Sample records for export performance requirements

  1. 31 CFR 560.520 - Exportation of agricultural commodities on contracts entered into prior to May 7, 1995.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... States to Iran of any agricultural commodity under an export sales contract are authorized, provided: (1... require delivery of the commodity prior to February 2, 1996. (b) The performance of letters of credit and... such exportation is pursuant to an export sales contract and the conditions contained in paragraphs (a...

  2. 78 FR 35195 - Acquisition Regulations: Export Control

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-12

    ... Control AGENCY: Department of Energy. ACTION: Notice of proposed rulemaking. SUMMARY: The Department of... control requirements applicable to the performance of DOE contracts. DATES: Written comments on this... comments, identified by ``DEAR: Export Control and RIN 1991-AB99,'' by any of the following methods...

  3. 19 CFR 10.430 - Export requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Export requirements. 10.430 Section 10.430 Customs... Export Requirements § 10.430 Export requirements. (a) Submission of certification to CBP. An exporter or producer in the United States that signs a certification of origin for a good exported from the United...

  4. 40 CFR 168.85 - Other export requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Other export requirements. 168.85... STATEMENTS OF ENFORCEMENT POLICIES AND INTERPRETATIONS Export Policy and Procedures for Exporting Unregistered Pesticides § 168.85 Other export requirements. This section describes other requirements found in...

  5. 7 CFR 51.2840 - Export packing requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Export packing requirements. 51.2840 Section 51.2840 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards...) Export Packing Requirements § 51.2840 Export packing requirements. Onions specified as meeting Export...

  6. 50 CFR 222.205 - Import and export requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Import and export requirements. 222.205... Certificates of Exemption for Pre-Act Endangered Species Parts § 222.205 Import and export requirements. (a... intended for importation into or exportation from the United States, shall not be imported or exported...

  7. 9 CFR 91.3 - General export requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false General export requirements. 91.3... HANDLING OF LIVESTOCK FOR EXPORTATION General Provisions § 91.3 General export requirements. (a) All... accompanied from the State of origin of the export movement to the port of embarkation by an origin health...

  8. 77 FR 13990 - Export Sales Reporting Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-08

    ... DEPARTMENT OF AGRICULTURE Office of the Secretary 7 CFR Part 20 RIN 0551-AA70 Export Sales... grain (DDG) to the Export Sales Reporting Requirements. Under this proposed rule, all exporters of U.S. pork and DDG would be required to report on a weekly basis, information on the export sales of pork and...

  9. 21 CFR 312.110 - Import and export requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 5 2010-04-01 2010-04-01 false Import and export requirements. 312.110 Section... export requirements. (a) Imports. An investigational new drug offered for import into the United States.... (b) Exports. An investigational new drug may be exported from the United States for use in a clinical...

  10. An aminoacylation-dependent nuclear tRNA export pathway in yeast.

    PubMed

    Grosshans, H; Hurt, E; Simos, G

    2000-04-01

    Yeast Los1p, the homolog of human exportin-t, mediates nuclear export of tRNA. Using fluorescence in situ hybridization, we could show that the export of some intronless tRNA species is not detectably affected by the disruption of LOS1. To find other factors that facilitate tRNA export, we performed a suppressor screen of a synthetically lethal los1 mutant and identified the essential translation elongation factor eEF-1A. Mutations in eEF-1A impaired nuclear export of all tRNAs tested, which included both spliced and intronless species. An even stronger defect in nuclear exit of tRNA was observed under conditions that inhibited tRNA aminoacylation. In all cases, inhibition of tRNA export led to nucleolar accumulation of mature tRNAs. Our data show that tRNA aminoacylation and eEF-1A are required for efficient nuclear tRNA export in yeast and suggest coordination between the protein translation and the nuclear tRNA processing and transport machineries.

  11. An aminoacylation-dependent nuclear tRNA export pathway in yeast

    PubMed Central

    Grosshans, Helge; Hurt, Ed; Simos, George

    2000-01-01

    Yeast Los1p, the homolog of human exportin-t, mediates nuclear export of tRNA. Using fluorescence in situ hybridization, we could show that the export of some intronless tRNA species is not detectably affected by the disruption of LOS1. To find other factors that facilitate tRNA export, we performed a suppressor screen of a synthetically lethal los1 mutant and identified the essential translation elongation factor eEF-1A. Mutations in eEF-1A impaired nuclear export of all tRNAs tested, which included both spliced and intronless species. An even stronger defect in nuclear exit of tRNA was observed under conditions that inhibited tRNA aminoacylation. In all cases, inhibition of tRNA export led to nucleolar accumulation of mature tRNAs. Our data show that tRNA aminoacylation and eEF-1A are required for efficient nuclear tRNA export in yeast and suggest coordination between the protein translation and the nuclear tRNA processing and transport machineries. PMID:10766739

  12. 7 CFR 1494.401 - Performance security.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Performance security. 1494.401 Section 1494.401... Performance security. (a) Requirement to establish performance security. Prior to the submission of an offer to CCC in response to an Invitation, an eligible exporter must establish performance security, in a...

  13. 7 CFR 1494.401 - Performance security.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Performance security. 1494.401 Section 1494.401... Performance security. (a) Requirement to establish performance security. Prior to the submission of an offer to CCC in response to an Invitation, an eligible exporter must establish performance security, in a...

  14. 77 FR 37823 - Export Sales Reporting Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-25

    ... CFR Part 20 RIN 0551-AA81 Export Sales Reporting Requirements AGENCY: Office of the Secretary, USDA... frozen box/primal cuts) and distillers dried grain (DDG) to the Export Sales Reporting Requirements... basis, information on the export sales of pork and DDG to the Foreign Agricultural Service (FAS). DATES...

  15. 50 CFR 300.183 - Permit holder reporting and recordkeeping requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... biweekly report of entries for consumption, exports and re-exports of fish and fish products regulated... products regulated under this subpart except shark fins were entered for consumption, exported, or re..., that is entered for consumption, exported, or re-exported. (3) A biweekly report is not required for...

  16. 50 CFR 300.183 - Permit holder reporting and recordkeeping requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... biweekly report of entries for consumption, exports and re-exports of fish and fish products regulated... products regulated under this subpart except shark fins were entered for consumption, exported, or re..., that is entered for consumption, exported, or re-exported. (3) A biweekly report is not required for...

  17. 50 CFR 300.183 - Permit holder reporting and recordkeeping requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... biweekly report of entries for consumption, exports and re-exports of fish and fish products regulated... products regulated under this subpart except shark fins were entered for consumption, exported, or re..., that is entered for consumption, exported, or re-exported. (3) A biweekly report is not required for...

  18. 7 CFR 1493.50 - Certification requirements for obtaining payment guarantee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program... requirements of § 1493.30, and the exporter will immediately notify CCC if there is a change of circumstances...

  19. 7 CFR 1493.50 - Certification requirements for obtaining payment guarantee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program... requirements of § 1493.30, and the exporter will immediately notify CCC if there is a change of circumstances...

  20. 7 CFR 1493.50 - Certification requirements for obtaining payment guarantee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program... requirements of § 1493.30, and the exporter will immediately notify CCC if there is a change of circumstances...

  1. 76 FR 29991 - Live Goats and Swine for Export; Removal of Certain Testing Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-24

    ... testing of goats and breeding swine intended for export to countries that do not require such tests. This action will facilitate the exportation of goats and breeding swine by eliminating the need to conduct pre... prior to export. Some countries do not require that goats and breeding swine be tested for tuberculosis...

  2. 15 CFR 30.2 - General requirements for filing Electronic Export Information (EEI).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... definitions, specifications, and requirements of the regulations in this part for all export shipments, except... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false General requirements for filing... Requirements § 30.2 General requirements for filing Electronic Export Information (EEI). (a) Filing...

  3. 7 CFR 1493.80 - Evidence of export.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.80 Evidence of export. (a) Report of export. The exporter is required to provide CCC an evidence of export...

  4. 7 CFR 1493.80 - Evidence of export.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.80 Evidence of export. (a) Report of export. The exporter is required to provide CCC an evidence of export...

  5. 7 CFR 1493.80 - Evidence of export.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.80 Evidence of export. (a) Report of export. The exporter is required to provide CCC an evidence of export...

  6. 78 FR 16819 - Export Sales Reporting Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-19

    ... CFR Part 20 Export Sales Reporting Requirements AGENCY: Office of the Secretary, USDA. ACTION... muscle cuts/whether or not boxed) and distillers dried grain (DDG) be added to the Export Sales Reporting... document provides for an additional comment period regarding mandatory export sales reporting for DDG...

  7. 7 CFR 1493.480 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.480 Certification requirements for the... the exporter breaches or violates these certifications with respect to a SCGP payment guarantee, CCC...

  8. 7 CFR 1493.30 - Information required for program participation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.30 Information required for program participation. Before CCC will accept an application...

  9. 7 CFR 1493.480 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.480 Certification requirements for the... the exporter breaches or violates these certifications with respect to a SCGP payment guarantee, CCC...

  10. 7 CFR 1493.30 - Information required for program participation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.30 Information required for program participation. Before CCC will accept an application...

  11. 7 CFR 1493.30 - Information required for program participation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.30 Information required for program participation. Before CCC will accept an application...

  12. 7 CFR 1493.480 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.480 Certification requirements for the... the exporter breaches or violates these certifications with respect to a SCGP payment guarantee, CCC...

  13. 50 CFR 300.185 - Documentation, reporting and recordkeeping requirements for consignment documents and re-export...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... recordkeeping requirements for consignment documents and re-export certificates. 300.185 Section 300.185..., reporting and recordkeeping requirements for consignment documents and re-export certificates. (a) Imports... apply only to entries for consumption. The reporting requirements of paragraph (a)(3) of this section do...

  14. 50 CFR 300.185 - Documentation, reporting and recordkeeping requirements for consignment documents and re-export...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... recordkeeping requirements for consignment documents and re-export certificates. 300.185 Section 300.185..., reporting and recordkeeping requirements for consignment documents and re-export certificates. (a) Imports... apply only to entries for consumption. The reporting requirements of paragraph (a)(3) of this section do...

  15. 50 CFR 300.185 - Documentation, reporting and recordkeeping requirements for consignment documents and re-export...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... recordkeeping requirements for consignment documents and re-export certificates. 300.185 Section 300.185..., reporting and recordkeeping requirements for consignment documents and re-export certificates. (a) Imports... apply only to entries for consumption. The reporting requirements of paragraph (a)(3) of this section do...

  16. Risk assessment and cost-effectiveness of animal health certification methods for livestock export in Somalia

    PubMed Central

    Knight-Jones, T.J.D.; Njeumi, F.; Elsawalhy, A.; Wabacha, J.; Rushton, J.

    2014-01-01

    Livestock export is vital to the Somali economy. To protect Somali livestock exports from costly import bans used to control the international spread of disease, better certification of livestock health status is required. We performed quantitative risk assessment and cost-effectiveness analysis on different health certification protocols for Somali livestock exports for six transboundary diseases. Examining stock at regional markets alone without port inspection and quarantine was inexpensive but was ineffective for all but contagious bovine pleuropneumonia, contagious caprine pleuropneumonia and peste des petits ruminants. While extended pre-export quarantine improves detection of infections that cause clinical disease, if biosecurity is suboptimal quarantine provides an opportunity for transmission and increased risk. Clinical examination, laboratory screening and vaccination of animals for key diseases before entry to the quarantine station reduced the risk of an exported animal being infected. If vaccination could be reliably performed weeks before arrival at quarantine its effect would be greatly enhanced. The optimal certification method depends on the disease. Laboratory diagnostic testing was particularly important for detecting infections with limited clinical signs in male animals (only males are exported); for Rift Valley fever (RVF) the probability of detection was 99% or 0% with and without testing. Based on our findings animal inspection and certification at regional markets combined with quarantine inspection and certification would reduce the risk of exporting infected animals and enhance disease control at the regional level. This is especially so for key priority diseases, that is RVF, foot-and-mouth disease and Brucellosis. Increased data collection and testing should be applied at point of production and export. PMID:24462194

  17. Risk assessment and cost-effectiveness of animal health certification methods for livestock export in Somalia.

    PubMed

    Knight-Jones, T J D; Njeumi, F; Elsawalhy, A; Wabacha, J; Rushton, J

    2014-03-01

    Livestock export is vital to the Somali economy. To protect Somali livestock exports from costly import bans used to control the international spread of disease, better certification of livestock health status is required. We performed quantitative risk assessment and cost-effectiveness analysis on different health certification protocols for Somali livestock exports for six transboundary diseases. Examining stock at regional markets alone without port inspection and quarantine was inexpensive but was ineffective for all but contagious bovine pleuropneumonia, contagious caprine pleuropneumonia and peste des petits ruminants. While extended pre-export quarantine improves detection of infections that cause clinical disease, if biosecurity is suboptimal quarantine provides an opportunity for transmission and increased risk. Clinical examination, laboratory screening and vaccination of animals for key diseases before entry to the quarantine station reduced the risk of an exported animal being infected. If vaccination could be reliably performed weeks before arrival at quarantine its effect would be greatly enhanced. The optimal certification method depends on the disease. Laboratory diagnostic testing was particularly important for detecting infections with limited clinical signs in male animals (only males are exported); for Rift Valley fever (RVF) the probability of detection was 99% or 0% with and without testing. Based on our findings animal inspection and certification at regional markets combined with quarantine inspection and certification would reduce the risk of exporting infected animals and enhance disease control at the regional level. This is especially so for key priority diseases, that is RVF, foot-and-mouth disease and Brucellosis. Increased data collection and testing should be applied at point of production and export. Copyright © 2014 Elsevier B.V. All rights reserved.

  18. 15 CFR 754.5 - Horses for export by sea.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Horses for export by sea. 754.5... CONTROLS § 754.5 Horses for export by sea. (a) License requirement. As indicated by the letters “SS” in the... No. 1 to part 774 of the EAR) a license is required for the export of horses exported by sea to all...

  19. 15 CFR 754.5 - Horses for export by sea.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Horses for export by sea. 754.5... CONTROLS § 754.5 Horses for export by sea. (a) License requirement. As indicated by the letters “SS” in the... No. 1 to part 774 of the EAR) a license is required for the export of horses exported by sea to all...

  20. 15 CFR 754.5 - Horses for export by sea.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Horses for export by sea. 754.5... CONTROLS § 754.5 Horses for export by sea. (a) License requirement. As indicated by the letters “SS” in the... No. 1 to part 774 of the EAR) a license is required for the export of horses exported by sea to all...

  1. 15 CFR 754.5 - Horses for export by sea.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Horses for export by sea. 754.5... CONTROLS § 754.5 Horses for export by sea. (a) License requirement. As indicated by the letters “SS” in the... No. 1 to part 774 of the EAR) a license is required for the export of horses exported by sea to all...

  2. 15 CFR 754.5 - Horses for export by sea.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Horses for export by sea. 754.5... CONTROLS § 754.5 Horses for export by sea. (a) License requirement. As indicated by the letters “SS” in the... No. 1 to part 774 of the EAR) a license is required for the export of horses exported by sea to all...

  3. Reengineering ribosome export.

    PubMed

    Lo, Kai-Yin; Johnson, Arlen W

    2009-03-01

    Large cargoes require multiple receptors for efficient transport through the nuclear pore complex. The 60S ribosomal subunit is one of the bulkiest transport cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2, and Arx1, collaborate in its export. However, only Crm1, recruited by the adapter Nmd3, appears to be conserved for 60S export in higher eukaryotes. We asked if export of the large subunit requires specific receptors. We made protein fusions between mutant Nmd3 and various export receptors. Surprisingly, fusions of Mex67, the tRNA exportin Los1, Mtr2, Cse1, or Msn5 to Nmd3, lacking its Crm1-dependent nuclear export signal (NES), all functioned in export. Furthermore, these chimeric proteins supported 60S export even in the presence of the Crm1 inhibitor leptomycin B, indicating that export was now independent of Crm1. These results suggest that there is not a requirement for a specific export receptor for the large subunit, as recruitment of any receptor will suffice. Finally we show that the addition of an NES directly to the 60S ribosomal subunit protein Rpl3 promotes export. These results imply remarkable flexibility in the export pathway for the 60S subunit and help explain how different export receptors could have evolved in different eukaryotic lineages.

  4. Reengineering Ribosome Export

    PubMed Central

    Lo, Kai-Yin

    2009-01-01

    Large cargoes require multiple receptors for efficient transport through the nuclear pore complex. The 60S ribosomal subunit is one of the bulkiest transport cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2, and Arx1, collaborate in its export. However, only Crm1, recruited by the adapter Nmd3, appears to be conserved for 60S export in higher eukaryotes. We asked if export of the large subunit requires specific receptors. We made protein fusions between mutant Nmd3 and various export receptors. Surprisingly, fusions of Mex67, the tRNA exportin Los1, Mtr2, Cse1, or Msn5 to Nmd3, lacking its Crm1-dependent nuclear export signal (NES), all functioned in export. Furthermore, these chimeric proteins supported 60S export even in the presence of the Crm1 inhibitor leptomycin B, indicating that export was now independent of Crm1. These results suggest that there is not a requirement for a specific export receptor for the large subunit, as recruitment of any receptor will suffice. Finally we show that the addition of an NES directly to the 60S ribosomal subunit protein Rpl3 promotes export. These results imply remarkable flexibility in the export pathway for the 60S subunit and help explain how different export receptors could have evolved in different eukaryotic lineages. PMID:19144820

  5. 22 CFR 123.1 - Requirement for export or temporary import licenses.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... licenses. 123.1 Section 123.1 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS LICENSES FOR THE EXPORT AND TEMPORARY IMPORT OF DEFENSE ARTICLES § 123.1 Requirement for export or temporary import licenses. (a) Any person who intends to export or to import temporarily a defense article...

  6. 7 CFR 1493.470 - Evidence of export.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC... exporter is required to provide CCC an evidence of export report for each shipment made under the payment... participation in any of the following CCC or USDA export program: Export Enhancement Program, Dairy Export...

  7. 7 CFR 1493.80 - Evidence of export.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.80 Evidence of export. (a) Report of export. The exporter is required to provide CCC an...

  8. 7 CFR 800.17 - Special inspection and weighing requirements for sacked export grain.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... sacked export grain. 800.17 Section 800.17 Agriculture Regulations of the Department of Agriculture (Continued) GRAIN INSPECTION, PACKERS AND STOCKYARD ADMINISTRATION (FEDERAL GRAIN INSPECTION SERVICE... Requirements § 800.17 Special inspection and weighing requirements for sacked export grain. (a) General...

  9. Ethical Issues in Transnational Eye Banking.

    PubMed

    Martin, Dominique E; Kelly, Richard; Jones, Gary L A; Machin, Heather; Pollock, Graeme A

    2017-02-01

    To review ethical issues that may arise in the setting of transnational eye banking activities, such as when exporting or importing corneal tissue for transplantation. A principle-based normative analysis of potential common dilemmas in transnational eye banking activities was performed. Transnational activities in eye banking, like those in other fields involving procurement and use of medical products of human origin, may present a number of ethical issues for policy makers and professionals. Key ethical concerns include the potential impact of export or import activities on self-sufficiency of corneal tissue supply within exporting and importing countries; potential disclosure requirements when obtaining consent or authorization for ocular tissue donation when donations may be exported; and difficulties inherent in assuring equity in the allocation of tissues available for export and in establishing and respecting standards of safety and quality across different jurisdictions. Further analysis of specific ethical issues in eye banking is necessary to inform development of guidelines and other governance tools that will assist policy makers and professionals to support ethical practice.

  10. 10 CFR 40.66 - Requirements for advance notice of export shipments of natural uranium.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Requirements for advance notice of export shipments of natural uranium. 40.66 Section 40.66 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE MATERIAL Records, Reports, and Inspections § 40.66 Requirements for advance notice of export shipments of...

  11. 36 CFR 223.87 - Requirements of bidders concerning exports.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Requirements of bidders concerning exports. 223.87 Section 223.87 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... § 223.87 Requirements of bidders concerning exports. In order to have a bid considered responsive for a...

  12. 9 CFR 91.3 - General export requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    .... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

  13. 9 CFR 91.3 - General export requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    .... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

  14. 9 CFR 91.3 - General export requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

  15. 9 CFR 91.3 - General export requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... (c) Testing. All samples for tests required by §§ 91.5 through 91.13 for exportation of animals under... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false General export requirements. 91.3 Section 91.3 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF...

  16. 15 CFR 744.7 - Restrictions on certain exports to and for the use of certain foreign vessels or aircraft.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Export System (AES) record is filed in accordance with the requirements of the Foreign Trade Statistics Regulations (15 CFR part 30), except that an SED or AES record is not required when any of the commodities... as cargo for which a Shipper's Export Declaration (SED) or Automated Export System (AES) record is...

  17. 15 CFR 744.7 - Restrictions on certain exports to and for the use of certain foreign vessels or aircraft.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Export System (AES) record is filed in accordance with the requirements of the Foreign Trade Statistics Regulations (15 CFR part 30), except that an SED or AES record is not required when any of the commodities... as cargo for which a Shipper's Export Declaration (SED) or Automated Export System (AES) record is...

  18. 15 CFR 744.7 - Restrictions on certain exports to and for the use of certain foreign vessels or aircraft.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Export System (AES) record is filed in accordance with the requirements of the Foreign Trade Statistics Regulations (15 CFR part 30), except that an SED or AES record is not required when any of the commodities... as cargo for which a Shipper's Export Declaration (SED) or Automated Export System (AES) record is...

  19. 15 CFR 744.7 - Restrictions on certain exports to and for the use of certain foreign vessels or aircraft.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Export System (AES) record is filed in accordance with the requirements of the Foreign Trade Statistics Regulations (15 CFR part 30), except that an SED or AES record is not required when any of the commodities... as cargo for which a Shipper's Export Declaration (SED) or Automated Export System (AES) record is...

  20. 15 CFR 713.3 - Annual declaration and reporting requirements for exports and imports of Schedule 2 chemicals.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS ACTIVITIES INVOLVING SCHEDULE 2 CHEMICALS § 713.3 Annual declaration and reporting requirements for exports and imports of Schedule 2 chemicals. (a) Declarations and... requirements for exports and imports of Schedule 2 chemicals. 713.3 Section 713.3 Commerce and Foreign Trade...

  1. 22 CFR 122.1 - Registration requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... in the business of either manufacturing or exporting defense articles or furnishing defense services... subchapter, engaging in the business of manufacturing or exporting defense articles or furnishing defense services requires only one occasion of manufacturing or exporting a defense article or furnishing a defense...

  2. 7 CFR 1493.30 - Information required for program participation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.30 Information required for program participation. Before CCC...

  3. 21 CFR 1313.23 - Distribution of export declaration.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Distribution of export declaration. 1313.23... EXPORTATION OF LIST I AND LIST II CHEMICALS Exportation of Listed Chemicals § 1313.23 Distribution of export declaration. The required three copies of the listed chemical export declaration (DEA Form 486) will be...

  4. 7 CFR 1488.9 - Evidence of export.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Evidence of export. 1488.9 Section 1488.9 Agriculture... Export Sales of Agricultural Commodities From Private Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required for Financing § 1488.9 Evidence of export. (a) If the commodity is exported by rail or...

  5. TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulum.

    PubMed

    Santos, António J M; Nogueira, Cristina; Ortega-Bellido, Maria; Malhotra, Vivek

    2016-05-09

    Procollagens, pre-chylomicrons, and pre-very low-density lipoproteins (pre-VLDLs) are too big to fit into conventional COPII-coated vesicles, so how are these bulky cargoes exported from the endoplasmic reticulum (ER)? We have shown that TANGO1 located at the ER exit site is necessary for procollagen export. We report a role for TANGO1 and TANGO1-like (TALI), a chimeric protein resulting from fusion of MIA2 and cTAGE5 gene products, in the export of pre-chylomicrons and pre-VLDLs from the ER. TANGO1 binds TALI, and both interact with apolipoprotein B (ApoB) and are necessary for the recruitment of ApoB-containing lipid particles to ER exit sites for their subsequent export. Although export of ApoB requires the function of both TANGO1 and TALI, the export of procollagen XII by the same cells requires only TANGO1. These findings reveal a general role for TANGO1 in the export of bulky cargoes from the ER and identify a specific requirement for TALI in assisting TANGO1 to export bulky lipid particles. © 2016 Santos et al.

  6. 50 CFR 14.63 - Export declaration requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 1 2010-10-01 2010-10-01 false Export declaration requirements. 14.63 Section 14.63 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR TAKING, POSSESSION, TRANSPORTATION, SALE, PURCHASE, BARTER, EXPORTATION, AND IMPORTATION OF WILDLIFE AND...

  7. 50 CFR 300.189 - Prohibitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... under § 300.182. (b) Import as an entry for consumption, purchase, receive for export, export, or re... are imported, entered for consumption, exported, or re-exported. (g) Fail to comply with the documentation requirements as specified in § 300.186, for the importation, entry for consumption, exportation...

  8. 50 CFR 300.189 - Prohibitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... under § 300.182. (b) Import as an entry for consumption, purchase, receive for export, export, or re... are imported, entered for consumption, exported, or re-exported. (g) Fail to comply with the documentation requirements as specified in § 300.186, for the importation, entry for consumption, exportation...

  9. 50 CFR 300.189 - Prohibitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... under § 300.182. (b) Import as an entry for consumption, purchase, receive for export, export, or re... are imported, entered for consumption, exported, or re-exported. (g) Fail to comply with the documentation requirements as specified in § 300.186, for the importation, entry for consumption, exportation...

  10. 50 CFR 14.64 - Exceptions to export declaration requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 1 2010-10-01 2010-10-01 false Exceptions to export declaration requirements. 14.64 Section 14.64 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF... OF WILDLIFE AND PLANTS IMPORTATION, EXPORTATION, AND TRANSPORTATION OF WILDLIFE Wildlife Declarations...

  11. 50 CFR 23.53 - What are the requirements for obtaining a retrospective CITES document?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... certain specimens for personal use as specified in paragraph (d)(7) of this section. (2) The exporter or re-exporter must notify the Management Authority in the exporting or re-exporting country of the...) In general, except when the exporter or re-exporter and importer have demonstrated they were not...

  12. 40 CFR 799.19 - Chemical imports and exports.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 32 2011-07-01 2011-07-01 false Chemical imports and exports. 799.19... CONTROL ACT (CONTINUED) IDENTIFICATION OF SPECIFIC CHEMICAL SUBSTANCE AND MIXTURE TESTING REQUIREMENTS General Provisions § 799.19 Chemical imports and exports. Persons who export or who intend to export...

  13. 40 CFR 799.19 - Chemical imports and exports.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 33 2012-07-01 2012-07-01 false Chemical imports and exports. 799.19... CONTROL ACT (CONTINUED) IDENTIFICATION OF SPECIFIC CHEMICAL SUBSTANCE AND MIXTURE TESTING REQUIREMENTS General Provisions § 799.19 Chemical imports and exports. Persons who export or who intend to export...

  14. 40 CFR 799.19 - Chemical imports and exports.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 33 2013-07-01 2013-07-01 false Chemical imports and exports. 799.19... CONTROL ACT (CONTINUED) IDENTIFICATION OF SPECIFIC CHEMICAL SUBSTANCE AND MIXTURE TESTING REQUIREMENTS General Provisions § 799.19 Chemical imports and exports. Persons who export or who intend to export...

  15. 40 CFR 799.19 - Chemical imports and exports.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 32 2014-07-01 2014-07-01 false Chemical imports and exports. 799.19... CONTROL ACT (CONTINUED) IDENTIFICATION OF SPECIFIC CHEMICAL SUBSTANCE AND MIXTURE TESTING REQUIREMENTS General Provisions § 799.19 Chemical imports and exports. Persons who export or who intend to export...

  16. A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18

    PubMed Central

    Chi, Binkai; Wang, Ke; Du, Yanhua; Gui, Bin; Chang, Xingya; Wang, Lantian; Fan, Jing; Chen, She; Wu, Xudong; Li, Guohui; Cheng, Hong

    2014-01-01

    Viral RNA elements that facilitate mRNA export are useful tools for identifying cellular RNA export factors. Here we show that hepatitis B virus post-transcriptional element (PRE) is one such element, and using PRE several new cellular mRNA export factors were identified. We found that PRE drastically enhances the cytoplasmic accumulation of cDNA transcripts independent of any viral protein. Systematic deletion analysis revealed the existence of a 116 nt functional Sub-Element of PRE (SEP1). The RNP that forms on the SEP1 RNA was affinity purified, in which TREX components as well as several other proteins were identified. TREX components and the SEP1-associating protein ZC3H18 are required for SEP1-mediated mRNA export. Significantly, ZC3H18 directly binds to the SEP1 RNA, interacts with TREX and is required for stable association of TREX with the SEP1-containing mRNA. Requirements for SEP1-mediated mRNA export are similar to those for splicing-dependent mRNA export. Consistent with these similarities, several SEP1-interacting proteins, including ZC3H18, ARS2, Acinus and Brr2, are required for efficient nuclear export of polyA RNAs. Together, our data indicate that SEP1 enhances mRNA export by recruiting TREX via ZC3H18. The new mRNA export factors that we identified might be involved in cap- and splicing-dependent TREX recruitment to cellular mRNAs. PMID:24782531

  17. 7 CFR 1493.50 - Certification requirements for obtaining payment guarantee.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export... notify CCC if there is a change of circumstances which would cause it to fail to meet such requirements...

  18. 7 CFR 1493.50 - Certification requirements for obtaining payment guarantee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export... notify CCC if there is a change of circumstances which would cause it to fail to meet such requirements...

  19. 77 FR 16768 - Export Sales Reporting Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-22

    ... DEPARTMENT OF AGRICULTURE Office of the Secretary 7 CFR Part 20 RIN 0551-AA70 Export Sales... for pork (fresh, chilled, and frozen box/primal cuts) and distillers dried grain (DDG) to the Export...: Contact Peter W. Burr, Branch Chief, Export Sales Reporting Branch, Import Policies and Export Reporting...

  20. 7 CFR 1493.90 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103... certifications with respect to a GSM-102 or GSM-103 payment guarantee, CCC will have the right, notwithstanding...

  1. 7 CFR 1493.90 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103... certifications with respect to a GSM-102 or GSM-103 payment guarantee, CCC will have the right, notwithstanding...

  2. 7 CFR 1493.90 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103... certifications with respect to a GSM-102 or GSM-103 payment guarantee, CCC will have the right, notwithstanding...

  3. 50 CFR 14.92 - What are the exemptions to the import/export license requirement?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS IMPORTATION, EXPORTATION, AND TRANSPORTATION OF WILDLIFE... subchapter and that are for export only; and (3) Live aquatic invertebrates of the Class Pelecypoda, commonly...

  4. 75 FR 52453 - Entry Requirements for Certain Softwood Lumber Products Exported From Any Country Into the United...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ...)(3)(iii)(B)(3), which states that ``the exporter has paid, or committed to pay, all export charges..., to his best knowledge and belief, that the exporter has paid or committed to pay ``all export charges... discrepancies between the export permit date and the entry summary data. The commenter suggests using the...

  5. 21 CFR 1010.20 - Electronic products intended for export.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Electronic products intended for export. 1010.20... (CONTINUED) RADIOLOGICAL HEALTH PERFORMANCE STANDARDS FOR ELECTRONIC PRODUCTS: GENERAL Exportation of Electronic Products § 1010.20 Electronic products intended for export. The performance standards prescribed...

  6. 21 CFR 1010.20 - Electronic products intended for export.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Electronic products intended for export. 1010.20... (CONTINUED) RADIOLOGICAL HEALTH PERFORMANCE STANDARDS FOR ELECTRONIC PRODUCTS: GENERAL Exportation of Electronic Products § 1010.20 Electronic products intended for export. The performance standards prescribed...

  7. 21 CFR 1010.20 - Electronic products intended for export.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Electronic products intended for export. 1010.20... (CONTINUED) RADIOLOGICAL HEALTH PERFORMANCE STANDARDS FOR ELECTRONIC PRODUCTS: GENERAL Exportation of Electronic Products § 1010.20 Electronic products intended for export. The performance standards prescribed...

  8. Late Maturation Steps Preceding Selective Nuclear Export and Egress of Progeny Parvovirus

    PubMed Central

    Wolfisberg, Raphael; Kempf, Christoph

    2016-01-01

    ABSTRACT Although the mechanism is not well understood, growing evidence indicates that the nonenveloped parvovirus minute virus of mice (MVM) may actively egress before passive release through cell lysis. We have dissected the late maturation steps of the intranuclear progeny with the aims of confirming the existence of active prelytic egress and identifying critical capsid rearrangements required to initiate the process. By performing anion-exchange chromatography (AEX), we separated intranuclear progeny particles by their net surface charges. Apart from empty capsids (EC), two distinct populations of full capsids (FC) arose in the nuclei of infected cells. The earliest population of FC to appear was infectious but, like EC, could not be actively exported from the nucleus. Further maturation of this early population, involving the phosphorylation of surface residues, gave rise to a second, late population with nuclear export potential. While capsid surface phosphorylation was strictly associated with nuclear export capacity, mutational analysis revealed that the phosphoserine-rich N terminus of VP2 (N-VP2) was dispensable, although it contributed to passive release. The reverse situation was observed for the incoming particles, which were dephosphorylated in the endosomes. Our results confirm the existence of active prelytic egress and reveal a late phosphorylation event occurring in the nucleus as a selective factor for initiating the process. IMPORTANCE In general, the process of egress of enveloped viruses is active and involves host cell membranes. However, the release of nonenveloped viruses seems to rely more on cell lysis. At least for some nonenveloped viruses, an active process before passive release by cell lysis has been reported, although the underlying mechanism remains poorly understood. By using the nonenveloped model parvovirus minute virus of mice, we could confirm the existence of an active process of nuclear export and further characterize the associated capsid maturation steps. Following DNA packaging in the nucleus, capsids required further modifications, involving the phosphorylation of surface residues, to acquire nuclear export potential. Inversely, those surface residues were dephosphorylated on entering capsids. These spatially controlled phosphorylation-dephosphorylation events concurred with the nuclear export-import potential required to complete the infectious cycle. PMID:27009963

  9. Late Maturation Steps Preceding Selective Nuclear Export and Egress of Progeny Parvovirus.

    PubMed

    Wolfisberg, Raphael; Kempf, Christoph; Ros, Carlos

    2016-06-01

    Although the mechanism is not well understood, growing evidence indicates that the nonenveloped parvovirus minute virus of mice (MVM) may actively egress before passive release through cell lysis. We have dissected the late maturation steps of the intranuclear progeny with the aims of confirming the existence of active prelytic egress and identifying critical capsid rearrangements required to initiate the process. By performing anion-exchange chromatography (AEX), we separated intranuclear progeny particles by their net surface charges. Apart from empty capsids (EC), two distinct populations of full capsids (FC) arose in the nuclei of infected cells. The earliest population of FC to appear was infectious but, like EC, could not be actively exported from the nucleus. Further maturation of this early population, involving the phosphorylation of surface residues, gave rise to a second, late population with nuclear export potential. While capsid surface phosphorylation was strictly associated with nuclear export capacity, mutational analysis revealed that the phosphoserine-rich N terminus of VP2 (N-VP2) was dispensable, although it contributed to passive release. The reverse situation was observed for the incoming particles, which were dephosphorylated in the endosomes. Our results confirm the existence of active prelytic egress and reveal a late phosphorylation event occurring in the nucleus as a selective factor for initiating the process. In general, the process of egress of enveloped viruses is active and involves host cell membranes. However, the release of nonenveloped viruses seems to rely more on cell lysis. At least for some nonenveloped viruses, an active process before passive release by cell lysis has been reported, although the underlying mechanism remains poorly understood. By using the nonenveloped model parvovirus minute virus of mice, we could confirm the existence of an active process of nuclear export and further characterize the associated capsid maturation steps. Following DNA packaging in the nucleus, capsids required further modifications, involving the phosphorylation of surface residues, to acquire nuclear export potential. Inversely, those surface residues were dephosphorylated on entering capsids. These spatially controlled phosphorylation-dephosphorylation events concurred with the nuclear export-import potential required to complete the infectious cycle. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  10. A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export

    PubMed Central

    Finn, Erin M.; DeRoo, Elise P.; Clement, George W.; Rao, Sheila; Kruse, Sarah E.; Kokanovich, Kate M.; Belanger, Kenneth D.

    2013-01-01

    The transport of proteins between the cytoplasm and nucleus requires interactions between soluble transport receptors (karyopherins) and phenylalanine-glycine (FG) repeat domains on nuclear pore complex proteins (nucleoporins). However, the role of specific FG repeat-containing nucleoporins in nuclear protein export has not been carefully investigated. We have developed a novel kinetic assay to investigate the relative export kinetics mediated by the karyopherin Msn5/Kap142 in yeast containing specific FG-Nup mutations. Using the Msn5 substrate Crz1 as a marker for Msn5-mediated protein export, we observe that deletions of NUP100 or NUP2 result in decreased rates of Crz1 export, while nup60Δ and nup42Δ mutants do not vary significantly from wild type. The decreased Msn5 export rate in nup100Δ was confirmed using Mig1-GFP as a transport substrate. A nup100ΔGLFG mutant shows defects in nuclear export kinetics similar to a nup100Δ deletion. Removal of FG-repeats from Nsp1 also decreases export kinetics, while a loss of Nup1 FXFGs does not. To confirm that our export data reflected functional differences in protein localization, we performed Crz1 transcription activation assays using a CDRE::LacZ reporter gene that is upregulated upon increased transcription activation by Crz1 in vivo. We observe that expression from this reporter increases in nup100ΔGLFG and nsp1ΔFGΔFXFG strains that exhibit decreased Crz1 export kinetics but resembles wild-type levels in nup1ΔFXFG strains that do not exhibit export defects. These data provide evidence that the export of Msn5 is likely mediated by a specific subset of FG-Nups and that the GLFG repeat domain of Nup100 is important for Msn5-mediated nuclear protein export. PMID:23295456

  11. Resource Conservation and Recovery Act (RCRA) Requirements for Previously Consented Exports of Hazardous Waste

    EPA Pesticide Factsheets

    Requirements for 1) Exports from Canada, Chile, Mexico, or non-OECD countries 40 CFR Part 262 Subparts A-D, and previous E 2) Exports for Recovery from OECD Countries Listed in 40 CFR Section 262.58(a)(1) 40 CFR Part 262, Subparts A-D, previous E and H.

  12. 27 CFR 28.144 - Export marks.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Export marks. 28.144... § 28.144 Export marks. (a) General Requirement. In addition to the marks and brands required to be... brewer shall mark the word “Export” on each container or case of beer, or the words “Beer concentrate for...

  13. 15 CFR 740.15 - Aircraft and vessels (AVS).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Automated Export System (AES) record is filed in accordance with the requirements of the Foreign Trade Statistics Regulations (15 CFR part 30), except that an SED or AES record is not required when any of the...'s Export Declaration (SED) or Automated Export System (AES) record is filed in accordance with the...

  14. 15 CFR 740.15 - Aircraft and vessels (AVS).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Automated Export System (AES) record is filed in accordance with the requirements of the Foreign Trade Statistics Regulations (15 CFR part 30), except that an SED or AES record is not required when any of the...'s Export Declaration (SED) or Automated Export System (AES) record is filed in accordance with the...

  15. 15 CFR 740.15 - Aircraft and vessels (AVS).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Automated Export System (AES) record is filed in accordance with the requirements of the Foreign Trade Statistics Regulations (15 CFR part 30), except that an SED or AES record is not required when any of the...'s Export Declaration (SED) or Automated Export System (AES) record is filed in accordance with the...

  16. 50 CFR 23.18 - What CITES documents are required to export Appendix-I wildlife?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false What CITES documents are required to export Appendix-I wildlife? 23.18 Section 23.18 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE..., EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN...

  17. 50 CFR 23.18 - What CITES documents are required to export Appendix-I wildlife?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 9 2013-10-01 2013-10-01 false What CITES documents are required to export Appendix-I wildlife? 23.18 Section 23.18 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE..., EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN...

  18. 50 CFR 23.18 - What CITES documents are required to export Appendix-I wildlife?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 8 2011-10-01 2011-10-01 false What CITES documents are required to export Appendix-I wildlife? 23.18 Section 23.18 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE..., EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN...

  19. 50 CFR 23.18 - What CITES documents are required to export Appendix-I wildlife?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 9 2012-10-01 2012-10-01 false What CITES documents are required to export Appendix-I wildlife? 23.18 Section 23.18 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE..., EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN...

  20. 50 CFR 23.18 - What CITES documents are required to export Appendix-I wildlife?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 9 2014-10-01 2014-10-01 false What CITES documents are required to export Appendix-I wildlife? 23.18 Section 23.18 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE..., EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN...

  1. 40 CFR 80.1430 - Requirements for exporters of renewable fuels.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... calendar year i, in gallons. k = A discrete volume of exported renewable fuel. VOLk = The standardized volume of discrete volume k of exported renewable fuel that the exporter knows or has reason to know is... associated with discrete volume k. Σ = Sum involving all volumes of cellulosic biofuel exported. DCB,i-1...

  2. FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.

    PubMed

    Minamino, Tohru; Kinoshita, Miki; Inoue, Yumi; Morimoto, Yusuke V; Ihara, Kunio; Koya, Satomi; Hara, Noritaka; Nishioka, Noriko; Kojima, Seiji; Homma, Michio; Namba, Keiichi

    2016-06-01

    For construction of the bacterial flagellum, flagellar proteins are exported via its specific export apparatus from the cytoplasm to the distal end of the growing flagellar structure. The flagellar export apparatus consists of a transmembrane (TM) export gate complex and a cytoplasmic ATPase complex consisting of FliH, FliI, and FliJ. FlhA is a TM export gate protein and plays important roles in energy coupling of protein translocation. However, the energy coupling mechanism remains unknown. Here, we performed a cross-complementation assay to measure robustness of the energy transduction system of the export apparatus against genetic perturbations. Vibrio FlhA restored motility of a Salmonella ΔflhA mutant but not that of a ΔfliH-fliI flhB(P28T) ΔflhA mutant. The flgM mutations significantly increased flagellar gene expression levels, allowing Vibrio FlhA to exert its export activity in the ΔfliH-fliI flhB(P28T) ΔflhA mutant. Pull-down assays revealed that the binding affinities of Vibrio FlhA for FliJ and the FlgN-FlgK chaperone-substrate complex were much lower than those of Salmonella FlhA. These suggest that Vibrio FlhA requires the support of FliH and FliI to efficiently and properly interact with FliJ and the FlgN-FlgK complex. We propose that FliH and FliI ensure robust and efficient energy coupling of protein export during flagellar assembly. © 2016 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

  3. DoD STINFO Manager Training Course. Training Manual

    DTIC Science & Technology

    1993-02-01

    The Export Control Classification Number ( ECCN ) 2. Types of controls, e.g., COCOM 3. Requirements, such as: a. Country groups for which a validated...see Export Administration Act EAR - see Export Administration Regulations ECCN - Export Control Classification Number ELINT - Electronic

  4. Template for Notification of Intent to Export Cathode Ray Tubes (CRTs) for Reuse

    EPA Pesticide Factsheets

    Exporters of CRTs for reuse are required to submit a notification of intent to export to EPA’s Office of Enforcement and Compliance Assurance. This document serves as a template for exporters to use when submitting the notice.

  5. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  6. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  7. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  8. 15 CFR 732.5 - Steps regarding Shipper's Export Declaration or Automated Export System record, Destination...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... than anti-terrorism (AT). The only exception to this requirement would be the return of unwanted... be entered on the invoice and on the bill of lading, air waybill, or other export control document... THE EAR § 732.5 Steps regarding Shipper's Export Declaration or Automated Export System record...

  9. 10 CFR 40.66 - Requirements for advance notice of export shipments of natural uranium.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Requirements for advance notice of export shipments of natural uranium. 40.66 Section 40.66 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE... natural uranium. (a) Each licensee authorized to export natural uranium, other than in the form of ore or...

  10. 10 CFR 40.66 - Requirements for advance notice of export shipments of natural uranium.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Requirements for advance notice of export shipments of natural uranium. 40.66 Section 40.66 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE... natural uranium. (a) Each licensee authorized to export natural uranium, other than in the form of ore or...

  11. 10 CFR 40.66 - Requirements for advance notice of export shipments of natural uranium.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Requirements for advance notice of export shipments of natural uranium. 40.66 Section 40.66 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE... natural uranium. (a) Each licensee authorized to export natural uranium, other than in the form of ore or...

  12. 77 FR 75011 - Revisions to Authorization Validated End-User Provisions: Requirement for Notice of Export...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-19

    ... Bureau of Industry and Security (BIS) amends the Export Administration Regulations (EAR) by adding a...-specific conditions under Authorization VEU no longer require a license for export or reexport or become eligible for shipment under a license exception, as set forth in the EAR, VEUs are no longer bound by the...

  13. 40 CFR 707.60 - Applicability and compliance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... will be required for articles, except PCB articles, unless the Agency so requires in the context of... equal to 50 ppm (by weight or volume). (d) Any person who exports or intends to export PCBs or PCB... intent or exportation under TSCA section 12(b), except as specified in § 707.60(c)(3). PCBs and PCB...

  14. 10 CFR 40.66 - Requirements for advance notice of export shipments of natural uranium.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Requirements for advance notice of export shipments of natural uranium. 40.66 Section 40.66 Energy NUCLEAR REGULATORY COMMISSION DOMESTIC LICENSING OF SOURCE... natural uranium. (a) Each licensee authorized to export natural uranium, other than in the form of ore or...

  15. Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export

    PubMed Central

    Neumann, Bettina; Wu, Haijia; Hackmann, Alexandra; Krebber, Heike

    2016-01-01

    The DEAD-box RNA-helicase Dbp5/Rat8 is known for its function in nuclear mRNA export, where it displaces the export receptor Mex67 from the mRNA at the cytoplasmic side of the nuclear pore complex (NPC). Here we show that Dbp5 is also required for the nuclear export of both pre-ribosomal subunits. Yeast temperature-sensitive dbp5 mutants accumulate both ribosomal particles in their nuclei. Furthermore, Dbp5 genetically and physically interacts with known ribosomal transport factors such as Nmd3. Similar to mRNA export we show that also for ribosomal transport Dbp5 is required at the cytoplasmic side of the NPC. However, unlike its role in mRNA export, Dbp5 does not seem to undergo its ATPase cycle for this function, as ATPase-deficient dbp5 mutants that selectively inhibit mRNA export do not affect ribosomal transport. Furthermore, mutants of GLE1, the ATPase stimulating factor of Dbp5, show no major ribosomal export defects. Consequently, while Dbp5 uses its ATPase cycle to displace the export receptor Mex67 from the translocated mRNAs, Mex67 remains bound to ribosomal subunits upon transit to the cytoplasm, where it is detectable on translating ribosomes. Therefore, we propose a model, in which Dbp5 supports ribosomal transport by capturing ribosomal subunits upon their cytoplasmic appearance at the NPC, possibly by binding export factors such as Mex67. Thus, our findings reveal that although different ribonucleoparticles, mRNAs and pre-ribosomal subunits, use shared export factors, they utilize different transport mechanisms. PMID:26872259

  16. Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export.

    PubMed

    Neumann, Bettina; Wu, Haijia; Hackmann, Alexandra; Krebber, Heike

    2016-01-01

    The DEAD-box RNA-helicase Dbp5/Rat8 is known for its function in nuclear mRNA export, where it displaces the export receptor Mex67 from the mRNA at the cytoplasmic side of the nuclear pore complex (NPC). Here we show that Dbp5 is also required for the nuclear export of both pre-ribosomal subunits. Yeast temperature-sensitive dbp5 mutants accumulate both ribosomal particles in their nuclei. Furthermore, Dbp5 genetically and physically interacts with known ribosomal transport factors such as Nmd3. Similar to mRNA export we show that also for ribosomal transport Dbp5 is required at the cytoplasmic side of the NPC. However, unlike its role in mRNA export, Dbp5 does not seem to undergo its ATPase cycle for this function, as ATPase-deficient dbp5 mutants that selectively inhibit mRNA export do not affect ribosomal transport. Furthermore, mutants of GLE1, the ATPase stimulating factor of Dbp5, show no major ribosomal export defects. Consequently, while Dbp5 uses its ATPase cycle to displace the export receptor Mex67 from the translocated mRNAs, Mex67 remains bound to ribosomal subunits upon transit to the cytoplasm, where it is detectable on translating ribosomes. Therefore, we propose a model, in which Dbp5 supports ribosomal transport by capturing ribosomal subunits upon their cytoplasmic appearance at the NPC, possibly by binding export factors such as Mex67. Thus, our findings reveal that although different ribonucleoparticles, mRNAs and pre-ribosomal subunits, use shared export factors, they utilize different transport mechanisms.

  17. 48 CFR 235.071 - Export-controlled items.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Export-controlled items..., DEPARTMENT OF DEFENSE SPECIAL CATEGORIES OF CONTRACTING RESEARCH AND DEVELOPMENT CONTRACTING 235.071 Export-controlled items. For requirements regarding access to export-controlled items, see Subpart 204.73. [73 FR...

  18. 48 CFR 1852.225-70 - Export Licenses.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... writing, that the Contracting Officer authorizes it to export ITAR-controlled technical data (including... licenses or other approvals, if required, for exports of hardware, technical data, and software, or for the provision of technical assistance. (b) The Contractor shall be responsible for obtaining export licenses, if...

  19. 15 CFR 30.15 - Introduction.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... export control purposes. All parties to an export transaction must comply with all relevant export... convenience, references to provisions of the EAR, ITAR, CBP, and OFAC regulations that affect the statistical... information may be required for export control purposes by the regulations of CBP, BIS, State Department, or...

  20. 15 CFR 30.15 - Introduction.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... export control purposes. All parties to an export transaction must comply with all relevant export... convenience, references to provisions of the EAR, ITAR, CBP, and OFAC regulations that affect the statistical... information may be required for export control purposes by the regulations of CBP, BIS, State Department, or...

  1. 15 CFR 30.15 - Introduction.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... export control purposes. All parties to an export transaction must comply with all relevant export... convenience, references to provisions of the EAR, ITAR, CBP, and OFAC regulations that affect the statistical... information may be required for export control purposes by the regulations of CBP, BIS, State Department, or...

  2. 15 CFR 30.15 - Introduction.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... export control purposes. All parties to an export transaction must comply with all relevant export... convenience, references to provisions of the EAR, ITAR, CBP, and OFAC regulations that affect the statistical... information may be required for export control purposes by the regulations of CBP, BIS, State Department, or...

  3. 7 CFR 1488.8 - Documents required after delivery.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AGRICULTURAL COMMODITIES Financing of Export Sales of Agricultural Commodities From Private Stocks Under CCC... delivery. (a) CCC will purchase an exporter's account receivable only if the Treasurer, Commodity Credit... or Assistant Treasurer, CCC, after date of delivery of commodities exported or to be exported under...

  4. 7 CFR 1493.100 - Proof of entry.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.100 Proof of entry... translation acceptable to CCC. Records acceptable to meet this requirement include an original certification...

  5. 7 CFR 1493.100 - Proof of entry.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.100 Proof of entry... translation acceptable to CCC. Records acceptable to meet this requirement include an original certification...

  6. 7 CFR 1488.8 - Documents required after delivery.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AGRICULTURAL COMMODITIES Financing of Export Sales of Agricultural Commodities From Private Stocks Under CCC... delivery. (a) CCC will purchase an exporter's account receivable only if the Treasurer, Commodity Credit... or Assistant Treasurer, CCC, after date of delivery of commodities exported or to be exported under...

  7. 7 CFR 1493.100 - Proof of entry.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493.100 Proof of entry... translation acceptable to CCC. Records acceptable to meet this requirement include an original certification...

  8. 40 CFR 262.52 - General requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 262.52 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE Exports of Hazardous Waste § 262.52 General requirements. Exports of hazardous waste are prohibited except in compliance with the applicable requirements...

  9. 22 CFR 123.25 - Amendments to licenses.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... approve an amendment to a license for permanent export, temporary export and temporary import of... Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS LICENSES FOR THE EXPORT OF... forwarder and the export does not involve technical data. A new license is required for these changes. Any...

  10. 22 CFR 123.25 - Amendments to licenses.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... approve an amendment to a license for permanent export, temporary export and temporary import of... Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS LICENSES FOR THE EXPORT OF... forwarder and the export does not involve technical data. A new license is required for these changes. Any...

  11. 50 CFR 20.64 - Foreign export permits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Foreign export permits. 20.64 Section 20... WILDLIFE AND PLANTS (CONTINUED) MIGRATORY BIRD HUNTING Importations § 20.64 Foreign export permits. No... such birds are accompanied by export permits, tags, or other documentation required by applicable...

  12. 15 CFR 30.16 - Export Administration Regulations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Export Administration Regulations. 30... OF THE CENSUS, DEPARTMENT OF COMMERCE FOREIGN TRADE REGULATIONS Export Control and Licensing Requirements § 30.16 Export Administration Regulations. The EAR issued by the U.S. Department of Commerce, BIS...

  13. 7 CFR 1493.280 - Evidence of export report.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC... exporter is required to provide CCC an evidence of export report for each shipment of goods or provision of... CCC for coverage under the facility payment guarantee and this subpart; (ii) The specifications and...

  14. 15 CFR 714.2 - Annual reporting requirements for exports and imports in excess of 30 metric tons of Schedule 3...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS ACTIVITIES INVOLVING SCHEDULE 3 CHEMICALS § 714.2 Annual reporting requirements for exports and imports in excess of 30 metric tons of... exports and imports in excess of 30 metric tons of Schedule 3 chemicals. 714.2 Section 714.2 Commerce and...

  15. 15 CFR 714.2 - Annual reporting requirements for exports and imports in excess of 30 metric tons of Schedule 3...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS ACTIVITIES INVOLVING SCHEDULE 3 CHEMICALS § 714.2 Annual reporting requirements for exports and imports in excess of 30 metric tons of... exports and imports in excess of 30 metric tons of Schedule 3 chemicals. 714.2 Section 714.2 Commerce and...

  16. 15 CFR 30.2 - General requirements for filing Electronic Export Information (EEI).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... nonforeign areas including goods previously admitted to customs warehouses or FTZs and moving under a U.S... filed prior to exportation (see § 30.4) unless the USPPI has been approved to submit export data on a... this part. (2) Specific data elements required for EEI filing are contained in § 30.6. (3) The AES...

  17. 7 CFR 800.161 - Official certificate requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... applicable; (12) the results of the service performed; (13) the location of the issuing office; (14) the... Service shall be used for all export grain. (Approved by the Office of Management and Budget under control... STOCKYARD ADMINISTRATION (FEDERAL GRAIN INSPECTION SERVICE), DEPARTMENT OF AGRICULTURE GENERAL REGULATIONS...

  18. 75 FR 47608 - Agency Information Collection Activities: Application for Exportation of Articles under Special Bond

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ... Activities: Application for Exportation of Articles under Special Bond AGENCY: U.S. Customs and Border... information collection requirement concerning the: Application for Exportation of Articles under Special Bond... information collection: Title: Application for Exportation of Articles under Special Bond. OMB Number: 1651...

  19. 40 CFR 211.110-3 - Export exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Export exemptions. 211.110-3 Section... PROGRAMS PRODUCT NOISE LABELING General Provisions § 211.110-3 Export exemptions. (a) A new product intended solely for export, and which has satisfied the requirements of other applicable regulations of...

  20. 40 CFR 80.1430 - Requirements for exporters of renewable fuels.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or... associated with discrete volume k. Σ = Sum involving all volumes of biodiesel or renewable diesel exported.... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or...

  1. 40 CFR 80.1430 - Requirements for exporters of renewable fuels.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or... associated with discrete volume k. Σ = Sum involving all volumes of biodiesel or renewable diesel exported.... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or...

  2. 40 CFR 80.1430 - Requirements for exporters of renewable fuels.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or... associated with discrete volume k. Σ = Sum involving all volumes of biodiesel or renewable diesel exported.... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or...

  3. 40 CFR 80.1430 - Requirements for exporters of renewable fuels.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or... associated with discrete volume k. Σ = Sum involving all volumes of biodiesel or renewable diesel exported.... VOLk = The standardized volume of discrete volume k of exported renewable fuel that is biodiesel or...

  4. 7 CFR 1570.1100 - Drawback certification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... certification. An offer submitted by an exporter to FAS for an export bonus under the SOAP or the COAP must contain, in addition to any other information required by FAS, a certification stating the following... officer of the exporter, if the exporter is a partnership or a corporation, respectively. FAS will reject...

  5. 7 CFR 1570.1100 - Drawback certification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... certification. An offer submitted by an exporter to FAS for an export bonus under the SOAP or the COAP must contain, in addition to any other information required by FAS, a certification stating the following... officer of the exporter, if the exporter is a partnership or a corporation, respectively. FAS will reject...

  6. 7 CFR 1570.1100 - Drawback certification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... certification. An offer submitted by an exporter to FAS for an export bonus under the SOAP or the COAP must contain, in addition to any other information required by FAS, a certification stating the following... officer of the exporter, if the exporter is a partnership or a corporation, respectively. FAS will reject...

  7. 7 CFR 1570.1100 - Drawback certification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... certification. An offer submitted by an exporter to FAS for an export bonus under the SOAP or the COAP must contain, in addition to any other information required by FAS, a certification stating the following... officer of the exporter, if the exporter is a partnership or a corporation, respectively. FAS will reject...

  8. 7 CFR 1570.1100 - Drawback certification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... certification. An offer submitted by an exporter to FAS for an export bonus under the SOAP or the COAP must contain, in addition to any other information required by FAS, a certification stating the following... officer of the exporter, if the exporter is a partnership or a corporation, respectively. FAS will reject...

  9. 15 CFR 30.3 - Electronic Export Information filer requirements, parties to export transactions, and...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .../units of measure. (ix) Value. (x) Export Control Classification Number (ECCN) or sufficient technical information to determine the ECCN. (xi) All licensing information necessary to file the EEI for commodities... export. (xi) Foreign port of unloading. (xii) Shipping weight. (xiii) ECCN. (xiv) License or license...

  10. 15 CFR 30.3 - Electronic Export Information filer requirements, parties to export transactions, and...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .../units of measure. (ix) Value. (x) Export Control Classification Number (ECCN) or sufficient technical information to determine the ECCN. (xi) All licensing information necessary to file the EEI for commodities... export. (xi) Foreign port of unloading. (xii) Shipping weight. (xiii) ECCN. (xiv) License or license...

  11. 15 CFR 30.3 - Electronic Export Information filer requirements, parties to export transactions, and...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    .../units of measure. (ix) Value. (x) Export Control Classification Number (ECCN) or sufficient technical information to determine the ECCN. (xi) All licensing information necessary to file the EEI for commodities... export. (xi) Foreign port of unloading. (xii) Shipping weight. (xiii) ECCN. (xiv) License or license...

  12. 15 CFR 30.3 - Electronic Export Information filer requirements, parties to export transactions, and...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    .../units of measure. (ix) Value. (x) Export Control Classification Number (ECCN) or sufficient technical information to determine the ECCN. (xi) All licensing information necessary to file the EEI for commodities... export. (xi) Foreign port of unloading. (xii) Shipping weight. (xiii) ECCN. (xiv) License or license...

  13. 15 CFR 30.3 - Electronic Export Information filer requirements, parties to export transactions, and...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .../units of measure. (ix) Value. (x) Export Control Classification Number (ECCN) or sufficient technical information to determine the ECCN. (xi) All licensing information necessary to file the EEI for commodities... export. (xi) Foreign port of unloading. (xii) Shipping weight. (xiii) ECCN. (xiv) License or license...

  14. 7 CFR 1493.90 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit... breaches or violates these certifications with respect to a GSM-102 or GSM-103 payment guarantee, CCC will...

  15. 7 CFR 1488.9 - Evidence of export.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Agricultural Commodities From Private Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required... exporter shall furnish to the Treasurer, CCC, one copy of the bill of lading covering the commodity..., the exporter shall furnish to the Treasurer, CCC, one non-negotiable copy or photo copy or other type...

  16. 7 CFR 1493.90 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit... breaches or violates these certifications with respect to a GSM-102 or GSM-103 payment guarantee, CCC will...

  17. 7 CFR 1488.9 - Evidence of export.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Agricultural Commodities From Private Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required... exporter shall furnish to the Treasurer, CCC, one copy of the bill of lading covering the commodity..., the exporter shall furnish to the Treasurer, CCC, one non-negotiable copy or photo copy or other type...

  18. 7 CFR 1488.9 - Evidence of export.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Agricultural Commodities From Private Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required... exporter shall furnish to the Treasurer, CCC, one copy of the bill of lading covering the commodity..., the exporter shall furnish to the Treasurer, CCC, one non-negotiable copy or photo copy or other type...

  19. 7 CFR 35.11 - Minimum requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... applicable to the variety and destination specified: (a) Any such variety for export to destinations in Japan... requirements. (b) Any such variety for export to any foreign destination, other than destinations in Japan...

  20. 15 CFR 30.2 - General requirements for filing Electronic Export Information (EEI).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... any mode of transportation. (Instructions for filing EEI for vessels, aircraft, railway cars, and... of export shipments, regardless of value: (A) Requiring a Department of Commerce, Bureau of Industry...

  1. 15 CFR 30.2 - General requirements for filing Electronic Export Information (EEI).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... any mode of transportation. (Instructions for filing EEI for vessels, aircraft, railway cars, and... of export shipments, regardless of value: (A) Requiring a Department of Commerce, Bureau of Industry...

  2. 27 CFR 28.193 - Export marks.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Export marks. 28.193... Drawback Filing of Notice and Removal § 28.193 Export marks. In addition to the marks and brands required... chapter, the exporter shall mark the word “Export” on the Government side of each case or Government head...

  3. 27 CFR 28.223 - Export marks.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Export marks. 28.223... Export marks. In addition to the marks and brands required to be placed on kegs, barrels, cases, crates... “Export” on each container or case before removal for export, for use on vessels or aircraft, or for...

  4. 19 CFR 192.11 - Description of the AES.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Description of the AES. 192.11 Section 192.11... TREASURY (CONTINUED) EXPORT CONTROL Filing of Export Information Through the Automated Export System (AES) § 192.11 Description of the AES. AES is a voluntary program that allows all exporters required to report...

  5. 15 CFR 758.2 - Automated Export System (AES).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Automated Export System (AES). 758.2... CLEARANCE REQUIREMENTS § 758.2 Automated Export System (AES). The Census Bureau's Foreign Trade Statistics...) electronically using the Automated Export System (AES). In order to use AES, you must apply directly to the...

  6. 15 CFR 758.2 - Automated Export System (AES).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Automated Export System (AES). 758.2... CLEARANCE REQUIREMENTS § 758.2 Automated Export System (AES). The Census Bureau's Foreign Trade Statistics...) electronically using the Automated Export System (AES). In order to use AES, you must apply directly to the...

  7. 19 CFR 192.11 - Description of the AES.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Description of the AES. 192.11 Section 192.11... TREASURY (CONTINUED) EXPORT CONTROL Filing of Export Information Through the Automated Export System (AES) § 192.11 Description of the AES. AES is a voluntary program that allows all exporters required to report...

  8. 19 CFR 192.11 - Description of the AES.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Description of the AES. 192.11 Section 192.11... TREASURY (CONTINUED) EXPORT CONTROL Filing of Export Information Through the Automated Export System (AES) § 192.11 Description of the AES. AES is a voluntary program that allows all exporters required to report...

  9. 19 CFR 192.11 - Description of the AES.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Description of the AES. 192.11 Section 192.11... TREASURY (CONTINUED) EXPORT CONTROL Filing of Export Information Through the Automated Export System (AES) § 192.11 Description of the AES. AES is a voluntary program that allows all exporters required to report...

  10. 15 CFR 758.2 - Automated Export System (AES).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Automated Export System (AES). 758.2... CLEARANCE REQUIREMENTS § 758.2 Automated Export System (AES). The Census Bureau's Foreign Trade Statistics...) electronically using the Automated Export System (AES). In order to use AES, you must apply directly to the...

  11. 7 CFR 319.8-17 - Importation for exportation, and importation for transportation and exportation; storage.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... exportation, vacuum fumigation, or utilization in accordance with the requirements in this subpart, may be... movement to an approved mill or plant for vacuum fumigation or utilization, when there are inspectors... of storage in the north pending exportation, fumigation, or utilization in an approved mill or plant...

  12. 7 CFR 319.8-17 - Importation for exportation, and importation for transportation and exportation; storage.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... exportation, vacuum fumigation, or utilization in accordance with the requirements in this subpart, may be... movement to an approved mill or plant for vacuum fumigation or utilization, when there are inspectors... of storage in the north pending exportation, fumigation, or utilization in an approved mill or plant...

  13. 7 CFR 319.8-17 - Importation for exportation, and importation for transportation and exportation; storage.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... exportation, vacuum fumigation, or utilization in accordance with the requirements in this subpart, may be... movement to an approved mill or plant for vacuum fumigation or utilization, when there are inspectors... of storage in the north pending exportation, fumigation, or utilization in an approved mill or plant...

  14. 7 CFR 319.8-17 - Importation for exportation, and importation for transportation and exportation; storage.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... exportation, vacuum fumigation, or utilization in accordance with the requirements in this subpart, may be... movement to an approved mill or plant for vacuum fumigation or utilization, when there are inspectors... of storage in the north pending exportation, fumigation, or utilization in an approved mill or plant...

  15. 7 CFR 1493.440 - Certification requirements for payment guarantee.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.440 Certification requirements for payment... notify CCC if there is a change of circumstances which would cause it to fail to meet such requirements...

  16. 7 CFR 1493.440 - Certification requirements for payment guarantee.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.440 Certification requirements for payment... notify CCC if there is a change of circumstances which would cause it to fail to meet such requirements...

  17. 7 CFR 1493.440 - Certification requirements for payment guarantee.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.440 Certification requirements for payment... notify CCC if there is a change of circumstances which would cause it to fail to meet such requirements...

  18. 19 CFR 122.78 - Entry or withdrawal for exportation or for transportation and exportation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Documents Required for Clearance and Permission To Depart; Electronic Manifest Requirements for Passengers, Crew Members, and Non...

  19. 50 CFR 23.19 - What CITES documents are required to export Appendix-I plants?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... SERVICE, DEPARTMENT OF THE INTERIOR (CONTINUED) TAKING, POSSESSION, TRANSPORTATION, SALE, PURCHASE, BARTER... CITES documents are required to export Appendix-I plants? Answer the questions in the following decision...

  20. The Relationship of Skilled Aerospace Manufacturing Workforce Performance to Training

    ERIC Educational Resources Information Center

    Malsberry, Suzanne

    2014-01-01

    A major economic driver, the aerospace industry contributes to exports and higher wage jobs, which the United States requires to maintain robust economic health. Despite the investment in vocational educational training programs, insufficient workers have been available to aerospace companies. The purpose of this study was to investigate the…

  1. 7 CFR 1488.8 - Documents required after delivery.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Financing of Export Sales of Agricultural Commodities From Private Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required for Financing § 1488.8 Documents required after delivery. (a) CCC will... regulations within forty-five days, or any extension thereof by the Treasurer or Assistant Treasurer, CCC...

  2. 7 CFR 1488.8 - Documents required after delivery.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Financing of Export Sales of Agricultural Commodities From Private Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required for Financing § 1488.8 Documents required after delivery. (a) CCC will... regulations within forty-five days, or any extension thereof by the Treasurer or Assistant Treasurer, CCC...

  3. 7 CFR 1488.8 - Documents required after delivery.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Financing of Export Sales of Agricultural Commodities From Private Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required for Financing § 1488.8 Documents required after delivery. (a) CCC will... regulations within forty-five days, or any extension thereof by the Treasurer or Assistant Treasurer, CCC...

  4. 19 CFR 19.15 - Withdrawal for exportation of articles manufactured in bond; waste or byproducts for consumption.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... direct exportation, unless an authorization of the manufacturer is endorsed on the face of the withdrawal... be required as in the case of other warehouse withdrawals for exportation. (l) When the fact of... 19 Customs Duties 1 2010-04-01 2010-04-01 false Withdrawal for exportation of articles...

  5. 15 CFR 758.1 - The Shipper's Export Declaration (SED) or Automated Export System (AES) record.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... entered on the loading document (e.g., Cargo Declaration, manifest, bill of lading, (master) air waybill... for all items being exported under the NLR provisions that have a reason for control other than anti-terrorism (AT). The designator “TSPA” may be used, but is not required, when the export consists of...

  6. 15 CFR 758.1 - The Shipper's Export Declaration (SED) or Automated Export System (AES) record.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... entered on the loading document (e.g., Cargo Declaration, manifest, bill of lading, (master) air waybill... for all items being exported under the NLR provisions that have a reason for control other than anti-terrorism (AT). The designator “TSPA” may be used, but is not required, when the export consists of...

  7. 15 CFR 745.1 - Advance notification and annual report of all exports of Schedule 1 chemicals to other States...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SECURITY, DEPARTMENT OF COMMERCE EXPORT ADMINISTRATION REGULATIONS CHEMICAL WEAPONS CONVENTION REQUIREMENTS... Prohibition of Chemical Weapons (OPCW) not less than 30 days in advance of every export of a Schedule 1... of all exports of Schedule 1 chemicals to other States Parties. 745.1 Section 745.1 Commerce and...

  8. Interagency Review of Prior Inspector General Recommendations Related to U.S. Export Controls. Volume I

    DTIC Science & Technology

    2008-09-01

    applications that require referral to the Office of Export Enforcement ( OEE ), and sends them simultaneously to OEE and licensing officers in Export...Enforcement ( OEE ) and the Office of Enforcement Analysis (OEA). We also spoke with staff from OEE ’ s Boston field office regarding closed...OExS staff should refer them to the Office of Export Enforcement ( OEE ). However, in the two cases of noncompliance, both exporters had shipped

  9. 50 CFR 23.47 - What are the requirements for export of an Appendix-I plant artificially propagated for...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... permit or re-export certificate based on Appendix-II criteria. The specimen is still listed in Appendix I... propagating operation that qualifies under paragraph (d) of this section, or to persons who have acquired a... qualifying specimen. (3) The export permit, and any subsequent re-export certificate, must show the specimen...

  10. Technical options for processing additional light tight oil volumes within the United States

    EIA Publications

    2015-01-01

    This report examines technical options for processing additional LTO volumes within the United States. Domestic processing of additional LTO would enable an increase in petroleum product exports from the United States, already the world’s largest net exporter of petroleum products. Unlike crude oil, products are not subject to export limitations or licensing requirements. While this is one possible approach to absorbing higher domestic LTO production in the absence of a relaxation of current limitations on crude exports, domestic LTO would have to be priced at a level required to encourage additional LTO runs at existing refinery units, debottlenecking, or possible additions of processing capacity.

  11. Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation.

    PubMed

    Rondón, Ana G; Jimeno, Sonia; García-Rubio, María; Aguilera, Andrés

    2003-10-03

    THO/TREX is a conserved eukaryotic complex formed by the core THO complex plus proteins involved in mRNA metabolism and export such as Sub2 and Yra1. Mutations in any of the THO/TREX structural genes cause pleiotropic phenotypes such as transcription impairment, increased transcription-associated recombination, and mRNA export defects. To assay the relevance of THO/TREX complex in transcription, we performed in vitro transcription elongation assays in mutant cell extracts using supercoiled DNA templates containing two G-less cassettes. With these assays, we demonstrate that hpr1delta, tho2delta, and mft1delta mutants of the THO complex and sub2 mutants show significant reductions in the efficiency of transcription elongation. The mRNA expression defect of hpr1delta mutants was not due to an increase in mRNA decay, as determined by mRNA half-life measurements and mRNA time course accumulation experiments in the absence of Rrp6p exoribonuclease. This work demonstrates that THO and Sub2 are required for efficient transcription elongation, providing further evidence for the coupling between transcription and mRNA metabolism and export.

  12. 21 CFR 314.410 - Imports and exports of new drugs.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., and is shipped with a copy of the labeling required for, the approved drug product. (3) Insulin or an antibiotic drug may be exported without regard to the requirements in section 802 of the act if the insulin...

  13. 21 CFR 314.410 - Imports and exports of new drugs.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., and is shipped with a copy of the labeling required for, the approved drug product. (3) Insulin or an antibiotic drug may be exported without regard to the requirements in section 802 of the act if the insulin...

  14. 21 CFR 314.410 - Imports and exports of new drugs.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., and is shipped with a copy of the labeling required for, the approved drug product. (3) Insulin or an antibiotic drug may be exported without regard to the requirements in section 802 of the act if the insulin...

  15. 21 CFR 314.410 - Imports and exports of new drugs.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., and is shipped with a copy of the labeling required for, the approved drug product. (3) Insulin or an antibiotic drug may be exported without regard to the requirements in section 802 of the act if the insulin...

  16. 9 CFR 351.17 - Identification required.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.17 Identification required. Certified technical animal fats... certified plants shall be accompanied by MP Form 85 (Declaration to Accompany Technical Animal Fats Between...

  17. 9 CFR 351.17 - Identification required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.17 Identification required. Certified technical animal fats... certified plants shall be accompanied by MP Form 85 (Declaration to Accompany Technical Animal Fats Between...

  18. 9 CFR 351.17 - Identification required.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.17 Identification required. Certified technical animal fats... certified plants shall be accompanied by MP Form 85 (Declaration to Accompany Technical Animal Fats Between...

  19. 9 CFR 351.17 - Identification required.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.17 Identification required. Certified technical animal fats... certified plants shall be accompanied by MP Form 85 (Declaration to Accompany Technical Animal Fats Between...

  20. 9 CFR 351.17 - Identification required.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.17 Identification required. Certified technical animal fats... certified plants shall be accompanied by MP Form 85 (Declaration to Accompany Technical Animal Fats Between...

  1. 14 CFR 1274.942 - Export licenses.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... writing, that the Agreement Officer authorize it to export ITAR-controlled technical data (including... appropriate licenses or other approvals, if required, for exports of hardware, technical data, and software, or for the provision of technical assistance. (b) The Recipient shall be responsible for obtaining...

  2. 7 CFR 1493.100 - Proof of entry.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493... a certified or other translation acceptable to CCC. Records acceptable to meet this requirement...

  3. 7 CFR 1493.100 - Proof of entry.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Export Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103) Operations § 1493... a certified or other translation acceptable to CCC. Records acceptable to meet this requirement...

  4. 7 CFR 1487.5 - What is the process for submitting proposals?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... reimbursement will be sought; and (viii) Information indicating all financial and in-kind support to the... services. Although highly encouraged, financial support from the participant is not required. (3) Export... performance measure for the year prior to the year that the project would begin; and (iii) The viability of...

  5. 15 CFR 745.1 - Advance notification and annual report of all exports of Schedule 1 chemicals to other States...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... plan to export any quantity of a Schedule 1 chemical controlled under the EAR and licensed by the... chemicals subject to the EAR or from the Department of State for the export of Schedule 1 chemicals subject... State Party. This is in addition to the requirement to obtain an export license under the EAR for...

  6. 15 CFR 745.1 - Advance notification and annual report of all exports of Schedule 1 chemicals to other States...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... plan to export any quantity of a Schedule 1 chemical controlled under the EAR and licensed by the... chemicals subject to the EAR or from the Department of State for the export of Schedule 1 chemicals subject... State Party. This is in addition to the requirement to obtain an export license under the EAR for...

  7. 15 CFR 745.1 - Advance notification and annual report of all exports of Schedule 1 chemicals to other States...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... plan to export any quantity of a Schedule 1 chemical controlled under the EAR and licensed by the... chemicals subject to the EAR or from the Department of State for the export of Schedule 1 chemicals subject... State Party. This is in addition to the requirement to obtain an export license under the EAR for...

  8. 7 CFR 800.16 - Certification requirements for export grain.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Certification requirements for export grain. 800.16 Section 800.16 Agriculture Regulations of the Department of Agriculture (Continued) GRAIN INSPECTION, PACKERS AND STOCKYARD ADMINISTRATION (FEDERAL GRAIN INSPECTION SERVICE), DEPARTMENT OF AGRICULTURE GENERAL...

  9. 7 CFR 1530.101 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE THE REFINED SUGAR RE-EXPORT PROGRAM, THE SUGAR CONTAINING PRODUCTS RE-EXPORT PROGRAM, AND THE...-packers. Bond or letter of credit means an insurance agreement pledging surety for the entry of foreign sugar without the required re-export within the program guidelines. Certain polyhydric alcohols means...

  10. 31 CFR 538.507 - Reexports by non-U.S. persons.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) OFFICE OF FOREIGN ASSETS CONTROL, DEPARTMENT OF THE TREASURY SUDANESE SANCTIONS REGULATIONS Licenses... technology subject to export license application requirements under other United States regulations. The... the United States, the exportation of which to Sudan is subject to export or reexport license...

  11. 77 FR 22689 - Revisions to Authorization Validated End-User Provisions: Requirement for Notice of Export...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-17

    ...) by adding a requirement for persons shipping under Authorization Validated End- User (VEU) to send... that when items subject to item-specific conditions under Authorization VEU no longer require a license for export or reexport or become eligible for shipment under a license exception, as set forth in the...

  12. Protein Export by the Mycobacterial SecA2 System Is Determined by the Preprotein Mature Domain

    PubMed Central

    Feltcher, Meghan E.; Gibbons, Henry S.; Ligon, Lauren S.

    2013-01-01

    At the core of the bacterial general secretion (Sec) pathway is the SecA ATPase, which powers translocation of unfolded preproteins containing Sec signal sequences through the SecYEG membrane channel. Mycobacteria have two nonredundant SecA homologs: SecA1 and SecA2. While the essential SecA1 handles “housekeeping” export, the nonessential SecA2 exports a subset of proteins and is required for Mycobacterium tuberculosis virulence. Currently, it is not understood how SecA2 contributes to Sec export in mycobacteria. In this study, we focused on identifying the features of two SecA2 substrates that target them to SecA2 for export, the Ms1704 and Ms1712 lipoproteins of the model organism Mycobacterium smegmatis. We found that the mature domains of Ms1704 and Ms1712, not the N-terminal signal sequences, confer SecA2-dependent export. We also demonstrated that the lipid modification and the extreme N terminus of the mature protein do not impart the requirement for SecA2 in export. We further showed that the Ms1704 mature domain can be efficiently exported by the twin-arginine translocation (Tat) pathway. Because the Tat system exports only folded proteins, this result implies that SecA2 substrates can fold in the cytoplasm and suggests a putative role of SecA2 in enabling export of such proteins. Thus, the mycobacterial SecA2 system may represent another way that bacteria solve the problem of exporting proteins that can fold in the cytoplasm. PMID:23204463

  13. 50 CFR 222.205 - Import and export requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS GENERAL ENDANGERED AND THREATENED MARINE SPECIES Certificates of Exemption for Pre-Act Endangered Species Parts § 222.205 Import and export requirements. (a... neither endangered nor threatened species and that are imported for purposes of human or animal...

  14. 50 CFR 222.205 - Import and export requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS GENERAL ENDANGERED AND THREATENED MARINE SPECIES Certificates of Exemption for Pre-Act Endangered Species Parts § 222.205 Import and export requirements. (a... neither endangered nor threatened species and that are imported for purposes of human or animal...

  15. 50 CFR 222.205 - Import and export requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS GENERAL ENDANGERED AND THREATENED MARINE SPECIES Certificates of Exemption for Pre-Act Endangered Species Parts § 222.205 Import and export requirements. (a... neither endangered nor threatened species and that are imported for purposes of human or animal...

  16. 50 CFR 222.205 - Import and export requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS GENERAL ENDANGERED AND THREATENED MARINE SPECIES Certificates of Exemption for Pre-Act Endangered Species Parts § 222.205 Import and export requirements. (a... neither endangered nor threatened species and that are imported for purposes of human or animal...

  17. 40 CFR 205.5-3 - Export exemptions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ....5-3 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) NOISE ABATEMENT PROGRAMS TRANSPORTATION EQUIPMENT NOISE EMISSION CONTROLS General Provisions § 205.5-3 Export exemptions. (a) A new.... (b) No request for an export exemption is required. (c) For purposes of section 11(d) of the Noise...

  18. 14 CFR 1274.942 - Export licenses.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... appropriate licenses or other approvals, if required, for exports of hardware, technical data, and software... installation], where the foreign person will have access to export-controlled technical data or software. (c... software) pursuant to the exemption at 22 CFR 125.4(b)(3). The Agreement Officer or designated...

  19. 78 FR 22843 - Foreign-Trade Zone 33-Pittsburgh, Pennsylvania, Authorization of Export Production Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-17

    ..., Pennsylvania, Authorization of Export Production Activity, Tsudis Chocolate Company (Chocolate Confectionery Bars), Pittsburgh, Pennsylvania On December 4, 2012, Tsudis Chocolate Company, submitted a notification... restriction requiring that all foreign-status liquid chocolate admitted to FTZ 33 must be re-exported. Dated...

  20. 75 FR 33989 - Export Administration Regulations: Technical Corrections

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... 0694-AE69 Export Administration Regulations: Technical Corrections AGENCY: Bureau of Industry and... section of Export Control Classification Number 2B001 and the other is in the Technical Note on Adjusted... language regarding certain performance criteria of turning machines covered by Export Control...

  1. Modeling traceability information and functionality requirement in export-oriented tilapia chain.

    PubMed

    Zhang, Xiaoshuan; Feng, Jianying; Xu, Mark; Hu, Jinyou

    2011-05-01

    Tilapia has been named as the 'food fish of the 21st century' and has become the most important farmed fish. China is the world leader in tilapia production and export. Identifying information and functional requirements is critical in developing an efficient traceability system because traceability has become a fundamental prerequisite for exporting aquaculture products. This paper examines the export-oriented tilapia chains and information flow in the chains, and identifies the key actors, information requirements and information-capturing points. Unified Modeling Language (UML) technology is adopted to describe the information and functionality requirement for chain traceability. The barriers of traceability system adoption are also identified. The results show that the traceability data consist of four categories that must be recorded by each link in the chain. The functionality requirement is classified into four categories from the fundamental information record to decisive quality control; the top three barriers to the traceability system adoption are: high costs of implementing the system, lack of experienced and professional staff; and low level of government involvement and support. Copyright © 2011 Society of Chemical Industry.

  2. Sensitivity analysis of a sediment dynamics model applied in a Mediterranean river basin: global change and management implications.

    PubMed

    Sánchez-Canales, M; López-Benito, A; Acuña, V; Ziv, G; Hamel, P; Chaplin-Kramer, R; Elorza, F J

    2015-01-01

    Climate change and land-use change are major factors influencing sediment dynamics. Models can be used to better understand sediment production and retention by the landscape, although their interpretation is limited by large uncertainties, including model parameter uncertainties. The uncertainties related to parameter selection may be significant and need to be quantified to improve model interpretation for watershed management. In this study, we performed a sensitivity analysis of the InVEST (Integrated Valuation of Environmental Services and Tradeoffs) sediment retention model in order to determine which model parameters had the greatest influence on model outputs, and therefore require special attention during calibration. The estimation of the sediment loads in this model is based on the Universal Soil Loss Equation (USLE). The sensitivity analysis was performed in the Llobregat basin (NE Iberian Peninsula) for exported and retained sediment, which support two different ecosystem service benefits (avoided reservoir sedimentation and improved water quality). Our analysis identified the model parameters related to the natural environment as the most influential for sediment export and retention. Accordingly, small changes in variables such as the magnitude and frequency of extreme rainfall events could cause major changes in sediment dynamics, demonstrating the sensitivity of these dynamics to climate change in Mediterranean basins. Parameters directly related to human activities and decisions (such as cover management factor, C) were also influential, especially for sediment exported. The importance of these human-related parameters in the sediment export process suggests that mitigation measures have the potential to at least partially ameliorate climate-change driven changes in sediment exportation. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Shift in performance of food safety management systems in supply chains: case of green bean chain in Kenya versus hot pepper chain in Uganda.

    PubMed

    Nanyunja, Jessica; Jacxsens, Liesbeth; Kirezieva, Klementina; Kaaya, Archileo N; Uyttendaele, Mieke; Luning, Pieternel A

    2016-08-01

    This study investigates the level of design and operation of food safety management systems (FSMS) of farmers and export traders in Kenya and Uganda. FSMS diagnostic tools developed for the fresh produce chain were used to assess the levels of context riskiness, FSMS activities and system output in primary production (n = 60) and trade (n = 60). High-risk context characteristics combined with basic FSMS are expected to increase the risk on unsafe produce. In Uganda both farmers and export traders of hot peppers operate in a high- to moderate-risk context but have basic FSMS and low systems output. In Kenya, both farmers and export traders of green beans operate in a low- to moderate-risk context. The farmers have average performing FSMS, whereas export trade companies showed more advanced FSMS and system output scores ranging from satisfactory to good. Large retailers supplying the EU premium market play a crucial role in demanding compliance with strict voluntary food safety standards, which was reflected in the more advanced FSMS and good system output in Kenya, especially traders. In Kenya, a clear shift in more fit-for-purpose FSMS and higher system output was noticed between farms and trade companies. In the case of Uganda, traders commonly supply to the less demanding EU wholesale markets such as ethnic specialty shops. They only have to comply with the legal phytosanitary and pesticide residue requirements for export activities, which apparently resulted in basic FSMS and low system output present with both farmers and traders. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  4. SLY1 and Syntaxin 18 specify a distinct pathway for procollagen VII export from the endoplasmic reticulum

    PubMed Central

    Nogueira, Cristina; Erlmann, Patrik; Villeneuve, Julien; Santos, António JM; Martínez-Alonso, Emma; Martínez-Menárguez, José Ángel; Malhotra, Vivek

    2014-01-01

    TANGO1 binds and exports Procollagen VII from the endoplasmic reticulum (ER). In this study, we report a connection between the cytoplasmic domain of TANGO1 and SLY1, a protein that is required for membrane fusion. Knockdown of SLY1 by siRNA arrested Procollagen VII in the ER without affecting the recruitment of COPII components, general protein secretion, and retrograde transport of the KDEL-containing protein BIP, and ERGIC53. SLY1 is known to interact with the ER-specific SNARE proteins Syntaxin 17 and 18, however only Syntaxin 18 was required for Procollagen VII export. Neither SLY1 nor Syntaxin 18 was required for the export of the equally bulky Procollagen I from the ER. Altogether, these findings reveal the sorting of bulky collagen family members by TANGO1 at the ER and highlight the existence of different export pathways for secretory cargoes one of which is mediated by the specific SNARE complex containing SLY1 and Syntaxin 18. DOI: http://dx.doi.org/10.7554/eLife.02784.001 PMID:24842878

  5. 50 CFR 14.63 - Export declaration requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 1 2013-10-01 2013-10-01 false Export declaration requirements. 14.63 Section 14.63 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... furnished is true and complete to the best of his/her knowledge and belief. ...

  6. 50 CFR 14.63 - Export declaration requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 1 2011-10-01 2011-10-01 false Export declaration requirements. 14.63 Section 14.63 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... furnished is true and complete to the best of his/her knowledge and belief. ...

  7. 50 CFR 14.63 - Export declaration requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 1 2012-10-01 2012-10-01 false Export declaration requirements. 14.63 Section 14.63 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... furnished is true and complete to the best of his/her knowledge and belief. ...

  8. 50 CFR 14.63 - Export declaration requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 1 2014-10-01 2014-10-01 false Export declaration requirements. 14.63 Section 14.63 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR... furnished is true and complete to the best of his/her knowledge and belief. ...

  9. 22 CFR 126.10 - Disclosure of information.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ....10 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL TRAFFIC IN ARMS REGULATIONS GENERAL POLICIES... Trade Controls. (b) Determinations required by law. Section 38(e) of the Arms Export Control Act (22 U.S... of certain persons, in accordance with Section 38 of the Arms Export Control Act. The requirements...

  10. 22 CFR 123.16 - Exemptions of general applicability.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... subchapter); MTCR articles; Significant Military Equipment (SME); and may not be used by persons who are..., including those to and from Canada, require a Shipper's Export Declaration (SED) or notification letter. If... § 123.22 for Shipper's Export Declaration and letter notification requirements. (b) The following...

  11. 78 FR 25415 - Waivers Under the Refined Sugar Re-Export Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-01

    ... license amounts and provide greater flexibility to offset exports and transfers with raw sugar imports...-Export Program, refiners may enter raw sugar unrestricted by the quantitative limit established for the raw sugar tariff-rate quota or the requirements of certificates of quota eligibility provided for in...

  12. 78 FR 43849 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ...: Export Fruit Regulations. OMB Control Number: 0581-0143. Summary of Collection: Fresh apples and grapes... requirements established by regulations issued under the Export Apple Act (7 CFR Part 33) and the Export Grape... States in apples and grapes; to protect the reputation of these American-grown commodities; and to...

  13. 7 CFR 1530.101 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AGRICULTURE THE REFINED SUGAR RE-EXPORT PROGRAM, THE SUGAR CONTAINING PRODUCTS RE-EXPORT PROGRAM, AND THE... sugar without the required re-export within the program guidelines. Certain polyhydric alcohols means... as a substitute for sugar as a sweetener in human food. Co-packer means a person who adds value to a...

  14. 7 CFR 1530.101 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AGRICULTURE THE REFINED SUGAR RE-EXPORT PROGRAM, THE SUGAR CONTAINING PRODUCTS RE-EXPORT PROGRAM, AND THE... sugar without the required re-export within the program guidelines. Certain polyhydric alcohols means... as a substitute for sugar as a sweetener in human food. Co-packer means a person who adds value to a...

  15. 40 CFR 168.65 - Pesticide export label and labeling requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... toxic pesticides. If the pesticide, device or active ingredient is highly toxic to humans, the skull and... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pesticide export label and labeling...) PESTICIDE PROGRAMS STATEMENTS OF ENFORCEMENT POLICIES AND INTERPRETATIONS Export Policy and Procedures for...

  16. 40 CFR 168.65 - Pesticide export label and labeling requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... toxic pesticides. If the pesticide, device or active ingredient is highly toxic to humans, the skull and... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Pesticide export label and labeling...) PESTICIDE PROGRAMS STATEMENTS OF ENFORCEMENT POLICIES AND INTERPRETATIONS Export Policy and Procedures for...

  17. 40 CFR 168.65 - Pesticide export label and labeling requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... toxic pesticides. If the pesticide, device or active ingredient is highly toxic to humans, the skull and... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Pesticide export label and labeling...) PESTICIDE PROGRAMS STATEMENTS OF ENFORCEMENT POLICIES AND INTERPRETATIONS Export Policy and Procedures for...

  18. 48 CFR 1852.225-70 - Export Licenses.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... licenses or other approvals, if required, for exports of hardware, technical data, and software, or for the... will have access to export-controlled technical data or software. (c) The Contractor shall be... software) pursuant to the exemption at 22 CFR 125.4(b)(3). The Contracting Officer or designated...

  19. 15 CFR 30.6 - Electronic Export Information data elements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Electronic Export Information data elements. 30.6 Section 30.6 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade... Electronic Export Information data elements. The information specified in this section is required for...

  20. 15 CFR 30.6 - Electronic Export Information data elements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Electronic Export Information data elements. 30.6 Section 30.6 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade... Electronic Export Information data elements. The information specified in this section is required for...

  1. 7 CFR 1530.101 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE THE REFINED SUGAR RE-EXPORT PROGRAM, THE SUGAR CONTAINING PRODUCTS RE-EXPORT PROGRAM, AND THE... sugar without the required re-export within the program guidelines. Certain polyhydric alcohols means... as a substitute for sugar as a sweetener in human food. Co-packer means a person who adds value to a...

  2. 7 CFR 1530.101 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE THE REFINED SUGAR RE-EXPORT PROGRAM, THE SUGAR CONTAINING PRODUCTS RE-EXPORT PROGRAM, AND THE... sugar without the required re-export within the program guidelines. Certain polyhydric alcohols means... as a substitute for sugar as a sweetener in human food. Co-packer means a person who adds value to a...

  3. 7 CFR 20.9 - Records.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Records. 20.9 Section 20.9 Agriculture Office of the Secretary of Agriculture EXPORT SALES REPORTING REQUIREMENTS § 20.9 Records. Each reporting exporter shall establish and maintain accurate records as to all export sales of commodities subject to these regulations...

  4. 7 CFR 20.9 - Records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Records. 20.9 Section 20.9 Agriculture Office of the Secretary of Agriculture EXPORT SALES REPORTING REQUIREMENTS § 20.9 Records. Each reporting exporter shall establish and maintain accurate records as to all export sales of commodities subject to these regulations...

  5. 78 FR 5802 - Agency Information Collection Activities: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-28

    ... Review and Comments Request. Form Title: EIB 92-31 Notification by Insured of Amounts Payable Under Multi-Buyer Export Credit Insurance policy (Standard Assignment). SUMMARY: The Export-Import Bank of the..., as required by the Paperwork Reduction Act of 1995. This form represents the exporter's directive to...

  6. 77 FR 44258 - Agency Information Collection Activities: Exportation of Used Self-Propelled Vehicles

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-27

    ... Activities: Exportation of Used Self-Propelled Vehicles AGENCY: U.S. Customs and Border Protection (CBP... information collection requirement concerning the Exportation of Used Self-Propelled Vehicles. This request... clarity of the information to be collected; (d) ways to minimize the burden including the use of automated...

  7. Role of ER Export Signals in Controlling Surface Potassium Channel Numbers

    NASA Astrophysics Data System (ADS)

    Ma, Dzwokai; Zerangue, Noa; Lin, Yu-Fung; Collins, Anthony; Yu, Mei; Jan, Yuh Nung; Yeh Jan, Lily

    2001-01-01

    Little is known about the identity of endoplasmic reticulum (ER) export signals and how they are used to regulate the number of proteins on the cell surface. Here, we describe two ER export signals that profoundly altered the steady-state distribution of potassium channels and were required for channel localization to the plasma membrane. When transferred to other potassium channels or a G protein-coupled receptor, these ER export signals increased the number of functional proteins on the cell surface. Thus, ER export of membrane proteins is not necessarily limited by folding or assembly, but may be under the control of specific export signals.

  8. Revision of Import and Export Requirements for Controlled Substances, Listed Chemicals, and Tableting and Encapsulating Machines, Including Changes To Implement the International Trade Data System (ITDS); Revision of Reporting Requirements for Domestic Transactions in Listed Chemicals and Tableting and Encapsulating Machines; and Technical Amendments. Final rule.

    PubMed

    2016-12-30

    The Drug Enforcement Administration is updating its regulations for the import and export of tableting and encapsulating machines, controlled substances, and listed chemicals, and its regulations relating to reports required for domestic transactions in listed chemicals, gamma-hydroxybutyric acid, and tableting and encapsulating machines. In accordance with Executive Order 13563, the Drug Enforcement Administration has reviewed its import and export regulations and reporting requirements for domestic transactions in listed chemicals (and gamma-hydroxybutyric acid) and tableting and encapsulating machines, and evaluated them for clarity, consistency, continued accuracy, and effectiveness. The amendments clarify certain policies and reflect current procedures and technological advancements. The amendments also allow for the implementation, as applicable to tableting and encapsulating machines, controlled substances, and listed chemicals, of the President's Executive Order 13659 on streamlining the export/import process and requiring the government-wide utilization of the International Trade Data System (ITDS). This rule additionally contains amendments that implement recent changes to the Controlled Substances Import and Export Act (CSIEA) for reexportation of controlled substances among members of the European Economic Area made by the Improving Regulatory Transparency for New Medical Therapies Act. The rule also includes additional substantive and technical and stylistic amendments.

  9. Exporters for Production of Amino Acids and Other Small Molecules.

    PubMed

    Eggeling, Lothar

    Microbes are talented catalysts to synthesize valuable small molecules in their cytosol. However, to make full use of their skills - and that of metabolic engineers - the export of intracellularly synthesized molecules to the culture medium has to be considered. This step is as essential as is each step for the synthesis of the favorite molecule of the metabolic engineer, but is frequently not taken into account. To export small molecules via the microbial cell envelope, a range of different types of carrier proteins is recognized to be involved, which are primary active carriers, secondary active carriers, or proteins increasing diffusion. Relevant export may require just one carrier as is the case with L-lysine export by Corynebacterium glutamicum or involve up to four carriers as known for L-cysteine excretion by Escherichia coli. Meanwhile carriers for a number of small molecules of biotechnological interest are recognized, like for production of peptides, nucleosides, diamines, organic acids, or biofuels. In addition to carriers involved in amino acid excretion, such carriers and their impact on product formation are described, as well as the relatedness of export carriers which may serve as a hint to identify further carriers required to improve product formation by engineering export.

  10. A Novel ESX-1 Locus Reveals that Surface-Associated ESX-1 Substrates Mediate Virulence in Mycobacterium marinum

    PubMed Central

    Kennedy, George M.; Hooley, Gwendolyn C.; Champion, Matthew M.; Mba Medie, Felix

    2014-01-01

    EsxA (ESAT-6) and EsxB (CFP-10) are virulence factors exported by the ESX-1 system in mycobacterial pathogens. In Mycobacterium marinum, an established model for ESX-1 secretion in Mycobacterium tuberculosis, genes required for ESX-1 export reside at the extended region of difference 1 (RD1) locus. In this study, a novel locus required for ESX-1 export in M. marinum was identified outside the RD1 locus. An M. marinum strain bearing a transposon-insertion between the MMAR_1663 and MMAR_1664 genes exhibited smooth-colony morphology, was deficient for ESX-1 export, was nonhemolytic, and was attenuated for virulence. Genetic complementation revealed a restoration of colony morphology and a partial restoration of virulence in cell culture models. Yet hemolysis and the export of ESX-1 substrates into the bacteriological medium in vitro as measured by both immunoblotting and quantitative proteomics were not restored. We show that genetic complementation of the transposon insertion strain partially restored the translocation of EsxA and EsxB to the mycobacterial cell surface. Our findings indicate that the export of EsxA and EsxB to the cell surface, rather than secretion into the bacteriological medium, correlates with virulence in M. marinum. Together, these findings not only expand the known genetic loci required for ESX-1 secretion in M. marinum but also provide an explanation for the observed disparity between in vitro ESX-1 export and virulence. PMID:24610712

  11. 75 FR 12433 - National Export Initiative

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-16

    ... Order 13534 of March 11, 2010 National Export Initiative By the authority vested in me as President by... performance will, in turn, create good high-paying jobs. The National Export Initiative (NEI) shall be an Administration initiative to improve conditions that directly affect the private sector's ability to export. The...

  12. 36 CFR 223.87 - Requirements of bidders concerning exports.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Requirements of bidders concerning exports. 223.87 Section 223.87 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER, SPECIAL FOREST PRODUCTS, AND FOREST BOTANICAL...

  13. 36 CFR 223.87 - Requirements of bidders concerning exports.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Requirements of bidders concerning exports. 223.87 Section 223.87 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER, SPECIAL FOREST PRODUCTS, AND FOREST BOTANICAL...

  14. 36 CFR 223.87 - Requirements of bidders concerning exports.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Requirements of bidders concerning exports. 223.87 Section 223.87 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE SALE AND DISPOSAL OF NATIONAL FOREST SYSTEM TIMBER, SPECIAL FOREST PRODUCTS, AND FOREST BOTANICAL...

  15. 7 CFR 20.6 - Submission of reports.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... FAS-100, “Report of Exports for Exporter's Own Account”), setting forth the following information and that required by such forms. Information for each applicable item on the respective form shall be... information contained in the report forms by the use of FAX, telephone, or electronic submission. The required...

  16. 22 CFR 125.6 - Certification requirements for exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... LICENSES FOR THE EXPORT OF TECHNICAL DATA AND CLASSIFIED DEFENSE ARTICLES § 125.6 Certification requirements for exemptions. (a) To claim an exemption for the export of technical data under the provisions of.... Certifications consist of clearly marking the package or letter containing the technical data “22 CFR [insert...

  17. 27 CFR 28.250 - Bills of lading required.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Bills of lading required. 28.250 Section 28.250 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS EXPORTATION OF ALCOHOL Shipment or Delivery for Export Bills of Lading...

  18. 27 CFR 28.250 - Bills of lading required.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Bills of lading required. 28.250 Section 28.250 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS EXPORTATION OF ALCOHOL Shipment or Delivery for Export Bills of Lading...

  19. 27 CFR 28.250 - Bills of lading required.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Bills of lading required. 28.250 Section 28.250 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL EXPORTATION OF ALCOHOL Shipment or Delivery for Export Bills of Lading...

  20. 27 CFR 28.250 - Bills of lading required.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Bills of lading required. 28.250 Section 28.250 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL EXPORTATION OF ALCOHOL Shipment or Delivery for Export Bills of Lading...

  1. 9 CFR 351.16 - Certificate required for shipments of technical animal fat.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... technical animal fat. 351.16 Section 351.16 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... AND VOLUNTARY INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.16 Certificate required for shipments of...

  2. 9 CFR 351.16 - Certificate required for shipments of technical animal fat.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... technical animal fat. 351.16 Section 351.16 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... AND VOLUNTARY INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.16 Certificate required for shipments of...

  3. 9 CFR 351.16 - Certificate required for shipments of technical animal fat.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... technical animal fat. 351.16 Section 351.16 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... AND VOLUNTARY INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.16 Certificate required for shipments of...

  4. 9 CFR 351.16 - Certificate required for shipments of technical animal fat.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... technical animal fat. 351.16 Section 351.16 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... AND VOLUNTARY INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.16 Certificate required for shipments of...

  5. 9 CFR 351.16 - Certificate required for shipments of technical animal fat.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... technical animal fat. 351.16 Section 351.16 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE... AND VOLUNTARY INSPECTION AND CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Transportation and Exportation of Certified Technical Animal Fat § 351.16 Certificate required for shipments of...

  6. 10 CFR 110.54 - Reporting requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... by § 110.26(a) shall submit by February 1 of each year one copy of a report of all components shipped... Terms and Related Provisions § 110.54 Reporting requirements. (a)(1) Reports of exports of nuclear... previous quarter must be submitted by licensees making exports under the general license or specific...

  7. 50 CFR 300.183 - Permit holder reporting and recordkeeping requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... information for each consignment of fish or fish products regulated under this subpart, except shark fins... biweekly report of entries for consumption, exports and re-exports of fish and fish products regulated under this subpart except shark fins. (1) The report required to be submitted under this paragraph (a...

  8. 7 CFR 20.8 - Failure to report.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Failure to report. 20.8 Section 20.8 Agriculture Office of the Secretary of Agriculture EXPORT SALES REPORTING REQUIREMENTS § 20.8 Failure to report. Any person who knowingly fails to report export sales pursuant to the requirements of these regulations shall...

  9. 7 CFR 20.8 - Failure to report.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Failure to report. 20.8 Section 20.8 Agriculture Office of the Secretary of Agriculture EXPORT SALES REPORTING REQUIREMENTS § 20.8 Failure to report. Any person who knowingly fails to report export sales pursuant to the requirements of these regulations shall...

  10. 7 CFR 20.8 - Failure to report.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Failure to report. 20.8 Section 20.8 Agriculture Office of the Secretary of Agriculture EXPORT SALES REPORTING REQUIREMENTS § 20.8 Failure to report. Any person who knowingly fails to report export sales pursuant to the requirements of these regulations shall...

  11. 7 CFR 20.8 - Failure to report.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Failure to report. 20.8 Section 20.8 Agriculture Office of the Secretary of Agriculture EXPORT SALES REPORTING REQUIREMENTS § 20.8 Failure to report. Any person who knowingly fails to report export sales pursuant to the requirements of these regulations shall...

  12. 7 CFR 20.8 - Failure to report.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Failure to report. 20.8 Section 20.8 Agriculture Office of the Secretary of Agriculture EXPORT SALES REPORTING REQUIREMENTS § 20.8 Failure to report. Any person who knowingly fails to report export sales pursuant to the requirements of these regulations shall...

  13. 22 CFR 125.6 - Certification requirements for exemptions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... Certifications consist of clearly marking the package or letter containing the technical data “22 CFR [insert... LICENSES FOR THE EXPORT OF TECHNICAL DATA AND CLASSIFIED DEFENSE ARTICLES § 125.6 Certification requirements for exemptions. (a) To claim an exemption for the export of technical data under the provisions of...

  14. 22 CFR 125.6 - Certification requirements for exemptions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Certifications consist of clearly marking the package or letter containing the technical data “22 CFR [insert... LICENSES FOR THE EXPORT OF TECHNICAL DATA AND CLASSIFIED DEFENSE ARTICLES § 125.6 Certification requirements for exemptions. (a) To claim an exemption for the export of technical data under the provisions of...

  15. 22 CFR 125.6 - Certification requirements for exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... Certifications consist of clearly marking the package or letter containing the technical data “22 CFR [insert... LICENSES FOR THE EXPORT OF TECHNICAL DATA AND CLASSIFIED DEFENSE ARTICLES § 125.6 Certification requirements for exemptions. (a) To claim an exemption for the export of technical data under the provisions of...

  16. 22 CFR 125.6 - Certification requirements for exemptions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... Certifications consist of clearly marking the package or letter containing the technical data “22 CFR [insert... LICENSES FOR THE EXPORT OF TECHNICAL DATA AND CLASSIFIED DEFENSE ARTICLES § 125.6 Certification requirements for exemptions. (a) To claim an exemption for the export of technical data under the provisions of...

  17. Export Airworthiness Approval Procedures

    DOT National Transportation Integrated Search

    1996-05-20

    This advisory circular (AC) contains guidance and information on procedures for : exporting aeronautical products and related special requirements submitted to the Federal Aviation Administration (FAA) by other governments.

  18. 19 CFR 4.75 - Incomplete manifest; incomplete export declarations; bond.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 1302-A (see § 4.63) in accordance with 46 U.S.C. 91, or all required shipper's export declarations (see... declarations; bond. 4.75 Section 4.75 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND... export declarations have been filed with the port director: Albania Bulgaria Cambodia China, People's...

  19. 19 CFR 4.75 - Incomplete manifest; incomplete export declarations; bond.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 1302-A (see § 4.63) in accordance with 46 U.S.C. 91, or all required shipper's export declarations (see... declarations; bond. 4.75 Section 4.75 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND... export declarations have been filed with the port director: Albania Bulgaria Cambodia China, People's...

  20. 19 CFR 4.75 - Incomplete manifest; incomplete export declarations; bond.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 1302-A (see § 4.63) in accordance with 46 U.S.C. 91, or all required shipper's export declarations (see... declarations; bond. 4.75 Section 4.75 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND... export declarations have been filed with the port director: Albania Bulgaria Cambodia China, People's...

  1. 19 CFR 4.75 - Incomplete manifest; incomplete export declarations; bond.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 1302-A (see § 4.63) in accordance with 46 U.S.C. 91, or all required shipper's export declarations (see... declarations; bond. 4.75 Section 4.75 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND... export declarations have been filed with the port director: Albania Bulgaria Cambodia China, People's...

  2. 75 FR 75677 - Agency Information Collection Activities; Proposed Collection; Comment Request; Exports...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-06

    ... requirements for persons exporting human drugs, biological products, devices, animal drugs, food, and cosmetics... Control Number 0910-0482)--Extension The respondents to this information collection are exporters who have... States as allowed under section 801(e) of the Federal Food, Drug, and Cosmetic Act (the FD&C Act) (21 U.S...

  3. 77 FR 3159 - Electronic Export Application and Certification Charge; Flexibility in the Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-23

    ... Marks, Devices, and Certificates; Egg Products Export Certification AGENCY: Food Safety and Inspection... inspection marks, devices, and certificates. In addition, FSIS is proposing to amend the egg product export... possible under the current system. The Egg Products Inspection Act (EPIA) (21 U.S.C. 1031-1056) does not...

  4. 27 CFR 28.316 - Notice to exporter.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... unlawfully diverted, or had been lost by theft, he will advise the exporter by letter: (a) Of the identity of the containers; (b) Of the amount of the loss; (c) Of the circumstances indicating diversion or theft... described in § 28.315, whether by theft or otherwise, the appropriate TTB officer may require the exporter...

  5. 27 CFR 28.316 - Notice to exporter.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... unlawfully diverted, or had been lost by theft, he will advise the exporter by letter: (a) Of the identity of the containers; (b) Of the amount of the loss; (c) Of the circumstances indicating diversion or theft... described in § 28.315, whether by theft or otherwise, the appropriate TTB officer may require the exporter...

  6. 27 CFR 28.103 - Export marks.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Export marks. 28.103... Manufacturing Bonded Warehouse § 28.103 Export marks. (a) General. In addition to the marks and brands required... provisions of part 19 of this chapter, the proprietor shall mark the word “Export” on the Government side of...

  7. Natural gas imports and exports. Second quarter report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1997-12-31

    The Office of Natural Gas and Petroleum Import and Export Activities prepares quarterly reports summarizing the data provided by companies authorized to import or export natural gas. Companies are required, as a condition of their authorizations, to file quarterly reports. This report is for the second quarter of 1997 (April through June).

  8. 50 CFR 21.21 - Import and export permits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... International Trade in Endangered Species of Wild Fauna and Flora (CITES)). (b) Exception to the import permit... bird or birds in, and exported them from, the country of origin. This evidence must include a hunting license and any export documentation required by the country of origin. You must keep these documents with...

  9. 50 CFR 21.21 - Import and export permits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... International Trade in Endangered Species of Wild Fauna and Flora (CITES)). (b) Exception to the import permit... bird or birds in, and exported them from, the country of origin. This evidence must include a hunting license and any export documentation required by the country of origin. You must keep these documents with...

  10. 50 CFR 21.21 - Import and export permits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... International Trade in Endangered Species of Wild Fauna and Flora (CITES)). (b) Exception to the import permit... bird or birds in, and exported them from, the country of origin. This evidence must include a hunting license and any export documentation required by the country of origin. You must keep these documents with...

  11. 78 FR 16365 - Foreign Trade Regulations: Mandatory Automated Export System Filing for All Shipments Requiring...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-14

    ... collecting, compiling, and publishing export trade statistics for the United States under the provisions of... for collecting export trade data, which is used by the Census Bureau for statistical purposes only... timely statistics as well as address the enforcement and security concerns of CBP, the Bureau of Industry...

  12. Tools for Requirements Management: A Comparison of Telelogic DOORS and the HiVe

    DTIC Science & Technology

    2006-07-01

    types DOORS deals with are text files, spreadsheets, FrameMaker , rich text, Microsoft Word and Microsoft Project. 2.5.1 Predefined file formats DOORS...during the export. DOORS exports FrameMaker files in an incomplete format, meaning DOORS exported files will have to be opened in FrameMaker and saved

  13. 50 CFR 23.71 - How can I trade internationally in sturgeon caviar?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...-export sturgeon caviar only if labels are affixed to containers prior to export or re-export in... container. In the United States, the design of the label will be determined by the labeler in accordance with the requirements of this section. (ii) Primary container means any container (tin, jar, pail or...

  14. 15 CFR 758.1 - The Automated Export System (AES) record.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... entered on the loading document (e.g., Cargo Declaration, manifest, bill of lading, (master) air waybill... for all items being exported under the NLR provisions that have a reason for control other than anti-terrorism (AT). The designator “TSPA” may be used, but is not required, when the export consists of...

  15. A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle and Nucleoporin Mutants

    PubMed Central

    Hurt, Ed; Hannus, Stefan; Schmelzl, Birgit; Lau, Denise; Tollervey, David; Simos, George

    1999-01-01

    To identify components involved in the nuclear export of ribosomes in yeast, we developed an in vivo assay exploiting a green fluorescent protein (GFP)-tagged version of ribosomal protein L25. After its import into the nucleolus, L25-GFP assembles with 60S ribosomal subunits that are subsequently exported into the cytoplasm. In wild-type cells, GFP-labeled ribosomes are only detected by fluorescence in the cytoplasm. However, thermosensitive rna1-1 (Ran-GAP), prp20-1 (Ran-GEF), and nucleoporin nup49 and nsp1 mutants are impaired in ribosomal export as revealed by nuclear accumulation of L25-GFP. Furthermore, overexpression of dominant-negative RanGTP (Gsp1-G21V) and the tRNA exportin Los1p inhibits ribosomal export. The pattern of subnuclear accumulation of L25-GFP observed in different mutants is not identical, suggesting that transport can be blocked at different steps. Thus, nuclear export of ribosomes requires the nuclear/cytoplasmic Ran-cycle and distinct nucleoporins. This assay can be used to identify soluble transport factors required for nuclear exit of ribosomes. PMID:9971735

  16. KLF4 Nuclear Export Requires ERK Activation and Initiates Exit from Naive Pluripotency.

    PubMed

    Dhaliwal, Navroop K; Miri, Kamelia; Davidson, Scott; Tamim El Jarkass, Hala; Mitchell, Jennifer A

    2018-04-10

    Cooperative action of a transcription factor complex containing OCT4, SOX2, NANOG, and KLF4 maintains the naive pluripotent state; however, less is known about the mechanisms that disrupt this complex, initiating exit from pluripotency. We show that, as embryonic stem cells (ESCs) exit pluripotency, KLF4 protein is exported from the nucleus causing rapid decline in Nanog and Klf4 transcription; as a result, KLF4 is the first pluripotency transcription factor removed from transcription-associated complexes during differentiation. KLF4 nuclear export requires ERK activation, and phosphorylation of KLF4 by ERK initiates interaction of KLF4 with nuclear export factor XPO1, leading to KLF4 export. Mutation of the ERK phosphorylation site in KLF4 (S132) blocks KLF4 nuclear export, the decline in Nanog, Klf4, and Sox2 mRNA, and differentiation. These findings demonstrate that relocalization of KLF4 to the cytoplasm is a critical first step in exit from the naive pluripotent state and initiation of ESC differentiation. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

  17. A translocator-specific export signal establishes the translocator-effector secretion hierarchy that is important for type III secretion system function

    PubMed Central

    Tomalka, Amanda G.; Stopford, Charles M.; Lee, Pei-Chung; Rietsch, Arne

    2012-01-01

    Summary Type III secretion systems are used by many Gram-negative pathogens to directly deliver effector proteins into the cytoplasm of host cells. To accomplish this, bacteria secrete translocator proteins that form a pore in the host-cell membrane through which the effector proteins are then introduced into the host cell. Evidence from multiple systems indicates that the pore-forming translocator proteins are exported before effectors, but how this secretion hierarchy is established is unclear. Here we used the P. aeruginosa translocator protein PopD as a model to identify its export signals. The amino-terminal secretion signal and chaperone, PcrH, are required for export under all conditions. Two novel signals in PopD, one proximal to the chaperone-binding site and one at the very C-terminus of the protein, are required for export of PopD before effector proteins. These novel export signals establish the translocator-effector secretion hierarchy, which in turn, is critical for the delivery of effectors into host cells. PMID:23121689

  18. 15 CFR 30.45 - General statement of requirements for the filing of carrier manifests with proof of filing...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false General statement of requirements for the filing of carrier manifests with proof of filing citations for the electronic submission of export information or exemption legends when Electronic Export Information filing is not required. 30.45 Section 30.45 Commerce and Foreign Trade...

  19. 7 CFR 1493.490 - Proof of entry.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program... translation acceptable to CCC. Records acceptable to meet this requirement include an original certification...

  20. 7 CFR 1493.490 - Proof of entry.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program... translation acceptable to CCC. Records acceptable to meet this requirement include an original certification...

  1. 7 CFR 1493.490 - Proof of entry.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program... translation acceptable to CCC. Records acceptable to meet this requirement include an original certification...

  2. SR proteins SRp20 and 9G8 contribute to efficient export of herpes simplex virus 1 mRNAs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Escudero-Paunetto, Laurimar; Li Ling; Hernandez, Felicia P.

    2010-06-05

    Herpes simplex virus 1 (HSV-1) mRNAs are exported to the cytoplasm through the export receptor TAP/NFX1. HSV-1 multifunctional protein ICP27 interacts with TAP/NXF1, binds viral RNAs, and is required for efficient viral RNA export. In ICP27 mutant infections, viral RNA export is reduced but not ablated, indicating that other export adaptors can aid in viral RNA export. Export adaptor protein Aly/REF is recruited to viral replication compartments, however, Aly/REF knockdown has little effect on viral RNA export. SR proteins SRp20 and 9G8 interact with TAP/NXF1 and mediate export of some cellular RNAs. We report that siRNA knockdown of SRp20 ormore » 9G8 resulted in about a 10 fold decrease in virus yields and in nuclear accumulation of poly(A+) RNA. In infected cells depleted of SRp20, newly transcribed Bromouridine-labeled RNA also accumulated in the nucleus. We conclude that SRp20 and 9G8 contribute to HSV-1 RNA export.« less

  3. 76 FR 41860 - Information Collection Available for Public Comments and Recommendations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-15

    ... Shipments Moving Under Export-Import Bank Financing. Type of Request: Extension of currently approved...: 46 App. U.S.C. 1241-1, Public Resolution 17, required MARAD to monitor and enforce the U.S.-flag shipping requirements relative to the loans/guarantees extended by the Export-Import Bank (EXIMBANK) to...

  4. 19 CFR 191.42 - Procedure.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... to exportations under 19 U.S.C. 1313(c). (d) Required Information. The notice shall provide the bill... required in paragraph (b)(1) of this section. (c) Notice. A notice of intent to export or destroy merchandise which may be the subject of a rejected merchandise drawback claim (19 U.S.C. 1313(c)) must be...

  5. 19 CFR 191.42 - Procedure.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... to exportations under 19 U.S.C. 1313(c). (d) Required Information. The notice shall provide the bill... required in paragraph (b)(1) of this section. (c) Notice. A notice of intent to export or destroy merchandise which may be the subject of a rejected merchandise drawback claim (19 U.S.C. 1313(c)) must be...

  6. 19 CFR 191.42 - Procedure.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... to exportations under 19 U.S.C. 1313(c). (d) Required Information. The notice shall provide the bill... required in paragraph (b)(1) of this section. (c) Notice. A notice of intent to export or destroy merchandise which may be the subject of a rejected merchandise drawback claim (19 U.S.C. 1313(c)) must be...

  7. 19 CFR 191.42 - Procedure.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... to exportations under 19 U.S.C. 1313(c). (d) Required Information. The notice shall provide the bill... required in paragraph (b)(1) of this section. (c) Notice. A notice of intent to export or destroy merchandise which may be the subject of a rejected merchandise drawback claim (19 U.S.C. 1313(c)) must be...

  8. RNA Export through the NPC in Eukaryotes.

    PubMed

    Okamura, Masumi; Inose, Haruko; Masuda, Seiji

    2015-03-20

    In eukaryotic cells, RNAs are transcribed in the nucleus and exported to the cytoplasm through the nuclear pore complex. The RNA molecules that are exported from the nucleus into the cytoplasm include messenger RNAs (mRNAs), ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), small nuclear RNAs (snRNAs), micro RNAs (miRNAs), and viral mRNAs. Each RNA is transported by a specific nuclear export receptor. It is believed that most of the mRNAs are exported by Nxf1 (Mex67 in yeast), whereas rRNAs, snRNAs, and a certain subset of mRNAs are exported in a Crm1/Xpo1-dependent manner. tRNAs and miRNAs are exported by Xpot and Xpo5. However, multiple export receptors are involved in the export of some RNAs, such as 60S ribosomal subunit. In addition to these export receptors, some adapter proteins are required to export RNAs. The RNA export system of eukaryotic cells is also used by several types of RNA virus that depend on the machineries of the host cell in the nucleus for replication of their genome, therefore this review describes the RNA export system of two representative viruses. We also discuss the NPC anchoring-dependent mRNA export factors that directly recruit specific genes to the NPC.

  9. RNA Export through the NPC in Eukaryotes

    PubMed Central

    Okamura, Masumi; Inose, Haruko; Masuda, Seiji

    2015-01-01

    In eukaryotic cells, RNAs are transcribed in the nucleus and exported to the cytoplasm through the nuclear pore complex. The RNA molecules that are exported from the nucleus into the cytoplasm include messenger RNAs (mRNAs), ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), small nuclear RNAs (snRNAs), micro RNAs (miRNAs), and viral mRNAs. Each RNA is transported by a specific nuclear export receptor. It is believed that most of the mRNAs are exported by Nxf1 (Mex67 in yeast), whereas rRNAs, snRNAs, and a certain subset of mRNAs are exported in a Crm1/Xpo1-dependent manner. tRNAs and miRNAs are exported by Xpot and Xpo5. However, multiple export receptors are involved in the export of some RNAs, such as 60S ribosomal subunit. In addition to these export receptors, some adapter proteins are required to export RNAs. The RNA export system of eukaryotic cells is also used by several types of RNA virus that depend on the machineries of the host cell in the nucleus for replication of their genome, therefore this review describes the RNA export system of two representative viruses. We also discuss the NPC anchoring-dependent mRNA export factors that directly recruit specific genes to the NPC. PMID:25802992

  10. 22 CFR 122.4 - Notification of changes in information furnished by registrants.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... export or temporarily import defense articles or defense services from any agency of the U.S. government... obtaining the approval required under this subchapter for the export of defense articles or defense services... authority of § 38(g)(6) of the Arms Export Control Act regarding licenses or other approvals for certain...

  11. 10 CFR 75.45 - Content of advance notification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... information is being supplied under § 75.43. (b) The notifications required with respect to export and import... export license issued pursuant to part 110 of this chapter, or origin of import (by country and, if known... (for exports) or in the United States (for imports). [45 FR 50711, July 31, 1980, as amended at 73 FR...

  12. 10 CFR 75.45 - Content of advance notification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... information is being supplied under § 75.43. (b) The notifications required with respect to export and import... export license issued pursuant to part 110 of this chapter, or origin of import (by country and, if known... (for exports) or in the United States (for imports). [45 FR 50711, July 31, 1980, as amended at 73 FR...

  13. 22 CFR 122.4 - Notification of changes in information furnished by registrants.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... export or temporarily import defense articles or defense services from any agency of the U.S. government... obtaining the approval required under this subchapter for the export of defense articles or defense services... authority of § 38(g)(6) of the Arms Export Control Act regarding licenses or other approvals for certain...

  14. 7 CFR 51.883 - U.S. Fancy Export.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false U.S. Fancy Export. 51.883 Section 51.883 Agriculture... Standards for Grades of Table Grapes (European or Vinifera Type) 1 Grades § 51.883 U.S. Fancy Export. “U.S. Fancy Export” consists of grapes which meet the requirements for U.S. Fancy Table, except that bunches...

  15. 9 CFR 113.10 - Testing of bulk material for export or for further manufacture.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Testing of bulk material for export or for further manufacture. 113.10 Section 113.10 Animals and Animal Products ANIMAL AND PLANT HEALTH... VECTORS STANDARD REQUIREMENTS Applicability § 113.10 Testing of bulk material for export or for further...

  16. 9 CFR 113.10 - Testing of bulk material for export or for further manufacture.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Testing of bulk material for export or for further manufacture. 113.10 Section 113.10 Animals and Animal Products ANIMAL AND PLANT HEALTH... VECTORS STANDARD REQUIREMENTS Applicability § 113.10 Testing of bulk material for export or for further...

  17. 9 CFR 113.10 - Testing of bulk material for export or for further manufacture.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Testing of bulk material for export or for further manufacture. 113.10 Section 113.10 Animals and Animal Products ANIMAL AND PLANT HEALTH... VECTORS STANDARD REQUIREMENTS Applicability § 113.10 Testing of bulk material for export or for further...

  18. 9 CFR 113.10 - Testing of bulk material for export or for further manufacture.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Testing of bulk material for export or for further manufacture. 113.10 Section 113.10 Animals and Animal Products ANIMAL AND PLANT HEALTH... VECTORS STANDARD REQUIREMENTS Applicability § 113.10 Testing of bulk material for export or for further...

  19. 9 CFR 113.10 - Testing of bulk material for export or for further manufacture.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Testing of bulk material for export or for further manufacture. 113.10 Section 113.10 Animals and Animal Products ANIMAL AND PLANT HEALTH... VECTORS STANDARD REQUIREMENTS Applicability § 113.10 Testing of bulk material for export or for further...

  20. 7 CFR 1488.9a - Evidence of export for commodities delivered before export.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required for Financing § 1488.9a Evidence... certification to the Treasurer, CCC, within 60 days from the date of delivery or such extension of time as may be granted by the Treasurer or Assistant Treasurer, CCC, certifying that the commodities have been...

  1. 7 CFR 1488.9a - Evidence of export for commodities delivered before export.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Stocks Under CCC Export Credit Sales Program (GSM-5) Documents Required for Financing § 1488.9a Evidence... certification to the Treasurer, CCC, within 60 days from the date of delivery or such extension of time as may be granted by the Treasurer or Assistant Treasurer, CCC, certifying that the commodities have been...

  2. A Herpesvirus Protein Selectively Inhibits Cellular mRNA Nuclear Export.

    PubMed

    Gong, Danyang; Kim, Yong Hoon; Xiao, Yuchen; Du, Yushen; Xie, Yafang; Lee, Kevin K; Feng, Jun; Farhat, Nisar; Zhao, Dawei; Shu, Sara; Dai, Xinghong; Chanda, Sumit K; Rana, Tariq M; Krogan, Nevan J; Sun, Ren; Wu, Ting-Ting

    2016-11-09

    Nuclear mRNA export is highly regulated to ensure accurate cellular gene expression. Viral inhibition of cellular mRNA export can enhance viral access to the cellular translation machinery and prevent anti-viral protein production but is generally thought to be nonselective. We report that ORF10 of Kaposi's sarcoma-associated herpesvirus (KSHV), a nuclear DNA virus, inhibits mRNA export in a transcript-selective manner to control cellular gene expression. Nuclear export inhibition by ORF10 requires an interaction with an RNA export factor, Rae1. Genome-wide analysis reveals a subset of cellular mRNAs whose nuclear export is blocked by ORF10 with the 3' UTRs of ORF10-targeted transcripts conferring sensitivity to export inhibition. The ORF10-Rae1 interaction is important for the virus to express viral genes and produce infectious virions. These results suggest that a nuclear DNA virus can selectively interfere with RNA export to restrict host gene expression for optimal replication. Published by Elsevier Inc.

  3. 50 CFR 14.21 - Shellfish and fishery products.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... PLANTS IMPORTATION, EXPORTATION, AND TRANSPORTATION OF WILDLIFE Importation and Exportation at Designated... wildlife requiring a permit pursuant to part 17 or part 23 of this subchapter, live aquatic invertebrates...

  4. 50 CFR 14.21 - Shellfish and fishery products.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PLANTS IMPORTATION, EXPORTATION, AND TRANSPORTATION OF WILDLIFE Importation and Exportation at Designated... wildlife requiring a permit pursuant to part 17 or part 23 of this subchapter, live aquatic invertebrates...

  5. Yeast Ran-Binding Protein 1 (Yrb1) Shuttles between the Nucleus and Cytoplasm and Is Exported from the Nucleus via a CRM1 (XPO1)-Dependent Pathway

    PubMed Central

    Künzler, Markus; Gerstberger, Thomas; Stutz, Françoise; Bischoff, F. Ralf; Hurt, Ed

    2000-01-01

    The RanGTP-binding protein RanBP1, which is located in the cytoplasm, has been implicated in release of nuclear export complexes from the cytoplasmic side of the nuclear pore complex. Here we show that Yrb1 (the yeast homolog of RanBP1) shuttles between the nucleus and the cytoplasm. Nuclear import of Yrb1 is a facilitated process that requires a short basic sequence within the Ran-binding domain (RBD). By contrast, nuclear export of Yrb1 requires an intact RBD, which forms a ternary complex with the Xpo1 (Crm1) NES receptor in the presence of RanGTP. Nuclear export of Yrb1, however, is insensitive towards leptomycin B, suggesting a novel type of substrate recognition between Yrb1 and Xpo1. Taken together, these data suggest that ongoing nuclear import and export is an important feature of Yrb1 function in vivo. PMID:10825193

  6. 77 FR 71213 - Revisions to the Export Administration Regulations (EAR) To Make the Commerce Control List (CCL...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-29

    ...On December 9, 2010, the Bureau of Industry and Security (BIS) published an advance notice of proposed rulemaking entitled Commerce Control List: Revising Descriptions of Items and Foreign Availability as part of the President's Export Control Reform (ECR) Initiative. The December 9, 2010 notice sought, among other things, public comments on how descriptions of items controlled on the Commerce Control List (CCL) could be made clearer. This proposed rule would implement changes identified by BIS and the public that would make the CCL clearer. This rule would only implement changes that can be made to the CCL without requiring changes to multilateral export control regime guidelines or lists. However, BIS has identified changes that would require a decision of a multilateral regime to implement. For those changes, the U.S. Government is developing regime change proposals for consideration by members of those multilateral export control regimes. BIS will implement those changes in separate rulemakings, if approved by the respective multilateral export control regimes.

  7. Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export

    PubMed Central

    2018-01-01

    The bacterial flagellum is a supramolecular motility machine. Flagellar assembly begins with the basal body, followed by the hook and finally the filament. A carboxyl-terminal cytoplasmic domain of FlhA (FlhAC) forms a nonameric ring structure in the flagellar type III protein export apparatus and coordinates flagellar protein export with assembly. However, the mechanism of this process remains unknown. We report that a flexible linker of FlhAC (FlhAL) is required not only for FlhAC ring formation but also for substrate specificity switching of the protein export apparatus from the hook protein to the filament protein upon completion of the hook structure. FlhAL was required for cooperative ring formation of FlhAC. Alanine substitutions of residues involved in FlhAC ring formation interfered with the substrate specificity switching, thereby inhibiting filament assembly at the hook tip. These observations lead us to propose a mechanistic model for export switching involving structural remodeling of FlhAC. PMID:29707633

  8. Measuring the globalization of cities from the new regionalism perspective.

    PubMed

    Ergüzel, Oylum Şehvez; Tunahan, Hakan; Esen, Sinan

    2016-01-01

    The study aims to analyze the export performance of countries and of cities within them to identify synchronized or unsynchronized movement between them. In the empirical part of the study, the measurements used to analyze the export performance of the countries included in the literature are applied to establish the export performance of a single city-Sakarya, Turkey. These measurements include the Herfindahl-Hirchman product and market concentration indices, the Lawrence index, the trade complementarity index, and the Grubel-Lloyd intra-industry index, as well as additional indicators with local or regional contexts. The limited number of studies analyzing the export competitiveness of a single city with relevant formats in the literature reveal the significance of the study.

  9. Current food safety management systems in fish-exporting companies require further improvements to adequately cope with contextual pressure: case study.

    PubMed

    Onjong, Hillary Adawo; Wangoh, John; Njage, Patrick Murigu Kamau

    2014-10-01

    Fish-processing plants still face food safety (FS) challenges worldwide despite the existence of several quality assurance standards and food safety management systems/s (FSMSs). This study assessed performance of FSMS in fish exporting sector considering pressure from the context in which they operate. A FSMS diagnostic tool with checklist was used to assess the context, FSMS, and FS output in 9 Kenyan fish exporting companies. Majority (67%) companies operated at moderate- to high-risk context but with an average performance in control and assurance activities. This situation could be insufficient to deal with ambiguity, uncertainty, and vulnerability issues in the context characteristics. Contextual risk posed by product characteristics (nature of raw materials) and chain environment characteristics was high. Risk posed by the chain environment characteristics, low power in supplier relationships, and low degree of authority in customer relationships was high. Lack of authority in relationship with suppliers would lead to high raw material risk situation. Even though cooling facilities, a key control activity, was at an advanced level, there was inadequate packaging intervention equipment which coupled with inadequate physical intervention equipment could lead to further weakened FSMS performance. For the fish companies to improve their FSMS to higher level and enhance predictability, they should base their FSMS on scientific information sources, historical results, and own experimental trials in their preventive, intervention, and monitoring systems. Specific suggestions are derived for improvements toward higher FSMS activity levels or lower risk levels in context characteristics. Weak areas in performance of control and assurance activities in export fish-processing sector already implementing current quality assurance guidelines and standards were studied taking into consideration contextual pressure wherein the companies operate. Important mitigation measures toward improved contextual risk, core assurance, and control activities irrespective of applied food safety management systems in fish industries were suggested. © 2014 Institute of Food Technologists®

  10. Export of l-Isoleucine from Corynebacterium glutamicum: a Two-Gene-Encoded Member of a New Translocator Family

    PubMed Central

    Kennerknecht, Nicole; Sahm, Hermann; Yen, Ming-Ren; Pátek, Miroslav; Saier, Jr., Milton H.; Eggeling, Lothar

    2002-01-01

    Bacteria possess amino acid export systems, and Corynebacterium glutamicum excretes l-isoleucine in a process dependent on the proton motive force. In order to identify the system responsible for l-isoleucine export, we have used transposon mutagenesis to isolate mutants of C. glutamicum sensitive to the peptide isoleucyl-isoleucine. In one such mutant, strong peptide sensitivity resulted from insertion into a gene designated brnF encoding a hydrophobic protein predicted to possess seven transmembrane spanning helices. brnE is located downstream of brnF and encodes a second hydrophobic protein with four putative membrane-spanning helices. A mutant deleted of both genes no longer exports l-isoleucine, whereas an overexpressing strain exports this amino acid at an increased rate. BrnF and BrnE together are also required for the export of l-leucine and l-valine. BrnFE is thus a two-component export permease specific for aliphatic hydrophobic amino acids. Upstream of brnFE and transcribed divergently is an Lrp-like regulatory gene required for active export. Searches for homologues of BrnFE show that this type of exporter is widespread in prokaryotes but lacking in eukaryotes and that both gene products which together comprise the members of a novel family, the LIV-E family, generally map together within a single operon. Comparisons of the BrnF and BrnE phylogenetic trees show that gene duplication events in the early bacterial lineage gave rise to multiple paralogues that have been retained in α-proteobacteria but not in other prokaryotes analyzed. PMID:12081967

  11. 7 CFR 33.10 - Minimum requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ISSUED UNDER AUTHORITY OF THE EXPORT APPLE ACT Regulations § 33.10 Minimum requirements. No person shall... shipment of apples to any foreign destination unless: (a) Apples grade at least U.S. No. 1 or U.S. No. 1 Early: Provided, That apples for export to Pacific ports of Russia shall grade at least U.S. Utility or...

  12. 7 CFR 33.10 - Minimum requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... ISSUED UNDER AUTHORITY OF THE EXPORT APPLE ACT Regulations § 33.10 Minimum requirements. No person shall... shipment of apples to any foreign destination unless: (a) Apples grade at least U.S. No. 1 or U.S. No. 1 Early: Provided, That apples for export to Pacific ports of Russia shall grade at least U.S. Utility or...

  13. 7 CFR 33.10 - Minimum requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... ISSUED UNDER AUTHORITY OF THE EXPORT APPLE ACT Regulations § 33.10 Minimum requirements. No person shall... shipment of apples to any foreign destination unless: (a) Apples grade at least U.S. No. 1 or U.S. No. 1 Early: Provided, That apples for export to Pacific ports of Russia shall grade at least U.S. Utility or...

  14. 7 CFR 33.10 - Minimum requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ISSUED UNDER AUTHORITY OF THE EXPORT APPLE ACT Regulations § 33.10 Minimum requirements. No person shall... shipment of apples to any foreign destination unless: (a) Apples grade at least U.S. No. 1 or U.S. No. 1 Early: Provided, That apples for export to Pacific ports of Russia shall grade at least U.S. Utility or...

  15. 7 CFR 33.10 - Minimum requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ISSUED UNDER AUTHORITY OF THE EXPORT APPLE ACT Regulations § 33.10 Minimum requirements. No person shall... shipment of apples to any foreign destination unless: (a) Apples grade at least U.S. No. 1 or U.S. No. 1 Early: Provided, That apples for export to Pacific ports of Russia shall grade at least U.S. Utility or...

  16. 15 CFR 713.3 - Annual declaration and reporting requirements for exports and imports of Schedule 2 chemicals.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... requirements for exports and imports of Schedule 2 chemicals. 713.3 Section 713.3 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) BUREAU OF INDUSTRY AND SECURITY, DEPARTMENT OF COMMERCE CHEMICAL WEAPONS CONVENTION REGULATIONS ACTIVITIES INVOLVING SCHEDULE 2 CHEMICALS § 713.3 Annual...

  17. 15 CFR 30.10 - Retention of export information and the authority to require production of documents.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Retention of export information and the authority to require production of documents. 30.10 Section 30.10 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade BUREAU OF THE CENSUS, DEPARTMENT OF COMMERCE FOREIGN TRADE...

  18. 77 FR 23114 - Addition of Certain Persons on the Entity List: Addition of Persons Acting Contrary to the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ... exports, reexports, and transfers (in-country) to entities identified on the Entity List require a license... CONTACT: Karen Nies-Vogel, Chair, End-User Review Committee, Office of the Assistant Secretary, Export...) to entities identified on the Entity List require a license from the Bureau of Industry and Security...

  19. 19 CFR 192.14 - Electronic information for outward cargo required in advance of departure.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Information Through the Automated Export System (AES) § 192.14 Electronic information for outward cargo... Automated Export System (AES)). (b) Presentation of data—(1) Time for presenting data. USPPIs or their... regulatory authority to do so. The CBP will also continue to require 72-hour advance notice for used vehicle...

  20. 19 CFR 192.14 - Electronic information for outward cargo required in advance of departure.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Information Through the Automated Export System (AES) § 192.14 Electronic information for outward cargo... Automated Export System (AES)). (b) Presentation of data—(1) Time for presenting data. USPPIs or their... regulatory authority to do so. The CBP will also continue to require 72-hour advance notice for used vehicle...

  1. 19 CFR 192.14 - Electronic information for outward cargo required in advance of departure.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Information Through the Automated Export System (AES) § 192.14 Electronic information for outward cargo... Automated Export System (AES)). (b) Presentation of data—(1) Time for presenting data. USPPIs or their... regulatory authority to do so. The CBP will also continue to require 72-hour advance notice for used vehicle...

  2. 19 CFR 192.14 - Electronic information for outward cargo required in advance of departure.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Information Through the Automated Export System (AES) § 192.14 Electronic information for outward cargo... Automated Export System (AES)). (b) Presentation of data—(1) Time for presenting data. USPPIs or their... regulatory authority to do so. The CBP will also continue to require 72-hour advance notice for used vehicle...

  3. 50 CFR 300.185 - Documentation, reporting and recordkeeping requirements for consignment documents and re-export...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... (3) Reporting requirements. For fish or fish products regulated under this subpart, except shark fins... this paragraph (b) apply to exports of fish or fish products regulated under this subpart, except shark... consignment of fish or fish products regulated under this subpart, except bluefin tuna or shark fins, that was...

  4. 27 CFR 44.145 - Special.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... PAYMENT OF TAX, OR WITH DRAWBACK OF TAX Operations by Export Warehouse Proprietors Inventories § 44.145 Special. A special inventory shall be made by the export warehouse proprietor whenever required by any...

  5. 7 CFR 35.11 - Minimum requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... applicable to the variety and destination specified: (a) Any such variety for export to destinations in Japan... for export to any foreign destination, other than destinations in Japan, Europe, Greenland, Canada, or...

  6. 7 CFR 35.11 - Minimum requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... applicable to the variety and destination specified: (a) Any such variety for export to destinations in Japan... for export to any foreign destination, other than destinations in Japan, Europe, Greenland, Canada, or...

  7. 7 CFR 35.11 - Minimum requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... applicable to the variety and destination specified: (a) Any such variety for export to destinations in Japan... for export to any foreign destination, other than destinations in Japan, Europe, Greenland, Canada, or...

  8. 50 CFR 14.64 - Exceptions to export declaration requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the exportation of live aquatic invertebrates of the Class Pelecypoda (commonly known as oysters... products or manufactured articles, including game trophies, that are not intended for commercial use and...

  9. 7 CFR 1493.6 - Additional required determinations for GSM-103.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS... GSM-103. Notwithstanding any other provision under this part, CCC shall not guarantee under the GSM...

  10. 7 CFR 1493.6 - Additional required determinations for GSM-103.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS... GSM-103. Notwithstanding any other provision under this part, CCC shall not guarantee under the GSM...

  11. 7 CFR 1493.6 - Additional required determinations for GSM-103.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS... GSM-103. Notwithstanding any other provision under this part, CCC shall not guarantee under the GSM...

  12. Bayesian Networks to Compare Pest Control Interventions on Commodities Along Agricultural Production Chains.

    PubMed

    Holt, J; Leach, A W; Johnson, S; Tu, D M; Nhu, D T; Anh, N T; Quinlan, M M; Whittle, P J L; Mengersen, K; Mumford, J D

    2018-02-01

    The production of an agricultural commodity involves a sequence of processes: planting/growing, harvesting, sorting/grading, postharvest treatment, packing, and exporting. A Bayesian network has been developed to represent the level of potential infestation of an agricultural commodity by a specified pest along an agricultural production chain. It reflects the dependency of this infestation on the predicted level of pest challenge, the anticipated susceptibility of the commodity to the pest, the level of impact from pest control measures as designed, and any variation from that due to uncertainty in measure efficacy. The objective of this Bayesian network is to facilitate agreement between national governments of the exporters and importers on a set of phytosanitary measures to meet specific phytosanitary measure requirements to achieve target levels of protection against regulated pests. The model can be used to compare the performance of different combinations of measures under different scenarios of pest challenge, making use of available measure performance data. A case study is presented using a model developed for a fruit fly pest on dragon fruit in Vietnam; the model parameters and results are illustrative and do not imply a particular level of fruit fly infestation of these exports; rather, they provide the most likely, alternative, or worst-case scenarios of the impact of measures. As a means to facilitate agreement for trade, the model provides a framework to support communication between exporters and importers about any differences in perceptions of the risk reduction achieved by pest control measures deployed during the commodity production chain. © 2017 Society for Risk Analysis.

  13. 15 CFR 30.4 - Electronic Export Information filing procedures, deadlines, and certification statements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., and consignees may be known prior to exportation. The procedures for obtaining certification as an AES... authorized agent shall file the required EEI and have received the AES ITN no later than the time period... because the AES is not operating, the filer shall not export until the AES is operating and an ITN is...

  14. 15 CFR 30.4 - Electronic Export Information filing procedures, deadlines, and certification statements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., and consignees may be known prior to exportation. The procedures for obtaining certification as an AES... authorized agent shall file the required EEI and have received the AES ITN no later than the time period... because the AES is not operating, the filer shall not export until the AES is operating and an ITN is...

  15. 15 CFR 30.4 - Electronic Export Information filing procedures, deadlines, and certification statements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., and consignees may be known prior to exportation. The procedures for obtaining certification as an AES... authorized agent shall file the required EEI and have received the AES ITN no later than the time period... because the AES is not operating, the filer shall not export until the AES is operating and an ITN is...

  16. 15 CFR 30.4 - Electronic Export Information filing procedures, deadlines, and certification statements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., and consignees may be known prior to exportation. The procedures for obtaining certification as an AES... authorized agent shall file the required EEI and have received the AES ITN no later than the time period... because the AES is not operating, the filer shall not export until the AES is operating and an ITN is...

  17. 15 CFR 30.4 - Electronic Export Information filing procedures, deadlines, and certification statements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., and consignees may be known prior to exportation. The procedures for obtaining certification as an AES... authorized agent shall file the required EEI and have received the AES ITN no later than the time period... because the AES is not operating, the filer shall not export until the AES is operating and an ITN is...

  18. 15 CFR 713.3 - Annual declaration and reporting requirements for exports and imports of Schedule 2 chemicals.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... reports of exports and imports of Schedule 2 chemicals—(1) Declarations. A Schedule 2 plant site that is declared because it produced, processed or consumed a Schedule 2 chemical at one or more plants above the... plant site that same Schedule 2 chemical above the applicable threshold, must submit export and import...

  19. 15 CFR 713.3 - Annual declaration and reporting requirements for exports and imports of Schedule 2 chemicals.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... reports of exports and imports of Schedule 2 chemicals—(1) Declarations. A Schedule 2 plant site that is declared because it produced, processed or consumed a Schedule 2 chemical at one or more plants above the... plant site that same Schedule 2 chemical above the applicable threshold, must submit export and import...

  20. 15 CFR 713.3 - Annual declaration and reporting requirements for exports and imports of Schedule 2 chemicals.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... reports of exports and imports of Schedule 2 chemicals—(1) Declarations. A Schedule 2 plant site that is declared because it produced, processed or consumed a Schedule 2 chemical at one or more plants above the... plant site that same Schedule 2 chemical above the applicable threshold, must submit export and import...

  1. How to Build an Export Business. An International Marketing Guide for the Minority-Owned Firm.

    ERIC Educational Resources Information Center

    Joyner, Nelson T., Jr.; Lurie, Richard G.

    Designed to aid minority firms who are inexperienced in the export market, this handbook provides a step-by-step guide to the development of an export business, describing what to do, how to do it, and where to find more information on specific international marketing help. Starting with the easiest opportunities that do not require any…

  2. 21 CFR 312.110 - Import and export requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... drug has valid marketing authorization in Australia, Canada, Israel, Japan, New Zealand, Switzerland..., and § 1.101 of this chapter; or (3) The drug is being exported to Australia, Canada, Israel, Japan...

  3. Suppressor Analysis Reveals a Role for SecY in the SecA2-Dependent Protein Export Pathway of Mycobacteria

    PubMed Central

    Ligon, Lauren S.; Rigel, Nathan W.; Romanchuk, Artur; Jones, Corbin D.

    2013-01-01

    All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-dependent pathway exports a small subset of proteins and is required for Mycobacterium tuberculosis virulence. The mechanism by which SecA2 drives export of proteins across the cytoplasmic membrane remains poorly understood. Here we performed suppressor analysis on a dominant negative secA2 mutant (secA2 K129R) of the model mycobacterium Mycobacterium smegmatis to better understand the pathway used by SecA2 to export proteins. Two extragenic suppressor mutations were identified as mapping to the promoter region of secY, which encodes the central component of the canonical Sec export channel. These suppressor mutations increased secY expression, and this effect was sufficient to alleviate the secA2 K129R phenotype. We also discovered that the level of SecY protein was greatly diminished in the secA2 K129R mutant, but at least partially restored in the suppressors. Furthermore, the level of SecY in a suppressor strongly correlated with the degree of suppression. Our findings reveal a detrimental effect of SecA2 K129R on SecY, arguing for an integrated system in which SecA2 works with SecY and the canonical Sec translocase to export proteins. PMID:23913320

  4. Mutational definition of functional domains within the Rev homolog encoded by human endogenous retrovirus K.

    PubMed

    Bogerd, H P; Wiegand, H L; Yang, J; Cullen, B R

    2000-10-01

    Nuclear export of the incompletely spliced mRNAs encoded by several complex retroviruses, including human immunodeficiency virus type 1 (HIV-1), is dependent on a virally encoded adapter protein, termed Rev in HIV-1, that directly binds both to a cis-acting viral RNA target site and to the cellular Crm1 export factor. Human endogenous retrovirus K, a family of ancient endogenous retroviruses that is not related to the exogenous retrovirus HIV-1, was recently shown to also encode a Crm1-dependent nuclear RNA export factor, termed K-Rev. Although HIV-1 Rev and K-Rev display little sequence identity, they share the ability not only to bind to Crm1 and to RNA but also to form homomultimers and shuttle between nucleus and cytoplasm. We have used mutational analysis to identify sequences in the 105-amino-acid K-Rev protein required for each of these distinct biological activities. While mutations in K-Rev that inactivate any one of these properties also blocked K-Rev-dependent nuclear RNA export, several K-Rev mutants were comparable to wild type when assayed for any of these individual activities yet nevertheless defective for RNA export. Although several nonfunctional K-Rev mutants acted as dominant negative inhibitors of K-Rev-, but not HIV-1 Rev-, dependent RNA export, these were not defined by their inability to bind to Crm1, as is seen with HIV-1 Rev. In total, this analysis suggests a functional architecture for K-Rev that is similar to, but distinct from, that described for HIV-1 Rev and raises the possibility that viral RNA export mediated by the approximately 25 million-year-old K-Rev protein may require an additional cellular cofactor that is not required for HIV-1 Rev function.

  5. 7 CFR 987.112a - Grade, size, and container requirements for each outlet category.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... supplies intended strictly for medical purposes. (3) Dates of any variety identified as “Export—Mexico” and inspected and certified as at least meeting the requirements of U.S. Grade C may be exported only to Mexico. No dates shall be exported to Mexico unless the handler certifies to the Committee and the U.S...

  6. Nuclear export of the small ribosomal subunit requires the Ran–GTPase cycle and certain nucleoporins

    PubMed Central

    Moy, Terence I.; Silver, Pamela A.

    1999-01-01

    After their assembly in the nucleolus, ribosomal subunits are exported from the nucleus to the cytoplasm. After export, the 20S rRNA in the small ribosomal subunit is cleaved to yield 18S rRNA and the small 5′ ITS1 fragment. The 5′ ITS1 RNA is normally degraded by the cytoplasmic Xrn1 exonuclease, but in strains lacking XRN1, the 5′ ITS1 fragment accumulates in the cytoplasm. Using the cytoplasmic localization of the 5′ ITS1 fragment as an indicator for the export of the small ribosomal subunit, we have identified genes that are required for ribosome export. Ribosome export is dependent on the Ran–GTPase as mutations in Ran or its regulators caused 5′ ITS1 to accumulate in the nucleoplasm. Mutations in the genes encoding the nucleoporin Nup82 and in the NES exporter Xpo1/Crm1 also caused the nucleoplasmic accumulation of 5′ ITS1. Mutants in a subset of nucleoporins and in the nuclear transport factors Srp1, Kap95, Pse1, Cse1, and Mtr10 accumulate the 5′ ITS1 in the nucleolus and affect ribosome assembly. In contrast, we did not detect nuclear accumulation of 5′ ITS1 in 28 yeast strains that have mutations in other genes affecting nuclear trafficking. PMID:10465789

  7. IRAK2 directs stimulus-dependent nuclear export of inflammatory mRNAs

    PubMed Central

    Yu, Minjia; Qian, Wen; Wang, Han; Zhou, Gao; Chen, Xing; Yang, Hui; Hong, Lingzi; Zhao, Junjie; Qin, Luke; Fukuda, Koichi; Flotho, Annette; Gao, Ji; Dongre, Ashok; Carman, Julie A; Kang, Zizhen; Su, Bing; Kern, Timothy S; Smith, Jonathan D; Hamilton, Thomas A; Melchior, Frauke; Fox, Paul L

    2017-01-01

    Expression of inflammatory genes is determined in part by post-transcriptional regulation of mRNA metabolism but how stimulus- and transcript-dependent nuclear export influence is poorly understood. Here, we report a novel pathway in which LPS/TLR4 engagement promotes nuclear localization of IRAK2 to facilitate nuclear export of a specific subset of inflammation-related mRNAs for translation in murine macrophages. IRAK2 kinase activity is required for LPS-induced RanBP2-mediated IRAK2 sumoylation and subsequent nuclear translocation. Array analysis showed that an SRSF1-binding motif is enriched in mRNAs dependent on IRAK2 for nuclear export. Nuclear IRAK2 phosphorylates SRSF1 to reduce its binding to target mRNAs, which promotes the RNA binding of the nuclear export adaptor ALYREF and nuclear export receptor Nxf1 loading for the export of the mRNAs. In summary, LPS activates a nuclear function of IRAK2 that facilitates the assembly of nuclear export machinery to export selected inflammatory mRNAs to the cytoplasm for translation. PMID:28990926

  8. IRAK2 directs stimulus-dependent nuclear export of inflammatory mRNAs.

    PubMed

    Zhou, Hao; Bulek, Katarzyna; Li, Xiao; Herjan, Tomasz; Yu, Minjia; Qian, Wen; Wang, Han; Zhou, Gao; Chen, Xing; Yang, Hui; Hong, Lingzi; Zhao, Junjie; Qin, Luke; Fukuda, Koichi; Flotho, Annette; Gao, Ji; Dongre, Ashok; Carman, Julie A; Kang, Zizhen; Su, Bing; Kern, Timothy S; Smith, Jonathan D; Hamilton, Thomas A; Melchior, Frauke; Fox, Paul L; Li, Xiaoxia

    2017-10-09

    Expression of inflammatory genes is determined in part by post-transcriptional regulation of mRNA metabolism but how stimulus- and transcript-dependent nuclear export influence is poorly understood. Here, we report a novel pathway in which LPS/TLR4 engagement promotes nuclear localization of IRAK2 to facilitate nuclear export of a specific subset of inflammation-related mRNAs for translation in murine macrophages. IRAK2 kinase activity is required for LPS-induced RanBP2-mediated IRAK2 sumoylation and subsequent nuclear translocation. Array analysis showed that an SRSF1-binding motif is enriched in mRNAs dependent on IRAK2 for nuclear export. Nuclear IRAK2 phosphorylates SRSF1 to reduce its binding to target mRNAs, which promotes the RNA binding of the nuclear export adaptor ALYREF and nuclear export receptor Nxf1 loading for the export of the mRNAs. In summary, LPS activates a nuclear function of IRAK2 that facilitates the assembly of nuclear export machinery to export selected inflammatory mRNAs to the cytoplasm for translation.

  9. Chemical Manufacturers, Importers, and Exporters: Frequent Questions

    EPA Pesticide Factsheets

    Chemical manufacturers, importers, and exporters are required to operate within a system of allowances. This information will help those in the chemical industry understand their role in the phaseout of HCFCs in the United States.

  10. Cex1p facilitates Rna1p-mediated dissociation of the Los1p-tRNA-Gsp1p-GTP export complex.

    PubMed

    McGuire, Andrew T; Mangroo, Dev

    2012-02-01

    Nuclear tRNA export plays an essential role in key cellular processes such as regulation of protein synthesis, cell cycle progression, response to nutrient availability and DNA damage and development. Like other nuclear export processes, assembly of the nuclear tRNA export complex in the nucleus is dependent on Ran-GTP/Gsp1p-GTP, and dissociation of the export receptor-tRNA-Ran-GTP/Gsp1p-GTP complex in the cytoplasm requires RanBP1/Yrb1p and RanGAP/Rna1p to activate the GTPase activity of Ran-GTP/Gsp1p-GTP. The Saccharomyces cerevisiae Cex1p and Human Scyl1 have also been proposed to participate in unloading of the tRNA export receptors at the cytoplasmic face of the nuclear pore complex (NPC). Here, we provide evidence suggesting that Cex1p is required for activation of the GTPase activity of Gsp1p and dissociation of the receptor-tRNA-Gsp1p export complex in S. cerevisiae. The data suggest that Cex1p recruits Rna1p from the cytoplasm to the NPC and facilitates Rna1p activation of the GTPase activity of Gsp1p by enabling Rna1p to gain access to Gsp1p-GTP bound to the export receptor tRNA complex. It is possible that this tRNA unloading mechanism is conserved in evolutionarily diverse organisms and that other Gsp1p-GTP-dependent export processes use a pathway-specific component to recruit Rna1p to the NPC. © 2011 John Wiley & Sons A/S.

  11. 31 CFR 545.406 - Exportation of services; performance of service contracts; legal services.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... financial services. ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Exportation of services; performance of service contracts; legal services. 545.406 Section 545.406 Money and Finance: Treasury Regulations...

  12. Natural gas imports and exports, first quarter report 2000

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    The Office of Natural Gas and Petroleum Import and Export Activities prepares quarterly reports showing natural gas import and export activity. Companies are required to file quarterly reports. Attachments show the percentage of takes to maximum firm contract levels and the weighted average per unit price for each of the long-term importers during the 5 most recent reporting quarters, volumes and prices of gas purchased by long-term importers and exporters during the past 12 months, volume and price data for gas imported on a short-term or spot market basis, and the gas exported on a short-term or spot market basismore » to Canada and Mexico.« less

  13. Natural gas imports and exports, fourth quarter report 1999

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    The Office of Natural Gas and Petroleum Import and Export Activities prepares quarterly reports showing natural gas import and export activity. Companies are required to file quarterly reports. Attachments show the percentage of takes to maximum firm contract levels and the weighted average per unit price for each of the long-term importers during the five most recent quarters, volumes and prices of gas purchased by long-term importers and exporters during the past 12 months, volume and price data for gas imported on a short-term or spot market basis, and the gas exported on a short-term or spot market basis tomore » Canada and Mexico.« less

  14. Nuclear Export Inhibition Enhances HLH-30/TFEB Activity, Autophagy, and Lifespan.

    PubMed

    Silvestrini, Melissa J; Johnson, Joseph R; Kumar, Anita V; Thakurta, Tara G; Blais, Karine; Neill, Zachary A; Marion, Sarah W; St Amand, Victoria; Reenan, Robert A; Lapierre, Louis R

    2018-05-15

    Transcriptional modulation of the process of autophagy involves the transcription factor HLH-30/TFEB. In order to systematically determine the regulatory network of HLH-30/TFEB, we performed a genome-wide RNAi screen in C. elegans and found that silencing the nuclear export protein XPO-1/XPO1 enhances autophagy by significantly enriching HLH-30 in the nucleus, which is accompanied by proteostatic benefits and improved longevity. Lifespan extension via xpo-1 silencing requires HLH-30 and autophagy, overlapping mechanistically with several established longevity models. Selective XPO1 inhibitors recapitulated the effect on autophagy and lifespan observed by silencing xpo-1 and protected ALS-afflicted flies from neurodegeneration. XPO1 inhibition in HeLa cells enhanced TFEB nuclear localization, autophagy, and lysosome biogenesis without affecting mTOR activity, revealing a conserved regulatory mechanism for HLH-30/TFEB. Altogether, our study demonstrates that altering the nuclear export of HLH-30/TFEB can regulate autophagy and establishes the rationale of targeting XPO1 to stimulate autophagy in order to prevent neurodegeneration. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

  15. An application of seasonal ARIMA models on group commodities to forecast Philippine merchandise exports performance

    NASA Astrophysics Data System (ADS)

    Natividad, Gina May R.; Cawiding, Olive R.; Addawe, Rizavel C.

    2017-11-01

    The increase in the merchandise exports of the country offers information about the Philippines' trading role within the global economy. Merchandise exports statistics are used to monitor the country's overall production that is consumed overseas. This paper investigates the comparison between two models obtained by a) clustering the commodity groups into two based on its proportional contribution to the total exports, and b) treating only the total exports. Different seasonal autoregressive integrated moving average (SARIMA) models were then developed for the clustered commodities and for the total exports based on the monthly merchandise exports of the Philippines from 2011 to 2016. The data set used in this study was retrieved from the Philippine Statistics Authority (PSA) which is the central statistical authority in the country responsible for primary data collection. A test for significance of the difference between means at 0.05 level of significance was then performed on the forecasts produced. The result indicates that there is a significant difference between the mean of the forecasts of the two models. Moreover, upon a comparison of the root mean square error (RMSE) and mean absolute error (MAE) of the models, it was found that the models used for the clustered groups outperform the model for the total exports.

  16. Information for Exporters of Resource Conservation and Recovery Act (RCRA) Hazardous Waste

    EPA Pesticide Factsheets

    Information for exporters of hazardous waste to OECD countries for recycling who are subject to the hazardous waste generator and importer requirements described in 40 CFR Part 262 Subpart H, under RCRA

  17. Cycling of the signaling protein phospholipase D through cilia requires the BBSome only for the export phase

    PubMed Central

    Brown, Jason M.; Sampaio, Julio L.; Craft, Julie M.; Shevchenko, Andrej; Evans, James E.; Witman, George B.

    2013-01-01

    The BBSome is a complex of seven proteins, including BBS4, that is cycled through cilia by intraflagellar transport (IFT). Previous work has shown that the membrane-associated signaling protein phospholipase D (PLD) accumulates abnormally in cilia of Chlamydomonas reinhardtii bbs mutants. Here we show that PLD is a component of wild-type cilia but is enriched ∼150-fold in bbs4 cilia; this accumulation occurs progressively over time and results in altered ciliary lipid composition. When wild-type BBSomes were introduced into bbs cells, PLD was rapidly removed from the mutant cilia, indicating the presence of an efficient BBSome-dependent mechanism for exporting ciliary PLD. This export requires retrograde IFT. Importantly, entry of PLD into cilia is BBSome and IFT independent. Therefore, the BBSome is required only for the export phase of a process that continuously cycles PLD through cilia. Another protein, carbonic anhydrase 6, is initially imported normally into bbs4 cilia but lost with time, suggesting that its loss is a secondary effect of BBSome deficiency. PMID:23589493

  18. 15 CFR 714.2 - Annual reporting requirements for exports and imports in excess of 30 metric tons of Schedule 3...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... plant site is declared for production of a Schedule 3 chemical (and has completed questions 3-3.1 and 3.... (ii) If your plant site is declared for production of a Schedule 3 chemical and you exported or... exports and imports in excess of 30 metric tons of Schedule 3 chemicals. 714.2 Section 714.2 Commerce and...

  19. 15 CFR 714.2 - Annual reporting requirements for exports and imports in excess of 30 metric tons of Schedule 3...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... plant site is declared for production of a Schedule 3 chemical (and has completed questions 3-3.1 and 3.... (ii) If your plant site is declared for production of a Schedule 3 chemical and you exported or... exports and imports in excess of 30 metric tons of Schedule 3 chemicals. 714.2 Section 714.2 Commerce and...

  20. 15 CFR 714.2 - Annual reporting requirements for exports and imports in excess of 30 metric tons of Schedule 3...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... plant site is declared for production of a Schedule 3 chemical (and has completed questions 3-3.1 and 3.... (ii) If your plant site is declared for production of a Schedule 3 chemical and you exported or... exports and imports in excess of 30 metric tons of Schedule 3 chemicals. 714.2 Section 714.2 Commerce and...

  1. 15 CFR 744.9 - Restrictions on certain exports and reexports of cameras controlled by ECCN 6A003.b.4.b.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... reexports of cameras controlled by ECCN 6A003.b.4.b. 744.9 Section 744.9 Commerce and Foreign Trade... on certain exports and reexports of cameras controlled by ECCN 6A003.b.4.b. (a) General prohibitions... license is required to export or reexport to any destination other than Canada cameras described in ECCN...

  2. 15 CFR 744.9 - Restrictions on certain exports and reexports of cameras controlled by ECCN 6A003.b.4.b.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... reexports of cameras controlled by ECCN 6A003.b.4.b. 744.9 Section 744.9 Commerce and Foreign Trade... on certain exports and reexports of cameras controlled by ECCN 6A003.b.4.b. (a) General prohibitions... license is required to export or reexport to any destination other than Canada cameras described in ECCN...

  3. 15 CFR 744.9 - Restrictions on certain exports and reexports of cameras controlled by ECCN 6A003.b.4.b.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... reexports of cameras controlled by ECCN 6A003.b.4.b. 744.9 Section 744.9 Commerce and Foreign Trade... on certain exports and reexports of cameras controlled by ECCN 6A003.b.4.b. (a) General prohibitions... license is required to export or reexport to any destination other than Canada cameras described in ECCN...

  4. Molecule database framework: a framework for creating database applications with chemical structure search capability

    PubMed Central

    2013-01-01

    Background Research in organic chemistry generates samples of novel chemicals together with their properties and other related data. The involved scientists must be able to store this data and search it by chemical structure. There are commercial solutions for common needs like chemical registration systems or electronic lab notebooks. However for specific requirements of in-house databases and processes no such solutions exist. Another issue is that commercial solutions have the risk of vendor lock-in and may require an expensive license of a proprietary relational database management system. To speed up and simplify the development for applications that require chemical structure search capabilities, I have developed Molecule Database Framework. The framework abstracts the storing and searching of chemical structures into method calls. Therefore software developers do not require extensive knowledge about chemistry and the underlying database cartridge. This decreases application development time. Results Molecule Database Framework is written in Java and I created it by integrating existing free and open-source tools and frameworks. The core functionality includes: • Support for multi-component compounds (mixtures) • Import and export of SD-files • Optional security (authorization) For chemical structure searching Molecule Database Framework leverages the capabilities of the Bingo Cartridge for PostgreSQL and provides type-safe searching, caching, transactions and optional method level security. Molecule Database Framework supports multi-component chemical compounds (mixtures). Furthermore the design of entity classes and the reasoning behind it are explained. By means of a simple web application I describe how the framework could be used. I then benchmarked this example application to create some basic performance expectations for chemical structure searches and import and export of SD-files. Conclusions By using a simple web application it was shown that Molecule Database Framework successfully abstracts chemical structure searches and SD-File import and export to simple method calls. The framework offers good search performance on a standard laptop without any database tuning. This is also due to the fact that chemical structure searches are paged and cached. Molecule Database Framework is available for download on the projects web page on bitbucket: https://bitbucket.org/kienerj/moleculedatabaseframework. PMID:24325762

  5. Molecule database framework: a framework for creating database applications with chemical structure search capability.

    PubMed

    Kiener, Joos

    2013-12-11

    Research in organic chemistry generates samples of novel chemicals together with their properties and other related data. The involved scientists must be able to store this data and search it by chemical structure. There are commercial solutions for common needs like chemical registration systems or electronic lab notebooks. However for specific requirements of in-house databases and processes no such solutions exist. Another issue is that commercial solutions have the risk of vendor lock-in and may require an expensive license of a proprietary relational database management system. To speed up and simplify the development for applications that require chemical structure search capabilities, I have developed Molecule Database Framework. The framework abstracts the storing and searching of chemical structures into method calls. Therefore software developers do not require extensive knowledge about chemistry and the underlying database cartridge. This decreases application development time. Molecule Database Framework is written in Java and I created it by integrating existing free and open-source tools and frameworks. The core functionality includes:•Support for multi-component compounds (mixtures)•Import and export of SD-files•Optional security (authorization)For chemical structure searching Molecule Database Framework leverages the capabilities of the Bingo Cartridge for PostgreSQL and provides type-safe searching, caching, transactions and optional method level security. Molecule Database Framework supports multi-component chemical compounds (mixtures).Furthermore the design of entity classes and the reasoning behind it are explained. By means of a simple web application I describe how the framework could be used. I then benchmarked this example application to create some basic performance expectations for chemical structure searches and import and export of SD-files. By using a simple web application it was shown that Molecule Database Framework successfully abstracts chemical structure searches and SD-File import and export to simple method calls. The framework offers good search performance on a standard laptop without any database tuning. This is also due to the fact that chemical structure searches are paged and cached. Molecule Database Framework is available for download on the projects web page on bitbucket: https://bitbucket.org/kienerj/moleculedatabaseframework.

  6. 7 CFR 17.4 - Agents of the participant or importer.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Administrator, Export Credits, FAS, along with a copy of the proposed agreement between the participant or..., Export Credits, FAS, has provided a written statement that the nomination is accepted in accordance with... perform functions of a shipping agent, shall furnish to the Deputy Administrator, Export Credits, FAS, the...

  7. 7 CFR 17.4 - Agents of the participant or importer.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Administrator, Export Credits, FAS, along with a copy of the proposed agreement between the participant or..., Export Credits, FAS, has provided a written statement that the nomination is accepted in accordance with... perform functions of a shipping agent, shall furnish to the Deputy Administrator, Export Credits, FAS, the...

  8. 7 CFR 17.4 - Agents of the participant or importer.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Administrator, Export Credits, FAS, along with a copy of the proposed agreement between the participant or..., Export Credits, FAS, has provided a written statement that the nomination is accepted in accordance with... perform functions of a shipping agent, shall furnish to the Deputy Administrator, Export Credits, FAS, the...

  9. 7 CFR 17.4 - Agents of the participant or importer.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Administrator, Export Credits, FAS, along with a copy of the proposed agreement between the participant or..., Export Credits, FAS, has provided a written statement that the nomination is accepted in accordance with... perform functions of a shipping agent, shall furnish to the Deputy Administrator, Export Credits, FAS, the...

  10. 7 CFR 17.4 - Agents of the participant or importer.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Administrator, Export Credits, FAS, along with a copy of the proposed agreement between the participant or..., Export Credits, FAS, has provided a written statement that the nomination is accepted in accordance with... perform functions of a shipping agent, shall furnish to the Deputy Administrator, Export Credits, FAS, the...

  11. 40 CFR 80.1464 - What are the attest engagement requirements under the RFS program?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... any of these documents refer to the exported fuel as advanced biofuel or cellulosic biofuel; and report as a finding whether or not the exporter calculated an advanced biofuel or cellulosic biofuel RVO...

  12. 40 CFR 80.1464 - What are the attest engagement requirements under the RFS program?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... any of these documents refer to the exported fuel as advanced biofuel or cellulosic biofuel; and report as a finding whether or not the exporter calculated an advanced biofuel or cellulosic biofuel RVO...

  13. 40 CFR 80.1464 - What are the attest engagement requirements under the RFS program?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... any of these documents refer to the exported fuel as advanced biofuel or cellulosic biofuel; and report as a finding whether or not the exporter calculated an advanced biofuel or cellulosic biofuel RVO...

  14. 40 CFR 80.1464 - What are the attest engagement requirements under the RFS program?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... any of these documents refer to the exported fuel as advanced biofuel or cellulosic biofuel; and report as a finding whether or not the exporter calculated an advanced biofuel or cellulosic biofuel RVO...

  15. 40 CFR 80.1464 - What are the attest engagement requirements under the RFS program?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... any of these documents refer to the exported fuel as advanced biofuel or cellulosic biofuel; and report as a finding whether or not the exporter calculated an advanced biofuel or cellulosic biofuel RVO...

  16. Petrobactin Is Exported from Bacillus anthracis by the RND-Type Exporter ApeX

    PubMed Central

    Hagan, A. K.; Berger, D.

    2017-01-01

    ABSTRACT Bacillus anthracis—a Gram-positive, spore-forming bacterium—causes anthrax, a highly lethal disease with high bacteremia titers. Such rapid growth requires ample access to nutrients, including iron. However, access to this critical metal is heavily restricted in mammals, which requires B. anthracis to employ petrobactin, an iron-scavenging small molecule known as a siderophore. Petrobactin biosynthesis is mediated by asb gene products, and import of the iron-bound (holo)-siderophore into the bacterium has been well studied. In contrast, little is known about the mechanism of petrobactin export following its production in B. anthracis cells. Using a combination of bioinformatics data, gene deletions, and laser ablation electrospray ionization mass spectrometry (LAESI-MS), we identified a resistance-nodulation-cell division (RND)-type transporter, termed ApeX, as a putative petrobactin exporter. Deletion of apeX abrogated export of intact petrobactin, which accumulated inside the cell. However, growth of ΔapeX mutants in iron-depleted medium was not affected, and virulence in mice was not attenuated. Instead, petrobactin components were determined to be exported through a different protein, which enables iron transport sufficient for growth, albeit with a slightly lower affinity for iron. This is the first report to identify a functional siderophore exporter in B. anthracis and the in vivo functionality of siderophore components. Moreover, this is the first application of LAESI-MS to sample a virulence factor/metabolite directly from bacterial culture media and cell pellets of a human pathogen. PMID:28900020

  17. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Report of Requests for Restrictive Trade Practice or Boycott—Single or Multiple Transactions part 760 and § 762.2(b). 0694-0013 Computers and Related Equipment EAR Supplement 2 to Part 748 part 774. 0694-0016... §§ 762.2(b) and 764.5. 0694-0073 Export Controls of High Performance Computers Supplement No. 2 to part...

  18. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Report of Requests for Restrictive Trade Practice or Boycott—Single or Multiple Transactions part 760 and § 762.2(b). 0694-0013 Computers and Related Equipment EAR Supplement 2 to Part 748 part 774. 0694-0016... §§ 762.2(b) and 764.5. 0694-0073 Export Controls of High Performance Computers Supplement No. 2 to part...

  19. 15 CFR Supplement No. 1 to Part 730 - Information Collection Requirements Under the Paperwork Reduction Act: OMB Control Numbers

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Report of Requests for Restrictive Trade Practice or Boycott—Single or Multiple Transactions part 760 and § 762.2(b). 0694-0013 Computers and Related Equipment EAR Supplement 2 to Part 748 part 774. 0694-0016... §§ 762.2(b) and 764.5. 0694-0073 Export Controls of High Performance Computers Supplement No. 2 to part...

  20. Brillouin distributed temperature sensing system for monitoring of submarine export cables of off-shore wind farms

    NASA Astrophysics Data System (ADS)

    Marx, Benjamin; Rath, Alexander; Kolm, Frederick; Schröder, Andreas; Buntebarth, Christian; Dreß, Albrecht; Hill, Wieland

    2016-05-01

    For high-voltage cables, the maximum temperature of the insulation must never be exceeded at any location and at any load condition. The local temperatures depend not only on the cable design and load history, but also on the local thermal environment of the cable. Therefore, distributed temperature monitoring of high-voltage cables is essential to ensure the integrity of the cable at high load. Especially, the load of the export cables of wind farms varies strongly in dependence on weather conditions. In this field study, we demonstrate the measurement performance of a new, robust Brillouin distributed temperature sensing system (Brillouin-DTS). The system is based on spontaneous Brillouin scattering and does not require a fibre loop. This is essential for long submarine high-voltage cables, where normally no loop can be formed in the seabed. It is completely passively cooled and does not contain any moving or wearing parts. The instrument is dedicated for use in industrial and other rough environments. With a measuring time below 10 min, the temperature resolution is better than 1 °C for distances up to 50 km. In the field study, the submarine export cable of an off-shore wind farm has been monitored. The temperature profile of the export cable shows several hot spots, mostly located at cable joints, and also several cold spots.

  1. The C-terminal portion of the cleaved HT motif is necessary and sufficient to mediate export of proteins from the malaria parasite into its host cell

    PubMed Central

    Tarr, Sarah J; Cryar, Adam; Thalassinos, Konstantinos; Haldar, Kasturi; Osborne, Andrew R

    2013-01-01

    The malaria parasite exports proteins across its plasma membrane and a surrounding parasitophorous vacuole membrane, into its host erythrocyte. Most exported proteins contain a Host Targeting motif (HT motif) that targets them for export. In the parasite secretory pathway, the HT motif is cleaved by the protease plasmepsin V, but the role of the newly generated N-terminal sequence in protein export is unclear. Using a model protein that is cleaved by an exogenous viral protease, we show that the new N-terminal sequence, normally generated by plasmepsin V cleavage, is sufficient to target a protein for export, and that cleavage by plasmepsin V is not coupled directly to the transfer of a protein to the next component in the export pathway. Mutation of the fourth and fifth positions of the HT motif, as well as amino acids further downstream, block or affect the efficiency of protein export indicating that this region is necessary for efficient export. We also show that the fifth position of the HT motif is important for plasmepsin V cleavage. Our results indicate that plasmepsin V cleavage is required to generate a new N-terminal sequence that is necessary and sufficient to mediate protein export by the malaria parasite. PMID:23279267

  2. Estimating the number of trees and forest area necessary to supply internationally traded volumes of big-leaf mahogany (Swietenia macrophylla) in Amazonia

    Treesearch

    J. Grogan; M. Schulze

    2008-01-01

    requires producer nations to certify that exported supplies were obtained in a manner non-detrimental to the species’ survival in its role in the ecosystem. Non-detriment findings based on annual export quotas should verify that current harvest rates are sustainable with respect to total commercial stocks. In order to assess this impact, a method for converting export...

  3. 40 CFR 262.50 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE Exports of Hazardous Waste § 262.50 Applicability. This subpart establishes requirements applicable to exports of hazardous waste. Except to the extent § 262.58 provides...

  4. 50 CFR 300.185 - Documentation, reporting and recordkeeping requirements for consignment documents and re-export...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... issuing country must be written legibly and indelibly on the outside of the package. (ix) Customs forms... permit holder must email, fax, or mail a copy of the completed consignment document and re-export...

  5. Measuring the gradualist approach to internationalization: Empirical evidence from the wine sector

    PubMed Central

    2018-01-01

    The objective of this paper is to fill a gap in the literature on internationalization, in relation to the absence of objective and measurable performance indicators for the process of how firms sequentially enter external markets. To that end, this research develops a quantitative tool for use as a performance indicator of gradualness for firms entering external markets at a sectoral level. The performance indicator is based on firms’ export volumes, number of years operating in the export market, geographic areas targeted for export and when exports began to each area. The indicator is tested empirically in the wine sector. The main contribution of this study is the creation of a reliable international priority index, which can serve more widely as a valuable tool because of its potential use in other industry sectors and geographic areas, and which would allow the analysis of how geographically differentiated internationalization strategies develop. PMID:29727461

  6. Measuring the gradualist approach to internationalization: Empirical evidence from the wine sector.

    PubMed

    Clavel San Emeterio, Mónica; Fernández-Ortiz, Rubén; Arteaga-Ortiz, Jesús; Dorta-González, Pablo

    2018-01-01

    The objective of this paper is to fill a gap in the literature on internationalization, in relation to the absence of objective and measurable performance indicators for the process of how firms sequentially enter external markets. To that end, this research develops a quantitative tool for use as a performance indicator of gradualness for firms entering external markets at a sectoral level. The performance indicator is based on firms' export volumes, number of years operating in the export market, geographic areas targeted for export and when exports began to each area. The indicator is tested empirically in the wine sector. The main contribution of this study is the creation of a reliable international priority index, which can serve more widely as a valuable tool because of its potential use in other industry sectors and geographic areas, and which would allow the analysis of how geographically differentiated internationalization strategies develop.

  7. Assessing mRNA nuclear export in mammalian cells by microinjection.

    PubMed

    Lee, Eliza S; Palazzo, Alexander F

    2017-08-15

    The nuclear export of mRNAs is an important yet little understood part of eukaryotic gene expression. One of the easiest methods for monitoring mRNA export in mammalian tissue culture cells is through the microinjection of DNA plasmids into the nucleus and monitoring the distribution of the transcribed product over time. Here we describe how to setup a microscope equipped with a micromanipulator used in cell microinjections, and we explain how to perform a nuclear mRNA export assay and obtain the nuclear export rate for any given mRNA. Copyright © 2017 Elsevier Inc. All rights reserved.

  8. Inspection of the Department`s export licensing process for dual-use and munitions commodities

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1993-08-10

    The purpose of our inspection was to review the Department of Energy`s (Energy) export licensing process for dual-use and military (munitions) commodities subject to nuclear nonproliferation controls. Specifically, we reviewed Energy`s authorities, procedures, and policies pertaining to the export licensing process and examined procedures for safeguarding data transmitted between Energy and other agencies involved in the export licensing process. We also reviewed Energy`s role as a member of the Subgroup on Nuclear Export Coordination. Our review of the sample of 60 export cases did not find evidence to lead us to believe that Energy`s recommendations for these cases were inappropriatemore » or incorrect. We identified, however, problems regarding management systems associated with the export license review process. We found that without documentation supporting export licensing decisions by the Export Control Operations Division (ECOD), we could not determine whether ECOD analysts considered all required criteria in their review of export cases referred to Energy. For example, we found that the ECOD did not retain records documenting the bases for its advice, recommendations, or decisions regarding its reviews of export license cases or revisions to lists of controlled commodities and, therefore, was not in compliance with certain provisions of the Export Administration Act, as amended, and Energy records management directives. Additionally, we found that the degree of compliance by Energy with the export licensing review criteria contained in the Export Administration Regulations and the Nuclear Non-Proliferation Act of 1978 could not be determined because ECOD did not retain records documenting the bases for its advice and recommendations on export cases.« less

  9. 78 FR 18560 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... 1494.301), (2) performance security (section 1494.401), (3) export sales information in connection with...) whether the collection of information is necessary for the proper performance of the functions of the... the bonus (section 1494.701) In addition, each exporter must maintain accurate records showing sales...

  10. 7 CFR 1493.290 - Proof of entry.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Facility Guarantee Program (FGP... certified or other translation acceptable to CCC. Records acceptable to meet this requirement include: (1... documentation deemed acceptable by CCC: (i) Showing that the goods entered the emerging market; (ii) Identifying...

  11. 7 CFR 1493.290 - Proof of entry.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Facility Guarantee Program (FGP... certified or other translation acceptable to CCC. Records acceptable to meet this requirement include: (1... documentation deemed acceptable by CCC: (i) Showing that the goods entered the emerging market; (ii) Identifying...

  12. 7 CFR 1493.290 - Proof of entry.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Facility Guarantee Program (FGP... certified or other translation acceptable to CCC. Records acceptable to meet this requirement include: (1... documentation deemed acceptable by CCC: (i) Showing that the goods entered the emerging market; (ii) Identifying...

  13. Translations on Eastern Europe, Economic and Industrial Affairs, Number 1710

    DTIC Science & Technology

    1977-12-30

    Number of Stores To Be Increased (Gerhard Briksa; PRESSE-INFORMATIONEN, 11 Nov 77) 1 Greater Efforts Needed for Exports (Karl-Heinz Arnold...EAST GERMANY GREATER EFFORTS NEEDED FOR EXPORTS East Berlin BERLINER ZEITUNG in German 12-13 Nov 77 p 9 [Commentary by Dr Karl-Heinz Arnold...Text] This fourth quarter once again requires special efforts in order to reach the export goals for the year as well as possible and not to begin at

  14. DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    The Office of Natural Gas and Petroleum Import and Export Activities prepares quarterly reports showing natural gas import and export activity. Companies are required to file quarterly reports. Attachments show the percentage of takes to maximum firm contract levels and the weighted average per unit price for each of the long-term importers during the 5 most recent quarters, volumes and prices of gas purchased by long-term importers and exporters during the past 12 months, volume and price data for gas imported on a short-term or spot market basis, and the gas exported on a short-term or spot market basis tomore » Canada and Mexico.« less

  15. Antecedents to the Export Market Orientation of Hungarian Higher Education Institutions, and Their Export Performance Consequences

    ERIC Educational Resources Information Center

    Nagy, Gabor; Beracs, Jozsef

    2012-01-01

    Research into the international competitiveness of the higher education sector has shown that the approaches which management literature has introduced into the business sphere during the last half-century are being more and more frequently applied by successful universities as well. The authors extend the theory of export market orientation to…

  16. 78 FR 69438 - AGOA: Trade and Investment Performance Overview; AGOA: Economic Effects of Providing Duty-Free...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-19

    ... To Promote Regional Integration and Increase Exports to the United States; EU-South Africa FTA... Integration and Increase Exports to the United States; and investigation No. 332-546, EU-South Africa FTA... integration into regional or global supply chains, and export potential to the United States under AGOA, as...

  17. 7 CFR 1493.480 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.480 Certification... payment guarantee, CCC will have the right, notwithstanding any other rights provided under this subpart...

  18. 7 CFR 1493.310 - Payment for loss.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Facility Guarantee Program (FGP) Operations § 1493.310 Payment for loss. (a) Determination of CCC's liability. Upon receipt in good order of the information and documents required under § 1493.300, CCC will determine whether or...

  19. 7 CFR 1493.480 - Certification requirements for the evidence of export.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.480 Certification... payment guarantee, CCC will have the right, notwithstanding any other rights provided under this subpart...

  20. 7 CFR 1493.310 - Payment for loss.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Facility Guarantee Program (FGP) Operations § 1493.310 Payment for loss. (a) Determination of CCC's liability. Upon receipt in good order of the information and documents required under § 1493.300, CCC will determine whether or...

  1. 7 CFR 1493.310 - Payment for loss.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Facility Guarantee Program (FGP) Operations § 1493.310 Payment for loss. (a) Determination of CCC's liability. Upon receipt in good order of the information and documents required under § 1493.300, CCC will determine whether or...

  2. Adjusting export tax rebates to reduce the environmental impacts of trade: Lessons from China.

    PubMed

    Song, Peng; Mao, Xianqiang; Corsetti, Gabriel

    2015-09-15

    Export tax rebates are an important policy instrument for stimulating exports, which many developing countries make use of. However, excessive export tax rebates and inappropriate structural arrangements can lead to over-production in highly polluting industries and cause the environment to deteriorate. This paper, taking China as the study case, tests and verifies the statistical significance of the causal relationship between export tax rebates and pollution emissions. With a computable general equilibrium modeling, the current study further analyzes the effectiveness of export tax rebate adjustments aimed at alleviating environmental pressure for different time periods. It is found that before 2003, export tax rebates primarily promoted exports and boosted foreign exchange reserves, and highly polluting sectors enjoyed above-average export tax rebates, which led to increased pollution emissions. Between 2003 and 2010, the export tax rebate system was reformed to reduce support for the highly polluting export sectors, which led to decreases in emissions. Canceling export tax rebates for highly polluting sectors is shown to be the most favorable policy choice for improving the environmental performance of China's international trade. This study can serve as reference for other developing countries which similarly rely on export tax rebates, so that they can adjust their policies so as to combine economic growth with pollution control. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Pertussis toxin export genes are regulated by the ptx promoter and may be required for efficient translation of ptx mRNA in Bordetella pertussis.

    PubMed Central

    Baker, S M; Masi, A; Liu, D F; Novitsky, B K; Deich, R A

    1995-01-01

    The gene products from an 8-kb region adjacent to the 3' end of the ptx operon are required by Bordetella pertussis for the export of pertussis holotoxin. At least one of these gene products (PtlC) is specifically required for the export of assembled holotoxin from the periplasmic space. ptlC mutants exhibit a 20-fold reduction in the amount of holotoxin present in the culture supernatant but have no effect upon the assembly or steady-state level of holotoxin present in the periplasmic space. Impaired export of holotoxin from the ptlC strain blocks expression of toxin at a posttranscriptional level, and wild-type levels of ptx mRNA are detected in the mutant strain. The transcription of ptl is subject to modulation by MgSO4 in the same manner as ptx is; however, in B. pertussis strains containing an E. coli tac promoter in place of the native ptx promoter, wild-type levels of ptx mRNA are present and holotoxin is synthesized and exported even in the presence of MgSO4. Promoter mapping of the region extending from the ptxS3 coding region to the ptlC coding region failed to detect the ptl transcription initiation site. Additional RNase protection experiments with ptx promoter deletion and substitution strains indicate that the ptl operon is transcribed from the ptx promoter as part of a > 11-kb mRNA. PMID:7558300

  4. RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Huang, Feng; Zhang, Junsong; Zhang, Yijun

    Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various steps. However, the endogenous MOV10 was required in certain step(s) of HIV-1 replication. In this report, we found that MOV10 potently enhances the nuclear export of viral mRNAs and subsequently increases the expression of Gag protein and other late products through affecting the Rev/RRE axis. The co-immunoprecipitation analysis indicated that MOV10 interacts with Rev in an RNA-independent manner. The DEAG-boxmore » of MOV10 was required for the enhancement of Rev/RRE-dependent nuclear export and the DEAG-box mutant showed a dominant-negative activity. Our data propose that HIV-1 utilizes the anti-viral factor MOV10 to function as a co-factor of Rev and demonstrate the complicated effects of MOV10 on HIV-1 life cycle. - Highlights: • MOV10 can function as a co-factor of HIV-1 Rev. • MOV10 facilitates Rev/RRE-dependent transport of viral mRNAs. • MOV10 interacts with Rev in an RNA-independent manner. • The DEAG-box of MOV10 is required for the enhancement of Rev/RRE-dependent export.« less

  5. Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export

    PubMed Central

    Zhang, Liang; Das, Priyabrata; Schmolke, Mirco; Manicassamy, Balaji; Wang, Yaming; Deng, Xiaoyi; Cai, Ling; Tu, Benjamin P.; Forst, Christian V.; Roth, Michael G.; Levy, David E.; García-Sastre, Adolfo; de Brabander, Jef; Phillips, Margaret A.

    2012-01-01

    The NS1 protein of influenza virus is a major virulence factor essential for virus replication, as it redirects the host cell to promote viral protein expression. NS1 inhibits cellular messenger ribonucleic acid (mRNA) processing and export, down-regulating host gene expression and enhancing viral gene expression. We report in this paper the identification of a nontoxic quinoline carboxylic acid that reverts the inhibition of mRNA nuclear export by NS1, in the absence or presence of the virus. This quinoline carboxylic acid directly inhibited dihydroorotate dehydrogenase (DHODH), a host enzyme required for de novo pyrimidine biosynthesis, and partially reduced pyrimidine levels. This effect induced NXF1 expression, which promoted mRNA nuclear export in the presence of NS1. The release of NS1-mediated mRNA export block by DHODH inhibition also occurred in the presence of vesicular stomatitis virus M (matrix) protein, another viral inhibitor of mRNA export. This reversal of mRNA export block allowed expression of antiviral factors. Thus, pyrimidines play a necessary role in the inhibition of mRNA nuclear export by virulence factors. PMID:22312003

  6. Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2

    PubMed Central

    Occhipinti, Laura; Chang, Yiming; Altvater, Martin; Menet, Anna M.; Kemmler, Stefan; Panse, Vikram G.

    2013-01-01

    Multiple export receptors passage bound pre-ribosomes through nuclear pore complexes (NPCs) by transiently interacting with the Phe-Gly (FG) meshwork of their transport channels. Here, we reveal how the non-FG interacting yeast mRNA export factor Gly-Leu-FG lethal 2 (Gle2) functions in the export of the large pre-ribosomal subunit (pre-60S). Structure-guided studies uncovered conserved platforms used by Gle2 to export pre-60S: an uncharacterized basic patch required to bind pre-60S, and a second surface that makes non-FG contacts with the nucleoporin Nup116. A basic patch mutant of Gle2 is able to function in mRNA export, but not pre-60S export. Thus, Gle2 provides a distinct interaction platform to transport pre-60S to the cytoplasm. Notably, Gle2’s interaction platforms become crucial for pre-60S export when FG-interacting receptors are either not recruited to pre-60S or are impaired. We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel. PMID:23907389

  7. Hierarchical protein export mechanism of the bacterial flagellar type III protein export apparatus.

    PubMed

    Minamino, Tohru

    2018-06-01

    The bacterial flagellum is supramolecular motility machinery consisting of the basal body, the hook and the filament. Flagellar proteins are translocated across the cytoplasmic membrane via a type III protein export apparatus, diffuse down the central channel of the growing structure and assemble at the distal end. Flagellar assembly begins with the basal body, followed by the hook and finally the filament. The completion of hook assembly is the most important morphological checkpoint of the sequential flagellar assembly process. When the hook reaches its mature length of about 55 nm in Salmonella enterica, the type III protein export apparatus switches export specificity from proteins required for the structure and assembly of the hook to those responsible for filament assembly, thereby terminating hook assembly and initiating filament assembly. Three flagellar proteins, namely FliK, FlhB and FlhA, are responsible for this substrate specificity switching. Upon completion of the switching event, interactions among FlhA, the cytoplasmic ATPase complex and flagellar type III export chaperones establish the assembly order of the filament at the hook tip. Here, we describe our current understanding of a hierarchical protein export mechanism used in flagellar type III protein export.

  8. 7 CFR 35.12 - Inspection and certification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REGULATIONS EXPORT GRAPES AND PLUMS Regulations § 35.12 Inspection and certification. (a) Each person shipping or offering for shipment any variety of vinifera species table grapes, to any foreign destination... “Meets requirements of Export Grape and Plum Act” or (2) For any variety meeting specifications of...

  9. 15 CFR 762.3 - Records exempt from recordkeeping requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...; (2) Special export file list; (3) Vessel log from freight forwarder; (4) Inspection certificate; (5... form; (12) Financial hold form; (13) Export parts shipping problem form; (14) Draft number log; (15) Expense invoice mailing log; (16) Financial status report; (17) Bank release of guarantees; (18) Cash...

  10. 15 CFR 762.3 - Records exempt from recordkeeping requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...; (2) Special export file list; (3) Vessel log from freight forwarder; (4) Inspection certificate; (5... form; (12) Financial hold form; (13) Export parts shipping problem form; (14) Draft number log; (15) Expense invoice mailing log; (16) Financial status report; (17) Bank release of guarantees; (18) Cash...

  11. 78 FR 3423 - Agency Information Collection Activities: Final Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-16

    ... has placed additional reporting requirements on the Bank. By neutralizing the effect of export credit insurance and guarantees offered by foreign governments and by absorbing credit risks that the private... credit decision on approximately 85 export transactions per year in divisions dealing with aircraft...

  12. Frequent Questions about the Hazardous Waste Export-Import Revisions Final Rule

    EPA Pesticide Factsheets

    Answers questions such as: What new requirements did EPA finalize in the Hazardous Waste Export-Import Revisions Final Rule? Why did EPA implement these changes now? What are the benefits of the final rule? What are the compliance dates for the final rule?

  13. 19 CFR 192.2 - Requirements for exportation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... documentation describing the vehicle, which includes the Vehicle Identification Number or, if the vehicle does not have a Vehicle Identification Number, the product identification number. Exportation of a vehicle... Identification Number (VIN), the name of the owner or lienholder of the leased vehicle, and the telephone numbers...

  14. 19 CFR 192.2 - Requirements for exportation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... documentation describing the vehicle, which includes the Vehicle Identification Number or, if the vehicle does not have a Vehicle Identification Number, the product identification number. Exportation of a vehicle... Identification Number (VIN), the name of the owner or lienholder of the leased vehicle, and the telephone numbers...

  15. 19 CFR 192.2 - Requirements for exportation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... documentation describing the vehicle, which includes the Vehicle Identification Number or, if the vehicle does not have a Vehicle Identification Number, the product identification number. Exportation of a vehicle... Identification Number (VIN), the name of the owner or lienholder of the leased vehicle, and the telephone numbers...

  16. 7 CFR 1427.1204 - Eligible domestic users and exporters.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Cotton Competitiveness Payment Program § 1427.1204 Eligible domestic users and exporters. (a) For the... entered into an agreement with CCC to participate in the ELS Cotton Competitiveness Payment Program; or (2... Competitiveness Payment Program. (b) Payment applications must contain the documentation required by this subpart...

  17. 7 CFR 1493.510 - Payment for loss.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.510 Payment for loss. (a) Determination of CCC's liability. Upon receipt in good order of the information and documents required under § 1493.500, CCC will determine whether or not a...

  18. 7 CFR 1493.420 - Information required for program participation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC.... Before CCC will accept an application for a payment guarantee under the SCGP, the applicant must qualify... publicly available sources, CCC will determine whether the applicant is eligible for participation in the...

  19. 7 CFR 1493.510 - Payment for loss.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.510 Payment for loss. (a) Determination of CCC's liability. Upon receipt in good order of the information and documents required under § 1493.500, CCC will determine whether or not a...

  20. 7 CFR 1493.420 - Information required for program participation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC.... Before CCC will accept an application for a payment guarantee under the SCGP, the applicant must qualify... publicly available sources, CCC will determine whether the applicant is eligible for participation in the...

  1. 7 CFR 1493.510 - Payment for loss.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations § 1493.510 Payment for loss. (a) Determination of CCC's liability. Upon receipt in good order of the information and documents required under § 1493.500, CCC will determine whether or not a...

  2. 7 CFR 1493.420 - Information required for program participation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE EXPORT PROGRAMS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC.... Before CCC will accept an application for a payment guarantee under the SCGP, the applicant must qualify... publicly available sources, CCC will determine whether the applicant is eligible for participation in the...

  3. 31 CFR 515.542 - Mail and telecommunications-related transactions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of satellite radio or satellite television services to Cuba, or the entry into and performance under... authorize the entry into or performance of a contract with or for the benefit of any particular individual... news wire feeds. (g) Nothing in this section authorizes the exportation or re-exportation of any items...

  4. 31 CFR 515.542 - Mail and telecommunications-related transactions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of satellite radio or satellite television services to Cuba, or the entry into and performance under... authorize the entry into or performance of a contract with or for the benefit of any particular individual... news wire feeds. (g) Nothing in this section authorizes the exportation or re-exportation of any items...

  5. 31 CFR 515.542 - Mail and telecommunications-related transactions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of satellite radio or satellite television services to Cuba, or the entry into and performance under... authorize the entry into or performance of a contract with or for the benefit of any particular individual... news wire feeds. (g) Nothing in this section authorizes the exportation or re-exportation of any items...

  6. 76 FR 1400 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ... number. Animal and Plant Health Inspection Service Title: Certificate for Poultry and Hatching Eggs for... poultry. The export of agricultural commodities, including poultry and hatching eggs is a major business... import health requirements of other countries for poultry and hatching eggs exported from the U.S. Most...

  7. 7 CFR 1493.120 - Payment for loss.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Credit Guarantee Program (GSM-102) and CCC Intermediate Export Credit Guarantee Program (GSM-103... credit, or any obligation owed by the foreign bank to the assignee U.S. financial institution which is... with the reporting requirements under § 1493.80 and § 1493.90, excluding post-export adjustments (i.e...

  8. 15 CFR 30.9 - Transmitting and correcting Electronic Export Information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Transmitting and correcting Electronic Export Information. 30.9 Section 30.9 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade BUREAU OF THE CENSUS, DEPARTMENT OF COMMERCE FOREIGN TRADE REGULATIONS General Requirements...

  9. 15 CFR 30.37 - Miscellaneous exemptions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... of Electronic Export Information § 30.37 Miscellaneous exemptions. Filing EEI is not required for the...), exports of commodities where the value of the commodities shipped from one USPPI to one consignee on a... classification codes regardless of the total shipment value. In instances where a shipment contains a mixture of...

  10. 15 CFR 746.4 - North Korea.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... requiring a license are subject to case-by-case review, except as follows: (1) Luxury Goods. Applications to... No. 1 to part 746. (2) Applications to export or reexport arms and related materiel are subject to a general policy of denial. In addition, applications to export or reexport items specified by UN documents...

  11. 15 CFR 30.6 - Electronic Export Information data elements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... elements. 30.6 Section 30.6 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade... Electronic Export Information data elements. The information specified in this section is required for shipments transmitted to the AES. The data elements identified as “mandatory” shall be reported for each...

  12. 7 CFR 1530.107 - Bond or letter of credit requirements

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SERVICE, DEPARTMENT OF AGRICULTURE THE REFINED SUGAR RE-EXPORT PROGRAM, THE SUGAR CONTAINING PRODUCTS RE... export of sugar in sugar containing products, or the production of certain polyhydric alcohols, if the... product, or produce certain polyhydric alcohols may be the principal on the bond or letter of credit...

  13. 12 CFR 403.5 - Declassification and downgrading.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Declassification and downgrading. 403.5 Section 403.5 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES CLASSIFICATION, DECLASSIFICATION, AND... unlawfully classified, the Director may require the Export-Import Bank to declassify it. Any such decision by...

  14. 75 FR 1235 - Revisions to the Requirements for: Transboundary Shipments of Hazardous Wastes Between OECD...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-08

    ... of Spent Lead- Acid Batteries, Submitting Exception Reports for Export Shipments of Hazardous Wastes... of Spent Lead-Acid Batteries, Submitting Exception Reports for Export Shipments of Hazardous Wastes... batteries intended for reclamation in a foreign country, specify that all exception reports concerning...

  15. 40 CFR 80.1130 - Requirements for exporters of renewable fuels.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... gallons of renewable fuel. k = A discrete volume of renewable fuel. VOLk = The standardized volume of discrete volume k of exported renewable fuel, in gallons, calculated in accordance with § 80.1126(d)(7). EVk = The equivalence value associated with discrete volume k. = Sum involving all volumes of...

  16. 21 CFR 1313.05 - Requirements for an established business relationship.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... relationship. To document that an importer or exporter has an established business relationship with a customer... relationship. 1313.05 Section 1313.05 Food and Drugs DRUG ENFORCEMENT ADMINISTRATION, DEPARTMENT OF JUSTICE... street address of the chemical importer or exporter and of each regular customer; (b) The telephone...

  17. 50 CFR 23.36 - What are the requirements for an export permit?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Ginseng Appendix-I Plants Artificially Propagated for Commercial Purposes Biological Specimens Captive... noncommercial purposes. (See § 23.46 for the export of specimens that originated at a commercial breeding... IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIES OF WILD...

  18. 50 CFR 23.36 - What are the requirements for an export permit?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Ginseng Appendix-I Plants Artificially Propagated for Commercial Purposes Biological Specimens Captive... noncommercial purposes. (See § 23.46 for the export of specimens that originated at a commercial breeding... IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIES OF WILD...

  19. 50 CFR 23.36 - What are the requirements for an export permit?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Ginseng Appendix-I Plants Artificially Propagated for Commercial Purposes Biological Specimens Captive... noncommercial purposes. (See § 23.46 for the export of specimens that originated at a commercial breeding... IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIES OF WILD...

  20. 50 CFR 23.36 - What are the requirements for an export permit?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Ginseng Appendix-I Plants Artificially Propagated for Commercial Purposes Biological Specimens Captive... noncommercial purposes. (See § 23.46 for the export of specimens that originated at a commercial breeding... IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIES OF WILD...

  1. 50 CFR 15.12 - Requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., TRANSPORTATION, SALE, PURCHASE, BARTER, EXPORTATION, AND IMPORTATION OF WILDLIFE AND PLANTS WILD BIRD... States any exotic bird except as may be permitted under the terms of a valid permit issued pursuant to...) Any exotic bird can be imported to the United States if it was legally exported from the United States...

  2. 31 CFR 560.530 - Commercial sales, exportation, and reexportation of agricultural commodities, medicine, and...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... reexportation of agricultural commodities, medicine, and medical devices. 560.530 Section 560.530 Money and... commodities, medicine, and medical devices. (a)(1) One-year license requirement. (i) The exportation or reexportation of agricultural commodities, medicine, and medical devices that are not covered by the general...

  3. 31 CFR 560.530 - Commercial sales, exportation, and reexportation of agricultural commodities, medicine, and...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... reexportation of agricultural commodities, medicine, and medical devices. 560.530 Section 560.530 Money and... commodities, medicine, and medical devices. (a)(1) One-year license requirement. The exportation or... purchasers or importers, the excluded medicines specified in paragraph (a)(3)(iii) of this section, medicine...

  4. 21 CFR 312.110 - Import and export requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... drug has valid marketing authorization in Australia, Canada, Israel, Japan, New Zealand, Switzerland..., and § 1.101 of this chapter; or (3) The drug is being exported to Australia, Canada, Israel, Japan... specifications; (iv) The drug is not in conflict with the importing country's laws; (v) The outer shipping...

  5. 21 CFR 312.110 - Import and export requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... drug has valid marketing authorization in Australia, Canada, Israel, Japan, New Zealand, Switzerland..., and § 1.101 of this chapter; or (3) The drug is being exported to Australia, Canada, Israel, Japan... specifications; (iv) The drug is not in conflict with the importing country's laws; (v) The outer shipping...

  6. 21 CFR 312.110 - Import and export requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... drug has valid marketing authorization in Australia, Canada, Israel, Japan, New Zealand, Switzerland..., and § 1.101 of this chapter; or (3) The drug is being exported to Australia, Canada, Israel, Japan... specifications; (iv) The drug is not in conflict with the importing country's laws; (v) The outer shipping...

  7. Role of the mRNA export factor Sus1 in oxidative stress tolerance in Candida albicans.

    PubMed

    Xiao, Chenpeng; Yu, Qilin; Zhang, Bing; Li, Jianrong; Zhang, Dan; Li, Mingchun

    2018-02-05

    In eukaryotes, the nuclear export of mRNAs is essential for gene expression. However, little is known about the role of mRNA nuclear export in the important fungal pathogen, Candida albicans. In this study, we identified C. albicans Sus1, a nucleus-localized protein that is required for mRNA export. Interestingly, the sus1Δ/Δ displayed hyper-sensitivity to extracellular oxidative stress, enhanced ROS accumulation and severe oxidative stress-related cell death. More strikingly, although the mutant exhibited normal activation of the expression of oxidative stress response (OSR) genes, it had attenuated activity of ROS scavenging system, which may be attributed to the defect in OSR mRNA export in this mutant. In addition, the virulence of the sus1Δ/Δ was seriously attenuated. Taken together, our findings provide evidence that the mRNA export factor Sus1 plays an important role in oxidative stress tolerance and pathogenesis. Copyright © 2018 Elsevier Inc. All rights reserved.

  8. New insights into the structural and functional involvement of the gate loop in AcrB export activity.

    PubMed

    Ababou, Abdessamad

    2018-02-01

    AcrB is a major multidrug exporter in Escherichia coli and other Gram-negative bacteria. Its gate loop, located between the proximal and the distal pockets, have been reported to play important role in the export of many antibiotics. This loop location, rigidity and interactions with substrates have led recent reports to suggest that AcrB export mechanism operates in a sequential manner. First the substrate binds the proximal pocket in the access monomer, then it moves to bind the distal pocket in the binding monomer and subsequently it is extruded in the extrusion monomer. Recently, we have demonstrated that the gate loop is not required for the binding of Erythromycin but the integrity of this loop is important for an efficient export of this substrate. However, here we show that the antibiotic susceptibilities of the same AcrB gate loop mutants for Doxorubicin were unaffected, suggesting that this loop is not required for its export, and we demonstrate that this substrate may use principally the tunnel-1, located between transmembranes 8 and 9, more often than previously reported. To further explain our findings, here we address the gate loop mutations effects on AcrB solution energetics (fold, stability, molecular dynamics) and on the in vivo efflux of Erythromycin and Doxorubicin. Finally, we discuss the efflux and the discrepancy between the structural and the functional experiments for Erythromycin in these gate loop mutants. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. A Descriptive Study of the DoD-Managed Freight Forwarder Concept.

    DTIC Science & Technology

    1986-09-01

    AFLC/PMPO AMSAC-OL/ 7 AFLC ILC/ AWST MTMCEA/ ITEL AFLC ILC/EC MTMC-ITX AFLC ILC/GB USN/OP-632B AF IT! LSM USN/SUP-07C DISAM/DIC USASAC-NCAD (OL/T) SAAC...commercial insurance coverage for the protection of all purchaser-owned FMS materiel while in custody of the freight forwarder. r . Process FMS customer...export for each shipment. r . Provide export boxing and marking if required. s. Carry out freight rate negotiations as required. t. Retrieve freight from

  10. Cellular Nuclear Export Factors TAP and Aly Are Required for HDAg-L-mediated Assembly of Hepatitis Delta Virus.

    PubMed

    Huang, Hsiu-Chen; Lee, Chung-Pei; Liu, Hui-Kang; Chang, Ming-Fu; Lai, Yu-Heng; Lee, Yu-Ching; Huang, Cheng

    2016-12-09

    Hepatitis delta virus (HDV) is a satellite virus of hepatitis B virus (HBV). HDV genome encodes two forms of hepatitis delta antigen (HDAg), small HDAg (HDAg-S), which is required for viral replication, and large HDAg (HDAg-L), which is essential for viral assembly. HDAg-L is identical to HDAg-S except that it bears a 19-amino acid extension at the C terminus. Both HDAgs contain a nuclear localization signal (NLS), but only HDAg-L contains a CRM1-independent nuclear export signal at its C terminus. The nuclear export activity of HDAg-L is important for HDV particle formation. However, the mechanisms of HDAg-L-mediated nuclear export of HDV ribonucleoprotein are not clear. In this study, the host cellular RNA export complex TAP-Aly was found to form a complex with HDAg-L, but not with an export-defective HDAg-L mutant, in which Pro 205 was replaced by Ala. HDAg-L was found to colocalize with TAP and Aly in the nucleus. The C-terminal domain of HDAg-L was shown to directly interact with the N terminus of TAP, whereas an HDAg-L mutant lacking the NLS failed to interact with full-length TAP. In addition, small hairpin RNA-mediated down-regulation of TAP or Aly reduced nuclear export of HDAg-L and assembly of HDV virions. Furthermore, a peptide, TAT-HDAg-L(198-210), containing the 10-amino acid TAT peptide and HDAg-L(198-210), inhibited the interaction between HDAg-L and TAP and blocked HDV virion assembly and secretion. These data demonstrate that formation and release of HDV particles are mediated by TAP and Aly. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  11. Nrt1 and Tna1-Independent Export of NAD+ Precursor Vitamins Promotes NAD+ Homeostasis and Allows Engineering of Vitamin Production

    PubMed Central

    Belenky, Peter; Stebbins, Rebecca; Bogan, Katrina L.; Evans, Charles R.; Brenner, Charles

    2011-01-01

    NAD+ is both a co-enzyme for hydride transfer enzymes and a substrate of sirtuins and other NAD+ consuming enzymes. NAD+ biosynthesis is required for two different regimens that extend lifespan in yeast. NAD+ is synthesized from tryptophan and the three vitamin precursors of NAD+: nicotinic acid, nicotinamide and nicotinamide riboside. Supplementation of yeast cells with NAD+ precursors increases intracellular NAD+ levels and extends replicative lifespan. Here we show that both nicotinamide riboside and nicotinic acid are not only vitamins but are also exported metabolites. We found that the deletion of the nicotinamide riboside transporter, Nrt1, leads to increased export of nicotinamide riboside. This discovery was exploited to engineer a strain to produce high levels of extracellular nicotinamide riboside, which was recovered in purified form. We further demonstrate that extracellular nicotinamide is readily converted to extracellular nicotinic acid in a manner that requires intracellular nicotinamidase activity. Like nicotinamide riboside, export of nicotinic acid is elevated by the deletion of the nicotinic acid transporter, Tna1. The data indicate that NAD+ metabolism has a critical extracellular element in the yeast system and suggest that cells regulate intracellular NAD+ metabolism by balancing import and export of NAD+ precursor vitamins. PMID:21589930

  12. Nrt1 and Tna1-independent export of NAD+ precursor vitamins promotes NAD+ homeostasis and allows engineering of vitamin production.

    PubMed

    Belenky, Peter; Stebbins, Rebecca; Bogan, Katrina L; Evans, Charles R; Brenner, Charles

    2011-05-11

    NAD(+) is both a co-enzyme for hydride transfer enzymes and a substrate of sirtuins and other NAD(+) consuming enzymes. NAD(+) biosynthesis is required for two different regimens that extend lifespan in yeast. NAD(+) is synthesized from tryptophan and the three vitamin precursors of NAD(+): nicotinic acid, nicotinamide and nicotinamide riboside. Supplementation of yeast cells with NAD(+) precursors increases intracellular NAD(+) levels and extends replicative lifespan. Here we show that both nicotinamide riboside and nicotinic acid are not only vitamins but are also exported metabolites. We found that the deletion of the nicotinamide riboside transporter, Nrt1, leads to increased export of nicotinamide riboside. This discovery was exploited to engineer a strain to produce high levels of extracellular nicotinamide riboside, which was recovered in purified form. We further demonstrate that extracellular nicotinamide is readily converted to extracellular nicotinic acid in a manner that requires intracellular nicotinamidase activity. Like nicotinamide riboside, export of nicotinic acid is elevated by the deletion of the nicotinic acid transporter, Tna1. The data indicate that NAD(+) metabolism has a critical extracellular element in the yeast system and suggest that cells regulate intracellular NAD(+) metabolism by balancing import and export of NAD(+) precursor vitamins.

  13. Nmd3p Is a Crm1p-Dependent Adapter Protein for Nuclear Export of the Large Ribosomal Subunit

    PubMed Central

    Ho, Jennifer Hei-Ngam; Kallstrom, George; Johnson, Arlen W.

    2000-01-01

    In eukaryotic cells, nuclear export of nascent ribosomal subunits through the nuclear pore complex depends on the small GTPase Ran. However, neither the nuclear export signals (NESs) for the ribosomal subunits nor the receptor proteins, which recognize the NESs and mediate export of the subunits, have been identified. We showed previously that Nmd3p is an essential protein from yeast that is required for a late step in biogenesis of the large (60S) ribosomal subunit. Here, we show that Nmd3p shuttles and that deletion of the NES from Nmd3p leads to nuclear accumulation of the mutant protein, inhibition of the 60S subunit biogenesis, and inhibition of the nuclear export of 60S subunits. Moreover, the 60S subunits that accumulate in the nucleus can be coimmunoprecipitated with the NES-deficient Nmd3p. 60S subunit biogenesis and export of truncated Nmd3p were restored by the addition of an exogenous NES. To identify the export receptor for Nmd3p we show that Nmd3p shuttling and 60S export is blocked by the Crm1p-specific inhibitor leptomycin B. These results identify Crm1p as the receptor for Nmd3p export. Thus, export of the 60S subunit is mediated by the adapter protein Nmd3p in a Crm1p-dependent pathway. PMID:11086007

  14. Absorptive capacity of Libya: a constrained expenditure approach. [Policy of max-minimize oil exports

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    El-Jehaimi, T.

    This paper is concerned with estimating the absorptive capacity of Libya as it relates to the country's development objectives. Specifically, the paper will attempt, on the bases of analytical assumptions and a model of econometric relationships, to estimate the levels of oil exports which will be required in order to finance the economic development of Libya over the next ten years. By emphasizing the requirements of domestic development, the question of surplus will not arise and, unlike in some other works, the absorption problem will not be met. This approach, explained in detail, should give more realistic results since itmore » has been the policy of Libya to max-minimize oil exports since 1970, i.e., to export high enough to pay for imports and other foreign obligations such as foreign aid but low enough to not generate excess revenues. Excess revenues are revenues in excess of what is required to maintain the reserves/imports ratio at an optimum level. The conservation policy adopted by Libya has resulted in lowering production levels from a peak of 3.2 million barrels per day (mb/d) in 1970 to a little over 1 mb/d in 1975. This policy is expected to continue. (From Introduction)« less

  15. Review: transport of tRNA out of the nucleus-direct channeling to the ribosome?

    PubMed

    Grosshans, H; Simos, G; Hurt, E

    2000-04-01

    Although tRNA was the first substrate whose export from the nuclei of eukaryotic cells had been shown to be carrier-mediated and active, it has only been in the last 2 years that the first mechanistic details of this nucleocytoplasmic transport pathway have begun to emerge. A member of the importin/karyopherin beta superfamily, Los1p in yeast and Xpo-t in vertebrates, has been shown to export tRNA in cooperation with the small GTPase Ran (Gsp1p) from the nucleus into the cytoplasm, where tRNA becomes available for translation. However, Los1p is not essential for viability in yeast cells, suggesting that alternative tRNA export pathways exist. Recent results show that aminoacylation and a translation factor are also required for efficient nuclear tRNA export. Thus, protein translation and nuclear export of tRNA appear to be coupled processes. Copyright 2000 Academic Press.

  16. The models for financial crisis detection in Indonesia based on import, export, and foreign exchange reserves

    NASA Astrophysics Data System (ADS)

    Sugiyanto; Wibowo, Supriyadi; Rizky Aristina Suwardi, Vivi

    2017-12-01

    The severity of the financial crisis that occurred in Indonesia required an early warning system of financial crisis. The financial crisis in Indonesia can be detected based on imports, exports, and foreign exchange reserves. The purpose of the research is to determine an appropriate model to detect the financial crisis in Indonesia based on imports, exports, and foreign exchange reserves. Markov switching is an alternative framework for the approach often used in financial crisis detection. Combined volatility and Markov switching model with three states assumptions can be established if an AR and volatility models have been obtained. Imports, exports, and foreign exchange reserves data from January 1990 to December 2016 have the heteroscedasticity effect so that an ARCH model is used as a volatility model. Research shows that SWARCH(3.1) model is an appropriate model for detecting financial crisis in Indonesia based on imports, exports, and foreign exchange reserves.

  17. Natural gas imports and exports. Fourth quarter report, 1998

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1998-12-31

    The Office of Natural Gas and Petroleum Import and Export Activities prepares quarterly reports summarizing the data provided by companies authorized to import or export natural gas. Companies are required, as a condition of their authorizations, to file quarterly reports. This report is for the fourth quarter of 1998 (October through December). Attachment A shows the percentage of takes to maximum firm contract levels and the weighted average per unit price for each of the long-term importers during the five most recent reporting quarters. Attachment B shows volumes and prices of gas purchased by long-term importers and exporters during themore » past 12 months. Attachment C shows volume and price information pertaining to gas imported on a short-term or spot market basis. Attachment D shows the gas exported on a short-term or spot market basis to Canada and Mexico.« less

  18. Natural gas imports and exports. First quarter report, 1998

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    The Office of Natural Gas and Petroleum Import and Export Activities prepares quarterly reports summarizing the data provided by companies authorized to import or export natural gas. Companies are required, as a condition of their authorizations, to file quarterly reports. This report is for the first quarter of 1998 (January through March). Attachment A shows the percentage of takes to maximum firm contract levels and the weighted average per unit price for each of the long-term importers during the five most recent reporting quarters. Attachment B shows volumes and prices of gas purchased by long-term importers and exporters during themore » past 12 months. Attachment C shows volume and price information pertaining to gas imported on a short-term or spot market basis. Attachment D shows the gas exported on a short-term or spot market basis to Canada and Mexico.« less

  19. Regulatory aspects of pharmaceuticals' exports in gulf cooperation council countries.

    PubMed

    Pateriya, S; Janodia, Md; Deshpande, Pb; Ligade, Vs; Talole, Kb; Kulshrestha, T; Kamariya, Y; Musmade, Pb; Udupa, N

    2011-04-01

    The Gulf cooperation council (GCC) region is considered as "Emerging market" for pharmaceutical export and bilateral trade. The understanding of the regulatory requirements of this region can be beneficial for pharmaceutical export. Some incidents of the year 2008-09, like recession or economic slowdown in highly well-off and regulated market of the EU and US, raised the demand for alternate destinations for business. The regulations of Gulf countries are encouraging the import of quality generic products, which can be good news to the Indian drug manufacturers.

  20. Regulatory Aspects of Pharmaceuticals’ Exports in Gulf Cooperation Council Countries

    PubMed Central

    Pateriya, S; Janodia, MD; Deshpande, PB; Ligade, VS; Talole, KB; Kulshrestha, T; Kamariya, Y; Musmade, PB; Udupa, N

    2011-01-01

    The Gulf cooperation council (GCC) region is considered as “Emerging market” for pharmaceutical export and bilateral trade. The understanding of the regulatory requirements of this region can be beneficial for pharmaceutical export. Some incidents of the year 2008-09, like recession or economic slowdown in highly well-off and regulated market of the EU and US, raised the demand for alternate destinations for business. The regulations of Gulf countries are encouraging the import of quality generic products, which can be good news to the Indian drug manufacturers. PMID:21731362

  1. 75 FR 82463 - Yuri I. Montgomery, Respondent; Final Decision and Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-30

    ... orders; that all exhibits except Ex. 7 are dated prior to the discovery deadline and are thus untimely... Ex. B) 2. Specifically, Respondent's conviction was for knowingly and willingly exporting and causing... for and obtaining the required export licenses from the Department of Commerce. (BIS Ex. B) 3. Section...

  2. 19 CFR 10.248 - Additional requirements for preferential treatment of brassieres.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the port of exportation; (C) In the case of fabric components purchased by the producer or entity... expenses for cutting or other processing to create the fabric components other than knitting to shape, that... shape, that the producer or entity controlling production can verify; or (2) If no exportation to an...

  3. 19 CFR 10.228 - Additional requirements for preferential treatment of brassieres.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the port of exportation; (C) In the case of fabric components purchased by the producer or entity... expenses for cutting or other processing to create the fabric components other than knitting to shape, that... shape, that the producer or entity controlling production can verify; or (2) If no exportation to a...

  4. 19 CFR 10.228 - Additional requirements for preferential treatment of brassieres.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the port of exportation; (C) In the case of fabric components purchased by the producer or entity... expenses for cutting or other processing to create the fabric components other than knitting to shape, that... shape, that the producer or entity controlling production can verify; or (2) If no exportation to a...

  5. 19 CFR 10.248 - Additional requirements for preferential treatment of brassieres.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the port of exportation; (C) In the case of fabric components purchased by the producer or entity... expenses for cutting or other processing to create the fabric components other than knitting to shape, that... shape, that the producer or entity controlling production can verify; or (2) If no exportation to an...

  6. 15 CFR 746.2 - Cuba.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Cuba. 746.2 Section 746.2 Commerce and... § 746.2 Cuba. (a) License requirements. As authorized by section 6 of the Export Administration Act of... subject to the EAR) to Cuba, except as follows. (1) License Exceptions. You may export or reexport without...

  7. 15 CFR 746.2 - Cuba.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Cuba. 746.2 Section 746.2 Commerce and... § 746.2 Cuba. (a) License requirements. As authorized by section 6 of the Export Administration Act of... subject to the EAR) to Cuba, except as follows. (1) License Exceptions. You may export or reexport without...

  8. 15 CFR 746.2 - Cuba.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Cuba. 746.2 Section 746.2 Commerce and... § 746.2 Cuba. (a) License requirements. As authorized by section 6 of the Export Administration Act of... subject to the EAR) to Cuba, except as follows. (1) License Exceptions. You may export or reexport without...

  9. 15 CFR 746.2 - Cuba.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Cuba. 746.2 Section 746.2 Commerce and... § 746.2 Cuba. (a) License requirements. As authorized by section 6 of the Export Administration Act of... subject to the EAR) to Cuba, except as follows. (1) License Exceptions. You may export or reexport without...

  10. 15 CFR 746.2 - Cuba.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Cuba. 746.2 Section 746.2 Commerce and... § 746.2 Cuba. (a) License requirements. As authorized by section 6 of the Export Administration Act of... subject to the EAR) to Cuba, except as follows. (1) License Exceptions. You may export or reexport without...

  11. Impacts of U.S. Export Control Policies on Science and Technology Activities and Competitiveness

    DTIC Science & Technology

    2009-02-25

    coffee table. However, under the current export control regime, the stand was considered ‘ITAR hardware’ and we were required to have two security...should survive without an effective method for pruning items from the control lists when they no longer serve a significant definable national

  12. 9 CFR 130.20 - User fees for endorsing export certificates.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... vaccinations are listed in the following table. The user fees apply to each export health certificate 5... health certificates that require the verification of tests or vaccinations are listed in the following... on the number of animals or birds covered by the certificate and the number of tests or vaccinations...

  13. 9 CFR 130.20 - User fees for endorsing export certificates.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... vaccinations are listed in the following table. The user fees apply to each export health certificate 5... health certificates that require the verification of tests or vaccinations are listed in the following... on the number of animals or birds covered by the certificate and the number of tests or vaccinations...

  14. 9 CFR 130.20 - User fees for endorsing export certificates.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... vaccinations are listed in the following table. The user fees apply to each export health certificate 5... health certificates that require the verification of tests or vaccinations are listed in the following... on the number of animals or birds covered by the certificate and the number of tests or vaccinations...

  15. 27 CFR 28.302 - Notice to exporter.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... been lost by theft, he will advise the exporter by letter: (a) Of the identity of the containers; (b) Of the amount of the loss; (c) Of the circumstances indicating diversion or theft; (d) That allowance... transportation, as described in § 28.301, whether by theft or otherwise, the appropriate TTB officer may require...

  16. 27 CFR 28.302 - Notice to exporter.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... been lost by theft, he will advise the exporter by letter: (a) Of the identity of the containers; (b) Of the amount of the loss; (c) Of the circumstances indicating diversion or theft; (d) That allowance... transportation, as described in § 28.301, whether by theft or otherwise, the appropriate TTB officer may require...

  17. 19 CFR 144.37 - Withdrawal for exportation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Form 7512 or CBP Form 7501 must contain all of the statistical information as provided in § 141.61(e... exportation. (e) Weight, gauge, or measure. Merchandise in bulk and packaged articles which are customarily... excess of 4 liters and cigarettes in quantities in excess of 3 cartons. Also, the address requirement is...

  18. 50 CFR 23.46 - What are the requirements for registering a commercial breeding operation for Appendix-I wildlife...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... a commercial breeding operation for Appendix-I wildlife and commercially exporting specimens? 23.46... registering a commercial breeding operation for Appendix-I wildlife and commercially exporting specimens? (a... originating from a commercial breeding operation that is registered with the CITES Secretariat may be traded...

  19. 50 CFR 23.36 - What are the requirements for an export permit?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Ginseng 3-200-34 Appendix-I Plants Artificially Propagated for Commercial Purposes 3-200-33 Biological... noncommercial purposes. (See § 23.46 for the export of specimens that originated at a commercial breeding... IMPORTATION OF WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIES OF WILD...

  20. 50 CFR 23.46 - What are the requirements for registering a commercial breeding operation for Appendix-I wildlife...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... a commercial breeding operation for Appendix-I wildlife and commercially exporting specimens? 23.46... registering a commercial breeding operation for Appendix-I wildlife and commercially exporting specimens? (a... originating from a commercial breeding operation that is registered with the CITES Secretariat may be traded...

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