Material Concerns: Evaluating Sulfur Concrete for use in the Lunar Environment

NASA Technical Reports Server (NTRS)

Grugel, Richard N.; Toutanji, Houssam

2006-01-01

On Earth sulfur "concrete" is an established construction material that has good mechanical properties, generally better than Portland cement, and can be used in corrosive environments. Troilite (FeS) has been found on the moon and raises the question of using extracted sulfur as a lunar construction material, an attractive alternative to conventional concrete as it does not require water. Troilite reduction to elemental sulfur and using it to make concrete in a lunar setting has been previously discussed. However, little has been experimentally done to evaluate its performance in the extreme lunar environment. This study subjected sets of sulfur concrete samples, prepared using JSC-1 lunar simulant, to I ) extended periods of high vacuum and 2) extreme temperature cycles. Here an overview of sulfur concrete and experimentally assessed properties, put in context of the lunar environment, is presented and discussed.

Hydrocarbon biodegradation in hypersaline environments.

PubMed

Ward, D M; Brock, T D

1978-02-01

When mineral oil, hexadecane, and glutamate were added to natural samples of varying salinity (3.3 to 28.4%) from salt evaporation ponds and Great Salt Lake, Utah, rates of metabolism of these compounds decreased as salinity increased. Rate limitations did not appear to relate to low oxygen levels or to the availability of organic nutrients. Some oxidation of l-[U-C]glutamic acid occurred even at extreme salinities, whereas oxidation of [1-C]hexadecane was too low to be detected. Gas chromatographic examination of hexane-soluble components of tar samples from natural seeps at Rozel Point in Great Salt Lake demonstrated no evidence of biological oxidation of isoprenoid alkanes subject to degradation in normal environments. Some hexane-soluble components of the same tar were altered by incubation in a low-salinity enrichment culture inoculated with garden soil. Attempts to enrich for microorganisms in saline waters able to use mineral oil as a sole source of carbon and energy were successful below, but not above, about 20% salinity. This study strongly suggests a general reduction of metabolic rate at extreme salinities and raises doubt about the biodegradation of hydrocarbons in hypersaline environments.

Progress Towards providing Heat-Shield for Extreme Entry Environment Technology (HEEET) for Venus and other New Froniters Missions

NASA Technical Reports Server (NTRS)

Venkatapathy, Ethiraj; Ellerby, Don; Gage, Peter

2017-01-01

Heat-shield for Extreme Entry Environment Technology (HEEET) has been in development since 2014 with the goal of enabling missions to Venus, Saturn and other high-speed sample return missions. It is offered as a new technology and incentivized for mission use in the New Frontiers 4 AO by NASA. The current plans are to mature the technology to TRL 6 by FY18. The HEEET Team has been working closely with multiple NF-4 proposals to Venus, Saturn and has been supporting recent Ice-Giants mission studies. This presentation will provide progress made to date and the plans for development in FY18.

The ecology and diversity of microbial eukaryotes in geothermal springs.

PubMed

Oliverio, Angela M; Power, Jean F; Washburne, Alex; Cary, S Craig; Stott, Matthew B; Fierer, Noah

2018-04-16

Decades of research into the Bacteria and Archaea living in geothermal spring ecosystems have yielded great insight into the diversity of life and organismal adaptations to extreme environmental conditions. Surprisingly, while microbial eukaryotes (protists) are also ubiquitous in many environments, their diversity across geothermal springs has mostly been ignored. We used high-throughput sequencing to illuminate the diversity and structure of microbial eukaryotic communities found in 160 geothermal springs with broad ranges in temperature and pH across the Taupō Volcanic Zone in New Zealand. Protistan communities were moderately predictable in composition and varied most strongly across gradients in pH and temperature. Moreover, this variation mirrored patterns observed for bacterial and archaeal communities across the same spring samples, highlighting that there are similar ecological constraints across the tree of life. While extreme pH values were associated with declining protist diversity, high temperature springs harbored substantial amounts of protist diversity. Although protists are often overlooked in geothermal springs and other extreme environments, our results indicate that such environments can host distinct and diverse protistan communities.

Tropical Aquatic Archaea Show Environment-Specific Community Composition

PubMed Central

Silveira, Cynthia B.; Cardoso, Alexander M.; Coutinho, Felipe H.; Lima, Joyce L.; Pinto, Leonardo H.; Albano, Rodolpho M.; Clementino, Maysa M.; Martins, Orlando B.; Vieira, Ricardo P.

2013-01-01

The Archaea domain is ubiquitously distributed and extremely diverse, however, environmental factors that shape archaeal community structure are not well known. Aquatic environments, including the water column and sediments harbor many new uncultured archaeal species from which metabolic and ecological roles remain elusive. Some environments are especially neglected in terms of archaeal diversity, as is the case of pristine tropical areas. Here we investigate the archaeal composition in marine and freshwater systems from Ilha Grande, a South Atlantic tropical environment. All sampled habitats showed high archaeal diversity. No OTUs were shared between freshwater, marine and mangrove sediment samples, yet these environments are interconnected and geographically close, indicating environment-specific community structuring. Group II Euryarchaeota was the main clade in marine samples, while the new putative phylum Thaumarchaeota and LDS/RCV Euryarchaeota dominated freshwaters. Group III Euryarchaeota , a rare clade, was also retrieved in reasonable abundance in marine samples. The archaeal community from mangrove sediments was composed mainly by members of mesophilic Crenarchaeota and by a distinct clade forming a sister-group to Crenarchaeota and Thaumarchaeota. Our results show strong environment-specific community structuring in tropical aquatic Archaea, as previously seen for Bacteria. PMID:24086729

Extreme Material Physical Properties and Measurements above 100 tesla

NASA Astrophysics Data System (ADS)

Mielke, Charles

2011-03-01

The National High Magnetic Field Laboratory (NHMFL) Pulsed Field Facility (PFF) at Los Alamos National Laboratory (LANL) offers extreme environments of ultra high magnetic fields above 100 tesla by use of the Single Turn method as well as fields approaching 100 tesla with more complex methods. The challenge of metrology in the extreme magnetic field generating devices is complicated by the millions of amperes of current and tens of thousands of volts that are required to deliver the pulsed power needed for field generation. Methods of detecting physical properties of materials are essential parts of the science that seeks to understand and eventually control the fundamental functionality of materials in extreme environments. De-coupling the signal of the sample from the electro-magnetic interference associated with the magnet system is required to make these state-of-the-art magnetic fields useful to scientists studying materials in high magnetic fields. The cutting edge methods that are being used as well as methods in development will be presented with recent results in Graphene and High-Tc superconductors along with the methods and challenges. National Science Foundation DMR-Award 0654118.

The Janus face of Darwinian competition

PubMed Central

Hintze, Arend; Phillips, Nathaniel; Hertwig, Ralph

2015-01-01

Without competition, organisms would not evolve any meaningful physical or cognitive abilities. Competition can thus be understood as the driving force behind Darwinian evolution. But does this imply that more competitive environments necessarily evolve organisms with more sophisticated cognitive abilities than do less competitive environments? Or is there a tipping point at which competition does more harm than good? We examine the evolution of decision strategies among virtual agents performing a repetitive sampling task in three distinct environments. The environments differ in the degree to which the actions of a competitor can affect the fitness of the sampling agent, and in the variance of the sample. Under weak competition, agents evolve decision strategies that sample often and make accurate decisions, which not only improve their own fitness, but are good for the entire population. Under extreme competition, however, the dark side of the Janus face of Darwinian competition emerges: Agents are forced to sacrifice accuracy for speed and are prevented from sampling as often as higher variance in the environment would require. Modest competition is therefore a good driver for the evolution of cognitive abilities and of the population as a whole, whereas too much competition is devastating. PMID:26354182

The Janus face of Darwinian competition.

PubMed

Hintze, Arend; Phillips, Nathaniel; Hertwig, Ralph

2015-09-10

Without competition, organisms would not evolve any meaningful physical or cognitive abilities. Competition can thus be understood as the driving force behind Darwinian evolution. But does this imply that more competitive environments necessarily evolve organisms with more sophisticated cognitive abilities than do less competitive environments? Or is there a tipping point at which competition does more harm than good? We examine the evolution of decision strategies among virtual agents performing a repetitive sampling task in three distinct environments. The environments differ in the degree to which the actions of a competitor can affect the fitness of the sampling agent, and in the variance of the sample. Under weak competition, agents evolve decision strategies that sample often and make accurate decisions, which not only improve their own fitness, but are good for the entire population. Under extreme competition, however, the dark side of the Janus face of Darwinian competition emerges: Agents are forced to sacrifice accuracy for speed and are prevented from sampling as often as higher variance in the environment would require. Modest competition is therefore a good driver for the evolution of cognitive abilities and of the population as a whole, whereas too much competition is devastating.

Biomarkers and taphonomic processes in fresh and fossil biosignatures from Hot Spring silica deposits in El Tatio Chile, as a Mars Analogue

NASA Astrophysics Data System (ADS)

Carrizo, D.; Sánchez-García, L.; Parro, V.; Cady, S. L.; Cabrol, N. A.

2017-09-01

Biomarkers characterization and taphonomic process of recent and fossil biosignatures in extreme environments with analogies to Mars is essential to understanding how life could develop and survive in this conditions. Siliceous sinter deposits on Mars where similar to those found in the hydrothermal hot springs and geysers from El Tatio, Chile. Organic preservation have been shown in this study. Many different labile functional groups (i.e., carboxylic acids, alcohols, aldehydes, etc.) were found in both "age" samples. A shift in congener pattern for the different lipids families were found and discuss. This results give insight in taphonomic processes actin in this extreme environment, which could be used as a baseline in Mars exploration.

Extremely halophilic archaea and the issue of long-term microbial survival

PubMed Central

2011-01-01

Halophilic archaebacteria (haloarchaea) thrive in environments with salt concentrations approaching saturation, such as natural brines, the Dead Sea, alkaline salt lakes and marine solar salterns; they have also been isolated from rock salt of great geological age (195–250 million years). An overview of their taxonomy, including novel isolates from rock salt, is presented here; in addition, some of their unique characteristics and physiological adaptations to environments of low water activity are reviewed. The issue of extreme long-term microbial survival is considered and its implications for the search for extraterrestrial life. The development of detection methods for subterranean haloarchaea, which might also be applicable to samples from future missions to space, is presented. PMID:21984879

Optimizing the triple-axis spectrometer PANDA at the MLZ for small samples and complex sample environment conditions

NASA Astrophysics Data System (ADS)

Utschick, C.; Skoulatos, M.; Schneidewind, A.; Böni, P.

2016-11-01

The cold-neutron triple-axis spectrometer PANDA at the neutron source FRM II has been serving an international user community studying condensed matter physics problems. We report on a new setup, improving the signal-to-noise ratio for small samples and pressure cell setups. Analytical and numerical Monte Carlo methods are used for the optimization of elliptic and parabolic focusing guides. They are placed between the monochromator and sample positions, and the flux at the sample is compared to the one achieved by standard monochromator focusing techniques. A 25 times smaller spot size is achieved, associated with a factor of 2 increased intensity, within the same divergence limits, ± 2 ° . This optional neutron focusing guide shall establish a top-class spectrometer for studying novel exotic properties of matter in combination with more stringent sample environment conditions such as extreme pressures associated with small sample sizes.

Hydrocarbon Biodegradation in Hypersaline Environments

PubMed Central

Ward, David M.; Brock, T. D.

1978-01-01

When mineral oil, hexadecane, and glutamate were added to natural samples of varying salinity (3.3 to 28.4%) from salt evaporation ponds and Great Salt Lake, Utah, rates of metabolism of these compounds decreased as salinity increased. Rate limitations did not appear to relate to low oxygen levels or to the availability of organic nutrients. Some oxidation of l-[U-14C]glutamic acid occurred even at extreme salinities, whereas oxidation of [1-14C]hexadecane was too low to be detected. Gas chromatographic examination of hexane-soluble components of tar samples from natural seeps at Rozel Point in Great Salt Lake demonstrated no evidence of biological oxidation of isoprenoid alkanes subject to degradation in normal environments. Some hexane-soluble components of the same tar were altered by incubation in a low-salinity enrichment culture inoculated with garden soil. Attempts to enrich for microorganisms in saline waters able to use mineral oil as a sole source of carbon and energy were successful below, but not above, about 20% salinity. This study strongly suggests a general reduction of metabolic rate at extreme salinities and raises doubt about the biodegradation of hydrocarbons in hypersaline environments. PMID:16345276

Study on the interaction mechanism between the special geological environment and their extreme geo-microbes in Dagang Oilfield by combined methods

NASA Astrophysics Data System (ADS)

Yao, Jun

2010-05-01

Geo-microbes and their function were widespread ever since life appeared on the earth. Geo-microbiological process has left a rich and colorful material record in the geological body of earth, the most critical record of which is all sorts of organic hieroglyph and various forms of organic matter derived from bio-organisms, and oil field is the most ideal geological location to preserve these organic matters. It have already produced or might produce petroleum and natural gas sedimentary rocks under natural conditions, also known as olefiant (gas) rock or the parent rock, which is the product of the interaction between the life-system and earth environmental system in the specific geological conditions and integrate the whole microbial ecosystem in the geological time. The microbial community under extreme geological environment of Dagang Oilfield is relatively simple, therefore it is quite easy to investigate the special relationship between geo-microbes and biogeochemistry. We have mastered a large number of information related with the geological condition and biological species of Dagang Oilfield; what's more we also have isolated a number of archimycetes strains with different extremophiles capacity from the core samples collected in the Dagang oil field. At present, we are to proceed with the cooperative research at Environment School of Yale University and Institute of the Earth's biosphere using these strains. In the future, we will work together to carry out geological surveys in the field using international first-class equipment and methods and study the geological environment of Dagang Oilfield utilizing isotope techniques and mineral phase analysis method. Meanwhile we are going to undertake the on-line monitoring of the overall microbial activity of these collected geological samples, the specific metabolic activity of these extreme strains of microorganisms and the biomarkers produced during their metabolic processes under laboratory conditions. According to these research work listed above, we can reveal the mechanism of interaction between the special geological environment of Dagang Oilfield and the extreme geo-microbes, so as to clarify the effects of oil field environment on the extreme geo-microbes and especially the adverse effect of these geo-microbes to the geological environment, which may provide a practical foundation of theoretical basis for the reasons why the Dagang Oilfield can produce oil. Acknowledgement This work was supported in part by grants from National Outstanding Youth Research Foundation of China (40925010), International Joint Key Project from National Natural Science Foundation of China (40920134003), National Natural Science Foundation of China (40873060), and International Joint Key Project from Chinese Ministry of Science and Technology (2009DFA92830), and the 111 Project (08030).

Extreme Environment Simulation - Current and New Capabilities to Simulate Venus and Other Planetary Bodies

NASA Technical Reports Server (NTRS)

Kremic, Tibor; Vento, Dan; Lalli, Nick; Palinski, Timothy

2014-01-01

Science, technology, and planetary mission communities have a growing interest in components and systems that are capable of working in extreme (high) temperature and pressure conditions. Terrestrial applications range from scientific research, aerospace, defense, automotive systems, energy storage and power distribution, deep mining and others. As the target environments get increasingly extreme, capabilities to develop and test the sensors and systems designed to operate in such environments will be required. An application of particular importance to the planetary science community is the ability for a robotic lander to survive on the Venus surface where pressures are nearly 100 times that of Earth and temperatures approach 500C. The scientific importance and relevance of Venus missions are stated in the current Planetary Decadal Survey. Further, several missions to Venus were proposed in the most recent Discovery call. Despite this interest, the ability to accurately simulate Venus conditions at a scale that can test and validate instruments and spacecraft systems and accurately simulate the Venus atmosphere has been lacking. This paper discusses and compares the capabilities that are known to exist within and outside the United States to simulate the extreme environmental conditions found in terrestrial or planetary surfaces including the Venus atmosphere and surface. The paper then focuses on discussing the recent additional capability found in the NASA Glenn Extreme Environment Rig (GEER). The GEER, located at the NASA Glenn Research Center in Cleveland, Ohio, is designed to simulate not only the temperature and pressure extremes described, but can also accurately reproduce the atmospheric compositions of bodies in the solar system including those with acidic and hazardous elements. GEER capabilities and characteristics are described along with operational considerations relevant to potential users. The paper presents initial operating results and concludes with a sampling of investigations or tests that have been requested or expected.

Cleanroom Robotics: Appropriate Technology for a Sample Receiving Facility?

NASA Technical Reports Server (NTRS)

Bell, M. S.; Allen, C. C.

2005-01-01

NASA is currently pursuing a vigorous program that will collect samples from a variety of solar system environments. The Mars Exploration Program is expected to launch spacecraft that are designed to collect samples of martian soil, rocks, and atmosphere and return them to Earth, perhaps as early as 2016. International treaty obligations mandate that NASA conduct such a program in a manner that avoids cross-contamination both Earth and Mars. Because of this requirement, Mars sample curation will require a high degree biosafety, combined with extremely low levels inorganic, organic, and biological contamination.

A metagenomic framework for the study of airborne microbial communities.

PubMed

Yooseph, Shibu; Andrews-Pfannkoch, Cynthia; Tenney, Aaron; McQuaid, Jeff; Williamson, Shannon; Thiagarajan, Mathangi; Brami, Daniel; Zeigler-Allen, Lisa; Hoffman, Jeff; Goll, Johannes B; Fadrosh, Douglas; Glass, John; Adams, Mark D; Friedman, Robert; Venter, J Craig

2013-01-01

Understanding the microbial content of the air has important scientific, health, and economic implications. While studies have primarily characterized the taxonomic content of air samples by sequencing the 16S or 18S ribosomal RNA gene, direct analysis of the genomic content of airborne microorganisms has not been possible due to the extremely low density of biological material in airborne environments. We developed sampling and amplification methods to enable adequate DNA recovery to allow metagenomic profiling of air samples collected from indoor and outdoor environments. Air samples were collected from a large urban building, a medical center, a house, and a pier. Analyses of metagenomic data generated from these samples reveal airborne communities with a high degree of diversity and different genera abundance profiles. The identities of many of the taxonomic groups and protein families also allows for the identification of the likely sources of the sampled airborne bacteria.

A Metagenomic Framework for the Study of Airborne Microbial Communities

PubMed Central

Tenney, Aaron; McQuaid, Jeff; Williamson, Shannon; Thiagarajan, Mathangi; Brami, Daniel; Zeigler-Allen, Lisa; Hoffman, Jeff; Goll, Johannes B.; Fadrosh, Douglas; Glass, John; Adams, Mark D.; Friedman, Robert; Venter, J. Craig

2013-01-01

Understanding the microbial content of the air has important scientific, health, and economic implications. While studies have primarily characterized the taxonomic content of air samples by sequencing the 16S or 18S ribosomal RNA gene, direct analysis of the genomic content of airborne microorganisms has not been possible due to the extremely low density of biological material in airborne environments. We developed sampling and amplification methods to enable adequate DNA recovery to allow metagenomic profiling of air samples collected from indoor and outdoor environments. Air samples were collected from a large urban building, a medical center, a house, and a pier. Analyses of metagenomic data generated from these samples reveal airborne communities with a high degree of diversity and different genera abundance profiles. The identities of many of the taxonomic groups and protein families also allows for the identification of the likely sources of the sampled airborne bacteria. PMID:24349140

Technology perspectives in the future exploration of extreme environments

NASA Astrophysics Data System (ADS)

Cutts, J.; Balint, T.; Kolawa, El.; Peterson, C.

2007-08-01

Solar System exploration is driven by high priority science goals and objectives at diverse destinations, as described in the NRC Decadal Survey and in NASA's 2006 Solar System Exploration (SSE) Roadmap. Proposed missions to these targets encounter extreme environments, including high or low temperatures, high pressure, corrosion, high heat flux, radiation and thermal cycling. These conditions are often coupled, such as low temperature and high radiation at Europa; and high temperature and high pressure near the surface of Venus. Mitigation of these environmental conditions frequently reaches beyond technologies developed for terrestrial applications, for example, by the automotive and oil industries. Therefore, space agencies require dedicated technology developments to enable these future missions. Within NASA, proposed missions are divided into three categories. Competed small (Discovery class) and medium (New Frontiers class) missions are cost capped, thus limiting significant technology developments. Therefore, large (Flagship class) missions are required not only to tackle key science questions which can't be addressed by smaller missions, but also to develop mission enabling technologies that can feed forward to smaller missions as well. In a newly completed extreme environment technology assessment at NASA, we evaluated technologies from the current State of Practice (SoP) to advanced concepts for proposed missions over the next decades. Highlights of this report are discussed here, including systems architectures, such as hybrid systems; protection systems; high temperature electronics; power generation and storage; mobility technologies; sample acquisition and mechanisms; and the need to test these technologies in relevant environments. It is expected that the findings - documented in detail in NASA's Extreme Environments Technologies report - would help identifying future technology investment areas, and in turn enable or enhance planned SSE missions, while reducing mission cost and risk.

MSL's Widgets: Adding Rebustness to Martian Sample Acquisition, Handling, and Processing

NASA Technical Reports Server (NTRS)

Roumeliotis, Chris; Kennedy, Brett; Lin, Justin; DeGrosse, Patrick; Cady, Ian; Onufer, Nicholas; Sigel, Deborah; Jandura, Louise; Anderson, Robert; Katz, Ira;

Heatshield for Extreme Entry Environment Technology (HEEET)

NASA Technical Reports Server (NTRS)

Venkatapathy, E.; Ellerby, D.; Stackpoole, M..; Peterson, K.; Gage, P.; Beerman, A.; Blosser, M.; Chinnapongse, R.; Dillman, R.; Feldman, J.;

Extreme Environments Rig

NASA Image and Video Library

2013-08-13

The Glenn Extreme Environment Chamber (GEER) simulates the extreme conditions found in space and tests many devices that will explore Venus to see if they can withstand the punishing environment and temperatures over 800 degrees F.

Nontuberculous mycobacteria in the environment of Hranice Abyss, the world's deepest flooded cave (Hranice karst, Czech Republic).

PubMed

Pavlik, Ivo; Gersl, Milan; Bartos, Milan; Ulmann, Vit; Kaucka, Petra; Caha, Jan; Unc, Adrian; Hubelova, Dana; Konecny, Ondrej; Modra, Helena

2018-06-06

Nontuberculous mycobacteria (NTM) are widely distributed in the environment. On one hand, they are opportunistic pathogens for humans and animals, and on the other hand, they are effective in biodegradation of some persistent pollutants. Following the recently recorded large abundance of NTM in extreme geothermal environments, the aim of the study was to ascertain the occurrence of NTM in the extreme environment of the water zone of the Hranice Abyss (HA). The HA mineral water is acidic, with large concentrations of free CO 2 , and bacterial slimes creating characteristic mucilaginous formations. Both culture and molecular methods were used to compare the mycobacterial diversity across the linked but distinct ecosystems of HA and the adjacent Zbrašov Aragonite Caves (ZAC) with consideration of their pathogenic relevance. Six slowly growing NTM species (M. arupense, M. avium, M. florentinum, M. gordonae, M. intracellulare) and two rapidly growing NTM species (M. mucogenicum, M. sediminis) were identified in the water and in the dry zones at both sites. Proteobacteria were dominant in all the samples from both the HA and the ZAC. The bacterial microbiomes of the HA mineral water and HA slime were similar, but both differed from the microbiome in the ZAC mineral water. Actinobacteria, a phylum containing mycobacteria, was identified in all the samples at low proportional abundance. The majority of the detected NTM species belong among environmental opportunistic pathogens.

Thermal Evaluation of Fiber Bragg Gratings at Extreme Temperatures

NASA Technical Reports Server (NTRS)

Juergens, Jeffrey; Adamovsky, Grigory; Bhatt, Ramakrishna; Morscher, Gregory; Floyd, Bertram

2005-01-01

The development of integrated fiber optic sensors for use in aerospace health monitoring systems demands that the sensors be able to perform in extreme environments. In order to use fiber optic sensors effectively in an extreme environment one must have a thorough understanding of the sensor's capabilities, limitations, and performance under extreme environmental conditions. This paper reports on our current sensor evaluation examining the performance of freestanding fiber Bragg gratings (FBG) at extreme temperatures. While the ability of FBGs to survive at extreme temperatures has been established, their performance and long term survivability is not well documented. At extreme temperatures the grating structure would be expected to dissipate, degrading the sensors performance and eventually ceasing to return a detectable signal. The fiber jacket will dissipate leaving a brittle, unprotected fiber. For FBGs to be used in aerospace systems their performance and limitations need to be thoroughly understood at extreme temperatures. As the limits of the FBGs performance are pushed the long term survivability and performance of the sensor comes into question. We will not only examine the ability of FBGs to survive extreme temperatures but also look at their performance during many thermal cycles. This paper reports on test results of the performance of thermal cycling commercially available FBGs, at temperatures up to 1000 C, seen in aerospace applications. Additionally this paper will report on the performance of commercially available FBGs held at 1000 C for hundreds of hours. Throughout the evaluation process, various parameters of the FBGs performance were monitored and recorded. Several test samples were subjected to identical test conditions to allow for statistical analysis of the data. Test procedures, calibrations, referencing techniques, performance data, and interpretations and explanations of results are presented in the paper along with directions for future research.

Clean access, measurement, and sampling of Ellsworth Subglacial Lake: A method for exploring deep Antarctic subglacial lake environments

NASA Astrophysics Data System (ADS)

Siegert, Martin J.; Clarke, Rachel J.; Mowlem, Matt; Ross, Neil; Hill, Christopher S.; Tait, Andrew; Hodgson, Dominic; Parnell, John; Tranter, Martyn; Pearce, David; Bentley, Michael J.; Cockell, Charles; Tsaloglou, Maria-Nefeli; Smith, Andy; Woodward, John; Brito, Mario P.; Waugh, Ed

2012-01-01

Antarctic subglacial lakes are thought to be extreme habitats for microbial life and may contain important records of ice sheet history and climate change within their lake floor sediments. To find whether or not this is true, and to answer the science questions that would follow, direct measurement and sampling of these environments are required. Ever since the water depth of Vostok Subglacial Lake was shown to be >500 m, attention has been given to how these unique, ancient, and pristine environments may be entered without contamination and adverse disturbance. Several organizations have offered guidelines on the desirable cleanliness and sterility requirements for direct sampling experiments, including the U.S. National Academy of Sciences and the Scientific Committee on Antarctic Research. Here we summarize the scientific protocols and methods being developed for the exploration of Ellsworth Subglacial Lake in West Antarctica, planned for 2012-2013, which we offer as a guide to future subglacial environment research missions. The proposed exploration involves accessing the lake using a hot-water drill and deploying a sampling probe and sediment corer to allow sample collection. We focus here on how this can be undertaken with minimal environmental impact while maximizing scientific return without compromising the environment for future experiments.

The opposite end of the attention deficit hyperactivity disorder continuum: genetic and environmental aetiologies of extremely low ADHD traits.

PubMed

Greven, Corina U; Merwood, Andrew; van der Meer, Jolanda M J; Haworth, Claire M A; Rommelse, Nanda; Buitelaar, Jan K

2016-04-01

Although attention deficit hyperactivity disorder (ADHD) is thought to reflect a continuously distributed quantitative trait, it is assessed through binary diagnosis or skewed measures biased towards its high, symptomatic extreme. A growing trend is to study the positive tail of normally distributed traits, a promising avenue, for example, to study high intelligence to increase power for gene-hunting for intelligence. However, the emergence of such a 'positive genetics' model has been tempered for ADHD due to poor phenotypic resolution at the low extreme. Overcoming this methodological limitation, we conduct the first study to assess the aetiologies of low extreme ADHD traits. In a population-representative sample of 2,143 twins, the Strength and Weaknesses of ADHD Symptoms and Normal behaviour (SWAN) questionnaire was used to assess ADHD traits on a continuum from low to high. Aetiological influences on extreme ADHD traits were estimated using DeFries-Fulker extremes analysis. ADHD traits were related to behavioural, cognitive and home environmental outcomes using regression. Low extreme ADHD traits were significantly influenced by shared environmental factors (23-35%) but were not significantly heritable. In contrast, high-extreme ADHD traits showed significant heritability (39-51%) but no shared environmental influences. Compared to individuals with high extreme or with average levels of ADHD traits, individuals with low extreme ADHD traits showed fewer internalizing and externalizing behaviour problems, better cognitive performance and more positive behaviours and positive home environmental outcomes. Shared environmental influences on low extreme ADHD traits may reflect passive gene-environment correlation, which arises because parents provide environments as well as passing on genes. Studying the low extreme opens new avenues to study mechanisms underlying previously neglected positive behaviours. This is different from the current deficit-based model of intervention, but congruent with a population-level approach to improving youth wellbeing. © 2015 The Authors. Journal of Child Psychology and Psychiatry published by John Wiley & Sons Ltd on behalf of Association for Child and Adolescent Mental Health.

Progress Towards Providing Heat-Shield for Extreme Entry Environment Technology (HEEET) for Venus and Other New Froniters Missions

NASA Astrophysics Data System (ADS)

Venkatapathy, E.; Ellerby, D.; Gage, P.

2017-11-01

HEEET, in development since 2014 with the goal of enabling missions to Venus, Saturn and other high-speed sample return missions, is incentivized by SMD-PSD and will be delivered at TRL 6 by FY'18. This presentation will cover the current status.

Exploration of Deinococcus-Thermus molecular diversity by novel group-specific PCR primers

PubMed Central

Theodorakopoulos, Nicolas; Bachar, Dipankar; Christen, Richard; Alain, Karine; Chapon, Virginie

2013-01-01

The deeply branching Deinococcus-Thermus lineage is recognized as one of the most extremophilic phylum of bacteria. In previous studies, the presence of Deinococcus-related bacteria in the hot arid Tunisian desert of Tataouine was demonstrated through combined molecular and culture-based approaches. Similarly, Thermus-related bacteria have been detected in Tunisian geothermal springs. The present work was conducted to explore the molecular diversity within the Deinococcus-Thermus phylum in these extreme environments. A set of specific primers was designed in silico on the basis of 16S rRNA gene sequences, validated for the specific detection of reference strains, and used for the polymerase chain reaction (PCR) amplification of metagenomic DNA retrieved from the Tataouine desert sand and Tunisian hot spring water samples. These analyses have revealed the presence of previously undescribed Deinococcus-Thermus bacterial sequences within these extreme environments. The primers designed in this study thus represent a powerful tool for the rapid detection of Deinococcus-Thermus in environmental samples and could also be applicable to clarify the biogeography of the Deinococcus-Thermus phylum. PMID:23996915

High Temperature Piezoelectric Drill

NASA Technical Reports Server (NTRS)

Bao, Xiaoqi; Scott, James; Boudreau, Kate; Bar-Cohen, Yoseph; Sherrit, Stewart; Badescu, Mircea; Shrout, Tom; Zhang, Shujun

2009-01-01

The current NASA Decadal mission planning effort has identified Venus as a significant scientific target for a surface in-situ sampling/analyzing mission. The Venus environment represents several extremes including high temperature (460 deg C), high pressure (9 MPa), and potentially corrosive (condensed sulfuric acid droplets that adhere to surfaces during entry) environments. This technology challenge requires new rock sampling tools for these extreme conditions. Piezoelectric materials can potentially operate over a wide temperature range. Single crystals, like LiNbO3, have a Curie temperature that is higher than 1000 deg C and the piezoelectric ceramics Bismuth Titanate higher than 600 deg C. A study of the feasibility of producing piezoelectric drills that can operate in the temperature range up to 500 deg C was conducted. The study includes the high temperature properties investigations of engineering materials and piezoelectric ceramics with different formulas and doping. The drilling performances of a prototype Ultrasonic/Sonic Drill/Corer (USDC) using high temperate piezoelectric ceramics and single crystal were tested at temperature up to 500 deg C. The detailed results of our study and a discussion of the future work on performance improvements are presented in this paper.

Exploring microbial diversity in volcanic environments: a review of methods in DNA extraction.

PubMed

Herrera, Aude; Cockell, Charles S

2007-07-01

The last decade has been marked by a large number of studies focused on understanding the distribution of microorganisms in volcanic environments. These studies are motivated by the desire to elucidate how the geochemically extreme conditions of such environments can influence microbial diversity both on the surface and in the subsurface of the Earth. The exploration of microbial community diversity has generally not relied on culture-dependent methods, but has been carried out using environmental DNA extraction. Because of the large diversity of chemically and physically complex samples, extracting DNA from volcanic environments is technically challenging. In view of the emerging literature, and our own experience in the optimisation of methods for DNA extraction from volcanic materials, it is timely to provide a methodological comparison. This review highlights and discusses new insights and methods published on DNA extraction methods from volcanic samples, considering the different volcanic environments. A description of a recent method for DNA extraction from basalt and obsidian glass rock samples from Iceland is included. Finally, we discuss these approaches in the wider context of modern work to understand the microbial diversity of volcanic environments.

Glenn Extreme Environment Rig (GEER)

NASA Image and Video Library

2017-01-17

NASA Glenn research engineers prepare our extreme environments chamber (GEER) for a test. GEER, which simulates the extreme conditions found in space, tests many devices that will explore Venus to see if they can withstand the punishing environment and temperatures over 800˚F.

Ellipsometric Analysis of Contaminant Layer on Optical Witness Samples from MISSE

NASA Technical Reports Server (NTRS)

Norwood, Joseph K.

2007-01-01

Several optical witness samples included in the Materials for International Space Station Experiment (MISSE) trays have been analyzed with a variable angle spectroscopic ellipsometer or VASE. Witness samples of gold or platinum mirrors are extremely useful as collectors of space-borne contamination, due to the relative inertness of these noble metals in the atomic oxygen-rich environment of LEO. Highly accurate thickness measurements, typically at the sub-nanometer scale, may be achieved with this method, which uses polarized light in a spectral range of 300 to 1300 nanometers at several angles of incidence to the sample surface.

Fiberoptic characteristics for extreme operating environments

NASA Technical Reports Server (NTRS)

Delcher, R. C.

1992-01-01

Fiberoptics could offer several major benefits for cryogenic liquid-fueled rocket engines, including lightning immunity, weight reduction, and the possibility of implementing a number of new measurements for engine condition monitoring. The technical feasibility of using fiberoptics in the severe environments posed by cryogenic liquid-fueled rocket engines was determined. The issues of importance and subsequent requirements for this use of fiberoptics were compiled. These included temperature ranges, moisture embrittlement succeptability, and the ability to withstand extreme shock and vibration levels. Different types of optical fibers were evaluated and several types of optical fibers' ability to withstand use in cryogenic liquid-fueled rocket engines was demonstrated through environmental testing of samples. This testing included: cold-bend testing, moisture embrittlement testing, temperature cycling, temperature extremes testing, vibration testing, and shock testing. Three of five fiber samples withstood the tests to a level proving feasibility, and two of these remained intact in all six of the tests. A fiberoptic bundle was also tested, and completed testing without breakage. Preliminary cabling and harnessing for fiber protection was also demonstrated. According to cable manufacturers, the successful -300 F cold bend, vibration, and shock tests are the first instance of any major fiberoptic cable testing below roughly -55 F. This program has demonstrated the basic technical feasibility of implementing optical fibers on cryogenic liquid-fueled rocket engines, and a development plan is included highlighting requirements and issues for such an implementation.

Plant volatiles in extreme terrestrial and marine environments.

PubMed

Rinnan, Riikka; Steinke, Michael; McGenity, Terry; Loreto, Francesco

2014-08-01

This review summarizes the current understanding on plant and algal volatile organic compound (VOC) production and emission in extreme environments, where temperature, water availability, salinity or other environmental factors pose stress on vegetation. Here, the extreme environments include terrestrial systems, such as arctic tundra, deserts, CO₂ springs and wetlands, and marine systems such as sea ice, tidal rock pools and hypersaline environments, with mangroves and salt marshes at the land-sea interface. The emission potentials at fixed temperature and light level or actual emission rates for phototrophs in extreme environments are frequently higher than for organisms from less stressful environments. For example, plants from the arctic tundra appear to have higher emission potentials for isoprenoids than temperate species, and hypersaline marine habitats contribute to global dimethyl sulphide (DMS) emissions in significant amounts. DMS emissions are more widespread than previously considered, for example, in salt marshes and some desert plants. The reason for widespread VOC, especially isoprenoid, emissions from different extreme environments deserves further attention, as these compounds may have important roles in stress resistance and adaptation to extremes. Climate warming is likely to significantly increase VOC emissions from extreme environments both by direct effects on VOC production and volatility, and indirectly by altering the composition of the vegetation. © 2014 John Wiley & Sons Ltd.

Peas in a Pod: Environment and Ionization in Green Pea Galaxies

NASA Astrophysics Data System (ADS)

Kurtz, Heather; Jaskot, Anne; Drew, Patrick; Pare, Dylan; Griffin, Jon; Petersen, Michael

2016-01-01

The Green Peas are extreme, highly ionized, starburst galaxies with strong [OIII] 5007 emission. Using the Sloan Digital Sky Survey, we present statistics on the environment of Green Peas and investigate its effects on their ionized gas properties. Although most dwarf starburst galaxies are in low-density environments, we identify a sample of Green Peas in dense environments. Emission line observations with the WIYN 0.9-meter telescope at Kitt Peak reveal that one cluster Green Pea is more highly ionized in the direction of the cluster center. Ram pressure stripping likely generates this ionization gradient. We explore the role of the environment in enhancing star formation rates and ionization, and we compare the nebular properties of Green Peas in high-density environments to those in low-density environments.

Phospholipid and Respiratory Quinone Analyses From Extreme Environments

NASA Astrophysics Data System (ADS)

Pfiffner, S. M.

2008-12-01

Extreme environments on Earth have been chosen as surrogate sites to test methods and strategies for the deployment of space craft in the search for extraterrestrial life. Surrogate sites for many of the NASA astrobiology institutes include the South African gold mines, Canadian subpermafrost, Atacama Desert, and acid rock drainage. Soils, sediments, rock cores, fracture waters, biofilms, and service and drill waters represent the types of samples collected from these sites. These samples were analyzed by gas chromatography mass spectrometry for phospholipid fatty acid methyl esters and by high performance liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry for respiratory quinones. Phospholipid analyses provided estimates of biomass, community composition, and compositional changes related to nutritional limitations or exposure to toxic conditions. Similar to phospholipid analyses, respiratory quinone analyses afforded identification of certain types of microorganisms in the community based on respiration and offered clues to in situ redox conditions. Depending on the number of samples analyzed, selected multivariate statistical methods were applied to relate membrane lipid results with site biogeochemical parameters. Successful detection of life signatures and refinement of methodologies at surrogate sites on Earth will be critical for the recognition of extraterrestrial life. At this time, membrane lipid analyses provide useful information not easily obtained by other molecular techniques.

The identification of plankton tropical status in the Wonokromo, Dadapan and Juanda extreme water estuary

NASA Astrophysics Data System (ADS)

Sari, L. A.; Satyantini, W. H.; Manan, A.; Pursetyo, K. T.; Dewi, N. N.

2018-04-01

Wonokromo, Dadapan and Juanda estuaries are extreme waters located around Surabaya environment. This is because of a lot of organic material intake, which provided nutrients for plankton growth. In addition, the waters is also dynamic in reason of physico-chemical, geological and biological processes controlled by the tides and freshwater run-off from the river that empties into it. The objective of this study was to identify the presentation of plankton in extreme waters based on brightness and ammonia level. The study was conducted in January 2017. Three sampling locations were Wonokromo, Dadapan and Juanda estuaries. Each station consists of three points based on distances, which were 400, 700, and 1000 meters from the coastline. The brightness in Wonokromo, Dadapan, and Juanda environment was 60, 40, and 100 cm, respectively. The result of ammonia in Wonokromo, Dadapan, and Juanda estuary was 0.837, 0.626, and 0.396 mg/L, correspondingly. Nine classes of phytoplankton’s were found in three locations (bacillariophyceae, dynophyceae, chlorophyceae, cyanophyceae, crysophyceae, euglenoidea, trebouxlophyceae, mediophyceae, and nitachiaceae) and five classes of zooplanktons (maxillopoda, hexanuplia, copepoda, malacostraca, and oligotrichea). The density of plankton in Wonokromo, Dadapan and Juanda environments, was 37.64, 63.80, and 352.85 cells/L, respectively.

Microbial diversity of extreme habitats in human homes.

PubMed

Savage, Amy M; Hills, Justin; Driscoll, Katherine; Fergus, Daniel J; Grunden, Amy M; Dunn, Robert R

2016-01-01

High-throughput sequencing techniques have opened up the world of microbial diversity to scientists, and a flurry of studies in the most remote and extreme habitats on earth have begun to elucidate the key roles of microbes in ecosystems with extreme conditions. These same environmental extremes can also be found closer to humans, even in our homes. Here, we used high-throughput sequencing techniques to assess bacterial and archaeal diversity in the extreme environments inside human homes (e.g., dishwashers, hot water heaters, washing machine bleach reservoirs, etc.). We focused on habitats in the home with extreme temperature, pH, and chemical environmental conditions. We found a lower diversity of microbes in these extreme home environments compared to less extreme habitats in the home. However, we were nonetheless able to detect sequences from a relatively diverse array of bacteria and archaea. Habitats with extreme temperatures alone appeared to be able to support a greater diversity of microbes than habitats with extreme pH or extreme chemical environments alone. Microbial diversity was lowest when habitats had both extreme temperature and one of these other extremes. In habitats with both extreme temperatures and extreme pH, taxa with known associations with extreme conditions dominated. Our findings highlight the importance of examining interactive effects of multiple environmental extremes on microbial communities. Inasmuch as taxa from extreme environments can be both beneficial and harmful to humans, our findings also suggest future work to understand both the threats and opportunities posed by the life in these habitats.

Bacterial community initial development in proglacial soils of Larsemann hill, East Antarctica

NASA Astrophysics Data System (ADS)

Ma, H.; Yan, W.; Shi, G.; Sun, B.; Zhang, Y.; Xiao, X.

2016-12-01

Glacial forefields are considered ideal places to explore how microbial communities will response to climate-driven environmental changes. Our knowledge of how the bacterial community activities and structure was influenced by changing environment due to glacier retreat is still very limited, especially at the initial stage of glacier retreat. The short gradient soil samples including the ice free and ice covered sites were sampled in the forehead of East Antarctica ice sheet, in Larsemann Hills. By employing the Miseq sequencing methods, 1.8 x104 high-quality sequences were gotten for each sample and the bacterial diversity including abundant bacteria and rare bacteria were studied and compared between the gradient samples. Even though in such an extreme stress condition, the bacterial diversity was high. The coefficient of variance between the five sites of abundant group was 0.886 which was higher than that of the top 20 rare group (0.159) significantly (unpaired t test, p-value<0.0001) suggesting that the abundant bacterial communities were more sensitive to the ice sheet change in the initial stage than rare bacteria did. And the abundant bacteria contributed the community structure more than the rare bacteria did. The rare group acted more like seed bank to keep the community functionality in the forehead of sheet. And the ice thickness was the major factor to affect the abundant bacterial community. Given the fact that Antarctica environment was more sensitive to the global warming, the study about abundant and rare bacteria response to condition change will be helpful to precisely predict community response to climate change in polar region. This finding will improve the understanding about the relationship between community structure and environment condition in extreme stress condition.

Extreme Environments: The Ghetto and the South Pole.

ERIC Educational Resources Information Center

Pierce, Chester M.

Extreme environments, such as polar regions or space crafts, provide an analogue for speculations concerning the needs of, educational provisions for, and environmental impacts on ghetto youth in kindergarten through the third grade. This discussion first centers on the common qualities of an extreme environment (whether exotic or mundane): forced…

A Neuroscience Approach to Optimizing Brain Resources for Human Performance in Extreme Environments

PubMed Central

Paulus, Martin P.; Potterat, Eric G.; Taylor, Marcus K.; Van Orden, Karl F.; Bauman, James; Momen, Nausheen; Padilla, Genieleah A.; Swain, Judith L.

2009-01-01

Extreme environments requiring optimal cognitive and behavioral performance occur in a wide variety of situations ranging from complex combat operations to elite athletic competitions. Although a large literature characterizes psychological and other aspects of individual differences in performances in extreme environments, virtually nothing is known about the underlying neural basis for these differences. This review summarizes the cognitive, emotional, and behavioral consequences of exposure to extreme environments, discusses predictors of performance, and builds a case for the use of neuroscience approaches to quantify and understand optimal cognitive and behavioral performance. Extreme environments are defined as an external context that exposes individuals to demanding psychological and/or physical conditions, and which may have profound effects on cognitive and behavioral performance. Examples of these types of environments include combat situations, Olympic-level competition, and expeditions in extreme cold, at high altitudes, or in space. Optimal performance is defined as the degree to which individuals achieve a desired outcome when completing goal-oriented tasks. It is hypothesized that individual variability with respect to optimal performance in extreme environments depends on a well “contextualized” internal body state that is associated with an appropriate potential to act. This hypothesis can be translated into an experimental approach that may be useful for quantifying the degree to which individuals are particularly suited to performing optimally in demanding environments. PMID:19447132

Specialization of Bacillus in the Geochemically Challenged Environment of Death Valley

NASA Astrophysics Data System (ADS)

Kopac, S.

2014-04-01

Death Valley is the hottest, driest place in North America, a desert with soils containing toxic elements such as boron and lead. While most organisms are unable to survive under these conditions, a diverse community of bacteria survives here. What has enabled bacteria to adapt and thrive in a plethora of extreme and stressful environments where other organisms are unable to grow? The unique environmental adaptations that distinguish ecologically distinct bacterial groups (ecotypes) remain a mystery, in contrast to many animal species (perhaps most notably Darwin's ecologically distinct finch species). We resolve the ecological factors associated with recently diverged ecotypes of the soil bacteria Bacillus subtilis and Bacillus licheniformis, isolated from the dry, geochemically challenging soils of Death Valley, CA. To investigate speciation associated with challenging environmental parameters, we sampled soil transects along a 400m stretch that parallels a decrease in salinity adjacent to a salt flat; transects also encompass gradients in soil B, Cu, Fe, NO3, and P, all of which were quantified in our soil samples. We demarcated strains using Ecotype Simulation, a sequence-based algorithm. Each ecotype's habitat associations were determined with respect to salinity, B, Cu, Fe, NO3, and P. In addition, our sample strains were tested for tolerance of copper, boron and salinity (all known to inhibit growth at high concentrations) by comparing their growth over a 20 hour period. Ecotypes differed in their habitat associations with salinity, boron, copper, iron, and other ecological factors; these environmental dimensions are likely causing speciation of B. subtilis-licheniformis ecotypes at our sample site. Strains also differed in tolerance of boron and copper, providing evidence that our sequence-based demarcations reflect real differences in metabolism. By better understanding the relationship between bacterial speciation and the environment, we can begin to predict the habitability of unexplored extreme and extra-Earth environments.

Capillary Electrophoresis Analysis of Organic Amines and Amino Acids in Saline and Acidic Samples Using the Mars Organic Analyzer

NASA Astrophysics Data System (ADS)

Stockton, Amanda M.; Chiesl, Thomas N.; Lowenstein, Tim K.; Amashukeli, Xenia; Grunthaner, Frank; Mathies, Richard A.

2009-11-01

The Mars Organic Analyzer (MOA) has enabled the sensitive detection of amino acid and amine biomarkers in laboratory standards and in a variety of field sample tests. However, the MOA is challenged when samples are extremely acidic and saline or contain polyvalent cations. Here, we have optimized the MOA analysis, sample labeling, and sample dilution buffers to handle such challenging samples more robustly. Higher ionic strength buffer systems with pKa values near pH 9 were developed to provide better buffering capacity and salt tolerance. The addition of ethylaminediaminetetraacetic acid (EDTA) ameliorates the negative effects of multivalent cations. The optimized protocol utilizes a 75 mM borate buffer (pH 9.5) for Pacific Blue labeling of amines and amino acids. After labeling, 50 mM (final concentration) EDTA is added to samples containing divalent cations to ameliorate their effects. This optimized protocol was used to successfully analyze amino acids in a saturated brine sample from Saline Valley, California, and a subcritical water extract of a highly acidic sample from the Río Tinto, Spain. This work expands the analytical capabilities of the MOA and increases its sensitivity and robustness for samples from extraterrestrial environments that may exhibit pH and salt extremes as well as metal ions.

Capillary electrophoresis analysis of organic amines and amino acids in saline and acidic samples using the Mars organic analyzer.

PubMed

Stockton, Amanda M; Chiesl, Thomas N; Lowenstein, Tim K; Amashukeli, Xenia; Grunthaner, Frank; Mathies, Richard A

2009-11-01

The Mars Organic Analyzer (MOA) has enabled the sensitive detection of amino acid and amine biomarkers in laboratory standards and in a variety of field sample tests. However, the MOA is challenged when samples are extremely acidic and saline or contain polyvalent cations. Here, we have optimized the MOA analysis, sample labeling, and sample dilution buffers to handle such challenging samples more robustly. Higher ionic strength buffer systems with pK(a) values near pH 9 were developed to provide better buffering capacity and salt tolerance. The addition of ethylaminediaminetetraacetic acid (EDTA) ameliorates the negative effects of multivalent cations. The optimized protocol utilizes a 75 mM borate buffer (pH 9.5) for Pacific Blue labeling of amines and amino acids. After labeling, 50 mM (final concentration) EDTA is added to samples containing divalent cations to ameliorate their effects. This optimized protocol was used to successfully analyze amino acids in a saturated brine sample from Saline Valley, California, and a subcritical water extract of a highly acidic sample from the Río Tinto, Spain. This work expands the analytical capabilities of the MOA and increases its sensitivity and robustness for samples from extraterrestrial environments that may exhibit pH and salt extremes as well as metal ions.

Snapshot Survey of the Globular Cluster Populations of Isolated Early Type Galaxies

NASA Astrophysics Data System (ADS)

Gregg, Michael

2017-08-01

We propose WFC3/UVIS snapshot observations of a sample of 75 isolated early type galaxiesresiding in cosmic voids or extremely low density regions. The primary aim is to usetheir globular cluster populations to reconstruct their evolutionary history, revealingif, how, and why void ellipticals differ from cluster ellipticals. The galaxies span arange of luminosities, providing a varied sample for comparison with the well-documentedglobular cluster populations in denser environments. This proposed WFC3 study of isolatedearly type galaxies breaks new ground by targeting a sample which has thus far receivedlittle attention, and, significantly, this will be the first such study with HST.Characterizing early type galaxies in voids and their GC systems promises to increase ourunderstanding of galaxy formation and evolution of galaxies in general because isolatedobjects are the best approximation to a control sample that we have for understanding theinfluence of environment on formation and evolution. Whether these isolated objects turnout to be identical to or distinct from counterparts in other regions of the Universe,they will supply insight into the formation and evolution of all galaxies. Parallel ACSimaging will help to characterize the near field environments of the sample.

Self-Recovery Experiments in Extreme Environments Using a Field Programmable Transistor Array

NASA Technical Reports Server (NTRS)

Stoica, Adrian; Keymeulen, Didier; Arslan, Tughrul; Duong, Vu; Zebulum, Ricardo; Ferguson, Ian; Guo, Xin

2004-01-01

Temperature and radiation tolerant electronics, as well as long life survivability are key capabilities required for future NASA missions. Current approaches to electronics for extreme environments focus on component level robustness and hardening. However, current technology can only ensure very limited lifetime in extreme environments. This paper describes novel experiments that allow adaptive in-situ circuit redesign/reconfiguration during operation in extreme temperature and radiation environments. This technology would complement material/device advancements and increase the mission capability to survive harsh environments. The approach is demonstrated on a mixed-signal programmable chip (FPTA-2), which recovers functionality for temperatures until 28 C and with total radiation dose up to 250kRad.

Extermophylic microorganisms: issue of interplanetary transfer on external spacecraft surfaces.

NASA Astrophysics Data System (ADS)

Novikova, N.; Deshevaya, E.; Polykarpov, N.; Svistunova, Y.; Grigoriev, A.

Interplanetary transfer of terrestrial microbes capable of surviving in extreme environments and planetary protection from accidental biocontamination by them are the issues of major practical rather than hypothetical value The natural resistance of microbes to extreme environments and a possibility of their transfer beyond geographical barriers of Earth on external spacecraft surfaces have brought forward a need in profound research into the likelihood of their survival in outer space Hardware and a program have been developed at the State Scientific Research Center of the Russian Federation -- Institute for Biomedical Problems with the goal of carrying out a space experiment Biorisk The experiment was aimed at assessing the possibility of long-term comparable with the duration of the Martian flight survival of microorganisms in outer space on materials used in space industry Samples of materials were contaminated with test cultures of bacteria Bacillus and fungi Aspergillus Penicillium Cladosporium known to be common residents of various environments on Earth and resistant to multiple alternation of high and low temperatures Materials used in the construction of external spacecraft surfaces such as steel aluminium alloy heat-insulating coating were chosen as test samples for the experiment Containers with materials and test microorganisms were placed on the external side of the Russian segment of the ISS Unique data have been accumulated after a 204 day exposure on the external side of the ISS which have proved that

Diversity of extremophilic bacteria in the sediment of high-altitude lakes located in the mountain desert of Ojos del Salado volcano, Dry-Andes.

PubMed

Aszalós, Júlia Margit; Krett, Gergely; Anda, Dóra; Márialigeti, Károly; Nagy, Balázs; Borsodi, Andrea K

2016-09-01

Ojos del Salado, the highest volcano on Earth is surrounded by a special mountain desert with extreme aridity, great daily temperature range, intense solar radiation, and permafrost from 5000 meters above sea level. Several saline lakes and permafrost derived high-altitude lakes can be found in this area, often surrounded by fumaroles and hot springs. The aim of this study was to gain information about the bacterial communities inhabiting the sediment of high-altitude lakes of the Ojos del Salado region located between 3770 and 6500 m. Altogether 11 sediment samples from 4 different altitudes were examined with 16S rRNA gene based denaturing gradient gel electrophoresis and clone libraries. Members of 17 phyla or candidate divisions were detected with the dominance of Proteobacteria, Acidobacteria, Actinobacteria and Bacteroidetes. The bacterial community composition was determined mainly by the altitude of the sampling sites; nevertheless, the extreme aridity and the active volcanism had a strong influence on it. Most of the sequences showed the highest relation to bacterial species or uncultured clones from similar extreme environments.

Geophysical, geochemical, mineralogical, and enivronmental data for rock samples collected in a mineralized volcanic environment, upper Animas River watershed, Colorado

USGS Publications Warehouse

McCafferty, A.E.; Horton, R.J.; Stanton, M.R.; McDougal, R.R.; Fey, D.L.

2011-01-01

* provide measurements to study the geochemical, mineralogical, and geophysical characteristics of rocks having weak to extreme degrees of alteration and to develop an understanding of how these characteristics change with alteration type. Data are provided in two digital formats: an Arc/Info geodatabase and a Microsoft Excel spreadsheet.

Bacterial methane oxidation in sea-floor gas hydrate: Significance to life in extreme environments

NASA Astrophysics Data System (ADS)

Sassen, Roger; MacDonald, Ian R.; Guinasso, Norman L., Jr.; Joye, Samantha; Requejo, Adolfo G.; Sweet, Stephen T.; Alcalá-Herrera, Javier; Defreitas, Debra A.; Schink, David R.

1998-09-01

Samples of thermogenic hydrocarbon gases, from vents and gas hydrate mounds within a sea-floor chemosynthetic community on the Gulf of Mexico continental slope at about 540 m depth, were collected by research submersible. Our study area is characterized by low water temperature (mean =7 °C), high pressure (about 5400 kPa), and abundant structure II gas hydrate. Bacterial oxidation of hydrate-bound methane (CH4) is indicated by three isotopic properties of gas hydrate samples. Relative to the vent gas from which the gas hydrate formed, (1) methane-bound methane is enriched in 13C by as much as 3.8‰ PDB (Peedee belemnite), (2) hydrate-bound methane is enriched in deuterium (D) by as much as 37‰ SMOW (standard mean ocean water), and (3) hydrate-bound carbon dioxide (CO2) is depleted in 13C by as much as 22.4‰ PDB. Hydrate-associated authigenic carbonate rock is also depleted in 13C. Bacterial oxidation of methane is a driving force in chemosynthetic communities, and in the concomitant precipitation of authigenic carbonate rock that modifies sea-floor geology. Bacterial oxidation of hydrate-bound methane expands the potential boundaries of life in extreme environments.

IDE spatio-temporal impact fluxes and high time-resolution studies of multi-impact events and long-lived debris clouds

NASA Technical Reports Server (NTRS)

Mulholland, J. Derral; Singer, S. Fred; Oliver, John P.; Weinberg, Jerry L.; Cooke, William J.; Montague, Nancy L.; Wortman, Jim J.; Kassel, Phillip C.; Kinard, William H.

1992-01-01

The purpose of the Interplanetary Dust Experiment (IDE) on the Long Duration Exposure Facility (LDEF) was to sample the cosmic dust environment and to use the spatio-temporal aspect of the experiment to distinguish between the various components of the environment: zodiacal cloud, beta meteoroids, meteor streams, interstellar dust, and orbital debris. It was found that the introduction of precise time and even rudimentary directionality as co-lateral observables in sampling the particulate environment in near-Earth space produces an enormous qualitative improvement in the information content of the impact data. The orbital debris population is extremely clumpy, being dominated by persistent clouds in which the fluxes may rise orders of magnitude above the background. The IDE data suggest a strategy to minimize the damage to sensitive spacecraft components, using the observed characteristics of cloud encounters.

Arcjet Testing of Micro-Meteoroid Impacted Thermal Protection Materials

NASA Technical Reports Server (NTRS)

Agrawal, Parul; Munk, Michelle M.; Glaab, Louis J.

2013-01-01

There are several harsh space environments that could affect thermal protection systems and in turn pose risks to the atmospheric entry vehicles. These environments include micrometeoroid impact, extreme cold temperatures, and ionizing radiation during deep space cruise, all followed by atmospheric entry heating. To mitigate these risks, different thermal protection material samples were subjected to multiple tests, including hyper velocity impact, cold soak, irradiation, and arcjet testing, at various NASA facilities that simulated these environments. The materials included a variety of honeycomb packed ablative materials as well as carbon-based non-ablative thermal protection systems. The present paper describes the results of the multiple test campaign with a focus on arcjet testing of thermal protection materials. The tests showed promising results for ablative materials. However, the carbon-based non-ablative system presented some concerns regarding the potential risks to an entry vehicle. This study provides valuable information regarding the capability of various thermal protection materials to withstand harsh space environments, which is critical to sample return and planetary entry missions.

Grain size analysis and depositional environment of shallow marine to basin floor, Kelantan River Delta

NASA Astrophysics Data System (ADS)

Afifah, M. R. Nurul; Aziz, A. Che; Roslan, M. Kamal

2015-09-01

Sediment samples were collected from the shallow marine from Kuala Besar, Kelantan outwards to the basin floor of South China Sea which consisted of quaternary bottom sediments. Sixty five samples were analysed for their grain size distribution and statistical relationships. Basic statistical analysis like mean, standard deviation, skewness and kurtosis were calculated and used to differentiate the depositional environment of the sediments and to derive the uniformity of depositional environment either from the beach or river environment. The sediments of all areas were varied in their sorting ranging from very well sorted to poorly sorted, strongly negative skewed to strongly positive skewed, and extremely leptokurtic to very platykurtic in nature. Bivariate plots between the grain-size parameters were then interpreted and the Coarsest-Median (CM) pattern showed the trend suggesting relationships between sediments influenced by three ongoing hydrodynamic factors namely turbidity current, littoral drift and waves dynamic, which functioned to control the sediments distribution pattern in various ways.

Trajectories of the home learning environment across the first 5 years: associations with children's vocabulary and literacy skills at prekindergarten.

PubMed

Rodriguez, Eileen T; Tamis-LeMonda, Catherine S

2011-01-01

Children's home learning environments were examined in a low-income sample of 1,852 children and families when children were 15, 25, 37, and 63 months. During home visits, children's participation in literacy activities, the quality of mothers' engagements with their children, and the availability of learning materials were assessed, yielding a total learning environment score at each age. At 63 months, children's vocabulary and literacy skills were assessed. Six learning environment trajectories were identified, including environments that were consistently low, environments that were consistently high, and environments characterized by varying patterns of change. The skills of children at the extremes of learning environment trajectories differed by more than 1 SD and the timing of learning experiences related to specific emerging skills. © 2011 The Authors. Child Development © 2011 Society for Research in Child Development, Inc.

Surface roughness control by extreme ultraviolet (EUV) radiation

NASA Astrophysics Data System (ADS)

Ahad, Inam Ul; Obeidi, Muhannad Ahmed; Budner, Bogusław; Bartnik, Andrzej; Fiedorowicz, Henryk; Brabazon, Dermot

2017-10-01

Surface roughness control of polymeric materials is often desirable in various biomedical engineering applications related to biocompatibility control, separation science and surface wettability control. In this study, Polyethylene terephthalate (PET) polymer films were irradiated with Extreme ultraviolet (EUV) photons in nitrogen environment and investigations were performed on surface roughness modification via EUV exposure. The samples were irradiated at 3 mm and 4 mm distance from the focal spot to investigate the effect of EUV fluence on topography. The topography of the EUV treated PET samples were studied by AFM. The detailed scanning was also performed on the sample irradiated at 3 mm. It was observed that the average surface roughness of PET samples was increased from 9 nm (pristine sample) to 280 nm and 253 nm for EUV irradiated samples. Detailed AFM studies confirmed the presence of 1.8 mm wide period U-shaped channels in EUV exposed PET samples. The walls of the channels were having FWHM of about 0.4 mm. The channels were created due to translatory movements of the sample in horizontal and transverse directions during the EUV exposure. The increased surface roughness is useful for many applications. The nanoscale channels fabricated by EUV exposure could be interesting for microfluidic applications based on lab-on-a-chip (LOC) devices.

Characterizing the Chemical Stability of High Temperature Materials for Application in Extreme Environments

NASA Technical Reports Server (NTRS)

Opila, Elizabeth

2005-01-01

The chemical stability of high temperature materials must be known for use in the extreme environments of combustion applications. The characterization techniques available at NASA Glenn Research Center vary from fundamental thermodynamic property determination to material durability testing in actual engine environments. In this paper some of the unique techniques and facilities available at NASA Glenn will be reviewed. Multiple cell Knudsen effusion mass spectrometry is used to determine thermodynamic data by sampling gas species formed by reaction or equilibration in a Knudsen cell held in a vacuum. The transpiration technique can also be used to determine thermodynamic data of volatile species but at atmospheric pressures. Thermodynamic data in the Si-O-H(g) system were determined with this technique. Free Jet Sampling Mass Spectrometry can be used to study gas-solid interactions at a pressure of one atmosphere. Volatile Si(OH)4(g) was identified by this mass spectrometry technique. A High Pressure Burner Rig is used to expose high temperature materials in hydrocarbon-fueled combustion environments. Silicon carbide (SiC) volatility rates were measured in the burner rig as a function of total pressure, gas velocity and temperature. Finally, the Research Combustion Lab Rocket Test Cell is used to expose high temperature materials in hydrogen/oxygen rocket engine environments to assess material durability. SiC recession due to rocket engine exposures was measured as a function of oxidant/fuel ratio, temperature, and total pressure. The emphasis of the discussion for all techniques will be placed on experimental factors that must be controlled for accurate acquisition of results and reliable prediction of high temperature material chemical stability.

Magnetic-Field-Assisted Assembly of Ordered Multifunctional Ceramic Nanocomposites for Extreme Environments

DTIC Science & Technology

2016-04-01

SUBJECT TERMS carbon nanotubes, composite, electromagnetic shielding , extreme environments, magnetism , fibers, woven composite, boron nitride...AFRL-AFOSR-VA-TR-2016-0158 Magnetic -Field-Assisted Assembly of Ordered Multifunctional Ceramic Nanocomposites for Extreme Environments Konstantin...From - To) 15 Sep 2012 to 14 Nov 2017 4. TITLE AND SUBTITLE Magnetic -Field-Assisted Assembly of Ordered Multifunctional Ceramic Nanocomposites for

Thermoelectric technique to precisely control hyperthermic exposures of human whole blood.

PubMed

DuBose, D A; Langevin, R C; Morehouse, D H

1996-12-01

The need in military research to avoid exposing humans to harsh environments and reduce animal use requires the development of in vitro models for the study of hyperthermic injury. A thermoelectric module (TEM) system was employed to heat human whole blood (HWB) in a manner similar to that experienced by heat-stroked rats. This system precisely and accurately replicated mild, moderate, and extreme heat-stress exposures. Temperature changes could be monitored without the introduction of a test sample thermistor, which reduced contamination problems. HWB with hematocrits of 45 or 50% had similar heating curves, indicating that the system compensated for differences in sample character. The unit's size permitted its containment within a standard carbon dioxide incubator to further control sample environment. These results indicate that the TEM system can precisely control temperature change in this heat stress in vitro model employing HWB. Information obtained from such a model could contribute to military preparedness.

Meiofaunal Richness in Highly Acidic Hot Springs in Unzen-Amakusa National Park, Japan, Including the First Rediscovery Attempt for Mesotardigrada.

PubMed

Suzuki, Atsushi C; Kagoshima, Hiroshi; Chilton, Glen; Grothman, Gary T; Johansson, Carl; Tsujimoto, Megumu

2017-02-01

Extreme environments sometimes support surprisingly high meiofaunal diversity. We sampled runoff from the acidic hot springs of Unzen, Japan. This is the type locality of Thermozodium esakii Rahm, 1937, the only tardigrade in the class Mesotardigrada, which remains contentious in the absence of corroboration or supporting specimens. Our sampling revealed at least three species of arthropods, four rotifers, and five nematodes living in the hot (ca. 40°C) and acidic (ca. pH 2.5) water, but no tardigrades.

Significance of Environmental Variables on Flight Electronics and Design Concerns for Extreme Environments

NASA Astrophysics Data System (ADS)

Hazeli, K.; Kingstedt, O. T.

2017-05-01

It is critical to investigate the performance of electronic systems and their components under the environments experienced during proposed missions to improve spacecraft and robotic vehicle functionality and performance in extreme environments.

Eukaryotic Organisms in Extreme Acidic Environments, the Río Tinto Case

NASA Astrophysics Data System (ADS)

Angeles Aguilera, Angeles

2013-07-01

A major issue in microbial ecology is to identify the limits of life for growth and survival, and to understand the molecular mechanisms that define these limits. Thus, interest in the biodiversity and ecology of extreme environments has grown in recent years for several reasons. Some are basic and revolve around the idea that extreme environments are believed to reflect early Earth conditions. Others are related to the biotechnological potential of extremophiles. In this regard, the study of extremely acidic environments has become increasingly important since environmental acidity is often caused by microbial activity. Highly acidic environments are relatively scarce worldwide and are generally associated with volcanic activity or mining operations. For most acidic environments, low pH facilitates metal solubility, and therefore acidic waters tend to have high concentrations of heavy metals. However, highly acidic environments are usually inhabited by acidophilic and acidotolerant eukaryotic microorganisms such as algae, amoebas, ciliates, heliozoan and rotifers, not to mention filamentous fungi and yeasts. Here, we review the general trends concerning the diversity and ecophysiology of eukaryotic acidophilic microorganims, as well as summarize our latest results on this topic in one of the largest extreme acidic rivers, Río Tinto (SW, Spain).

Extreme Programming in a Research Environment

NASA Technical Reports Server (NTRS)

Wood, William A.; Kleb, William L.

2002-01-01

This article explores the applicability of Extreme Programming in a scientific research context. The cultural environment at a government research center differs from the customer-centric business view. The chief theoretical difficulty lies in defining the customer to developer relationship. Specifically, can Extreme Programming be utilized when the developer and customer are the same person? Eight of Extreme Programming's 12 practices are perceived to be incompatible with the existing research culture. Further, six of the nine 'environments that I know don't do well with XP' apply. A pilot project explores the use of Extreme Programming in scientific research. The applicability issues are addressed and it is concluded that Extreme Programming can function successfully in situations for which it appears to be ill-suited. A strong discipline for mentally separating the customer and developer roles is found to be key for applying Extreme Programming in a field that lacks a clear distinction between the customer and the developer.

Insertion sequences enrichment in extreme Red sea brine pool vent.

PubMed

Elbehery, Ali H A; Aziz, Ramy K; Siam, Rania

2017-03-01

Mobile genetic elements are major agents of genome diversification and evolution. Limited studies addressed their characteristics, including abundance, and role in extreme habitats. One of the rare natural habitats exposed to multiple-extreme conditions, including high temperature, salinity and concentration of heavy metals, are the Red Sea brine pools. We assessed the abundance and distribution of different mobile genetic elements in four Red Sea brine pools including the world's largest known multiple-extreme deep-sea environment, the Red Sea Atlantis II Deep. We report a gradient in the abundance of mobile genetic elements, dramatically increasing in the harshest environment of the pool. Additionally, we identified a strong association between the abundance of insertion sequences and extreme conditions, being highest in the harshest and deepest layer of the Red Sea Atlantis II Deep. Our comparative analyses of mobile genetic elements in secluded, extreme and relatively non-extreme environments, suggest that insertion sequences predominantly contribute to polyextremophiles genome plasticity.

Strain corrosion cracking in rpm sewer piping

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hopkins, S.W.; Wachob, H.F.; Duffner, D.H.

1993-12-31

Long term, aggressive environmental exposure can result in localized failure of large diameter, glass reinforced plastic mortar (RPM) piping. In order to evaluate the performance of the liner and glass reinforced matrix polyester resin, accelerated strain corrosion tests were performed on samples of RPM piping that had already experienced almost 15 years of service. To assess the sensitivity of RPM pipe to acidic environments and to correlate the fractography of the laboratory produced failures with the excavated crack, short segments of 8-inch and 48-inch diameter piping were statically loaded to produce various known surface strains. After preloading the specimens tomore » fixed strain levels, these samples were then exposed to sulfuric acid solutions having pH values of 2.7 and 4.7 and monitored as a function of time until failure. The resulting lifetimes were related to initial surface strains and showed a decreasing logarithmic relationship. Fractographic examination of the excavated crack revealed the typical strain corrosion fractography of glass fibers after almost a 1000 hour exposure at 1.3 % strain; similar fractographic observations were obtained from failed laboratory samples. At shorter times, failure appeared to be overload in nature and exhibited little, if any, timedependent fracture features. Fractographic examination of the excavated crack strongly indicated that the crack had been present for a significant time. The extremely aggressive environment had totally dissolved the exposed glass reinforcement. Based on the laboratory strain corrosion performance, the nature of the contained cracking, and fractography of the failed surface, cracking of the excavated RPM pipe was believed to be the result of an early overload failure that subsequently propagated slowly via strain corrosion in an extremely aggressive environment.« less

Ultimately Reliable Pyrotechnic Systems

NASA Technical Reports Server (NTRS)

Scott, John H.; Hinkel, Todd

2015-01-01

This paper presents the methods by which NASA has designed, built, tested, and certified pyrotechnic devices for high reliability operation in extreme environments and illustrates the potential applications in the oil and gas industry. NASA's extremely successful application of pyrotechnics is built upon documented procedures and test methods that have been maintained and developed since the Apollo Program. Standards are managed and rigorously enforced for performance margins, redundancy, lot sampling, and personnel safety. The pyrotechnics utilized in spacecraft include such devices as small initiators and detonators with the power of a shotgun shell, detonating cord systems for explosive energy transfer across many feet, precision linear shaped charges for breaking structural membranes, and booster charges to actuate valves and pistons. NASA's pyrotechnics program is one of the more successful in the history of Human Spaceflight. No pyrotechnic device developed in accordance with NASA's Human Spaceflight standards has ever failed in flight use. NASA's pyrotechnic initiators work reliably in temperatures as low as -420 F. Each of the 135 Space Shuttle flights fired 102 of these initiators, some setting off multiple pyrotechnic devices, with never a failure. The recent landing on Mars of the Opportunity rover fired 174 of NASA's pyrotechnic initiators to complete the famous '7 minutes of terror.' Even after traveling through extreme radiation and thermal environments on the way to Mars, every one of them worked. These initiators have fired on the surface of Titan. NASA's design controls, procedures, and processes produce the most reliable pyrotechnics in the world. Application of pyrotechnics designed and procured in this manner could enable the energy industry's emergency equipment, such as shutoff valves and deep-sea blowout preventers, to be left in place for years in extreme environments and still be relied upon to function when needed, thus greatly enhancing safety and operational availability.

Isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments.

PubMed

La Duc, Myron T; Dekas, Anne; Osman, Shariff; Moissl, Christine; Newcombe, David; Venkateswaran, Kasthuri

2007-04-01

In assessing the bacterial populations present in spacecraft assembly, spacecraft test, and launch preparation facilities, extremophilic bacteria (requiring severe conditions for growth) and extremotolerant bacteria (tolerant to extreme conditions) were isolated. Several cultivation approaches were employed to select for and identify bacteria that not only survive the nutrient-limiting conditions of clean room environments but can also withstand even more inhospitable environmental stresses. Due to their proximity to spacefaring objects, these bacteria pose a considerable risk for forward contamination of extraterrestrial sites. Samples collected from four geographically distinct National Aeronautics and Space Administration clean rooms were challenged with UV-C irradiation, 5% hydrogen peroxide, heat shock, pH extremes (pH 3.0 and 11.0), temperature extremes (4 degrees C to 65 degrees C), and hypersalinity (25% NaCl) prior to and/or during cultivation as a means of selecting for extremotolerant bacteria. Culture-independent approaches were employed to measure viable microbial (ATP-based) and total bacterial (quantitative PCR-based) burdens. Intracellular ATP concentrations suggested a viable microbial presence ranging from below detection limits to 10(6) cells/m(2). However, only 0.1 to 55% of these viable cells were able to grow on defined culture medium. Isolated members of the Bacillaceae family were more physiologically diverse than those reported in previous studies, including thermophiles (Geobacillus), obligate anaerobes (Paenibacillus), and halotolerant, alkalophilic species (Oceanobacillus and Exiguobacterium). Non-spore-forming microbes (alpha- and beta-proteobacteria and actinobacteria) exhibiting tolerance to the selected stresses were also encountered. The multiassay cultivation approach employed herein enhances the current understanding of the physiological diversity of bacteria housed in these clean rooms and leads us to ponder the origin and means of translocation of thermophiles, anaerobes, and halotolerant alkalophiles into these environments.

Time-varying Concurrent Risk of Extreme Droughts and Heatwaves in California

NASA Astrophysics Data System (ADS)

Sarhadi, A.; Diffenbaugh, N. S.; Ausin, M. C.

2016-12-01

Anthropogenic global warming has changed the nature and the risk of extreme climate phenomena such as droughts and heatwaves. The concurrent of these nature-changing climatic extremes may result in intensifying undesirable consequences in terms of human health and destructive effects in water resources. The present study assesses the risk of concurrent extreme droughts and heatwaves under dynamic nonstationary conditions arising from climate change in California. For doing so, a generalized fully Bayesian time-varying multivariate risk framework is proposed evolving through time under dynamic human-induced environment. In this methodology, an extreme, Bayesian, dynamic copula (Gumbel) is developed to model the time-varying dependence structure between the two different climate extremes. The time-varying extreme marginals are previously modeled using a Generalized Extreme Value (GEV) distribution. Bayesian Markov Chain Monte Carlo (MCMC) inference is integrated to estimate parameters of the nonstationary marginals and copula using a Gibbs sampling method. Modelled marginals and copula are then used to develop a fully Bayesian, time-varying joint return period concept for the estimation of concurrent risk. Here we argue that climate change has increased the chance of concurrent droughts and heatwaves over decades in California. It is also demonstrated that a time-varying multivariate perspective should be incorporated to assess realistic concurrent risk of the extremes for water resources planning and management in a changing climate in this area. The proposed generalized methodology can be applied for other stochastic nature-changing compound climate extremes that are under the influence of climate change.

Microbial diversity in degraded and non-degraded petroleum samples and comparison across oil reservoirs at local and global scales.

PubMed

Sierra-Garcia, Isabel Natalia; Dellagnezze, Bruna M; Santos, Viviane P; Chaves B, Michel R; Capilla, Ramsés; Santos Neto, Eugenio V; Gray, Neil; Oliveira, Valeria M

2017-01-01

Microorganisms have shown their ability to colonize extreme environments including deep subsurface petroleum reservoirs. Physicochemical parameters may vary greatly among petroleum reservoirs worldwide and so do the microbial communities inhabiting these different environments. The present work aimed at the characterization of the microbiota in biodegraded and non-degraded petroleum samples from three Brazilian reservoirs and the comparison of microbial community diversity across oil reservoirs at local and global scales using 16S rRNA clone libraries. The analysis of 620 16S rRNA bacterial and archaeal sequences obtained from Brazilian oil samples revealed 42 bacterial OTUs and 21 archaeal OTUs. The bacterial community from the degraded oil was more diverse than the non-degraded samples. Non-degraded oil samples were overwhelmingly dominated by gammaproteobacterial sequences with a predominance of the genera Marinobacter and Marinobacterium. Comparisons of microbial diversity among oil reservoirs worldwide suggested an apparent correlation of prokaryotic communities with reservoir temperature and depth and no influence of geographic distance among reservoirs. The detailed analysis of the phylogenetic diversity across reservoirs allowed us to define a core microbiome encompassing three bacterial classes (Gammaproteobacteria, Clostridia, and Bacteroidia) and one archaeal class (Methanomicrobia) ubiquitous in petroleum reservoirs and presumably owning the abilities to sustain life in these environments.

Extreme Terrestrial Environments: Life in Thermal Stress and Hypoxia. A Narrative Review.

PubMed

Burtscher, Martin; Gatterer, Hannes; Burtscher, Johannes; Mairbäurl, Heimo

2018-01-01

Living, working and exercising in extreme terrestrial environments are challenging tasks even for healthy humans of the modern new age. The issue is not just survival in remote environments but rather the achievement of optimal performance in everyday life, occupation, and sports. Various adaptive biological processes can take place to cope with the specific stressors of extreme terrestrial environments like cold, heat, and hypoxia (high altitude). This review provides an overview of the physiological and morphological aspects of adaptive responses in these environmental stressors at the level of organs, tissues, and cells. Furthermore, adjustments existing in native people living in such extreme conditions on the earth as well as acute adaptive responses in newcomers are discussed. These insights into general adaptability of humans are complemented by outcomes of specific acclimatization/acclimation studies adding important information how to cope appropriately with extreme environmental temperatures and hypoxia.

Extreme Terrestrial Environments: Life in Thermal Stress and Hypoxia. A Narrative Review

PubMed Central

Burtscher, Martin; Gatterer, Hannes; Burtscher, Johannes; Mairbäurl, Heimo

2018-01-01

Living, working and exercising in extreme terrestrial environments are challenging tasks even for healthy humans of the modern new age. The issue is not just survival in remote environments but rather the achievement of optimal performance in everyday life, occupation, and sports. Various adaptive biological processes can take place to cope with the specific stressors of extreme terrestrial environments like cold, heat, and hypoxia (high altitude). This review provides an overview of the physiological and morphological aspects of adaptive responses in these environmental stressors at the level of organs, tissues, and cells. Furthermore, adjustments existing in native people living in such extreme conditions on the earth as well as acute adaptive responses in newcomers are discussed. These insights into general adaptability of humans are complemented by outcomes of specific acclimatization/acclimation studies adding important information how to cope appropriately with extreme environmental temperatures and hypoxia. PMID:29867589

Comparative genomic and plasmid analysis of beer-spoiling and non-beer-spoiling Lactobacillus brevis isolates.

PubMed

Bergsveinson, Jordyn; Ziola, Barry

2017-12-01

Beer-spoilage-related lactic acid bacteria (BSR LAB) belong to multiple genera and species; however, beer-spoilage capacity is isolate-specific and partially acquired via horizontal gene transfer within the brewing environment. Thus, the extent to which genus-, species-, or environment- (i.e., brewery-) level genetic variability influences beer-spoilage phenotype is unknown. Publicly available Lactobacillus brevis genomes were analyzed via BlAst Diagnostic Gene findEr (BADGE) for BSR genes and assessed for pangenomic relationships. Also analyzed were functional coding capacities of plasmids of LAB inhabiting extreme niche environments. Considerable genetic variation was observed in L. brevis isolated from clinical samples, whereas 16 candidate genes distinguish BSR and non-BSR L. brevis genomes. These genes are related to nutrient scavenging of gluconate or pentoses, mannose, and metabolism of pectin. BSR L. brevis isolates also have higher average nucleotide identity and stronger pangenome association with one another, though isolation source (i.e., specific brewery) also appears to influence the plasmid coding capacity of BSR LAB. Finally, it is shown that niche-specific adaptation and phenotype are plasmid-encoded for both BSR and non-BSR LAB. The ultimate combination of plasmid-encoded genes dictates the ability of L. brevis to survive in the most extreme beer environment, namely, gassed (i.e., pressurized) beer.

High Temperature Mechanisms for Venus Exploration

NASA Astrophysics Data System (ADS)

Ji, Jerri; Narine, Roop; Kumar, Nishant; Singh, Sase; Gorevan, Steven

Future Venus missions, including New Frontiers Venus In-Situ Explorer and three Flagship Missions - Venus Geophysical Network, Venus Mobile Explorer and Venus Surface Sample Return all focus on searching for evidence of past climate change both on the surface and in the atmospheric composition as well as in the interior dynamics of the planet. In order to achieve these goals and objectives, many key technologies need to be developed for the Venus extreme environment. These key technologies include sample acquisition systems and other high-temperature mechanisms and mobility systems capable of extended operation when directly exposed to the Venus surface or lower atmosphere environment. Honeybee Robotics has developed two types of high temperature motors, the materials and components in both motors were selected based on the requirement to survive temperatures above a minimum of 460° C, at earth atmosphere. The prototype Switched Reluctance Motor (SRM) has been operated non-continuously for over 20 hours at Venus-like conditions (460° C temperature, mostly CO2 gas environment) and it remains functional. A drilling system, actuated by two SRMs was tested in Venus-like conditions, 460° C temperature and mostly CO2 gas environment, for more than 15 hours. The drill successfully completed three tests by drilling into chalk up to 6 inches deep in each test. A first generation Brushless DC (BLDC) Motor and high temperature resolver were also tested and the feasibility of the designs was demonstrated by the extended operation of both devices under Venus-like condition. Further development of the BLDC motor and resolver continues and these devices will, ultimately, be integrated into the development of a high temperature sample acquisition scoop and high temperature joint (awarded SBIR Phase II in October, 2007). Both the SR and BLDC motors will undergo extensive testing at Venus temperature and pressure (TRL6) and are expected to be mission ready before the next New Frontiers AO release. Scalable high temperature motor, resolver and bearing developments allow for creation of long lasting sample acquisition systems, booms, robot arms and even mobility systems that operate outside of an environment-controlled landed platform on the surface of Venus. The SR and BLDC motors are no longer expected to limit the life of Venus surface operations. With the accompanying high temperature bearing and other mechanisms development, surface operations will be limited only by available power. Therefore, the motor and resolver's capability to survive for hours (and potentially longer) in the environment is a major benefit to future Venus science missions and they also allow time for communication ground loops to optimize sample target selection and the possibility for acquiring multiple samples from the surface. The extreme temperature motors, resolver and other high temperature mechanisms therefore revolutionize the exploration of Venus.

The Competing Influences of the Radiation Belts on the Charging of Extremely Resistive Spacecraft Materials

NASA Astrophysics Data System (ADS)

Lemon, C.; Roeder, J. L.; Looper, M. D.; O'Brien, T. P., III; Fennell, J. F.; Mazur, J. E.

2016-12-01

Spacecraft suffer from various types of anomalies caused by space weather. One important source of spacecraft anomalies is internal electrostatic discharge (IESD), which occurs when penetrating electrons deposit charge inside dielectrics faster than that charge can dissipate via conduction currents. This causes the electric field to build up to a breakdown threshold. The most electrically resistive materials, such as Teflon, are of greatest concern for IESD. Laboratory measurements of the conductivity of Teflon and other highly resistive polymers show that their conventional conductivity is negligible in comparison to their radiation-induced conductivity (RIC), an alternate source of conduction that is linearly proportional to the ionizing dose rate received by the material. The space radiation environment therefore plays contradictory roles in extremely resistive polymers, both depositing charge and dissipating it. The spectral shape, rather than the total electron flux, becomes the primary consideration for IESD because it determines the relative deposition of charge and ionizing dose in materials. A counterintuitive result is that soft spectra may be a greater risk for IESD, because relative to hard spectra they deposit more charge than dose in materials. This differs from the standard practice of defining the worst-possible environment for charging and IESD as the spectrum in which the electron flux is highest at all energies that could reach the material. We present analyses of CRRES MEA and HEEF measurements, and simulate the charging of material samples from the CRRES Internal Discharge Monitor. We briefly demonstrate the unexpected results described here, and quantify the effect of different energetic electron spectra observed by CRRES on the buildup of charge in Teflon samples from the Internal Discharge Monitor. Finally, we will comment on the perceived deficiency of "worst case" charging environments for predicting IESD, and how we can better quantify IESD risk in extremely resistive materials.

Time-of-flight Extreme Environment Diffractometer at the Helmholtz-Zentrum Berlin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prokhnenko, Oleksandr, E-mail: prokhnenko@helmholtz-berlin.de; Stein, Wolf-Dieter; Bleif, Hans-Jürgen

2015-03-15

The Extreme Environment Diffractometer (EXED) is a new neutron time-of-flight instrument at the BER II research reactor at the Helmholtz-Zentrum Berlin, Germany. Although EXED is a special-purpose instrument, its early construction made it available for users as a general-purpose diffractometer. In this respect, EXED became one of the rare examples, where the performance of a time-of-flight diffractometer at a continuous source can be characterized. In this paper, we report on the design and performance of EXED with an emphasis on the unique instrument capabilities. The latter comprise variable wavelength resolution and wavelength band, control of the incoming beam divergence, themore » possibility to change the angular positions of detectors and their distance to the sample, and use of event recording and offline histogramming. These features combined make EXED easily tunable to the requirements of a particular problem, from conventional diffraction to small angle neutron scattering. The instrument performance is demonstrated by several reference measurements and user experiments.« less

Silicified virus-like nanoparticles in an extreme thermal environment: implications for the preservation of viruses in the geological record.

PubMed

Peng, X; Xu, H; Jones, B; Chen, S; Zhou, H

2013-11-01

Biofilms that grow around Gumingquan hot spring (T = 71 °C, pH = 9.2) in the Rehai geothermal area, Tengchong, China, are formed of various cyanobacteria, Firmicutes, Aquificae, Thermodesulfobacteria, Desulfurococcales, and Thermoproteales. Silicified virus-like nanoparticles, 40-200 nm in diameter, are common inside the microbial cells and the extracellular polymeric substances around the cells. These nanoparticles, which are formed of a core encased by a silica cortex, are morphologically akin to known viruses and directly comparable to silicified virus-like particles that were produced in biofilms cultured in the laboratory. The information obtained from examination of the natural and laboratory-produced samples suggests that viruses can be preserved by silicification, especially while they are still encased in their host cells. These results expand our views of virus-host mineral interaction in extreme thermal environments and imply that viruses can be potentially preserved and identified in the geological record. © 2013 John Wiley & Sons Ltd.

Ubiquitous giants: a plethora of giant viruses found in Brazil and Antarctica.

PubMed

Andrade, Ana Cláudia Dos S P; Arantes, Thalita S; Rodrigues, Rodrigo A L; Machado, Talita B; Dornas, Fábio P; Landell, Melissa F; Furst, Cinthia; Borges, Luiz G A; Dutra, Lara A L; Almeida, Gabriel; Trindade, Giliane de S; Bergier, Ivan; Abrahão, Walter; Borges, Iara A; Cortines, Juliana R; de Oliveira, Danilo B; Kroon, Erna G; Abrahão, Jônatas S

2018-01-24

Since the discovery of giant viruses infecting amoebae in 2003, many dogmas of virology have been revised and the search for these viruses has been intensified. Over the last few years, several new groups of these viruses have been discovered in various types of samples and environments.In this work, we describe the isolation of 68 giant viruses of amoeba obtained from environmental samples from Brazil and Antarctica. Isolated viruses were identified by hemacolor staining, PCR assays and electron microscopy (scanning and/or transmission). A total of 64 viruses belonging to the Mimiviridae family were isolated (26 from lineage A, 13 from lineage B, 2 from lineage C and 23 from unidentified lineages) from different types of samples, including marine water from Antarctica, thus being the first mimiviruses isolated in this extreme environment to date. Furthermore, a marseillevirus was isolated from sewage samples along with two pandoraviruses and a cedratvirus (the third to be isolated in the world so far). Considering the different type of samples, we found a higher number of viral groups in sewage samples. Our results reinforce the importance of prospective studies in different environmental samples, therefore improving our comprehension about the circulation anddiversity of these viruses in nature.

European Research on life in extreme environments, setting up research priorities and coordinating the community: the CAREX project

NASA Astrophysics Data System (ADS)

Rettberg, P.; Ellis-Evans, C.; Prieur, D.; Loreto, F.; Walter, N.; Le Bris, N.; Elster, J.; Amils, R.; Marteinsson, V.

2008-09-01

Life in Extreme Environments is an emerging area of research in which Europe has considerable expertise but a relatively fragmented research infrastructure. The science of such environments has enormous relevance for our knowledge of the diversity and environmental limits of microbial, plant and animal life and the novel strategies employed for survival and growth. Such studies are essential in understanding how life established on the early Earth and in assessing the possibilities for life on other planetary bodies. These environments are also a rich source of novel exploitable compounds. At the European level, there is a need for better coordination of life in extreme environments research, the FP7-funded CAREX project aims to address this need by developing a clearly identifiable, dynamic and durable community. Establishing this community will encourage greater interdisciplinarity and increasing knowledge of extreme environments. It will provide a target for young career scientists and allow a more focussed dialogue with other science areas, with funding agencies, with industrial groups and with international organisations outside Europe. CAREX will last for three years and with a wide scope covering microbial life, plant adaptation and animal adaptation to various marine, polar, terrestrial extreme environments as well as outer space. CAREX's outputs will include a strategic roadmap for European life in extreme environments research (including enabling technologies), diverse opportunities for knowledge transfer, standardisation of methodologies, encouragement and support for early career scientists and a network of links to relevant organisations. These deliverables together with improved community networking, supported by newsletters, promotional leaflets, a series of science publications and an interactive web portal, will help consolidate the community and its identity. Outcomes will be facilitated through science/technology workshops, diverse forums, field/laboratory protocol intercomparisons, a summer school and individual grants to facilitate knowledge transfer. CAREX has evolved with the key players from the highly successful ESF "Investigating Life in Extreme Environments" initiative. For more information: www.carex-eu.org

Neutron Time-of-Flight Diffractometer HIPPO at LANSCE

NASA Astrophysics Data System (ADS)

Vogel, Sven; Williams, Darrick; Zhao, Yusheng; Bennett, Kristin; von Dreele, Bob; Wenk, Hans-Rudolf

2004-03-01

The High-Pressure Preferred Orientation (HIPPO) neutron diffractometer is the first third-generation neutron time-of-flight powder diffractometer to be constructed in the United States. It produces extremely high intensity by virtue of a short (9 m) initial flight path on a high intensity water moderator and 1380 3He detector tubes covering 4.5 m2 of detector area from 10' to 150' in scattering angles. HIPPO was designed and manufactured as a joint effort between LANSCE and University of California with the goals of attaining world-class science and making neutron powder diffractometry an accessible and available tool to the national user community. Over two decades of momentum transfer are available (0.1-30 A-1) to support studies of amorphous solids; magnetic diffraction; small crystalline samples; and samples subjected to extreme environments such as temperature, pressure, or magnetic fields. The exceptionally high data rates of HIPPO also make it useful for time-resolved studies. In addition to the standard ancillary equipment (100-position sample/texture changer, closed-cycle He refrigerator, furnace), HIPPO has unique high-pressure cells capable of achieving pressures of 30 GPA at ambient and high (2000 K) temperature with samples up to 100 mm3 in volume.

Patterns of Arbuscular Mycorrhizal Fungal Distribution on Mainland and Island Sandy Coastal Plain Ecosystems in Brazil.

PubMed

da Silva, Iolanda Ramalho; de Souza, Francisco Adriano; da Silva, Danielle Karla Alves; Oehl, Fritz; Maia, Leonor Costa

2017-10-01

Although sandy coastal plains are important buffer zones to protect the coast line and maintain biological diversity and ecosystem services, these ecosystems have been endangered by anthropogenic activities. Thus, information on coastal biodiversity and forces shaping coastal biological diversity are extremely important for effective conservation strategies. In this study, we aimed to compare arbuscular mycorrhizal (AM) fungal communities from soil samples collected on the mainland and nearby islands located in Brazilian sandy coastal plain ecosystems (Restingas) to get information about AM fungal biogeography and identify factors shaping these communities. Soil samples were collected in 2013 and 2014 on the beachfront of the tropical sandy coastal plain at six sites (three island and three mainland locations) across the northeast, southeast, and south regions of Brazil. Overall, we recorded 53 AM fungal species from field and trap culture samples. The richness and diversity of AM fungal species did not differ between mainland and island locations, but AM fungal community assemblages were different between mainland and island environments and among most sites sampled. Glomeromycota communities registered from island samples showed higher heterogeneity than communities from mainland samples. Sandy coastal plains harbor diverse AM fungal communities structured by climatic, edaphic, and spatial factors, while the distance from the colonizing source (mainland environments) does not strongly affect the AM fungal communities in Brazilian coastal environments.

Amchitka Island, Alaska, special sampling project 1997

DOE Office of Scientific and Technical Information (OSTI.GOV)

U.S. Department of Energy, Nevada Operations Office

2000-06-28

This 1997 special sampling project represents a special radiobiological sampling effort to augment the 1996 Long-Term Hydrological Monitoring Program (LTHMP) for Amchitka Island in Alaska. Lying in the western portion of the Aleutian Islands arc, near the International Date Line, Amchitka Island is one of the southernmost islands of the Rat Island Chain. Between 1965 and 1971, the U.S. Atomic Energy Commission conducted three underground nuclear tests on Amchitka Island. In 1996, Greenpeace collected biota samples and speculated that several long-lived, man-made radionuclides detected (i.e., americium-241, plutonium-239 and -240, beryllium-7, and cesium-137) leaked into the surface environment from underground cavitiesmore » created during the testing. The nuclides of interest are detected at extremely low concentrations throughout the environment. The objectives of this special sampling project were to scientifically refute the Greenpeace conclusions that the underground cavities were leaking contaminants to the surface. This was achieved by first confirming the presence of these radionuclides in the Amchitka Island surface environment and, second, if the radionuclides were present, determining if the source is the underground cavity or worldwide fallout. This special sampling and analysis determined that the only nonfallout-related radionuclide detected was a low level of tritium from the Long Shot test, which had been previously documented. The tritium contamination is monitored and continues a decreasing trend due to radioactive decay and dilution.« less

40 CFR 355.12 - What quantities of extremely hazardous substances trigger emergency planning requirements?

Code of Federal Regulations, 2010 CFR

2010-07-01

... EMERGENCY PLANNING AND NOTIFICATION Emergency Planning Who Must Comply § 355.12 What quantities of extremely... 40 Protection of Environment 27 2010-07-01 2010-07-01 false What quantities of extremely hazardous substances trigger emergency planning requirements? 355.12 Section 355.12 Protection of Environment...

Body proportions of circumpolar peoples as evidenced from skeletal data: Ipiutak and Tigara (Point Hope) versus Kodiak Island Inuit.

PubMed

Holliday, Trenton W; Hilton, Charles E

2010-06-01

Given the well-documented fact that human body proportions covary with climate (presumably due to the action of selection), one would expect that the Ipiutak and Tigara Inuit samples from Point Hope, Alaska, would be characterized by an extremely cold-adapted body shape. Comparison of the Point Hope Inuit samples to a large (n > 900) sample of European and European-derived, African and African-derived, and Native American skeletons (including Koniag Inuit from Kodiak Island, Alaska) confirms that the Point Hope Inuit evince a cold-adapted body form, but analyses also reveal some unexpected results. For example, one might suspect that the Point Hope samples would show a more cold-adapted body form than the Koniag, given their more extreme environment, but this is not the case. Additionally, univariate analyses seldom show the Inuit samples to be more cold-adapted in body shape than Europeans, and multivariate cluster analyses that include a myriad of body shape variables such as femoral head diameter, bi-iliac breadth, and limb segment lengths fail to effectively separate the Inuit samples from Europeans. In fact, in terms of body shape, the European and the Inuit samples tend to be cold-adapted and tend to be separated in multivariate space from the more tropically adapted Africans, especially those groups from south of the Sahara. Copyright 2009 Wiley-Liss, Inc.

Looking for Life in Extreme Environments on Earth and Beyond: Professional Development Workshop for Educators

NASA Astrophysics Data System (ADS)

Droppo, R.; Pratt, L.; Suchecki, P. C.

2010-08-01

The Looking for Life in Extreme Environments workshop held at Indiana University Bloomington in July of 2009 was the first in a series of workshops for high-school teachers that are currently in development. The workshops' modules are based on the research of faculty members in the Departments of Geological Sciences, Biology, and Astronomy, the School of Education, and the School of Public and Environmental Affairs at Indiana University Bloomington; the modules use lessons from Exploring Deep-Subsurface Life. Earth Analogues for Possible Life on Mars: Lessons and Activities, curricular materials that were produced and edited by Lisa Pratt and Ruth Droppo and published by NASA in 2008. Exploring Deep-Subsurface Life is a workbook, a DVD (with closed-captioning), and a CD with the lessons in digital text format for adaptation to classroom needs and printing. Each lesson includes the National Education Standards that apply to the materials. The workbook's lessons are written with three considerations: Life Domains, Cellular Metabolism, and Extreme Environments and Microbes. Students are challenged to build, draw, measure, discuss, and participate in laboratory processes and experiments that help them understand and describe microbes and their environments. In the Capstone, the students write a grant proposal based on the three lessons' analogues. The DVD is collection of videotaped interviews with scientists in laboratories at Michigan State, Princeton, and Indiana University, who are working on water and gas samples they collected from deep gold mines in South Africa and the Canadian Arctic. The interview materials and some animated graphics are compiled into four video pieces that support and compliment the accompanying workbook lessons and activities, and offer students insight into the excitement of scientific discovery.

Sediment Dynamics Within Buffer Zone and Sinkhole Splay Areas Under Extreme Soil Disturbance Conditions.

PubMed

Schoonover, Jon E; Crim, Jackie F; Williard, Karl W J; Groninger, John W; Zaczek, James J; Pattumma, Klairoong

2015-09-01

Sedimentation dynamics were assessed in sinkholes within training areas at Ft. Knox Military Installation, a karst landscape subjected to decades of tracked vehicle use and extreme soil disturbance. Sinkholes sampled were sediment-laden and behaved as intermittent ponds. Dendrogeomorphic analyses were conducted using willow trees (Salix spp.) located around the edge of 18 sinkholes to estimate historical sedimentation rates, and buried bottles were installed in 20 sinkholes at the center, outer edge, and at the midpoint between the center and edge to estimate annual sedimentation rates. Sedimentation data were coupled with vegetation characteristics of sinkhole buffers to determine relationships among these variables. The dendrogeomorphic method estimated an average accumulation rate of 1.27 cm year(-1) translating to a sediment loss rate of 46.1 metric ton year(-1) from the training areas. However, sediment export to sinkholes was estimated to be much greater (118.6 metric ton year(-1)) via the bottle method. These data suggest that the latter method provided a more accurate estimate since accumulation was greater in the center of sinkholes compared to the periphery where dendrogeomorphic data were collected. Vegetation data were not tightly correlated with sedimentation rates, suggesting that further research is needed to identify a viable proxy for direct measures of sediment accumulation in this extreme deposition environment. Mitigation activities for the sinkholes at Ft. Knox's tank training area, and other heavily disturbed karst environments where extreme sedimentation exists, should consider focusing on flow path and splay area management.

A Motor Drive Electronics Assembly for Mars Curiosity Rover: An Example of Assembly Qualification for Extreme Environments

NASA Technical Reports Server (NTRS)

Kolawa, Elizabeth; Chen, Yuan; Mojarradi, Mohammad M.; Weber, Carissa Tudryn; Hunter, Don J.

2013-01-01

This paper describes the technology development and infusion of a motor drive electronics assembly for Mars Curiosity Rover under space extreme environments. The technology evaluation and qualification as well as space qualification of the assembly are detailed and summarized. Because of the uncertainty of the technologies operating under the extreme space environments and that a high level reliability was required for this assembly application, both component and assembly board level qualifications were performed.

[Determination of total mercury in water samples, sediments and solids in suspension in aquatic systems by cold-vapor atomic absorption spectrophotometry].

PubMed

Vieira, J L; Passarelli, M M

1996-06-01

The use of metallic mercury in the extraction and concentration of gold causes the discarding of tons of this metal in the environment, leading to a considerable increase in the natural levels of the same and the contamination of the surrounding areas. Thus it is extremely important to monitor the presence of this metal in various sectors of the environment with a view aiming to preventing human exposure to excessive concentrations which can result in serious episodes of mercury poisoning. It is also important to estimate the possibility of river sediments becoming potential sources of contamination of human beings. The determination of total mercury was undertaken by using cold vapor atomic absorption spectrometry. River waters, as well as sediments and suspended solids were used as samples for the standardization of the analytical procedure. Later on, this method was tested on samples originating in gold mining areas for the purpose of assessing its validity.

Sample Handling in Extreme Environments

NASA Technical Reports Server (NTRS)

Avellar, Louisa; Badescu, Mircea; Sherrit, Stewart; Bar-Cohen, Yoseph

2013-01-01

Harsh environments, such as that on Venus, preclude the use of existing equipment for functions that involve interaction with the environment. The operating limitations of current high temperature electronics are well below the actual temperature and pressure found on Venus (460 deg C and 92 atm), so proposed lander configurations typically include a pressure vessel where the science instruments are kept at Earth-like temperature and pressure (25 deg C and 1 atm). The purpose of this project was to develop and demonstrate a method for sample transfer from an external drill to internal science instruments for a lander on Venus. The initial concepts were string and pneumatically driven systems; and the latter system was selected for its ability to deliver samples at very high speed. The pneumatic system was conceived to be driven by the pressure difference between the Venusian atmosphere and the inside of the lander. The pneumatic transfer of a small capsule was demonstrated, and velocity data was collected from the lab experiment. The sample transfer system was modeled using CAD software and prototyped using 3D printing. General structural and thermal analyses were performed to approximate the proposed system's mass and effects on the temperature and pressure inside of the lander. Additionally, a sampler breadboard for use on Titan was tested and functionality problems were resolved.

Fiber Bragg Grating Dilatometry in Extreme Magnetic Field and Cryogenic Conditions

PubMed Central

Corvalán Moya, Carolina; Weickert, Franziska; Zapf, Vivien; Balakirev, Fedor F.; Wartenbe, Mark; Rosa, Priscila F. S.; Betts, Jonathan B.; Crooker, Scott A.; Daou, Ramzy

2017-01-01

In this work, we review single mode SiO2 fiber Bragg grating techniques for dilatometry studies of small single-crystalline samples in the extreme environments of very high, continuous, and pulsed magnetic fields of up to 150 T and at cryogenic temperatures down to <1 K. Distinct millimeter-long materials are measured as part of the technique development, including metallic, insulating, and radioactive compounds. Experimental strategies are discussed for the observation and analysis of the related thermal expansion and magnetostriction of materials, which can achieve a strain sensitivity (ΔL/L) as low as a few parts in one hundred million (≈10−8). The impact of experimental artifacts, such as those originating in the temperature dependence of the fiber’s index of diffraction, light polarization rotation in magnetic fields, and reduced strain transfer from millimeter-long specimens, is analyzed quantitatively using analytic models available in the literature. We compare the experimental results with model predictions in the small-sample limit, and discuss the uncovered discrepancies. PMID:29117137

Fiber Bragg Grating Dilatometry in Extreme Magnetic Field and Cryogenic Conditions.

PubMed

Jaime, Marcelo; Corvalán Moya, Carolina; Weickert, Franziska; Zapf, Vivien; Balakirev, Fedor F; Wartenbe, Mark; Rosa, Priscila F S; Betts, Jonathan B; Rodriguez, George; Crooker, Scott A; Daou, Ramzy

2017-11-08

In this work, we review single mode SiO₂ fiber Bragg grating techniques for dilatometry studies of small single-crystalline samples in the extreme environments of very high, continuous, and pulsed magnetic fields of up to 150 T and at cryogenic temperatures down to <1 K. Distinct millimeter-long materials are measured as part of the technique development, including metallic, insulating, and radioactive compounds. Experimental strategies are discussed for the observation and analysis of the related thermal expansion and magnetostriction of materials, which can achieve a strain sensitivity ( ΔL/L ) as low as a few parts in one hundred million (≈10 -8 ). The impact of experimental artifacts, such as those originating in the temperature dependence of the fiber's index of diffraction, light polarization rotation in magnetic fields, and reduced strain transfer from millimeter-long specimens, is analyzed quantitatively using analytic models available in the literature. We compare the experimental results with model predictions in the small-sample limit, and discuss the uncovered discrepancies.

Fiber Bragg Grating Dilatometry in Extreme Magnetic Field and Cryogenic Conditions

DOE PAGES

Jaime, Marcelo; Corvalán Moya, Carolina; Weickert, Franziska; ...

2017-11-08

In this work, we review single mode SiO 2 fiber Bragg grating techniques for dilatometry studies of small single-crystalline samples in the extreme environments of very high, continuous, and pulsed magnetic fields of up to 150 T and at cryogenic temperatures down to <1 K. Distinct millimeter-long materials are measured as part of the technique development, including metallic, insulating, and radioactive compounds. Experimental strategies are discussed for the observation and analysis of the related thermal expansion and magnetostriction of materials, which can achieve a strain sensitivity (ΔL/L) as low as a few parts in one hundred million (≈10 -8). Themore » impact of experimental artifacts, such as those originating in the temperature dependence of the fiber’s index of diffraction, light polarization rotation in magnetic fields, and reduced strain transfer from millimeter-long specimens, is analyzed quantitatively using analytic models available in the literature. We compare the experimental results with model predictions in the small-sample limit, and discuss the uncovered discrepancies.« less

Development of a Temperature Sensor for Jet Engine and Space Missions Environments

NASA Technical Reports Server (NTRS)

Patterson, Richard L.; Hammoud, Ahmad; Culley, Dennis E.; Elbuluk, Malik

2008-01-01

Electronic systems in aerospace and in space exploration missions are expected to encounter extreme temperatures and wide thermal swings. To address the needs for extreme temperature electronics, research efforts exist at the NASA Glenn Research Center (GRC) to develop and evaluate electronics for extreme temperature operations, and to establish their reliability under extreme temperature operation and thermal cycling; conditions that are typical of both the aerospace and space environments. These efforts are supported by the NASA Fundamental Aeronautics/Subsonic Fixed Wing Program and by the NASA Electronic Parts and Packaging (NEPP) Program. This work reports on the results obtained on the development of a temperature sensor geared for use in harsh environments.

Examining personal values in extreme environment contexts: Revisiting the question of generalizability

NASA Astrophysics Data System (ADS)

Smith, N.; Sandal, G. M.; Leon, G. R.; Kjærgaard, A.

2017-08-01

Land-based extreme environments (e.g. polar expeditions, Antarctic research stations, confinement chambers) have often been used as analog settings for spaceflight. These settings share similarities with the conditions experienced during space missions, including confinement, isolation and limited possibilities for evacuation. To determine the utility of analog settings for understanding human spaceflight, researchers have examined the extent to which the individual characteristics (e.g., personality) of people operating in extreme environments can be generalized across contexts (Sandal, 2000) [1]. Building on previous work, and utilising new and pre-existing data, the present study examined the extent to which personal value motives could be generalized across extreme environments. Four populations were assessed; mountaineers (N =59), military personnel (N = 25), Antarctic over-winterers (N = 21) and Mars simulation participants (N = 12). All participants completed the Portrait Values Questionnaire (PVQ; Schwartz; 2) capturing information on 10 personal values. Rank scores suggest that all groups identified Self-direction, Stimulation, Universalism and Benevolence as important values and acknowledged Power and Tradition as being low priorities. Results from difference testing suggest the extreme environment groups were most comparable on Self-direction, Stimulation, Benevolence, Tradition and Security. There were significant between-group differences on five of the ten values. Overall, findings pinpointed specific values that may be important for functioning in challenging environments. However, the differences that emerged on certain values highlight the importance of considering the specific population when comparing results across extreme settings. We recommend that further research examine the impact of personal value motives on indicators of adjustment, group working, and performance. Information from such studies could then be used to aid selection and training processes for personnel operating in extreme settings, and in space.

Searching for life in extreme environments relevant to Jovian's Europa: Lessons from subglacial ice studies at Lake Vostok (East Antarctica)

NASA Astrophysics Data System (ADS)

Bulat, Sergey A.; Alekhina, Irina A.; Marie, Dominique; Martins, Jean; Petit, Jean Robert

2011-08-01

The objective was to estimate the genuine microbial content of ice samples from refrozen water (accretion ice) from the subglacial Lake Vostok (Antarctica) buried beneath the 4-km thick East Antarctic ice sheet. The samples were extracted by heavy deep ice drilling from 3659 m below the surface. High pressure, a low carbon and chemical content, isolation, complete darkness and the probable excess of oxygen in water for millions of years characterize this extreme environment. A decontamination protocol was first applied to samples selected for the absence of cracks to remove the outer part contaminated by handling and drilling fluid. Preliminary indications showed the accretion ice samples to be almost gas free with a low impurity content. Flow cytometry showed the very low unevenly distributed biomass while repeated microscopic observations were unsuccessful.We used strategies of Ancient DNA research that include establishing contaminant databases and criteria to validate the amplification results. To date, positive results that passed the artifacts and contaminant databases have been obtained for a pair of bacterial phylotypes only in accretion ice samples featured by some bedrock sediments. The phylotypes included the chemolithoautotrophic thermophile Hydrogenophilus thermoluteolus and one unclassified phylotype. Combined with geochemical and geophysical considerations, our results suggest the presence of a deep biosphere, possibly thriving within some active faults of the bedrock encircling the subglacial lake, where the temperature is as high as 50 °C and in situ hydrogen is probably present.Our approach indicates that the search for life in the subglacial Lake Vostok is constrained by a high probability of forward-contamination. Our strategy includes strict decontamination procedures, thorough tracking of contaminants at each step of the analysis and validation of the results along with geophysical and ecological considerations for the lake setting. This may serve to establish a guideline protocol for studying extraterrestrial ice samples.

Embedded I&C for Extreme Environments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kisner, Roger A.

2016-04-01

This project uses embedded instrumentation and control (I&C) technologies to demonstrate potential performance gains of nuclear power plant components in extreme environments. Extreme environments include high temperature, radiation, high pressure, high vibration, and high EMI conditions. For extreme environments, performance gains arise from moment-to-moment sensing of local variables and immediate application of local feedback control. Planning for embedding I&C during early system design phases contrasts with the traditional, serial design approach that incorporates minimal I&C after mechanical and electrical design is complete. The demonstration application involves the development and control of a novel, proof-of-concept motor/pump design. The motor and pumpmore » combination operate within the fluid environment, eliminating the need for rotating seals. Actively controlled magnetic bearings also replace failure-prone mechanical contact bearings that typically suspend rotating components. Such as design has the potential to significantly enhance the reliability and life of the pumping system and would not be possible without embedded I&C.« less

On the edge of a deep biosphere: Real animals in extreme environments

NASA Astrophysics Data System (ADS)

Childress, James J.; Fisher, Charles F.; Felbeck, Horst; Girguis, Peter

This paper considers the possibility of animals living in a subsurface environment on the global mid-ocean ridge system. It considers the possible environments and looks at the possibilities of animal inhabitants of the subsurface biosphere based on adaptations of animals to other extreme habitats. We conclude that there are known bridging inhabitants of the subsurface biosphere, that part-time inhabitants are extremely likely, and that there could be full-time inhabitants if conditions are stable within the tolerance limits of metazoans for time periods of months.

Soil Microbial Responses to Increased Moisture and Organic Resources along a Salinity Gradient in a Polar Desert

PubMed Central

Van Horn, David J.; Okie, Jordan G.; Buelow, Heather N.; Gooseff, Michael N.; Barrett, John E.

2014-01-01

Microbial communities in extreme environments often have low diversity and specialized physiologies suggesting a limited resistance to change. The McMurdo Dry Valleys (MDV) are a microbially dominated, extreme ecosystem currently undergoing climate change-induced disturbances, including the melting of massive buried ice, cutting through of permafrost by streams, and warming events. These processes are increasing moisture across the landscape, altering conditions for soil communities by mobilizing nutrients and salts and stimulating autotrophic carbon inputs to soils. The goal of this study was to determine the effects of resource addition (water/organic matter) on the composition and function of microbial communities in the MDV along a natural salinity gradient representing an additional gradient of stress in an already extreme environment. Soil respiration and the activity of carbon-acquiring extracellular enzymes increased significantly (P < 0.05) with the addition of resources at the low- and moderate-salinity sites but not the high-salinity site. The bacterial community composition was altered, with an increase in Proteobacteria and Firmicutes with water and organic matter additions at the low- and moderate-salinity sites and a near dominance of Firmicutes at the high-salinity site. Principal coordinate analyses of all samples using a phylogenetically informed distance matrix (UniFrac) demonstrated discrete clustering among sites (analysis of similarity [ANOSIM], P < 0.05 and R > 0.40) and among most treatments within sites. The results from this experimental work suggest that microbial communities in this environment will undergo rapid change in response to the altered resources resulting from climate change impacts occurring in this region. PMID:24610850

Soil microbial responses to increased moisture and organic resources along a salinity gradient in a polar desert.

PubMed

Van Horn, David J; Okie, Jordan G; Buelow, Heather N; Gooseff, Michael N; Barrett, John E; Takacs-Vesbach, Cristina D

2014-05-01

Microbial communities in extreme environments often have low diversity and specialized physiologies suggesting a limited resistance to change. The McMurdo Dry Valleys (MDV) are a microbially dominated, extreme ecosystem currently undergoing climate change-induced disturbances, including the melting of massive buried ice, cutting through of permafrost by streams, and warming events. These processes are increasing moisture across the landscape, altering conditions for soil communities by mobilizing nutrients and salts and stimulating autotrophic carbon inputs to soils. The goal of this study was to determine the effects of resource addition (water/organic matter) on the composition and function of microbial communities in the MDV along a natural salinity gradient representing an additional gradient of stress in an already extreme environment. Soil respiration and the activity of carbon-acquiring extracellular enzymes increased significantly (P < 0.05) with the addition of resources at the low- and moderate-salinity sites but not the high-salinity site. The bacterial community composition was altered, with an increase in Proteobacteria and Firmicutes with water and organic matter additions at the low- and moderate-salinity sites and a near dominance of Firmicutes at the high-salinity site. Principal coordinate analyses of all samples using a phylogenetically informed distance matrix (UniFrac) demonstrated discrete clustering among sites (analysis of similarity [ANOSIM], P < 0.05 and R > 0.40) and among most treatments within sites. The results from this experimental work suggest that microbial communities in this environment will undergo rapid change in response to the altered resources resulting from climate change impacts occurring in this region.

From Local to EXtreme Environments (FLEXE): Promoting Earth Systems Science Literacy Through Student Inquiry and Real Data

NASA Astrophysics Data System (ADS)

Goehring, E. C.; Carlsen, W.; Larsen, J.; Simms, E.; Smith, M.

2007-12-01

From Local to EXtreme Environments (FLEXE) is an innovative new project of the GLOBE Program that involves middle and high school students in systematic, facilitated analyses and comparisons of real environmental data. Through FLEXE, students collect and analyze data from various sources, including the multi-year GLOBE database, deep-sea scientific research projects, and direct measurements of the local environment collected by students using GLOBE sampling protocols. Initial FLEXE materials and training have focused on student understanding of energy transfer through components of the Earth system, including a comparison of how local environmental conditions differ from those found at deep-sea hydrothermal vent communities. While the importance of data acquisition, accuracy and replication is emphasized, FLEXE is also uniquely structured to deepen students' understanding of multiple aspects of the process and nature of science, including written communication of results and on-line peer review. Analyses of data are facilitated through structured, web-based interactions and culminating activities with at-sea scientists through an online forum. The project benefits from the involvement of a professional evaluator, and as the model is tested and refined, it may serve as a template for the inclusion of additional "extreme" earth systems. FLEXE is a partnership of the international GLOBE web- based education program and the NSF Ridge 2000 mid-ocean ridge and hydrothermal vent research program, and includes the expertise of the Center for Science and the Schools at Penn State University. International collaborators also include the InterRidge and ChEss international research programs.

Magneto-optical imaging technique for hostile environments: The ghost imaging approach

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meda, A.; Caprile, A.; Avella, A.

2015-06-29

In this paper, we develop an approach to magneto optical imaging (MOI), applying a ghost imaging (GI) protocol to perform Faraday microscopy. MOI is of the utmost importance for the investigation of magnetic properties of material samples, through Weiss domains shape, dimension and dynamics analysis. Nevertheless, in some extreme conditions such as cryogenic temperatures or high magnetic field applications, there exists a lack of domain images due to the difficulty in creating an efficient imaging system in such environments. Here, we present an innovative MOI technique that separates the imaging optical path from the one illuminating the object. The techniquemore » is based on thermal light GI and exploits correlations between light beams to retrieve the image of magnetic domains. As a proof of principle, the proposed technique is applied to the Faraday magneto-optical observation of the remanence domain structure of an yttrium iron garnet sample.« less

Single photon energy dispersive x-ray diffraction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Higginbotham, Andrew; Patel, Shamim; Ciricosta, Orlando

2014-03-15

With the pressure range accessible to laser driven compression experiments on solid material rising rapidly, new challenges in the diagnosis of samples in harsh laser environments are emerging. When driving to TPa pressures (conditions highly relevant to planetary interiors), traditional x-ray diffraction techniques are plagued by increased sources of background and noise, as well as a potential reduction in signal. In this paper we present a new diffraction diagnostic designed to record x-ray diffraction in low signal-to-noise environments. By utilising single photon counting techniques we demonstrate the ability to record diffraction patterns on nanosecond timescales, and subsequently separate, photon-by-photon, signalmore » from background. In doing this, we mitigate many of the issues surrounding the use of high intensity lasers to drive samples to extremes of pressure, allowing for structural information to be obtained in a regime which is currently largely unexplored.« less

Extreme Adiabatic Expansion in Micro-gravity: Modeling for the Cold Atomic Laboratory

NASA Astrophysics Data System (ADS)

Sackett, C. A.; Lam, T. C.; Stickney, J. C.; Burke, J. H.

2017-12-01

The upcoming Cold Atom Laboratory mission for the International Space Station will allow the investigation of ultracold gases in a microgravity environment. Cold atomic samples will be produced using evaporative cooling in a magnetic chip trap. We investigate here the possibility to release atoms from the trap via adiabatic expansion. We discuss both general considerations and a detailed model of the planned apparatus. We find that it should be possible to reduce the mean trap confinement frequency to about 0.2 Hz, which will correspond to a three-dimensional sample temperature of about 150 pK and a mean atom velocity of 0.1 mm/s.

Extreme Adiabatic Expansion in Micro-gravity: Modeling for the Cold Atomic Laboratory

NASA Astrophysics Data System (ADS)

Sackett, C. A.; Lam, T. C.; Stickney, J. C.; Burke, J. H.

2018-05-01

The upcoming Cold Atom Laboratory mission for the International Space Station will allow the investigation of ultracold gases in a microgravity environment. Cold atomic samples will be produced using evaporative cooling in a magnetic chip trap. We investigate here the possibility to release atoms from the trap via adiabatic expansion. We discuss both general considerations and a detailed model of the planned apparatus. We find that it should be possible to reduce the mean trap confinement frequency to about 0.2 Hz, which will correspond to a three-dimensional sample temperature of about 150 pK and a mean atom velocity of 0.1 mm/s.

Polonium 210Po activities in human blood of patients with ischaemic heart disease from Gdańsk in Poland.

PubMed

Boryło, Alicja; Skwarzec, Bogdan; Romańczyk, Grzegorz; Siebert, Janusz

The determination of polonium 210 Po in human blood samples is presented and discussed in this paper. The human blood samples were collected from patients of Medical University of Gdańsk with ischaemic heart disease ( morbus ischaemicus cordis , MIC ). The polonium concentrations in analyzed human blood samples are very differentiated. 210 Po is of particular interest in public health and although is present in the environment in extremely low amounts, it is easily bioaccumulated to the human body. The study shows that the amount of 210 Po that is incorporated into the human body depends on the food habits and some difference in its levels could be observed between smokers and non-smokers.

Corrosion Preventive Compounds Lifetime Testing

NASA Technical Reports Server (NTRS)

Hale, Stephanie M.; Kammerer, Catherine C.; Copp, Tracy L.

2007-01-01

Lifetime Testing of Corrosion Preventive Compounds (CPCs) was performed to quantify performance in the various environments to which the Space Shuttle Orbiter is exposed during a flight cycle. Three CPCs are approved for use on the Orbiter: RD Calcium Grease, Dinitrol AV-30, and Braycote 601 EF. These CPCs have been rigorously tested to prove that they mitigate corrosion in typical environments, but little information is available on how they perform in the unique combination of the coastal environment at the launch pad, the vacuum of low-earth orbit, and the extreme heat of reentry. Currently, there is no lifetime or reapplication schedule established for these compounds that is based on this combination of environmental conditions. Aluminum 2024 coupons were coated with the three CPCs and exposed to conditions that simulate the environments to which the Orbiter is exposed. Uncoated Aluminum 2024 coupons were exposed to the environmental conditions as a control. Visual inspection and Electro- Impedance Spectroscopy (EIS) were performed on the samples in order to determine the effectiveness of the CPCs. The samples were processed through five mission life cycles or until the visual inspection revealed the initiation of corrosion and EIS indicated severe degradation of the coating.

Corrosion Preventive Compounds Lifetime Testing

NASA Technical Reports Server (NTRS)

Hale, Stephanie M.; Kammerer, Catherine C.

2007-01-01

Lifetime Testing of Corrosion Preventive Compounds (CPCs) was performed to quantify performance in the various environments to which the Space Shuttle Orbiter is exposed during a flight cycle. Three CPCs are approved for use on the Orbiter: HD Calcium Grease, Dinitrol AV-30, and Braycote 601 EF. These CPCs have been rigorously tested to prove that they mitigate corrosion in typical environments, but little information is available on how they perform in the unique combination of the coastal environment at the launch pad, the vacuum of low-earth orbit, and the extreme heat of reentry. Currently, there is no lifetime or reapplication schedule established for these compounds that is based on this combination of environmental conditions. Aluminum 2024 coupons were coated with the three CPCs and exposed to conditions that simulate the environments to which the Orbiter is exposed. Uncoated Aluminum 2024 coupons were exposed to the environmental conditions as a control. Visual inspection and Electro- Impedance Spectroscopy (EIS) were performed on the samples in order to determine the effectiveness of the CPCs. The samples were processed through five mission life cycles or until the visual inspection revealed the initiation of corrosion and EIS indicated severe degradation of the coating.

Astrobiology as a tool for getting high school students interested in science

NASA Astrophysics Data System (ADS)

Van der Meer, B. W.; Alletto, James J.; Bryant, Dudley; Carini, Mike; Elliott, Larry; Gelderman, Richard; Mason, Wayne; McDaniel, Kerrie; McGruder, Charles H.; Rinehart, Claire; Tyler, Rico; Walker, Linda

2000-12-01

A workshop was held (10/99) for high school students and teachers on astrobiology. NASA provided support through an IDEAS grant. Out of 63 qualified applicants, 29 were accepted: 22 students (11 minorities) and 7 teachers. The worship was held on 2 successive weekends. Activities included: culturing microbes from human skin, discussing 'what is life?', building and using a 2-inch refractive telescope and a van-Leeuwenhoek- type microscope (each participant built and kept them), listening to lectures by Dr. Richard Gelderman on detecting extra solar planets and by Dr. Richard Hoover on life in extreme environments. Other activities included: collecting samples and isolating micro-organisms from the lost river cave, studying microbial life from extreme environments in the laboratory, using the internet as a research tool and debating the logistics and feasibility of a lunar colony. Written evaluations of the workshop led to the following conclusions: 48% of the students considered a possible career in the biological and/or astrophysical sciences, and half of these stated they were spurred on by the workshop itself.

Diversity of Ktedonobacteria with Actinomycetes-Like Morphology in Terrestrial Environments

PubMed Central

Yabe, Shuhei; Sakai, Yasuteru; Abe, Keietsu; Yokota, Akira

2017-01-01

Bacteria with an actinomycetes-like morphology have recently been discovered, and the class Ktedonobacteria was created for these bacteria in the phylum Chloroflexi. They may prove to be a valuable resource with the potential to produce unprecedented secondary metabolites. However, our understanding of their diversity, richness, habitat, and ecological significance is very limited. We herein developed a 16S rRNA gene-targeted, Ktedonobacteria-specific primer and analyzed ktedonobacterial amplicons. We investigated abundance, diversity, and community structure in forest and garden soils, sand, bark, geothermal sediment, and compost. Forest soils had the highest diversity among the samples tested (1181–2934 operational taxonomic units [OTUs]; Chao 1 estimate, 2503–5613; Shannon index, 4.21–6.42). A phylogenetic analysis of representative OTUs revealed at least eight groups within unclassified Ktedonobacterales, expanding the known diversity of this order. Ktedonobacterial communities markedly varied among our samples. The common mesic environments (soil, sand, and bark) were dominated by diverse phylotypes within the eight groups. In contrast, compost and geothermal sediment samples were dominated by known ktedonobacterial families (Thermosporotrichaceae and Thermogemmatisporaceae, respectively). The relative abundance of Ktedonobacteria in the communities, based on universal primers, was ≤0.8%, but was 12.9% in the geothermal sediment. These results suggest that unknown diverse Ktedonobacteria inhabit common environments including forests, gardens, and sand at low abundances, as well as extreme environments such as geothermal areas. PMID:28321007

A Submersible, Off-Axis Holographic Microscope for Detection of Microbial Motility and Morphology in Aqueous and Icy Environments

PubMed Central

Lindensmith, Christian A.; Rider, Stephanie; Bedrossian, Manuel; Wallace, J. Kent; Serabyn, Eugene; Showalter, G. Max; Deming, Jody W.; Nadeau, Jay L.

2016-01-01

Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to liquid water below in order to detect life. We have developed a submersible digital holographic microscope (DHM) that is capable of resolving individual bacterial cells, and demonstrated its utility for immediately imaging samples taken directly from sea ice at several locations near Nuuk, Greenland. In all samples, the appearance and motility of eukaryotes were conclusive signs of life. The appearance of prokaryotic cells alone was not sufficient to confirm life, but when prokaryotic motility occurred, it was rapid and conclusive. Warming the samples to above-freezing temperatures or supplementing with serine increased the number of motile cells and the speed of motility; supplementing with serine also stimulated chemotaxis. These results show that DHM is a useful technique for detection of active organisms in extreme environments, and that motility may be used as a biosignature in the liquid brines that persist in ice. These findings have important implications for the design of missions to icy environments and suggest ways in which DHM imaging may be integrated with chemical life-detection suites in order to create more conclusive life detection packages. PMID:26812683

A Submersible, Off-Axis Holographic Microscope for Detection of Microbial Motility and Morphology in Aqueous and Icy Environments.

PubMed

Lindensmith, Christian A; Rider, Stephanie; Bedrossian, Manuel; Wallace, J Kent; Serabyn, Eugene; Showalter, G Max; Deming, Jody W; Nadeau, Jay L

2016-01-01

Sea ice is an analog environment for several of astrobiology's near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to liquid water below in order to detect life. We have developed a submersible digital holographic microscope (DHM) that is capable of resolving individual bacterial cells, and demonstrated its utility for immediately imaging samples taken directly from sea ice at several locations near Nuuk, Greenland. In all samples, the appearance and motility of eukaryotes were conclusive signs of life. The appearance of prokaryotic cells alone was not sufficient to confirm life, but when prokaryotic motility occurred, it was rapid and conclusive. Warming the samples to above-freezing temperatures or supplementing with serine increased the number of motile cells and the speed of motility; supplementing with serine also stimulated chemotaxis. These results show that DHM is a useful technique for detection of active organisms in extreme environments, and that motility may be used as a biosignature in the liquid brines that persist in ice. These findings have important implications for the design of missions to icy environments and suggest ways in which DHM imaging may be integrated with chemical life-detection suites in order to create more conclusive life detection packages.

Automation Rover for Extreme Environments

NASA Technical Reports Server (NTRS)

Sauder, Jonathan; Hilgemann, Evan; Johnson, Michael; Parness, Aaron; Hall, Jeffrey; Kawata, Jessie; Stack, Kathryn

2017-01-01

Almost 2,300 years ago the ancient Greeks built the Antikythera automaton. This purely mechanical computer accurately predicted past and future astronomical events long before electronics existed1. Automata have been credibly used for hundreds of years as computers, art pieces, and clocks. However, in the past several decades automata have become less popular as the capabilities of electronics increased, leaving them an unexplored solution for robotic spacecraft. The Automaton Rover for Extreme Environments (AREE) proposes an exciting paradigm shift from electronics to a fully mechanical system, enabling longitudinal exploration of the most extreme environments within the solar system.

Metal mobilization by iron- and sulfur-oxidizing bacteria in a multiple extreme mine tailings in the Atacama Desert, Chile.

PubMed

Korehi, H; Blöthe, M; Sitnikova, M A; Dold, B; Schippers, A

2013-03-05

The marine shore sulfidic mine tailings dump at the Chañaral Bay in the Atacama Desert, northern Chile, is characterized by extreme acidity, high salinity, and high heavy metals concentrations. Due to pyrite oxidation, metals (especially copper) are mobilized under acidic conditions and transported toward the tailings surface and precipitate as secondary minerals (Dold, Environ. Sci. Technol. 2006, 40, 752-758.). Depth profiles of total cell counts in this almost organic-carbon free multiple extreme environment showed variable numbers with up to 10(8) cells g(-1) dry weight for 50 samples at four sites. Real-time PCR quantification and bacterial 16S rRNA gene diversity analysis via clone libraries revealed a dominance of Bacteria over Archaea and the frequent occurrence of the acidophilic iron(II)- and sulfur-oxidizing and iron(III)-reducing genera Acidithiobacillus, Alicyclobacillus, and Sulfobacillus. Acidophilic chemolithoautotrophic iron(II)-oxidizing bacteria were also frequently found via most-probable-number (MPN) cultivation. Halotolerant iron(II)-oxidizers in enrichment cultures were active at NaCl concentrations up to 1 M. Maximal microcalorimetrically determined pyrite oxidation rates coincided with maxima of the pyrite content, total cell counts, and MPN of iron(II)-oxidizers. These findings indicate that microbial pyrite oxidation and metal mobilization preferentially occur in distinct tailings layers at high salinity. Microorganisms for biomining with seawater salt concentrations obviously exist in nature.

Evaluating the Large-Scale Environment of Extreme Events Using Reanalyses

NASA Astrophysics Data System (ADS)

Bosilovich, M. G.; Schubert, S. D.; Koster, R. D.; da Silva, A. M., Jr.; Eichmann, A.

2014-12-01

Extreme conditions and events have always been a long standing concern in weather forecasting and national security. While some evidence indicates extreme weather will increase in global change scenarios, extremes are often related to the large scale atmospheric circulation, but also occurring infrequently. Reanalyses assimilate substantial amounts of weather data and a primary strength of reanalysis data is the representation of the large-scale atmospheric environment. In this effort, we link the occurrences of extreme events or climate indicators to the underlying regional and global weather patterns. Now, with greater than 3o years of data, reanalyses can include multiple cases of extreme events, and thereby identify commonality among the weather to better characterize the large-scale to global environment linked to the indicator or extreme event. Since these features are certainly regionally dependent, and also, the indicators of climate are continually being developed, we outline various methods to analyze the reanalysis data and the development of tools to support regional evaluation of the data. Here, we provide some examples of both individual case studies and composite studies of similar events. For example, we will compare the large scale environment for Northeastern US extreme precipitation with that of highest mean precipitation seasons. Likewise, southerly winds can shown to be a major contributor to very warm days in the Northeast winter. While most of our development has involved NASA's MERRA reanalysis, we are also looking forward to MERRA-2 which includes several new features that greatly improve the representation of weather and climate, especially for the regions and sectors involved in the National Climate Assessment.

Biofilms and planktonic cells of Deinococcus geothermalis in extreme environments

NASA Astrophysics Data System (ADS)

Panitz, Corinna; Reitz, Guenther; Rabbow, Elke; Rettberg, Petra; Flemming, Hans-Curt; Wingender, Jost; Froesler, Jan

In addition to the several extreme environments on Earth, Space can be considered as just another exceptional environment with a unique mixture of stress factors comprising UV radiation, vacuum, desiccation, temperature, ionizing radiation and microgravity. Life that processes in these environments can depend on the life forms and their state of living. The question is whether there are different strategies for individual microorganisms compared to communities of the same organisms to cope with the different factors of their surroundings. Comparative studies of the survi-val of these communities called biofilms and planktonic cell samples of Deinococcus geothermalis stand at the focal point of the presented investigations. A biofilm is a structured community of microorganisms that live encapsulated in a matrix of extracellular polymeric substances on a surface. Microorganisms living in a biofilm usually have significantly different properties to cooperate than individually living microorganisms of the same species. An advantage of the biofilm is increased resistance to various chemical and physical effects, while the dense extracellular matrix and the outer layer of the cells protect the interior of the microbial consortium. The space experiment BOSS (Biofilm organisms surfing Space) as part the ESA experimental unit EXPOSE R-2 with a planned launch date in July 2014 will be subsequently mounted on the Russian Svesda module outside the ISS. An international team of scientists coordinated by Dr. P. Rettberg will investigate the hypothesis whether microorganisms organized as biofilm outmatch the same microorganisms exposed individually in the long-term survival of the harsh environmental conditions as they occur in space and on Mars. Another protective function in the samples could be dust par-ticles for instance Mars regolith simulant contained inside the biofilms or mixed with the planktonic cells, as additional shelter especially against the extraterrestrial UV radiation. D. geothermalis besides others, like co-cultures of Halomonas muralis and Halococcus morrhuae, Bacillus horneckiae, Chroococcidiopsis CCMEE 029 and Streptomyces + Polaromonas and Arthrobacter strains from volcanic rocks, was involved in the several preparatory test runs at the Planetary and Space Simulation facilities at the German Aerospace Center in Cologne. Results of the already carried out EVTs (Experiment Verification Test) and the SVT (Science verification test) as EXPOSE-R2 mission pre-paration tests, where investigated parameters like dehydration, temperature extremes, extraterrestrial UV radiation, simulated Martian atmosphere, and a Mars-like UV climate were tested individually as well as in combination will be presented. Following exposure to the parameters listed above, the survival of both biofilms and planktonic cells of D. geothermalis was assessed in terms of (i) culturability by colony counts on R2A medium, (ii) membrane integrity by using the Live/Dead differential staining kit, (iii) ATP content by using a commercial luminometric assay, and (iv) the presence of 16S rRNA by fluorescence in situ hybridization. So far, the results suggest that Deinococcus geothermalis remains viable in the desiccated state over weeks to months, whereas culturability, intracellular ATP levels, and membrane integrity were preserved in biofilm cells at a significantly higher level than in planktonic cells. Furthermore, cells of both sample types were able to survive simulated space and Martian conditions and showed high resistance after irradiation with monochromatic and polychromatic UV. The results will contribute to the fundamental understanding of the opportunities and limitations of viability of microorganisms organized in biofilms or as planktonic cells under the extreme environ-mental conditions of space or other planets.

Small-scale studies of roasted ore waste reveal extreme ranges of stable mercury isotope signatures

NASA Astrophysics Data System (ADS)

Smith, Robin S.; Wiederhold, Jan G.; Jew, Adam D.; Brown, Gordon E.; Bourdon, Bernard; Kretzschmar, Ruben

2014-07-01

Active and closed Hg mines are significant sources of Hg contamination to the environment, mainly due to large volumes of mine waste material disposed of on-site. The application of Hg isotopes as source tracer from such contaminated sites requires knowledge of the Hg isotope signatures of different materials potentially released to the environment. Previous work has shown that calcine, the waste residue of the on-site ore roasting process, can exhibit distinct Hg isotope signatures compared with the primary ore. Here, we report results from a detailed small-scale study of Hg isotope variations in calcine collected from the closed New Idria Hg mine, San Benito County, CA, USA. The calcine samples exhibited different internal layering features which were investigated using optical microscopy, micro X-ray fluorescence, micro X-ray absorption spectroscopy (μ-XAS), and stable Hg isotope analysis. Significant Fe, S, and Hg concentration gradients were found across the different internal layers. Isotopic analyses revealed an extreme variation with pronounced isotopic gradients across the internal layered features. Overall, δ202Hg (±0.10‰, 2 SD) describing mass-dependent fractionation (MDF) ranged from -5.96 to 14.49‰, which is by far the largest range of δ202Hg values reported for any environmental sample. In addition, Δ199Hg (±0.06‰, 2 SD) describing mass-independent fractionation (MIF) ranged from -0.17 to 0.21‰. The μ-XAS analyses suggested that cinnabar and metacinnabar are the dominant Hg-bearing phases in the calcine. Our results demonstrate that the incomplete roasting of HgS ores in Hg mines can cause extreme mass-dependent Hg isotope fractionations at the scale of individual calcine pieces with enrichments in both light and heavy Hg isotopes relative to the primary ore signatures. This finding has important implications for the application of Hg isotopes as potential source tracers for Hg released to the environment from closed Hg mines and highlights the need for detailed source signature identification.

Moving in extreme environments: what's extreme and who decides?

PubMed

Cotter, James David; Tipton, Michael J

2014-01-01

Humans work, rest and play in immensely varied extreme environments. The term 'extreme' typically refers to insufficiency or excess of one or more stressors, such as thermal energy or gravity. Individuals' behavioural and physiological capacity to endure and enjoy such environments varies immensely. Adverse effects of acute exposure to these environments are readily identifiable (e.g. heat stroke or bone fracture), whereas adverse effects of chronic exposure (e.g. stress fractures or osteoporosis) may be as important but much less discernable. Modern societies have increasingly sought to protect people from such stressors and, in that way, minimise their adverse effects. Regulations are thus established, and advice is provided on what is 'acceptable' exposure. Examples include work/rest cycles in the heat, hydration regimes, rates of ascent to and duration of stay at altitude and diving depth. While usually valuable and well intentioned, it is important to realise the breadth and importance of limitations associated with such guidelines. Regulations and advisories leave less room for self-determination, learning and perhaps adaptation. Regulations based on stress (e.g. work/rest cycles relative to WBGT) are more practical but less direct than those based on strain (e.g. core temperature), but even the latter can be substantively limited (e.g. by lack of criterion validation and allowance for behavioural regulation in the research on which they are based). Extreme Physiology & Medicine is publishing a series of reviews aimed at critically examining the issues involved with self- versus regulation-controlled human movement acutely and chronically in extreme environments. These papers, arising from a research symposium in 2013, are about the impact of people engaging in such environments and the effect of rules and guidelines on their safety, enjoyment, autonomy and productivity. The reviews will cover occupational heat stress, sporting heat stress, hydration, diving, extreme loading, chronic unloading and high altitude. Ramifications include factors such as health and safety, productivity, enjoyment and autonomy, acute and chronic protection and optimising adaptation.

Evaluation of the Human/Extreme Environment Interaction: Implications for Enhancing Operational Performance and Recovery

DTIC Science & Technology

2012-10-01

saturation was evaluated before and after exercise, and during recovery at 0:30, 2:30, 4:30, and 6:00 using a pulse oximeter (Nonin Onyx II 9550, Plymouth...Each collection was collected for 5 min, with the last 3 min averaged to represent the sample period. Pulse Oximetry. Blood oxygen ...would lead to more ATP per molecule of oxygen . The paradox with short-term and long-term metabolic adaptations is yet to be completely understood

Field Exploration and Life Detection Sampling for Planetary Analogue Research (FELDSPAR): Variability and Correlation in Biomarker and Mineralogy Measurements from Icelandic Mars Analogues

NASA Technical Reports Server (NTRS)

Gentry, D.; Amador, E.; Cable, M. L.; Cantrell, T.; Chaudry, N.; Cullen, T.; Duca, Z.; Jacobsen, M.; Kirby, J.; McCaig, H.;

Finding and identifying the viral needle in the metagenomic haystack: trends and challenges

PubMed Central

Soueidan, Hayssam; Schmitt, Louise-Amélie; Candresse, Thierry; Nikolski, Macha

2015-01-01

Collectively, viruses have the greatest genetic diversity on Earth, occupy extremely varied niches and are likely able to infect all living organisms. Viral infections are an important issue for human health and cause considerable economic losses when agriculturally important crops or husbandry animals are infected. The advent of metagenomics has provided a precious tool to study viruses by sampling them in natural environments and identifying the genomic composition of a sample. However, reaching a clear recognition and taxonomic assignment of the identified viruses has been hampered by the computational difficulty of these problems. In this perspective paper we examine the trends in current research for the identification of viral sequences in a metagenomic sample, pinpoint the intrinsic computational difficulties for the identification of novel viral sequences within metagenomic samples, and suggest possible avenues to overcome them. PMID:25610431

Radiation and Thermal Cycling Effects on EPC1001 Gallium Nitride Power Transistors

NASA Technical Reports Server (NTRS)

Patterson, Richard L.; Scheick, Leif Z.; Lauenstein, Jean M.; Casey, Megan C.; Hammoud, Ahmad

2012-01-01

Electronics designed for use in NASA space missions are required to work efficiently and reliably under harsh environment conditions. These include radiation, extreme temperatures, and thermal cycling, to name a few. Information pertaining to performance of electronic parts and systems under hostile environments is very scarce, especially for new devices. Such data is very critical so that proper design is implemented in order to ensure mission success and to mitigate risks associated with exposure of on-board systems to the operational environment. In this work, newly-developed enhancement-mode field effect transistors (FET) based on gallium nitride (GaN) technology were exposed to various particles of ionizing radiation and to long-term thermal cycling over a wide temperature range. Data obtained on control (un-irradiated) and irradiated samples of these power transistors are presented and the results are discussed.

Hardness assurance testing and radiation hardening by design techniques for silicon-germanium heterojunction bipolar transistors and digital logic circuits

NASA Astrophysics Data System (ADS)

Sutton, Akil K.

Hydrocarbon exploration, global navigation satellite systems, computed tomography, and aircraft avionics are just a few examples of applications that require system operation at an ambient temperature, pressure, or radiation level outside the range covered by military specifications. The electronics employed in these applications are known as "extreme environment electronics." On account of the increased cost resulting from both process modifications and the use of exotic substrate materials, only a handful of semiconductor foundries have specialized in the production of extreme environment electronics. Protection of these electronic systems in an extreme environment may be attained by encapsulating sensitive circuits in a controlled environment, which provides isolation from the hostile ambient, often at a significant cost and performance penalty. In a significant departure from this traditional approach, system designers have begun to use commercial off-the-shelf technology platforms with built in mitigation techniques for extreme environment applications. Such an approach simultaneously leverages the state of the art in technology performance with significant savings in project cost. Silicon-germanium is one such commercial technology platform that demonstrates potential for deployment into extreme environment applications as a result of its excellent performance at cryogenic temperatures, remarkable tolerance to radiation-induced degradation, and monolithic integration with silicon-based manufacturing. In this dissertation the radiation response of silicon-germanium technology is investigated, and novel transistor-level layout-based techniques are implemented to improve the radiation tolerance of HBT digital logic.

Prevention through policy: Urban macroplastic leakages to the marine environment during extreme rainfall events.

PubMed

Axelsson, Charles; van Sebille, Erik

2017-11-15

The leakage of large plastic litter (macroplastics) into the ocean is a major environmental problem. A significant fraction of this leakage originates from coastal cities, particularly during extreme rainfall events. As coastal cities continue to grow, finding ways to reduce this macroplastic leakage is extremely pertinent. Here, we explore why and how coastal cities can reduce macroplastic leakages during extreme rainfall events. Using nine global cities as a basis, we establish that while cities actively create policies that reduce plastic leakages, more needs to be done. Nonetheless, these policies are economically, socially and environmentally cobeneficial to the city environment. While the lack of political engagement and economic concerns limit these policies, lacking social motivation and engagement is the largest limitation towards implementing policy. We recommend cities to incentivize citizen and municipal engagement with responsible usage of plastics, cleaning the environment and preparing for future extreme rainfall events. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Mercury and gold concentrations of highly polluted environmental samples determined using prompt gamma-ray analysis and instrument neutron activation analysis

NASA Astrophysics Data System (ADS)

Osawa, Takahito; Hatsukawa, Yuichi; Appel, Peter W. U.; Matsue, Hideaki

2011-04-01

The authors have established a method of determining mercury and gold in severely polluted environmental samples using prompt gamma-ray analysis (PGA) and instrumental neutron activation analysis (INAA). Since large amounts of mercury are constantly being released into the environment by small-scale gold mining in many developing countries, the mercury concentration in tailings and water has to be determined to mitigate environmental pollution. Cold-vapor atomic absorption analysis, the most pervasive method of mercury analysis, is not suitable because tailings and water around mining facilities have extremely high mercury concentrations. On the other hand, PGA can determine high mercury concentrations in polluted samples as it has an appropriate level of sensitivity. Moreover, gold concentrations can be determined sequentially by using INAA after PGA. In conclusion, the analytical procedure established in this work using PGA and INAA is the best way to evaluate the degree of pollution and the tailing resource value. This method will significantly contribute to mitigating problems in the global environment.

In vacuo X-ray data collection from graphene-wrapped protein crystals.

PubMed

Warren, Anna J; Crawshaw, Adam D; Trincao, Jose; Aller, Pierre; Alcock, Simon; Nistea, Ioana; Salgado, Paula S; Evans, Gwyndaf

2015-10-01

The measurement of diffraction data from macromolecular crystal samples held in vacuo holds the promise of a very low X-ray background and zero absorption of incident and scattered beams, leading to better data and the potential for accessing very long X-ray wavelengths (>3 Å) for native sulfur phasing. Maintaining the hydration of protein crystals under vacuum is achieved by the use of liquid jets, as with serial data collection at free-electron lasers, or is side-stepped by cryocooling the samples, as implemented at new synchrotron beamlines. Graphene has been shown to protect crystals from dehydration by creating an extremely thin layer that is impermeable to any exchanges with the environment. Furthermore, owing to its hydrophobicity, most of the aqueous solution surrounding the crystal is excluded during sample preparation, thus eliminating most of the background caused by liquid. Here, it is shown that high-quality data can be recorded at room temperature from graphene-wrapped protein crystals in a rough vacuum. Furthermore, it was observed that graphene protects crystals exposed to different relative humidities and a chemically harsh environment.

Cretaceous shales from the western interior of North America: sulfur/carbon ratios and sulfur-isotope composition.

USGS Publications Warehouse

Gautier, D.L.

1986-01-01

Sulphur/carbon ratios in cores of selected Cretaceous marine shales average 0.67, a value greater than that observed in recent marine sediments and much higher than global values calculated for the Cretaceous. This may be ascribed to generally low levels of bioturbation and enhanced efficiency of sulphate reduction due to low oxygen levels in Cretaceous seaways. Isotopic compositions of pyrite sulphur vary systematically with level of oxygenation of the depositional environment and therefore with organic carbon abundance and type of organic matter. Samples with >4% organic carbon are extremely depleted in 34S (mean delta 34S -31per mille) and contain hydrogen-rich organic matter. Samples containing <1.5% organic carbon display relatively 'heavy' but wide-ranging delta 34S values (-34.6 to +16.8per mille) and contain hydrogen-poor organic matter. Samples with intermediate amounts of organic carbon have average delta 34S of -25.9per mille and contain both types of organic matter. Relations between the nature of these shales, and their sedimentation rate and depositional environment are discussed.-L.C.H.

Compact turnkey focussing neutron guide system for inelastic scattering investigations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brandl, G., E-mail: g.brandl@fz-juelich.de; Georgii, R.; Dunsiger, S. R.

2015-12-21

We demonstrate the performance of a compact neutron guide module which boosts the intensity in inelastic neutron scattering experiments by approximately a factor of 40. The module consists of two housings containing truly curved elliptic focussing guide elements, positioned before and after the sample. The advantage of the module lies in the ease with which it may be reproducibly mounted on a spectrometer within a few hours, on the same timescale as conventional sample environments. It is particularly well suited for samples with a volume of a few mm{sup 3}, thus enabling the investigation of materials which to date wouldmore » have been considered prohibitively small or samples exposed to extreme environments, where there are space constraints. We benchmark the excellent performance of the module by measurements of the structural and magnetic excitations in single crystals of model systems. In particular, we report the phonon dispersion in the simple element lead. We also determine the magnon dispersion in the spinel ZnCr{sub 2}Se{sub 4} (V = 12.5 mm{sup 3}), where strong magnetic diffuse scattering at low temperatures evolves into distinct helical order.« less

Extreme Environments Development of Decision Processes and Training Programs for Medical Policy Formulation

NASA Technical Reports Server (NTRS)

Stough, Roger

2004-01-01

The purpose of this workshop was to survey existing health and safety policies as well as processes and practices for various extreme environments; to identify strengths and shortcomings of these processes; and to recommend parameters for inclusion in a generic approach to policy formulation, applicable to the broadest categories of extreme environments. It was anticipated that two additional workshops would follow. The November 7, 2003 workshop would be devoted to the evaluation of different model(s) and a concluding expert evaluation of the usefulness of the model using a policy formulation example. The final workshop was planned for March 2004.

The western Qaidam Basin as a potential Martian environmental analogue: An overview

NASA Astrophysics Data System (ADS)

Anglés, Angélica; Li, Yiliang

2017-05-01

The early Martian environment is interpreted as warmer and wetter, before a significant change in its global climatic conditions irreversibly led to the current hyperarid environments. This transition is one of the most intriguing processes of Martian history. The extreme climatic change is preserved in the salt deposits, desiccated landscapes, and geomorphological structures that were shaped by the evaporation of water. However, until a manned journey to Mars is feasible, many Martian materials, morphological structures, and much of its evolutionary history will continue to be poorly understood. In this regard, searching and investigating Martian analogues are still meaningful. To find an Earth environment with a whole set of Martian structures distributed at a scale comparable to Mars is even more important to test landing crafts and provide optimized working parameters for rovers. The western Qaidam Basin in North Tibetan Plateau is such a Martian analogue. The area harbors one of the most extreme hyperarid environments on Earth and contains a series of ancient lakes that evaporated at different evolutionary stages during the rise of the Tibetan Plateau. Large quantities of salts and geomorphological features formed during the transition of warmer-and-wet to colder-and-dry conditions provide unique references to study the modern Martian surface and interpret the orbital data. We present numerous similarities and results of investigations that suggest the Qaidam Basin as a potential analogue to study modern geomorphic processes on Mars, and suggest that this is an essential site to test future Mars sample return missions.

An Automaton Rover for Extreme Environments: Rethinking an Approach to Surface Mobility

NASA Astrophysics Data System (ADS)

Sauder, J.; Hilgemman, E.; Stack, K.; Kawata, J.; Parness, A.; Johnson, M.

2017-11-01

An Automaton Rover for Extreme Environments (AREE) enables long duration in-situ mobility on the surface of Venus through a simplified design and robust mechanisms. The goal is to design a rover capable of operating for months on the surface of Venus.

Performance of Waterless Concrete

NASA Technical Reports Server (NTRS)

Toutanji, Houssam; Evans, Steve; Grugel, Richard N.

2010-01-01

The development of permanent lunar bases is constrained by performance of construction materials and availability of in-situ resources. Concrete seems a suitable construction material for the lunar environment, but water, one of its major components, is an extremely scarce resource on the Moon. This study explores an alternative to hydraulic concrete by replacing the binding mix of concrete (cement and water) with sulfur. Sulfur is a volatile element on the lunar surface that can be extracted from lunar soils by heating. Sulfur concrete mixes were prepared to investigate the effect of extreme environmental conditions on the properties of sulfur concrete. A hypervelocity impact test was conducted, having as its target a 5-cm cubic sample of sulfur concrete. This item consisted of JSC-1 lunar regolith simulant (65%) and sulfur (35%). The sample was placed in the MSFC Impact Test Facility s Micro Light Gas Gun target chamber, and was struck by a 1-mm diameter (1.4e-03 g) aluminum projectile at 5.85 km/s. In addition, HZTERN code, provided by NASA was used to study the effectiveness of sulfur concrete when subjected to space radiation.

Extreme Mean and Its Applications

NASA Technical Reports Server (NTRS)

Swaroop, R.; Brownlow, J. D.

1979-01-01

Extreme value statistics obtained from normally distributed data are considered. An extreme mean is defined as the mean of p-th probability truncated normal distribution. An unbiased estimate of this extreme mean and its large sample distribution are derived. The distribution of this estimate even for very large samples is found to be nonnormal. Further, as the sample size increases, the variance of the unbiased estimate converges to the Cramer-Rao lower bound. The computer program used to obtain the density and distribution functions of the standardized unbiased estimate, and the confidence intervals of the extreme mean for any data are included for ready application. An example is included to demonstrate the usefulness of extreme mean application.

40 CFR 355.12 - What quantities of extremely hazardous substances trigger emergency planning requirements?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 28 2011-07-01 2011-07-01 false What quantities of extremely hazardous substances trigger emergency planning requirements? 355.12 Section 355.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS...

Isolation and Characterization of Bacteria Capable of Tolerating the Extreme Conditions of Clean Room Environments▿

PubMed Central

La Duc, Myron T.; Dekas, Anne; Osman, Shariff; Moissl, Christine; Newcombe, David; Venkateswaran, Kasthuri

2007-01-01

In assessing the bacterial populations present in spacecraft assembly, spacecraft test, and launch preparation facilities, extremophilic bacteria (requiring severe conditions for growth) and extremotolerant bacteria (tolerant to extreme conditions) were isolated. Several cultivation approaches were employed to select for and identify bacteria that not only survive the nutrient-limiting conditions of clean room environments but can also withstand even more inhospitable environmental stresses. Due to their proximity to spacefaring objects, these bacteria pose a considerable risk for forward contamination of extraterrestrial sites. Samples collected from four geographically distinct National Aeronautics and Space Administration clean rooms were challenged with UV-C irradiation, 5% hydrogen peroxide, heat shock, pH extremes (pH 3.0 and 11.0), temperature extremes (4°C to 65°C), and hypersalinity (25% NaCl) prior to and/or during cultivation as a means of selecting for extremotolerant bacteria. Culture-independent approaches were employed to measure viable microbial (ATP-based) and total bacterial (quantitative PCR-based) burdens. Intracellular ATP concentrations suggested a viable microbial presence ranging from below detection limits to 106 cells/m2. However, only 0.1 to 55% of these viable cells were able to grow on defined culture medium. Isolated members of the Bacillaceae family were more physiologically diverse than those reported in previous studies, including thermophiles (Geobacillus), obligate anaerobes (Paenibacillus), and halotolerant, alkalophilic species (Oceanobacillus and Exiguobacterium). Non-spore-forming microbes (α- and β-proteobacteria and actinobacteria) exhibiting tolerance to the selected stresses were also encountered. The multiassay cultivation approach employed herein enhances the current understanding of the physiological diversity of bacteria housed in these clean rooms and leads us to ponder the origin and means of translocation of thermophiles, anaerobes, and halotolerant alkalophiles into these environments. PMID:17308177

Temperature histories of commercial flights at severe conditions from GASP data

NASA Technical Reports Server (NTRS)

Jasperson, W. H.; Nastrom, G. D.

1983-01-01

The thermal environment of commercial aircraft from a data set gathered during the Global Atmospheric Sampling Program (GASP) is studied. The data set covers a four-year period of measurements. The report presents plots of airplane location and speed and atmospheric temperature as functions of elapsed time for 35 extreme-condition flights, selected by minimum values of several temperature parameters. One of these parameters, the severity factor, is an approximation of the in-flight wing-tank temperature. Representative low-severity-factor flight histories may be useful for actual temperature-profile inputs to design and research studies. Comparison of the GASP atmospheric temperatures to interpolated temperatures from National Meteorological Center and Global Weather Central analysis fields shows that the analysis temperatures are slightly biased toward warmer than actual temperatures, particularly over oceans and at extreme conditions.

Israeli nurse practice environment characteristics, retention, and job satisfaction.

PubMed

Dekeyser Ganz, Freda; Toren, Orly

2014-02-24

There is an international nursing shortage. Improving the practice environment has been shown to be a successful strategy against this phenomenon, as the practice environment is associated with retention and job satisfaction. The Israeli nurse practice environment has not been measured. The purpose of this study was to measure practice environment characteristics, retention and job satisfaction and to evaluate the association between these variables. A demographic questionnaire, the Practice Environment Scale, and a Job Satisfaction Questionnaire were administered to Israeli acute and intensive care nurses working in 7 hospitals across the country. Retention was measured by intent to leave the organization and work experience. A convenience sample of registered nurses was obtained using a bi-phasic, stratified, cluster design. Data were collected based on the preferences of each unit, either distribution during various shifts or at staff meetings; or via staff mailboxes. Descriptive statistics were used to describe the sample and results of the questionnaires. Pearson Product Moment Correlations were used to determine significant associations among the variables. A multiple regression model was designed where the criterion variable was the practice environment. Analyses of variance determined differences between groups on nurse practice environment characteristics. 610 nurses reported moderate levels of practice environment characteristics, where the lowest scoring characteristic was 'appropriate staffing and resources'. Approximately 9% of the sample reported their intention to leave and the level of job satisfaction was high. A statistically significant, negative, weak correlation was found between intention to leave and practice environment characteristics, with a moderate correlation between job satisfaction and practice environment characteristics. 'Appropriate staffing and resources' was the only characteristic found to be statistically different based on hospital size and geographic region. This study supports the international nature of the vicious cycle that includes a poor quality practice environment, decreased job satisfaction and low nurse retention. Despite the extreme nursing shortage in Israel, perceptions of the practice environment were similar to other countries. Policy makers and hospital managers should address the practice environment, in order to improve job satisfaction and increase retention.

Prokaryotic diversity and community composition in the Salar de Uyuni, a large scale, chaotropic salt flat.

PubMed

dC Rubin, Sergio S; Marín, Irma; Gómez, Manuel J; Morales, Eduardo A; Zekker, Ivar; San Martín-Uriz, Patxi; Rodríguez, Nuria; Amils, Ricardo

2017-09-01

Salar de Uyuni (SdU), with a geological history that reflects 50 000 years of climate change, is the largest hypersaline salt flat on Earth and is estimated to be the biggest lithium reservoir in the world. Its salinity reaches saturation levels for NaCl, a kosmotropic salt, and high concentrations of MgCL 2 and LiCl, both salts considered important chaotrophic stressors. In addition, extreme temperatures, anoxic conditions, high UV irradiance, high albedo and extremely low concentrations of phosphorous, make SdU a unique natural extreme environment in which to contrast hypotheses about limiting factors of life diversification. Geophysical studies of brines from different sampling stations show that water activity is rather constant along SdU. Geochemical measurements show significant differences in magnesium concentration, ranging from 0.2 to 2M. This work analyses the prokaryotic diversity and community structure at four SdU sampling stations, selected according to their location and ionic composition. Prokaryotic communities were composed of both Archaea (with members of the classes Halobacteria, Thermoplasmata and Nanohaloarchaea, from the Euryarchaeota and Nanohaloarcheota phyla respectively) and Bacteria (mainly belonging to Bacteroidetes and Proteobacteria phyla). The important differences in composition of microbial communities inversely correlate with Mg 2+ concentration, suggesting that prokaryotic diversity at SdU is chaotropic dependent. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.

TransFormers for Ensuring Long-Term Operations in Lunar Extreme Environments

NASA Technical Reports Server (NTRS)

Mantovani, J. G.; Stoica, A.; Alkalai, L.; Wilcox, B.; Quadrelli, M.

2016-01-01

"Surviving Extreme Space Environments" (EE) is one of NASA's Space Technology Grand Challenges. Power generation and thermal control are the key survival ingredients that allow a robotic explorer to cope with the EE using resources available to it, for example, by harvesting the local solar energy or by utilizing an onboard radioisotope thermoelectric generator (RTG). TransFormers (TFs) are a new technology concept designed to transform a localized area within a harsh extreme environment into a survivable micro-environment by projecting energy to the precise location where robots or humans operate. For example, TFs placed at a location on the rim of Shackleton Crater, which is illuminated by solar radiation for most of the year, would be able to reflect solar energy onto robots operating in the dark cold crater. TFs utilize a shape transformation mechanism to un-fold from a compact volume to a large reflective surface, and to control how much-and where-the energy is projected, and by adjusting for the changing position of the sun. TFs would enable in-situ resource utilization (ISRU) activities within locations of high interest that would normally be unreachable because of their extreme environment

[The acclimatization to extreme environments and its physiological mechanisms].

PubMed

Wang, Hai; Liu, Wei; Yang, Dan-Feng; Zhao, Xiao-Ling; Long, Chao-Liang; Yin, Zhao-Yun; Liu, Jia-Ying

2012-11-01

Acclimatization is a process of biological adaptation when exposed to environmental factors such as hypoxia, cold and heat for prolonged periods of time, where non-genetical variations play a role in allowing subjects to tolerate hypoxic, cold or hot environments. This review focuses on the characteristics and mechanisms of acclimatization found through major research advances by our institute. First, the mechanisms underlying the acclimatization to extreme environments are complex. In our investigations, the physiological changes of multiple systems including the nervous, circulatory, respiratory, and hemopoietic system were demonstrated when the acclimatization to hypoxia was developed, and the underlying significance of hypoxia-inducible factor-1 (HIF-1) was investigated. Second, it is suggested that the development of acclimatization to extreme environments is complicated. Hypoxia and cold coexist at high altitude. Our investigations revealed the characteristics of negative cross-relationship in the acclimatization to hypoxia and cold. And third, it is interesting for us to understand that acclimatization to extreme environments is transferable among individuals, and the characteristics of heat acclimatization-inducing factor (HAlF) were presented. The above findings will provide a theoretical guidance for protective operations and help to establish a solid foundation for future research related to acclimatization.

Heatshield for Extreme Entry Environment Technology: Results from Acreage and Integrated Seams Arcjet Testing

NASA Technical Reports Server (NTRS)

Venkatapathy, Ethiraj

2016-01-01

This invited talk will give a brief overview of the integrated heat-shield system design that requires seams and the extreme environment conditions that HEEET should be demonstrated to be capable of thermal performance without fail. We have tested HEEET across many different facilities and at conditions that are extreme. The presentation will highlight the performance of both the acreage as well as integrated seam at these conditions. The Invite talks are 10 min and hence this presentation will be short.

Extreme Environments Capabilities at Glenn Research Center

NASA Technical Reports Server (NTRS)

Balcerski, Jeffrey; Kremic, Tibor; Arnett, Lori; Vento, Dan; Nakley, Leah

2016-01-01

The NASA Glenn Research Center has several facilities that can provide testing for extreme evironments of interest to the New Frontiers community. This includes the Glenn Extreme Enivironments Rig (GEER) which can duplicate the atmospheric chemistry and conditions for the Venus surface or any other planet with a hot environment. GRC also has several cryogenic facilities which have the capability to run with hydrogen atmospheres, hydrocarbon atmosphere, CO2 based atmospheres or nitrogen atmospheres. The cryogenic facilities have the capability to emulate Titan lakes.

Kinematic Clues to OB Field Star Origins: Radial Velocities, Runaways, and Binaries

NASA Astrophysics Data System (ADS)

Januszewski, Helen; Castro, Norberto; Oey, Sally; Becker, Juliette; Kratter, Kaitlin M.; Mateo, Mario; Simón-Díaz, Sergio; Bjorkman, Jon E.; Bjorkman, Karen; Sigut, Aaron; Smullen, Rachel; M2FS Team

2018-01-01

Field OB stars are a crucial probe of star formation in extreme conditions. Properties of massive stars formed in relative isolation can distinguish between competing star formation theories, while the statistics of runaway stars allow an indirect test of the densest conditions in clusters. To address these questions, we have obtained multi-epoch, spectroscopic observations for a spatially complete sample of 48 OB field stars in the SMC Wing with the IMACS and M2FS multi-object spectrographs at the Magellan Telescopes. The observations span 3-6 epochs per star, with sampling frequency ranging from one day to about one year. From these spectra, we have calculated the radial velocities (RVs) and, in particular, the systemic velocities for binaries. Thus, we present the intrinsic RV distribution largely uncontaminated by binary motions. We estimate the runaway frequency, corresponding to the high velocity stars in our sample, and we also constrain the binary frequency. The binary frequency and fitted orbital parameters also place important constraints on star formation theories, as these properties drive the process of runaway ejection in clusters, and we discuss these properties as derived from our sample. This unique kinematic analysis of a high mass field star population thus provides a new look at the processes governing formation and interaction of stars in environments at extreme densities, from isolation to dense clusters.

Impact of Total Ionizing Dose Radiation Testing and Long-Term Thermal Cycling on the Operation of CMF20120D Silicon Carbide Power MOSFET

NASA Technical Reports Server (NTRS)

Patterson, Richard L.; Scheidegger, Robert J.; Lauenstein, Jean-Marie; Casey, Megan; Scheick, Leif; Hammoud, Ahmad

2013-01-01

Power systems designed for use in NASA space missions are required to work reliably under harsh conditions including radiation, thermal cycling, and extreme temperature exposures. Silicon carbide devices show great promise for use in future power electronics systems, but information pertaining to performance of the devices in the space environment is very scarce. A silicon carbide N-channel enhancement-mode power MOSFET called the CMF20120 is of interest for use in space environments. Samples of the device were exposed to radiation followed by long-term thermal cycling to address their reliability for use in space applications. The results of the experimental work are presentd and discussed.

Using the Student's "t"-Test with Extremely Small Sample Sizes

ERIC Educational Resources Information Center

de Winter, J. C .F.

2013-01-01

Researchers occasionally have to work with an extremely small sample size, defined herein as "N" less than or equal to 5. Some methodologists have cautioned against using the "t"-test when the sample size is extremely small, whereas others have suggested that using the "t"-test is feasible in such a case. The present…

Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment.

PubMed

Glaring, Mikkel A; Vester, Jan K; Stougaard, Peter

2017-01-01

Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.

Evaluating teams in extreme environments: from issues to answers.

PubMed

Bishop, Sheryl L

2004-07-01

The challenge to effectively evaluating teams in extreme environments necessarily involves a wide range of physiological, psychological, and psychosocial factors. The high reliance on technology, the growing frequency of multinational and multicultural teams, and the demand for longer duration missions all further compound the complexity of the problem. The primary goal is the insurance of human health and well-being with expectations that such priorities will naturally lead to improved chances for performance and mission success. This paper provides an overview of some of the most salient immediate challenges for selecting, training, and supporting teams in extreme environments, gives exemplars of research findings concerning these challenges, and discusses the need for future research.

Optical Property Measurements on the Stardust Sample Return Capsule

NASA Technical Reports Server (NTRS)

Finckenor, Miria

2007-01-01

The Advanced Materials for Exploration (AME) task Materials Analysis of Returned Hardware from Stardust received funding to perform non-destructive analyses of the non-primary science hardware components of the Stardust sample return capsule. These components were (a) the blunt body reentry heatshield, encased in Phenolic Impregnated Carbon Ablator (PICA); (b) the backshell of Super Lightweight Ablator 561 (SLA-561) material handpacked into phenolic Flexcore and coated with CV-1100 silicone; (c) the rope seal used in between the heatshield and backshell; (d) the internal multi-layer insulation (MLI) blankets; and (e) parts of the Kevlar straps left attached to the backshell. These components were analyzed to determine the materials' durability in the space environment. The goals of the task were (a) to determine how the various materials from which the components were built weathered the extreme temperatures and harsh space environment during the capsule's nearly 7-year voyage to and from its rendezvous with Comet Wild 2 and (b) to provide lessons-learned data for designers of future missions.

NASA Extreme Environment Mission Operations: Science Operations Development for Human Exploration

NASA Technical Reports Server (NTRS)

Bell, Mary S.

2014-01-01

The purpose of NASA Extreme Environment Mission Operations (NEEMO) mission 16 in 2012 was to evaluate and compare the performance of a defined series of representative near-Earth asteroid (NEA) extravehicular activity (EVA) tasks under different conditions and combinations of work systems, constraints, and assumptions considered for future human NEA exploration missions. NEEMO 16 followed NASA's 2011 Desert Research and Technology Studies (D-RATS), the primary focus of which was understanding the implications of communication latency, crew size, and work system combinations with respect to scientific data quality, data management, crew workload, and crew/mission control interactions. The 1-g environment precluded meaningful evaluation of NEA EVA translation, worksite stabilization, sampling, or instrument deployment techniques. Thus, NEEMO missions were designed to provide an opportunity to perform a preliminary evaluation of these important factors for each of the conditions being considered. NEEMO 15 also took place in 2011 and provided a first look at many of the factors, but the mission was cut short due to a hurricane threat before all objectives were completed. ARES Directorate (KX) personnel consulted with JSC engineers to ensure that high-fidelity planetary science protocols were incorporated into NEEMO mission architectures. ARES has been collaborating with NEEMO mission planners since NEEMO 9 in 2006, successively building upon previous developments to refine science operations concepts within engineering constraints; it is expected to continue the collaboration as NASA's human exploration mission plans evolve.

Diversity of Heterotrophic Protists from Extremely Hypersaline Habitats.

PubMed

Park, Jong Soo; Simpson, Alastair G B

2015-09-01

Heterotrophic protists (protozoa) are a diverse but understudied component of the biota of extremely hypersaline environments, with few data on molecular diversity within halophile 'species', and almost nothing known of their biogeographic distribution. We have garnered SSU rRNA gene sequences for several clades of halophilic protozoa from enrichments from waters of >12.5% salinity from Australia, North America, and Europe (6 geographic sites, 25 distinct samples). The small stramenopile Halocafeteria was found at all sites, but phylogenies did not show clear geographic clustering. The ciliate Trimyema was recorded from 6 non-European samples. Phylogenies confirmed a monophyletic halophilic Trimyema group that included possible south-eastern Australian, Western Australian and North American clusters. Several halophilic Heterolobosea were detected, demonstrating that Pleurostomum contains at least three relatively distinct clades, and increasing known continental ranges for Tulamoeba peronaphora and Euplaesiobystra hypersalinica. The unclassified flagellate Palustrimonas, found in one Australian sample, proves to be a novel deep-branching alveolate. These results are consistent with a global distribution of halophilic protozoa groups (∼ morphospecies), but the Trimyema case suggests that is worth testing whether larger forms exhibit biogeographic phylogenetic substructure. The molecular detection/characterization of halophilic protozoa is still far from complete at the clade level, let alone the 'species level'. Copyright © 2015 Elsevier GmbH. All rights reserved.

452nd Brookhaven Lecture. Extreme Environments of Next-Generation Energy Systems and Materials: Can They Peacefully Co-Exist?

ScienceCinema

Simos, Nikolaos

2017-12-22

Nikolaos Simos of Brookhaven’s Energy Sciences and Technology Department and the National Synchrotron Light Source II Project presents, “Extreme Environments of Next-Generation Energy Systems and Materials: Can They Peacefully Co-Exist?”

40 CFR 355.14 - Do I have to aggregate extremely hazardous substances to determine the total quantity present?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 28 2011-07-01 2011-07-01 false Do I have to aggregate extremely hazardous substances to determine the total quantity present? 355.14 Section 355.14 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO...

A walk on the tundra: Host-parasite interactions in an extreme environment

USDA-ARS?s Scientific Manuscript database

The Arctic is a vast, sparsely populated region that has captured the imagination and interest of explorers and scientists for generations. It is an environment of extremes and has been considered by many an inhospitable landscape and the last frontier to be explored. Despite the ‘hardships’ impos...

Response of selected microoganisms to experimental planetary environments

NASA Technical Reports Server (NTRS)

Foster, T. L.

1975-01-01

A microbial population profile of mixed Cape Canaveral soil samples is presented. During this investigation a few organisms were isolated which exhibit the ability to grow at 3 C, 32 C, and 55 C. Growth curves are shown for three of these isolates, one of which grows extremely well at all three temperatures. Also included are studies dealing with growth of soil populations at zero and subzero temperatures. Results indicate growth at 0 C and -5 C, but not at 15 C or -65 C. The effect of storage temperature on dry soil is presented, and results show that psychrophilic populations decrease when soil is stored at room temperature, but do not decrease when soil is stored at -65 C. Results of an experiment with the simulated Martian environment are presented and indicate that nonsporeforming rods, sporeforming rods, and cocci can reproduce in the simulated environment when nutrients and moisture are supplied. The sporeforming rods are the predominant suvivors when dry soil is subjected to this environment.

PCR-based detection of micro-organisms in extreme environments during the EuroGeoMars MDRS campaign

NASA Astrophysics Data System (ADS)

Thiel, Cora S.; Ullrich, Oliver

Deoxyribonucleic acid (DNA) is found in all known living organisms and some viruses on earth. The main function of DNA molecules is the long-term storage of genetic information. They are passed on from generation to generation as the hereditary material. The polymerase chain reaction (PCR) is a revolutionary technique which allows amplifying a single or few copies of DNA molecules across several orders of magnitude, generating millions of copies of the original DNA fragment allowing detection of minimal traces of DNA. The compactness of the nowadays PCR instruments makes routine sample analysis possible with only a minimum of laboratory space. Our goal was to establish a routine for detection of DNA from micro-organisms based on the effective but also robust and simple PCR technique during the EuroGeoMars simula-tion campaign at The Mars Society's Mars Desert Research Station (MDRS) in February 2009. During the MDRS simulation we were able to show that it is possible to establish a minimal molecular biology lab in the habitat for an immediate on-site analysis by PCR after sample collection. Soil and water samples were taken from different locations and soil depths. The sample analysis was started immediately after returning to the habitat and was completed dur-ing the following days. DNA was isolated from micro-organisms and was used as a template for PCR analysis of the highly conserved ribosomal DNA to identify representatives of the different groups of micro-organisms (archaea, bacteria, eukaryotes). PCR products were visualized by agarose gel electrophoresis and documented by UV-transilluminator and digital camera. For the first time it was possible to demonstrate a direct on-site DNA analysis by PCR at MDRS, situated in an extreme environment that functions as a model for preparation and optimization of techniques to be used for future Mars exploration.

Bacterial diversity in fumarole environments of the Paricutín volcano, Michoacán (Mexico).

PubMed

Medrano-Santillana, Miguel; Souza-Brito, Elcia Margaret; Duran, Robert; Gutierrez-Corona, Felix; Reyna-López, Georgina Elena

2017-05-01

Active volcanoes are among the most extreme environments on Earth. The extreme temperatures, presence of toxic heavy metals and low nutrient bioavailability favor the development of extremophiles. We characterized the physical-chemical parameters of and bacterial communities (T-RFLP and 16S rRNA gene libraries) inhabiting fumarole niches of the Paricutín volcano located in Michoacán (Mexico). This volcano, which surged in 1943, is one of the youngest volcanoes on Earth and the microbial diversity in this area is yet to be characterized. The sampling stations were characterized in a pH range from 5.34 to 7.89 and showed different temperatures (soil, 27-87 °C; air, 13.6-56 °C) with high concentrations of metals such as iron and arsenic. The most abundant bacterial populations, confirmed by T-RFLP and 16S rRNA gene libraries, were related to members of Firmicutes and Proteobacteria phyla including sequences associated with thermophiles and sulfate reducing bacteria. Overall, the Paricutín volcano showed low bacterial diversity and its prokaryotic diversity was characterized by the impossibility of amplifying Archaea-related sequences.

Joint Probability Analysis of Extreme Precipitation and Storm Tide in a Coastal City under Changing Environment

PubMed Central

Xu, Kui; Ma, Chao; Lian, Jijian; Bin, Lingling

2014-01-01

Catastrophic flooding resulting from extreme meteorological events has occurred more frequently and drawn great attention in recent years in China. In coastal areas, extreme precipitation and storm tide are both inducing factors of flooding and therefore their joint probability would be critical to determine the flooding risk. The impact of storm tide or changing environment on flooding is ignored or underestimated in the design of drainage systems of today in coastal areas in China. This paper investigates the joint probability of extreme precipitation and storm tide and its change using copula-based models in Fuzhou City. The change point at the year of 1984 detected by Mann-Kendall and Pettitt’s tests divides the extreme precipitation series into two subsequences. For each subsequence the probability of the joint behavior of extreme precipitation and storm tide is estimated by the optimal copula. Results show that the joint probability has increased by more than 300% on average after 1984 (α = 0.05). The design joint return period (RP) of extreme precipitation and storm tide is estimated to propose a design standard for future flooding preparedness. For a combination of extreme precipitation and storm tide, the design joint RP has become smaller than before. It implies that flooding would happen more often after 1984, which corresponds with the observation. The study would facilitate understanding the change of flood risk and proposing the adaption measures for coastal areas under a changing environment. PMID:25310006

Joint probability analysis of extreme precipitation and storm tide in a coastal city under changing environment.

PubMed

Xu, Kui; Ma, Chao; Lian, Jijian; Bin, Lingling

2014-01-01

Catastrophic flooding resulting from extreme meteorological events has occurred more frequently and drawn great attention in recent years in China. In coastal areas, extreme precipitation and storm tide are both inducing factors of flooding and therefore their joint probability would be critical to determine the flooding risk. The impact of storm tide or changing environment on flooding is ignored or underestimated in the design of drainage systems of today in coastal areas in China. This paper investigates the joint probability of extreme precipitation and storm tide and its change using copula-based models in Fuzhou City. The change point at the year of 1984 detected by Mann-Kendall and Pettitt's tests divides the extreme precipitation series into two subsequences. For each subsequence the probability of the joint behavior of extreme precipitation and storm tide is estimated by the optimal copula. Results show that the joint probability has increased by more than 300% on average after 1984 (α = 0.05). The design joint return period (RP) of extreme precipitation and storm tide is estimated to propose a design standard for future flooding preparedness. For a combination of extreme precipitation and storm tide, the design joint RP has become smaller than before. It implies that flooding would happen more often after 1984, which corresponds with the observation. The study would facilitate understanding the change of flood risk and proposing the adaption measures for coastal areas under a changing environment.

Optimization of whole-transcriptome amplification from low cell density deep-sea microbial samples for metatranscriptomic analysis.

PubMed

Wu, Jieying; Gao, Weimin; Zhang, Weiwen; Meldrum, Deirdre R

2011-01-01

Limitation in sample quality and quantity is one of the big obstacles for applying metatranscriptomic technologies to explore gene expression and functionality of microbial communities in natural environments. In this study, several amplification methods were evaluated for whole-transcriptome amplification of deep-sea microbial samples, which are of low cell density and high impurity. The best amplification method was identified and incorporated into a complete protocol to isolate and amplify deep-sea microbial samples. In the protocol, total RNA was first isolated by a modified method combining Trizol (Invitrogen, CA) and RNeasy (QIAGEN, CA) method, amplified with a WT-Ovation™ Pico RNA Amplification System (NuGEN, CA), and then converted to double-strand DNA from single-strand cDNA with a WT-Ovation™ Exon Module (NuGEN, CA). The products from the whole-transcriptome amplification of deep-sea microbial samples were assessed first through random clone library sequencing. The BLAST search results showed that marine-based sequences are dominant in the libraries, consistent with the ecological source of the samples. The products were then used for next-generation Roche GS FLX Titanium sequencing to obtain metatranscriptome data. Preliminary analysis of the metatranscriptomic data showed good sequencing quality. Although the protocol was designed and demonstrated to be effective for deep-sea microbial samples, it should be applicable to similar samples from other extreme environments in exploring community structure and functionality of microbial communities. Copyright © 2010 Elsevier B.V. All rights reserved.

Slice sampling technique in Bayesian extreme of gold price modelling

NASA Astrophysics Data System (ADS)

Rostami, Mohammad; Adam, Mohd Bakri; Ibrahim, Noor Akma; Yahya, Mohamed Hisham

2013-09-01

In this paper, a simulation study of Bayesian extreme values by using Markov Chain Monte Carlo via slice sampling algorithm is implemented. We compared the accuracy of slice sampling with other methods for a Gumbel model. This study revealed that slice sampling algorithm offers more accurate and closer estimates with less RMSE than other methods . Finally we successfully employed this procedure to estimate the parameters of Malaysia extreme gold price from 2000 to 2011.

Effects of Radiation and Long-Term Thermal Cycling on EPC 1001 Gallium Nitride Transistors

NASA Technical Reports Server (NTRS)

Patterson, Richard L.; Scheick, Leif; Lauenstein, Jean-Marie; Casey, Megan; Hammoud, Ahmad

2012-01-01

Electronics designed for use in NASA space missions are required to work efficiently and reliably under harsh environment conditions. These include radiation, extreme temperatures, and thermal cycling, to name a few. Data obtained on long-term thermal cycling of new un-irradiated and irradiated samples of EPC1001 gallium nitride enhancement-mode transistors are presented. This work was done by a collaborative effort including GRC, GSFC, and support the NASA www.nasa.gov 1 JPL in of Electronic Parts and Packaging (NEPP) Program

Chest Ultrasonography in Modern Day Extreme Settings: From Military Setting and Natural Disasters to Space Flights and Extreme Sports

PubMed Central

Mucci, Viviana

2018-01-01

Chest ultrasonography (CU) is a noninvasive imaging technique able to provide an immediate diagnosis of the underlying aetiology of acute respiratory failure and traumatic chest injuries. Given the great technologies, it is now possible to perform accurate CU in remote and adverse environments including the combat field, extreme sport settings, and environmental disasters, as well as during space missions. Today, the usage of CU in the extreme emergency setting is more likely to occur, as this technique proved to be a fast diagnostic tool to assist resuscitation manoeuvres and interventional procedures in many cases. A scientific literature review is presented here. This was based on a systematic search of published literature, on the following online databases: PubMed and Scopus. The following words were used: “chest sonography,” “ thoracic ultrasound,” and “lung sonography,” in different combinations with “extreme sport,” “extreme environment,” “wilderness,” “catastrophe,” and “extreme conditions.” This manuscript reports the most relevant usages of CU in the extreme setting as well as technological improvements and current limitations. CU application in the extreme setting is further encouraged here. PMID:29736195

Genomics and Metagenomics of Extreme Acidophiles in Biomining Environments

NASA Astrophysics Data System (ADS)

Holmes, D. S.

2015-12-01

Over 160 draft or complete genomes of extreme acidophiles (pH < 3) have been published, many of which are from bioleaching and other biomining environments, or are closely related to such microorganisms. In addition, there are over 20 metagenomic studies of such environments. This provides a rich source of latent data that can be exploited for understanding the biology of biomining environments and for advancing biotechnological applications. Genomic and metagenomic data are already yielding valuable insights into cellular processes, including carbon and nitrogen management, heavy metal and acid resistance, iron and sulfur oxido-reduction, linking biogeochemical processes to organismal physiology. The data also allow the construction of useful models of the ecophysiology of biomining environments and provide insight into the gene and genome evolution of extreme acidophiles. Additionally, since most of these acidophiles are also chemoautolithotrophs that use minerals as energy sources or electron sinks, their genomes can be plundered for clues about the evolution of cellular metabolism and bioenergetic pathways during the Archaean abiotic/biotic transition on early Earth. Acknowledgements: Fondecyt 1130683.

Instrumentation for in situ sampling and analysis of compounds of interest to Astrobiology in the lower atmosphere and surface of Titan

NASA Technical Reports Server (NTRS)

Holland, Paul M.; Kojiro, Daniel R.; Stimac, Robert; Kaye, William; Takeuchi, Nori

2006-01-01

Instrumentation for exploration of the solar system will require new enabling technology for in situ sample acquisition and analysis of pre-biotic chemistry in extreme planetary environments, such as that encountered at the surface of Titan. The potential use of balloon aero-rovers for Titan places special emphasis on the importance of miniaturization, low power and low usage of consumables. To help meet this need, we are developing a miniature gas chromatograph coupled with a new Mini-Cell ion mobility spectrometer (GC-IMS), and one of us (PMH) has begun development work on a miniaturized cryogenic inlet system with sampling probes for Titan. This instrumentation, and its approach for meeting measurement needs for the analysis of prebiotic chemistry on Titan, will be discussed.

Robust, Thin Optical Films for Extreme Environments

NASA Technical Reports Server (NTRS)

2006-01-01

The environment of space presents scientists and engineers with the challenges of a harsh, unforgiving laboratory in which to conduct their scientific research. Solar astronomy and X-ray astronomy are two of the more challenging areas into which NASA scientists delve, as the optics for this high-tech work must be extremely sensitive and accurate, yet also be able to withstand the battering dished out by radiation, extreme temperature swings, and flying debris. Recent NASA work on this rugged equipment has led to the development of a strong, thin film for both space and laboratory use.

Toward Reliable and Energy Efficient Wireless Sensing for Space and Extreme Environments

NASA Technical Reports Server (NTRS)

Choi, Baek-Young; Boyd, Darren; Wilkerson, DeLisa

2017-01-01

Reliability is the critical challenge of wireless sensing in space systems operating in extreme environments. Energy efficiency is another concern for battery powered wireless sensors. Considering the physics of wireless communications, we propose an approach called Software-Defined Wireless Communications (SDC) that dynamically decide a reliable channel(s) avoiding unnecessary redundancy of channels, out of multiple distinct electromagnetic frequency bands such as radio and infrared frequencies.We validate the concept with Android and Raspberry Pi sensors and pseudo extreme experiments. SDC can be utilized in many areas beyond space applications.

Differentially expressed drought-responsive genes in Malus sieversii roots may contribute to this species' adaptation to arid environments

USDA-ARS?s Scientific Manuscript database

Understanding naturally evolved adaptation to arid climates may be a key factor in developing crops that can thrive during extreme climate fluctuations. Malus sieversii (Ledeb.) M. Roem. is a wild apple species that has adapted to harsh environments in Kazakhstan, including extreme cold and dry reg...

40 CFR 355.13 - How do I calculate the quantity of an extremely hazardous substance present in mixtures?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 28 2011-07-01 2011-07-01 false How do I calculate the quantity of an extremely hazardous substance present in mixtures? 355.13 Section 355.13 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS...

Flexible Electronics-Based Transformers for Extreme Environments

NASA Technical Reports Server (NTRS)

Quadrelli, Marco B.; Stoica, Adrian; Ingham, Michel; Thakur, Anubhav

2015-01-01

This paper provides a survey of the use of modular multifunctional systems, called Flexible Transformers, to facilitate the exploration of extreme and previously inaccessible environments. A novel dynamics and control model of a modular algorithm for assembly, folding, and unfolding of these innovative structural systems is also described, together with the control model and the simulation results.

Extreme Environment Capable, Modular and Scalable Power Processing Unit for Solar Electric Propulsion

NASA Technical Reports Server (NTRS)

Carr, Gregory A.; Iannello, Christopher J.; Chen, Yuan; Hunter, Don J.; DelCastillo, Linda; Bradley, Arthur T.; Stell, Christopher; Mojarradi, Mohammad M.

2013-01-01

This paper is to present a concept of a modular and scalable High Temperature Boost (HTB) Power Processing Unit (PPU) capable of operating at temperatures beyond the standard military temperature range. The various extreme environments technologies are also described as the fundamental technology path to this concept. The proposed HTB PPU is intended for power processing in the area of space solar electric propulsion, where reduction of in-space mass and volume are desired, and sometimes even critical, to achieve the goals of future space flight missions. The concept of the HTB PPU can also be applied to other extreme environment applications, such as geothermal and petroleum deep-well drilling, where higher temperature operation is required.

Extreme Environment Capable, Modular and Scalable Power Processing Unit for Solar Electric Propulsion

NASA Technical Reports Server (NTRS)

Carr, Gregory A.; Iannello, Christopher J.; Chen, Yuan; Hunter, Don J.; Del Castillo, Linda; Bradley, Arthur T.; Stell, Christopher; Mojarradi, Mohammad M.

2013-01-01

This paper is to present a concept of a modular and scalable High Temperature Boost (HTB) Power Processing Unit (PPU) capable of operating at temperatures beyond the standard military temperature range. The various extreme environments technologies are also described as the fundamental technology path to this concept. The proposed HTB PPU is intended for power processing in the area of space solar electric propulsion, where the reduction of in-space mass and volume are desired, and sometimes even critical, to achieve the goals of future space flight missions. The concept of the HTB PPU can also be applied to other extreme environment applications, such as geothermal and petroleum deep-well drilling, where higher temperature operation is required.

Development and Testing of Mechanism Technology for Space Exploration in Extreme Environments

NASA Technical Reports Server (NTRS)

Tyler, Tony R.; Levanas, Greg; Mojarradi, Mohammad M.; Abel, Phillip B.

2011-01-01

The NASA Jet Propulsion Lab (JPL), Glenn Research Center (GRC), Langley Research Center (LaRC), and Aeroflex, Inc. have partnered to develop and test actuator hardware that will survive the stringent environment of the moon, and which can also be leveraged for other challenging space exploration missions. Prototype actuators have been built and tested in a unique low temperature test bed with motor interface temperatures as low as 14 degrees Kelvin. Several years of work have resulted in specialized electro-mechanical hardware to survive extreme space exploration environments, a test program that verifies and finds limitations of the designs at extreme temperatures, and a growing knowledge base that can be leveraged by future space exploration missions.

Terrestrial environment (Climatic) criteria guidelines for use in aerospace vehicle development, 1977 revision

NASA Technical Reports Server (NTRS)

Kaufman, J. W. (Editor)

1977-01-01

Guidelines are provided on terrestrial environment data specifically applicable for NASA aerospace vehicles and associated equipment development. Information is included on the general distribution of natural environment extremes in the conterminous United States that may be needed to specify design criteria in the transportation of space vehicle subsystems and components. Atmospheric attenuation was investigated since certain earth orbital experiment missions are influenced by the earth's atmosphere. A summary of climatic extremes for worldwide operational needs is also included. The latest available information on probable climatic extremes is presented with information on atmospheric chemistry, seismic criteria, and on a mathematical model to predict atmospheric dispersion of aerospace engine exhaust cloud rise and growth. Cloud phenomena are also considered.

EFFECT OF ENVIRONMENT ON GALAXIES' MASS-SIZE DISTRIBUTION: UNVEILING THE TRANSITION FROM OUTSIDE-IN TO INSIDE-OUT EVOLUTION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cappellari, Michele

2013-11-20

The distribution of galaxies on the mass-size plane as a function of redshift or environment is a powerful test for galaxy formation models. Here we use integral-field stellar kinematics to interpret the variation of the mass-size distribution in two galaxy samples spanning extreme environmental densities. The samples are both identically and nearly mass-selected (stellar mass M {sub *} ≳ 6 × 10{sup 9} M {sub ☉}) and volume-limited. The first consists of nearby field galaxies from the ATLAS{sup 3D} parent sample. The second consists of galaxies in the Coma Cluster (Abell 1656), one of the densest environments for which good, resolvedmore » spectroscopy can be obtained. The mass-size distribution in the dense environment differs from the field one in two ways: (1) spiral galaxies are replaced by bulge-dominated disk-like fast-rotator early-type galaxies (ETGs), which follow the same mass-size relation and have the same mass distribution as in the field sample; (2) the slow-rotator ETGs are segregated in mass from the fast rotators, with their size increasing proportionally to their mass. A transition between the two processes appears around the stellar mass M {sub crit} ≈ 2 × 10{sup 11} M {sub ☉}. We interpret this as evidence for bulge growth (outside-in evolution) and bulge-related environmental quenching dominating at low masses, with little influence from merging. In contrast, significant dry mergers (inside-out evolution) and halo-related quenching drives the mass and size growth at the high-mass end. The existence of these two processes naturally explains the diverse size evolution of galaxies of different masses and the separability of mass and environmental quenching.« less

High diversity and suggested endemicity of culturable Actinobacteria in an extremely oligotrophic desert oasis

PubMed Central

Arocha-Garza, Hector Fernando; Canales-Del Castillo, Ricardo; Eguiarte, Luis E.; Souza, Valeria

2017-01-01

The phylum Actinobacteria constitutes one of the largest and anciently divergent phyla within the Bacteria domain. Actinobacterial diversity has been thoroughly researched in various environments due to its unique biotechnological potential. Such studies have focused mostly on soil communities, but more recently marine and extreme environments have also been explored, finding rare taxa and demonstrating dispersal limitation and biogeographic patterns for Streptomyces. To test the distribution of Actinobacteria populations on a small scale, we chose the extremely oligotrophic and biodiverse Cuatro Cienegas Basin (CCB), an endangered oasis in the Chihuahuan desert to assess the diversity and uniqueness of Actinobacteria in the Churince System with a culture-dependent approach over a period of three years, using nine selective media. The 16S rDNA of putative Actinobacteria were sequenced using both bacteria universal and phylum-specific primer pairs. Phylogenetic reconstructions were performed to analyze OTUs clustering and taxonomic identification of the isolates in an evolutionary context, using validated type species of Streptomyces from previously phylogenies as a reference. Rarefaction analysis for total Actinobacteria and for Streptomyces isolates were performed to estimate species’ richness in the intermediate lagoon (IL) in the oligotrophic Churince system. A total of 350 morphologically and nutritionally diverse isolates were successfully cultured and characterized as members of the Phylum Actinobacteria. A total of 105 from the total isolates were successfully subcultured, processed for DNA extraction and 16S-rDNA sequenced. All strains belong to the order Actinomycetales, encompassing 11 genera of Actinobacteria; the genus Streptomyces was found to be the most abundant taxa in all the media tested throughout the 3-year sampling period. Phylogenetic analysis of our isolates and another 667 reference strains of the family Streptomycetaceae shows that our isolation effort produced 38 unique OTUs in six new monophyletic clades. This high biodiversity and uniqueness of Actinobacteria in an extreme oligotrophic environment, which has previously been reported for its diversity and endemicity, is a suggestive sign of microbial biogeography of Actinobacteria and it also represents an invaluable source of biological material for future ecological and bioprospecting studies. PMID:28480140

Depression among Indian university students and its association with perceived university academic environment, living arrangements and personal issues.

PubMed

Deb, Sibnath; Banu, Parveen R; Thomas, Shinto; Vardhan, R Vishnu; Rao, P Tirupathi; Khawaja, Nigar

2016-10-01

The aim of the study is to ascertain the level of depression among university students across gender, academic stream, semesters, perception of family environment and relationship with parents, academic performance, and family income. In addition, the study examines the association between students' perceived university academic environment, living arrangements, personal issues, and depression. Seven hypotheses were formulated for verification. A total of 717 students were recruited following the multistage cluster sampling method, and data were collected by a specially designed structured questionnaire, academic achievement record and a standardized University Students Depression Inventory. Findings disclosed that 37.7%, 13.1%, and 2.4% of the students were suffering from moderate, severe, and extremely severe depression. A significant difference was found across semester, that is, semester II students reported a higher level of depression than semester III students. So far as academic stream is concerned, students from humanities and social science were found to be suffering from more depression compared to students from science and management streams. The study further disclosed that the students who reported positive views about the university academic environment and living arrangements had lower level of depression compared to their counterparts. Personal resilience's such as being able to sharing personal problems with others and doing regular exercise were found to be associated with positive mental health. The findings of the study emphasize the need for immediate mental health support services for about 15.6% of the students who were either suffering from severe or extremely severe depression at the University. Copyright © 2016 Elsevier B.V. All rights reserved.

Coupled heat transfer model and experiment study of semitransparent barrier materials in aerothermal environment

NASA Astrophysics Data System (ADS)

Wang, Da-Lin; Qi, Hong

Semi-transparent materials (such as IR optical windows) are widely used for heat protection or transfer, temperature and image measurement, and safety in energy , space, military, and information technology applications. They are used, for instance, ceramic coatings for thermal barriers of spacecrafts or gas turbine blades, and thermal image observation under extreme or some dangerous environments. In this paper, the coupled conduction and radiation heat transfer model is established to describe temperature distribution of semitransparent thermal barrier medium within the aerothermal environment. In order to investigate this numerical model, one semi-transparent sample with black coating was considered, and photothermal properties were measured. At last, Finite Volume Method (FVM) was used to solve the coupled model, and the temperature responses from the sample surfaces were obtained. In addition, experiment study was also taken into account. In the present experiment, aerodynamic heat flux was simulated by one electrical heater, and two experiment cases were designed in terms of the duration of aerodynamic heating. One case is that the heater irradiates one surface of the sample continually until the other surface temperature up to constant, and the other case is that the heater works only 130 s. The surface temperature responses of these two cases were recorded. Finally, FVM model of the coupling conduction-radiation heat transfer was validated based on the experiment study with relative error less than 5%.

Exploring EUV Spicules Using 304 Angstrom He II Data from SDO AIA

NASA Technical Reports Server (NTRS)

Snyder, Ian R.; Sterling, Alphonse C.; Falconer, David A.; Moore, Ron L.

2014-01-01

We present results from a statistical study of He II 304 Angstrom Extreme Ultraviolet (EUV) spicules at the limb of the Sun. We also measured properties of one macrospicule; macrospicules are longer than most spicules, and much broader in width than spicules. We use high-cadence (12 second) and high-resolution (0.6 arcseconds pixels) resolution data from the Atmospheric Imaging Array (AIA) instrument on the Solar Dynamic Observatory (SDO). All of the observed events occurred near the solar north pole, where quiet Sun or coronal hole environments ensued. We examined the maximum lengths, maximum rise velocities, and lifetimes of 33 Extreme Ultraviolet (EUV) spicules and the macrospicule. For the bulk of the Extreme Ultraviolet (EUV) spicules these quantities are, respectively, approximately 10,000-40,000 kilometers, 20-100 kilometers per second, and approximately 100- approximately 1000 seconds. For the macrospicule the corresponding quantities were respectively approximately 60,000 kilometers, approximately 130 kilometers per second, approximately 1800 seconds, which is typical of macrospicules measured by other workers. Therefore macrospicules are taller, longer-lived, and faster than most Extreme Ultraviolet (EUV) spicules. The rise profiles of both the spicules and the macrospicules match well a second-order ("parabolic" ) trajectory, although the acceleration was often weaker than that of solar gravity in the profiles fitted to the trajectories. Our macrospicule also had an obvious brightening at its base at birth, while such brightening was not apparent for the Extreme Ultraviolet (EUV) spicules. Most of the Extreme Ultraviolet (EUV) spicules remained visible during their descent back to the solar surface, although a small percentage of the spicules and the macrospicule faded out before falling back to the surface. Our sample of macrospicules is not yet large enough to determine whether their initiation mechanism is identical to that of Extreme Ultraviolet (EUV) spicules.

The ESA-NASA 'CHOICE' Study: Winterover at Concordia Station, Interior Antarctica, as an Analog for Spaceflight-Associated Immune Dysregu1ation

NASA Technical Reports Server (NTRS)

Crucian, Brian E,; Feuerecker, M.; Salam, A. P.; Rybka, A.; Stowe, R. P.; Morrels, M.; Mehta, S. K.; Quiriarte, H.; Quintens, Roel; Thieme, U.;

Non-extremophilic 'extremophiles' - Archaeal dominance in the subsurface and their implication for life

NASA Astrophysics Data System (ADS)

Reitschuler, Christoph; Lins, Philipp; Illmer, Paul

2014-05-01

Archaea - besides bacteria and eukaryota constituting the third big domain of life - were so far regarded as typical inhabitants of extreme environments, as indicated by the name (Archaeon, Greek: 'original', 'primal'). Previous research and cultivation successes were basically carried out in habitats characterized by extreme temperature, pH and salinity regimes. Such extreme conditions, as expected at the beginning of the Earth's evolution, are occasionally also prevalent on extraterrestrial planets and moons and make the Archaeal domain a key group to be studied concerning life's evolution and the most likely pioneer organisms to colonize environments that are regarded as hostile. However, in recent years it became obvious that Archaea, in particular non-extremophilic species, can be found almost ubiquitously in marine, freshwater, terrestrial and also subsurface habitats and occasionally outnumber other microbial domains and hold key positions in globally relevant energy and nutrient cycles. Besides extreme environments - the big question remains how to define a parameter as extreme - subsurface and cave environments present a window to the past, where adaptions to early life's conditions can be studied and how microbiomes may be structured in a habitat that represents a refugium on extraterrestrial celestial bodies, were surface conditions might be at first sight too extreme for life. The lower part of the alpine Hundsalm cave in Tyrol (Austria) offered a unique opportunity to study an almost pristine cave habitat, which is separated from the touristic part of the ice cave. The main focus of our research was laid on the microbial communities that were supposed to be in connection with secondary carbonate precipitations ('moonmilk'). For the ascertainment of these so far poorly evaluated structures a multiple approach assessment was chosen to generate a virtually complete picture of these subsurface microbiomes. Thereby, a combination of different cultivation strategies was applied as well as physiological analyses, comprising HPLC and GC analyses, and molecular approaches, covering end-point and quantitative PCRs, DGGE, cloning, and sequencing analyses. Outstanding in the course of this research was that assumed non-extremophilic Archaea clearly outnumbered bacteria within the different moonmilk deposits, while fungi were only of minor importance. Moreover, the Archaeal species formed a constant element within the investigated samples, while bacteria and fungi showed a much more diverse and inhomogeneous community pattern. This indicates that the Archaea might constitute the central element within the microbial communities, holding key positions in nutrient and energy-cycles. Furthermore, it was possible to cultivate the Archaeal community over a certain time period, demonstrating that so far uncultured or as 'not cultivable' regarded organisms are accessible with certain cultivation strategies. On the other hand the vast majority of bacterial and fungal representatives are in close relation to species, which are valuable concerning biotechnological or medical applications. Summing up, further research on cave microbiomes, especially Archaea, might be reasonable with regards to life's evolution, global nutrient cycles, requirements for possible refuges of extraterrestrial life forms and also concerning new technological applications.

Genomic Basis of Adaptive Evolution: The Survival of Amur Ide (Leuciscus waleckii) in an Extremely Alkaline Environment

PubMed Central

Xu, Jian; Li, Jiong-Tang; Jiang, Yanliang; Peng, Wenzhu; Yao, Zongli; Chen, Baohua; Jiang, Likun; Feng, Jingyan; Ji, Peifeng; Liu, Guiming; Liu, Zhanjiang; Tai, Ruyu; Dong, Chuanju; Sun, Xiaoqing; Zhao, Zi-Xia; Zhang, Yan; Wang, Jian; Li, Shangqi; Zhao, Yunfeng; Yang, Jiuhui; Sun, Xiaowen; Xu, Peng

2017-01-01

The Amur ide (Leuciscus waleckii) is a cyprinid fish that is widely distributed in Northeast Asia. The Lake Dali Nur population inhabits one of the most extreme aquatic environments on Earth, with an alkalinity up to 50 mmol/L (pH 9.6), thus providing an exceptional model with which to characterize the mechanisms of genomic evolution underlying adaptation to extreme environments. Here, we developed the reference genome assembly for L. waleckii from Lake Dali Nur. Intriguingly, we identified unusual expanded long terminal repeats (LTRs) with higher nucleotide substitution rates than in many other teleosts, suggesting their more recent insertion into the L. waleckii genome. We also identified expansions in genes encoding egg coat proteins and natriuretic peptide receptors, possibly underlying the adaptation to extreme environmental stress. We further sequenced the genomes of 10 additional individuals from freshwater and 18 from Lake Dali Nur populations, and we detected a total of 7.6 million SNPs from both populations. In a genome scan and comparison of these two populations, we identified a set of genomic regions under selective sweeps that harbor genes involved in ion homoeostasis, acid-base regulation, unfolded protein response, reactive oxygen species elimination, and urea excretion. Our findings provide comprehensive insight into the genomic mechanisms of teleost fish that underlie their adaptation to extreme alkaline environments. PMID:28007977

Comet sample acquisition for ROSETTA lander mission

NASA Astrophysics Data System (ADS)

Marchesi, M.; Campaci, R.; Magnani, P.; Mugnuolo, R.; Nista, A.; Olivier, A.; Re, E.

2001-09-01

ROSETTA/Lander is being developed with a combined effort of European countries, coordinated by German institutes. The commitment for such a challenging probe will provide a unique opportunity for in-situ analysis of a comet nucleus. The payload for coring, sampling and investigations of comet materials is called SD2 (Sampling Drilling and Distribution). The paper presents the drill/sampler tool and the sample transfer trough modeling, design and testing phases. Expected drilling parameters are then compared with experimental data; limited torque consumption and axial thrust on the tool constraint the operation and determine the success of tests. Qualification campaign involved the structural part and related vibration test, the auger/bit parts and drilling test, and the coring mechanism with related sampling test. Mechanical check of specimen volume is also reported, with emphasis on the measurement procedure and on the mechanical unit. The drill tool and all parts of the transfer chain were tested in the hypothetical comet environment, charcterized by frozen material at extreme low temperature and high vacuum (-160°C, 10-3 Pa).

Early Detection of Lightning Caused Wildfires and Prediction of Wildfire Behavior through Energy Distribution, Atmospherics, Geophysics, the Sun's Azimuth, and Topology

NASA Astrophysics Data System (ADS)

Giesige, C.; Nava, E.

2016-12-01

In the midst of a changing climate we have seen extremes in weather events: lightning, wildfires, hurricanes, tornadoes, and earthquakes. All of these ride on an imbalance of magnetic and electrical distribution about the earth including what goes on from the atmospheric and geophysic levels. There is relevance to the important role the sun plays in developing and feeding of the extreme weather events along with the sun's role helping to create a separation of charges on earth furthering climactic extremes. Focusing attention in North America and on how the sun, atmospheric and geophysic winds come together producing lightning events, there are connections between energy distribution in the environment, lightning caused wildfires, and extreme wildfire behavior. Lightning caused wildfires and extreme fire behavior have become enhanced with the changing climate conditions. Even with strong developments in wildfire science, there remains a lack in full understanding of connections that create a lightning caused wildfire event and lack of monitoring advancements in predicting extreme fire behavior. Several connections have been made in our research allowing us to connect multiple facets of the environment in regards to electric and magnetic influences on wildfires. Among them include: irradiance, winds, pressure systems, humidity, and topology. The connections can be made to develop better detection systems of wildfires, establish with more accuracy areas of highest risk for wildfire and extreme wildfire behavior, and prediction of wildfire behavior. A platform found within the environment can also lead to further understanding and monitoring of other extreme weather events in the future.

Fabrication of diamond based sensors for use in extreme environments

DOE PAGES

Samudrala, Gopi K.; Moore, Samuel L.; Vohra, Yogesh K.

2015-04-23

Electrical and magnetic sensors can be lithographically fabricated on top of diamond substrates and encapsulated in a protective layer of chemical vapor deposited single crystalline diamond. This process when carried out on single crystal diamond anvils employed in high pressure research is termed as designer diamond anvil fabrication. These designer diamond anvils allow researchers to study electrical and magnetic properties of materials under extreme conditions without any possibility of damaging the sensing elements. We describe a novel method for the fabrication of designer diamond anvils with the use of maskless lithography and chemical vapor deposition in this paper. This methodmore » can be utilized to produce diamond based sensors which can function in extreme environments of high pressures, high and low temperatures, corrosive and high radiation conditions. Here, we demonstrate applicability of these diamonds under extreme environments by performing electrical resistance measurements during superconducting transition in rare earth doped iron-based compounds under high pressures to 12 GPa and low temperatures to 10 K.« less

Fabrication of diamond based sensors for use in extreme environments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Samudrala, Gopi K.; Moore, Samuel L.; Vohra, Yogesh K.

Electrical and magnetic sensors can be lithographically fabricated on top of diamond substrates and encapsulated in a protective layer of chemical vapor deposited single crystalline diamond. This process when carried out on single crystal diamond anvils employed in high pressure research is termed as designer diamond anvil fabrication. These designer diamond anvils allow researchers to study electrical and magnetic properties of materials under extreme conditions without any possibility of damaging the sensing elements. We describe a novel method for the fabrication of designer diamond anvils with the use of maskless lithography and chemical vapor deposition in this paper. This methodmore » can be utilized to produce diamond based sensors which can function in extreme environments of high pressures, high and low temperatures, corrosive and high radiation conditions. Here, we demonstrate applicability of these diamonds under extreme environments by performing electrical resistance measurements during superconducting transition in rare earth doped iron-based compounds under high pressures to 12 GPa and low temperatures to 10 K.« less

Research on Nitride Thin Films, Advanced Plasma Diagnostics, and Charged-Particle Processes

DTIC Science & Technology

2006-07-01

Additionally, these components are being placed closer to the point of use--requiring that they operate in extreme temperature environments ...reasons for component failure. To operate in extreme temperature environments , electronic and electrical components must withstand higher ambient...hybrid and plug-in hybrid-powered automobiles, heart defibrillators , and industrial equipment will benefit from a new generation of capacitors. High

40 CFR Appendix B to Part 355 - The List of Extremely Hazardous Substances and Their Threshold Planning Quantities

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 29 2012-07-01 2012-07-01 false The List of Extremely Hazardous Substances and Their Threshold Planning Quantities B Appendix B to Part 355 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS EMERGENCY PLANNING AND NOTIFICATION Pt....

40 CFR Appendix A to Part 355 - The List of Extremely Hazardous Substances and Their Threshold Planning Quantities

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 28 2014-07-01 2014-07-01 false The List of Extremely Hazardous Substances and Their Threshold Planning Quantities A Appendix A to Part 355 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS EMERGENCY PLANNING AND NOTIFICATION Pt....

40 CFR Appendix A to Part 355 - The List of Extremely Hazardous Substances and Their Threshold Planning Quantities

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 29 2012-07-01 2012-07-01 false The List of Extremely Hazardous Substances and Their Threshold Planning Quantities A Appendix A to Part 355 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS EMERGENCY PLANNING AND NOTIFICATION Pt....

40 CFR Appendix B to Part 355 - The List of Extremely Hazardous Substances and Their Threshold Planning Quantities

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 28 2014-07-01 2014-07-01 false The List of Extremely Hazardous Substances and Their Threshold Planning Quantities B Appendix B to Part 355 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS EMERGENCY PLANNING AND NOTIFICATION Pt....

Method for reworkable packaging of high speed, low electrical parasitic power electronics modules through gate drive integration

DOEpatents

Passmore, Brandon; Cole, Zach; Whitaker, Bret; Barkley, Adam; McNutt, Ty; Lostetter, Alexander

2016-08-02

A multichip power module directly connecting the busboard to a printed-circuit board that is attached to the power substrate enabling extremely low loop inductance for extreme environments such as high temperature operation. Wire bond interconnections are taught from the power die directly to the busboard further enabling enable low parasitic interconnections. Integration of on-board high frequency bus capacitors provide extremely low loop inductance. An extreme environment gate driver board allows close physical proximity of gate driver and power stage to reduce overall volume and reduce impedance in the control circuit. Parallel spring-loaded pin gate driver PCB connections allows a reliable and reworkable power module to gate driver interconnections.

New controlled environment vitrification system for preparing wet samples for cryo-SEM.

PubMed

Ge, H; Suszynski, W J; Davis, H T; Scriven, L E

2008-01-01

A new controlled environment vitrification system (CEVS) has been designed and constructed to facilitate examination by cryogenic scanning electron microscopy (Cryo-SEM) of initial suspension state and of microstructure development in latex, latex-composite and other coatings while they still contain solvent. The new system has a main chamber with provisions for coating as well as drying, and for well-controlled plunging into cryogen. An added subsidiary chamber holds samples for drying or annealing over minutes to days before they are returned to the main chamber and plunged from it. In the main chamber, samples are blade-coated on 5 x 7 mm pieces of silicon wafer and held at selected temperature and humidity for successively longer times, either there or after transfer along a rail into the subsidiary chamber. They are then placed in the sample holder mounted on the plunge rod, so as to permit adjustment of the sample's attitude when it plunges, at controlled speed, into liquid ethane at its freezing point, to a chosen depth, in order to solidify the sample without significant shear or freezing artifacts. The entries of plunging samples and related sample holders into liquid ethane were recorded with a high-speed, high-resolution Photron digital camera. The data were interpreted with a new hypothesis about the width of the band of extremely rapid cooling by deeply subcooled nucleate boiling below the line of entry. Complementary cryo-SEM images revealed that the freezing rate and surface shearing of a sample need to be balanced by adjusting the plunging attitude.

The quality of visual information about the lower extremities influences visuomotor coordination during virtual obstacle negotiation.

PubMed

Kim, Aram; Kretch, Kari S; Zhou, Zixuan; Finley, James M

2018-05-09

Successful negotiation of obstacles during walking relies on the integration of visual information about the environment with ongoing locomotor commands. When information about the body and environment are removed through occlusion of the lower visual field, individuals increase downward head pitch angle, reduce foot placement precision, and increase safety margins during crossing. However, whether these effects are mediated by loss of visual information about the lower extremities, the obstacle, or both remains to be seen. Here, we used a fully immersive, virtual obstacle negotiation task to investigate how visual information about the lower extremities is integrated with information about the environment to facilitate skillful obstacle negotiation. Participants stepped over virtual obstacles while walking on a treadmill with one of three types of visual feedback about the lower extremities: no feedback, end-point feedback, or a link-segment model. We found that absence of visual information about the lower extremities led to an increase in the variability of leading foot placement after crossing. The presence of a visual representation of the lower extremities promoted greater downward head pitch angle during the approach to and subsequent crossing of an obstacle. In addition, having greater downward head pitch was associated with closer placement of the trailing foot to the obstacle, further placement of the leading foot after the obstacle, and higher trailing foot clearance. These results demonstrate that the fidelity of visual information about the lower extremities influences both feed-forward and feedback aspects of visuomotor coordination during obstacle negotiation.

Bacterial Diversity in Microbial Mats and Sediments from the Atacama Desert.

PubMed

Rasuk, Maria Cecilia; Fernández, Ana Beatriz; Kurth, Daniel; Contreras, Manuel; Novoa, Fernando; Poiré, Daniel; Farías, María Eugenia

2016-01-01

The Atacama Desert has extreme environmental conditions that allow the development of unique microbial communities. The present paper reports the bacterial diversity of microbial mats and sediments and its mineralogical components. Some physicochemical conditions of the water surrounding these ecosystems have also been studied trying to determine their influence on the diversity of these communities. In that way, mats and sediments distributed among different hypersaline lakes located in salt flats of the Atacama Desert were subjected to massive parallel sequencing of the V4 region of the 16S rRNA genes of Bacteria. A higher diversity in sediment than in mat samples have been found. Lakes that harbor microbial mats have higher salinity than lakes where mats are absent. Proteobacteria and/or Bacteroidetes are the major phyla represented in all samples. An interesting item is the finding of a low proportion or absence of Cyanobacteria sequences in the ecosystems studied, suggesting the possibility that other groups may be playing an essential role as primary producers in these extreme environments. Additionally, the large proportion of 16S rRNA gene sequences that could not be classified at the level of phylum indicates potential new phyla present in these ecosystems.

When high waters recede and the floodplain reemerges: Evaluating the lingering effects of extreme flooding on stream nitrogen cycling.

NASA Astrophysics Data System (ADS)

Neville, J.; Emanuel, R. E.

2017-12-01

In 2016 Hurricane Matthew brought immense flooding and devastation to the Lumbee (aka Lumber) River basin. Some impacts are obvious, such as deserted homes and businesses, but other impacts, including long-term environmental, are uncertain. Extreme flooding throughout the basin established temporary hydrologic connectivity between aquatic environments and upland sources of nutrients and other pollutants. Though 27% of the basin is covered by wetlands, hurricane-induced flooding was so intense that wetlands may have had no opportunity to mitigate delivery of nutrients into surface waters. As a result, how Hurricane Matthew impacted nitrate retention and uptake in the Lumbee River remains uncertain. The unknown magnitude of nitrate transported into the Lumbee River from surrounding sources may have lingering impacts on nitrogen cycling in this stream. With these potential impacts in mind, we conducted a Lagrangian water quality sampling campaign to assess the ability of the Lumbee River to retain and process nitrogen following Hurricane Matthew. We collected samples before and after flooding and compare first order nitrogen uptake kinetics of both periods. The analysis and comparisons allow us to evaluate the long-term impacts of Hurricane Matthew on nitrogen cycling after floodwaters recede.

Lightweight Modular Instrumentation for Planetary Applications

NASA Technical Reports Server (NTRS)

Joshi, P. B.

1993-01-01

An instrumentation, called Space Active Modular Materials ExperimentS (SAMMES), is developed for monitoring the spacecraft environment and for accurately measuring the degradation of space materials in low earth orbit (LEO). The SAMMES architecture concept can be extended to instrumentation for planetary exploration, both on spacecraft and in situ. The operating environment for planetary application will be substantially different, with temperature extremes and harsh solar wind and cosmic ray flux on lunar surfaces and temperature extremes and high winds on venusian and Martian surfaces. Moreover, instruments for surface deployment, which will be packaged in a small lander/rover (as in MESUR, for example), must be extremely compact with ultralow power and weight. With these requirements in mind, the SAMMES concept was extended to a sensor/instrumentation scheme for the lunar and Martian surface environment.

Galaxy Zoo: the interplay of quenching mechanisms in the group environment★

NASA Astrophysics Data System (ADS)

Smethurst, R. J.; Lintott, C. J.; Bamford, S. P.; Hart, R. E.; Kruk, S. J.; Masters, K. L.; Nichol, R. C.; Simmons, B. D.

2017-08-01

Does the environment of a galaxy directly influence the quenching history of a galaxy? Here, we investigate the detailed morphological structures and star formation histories of a sample of SDSS group galaxies with both classifications from Galaxy Zoo 2 and near ultra-violet (NUV) detections in GALEX. We use the optical and NUV colours to infer the quenching time and rate describing a simple exponentially declining star formation history for each galaxy, along with a control sample of field galaxies. We find that the time since quenching and the rate of quenching do not correlate with the relative velocity of a satellite but are correlated with the group potential. This quenching occurs within an average quenching time-scale of ˜ 2.5 Gyr from star forming to complete quiescence, during an average infall time (from ˜10R200 to 0.01R200) of ˜ 2.6 Gyr. Our results suggest that the environment does play a direct role in galaxy quenching through quenching mechanisms that are correlated with the group potential, such as harassment, interactions or starvation. Environmental quenching mechanisms that are correlated with satellite velocity, such as ram-pressure stripping, are not the main cause of quenching in the group environment. We find that no single mechanism dominates over another, except in the most extreme environments or masses. Instead, an interplay of mergers, mass and morphological quenching and environment-driven quenching mechanisms dependent on the group potential drive galaxy evolution in groups.

Tardigrades living in extreme environments have naturally selected prerequisites useful to space conquer

NASA Astrophysics Data System (ADS)

Guidetti, Roberto; Tiziana, Altiero; Cesari, Michele; Rizzo, Angela Maria; Bertolani, Roberto; Galletta, Giuseppe; Dalessandro, Maurizio; Rebecchi, Lorena

Extreme habitats are highly selective and can host only living organisms possessing specific adaptations to stressors. Among extreme habitats, space environment has particular charac-teristics of radiations, vacuum, microgravity and temperature, which induce rapid changes in living systems. Consequently, the response of multicellular complex organisms, able to colo-nize extreme environments, to space stresses can give very useful information on the ability to withstand a single stress or stress combinations. This knowledge on changes in living systems in space, with their similarity to the ageing processes, offers the opportunity to improve human life both on Earth and in space. Even though experimentation in space has often been carried out using unicellular organisms, multicellular organisms are very relevant in order to develop the appropriate countermeasures to avoid the risks imposed by environmental space in humans. The little attention received by multicellular organisms is probably due, other than to difficul-ties in the manipulation of biological materials in space, to the presence of only few organisms with the potential to tolerate environmental space stresses. Among them, tardigrades are small invertebrates representing an attractive animal model to study adaptive strategies for surviving extreme environments, including space environment. Tardigrades are little known microscopic aquatic animals (250-800 m in body length) distributed in different environments (from the deep sea to high mountains and deserts all over the world), and frequently inhabiting very unstable and unpredictable habitats (e.g. interstices of mosses, lichens, leaf litter, freshwater ponds, cryoconite holes). Their ability to live in the extreme environments is related to a wide variety of their life histories and adaptive strategies. A widespread and crucial strategy is cryptobiosis, a form of quiescence. It includes strategies such as anhydrobiosis and cryobiosis, characterized by a complete or almost complete metabolic standstill. The ability of tardigrades to colonize terrestrial habitats is linked to their well known ability to enter anhydrobiosis when their habi-tat desiccates. Tardigrades survive dehydration by entering a highly stable state of suspended animation due to complete desiccation (¿ 95Results on tardigrades open a window on the fu-ture perspective in astrobiology and in their applications. The discovery and identification of metabolites naturally synthesized by tardigrades to perform a remarkable protection against the damages to cellular components and DNA due to desiccation, radiation, microgravity and oxidation stresses, will be used to define the countermeasures to protect sensitive organisms, including humans, not naturally able to withstand extreme stresses under space conditions, for the future long-term explorations of our solar system, including Mars.

Effects of Wintering Environment and Parasite–Pathogen Interactions on Honey Bee Colony Loss in North Temperate Regions

PubMed Central

Currie, Robert W.

2016-01-01

Extreme winter losses of honey bee colonies are a major threat to beekeeping but the combinations of factors underlying colony loss remain debatable. We monitored colonies in two environments (colonies wintered indoors or outdoors) and characterized the effects of two parasitic mites, seven viruses, and Nosema on honey bee colony mortality and population loss over winter. Samples were collected from two locations within hives in fall, mid-winter and spring of 2009/2010. Although fall parasite and pathogen loads were similar in outdoor and indoor-wintered colonies, the outdoor-wintered colonies had greater relative reductions in bee population score over winter. Seasonal patterns in deformed wing virus (DWV), black queen cell virus (BQCV), and Nosema level also differed with the wintering environment. DWV and Nosema levels decreased over winter for indoor-wintered colonies but BQCV did not. Both BQCV and Nosema concentration increased over winter in outdoor-wintered colonies. The mean abundance of Varroa decreased and concentration of Sacbrood virus (SBV), Kashmir bee virus (KBV), and Chronic bee paralysis virus (CBPV) increased over winter but seasonal patterns were not affected by wintering method. For most viruses, either entrance or brood area samples were reasonable predictors of colony virus load but there were significant season*sample location interactions for Nosema and BQCV, indicating that care must be taken when selecting samples from a single location. For Nosema spp., the fall entrance samples were better predictors of future infestation levels than were fall brood area samples. For indoor-wintered colonies, Israeli acute paralysis virus IAPV concentration was negatively correlated with spring population size. For outdoor-wintered hives, spring Varroa abundance and DWV concentration were positively correlated with bee loss and negatively correlated with spring population size. Multivariate analyses for fall collected samples indicated higher DWV was associated with colony death as did high SBV for spring-collected samples. PMID:27448049

Using extreme phenotype sampling to identify the rare causal variants of quantitative traits in association studies.

PubMed

Li, Dalin; Lewinger, Juan Pablo; Gauderman, William J; Murcray, Cassandra Elizabeth; Conti, David

2011-12-01

Variants identified in recent genome-wide association studies based on the common-disease common-variant hypothesis are far from fully explaining the hereditability of complex traits. Rare variants may, in part, explain some of the missing hereditability. Here, we explored the advantage of the extreme phenotype sampling in rare-variant analysis and refined this design framework for future large-scale association studies on quantitative traits. We first proposed a power calculation approach for a likelihood-based analysis method. We then used this approach to demonstrate the potential advantages of extreme phenotype sampling for rare variants. Next, we discussed how this design can influence future sequencing-based association studies from a cost-efficiency (with the phenotyping cost included) perspective. Moreover, we discussed the potential of a two-stage design with the extreme sample as the first stage and the remaining nonextreme subjects as the second stage. We demonstrated that this two-stage design is a cost-efficient alternative to the one-stage cross-sectional design or traditional two-stage design. We then discussed the analysis strategies for this extreme two-stage design and proposed a corresponding design optimization procedure. To address many practical concerns, for example measurement error or phenotypic heterogeneity at the very extremes, we examined an approach in which individuals with very extreme phenotypes are discarded. We demonstrated that even with a substantial proportion of these extreme individuals discarded, an extreme-based sampling can still be more efficient. Finally, we expanded the current analysis and design framework to accommodate the CMC approach where multiple rare-variants in the same gene region are analyzed jointly. © 2011 Wiley Periodicals, Inc.

Using Extreme Phenotype Sampling to Identify the Rare Causal Variants of Quantitative Traits in Association Studies

PubMed Central

Li, Dalin; Lewinger, Juan Pablo; Gauderman, William J.; Murcray, Cassandra Elizabeth; Conti, David

2014-01-01

Variants identified in recent genome-wide association studies based on the common-disease common-variant hypothesis are far from fully explaining the hereditability of complex traits. Rare variants may, in part, explain some of the missing hereditability. Here, we explored the advantage of the extreme phenotype sampling in rare-variant analysis and refined this design framework for future large-scale association studies on quantitative traits. We first proposed a power calculation approach for a likelihood-based analysis method. We then used this approach to demonstrate the potential advantages of extreme phenotype sampling for rare variants. Next, we discussed how this design can influence future sequencing-based association studies from a cost-efficiency (with the phenotyping cost included) perspective. Moreover, we discussed the potential of a two-stage design with the extreme sample as the first stage and the remaining nonextreme subjects as the second stage. We demonstrated that this two-stage design is a cost-efficient alternative to the one-stage cross-sectional design or traditional two-stage design. We then discussed the analysis strategies for this extreme two-stage design and proposed a corresponding design optimization procedure. To address many practical concerns, for example measurement error or phenotypic heterogeneity at the very extremes, we examined an approach in which individuals with very extreme phenotypes are discarded. We demonstrated that even with a substantial proportion of these extreme individuals discarded, an extreme-based sampling can still be more efficient. Finally, we expanded the current analysis and design framework to accommodate the CMC approach where multiple rare-variants in the same gene region are analyzed jointly. PMID:21922541

Individual Differences in Adaptability to Isolated, Confined, and Extreme Environments.

PubMed

Bartone, Paul T; Krueger, Gerald P; Bartone, Jocelyn V

2018-06-01

Future deep space missions will expose astronauts to more intense stressors than previously encountered. Isolation will be greater and more prolonged, living and work areas more confined, and communications and resupply channels to Earth longer and less reliable. Astronauts will need to function more autonomously, with less guidance and support from Earth. Thus, it is important to select and train astronauts who can adapt and function effectively under extreme and variable conditions. In order to identify factors linked to individual adaptability, we conducted a systematic review of the literature on cognitive and behavioral adaptation to isolated, confined, and extreme (ICE) environments. We searched PubMed, Embase, Web of Science, and PsychINFO databases for studies addressing individual adaptability to ICE environments. Studies were rated for quality and fidelity to long-duration space missions and key results extracted. There were 73 studies that met all inclusion criteria. Adaptability attributes for ICE environments include intelligence, emotional stability, self-control, openness, achievement facets of conscientiousness, optimism, mastery, introversion, hardiness, task-oriented coping, past experience, low need for social support, and adequate sleep. This review identifies individual factors linked to adaptability under ICE conditions. Further studies are needed to verify causal directions and determine the relative importance of these factors.Bartone PT, Krueger GP, Bartone JV. Individual differences in adaptability to isolated, confined, and extreme environments. Aerosp Med Hum Perform. 2018; 89(6):536-546.

Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from Antarctica

NASA Astrophysics Data System (ADS)

Arenas, Felipe A.; Pugin, Benoit; Henríquez, Nicole A.; Arenas-Salinas, Mauricio A.; Díaz-Vásquez, Waldo A.; Pozo, María F.; Muñoz, Claudia M.; Chasteen, Thomas G.; Pérez-Donoso, José M.; Vásquez, Claudio C.

2014-03-01

The tellurium oxyanion, tellurite, is extremely noxious to most living organisms. Its toxicity has been mainly related to the generation of reactive oxygen species (ROS) as well as to an unbalancing of the thiol:redox buffering system. Nevertheless, a few bacteria are capable of thriving at high tellurite concentrations. One mechanism of resistance is the enzymatic and non-enzymatic reduction of tellurite to the less toxic elemental tellurium. This reduction generates nano- to micrometric tellurium crystals that display different shapes and sizes. To date, a very limited number of highly tellurite-resistant and tellurite-reducing bacterial species are available from international culture collections. In this work, we decided to look for tellurite-reducing bacteria from an extreme environment, Antarctica. This environment exhibits a combination of several extreme factors such as high UV-radiation and desiccation and freezing conditions that impact directly on the local biodiversity. Since, as does, all these factors induce ROS formation, we hypothesized that Antarctic bacteria could also exhibit tellurite-resistance. In this context, we isolated 123 tellurite-resistant bacteria, and characterized six new tellurite-resistant and tellurite-reducing bacterial strains from samples collected in Antarctica. These strains were identified according to their 16S rRNA gene sequence as Staphylococcus hameolyticus, Staphylococcus sciuri, Acinetobacter haemolyticus, Pseudomonas lini, and two strains of Psychrobacter immobilis. The isolates display tellurite-resistance about 35- to 500-fold higher than Escherichia coli (Te-sensitive organism), and a high level of tellurite reduction which might be interesting for an application in the field of bioremediation or nanoparticle biosynthesis.

Transitory microbial habitat in the hyperarid Atacama Desert

PubMed Central

Schulze-Makuch, Dirk; Wagner, Dirk; Mangelsdorf, Kai; Devine, Kevin G.; de Vera, Jean-Pierre; Parro, Victor; Kaupenjohann, Martin; Galy, Albert; Schneider, Beate; Airo, Alessandro; Frösler, Jan; Davila, Alfonso F.; Arens, Felix L.; Cáceres, Luis; Cornejo, Francisco Solís; Carrizo, Daniel; Dartnell, Lewis; DiRuggiero, Jocelyne; Ganzert, Lars; Gessner, Mark O.; Grathwohl, Peter; Guan, Lisa; Heinz, Jacob; Hess, Matthias; Keppler, Frank; Maus, Deborah; McKay, Christopher P.; Meckenstock, Rainer U.; Montgomery, Wren; Oberlin, Elizabeth A.; Probst, Alexander J.; Sáenz, Johan S.; Sattler, Tobias; Schirmack, Janosch; Sephton, Mark A.; Schloter, Michael; Uhl, Jenny; Valenzuela, Bernardita; Vestergaard, Gisle; Wörmer, Lars; Zamorano, Pedro

2018-01-01

Traces of life are nearly ubiquitous on Earth. However, a central unresolved question is whether these traces always indicate an active microbial community or whether, in extreme environments, such as hyperarid deserts, they instead reflect just dormant or dead cells. Although microbial biomass and diversity decrease with increasing aridity in the Atacama Desert, we provide multiple lines of evidence for the presence of an at times metabolically active, microbial community in one of the driest places on Earth. We base this observation on four major lines of evidence: (i) a physico-chemical characterization of the soil habitability after an exceptional rain event, (ii) identified biomolecules indicative of potentially active cells [e.g., presence of ATP, phospholipid fatty acids (PLFAs), metabolites, and enzymatic activity], (iii) measurements of in situ replication rates of genomes of uncultivated bacteria reconstructed from selected samples, and (iv) microbial community patterns specific to soil parameters and depths. We infer that the microbial populations have undergone selection and adaptation in response to their specific soil microenvironment and in particular to the degree of aridity. Collectively, our results highlight that even the hyperarid Atacama Desert can provide a habitable environment for microorganisms that allows them to become metabolically active following an episodic increase in moisture and that once it decreases, so does the activity of the microbiota. These results have implications for the prospect of life on other planets such as Mars, which has transitioned from an earlier wetter environment to today’s extreme hyperaridity. PMID:29483268

Bacterial biodiversity from anthropogenic extreme environments: a hyper-alkaline and hyper-saline industrial residue contaminated by chromium and iron.

PubMed

Brito, Elcia M S; Piñón-Castillo, Hilda A; Guyoneaud, Rémy; Caretta, César A; Gutiérrez-Corona, J Félix; Duran, Robert; Reyna-López, Georgina E; Nevárez-Moorillón, G Virginia; Fahy, Anne; Goñi-Urriza, Marisol

2013-01-01

Anthropogenic extreme environments are among the most interesting sites for the bioprospection of extremophiles since the selection pressures may favor the presence of microorganisms of great interest for taxonomical and astrobiological research as well as for bioremediation technologies and industrial applications. In this work, T-RFLP and 16S rRNA gene library analyses were carried out to describe the autochthonous bacterial populations from an industrial waste characterized as hyper-alkaline (pH between 9 and 14), hyper-saline (around 100 PSU) and highly contaminated with metals, mainly chromium (from 5 to 18 g kg(-1)) and iron (from 2 to 108 g kg(-1)). Due to matrix interference with DNA extraction, a protocol optimization step was required in order to carry out molecular analyses. The most abundant populations, as evaluated by both T-RFLP and 16S rRNA gene library analyses, were affiliated to Bacillus and Lysobacter genera. Lysobacter related sequences were present in the three samples: solid residue and lixiviate sediments from both dry and wet seasons. Sequences related to Thiobacillus were also found; although strains affiliated to this genus are known to have tolerance to metals, they have not previously been detected in alkaline environments. Together with Bacillus (already described as a metal reducer), such organisms could be of use in bioremediation technologies for reducing chromium, as well as for the prospection of enzymes of biotechnological interest.

Transitory microbial habitat in the hyperarid Atacama Desert.

PubMed

Schulze-Makuch, Dirk; Wagner, Dirk; Kounaves, Samuel P; Mangelsdorf, Kai; Devine, Kevin G; de Vera, Jean-Pierre; Schmitt-Kopplin, Philippe; Grossart, Hans-Peter; Parro, Victor; Kaupenjohann, Martin; Galy, Albert; Schneider, Beate; Airo, Alessandro; Frösler, Jan; Davila, Alfonso F; Arens, Felix L; Cáceres, Luis; Cornejo, Francisco Solís; Carrizo, Daniel; Dartnell, Lewis; DiRuggiero, Jocelyne; Flury, Markus; Ganzert, Lars; Gessner, Mark O; Grathwohl, Peter; Guan, Lisa; Heinz, Jacob; Hess, Matthias; Keppler, Frank; Maus, Deborah; McKay, Christopher P; Meckenstock, Rainer U; Montgomery, Wren; Oberlin, Elizabeth A; Probst, Alexander J; Sáenz, Johan S; Sattler, Tobias; Schirmack, Janosch; Sephton, Mark A; Schloter, Michael; Uhl, Jenny; Valenzuela, Bernardita; Vestergaard, Gisle; Wörmer, Lars; Zamorano, Pedro

2018-03-13

Traces of life are nearly ubiquitous on Earth. However, a central unresolved question is whether these traces always indicate an active microbial community or whether, in extreme environments, such as hyperarid deserts, they instead reflect just dormant or dead cells. Although microbial biomass and diversity decrease with increasing aridity in the Atacama Desert, we provide multiple lines of evidence for the presence of an at times metabolically active, microbial community in one of the driest places on Earth. We base this observation on four major lines of evidence: ( i ) a physico-chemical characterization of the soil habitability after an exceptional rain event, ( ii ) identified biomolecules indicative of potentially active cells [e.g., presence of ATP, phospholipid fatty acids (PLFAs), metabolites, and enzymatic activity], ( iii ) measurements of in situ replication rates of genomes of uncultivated bacteria reconstructed from selected samples, and ( iv ) microbial community patterns specific to soil parameters and depths. We infer that the microbial populations have undergone selection and adaptation in response to their specific soil microenvironment and in particular to the degree of aridity. Collectively, our results highlight that even the hyperarid Atacama Desert can provide a habitable environment for microorganisms that allows them to become metabolically active following an episodic increase in moisture and that once it decreases, so does the activity of the microbiota. These results have implications for the prospect of life on other planets such as Mars, which has transitioned from an earlier wetter environment to today's extreme hyperaridity. Copyright © 2018 the Author(s). Published by PNAS.

Measuring the High-Mass IMF in Low-Metallicity Dwarf Galaxies

NASA Astrophysics Data System (ADS)

Weisz, Daniel

2017-08-01

We propose to measure the stellar initial mass function above >1-2 Msun in 46 nearby dwarf galaxies with archival HST observations. This novel approach leverages the redundant age information provided by the main sequence and blue core helium burning stars <500 years old to break the well-known degeneracy between the IMF and star formation history (SFH), enabling a direct measurement of the high-mass IMF in dwarf galaxies. We will be able to constrain the high-mass IMF slope to a precision better than 0.1 to 0.3 dex in each galaxy. Our sample spans a factor of 6 in metallicity ( 5-30% Zsun), 4 decades in star formation rate, and 3 decades in both stellar and gas mass, allowing us to explore the IMF over a wide range of extreme environments.Current observational evidence suggests that nearby dwarf galaxies are the most likely candidates to host significant and systematic variations in the high-mass IMF (e.g., Halpha/UV ratios). However, to date there have been no direct measurements of the high-mass IMF in environments with lower star formation rates and/or more metal poor than the Magellanic Clouds. Our program remedies this shortcoming allowing us to (1) make the first-ever measurement of the high-mass IMF in extremely metal-poor environments; (2) empirically quantify environmental the (lack of) variations in the high-mass IMF; (3) directly test the integrated galactic mass initial mass function (IGIMF), which predicts environmental sensitivity of the IMF in dwarf galaxies.

Transitory microbial habitat in the hyperarid Atacama Desert

NASA Astrophysics Data System (ADS)

Schulze-Makuch, Dirk; Wagner, Dirk; Kounaves, Samuel P.; Mangelsdorf, Kai; Devine, Kevin G.; de Vera, Jean-Pierre; Schmitt-Kopplin, Philippe; Grossart, Hans-Peter; Parro, Victor; Kaupenjohann, Martin; Galy, Albert; Schneider, Beate; Airo, Alessandro; Frösler, Jan; Davila, Alfonso F.; Arens, Felix L.; Cáceres, Luis; Solís Cornejo, Francisco; Carrizo, Daniel; Dartnell, Lewis; DiRuggiero, Jocelyne; Flury, Markus; Ganzert, Lars; Gessner, Mark O.; Grathwohl, Peter; Guan, Lisa; Heinz, Jacob; Hess, Matthias; Keppler, Frank; Maus, Deborah; McKay, Christopher P.; Meckenstock, Rainer U.; Montgomery, Wren; Oberlin, Elizabeth A.; Probst, Alexander J.; Sáenz, Johan S.; Sattler, Tobias; Schirmack, Janosch; Sephton, Mark A.; Schloter, Michael; Uhl, Jenny; Valenzuela, Bernardita; Vestergaard, Gisle; Wörmer, Lars; Zamorano, Pedro

2018-03-01

Traces of life are nearly ubiquitous on Earth. However, a central unresolved question is whether these traces always indicate an active microbial community or whether, in extreme environments, such as hyperarid deserts, they instead reflect just dormant or dead cells. Although microbial biomass and diversity decrease with increasing aridity in the Atacama Desert, we provide multiple lines of evidence for the presence of an at times metabolically active, microbial community in one of the driest places on Earth. We base this observation on four major lines of evidence: (i) a physico-chemical characterization of the soil habitability after an exceptional rain event, (ii) identified biomolecules indicative of potentially active cells [e.g., presence of ATP, phospholipid fatty acids (PLFAs), metabolites, and enzymatic activity], (iii) measurements of in situ replication rates of genomes of uncultivated bacteria reconstructed from selected samples, and (iv) microbial community patterns specific to soil parameters and depths. We infer that the microbial populations have undergone selection and adaptation in response to their specific soil microenvironment and in particular to the degree of aridity. Collectively, our results highlight that even the hyperarid Atacama Desert can provide a habitable environment for microorganisms that allows them to become metabolically active following an episodic increase in moisture and that once it decreases, so does the activity of the microbiota. These results have implications for the prospect of life on other planets such as Mars, which has transitioned from an earlier wetter environment to today’s extreme hyperaridity.

Wireless Sensor Applications in Extreme Aeronautical Environments

NASA Technical Reports Server (NTRS)

Wilson, William C.; Atkinson, Gary M.

2013-01-01

NASA aeronautical programs require rigorous ground and flight testing. Many of the testing environments can be extremely harsh. These environments include cryogenic temperatures and high temperatures (greater than 1500 C). Temperature, pressure, vibration, ionizing radiation, and chemical exposure may all be part of the harsh environment found in testing. This paper presents a survey of research opportunities for universities and industry to develop new wireless sensors that address anticipated structural health monitoring (SHM) and testing needs for aeronautical vehicles. Potential applications of passive wireless sensors for ground testing and high altitude aircraft operations are presented. Some of the challenges and issues of the technology are also presented.

Extreme Environment Basing:Contingency Basing in Dense Urban and Megacity Environments

DTIC Science & Technology

2016-05-24

the placement of a U.S. military contingency base. As stated above, the consideration given to the volume of displaced people caused by the base camp...population density , Dhaka, Bangladesh ........................... 29 B-1 The base camp development planning process...urban areas are not incredibly dense, a great many are. These places represent the extreme end of the urban spectrum for population density and city

Synoptic-scale and mesoscale environments conducive to forest fires during the October 2003 extreme fire event in Southern California

Treesearch

Chenjie Huang; Y.L. Lin; M.L. Kaplan; Joseph J.J. Charney

2009-01-01

This study has employed both observational data and numerical simulation results to diagnose the synoptic-scale and mesoscale environments conducive to forest fires during the October 2003 extreme fire event in southern California. A three-stage process is proposed to illustrate the coupling of the synoptic-scale forcing that is evident from the observations,...

Life in extreme environments: how will humans perform on Mars?

NASA Technical Reports Server (NTRS)

Newman, D. J.

2000-01-01

This review of astronaut extravehicular activity (EVA) and the details of American and Soviet/Russian spacesuit design focuses on design recommendations to enhance astronaut safety and effectiveness. Innovative spacesuit design is essential, given the challenges of future exploration-class missions in which astronauts will be called upon to perform increasingly complex and physically demanding tasks in the extreme environments of microgravity and partial gravity.

40 CFR 355.15 - Which threshold planning quantity do I use for an extremely hazardous substance present at my...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 27 2010-07-01 2010-07-01 false Which threshold planning quantity do I use for an extremely hazardous substance present at my facility in solid form? 355.15 Section 355.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS EMERGENCY...

40 CFR 355.15 - Which threshold planning quantity do I use for an extremely hazardous substance present at my...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 29 2012-07-01 2012-07-01 false Which threshold planning quantity do I use for an extremely hazardous substance present at my facility in solid form? 355.15 Section 355.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS EMERGENCY...

40 CFR 355.15 - Which threshold planning quantity do I use for an extremely hazardous substance present at my...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 28 2011-07-01 2011-07-01 false Which threshold planning quantity do I use for an extremely hazardous substance present at my facility in solid form? 355.15 Section 355.15 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SUPERFUND, EMERGENCY PLANNING, AND COMMUNITY RIGHT-TO-KNOW PROGRAMS EMERGENCY...

Toward Lower Organic Environments in Astromaterial Sample Curation for Diverse Collections

NASA Technical Reports Server (NTRS)

Allton, J. H.; Allen, C. C.; Burkett, P. J.; Calaway, M. J.; Oehler, D. Z.

2012-01-01

Great interest was taken during the frenzied pace of the Apollo lunar sample return to achieve and monitor organic cleanliness. Yet, the first mission resulted in higher organic contamination to samples than desired. But improvements were accomplished by Apollo 12 [1]. Quarantine complicated the goal of achieving organic cleanliness by requiring negative pressure glovebox containment environments, proximity of animal, plant and microbial organic sources, and use of organic sterilants in protocols. A special low organic laboratory was set up at University of California Berkeley (UCB) to cleanly subdivide a subset of samples [2, 3, 4]. Nevertheless, the basic approach of handling rocks and regolith inside of a positive pressure stainless steel glovebox and restrict-ing the tool and container materials allowed in the gloveboxes was established by the last Apollo sample re-turn. In the last 40 years, the collections have grown to encompass Antarctic meteorites, Cosmic Dust, Genesis solar wind, Stardust comet grains and Hayabusa asteroid grains. Each of these collections have unique curation requirements for organic contamination monitor-ing and control. Here is described some changes allowed by improved technology or driven by changes in environmental regulations and economy, concluding with comments on organic witness wafers. Future sample return missions (OSIRIS-Rex; Mars; comets) will require extremely low levels of organic contamination in spacecraft collection and thus similarly low levels in curation. JSC Curation is undertaking a program to document organic baseline levels in current operations and devise ways to reduce those levels.

CVD diamond detectors for ionizing radiation

NASA Astrophysics Data System (ADS)

Friedl, M.; Adam, W.; Bauer, C.; Berdermann, E.; Bergonzo, P.; Bogani, F.; Borchi, E.; Brambilla, A.; Bruzzi, M.; Colledani, C.; Conway, J.; Dabrowski, W.; Delpierre, P.; Deneuville, A.; Dulinski, W.; van Eijk, B.; Fallou, A.; Fizzotti, F.; Foulon, F.; Gan, K. K.; Gheeraert, E.; Grigoriev, E.; Hallewell, G.; Hall-Wilton, R.; Han, S.; Hartjes, F.; Hrubec, J.; Husson, D.; Kagan, H.; Kania, D.; Kaplon, J.; Karl, C.; Kass, R.; Knöpfle, K. T.; Krammer, M.; Logiudice, A.; Lu, R.; Manfredi, P. F.; Manfredotti, C.; Marshall, R. D.; Meier, D.; Mishina, M.; Oh, A.; Pan, L. S.; Palmieri, V. G.; Pernegger, H.; Pernicka, M.; Peitz, A.; Pirollo, S.; Polesello, P.; Pretzl, K.; Re, V.; Riester, J. L.; Roe, S.; Roff, D.; Rudge, A.; Schnetzer, S.; Sciortino, S.; Speziali, V.; Stelzer, H.; Stone, R.; Tapper, R. J.; Tesarek, R.; Thomson, G. B.; Trawick, M.; Trischuk, W.; Vittone, E.; Walsh, A. M.; Wedenig, R.; Weilhammer, P.; Ziock, H.; Zoeller, M.; RD42 Collaboration

1999-10-01

In future HEP accelerators, such as the LHC (CERN), detectors and electronics in the vertex region of the experiments will suffer from extreme radiation. Thus radiation hardness is required for both detectors and electronics to survive in this harsh environment. CVD diamond, which is investigated by the RD42 Collaboration at CERN, can meet these requirements. Samples of up to 2×4 cm2 have been grown and refined for better charge collection properties, which are measured with a β source or in a testbeam. A large number of diamond samples has been irradiated with hadrons to fluences of up to 5×10 15 cm-2 to study the effects of radiation. Both strip and pixel detectors were prepared in various geometries. Samples with strip metallization have been tested with both slow and fast readout electronics, and the first diamond pixel detector proved fully functional with LHC electronics.

Real-time phase evolution of Selective Laser Melted (SLM) Inconel 718 with temperature through synchrotron X-rays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sarley, Brooke A.; Manero, Albert; Cotelo, Jose

2017-01-01

Selective laser melting (SLM) is an additive manufacturing process that uses laser scanning to achieve melting and solidification of a metal powder bed. This process, when applied to develop high temperature material systems, holds great promise for more efficient manufacturing of turbine components that withstand extreme temperatures, heat fluxes, and high mechanical stresses associated with engine environments. These extreme operational conditions demand stringent tolerances and an understanding of the material evolution under thermal loading. This work presents a real-time approach to elucidating the evolution of precipitate phases in SLM Inconel 718 (IN718) under high temperatures using high-energy synchrotron x-ray diffraction.more » Four representative samples (taken along variable build height) were studied in room temperature conditions. Two samples were studied as-processed (samples 1 and 4) and two samples after different thermal treatments (samples 2 and 3). The as-processed samples were found to contain greater amounts of weakening phase, δ. Precipitation hardening of Sample 2 reduced the detectable volume of δ, while also promoting growth of γ00 in the γ matrix. Inversely, solution treatment of Sample 3 produced an overall decrease in precipitate phases. High-temperature, in-situ synchrotron scans during ramp-up, hold, and cool down of two different thermal cycles show the development of precipitate phases. Sample 1 was held at 870°C and subsequently ramped up to 1100°C, during which the high temperature instability of strengthening precipitate, γ00, was seen. γ00 dissolution occurred after 15 minutes at 870°C and was followed by an increase of δ-phase. Sample 4 was held at 800°C and exhibited growth of γ00 after 20 minutes at this temperature. These experiments use in-situ observations to understand the intrinsic thermal effect of the SLM process and the use of heat treatment to manipulate the phase composition of SLM IN718.« less

Editorial: Rich geo- and bio-diversities exist in the South West Atlantic deep-sea: The first human-occupied submersible Shinkai 6500 dive cruise (Iatá-piúna)

NASA Astrophysics Data System (ADS)

Kitazato, Hiroshi; Fujikura, Katsunori; Sumida, Paulo G. Y.; Pellizari, Vivian H.; Perez, Jose Angel

2017-12-01

The deep sea comprises a series of extreme environments, characterized by low temperatures, high hydraulic pressure, the virtual absence of sunlight, and the scarcity of organic nutrients. High hydraulic pressure prevents human access to these environments without using human-occupied submersibles (HOVs), landers or other instruments. Unlike the extreme outer space environments, visited by hundreds of astronauts, only three humans have accessed to the deepest point of the planet.

Exploration of Subglacial Lake Ellsworth

NASA Astrophysics Data System (ADS)

Ross, N.

2012-12-01

Antarctic subglacial lakes are thought to be extreme habitats for microbial life and may contain important records of ice sheet history within their lake-floor sediments. To find if this is true, and to answer the science questions that would follow, direct measurement and sampling of these environments is required. Ever since the water depth of Vostok Subglacial Lake in East Antarctica was shown to be >500 m, attention has been given to how these unique, ancient and pristine subglacial environments may be entered without contamination and adverse disturbance. Several organizations have offered guidelines on the desirable cleanliness and sterility requirements for direct sampling experiments, including the US National Academy of Sciences and the Scientific Committee on Antarctic Research. The aims, design and implementation of subglacial lake access experiments have direct relevance for the exploration of extra-terrestrial ice-covered bodies (e.g. Europa) and the search for microbial life elsewhere in the Solar System. This presentation summarizes the scientific protocols and methods being developed for the exploration of Ellsworth Subglacial Lake in West Antarctica, and provides an up-to-date summary of the status of the project. The proposed exploration, planned for December 2012, involves accessing the lake using a hot-water drill and deploying a sampling probe and sediment corer to allow in situ measurement and sample collection. Details are presented on how this can be undertaken with minimal environmental impact that maximizes scientific return without compromising the environment for future experiments. The implications of this experiment for the search for extra-terrestrial life will be discussed.

Manipulation of Samples at Extreme Temperatures for Fast in-situ Synchrotron Measurements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weber, Richard

An aerodynamic sample levitation system with laser beam heating was integrated with the APS beamlines 6 ID-D, 11 ID-C and 20 BM-B. The new capability enables in-situ measurements of structure and XANES at extreme temperatures (300-3500 °C) and in conditions that completely avoid contact with container surfaces. In addition to maintaining a high degree of sample purity, the use of aerodynamic levitation enables deep supercooling and greatly enhanced glass formation from a wide variety of melts and liquids. Development and integration of controlled extreme sample environments and new measurement techniques is an important aspect of beamline operations and user support.more » Processing and solidifying liquids is a critical value-adding step in manufacturing semiconductors, optical materials, metals and in the operation of many energy conversion devices. Understanding structural evolution is of fundamental importance in condensed materials, geology, and biology. The new capability provides unique possibilities for materials research and helps to develop and maintain a competitive materials manufacturing and energy utilization industry. Test samples were used to demonstrate key features of the capability including experiments on hot crystalline materials, liquids at temperatures from about 500 to 3500 °C. The use of controlled atmospheres using redox gas mixtures enabled in-situ changes in the oxidation states of cations in melts. Significant innovations in this work were: (i) Use of redox gas mixtures to adjust the oxidation state of cations in-situ (ii) Operation with a fully enclosed system suitable for work with nuclear fuel materials (iii) Making high quality high energy in-situ x-ray diffraction measurements (iv) Making high quality in-situ XANES measurements (v) Publishing high impact results (vi) Developing independent funding for the research on nuclear materials This SBIR project work led to a commercial instrument product for the niche market of processing and studying materials in extreme conditions. MDI registered the trademark “Instruments for Innovation®” and sells products under this trademark. SBIR is the ideal vehicle for funding developments such as this since the total market size is relatively small meaning that venture investments to develop products cannot typically be obtained. The sale of niche market instruments for work in extreme conditions has been several million dollars over the last decade. The work to develop and build and sell this instrument has created stable high paying jobs in the technology manufacturing sector. Outreach enabled by this research helped with PhD thesis research, supported three undergraduate interns and one local high school student. In addition, several scientific articles were published, papers were presented at international conferences, and a workshop was held.« less

Energy and water in aestivating amphibians.

PubMed

Carvalho, José E; Navas, Carlos A; Pereira, Isabel C

2010-01-01

The physiological mechanisms, behavioral adjustments, and ecological associations that allow animal species to live in extreme environments have evoked the attention of many zoologists. Often, extreme environments are defined as those believed to be limiting to life in terms of water, energetic availability, and temperature. These three elements seem extreme in a number of arid and semi-arid settings that even so have been colonized by amphibians. Because this taxon is usually seen as the quintessential water-dependent ectotherm tetrapods, their presence in a number of semi-arid environments poses a number of intriguing questions regarding microhabitat choice and physiological plasticity, particularly regarding the ecological and physiological correlates of behaviors granting avoidance of the harshest conditions of semi-arid environments. Such avoidance states, generally associated to the concept of aestivation, are currently seen as a diverse and complex phenomena varying from species to species and involving numerous behavioral and metabolic adjustments that enhance survival during the drought. This chapter reviews the physiological ecology of anuran aestivation, mainly from the perspective of water and energy balance.

Dinocyst taphonomy, impact craters, cyst ghosts, and the Paleocene-Eocene thermal maximum (PETM)

USGS Publications Warehouse

Edwards, Lucy E.

2012-01-01

Dinocysts recovered from sediments related to the Chesapeake Bay impact structure in Virginia and the earliest Eocene suboxic environment in Maryland show strange and intriguing details of preservation. Features such as curled processes, opaque debris, breakage, microborings and cyst ghosts, among others, invite speculation about catastrophic depositional processes, rapid burial and biological and chemical decay. Selected specimens from seven cores taken in the coastal plain of Virginia and Maryland show abnormal preservation features in various combinations that merit illustration, description, discussion and further study. Although the depositional environments described are extreme, many of the features discussed are known from, or could be found in, other environments. These environments will show both similarities to and differences from the extreme environments here.

The operation of 0.35 μm partially depleted SOI CMOS technology in extreme environments

NASA Astrophysics Data System (ADS)

Li, Ying; Niu, Guofu; Cressler, John D.; Patel, Jagdish; Liu, S. T.; Reed, Robert A.; Mojarradi, Mohammad M.; Blalock, Benjamin J.

2003-06-01

We evaluate the usefulness of partially depleted SOI CMOS devices fabricated in a 0.35 μm technology on UNIBOND material for electronics applications requiring robust operation under extreme environment conditions consisting of low and/or high temperature, and under substantial radiation exposure. The threshold voltage, effective mobility, and the impact ionization parameters were determined across temperature for both the nFETs and the pFETs. The radiation response was characterized using threshold voltage shifts of both the front-gate and back-gate transistors. These results suggest that this 0.35 μm partially depleted SOI CMOS technology is suitable for operation across a wide range of extreme environment conditions consisting of: cryogenic temperatures down to 86 K, elevated temperatures up to 573 K, and under radiation exposure to 1.3 Mrad(Si) total dose.

LDEF active optical system components experiment

NASA Technical Reports Server (NTRS)

Blue, M. D.

1992-01-01

A preliminary report on the Active Optical System Components Experiment is presented. This experiment contained 136 components in a six inch deep tray including lasers, infrared detectors and arrays, ultraviolet light detectors, light-emitting diodes, a light modulator, flash lamps, optical filters, glasses, and samples of surface finishes. Thermal, mechanical, and structural considerations leading to the design of the tray hardware are discussed. In general, changes in the retested component characteristics appear as much related to the passage of time as to the effects of the space environment, but organic materials, multilayer optical interference filters, and extreme-infrared reflectivity of black paints show unexpected changes.

PAME Proceedings, Pattern Analysis in the Marine Environment, an Ocean Science and Technology Workshop Held at the Naval Ocean Research and Development Activity, NSTL, MS. on 24-26 March 1982.

DTIC Science & Technology

1983-10-01

adjustable (T1 to T2 ), and nonadjusted (less than T2 ) . Nonadjusted loadings are ones that correspond to "outliers" that will not affect ...specified distance are considered ,’ ’. outliers or impurities and will not affect the overall solution. "Distance" in this sense represents the...raises the " ". " ~* possiblity that a relatively few isolated data points might seriously affect the relative geometry of extreme samples with regard to

Instrument developments for chemical and physical characterization, mapping and sampling of extreme environments (Antarctic sub ice environment)

NASA Astrophysics Data System (ADS)

Vogel, S. W.; Powell, R. D.; Griffith, I.; Lawson, T.; Schiraga, S.; Ludlam, G.; Oen, J.

2009-12-01

A number of instrumentation is currently under development designed to enable the study of subglacial environments in Antarctica through narrow kilometer long boreholes. Instrumentation includes: - slim line Sub-Ice ROV (SIR), - Geochemical Instrumentation Package for Sub Ice Environments (GIPSIE) to study geochemical fluxes in water and across the sediment water interface (CO2, CH4, dO, NH4, NO3, Si, PO4, pH, redox, T, H2, HS, O2, N2O, CTD, particle size, turbidity, color camera, current meter and automated water sampler) with real-time telemetry for targeted sampling, - long term energy-balance mooring system, - active source slide hammer sediment corer, and - integration of a current sensor into the ITP profiler. The instrumentation design is modular and suitable for remote operated as well as autonomous long-term deployment. Of interest to the broader science community is the development of the GIPSIE and efforts to document the effect of sample recovery from depth on the sample chemistry. The GIPSIE is a geochemical instrumentation package with life stream telemetry, allowing for user controlled targeted sampling of water column and the water sediment interphase for chemical and biological work based on actual measurements and not a preprogrammed automated system. The porewater profiler (pH, redox, T, H2, HS, O2, N2O) can penetrate the upper 50 cm of sediment and penetration is documented with real time video. Associated with GIPSIE is an on-site lab set-up, utilizing a set of identical sensors. Comparison between the insitu measurements and measurements taken onsite directly after samples are recovered from depth permits assessing the effect of sample recovery on water and sediment core chemistry. Sample recovery related changes are mainly caused by changes in the pressure temperature field and exposure of samples to atmospheric conditions. Exposure of anaerobic samples to oxygen is here a specific concern. Recovery from depth effects in generally pH, solubility of gases and nutrients and can initiate complex chemical reaction, the product of which is later measured in the lab. Further information on the instrument developments can be found at http://jove.geol.niu.edu/faculty/svogel/Technology/Technology-index.html

Ecologic Niche Modeling of Blastomyces dermatitidis in Wisconsin

PubMed Central

Reed, Kurt D.; Meece, Jennifer K.; Archer, John R.; Peterson, A. Townsend

2008-01-01

Background Blastomycosis is a potentially fatal mycosis that is acquired by inhaling infectious spores of Blastomyces dermatitidis present in the environment. The ecology of this pathogen is poorly understood, in part because it has been extremely difficult to identify the niche(s) it occupies based on culture isolation of the organism from environmental samples. Methodology/Principal Findings We investigated the ecology of blastomycosis by performing maximum entropy modeling of exposure sites from 156 cases of human and canine blastomycosis to provide a regional-scale perspective of the geographic and ecologic distribution of B. dermatitidis in Wisconsin. Based on analysis with climatic, topographic, surface reflectance and other environmental variables, we predicted that ecologic conditions favorable for maintaining the fungus in nature occur predominantly within northern counties and counties along the western shoreline of Lake Michigan. Areas of highest predicted occurrence were often in proximity to waterways, especially in northcentral Wisconsin, where incidence of infection is highest. Ecologic conditions suitable for B. dermatitidis are present in urban and rural environments, and may differ at the extremes of distribution of the species in the state. Conclusions/Significance Our results provide a framework for a more informed search for specific environmental factors modulating B. dermatitidis occurrence and transmission and will be useful for improving public health awareness of relative exposure risks. PMID:18446224

Aerosol sampling system for collection of Capstone depleted uranium particles in a high-energy environment.

PubMed

Holmes, Thomas D; Guilmette, Raymond A; Cheng, Yung Sung; Parkhurst, Mary Ann; Hoover, Mark D

2009-03-01

The Capstone Depleted Uranium (DU) Aerosol Study was undertaken to obtain aerosol samples resulting from a large-caliber DU penetrator striking an Abrams or Bradley test vehicle. The sampling strategy was designed to (1) optimize the performance of the samplers and maintain their integrity in the extreme environment created during perforation of an armored vehicle by a DU penetrator, (2) collect aerosols as a function of time post perforation, and (3) obtain size-classified samples for analysis of chemical composition, particle morphology, and solubility in lung fluid. This paper describes the experimental setup and sampling methodologies used to achieve these objectives. Custom-designed arrays of sampling heads were secured to the inside of the target in locations approximating the breathing zones of the crew locations in the test vehicles. Each array was designed to support nine filter cassettes and nine cascade impactors mounted with quick-disconnect fittings. Shielding and sampler placement strategies were used to minimize sampler loss caused by the penetrator impact and the resulting fragments of eroded penetrator and perforated armor. A cyclone train was used to collect larger quantities of DU aerosol for measurement of chemical composition and solubility. A moving filter sample was used to obtain semicontinuous samples for DU concentration determination. Control for the air samplers was provided by five remotely located valve control and pressure monitoring units located inside and around the test vehicle. These units were connected to a computer interface chassis and controlled using a customized LabVIEW engineering computer control program. The aerosol sampling arrays and control systems for the Capstone study provided the needed aerosol samples for physicochemical analysis, and the resultant data were used for risk assessment of exposure to DU aerosol.

Aerosol Sampling System for Collection of Capstone Depleted Uranium Particles in a High-Energy Environment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Holmes, Thomas D.; Guilmette, Raymond A.; Cheng, Yung-Sung

2009-03-01

The Capstone Depleted Uranium Aerosol Study was undertaken to obtain aerosol samples resulting from a kinetic-energy cartridge with a large-caliber depleted uranium (DU) penetrator striking an Abrams or Bradley test vehicle. The sampling strategy was designed to (1) optimize the performance of the samplers and maintain their integrity in the extreme environment created during perforation of an armored vehicle by a DU penetrator, (2) collect aerosols as a function of time post-impact, and (3) obtain size-classified samples for analysis of chemical composition, particle morphology, and solubility in lung fluid. This paper describes the experimental setup and sampling methodologies used tomore » achieve these objectives. Custom-designed arrays of sampling heads were secured to the inside of the target in locations approximating the breathing zones of the vehicle commander, loader, gunner, and driver. Each array was designed to support nine filter cassettes and nine cascade impactors mounted with quick-disconnect fittings. Shielding and sampler placement strategies were used to minimize sampler loss caused by the penetrator impact and the resulting fragments of eroded penetrator and perforated armor. A cyclone train was used to collect larger quantities of DU aerosol for chemical composition and solubility. A moving filter sample was used to obtain semicontinuous samples for depleted uranium concentration determination. Control for the air samplers was provided by five remotely located valve control and pressure monitoring units located inside and around the test vehicle. These units were connected to a computer interface chassis and controlled using a customized LabVIEW engineering computer control program. The aerosol sampling arrays and control systems for the Capstone study provided the needed aerosol samples for physicochemical analysis, and the resultant data were used for risk assessment of exposure to DU aerosol.« less

Microbiome composition and geochemical characteristics of deep subsurface high-pressure environment, Pyhäsalmi mine Finland

PubMed Central

Miettinen, Hanna; Kietäväinen, Riikka; Sohlberg, Elina; Numminen, Mikko; Ahonen, Lasse; Itävaara, Merja

2015-01-01

Pyhäsalmi mine in central Finland provides an excellent opportunity to study microbial and geochemical processes in a deep subsurface crystalline rock environment through near-vertical drill holes that reach to a depth of more than two kilometers below the surface. However, microbial sampling was challenging in this high-pressure environment. Nucleic acid yields obtained were extremely low when compared to the cell counts detected (1.4 × 104 cells mL−1) in water. The water for nucleic acid analysis went through high decompression (60–130 bar) during sampling, whereas water samples for detection of cell counts by microscopy could be collected with slow decompression. No clear cells could be identified in water that went through high decompression. The high-pressure decompression may have damaged part of the cells and the nucleic acids escaped through the filter. The microbial diversity was analyzed from two drill holes by pyrosequencing amplicons of the bacterial and archaeal 16S rRNA genes and from the fungal ITS regions from both DNA and RNA fractions. The identified prokaryotic diversity was low, dominated by Firmicute, Beta- and Gammaproteobacteria species that are common in deep subsurface environments. The archaeal diversity consisted mainly of Methanobacteriales. Ascomycota dominated the fungal diversity and fungi were discovered to be active and to produce ribosomes in the deep oligotrophic biosphere. The deep fluids from the Pyhäsalmi mine shared several features with other deep Precambrian continental subsurface environments including saline, Ca-dominated water and stable isotope compositions positioning left from the meteoric water line. The dissolved gas phase was dominated by nitrogen but the gas composition clearly differed from that of atmospheric air. Despite carbon-poor conditions indicated by the lack of carbon-rich fracture fillings and only minor amounts of dissolved carbon detected in formation waters, some methane was found in the drill holes. No dramatic differences in gas compositions were observed between different gas sampling methods tested. For simple characterization of gas composition the most convenient way to collect samples is from free flowing fluid. However, compared to a pressurized method a relative decrease in the least soluble gases may appear. PMID:26579109

The Extreme Hosts of Extreme Supernovae

NASA Astrophysics Data System (ADS)

Neill, James D.

2012-01-01

We present the results from a deeper survey of Luminous Supernova (LSN) hosts with the Galaxy Evolution Explorer (GALEX). We have added new, multiple kilo-second observations to our original observations of seventeen LSN hosts providing better constraints on their physical properties. We place the LSNe hosts on the galaxy NUV-r versus M(r) color magnitude diagram (CMD) with a larger comparison sample ( 26,000) to illustrate the extreme nature of these galaxies. The LSN hosts favor low-density regions of the galaxy CMD falling on the blue edge of the blue cloud toward the low luminosity end. The new observations provide tighter constraints on the star formation rates (SFRs) and stellar masses, M(*), and show that the LSNe result from regions of high specific star formation and yet low total SFR. This regime is of particular interest for exploring the upper end of the stellar IMF and its variation. If our understanding of the progenitors of the LSNe leans toward very massive (> 200 M_sun) progenitors, the potential for a conflict with IMF theory exists because the conditions found in the hosts producing the LSNe should not create such massive stars. If it also required that LSNe can only be produced in primordial or very low metallicity environments, then they will also provide evidence for strong variation in metallicity within a dwarf galaxy, since their masses are consistent with low, but not extreme metallicity.

Properties of Cathodoluminescence for Cryogenic Applications of SiO2-based Space Observatory Optics and Coatings

NASA Technical Reports Server (NTRS)

Evans, Amberly; Dennison, J.R.; Wilson, Gregory; Dekany, Justin; Bowers Charles W.; Meloy, Robert; Heaney, James B.

2013-01-01

Disordered thin film SiO2SiOx coatings undergoing electron-beam bombardment exhibit cathodoluminescence, which can produce deleterious stray background light in cryogenic space-based astronomical observatories exposed to high-energy electron fluxes from space plasmas. As future observatory missions push the envelope into more extreme environments and more complex and sensitive detection, a fundamental understanding of the dependencies of this cathodoluminescence becomes critical to meet performance objectives of these advanced space-based observatories. Measurements of absolute radiance and emission spectra as functions of incident electron energy, flux, and power typical of space environments are presented for thin (60-200 nm) SiO2SiOx optical coatings on reflective metal substrates over a range of sample temperatures (40-400 K) and emission wavelengths (260-5000 nm). Luminescent intensity and peak wavelengths of four distinct bands were observed in UVVISNIR emission spectra, ranging from 300 nm to 1000 nm. A simple model is proposed that describes the dependence of cathodoluminescence on irradiation time, incident flux and energy, sample thickness, and temperature.

Sulfur "Concrete" for Lunar Applications - Sublimation Concerns

NASA Technical Reports Server (NTRS)

Grugel, Richard N.; Toutanji, Houssam

2006-01-01

Melting sulfur and mixing it with an aggregate to form "concrete" is commercially well established and constitutes a material that is particularly well-suited for use in corrosive environments. Discovery of the mineral troilite (FeS) on the moon poses the question of extracting the sulfur for use as a lunar construction material. This would be an attractive alternative to conventional concrete as it does not require water. However, the viability of sulfur concrete in a lunar environment, which is characterized by lack of an atmosphere and extreme temperatures, is not well understood. Here it is assumed that the lunar ore can be mined, refined, and the raw sulfur melded with appropriate lunar regolith to form, for example, bricks. This study evaluates pure sulfur and two sets of small sulfur concrete samples that have been prepared using JSC-1 lunar stimulant and SiO2 powder as aggregate additions. Each set was subjected to extended periods in a vacuum environment to evaluate sublimation issues. Results from these experiments are presented and discussed within the context of the lunar environment.

Containerless processing of single crystals in low-G environment

NASA Technical Reports Server (NTRS)

Walter, H. U.

1974-01-01

Experiments on containerless crystal growth from the melt were conducted during Skylab missions SL3 and SL4 (Skylab Experiment M-560). Six samples of InSb were processed, one of them heavily doped with selenium. The concept of the experiment is discussed and related to general crystal growth methods and their merits as techniques for containerless processing in space. The morphology of the crystals obtained is explained in terms of volume changes associated with solidification and wetting conditions during solidification. All samples exhibit extremely well developed growth facets. Analysis by X-ray topographical methods and chemical etching shows that the crystals are of high structural perfection. Average dislocation density as revealed by etching is of the order of 100 per sq cm; no dislocation clusters could be observed in the space-grown samples. A sequence of striations that is observed in the first half of the selenium-doped sample is explained as being caused by periodic surface breakdown.

Martian Magmatic-Driven Hydrothermal Sites: Potential Sources of Energy, Water, and Life

NASA Technical Reports Server (NTRS)

Anderson, R. C.; Dohm, J. M.; Baker, V. R.; Ferris, J. C.; Hare, T. M.; Tanaka, K. L.; Klemaszewski, J. E.; Skinner, J. A.; Scott, D. H.

2000-01-01

Magmatic-driven processes and impact events dominate the geologic record of Mars. Such recorded geologic activity coupled with significant evidence of past and present-day water/ice, above and below the martian surface, indicate that hydrothermal environments certainly existed in the past and may exist today. The identification of such environments, especially long-lived magmatic-driven hydrothermal environments, provides NASA with significant target sites for future sample return missions, since they (1) could favor the development and sustenance of life, (2) may comprise a large variety of exotic mineral assemblages, and (3) could potentially contain water/ice reservoirs for future Mars-related human activities. If life developed on Mars, the fossil record would presumably be at its greatest concentration and diversity in environments where long-term energy sources and water coexisted such as at sites where long-lived, magmatic-driven hydrothermal activity occurred. These assertions are supported by terrestrial analogs. Small, single-celled creatures (prokaryotes) are vitally important in the evolution of the Earth; these prokaryotes are environmentally tough and tolerant of environmental extremes of pH, temperature, salinity, and anoxic conditions found around hydrothermal vents. In addition, there is a great ability for bacteria to survive long periods of geologic time in extreme conditions, including high temperature hydrogen sulfide and sulfur erupted from Mount St. Helens volcano. Our team of investigators is conducting a geological investigation using multiple mission-derived datasets (e.g., existing geologic map data, MOC imagery, MOLA, TES image data, geophysical data, etc.) to identify prime target sites of hydrothermal activity for future hydrological, mineralogical, and biological investigations. The identification of these sites will enhance the probability of success for future missions to Mars.

Microbes in subglacial environments: Significant biogeochemical agents?

NASA Astrophysics Data System (ADS)

Lanoil, B.; Gaidos, E.; Anderson, S.

2003-04-01

Recent studies have demonstrated the presence of abundant microbes in several subglacial environments, including alpine and polar glaciers and the giant Antarctic subglacial lake, Lake Vostok. Some indirect isotopic and geochemical evidence indicate that microbial communities may be active in these cold, dark, extreme environments. We have been using molecular biology, microbiology, and geochemistry tools to correlate the identity of microbes in subglacial systems with important geochemical parameters. Our studies have focused on several sites, including a subglacial volcanic caldera lake in Iceland (Grímsvötn; GI), a temperate alpine valley glacier in Alaska (Bench Glacier; BG), and a polythermal Arctic valley glacier in Nunavut, Canada (John Evans Glacier; JEG). Our preliminary data indicate the presence of some similar microbial groups in BG and JEG, perhaps reflecting a selection for organisms which are capable of growth under extreme physical conditions. However, there is also a large fraction of the communities which differ between the Alaskan and Canadian sites. The predicted physiologies of the variable community components appear to correlate well with the geochemistry of the BG and JEG. We have also detected C-fixation and heterotrophic activities at near in situ conditions in intact samples and/or in bacteria isolated from all three sites. Furthermore, subglacial pelagic and sediment-attached microbial communities at GI are significantly different than snow or ice communities, indicating that the subglacial community may be endemic to the caldera lake. Based on these data, we predict that microbes play important roles in chemical weathering processes, organic carbon turnover, and other (bio)geochemical processes in subglacial environments. Our results may have important implications for biogeochemical cycles, especially during periods in earth history when there was significant ice cover, e.g. the Quaternary and Neoproterozoic “Snowball Earth” events and may provide insights into habitats on other planets.

Extreme-Environment Silicon-Carbide (SiC) Wireless Sensor Suite

NASA Technical Reports Server (NTRS)

Yang, Jie

2015-01-01

Phase II objectives: Develop an integrated silicon-carbide wireless sensor suite capable of in situ measurements of critical characteristics of NTP engine; Compose silicon-carbide wireless sensor suite of: Extreme-environment sensors center, Dedicated high-temperature (450 deg C) silicon-carbide electronics that provide power and signal conditioning capabilities as well as radio frequency modulation and wireless data transmission capabilities center, An onboard energy harvesting system as a power source.

Environmental hazards, hot, cold, altitude, and sun.

PubMed

Dhillon, Sundeep

2012-09-01

There has been an increase in both recreational and adventure travel to extreme environments. Humans can successfully acclimatize to and perform reasonably well in extreme environments, provided that sufficient time is given for acclimatization (where possible) and that they use appropriate behavior. This is aided by a knowledge of the problems likely to be encountered and their prevention, recognition, and treatment. Crown Copyright © 2012. Published by Elsevier Inc. All rights reserved.

Distributed Motor Controller (DMC) for Operation in Extreme Environments

NASA Technical Reports Server (NTRS)

McKinney, Colin M.; Yager, Jeremy A.; Mojarradi, Mohammad M.; Some, Rafi; Sirota, Allen; Kopf, Ted; Stern, Ryan; Hunter, Don

2012-01-01

This paper presents an extreme environment capable Distributed Motor Controller (DMC) module suitable for operation with a distributed architecture of future spacecraft systems. This motor controller is designed to be a bus-based electronics module capable of operating a single Brushless DC motor in extreme space environments: temperature (-120 C to +85 C required, -180 C to +100 C stretch goal); radiation (>;20K required, >;100KRad stretch goal); >;360 cycles of operation. Achieving this objective will result in a scalable modular configuration for motor control with enhanced reliability that will greatly lower cost during the design, fabrication and ATLO phases of future missions. Within the heart of the DMC lies a pair of cold-capable Application Specific Integrated Circuits (ASICs) and a Field Programmable Gate Array (FPGA) that enable its miniaturization and operation in extreme environments. The ASICs are fabricated in the IBM 0.5 micron Silicon Germanium (SiGe) BiCMOS process and are comprised of Analog circuitry to provide telemetry information, sensor interface, and health and status of DMC. The FPGA contains logic to provide motor control, status monitoring and spacecraft interface. The testing and characterization of these ASICs have yielded excellent functionality in cold temperatures (-135 C). The DMC module has demonstrated successful operation of a motor at temperature.

Long-term effects of peer victimization on social outcomes through the fourth decade of life in individuals born at normal or extremely low birthweight.

PubMed

Day, Kimberly L; Van Lieshout, Ryan J; Vaillancourt, Tracy; Saigal, Saroj; Boyle, Michael H; Schmidt, Louis A

2017-09-01

Exposure to early adversity is known to have deleterious effects on brain-behaviour relations across the lifespan and across a range of domains. Here, we tested a cumulative risk hypothesis of adult social functioning and health outcomes in the fourth decade of life, using the oldest known longitudinally followed cohort of survivors of extremely low birthweight (ELBW; <1,000 g). We investigated the additional impact of peer victimization in youth on social outcomes at age 29-36 years in ELBW survivors and matched normal birthweight (NBW; >2,500 g) participants. In the combined sample, peer victimization was associated with lower likelihood of having children and household income, poorer family functioning and self-esteem, more loneliness and chronic health conditions, less social support, and increased likelihood for contact with police. Moderation analyses indicated that among ELBW survivors, compared to their NBW counterparts, victimization was more strongly associated with being convicted of a crime and with having chronic health conditions. These findings highlight the negative long-term impact of peer victimization on all children and that some outcomes may be differentially affected by prenatal and early post-natal environments. Statement of contribution What is already known on this subject Exposure to early adversity has deleterious effects on brain-behaviour relations across the lifespan. Extremely premature children have higher rates of exposure to adversities, including peer victimization. Peer victimization is associated with adverse outcomes in adulthood in those born at term. What does this study add? Victimization negatively impacts the social outcomes of those born extremely premature and at term into adulthood. Associations appear to be affected by individual differences in prenatal and early post-natal environments. Intervention is crucial when peer victimization occurs in children at risk, as well as those typically developing. © 2016 The British Psychological Society.

Extreme Science (LBNL Science at the Theater)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ajo-Franklin, Caroline; Klein, Spencer; Minor, Andrew

On Feb. 27, 2012 at the Berkeley Repertory Theatre, four Berkeley Lab scientists presented talks related to extreme science - and what it means to you. Topics include: Neutrino hunting in Antarctica. Learn why Spencer Klein goes to the ends of the Earth to search for these ghostly particles. From Chernobyl to Central Asia, Tamas Torok travels the globe to study microbial diversity in extreme environments. Andrew Minor uses the world's most advanced electron microscopes to explore materials at ultrahigh stresses and in harsh environments. And microbes that talk to computers? Caroline Ajo-Franklin is pioneering cellular-electrical connections that could helpmore » transform sunlight into fuel.« less

Terrestrial Applications of Extreme Environment Stirling Space Power Systems

NASA Technical Reports Server (NTRS)

Dyson, Rodger. W.

2012-01-01

NASA has been developing power systems capable of long-term operation in extreme environments such as the surface of Venus. This technology can use any external heat source to efficiently provide electrical power and cooling; and it is designed to be extremely efficient and reliable for extended space missions. Terrestrial applications include: use in electric hybrid vehicles; distributed home co-generation/cooling; and quiet recreational vehicle power generation. This technology can reduce environmental emissions, petroleum consumption, and noise while eliminating maintenance and environmental damage from automotive fluids such as oil lubricants and air conditioning coolant. This report will provide an overview of this new technology and its applications.

Microbial Diversity in Extreme Marine Habitats and Their Biomolecules

PubMed Central

Poli, Annarita; Finore, Ilaria; Romano, Ida; Gioiello, Alessia; Lama, Licia; Nicolaus, Barbara

2017-01-01

Extreme marine environments have been the subject of many studies and scientific publications. For many years, these environmental niches, which are characterized by high or low temperatures, high-pressure, low pH, high salt concentrations and also two or more extreme parameters in combination, have been thought to be incompatible to any life forms. Thanks to new technologies such as metagenomics, it is now possible to detect life in most extreme environments. Starting from the discovery of deep sea hydrothermal vents up to the study of marine biodiversity, new microorganisms have been identified, and their potential uses in several applied fields have been outlined. Thermophile, halophile, alkalophile, psychrophile, piezophile and polyextremophile microorganisms have been isolated from these marine environments; they proliferate thanks to adaptation strategies involving diverse cellular metabolic mechanisms. Therefore, a vast number of new biomolecules such as enzymes, polymers and osmolytes from the inhabitant microbial community of the sea have been studied, and there is a growing interest in the potential returns of several industrial production processes concerning the pharmaceutical, medical, environmental and food fields. PMID:28509857

Localized corrosion of high performance metal alloys in an acid/salt environment

NASA Technical Reports Server (NTRS)

Macdowell, L. G.; Ontiveros, C.

1991-01-01

Various vacuum jacketed cryogenic supply lines at the Space Shuttle launch site at Kennedy Space Center use convoluted flexible expansion joints. The atmosphere at the launch site has a very high salt content, and during a launch, fuel combustion products include hydrochloric acid. This extremely corrosive environment has caused pitting corrosion failure in the thin walled 304L stainless steel flex hoses. A search was done to find a more corrosion resistant replacement material. The study focussed on 19 metal alloys. Tests which were performed include electrochemical corrosion testing, accelerated corrosion testing in a salt fog chamber, and long term exposure at a beach corrosion testing site. Based on the results of these tests, several nickel based alloys were found to have very high resistance to this corrosive environment. Also, there was excellent agreement between the electrochemical tests and the actual beach exposure tests. This suggests that electrochemical testing may be useful for narrowing the field of potential candidate alloys before subjecting samples to long term beach exposure.

Characterization of the Martian magnetic topology response to extreme solar transient events with MGS data

NASA Astrophysics Data System (ADS)

Xu, S.; Curry, S.; Mitchell, D. L.; Luhmann, J. G.; Lillis, R. J.; Dong, C.

2017-12-01

Characterizing how the solar cycle affects the physics of the Mars-solar wind interaction can improve our understanding of Mars' atmospheric evolution and the plasma environment at Mars. In particular, solar transient events such as Interplanetary Coronal Mass Ejections (ICMEs) and Stream Interaction Regions (SIRs) significantly change the solar-wind interaction, including the magnetic topology and ion acceleration. However, both the Mars Express and Mars Atmosphere Volatile EvolutioN (MAVEN) missions have encountered relatively few extreme solar transient events due to the recent low solar activity (2004-2017). In contrast, Mars Global Surveyor (MGS) was operating during a relatively active solar maximum (1999-2003). Based on new results from MAVEN, this study reanalyzes MGS data to better understand how the Martian plasma environment responds to extreme solar events. In particular, we aim to investigate how the magnetic topology during these extreme events differs from the topology during quiet times. We conduct orbit comparisons of the magnetic topology inferred from MGS electron pitch angle distributions during quiet periods and extreme events to determine how the open and closed field patterns respond to extreme events.

A comparison between measured surface microtopography and observed scattering in the extreme ultraviolet

NASA Technical Reports Server (NTRS)

Green, James; Jelinsky, Sharon; Bowyer, Stuart; Malina, Roger F.

1988-01-01

The paper presents comparative measurements of surface roughness on prepared samples. These measurements have been made with both Talystep profilometers and WYKO interferometers. In addition, the scattering distribution from these samples was measured at extreme ultraviolet wavelengths. The utility of the WYKO interferometer and Talystep device for specifying extreme ultraviolet mirror surface quality is discussed.

The Behavior of Hydrogen Under Extreme Conditions on Ultrafast Timescales (A "Life at the Frontiers of Energy Research" contest entry from the 2011 Energy Frontier Research Centers (EFRCs) Summit and Forum)

ScienceCinema

Mao, Ho-kwang (Director, Center for Energy Frontier Research in Extreme Environments); EFree Staff

2017-12-09

'The Behavior of Hydrogen Under Extreme Conditions on Ultrafast Timescales ' was submitted by the Center for Energy Frontier Research in Extreme Environments (EFree) to the 'Life at the Frontiers of Energy Research' video contest at the 2011 Science for Our Nation's Energy Future: Energy Frontier Research Centers (EFRCs) Summit and Forum. Twenty-six EFRCs created short videos to highlight their mission and their work. EFree is directed by Ho-kwang Mao at the Carnegie Institute of Washington and is a partnership of scientists from thirteen institutions.The Office of Basic Energy Sciences in the U.S. Department of Energy's Office of Science established the 46 Energy Frontier Research Centers (EFRCs) in 2009. These collaboratively-organized centers conduct fundamental research focused on 'grand challenges' and use-inspired 'basic research needs' recently identified in major strategic planning efforts by the scientific community. The overall purpose is to accelerate scientific progress toward meeting the nation's critical energy challenges. The mission of Energy Frontier Research in Extreme Environments is 'to accelerate the discovery and creation of energy-relevant materials using extreme pressures and temperatures.' Research topics are: catalysis (CO{sub 2}, water), photocatalysis, solid state lighting, optics, thermelectric, phonons, thermal conductivity, solar electrodes, fuel cells, superconductivity, extreme environment, radiation effects, defects, spin dynamics, CO{sub 2} (capture, convert, store), greenhouse gas, hydrogen (fuel, storage), ultrafast physics, novel materials synthesis, and defect tolerant materials.

Orbital Electron Capture Rates in Extreme Astrophysical Environments

NASA Astrophysics Data System (ADS)

Martin, Matthew; McDonald, William; Leach, Kyle

2017-09-01

In an attempt to better understand EC decay rates in hot environments, we have developed a program to examine and parse all evaluated atomic and nuclear data. Taking into account the effects of ionization on accessible decay states and electron capture probabilities, half lives across the nuclear chart can be investigated without the need for theoretical estimates. Part of the ongoing project will include isolating stable isotopes that become unstable due to ionization and estimating their stability in these new environments. In addition, we hope to account for a thermal population of excited states to better simulate these environments. This should aide in the complete understanding of nuclear processes in these extreme astrophysical environments. This work is supported in part by the U.S. Department of Energy, Office of Science, Office of Nuclear Physics.

Diamond-Dispersed Fiber-Reinforced Composite for Superior Friction and Wear Properties in Extreme Environments and Method for Fabricating the Same

NASA Technical Reports Server (NTRS)

Voronov, Oleg A (Inventor); Street, Kenneth (Inventor); Kear, Bernard H (Inventor)

2017-01-01

Systems, methods, and articles of manufacture related to composite materials are discussed herein. These materials can be based on a mixture of diamond particles with a matrix and fibers or fabrics. The matrix can be formed into the composite material through optional pressurization and via heat treatment. These materials display exceptionally low friction coefficient and superior wear resistance in extreme environments.

Solar Power Generation in Extreme Space Environments

NASA Technical Reports Server (NTRS)

Elliott, Frederick W.; Piszczor, Michael F.

2016-01-01

The exploration of space requires power for guidance, navigation, and control; instrumentation; thermal control; communications and data handling; and many subsystems and activities. Generating sufficient and reliable power in deep space through the use of solar arrays becomes even more challenging as solar intensity decreases and high radiation levels begin to degrade the performance of photovoltaic devices. The Extreme Environments Solar Power (EESP) project goal is to develop advanced photovoltaic technology to address these challenges.

Glenn Extreme Environments Rig (GEER) Independent Review

NASA Technical Reports Server (NTRS)

Jankovsky, Robert S.; Smiles, Michael D.; George, Mark A.; Ton, Mimi C.; Le, Son K.

2015-01-01

The Chief of the Space Science Project Office at Glenn Research Center (GRC) requested support from the NASA Engineering and Safety Center (NESC) to satisfy a request from the Science Mission Directorate (SMD) Associate Administrator and the Planetary Science Division Chief to obtain an independent review of the Glenn Extreme Environments Rig (GEER) and the operational controls in place for mitigating any hazard associated with its operation. This document contains the outcome of the NESC assessment.

Relationship of psychological and physiological parameters during an arctic ski expedition

NASA Astrophysics Data System (ADS)

Bishop, Sheryl L.; Grobler, Lukas C.; SchjØll, Olaf

2001-08-01

Considerable data (primarily physiological) have been collected during expeditions in extreme environments over the last century. Physiological measurements have only recently been examined in association with the emotional or behavioral state of the subject. Establishing this psychophysiological relationship is essential to understanding fully the adaptation of humans to the stresses of extreme environments. This pilot study investigated the simultaneous collection of physiological, psychological and behavioral data from a two-man Greenland expedition in order to model how specific relationships between physiological and psychological adaptation to a polar environment may be identified. The data collected describes changes in adrenal and other hormonal activity and psychological functioning. Levels of cortisol and testosterone were calculated. Factors influencing the plasma profiles of the aforementioned included 24-hour sunlight, high calorific intake of more than 28 000 kJ/day and extreme physical exercise. There was a difference between individual psychological profiles as well as self-report stress and physiological stress.

A low cost, high precision extreme/harsh cold environment, autonomous sensor data gathering and transmission platform.

NASA Astrophysics Data System (ADS)

Chetty, S.; Field, L. A.

2014-12-01

SWIMS III, is a low cost, autonomous sensor data gathering platform developed specifically for extreme/harsh cold environments. Arctic ocean's continuing decrease of summer-time ice is related to rapidly diminishing multi-year ice due to the effects of climate change. Ice911 Research aims to develop environmentally inert materials that when deployed will increase the albedo, enabling the formation and/preservation of multi-year ice. SWIMS III's sophisticated autonomous sensors are designed to measure the albedo, weather, water temperature and other environmental parameters. This platform uses low cost, high accuracy/precision sensors, extreme environment command and data handling computer system using satellite and terrestrial wireless solution. The system also incorporates tilt sensors and sonar based ice thickness sensors. The system is light weight and can be deployed by hand by a single person. This presentation covers the technical, and design challenges in developing and deploying these platforms.

The Mars Simulation Laboratory, University of Aarhus

NASA Astrophysics Data System (ADS)

Merrison, J. P.; Field, D.; Finster, K.; Lomstein, B. Aa.; Nørnberg, P.; Ramsing, N. B.; Uggerhøj, E.

2001-08-01

Present day Mars presents an extremely hostile environment to organic material. The average temperature is low (-50C), the atmospheric pressure is also low (7mbar) and there is little water over most of the planet. Chemically the surface is extremely oxidising and no signs of organic material have been detected. There is also a strong component of ultra violet radiation in the Martian sun light, lethal to most organisms. At Aarhus University we have constructed a Mars simulation environment which reproduces the physical, chemical and mineralogical conditions on Mars. It is hoped to set limits on where organic matter (or even life) might exist on Mars, for example at some depth under the surface, beneath the polar ice or within rocks. It is also possible to adjust the conditions in the simulation to investigate the most extreme environments in which organisms can be preserved or still function.

Coexistence of Lactic Acid Bacteria and Potential Spoilage Microbiota in a Dairy Processing Environment

PubMed Central

Stellato, Giuseppina; De Filippis, Francesca; La Storia, Antonietta

2015-01-01

Microbial contamination in food processing plants can play a fundamental role in food quality and safety. In this study, the microbiota in a dairy plant was studied by both 16S rRNA- and 26S rRNA-based culture-independent high-throughput amplicon sequencing. Environmental samples from surfaces and tools were studied along with the different types of cheese produced in the same plant. The microbiota of environmental swabs was very complex, including more than 200 operational taxonomic units with extremely variable relative abundances (0.01 to 99%) depending on the species and sample. A core microbiota shared by 70% of the samples indicated a coexistence of lactic acid bacteria with a remarkable level of Streptococcus thermophilus and possible spoilage-associated bacteria, including Pseudomonas, Acinetobacter, and Psychrobacter, with a relative abundance above 50%. The most abundant yeasts were Kluyveromyces marxianus, Yamadazyma triangularis, Trichosporon faecale, and Debaryomyces hansenii. Beta-diversity analyses showed a clear separation of environmental and cheese samples based on both yeast and bacterial community structure. In addition, predicted metagenomes also indicated differential distribution of metabolic pathways between the two categories of samples. Cooccurrence and coexclusion pattern analyses indicated that the occurrence of potential spoilers was excluded by lactic acid bacteria. In addition, their persistence in the environment can be helpful to counter the development of potential spoilers that may contaminate the cheeses, with possible negative effects on their microbiological quality. PMID:26341209

Aerial Vehicles to Detect Maximum Volume of Plume Material Associated with Habitable Areas in Extreme Environments

NASA Technical Reports Server (NTRS)

Gunasekara, Onalli; Wong, Uland Y.; Furlong, Michael P.; Dille, Michael

2017-01-01

Current technologies of exploring habitable areas of icy moons are limited to flybys of space probes. This research project addresses long-term navigation of icy moons by developing a MATLAB adjustable trajectory based on the volume of plume material observed. Plumes expose materials from the sub-surface without accessing the subsurface. Aerial vehicles capable of scouting vapor plumes and detecting maximum plume material volumes, which are considered potentially habitable in inhospitable environments, would enable future deep-space missions to search for extraterrestrial organisms on the surface of icy moons. Although this platform is still a prototype, it demonstrates the potential aerial vehicles can have in improving the capabilities of long-term space navigation and enabling technology for detecting life in extreme environments. Additionally, this work is developing the capabilities that could be utilized as a platform for space biology research. For example, aerial vehicles that are sent to map extreme environments of icy moons or the planet Mars, could also carry small payloads with automated cell-biology experiments, designed to probe the biological response of low-gravity and high-radiation planetary environments, serving as a pathfinder for future human missions.

Copahue Geothermal System: A Volcanic Environment with Rich Extreme Prokaryotic Biodiversity

PubMed Central

Urbieta, María Sofía; Willis Porati, Graciana; Segretín, Ana Belén; González-Toril, Elena; Giaveno, María Alejandra; Donati, Edgardo Rubén

2015-01-01

The Copahue geothermal system is a natural extreme environment located at the northern end of the Cordillera de los Andes in Neuquén province in Argentina. The geochemistry and consequently the biodiversity of the area are dominated by the activity of the Copahue volcano. The main characteristic of Copahue is the extreme acidity of its aquatic environments; ponds and hot springs of moderate and high temperature as well as Río Agrio. In spite of being an apparently hostile location, the prokaryotic biodiversity detected by molecular ecology techniques as well as cultivation shows a rich and diverse environment dominated by acidophilic, sulphur oxidising bacteria or archaea, depending on the conditions of the particular niche studied. In microbial biofilms, found in the borders of the ponds where thermal activity is less intense, the species found are completely different, with a high presence of cyanobacteria and other photosynthetic species. Our results, collected during more than 10 years of work in Copahue, have enabled us to outline geomicrobiological models for the different environments found in the ponds and Río Agrio. Besides, Copahue seems to be the habitat of novel, not yet characterised autochthonous species, especially in the domain Archaea. PMID:27682093

Copahue Geothermal System: A Volcanic Environment with Rich Extreme Prokaryotic Biodiversity.

PubMed

Urbieta, María Sofía; Porati, Graciana Willis; Segretín, Ana Belén; González-Toril, Elena; Giaveno, María Alejandra; Donati, Edgardo Rubén

2015-07-08

The Copahue geothermal system is a natural extreme environment located at the northern end of the Cordillera de los Andes in Neuquén province in Argentina. The geochemistry and consequently the biodiversity of the area are dominated by the activity of the Copahue volcano. The main characteristic of Copahue is the extreme acidity of its aquatic environments; ponds and hot springs of moderate and high temperature as well as Río Agrio. In spite of being an apparently hostile location, the prokaryotic biodiversity detected by molecular ecology techniques as well as cultivation shows a rich and diverse environment dominated by acidophilic, sulphur oxidising bacteria or archaea, depending on the conditions of the particular niche studied. In microbial biofilms, found in the borders of the ponds where thermal activity is less intense, the species found are completely different, with a high presence of cyanobacteria and other photosynthetic species. Our results, collected during more than 10 years of work in Copahue, have enabled us to outline geomicrobiological models for the different environments found in the ponds and Río Agrio. Besides, Copahue seems to be the habitat of novel, not yet characterised autochthonous species, especially in the domain Archaea.

CHOICE - Directed Study: Consequences of longterm- Confinement and Hypobaric HypOxia on Immunity in the Antarctic Concordia Environment

NASA Technical Reports Server (NTRS)

Sams, Clarence; Pierson, Duane; Crucian, Brian; Chouker, Alexander; Feurecker, matthias; Salem, Alexander; Stowe, Raymond; Mehta, Satish; Quiriarte, Heather; Pierson, Duane;

Microfluidic acoustophoretic force based low-concentration oil separation and detection from the environment.

PubMed

Wang, Han; Liu, Zhongzheng; Kim, Sungman; Koo, Chiwan; Cho, Younghak; Jang, Dong-Young; Kim, Yong-Joe; Han, Arum

2014-03-07

Detecting and quantifying extremely low concentrations of oil from the environment have broad applications in oil spill monitoring in ocean and coastal areas as well as in oil leakage monitoring on land. Currently available methods for low-concentration oil detection are bulky or costly with limited sensitivities. Thus they are difficult to be used as portable and field-deployable detectors in the case of oil spills or for monitoring the long-term effects of dispersed oil on marine and coastal ecosystems. Here, we present a low-concentration oil droplet trapping and detection microfluidic system based on the acoustophoresis phenomenon where oil droplets in water having a negative acoustic contrast factor move towards acoustic pressure anti-nodes. By trapping oil droplets from water samples flowing through a microfluidic channel, even very low concentrations of oil droplets can be concentrated to a detectable level for further analyses, which is a significant improvement over currently available oil detection systems. Oil droplets in water were successfully trapped and accumulated in a circular acoustophoretic trapping chamber of the microfluidic device and detected using a custom-built compact fluorescent detector based on the natural fluorescence of the trapped crude oil droplets. After the on-line detection, crude oil droplets released from the trapping chamber were successfully separated into a collection outlet by acoustophoretic force for further off-chip analyses. The developed microfluidic system provides a new way of trapping, detecting, and separating low-concentration crude oil from environmental water samples and holds promise as a low-cost field-deployable oil detector with extremely high sensitivity. The microfluidic system and operation principle are expected to be utilized in a wide range of applications where separating, concentrating, and detecting small particles having a negative acoustic contrast factor are required.

Risk assessment of trace metals in an extreme environment sediment: shallow, hypersaline, alkaline, and industrial Lake Acıgöl, Denizli, Turkey.

PubMed

Budakoglu, Murat; Karaman, Muhittin; Kumral, Mustafa; Zeytuncu, Bihter; Doner, Zeynep; Yildirim, Demet Kiran; Taşdelen, Suat; Bülbül, Ali; Gumus, Lokman

2018-02-23

The major and trace element component of 48 recent sediment samples in three distinct intervals (0-10, 10-20, and 20-30 cm) from Lake Acıgöl is described to present the current contamination levels and grift structure of detrital and evaporate mineral patterns of these sediments in this extreme saline environment. The spatial and vertical concentrations of major oxides were not uniform in the each subsurface interval. However, similar spatial distribution patterns were observed for some major element couples, due mainly to the detrital and evaporate origin of these elements. A sequential extraction procedure including five distinct steps was also performed to determine the different bonds of trace elements in the < 60-μ particulate size of recent sediments. Eleven trace elements (Ni, Fe, Cd, Pb, Cu, Zn, As, Co, Cr, Al and Mn) in nine surface and subsurface sediment samples were analyzed with chemical partitioning procedures to determine the trace element percentage loads in these different sequential extraction phases. The obtained accuracy values via comparison of the bulk trace metal loads with the total loads of five extraction steps were satisfying for the Ni, Fe, Cd, Zn, and Co. While, bulk analysis results of the Cu, Ni, and V elements have good correlation with total organic matter, organic fraction of sequential extraction characterized by Cu, As, Cd, and Pb. Shallow Lake Acıgöl sediment is characteristic with two different redox layer a) oxic upper level sediments, where trace metals are mobilized, b) reduced subsurface level, where the trace metals are precipitated.

Denitrification by extremely halophilic bacteria

NASA Technical Reports Server (NTRS)

Hochstein, L. I.; Tomlinson, G. A.

1985-01-01

Extremely halophilic bacteria were isolated from widely separated sites by anaerobic enrichment in the presence of nitrate. The anaerobic growth of several of these isolates was accompanied by the production of nitrite, nitrous oxide, and dinitrogen. These results are a direct confirmation of the existence of extremely halophilic denitrifying bacteria, and suggest that such bacteria may be common inhabitants of hypersaline environments.

The Nature and Characteristics of Youthful Extremism

ERIC Educational Resources Information Center

Zubok, Iu. A.; Chuprov, V. I.

2010-01-01

Extremism is an acute problem of the present day. Moods of extremism are manifested in all spheres of the life and activities of young people--in education, work, business, political life, and leisure activity. They can be found in both individual and group social self-determination and are influenced by the immediate social environment as well as…

Extremes in ecology: Avoiding the misleading effects of sampling variation in summary analyses

USGS Publications Warehouse

Link, W.A.; Sauer, J.R.

1996-01-01

Surveys such as the North American Breeding Bird Survey (BBS) produce large collections of parameter estimates. One's natural inclination when confronted with lists of parameter estimates is to look for the extreme values: in the BBS, these correspond to the species that appear to have the greatest changes in population size through time. Unfortunately, extreme estimates are liable to correspond to the most poorly estimated parameters. Consequently, the most extreme parameters may not match up with the most extreme parameter estimates. The ranking of parameter values on the basis of their estimates are a difficult statistical problem. We use data from the BBS and simulations to illustrate the potential misleading effects of sampling variation in rankings of parameters. We describe empirical Bayes and constrained empirical Bayes procedures which provide partial solutions to the problem of ranking in the presence of sampling variation.

Aerobic microbial taxa dominate deep subsurface cores from the Alberta oil sands.

PubMed

Ridley, Christina M; Voordouw, Gerrit

2018-06-01

Little is known about the microbial ecology of the subsurface oil sands in Northern Alberta, Canada. Biodegradation of low molecular weight hydrocarbons by indigenous microbes has enriched high molecular weight hydrocarbons, resulting in highly viscous bitumen. This extreme subsurface environment is further characterized by low nutrient availability and limited access to water, thus resulting in low microbial biomass. Improved DNA isolation protocols and increasingly sensitive sequencing methods have allowed an in-depth investigation of the microbial ecology of this unique subsurface environmental niche. Community analysis was performed on core samples (n = 62) that were retrieved from two adjacent sites located in the Athabasca Oil Sands at depths from 220 to 320 m below the surface. Microbial communities were dominated by aerobic taxa, including Pseudomonas and Acinetobacter. Only one core sample microbial community was dominated by anaerobic taxa, including the methanogen Methanoculleus, as well as Desulfomicrobium and Thauera. Although the temperature of the bitumen-containing subsurface is low (8°C), two core samples had high fractions of the potentially thermophilic taxon, Thermus. Predominance of aerobic taxa in the subsurface suggests the potential for in situ aerobic hydrocarbon degradation; however, more studies are required to determine the functional role of these taxa within this unique environment.

Predicting the possibility of not yet observed situations as higher goal of space environment standards.

NASA Astrophysics Data System (ADS)

Nymmik, Rikho

Space environment models are intended for fairly describing the quantitative behavior of nature space environment. Usually, they are constructed on the basis of some experimental data set generalization, which is characteristic of the conditions that were taking place during measurement period. It is often to see that such models state and postulate realities of the past. The typical example of this point of view is the situation around extremely SEP events. During dozens of years models of such events have been based on the largest occurrences observed, which features were measured by some instruments with the reliability that was not always analyzed. It is obvious, that this way does not agree with reality, because any new extreme event conflicts with it. From this follow that space environment models can not be created by using numerical observed data only, when such data are changing in time, or have the probability nature. The model's goal is not only describing the average environment characteristics, but the predicting of extreme ones too. Such a prediction can only be result of analyzing the causes that stimulate environment change and taking them into account in model parameters. In this report we present the analysis of radiation environment formed by solar-generated high energy particles. A progresses and failures of SEP event modeling attempts are also shown and analyzed.

Archaea in artificial environments: Their presence in global spacecraft clean rooms and impact on planetary protection

PubMed Central

Moissl-Eichinger, Christine

2011-01-01

The presence and role of Archaea in artificial, human-controlled environments is still unclear. The search for Archaea has been focused on natural biotopes where they have been found in overwhelming numbers, and with amazing properties. However, they are considered as one of the major group of microorganisms that might be able to survive a space flight, or even to thrive on other planets. Although still concentrating on aerobic, bacterial spores as a proxy for spacecraft cleanliness, space agencies are beginning to consider Archaea as a possible contamination source that could affect future searches for life on other planets. This study reports on the discovery of archaeal 16S rRNA gene signatures not only in US American spacecraft assembly clean rooms but also in facilities in Europe and South America. Molecular methods revealed the presence of Crenarchaeota in all clean rooms sampled, while signatures derived from methanogens and a halophile appeared only sporadically. Although no Archaeon was successfully enriched in our multiassay cultivation approach thus far, samples from a European clean room revealed positive archaeal fluorescence in situ hybridization (FISH) signals of rod-shaped microorganisms, representing the first visualization of Archaea in clean room environments. The molecular and visual detection of Archaea was supported by the first quantitative PCR studies of clean rooms, estimating the overall quantity of Archaea therein. The significant presence of Archaea in these extreme environments in distinct geographical locations suggests a larger role for these microorganisms not only in natural biotopes, but also in human controlled and rigorously cleaned environments. PMID:20703318

Archaea in artificial environments: their presence in global spacecraft clean rooms and impact on planetary protection.

PubMed

Moissl-Eichinger, Christine

2011-02-01

The presence and role of Archaea in artificial, human-controlled environments is still unclear. The search for Archaea has been focused on natural biotopes where they have been found in overwhelming numbers, and with amazing properties. However, they are considered as one of the major group of microorganisms that might be able to survive a space flight, or even to thrive on other planets. Although still concentrating on aerobic, bacterial spores as a proxy for spacecraft cleanliness, space agencies are beginning to consider Archaea as a possible contamination source that could affect future searches for life on other planets. This study reports on the discovery of archaeal 16S rRNA gene signatures not only in US American spacecraft assembly clean rooms but also in facilities in Europe and South America. Molecular methods revealed the presence of Crenarchaeota in all clean rooms sampled, while signatures derived from methanogens and a halophile appeared only sporadically. Although no Archaeon was successfully enriched in our multiassay cultivation approach thus far, samples from a European clean room revealed positive archaeal fluorescence in situ hybridization (FISH) signals of rod-shaped microorganisms, representing the first visualization of Archaea in clean room environments. The molecular and visual detection of Archaea was supported by the first quantitative PCR studies of clean rooms, estimating the overall quantity of Archaea therein. The significant presence of Archaea in these extreme environments in distinct geographical locations suggests a larger role for these microorganisms not only in natural biotopes, but also in human controlled and rigorously cleaned environments.

Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples

PubMed Central

Lu, Qihong; Yu, Ling; Liang, Zhiwei; Yan, Qingyun; He, Zhili; Luan, Tiangang; Liang, Dawei; Wang, Shanquan

2017-01-01

The massive production and improper disposal of organohalides resulted in worldwide contamination in soil and water. However, their environmental survey based on chromatographic methods was hindered by challenges in testing the extremely wide variety of organohalides. Dehalococcoides as obligate organohalide-respiring bacteria exclusively use organohalides as electron acceptors to support their growth, of which the presence could be coupled with organohalides and, therefore, could be employed as a biomarker of the organohalide pollution. In this study, Dehalococcoides was screened in various samples of bioreactors and subsurface environments, showing the wide distribution of Dehalococcoides in sludge and sediment. Further laboratory cultivation confirmed the dechlorination activities of those Dehalococcoides. Among those samples, Dehalococcoides accounting for 1.8% of the total microbial community was found in an anaerobic granular sludge sample collected from a full-scale bioreactor treating petroleum wastewater. Experimental evidence suggested that the influent wastewater in the bioreactor contained bromomethane which support the growth of Dehalococcoides. This study demonstrated that Dehalococcoides could be employed as a promising biomarker to test the present of organohalides in wastestreams or other environmental samples. PMID:28919889

Consistent etiology of severe, frequent psychotic experiences and milder, less frequent manifestations: a twin study of specific psychotic experiences in adolescence.

PubMed

Zavos, Helena M S; Freeman, Daniel; Haworth, Claire M A; McGuire, Philip; Plomin, Robert; Cardno, Alastair G; Ronald, Angelica

2014-09-01

The onset of psychosis is usually preceded by psychotic experiences (PE). Little is known about the etiology of PE and whether the degree of genetic and environmental influences varies across different levels of severity. A recognized challenge is to identify individuals at high risk of developing psychotic disorders prior to disease onset. To investigate the degree of genetic and environmental influences on specific PE, assessed dimensionally, in adolescents in the community and in those who have many, frequent experiences (defined using quantitative cutoffs). We also assessed the degree of overlap in etiological influences between specific PE. Structural equation model-fitting, including univariate and bivariate twin models, liability threshold models, DeFries-Fulker extremes analysis, and the Cherny method, was used to analyze a representative community sample of 5059 adolescent twin pairs (mean [SD] age, 16.31 [0.68] years) from England and Wales. Psychotic experiences assessed as quantitative traits (self-rated paranoia, hallucinations, cognitive disorganization, grandiosity, and anhedonia, as well as parent-rated negative symptoms). Genetic influences were apparent for all PE (15%-59%), with modest shared environment for hallucinations and negative symptoms (17%-24%) and significant nonshared environment (49%-64%) for the self-rated scales and 17% for parent-rated negative symptoms. Three empirical approaches converged to suggest that the etiology in extreme-scoring groups (most extreme scoring: 5%, 10%, and 15%) did not differ significantly from that of the whole distribution. There was no linear change in heritability across the distribution of PE, with the exception of a modest increase in heritability for increasing severity of parent-rated negative symptoms. Of the PE that showed covariation, this appeared to be due to shared genetic influences (bivariate heritabilities, 0.54-0.71). These findings are consistent with the concept of a psychosis continuum, suggesting that the same genetic and environmental factors influence both extreme, frequent PE and milder, less frequent manifestations in adolescents. Individual PE in adolescence, assessed quantitatively, have lower heritability estimates and higher estimates of nonshared environment than those for the liability to schizophrenia. Heritability varies by type of PE, being highest for paranoia and parent-rated negative symptoms and lowest for hallucinations.

Consistent etiology of severe, frequent psychotic experiences and milder, less frequent manifestations: A twin study of specific psychotic experiences in adolescence

PubMed Central

Zavos, Helena M.S.; Freeman, Daniel; Haworth, Claire M. A.; McGuire, Philip; Plomin, Robert; Cardno, Alastair G.; Ronald, Angelica

2014-01-01

Context The onset of psychosis is usually preceded by psychotic experiences, but little is known about their causes. The present study investigated the degree of genetic and environmental influences on specific psychotic experiences, assessed dimensionally, in adolescence in the community and in individuals with many, frequent experiences (defined using quantitative cut-offs). The degree of overlap in etiological influences between specific psychotic experiences was also investigated Objective Investigate degree of genetic and environmental influences on specific psychotic experiences, assessed dimensionally, in adolescence in the community and in individuals having many, frequent experiences (defined using quantitative cut-offs). Test degree of overlap in etiological influences between specific psychotic experiences. Design Classic twin design. Structural equation model-fitting. Univariate and bivariate twin models, liability threshold models, DeFries-Fulker extremes analysis and the Cherny Method. Setting Representative community sample of twins from England and Wales. Participants 5059 adolescent twin pairs (Mean age: 16.31 yrs, SD: 0.68 yrs). Main outcome measure Psychotic experiences assessed as quantitative traits (self-rated paranoia, hallucinations, cognitive disorganization, grandiosity, anhedonia; parent-rated negative symptoms). Results Genetic influences were apparent for all psychotic experiences (15-59%) with modest shared environment for hallucinations and negative symptoms (17-24%) and significant nonshared environment (49-64% for the self-rated scales, 17% for Parent-rated Negative Symptoms). Three different empirical approaches converged to suggest that the etiology in extreme groups (most extreme-scoring 5%, 10% and 15%) did not differ significantly from that of the whole distribution. There was no linear change in the heritability across the distribution of psychotic experiences, with the exception of a modest increase in heritability for increasing severity of parent-rated negative symptoms. Of the psychotic experiences that showed covariation, this appeared to be due to shared genetic influences (bivariate heritabilities = .54-.71). Conclusions and Relevance These findings are consistent with the concept of a psychosis continuum, suggesting that the same genetic and environmental factors influence both extreme, frequent psychotic experiences and milder, less frequent manifestations in adolescents. Individual psychotic experiences in adolescence, assessed quantitatively, have lower heritability estimates and higher estimates of nonshared environment than those for the liability to schizophrenia. Heritability varies by type of psychotic experience, being highest for paranoia and parent-rated negative symptoms, and lowest for hallucinations. PMID:25075799

Extremophiles and the search for extraterrestrial life.

PubMed

Cavicchioli, Ricardo

2002-01-01

Extremophiles thrive in ice, boiling water, acid, the water core of nuclear reactors, salt crystals, and toxic waste and in a range of other extreme habitats that were previously thought to be inhospitable for life. Extremophiles include representatives of all three domains (Bacteria, Archaea, and Eucarya); however, the majority are microorganisms, and a high proportion of these are Archaea. Knowledge of extremophile habitats is expanding the number and types of extraterrestrial locations that may be targeted for exploration. In addition, contemporary biological studies are being fueled by the increasing availability of genome sequences and associated functional studies of extremophiles. This is leading to the identification of new biomarkers, an accurate assessment of cellular evolution, insight into the ability of microorganisms to survive in meteorites and during periods of global extinction, and knowledge of how to process and examine environmental samples to detect viable life forms. This paper evaluates extremophiles and extreme environments in the context of astrobiology and the search for extraterrestrial life.

Characterizing Space Environments with Long-Term Space Plasma Archive Resources

NASA Technical Reports Server (NTRS)

Minow, Joseph I.; Miller, J. Scott; Diekmann, Anne M.; Parker, Linda N.

2009-01-01

A significant scientific benefit of establishing and maintaining long-term space plasma data archives is the ready access the archives afford to resources required for characterizing spacecraft design environments. Space systems must be capable of operating in the mean environments driven by climatology as well as the extremes that occur during individual space weather events. Long- term time series are necessary to obtain quantitative information on environment variability and extremes that characterize the mean and worst case environments that may be encountered during a mission. In addition, analysis of large data sets are important to scientific studies of flux limiting processes that provide a basis for establishing upper limits to environment specifications used in radiation or charging analyses. We present applications using data from existing archives and highlight their contributions to space environment models developed at Marshall Space Flight Center including the Chandra Radiation Model, ionospheric plasma variability models, and plasma models of the L2 space environment.

Proton Tolerance of SiGe Precision Voltage References for Extreme Temperature Range Electronics

NASA Astrophysics Data System (ADS)

Najafizadeh, Laleh; Bellini, Marco; Prakash, A. P. Gnana; Espinel, Gustavo A.; Cressler, John D.; Marshall, Paul W.; Marshall, Cheryl J.

2006-12-01

A comprehensive investigation of the effects of proton irradiation on the performance of SiGe BiCMOS precision voltage references intended for extreme environment operational conditions is presented. The voltage reference circuits were designed in two distinct SiGe BiCMOS technology platforms (first generation (50 GHz) and third generation (200 GHz)) in order to investigate the effect of technology scaling. The circuits were irradiated at both room temperature and at 77 K. Measurement results from the experiments indicate that the proton-induced changes in the SiGe bandgap references are minor, even down to cryogenic temperatures, clearly good news for the potential application of SiGe mixed-signal circuits in emerging extreme environments

Summary of presentation for research on social structure, agreement, and conflict in groups in extreme and isolated environments

NASA Technical Reports Server (NTRS)

1990-01-01

Despite a vast amount of research, little is known concerning the effect of group structure, and individuals' understanding of that structure, on conflict in Antarctic groups. The overall objective of the research discussed is to determine the interrelationships of group structure, social cognition, and group function and conflict in isolated and extreme environments. In the two decades following WWII, a large body of research focused on the physiological, psychological, and social psychological factors affecting the functioning of individuals and groups in a variety of extreme and isolated environments in both the Arctic and Antarctic. There are two primary reasons for further research of this type. First, Antarctic polar stations are considered to be natural laboratories for the social and behavioral sciences and provide an opportunity to address certain theoretical and empirical questions concerned with agreement and conflict in social groups in general and group behavior in extreme, isolated environments in particular. Recent advances in the analysis of social networks and intracultural variation have improved the methods and have shifted the theoretical questions. The research is motivated by three classes of questions: (1) What are the characteristics of the social relations among individuals working and living together in extreme and isolated environments?; (2) What do individuals understand about their group, how does that understanding develop, and how is it socially distributed?; and (3) What is the relationship between that understanding and the functioning of the social group? Answers to these questions are important if we are to advance our knowledge of how individuals and groups adapt to extreme environments. Second, although Antarctic winter-over candidates may be evaluated as qualified on the basis of individual characteristics, they may fail to adapt because of certain characteristics of the social group. Consequently, the ability of winter-over-groups to adapt to these extreme conditions has varied dramatically from year to year. In the past, differences in personality, background, and social status have led to conflicts between individuals or cliques precipitating, in turn, an overall decline in morale, failure to accomplish work tasks, and increases in insomnia, depression, anxiety, and alcohol abuse. A better understanding of the role of group structure and social cognition in processes of group adaptation and conflict in Antarctica would contribute towards the revision of existing screening methods, potentially leading to a reduction of group conflict and improved performance of scientific research and support activities. An improved screening protocol for the Antarctic would also have applications for other isolated environments such as scientific outposts and the proposed NASA space station. In sum, this research will (1) contribute significantly to our theoretical understanding of the role of social structure and cognition in the functioning of groups in isolation; (2) complement current work on health and adaptation in polar environments; and (3) provide for models of the formation of group structure that will aid in the development of improved procedures for assembling groups for the Antarctic and other isolated environments (e.g., space stations).

Preservation of Lipid Biomarkers Under Prolonged and Extreme Hyperaridity in Atacama Desert Soils

NASA Technical Reports Server (NTRS)

Wilhelm, Mary Beth

2015-01-01

Molecular biomarkers are the most direct biosignatures of life on early Earth and a key target in the search for life on Mars. Lipid biomarkers are of particular interest given their ability to survive oxidative degradation and record microbial presence and activity of microorganisms that occurred billions of years ago (Eigenbrode, 2008). Environmental conditions that suspend biotic and abiotic degradative processes prior to lithification can lead to enhanced biomolecular preservation over geological time-scales. The hyperarid core of the Atacama Desert in northern Chile offers a unique environment to investigate lipid biomarker taphonomy under extreme and prolonged dryness. We investigated the accumulation and degree of preservation of lipid biomarkers in million-year-old hyperarid soils where primarily abiotic conditions influence their taphonomy. Soils were extracted and free and membrane bound lipids were analyzed across a vertical profile of 2.5 meters in the Yungay hyper-arid core of the Atacama Desert. Due to the extremely low inventory of biomass in Atacama soils, samples were collected by scientists wearing cleanroom suits to minimize anthropogenic contamination during sampling. Fatty acids were found to be well preserved in Yungay soils, and were most abundant in the clay-rich soils at approx.2 m depth (approx.750 ng of fatty acid methyl ester/g of soil). These buried clays layers were fluvially deposited approximately 2 million years ago, and have been excluded from exposure to rainwater and modern surficial processes since their emplacement (Ewing et al., 2008). Monocarboxylic fatty acid, monohydroxy fatty acid, glycerol tetraether, and n-alkane hydrocarbon content was found to change with depth. Lipid biomarker content in deeper soil layers is suggestive of soils having been formed at a time when environmental conditions were capable of supporting active microbial communities and plants. In short, total lipid extracts reveal a remarkable degree of lipid biomarker preservation even in the oldest soils analyzed (ca. 2 Myr) indicating that typical diagenetic processes of lipid destruction are arrested under extreme dryness. This result has implications for the search for molecular biomarkers on Mars, which could have experienced millions to billions of years of extreme hyperaridity.

Preservation of Lipid Biomarkers Under Prolonged and Extreme Hyperaridity in Atacama Desert Soils

NASA Astrophysics Data System (ADS)

Wilhelm, M. B.; Davila, A. F.; Eigenbrode, J. L.; Parenteau, M. N.; Jahnke, L. L.; Summons, R. E.; Liu, X.; Wray, J. J.; Stamos, B.; O'Reilly, S. S.; Williams, A. J.

2015-12-01

Molecular biomarkers are the most direct biosignatures of life on early Earth and a key target in the search for life on Mars. Lipid biomarkers are of particular interest given their ability to survive oxidative degradation and record microbial presence and activity of microorganisms that occurred billions of years ago (Eigenbrode, 2008). Environmental conditions that suspend biotic and abiotic degradative processes prior to lithification can lead to enhanced biomolecular preservation over geological time-scales. The hyperarid core of the Atacama Desert in northern Chile offers a unique environment to investigate lipid biomarker taphonomy under extreme and prolonged dryness. We investigated the accumulation and degree of preservation of lipid biomarkers in million-year-old hyperarid soils where primarily abiotic conditions influence their taphonomy. Soils were extracted and free and membrane bound lipids were analyzed across a vertical profile of 2.5 meters in the Yungay hyper-arid core of the Atacama Desert. Due to the extremely low inventory of biomass in Atacama soils, samples were collected by scientists wearing cleanroom suits to minimize anthropogenic contamination during sampling. Fatty acids were found to be well preserved in Yungay soils, and were most abundant in the clay-rich soils at ~2 m depth (~750 ng of fatty acid methyl ester/g of soil). These buried clays layers were fluvially deposited approximately 2 million years ago, and have been excluded from exposure to rainwater and modern surficial processes since their emplacement (Ewing et al., 2008). Monocarboxylic fatty acid, monohydroxy fatty acid, glycerol tetraether, and n-alkane hydrocarbon content was found to change with depth. Lipid biomarker content in deeper soil layers is suggestive of soils having been formed at a time when environmental conditions were capable of supporting active microbial communities and plants. In short, total lipid extracts reveal a remarkable degree of lipid biomarker preservation even in the oldest soils analyzed (ca. 2 Myr) indicating that typical diagenetic processes of lipid destruction are arrested under extreme dryness. This result has implications for the search for molecular biomarkers on Mars, which could have experienced millions to billions of years of extreme hyperaridity.

Diversity of bacteria producing pigmented colonies in aerosol, snow and soil samples from remote glacial areas (Antarctica, Alps and Andes)

NASA Astrophysics Data System (ADS)

González-Toril, E.; Amils, R.; Delmas, R. J.; Petit, J.-R.; Komárek, J.; Elster, J.

2008-04-01

Four different communities and one culture of pigmented microbial assemblages were obtained by incubation in mineral medium of samples collected from high elevation snow in the Alps (Mt. Blanc area) and the Andes (Nevado Illimani summit, Bolivia), from Antarctic aerosol (French station Dumont d'Urville) and a maritime Antarctic soil (King George Island, South Shetlands, Uruguay Station Artigas). Molecular analysis of more than 200 16S rRNA gene sequences showed that all cultured cells belong to the Bacteria domain. The phylogenetic comparison with the currently available rDNA database allowed the identification of sequences belonging to Proteobacteria (Alpha-, Beta- and Gamma-proteobacteria), Actinobacteria and Bacteroidetes phyla. The Andes snow culture was the richest in bacterial diversity (eight microorganisms identified) and the maritime Antarctic soil the poorest (only one). Snow samples from Col du midi (Alps) and the Andes shared the highest number of identified microorganisms (Agrobacterium, Limnobacter, Aquiflexus and two uncultured Alphaproteobacteria clones). These two sampling sites also shared four sequences with the Antarctic aerosol sample (Limnobacter, Pseudonocardia and an uncultured Alphaproteobacteria clone). The only microorganism identified in the maritime Antarctica soil (Brevundimonas sp.) was also detected in the Antarctic aerosol. The two snow samples from the Alps only shared one common microorganism. Most of the identified microorganisms have been detected previously in cold environments (Dietzia kujamenisi, Pseudonocardia Antarctica, Hydrogenophaga palleronii and Brebundimonas sp.), marine sediments (Aquiflexus balticus, Pseudomonas pseudoalkaligenes, Pseudomonas sp. and one uncultured Alphaproteobacteria), and soils and rocks (Pseudonocardia sp., Agrobactrium sp., Limnobacter sp. and two uncultured Alphaproteobacetria clones). Air current dispersal is the best model to explain the presence of very specific microorganisms, like those used in this work, in very distant environments. In addition these microorganisms have to be resistant to extreme conditions and able to grow in oligotrophic environments. Considering the habitats in which they have been identified, the presence of pigments must be related with their ability to resist high doses of radiation.

Spawning activity of the Australian lungfish Neoceratodus forsteri in an impoundment.

PubMed

Roberts, D T; Mallett, S; Krück, N C; Loh, W; Tibbetts, I

2014-01-01

This study assessed the spawning activity of the threatened Australian lungfish Neoceratodus forsteri by measuring egg densities within the artificial habitat of a large impoundment (Lake Wivenhoe, Australia). Eggs were sampled (August to November 2009) from multiple locations across the impoundment, but occurred at highest densities in water shallower than 40 cm along shorelines with a dense cover of submerged terrestrial vegetation. The numbers of eggs declined over the study period and all samples were dominated by early developmental stages and high proportions of unviable eggs. The quality of the littoral spawning habitats declined over the study as flooded terrestrial grasses decomposed and filamentous algae coverage increased. Water temperatures at the spawning site exhibited extreme variations, ranging over 20·4° C in water shallower than 5 cm. Dissolved oxygen concentrations regularly declined to <1 mg l⁻¹ at 40 and 80 cm water depth. Spawning habitats utilised by N. forsteri within impoundments expose embryos to increased risk of desiccation or excessive submergence through water-level variations, and extremes in temperature and dissolved oxygen concentration that present numerous challenges for successful spawning and recruitment of N. forsteri in large impoundment environments. © 2014 The Fisheries Society of the British Isles.

Endurance cycling results in extreme environments

NASA Technical Reports Server (NTRS)

Guertin, S. M.; Nguyen, D. N.; Scheick, L. Z.

2003-01-01

A new test bed for life testing flash memories in extreme environments is introducted. the test bed is based on a state-of-the-art development board. Since space applications often desire state-of-the-art devices, such a basis seems appropriate. Comparison of this tester to other such systems, including those with data presented here in the past is made. Limitations of different testers for varying applications are discussed. Recently developed data, using this test bed is also presented.

Manipulating the fidelity of lower extremity visual feedback to identify obstacle negotiation strategies in immersive virtual reality.

PubMed

Kim, Aram; Zhou, Zixuan; Kretch, Kari S; Finley, James M

2017-07-01

The ability to successfully navigate obstacles in our environment requires integration of visual information about the environment with estimates of our body's state. Previous studies have used partial occlusion of the visual field to explore how information about the body and impending obstacles are integrated to mediate a successful clearance strategy. However, because these manipulations often remove information about both the body and obstacle, it remains to be seen how information about the lower extremities alone is utilized during obstacle crossing. Here, we used an immersive virtual reality (VR) interface to explore how visual feedback of the lower extremities influences obstacle crossing performance. Participants wore a head-mounted display while walking on treadmill and were instructed to step over obstacles in a virtual corridor in four different feedback trials. The trials involved: (1) No visual feedback of the lower extremities, (2) an endpoint-only model, (3) a link-segment model, and (4) a volumetric multi-segment model. We found that the volumetric model improved success rate, placed their trailing foot before crossing and leading foot after crossing more consistently, and placed their leading foot closer to the obstacle after crossing compared to no model. This knowledge is critical for the design of obstacle negotiation tasks in immersive virtual environments as it may provide information about the fidelity necessary to reproduce ecologically valid practice environments.

Limits to the thermal tolerance of corals adapted to a highly fluctuating, naturally extreme temperature environment

PubMed Central

Schoepf, Verena; Stat, Michael; Falter, James L.; McCulloch, Malcolm T.

2015-01-01

Naturally extreme temperature environments can provide important insights into the processes underlying coral thermal tolerance. We determined the bleaching resistance of Acropora aspera and Dipsastraea sp. from both intertidal and subtidal environments of the naturally extreme Kimberley region in northwest Australia. Here tides of up to 10 m can cause aerial exposure of corals and temperatures as high as 37 °C that fluctuate daily by up to 7 °C. Control corals were maintained at ambient nearshore temperatures which varied diurnally by 4-5 °C, while treatment corals were exposed to similar diurnal variations and heat stress corresponding to ~20 degree heating days. All corals hosted Symbiodinium clade C independent of treatment or origin. Detailed physiological measurements showed that these corals were nevertheless highly sensitive to daily average temperatures exceeding their maximum monthly mean of ~31 °C by 1 °C for only a few days. Generally, Acropora was much more susceptible to bleaching than Dipsastraea and experienced up to 75% mortality, whereas all Dipsastraea survived. Furthermore, subtidal corals, which originated from a more thermally stable environment compared to intertidal corals, were more susceptible to bleaching. This demonstrates that while highly fluctuating temperatures enhance coral resilience to thermal stress, they do not provide immunity to extreme heat stress events. PMID:26627576

Synthetic extreme environments: overlooked sources of potential biotechnologically relevant microorganisms.

PubMed

Sibanda, Timothy; Selvarajan, Ramganesh; Tekere, Memory

2017-05-01

Synthetic extreme environments like carwash effluent tanks and drains are potential sources of biotechnologically important microorganisms and molecules which have, however, remained unexplored. Using culture- and molecular-based methods, a total of 17 bacterial isolates belonging to the genera Shewanella, Proteus, Paenibacillus, Enterobacter and Citrobacter, Aeromonas, Pseudomonas and Pantoea were identified. Hydrocarbon utilization and enzyme production screening assays showed that Aeromonas sp. CAC11, Paenibacillus sp. CAC12 and Paenibacillus sp. CAC13 and Citrobacter sp. PCW7 were able to degrade benzanthracene, naphthalene and diesel oil, Paenibacillus sp. CAC12 and Paenibacillus sp. CAC13 could produce cellulase enzyme, while Proteus sp. BPS2, Pseudomonas sp. SAS8 and Proteus sp. CAL3 could produce lipase. GC-MS analysis of bacterial secondary metabolites resulted in identification of 107 different compounds produced by Proteus sp. BPS2, Paenibacillus sp. CAC12, Pseudomonas sp. SAS8, Proteus sp. CAL3 and Paenibacillus sp. CAC13. Most of the compounds identified by both GC-MS and LC-MS have previously been determined to have antibacterial, antifungal and/or anticancer properties. Further, microbial metabolites which have previously been known to be produced only by plants or microorganisms found in natural extreme environments were also identified in this study. This research has revealed the immense bioresource potential of microorganisms inhabiting synthetic extreme environments. © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

Limits to the thermal tolerance of corals adapted to a highly fluctuating, naturally extreme temperature environment.

PubMed

Schoepf, Verena; Stat, Michael; Falter, James L; McCulloch, Malcolm T

2015-12-02

Naturally extreme temperature environments can provide important insights into the processes underlying coral thermal tolerance. We determined the bleaching resistance of Acropora aspera and Dipsastraea sp. from both intertidal and subtidal environments of the naturally extreme Kimberley region in northwest Australia. Here tides of up to 10 m can cause aerial exposure of corals and temperatures as high as 37 °C that fluctuate daily by up to 7 °C. Control corals were maintained at ambient nearshore temperatures which varied diurnally by 4-5 °C, while treatment corals were exposed to similar diurnal variations and heat stress corresponding to ~20 degree heating days. All corals hosted Symbiodinium clade C independent of treatment or origin. Detailed physiological measurements showed that these corals were nevertheless highly sensitive to daily average temperatures exceeding their maximum monthly mean of ~31 °C by 1 °C for only a few days. Generally, Acropora was much more susceptible to bleaching than Dipsastraea and experienced up to 75% mortality, whereas all Dipsastraea survived. Furthermore, subtidal corals, which originated from a more thermally stable environment compared to intertidal corals, were more susceptible to bleaching. This demonstrates that while highly fluctuating temperatures enhance coral resilience to thermal stress, they do not provide immunity to extreme heat stress events.

Phylogenetic relationships and taxonomic position of Chlorella-like isolates from low pH environments (pH < 3.0)

PubMed Central

Huss, Volker AR; Ciniglia, Claudia; Cennamo, Paola; Cozzolino, Salvatore; Pinto, Gabriele; Pollio, Antonino

2002-01-01

Background Little is known about phytoplankton communities inhabiting low pH environments such as volcanic and geothermal sites or acidic waters. Only specialised organisms are able to tolerate such extreme conditions. There is, thus, low species diversity. We have characterised the previously isolated acid tolerant Chlorella-like microalgae Viridiella fridericiana and Chlorella protothecoides var. acidicola by microscopical and biomolecular methods in order to assess their phylogenetic relationships. Results Both isolates belong to the trebouxiophycean lineage of chlorophytes. 18S and ITS1 sequence data clearly confirm that Viridiella fridericiana constitutes a new genus apart from the morphologically similar and likewise acid tolerant microalga Chlorella saccharophila. Chlorella protothecoides var. acidicola on the other hand is not a variety of Chlorella protothecoides but falls within a heterogeneous cluster consisting of Nannochloris, "Chlorella" spec. Yanaqocha, and Koliella, and is most closely related to algae which were also isolated from extreme environments. Conclusions The distribution of acid tolerant strains in the 18S rRNA tree shows that acquisition of acid tolerance was unlikely a monophyletic event in green microalgae. We propose that different strains have independently adapted to extreme environments. Some of them have spread worldwide and were able to colonise other extreme habitats. Considering the problems of successfully isolating acid tolerant strains, acidic soils could represent an unsuspected source of biological diversity with high potential for biotechnological utilisations. PMID:12194702

ARC-2008-ACD08-0216-006

NASA Image and Video Library

2008-09-23

Tech Talk on Extreme Rovers: Unveiling the latest findings of Robotic Exploration of Extreme Environments shown in the Immersve Theater NASA Ames Exploration Center Bldg 943A KbalidAl-Ali CMU - West gives presentation on 'Practical Rover Technology'

ARC-2008-ACD08-0216-008

NASA Image and Video Library

2008-09-23

Tech Talk on Extreme Rovers: Unveiling the latest findings of Robotic Exploration of Extreme Environments shown in the Immersve Theater NASA Ames Exploration Center Bldg 943A KbalidAl-Ali CMU - West gives presentation on 'Practical Rover Technology'

ARC-2008-ACD08-0216-007

NASA Image and Video Library

2008-09-23

Tech Talk on Extreme Rovers: Unveiling the latest findings of Robotic Exploration of Extreme Environments shown in the Immersve Theater NASA Ames Exploration Center Bldg 943A KbalidAl-Ali CMU - West gives presentation on 'Practical Rover Technology'

Spatial variability of harmful algal blooms in Milford Lake, Kansas, July and August 2015

USGS Publications Warehouse

Foster, Guy M.; Graham, Jennifer L.; Stiles, Tom C.; Boyer, Marvin G.; King, Lindsey R.; Loftin, Keith A.

2017-01-09

Cyanobacterial harmful algal blooms (CyanoHABs) tend to be spatially variable vertically in the water column and horizontally across the lake surface because of in-lake and weather-driven processes and can vary by orders of magnitude in concentration across relatively short distances (meters or less). Extreme spatial variability in cyanobacteria and associated compounds poses unique challenges to collecting representative samples for scientific study and public-health protection. The objective of this study was to assess the spatial variability of cyanobacteria and microcystin in Milford Lake, Kansas, using data collected on July 27 and August 31, 2015. Spatially dense near-surface data were collected by the U.S. Geological Survey, nearshore data were collected by the Kansas Department of Health and Environment, and open-water data were collected by U.S. Army Corps of Engineers. CyanoHABs are known to be spatially variable, but that variability is rarely quantified. A better understanding of the spatial variability of cyanobacteria and microcystin will inform sampling and management strategies for Milford Lake and for other lakes with CyanoHAB issues throughout the Nation.The CyanoHABs in Milford Lake during July and August 2015 displayed the extreme spatial variability characteristic of cyanobacterial blooms. The phytoplankton community was almost exclusively cyanobacteria (greater than 90 percent) during July and August. Cyanobacteria (measured directly by cell counts and indirectly by regression-estimated chlorophyll) and microcystin (measured directly by enzyme-linked immunosorbent assay [ELISA] and indirectly by regression estimates) concentrations varied by orders of magnitude throughout the lake. During July and August 2015, cyanobacteria and microcystin concentrations decreased in the downlake (towards the outlet) direction.Nearshore and open-water surface grabs were collected and analyzed for microcystin as part of this study. Samples were collected in the uplake (Zone C), midlake (Zone B), and downlake (Zone A) parts of the lake. Overall, no consistent pattern was indicated as to which sample location (nearshore or open water) had the highest microcystin concentrations. In July, the maximum microcystin concentration observed in each zone was detected at a nearshore site, and in August, maximum microcystin concentrations in each zone were detected at an open-water site.The Kansas Department of Health and Environment uses two guidance levels (a watch and a warning level) to issue recreational public-health advisories for CyanoHABs in Kansas lakes. The levels are based on concentrations of microcystin and numbers of cyanobacteria. In July and August, discrete water-quality samples were predominantly indicative of warning status in Zone C, watch status in Zone B, and no advisories in Zone A. Regression-estimated microcystin concentrations, which provided more thorough coverage of Milford Lake (n=683–720) than discrete samples (n=21–24), generally indicated the same overall pattern. Regardless of the individual agencies sampling approach, the overall public-health advisory status of each zone in Milford Lake was similar according to the Kansas Department of Health and Environment guidance levels.

[The heart in extreme sports: hyperbaric activity and microgravity].

PubMed

Berrettini, Umberto; Landolfi, Angelo; Patteri, Giovanna

2008-10-01

The study of the cardiovascular and respiratory modifications in extreme environments could be useful for the understanding of the adaptive mechanisms of the body in particular conditions. The knowledge of how different environmental conditions in terms of extreme pressure, temperature and gravity modify the neurovegetative and cardiovascular system could be useful in daily practice for hypobaric and hyperbaric sports.

Draft genome sequence of the extremely acidophilic biomining bacterium Acidithiobacillus thiooxidans ATCC 19377 provides insights into the evolution of the Acidithiobacillus genus.

PubMed

Valdes, Jorge; Ossandon, Francisco; Quatrini, Raquel; Dopson, Mark; Holmes, David S

2011-12-01

Acidithiobacillus thiooxidans is a mesophilic, extremely acidophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and inorganic sulfur compounds. Here we present the draft genome sequence of A. thiooxidans ATCC 19377, which has allowed the identification of genes for survival and colonization of extremely acidic environments.

Improving power and robustness for detecting genetic association with extreme-value sampling design.

PubMed

Chen, Hua Yun; Li, Mingyao

2011-12-01

Extreme-value sampling design that samples subjects with extremely large or small quantitative trait values is commonly used in genetic association studies. Samples in such designs are often treated as "cases" and "controls" and analyzed using logistic regression. Such a case-control analysis ignores the potential dose-response relationship between the quantitative trait and the underlying trait locus and thus may lead to loss of power in detecting genetic association. An alternative approach to analyzing such data is to model the dose-response relationship by a linear regression model. However, parameter estimation from this model can be biased, which may lead to inflated type I errors. We propose a robust and efficient approach that takes into consideration of both the biased sampling design and the potential dose-response relationship. Extensive simulations demonstrate that the proposed method is more powerful than the traditional logistic regression analysis and is more robust than the linear regression analysis. We applied our method to the analysis of a candidate gene association study on high-density lipoprotein cholesterol (HDL-C) which includes study subjects with extremely high or low HDL-C levels. Using our method, we identified several SNPs showing a stronger evidence of association with HDL-C than the traditional case-control logistic regression analysis. Our results suggest that it is important to appropriately model the quantitative traits and to adjust for the biased sampling when dose-response relationship exists in extreme-value sampling designs. © 2011 Wiley Periodicals, Inc.

Ferroplasma and relatives, recently discovered cell wall-lacking archaea making a living in extremely acid, heavy metal-rich environments.

PubMed

Golyshina, Olga V; Timmis, Kenneth N

2005-09-01

For several decades, the bacterium Acidithiobacillus (previously Thiobacillus) has been considered to be the principal acidophilic sulfur- and iron-oxidizing microbe inhabiting acidic environments rich in ores of iron and other heavy metals, responsible for the metal solubilization and leaching from such ores, and has become the paradigm of such microbes. However, during the last few years, new studies of a number of acidic environments, particularly mining waste waters, acidic pools, etc., in diverse geographical locations have revealed the presence of new cell wall-lacking archaea related to the recently described, acidophilic, ferrous-iron oxidizing Ferroplasma acidiphilum. These mesophilic and moderately thermophilic microbes, representing the family Ferroplasmaceae, were numerically significant members of the microbial consortia of the habitats studied, are able to mobilize metals from sulfide ores, e.g. pyrite, arsenopyrite and copper-containing sulfides, and are more acid-resistant than iron and sulfur oxidizing bacteria exhibiting similar eco-physiological properties. Ferroplasma cell membranes contain novel caldarchaetidylglycerol tetraether lipids, which have extremely low proton permeabilities, as a result of the bulky isoprenoid core, and which are probably a major contributor to the extreme acid tolerance of these cell wall-less microbes. Surprisingly, several intracellular enzymes, including an ATP-dependent DNA ligase have pH optima close to that of the external environment rather than of the cytoplasm. Ferroplasma spp. are probably the major players in the biogeochemical cycling of sulfur and sulfide metals in highly acidic environments, and may have considerable potential for biotechnological applications such as biomining and biocatalysis under extreme conditions.

The Behavior of Hydrogen Under Extreme Conditions on Ultrafast Timescales (A "Life at the Frontiers of Energy Research" contest entry from the 2011 Energy Frontier Research Centers (EFRCs) Summit and Forum)

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

"The Behavior of Hydrogen Under Extreme Conditions on Ultrafast Timescales" was submitted by the Center for Energy Frontier Research in Extreme Environments (EFree) to the "Life at the Frontiers of Energy Research" video contest at the 2011 Science for Our Nation's Energy Future: Energy Frontier Research Centers (EFRCs) Summit and Forum. Twenty-six EFRCs created short videos to highlight their mission and their work. EFree is directed by Ho-kwang Mao at the Carnegie Institute of Science in Washington, DC and is a partnership of scientists from thirteen institutions.The Office of Basic Energy Sciences in the U.S. Department of Energy's Office ofmore » Science established the 46 Energy Frontier Research Centers (EFRCs) in 2009. These collaboratively-organized centers conduct fundamental research focused on 'grand challenges' and use-inspired 'basic research needs' recently identified in major strategic planning efforts by the scientific community. The overall purpose is to accelerate scientific progress toward meeting the nation's critical energy challenges. The mission of Energy Frontier Research in Extreme Environments is 'to accelerate the discovery and creation of energy-relevant materials using extreme pressures and temperatures.' Research topics are: catalysis (CO2, water), photocatalysis, solid state lighting, optics, thermelectric, phonons, thermal conductivity, solar electrodes, fuel cells, superconductivity, extreme environment, radiation effects, defects, spin dynamics, CO2 (capture, convert, store), greenhouse gas, hydrogen (fuel, storage), ultrafast physics, novel materials synthesis, and defect tolerant materials.« less

The Shock Response of Space Bears: The Ability of Life to Survive Some of the Most Extreme Environments Known to Man

NASA Astrophysics Data System (ADS)

Painter, Jonathon; Leighs, James; Appleby-Thomas, Gareth; Hazael, Rachael; McMillan, Paul; Kristensen, Reinhardt

2013-06-01

There have been many recent discoveries of life forms living in environments previously thought to be completely uninhabitable. One particularly interesting discovery of this na- ture is the space bear or tardigrade. The name space bear is a colloquialism applied to the tardigrades because of a recent investigation which saw them being exposed to the vacuum of space and intense solar radiation, and surviving. Tardigrades have the ability to dehy- drate themselves, entering a state called cryptobiosis. This state enables them to survive in the vacuum of space. A single stage gas gun has been employed to uniaxially shock load and subsequently recover tardigrades in both regular and cryptobiotic states. Loading histories were calculated via hydrocode modelling. Survival data is presented comparing shocked and control samples for tardigrades both in normal and cryptobiotic states.

Covariant C and O Isotope Trends in Arctic Carbonate Crusts and ALH 84001: Potential Biomarker or Indicator of Cryogenic Formation Environment?

NASA Technical Reports Server (NTRS)

Socki, Richard A.; Niles, Paul B.; Blake, Weston; Leveille, Richard

2009-01-01

This work seeks to use the chemical, isotopic, and mineralogical characteristics of secondary carbonate minerals produced during brief aqueous events to identify the conditions of the aqueous environment in which they formed. Liquid water near the surface of Mars is subject to either rapid freezing and/or evaporation. These processes are also active on Earth, and produce secondary minerals that have complex chemical, mineralogical, and isotopic textures and compositions that can include covariant relationships between Delta C-13 (sub VPDB) and delta O-18 (sub VSMOW). The extremely well studied four billion year old carbonates preserved in martian meteorite ALH 84001 also show covariant delta C-13 and delta O-18 compositions, but these variations are manifested on a micro-scale in a single thin section while the variation observed so far in terrestrial carbonates is seen between different hand samples.

Bridging the integration gap between imaging and information systems: a uniform data concept for content-based image retrieval in computer-aided diagnosis.

PubMed

Welter, Petra; Riesmeier, Jörg; Fischer, Benedikt; Grouls, Christoph; Kuhl, Christiane; Deserno, Thomas M

2011-01-01

It is widely accepted that content-based image retrieval (CBIR) can be extremely useful for computer-aided diagnosis (CAD). However, CBIR has not been established in clinical practice yet. As a widely unattended gap of integration, a unified data concept for CBIR-based CAD results and reporting is lacking. Picture archiving and communication systems and the workflow of radiologists must be considered for successful data integration to be achieved. We suggest that CBIR systems applied to CAD should integrate their results in a picture archiving and communication systems environment such as Digital Imaging and Communications in Medicine (DICOM) structured reporting documents. A sample DICOM structured reporting template adaptable to CBIR and an appropriate integration scheme is presented. The proposed CBIR data concept may foster the promulgation of CBIR systems in clinical environments and, thereby, improve the diagnostic process.

Bridging the integration gap between imaging and information systems: a uniform data concept for content-based image retrieval in computer-aided diagnosis

PubMed Central

Riesmeier, Jörg; Fischer, Benedikt; Grouls, Christoph; Kuhl, Christiane; Deserno (né Lehmann), Thomas M

2011-01-01

It is widely accepted that content-based image retrieval (CBIR) can be extremely useful for computer-aided diagnosis (CAD). However, CBIR has not been established in clinical practice yet. As a widely unattended gap of integration, a unified data concept for CBIR-based CAD results and reporting is lacking. Picture archiving and communication systems and the workflow of radiologists must be considered for successful data integration to be achieved. We suggest that CBIR systems applied to CAD should integrate their results in a picture archiving and communication systems environment such as Digital Imaging and Communications in Medicine (DICOM) structured reporting documents. A sample DICOM structured reporting template adaptable to CBIR and an appropriate integration scheme is presented. The proposed CBIR data concept may foster the promulgation of CBIR systems in clinical environments and, thereby, improve the diagnostic process. PMID:21672913

Adaptive Tunable Laser Spectrometer for Space Applications

NASA Technical Reports Server (NTRS)

Flesch, Gregory; Keymeulen, Didier

2010-01-01

An architecture and process for the rapid prototyping and subsequent development of an adaptive tunable laser absorption spectrometer (TLS) are described. Our digital hardware/firmware/software platform is both reconfigurable at design time as well as autonomously adaptive in real-time for both post-integration and post-launch situations. The design expands the range of viable target environments and enhances tunable laser spectrometer performance in extreme and even unpredictable environments. Through rapid prototyping with a commercial RTOS/FPGA platform, we have implemented a fully operational tunable laser spectrometer (using a highly sensitive second harmonic technique). With this prototype, we have demonstrated autonomous real-time adaptivity in the lab with simulated extreme environments.

International comparison on the management system of end-of-life vehicles

NASA Astrophysics Data System (ADS)

Wang, Jiaxi; Zhu, Anqi; Zhou, Yaodong

2017-04-01

With the rapid development of economy, Chinese vehicle ownership has risen significantly. At the same time, the end-of-life vehicles put a huge pressure to energy and environment, which will finally have an extremely bad effect on sustainable development of economy. The paper takes the United States, the European Union and Japan as samples, compares and analyses these countries’ experiences in law, administration, operation. The key of developed country governance to solve ‘trust’ problem in recycling is its diversity within a complete legal system. At last, the paper gives some suggestions on the issue of Chinese end-of-life vehicles recycling.

Observations on the use of membrane filtration and liquid impingement to collect airborne microorganisms in various atmospheric environments

USGS Publications Warehouse

Griffin, Dale W.; Gonzalez, C.; Teigell, N.; Petrosky, T.; Northup, D.E.; Lyles, M.

2011-01-01

The influence of sample-collection-time on the recovery of culturable airborne microorganisms using a low-flow-rate membrane-filtration unit and a high-flow-rate liquid impinger were investigated. Differences in recoveries were investigated in four different atmospheric environments, one mid-oceanic at an altitude of ~10.0 m, one on a mountain top at an altitude of ~3,000.0 m, one at ~1.0 m altitude in Tallahassee, Florida, and one at ~1.0 m above ground in a subterranean-cave. Regarding use of membrane filtration, a common trend was observed: the shorter the collection period, the higher the recovery of culturable bacteria and fungi. These data also demonstrated that lower culturable counts were common in the more remote mid-oceanic and mountain-top atmospheric environments with bacteria, fungi, and total numbers averaging (by sample time or method categories) <3.0 colony-forming units (CFU) m -3. At the Florida and subterranean sites, the lowest average count noted was 3.5 bacteria CFU m-3, and the highest averaged 140.4 total CFU m-3. When atmospheric temperature allowed use, the high-volume liquid impinger utilized in this study resulted in much higher recoveries, as much as 10?? greater in a number of the categories (bacterial, fungal, and total CFU). Together, these data illustrated that (1) the high-volume liquid impinger is clearly superior to membrane filtration for aeromicrobiology studies if start-up costs are not an issue and temperature permits use; (2) although membrane filtration is more cost friendly and has a 'typically' wider operational range, its limits include loss of cell viability with increased sample time and issues with effectively extracting nucleic acids for community-based analyses; (3) the ability to recover culturable microorganisms is limited in 'extreme' atmospheric environments and thus the use of a 'limited' methodology in these environments must be taken into account; and (4) the atmosphere culls, i.e., everything is not everywhere. ?? 2010 US Government.

Micro-Vibration Measurements on Thermally Loaded Multi-Layer Insulation Samples in Vacuum

NASA Technical Reports Server (NTRS)

Deutsch, Georg; Grillenbeck, Anton

2008-01-01

Some scientific missions require to an extreme extent the absence of any on-board microvibration. Recent projects dedicated to measuring the Earth's gravity field and modeling the geoid with extremely high accuracy are examples. Their missions demand for extremely low micro-vibration environment on orbit for: (1) Not disturbing the measurement of earth gravity effects with the installed gradiometer or (2) Even not damaging the very high sensitive instruments. Based on evidence from ongoing missions multi-layer insulation (MLI) type thermal control blankets have been identified as a structural element of spacecrafts which might deform under temperature variations being caused by varying solar irradiation in orbit. Any such deformation exerts tiny forces which may cause small reactions resulting in micro-vibrations, in particular by exciting the spacecraft eigenmodes. The principle of the test set-up for the micro-vibration test was as follows. A real side wall panel of the spacecraft (size about 0.25 m2) was low-frequency suspended in a thermal vacuum chamber. On the one side of this panel, the MLI samples were fixed by using the standard methods. In front of the MLI, an IR-rig was installed which provided actively controlled IR-radiation power of about 6 kW/m2 in order to heat the MLI surface. The cooling was passive using the shroud temperature at a chamber pressure <1E-5mbar. The resulting micro-vibrations due to MLI motion in the heating and the cooling phase were measured via seismic accelerometers which were rigidly mounted to the panel. Video recording was used to correlate micro-vibration events to any visual MLI motion. Different MLI sample types were subjected to various thermal cycles in a temperature range between -60 C to +80 C. In this paper, the experience on these micro-vibration measurements will be presented and the conclusions for future applications will be discussed

Using Extreme Groups Strategy When Measures Are Not Normally Distributed.

ERIC Educational Resources Information Center

Fowler, Robert L.

1992-01-01

A Monte Carlo simulation explored how to optimize power in the extreme groups strategy when sampling from nonnormal distributions. Results show that the optimum percent for the extreme group selection was approximately the same for all population shapes, except the extremely platykurtic (uniform) distribution. (SLD)

Heatshield for Extreme Entry Environment Technology (HEEET) for Missions to Saturn and Beyond

NASA Technical Reports Server (NTRS)

Ellerby, D.; Blosser, M.; Chinnapongse, R.; Fowler, M.; Gasch, M.; Hamm, K.; Kazemba, C.; Ma, J.; Milos, F.; Nishioka, O.;

Heatshield for Extreme Entry Environment Technology (HEEET) - Enabling Missions Beyond Heritage Carbon Phenolic

NASA Technical Reports Server (NTRS)

Ellerby, D.; Beerman, A.; Blosser, M.; Boghozian, T.; Chavez-Garcia, J.; Chinnapongse, R.; Fowler, M.; Gage, P.; Gasch, M.; Gonzales, G.;

Heatshield for Extreme Entry Environment Technology (HEEET) Enabling Missions Beyond Heritage Carbon Phenolic

NASA Technical Reports Server (NTRS)

Ellerby, D.; Beerman, A.; Blosser, M.; Boghozian, T.; Chavez-Garcia, J.; Chinnapongse, R.; Fowler, M.; Gage, P.; Gasch, M.; Gonzaes, G.;

Heatshield for Extreme Entry Environment Technology (HEEET) Development and Maturation Status for NF Missions

NASA Technical Reports Server (NTRS)

Ellerby, D.; Blosser, M.; Boghozian, T.; Chavez-Garcia, J.; Chinnapongse, R.; Fowler, M.; Gage, P.; Gasch, M.; Gonzales, G.; Hamm, K.;

Heatshield for Extreme Entry Environment Technology (HEEET) Development and Maturation Status

NASA Technical Reports Server (NTRS)

Ellerby, D.; Boghozian, T.; Driver, D.; Chavez-Garcia, J.; Fowler, M.; Gage, P.; Gasch, M.; Gonzales, G.; Kazemba, C.; Kellermann, C.;

The addition of body armor diminishes dynamic postural stability in military soldiers.

PubMed

Sell, Timothy C; Pederson, Jonathan J; Abt, John P; Nagai, Takashi; Deluzio, Jennifer; Wirt, Michael D; McCord, Larry J; Lephart, Scott M

2013-01-01

Poor postural stability has been identified as a risk factor for lower extremity musculoskeletal injury. The additional weight of body armor carried by Soldiers alters static postural stability and may predispose Soldiers to lower extremity musculoskeletal injuries. However, static postural stability tasks poorly replicate the dynamic military environment, which places considerable stress on the postural control system during tactical training and combat. Therefore, the purpose of this study was to examine the effects of body armor on dynamic postural stability during single-leg jump landings. Thirty-six 101st Airborne Division (Air Assault) Soldiers performed single-leg jump landings in the anterior direction with and without wearing body armor. The dynamic postural stability index and the individual stability indices (medial-lateral stability index, anterior-posterior stability index, and vertical stability index) were calculated for each condition. Paired sample t-tests were performed to determine differences between conditions. Significant differences existed for the medial-lateral stability index, anterior-posterior stability index, vertical stability index, and dynamic postural stability index (p < 0.05). The addition of body armor resulted in diminished dynamic postural stability, which may result in increased lower extremity injuries. Training programs should address the altered dynamic postural stability while wearing body armor in attempts to promote adaptations that will result in safer performance during dynamic tasks.

Multifunctional Electroactive Nanocomposites Based on Piezoelectric Boron Nitride Nanotubes.

PubMed

Kang, Jin Ho; Sauti, Godfrey; Park, Cheol; Yamakov, Vesselin I; Wise, Kristopher E; Lowther, Sharon E; Fay, Catharine C; Thibeault, Sheila A; Bryant, Robert G

2015-12-22

Space exploration missions require sensors and devices capable of stable operation in harsh environments such as those that include high thermal fluctuation, atomic oxygen, and high-energy ionizing radiation. However, conventional or state-of-the-art electroactive materials like lead zirconate titanate, poly(vinylidene fluoride), and carbon nanotube (CNT)-doped polyimides have limitations on use in those extreme applications. Theoretical studies have shown that boron nitride nanotubes (BNNTs) have strength-to-weight ratios comparable to those of CNTs, excellent high-temperature stability (to 800 °C in air), large electroactive characteristics, and excellent neutron radiation shielding capability. In this study, we demonstrated the experimental electroactive characteristics of BNNTs in novel multifunctional electroactive nanocomposites. Upon application of an external electric field, the 2 wt % BNNT/polyimide composite was found to exhibit electroactive strain composed of a superposition of linear piezoelectric and nonlinear electrostrictive components. When the BNNTs were aligned by stretching the 2 wt % BNNT/polyimide composite, electroactive characteristics increased by about 460% compared to the nonstretched sample. An all-nanotube actuator consisting of a BNNT buckypaper layer between two single-walled carbon nanotube buckypaper electrode layers was found to have much larger electroactive properties. The additional neutron radiation shielding properties and ultraviolet/visible/near-infrared optical properties of the BNNT composites make them excellent candidates for use in the extreme environments of space missions.

Nutritional status changes in humans during a 14-day saturation dive: the NASA Extreme Environment Mission Operations V project

NASA Technical Reports Server (NTRS)

Smith, Scott M.; Davis-Street, Janis E.; Fesperman, J. Vernell; Smith, Myra D.; Rice, Barbara L.; Zwart, Sara R.

2004-01-01

Ground-based analogs of spaceflight are an important means of studying physiologic and nutritional changes associated with space travel, and the NASA Extreme Environment Mission Operations V (NEEMO) is such an analog. To determine whether saturation diving has nutrition-related effects similar to those of spaceflight, we conducted a clinical nutritional assessment of the NEEMO crew (4 men, 2 women) before, during, and after their 14-d saturation dive. Blood and urine samples were collected before, during, and after the dive. The foods consumed by the crew were typical of the spaceflight food system. A number of physiologic changes were observed, during and after the dive, that are also commonly observed during spaceflight. Hemoglobin and hematocrit were lower (P < 0.05) after the dive. Transferrin receptors were significantly lower immediately after the dive. Serum ferritin increased significantly during the dive. There was also evidence indicating that oxidative damage and stress increased during the dive. Glutathione peroxidase and superoxide dismutase decreased during and after the dive (P < 0.05). Decreased leptin during the dive (P < 0.05) may have been related to the increased stress. Subjects had decreased energy intake and weight loss during the dive, similar to what is observed during spaceflight. Together, these similarities to spaceflight provide a model to use in further defining the physiologic effects of spaceflight and investigating potential countermeasures.

Multifunctional Electroactive Nanocomposites Based on Piezoelectric Boron Nitride Nanotubes

NASA Technical Reports Server (NTRS)

Kang, Jin Ho; Sauti, Godfrey; Park, Cheol; Yamakov, Vesselin I.; Wise, Kristopher E.; Lowther, Sharon E.; Fay, Catharine C.; Thibeault, Sheila A.; Bryant, Robert G.

2015-01-01

Space exploration missions require sensors and devices capable of stable operation in harsh environments such as those that include high thermal fluctuation, atomic oxygen, and high-energy ionizing radiation. However, conventional or state-of-the-art electroactive materials like lead zirconate titanate, poly(vinylidene fluoride), and carbon nanotube (CNT)-doped polyimides have limitations on use in those extreme applications. Theoretical studies have shown that boron nitride nanotubes (BNNTs) have strength-to-weight ratios comparable to those of CNTs, excellent high-temperature stability (to 800 C in air), large electroactive characteristics, and excellent neutron radiation shielding capability. In this study, we demonstrated the experimental electroactive characteristics of BNNTs in novel multifunctional electroactive nanocomposites. Upon application of an external electric field, the 2 wt % BNNT/polyimide composite was found to exhibit electroactive strain composed of a superposition of linear piezoelectric and nonlinear electrostrictive components. When the BNNTs were aligned by stretching the 2 wt % BNNT/polyimide composite, electroactive characteristics increased by about 460% compared to the nonstretched sample. An all-nanotube actuator consisting of a BNNT buckypaper layer between two single-walled carbon nanotube buck-paper electrode layers was found to have much larger electroactive properties. The additional neutron radiation shielding properties and ultraviolet/visible/near-infrared optical properties of the BNNT composites make them excellent candidates for use in the extreme environments of space missions. utilizing the unique characteristics of BNNTs.

Working hours, sleep, salivary cortisol, fatigue and neuro-behavior during Mars analog mission: five crews study.

PubMed

Rai, Balwant; Foing, Bernard H; Kaur, Jasdeep

2012-05-16

The buoyancy of humans in exploring extreme space environments has been established during missions to the moon. Long duration missions like mission to Mars however, requires humans to adapt to systemic and complex environments beyond the human body's capacity. Astronauts will encounter both physiological and psychological extremes during this trip. Very few studies are conducted on effect of long duration work and sleepiness on cognitive performance. So, this study was planned to find out effects of leadership responsibility, sleepiness and long duration working hours on cognitive performance. The 30 members (leadership: normal; 10:20) were selected from MDRS crews (Mars Desert Research Station, USA). Neurobehavioral test performance, self-ratings of fatigue and sleepiness, and salivary cortisol levels were evaluated during first day, mid and end day of mission. The leadership group did not show any signs of reduced test performance, even in elevated fatigue and sleepiness. The leadership group had faster reaction times on end of mission as compared to first and after 7 day of mission. Salivary cortisol levels were significantly higher in leadership group as compared to normal group. The results suggest that long duration work and sleepiness does not affect the cognitive performance of crew member. Further study is required while taking into account all factors and large sample size to prove this fact. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Extreme Magnitude Earthquakes and their Economical Consequences

NASA Astrophysics Data System (ADS)

Chavez, M.; Cabrera, E.; Ashworth, M.; Perea, N.; Emerson, D.; Salazar, A.; Moulinec, C.

2011-12-01

The frequency of occurrence of extreme magnitude earthquakes varies from tens to thousands of years, depending on the considered seismotectonic region of the world. However, the human and economic losses when their hypocenters are located in the neighborhood of heavily populated and/or industrialized regions, can be very large, as recently observed for the 1985 Mw 8.01 Michoacan, Mexico and the 2011 Mw 9 Tohoku, Japan, earthquakes. Herewith, a methodology is proposed in order to estimate the probability of exceedance of: the intensities of extreme magnitude earthquakes, PEI and of their direct economical consequences PEDEC. The PEI are obtained by using supercomputing facilities to generate samples of the 3D propagation of extreme earthquake plausible scenarios, and enlarge those samples by Monte Carlo simulation. The PEDEC are computed by using appropriate vulnerability functions combined with the scenario intensity samples, and Monte Carlo simulation. An example of the application of the methodology due to the potential occurrence of extreme Mw 8.5 subduction earthquakes on Mexico City is presented.

High Temperature Electronics for Intelligent Harsh Environment Sensors

NASA Technical Reports Server (NTRS)

Evans, Laura J.

2008-01-01

The development of intelligent instrumentation systems is of high interest in both public and private sectors. In order to obtain this ideal in extreme environments (i.e., high temperature, extreme vibration, harsh chemical media, and high radiation), both sensors and electronics must be developed concurrently in order that the entire system will survive for extended periods of time. The semiconductor silicon carbide (SiC) has been studied for electronic and sensing applications in extreme environment that is beyond the capability of conventional semiconductors such as silicon. The advantages of SiC over conventional materials include its near inert chemistry, superior thermomechanical properties in harsh environments, and electronic properties that include high breakdown voltage and wide bandgap. An overview of SiC sensors and electronics work ongoing at NASA Glenn Research Center (NASA GRC) will be presented. The main focus will be two technologies currently being investigated: 1) harsh environment SiC pressure transducers and 2) high temperature SiC electronics. Work highlighted will include the design, fabrication, and application of SiC sensors and electronics, with recent advancements in state-of-the-art discussed as well. These combined technologies are studied for the goal of developing advanced capabilities for measurement and control of aeropropulsion systems, as well as enhancing tools for exploration systems.

40 CFR 355.15 - Which threshold planning quantity do I use for an extremely hazardous substance present at my...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 29 2013-07-01 2013-07-01 false Which threshold planning quantity do I use for an extremely hazardous substance present at my facility in solid form? 355.15 Section 355.15... § 355.15 Which threshold planning quantity do I use for an extremely hazardous substance present at my...

Investigating Extreme Lifestyles through Mangrove Transcriptomics

ERIC Educational Resources Information Center

Dassanayake, Maheshi

2009-01-01

Mangroves represent phylogenetically diverse taxa in tropical coastal terrestrial habitats. They are extremophiles, evolutionarily adapted to tolerate flooding, anoxia, high temperatures, wind, and high and extremely variable salt conditions in typically resource-poor environments. The genetic basis for these adaptations is, however, virtually…

Smell Disorders

MedlinePlus

... the smell. There are more smells in the environment than there are receptors, and any given molecule ... sensory nerve cells. Understand the effects of the environment (such as gasoline fumes, chemicals, and extremes of ...

Prediction of human adaptation and performance in underwater environments.

PubMed

Colodro Plaza, Joaquín; Garcés de los Fayos Ruiz, Enrique J; López García, Juan J; Colodro Conde, Lucía

2014-01-01

Environmental stressors require the professional diver to undergo a complex process of psychophysiological adaptation in order to overcome the demands of an extreme environment and carry out effective and efficient work under water. The influence of cognitive and personality traits in predicting underwater performance and adaptation has been a common concern for diving psychology, and definitive conclusions have not been reached. In this ex post facto study, psychological and academic data were analyzed from a large sample of personnel participating in scuba diving courses carried out in the Spanish Navy Diving Center. In order to verify the relevance of individual differences in adaptation to a hostile environment, we evaluated the predictive validity of general mental ability and personality traits with regression techniques. The data indicated the existence of psychological variables that can predict the performance ( R² = .30, p <.001) and adaptation ( R²(N) = .51, p <.001) of divers in underwater environment. These findings support the hypothesis that individual differences are related to the probability of successful adaptation and effective performance in professional diving. These results also verify that dispositional traits play a decisive role in diving training and are significant factors in divers' psychological fitness.

Adaptation to the deep-sea hydrothermal vents and cold seeps: Insights from the transcriptomes of Alvinocaris longirostris in both environments

NASA Astrophysics Data System (ADS)

Hui, Min; Cheng, Jiao; Sha, Zhongli

2018-05-01

Alvinocaris longirostris Kikuchi and Ohta, 1995 is one of the few species co-distributed in deep-sea hydrothermal vent and cold seep environments. We performed the transcriptome analysis for A. longirostris and identified differentially expressed genes (DEGs) between samples from the Iheya North hydrothermal vent (HV) and a methane seep in the South China Sea (MS). From the 57,801 annotated unigenes, multi-copies of enzyme family members for eliminating toxic xenobiotics were isolated and seven putatively duplicated gene clusters of cytochrome P450s were discovered, which may contribute to adaptation to the harsh conditions. Eight single amino acid substitutions of a Rhodopsin gene with low expression in two deep-sea alvinocaridid species were positively selected when compared with shallow water shrimps, which may be the result of adaptation to the dim-light environment in deep sea. 408 DEGs were identified with 53 and 355 up-regulated in HV and MS, respectively. Various genes associated with sulfur metabolism, detoxification and mitochondria were included, revealing different mechanisms of adaptation to the two types of extreme environments. All results are expected to serve as important basis for the further study.

Archaea were widespread in sediments of the Messinian Salinity Crisis

NASA Astrophysics Data System (ADS)

Birgel, Daniel; Peckmann, Jörn

2015-04-01

The Messinian salinity crisis (MSC) was among the most extreme and short-lived paleooceanographic events in Earth history and dramatically impacted the depositional environments of the Mediterranean. Many of the Messinian sedimentary sequences reflect environmental variability on extremely short time scales, typified by phenomena like evaporation and high salinities, anoxia, and desiccation. Only few organisms tolerate such severe conditions. Among those are archaea, many of which are especially well adapted to extreme conditions. We studied various MSC locations and deposits to shed light onto the role of archaea in the MSC, focusing on lipid biomarkers. These are (1) primary gypsum with abundant, yet problematic filamentous microfossils from various locations in the Mediterranean, (2) Calcare di Base, limestones from Sicily and Calabria, and (3) Calcare Solfifero, authigenic carbonates associated with native sulfur from Sicily. (1) Primary gypsum beds with abundant filamentous fossils are widespread in the Mediterranean. Archaea were found as important contributor of organic matter in these evaporites. The filaments, however, have previously been interpreted to represent cyanobacteria based on the extraction and amplification of cyanobacterial DNA. Cyanobacteria produce specific and long-lasting biomarkers, but no such compounds were found in the studied deposits, thus, the assignment of the filaments to cyanobacteria necessitates further verification. (2) The Calcare di Base are widespread, genetically heterogeneous Messinian limestones, which are particularly common in Sicily and Calabria. The environmental conditions during their deposition, as well as mechanisms and timing of formation are a matter of debate. The studied Calcare di Base samples were found to contain specific halophilic archaeal signatures and numerous pseudomorphs after halite. (3) The Calcare Solfifero, authigenic carbonates accompanied by elemental sulfur formed in the course of microbial sulfate reduction. One of the important processes fuelling authigenesis was microbial oxidation of methane. Lipid biomarker patterns reveal that a consortium of methanotrophic archaea and sulfate-reducing bacteria consumed methane in anoxic and hypersaline environments. Halophilic archaea other than those archaea involved in methane oxidation have been present in the depositional environment as well. This as to yet still somewhat random selection of examples provides evidence for the great diversity of environmental settings created during the MSC and the abundance of archaea in these environments, calling for more work on the geomicrobiology of the unrivaled archive of dramatic paleooceanographic change during the MSC.

Thermal stress, human performance, and physical employment standards.

PubMed

Cheung, Stephen S; Lee, Jason K W; Oksa, Juha

2016-06-01

Many physically demanding occupations in both developed and developing economies involve exposure to extreme thermal environments that can affect work capacity and ultimately health. Thermal extremes may be present in either an outdoor or an indoor work environment, and can be due to a combination of the natural or artificial ambient environment, the rate of metabolic heat generation from physical work, processes specific to the workplace (e.g., steel manufacturing), or through the requirement for protective clothing impairing heat dissipation. Together, thermal exposure can elicit acute impairment of work capacity and also chronic effects on health, greatly contributing to worker health risk and reduced productivity. Surprisingly, in most occupations even in developed economies, there are rarely any standards regarding enforced heat or cold safety for workers. Furthermore, specific physical employment standards or accommodations for thermal stressors are rare, with workers commonly tested under near-perfect conditions. This review surveys the major occupational impact of thermal extremes and existing employment standards, proposing guidelines for improvement and areas for future research.

[Vulnerability assessment of eco-environment in Yimeng mountainous area of Shandong Province based on SRP conceptual model].

PubMed

Liu, Zheng-jia; Yu, Xing-xiu; Li, Lei; Huang, Mei

2011-08-01

Based on the ecological sensitivity-resilience-pressure (SRP) conceptual model, and selecting 13 indices including landscape diversity index, soil erosion, and elevation, etc. , the vulnerability of the eco-environment in Yimeng mountainous area of Shandong Province was assessed under the support of GIS and by using principal component analysis and hierarchy analytical method. According to the eco-environmental vulnerability index (EVI) values, the eco-environment vulnerability of study area was classified into 5 levels, i.e., slight (<1.8), light (1.8-2.8), moderate (2.8-3.5), heavy (3.5-4.0), and extreme vulnerability (>4.0). In the study area, moderately vulnerable area occupied 43.3% of the total, while the slightly, lightly, heavily, and extremely vulnerable areas occupied 6.1%, 33.8%, 15.9%, and 0.9%, respectively. The heavily and extremely vulnerable areas mainly located in the topographically complicated hilly area or the hill-plain ecotone with frequent human activities.

A Silicon Carbide Wireless Temperature Sensing System for High Temperature Applications

PubMed Central

Yang, Jie

2013-01-01

In this article, an extreme environment-capable temperature sensing system based on state-of-art silicon carbide (SiC) wireless electronics is presented. In conjunction with a Pt-Pb thermocouple, the SiC wireless sensor suite is operable at 450 °C while under centrifugal load greater than 1,000 g. This SiC wireless temperature sensing system is designed to be non-intrusively embedded inside the gas turbine generators, acquiring the temperature information of critical components such as turbine blades, and wirelessly transmitting the information to the receiver located outside the turbine engine. A prototype system was developed and verified up to 450 °C through high temperature lab testing. The combination of the extreme temperature SiC wireless telemetry technology and integrated harsh environment sensors will allow for condition-based in-situ maintenance of power generators and aircraft turbines in field operation, and can be applied in many other industries requiring extreme environment monitoring and maintenance. PMID:23377189

Evaluation of Potential Climate Change Impacts on Particle Movement in Open Channel Flow

NASA Astrophysics Data System (ADS)

Lin, E.; Tsai, C.

2014-12-01

It is important to develop a forecast model to predict the trajectory of sediment particles when extreme flow events occur. In extreme flow environments, the stochastic jump diffusion particle tracking model (SJD-PTM) can be used to model the movement of sediment particles in response to extreme events. This proposed SJD-PTM can be separated into three main parts — a drift motion, a turbulence term and a jump term due to random occurrences of extreme flow events. The study is intended to modify the jump term, which models the abrupt changes of particle position in the extreme flow environments. The frequency of extreme flow occurrences might change due to many uncertain factors such as climate change. The study attempts to use the concept of the logistic regression and the parameter of odds ratio, namely the trend magnitude to investigate the frequency change of extreme flow event occurrences and its impact on sediment particle movement. With the SJD-PTM, the ensemble mean and variance of particle trajectory can be quantified via simulations. The results show that by taking the effect of the trend magnitude into consideration, the particle position and its uncertainty may undergo a significant increase. Such findings will have many important implications to the environmental and hydraulic engineering design and planning. For instance, when the frequency of the occurrence of flow events with higher extremity increases, particles can travel further and faster downstream. It is observed that flow events with higher extremity can induce a higher degree of entrainment and particle resuspension, and consequently more significant bed and bank erosion.

Effects of service environments on aluminum-brazed titanium (ABTi)

NASA Technical Reports Server (NTRS)

Cotton, W. L.

1978-01-01

Aluminum brazed titanium (ABTi) structures were evaluated during prolonged exposure to extreme environments: elevated temperature exposure to airline service fluids, hydraulic fluid, and seawater, followed by laboratory corrosion tests. Solid-face and perforated face honeycomb sandwich panel specimens, stressed panel assemblies, and faying surface brazed joints were tested. The corrosion resistance of ABTi is satisfactory for commercial airline service. Unprotected ABTi proved inherently resistant to attack by all of the extreme service aircraft environments except: seawater at 700 K (800 F) and above, dripping phosphate ester hydraulic fluid at 505 K (450 F), and a marine environment at ambient temperature. The natural oxides and deposits present on titanium surfaces in airline service provide protection against hot salt corrosion pitting. Coatings are required to protect titanium dripping phosphate ester fluid at elevated temperatures and to protect exposed acoustic honeycomb parts against corrosion in a marine environment.

Stratospheric microbiology at 20 km over the Pacific Ocean

USGS Publications Warehouse

Smith, David J.; Griffin, Dale W.; Schuerger, Andrew C.

2010-01-01

An aerobiology sampling flight at 20 km was conducted on 28 April 2008 over the Pacific Ocean (36.5° N, 118–149° W), a period of time that coincided with the movement of Asian dust across the ocean. The aim of this study was to confirm the presence of viable bacteria and fungi within a transoceanic, atmospheric bridge and to improve the resolution of flight hardware processing techniques. Isolates of the microbial strains recovered were analyzed with ribosomal ribonucleic acid (rRNA) sequencing to identify bacterial species Bacillus sp., Bacillus subtilis, Bacillus endophyticus, and the fungal genus Penicillium. Satellite imagery and ground-based radiosonde observations were used to measure dust movement and characterize the high-altitude environment at the time of collection. Considering the atmospheric residency time (7–10 days), the extreme temperature regime of the environment (-75°C), and the absence of a mechanism that could sustain particulates at high altitude, it is unlikely that our samples indicate a permanent, stratospheric ecosystem. However, the presence of viable fungi and bacteria in transoceanic stratosphere remains relevant to understanding the distribution and extent of microbial life on Earth.

Fetal exposures and perinatal influences on the stool microbiota of premature infants

PubMed Central

Chernikova, Diana A.; Koestler, Devin C.; Hoen, Anne Gatewood; Housman, Molly L.; Hibberd, Patricia L.; Moore, Jason H.; Morrison, Hilary G.; Sogin, Mitchell L.; Ul-Abideen, Muhammad Zain; Madan, Juliette C.

2015-01-01

Objective To test the hypothesis that maternal complications significantly affect gut colonization patterns in very low birth weight infants. Methods 49 serial stool samples were obtained weekly from 9 extremely premature infants enrolled in a prospective longitudinal study. Sequencing of the bacterial 16S rRNA gene from stool samples was performed to approximate the intestinal microbiome. Linear mixed effects models were used to evaluate relationships between perinatal complications and intestinal microbiome development. Results Subjects with prenatal exposure to a non-sterile intrauterine environment, i.e. PPPROM and chorioamnionitis exposure, were found to have a relatively higher abundance of potentially pathogenic bacteria in the stool across all time points compared to subjects without those exposures, irrespective of exposure to postnatal antibiotics. Compared with those delivered by Caesarean section, vaginally delivered subjects were found to have significantly lower diversity of stool microbiota across all time points, with lower abundance of many genera, most in the family Enterobacteriaceae. Conclusions We identified persistently increased potential pathogen abundance in the developing stool microbiota of subjects exposed to a non-sterile uterine environment. Maternal complications appear to significantly influence the diversity and bacterial composition of the stool microbiota of premature infants, with findings persisting over time. PMID:25394613

Development of a flow system for studying biofilm formation on medical devices with microcalorimetry.

PubMed

Said, Jawal; Walker, Michael; Parsons, David; Stapleton, Paul; Beezer, Anthony E; Gaisford, Simon

2015-04-01

Isothermal microcalorimetry (IMC) is particularly suited to the study of microbiological samples in complex or heterogeneous environments because it does not require optical clarity of the sample and can detect metabolic activity from as few as 10(4) CFU/mL cells. While the use of IMC for studying planktonic cultures is well established, in the clinical environment bacteria are most likely to be present as biofilms. Biofilm prevention and eradication present a number of challenges to designers and users of medical devices and implants, since bacteria in biofilm colonies are usually more resistant to antimicrobial agents. Analytical tools that facilitate investigation of biofilm formation are therefore extremely useful. While it is possible to study pre-prepared biofilms in closed ampoules, better correlation with in vivo behaviour can be achieved using a system in which the bacterial suspension is flowing. Here, we discuss the potential of flow microcalorimetry for studying biofilms and report the development of a simple flow system that can be housed in a microcalorimeter. The use of the flow system is demonstrated with biofilms of Staphylococcus aureus. Copyright © 2014 Elsevier Inc. All rights reserved.

Self-Reported Extremely Adverse Life Events and Longitudinal Changes in Five-Factor Model Personality Traits in an Urban Sample

PubMed Central

Löckenhoff, Corinna E.; Terracciano, Antonio; Patriciu, Nicholas S.; Eaton, William W.; Costa, Paul T.

2009-01-01

This study examined longitudinal personality change in response to extremely adverse life events in a sample (N = 458) drawn from the East Baltimore Epidemiologic Catchment Area study. Five-factor model personality traits were assessed twice over an average interval of 8 years. Twenty-five percent of the participants reported an extremely horrifying or frightening event within 2 years before the second personality assessment. Relative to the rest of the sample, they showed increases in neuroticism, decreases in the compliance facet of agreeableness, and decreases in openness to values. Baseline personality was unrelated to future events, but among participants who reported extreme events, lower extraversion and/or conscientiousness at baseline as well as longitudinal increases in neuroticism predicted lower mental health at follow-up. PMID:19230009

Resistance of spacecraft isolates to outer space for planetary protection purposes -first results of the experiment PROTECT of the EXPOSE-E mission.

NASA Astrophysics Data System (ADS)

Horneck, Gerda; Moeller, Ralf

Spore-forming microbes are of particular concern in the context of planetary protection, be-cause their endospores are highly resistant to a variety of environmental extremes, including certain sterilization procedures and the harsh environment of outer space or planetary sur-faces (Nicholson et al., 2000; Horneck et al. 2009). Furthermore, isolates from space craft and space craft assembly facilities have been identified that form spores of an elevated resistance to various physical and chemical conditions, such as ionizing and UV radiation, desiccation and oxidative stress (La Duc et al., 2007). This observation led to the supposition that the spe-cial conditions of ultraclean spacecraft assembly facilities and the applied spacecraft cleaning and decontamination measures cause a selection of the most resistant organisms as survivors. To test this hypothesis, spores of B. pumilus SAFR-032 isolated from these environments as well as spores of the laboratory strain B. subtilis 168 were subjected to selected parameters of space in the experiment PROTECT during the EXPOSE-E mission (February 7, 2008 -September 12, 2009), attached to the EuTEF platform outside of the Columbus module of the International Space Station. The spores were mounted as dry layers onto spacecraft-qualified material (aluminum coupons) and exposed to the following parameters of space, applied sep-arately or in selected combinations: (i) space vacuum, (ii) solar extraterrestrial UV radiation including vacuum-UV, (iii) simulated Mars atmosphere and UV radiation climate, and (iv) galactic cosmic radiation. After recovery, visual inspection showed color changes of the sun-exposed spore samples from white to brownish demonstrating photochemical damage caused by solar extraterrestrial UV radiation. On-going analyses include studies of viability and capabil-ity of repair of damage, mutagenic spectrum, e.g. trp-revertants, rifampicin-resistant mutants, DNA lesion, global gene expression, and genomic and proteomic characterizations using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). First viability studies gave the following survival rates: 20 -30 References: Horneck,G., D.M. Klaus, R.L. Mancinelli (2010) Space microbiology, Microb. Mol. Biol. Rev. (in press) La Duc MT, Dekas A, Osman S, Moissl C, Newcombe D, Venkateswaran K. (2007) Isolation and character-ization of bacteria capable of tolerating the extreme conditions of clean room environments. Appl Environ Microbiol. 73, 2600-11. Nicholson WL, Munakata N, Horneck G, Melosh HJ, and Setlow P (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments, Microb. Mol. Biol. Rev. 64, 548-572.

Characterization of atmospheric emission sources in lichen from metal and organic contaminant patterns.

PubMed

Ratier, Aude; Dron, Julien; Revenko, Gautier; Austruy, Annabelle; Dauphin, Charles-Enzo; Chaspoul, Florence; Wafo, Emmanuel

2018-03-01

Lichen samples from contrasted environments, influenced by various anthropic activities, were investigated focusing on the contaminant signatures according to the atmospheric exposure typologies. Most of the contaminant concentrations measured in the 27 lichen samples, collected around the industrial harbor of Fos-sur-Mer (France), were moderate in rural and urban environments, and reached extreme levels in industrial areas and neighboring cities (Al up to 6567 mg kg -1 , Fe 42,398 mg kg -1 , or ΣPAH 1417 μg kg -1 for example). At the same time, a strong heterogeneity was noticed in industrial samples while urban and rural ones were relatively homogeneous. Several metals could be associated to steel industry (Fe, Mn, Cd), road traffic, and agriculture (Sb, Cu, Sn), or to a distinct chemical installation (Mo). As well, PCDFs dominated in industrial samples while PCDDs prevailed in urban areas. The particularities observed supported the purpose of this work and discriminated the contributions of various atmospheric pollution emission sources in lichen samples. A statistical approach based on principal component analysis (PCA) was applied and resolved these potential singularities into specific component factors. Even if a certain degree of mixing of the factors is pointed out, relevant relationships were observed with several atmospheric emission sources. By this methodology, the contribution of industrial emissions to the atmospheric metal, PAH, PCB, and PCDD/F levels was roughly estimated to be 60.2%, before biomass burning (10.2%) and road traffic (3.8%). These results demonstrate that lichen biomonitoring offers an encouraging perspective of spatially resolved source apportionment studies.

Amalgam Electrode-Based Electrochemical Detector for On-Site Direct Determination of Cadmium(II) and Lead(II) from Soils

PubMed Central

Nejdl, Lukas; Kynicky, Jindrich; Brtnicky, Martin; Vaculovicova, Marketa; Adam, Vojtech

2017-01-01

Toxic metal contamination of the environment is a global issue. In this paper, we present a low-cost and rapid production of amalgam electrodes used for determination of Cd(II) and Pb(II) in environmental samples (soils and wastewaters) by on-site analysis using difference pulse voltammetry. Changes in the electrochemical signals were recorded with a miniaturized potentiostat (width: 80 mm, depth: 54 mm, height: 23 mm) and a portable computer. The limit of detection (LOD) was calculated for the geometric surface of the working electrode 15 mm2 that can be varied as required for analysis. The LODs were 80 ng·mL−1 for Cd(II) and 50 ng·mL−1 for Pb(II), relative standard deviation, RSD ≤ 8% (n = 3). The area of interest (Dolni Rozinka, Czech Republic) was selected because there is a deposit of uranium ore and extreme anthropogenic activity. Environmental samples were taken directly on-site and immediately analysed. Duration of a single analysis was approximately two minutes. The average concentrations of Cd(II) and Pb(II) in this area were below the global average. The obtained values were verified (correlated) by standard electrochemical methods based on hanging drop electrodes and were in good agreement. The advantages of this method are its cost and time effectivity (approximately two minutes per one sample) with direct analysis of turbid samples (soil leach) in a 2 M HNO3 environment. This type of sample cannot be analyzed using the classical analytical methods without pretreatment. PMID:28792458

Extreme Environment Technologies for Space and Terrestrial Applications

NASA Technical Reports Server (NTRS)

Balint, Tibor S.; Cutts, James A.; Kolawa, Elizabeth A.; Peterson, Craig E.

2008-01-01

Over the next decades, NASA's planned solar system exploration missions are targeting planets, moons and small bodies, where spacecraft would be expected to encounter diverse extreme environmental (EE) conditions throughout their mission phases. These EE conditions are often coupled. For instance, near the surface of Venus and in the deep atmospheres of giant planets, probes would experience high temperatures and pressures. In the Jovian system low temperatures are coupled with high radiation. Other environments include thermal cycling, and corrosion. Mission operations could also introduce extreme conditions, due to atmospheric entry heat flux and deceleration. Some of these EE conditions are not unique to space missions; they can be encountered by terrestrial assets from the fields of defense,oil and gas, aerospace, and automotive industries. In this paper we outline the findings of NASA's Extreme Environments Study Team, including discussions on state of the art and emerging capabilities related to environmental protection, tolerance and operations in EEs. We will also highlight cross cutting EE mitigation technologies, for example, between high g-load tolerant impactors for Europa and instrumented projectiles on Earth; high temperature electronics sensors on Jupiter deep probes and sensors inside jet engines; and pressure vessel technologies for Venus probes and sea bottom monitors. We will argue that synergistic development programs between these fields could be highly beneficial and cost effective for the various agencies and industries. Some of these environments, however, are specific to space and thus the related technology developments should be spear headed by NASA with collaboration from industry and academia.

Sulfur 'Concrete' for Lunar Applications - Environmental Considerations

NASA Technical Reports Server (NTRS)

Grugel, R. N.

2008-01-01

Commercial use of sulfur concrete on Earth is well established, particularly in corrosive, e.g., acid and salt, environments. Having found troilite (FeS) on the Moon raises the question of using extracted sulfur as a lunar construction material, an attractive alternative to conventional concrete as it does not require water. For the purpose of this Technical Memorandum, it is assumed that lunar ore is mined, refined, and the raw sulfur processed with appropriate lunar regolith to form, for example, bricks. With this stipulation, it is then noted that the viability of sulfur concrete in a lunar environment, which is characterized by lack of an atmosphere and extreme temperatures, is not well understood. The work presented here evaluates two sets of small sulfur concrete samples that have been prepared using JSC-1 lunar simulant as an aggregate addition. One set was subjected to extended periods in high vacuum to evaluate sublimation issues, and the other was cycled between room and liquid nitrogen temperatures to investigate their subsequent mechanical integrity. Results are presented from both investigations, discussed, and put into the context of the lunar environment.

In Situ Solid Particle Generator

NASA Technical Reports Server (NTRS)

Agui, Juan H.; Vijayakumar, R.

2013-01-01

Particle seeding is a key diagnostic component of filter testing and flow imaging techniques. Typical particle generators rely on pressurized air or gas sources to propel the particles into the flow field. Other techniques involve liquid droplet atomizers. These conventional techniques have drawbacks that include challenging access to the flow field, flow and pressure disturbances to the investigated flow, and they are prohibitive in high-temperature, non-standard, extreme, and closed-system flow conditions and environments. In this concept, the particles are supplied directly within a flow environment. A particle sample cartridge containing the particles is positioned somewhere inside the flow field. The particles are ejected into the flow by mechanical brush/wiper feeding and sieving that takes place within the cartridge chamber. Some aspects of this concept are based on established material handling techniques, but they have not been used previously in the current configuration, in combination with flow seeding concepts, and in the current operational mode. Unlike other particle generation methods, this concept has control over the particle size range ejected, breaks up agglomerates, and is gravity-independent. This makes this device useful for testing in microgravity environments.

Isolation of hydrocarbon-degrading extremely halophilic archaea from an uncontaminated hypersaline pond (Camargue, France).

PubMed

Tapilatu, Yosmina H; Grossi, Vincent; Acquaviva, Monique; Militon, Cécile; Bertrand, Jean-Claude; Cuny, Philippe

2010-03-01

Little information exists about the ability of halophilic archaea present in hypersaline environments to degrade hydrocarbons. In order to identify the potential actors of hydrocarbon degradation in these environments, enrichment cultures were prepared using samples collected from a shallow crystallizer pond with no known contamination history in Camargue, France, with n-alkanes provided as source of carbon and energy. Five alkane-degrading halophilic archaeal strains were isolated: one (strain MSNC 2) was closely related to Haloarcula and three (strains MSNC 4, MSNC 14, and MSNC 16) to Haloferax. Biodegradation assays showed that depending on the strain, 32 to 95% (0.5 g/l) of heptadecane was degraded after 30 days of incubation at 40 degrees C in 225 g/l NaCl artificial medium. One of the strains (MSNC 14) was also able to degrade phenanthrene. This work clearly shows for the first time the potential role of halophilic archaea belonging to the genera Haloarcula and Haloferax in the degradation of hydrocarbons in both pristine and hydrocarbon-contaminated hypersaline environments.

Radiation-Induced Damage and Recovery of Ultra-Nanocrystalline Diamond: Toward Applications in Harsh Environments

DOE PAGES

Martin, Aiden A.; Filevich, Jorge; Straw, Marcus; ...

2017-10-23

Ultra-nanocrystalline diamond (UNCD) is increasingly being used in the fabrication of devices and coatings due to its excellent tribological properties, corrosion resistance, and biocompatibility. Here in this work, we study its response to irradiation with kiloelectronvolt electrons as a controlled model for extreme ionizing environments. Real time Raman spectroscopy reveals that the radiation-damage mechanism entails dehydrogenation of UNCD grain boundaries, and we show that the damage can be recovered by annealing at 883 K. Lastly, our results have significant practical implications for the implementation of UNCD in extreme environment applications, and indicate that the films can be used as radiationmore » sensors.« less

Radiation-Induced Damage and Recovery of Ultra-Nanocrystalline Diamond: Toward Applications in Harsh Environments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martin, Aiden A.; Filevich, Jorge; Straw, Marcus

Ultra-nanocrystalline diamond (UNCD) is increasingly being used in the fabrication of devices and coatings due to its excellent tribological properties, corrosion resistance, and biocompatibility. Here in this work, we study its response to irradiation with kiloelectronvolt electrons as a controlled model for extreme ionizing environments. Real time Raman spectroscopy reveals that the radiation-damage mechanism entails dehydrogenation of UNCD grain boundaries, and we show that the damage can be recovered by annealing at 883 K. Lastly, our results have significant practical implications for the implementation of UNCD in extreme environment applications, and indicate that the films can be used as radiationmore » sensors.« less

Can we colonize the solar system? Human biology and survival in the extreme space environment.

PubMed

Launius, Roger D

2010-09-01

Throughout the history of the space age the dominant vision for the future has been great spaceships plying the solar system, and perhaps beyond, moving living beings from one planet to another. Spacesuited astronauts would carry out exploration, colonization, and settlement as part of a relentlessly forward looking movement of humanity beyond Earth. As time has progressed this image has not changed appreciably even as the full magnitude of the challenges it represents have become more and more apparent. This essay explores the issues associated with the human movement beyond Earth and raises questions about whether humanity will ever be able to survive in the extreme environment of space and the other bodies of the solar system. This paper deals with important historical episodes as well as wider conceptual issues about life in space. Two models of expansion beyond Earth are discussed: (1) the movement of microbes and other types of life on Earth that can survive the space environment and (2) the modification of humans into cyborgs for greater capability to survive in the extreme environments encountered beyond this planet. (c) 2010 Elsevier Ltd. All rights reserved.

Characterization of the microbial community composition and the distribution of Fe-metabolizing bacteria in a creek contaminated by acid mine drainage.

PubMed

Sun, Weimin; Xiao, Enzong; Krumins, Valdis; Dong, Yiran; Xiao, Tangfu; Ning, Zengping; Chen, Haiyan; Xiao, Qingxiang

2016-10-01

A small watershed heavily contaminated by long-term acid mine drainage (AMD) from an upstream abandoned coal mine was selected to study the microbial community developed in such extreme system. The watershed consists of AMD-contaminated creek, adjacent contaminated soils, and a small cascade aeration unit constructed downstream, which provide an excellent contaminated site to study the microbial response in diverse extreme AMD-polluted environments. The results showed that the innate microbial communities were dominated by acidophilic bacteria, especially acidophilic Fe-metabolizing bacteria, suggesting that Fe and pH are the primary environmental factors in governing the indigenous microbial communities. The distribution of Fe-metabolizing bacteria showed distinct site-specific patterns. A pronounced shift from diverse communities in the upstream to Proteobacteria-dominated communities in the downstream was observed in the ecosystem. This location-specific trend was more apparent at genus level. In the upstream samples (sampling sites just below the coal mining adit), a number of Fe(II)-oxidizing bacteria such as Alicyclobacillus spp., Metallibacterium spp., and Acidithrix spp. were dominant, while Halomonas spp. were the major Fe(II)-oxidizing bacteria observed in downstream samples. Additionally, Acidiphilium, an Fe(III)-reducing bacterium, was enriched in the upstream samples, while Shewanella spp. were the dominant Fe(III)-reducing bacteria in downstream samples. Further investigation using linear discriminant analysis (LDA) effect size (LEfSe), principal coordinate analysis (PCoA), and unweighted pair group method with arithmetic mean (UPGMA) clustering confirmed the difference of microbial communities between upstream and downstream samples. Canonical correspondence analysis (CCA) and Spearman's rank correlation indicate that total organic carbon (TOC) content is the primary environmental parameter in structuring the indigenous microbial communities, suggesting that the microbial communities are shaped by three major environmental parameters (i.e., Fe, pH, and TOC). These findings were beneficial to a better understanding of natural attenuation of AMD.

Summary of Research Issues in Behavior and Performance in Isolated and Confined Extreme (ICE) Environments

NASA Technical Reports Server (NTRS)

Palinkas, Lawrence A.

2000-01-01

The papers presented in this section describe changes in behavior and performance in various isolated and confined extreme (ICE) environments, including Antarctic expeditions and research stations, space simulators and isolation chambers, and submarines. Each of these environments possesses characteristics that are in some way analogous to those found on long-duration space missions. Despite differences in length of mission, characteristics of mission personnel or crew, and characteristics in the physical environment, the various ICE environments described in this collection of papers appear to produce similar changes in behavior and performance. These changes include increased disturbances of mood, increased rates of psychiatric disorder, increased interpersonal tension, and a disruption of circadian rhythms. However, these environments do not inherently produce decrements in performance. Palinkas and colleagues suggest that prolonged exposure to the isolation and confinement in the Antarctic can actually have positive or "salutogenic" effects as well, evidenced by a decrease in mood disturbances and increase in performance measures.

Integration of modern statistical tools for the analysis of climate extremes into the web-GIS “CLIMATE”

NASA Astrophysics Data System (ADS)

Ryazanova, A. A.; Okladnikov, I. G.; Gordov, E. P.

2017-11-01

The frequency of occurrence and magnitude of precipitation and temperature extreme events show positive trends in several geographical regions. These events must be analyzed and studied in order to better understand their impact on the environment, predict their occurrences, and mitigate their effects. For this purpose, we augmented web-GIS called “CLIMATE” to include a dedicated statistical package developed in the R language. The web-GIS “CLIMATE” is a software platform for cloud storage processing and visualization of distributed archives of spatial datasets. It is based on a combined use of web and GIS technologies with reliable procedures for searching, extracting, processing, and visualizing the spatial data archives. The system provides a set of thematic online tools for the complex analysis of current and future climate changes and their effects on the environment. The package includes new powerful methods of time-dependent statistics of extremes, quantile regression and copula approach for the detailed analysis of various climate extreme events. Specifically, the very promising copula approach allows obtaining the structural connections between the extremes and the various environmental characteristics. The new statistical methods integrated into the web-GIS “CLIMATE” can significantly facilitate and accelerate the complex analysis of climate extremes using only a desktop PC connected to the Internet.

The scaling of population persistence with carrying capacity does not asymptote in populations of a fish experiencing extreme climate variability.

PubMed

White, Richard S A; Wintle, Brendan A; McHugh, Peter A; Booker, Douglas J; McIntosh, Angus R

2017-06-14

Despite growing concerns regarding increasing frequency of extreme climate events and declining population sizes, the influence of environmental stochasticity on the relationship between population carrying capacity and time-to-extinction has received little empirical attention. While time-to-extinction increases exponentially with carrying capacity in constant environments, theoretical models suggest increasing environmental stochasticity causes asymptotic scaling, thus making minimum viable carrying capacity vastly uncertain in variable environments. Using empirical estimates of environmental stochasticity in fish metapopulations, we showed that increasing environmental stochasticity resulting from extreme droughts was insufficient to create asymptotic scaling of time-to-extinction with carrying capacity in local populations as predicted by theory. Local time-to-extinction increased with carrying capacity due to declining sensitivity to demographic stochasticity, and the slope of this relationship declined significantly as environmental stochasticity increased. However, recent 1 in 25 yr extreme droughts were insufficient to extirpate populations with large carrying capacity. Consequently, large populations may be more resilient to environmental stochasticity than previously thought. The lack of carrying capacity-related asymptotes in persistence under extreme climate variability reveals how small populations affected by habitat loss or overharvesting, may be disproportionately threatened by increases in extreme climate events with global warming. © 2017 The Author(s).

Survival of extreme opinions

NASA Astrophysics Data System (ADS)

Hsu, Jiann-wien; Huang, Ding-wei

2009-12-01

We study the survival of extreme opinions in various processes of consensus formation. All the opinions are treated equally and subjected to the same rules of changing. We investigate three typical models to reach a consensus in each case: (A) personal influence, (B) influence from surroundings, and (C) influence to surroundings. Starting with uniformly distributed random opinions, our calculated results show that the extreme opinions can survive in both models (A) and (B), but not in model (C). We obtain a conclusion that both personal influence and passive adaptation to the environment are not sufficient enough to eradicate all the extreme opinions. Only the active persuasion to change the surroundings eliminates the extreme opinions completely.

Using Upper Extremity Skin Temperatures to Assess Thermal Comfort in Office Buildings in Changsha, China

PubMed Central

Wu, Zhibin; Li, Nianping; Cui, Haijiao; Peng, Jinqing; Chen, Haowen; Liu, Penglong

2017-01-01

Existing thermal comfort field studies are mainly focused on the relationship between the indoor physical environment and the thermal comfort. In numerous chamber experiments, physiological parameters were adopted to assess thermal comfort, but the experiments’ conclusions may not represent a realistic thermal environment due to the highly controlled thermal environment and few occupants. This paper focuses on determining the relationships between upper extremity skin temperatures (i.e., finger, wrist, hand and forearm) and the indoor thermal comfort. Also, the applicability of predicting thermal comfort by using upper extremity skin temperatures was explored. Field studies were performed in office buildings equipped with split air-conditioning (SAC) located in the hot summer and cold winter (HSCW) climate zone of China during the summer of 2016. Psychological responses of occupants were recorded and physical and physiological factors were measured simultaneously. Standard effective temperature (SET*) was used to incorporate the effect of humidity and air velocity on thermal comfort. The results indicate that upper extremity skin temperatures are good indicators for predicting thermal sensation, and could be used to assess the thermal comfort in terms of physiological mechanism. In addition, the neutral temperature was 24.7 °C and the upper limit for 80% acceptability was 28.2 °C in SET*. PMID:28934173

Using Upper Extremity Skin Temperatures to Assess Thermal Comfort in Office Buildings in Changsha, China.

PubMed

Wu, Zhibin; Li, Nianping; Cui, Haijiao; Peng, Jinqing; Chen, Haowen; Liu, Penglong

2017-09-21

Existing thermal comfort field studies are mainly focused on the relationship between the indoor physical environment and the thermal comfort. In numerous chamber experiments, physiological parameters were adopted to assess thermal comfort, but the experiments' conclusions may not represent a realistic thermal environment due to the highly controlled thermal environment and few occupants. This paper focuses on determining the relationships between upper extremity skin temperatures (i.e., finger, wrist, hand and forearm) and the indoor thermal comfort. Also, the applicability of predicting thermal comfort by using upper extremity skin temperatures was explored. Field studies were performed in office buildings equipped with split air-conditioning (SAC) located in the hot summer and cold winter (HSCW) climate zone of China during the summer of 2016. Psychological responses of occupants were recorded and physical and physiological factors were measured simultaneously. Standard effective temperature (SET*) was used to incorporate the effect of humidity and air velocity on thermal comfort. The results indicate that upper extremity skin temperatures are good indicators for predicting thermal sensation, and could be used to assess the thermal comfort in terms of physiological mechanism. In addition, the neutral temperature was 24.7 °C and the upper limit for 80% acceptability was 28.2 °C in SET*.

Lack of correlation of desiccation and radiation tolerance in microorganisms from diverse extreme environments tested under anoxic conditions

PubMed Central

Bohmeier, Maria; Perras, Alexandra K; Schwendner, Petra; Rabbow, Elke; Moissl-Eichinger, Christine; Cockell, Charles S; Vannier, Pauline; Marteinsson, Viggo T; Monaghan, Euan P; Ehrenfreund, Pascale; Garcia-Descalzo, Laura; Gómez, Felipe; Malki, Moustafa; Amils, Ricardo; Gaboyer, Frédéric; Westall, Frances; Cabezas, Patricia; Walter, Nicolas; Rettberg, Petra

2018-01-01

Abstract Four facultative anaerobic and two obligate anaerobic bacteria were isolated from extreme environments (deep subsurface halite mine, sulfidic anoxic spring, mineral-rich river) in the frame MASE (Mars Analogues for Space Exploration) project. The isolates were investigated under anoxic conditions for their survivability after desiccation up to 6 months and their tolerance to ionizing radiation up to 3000 Gy. The results indicated that tolerances to both stresses are strain-specific features. Yersinia intermedia MASE-LG-1 showed a high desiccation tolerance but its radiation tolerance was very low. The most radiation-tolerant strains were Buttiauxella sp. MASE-IM-9 and Halanaerobium sp. MASE-BB-1. In both cases, cultivable cells were detectable after an exposure to 3 kGy of ionizing radiation, but cells only survived desiccation for 90 and 30 days, respectively. Although a correlation between desiccation and ionizing radiation resistance has been hypothesized for some aerobic microorganisms, our data showed that there was no correlation between tolerance to desiccation and ionizing radiation, suggesting that the physiological basis of both forms of tolerances is not necessarily linked. In addition, these results indicated that facultative and obligate anaerobic organisms living in extreme environments possess varied species-specific tolerances to extremes. PMID:29474542

The Characterization of Psychrophilic Microorganisms and their potentially useful Cold-Active Glycosidases Final Progress Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brenchly, Jean E.

Our studies of novel, cold-loving microorganisms have focused on two distinct extreme environments. The first is an ice core sample from a 120,000 year old Greenland glacier. The results of this study are particularly exciting and have been highlighted with press releases and additional coverage. The first press release in 2004 was based on our presentation at the General Meeting of the American Society for Microbiology and was augmented by coverage of our publication (Appl. Environ. Microbiol. 2005. Vol. 71:7806) in the Current Topics section of the ASM news journal, “Microbe.” Of special interest for this report was the isolationmore » of numerous, phylogenetically distinct and potentially novel ultrasmall microorganisms. The detection and isolation of members of the ultrasmall population is significant because these cells pass through 0.2 micron pore filters that are generally used to trap microorganisms from environmental samples. Thus, analyses by other investigators that examined only cells captured on the filters would have missed a significant portion of this population. Only a few ultrasmall isolates had been obtained prior to our examination of the ice core samples. Our development of a filtration enrichment and subsequent cultivation of these organisms has added extensively to the collection of, and knowledge about, this important population in the microbial world.« less

Mediation of seed provisioning in the transmission of environmental maternal effects in Maritime pine (Pinus pinaster Aiton).

PubMed

Zas, R; Cendán, C; Sampedro, L

2013-09-01

Although maternal environmental effects are increasingly recognized as an important source of phenotypic variation with relevant impacts in evolutionary processes, their relevance in long-lived plants such as pine trees is largely unknown. Here, we used a powerful sample size and a strong quantitative genetic approach to analyse the sources of variation of early seedling performance and to identify seed mass (SM)-dependent and -independent maternal environmental effects in Maritime pine. We measured SM of 8924 individual seeds collected from 10 genotypes clonally replicated in two environments of contrasting quality (favourable and stressful), and we measured seedling growth rate and biomass allocation to roots and shoots. SM was extremely variable (up to 14-fold) and strongly determined by the maternal environment and the genotype of the mother tree. The favourable maternal environment led to larger cones, larger seeds and reduced SM variability. The maternal environment also determined the offspring phenotype, with seedlings coming from the favourable environment being 35% larger and with greater root/shoot ratio. Transgenerational plasticity appears, thus, to be a relevant source of phenotypic variation in the early performance of this pine species. Seed provisioning explained most of the effect of the maternal environment on seedling total biomass. Environmental maternal effects on seedling biomass allocation were, however, determined through SM-independent mechanisms, suggesting that other epigenetic regulation channels may be involved.

Mediation of seed provisioning in the transmission of environmental maternal effects in Maritime pine (Pinus pinaster Aiton)

PubMed Central

Zas, R; Cendán, C; Sampedro, L

2013-01-01

Although maternal environmental effects are increasingly recognized as an important source of phenotypic variation with relevant impacts in evolutionary processes, their relevance in long-lived plants such as pine trees is largely unknown. Here, we used a powerful sample size and a strong quantitative genetic approach to analyse the sources of variation of early seedling performance and to identify seed mass (SM)-dependent and -independent maternal environmental effects in Maritime pine. We measured SM of 8924 individual seeds collected from 10 genotypes clonally replicated in two environments of contrasting quality (favourable and stressful), and we measured seedling growth rate and biomass allocation to roots and shoots. SM was extremely variable (up to 14-fold) and strongly determined by the maternal environment and the genotype of the mother tree. The favourable maternal environment led to larger cones, larger seeds and reduced SM variability. The maternal environment also determined the offspring phenotype, with seedlings coming from the favourable environment being 35% larger and with greater root/shoot ratio. Transgenerational plasticity appears, thus, to be a relevant source of phenotypic variation in the early performance of this pine species. Seed provisioning explained most of the effect of the maternal environment on seedling total biomass. Environmental maternal effects on seedling biomass allocation were, however, determined through SM-independent mechanisms, suggesting that other epigenetic regulation channels may be involved. PMID:23652562

ALMA observation of high-z extreme star-forming environments discovered by Planck/Herschel

NASA Astrophysics Data System (ADS)

Kneissl, R.

2015-05-01

The Comic Microwave Background satellite Planck with its High Frequency Instrument has surveyed the mm/sub-mm sky in six frequency channels from 100 to 900 GHz. A sample of 228 cold sources of the Cosmic Infrared Background was observed in follow-up with Herschel SPIRE. The majority of sources appear to be over-densities of star-forming galaxies matching the size of high-z proto-cluster regions, while a 3% fraction are individual bright, lensed galaxies. A large observing program is underway with the aim of resolving the regions into the constituent members of the Planck sources. First ALMA data have been received on one Planck/Herschel proto-cluster candidate, showing the expected large over-abundance of bright mm/sub-mm sources within the cluster region. ALMA long baseline data of the brightest lensed galaxy in the sample with > 1 Jy at 350 μm are also forthcoming.

Shining a light on planetary processes using synchrotron techniques

NASA Astrophysics Data System (ADS)

Brand, H. E. A.; Kimpton, J. A.

2017-12-01

The Australian Synchrotron is a world-class national research facility that uses accelerator technology to produce X-rays and infrared for research. It is available for researchers from all institutions and disciplines. This contribution is intended to inform the community of the current capabilities at the facility using examples drawn from planetary research across the beamlines. Examples will include: formation of jarosite minerals with a view to Mars; studies of Micrometeorites; and large volume CT imaging of geological samples. A suite of new beamlines has been proposed for the growth of the facility and one of these, ADS, the Advanced Diffraction and Scattering beamline, is intended to be a high energy X-ray diffraction beamline capable of reaching extreme conditions and carrying out challenging in situ experiments. There is an opportunity to develop complex new sample environments which could be of relevance to shock metamorphic processes and this will form part of the discussion.

Science at the Theatre - Extreme Science - Promo Video

ScienceCinema

Klein, Spencer

2017-12-12

On Feb. 27 at 7 pm at the Berkeley Repertory Theatre, join four Berkeley Lab scientists as they discuss extreme science -- and what it means to you. Topics include: Neutrino hunting in Antarctica. Learn why Spencer Klein goes to the ends of the Earth to search for these ghostly particles. From Chernobyl to Central Asia, Tamas Torok travels the globe to study microbial diversity in extreme environments. Andrew Minor uses the world's most advanced electron microscopes to explore materials at ultrahigh stresses and in harsh environments. And microbes that talk to computers? Caroline Ajo-Franklin is pioneering cellular-electrical connections that could help transform sunlight into fuel. Go here for more information and to view videos of previous Science at the Theater events: http://www.lbl.gov/LBL-PID/fobl/

Science at the Theatre - Extreme Science - Promo Video

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klein, Spencer

On Feb. 27 at 7 pm at the Berkeley Repertory Theatre, join four Berkeley Lab scientists as they discuss extreme science -- and what it means to you. Topics include: Neutrino hunting in Antarctica. Learn why Spencer Klein goes to the ends of the Earth to search for these ghostly particles. From Chernobyl to Central Asia, Tamas Torok travels the globe to study microbial diversity in extreme environments. Andrew Minor uses the world's most advanced electron microscopes to explore materials at ultrahigh stresses and in harsh environments. And microbes that talk to computers? Caroline Ajo-Franklin is pioneering cellular-electrical connections thatmore » could help transform sunlight into fuel. Go here for more information and to view videos of previous Science at the Theater events: http://www.lbl.gov/LBL-PID/fobl/« less

Definition of exobiology experiments for future Mars missions

NASA Technical Reports Server (NTRS)

Mancinelli, Rocco L.

1996-01-01

During the past year we have concentrated on two objectives. The first objective is ongoing and is to define the experimental parameters that are necessary to conduct autonomously a mineralogical analysis of the Martian surface in situ using differential thermal analysis coupled with gas chromatography (DTA/GC). The rationale in support of this objective is that proper interpretation of the mineralogical data from the DTA/GC can be used to better describe the present and past environments of Mars, leading to a better assessment of the probability of life evolving on Mars. To meet these objectives we have analyzed a number of samples collected from nature using the DTA/GC. One of the more significant findings was that in samples of desert varnish we detected magnetite and maghemite that may serve as potential biomarkers applicable to DTA/GC analyses of Martian surface material during landed missions. The second objective follows from the first and is to better understand microbe-environment interactions by determining the response of microbes to changes in their environment, including extreme desiccation and solar UV-radiation. The rationale behind this is to develop hypotheses regarding what may have happened to life that may have arose on Mars, and microbial life that may get to the surface of Mars via spacecraft, or meteors from Earth. To accomplish this objective we have exposed microbes, collected from NaCl and gypsum-halite crystals, to the space environment aboard the ESA-German Biopan facility for 15 days. The most significant finding was that these microbes survived the exposure better than others.

A novel thermophilic methane-oxidizing bacteria from thermal springs of Uzon volcano caldera, Kamchatka

NASA Astrophysics Data System (ADS)

Dvorianchikova, E.; Kizilova, A.; Kravchenko, I.; Galchenko, V.

2012-04-01

Methane is a radiatively active trace gas, contributing significantly to the greenhouse effect. It is 26 times more efficient in absorbing and re-emitting infrared radiation than carbon dioxide. Methanotrophs play an essential role in the global carbon cycle by oxidizing 50-75% of the biologically produced methane in situ, before it reaches the atmosphere. Methane-oxidizing bacteria are isolated from the various ecosystems and described at present. Their biology, processes of methane oxidation in fresh-water, marsh, soil and marine habitats are investigated quite well. Processes of methane oxidation in places with extreme physical and chemical conditions (high or low , salinity and temperature values) are studied in much smaller degree. Such ecosystems occupy a considerable part of the Earth's surface. The existence of aerobic methanotrophs inhabiting extreme environments has been verified so far by cultivation experiments and direct detection of methane monooxygenase genes specific to almost all aerobic methanotrophs. Thermophilic and thermotolerant methanotrophs have been isolated from such extreme environments and consist of the gammaproteobacterial (type I) genera Methylothermus, Methylocaldum, Methylococcus and the verrucomicrobial genus Methylacidiphilum. Uzon volcano caldera is a unique area, where volcanic processes still happen today. Hydrothermal springs of the area are extreme ecosystems which microbial communities represent considerable scientific interest of fundamental and applied character. A thermophilic aerobic methane-oxidising bacterium was isolated from a sediment sample from a hot spring (56.1; 5.3) of Uzon caldera. Strain S21 was isolated using mineral low salt medium. The headspace gas was composed of CH4, Ar, CO2, and O2 (40:40:15:5). The temperature of cultivation was 50, pH 5.5. Cells of strain S21 in exponential and early-stationary phase were coccoid bacilli, about 1 μm in diameter, and motile with a single polar flagellum. PCR and molecular cloning of a pmoA gene fragment have shown that strain S21 was moderately related to the genus Methylothermus; the closest organism is Methylothermus subterraneus. The further studying of strain S21 will expand our knowledge of this group of organisms, important from the ecological point of view.

The Central Molecular Zone of the Milky Way: Lessons about Star Formation from an extreme Environment

NASA Astrophysics Data System (ADS)

Kauffmann, Jens; Thushara Pillai, G. S.; Zhang, Qizhou; Lu, Xing; Immer, Katharina

2015-08-01

The Central Molecular Zone of the Milky Way (CMZ; innermost ~100pc) hosts a number of remarkably dense and massive clouds. These are subject to extreme environmental conditions, including very high cosmic ray fluxes and strong magnetic fields. Exploring star formation under such exceptional circumstances is essential for several of reasons. First, the CMZ permits to probe an extreme point in the star formation parameter space, which helps to test theoretical models. Second, CMZ clouds might help to understand the star formation under extreme conditions in more distant environments, such as in starbursts and the early universe.One particularly striking aspect is that — compared to the solar neighborhood — CMZ star formation in dense gas is suppressed by more than an order of magnitude (Longmore et al. 2012, Kauffmann et al. 2013). This questions current explanations for relations between the dense gas and the star formation rate (e.g., Gao & Solomon 2004, Lada et al. 2012). In other words, the unusually dense and massive CMZ molecular clouds form only very few stars, if any at all. Why is this so?Based on data from ALMA, CARMA, and SMA interferometers, we present results from the Galactic Center Molecular Cloud Survey (GCMS), the first study of a comprehensive sample of molecular clouds in the CMZ. This research yields a curious result: most of the major CMZ clouds are essentially devoid of significant substructure of the sort usually found in regions of high-mass star formation (Kauffmann et al. 2013). Preliminary analysis indicates that some clouds rather resemble homogeneous balls of gas. This suggests a highly dynamic picture of cloud evolution in the CMZ where clouds form, disperse, and re-assemble constantly. This concept is benchmarked against a new ALMA survey and first results from a legacy survey on the SMA.It is plausible that dense clouds in other galaxies have a similar internal structure. Instruments like ALMA and the JWST will soon permit to resolve such regions in nearby galaxies.

NASA Extreme Environments Mission Operations 10 - Evaluation of Robotic and Sensor Technologies for Surgery in Extreme Environments

DTIC Science & Technology

2006-11-01

ExPOC), LinkQUest, and working w/ ExPOC, go to the known locations identified in the site survey exercise. 1. Does the direction a diver is...map/ survey an area MD-5 7 April 06 08:30 & 14:20 hrs MD-9 08:15 Prior to EVA surface exploration, robotic rovers could potentially identify...What ROV tasks can be controlled from the ground and what have to be controlled from w/in the habitat SITE SURVEY ROV Objectives (all on

Spatiotemporal influence of temperature, air quality, and urban environment on cause-specific mortality during hazy days.

PubMed

Ho, Hung Chak; Wong, Man Sing; Yang, Lin; Shi, Wenzhong; Yang, Jinxin; Bilal, Muhammad; Chan, Ta-Chien

2018-03-01

Haze is an extreme weather event that can severely increase air pollution exposure, resulting in higher burdens on human health. Few studies have explored the health effects of haze, and none have investigated the spatiotemporal interaction between temperature, air quality and urban environment that may exacerbate the adverse health effects of haze. We investigated the spatiotemporal pattern of haze effects and explored the additional effects of temperature, air pollution and urban environment on the short-term mortality risk during hazy days. We applied a Poisson regression model to daily mortality data from 2007 through 2014, to analyze the short-term mortality risk during haze events in Hong Kong. We evaluated the adverse effect on five types of cause-specific mortality after four types of haze event. We also analyzed the additional effect contributed by the spatial variability of urban environment on each type of cause-specific mortality during a specific haze event. A regular hazy day (lag 0) has higher all-cause mortality risk than a day without haze (odds ratio: 1.029 [1.009, 1.049]). We have also observed high mortality risks associated with mental disorders and diseases of the nervous system during hazy days. In addition, extreme weather and air quality contributed to haze-related mortality, while cold weather and higher ground-level ozone had stronger influences on mortality risk. Areas with a high-density environment, lower vegetation, higher anthropogenic heat, and higher PM 2.5 featured stronger effects of haze on mortality than the others. A combined influence of haze, extreme weather/air quality, and urban environment can result in extremely high mortality due to mental/behavioral disorders or diseases of the nervous system. In conclusion, we developed a data-driven technique to analyze the effects of haze on mortality. Our results target the specific dates and areas with higher mortality during haze events, which can be used for development of health warning protocols/systems. Copyright © 2017 Elsevier Ltd. All rights reserved.

[Microbial diversity and ammonia-oxidizing microorganism of a soil sample near an acid mine drainage lake].

PubMed

Liu, Ying; Wang, Li-Hua; Hao, Chun-Bo; Li, Lu; Li, Si-Yuan; Feng, Chuan-Ping

2014-06-01

The main physicochemical parameters of the soil sample which was collected near an acid mine drainage reservoir in Anhui province was analyzed. The microbial diversity and community structure was studied through the construction of bacteria and archaea 16S rRNA gene clone libraries and ammonia monooxygenase gene clone library of archaea. The functional groups which were responsible for the process of ammonia oxidation were also discussed. The results indicated that the soil sample had extreme low pH value (pH < 3) and high ions concentration, which was influenced by the acid mine drainage (AMD). All the 16S rRNA gene sequences of bacteria clone library fell into 11 phyla, and Acidobacteria played the most significant role in the ecosystem followed by Verrucomicrobia. A great number of acidophilic bacteria existed in the soil sample, such as Candidatus Koribacter versatilis and Holophaga sp.. The archaea clone library consisted of 2 phyla (Thaumarchaeota and Euryarchaeota). The abundance of Thaumarchaeota was remarkably higher than Euryarchaeota. The ammonia oxidation in the soil environment was probably driven by ammonia-oxidizing archaea, and new species of ammonia-oxidizing archaea existed in the soil sample.

Mapping Extreme Heat Vulnerability and Health Outcomes to inform the District of Columbia's Climate Adaptation Plan

NASA Astrophysics Data System (ADS)

Declet-Barreto, J.; Wilhelmi, O.; Goggans, A.

2016-12-01

In this collaborative engagement, scientists are partnering with the District of Columbia (DC) to develop an extreme heat vulnerability assessment. To do so, we map socio-demographic and built environment indicators of extreme heat vulnerability in Census Tracts in DC neighborhoods. In order to provide information useful for DC public health and urban planning practitioners, we aggregate the indicators into an index of extreme heat vulnerability. We compare the index against heat-related call data from DC's 911 system to better understand the socio-spatial distribution of extreme heat-related health outcomes. Our assessment can help inform the District's Climate Adaptation Plan as well as increase public engagement in reducing vulnerability to extreme heat.

A Motor Drive Electronics Assembly for Mars Curiosity Rover: An Example of Assembly Qualification for Extreme Environments

NASA Technical Reports Server (NTRS)

Kolawa, Elizabeth; Chen, Yuan; Mojarradi, Mohammad M.; Tudryn Weber, Carissa

2013-01-01

In this paper, the technology development and infusion of the motor drive electronics assembly, along with the technology qualification and space qualification, is described and detailed. The process is an example of the qualification methodology for extreme environmen

Focusing adaptive-optics for neutron spectroscopy at extreme conditions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Simeoni, G. G., E-mail: ggsimeoni@outlook.com; Physics Department E13, Technical University of Munich, D-85748 Garching; Valicu, R. G.

2015-12-14

Neutron Spectroscopy employing extreme-conditions sample environments is nowadays a crucial tool for the understanding of fundamental scientific questions as well as for the investigation of materials and chemical-physical properties. For all these kinds of studies, an increased neutron flux over a small sample area is needed. The prototype of a focusing neutron guide component, developed and produced completely at the neutron source FRM II in Garching (Germany), has been installed at the time-of-flight (TOF) disc-chopper neutron spectrometer TOFTOF and came into routine-operation. The design is based on the compressed Archimedes' mirror concept for finite-size divergent sources. It represents a uniquemore » device combining the supermirror technology with Adaptive Optics, suitable for broad-bandwidth thermal-cold TOF neutron spectroscopy (here optimized for 1.4–10 Å). It is able to squeeze the beam cross section down to a square centimeter, with a more than doubled signal-to-background ratio, increased efficiency at high scattering angles, and improved symmetry of the elastic resolution function. We present a comparison between the simulated and measured beam cross sections, as well as the performance of the instrument within real experiments. This work intends to show the unprecedented opportunities achievable at already existing instruments, along with useful guidelines for the design and construction of next-generation neutron spectrometers.« less

Viruses in Antarctic lakes

NASA Technical Reports Server (NTRS)

Kepner, R. L. Jr; Wharton, R. A. Jr; Suttle, C. A.; Wharton RA, J. r. (Principal Investigator)

1998-01-01

Water samples collected from four perennially ice-covered Antarctic lakes during the austral summer of 1996-1997 contained high densities of extracellular viruses. Many of these viruses were found to be morphologically similar to double-stranded DNA viruses that are known to infect algae and protozoa. These constitute the first observations of viruses in perennially ice-covered polar lakes. The abundance of planktonic viruses and data suggesting substantial production potential (relative to bacteria] secondary and photosynthetic primary production) indicate that viral lysis may be a major factor in the regulation of microbial populations in these extreme environments. Furthermore, we suggest that Antarctic lakes may be a reservoir of previously undescribed viruses that possess novel biological and biochemical characteristics.

Oxygen isotope values of tree ring α-cellulose as a proxy of hydroclimate variability in arid regions

NASA Astrophysics Data System (ADS)

Dodd, J. P.; Freimuth, E. J.; Olson, E. J.; Diefendorf, A. F.

2015-12-01

One of the main goals of tree ring isotope studies is to reconstruct climate-driven variations in the source water and antecedent precipitation; however, evaporation in the soil and leaves can significantly modify the isotope values of the source water. This is particularly the case in arid environments where evaporative effects are perhaps the most significant unknown variable when attempting to reconstruct regional-scale hydroclimate variations from tree ring isotope proxies. To quantify the effects of extreme aridity on α-cellulose δ18O values, we measured the oxygen isotope values of groundwater, xylem water, leaf water, and tree ring α-cellulose in an endemic species of drought-resistant trees (Prosopis tamarugo) from different microenvironments throughout the Atacama Desert of Northern Chile. Average annual precipitation is <5 mm/yr, and groundwater is the primary water source for P. tamarugo trees in the region. Groundwater δ18O values at the sample locations range from -6.7 to -9.7‰, and xylem water δ18O values record a systematic increase (ave. Δ18Ox-gw =+1.3‰; 2σ =1.0‰). Leaf waters are significantly affected by evaporative enrichment with a range of δ18O values from 7 to 23‰. This range most likely reflects a number of physiological and environmental conditions including tree size, canopy development, and sample time (i.e. morning vs. evening). However, despite the large variation in leaf water δ18O values, the average difference between the α-cellulose and groundwater is very consistent (Δ18Oc-gw = +39.7‰; 2σ =1.3‰). P. tamarugo samples were collected in austral spring, when tree growth was at its maximum; therefore, any seasonal variations in plant physiology not captured with this dataset will have a limited impact on cellulose production. These data demonstrate that despite the variable evaporative enrichment of 18O in the leaf water, the α-cellulose δ18O values provide a remarkably consistent record of variations in groundwater δ18O values in this extremely arid environment.

Terrestrial Environment (Climatic) Criteria Guidelines for Use in Aerospace Vehicle Development, 1973 Revision

NASA Technical Reports Server (NTRS)

Daniels, G. E. (Editor)

1973-01-01

Guidelines are provided on probable climatic extremes and terrestrial environment data applicable to space vehicle and associated equipment design and development. Operational criteria for ground support sites are emphasized.

The influence of a rapid drawdown and prolonged dewatering on angling pressure, catch and harvest in a Nebraska reservoir

USGS Publications Warehouse

DeBoer, Jason A.; Webber, Christa M.; Dixon, Taylor A.; Pope, Kevin L.

2015-01-01

Reservoirs can be dynamic systems, often prone to unpredictable and extreme water-level fluctuations, and can be environments where survival is difficult for zooplankton and larval fish. Although numerous studies have examined the effects of extreme reservoir drawdown on water quality, few have examined extreme drawdown on both abiotic and biotic characteristics. A fissure in the dam at Red Willow Reservoir in southwest Nebraska necessitated an extreme drawdown; the water level was lowered more than 6 m during a two-month period, reducing reservoir volume by 76%. During the subsequent low-water period (i.e., post-drawdown), spring sampling (April–June) showed dissolved oxygen concentration was lower, while turbidity and chlorophyll-a concentration were greater, relative to pre-drawdown conditions. Additionally, there was an overall increase in zooplankton density, although there were differences among taxa, and changes in mean size among taxa, relative to pre-drawdown conditions. Zooplankton assemblage composition had an average dissimilarity of 19.3% from pre-drawdown to post-drawdown. The ratio of zero to non-zero catches was greater post-drawdown for larval common carp and for all larval fishes combined, whereas we observed no difference for larval gizzard shad. Larval fish assemblage composition had an average dissimilarity of 39.7% from pre-drawdown to post-drawdown. Given the likelihood that other dams will need repair or replacement in the near future, it is imperative for effective reservoir management that we anticipate the likely abiotic and biotic responses of reservoir ecosystems as these management actions will continue to alter environmental conditions in reservoirs.

The influence of a severe reservoir drawdown on springtime zooplankton and larval fish assemblages in Red Willow Reservoir, Nebraska

USGS Publications Warehouse

DeBoer, Jason A.; Webber, Christa M.; Dixon, Taylor A.; Pope, Kevin L.

2016-01-01

Reservoirs can be dynamic systems, often prone to unpredictable and extreme water-level fluctuations, and can be environments where survival is difficult for zooplankton and larval fish. Although numerous studies have examined the effects of extreme reservoir drawdown on water quality, few have examined extreme drawdown on both abiotic and biotic characteristics. A fissure in the dam at Red Willow Reservoir in southwest Nebraska necessitated an extreme drawdown; the water level was lowered more than 6 m during a two-month period, reducing reservoir volume by 76%. During the subsequent low-water period (i.e., post-drawdown), spring sampling (April–June) showed dissolved oxygen concentration was lower, while turbidity and chlorophyll-a concentration were greater, relative to pre-drawdown conditions. Additionally, there was an overall increase in zooplankton density, although there were differences among taxa, and changes in mean size among taxa, relative to pre-drawdown conditions. Zooplankton assemblage composition had an average dissimilarity of 19.3% from pre-drawdown to post-drawdown. The ratio of zero to non-zero catches was greater post-drawdown for larval common carp and for all larval fishes combined, whereas we observed no difference for larval gizzard shad. Larval fish assemblage composition had an average dissimilarity of 39.7% from pre-drawdown to post-drawdown. Given the likelihood that other dams will need repair or replacement in the near future, it is imperative for effective reservoir management that we anticipate the likely abiotic and biotic responses of reservoir ecosystems as these management actions will continue to alter environmental conditions in reservoirs.

Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm)

PubMed Central

Echigo, Akinobu; Hino, Miki; Fukushima, Tadamasa; Mizuki, Toru; Kamekura, Masahiro; Usami, Ron

2005-01-01

Background Generally, extremophiles have been deemed to survive in the extreme environments to which they had adapted to grow. Recently many extremophiles have been isolated from places where they are not expected to grow. Alkaliphilic microorganisms have been isolated from acidic soil samples with pH 4.0, and thermophiles have been isolated from samples of low temperature. Numerous moderately halophilic microorganisms, defined as those that grow optimally in media containing 0.5–2.5 Molar (3–15%) NaCl, and halotolerant microorganisms that are able to grow in media without added NaCl and in the presence of high NaCl have been isolated from saline environments such as salterns, salt lakes and sea sands. It has tacitly been believed that habitats of halophiles able to grow in media containing more than 20% (3.4 M) are restricted to saline environments, and no reports have been published on the isolation of halophiles from ordinary garden soil samples. Results We demonstrated that many halophilic bacteria that are able to grow in the presence of 20% NaCl are inhabiting in non-saline environments such as ordinary garden soils, yards, fields and roadways in an area surrounding Tokyo, Japan. Analyses of partial 16S rRNA gene sequences of 176 isolates suggested that they were halophiles belonging to genera of the family Bacillaceae, Bacillus (11 isolates), Filobacillus (19 isolates), Gracilibacillus (6 isolates), Halobacillus (102 isolates), Lentibacillus (1 isolate), Paraliobacillus (5 isolates) and Virgibacillus (17 isolates). Sequences of 15 isolates showed similarities less than 92%, suggesting that they may represent novel taxa within the family Bacillaceae. Conclusion The numbers of total bacteria of inland soil samples were in a range from 1.4 × 107/g to 1.1 × 106/g. One tenth of the total bacteria was occupied by endospore-forming bacteria. Only very few of the endospore-forming bacteria, roughly 1 out of 20,000, are halophilic bacteria. Most of the halophilic bacteria were surviving as endospores in the soil samples, in a range of less than 1 to about 500/g soil. Samples collected from seashore in a city confronting Tokyo Bay gave the total numbers of bacteria and endospores roughly 1000 time smaller than those of inland soil samples. Numbers of halophilic bacteria per gram, however, were almost the same as those of inland soil samples. A possible source of the halophilic endospore originating from Asian dust storms is discussed. PMID:16242015

Silicon-Carbide Power MOSFET Performance in High Efficiency Boost Power Processing Unit for Extreme Environments

NASA Technical Reports Server (NTRS)

Ikpe, Stanley A.; Lauenstein, Jean-Marie; Carr, Gregory A.; Hunter, Don; Ludwig, Lawrence L.; Wood, William; Del Castillo, Linda Y.; Fitzpatrick, Fred; Chen, Yuan

2016-01-01

Silicon-Carbide device technology has generated much interest in recent years. With superior thermal performance, power ratings and potential switching frequencies over its Silicon counterpart, Silicon-Carbide offers a greater possibility for high powered switching applications in extreme environment. In particular, Silicon-Carbide Metal-Oxide- Semiconductor Field-Effect Transistors' (MOSFETs) maturing process technology has produced a plethora of commercially available power dense, low on-state resistance devices capable of switching at high frequencies. A novel hard-switched power processing unit (PPU) is implemented utilizing Silicon-Carbide power devices. Accelerated life data is captured and assessed in conjunction with a damage accumulation model of gate oxide and drain-source junction lifetime to evaluate potential system performance at high temperature environments.

Generalist feeding strategies in Arctic freshwater fish: A mechanism for dealing with extreme environments

USGS Publications Warehouse

Laske, Sarah M.; Rosenberger, Amanda E.; Wipfli, Mark S.; Zimmerman, Christian E.

2018-01-01

Generalist feeding strategies are favoured in stressful or variable environments where flexibility in ecological traits is beneficial. Species that feed across multiple habitat types and trophic levels may impart stability on food webs through the use of readily available, alternative energy pools. In lakes, generalist fish species may take advantage of spatially and temporally variable prey by consuming both benthic and pelagic prey to meet their energy demands. Using stomach content and stable isotope analyses, we examined the feeding habits of fish species in Alaska's Arctic Coastal Plain (ACP) lakes to determine the prevalence of generalist feeding strategies as a mechanism for persistence in extreme environments (e.g. low productivity, extreme cold and short growing season). Generalist and flexible feeding strategies were evident in five common fish species. Fish fed on benthic and pelagic (or nektonic) prey and across trophic levels. Three species were clearly omnivorous, feeding on fish and their shared invertebrate prey. Dietary differences based on stomach content analysis often exceeded 70%, and overlap in dietary niches based on shared isotopic space varied from zero to 40%. Metrics of community‐wide trophic structure varied with the number and identity of species involved and on the dietary overlap and niche size of individual fishes. Accumulation of energy from shared carbon sources by Arctic fishes creates redundancy in food webs, increasing likely resistance to perturbations or stochastic events. Therefore, the generalist and omnivorous feeding strategies employed by ACP fish may maintain energy flow and food web stability in extreme environments.

EFFECTS OF ULTRAVIOLET RADIATION ON THE MODERATE HALOPHILE HALOMONAS ELONGATA AND THE EXTREME HALOPHILE HALOBACTERIUM SALINARUM

EPA Science Inventory

Both the moderately halophilic bacterium, Halomonas elongata, and the extremely halophilic archaea, Halobacterium salinarum, can be found in hypersaline environments (e.g., salterns). On complex media, H. elongata grows over a salt range of 0.05-5.2 M, whereas, H. salinarum multi...

On the identification of Dragon Kings among extreme-valued outliers

NASA Astrophysics Data System (ADS)

Riva, M.; Neuman, S. P.; Guadagnini, A.

2013-07-01

Extreme values of earth, environmental, ecological, physical, biological, financial and other variables often form outliers to heavy tails of empirical frequency distributions. Quite commonly such tails are approximated by stretched exponential, log-normal or power functions. Recently there has been an interest in distinguishing between extreme-valued outliers that belong to the parent population of most data in a sample and those that do not. The first type, called Gray Swans by Nassim Nicholas Taleb (often confused in the literature with Taleb's totally unknowable Black Swans), is drawn from a known distribution of the tails which can thus be extrapolated beyond the range of sampled values. However, the magnitudes and/or space-time locations of unsampled Gray Swans cannot be foretold. The second type of extreme-valued outliers, termed Dragon Kings by Didier Sornette, may in his view be sometimes predicted based on how other data in the sample behave. This intriguing prospect has recently motivated some authors to propose statistical tests capable of identifying Dragon Kings in a given random sample. Here we apply three such tests to log air permeability data measured on the faces of a Berea sandstone block and to synthetic data generated in a manner statistically consistent with these measurements. We interpret the measurements to be, and generate synthetic data that are, samples from α-stable sub-Gaussian random fields subordinated to truncated fractional Gaussian noise (tfGn). All these data have frequency distributions characterized by power-law tails with extreme-valued outliers about the tail edges.

Perception, Action, and Cognition of Football Referees in Extreme Temperatures: Impact on Decision Performance.

PubMed

Gaoua, Nadia; de Oliveira, Rita F; Hunter, Steve

2017-01-01

Different professional domains require high levels of physical performance alongside fast and accurate decision-making. Construction workers, police officers, firefighters, elite sports men and women, the military and emergency medical professionals are often exposed to hostile environments with limited options for behavioral coping strategies. In this (mini) review we use football refereeing as an example to discuss the combined effect of intense physical activity and extreme temperatures on decision-making and suggest an explicative model. In professional football competitions can be played in temperatures ranging from -5°C in Norway to 30°C in Spain for example. Despite these conditions, the referee's responsibility is to consistently apply the laws fairly and uniformly, and to ensure the rules are followed without waning or adversely influencing the competitiveness of the play. However, strenuous exercise in extreme environments imposes increased physiological and psychological stress that can affect decision-making. Therefore, the physical exertion required to follow the game and the thermal strain from the extreme temperatures may hinder the ability of referees to make fast and accurate decisions. Here, we review literature on the physical and cognitive requirements of football refereeing and how extreme temperatures may affect referees' decisions. Research suggests that both hot and cold environments have a negative impact on decision-making but data specific to decision-making is still lacking. A theoretical model of decision-making under the constraint of intense physical activity and thermal stress is suggested. Future naturalistic studies are needed to validate this model and provide clear recommendations for mitigating strategies.

Perception, Action, and Cognition of Football Referees in Extreme Temperatures: Impact on Decision Performance

PubMed Central

Gaoua, Nadia; de Oliveira, Rita F.; Hunter, Steve

2017-01-01

Different professional domains require high levels of physical performance alongside fast and accurate decision-making. Construction workers, police officers, firefighters, elite sports men and women, the military and emergency medical professionals are often exposed to hostile environments with limited options for behavioral coping strategies. In this (mini) review we use football refereeing as an example to discuss the combined effect of intense physical activity and extreme temperatures on decision-making and suggest an explicative model. In professional football competitions can be played in temperatures ranging from -5°C in Norway to 30°C in Spain for example. Despite these conditions, the referee’s responsibility is to consistently apply the laws fairly and uniformly, and to ensure the rules are followed without waning or adversely influencing the competitiveness of the play. However, strenuous exercise in extreme environments imposes increased physiological and psychological stress that can affect decision-making. Therefore, the physical exertion required to follow the game and the thermal strain from the extreme temperatures may hinder the ability of referees to make fast and accurate decisions. Here, we review literature on the physical and cognitive requirements of football refereeing and how extreme temperatures may affect referees’ decisions. Research suggests that both hot and cold environments have a negative impact on decision-making but data specific to decision-making is still lacking. A theoretical model of decision-making under the constraint of intense physical activity and thermal stress is suggested. Future naturalistic studies are needed to validate this model and provide clear recommendations for mitigating strategies. PMID:28912742

Applying systems biology methods to the study of human physiology in extreme environments

PubMed Central

2013-01-01

Systems biology is defined in this review as ‘an iterative process of computational model building and experimental model revision with the aim of understanding or simulating complex biological systems’. We propose that, in practice, systems biology rests on three pillars: computation, the omics disciplines and repeated experimental perturbation of the system of interest. The number of ethical and physiologically relevant perturbations that can be used in experiments on healthy humans is extremely limited and principally comprises exercise, nutrition, infusions (e.g. Intralipid), some drugs and altered environment. Thus, we argue that systems biology and environmental physiology are natural symbionts for those interested in a system-level understanding of human biology. However, despite excellent progress in high-altitude genetics and several proteomics studies, systems biology research into human adaptation to extreme environments is in its infancy. A brief description and overview of systems biology in its current guise is given, followed by a mini review of computational methods used for modelling biological systems. Special attention is given to high-altitude research, metabolic network reconstruction and constraint-based modelling. PMID:23849719

LiDAR in extreme environment: surveying in Antarctica

NASA Astrophysics Data System (ADS)

Abate, D.; Pierattini, S.; Bianchi Fasani, G.

2013-10-01

This study was performed under the patronage of the Italian National Research Programme in Antarctica (PNRA) with the aim to realize a high resolution Digital Elevation Model (DEM) of the moraine named "Boulder Clay" which insists approximately 7 km far from the Italian Research Base "Mario Zucchelli Station" in the Terra Nova Bay area. The DEM will be included in the project for the construction of two runways to be used as support facilities for the scientific research campaigns which take place on regular basis each year. Although the research efforts to realize a detailed cartography of the area is on-going, for the specific aim and urgency of this project it was decided to perform a laser scanning survey in this extreme environment in order to obtain contour lines describing the terrain elevation each 50 cm and volume analysis. The final result will be super imposed on a photogrammetric DEM with contour lines each 2.5 m and satellite images. This paper focus both on the final scientific data and on all the challenges have to be faced in such extreme and particular environment during the laser scanning survey.

Microorganisms in desert rocks: the edge of life on Earth.

PubMed

Wierzchos, Jacek; de los Ríos, Asunción; Ascaso, Carmen

2012-12-01

This article reviews current knowledge on microbial communities inhabiting endolithic habitats in the arid and hyper-arid regions of our planet. In these extremely dry environments, the most common survival strategy is to colonize the interiors of rocks. This habitat provides thermal buffering, physical stability, and protection against incident UV radiation, excessive photosynthetically active radiation, and freeze-thaw events. Above all, through water retention in the rocks' network of pores and fissures, moisture is made available. Some authors have argued that dry environments pose the most extreme set of conditions faced by microorganisms. Microbial cells need to withstand the biochemical stresses created by the lack of water, along with temperature fluctuations and/or high salinity. In this review, we also address the variety of ways in which microorganisms deal with the lack of moisture in hyper-arid environments and point out the diversity of microorganisms that are able to cope with only the scarcest presence of water. Finally, we discuss the important clues to the history of life on Earth, and perhaps other places in our solar system, that have emerged from the study of extreme microbial ecosystems.

A New Time-varying Concept of Risk in a Changing Climate.

PubMed

Sarhadi, Ali; Ausín, María Concepción; Wiper, Michael P

2016-10-20

In a changing climate arising from anthropogenic global warming, the nature of extreme climatic events is changing over time. Existing analytical stationary-based risk methods, however, assume multi-dimensional extreme climate phenomena will not significantly vary over time. To strengthen the reliability of infrastructure designs and the management of water systems in the changing environment, multidimensional stationary risk studies should be replaced with a new adaptive perspective. The results of a comparison indicate that current multi-dimensional stationary risk frameworks are no longer applicable to projecting the changing behaviour of multi-dimensional extreme climate processes. Using static stationary-based multivariate risk methods may lead to undesirable consequences in designing water system infrastructures. The static stationary concept should be replaced with a flexible multi-dimensional time-varying risk framework. The present study introduces a new multi-dimensional time-varying risk concept to be incorporated in updating infrastructure design strategies under changing environments arising from human-induced climate change. The proposed generalized time-varying risk concept can be applied for all stochastic multi-dimensional systems that are under the influence of changing environments.

Investigation of Loop Heat Pipe Survival and Restart After Extreme Cold Environment Exposure

NASA Technical Reports Server (NTRS)

Golliher, Eric; Ku, Jentung; Licari, Anthony; Sanzi, James

2010-01-01

NASA plans human exploration near the South Pole of the Moon, and other locations where the environment is extremely cold. This paper reports on the heat transfer performance of a loop heat pipe (LHP) exposed to extreme cold under the simulated reduced gravitational environment of the Moon. A common method of spacecraft thermal control is to use a LHP with ammonia working fluid. Typically, a small amount of heat is provided either by electrical heaters or by environmental design, such that the LHP condenser temperature never drops below the freezing point of ammonia. The concern is that a liquid-filled, frozen condenser would not restart, or that a thawing condenser would damage the tubing due to the expansion of ammonia upon thawing. This paper reports the results of an experimental investigation of a novel approach to avoid these problems. The LHP compensation chamber (CC) is conditioned such that all the ammonia liquid is removed from the condenser and the LHP is nonoperating. The condenser temperature is then reduced to below that of the ammonia freezing point. The LHP is then successfully restarted.

Current thinking about acute compartment syndrome of the lower extremity

PubMed Central

Shadgan, Babak; Menon, Matthew; Sanders, David; Berry, Gregg; Martin, Claude; Duffy, Paul; Stephen, David; O’Brien, Peter J.

2010-01-01

Acute compartment syndrome of the lower extremity is a clinical condition that, although uncommon, is seen fairly regularly in modern orthopedic practice. The pathophysiology of the disorder has been extensively described and is well known to physicians who care for patients with musculoskeletal injuries. The diagnosis, however, is often difficult to make. In this article, we review the clinical risk factors of acute compartment syndrome of the lower extremity, identify the current concepts of diagnosis and discuss appropriate treatment plans. We also describe the Canadian medicolegal environment in regard to compartment syndrome of the lower extremity. PMID:20858378

Impact of the extreme 2009 wildfire Victoria the wettability of naturally highly water repellent soils

NASA Astrophysics Data System (ADS)

Doerr, Stefan H.; Shakesby, Richard A.; Sheridan, Gary J.; Lane, Patrick Nj; Smith, Hugh G.; Bell, Tina; Blake, William H.

2010-05-01

The recent catastrophic wildfires near Melbourne, which peaked on Feb. 7 2009, burned ca 400,000 ha and caused the tragic loss of 173 people. They occurred during unprecedented extreme fire weather where dry northerly winds gusting up to 100 km/h coincided with the highest temperatures ever recorded in this region. These conditions, combined with the very high biomass of mature eucalypt forests, very low fuel moisture conditions and steep slopes, generated extreme burning conditions. A rapid response project was launched under the NERC Urgency Scheme aimed at determining the effects of this extreme event on soil properties. Three replicate sites each were sampled for extremely high burn severity, high burn severity and unburnt control terrain, within mature mixed-species eucalypt forests near Marysville in April 2009. Ash and surface soil (0-2.5 cm and 2.5-5 cm) were collected at 20 sample grid points at each site. Here we report on outcomes from Water Drop Penetration Time (WDPT) tests carried out on soil samples to determine the impact of this extreme event on the wettability of a naturally highly water repellent soil. Field assessment suggested that the impact of this extreme wildfire on the soil was less than might be supposed given the extreme burn severity (indicated by the complete elimination of the ground vegetation). This was confirmed by the laboratory results. No major difference in WDPT was detected between (i) burned and control samples, and (ii) between surface and subsurface WDPT patterns, indicating that soil temperatures in the top 0-2.5 cm did not exceed ~200° C. Seedling germination in burned soil was reduced by at least 2/3 compared to the control samples, however, this reduction is indicative an only modest heat input into the soil. The limited heat input into the soil stands in stark contrast to the extreme burn severity (based on vegetation destruction parameters). We speculate that limited soil heating resulted perhaps from the unusually fast-moving fire front and the resultant short fire residence time during this event. Thick ash layers were present at the time of sampling despite some significant earlier pre-sampling rainfall events. This suggests that the wettable ash (up to 15 cm thick) was able to store substantial amounts of water, which would otherwise have formed overland flow moving over the highly water repellent underlying mineral soil. Once this hydrological ‘sponge' is removed, the lack of ground cover is expected to lead to the underlying soil being susceptible to erosion until the ground cover becomes re-established. This ‘erosion window‘ is likely to be relatively brief over much of the burnt area as the vegetation is already showing a comparatively rapid regrowth response. This is supported by initial results from laboratory germination experiments, which showed seedling emergence from even the most severely burnt sites. The factors contributing to the fire impacts determined here are explored in conjunction with predictions for future burn severity under a changing climate. The soil samples collected represent a reference soil sample collection, which are available to the scientific community for further investigation.

ARL Collaborative Research Alliance Materials in Extreme Dynamic Environments (MEDE)

DTIC Science & Technology

2010-11-19

Program Internal to the CRA Staff Rotation Lectures, Workshops, and Research Reviews Education Opportunities for Government Personnel Student ... Engagement with ARL Research Environment Industry Partnership + Collaboration Other Collaboration Opportunities High Performance Computing DoD

Biosignatures of Hypersaline Environments (Salt Crusts) an Analog for Mars

NASA Astrophysics Data System (ADS)

Smith, H. D.; Duncan, A. G.; Davilla, A. F.; McKay, C. P.

2016-05-01

Halophilic ecosystems are models for life in extreme environments including planetary surfaces such as Mars. Our research focuses on biosignatures in a salt crusts and the detection of these biomarkers by ground and orbital assests.

Transport Functions Dominate the SAR11 Metaproteome at Low-Nutrient Extremes in the Sargasso Sea

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sowell, Sarah M.; Wilhelm, Larry; Norbeck, Angela D.

2009-01-01

The northwestern Sargasso Sea is part of the North Atlantic subtropical oceanic gyre that is characterized as seasonally oligotrophic with pronounced stratification in the summer and autumn. Essentially a marine desert, the biological productivity of this region is reduced during stratified periods as a result of low concentrations of phosphorous and nitrogen in the euphotic zone. To better understand the mechanisms of microbial survival in this oligotrophic environment, we used capillary LC-tandem mass spectrometry to study the composition of microbial proteomes in surface samples collected in September 2005. A total of 2279 peptides that mapped to 236 SAR11 proteins, andmore » 3208 peptides that mapped to 404 Synechococcus proteins, were detected. Mass spectra from SAR11 periplasmic binding proteins accounted for a disproportionately large fraction of the peptides detected, consistent with observations that these extremely small cells devote a large proportion of their volume to periplasm. Abundances were highest for periplasmic substrate-binding proteins for phosphate, amino acids, phosphonate, sugars, and spermidine. Although the data showed that a large fraction of microbial protein synthesis in the Sargasso Sea is devoted to inorganic and organic nutrient acquisition, the proteomes of both SAR11 and Synechococcus also indicated that these populations were actively growing. Our findings support the view that competition for multiple nutrients in oligotrophic systems is extreme but sufficient to sustain microbial community activity.« less

Toxicity at the Edge of Life: A Review on Cyanobacterial Toxins from Extreme Environments

PubMed Central

Casero, María Cristina

2017-01-01

Cyanotoxins are secondary metabolites produced by cyanobacteria, of varied chemical nature and toxic effects. Although cyanobacteria thrive in all kinds of ecosystems on Earth even under very harsh conditions, current knowledge on cyanotoxin distribution is almost restricted to freshwaters from temperate latitudes. In this review, we bring to the forefront the presence of cyanotoxins in extreme environments. Cyanotoxins have been reported especially in polar deserts (both from the Arctic and Antarctica) and alkaline lakes, but also in hot deserts, hypersaline environments, and hot springs. Cyanotoxins detected in these ecosystems include neurotoxins—anatoxin-a, anatoxin-a (S), paralytic shellfish toxins, β-methylaminopropionic acid, N-(2-aminoethyl) glycine and 2,4-diaminobutyric acid- and hepatotoxins –cylindrospermopsins, microcystins and nodularins—with microcystins being the most frequently reported. Toxin production there has been linked to at least eleven cyanobacterial genera yet only three of these (Arthrospira, Synechococcus and Oscillatoria) have been confirmed as producers in culture. Beyond a comprehensive analysis of cyanotoxin presence in each of the extreme environments, this review also identifies the main knowledge gaps to overcome (e.g., scarcity of isolates and –omics data, among others) toward an initial assessment of ecological and human health risks in these amazing ecosystems developing at the very edge of life. PMID:28737704

Toxicity at the Edge of Life: A Review on Cyanobacterial Toxins from Extreme Environments.

PubMed

Cirés, Samuel; Casero, María Cristina; Quesada, Antonio

2017-07-24

Cyanotoxins are secondary metabolites produced by cyanobacteria, of varied chemical nature and toxic effects. Although cyanobacteria thrive in all kinds of ecosystems on Earth even under very harsh conditions, current knowledge on cyanotoxin distribution is almost restricted to freshwaters from temperate latitudes. In this review, we bring to the forefront the presence of cyanotoxins in extreme environments. Cyanotoxins have been reported especially in polar deserts (both from the Arctic and Antarctica) and alkaline lakes, but also in hot deserts, hypersaline environments, and hot springs. Cyanotoxins detected in these ecosystems include neurotoxins-anatoxin-a, anatoxin-a (S), paralytic shellfish toxins, β-methylaminopropionic acid, N -(2-aminoethyl) glycine and 2,4-diaminobutyric acid- and hepatotoxins -cylindrospermopsins, microcystins and nodularins-with microcystins being the most frequently reported. Toxin production there has been linked to at least eleven cyanobacterial genera yet only three of these ( Arthrospira , Synechococcus and Oscillatoria ) have been confirmed as producers in culture. Beyond a comprehensive analysis of cyanotoxin presence in each of the extreme environments, this review also identifies the main knowledge gaps to overcome (e.g., scarcity of isolates and -omics data, among others) toward an initial assessment of ecological and human health risks in these amazing ecosystems developing at the very edge of life.

The Impact of Assembly Bias on the Galaxy Content of Dark Matter Halos

NASA Astrophysics Data System (ADS)

Zehavi, Idit; Contreras, Sergio; Padilla, Nelson; Smith, Nicholas J.; Baugh, Carlton M.; Norberg, Peder

2018-01-01

We study the dependence of the galaxy content of dark matter halos on large-scale environment and halo formation time using semi-analytic galaxy models applied to the Millennium simulation. We analyze subsamples of halos at the extremes of these distributions and measure the occupation functions for the galaxies they host. We find distinct differences among these occupation functions. The main effect with environment is that central galaxies (and in one model, also the satellites) in denser regions start populating lower-mass halos. A similar, but significantly stronger, trend exists with halo age, where early-forming halos are more likely to host central galaxies at lower halo mass. We discuss the origin of these trends and the connection to the stellar mass–halo mass relation. We find that, at fixed halo mass, older halos and to some extent also halos in dense environments tend to host more massive galaxies. Additionally, we see a reverse trend for the occupation of satellite galaxies where early-forming halos have fewer satellites, likely due to having more time for them to merge with the central galaxy. We describe these occupancy variations in terms of the changes in the occupation function parameters, which can aid in constructing realistic mock galaxy samples. Finally, we study the corresponding galaxy auto- and cross-correlation functions of the different samples and elucidate the impact of assembly bias on galaxy clustering. Our results can inform theoretical modeling of galaxy assembly bias and attempts to detect it in the real universe.

Lack of Microbial Diversity in an Extreme Mars Analog Setting: Poás Volcano, Costa Rica.

PubMed

Hynek, Brian M; Rogers, Karyn L; Antunovich, Monique; Avard, Geoffroy; Alvarado, Guillermo E

2018-04-24

The Poás volcano in Costa Rica has been studied as a Mars geochemical analog environment, since both the style of hydrothermal alteration present and the alteration mineralogy are consistent with Mars' relict hydrothermal systems. The site hosts an active volcano, with high-temperature fumaroles (up to 980°C) and an ultra-acidic lake. This lake, Laguna Caliente, is one of the most dynamic environments on Earth, with frequent phreatic eruptions, temperatures ranging from near-ambient to almost boiling, a pH range of -1 to 1.5, and a wide range of chemistries and redox potential. Martian acid-sulfate hydrothermal systems were likely similarly dynamic and equally challenging to life. The microbiology existing within Laguna Caliente was characterized for the first time, with sampling taking place in November, 2013. The diversity of the microbial community was surveyed via extraction of environmental DNA from fluid and sediment samples followed by Illumina sequencing of the 16S rRNA gene. The microbial diversity was limited to a single species of the bacterial genus Acidiphilium. This organism likely gets its energy from oxidation of reduced sulfur in the lake, including elemental sulfur. Given Mars' propensity for sulfur and acid-sulfate environments, this type of organism is of significant interest to the search for past or present life on the Red Planet. Key Words: Mars astrobiology-Acid-sulfate hydrothermal systems-Extremophiles-Acidic-High temperature-Acidiphilium bacteria. Astrobiology 18, xxx-xxx.

Clumped Isotope Values from the Doushantuo Formation of South China: Evaluation of Hydrothermal Influence, Disequilibria and Diagenetic Effects

NASA Astrophysics Data System (ADS)

Foster, I. S.; Zhu, M.; Lu, M.; Bristow, T.; Bonifacie, M.; Tripati, A.

2015-12-01

The Ediacaran Doushantuo Formation (635 - 551 Ma) of southern China is a phosphate-dolostone-black shale sequence following the Marinoan "Snowball Earth" episode that represents an important period in Earth history. It contains abundant phosphate-preserved microfossils, and extremely low carbon isotope values in the cap dolostone unit that have been interpreted to reflect formation in a methane seep environment [1]. Previous clumped isotope analysis of 13C-depleted carbonate veins from the basal Doushantuo samples have been interpreted to reflect hydrothermally-derived thermogenic methane oxidation [2], however recent work on modern and ancient cold seep samples suggests clumped isotope signatures in these environments are influenced by disequilibria effects [3] and are vulnerable to post-depositional diagenesis via internal reordering at temperatures exceeding ~ 100 - 120 ˚C [4]. Here we present initial data from the cap-carbonates overlying the Nantuo diamictite. Our analysis includes a variety of micro-facies from the cap-carbonate including pure calcite and micrite, with a range of carbonate carbon isotopic values. Data presented here will be used to attempt to determine if the samples exhibit disequilibria effects such as those observed in modern cold seep environments, as well as to evaluate the role of hydrothermal activity in the Doushantuo Formation. [1] Jiang, G., Kennedy, M.J., Christie-Blick, N., 2003. Stable isotope evidence for methane seeps in Neoproterozoic postglacial cap carbonates. Nature 426, 822-826. [2] Bristow, T.F., Bonifacie, M., Derkowski, A., Eiler, J.M., Grotzinger, J.P., 2011. A hydrothermal origin for isotopically anomalous cap dolostone cements from south China. Nature 474, 68-72. [3] Loyd, S., Sample, J.C., Orphan, V.J., Marlow, J., Eagle, R., Tripati, A.K., 2012. Clumped isotope analyses of cold seep carbonates: Insights into formation environment and mechanisms. Abstract B51G-0639 presented at 2012 Fall Meeting, AGU, San Francisco, Calif., 3-7 Dec. [4] Henkes, G.A., Passey, B.H., Grossman, E.L., Shenton, B.J., Pérez-Huerta, A., Yancey, E.L., 2014. Temperature limits for preservation of primary calcite clumped isotope paleotemperatures. Geochemica et Cosmochimica Acta 139, 362-382.

Three Essays on the Role of Information and Communication Technologies in Organizations

ERIC Educational Resources Information Center

Ada, Serkan

2011-01-01

Information and communication technologies (ICT) have an essential role in today's organizations and ever-changing dynamic environments. ICT has substantial tangible and intangible impact on organizations not only in usual environments, but also in extreme environments. This dissertation is composed of three essays on the impact of ICT in…

Pediatric Cystic Fibrosis Sputum Can Be Chemically Dynamic, Anoxic, and Extremely Reduced Due to Hydrogen Sulfide Formation

PubMed Central

Cowley, Elise S.; Kopf, Sebastian H.; LaRiviere, Alejandro

2015-01-01

ABSTRACT Severe and persistent bacterial lung infections characterize cystic fibrosis (CF). While several studies have documented the microbial diversity within CF lung mucus, we know much less about the inorganic chemistry that constrains microbial metabolic processes and their distribution. We hypothesized that sputum is chemically heterogeneous both within and between patients. To test this, we measured microprofiles of oxygen and sulfide concentrations as well as pH and oxidation-reduction potentials in 48 sputum samples from 22 pediatric patients with CF. Inorganic ions were measured in 20 samples from 12 patients. In all cases, oxygen was depleted within the first few millimeters below the sputum-air interface. Apart from this steep oxycline, anoxia dominated the sputum environment. Different sputum samples exhibited a broad range of redox conditions, with either oxidizing (16 mV to 355 mV) or reducing (−300 to −107 mV) potentials. The majority of reduced samples contained hydrogen sulfide and had a low pH (2.9 to 6.5). Sulfide concentrations increased at a rate of 0.30 µM H2S/min. Nitrous oxide was detected in only one sample that also contained sulfide. Microenvironmental variability was observed both within a single patient over time and between patients. Modeling oxygen dynamics within CF mucus plugs indicates that anoxic zones vary as a function of bacterial load and mucus thickness and can occupy a significant portion of the mucus volume. Thus, aerobic respiration accounts only partially for pathogen survival in CF sputum, motivating research to identify mechanisms of survival under conditions that span fluctuating redox states, including sulfidic environments. PMID:26220964

Quantitative habitability.

PubMed

Shock, Everett L; Holland, Melanie E

2007-12-01

A framework is proposed for a quantitative approach to studying habitability. Considerations of environmental supply and organismal demand of energy lead to the conclusions that power units are most appropriate and that the units for habitability become watts per organism. Extreme and plush environments are revealed to be on a habitability continuum, and extreme environments can be quantified as those where power supply only barely exceeds demand. Strategies for laboratory and field experiments are outlined that would quantify power supplies, power demands, and habitability. An example involving a comparison of various metabolisms pursued by halophiles is shown to be well on the way to a quantitative habitability analysis.

Assessments and Viewpoints on the Biological and Human Health Effects of Extremely Low Frequency (ELF) Electromagnetic Fields. Compilation of Commissioned Papers for the ELF Literature Review Project.

DTIC Science & Technology

1985-05-01

Environ. Biophys. 20:53-65. 1983. Electric field effects on bacteria and yeast cells . Radiat. Environ. Biophys. 22 :149-162. Husing, J. 0., F. Strauss, and...Jr., Ph.D. 141 A Review of Cell Effects Induced by Exposure of Extremely Low 155 Frequency Electromagnetic Fields - Eugene M. Goodman, Ph.D. and Ben...and E. M. Goodman. 1983. Cell surface effects of 60 Hz electromagnetic fields. Radiat. Res. 94:217-220. artucci, G. I., P. C. Gailey, and R. A. Tell

Electric motor-transformer aggregate in hermetic objects of transport vehicles

NASA Astrophysics Data System (ADS)

Zabora, Igor

2017-10-01

The construction and features of operation for new electrical unit - electric motor-transformer aggregate (DTA) are considered. Induction motors are intended for operation in hermetic plants with extreme conditions surrounding gas, steam-to-gas and liquid environment at a high temperature (to several hundred of degrees). Main objective of spent researches is the substantiation of possibility reliable and effective electric power transform with electric machine means directly in hermetic objects with extreme conditions environment by means of new DTA. The principle and job analysis of new disk induction motors of block-module type are observed.

Communication path for extreme environments

NASA Technical Reports Server (NTRS)

Jorgensen, Charles C. (Inventor); Betts, Bradley J. (Inventor)

2010-01-01

Methods and systems for using one or more radio frequency identification devices (RFIDs), or other suitable signal transmitters and/or receivers, to provide a sensor information communication path, to provide location and/or spatial orientation information for an emergency service worker (ESW), to provide an ESW escape route, to indicate a direction from an ESW to an ES appliance, to provide updated information on a region or structure that presents an extreme environment (fire, hazardous fluid leak, underwater, nuclear, etc.) in which an ESW works, and to provide accumulated thermal load or thermal breakdown information on one or more locations in the region.

Radiation Hardened Electronics for Extreme Environments

NASA Technical Reports Server (NTRS)

Keys, Andrew S.; Watson, Michael D.

2007-01-01

The Radiation Hardened Electronics for Space Environments (RHESE) project consists of a series of tasks designed to develop and mature a broad spectrum of radiation hardened and low temperature electronics technologies. Three approaches are being taken to address radiation hardening: improved material hardness, design techniques to improve radiation tolerance, and software methods to improve radiation tolerance. Within these approaches various technology products are being addressed including Field Programmable Gate Arrays (FPGA), Field Programmable Analog Arrays (FPAA), MEMS Serial Processors, Reconfigurable Processors, and Parallel Processors. In addition to radiation hardening, low temperature extremes are addressed with a focus on material and design approaches.

Terrestrial environment (climatic) criteria guidelines for use in aerospace vehicle development

NASA Technical Reports Server (NTRS)

Turner, R. E. (Compiler); Hill, C. K. (Compiler)

1982-01-01

Guidelines on terrestrial environment data specifically applicable for NASA aerospace vehicles and associated equipment development are provided. The general distribution of natural environmental extremes in the conterminous United States that may be needed to specify design criteria in the transportation of space vehicle subsystems and components is considered. Atmospheric attenuation was included, since certain Earth orbital experiment missions are influenced by the Earth's atmosphere. Climatic extremes for worldwide operational needs is also included. Atmospheric chemistry, seismic criteria, and a mathematical model to predict atmospheric dispersion of aerospace engine exhaust cloud rise and growth are discussed. Atmospheric cloud phenomena are considered.

Overview of Heatshield for Extreme Entry Environment Technology (HEEET)

NASA Technical Reports Server (NTRS)

Driver, David M.; Ellerby, Donald T.; Gasch, Matthew J.; Mahzari, Milad; Milos, Frank S.; Nishioka, Owen S.; Stackpoole, Margaret M.; Venkatapathy, Ethiraj; Young, Zion W.; Gage, Peter J.;

Mercury contamination of aquatic ecosystems

USGS Publications Warehouse

Krabbenhoft, David P.; Rickert, David A.

1995-01-01

Mercury has been well known as an environmental pollutant for several decades. As early as the 1950's it was established that emissions of mercury to the environment could have serious effects on human health. These early studies demonstrated that fish and other wildlife from various ecosystems commonly attain mercury levels of toxicological concern when directly affected by mercury-containing emissions from human-related activities. Human health concerns arise when fish and wildlife from these ecosystems are consumed by humans. During the past decade, a new trend has emerged with regard to mercury pollution. Investigations initiated in the late 1980's in the northern-tier states of the U.S., Canada, and Nordic countries found that fish, mainly from nutrient-poor lakes and often in very remote areas, commonly have high levels of mercury. More recent fish sampling surveys in other regions of the U.S. have shown widespread mercury contamination in streams, wet-lands, reservoirs, and lakes. To date, 33 states have issued fish consumption advisories because of mercury contamination. These continental to global scale occurrences of mercury contamination cannot be linked to individual emissions of mercury, but instead are due to widespread air pollution. When scientists measure mercury levels in air and surface water, however, the observed levels are extraordinarily low. In fact, scientists have to take extreme precautions to avoid direct contact with water samples or sample containers, to avert sample contamination (Fig 3). Herein lies an apparent discrepancy: Why do fish from some remote areas have elevated mercury concentrations, when contamination levels in the environment are so low?

Compression Strength of Sulfur Concrete Subjected to Extreme Cold

NASA Technical Reports Server (NTRS)

Grugel, Richard N.

2008-01-01

Sulfur concrete cubes were cycled between liquid nitrogen and room temperature to simulate extreme exposure conditions. Subsequent compression testing showed the strength of cycled samples to be roughly five times less than those non-cycled. Fracture surface examination showed de-bonding of the sulfur from the aggregate material in the cycled samples but not in those non-cycled. The large discrepancy found, between the samples is attributed to the relative thermal properties of the materials constituting the concrete.

NEEMO - NASA's Extreme Environment Mission Operations: On to a NEO

NASA Technical Reports Server (NTRS)

Bell, M. S.; Baskin, P. J.; Todd, W. L.

2011-01-01

During NEEMO missions, a crew of six Aquanauts lives aboard the National Oceanic and Atmospheric Administration (NOAA) Aquarius Underwater Laboratory the world's only undersea laboratory located 5.6 km off shore from Key Largo, Florida. The Aquarius habitat is anchored 62 feet deep on Conch Reef which is a research only zone for coral reef monitoring in the Florida Keys National Marine Sanctuary. The crew lives in saturation for a week to ten days and conducts a variety of undersea EVAs (Extra Vehicular Activities) to test a suite of long-duration spaceflight Engineering, Biomedical, and Geoscience objectives. The crew also tests concepts for future lunar exploration using advanced navigation and communication equipment in support of the Constellation Program planetary exploration analog studies. The Astromaterials Research and Exploration Science (ARES) Directorate and Behavioral Health and Performance (BHP) at NASA/Johnson Space Center (JSC), Houston, Texas support this effort to produce a high-fidelity test-bed for studies of human planetary exploration in extreme environments as well as to develop and test the synergy between human and robotic curation protocols including sample collection, documentation, and sample handling. The geoscience objectives for NEEMO missions reflect the requirements for Lunar Surface Science outlined by the LEAG (Lunar Exploration Analysis Group) and CAPTEM (Curation and Analysis Planning Team for Extraterrestrial Materials) white paper [1]. The BHP objectives are to investigate best meas-ures and tools for assessing decrements in cogni-tive function due to fatigue, test the feasibility study examined how teams perform and interact across two levels, use NEEMO as a testbed for the development, deployment, and evaluation of a scheduling and planning tool. A suite of Space Life Sciences studies are accomplished as well, ranging from behavioral health and performance to immunology, nutrition, and EVA suit design results of which will directly support the investigation of open questions and operational concepts that will enable NASA to continue its plan for planetary exploration.

Radiocarbon dating and Dendrochronology for Statigraphic Units near Tebano, Senio Northern Apennines - Time frame of Climatic Fluctuation at the onset of the Younger Dryas

NASA Astrophysics Data System (ADS)

Eggenschwiler, Loren; Hajdas, Irka; Cherubini, Paolo; Picotti, Vincenzo; Saurer, Matthias

2017-04-01

The presence of Pinus [sylvestris] provides an insight into dramatic events due to climatic changes. Several major and minor climatic fluctuations have had a strong impact on terrestrial and marine environments since the last glacial period to present day (Ravazzi et al. 2006). This study aims to describe the response of a fluvial environment through the use of dendrochronology and stratigraphy. Here, we intend to get a better understanding of how these climatic fluctuations affect the behavior of the Senio River (Lotter et al. 1992). In Tebano, Italy, several Pinus sylvestris subfossil trunks were discovered during excavation for an irrigation pool. Subfossil samples were collected to analyze the climate during the Younger Dryas (11,000 years BP) in detail. Charcoal samples from the Bubano clay quarry extend our research to further to 35,500 cal. years BP. The combination of dendrochronology along with stratigraphy allowed us to examine the climate at a detailed local and apply it to a broader spectrum. Tree-ring measurements and cross dating provided a better understanding and verification of extreme events that occurred during the lifespans of the trees. The use of stable isotopes indicates the extreme conditions that occurred. Radiocarbon dating validates the age of the samples and what geological period they come from. Along with stratigraphy, we were able to compile depth data to create a sediment curve. Using various methods throughout this study, we discovered the climatic situation of Pinus 11,000 years BP and are able to compare them with samples from today. These present day samples mark two of the southernmost extents of the Pinus population. We were then able to comprehend the magnitude of sediment supply and precipitation. Through this collection of methods and data, we are able to understand the influence of climate change in the past and the potential changes of the future. REFERENCES Lotter, A. F.; Eicher, U.; Siegenthaler, U.; Birks, H. J. B. (1992): Late-glacial climatic oscillations as recorded in Swiss lake sediments. In Journal of Quaternary Science 7. DOI: 10.1002/jqs.3390070302. Ravazzi, Cesare; Donegana, Marta; Vescovi, Elisa; Arpenti, Enrico; Caccianiga, Marco; Kaltenrieder, Petra et al. (2006): A new Late-glacial site with Picea abies in the northern Apennine foothills. An exception to the model of glacial refugia of trees. In Veget Hist Archaeobot 15 (4), pp. 357-371. DOI: 10.1007/s00334-006-0055-9.

Galaxy evolution in extreme environments: Molecular gas content star formation and AGN in isolated void galaxies

NASA Astrophysics Data System (ADS)

Das, Mousumi; Iono, Daisuke; Saito, Toshiki; Subramanian, Smitha

Since the early redshift surveys of the large scale structure of our universe, it has become clear that galaxies cluster along walls, sheet and filaments leaving large, empty regions called voids between them. Although voids represent the most under dense parts of our universe, they do contain a sparse but significant population of isolated galaxies that are generally low luminosity, late type disk galaxies. Recent studies show that most void galaxies have ongoing star formation and are in an early stage of evolution. We present radio, optical studies of the molecular gas content and star formation in a sample of void galaxies. Using SDSS data, we find that AGN are rare in these systems and are found only in the Bootes void; their black hole masses and radio properties are similar to bright spirals galaxies. Our studies suggest that close galaxy interactions and gas accretion are the main drivers of galaxy evolution in these systems despite their location in the underdense environment of the voids.

Life Detection and Characterization of Subsurface Ice and Brine in the McMurdo Dry Valleys Using an Ultrasonic Gopher: A NASA ASTEP Project

NASA Technical Reports Server (NTRS)

Doran, P. T.; Bar-Cohen, Y.; Fritsen, C.; Kenig, F.; McKay, C. P.; Murray, A.; Sherrit, S.

2003-01-01

Evidence for the presence of ice and fluids near the surface of Mars in both the distant and recent past is growing with each new mission to the Planet. One explanation for fluids forming springlike features on Mars is the discharge of subsurface brines. Brines offer potential refugia for extant Martian life, and near surface ice could preserve a record of past life on the planet. Proven techniques to get underground to sample these environments, and get below the disruptive influence of the surface oxidant and radiation regime, will be critical for future astrobiology missions to Mars. Our Astrobiology for Science and Technology for Exploring Planets (ASTEP) project has the goal to develop and test a novel ultrasonic corer in a Mars analog environment, the McMurdo Dry valleys, Antarctica, and to detect and describe life in a previously unstudied extreme ecosystem; Lake Vida (Fig. 1), an ice-sealed lake.

Endemic and widespread coral reef fishes have similar mitochondrial genetic diversity.

PubMed

Delrieu-Trottin, Erwan; Maynard, Jeffrey; Planes, Serge

2014-12-22

Endemic species are frequently assumed to have lower genetic diversity than species with large distributions, even if closely related. This assumption is based on research from the terrestrial environment and theoretical evolutionary modelling. We test this assumption in the marine environment by analysing the mitochondrial genetic diversity of 33 coral reef fish species from five families sampled from Pacific Ocean archipelagos. Surprisingly, haplotype and nucleotide diversity did not differ significantly between endemic and widespread species. The probable explanation is that the effective population size of some widespread fishes locally is similar to that of many of the endemics. Connectivity across parts of the distribution of the widespread species is probably low, so widespread species can operate like endemics at the extreme or isolated parts of their range. Mitochondrial genetic diversity of many endemic reef fish species may not either limit range size or be a source of vulnerability. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

Enrichments for phototrophic bacteria and characterization by morphology and pigment analysis

NASA Technical Reports Server (NTRS)

Brune, D.

1985-01-01

The purpose of this investigation was to examine several sulfide containing environments for the presence of phototrophic bacteria and to obtain enriched cultures of some of the bacteria present. The field sites were Alum Rock State Park, the Palo Alto salt marsh, the bay area salt ponds, and Big Soda Lake (near Fallon, Nevada). Bacteria from these sites were characterized by microscopic examination, measurement of in vitro absorption spectra, and analysis of carotenoid pigments. Field observations at one of the bay area salt ponds, in which the salt concentration was saturating (about 30 percent NaCl) and the sediments along the shore of the pond covered with a gypsum crust, revealed a layer of purple photosynthetic bacteria under a green layer in the gypsum crust. Samples of this gypsum crust were taken to the laboratory to measure light transmission through the crust and to try to identify the purple photosynthetic bacteria present in this extremely saline environment.

Reliability of Sn/Pb and Lead-Free (SnAgCu) Solders of Surface Mounted Miniaturized Passive Components for Extreme Temperature (-185 C to +125 C) Space Missions

NASA Technical Reports Server (NTRS)

Ramesham, Rajeshuni

2011-01-01

Surface mount electronic package test boards have been assembled using tin/lead (Sn/Pb) and lead-free (Pb-free or SnAgCu or SAC305) solders. The soldered surface mount packages include ball grid arrays (BGA), flat packs, various sizes of passive chip components, etc. They have been optically inspected after assembly and subsequently subjected to extreme temperature thermal cycling to assess their reliability or future deep space, long-term, extreme temperature environmental missions. In this study, the employed temperature range (-185oC to +125oC) covers military specifications (-55oC to +100oC), extreme old Martian (-120oC to +115oC), asteroid Nereus (-180oC to +25oC) and JUNO (-150oC to +120oC) environments. The boards were inspected at room temperature and at various intervals as a function of extreme temperature thermal cycling and bake duration. Electrical resistance measurements made at room temperature are reported and the tests to date have shown some change in resistance as a function of extreme temperature thermal cycling and some showed increase in resistance. However, the change in interconnect resistance becomes more noticeable with increasing number of thermal cycles. Further research work will be carried out to understand the reliability of packages under extreme temperature applications (-185oC to +125oC) via continuously monitoring the daisy chain resistance for BGA, Flat-packs, lead less chip packages, etc. This paper will describe the experimental reliability results of miniaturized passive components (01005, 0201, 0402, 0603, 0805, and 1206) assembled using surface mounting processes with tin-lead and lead-free solder alloys under extreme temperature environments.

Reliability of Sn/Pb and lead-free (SnAgCu) solders of surface mounted miniaturized passive components for extreme temperature (-185°C to +125°C) space missions

NASA Astrophysics Data System (ADS)

Ramesham, Rajeshuni

2011-02-01

Surface mount electronic package test boards have been assembled using tin/lead (Sn/Pb) and lead-free (Pb-free or SnAgCu or SAC305) solders. The soldered surface mount packages include ball grid arrays (BGA), flat packs, various sizes of passive chip components, etc. They have been optically inspected after assembly and subsequently subjected to extreme temperature thermal cycling to assess their reliability for future deep space, long-term, extreme temperature environmental missions. In this study, the employed temperature range (-185°C to +125°C) covers military specifications (-55°C to +100°C), extreme cold Martian (-120°C to +115°C), asteroid Nereus (-180°C to +25°C) and JUNO (-150°C to +120°C) environments. The boards were inspected at room temperature and at various intervals as a function of extreme temperature thermal cycling and bake duration. Electrical resistance measurements made at room temperature are reported and the tests to date have shown some change in resistance as a function of extreme temperature thermal cycling and some showed increase in resistance. However, the change in interconnect resistance becomes more noticeable with increasing number of thermal cycles. Further research work will be carried out to understand the reliability of packages under extreme temperature applications (-185°C to +125°C) via continuously monitoring the daisy chain resistance for BGA, Flat-packs, lead less chip packages, etc. This paper will describe the experimental reliability results of miniaturized passive components (01005, 0201, 0402, 0603, 0805, and 1206) assembled using surface mounting processes with tin-lead and lead-free solder alloys under extreme temperature environments.

Microbial communities of Hyper saline Lake Salda and Acigol, SW Turkey and Their effects on Biogeochemical cycles

NASA Astrophysics Data System (ADS)

Balci, N.; Karaguler, N. G.; Ece, I.; Romanek, C.

2009-12-01

The modern lakes Acigol and Salda, located in the “Lake District” of SW of Turkey, are known for the precipitation of sodium, magnesium, and potassium salts, and Mg-rich carbonate, respectively. As an analogue to extraterrestrial environments, these lakes provide opportunities to study microbe-mineral interactions in extreme environments, and in turn to better understand biogeochemical conditions in such environments. Lake Salda is an evaporatic alkaline lake (pH: 9) that covers an area of about 45 km2 in a partially serpentinized ophiolitic rocks. Water samples collected from the surface contain c. 295 mg/L Mg and c. 190 mg/L Na at a pH of 9.1, while the stream entering the lake (pH range 7-9.5) had values of 55 mg/L and 3 mg/L, respectively, indicating significant Na enrichment relative to Mg in the lake. Microbiological analyses of sediment samples from the stream and the lake indicate a diverse microbial community. Lake Acigol is a perennial lake with a maximum salinity of about 200 g/L and covers an area of 55-60 km2 . Water samples were taken from the lake and ponds around the lake in addition to sediment samples. The water chemistry revealed relatively high Na and SO4 concentrations both in the lake (30 gr/L, 33.36 gr/L), and the ponds (100 mg/L, 123 mg/L). The mineralogical analyses of sediments showed gypsum, halite, carbonate (aragonite, huntite) precipitation in the lake and ponds. The geochemical and microbiological data from both lakes suggest that the metabolic activity of microorganisms (cyanobacteria, sulfate reducing bacteria) significantly affect the surrounding microenvironment, overcoming the common kinetic inhibitors to carbonate mineral precipitation by raising the pH and Mg- and HCO3-ion concentration, and by reducing sulfate ion concentration of the waters. We are currently undertaking laboratory experiments to elucidate biological influences on the precipitation of carbonate minerals under field conditions.

Open-water and under-ice seasonal variations in trace element content and physicochemical associations in fluvial bed sediment.

PubMed

Doig, Lorne E; Carr, Meghan K; Meissner, Anna G N; Jardine, Tim D; Jones, Paul D; Bharadwaj, Lalita; Lindenschmidt, Karl-Erich

2017-11-01

Across the circumpolar world, intensive anthropogenic activities in the southern reaches of many large, northward-flowing rivers can cause sediment contamination in the downstream depositional environment. The influence of ice cover on concentrations of inorganic contaminants in bed sediment (i.e., sediment quality) is unknown in these rivers, where winter is the dominant season. A geomorphic response unit approach was used to select hydraulically diverse sampling sites across a northern test-case system, the Slave River and delta (Northwest Territories, Canada). Surface sediment samples (top 1 cm) were collected from 6 predefined geomorphic response units (12 sites) to assess the relationships between bed sediment physicochemistry (particle size distribution and total organic carbon content) and trace element content (mercury and 18 other trace elements) during open-water conditions. A subset of sites was resampled under-ice to assess the influence of season on these relationships and on total trace element content. Concentrations of the majority of trace elements were strongly correlated with percent fines and proxies for grain size (aluminum and iron), with similar trace element grain size/grain size proxy relationships between seasons. However, finer materials were deposited under ice with associated increases in sediment total organic carbon content and the concentrations of most trace elements investigated. The geomorphic response unit approach was effective at identifying diverse hydrological environments for sampling prior to field operations. Our data demonstrate the need for under-ice sampling to confirm year-round consistency in trace element-geochemical relationships in fluvial systems and to define the upper extremes of these relationships. Whether contaminated or not, under-ice bed sediment can represent a "worst-case" scenario in terms of trace element concentrations and exposure for sediment-associated organisms in northern fluvial systems. Environ Toxicol Chem 2017;36:2916-2924. © 2017 SETAC. © 2017 SETAC.

Astrobiology: Life in Extreme Environments

ERIC Educational Resources Information Center

Kaur, Preeti

2011-01-01

Astrobiology is the study of the origin, evolution and distribution of life in the universe. It seeks to answer two important scientific questions: how did we get here and are we alone in the universe? Scientists begin by studying life on Earth and its limits. The discovery of extremophiles on Earth capable of surviving extremes encourages the…

High-temperature Y267 EPDM elastomer: field and laboratory experiences, August 1981

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hirasuna, A.R.; Friese, G.J.; Stephens, C.A.

1982-03-01

Experiences which indicate the superiority of Y267 EPDM elastomer for high-temperature brines and other environments uses are summarized. Its good processing qualities, extremely good thermochemical stability, extremely good mechanical properties, its low-cost constituents, and its good performance in hydrocarbons are described in some case histories. (MCW)

Reliability of Powertrain Components Exposed to Extreme Tribological Environments

DTIC Science & Technology

2010-08-17

Tribological Environments, Fenske et al. UNCLASSIFIED: Dist A. Approved for public release 2010 NDIA GROUND VEHICLE SYSTEMS ENGINEERING AND...TRIBOLOGICAL ENVIRONMENTS G. R. Fenske , O. O. Ajayi, R. A. Erck, C. Lorenzo-Martin, and Ashley Masoner Energy Systems Division Argonne National...Tribological Environments, Fenske et al. UNCLASSIFIED: Dist A. Approved for public release presented below summarize a series of tests that was performed

Min and Max Exponential Extreme Interval Values and Statistics

ERIC Educational Resources Information Center

Jance, Marsha; Thomopoulos, Nick

2009-01-01

The extreme interval values and statistics (expected value, median, mode, standard deviation, and coefficient of variation) for the smallest (min) and largest (max) values of exponentially distributed variables with parameter ? = 1 are examined for different observation (sample) sizes. An extreme interval value g[subscript a] is defined as a…

Metagenomic Analysis of Hot Springs in Central India Reveals Hydrocarbon Degrading Thermophiles and Pathways Essential for Survival in Extreme Environments.

PubMed

Saxena, Rituja; Dhakan, Darshan B; Mittal, Parul; Waiker, Prashant; Chowdhury, Anirban; Ghatak, Arundhuti; Sharma, Vineet K

2016-01-01

Extreme ecosystems such as hot springs are of great interest as a source of novel extremophilic species, enzymes, metabolic functions for survival and biotechnological products. India harbors hundreds of hot springs, the majority of which are not yet explored and require comprehensive studies to unravel their unknown and untapped phylogenetic and functional diversity. The aim of this study was to perform a large-scale metagenomic analysis of three major hot springs located in central India namely, Badi Anhoni, Chhoti Anhoni, and Tattapani at two geographically distinct regions (Anhoni and Tattapani), to uncover the resident microbial community and their metabolic traits. Samples were collected from seven distinct sites of the three hot spring locations with temperature ranging from 43.5 to 98°C. The 16S rRNA gene amplicon sequencing of V3 hypervariable region and shotgun metagenome sequencing uncovered a unique taxonomic and metabolic diversity of the resident thermophilic microbial community in these hot springs. Genes associated with hydrocarbon degradation pathways, such as benzoate, xylene, toluene, and benzene were observed to be abundant in the Anhoni hot springs (43.5-55°C), dominated by Pseudomonas stutzeri and Acidovorax sp., suggesting the presence of chemoorganotrophic thermophilic community with the ability to utilize complex hydrocarbons as a source of energy. A high abundance of genes belonging to methane metabolism pathway was observed at Chhoti Anhoni hot spring, where methane is reported to constitute >80% of all the emitted gases, which was marked by the high abundance of Methylococcus capsulatus . The Tattapani hot spring, with a high-temperature range (61.5-98°C), displayed a lower microbial diversity and was primarily dominated by a nitrate-reducing archaeal species Pyrobaculum aerophilum . A higher abundance of cell metabolism pathways essential for the microbial survival in extreme conditions was observed at Tattapani. Taken together, the results of this study reveal a novel consortium of microbes, genes, and pathways associated with the hot spring environment.

Computer game-based upper extremity training in the home environment in stroke persons: a single subject design.

PubMed

Slijper, Angelique; Svensson, Karin E; Backlund, Per; Engström, Henrik; Sunnerhagen, Katharina Stibrant

2014-03-13

The objective of the present study was to assess whether computer game-based training in the home setting in the late phase after stroke could improve upper extremity motor function. Twelve subjects with prior stroke were recruited; 11 completed the study. The study had a single subject design; there was a baseline test (A1), a during intervention test (B) once a week, a post-test (A2) measured directly after the treatment phase, plus a follow-up (C) 16-18 weeks after the treatment phase. Information on motor function (Fugl-Meyer), grip force (GrippitR) and arm function in activity (ARAT, ABILHAND) was gathered at A1, A2 and C. During B, only Fugl-Meyer and ARAT were measured. The intervention comprised five weeks of game-based computer training in the home environment. All games were designed to be controlled by either the affected arm alone or by both arms. Conventional formulae were used to calculate the mean, median and standard deviations. Wilcoxon's signed rank test was used for tests of dependent samples. Continuous data were analyzed by methods for repeated measures and ordinal data were analyzed by methods for ordered multinomial data using cumulative logistic models. A p-value of < 0.05 was considered statistically significant. Six females and five males, participated in the study with an average age of 58 years (range 26-66). FMA-UE A-D (motor function), ARAT, the maximal grip force and the mean grip force on the affected side show significant improvements at post-test and follow-up compared to baseline. No significant correlation was found between the amount of game time and changes in the outcomes investigated in this study. The results indicate that computer game-based training could be a promising approach to improve upper extremity function in the late phase after stroke, since in this study, changes were achieved in motor function and activity capacity.

A Systematic Approach for Engagement Analysis Under Multitasking Environments

NASA Technical Reports Server (NTRS)

Zhang, Guangfan; Leddo, John; Xu, Roger; Richey, Carl; Schnell, Tom; McKenzie, Frederick; Li, Jiang

2011-01-01

An overload condition can lead to high stress for an operator and further cause substantial drops in performance. On the other extreme, in automated systems, an operator may become underloaded; in which case, it is difficult for the operator to maintain sustained attention. When an unexpected event occurs, either internal or external to the automated system, a disengaged operation may neglect, misunderstand, or respond slowly/inappropriately to the situation. In this paper, we discuss a systematic approach monitor for extremes of cognitive workload and engagement in multitasking environments. Inferences of cognitive workload ar engagement are based on subjective evaluations, objective performance measures, physiological signals, and task analysis results. The systematic approach developed In this paper aggregates these types of information collected under the multitasking environment and can provide a real-time assessment or engagement.

Vision Aspects of Space Flight

NASA Technical Reports Server (NTRS)

Manuel, Keith; Billica, Roger (Technical Monitor)

2000-01-01

Vision, being one of our most important senses, is critically important in the unique working environment of space flight. Critical evaluation of the astronauts visual system begins with pre-selection examinations resulting in an average of 65% of all medical disqualification's caused by ocular findings. With an average age of 42, approximately 60% of the astronaut corps requires vision correction. Further demands of the unique training and working environment of microgravity, variable lighting from very poor to extreme brightness of sunlight and exposure to extremes of electromagnetic energy results in unique eyewear and contact lens applications. This presentation will describe some of those unique eyewear and contact lens applications used in space flight and training environments. Additionally, ocular findings from 26 shuttle and 5 MIR mission post-flight examinations will be presented.

Bacillus Endospores Isolated from Granite: Close Molecular Relationships to Globally Distributed Bacillus spp. from Endolithic and Extreme Environments

PubMed Central

Fajardo-Cavazos, Patricia; Nicholson, Wayne

2006-01-01

As part of an ongoing effort to catalog spore-forming bacterial populations in environments conducive to interplanetary transfer by natural impacts or by human spaceflight activities, spores of Bacillus spp. were isolated and characterized from the interior of near-subsurface granite rock collected from the Santa Catalina Mountains, AZ. Granite was found to contain ∼500 cultivable Bacillus spores and ∼104 total cultivable bacteria per gram. Many of the Bacillus isolates produced a previously unreported diffusible blue fluorescent compound. Two strains of eight tested exhibited increased spore UV resistance relative to a standard Bacillus subtilis UV biodosimetry strain. Fifty-six isolates were identified by repetitive extragenic palindromic PCR (rep-PCR) and 16S rRNA gene analysis as most closely related to B. megaterium (15 isolates), B. simplex (23 isolates), B. drentensis (6 isolates), B. niacini (7 isolates), and, likely, a new species related to B. barbaricus (5 isolates). Granite isolates were very closely related to a limited number of Bacillus spp. previously found to inhabit (i) globally distributed endolithic sites such as biodeteriorated murals, stone tombs, underground caverns, and rock concretions and (ii) extreme environments such as Antarctic soils, deep sea floor sediments, and spacecraft assembly facilities. Thus, it appears that the occurrence of Bacillus spp. in endolithic or extreme environments is not accidental but that these environments create unique niches excluding most Bacillus spp. but to which a limited number of Bacillus spp. are specifically adapted. PMID:16597992

Design and optimization of a modular setup for measurements of three-dimensional spin polarization with ultrafast pulsed sources.

PubMed

Pincelli, T; Petrov, V N; Brajnik, G; Ciprian, R; Lollobrigida, V; Torelli, P; Krizmancic, D; Salvador, F; De Luisa, A; Sergo, R; Gubertini, A; Cautero, G; Carrato, S; Rossi, G; Panaccione, G

2016-03-01

ULTRASPIN is an apparatus devoted to the measurement of the spin polarization (SP) of electrons ejected from solid surfaces in a UHV environment. It is designed to exploit ultrafast light sources (free electron laser or laser high harmonic generation) and to perform (photo)electron spin analysis by an arrangement of Mott scattering polarimeters that measure the full SP vector. The system consists of two interconnected UHV vessels: one for surface science sample cleaning treatments, e-beam deposition of ultrathin films, and low energy electron diffraction/AES characterization. The sample environment in the polarimeter allows for cryogenic cooling and in-operando application of electric and magnetic fields. The photoelectrons are collected by an electrostatic accelerator and transport lens that form a periaxial beam that is subsequently directed by a Y-shaped electrostatic deflector to either one of the two orthogonal Mott polarimeters. The apparatus has been designed to operate in the extreme conditions of ultraintense single-X-ray pulses as originated by free electron lasers (up to 1 kHz), but it allows also for the single electron counting mode suitable when using statistical sources such as synchrotron radiation, cw-laser, or e-gun beams (up to 150 kcps).

Design and optimization of a modular setup for measurements of three-dimensional spin polarization with ultrafast pulsed sources

NASA Astrophysics Data System (ADS)

Pincelli, T.; Petrov, V. N.; Brajnik, G.; Ciprian, R.; Lollobrigida, V.; Torelli, P.; Krizmancic, D.; Salvador, F.; De Luisa, A.; Sergo, R.; Gubertini, A.; Cautero, G.; Carrato, S.; Rossi, G.; Panaccione, G.

2016-03-01

ULTRASPIN is an apparatus devoted to the measurement of the spin polarization (SP) of electrons ejected from solid surfaces in a UHV environment. It is designed to exploit ultrafast light sources (free electron laser or laser high harmonic generation) and to perform (photo)electron spin analysis by an arrangement of Mott scattering polarimeters that measure the full SP vector. The system consists of two interconnected UHV vessels: one for surface science sample cleaning treatments, e-beam deposition of ultrathin films, and low energy electron diffraction/AES characterization. The sample environment in the polarimeter allows for cryogenic cooling and in-operando application of electric and magnetic fields. The photoelectrons are collected by an electrostatic accelerator and transport lens that form a periaxial beam that is subsequently directed by a Y-shaped electrostatic deflector to either one of the two orthogonal Mott polarimeters. The apparatus has been designed to operate in the extreme conditions of ultraintense single-X-ray pulses as originated by free electron lasers (up to 1 kHz), but it allows also for the single electron counting mode suitable when using statistical sources such as synchrotron radiation, cw-laser, or e-gun beams (up to 150 kcps).

Hand Washing Induces a Clean Slate Effect in Moral Judgments: A Pupillometry and Eye-Tracking Study

PubMed Central

Kaspar, Kai; Krapp, Vanessa; König, Peter

2015-01-01

Physical cleansing is commonly understood to protect us against physical contamination. However, recent studies showed additional effects on moral judgments. Under the heading of the “Macbeth effect” direct links between bodily cleansing and one’s own moral purity have been demonstrated. Here we investigate (1) how moral judgments develop over time and how they are altered by hand washing, (2) whether changes in moral judgments can be explained by altered information sampling from the environment, and (3) whether hand washing affects emotional arousal. Using a pre-post control group design, we found that morality ratings of morally good and bad scenes acquired more extreme values in the control group over time, an effect that was fully counteracted by intermediate hand washing. This result supports the notion of a clean slate effect by hand washing. Thereby, eye-tracking data did not uncover differences in eye movement behavior that may explain differences in moral judgments. Thus, the clean slate effect is not due to altered information sampling from the environment. Finally, compared to the control group, pupil diameter decreased after hand washing, thus demonstrating a direct physiological effect. The results shed light on the physiological mechanisms behind this type of embodiment phenomenon. PMID:25994083

Impact-generated endolithic habitat within crystalline rocks of the Haughton impact structure, Devon Island, Canada.

PubMed

Pontefract, Alexandra; Osinski, Gordon R; Cockell, Charles S; Moore, Casey A; Moores, John E; Southam, Gordon

2014-06-01

The colonization of rocks by endolithic communities is an advantageous trait, especially in environments such as hot or cold deserts, where large temperature ranges, low water availability, and high-intensity ultraviolet radiation pose a significant challenge to survival and growth. On Mars, similar conditions (albeit more extreme) prevail. In these environments, meteorite impact structures could provide refuge for endolithic organisms. Though initially detrimental to biology, an impact event into a rocky body can favorably change the availability and habitability of a substrate for endolithic organisms, which are then able to (re)colonize microfractures and pore spaces created during the impact. Here, we show how shocked gneisses from the Haughton impact structure, Devon Island, Canada, offer significant refuge for endolithic communities. A total of 28 gneiss samples representing a range of shock states were analyzed, collected from in situ, stable field locations. For each sample, the top centimeter of rock was examined with confocal scanning laser microscopy, scanning electron microscopy, and bright-field microscopy to investigate the relationship of biomass with shock level, which was found to correlate generally with increased shock state and particularly with increased porosity. We found that gneisses, which experienced pressures between 35 and 60 GPa, provide the most ideal habitat for endolithic organisms.

Fetal exposures and perinatal influences on the stool microbiota of premature infants.

PubMed

Chernikova, Diana A; Koestler, Devin C; Hoen, Anne Gatewood; Housman, Molly L; Hibberd, Patricia L; Moore, Jason H; Morrison, Hilary G; Sogin, Mitchell L; Zain-Ul-Abideen, Muhammad; Madan, Juliette C

2016-01-01

To test the hypothesis that maternal complications significantly affect gut colonization patterns in very low birth weight infants. Forty-nine serial stool samples were obtained weekly from nine extremely premature infants enrolled in a prospective longitudinal study. Sequencing of the bacterial 16S rRNA gene from stool samples was performed to approximate the intestinal microbiome. Linear mixed effects models were used to evaluate relationships between perinatal complications and intestinal microbiome development. Subjects with prenatal exposure to a non-sterile intrauterine environment, i.e. prolonged preterm premature rupture of membranes (PPPROM) and chorioamnionitis exposure, were found to have a relatively higher abundance of potentially pathogenic bacteria in the stool across all time points compared to subjects without those exposures, irrespective of exposure to postnatal antibiotics. Compared with those delivered by Caesarean section, vaginally delivered subjects were found to have significantly lower diversity of stool microbiota across all time points, with lower abundance of many genera, most in the family Enterobacteriaceae. We identified persistently increased potential pathogen abundance in the developing stool microbiota of subjects exposed to a non-sterile uterine environment. Maternal complications appear to significantly influence the diversity and bacterial composition of the stool microbiota of premature infants, with findings persisting over time.

Design and optimization of a modular setup for measurements of three-dimensional spin polarization with ultrafast pulsed sources

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pincelli, T., E-mail: pincelli@iom.cnr.it; Rossi, G.; Laboratorio TASC, IOM-CNR, S.S. 14 km 163.5, Basovizza, 34149 Trieste

2016-03-15

ULTRASPIN is an apparatus devoted to the measurement of the spin polarization (SP) of electrons ejected from solid surfaces in a UHV environment. It is designed to exploit ultrafast light sources (free electron laser or laser high harmonic generation) and to perform (photo)electron spin analysis by an arrangement of Mott scattering polarimeters that measure the full SP vector. The system consists of two interconnected UHV vessels: one for surface science sample cleaning treatments, e-beam deposition of ultrathin films, and low energy electron diffraction/AES characterization. The sample environment in the polarimeter allows for cryogenic cooling and in-operando application of electric andmore » magnetic fields. The photoelectrons are collected by an electrostatic accelerator and transport lens that form a periaxial beam that is subsequently directed by a Y-shaped electrostatic deflector to either one of the two orthogonal Mott polarimeters. The apparatus has been designed to operate in the extreme conditions of ultraintense single-X-ray pulses as originated by free electron lasers (up to 1 kHz), but it allows also for the single electron counting mode suitable when using statistical sources such as synchrotron radiation, cw-laser, or e-gun beams (up to 150 kcps).« less

Ridge-flank crustal microbiology investigated with long-term borehole observatories

NASA Astrophysics Data System (ADS)

Orcutt, B. N.; Bach, W.; Becker, K.; Edwards, K. J.; Fisher, A. T.; Haddad, A.; Hulme, S.; Teske, A.; Toner, B.; Wheat, C. G.

2011-12-01

The ridge flank environment represents an important habitat for microbial life on Earth, considering its size and chemical disequilibria between circulating fluids and rocks. However, the potential for this habitat to harbor life, and the characteristics that such life might have, are poorly known at present. Furthermore, the interactions of microbial communities across deep sediment-basement interfaces are not well-characterized. Subseafloor borehole observatories provide a novel platform for sampling and monitoring the microbiology of the crustal ridge flank environment. We present current results from a series of subsurface microbial colonization experiments using borehole observatories on the eastern flank of the Juan de Fuca Ridge, as well as analysis of samples collected on a transect away from a seawater-recharging seamount on this ridge flank. These results are compared to the microbiology of observatories installed in the Costa Rica Rift flank with similar fluid composition and temperatures (i.e. anoxic and warm). We will also discuss on-going experiments on the western-flank of the Mid-Atlantic Ridge, where formation fluids in basement are oxic and cool. Results from these experiments represent some of the extremes in crustal fluid conditions, paving the way for additional studies that are needed to address the importance of this biome as a carbon reservoir and a mechanism for crustal alteration.

Mother-infant interactions and regional brain volumes in infancy: an MRI study.

PubMed

Sethna, Vaheshta; Pote, Inês; Wang, Siying; Gudbrandsen, Maria; Blasi, Anna; McCusker, Caroline; Daly, Eileen; Perry, Emily; Adams, Kerrie P H; Kuklisova-Murgasova, Maria; Busuulwa, Paula; Lloyd-Fox, Sarah; Murray, Lynne; Johnson, Mark H; Williams, Steven C R; Murphy, Declan G M; Craig, Michael C; McAlonan, Grainne M

2017-07-01

It is generally agreed that the human brain is responsive to environmental influences, and that the male brain may be particularly sensitive to early adversity. However, this is largely based on retrospective studies of older children and adolescents exposed to extreme environments in childhood. Less is understood about how normative variations in parent-child interactions are associated with the development of the infant brain in typical settings. To address this, we used magnetic resonance imaging to investigate the relationship between observational measures of mother-infant interactions and regional brain volumes in a community sample of 3- to 6-month-old infants (N = 39). In addition, we examined whether this relationship differed in male and female infants. We found that lower maternal sensitivity was correlated with smaller subcortical grey matter volumes in the whole sample, and that this was similar in both sexes. However, male infants who showed greater levels of positive communication and engagement during early interactions had smaller cerebellar volumes. These preliminary findings suggest that variations in mother-infant interaction dimensions are associated with differences in infant brain development. Although the study is cross-sectional and causation cannot be inferred, the findings reveal a dynamic interaction between brain and environment that may be important when considering interventions to optimize infant outcomes.

Ratiometric Phosphorescent Probe for Thallium in Serum, Water, and Soil Samples Based on Long-Lived, Spectrally Resolved, Mn-Doped ZnSe Quantum Dots and Carbon Dots.

PubMed

Lu, Xiaomei; Zhang, Jinyi; Xie, Ya-Ni; Zhang, Xinfeng; Jiang, Xiaoming; Hou, Xiandeng; Wu, Peng

2018-02-20

Thallium (Tl) is an extremely toxic heavy metal and exists in very low concentrations in the environment, but its sensing is largely underexplored as compared to its neighboring elements in the periodic table (especially mercury and lead). In this work, we developed a ratiometric phosphorescent nanoprobe for thallium detection based on Mn-doped ZnSe quantum dots (QDs) and water-soluble carbon dots (C-dots). Upon excitation with 360 nm, Mn-doped ZnSe QDs and C-dots can emit long-lived and spectrally resolved phosphorescence at 580 and 440 nm, respectively. In the presence of thallium, the phosphorescence emission from Mn-doped ZnSe QDs could be selectively quenched, while that from C-dots retained unchanged. Therefore, a ratiometric phosphorescent probe was thus developed, which can eliminate the potential influence from both background fluorescence and other analyte-independent external environment factors. Several other heavy metal ions caused interferences to thallium detection but could be efficiently masked with EDTA. The proposed method offered a detection limit of 1 μg/L, which is among the most sensitive probes ever reported. Successful application of this method for thallium detection in biological serum as well as in environmental water and soil samples was demonstrated.

Potential Physiologies of Deep Branches on the Tree of Life with Deep Subsurface Samples from IODP Leg 347: Baltic Sea Paleoenvironment

NASA Astrophysics Data System (ADS)

Lloyd, K. G.; Bird, J. T.; Shumaker, A.

2014-12-01

Very little is known about how evolutionary branches that are distantly related to cultured microorganisms make a living in the deep subsurface marine environment. Here, sediments are cut-off from surface inputs of organic substrates for tens of thousands of years; yet somehow support a diverse population of microorganisms. We examined the potential metabolic and ecological roles of uncultured archaea and bacteria in IODP Leg 347: Baltic Sea Paleoenvironment samples, using quantitative PCR holes 60B, 63E, 65C, and 59C and single cell genomic analysis for hole 60B. We quantified changes in total archaea and bacteria, as well as deeply-branching archaeal taxa with depth. These sediment cores alternate between high and low salinities, following a glacial cycle. This allows changes in the quantities of these groups to be placed in the context of potentially vastly different organic matter sources. In addition, single cells were isolated, and their genomes were amplified and sequenced to allow a deeper look into potential physiologies of uncultured deeply-branching organisms found up to 86 meters deep in marine sediments. Together, these data provide deeper insight into the relationship between microorganisms and their organic matter substrates in this extreme environments.

Radiation attenuation by single-crystal diamond windows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guthrie, M.; Pruteanu, C. G.; Donnelly, M. -E.

As artificial diamond becomes more cost effective it is likely to see increasing use as a window for sample environment equipment used in diffraction experiments. Such windows are particularly useful as they exhibit exceptional mechanical properties in addition to being highly transparent to both X-ray and neutron radiation. A key application is in high-pressure studies, where diamond anvil cells (DACs) are used to access extreme sample conditions. However, despite their utility, an important consideration when using single-crystal diamond windows is their interaction with the incident beam. In particular, the Bragg condition will be satisfied for specific angles and wavelengths, leadingmore » to the appearance of diamond Bragg spots on the diffraction detectors but also, unavoidably, to loss of transmitted intensity of the beam that interacts with the sample. This effect can be particularly significant for energy-dispersive measurements, for example, in time-of-flight neutron diffraction work using DACs. This article presents a semi-empirical approach that can be used to correct for this effect, which is a prerequisite for the accurate determination of diffraction intensities.« less

Radiation attenuation by single-crystal diamond windows

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guthrie, Malcolm; Pruteanu, Ciprian G.; Donnelly, Mary -Ellen

As artificial diamond becomes more cost effective it is likely to see increasing use as a window for sample environment equipment used in diffraction experiments. Such windows are particularly useful as they exhibit exceptional mechanical properties in addition to being highly transparent to both X-ray and neutron radiation. A key application is in high-pressure studies, where diamond anvil cells (DACs) are used to access extreme sample conditions. However, despite their utility, an important consideration when using single-crystal diamond windows is their interaction with the incident beam. In particular, the Bragg condition will be satisfied for specific angles and wavelengths, leadingmore » to the appearance of diamond Bragg spots on the diffraction detectors but also, unavoidably, to loss of transmitted intensity of the beam that interacts with the sample. This effect can be particularly significant for energy-dispersive measurements, for example, in time-of-flight neutron diffraction work using DACs. Furthermore, this article presents a semi-empirical approach that can be used to correct for this effect, which is a prerequisite for the accurate determination of diffraction intensities.« less

Radiation attenuation by single-crystal diamond windows

DOE PAGES

Guthrie, Malcolm; Pruteanu, Ciprian G.; Donnelly, Mary -Ellen; ...

2017-02-01

As artificial diamond becomes more cost effective it is likely to see increasing use as a window for sample environment equipment used in diffraction experiments. Such windows are particularly useful as they exhibit exceptional mechanical properties in addition to being highly transparent to both X-ray and neutron radiation. A key application is in high-pressure studies, where diamond anvil cells (DACs) are used to access extreme sample conditions. However, despite their utility, an important consideration when using single-crystal diamond windows is their interaction with the incident beam. In particular, the Bragg condition will be satisfied for specific angles and wavelengths, leadingmore » to the appearance of diamond Bragg spots on the diffraction detectors but also, unavoidably, to loss of transmitted intensity of the beam that interacts with the sample. This effect can be particularly significant for energy-dispersive measurements, for example, in time-of-flight neutron diffraction work using DACs. Furthermore, this article presents a semi-empirical approach that can be used to correct for this effect, which is a prerequisite for the accurate determination of diffraction intensities.« less

Melting dynamics of ice in the mesoscopic regime

PubMed Central

Citroni, Margherita; Fanetti, Samuele; Falsini, Naomi; Foggi, Paolo; Bini, Roberto

2017-01-01

How does a crystal melt? How long does it take for melt nuclei to grow? The melting mechanisms have been addressed by several theoretical and experimental works, covering a subnanosecond time window with sample sizes of tens of nanometers and thus suitable to determine the onset of the process but unable to unveil the following dynamics. On the other hand, macroscopic observations of phase transitions, with millisecond or longer time resolution, account for processes occurring at surfaces and time limited by thermal contact with the environment. Here, we fill the gap between these two extremes, investigating the melting of ice in the entire mesoscopic regime. A bulk ice Ih or ice VI sample is homogeneously heated by a picosecond infrared pulse, which delivers all of the energy necessary for complete melting. The evolution of melt/ice interfaces thereafter is monitored by Mie scattering with nanosecond resolution, for all of the time needed for the sample to reequilibrate. The growth of the liquid domains, over distances of micrometers, takes hundreds of nanoseconds, a time orders of magnitude larger than expected from simple H-bond dynamics. PMID:28536197

Communities that thrive in extreme conditions captured from a freshwater lake.

PubMed

Low-Décarie, Etienne; Fussmann, Gregor F; Dumbrell, Alex J; Bell, Graham

2016-09-01

Organisms that can grow in extreme conditions would be expected to be confined to extreme environments. However, we were able to capture highly productive communities of algae and bacteria capable of growing in acidic (pH 2), basic (pH 12) and saline (40 ppt) conditions from an ordinary freshwater lake. Microbial communities may thus include taxa that are highly productive in conditions that are far outside the range of conditions experienced in their host ecosystem. The organisms we captured were not obligate extremophiles, but were capable of growing in both extreme and benign conditions. The ability to grow in extreme conditions may thus be a common functional attribute in microbial communities. © 2016 The Author(s).

Behavior Prediction Tools Strengthen Nanoelectronics

NASA Technical Reports Server (NTRS)

2013-01-01

Several years ago, NASA started making plans to send robots to explore the deep, dark craters on the Moon. As part of these plans, NASA needed modeling tools to help engineer unique electronics to withstand extremely cold temperatures. According to Jonathan Pellish, a flight systems test engineer at Goddard Space Flight Center, "An instrument sitting in a shadowed crater on one of the Moon s poles would hover around 43 K", that is, 43 kelvin, equivalent to -382 F. Such frigid temperatures are one of the main factors that make the extreme space environments encountered on the Moon and elsewhere so extreme. Radiation is another main concern. "Radiation is always present in the space environment," says Pellish. "Small to moderate solar energetic particle events happen regularly and extreme events happen less than a handful of times throughout the 7 active years of the 11-year solar cycle." Radiation can corrupt data, propagate to other systems, require component power cycling, and cause a host of other harmful effects. In order to explore places like the Moon, Jupiter, Saturn, Venus, and Mars, NASA must use electronic communication devices like transmitters and receivers and data collection devices like infrared cameras that can resist the effects of extreme temperature and radiation; otherwise, the electronics would not be reliable for the duration of the mission.

An adaptation to life in acid through a novel mevalonate pathway

DOE PAGES

Vinokur, Jeffrey M.; Cummins, Matthew C.; Korman, Tyler P.; ...

2016-12-22

Here, extreme acidophiles are capable of growth at pH values near zero. Sustaining life in acidic environments requires extensive adaptations of membranes, proton pumps, and DNA repair mechanisms. Here we describe an adaptation of a core biochemical pathway, the mevalonate pathway, in extreme acidophiles. Two previously known mevalonate pathways involve ATP dependent decarboxylation of either mevalonate 5-phosphate or mevalonate 5-pyrophosphate, in which a single enzyme carries out two essential steps: (1) phosphorylation of the mevalonate moiety at the 3-OH position and (2) subsequent decarboxylation. We now demonstrate that in extreme acidophiles, decarboxylation is carried out by two separate steps: previouslymore » identified enzymes generate mevalonate 3,5-bisphosphate and a new decarboxylase we describe here, mevalonate 3,5-bisphosphate decarboxylase, produces isopentenyl phosphate. Why use two enzymes in acidophiles when one enzyme provides both functionalities in all other organisms examined to date? We find that at low pH, the dual function enzyme, mevalonate 5-phosphate decarboxylase is unable to carry out the first phosphorylation step, yet retains its ability to perform decarboxylation. We therefore propose that extreme acidophiles had to replace the dual-purpose enzyme with two specialized enzymes to efficiently produce isoprenoids in extremely acidic environments.« less

An adaptation to life in acid through a novel mevalonate pathway

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vinokur, Jeffrey M.; Cummins, Matthew C.; Korman, Tyler P.

Here, extreme acidophiles are capable of growth at pH values near zero. Sustaining life in acidic environments requires extensive adaptations of membranes, proton pumps, and DNA repair mechanisms. Here we describe an adaptation of a core biochemical pathway, the mevalonate pathway, in extreme acidophiles. Two previously known mevalonate pathways involve ATP dependent decarboxylation of either mevalonate 5-phosphate or mevalonate 5-pyrophosphate, in which a single enzyme carries out two essential steps: (1) phosphorylation of the mevalonate moiety at the 3-OH position and (2) subsequent decarboxylation. We now demonstrate that in extreme acidophiles, decarboxylation is carried out by two separate steps: previouslymore » identified enzymes generate mevalonate 3,5-bisphosphate and a new decarboxylase we describe here, mevalonate 3,5-bisphosphate decarboxylase, produces isopentenyl phosphate. Why use two enzymes in acidophiles when one enzyme provides both functionalities in all other organisms examined to date? We find that at low pH, the dual function enzyme, mevalonate 5-phosphate decarboxylase is unable to carry out the first phosphorylation step, yet retains its ability to perform decarboxylation. We therefore propose that extreme acidophiles had to replace the dual-purpose enzyme with two specialized enzymes to efficiently produce isoprenoids in extremely acidic environments.« less

Magnetic flux tailoring through Lenz lenses for ultrasmall samples: A new pathway to high-pressure nuclear magnetic resonance

PubMed Central

Meier, Thomas; Wang, Nan; Mager, Dario; Korvink, Jan G.; Petitgirard, Sylvain; Dubrovinsky, Leonid

2017-01-01

A new pathway to nuclear magnetic resonance (NMR) spectroscopy for picoliter-sized samples (including those kept in harsh and extreme environments, particularly in diamond anvil cells) is introduced, using inductively coupled broadband passive electromagnetic lenses, to locally amplify the magnetic field at the isolated sample, leading to an increase in sensitivity. The lenses are adopted for the geometrical restrictions imposed by a toroidal diamond indenter cell and yield signal-to-noise ratios at pressures as high as 72 GPa at initial sample volumes of only 230 pl. The corresponding levels of detection are found to be up to four orders of magnitude lower compared to formerly used solenoidal microcoils. Two-dimensional nutation experiments on long-chained alkanes, CnH2n+2 (n = 16 to 24), as well as homonuclear correlation spectroscopy on thymine, C5H6N2O2, were used to demonstrate the feasibility of this approach for higher-dimensional NMR experiments, with a spectral resolution of at least 2 parts per million. This approach opens up the field of ultrahigh-pressure sciences to one of the most versatile spectroscopic methods available in a pressure range unprecedented up to now. PMID:29230436

Magnetic flux tailoring through Lenz lenses for ultrasmall samples: A new pathway to high-pressure nuclear magnetic resonance.

PubMed

Meier, Thomas; Wang, Nan; Mager, Dario; Korvink, Jan G; Petitgirard, Sylvain; Dubrovinsky, Leonid

2017-12-01

A new pathway to nuclear magnetic resonance (NMR) spectroscopy for picoliter-sized samples (including those kept in harsh and extreme environments, particularly in diamond anvil cells) is introduced, using inductively coupled broadband passive electromagnetic lenses, to locally amplify the magnetic field at the isolated sample, leading to an increase in sensitivity. The lenses are adopted for the geometrical restrictions imposed by a toroidal diamond indenter cell and yield signal-to-noise ratios at pressures as high as 72 GPa at initial sample volumes of only 230 pl. The corresponding levels of detection are found to be up to four orders of magnitude lower compared to formerly used solenoidal microcoils. Two-dimensional nutation experiments on long-chained alkanes, C n H 2 n +2 ( n = 16 to 24), as well as homonuclear correlation spectroscopy on thymine, C 5 H 6 N 2 O 2 , were used to demonstrate the feasibility of this approach for higher-dimensional NMR experiments, with a spectral resolution of at least 2 parts per million. This approach opens up the field of ultrahigh-pressure sciences to one of the most versatile spectroscopic methods available in a pressure range unprecedented up to now.

Changes in climate extremes and their impacts on the natural physical environment: An overview of the IPCC SREX report

NASA Astrophysics Data System (ADS)

Seneviratne, S. I.; Nicholls, N.; Easterling, D.; Goodess, C. M.; Kanae, S.; Kossin, J.; Luo, Y.; Marengo, J.; McInnes, K.; Rahimi, M.; Reichstein, M.; Sorteberg, A.; Vera, C.; Zhang, X.

2012-04-01

In April 2009, the Intergovernmental Panel on Climate Change (IPCC) decided to prepare a new special report with involvement of the UN International Strategy for Disaster Reduction (ISDR) on the topic "Managing the Risks of Extreme Events and Disasters to Advance Climate Change Adaptation" (SREX, http://ipcc-wg2.gov/SREX/). This special report reviews the scientific literature on past and projected changes in weather and climate extremes, and the relevance of such changes to disaster risk reduction and climate change adaptation. The SREX Summary for Policymakers was approved at an IPCC Plenary session on November 14-18, 2011, and the full report is planned for release in February 2012. This presentation will provide an overview on the structure and contents of the SREX, focusing on Chapter 3: "Changes in climate extremes and their impacts on the natural physical environment" [1]. It will in particular present the main findings of the chapter, including differences between the SREX's conclusions and those of the IPCC Fourth Assessment of 2007, and the implications of this new assessment for disaster risk reduction. Finally, aspects relevant to impacts on the biogeochemical cycles will also be addressed. [1] Seneviratne, S.I., N. Nicholls, D. Easterling, C.M. Goodess, S. Kanae, J. Kossin, Y. Luo, J. Marengo, K. McInnes, M. Rahimi, M. Reichstein, A. Sorteberg, C. Vera, and X. Zhang, 2012: Changes in climate extremes and their impacts on the natural physical environment. In: Intergovernmental Panel on Climate Change Special Report on Managing the Risks of Extreme Events and Disasters to Advance Climate Change Adaptation [Field, C. B., Barros, V., Stocker, T.F., Qin, D., Dokken, D., Ebi, K.L., Mastrandrea, M. D., Mach, K. J., Plattner, G.-K., Allen, S. K., Tignor, M. and P. M. Midgley (eds.)]. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA

A new InterRidge Working Group : Biogeochemical Interactions at Deep-sea Vents

NASA Astrophysics Data System (ADS)

Le Bris, N.; Boetius, A.; Tivey, M. K.; Luther, G. W.; German, C. R.; Wenzhoefer, F.; Charlou, J.; Seyfried, W. E.; Fortin, D.; Ferris, G.; Takai, K.; Baross, J. A.

2004-12-01

A new Working Group on `Biogeochemical Interactions at deep-sea vents' has been created at the initiative of the InterRidge programme. This interdisciplinary group comprises experts in chemistry, geochemistry, biogeochemistry, and microbial ecology addressing questions of biogeochemical interactions in different MOR and BAB environments. The past decade has raised major issues concerning the interactions between biotic and abiotic compartments of deep-sea hydrothermal environments and the role they play in the microbial turnover of C, S, N, Fe, fluxes from the geosphere to hydrosphere, the formation of biominerals, the functioning of vent ecosystems and life in extreme environments, the deep-biosphere, and the origin of life. Recent multidisciplinary studies have provided some new insights to these issues. Results of some of these studies will be presented here. They point out the variability and complexity of geobiological systems at vents in space and time and highlight the need for interactions across the fields of chemistry, geochemistry, biogeochemistry, and microbial ecology of hydrothermal environments. Limitation for advances in these fields include the availability of seafloor observation/experimentation time, and of underwater instrumentation allowing quantitative, in situ measurements of chemical and biological fluxes, as well as physical and chemical sensing and sampling along small scale gradients and repeated observation of study sites. The aim of this new Working Group is to strengthen the scientific exchange among chemists, geochemists, biogeochemists and microbial ecologists to favor collaboration in field studies including intercomparison of methods and planning of integrated experiments. The Biogeochemical Interactions working group will also foster development of underwater instrumentation for in situ biogeochemical measurements and microscale sampling, and promote exchange and collaboration with students and scientists of neighboring disciplines, particularly with vent biologists, ecologists and geologists .

MID-INFRARED EVIDENCE FOR ACCELERATED EVOLUTION IN COMPACT GROUP GALAXIES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walker, Lisa May; Johnson, Kelsey E.; Gallagher, Sarah C.

2010-11-15

Compact galaxy groups are at the extremes of the group environment, with high number densities and low velocity dispersions that likely affect member galaxy evolution. To explore the impact of this environment in detail, we examine the distribution in the mid-infrared (MIR) 3.6-8.0 {mu}m color space of 42 galaxies from 12 Hickson compact groups (HCGs) in comparison with several control samples, including the LVL+SINGS galaxies, interacting galaxies, and galaxies from the Coma Cluster. We find that the HCG galaxies are strongly bimodal, with statistically significant evidence for a gap in their distribution. In contrast, none of the other samples showmore » such a marked gap, and only galaxies in the Coma infall region have a distribution that is statistically consistent with the HCGs in this parameter space. To further investigate the cause of the HCG gap, we compare the galaxy morphologies of the HCG and LVL+SINGS galaxies, and also probe the specific star formation rate (SSFR) of the HCG galaxies. While galaxy morphology in HCG galaxies is strongly linked to position with MIR color space, the more fundamental property appears to be the SSFR, or star formation rate normalized by stellar mass. We conclude that the unusual MIR color distribution of HCG galaxies is a direct product of their environment, which is most similar to that of the Coma infall region. In both cases, galaxy densities are high, but gas has not been fully processed or stripped. We speculate that the compact group environment fosters accelerated evolution of galaxies from star-forming and neutral gas-rich to quiescent and neutral gas-poor, leaving few members in the MIR gap at any time.« less

Compact genome of the Antarctic midge is likely an adaptation to an extreme environment.

PubMed

Kelley, Joanna L; Peyton, Justin T; Fiston-Lavier, Anna-Sophie; Teets, Nicholas M; Yee, Muh-Ching; Johnston, J Spencer; Bustamante, Carlos D; Lee, Richard E; Denlinger, David L

2014-08-12

The midge, Belgica antarctica, is the only insect endemic to Antarctica, and thus it offers a powerful model for probing responses to extreme temperatures, freeze tolerance, dehydration, osmotic stress, ultraviolet radiation and other forms of environmental stress. Here we present the first genome assembly of an extremophile, the first dipteran in the family Chironomidae, and the first Antarctic eukaryote to be sequenced. At 99 megabases, B. antarctica has the smallest insect genome sequenced thus far. Although it has a similar number of genes as other Diptera, the midge genome has very low repeat density and a reduction in intron length. Environmental extremes appear to constrain genome architecture, not gene content. The few transposable elements present are mainly ancient, inactive retroelements. An abundance of genes associated with development, regulation of metabolism and responses to external stimuli may reflect adaptations for surviving in this harsh environment.

Compact genome of the Antarctic midge is likely an adaptation to an extreme environment

PubMed Central

Kelley, Joanna L.; Peyton, Justin T.; Fiston-Lavier, Anna-Sophie; Teets, Nicholas M.; Yee, Muh-Ching; Johnston, J. Spencer; Bustamante, Carlos D.; Lee, Richard E.; Denlinger, David L.

2014-01-01

The midge, Belgica antarctica, is the only insect endemic to Antarctica, and thus it offers a powerful model for probing responses to extreme temperatures, freeze tolerance, dehydration, osmotic stress, ultraviolet radiation and other forms of environmental stress. Here we present the first genome assembly of an extremophile, the first dipteran in the family Chironomidae, and the first Antarctic eukaryote to be sequenced. At 99 megabases, B. antarctica has the smallest insect genome sequenced thus far. Although it has a similar number of genes as other Diptera, the midge genome has very low repeat density and a reduction in intron length. Environmental extremes appear to constrain genome architecture, not gene content. The few transposable elements present are mainly ancient, inactive retroelements. An abundance of genes associated with development, regulation of metabolism and responses to external stimuli may reflect adaptations for surviving in this harsh environment. PMID:25118180

Forest-fire model as a supercritical dynamic model in financial systems

NASA Astrophysics Data System (ADS)

Lee, Deokjae; Kim, Jae-Young; Lee, Jeho; Kahng, B.

2015-02-01

Recently large-scale cascading failures in complex systems have garnered substantial attention. Such extreme events have been treated as an integral part of self-organized criticality (SOC). Recent empirical work has suggested that some extreme events systematically deviate from the SOC paradigm, requiring a different theoretical framework. We shed additional theoretical light on this possibility by studying financial crisis. We build our model of financial crisis on the well-known forest fire model in scale-free networks. Our analysis shows a nontrivial scaling feature indicating supercritical behavior, which is independent of system size. Extreme events in the supercritical state result from bursting of a fat bubble, seeds of which are sown by a protracted period of a benign financial environment with few shocks. Our findings suggest that policymakers can control the magnitude of financial meltdowns by keeping the economy operating within reasonable duration of a benign environment.

Atomistic material behavior at extreme pressures

DOE PAGES

Beland, Laurent K.; Osetskiy, Yury N.; Stoller, Roger E.

2016-08-05

Computer simulations are routinely performed to model the response of materials to extreme environments, such as neutron (or ion) irradiation. The latter involves high-energy collisions from which a recoiling atom creates a so-called atomic displacement cascade. These cascades involve coordinated motion of atoms in the form of supersonic shockwaves. These shockwaves are characterized by local atomic pressures >15 GPa and interatomic distances <2 Å. Similar pressures and interatomic distances are observed in other extreme environment, including short-pulse laser ablation, high-impact ballistic collisions and diamond anvil cells. Displacement cascade simulations using four different force fields, with initial kinetic energies ranging frommore » 1 to 40 keV, show that there is a direct relationship between these high-pressure states and stable defect production. An important shortcoming in the modeling of interatomic interactions at these short distances, which in turn determines final defect production, is brought to light.« less

An Overview of 2014 SBIR Phase I and Phase II Materials Structures for Extreme Environments

NASA Technical Reports Server (NTRS)

Nguyen, Hung D.; Steele, Gynelle C.; Morris, Jessica R.

2015-01-01

NASA's Small Business Innovation Research (SBIR) program focuses on technological innovation by investing in development of innovative concepts and technologies to help NASA mission directorates address critical research needs for Agency programs. This report highlights nine of the innovative SBIR 2014 Phase I and Phase II projects that emphasize one of NASA Glenn Research Center's six core competencies-Materials and Structures for Extreme Environments. The technologies cover a wide spectrum of applications such as high temperature environmental barrier coating systems, deployable space structures, solid oxide fuel cells, and self-lubricating hard coatings for extreme temperatures. Each featured technology describes an innovation, technical objective, and highlights NASA commercial and industrial applications. This report provides an opportunity for NASA engineers, researchers, and program managers to learn how NASA SBIR technologies could help their programs and projects, and lead to collaborations and partnerships between the small SBIR companies and NASA that would benefit both.

FastID: Extremely Fast Forensic DNA Comparisons

DTIC Science & Technology

2017-05-19

FastID: Extremely Fast Forensic DNA Comparisons Darrell O. Ricke, PhD Bioengineering Systems & Technologies Massachusetts Institute of...Technology Lincoln Laboratory Lexington, MA USA Darrell.Ricke@ll.mit.edu Abstract—Rapid analysis of DNA forensic samples can have a critical impact on...time sensitive investigations. Analysis of forensic DNA samples by massively parallel sequencing is creating the next gold standard for DNA

Reliability of High I/O High Density CCGA Interconnect Electronic Packages under Extreme Thermal Environment

NASA Technical Reports Server (NTRS)

Ramesham, Rajeshuni

2012-01-01

This paper provides the experimental test results of advanced CCGA packages tested in extreme temperature thermal environments. Standard optical inspection and x-ray non-destructive inspection tools were used to assess the reliability of high density CCGA packages for deep space extreme temperature missions. Ceramic column grid array (CCGA) packages have been increasing in use based on their advantages such as high interconnect density, very good thermal and electrical performances, compatibility with standard surface-mount packaging assembly processes, and so on. CCGA packages are used in space applications such as in logic and microprocessor functions, telecommunications, payload electronics, and flight avionics. As these packages tend to have less solder joint strain relief than leaded packages or more strain relief over lead-less chip carrier packages, the reliability of CCGA packages is very important for short-term and long-term deep space missions. We have employed high density CCGA 1152 and 1272 daisy chained electronic packages in this preliminary reliability study. Each package is divided into several daisy-chained sections. The physical dimensions of CCGA1152 package is 35 mm x 35 mm with a 34 x 34 array of columns with a 1 mm pitch. The dimension of the CCGA1272 package is 37.5 mm x 37.5 mm with a 36 x 36 array with a 1 mm pitch. The columns are made up of 80% Pb/20%Sn material. CCGA interconnect electronic package printed wiring polyimide boards have been assembled and inspected using non-destructive x-ray imaging techniques. The assembled CCGA boards were subjected to extreme temperature thermal atmospheric cycling to assess their reliability for future deep space missions. The resistance of daisy-chained interconnect sections were monitored continuously during thermal cycling. This paper provides the experimental test results of advanced CCGA packages tested in extreme temperature thermal environments. Standard optical inspection and x-ray non-destructive inspection tools were used to assess the reliability of high density CCGA packages for deep space extreme temperature missions. Keywords: Extreme temperatures, High density CCGA qualification, CCGA reliability, solder joint failures, optical inspection, and x-ray inspection.

Investigating the Effects of Simulated Space conditions on Novel Extremely Halophilic Archaea: Halovarius Luteus gen. nov., sp. nov.

NASA Astrophysics Data System (ADS)

Feshangsaz, Niloofar; Van Loon, ing.. Jack J. W. A.; Nazmi, Kamran; Semsarha, Farid

2016-07-01

Studying halophiles from different environments of Earth provide new insights into our search for life in the universe. Haloarchaea show some unique characteristics and physiological adaptations like acidic proteins against harsh environments such as natural brine with salt concentration approaching saturation (5 M) and regions with low active water. These properties make haloarchaea interesting candidate for astrobiological studies. Halovarius luteus gen. nov., sp. nov. a novel extremely halophilic archaeon from Urmia salt lake, in Iran has been chosen to explore its resistance against a series of extreme conditions. The aim of this study is to assess the resistance of strain DA50T under the effects of simulated space conditions like simulated microgravity, hypergravity, and desiccation. In this paper we will discuss the results of these studies where we specifically focus on changes in carotenoid pigments production and whole cell proteome. This is the first report of very novel Iranian archaea in response to extreme space conditions. The pigments were extracted by acetone and methanol. Pigments were analyzed by scanning the absorbance spectrum in the UV-VIS spectrophotometer. And they were separated by TLC. Whole protein from cell lysate supernatant was extracted after lysis with Bacterial Protein Extraction Reagent and fractionated by RP-HPLC using C18 column. Proteome analyzed by electrophoresis (SDS-PAGE), and MALDI-TOF. Carotenoid pigments are formed under different extreme conditions such as dry environment and gravitational changes. Also the protein composition exhibits alterations after exposure to the same conditions. Our conclusion is that pigments and proteins formation depend on the growth circumstances. Halophiles use this as an adaptation to survive under different environmental conditions.

Microbial ecology of extreme environments: Antarctic yeasts and growth in substrate-limited habitats

NASA Technical Reports Server (NTRS)

Vishniac, H. S.

1985-01-01

The high, dry valleys of the Ross Desert of Antarctic, characterized by extremely low temperatures, aridity and a depauperate biota, are used as an analog of the postulated extreme climates of other planetary bodies of the Solar System to test the hypothesis that if life could be supported by Ross, it might be possible where similar conditions prevail. The previously considered sterility of the Ross Desert soil ecosystem has yielded up an indigenous yeast, Cryptoccus vishniacci, which is able to resist the extremes of cold, wet and dry freezing, and long arid periods, while making minimal nutritional demands on the soil.

Observing Human-induced Linkages between Urbanization and Earth's Climate System

NASA Technical Reports Server (NTRS)

Shepherd, J. Marshall; Jin, Menglin

2004-01-01

Urbanization is one of the extreme cases of land use change. Most of world s population has moved to urban areas. Although currently only 1.2% of the land is considered urban, the spatial coverage and density of cities are expected to rapidly increase in the near future. It is estimated that by the year 2025, 60% of the world s population will live in cities. Human activity in urban environments also alters atmospheric composition; impacts components of the water cycle; and modifies the carbon cycle and ecosystems. However, our understanding of urbanization on the total Earth-climate system is incomplete. Better understanding of how the Earth s atmosphere-ocean-land-biosphere components interact as a coupled system and the influence of the urban environment on this climate system is critical. The goal of the 2003 AGU Union session Human-induced climate variations on urban areas: From observations to modeling was to bring together scientists from interdisciplinary backgrounds to discuss the data, scientific approaches and recent results on observing and modeling components of the urban environment with the intent of sampling our current stand and discussing future direction on this topic. Herein, a summary and discussion of the observations component of the session are presented.

Effect of microgravity on stress ethylene and carbon dioxide production in sweet clover (Melilotus alba L.)

NASA Technical Reports Server (NTRS)

Gallegos, Gregory L.; Odom, William R.; Guikema, James A.

1995-01-01

The study of higher plant growth and development in the microgravity (micro-g) environment continues to be a challenge. This is in part a result of the available flight qualified hardware with restrictive closed gas environments. This point is underscored by considering that gas exchange of seedlings grown in microgravity may be further limited owing to a thicker layer of water wicked onto the roots and to the absence of convective mixing. We hypothesized that seedlings grown under such conditions will experience greater hypoxia in microgravity than at Earth gravity, and thus produce greater stress ethylene. We compared flight and ground samples of sweet clover seedlings grown in the Fluid Processing Apparatus (FPA) during STS-57 and found them to contain extremely high levels of carbon dioxide (CO2) and stress ethylene. There were time dependent increases for both gases, and seedling growth was greatly inhibited. We repeated these experiments aboard STS-60 using modified chambers which increased, by fifty fold, the air available to the developing seedlings. Sweet clover seed germination and subsequent seedling growth to eight days within the FPA modified with a gas permeable membrane is not compromised by the microgravity environment.

Applying Incremental Sampling Methodology to Soils Containing Heterogeneously Distributed Metallic Residues to Improve Risk Analysis.

PubMed

Clausen, J L; Georgian, T; Gardner, K H; Douglas, T A

2018-01-01

This study compares conventional grab sampling to incremental sampling methodology (ISM) to characterize metal contamination at a military small-arms-range. Grab sample results had large variances, positively skewed non-normal distributions, extreme outliers, and poor agreement between duplicate samples even when samples were co-located within tens of centimeters of each other. The extreme outliers strongly influenced the grab sample means for the primary contaminants lead (Pb) and antinomy (Sb). In contrast, median and mean metal concentrations were similar for the ISM samples. ISM significantly reduced measurement uncertainty of estimates of the mean, increasing data quality (e.g., for environmental risk assessments) with fewer samples (e.g., decreasing total project costs). Based on Monte Carlo resampling simulations, grab sampling resulted in highly variable means and upper confidence limits of the mean relative to ISM.

A Novel Adaptation Mechanism Underpinning Algal Colonization of a Nuclear Fuel Storage Pond.

PubMed

MeGraw, Victoria E; Brown, Ashley R; Boothman, Christopher; Goodacre, Royston; Morris, Katherine; Sigee, David; Anderson, Lizzie; Lloyd, Jonathan R

2018-06-26

Geochemical analyses alongside molecular techniques were used to characterize the microbial ecology and biogeochemistry of an outdoor spent nuclear fuel storage pond at Sellafield, United Kingdom, that is susceptible to seasonal algal blooms that cause plant downtime. 18S rRNA gene profiling of the filtered biomass samples showed the increasing dominance of a species closely related to the alga Haematococcus pluvialis , alongside 16S rRNA genes affiliated with a diversity of freshwater bacteria, including Proteobacteria and Cyanobacteria High retention of 137 Cs and 90 Sr on pond water filters coincided with high levels of microbial biomass in the pond, suggesting that microbial colonization may have an important control on radionuclide fate in the pond. To interpret the unexpected dominance of Haematococcus species during bloom events in this extreme environment, the physiological response of H. pluvialis to environmentally relevant ionizing radiation doses was assessed. Irradiated laboratory cultures produced significant quantities of the antioxidant astaxanthin, consistent with pigmentation observed in pond samples. Fourier transform infrared (FT-IR) spectroscopy suggested that radiation did not have a widespread impact on the metabolic fingerprint of H. pluvialis in laboratory experiments, despite the 80-Gy dose. This study suggests that the production of astaxanthin-rich encysted cells may be related to the preservation of the Haematococcus phenotype, potentially allowing it to survive oxidative stress arising from radiation doses associated with the spent nuclear fuel. The oligotrophic and radiologically extreme conditions in this environment do not prevent extensive colonization by microbial communities, which play a defining role in controlling the biogeochemical fate of major radioactive species present. IMPORTANCE Spent nuclear fuel is stored underwater in large ponds prior to processing and disposal. Such environments are intensively radioactive but can be colonized by microorganisms. Colonization of such inhospitable radioactive ponds is surprising, and the survival mechanisms that microbes use is of fundamental interest. It is also important to study these unusual ecosystems, as microbes growing in the pond waters may accumulate radionuclides present in the waters (for bioremediation applications), while high cell loads can hamper management of the ponds due to poor visibility. In this study, an outdoor pond at the U.K. Sellafield facility was colonized by a seasonal bloom of microorganisms, able to accumulate high levels of 137 Cs and 90 Sr and dominated by the alga Haematococcus This organism is not normally associated with deep water bodies, but it can adapt to radioactive environments via the production of the pigment astaxanthin, which protects the cells from radiation damage. Copyright © 2018 MeGraw et al.

Playing by the rules? Phenotypic adaptation to temperate environments in an American marsupial

PubMed Central

Harrigan, Ryan J.; Wayne, Robert K.

2018-01-01

Phenotypic variation along environmental gradients can provide evidence suggesting local adaptation has shaped observed morphological disparities. These differences, in traits such as body and extremity size, as well as skin and coat pigmentation, may affect the overall fitness of individuals in their environments. The Virginia opossum (Didelphis virginiana) is a marsupial that shows phenotypic variation across its range, one that has recently expanded into temperate environments. It is unknown, however, whether the variation observed in the species fits adaptive ecogeographic patterns, or if phenotypic change is associated with any environmental factors. Using phenotypic measurements of over 300 museum specimens of Virginia opossum, collected throughout its distribution range, we applied regression analysis to determine if phenotypes change along a latitudinal gradient. Then, using predictors from remote-sensing databases and a random forest algorithm, we tested environmental models to find the most important variables driving the phenotypic variation. We found that despite the recent expansion into temperate environments, the phenotypic variation in the Virginia opossum follows a latitudinal gradient fitting three adaptive ecogeographic patterns codified under Bergmann’s, Allen’s and Gloger’s rules. Temperature seasonality was an important predictor of body size variation, with larger opossums occurring at high latitudes with more seasonal environments. Annual mean temperature predicted important variation in extremity size, with smaller extremities found in northern populations. Finally, we found that precipitation and temperature seasonality as well as low temperatures were strong environmental predictors of skin and coat pigmentation variation; darker opossums are distributed at low latitudes in warmer environments with higher precipitation seasonality. These results indicate that the adaptive mechanisms underlying the variation in body size, extremity size and pigmentation are related to the resource seasonality, heat conservation, and pathogen-resistance hypotheses, respectively. Our findings suggest that marsupials may be highly susceptible to environmental changes, and in the case of the Virginia opossum, the drastic phenotypic evolution in northern populations may have arisen rapidly, facilitating the colonization of seasonal and colder habitats of temperate North America. PMID:29607255

Impacts of Climate Change On The Occurrence of Extreme Events: The Mice Project

NASA Astrophysics Data System (ADS)

Palutikof, J. P.; Mice Team

It is widely accepted that climate change due to global warming will have substan- tial impacts on the natural environment, and on human activities. Furthermore, it is increasingly recognized that changes in the severity and frequency of extreme events, such as windstorm and flood, are likely to be more important than changes in the average climate. The EU-funded project MICE (Modelling the Impacts of Climate Extremes) commenced in January 2002. It seeks to identify the likely changes in the occurrence of extremes of rainfall, temperature and windstorm due to global warm- ing, using information from climate models as a basis, and to study the impacts of these changes in selected European environments. The objectives are: a) to evaluate, by comparison with gridded and station observations, the ability of climate models to successfully reproduce the occurrence of extremes at the required spatial and temporal scales. b) to analyse model output with respect to future changes in the occurrence of extremes. Statistical analyses will determine changes in (i) the return periods of ex- tremes, (ii) the joint probability of extremes (combinations of damaging events such as windstorm followed by heavy rain), (iii) the sequential behaviour of extremes (whether events are well-separated or clustered) and (iv) the spatial patterns of extreme event occurrence across Europe. The range of uncertainty in model predictions will be ex- plored by analysing changes in model experiments with different spatial resolutions and forcing scenarios. c) to determine the impacts of the predicted changes in extremes occurrence on selected activity sectors: agriculture (Mediterranean drought), commer- cial forestry and natural forest ecosystems (windstorm and flood in northern Europe, fire in the Mediterranean), energy use (temperature extremes), tourism (heat stress and Mediterranean beach holidays, changes in the snow pack and winter sports ) and civil protection/insurance (windstorm and flood). Impacts will be evaluated through a combination of techniques ranging from quantitative analyses through to expert judge- ment. Throughout the project, a continuing dialogue with stakeholders and end-users will be maintained.

Environment Monitor

NASA Technical Reports Server (NTRS)

1988-01-01

Viking landers touched down on Mars equipped with a variety of systems to conduct automated research, each carrying a compact but highly sophisticated instrument for analyzing Martian soil and atmosphere. Instrument called a Gas Chromatography/Mass Spectrometer (GC/MS) had to be small, lightweight, shock resistant, highly automated and extremely sensitive, yet require minimal electrical power. Viking Instruments Corporation commercialized this technology and targeted their primary market as environmental monitoring, especially toxic and hazardous waste site monitoring. Waste sites often contain chemicals in complex mixtures, and the conventional method of site characterization, taking samples on-site and sending them to a laboratory for analysis is time consuming and expensive. Other terrestrial applications are explosive detection in airports, drug detection, industrial air monitoring, medical metabolic monitoring and for military, chemical warfare agents.

Coordinating Chemical and Mineralogical Analyses of Antarctic Dry Valley Sediments as Potential Analogs for Mars

NASA Technical Reports Server (NTRS)

Patel, S. N.; Bishop, J. L.; Englert, P.; Gibson, E. K.

2015-01-01

The Antarctic Dry Valleys (ADV) provide a unique terrestrial analog for Martian surface processes as they are extremely cold and dry sedimentary environments. The surface geology and the chemical composition of the Dry Valleys that are similar to Mars suggest the possible presence of these soil-formation processes on Mars. The soils and sediments from Wright Valley, Antarctica were investigated in this study to examine mineralogical and chemical changes along the surface layer in this region and as a function of depth. Surface samples collected near Prospect Mesa and Don Juan Pond of the ADV were analyzed using visible/near-infrared (VNIR) and mid-IR reflectance spectroscopy and major and trace element abundances.

High temperature x-ray micro-tomography

DOE Office of Scientific and Technical Information (OSTI.GOV)

MacDowell, Alastair A., E-mail: aamacdowell@lbl.gov; Barnard, Harold; Parkinson, Dilworth Y.

2016-07-27

There is increasing demand for 3D micro-scale time-resolved imaging of samples in realistic - and in many cases extreme environments. The data is used to understand material response, validate and refine computational models which, in turn, can be used to reduce development time for new materials and processes. Here we present the results of high temperature experiments carried out at the x-ray micro-tomography beamline 8.3.2 at the Advanced Light Source. The themes involve material failure and processing at temperatures up to 1750°C. The experimental configurations required to achieve the requisite conditions for imaging are described, with examples of ceramic matrixmore » composites, spacecraft ablative heat shields and nuclear reactor core Gilsocarbon graphite.« less

GaAs Quantum Dot Thermometry Using Direct Transport and Charge Sensing

NASA Astrophysics Data System (ADS)

Maradan, D.; Casparis, L.; Liu, T.-M.; Biesinger, D. E. F.; Scheller, C. P.; Zumbühl, D. M.; Zimmerman, J. D.; Gossard, A. C.

2014-06-01

We present measurements of the electron temperature using gate-defined quantum dots formed in a GaAs 2D electron gas in both direct transport and charge sensing mode. Decent agreement with the refrigerator temperature was observed over a broad range of temperatures down to 10 mK. Upon cooling nuclear demagnetization stages integrated into the sample wires below 1 mK, the device electron temperature saturates, remaining close to 10 mK. The extreme sensitivity of the thermometer to its environment as well as electronic noise complicates temperature measurements but could potentially provide further insight into the device characteristics. We discuss thermal coupling mechanisms, address possible reasons for the temperature saturation and delineate the prospects of further reducing the device electron temperature.

Design and Experimental Verification of Chang'E-3 Moon-night Survival Device for APXS

NASA Astrophysics Data System (ADS)

Deng-yi, Chen; Jian, Wu; Yi-ming, Hu; Jin, Chang; Yi-zhong, Gong; Ming-sheng, Cai; Huan-yu, Wang; Jia-yu, Zhang; Xing-zhu, Cui; Jin-zhou, Wang

2016-07-01

The Active Particle X-ray Spectrometer (APXS) is one of the 4 scientific payloads of Chang'E-3 (CE-3) Lunar Rover, of which the scientific object is to identify the elements of lunar soil and rock samples by a carried radioactive source to trigger and detect the characteristic X-ray from them. According to the extreme temperature environment of the APXS and under the restriction of limited resources, this paper presents the design and analysis of the moon-night survival device RHU (radioisotope heating unit) for the APXS, and describes the corresponding environmental tests on its structure dynamics and moon-night survival. Finally, its reinstallation on the launch tower and the preliminary result of its on-orbit operation are introduced.

Effects of virtual reality-based bilateral upper-extremity training on brain activity in post-stroke patients.

PubMed

Lee, Su-Hyun; Kim, Yu-Mi; Lee, Byoung-Hee

2015-07-01

[Purpose] This study investigated the therapeutic effects of virtual reality-based bilateral upper-extremity training on brain activity in patients with stroke. [Subjects and Methods] Eighteen chronic stroke patients were divided into two groups: the virtual reality-based bilateral upper-extremity training group (n = 10) and the bilateral upper-limb training group (n = 8). The virtual reality-based bilateral upper-extremity training group performed bilateral upper-extremity exercises in a virtual reality environment, while the bilateral upper-limb training group performed only bilateral upper-extremity exercise. All training was conducted 30 minutes per day, three times per week for six weeks, followed by brain activity evaluation. [Results] Electroencephalography showed significant increases in concentration in the frontopolar 2 and frontal 4 areas, and significant increases in brain activity in the frontopolar 1 and frontal 3 areas in the virtual reality-based bilateral upper-extremity training group. [Conclusion] Virtual reality-based bilateral upper-extremity training can improve the brain activity of stroke patients. Thus, virtual reality-based bilateral upper-extremity training is feasible and beneficial for improving brain activation in stroke patients.

Extreme Physics

NASA Astrophysics Data System (ADS)

Colvin, Jeff; Larsen, Jon

2013-11-01

Acknowledgements; 1. Extreme environments: what, where, how; 2. Properties of dense and classical plasmas; 3. Laser energy absorption in matter; 4. Hydrodynamic motion; 5. Shocks; 6. Equation of state; 7. Ionization; 8. Thermal energy transport; 9. Radiation energy transport; 10. Magnetohydrodynamics; 11. Considerations for constructing radiation-hydrodynamics computer codes; 12. Numerical simulations; Appendix: units and constants, glossary of symbols; References; Bibliography; Index.

Extremely Selective Attention: Eye-Tracking Studies of the Dynamic Allocation of Attention to Stimulus Features in Categorization

ERIC Educational Resources Information Center

Blair, Mark R.; Watson, Marcus R.; Walshe, R. Calen; Maj, Fillip

2009-01-01

Humans have an extremely flexible ability to categorize regularities in their environment, in part because of attentional systems that allow them to focus on important perceptual information. In formal theories of categorization, attention is typically modeled with weights that selectively bias the processing of stimulus features. These theories…

Influence of oxidation temperature on the oxide scale formation of NiCoCrAl coatings

NASA Astrophysics Data System (ADS)

Sugiarti, E.; Zaini, K. A.; Sundawa, R.; Wang, Y.; Ohnuki, S.; Hayashi, S.

2017-04-01

Intermetalic coatings of NiCoCrAl have been successfully developed on low carbon steel substrate to improve oxidation resistance in extreme environments. The influence of oxidation temperature on the oxide scale formation was studied in the temperature range of 600-1000 °C. The measurements were made in air under isothermal oxidation test for 100 h. The surface morphology showed that a cauliflower like structure developed entire the oxide scale of sample oxidized at 800 °C and 1000 °C, while partly distributed on the surface of sample oxidized at 600 °C. The XRD analysis identified Cr2O3 phase predominantly formed on the oxidized sample at 600 °C and meta-stable Al2O3 with several polymorphs crystalline structures: η, δ, θ, κ, and α-Al2O3 at relatively high temperatures, i.e. 800 °C and 1000 °C. A Cross-sectional microstructure showed that complex and porous structures formed on the top surface of 600 °C and 1000 °C samples. In contrast, a very thin oxide scale formed on 800 °C oxidized samples and it appeared to act as a diffusion barrier of oxygen to diffuse inward, hence could increase in the service life of carbon steel substrate.

Bacterial communities in an ultrapure water containing storage tank of a power plant.

PubMed

Bohus, Veronika; Kéki, Zsuzsa; Márialigeti, Károly; Baranyi, Krisztián; Patek, Gábor; Schunk, János; Tóth, Erika M

2011-12-01

Ultrapure waters (UPWs) containing low levels of organic and inorganic compounds provide extreme environment. On contrary to that microbes occur in such waters and form biofilms on surfaces, thus may induce corrosion processes in many industrial applications. In our study, refined saltless water (UPW) produced for the boiler of a Hungarian power plant was examined before and after storage (sampling the inlet [TKE] and outlet [TKU] waters of a storage tank) with cultivation and culture independent methods. Our results showed increased CFU and direct cell counts after the storage. Cultivation results showed the dominance of aerobic, chemoorganotrophic α-Proteobacteria in both samples. In case of TKU sample, a more complex bacterial community structure could be detected. The applied molecular method (T-RFLP) indicated the presence of a complex microbial community structure with changes in the taxon composition: while in the inlet water sample (TKE) α-Proteobacteria (Sphingomonas sp., Novosphingobium hassiacum) dominated, in the outlet water sample (TKU) the bacterial community shifted towards the dominance of α-Proteobacteria (Rhodoferax sp., Polynucleobacter sp., Sterolibacter sp.), CFB (Bacteroidetes, formerly Cytophaga-Flavobacterium-Bacteroides group) and Firmicutes. This shift to the direction of fermentative communities suggests that storage could help the development of communities with an increased tendency toward corrosion.

Environmental Conditions for Space Flight Hardware: A Survey

NASA Technical Reports Server (NTRS)

Plante, Jeannette; Lee, Brandon

2005-01-01

Interest in generalization of the physical environment experienced by NASA hardware from the natural Earth environment (on the launch pad), man-made environment on Earth (storage acceptance an d qualification testing), the launch environment, and the space environment, is ed to find commonality among our hardware in an effort to reduce cost and complexity. NASA is entering a period of increase in its number of planetary missions and it is important to understand how our qualification requirements will evolve with and track these new environments. Environmental conditions are described for NASA projects in several ways for the different periods of the mission life cycle. At the beginning, the mission manager defines survivability requirements based on the mission length, orbit, launch date, launch vehicle, and other factors . such as the use of reactor engines. Margins are then applied to these values (temperature extremes, vibration extremes, radiation tolerances, etc,) and a new set of conditions is generalized for design requirements. Mission assurance documents will then assign an additional margin for reliability, and a third set of values is provided for during testing. A fourth set of environmental condition values may evolve intermittently from heritage hardware that has been tested to a level beyond the actual mission requirement. These various sets of environment figures can make it quite confusing and difficult to capture common hardware environmental requirements. Environmental requirement information can be found in a wide variety of places. The most obvious is with the individual projects. We can easily get answers to questions about temperature extremes being used and radiation tolerance goals, but it is more difficult to map the answers to the process that created these requirements: for design, for qualification, and for actual environment with no margin applied. Not everyone assigned to a NASA project may have that kind of insight, as many have only the environmental requirement numbers needed to do their jobs but do not necessarily have a programmatic-level understanding of how all of the environmental requirements fit together.

What Is Life? What Was Life? What Will Life Be?

NASA Astrophysics Data System (ADS)

Deamer, D.

Our laboratory is exploring self-assembly processes and polymerization reactions of organic compounds in natural geothermal environments and related laboratory simulations. Although the physical environment that fostered primitive cellular life is still largely unconstrained, we can be reasonably confident that liquid water was required, together with a source of organic compounds and energy to drive polymerization reactions. There must also have been a process by which the compounds were sufficiently concentrated to undergo physical and chemical interactions. In earlier work we observed that macromolecules such as nucleic acids and proteins are readily encapsulated in membranous boundaries during wet-dry cycles such as those that would occur at the edges of geothermal springs or tide pools. The resulting structures are referred to as protocells, in that they exhibit certain properties of living cells and are models of the kinds of encapsulated macromolecular systems that would have led toward the first forms of cellular life. However, the assembly of protocells is markedly inhibited by conditions associated with extreme environments: High temperature, high salt concentrations, and low pH ranges. From a biophysical perspective, it follows that the most plausible planetary environment for the origin of cellular life would be an aqueous phase at moderate temperature ranges and low ionic strength, having a pH value near neutrality and divalent cations at submillimolar concentrations. This suggestion is in marked contrast to the view that life most likely began in a geothermal or marine environment, perhaps even the extreme environment of a hydrothermal vent. A more plausible site for the origin of cellular life would be fresh water pools maintained by rain falling on volcanic land masses resembling present-day Hawaii and Iceland. After the first cellular life was able to establish itself in a relatively benign environment, it would rapidly begin to adapt through Darwinian selection to more rigorous environments, including the extreme temperatures, salt concentrations and pH ranges that we now associate with the limits of life on the Earth.

Countermeasures to Mitigate the Negative Impact of Sensory Deprivation and Social Isolation in Long-Duration Space Flight

NASA Technical Reports Server (NTRS)

Bachman, Katharine Ridgeway OBrien; Otto, Christian; Leveton, Lauren

2012-01-01

Long-duration space flight presents several challenges to the behavioral health of crew members. The environment that they are likely to experience will be isolated, confined, and extreme (ICE) and, as such, crew members will experience extreme sensory deprivation and social isolation. The current paper briefly notes the behavioral, cognitive, and affective consequences of psychological stress induced by ICE environments and proposes nine countermeasures aimed at mitigating the negative effects of sensory deprivation and social isolation. Implementation of countermeasures aims to maintain successful crew performance and psychological well-being in a long-duration space flight mission.

Remotely Powered Reconfigurable Receiver for Extreme Environment Sensing Platforms

NASA Technical Reports Server (NTRS)

Sheldon, Douglas J.

2012-01-01

Wireless sensors connected in a local network offer revolutionary exploration capabilities, but the current solutions do not work in extreme environments of low temperatures (200K) and low to moderate radiation levels (<50 krad). These sensors (temperature, radiation, infrared, etc.) would need to operate outside the spacecraft/ lander and be totally independent of power from the spacecraft/lander. Flash memory field-programmable gate arrays (FPGAs) are being used as the main signal processing and protocol generation platform in a new receiver. Flash-based FPGAs have been shown to have at least 100 reduced standby power and 10 reduction operating power when compared to normal SRAM-based FPGA technology.

Numerical simulation of crystal fractionation in shergottite meteorites

NASA Astrophysics Data System (ADS)

Grimm, R. E.; McSween, H. Y., Jr.

Cumulus clinopyroxenes in the Shergotty and Zagami meteorites suggest crystal fractionation occurred, possibly by gravitative settling. Numerical models of this process in a nonconvecting environment argue that the small phenocrysts can segregate only under extreme conditions of cooling time or gravitational field strength. Since textures indicate that cooling time was not excessive, a large (planetary) g is required by these models, in agreement with other suggestions that the shergottite parent body may be Mars. Other calculations indicate that it is extremely difficult to produce the observed textures in a convecting environment, unless crystal setting occurred in a quiescent zone at the bottom of the magma chamber.

Fiberoptic sensors for rocket engine applications

NASA Technical Reports Server (NTRS)

Ballard, R. O.

1992-01-01

A research effort was completed to summarize and evaluate the current level of technology in fiberoptic sensors for possible applications in integrated control and health monitoring (ICHM) systems in liquid propellant engines. The environment within a rocket engine is particuarly severe with very high temperatures and pressures present combined with extremely rapid fluid and gas flows, and high-velocity and high-intensity acoustc waves. Application of fiberoptic technology to rocket engine health monitoring is a logical evolutionary step in ICHM development and presents a significant challenge. In this extremely harsh environment, the additional flexibility of fiberoptic techniques to augment conventional sensor technologies offer abundant future potential.

Numerical simulation of crystal fractionation in shergottite meteorites

NASA Technical Reports Server (NTRS)

Grimm, R. E.; Mcsween, H. Y., Jr.

1982-01-01

Cumulus clinopyroxenes in the Shergotty and Zagami meteorites suggest crystal fractionation occurred, possibly by gravitative settling. Numerical models of this process in a nonconvecting environment argue that the small phenocrysts can segregate only under extreme conditions of cooling time or gravitational field strength. Since textures indicate that cooling time was not excessive, a large (planetary) g is required by these models, in agreement with other suggestions that the shergottite parent body may be Mars. Other calculations indicate that it is extremely difficult to produce the observed textures in a convecting environment, unless crystal setting occurred in a quiescent zone at the bottom of the magma chamber.

On the response of halophilic archaea to space conditions.

PubMed

Leuko, Stefan; Rettberg, Petra; Pontifex, Ashleigh L; Burns, Brendan P

2014-02-21

Microorganisms are ubiquitous and can be found in almost every habitat and ecological niche on Earth. They thrive and survive in a broad spectrum of environments and adapt to rapidly changing external conditions. It is of great interest to investigate how microbes adapt to different extreme environments and with modern human space travel, we added a new extreme environment: outer space. Within the last 50 years, technology has provided tools for transporting microbial life beyond Earth's protective shield in order to study in situ responses to selected conditions of space. This review will focus on halophilic archaea, as, due to their ability to survive in extremes, they are often considered a model group of organisms to study responses to the harsh conditions associated with space. We discuss ground-based simulations, as well as space experiments, utilizing archaea, examining responses and/or resistance to the effects of microgravity and UV in particular. Several halophilic archaea (e.g., Halorubrum chaoviator) have been exposed to simulated and actual space conditions and their survival has been determined as well as the protective effects of halite shown. Finally, the intriguing potential of archaea to survive on other planets or embedded in a meteorite is postulated.

A walk on the tundra: Host-parasite interactions in an extreme environment.

PubMed

Kutz, Susan J; Hoberg, Eric P; Molnár, Péter K; Dobson, Andy; Verocai, Guilherme G

2014-08-01

Climate change is occurring very rapidly in the Arctic, and the processes that have taken millions of years to evolve in this very extreme environment are now changing on timescales as short as decades. These changes are dramatic, subtle and non-linear. In this article, we discuss the evolving insights into host-parasite interactions for wild ungulate species, specifically, muskoxen and caribou, in the North American Arctic. These interactions occur in an environment that is characterized by extremes in temperature, high seasonality, and low host species abundance and diversity. We believe that lessons learned in this system can guide wildlife management and conservation throughout the Arctic, and can also be generalized to more broadly understand host-parasite interactions elsewhere. We specifically examine the impacts of climate change on host-parasite interactions and focus on: (I) the direct temperature effects on parasites; (II) the importance of considering the intricacies of host and parasite ecology for anticipating climate change impacts; and (III) the effect of shifting ecological barriers and corridors. Insights gained from studying the history and ecology of host-parasite systems in the Arctic will be central to understanding the role that climate change is playing in these more complex systems.

A walk on the tundra: Host–parasite interactions in an extreme environment

PubMed Central

Kutz, Susan J.; Hoberg, Eric P.; Molnár, Péter K.; Dobson, Andy; Verocai, Guilherme G.

2014-01-01

Climate change is occurring very rapidly in the Arctic, and the processes that have taken millions of years to evolve in this very extreme environment are now changing on timescales as short as decades. These changes are dramatic, subtle and non-linear. In this article, we discuss the evolving insights into host–parasite interactions for wild ungulate species, specifically, muskoxen and caribou, in the North American Arctic. These interactions occur in an environment that is characterized by extremes in temperature, high seasonality, and low host species abundance and diversity. We believe that lessons learned in this system can guide wildlife management and conservation throughout the Arctic, and can also be generalized to more broadly understand host–parasite interactions elsewhere. We specifically examine the impacts of climate change on host–parasite interactions and focus on: (I) the direct temperature effects on parasites; (II) the importance of considering the intricacies of host and parasite ecology for anticipating climate change impacts; and (III) the effect of shifting ecological barriers and corridors. Insights gained from studying the history and ecology of host–parasite systems in the Arctic will be central to understanding the role that climate change is playing in these more complex systems. PMID:25180164

Mean-field potential approach for thermodynamic properties of lanthanide: Europium as a prototype

NASA Astrophysics Data System (ADS)

Kumar, Priyank; Bhatt, N. K.; Vyas, P. R.; Gohel, V. B.

2018-03-01

In the present paper, a simple conjunction scheme [mean-field potential (MFP) + local pseudopotential] is used to study the thermodynamic properties of divalent lanthanide europium (Eu) at extreme environment. Present study has been carried out due to the fact that divalent nature of Eu arises because of stable half-filled 4f-shell at ambient condition, which has great influence on the thermodynamic properties at extreme environment. Due to such electronic structure, it is different from remaining lanthanides having incomplete 4f-shell. The presently computed results of thermodynamic properties of Eu are in good agreement with the experimental results. Looking to such success, it seems that the concept of MFP approach is successful to account contribution due to nuclear motion to the total Helmholtz free energy at finite temperatures and pressure-induced inter-band transfer of electrons for condensed state of matter. The local pseudopotential is used to evaluate cold energy and hence MFP accounts the s-p-d-f hybridization properly. Looking to the reliability and transferability along with its computational and conceptual simplicity, we would like to extend the present scheme for the study of thermodynamic properties of remaining lanthanides and actinides at extreme environment.

Superior radiation-resistant nanoengineered austenitic 304L stainless steel for applications in extreme radiation environments

NASA Astrophysics Data System (ADS)

Sun, C.; Zheng, S.; Wei, C. C.; Wu, Y.; Shao, L.; Yang, Y.; Hartwig, K. T.; Maloy, S. A.; Zinkle, S. J.; Allen, T. R.; Wang, H.; Zhang, X.

2015-01-01

Nuclear energy provides more than 10% of electrical power internationally, and the increasing engagement of nuclear energy is essential to meet the rapid worldwide increase in energy demand. A paramount challenge in the development of advanced nuclear reactors is the discovery of advanced structural materials that can endure extreme environments, such as severe neutron irradiation damage at high temperatures. It has been known for decades that high dose radiation can introduce significant void swelling accompanied by precipitation in austenitic stainless steel (SS). Here we report, however, that through nanoengineering, ultra-fine grained (UFG) 304L SS with an average grain size of ~100 nm, can withstand Fe ion irradiation at 500°C to 80 displacements-per-atom (dpa) with moderate grain coarsening. Compared to coarse grained (CG) counterparts, swelling resistance of UFG SS is improved by nearly an order of magnitude and swelling rate is reduced by a factor of 5. M23C6 precipitates, abundant in irradiated CG SS, are largely absent in UFG SS. This study provides a nanoengineering approach to design and discover radiation tolerant metallic materials for applications in extreme radiation environments.

Ionic Strength Is a Barrier to the Habitability of Mars.

PubMed

Fox-Powell, Mark G; Hallsworth, John E; Cousins, Claire R; Cockell, Charles S

2016-06-01

The thermodynamic availability of water (water activity) strictly limits microbial propagation on Earth, particularly in hypersaline environments. A considerable body of evidence indicates the existence of hypersaline surface waters throughout the history of Mars; therefore it is assumed that, as on Earth, water activity is a major limiting factor for martian habitability. However, the differing geological histories of Earth and Mars have driven variations in their respective aqueous geochemistry, with as-yet-unknown implications for habitability. Using a microbial community enrichment approach, we investigated microbial habitability for a suite of simulated martian brines. While the habitability of some martian brines was consistent with predictions made from water activity, others were uninhabitable even when the water activity was biologically permissive. We demonstrate experimentally that high ionic strength, driven to extremes on Mars by the ubiquitous occurrence of multivalent ions, renders these environments uninhabitable despite the presence of biologically available water. These findings show how the respective geological histories of Earth and Mars, which have produced differences in the planets' dominant water chemistries, have resulted in different physicochemical extremes which define the boundary space for microbial habitability. Habitability-Mars-Salts-Water activity-Life in extreme environments. Astrobiology 16, 427-442.

Superior radiation-resistant nanoengineered austenitic 304L stainless steel for applications in extreme radiation environments

DOE PAGES

Sun, C.; Zheng, S.; Wei, C. C.; ...

2015-01-15

Nuclear energy provides more than 10% of electrical power internationally, and the increasing engagement of nuclear energy is essential to meet the rapid worldwide increase in energy demand. A paramount challenge in the development of advanced nuclear reactors is the discovery of advanced structural materials that can endure extreme environments, such as severe neutron irradiation damage at high temperatures. It has been known for decades that high dose radiation can introduce significant void swelling accompanied by precipitation in austenitic stainless steel (SS). Here we report, however, that through nanoengineering, ultra-fine grained (UFG) 304L SS with an average grain size ofmore » ~100 nm, can withstand Fe ion irradiation at 500°C to 80 displacements-per-atom (dpa) with moderate grain coarsening. Compared to coarse grained (CG) counterparts, swelling resistance of UFG SS is improved by nearly an order of magnitude and swelling rate is reduced by a factor of 5. M₂₃C₆ precipitates, abundant in irradiated CG SS, are largely absent in UFG SS. This study provides a nanoengineering approach to design and discover radiation tolerant metallic materials for applications in extreme radiation environments.« less