012-10-01

a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. 1. REPORT DATE 2012 2...Defense Douglas N. Hime Naval War College Mark H. Jacobsen Marine Corps Command and Staff College Daniel T. Kuehl Information Resources Management...Norris Air Command and Staff College Col Mark Pizzo, USMC (Ret.) National War College James A . Schear Office of the Secretary of Defense LtGen Bernard

Project CHECO Southeast Asia Report. Ranch Hand: Herbicide Operations in SEA

DTIC Science & Technology

1971-07-13

Fortunately, the value of collecting and documenting our SEA expe-Uriences was recognized at an early date. In 1962 , Hq USAF directed CINCPACAF to...January 1962 to carry out the specific operations plan published the previous month, code named Sillm bI I "Ranch Hand." The first Ranch Hand aircraft...flew missions from 12 January 1962 I through 20 March 1962 . These missions were conducted along Route 15 north- 7/ west of Saigon and in the Ca Mau

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

PubMed Central

Muzzarelli, Riccardo A. A.; El Mehtedi, Mohamad; Bottegoni, Carlo; Aquili, Alberto; Gigante, Antonio

2015-01-01

The present review article intends to direct attention to the technological advances made since 2009 in the area of genipin-crosslinked chitosan (GEN-chitosan) hydrogels. After a concise introduction on the well recognized characteristics of medical grade chitosan and food grade genipin, the properties of GEN-chitosan obtained with a safe, spontaneous and irreversible chemical reaction, and the quality assessment of the gels are reviewed. The antibacterial activity of GEN-chitosan has been well assessed in the treatment of gastric infections supported by Helicobacter pylori. Therapies based on chitosan alginate crosslinked with genipin include stem cell transplantation, and development of contraction free biomaterials suitable for cartilage engineering. Collagen, gelatin and other proteins have been associated to said hydrogels in view of the regeneration of the cartilage. Viability and proliferation of fibroblasts were impressively enhanced upon addition of poly-l-lysine. The modulation of the osteocytes has been achieved in various ways by applying advanced technologies such as 3D-plotting and electrospinning of biomimetic scaffolds, with optional addition of nano hydroxyapatite to the formulations. A wealth of biotechnological advances and know-how has permitted reaching outstanding results in crucial areas such as cranio-facial surgery, orthopedics and dentistry. It is mandatory to use scaffolds fully characterized in terms of porosity, pore size, swelling, wettability, compressive strength, and degree of acetylation, if the osteogenic differentiation of human mesenchymal stem cells is sought: in fact, the novel characteristics imparted by GEN-chitosan must be simultaneously of physico-chemical and cytological nature. Owing to their high standard, the scientific publications dated 2010–2015 have met the expectations of an interdisciplinary audience. PMID:26690453

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone.

PubMed

Muzzarelli, Riccardo A A; El Mehtedi, Mohamad; Bottegoni, Carlo; Aquili, Alberto; Gigante, Antonio

2015-12-11

The present review article intends to direct attention to the technological advances made since 2009 in the area of genipin-crosslinked chitosan (GEN-chitosan) hydrogels. After a concise introduction on the well recognized characteristics of medical grade chitosan and food grade genipin, the properties of GEN-chitosan obtained with a safe, spontaneous and irreversible chemical reaction, and the quality assessment of the gels are reviewed. The antibacterial activity of GEN-chitosan has been well assessed in the treatment of gastric infections supported by Helicobacter pylori. Therapies based on chitosan alginate crosslinked with genipin include stem cell transplantation, and development of contraction free biomaterials suitable for cartilage engineering. Collagen, gelatin and other proteins have been associated to said hydrogels in view of the regeneration of the cartilage. Viability and proliferation of fibroblasts were impressively enhanced upon addition of poly-l-lysine. The modulation of the osteocytes has been achieved in various ways by applying advanced technologies such as 3D-plotting and electrospinning of biomimetic scaffolds, with optional addition of nano hydroxyapatite to the formulations. A wealth of biotechnological advances and know-how has permitted reaching outstanding results in crucial areas such as cranio-facial surgery, orthopedics and dentistry. It is mandatory to use scaffolds fully characterized in terms of porosity, pore size, swelling, wettability, compressive strength, and degree of acetylation, if the osteogenic differentiation of human mesenchymal stem cells is sought: in fact, the novel characteristics imparted by GEN-chitosan must be simultaneously of physico-chemical and cytological nature. Owing to their high standard, the scientific publications dated 2010-2015 have met the expectations of an interdisciplinary audience.

A new tardigrade, Mutaparadoxipus duodigifinis gen. nov., sp. nov. (Heterotardigrada: Arthrotardigrada), from the Southeastern United States.

PubMed

Gross, Vladimir; Miller, William R; Hochberg, Rick

2014-07-10

A new genus and species of Arthrotardigrada is described from Florida, USA based on its unique adhesive pad/claw combinations. Mutaparadoxipus duodigifinis gen. nov., sp. nov., is characterized by well-developed, ventral secondary clavae that are adjacent to the mouth, pointed lateral and caudal alae, seminal receptacles with coiled ducts opening lateral to the gonopore, and all legs with digits bearing proximal adhesive pads. Distal claws are present on digits I-III of legs I-III, but are missing from digit IV. On leg IV, distal claws are present only on digits II & III. A single accessory point is present on claws II & III only. This is the fourth species discovered to date with proximal adhesive pads, increasing support for a clade of adhesive-padded arthrotardigrades, and is likely the sister taxon of Paradoxipus orzeliscoides. The incomplete set of claws may represent an evolutionary step in a progressive loss of claws hypothesized to have occurred within the Halechiniscidae. The subfamily Orzeliscinae is amended as a result.

Free-Piston Shock Tunnel Test Technique Development: An AEDC/DLR Cooperative Program

DTIC Science & Technology

2003-02-01

Calibration Data." AIAA-95-6039, April 1995. 15. Molvik, G. A., and Merkle , C. L. "A Set of Strongly Coupled, Upwind Algorithms for Com- puting Flows in...April 1997. h. Date of Termination: 28 April 2002. i. All Signing Officials, Title/Offices Represented, and Countries: (1) Herr Rolf Schreiber, Chief...agreement by the RTP MOU Executive Agents. The US RTP/EA Signature Signature Clinton V. Hom, Maj Gen. USAF Rolf Schreiber Name Name Principal Assistant

Commercial Contracting of Army Lodging and Leisure Activities.

DTIC Science & Technology

1985-05-22

worldwide. The deal is the largest single franchise agreement in Burger King’s 30-year history, and could gen- erate about $400 million in revenue by 1980.13...The Burger King outlets will be operated by AAFES. AAFES will pay Burger King $15,000 for each franchise and six percent of the gross sales as...the family alone AAFES is making a significant contribution to soldier morale. While a form of contracting franchising as practiced by AAFES to date

[Efficient identification and analysis of low and medium frequency repeats]. Progress report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jurka, J.

1991-08-28

The effective starting date of this grant was May 15. In the first three months of this project we focused primarily on organizational and technical aspects of our research which included: organization of the database of repeats in primates; preparation of software for rapid and sensitive search of novel repetitive elements in GenBank; purchase and installation of the Sun workstation; and research on the mammal-specific MAR1 family of repetitive elements (to be communicated in October).

(Efficient identification and analysis of low and medium frequency repeats)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jurka, J.

1991-08-28

The effective starting date of this grant was May 15. In the first three months of this project we focused primarily on organizational and technical aspects of our research which included: organization of the database of repeats in primates; preparation of software for rapid and sensitive search of novel repetitive elements in GenBank; purchase and installation of the Sun workstation; and research on the mammal-specific MAR1 family of repetitive elements (to be communicated in October).

Decline of the 2-10 keV Emission from Eta Carinae

NASA Technical Reports Server (NTRS)

Liburd, Jamar; Corcoran, Michael F.; Hamaguchi, Kenji; Gull, Theodore R.; Madura, Thomas; Teodoro, Mairan; Moffat, Anthony; Richardson, Noel; Russell, Chris; Pollock, Andrew;

Two-Photon/Laser-Induced Fluorescence (TP/LIF) sensor

NASA Technical Reports Server (NTRS)

Bradshaw, John D.

1994-01-01

The Two-Photon/Laser-Induced Fluorescence (TP/LIF) technique is based on the stepwise excitation of the OH transitions, X(exp 2)II, v(exp '') = 0 yields X(exp 2)II, v(exp '') = 1 (lambda = 2.9 microns) and X(exp 2)II v(exp '') = 1 yields A(exp2)Sigma, v' = 0 (lambda = 345 nm) with background free fluorescence monitoring of the A(exp 2)Sigma, v' = 0 yields X(exp 2)II, v(exp '') = 0 transition near 309 nm. This technique has awaited the advent of a suitable mid-infrared (2.9 microns) laser source. Turnable mid-IR lasers now exist that are capable of meeting the specifications required of a high sensitivity TP/LIF OH sensor.

Classification of commutator algebras leading to the new type of closed Baker-Campbell-Hausdorff formulas

NASA Astrophysics Data System (ADS)

Matone, Marco

2015-11-01

We show that there are {\\it 13 types} of commutator algebras leading to the new closed forms of the Baker-Campbell-Hausdorff (BCH) formula $$\\exp(X)\\exp(Y)\\exp(Z)=\\exp({AX+BZ+CY+DI}) \\ , $$ derived in arXiv:1502.06589, JHEP {\\bf 1505} (2015) 113. This includes, as a particular case, $\\exp(X) \\exp(Z)$, with $[X,Z]$ containing other elements in addition to $X$ and $Z$. The algorithm exploits the associativity of the BCH formula and is based on the decomposition $\\exp(X)\\exp(Y)\\exp(Z)=\\exp(X)\\exp({\\alpha Y}) \\exp({(1-\\alpha) Y}) \\exp(Z)$, with $\\alpha$ fixed in such a way that it reduces to $\\exp({\\tilde X})\\exp({\\tilde Y})$, with $\\tilde X$ and $\\tilde Y$ satisfying the Van-Brunt and Visser condition $[\\tilde X,\\tilde Y]=\\tilde u\\tilde X+\\tilde v\\tilde Y+\\tilde cI$. It turns out that $e^\\alpha$ satisfies, in the generic case, an algebraic equation whose exponents depend on the parameters defining the commutator algebra. In nine {\\it types} of commutator algebras, such an equation leads to rational solutions for $\\alpha$. We find all the equations that characterize the solution of the above decomposition problem by combining it with the Jacobi identity.

Active Microparticle Media

DTIC Science & Technology

1992-09-01

if a(r,t) is any of these quantitites, then 7 a(r,t) = a0 + al(rwl) exp(- iwlt ) + al(r’w2 ) exp(-iw2 t) + 02 (r,w_) exp(-iwt) + a2 (r,w+) exp(-iw+t...02 (r,o) + a2 (r,2wI) exp(-2iwlt) + a2 (r,2w2 ) exp(-2iw2 t) + 0 3 (r,wl)exp(- iwlt ) + G3 (rgu 2 )exp(-1w2 t) + c.c. (6) where ao is the equilibrium

On the equilibrium state of a small system with random matrix coupling to its environment

NASA Astrophysics Data System (ADS)

Lebowitz, J. L.; Pastur, L.

2015-07-01

We consider a random matrix model of interaction between a small n-level system, S, and its environment, a N-level heat reservoir, R. The interaction between S and R is modeled by a tensor product of a fixed n× n matrix and a N× N Hermitian random matrix. We show that under certain ‘macroscopicity’ conditions on R, the reduced density matrix of the system {{ρ }S}=T{{r}R}ρ S\\cup R(eq), is given by ρ S(c)˜ exp \\{-β {{H}S}\\}, where HS is the Hamiltonian of the isolated system. This holds for all strengths of the interaction and thus gives some justification for using ρ S(c) to describe some nano-systems, like biopolymers, in equilibrium with their environment (Seifert 2012 Rep. Prog. Phys. 75 126001). Our results extend those obtained previously in (Lebowitz and Pastur 2004 J. Phys. A: Math. Gen. 37 1517-34) (Lebowitz et al 2007 Contemporary Mathematics (Providence RI: American Mathematical Society) pp 199-218) for a special two-level system.

Detection of New Dissociative Electron Attachment Channels in NO

NASA Technical Reports Server (NTRS)

Orient, O. J.; Chutjian, A.

1995-01-01

Three dissociative electron attachment channels have been detected and identified in NO via measurement of the O minus (exp 2)P fragment energy. In addition to the known N((exp 2 D(exp 0)) + O minus (exp 2)P channel, two new channels N((exp 1 S(exp 0)) + 0 (2 P) and N(exp 2)P(exp 0) + O(exp 2)P were detected. Cross sections for each of the channels are reported by normalizing the scattering intensities to previously measured total cross sections. The experimental approach uses solenoidal magnetic confinement of the electrons and ions, and trochoidal energy analysis of the low-energy ions.

Analysis of Expressed Sequence Tags (EST) in Date Palm.

PubMed

Al-Faifi, Sulieman A; Migdadi, Hussein M; Algamdi, Salem S; Khan, Mohammad Altaf; Al-Obeed, Rashid S; Ammar, Megahed H; Jakse, Jerenj

2017-01-01

Expressed sequence tags (EST) were generated from a normalized cDNA library of the date palm Sukkari cv. to understand the high-quality and better field performance of this well-known commercial cultivar. A total of 6943 high-quality ESTs were generated, out of them 6671 are submitted to the GenBank dbEST (LIBEST_028537). The generated ESTs were assembled into 6362 unigenes, consisting of 494 (14.4%) contigs and 5868 (84.53%) singletons. The functional annotation shows that the majority of the ESTs are associated with binding (44%), catalytic (40%), transporter (5%), and structural molecular (5%) activities. The blastx results show that 73% of unigenes are significantly similar to known plant genes and 27% are novel. The latter could be of particular interest in date palm genetic studies. Further analysis shows that some ESTs are categorized as stress/defense- and fruit development-related genes. These newly generated ESTs could significantly enhance date palm EST databases in the public domain and are available to scientists and researchers across the globe. This knowledge will facilitate the discovery of candidate genes that govern important developmental and agronomical traits in date palm. It will provide important resources for developing genetic tools, comparative genomics, and genome evolution among date palm cultivars.

All-Sky Search for Periodic Gravitational Waves in the Full S5 LIGO Data

NASA Technical Reports Server (NTRS)

Abadie, J.; Abbott, B. P.; Abbott, R.; Abbott, T. D.; Abernathy, M.; Accadia, T.; Acernese, F.; Adams, C.; Adhikari, R.; Affeldt, C.;

Photoionization Modeling of Oxygen K Absorption in the Interstellar Medium: The Chandra Grating Spectra of XTE J1817-330

NASA Technical Reports Server (NTRS)

Gatuzz, E.; Garcia, J.; Menodza, C.; Kallman, T. R.; Witthoeft, M.; Lohfink, A.; Bautista, M. A.; Palmeri, P.; Quinet, P.

2013-01-01

We present detailed analyses of oxygen K absorption in the interstellar medium (ISM) using four high-resolution Chandra spectra towards the X-ray low-mass binary XTE J1817-330. The 11-25 A broadband is described with a simple absorption model that takes into account the pileup effect and results in an estimate of the hydrogen column density. The oxygen K-edge region (21-25 A) is fitted with the physical warmabs model, which is based on a photoionization model grid generated with the XSTAR code with the most up-to-date atomic database. This approach allows a benchmark of the atomic data which involves wavelength shifts of both the K lines and photoionization cross sections in order to fit the observed spectra accurately. As a result we obtain: a column density of N(sub H) = 1.38 +/- 0.01 x 10(exp 21) cm(exp -2); ionization parameter of log xi = .2.70 +/- 0.023; oxygen abundance of A(sub O) = 0.689(exp +0.015./-0.010); and ionization fractions of O I/O = 0.911, O II/O = 0.077, and O III/O = 0.012 that are in good agreement with previous studies. Since the oxygen abundance in warmabs is given relative to the solar standard of Grevesse and Sauval (1998), a rescaling with the revision by Asplund et al. (2009) yields A(sub O) = 0.952(exp +0.020/-0.013, a value close to solar that reinforces the new standard. We identify several atomic absorption lines.K-alpha , K-beta, and K-gamma in O I and O II; and K-alpha in O III, O VI, and O VII--last two probably residing in the neighborhood of the source rather than in the ISM. This is the first firm detection of oxygen K resonances with principal quantum numbers n greater than 2 associated to ISM cold absorption.

The Sunyaev-Zel'dovich Effect Spectrum of Abell 2163

NASA Technical Reports Server (NTRS)

LaRoque, S. J.; Carlstrom, J. E.; Reese, E. D.; Holder, G. P.; Holzapfel, W. L.; Joy, M.; Grego, L.; Six, N. Frank (Technical Monitor)

2002-01-01

We present an interferometric measurement of the Sunyaev-Zel'dovich effect (SZE) at 1 cm for the galaxy cluster Abell 2163. We combine this data point with previous measurements at 1.1, 1.4, and 2.1 mm from the SuZIE experiment to construct the most complete SZE spectrum to date. The intensity in four wavelength bands is fit to determine the Compton y-parameter (y(sub 0)) and the peculiar velocity (v(sub p)) for this cluster. Our results are y(sub 0) = 3.56((sup +0.41+0.27)(sub -0.41-0.19)) X 10(exp -4) and v(sub p) = 410((sup +1030+460) (sub -850-440)) km s(exp -1) where we list statistical and systematic uncertainties, respectively, at 68% confidence. These results include corrections for contamination by Galactic dust emission. We find less contamination by dust emission than previously reported. The dust emission is distributed over much larger angular scales than the cluster signal and contributes little to the measured signal when the details of the SZE observing strategy are taken into account.

Early results from the MIT millimeter and sub-millimeter balloon-borne anisotropy measurement. [of cosmic microwave background radiation

NASA Technical Reports Server (NTRS)

Meyer, Stephan S.; Cheng, Edward S.; Page, Lyman A.

1991-01-01

The MIT balloon-borne bolometric search for Cosmic Microwave Background Radiation (CMBR) anisotropies places the most stringent constraints to date on fluctuations in the CMBR. Four maps of half of the Northern Hemisphere at 1.8, 1.1, 0.63 and 0.44 mm wavelength, have a beam size of 3.8 deg with a 1 sigma sensitivity of less than 0.1 mK (thermodynamic) per FOV in each of the first two channels. Analysis of the sky map at 1.8 mm wavelength using a likelihood ratio test for galactic latitudes of 15 deg and greater yields a 95 percent confidence level (CL) upper limit on fluctuations of the CMBR at DeltaT/T less than or equal to 1.6 x 10 exp -5 with a statistical power of 92 percent for Gaussian fluctuations at a correlation angle of 13 deg. Between 3 deg and 22 deg, the upper limit for fluctuations is DeltaT/T less than or equal to 4.0 x 10 exp -5 (95 percent CL).

Space-Borne Infrared Astronomy

NASA Technical Reports Server (NTRS)

Lange, Andrew E.

1997-01-01

The objective of this grant is to develop the Far IR Photometer (FIRP), one of four focal plane instruments on the IR Telescope in Space (IRTS). The IRTS was successfully launched in March 18, 1995 aboard the Japanese SFU platform. It surveyed the IR sky for approximately 40 days, and was eventually retrieved by NASA's STS. The FIRP succeeded in surveying approximately 5% of the sky in four bands centered at 150, 250, 400 and 700 microns. Several new technologies were developed using the funds from this grant, including: (1) a high performance gas-gap heat-switch, (2) a He-3 sorption refrigerator that is, to date, the only refrigerator to achieve sub-Kelvin temperatures in orbit, (3) high-sensitivity bolometric detectors with NEP less than 10-16 W(Hz(exp l/2)exp 1/2) when operated from a 300 mK heat sink, (4) readout electronics capable of providing DC stability for the bolometric detectors. Excess noise of unknown origin significantly reduced the sensitivity of the FIRP on orbit. Nevertheless, scientifically significant observations of the spectrum and temperature of the interstellar dust were made, and have been reported.

First measurement of helium on Mars: Implications for the problem of radiogenic gases on the terrestrial planets

NASA Technical Reports Server (NTRS)

Bowyer, S.; Chakrabarti, S.; Gladstone, G. R.; Mcdonald, J. S.

1994-01-01

The 108 photons of the Martian He 584 A airglow detected by the Extreme Ultraviolet Explorer satellite during a two-day exposure (22-23 Jan. 1993) correspond to the effective disk average intensity of 43 (+/-) 10 Rayleigh. Radiative transfer calculations, using a model atmosphere appropriate to the conditions of the observation and having an exospheric temperature of 210 (+/-) 20 K, result in an He mixing ratio of 1.1 (+/-) 0.4 ppm in the lower atmosphere. Nonthermal escape of helium is due to the following: electron impact ionization and pickup of He(+) by the solar wind; collisions with hot oxygen atoms; and charge exchange with molecular species with corresponding column loss rates of 1.4 x 10(exp 5), 3 x 10(exp 4), and 7 x 10(exp 3) cm(exp -2)s(exp -1), respectively. The lifetime of helium on Mars is 5 x 10(exp 4) yr. The He outgassing rate, coupled with the Ar-40 atmospheric abundance and with the K:U:Th ratio measured in the surface rocks, is used as input to a simple two-reservoir degassing model which presumes the loss of all argon accumulated in the atmosphere during the first Byr by large-scale impacts. The model results in total planet mass ratios of 10(exp -5) g/g for K, 2.3 x 10(exp -9) g/g for U, 8.5 x 10(exp -9) g/g for Th, 4 x 10(exp -10) g/g for He, and 1.5 x 10(exp -9) g/g for Ar-40. The predicted radiogenic heat flux is 2 erg cm(exp -2)s(exp -1). Similar modeling for Venus results in total plant mass ratios of 4.7 x 10(exp -5) g/g for K, 6.7 x 10(exp -9) g/g for U, 2.2 x 10(exp -8) g/g for Th, 1.3 x 10(exp -9) for He, 6.7 x 10(exp -9) g/g for Ar-40, and a radiogenic heat flux of 15 erg cm(exp -2)s(exp -1). The implications of these results are discussed. The modeling shows that the radioactive elements were not distributed uniformly in the protoplanetary nebula, and their relative abundances differ very much in the terrestrial planets.

Friction and Wear Properties of As-Deposited and Carbon Ion-Implanted Diamond Films

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa

1996-01-01

Recent work on the friction and wear properties of as-deposited and carbon ion-implanted diamond films was reviewed. Diamond films were produced by the microwave plasma chemical vapor deposition (CVD) technique. Diamond films with various grain sizes and surface roughnesses were implanted with carbon ions at 60 keV ion energy, resulting in a dose of 1.2 x 10(exp 17) carbon ions per cm(exp 2). Various analytical techniques, including Raman spectroscopy, proton recoil analysis, Rutherford backscattering, transmission and scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction, were utilized to characterize the diamond films. Sliding friction experiments were conducted with a polished natural diamond pin in contact with diamond films in the three environments: humid air (40% relative humidity), dry nitrogen (less than 1 percent relative humidity), and ultrahigh vacuum (10(exp -7) Pa). The CVD diamond films indeed have friction and wear properties similar to those of natural diamond in the three environments. The as-deposited, fine-grain diamond films can be effectively used as self-lubricating, wear-resistant coatings that have low coefficients of friction (0.02 to 0.04) and low wear rates (10(exp -7) to lO(exp -8) mm(exp 3) N(exp -1) m(exp -1)) in both humid air and dry nitrogen. However, they have high coefficients of friction (1.5 to 1.7) and a high wear rate (10(exp -4) mm(exp 7) N(exp -1) m(exp -1)) in ultrahigh vacuum. The carbon ion implantation produced a thin surficial layer (less than 0.1 micron thick) of amorphous, non-diamond carbon on the diamond films. In humid air and dry nitrogen, the ion-implanted, fine and coarse-grain diamond films have a low coefficient of friction (around 0.1) and a low wear rate (10(exp -7) mm(exp 3) N(exp -1) m(exp-1)). Even in ultrahigh vacuum, the presence of the non-diamond carbon layer reduced the coefficient of friction of fine-grain diamond films to 0.1 or lower and the wear rate to 10(exp -6) mm(exp 3) N(exp -1) m(exp -1). Thus, the carbon ion-implanted, fine-grain diamond films can be effectively used as wear-resistant, self-lubricating coatings not only in air and dry nitrogen, but also in ultrahigh vacuum.

Electrical characterization of 6H crystalline silicon carbide. M.S. Thesis Final Report

NASA Technical Reports Server (NTRS)

Lempner, Stephen E.

1994-01-01

Crystalline silicon carbide (SiC) substrates and epilayers, undoped as well as n- and p-doped, have been electrically characterized by performing Hall effect and resistivity measurements (van der Pauw) over the temperature range of approximately 85 K to 650 K (200 K to 500 K for p-type sample). By fitting the measured temperature dependent carrier concentration data to the single activation energy theoretical model: (1) the activation energy for the nitrogen donor ranged from 0.078 eV to 0.101 eV for a doping concentration range of 10(exp 17) cm(exp -3) to 10(exp 18) cm(exp -3) and (2) the activation energy for the aluminum acceptor was 0.252 eV for a doping concentration of 4.6 x 10(exp 18) cm(exp -3). By fitting the measured temperature dependent carrier concentration data to the double activation energy level theoretical model for the nitrogen donor: (1) the activation energy for the hexagonal site was 0.056 eV and 0.093 eV corresponding to doping concentrations of 3.33 x 10 (exp 17) cm(exp -3) and 1.6 x 10(exp 18) cm(exp -3) and (2) the activation energy for the cubic site was 0.113 and 0.126 eV corresponding to doping concentrations of 4.2 x 10(exp 17) cm(exp -3) and 5.4 x 10(exp 18) cm(exp -3).

Molecular systematics of Serrasalmidae: Deciphering the identities of piranha species and unraveling their evolutionary histories

USGS Publications Warehouse

Freeman, B.; Nico, L.G.; Osentoski, M.; Jelks, H.L.; Collins, T.M.

2007-01-01

Piranhas and their relatives have proven to be a challenging group from a systematic perspective, with difficulties in identification of species, linking of juveniles to adults, diagnosis of genera, and recognition of higher-level clades. In this study we add new molecular data consisting of three mitochondrial regions for museum vouchered and photo-documented representatives of the Serrasalmidae. These are combined with existing serrasalmid sequences in GenBank to address species and higher-level questions within the piranhas using parsimony and Bayesian methods. We found robust support for the monophyly of Serrasalmus manueli, but not for Serrasalmus gouldingi when GenBank specimens identified as S. gouldingi were included in the analysis. "Serrasalmus gouldingi" sequences in GenBank may, however, be misidentified. Linking of juveniles to adults of the same species was greatly facilitated by the addition of sequence data. Based on our sampling and identifications, our data robustly reject the monophyly of the genera Serrasalmus and Pristobrycon. We found evidence for a well-supported clade comprised of Serrasalmus, Pygocentrus, and Pristobrycon (in part). This clade was robustly supported in separate and combined analyses of gene regions, and was also supported by a unique molecular character, the loss of a tandem repeat in the control region. Analysis of specimens and a literature review suggest this clade is also characterized by the presence of a pre-anal spine and ectopterygoid teeth. A persistent polytomy at the base of this clade was dated using an independent calibration as 1.8 million years old, corresponding to the beginning of the Pleistocene Epoch, and suggesting an origin for this clade more recent than dates cited in the recent literature. The sister group to this clade is also robustly supported, and consists of Catoprion, Pygopristis, and Pristobrycon striolatus. If the term piranha is to refer to a monophyletic clade, it should be restricted to Serrasalmus, Pygocentrus, and Pristobrycon (in part), or expanded to include these taxa plus Pygopristis, Catoprion, and Pristobrycon striolatus. Copyright ?? 2007 Magnolia Press.

Modelling Temporal Variability in the Carbon Balance of a Spruce/Moss Boreal Forest

NASA Technical Reports Server (NTRS)

Frolking, S.; Goulden, M. L.; Wofsy, S. C.; Fan, S.-M.; Sutton, D. J.; Munger, J. W.; Bazzaz, A. M.; Daube, B. C.; Crill, P. M.; Aber, J. D.;

A method of solving simple harmonic oscillator Schroedinger equation

NASA Technical Reports Server (NTRS)

Maury, Juan Carlos F.

1995-01-01

A usual step in solving totally Schrodinger equation is to try first the case when dimensionless position independent variable w is large. In this case the Harmonic Oscillator equation takes the form (d(exp 2)/dw(exp 2) - w(exp 2))F = 0, and following W.K.B. method, it gives the intermediate corresponding solution F = exp(-w(exp 2)/2), which actually satisfies exactly another equation, (d(exp 2)/dw(exp 2) + 1 - w(exp 2))F = 0. We apply a different method, useful in anharmonic oscillator equations, similar to that of Rampal and Datta, and although it is slightly more complicated however it is also more general and systematic.

Common System and Software Testing Pitfalls

DTIC Science & Technology

2014-11-03

GEN- TTE -1) Target Platform Difficult to Access (GEN- TTE -2) → Inadequate Test Environments (GEN- TTE -3) Poor Fidelity of Test Environments (GEN- TTE -4...Inadequate Test Environment Quality (GEN- TTE -5) Test Environments Inadequately Tested (GEN- TTE -6) [new pitfall] Inadequate Test Configuration...Management (GEN- TTE -7) 29Common System/SW Testing PitfallsDonald G. Firesmith, 3 November 2014 General Pitfalls – Automated Testing [new pitfall category

United States Forces Korea > Home

Science.gov Websites

commander; Gen. Leem Ho Young, Combined Forces Command deputy commander; and Gen. Lee Sun-jin, Gen. Lee, Sun Forces Command deputy commander; and Gen. Lee Sun-jin, Gen. Lee, Sun-Jin, Chairman of the Republic of deputy commander; and Gen. Lee Sun-jin, Gen. Lee, Sun-Jin, Chairman of the Republic of Korea Joint Chiefs

Low-Temperature Rate Coefficients of C2H with CH4 and CD4 from 154 to 359 K

NASA Technical Reports Server (NTRS)

Opansky, Brian J.; Leone, Stephen R.

1996-01-01

Rate coefficients for the reaction C2H + CH4 yields C2H2 + CH3 and C2H + CD4 yields C2HD + CD3 are measured over the temperature range 154-359 K using transient infrared laser absorption spectroscopy. Ethynyl radicals are produced by pulsed laser photolysis of C2H2 in a variable temperature flow cell, and a tunable color center laser probes the transient removal of C2H (Chi(exp 2) Sigma(+) (0,0,0)) in absorption. The rate coefficients for the reactions of C2H with CH4 and CD4 both show a positive temperature dependence over the range 154-359 K, which can be expressed as k(sub CH4) = (1.2 +/- 0.1) x 10(exp -11) exp((-491 +/- 12)/T) and k(sub CD4) = (8.7 +/- 1.8) x 10(exp -12) exp((-650 +/- 61)/T) cm(exp 3) molecule(exp -1) s(exp -1), respectively. The reaction of C2H + CH4 exhibits a significant kinetic isotope effect at 300 K of k(sub CH4)/k(sub CD4) = 2.5 +/- 0.2. Temperature dependent rate constants for C2H + C2H2 were also remeasured over an increased temperature range from 143 to 359 K and found to show a slight negative temperature dependence, which can be expressed as k(sub C2H2) = 8.6 x 10(exp -16) T(exp 1.8) exp((474 +/- 90)/T) cm(exp 3) molecule(exp -1) s(exp -1).

Quorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: the role of expR(Ecc).

PubMed

Andersson, R A; Eriksson, A R; Heikinheimo, R; Mäe, A; Pirhonen, M; Kõiv, V; Hyytiäinen, H; Tuikkala, A; Palva, E T

2000-04-01

The production of the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the diffusible signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (OHHL). OHHL is synthesized by the product of the expI/carI gene. Linked to expI we found a gene encoding a putative transcriptional regulator of the LuxR-family. This gene, expR(Ecc), is transcribed convergently to the expI gene and the two open reading frames are partially overlapping. The ExpR(Ecc) protein showed extensive amino acid sequence similarity to the repressor EsaR from Pantoea stewartii subsp. stewartii (formerly Erwinia stewartii subsp. stewartii) and to the ExpR(Ech) protein of Erwinia chrysanthemi. Inactivation of the E. carotovora subsp. carotovora expR(Ecc) gene caused no decrease in virulence or production of virulence determinants in vitro. In contrast, there was a slight increase in the maceration capacity of the mutant strain. The effects of ExpR(Ecc) were probably mediated by changes in OHHL levels. Inactivation of expR(Ecc) resulted in increased OHHL levels during early logarithmic growth. In addition, overexpression of expR(Ecc) caused a clear decrease in the production of virulence determinants and part of this effect was likely to be caused by OHHL binding to ExpR(Ecc). ExpR(Ecc) did not appear to exhibit transcriptional regulation of expI, but the effect on OHHL was apparently due to other mechanisms.

Development of microchannel plates in advanced wind-tunnel instrumentation

NASA Technical Reports Server (NTRS)

Feller, W. Bruce

1990-01-01

Microchannel plate (MCP) electron multiplier dynamic range has been increased 3 to 4 orders of magnitude at ambient temperatures, through enhanced input count rate capability and reduced background or 'dark' noise. The previous upper limit of roughly 10(exp 7) - 10(exp 8) cm(exp -2)s(exp -1) at ambient has been extended to levels approach 10(exp 10) cm(exp -2)s(exp -1) under continuous dc operation. The lower limit, previously set by an irreducible background component (approximately 0.6 cm(exp -2)s(exp -1)), has been lowered to the cosmic ray limit of .01 cm(exp -2)s(exp -1). The high end improvement was achieved by conductively cooling a very low resistance MCP by bonding it to a heat sink, while maintaining pulse-counting operation with multianode readouts. The low-end improvement was achieved by removing all radioisotopes from the MCP matrix glass. The detectors will benefit optical and mass spectrometry, flow visualization, plasma diagnostics, magnetometry, and other high signal flux applications. Very low MCP background noise will benefit X-ray and UV astronomy, medical imaging, trace isotope mass spectrometry, and other applications where the signal flux is often extremely low.

Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora.

PubMed

Eriksson, A R; Andersson, R A; Pirhonen, M; Palva, E T

1998-08-01

Production of extracellular, plant cell wall degrading enzymes, the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, is coordinately controlled by a complex regulatory network. Insertion mutants in the exp (extracellular enzyme production) loci exhibit pleiotropic defects in virulence and the growth-phase-dependent transcriptional activation of genes encoding extracellular enzymes. Two new exp mutations, designated expA and expS, were characterized. Introduction of the corresponding wild-type alleles to the mutants complemented both the lack of virulence and the impaired production of plant cell wall degrading enzymes. The expA gene was shown to encode a 24-kDa polypeptide that is structurally and functionally related to the uvrY gene product of Escherichia coli and the GacA response regulator of Pseudomonas fluorescens. Functional similarity of expA and uvrY was demonstrated by genetic complementation. The expA gene is organized in an operon together with a uvrC-like gene, identical to the organization of uvrY and uvrC in E. coli. The unlinked expS gene encodes a putative sensor kinase that shows 92% identity to the recently described rpfA gene product from another E. carotovora subsp. carotovora strain. Our data suggest that ExpS and ExpA are members of two-component sensor kinase and response regulator families, respectively. These two proteins might interact in controlling virulence gene expression in E. carotovora subsp. carotovora.

Aerosol Abundances and Optical Characteristics in the Pacific Basin Free Troposphere

NASA Technical Reports Server (NTRS)

Pueschel, R. F.; Livingston, J. M.; Ferry, G. V.; deFelice, T. E.

1994-01-01

During NASA's Global Backscatter Experiment (GLOBE) mission flights in November 1989 and May 1990, a DC-8 research aircraft probed the Pacific Basin free troposphere for about 90 flight hours in each month between +72 and -62 degrees latitude, +130 and -120 degrees longitude, and up to 39,000 feet pressure altitudes. Aerosols were sampled continuously in situ by optical particle counters to measure concentration and particle size, and during 48 10-min intervals during each mission by wire impactors for concentration, size, composition, phase and shape analyses. The optical particle counters cover a particle diameter range between 0.3 and 20 microns; wire impactors extend the range down to 0.03 microns. Results of particle number, size, shape, together with the assumption of a refractive index corresponding to (NH4)2SO4 to account for the prevalence of aerosol sulfur, were utilized in a Mie algorithm to calculate aerosol extinction and backscatter for a range of wavelengths (0.385 less than lambda less than 10.64 microns). Computations for 22 randomly selected size distributions yield coefficients of extinction E(0.525) = (2.03 +/- 1.20) x 10(exp -4) km(exp -1) and backscatter beta(0.525) = (6.45 +/- 3.49) x 10(exp -6) km(exp -1) sr(exp -1) in the visible, and E(10.64) = (8.13 +/- 6.47) x 10(exp -6) km(exp -1) and beta(10.64) = (9.98 +/- 10.69) x 10(exp -8) km(exp -1) sr(exp -1) in the infrared, respectively. Large particles (D greater than 0.3 microns) contribute two-thirds to the total extinction in the visible (lambda = 0.525 microns), and almost 100% in the infrared (lambda = 10.64 microns). These results have been used to define an IR optical aerosol climatology of the Pacific Basin free troposphere, from which it follows that the infrared backscatter coefficient at lambda = 9.25 microns wavelength fluctuates between 5.0 x 10(exp -10) and 2.0 x 10(exp -7) km(exp -1) sr(exp -1) with a modal value 2.0 x 10(exp -8) km(exp -1) sr(exp -1).

Phylogeographic patterns in New Zealand and temperate Australian cantharidines (Mollusca: Gastropoda: Trochidae: Cantharidinae): Trans-Tasman divergences are ancient.

PubMed

Donald, Kirsten M; Spencer, Hamish G

2016-07-01

Current taxonomic treatments of New Zealand and temperate Australian members of the gastropod subfamily Cantharidinae imply that species on either side of the Tasman Sea are closely related and, in some cases, congeneric. Such a close relationship, however, entails a relatively recent divergence of Australian and New Zealand lineages, which seems inconsistent with what is known about cantharidine larval development in general. In order to address these issues, mitochondrial and nuclear DNA sequences were used to ascertain how cantharidine genera became established over the wide geographical range of temperate Australia and New Zealand, including their subantarctic islands. Our robust and dated phylogenies (based on 16S, COI, 12S and 28S sequences) revealed that Australian and New Zealand species fall into endemic clades that have been separated for, at most, 35million years. This divergence date postdates a vicariant split by around 50million years and we suggest that, once again, long-distance trans-Tasman dispersal has played a pivotal role in molluscan evolution in this part of the world. Our results also show that the current classification requires revision. We recognize three genera (Cantharidus [comprising 2 subgenera: Cantharidus s.str. and Pseudomargarella n. subgen.], Micrelenchus [comprising 2 subgenera: Micrelenchus s.str. and Mawhero] and Roseaplagis n. gen.) for New Zealand cantharidine species. In our dated BEAST tree, these genera form a clade with the endemic Australian Prothalotia and South African Oxystele. Other temperate Australian cantharidines in our study fall into previously recognized genera (Phasianotrochus, Thalotia, Calthalotia), which are all quite distinct from Cantharidus in spite of some authors considering various of them to be possible synonyms. Finally, we remove the Australian genus Cantharidella from the Cantharidinae to the subfamily Trochinae and erect a new genus, Cratidentium n. gen., also in the Trochinae, to accommodate several Australian species previously considered to belong to Cantharidella. Copyright © 2016 Elsevier Inc. All rights reserved.

Shock Tube and Modeling Study of the H + O2 = OH + O Reaction over a Wide Range of Composition, Pressure, and Temperature

NASA Technical Reports Server (NTRS)

Ryu, Si-Ok; Hwang, Soon Muk; Rabinowitz, Martin Jay

1995-01-01

The rate coefficient of the reaction H + 02 = OH + 0 was determined using OH laser absorption spectroscopy behind reflected shock waves over the temperature range 1050-2500 K and the pressure range 0.7-4.0 atm. Eight mixtures and three stoichiometries were used. Two distinct and independent criteria were employed in the evaluation of k(sub 1). Our recommended expression for k(sub 1) is k(sub 1) = 7.13 x 10(exp 13)exp(-6957 K/T) cm(exp 3)mol(exp -1)s(exp -1) with a statistical uncertainty of 6%. A critical review of recent evaluations of k(sub 1) yields a consensus expression given by k(sub 1) = 7.82 x 10(exp 13)exp(-7105 K/7) cm(exp 3)mol(exp -1)s(exp -1) over the temperature range 960-5300 K. We do not support a non-Arrhenius rate coefficient expression, nor do we find evidence of composition dependence upon the determination of k(sub 1).

User ratings of prosthetic usability and satisfaction in VA study to optimize DEKA arm.

PubMed

Resnik, Linda; Borgia, Matthew

2014-01-01

The Department of Veterans Affairs study to optimize the DEKA Arm provided feedback to inform optimization of the gen 2 (second-generation) prototype and evaluate the gen 3 (third-generation) prototype. This article summarizes recommendations to improve gen 2 and reports satisfaction and usability ratings of gen 2 and gen 3. Data were collected from 39 subjects; 37 subjects were included in this analysis. Of the subjects, 24 were fit with gen 2 (8 radial configuration [RC], 6 humeral configuration [HC], and 10 shoulder configuration [SC]), 13 were fit with gen 3 (4 RC, 5 HC, and 4 SC), and 5 were fit with both. Usability and satisfaction were evaluated using the Trinity Amputation and Prosthesis Experience Scale (TAPES) and study-specific usability and satisfaction scales. Descriptive statistics were examined and prototypes compared using Wilcoxon rank-sum. Results were stratified by configuration level and outcomes compared by prototype. Satisfaction and usability were greater for gen 3 than gen 2. Overall TAPES scores were similar; however, scores of the TAPES aesthetic satisfaction subscale were higher for gen 3. Compared with gen 2 users, gen 3 users were more satisfied with appearance, grips, and doffing and rated overall usability higher. Features of gen 3, including weight, external cables and wires, hand covering, and fingernails, would benefit from further optimization.

Thermochemistry of Calcium-Magnesium-Aluminum-Silicate (CMAS) and Components of Advanced Thermal and Environmental Barrier Coating Systems

NASA Technical Reports Server (NTRS)

Costa, Gustavo C. C.; Acosta, Waldo A.; Zhu, Dongming; Ghoshal, Anindya

2017-01-01

There is increasing interest in the degradation mechanism studies of thermal and environmental barrier coatings (TEBCs) of gas turbines by molten CaO-MgO-Al(exp. 2)O(exp. 3)-SiO(exp. 2) CMAS). CMAS minerals are usually referred as silicon-containing sand dust and volcano ash materials that are carried by the intake air into gas turbines, e.g. in aircraft engines, and their deposits often react at high temperatures (greater than 1200 degrees C) with the engine turbine coating systems and components. The high temperature reactions causes degradation and accelerated failure of the static and rotating components of the turbine engines. We discuss some results of the reactions between the CMAS and Rare-Earth (RE = Y, Yb, Dy, Gd, Nd and Sm) - oxide stabilized ZrO(exp. 2) or HfO(exp. 2) systems, and the stability of the resulting oxides and silicates. Plasma sprayed hollow tube samples (outside diameter = 4.7 mm, wall thickness = 0.76 mm and = 26 mm height) were half filled with CMAS powder, wrapped and sealed with platinum foil, and heat treated at 1310 degrees C for 5h. Samples were characterized by differential scanning calorimetry (DSC), X-ray diffraction, and cross-section electron microscopy analysis and energy dispersive X-ray spectroscopy. It was found that CMAS penetrated the samples at the grain boundaries and dissolved the TEBC materials to form silicate phases containing the rare-earth elements. Furthermore, it was found that apatite crystalline phases were formed in the samples with total rare-earth content higher than 12 mol% in the reaction zone for the ZrO(exp. 2) system. In general, samples with the nominal compositions (30YSZ), HfO(exp. 2)-7Dy(exp. 2)O(exp. 2) and ZrO(exp. 2)-9.5Y(exp. 2)O(exp. 3)-2.25Gd(exp. 2)O(exp. 3)-2.25Yb(exp. 2)O(exp. 3) exhibited lower reactivity or more resistance to CMAS than the other coating compositions of this work.

[The 24 May, 2009 isolation of the first A/IIV-Moscow/01/2009 (H1N1)swl strain similar to swine A(H1N1) influenza virus from the first Moscow case detected on May 21, 2009, and its deposit in the state collection of viruses (SCV No. 2452 dated May 24, 2009)].

PubMed

L'vov, D K; Burtseva, E I; Prilipov, A G; Bazarova, M V; Kolobukhina, L V; Merkulova, L N; Malyshev, N A; Deriabin, P G; Fediakina, I T; Sadykova, G K; Usachev, E V; Shchelkanov, M Iu; Shevchenko, E S; Trushakova, S V; Ivanova, V T; Beliakova, N V; Oskerko, T A; Aliper, T I

2009-01-01

The paper presents the results of the first isolation of the new influenza virus in Moscow and the Russian Federation, which was similar to the swine A/IIV-Moscow/01/2009(H1N1)swl strain isolated on May 24, 2009 from a Russian arrived in Moscow from the USA on May 19, 2009. The antigenic, biological, and molecular genetic properties of this virus were studied. The virus was isolated on MDCK and chick embryos, the hemagglutination titers being 1:8-1:16 AE; the infectious titers being 6.51g of the tissue cytopathogenic infective dose (TCID50) and 7.01g of the common infective dose (CID50). The virus was sensitive to arbidol, ribavirin, oseltamivir, and resistant to rimantadine. The complete virus genome was sequenced; the data were accepted to the Gen Bank on May 28, 2009 under GQ219584-GQ219590 and GQ202724. The significant gene substitution of neuraminidase Asp for Gly in position 451, which has been undetectable in any other strain published in the Gen Bank by the present time is unique only to A/IIV-Moscow/01/2009 (H1N1)swl. The virus has been deposited in the State Collection of Viruses, D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, under No. 2452 dated May 24, 2009.

Relationship Between LIBS Ablation and Pit Volume for Geologic Samples: Applications for the In Situ Absolute Geochronology

NASA Technical Reports Server (NTRS)

Devismes, Damien; Cohen, Barbara; Miller, J.-S.; Gillot, P.-Y.; Lefevre, J.-C.; Boukari, C.

2014-01-01

These first results demonstrate that LIBS spectra can be an interesting tool to estimate the ablated volume. When the ablated volume is bigger than 9.10(exp 6) cubic micrometers, this method has less than 10% of uncertainties. Far enough to be directly implemented in the KArLE experiment protocol. Nevertheless, depending on the samples and their mean grain size, the difficulty to have homogeneous spectra will increase with the ablated volume. Several K-Ar dating studies based on this approach will be implemented. After that, the results will be shown and discussed.

--No Title--

Science.gov Websites

$offtext $stitle set definition sets G Power Generators /gen1*gen2/ F Fuels /oil,gas/ K Constants in the fuel consumption equations 0 1 2 gen1.oil 1.4609 .15186 .00145 gen1.gas 1.5742 .16310 .001358 gen2.oil 0.8008 .20310 .000916 gen2.gas 0.7266 .22560 .000778; PARAMETER PMAX(G) Maximum power outputs

Interactive effects of betaine and monensin in uninfected and Eimeria acervulina-infected chicks.

PubMed

Matthews, J O; Ward, T L; Southern, L L

1997-07-01

Three experiments (Exp.) were conducted to evaluate the interactive effects of dietary betaine (BET) and monensin (MON) in uninfected or Eimeria acervulina-infected chicks. The treatments were replicated with six (Exp. 1) or five (Exp. 2 and 3) pens of five chicks each. The experimental periods lasted 9 (Exp. 1 and 2) or 10 (Exp. 3) d each and the coccidiosis infections were established on Day 2 (Exp. 1 and 2) or Day 3 (Exp. 3) of the experiment. Average initial weight of the chicks was 101, 73, and 68 g in Exp. 1 to 3, respectively, and the initial age of the chicks was 5 (Exp. 1) or 4 (Exp. 2 and 3) d. A corn-soybean meal basal diet was used in each experiment. In Exp. 1, the effect of dietary BET (0, 0.1, or 0.5%) in uninfected or coccidiosis-infected (COC; 5 x 10(5) sporulated E. acervulina oocysts) chicks was investigated. In Exp. 2, the interactive effects of BET (0 or 0.1%) and MON (0 or 55 ppm) in uninfected or COC chicks were investigated in a 2 x 2 x 2 factorial arrangement of treatments. Experiment 3 was identical to Exp. 2, except the level of MON was 110 rather than 55 ppm. In Exp. 1, 2, and 3, COC reduced (P < 0.01) gain, feed intake (FI), feed efficiency (GF), and plasma carotenoid concentration (CAR) and increased (P < 0.01) lesion score (LS). In Exp. 1, gain and FI were decreased in uninfected chicks fed 0.1% BET but gain and FI were increased in COC chicks fed 0.1% BET (COC x BET quadratic, P < 0.01). Dietary BET linearly increased (P < 0.05) GF. In Exp. 2 and 3, MON increased (P < 0.01) gain, FI, GF, and CAR and decreased (P < 0.01) LS of COC chicks, but MON had no effect in uninfected chicks (COC x MON, P < 0.01). In Exp. 2, GF was increased more in chicks fed both MON and BET than in chicks fed MON (BET x MON, P < 0.06). In Exp. 3, BET increased GF of uninfected chicks fed MON and of COC chicks not fed MON (COC x BET x MON, P < 0.02). Betaine may have an effect on E. acervulina-infected chicks, but there is no conclusive evidence to indicate that the efficacy of MON is improved when fed in combination with BET.

--No Title--

Science.gov Websites

$stitle set definition sets G Power Generators /gen1*gen2/ F Fuels /oil,gas/ K Constants in Fuel better TABLE A(G,F,K) Coefficients in the fuel consumption equations 0 1 gen1.oil 1.4609 .18223 gen1.gas 1.5742 .19572 gen2.oil 0.8008 .24372 gen2.gas 0.7266 .27072; PARAMETER PMAX(G) Maximum power outputs of

Next-Generation Angular Distribution Models for Top-of-Atmosphere Radiative Flux Calculation from CERES Instruments: Validation

NASA Technical Reports Server (NTRS)

Su, W.; Corbett, J.; Eitzen, Z.; Liang, L.

2015-01-01

Radiative fluxes at the top of the atmosphere (TOA) from the Clouds and the Earth's Radiant Energy System (CERES) instrument are fundamental variables for understanding the Earth's energy balance and how it changes with time. TOA radiative fluxes are derived from the CERES radiance measurements using empirical angular distribution models (ADMs). This paper evaluates the accuracy of CERES TOA fluxes using direct integration and flux consistency tests. Direct integration tests show that the overall bias in regional monthly mean TOA shortwave (SW) flux is less than 0.2Wm(exp -2) and the RMSE is less than 1.1Wm(exp -2). The bias and RMSE are very similar between Terra and Aqua. The bias in regional monthly mean TOA LW fluxes is less than 0.5Wm(exp -2) and the RMSE is less than 0.8Wm(exp -)2 for both Terra and Aqua. The accuracy of the TOA instantaneous flux is assessed by performing tests using fluxes inverted from nadir- and oblique-viewing angles using CERES along-track observations and temporally and spatially matched MODIS observations, and using fluxes inverted from multi-angle MISR observations. The averaged TOA instantaneous SW flux uncertainties from these two tests are about 2.3% (1.9Wm(exp -2) over clear ocean, 1.6% (4.5Wm(exp -2) over clear land, and 2.0% (6.0Wm(exp -) over clear snow/ice; and are about 3.3% (9.0Wm(exp -2), 2.7% (8.4Wm(exp -2), and 3.7% (9.9Wm(exp -2) over ocean, land, and snow/ice under all-sky conditions. The TOA SW flux uncertainties are generally larger for thin broken clouds than for moderate and thick overcast clouds. The TOA instantaneous daytime LW flux uncertainties derived from the CERESMODIS test are 0.5% (1.5Wm(exp -2), 0.8% (2.4Wm(exp -2), and 0.7% (1.3Wm(exp -2) over clear ocean, land, and snow/ice; and are about 1.5% (3.5Wm(exp -2), 1.0% (2.9Wm(exp -2), and 1.1% (2.1Wm(exp -2) over ocean, land, and snow/ice under all-sky conditions. The TOA instantaneous nighttime LW flux uncertainties are about 0.5-1% (<2.0Wm(exp -2) for all surface types. Flux uncertainties caused by errors in scene identification are also assessed by using the collocated CALIPSO, CloudSat, CERES and MODIS data product. Errors in scene identification tend to underestimate TOA SW flux by about 0.6Wm(exp -2) and overestimate TOA daytime (nighttime) LW flux by 0.4 (0.2)Wm(exp -2) when all CERES viewing angles are considered.

Lifetime genistein intake increases the response of mammary tumors to tamoxifen in rats

PubMed Central

Zhang, Xiyuan; Cook, Katherine L; Warri, Anni; Cruz, Idalia M; Rosim, Mariana; Riskin, Jeffrey; Helferich, William; Doerge, Daniel; Clarke, Robert; Hilakivi-Clarke, Leena

2016-01-01

Purpose Whether it is safe for estrogen receptor positive (ER+) breast cancer patients to consume soy isoflavone genistein (GEN) remains controversial. We compared the effects of GEN intake mimicking either Asian (lifetime) or Caucasian (adulthood) intake patterns to that of starting its intake during tamoxifen (TAM) therapy using a preclinical model. Experimental Design Female Sprague-Dawley rats were fed an AIN93G diet supplemented with 0 (control diet) or 500 ppm GEN from postnatal day 15 onwards (lifetime GEN). Mammary tumors were induced with 7,12-dimethylbenz(a)anthracene (DMBA), after which a group of control diet fed rats were switched to GEN diet (adult GEN). When the first tumor in a rat reached 1.4 cm in diameter, TAM was added to the diet, and a subset of previously only control diet fed rats also started GEN intake (post-diagnosis GEN). Results Lifetime GEN intake reduced de novo resistance to TAM, compared with post-diagnosis GEN groups. Risk of recurrence was lower both in the lifetime and adult GEN groups than in the post-diagnosis GEN group. We observed downregulation of unfolded protein response (UPR) and autophagy related genes (GRP78, IRE1α, ATF4 and Beclin-1), and genes linked to immunosuppression (TGFβ and Foxp3), and upregulation of cytotoxic T cell marker CD8a in the tumors of the lifetime GEN group, compared with controls, post-diagnosis, and/or adult GEN groups. Conclusions GEN intake mimicking Asian consumption patterns improved response of mammary tumors to TAM therapy, and this effect was linked to reduced activity of UPR and pro-survival autophagy signaling, and increased anti-tumor immunity. PMID:28148690

Far-Ultraviolet Observations of Comet C 2012 S1 (ISON) From Fortis

NASA Technical Reports Server (NTRS)

Mccandiss, Stephan R.; Feldman, Paul D.; Weaver, Harold; Fleming, Brian; Redwine, Keith; Li, Mary J.; Kutyrev, Alexander; Moseley, S. Harvey

2016-01-01

We have used the unique far-UV imaging capability offered by a sounding-rocket-borne instrument to acquire observations of C/2012 S1 (ISON) when its angular separation with respect to the Sun was 26 deg.3 on 2013 November 20.49. At the time of observation, the comet's heliocentric distance and velocity relative to the Sun were r h = 0.43 au and r (sub h) = 62.7 km s)exp. -1). Images dominated by C Iota Lambda 1657 and H Iota Lambda 1216 were acquired over a 10(exp. 6) x 10(exp. 6) km(exp. 2) region. The water production rate implied by the Ly Alpha observations is constrained to be Q H2O is much greater than 8 x 10(exp. 29) s(exp. -1) while the neutral carbon production rate was Q(sub c) is much greater than 4 x 10(exp. 28) s(exp. -1). The radial profile of C I was consistent with it being a dissociation product of a parent molecule with a lifetime T is approx. 5 x 10(exp. 4) s, favoring a parent other than CO. We constrain the Q(sub co) production rate to 5(+1.5)(-7.5), 10(exp. 28) s(exp. -1) with 1 sigma errors derived from photon statistics. The upper limit on the Q (sub CO)/Q(sub H2O) is 6%.

Force majeure: Will climate change affect our ability to attain Good Environmental Status for marine biodiversity?

PubMed

Elliott, Michael; Borja, Ángel; McQuatters-Gollop, Abigail; Mazik, Krysia; Birchenough, Silvana; Andersen, Jesper H; Painting, Suzanne; Peck, Myron

2015-06-15

The EU Marine Strategy Framework Directive (MSFD) requires that Good Environmental Status (GEnS), is achieved for European seas by 2020. These may deviate from GEnS, its 11 Descriptors, targets and baselines, due to endogenic managed pressures (from activities within an area) and externally due to exogenic unmanaged pressures (e.g. climate change). Conceptual models detail the likely or perceived changes expected on marine biodiversity and GEnS Descriptors in the light of climate change. We emphasise that marine management has to accommodate 'shifting baselines' caused by climate change particularly during GEnS monitoring, assessment and management and 'unbounded boundaries' given the migration and dispersal of highly-mobile species. We suggest climate change may prevent GEnS being met, but Member States may rebut legal challenges by claiming that this is outside its control, force majeure or due to 'natural causes' (Article 14 of the MSFD). The analysis is relevant to management of other global seas. Copyright © 2015 Elsevier Ltd. All rights reserved.

Summary of CPAS Gen II Parachute Analysis

NASA Technical Reports Server (NTRS)

Morris, Aaron L.; Bledsoe, Kristin J.; Fraire, Usbaldo, Jr.; Moore, James W.; Olson, Leah M.; Ray, Eric

2011-01-01

The Orion spacecraft is currently under development by NASA and Lockheed Martin. Like Apollo, Orion will use a series of parachutes to slow its descent and splashdown safely. The Orion parachute system, known as the CEV Parachute Assembly System (CPAS), is being designed by NASA, the Engineering and Science Contract Group (ESCG), and Airborne Systems. The first generation (Gen I) of CPAS testing consisted of thirteen tests and was executed in the 2007-2008 timeframe. The Gen I tests provided an initial understanding of the CPAS parachutes. Knowledge gained from Gen I testing was used to plan the second generation of testing (Gen II). Gen II consisted of six tests: three singleparachute tests, designated as Main Development Tests, and three Cluster Development Tests. Gen II required a more thorough investigation into parachute performance than Gen I. Higher fidelity instrumentation, enhanced analysis methods and tools, and advanced test techniques were developed. The results of the Gen II test series are being incorporated into the CPAS design. Further testing and refinement of the design and model of parachute performance will occur during the upcoming third generation of testing (Gen III). This paper will provide an overview of the developments in CPAS analysis following the end of Gen I, including descriptions of new tools and techniques as well as overviews of the Gen II tests.

Rate Coefficients of C2H with C2H4, C2H6, and H2 from 150 to 359 K

NASA Technical Reports Server (NTRS)

Opansky, Brian J.; Leone, Stephen R.

1996-01-01

Rate coefficients for the reactions C2H with C2H4, C2H6, and H2 are measured over the temperature range 150-359 K using transient infrared laser absorption spectroscopy. The ethynyl radical is formed by photolysis of C2H2 with a pulsed excimer laser at 193 nm, and its transient absorption is monitored with a color center laser on the Q(sub 11)(9) line of the A(sup 2) Pi-Chi(sup 2) Sigma transition at 3593.68 cm(exp -1). Over the experimental temperature range 150-359 K the rate constants of C2H with C2H4, C2H6, and H2 can be fitted to the Arrhenius expressions k(sub C2H4) = (7.8 +/- 0.6) x 10(exp -11) exp[(134 +/- 44)/T], k(sub C2H6) = (3.5 +/- 0.3) x 10(exp -11) exp[(2.9 +/- 16)/T], and k(sub H2) = (1.2 +/- 0.3) x 10(exp -11) exp[(-998 +/- 57)]/T cm(exp 3) molecule(exp -1) sec(exp -1). The data for C2H with C2H4 and C2H6 indicate a negligible activation energy to product formation shown by the mild negative temperature dependence of both reactions. When the H2 data are plotted together with the most recent high-temperature results from 295 to 854 K, a slight curvature is observed. The H2 data can be fit to the non-Arrhenius form k(sub H2) = 9.2 x 10(exp -18) T(sup 2.17 +/- 0.50) exp[(-478 +/- 165)/T] cm(exp 3) molecules(exp -1) sec(exp -1). The curvature in the Arrhenius plot is discussed in terms of both quantum mechanical tunneling of the H atom from H2 to the C2H radical and bending mode contributions to the partition function.

GRBs: The Most Distant Signposts in our Universe

NASA Technical Reports Server (NTRS)

Kouveliotou, Chryssa

2007-01-01

Gamma-Ray Bursts (GRBs) are the most powerful photon sources in the Universe, rivaled only by supernovae in the magnitude of their energy release. In 1997 GRB were found to originate in host galaxies at cosmological distances, revealing the total energy of their explosions to be an astounding approx.10(exp 52) - 10(exp 53)ergs. GRB durations span over five orders of magnitude, ranging from milliseconds to thousands of seconds. The underlying sources of the energy release remain, however, unknown. Leading candidates are mergers, either of two neutron stars or of a black hole and a neutron star, and core collapses of very massive stars, called "collapsars". To date the furthest GRB galaxy has been found at a cosmological redshift of 6.29, very close to the most distant quasar (at z=6.4). Since the Swift satellite continues to observe these phenomena at a rate of approx.120 per year, and with the upcoming launch of GLAST with two burst instruments on board, we will be able to use GRBs as beacons to probe very high redshifts. Thus bursts found at 6

Vector and Axial Form Factors Applied to Neutrino Quasielastic Scattering

NASA Astrophysics Data System (ADS)

Budd, H.; Bodek, A.; Arrington, J.

2005-02-01

We calculate the quasielastic cross sections for neutrino scattering on nucleons using up to date fits to the nucleon elastic electromagnetic form factors GEp, GEn, GMp, GMn, and weak form factors. We show the extraction of F(q). We show the that F(q) has a different contribution to the anti-neutrino cross section, and how the anti-neutrino data can be used to check F(q) extracted from neutrino scattering. (Presented by Howard Budd at NuInt04, Mar. 2004, Laboratori Nazionali del Gran Sasso - INFN - Assergi, Italy [ http://nuint04.lngs.infn.it/])

Tests of the Rockwell Si:As Back-Illuminated Blocked-Impurity Band (BIBIB) detectors

NASA Technical Reports Server (NTRS)

Wolf, J.; Groezinger, U.; Burgdorf, M.; Salama, A.

1989-01-01

Two arrays of Rockwell's Si:As back-illuminated blocked-impurity-band detectors were tested at the Max-Planck-Institute for Astronomy (MPIA) at low background and low temperature for possible use in the astronomical space experiment ISOPHOT. For these measurements special test equipment was put together. A cryostat was mechanically modified to accommodate the arrays and special peripheral electronics was added to a microprocessor system to drive the cold multiplexer and to acquire the output data. The first device, a 16x50 element array on a fan-out board was used to test individual pixels with a trans-impedance-amplifier at a photon background of 10(exp 8) Ph s(-1)cm(-2) and at temperatures of 2.7 to 4.4 K. The noise-equivalent-power NEP is in the range 5 - 7 x 10(exp -18) WHz(exp -1/2), the responsivity is less than or equal to 100 AW(exp -1)(f = 10 Hz). The second device was a 10x50 array including a cold readout electronics of switched FETs (SWIFET). Measurements of this array were done in a background range of 5 x 10(exp 5) to 5 x 10(exp 11) Ph s(exp-1)cm(exp-2) and at operating temperatures between 3.0 and 4.8 K. The NEP ranges from less than 10(exp -18) at the lowest background to 2 x 10(exp -16) WHz(exp -1/2) at the highest flux.

The Battle of Aschaffenburg: An Example of Late World War 2 Urban Combat in Europe

DTIC Science & Technology

1989-06-02

Division (LTC Abams) German Units - ARMY: Seventh ( Gen . der Inf. Felber) ( Gen . der Inf. Obstfelder, 26 March) CORPS: L)XOII ( Gen . dir Inf. Hahn...4 Armored Division (LTC Abrams) Ist Bn, 104 Infantry (attached to 4 AD) German Units - ARMY: Seventh ( Gen . dir Inf. Obstfelder) CORPS: DOCOII ( Gen ...Haislip) DIVISION: 44 Infantry Division (MG Dean) 45 Infantry Division (MG Fredericks) German Units - ARMY: Seventh ( Gen . dir Inf. Obstfelder) CORPS

Relationship Between Ecosystem Productivity and Photosynthetically Active Radiation for Northern Peatlands

NASA Technical Reports Server (NTRS)

Frolking, S. E.; Bubier, J. L.; Moore, T. R.; Ball, T.; Bellisario, L. M.; Bhardwaj, A.; Carroll, P.; Crill, P. M.; Lafleur, P. M.; McCaughey, J. H.;

Down-regulation of the IbEXP1 gene enhanced storage root development in sweetpotato

PubMed Central

Bae, Jung Myung

2013-01-01

The role of an expansin gene (IbEXP1) in the formation of the storage root (SR) was investigated by expression pattern analysis and characterization of IbEXP1-antisense sweetpotato (Ipomoea batatas cv. Yulmi) plants in an attempt to elucidate the molecular mechanism underlying SR development in sweetpotato. The transcript level of IbEXP1 was high in the fibrous root (FR) and petiole at the FR stage, but decreased significantly at the young storage root (YSR) stage. IbEXP1-antisense plants cultured in vitro produced FRs which were both thicker and shorter than those of wild-type (WT) plants. Elongation growth of the epidermal cells was significantly reduced, and metaxylem and cambium cell proliferation was markedly enhanced in the FRs of IbEXP1-antisense plants, resulting in an earlier thickening growth in these plants relative to WT plants. There was a marked reduction in the lignification of the central stele of the FRs of the IbEXP1-antisense plants, suggesting that the FRs of the mutant plants possessed a higher potential than those of WT plants to develop into SRs. IbEXP1-antisense plants cultured in soil produced a larger number of SRs and, consequently, total SR weight per IbEXP1-antisense plant was greater than that per WT plant. These results demonstrate that SR development was accelerated in IbEXP1-antisense plants and suggest that IbEXP1 plays a negative role in the formation of SR by suppressing the proliferation of metaxylem and cambium cells to inhibit the initial thickening growth of SRs. IbEXP1 is the first sweetpotato gene whose role in SR development has been directly identified in soil-grown transgenic sweetpotato plants. PMID:22945944

Comparative Studies for the Sodium and Potassium Atmospheres of the Moon and Mercury

NASA Technical Reports Server (NTRS)

Smyth, William H.

1999-01-01

A summary discussion of recent sodium and potassium observations for the atmospheres of the Moon and Mercury is presented with primary emphasis on new full-disk images that have become available for sodium. For the sodium atmosphere, image observations for both the Moon and Mercury are fitted with model calculations (1) that have the same source speed distribution, one recently measured for electron-stimulated desorption and thought to apply equally well to photon-stimulated desorption, (2) that have similar average surface sodium fluxes, about 2.8 x 10(exp 5) to 8.9 x 10(exp 5) atoms cm(exp -2)s(exp -1) for the Moon and approximately 3.5 x 10(exp 5) to 1.4 x 10(exp 6) atoms cm(exp -2)s(exp -1) for Mercury, but (3) that have very different distributions for the source surface area. For the Moon, a sunlit hemispherical surface source of between approximately 5.3 x 10(exp 22) to 1.2 x 10(exp 23) atoms/s is required with a spatial dependence at least as sharp as the square of the cosine of the solar zenith angle. For Mercury, a time dependent source that varies from 1.5 x 10(exp 22) to 5.8 x l0(exp 22) atoms/s is required which is confined to a small surface area located at, but asymmetrically distributed about, the subsolar point. The nature of the Mercury source suggest that the planetary magnetopause near the subsolar point acts as a time varying and partially protective shield through which charged particles may pass to interact with and liberate gas from the planetary surface. Suggested directions for future research activities are discussed.

40 CFR Appendix - Tables to Part 132

Code of Federal Regulations, 2011 CFR

2011-07-01

.... c CMCt=exp {mA[pH]+bA}. The CMCt shall be rounded to two significant digits. Notes: The term “exp...,b 0.846 +2.255 0.998 Pentachlorophenol c 1.005 −4.869 n/a Zinc a,b 0.8473 +0.884 0.978 a CMCtr=exp... CMCt=exp mA {[pH]+bA}. The CMCt shall be rounded to two significant digits. Notes: The term “exp...

Pooled nucleic acid testing increases the diagnostic yield of acute HIV infections in a high-risk population compared to 3rd and 4th generation HIV enzyme immunoassays.

PubMed

Krajden, Mel; Cook, Darrel; Mak, Annie; Chu, Ken; Chahil, Navdeep; Steinberg, Malcolm; Rekart, Michael; Gilbert, Mark

2014-09-01

We compared a 3rd generation (gen) and two 4th gen HIV enzyme immunoassays (EIA) to pooled nucleic acid testing (PNAT) for the identification of pre- and early seroconversion acute HIV infection (AHI). 9550 specimens from males >18 year from clinics attended by men who have sex with men were tested by Siemens ADVIA Centaur(®) HIV 1/O/2 (3rd gen) and HIV Combo (4th gen), as well as by Abbott ARCHITECT(®) HIV Ag/Ab Combo (4th gen). Third gen non-reactive specimens were also tested by Roche COBAS(®) Ampliprep/COBAS® TaqMan HIV-1 Test v.2 in pools of 24 samples. Sensitivity and specificity of the three EIAs for AHI detection were compared. 7348 persons contributed 9435 specimens and had no evidence of HIV infection, 79 (94 specimens) had established HIV infection, 6 (9 specimens) had pre-seroconversion AHI and 9 (12 specimens) had early seroconversion AHI. Pre-seroconversion AHI cases were not detected by 3rd gen EIA, whereas 2/6 (33.3%) were detected by Siemens 4th gen, 4/6 (66.7%) by Abbott 4th gen and 6/6 (100%) by PNAT. All three EIAs and PNAT detected all individuals with early seroconversion AHI. Overall sensitivity/specificity for the EIAs relative to WB or NAT resolved infection status was 93.6%/99.9% for Siemens 3rd gen, 95.7%/99.7% for Siemens 4th gen and 97.9%/99.2% for Abbott 4th gen. While both 4th gen EIAs demonstrated improved sensitivity for AHI compared to 3rd gen EIA, PNAT identified more AHI cases than either 4th gen assay. PNAT is likely to remain a useful strategy to identify AHI in high-risk populations. Copyright © 2014 Elsevier B.V. All rights reserved.

A comparison of cosmological hydrodynamic codes

NASA Technical Reports Server (NTRS)

Kang, Hyesung; Ostriker, Jeremiah P.; Cen, Renyue; Ryu, Dongsu; Hernquist, Lars; Evrard, August E.; Bryan, Greg L.; Norman, Michael L.

1994-01-01

We present a detailed comparison of the simulation results of various hydrodynamic codes. Starting with identical initial conditions based on the cold dark matter scenario for the growth of structure, with parameters h = 0.5 Omega = Omega(sub b) = 1, and sigma(sub 8) = 1, we integrate from redshift z = 20 to z = O to determine the physical state within a representative volume of size L(exp 3) where L = 64 h(exp -1) Mpc. Five indenpendent codes are compared: three of them Eulerian mesh-based and two variants of the smooth particle hydrodynamics 'SPH' Lagrangian approach. The Eulerian codes were run at N(exp 3) = (32(exp 3), 64(exp 3), 128(exp 3), and 256(exp 3)) cells, the SPH codes at N(exp 3) = 32(exp 3) and 64(exp 3) particles. Results were then rebinned to a 16(exp 3) grid with the exception that the rebinned data should converge, by all techniques, to a common and correct result as N approaches infinity. We find that global averages of various physical quantities do, as expected, tend to converge in the rebinned model, but that uncertainites in even primitive quantities such as (T), (rho(exp 2))(exp 1/2) persists at the 3%-17% level achieve comparable and satisfactory accuracy for comparable computer time in their treatment of the high-density, high-temeprature regions as measured in the rebinned data; the variance among the five codes (at highest resolution) for the mean temperature (as weighted by rho(exp 2) is only 4.5%. Examined at high resolution we suspect that the density resolution is better in the SPH codes and the thermal accuracy in low-density regions better in the Eulerian codes. In the low-density, low-temperature regions the SPH codes have poor accuracy due to statiscal effects, and the Jameson code gives the temperatures which are too high, due to overuse of artificial viscosity in these high Mach number regions. Overall the comparison allows us to better estimate errors; it points to ways of improving this current generation ofhydrodynamic codes and of suiting their use to problems which exploit their best individual features.

Growth of potatoes for CELSS

NASA Technical Reports Server (NTRS)

Tibbitts, T. W.; Cao, W.; Wheeler, R. M.

1994-01-01

This report summarizes research on the utilization of white potatoes (Solanum tuberosum L.) for space life support systems at the University of Wisconsin-Madison over the period of 1984 to 1993. At full maturity the tuber productivity was 37.5 gm(exp -2) d(exp -1), equating to a growing area requirement for one human (2800 kcal d(exp -1)) of 10.1 m(exp -2). A recirculating nutrient system using slanted trays produced best potato growth and tuber yields when a 2-3 cm layer of gravel or arcillite media was utilized. Potato production was close to maximum under lighting levels of 400 micromol m(exp -2) s(exp -1) of photosynthetic photo flux (PPF) for 24 hours or 800 micromol m(exp -2) s(exp -1) for 12 hours, alternating diurnal temperatures of 22 C and 14 C, relative humidity of 85 percent, and a carbon dioxide level of 1000 micromol m(exp -1). The range of effective concentrations of each separate nutrient is reported. The extensive studies with potatoes in this project have demonstrated that this crop has high productivity of nutritous tubers with a high harvest index in controlled environments, and can fulfill a significant portion of the energy and protein requirements for humans in space.

Four new genera and five new species of lecanicephalideans (Cestoda: Lecanicephalidea) from elasmobranchs in the Gulf of California, Mexico.

PubMed

Jensen, K

2001-08-01

A total of 53 spiral intestines from 3 species of rays collected in the Gulf of California, Baja, Mexico, was examined for cestodes of the order Lecanicephalidea. Four new genera and 5 new species were discovered as a result of this study. These are Aberrapex senticosus n. gen., n. sp., from Myliobatis californica, Paraberrapex manifestus n. gen., n. sp., from Squatina californica and Healyum harenamica n. gen., n. sp., Healyum pulvis n. sp. and Quadcuspibothrium francisi n. gen., n. sp., from Mobula japanica. Aberrapex n. gen. and Paraberrapex n. gen. can be distinguished from all other lecanicephalidean genera based on the lack of an apical structure (pars apicalis) on their scolex. Aberrapex n. gen. can be distinguished from Paraberrapex n. gen. based on the presence of an external seminal vesicle, a vagina positioned lateral rather than medial in the proglottid, and an ovary that is tetralobed rather than bilobed in cross section. Healyum n. gen. and Quadcuspibothrium n. gen. are unique among lecanicephalidean genera, including Aberrapex n. gen. and Paraberrapex n. gen., in their possession of a trilobed ovary in cross section. Quadcuspibothrium n. gen. can be distinguished from Healyum n. gen. based on its unique shape of the acetabula, which are diamond-shaped. The 2 species of Healyum n. gen. can be distinguished from one another based on the dimensions and the shape of the scolex, the diameter of the suckerlike acetabula, as well as the shape of the spiniform microtriches on the acetabular rims. Discobothrium arrhynchum is transferred to the genus Aberrapex n. gen. and the original description of this species is emended to include details of the vas deferens and the uterine duct and uterus. The microtrich patterns of the 5 new species and Aberrapex arrhynchum n. comb. are described. These are the first records of tapeworms of the Lecanicephalidea from the Gulf of California. Squatina californica and Mobula japanica represent new host records for lecanicephalidean tapeworms. The ordinal diagnosis of the Lecanicephalidea by Euzet (1994) is emended to include these 4 new genera.

Evaluation bias in objective response rate and disease control rate between blinded independent central review and local assessment: a study-level pooled analysis of phase III randomized control trials in the past seven years.

PubMed

Zhang, Jianrong; Zhang, Yiyin; Tang, Shiyan; Liang, Hengrui; Chen, Difei; Jiang, Long; He, Qihua; Huang, Yu; Wang, Xinyu; Deng, Kexin; Jiang, Shuhan; Zhou, Jiaqing; Xu, Jiaxuan; Chen, Xuanzuo; Liang, Wenhua; He, Jianxing

2017-12-01

In previous studies, complete-case implementation of blind independent central review has been considered unnecessary based on no sign of systematic bias between central and local assessments. In order to further evaluate its value, this study investigated evaluation status between both assessments in phase III trials of anti-cancer drugs for non-hematologic solid tumors. Eligible trials were searched in PubMed with the date of Jan 1, 2010 to Jun 30, 2017. We compared objective response rate (ORR) and disease control rate (DCR) between central and local assessments by study-level pooled analysis and correlation analysis. In pooled analysis, direct comparison was measured by the odds ratio (OR) of central-assessed response status to local-assessed response status; to investigate evaluation bias between central and local assessments, the above calculated OR between experimental (exp-) and control (con-) arms were compared, measured by the ratio of OR. A total of 28 included trials involving 17,466 patients were included (28 with ORR, 16 with DCR). Pooled analysis showed central assessment reported lower ORR and DCR than local assessment, especially in trials with open-label design, central-assessed primary endpoint, and positive primary endpoint outcome, respectively. However, this finding could be found in both experimental [exp-ORR: OR=0.81 (95% CI: 0.76-0.87), P<0.01, I 2 =11%; exp-DCR: OR=0.90 (0.81-1.01), P=0.07, I 2 =42%] and control arms [con-ORR: OR=0.79 (0.72-0.85), P<0.01, I 2 =17%; con-DCR: OR=0.94 (0.86-1.02), P=0.14, I 2 =12%]. No sign of evaluation bias between two assessments was indicated through further analysis [ORR: ratio of OR=1.02 (0.97-1.07), P=0.42, I 2 =0%; DCR: ratio of OR=0.98 (0.93-1.03), P=0.37, I 2 =0%], regardless of mask (open/blind), sample size, tumor type, primary endpoint (central-assessed/local-assessed), and primary endpoint outcome (positive/negative). Correlation analysis demonstrated a high-degree concordance between central and local assessments (exp-ORR, con-ORR, exp-DCR, con-DCR: r>0.90, P<0.01). Blind independent central review remained irreplaceable to monitor local assessment, but its complete-case implementation may be unnecessary.

Ozone Production by Corona Discharges During a Convective Event in DISCOVER-AQ Houston

NASA Technical Reports Server (NTRS)

Kotsakis, Alexander; Morris, Gary A.; Lefer, Barry; Jeon, Wongbae; Roy, Anirban; Minschwaner, Ken; Thompson, Anne M.; Choi, Yunsoo

2017-01-01

An ozonesonde launched near electrically active convection in Houston, TX on 5 September 2013 during the NASA DISCOVER-AQ project measured a large enhancement of ozone throughout the troposphere. A separate ozonesonde was launched from Smith Point, TX (approx. 58 km southeast of the Houston site) at approximately the same time as the launch from Houston and did not measure that enhancement. Furthermore, ozone profiles for the descent of both sondes agreed well with the ascending Smith Point profile, suggesting a highly localized event in both space and time in which an anomalously large enhancement of 70 - 100 ppbv appeared in the ascending Houston ozonesonde data. Compared to literature values, such an enhancement appears to be the largest observed to date. Potential sources of the localized ozone enhancement such as entrainment of urban or biomass burning emissions, downward transport from the stratosphere, photochemical production from lightning NO(sub x), and direct ozone production from corona discharges were investigated using model simulations. We conclude that the most likely explanation for the large ozone enhancement is direct ozone production by corona discharges. Integrating the enhancement seen in the Houston ozone profile and using the number of electrical discharges detected by the NLDN (or HLMA), we estimate a production of 2.48 x 10(exp. 28) molecules of ozone per flash which falls within the range of previously recorded values (9.89 x 10(exp. 26) - 9.82 x 10)exp. 28) molecules of ozone per flash). Since there is currently no parameterization for the direct production of ozone from corona discharges we propose the implementation of an equation into a chemical transport model. Ultimately, additional work is needed to further understand the occurrence and impact of corona discharges on tropospheric chemistry on short and long timescales.

Aboveground Biomass Variability Across Intact and Degraded Forests in the Brazilian Amazon

NASA Technical Reports Server (NTRS)

Longo, Marcos; Keller, Michael; Dos-Santos, Maiza N.; Leitold, Veronika; Pinage, Ekena R.; Baccini, Alessandro; Saatchi, Sassan; Nogueira, Euler M.; Batistella, Mateus; Morton, Douglas C.

2016-01-01

Deforestation rates have declined in the Brazilian Amazon since 2005, yet degradation from logging, re, and fragmentation has continued in frontier forests. In this study we quantified the aboveground carbon density (ACD) in intact and degraded forests using the largest data set of integrated forest inventory plots (n 359) and airborne lidar data (18,000 ha) assembled to date for the Brazilian Amazon. We developed statistical models relating inventory ACD estimates to lidar metrics that explained70 of the variance across forest types. Airborne lidar-ACD estimates for intact forests ranged between 5.0 +/- 2.5 and 31.9 +/- 10.8 kg C m(exp -2). Degradation carbon losses were large and persistent. Sites that burned multiple times within a decade lost up to 15.0 +/- 0.7 kg C m(-2)(94%) of ACD. Forests that burned nearly15 years ago had between 4.1 +/- 0.5 and 6.8 +/- 0.3 kg C m(exp -2) (22-40%) less ACD than intact forests. Even for low-impact logging disturbances, ACD was between 0.7 +/- 0.3 and 4.4 +/- 0.4 kg C m(exp -2)(4-21%) lower than unlogged forests. Comparing biomass estimates from airborne lidar to existing biomass maps, we found that regional and pan-tropical products consistently overestimated ACD in degraded forests, under-estimated ACD in intact forests, and showed little sensitivity to res and logging. Fine-scale heterogeneity in ACD across intact and degraded forests highlights the benefits of airborne lidar for carbon mapping. Differences between airborne lidar and regional biomass maps underscore the need to improve and update biomass estimates for dynamic land use frontiers, to better characterize deforestation and degradation carbon emissions for regional carbon budgets and Reduce Emissions from Deforestation and forest Degradation(REDD+).

Impact Crises, Mass Extinctions, and Galactic Dynamics: A Unified Theory

NASA Technical Reports Server (NTRS)

Rampino, M.R.

1997-01-01

A general hypothesis linking mass extinctions of life with impacts of large asteroids and comets is based on astronomical data, impact dynamics, and geological information. The waiting times of large-body impacts on the Earth, derived from the flux of Earth-crossing asteroids and comets, and the estimated size of impacts capable of causing large-scale environmental disasters predict that impacts of objects (sup 3)5 km in diameter ((sup 3)10(exp 7) Mt TNT equivalent) could be sufficient to explain the record of about 25 extinction pulses in the last 540 m.y., with the five recorded major mass extinctions related to the impacts of the largest objects of (sup 3)10 km in diameter ( (sup 3)10(exp 8) Mt events). Smaller impacts (about 10(exp 6)-10(exp 7) Mt), with significant regional and even global environmental effects, could be responsible for the lesser boundaries in the geologic record. Tests of the "kill curve" relationship for impact-induced extinctions based on new data on extinction intensities and several well-dated large impact craters suggest that major mass extinctions require large impacts, and that a step in the kill curve may exist at impacts that produce craters of -100 km diameter, with smaller impacts capable of only relatively weak extinction pulses. Single impact craters < about 60 km in diameter should not be associated with global extinction pulses detectable in the Sepkoski database (although they may explain stage and zone boundaries marked by lesser faunal turnover), but multiple impacts in that size range may produce significant stepped extinction pulses. Statistical tests of the last occurrences of species at mass-extinction boundaries are generally consistent with predictions for abrupt or stepped extinctions, and several boundaries are known to show "catastrophic" signatures of environmental disasters and biomass crash, impoverished postextinction fauna and flora dominated by stress-tolerant and opportunistic species, and gradual ecological recovery and radiation of new taxa. Isotopic and other geochemical signatures are also generally consistent with the expected after-effects of catastrophic impacts. Seven of the recognized extinction pulses are associated with concurrent (in some cases multiple) stratigraphic impact markers (e.g., layers with high Ir, shocked minerals, microtektites), and/or large, dated impact craters. Other less-well-studied crisis intervals show elevated Ir, still well below that of the K/T spike, which might be explained by low-Ir impactors, ejecta blowoff, or the sedimentary reworking and dilution of impact signatures. The best explanation for a possible periodic component of about 30 m.y. in mass extinctions and clusters of impacts is the modulation of the comet flux associated with the solar system's periodic passage through the plane of the Milky Way Galaxy. The quantitative agreement among paleontological, geological, and astronomical data suggests an important underlying unification of the processes involved.

Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend.

PubMed

Stackebrandt, E; Koch, C; Gvozdiak, O; Schumann, P

1995-10-01

The results of a phylogenetic and chemotaxonomic analysis of the genus Micrococcus indicated that it is significantly heterogeneous. Except for Micrococcus lylae, no species groups phylogenetically with the type species of the genus, Micrococcus luteus. The other members of the genus form three separate phylogenetic lines which on the basis of chemotaxonomic properties can be assigned to four genera. These genera are the genus Kocuria gen. nov. for Micrococcus roseus, Micrococcus varians, and Micrococcus kristinae, described as Kocuria rosea comb. nov., Kocuria varians comb. nov., and Kocuria kristinae comb. nov., respectively; the genus Nesterenkonia gen. nov. for Micrococcus halobius, described as Nesterenkonia halobia comb. nov.; the genus Nesterenkonia gen. nov. for Micrococcus halobius, described as Nesterenkonia halobia comb. nov.; the genus Dermacoccus gen. nov. for Micrococcus nishinomiyaensis, described as Dermacoccus nishinomiyaensis comb. nov.; and the genus Kytocossus gen. nov. for Micrococcus sedentarius, described as Kytococcus sedentarius comb. nov. M. luteus and M. lylae, which are closely related phylogenetically but differ in some chemotaxonomic properties, are the only species that remain in the genus Micrococcus Cohn 1872. An emended description of the genus Micrococcus is given [corrected].

Alteration of renal excretion pathways in gentamicin-induced renal injury in rats.

PubMed

Ma, Yan-Rong; Luo, Xuan; Wu, Yan-Fang; Zhang, Tiffany; Zhang, Fan; Zhang, Guo-Qiang; Wu, Xin-An

2018-07-01

The kidney plays a major part in the elimination of many drugs and their metabolites, and drug-induced kidney injury commonly alters either glomerular filtration or tubular transport, or both. However, the renal excretion pathway of drugs has not been fully elucidated at different stages of renal injury. This study aimed to evaluate the alteration of renal excretion pathways in gentamicin (GEN)-induced renal injury in rats. Results showed that serum cystatin C, creatinine and urea nitrogen levels were greatly increased by the exposure of GEN (100 mg kg -1 ), and creatinine concentration was increased by 39.7% by GEN (50 mg kg -1 ). GEN dose-dependently upregulated the protein expression of rOCT1, downregulated rOCT2 and rOAT1, but not affected rOAT2. Efflux transporters, rMRP2, rMRP4 and rBCRP expressions were significantly increased by GEN(100), and the rMATE1 level was markedly increased by GEN(50) but decreased by GEN(100). GEN(50) did not alter the urinary excretion of inulin, but increased metformin and furosemide excretion. However, GEN(100) resulted in a significant decrease of the urinary excretion of inulin, metformin and p-aminohippurate. In addition, urinary metformin excretions in vivo were significantly decreased by GEN(100), but slightly increased by GEN(50). These results suggested that GEN(50) resulted in the induction of rOCTs-rMATE1 and rOAT3-rMRPs pathway, but not changed the glomerular filtration rate, and GEN(100)-induced acute kidney injury caused the downregulated function of glomerular filtration -rOCTs-rMATE1 and -rOAT1-rMRPs pathway. Copyright © 2018 John Wiley & Sons, Ltd.

DOE/NNSA perspective safeguard by design: GEN III/III+ light water reactors and beyond

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pan, Paul Y

2010-12-10

An overview of key issues relevant to safeguards by design (SBD) for GEN III/IV nuclear reactors is provided. Lessons learned from construction of typical GEN III+ water reactors with respect to SBD are highlighted. Details of SBD for safeguards guidance development for GEN III/III+ light water reactors are developed and reported. This paper also identifies technical challenges to extend SBD including proliferation resistance methodologies to other GEN III/III+ reactors (except HWRs) and GEN IV reactors because of their immaturity in designs.

Superconducting techniques for gravity survey and inertial navigation

NASA Technical Reports Server (NTRS)

Chan, H. A.; Moody, M. V.; Paik, H. J.; Parke, J. W.

1985-01-01

A three-axis gravity gradiometer is developed, in which the magnetic fields produced by persistent currents are modulated by motions of superconducting proof masses. The common-acceleration-induced errors are compensated for by a six-axis superconducting accelerometer with a single magnetically levitated proof mass, linear acceleration resolution of 4 x 10 to the -12th m/(s exp 2 Hz exp 1/2), and angular acceleration resolution of 3 x 10 to the -11th rad/(s exp 2 Hz exp 1/2). The testing of a prototype gradiometer revealed that the environment-induced noise limits the noise floor to 7 x 10 to the -10th/(s exp 2 Hz exp 1/2).

Comparative sequence analyses on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov

NASA Technical Reports Server (NTRS)

Wisotzkey, J. D.; Jurtshuk, P. Jr; Fox, G. E.; Deinhard, G.; Poralla, K.

1992-01-01

Comparative 16S rRNA (rDNA) sequence analyses performed on the thermophilic Bacillus species Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus revealed that these organisms are sufficiently different from the traditional Bacillus species to warrant reclassification in a new genus, Alicyclobacillus gen. nov. An analysis of 16S rRNA sequences established that these three thermoacidophiles cluster in a group that differs markedly from both the obligately thermophilic organisms Bacillus stearothermophilus and the facultatively thermophilic organism Bacillus coagulans, as well as many other common mesophilic and thermophilic Bacillus species. The thermoacidophilic Bacillus species B. acidocaldarius, B. acidoterrestris, and B. cycloheptanicus also are unique in that they possess omega-alicylic fatty acid as the major natural membranous lipid component, which is a rare phenotype that has not been found in any other Bacillus species characterized to date. This phenotype, along with the 16S rRNA sequence data, suggests that these thermoacidophiles are biochemically and genetically unique and supports the proposal that they should be reclassified in the new genus Alicyclobacillus.

Initial astronomical results with a new 5-14 micron Si:Ga 58x62 DRO array camera

NASA Technical Reports Server (NTRS)

Gezari, Dan; Folz, Walter; Woods, Larry

1989-01-01

A new array camera system was developed using a 58 x 62 pixel Si:Ga (gallium doped silicon) DRO (direct readout) photoconductor array detector manufactured by Hughes/Santa Barbara Research Center (SBRC). The camera system is a broad band photometer designed for 5 to 14 micron imaging with large ground-based optical telescopes. In a typical application a 10 micron photon flux of about 10(exp 9) photons sec(exp -1) m(exp -2) microns(exp -1) arcsec(exp -2) is incident in the telescope focal plane, while the detector well capacity of these arrays is 10(exp 5) to 10 (exp 6) electrons. However, when the real efficiencies and operating conditions are accounted for, the 2-channel 3596 pixel array operates with about 1/2 full wells at 10 micron and 10% bandwidth with high duty cycle and no real experimental compromises.

Meteoroid Flux from Lunar Impact Monitoring

NASA Technical Reports Server (NTRS)

Suggs, Robert; Moser, Danielle; Cooke, William; Suggs, Ronnie

2015-01-01

The flux of kilogram-sized meteoroids has been determined from the first 5 years of observations by NASA's Lunar Impact Monitoring Program (Suggs et al. 2014). Telescopic video observations of 126 impact flashes observed during photometric conditions were calibrated and the flux of meteoroids to a limiting mass of 30 g was determined to be 6.14 x 10(exp -10) m(exp -2) yr(exp -1) at the Moon, in agreement with the Grun et al. (1985) model value of 7.5 x 10(exp -10) m(exp -2) yr(exp -1). After accounting for gravitational focusing effects, the flux at the Earth to a limiting impact energy of 3.0 x10(exp -6) kilotons of TNT (1.3 x 10(exp 7) J) was determined to be consistent with the results in Brown et al. (2002). Approximately 62% of the impact flashes were correlated with major meteor showers as cataloged in visual/optical meteor shower databases. These flux measurements, coupled with cratering and ejecta models, can be used to develop impact ejecta engineering environments for use in lunar surface spacecraft design and risk analyses.

PSR J2021+4026 in the Gamma Cygni Region: The First Variable Gamma-Ray Pulsar Seen by the Fermi LAT

NASA Technical Reports Server (NTRS)

Allafort, A.; Baldini, L.; Ballet, J.; Barbiellini, G.; Baring, M. G.; Bastieri, D.; Bellazzini, R.; Bonamente, E.; Bottacini, E.; Brandt, T. J.;

Submaximal doses of ghrelin do not inhibit gonadotrophin levels but stimulate prolactin secretion in postmenopausal women.

PubMed

Messini, Christina I; Malandri, Maria; Anifandis, George; Dafopoulos, Konstantinos; Georgoulias, Panagiotis; Sveronis, Georgios; Garas, Antonios; Daponte, Alexandros; Messinis, Ioannis E

2017-07-01

An inhibitory effect of ghrelin on gonadotrophin secretion has been reported in normally menstruating women possibly modulated by endogenous oestrogen. The aim of this study was to examine the effect of ghrelin on gonadotrophin and prolactin (PRL) secretion in oestrogen-deprived postmenopausal women. Prospective intervention study. Ten healthy postmenopausal volunteer women were studied during two 15-days periods of oestrogen treatment (A and B) a month apart. Four experiments (Exp) were performed in total, two on day 1 (Exp 1A and Exp 1B) and two on day 15 (Exp 15A and Exp 15B) of the two periods. The women received in Exp 1A and in Exp 15A two iv injections of ghrelin (0.15 μg/kg at time 0 minute and 0.30 μg/kg at time 90 minutes) and in Exp1B and in Exp 15B normal saline (2 mL), respectively. Blood samples were taken at -15, 0, 30, 60, 90, 120, 150 and 180 minutes. After oestrogen treatment, late follicular phase serum oestradiol levels were attained on day 15 of periods A and B. Ghrelin administration did not affect serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), whereas it increased significantly those of growth hormone (GH) and PRL. In Exp 15A, serum PRL increment in response to ghrelin (area under the curve, net increment) was significantly greater than in Exp 1A (P<.05). This study demonstrates for the first time that in oestrogen-deprived postmenopausal women, ghrelin administration affects neither FSH nor LH levels but stimulates PRL secretion, that is amplified by exogenous oestrogen administration. © 2017 John Wiley & Sons Ltd.

Turbulent Aeroheating Testing of Mars Science Laboratory Entry Vehicle in Perfect-Gas Nitrogen

NASA Technical Reports Server (NTRS)

Hollis, Brian R.; Collier, Arnold S.

2007-01-01

An experimental investigation of turbulent aeroheating on the Mars Science Laboratory entry vehicle heat shield has been conducted in the Arnold Engineering Development Center Hypervelocity Wind Tunnel No. 9. Testing was performed on a 6-in. (0.1524 m) diameter MSL model in pure N2 gas in the tunnel s Mach 8 and Mach 10 nozzles at free stream Reynolds numbers of 4.1x10(exp 6)/ft to 49x10(exp 6)/ft (1.3x10(exp 7)/m to 16x10(exp 7)/m) and 1.2x10(exp 6)/ft to 19x10(exp 6)/ft (0.39x10(exp 7)/m to 62x10(exp 7)/m), respectively. These conditions were sufficient to span the regime of boundary-layer flow from completely laminar to fully-developed turbulent flow over the entire forebody. A supporting aeroheating test was also conducted in the Langley Research Center 20-Inch Mach 6 Air Tunnel at free stream Reynolds number of 1x10(exp 6)/ft to 7x10(exp 6)/ft (0.36x10(exp 7)/m to 2.2x10(exp 7)/m) in order to help corroborate the Tunnel 9 results. A complementary computational fluid dynamics study was conducted in parallel to the wind tunnel testing. Laminar and turbulent predictions were generated for all wind tunnel test conditions and comparisons were performed with the data for the purpose of helping to define uncertainty margins on predictions for aeroheating environments during entry into the Martian atmosphere. Data from both wind tunnel tests and comparisons with the predictions are presented herein. It was concluded from these comparisons that for perfect-gas conditions, the computational tools could predict fully-laminar or fully-turbulent heating conditions to within 10% of the experimental data

Turbulent Aeroheating Testing of Mars Science Laboratory Entry Vehicle

NASA Technical Reports Server (NTRS)

Hollis, Brian R.; Collier, Arnold S.

2008-01-01

An experimental investigation of turbulent aeroheating on the Mars Science Laboratory entry vehicle heat shield has been conducted in the Arnold Engineering Development Center Hypervelocity Wind Tunnel No. 9. Testing was performed on a 6-in. (0.1524 m) diameter MSL model in pure N2 gas in the tunnel's Mach 8 and Mach 10 nozzles at free stream Reynolds numbers of 4.1 x 10(exp 6)/ft to 49 x 10(exp 6)/ft (1.3 x 10(exp 7)/m to 19 x 10(exp 6/ft) and 1.2 x 10(exp 6)/ft to 19 x 10(exp 6)/ft (0.39 x 10(exp 7)/m to 62 x 10(exp 7)/m), respectively. These conditions were sufficient to span the regime of boundary-layer flow from completely laminar to fully-developed turbulent flow over the entire forebody. A supporting aeroheating test was also conducted in the Langley Research Center 20-Inch Mach 6 Air Tunnel at free stream Reynolds number of 1 x 10(exp 6)/ft to 7 x 10(exp 6)/ft (0.36 x 10(exp 7)/m to 2.2 x 10(exp 7)/m) in order to help corroborate the Tunnel 9 results. A complementary computational fluid dynamics study was conducted in parallel to the wind tunnel testing. Laminar and turbulent predictions were generated for the wind tunnel test conditions and comparisons were performed with the data for the purpose of helping to define uncertainty margins on predictions for aeroheating environments during entry into the Martian atmosphere. Data from both wind tunnel tests and comparisons with the predictions are presented herein. It was concluded from these comparisons that for perfect-gas conditions, the computational tools could predict fully-laminar or fully-turbulent heating conditions to within 12% or better of the experimental data.

Rice and soy protein isolate in pre-starter diets for broilers.

PubMed

Ebling, P D; Kessler, A M; Villanueva, A P; Pontalti, G C; Farina, G; Ribeiro, A M L

2015-11-01

Although most industries use a specific diet for the pre-starter phase, ingredients are used in the later phases, primarily corn and soybean meal, which at this stage do not have high digestibility. Three experiments : Exp : were carried out to evaluate the substitution of corn by white or parboiled rice : WR, PR : and the inclusion of 6% soy protein isolate : SPI : in pre-starter diets (1 to 7 d). In Exp 1 (1 to 21 d), WR, PR, and SPI were added to the diets until the birds were 21-day-old, whereas in Exps 2 and 3 (1 to 33 d), only until birds were 7-day-old. Birds were fed a basal diet based on corn and soybean meal until the end of the experimental period. In Exp 1, the coefficients of total tract apparent retention : CTTAR : of the pre-starter and the starter diets, ileal and jejunal digestibility of starch in the starter diets, and broiler performance were measured. Subsequently, the same pre-starter diets Exp 1 were evaluated in pellet (Exp 2) or mash (Exp 3) form and different oil percentage (Exp 2) or not (Exp 3). We hypothesized that the ingredients particle size (Exp 2) and fat content (Exp 3) could influence feed intake. There was no interaction among the evaluated factors (P > 0.05). Rice (WR or PR) promoted better results than corn in terms of CTTAR of dietary components, jejunal and ileal starch digestibility, and broiler performance (P < 0.01). In Exp 1, diets with 6% SPI presented better CTTAR, except Nitrogen ( N: ), but lower feed intake, which negatively affected broiler performance (P < 0.01). In Exps 1, 2, and 3, the inclusion of SPI did not improve broiler performance (P > 0.05), so that SPI may not be the best choice for pre-starter diets. The study also suggests that better growth performance and nutrient digestibility can be obtained in broiler chickens if corn is replaced by rice in pre-starter diets. © 2015 Poultry Science Association Inc.

Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus.

PubMed

Busse, Hans-Jürgen

2016-01-01

In this paper, the taxonomy of the genus Arthrobacter is discussed, from its first description in 1947 to the present state. Emphasis is given to intrageneric phylogeny and chemotaxonomic characteristics, concentrating on quinone systems, peptidoglycan compositions and polar lipid profiles. Internal groups within the genus Arthrobacter indicated from homogeneous chemotaxonomic traits and corresponding to phylogenetic grouping and/or high 16S rRNA gene sequence similarities are highlighted. Furthermore, polar lipid profiles and quinone systems of selected species are shown, filling some gaps concerning these chemotaxonomic traits. Based on phylogenetic groupings, 16S rRNA gene sequence similarities and homogeneity in peptidoglycan types, quinone systems and polar lipid profiles, a description of the genus Arthrobacter sensu lato and an emended description of Arthrobacter roseus are provided. Furthermore, reclassifications of selected species of the genus Arthrobacter into novel genera are proposed, namely Glutamicibacter gen. nov. (nine species), Paeniglutamicibacter gen. nov. (six species), Pseudoglutamicibacter gen. nov. (two species), Paenarthrobacter gen. nov. (six species) and Pseudarthrobacter gen. nov. (ten species).

Free-Space Green’s Functions of the Reduced Wave Equation.

DTIC Science & Technology

1982-09-01

1/2Z) exp(ino)!j J GF( ’n’axRo)Jn( r)exp(iaz)d~da- - - o (4.1) __8 * where the inverse transform is (F2,nr,)o) J (,R)J(;r exp(/in)-iz)rdrddz (4.2) -F...exp(im.) E ?m(cos 8) GF(,nmR (JR)E/2d• M-w n=-= 51 0 s n) -). where the inverse transform is G F(En,m,R) (2n+l)(n-m)!1/2/(4r2(n+m)!). 2w 7r ((RR )exp

Thermal enhancement cartridge heater modified (TECH Mod) tritium hydride bed development, Part 1 - Design and fabrication

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klein, J.E.; Estochen, E.G.

The Savannah River Site (SRS) tritium facilities have used first generation (Gen1) LaNi{sub 4.25}Al{sub 0.75} (LANA0.75) metal hydride storage beds for tritium absorption, storage, and desorption. The Gen1 design utilizes hot and cold nitrogen supplies to thermally cycle these beds. Second and third generation (Gen2 and Gen3) storage bed designs include heat conducting foam and divider plates to spatially fix the hydride within the bed. For thermal cycling, the Gen2 and Gen3 beds utilize internal electric heaters and glovebox atmosphere flow over the bed inside the bed external jacket for cooling. The currently installed Gen1 beds require replacement due tomore » tritium aging effects on the LANA0.75 material, and cannot be replaced with Gen2 or Gen3 beds due to different designs of these beds. At the end of service life, Gen1 bed desorption efficiencies are limited by the upper temperature of hot nitrogen supply. To increase end-of-life desorption efficiency, the Gen1 bed design was modified, and a Thermal Enhancement Cartridge Heater Modified (TECH Mod) bed was developed. Internal electric cartridge heaters in the new design to improve end-of-life desorption, and also permit in-bed tritium accountability (IBA) calibration measurements to be made without the use of process tritium. Additional enhancements implemented into the TECH Mod design are also discussed. (authors)« less

THERMAL ENHANCEMENT CARTRIDGE HEATER MODIFIED TECH MOD TRITIUM HYDRIDE BED DEVELOPMENT PART I DESIGN AND FABRICATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klein, J.; Estochen, E.

The Savannah River Site (SRS) tritium facilities have used 1{sup st} generation (Gen1) LaNi{sub 4.25}Al{sub 0.75} (LANA0.75) metal hydride storage beds for tritium absorption, storage, and desorption. The Gen1 design utilizes hot and cold nitrogen supplies to thermally cycle these beds. Second and 3{sup rd} generation (Gen2 and Gen3) storage bed designs include heat conducting foam and divider plates to spatially fix the hydride within the bed. For thermal cycling, the Gen2 and Gen 3 beds utilize internal electric heaters and glovebox atmosphere flow over the bed inside the bed external jacket for cooling. The currently installed Gen1 beds requiremore » replacement due to tritium aging effects on the LANA0.75 material, and cannot be replaced with Gen2 or Gen3 beds due to different designs of these beds. At the end of service life, Gen1 bed desorption efficiencies are limited by the upper temperature of hot nitrogen supply. To increase end-of-life desorption efficiency, the Gen1 bed design was modified, and a Thermal Enhancement Cartridge Heater Modified (TECH Mod) bed was developed. Internal electric cartridge heaters in the new design to improve end-of-life desorption, and also permit in-bed tritium accountability (IBA) calibration measurements to be made without the use of process tritium. Additional enhancements implemented into the TECH Mod design are also discussed.« less

A striking new genus and species of cave-dwelling frog (Amphibia: Anura: Microhylidae: Asterophryinae) from Thailand

PubMed Central

Suwannapoom, Chatmongkon; Tunprasert, Jitthep; Ruangsuwan, Thiti; Pawangkhanant, Parinya; Korost, Dmitriy V.

2018-01-01

We report on a discovery of Siamophryne troglodytes Gen. et sp. nov., a new troglophilous genus and species of microhylid frog from a limestone cave in the tropical forests of western Thailand. To assess its phylogenetic relationships we studied the 12S rRNA–16S rRNA mtDNA fragment with final alignment comprising up to 2,591 bp for 56 microhylid species. Morphological characterization of the new genus is based on examination of external morphology and analysis of osteological characteristics using microCT-scanning. Phylogenetic analyses place the new genus into the mainly Australasian subfamily Asterophryinae as a sister taxon to the genus Gastrophrynoides, the only member of the subfamily known from Sundaland. The new genus markedly differs from all other Asterophryinae members by a number of diagnostic morphological characters and demonstrates significant mtDNA sequence divergence. We provide a preliminary description of a tadpole of the new genus. Thus, it represents the only asterophryine taxon with documented free-living larval stage and troglophilous life style. Our work demonstrates that S. troglodytes Gen. et sp. nov. represents an old lineage of the initial radiation of Asterophryinae which took place in the mainland Southeast Asia. Our results strongly support the “out of Indo-Eurasia” biogeographic scenario for this group of frogs. To date, the new frog is only known from a single limestone cave system in Sai Yok District of Kanchanaburi Province of Thailand; its habitat is affected by illegal bat guano mining and other human activities. As such, S. troglodytes Gen. et sp. nov. is likely to be at high risk of habitat loss. Considering high ecological specialization and a small known range of the new taxon, we propose a IUCN Red List status of endangered for it. PMID:29497587

A striking new genus and species of cave-dwelling frog (Amphibia: Anura: Microhylidae: Asterophryinae) from Thailand.

PubMed

Suwannapoom, Chatmongkon; Sumontha, Montri; Tunprasert, Jitthep; Ruangsuwan, Thiti; Pawangkhanant, Parinya; Korost, Dmitriy V; Poyarkov, Nikolay A

2018-01-01

We report on a discovery of Siamophryne troglodytes Gen. et sp. nov., a new troglophilous genus and species of microhylid frog from a limestone cave in the tropical forests of western Thailand. To assess its phylogenetic relationships we studied the 12S rRNA-16S rRNA mtDNA fragment with final alignment comprising up to 2,591 bp for 56 microhylid species. Morphological characterization of the new genus is based on examination of external morphology and analysis of osteological characteristics using microCT-scanning. Phylogenetic analyses place the new genus into the mainly Australasian subfamily Asterophryinae as a sister taxon to the genus Gastrophrynoides , the only member of the subfamily known from Sundaland. The new genus markedly differs from all other Asterophryinae members by a number of diagnostic morphological characters and demonstrates significant mtDNA sequence divergence. We provide a preliminary description of a tadpole of the new genus. Thus, it represents the only asterophryine taxon with documented free-living larval stage and troglophilous life style. Our work demonstrates that S. troglodytes Gen. et sp. nov. represents an old lineage of the initial radiation of Asterophryinae which took place in the mainland Southeast Asia. Our results strongly support the "out of Indo-Eurasia" biogeographic scenario for this group of frogs. To date, the new frog is only known from a single limestone cave system in Sai Yok District of Kanchanaburi Province of Thailand; its habitat is affected by illegal bat guano mining and other human activities. As such, S. troglodytes Gen. et sp. nov. is likely to be at high risk of habitat loss. Considering high ecological specialization and a small known range of the new taxon, we propose a IUCN Red List status of endangered for it.

Advanced generation anti-prostate specific membrane antigen designer T cells for prostate cancer immunotherapy.

PubMed

Ma, Qiangzhong; Gomes, Erica M; Lo, Agnes Shuk-Yee; Junghans, Richard P

2014-02-01

Adoptive immunotherapy by infusion of designer T cells (dTc) engineered with chimeric antigen receptors (CARs) for tumoricidal activity represents a potentially highly specific modality for the treatment of cancer. In this study, 2nd generation (gen) anti-prostate specific membrane antigen (PSMA) dTc were developed for improving the efficacy of previously developed 1st gen dTc for prostate cancer immunotherapy. The 1st gen dTc are modified with chimeric immunoglobulin-T cell receptor (IgTCR) while the 2nd gen dTc are engineered with an immunoglobulin-CD28-T cell receptor (IgCD28TCR), which incorporates a CD28 costimulatory signal for optimal T cell activation. A 2nd gen anti-PSMA IgCD28TCR CAR was constructed by inserting the CD28 signal domain into the 1st gen CAR. 1st and 2nd gen anti-PSMA dTc were created by transducing human T cells with anti-PSMA CARs and their antitumor efficacy was compared for specific activation on PSMA-expressing tumor contact, cytotoxicity against PSMA-expressing tumor cells in vitro, and suppression of tumor growth in an animal model. The 2nd gen dTc can be optimally activated to secrete larger amounts of cytokines such as IL2 and IFNγ than 1st gen and to proliferate more vigorously on PSMA-expressing tumor contact. More importantly, the 2nd gen dTc preserve the PSMA-specific cytotoxicity in vitro and suppress tumor growth in animal models with significant higher potency. Our results demonstrate that 2nd gen anti-PSMA designer T cells exhibit superior antitumor functions versus 1st gen, providing a rationale for advancing this improved agent toward clinical application in prostate cancer immunotherapy. © 2013 Wiley Periodicals, Inc.

Genistein promotes DNA demethylation of the steroidogenic factor 1 (SF-1) promoter in endometrial stromal cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matsukura, Hiroshi, E-mail: hmatsukura.epi@mri.tmd.ac.jp; Aisaki, Ken-ichi; Igarashi, Katsuhide

2011-08-26

Highlights: {yields} Genistein (GEN) is a phytoestrogen found in soy products. {yields} GEN demethylated/unsilenced the steroidogenic factor 1 gene in endometrial tissue. {yields} GEN thus altered mRNA expression in uteri of ovariectomized (OVX) mice. {yields} A high-resolution melting assay was used to screen for epigenetic change. {yields} We isolated an endometrial cell clone that was epigenetically modulated by GEN. -- Abstract: It has recently been demonstrated that genistein (GEN), a phytoestrogen in soy products, is an epigenetic modulator in various types of cells; but its effect on endometrium has not yet been determined. We investigated the effects of GEN onmore » mouse uterine cells, in vivo and in vitro. Oral administration of GEN for 1 week induced mild proliferation of the endometrium in ovariectomized (OVX) mice, which was accompanied by the induction of steroidogenic factor 1 (SF-1) gene expression. GEN administration induced demethylation of multiple CpG sites in the SF-1 promoter; these sites are extensively methylated and thus silenced in normal endometrium. The GEN-mediated promoter demethylation occurred predominantly on the luminal side, as opposed to myometrium side, indicating that the epigenetic change was mainly shown in regenerated cells. Primary cultures of endometrial stromal cell colonies were screened for GEN-mediated alterations of DNA methylation by a high-resolution melting (HRM) method. One out of 20 colony-forming cell clones showed GEN-induced demethylation of SF-1. This clone exhibited a high proliferation capacity with continuous colony formation activity through multiple serial clonings. We propose that only a portion of endometrial cells are capable of receiving epigenetic modulation by GEN.« less

Genistein supplementation increases bone turnover but does not prevent alcohol-induced bone loss in male mice.

PubMed

Yang, Carrie S; Mercer, Kelly E; Alund, Alexander W; Suva, Larry J; Badger, Thomas M; Ronis, Martin J J

2014-10-01

Chronic alcohol consumption results in bone loss through increased bone resorption and decreased bone formation. These effects can be reversed by estradiol (E2) supplementation. Soy diets are suggested to have protective effects on bone loss in men and women, as a result of the presence of soy protein-associated phytoestrogens such as genistein (GEN). In this study, male mice were pair-fed (PF), a control diet, an ethanol (EtOH) diet, or EtOH diet supplemented with 250 mg/kg of GEN for 8 weeks to test if GEN protects against bone loss associated with chronic drinking. Interestingly, alcohol consumption reduced cortical area and thickness and trabecular bone volume in both EtOH and EtOH/GEN groups when compared to the corresponding PF and PF/GEN controls, P < 0.05. However, in the trabecular bone compartment, we observed a significant increase in overall trabecular bone density in the PF/GEN group compared to the PF controls. Bone loss in the EtOH-treated mice was associated with the inhibition of osteoblastogenesis as indicated by decreased alkaline phosphatase staining in ex vivo bone marrow cultures, P < 0.05. GEN supplementation improved osteoblastogenesis in the EtOH/GEN cultures compared to the EtOH group, P < 0.05. Vertebral expression of bone-formation markers, osteocalcin, and runt-related transcription factor 2 (Runx2) was also significantly up-regulated in the PF/GEN and EtOH/GEN groups compared to the PF and EtOH-treated groups. GEN supplementation also increased the expression of receptor activator of nuclear factor κ-B ligand (RANKL) in the PF/GEN, an increase that persisted in the EtOH/GEN-treated animals (P < 0.05), and increased basal hydrogen peroxide production and RANKL mRNA expression in primary bone marrow cultures in vitro, P < 0.05. These findings suggest that GEN supplementation increases the overall bone remodeling and, in the context of chronic alcohol consumption, does not protect against the oxidative stress-associated EtOH-mediated bone resorption. © 2014 by the Society for Experimental Biology and Medicine.

Stratospheric OClO and NO2 measured by groundbased UV/Vis-spectroscopy in Greenland in January and February 1990 and 1991

NASA Technical Reports Server (NTRS)

Roth, A.; Perner, D.

1994-01-01

Groundbased UV/Vis-spectroscopy of zenith scattered sunlight was performed at Sondre Stromfjord (Greenland) during Jan/Feb 1990 and Jan/Feb 1991. Considerable amounts of OClO were observed during both campaigns. Maximum OClO vertical column densities at 92 deg solar zenith angle (SZA) were 7.4 x 10(exp 13) molec/sq cm in 1990 and 5.7 x 10(exp 13) molec/sq cm in 1991 (chemical enhancement is included in the calculation of the air mass factor (AMF)). A threshold seems to exist for OClO detection: OClO was detected on every day when the potential vorticity at the 475 K level of potential temperature was higher than 35 x 10(exp -6)Km(exp 2)kg(exp -1)s(exp -1). NO2 vertical columns lower than 1 x 10(exp 15) molec/sq cm were frequently observed in both winters.

Rosat observations of FK comae berenices

NASA Technical Reports Server (NTRS)

Welty, Alan D.; Ramsey, Lawrence W.

1994-01-01

We obtained ROSAT PSPC observations of FK Com over a period of 24.4 h, or 0.42 rotation. During the observations the x-ray flux increased by a factor of at least 5 before declining toward its previous level. A single temperature Raymond-Smith model is adequate to model the low signal-to-noise ratio spectrum from each observation interval. Initially the spectrum was that of a 8.5 x 10(exp 6) K plasma, with L9sub x)=0.66 x 10(exp 31) erg s(exp -1). When the x-ray flux was greatest, the model plasma temperature rose to 2.5 x 10(exp 7) K, and L(sub x)=3.46 x 10(exp 31) ergs(exp -1). During the post-maximum decline in luminosity the plasma temperature was approximately 12 x 10(exp 6) K. We conclude that the increase of x-ray flux recorded by ROSAT was due to an x-ray flare with a 1.5 h decline time scale.

Small bilaterian fossils from 40 to 55 million years before the cambrian.

PubMed

Chen, Jun-Yuan; Bottjer, David J; Oliveri, Paola; Dornbos, Stephen Q; Gao, Feng; Ruffins, Seth; Chi, Huimei; Li, Chia-Wei; Davidson, Eric H

2004-07-09

Ten phosphatized specimens of a small (<180 micrometers) animal displaying clear bilaterian features have been recovered from the Doushantuo Formation, China, dating from 40 to 55 million years before the Cambrian. Seen in sections, this animal (Vernanimalcula guizhouena gen. et sp. nov.) had paired coeloms extending the length of the gut; paired external pits that could be sense organs; bilateral, anterior-posterior organization; a ventrally directed anterior mouth with thick walled pharynx; and a triploblastic structure. The structural complexity is that of an adult rather than a larval form. These fossils provide the first evidence confirming the phylogenetic inference that Bilateria arose well before the Cambrian.

The Integrated Airport: Building a Successful NextGen Testbed

DOE Office of Scientific and Technical Information (OSTI.GOV)

Frederick-Recascino, Christina; Sweigard, Doug; Lester, Wade

2009-02-18

This presentation will describe a unique public-private partnership - the Integrated Airport - that was created to engage in research and testing related to NextGen Technology deployment.  NextGen refers to the program that will be initiated to modernize the US National Airspace.  As with any major, multi-decade initiative, such as NextGen, integration of work efforts by multiple partners in the modernization is critical for success.  This talk will focus on the development of the consortium, how the consortium plans for NextGen initiatives, the series of technology demonstrations we have produced and plans for the future of NextGen testing and implementation. 

Records of Tortricidae from the Afrotropical region, with descriptions of new taxa (Lepidoptera)

USDA-ARS?s Scientific Manuscript database

Four new genera (Bichrompotamia Razowski & Brown, gen. n., Nkandla Razowski & Brown, gen. n., Vacanara Razowski & Brown, gen. n., Birutia Razowski & Brown, gen. n.) and six new species (Bichrompotamia rhodesiae Razowski & Brown, sp. n., Vacanara caravanica Razowski & Brown, sp. n., Birutia meridafri...

Analysis of Harmonic Distortion in an Integrated Power System for Naval Applications

DTIC Science & Technology

2005-06-01

tdop/xd; tdpp =xdpp*tdopp/xdp; ial =( 1/xd+( 1/Xdp- l/Xd). *exp(-t./tdp)+( l/xdpp- 1/Xdp). *ex p(-t./ tdpp )). *cos(377*t).. -1 ./xdpp*exp(-t./ta); hold...title(’Generator Fault Current’) ylabel(’Phase Current (pu)’) xdpp=. 15; tdp=xdp*tdop/xd; tdpp =xdpp*tdopp/xdp; ial =( 1/xd+( 1/xdp- 1/xd). *exp(-t./tdp...1/Xdpp- 1/xdp). *exp(-t./ tdpp )). *cos(377*t)... -1 ./xdpp*exp(-t./ta); plot(t,-ia 1) h=legend(󈧝% subtransient reactance’,’ 15% subtransient

J3Gen: A PRNG for Low-Cost Passive RFID

PubMed Central

Melià-Seguí, Joan; Garcia-Alfaro, Joaquin; Herrera-Joancomartí, Jordi

2013-01-01

Pseudorandom number generation (PRNG) is the main security tool in low-cost passive radio-frequency identification (RFID) technologies, such as EPC Gen2. We present a lightweight PRNG design for low-cost passive RFID tags, named J3Gen. J3Gen is based on a linear feedback shift register (LFSR) configured with multiple feedback polynomials. The polynomials are alternated during the generation of sequences via a physical source of randomness. J3Gen successfully handles the inherent linearity of LFSR based PRNGs and satisfies the statistical requirements imposed by the EPC Gen2 standard. A hardware implementation of J3Gen is presented and evaluated with regard to different design parameters, defining the key-equivalence security and nonlinearity of the design. The results of a SPICE simulation confirm the power-consumption suitability of the proposal. PMID:23519344

Fuel Cells Advanced Drivetrain

DTIC Science & Technology

2010-08-09

T800 Silverado Military specific system upscale (4WD) HydroGen3 – CH2 HydroGen3 – LH2 4010 – Early Adopter Engineering Model (GEN Nadeau) Gen4...Timeline Maintenance Infrastructure Fort Belvoir, VA 2003-ongoing Full FCV and Volt Maintenance 350,700bar LH2 (Decommissioned) Volt Charging(pending

75 FR 52790 - Small Business Size Standards: Waiver of the Nonmanufacturer Rule

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-27

... for GEN II and GEN III Image Intensifier Tubes. SUMMARY: The U.S. Small Business Administration (SBA... Image Intensifier Tubes, Product Service Code (PSC) 5855, Night Vision Equipment, Emitted and Reflected... business GEN II and GEN III image intensifier tube manufacturers. If granted, the waiver would allow...

40 CFR 80.45 - Complex emissions model.

Code of Federal Regulations, 2010 CFR

2010-07-01

... this section for the appropriate phase and season Nd = exp d1(t)/exp d1(b) Hd = exp d2(t)/exp d2(b) w1.... d2(t) = Higher emitter 1,3-butadiene equation as defined in paragraph (e)(7)(ii) of this section... the base fuel's properties. d2(b) = Higher emitter 1,3-butadiene equation as defined in paragraph (e...

Occurrence of white striping and wooden breast in broilers fed grower and finisher diets with increasing lysine levels.

PubMed

Cruz, R F A; Vieira, S L; Kindlein, L; Kipper, M; Cemin, H S; Rauber, S M

2017-02-01

Two experiments were conducted to evaluate the prevalence and severity of white striping (WS) and wooden breast (WB) in breast fillets from broilers fed diets with increasing digestible Lysine (dLys) from 12 to 28 d (Exp. 1) and from 28 to 42 d (Exp. 2). Trials were sequentially conducted using one-d-old male, slow-feathering Cobb 500 × Cobb broilers, both with 6 treatments and 8 replicates. Increasing dLys levels were equally spaced from 0.77 to 1.17% in Exp. 1 and from 0.68 to 1.07% in Exp. 2. The lowest dLys diet was not supplemented with L-Lysine (L-Lys) in either one of the studies and all other essential amino acid (AA) met or exceeded current commercial recommendations such that their dietary concentrations did not limit broiler growth. Four birds per pen were randomly selected from each replication and processed at 35 and 42 d in Exp. 1 and Exp. 2, respectively. Deboned breast fillets (Pectoralis major) were submitted to a 3 subject panel evaluation to detect the presence of WS and WB, as well as to provide scores of WS (0-normal, 1-moderate, 2-severe) and WB (0-normal, 1-moderate light, 2-moderate, 3-severe). Increasing the level of dLys had a positive effect on BW, carcass, and breast weight, as well as breast yield. White striping and WB prevalences were 32.3 and 85.9% in Exp. 1 and 87.1 and 89.2% in Exp. 2. Birds fed diets not supplemented with L-Lys had the lowest average WS and WB scores (0.22 and 0.78 in Exp. 1 and 0.61 and 0.68 in Exp. 2). White striping and WB presented linear responses to performance variables in Exp. 1, whereas quadratic responses were observed for all variables in Exp. 2. In conclusion, increasing the level of dLys improved growth performance and carcass traits as well as induced the occurrence and severity of WS and WB lesions. © 2016 Poultry Science Association Inc.

Effect of feeding an encapsulated source of butyric acid (ButiPEARL) on the performance of male Cobb broilers reared to 42 d of age.

PubMed

Levy, April Waguespack; Kessler, James W; Fuller, Lorraine; Williams, Susan; Mathis, Greg F; Lumpkins, Brett; Valdez, Fernando

2015-08-01

The objective of this research was to determine the optimal level of an encapsulated butyric acid (ButiPEARL) based on the performance of male Cobb broilers reared to 42 d of age and to investigate its effects on intestinal morphology. Experiment 1 ( EXP 1: ) consisted of 4 treatments with 12 replicate pens that contained 45 broilers, and Experiment 2 ( EXP 2: ) consisted of 6 treatments with 8 replicate pens that contained 50 broilers. Birds were weighed by pen on d 0, 21, 35, and 42. In EXP 1, the treatments were as follows: 1) control ( C: ); 2) C + 100 g ButiPEARL/ton; 3) C + 200 g ButiPEARL/ton; and 4) C + 300 g ButiPEARL/ton. In EXP 2, the treatments were identical to EXP 1, with 2 additional treatments: 5) C + 400 g ButiPEARL/ton and 6) C + 500 g ButiPEARL/ton. In EXP 1, two 42-d-old broilers per pen were randomly selected for duodenal and jejunal tissue collection. Only the samples from the broilers fed the C or 300 g ButiPEARL treatments were analyzed for histology in EXP 1. For EXP 2, on d 21 and 35, two broilers per pen were randomly selected for duodenal, jejunal, and ileal tissue collection. For EXP 1 and 2, BW gain increased linearly with increasing butyric acid levels (P < 0.027 and P < 0.001, respectively). For EXP 1 and 2, feed conversion linearly improved with increasing butyric acid from 0 to 42 d (P < 0.001 and P < 0.001, respectively). In EXP 1, there were no differences in any intestinal morphology at 42 d between broilers fed the C or 300 g ButiPEARL treatments. In EXP 2, there were no differences in villus height at 21 or 35 d of age with any level of butyric acid. Based on the results of this research related to BW gain and feed conversion, the recommended optimum dosage level for ButiPEARL in broilers reared to 42 d of age is up to 500 g/ton. © 2015 Poultry Science Association Inc.

Highly active antiretroviral therapy during gestation: effects on a rat model of pregnancy.

PubMed

Carvalho, L P F; Simões, R S; Araujo Júnior, E; Oliveira Filho, R M; Kulay Júnior, L; Nakamura, M U

2014-04-01

To assess the adverse effects of the chronic use of zidovudine/lopinavir/ritonavir in a rat pregnancy model.Type of article: Original paper. A prospective experimental study. Department of Obstetrics, São Paulo Federal University (UNIFESP). 40 pregnant EPM-1 albino rats were randomly allocated into four groups of 10 animals each: control (Ctrl) group (untreated) and three experimental groups (Exp1, Exp2 and Exp3), which received zidovudine/lopinavir/ritonavir in the corresponding doses of 10/13.3/3.3; 30/39.9/9.9 and 90/119.7/29.7 mg/Kg/day from the first up to the 20th day of pregnancy, respectively. The rats were treated by gavage daily. Body weights were recorded on days 0, 7, 14 and 20. At term, the rats were sacrificed and the implantation sites, number of live and dead fetuses and placentas, resorptions and fetal and placental weights were recorded. The fetuses were evaluated for external abnormalities under a stereomicroscope. The chi-square test was used to compare death rates between groups. Weight gain during pregnancy no showed significant differences between groups. Average weight gains between the 7th and 20th day were 45.70 ± 5.27 g for Ctrl; 48.49 ± 3.64 g for Exp1; 45.39 ± 6.22 g for Exp2 and 44.19 ± 6.78 g for Exp3. However, the percentage weight gain in the 7th was lower in groups Exp2 and Exp3 and in the 14th in the Group Exp2. All other parameters assessed did not differ significantly between groups. Exp2 and Exp3 in relation of the others. The chronic exposure of pregnant rats to high doses of zidovudine/lopinavir/ritonavir in association resulted in a significant reduction in maternal body weight gain but was not associated with significant adverse fetal parameters.

A Virtual Reality Framework to Optimize Design, Operation and Refueling of GEN-IV Reactors.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rizwan-uddin; Nick Karancevic; Stefano Markidis

2008-04-23

many GEN-IV candidate designs are currently under investigation. Technical issues related to material, safety and economics are being addressed at research laboratories, industry and in academia. After safety, economic feasibility is likely to be the most important crterion in the success of GEN-IV design(s). Lessons learned from the designers and operators of GEN-II (and GEN-III) reactors must play a vital role in achieving both safety and economic feasibility goals.

Self-Broadening and Self-Shift Coefficients in the Fundamental Band of 12C 16O

NASA Technical Reports Server (NTRS)

Devi, Malathy V.; Benner, D. Chris; Smith, Mary Ann H.; Rinsland, Curtis P.

1998-01-01

High quality and precise measurements of self-broadened and self-shift coefficients in the fundamental band of C-12O-16 were made using spectra recorded at room temperature with the high-resolution (0.0027 cm(exp -1)) McMath-Pierce Fourier transform spectrometer located at the National Solar Observatory on Kitt Peak, Arizona. The spectral region under investigation (2008-2247 cm(exp -1)) contains the P(31) to R(31) transitions. The data were obtained using a high-purity natural isotopic sample ofcarbon monoxide and two absorption cells with pathlengths of 4.08 and 9.98 cm, respectively. Various pressures of CO were used, ranging between 0.25 and 201.2 Torr. The results were obtained by analyzing five spectra simultaneously, using a multispectrum nonlinear least-squares fitting technique. The self-broadened coefficients ranged from 0.0426(2) cm(exp -1) atm(exp -1) at 296 K to 0.0924(2) cm(exp -1) atm(exp -1) at 296 K, while the pressure-induced shift coefficients varied between -0.0042(3) cm(exp -1) atm(exp -1) at 296 K and +0.0005(l) cm(exp -1) atm(exp -1) at 296 K. The value in parentheses is the estimated uncertainty in units of the last digit. The self-broadened coefficients of lines with same values of m in the P and R branches agree close to within experimental uncertainties while the self-shift coefficients showed considerable variation within and between the two branches. The mean value of the ratios of P branch to R branch self-broadened coefficients was found to be 1.01 with a standard deviation of + or - 0.01. Comparisons of the results with other published data were made.

The High Energy Spectrum of NGC 4151

NASA Technical Reports Server (NTRS)

Beckmann, V.; Gehrels, N.; Shrader, C.; Soldi, S.; Lubinski, P.; Zdziarski, A. A.; Petrucci, P.-O.; Malzac, J.

2005-01-01

We present first INTEGRAL observations of the type 1.5 Seyfert galaxy NGC 4151. Combining several INTEGRAL observations performed during 2003, totaling approximately 400 ksec of exposure time, allow us to study the spectrum in the 3 - 300 keV range. The measurements presented here reveal an overall spectrum from X-rays up to the soft gamma-rays that can be described by an absorbed (N(sub H) approximately equal to 5 x 10(exp 22) per square centimeter) and non-variable thermal component, plus a Fe Kalpha line, and an exponential cutoff occurs at 110 keV, consistent with earlier claims. The Galactic hydrogen column density in the line of sight is N(sub H), Gal approximately equal to 2.1 x 10 (exp 20) per square centimeter. The time resolved analysis shows little variation of the spectral parameters. The comparison with CGRO/OSSE data shows that the same spectral model can be applied over a time span of 15 years, while the flux varied by a factor of 2. Applying a Compton reflection component improves the model fit to the INTEGRAL data. Nonetheless the data available to date cannot significantly confirm or exclude the existence of reflection, nor is a high iron overabundance in the absorber, as had been previously suggested, clearly detectable.

Influence of gene flow on divergence dating - implications for the speciation history of Takydromus grass lizards.

PubMed

Tseng, Shu-Ping; Li, Shou-Hsien; Hsieh, Chia-Hung; Wang, Hurng-Yi; Lin, Si-Min

2014-10-01

Dating the time of divergence and understanding speciation processes are central to the study of the evolutionary history of organisms but are notoriously difficult. The difficulty is largely rooted in variations in the ancestral population size or in the genealogy variation across loci. To depict the speciation processes and divergence histories of three monophyletic Takydromus species endemic to Taiwan, we sequenced 20 nuclear loci and combined with one mitochondrial locus published in GenBank. They were analysed by a multispecies coalescent approach within a Bayesian framework. Divergence dating based on the gene tree approach showed high variation among loci, and the divergence was estimated at an earlier date than when derived by the species-tree approach. To test whether variations in the ancestral population size accounted for the majority of this variation, we conducted computer inferences using isolation-with-migration (IM) and approximate Bayesian computation (ABC) frameworks. The results revealed that gene flow during the early stage of speciation was strongly favoured over the isolation model, and the initiation of the speciation process was far earlier than the dates estimated by gene- and species-based divergence dating. Due to their limited dispersal ability, it is suggested that geographical isolation may have played a major role in the divergence of these Takydromus species. Nevertheless, this study reveals a more complex situation and demonstrates that gene flow during the speciation process cannot be overlooked and may have a great impact on divergence dating. By using multilocus data and incorporating Bayesian coalescence approaches, we provide a more biologically realistic framework for delineating the divergence history of Takydromus. © 2014 John Wiley & Sons Ltd.

The Integrated Airport: Building a Successful NextGen Testbed

ScienceCinema

Frederick-Recascino, Christina; Sweigard, Doug; Lester, Wade

2018-01-24

This presentation will describe a unique public-private partnership - the Integrated Airport - that was created to engage in research and testing related to NextGen Technology deployment.  NextGen refers to the program that will be initiated to modernize the US National Airspace.  As with any major, multi-decade initiative, such as NextGen, integration of work efforts by multiple partners in the modernization is critical for success.  This talk will focus on the development of the consortium, how the consortium plans for NextGen initiatives, the series of technology demonstrations we have produced and plans for the future of NextGen testing and implementation. 

The response regulator expM is essential for the virulence of Erwinia carotovora subsp. carotovora and acts negatively on the sigma factor RpoS (sigma s).

PubMed

Andersson, R A; Palva, E T; Pirhonen, M

1999-07-01

The main virulence factors of Erwinia carotovora subsp. carotovora, the secreted, extracellular cell-wall-degrading enzymes, are controlled by several regulatory mechanisms. We have isolated transposon mutants with reduced virulence on tobacco. One of these mutants, with a mutation in a gene designated expM, was characterized in this study. This mutant produces slightly reduced amounts of extracellular enzymes in vitro and the secretion of the enzymes is also affected. The expM wild-type allele was cloned together with an upstream gene, designated expL, that has an unknown function. The expM gene was sequenced and found to encode a protein with similarity to the RssB/SprE protein of Escherichia coli and the MviA protein of Salmonella typhimurium. These proteins belong to a new type of two-component response regulators that negatively regulate the stability of the Sigma factor RpoS (sigma s) at the protein level. The results of this study suggest that ExpM has a similar function in E. carotovora subsp. carotovora. We also provide evidence that the overproduction of RpoS in the expM mutant is an important factor for the reduced virulence phenotype and that it partly causes the observed phenotype seen in vitro. However, an expM/rpoS double mutant is still affected in secretion of extracellular enzymes, suggesting that ExpM in addition to RpoS also acts on other targets.

GenBank

PubMed Central

Benson, Dennis A.; Karsch-Mizrachi, Ilene; Lipman, David J.; Ostell, James; Wheeler, David L.

2007-01-01

GenBank (R) is a comprehensive database that contains publicly available nucleotide sequences for more than 240 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or standalone Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the EMBL Data Library in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI Homepage (). PMID:17202161

Silurian sponges and some associated fossils from the Heceta Limestone, Prince of Wales Island, southeastern Alaska

USGS Publications Warehouse

Rigby, J.K.; Rohr, D.M.; Blodgett, R.B.; Britt, B.B.

2008-01-01

A small faunule of hypercalcified agelasiid demosponges has been recovered from outcrops of the Silurian Heceta Formation on Prince of Wales Island in southeastern Alaska. Included are abundant Girtyocoeliana epiporata (Rigby and Potter, 1986), of the Girtyocoeliidae Finks and Rigby, 2004; fragments of Alaskaspongiella laminosa n. gen. and sp., Polyplacospongia nodosa n. gen. and sp., and Monolaminospongia gigantia n. gen. and sp., of the Auriculospongiidae Termier and Termier, 1977, and Cladospongia alaskensis n. gen. and sp., Virgulaspongia uniforma n. gen. and sp., and Stipespongia laminata n. gen. and sp. of the Preperonidellidae Finks and Rigby, 2004. Also included are a few fossils of uncertain taxonomic placement, including Turbospongia biperforata n. gen. and sp., along with a small, chambered, tubular fragment and several porous tubular stems that may be additional poriferans. Some isolated octactine-based heteractinid spicules were also recovered from the etched residues. Copyright ?? 2008, The Paleontological Society.

Effect of BCAA intake during endurance exercises on fatigue substances, muscle damage substances, and energy metabolism substances

PubMed Central

Kim, Dong-Hee; Kim, Seok-Hwan; Jeong, Woo-Seok; Lee, Ha-Yan

2013-01-01

The increase rate of utilization of branched-chain amino acids (BCAA) by muscle is reduced to its plasma concentration during prolonged exercise leading to glycogen. BCAA supplementation would reduce the serum activities of intramuscular enzymes associated with muscle damage. To examine the effects of BCAA administration on fatigue substances (serotonin, ammonia and lactate), muscle damage substances (CK and LDH) and energy metabolism substances (FFA and glucose) after endurance exercise. Subjects (n = 26, college-aged males) were randomly divided into an experimental (n = 13, EXP) and a placebo (n = 13, CON) group. Subjects both EXP and CON performed a bout of cycle training (70% VO2max intensity) to exhaustion. Subject in the EXP were administrated BCAA (78ml/kg·w) prior to the bout of cycle exercise. Fatigue substances, muscle damage substances and energy metabolism substances were measured before ingesting BCAAs and placebos, 10 min before exercise, 30 min into exercise, immediately after exercise, and 30 min after exercise. Data were analyzed by two-way repeated measure ANCOVA, correlation and statistical significance was set at p < 0.05. The following results were obtained from this study; 1. In the change of fatigue substances : Serotonin in the EXP tended to decreased at the 10 min before exercise, 30 min into exercise, post exercise, and recovery 30 min. Serotonin in the CON was significantly greater than the EXP at the10 min before exercise and recovery 30. Ammonia in the EXP was increased at the 10 min before exercise, 30 min into exercise, and post exercise, but significantly decreased at the recovery 30min (p < 0.05). Ammonia in the CON was significantly lower than the EXP at the 10 min before exercise, 30 min into exercise, and post exercise (p < 0.05). Lactate in the EXP was significantly increased at the 30 min into exercise and significantly decreased at the post exercise and recovery 30 min. Lactate in the CON was significantly lower than the EXP at the post exercise (p < 0.05). 2. In the change of muscle damage substances : CK in the EXP was decreased at the 10 min before exercise and increased at the 30 min into exercise and then decreased at the post exercise and recovery 30 min. CK in the CON was greater than the EXP. LDH in the EXP was decreased at the 10 min before exercise and increased at the 30 min into exercise and then decreased at the post exercise and recovery 30 min. LDH in the CON was higher than the EXP. 3. In the change of energy metabolism substances :Glucose in the EXP tended to decrease at the 10 min before exercise, 30 min into exercise, post exercise and recovery 30 min. Glucose in the CON was significantly greater than the EXP at the recovery 30 min (p < .05). FFA in both EXP and CON was increased at the post exercise and recovery 30 min. % increase for FFA in the EXP was greater than the CON at the post exercise and recovery 30 min. 4. The relationship of the fatigue substances, muscle damage substances and energy metabolism substances after endurance exercise indicated strongly a positive relationship between LDH and ammonia and a negative relationship between LDH and FFA in the EXP. Also, there were a strong negative relationship between glucose and FFA and a positive relationship between glucose and serotonin in the EXP. There was a strong positive relationship between CK and LDH and a strong negative relationship between FFA and glucose in the CON. These results indicate that supplementary BCAA decreased serum concentrations of the intramuscular enzymes as CK and LDH following exhaustive exercise. This observation suggests that BCAA supplementation may reduce the muscle damage associated with endurance exercise. PMID:25566428

Effect of BCAA intake during endurance exercises on fatigue substances, muscle damage substances, and energy metabolism substances.

PubMed

Kim, Dong-Hee; Kim, Seok-Hwan; Jeong, Woo-Seok; Lee, Ha-Yan

2013-12-01

The increase rate of utilization of branched-chain amino acids (BCAA) by muscle is reduced to its plasma concentration during prolonged exercise leading to glycogen. BCAA supplementation would reduce the serum activities of intramuscular enzymes associated with muscle damage. To examine the effects of BCAA administration on fatigue substances (serotonin, ammonia and lactate), muscle damage substances (CK and LDH) and energy metabolism substances (FFA and glucose) after endurance exercise. Subjects (n = 26, college-aged males) were randomly divided into an experimental (n = 13, EXP) and a placebo (n = 13, CON) group. Subjects both EXP and CON performed a bout of cycle training (70% VO2max intensity) to exhaustion. Subject in the EXP were administrated BCAA (78ml/kg·w) prior to the bout of cycle exercise. Fatigue substances, muscle damage substances and energy metabolism substances were measured before ingesting BCAAs and placebos, 10 min before exercise, 30 min into exercise, immediately after exercise, and 30 min after exercise. Data were analyzed by two-way repeated measure ANCOVA, correlation and statistical significance was set at p < 0.05. The following results were obtained from this study; 1. In the change of fatigue substances : Serotonin in the EXP tended to decreased at the 10 min before exercise, 30 min into exercise, post exercise, and recovery 30 min. Serotonin in the CON was significantly greater than the EXP at the10 min before exercise and recovery 30. Ammonia in the EXP was increased at the 10 min before exercise, 30 min into exercise, and post exercise, but significantly decreased at the recovery 30min (p < 0.05). Ammonia in the CON was significantly lower than the EXP at the 10 min before exercise, 30 min into exercise, and post exercise (p < 0.05). Lactate in the EXP was significantly increased at the 30 min into exercise and significantly decreased at the post exercise and recovery 30 min. Lactate in the CON was significantly lower than the EXP at the post exercise (p < 0.05). 2. In the change of muscle damage substances : CK in the EXP was decreased at the 10 min before exercise and increased at the 30 min into exercise and then decreased at the post exercise and recovery 30 min. CK in the CON was greater than the EXP. LDH in the EXP was decreased at the 10 min before exercise and increased at the 30 min into exercise and then decreased at the post exercise and recovery 30 min. LDH in the CON was higher than the EXP. 3. In the change of energy metabolism substances :Glucose in the EXP tended to decrease at the 10 min before exercise, 30 min into exercise, post exercise and recovery 30 min. Glucose in the CON was significantly greater than the EXP at the recovery 30 min (p < .05). FFA in both EXP and CON was increased at the post exercise and recovery 30 min. % increase for FFA in the EXP was greater than the CON at the post exercise and recovery 30 min. 4. The relationship of the fatigue substances, muscle damage substances and energy metabolism substances after endurance exercise indicated strongly a positive relationship between LDH and ammonia and a negative relationship between LDH and FFA in the EXP. Also, there were a strong negative relationship between glucose and FFA and a positive relationship between glucose and serotonin in the EXP. There was a strong positive relationship between CK and LDH and a strong negative relationship between FFA and glucose in the CON. These results indicate that supplementary BCAA decreased serum concentrations of the intramuscular enzymes as CK and LDH following exhaustive exercise. This observation suggests that BCAA supplementation may reduce the muscle damage associated with endurance exercise.

A Comparison of the Single-sided (Gen II) and Double-sided (Gen I) Combat Arms Earplugs (CAE): Acoustic Properties, Human Performance, and User Acceptance

DTIC Science & Technology

2012-12-01

results of steady-state and impulse noise attenuation objective and real-ear measurements; localization and speech intelligibility human performance... Intelligibility 12 6.1 Method...12 Figure 8. Speech intelligibility testing of Gen I CAE and Gen II CAE

Quantum Well Infrared Photodetectors (QWIP)

NASA Technical Reports Server (NTRS)

Levine, B. F.

1990-01-01

There has been a lot of interest in III-V long wavelength detectors in the lambda = 8 to 12 micron spectral range as alternatives to HgCdTe. Recently high performance quantum well infrared photodetectors (QWIP) have been demonstrated. They have a responsivity of R = 1.2 A/W, and a detectivity D(exp asterisk) sub lambda = 2 times 10(exp 10) cm Hz(exp 1/2)/W at 68 K for a QWIP with a cutoff wavelength of lambda sub c = 10.7 micron and a R = 1.0 A/W, and D(exp asterisk) sub lambda = 2 times 10(exp 10) cm Hz(exp 1/2)/W at T = 77 K for lambda sub c = 8.4 micron. These detectors consist of 50 periods of molecular beam epitaxy (MBE) grown layers doped n = 1 times 10(exp 18)cm(exp -3) having GaAs quantum well widths of 40 A and barrier widths of 500 A of Al sub x Ga sub 1-x As. Due to the well-established GaAs growth and processing techniques, these detectors have the potential for large, highly uniform, low cost, high performance arrays as well as monolithic integration with GaAs electronics, high speed and radiation hardness. Latest results on the transport physics, device performance and arrays are discussed.

Quantum Well Infrared Photodetectors (QWIP)

NASA Astrophysics Data System (ADS)

Levine, B. F.

1990-07-01

There has been a lot of interest in III-V long wavelength detectors in the lambda = 8 to 12 micron spectral range as alternatives to HgCdTe. Recently high performance quantum well infrared photodetectors (QWIP) have been demonstrated. They have a responsivity of R = 1.2 A/W, and a detectivity D(exp asterisk) sub lambda = 2 times 10(exp 10) cm Hz(exp 1/2)/W at 68 K for a QWIP with a cutoff wavelength of lambda sub c = 10.7 micron and a R = 1.0 A/W, and D(exp asterisk) sub lambda = 2 times 10(exp 10) cm Hz(exp 1/2)/W at T = 77 K for lambda sub c = 8.4 micron. These detectors consist of 50 periods of molecular beam epitaxy (MBE) grown layers doped n = 1 times 10(exp 18)cm(exp -3) having GaAs quantum well widths of 40 A and barrier widths of 500 A of Al sub x Ga sub 1-x As. Due to the well-established GaAs growth and processing techniques, these detectors have the potential for large, highly uniform, low cost, high performance arrays as well as monolithic integration with GaAs electronics, high speed and radiation hardness. Latest results on the transport physics, device performance and arrays are discussed.

Fall-Back Disks in Long and Short GRBS

NASA Technical Reports Server (NTRS)

Cannizo, John K.; Troja, E.; Gehrels, N.

2011-01-01

We present numerical time-dependent calculations for fall-back disks relevant for GRBs in which the disk of material surrounding the black hole (BH) powering the GRB jet modulates the mass flow, and hence the strength of the jet. Given the initial existence of a small mass appr oximately less than 10(exp -4) M(solar) near the progenitor with a circularization radius approximately 10(exp 10) - 10(exp 11) cm, an una voidable consequence will be the formation of an "external disk" whose outer edge continually moves to larger radii due to angular momentum transport and lack of a confining torque. For long GRBs, if the mass distribution in the initial fall-back disk traces the progenitor envelope, then a radius approximates 10(exp 11) cm gives a time scale app roximately 10(exp 4) s for the X-ray plateau. For late times t > 10(exp 7) s a steepening due to a cooling front in the disk may have obser vational support in GRB 060729. For short GRBs, one expects most of t he mass initially to lie at small radii < 10(exp 8) cm; however the presence of even a trace amount approximately 10(exp -9) M(solar) of hi gh angular material can give a brief plateau in the light curve.

Measurements of Heat Transfer and Boundary-Layer Transition on an 8-Inch-Diameter Hemisphere-Cylinder in Free Flight for a Mach Number Range of 2.00 to 3.88

NASA Technical Reports Server (NTRS)

Garland, Benjamine J.; Chauvin, Leo T.

1957-01-01

Measurements of aerodynamic heat transfer have been made along the hemisphere and cylinder of a hemisphere-cylinder rocket-propelled model in free flight up to a Mach number of 3.88. The test Reynolds number based on free-stream condition and diameter of model covered a range from 2.69 x l0(exp 6) to 11.70 x 10(exp 6). Laminar, transitional, and turbulent heat-transfer coefficients were obtained. The laminar data along the body agreed with laminar theory for blunt bodies whereas the turbulent data along the cylinder were consistently lower than that predicted by the turbulent theory for a flat plate. Measurements of heat transfer at the stagnation point were, in general, lower than the theory for stagnation-point heat transfer. When the Reynolds number to the junction of the hemisphere-cylinder was greater than 6 x l0(exp 6), the transitional Reynolds number varied from 0.8 x l0(exp 6) to 3.0 x 10(exp 6); however, than 6 x l(exp 6) when the Reynolds number to the junction was less, than the transitional Reynolds number varied from 7.0 x l0(exp 6) to 24.7 x 10(exp 6).

Ultra-Sensitive Transition-Edge Sensors for the Background Limited Infrared/Sub-mm Spectrograph (BLISS)

NASA Technical Reports Server (NTRS)

Beyer, A. D.; Kenyon, M. E.; Echternach, P. M.; Chui, T.; Eom, B.-H.; Day, P. K.; Bock, J. J.; Holmes, W.A.; Bradford, C. M.

2011-01-01

We report progress in fabricating ultra-sensitive superconducting transition-edge sensors (TESs) for BLISS. BLISS is a suite of grating spectrometers covering 35-433 micron with R approx. 700 cooled to 50 mK that is proposed to fly on the Japanese space telescope SPICA. The detector arrays for BLISS are TES bolometers readout with a time domain SQUID multiplexer. The required noise equivalent power (NEP) for BLISS is NEP = 10(exp -19) W/Hz(exp 1/2) with an ultimate goal of NEP= 5 x 10(exp -20) W/Hz(exp 1/2) to achieve background limited noise performance. The required and goal response times are tau = 150 ms and tau = 50ms respectively to achieve the NEP at the required and goal optical chop frequency 1-5 Hz. We measured prototype BLISS arrays and have achieved NEP = 6 x 10(exp -18) W/Hz(exp 1/2) and tau = 1.4 ms with a Ti TES (T(sub C) = 565 mK) and NEP approx. 2.5 x 10(exp -19) W/Hz(exp 1/2) and tau approximates 4.5 ms with an Ir TES (T(sub C) = 130 mK). Dark power for these tests is estimated at 1-5 fW.

A New Classification of the Dictyostelids.

PubMed

Sheikh, Sanea; Thulin, Mats; Cavender, James C; Escalante, Ricardo; Kawakami, Shin-Ichi; Lado, Carlos; Landolt, John C; Nanjundiah, Vidyanand; Queller, David C; Strassmann, Joan E; Spiegel, Frederick W; Stephenson, Steven L; Vadell, Eduardo M; Baldauf, Sandra L

2018-02-01

Traditional morphology-based taxonomy of dictyostelids is rejected by molecular phylogeny. A new classification is presented based on monophyletic entities with consistent and strong molecular phylogenetic support and that are, as far as possible, morphologically recognizable. All newly named clades are diagnosed with small subunit ribosomal RNA (18S rRNA) sequence signatures plus morphological synapomorphies where possible. The two major molecular clades are given the rank of order, as Acytosteliales ord. nov. and Dictyosteliales. The two major clades within each of these orders are recognized and given the rank of family as, respectively, Acytosteliaceae and Cavenderiaceae fam. nov. in Acytosteliales, and Dictyosteliaceae and Raperosteliaceae fam. nov. in Dictyosteliales. Twelve genera are recognized: Cavenderia gen. nov. in Cavenderiaceae, Acytostelium, Rostrostelium gen. nov. and Heterostelium gen. nov. in Acytosteliaceae, Tieghemostelium gen. nov., Hagiwaraea gen. nov., Raperostelium gen. nov. and Speleostelium gen. nov. in Raperosteliaceae, and Dictyostelium and Polysphondylium in Dictyosteliaceae. The "polycephalum" complex is treated as Coremiostelium gen. nov. (not assigned to family) and the "polycarpum" complex as Synstelium gen. nov. (not assigned to order and family). Coenonia, which may not be a dictyostelid, is treated as a genus incertae sedis. Eighty-eight new combinations are made at species and variety level, and Dictyostelium ammophilum is validated. Copyright © 2017 Elsevier GmbH. All rights reserved.

Benzoate Metabolism Intermediate Benzoyl Coenzyme A Affects Gentisate Pathway Regulation in Comamonas testosteroni

PubMed Central

Chen, Dong-Wei; Zhang, Yun; Jiang, Cheng-Ying

2014-01-01

A previous study showed that benzoate was catabolized via a coenzyme A (CoA)-dependent epoxide pathway in Azoarcus evansii (R. Niemetz, U. Altenschmidt, S. Brucker, and G. Fuchs, Eur. J. Biochem. 227:161-168, 1995), but gentisate 1,2-dioxygenase was induced. Similarly, we found that the Comamonas testosteroni strain CNB-1 degraded benzoate via a CoA-dependent epoxide pathway and that gentisate 1,2-dioxygenase (GenA) was also induced when benzoate or 3-hydroxybenzoate served as a carbon source for growth. Genes encoding the CoA-dependent epoxide (box genes) and gentisate (gen genes) pathways were identified. Genetic disruption revealed that the gen genes were not involved in benzoate and 3-hydroxybenzoate degradation. Hence, we investigated gen gene regulation in the CNB-1 strain. The PgenA promoter, a MarR-type regulator (GenR), and the GenR binding site were identified. We found that GenR took gentisate, 3-hydroxybenzoate, and benzoyl-CoA as effectors and that binding of GenR to its target DNA sequence was prohibited when these effectors were present. In vivo studies showed that the CNB-1 mutant that lost benzoyl-CoA synthesis was not able to activate PgenA promoter, while transcription of genA was upregulated in another CNB-1 mutant that lost the ability to degrade benzoyl-CoA. The finding that benzoyl-CoA (a metabolic intermediate of benzoate degradation) and 3-hydroxybenzoate function as GenR effectors explains why GenA was induced when CNB-1 grew on benzoate or 3-hydroxybenzoate. Regulation of gentisate pathways by MarR-, LysR-, and IclR-type regulators in diverse bacterial groups is discussed in detail. PMID:24771026

Time Correlations and the Frequency Spectrum of Sound Radiated by Turbulent Flows

NASA Technical Reports Server (NTRS)

Rubinstein, Robert; Zhou, Ye

1997-01-01

Theories of turbulent time correlations are applied to compute frequency spectra of sound radiated by isotropic turbulence and by turbulent shear flows. The hypothesis that Eulerian time correlations are dominated by the sweeping action of the most energetic scales implies that the frequency spectrum of the sound radiated by isotropic turbulence scales as omega(exp 4) for low frequencies and as omega(exp -3/4) for high frequencies. The sweeping hypothesis is applied to an approximate theory of jet noise. The high frequency noise again scales as omega(exp -3/4), but the low frequency spectrum scales as omega(exp 2). In comparison, a classical theory of jet noise based on dimensional analysis gives omega(exp -2) and omega(exp 2) scaling for these frequency ranges. It is shown that the omega(exp -2) scaling is obtained by simplifying the description of turbulent time correlations. An approximate theory of the effect of shear on turbulent time correlations is developed and applied to the frequency spectrum of sound radiated by shear turbulence. The predicted steepening of the shear dominated spectrum appears to be consistent with jet noise measurements.

Rosat detections of X-ray emission from young B-type stars

NASA Technical Reports Server (NTRS)

Schmitt, J. H. M. M.; Zinnecker, H.; Cruddace, R.; Harnden, F. R., Jr.

1993-01-01

We present first results of a series of pointings of the Rosat HRI at visual binaries consisting of a B-star with a later-type companion. The binaries selected for this study are very likely physical pairs. Dating of the B-type stars with respect to the zero-age main sequence, as well as spectroscopic observations of the late-type stars, provides evidence for the extreme youth of these systems with ages typically near or below 10 exp 8 yr. Surprisingly, the late-B component was in many cases detected as an X-ray source, in contrast to previous findings that X-ray emission among late-B field stars is rather uncommon.

The effect of nitrate and monensin on in vitro ruminal fermentation.

PubMed

Capelari, M; Powers, W

2017-11-01

Two experiments evaluated the effect of calcium ammonium nitrate decahydrate (calcium nitrate [NIT]) and monensin sodium (MON) on in vitro fermentation parameters of 2 contrasting diets (100:0 and 10:90 forage-to-concentrate ratios). Diet addition of NIT (0, 1.25, and 2.5 g/100 g DM) and MON (0, 3, and 6 mg/L) were tested alone and combined (9 treatments total; 5 bottles per treatment). Mixed ruminal microorganisms were incubated in anaerobic media containing 0.5 g of substrate diet, 1 of 9 treatments, and 40 mL buffer solution. Incubations were performed in batch cultures for 48 h at 39°C. Headspace gas volume was measured and sampled at 4, 8, 12, 24, and 48 h, and the VFA profile was assessed at the end of the experiment. Total gas production was reduced by NIT (87.9 vs. 94.6 mL; < 0.01) and MON (78.6 vs. 94.6 mL; < 0.01) and, in Exp. 2, further reduced by NIT+MON when the additives were combined (161.1 vs. 196.9 mL; < 0.01). Methane production from control in Exp. 1 and Exp. 2 averaged 9.1 and 15.3 mL, respectively, and was decreased by NIT (3.4 and 8.3 mL in Exp. 1 and Exp. 2, respectively; P < 0.01), MON (4.1 and 7.7 mL; in Exp. 1 and Exp. 2, respectively; < 0.01) and NIT+MON (1.1 and 1.5 mL; in Exp. 1 and Exp. 2, respectively; < 0.01). Both experiments demonstrated a significant increase in nitrous oxide (NO; < 0.01) when NIT was added. Compared to the control treatment, IVDMD was reduced when NIT+MON was added at the higher doses in EXP1 (31.7 vs. 37.4%; < 0.01) and EXP2 (76.6 vs. 79.9 %; < 0.01). Net VFA production was not affected by treatments ( > 0.10), but molar proportions of acetate and butyrate were reduced by MON ( < 0.01). Propionate molar proportion was increased in both experiments by MON ( < 0.01) and further increased in Exp. 2 when the additives were combined at lower doses ( < 0.01). Compared to the control treatment, the acetate:propionate (A:P) ratio was reduced by MON in Exp. 1(1.2 vs. 2.8; < 0.01) and Exp. 2 (1.0 vs. 2.3; < 0.01). Fermentation efficiency (%) was increased by MON (81.7 vs. 73.7%; < 0.01) and further increased in Exp. 2 when the additives were combined at lower doses (87.2 vs. 76.6%; < 0.01). The combination of NIT and MON in 2 contrasting diets proved beneficial by altering fermentation products toward lower CH and more propionate; however, the addition of NIT consistently increased NO production. Negative effects of the additives on IVDMD were found only when the additives were combined at higher doses.

77 FR 54648 - Seventh Meeting: RTCA NextGen Advisory Committee (NAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-05

...' license/State-issued ID Card Number and State of Issuance Company Phone number contact Non-U.S. Citizens... can be used for NextGen Metrics Data Sources for Measuring NextGen Fuel Impact A discussion of a preliminary report on a critical data source to track and analyze the impact of NextGen Non-Technical Barriers...

TideGen LCOE Workbooks

DOE Data Explorer

Jarlath McEntee

2016-03-21

Workbooks showing Annualized Energy Production, Cost Breakdown Structure, Levelized Cost of Electricity for DOE Refernce Tidal Project 1) Baseline TidGen Power System 2) TidGen Power System with the application of Advanced Controls 3) Advanced TidGen Power System with several enhancements These files are provided as a zipped set. Files are linked together and must be viewed in the same folder.

Optical interferometer in space

NASA Technical Reports Server (NTRS)

Bender, Peter L.; Faller, J. E.; Hall, J. L.; Hils, D.; Stebbins, R. T.; Vincent, Mark A.

1989-01-01

The present design concepts for a Laser Gravitational Wave Observatory in Space are described. Laser heterodyne distance measurements are made between test masses located in three spacecraft separated by roughly 10(exp 6) km. The major technology issues are: the reduction of spurious acceleration noise for the test masses to below 2 x 10(exp -15) cm/sq sec/Hz(0.5) from 10(exp -5) to 10(exp -3) Hz; and the measurement of changes in the difference of the antenna arm lengths to 5 x 10(exp -11) cm/Hz(0.5) from 10(exp -3) to 1 Hz with high reliability. The science objectives are: to measure discrete sinusoidal gravitational wave signals from individual sources with periods of 1 second to 1 day; to measure the stochastic background due to unresolved binaries; and to search for gravitational wave pulses with periods longer than 1 sec from possible exotic sources such as gravitational collapse of very massive objects.

Thermochemistry and Kinetics of the Cl+O2 Association Reaction

NASA Technical Reports Server (NTRS)

Nicovich, J. M.; Kreutter, K. D.; Shackelford, C. J.; Wine, P. H.

1997-01-01

Laser flash photolysis of Cl2/O2 mixtures has been employed in conjunction with Cl((sup 2)P(sub 3/2)) detection by time-resolved fluorescence spectroscopy to investigate equilibration kinetics for the reactions Cl + O2 + O is in equilibrium with ClOO + O2 at temperatures of 181-200 K and O2 pressures of 15-40 Torr. The third-order rate coefficient for the association reaction at 186.5 +/- 5.5 K is (8.9 +/- 2.9) x 10(exp -33) cm(exp 6) molecule(exp -2) s(exp -1) and the equilibrium constant (K(p)) at 185.4 K is 18.9 atm(exp -1) (factor of 1.7 uncertainty). A third law analysis of our data leads to a value for the Cl-OO bond dissociation energy of 4.76 +/- 0.49 kcal mol(exp -1).

The microgravity environment of the Space Shuttle Columbia payload bay during STS-32

NASA Technical Reports Server (NTRS)

Dunbar, Bonnie J.; Giesecke, Robert L.; Thomas, Donald A.

1991-01-01

Over 11 hours of three-axis microgravity accelerometer data were successfully measured in the payload bay of Space Shuttle Columbia as part of the Microgravity Disturbances Experiment on STS-32. These data were measured using the High Resolution Accelerometer Package and the Aerodynamic Coefficient Identification Package which were mounted on the Orbiter keel in the aft payload bay. Data were recorded during specific mission events such as Orbiter quiescent periods, crew exercise on the treadmill, and numerous Orbiter engine burns. Orbiter background levels were measured in the 10(exp -5) G range, treadmill operations in the 10(exp -3) G range, and the Orbiter engine burns in the 10(exp -2) G range. Induced acceleration levels resulting from the SYNCOM satellite deploy were in the 10 (exp -2) G range, and operations during the pre-entry Flight Control System checkout were in the 10(exp -2) to 10(exp -1) G range.

Radio Detections During Two State Transitions of the Intermediate-Mass Black Hole HLX-1

NASA Technical Reports Server (NTRS)

Webb, Natalie; Cseh, David; Lenc, Emil; Godet, Olivier; Barret, Didier; Corbel, Stephane; Farrell, Sean; Fender, Robert; Gehrels, Neil; Heywood, Ian

2012-01-01

Relativistic jets are streams of plasma moving at appreciable fractions of the speed of light. They have been observed from stellar-mass black holes (approx. 3 to 20 solar masses) as well as supermassive black holes (approx.. 10(exp 6) to 10(exp 9) Solar Mass) found in the centers of most galaxies. Jets should also be produced by intermediate-mass black holes (approx. 10(exp 2) to 10(exp 5) Solar Mass), although evidence for this third class of black hole has, until recently, been weak. We report the detection of transient radio emission at the location of the intermediate-mass black hole candidate ESO 243-49 HLX-1, which is consistent with a discrete jet ejection event. These observations also allow us to refine the mass estimate of the black hole to be between approx. 9 × 10(exp 3) Solar Mass and approx. 9 × 10(exp 4) Solar Mass.

The Chronology of Asteroid Accretion, Differentiation, and Secondary Mineralization

NASA Technical Reports Server (NTRS)

Nyquist, L. E.; Kleine, T.; Shih, C.-Y.; Reese, Y. D.

2008-01-01

We evaluate initial (Al-26/Al-27)(sub I), (Mn-53/Mn-55)(sub I), (Hf-182/Hf-180)(sub I), and Pb-207/Pb-206 ages for igneous differentiated meteorites and chondrules from ordinary chondrites for consistency with radioactive decay of the parent nuclides within a common, closed isotopic system, i.e., the early solar nebula. We find that the relative abundances of Al-26, Mn-53, and Hf-182, here denoted by I(Al)(sub CAI, I(Mn)(sub CAI) and I(Hf)(sub CAI), are consistent with decay from common initial values for the bulk solar system. I(Mn)(sub CAI) and I(Hf)(sub CAI) = 9.1+/-1.7 x 10(exp -6) and 1.06+/-0.09 x 10(exp -6) respectively, correspond to the canonical value of I(Al)(sub CAI) = 5.1 x 10(exp -5). I(Hf)(sub CAI) thus determined is consistent with I(Hf)(sub CAI) = 1.003+/-0.045 x 10(exp -6) directly determined in separate work. I(Mn)(sub CAI) is within error of the lowest value directly determined for CAI. We suggest that erratically higher values directly determined for CAI in carbonaceous chondrites reflect proton irradiation of unaccreted CAIs by the early Sun after other asteroids destined for melting by Al-26 decay had already accreted. The Mn-53 incorporated within such asteroids would have been shielded from further "local" spallogenic contributions. The relative abundances of the short-lived nuclides are less consistent with the Pb-207/Pb-206 ages of the corresponding materials with the best consistency being obtained between (Hf-182/Hf-180)(sub I) and Pb-207/Pb-206 ages of angrites. (Hf-182/Hf-180)(sub I) decreases with decreasing Pb-207/Pb-206 ages at the rate expected from the 8.90+/-0.09 Ma half-life of Hf-182. However, the model "CAI age" thus determined, T(sub CAI,Mn-W) = 4568.6+/-0.7 Ma, is older than the commonly accepted directly measured value T(sub CAI) = 4567.l+/-0.2 Ma. I(Al)(sub I), and (Mn-53/Mn-55)(sub I) are less consistent with Pb-207/Pb-206 ages, but determine T(sub CAI, Mn-Cr) = 4568.3+/-0.5 Ma relative to I(AI)(sub CAI)= 5.1 x 10(exp -5) and a Pb-207/Pb-206 age of 4558.6 Ma for the LEW86010 angrite. However. the (Mn-53/Mn-55)(sub I) and Pb-207/Pb-206 ages of "intermediate" age D'Orbigny-clan angrites and Asuka 881394 are inconsistent with radioactive decay from CAI values with a Mn-55 half-life of 3.7+/-0.4 Ma. in spite of consistency between (Mn-53/Mn-55)(sub I) and (Al-26/Al-27)(sub I). Nevertheless, it appears that the Mn-Cr method with I(Mn)(sub CAI) = 9.1+/-1.7 x 10(exp -6) can be used to date primary igneous events and also secondary mineralization on asteroid parent bodies. We summarize ages thus determined for igneous events on differentiated asteroids and for carbonate and fayalite formation on carbonaceous asteroids.

Use of a commercial probiotic supplement in meat goats.

PubMed

Whitley, N C; Cazac, D; Rude, B J; Jackson-O'Brien, D; Parveen, S

2009-02-01

Sixty-three Boer crossbred goats were used in 5 separate experiments (Exp. 1 to 5) to evaluate the effects of a commercial probiotic supplement on growth performance (Exp. 1 to 4), diet digestibility (Exp. 5), carcass traits (Exp. 3), and fecal bacterial populations (Exp. 4). Goats were either fed a commercially pelleted concentrate diet and supplemented with a commercial probiotic (PRO) that had shown anecdotal positive effects on goat growth and performance according to local goat producers, or they remained as controls. The dose of PRO used was within the labeled dose for sheep for all studies. For Exp. 1, goat BW and feed intake were measured and G:F was calculated every 7 d for 56 d. For Exp. 2 to 4, BW and feed intake were measured and G:F was calculated every 14 d. The first day of supplementation was considered d 0. Carcass traits were also collected at slaughter on d 57 for Exp. 3, and fecal samples were collected every 14 d for microbial culture for Exp. 4. For Exp. 5, which was a digestibility trial that lasted for 10 d, animals were placed in metabolic pens for collection of feces and orts. Growth performance of goats was not affected by probiotic supplementation, with the exception of performance in Exp. 2, in which ADG and G:F were improved (P < 0.03) in PRO goats compared with control goats on d 56 only (treatment x day interaction; P < 0.05), averaging 0.21 +/- 0.02 kg/d for PRO goats and 0.11 +/- 0.02 kg/d for control goats for ADG and 0.17 +/- 0.02 for PRO goats and 0.10 +/- 0.02 for control goats for G:F. Carcass weights and weights of fabricated cuts (shoulder, loin, leg, rack, shank, and total parts) as well as carcass length, leg circumference, loin eye area, and backfat were not influenced by PRO supplementation. Apparent digestibilities of OM, DM, NDF, ADF, CP, and GE (on a DM basis) were similar for the PRO and control treatments. Fecal culture analysis of Escherichia coli and coliforms, Lactobacillus, and Bifidobacterium populations were not influenced by the PRO treatment. Overall, although the PRO treatment affected goat ADG and G:F in Exp. 2, no PRO treatment effects were noted on growth performance for Exp. 1, 3, and 4. Furthermore, the PRO treatment did not affect diet digestibility, carcass traits, or fecal microbial populations in goats. In conclusion, no consistent benefits were noted from supplementing healthy, growing meat goats with PRO.

Effects of reduced dietary energy and amino acid density on Pectoralis major myopathies in broiler chickens at 36 and 49 days of age1.

PubMed

Meloche, K J; Fancher, B I; Emmerson, D A; Bilgili, S F; Dozier, W A

2018-05-01

Two experiments (Exp) were conducted to determine if reductions in the incidence and severity of wooden breast (WB) and white striping (WS) may be obtained by reducing dietary nutrient density. In each Exp, Yield Plus × Ross 708 male broiler chicks were placed into 63 pens (22 birds/pen). All birds received an identical prestarter diet until 7 d of age, after which time each pen was randomly assigned to 1 of the following 7 dietary treatments (TRT) for the starter (8 to 14 d), grower (15 to 24 d), finisher 1 (Exp 1: 26 to 35 d; Exp 2: 26 to 42 d), and withdrawal (Exp 2: 43 to 48 d) phases: 1) 100% of primary breeder recommendations for digestible amino acid and metabolizable energy density throughout Exp; 2) 95% of TRT 1 until 14 d of age, then as TRT 1; 3) 95% of TRT 1 until 24 d of age, then as TRT 1; 4) 95% of TRT 1 throughout Exp; 5) 90% of TRT 1 until 14 d of age, then as TRT 1; 6) 90% of TRT 1 until 24 d of age, then as TRT 1; 7) 90% of TRT 1 throughout Exp. At 36 d (Exp 1) and 49 d (Exp 2), 18 birds per pen were processed and evaluated for WS and WB. In Exp 1, reduced dietary density in the starter phase (TRT 2 and TRT 5) resulted in increased (P ≤ 0.05) incidences of severe WB (32.9% and 34.7%) relative to TRT 1 (18.2%). In Exp 2, broilers assigned to TRT 7 had reduced (P < 0.01) incidences of severe WB (20.8%) and WS (42.3%) relative to the control (WB: 36.5%; WS: 64.5%). In both Exp, plasma creatine kinase and lactate dehydrogenase increased (P ≤ 0.05) with increasing scores for WB and WS. Reducing dietary nutrient density from 8 to 14 d may exacerbate fillet myopathies in broilers reared to 35 d of age. Although reducing dietary energy and amino acid density to 90% of recommendations from 1 to 48 d reduced the severity of myopathies, these reductions occurred with compromises in live performance. Altogether, these results indicated that concurrent manipulation of dietary amino acid and energy density is not a viable practical solution for breast myopathies.

Low LBNP Tolerance in Men is Associated With Attenuated Activation of The Renin-Angiotensin System

NASA Technical Reports Server (NTRS)

Greenleaf, J. E.; Petersen, T. W.; Gabrielsen, A.; Pump, B.; Bie, P.; Christensen, N.-J.; Warberg, J.; Videbaeck, R.; Simonson, S. R.; Norsk, P.

1999-01-01

Vasoactive hormone concentrations [epinephrine (pE), norepinephrine (pNE), angiotensin II (pATII), vasopressin (pVP), endothelin 1 (pET1)] and plasma renin activity (pRA) were measured during lower body negative pressure (LBNP) to test the hypothesis that responsiveness of the renin-angiotensin system is related to LBNP tolerance. Healthy men (2,822 cal/day(exp -1), 2 mmol*kg(exp -1)*day(exp -1)) Na(+)) were exposed to 30 minutes of progressive LBNP to -50 mmHg. LBNP was uneventful for seven men (25 +/- 2 years, HiTol group), but eight men (26 +/- 3 years) reached pre-syncope after 11 +/- 1 minutes (P < 0.001, LoTol group). Mean arterial pressure was unchanged. Central venous pressure and left atrial diameter decreased in both groups (5-6 mmHg by approx. 30%, P < 0.05). Control [hormone] were similar but, pRA differed between groups (LoTol 0.6 +/- 0.1, HiTol 1.2 +/- 0.1 ng Ang1/(ml(exp -1)*h(exp -1)), P < 0.05). LBNP increased (P < 0.05) pRA and pATII more in HiTol (9.9 +/- 2.2 ng Ang1/(ml(exp -1)*h(exp -1)) and 58 +/- 12 pg/ml(exp -1)) than LoTol (4.3 +/- 0.9 ng Ang1/(ml*h) and 28 +/- 6 pg/ml(exp -1)). In contrast, pVP was higher (P < 0.05) in LoTol than in HiTol. The response of the renin-angiotensin system seems linked to the occurrence of pre-syncope, and measurement of resting pRA may be predictive.

Expression and characterization of the Plasmodium translocon of the exported proteins component EXP2.

PubMed

Hakamada, Kazuaki; Watanabe, Hirokazu; Kawano, Ryuji; Noguchi, Keiichi; Yohda, Masafumi

2017-01-22

The malaria parasite Plasmodium falciparum requires the Plasmodium translocon of exported proteins (PTEX) to proliferate in human red blood cells. During the blood stages of malaria, several hundred parasite-encoded proteins are exported from the parasite into the cytosol of red blood cells. PTEX is the translocon for protein export and comprises 5 proteins: EXP2, PTEX150, PTEX88, Hsp101 and TRX2. Among them, EXP2 is thought to constitute the transmembrane pore, whereas the other components seem to play a role in unfolding the luggage proteins or providing a driving force. However, detailed functional and structural characterizations of PTEX proteins have not been performed. In this study, we expressed and characterized the membrane-associated component EXP2. Because expression of EXP2 is lethal to E. coli, EXP2 was expressed as a fusion protein with GST, and the recombinant EXP2 was obtained by protease digestion. The recombinant EXP2 formed pores in bilayer lipid membranes. The inner diameter of the pore was estimated to be approximately 3.5 nm based on electron microscopy images and channel currents. From this size and the molecular mass as determined by size exclusion chromatography and blue native polyacrylamide gel electrophoresis, we determined that the pore comprises approximately 10-12 EXP2 subunits. However, there is a possibility that the pore structure is different in the PTEX complex. These results provide important insights in the protein transport mechanism of PTEX, which will aid in developing new drugs targeting PTEX. Copyright © 2016 Elsevier Inc. All rights reserved.

Thermophysical Properties Along Curiosity's Traverse in Gale Crater, Mars, Derived from the REMS Ground Temperature Sensor

NASA Technical Reports Server (NTRS)

Vasavada, Ashwin R.; Piqueux, Sylvain; Lewis, Kevin W.; Lemmon, Mark T.; Smith, Michael Doyle

2016-01-01

The REMS instrument onboard the Mars Science Laboratory rover, Curiosity, has measured ground temperature nearly continuously at hourly intervals for two Mars years. Coverage of the entire diurnal cycle at 1 Hz is available every few martian days. We compare these measurements with predictions of surface atmosphere thermal models to derive the apparent thermal inertia and thermally derived albedo along the rovers traverse after accounting for the radiative effects of atmospheric water ice during fall and winter, as is necessary to match the measured seasonal trend. The REMS measurements can distinguish between active sand, other loose materials, mudstone, and sandstone based on their thermophysical properties. However, the apparent thermal inertias of bedrock dominated surfaces [approx. 350-550 J m(exp. -2) K(exp. -1 s(exp. -1/2 )] are lower than expected. We use rover imagery and the detailed shape of the diurnal ground temperature curve to explore whether lateral or vertical heterogeneity in the surface materials within the sensor footprint might explain the low inertias. We find that the bedrock component of the surface can have a thermal inertia as high as 650-1700 J m(exp. -2) K(exp. -1) s(exp. -1/2) for mudstone sites and approx. 700 J m(exp. -2) K(exp. -1) s(exp. - 1/2) for sandstone sites in models runs that include lateral and vertical mixing. Although the results of our forward modeling approach may be non-unique, they demonstrate the potential to extract information about lateral and vertical variations in thermophysical properties from temporally resolved measurements of ground temperature.

eXTP: Enhanced X-Ray Timing and Polarimetry Mission

NASA Technical Reports Server (NTRS)

Zhang, S. N.; Feroci, M.; Santangelo, A.; Dong, Y. W.; Feng, H.; Lu, F. J.; Nandra, K.; Wang, Z. S.; Zhang, S.; Bozzo, E.;

The Far-Infrared Spectrum of Arp 220

NASA Technical Reports Server (NTRS)

Gonzalez-Alfonso, Eduardo; Smith, Howard A.; Fischer, Jacqueline; Cernicharo, Jose

2005-01-01

ISO/LWS grating observations of the ultraluminous infrared galaxy Arp 220 shows absorption in molecular lines of OH, H(sub 2)O, CH, NH, and NH(sub 3), as well as in the [O I] 63 micron line and emission in the [C II] 158 micron line. We have modeled the continuum and the emission/absorption of all observed features by means of a non-local radiative transfer code. The continuum from 25 to 1300 microns is modeled as a warm (106 K) nuclear region that is optically thick in the far-infrared, attenuated by an extended region (size 2") that is heated mainly through absorption of nuclear infrared radiation. The molecular absorption in the nuclear region is characterized by high excitation due to the high infrared radiation density. The OH column densities are high toward the nucleus (2 - 6 x 10(exp 17) cm(exp -2)) and the extended region (approximately 2 x 10(exp 17) cm(exp -2)). The H(sub 2)O column density is also high toward the nucleus (2 - 10 x 10(exp 17) cm(exp -2)) and lower in the extended region. The column densities in a halo that accounts for the absorption by the lowest lying levels are similar to what are found in the diffuse clouds toward the star forming regions in the Sgr B2 molecular cloud complex near the Galactic Center. Most notable are the high column densities found for NH and NH(sub 3) toward the nucleus, with values of approximately 1.5 x 10(exp 16) cm(exp -2) and approximately 3 x 10(exp 16) cm(exp -2), respectively, whereas the NH(sub 2) column density is lower than approximately 2 x 10(exp 15) cm(exp -2). A combination of PDRs in the extended region and hot cores with enhanced H(sub 2)O photodissociation and a possible shock contribution in the nuclei may explain the relative column densities of OH and H(sub 2)O, whereas the nitrogen chemistry may be strongly affected by cosmic ray ionization. The [C II] 158 micron line is well reproduced by our models and its deficit relative to the CII/FIR ratio in normal and starburst galaxies is suggested to be mainly a consequence of the dominant non-PDR component of far-infrared radiation, although our models alone cannot rule out extinction effects in the nuclei.

Too much carbon in NGC253

NASA Technical Reports Server (NTRS)

Harrison, Andrew; Brand, Peter; Puxley, Phil; Russell, Adrian

1995-01-01

We have observed C(exp 0) across the nucleus of the starburst galaxy NGC253. C(exp 0) is very bright and we find N(C(exp 0))/N(CO) is between 1.5 and 2.3 across the nucleus. Existing PDR models cannot explain the observed C(exp 0) intensity. The size of the observed region seems to rule out 'simultaneous very early time chemistry' across the nucleus. Chemical models in which C(sup 0)/CO is enhanced deep in molecular clouds by large fluxes of cosmic rays also struggle to explain our observations. The most plausible explanation for the observed C(exp 0) is that the C/O elemental ratio is enhanced in NGC253.

FIFI: The MPE Garching/UC Berkeley Far-Infrared Imaging Fabry-Perot Interferometer

NASA Technical Reports Server (NTRS)

Geis, Norbert; Genzel, Reinhard; Haggerty, M.; Herrmann, F.; Jackson, J.; Madden, Suzanne C.; Nikola, T.; Poglitsch, Albrecht; Rumitz, M.; Stacey, G. J.

1995-01-01

We describe the performance characteristics of the MPE Garching/UC Berkeley Far-Infrared Imaging Fabry-Perot Interferometer (FIFI) for the Kuiper Airborne Observatory (KAO). The spectrometer features two or three cryogenic tunable Fabry-Perot filters in series giving spectral resolution R of up to 10(exp 5) in the range of 40 microns less than lambda less than 200 microns, and an imaging 5x5 array of photoconductive detectors with variable focal plane plate scale. The instrument works at background limited sensitivity of up to 2 x 10(exp -19) W cm(exp -2) Hz(exp -1/2) per pixel per resolution element at R = 10(exp 5) on the KAO.

Generic revision of the ant subfamily Dorylinae (Hymenoptera, Formicidae)

PubMed Central

Borowiec, Marek L.

2016-01-01

Abstract The generic classification of the ant subfamily Dorylinae is revised, with the aim of facilitating identification of easily-diagnosable monophyletic genera. The new classification is based on recent molecular phylogenetic evidence and a critical reappraisal of doryline morphology. New keys and diagnoses based on workers and males are provided, along with reviews of natural history and phylogenetic relationships, distribution maps, and a list of valid species for each lineage. Twenty-eight genera (27 extant and 1 extinct) are recognized within the subfamily, an increase from 20 in the previous classification scheme. Species classified in the polyphyletic Cerapachys and Sphinctomyrmex prior to this publication are here distributed among 9 and 3 different genera, respectively. Amyrmex and Asphinctanilloides are synonymized under Leptanilloides and the currently recognized subgenera are synonymized for Dorylus. No tribal classification is proposed for the subfamily, but several apparently monophyletic genus-groups are discussed. Valid generic names recognized here include: Acanthostichus (= Ctenopyga), Aenictogiton, Aenictus (= Paraenictus, Typhlatta), Cerapachys (= Ceratopachys), Cheliomyrmex, Chrysapace gen. rev., Cylindromyrmex (= Holcoponera, Hypocylindromyrmex, Metacylindromyrmex), Dorylus (= Alaopone syn. n., Anomma syn. n., Cosmaecetes, Dichthadia syn. n., Rhogmus syn. n., Shuckardia, Sphecomyrmex, Sphegomyrmex, Typhlopone syn. n.), Eburopone gen. n., Eciton (= Camptognatha, Holopone, Mayromyrmex), Eusphinctus gen. rev., Labidus (= Nycteresia, Pseudodichthadia), Leptanilloides (= Amyrmex syn. n., Asphinctanilloides syn. n.), Lioponera gen. rev. (= Neophyracaces syn. n., Phyracaces syn. n.), Lividopone, Neivamyrmex (= Acamatus, Woitkowskia), Neocerapachys gen. n., Nomamyrmex, Ooceraea gen. rev. (= Cysias syn. n.), Parasyscia gen. rev., †Procerapachys, Simopone, Sphinctomyrmex, Syscia gen. rev., Tanipone, Vicinopone, Yunodorylus gen. rev., Zasphinctus gen. rev. (= Aethiopopone syn. n., Nothosphinctus syn. n.). PMID:27559303

Perceptions of clinical utility of an Augmented Reality musical software among health care professionals.

PubMed

Corrêa, Ana Grasielle Dionísio; de Assis, Gilda Aparecida; do Nascimento, Marilena; de Deus Lopes, Roseli

2017-04-01

Augmented Reality musical software (GenVirtual) is a technology, which primarily allows users to develop music activities for rehabilitation. This study aimed to analyse the perceptions of health care professionals regarding the clinical utility of GenVirtual. A second objective was to identify improvements to GenVirtual software and similar technologies. Music therapists, occupational therapists, physiotherapists and speech and language therapist who assist people with physical and cognitive disabilities were enrolled in three focus groups. The quantitative and qualitative data were collected through inductive thematic analysis. Three main themes were identified: the use of GenVirtual in health care areas; opportunities for realistic application of GenVirtual; and limitations in the use of GenVirtual. The registration units identified were: motor stimulation, cognitive stimulation, verbal learning, recreation activity, musicality, accessibility, motivation, sonic accuracy, interference of lighting, poor sound, children and adults. This research suggested that the GenVirtual is a complementary tool to conventional clinical practice and has great potential to motor and cognitive rehabilitation of children and adults. Implications for Rehabilitation Gaining health professional' perceptions of the Augmented Reality musical game (GenVirtual) give valuable information as to the clinical utility of the software. GenVirtual was perceived as a tool that could be used as enhancing the motor and cognitive rehabilitation process. GenVirtual was viewed as a tool that could enhance clinical practice and communication among various agencies, but it was suggested that it should be used with caution to avoid confusion and replacement of important services.

Genistein promotes insulin action through adenosine monophosphate-activated protein kinase activation and p70 ribosomal protein S6 kinase 1 inhibition in the skeletal muscle of mice fed a high energy diet.

PubMed

Arunkumar, Elumalai; Anuradha, Carani Venkatraman

2012-08-01

Genistein (GEN), a soy isoflavone, exerts insulin-sensitizing actions in animals; however, the underlying mechanisms have not been determined. Because GEN is a known activator of adenosine monophosphate-activated protein kinase (AMPK), we hypothesize that GEN activates insulin signaling through AMPK activation. To test this hypothesis, a high fat-high fructose diet (HFFD)-fed mice model of insulin resistance was administered GEN, and the insulin signaling pathway proteins in the skeletal muscle were examined. Hyperglycemia and hyperinsulinemia observed in HFFD-fed mice were significantly lowered by GEN. GEN increased insulin-stimulated tyrosine phosphorylation of insulin receptor-β and insulin receptor substrate (IRS) 1 but down-regulated IRS-1 serine phosphorylation in the skeletal muscle of HFFD-fed mice. Furthermore, GEN treatment improved muscle IRS-1-associated phospatidylinositol-3 kinase expression, phosphorylation of Akt at Ser(473), and translocation of glucose transporter subtype 4. Phosphorylation of AMPK at Thr(172) and acetyl coenzyme A carboxylase (ACC) at Ser(79) was augmented, whereas phosphorylation of p70 ribosomal protein S6 kinase 1 at Thr(389) was significantly decreased after GEN treatment in the skeletal muscle of HFFD-fed mice. These results suggest that GEN might improve insulin action in the skeletal muscle by targeting AMPK. Copyright © 2012 Elsevier Inc. All rights reserved.

Gen-27, a newly synthesized flavonoid, inhibits glycolysis and induces cell apoptosis via suppression of hexokinase II in human breast cancer cells.

PubMed

Tao, Lei; Wei, Libing; Liu, Yishi; Ding, Yang; Liu, Xiuting; Zhang, Xin; Wang, Xiaoping; Yao, Yuyuan; Lu, Jinrong; Wang, Qing; Hu, Rong

2017-02-01

We have previously reported that Gen-27, a newly synthesized flavonoid, exhibits anticancer effects against human colorectal cancer cells. In this study, we investigated the anticancer effects in human breast cancer cell lines and its underlying mechanisms. We demonstrated that Gen-27 inhibited the growth and proliferation of human breast cancer cells in concentration and time-dependent manners. It was found that Gen-27 induced mitochondrial-mediated apoptosis, characterized by the dissipation of mitochondrial membrane potential (ΔΨm), cytochrome c (Cyt c) release from mitochondria to cytosol, activation of caspases and induction of poly (ADP-ribose) polymerase (PARP). In addition, Gen-27 inhibited the glycolysis in human breast cancer cells. After treatment with Gen-27, the expression of HKII was down-regulated, accompanied by weakened interaction of HKII and VDAC. Further research revealed that the induction of mitochondrial apoptosis was associated with the decrease of HKII expression by Gen-27. Finally, in vivo studies demonstrated that Gen-27 significantly suppressed the growth and promoted apoptosis of MDA-MB-231 breast cancer orthotopic tumors with low systemic toxicity. In conclusion, the results showed that Gen-27 had significant anticancer effects against human breast cancer and it may potentially be used as a novel anticancer agent for the treatment of breast cancer. Copyright © 2016. Published by Elsevier Inc.

Improvement in Simulation of Eurasian Winter Climate Variability with a Realistic Arctic Sea Ice Condition in an Atmospheric GCM

NASA Technical Reports Server (NTRS)

Lim, Young-Kwon; Ham, Yoo-Geun; Jeong, Jee-Hoon; Kug, Jong-Seong

2012-01-01

The present study investigates how much a realistic Arctic sea ice condition can contribute to improve simulation of the winter climate variation over the Eurasia region. Model experiments are set up using different sea ice boundary conditions over the past 24 years (i.e., 1988-2011). One is an atmospheric model inter-comparison (AMIP) type of run forced with observed sea-surface temperature (SST), sea ice, and greenhouse gases (referred to as Exp RSI), and the other is the same as Exp RSI except for the sea ice forcing, which is a repeating climatological annual cycle (referred to as Exp CSI). Results show that Exp RSI produces the observed dominant pattern of Eurasian winter temperatures and their interannual variation better than Exp CSI (correlation difference up to approx. 0.3). Exp RSI captures the observed strong relationship between the sea ice concentration near the Barents and Kara seas and the temperature anomaly across Eurasia, including northeastern Asia, which is not well captured in Exp CSI. Lagged atmospheric responses to sea ice retreat are examined using observations to understand atmospheric processes for the Eurasian cooling response including the Arctic temperature increase, sea-level pressure increase, upper-level jet weakening and cold air outbreak toward the mid-latitude. The reproducibility of these lagged responses by Exp RSI is also evaluated.

Global regulators ExpA (GacA) and KdgR modulate extracellular enzyme gene expression through the RsmA-rsmB system in Erwinia carotovora subsp. carotovora.

PubMed

Hyytiäinen, H; Montesano, M; Palva, E T

2001-08-01

The production of the main virulence determinants, the extracellular plant cell wall-degrading enzymes, and hence virulence of Erwinia carotovora subsp. carotovora is controlled by a complex regulatory network. One of the global regulators, the response regulator ExpA, a GacA homolog, is required for transcriptional activation of the extracellular enzyme genes of this soft-rot pathogen. To elucidate the mechanism of ExpA control as well as interactions with other regulatory systems, we isolated second-site transposon mutants that would suppress the enzyme-negative phenotype of an expA (gacA) mutant. Inactivation of kdgR resulted in partial restoration of extracellular enzyme production and virulence to the expA mutant, suggesting an interaction between the two regulatory pathways. This interaction was mediated by the RsmA-rsmB system. Northern analysis was used to show that the regulatory rsmB RNA was under positive control of ExpA. Conversely, the expression of rsmA encoding a global repressor was under negative control of ExpA and positive control of KdgR. This study indicates a central role for the RsmA-rsmB regulatory system during pathogenesis, integrating signals from the ExpA (GacA) and KdgR global regulators of extracellular enzyme production in E. carotovora subsp. carotovora.

Role of an expansin-like molecule in Dictyostelium morphogenesis and regulation of its gene expression by the signal transducer and activator of transcription protein Dd-STATa.

PubMed

Ogasawara, Shun; Shimada, Nao; Kawata, Takefumi

2009-02-01

Expansins are proteins involved in plant morphogenesis, exerting their effects on cellulose to extend cell walls. Dictyostelium is an organism that possesses expansin-like molecules, but their functions are not known. In this study, we analyzed the expL7 (expansin-like 7) gene, which has been identified as a putative target of Dd-STATa, a Dictyostelium homolog of the metazoan signal transducer and activator of transcription (STAT) proteins. Promoter fragments of the expL7 were fused to a lacZ reporter and the expression patterns determined. As expected from the behavior of the endogenous expL7 gene, the expL7/lacZ fusion gene was downregulated in Dd-STATa null slugs. In the parental strain, the expL7 promoter was activated in the anterior tip region. Mutational analysis of the promoter identified a sequence that was necessary for expression in tip cells. In addition, an activator sequence for pstAB cells was identified. These sequences act in combination with the repressor region to prevent ectopic expL7 expression in the prespore and prestalk regions of the slug and culminant. Although the expL7 null mutant showed no phenotypic change, the expL7 overexpressor showed aberrant stalk formation. These results indicate that the expansin-like molecule is important for morphogenesis in Dictyostelium.

Properties Data for Adhesion and Surface Chemistry of Aluminum: Sapphire-Aluminum, Single-Crystal Couple

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa; Pohlchuck, Bobby; Whitle, Neville C.; Hector, Louis G., Jr.; Adams, Jim

1998-01-01

An investigation was conducted to examine the adhesion and surface chemistry of single-crystal aluminum in contact with single-crystal sapphire (alumina). Pull-off force (adhesion) measurements were conducted under loads of 0. I to I mN in a vacuum of 10(exp -1) to 10(exp -9) Pa (approx. 10(exp -10) to 10(exp -11) torr) at room temperature. An Auger electron spectroscopy analyzer incorporated directly into an adhesion-measuring vacuum system was primarily used to define the chemical nature of the surfaces before and after adhesion measurements. The surfaces were cleaned by argon ion sputtering. With a clean aluminum-clean -sapphire couple the mean value and standard deviation of pull-off forces required to separate the surfaces were 3015 and 298 micro-N, respectively. With a contaminated aluminum-clean sapphire couple these values were 231 and 241 micro-N. The presence of a contaminant film on the aluminum surface reduced adhesion by a factor of 13. Therefore, surfaces cleanliness, particularly aluminum cleanliness, played an important role in the adhesion of the aluminum-sapphire couples. Pressures on the order of 10(exp -8) to 10(exp -9) Pa (approx. 10(exp -10) to 10(exp -11) torr) maintained a clean aluminum surface for only a short time (less then 1 hr) but maintained a clean sapphire surface, once it was achieved, for a much longer time.

Accretion Disks Around Binary Black Holes of Unequal Mass: GRMHD Simulations Near Decoupling

NASA Technical Reports Server (NTRS)

Gold, Roman; Paschalidis, Vasileios; Etienne, Zachariah B.; Shapiro, Stuart L.; Pfeiffer, Harald, P.

2013-01-01

We report on simulations in general relativity of magnetized disks onto black hole binaries. We vary the binary mass ratio from 1:1 to 1:10 and evolve the systems when they orbit near the binary disk decoupling radius. We compare (surface) density profiles, accretion rates (relative to a single, non-spinning black hole), variability, effective alpha-stress levels and luminosities as functions of the mass ratio. We treat the disks in two limiting regimes: rapid radiative cooling and no radiative cooling. The magnetic field lines clearly reveal jets emerging from both black hole horizons and merging into one common jet at large distances. The magnetic fields give rise to much stronger shock heating than the pure hydrodynamic flows, completely alter the disk structure, and boost accretion rates and luminosities. Accretion streams near the horizons are among the densest structures; in fact, the 1:10 no-cooling evolution results in a refilling of the cavity. The typical effective temperature in the bulk of the disk is approx. 10(exp5) (M / 10(exp 8)M solar mass (exp -1/4(L/L(sub edd) (exp 1/4K) yielding characteristic thermal frequencies approx. 10 (exp 15) (M /10(exp 8)M solar mass) (exp -1/4(L/L (sub edd) (1+z) (exp -1)Hz. These systems are thus promising targets for many extragalactic optical surveys, such as LSST, WFIRST, and PanSTARRS.

Anomalous Variability in Antarctic Sea Ice Extents During the 1960s With the Use of Nimbus Data

NASA Technical Reports Server (NTRS)

Gallaher, David W.; Campbell, G. Garrett; Meier, Walter N.

2013-01-01

The Nimbus I, II, and III satellites provide a new opportunity for climate studies in the 1960s. The rescue of the visible and infrared imager data resulted in the utilization of the early Nimbus data to determine sea ice extent. A qualitative analysis of the early NASA Nimbus missions has revealed Antarctic sea ice extents that are significant larger and smaller than the historic 1979-2012 passive microwave record. The September 1964 ice mean area is 19.7x10(exp 6) sq. km +/- 0.3x10(exp 6) sq. km. This is more the 250,000 sq. km greater than the 19.44x10(exp 6) sq. km seen in the new 2012 historic maximum. However, in August 1966 the maximum sea ice extent fell to 15.9x10(exp 6) sq. km +/- 0.3x10(exp 6) sq. km. This is more than 1.5x10(exp 6) sq. km below the passive microwave record of 17.5x10(exp 6) sq. km set in September of 1986. This variation between 1964 and 1966 represents a change of maximum sea ice of over 3x10(exp 6) sq. km in just two years. These inter-annual variations while large, are small when compared to the Antarctic seasonal cycle.

GenCade Version 1 Quick-Start Guide: How to Start a Successful GenCade Project

DTIC Science & Technology

2015-03-01

Properly defining the inlets is a crucial part of a GenCade project and can be difficult. A user should become familiar with the Inlet Reservoir Model ( IRM ...GenCade Report 2 provide additional documentation on IRM variable names and functions. 3.9.4 Export data The data may easily be exported to a text

Factors Influencing Retention of Gen Y and Non-Gen Y Teachers Working at International Schools in Asia

ERIC Educational Resources Information Center

Fong, Hoi Wah Benny

2018-01-01

Quantitative studies on international-school teacher retention are few, especially studies that differentiate between Gen Y and non-Gen Y teachers. This article reports on the findings of a study that examined the relationship of job satisfaction factors to the likelihood of contract renewal by international-school teachers. Results from the study…

Head-Worn Displays for NextGen

NASA Technical Reports Server (NTRS)

Bailey, Randall E.; Shelton, Kevin J.; Arthur, J. J.

2011-01-01

The operating concepts emerging under the Next Generation air transportation system (NextGen) require new technology and procedures - not only on the ground-side - but also on the flight deck. Flight deck display and decision support technologies are specifically targeted to overcome aircraft safety barriers that might otherwise constrain the full realization of NextGen. One such technology is the very lightweight, unobtrusive head-worn display (HWD). HWDs with an integrated head-tracking system are being researched as they offer significant potential benefit under emerging NextGen operational concepts. Two areas of benefit for NextGen are defined. First, the HWD may be designed to be equivalent to the Head-Up Display (HUD) using Virtual HUD concepts. As such, these operational credits may be provided to significantly more aircraft for which HUD installation is neither practical nor possible. Second, the HWD provides unique display capabilities, such as an unlimited field-of-regard. These capabilities may be integral to emerging NextGen operational concepts, eliminating safety issues which might otherwise constrain the full realization of NextGen. The paper details recent research results, current HWD technology limitations, and future technology development needed to realize HWDs as a enabling technology for NextGen.

A new strategy for superior reproductive performance of ewes bred out-of-season utilizing progestagen supplement prior to withdrawal of intravaginal pessaries.

PubMed

Husein, M Q; Ababneh, M M

2008-02-01

Two experiments were conducted to examine the effect of progestagen supplement 24h prior to intravaginal pessary withdrawal on reproductive performance of seasonal anestrous ewes. Ewes in each experiment were allocated to treatment and control and all were induced to estrus using either intravaginal MAP (Exp. 1; n=24) or CIDR-G (Exp. 2; n=28) pessaries for 12 days. Half of the ewes in each experiment were supplemented 24h before withdrawal of pessaries with either 10mg oral MAP tablets (Exp. 1) or 25mg i.m. progesterone (P(4)) administration (Exp. 2; P(4)-supplement-treated group). Fertile rams were allowed with the ewes at sponge removal (Day 0, 0h) and estrus was monitored at 6-h intervals for 3 days. Blood samples were collected for measurements of P(4) (Exp. 1 and Exp. 2) and LH (Exp. 2). In both experiments, the percent of ewes in estrus was greater (P<0.05) and intervals to estrus were longer (P<0.05) in progestagen-supplement-treated than control ewes. In Exp. 2, the occurrence and magnitude of LH surges were greater (P<0.01) and intervals to onset of LH surge were longer (P<0.01) in P(4)-supplement-treated than control ewes. In Exp. 2, P(4) supplement elevated P(4) levels from 1.8+/-0.1ng/mL on Day -1 to 4.2+/-0.3 on Day 0 (P<0.001). Following pessaries removal, P(4) concentrations fell to basal values on Day 1 in both groups and remained low until Day 5. Then, P(4) concentrations increased and remained elevated through Day 19 in all (100%) progestagen-supplement-treated in Exp. 1 (12/12) and Exp. 2 (14/14) and in only 5/12 (41.7%) and 6/14 (42.9%) control ewes, respectively. These ewes were confirmed pregnant by ultrasonography and lambed on Day 149.2+/-0.2 following Day 0. In conclusion, progestagen supplement 24h prior to removal of pessary can be used successfully to improve reproductive performance of ewes bred out-of-season.

Digestible lysine requirements of male broilers from 1 to 42 days of age reassessed.

PubMed

Cemin, Henrique Scher; Vieira, Sergio Luiz; Stefanello, Catarina; Kipper, Marcos; Kindlein, Liris; Helmbrecht, Ariane

2017-01-01

Three experiments were conducted separately to estimate the digestible Lys (dig. Lys) requirements of Cobb × Cobb 500 male broilers using different statistical models. For each experiment, 1,200 chicks were housed in 48 floor pens in a completely randomized design with 6 treatments and 8 replicates. Broilers were fed diets with increasing dig. Lys levels from 1 to 12 d (Exp. 1), from 12 to 28 d (Exp. 2), and 28 to 42 d (Exp. 3). Increasing dig. Lys levels were equally spaced from 0.97 to 1.37% in Exp. 1, 0.77 to 1.17% in Exp. 2, and 0.68 to 1.07% in Exp. 3. The lowest dig. Lys diets were not supplemented with L-Lysine and all other essential AA met or exceeded recommendations. In Exp. 3, six birds per pen were randomly selected from each replication to evaluate carcass and breast yields. Digestible Lys requirements were estimated by quadratic polynomial (QP), linear broken-line (LBL), quadratic broken-line (QBL), and exponential asymptotic (EA) models. Overall, dig. Lys requirements varied among response variables and statistical models. Increasing dietary dig. Lys had a positive effect on BW, carcass and breast yields. Levels of dig. Lys that optimized performance using QP, LBL, QBL, and EA models were 1.207, 1.036, 1.113, and 1.204% for BWG and 1.190, 1.027, 1.100, and 1.172% for FCR in Exp. 1; 1.019, 0.853, 0.944; 1.025% for BWG and 1.050, 0.879, 1.032, and 1.167% for FCR in Exp. 2; and 0.960, 0.835, 0.933, and 1.077% for BWG, 0.981, 0.857, 0.963, and 1.146% for FCR in Exp. 3. The QP, LBL, QBL, and EA also estimated dig. Lys requirements as 0.941, 0.846, 0.925, and 1.070% for breast meat yield in Exp. 3. In conclusion, Lys requirements vary greatly according to the statistical analysis utilized; therefore, the origin of requirement estimation must be taken into account in order to allow adequate comparisons between references.

Reconstruction of ionic currents in a molluscan photoreceptor.

PubMed Central

Sakakibara, M.; Ikeno, H.; Usui, S.; Collin, C.; Alkon, D. L.

1993-01-01

Two-microelectrode voltage-clamp measurements were made to determine the kinetics and voltage dependence of ionic currents across the soma membrane of the Hermissenda type B photoreceptor. The voltage-dependent outward potassium currents, IA and ICa(2+)-K+, the inward voltage-dependent calcium current, ICa2+ and the light-induced current, IIgt, were then described with Hodgkin-Huxley-type equations. The fast-activating and inactivating potassium current, IA, was described by the equation; IA(t) = gA(max)(ma infinity[1-exp(-t/tau ma)])3 x (ha infinity [1-exp(-t/tau ha)] + exp(-t/tau ha)) (Vm-EK), where the parameters ma infinity, ha infinity, tau ma, and tau ha are functions of membrane potential, Vm, and ma infinity and ha infinity are steady-state activation and inactivation parameters. Similarly, the calcium-dependent outward potassium current, ICa(2+)-K+, was described by the equation, ICa(2+)-K+ (t) = gc(max)(mc infinity(VC)(1-exp[-t/tau mc (VC)]))pc (hc infinity(VC) [1-exp(-t/tau hc)] + exp(-t/tau hc(VC)])pc(VC-EK). In high external potassium, ICa(2+)-K+ could be measured in approximate isolation from other currents as a voltage-dependent inward tail current following a depolarizing command pulse from a holding potential of -60 mV. A voltage-dependent inward calcium current across the type B soma membrane, ICa2+, activated rapidly, showed little inactivation, and was described by the equation: ICa2+ = gCa(max) [1 + exp](-Vm-5)/7]-1 (Vm-ECa), where gCa(max) was 0.5 microS. The light-induced current with both fast and slow phases was described by: IIgt(t) = IIgt1 + IIgt2 + IIgt3, IIgti = gIgti [1-exp(- ton/tau mi)] exp(-ton/tau hi)(Vm-EIgti) (i = 1, 2). For i = 3, /Igt(t) = gigt3m33h3(Vm - Eigt3)exp(-ton/Ton) x exp(-tfoff/t Off). Based on these reconstructions of ionic currents, learning-induced enhancement of the long lasting depolarization (LLD) of the photoreceptor'slight response was shown to arise from progressive inactivation of /A, lca2+ -K+, and lCa2+. PMID:8369456

Digestible lysine requirements of male broilers from 1 to 42 days of age reassessed

PubMed Central

Cemin, Henrique Scher; Stefanello, Catarina; Kipper, Marcos; Kindlein, Liris; Helmbrecht, Ariane

2017-01-01

Three experiments were conducted separately to estimate the digestible Lys (dig. Lys) requirements of Cobb × Cobb 500 male broilers using different statistical models. For each experiment, 1,200 chicks were housed in 48 floor pens in a completely randomized design with 6 treatments and 8 replicates. Broilers were fed diets with increasing dig. Lys levels from 1 to 12 d (Exp. 1), from 12 to 28 d (Exp. 2), and 28 to 42 d (Exp. 3). Increasing dig. Lys levels were equally spaced from 0.97 to 1.37% in Exp. 1, 0.77 to 1.17% in Exp. 2, and 0.68 to 1.07% in Exp. 3. The lowest dig. Lys diets were not supplemented with L-Lysine and all other essential AA met or exceeded recommendations. In Exp. 3, six birds per pen were randomly selected from each replication to evaluate carcass and breast yields. Digestible Lys requirements were estimated by quadratic polynomial (QP), linear broken-line (LBL), quadratic broken-line (QBL), and exponential asymptotic (EA) models. Overall, dig. Lys requirements varied among response variables and statistical models. Increasing dietary dig. Lys had a positive effect on BW, carcass and breast yields. Levels of dig. Lys that optimized performance using QP, LBL, QBL, and EA models were 1.207, 1.036, 1.113, and 1.204% for BWG and 1.190, 1.027, 1.100, and 1.172% for FCR in Exp. 1; 1.019, 0.853, 0.944; 1.025% for BWG and 1.050, 0.879, 1.032, and 1.167% for FCR in Exp. 2; and 0.960, 0.835, 0.933, and 1.077% for BWG, 0.981, 0.857, 0.963, and 1.146% for FCR in Exp. 3. The QP, LBL, QBL, and EA also estimated dig. Lys requirements as 0.941, 0.846, 0.925, and 1.070% for breast meat yield in Exp. 3. In conclusion, Lys requirements vary greatly according to the statistical analysis utilized; therefore, the origin of requirement estimation must be taken into account in order to allow adequate comparisons between references. PMID:28636626

Chromic oxide and acid-insoluble ash as markers in digestibility studies with growing pigs and sows.

PubMed

Brestenský, M; Nitrayová, S; Heger, J; Patráš, P

2017-02-01

The results of three experiments, focused on the determination of endogenous ileal flow (EIF) of amino acids (AA) and nitrogen (N) (Exp. 1), apparent ileal digestibility (AID) of AA and N (Exp. 2), and apparent total tract digestibility (ATTD) of dry matter (DM), organic matter (OM), N, calcium (Ca) and phosphorus (P) (Exps. 2 and 3), were used to compare chromic oxide (Cr 2 O 3 ) and acid-insoluble ash (AIA) as digestibility markers. In Exps. 1 and 2, a total of six gilts fitted with T-cannula in terminal ileum, and in Exp. 3, a total of 24 pregnant sows were used. In Exps. 1 and 2, the pigs were assigned into four dietary treatments according to 4 × 6 crossover design (Exp. 1; diets with 0%, 4%, 8% and 12% of casein; Exp. 2 basal diet with different levels of phytase). In Exp. 3, the sows were assigned to four dietary treatments (basal diet with different levels of phytase) of six sows. In Exps. 1 and 2 ileal digesta and in Exps. 2 and 3 faeces were collected for the determination of EIF, AID and ATTD. Differences in EIF of AA determined by Cr 2 O 3 and AIA ranged (p ˃ 0.05) from -4.62 to 4.54%. The lowest EIF was for methionine and the greatest one for proline, determined by both markers. Apparent ileal digestibility determined by Cr 2 O 3 was slightly greater (p ˃ 0.05) in comparison with AIA. Differences ranged from 1.88% (Arg) to 7.08% (Gly). The greatest AID was for arginine and the lowest one for glycine, determined by both Cr 2 O 3 and AIA. Similarly for ATTD of DM, OM, N, Ca and P, there were no differences in digestibility determined by Cr 2 O 3 and AIA. Both, Cr 2 O 3 and AIA, are suitable and comparable markers for digestibility studies in pigs. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH.

Determining the Covering Factor of Compton-Thick Active Galactic Nuclei with NuSTAR

NASA Technical Reports Server (NTRS)

Brightman, M.; Balokovic, M.; Stern, D.; Arevalo, P.; Ballantyne, D. R.; Bauer, F. E.; Boggs, S. E.; Craig, W. W.; Christensen, F. E.; Zhang, W. W.

2015-01-01

The covering factor of Compton-thick (CT) obscuring material associated with the torus in active galactic nuclei (AGNs) is at present best understood through the fraction of sources exhibiting CT absorption along the line of sight (N(sub H) greater than 1.5 x 10(exp 24) cm(exp -2)) in the X-ray band, which reveals the average covering factor. Determining this CT fraction is difficult, however, due to the extreme obscuration. With its spectral coverage at hard X-rays (greater than 10 keV), Nuclear Spectroscopic Telescope Array (NuSTAR) is sensitive to the AGNs covering factor since Compton scattering of X-rays off optically thick material dominates at these energies. We present a spectral analysis of 10 AGNs observed with NuSTAR where the obscuring medium is optically thick to Compton scattering, so-called CT AGNs. We use the torus models of Brightman and Nandra that predict the X-ray spectrum from reprocessing in a torus and include the torus opening angle as a free parameter and aim to determine the covering factor of the CT gas in these sources individually. Across the sample we find mild to heavy CT columns, with N(sub H) measured from 10(exp 24) to 10(exp 26) cm(exp -2), and a wide range of covering factors, where individual measurements range from 0.2 to 0.9. We find that the covering factor, f(sub c), is a strongly decreasing function of the intrinsic 2-10 keV luminosity, L(sub X), where f(sub c) = (-0.41 +/- 0.13)log(sub 10)(L(sub X)/erg s(exp -1))+18.31 +/- 5.33, across more than two orders of magnitude in L(sub X) (10(exp 41.5) - 10(exp 44) erg s(exp -1)). The covering factors measured here agree well with the obscured fraction as a function of LX as determined by studies of local AGNs with L(sub X) greater than 10(exp 42.5) erg s(exp -1).

GenBank.

PubMed

Benson, Dennis A; Karsch-Mizrachi, Ilene; Lipman, David J; Ostell, James; Wheeler, David L

2007-01-01

GenBank (R) is a comprehensive database that contains publicly available nucleotide sequences for more than 240 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or standalone Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the EMBL Data Library in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI Homepage (www.ncbi.nlm.nih.gov).

GenBank.

PubMed

Benson, Dennis A; Karsch-Mizrachi, Ilene; Lipman, David J; Ostell, James; Wheeler, David L

2005-01-01

GenBank is a comprehensive database that contains publicly available DNA sequences for more than 165,000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or standalone Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the EMBL Data Library in the UK and the DNA Data Bank of Japan helps to ensure worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, go to the NCBI Homepage at http://www.ncbi.nlm.nih.gov.

GenBank.

PubMed

Benson, Dennis A; Karsch-Mizrachi, Ilene; Lipman, David J; Ostell, James; Wheeler, David L

2006-01-01

GenBank (R) is a comprehensive database that contains publicly available DNA sequences for more than 205 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the Web-based BankIt or standalone Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the EMBL Data Library in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, go to the NCBI Homepage at www.ncbi.nlm.nih.gov.

GenBank.

PubMed

Benson, Dennis A; Karsch-Mizrachi, Ilene; Lipman, David J; Ostell, James; Wheeler, David L

2008-01-01

GenBank (R) is a comprehensive database that contains publicly available nucleotide sequences for more than 260 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or standalone Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the European Molecular Biology Laboratory Nucleotide Sequence Database in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI Homepage: www.ncbi.nlm.nih.gov.

GenBank

PubMed Central

Benson, Dennis A.; Karsch-Mizrachi, Ilene; Lipman, David J.; Ostell, James; Wheeler, David L.

2008-01-01

GenBank (R) is a comprehensive database that contains publicly available nucleotide sequences for more than 260 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or standalone Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the European Molecular Biology Laboratory Nucleotide Sequence Database in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI Homepage: www.ncbi.nlm.nih.gov PMID:18073190

GenSSI 2.0: multi-experiment structural identifiability analysis of SBML models.

PubMed

Ligon, Thomas S; Fröhlich, Fabian; Chis, Oana T; Banga, Julio R; Balsa-Canto, Eva; Hasenauer, Jan

2018-04-15

Mathematical modeling using ordinary differential equations is used in systems biology to improve the understanding of dynamic biological processes. The parameters of ordinary differential equation models are usually estimated from experimental data. To analyze a priori the uniqueness of the solution of the estimation problem, structural identifiability analysis methods have been developed. We introduce GenSSI 2.0, an advancement of the software toolbox GenSSI (Generating Series for testing Structural Identifiability). GenSSI 2.0 is the first toolbox for structural identifiability analysis to implement Systems Biology Markup Language import, state/parameter transformations and multi-experiment structural identifiability analysis. In addition, GenSSI 2.0 supports a range of MATLAB versions and is computationally more efficient than its previous version, enabling the analysis of more complex models. GenSSI 2.0 is an open-source MATLAB toolbox and available at https://github.com/genssi-developer/GenSSI. thomas.ligon@physik.uni-muenchen.de or jan.hasenauer@helmholtz-muenchen.de. Supplementary data are available at Bioinformatics online.

Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus.

PubMed

Chen, Fei; Zhu, Liyuan; Zhang, Yiling; Kumar, Dhiraj; Cao, Guangli; Hu, Xiaolong; Liang, Zi; Kuang, Sulan; Xue, Renyu; Gong, Chengliang

2018-05-08

Bombyx mori cypovirus (BmCPV), a member of the Reoviridae, specifically infects silkworms and causes extensive economic losses to the sericulture industry. To date, the entry mechanism of BmCPV into cells is unclear. Here we used electron microscopy to study the route of entry of BmCPV into cells, and the results demonstrated that the entry of BmCPV into BmN cells was mediated by endocytosis. Blocking the entry pathway with four endocytosis inhibitors, including dansylcadaverine, chlorpromazine, genistein, and PP2, significantly decreased the infectivity of BmCPV. This indicates that BmCPV enters BmN cells via endocytosis, and that clathrin-mediated sorting is the predominant entry method. After the relative expression levels of clathrin heavy chain (clathrin, GenBank accession No. NM_001142971.1) and the adaptor protein complex-1 gamma subunit AP-1 (AP-1, GenBank accession No. JQ824201.1), which are involved in clathrin-mediated endocytosis, were inhibited by RNA interference or abolishing the functions of clathrin and AP-1 with their corresponding antibodies, the infectivity of BmCPV was reduced significantly, which suggests that clathrin-mediated endocytosis contributed to the entry of BmCPV into cells. Our findings suggest that the clathrin-mediated endocytosis pathway is a candidate for the development of therapeutics for silkworm cytoplasmic polyhedrosis.

A new genus and three new species of miniaturized microhylid frogs from Indochina (Amphibia: Anura: Microhylidae: Asterophryinae).

PubMed

Poyarkov, Nikolay A; Suwannapoom, Chatmongkon; Pawangkhanant, Parinya; Aksornneam, Akrachai; Duong, Tang Van; Korost, Dmitriy V; Che, Jing

2018-05-18

We report on the discovery of a new genus of microhylid subfamily Asterophryinae from northern and eastern Indochina, containing three new species. Vietnamophryne Gen. nov. are secretive miniaturized frogs (SVL<21 mm) with a mostly semi-fossorial lifestyle. To assess phylogenetic relationships, we studied 12S rRNA-16S rRNA mtDNA fragments with a final alignment of 2 591 bp for 53 microhylid species. External morphology characters and osteological characteristics analyzed using micro-CT scanning were used for describing the new genus. Results of phylogenetic analyses assigned the new genus into the mainly Australasian subfamily Asterophryinae as a sister taxon to the genus Siamophryne from southern Indochina. The three specimens collected from Gia Lai Province in central Vietnam, Cao Bang Province in northern Vietnam, and Chiang Rai Province in northern Thailand proved to be separate species, different both in morphology and genetics (genetic divergence 3.1%≤P≤5.1%). Our work provides further evidence for the "out of Indo-Eurasia" scenario for Asterophryinae, indicating that the initial cladogenesis and differentiation of this group of frogs occurred in the Indochina Peninsula. To date, each of the three new species of Vietnamophryne Gen. nov. is known only from a single specimen; thus, their distribution, life history, and conservation status require further study.

Two new genera of big-eyed minute litter bugs (Hemiptera, Schizopteridae, Hypselosomatinae) from Brazil and the Caribbean

PubMed Central

Hoey-Chamberlain, Rochelle; Weirauch, Christiane

2016-01-01

Abstract Charismatic Hypselosomatinae (currently 14 extant and fossil genera; 72 species), the “big-eyed minute litter bugs”, are characterized among Schizopteridae (Dipsocoromorpha) by the large eyes, four-segmented labium, and distinctive wing venation. A recent molecular phylogenetic analysis confirmed the monophyly of Hypselosomatinae that were recovered as the sister taxon to the Ogeriinae + Schizopterinae (Weirauch and Štys 2014). Hypselosomatinae occur in the Old and New Worlds, but described species diversity is biased towards the Oriental and Australian regions: only three monotypic genera are currently known from the New World (Glyptocombus Heidemann, Ommatides Uhler, and Williamsocoris Carpintero & Dellapé). Based on 28 male, female, and juvenile specimens from Cuba and the Dominican Republic and a single male specimen from Brazil we here describe two new monotypic genera of Hypselosomatinae, Hypselosomops pecki gen. n. and sp. n., and Hypsohapsis takiyae gen. n. and sp. n. We provide habitus images, digital illustrations (light, scanning electron, and/or confocal microscope) of wing and male genitalic structures, line drawings of genitalic structures, and distribution maps. Bizarre morphologies, a worldwide distribution with small endemic species ranges, and a fossil record that dates back to the mid-Cretaceous make the Hypselosomatinae a fascinating group to explore in an effort to understand the evolutionary history of Dipsocoromorpha. PMID:28138288

Analysis Extract. AFSC 4D0X1 Diet Therapy (Active Duty)

DTIC Science & Technology

2002-07-01

Diet (s) exp-Lacto Vegetarian Active Active Active Active AD AD...3* 87* 22* V0143 Diet (s) exp-Lacto-Ovo Vegetarian Diet Active Active... Diet (s) exp-Ovo Vegetarian Active Active Active Active AD AD

Military Standard Generators Prototype Modifications. Volume 3. 60 kW DoD Generator Set

DTIC Science & Technology

1988-03-31

14 JAN ., 17 :35: ’w. 6843 GEN S DS-00 273 . .. •- -,- - 32. 351 3 DE,-.. F Z EX ,-UST 2 895. 93 DEL;. F C REL!:7T 3 988.34 DEC-. F C 4 EXH-4AUST 4...33. 351 DEG. F C 20 GEN. FRAME TOP 11. 64 DEG. F C 2:1 GEN. FRAME BTTM 82. 15± DEG. F C 21 GEN. FRAME BTTM 113. i6 DEG. F C 2,. GEN. EXCITER 32. 992...DEG. F C 21 GEli . FRAME BTTM ±88.15 DEG. F S2 -EN. EXCITER 12. 49 DEG. F C 22 S-EN. EXCITER 185. 5 DEG. F 27 G2N. ’OLT. REG. 95. 459 DEG. F C 23 GEN

Environmental Perturbations Caused by the Impacts of Comets and Asteroids on Earth

NASA Technical Reports Server (NTRS)

Toon, Owen B.; Lawless, James G. (Technical Monitor)

1994-01-01

The extinction mechanisms proposed at the Cretaceous-Tertiary geological boundary are reviewed and related to the impact of asteroids or comets in general. For impact energies below 10(exp 4) Megatons (less than 6 x 10(exp 4) years; asteroid diameter less than 650 m), blast, earthquake, and fire may destroy local areas up to 10(exp 5) square m. Tidal waves could flood a kilometer inland over entire ocean basins. The energy range from 105 to 106 Megatons (less than 2 x 10(exp 6) years; asteroid diameter less than 3 km) is transitional. Dust lifted, sulfur released from within impacting asteroids, and soot from fires started by comets can produce climatologically significant optical depths of 10. At energies beyond 10(exp 7) Megatons, blast and earthquake damage is regional (10(exp 6) square cm). Tsunami cresting to 100 m and flooding 20 km inland will sweep the coastal zones of the world's oceans. Fires will be set globally. Light levels may drop so low from the smoke, dust and sulfate that vision is not possible. At energies approaching 10(exp 9) Megatons the ocean surface waters may be acidified by sulfur. The combination of these effects would be devastating.

Wear-Resistant, Self-Lubricating Surfaces of Diamond Coatings

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa

1995-01-01

In humid air and dry nitrogen, as-deposited, fine-grain diamond films and polished, coarse-grain diamond films have low steady-state coefficients of friction (less than 0.1) and low wear rates (less than or equal to 10(exp -6) mm(exp 3)/N-m). In an ultrahigh vacuum (10(exp -7) Pa), however, they have high steady-state coefficients of friction (greater than 0.6) and high wear rates (greater than or equal to 10(exp -4) mm(exp 3)/N-m). Therefore, the use of as-deposited, fine-grain and polished, coarse-grain diamond films as wear-resistant, self-lubricating coatings must be limited to normal air or gaseous environments such as dry nitrogen. On the other hand, carbon-ion-implanted, fine-grain diamond films and nitrogen-ion-implanted, coarse-grain diamond films have low steady-state coefficients of friction (less than 0.1) and low wear rates (less than or equal to 10(exp -6) mm(exp 3)/N-m) in all three environments. These films can be effectively used as wear-resistant, self-lubricating coatings in an ultrahigh vacuum as well as in normal air and dry nitrogen.

On the Sizes of the North Atlantic Basin Tropical Cyclones Based on 34- and 64-kt Wind Radii Data, 2004-2013

NASA Technical Reports Server (NTRS)

Wilson, Robert M.

2014-01-01

At end of the 2012 hurricane season the National Hurricane Center retired the original HURDAT dataset and replaced it with the newer version HURDAT2, which reformatted the original data and included additional information, in particular, estimates of the 34-, 50, and 64-kt wind radii for the interval 2004-2013. During the brief 10-year interval, some 164 tropical cyclones are noted to have formed in the North Atlantic basin, with 77 becoming hurricanes. Hurricane Sandy (2012) stands out as being the largest individual storm that occurred in the North Atlantic basin during the 2004 -2013 timeframe, both in terms of its 34- and 64-kt wind radii and wind areas, having maximum 34- and 64-kt wind radii, maximum wind areas, and average wind areas each more than 2 standard deviations larger than the corresponding means. In terms of the largest yearly total 34-kt wind area (i.e., the sum of all individual storm 34-kt wind areas during the year), the year 2010 stands out as being the largest (about 423 × 10(exp 6) nmi(exp 2)), compared to the mean of about 174 × 10(exp 6) nmi(exp 2)), surpassing the year 2005 (353 x 10(exp 6) nmi(exp 2)) that had the largest number of individual storms (28). However, in terms of the largest yearly total 64-kt wind area, the year 2005 was the largest (about 9 × 10(exp 6) nmi(exp 2)), compared to the mean of about 3 × 106 nmi(exp 2)). Interesting is that the ratio of total 64-kt wind area to total 34-kt wind area has decreased over time, from 0.034 in 2004 to 0.008 in 2013.

Is strength-training frequency a key factor to develop performance adaptations in young elite soccer players?

PubMed

Otero-Esquina, Carlos; de Hoyo Lora, Moisés; Gonzalo-Skok, Óliver; Domínguez-Cobo, Sergio; Sánchez, Hugo

2017-11-01

The aim of this study was to analyse the effects of a combined strength-training programme (full-back squat, YoYo TM leg curl, plyometrics and sled towing exercises) on performance in elite young soccer players and to examine the effects when this training programme was performed one or two days per week. Thirty-six male soccer players (U-17 to U-19) were recruited and assigned to experimental groups (EXP1: 1 s w -1 ; EXP2: 2 s w -1 ) or a control group (CON). Performance was assessed through a countermovement jump (CMJ) test (relative peak power [CMJ PP ] and CMJ height [CMJ H ]), a 20-m linear sprint test with split-times at 10-m, and a change of direction test (V-cut test) 1 week before starting the training programme and also 1 week after performing such training programme. Within-group analysis showed substantial improvements in CMJ variables (ES: 0.39-0.81) and COD (ES: 0.70 and 0.76) in EXP1 and EXP2, while EXP2 also showed substantial enhancements in all linear sprinting tests (ES: 0.43-0.52). Between-group analysis showed substantially greater improvements in CMJ variables (ES: 0.39-0.68) in experimental groups in comparison to CON. Furthermore, EXP2 achieved a substantial better performance in 20-m (ES: 0.48-0.64) than EXP1 and CON. Finally, EXP2 also showed greater enhancements in 10-m (ES: 0.50) and V-cut test (ES: 0.52) than EXP1. In conclusion, the combined strength-training programme improved jumping ability, independently of training frequency, though the achievement of two sessions per week also enhanced sprinting abilities (linear and COD) in young soccer players.

The Question of Iranian Occupation of the Islands, Greater Tunb, Lesser Tunb and Abu Musa Belonging to the United Arab Emirates

DTIC Science & Technology

1994-05-17

ln r ýIni d le, e- s o Mh ..- rianlM’’ion ’-n cmn,entsr Naor qjai iris vturden Mi~"’ ,e .jr iv )ther aspe(? t )f hi o~e1 2 r’n’~i~.,rUd,’ uqgt~ii~ons...DATES COVERED 117 May 1994 Study Project 4. TITLE AND SUBTITLE S . FUNDING NUMBERS The Question of Iranian Occupation of the Islands, Greater Tunb...Lesser Tunb, and Abu Musa Belonaing to the United Arab Emirates 6. AUTHOR( S ) B.Gen Mohamad Hilal Al-Kaabi United Arab Emirates 7. PERFORMING ORGANIZATION

Panchromatic Observations of SN2011dh Point to a Compact Progenitor Star

NASA Technical Reports Server (NTRS)

Soderberg, A. M.; Margutti, R.; Zauerer, B. A.; Krauss, M.; Katz, B.; Chomiuk, L.; Dittmann, J. A.; Nakar, E.; Sakamoto, T.; Kawai, N.;

Photochemistry of Triton's Atmosphere and Ionosphere

NASA Technical Reports Server (NTRS)

Krasnopolsky, Vladimir A.; Cruikshank, Dale P.

1995-01-01

The photochemistry of 32 neutral and 21 ion species in Triton's atmosphere is considered. Parent species N2, CH4, and CO (with a mixing ratio of 3 x 10(exp -4) in our basic model) sublime from the ice with rates of 40, 208, and 0.3 g/sq cm/b.y., respectively. Chemistry below 50 km is driven mostly by photolysis of methane by the solar and interstellar medium Lyman-alpha photons, producing hydrocarbons C2H4, C2H6, and C2H2 which form haze particles with precipitation rates of 135, 28, and 1.3 g/sq cm/b.y., respectively. Some processes are discussed which increase the production of HCN (by an order of magnitude to a value of 29 g/sq cm/b.y.) and involve indirect photolysis of N2 by neutrals. Reanalysis of the measured methane profiles gives an eddy diffusion coefficient K = 4 x 10(exp 3)sq cm/s above the tropopause and a more accurate methane number density near the surface, (3.1 +/- 0.8)x IO(exp 11)/cu cm. Chemistry above 200 km is driven by the solar EUV radiation (lambda less than 1000 A) and by precipitation of magnetospheric electrons with a total energy input of 10(exp 8) W (based on thermal balance calculations). The most abundant photochemical species are N, H2, H, 0, and C. They escape with the total rates of 7.7 x 10(exp 24)/ s, 4.5 x 10(exp 25)/s, 2.4 x 10(exp 25)/s, 4.4 x 10(exp 22)/s, and 1.1 x 10(exp 24), respectively. Atomic species are transported to a region of 50-200 km and drive the chemistry there. Ionospheric chemistry explains the formation of an E region at 150-240 km with HCO(+) as a major ion, and of an F region above 240 km with a peak at 320 km and C(+) as a major ion. The ionosphere above 500 km consists of almost equal densities of C(+) and N(+) ions. The model profiles agree with the measured atomic nitrogen and electron density profiles. A number of other models with varying rate coefficients of some reactions, differing properties of the haze particles (chemically passive or active), etc., were developed. These models show that there are four basic unknown values which have strong impacts on the composition and structure of the atmosphere and ionosphere. These values and their plausible ranges are the CO mixing ratio f(sub co) = 10(exp -4) - 10(exp -3), the magnetospheric electron energy input (1 +/- 0.5) x 10(exp 8) W, the rate coefficient of charge-exchange reaction N2(+) + C(kappa) = 10(exp -11) - 10(exp -10)cu cm/s, and the ion escape velocity upsilon(sub i) approx. equals 150 cm/s.

Micellar emulsions composed of mPEG-PCL/MCT as novel nanocarriers for systemic delivery of genistein: a comparative study with micelles

PubMed Central

Zhang, Tianpeng; Wang, Huan; Ye, Yanghuan; Zhang, Xingwang; Wu, Baojian

2015-01-01

Polymeric micelles receive considerable attention as drug delivery vehicles, depending on the versatility in drug solubilization and targeting therapy. However, their use invariably suffers with poor stability both in in vitro and in vivo conditions. Here, we aimed to develop a novel nanocarrier (micellar emulsions, MEs) for a systemic delivery of genistein (Gen), a poorly soluble anticancer agent. Gen-loaded MEs (Gen-MEs) were prepared from methoxy poly(ethylene glycol)-block-(ε-caprolactone) and medium-chain triglycerides (MCT) by solvent-diffusion technique. Nanocarriers were characterized by dynamic light scattering, transmission electron microscopy, and in vitro release. The resulting Gen-MEs were approximately 46 nm in particle size with a narrow distribution. Gen-MEs produced a different in vitro release profile from the counterpart of Gen-ME. The incorporation of MCT significantly enhanced the stability of nanoparticles against dilution with simulated body fluid. Pharmacokinetic study revealed that MEs could notably extend the mean retention time of Gen, 1.57- and 7.38-fold as long as that of micelles and solution formulation, respectively, following intravenous injection. Furthermore, MEs markedly increased the elimination half-life (t1/2β) of Gen, which was 2.63-fold larger than that of Gen solution. Interestingly, Gen distribution in the liver and kidney for MEs group was significantly low relative to the micelle group in the first 2 hours, indicating less perfusion in such two tissues, which well accorded with the elongated mean retention time. Our findings suggested that MEs may be promising carriers as an alternative of micelles to systemically deliver poorly soluble drugs. PMID:26491290

Transduction of Photoreceptors With Equine Infectious Anemia Virus Lentiviral Vectors: Safety and Biodistribution of StarGen for Stargardt Disease

PubMed Central

Binley, Katie; Widdowson, Peter; Loader, Julie; Kelleher, Michelle; Iqball, Sharifah; Ferrige, Georgina; de Belin, Jackie; Carlucci, Marie; Angell-Manning, Diana; Hurst, Felicity; Ellis, Scott; Miskin, James; Fernandes, Alcides; Wong, Paul; Allikmets, Rando; Bergstrom, Christopher; Aaberg, Thomas; Yan, Jiong; Kong, Jian; Gouras, Peter; Prefontaine, Annick; Vezina, Mark; Bussieres, Martin; Naylor, Stuart; Mitrophanous, Kyriacos A.

2013-01-01

Purpose. StarGen is an equine infectious anemia virus (EIAV)-based lentiviral vector that expresses the photoreceptor-specific adenosine triphosphate (ATP)-binding cassette transporter (ABCA4) protein that is mutated in Stargardt disease (STGD1), a juvenile macular dystrophy. EIAV vectors are able to efficiently transduce rod and cone photoreceptors in addition to retinal pigment epithelium in the adult macaque and rabbit retina following subretinal delivery. The safety and biodistribution of StarGen following subretinal delivery in macaques and rabbits was assessed. Methods. Regular ophthalmic examinations, IOP measurements, ERG responses, and histopathology were carried out in both species to compare control and vector-treated eyes. Tissue and fluid samples were obtained to evaluate the persistence, biodistribution, and shedding of the vector following subretinal delivery. Results. Ophthalmic examinations revealed a slightly higher level of inflammation in StarGen compared with control treated eyes in both species. However, inflammation was transient and no overt toxicity was observed in StarGen treated eyes and there were no abnormal clinical findings. There was no StarGen-associated rise in IOP or abnormal ERG response in either rabbits or macaques. Histopathologic examination of the eyes did not reveal any detrimental changes resulting from subretinal administration of StarGen. Although antibodies to StarGen vector components were detected in rabbit but not macaque serum, this immunologic response did not result in any long-term toxicity. Biodistribution analysis demonstrated that the StarGen vector was restricted to the ocular compartment. Conclusions. In summary, these studies demonstrate StarGen to be well tolerated and localized following subretinal administration. PMID:23620430

Complete Mitochondrial Genomes of the Cherskii's Sculpin Cottus czerskii and Siberian Taimen Hucho taimen Reveal GenBank Entry Errors: Incorrect Species Identification and Recombinant Mitochondrial Genome.

PubMed

Balakirev, Evgeniy S; Saveliev, Pavel A; Ayala, Francisco J

2017-01-01

The complete mitochondrial (mt) genome is sequenced in 2 individuals of the Cherskii's sculpin Cottus czerskii . A surprisingly high level of sequence divergence (10.3%) has been detected between the 2 genomes of C czerskii studied here and the GenBank mt genome of C czerskii (KJ956027). At the same time, a surprisingly low level of divergence (1.4%) has been detected between the GenBank C czerskii (KJ956027) and the Amur sculpin Cottus szanaga (KX762049, KX762050). We argue that the observed discrepancies are due to incorrect taxonomic identification so that the GenBank accession number KJ956027 represents actually the mt genome of C szanaga erroneously identified as C czerskii . Our results are of consequence concerning the GenBank database quality, highlighting the potential negative consequences of entry errors, which once they are introduced tend to be propagated among databases and subsequent publications. We illustrate the premise with the data on recombinant mt genome of the Siberian taimen Hucho taimen (NCBI Reference Sequence Database NC_016426.1; GenBank accession number HQ897271.1), bearing 2 introgressed fragments (≈0.9 kb [kilobase]) from 2 lenok subspecies, Brachymystax lenok and Brachymystax lenok tsinlingensis , submitted to GenBank on June 12, 2011. Since the time of submission, the H taimen recombinant mt genome leading to incorrect phylogenetic inferences was propagated in multiple subsequent publications despite the fact that nonrecombinant H taimen genomes were also available (submitted to GenBank on August 2, 2014; KJ711549, KJ711550). Other examples of recombinant sequences persisting in GenBank are also considered. A GenBank Entry Error Depositary is urgently needed to monitor and avoid a progressive accumulation of wrong biological information.

GenGIS 2: Geospatial Analysis of Traditional and Genetic Biodiversity, with New Gradient Algorithms and an Extensible Plugin Framework

PubMed Central

Parks, Donovan H.; Mankowski, Timothy; Zangooei, Somayyeh; Porter, Michael S.; Armanini, David G.; Baird, Donald J.; Langille, Morgan G. I.; Beiko, Robert G.

2013-01-01

GenGIS is free and open source software designed to integrate biodiversity data with a digital map and information about geography and habitat. While originally developed with microbial community analyses and phylogeography in mind, GenGIS has been applied to a wide range of datasets. A key feature of GenGIS is the ability to test geographic axes that can correspond to routes of migration or gradients that influence community similarity. Here we introduce GenGIS version 2, which extends the linear gradient tests introduced in the first version to allow comprehensive testing of all possible linear geographic axes. GenGIS v2 also includes a new plugin framework that supports the development and use of graphically driven analysis packages: initial plugins include implementations of linear regression and the Mantel test, calculations of alpha-diversity (e.g., Shannon Index) for all samples, and geographic visualizations of dissimilarity matrices. We have also implemented a recently published method for biomonitoring reference condition analysis (RCA), which compares observed species richness and diversity to predicted values to determine whether a given site has been impacted. The newest version of GenGIS supports vector data in addition to raster files. We demonstrate the new features of GenGIS by performing a full gradient analysis of an Australian kangaroo apple data set, by using plugins and embedded statistical commands to analyze human microbiome sample data, and by applying RCA to a set of samples from Atlantic Canada. GenGIS release versions, tutorials and documentation are freely available at http://kiwi.cs.dal.ca/GenGIS, and source code is available at https://github.com/beiko-lab/gengis. PMID:23922841

Constraining Dark Matter Models from a Combined Analysis of Milky Way Satellites with the Fermi Large Area Telescope

NASA Technical Reports Server (NTRS)

Ackermann, M.; Ajello, M.; Albert, A.; Atwood, W. B.; Baldini, L.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Bechtol, K.; Bellazzini, R.;

Limited capacity for contour curvature in iconic memory.

PubMed

Sakai, Koji

2006-06-01

We measured the difference threshold for contour curvature in iconic memory by using the cued discrimination method. The study stimulus consisting of 2 to 6 curved contours was briefly presented in the fovea, followed by two lines as cues. Subjects discriminated the curvature of two cued curves. The cue delays were 0 msec. and 300 msec. in Exps. 1 and 2, respectively, and 50 msec. before the study offset in Exp. 3. Analysis of data from Exps. 1 and 2 showed that the Weber fraction rose monotonically with the increase in set size. Clear set-size effects indicate that iconic memory has a limited capacity. Moreover, clear set-size effect in Exp. 3 indicates that perception itself has a limited capacity. Larger set-size effects in Exp. 1 than in Exp. 3 suggest that iconic memory after perceptual process has limited capacity. These properties of iconic memory at threshold level are contradictory to the traditional view that iconic memory has a high capacity both at suprathreshold and categorical levels.

Crystal Growth and Characterization of CdTe Grown by Vertical Gradient Freeze

NASA Technical Reports Server (NTRS)

Su, Ching-Hua; Lehoczky, S. L.; Raghothamachar, B.; Dudley, M.

2007-01-01

In this study, crystals of CdTe were grown from melts by the unseeded vertical gradient freeze method. The quality of grown crystal were studied by various characterization techniques including Synchrotron White Beam X-ray Topography (SWBXT), chemical analysis by glow discharge mass spectroscopy (GDMS), low temperature photoluminescence (PL), and Hall measurements. The SWBXT images from various angles show nearly strain-free grains, grains with inhomogeneous strains, as well as twinning nucleated in the shoulder region of the boule. The GDMS chemical analysis shows the contamination of Ga at a level of 3900 ppb, atomic. The low temperature PL measurement exhibits the characteristic emissions of a Ga-doped sample. The Hall measurements show a resistivity of 1 x l0(exp 7) ohm-cm at room temperature to 3 x 10(exp 9) ohm-cm at 78K with the respective hole and electron concentration of 1.7 x 10(exp 9) cm(exp -3) and 3.9 x 10(exp 7) cm(exp -3) at room temperature.

The microgravity environment of the Space Shuttle Columbia middeck during STS-32

NASA Technical Reports Server (NTRS)

Dunbar, Bonnie J.; Thomas, Donald A.; Schoess, Jeff N.

1991-01-01

Four hours of three-axis microgravity accelerometer data were successfully measured at the MA9F locker location in the Orbiter middeck of Columbia as part of the Microgravity Disturbances Experiment (MDE) on STS-32. These data were measured using the Honeywell In-Space Accelerometer, a small three-axis accelerometer that was hard-mounted onto the Fluid Experiment Apparatus to record the microgravity environment at the exact location of the MDE. Data were recorded during specific mission events such as Orbiter quiescent periods, crew exercise on the treadmill, and numerous Orbiter engine burns. Orbiter background levels were measured to be in the 3 x 10(exp -5) to 2 x 10(exp -4) G range, treadmill operations in the 6 x 10(exp -4) to 5 x 10(exp -3) G range, and Orbiter engine burns from 4 x 10(exp -3) to in excess of 1 x 10(exp -2) G. These data represent some of the first microgravity accelerometer data ever recorded in the middeck area of the Orbiter.

Scalability Test of Multiscale Fluid-Platelet Model for Three Top Supercomputers

PubMed Central

Zhang, Peng; Zhang, Na; Gao, Chao; Zhang, Li; Gao, Yuxiang; Deng, Yuefan; Bluestein, Danny

2016-01-01

We have tested the scalability of three supercomputers: the Tianhe-2, Stampede and CS-Storm with multiscale fluid-platelet simulations, in which a highly-resolved and efficient numerical model for nanoscale biophysics of platelets in microscale viscous biofluids is considered. Three experiments involving varying problem sizes were performed: Exp-S: 680,718-particle single-platelet; Exp-M: 2,722,872-particle 4-platelet; and Exp-L: 10,891,488-particle 16-platelet. Our implementations of multiple time-stepping (MTS) algorithm improved the performance of single time-stepping (STS) in all experiments. Using MTS, our model achieved the following simulation rates: 12.5, 25.0, 35.5 μs/day for Exp-S and 9.09, 6.25, 14.29 μs/day for Exp-M on Tianhe-2, CS-Storm 16-K80 and Stampede K20. The best rate for Exp-L was 6.25 μs/day for Stampede. Utilizing current advanced HPC resources, the simulation rates achieved by our algorithms bring within reach performing complex multiscale simulations for solving vexing problems at the interface of biology and engineering, such as thrombosis in blood flow which combines millisecond-scale hematology with microscale blood flow at resolutions of micro-to-nanoscale cellular components of platelets. This study of testing the performance characteristics of supercomputers with advanced computational algorithms that offer optimal trade-off to achieve enhanced computational performance serves to demonstrate that such simulations are feasible with currently available HPC resources. PMID:27570250

Hand-held optical imager (Gen-2): improved instrumentation and target detectability

PubMed Central

Gonzalez, Jean; DeCerce, Joseph; Erickson, Sarah J.; Martinez, Sergio L.; Nunez, Annie; Roman, Manuela; Traub, Barbara; Flores, Cecilia A.; Roberts, Seigbeh M.; Hernandez, Estrella; Aguirre, Wenceslao; Kiszonas, Richard

2012-01-01

Abstract. Hand-held optical imagers are developed by various researchers towards reflectance-based spectroscopic imaging of breast cancer. Recently, a Gen-1 handheld optical imager was developed with capabilities to perform two-dimensional (2-D) spectroscopic as well as three-dimensional (3-D) tomographic imaging studies. However, the imager was bulky with poor surface contact (∼30%) along curved tissues, and limited sensitivity to detect targets consistently. Herein, a Gen-2 hand-held optical imager that overcame the above limitations of the Gen-1 imager has been developed and the instrumentation described. The Gen-2 hand-held imager is less bulky, portable, and has improved surface contact (∼86%) on curved tissues. Additionally, the forked probe head design is capable of simultaneous bilateral reflectance imaging of both breast tissues, and also transillumination imaging of a single breast tissue. Experimental studies were performed on tissue phantoms to demonstrate the improved sensitivity in detecting targets using the Gen-2 imager. The improved instrumentation of the Gen-2 imager allowed detection of targets independent of their location with respect to the illumination points, unlike in Gen-1 imager. The developed imager has potential for future clinical breast imaging with enhanced sensitivity, via both reflectance and transillumination imaging. PMID:23224163

NextGen Home Sperm Banking Kit: Outcomes of Offsite vs Onsite Collection--Preliminary Findings.

PubMed

Agarwal, Ashok; Sharma, Reecha; Gupta, Sajal; Sharma, Rakesh

2015-06-01

To compare cryosurvival rates between remote collections with NextGen kit (offsite) and onsite collection of semen samples from infertile men and those with cancer. Prefreeze and post-thaw sperm motility, total motile sperm, and percent cryosurvival rates were compared between samples collected from infertile men onsite at the Andrology Center (n = 10) and samples collected from infertile patients at home (offsite; n = 9), which were shipped by NextGen to our laboratory. A second group (n = 17) consisted of 10 semen samples from cancer patients collected onsite, which were compared with 7 semen samples from cancer patients shipped by the NextGen. All semen samples were assessed within 18 hours of collection. In the infertile men, percent cryosurvival rates were similar with NextGen compared with those of onsite collection (53.14 ± 28.9% vs 61.90 ± 20.46%; P = .51). Similarly, in the cancer patients, all 4 parameters were comparable between the onsite and NextGen. Cryosurvival rates were also similar between NextGen compared with those of onsite collection (52.71 ± 20.37% vs 58.90 ± 22.68%; P = .46). Cancer patients can bank sperm as effectively as men banking for infertility reasons using the NextGen kit. Copyright © 2015 Elsevier Inc. All rights reserved.

Measurement and Control of Oxygen Partial Pressure in an Electrostatic Levitator

NASA Technical Reports Server (NTRS)

SanSoucie, Michael P.; Rogers, Jan R.

2014-01-01

Recently the NASA Marshall Space Flight Center electrostatic levitation (ESL) laboratory has been upgraded to include an oxygen control system. This system allows the oxygen partial pressure within the vacuum chamber to be measured and controlled, at elevated temperatures, theoretically in the range from 10(exp -36) to 10(exp 0) bar. The role of active surface agents in liquid metals is fairly well known; however, published surface tension data typically has large scatter, which has been hypothesized to be caused by the presence of oxygen. The surface tension of metals is affected by even a small amount of adsorption of oxygen. It has even been shown that oxygen partial pressures may need to be as low as 10(exp -24) bar to avoid oxidation. While electrostatic levitation is done under high vacuum, oxide films or dissolved oxygen may have significant effects on materials properties, such as surface tension and viscosity. Therefore, the ability to measure and control the oxygen partial pressure within the chamber is highly desirable. The oxygen control system installed at MSFC contains a potentiometric sensor, which measures the oxygen partial pressure, and an oxygen ion pump. In the pump, a pulse-width modulated electric current is applied to yttrium-stabilized zirconia, resulting in oxygen transfer into or out of the system. Also part of the system is a control unit, which consists of temperature controllers for the sensor and pump, PID-based current loop for the ion pump, and a control algorithm. This system can be used to study the effects of oxygen on the thermophysical properties of metals, ceramics, glasses, and alloys. It can also be used to provide more accurate measurements by processing the samples at very low oxygen partial pressures. The oxygen control system will be explained in more detail and an overview of its use and limitations in an electrostatic levitator will be described. Some preliminary measurements have been made, and the results to date will be provided.

A Spectacular Radio Flare from XRF 050416a at 40 Days and Implications for the Nature of X-Ray Flashes

NASA Technical Reports Server (NTRS)

Soderberg, A. M.; Nakar, E.; Cenko, S. B.; Cameron, P. B.; Frail, D. A.; Kulkarni, S. R.; Fox, D. B.; Berger, E.; Gal-Yam, A.; Moon, D-S.;

Friction and Wear Properties of As-deposited and Carbon Ion-implanted Diamond Films

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa

1994-01-01

Recent work on the friction and wear properties of as-deposited and carbon ion-implanted diamond films was reviewed. Diamond films were produced by the microwave plasma chemical vapor deposition (CVD) technique. Diamond films with various grain sizes and surface roughnesses were implanted with carbon ions at 60 ke V ion energy, resulting in a dose of 1.2310(exp 17) carbon ions/cm(exp 2). Various analytical techniques, including Raman spectroscopy, proton recoil analysis, Rutherford backscattering, transmission and scanning electron microscopy, x-ray photoelectron spectroscopy, and x-ray diffraction, were utilized to characterize the diamond films. Sliding friction experiments were conducted with a polished natural diamond pin in contact with diamond films in the three environments: humid air (40 percent relative humidity), dry nitrogen (less than 1 percent relative humidity), and ultrahigh vacuum (10(exp -7) Pa). The CVD diamond films indeed have friction and were properties similar to those of natural diamond in the three environments. The as-deposited, fine-grain diamond films can be effectively used as self-lubricating, wear-resistant coatings that have low coefficients of friction (0.02 to 0.04) and low wear rates (10(exp -7) to 10(exp -8)mm(exp 3)/N-m) in both humid air and dry nitrogen. However, they have high coefficients of friction (1.5 to 1.7) and a high wear rate (10(exp -4)mm(exp 3/N-m) in ultrahigh vacuum. The carbon ion implanation produced a thin surficial layer (less than 0.1 micron thick) of amorphous, nondiamond carbon on the diamond films. In humid air and dry nitrogen, the ion-implanted, fine- and coarse-grain diamond films have a low coefficient of friction (around 0.1) and a low wear rate (10(exp -7)mm(exp 3/N-m). Even in ultrahigh vacuum, the presence of the nondiamond carbon layer reduced the coefficient of friction of fine-grain diamond films to 0.1 or lower and the wear rate to 10(exp -6)mm(exp 3)/N-m. Thus, the carbon ion-implanted, fine-grain diamond films can be effectively used as wear-resistant, self-lubricating coatings not only in air and dry nitrogen, but also in ultrahigh vacuum. The wear mechanism of diamond films is that of small fragments chipping off the surface. The size of wear particles is related to the extent of wear rates.

Characteristics of the Dust-Plasma Interaction Near Enceladus' South Pole

NASA Technical Reports Server (NTRS)

Shafiq, Muhammad; Wahlund, J.-E.; Morooka, M. W; Kurth, W. S.; Farrell, W. M.

2010-01-01

We present RPWS Langmuir probe data from the third Enceladus flyby (E3) showing (he presence of dusty plasma near Enceladus' South Pole. There is a sharp rise in both the electron and ion number densities when the spacecraft traverses through Enceladus plume. The ion density near Enceladus is found to increase abruptly from about 10(exp 2) cm (exp -3) before the closest approach to 10(exp 5) cm (exp -3) just 30 s after the closest approach, an amount two orders of magnitude higher than the electron density. Assuming that the inconsistency between the electron and ion number densities is due to the presence of dust particles that are collecting the missing electron charges, we present dusty plasma characteristics down to sub-micron particle sizes. By assuming a differential dust number density for a range in dust sizes and by making use of Langmuir probe data, the dust densities for certain lower limits in dust size distribution were estimated. In order to achieve the dust densities of micrometer and larger sized grains comparable to the ones reported in the literature. we show that the power law size distribution must hold down to at least 0.03 micron such that the total differential number density is dominated by the smallest sub-micron sized grains. The total dust number density in Enceladus' plume is of the order of l0(exp 2) cm(exp -3) reducing to 1 cm(exp -3) in the E- ring. The dust density for micrometer and larger sized grains is estimated to be about 10(exp -4) cm(exp -3) in the plume while it is about 10(exp -6) - 10(exp -7) cm(exp -3) in the E-ring. Dust charge for micron sized grains is estimated to be about eight thousand electron charges reducing to below one hundred electron charges for 0.03 micron sized grains. The effective dusty plasma Debye length is estimated and compared with intergrain distance as well as the electron Debye length. The maximum dust charging time of 1.4 h is found for 0.03 11mmicron sized grains just 1 min before the closest approach. The charging time decreases substantially in the plume where it is only a fraction of a second for 1 micron sized grains, 1 s for 0.l micron sized grains and about 10 s for 0.03 micron sized grains.

GenBank.

PubMed

Benson, Dennis A; Karsch-Mizrachi, Ilene; Lipman, David J; Ostell, James; Sayers, Eric W

2010-01-01

GenBank is a comprehensive database that contains publicly available nucleotide sequences for more than 300,000 organisms named at the genus level or lower, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole genome shotgun (WGS) and environmental sampling projects. Most submissions are made using the web-based BankIt or standalone Sequin programs, and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the European Molecular Biology Laboratory Nucleotide Sequence Database in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through the NCBI Entrez retrieval system, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bi-monthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI homepage: www.ncbi.nlm.nih.gov.

Using GenBank.

PubMed

Wheeler, David

2007-01-01

GenBank(R) is a comprehensive database of publicly available DNA sequences for more than 205,000 named organisms and for more than 60,000 within the embryophyta, obtained through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Daily data exchange with the European Molecular Biology Laboratory (EMBL) in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through the National Center for Biotechnology Information (NCBI) retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases with taxonomy, genome, mapping, protein structure, and domain information and the biomedical journal literature through PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available through FTP. GenBank usage scenarios ranging from local analyses of the data available through FTP to online analyses supported by the NCBI Web-based tools are discussed. To access GenBank and its related retrieval and analysis services, go to the NCBI Homepage at http://www.ncbi.nlm.nih.gov.

GenBank.

PubMed

Benson, Dennis A; Karsch-Mizrachi, Ilene; Lipman, David J; Ostell, James; Sayers, Eric W

2011-01-01

GenBank® is a comprehensive database that contains publicly available nucleotide sequences for more than 380,000 organisms named at the genus level or lower, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects, including whole genome shotgun (WGS) and environmental sampling projects. Most submissions are made using the web-based BankIt or standalone Sequin programs, and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the European Nucleotide Archive (ENA) and the DNA Data Bank of Japan (DDBJ) ensures worldwide coverage. GenBank is accessible through the NCBI Entrez retrieval system that integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI Homepage: www.ncbi.nlm.nih.gov.

GenBank.

PubMed

Benson, Dennis A; Karsch-Mizrachi, Ilene; Lipman, David J; Ostell, James; Sayers, Eric W

2009-01-01

GenBank is a comprehensive database that contains publicly available nucleotide sequences for more than 300,000 organisms named at the genus level or lower, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or standalone Sequin programs, and accession numbers are assigned by GenBank(R) staff upon receipt. Daily data exchange with the European Molecular Biology Laboratory Nucleotide Sequence Database in Europe and the DNA Data Bank of Japan ensures worldwide coverage. GenBank is accessible through the National Center for Biotechnology Information (NCBI) Entrez retrieval system, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, begin at the NCBI Homepage: www.ncbi.nlm.nih.gov.

Optimized Gen-II FeCrAl cladding production in large quantity for campaign testing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yamamoto, Yukinori; Sun, Zhiqian; Pint, Bruce A.

2016-06-03

There are two major objectives in this report; (1) to optimize microstructure control of ATF FeCrAl alloys during tube drawing processes, and (2) to provide an update on the progress of ATF FeCrAl tube production via commercial manufacturers. Experimental efforts have been made to optimize the process parameters balancing the tube fabricability, especially for tube drawing processes, and microstructure control of the final tube products. Lab-scale sheet materials of Gen II FeCrAl alloys (Mo-containing and Nb-containing FeCrAl alloys) were used in the study, combined with a stepwise warm-rolling process and intermediate annealing, aiming to simulate the tube drawing process inmore » a commercial tube manufacturer. The intermediate annealing at 650ºC for 1h was suggested for the tube-drawing process of Mo-containing FeCrAl alloys because it successfully softened the material by recovering the work hardening introduced through the rolling step, without inducing grain coarsening due to recrystallization. The final tube product is expected to have stabilized deformed microstructure providing the improved tensile properties with sufficient ductility. Optimization efforts on Nb-containing FeCrAl alloys focused on the effect of alloying additions and annealing conditions on the stability of deformed microstructure. Relationships between the second-phase precipitates (Fe 2Nb-Laves phase) and microstructure stability are discussed. FeCrAl tube production through commercial tube manufacturers is currently in progress. Three different manufacturers, Century Tubes, Inc. (CTI), Rhenium Alloys, Inc. (RAI), and Superior Tube Company, Inc. (STC), are providing capabilities for cold-drawing, warm-drawing, and HPTR cold-pilgering, respectively. The first two companies are currently working on large quantity tube production (expected 250 ft length) of Gen I model FeCrAl alloy (B136Y3, at CTI) and Gen II (C35M4, at RAI), with the process parameters obtained from the experimental efforts. The expected delivery dates are at the end of July, 2016, and the middle of June, 2016, respectively. Tube production at STC would be the first attempt to apply cold-pilgering to the FeCrAl alloys. Communication has been initiated, and the materials have been machined for the cold-pilgering process.« less

Satellite measurements of whistler dispersion at low latitudes

NASA Astrophysics Data System (ADS)

Hughes, A. R. W.

Dispersion measurements for whistlers recorded on ISIS 2 passes over Quito, Equador show that the most numerous whistlers were those with dispersions which increased from about 5 sec (exp 1/2) at 30 deg magnetic latitude to between 10 and 12 sec (exp 1/2) at the magnetic equator. Less numerous whistlers had dispersions which decreased from 25 sec (exp 1/2) at 30 deg to between 10 and 12 sec (exp 1/2) at the equator. The measured dispersions show the measured whistlers to have been unducted.

Disruption of Calcium Homeostasis during Exercise as a Mediator of Bone Metabolism

DTIC Science & Technology

2014-10-01

2013. We met the goal of having 14 women and 14 men complete EXP1. Progress on EXP1: Enrolled Screen Withdrew Randomized Withdrew Completed Failure...Before After Randomized Randomized Women 18 2 1 15 1 14 Men 22 2 3 17 3 14 Total 40 4 4 32 4 28 Reasons for withdrawals: Screening failures...Prepare annual progress report in Q4 This was accomplished. • Data from EXP1 As planned, 14 women and 14 men completed EXP1. The characteristics of

Rosat Observations of Nine Globular Clusters

NASA Technical Reports Server (NTRS)

Rappaport, S.; Dewey, D.; Levine, A.; Macri, L.

1994-01-01

The ROSAT HRI was used to image fields around nine Galactic globular clusters that have central densities in the range of 10(exp 4) - 10(exp 5) solar mass pc(exp -3) and that had not previously been observed with the Einstein Observatory. We detected X-ray sources associated with Pal 2 and NGC 6304 with luminosities of 1.1 x 10(exp 34) ergs/s and 1.2 x 10(exp 33) ergs/s, respectively. No X-ray emission was detected from the source in Ter 6, thus confirming its transient nature. In all, there were 23 serendipitous sources found in the nine fields; none was apparently associated with any of the other seven clusters. The results are discussed in the context of low-luminosity cluster X-ray sources, in general.

First images of a possible CO(+)-tail of comet P/Schwassmann-Wachmann 1 observed against the dust coma background

NASA Technical Reports Server (NTRS)

Jockers, Klaus; Bonev, T.; Ivanova, Violeta; Rauer, H.

1992-01-01

Comet P/Schwassmann-Wachmann 1 was observed with the 2m-Ritchey-Cretien Telescope of the Bulgarian National Observatory, Rozhen, Bulgaria, using the CCD-camera and focal reducer of the Max-Planck-Institute for Aeronomy. Images were taken in a red continuum window and in the 2-0 A(exp 2)Pi - X(exp 2)Sigma(+) band of CO(+) located in the blue part of the spectrum. The red images reveal an extended dust coma. From a comparison of the red and blue images a dust reddening of 13.2 percent per 1000 A is derived. At 642 nm the magnitude of the comet with a square diaphragm of 4.5 arcsec is 16.6. The blue images, taken in the CO(+) band, show a significantly different brightness distribution which is interpreted as presence of a CO(+) coma and tail superimposed on the continuum. A column density of several 10(exp 10) CO(+) molecules cm(exp -2) is derived. The tail thickness of 10(exp 5) km is unexpectedly small. We estimate the CO(+) production rate to about 6 x 10(exp 26) CO(+) particles s(exp -1). This value does not support the idea that the outbursts of this comet are caused by crystallization of amorphous water ice.

Predictors of the Perception of Smoking Health Risks in Smokers With or Without Schizophrenia.

PubMed

Kowalczyk, William J; Wehring, Heidi J; Burton, George; Raley, Heather; Feldman, Stephanie; Heishman, Stephen J; Kelly, Deanna L

2017-01-01

This study sought to examine the predictors of health risk perception in smokers with or without schizophrenia. The health risk subscale from the Smoking Consequences Questionnaire was dichotomized and used to measure health risk perception in smokers with (n = 67) and without schizophrenia (n = 100). A backward stepwise logistic regression was conducted using variables associated at the bivariate level to determine multivariate predictors. Overall, 62.5% of smokers without schizophrenia and 40.3% of smokers with schizophrenia completely recognize the health risks of smoking (p ≤ .01). Multivariate predictors for smokers without schizophrenia included: sex (Exp (B) = .3; p < .05), Smoking Consequences Questionnaire state enhancement (Exp (B) = .69; p < .01), and craving relief (Exp (B) = 1.8; p < .01). Among smokers with schizophrenia, predictors were education (Exp (B) = .7; p < .05), nicotine dependence (Exp (B) = .5; p < .01), motivation to quit (Exp (B) = 1.8; p < .01), and Smoking Consequences Questionnaire craving relief (Exp (B) = 1.8; p < .01). There was overlap and differences between predictors in smokers with and without schizophrenia. Commonly used techniques for education on the health consequences of cigarettes may work in smokers with schizophrenia, but intervention efforts specifically tailored to smokers with schizophrenia might be more efficacious.

Predictors of the Perception of Smoking Health Risks in Smokers With or Without Schizophrenia

PubMed Central

Kowalczyk, William J.; Wehring, Heidi J.; Burton, George; Raley, Heather; Feldman, Stephanie; Heishman, Stephen J.; Kelly, Deanna L.

2017-01-01

Objective This study sought to examine the predictors of health risk perception in smokers with or without schizophrenia. Methods The health risk subscale from the Smoking Consequences Questionnaire was dichotomized and used to measure health risk perception in smokers with (n = 67) and without schizophrenia (n = 100). A backward stepwise logistic regression was conducted using variables associated at the bivariate level to determine multivariate predictors. Results Overall, 62.5% of smokers without schizophrenia and 40.3% of smokers with schizophrenia completely recognize the health risks of smoking (p ≤ .01). Multivariate predictors for smokers without schizophrenia included: sex (Exp (B) = .3; p < .05), Smoking Consequences Questionnaire state enhancement (Exp (B) = .69; p < .01), and craving relief (Exp (B) = 1.8; p < .01). Among smokers with schizophrenia, predictors were education (Exp (B) = .7; p < .05), nicotine dependence (Exp (B) = .5; p < .01), motivation to quit (Exp (B) = 1.8; p < .01), and Smoking Consequences Questionnaire craving relief (Exp (B) = 1.8; p < .01). Conclusions There was overlap and differences between predictors in smokers with and without schizophrenia. Commonly used techniques for education on the health consequences of cigarettes may work in smokers with schizophrenia, but intervention efforts specifically tailored to smokers with schizophrenia might be more efficacious. PMID:27858591

Baseline differences and trajectories of change for deceased, placed, and community residing Alzheimer disease patients.

PubMed

Schulz, Richard; Zdaniuk, Bozena; Belle, Steven H; Czaja, Sara J; Michael Arrighi, Henry; Zbrozek, Arthur S

2010-01-01

This study identifies predictors of placement or death in a large ethnically/racially diverse sample of moderately impaired Alzheimer disease patients residing in the community. Patients and caregivers were followed for 18 months with 4 assessments at 6-month intervals. Multinomial regression was used to identify caregiver and patient baseline characteristics and changes over time as predictors of patient placement in a long-term care facility (n=180), patient death (not preceded by placement, n=187), or remaining in the community at home (n=583). Our findings reveal important differences between death and placement when compared with continued home care. Both death and placement are significantly associated with increased activities of daily living limitations (Exp(B)=1.285, P=0.017; Exp(B)=0.1.202, P=0.038, for death and placement, compared with home care, respectively), having a nonspouse caregiver [Exp(B)=0.325, P=0.026; Exp(B)=0.386, P=0.050, for death and placement, respectively], and being a male patient [Exp(B)=0.367, P=0.003; Exp(B)=0.439, P=0.016, for death and placement, respectively]. Death and placement differ with respect to health service use, race, and group assignment. Whites are more likely to be placed rather than remain at home when compared with African American [Ex(B)=0.520, P=0.028] or Hispanic [Exp(B)=0.338, P<0.005] patients, whereas being assigned to the control condition as opposed to active treatment [Exp(B)=0.515, P=0.008], having a male caregiver [Exp(B)=0.482, P=0.043], and increasing patient health service use [Exp(B)=1.105, P=0.015] are associated with increased mortality. Placed and deceased patients are further differentiated from each other by the fact that caregivers of placed patients report an increase in being bothered by memory problems when compared with caregivers of deceased patients [Exp(B)=0.577, P=0.006]. Patients who are placed, died, or remain at home have unique trajectories, which vary as a function of the reference group used for comparison. Increasing bother with memory problems is uniquely associated with placement relative to death while increasing health service use in the form of physician contacts and nurses visits is uniquely associated with death among community residing Alzheimer disease patients.

Reproductive and growth responses of gilts to exogenous porcine pituitary growth hormone.

PubMed

Bryan, K A; Hammond, J M; Canning, S; Mondschein, J; Carbaugh, D E; Clark, A M; Hagen, D R

1989-01-01

Forty gilts (mean wt = 72 kg) were administered daily either vehicle (C = control) or 70 micrograms porcine growth hormone (pGH)/kg BW. After 30 d of treatment, eight gilts per group (Exp. 1) were slaughtered and blood, uteri and ovaries were collected. Follicular fluid (FFl) was collected and granulosa cells (GC) were cultured. The remaining gilts (Exp. 2) were treated for up to 35 additional days and examined twice daily for estrus. Estrusal gilts were removed from the experiment. Noncyclic gilts (n = 9 of 12 pGH; n = 4 of 12 C) were slaughtered on d 66 and their ovaries were examined. Ovarian weights were not different for pGH and C gilts in either Exp. 1 (P greater than .1) or Exp. 2 (P = .09). Uterine weights were greater for pGH-treated than for C gilts (P less than .007) in Exp. 1, but not in Exp. 2. Concentrations of estradiol (E2) in plasma and FF1 and of progesterone (P) in plasma and FF1 were not different for pGH and C gilts. Concentrations of insulin-like growth factor-I (IGF-I) in FF1 and in serum were greater for pGH than for C gilts (P less than .01). Concentration of P in serum-free medium of cultured GC was lower for GH than for C (P less than .05) in the presence or absence of gonadotropins in Exp. 1. The FSH-stimulated secretion of P was also lower for GC of pGH-treated gilts in Exp. 2, indicating a failure of GC to differentiate in culture. Only one pGH gilts in Exp. 2 manifested estrus, compared with seven C gilts (P less than .025). In Exp. 1, ADG was higher (P less than .03) and feed/gain lower (P less than .07) for pGH gilts. Longissimus muscle area (LMA) was not different (P = .19) between groups. Backfat thickness (BF) was lower (P less than .005) in pGH than in C in both Exp. 1 and 2. We conclude that exogenous pGH increased growth rate, improved feed efficiency and altered carcass traits in gilts. However, these effects were associated with impaired ovarian development of prepubertal gilts and a low incidence of estrus.

Moth flies and sand flies (Diptera: Psychodidae) in Cretaceous Burmese amber

PubMed Central

Solórzano Kraemer, Mónica M.; Ibáñez-Bernal, Sergio; Wagner, Rüdiger

2015-01-01

One new subfamily, four new genera and 10 new species of Psychodidae are described from Burmese amber which significantly increases our knowledge about this group in the Cretaceous. Protopsychodinae n. subfam. probably represents the oldest known ancestor of modern Psychodinae and includes three species within two genera: Datzia setosa gen. et sp. n., Datzia bispina gen. et sp. n., and Mandalayia beumersorum gen. et sp. n. Sycoracinae and Phlebotominae are represented by two genera each in the studied material, Palaeoparasycorax globosus gen. et sp. n., Palaeoparasycorax suppus gen. et sp. n., Parasycorax simplex sp. n., and Phlebotomites aphoe sp. n. and Phlebotomus vetus sp. n., respectively. Bruchomyiinae is represented by Nemopalpus quadrispiculatus sp. n. Furthermore, one genus of an incertae sedis subfamily, Bamara groehni gen. et sp. n., is described. The systematic positions of the new taxa are discussed. PMID:26401462

Neonatal exposure to daidzein, genistein, or the combination modulates bone development in female CD-1 mice.

PubMed

Kaludjerovic, Jovana; Ward, Wendy E

2009-03-01

Neonatal exposure to genistein (GEN), an isoflavone abundant in soy, favorably modulates bone mineral density (BMD) and bone strength in mice at adulthood. The study objective was to determine whether early exposure to a combination of the soy isoflavones daidzein (DAI) and GEN that naturally exists in soy protein-based infant formula results in greater benefits to bone at adulthood than either treatment alone. Male and female CD-1 mice (n = 8-16 pups per group per gender) were randomized to subcutaneous injections of DAI (2 mg x kg body weight(-1) x d(-1)), GEN (5 mg x kg body weight(-1) x d(-1)), DAI+GEN (7 mg x kg body weight(-1) x d(-1)), diethylstilbesterol (DES; positive control) (2 mg x kg body weight(-1) x d(-1)), or control (CON) from postnatal d 1-5 and were studied to 4 mo of age. BMD, biomechanical bone strength, and bone microarchitecture were assessed at the femur and lumbar vertebrae (LV). Females treated with DAI, GEN, DAI+GEN, or DES had greater (P < 0.05) BMD at the LV compared with CON and vertebra in the DAI and DES group were more resistant to compression fractures. Microstructural analyses demonstrated that treatment with DAI and GEN resulted in greater (P < 0.05) trabecular connectivity and trabecular thickness, respectively, than the CON. In conclusion, neonatal exposure to DAI and/or GEN had a positive effect on the skeleton of female mice at adulthood, but, compared with individual treatments, DAI+GEN did not have a greater benefit to bone in females or males.

AutoGen Version 5.0

NASA Technical Reports Server (NTRS)

Gladden, Roy E.; Khanampornpan, Teerapat; Fisher, Forest W.

2010-01-01

Version 5.0 of the AutoGen software has been released. Previous versions, variously denoted Autogen and autogen, were reported in two articles: Automated Sequence Generation Process and Software (NPO-30746), Software Tech Briefs (Special Supplement to NASA Tech Briefs), September 2007, page 30, and Autogen Version 2.0 (NPO- 41501), NASA Tech Briefs, Vol. 31, No. 10 (October 2007), page 58. To recapitulate: AutoGen (now signifying automatic sequence generation ) automates the generation of sequences of commands in a standard format for uplink to spacecraft. AutoGen requires fewer workers than are needed for older manual sequence-generation processes, and greatly reduces sequence-generation times. The sequences are embodied in spacecraft activity sequence files (SASFs). AutoGen automates generation of SASFs by use of another previously reported program called APGEN. AutoGen encodes knowledge of different mission phases and of how the resultant commands must differ among the phases. AutoGen also provides means for customizing sequences through use of configuration files. The approach followed in developing AutoGen has involved encoding the behaviors of a system into a model and encoding algorithms for context-sensitive customizations of the modeled behaviors. This version of AutoGen addressed the MRO (Mars Reconnaissance Orbiter) primary science phase (PSP) mission phase. On previous Mars missions this phase has more commonly been referred to as mapping phase. This version addressed the unique aspects of sequencing orbital operations and specifically the mission specific adaptation of orbital operations for MRO. This version also includes capabilities for MRO s role in Mars relay support for UHF relay communications with the MER rovers and the Phoenix lander.

SAGE II observations of a previously unreported stratospheric volcanic aerosol cloud in the northern polar summer of 1990

NASA Technical Reports Server (NTRS)

Yue, Glenn K.; Veiga, Robert E.; Wang, Pi-Huan

1994-01-01

Analysis of aerosol extinction profiles obtained by the spaceborne SAGE II sensor reveals that there was an anomalous increase of aerosol extinction below 18.5 km at latitudes poleward of 50 deg N from July 28 to September 9, 1990. This widespread increase of aerosol extinction in the lower stratosphere was apparently due to a remote high-latitude volcanic eruption that has not been reported to date. The increase in stratospheric optical depth in the northern polar region was about 50% in August and had diminished by October 1990. This eruption caused an increase in stratospheric aerosol mass of about 0.33 x 10(exp 5) tons, assuming the aerosol was composed of sulfuric acid and water.

Preliminary analyses of WL experiment No. 701, space environment effects on operating fiber optic systems

NASA Technical Reports Server (NTRS)

Taylor, E. W.; Berry, J. N.; Sanchez, A. D.; Padden, R. J.; Chapman, S. P.

1992-01-01

A brief overview of the analyses performed to date on WL Experiment-701 is presented. Four active digital fiber optic links were directly exposed to the space environment for a period of 2114 days. The links were situated aboard the Long Duration Exposure Facility (LDEF) with the cabled, single fiber windings atop an experimental tray containing instrumentation for exercising the experiment in orbit. Despite the unplanned and prolonged exposure to trapped and galactic radiation, wide temperature extremes, atomic oxygen interactions, and micro-meteorite and debris impacts, in most instances the optical data links performed well within the experimental limits. Analysis of the recorded orbital data clearly indicates that fiber optic applications in space will meet with success. Ongoing tests and analysis of the experiment at the Phillips Laboratory's Optoelectronics Laboratory will expand this premise, and establish the first known and extensive database of active fiber optic link performance during prolonged space exposure. WL Exp-701 was designed as a feasibility demonstration for fiber optic technology in space applications, and to study the performance of operating fiber systems exposed to space environmental factors such as galactic radiation, and wide temperature cycling. WL Exp-701 is widely acknowledged as a benchmark accomplishment that clearly demonstrates, for the first time, that fiber optic technology can be successfully used in a variety of space applications.

The Morphology of Passively Evolving Galaxies at Z-2 from HST/WFC3 in the Hubble Ultra Deep Field

NASA Technical Reports Server (NTRS)

Cassata, P.; Giavalisco, M.; Guo, Yicheng; Ferguson, H.; Koekemoer, A.; Renzini, A.; Fontana, A.; Salimbeni, S.; Dickinson, M.; Casertano, S.;

Dietary genistein supplementation in laying broiler breeder hens alters the development and metabolism of offspring embryos as revealed by hepatic transcriptome analysis.

PubMed

Lv, Zengpeng; Fan, Hao; Zhang, Beibei; Ning, Chao; Xing, Kun; Guo, Yuming

2018-03-08

Genistein (GEN) is a type of isoflavone mainly derived from soy products. In this experiment, we added 40 and 400 mg/kg GEN to the diet of laying broiler breeder hens to clarify the maternal effects of GEN on the development and metabolism of chick embryos. GEN treatment at 40 mg/kg increased embryonic length, weight, and liver index, as well as the width of the proliferative zone in the tibial growth plate of chick embryos. Gene ontology (GO) cluster analysis of the hepatic transcriptome showed that GEN treatment promoted embryonic development and cell proliferation. Low-dose GEN treatment increased insulin growth factor-binding protein (IGFBP)3 mRNA expression in the embryonic liver, whereas high-dose GEN treatment increased IGFBP5 expression and activated the apoptosis and protein tyrosine kinase signaling pathways. Furthermore, adding supplemental GEN to the diet of hens promoted the glycolysis process in the embryonic liver through the insulin-signaling pathway, upregulated target genes (phosphoglucomutase-2, hexokinase 1, dihydroxyacetone phosphate by aldolase, phosphofructokinase, platelet, and enolase 2), and enhanced the transport of carboxylic acids and cholesterol and the synthesis of unsaturated fatty acid (arachidonic acid) in the embryonic liver through upregulation of liver X receptor, sterol regulatory element-binding protein 1, and patatin-like phospholipase A. Additionally, GEN treatment increased fatty acid β-oxidation and Na + /K + -ATPase activity in the embryonic liver through activation of peroxisome proliferator-activated receptors (PPARs; PPARα and PPARδ) and the AMPK signaling pathway, which could provide energy for embryonic development. In addition, GEN treatment in hens increased superoxide dismutase activity and metallothionein expression in the chick embryonic liver and promoted lymphocyte proliferation through upregulation of mRNA expression of CDKN1A, IL12RB1, Sox11, PRKAR1A, PRKCQ, and TCF3. The improved immunity and antioxidant capacity, as a result of maternal GEN effects, was conducive to embryonic development. In conclusion, the addition of GEN to the diet of laying broiler breeder hens significantly promoted the development and metabolism of chick embryos.-Lv, Z., Fan, H., Zhang, B., Ning, C., Xing, K., Guo, Y. Dietary genistein supplementation in laying broiler breeder hens alters the development and metabolism of offspring embryos as revealed by hepatic transcriptome analysis.

Optimal and Local Connectivity Between Neuron and Synapse Array in the Quantum Dot/Silicon Brain

NASA Technical Reports Server (NTRS)

Duong, Tuan A.; Assad, Christopher; Thakoor, Anikumar P.

2010-01-01

This innovation is used to connect between synapse and neuron arrays using nanowire in quantum dot and metal in CMOS (complementary metal oxide semiconductor) technology to enable the density of a brain-like connection in hardware. The hardware implementation combines three technologies: 1. Quantum dot and nanowire-based compact synaptic cell (50x50 sq nm) with inherently low parasitic capacitance (hence, low dynamic power approx.l0(exp -11) watts/synapse), 2. Neuron and learning circuits implemented in 50-nm CMOS technology, to be integrated with quantum dot and nanowire synapse, and 3. 3D stacking approach to achieve the overall numbers of high density O(10(exp 12)) synapses and O(10(exp 8)) neurons in the overall system. In a 1-sq cm of quantum dot layer sitting on a 50-nm CMOS layer, innovators were able to pack a 10(exp 6)-neuron and 10(exp 10)-synapse array; however, the constraint for the connection scheme is that each neuron will receive a non-identical 10(exp 4)-synapse set, including itself, via its efficacy of the connection. This is not a fully connected system where the 100x100 synapse array only has a 100-input data bus and 100-output data bus. Due to the data bus sharing, it poses a great challenge to have a complete connected system, and its constraint within the quantum dot and silicon wafer layer. For an effective connection scheme, there are three conditions to be met: 1. Local connection. 2. The nanowire should be connected locally, not globally from which it helps to maximize the data flow by sharing the same wire space location. 3. Each synapse can have an alternate summation line if needed (this option is doable based on the simple mask creation). The 10(exp 3)x10(exp 3)-neuron array was partitioned into a 10-block, 10(exp 2)x10(exp 3)-neuron array. This building block can be completely mapped within itself (10,000 synapses to a neuron).

Observations of the Minor Species Al and Fe in Mercury's Exosphere

NASA Technical Reports Server (NTRS)

Bida, Thomas A.; Killen, Rosemary M.

2016-01-01

We report here on the first observational evidence of Al and Fe in the exosphere of Mercury, based on measurements of resolved emission lines of these metals with Keck-1/HIRES. Al emission was observed on two separate runs, in 2008 and 2013, with tangent column densities of 3.1 +/- 1.0 and 4.0 +/-1.5 x 10(exp 7) Al atoms cm(exp - 2) at altitudes of 1185 and 1870 km (1.5 and 1.75 R(sub M). The Al radiative intensity was seen to increase where the slit crossed the planetary penumbral shadow, and then decrease monotonically with altitude. Fe emission has been observed once, in 2009, indicating an extended source. We also present observed 3- Sigma Ca(+) upper limits near Mercury's equatorial anti-solar limb, from which an abundance limit of 4.0 x 10(exp 6) cm(exp -2) at 1650 km altitude is derived for the Ca ion. A simple model for zenith column abundances of the neutral species yields 1.9 -5.2 x 10(exp 7) Al cm(exp -2) , and 8.2 x 10(exp 8) Fe cm(exp -2) . The observations appear to be consistent with production of these species by impact vaporization, with a large fraction of the Al ejecta in molecular form, and that for Fe in mixed atomic and molecular forms. The scale height of the Al gas is consistent with a kinetic temperature of 6100-8000 K. The apparent high temperature and low density of the Al gas would suggest that it may be produced by dissociation of molecules.

Ethanol and Acetic Acid Production from Carbon Monoxide in a Clostridium Strain in Batch and Continuous Gas-Fed Bioreactors

PubMed Central

Nalakath Abubackar, Haris; Veiga, María C.; Kennes, Christian

2015-01-01

The effect of different sources of nitrogen as well as their concentrations on the bioconversion of carbon monoxide to metabolic products such as acetic acid and ethanol by Clostridium autoethanogenum was studied. In a first set of assays, under batch conditions, either NH4Cl, trypticase soy broth or yeast extract (YE) were used as sources of nitrogen. The use of YE was found statistically significant (p < 0.05) on the product spectrum in such batch assays. In another set of experiments, three bioreactors were operated with continuous CO supply, in order to estimate the effect of running conditions on products and biomass formation. The bioreactors were operated under different conditions, i.e., EXP1 (pH = 5.75, YE 1g/L), EXP2 (pH = 4.75, YE 1 g/L) and EXP3 (pH = 5.75, YE 0.2 g/L). When compared to EXP2 and EXP3, it was found that EXP1 yielded the maximum biomass accumulation (302.4 mg/L) and products concentrations, i.e., acetic acid (2147.1 mg/L) and ethanol (352.6 mg/L). This can be attributed to the fact that the higher pH and higher YE concentration used in EXP1 stimulated cell growth and did, consequently, also enhance metabolite production. However, when ethanol is the desired end-product, as a biofuel, the lower pH used in EXP2 was more favourable for solventogenesis and yielded the highest ethanol/acetic acid ratio, reaching a value of 0.54. PMID:25608591

Ethanol and acetic acid production from carbon monoxide in a Clostridium strain in batch and continuous gas-fed bioreactors.

PubMed

Abubackar, Haris Nalakath; Veiga, María C; Kennes, Christian

2015-01-20

The effect of different sources of nitrogen as well as their concentrations on the bioconversion of carbon monoxide to metabolic products such as acetic acid and ethanol by Clostridium autoethanogenum was studied. In a first set of assays, under batch conditions, either NH4Cl, trypticase soy broth or yeast extract (YE) were used as sources of nitrogen. The use of YE was found statistically significant (p < 0.05) on the product spectrum in such batch assays. In another set of experiments, three bioreactors were operated with continuous CO supply, in order to estimate the effect of running conditions on products and biomass formation. The bioreactors were operated under different conditions, i.e., EXP1 (pH = 5.75, YE 1g/L), EXP2 (pH = 4.75, YE 1 g/L) and EXP3 (pH = 5.75, YE 0.2 g/L). When compared to EXP2 and EXP3, it was found that EXP1 yielded the maximum biomass accumulation (302.4 mg/L) and products concentrations, i.e., acetic acid (2147.1 mg/L) and ethanol (352.6 mg/L). This can be attributed to the fact that the higher pH and higher YE concentration used in EXP1 stimulated cell growth and did, consequently, also enhance metabolite production. However, when ethanol is the desired end-product, as a biofuel, the lower pH used in EXP2 was more favourable for solventogenesis and yielded the highest ethanol/acetic acid ratio, reaching a value of 0.54.

The Most Powerful Flaring Activity from the NLSy1 PMN J0948+0022

NASA Technical Reports Server (NTRS)

D'Ammando, F.; Orienti, M.; Finke, J.; Raiteri, C. M.; Hovatta, T.; Larsson, J.; Max-Moerbeck, W.; Perkins, J.; Readhead, A. C. S.; Richards, J. L.

2014-01-01

We report on multifrequency observations performed during 2012 December-2013 August of the first narrow-line Seyfert 1 galaxy detected in gamma rays, PMN J0948+0022 (z equal to 0.5846). A gamma-ray flare was observed by the Large Area Telescope on board Fermi during 2012 December-2013 January, reaching a daily peak flux in the 0.1-100 GeV energy range of (155 plus or minus 31)×10(exp -8) ph cm (exp -2) s (exp -1) on 2013 January 1, corresponding to an apparent isotropic luminosity of approximately 1.5×10)exp 48) erg s(exp -1). The gamma-ray flaring period triggered Swift and VERITAS observations in addition to radio and optical monitoring by OVRO, MOJAVE, and CRTS. A strong flare was observed in optical, UV, and X- rays on 2012 December 30, quasi-simultaneously to the gamma-ray flare, reaching a record flux for this source from optical to gamma rays. VERITAS observations at very high energy (E greater than 100 GeV) during 2013 January 6-17 resulted in an upper limit of F(sub greater than 0.2 TeV) less than 4.0 × 10(exp -12) ph cm(exp -2) s(exp -1). We compared the spectral energy distribution (SED) of the flaring state in 2013 January with that of an intermediate state observed in 2011. The two SEDs, modelled as synchrotron emission and an external Compton scattering of seed photons from a dust torus, can be modelled by changing both the electron distribution parameters and the magnetic field.

[Effects of genistein on contractility of isolated right ventricular muscles in guinea pig].

PubMed

Wu, Jin-xia; Li, Hong-fang; Liu, Chong-bin; Tian, Zhi-feng

2008-11-01

To study the effect of genistein (GEN) on contractility of isolated right ventricular muscles in guinea pig and its mechanisms. Isolated guinea pig ventricular muscles were suspended in organ baths containing K-H solution.After an equilibration period, the effect of GEN on contraction of myocardium was observed. GEN and isoprenaline hydrochloride had the positive inotropic effects on contractity of myocardium. Meanwhile, the effect of GEN (1-100 micromol x L(-1)) was in dose-dependent manner. Propranolol (1 micromol x L(-1)) and verapamil hydrochloride (0.5 micromol x L(-1)) attenuated the positive inotropic effect of isoprenaline hydrochloride (1 micromol x L(-1)), but did not change the effect of GEN (50 micromol x L(-1)). Further more, the enhancement of the contraction induced by elevation of extracellular Ca2+ concentration in ventricular muscles had no change after pretreatment with GEN (1.10 micromol x L(-1)). In addition,the positive inotropic effect of GEN was inhibited partially by tamoxifen (1 micromol x L(-1)) and SQ22536 (1 micromol x L(-1)), also, could be attenuated by bpV (1 micromol x L(-1)). GEN has the positive inotropic effect on guinea pig ventricular muscles, which is not related to the activation of beta adrenoceptor, Ca2+ channel on cell membrane,but may involve in cAMP of intracellular signal transduction and tyrosine kinase pathway.

RxGen General Optical Model Prescription Generator

NASA Technical Reports Server (NTRS)

Sigrist, Norbert

2012-01-01

RxGen is a prescription generator for JPL's in-house optical modeling software package called MACOS (Modeling and Analysis for Controlled Optical Systems), which is an expert optical analysis software package focusing on modeling optics on dynamic structures, deformable optics, and controlled optics. The objectives of RxGen are to simplify and automate MACOS prescription generations, reducing errors associated with creating such optical prescriptions, and improving user efficiency without requiring MACOS proficiency. RxGen uses MATLAB (a high-level language and interactive environment developed by MathWorks) as the development and deployment platform, but RxGen can easily be ported to another optical modeling/analysis platform. Running RxGen within the modeling environment has the huge benefit that variations in optical models can be made an integral part of the modeling state. For instance, optical prescription parameters determined as external functional dependencies, optical variations by controlling the in-/exclusion of optical components like sub-systems, and/or controlling the state of all components. Combining the mentioned capabilities and flexibilities with RxGen's optical abstraction layer completely eliminates the hindering aspects for requiring proficiency in writing/editing MACOS prescriptions, allowing users to focus on the modeling aspects of optical systems, i.e., increasing productivity and efficiency. RxGen provides significant enhancements to MACOS and delivers a framework for fast prototyping as well as for developing very complex controlled optical systems.

Gelidium elegans Regulates the AMPK-PRDM16-UCP-1 Pathway and Has a Synergistic Effect with Orlistat on Obesity-Associated Features in Mice Fed a High-Fat Diet.

PubMed

Choi, Jia; Kim, Kui-Jin; Koh, Eun-Jeong; Lee, Boo-Yong

2017-03-30

The incidence of obesity is rising at an alarming rate throughout the world and is becoming a major public health concern with incalculable social and economic costs. Gelidium elegans (GENS), also previously known as Gelidium amansii , has been shown to exhibit anti-obesity effects. Nevertheless, the mechanism by which GENS is able to do this remains unclear. In the present study, our results showed that GENS prevents high-fat diet (HFD)-induced weight gain through modulation of the adenosine monophosphate-activated protein kinase (AMPK)-PR domain-containing16 (PRDM16)-uncoupling protein-1 (UCP-1) pathway in a mice model. We also found that GENS decreased hyperglycemia in mice that had been fed a HFD compared to corresponding controls. We also assessed the beneficial effect of the combined treatment with GENS and orlistat (a Food and Drug Administration-approved obesity drug) on obesity characteristics in HFD-fed mice. We found that in HFD-fed mice, the combination of GENS and orlistat is associated with more significant weight loss than orlistat treatment alone. Moreover, our results demonstrated a positive synergistic effect of GENS and orlistat on hyperglycemia and plasma triglyceride level in these animals. Thus, we suggest that a combination therapy of GENS and orlistat may positively influence obesity-related health outcomes in a diet-induced obese population.

Gelidium elegans Regulates the AMPK-PRDM16-UCP-1 Pathway and Has a Synergistic Effect with Orlistat on Obesity-Associated Features in Mice Fed a High-Fat Diet

PubMed Central

Choi, Jia; Kim, Kui-Jin; Koh, Eun-Jeong; Lee, Boo-Yong

2017-01-01

The incidence of obesity is rising at an alarming rate throughout the world and is becoming a major public health concern with incalculable social and economic costs. Gelidium elegans (GENS), also previously known as Gelidium amansii, has been shown to exhibit anti-obesity effects. Nevertheless, the mechanism by which GENS is able to do this remains unclear. In the present study, our results showed that GENS prevents high-fat diet (HFD)-induced weight gain through modulation of the adenosine monophosphate-activated protein kinase (AMPK)-PR domain-containing16 (PRDM16)-uncoupling protein-1 (UCP-1) pathway in a mice model. We also found that GENS decreased hyperglycemia in mice that had been fed a HFD compared to corresponding controls. We also assessed the beneficial effect of the combined treatment with GENS and orlistat (a Food and Drug Administration-approved obesity drug) on obesity characteristics in HFD-fed mice. We found that in HFD-fed mice, the combination of GENS and orlistat is associated with more significant weight loss than orlistat treatment alone. Moreover, our results demonstrated a positive synergistic effect of GENS and orlistat on hyperglycemia and plasma triglyceride level in these animals. Thus, we suggest that a combination therapy of GENS and orlistat may positively influence obesity-related health outcomes in a diet-induced obese population. PMID:28358328

An evaluation of the accuracy of the ORange (Gen II) by comparing it to the IOLMaster in the prediction of postoperative refraction.

PubMed

Chen, Ming

2012-01-01

The aim of this study was to evaluate the accuracy of ORange(®) Gen II (WaveTec Vision, Aliso Viejo, CA). The Surgical Suites, Honolulu, HI. The prospective 28 consecutive cataract surgical cases were selected from 85 cataract surgical cases between December 16, 2010 and February 24, 2011. With the same intraocular lens implantation, the predicted spherical equivalent refraction from IOLMaster(®) (Carl Zeiss AG, Oberkochen, Germany) and ORange Gen II were statistically compared and verified with 1-month postoperative manifest refraction. The data were put into IBM SPSS 19 (SPSS Inc, Chicago, IL) for analysis of variance. Pearson's correlation coefficient was also calculated to evaluate the correlation between the IOLMaster, ORange Gen II, and 1-month postoperative manifest refraction. There were no statistically significant differences in the mean spherical equivalent refraction from the IOLMaster, ORange Gen II, and 1-month postoperative manifest refraction (IOLMaster -0.40 diopters, P = 0.07; ORange Gen II -0.43 diopters, P = 0.16; 1-month refraction -0.41 diopters, P = 0.07). Pearson's correlation study demonstrated that all three were positively correlated (P < 0.05), with the strongest correlation between the ORange Gen II and 1-month postoperative manifest refraction (r = +0.6, P < 0.01). The ORange Gen II can be considered as an alternative method for intraocular lens selection for cataract patients.

ERRATUM: Papers published in incorrect sections

NASA Astrophysics Data System (ADS)

2004-04-01

A number of J. Phys. A: Math. Gen. articles have mistakenly been placed in the wrong subject section in recent issues of the journal. We would like to apologize to the authors of these articles for publishing their papers in the Fluid and Plasma Theory section. The correct section for each article is given below. Statistical Physics Issue 4: Microcanonical entropy for small magnetizations Behringer H 2004 J. Phys. A: Math. Gen. 37 1443 Mathematical Physics Issue 9: On the solution of fractional evolution equations Kilbas A A, Pierantozzi T, Trujillo J J and Vázquez L 2004 J. Phys. A: Math. Gen. 37 3271 Quantum Mechanics and Quantum Information Theory Issue 6: New exactly solvable isospectral partners for PT-symmetric potentials Sinha A and Roy P 2004 J. Phys. A: Math. Gen. 37 2509 Issue 9: Symplectically entangled states and their applications to coding Vourdas A 2004 J. Phys. A: Math. Gen. 37 3305 Classical and Quantum Field Theory Issue 6: Pairing of parafermions of order 2: seniority model Nelson C A 2004 J. Phys. A: Math. Gen. 37 2497 Issue 7: Jordan-Schwinger map, 3D harmonic oscillator constants of motion, and classical and quantum parameters characterizing electromagnetic wave polarization Mota R D, Xicoténcatl M A and Granados V D 2004 J. Phys. A: Math. Gen. 37 2835 Issue 9: Could only fermions be elementary? Lev F M 2004 J. Phys. A: Math. Gen. 37 3285

Perinatal exposure to genistein, a soy phytoestrogen, improves spatial learning and memory but impairs passive avoidance learning and memory in offspring.

PubMed

Kohara, Yumi; Kuwahara, Rika; Kawaguchi, Shinichiro; Jojima, Takeshi; Yamashita, Kimihiro

2014-05-10

This study investigated the effects of perinatal genistein (GEN) exposure on the central nervous system of rat offspring. Pregnant dams orally received GEN (1 or 10 mg/kg/day) or vehicle (1 ml/kg/day) from gestation day 10 to postnatal day 14. In order to assess the effects of GEN on rat offspring, we used a battery of behavioral tests, including the open-field, elevated plus-maze, MAZE and step-through passive avoidance tests. MAZE test is an appetite-motivation test, and we used this mainly for assessing spatial learning and memory. In the MAZE test, GEN groups exhibited shorter latency from start to goal than the vehicle-treated group in both sexes. On the other hand, performances in the step-through passive avoidance test were non-monotonically inhibited by GEN in both sexes, and a significant difference was observed in low dose of the GEN-treated group compared to the vehicle-treated group in female rats. Furthermore, we found that perinatal exposure to GEN did not significantly alter locomotor activity or emotionality as assessed by the open-field and elevated-plus maze tests. These results suggest that perinatal exposure to GEN improved spatial learning and memory of rat offspring, but impaired their passive avoidance learning and memory. Copyright © 2014 Elsevier Inc. All rights reserved.

Reynolds Number Effects on the Performance of Lateral Control Devices

NASA Technical Reports Server (NTRS)

Mineck, Raymond E.

2000-01-01

The influence of Reynolds number on the performance of outboard spoilers and ailerons was investigated on a generic subsonic transport configuration in the National Transonic Facility over a chord Reynolds number range 41 from 3x10(exp 6) to 30xl0(exp 6) and a Mach number range from 0.50 to 0.94, Spoiler deflection angles of 0, 10, 15, and 20 deg and aileron deflection angles of -10, 0, and 10 deg were tested. Aeroelastic effects were minimized by testing at constant normalized dynamic pressure conditions over intermediate Reynolds number ranges. Results indicated that the increment in rolling moment due to spoiler deflection generally becomes more negative as the Reynolds number increases from 3x10(exp 6) to 22x10(exp 6) with only small changes between Reynolds numbers of 22x10(exp 6) and 30x10(exp 6). The change in the increment in rolling moment coefficient with Reynolds number for the aileron deflected configuration is generally small with a general trend of increasing magnitude with increasing Reynolds number.

Relic gravitational waves and extended inflation

NASA Technical Reports Server (NTRS)

Turner, Michael S.; Wilczek, Frank

1990-01-01

In extended inflation, a new version of inflation where the transition from the false-vacuum phase to a radiation-dominated Universe is accomplished by bubble nucleation and percolation, bubble collisions supply a potent-and potentially detectable-source of gravitational waves. The present energy density in relic gravity waves from bubble collisions is expected to be about 10(exp -5) of closure density-many orders of magnitude greater than that of the gravity waves produced by quantum fluctuations. Their characteristic wavelength depends upon the reheating temperature T(sub RH): lambda is approximately 10(exp 4) cm (10(exp 14) GeV/T(sub RH)). If large numbers of black holes are produced, a not implausible outcome, they will evaporate producing comparable amounts of shorter wavelength waves, lambda is approximately 10(exp -6) cm (T(sub RH)/10(exp 14) GeV).

Expanded ATXN3 frameshifting events are toxic in Drosophila and mammalian neuron models.

PubMed

Stochmanski, Shawn J; Therrien, Martine; Laganière, Janet; Rochefort, Daniel; Laurent, Sandra; Karemera, Liliane; Gaudet, Rebecca; Vyboh, Kishanda; Van Meyel, Don J; Di Cristo, Graziella; Dion, Patrick A; Gaspar, Claudia; Rouleau, Guy A

2012-05-15

Spinocerebellar ataxia type 3 is caused by the expansion of the coding CAG repeat in the ATXN3 gene. Interestingly, a -1 bp frameshift occurring within an (exp)CAG repeat would henceforth lead to translation from a GCA frame, generating polyalanine stretches instead of polyglutamine. Our results show that transgenic expression of (exp)CAG ATXN3 led to -1 frameshifting events, which have deleterious effects in Drosophila and mammalian neurons. Conversely, transgenic expression of polyglutamine-encoding (exp)CAA ATXN3 was not toxic. Furthermore, (exp)CAG ATXN3 mRNA does not contribute per se to the toxicity observed in our models. Our observations indicate that expanded polyglutamine tracts in Drosophila and mouse neurons are insufficient for the development of a phenotype. Hence, we propose that -1 ribosomal frameshifting contributes to the toxicity associated with (exp)CAG repeats.

Temperature-Dependent Kinetics Studies of the Reactions Br((sup 2)P3/2) + H2S yields SH + HBr and Br((sup 2)P3/2) + CH3SH yields CH3S + HBr. Heats of Formation of SH and CH3S Radicals

NASA Technical Reports Server (NTRS)

Nicovich, J. M.; Kreutter, K. D.; vanDijk, C. A.; Wine, P. H.

1997-01-01

Time resolved resonance fluorescence detection of Br(sup 2)P3/2) atom disappearance or appearance following 266-nm laser flash photolysis of CF2Br2/H2S/H2/N2, CF2Br2/CH3SH/H2/N2, Cl2CO/H2S/HBr/N2, and CH3SSCH3/HBr/H2/N2 mixtures has been employed to study the kinetics of the reactions Br((sup 2)P3/2) + H2S = SH + HBr (1,-1) and Br((sup2)P3/2) + CH3SH = CH3S + HBr (2, -2) as a function of temperature over the range 273-431K. Arrhenius expressions in units of 10(exp -12) cu cm/molecule/s which describe the results are k1 = (14.2 +/- 3.4) exp[(-2752 +/- 90)/T],(k-1) = (4.40 +/- 0.92) exp[(-971 +/- 73)/T],k(2) = (9.24 +/- 1.15) exp[(-386 +/- 41)/T], and k(-2) = (1.46 +/-0.21) exp[(-399 +/-41)/T; errors are 2 sigma and represent precision only. By examining Br((sup 2)P3/2) equilibrium kinetics following 355nm laser flash photolysis of Br2/CH3SH/H2/N2 mixtures, a 298 K rate coefficient of (1.7 +/- 0.5) x 10(exp -10) cu cm/molecule/s has been obtained for the reaction CH3S + Br2 yields CH3SBr + Br. To our knowledge, these are the first kinetic data reported for each of the reactions studied. Measured rate coefficients, along with known rate coefficients for similar radical + H2S, CH3SH, HBr,Br2 reactions are considered in terms of possible correlations of reactivity with reaction thermochemistry and with IP - EA, the difference between the ionization potential of the electron donor and the electron affinity of the electron acceptor. Both thermochemical and charge-transfer effects appear to be important in controlling observed reactivities. Second and third law analyses of the equilibrium data for reactions 1 and 2 have been employed to obtain the following enthalpies of reaction in units of kcal/mol: for reaction 1, Delta-H(298) = 3.64 +/- 0.43 and Delta-H(0) = 3.26 +/-0.45; for reaction 2, Delta-H(298) = -0.14 +/- 0.28 and Delta-H(0) = -0.65 +/- 0.36. Combining the above enthalpies of reaction with the well-known heats of formation of Br, HBr, H2S, and CH3SH gives the following heats of formation for the RS radicals in units of kcal/mol: Delta-H(sub f)(sub 0)(SH) = 34.07 +/- 0.72, Delta-H(sub f)(sub 298)(SH) = 34.18 +/- 0.68, Delta-H(sub f)(sub 0)(CH3S) = 31.44 +/- 0.54, Delta-H(sub f)(sub 298)(CH3S) = 29.78 +/- 0.44; errors are 2 sigma and represent estimates of absolute accuracy. The SH heat of formation determined from our data agrees well with literature values but has reduced error limits compared to other available values. The CH3S heat of formation determined from our date is near the low end of the range of previous estimates and is 3-4 kcal/mol lower than values derived from recent molecular beam photofragmentation studies.

Efficacy of Ciprofloxacin-Gentamicin Combination Therapy in Murine Bubonic Plague

PubMed Central

Lemaître, Nadine; Ricard, Isabelle; Pradel, Elizabeth; Foligné, Benoît; Courcol, René; Simonet, Michel; Sebbane, Florent

2012-01-01

Potential benefits of combination antibiotic therapy for the treatment of plague have never been evaluated. We compared the efficacy of a ciprofloxacin (CIN) and gentamicin (GEN) combination therapy with that of each antibiotic administered alone (i) against Yersinia pestis in vitro and (ii) in a mouse model of bubonic plague in which animals were intravenously injected with antibiotics for five days, starting at two different times after infection (44 h and 56 h). In vitro, the CIN+GEN combination was synergistic at 0.5x the individual drugs’ MICs and indifferent at 1x- or 2x MIC. In vivo, the survival rate for mice treated with CIN+GEN was similar to that observed with CIN alone and slightly higher than that observed for GEN alone 100, 100 and 85%, respectively when treatment was started 44 h post challenge. 100% of survivors were recorded in the CIN+GEN group vs 86 and 83% in the CIN and GEN groups, respectively when treatment was delayed to 56 h post-challenge. However, these differences were not statistically significant. Five days after the end of treatment, Y. pestis were observed in lymph nodes draining the inoculation site (but not in the spleen) in surviving mice in each of the three groups. The median lymph node log10 CFU recovered from persistently infected lymph nodes was significantly higher with GEN than with CIN (5.8 vs. 3.2, p = 0.04) or CIN+GEN (5.8 vs. 2.8, p = 0.01). Taken as the whole, our data show that CIN+GEN combination is as effective as CIN alone but, regimens containing CIN are more effective to eradicate Y. pestis from the draining lymph node than the recommended GEN monotherapy. Moreover, draining lymph nodes may serve as a reservoir for the continued release of Y. pestis into the blood – even after five days of intravenous antibiotic treatment. PMID:23285069

Efficacy of ciprofloxacin-gentamicin combination therapy in murine bubonic plague.

PubMed

Lemaître, Nadine; Ricard, Isabelle; Pradel, Elizabeth; Foligné, Benoît; Courcol, René; Simonet, Michel; Sebbane, Florent

2012-01-01

Potential benefits of combination antibiotic therapy for the treatment of plague have never been evaluated. We compared the efficacy of a ciprofloxacin (CIN) and gentamicin (GEN) combination therapy with that of each antibiotic administered alone (i) against Yersinia pestis in vitro and (ii) in a mouse model of bubonic plague in which animals were intravenously injected with antibiotics for five days, starting at two different times after infection (44 h and 56 h). In vitro, the CIN+GEN combination was synergistic at 0.5x the individual drugs' MICs and indifferent at 1x- or 2x MIC. In vivo, the survival rate for mice treated with CIN+GEN was similar to that observed with CIN alone and slightly higher than that observed for GEN alone 100, 100 and 85%, respectively when treatment was started 44 h post challenge. 100% of survivors were recorded in the CIN+GEN group vs 86 and 83% in the CIN and GEN groups, respectively when treatment was delayed to 56 h post-challenge. However, these differences were not statistically significant. Five days after the end of treatment, Y. pestis were observed in lymph nodes draining the inoculation site (but not in the spleen) in surviving mice in each of the three groups. The median lymph node log(10) CFU recovered from persistently infected lymph nodes was significantly higher with GEN than with CIN (5.8 vs. 3.2, p = 0.04) or CIN+GEN (5.8 vs. 2.8, p = 0.01). Taken as the whole, our data show that CIN+GEN combination is as effective as CIN alone but, regimens containing CIN are more effective to eradicate Y. pestis from the draining lymph node than the recommended GEN monotherapy. Moreover, draining lymph nodes may serve as a reservoir for the continued release of Y. pestis into the blood - even after five days of intravenous antibiotic treatment.

Complete Mitochondrial Genomes of the Cherskii’s Sculpin Cottus czerskii and Siberian Taimen Hucho taimen Reveal GenBank Entry Errors: Incorrect Species Identification and Recombinant Mitochondrial Genome

PubMed Central

Balakirev, Evgeniy S; Saveliev, Pavel A; Ayala, Francisco J

2017-01-01

The complete mitochondrial (mt) genome is sequenced in 2 individuals of the Cherskii’s sculpin Cottus czerskii. A surprisingly high level of sequence divergence (10.3%) has been detected between the 2 genomes of C czerskii studied here and the GenBank mt genome of C czerskii (KJ956027). At the same time, a surprisingly low level of divergence (1.4%) has been detected between the GenBank C czerskii (KJ956027) and the Amur sculpin Cottus szanaga (KX762049, KX762050). We argue that the observed discrepancies are due to incorrect taxonomic identification so that the GenBank accession number KJ956027 represents actually the mt genome of C szanaga erroneously identified as C czerskii. Our results are of consequence concerning the GenBank database quality, highlighting the potential negative consequences of entry errors, which once they are introduced tend to be propagated among databases and subsequent publications. We illustrate the premise with the data on recombinant mt genome of the Siberian taimen Hucho taimen (NCBI Reference Sequence Database NC_016426.1; GenBank accession number HQ897271.1), bearing 2 introgressed fragments (≈0.9 kb [kilobase]) from 2 lenok subspecies, Brachymystax lenok and Brachymystax lenok tsinlingensis, submitted to GenBank on June 12, 2011. Since the time of submission, the H taimen recombinant mt genome leading to incorrect phylogenetic inferences was propagated in multiple subsequent publications despite the fact that nonrecombinant H taimen genomes were also available (submitted to GenBank on August 2, 2014; KJ711549, KJ711550). Other examples of recombinant sequences persisting in GenBank are also considered. A GenBank Entry Error Depositary is urgently needed to monitor and avoid a progressive accumulation of wrong biological information. PMID:28890653

LONG-TERM OCCUPATIONAL AND ENVIRONMENTAL EXPOSURE TO PENCONAZOLE AND TEBUCONAZOLE BY HAIR BIOMONITORING.

PubMed

Mercadante, Rosa; Polledri, Elisa; Moretto, Angelo; Fustinoni, Silvia

2018-06-09

Penconazole (PEN) and tebuconazole (TEB) are fungicides widely used in vineyards. The aim of this the study was to assess the suitability of hair to assess long-term exposure to PEN and TEB. Hair samples of agricultural workers exposed to PEN (AW-PEN, 18 subjects) or TEB (AW-TEB, 2 subjects) during the application of fungicides, agricultural workers relatives (AR, 4 subjects), and research staff technicians (RS, 5 subjects) were collected before (PRE-EXP) and after (POST-EXP) the application season. PEN in PRE-EXP samples was quantifiable in all AW and AR (medians from 1.4 to 7.9 pg/mg hair) and in one RS (1.4 pg/mg hair); PEN in POST-EXP samples was always quantifiable (medians from 2.6 to 23.7 pg/mg hair), with higher levels in AW. Comparing PRE- vs. POST-EXP samples, an increase in PEN level in AW and RS was found. TEB in PRE-EXP samples was quantifiable in most AW and AR (median from 2.1 to 15.5 pg/mg hair), but not in RS; TEB in POST-EXP samples was similarly quantifiable in AW and AR, and was quantifiable also in RS (from 1.4 to median of 141.3 pg/mg hair). Comparing PRE- vs. POST-EXP samples, an increase in TEB level in AW and RS was found. In AW, a positive correlation between the number of PEN treatments during the season and the POST-EXP level of PEN in hair was found (N = 8, Spearman rho = 0.794, p = 0.019). Our results suggest that PEN and TEB accumulate in hair during the agricultural season and that hair is a promising matrix for biomonitoring long-term exposure. Copyright © 2018 Elsevier B.V. All rights reserved.

Effects of various kinds of edible seaweeds in diets on the development of D-galactosamine-induced hepatopathy in rats.

PubMed

Kawano, Naoko; Egashira, Yukari; Sanada, Hiroo

2007-08-01

In the present study we investigated the effects of 11 kinds of edible seaweeds (6 brown and 5 red algae) which contain characteristic seaweed dietary fibers on the induction of D-GalN (D-galactosamine)-hepatopathy in rats (Exps. 1 and 2). Then, the efficacy of various components prepared from Gelidium sp., which was found to alleviate the hepatopathy in Exps. 1 and 2, was examined (Exp. 3). The rats were fed the diets containing various kinds of seaweeds (Exps. 1 and 2), or several components of Gelidium sp. such as total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF) and dietary fiber-free components (DFFC) (Exp. 3), for 8 d. The rats in all experiments were injected with D-GalN (800 mg/kg body weight) intraperitoneally at the 7th day to induce liver injury and were sacrificed 24 h after the injection of D-GalN. The serum transaminase activities (ALT and AST) and lactate dehydrogenase (LDH) were determined to evaluate the levels of hepatopathy. In Exp. 3, the total GSH concentration in the liver, plasma and cecal contents and organic acid concentration in cecal contents were also evaluated. In Exps. 1 and 2, repressive effects against D-GalN-hepatopathy were shown by four seaweeds Laminaria sp., Gelidium sp., Sargassum fulvellum and Eisenia bicyclis. In Exp. 3, it was found that protective activity in Gelidium sp. against D-GalN-hepatopathy existed not only in the SDF but also in the DFFC fraction. The results in Exp. 3 also indicated that the total GSH but not organic acid concentration in the cecal contents were significantly correlated with serum AST activity, suggesting that the protective effect of Gelidium sp. on D-GalN-hepatopathy in rats is related to GSH metabolism in the intestine.

Photoionization of the diffuse interstellar medium and galactic halo by OB associtations

NASA Technical Reports Server (NTRS)

Dove, James B.; Shull, J. Michael

1994-01-01

Assuming smoothly varying H I distributions in te Galactic disk, we have calculated the geometry of diffuse II regions due to OB associations in the Galactic plane. Near the solar circle, OB associations with a Lyman continuum (Lyc) photon luminosity Psi(sub Lyc) = 3.3 x 10(exp 7) cm(exp -2) s(exp -1), produce H II regions that are density bounded in the vertical direction (H II chimneys) allowing Lyc to escape the gaseous disk and penetrate into the Galactic halo. We provide analytic formulae for the Lyc escape fraction as functions of S(sub 0) O-star catalog of Garmany and a new Lyc stellar stellar Lyc stellar flux calibration, we find a production rate of Lyc photons by OB associations within 2.5 kpc of Psi(sub Lyc) = 3.3 x 10(exp 7) cm(exp -2) s(exp -1). Integrating the fraction of Lyc photons that escape the disk over our adopted luminosity function of OB associations, we estimate that approximately 7% of the ionizing photons, or Phi(sub Lyc) = 2.3 x 10(exp 6) cm(exp -2) s(exp -1), escape each side of the H I disk layer and penetrate the diffuse ionized medium ('Reynolds layer'). This flux is sufficient to explain the potoionization of this, although we have not constructed a model for the observed H-alpha emission and pulsar dispersion measures that is fully consistent with the absorption rate of Lyc in the H II layer. Since our quiescent model does not account for the effects of dynamic chimneys and superbubbles, which should enhance Lyc escape, we conclude the O stars are the probable source of ionizing radiation for the Reynolds layer. For a random distribution of OB associations throughout the disk, the Lyc flux is nearly uniform for heights Z is greater than approximately 0.8 kpc above the midplane.

Spring-Summer Temperatures Since AD 1780 Reconstructed from Stable Oxygen Isotope Ratios in White Spruce Tree-Rings from the Mackenzie Delta, Northwestern Canada

NASA Technical Reports Server (NTRS)

Porter, Trevor J.; Pisaric, Michael F. J.; Field, Robert D.; Kokelj, Steven V.; Edwards, Thomas W. D.; deMontigny, Peter; Healy, Richard; LeGrande, Allegra N.

2013-01-01

High-latitude delta(exp 18)O archives deriving from meteoric water (e.g., tree-rings and ice-cores) can provide valuable information on past temperature variability, but stationarity of temperature signals in these archives depends on the stability of moisture source/trajectory and precipitation seasonality, both of which can be affected by atmospheric circulation changes. A tree-ring delta(exp 18)O record (AD 1780-2003) from the Mackenzie Delta is evaluated as a temperature proxy based on linear regression diagnostics. The primary source of moisture for this region is the North Pacific and, thus, North Pacific atmospheric circulation variability could potentially affect the tree-ring delta(exp 18)O-temperature signal. Over the instrumental period (AD 1892-2003), tree-ring delta(exp 18)O explained 29% of interannual variability in April-July minimum temperatures, and the explained variability increases substantially at lower-frequencies. A split-period calibration/verification analysis found the delta(exp 18)O-temperature relation was time-stable, which supported a temperature reconstruction back to AD 1780. The stability of the delta(exp 18)O-temperature signal indirectly implies the study region is insensitive to North Pacific circulation effects, since North Pacific circulation was not constant over the calibration period. Simulations from the NASA-GISS ModelE isotope-enabled general circulation model confirm that meteoric delta(exp 18)O and precipitation seasonality in the study region are likely insensitive to North Pacific circulation effects, highlighting the paleoclimatic value of tree-ring and possibly other delta(exp 18)O records from this region. Our delta(exp 18)O-based temperature reconstruction is the first of its kind in northwestern North America, and one of few worldwide, and provides a long-term context for evaluating recent climate warming in the Mackenzie Delta region.

The Composition of Titan's Lower Atmosphere and Simple Surface Volatiles as Measured by the Cassini-Huygens Probe Gas Chromatograph Mass Spectrometer Experiment

NASA Technical Reports Server (NTRS)

Niemann, H. B.; Atreya, S. K.; Demick, J. E.; Gautier, D.; Haberman, J. A.; Harpold, D. N.; Kasprzak, W. T.; Lunine, J. I.; Owen, T. C.; Raulin, F.

2010-01-01

The Cassini-Huygens Probe Gas Chromatograph Mass Spectrometer (GCMS) determined the composition of the Titan atmosphere from 140km altitude to the surface. After landing, it returned composition data of gases evaporated from the surface. Height profiles of molecular nitrogen (N2), methane (CH4) and molecular hydrogen (H2) were determined. Traces were detected on the surface of evaporating methane, ethane (C2H6), acetylene (C2H2), cyanogen (C2N2) and carbon dioxide (CO2). The methane data showed evidence that methane precipitation occurred recently. The methane mole fraction was (1.48+/-0.09) x 10(exp -2) in the lower stratosphere (139.8 km to 75.5 km) and (5.65+/-0.18) x 10(exp -2) near the surface (6.7 km to the surface). The molecular hydrogen mole fraction was (1.01+/-0.16) x 10(exp -3) in the atmosphere and (9.90+/-0.17) x 10(exp -4) on the surface. Isotope ratios were 167.7+/-0.6 for N-14/N-15 in molecular nitrogen, 91.1+/-1.4 for C-12/C-13 in methane and (1.35+/-0.30) x 10(exp -4) for D/H in molecular hydrogen. The mole fractions of Ar-36 and radiogenic Ar-40 are (2.1+/-0.8) x 10(exp -7) and (3.39 +/-0.12) x 10(exp -5) respectively. Ne-22 has been tentatively identified at a mole fraction of (2.8+/-2.1) x 10(exp -7) Krypton and xenon were below the detection threshold of 1 x 10(exp -8) mole fraction. Science data were not retrieved from the gas chromatograph subsystem as the abundance of the organic trace gases in the atmosphere and on the ground did not reach the detection threshold. Results previously published from the GCMS experiment are superseded by this publication.

The X-ray Spectrum of the North Polar Spur

NASA Technical Reports Server (NTRS)

Willingale, Richard; Hands, A. D. P.; Warwick, R. S.; Snowden, S. L.; Burrows, David N.

2003-01-01

An analysis is presented of the soft X-ray background spectrum measured by the EPIC MOS cameras on XMM-Newton in three observations targeted on the North Polar Spur (NPS). Three distinct Galactic plasma components are identified, a cool Local Hot Bubble (LHB) component, T(sub lo) approx. 0.1 keV, a cool Galactic Halo component at a similar temperature and a hotter component, T(sub hi) approx. 0.26 keV, associated with the NPS itself. Using the new data in combination with the Rosat All-Sky Survey count rates measured in the 0.1-0.4 keV band, we estimate the emission measure of the LHB material to be 0.0040-0.0052 cm(exp -6) pc, which implies an electron density of 0.008-0.011 cm(exp -3) and pressure of approx. 22000 cm(exp -3) K. The halo and NPS components lie behind at least 50% of the line-of-sight cold gas for which the total Galactic column density is in the range (2 - 8) x 10(exp 20) cm(exp -2). Modelling the X-ray emitting superbubble as a sphere at distance 210 pc, radius 140 pc and center l(sub II) = 352 deg, b(sub II) = 15 deg, the implied electron density in the NPS is approx. 0.03 cm(exp -3) with pressure approx. 150000 cm(exp -3) K. The observed spectral line complexes from OVII, OVIII, FeXVII, NeIX, NeX and MgXI provide constraints on the composition of the plasma. The hot component in the NPS is depleted in oxygen, neon and, to some extent, magnesium and iron. Assuming the effective line of sight across the halo emission is 1 kpc, the electron density in the halo is 0.007-0.011 cm(exp -3) and the pressure is approx. 16500 cm(exp -3) K, conditions very similar to those in the LHB.

Identification and Characterization of Key Human Performance Issues and Research in the Next Generation Air Transportation System (NextGen)

NASA Technical Reports Server (NTRS)

Lee, Paul U.; Sheridan, Tom; Poage, james L.; Martin, Lynne Hazel; Jobe, Kimberly K.

2010-01-01

This report identifies key human-performance-related issues associated with Next Generation Air Transportation System (NextGen) research in the NASA NextGen-Airspace Project. Four Research Focus Areas (RFAs) in the NextGen-Airspace Project - namely Separation Assurance (SA), Airspace Super Density Operations (ASDO), Traffic Flow Management (TFM), and Dynamic Airspace Configuration (DAC) - were examined closely. In the course of the research, it was determined that the identified human performance issues needed to be analyzed in the context of NextGen operations rather than through basic human factors research. The main gaps in human factors research in NextGen were found in the need for accurate identification of key human-systems related issues within the context of specific NextGen concepts and better design of the operational requirements for those concepts. By focusing on human-system related issues for individual concepts, key human performance issues for the four RFAs were identified and described in this report. In addition, mixed equipage airspace with components of two RFAs were characterized to illustrate potential human performance issues that arise from the integration of multiple concepts.

Tables of model atmospheres of bursting neutron stars

NASA Technical Reports Server (NTRS)

Madej, Jerzy

1991-01-01

This paper presents tables of plane-parallel neutron star model atmospheres in radiative and hydrostatic equilibrium, with effective temperatures of 8 x 10 exp 6, 1.257 x 10 exp 7, 2 x 10 exp 7, and 3 x 10 exp 7 K, and surface gravities of 15.0 and less (cgs units). The equations of model atmospheres on which the tables are based fully account for nonisotropies of the radiation field and effects of noncoherent Compton scattering of thermal X-rays by free electrons. Both the effective temperatures and gravities listed above are measured on the neutron star surface.

Description of two smallest field crickets from South America, Laureopsis nauta Jaiswara gen. nov., sp. nov. and Perugryllus estiron Jaiswara gen. nov., sp. nov. (Orthoptera, Grylloidea, Gryllidae, Gryllinae).

PubMed

Jaiswara, Ranjana; Desutter-Grandcolas, Laure

2017-11-20

Gryllinae are one of the most diverse and widely distributed cricket groups. However, in South America they are known only from 10 genera. We update this list by describing two new genera and species of field crickets i.e. Laureopsis nauta Jaiswara gen. nov., sp. nov. and Perugryllus estiron Jaiswara gen. nov., sp. nov. from Peru.

Helminth parasites in some Spanish bats.

PubMed

Alvarez, F; Rey, J; Quinteiro, P; Iglesias, R; Santos, M; Sanmartin, M L

1991-01-01

Nineteen bats of the species Rhinolophus ferrumequinum, R. hipposideros, Myotis myotis, M. nattereri, Pipistrellus pipistrellus, Barbastella barbastellus, Eptesicus serotinus and Plecotus auritus captured in N. W. Spain in 1983-85 were found to contain the following helminth parasites: Mesotretes peregrinus (found in 4 host species and making up 31% of all helminths); Plagiorchis vespertilionis (10.5%, in 2 host species); Strongylacantha glycirrhiza, Molinostrongylus alatus, Molineidae gen. sp., Capillariidae gen. sp., Hymenolepis acuta, Cestoda gen. sp. and Trematoda gen. sp. I and II (5.2% in 1 host species).

Complete mitochondrial genome sequences of Atlantic representatives of the invasive Pacific coral species Tubastraea coccinea and T. tagusensis (Scleractinia, Dendrophylliidae): Implications for species identification.

PubMed

Capel, K C C; Migotto, A E; Zilberberg, C; Lin, M F; Forsman, Z; Miller, D J; Kitahara, M V

2016-09-30

Members of the azooxanthellate coral genus Tubastraea are invasive species with particular concern because they have become established and are fierce competitors in the invaded areas in many parts of the world. Pacific Tubastraea species are spreading fast throughout the Atlantic Ocean, occupying over 95% of the available substrate in some areas and out-competing native endemic species. Approximately half of all known coral species are azooxanthellate but these are seriously under-represented compared to zooxanthellate corals in terms of the availability of mitochondrial (mt) genome data. In the present study, the complete mt DNA sequences of Atlantic individuals of the invasive scleractinian species Tubastraea coccinea and Tubastraea tagusensis were determined and compared to the GenBank reference sequence available for a Pacific "T. coccinea" individual. At 19,094bp (compared to 19,070bp for the GenBank specimen), the mt genomes assembled for the Atlantic T. coccinea and T. tagusensis were among the longest sequence determined to date for "Complex" scleractinians. Comparisons of genomes data showed that the "T. coccinea" sequence deposited on GenBank was more closely related to that from Dendrophyllia arbuscula than to the Atlantic Tubastraea spp., in terms of genome length and base pair similarities. This was confirmed by phylogenetic analysis, suggesting that the former was misidentified and might actually be a member from the genus Dendrophyllia. In addition, although in general the COX1 locus has a slow evolutionary rate in Scleractinia, it was the most variable region of the Tubastraea mt genome and can be used as markers for genus or species identification. Given the limited data available for azooxanthellate corals, the results presented here represent an important contribution to our understanding of phylogenetic relationships and the evolutionary history of the Scleractinia. Copyright © 2016 Elsevier B.V. All rights reserved.

Valve-sparing aortic root replacement in patients with Marfan syndrome enrolled in the National Registry of Genetically Triggered Thoracic Aortic Aneurysms and Cardiovascular Conditions.

PubMed

Song, Howard K; Preiss, Liliana R; Maslen, Cheryl L; Kroner, Barbara; Devereux, Richard B; Roman, Mary J; Holmes, Kathryn W; Tolunay, H Eser; Desvigne-Nickens, Patrice; Asch, Federico M; Milewski, Rita K; Bavaria, Joseph; LeMaire, Scott A

2014-05-01

The long-term outcomes of aortic valve-sparing (AVS) root replacement in Marfan syndrome (MFS) patients remain uncertain. The study aim was to determine the utilization and outcomes of AVS root replacement in MFS patients enrolled in the Registry of Genetically Triggered Thoracic Aortic Aneurysms and Cardiovascular Conditions (GenTAC). At the time of this analysis, 788 patients with MFS were enrolled in the GenTAC Registry, of whom 288 had undergone aortic root replacement. Patients who had undergone AVS procedures were compared to those who had undergone aortic valve replacement (AVR). AVS root replacement was performed in 43.5% of MFS patients, and the frequency of AVS was increased over the past five years. AVS patients were younger at the time of surgery (31.0 versus 36.3 years, p = 0.006) and more likely to have had elective rather than emergency surgery compared to AVR patients, in whom aortic valve dysfunction and aortic dissection was the more likely primary indication for surgery. After a mean follow up of 6.2 +/- 3.6 years, none of the 87 AVS patients had required reoperation; in contrast, after a mean follow up of 10.5 +/- 7.6 years, 11.5% of AVR patients required aortic root reoperation. Aortic valve function has been durable, with 95.8% of AVS patients having aortic insufficiency that was graded as mild or less. AVS root replacement is performed commonly among the MFS population, and the durability of the aortic repair and aortic valve function have been excellent to date. These results justify a continued use of the procedure in an elective setting. The GenTAC Registry will be a useful resource to assess the long-term durability of AVS root replacement in the future.

A priori testing of subgrid-scale models for the velocity-pressure and vorticity-velocity formulations

NASA Technical Reports Server (NTRS)

Winckelmans, G. S.; Lund, T. S.; Carati, D.; Wray, A. A.

1996-01-01

Subgrid-scale models for Large Eddy Simulation (LES) in both the velocity-pressure and the vorticity-velocity formulations were evaluated and compared in a priori tests using spectral Direct Numerical Simulation (DNS) databases of isotropic turbulence: 128(exp 3) DNS of forced turbulence (Re(sub(lambda))=95.8) filtered, using the sharp cutoff filter, to both 32(exp 3) and 16(exp 3) synthetic LES fields; 512(exp 3) DNS of decaying turbulence (Re(sub(Lambda))=63.5) filtered to both 64(exp 3) and 32(exp 3) LES fields. Gaussian and top-hat filters were also used with the 128(exp 3) database. Different LES models were evaluated for each formulation: eddy-viscosity models, hyper eddy-viscosity models, mixed models, and scale-similarity models. Correlations between exact versus modeled subgrid-scale quantities were measured at three levels: tensor (traceless), vector (solenoidal 'force'), and scalar (dissipation) levels, and for both cases of uniform and variable coefficient(s). Different choices for the 1/T scaling appearing in the eddy-viscosity were also evaluated. It was found that the models for the vorticity-velocity formulation produce higher correlations with the filtered DNS data than their counterpart in the velocity-pressure formulation. It was also found that the hyper eddy-viscosity model performs better than the eddy viscosity model, in both formulations.

Kinetics of the Reactions of IO Radicals with NO and NO2

NASA Technical Reports Server (NTRS)

Daykin, E. P.; Wine, P. H.

1997-01-01

A laser flash photolysis-long path absorption technique has been employed to study the kinetics of the reactions of IO radicals with NO and NO2 as a function of temperature and pressure. The IO and NO rate coefficient is independent of pressure over the range 40-200 Torr of N2, and its temperature dependence over the range 242-359 K is adequately described by the Arrhenius expression k(sub 1) = (6.9 +/- 1.7) x 10(exp -12) exp[(328 +/- 71)/T] cu cm/(molecule.s) (errors are 2 sigma, precision only). These Arrhenius parameters are similar to those determined previously for the ClO + NO and BrO + NO reactions. The IO and NO2 association reaction is found to be in the falloff regime over the temperature and pressure ranges investigated (254-354 K and 40-750 Torr of N2). Assuming F(sub c) = 0.4 independent of temperature, a physically reasonable set of falloff parameters which adequately describe the data are k(sub 0) = 7.7 x 10(exp -31)(T/300)(exp -5.0) cm(exp 6)/(molecule(exp 2).s) and k(sub infinity) = 1.55 x 10(exp -11)cu cm/(molecule.s) independent of temperature. The IO + NO2 rate coefficients determined in this study are about a factor of 2 faster than those reported in the only previous study of this reaction.

A Small Molecule that Targets r(CGG)exp and Improves Defects in Fragile X-Associated Tremor Ataxia Syndrome

PubMed Central

Disney, Matthew D.; Liu, Biao; Yang, Wang-Yong; Sellier, Chantal; Tran, Tuan; Charlet-Berguerand, Nicolas; Childs-Disney, Jessica L.

2012-01-01

The development of small molecule chemical probes or therapeutics that target RNA remains a significant challenge despite the great interest in such compounds. The most significant barrier to compound development is a lack of knowledge of the chemical and RNA motif spaces that interact specifically. Herein, we describe a bioactive small molecule probe that targets expanded r(CGG) repeats, or r(CGG)exp , that causes Fragile X-associated Tremor Ataxia Syndrome (FXTAS). The compound was identified by using information on the chemotypes and RNA motifs that interact. Specifically, 9-hydroxy-5,11-dimethyl-2-(2-(piperidin-1-yl)ethyl)-6H-pyrido[4,3-b]carbazol-2-ium, binds the 5’CGG/3’GGC motifs in r(CGG)exp and disrupts a toxic r(CGG)exp -protein complex in vitro. Structure-activity relationships (SAR) studies determined that the alkylated pyridyl and phenolic side chains are important chemotypes that drive molecular recognition to r(CGG)exp . Importantly, the compound is efficacious in FXTAS model cellular systems as evidenced by its ability to improve FXTAS-associated pre-mRNA splicing defects and to reduce the size and number of r(CGG)exp -protein aggregates. This approach may establish a general strategy to identify lead ligands that target RNA while also providing a chemical probe to dissect the varied mechanisms by which r(CGG)exp promotes toxicity. PMID:22948243

Small Molecule Recognition and Tools to Study Modulation of r(CGG)(exp) in Fragile X-Associated Tremor Ataxia Syndrome.

PubMed

Yang, Wang-Yong; He, Fang; Strack, Rita L; Oh, Seok Yoon; Frazer, Michelle; Jaffrey, Samie R; Todd, Peter K; Disney, Matthew D

2016-09-16

RNA transcripts containing expanded nucleotide repeats cause many incurable diseases via various mechanisms. One such disorder, fragile X-associated tremor ataxia syndrome (FXTAS), is caused by a noncoding r(CGG) repeat expansion (r(CGG)(exp)) that (i) sequesters proteins involved in RNA metabolism in nuclear foci, causing dysregulation of alternative pre-mRNA splicing, and (ii) undergoes repeat associated non-ATG translation (RANT), which produces toxic homopolymeric proteins without using a start codon. Here, we describe the design of two small molecules that inhibit both modes of toxicity and the implementation of various tools to study perturbation of these cellular events. Competitive Chemical Cross Linking and Isolation by Pull Down (C-Chem-CLIP) established that compounds bind r(CGG)(exp) and defined small molecule occupancy of r(CGG)(exp) in cells, the first approach to do so. Using an RNA GFP mimic, r(CGG)(exp)-Spinach2, we observe that our optimal designed compound binds r(CGG)(exp) and affects RNA localization by disrupting preformed RNA foci. These events correlate with an improvement of pre-mRNA splicing defects caused by RNA gain of function. In addition, the compounds reduced levels of toxic homopolymeric proteins formed via RANT. Polysome profiling studies showed that small molecules decreased loading of polysomes onto r(CGG)(exp), explaining decreased translation.

A small molecule that targets r(CGG)(exp) and improves defects in fragile X-associated tremor ataxia syndrome.

PubMed

Disney, Matthew D; Liu, Biao; Yang, Wang-Yong; Sellier, Chantal; Tran, Tuan; Charlet-Berguerand, Nicolas; Childs-Disney, Jessica L

2012-10-19

The development of small molecule chemical probes or therapeutics that target RNA remains a significant challenge despite the great interest in such compounds. The most significant barrier to compound development is defining which chemical and RNA motif spaces interact specifically. Herein, we describe a bioactive small molecule probe that targets expanded r(CGG) repeats, or r(CGG)(exp), that causes Fragile X-associated Tremor Ataxia Syndrome (FXTAS). The compound was identified by using information on the chemotypes and RNA motifs that interact. Specifically, 9-hydroxy-5,11-dimethyl-2-(2-(piperidin-1-yl)ethyl)-6H-pyrido[4,3-b]carbazol-2-ium binds the 5'CGG/3'GGC motifs in r(CGG)(exp) and disrupts a toxic r(CGG)(exp)-protein complex in vitro. Structure-activity relationship studies determined that the alkylated pyridyl and phenolic side chains are important chemotypes that drive molecular recognition of r(CGG)(exp). Importantly, the compound is efficacious in FXTAS model cellular systems as evidenced by its ability to improve FXTAS-associated pre-mRNA splicing defects and to reduce the size and number of r(CGG)(exp)-containing nuclear foci. This approach may establish a general strategy to identify lead ligands that target RNA while also providing a chemical probe to dissect the varied mechanisms by which r(CGG)(exp) promotes toxicity.

Closed form of the Baker-Campbell-Hausdorff formula for the generators of semisimple complex Lie algebras

NASA Astrophysics Data System (ADS)

Matone, Marco

2016-11-01

Recently it has been introduced an algorithm for the Baker-Campbell-Hausdorff (BCH) formula, which extends the Van-Brunt and Visser recent results, leading to new closed forms of BCH formula. More recently, it has been shown that there are 13 types of such commutator algebras. We show, by providing the explicit solutions, that these include the generators of the semisimple complex Lie algebras. More precisely, for any pair, X, Y of the Cartan-Weyl basis, we find W, linear combination of X, Y, such that exp (X) exp (Y)=exp (W). The derivation of such closed forms follows, in part, by using the above mentioned recent results. The complete derivation is provided by considering the structure of the root system. Furthermore, if X, Y, and Z are three generators of the Cartan-Weyl basis, we find, for a wide class of cases, W, a linear combination of X, Y and Z, such that exp (X) exp (Y) exp (Z)=exp (W). It turns out that the relevant commutator algebras are type 1c-i, type 4 and type 5. A key result concerns an iterative application of the algorithm leading to relevant extensions of the cases admitting closed forms of the BCH formula. Here we provide the main steps of such an iteration that will be developed in a forthcoming paper.

Diffusion length damage coefficient and annealing studies in proton-irradiated InP

NASA Technical Reports Server (NTRS)

Hakimzadeh, Roshanak; Vargas-Aburto, Carlos; Bailey, Sheila G.; Williams, Wendell

1993-01-01

We report on the measurement of the diffusion length damage coefficient (K(sub L)) and the annealing characteristics of the minority carrier diffusion length (L(sub n)) in Czochralski-grown zinc-doped indium phosphide (InP), with a carrier concentration of 1 x 10(exp l8) cm(exp -3). In measuring K(sub L) irradiations were made with 0.5 MeV protons with fluences ranging from 1 x 10(exp 11) to 3 x 10(exp 13) cm(exp -2). Pre- and post-irradiation electron-beam induced current (EBIC) measurements allowed for the extraction of L(sub n) from which K(sub L) was determined. In studying the annealing characteristics of L(sub n) irradiations were made with 2 MeV protons with fluence of 5 x 10(exp 13) cm(exp -2). Post-irradiation studies of L(sub n) with time at room temperature, and with minority carrier photoinjection and forward-bias injection were carried out. The results showed that recovery under Air Mass Zero (AMO) photoinjection was complete. L(sub n) was also found to recover under forward-bias injection, where recovery was found to depend on the value of the injection current. However, no recovery of L(sub n) after proton irradiation was observed with time at room temperature, in contrast to the behavior of 1 MeV electron-irradiated InP solar cells reported previously.

Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-β-catenin axis

PubMed Central

Wang, Hong; Fan, Huimin; Li, Yan; Wang, Jianing; Zhang, Xu; Lu, Jinrong; Ji, Hui; Hu, Rong

2016-01-01

Nonresolving inflammation in the intestine predisposes individuals to colitis-associated colorectal cancer (CAC), which leads to high morbidity and mortality. Here we show that genistein-27 (GEN-27), a derivative of genistein, inhibited proliferation of human colorectal cancer cells through inhibiting β-catenin activity. Our results showed that GEN-27 increased expressions of adenomatous polyposis coli (APC) and axis inhibition protein 2 (AXIN2), and reduced β-catenin nuclear localization, which resulted from the inhibition of NF-κB/p65 nuclear localization and up-regulation of caudal-related homeobox transcription factor 2 (CDX2). Furthermore, GEN-27 decreased binding of p65 to the silencer region of CDX2 and increased binding of CDX2 to the promoter regions of APC and AXIN2, thus inhibiting the activation of β-catenin induced by TNF-α. Importantly, GEN-27 protected mice from azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon carcinogenesis, with reduced mortality, tumor number and tumor volume. Histopathology, immunohistochemistry and flow cytometry revealed that dietary GEN-27 significantly decreased secretion of proinflammatory cytokines and macrophage infiltration. Moreover, GEN-27 inhibited AOM/DSS-induced p65 and β-catenin nuclear translocation, while promoted the expression of CDX2, APC, and AXIN2. Taken together, our findings demonstrate that the anti-proliferation effect of GEN-27 in vitro and the prevention of CAC in vivo is mediated by p65-CDX2-β-catenin axis via inhibiting β-catenin target genes. Our results imply that GEN-27 could be a promising candidate for the chemoprevention of CAC. PMID:26910375

Efficacy and Safety of Ixekizumab in a Randomized, Double-Blinded, Placebo-Controlled Phase 3b Study of Patients with Moderate-to-Severe Genital Psoriasis.

PubMed

Ryan, C; Menter, A; Guenther, L; Blauvelt, A; Bissonnette, R; Meeuwis, K; Sullivan, J; Cather, J C; Yosipovitch, G; Gottlieb, A B; Merola, J F; Callis Duffin, K; Fretzin, S; Osuntokun, O O; Burge, R; Naegeli, A N; Yang, F E; Lin, C-Y; Todd, K; Potts Bleakman, A

2018-05-10

Genital psoriasis (GenPs) is a common, debilitating, and difficult to treat manifestation of plaque psoriasis. However, few controlled, interventional studies of GenPs exist. To determine the efficacy of ixekizumab versus placebo in patients with moderate-to-severe GenPs with BSA≥1%. Subjects with moderate-to-severe GenPs (defined as a baseline static Physician's Global Assessment of Genitalia [sPGA-G] score of ≥3) with BSA≥1% were randomized 1:1 to receive placebo (N=74) or the recommended dosing of ixekizumab (N=75). Major outcomes included the percentage of patients achieving 0 or 1 scores on the sPGA-G (primary endpoint), overall sPGA, GenPs Sexual Frequency Questionnaire (GenPs-SFQ) item 2, and a ≥3-point improvement from baseline on the GenPs itch numeric rating scale. At week 12, ixekizumab was superior to placebo on the sPGA-G 0/1 (73.3% versus 8.1%, p<0.001), overall sPGA 0/1 (73.3% versus 2.7%, p<0.001), GenPs-SFQ item 2 0/1 (78.4% versus 21.4%, p<0.001), and genital itch (59.7% versus 8.3%, p<0.001). No candidiasis was reported, no deaths occurred, and one (1.4%) serious adverse event was reported in a patient receiving placebo. Ixekizumab was superior to placebo for the treatment of moderate-to-severe GenPs with BSA≥1%. The safety profile of ixekizumab was consistent with previous studies in moderate-to-severe plaque psoriasis. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Effects of Chronic Genistein Treatment in Mammary Gland, Uterus, and Vagina

PubMed Central

Rimoldi, Guillermo; Christoffel, Julie; Seidlova-Wuttke, Dana; Jarry, Hubertus; Wuttke, Wolfgang

2007-01-01

Background The isoflavone genistein (GEN) is found in soy (Glycine max) and red clover (Trifolium pratense). The estrogenic activity of GEN is known, and it is widely advertised as a phytoestrogen useful in alleviating climacteric complaints and other postmenopausal disorders. Knowledge of effects of long-term administration of GEN in laboratory animals is scarce, and effects in the uterus and mammary gland after long-term administration have not been studied. The uterus and mammary gland are known to be negatively influenced by estrogens used in hormone therapy. Objectives We administered two doses of GEN [mean daily uptake 5.4 (low) or 54 mg/kg (high) body weight (bw)] orally over a period of 3 months to ovariectomized (ovx) rats and compared the effects with a treatment with two doses of 17β-estradiol [E2; 0.17 (low) or 0.7 mg/kg bw (high)]. Mammary glands, vaginae, and uteri were investigated morphologically and immunohistochemically. We quantified the expression of proliferating cell nuclear antigen (PCNA) and progesterone receptor (PR) in the mammary gland. Results In rats treated with either of the E2 doses or the high GEN dose, we found increased uterine weight, and histologic analysis showed estrogen-induced features in the uteri. In vaginae, either E2 dose or GEN high induced hyperplastic epithelium compared with the atrophic controls. In the mammary gland, E2 (either dose) or GEN increased proliferation and PR expression. Serum levels of luteinizing hormone were decreased by E2 (both doses) but not by GEN. Conclusions In summary, E2 and GEN share many effects in the studied organs, particularly in the vagina, uterus, and mammary gland but not in the hypothalamo/pituitary unit. PMID:18174952

Foreign Trip Report MATGEN-IV Sep 24- Oct 26, 2007

DOE Office of Scientific and Technical Information (OSTI.GOV)

de Caro, M S

2007-10-30

Gen-IV activities in France, Japan and US focus on the development of new structural materials for Gen-IV nuclear reactors. Oxide dispersion strengthened (ODS) F/M steels have raised considerable interest in nuclear applications. Promising collaborations can be established seeking fundamental knowledge of relevant Gen-IV ODS steel properties (see attached travel report on MATGEN- IV 'Materials for Generation IV Nuclear Reactors'). Major highlights refer to results on future Ferritic/Martensitic steel cladding candidates (relevant to Gen-IV materials properties for LFR Materials Program) and on thermodynamic and mechanic behavior of metallic FeCr binary alloys, base matrix for future candidate steels (for the LLNL-LDRD projectmore » on Critical Issues on Materials for Gen-IV Reactors).« less

Discovering the Theorem of Pythagoras

NASA Technical Reports Server (NTRS)

Lattanzio, Robert (Editor)

1988-01-01

In this 'Project Mathematics! series, sponsored by the California Institute of Technology, Pythagoraus' theorem a(exp 2) + b(exp 2) = c(exp 2) is discussed and the history behind this theorem is explained. hrough live film footage and computer animation, applications in real life are presented and the significance of and uses for this theorem are put into practice.

75 FR 56654 - RTCA NextGen Advisory Committee (NAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-16

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration RTCA NextGen Advisory Committee (NAC) AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA NextGen Advisory Committee (NAC) SUMMARY: The FAA published a Notice in the Federal Register on September 3, 2010 (75-FR-54221...

Effects of reduced digestible lysine density on myopathies of the Pectoralis major muscles in broiler chickens at 48 and 62 days of age.

PubMed

Meloche, K J; Fancher, B I; Emmerson, D A; Bilgili, S F; Dozier, W A

2018-05-14

Quantitative control of nutrient intake may decrease the incidence of wooden breast (WB) and white striping (WS) myopathies with some impairment of live performance. Two experiments (Exp) utilizing Yield Plus × Ross 708 male broilers were conducted to determine if a reduction in myopathies may be obtained through a qualitative approach by reducing digestible lysine (dLys) density. All birds received an identical starter diet until 11 d of age. In Exp 1 (63 pens; 22 birds/pen), each pen was then randomly assigned to 1 of the following 7 dietary treatments (TRT) for a 47 d production period. Seven dietary treatments were provided for the grower 1 (G1; 12 to 18 d of age) and grower 2 (G2; 19 to 26 d of age) phases: 1) 100% of primary breeder recommendations for dLys throughout Exp; 2) 85% of TRT 1 dLys for G1; 3) 85% of TRT 1 dLys for G2; 4) 85% of TRT 1 dLys for G1 and G2; 5) 75% of TRT 1 dLys for G1; 6) 75% of TRT 1 dLys for G2; 7) 75% of TRT 1 for G1 and G2. In Exp 2 (24 pens; 30 birds/pen), birds were randomly assigned to 1 of the following 4 dietary TRT (Table 2) during a 61 d production period. Four dietary treatments were provided for the grower (G; 12 to 28 d) and finisher 1 (F1; 29 to 40 d of age) phases: 1) 100% of primary breeder recommendations for dLys 2) 85% of TRT 1 dLys for G; 3) 85% of TRT 1 dLys for F1; 4) 85% of TRT 1 dLys for G and F1; thereafter, birds received common finisher 1 (Exp 1: 27 to 42 d of age), finisher 2 (Exp 2: 41 to 48 d of age) and withdrawal (Exp 1: 43 to 47 d of age; Exp 2: 49 to 61 d of age) diets. Ideal amino acid ratios were not maintained in reduced dLys diets in either Exp. At 48 (Exp 1; 18 birds/pen) and 62 (Exp 2; 30 birds/pen) d of age, selected birds were processed and fillets were visually scored for WB and WS. No differences (P > 0.05) in cumulative live performance responses between TRT 1 and the remaining TRT were observed in either Exp. In Exp 1, the incidence of severe WB (20.8%) and WS (42.3%) at 48 d of age among birds receiving TRT 7 was reduced (P < 0.01) compared with TRT 1 (WB: 36.6%; WS: 64.3%), at the expense of reduced (P = 0.003) breast weights and yield. In Exp 2, the incidence of severe WB (18.8%) and WS (17.8%) at 62 d of age for birds receiving TRT 4 was reduced (P < 0.05) compared with TRT 1 (WB: 39.3%; WS: 38.3%), without any detrimental effects on processing characteristics. These results indicate that altering dietary dLys during critical periods of the growth trajectory may be a viable strategy for reducing the incidence and severity of WB and WS.

Local corticosteroid injection in iliotibial band friction syndrome in runners: a randomised controlled trial

PubMed Central

Gunter, P; Schwellnus, M; Fuller, P

2004-01-01

Objective: To establish whether a local injection of methylprednisolone acetate (40 mg) is effective in decreasing pain during running in runners with recent onset (less than two weeks) iliotibial band friction syndrome (ITBFS). Methods: Eighteen runners with at least grade 2 ITBFS underwent baseline investigations including a treadmill running test during which pain was recorded on a visual analogue scale every minute. The runners were then randomly assigned to either the experimental (EXP; nine) or a placebo control (CON; nine) group. The EXP group was infiltrated in the area where the iliotibial band crosses the lateral femoral condyle with 40 mg methylprednisolone acetate mixed with a short acting local anaesthetic, and the CON group with short acting local anaesthetic only. The same laboratory based running test was repeated after seven and 14 days. The main measure of outcome was total pain during running (calculated as the area under the pain versus time graph for each running test). Results: There was a tendency (p = 0.07) for a greater decrease in total pain (mean (SEM)) during the treadmill running in the EXP group than the CON group tests from day 0 (EXP = 222 (71), CON = 197 (31)) to day 7 (EXP = 140 (87), CON = 178 (76)), but there was a significant decrease in total pain during running (p = 0.01) from day 7 (EXP = 140 (87), CON = 178 (76)) to day 14 (EXP = 103 (89), CON = 157 (109)) in the EXP group compared with the CON group. Conclusion: Local corticosteroid infiltration effectively decreases pain during running in the first two weeks of treatment in patients with recent onset ITBFS. PMID:15155424

Kinetics of the Reactions of O((sup 3)P) and Cl((sup 2)P) with HBr and Br2

NASA Technical Reports Server (NTRS)

Nicovich, J. M.; Wine, P. H.

1997-01-01

A laser flash photolysis-resonance fluorescence technique has been employed to study the kinetics of reactions (1)-(4) as a function of temperature. (1) O((sup 3)P) + Br2 yields BrO + Br((sup 2)P(sub 3/2)) at 255-350 K; (2) Cl((sup 2)P) + Br2 yields BrCl + Br((sup 2)P(sub 3/2)) at 298-401 K; (3) O((sup 3)P) + HBr yields OH + Br((sup 2)P(sub J)) at 250-402 K; (4) Cl((sup 2)P) + HBr yields HCl + Br((sup 2)P(sub J)) at 257-404 K. In all cases, the concentration of the excess reagent, i.e, HBr or Br2, was measured in situ in the slow flow system by UV-visible photometry. Heterogeneous dark reactions between XBr (X equals H or Br) and the photolytic precursors for Cl((sup 2)P) and O((sup 3)P) (Cl2 and O3, respectively) were avoided by injecting minimal amounts of precursor into the reaction mixture immediately upstream from the reaction zone. The following Arrhenius expressions summarize our results (errors are 2 sigma and represent precision only, units are cu cm/(molecule.s): k(sub 1) = (1.76 +/- 0.80) x 10(exp -11 exp[(40 +/- 100)/T]; k(sub 2) = (2.40 +/- 1.25) x 12(exp -10) exp[-(144 +/- 176)/T]; k(sub 3) = (5.11 +/- 2.82) x 10(exp -12) exp[-(1450 +/- 160)/T]; k(sub 4) = (2.25 +/- 0.56) x 10(exp -11) exp[-(400 +/- 80)/T]. The consistency (or lack thereof) of our results with those reported in previous kinetics and dynamics studies of reactions (1)-(4) is discussed.

Resveratrol Improves the Mitochondrial Function and Fertilization Outcome of Bovine Oocytes

PubMed Central

TAKEO, Shun; SATO, Daichi; KIMURA, Koji; MONJI, Yasunori; KUWAYAMA, Takehito; KAWAHARA-MIKI, Ryoka; IWATA, Hisataka

2013-01-01

The aim of the present study was to address the effect of resveratrol-mediated upregulation of sirtuin 1 (SIRT1) during oocyte maturation on mitochondrial function, the developmental ability of oocytes and on mechanisms responsible for blockage of polyspermic fertilization. Oocytes collected from slaughterhouse-derived ovaries were cultured in TCM-199 medium supplemented with 10% FCS and 0 or 20 µM resveratrol (Res). We examined the effect of Res on SIRT1 expression in in vitro-matured oocytes (Exp 1); fertilization and developmental ability (Exp 2); mitochondrial DNA copy number (Mt number), ATP content and mitochondrial membrane potential in matured oocytes (Exp 3); and the time required for proteinase to dissolve the zona pellucida following in vitro fertilization (as a marker of zona pellucida hardening), as well as on the distribution of cortical granules before and after fertilization (Exp 4). In Exp 1, the 20 µM Res treatment upregulated protein expression of SIRT1 in oocytes. In Exp 2, Res treatment improved the ratio of normal fertilization and the total cell number of blastocysts. In Exp 3, Res treatment significantly increased the ATP content in matured oocytes. Additionally, Res increased the overall Mt number and mitochondrial membrane potential, but the effect was donor-dependent. In Exp 4, Res-induced zona hardening improved the distribution and exocytosis of cortical granules after in vitro fertilization. In conclusion, Res improved the quality of oocytes by improving mitochondrial quantity and quality. In addition, Res added to the maturation medium enhanced SIRT1 protein expression in oocytes and improved fertilization via reinforcement of the mechanisms responsible for blockage of polyspermic fertilization. PMID:24390595

The effect of additional carbohydrate supplements for 7 days after prolonged interval exercise on exercise performance and energy metabolism during submaximal exercise in team-sports athletes

PubMed Central

Park, Hun-Young; Kim, Jisu; Park, Miyoung; Chung, Nana; Lim, Kiwon

2018-01-01

[Purpose] The purpose of our study was to determine the effectiveness of carbohydrate loading by additional carbohydrate supplements for 7 days after prolonged interval exercise on exercise performance and energy metabolism during submaximal exercise in team-sports athletes. [Methods] Twenty male team-sports athletes (14 soccer and 6 rugby players) volunteered to participate in the study and were equally divided into the experimental group (EXP, n=10) performing additional carbohydrate supplementation for 7 days after prolonged interval exercise until blood glucose level reaches 50 mg/dL or less and the control group (CON, n=10). Then, maximal oxygen consumption (VO2max) and minute ventilation (VE), oxygen consumption (VO2), carbon dioxide excretion (VCO2), respiratory exchange ratio (RER), blood glucose level, and blood lactate level were measured in all team-sports players during submaximal exercise corresponding to 70% VO2max before and after intervention. [Results] There was no significant interaction in all parameters, but team-sports players in the EXP presented more improved VO2max (CON vs EXP = vs 5.3% vs 6.3%), VE (CON vs EXP = vs 3.8% vs 6.6%), VO2 (CON vs EXP = vs 8.5% vs 9.9%), VCO2 (CON vs EXP = vs 2.8% vs 4.0%), blood glucose level (CON vs EXP = vs -12.9% vs -7.6%), and blood lactate level (CON vs EXP = -18.2% vs -25%) compared to those in the CON. [Conclusion] These findings showed that additional carbohydrate supplementation conducted in our study is not effective in exercise performance and energy metabolism during submaximal exercise. PMID:29673243

Environmental Perturbations Caused by the Impacts of Asteroids and Comets

NASA Technical Reports Server (NTRS)

Toon, Owen B.; Zahnle, Kevin; Morrison, David; Turco, Richard; Covey, Curt

1997-01-01

We review the major mechanisms proposed to cause extinctions at the Cretaceous-Tertiary geological boundary following an asteroid impact. We then discuss how the proposed extinction may relate to the impact of asteroids or comets in general. We discuss the limitations of these mechanisms in terms of the spatial scale that may be affected, and the time scale over which the effects may last. Our goal is to provide relatively simple prescriptions for evaluating the importance of colliding objects having a range of energies and compositions. We also identify the many uncertainties concerning the environmental effects of impacts. We conclude that, for impact energies below about 10(exp 4) Mts (megatons of TNT equivalent) - i.e., impact frequencies less than in 6 x 10(exp 4) yr, corresponding to comets and asteroids with diameters smaller than about 400 m and 650 m, respectively - blast damage, earthquakes, and fires should be important on a scale of 10(exp 4) or 10(exp 5) km (exp 2), which corresponds to the area damaged in many natural disasters of recent history. However, tsunami could be more damaging, flooding a kilometer of coastal plane over entire ocean basins. In the energy range of 10(exp 4) to 10 (exp 5) Mts (intervals up to 3 x 10(exp 5) yr; comets and asteroids with sizes up to 800 m and 1.5 km, respectively) water vapor injections and ozone loss become significant on the global scale. In the submicrometer dust injection fraction from the pulverized target material is much higher than is presently thought to be most likely, then dust injection could be important in this energy range.

Rate Coefficients for Reactions of Ethynyl Radical (C2H) With HCN and CH3CN: Implications for the Formation of Comples Nitriles on Titan

NASA Technical Reports Server (NTRS)

Hoobler, Ray J.; Leone, Stephen R.

1997-01-01

Rate coefficients for the reactions of C2H + HCN yields products and C2H + CH3CN yields products have been measured over the temperature range 262-360 K. These experiments represent an ongoing effort to accurately measure reaction rate coefficients of the ethynyl radical, C2H, relevant to planetary atmospheres such as those of Jupiter and Saturn and its satellite Titan. Laser photolysis of C2H2 is used to produce C2H, and transient infrared laser absorption is employed to measure the decay of C2H to obtain the subsequent reaction rates in a transverse flow cell. Rate constants for the reaction C2H + HCN yields products are found to increase significantly with increasing temperature and are measured to be (3.9-6.2) x 10(exp 13) cm(exp 3) molecules(exp -1) s(exp -1) over the temperature range of 297-360 K. The rate constants for the reaction C2H + CH3CN yields products are also found to increase substantially with increasing temperature and are measured to be (1.0-2.1) x 10(exp -12) cm(exp 3) molecules(exp -1) s(exp -1) over the temperature range of 262-360 K. For the reaction C2H + HCN yields products, ab initio calculations of transition state structures are used to infer that the major products form via an addition/elimination pathway. The measured rate constants for the reaction of C2H + HCN yields products are significantly smaller than values currently employed in photochemical models of Titan, which will affect the HC3N distribution.

Simultaneous transgenic suppression of LePG and LeExp1 influences fruit texture and juice viscosity in a fresh market tomato variety.

PubMed

Powell, Ann L T; Kalamaki, Mary S; Kurien, Philip A; Gurrieri, Sergio; Bennett, Alan B

2003-12-03

Tomatoes are grown for fresh consumption or for processing of the fruit. Some ripening-associated processes of the fruit can either contribute to or degrade attributes associated with both fresh and processing quality. For example, cell wall disassembly is associated with loss of fresh fruit firmness as well as with loss of processed tomato product viscosity. Several enzymes contribute to cell wall polysaccharide disassembly. Polygalacturonase (PG, poly[1,4-alpha-d-galactouronide] glucanohydrolase, EC 3.2.1.15) is among the most abundant polysaccharide hydrolases in ripening tomato fruit and is the major contributor to pectin depolymerization. Expansin (LeExp1) is also abundant in ripening fruit and is proposed to contribute to cell wall disassembly by nonhydrolytic activity, possibly by increasing substrate accessibility to other enzymes. Suppression of either LePG or LeExp1 expression alone results in altered softening and/or shelf life characteristics. To test whether simultaneous suppression of both LePG and LeExp1 expression influences fruit texture in additive or synergistic ways, transgenic Lycopersicon esculentum var. Ailsa Craig lines with reduced expression of either LePG or LeExp1 were crossed. Fruits from the third generation of progeny, homozygous for both transgenic constructs, were analyzed for firmness and other quality traits during ripening on or off the vine. In field-grown transgenic tomato fruit, suppression of LeExp1 or LePG alone did not significantly increase fruit firmness. However, fruits suppressed for both LePG and LeExp1 expression were significantly firmer throughout ripening and were less susceptible to deterioration during long-term storage. Juice prepared from the transgenic tomato fruit with reduced LePG and LeExp1 expression was more viscous than juice prepared from control fruit.

The effect of additional carbohydrate supplements for 7 days after prolonged interval exercise on exercise performance and energy metabolism during submaximal exercise in team-sports athletes.

PubMed

Park, Hun-Young; Kim, Jisu; Park, Miyoung; Chung, Nana; Lim, Kiwon

2018-03-30

The purpose of our study was to determine the effectiveness of carbohydrate loading by additional carbohydrate supplements for 7 days after prolonged interval exercise on exercise performance and energy metabolism during submaximal exercise in team-sports athletes. Twenty male team-sports athletes (14 soccer and 6 rugby players) volunteered to participate in the study and were equally divided into the experimental group (EXP, n=10) performing additional carbohydrate supplementation for 7 days after prolonged interval exercise until blood glucose level reaches 50 mg/dL or less and the control group (CON, n=10). Then, maximal oxygen consumption (VO2max) and minute ventilation (VE), oxygen consumption (VO2), carbon dioxide excretion (VCO2), respiratory exchange ratio (RER), blood glucose level, and blood lactate level were measured in all team-sports players during submaximal exercise corresponding to 70% VO2max before and after intervention. There was no significant interaction in all parameters, but team-sports players in the EXP presented more improved VO2max (CON vs EXP = vs 5.3% vs 6.3%), VE (CON vs EXP = vs 3.8% vs 6.6%), VO2 (CON vs EXP = vs 8.5% vs 9.9%), VCO2 (CON vs EXP = vs 2.8% vs 4.0%), blood glucose level (CON vs EXP = vs -12.9% vs -7.6%), and blood lactate level (CON vs EXP = -18.2% vs -25%) compared to those in the CON. These findings showed that additional carbohydrate supplementation conducted in our study is not effective in exercise performance and energy metabolism during submaximal exercise. ©2018 The Korean Society for Exercise Nutrition.

Fungal Planet description sheets: 625-715.

PubMed

Crous, P W; Wingfield, M J; Burgess, T I; Carnegie, A J; Hardy, G E St J; Smith, D; Summerell, B A; Cano-Lira, J F; Guarro, J; Houbraken, J; Lombard, L; Martín, M P; Sandoval-Denis, M; Alexandrova, A V; Barnes, C W; Baseia, I G; Bezerra, J D P; Guarnaccia, V; May, T W; Hernández-Restrepo, M; Stchigel, A M; Miller, A N; Ordoñez, M E; Abreu, V P; Accioly, T; Agnello, C; Agustin Colmán, A; Albuquerque, C C; Alfredo, D S; Alvarado, P; Araújo-Magalhães, G R; Arauzo, S; Atkinson, T; Barili, A; Barreto, R W; Bezerra, J L; Cabral, T S; Camello Rodríguez, F; Cruz, R H S F; Daniëls, P P; da Silva, B D B; de Almeida, D A C; de Carvalho Júnior, A A; Decock, C A; Delgat, L; Denman, S; Dimitrov, R A; Edwards, J; Fedosova, A G; Ferreira, R J; Firmino, A L; Flores, J A; García, D; Gené, J; Giraldo, A; Góis, J S; Gomes, A A M; Gonçalves, C M; Gouliamova, D E; Groenewald, M; Guéorguiev, B V; Guevara-Suarez, M; Gusmão, L F P; Hosaka, K; Hubka, V; Huhndorf, S M; Jadan, M; Jurjević, Ž; Kraak, B; Kučera, V; Kumar, T K A; Kušan, I; Lacerda, S R; Lamlertthon, S; Lisboa, W S; Loizides, M; Luangsa-Ard, J J; Lysková, P; Mac Cormack, W P; Macedo, D M; Machado, A R; Malysheva, E F; Marinho, P; Matočec, N; Meijer, M; Mešić, A; Mongkolsamrit, S; Moreira, K A; Morozova, O V; Nair, K U; Nakamura, N; Noisripoom, W; Olariaga, I; Oliveira, R J V; Paiva, L M; Pawar, P; Pereira, O L; Peterson, S W; Prieto, M; Rodríguez-Andrade, E; Rojo De Blas, C; Roy, M; Santos, E S; Sharma, R; Silva, G A; Souza-Motta, C M; Takeuchi-Kaneko, Y; Tanaka, C; Thakur, A; Smith, M Th; Tkalčec, Z; Valenzuela-Lopez, N; van der Kleij, P; Verbeken, A; Viana, M G; Wang, X W; Groenewald, J Z

2017-12-01

Novel species of fungi described in this study include those from various countries as follows: Antarctica : Cadophora antarctica from soil. Australia : Alfaria dandenongensis on Cyperaceae , Amphosoma persooniae on Persoonia sp., Anungitea nullicana on Eucalyptus sp . , Bagadiella eucalypti on Eucalyptus globulus , Castanediella eucalyptigena on Eucalyptus sp., Cercospora dianellicola on Dianella sp., Cladoriella kinglakensis on Eucalyptus regnans , Cladoriella xanthorrhoeae (incl. Cladoriellaceae fam. nov. and Cladoriellales ord. nov.) on Xanthorrhoea sp., Cochlearomyces eucalypti (incl. Cochlearomyces gen. nov. and Cochlearomycetaceae fam. nov.) on Eucalyptus obliqua , Codinaea lambertiae on Lambertia formosa , Diaporthe obtusifoliae on Acacia obtusifolia , Didymella acaciae on Acacia melanoxylon , Dothidea eucalypti on Eucalyptus dalrympleana , Fitzroyomyces cyperi (incl. Fitzroyomyces gen. nov.) on Cyperaceae , Murramarangomyces corymbiae (incl. Murramarangomyces gen. nov., Murramarangomycetaceae fam. nov. and Murramarangomycetales ord. nov.) on Corymbia maculata , Neoanungitea eucalypti (incl. Neoanungitea gen. nov.) on Eucalyptus obliqua , Neoconiothyrium persooniae (incl. Neoconiothyrium gen. nov.) on Persoonia laurina subsp. laurina , Neocrinula lambertiae (incl. Neocrinulaceae fam. nov.) on Lambertia sp., Ochroconis podocarpi on Podocarpus grayae , Paraphysalospora eucalypti (incl. Paraphysalospora gen. nov.) on Eucalyptus sieberi , Pararamichloridium livistonae (incl. Pararamichloridium gen. nov., Pararamichloridiaceae fam. nov. and Pararamichloridiales ord. nov.) on Livistona sp., Pestalotiopsis dianellae on Dianella sp., Phaeosphaeria gahniae on Gahnia aspera , Phlogicylindrium tereticornis on Eucalyptus tereticornis , Pleopassalora acaciae on Acacia obliquinervia , Pseudodactylaria xanthorrhoeae (incl. Pseudodactylaria gen. nov., Pseudodactylariaceae fam. nov. and Pseudodactylariales ord. nov.) on Xanthorrhoea sp., Pseudosporidesmium lambertiae (incl. Pseudosporidesmiaceae fam. nov.) on Lambertia formosa , Saccharata acaciae on Acacia sp., Saccharata epacridis on Epacris sp., Saccharata hakeigena on Hakea sericea , Seiridium persooniae on Persoonia sp., Semifissispora tooloomensis on Eucalyptus dunnii , Stagonospora lomandrae on Lomandra longifolia , Stagonospora victoriana on Poaceae , Subramaniomyces podocarpi on Podocarpus elatus , Sympoventuria melaleucae on Melaleuca sp . , Sympoventuria regnans on Eucalyptus regnans , Trichomerium eucalypti on Eucalyptus tereticornis , Vermiculariopsiella eucalypticola on Eucalyptus dalrympleana , Verrucoconiothyrium acaciae on Acacia falciformis , Xenopassalora petrophiles (incl. Xenopassalora gen. nov.) on Petrophile sp . , Zasmidium dasypogonis on Dasypogon sp., Zasmidium gahniicola on Gahnia sieberiana. Brazil : Achaetomium lippiae on Lippia gracilis , Cyathus isometricus on decaying wood, Geastrum caririense on soil, Lycoperdon demoulinii (incl. Lycoperdon subg. Arenicola ) on soil, Megatomentella cristata (incl. Megatomentella gen. nov.) on unidentified plant, Mutinus verrucosus on soil, Paraopeba schefflerae (incl. Paraopeba gen. nov.) on Schefflera morototoni , Phyllosticta catimbauensis on Mandevilla catimbauensis , Pseudocercospora angularis on Prunus persica , Pseudophialophora sorghi on Sorghum bicolor , Spumula piptadeniae on Piptadenia paniculata. Bulgaria : Yarrowia parophonii from gut of Parophonus hirsutulus . Croatia : Pyrenopeziza velebitica on Lonicera borbasiana. Cyprus : Peziza halophila on coastal dunes. Czech Republic : Aspergillus contaminans from human fingernail. Ecuador : Cuphophyllus yacurensis on forest soil, Ganoderma podocarpense on fallen tree trunk. England : Pilidium anglicum (incl. Chaetomellales ord. nov.) on Eucalyptus sp. France : Planamyces parisiensis (incl. Planamyces gen. nov.) on wood inside a house. French Guiana : Lactifluus ceraceus on soil. Germany : Talaromyces musae on Musa sp. India : Hyalocladosporiella cannae on Canna indica , Nothophoma raii from soil. Italy : Setophaeosphaeria citri on Citrus reticulata , Yuccamyces citri on Citrus limon. Japan : Glutinomyces brunneus (incl. Glutinomyces gen. nov.) from roots of Quercus sp. Netherlands (all from soil): Collariella hilkhuijsenii , Fusarium petersiae , Gamsia kooimaniorum , Paracremonium binnewijzendii , Phaeoisaria annesophieae , Plectosphaerella niemeijerarum , Striaticonidium deklijnearum , Talaromyces annesophieae , Umbelopsis wiegerinckiae , Vandijckella johannae (incl. Vandijckella gen. nov. and Vandijckellaceae fam. nov.), Verhulstia trisororum (incl. Verhulstia gen. nov.). New Zealand : Lasiosphaeria similisorbina on decorticated wood. Papua New Guinea : Pseudosubramaniomyces gen. nov. (based on Pseudosubramaniomyces fusisaprophyticus comb. nov.). Slovakia : Hemileucoglossum pusillum on soil. South Africa : Tygervalleyomyces podocarpi (incl. Tygervalleyomyces gen. nov.) on Podocarpus falcatus. Spain : Coniella heterospora from herbivorous dung, Hymenochaete macrochloae on Macrochloa tenacissima , Ramaria cistophila on shrubland of Cistus ladanifer. Thailand : Polycephalomyces phaothaiensis on Coleoptera larvae, buried in soil. Uruguay : Penicillium uruguayense from soil. Vietnam : Entoloma nigrovelutinum on forest soil, Volvariella morozovae on wood of unknown tree. Morphological and culture characteristics along with DNA barcodes are provided.

Discovery of X-ray emission associated with the Gum Nebula

NASA Technical Reports Server (NTRS)

Leahy, D. A.; Nousek, J.; Garmire, G.

1992-01-01

The Gum Nebula was observed by the A-2 LED proportional counters on the HEAO-1 satellite as part of the all-sky survey. The first detection of X-ray emission associated with the Gum Nebula is reported. Soft X-ray spectra were constructed from the A-2 LED PHA data. Single temperature Raymond-Smith models were fitted to the observed spectra to yield temperature, column density and emission measure. The temperature is 6 x 10 exp 5 K, the column density 4 x 10 exp 20/sq cm, and the emission measure 5 cm exp-6 pc. The X-ray and optical properties of the Gum Nebula are consistent with a supernova remnant in the shell stage of evolution, which was the product of an energetic (3 x 10 exp 51 ergs) supernova explosion which occurred about 2 x 10 exp 6 yr ago.

Experimental Surface Pressure Data Obtained on 65 deg Delta Wing Across Reynolds Number and Mach Number Ranges. Vol. 3: Medium-radius leading edge

NASA Technical Reports Server (NTRS)

Chu, Julio; Luckring, James M.

1996-01-01

An experimental wind tunnel test of a 65 deg delta wing model with interchangeable leading edges was conducted in the Langley National Transonic Facility (NTF). The objective was to investigate the effects of Reynolds and Mach numbers on slender-wing leading-edge vortex flows with four values of wing leading-edge bluntness. Experimentally obtained pressure data are presented without analysis in tabulated and graphical formats across a Reynolds number range of 6 x 10(exp 6) to 120 x 10(exp 6) at a Mach number of 0.85 and across a Mach number range of 0.4 to 0.9 at Reynolds numbers of 6 x 10(exp 6), 60 x 10(exp 6), and 120 x 10(exp 6). Normal-force and pitching-moment coefficient plots for these Reynolds number and Mach number ranges are also presented.

Evidence for Live Cl-36 in Ca-Al-rich Inclusions from the Ningqiang Carbonaceous Chondrite

NASA Technical Reports Server (NTRS)

Lin, Y.; Guan, Y.; Leshin, L. A.; Ouyang, Z.; Wang, D.

2004-01-01

The short-lived radionuclide Cl-36 decays to either Ar-36 (98.1%, beta(sup -)) or S-36 (1.9%, epsilon and beta(sup +)), with a half life of 3.01 x 10(exp 5) yr. Both the nucleosynthetic and spallation models suggest high initial Cl-36/Cl-35 ratios ((Cl-36/Cl-35)o up to approximately 10(exp -4)) in the early solar system. Previous observed excess Ar-36 in Efremovka matrix has been interpreted to represent a much lower (Cl-36/Cl-35)o ratio of approximately 1 x 10(exp -6). From the observed S-36 excesses in sodalite in calcium aluminum-rich inclusions (CAIs), we report in this study the first direct evidence of the presence of Cl-36 in primitive meteorites. The inferred (Cl-36/Cl-35)o ratios range from approximately 5 x 10(exp -6) to approximately 1 x 10(exp -5).

Space Environmental Effects on Thermal Control Coatings

NASA Technical Reports Server (NTRS)

OBrien, Susan K.; Workman, Gary L.

1997-01-01

The study of long term near ultra-violet (NUV) effects in a vacuum atmosphere, is a crucial element for space applications. NUV radiation causes significant changes in the reflectance of many coatings and types of materials. An ultra high vacuum NUV system was assembled in order to investigate various coatings and materials in this hostile environment. The vacuum is an ion pump that maintains a minimum vacuum in the mid 10(exp -9) range. The system has a base pressure of 10(exp -9) torr and this base pressure is maintained with the ion pump. The NUV exposure was maintained at 2-3 suns which allows accelerated NUV exposure without overheating the samples. The goal of this test was to maintain an intensity of 3.4 x 10(exp -2) Watts/cm(exp 2) which equals 2.9 NUV suns. An NUV sun is defined as 1.16 Watts/cm(exp 2) integrated over wavelength of 200-400 nanometers.

InGaAs/InP solar cells for space application

NASA Technical Reports Server (NTRS)

Karlina, L. B.; Kazantsev, A. B.; Kozlovskii, V. V.; Mokina, I. A.; Shvarts, M. Z.

1995-01-01

The effects of irradiation of In(0.53)Ga(0.47)As/InP (InGaAs/InP) solar cells illuminated through a transparent InP substrate with 1 MeV electrons were measured. These solar cells were developed for bottom cells in tandem solar photovoltaic cell structures. Some InGaAs/InP heterostructures with four layers were grown by liquid phase epitaxy. The structure of the solar cells allowed lightly doped materials in n and p photoactive layers to be used. The base dopant levels ranged from 1.10(exp 17) to 5.10(exp 17) cm(exp -3). The open circuit voltage and the short circuit current were moderately degraded after irradiation with 10(exp 16) cm(exp-2) 1 MeV electrons. This behavior is explained in terms of the device structure and the n and p layer thicknesses.

Quasar evolution and the growth of black holes

NASA Technical Reports Server (NTRS)

Small, Todd A.; Blandford, Roger D.

1992-01-01

A 'minimalist' model of AGN evolution is analyzed that links the measured luminosity function to an elementary description of black hole accretion. The observed luminosity function of bright AGN is extrapolated and simple prescriptions for the growth and luminosity of black holes are introduced to infer quasar birth rates, mean fueling rates, and relict black hole distribution functions. It is deduced that the mean accretion rate scales as (M exp -1./5)(t exp -6.7) and that, for the most conservative model used, the number of relict black holes per decade declines only as M exp -0.4 for black hole masses between 3 x 10 exp 7 and 3 x 10 exp 9 solar masses. If all sufficiently massive galaxies pass through a quasar phase with asymptotic black hole mass a monotonic function of the galaxy mass, then it is possible to compare the space density of galaxies with estimated central masses to that of distant quasars.

A new genus and three new species of miniaturized microhylid frogs from Indochina (Amphibia: Anura: Microhylidae: Asterophryinae)

PubMed Central

Poyarkov, Nikolay A.; Suwannapoom, Chatmongkon; Pawangkhanant, Parinya; Aksornneam, Akrachai; Duong, Tang Van; Korost, Dmitriy V.; Che, Jing

2018-01-01

We report on the discovery of a new genus of microhylid subfamily Asterophryinae from northern and eastern Indochina, containing three new species. Vietnamophryne Gen. nov. are secretive miniaturized frogs (SVL<21 mm) with a mostly semi-fossorial lifestyle. To assess phylogenetic relationships, we studied 12S rRNA – 16S rRNA mtDNA fragments with a final alignment of 2 591 bp for 53 microhylid species. Morphological and osteological characters were analyzed using micro-CT scanning and used to describe the new genus. Results of phylogenetic analyses assigned the new genus into the mainly Australasian subfamily Asterophryinae as a sister taxon to the genus Siamophryne from southern Indochina. The three specimens collected from Gia Lai Province in central Vietnam, Cao Bang Province in northern Vietnam, and Chiang Rai Province in northern Thailand proved to be separate species, different both in morphology and genetics (genetic divergence 3.1%≤P≤5.1%). Our work provides further evidence for the “out of Indo-Eurasia” scenario for Asterophryinae, indicating that the initial cladogenesis and differentiation of this group of frogs occurred in the Indochina Peninsula. To date, each of the three new species of Vietnamophryne Gen. nov. is known only from a single specimen; thus, their distribution, life history, and conservation status require further study. PMID:29683109

Infrared Measurements of Atmospheric Gases Above Mauna Loa, Hawaii, in February 1987

NASA Technical Reports Server (NTRS)

Rinsland, C. P.; Goldman, A.; Murcray, F. J.; Murcray, F. H.; Blatherwick, R. D.; Murcray, D. G.

1988-01-01

Infrared solar absorption spectra recorded at 0.02/ cm resolution from the National Oceanic and Atmospheric Administration (NOAA) Geophysical Monitoring for Climate Change (GMCC) program station at Mauna Loa, Hawaii (latitude 19.5 deg N, longitude 155.6 deg W, elevation 3.40 km), in February 1997 have been analyzed to determine simultaneous total vertical column amounts for 13 atmospheric gases. Average tropospheric concentrations of CO2, N2O, CH4, and CHCIF2 and the daytime diurnal variations or the total columns of NO and NO2 have also been inferred. The retrieved total columns (in molecules /sq cm) of the nondiurnally varying gases are 1.6 +/- 0.2 x 10(exp 15) for HCl, 5.9 +/- 1.2 x 10(exp 15) for HNO3, 2.0 +/- 0.2 x 10(exp 21) for H2O16, 4.4 +/- 0.7 x 10(exp 18) for H2O18, 2.7 +/- 0.1 x 10(exp 17) for HDO, 2.3 +/- 0.2 x 10(exp 19) for CH4, 5.0 +/- 0.5 x 10(exp 21) for CO2, 6.7 +/- 0.8 x 10(exp 18) for O3, 4.3 +/- 0.4 x 10(exp 18) for N2O, 1.0 +/- 0.2 x 10(exp 16) for C2H6, and 9.7 +/- 2.5 x 10(exp 14) for CHClF2. We compare the total column measurements of HCl and HNO3 with previously reported ground-based, aircraft, and satellite measurements. The results for HCl are or particular interest because of the expected temporal increase in the concentration of this gas in the stratosphere. However, systematic differences among stratospheric HCl total column measurements from 1978 to 1980 and the absence of observations of free tropospheric HCl above Mauna Loa make it impossible to obtain a reliable estimate of the trend in the total burden of HCl. The measured HNO3 total column is consistent with aircraft measurements from approx. 12 km altitude. The O3 total column deduced from the IR spectra agrees with correlative Mauna Loa Umkehr measurements within the estimated error limits. The column-averaged D/H ratio of water vapor is (68 +/- 9) x- 10(exp -6), which is 0.44 +/- 0.06 times the reference value of 155.76 x 10(exp -6) for standard mean ocean water (SMOW). This large depletion in the D content of water vapor is similar to published measurements of the upper troposphere and lower stratosphere. Average tropospheric concentrations deduced for CO2, N2O, and CH4 are in good agreement with correlative NOAA GMCC surface data, indicating consistency between the measurement techniques for determining tropospheric volume mixing ratios. Results of the present study indicate that Mauna Loa is a favorable site for infrared monitoring of atmospheric gases. The site is particularly favorable for monitoring the tropospheric volume mixing ratios of long-lived gases, since the high altitude of the tropopause reduces corrections required to account for the decrease in volume mixing ratio in the stratosphere.

Cellular logic array for computation of squares

NASA Technical Reports Server (NTRS)

Shamanna, M.; Whitaker, S.; Canaris, J.

1991-01-01

A cellular logic array is described for squaring binary numbers. This array offers a significant increase in speed, with a relatively small hardware overhead. This improvement is a result of novel implementation of the formula (x + y)exp 2 = x(exp 2) + y(exp 2) + 2(x)(y). These results can also be incorporated in the existing arrays achieving considerable hardware reduction.

Lick Saturn-Voyager reference star catalogue (Klemola, Taraji, and Ocampo 1979): Documentation for the machine-readable version

NASA Technical Reports Server (NTRS)

Warren, Wayne H., Jr.

1990-01-01

The machine readable version of the catalog, as it is currently being distributed from the Astronomical Data Center, is described. The catalog contains accurate equatorial coordinates for 4551 stars in a band of sky against which cameras of the Voyager spacecraft were pointed for observations in the region of Saturn during the flyby. All of the reference stars are in the range 12(exp h) 40(exp m) to 14(exp h) 12(exp m) in right ascension (1950) and +02 to -09 degs in declination. Mean errors of the positions are about 0.25 sec.

Feeding selectivity of Calanus finmarchicus in the Trondheimsfjord

NASA Astrophysics Data System (ADS)

Leiknes, Øystein; Striberny, Anja; Tokle, Nils Egil; Olsen, Yngvar; Vadstein, Olav; Sommer, Ulrich

2014-01-01

The feeding selectivity of Calanus finmarchicus was studied by carrying out three incubation experiments; two experiments with natural seawater sampled during spring bloom (Exp. 1) and post-bloom conditions (Exp. 2) and a third experiment with cultured dinoflagellates and ciliates (Exp. 3). In the first two experiments a gradient in ciliate concentration was created to investigate the potential for prey density dependent selective feeding of C. finmarchicus. Results of microplankton counts indicated C. finmarchicus to be omnivorous. Diatoms contributed chiefly to the diet during spring bloom conditions. Despite the high microphytoplankton biomass during the spring bloom (Exp. 1), ciliates were selected positively by C. finmarchicus when the ciliate biomass exceeded 6.5 μg C L- 1. A selection in favor of large conic ciliates such as Laboea sp. and Strombidium conicum was indicated by positive selectivity indices. Ciliates were throughout positively selected by C. finmarchicus during Exp. 2, and selectivity indices indicated a negative selection of diatoms. The results from Exp. 3 showed that C. finmarchicus has the ability to switch from dinoflagellates to ciliates as sole food source, even if the dinoflagellate was offered in surplus. This suggests that other factors, such as nutrition may be of significance for the feeding selectivity of C. finmarchicus.

Discovery of a biomarker and lead small molecules to target r(GGGGCC)-associated defects in c9FTD/ALS.

PubMed

Su, Zhaoming; Zhang, Yongjie; Gendron, Tania F; Bauer, Peter O; Chew, Jeannie; Yang, Wang-Yong; Fostvedt, Erik; Jansen-West, Karen; Belzil, Veronique V; Desaro, Pamela; Johnston, Amelia; Overstreet, Karen; Oh, Seok-Yoon; Todd, Peter K; Berry, James D; Cudkowicz, Merit E; Boeve, Bradley F; Dickson, Dennis; Floeter, Mary Kay; Traynor, Bryan J; Morelli, Claudia; Ratti, Antonia; Silani, Vincenzo; Rademakers, Rosa; Brown, Robert H; Rothstein, Jeffrey D; Boylan, Kevin B; Petrucelli, Leonard; Disney, Matthew D

2014-09-03

A repeat expansion in C9ORF72 causes frontotemporal dementia and amyotrophic lateral sclerosis (c9FTD/ALS). RNA of the expanded repeat (r(GGGGCC)exp) forms nuclear foci or undergoes repeat-associated non-ATG (RAN) translation, producing "c9RAN proteins." Since neutralizing r(GGGGCC)exp could inhibit these potentially toxic events, we sought to identify small-molecule binders of r(GGGGCC)exp. Chemical and enzymatic probing of r(GGGGCC)8 indicate that it adopts a hairpin structure in equilibrium with a quadruplex structure. Using this model, bioactive small molecules targeting r(GGGGCC)exp were designed and found to significantly inhibit RAN translation and foci formation in cultured cells expressing r(GGGGCC)66 and neurons transdifferentiated from fibroblasts of repeat expansion carriers. Finally, we show that poly(GP) c9RAN proteins are specifically detected in c9ALS patient cerebrospinal fluid. Our findings highlight r(GGGGCC)exp-binding small molecules as a possible c9FTD/ALS therapeutic and suggest that c9RAN proteins could potentially serve as a pharmacodynamic biomarker to assess efficacy of therapies that target r(GGGGCC)exp. Copyright © 2014 Elsevier Inc. All rights reserved.

Discovery of a Biomarker and Lead Small Molecules to Target r(GGGGCC)-Associated Defects in c9FTD/ALS

PubMed Central

Su, Zhaoming; Zhang, Yongjie; Gendron, Tania F.; Bauer, Peter O.; Chew, Jeannie; Yang, Wang-Yong; Fostvedt, Erik; Jansen-West, Karen; Belzil, Veronique V.; Desaro, Pamela; Johnston, Amelia; Overstreet, Karen; Oh, Seok-Yoon; Todd, Peter K.; Berry, James D.; Cudkowicz, Merit E.; Boeve, Bradley F.; Dickson, Dennis; Floeter, Mary Kay; Traynor, Bryan J.; Morelli, Claudia; Ratti, Antonia; Silani, Vincenzo; Rademakers, Rosa; Brown, Robert H.; Rothstein, Jeffrey D.; Boylan, Kevin B.; Petrucelli, Leonard; Disney, Matthew D.

2014-01-01

Summary A repeat expansion in C9ORF72 causes frontotemporal dementia and amyotrophic lateral sclerosis (c9FTD/ALS). RNA of the expanded repeat (r(GGGGCC)exp) forms nuclear foci or undergoes repeat-associated non-ATG (RAN) translation producing “c9RAN proteins”. Since neutralizing r(GGGGCC)exp could inhibit these potentially toxic events, we sought to identify small molecule binders of r(GGGGCC)exp. Chemical and enzymatic probing of r(GGGGCC)8 indicate it adopts a hairpin structure in equilibrium with a quadruplex structure. Using this model, bioactive small molecules targeting r(GGGGCC)exp were designed and found to significantly inhibit RAN translation and foci formation in cultured cells expressing r(GGGGCC)66 and neurons trans-differentiated from fibroblasts of repeat expansion carriers. Finally, we show that poly(GP) c9RAN proteins are specifically detected in c9ALS patient cerebrospinal fluid. Our findings highlight r(GGGGCC)exp-binding small molecules as a possible c9FTD/ALS therapeutic, and suggest c9RAN proteins could potentially serve as a pharmacodynamic biomarker to assess efficacy of therapies that target r(GGGGCC)exp. PMID:25132468

A Hot-electron Direct Detector for Radioastronomy

NASA Technical Reports Server (NTRS)

Karasik, Boris S.; McGrath, William R.; LeDuc, Henry G.; Gershenson, Michael E.

1999-01-01

A hot-electron transition-edge superconducting bolometer with adjustable thermal relaxation speed is proposed. The bolometer contacts are made from a superconductor with high critical temperature which blocks the thermal diffusion of hot carriers into the contacts. Thus electron-phonon interaction is the only mechanism for heat removal. The speed of thermal relaxation for hot electrons in a nanometer-size superconducting bolometer with T(sub c) = 100-300 mK is controlled by the elastic electron mean free path l. The relaxation rate behaves as T(sup 4)l at subkelvin temperatures and can be reduced by a factor of 10-100 by decreasing 1. Then an antenna- or wave guide-coupled bolometer with a time constant approx. = 10(exp -3) to 10(exp -4) s will exhibit photon-noise limited performance at millimeter and submillimeter wavelengths. The bolometer will have a figure-of-merit NEPtau = 10(exp -22) - 10(exp -21) W/Hz at 100 mK which is 10(exp 3) to 10(exp 4) times better (ie: smaller) than that of a state-of-the-art bolometer. A tremendous increase in speed and sensitivity will have a significant impact for observational mapping applications.

Temperature-Dependent Rate Constants and Substituent Effects for the Reactions of Hydroxyl Radicals With Three Partially Fluorinated Ethers

NASA Technical Reports Server (NTRS)

Hsu, K.-J.; DeMore, W. B.

1995-01-01

Rate constants and temperature dependencies for the reactions of OH with CF3OCH3 (HFOC-143a), CF2HOCF2H (HFOC-134), and CF3OCF2H (HFOC-125) were studied using a relative rate technique in the temperature range 298-393 K. The following absolute rate constants were derived: HFOC-143a, 1.9E-12 exp(-1555/T); HFOC-134, 1.9E-12 exp(-2006/T); HFOC-125, 4.7E-13 exp(-2095/T). Units are cm(exp 3)molecule(exp -1) s(exp -1). Substituent effects on OH abstraction rate constants are discussed, and it is shown that the CF3O group has an effect on the OH rate constants similar to that of a fluorine atom. The effects are related to changes in the C-H bond energies of the reactants (and thereby the activation energies) rather than changes in the preexponential factors. On the basis of a correlation of rate constants with bond energies, the respective D(C-H) bond strengths in the three ethers are found to be 102, 104, and 106 kcal/mol, with an uncertainty of about 1 kcal/mol.

Superconducting six-axis accelerometer

NASA Technical Reports Server (NTRS)

Paik, H. J.

1990-01-01

A new superconducting accelerometer, capable of measuring both linear and angular accelerations, is under development at the University of Maryland. A single superconducting proof mass is magnetically levitated against gravity or any other proof force. Its relative positions and orientations with respect to the platform are monitored by six superconducting inductance bridges sharing a single amplifier, called the Superconducting Quantum Interference Device (SQUID). The six degrees of freedom, the three linear acceleration components and the three angular acceleration components, of the platform are measured simultaneously. In order to improve the linearity and the dynamic range of the instrument, the demodulated outputs of the SQUID are fed back to appropriate levitation coils so that the proof mass remains at the null position for all six inductance bridges. The expected intrinsic noise of the instrument is 4 x 10(exp -12)m s(exp -2) Hz(exp -1/2) for linear acceleration and 3 x 10(exp -11) rad s(exp -2) Hz(exp -1/2) for angular acceleration in 1-g environment. In 0-g, the linear acceleration sensitivity of the superconducting accelerometer could be improved by two orders of magnitude. The design and the operating principle of a laboratory prototype of the new instrument is discussed.

Determination of the Rate Coefficients of the SO2 plus O plus M yields SO3 plus M Reaction

NASA Technical Reports Server (NTRS)

Hwang, S. M.; Cooke, J. A.; De Witt, K. J.; Rabinowitz, M. J.

2010-01-01

Rate coefficients of the title reaction R(sub 31) (SO2 +O+M yields SO3 +M) and R(sub 56) (SO2 + HO2 yields SO3 +OH), important in the conversion of S(IV) to S(VI),were obtained at T =970-1150 K and rho (sub ave) = 16.2 micro mol/cubic cm behind reflected shock waves by a perturbation method. Shock-heated H2/ O2/Ar mixtures were perturbed by adding small amounts of SO2 (1%, 2%, and 3%) and the OH temporal profiles were then measured using laser absorption spectroscopy. Reaction rate coefficients were elucidated by matching the characteristic reaction times acquired from the individual experimental absorption profiles via simultaneous optimization of k(sub 31) and k(sub 56) values in the reaction modeling (for satisfactory matches to the observed characteristic times, it was necessary to take into account R(sub 56)). In the experimental conditions of this study, R(sub 31) is in the low-pressure limit. The rate coefficient expressions fitted using the combined data of this study and the previous experimental results are k(sub 31,0)/[Ar] = 2.9 10(exp 35) T(exp ?6.0) exp(?4780 K/T ) + 6.1 10(exp 24) T(exp ?3.0) exp(?1980 K/T ) cm(sup 6) mol(exp ?2)/ s at T = 300-2500 K; k(sub 56) = 1.36 10(exp 11) exp(?3420 K/T ) cm(exp 3)/mol/s at T = 970-1150 K. Computer simulations of typical aircraft engine environments, using the reaction mechanism with the above k(sub 31,0) and k(sub 56) expressions, gave the maximum S(IV) to S(VI) conversion yield of ca. 3.5% and 2.5% for the constant density and constant pressure flow condition, respectively. Moreover, maximum conversions occur at rather higher temperatures (?1200 K) than that where the maximum k(sub 31,0) value is located (approximately 800 K). This is because the conversion yield is dependent upon not only the k(sup 31,0) and k(sup 56) values (production flux) but also the availability of H, O, and HO2 in the system (consumption flux).

A vacuum (10(exp -9) Torr) friction apparatus for determining friction and endurance life of MoSx films

NASA Technical Reports Server (NTRS)

Miyoshi, Kazuhisa; Honecy, Frank S.; Abel, Phillip B.; Pepper, Stephen V.; Spalvins, Talivaldis; Wheeler, Donald R.

1992-01-01

The first part of this paper describes an ultrahigh vacuum friction apparatus (tribometer). The tribometer can be used in a ball-on-disk configuration and is specifically designed to measure the friction and endurance life of solid lubricating films such as MoS(x) in vacuum at a pressure of 10 exp -7 Pa. The sliding mode is typically unidirectional at a constant rotating speed. The second part of this paper presents some representative friction and endurance life data for magnetron sputtered MoS(x) films (110 nm thick) deposited on sputter-cleaned 440 C stainless-steel disk substrates, which were slid against a 6-mm-diameter 440 C stainless-steel bearing ball. All experiments were conducted with loads of 0.49 to 3.6 N (average Hertzian contact pressure, 0.33 to 0.69 GPa), at a constant rotating speed of 120 rpm (sliding velocity ranging from 31 to 107 mm/s due to the range of wear track radii involved in the experiments), in a vacuum of 7 x 10 exp -7 Pa and at room temperature. The results indicate that there are similarities in friction behavior of MoS(x) films overs their life cycles regardless of load applied. The coefficient of friction (mu) decreases as load W increases according to mu = kW exp -1/3. The endurance life E of MoS(x) films decreases as the load W increases according to E = KW exp -1.4 for the load range. The load- (or contract-pressure-) dependent endurance life allows us to reduce the time for wear experiments and to accelerate endurance life testing of MoS(x) films. For the magnetron-sputtered MoS(x) films deposited on 440 C stainless-steel disks: the specific wear rate normalized to the load and the number of revolutions was 3 x 10 exp -8 mm exp 3/N-revolution; the specific wear rate normalized to the load and the total sliding distance was 8 x 10 exp -7 mm exp 3/N-m; and the nondimensional wear coefficient of was approximately 5 x 10 exp -6. The values are almost independent of load in the range 0.49 to 3.6 N (average Hertzian contact pressures of 0.33 to 0.69 GPa).

ROSAT detection of diffuse hot gas in the edge-on galaxy NGC 4631

NASA Technical Reports Server (NTRS)

Wang, Q. David; Walterbos, Rene A. M.; Steakley, Michael F.; Norman, Colin A.; Braun, Robert

1994-01-01

ROSAT observation is presented of the edge-on spiral galaxy NGC 4631, a nearby Sc/SBd galaxy best known for its extended radio halo. Because of the low foreground Galactic X-ray-absorbing gas column density, N(sub H) approximately 1.4 x 10(exp 20)cm(exp -2), this observation is sensitive to gas of temperature greater than or equal to a few times 10(exp 5) K. A soft (approximately 0.25 keV) X-ray radiation out to more than 8 kpc above the midplane of the galaxy was detected. The strongest X-ray emission in the halo is above the central disk, a region of about 3 kpc radius which shows high star formation activity. The X-ray emission in the halo is bordered by two extended filaments of radio continuum emission. Diffuse X-ray emission from hot gas in the galaxy's disk was found. The spectrum of the radiation can be characterized by a thermal plasma with a temperature of 3 x 10(exp 6) K and a radiative cooling rate of approximately 8 x 10(exp 39) ergs s(exp -1). This rate is only a few percent of the estimated supernova energy release in the interstellar medium of the galaxy. Analysis of the X-ray spectrum shows evidence for the presence of a cooler (several times 10(exp 5) K) halo gas component that could consume a much larger fraction of the supernova energy. Strong evidence was found for disk/halo interaction. Hot gas apparently blows out from supershells in the galaxy's disk at a rate of approximately 1 solar mass yr(exp -1). This outflow of hot gas drags magnetic field lines up in the halo and forms a magnetized gaseous halo. If the magnetic field lines are still anchored to the disk gas at large disk radii, the outflowing gas may be confined high above the disk by magnetic pressure. A strong X-ray source which coincides spatially with an H I supershell has been identified. However, the source is likely an extremely luminous X-ray binary with L(sub chi)(0.1 - 2 keV) approximately 5 x 10(exp 39) ergs s(exp -1), which makes it a stellar mass black hole candidate.

Aerosol Optical Properties at the Lulin Atmospheric Background Station in Taiwan and the Influences of Long-Range Transport of Air Pollutants

NASA Technical Reports Server (NTRS)

Hsiao, Ta-Chih; Chen, Wei-Nai; Ye, Wei-Cheng; Lin, Neng-Huei; Tsay, Si-Chee; Lin, Tang-Huang; Lee, Chung-Te; Chuang, Ming-Tung; Pantina, Peter; Wang, Sheng-Hsiang

2016-01-01

The Lulin Atmospheric Background Station (LABS, 23.47 deg. N 120.87 deg. E, 2862 m ASL) in Central Taiwan was constructed in 2006 and is the only high-altitude background station in the western Pacific region for studying the influence of continental outflow. In this study, extensive optical properties of aerosols, including the aerosol light scattering coefficient [Sigma(sub s)] and light absorption coefficient [Sigma(sub a)], were collected from 2013 to 2014. The intensive optical properties, including mass scattering efficiency [Sigma(sub s)], mass absorption efficiency [Sigma(sub a)] single scattering albedo (Omega), scattering Angstrom exponent (A), and backscattering fraction (b), were determined and investigated, and the distinct seasonal cycle was observed. The value of [Alpha(sub a)] began to increase in January and reached a maximum in April; the mean in spring was 5.89 m(exp. 2) g(exp. -1) with a standard deviation (SD) of 4.54 m(exp. 2) g(exp. -1) and a 4.48 m(exp. 2) g(exp. -1) interquartile range (IQR: 2.95-7.43 m(exp. 2) g(exp. -1). The trend was similar in [Sigma(sub a)], with a maximum in March and a monthly mean of 0.84 m(exp. 2) g(exp. -1). The peak values of Omega (Mean = 0.92, SD = 0.03, IQR: 0.90 - 0.93) and A (Mean = 2.22, SD = 0.61, IQR: 2.12 = 2.47) occurred in autumn. These annual patterns of optical properties were associated with different long-range transport patterns of air pollutants such as biomass burning (BB) aerosol in spring and potential anthropogenic emissions in autumn. The optical measurements performed at LABS during spring in 2013 were compared with those simultaneously performed at the Doi Ang Kang Meteorology Station, Chiang Mai Province, Thailand (DAK, 19.93 deg. N, 99.05 deg. E, 1536 m a.s.l.), which is located in the Southeast Asia BB source region. Furthermore, the relationships among [Sigma(sub s)], [Sigma(sub a)], and (b) were used to characterize the potential aerosol types transported to LABS during different seasons, and the data were inspected according to the HYSPLIT 5-day backward trajectories, which differentiate between different regions of air mass origin.

Coronal heating by the resonant absorption of Alfven waves: The effect of viscous stress tensor

NASA Technical Reports Server (NTRS)

Ofman, L.; Davila, J. M.; Steinolfson, R. S.

1994-01-01

The time-dependent linearized magnetohydrodynamics (MHD) equations for a fully compressible, low-beta, viscoresistive plasma are solved numerically using an implicit integration scheme. The full viscosity stress tensor (Braginskii 1965) is included with the five parameters eta(sub i) i = 0 to 4. In agreement with previous studies, the numerical simulations demonstrate that the dissipation on inhomogeneities in the background Alfven speed occurs in a narrow resonant layer. For an active region in the solar corona the values of eta(sub i) are eta(sub o) = 0.65 g/cm/s, eta(sub 1) = 3.7 x 10(exp -12) g/cm/s, eta(sub 2) = 4 eta(sub 1), eta(sub 3) = 1.4 x 10(exp -6) g/cm/s, eta(sub 4) = 2 eta(sub 3), with n = 10(exp 10)/cu cm, T = 2 x 10(exp 6) K, and B = 100 G. When the Lundquist number S = 10(exp 4) and R(sub 1) much greater than S (where R(sub 1) is the dimensionless shear viscous number) the width of the resistive dissipation layer d(sub r) is 0.22a (where a is the density gradient length scale) and d(sub r) approximately S(exp -1/3). When S much greater than R(sub 1) the shear viscous dissipation layer width d(sub r) scales as R(sub 1)(exp -1/3). The shear viscous and the resistive dissipation occurs in an overlapping narrow region, and the total heating rate is independent of the value of the dissipation parameters in agreement with previous studies. Consequently, the maximum values of the perpendicular velocity and perpendicular magnetic field scale as R(sub 1)(exp -1/3). It is evident from the simulations that for solar parameters the heating due to the compressive viscosity (R(sub 0) = 560) is negligible compared to the resistive and the shear viscous (R(sub 1)) dissipation and it occurs in a broad layer of order a in width. In the solar corona with S approximately equals 10(exp 4) and R(sub 1) approximately equals 10(exp 14) (as calculated from the Braginskii expressions), the shear viscous resonant heating is of comparable magnitude to the resistive resonant heating.

Precision X-ray Timing of RX J0806.3+1527 with CHANDRA: Evidence for Gravitational Radiation from an Ultracompact Binary

NASA Technical Reports Server (NTRS)

Strohymayer, Tod E.

2004-01-01

RX J0806.3+1527 is a candidate double degenerate binary with possibly the shortest known orbital period. The source shows an approximately equal to 100% X-ray intensity modulation at the putative orbital frequency of 3.11 mHz (321.5 s). If the system is a detached, ultracompact binary gravitational radiation should drive spin-up with a magnitude of nu(sup dot) approximately 10(exp -16) Hz per second. Efforts to constrain the X-ray frequency evolution to date have met with mixed success, principally due to the sparseness of earlier observations. Here we describe the results of the first phase coherent X-ray monitoring campaign on RX J0806.3+1527 with Chandra. We obtained a total of 70 ksec of exposure in 6 epochs logarithmically spaced over 320 days. With these data we conclusively show that the X-ray frequency is increasing at a rate of 3.77 plus or minus 0.8 x 10(exp -16) Hz per second. Using the ephemeris derived from the new data we are able to phase up all the earlier Chandra and ROSAT data and show they are consistent with a constant nu(sup dot) = 3.63 plus or minus 0.06 x 10(exp -16) Hz per second over the past decade. This value appears consistent with that recently derived by Israel et al. largely from monitoring of the optical modulation, and is in rough agreement with the solutions reported initially by Hakala et al., based on ground-based optical observations. The large and stable nu(sup dot) over a decade is consistent with gravitational radiation losses driving the evolution. An intermediate polar (IP) scenario where the observed X-ray period is the spin period of an accreting white dwarf appears less tenable because the observed nu(sup dot) requires an m(sup dot) approximately equal to 4 x 10 (exp -8) solar mass yr(sup -l), that is much larger than that inferred from the observed X-ray luminosity (although this depends on the uncertain distance and bolometric corrections), and it is difficult to drive such a high m(sup dot) in a binary system with parameters consistent with all the multiwavelength data. If the ultracompact scenario is correct, then the X-ray flux cannot be powered by stable accretion which would drive the components apart, suggesting a new type of energy source (perhaps electromagnetic) may power the X-ray flux.

CottonGen: a genomics, genetics and breeding database for cotton research

USDA-ARS?s Scientific Manuscript database

CottonGen (http://www.cottongen.org) is a curated and integrated web-based relational database providing access to publicly available genomic, genetic and breeding data for cotton. CottonGen supercedes CottonDB and the Cotton Marker Database, with enhanced tools for easier data sharing, mining, vis...

Case study of read-across predictions using a Generalized Read-Across (GenRA) Approach (10th World Congress)

EPA Science Inventory

We developed the Generalized Read-Across (GenRA) approach to facilitate automated, algorithmic read across predictions. GenRA uses in vitro bioactivity data in conjunction with chemical information to predict up to 574 different apical outcomes from repeat-dose toxicity studies. ...

Essential Elements for Recruitment and Retention: Generation Y

ERIC Educational Resources Information Center

Luscombe, Jenna; Lewis, Ioni; Biggs, Herbert C.

2013-01-01

Purpose: Generation Y (Gen Y) is the newest and largest generation entering the workforce. Gen Y may differ from previous generations in work-related characteristics which may have recruitment and retention repercussions. Currently, limited theoretically-based research exists regarding Gen Y's work expectations and goals in relation to…

Titanogryllus n. gen., the largest Gryllinae cricket from the Neotropical Region with three new species from the Brazilian Atlantic Forest (Orthoptera, Grylloidea, Gryllidae).

PubMed

Jaiswara, Ranjana; Souza-Dias, Pedro G B; De Campos, Lucas Denadai; RedÜ, Darlan R; De Mello, Francisco de A G; Desutter-Grandcolas, Laure

2018-03-29

Titanogryllus, a new genus and three new species T. salgado n. gen. n. sp., T. oxossi n. gen. n. sp., and T. oxente n. gen. n. sp. from subfamily Gryllinae (Grylloidea, Gryllidae) are described from the Brazilian Atlantic Forest. This genus is characterized by its very large size, and establishes a new record for the largest known cricket from Neotropical Region. The new taxa are characterized by their external morphology and male and female genitalia.

Expansion of the 'Reticulosphere': Diversity of Novel Branching and Network-forming Amoebae Helps to Define Variosea (Amoebozoa).

PubMed

Berney, Cédric; Geisen, Stefan; Van Wichelen, Jeroen; Nitsche, Frank; Vanormelingen, Pieter; Bonkowski, Michael; Bass, David

2015-05-01

Amoebae able to form cytoplasmic networks or displaying a multiply branching morphology remain very poorly studied. We sequenced the small-subunit ribosomal RNA gene of 15 new amoeboid isolates, 14 of which are branching or network-forming amoebae (BNFA). Phylogenetic analyses showed that these isolates all group within the poorly-known and weakly-defined class Variosea (Amoebozoa). They are resolved into six lineages corresponding to distinct new morphotypes; we describe them as new genera Angulamoeba (type species Angulamoeba microcystivorans n. gen., n. sp.; and A. fungorum n. sp.), Arboramoeba (type species Arboramoeba reticulata n. gen., n. sp.), Darbyshirella (type species Darbyshirella terrestris n. gen., n. sp.), Dictyamoeba (type species Dictyamoeba vorax n. gen., n. sp.), Heliamoeba (type species Heliamoeba mirabilis n. gen., n. sp.), and Ischnamoeba (type species Ischnamoeba montana n. gen., n. sp.). We also isolated and sequenced four additional variosean strains, one belonging to Flamella, one related to Telaepolella tubasferens, and two members of the cavosteliid protosteloid lineage. We identified a further 104 putative variosean environmental clone sequences in GenBank, comprising up to 14 lineages that may prove to represent additional novel morphotypes. We show that BNFA are phylogenetically widespread in Variosea and morphologically very variable, both within and between lineages. Copyright © 2015 Elsevier GmbH. All rights reserved.

MitoGen: A Framework for Generating 3D Synthetic Time-Lapse Sequences of Cell Populations in Fluorescence Microscopy.

PubMed

Svoboda, David; Ulman, Vladimir

2017-01-01

The proper analysis of biological microscopy images is an important and complex task. Therefore, it requires verification of all steps involved in the process, including image segmentation and tracking algorithms. It is generally better to verify algorithms with computer-generated ground truth datasets, which, compared to manually annotated data, nowadays have reached high quality and can be produced in large quantities even for 3D time-lapse image sequences. Here, we propose a novel framework, called MitoGen, which is capable of generating ground truth datasets with fully 3D time-lapse sequences of synthetic fluorescence-stained cell populations. MitoGen shows biologically justified cell motility, shape and texture changes as well as cell divisions. Standard fluorescence microscopy phenomena such as photobleaching, blur with real point spread function (PSF), and several types of noise, are simulated to obtain realistic images. The MitoGen framework is scalable in both space and time. MitoGen generates visually plausible data that shows good agreement with real data in terms of image descriptors and mean square displacement (MSD) trajectory analysis. Additionally, it is also shown in this paper that four publicly available segmentation and tracking algorithms exhibit similar performance on both real and MitoGen-generated data. The implementation of MitoGen is freely available.

Terrestrial and lacustrine gastropods from the Priabonian (upper Eocene) of the Sultanate of Oman.

PubMed

Harzhauser, Mathias; Neubauer, Thomas A; Kadolsky, Dietrich; Pickford, Martin; Nordsieck, Hartmut

2016-01-01

Terrestrial and aquatic gastropods from the upper Eocene (Priabonian) Zalumah Formation in the Salalah region of the Sultanate of Oman are described. The assemblages reflect the composition of the continental mollusc fauna of the Palaeogene of Arabia, which, at that time, formed parts of the southeastern Tethys coast. Several similarities with European faunas are observed at the family level, but are rarer at the genus level. These similarities point to an Eocene (Priabonian) rather than to a Rupelian age, although the latter correlation cannot be entirely excluded. At the species level, the Omani assemblages lack any relations to coeval faunas. This suggests the possible presence of a distinct biogeographic province during the Palaeogene or may simply reflect the extremely sparse non-marine fossil record of the Eocene in the Tethys region. The occurrence of the genera Lanistes, Pila, and Gulella along with some pomatiids, probably related to extant genera, suggests that the modern African-Arabian continental faunas can be partly traced back to Eocene times and reflect very old autochthonous developments. In contrast, the diverse Vidaliellidae went extinct, and the morphologically comparable Neogene Achatinidae may have occupied the equivalent niches in extant environments. Carnevalea Harzhauser and Neubauer nov. gen., Arabiella Kadolsky, Harzhauser and Neubauer nov. gen., Pyrgulella Harzhauser, Kadolsky and Neubauer nov. gen., Salalahia Kadolsky, Harzhauser and Neubauer nov. gen., Omanitopsis Harzhauser and Neubauer nov. gen., Arabicolaria Harzhauser and Neubauer nov. gen., Pacaudiella Harzhauser and Neubauer nov. gen., Goniodomulus Harzhauser and Neubauer nov. gen., Eoquickia Harzhauser and Neubauer nov. gen., Omanillya H. Nordsieck nov. gen. and Omanifera H. Nordsieck nov. gen. are introduced as new genera. Pila neuberti Harzhauser and Neubauer nov. sp., Arabiella arabica Kadolsky, Harzhauser and Neubauer nov. sp., Pyrgulella parva Harzhauser, Kadolsky and Neubauer nov. sp., Salalahia thaytinitiensis Kadolsky, Harzhauser and Neubauer nov. sp., Omanitopsis vandammei Harzhauser and Neubauer nov. sp., Procyclotopsis eocenica Harzhauser and Neubauer nov. sp., Palaeocyclotus kuehschelmi Harzhauser and Neubauer nov. sp., Arabicolaria arabica Harzhauser and Neubauer nov. sp., Pacaudiella omanica Harzhauser and Neubauer nov. sp., Pacaudiella flammulata Harzhauser and Neubauer nov. sp., Goniodomulus solaniformis Harzhauser and Neubauer nov. sp., Cerastus hyznyi Harzhauser and Neubauer nov. sp., Omanillya lunellifera H. Nordsieck nov. sp., Omanillya costellata H. Nordsieck nov. sp., and Omanifera euclista H. Nordsieck nov. sp. are described as new species.

Diffusivity in Alumina Scales Grown on Al-MAX Phases

NASA Technical Reports Server (NTRS)

Smialek, James L.

2014-01-01

Ti3AlC2, Ti2AlC, and Cr2AlC are oxidation resistant MAX phase compounds distinguished by the formation of protective Al2O3 scales with well controlled kinetics. A modified Wagner treatment was used to obtain interfacial grain boundary diffusivity, deltaD(sub gb,O,int.), from scale growth rates and corresponding grain size. It is based on the p(O2)(exp -1/6) dependency of the double charged oxygen vacancy and oxygen diffusivity, coupled with the effective diffusion constant for short circuit grain boundary paths. Data from the literature for MAX phases was analyzed accordingly, and deltaD(sub gb,O,int.) was found to nearly coincide with the Arrhenius line developed for Zr-doped FeCrAl, where: deltaD(sub gb,O,int.) = 1.8x10(exp -10) exp(-375 kJ/RT) cubic meters/s. Furthermore, this oxidation relation suggests the more general format applicable to bulk samples under ambient conditions: deltaD(sub gb,O) = 7.567x10(exp -8) exp(-544 kJ/RT) p(O2)(exp -1/6) cubic meters/[s x Pa(exp -1/6)]. Data from many other FeCrAl(X) studies were similarly assessed to show general agreement with the relation for deltaD(sub gb,O,int.). This analysis reinforces the view that protective alumina scales grow by similar mechanisms for these Al-MAX phases and oxidation resistant FeCrAl alloys.

Population of Nitrifying Bacteria and Nitrification in Ammonium Saturated Clinoptilolite

NASA Technical Reports Server (NTRS)

McGilloway, R. L.; Weaver, R. W.; Ming, Douglas W.; Gruener, J.

1999-01-01

As humans begin to spend longer periods of time in space, plants will be incorporated into life support systems. Ammonium saturated clinoptilolite is one plant growth substrate but a balance between ammonium and nitrate is needed. A laboratory study was conducted to determine effects of nitrifying bacteria on ammonium concentrations and kinetics of nitrification. Columns containing clinoptilolite substrate amended with nitrifying bacteria obtained from soil enrichment were analyzed weekly for a 90 day period. The enrichment culture initially contained 1 x 10(exp 5) ammonium oxidizing bacteria and 1 x 10(exp 2) nitrite oxidizing bacteria per gram of substrate. Populations of ammonium oxidizing bacteria increased to 1 x 10(exp 6) and nitrite oxidizing bacteria increased to 1 x 10(exp 3) per gram of substrate. The nitrification rate was approximately 0.25mg NO3(-)-N/kg.hr. Experiments were also conducted to enumerate nitrifying bacteria in a clinoptilolite substrate used to grow wheat (Triticum aestivum L.). Seventy days following the initial inoculation with an unknown number of commercial nitrifying bacteria, 1 x 10(exp 5) ammonium oxidizing bacteria per gram of substrate were present. The number of nitrite oxidizing bacteria was between 1 x 10(exp 3) to 10(exp 4) per gram of substrate as measured by the most probable number method. Nitrification rates were approximately 0.20mg NO3(-)-N/kg.hr. Clinoptilolite readily exchanged sufficient concentrations of ammonium to support nitrifying bacteria and they survived well in this medium.

Improving Models for Coseismic And Postseismic Deformation from the 2002 Denali, Alaska Earthquake

NASA Astrophysics Data System (ADS)

Harper, H.; Freymueller, J. T.

2016-12-01

Given the multi-decadal temporal scale of postseismic deformation, predictions of previous models for postseismic deformation resulting from the 2002 Denali Fault earthquake (M 7.9) do not agree with longer-term observations. In revising the past postseismic models with what is now over a decade of data, the first step is revisiting coseismic displacements and slip distribution of the earthquake. Advances in processing allow us to better constrain coseismic displacement estimates, which affect slip distribution predictions in modeling. Additionally, an updated slip model structure from a homogeneous model to a layered model rectifies previous inconsistencies between coseismic and postseismic models. Previous studies have shown that two primary processes contribute to postseismic deformation: afterslip, which decays with a short time constant; and viscoelastic relaxation, which decays with a longer time constant. We fit continuous postseismic GPS time series with three different relaxation models: 1) logarithmic decay + exponential decay, 2) log + exp + exp, and 3) log + log + exp. A grid search is used to minimize total model WRSS, and we find optimal relaxation times of: 1) 0.125 years (log) and 21.67 years (exp); 2) 0.14 years (log), 0.68 years (exp), and 28.33 years (exp); 3) 0.055 years (log), 14.44 years (log), and 22.22 years (exp). While there is not a one-to-one correspondence between a particular decay constant and a mechanism, the optimization of these constants allows us to model the future timeseries and constrain the contribution of different postseismic processes.

Experimental and calculated LET distributions in the Cosmos-2044 biosatellite orbit

NASA Technical Reports Server (NTRS)

Watts, J. W., Jr.; Dudkin, V. E.; Karpov, O. N.; Potapov, Yu. V.; Akopova, A. B.; Magradze, N. V.; Moiseenko, A. A.; Benton, E. V.; Frank, A. L.

1995-01-01

During the flight of the Cosmos-2044 biosatellite, joint U.S.S.R.-U.S.A. investigations of different characteristics of cosmic radiation (CR) in the near-Earth environment were carried out. The U.S. dielectric track detectors CR-39 and Soviet BYa- and BR-type nuclear photo-emulsions were used as detectors. The present work shows some results of experimental measurements of linear energy transfer (LET) spectra of CR particles obtained with the use of these detectors, which were placed both inside and outside the satellite. The LET spectra measurements with plastic detectors is composed of two parts: the measurement of galactic cosmos rays (GCR) particles, and of short-range particles. The contributions of these components to the total LET distribution at various thicknesses of the shielding were analyzed and the results of these studies are presented. Calculated LET spectra in the Cosmos-2044 orbit were compared with experimental data. On the basis of experimental and calculated values of the LET spectra, absorbed and equivalent CR doses were calculated. In the shielding range of 1-1.5 g cm(exp -2), outside the spacecraft, the photo-emulsions yielded 10.3 mrad d(exp -1) and 13.4 mrem d(exp -1) (LET greater than or equal to 40 MeV cm(exp -1)). Inside the spacecraft (greater than or equal to 10 g cm(exp -2) the photo-emulsions yielded 8.9 mrad d(exp -1) and 14.5 mrem d(exp -1).

Traditional and confocal descriptions of a new genus and two new species of deep water Cerviniinae Sars, 1903 from the Southern Atlantic and the Norwegian Sea: with a discussion on the use of digital media in taxonomy (Copepoda, Harpacticoida, Aegisthidae).

PubMed

Corgosinho, Paulo H C; Kihara, Terue C; Schizas, Nikolaos V; Ostmann, Alexandra; Arbizu, Pedro Martínez; Ivanenko, Viatcheslav N

2018-01-01

Aegisthidae is one of the most abundant and diverse families of harpacticoid copepods living in deep-sea benthos, and the phylogenetic relationships within the family are in state of flux. Females of two new deep-water species of harpacticoid copepods belonging to the Hase gen. n. (Aegisthidae: Cerviniinae) are described. The first taxonomic description of marine copepod species based on the combined use of interference and confocal microscopy for the study of the habitus and dissected appendages is presented here. CLSM (Confocal Laser Scanning Microscopy) is a non-destructive method, comparable in quality to SEM (scanning electron microscopy) at the same magnifications. To observe and reconstruct in detail the habitus and dissected appendages, whole specimens and dissected parts were stained with Congo Red, mounted on slides with glycerine for CLSM and scanned under three visible-light lasers. Hase lagomorphicus gen. et sp. n. and Hase talpamorphicus gen. et sp. n. were collected from the sediments of the Southern Atlantic and the Norwegian Sea, from 2270 m and 5468 m depths, respectively. Hase gen. n. is included within Cerviniinae based on the caudal rami which are relatively divergent. Hase gen. n. is the sister taxon of Cerviniella based on the following synapomorphies: sturdy body, exopodites 1-3 of pereopods 1-3 heavily built, transformed into digging limbs, with strong outer and distal spines/setae, two-segmented endopod on the pereopods 2 and 3, and a reduced pereopod 5. Compared to Cerviniella, Hase gen. n. exhibits a more developed armature on the pereopod 1, which has outer and distal elements transformed into strong and long spines vs. stiff setae on Cerviniella.Hase gen. n. has one or two strong and long spines on the inner margin of the exopodite 3 of pereopod 4 and pereopod 5 is fused to the somite, ornamented with three distal setae. The telson of Hase gen. n. is subquadratic, and the furca is among the shortest yet described for Aegisthidae. The new species differ in a number of diagnostic characters, three of which are: a) the somite bearing pereopods 3 and 4 with latero-distal spiniform processes in H. talpamorphicus gen. et sp. n. but smooth in H. lagomorphicus gen. et sp. n. , b) antenna is armed with three stout spines on the lateral inner margin of the exopod in H. talpamorphicus gen. et sp. n. and two proximal setae in H. lagomorphicus gen. et sp. n. , and c) pereopod 4 exopodite 3 has two long and strong spines on the inner margin in H. lagomorphicus gen. et sp. n. and one spine in H. talpamorphicus gen. et sp. n. The high quality of CLSM images should foster discussion about the use of high quality digital images as type or as part of the type series in zoological studies, especially when studying rare and small macrofaunal and meiofaunal taxa.

Traditional and confocal descriptions of a new genus and two new species of deep water Cerviniinae Sars, 1903 from the Southern Atlantic and the Norwegian Sea: with a discussion on the use of digital media in taxonomy (Copepoda, Harpacticoida, Aegisthidae)

PubMed Central

Corgosinho, Paulo H. C.; Kihara, Terue C.; Schizas, Nikolaos V.; Ostmann, Alexandra; Arbizu, Pedro Martínez; Ivanenko, Viatcheslav N.

2018-01-01

Abstract Aegisthidae is one of the most abundant and diverse families of harpacticoid copepods living in deep-sea benthos, and the phylogenetic relationships within the family are in state of flux. Females of two new deep-water species of harpacticoid copepods belonging to the Hase gen. n. (Aegisthidae: Cerviniinae) are described. The first taxonomic description of marine copepod species based on the combined use of interference and confocal microscopy for the study of the habitus and dissected appendages is presented here. CLSM (Confocal Laser Scanning Microscopy) is a non-destructive method, comparable in quality to SEM (scanning electron microscopy) at the same magnifications. To observe and reconstruct in detail the habitus and dissected appendages, whole specimens and dissected parts were stained with Congo Red, mounted on slides with glycerine for CLSM and scanned under three visible-light lasers. Hase lagomorphicus gen. et sp. n. and Hase talpamorphicus gen. et sp. n. were collected from the sediments of the Southern Atlantic and the Norwegian Sea, from 2270 m and 5468 m depths, respectively. Hase gen. n. is included within Cerviniinae based on the caudal rami which are relatively divergent. Hase gen. n. is the sister taxon of Cerviniella based on the following synapomorphies: sturdy body, exopodites 1–3 of pereopods 1–3 heavily built, transformed into digging limbs, with strong outer and distal spines/setae, two-segmented endopod on the pereopods 2 and 3, and a reduced pereopod 5. Compared to Cerviniella, Hase gen. n. exhibits a more developed armature on the pereopod 1, which has outer and distal elements transformed into strong and long spines vs. stiff setae on Cerviniella.Hase gen. n. has one or two strong and long spines on the inner margin of the exopodite 3 of pereopod 4 and pereopod 5 is fused to the somite, ornamented with three distal setae. The telson of Hase gen. n. is subquadratic, and the furca is among the shortest yet described for Aegisthidae. The new species differ in a number of diagnostic characters, three of which are: a) the somite bearing pereopods 3 and 4 with latero-distal spiniform processes in H. talpamorphicus gen. et sp. n. but smooth in H. lagomorphicus gen. et sp. n., b) antenna is armed with three stout spines on the lateral inner margin of the exopod in H. talpamorphicus gen. et sp. n. and two proximal setae in H. lagomorphicus gen. et sp. n., and c) pereopod 4 exopodite 3 has two long and strong spines on the inner margin in H. lagomorphicus gen. et sp. n. and one spine in H. talpamorphicus gen. et sp. n. The high quality of CLSM images should foster discussion about the use of high quality digital images as type or as part of the type series in zoological studies, especially when studying rare and small macrofaunal and meiofaunal taxa. PMID:29930476

Unleashing Gen Y: Marketing Mars to Millennials

NASA Technical Reports Server (NTRS)

Leahy, Bart D.; Hidalgo, Loretta; Kloberdanz, Cassie

2007-01-01

Space advocates need to engage Generation Y (born 1977-1999).This outreach is necessary to recruit the next generation of scientists and engineers to explore Mars. Space advocates in the non-profit, private, and government sectors need to use a combination of technical communication, marketing, and politics, to develop messages that resonate with Gen Y. Until now, space messages have been generated by and for college-educated white males; Gen Y is much more diverse, including as much as one third minorities. Young women, too, need to be reached. My research has shown that messages emphasizing technology, fun, humor, and opportunity are the best means of reaching the Gen Y audience of 60 million (US population is 300 million). The important things space advocates must avoid are talking down to this generation, making false promises, or expecting them to "wait their turn" before they can participate. This is the MTV generation! We need to find ways of engaging Gen Y now to build a future where human beings can live and work on the planet Mars. In addition to the messages themselves, advocates need to keep up with Gen Y' s social networking and use of iPods, cell phones, and the Internet. NASA and space advocacy groups can use these tools for "viral marketing," where young people share targeted space-related information via cell phones or the Internet because they like it. Overall, Gen Y is a socially dynamic and media-savvy group; advocates' space messages need to be sincere, creative, and placed in locations where Gen Y lives. Mars messages must be memorable!

A survey of linyphiid spiders from Xishuangbanna, Yunnan Province, China (Araneae, Linyphiidae)

PubMed Central

Zhao, Qingyuan; Li, Shuqiang

2014-01-01

Abstract Eight new genera and 30 new species are described: Cirrosus gen. n. (type species Cirrosus atrocaudatus sp. n. (♂♀)), Conglin gen. n. (type species Conglin personatus sp. n. (♀)), Curtimeticus gen. n. (type species Curtimeticus nebulosus sp. n. (♂)), Gladiata gen. n. (type species Gladiata fengli sp. n. (♂)), Glebala gen. n. (type species Glebala aspera sp. n. (♂)), Glomerosus gen. n. (type species Glomerosus lateralis sp. n. (♂)), Smerasia gen. n. (type species Smerasia obscurus sp. n. (♂♀)), Vittatus gen. n. (type species Vittatus fencha sp. n. (♂♀)); Batueta cuspidata sp. n. (♂♀), Capsulia laciniosa sp. n. (♂), Dactylopisthes separatus sp. n. (♀), Gongylidiellum bracteatum sp. n. (♀), Houshenzinus xiaolongha sp. n. (♂♀), Laogone bai sp. n. (♂), Laogone lunata sp. n. (♂♀), Maro bulbosus sp. n. (♀), Nasoonaria circinata sp. n. (♂♀), Neriene circifolia sp. n. (♂♀), Oedothorax biantu sp. n. (♀), Oilinyphia hengji sp. n. (♂♀), Paikiniana furcata sp. n. (♂♀), Parameioneta bishou sp. n. (♂♀), Parameioneta multifida sp. n. (♂♀), Parameioneta tricolorata sp. n. (♂♀), Tapinopa undata sp. n. (♂), Theoa bidentata sp. n. (♂♀), Theoa vesica sp. n. (♂♀), Vittatus bian sp. n. (♂♀), Vittatus latus sp. n. (♂♀), Vittatus pan sp. n. (♂♀). The male of Kaestneria bicultrata Chen & Yin, 2000 and the females of Asiagone perforata Tanasevitch, 2014 and Batueta similis Wunderlich & Song, 1995 are described for the first time; photos of Bathyphantes paracymbialis Tanasevitch, 2014 are provided. PMID:25561858

Electrically conducting polyimide film containing tin complexes

NASA Technical Reports Server (NTRS)

St.clair, Anne K. (Inventor); Ezzell, Stephen A. (Inventor)

1994-01-01

Disclosed is a thermally-stable SnO2-surfaced polyimide film wherein the electrical conductivity of the SnO2 surface is within the range of about 3.0 x 10(exp -3) to about 1 x 10(exp -2) ohms(exp -1). Also disclosed is a method of preparing this film from a solution containing a polyamic acid and SnCl4 (DMSO)2.

On the number of multiplications necessary to compute a length-2 exp n DFT

NASA Technical Reports Server (NTRS)

Heideman, M. T.; Burrus, C. S.

1986-01-01

The number of multiplications necessary and sufficient to compute a length-2 exp n DFT is determined. The method of derivation is shown to apply to the multiplicative complexity results of Winograd (1980, 1981) for a length-p exp n DFT, for p an odd prime number. The multiplicative complexity of the one-dimensional DFT is summarized for many possible lengths.

In vitro comparison of implant- versus gingiva-supported removable dentures in anterior and posterior applications.

PubMed

Rosentritt, Martin; Heidtkamp, Felix; Hösl, Helmut; Hahnel, Sebastian; Preis, Verena

2016-03-01

Removable dentures with different denture teeth may provide different performance and resistance in implant and gingival situations, or anterior and posterior applications. Two situations of removable dentures were investigated: gingiva (flexible) and implant (rigid) bearing. For simulating the gingiva/jaw situation, the dentures were supported with flexible lining material. For the implant situation, implants (d = 4.1 mm) were screwed into polymethylenmethacrylate (PMMA) resin. Two commercial (Vita-Physiodens MRP, SR Vivodent/Orthotyp DCL) and two experimental materials (EXP1, EXP2) were investigated in anterior (A) and posterior (P) tooth locations. Chewing simulation was performed, and failures were analyzed (microscopy, SEM). Fracture strength of surviving dentures was determined. Only EXP1 revealed failures during chewing simulation. Failures varied between anterior and posterior locations, and between implant (P:4x; A:7x) or gingiva (P:1x; A:2x) situations. Kaplan-Meier log-rank test revealed significant differences for implant situations (p < 0.002), but not for gingiva bearing (p > 0.093). Fracture testing in the implant situation provided significantly highest values for EXP2 (1476.4 ± 532.2 N) in posterior location, and for DCL (1575.4 ± 264.4 N) and EXP2 (1797.0 ± 604.2 N) in anterior location. For gingival bearing, significantly highest values were found for DCL/P (2148.3 ± 836.3 N), and significantly lowest results for EXP1/A (308.2 ± 115.6 N). For EXP1 + EXP2 + Vita/P and for EXP1/A no significant differences were found between implant- or gingiva-supported situations. Anterior and posterior teeth showed different material-dependent in vitro performance, further influenced by implant/gingiva bearing. While an implant in anterior application increased fracture strength of two materials, it decreased fracture values of 3/4 of the materials in posterior application. Survival of denture teeth may be influenced by material, oral position, and bearing situation.

Titan's Far-Infrared 220 cm(exp -1) Cloud Seen for the First Time in the South

NASA Technical Reports Server (NTRS)

Jennings, Donald; Anderson, Carrie; Samuelson, Robert; Nixon, Conor; Flasar, Michael; Teanby, Nick; deKok, Remco; Coustenis, Athena; Vinatier, Sandrine

2013-01-01

In 2012 an emission feature at 220 cm(exp -1) in Titan's far-infrared spectrum was seen for the first time in the south. Attributed to a stratosphere ice cloud formed at the winter pole, the 220 (exp -1) emission had previously been seen only at high northern latitudes where it bad been decreasing since the arrival of Cassini in 2004. Our far-infrared observations were performed With the Composite Infrared Spectrometer (CIRS) on Caasini. Although it bad been expected that the 220 cm(exp -1) emission would eventnal1y appear in the south, the emission appeared rather suddenly, increasing by a factor of at least four between February (when it was not detected) and July 2012. At the time of our observations, one Titan month after equinox, the 220 cm(exp -1) feature was present in both the north and south and showed a trend of continued slow decrease in the north and steep increase in the south. As has been the case in the north, the emission in the south was confined to high latitudes associated with winter polar shadowing. Our spectroscopic detection of the southern 220 cm(exp -1) ice cloud coincided with the rapid formation in 2012 of a haze hood and vortex at the south pole as seen in Cassini image. The 220 cm(exp -1) feature was first observed by the Infrared Interferometer Spectrometer (IRIS) on Voyager I and has been extensively studied in the north by CIRS. Until now the 220 cm(exp -1) emission, like the polar hood, has been associated solely with the north, owing to the fact that Voyager and Cassini have viewed Titan only during winter-spring. In 2012 we witnessed the start of a seasonal shift of this pattern to the south. The 220 cm(exp -1) emission arises from altitudes of 80-150 km and peaks sharply near 140 km. The material responsible for the spectral feature is not known, but indirect evidence hints at a condensate arising from complex nitriles, which also tend to be present only at high winter latitudes.

Rubitecan: 9-NC, 9-Nitro-20(S)-camptothecin, 9-nitro-camptothecin, 9-nitrocamptothecin, RFS 2000, RFS2000.

PubMed

2004-01-01

Rubitecan [Orathecin, 9-nitrocamptothecin, 9NC, RFS 2000] is a topoisomerase I inhibitor extracted from the bark and leaves of the Camptotheca acuminata tree, which is native to China. Rubitecan is an oral compound being developed for the treatment of pancreatic cancer and other solid tumours by SuperGen. One of the major benefits of rubitecan is that it can be administered in an outpatient setting, so patients can be treated in their homes. Rubitecan was isolated by the Stehlin Foundation in the US. SuperGen is currently awaiting regulatory approval in the US and the EU for rubitecan in the treatment of pancreatic cancer. At the BIO-2004 conference, SuperGen announced it is seeking a partner for rubitecan for territories outside the US. SuperGen acquired exclusive worldwide rights to rubitecan from the Stehlin Foundation in 1997 except in Mexico, Canada, Spain, Japan, the UK, France, Italy and Germany. SuperGen has also received approval from the US FDA to use its own manufactured rubitecan in clinical trials. SuperGen and the Stehlin Foundation have an 8-year research agreement that secures global rights to other camptothecins and additional anticancer compounds for the former. In December 1999, SuperGen and Abbott signed a worldwide sales and marketing agreement for rubitecan. Under the terms of the agreement, Abbott had exclusive distribution and promotion rights for rubitecan outside the US, and co-promotion rights with SuperGen within the US. In return, Abbott made an initial equity investment in SuperGen. SuperGen and Abbott Laboratories ended their collaboration agreement in February 2002 by mutual consent with SuperGen stating that the dissolution of the agreement was based on commercial motivation rather than anything to do with rubitecan's safety or efficacy. Abbott no longer has rights or obligations to purchase shares of SuperGen stock or an option to purchase up to 49% of the company. For its part, SuperGen will no longer receive milestone payments worth up to $US57 million. SuperGen has formed a clinical and business alliance with US Oncology (created by the merger between American Oncology Resources and Physician Reliance Network in the US), and will collaborate on clinical trials of rubitecan. SuperGen believes that this relationship will increase the patient population available for trials and enable it to market the drug directly to Oncologists. SuperGen and Capital Research and Management Company have completed a $US16.6 million private placement transaction that will enable future funding for the rubitecan programme as well as other oncology programmes. In July 2004, SuperGen's European subsidiary, EuroGen Pharmaceuticals, submitted a Marketing Authorisation Application for rubitecan in the treatment of pancreatic cancer. The application will be reviewed under the EMEA Centralised Procedure. In June 2003, the EMEA granted SuperGen orphan drug status for rubitecan for the treatment of pancreatic cancer. The US FDA has also granted orphan drug status for rubitecan in the treatment of pancreatic cancer and fast-track status for rubitecan for the treatment of locally advanced or metastatic pancreatic cancer that is resistant or refractory to chemotherapy. SuperGen has conducted three phase III pivotal trials in patients with pancreatic cancer. A phase III randomised trial in chemotherapy-naive patients was conducted at 132 centres throughout the US. The trial enrolled approximately 994 patients who were randomised to receive rubitecan or gemcitabine. Enrollment was completed in October 2001. Another phase III trial has compared rubitecan with the most appropriate chemotherapy in chemotherapy-resistant patients. Enrollment of over 400 patients at 200 medical centres across the US was completed in June 2001. Results from the trial were presented at the 39th Annual Meeting of the American Society of Clinical Oncology (ASCO-2003) [Chicago, US; 31 May - 3 June 2003], after they had been compiled, analysed and submitted to the FDA. The results of the study showed that rubitecan could not help all chemotherapy-resistant patients, but could increase survival in those that do respond. The other phase III pivotal trial was conducted in patients with pancreatic cancer who had failed treatment with gemcitabine. This trial completed enrollment in October 2001, and had enrolled approximately 448 patients. SuperGen is conducting phase II trials of rubitecan in patients with solid tumours in the UK, Italy, France, Germany, the Netherlands and Denmark. Each trial will enroll 100-150 patients with various tumour types, including colorectal, lung, breast, gastric, prostate, cervical and head and neck cancers. Phase I/II trials are underway to investigate rubitecan as a radiosensitiser in patients with lung cancer, and phase II trials in patients with breast cancer are also being conducted. A phase II study in ovarian cancer patients is also being conducted. Results from an ongoing phase II study in cancer patients have shown that rubitecan was effective against chordomas, a rare type of bone cancer. Phase II studies are also underway in haematological malignancies including myelodysplastic syndrome (preleukaemia) and chronic myelomonocytic leukaemia. In February 2000, SuperGen announced that its IND submission for rubitecan had been approved by the Therapeutics Products Programme of Canada. The company stated that it intended to begin clinical trials in Canada in the near future. In February 2004, SuperGen announced an offering of shares of its common stock to finance the commercialisation of rubitecan capsules. In July 2003, SuperGen was granted a US patent covering combination therapies with chemotherapeutic anthracycline agents and structural modifications that may one day lead to next-generation rubitecan compounds. In December 2002, SuperGen was granted US patent No. 6,482,830, covering its polymorphic formulations of rubitecan. The patent also covers a class of polymorphs that are similar to the one at the centre of rubitecan. In addition, SuperGen was also issued US patent No. 6,485,514 in December 2002, covering the local delivery of rubitecan via stents and/or catheters to sites of proliferating cells. Stent- or catheter-delivered rubitecan may be beneficial in certain types of cardiac procedures, such as ablation or angioplasty, as well as for direct injection into a certain number of solid tumours. SuperGen is also developing an inhaled, liposomal formulation of rubitecan. It acquired the worldwide rights to this formulation from the Clayton Foundation in December 1999. Inhaled rubitecan is in clinical trials in the US for the treatment of lung cancer and pulmonary metastatic cancer. Copyright 2004 Adis Data Information BV

75 FR 59247 - Combined Notice of Filings #1

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... Segundo Power II LLC, Long Beach Generation LLC, Long Beach Peakers LLC, NRG Power Marketing Inc., Cabrillo Power I LLC, Cabrillo Power II LLC, El Segundo Power LLC, NRG Solar Blythe LLC, Saguaro Power... LLC, Dunkirk Power LLC, GenConn Energy LLC, GenCon Devon LLC, GenConn Middletown LLC, Huntley Power...

VitisGen on the road: mapping the way to the next generation of grapes

USDA-ARS?s Scientific Manuscript database

The USDA-NIFA VitisGen project (Award No. 2011-51181-30635) aims to optimize grape cultivar improvement through the integration of grape breeding, cutting edge genomics technology, precision phenotyping, socioeconomic research, and extension. VitisGen is a five-year project involving 25 co-PIs at 11...

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Science.gov Websites

from Air Force Gen. Lori Robinson during a ceremony held at Peterson Air Force Base today. May 24, 2018 Gen. O'Shaughnessy assumes command of NORAD, USNORTHCOM Peterson Air Force Base, Colo. - Air Force Gen terminal Sept. 22, 2017 at Joint Base San Antonio-Kelly Field. The cargo was being staged at the Federal

Complete cDNAs from Solenopsis invicta (Hymenoptera: Formicidae). --GenBank accession numbers: HM130684-HM130685.

USDA-ARS?s Scientific Manuscript database

2 new gene sequences were identified from workers of Solenopsis invicta, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are HM130684-HM130685. This information will provide scientists with genetic tools to study the populations of this ant....

Complete cDNAs from Brachymyrmex patagonicus (Hymenoptera: Formicidae). --GenBank accession numbers: GU582126-GU582140.

USDA-ARS?s Scientific Manuscript database

15 new gene sequences were identified from workers of Brachymyrmex patagonicus, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are GU582126-GU582140. This information will provide scientists with genetic tools to study the development and the p...

78 FR 77670 - AlphaGen Power LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-24

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. ER14-630-000] AlphaGen Power LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for Blanket Section 204 Authorization This is a supplemental notice in the above-referenced proceeding, of AlphaGen Power...

Safety Sufficiency for NextGen: Assessment of Selected Existing Safety Methods, Tools, Processes, and Regulations

NASA Technical Reports Server (NTRS)

Xu, Xidong; Ulrey, Mike L.; Brown, John A.; Mast, James; Lapis, Mary B.

2013-01-01

NextGen is a complex socio-technical system and, in many ways, it is expected to be more complex than the current system. It is vital to assess the safety impact of the NextGen elements (technologies, systems, and procedures) in a rigorous and systematic way and to ensure that they do not compromise safety. In this study, the NextGen elements in the form of Operational Improvements (OIs), Enablers, Research Activities, Development Activities, and Policy Issues were identified. The overall hazard situation in NextGen was outlined; a high-level hazard analysis was conducted with respect to multiple elements in a representative NextGen OI known as OI-0349 (Automation Support for Separation Management); and the hazards resulting from the highly dynamic complexity involved in an OI-0349 scenario were illustrated. A selected but representative set of the existing safety methods, tools, processes, and regulations was then reviewed and analyzed regarding whether they are sufficient to assess safety in the elements of that OI and ensure that safety will not be compromised and whether they might incur intolerably high costs.

GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research--an update.

PubMed

Peakall, Rod; Smouse, Peter E

2012-10-01

GenAlEx: Genetic Analysis in Excel is a cross-platform package for population genetic analyses that runs within Microsoft Excel. GenAlEx offers analysis of diploid codominant, haploid and binary genetic loci and DNA sequences. Both frequency-based (F-statistics, heterozygosity, HWE, population assignment, relatedness) and distance-based (AMOVA, PCoA, Mantel tests, multivariate spatial autocorrelation) analyses are provided. New features include calculation of new estimators of population structure: G'(ST), G''(ST), Jost's D(est) and F'(ST) through AMOVA, Shannon Information analysis, linkage disequilibrium analysis for biallelic data and novel heterogeneity tests for spatial autocorrelation analysis. Export to more than 30 other data formats is provided. Teaching tutorials and expanded step-by-step output options are included. The comprehensive guide has been fully revised. GenAlEx is written in VBA and provided as a Microsoft Excel Add-in (compatible with Excel 2003, 2007, 2010 on PC; Excel 2004, 2011 on Macintosh). GenAlEx, and supporting documentation and tutorials are freely available at: http://biology.anu.edu.au/GenAlEx. rod.peakall@anu.edu.au.

New Heteroceratidae (Ammonoidea) from the late Barremian deepening succession of Marseille (Bouches-du-Rhône, France)

NASA Astrophysics Data System (ADS)

Frau, Camille; Delanoy, Gérard; Masse, Jean-Pierre; Lanteaume, Cyprien; Tendil, Anthony J. B.

2016-06-01

Investigation of the late Barremian deepening succession of the Provence platform, cropping out south of Marseille (Bouches-du-Rhône, France), has yielded a new ammonite fauna belonging to the Martelites sarasini Subzone (Martelites sarasini Zone). The fauna is dominated by representatives of the Heteroceratidae Spath, characterized by different patterns of coiling, high intraspecific variabilities and dwarfism. These heteroceratids are distinctive and utterly different from all previously known taxa, and this justifies the introduction of the new taxa Heteroceras denizoti sp. nov., Heteroceras veratiae sp. nov., Calanquites gen. nov., based on Imerites katsharavai Rouchadzé; Giovaraites gen. nov., based on Giovaraites massiliae gen. et sp. nov., Barguesiella gen. nov., based on Barguesiella goudesense gen. et sp. nov. and the closely allied Barguesiella mantei gen. et sp. nov. The occurrence of the latter species at the top of the Maiolica Formation in Italy questions its early Aptian age assumed in the literature. The newly described fauna could be considered as the first case of micromorphy in the Heteroceratidae. Its biostratigraphy, palaeoenvironmental and palaeobiogeographical significance are discussed.

Decision validity should determine whether a generic or condition-specific HRQOL measure is used in health care decisions.

PubMed

Dowie, Jack

2002-01-01

When a HRQOL measure is needed in health care decision making should it be a generic measure (a GEN), intended to cover the entire domain of health, a condition-specific measure (a CSM) intended to embrace those aspects of health associated with the condition concerned, or both? This paper proposes that it will never be appropriate to use both a CSM and a GEN for the same decision; that a GEN alone will probably be the appropriate measure in the majority of decisions; that a CSM alone will sometimes be appropriate; and that whether it is a GEN alone or a CSM alone that is appropriate depends entirely on the structure of the decision. The argument rests on the distinction between knowledge validity and decision validity. But it has a supplementary basis in rejection of the widespread (but unjustifiable) belief that CSMs are more "sensitive" or "responsive" than GENs and hence can detect "small but important changes" that GENs always or often miss. Copyright 2002 John Wiley & Sons, Ltd.

Paramagnetic-to-nonmagnetic transition in antiperovskite nitride Cr3GeN studied by 14N-NMR and µSR

NASA Astrophysics Data System (ADS)

Takao, K.; Liu, Z.; Uji, K.; Waki, T.; Tabata, Y.; Watanabe, I.; Nakamura, H.

2017-06-01

The antiperovskite-related nitride Cr3GeN forms a tetragonal structure with the space group P\\bar{4}{2}1m at room temperature. It shows a tetragonal (P\\bar{4}{2}1m) to tetragonal (I4/mcm) structural transition with a large hysteresis at 300-400 K. The magnetic susceptibility of Cr3GeN shows Curie-Weiss type temperature dependence at high temperature, but is almost temperature-independent below room temperature. We carried out µSR and 14N-NMR microscopy measurements to reveal the magnetic ground state of Cr3GeN. Gradual muon spin relaxation, which is nearly temperature-independent below room temperature, was observed, indicating that Cr3GeN is magnetically inactive. In the 14N-NMR measurement, a quadrupole-split spectrum was obtained at around 14 K = 0. The temperature dependence of 14(1/T1) satisfies the Korringa relation. These experimental results indicate that the ground state of Cr3GeN is Pauli paramagnetic, without antiferromagnetic long-range order.

Fungal Planet description sheets: 469-557.

PubMed

Crous, P W; Wingfield, M J; Burgess, T I; Hardy, G E St J; Crane, C; Barrett, S; Cano-Lira, J F; Le Roux, J J; Thangavel, R; Guarro, J; Stchigel, A M; Martín, M P; Alfredo, D S; Barber, P A; Barreto, R W; Baseia, I G; Cano-Canals, J; Cheewangkoon, R; Ferreira, R J; Gené, J; Lechat, C; Moreno, G; Roets, F; Shivas, R G; Sousa, J O; Tan, Y P; Wiederhold, N P; Abell, S E; Accioly, T; Albizu, J L; Alves, J L; Antoniolli, Z I; Aplin, N; Araújo, J; Arzanlou, M; Bezerra, J D P; Bouchara, J-P; Carlavilla, J R; Castillo, A; Castroagudín, V L; Ceresini, P C; Claridge, G F; Coelho, G; Coimbra, V R M; Costa, L A; da Cunha, K C; da Silva, S S; Daniel, R; de Beer, Z W; Dueñas, M; Edwards, J; Enwistle, P; Fiuza, P O; Fournier, J; García, D; Gibertoni, T B; Giraud, S; Guevara-Suarez, M; Gusmão, L F P; Haituk, S; Heykoop, M; Hirooka, Y; Hofmann, T A; Houbraken, J; Hughes, D P; Kautmanová, I; Koppel, O; Koukol, O; Larsson, E; Latha, K P D; Lee, D H; Lisboa, D O; Lisboa, W S; López-Villalba, Á; Maciel, J L N; Manimohan, P; Manjón, J L; Marincowitz, S; Marney, T S; Meijer, M; Miller, A N; Olariaga, I; Paiva, L M; Piepenbring, M; Poveda-Molero, J C; Raj, K N A; Raja, H A; Rougeron, A; Salcedo, I; Samadi, R; Santos, T A B; Scarlett, K; Seifert, K A; Shuttleworth, L A; Silva, G A; Silva, M; Siqueira, J P Z; Souza-Motta, C M; Stephenson, S L; Sutton, D A; Tamakeaw, N; Telleria, M T; Valenzuela-Lopez, N; Viljoen, A; Visagie, C M; Vizzini, A; Wartchow, F; Wingfield, B D; Yurchenko, E; Zamora, J C; Groenewald, J Z

2016-12-01

Novel species of fungi described in this study include those from various countries as follows: Australia : Apiognomonia lasiopetali on Lasiopetalum sp., Blastacervulus eucalyptorum on Eucalyptus adesmophloia , Bullanockia australis (incl. Bullanockia gen. nov.) on Kingia australis , Caliciopsis eucalypti on Eucalyptus marginata , Celerioriella petrophiles on Petrophile teretifolia , Coleophoma xanthosiae on Xanthosia rotundifolia , Coniothyrium hakeae on Hakea sp., Diatrypella banksiae on Banksia formosa , Disculoides corymbiae on Corymbia calophylla , Elsinoë eelemani on Melaleuca alternifolia , Elsinoë eucalyptigena on Eucalyptus kingsmillii , Elsinoë preissianae on Eucalyptus preissiana , Eucasphaeria rustici on Eucalyptus creta , Hyweljonesia queenslandica (incl. Hyweljonesia gen. nov.) on the cocoon of an unidentified microlepidoptera, Mycodiella eucalypti (incl. Mycodiella gen. nov.) on Eucalyptus diversicolor , Myrtapenidiella sporadicae on Eucalyptus sporadica , Neocrinula xanthorrhoeae (incl. Neocrinula gen. nov.) on Xanthorrhoea sp., Ophiocordyceps nooreniae on dead ant, Phaeosphaeriopsis agavacearum on Agave sp., Phlogicylindrium mokarei on Eucalyptus sp., Phyllosticta acaciigena on Acacia suaveolens , Pleurophoma acaciae on Acacia glaucoptera , Pyrenochaeta hakeae on Hakea sp., Readeriella lehmannii on Eucalyptus lehmannii , Saccharata banksiae on Banksia grandis , Saccharata daviesiae on Daviesia pachyphylla , Saccharata eucalyptorum on Eucalyptus bigalerita , Saccharata hakeae on Hakea baxteri , Saccharata hakeicola on Hakea victoria , Saccharata lambertiae on Lambertia ericifolia , Saccharata petrophiles on Petrophile sp., Saccharata petrophilicola on Petrophile fastigiata , Sphaerellopsis hakeae on Hakea sp., and Teichospora kingiae on Kingia australis. Brazil : Adautomilanezia caesalpiniae (incl . Adautomilanezia gen. nov.) on Caesalpina echinata , Arthrophiala arthrospora (incl. Arthrophiala gen. nov.) on Sagittaria montevidensis , Diaporthe caatingaensis (endophyte from Tacinga inamoena ), Geastrum ishikawae on sandy soil, Geastrum pusillipilosum on soil, Gymnopus pygmaeus on dead leaves and sticks, Inonotus hymenonitens on decayed angiosperm trunk, Pyricularia urashimae on Urochloa brizantha , and Synnemellisia aurantia on Passiflora edulis . Chile : Tubulicrinis australis on Lophosoria quadripinnata. France : Cercophora squamulosa from submerged wood, and Scedosporium cereisporum from fluids of a wastewater treatment plant. Hawaii : Beltraniella acaciae , Dactylaria acaciae , Rhexodenticula acaciae , Rubikia evansii and Torula acaciae (all on Acacia koa ) . India : Lepidoderma echinosporum on dead semi-woody stems, and Rhodocybe rubrobrunnea from soil. Iran : Talaromyces kabodanensis from hypersaline soil. La Réunion : Neocordana musarum from leaves of Musa sp. Malaysia : Anungitea eucalyptigena on Eucalyptus grandis × pellita , Camptomeriphila leucaenae (incl. Camptomeriphila gen. nov.) on Leucaena leucocephala , Castanediella communis on Eucalyptus pellita , Eucalyptostroma eucalypti (incl. Eucalyptostroma gen. nov.) on Eucalyptus pellita , Melanconiella syzygii on Syzygium sp., Mycophilomyces periconiae (incl. Mycophilomyces gen. nov.) as hyperparasite on Periconia on leaves of Albizia falcataria , Synnemadiella eucalypti (incl. Synnemadiella gen. nov.) on Eucalyptus pellita , and Teichospora nephelii on Nephelium lappaceum. Mexico : Aspergillus bicephalus from soil. New Zealand : Aplosporella sophorae on Sophora microphylla , Libertasomyces platani on Platanus sp., Neothyronectria sophorae (incl. Neothyronectria gen. nov.) on Sophora microphylla , Parastagonospora phoenicicola on Phoenix canariensis , Phaeoacremonium pseudopanacis on Pseudopanax crassifolius , Phlyctema phoenicis on Phoenix canariensis , and Pseudoascochyta novae-zelandiae on Cordyline australis. Panama : Chalara panamensis from needle litter of Pinus cf. caribaea . South Africa : Exophiala eucalypti on leaves of Eucalyptus sp., Fantasmomyces hyalinus (incl. Fantasmomyces gen. nov.) on Acacia exuvialis , Paracladophialophora carceris (incl. Paracladophialophora gen. nov.) on Aloe sp., and Umthunziomyces hagahagensis (incl. Umthunziomyces gen. nov.) on Mimusops caffra. Spain : Clavaria griseobrunnea on bare ground in Pteridium aquilinum field, Cyathus ibericus on small fallen branches of Pinus halepensis , Gyroporus pseudolacteus in humus of Pinus pinaster , and Pseudoascochyta pratensis (incl. Pseudoascochyta gen. nov.) from soil. Thailand : Neoascochyta adenii on Adenium obesum , and Ochroconis capsici on Capsicum annuum . UK : Fusicolla melogrammae from dead stromata of Melogramma campylosporum on bark of Carpinus betulus . Uruguay : Myrmecridium pulvericola from house dust. USA : Neoscolecobasidium agapanthi (incl. Neoscolecobasidium gen. nov.) on Agapanthus sp . , Polyscytalum purgamentum on leaf litter, Pseudopithomyces diversisporus from human toenail, Saksenaea trapezispora from knee wound of a soldier, and Sirococcus quercus from Quercus sp . Morphological and culture characteristics along with DNA barcodes are provided.

Dedicated Caravan Sites for French Gens du Voyage: Public Health Policy or Construction of Health and Environmental Inequalities?

PubMed

Foisneau, Lise

2017-12-01

In France, gens du voyage ("people who travel" or "travellers") is a term used by the government to categorize various itinerant populations, the majority of which are diverse Romani groups. People categorized as gens du voyage are legally required to reside in particular locations called "dedicated caravan sites." Parliamentary debates about these dedicated caravan sites have clarified that one of the objectives of such sites is to help fulfill the gens du voyage 's right to health. However, there is a significant gap between the officially stated goals of such sites and the reality of life within them. This paper draws on research finding that the conditions in most dedicated caravan sites do not conform with the rights of gens du voyage to acceptable sanitary conditions and other underlying determinants of health.

Genistein and daidzein stimulate hyaluronic acid production in transformed human keratinocyte culture and hairless mouse skin.

PubMed

Miyazaki, Kouji; Hanamizu, Tomoko; Iizuka, Ryoko; Chiba, Katsuyoshi

2002-01-01

We examined the effects of the soy isoflavones genistein (Gen) and daidzein (Dai) on the production of hyaluronic acid (HA) in a transformed human keratinocyte culture and in hairless mouse skin following topical application for 2 weeks. Gen and Dai, but not the glycosides thereof, significantly enhanced the production of HA in vitro and in vivo. Histochemistry using an HA-binding protein revealed that topical Gen and estradiol raised both the density and intensity of HA staining, which was abundant in the murine dermis. It is suggested that Gen and Dai are not released from their respective glycosides in culture or murine skin. Moreover, topical Gen and Dai may prevent and improve the cutaneous alterations caused by the loss of HA in skin. Copyright 2002 S. Karger AG, Basel

Performance Analysis of Combined Methods of Genetic Algorithm and K-Means Clustering in Determining the Value of Centroid

NASA Astrophysics Data System (ADS)

Adya Zizwan, Putra; Zarlis, Muhammad; Budhiarti Nababan, Erna

2017-12-01

The determination of Centroid on K-Means Algorithm directly affects the quality of the clustering results. Determination of centroid by using random numbers has many weaknesses. The GenClust algorithm that combines the use of Genetic Algorithms and K-Means uses a genetic algorithm to determine the centroid of each cluster. The use of the GenClust algorithm uses 50% chromosomes obtained through deterministic calculations and 50% is obtained from the generation of random numbers. This study will modify the use of the GenClust algorithm in which the chromosomes used are 100% obtained through deterministic calculations. The results of this study resulted in performance comparisons expressed in Mean Square Error influenced by centroid determination on K-Means method by using GenClust method, modified GenClust method and also classic K-Means.

Dedicated Caravan Sites for French Gens du Voyage

PubMed Central

2017-01-01

Abstract In France, gens du voyage (“people who travel” or “travellers”) is a term used by the government to categorize various itinerant populations, the majority of which are diverse Romani groups. People categorized as gens du voyage are legally required to reside in particular locations called “dedicated caravan sites.” Parliamentary debates about these dedicated caravan sites have clarified that one of the objectives of such sites is to help fulfill the gens du voyage’s right to health. However, there is a significant gap between the officially stated goals of such sites and the reality of life within them. This paper draws on research finding that the conditions in most dedicated caravan sites do not conform with the rights of gens du voyage to acceptable sanitary conditions and other underlying determinants of health. PMID:29302165

The X-ray Reflectors in the Nucleus of the Seyfert Galaxy NGC 1068

NASA Technical Reports Server (NTRS)

Colbert, Edward J. M.; Weaver, Kimberly A.; Krolik, Julian H.; Mulchaey, John S.; Mushotzky, Richard F.; White, Nicholas E. (Technical Monitor)

2002-01-01

Based on observations of the Seyfert nucleus in NGC 1068 with ASCA, RXTE and BeppoSAX, we report the discovery of a flare (increase in flux by a factor of approximately 1.6) in the 6.7 keV Fe K line component between observations obtained four months apart, with no significant change in the other (6.21, 6.4, and 6.97 keV) Fe Kalpha line components. During this time, the continuum flux decreased by approximately 20%. The RXTE spectrum requires an Fe K absorption edge near 8.6 keV (Fe XXIII- XXV). The spectral data indicate that the 2-10 keV continuum emission is dominated (approximately 2/3 of the luminosity) by reflection from a previously unidentified region of warm, ionized gas located approximately or less than 0.2 pc from the AGN. The remaining approximately 1/3 of the observed X-ray emission is reflected from optically thick, neutral gas. The coronal gas in the inner Narrow-Line Region (NLR) and/or the cold gas at the inner surface of the obscuring 'torus' are possible cold reflectors. The inferred properties of the warm reflector are: size (diameter) approximately or less than 0.2 pc, gas density n approximately or greater than 10(exp 5.5)/cu cm, ionization parameter xi is approximately 10(exp 3.5) erg cm s(exp -1), and covering fraction 0.003 (L(sub 0)/ 10(exp 43.5) erg s(exp -1)(exp -1) less than (omega/4pi) less than 0.024 (L(sub 0)/ 10(exp 43.5) erg s(exp -1) (exp -1) where L(sub 0) is the intrinsic 2-10 keV X-ray luminosity of the AGN. We suggest that the warm reflector gas is the source of the (variable) 6.7 keV Fe line emission, and the 6.97 keV Fe line emission. The 6.7 keV line flare is assumed to be due to an increase in the emissivity of the warm reflector gas from a decrease (by 20-30%) in L(sub 0). The properties of the warm reflector are most consistent with an intrinsically X-ray weak AGN with L(sub 0) approximately equals 10(exp 43.0) erg s(exp -1). The optical and UV emission that scatters from the warm reflector into our line of sight is required to suffer strong extinction, which can be reconciled if the line-of-sight skims the outer surface of the torus. Thermal bremsstrahlung radio emission from the warm reflector may be detectable in VLBA radio maps of the NGC 1068 nucleus.

Reassessment and division of the genus Agraecia Audinet-Serville (Orthoptera: Tettigoniidae: Conocephalinae: Agraeciini).

PubMed

Chamorro-Rengifo, Juliana; Braun, Holger; Lopes-Andrade, Cristiano

2015-07-29

Agraecia Audinet-Serville, the type-genus of Agraeciini, comprises fifteen species names: thirteen used for extant species, one junior synonym, and one fossil. The species are morphologically very dissimilar, and were collected and described from different localities of the world. The genus was reassessed based on recently collected specimens from Brazil and Argentina as well as museum specimens. Based on morphological characteristics we re-delimit Agraecia sensu novo, keeping only two of the previously assigned species: A. punctata Saint-Fargeau & Audinet-Serville and A. dorsalis Karny. The subtribe Agraeciina subtrib. nov. is defined, which include Agraecia s. nov. and three new genera (Iaratrox Chamorro-Rengifo & Lopes-Andrade gen. nov., Starkonsa Chamorro-Rengifo & Lopes-Andrade gen. nov., and Yvelinula Chamorro-Rengifo & Lopes-Andrade gen. nov.). Three additional genera treated here, Parasubria Karny, Ragoniella Chamorro-Rengifo & Lopes-Andrade gen. nov., Redtenbachus Chamorro-Rengifo & Lopes-Andrade gen. nov. and Sylvainhugiella Chamorro-Rengifo & Lopes-Andrade gen. nov. currently remain unclassified inside Agraeciini. We desig-nate a neotype and describe the female and male for A. punctata. Bertoniella Rehn is proposed as a junior synonym of Agraecia, and Parasubria ziczac Karny as syn. nov. of Parasubria vittipes (Redtenbacher) comb. nov. The fossil species is transferred to Senexefigia† Chamorro-Rengifo & Lopes-Andrade gen. nov. We also describe two new species of Iaratrox gen. nov., I. brasilienses Chamorro-Rengifo & Lopes-Andrade sp. nov. and I. longicornia Chamorro-Rengifo & Lopes-Andrade sp. nov. The holotype of Agraecia fallax Karny nomen dubium seems to be lost and the syntypes of Agraecia festae Griffini are currently unavailable for examination, therefore, these two species are treated as incertae sedis, and should probably be transferred. A key to the studied genera is provided, as well as a key to species of Agraecia s. nov. and Iaratrox gen. nov. The calling songs of Parasubria vittipes (Redtenbacher) comb. nov. and Ragoniella pulchella (Hebard) comb. nov. are described. Numerous morphological details and some natural history aspects are discussed.

Super-Resolving Properties of Metallodielectric Stacks

DTIC Science & Technology

2010-01-01

i i B A   TMM formalism 11332211 ... += nnn TPTPTPTPTF        2221 1211 ff ff F 2 11 2 11 21 1, f T f f R ==)exp())exp()exp(( xiziBziAE...a b s (H )2 i n a .u Plot of the Magnetic field squared across the thickness of MDS1 consisting of Ag and GaP layers. There is an overlay of the...photonic band gaps)”         l  i ZR r i Zw r Zw w tEtrZE )()( exp )( )(),,( 2 2 2 0 0 l  20

Physics of systematic frequency variations in hydrogen masers

NASA Technical Reports Server (NTRS)

Mattison, Edward M.

1990-01-01

The frequency stability of hydrogen masers for intervals longer that 10(exp 4) seconds is limited at present by systematic processes. Researchers discuss the physics of frequency-determining mechanisms internal to the maser that are susceptible to systematic variations, and the connections between these internal mechanisms and external environmental factors. Based upon estimates of the magnitudes of systematic effects, they find that the primary internal mechanisms currently limiting long-term maser frequency stability are cavity pulling, at the level parts in 10(exp 15) per day, and wall shift variations, at the level of parts in 10(exp 16) to parts in 10(exp 15) per day. They discuss strategies for reducing systematic frequency variations.

ExpEdit: a webserver to explore human RNA editing in RNA-Seq experiments.

PubMed

Picardi, Ernesto; D'Antonio, Mattia; Carrabino, Danilo; Castrignanò, Tiziana; Pesole, Graziano

2011-05-01

ExpEdit is a web application for assessing RNA editing in human at known or user-specified sites supported by transcript data obtained by RNA-Seq experiments. Mapping data (in SAM/BAM format) or directly sequence reads [in FASTQ/short read archive (SRA) format] can be provided as input to carry out a comparative analysis against a large collection of known editing sites collected in DARNED database as well as other user-provided potentially edited positions. Results are shown as dynamic tables containing University of California, Santa Cruz (UCSC) links for a quick examination of the genomic context. ExpEdit is freely available on the web at http://www.caspur.it/ExpEdit/.

The Development of Predictive Engineering Formulations for Diver Heating. Volume 2.

DTIC Science & Technology

1982-01-01

0 DO 1132 Min14,25 C DROP VALUE & WEIGHT FROM SUM IF ’T’ VALUE <- 0.0 IF (EXP(M).LE.0. 0) GO TO 1132 TDMS-TDMS+(WGT(M-13)*EXP(M)) SWOT -SWGT+WGT (M- 13...1132 CONTINUE IF ( SWOT . EQ. 0. 0) GO TO 11321 TDMS-TDMS/SWGT 11321 P(I,1)-EXP(1) WRITE (LO, 1133) EXP(1-) 1133 FORMAT (’ EXPERIMENTAL TIME: ’,F8.2...I AND J ARE TEMPORARY VARIABLES USED 33 AS DO LOOP INDICIES AND COUNTERS. INTEGER COR1 BR COMMON /BODY/B(9,3),COR(9,2),WGT(12) TR0. 0 SWOT -O. 0 !NOTE

Explicit solution of integrated 1 - exp equation for predicting accumulation and decline of concentrations for drugs obeying nonlinear saturation kinetics.

PubMed

Keller, Frieder; Hartmann, Bertram; Czock, David

2009-12-01

To describe nonlinear, saturable pharmacokinetics, the Michaelis-Menten equation is frequently used. However, the Michaelis-Menten equation has no integrated solution for concentrations but only for the time factor. Application of the Lambert W function was proposed recently to obtain an integrated solution of the Michaelis-Menten equation. As an alternative to the Michaelis-Menten equation, a 1 - exp equation has been used to describe saturable kinetics, with the advantage that the integrated 1 - exp equation has an explicit solution for concentrations. We used the integrated 1 - exp equation to predict the accumulation kinetics and the nonlinear concentration decline for a proposed fictive drug. In agreement with the recently proposed method, we found that for the integrated 1 - exp equation no steady state is obtained if the maximum rate of change in concentrations (Vmax) within interval (Tau) is less than the difference between peak and trough concentrations (Vmax x Tau < C peak - C trough).

Long-term survival of bacterial spores in space

NASA Technical Reports Server (NTRS)

Horneck, G.; Bucker, H.; Reitz, G.

1994-01-01

On board of the NASA Long Duration Exposure Facility (LDEF), spores of Bacillus subtilis in monolayers (10(exp 6)/sample) or multilayers (10(exp 8)/sample) were exposed to the space environment for nearly six years and their survival was analyzed after retrieval. The response to space parameters, such as vacuum (10(exp -6) Pa), solar electromagnetic radiation up to the highly energetic vacuum-ultraviolet range 10(exp 9) J/sq m) and/or cosmic radiation (4.8 Gy), was studied and compared to the results of a simultaneously running ground control experiment. If shielded against solar ultraviolet (UV)-radiation, up to 80% of spores in multilayers survive in space. Solar UV-radiation, being the most deleterious parameter of space, reduces survival by 4 orders of magnitude or more. However, up to 10(exp 4) viable spores were still recovered, even in completely unprotected samples. Substances, such as glucose or buffer salts serve as chemical protectants. With this 6 year study in space, experimental data are provided to the discussion on the likelihood of 'Panspermia'.

Asymmetries in Infants’ Attention Toward and Categorization of Male Faces: The Potential Role of Experience

PubMed Central

Rennels, Jennifer L.; Kayl, Andrea J.; Langlois, Judith H.; Davis, Rachel E.; Orlewicz, Mateusz

2015-01-01

Infants typically have a preponderance of experience with females, resulting in visual preferences for female faces, particularly high attractive females, and in better categorization of female relative to male faces. We examined whether these abilities generalized to infants’ visual preferences for and categorization of perceptually similar male faces (i.e., low masculine males). Twelve-month-olds visually preferred high attractive relative to low attractive male faces within low masculine pairs only (Exp. 1), but did not visually prefer low masculine relative to high masculine male faces (Exp. 2). Lack of visual preferences was not due to infants’ inability to discriminate between the male faces (Exps. 3 & 4). Twelve-month-olds categorized low masculine, but not high masculine, male faces (Exp. 5). Infants could individuate male faces within each of the categories (Exp. 6). Twelve-month-olds’ attention toward and categorization of male faces may reflect a generalization of their female facial expertise. PMID:26547249

Baffin Bay Ice Drift and Export: 2002-2007

NASA Technical Reports Server (NTRS)

Kwok, Ron

2007-01-01

Multiyear estimates of sea ice drift in Baffin Bay and Davis Strait are derived for the first time from the 89 GHz channel of the AMSR-E instrument. Uncertainties in the drift estimates, assessed with Envisat ice motion, are approximately 2-3 km/day. A persistent atmospheric trough, between the coast of Greenland and Baffin Island, drives the prevailing southward drift pattern with average daily displacements in excess of 18-20 km during winter. Over the 5-year record, the ice export ranges between 360 and 675 x 10(exp 3) km(exp 2), with an average of 530 x 10(exp 3) km(exp 2). Sea ice area inflow from the Nares Strait, Lancaster Sound and Jones Sound potentially contribute up to a third of the net area outflow while ice production at the North Water Polynya contributes the balance. Rough estimates of annual volume export give approximately 500-800 km(exp 3). Comparatively, these are approximately 70% and approximately 30% of the annual area and Strait.

One-equation near-wall turbulence modeling with the aid of direct simulation data

NASA Technical Reports Server (NTRS)

Rodi, W.; Mansour, N. N.

1990-01-01

The length scales appearing in the relations for the eddy viscosity and dissipation rate in one-equation models were evaluated from direct numerical simulation data for developed channel and boundary-layer flow at two Reynolds numbers each. To prepare the ground for the evaluation, the distribution of the most relevant mean-flow and turbulence quantities is presented and discussed with respect to Reynolds-number influence and to differences between channel and boundary-layer flow. An alternative model is also examined in which bar-(v'(exp 2))(exp 1/2) is used as velocity scale instead of k(exp 1/2). With this velocity scale, the length scales now appearing in the model follow very closely a linear relationship near the wall so that no damping is necessary. For the determination of bar-v'(exp 2) in the context of a one-equation model, a correlation is provided between bar-(v'(exp 2))/k and bar-(u'v')/k.

77 FR 43301 - Medicaid Program; Disproportionate Share Hospital Allotments and Institutions for Mental Diseases...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-24

... increase in the CPI-U for the prior FY (0.0 percent). Column F FY 2010 TC MAP Exp. Incl. DSH. This column... including DSH expenditures. Column G FY 2010 TC MAP Exp. Net of DSH. This column contains the amount of the States' actual FY 2010 total computable DSH expenditures. Column H FY 2010 TC MAP Exp. Net of DSH. This...

The 105-Month Swift-BAT All-Sky Hard X-Ray Survey

NASA Technical Reports Server (NTRS)

Oh, Kyuseok; Koss, Michael; Markwardt, Craig B.; Schawinski, Kevin; Baumgartner, Wayne H.; Barthelmy, Scott D.; Cenko, S. Bradley; Gehrels, Neil; Mushotzky, Richard; Petulante, Abigail;

A very low resistance, non-sintered contact system for use on indium phosphide concentrator/shallow junction solar cells

NASA Technical Reports Server (NTRS)

Weizer, Victor G.; Fatemi, Navid S.

1991-01-01

An investigation is made into the possibility of providing low resistance contacts to shallow junction InP solar cells which do not require sintering and which do not cause device degradation even when subjected to extended annealing at elevated temperatures. We show that the addition of In to Au contacts in amounts that exceed the solid solubility limit lowers the as-fabricated (unsintered) contact resistivity (R sub c) to the 10(exp -5) ohm cm(exp 2) range. We next consider the contact system Au/Au2P3 which has been shown to exhibit as-fabricated R sub c values in the 10(exp -6) ohm cm(exp 2) range, but which fails quickly when heated. We show that the substitution of a refractory metal (W, Ta) for Au preserves the low R sub c values while preventing the destructive reactions that would normally take place in this system at high temperatures. We show, finally, that R sub c values in the 10(exp -7) ohm cm(exp 2) range can be achieved without sintering by combining the effects of In or Ga additions to Au contacts with the effects of introducing a thin Au2P3 layer at the metal-InP interface.

Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins

NASA Astrophysics Data System (ADS)

Joshi, Janak Raj; Khazanov, Netaly; Senderowitz, Hanoch; Burdman, Saul; Lipsky, Alexander; Yedidia, Iris

2016-12-01

Quorum sensing (QS) is a population density-dependent regulatory system in bacteria that couples gene expression to cell density through accumulation of diffusible signaling molecules. Pectobacteria are causal agents of soft rot disease in a range of economically important crops. They rely on QS to coordinate their main virulence factor, production of plant cell wall degrading enzymes (PCWDEs). Plants have evolved an array of antimicrobial compounds to anticipate and cope with pathogens, of which essential oils (EOs) are widely recognized. Here, volatile EOs, carvacrol and eugenol, were shown to specifically interfere with QS, the master regulator of virulence in pectobacteria, resulting in strong inhibition of QS genes, biofilm formation and PCWDEs, thereby leading to impaired infection. Accumulation of the signal molecule N-acylhomoserine lactone declined upon treatment with EOs, suggesting direct interaction of EOs with either homoserine lactone synthase (ExpI) or with the regulatory protein (ExpR). Homology models of both proteins were constructed and docking simulations were performed to test the above hypotheses. The resulting binding modes and docking scores of carvacrol and eugenol support potential binding to ExpI/ExpR, with stronger interactions than previously known inhibitors of both proteins. The results demonstrate the potential involvement of phytochemicals in the control of Pectobacterium.

Toward a Better Understanding of the Relationship between Belief in the Paranormal and Statistical Bias: The Potential Role of Schizotypy

PubMed Central

Dagnall, Neil; Denovan, Andrew; Drinkwater, Kenneth; Parker, Andrew; Clough, Peter

2016-01-01

The present paper examined relationships between schizotypy (measured by the Oxford-Liverpool Inventory of Feelings and Experience; O-LIFE scale brief), belief in the paranormal (assessed via the Revised Paranormal Belief Scale; RPBS) and proneness to statistical bias (i.e., perception of randomness and susceptibility to conjunction fallacy). Participants were 254 volunteers recruited via convenience sampling. Probabilistic reasoning problems appeared framed within both standard and paranormal contexts. Analysis revealed positive correlations between the Unusual Experience (UnExp) subscale of O-LIFE and paranormal belief measures [RPBS full scale, traditional paranormal beliefs (TPB) and new age philosophy]. Performance on standard problems correlated negatively with UnExp and belief in the paranormal (particularly the TPB dimension of the RPBS). Consideration of specific problem types revealed that perception of randomness associated more strongly with belief in the paranormal than conjunction; both problem types related similarly to UnExp. Structural equation modeling specified that belief in the paranormal mediated the indirect relationship between UnExp and statistical bias. For problems presented in a paranormal context a framing effect occurred. Whilst UnExp correlated positively with conjunction proneness (controlling for perception of randomness), there was no association between UnExp and perception of randomness (controlling for conjunction). PMID:27471481

Carbon dioxide clearance in rabbits during expiratory phase intratracheal pulmonary ventilation.

PubMed

Meyappan, Raju T; Raszynski, Andre; Bohorquez, Jorge; Totapally, Balagangadhar R; Koul, Pulin B; Norozian, Faraz M; Valcourt, Karl; Torbati, Dan

2007-01-01

The purpose of this study was to compare the efficacy of CO2 removal during conventional mechanical ventilation (CMV) with and without expiratory phase intratracheal pulmonary ventilation (expiratory ITPV or Exp-ITPV); and to compare CO2 clearance during Exp-ITPV, in pressure-controlled ventilation (PCV) and in volume-controlled ventilation (VCV) modes. Seven anesthetized rabbits were tracheotomized and intubated using a 4 mm endotracheal tube. Venous and arterial lines were established. The rabbits were paralyzed, mechanically ventilated, and ventilation parameters were adjusted to achieve baseline arterial hypercapnia. Animals were then ventilated during 30-minute trials of CMV and Exp-ITPV, in both PCV and VCV modes. A custom-built, microprocessor-controlled solenoid valve was used to limit ITPV gas flow to the expiratory phase. Proximal and carinal airway pressures and hemodynamic variables were continuously recorded, and arterial blood gases were analyzed at the end of each trial. Exp-ITPV, as compared with CMV, reduced arterial PCO2 by 12% and 21% in PCV and VCV modes, respectively (p < 0.02 and p < 0.001; one-sided paired t test), without significant changes in other cardiorespiratory variables. In conclusion, Exp-ITPV is more effective than CMV in clearing CO2 through a small endotracheal tube. Exp-ITPV is also more effective in VCV mode than PCV mode.

Cooling of solar flares plasmas. 1: Theoretical considerations

NASA Technical Reports Server (NTRS)

Cargill, Peter J.; Mariska, John T.; Antiochos, Spiro K.

1995-01-01

Theoretical models of the cooling of flare plasma are reexamined. By assuming that the cooling occurs in two separate phase where conduction and radiation, respectively, dominate, a simple analytic formula for the cooling time of a flare plasma is derived. Unlike earlier order-of-magnitude scalings, this result accounts for the effect of the evolution of the loop plasma parameters on the cooling time. When the conductive cooling leads to an 'evaporation' of chromospheric material, the cooling time scales L(exp 5/6)/p(exp 1/6), where the coronal phase (defined as the time maximum temperature). When the conductive cooling is static, the cooling time scales as L(exp 3/4)n(exp 1/4). In deriving these results, use was made of an important scaling law (T proportional to n(exp 2)) during the radiative cooling phase that was forst noted in one-dimensional hydrodynamic numerical simulations (Serio et al. 1991; Jakimiec et al. 1992). Our own simulations show that this result is restricted to approximately the radiative loss function of Rosner, Tucker, & Vaiana (1978). for different radiative loss functions, other scaling result, with T and n scaling almost linearly when the radiative loss falls off as T(exp -2). It is shown that these scaling laws are part of a class of analytic solutions developed by Antiocos (1980).

Measurement of the decay rate of the SiH feature as a function of temperature

NASA Technical Reports Server (NTRS)

Nuth, Joseph A., III; Kraus, George F.

1994-01-01

We have previously suggested that the SiH fundamental stretch could serve as a diagnostic indicator of the oxidation state of silicate surfaces exposed to the solar wind for prolonged periods. We have now measured the primary decay rate of SiH in vacuo as a function of temperature and find that the primary rate constant for the decay can be characterized by the following equation: k(min(exp -1)) approximately equals 0.186 exp(-9/RT) min(exp -1), where R = 2 x 10(exp -3) kcal deg(exp -1) mole(exp -1). This means that the half-life for the decay of the SiH feature at room temperature is approximately 20 yrs, whereas the half-life at a peak lunar regolith temperature of approximately 500K would be only approximately 20 days. At the somewhat lower temperature of approximately 400K the half-life for the decay is on the order of 200 days. The rate of loss of SiH as a function of temperature provides an upper limit to the quantity of H implanted by the solar wind which can be retained by a silicate grain in a planetary regolith. This will be discussed in more detail here.

Toward a Better Understanding of the Relationship between Belief in the Paranormal and Statistical Bias: The Potential Role of Schizotypy.

PubMed

Dagnall, Neil; Denovan, Andrew; Drinkwater, Kenneth; Parker, Andrew; Clough, Peter

2016-01-01

The present paper examined relationships between schizotypy (measured by the Oxford-Liverpool Inventory of Feelings and Experience; O-LIFE scale brief), belief in the paranormal (assessed via the Revised Paranormal Belief Scale; RPBS) and proneness to statistical bias (i.e., perception of randomness and susceptibility to conjunction fallacy). Participants were 254 volunteers recruited via convenience sampling. Probabilistic reasoning problems appeared framed within both standard and paranormal contexts. Analysis revealed positive correlations between the Unusual Experience (UnExp) subscale of O-LIFE and paranormal belief measures [RPBS full scale, traditional paranormal beliefs (TPB) and new age philosophy]. Performance on standard problems correlated negatively with UnExp and belief in the paranormal (particularly the TPB dimension of the RPBS). Consideration of specific problem types revealed that perception of randomness associated more strongly with belief in the paranormal than conjunction; both problem types related similarly to UnExp. Structural equation modeling specified that belief in the paranormal mediated the indirect relationship between UnExp and statistical bias. For problems presented in a paranormal context a framing effect occurred. Whilst UnExp correlated positively with conjunction proneness (controlling for perception of randomness), there was no association between UnExp and perception of randomness (controlling for conjunction).

A very low resistance, non-sintered contact system for use on indium phosphide concentrator/shallow junction solar cells

NASA Technical Reports Server (NTRS)

Weizer, Victor G.; Fatemi, Navid S.

1991-01-01

An investigation is made into the possibility of providing low resistance contacts to shallow junction InP solar cells which do not require sintering and which do not cause device degradation even when subjected to extended annealing at elevated temperatures. We show that the addition of In to Au contacts in amounts that exceed the solid solubility limit lowers the as-fabricated (unsintered) contact resistivity (R sub c) to the 10(exp -5) ohm cm(exp 2) range. We next consider the contact system Au/Au2P3, which has been shown to exhibit as-fabricated R sub c values in the 10(exp -6) ohm cm(exp 2) range, but which fails quickly when heated. We show that the substitution of a refractory metal (W, Ta) for Au preserves the low R sub c values while preventing the destructive reactions that would normally take place in this system at high temperatures. We show, finally, that R sub c values in the 10(exp -7) ohm cm(exp 2) range can be achieved without sintering by combining the effects of In or Ga additions to Au contacts with the effects of introducing a thin Au2P3 layer at the metal-InP interface.

Signatures of Exo-Solar Planets in Dust Debris Disks

NASA Technical Reports Server (NTRS)

Ozernoy, Leonid M.; Gorkavyi, Nick N.; Mather, John C.; Taidakova, Tanya A.

1999-01-01

We have developed a new numerical approach to the dynamics of minor bodies and dust particles, which enables us to increase, without using a supercomputer, the number of employed particle positions in each model up to 10(exp 10) - 10(exp 11), a factor of 10(exp 6) - 10(exp 7) higher than existing numerical simulations. We apply this powerful approach to the high-resolution modeling of the structure and emission of circumstellar dust disks, incorporating all relevant physical processes. In this Letter, we examine the resonant structure of a dusty disk induced by the presence of one planet of mass in the range of (5 x 10(exp -5) - 5 x 10(exp -3))M. It is shown that the planet, via resonances and gravitational scattering, produces (i) a central cavity void of dust; (ii) a trailing (sometimes leading) off-center cavity; and (iii) an asymmetric resonant dust belt with one, two, or more clumps. These features can serve as indicators of planet(s) embedded in the circumstellar dust disk and, moreover, can be used to determine the mass of the planet and even some of its orbital parameters. The results of our study reveal a remarkable similarity with various types of highly asymmetric circumstellar disks observed with the JCMT around Epsilon Eridani and Vega.

Towards an International Culture: Gen Y Students and SNS?

ERIC Educational Resources Information Center

Lichy, Jessica

2012-01-01

This article reports the findings of a small-scale investigation into the Internet user behaviour of generation Y (Gen Y) students, with particular reference to social networking sites. The study adds to the literature on cross-cultural Internet user behaviour with specific reference to Gen Y and social networking. It compares how a cohort of…

Farewell to the Chairman - Marine Gen. Peter Pace

Science.gov Websites

oath of office from Marine Gen. Peter Pace, who retired after more than 40 years active duty military wife Lynne after giving the general his retirement certificate. Defense Dept. photo by Cherie A solutions, Marine Gen. Peter Pace said here today as he retired from the Marine Corps after more than 40

Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov. and Middelhovenomyces gen. nov.

USDA-ARS?s Scientific Manuscript database

Three new non-ascosporic, ascomycetous yeast genera are proposed based on their isolation from currently described species and genera. Phylogenetic placement of the genera was determined from analysis of nuclear gene sequences for D1/D2 large subunit rRNA, small subunit rRNA, translation elongation...

Complete cDNAs from Nylanderia cf. pubens (Hymenoptera: Formicidae). --GenBank accession numbers: JF815100-JF815104

USDA-ARS?s Scientific Manuscript database

5 new gene sequences were identified from workers of Caribbean crazy ant, Nylanderia cf. pubens, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are JF815100-JF815104. This information will provide scientists with genetic tools to study the popu...

The Effects of Academic, Behavioral and Organizational Factors on Mathematics Achievement in Secondary Schools

ERIC Educational Resources Information Center

Perez, Miguel S.

2013-01-01

The nation's generation of "Baby Boomers" is now the grandparents of a new generation of students known as "Gen Y" and "Gen Z," respectively. "Gen X," parents of our current students, have the enormous task of raising their children as "digital natives" in a technological-savvy world. The idea of…

Complete cDNAs from Nylanderia sp. nr. pubens (Hymenoptera: Formicidae). GenBank GU980916-GU980928.

USDA-ARS?s Scientific Manuscript database

13 new gene sequences were identified from workers of Rasberry crazy ant, Nylanderia sp.nr. pubens, and submitted to the National Center for Biotechnology Information GenBank. GenBank accession numbers are GU980916-GU980928. This information will provide scientists with genetic tools to study the p...

Discourses in Conflict: The Relationship between Gen Y Pre-Service Teachers, Digital Technologies and Lifelong Learning

ERIC Educational Resources Information Center

Donnison, Sharn

2009-01-01

This paper analyses Gen Y pre-service primary school teachers' conceptualisations of lifelong learning. It is situated within a context of improving the provision and delivery of pre-service teacher education. This paper argues that Gen Y's understanding of lifelong learning has been influenced by their engagements with digital technologies and…

Composting of Sewage Sludge Using Recycled Matured Compost as a Single Bulking Agent

NASA Astrophysics Data System (ADS)

Zhang, Xiangyang; Ren, Jian; Niu, Huasi; Wu, Xingwu

2010-11-01

Pretreatment (bulking agent choice and mixing) is an essential phase of dewatered raw sludge (RS) composting affecting its industrialization significantly. In this paper recycled compost (RC) was chosen as a single bulking agent in the composting experiment instead of other agents such as sawdust, rice straw, MSW, and the mixing machine was developed for mixing of SS and RC. According to the mixing experiment, SS and RC can be mixed uniformly and formed into small particles of 10˜15 mm in diameter, which improved the availability of oxygen during composting. The effect of different volumetric ratios of RS to RC, 1:1 (Exp.1), 1:2 (Exp.2) and 1:4 (Exp.3), on the performance of composting was investigated in detail. Temperature, oxygen consumption rate, organic matter, C/N ratio and moisture content were monitored in each experiment. In despite of low initial C/N of the mixture, intensive fermentation happened in all the experiments. Exp.1 and Exp.2 achieved stability and sanitization, but Exp 1 took more days to accomplish the fermentation. Exp 3 maintained thermophilic temperatures for a shortest time and did not satisfy the necessary sanitation requirements because more RC was recycled. In all experiments, the moisture content of their final composts were too high to be used as bulking agents before extra moisture was reduced. RS: RC = 1:2 (v/v) was the optimum and advisable proportion for the industrialization of sewage sludge composting of, the composting period was about 10 days, and the aeration rate 0.05 m3/(m3ṡmin) was appropriate in this study.

Direct Radiative Effects of Aerosols Over South Asia From Observations and Modeling

NASA Technical Reports Server (NTRS)

Nair, Vijayakumar S.; Babu, S. Suresh; Manoj, M. R.; Moorthy, Krishna K.; Chin, Mian

2016-01-01

Quantitative assessment of the seasonal variations in the direct radiative effect (DRE) of composite aerosols as well as the constituent species over the Indian sub continent has been carried out using a synergy of observations from a dense network of ground based aerosol observatories and modeling based on chemical transport model simulations. Seasonal variation of aerosol constituents depict significant influence of anthropogenic aerosol sources in winter and the dominance of natural sources in spring, even though the aerosol optical depth doesn't change significantly between these two seasons. A significant increase in the surface cooling and atmospheric warming has been observed as season changes from winter DRE(sub SUR) = -28 +/- 12 W m(exp -2) and DRE(sub ATM) = +19.6 +/- 9 W m(exp -2) to spring DRE(sub SUR) = -33.7 +/- 12 W m(exp -2) and DRE(sub ATM) = +27 +/- 9 W m(exp-2). Interestingly, springtime aerosols are more absorptive in nature compared to winter and consequently the aerosol induced diabatic heating of the atmosphere goes as high as approximately 1 K day(exp -1) during spring, especially over eastern India. The atmospheric DRE due to dust aerosols (+14 +/- 7 W m(exp 2) during spring overwhelms that of black carbon DRE (+11.8 +/- 6 W m(exp -2) during winter. The DRE at the top of the atmosphere is mostly governed by the anthropogenic aerosols during all the seasons. The columnar aerosol loading, its anthropogenic fraction and radiative effects shows a steady increase with latitude across Indian mainland leading to a larger aerosol-induced atmospheric warming during spring than in winter.

Characterization of voids formed during liquid impregnation of nonwoven multifilament glass networks as related to composite processing

NASA Technical Reports Server (NTRS)

Mahale, Anant D.; Prudhomme, Robert K.; Rebenfeld, Ludwig

1993-01-01

A technique based on matching the refractive index of an invading liquid to that of a fiber mat was used to study entrapment of air ('voids') that occurs during forced in-plane radial flow into nonwoven multifilament glass networks. The usefulness of this technique is demonstrated in quantifying and mapping the air pockets. Experiments with a series of fluids with surface tensions varying from 28 x 10(exp -3) to 36 x 10(exp -3) N/m, viscosities from 45 x 10(exp -3) to 290 x 10(exp -3) Pa.s, and inlet flow rates from 0.15 x 10(exp -6) to 0.75 x 10(exp -6) m(exp 3)/s, showed that void content is a function of the capillary number characterizing the flow process. A critical value of capillary number, Ca = 2.5 x 10(exp -3), identifies a zone below which void content increases exponentially with decreasing capillary number. Above this critical value, negligible entrapment of voids is observed. Similar experiments carried out on surface treated nonwoven mats spanning a range of equilibrium contact angles from 20 deg to 78 deg showed that there is a critical contact angle above which negligible entrapment is observed. Below this value, there is no apparent effect of contact angle on the void fraction - capillary number relationship described earlier. Studies on the effect of filament wettability, and fluid velocity and viscosity on the size of the entrapment (voids) were also carried out. These indicate that larger sized entrapments which envelop more than one pore are favored by a low capillary number in comparison to smaller, pore level bubbles. Experiments were carried out on deformed mats - imposing high permeability spots at regular intervals on a background of low permeability. The effect of these spatial fluctuations in heterogeneity of the mat on entrapment is currently being studied.

Erosion Data from the MISSE 8 Polymers Experiment After 2 Years of Space Exposure on the International Space Station

NASA Technical Reports Server (NTRS)

de Groh, Kim K.; Banks, Bruce A.; Asmar, Olivia C.; Yi, Grace T.; Mitchell, Gianna G.; Guo, Aobo; Sechkar, Edward A.

2016-01-01

The Polymers Experiment was exposed to the low Earth orbit (LEO) space environment for 2.14 and 2.0 years as part of the Materials International Space Station Experiment 8 (MISSE 8) and the Optical Reflector Materials Experiment-III (ORMatE-III), respectively. The experiment contained 42 samples, which were flown in either ram, wake, or zenith orientations. The primary objective was to determine the effect of solar exposure on the atomic oxygen erosion yield (Ey) of fluoropolymers. This paper provides an overview of the experiment with details on the polymers flown, the characterization techniques used, the atomic oxygen fluence for each exposure orientation, and the LEO Ey results. The Ey values for the fluoropolymers range from 1.45 x 10(exp -25) cm(exp 3)/atom for white Tedlar Registered Trademark? (polyvinyl fluoride with white titanium dioxide pigment) flown in the ram orientation to 6.32 x 10(exp -24) cm(exp 3)/atom for aluminized-Teflon Registered Trademark? fluorinated ethylene propylene (Al-FEP) flown in the zenith orientation. Erosion yield data for FEP flown in ram, wake and zenith orientations are compared, and the Ey was found to be highly dependent on orientation, hence environmental exposure. Teflon FEP had an order of magnitude higher Ey when flown in the zenith direction (6.32 x10(exp -24) cm(exp3)/atom) as compared to the ram direction (2.37 x 10(exp -25) cm(exp 3)/atom). The Ey of FEP was found to increase with a direct correlation to the solar exposure/AO fluence ratio showing the effect of solar radiation and/or heating due to solar exposure on FEP erosion. In addition, back-surface carbon painted FEP (C-FEP) flown in the zenith orientation had a significantly higher Ey than clear FEP or Al-FEP further indicating that heating has a significant impact on the erosion of FEP, particularly in the zenith orientation.

Heterogeneous Reaction of ClONO2(g) + NaCl(s) to Cl2(g) + NaNO3(s)

NASA Technical Reports Server (NTRS)

Timonen, Raimo S.; Chu, Liang T.; Leu, Ming-Taun; Keyser, Leon F.

1994-01-01

The heterogeneous reaction of ClON02 + NaCl yields Cl2 + NaNO3 (eq 1) was investigated over a temperature range 220-300 K in a flow-tube reactor interfaced with a differentially pumped quadrupole mass spectrometer. Partial pressures of ClON02 in the range 10(exp -8) - 10(exp -5) Torr were used. Granule sizes and surface roughness of the NaCl substrates were determined by using a scanning electron microscope, and in separate experiments, surface areas of the substrates were measured by using BET analysis of gas-adsorption isotherms. For dry NaCl substrates, both the decay rates of ClON02 and the growth rates Of C12 were used to obtain reaction probabilities, gamma(sub l) = (4.6 +/- 3.0) x 10(exp -3) at 296 K and (6.7 +/- 3.2) x 10(exp -1) at 225 K, after considering the internal surface area, The error bars represent 1 standard deviation. The Cl2 yield based on the ClONO2 reacted was measured to be 1.0 +/- 0.2. In order to mimic the conditions encountered in the lower stratosphere, the effect of water vapor pressures between 5 x 10(exp -5) and 3 x 10(exp -4) Torr on reaction 1 was also studied. With added H20, reaction probabilities, gamma = (4.1 +/- 2.1) x 10(exp -3) at 296 K and (4.7 +/- 2.9) x 10(exp -3) at 225 K, were obtained. A trace of HOCl, the reaction product from the ClON02 + H20 yield HOCl + HN03 reaction, was observed in addition to the C12 product from reaction 1. The implications of this result for the enhancement of hydrogen chloride in the stratosphere after the El Chichon volcanic eruption and for the marine troposphere are discussed.

Spray measurements of aerothermodynamic effect on disintegrating liquid jets

NASA Technical Reports Server (NTRS)

Ingebo, Robert D.

1991-01-01

An experimental investigation was made to determine the effect of atomizing gas mass flux and temperature on liquid jet breakup in sonic velocity gas flow. Characteristic drop size data were obtained by using the following atomizing gases: nitrogen, argon, and helium to breakup water jets in high velocity gas flow. A scattered light scanning instrument developed at Lewis Research Center was used to measure Sauter mean diameter (SMD). The three gases gave a molecular weight range of 4 to 40 and atomizing gas mass flux and temperature were varied from 6 to 50 g/sq cm and 275-400 K, respectively. The ratio of liquid jet diameter to SMD, D(sub 0)/D(sub 32), was correlated with aerodynamic and liquid-surface force ratios, i.e., the product of the Weber and Reynolds number, We Re, the gas to liquid density ratio, rho(sub g)/rho(sub 1) g and also the molecular scale dimensionless group, rho(sub 1)(Vm exp 3)/ mu(sub 1) g, to give the following expression: D(sub 0)/D(sub 32) = 0.90 x 10(exp -8) x (We Re rho sub g/rho sub 1)exp 0.44 x (rho sub 1 Vm exp 3/mu sub 1 g)exp 0.67 where We Re = ((rho sub g)exp 2(D sub 0)exp 2(V sub C)exp3))/ mu sub 1 sigma, mu sub 1 is liquid viscosity, sigma is surface tension, V sub C is the acoustic gas velocity, V sub m is the RMS velocity of gas molecules, and g is the acceleration of gas molecules due to gravity. Good agreement was obtained with atomization theory for liquid-jet breakup in the regime of aerodynamic stripping. Also, due to its low molecular weight and high acoustic velocity, helium was considerably more effective than nitrogen or argon in producing small-droplet sprays with values of D(sub 32) on the order of 5 microns.

Directly Imaging Damped Ly-Alpha Galaxies at Redshifts Greater Than 2. III: The Star Formation Rates of Neutral Gas Reservoirs at Redshifts of Approximately 2.7

NASA Technical Reports Server (NTRS)

Fumagalli, Michele; OMeara, John M.; Prochaska, J. Xavier; Rafelski, Marc; Kanekar, Nissim

2014-01-01

We present results from a survey designed to probe the star formation properties of 32 damped Ly alpha systems (DLAs) at redshifts of approximately 2.7. By using the "double-DLA" technique that eliminates the glare of the bright background quasars, we directly measure the rest-frame FUV flux from DLAs and their neighbouring galaxies. At the position of the absorbing gas, we place stringent constraints on the unobscured star formation rates (SFRs) of DLAs to 2 sigma limits of psi less than 0.09-0.27 solar mass yr(exp -1), corresponding to SFR surface densities sigma(sub sfr) less than 10(exp -2.6)-10(exp -1.5) solar mass yr(exp -1) kpc(exp -2). The implications of these limits for the star formation law, metal enrichment, and cooling rates of DLAs are examined. By studying the distribution of impact parameters as a function of SFRs for all the galaxies detected around these DLAs, we place new direct constraints on the bright end of the UV luminosity function of DLA hosts. We find that less than or equal to 13% of the hosts have psi greater than or equal to 2 solar mass yr(exp -1) at impact parameters b(sub dla) less than or equal to (psi/solar mass yr(exp -1))(exp 0.8) + 6 kpc, differently from current samples of confirmed DLA galaxies. Our observations also disfavor a scenario in which the majority of DLAs arise from bright LBGs at distances 20 less than or equal to b(sub dla) less than 100 kpc. These new findings corroborate a picture in which DLAs do not originate from highly star forming systems that are coincident with the absorbers, and instead suggest that DLAs are associated with faint, possibly isolated, star-forming galaxies. Potential shortcomings of this scenario and future strategies for further investigation are discussed.

Identification and characterization of an expansin gene AsEXP1 associated with heat tolerance in C3 Agrostis grass species.

PubMed

Xu, Jichen; Tian, Jiang; Belanger, Faith C; Huang, Bingru

2007-01-01

Plant tolerance of heat stress involves various changes at physiological and molecular levels. The objective of this study was to examine the expression of a gene encoding expansin protein in relation to heat tolerance in two C(3) grass species and genotypes differing in heat tolerance. Heat-tolerant, thermal Agrostis scabra, adapted to high temperatures in geothermal areas in Yellowstone National Park, was subjected to 20 degrees C (control) or 40 degrees C (heat stress) for 7 d in a growth chamber. Differential display analysis identified that a gene, AsEXP1, encoding an expansin protein, was strongly up-regulated in leaves exposed to heat stress in thermal A. scabra. Virtual northern hybridization and RT-PCR confirmed that AsEXP1 was a heat-inducible gene in leaves. The expression of AsEXP1 was induced at 1 h of plant exposure to heat stress and reached the highest level of expression at 4 h of treatment. A 1.3 kb full-length cDNA of AsEXP1 was isolated, which encodes a 251 amino acid protein. Two ecotypes of thermal A. scabra and 10 genotypes of Agrostis stolonifera (creeping bentgrass), a widely used turfgrass species in cool climatic regions, varying in the level of heat tolerance, were exposed to 40 degrees C for 7 d to examine the level of AsEXP1 expression in relation to heat tolerance. Genetic variation in heat tolerance was evaluated by measuring cell membrane stability, photochemical efficiency, and leaf growth. RT-PCR analysis revealed that the level of AsEXP1 in different genotypes was positively correlated with the level of heat tolerance in both grass species. The results first identified a heat-related expansin gene in grass species and suggest that AsEXP1 may be useful as a molecular marker to select for heat-tolerant grass germplasm.

X-Ray Emission from the Sun in Its Youth and Old Age

NASA Technical Reports Server (NTRS)

Dorren, J. D.; Gudel, M.; Guinan, E. F.

1995-01-01

We have obtained ROSAT PSPC (Roentgen Satellite Position Sensitive Proportional Counter) pointed observations of two nearby G stars of ages 70 Myr and 9.5 Gyr that are of unique importance as proxies for the Sun at the two extremes of its main-sequence evolutionary lifetime. The younger star, HD 129333 (EK Dra; G0 V), a rapid rotator with a 2.7 day period, is a strong source with an X-ray luminosity L(x)(0.2-2.4 keV) = (7.5-11.5) x 10(exp 29) erg/s. Modeling suggests a two-temperature corona with T(1) = (2.0 +/- 0.3) x 10(exp 6) K and T(2) = (9.7 +/- 0.3) x 10(exp 6) K (formal uncertainties). A continuous emission measure distribution, increasing to higher temperatures and with a cutoff at (20-30) x 10(exp 6) K, yields even better fits to the data. The old star, beta Hyi (HR 98; G2 IV), represents the Sun in the future, near the end of its hydrogen-core burning stage, when it should be rotating more slowly (present P(rot) = 25.4 day) and should have lower levels of activity. The ROSAT measurements yield L(x) = (0.9-3.0) x 10(exp 27) ergs/s and a rather cool, single coronal temperature of T = (1.7 +/- 0.4) x 10(exp 6) K. For comparison, the Sun has L(x) approx. equal to 2 x 10(exp 27) ergs/s and a coronal temperature of about T = 2 x 10(exp 6) K. These stars provide information on the decline of the stellar (and specifically solar) magnetic activity from extreme youth to old age. HD 129333 is also important in that it yields an estimate of the solar soft X-ray flux in the early solar system at the epoch of the terminal stages of planetary accretion.

Mitochondrial complex I protein differs among residual feed intake phenotype in beef cattle.

PubMed

Ramos, M H; Kerley, M S

2013-07-01

Four experiments were performed to determine if residual feed intake (RFI) was related to mitochondrial complex I (CI) protein. For Exp. 1, crossbred Angus steers (initial BW 270 ± 2.0 kg) were fed for a total of 170 d (n = 72). For Exp. 2, crossbred Braunvieh steers (initial BW 280 ± 3.0 kg) were fed for a total of 150 d (n = 50). For Exp. 3, crossbred Braunvieh heifers (initial BW 260 ± 3.0 kg) were fed for a total of 160 d (n = 40). For Exp. 4, crossbred Angus steers (initial BW 290 ± 3.0 kg) were fed for a total of 160 d (n = 40). All cattle in all experiments were fed the same diet. The variable RFI was calculated as the difference between predicted and actual DMI. Predicted DMI was calculated from regressing intake on ADG and metabolic body weight. Blood was collected, lymphocytes were isolated, and antibody used to capture CI. For Exp. 1, 2, and 3, CI quantity was measured using an ELISA commercial kit (Mitosciences, OR). For Exp. 4, CI subunits were separated by gel electrophoresis and bands were analyzed for differences in concentration (absorbance) among RFI phenotypes. For all 4 experiments, there was a significant difference (P < 0.05) between RFI and DMI but no difference (P > 0.05) was reported for ADG and metabolic midweight. For Exp. 1, 2, and 3, CI concentration in mitochondria was greater (P < 0.05) for low RFI compared with other treatments. For Exp. 4, animals with low RFI had a trend (P = 0.07) for greater concentration of Band I (protein S1) than high RFI. Correlation between RFI and Band I was -0.72 (P = 0.04). We concluded that mitochondrial function was at least in part responsible for differences among animals in metabolic efficiency.

Feeding value of whole raw soya beans as a protein supplement for beef cattle consuming low-quality forages.

PubMed

Arelovich, H M; Lagrange, S; Torre, R; Martinez, M F; Laborde, H E

2018-02-01

Experiments (Exp) I and II were conducted to compare raw whole soya beans (WSB), roasted (rWSB) or other protein sources as supplements of low-quality forages fed ad libitum to beef cattle, upon DM intake (DMI), ruminal and blood parameters, and animal performance. Exp I: treatments for wheat straw fed to four ruminally cannulated steers were (i) Control-WS: no supplement; (ii) WSB-WS: whole soya beans; (iii) rWSB-WS: roasted WSB; and (iv) SBM-WS: soybean meal-wheat midds mixture; all fed at 1.4 kg DM/day. Exp II: 12 steers grazed deferred grain sorghum (DS) receiving these treatments: (i) Control-DS: no supplement; (ii) WSB-DS: 1.26 kg DM/day whole soya beans; and (iii) SFM-DS: 1.35 kg DM/day of sunflower meal. In Exp I, WS DMI resulted 47, 52 and 41% greater for WSB-WS, rWSB-WS and SBM-WS, respectively, than Control-WS (p < .05). In Exp II, the DMI of DS was unaffected by supplementation; a substitution of DS by supplement was found for WSB-DS (p < .05); however, total diet and digestible DMI increased with supplementation (p < .05). Rumen pH in Exp I remained unaffected by supplementation, but N-NH 3 as well as blood urea-N in Exp II increased (p < .05). In Exp II, average daily weight gains improved similarly with both supplements compared with Control-DS. Additionally, feed-to-gain ratio decreased (p < .05), being lower for WSB-DS (8.3) vs. SFM-DS (9.9). Roasting effects of WSB as a supplement for low-quality forages were not detected, and all protein sources increased total diet DMI and forage utilization. Only moderate cattle weight gains could be expected for unsupplemented DS. © 2017 Blackwell Verlag GmbH.

Annual Cycles of Multiyear Sea Ice Coverage of the Arctic Ocean: 1999-2003

NASA Technical Reports Server (NTRS)

Kwok, R.

2004-01-01

For the years 1999-2003, we estimate the time-varying perennial ice zone (PIZ) coverage and construct the annual cycles of multiyear (MY, including second year) ice coverage of the Arctic Ocean using QuikSCAT backscatter, MY fractions from RADARSAT, and the record of ice export from satellite passive microwave observations. An area balance approach extends the winter MY coverage from QuikSCAT to the remainder of the year. From these estimates, the coverage of MY ice at the beginning of each year is 3774 x 10(exp 3) sq km (2000), 3896 x 10(exp 3) sq km (2001), 4475 x 10(exp 3) sq km (2002), and 4122 x 10(exp 3) sq km (2003). Uncertainties in coverage are approx.150 x 10(exp 3) sq km. In the mean, on 1 January, MY ice covers approx.60% of the Arctic Ocean. Ice export reduces this coverage to approx.55% by 1 May. From the multiple annual cycles, the area of first-year (FY) ice that survives the intervening summers are 1192 x 10(exp 3) sq km (2000), 1509 x 10(exp 3) sq km (2001), and 582 x 10(exp 3) sq km (2002). In order for the MY coverage to remain constant from year to year, these replenishment areas must balance the overall area export and melt during the summer. The effect of the record minimum in Arctic sea ice area during the summer of 2002 is seen in the lowest area of surviving FY ice of the three summers. In addition to the spatial coverage, the location of the PIZ is important. One consequence of the unusual location of the PIZ at the end of the summer of 2002 is the preconditioning for enhanced export of MY ice into the Barents and Kara seas. Differences between the minimums in summer sea ice coverage from our estimates and passive microwave observations are discussed.

High-energy astrophysics: A theoretical analysis of thermal radiation from neutron stars

NASA Technical Reports Server (NTRS)

Applegate, James H.

1994-01-01

The unambiguous detection of thermal radiation from the surface of a cooling neutron star was one of the most anxiously awaited results in neutron star physics. This particular Holy Grail was found by Halpern and Holt, who used ROSAT to detect pulsed X-rays from the gamma-ray source Geminga and demonstrate that it was a neutron star, probably a radio pulsar beamed away from us. At an age of approximately 3.4 x 10(exp 5) years, Geminga is in the photon cooling era. Its surface temperature of 5.2 x 10(exp 5) K can be explained within the contexts of both the slow and fast cooling scenarios. In the slow cooling scenario, the surface temperature is too high unless the specific heat of the interior is reduced by extensive baryon pairing. In the fast cooling scenario, the surface temperature will be much too low unless the fast neutrino cooling is shut off by baryon pairing. Two other pulsars, PSR 0656+14 and PSR 1055-52, have also been detected in thermal X-rays by ROSAT. They are also in the photon cooling era. All of this research's neutron star cooling models to date have used the unmagnetized effective temperature-interior temperature relation for the outer boundary condition. Models are being improved by using published magnetic envelope calculations and assumed geometried for the surface magnetic field to determine local interior temperature-emitted flux relations for the surface of the star.

Spectral Analyses and Radiation Exposures from Several Ground-Level Enhancement (GLE) Solar Proton Events: A Comparison of Methodologies

NASA Technical Reports Server (NTRS)

Atwell, William; Tylka, Allan; Dietrich, William; Badavi, Francis; Rojdev, Kristina

2011-01-01

Several methods for analyzing the particle spectra from extremely large solar proton events, called Ground-Level Enhancements (GLEs), have been developed and utilized by the scientific community to describe the solar proton energy spectra and have been further applied to ascertain the radiation exposures to humans and radio-sensitive systems, namely electronics. In this paper 12 GLEs dating back to 1956 are discussed, and the three methods for describing the solar proton energy spectra are reviewed. The three spectral fitting methodologies are EXP [an exponential in proton rigidity (R)], WEIB [Weibull fit: an exponential in proton energy], and the Band function (BAND) [a double power law in proton rigidity]. The EXP and WEIB methods use low energy (MeV) GLE solar proton data and make extrapolations out to approx.1 GeV. On the other hand, the BAND method utilizes low- and medium-energy satellite solar proton data combined with high-energy solar proton data deduced from high-latitude neutron monitoring stations. Thus, the BAND method completely describes the entire proton energy spectrum based on actual solar proton observations out to 10 GeV. Using the differential spectra produced from each of the 12 selected GLEs for each of the three methods, radiation exposures are presented and discussed in detail. These radiation exposures are then compared with the current 30-day and annual crew exposure limits and the radiation effects to electronics.

An Adjuvanted Herpes Simplex Virus 2 Subunit Vaccine Elicits a T Cell Response in Mice and Is an Effective Therapeutic Vaccine in Guinea Pigs

PubMed Central

Skoberne, Mojca; Cardin, Rhonda; Lee, Alexander; Kazimirova, Ana; Zielinski, Veronica; Garvie, Danielle; Lundberg, Amy; Larson, Shane; Bravo, Fernando J.; Bernstein, David I.; Flechtner, Jessica B.

2013-01-01

Immunotherapeutic herpes simplex virus 2 (HSV-2) vaccine efficacy depends upon the promotion of antigen-specific immune responses that inhibit reactivation or reactivated virus, thus controlling both recurrent lesions and viral shedding. In the present study, a candidate subunit vaccine, GEN-003/MM-2, was evaluated for its ability to induce a broad-spectrum immune response in mice and therapeutic efficacy in HSV-2-infected guinea pigs. GEN-003 is comprised of HSV-2 glycoprotein D2 (gD2ΔTMR340-363) and a truncated form of infected cell polypeptide 4 (ICP4383-766), formulated with Matrix M-2 (MM-2) adjuvant (GEN-003/MM-2). In addition to eliciting humoral immune responses, CD4+ and CD8+ T cells characterized by the secretion of multiple cytokines and cytolytic antigen-specific T cell responses that were able to be recalled at least 44 days after the last immunization were induced in immunized mice. Furthermore, vaccination with either GEN-003 or GEN-003/MM-2 led to significant reductions in both the prevalence and severity of lesions in HSV-2-infected guinea pigs compared to those of phosphate-buffered saline (PBS) control-vaccinated animals. While vaccination with MM-2 adjuvant alone decreased recurrent disease symptoms compared to the PBS control group, the difference was not statistically significant. Importantly, the frequency of recurrent viral shedding was considerably reduced in GEN-003/MM-2-vaccinated animals but not in GEN-003- or MM-2-vaccinated animals. These findings suggest a possible role for immunotherapeutic GEN-003/MM-2 vaccination as a viable alternative to chronic antiviral drugs in the treatment and control of genital herpes disease. PMID:23365421

A high-precision rule-based extraction system for expanding geospatial metadata in GenBank records

PubMed Central

Weissenbacher, Davy; Rivera, Robert; Beard, Rachel; Firago, Mari; Wallstrom, Garrick; Scotch, Matthew; Gonzalez, Graciela

2016-01-01

Objective The metadata reflecting the location of the infected host (LOIH) of virus sequences in GenBank often lacks specificity. This work seeks to enhance this metadata by extracting more specific geographic information from related full-text articles and mapping them to their latitude/longitudes using knowledge derived from external geographical databases. Materials and Methods We developed a rule-based information extraction framework for linking GenBank records to the latitude/longitudes of the LOIH. Our system first extracts existing geospatial metadata from GenBank records and attempts to improve it by seeking additional, relevant geographic information from text and tables in related full-text PubMed Central articles. The final extracted locations of the records, based on data assimilated from these sources, are then disambiguated and mapped to their respective geo-coordinates. We evaluated our approach on a manually annotated dataset comprising of 5728 GenBank records for the influenza A virus. Results We found the precision, recall, and f-measure of our system for linking GenBank records to the latitude/longitudes of their LOIH to be 0.832, 0.967, and 0.894, respectively. Discussion Our system had a high level of accuracy for linking GenBank records to the geo-coordinates of the LOIH. However, it can be further improved by expanding our database of geospatial data, incorporating spell correction, and enhancing the rules used for extraction. Conclusion Our system performs reasonably well for linking GenBank records for the influenza A virus to the geo-coordinates of their LOIH based on record metadata and information extracted from related full-text articles. PMID:26911818

A high-precision rule-based extraction system for expanding geospatial metadata in GenBank records.

PubMed

Tahsin, Tasnia; Weissenbacher, Davy; Rivera, Robert; Beard, Rachel; Firago, Mari; Wallstrom, Garrick; Scotch, Matthew; Gonzalez, Graciela

2016-09-01

The metadata reflecting the location of the infected host (LOIH) of virus sequences in GenBank often lacks specificity. This work seeks to enhance this metadata by extracting more specific geographic information from related full-text articles and mapping them to their latitude/longitudes using knowledge derived from external geographical databases. We developed a rule-based information extraction framework for linking GenBank records to the latitude/longitudes of the LOIH. Our system first extracts existing geospatial metadata from GenBank records and attempts to improve it by seeking additional, relevant geographic information from text and tables in related full-text PubMed Central articles. The final extracted locations of the records, based on data assimilated from these sources, are then disambiguated and mapped to their respective geo-coordinates. We evaluated our approach on a manually annotated dataset comprising of 5728 GenBank records for the influenza A virus. We found the precision, recall, and f-measure of our system for linking GenBank records to the latitude/longitudes of their LOIH to be 0.832, 0.967, and 0.894, respectively. Our system had a high level of accuracy for linking GenBank records to the geo-coordinates of the LOIH. However, it can be further improved by expanding our database of geospatial data, incorporating spell correction, and enhancing the rules used for extraction. Our system performs reasonably well for linking GenBank records for the influenza A virus to the geo-coordinates of their LOIH based on record metadata and information extracted from related full-text articles. © The Author 2016. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Therapeutic Vaccine for Genital Herpes Simplex Virus-2 Infection: Findings From a Randomized Trial.

PubMed

Bernstein, David I; Wald, Anna; Warren, Terri; Fife, Kenneth; Tyring, Stephen; Lee, Patricia; Van Wagoner, Nick; Magaret, Amalia; Flechtner, Jessica B; Tasker, Sybil; Chan, Jason; Morris, Amy; Hetherington, Seth

2017-03-15

Genital herpes simplex virus type 2 (HSV-2) infection causes recurrent lesions and frequent viral shedding. GEN-003 is a candidate therapeutic vaccine containing HSV-2 gD2∆TMR and ICP4.2, and Matrix-M2 adjuvant. Persons with genital herpes were randomized into 3 dose cohorts to receive 3 intramuscular doses 21 days apart of 10 µg, 30 µg, or 100 µg of GEN-003, antigens without adjuvant, or placebo. Participants obtained genital swab specimens twice daily for HSV-2 detection and monitored genital lesions for 28-day periods at baseline and at intervals after the last dose. One hundred and thirty-four persons received all 3 doses. Reactogenicity was associated with adjuvant but not with antigen dose or dose number. No serious adverse events were attributed to GEN-003. Compared with baseline, genital HSV-2 shedding rates immediately after dosing were reduced with GEN-003 (from 13.4% to 6.4% for 30 μg [P < .001] and from 15.0% to 10.3% for 100 µg [P < .001]). Lesion rates were also significantly (P < .01) reduced immediately following immunization with 30 µg or 100 µg of GEN-003. GEN-003 elicited increases in antigen binding, virus neutralizing antibody, and T-cell responses. GEN-003 had an acceptable safety profile and stimulated humoral and cellular immune responses. GEN-003 at doses of 30 µg and 100 µg reduced genital HSV shedding and lesion rates. NCT01667341 (funded by Genocea). © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

2009/2010 Eurasian Cold Winter and Loss of Arctic Sea-ice over Barents/Kara Sea

NASA Astrophysics Data System (ADS)

Shim, T.; Kim, B.; Kim, S.

2012-12-01

In 2009/2010 winter, a few extreme cold events and heavy snowfall occurred over central North America, north western Europe, and East Asia exerting a severe social and economic impacts. In this study, we performed modeling experiments to examine the role of substantially reduced Arctic sea-ice over Barents/Kara Sea on the 2009/2010 cold winters. Although several previous studies investigated cause of the extreme events and emphasized the large snow-covered area over Siberia in autumn 2009, we note that the area extent of Arctic sea-ice over Barents/Kara sea in autumn 2009 was anomalously low and the possible impact from Arctic for the extreme cold events has not been presented. To investigate the influence from the Arctic, we designed three model runs using Community Atmosphere Model Version 3 (CAM3). Each simulation differs by the prescribed surface boundary conditions: (a) CTRL - climatological seasonal cycle of sea surface temperature (SST) and sea-ice concentration (SIC) are prescribed everywhere, (b) EXP_65N - SST and SIC inside the Arctic circle (north of 65°N) are replaced by 2009/2010 values. Elsewhere, the climatology is used, (c) EXP_BK - Same with (b) except that SIC and SST are fixed only over Barents/Kara Sea where the sea-ice area dropped significantly in 2009/2010 winter. Model results from EXP_65N and EXP_BK commonly showed a large increase of air temperature in the lower troposphere where Arctic sea-ice showed a large reduction. Also, compared with the observation, model successfully captured thickened geopotential height in the Arctic and showed downstream wave propagation toward midlatitude. From the analysis, we reveal that this large dipolar Arctic-midlatitude teleconnection pattern in the upper troposphere easily propagate upward and played a role in the weakening of polar vortex. This is also confirmed in the observation. However, the timing of excitation of upward propagating wave in EXP_65N and EXP_BK were different and thus the timing of weakening of polar vortex also differs in each experiment. Unlike with our expectation, both EXP_65N and EXP_BK did not capture the abrupt increase of snow-cover in the observation over Siberian region in autumn 2009. Therefore, given the successful reproduction of key observed features of cold winter 2009/2010 by EXP_65N and EXP_BK, we conclude that Arctic sea-ice in autumn 2009 played a key role for the subsequent development of cold winter 2009/2010 and the role was largely independent with the autumn snow-cover.

Impact of oral meloxicam administration before and after band castration on feedlot performance and behavioral response in weanling beef bulls.

PubMed

Repenning, P E; Ahola, J K; Callan, R J; French, J T; Giles, R L; Bigler, B J; Coetzee, J F; Wulf, L W; Peel, R K; Whittier, J C; Fox, J T; Engle, T E

2013-10-01

Two experiments evaluated the effects of band castration and oral administration of an analgesic in association with castration on performance and behavioral and physiological responses in yearling beef bulls. In Exp. 1 Angus and Charolais-crossbred bull calves (n = 127; 309.8 ± 59.04 kg BW) and in Exp. 2 Hereford, Angus, and Hereford × Angus crossbred bulls (n = 30; 300.8 ± 4.96 kg BW) were stratified by BW and randomly assigned to 1 of 3 treatments: 1) band castration (BAND), 2) band castration with oral administration of meloxicam (BAND-MEL), and 3) sham castration (SHAM). The BAND and SHAM procedures were completed on d 0. The SHAM treatment consisted of all animal manipulations associated with band castration without band application. Meloxicam was administered on d -1, 0, and 1 (1.0, 0.5, and 0.5 mg/kg, respectively) via an oral bolus. Body weight and a subjective chute score (CS) were collected on d -1, 0, 1, 7, 14, and 21 (d 28 Exp. 1 only). In Exp. 2, jugular blood samples were collected immediately before castration and 24 h postcastration for substance P (SP) analysis. In Exp. 2, video documentation on d 0 was used to determine range of vertical head motion (DIST) on a subset of animals during treatment administration. In both experiments, ADG was similar (P ≥ 0.50) between BAND and BAND-MEL, but ADG in SHAM cattle was greater (P < 0.001) and tended (P = 0.07) to be greater than castrates in Exp. 1 and 2, respectively. In Exp. 1, CS did not differ (P ≥ 0.26) between BAND and BAND-MEL on any day, but castrates exhibited less desirable CS on d 1 and 28 than SHAM cattle. In Exp. 2, CS was not affected (P ≥ 0.41) by castration or the presence of meloxicam. In Exp. 2, DIST did not differ (P = 0.57) between BAND and BAND-MEL, but when pooled, castrates exhibited greater (P = 0.04) DIST than SHAM. In Exp. 2, plasma SP concentrations were similar between BAND and BAND-MEL (P = 0.81) and between castrates vs. sham cattle (P = 0.67). Results indicate no impact of meloxicam administration on performance or behavioral and physiological responses to band castration. However, there was a negative impact of castration on ADG and DIST.

On the new monotypic wolf spider genus Ovia gen. nov. (Araneae: Lycosidae, Lycosinae).

PubMed

Sankaran, Pradeep M; Malamel, Jobi J; Sebastian, Pothalil A

2017-01-17

A new monotypic wolf spider genus, Ovia gen. nov. is proposed to accommodate a misplaced species: Pardosa procurva Yu & Song, 1988. Ovia procurva comb. nov. is redescribed, illustrated and designated as the type species for the genus. The subfamily placement of the new genus is discussed and it is considered as a member of Lycosinae Sundevall, 1833 and possibly closely related to Alopecosa Simon, 1885. The presence of an apical process (spur) on the median apophysis is proposed as the putative synapomorphy of Ovia gen. nov. The possible sister-taxon relationship of Ovia gen. nov. with Alopecosa is discussed and evidence on the occurrence of sexual dimorphism and mating plug within the genus are presented. Ovia gen. nov. is assumed to be of Holarctic origin, from which it has migrated to the Indomalayan region. Additionally, a current distribution map for the genus is provided.

Rare cancers in children - The EXPeRT Initiative: a report from the European Cooperative Study Group on Pediatric Rare Tumors.

PubMed

Bisogno, G; Ferrari, A; Bien, E; Brecht, I B; Brennan, B; Cecchetto, G; Godzinski, J; Orbach, D; Reguerre, Y; Stachowicz-Stencel, T; Schneider, D T

2012-10-01

The low incidence and the heterogeneity of very rare tumors (VRTs) demand for international cooperation. In 2008, EXPeRT (European Cooperative Study Group for Pediatric Rare Tumors) was founded by national groups from Italy, France, United Kingdom, Poland and Germany. The first aims of EXPeRT were to agree on a uniform definition of VRTs and to develop the currently most relevant scientific questions. Current initiatives include international data exchange, retrospective and prospective studies of specific entities, and the development of harmonized and internationally recognized guidelines. Moreover, EXPeRT established a network for expert consultation to assist in clinical decision in VRTs. © Georg Thieme Verlag KG Stuttgart · New York.

The interaction with the lower ionosphere of electromagnetic pulses from lightning: Excitation of optical emissions

NASA Technical Reports Server (NTRS)

Taranenko, Y. N.; Inan, U. S.; Bell, T. F.

1993-01-01

A self consistent and fully kinetic simulation of the interaction of lightning radiated electromagnetic (EM) pulses with the nighttime lower ionosphere indicates that optical emissions observable with conventional instruments would be excited. For example, emissions of the 1st and 2nd positive bands of N2 occur at rates reaching 7 x 10(exp 7) and 10(exp 7) cu cm/s respectively at 92 km altitude for a lightning discharge with an electric field E(sub 100) = 20 V/m (normalized to a 100 km distance). The maximum height integrated intensities of these emissions are 4 x 10(exp 7) and 6 x 10(exp 6) R respectively, lasting for approx. 50 micrometers.

"We Understood It More 'Cause We Were Doin' It Ourself": Students' Self-Described Connections between Participation and Learning.

ERIC Educational Resources Information Center

Christie, Mary Ann

The experiences and responses of high school biology students using the GenScope computer program are described. GenScope represents genetic concepts in a linked multilevel fashion to teach students to think like scientists. Many GenScope problems use a fictitious dragon species to illustrate genetics. Students can manipulate the dragons' genes to…

75 FR 5780 - Green Borders Geothermal, LLC, Complainant, v. Terra-Gen Dixie Valley, LLC, Respondent; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-04

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. EL10-36-000] Green Borders Geothermal, LLC, Complainant, v. Terra-Gen Dixie Valley, LLC, Respondent; Notice of Complaint January 28, 2010. Take notice that on January 25, 2010, Green Borders Geothermal, LLC (Green Borders) filed a formal complaint against Terra-Gen Dixie Valle...

Supplementation of OmniGen-AF alters the metabolic response to a glucose tolerance test in beef heifers

USDA-ARS?s Scientific Manuscript database

This study was designed to determine whether feeding OmniGen-AF to feedlot heifers would alter metabolic profiles in response to a glucose tolerance test. Heifer calves (n=184; 216±1 kg) were allocated into 2 treatment diets: 1) Control, fed a standard receiving ration, and 2) OmniGen-AF (OG), fed t...

DD 21A-A Capable, Affordable, Modular 21st Century Destroyer

DTIC Science & Technology

1993-12-01

1 1.000 2 0.OOOOE+00 3 0.OOOOE+00 4 1.000 5 1.000 ARRANGEMENT OPERATION SEP SS GEN OP ARRAY = ( 2X 1) 1 2.000 2 2.000 ARRANGEMENT CG MACHY KG IND... GEN SIZE IND = GIVEN SEP SS GEN KW = 2000.00 KW SS ENGINES SS ENG SELECT IND = GIVEN SS ENG MODEL IND = DDA-501-K17 SS ENG TYPE IND = GT SS ENG SIZE...1733.6 PROPELLERS: 2 - CP - 17.0 FT DIA AREA SUMMARY - FT2 SEP GEN : 3 CT 0 2000.0 KW HULL AREA - 55529.2 SUPERSTRUCTURE AREA - 21232.1 24 HR LOAD 1858.1

GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update

PubMed Central

Peakall, Rod; Smouse, Peter E.

2012-01-01

Summary: GenAlEx: Genetic Analysis in Excel is a cross-platform package for population genetic analyses that runs within Microsoft Excel. GenAlEx offers analysis of diploid codominant, haploid and binary genetic loci and DNA sequences. Both frequency-based (F-statistics, heterozygosity, HWE, population assignment, relatedness) and distance-based (AMOVA, PCoA, Mantel tests, multivariate spatial autocorrelation) analyses are provided. New features include calculation of new estimators of population structure: G′ST, G′′ST, Jost’s Dest and F′ST through AMOVA, Shannon Information analysis, linkage disequilibrium analysis for biallelic data and novel heterogeneity tests for spatial autocorrelation analysis. Export to more than 30 other data formats is provided. Teaching tutorials and expanded step-by-step output options are included. The comprehensive guide has been fully revised. Availability and implementation: GenAlEx is written in VBA and provided as a Microsoft Excel Add-in (compatible with Excel 2003, 2007, 2010 on PC; Excel 2004, 2011 on Macintosh). GenAlEx, and supporting documentation and tutorials are freely available at: http://biology.anu.edu.au/GenAlEx. Contact: rod.peakall@anu.edu.au PMID:22820204

Genistein improves spatial learning and memory in male rats with elevated glucose level during memory consolidation.

PubMed

Kohara, Yumi; Kawaguchi, Shinichiro; Kuwahara, Rika; Uchida, Yutaro; Oku, Yushi; Yamashita, Kimihiro

2015-03-01

Cognitive dysfunction due to higher blood glucose level has been reported previously. Genistein (GEN) is a phytoestrogen that we hypothesized might lead to improved memory, despite elevated blood glucose levels at the time of memory consolidation. To investigate this hypothesis, we compared the effects of orally administered GEN on the central nervous system in normal versus glucose-loaded adult male rats. A battery of behavioral assessments was carried out. In the MAZE test, which measured spatial learning and memory, the time of normal rats was shortened by GEN treatment compared to the vehicle group, but only in the early stages of testing. In the glucose-loaded group, GEN treatment improved performance as mazes were advanced. In the open-field test, GEN treatment delayed habituation to the new environment in normal rats, and increased the exploratory behaviors of glucose-loaded rats. There were no significant differences observed for emotionality or fear-motivated learning and memory. Together, these results indicate that GEN treatment improved spatial learning and memory only in the early stages of testing in the normal state, but improved spatial learning and memory when glucose levels increased during memory consolidation. Copyright © 2014 Elsevier Inc. All rights reserved.

The natural history of cutaneous propionibacteria, and reclassification of selected species within the genus Propionibacterium to the proposed novel genera Acidipropionibacterium gen. nov., Cutibacterium gen. nov. and Pseudopropionibacterium gen. nov.

PubMed

Scholz, Christian F P; Kilian, Mogens

2016-11-01

The genus Propionibacterium in the family Propionibacteriaceaeconsists of species of various habitats, including mature cheese, cattle rumen and human skin. Traditionally, these species have been grouped as either classical or cutaneous propionibacteria based on characteristic phenotypes and source of isolation. To re-evaluate the taxonomy of the family and to elucidate the interspecies relatedness we compared 162 public whole-genome sequences of strains representing species of the family Propionibacteriaceae. We found substantial discrepancies between the phylogenetic signals of 16S rRNA gene sequence analysis and our high-resolution core-genome analysis. To accommodate these discrepancies, and to address the long-standing issue of the taxonomically problematic Propionibacterium propionicum, we propose three novel genera, Acidipropionibacterium gen. nov., Cutibacterium gen. nov. and Pseudopropionibacterium gen. nov., and an amended description of the genus Propionibacterium. Furthermore, our genome-based analyses support the amounting evidence that the subdivision of Propionibacterium freudenreichii into subspecies is not warranted. Our proposals are supported by phylogenetic analyses, DNA G+C content, peptidoglycan composition and patterns of the gene losses and acquisitions in the cutaneous propionibacteria during their adaptation to the human host.

GEN1 from a thermophilic fungus is functionally closely similar to non-eukaryotic junction-resolving enzymes.

PubMed

Freeman, Alasdair D J; Liu, Yijin; Déclais, Anne-Cécile; Gartner, Anton; Lilley, David M J

2014-12-12

Processing of Holliday junctions is essential in recombination. We have identified the gene for the junction-resolving enzyme GEN1 from the thermophilic fungus Chaetomium thermophilum and expressed the N-terminal 487-amino-acid section. The protein is a nuclease that is highly selective for four-way DNA junctions, cleaving 1nt 3' to the point of strand exchange on two strands symmetrically disposed about a diagonal axis. CtGEN1 binds to DNA junctions as a discrete homodimer with nanomolar affinity. Analysis of the kinetics of cruciform cleavage shows that cleavage of the second strand occurs an order of magnitude faster than the first cleavage so as to generate a productive resolution event. All these properties are closely similar to those described for bacterial, phage and mitochondrial junction-resolving enzymes. CtGEN1 is also similar in properties to the human enzyme but lacks the problems with aggregation that currently prevent detailed analysis of the latter protein. CtGEN1 is thus an excellent enzyme with which to engage in biophysical and structural analysis of eukaryotic GEN1. Copyright © 2014. Published by Elsevier Ltd.

Synthesis and characterization of drug loaded albumin mesospheres for intratumoral chemotherapy

NASA Astrophysics Data System (ADS)

Freeman, Shema Taian

Conventional chemotherapy is problematic due to toxic complications. Intratumoral (IT) drug delivery, offers a new, less toxic, potentially more effective treatment concept. The objectives of this research encompassed (1) an investigation of the synthesis of BSA mesospheres (MS) employing genipin (GEN) as a novel crosslinking agent, (2) comparison with glutaraldehyde (GTA) crosslinked mesosphere, (3) a study of process parameters to define conditions for the synthesis of 1-10microm drug loaded mesospheres, and (4) investigation of the drug delivery properties of such mesospheres for IT chemotherapy. Smooth, spherical BSA-MS, crosslinked with glutaraldehyde and genipin, were prepared in a dry particle size range of 1microm to 10microm. It was shown that increasing dispersion stirring rate, crosslinking time and GEN/BSA ratio led to a decrease in particle size and a narrower particle distribution. It was also shown that increasing crosslinking time, GEN/BSA ratio, BSA concentrations, GEN concentration slowed enzymatic degradation. Post-loading and in situ drug loading methods were studied for the incorporation of cyclophosphamide and cisplatin into mesospheres. Maximum post loading of cisplatin was 3.2% (w/w) and 2.6% (w/w) with GEN and with GTA crosslinking. For cyclophosphamide 8.2% (w/w) and 7.1% (w/w) loading was achieved with GEN and GTA respectively. In situ drug loaded MS genipin and glutaraldehyde crosslinked mesospheres were also synthesized with 1.8% (w/w) cisplatin (using GEN) and 1.2% (w/w) (using GTA). Maximum loading of 13.3% (w/w) was achieved for cyclophosphamide in genipin crosslinked mesospheres. The cytotoxicity of in situ loaded genipin and glutaraldehyde crosslinked cisplatin mesospheres was evaluated using a murine Lewis lung model. Both genipin and glutaraldehyde crosslinked BSA-cisplatin mesospheres proved to be cytotoxic during a 48 hour test. Ultimately a standard set of processing parameters (BSA concentration, CAB concentration, GEN concentration, GEN/BSA ratio, stabilization stirring rate and crosslinking time) were defined to produce both GEN and GTA crosslinked cisplatin and cyclophosphamide BSA mesospheres. In vitro analysis confirmed the utility of mesosphere bound drug. In several related studies, (1) IT delivered dispersions of mitoxantrone loaded albumin microspheres were shown to afford an effective treatment, with significantly prolonging animal survival and (2) genipin and gadolinium crosslinked MS were prepared from HA and BSA/HA.

A Climatology of Midlatitude Continental Clouds from the ARM SGP Site. Part II; Cloud Fraction and Surface Radiative Forcing

NASA Technical Reports Server (NTRS)

Xi, B.; Minnis, P.

2006-01-01

Data collected at the Department of Energy Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) Central Facility (SCF) are analyzed to determine the monthly and hourly variations of cloud fraction and radiative forcing between January 1997 and December 2002. Cloud fractions are estimated for total cloud cover and for single-layered low (0-3 km), middle (3-6 km), and high clouds (more than 6 km) using ARM SCG ground-based paired lidar-radar measurements. Shortwave (SW) and longwave (LW) fluxes are derived from up- and down-looking standard precision spectral pyranometers and precision infrared radiometer measurements with uncertainties of approximately 10 Wm(exp -2). The annual averages of total, and single-layered low, middle and high cloud fractions are 0.49, 0.11, 0.03, and 0.17, respectively. Both total and low cloud amounts peak during January and February and reach a minimum during July and August, high clouds occur more frequently than other types of clouds with a peak in summer. The average annual downwelling surface SW fluxes for total and low clouds (151 and 138 Wm(exp-2), respectively) are less than those under middle and high clouds (188 and 201 Wm(exp -2), respectively), but the downwelling LW fluxes (349 and 356 Wm(exp -2)) underneath total and low clouds are greater than those from middle and high clouds (337 and 333 Wm(exp -2)). Low clouds produce the largest LW warming (55 Wm(exp -2) and SW cooling (-91 Wm(exp -2)) effects with maximum and minimum absolute values in spring and summer, respectively. High clouds have the smallest LW warming (17 Wm(exp -2)) and SW cooling (-37 Wm(exp -2)) effects at the surface. All-sky SW CRF decreases and LW CRF increases with increasing cloud fraction with mean slopes of -0.984 and 0.616 Wm(exp -2)%(exp -1), respectively. Over the entire diurnal cycle, clouds deplete the amount of surface insolation more than they add to the downwelling LW flux. The calculated CRFs do not appear to be significantly affected by uncertainties in data sampling and clear-sky screening. Traditionally, cloud radiative forcing includes, not only the radiative impact of the hydrometeors, but also the changes in the environment. Taken together over the ARM SCF, changes in humidity and surface albedo between clear and cloudy conditions offset approximately 20% of the NET radiative forcing caused by the cloud hydrometeors alone. Variations in water vapor, on average, account for 10% and 83% of the SW and LW CRFs, respectively, in total cloud cover conditions. The error analysis further reveals that the cloud hydrometeors dominate the SW CRF, while water vapor changes are most important for LW flux changes in cloudy skies. Similar studies over other locales are encouraged where water and surface albedo changes from clear to cloudy conditions may be much different than observed over the ARM SCF.

Indirubin, a component of Ban-Lan-Gen, activates CYP3A4 gene transcription through the human pregnane X receptor.

PubMed

Kumagai, Takeshi; Aratsu, Yusuke; Sugawara, Ryosuke; Sasaki, Takamitsu; Miyairi, Shinichi; Nagata, Kiyoshi

2016-04-01

Ban-Lan-Gen is the common name for the dried roots of indigo plants, including Polygonum tinctorium, Isatis indigotica, Isatis tinctoria, and Strobilanthes cusia. Ban-Lan-Gen is frequently used as an anti-inflammatory and an anti-viral for the treatment of hepatitis, influenza, and various types of inflammation. One of the cytochrome P450 (CYP) enzymes, CYP3A4, is responsible for the metabolism of a wide variety of xenobiotics, including an estimated 60% of all clinically used drugs. In this study, we investigated the effect of Ban-Lan-Gen on the transcriptional activation of the CYP3A4 gene. Ban-Lan-Gen extract increased CYP3A4 gene reporter activity in a dose-dependent manner. Indirubin, one of the biologically active ingredients in the Ban-Lan-Gen, also dose-dependently increased CYP3A4 gene reporter activity. Expression of short hairpin RNA for the human pregnane X receptor (hPXR-shRNA) inhibited CYP3A4 gene reporter activity, and overexpression of human PXR increased indirubin- and rifampicin-induced CYP3A4 gene reporter activity. Furthermore, indirubin induced CYP3A4 mRNA expression in HepG2 cells. Taken together, these results indicate that indirubin, a component of Ban-Lan-Gen, activated CYP3A4 gene transcription through the activation of the human PXR. Copyright © 2016. Published by Elsevier Ltd.

A randomized trial to determine the diagnostic accuracy of conventional vs. jumbo forceps biopsy of gastric epithelial neoplasias before endoscopic submucosal dissection; open-label study.

PubMed

Jeon, Hyo Keun; Ryu, Ho Yoel; Cho, Mee Yon; Kim, Hyun-Soo; Kim, Jae Woo; Park, Hong Jun; Kim, Moon Young; Baik, Soon Koo; Kwon, Sang Ok; Park, Su Yeon; Won, Sung Ho

2014-10-01

Larger biopsy specimens or increasing the number of biopsies may improve the diagnostic accuracy of gastric epithelial neoplasia (GEN). The aims of this study was to compare the diagnostic accuracies between conventional and jumbo forceps biopsy of GEN before endoscopic submucosal dissection (ESD) and to confirm that increasing the number of biopsies is useful for the diagnosis of GEN. The concordance rate between EFB and ESD specimens was not significantly different between the two groups [83.1 % (54/65) in JG vs. 79.1 % (53/67) in CG]. On multivariate analyses, two or four EFBs significantly increased the cumulating concordance rate [coefficients; twice: 5.1 (P = 0.01), four times: 5.9 (P = 0.02)]. But, the concordance rate was decreased in high grade dysplasia (coefficient -40.32, P = 0.006). One hundred and sixty GENs from 148 patients were randomized into two groups and finally 67 GENs in 61 patients and 65 GENs in 63 patients were allocated to the conventional group (CG) or jumbo group (JG), respectively. Four endoscopic forceps biopsy (EFB) specimens were obtained from each lesion with conventional (6.8 mm) forceps or jumbo (8 mm) forceps. The histological concordance rate between 4 EFB specimens and ESD specimens was investigated in the two groups. Before ESD, the diagnostic accuracy of GENs was significantly increased not by the use of jumbo forceps biopsy but by increasing the number of biopsies.

CO2 exchange in the Hudson Bay lowlands: Community characteristics and multispectral reflectance properties

NASA Technical Reports Server (NTRS)

Whiting, Gary J.

1994-01-01

Net ecosystem CO2 exchange was measured during the 1990 growing season (June to August) along a transect starting 10 km inland from James Bay and extending 100 km interior to Kinosheo Lake, Ontario. Sites were chosen in three distinct areas: a coastal fen, an interior fen, and a bog. For the most productive sites in the bog, net daily uptake rates reached a maximum of 2.5 g C-CO2 m(exp -2)/d with an area-weighted exchange of 0.3 g C-CO2 m(exp -2)/d near midsummer. This site was estimated to be a net carbon source of 9 g C-CO2 m(exp -2) to the atmosphere over a 153-day growing season. The interior fen was less productive on a daily basis with a net maximum uptake of 0.5 g C-CO2 m(exp -2)/d and with corresponding area-weighted uptake of 0.1 g C-CO2 m(exp -2)/d during midsummer. Early and late season release of carbon to the atmosphere resulted in a net loss of 21 g C-CO2 m(exp -2) over the growing season from this site. The coastal fen was the most productive site with uptake rates peaking near 1.7 g C-CO2 m(exp -2)/d which corresponded to an area-weighted uptake of 0.8 g C-CO2 m(exp -2)/d during midsummer and an estimated net uptake of 6 g C-CO2 m(exp -2) for the growing season. Associated with net CO2 exchange measurements, multispectral reflectance properties of the sites were measured over the growing season using portable radiometers. These properties were related to exchange rates with the goal of examining the potential for satellite remote sensing to monitor biosphere/atmosphere CO2 exchange in this biome. The normalized difference vegetation index (NDVI) computed from surface reflectance was correlated with net CO2 exchange for all sites with the exception of areas with large proportions of Sphagnum moss cover. These mosses have greater near-infrared reflectance than typical surrounding vegetation and may require special adjustment for regional exchange/remote sensing applications.

The Detection of Circumnuclear X-Ray Emission from the Seyfert Galaxy NGC 3516

NASA Technical Reports Server (NTRS)

George, I. M.; Turner, T. J.; Netzer, H.; Kraemer, S. B.; Ruiz, J.; Chelouche, D.; Crenshaw, D. M.; Yaqoob, T.; Nandra, K.; Mushotzky, R. F.;

Microorganisms, Organic Carbon, and Their Relationship with Oxidant Activity in Hyper-Arid Mars-Like Soils: Implications for Soil Habitability

NASA Technical Reports Server (NTRS)

Valdivia-Silva, Julio E.; Karouia, Fathi; Navarro-Gonzalez, Rafael; McKay, Christopher

2016-01-01

Soil samples from the hyper-arid region in the Atacama 23 Desert in Southern Peru (La Joya Desert) were analyzed for total and labile organic carbon (TOC & LOC), phospholipid fatty acids analysis (PLFA), quantitative real time polymerase chain reaction (qRT-PCR), 4',6- diamidino-2-phenylindole (DAPI)-fluorescent microscopy, culturable microorganisms, and oxidant activity, in order to understand the relationship between the presence of organic matter and microorganisms in these types of soils. TOC content levels were similar to the labile pool of carbon suggesting the absence of recalcitrant carbon in these soils. The range of LOC was from 2 to 60 micro-g/g of soil. PLFA analysis indicated a maximum of 2.3 x 10(exp 5) cell equivalents/g. Culturing of soil extracts yielded 1.1 x 10(exp 2)-3.7 x 10(exp 3) CFU/g. qRT-PCR showed between 1.0 x 10(exp 2) and 8 x 10(exp 3) cells/g; and DAPI fluorescent staining indicated bacteria counts up to 5 x 104 cells/g. Arid and semiarid samples (controls) showed values between 10(exp 7) and 10(exp 11) cells/g with all of the methods used. Importantly, the concentration of microorganisms in hyper-arid soils did not show any correlation with the organic carbon content; however, there was a significant dependence on the oxidant activity present in these soil samples evaluated as the capacity to decompose sodium formate in 10 hours. We suggest that the analysis of oxidant activity could be a useful indicator of the microbial habitability in hyper-arid soils, obviating the need to measure water activity over time. This approach could be useful in astrobiological studies on other worlds.

A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora.

PubMed Central

Pirhonen, M; Flego, D; Heikinheimo, R; Palva, E T

1993-01-01

Virulence of the plant pathogen Erwinia carotovora subsp. carotovora is dependent on the production and secretion of a complex arsenal of plant cell wall-degrading enzymes. Production of these exoenzymes is controlled by a global regulatory mechanism. A virulent mutants in one of the regulatory loci, expI, show a pleiotropic defect in the growth phase-dependent transcriptional activation of exoenzyme gene expression. The expI gene encodes a 26 kDa polypeptide that is structurally and functionally related to the luxI gene product of Vibrio fischeri. Functional similarity of expI and luxI has been demonstrated by reciprocal genetic complementation experiments. LuxI controls bioluminescence in V.fischeri in a growth phase-dependent manner by directing the synthesis of the diffusible autoinducer, N-(3-oxohexanoyl) homoserine lactone. E.c. subsp. carotovora expI+ strains or Escherichia coli harboring the cloned expI gene excrete a small diffusible signal molecule that complements the expI mutation of Erwinia as well as a luxI mutation of V.fischeri. This extracellular complementation can also be achieved by E.coli harboring the luxI gene from V.fischeri or by adding the synthetic V.fischeri autoinducer. Both the production of the plant tissue-macerating exoenzymes and the ability of the bacteria to propagate in planta are restored in expI mutants by autoinducer addition. These data suggest that the same signal molecule is employed in control of such diverse processes as virulence in a plant pathogen and bioluminescence in a marine bacterium, and may represent a general mechanism by which bacteria modulate gene expression in response to changing environmental conditions. Images PMID:8508772

A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora.

PubMed

Pirhonen, M; Flego, D; Heikinheimo, R; Palva, E T

1993-06-01

Virulence of the plant pathogen Erwinia carotovora subsp. carotovora is dependent on the production and secretion of a complex arsenal of plant cell wall-degrading enzymes. Production of these exoenzymes is controlled by a global regulatory mechanism. A virulent mutants in one of the regulatory loci, expI, show a pleiotropic defect in the growth phase-dependent transcriptional activation of exoenzyme gene expression. The expI gene encodes a 26 kDa polypeptide that is structurally and functionally related to the luxI gene product of Vibrio fischeri. Functional similarity of expI and luxI has been demonstrated by reciprocal genetic complementation experiments. LuxI controls bioluminescence in V.fischeri in a growth phase-dependent manner by directing the synthesis of the diffusible autoinducer, N-(3-oxohexanoyl) homoserine lactone. E.c. subsp. carotovora expI+ strains or Escherichia coli harboring the cloned expI gene excrete a small diffusible signal molecule that complements the expI mutation of Erwinia as well as a luxI mutation of V.fischeri. This extracellular complementation can also be achieved by E.coli harboring the luxI gene from V.fischeri or by adding the synthetic V.fischeri autoinducer. Both the production of the plant tissue-macerating exoenzymes and the ability of the bacteria to propagate in planta are restored in expI mutants by autoinducer addition. These data suggest that the same signal molecule is employed in control of such diverse processes as virulence in a plant pathogen and bioluminescence in a marine bacterium, and may represent a general mechanism by which bacteria modulate gene expression in response to changing environmental conditions.

Intramuscular pressure: A better tool than EMG to optimize exercise for long-duration space flight

NASA Technical Reports Server (NTRS)

Hargens, A. R.; Ballard, R. E.; Aratow, M.; Crenshaw, A.; Styf, J.; Kahan, N.; Watenpaugh, D. E.

1992-01-01

A serious problem experienced by astronauts during long-duration space flight is muscle atrophy. In order to develop countermeasures for this problem, a simple method for monitoring in vivo function of specific muscles is needed. Previous studies document that both intramuscular pressure (IMP) and electromyography (EMG) provide quantitative indices of muscle contraction force during isometric exercise. However, at present there are no data available concerning the usefulness of IMP versus EMG during dynamic exercise. Methods: IMP (Myopress catheter) and surface EMG activity were measured continuously and simultaneously in the tibalis anterior (TA) and soleus (SOL) muscles of 9 normal male volunteers (28-54 years). These parameters were recorded during both concentric and eccentric exercises which consisted of plantarflexon and dorsiflexon of the ankle joint. A Lido Active Isokinetic Dynamometer concurrently recorded ankle joint torque and position. Results: Intramuscular pressure correlated linearly with contraction force for both SOL (r exp 2 = 0.037) and TA (R exp 2 = 0.716 and r exp 2 = 0.802, respectively). During eccentric exercises, SOL and TA IMP also correlated linearly with contraction force (r(exp 2) = 0.883 and r(exp 2) = 0.904 respectively), but SOL and TA EMG correlated poorly with force (r(exp 2) = 0.489 and r(exp 2) = 0.702 respectively). Conclusion: IMP measurement provides a better index of muscle contraction force than EMG during concentric and eccentric exercise. IMP reflects intrinsic mechanical properties of individual muscles, such as length tension relationships. Although invasive, IMP provides a more powerful tool and EMG for developing exercise hardware and protocols for astronauts exposed to long-duration space flight.

Measurements of Aerodynamic Heat Transfer and Boundary-Layer Transition on a 15 deg. Cone in Free Flight at Supersonic Mach Numbers up to 5.2

NASA Technical Reports Server (NTRS)

Rumsey, Charles B.; Lee, Dorothy B.

1961-01-01

Measurements of aerodynamic heat transfer have been made at several stations on the 15 deg total-angle conical nose of a rocket-propelled model in free flight at Mach numbers up to 5.2. Data are presented for a range of local Mach number just outside the boundary layer from 1.40 to 4.65 and a range of local Reynolds number from 3.8 x 10(exp 6) to 46.5 x 10(exp 6), based on length from the nose tip to a measurement station. Laminar, transitional, and turbulent heat-transfer coefficients were measured. The laminar data were in agreement with laminar theory for cones, and the turbulent data agreed well with turbulent theory for cones using Reynolds number based on length from the nose tip. At a nearly constant ratio of wall to local static temperature of 1.2 the Reynolds number of transition increased from 14 x 10(exp 6) to 30 x 10(exp 6) as Mach number increased from 1.4 to 2.9 and then decreased to 17 x 10(exp 6) as Mach number increased to 3.7. At Mach numbers near 3.5, transition Reynolds numbers appeared to be independent of skin temperature at skin temperatures very cold with respect to adiabatic wall temperature. The transition Reynolds number was 17.7 x 10(exp 6) at a condition of Mach number and ratio of wall to local static temperature near that for which three-dimensional disturbance theory has been evaluated and has predicted laminar boundary-layer stability to very high Reynolds numbers (approximately 10(exp 12)).

Detection of Expansin Proteins and Activity during Tomato Fruit Ontogeny1

PubMed Central

Rose, Jocelyn K.C.; Cosgrove, Daniel J.; Albersheim, Peter; Darvill, Alan G.; Bennett, Alan B.

2000-01-01

Expansins are plant proteins that have the capacity to induce extension in isolated cell walls and are thought to mediate pH-dependent cell expansion. J.K.C. Rose, H.H. Lee, and A.B. Bennett ([1997] Proc Natl Acad Sci USA 94: 5955–5960) reported the identification of an expansin gene (LeExp1) that is specifically expressed in ripening tomato (Lycopersicon esculentum) fruit where cell wall disassembly, but not cell expansion, is prominent. Expansin expression during fruit ontogeny was examined using antibodies raised to recombinant LeExp1 or a cell elongation-related expansin from cucumber (CsExp1). The LeExp1 antiserum detected expansins in extracts from ripe, but not preripe tomato fruit, in agreement with the pattern of LeExp1 mRNA accumulation. In contrast, antibodies to CsExp1 cross-reacted with expansins in early fruit development and the onset of ripening, but not at a later ripening stage. These data suggest that ripening-related and expansion-related expansin proteins have distinct antigenic epitopes despite overall high sequence identity. Expansin proteins were detected in a range of fruit species and showed considerable variation in abundance; however, appreciable levels of expansin were not present in fruit of the rin or Nr tomato mutants that exhibit delayed and reduced softening. LeExp1 protein accumulation was ethylene-regulated and matched the previously described expression of mRNA, suggesting that expression is not regulated at the level of translation. We report the first detection of expansin activity in several stages of fruit development and while characteristic creep activity was detected in young and developing tomato fruit and in ripe pear, avocado, and pepper, creep activity in ripe tomato showed qualitative differences, suggesting both hydrolytic and expansin activities. PMID:10938374

Peripheral myopization and visual performance with experimental rigid gas permeable and soft contact lens design.

PubMed

Pauné, J; Queiros, A; Quevedo, L; Neves, H; Lopes-Ferreira, D; González-Méijome, J M

2014-12-01

To evaluate the performance of two experimental contact lenses (CL) designed to induce relative peripheral myopic defocus in myopic eyes. Ten right eyes of 10 subjects were fitted with three different CL: a soft experimental lens (ExpSCL), a rigid gas permeable experimental lens (ExpRGP) and a standard RGP lens made of the same material (StdRGP). Central and peripheral refraction was measured using a Grand Seiko open-field autorefractometer across the central 60° of the horizontal visual field. Ocular aberrations were measured with a Hartman-Shack aberrometer, and monocular contrast sensitivity function (CSF) was measured with a VCTS6500 without and with the three contact lenses. Both experimental lenses were able to increase significantly the relative peripheral myopic defocus up to -0.50 D in the nasal field and -1.00 D in the temporal field (p<0.05). The ExpRGP induced a significantly higher myopic defocus in the temporal field compared to the ExpSCL. ExpSCL induced significantly lower levels of Spherical-like HOA than ExpRGP for the 5mm pupil size (p<0.05). Both experimental lenses kept CSF within normal limits without any statistically significant change from baseline (p>0.05). RGP lens design seems to be more effective to induce a significant myopic change in the relative peripheral refractive error. Both lenses preserve a good visual performance. The worsened optical quality observed in ExpRGP was due to an increased coma-like and spherical-like HOA. However, no impact on the visual quality as measured by CSF was observed. Copyright © 2014 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved.

Comparison of Two Methods for Calculating the Frictional Properties of Articular Cartilage Using a Simple Pendulum and Intact Mouse Knee Joints

PubMed Central

Drewniak, Elizabeth I.; Jay, Gregory D.; Fleming, Braden C.; Crisco, Joseph J.

2009-01-01

In attempts to better understand the etiology of osteoarthritis, a debilitating joint disease that results in the degeneration of articular cartilage in synovial joints, researchers have focused on joint tribology, the study of joint friction, lubrication, and wear. Several different approaches have been used to investigate the frictional properties of articular cartilage. In this study, we examined two analysis methods for calculating the coefficient of friction (μ) using a simple pendulum system and BL6 murine knee joints (n=10) as the fulcrum. A Stanton linear decay model (Lin μ) and an exponential model that accounts for viscous damping (Exp μ) were fit to the decaying pendulum oscillations. Root mean square error (RMSE), asymptotic standard error (ASE), and coefficient of variation (CV) were calculated to evaluate the fit and measurement precision of each model. This investigation demonstrated that while Lin μ was more repeatable, based on CV (5.0% for Lin μ; 18% for Exp μ), Exp μ provided a better fitting model, based on RMSE (0.165° for Exp μ; 0.391° for Lin μ) and ASE (0.033 for Exp μ; 0.185 for Lin μ), and had a significantly lower coefficient of friction value (0.022±0.007 for Exp μ; 0.042±0.016 for Lin μ) (p=0.001). This study details the use of a simple pendulum for examining cartilage properties in situ that will have applications investigating cartilage mechanics in a variety of species. The Exp μ model provided a more accurate fit to the experimental data for predicting the frictional properties of intact joints in pendulum systems. PMID:19632680

BRCA1 and TP53 Gene-Mutations: Family Predisposition and Radioecological Risk of Developing Breast Cancer.

PubMed

Apsalikov, Bakytbek; Manambaeva, Zukhra; Ospanov, Erlan; Massabayeva, Meruyert; Zhabagin, Kuantkan; Zhagiparova, Zhanar; Maximov, Vladymir; Voropaeva, Elena; Apsalikov, Kazbek; Belikhina, Tatiana; Abdrahmanov, Ramil; Cherepkova, Elena; Tanatarov, Sayat; Massadykov, Adilzhan; Urazalina, Naylia

2016-01-01

Frequencies of polymorphisms of genes BRCA1 and TP53 in breast cancer (BC) patients with a BC family history and radiation history were assessed and compared in the Semey region of Kazakhstan. The study included 60 women directly irradiated by the activities of the Semipalatinsk test site with a calculated effective equivalent dose of 500 mSv and their first generation descendants (group BC+Her+Exp); 65 women with family BC and absence of radiological history - the effective equivalent dose due to anthropogenic sources not exceeding 50 mSv (group BC+Her-Exp). The comparison group consisted of 65 women patients with breast cancer without family and radiological history (BC-Her-Exp). The control group comprised 60 women without breast cancer and without family and radiological history (nonBC). We carried out the genotyping of the polymorphisms c.2311T>C, c.4308T>C and 5382insC of the BRCA1 gene and rs1042522 of the TP53 gene. The frequency of the polymorphism c.2311T>C was significantly higher in patients of the group BC+Her+Exp than in healthy women, and of the polymorphism 5382insC in BC+Her+Exp compared to all other groups. The frequency of the rs1042522 polymorphism of TP53 was significantly higher in all groups of patients with breast cancer compared with the control group. Differences between groups of women with breast cancer were significant only in BC+Her+Exp vs. BC+Her-Exp. Combinations of polymorphisms of the genes BRCA1 and TP53 predominated in women with a family and radiological history.

A burst from a thermonuclear runaway on an ONeMg white dwarf

NASA Technical Reports Server (NTRS)

Starrfield, S.; Politano, M.; Truran, J. W.; Sparks, W. M.

1992-01-01

Studies which examine the consequences of accretion, at rates of 10(exp -9) solar mass/yr and 10(exp -10) solar mass/yr, onto an ONeMg white dwarf with a mass of 1.35 solar masses are performed. In these studies, a Lagrangian, hydrodynamic, one-dimensional computer code was used. The code now includes a network with 89 nuclei up to Ca-40, elemental diffusion, new opacities, and new equation of state. The initial abundance distribution corresponded to a mixture that was enriched to either 25, 50, or 75 percent in products of carbon burning. The remaining material in each case is assumed to have a solar composition. The evolution of the thermonuclear runaway in the 1.35 solar mass white dwarf, with M = 10(exp -9) solar mass, produced peak temperatures in the shell source exceeding 300 million degrees. The sequence produced significant amounts of Na-22 from proton captures onto Ne-20 and significant amounts of Al-26 from proton captures on Mg-24. This sequence ejected 5.2 x 10(exp -6) solar mass moving with speeds from approximately 100 km/s to 2300 km/s. When the mass accretion rate was decreased to 10(exp -10) solar mass, the resulting thermonuclear runaway produced a shock that moved through the outer envelope of the white dwarf and raised the surface luminosity to L greater than 10(exp 7) solar luminosity and the effective temperature to values exceeding 10(exp 7) K. The interaction of the material expanding from off of the white dwarf with the accretion disk should produce a burst of gamma-rays.

Experimental hydrophilic vinyl polysiloxane (VPS) impression materials incorporating a novel surfactant compared with commercial VPS.

PubMed

Ud Din, Shahab; Parker, Sandra; Braden, Michael; Tomlins, Pete; Patel, Mangala

2017-08-01

To formulate experimental hydrophilic (Exp) VPS impression materials incorporating a novel surfactant (Rhodasurf CET-2), and to compare their contact angles (CAs) with commercial materials, before/after disinfection. CAs were measured immediately after setting and after disinfection (1% NaOCl; 30min and 24h), together with their change whilst a droplet remained on the materials surface (over 10, 20, 30 60 and 120s), on three commercial (Aquasil Ultra-Monophase [Aq M], Elite HD-Monophase [Elt M], Extrude Medium-bodied [Extr M]) and four experimental (Exp I-IV) materials, using the Drop Shape Analysis 100 technique. The results were compared statistically. CAs of all experimental materials were within the range of those obtained for the commercial materials, with the exception of Exp-IV, which presented with the lowest CAs at the three time points. The control Exp-I was hydrophobic at all three time points (CAs ∼100+), as was Elite. Immediately after setting, Aq M had low CAs but these increased significantly after 30min of disinfection. After twenty four hours' disinfection CAs of all Exp/commercial VPS increased significantly compared to immediately after setting. The CAs of droplets left on the material (120s) decreased with time, even after disinfection, except for Exp-I. The novel surfactant Rhodasurf CET-2 in Exp-III and IV, is an effective surfactant, retaining a low CA after disinfection, compared with Igepal CO-530 in Aq M. Disinfecting VPS impression materials for more than 30min increases their surface CAs, and therefore prolonged disinfection periods should be avoided. Copyright © 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Hypervelocity impact survivability experiments for carbonaceous impactors, part 2

NASA Technical Reports Server (NTRS)

Bunch, T. E.; Paque, Julie M.; Becker, Luann; Vedder, James F.; Erlichman, Jozef

1995-01-01

Hypervelocity impact experiments were performed to further test the survivability of carbonaceous impactors and to determine potential products that may have been synthesized during impact. Diamonds were launched by the Ames two-stage light gas gun into Al plate at velocities of 2.75 and 3.1 km sec(exp -1). FESEM imagery confirms that diamond fragments survived in both experiments. Earlier experiments found that diamonds were destroyed on impact above 4.3 km sec(exp -1). Thus, the upper stability limit for diamond on impact into Al, as determined from our experimental conditions, is between 3.1 and 4.3 km sec(exp -1). Particles of the carbonaceous chondrite Nogoya were also launched into Al at a velocity of 6.2 km sec (exp -1). Laser desorption (L (exp 2) MS) analyses of the impactor residues indicate that the lowest and highest mass polycyclic aromatic hydrocarbons (PAH's) were largely destroyed on impact; those of intermediate mass (202-220 amu) remained at the same level or increased in abundance. In addition, alkyl-substituted homologs of the most abundant pre-impacted PAH's were synthesized during impact. These results suggest that an unknown fraction of some organic compounds can survive low to moderate impact velocities and that synthesized products can be expected to form up to velocities of, at least, 6.5 km sec(exp -1). We also present examples of craters formed by a unique microparticle accelerator that could launch micron-sized particles of almost any coherent material at velocities up to approximately 15 km sec(exp -1). Many of the experiments have a direct bearing on the interpretation of LDEF craters.

Fabrication of sensitive high Tc bolometers

NASA Technical Reports Server (NTRS)

Nahum, Michael; Verghese, S.; Hu, Qing; Richards, Paul L.; Char, K.; Newman, N.; Sachtjen, Scott A.

1990-01-01

The rapid change of resistance with temperature of high quality films of high T sub c superconductors can be used to make resistance thermometers with very low temperature noise. Measurements on c-axis yttrium barium copper oxide (YBCO) films have given a spectral intensity of temperature noise less than 4 times 10(exp -8) K/Hz(exp 1/2) at 10 Hz. Consequently, the opportunity exists to make useful bolometric infrared detectors that operate near 90 K which can be cooled with liquid nitrogen. The fabrication and measurement of two bolometer architectures are discussed. The first is a conventional bolometer which consists of a 3000 A thick YBCO film deposited in situ by laser ablation on top of a 500 A thick SrTiO3 thickness and diced into 1x1 mm(exp 2) bolometer chips. Gold black smoke was used as the radiation absorber. The voltage noise was less than the amplifier noise when the film was current biased. Optical measurements gave an NEP of 5 times 10(exp -11) W/Hz(exp 1/2) at 10 Hz. The second architecture is that of an antenna-coupled microbolometer which consists of a small (5x10 cubic microns) YBCO film deposited directly on a bulk substrate with a low thermal conductance (YSZ) and an impedance matched planar lithographed spiral or log-periodic antenna. This structure is produced by standard photolithographic techniques. Measurements gave an electrical NEP of 4.7 times 10(exp -12) W/Hz(exp 1/2) at 10 kHz. Measurements of the optical efficiency are in progress. The measured performance of both bolometers will be compared to other detectors operating at or above liquid nitrogen temperatures so as to identify potential applications.

Use of spray-dried porcine blood by-products in diets for young chickens.

PubMed

Jamroz, D; Wiliczkiewicz, A; Orda, J; Kuryszko, J; Stefaniak, T

2012-04-01

Spray-dried porcine blood plasma (SDBP) or blood cells (SDBC) at amounts of 20 or 40 g/kg were included to the feed mixtures that were given to young chickens within 1-28 (Exp. 1) or 1-30 (Exp. 2) days post-hatch. In comparison with the group fed mixtures containing plant components, chickens fed mixtures supplemented with 40 g/kg of SDBP significantly (p < 0.01) increased the body weight estimated on 14 day of life (Exp. 1). At the age of 28 or 30 days post-hatch, the body weight was improved significantly (p < 0.01 or 0.05) in both experiments. Significant differences (one-factorial anova) in feed conversion among particular feeding groups were stated in Exp. 1 only; however, calculations using two-factorial anova show insignificant differences depending on the used animal meal. In selected blood parameters (IgG, Ht, Hb), insignificant differences between feeding groups were stated. The use of SDBP in feed mixture significantly increased the Na retention in both experiments, and K accretion in Exp. 1 only. Application of SDBC and 40 g/kg of SDBP significantly or insignificantly improved Fe retention. Insignificant diversification of apparent ileal digestibility of nutrients was stated; the crude fat was significantly better digested in treatments fed mixtures with animal meals but kind of animal meal was without any significant effect. Significant differences in digestibility of amino acids were recorded for Pro, Cys, Val, His, Lys and Arg. In chickens fed mixture with SDBC, higher coefficients of apparent digestibility of Cys, Val and His (Exp. 1) and Cys and His (Exp. 2) than in other feeding groups were obtained. The kind of used blood by-products has not affected the histological structure of intestine wall. © 2011 Blackwell Verlag GmbH.

Experimental Air-Broadened Line Parameters in the nu2 Band of CH3D

NASA Technical Reports Server (NTRS)

Cross, Adriana Predoi; Brawley-Tremblay, Shannon; Povey, Chad; Smith, Mary Ann H.

2007-01-01

In this study we report the first experimental measurements of air-broadening and air-induced pressure-shift coefficients for approximately 378 transitions in the nu2 fundamental band of CH3D. These results were obtained from analysis of 17 room temperature laboratory absorption spectra recorded at 0.0056 cm(exp -1) resolution using the McMath-Pierce Fourier transform spectrometer located on Kitt Peak, Arizona. Three absorption cells with path lengths of 10.2, 25 and 150 cm were used to record the spectra. The total sample pressures ranged from 0.129x10(exp -2) to 52.855x10(exp -2) atm with CH3D volume mixing ratios of approximately 0.0109 in air. The spectra were analyzed using a multispectrum non-linear least-squares fitting technique. We report measurements for air pressure-broadening coefficients for transitions with quantum numbers as high as J" = 20 and K = 15, where K" = K' equivalent to K (for a parallel band). The measured air broadening coefficients range from 0.0205 to 0.0835 cm(exp -1) atm(exp -1) at 296 K. All the measured pressure-shift coefficients are negative and are found to vary from about -0.0005 to -0.0080 cm(exp -1) atm(exp -1) at the temperature of the spectra. We have examined the dependence of the measured broadening and shift parameters on the J" and K quantum numbers and also developed empirical expressions to describe the broadening coefficients in terms of m (m = -J", J" and J" + 1 in the (sup Q)P- (sup Q)Q-, and (sup Q)R-branch, respectively) and K. On average, the empirical expressions reproduce the measured broadening coefficients to within 4.4%.

Influence of omega-3 polyunsaturated fatty acids from fish oil or meal on the structure of lipid microdomains in bovine luteal cells.

PubMed

Plewes, M R; Burns, P D; Graham, P E; Bruemmer, J E; Engle, T E

2018-06-01

Biological membranes are composed of a lipid bilayer and proteins that form lipid microdomains. This study examined the effects of fish byproducts on lipid-protein interactions within lipid microdomains of bovine luteal cells. In Exp. 1 and 2, luteal cells were prepared from corpora lutea (CL; n = 4 to 8) collected at an abattoir. Exp. 1 was conducted to optimize ultrasonication in a detergent-free protocol for isolation of lipid microdomains. A power setting of 10 to 20% was effective in isolating lipid microdomains from bulk lipid. In Exp. 2, cells were cultured in control medium or fish oil to determine influence of fish oil on distribution of lipid microdomain markers and prostaglandin F 2α (FP) receptors. Cells treated with fish oil had a smaller percentage of microdomain markers and FP receptor in microdomains (P < 0.05). In Exp. 3 and 4, cells were prepared from mid-cycle CL obtained from cows supplemented with corn gluten meal (n = 4) or fish meal (n = 4). Exp. 3 examined effects of dietary supplementation on distribution of lipid microdomain markers and FP receptor and Exp. 4 on fatty acid composition within lipid microdomains. A smaller percentage of lipid microdomain markers and FP receptor was detected in microdomains of cells collected from fish meal supplemented animals (P < 0.05). In Exp. 4, a greater percentage of omega-3 polyunsaturated fatty acids was detected in bulk lipid from fish meal supplemented cows (P < 0.05). Results show that fish byproducts influence lipid-protein interactions in lipid microdomains in bovine luteal cells. Copyright © 2018 Elsevier B.V. All rights reserved.

The Transport of Density Fluctuations Throughout the Heliosphere

NASA Technical Reports Server (NTRS)

Zank, G. P.; Jetha, N.; Hu, Q.; Hunana, P.

2012-01-01

The solar wind is recognized as a turbulent magnetofluid, for which the properties of the turbulent velocity and magnetic field fluctuations are often described by the equations of incompressible magnetohydrodynamics (MHD). However, low-frequency density turbulence is also ubiquitous. On the basis of a nearly incompressible formulation of MHD in the expanding inhomogeneous solar wind, we derive the transport equation for the variance of the density fluctuations (Rho(exp 2)). The transport equation shows that density fluctuations behave as a passive scalar in the supersonic solar wind. In the absence of sources of density turbulence, such as within 1AU, the variance (Rho(exp 2)) approximates r(exp -4). In the outer heliosphere beyond 1 AU, the shear between fast and slow streams, the propagation of shocks, and the creation of interstellar pickup ions all act as sources of density turbulence. The model density fluctuation variance evolves with heliocentric distance within approximately 300 AU as (Rho(exp 2)) approximates r(exp -3.3) after which it flattens and then slowly increases. This is precisely the radial profile for the density fluctuation variance observed by Voyager 2. Using a different analysis technique, we confirm the radial profile for Rho(exp 2) of Bellamy, Cairns, & Smith using Voyager 2 data. We conclude that a passive scalar description for density fluctuations in the supersonic solar wind can explain the density fluctuation variance observed in both the inner and the outer heliosphere.

Monensin and ammoniation or urea supplementation of bermudagrass hay diets for steers.

PubMed

Vagnoni, D B; Craig, W M; Gates, R N; Wyatt, W E; Southern, L L

1995-06-01

One growth trial using crossbred beef steers (297 +/- 2 kg, Exp. 1) and one metabolism trial using Holstein steers (405 +/- 14 kg, Exp. 2) were conducted to evaluate effects of ammoniation (3% of hay DM, added as anhydrous NH3) vs urea supplementation with or without monensin supplementation of mature 'Alicia' bermudagrass hay-based diets for steers. Ammoniation (P < .05), but not urea (P = .26) or monensin (P = .70) supplementation, increased ADG in Exp. 1. In Exp. 2 ammoniation increased hay DMI (P < .05), but urea (P = .88) or monensin (P = .16) had no effect. Supplementation with either monensin (P < .05) or urea (P < .05) decreased ruminal total VFA concentrations. Monensin also decreased the ruminal acetate:propionate ratio (P < .001) and increased ruminal pH (P < .05). Addition of urea to the concentrate supplement decreased the rate of concentrate intake. Both the rate (P < .05) and the potential extent (P < .001) of ruminal forage in situ DM and NDF disappearance were increased by ammonia treatment. Dietary interactions (P < .10) were detected between monensin and ammoniation for rate and potential extent of in situ NDF disappearance and between monensin and urea supplementation (P < .05) for ruminal total free amino acid concentrations. Decreased concentrations of VFA and lower forage digestibilities observed with monensin supplementation in Exp. 2 may explain the failure of monensin to affect ADG in Exp. 1.

Neutral Solar Wind Generated by Lunar Exospheric Dust at the Terminator

NASA Technical Reports Server (NTRS)

Collier, Michael R.; Stubbs, Timothy J.

2007-01-01

We calculate the flux of neutral solar wind observed on the lunar surface at the terminator due to solar wind protons penetrating exospheric dust with: (1) grains larger that 0.1 microns and (2) grains larger than 0.01 microns. For grains larger than 0.1 microns, the ratio of the neutral solar wind to solar wind flux is estimated to be approx.10(exp -4)-10(exp -3) at solar wind speeds in excess of 800 km/s, but much lower (less than 10(exp -5) at average to low solar wind speeds. However, when the smaller grain sizes are considered, the ratio of the neutral solar wind flux to solar wind flux is estimated to be greater than or equal to 10(exp -5) at all speeds and at speeds in excess of 700 km/s reaches 10(exp -3)-10(exp -2). These neutral solar wind fluxes are easily measurable with current low energy neutral atom instrumentation. Observations of neutral solar wind from the surface of the Moon could provide a very sensitive determination of the distribution of very small dust grains in the lunar exosphere and would provide data complementary to optical measurements at ultraviolet and visible wavelengths. Furthermore, neutral solar wind, unlike its ionized counterpart, is .not held-off by magnetic anomalies, and may contribute to greater space weathering than expected in certain lunar locations.

Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.

PubMed

Minoia, Silvia; Boualem, Adnane; Marcel, Fabien; Troadec, Christelle; Quemener, Bernard; Cellini, Francesco; Petrozza, Angelo; Vigouroux, Jacqueline; Lahaye, Marc; Carriero, Filomena; Bendahmane, Abdelhafid

2016-01-01

Fruit ripening and softening are key traits for many fleshy fruit. Since cell walls play a key role in the softening process, expansins have been investigated to control fruit over ripening and deterioration. In tomato, expression of Expansin 1 gene, SlExp1, during fruit ripening was associated with fruit softening. To engineer tomato plants with long shelf life, we screened for mutant plants impaired in SlExp1 function. Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening. Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents. Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant. Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure. These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Radon 222 tracing of soil and forest canopy trace gas exchange in an open canopy boreal forest

NASA Technical Reports Server (NTRS)

Ussler, William, III; Chanton, Jeffrey P.; Kelley, Cheryl A.; Martens, Christopher S.

1994-01-01

A set of continuous, high-resolution atmospheric radon (Rn-222) concentration time series and radon soil flux measurements were acquired during the summer of 1990 at a micrometeorological tower site 13 km northwest of Schefferville, Quebec, Canada. The tower was located in a dry upland, open-canopy lichen-spruce woodland. For the period July 23 to August 1, 1990, the mean radon soil flux was 41.1 +/- 4.8 Bq m(exp -2)/h. Radon surface flux from the two end-member forest floor cover types (lichen mat and bare soil) were 38.8 +/- 5.1 and 61.8 +/- 15.6 Bq m(exp -2)/h, respectively. Average total forest canopy resistances computed using a simple 'flux box' model for radon exchange between the forest canopy and the overlying atmosphere range from 0.47 +/- 0.24 s cm(exp -1) to 2.65 +/- 1.61 cm(exp -1) for daytime hours (0900-1700 LT) and from 3.44 +/- 0.91 s cm(exp -1) to 10.55 +/- 7.16 s cm(exp -1) for nighttime hours (2000-0600) for the period July 23 to August 6, 1990. Continuous radon profiling of canopy atmospheres is a suitable approach for determining rates of biosphere/atmosphere trace gas exchange for remote field sites where daily equipment maintenance is not possible. where daily equipment maintenance is not possible.

[Fibromyalgia and working ability--experience of a rheumatological appreciation center].

PubMed

Van Linthoudt, D

2004-07-28

Fibromyalgia is a painful syndrome frequently leading to a rheumatological expertise. Between January 1998 and August 2003, 260 medical appraisals were carried out in the department. Forty-three people matched the fibromyalgia classification criteria proposed by the American College of Rheumatology. This number represents a twofold increase compared to the period ranging from 1968 to 1997. One quarter were men. The group of people with fibromyalgia (Exp. FM+) was compared with a group of patients undergoing a similar evaluation without fibromyalgia (Exp. FM-), matched for age and sex. There were no differences concerning the demographic characteristics nor increase in obesity, heart rate or decreased blood pressure in the Exp. FM+ group. In people mentioning back pain, Waddell tests for a non organic origin of the pain tended to be more present in this group. There were no more triggering events, heavy labor workers or a decreased education level in the Exp. FM+ group. Fatigue, sadness, irritability and evocation of suicide were more frequently noted in the fibromyalgia group. People with work disablement higher than 50% were no more numerous in the Exp. FM+ group. Similarly, the working ability level in the last job or in an appropriated job was not decreased in the Exp. FM+ group. In this study, comparison between people with and without fibromyalgia yielded no great differences and working ability was regarded to be equivalent.

Methionine+cystine requirement of broiler chickens fed low-density diets under tropical conditions.

PubMed

Aftab, Usama; Ashraf, Muhammad

2009-03-01

Two experiments were conducted to determine the M+C requirement of straight-run broiler chickens (Hubbard x Hubbard) during the period 4-21 (Exp. 1) and 21-40 (Exp. 2) days of age. Experiments were conducted during summer months (July-August) in open-sided houses, thus exposing chicks to chronic heat stress. Daily min-max temperature averaged 26-37C (Exp. 1) and 23-36C (Exp. 2). M+C deficient basal diets were formulated to contain low-nutrient-density, i.e., 2750 kcal per kg ME, 20.1% CP (Exp. 1), and 2780 kcal per kg ME, 17.0% CP (Exp. 2). Diets were supplemented with DL-methionine to provide total M+C level ranging from 0.64 to 0.89 % (six increments) and 0.54 to 0.79% (six increments), respectively in experiment 1 and 2. Requirements (0.95 of the maximum quadratic response) were found to be 0.77 and 0.75% total M+C, respectively for gain and feed efficiency, during 4-21 days; and 0.67% total M+C for both gain and feed efficiency during 21-40 days of age. Calculated on the digestible M+C basis, the estimates were 0.67 and 0.65% respectively for gain and feed efficiency during 4-21 days of age; and 0.60% for gain and feed efficiency during 21-40 days of age.