The logic of communication: roles for mobile transcription factors in plants.

PubMed

Long, Yuchen; Scheres, Ben; Blilou, Ikram

2015-02-01

Mobile transcription factors play many roles in plant development. Here, we compare the use of mobile transcription factors as signals with some canonical signal transduction processes in prokaryotes and eukaryotes. After an initial survey, we focus on the SHORT-ROOT pathway in Arabidopsis roots to show that, despite the simplicity of the concept of mobile transcription factor signalling, many lines of evidence reveal a surprising complexity in control mechanisms linked to this process. We argue that these controls bestow precision, robustness, and versatility on mobile transcription factor signalling. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Wind Power Plant SCADA and Controls

DOE Office of Scientific and Technical Information (OSTI.GOV)

Badrzadeh, Babak; Castillo, Nestor; Bradt, M.

2011-01-01

Modern Wind Power Plants (WPPs) contain a variety of intelligent electronic devices (IEDs), Supervisory Control and Data Acquisition (SCADA) and communication systems. This paper discusses the issues related to a typical WPP's SCADA and Control. Presentation topics are: (1) Wind Turbine Controls; (2) Wind Plant SCADA, OEM SCADA Solutions, Third-Party SCADA Solutions; (3) Wind Plant Control; and (4) Security and Reliability Compliance.

Anticipatory control: A software retrofit for current plant controllers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Parthasarathy, S.; Parlos, A.G.; Atiya, A.F.

1993-01-01

The design and simulated testing of an artificial neural network (ANN)-based self-adapting controller for complex process systems are presented in this paper. The proposed controller employs concepts based on anticipatory systems, which have been widely used in the petroleum and chemical industries, and they are slowly finding their way into the power industry. In particular, model predictive control (MPC) is used for the systematic adaptation of the controller parameters to achieve desirable plant performance over the entire operating envelope. The versatile anticipatory control algorithm developed in this study is projected to enhance plant performance and lend robustness to drifts inmore » plant parameters and to modeling uncertainties. This novel technique of integrating recurrent ANNs with a conventional controller structure appears capable of controlling complex, nonlinear, and nonminimum phase process systems. The direct, on-line adaptive control algorithm presented in this paper considers the plant response over a finite time horizon, diminishing the need for manual control or process interruption for controller gain tuning.« less

Biotechnological application of functional genomics towards plant-parasitic nematode control.

PubMed

Li, Jiarui; Todd, Timothy C; Lee, Junghoon; Trick, Harold N

2011-12-01

Plant-parasitic nematodes are primary biotic factors limiting the crop production. Current nematode control strategies include nematicides, crop rotation and resistant cultivars, but each has serious limitations. RNA interference (RNAi) represents a major breakthrough in the application of functional genomics for plant-parasitic nematode control. RNAi-induced suppression of numerous genes essential for nematode development, reproduction or parasitism has been demonstrated, highlighting the considerable potential for using this strategy to control damaging pest populations. In an effort to find more suitable and effective gene targets for silencing, researchers are employing functional genomics methodologies, including genome sequencing and transcriptome profiling. Microarrays have been used for studying the interactions between nematodes and plant roots and to measure both plants and nematodes transcripts. Furthermore, laser capture microdissection has been applied for the precise dissection of nematode feeding sites (syncytia) to allow the study of gene expression specifically in syncytia. In the near future, small RNA sequencing techniques will provide more direct information for elucidating small RNA regulatory mechanisms in plants and specific gene silencing using artificial microRNAs should further improve the potential of targeted gene silencing as a strategy for nematode management. © 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.

Design Of Feedforward Controllers For Multivariable Plants

NASA Technical Reports Server (NTRS)

Seraji, Homayoun

1989-01-01

Controllers based on simple low-order transfer functions. Mathematical criteria derived for design of feedforward controllers for class of multiple-input/multiple-output linear plants. Represented by simple low-order transfer functions, obtained without reconstruction of states of commands and disturbances. Enables plant to track command while remaining unresponsive to disturbance in steady state. Feedback controller added independently to stabilize plant or to make control system less susceptible to variations in parameters of plant.

A Business Case for Nuclear Plant Control Room Modernization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomas, Ken; Lawrie, Sean; Niedermuller, Josef M.

This paper presents a generic business case for implementation of technology that supports Control Room Modernization (CRM). The analysis presented in two forms; 1) a standalone technology upgrade, and 2) a technology upgrade that is built upon and incremental to a prior business case created for Mobile Work Packages (MWP). The business case contends that advanced communication and networking and analytical technologies will allow NPP to conduct control room operations with improved focus by reducing human factors and redundant manpower, and therefore operate with fewer errors. While some labor savings can be harvested in terms of overtime, the majority ofmore » savings are demonstrated as reduced time to take the plant off line and bring back on line in support of outages. The benefits are quantified to a rough order of magnitude that provides directional guidance to NPPs that are interested in developing a similar business case. This business case focuses on modernization of the operator control room and does not consider a complete overhaul and modernization of a plants instrument and control systems. While operators may be considering such an investment at their plants, the sizable capital investment required is not likely supported by a cost/benefit analysis alone. More likely, it is driven by obsolescence and reliability issues, and requires consideration of mechanical condition of plant systems, capital depreciation, financing, relicensing and overall viability of the plant asset over a 20-year horizon in a competitive market. Prior studies [REF] have indicated that such a modernization of plant I&C systems, alone or as part of a larger modernization effort, can yield very significant reductions in O&M costs. However, the depth of research and analysis required to develop a meaningful business case for a plant modernization effort is well beyond the scope of this study. While CRM as considered in this study can be easily integrated as part of grander plant

Aquatic Plants and their Control.

ERIC Educational Resources Information Center

Michigan State Dept. of Natural Resources, Lansing.

Aquatic plants can be divided into two types: algae and macrophytes. The goal of aquatic plant management is to maintain a proper balance of plants within a lake and still retain the lake's recreational and economic importance. Aquatic plant management programs have two phases: long-term management (nutrient control), and short-term management…

Plant MYB Transcription Factors: Their Role in Drought Response Mechanisms

PubMed Central

Baldoni, Elena; Genga, Annamaria; Cominelli, Eleonora

2015-01-01

Water scarcity is one of the major causes of poor plant performance and limited crop yields worldwide and it is the single most common cause of severe food shortage in developing countries. Several molecular networks involved in stress perception, signal transduction and stress responses in plants have been elucidated so far. Transcription factors are major players in water stress signaling. In recent years, different MYB transcription factors, mainly in Arabidopsis thaliana (L.) Heynh. but also in some crops, have been characterized for their involvement in drought response. For some of them there is evidence supporting a specific role in response to water stress, such as the regulation of stomatal movement, the control of suberin and cuticular waxes synthesis and the regulation of flower development. Moreover, some of these genes have also been characterized for their involvement in other abiotic or biotic stresses, an important feature considering that in nature, plants are often simultaneously subjected to multiple rather than single environmental perturbations. This review summarizes recent studies highlighting the role of the MYB family of transcription factors in the adaptive responses to drought stress. The practical application value of MYBs in crop improvement, such as stress tolerance engineering, is also discussed. PMID:26184177

Plant MYB Transcription Factors: Their Role in Drought Response Mechanisms.

PubMed

Baldoni, Elena; Genga, Annamaria; Cominelli, Eleonora

2015-07-13

Water scarcity is one of the major causes of poor plant performance and limited crop yields worldwide and it is the single most common cause of severe food shortage in developing countries. Several molecular networks involved in stress perception, signal transduction and stress responses in plants have been elucidated so far. Transcription factors are major players in water stress signaling. In recent years, different MYB transcription factors, mainly in Arabidopsis thaliana (L.) Heynh. but also in some crops, have been characterized for their involvement in drought response. For some of them there is evidence supporting a specific role in response to water stress, such as the regulation of stomatal movement, the control of suberin and cuticular waxes synthesis and the regulation of flower development. Moreover, some of these genes have also been characterized for their involvement in other abiotic or biotic stresses, an important feature considering that in nature, plants are often simultaneously subjected to multiple rather than single environmental perturbations. This review summarizes recent studies highlighting the role of the MYB family of transcription factors in the adaptive responses to drought stress. The practical application value of MYBs in crop improvement, such as stress tolerance engineering, is also discussed.

Chemical Control of Plant Growth.

ERIC Educational Resources Information Center

Agricultural Research Center (USDA), Beltsville, MD.

Seven experiments are presented in this Science Study Aid to help students investigate the control of plant growth with chemicals. Plant growth regulators, weed control, and chemical pruning are the topics studied in the experiments which are based on investigations that have been and are being conducted at the U. S. Agricultural Research Center,…

A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium

PubMed Central

Hoshi, Ayaka; Oshima, Kenro; Kakizawa, Shigeyuki; Ishii, Yoshiko; Ozeki, Johji; Hashimoto, Masayoshi; Komatsu, Ken; Kagiwada, Satoshi; Yamaji, Yasuyuki; Namba, Shigetou

2009-01-01

One of the most important themes in agricultural science is the identification of virulence factors involved in plant disease. Here, we show that a single virulence factor, tengu-su inducer (TENGU), induces witches' broom and dwarfism and is a small secreted protein of the plant-pathogenic bacterium, phytoplasma. When tengu was expressed in Nicotiana benthamiana plants, these plants showed symptoms of witches' broom and dwarfism, which are typical of phytoplasma infection. Transgenic Arabidopsis thaliana lines expressing tengu exhibited similar symptoms, confirming the effects of tengu expression on plants. Although the localization of phytoplasma was restricted to the phloem, TENGU protein was detected in apical buds by immunohistochemical analysis, suggesting that TENGU was transported from the phloem to other cells. Microarray analyses showed that auxin-responsive genes were significantly down-regulated in the tengu-transgenic plants compared with GUS-transgenic control plants. These results suggest that TENGU inhibits auxin-related pathways, thereby affecting plant development. PMID:19329488

Important biological factors for utilizing native plant species

Treesearch

Loren E. Wiesner

1999-01-01

Native plant species are valuable resources for revegetation of disturbed ecosystems. The success of these plantings is dependent on the native species selected, quality of seed used, condition of the soil, environmental conditions before and after planting, planting equipment used, time of planting, and other factors. Most native species contain dormant seed. Dormancy...

Implications of the degree of controllability of controlled plants in the sense of LQR optimal control

NASA Astrophysics Data System (ADS)

Xia, Yaping; Yin, Minghui; Zou, Yun

2018-01-01

In this paper, the relationship between the degree of controllability (DOC) of controlled plants and the corresponding quadratic optimal performance index in LQR control is investigated for the electro-hydraulic synchronising servo control systems and wind turbine systems, respectively. It is shown that for these two types of systems, the higher the DOC of a controlled plant is, the better the quadratic optimal performance index is. It implies that in some LQR controller designs, the measure of the DOC of a controlled plant can be used as an index for the optimisation of adjustable plant parameters, by which the plant can be controlled more effectively.

Advanced nuclear plant control complex

DOEpatents

Scarola, Kenneth; Jamison, David S.; Manazir, Richard M.; Rescorl, Robert L.; Harmon, Daryl L.

1993-01-01

An advanced control room complex for a nuclear power plant, including a discrete indicator and alarm system (72) which is nuclear qualified for rapid response to changes in plant parameters and a component control system (64) which together provide a discrete monitoring and control capability at a panel (14-22, 26, 28) in the control room (10). A separate data processing system (70), which need not be nuclear qualified, provides integrated and overview information to the control room and to each panel, through CRTs (84) and a large, overhead integrated process status overview board (24). The discrete indicator and alarm system (72) and the data processing system (70) receive inputs from common plant sensors and validate the sensor outputs to arrive at a representative value of the parameter for use by the operator during both normal and accident conditions, thereby avoiding the need for him to assimilate data from each sensor individually. The integrated process status board (24) is at the apex of an information hierarchy that extends through four levels and provides access at each panel to the full display hierarchy. The control room panels are preferably of a modular construction, permitting the definition of inputs and outputs, the man machine interface, and the plant specific algorithms, to proceed in parallel with the fabrication of the panels, the installation of the equipment and the generic testing thereof.

Application of multivariable statistical techniques in plant-wide WWTP control strategies analysis.

PubMed

Flores, X; Comas, J; Roda, I R; Jiménez, L; Gernaey, K V

2007-01-01

The main objective of this paper is to present the application of selected multivariable statistical techniques in plant-wide wastewater treatment plant (WWTP) control strategies analysis. In this study, cluster analysis (CA), principal component analysis/factor analysis (PCA/FA) and discriminant analysis (DA) are applied to the evaluation matrix data set obtained by simulation of several control strategies applied to the plant-wide IWA Benchmark Simulation Model No 2 (BSM2). These techniques allow i) to determine natural groups or clusters of control strategies with a similar behaviour, ii) to find and interpret hidden, complex and casual relation features in the data set and iii) to identify important discriminant variables within the groups found by the cluster analysis. This study illustrates the usefulness of multivariable statistical techniques for both analysis and interpretation of the complex multicriteria data sets and allows an improved use of information for effective evaluation of control strategies.

Plant centromeres: structure and control.

PubMed

Richards, E J; Dawe, R K

1998-04-01

Recent work has led to a better understanding of the molecular components of plant centromeres. Conservation of at least some centromere protein constituents between plant and non-plant systems has been demonstrated. The identity and organization of plant centromeric DNA sequences are also beginning to yield to analysis. While there is little primary DNA sequence conservation among the characterized plant centromeres and their non-plant counterparts, some parallels in centromere genomic organisation can be seen across species. Finally, the emerging idea that centromere activity is controlled epigenetically finds support in an examination of the plant centromere literature.

Redox control of plant growth and development.

PubMed

Kocsy, Gábor; Tari, Irma; Vanková, Radomíra; Zechmann, Bernd; Gulyás, Zsolt; Poór, Péter; Galiba, Gábor

2013-10-01

Redox changes determined by genetic and environmental factors display well-organized interactions in the control of plant growth and development. Diurnal and seasonal changes in the environmental conditions are important for the normal course of these physiological processes and, similarly to their mild irregular alterations, for stress adaptation. However, fast or large-scale environmental changes may lead to damage or death of sensitive plants. The spatial and temporal redox changes influence growth and development due to the reprogramming of metabolism. In this process reactive oxygen and nitrogen species and antioxidants are involved as components of signalling networks. The control of growth, development and flowering by reactive oxygen and nitrogen species and antioxidants in interaction with hormones at organ, tissue, cellular and subcellular level will be discussed in the present review. Unsolved problems of the field, among others the need for identification of new components and interactions in the redox regulatory network at various organization levels using systems biology approaches will be also indicated. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Controllability analysis and decentralized control of a wet limestone flue gas desulfurization plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Perales, A.L.V.; Ortiz, F.J.G.; Ollero, P.

2008-12-15

Presently, decentralized feedback control is the only control strategy used in wet limestone flue gas desulfurization (WLFGD) plants. Proper tuning of this control strategy is becoming an important issue in WLFGD plants because more stringent SO{sub 2} regulations have come into force recently. Controllability analysis is a highly valuable tool for proper design of control systems, but it has not been applied to WLFGD plants so far. In this paper a decentralized control strategy is designed and applied to a WLFGD pilot plant taking into account the conclusions of a controllability analysis. The results reveal that good SO{sub 2} controlmore » in WLFGD plants can be achieved mainly because the main disturbance of the process is well-aligned with the plant and interactions between control loops are beneficial to SO{sub 2} control.« less

Chapter 10. Herbicides for plant control

Treesearch

John F. Vallentine

2004-01-01

Herbicides can be an effective, necessary, and environmentally sound tool for the control of weeds and brush on rangelands (Young and others 1981b). As a result, chemical control is a widely used means of removing unwanted or noxious plants from range and other pasture lands. Selective plant control by mechanical, biological, fire, or manual means should also be...

Microbial Hub Taxa Link Host and Abiotic Factors to Plant Microbiome Variation

PubMed Central

Agler, Matthew T.; Ruhe, Jonas; Kroll, Samuel; Morhenn, Constanze; Kim, Sang-Tae; Weigel, Detlef; Kemen, Eric M.

2016-01-01

Plant-associated microorganisms have been shown to critically affect host physiology and performance, suggesting that evolution and ecology of plants and animals can only be understood in a holobiont (host and its associated organisms) context. Host-associated microbial community structures are affected by abiotic and host factors, and increased attention is given to the role of the microbiome in interactions such as pathogen inhibition. However, little is known about how these factors act on the microbial community, and especially what role microbe–microbe interaction dynamics play. We have begun to address this knowledge gap for phyllosphere microbiomes of plants by simultaneously studying three major groups of Arabidopsis thaliana symbionts (bacteria, fungi and oomycetes) using a systems biology approach. We evaluated multiple potential factors of microbial community control: we sampled various wild A. thaliana populations at different times, performed field plantings with different host genotypes, and implemented successive host colonization experiments under lab conditions where abiotic factors, host genotype, and pathogen colonization was manipulated. Our results indicate that both abiotic factors and host genotype interact to affect plant colonization by all three groups of microbes. Considering microbe–microbe interactions, however, uncovered a network of interkingdom interactions with significant contributions to community structure. As in other scale-free networks, a small number of taxa, which we call microbial “hubs,” are strongly interconnected and have a severe effect on communities. By documenting these microbe–microbe interactions, we uncover an important mechanism explaining how abiotic factors and host genotypic signatures control microbial communities. In short, they act directly on “hub” microbes, which, via microbe–microbe interactions, transmit the effects to the microbial community. We analyzed two “hub” microbes (the

Biological control of invasive plant species: a reassessment for the Anthropocene.

PubMed

Seastedt, Timothy R

2015-01-01

The science of finding, testing and releasing herbivores and pathogens to control invasive plant species has achieved a level of maturity and success that argues for continued and expanded use of this program. The practice, however, remains unpopular with some conservationists, invasion biologists, and stakeholders. The ecological and economic benefits of controlling densities of problematic plant species using biological control agents can be quantified, but the risks and net benefits of biological control programs are often derived from social or cultural rather than scientific criteria. Management of invasive plants is a 'wicked problem', and local outcomes to wicked problems have both positive and negative consequences differentially affecting various groups of stakeholders. The program has inherent uncertainties; inserting species into communities that are experiencing directional or even transformational changes can produce multiple outcomes due to context-specific factors that are further confounded by environmental change drivers. Despite these uncertainties, biological control could play a larger role in mitigation and adaptation strategies used to maintain biological diversity as well as contribute to human well-being by protecting food and fiber resources. © 2014 The Author New Phytologist © 2014 New Phytologist Trust.

Digital Full-Scope Simulation of a Conventional Nuclear Power Plant Control Room, Phase 2: Installation of a Reconfigurable Simulator to Support Nuclear Plant Sustainability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ronald L. Boring; Vivek Agarwal; Kirk Fitzgerald

2013-03-01

The U.S. Department of Energy’s Light Water Reactor Sustainability program has developed a control room simulator in support of control room modernization at nuclear power plants in the U.S. This report highlights the recent completion of this reconfigurable, full-scale, full-scope control room simulator buildout at the Idaho National Laboratory. The simulator is fully reconfigurable, meaning it supports multiple plant models developed by different simulator vendors. The simulator is full-scale, using glasstop virtual panels to display the analog control boards found at current plants. The present installation features 15 glasstop panels, uniquely achieving a complete control room representation. The simulator ismore » also full-scope, meaning it uses the same plant models used for training simulators at actual plants. Unlike in the plant training simulators, the deployment on glasstop panels allows a high degree of customization of the panels, allowing the simulator to be used for research on the design of new digital control systems for control room modernization. This report includes separate sections discussing the glasstop panels, their layout to mimic control rooms at actual plants, technical details on creating a multi-plant and multi-vendor reconfigurable simulator, and current efforts to support control room modernization at U.S. utilities. The glasstop simulator provides an ideal testbed for prototyping and validating new control room concepts. Equally importantly, it is helping create a standardized and vetted human factors engineering process that can be used across the nuclear industry to ensure control room upgrades maintain and even improve current reliability and safety.« less

Biological Control of Aquatic Plants with Pathogenic Fungi

DTIC Science & Technology

1981-01-01

reverse side II necoosary and Ident•l•y by block number) Aquatic plant control Fungi Aquatic plants Pathogenic fungi Biological control Waterhyacinths...BACTERIA ............. ................. D1 2 1 BIOTIGICAL CONTROL OF AQUATIC PLANTS WITH PATHOGENIC FUNGI PART I: INTRODUCTION 1. Plant pathogens have...first noted in Florida. 13. In December of 1973, Dr. K. E. Ctnway isolated a Cercospora species, along with many other fungi , from declining

Add Control: plant virtualization for control solutions in WWTP.

PubMed

Maiza, M; Bengoechea, A; Grau, P; De Keyser, W; Nopens, I; Brockmann, D; Steyer, J P; Claeys, F; Urchegui, G; Fernández, O; Ayesa, E

2013-01-01

This paper summarizes part of the research work carried out in the Add Control project, which proposes an extension of the wastewater treatment plant (WWTP) models and modelling architectures used in traditional WWTP simulation tools, addressing, in addition to the classical mass transformations (transport, physico-chemical phenomena, biological reactions), all the instrumentation, actuation and automation & control components (sensors, actuators, controllers), considering their real behaviour (signal delays, noise, failures and power consumption of actuators). Its ultimate objective is to allow a rapid transition from the simulation of the control strategy to its implementation at full-scale plants. Thus, this paper presents the application of the Add Control simulation platform for the design and implementation of new control strategies at the WWTP of Mekolalde.

Performance test of nutrient control equipment for hydroponic plants

NASA Astrophysics Data System (ADS)

Rahman, Nurhaidar; Kuala, S. I.; Tribowo, R. I.; Anggara, C. E. W.; Susanti, N. D.

2017-11-01

Automatic control equipment has been made for the nutrient content in irrigation water for hydroponic plants. Automatic control equipment with CCT53200E conductivity controller to nutrient content in irrigation water for hydroponic plants, can be used to control the amount of TDS of nutrient solution in the range of TDS numbers that can be set according to the range of TDS requirements for the growth of hydroponically cultivated crops. This equipment can minimize the work time of hydroponic crop cultivators. The equipment measurement range is set between 1260 ppm up to 1610 ppm for spinach plants. Caisim plants were included in this experiment along with spinach plants with a spinach plants TDS range. The average of TDS device is 1450 ppm, while manual (conventional) is 1610 ppm. Nutrient solution in TDS controller has pH 5,5 and temperature 29,2 °C, while manual is pH 5,6 and temperature 31,3 °C. Manually treatment to hydroponic plant crop, yields in an average of 39.6 grams/plant, greater than the yield of spinach plants with TDS control equipment, which is in an average of 24.6 grams / plant. The yield of caisim plants by manual treatment is in an average of 32.3 grams/crop, less than caisim crop yields with TDS control equipment, which is in an average of 49.4 grams/plant.

Adaptation of primocane fruiting raspberry plants to environmental factors under the influence of Bacillus strains in Western Siberia.

PubMed

Belyaev, Anatoly A; Shternshis, Margarita V; Chechenina, Nina S; Shpatova, Tatyana V; Lelyak, Anastasya A

2017-03-01

In geographical locations with a short vegetative season and continental climate that include Western Siberia, growing primocane fruiting raspberry varieties becomes very important. However, it is necessary to help the plants to overcome the environmental stress factors. This study aimed to evaluate the impact of the pre-planting treatment of primocane fruiting raspberry root system with Bacillus strains on the following plant development under variable environmental conditions. In 2012, Bacillus subtilis RCAM В-10641, Bacillus amyloliquefaciens RCAM В-10642, and Bacillus licheniformis RCAM В-10562 were used for inoculating the root system of primocane fruiting raspberry cultivar Nedosyagaemaya before planting. The test suspensions were 10 5  CFU/ml for each bacterial strains. The effects of this treatment on plant growth and crop productivity were estimated in 2012-2015 growing seasons differed by environmental conditions. The pre-planting treatment by the bacterial strains increased the number of new raspberry canes and the number of plant generative organs as well as crop productivity compared to control. In addition, these bacilli acted as the standard humic fertilizer. Variable environmental factors such as air temperature, relative humidity, and winter and spring frosts seriously influenced the plant biological parameters and crop productivity of control plants. At the same time, the pre-planting primocane fruiting root treatment by Bacillus strains decreased the negative effects of abiotic stresses on plants in all years of the research. Of the three strains studied, B. subtilis was shown to reveal the best results in adaptation of primocane fruiting raspberry plants to environmental factors in Western Siberia. For the first time, the role of Bacillus strains in enhancing frost resistance in primocane fruiting raspberry plants was shown. These bacilli are capable of being the basis of multifunctional biological formulations for effective plant and

Advanced multivariable control of a turboexpander plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Altena, D.; Howard, M.; Bullin, K.

1998-12-31

This paper describes an application of advanced multivariable control on a natural gas plant and compares its performance to the previous conventional feed-back control. This control algorithm utilizes simple models from existing plant data and/or plant tests to hold the process at the desired operating point in the presence of disturbances and changes in operating conditions. The control software is able to accomplish this due to effective handling of process variable interaction, constraint avoidance and feed-forward of measured disturbances. The economic benefit of improved control lies in operating closer to the process constraints while avoiding significant violations. The South Texasmore » facility where this controller was implemented experienced reduced variability in process conditions which increased liquids recovery because the plant was able to operate much closer to the customer specified impurity constraint. An additional benefit of this implementation of multivariable control is the ability to set performance criteria beyond simple setpoints, including process variable constraints, relative variable merit and optimizing use of manipulated variables. The paper also details the control scheme applied to the complex turboexpander process and some of the safety features included to improve reliability.« less

Advanced nuclear plant control room complex

DOEpatents

Scarola, Kenneth; Jamison, David S.; Manazir, Richard M.; Rescorl, Robert L.; Harmon, Daryl L.

1993-01-01

An advanced control room complex for a nuclear power plant, including a discrete indicator and alarm system (72) which is nuclear qualified for rapid response to changes in plant parameters and a component control system (64) which together provide a discrete monitoring and control capability at a panel (14-22, 26, 28) in the control room (10). A separate data processing system (70), which need not be nuclear qualified, provides integrated and overview information to the control room and to each panel, through CRTs (84) and a large, overhead integrated process status overview board (24). The discrete indicator and alarm system (72) and the data processing system (70) receive inputs from common plant sensors and validate the sensor outputs to arrive at a representative value of the parameter for use by the operator during both normal and accident conditions, thereby avoiding the need for him to assimilate data from each sensor individually. The integrated process status board (24) is at the apex of an information hierarchy that extends through four levels and provides access at each panel to the full display hierarchy. The control room panels are preferably of a modular construction, permitting the definition of inputs and outputs, the man machine interface, and the plant specific algorithms, to proceed in parallel with the fabrication of the panels, the installation of the equipment and the generic testing thereof.

Human factors engineering verification and validation for APR1400 computerized control room

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shin, Y. C.; Moon, H. K.; Kim, J. H.

2006-07-01

This paper introduces the Advanced Power Reactor 1400 (APR1400) HFE V and V activities the Korea Hydro Nuclear Plant Co. LTD. (KHNP) has performed for the last 10 years and some of the lessons learned through these activities. The features of APR1400 main control room include large display panel, redundant compact workstations, computer-based procedure, and safety console. Several iterations of human factors evaluations have been performed from small scale proof of concept tests to large scale integrated system tests for identifying human engineering deficiencies in the human system interface design. Evaluations in the proof of concept test were focused onmore » checking the presence of any show stopper problems in the design concept. Later evaluations were mostly for finding design problems and for assuring the resolution of human factors issues of advanced control room. The results of design evaluations were useful not only for refining the control room design, but also for licensing the standard design. Several versions of APR1400 mock-ups with dynamic simulation models of currently operating Korea Standard Nuclear Plant (KSNP) have been used for the evaluations with the participation of operators from KSNP plants. (authors)« less

Soil and plant factors influencing the accumulation of heavy metals by plants.

PubMed Central

Cataldo, D A; Wildung, R E

1978-01-01

The use of plants to monitor heavy metal pollution in the terrestrial environment must be based on a cognizance of the complicated, integrated effects of pollutant source and soil-plant variables. To be detectable in plants, pollutant sources must significantly increase the plant available metal concentration in soil. The major factor governing metal availability to plants in soils is the solubility of the metal associated with the solid phase, since in order for root uptake to occur, a soluble species must exist adjacent to the root membrane for some finite period. The rate of release and form of this soluble species will have a strong influence on the rate and extent of uptake and, perhaps, mobility and toxicity in the plant and consuming animals. The factors influencing solubility and form of available metal species in soil vary widely geographically and include the concentration and chemical form of the element entering soil, soil properties (endogenous metal concentration, mineralogy, particle size distribution), and soil processes (e.g., mineral weathering, microbial activity), as these influence the kinetics of sorption reactions, metal concentration in solution and the form of soluble and insoluble chemical species. The plant root represents the first barrier to the selective accumulation of ions present in soil solution. Uptake and kinetic data for nutrient ions and chemically related nonnutrient analogs suggest that metabolic processes associated with root absorption of nutrients regulate both the affinity and rate of absorption of specific nonnutrient ions. Detailed kinetic studies of Ni, Cd, and Tl uptake by intact plants demonstrate multiphasic root absorption processes over a broad concentration range, and the use of transport mechanisms in place for the nutrient ions Cu, Zn, and K. Advantages and limitations of higher plants as indicators of increased levels of metal pollution are discussed in terms of these soil and plant phenomena. PMID:367766

Control of plant stem cell function by conserved interacting transcriptional regulators

PubMed Central

Zhou, Yun; Liu, Xing; Engstrom, Eric M.; Nimchuk, Zachary L.; Pruneda-Paz, Jose L.; Tarr, Paul T.; Yan, An; Kay, Steve A.; Meyerowitz, Elliot M.

2014-01-01

SUMMARY Plant stem cells in the shoot apical meristem (SAM) and root apical meristem (RAM) provide for postembryonic development of above-ground tissues and roots, respectively, while secondary vascular stem cells sustain vascular development1–4. WUSCHEL (WUS), a homeodomain transcription factor expressed in the rib meristem of the SAM, is a key regulatory factor controlling stem cell populations in the Arabidopsis SAM5–6 and is thought to establish the shoot stem cell niche via a feedback circuit with the CLAVATA3 (CLV3) peptide signaling pathway7. WUSCHEL-RELATED HOMEOBOX5 (WOX5), specifically expressed in root quiescent center (QC), defines QC identity and functions interchangeably with WUS in control of shoot and root stem cell niches8. WOX4, expressed in Arabidopsis procambial cells, defines the vascular stem cell niche9–11. WUS/WOX family proteins are evolutionarily and functionally conserved throughout the plant kingdom12 and emerge as key actors in the specification and maintenance of stem cells within all meristems13. However, the nature of the genetic regime in stem cell niches that centers on WOX gene function has been elusive, and molecular links underlying conserved WUS/WOX function in stem cell niches remain unknown. Here we demonstrate that the Arabidopsis HAIRY MERISTEM (HAM)family transcription regulators act as conserved interacting co-factors with WUS/WOX proteins. HAM and WUS share common targets in vivo and their physical interaction is important in driving downstream transcriptional programs and in promoting shoot stem cell proliferation. Differences in the overlapping expression patterns of WOX and HAM family members underlie the formation of diverse stem cell niche locations, and the HAM family is essential for all of these stem cell niches. These findings establish a new framework for the control of stem cell production during plant development. PMID:25363783

Transcription Factors Involved in Plant Resistance to Pathogens.

PubMed

Amorim, Lidiane L B; da Fonseca Dos Santos, Romulo; Neto, Joao Pacífico Bezerra; Guida-Santos, Mauro; Crovella, Sergio; Benko-Iseppon, Ana Maria

2017-01-01

Phytopathogenic microorganisms have a significant influence on survival and productivity of several crop plants. Transcription factors (TFs) are important players in the response to biotic stresses, as insect attack and pathogen infection. In face of such adversities many TFs families have been previously reported as differentially expressed in plants as a reaction to bacterial, fungal and viral infection. This review highlights recent progresses in understanding the structure, function, signal regulation and interaction of transcription factors with other proteins in response to pathogens. Hence, we focus on three families of transcription factors: ERF, bZIP and WRKY, due to their abundance, importance and the availability of functionally well-characterized members in response to pathogen attack. Their roles and the possibilities related to the use of this knowledge for engineering pathogen resistance in crop plants are also discussed. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Transcription Factor-Mediated Control of Anthocyanin Biosynthesis in Vegetative Tissues1[OPEN

PubMed Central

Outchkourov, Nikolay S.; Schrama, Xandra; Blilou, Ikram; Jongedijk, Esmer; Simon, Carmen Diez; Bosch, Dirk; Hall, Robert D.

2018-01-01

Plants accumulate secondary metabolites to adapt to environmental conditions. These compounds, here exemplified by the purple-colored anthocyanins, are accumulated upon high temperatures, UV-light, drought, and nutrient deficiencies, and may contribute to tolerance to these stresses. Producing compounds is often part of a more broad response of the plant to changes in the environment. Here we investigate how a transcription-factor-mediated program for controlling anthocyanin biosynthesis also has effects on formation of specialized cell structures and changes in the plant root architecture. A systems biology approach was developed in tomato (Solanum lycopersicum) for coordinated induction of biosynthesis of anthocyanins, in a tissue- and development-independent manner. A transcription factor couple from Antirrhinum that is known to control anthocyanin biosynthesis was introduced in tomato under control of a dexamethasone-inducible promoter. By application of dexamethasone, anthocyanin formation was induced within 24 h in vegetative tissues and in undifferentiated cells. Profiles of metabolites and gene expression were analyzed in several tomato tissues. Changes in concentration of anthocyanins and other phenolic compounds were observed in all tested tissues, accompanied by induction of the biosynthetic pathways leading from Glc to anthocyanins. A number of pathways that are not known to be involved in anthocyanin biosynthesis were observed to be regulated. Anthocyanin-producing plants displayed profound physiological and architectural changes, depending on the tissue, including root branching, root epithelial cell morphology, seed germination, and leaf conductance. The inducible anthocyanin-production system reveals a range of phenomena that accompanies anthocyanin biosynthesis in tomato, including adaptions of the plants architecture and physiology. PMID:29192027

Hormone-controlled UV-B responses in plants.

PubMed

Vanhaelewyn, Lucas; Prinsen, Els; Van Der Straeten, Dominique; Vandenbussche, Filip

2016-08-01

Ultraviolet B (UV-B) light is a portion of solar radiation that has significant effects on the development and metabolism of plants. Effects of UV-B on plants can be classified into photomorphogenic effects and stress effects. These effects largely rely on the control of, and interactions with, hormonal pathways. The fairly recent discovery of the UV-B-specific photoreceptor UV RESISTANCE LOCUS 8 (UVR8) allowed evaluation of the role of downstream hormones, leading to the identification of connections with auxin and gibberellin. Moreover, a substantial overlap between UVR8 and phytochrome responses has been shown, suggesting that part of the responses caused by UVR8 are under PHYTOCHROME INTERACTING FACTOR control. UV-B effects can also be independent of UVR8, and affect different hormonal pathways. UV-B affects hormonal pathways in various ways: photochemically, affecting biosynthesis, transport, and/or signaling. This review concludes that the effects of UV-B on hormonal regulation can be roughly divided in two: inhibition of growth-promoting hormones; and the enhancement of environmental stress-induced defense hormones. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

SOWFA Super-Controller: A High-Fidelity Tool for Evaluating Wind Plant Control Approaches

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fleming, P.; Gebraad, P.; van Wingerden, J. W.

2013-01-01

This paper presents a new tool for testing wind plant controllers in the Simulator for Offshore Wind Farm Applications (SOWFA). SOWFA is a high-fidelity simulator for the interaction between wind turbine dynamics and the fluid flow in a wind plant. The new super-controller testing environment in SOWFA allows for the implementation of the majority of the wind plant control strategies proposed in the literature.

Optimization of EB plant by constraint control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hummel, H.K.; de Wit, G.B.C.; Maarleveld, A.

1991-03-01

Optimum plant operation can often be achieved by means of constraint control instead of model- based on-line optimization. This is because optimum operation is seldom at the top of the hill but usually at the intersection of constraints. This article describes the development of a constraint control system for a plant producing ethylbenzene (EB) by the Mobil/Badger Ethylbenzene Process. Plant optimization can be defined as the maximization of a profit function describing the economics of the plant. This function contains terms with product values, feedstock prices and operational costs. Maximization of the profit function can be obtained by varying relevantmore » degrees of freedom in the plant, such as a column operating pressure or a reactor temperature. These degrees of freedom can be varied within the available operating margins of the plant.« less

Effects of herbaceous and woody plant control on longleaf pine growth and understory plant cover

Treesearch

James D. Haywood

2013-01-01

To determine if either herbaceous or woody plants are more competitive with longleaf pine (Pinus palustris Mill.) trees, four vegetation management treatments— check, herbaceous plant control (HPC), woody plant control (WPC), and HPC+WPC—were applied in newly established longleaf pine plantings in a randomized complete block design in two studies....

A Framework for Engineering Stress Resilient Plants Using Genetic Feedback Control and Regulatory Network Rewiring.

PubMed

Foo, Mathias; Gherman, Iulia; Zhang, Peijun; Bates, Declan G; Denby, Katherine J

2018-05-23

Crop disease leads to significant waste worldwide, both pre- and postharvest, with subsequent economic and sustainability consequences. Disease outcome is determined both by the plants' response to the pathogen and by the ability of the pathogen to suppress defense responses and manipulate the plant to enhance colonization. The defense response of a plant is characterized by significant transcriptional reprogramming mediated by underlying gene regulatory networks, and components of these networks are often targeted by attacking pathogens. Here, using gene expression data from Botrytis cinerea-infected Arabidopsis plants, we develop a systematic approach for mitigating the effects of pathogen-induced network perturbations, using the tools of synthetic biology. We employ network inference and system identification techniques to build an accurate model of an Arabidopsis defense subnetwork that contains key genes determining susceptibility of the plant to the pathogen attack. Once validated against time-series data, we use this model to design and test perturbation mitigation strategies based on the use of genetic feedback control. We show how a synthetic feedback controller can be designed to attenuate the effect of external perturbations on the transcription factor CHE in our subnetwork. We investigate and compare two approaches for implementing such a controller biologically-direct implementation of the genetic feedback controller, and rewiring the regulatory regions of multiple genes-to achieve the network motif required to implement the controller. Our results highlight the potential of combining feedback control theory with synthetic biology for engineering plants with enhanced resilience to environmental stress.

Comparative analysis of cells and proteins of pumpkin plants for the control of fruit size.

PubMed

Nakata, Yumiko; Taniguchi, Go; Takazaki, Shinya; Oda-Ueda, Naoko; Miyahara, Kohji; Ohshima, Yasumi

2012-09-01

Common pumpkin plants (Cucurbita maxima) produce fruits of 1-2 kg size on the average, while special varieties of the same species called Atlantic Giant are known to produce a huge fruit up to several hundred kilograms. As an approach to determine the factors controlling the fruit size in C. maxima, we cultivated both AG and control common plants, and found that both the cell number and cell sizes were increased in a large fruit while DNA content of the cell did not change significantly. We also compared protein patterns in the leaves, stems, ripe and young fruits by two-dimensional (2D) gel electrophoresis, and identified those differentially expressed between them with mass spectroscopy. Based on these results, we suggest that factors in photosynthesis such as ribulose-bisphosphate carboxylase, glycolysis pathway enzymes, heat-shock proteins and ATP synthase play positive or negative roles in the growth of a pumpkin fruit. These results provide a step toward the development of plant biotechnology to control fruit size in the future. Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

The sunflower transcription factor HaWRKY76 confers drought and flood tolerance to Arabidopsis thaliana plants without yield penalty.

PubMed

Raineri, Jesica; Ribichich, Karina F; Chan, Raquel L

2015-12-01

Arabidopsis transgenic plants expressing the sunflower transcription factor HaWRKY76 exhibit increased yield and tolerance to drought and flood stresses. The genetic construct containing HaWRKY76 is proposed as a potential biotechnological tool to improve crops. Water deficit and water excess are abiotic stress factors that seriously affect crops worldwide. To increase the tolerance to such stresses without causing yield penalty constitutes a major goal for biotechnologists. In this survey, we report that HaWRKY76, a divergent sunflower WRKY transcription factor, is able to confer both dehydration and submergence tolerance to Arabidopsis transgenic plants without yield penalty. The expression pattern of HaWRKY76 was analyzed in plants grown in standard conditions and under different watering regimes indicating a regulation by water availability. The corresponding cDNA was isolated and cloned under the control of a constitutive promoter and Arabidopsis plants were transformed with this construct. These transgenic plants presented higher biomass, seed production and sucrose content than controls in standard growth conditions. Moreover, they exhibited tolerance to mild drought or flood (complete submergence/waterlogging) stresses as well as the same or increased yield, depending on the stress severity and plant developmental stage, compared with controls. Drought tolerance occurred via an ABA-independent mechanism and induction of stomatal closure. Submergence tolerance can be explained by the carbohydrate (sucrose and starch) preservation achieved through the repression of fermentation pathways. Higher cell membrane stability and chlorenchyma maintenance could be the nexus between tolerance responses in front of both stresses. Altogether, the obtained results indicated that HaWRKY76 can be a potential biotechnological tool to improve crops yield as well as drought and flood tolerances.

Phenological modifications in plants by various edaphic factors.

PubMed

Wielgolaski, F E

2001-11-01

Various mechanical, chemical and physical soil analyses were carried out, in addition to weather observations, for 3 years at several sites along an oceanic-continental gradient in a fjord district in western Norway. All the environmental factors observed were correlated with the spring and a few late-season phenophases of many native and cultivated woody plants and some herbs by simple, linear correlations and by stepwise multiple and partial analyses. Different techniques were used to try and eliminate many intercorrelations between various environmental factors. As expected, air temperature measurements in nearly all analyses from these temperate region districts gave the most significant correlations with the phenology of the plants, the temperature during the night generally being the most important in mainly vegetative periods, e.g. to leaf bud break in spring, and the temperature during the day affecting the more generative phases, such as the period between leaf bud break and flowering. The other environmental factors, however, showed strong variation in correlation significance among the various species studied and also with different phenophases of the same species. Various hypotheses are put forward to explain such variation. Air humidity (including precipitation) and/or soil moisture (including intercorrelated parameters, e.g. soil grain size and bulk density) were relatively often found to be of importance. In the stepwise multiple analyses for leaf bud break of the birch (Betula pubescens), for instance, the amount of precipitation was the second factor to enter the analyses by a positive correlation with the developmental rate, after the most important factor, the night temperature. Positive correlations with a high clay content and bulk density in the soil indicated that high soil moisture is also favourable for early bud break in the birch. Other phenophases that seemed to be favoured by a good water supply were leaf bud break of the bird cherry

Cyt toxin expression reveals an inverse regulation of insect and plant virulence factors of Dickeya dadantii.

PubMed

Costechareyre, Denis; Dridi, Bedis; Rahbé, Yvan; Condemine, Guy

2010-12-01

The plant pathogenic bacteria Dickeya dadantii is also a pathogen of the pea aphid Acyrthosiphon pisum. The genome of the bacteria contains four cyt genes, encoding homologues of Bacillus thuringiensis Cyt toxins, which are involved in its pathogenicity to insects. We show here that these genes are transcribed as an operon, and we determined the conditions necessary for their expression. Their expression is induced at high temperature and at an osmolarity equivalent to that found in the plant phloem sap. The regulators of cyt genes have also been identified: their expression is repressed by H-NS and VfmE and activated by PecS. These genes are already known to regulate plant virulence factors, but in an opposite way. When tested in a virulence assay by ingestion, the pecS mutant was almost non-pathogenic while hns and vfmE mutants behaved in the same way as the wild-type strain. Mutants of other regulators of plant virulence, GacA, OmpR and PhoP, that do not control Cyt toxin production, also showed reduced pathogenicity. In an assay by injection of bacteria, the gacA strain was less pathogenic but, surprisingly, the pecS mutant was slightly more virulent. These results show that Cyt toxins are not the only virulence factors required to kill aphids, and that these factors act at different stages of the infection. Moreover, their production is controlled by general virulence regulators known for their role in plant virulence. This integration could indicate that virulence towards insects is a normal mode of life for D. dadantii. © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.

Steam plant startup and control in system restoration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mello, F.P. de; Westcott, J.C.

1994-02-01

The IEEE Working Group on Power System Restoration developed a panel session for the Summer Power Meeting on July 14, 1992 on Special Considerations in Power System Restoration. One of the contributions to this session is presented in this paper dealing with aspects of steam plant startup and control in scenarios of system restoration. The topics addressed include the complexity of a steam plant, the contrast between normal plant startups and shutdowns and those following major system blackouts including the effects of plant design, automatic controls, bypass valving and operator training.

A review of major factors influencing plant responses to recreation impacts

NASA Astrophysics Data System (ADS)

Kuss, Fred R.

1986-09-01

This article reviews some of the more important factors found to influence the susceptibility of plants to trampling impacts associated with recreational use of natural areas. A three-way interaction mediates plant responses to impacts: plant x environment x stress level(s). Plant responses vary in part according to the genetic constitution of the plant, life and growth form, the adaptive flexibility of the plant, and anatomical differences inherent to growth habit and morphology. Other factors that influence plant sensitivities to impacts are the habitat environments in which plants grow, since a number of conditions such as moisture excesses or deficiencies, nitrogen or oxygen starvation, late frosts, etc., cause physiological injury and may increase plant sensitivity to impacts. Among the environmental factors that may increase or lessen plant sensitivities to impacts are soil moisture levels, canopy density, elevation, aspect, microclimate, soil drainage, texture, fertility and productivity. Seasonal influences also bear consideration since environmental changes and phonological and physiological events are mediated by time of year. Stresses are caused by both direct and indirect forms of impact and vary according to season of use, frequency and amount of use, and the type of activity. These interactions are further complicated by evidence that inter- and intraspecific competition, antagonism, and commensalism may influence differences in the sensitivity of plant communities to impacts.

Heavy Metal Concentrations in Soils and Factors Affecting Metal Uptake by Plants in the Vicinity of a Korean Cu-W Mine.

PubMed

Jung, Myung Chae

2008-04-04

Heavy metal concentrations were measured in soils and plants in and around a copper-tungsten mine in southeast Korea to investigate the influence of past base metal mining on the surface environment. The results of chemical analysis indicate that the heavy metals in soils decreased with distance from the source, controlled mainly by water movement and topography. The metal concentrations measured in plant species generally decreased in the order; spring onions > soybean leaves > perilla leaves » red pepper > corn grains » jujube grains, although this pattern varied moderately between different elements. The results agree with other reports that metal concentrations in leaves are usually much higher than those in grain. Factors influencing the bioavailability of metals and their occurrences in crops were found as soil pH, cation exchange capacity, organic matter content, soil texture, and interaction among the target elements. It is concluded that total metal concentrations in soils are the main controls on their contents in plants. Soil pH was also an important factor. A stepwise linear multiple regression analysis was also conducted to identify the dominant factors influencing metal uptake by plants. Metal concentrations in plants were also estimated by computer-aided statistical methods.

Plant productivity in controlled environments

NASA Technical Reports Server (NTRS)

Salisbury, F. B.; Bugbee, B.

1988-01-01

To assess the cost and area/volume requirements of a farm in a space station or Lunar or Martian base, a few laboratories in the United States, the Soviet Union, France, and Japan are studying optimum controlled environments for the production of selected crops. Temperature, light, photoperiod, CO2, humidity, the root-zone environment, and cultivars are the primary factors being manipulated to increase yields and harvest index. Our best wheat yields on a time basis (24 g m-2 day-1 of edible biomass) are five times good field yields and twice the world record. Similar yields have been obtained in other laboratories with potatoes and lettuce; soybeans are also promising. These figures suggest that approximately 30 m2 under continuous production could support an astronaut with sufficient protein and about 2800 kcal day-1. Scientists under Iosif Gitelzon in Krasnoyarsk, Siberia, have lived in a closed system for up to 5 months, producing 80% of their own food. Thirty square meters for crops were allotted to each of the two men taking part in the experiment. A functional controlled-environment life-support system (CELSS) will require the refined application of several disciplines: controlled-environment agriculture, food preparation, waste disposal, and control-systems technology, to list only the broadest categories. It has seemed intuitively evident that ways could be found to prepare food, regenerate plant nutrients from wastes, and even control and integrate several subsystems of a CELSS. But could sufficient food be produced in the limited areas and with the limited energy that might be available? Clearly, detailed studies of food production were necessary.

Plant productivity in controlled environments.

PubMed

Salisbury, F B; Bugbee, B

1988-04-01

To assess the cost and area/volume requirements of a farm in a space station or Lunar or Martian base, a few laboratories in the United States, the Soviet Union, France, and Japan are studying optimum controlled environments for the production of selected crops. Temperature, light, photoperiod, CO2, humidity, the root-zone environment, and cultivars are the primary factors being manipulated to increase yields and harvest index. Our best wheat yields on a time basis (24 g m-2 day-1 of edible biomass) are five times good field yields and twice the world record. Similar yields have been obtained in other laboratories with potatoes and lettuce; soybeans are also promising. These figures suggest that approximately 30 m2 under continuous production could support an astronaut with sufficient protein and about 2800 kcal day-1. Scientists under Iosif Gitelzon in Krasnoyarsk, Siberia, have lived in a closed system for up to 5 months, producing 80% of their own food. Thirty square meters for crops were allotted to each of the two men taking part in the experiment. A functional controlled-environment life-support system (CELSS) will require the refined application of several disciplines: controlled-environment agriculture, food preparation, waste disposal, and control-systems technology, to list only the broadest categories. It has seemed intuitively evident that ways could be found to prepare food, regenerate plant nutrients from wastes, and even control and integrate several subsystems of a CELSS. But could sufficient food be produced in the limited areas and with the limited energy that might be available? Clearly, detailed studies of food production were necessary.

New Process Controls for the Hera Cryogenic Plant

NASA Astrophysics Data System (ADS)

Böckmann, T.; Clausen, M.; Gerke, Chr.; Prüß, K.; Schoeneburg, B.; Urbschat, P.

2010-04-01

The cryogenic plant built for the HERA accelerator at DESY in Hamburg (Germany) is now in operation for more than two decades. The commercial process control system for the cryogenic plant is in operation for the same time period. Ever since the operator stations, the control network and the CPU boards in the process controllers went through several upgrade stages. Only the centralized Input/Output system was kept unchanged. Many components have been running beyond the expected lifetime. The control system for one at the three parts of the cryogenic plant has been replaced recently by a distributed I/O system. The I/O nodes are connected to several Profibus-DP field busses. Profibus provides the infrastructure to attach intelligent sensors and actuators directly to the process controllers which run the open source process control software EPICS. This paper describes the modification process on all levels from cabling through I/O configuration, the process control software up to the operator displays.

Control of Fish and Aquatic Plants.

ERIC Educational Resources Information Center

Hesser, R. B.; And Others

This agriculture extension service publication from Pennsylvania State University is a handbook for the water body manager. The bulk of the contents deals with aquatic plant control. The different types of aquatic plants, their reproduction and growth, and their role in the ecology of the water body are introduced in this main section. Also, the…

Factors controlling dimethylsulfide emission from salt marshes

NASA Technical Reports Server (NTRS)

Dacey, John W. H.; Wakeham, S. G.; Howes, B. L.

1985-01-01

The factors that control the emission of methylated gases from salt marshes are being studied. Research focusses on dimethylsulfide (DMS) formation and the mechanism of DMS and CH4 emission to the atmosphere. The approach is to consider the plants as valves regulating the emission of methylated gases to the atmosphere with the goal of developing appropriate methods for emission measurement. In the case of CH4, the sediment is the source and transport to the atmosphere occurs primarily through the internal gas spaces in the plants. The source of DMS appears to be dimethyl sulfoniopropionate (DMSP) which may play a role in osmoregulation in plant tissues. Concentrations of DMSP in leaves are typically several-fold higher than in roots and rhizomes. Even so, the large below ground biomass of this plant means that 2/3 of the DMSP in the ecosystem is below ground on the aerial basis. Upon introduction to sediment water, DMSP rapidly decomposes to DMS and acrylic acid. The solubility of a gas (its equilibrium vapor pressure) is a fundamental aspect of gas exchange kinetics. The first comprehensive study was conducted of DMS solubility in freshwater and seawater. Data suggest that the Setchenow relation holds for H at intermediate salinities collected. These data support the concept that the concentration of DMS in the atmosphere is far from equilibrium with seawater.

Antimicrobial peptides and plant disease control.

PubMed

Montesinos, Emilio

2007-05-01

Several diseases caused by viruses, bacteria and fungi affect plant crops, resulting in losses and decreasing the quality and safety of agricultural products. Plant disease control relies mainly on chemical pesticides that are currently subject to strong restrictions and regulatory requirements. Antimicrobial peptides are interesting compounds in plant health because there is a need for new products in plant protection that fit into the new regulations. Living organisms secrete a wide range of antimicrobial peptides produced through ribosomal (defensins and small bacteriocins) or non-ribosomal synthesis (peptaibols, cyclopeptides and pseudopeptides). Several antimicrobial peptides are the basis for the design of new synthetic analogues, have been expressed in transgenic plants to confer disease protection or are secreted by microorganisms that are active ingredients of commercial biopesticides.

Genetics-based control of a mimo boiler-turbine plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dimeo, R.M.; Lee, K.Y.

1994-12-31

A genetic algorithm is used to develop an optimal controller for a non-linear, multi-input/multi-output boiler-turbine plant. The algorithm is used to train a control system for the plant over a wide operating range in an effort to obtain better performance. The results of the genetic algorithm`s controller designed from the linearized plant model at a nominal operating point. Because the genetic algorithm is well-suited to solving traditionally difficult optimization problems it is found that the algorithm is capable of developing the controller based on input/output information only. This controller achieves a performance comparable to the standard linear quadratic regulator.

Plant-Produced Human Recombinant Erythropoietic Growth Factors Support Erythroid Differentiation In Vitro

PubMed Central

Musiychuk, Konstantin; Sivalenka, Rajarajeswari; Jaje, Jennifer; Bi, Hong; Flores, Rosemary; Shaw, Brenden; Jones, R. Mark; Golovina, Tatiana; Schnipper, Jacob; Khandker, Luipa; Sun, Ruiqiang; Li, Chang; Kang, Lin; Voskinarian-Berse, Vanessa; Zhang, Xiaokui; Streatfield, Stephen; Hambor, John; Abbot, Stewart

2013-01-01

Clinically available red blood cells (RBCs) for transfusions are at high demand, but in vitro generation of RBCs from hematopoietic stem cells requires significant quantities of growth factors. Here, we describe the production of four human growth factors: erythropoietin (EPO), stem cell factor (SCF), interleukin 3 (IL-3), and insulin-like growth factor-1 (IGF-1), either as non-fused proteins or as fusions with a carrier molecule (lichenase), in plants, using a Tobacco mosaic virus vector-based transient expression system. All growth factors were purified and their identity was confirmed by western blotting and peptide mapping. The potency of these plant-produced cytokines was assessed using TF1 cell (responsive to EPO, IL-3 and SCF) or MCF-7 cell (responsive to IGF-1) proliferation assays. The biological activity estimated here for the cytokines produced in plants was slightly lower or within the range cited in commercial sources and published literature. By comparing EC50 values of plant-produced cytokines with standards, we have demonstrated that all four plant-produced growth factors stimulated the expansion of umbilical cord blood-derived CD34+ cells and their differentiation toward erythropoietic precursors with the same potency as commercially available growth factors. To the best of our knowledge, this is the first report on the generation of all key bioactive cytokines required for the erythroid development in a cost-effective manner using a plant-based expression system. PMID:23517237

Structured plant metabolomics for the simultaneous exploration of multiple factors.

PubMed

Vasilev, Nikolay; Boccard, Julien; Lang, Gerhard; Grömping, Ulrike; Fischer, Rainer; Goepfert, Simon; Rudaz, Serge; Schillberg, Stefan

2016-11-17

Multiple factors act simultaneously on plants to establish complex interaction networks involving nutrients, elicitors and metabolites. Metabolomics offers a better understanding of complex biological systems, but evaluating the simultaneous impact of different parameters on metabolic pathways that have many components is a challenging task. We therefore developed a novel approach that combines experimental design, untargeted metabolic profiling based on multiple chromatography systems and ionization modes, and multiblock data analysis, facilitating the systematic analysis of metabolic changes in plants caused by different factors acting at the same time. Using this method, target geraniol compounds produced in transgenic tobacco cell cultures were grouped into clusters based on their response to different factors. We hypothesized that our novel approach may provide more robust data for process optimization in plant cell cultures producing any target secondary metabolite, based on the simultaneous exploration of multiple factors rather than varying one factor each time. The suitability of our approach was verified by confirming several previously reported examples of elicitor-metabolite crosstalk. However, unravelling all factor-metabolite networks remains challenging because it requires the identification of all biochemically significant metabolites in the metabolomics dataset.

Is the efficacy of biological control against plant diseases likely to be more durable than that of chemical pesticides?

PubMed Central

Bardin, Marc; Ajouz, Sakhr; Comby, Morgane; Lopez-Ferber, Miguel; Graillot, Benoît; Siegwart, Myriam; Nicot, Philippe C.

2015-01-01

The durability of a control method for plant protection is defined as the persistence of its efficacy in space and time. It depends on (i) the selection pressure exerted by it on populations of plant pathogens and (ii) on the capacity of these pathogens to adapt to the control method. Erosion of effectiveness of conventional plant protection methods has been widely studied in the past. For example, apparition of resistance to chemical pesticides in plant pathogens or pests has been extensively documented. The durability of biological control has often been assumed to be higher than that of chemical control. Results concerning pest management in agricultural systems have shown that this assumption may not always be justified. Resistance of various pests to one or several toxins of Bacillus thuringiensis and apparition of resistance of the codling moth Cydia pomonella to the C. pomonella granulovirus have, for example, been described. In contrast with the situation for pests, the durability of biological control of plant diseases has hardly been studied and no scientific reports proving the loss of efficiency of biological control agents against plant pathogens in practice has been published so far. Knowledge concerning the possible erosion of effectiveness of biological control is essential to ensure a durable efficacy of biological control agents on target plant pathogens. This knowledge will result in identifying risk factors that can foster the selection of strains of plant pathogens resistant to biological control agents. It will also result in identifying types of biological control agents with lower risk of efficacy loss, i.e., modes of action of biological control agents that does not favor the selection of resistant isolates in natural populations of plant pathogens. An analysis of the scientific literature was then conducted to assess the potential for plant pathogens to become resistant to biological control agents. PMID:26284088

Control of Floral Meristem Determinacy in Petunia by MADS-Box Transcription Factors1[W

PubMed Central

Ferrario, Silvia; Shchennikova, Anna V.; Franken, John; Immink, Richard G.H.; Angenent, Gerco C.

2006-01-01

The shoot apical meristem (SAM), a small group of undifferentiated dividing cells, is responsible for the continuous growth of plants. Several genes have been identified that control the development and maintenance of the SAM. Among these, WUSCHEL (WUS) from Arabidopsis (Arabidopsis thaliana) is thought to be required for maintenance of a stem cell pool in the SAM. The MADS-box gene AGAMOUS, in combination with an unknown factor, has been proposed as a possible negative regulator of WUS, leading to the termination of meristematic activity within the floral meristem. Transgenic petunia (Petunia hybrida) plants were produced in which the E-type and D-type MADS-box genes FLORAL BINDING PROTEIN2 (FBP2) and FBP11, respectively, are simultaneously overexpressed. These plants show an early arrest in development at the cotyledon stage. Molecular analysis of these transgenic plants revealed a possible combined action of FBP2 and FBP11 in repressing the petunia WUS homolog, TERMINATOR. Furthermore, the ectopic up-regulation of the C-type and D-type homeotic genes FBP6 and FBP7, respectively, suggests that they may also participate in a complex, which causes the determinacy in transgenic plants. These data support the model that a transcription factor complex consisting of C-, D-, and E-type MADS-box proteins controls the stem cell population in the floral meristem. PMID:16428599

Microprocessor-based control systems application in nuclear power plant critical systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shah, M.R.; Nowak, J.B.

Microprocessor-based control systems have been used in fossil power plants and are receiving greater acceptance for application in nuclear plants. This technology is not new but it does require unique considerations when applied to nuclear power plants. Sargent and Lundy (S and L) has used a microprocessor-based component logic control system (interposing Logic System) for safety- and non-safety-related components in nuclear power plants under construction overseas. Currently, S and L is in the design stage to replace an existing analog control system with a microprocessor-based control system in the U.S. The trend in the industry is to replace systems inmore » existing plants or design new power plants with microprocessor-based control systems.« less

Parasitic Cuscuta factor(s) and the detection by tomato initiates plant defense.

PubMed

Fürst, Ursula; Hegenauer, Volker; Kaiser, Bettina; Körner, Max; Welz, Max; Albert, Markus

2016-01-01

Dodders ( Cuscuta spp.) are holoparasitic plants that enwind stems of host plants and penetrate those by haustoria to connect to the vascular bundles. Having a broad host plant spectrum, Cuscuta spp infect nearly all dicot plants - only cultivated tomato as one exception is mounting an active defense specifically against C. reflexa . In a recent work we identified a pattern recognition receptor of tomato, "Cuscuta Receptor 1" (CuRe1), which is critical to detect a "Cuscuta factor" (CuF) and initiate defense responses such as the production of ethylene or the generation of reactive oxygen species. CuRe1 also contributes to the tomato resistance against C. reflexa . Here we point to the fact that CuRe1 is not the only relevant component for full tomato resistance but it requires additional defense mechanisms, or receptors, respectively, to totally fend off the parasite.

Model-free adaptive control of advanced power plants

DOEpatents

Cheng, George Shu-Xing; Mulkey, Steven L.; Wang, Qiang

2015-08-18

A novel 3-Input-3-Output (3.times.3) Model-Free Adaptive (MFA) controller with a set of artificial neural networks as part of the controller is introduced. A 3.times.3 MFA control system using the inventive 3.times.3 MFA controller is described to control key process variables including Power, Steam Throttle Pressure, and Steam Temperature of boiler-turbine-generator (BTG) units in conventional and advanced power plants. Those advanced power plants may comprise Once-Through Supercritical (OTSC) Boilers, Circulating Fluidized-Bed (CFB) Boilers, and Once-Through Supercritical Circulating Fluidized-Bed (OTSC CFB) Boilers.

Abscisic acid controlled sex before transpiration in vascular plants.

PubMed

McAdam, Scott A M; Brodribb, Timothy J; Banks, Jo Ann; Hedrich, Rainer; Atallah, Nadia M; Cai, Chao; Geringer, Michael A; Lind, Christof; Nichols, David S; Stachowski, Kye; Geiger, Dietmar; Sussmilch, Frances C

2016-10-26

Sexual reproduction in animals and plants shares common elements, including sperm and egg production, but unlike animals, little is known about the regulatory pathways that determine the sex of plants. Here we use mutants and gene silencing in a fern species to identify a core regulatory mechanism in plant sexual differentiation. A key player in fern sex differentiation is the phytohormone abscisic acid (ABA), which regulates the sex ratio of male to hermaphrodite tissues during the reproductive cycle. Our analysis shows that in the fern Ceratopteris richardii, a gene homologous to core ABA transduction genes in flowering plants [SNF1-related kinase2s (SnRK2s)] is primarily responsible for the hormonal control of sex determination. Furthermore, we provide evidence that this ABA-SnRK2 signaling pathway has transitioned from determining the sex of ferns to controlling seed dormancy in the earliest seed plants before being co-opted to control transpiration and CO 2 exchange in derived seed plants. By tracing the evolutionary history of this ABA signaling pathway from plant reproduction through to its role in the global regulation of plant-atmosphere gas exchange during the last 450 million years, we highlight the extraordinary effect of the ABA-SnRK2 signaling pathway in plant evolution and vegetation function.

Sociocultural and ecological factors influencing management of edible and non-edible plants: the case of Ixcatlán, Mexico.

PubMed

Rangel-Landa, Selene; Casas, Alejandro; García-Frapolli, Eduardo; Lira, Rafael

2017-10-30

Identifying factors influencing plant management allows understanding how processes of domestication operate. Uncertain availability of resources is a main motivation for managing edible plants, but little is known about management motives of non-edible resources like medicinal and ceremonial plants. We hypothesized that uncertain availability of resources would be a general factor motivating their management, but other motives could operate simultaneously. Uncertainty and risk might be less important motives in medicinal than in edible plants, while for ceremonial plants, symbolic and spiritual values would be more relevant. We inventoried edible, medicinal, and ceremonial plants in Ixcatlán, Oaxaca, Mexico, and conducted in-depth studies with 20 native and naturalized species per use type; we documented their cultural importance and abundance by interviewing 25 households and sampling vegetation in 33 sites. Consumption amounts and preferences were studied through surveys and free listings with 38 interviewees. Management intensity and risk indexes were calculated through PCA and their relation analyzed through regression analyses. Canonical methods allowed identifying the main sociocultural and ecological factors influencing management of plants per use type. Nearly 64, 63, and 55% of all ceremonial, edible, and medicinal wild plants recorded, respectively, are managed in order to maintain or increase their availability, embellishing environments, and because of ethical reasons and curiosity. Management intensity was higher in edible plants under human selection and associated with risk. Management of ceremonial and medicinal plants was not associated with indexes of risk or uncertainty in their availability. Other sociocultural and ecological factors influence management intensity, the most important being reciprocal relations and abundance perception. Plant management through practices and collectively regulated strategies is strongly related to control of

High-autonomy control of space resource processing plants

NASA Technical Reports Server (NTRS)

Schooley, Larry C.; Zeigler, Bernard P.; Cellier, Francois E.; Wang, Fei-Yue

1993-01-01

A highly autonomous intelligent command/control architecture has been developed for planetary surface base industrial process plants and Space Station Freedom experimental facilities. The architecture makes use of a high-level task-oriented mode with supervisory control from one or several remote sites, and integrates advanced network communications concepts and state-of-the-art man/machine interfaces with the most advanced autonomous intelligent control. Attention is given to the full-dynamics model of a Martian oxygen-production plant, event-based/fuzzy-logic process control, and fault management practices.

Factors influencing workers to follow food safety management systems in meat plants in Ontario, Canada.

PubMed

Ball, Brita; Wilcock, Anne; Aung, May

2009-06-01

Small and medium sized food businesses have been slow to adopt food safety management systems (FSMSs) such as good manufacturing practices and Hazard Analysis Critical Control Point (HACCP). This study identifies factors influencing workers in their implementation of food safety practices in small and medium meat processing establishments in Ontario, Canada. A qualitative approach was used to explore in-plant factors that influence the implementation of FSMSs. Thirteen in-depth interviews in five meat plants and two focus group interviews were conducted. These generated 219 pages of verbatim transcripts which were analysed using NVivo 7 software. Main themes identified in the data related to production systems, organisational characteristics and employee characteristics. A socio-psychological model based on the theory of planned behaviour is proposed to describe how these themes and underlying sub-themes relate to FSMS implementation. Addressing the various factors that influence production workers is expected to enhance FSMS implementation and increase food safety.

Indicator system for advanced nuclear plant control complex

DOEpatents

Scarola, Kenneth; Jamison, David S.; Manazir, Richard M.; Rescorl, Robert L.; Harmon, Daryl L.

1993-01-01

An advanced control room complex for a nuclear power plant, including a discrete indicator and alarm system (72) which is nuclear qualified for rapid response to changes in plant parameters and a component control system (64) which together provide a discrete monitoring and control capability at a panel (14-22, 26, 28) in the control room (10). A separate data processing system (70), which need not be nuclear qualified, provides integrated and overview information to the control room and to each panel, through CRTs (84) and a large, overhead integrated process status overview board (24). The discrete indicator and alarm system (72) and the data processing system (70) receive inputs from common plant sensors and validate the sensor outputs to arrive at a representative value of the parameter for use by the operator during both normal and accident conditions, thereby avoiding the need for him to assimilate data from each sensor individually. The integrated process status board (24) is at the apex of an information hierarchy that extends through four levels and provides access at each panel to the full display hierarchy. The control room panels are preferably of a modular construction, permitting the definition of inputs and outputs, the man machine interface, and the plant specific algorithms, to proceed in parallel with the fabrication of the panels, the installation of the equipment and the generic testing thereof.

Indicator system for a process plant control complex

DOEpatents

Scarola, Kenneth; Jamison, David S.; Manazir, Richard M.; Rescorl, Robert L.; Harmon, Daryl L.

1993-01-01

An advanced control room complex for a nuclear power plant, including a discrete indicator and alarm system (72) which is nuclear qualified for rapid response to changes in plant parameters and a component control system (64) which together provide a discrete monitoring and control capability at a panel (14-22, 26, 28) in the control room (10). A separate data processing system (70), which need not be nuclear qualified, provides integrated and overview information to the control room and to each panel, through CRTs (84) and a large, overhead integrated process status overview board (24). The discrete indicator and alarm system (72) and the data processing system (70) receive inputs from common plant sensors and validate the sensor outputs to arrive at a representative value of the parameter for use by the operator during both normal and accident conditions, thereby avoiding the need for him to assimilate data from each sensor individually. The integrated process status board (24) is at the apex of an information hierarchy that extends through four levels and provides access at each panel to the full display hierarchy. The control room panels are preferably of a modular construction, permitting the definition of inputs and outputs, the man machine interface, and the plant specific algorithms, to proceed in parallel with the fabrication of the panels, the installation of the equipment and the generic testing thereof.

FRAMEWORK AND APPLICATION FOR MODELING CONTROL ROOM CREW PERFORMANCE AT NUCLEAR POWER PLANTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ronald L Boring; David I Gertman; Tuan Q Tran

2008-09-01

This paper summarizes an emerging project regarding the utilization of high-fidelity MIDAS simulations for visualizing and modeling control room crew performance at nuclear power plants. The key envisioned uses for MIDAS-based control room simulations are: (i) the estimation of human error associated with advanced control room equipment and configurations, (ii) the investigative determination of contributory cognitive factors for risk significant scenarios involving control room operating crews, and (iii) the certification of reduced staffing levels in advanced control rooms. It is proposed that MIDAS serves as a key component for the effective modeling of cognition, elements of situation awareness, and riskmore » associated with human performance in next generation control rooms.« less

Abscisic acid controlled sex before transpiration in vascular plants

PubMed Central

McAdam, Scott A. M.; Brodribb, Timothy J.; Hedrich, Rainer; Atallah, Nadia M.; Cai, Chao; Geringer, Michael A.; Lind, Christof; Nichols, David S.; Stachowski, Kye; Sussmilch, Frances C.

2016-01-01

Sexual reproduction in animals and plants shares common elements, including sperm and egg production, but unlike animals, little is known about the regulatory pathways that determine the sex of plants. Here we use mutants and gene silencing in a fern species to identify a core regulatory mechanism in plant sexual differentiation. A key player in fern sex differentiation is the phytohormone abscisic acid (ABA), which regulates the sex ratio of male to hermaphrodite tissues during the reproductive cycle. Our analysis shows that in the fern Ceratopteris richardii, a gene homologous to core ABA transduction genes in flowering plants [SNF1-related kinase2s (SnRK2s)] is primarily responsible for the hormonal control of sex determination. Furthermore, we provide evidence that this ABA–SnRK2 signaling pathway has transitioned from determining the sex of ferns to controlling seed dormancy in the earliest seed plants before being co-opted to control transpiration and CO2 exchange in derived seed plants. By tracing the evolutionary history of this ABA signaling pathway from plant reproduction through to its role in the global regulation of plant–atmosphere gas exchange during the last 450 million years, we highlight the extraordinary effect of the ABA–SnRK2 signaling pathway in plant evolution and vegetation function. PMID:27791082

Automatic control of solar power plants

NASA Astrophysics Data System (ADS)

Ermakov, V. S.; Dubilovich, V. M.

1982-02-01

The automatic control of the heliostat field of a 200-MW solar power plant is discussed. The advantages of the decentralized control principle with the solution of a number of individual problems in a single control center are emphasized. The basic requirements on heliostat construction are examined, and possible functional schemes for the automatic control of a heliostat field are described. It is proposed that groups of heliostats can be controlled from a single center and on the basis of a single algorithm.

Basic leucine zipper domain transcription factors: the vanguards in plant immunity.

PubMed

Noman, Ali; Liu, Zhiqin; Aqeel, Muhammad; Zainab, Madiha; Khan, Muhammad Ifnan; Hussain, Ansar; Ashraf, Muhammad Furqan; Li, Xia; Weng, Yahong; He, Shuilin

2017-12-01

Regulation of spatio-temporal expression patterns of stress tolerance associated plant genes is an essential component of the stress responses. Eukaryotes assign a large amount of their genome to transcription with multiple transcription factors (TFs). Often, these transcription factors fit into outsized gene groups which, in several cases, exclusively belong to plants. Basic leucine zipper domain (bZIP) transcription factors regulate vital processes in plants and animals. In plants, bZIPs are implicated in numerous fundamental processes like seed development, energy balance, and responses to abiotic or biotic stresses. Systematic analysis of the information obtained over the last two decades disclosed a constitutive role of bZIPs against biotic stress. bZIP TFs are vital players in plant innate immunity due to their ability to regulate genes associated with PAMP-triggered immunity, effector-triggered immunity, and hormonal signaling networks. Expression analysis of studied bZIP genes suggests that exploration and functional characterization of novel bZIP TFs in planta is helpful in improving crop resistance against pathogens and environmental stresses. Our review focuses on major advancements in bZIP TFs and plant responses against different pathogens. The integration of genomics information with the functional studies provides new insights into the regulation of plant defense mechanisms and engineering crops with improved resistance to invading pathogens. Conclusively, succinct functions of bZIPs as positive or negative regulator mediate resistance to the plant pathogens and lay a foundation for understanding associated genes and TFs regulating different pathways. Moreover, bZIP TFs may offer a comprehensive transgenic gizmo for engineering disease resistance in plant breeding programs.

Transcription factors for modification of lignin content in plants

DOEpatents

Wang, Huanzhong; Chen, Fang; Dixon, Richard A.

2015-06-02

The invention provides methods for modifying lignin, cellulose, xylan, and hemicellulose content in plants, and for achieving ectopic lignification and, for instance, secondary cell wall synthesis in pith cells, by altered regulation of a WRKY transcription factor. Nucleic acid constructs for altered WRKY-TF expression are described. Transgenic plants are provided that comprise modified pith cell walls, and lignin, cellulose, and hemicellulose content. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops.

Factors driving natural regeneration beneath a planted urban forest

Treesearch

Danica A. Doroski; Alexander J. Felson; Mark A. Bradford; Mark P. Ashton; Emily E. Oldfield; Richard A. Hallett; Sara E. Kuebbing

2018-01-01

Cities around the world are investing in urban forest plantings as a form of green infrastructure. The aim is that these plantations will develop into naturally-regenerating native forest stands. However, woody plant recruitment is often cited as the most limiting factor to creating self-sustaining urban forests. As such, there is interest in site treatments that...

A Pilot Study Investigating the Effects of Advanced Nuclear Power Plant Control Room Technologies: Methods and Qualitative Results

DOE Office of Scientific and Technical Information (OSTI.GOV)

BLanc, Katya Le; Powers, David; Joe, Jeffrey

2015-08-01

Control room modernization is an important part of life extension for the existing light water reactor fleet. None of the 99 currently operating commercial nuclear power plants in the U.S. has completed a full-scale control room modernization to date. Nuclear power plant main control rooms for the existing commercial reactor fleet remain significantly analog, with only limited digital modernizations. Upgrades in the U.S. do not achieve the full potential of newer technologies that might otherwise enhance plant and operator performance. The goal of the control room upgrade benefits research is to identify previously overlooked benefits of modernization, identify candidate technologiesmore » that may facilitate such benefits, and demonstrate these technologies through human factors research. This report describes a pilot study to test upgrades to the Human Systems Simulation Laboratory at INL.« less

Experience with ethylene plant computer control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nasi, M.; Darby, M.L.; Sourander, M.

This article discusses the control strategies, results and opinions of management and operations of a computer based ethylene plant control system. The ethylene unit contains 9 cracking heaters, and its nameplate capacity is 200,000 tpa ethylene. Reports on control performance during different unit loading and using different feedstock types. By converting the yield and utility consumption benefits due to computer control into monetary units, the payback time of the system is less than 2 yrs.

WRKY transcription factors in plant responses to stresses.

PubMed

Jiang, Jingjing; Ma, Shenghui; Ye, Nenghui; Jiang, Ming; Cao, Jiashu; Zhang, Jianhua

2017-02-01

The WRKY gene family is among the largest families of transcription factors (TFs) in higher plants. By regulating the plant hormone signal transduction pathway, these TFs play critical roles in some plant processes in response to biotic and abiotic stress. Various bodies of research have demonstrated the important biological functions of WRKY TFs in plant response to different kinds of biotic and abiotic stresses and working mechanisms. However, very little summarization has been done to review their research progress. Not just important TFs function in plant response to biotic and abiotic stresses, WRKY also participates in carbohydrate synthesis, senescence, development, and secondary metabolites synthesis. WRKY proteins can bind to W-box (TGACC (A/T)) in the promoter of its target genes and activate or repress the expression of downstream genes to regulate their stress response. Moreover, WRKY proteins can interact with other TFs to regulate plant defensive responses. In the present review, we focus on the structural characteristics of WRKY TFs and the research progress on their functions in plant responses to a variety of stresses. © 2016 Institute of Botany, Chinese Academy of Sciences.

Role of Homeodomain leucine zipper (HD-Zip) IV transcription factors in plant development and plant protection from deleterious environmental factors.

PubMed

Chew, William; Hrmova, Maria; Lopato, Sergiy

2013-04-12

Homeobox genes comprise an important group of genes that are responsible for regulation of developmental processes. These genes determine cell differentiation and cell fate in all eukaryotic organisms, starting from the early stages of embryo development. Homeodomain leucine zipper (HD-Zip) transcription factors are unique to the plant kingdom. Members of the HD-Zip IV subfamily have a complex domain topology and can bind several cis-elements with overlapping sequences. Many of the reported HD-Zip IV genes were shown to be specifically or preferentially expressed in plant epidermal or sub-epidermal cells. HD-Zip IV TFs were found to be associated with differentiation and maintenance of outer cell layers, and regulation of lipid biosynthesis and transport. Insights about the role of these proteins in plant cuticle formation, and hence their possible involvement in plant protection from pathogens and abiotic stresses has just started to emerge. These roles make HD-Zip IV proteins an attractive tool for genetic engineering of crop plants. To this end, there is a need for in-depth studies to further clarify the function of each HD-Zip IV subfamily member in commercially important plant species.

Role of Homeodomain Leucine Zipper (HD-Zip) IV Transcription Factors in Plant Development and Plant Protection from Deleterious Environmental Factors

PubMed Central

Chew, William; Hrmova, Maria; Lopato, Sergiy

2013-01-01

Homeobox genes comprise an important group of genes that are responsible for regulation of developmental processes. These genes determine cell differentiation and cell fate in all eukaryotic organisms, starting from the early stages of embryo development. Homeodomain leucine zipper (HD-Zip) transcription factors are unique to the plant kingdom. Members of the HD-Zip IV subfamily have a complex domain topology and can bind several cis-elements with overlapping sequences. Many of the reported HD-Zip IV genes were shown to be specifically or preferentially expressed in plant epidermal or sub-epidermal cells. HD-Zip IV TFs were found to be associated with differentiation and maintenance of outer cell layers, and regulation of lipid biosynthesis and transport. Insights about the role of these proteins in plant cuticle formation, and hence their possible involvement in plant protection from pathogens and abiotic stresses has just started to emerge. These roles make HD-Zip IV proteins an attractive tool for genetic engineering of crop plants. To this end, there is a need for in-depth studies to further clarify the function of each HD-Zip IV subfamily member in commercially important plant species. PMID:23584027

Factors affecting plant growth in membrane nutrient delivery

NASA Technical Reports Server (NTRS)

Dreschel, T. W.; Wheeler, R. M.; Sager, J. C.; Knott, W. M.

1990-01-01

The development of the tubular membrane plant growth unit for the delivery of water and nutrients to roots in microgravity has recently focused on measuring the effects of changes in physical variables controlling solution availability to the plants. Significant effects of membrane pore size and the negative pressure used to contain the solution were demonstrated. Generally, wheat grew better in units with a larger pore size but equal negative pressure and in units with the same pore size but less negative pressure. Lettuce also exhibited better plant growth at less negative pressure.

Development of model reference adaptive control theory for electric power plant control applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mabius, L.E.

1982-09-15

The scope of this effort includes the theoretical development of a multi-input, multi-output (MIMO) Model Reference Control (MRC) algorithm, (i.e., model following control law), Model Reference Adaptive Control (MRAC) algorithm and the formulation of a nonlinear model of a typical electric power plant. Previous single-input, single-output MRAC algorithm designs have been generalized to MIMO MRAC designs using the MIMO MRC algorithm. This MRC algorithm, which has been developed using Command Generator Tracker methodologies, represents the steady state behavior (in the adaptive sense) of the MRAC algorithm. The MRC algorithm is a fundamental component in the MRAC design and stability analysis.more » An enhanced MRC algorithm, which has been developed for systems with more controls than regulated outputs, alleviates the MRC stability constraint of stable plant transmission zeroes. The nonlinear power plant model is based on the Cromby model with the addition of a governor valve management algorithm, turbine dynamics and turbine interactions with extraction flows. An application of the MRC algorithm to a linearization of this model demonstrates its applicability to power plant systems. In particular, the generated power changes at 7% per minute while throttle pressure and temperature, reheat temperature and drum level are held constant with a reasonable level of control. The enhanced algorithm reduces significantly control fluctuations without modifying the output response.« less

Fuzzy Logic Based Controller for a Grid-Connected Solid Oxide Fuel Cell Power Plant.

PubMed

Chatterjee, Kalyan; Shankar, Ravi; Kumar, Amit

2014-10-01

This paper describes a mathematical model of a solid oxide fuel cell (SOFC) power plant integrated in a multimachine power system. The utilization factor of a fuel stack maintains steady state by tuning the fuel valve in the fuel processor at a rate proportional to a current drawn from the fuel stack. A suitable fuzzy logic control is used for the overall system, its objective being controlling the current drawn by the power conditioning unit and meet a desirable output power demand. The proposed control scheme is verified through computer simulations.

Wind Plant Power Optimization and Control under Uncertainty

NASA Astrophysics Data System (ADS)

Jha, Pankaj; Ulker, Demet; Hutchings, Kyle; Oxley, Gregory

2017-11-01

The development of optimized cooperative wind plant control involves the coordinated operation of individual turbines co-located within a wind plant to improve the overall power production. This is typically achieved by manipulating the trajectory and intensity of wake interactions between nearby turbines, thereby reducing wake losses. However, there are various types of uncertainties involved, such as turbulent inflow and microscale and turbine model input parameters. In a recent NREL-Envision collaboration, a controller that performs wake steering was designed and implemented for the Longyuan Rudong offshore wind plant in Jiangsu, China. The Rudong site contains 25 Envision EN136-4 MW turbines, of which a subset was selected for the field test campaign consisting of the front two rows for the northeasterly wind direction. In the first row, a turbine was selected as the reference turbine, providing comparison power data, while another was selected as the controlled turbine. This controlled turbine wakes three different turbines in the second row depending on the wind direction. A yaw misalignment strategy was designed using Envision's GWCFD, a multi-fidelity plant-scale CFD tool based on SOWFA with a generalized actuator disc (GAD) turbine model, which, in turn, was used to tune NREL's FLORIS model used for wake steering and yaw control optimization. The presentation will account for some associated uncertainties, such as those in atmospheric turbulence and wake profile.

Inhibitory Effects of Bangladeshi Medicinal Plant Extracts on Interactions between Transcription Factors and Target DNA Sequences

PubMed Central

Lampronti, Ilaria; Khan, Mahmud T.H.; Borgatti, Monica; Bianchi, Nicoletta

2008-01-01

Several transcription factors (TFs) play crucial roles in governing the expression of different genes involved in the immune response, embryo or cell lineage development, cell apoptosis, cell cycle progression, oncogenesis, repair and fibrosis processes and inflammation. As far as inflammation, TFs playing pivotal roles are nuclear factor kappa B (NF-kB), activator protein (AP-1), signal transducer and activator of transcription (STATs), cAMP response element binding protein (CREB) and GATA-1 factors. All these TFs regulate the expression of pro-inflammatory cytokines and are involved in the pathogenesis of a number of human disorders, particularly those with an inflammatory component. Since several medicinal plants can be employed to produce extracts exhibiting biological effects and because alteration of gene transcription represents a very interesting approach to control the expression of selected genes, this study sought to verify the ability of several extracts derived from Bangladeshi medicinal plants in interfering with molecular interactions between different TFs and specific DNA sequences. We first analyzed the antiproliferative activity of 19 medicinal plants on different human cell lines, including erythroleukemia K562, B lymphoid Raji and T lymphoid Jurkat cell lines. Secondly, we employed the electrophoretic mobility shift assay as a suitable technique for a fast screening of plant extracts altering the binding between NF-kB, AP-1, GATA-1, STAT-3, CREB and the relative target DNA elements. PMID:18830455

Control of trichome formation in Arabidopsis by poplar single-repeat R3 MYB transcription factors

PubMed Central

Zhou, Limei; Zheng, Kaijie; Wang, Xiaoyu; Tian, Hainan; Wang, Xianling; Wang, Shucai

2014-01-01

In Arabidopsis, trichome formation is regulated by the interplay of R3 MYBs and several others transcription factors including the WD40-repeat protein TRANSPARENT TESTA GLABRA1 (TTG1), the R2R3 MYB transcription factor GLABRA1 (GL1), the bHLH transcription factor GLABRA3 (GL3) or ENHANCER OF GLABRA3 (EGL3), and the homeodomain protein GLABRA2 (GL2). R3 MYBs including TRICHOMELESS1 (TCL1), TCL2, TRYPTICHON (TRY), CAPRICE (CPC), ENHANCER OF TRY AND CPC1 (ETC1), ETC2 and ETC3 negatively regulate trichome formation by competing with GL1 for binding GL3 or EGL3, thus blocking the formation of TTG1–GL3/EGL3–GL1, an activator complex required for the activation of the trichome positive regulator gene GL2. However, it is largely unknown if R3 MYBs in other plant species especially woody plants have similar functions. By BLASTing the Populus trichocarpa protein database using the entire amino acid sequence of TCL1, an Arabidopsis R3 MYB transcription factor, we identified a total of eight R3 MYB transcription factor genes in poplar, namely P. trichocarpa TRICHOMELESS1 through 8 (PtrTCL1–PtrTCL8). The amino acid signature required for interacting with bHLH transcription factors and the amino acids required for cell-to-cell movement of R3 MYBs are not fully conserved in all PtrTCLs. When tested in Arabidopsis protoplasts, however, all PtrTCLs interacted with GL3. Expressing each of the eight PtrTCL genes in Arabidopsis resulted in either glabrous phenotypes or plants with reduced trichome numbers, and expression levels of GL2 in all transgenic plants tested were greatly reduced. Expression of PtrTCL1 under the control of TCL1 native promoter almost completely complemented the mutant phenotype of tcl. In contrast, expression of PtrTCL1 under the control of TRY native promoter in the try mutant, or under the control of CPC native promoter in the cpc mutant resulted in glabrous phenotypes, suggesting that PtrTCL1 functions similarly to TCL1, but not TRY and CPC. PMID

Control oriented concentrating solar power (CSP) plant model and its applications

NASA Astrophysics Data System (ADS)

Luo, Qi

Solar receivers in concentrating solar thermal power plants (CSP) undergo over 10,000 start-ups and shutdowns, and over 25,000 rapid rate of change in temperature on receivers due to cloud transients resulting in performance degradation and material fatigue in their expected lifetime of over 30 years. The research proposes to develop a three-level controller that uses multi-input-multi-output (MIMO) control technology to minimize the effect of these disturbances, improve plant performance, and extend plant life. The controller can be readily installed on any vendor supplied state-of-the-art control hardware. We propose a three-level controller architecture using multi-input-multi-output (MIMO) control for CSP plants that can be implemented on existing plants to improve performance, reliability, and extend the life of the plant. This architecture optimizes the performance on multiple time scalesreactive level (regulation to temperature set points), tactical level (adaptation of temperature set points), and strategic level (trading off fatigue life due to thermal cycling and current production). This controller unique to CSP plants operating at temperatures greater than 550 °C, will make CSPs competitive with conventional power plants and contribute significantly towards the Sunshot goal of 0.06/kWh(e), while responding with agility to both market dynamics and changes in solar irradiance such as due to passing clouds. Moreover, our development of control software with performance guarantees will avoid early stage failures and permit smooth grid integration of the CSP power plants. The proposed controller can be implemented with existing control hardware infrastructure with little or no additional equipment. In the thesis, we demonstrate a dynamics model of CSP, of which different components are modelled with different time scales. We also show a real time control strategy of CSP control oriented model in steady state. Furthermore, we shown different controllers

[Introduction of hazard analysis and critical control points (HACCP) principles at the flight catering food production plant].

PubMed

Popova, A Yu; Trukhina, G M; Mikailova, O M

In the article there is considered the quality control and safety system implemented in the one of the largest flight catering food production plant for airline passengers and flying squad. The system for the control was based on the Hazard Analysis And Critical Control Points (HACCP) principles and developed hygienic and antiepidemic measures. There is considered the identification of hazard factors at stages of the technical process. There are presented results of the analysis data of monitoring for 6 critical control points over the five-year period. The quality control and safety system permit to decline food contamination risk during acceptance, preparation and supplying of in-flight meal. There was proved the efficiency of the implemented system. There are determined further ways of harmonization and implementation for HACCP principles in the plant.

7. WEYMOUTH FILTRATION PLANT, BUILDING 1 INTERIOR: LA VERNE CONTROL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. WEYMOUTH FILTRATION PLANT, BUILDING 1 INTERIOR: LA VERNE CONTROL ROOM, REGULATES DISTRIBUTION OF WATER, CONTROLS POWER HOUSES. - F. E. Weymouth Filtration Plant, 700 North Moreno Avenue, La Verne, Los Angeles County, CA

Analysis of axial-induction-based wind plant control using an engineering and a high-order wind plant model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Annoni, Jennifer; Gebraad, Pieter M. O.; Scholbrock, Andrew K.

2015-08-14

Wind turbines are typically operated to maximize their performance without considering the impact of wake effects on nearby turbines. Wind plant control concepts aim to increase overall wind plant performance by coordinating the operation of the turbines. This paper focuses on axial-induction-based wind plant control techniques, in which the generator torque or blade pitch degrees of freedom of the wind turbines are adjusted. The paper addresses discrepancies between a high-order wind plant model and an engineering wind plant model. Changes in the engineering model are proposed to better capture the effects of axial-induction-based control shown in the high-order model.

[Analysis of related factors of slope plant hyperspectral remote sensing].

PubMed

Sun, Wei-Qi; Zhao, Yun-Sheng; Tu, Lin-Ling

2014-09-01

In the present paper, the slope gradient, aspect, detection zenith angle and plant types were analyzed. In order to strengthen the theoretical discussion, the research was under laboratory condition, and modeled uniform slope for slope plant. Through experiments we found that these factors indeed have influence on plant hyperspectral remote sensing. When choosing slope gradient as the variate, the blade reflection first increases and then decreases as the slope gradient changes from 0° to 36°; When keeping other factors constant, and only detection zenith angle increasing from 0° to 60°, the spectral characteristic of slope plants do not change significantly in visible light band, but decreases gradually in near infrared band; With only slope aspect changing, when the dome meets the light direction, the blade reflectance gets maximum, and when the dome meets the backlit direction, the blade reflectance gets minimum, furthermore, setting the line of vertical intersection of incidence plane and the dome as an axis, the reflectance on the axis's both sides shows symmetric distribution; In addition, spectral curves of different plant types have a lot differences between each other, which means that the plant types also affect hyperspectral remote sensing results of slope plants. This research breaks through the limitations of the traditional vertical remote sensing data collection and uses the multi-angle and hyperspectral information to analyze spectral characteristics of slope plants. So this research has theoretical significance to the development of quantitative remote sensing, and has application value to the plant remote sensing monitoring.

Can mechanics control pattern formation in plants?

PubMed

Dumais, Jacques

2007-02-01

Development of the plant body entails many pattern forming events at scales ranging from the cellular level to the whole plant. Recent evidence suggests that mechanical forces play a role in establishing some of these patterns. The development of cellular configurations in glandular trichomes and the rippling of leaf surfaces are discussed in depth to illustrate how intricate patterns can emerge from simple and well-established molecular and cellular processes. The ability of plants to sense and transduce mechanical signals suggests that complex interactions between mechanics and chemistry are possible during plant development. The inclusion of mechanics alongside traditional molecular controls offers a more comprehensive view of developmental processes.

Optimization control of LNG regasification plant using Model Predictive Control

NASA Astrophysics Data System (ADS)

Wahid, A.; Adicandra, F. F.

2018-03-01

Optimization of liquified natural gas (LNG) regasification plant is important to minimize costs, especially operational costs. Therefore, it is important to choose optimum LNG regasification plant design and maintaining the optimum operating conditions through the implementation of model predictive control (MPC). Optimal tuning parameter for MPC such as P (prediction horizon), M (control of the horizon) and T (sampling time) are achieved by using fine-tuning method. The optimal criterion for design is the minimum amount of energy used and for control is integral of square error (ISE). As a result, the optimum design is scheme 2 which is developed by Devold with an energy savings of 40%. To maintain the optimum conditions, required MPC with P, M and T as follows: tank storage pressure: 90, 2, 1; product pressure: 95, 2, 1; temperature vaporizer: 65, 2, 2; and temperature heater: 35, 6, 5, with ISE value at set point tracking respectively 0.99, 1792.78, 34.89 and 7.54, or improvement of control performance respectively 4.6%, 63.5%, 3.1% and 58.2% compared to PI controller performance. The energy savings that MPC controllers can make when there is a disturbance in temperature rise 1°C of sea water is 0.02 MW.

Nice to meet you: genetic, epigenetic and metabolic controls of plant perception of beneficial associative and endophytic diazotrophic bacteria in non-leguminous plants.

PubMed

Carvalho, T L G; Ballesteros, H G F; Thiebaut, F; Ferreira, P C G; Hemerly, A S

2016-04-01

A wide range of rhizosphere diazotrophic bacteria are able to establish beneficial associations with plants, being able to associate to root surfaces or even endophytically colonize plant tissues. In common, both associative and endophytic types of colonization can result in beneficial outcomes to the plant leading to plant growth promotion, as well as increase in tolerance against biotic and abiotic stresses. An intriguing question in such associations is how plant cell surface perceives signals from other living organisms, thus sorting pathogens from beneficial ones, to transduce this information and activate proper responses that will finally culminate in plant adaptations to optimize their growth rates. This review focuses on the recent advances in the understanding of genetic and epigenetic controls of plant-bacteria signaling and recognition during beneficial associations with associative and endophytic diazotrophic bacteria. Finally, we propose that "soil-rhizosphere-rhizoplane-endophytes-plant" could be considered as a single coordinated unit with dynamic components that integrate the plant with the environment to generate adaptive responses in plants to improve growth. The homeostasis of the whole system should recruit different levels of regulation, and recognition between the parties in a given environment might be one of the crucial factors coordinating these adaptive plant responses.

DOSE CONTROLLER FOR AGUACLARA WATER TREATMENT PLANTS

EPA Science Inventory

The expected results include a proven design for a gravity powered dose controller that works for calcium hypochlorite or aluminum sulfate solutions. The dose controller will be coupled with plant flow rate measuring systems that have compatible relationships between flow rate...

Plant diversity drives soil microbial biomass carbon in grasslands irrespective of global environmental change factors.

PubMed

Thakur, Madhav Prakash; Milcu, Alexandru; Manning, Pete; Niklaus, Pascal A; Roscher, Christiane; Power, Sally; Reich, Peter B; Scheu, Stefan; Tilman, David; Ai, Fuxun; Guo, Hongyan; Ji, Rong; Pierce, Sarah; Ramirez, Nathaly Guerrero; Richter, Annabell Nicola; Steinauer, Katja; Strecker, Tanja; Vogel, Anja; Eisenhauer, Nico

2015-11-01

Soil microbial biomass is a key determinant of carbon dynamics in the soil. Several studies have shown that soil microbial biomass significantly increases with plant species diversity, but it remains unclear whether plant species diversity can also stabilize soil microbial biomass in a changing environment. This question is particularly relevant as many global environmental change (GEC) factors, such as drought and nutrient enrichment, have been shown to reduce soil microbial biomass. Experiments with orthogonal manipulations of plant diversity and GEC factors can provide insights whether plant diversity can attenuate such detrimental effects on soil microbial biomass. Here, we present the analysis of 12 different studies with 14 unique orthogonal plant diversity × GEC manipulations in grasslands, where plant diversity and at least one GEC factor (elevated CO2 , nutrient enrichment, drought, earthworm presence, or warming) were manipulated. Our results show that higher plant diversity significantly enhances soil microbial biomass with the strongest effects in long-term field experiments. In contrast, GEC factors had inconsistent effects with only drought having a significant negative effect. Importantly, we report consistent non-significant effects for all 14 interactions between plant diversity and GEC factors, which indicates a limited potential of plant diversity to attenuate the effects of GEC factors on soil microbial biomass. We highlight that plant diversity is a major determinant of soil microbial biomass in experimental grasslands that can influence soil carbon dynamics irrespective of GEC. © 2015 John Wiley & Sons Ltd.

Computational Fluid Dynamics Simulation Study of Active Power Control in Wind Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fleming, Paul; Aho, Jake; Gebraad, Pieter

2016-08-01

This paper presents an analysis performed on a wind plant's ability to provide active power control services using a high-fidelity computational fluid dynamics-based wind plant simulator. This approach allows examination of the impact on wind turbine wake interactions within a wind plant on performance of the wind plant controller. The paper investigates several control methods for improving performance in waked conditions. One method uses wind plant wake controls, an active field of research in which wind turbine control systems are coordinated to account for their wakes, to improve the overall performance. Results demonstrate the challenge of providing active power controlmore » in waked conditions but also the potential methods for improving this performance.« less

Plant growth chamber based on space proven controlled environment technology

NASA Astrophysics Data System (ADS)

Ignatius, Ronald W.; Ignatius, Matt H.; Imberti, Henry J.

1997-01-01

Quantum Devices, Inc., in conjunction with Percival Scientific, Inc., and the Wisconsin Center for Space Automation and Robotics (WCSAR) have developed a controlled environment plant growth chamber for terrestrial agricultural and scientific applications. This chamber incorporates controlled environment technology used in the WCSAR ASTROCULTURE™ flight unit for conducting plant research on the Space Shuttle. The new chamber, termed CERES 2010, features air humidity, temperature, and carbon dioxide control, an atmospheric contaminant removal unit, an LED lighting system, and a water and nutrient delivery system. The advanced environment control technology used in this chamber will increase the reliability and repeatability of environmental physiology data derived from plant experiments conducted in this chamber.

Development of Plant Control Diagnosis Technology and Increasing Its Applications

NASA Astrophysics Data System (ADS)

Kugemoto, Hidekazu; Yoshimura, Satoshi; Hashizume, Satoru; Kageyama, Takashi; Yamamoto, Toru

A plant control diagnosis technology was developed to improve the performance of plant-wide control and maintain high productivity of plants. The control performance diagnosis system containing this technology picks out the poor performance loop, analyzes the cause, and outputs the result on the Web page. Meanwhile, the PID tuning tool is used to tune extracted loops from the control performance diagnosis system. It has an advantage of tuning safely without process changes. These systems are powerful tools to do Kaizen (continuous improvement efforts) step by step, coordinating with the operator. This paper describes a practical technique regarding the diagnosis system and its industrial applications.

Controlled activation of retrotransposition for plant breeding.

PubMed

Paszkowski, Jerzy

2015-04-01

Plant genomes consist to a large extent of transposable elements (TEs), predominantly retrotransposons. Their accumulation through periodic transposition bursts has shaped the structure and regulatory organization of plant genomes, often contributing to phenotypic traits. Transposon-generated phenotypes selected by humans during plant domestication have been maintained under strict selection during subsequent plant breeding. Our knowledge of the epigenetic, environmental, and developmental regulation of TE activity has advanced considerably in recent years. Here I will consider TEs as an attractive endogenous source of genetic variation that can be liberated in a controlled fashion and thus offer novel phenotypic diversity to be selected for crop improvement. Copyright © 2015 Elsevier Ltd. All rights reserved.

Optimization of controlled processes in combined-cycle plant (new developments and researches)

NASA Astrophysics Data System (ADS)

Tverskoy, Yu S.; Muravev, I. K.

2017-11-01

All modern complex technical systems, including power units of TPP and nuclear power plants, work in the system-forming structure of multifunctional APCS. The development of the modern APCS mathematical support allows bringing the automation degree to the solution of complex optimization problems of equipment heat-mass-exchange processes in real time. The difficulty of efficient management of a binary power unit is related to the need to solve jointly at least three problems. The first problem is related to the physical issues of combined-cycle technologies. The second problem is determined by the criticality of the CCGT operation to changes in the regime and climatic factors. The third problem is related to a precise description of a vector of controlled coordinates of a complex technological object. To obtain a joint solution of this complex of interconnected problems, the methodology of generalized thermodynamic analysis, methods of the theory of automatic control and mathematical modeling are used. In the present report, results of new developments and studies are shown. These results allow improving the principles of process control and the automatic control systems structural synthesis of power units with combined-cycle plants that provide attainable technical and economic efficiency and operational reliability of equipment.

Controlling exotic plants in your forest

Treesearch

James H. Miller

1999-01-01

The author discusses the impacts of exotic plants and suggests control and rehabilitation measures. Trees, shrubs, and vines addressed include silk tree or mimosa, Chinese and Japanese privet, kudzu, multiflora rose, Japanese honeysuckle, and Chinese wisteria.

The Effects of Abiotic Factors on Induced Volatile Emissions in Corn Plants1

PubMed Central

Gouinguené, Sandrine P.; Turlings, Ted C.J.

2002-01-01

Many plants respond to herbivory by releasing a specific blend of volatiles that is attractive to natural enemies of the herbivores. In corn (Zea mays), this induced odor blend is mainly composed of terpenoids and indole. The induced signal varies with plant species and genotype, but little is known about the variation due to abiotic factors. Here, we tested the effect of soil humidity, air humidity, temperature, light, and fertilization rate on the emission of induced volatiles in young corn plants. Each factor was tested separately under constant conditions for the other factors. Plants released more when standing in dry soil than in wet soil, whereas for air humidity, the optimal release was found at around 60% relative humidity. Temperatures between 22°C and 27°C led to a higher emission than lower or higher temperatures. Light intensity had a dramatic effect. The emission of volatiles did not occur in the dark and increased steadily with an increase in the light intensity. An experiment with an unnatural light-dark cycle showed that the release was fully photophase dependent. Fertilization also had a strong positive effect; the emission of volatiles was minimal when plants were grown under low nutrition, even when results were corrected for plant biomass. Changes in all abiotic factors caused small but significant changes in the relative ratios among the different compounds (quality) in the induced odor blends, except for air humidity. Hence, climatic conditions and nutrient availability can be important factors in determining the intensity and variability in the release of induced plant volatiles. PMID:12114583

SSD1, which encodes a plant-specific novel protein, controls plant elongation by regulating cell division in rice.

PubMed

Asano, Kenji; Miyao, Akio; Hirochika, Hirohiko; Kitano, Hidemi; Matsuoka, Makoto; Ashikari, Motoyuki

2010-01-01

Plant height is one of the most important traits in crop improvement. Therefore revealing the mechanism of plant elongation and controlling plant height in accordance with breeding object is important. In this study we analyzed a novel dwarf mutant, ssd1, of which phenotype is different from typical GA- or BR-related dwarf phenotype. ssd1 exhibits pleiotropic defects in elongation of various organs such as stems, roots, leaves, and flowers. ssd1 also shows abnormal cell files and shapes, which suggests defects of normal cell division in the mutant. Map-based cloning and complementation test demonstrated that the dwarf phenotype in ssd1 mutant was caused by insertion of retrotransposon in a gene, which encodes plant-specific protein with unknown biochemical function. A BLAST search revealed that SSD1-like genes exist in diverse plant species, including monocots and dicots, but not fern and moss. Our results demonstrate that SSD1 controls plant elongation by controlling cell division in higher plants.

Plant-centered biosystems in space environments: technological concepts for developing a plant genetic assessment and control system.

PubMed

Lomax, Terri L; Findlay, Kirk A; White, T J; Winner, William E

2003-06-01

Plants will play an essential role in providing life support for any long-term space exploration or habitation. We are evaluating the feasibility of an adaptable system for measuring the response of plants to any unique space condition and optimizing plant performance under those conditions. The proposed system is based on a unique combination of systems including the rapid advances in the field of plant genomics, microarray technology for measuring gene expression, bioinformatics, gene pathways and networks, physiological measurements in controlled environments, and advances in automation and robotics. The resulting flexible module for monitoring and optimizing plant responses will be able to be inserted as a cassette into a variety of platforms and missions for either experimental or life support purposes. The results from future plant functional genomics projects have great potential to be applied to those plant species most likely to be used in space environments. Eventually, it will be possible to use the plant genetic assessment and control system to optimize the performance of any plant in any space environment. In addition to allowing the effective control of environmental parameters for enhanced plant productivity and other life support functions, the proposed module will also allow the selection or engineering of plants to thrive in specific space environments. The proposed project will advance human exploration of space in the near- and mid-term future on the International Space Station and free-flying satellites and in the far-term for longer duration missions and eventual space habitation.

Chapter 8. Controlling plant competition

Treesearch

Stephen B. Monsen

2004-01-01

Generally, range or wildlife habitat improvement projects seek to achieve desirable plants through the elimination or replacement of undesirable species or both. Control measures are thus designed to: (1) reduce the competitive effects of existing species (Evans and Young 1987a,b; Robertson and Pearse 1945), (2) allow the establishment of seeded species (Harper and...

Jasmonate-responsive transcription factors regulating plant secondary metabolism.

PubMed

Zhou, Meiliang; Memelink, Johan

2016-01-01

Plants produce a large variety of secondary metabolites including alkaloids, glucosinolates, terpenoids and phenylpropanoids. These compounds play key roles in plant-environment interactions and many of them have pharmacological activity in humans. Jasmonates (JAs) are plant hormones which induce biosynthesis of many secondary metabolites. JAs-responsive transcription factors (TFs) that regulate the JAs-induced accumulation of secondary metabolites belong to different families including AP2/ERF, bHLH, MYB and WRKY. Here, we give an overview of the types and functions of TFs that have been identified in JAs-induced secondary metabolite biosynthesis, and highlight their similarities and differences in regulating various biosynthetic pathways. We review major recent developments regarding JAs-responsive TFs mediating secondary metabolite biosynthesis, and provide suggestions for further studies. Copyright © 2016 Elsevier Inc. All rights reserved.

Sucrose-induced anthocyanin accumulation in vegetative tissue of Petunia plants requires anthocyanin regulatory transcription factors.

PubMed

Ai, Trinh Ngoc; Naing, Aung Htay; Arun, Muthukrishnan; Lim, Sun-Hyung; Kim, Chang Kil

2016-11-01

The effects of three different sucrose concentrations on plant growth and anthocyanin accumulation were examined in non-transgenic (NT) and transgenic (T 2 ) specimens of the Petunia hybrida cultivar 'Mirage rose' that carried the anthocyanin regulatory transcription factors B-Peru+mPAP1 or RsMYB1. Anthocyanin accumulation was not observed in NT plants in any treatments, whereas a range of anthocyanin accumulation was observed in transgenic plants. The anthocyanin content detected in transgenic plants expressing the anthocyanin regulatory transcription factors (B-Peru+mPAP1 or RsMYB1) was higher than that in NT plants. In addition, increasing sucrose concentration strongly enhanced anthocyanin content as shown by quantitative real-time polymerase chain reaction (qRT-PCR) analysis, wherein increased concentrations of sucrose enhanced transcript levels of the transcription factors that are responsible for the induction of biosynthetic genes involved in anthocyanin synthesis; this pattern was not observed in NT plants. In addition, sucrose affected plant growth, although the effects were different between NT and transgenic plants. Taken together, the application of sucrose could enhance anthocyanin production in vegetative tissue of transgenic Petunia carrying anthocyanin regulatory transcription factors, and this study provides insights about interactive effects of sucrose and transcription factors in anthocyanin biosynthesis in the transgenic plant. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Variations of Histone Modification Patterns: Contributions of Inter-plant Variability and Technical Factors

PubMed Central

Brabencová, Sylva; Ihnatová, Ivana; Potěšil, David; Fojtová, Miloslava; Fajkus, Jiří; Zdráhal, Zbyněk; Lochmanová, Gabriela

2017-01-01

Inter-individual variability of conspecific plants is governed by differences in their genetically determined growth and development traits, environmental conditions, and adaptive responses under epigenetic control involving histone post-translational modifications. The apparent variability in histone modifications among plants might be increased by technical variation introduced in sample processing during epigenetic analyses. Thus, to detect true variations in epigenetic histone patterns associated with given factors, the basal variability among samples that is not associated with them must be estimated. To improve knowledge of relative contribution of biological and technical variation, mass spectrometry was used to examine histone modification patterns (acetylation and methylation) among Arabidopsis thaliana plants of ecotypes Columbia 0 (Col-0) and Wassilewskija (Ws) homogenized by two techniques (grinding in a cryomill or with a mortar and pestle). We found little difference in histone modification profiles between the ecotypes. However, in comparison of the biological and technical components of variability, we found consistently higher inter-individual variability in histone mark levels among Ws plants than among Col-0 plants (grown from seeds collected either from single plants or sets of plants). Thus, more replicates of Ws would be needed for rigorous analysis of epigenetic marks. Regarding technical variability, the cryomill introduced detectably more heterogeneity in the data than the mortar and pestle treatment, but mass spectrometric analyses had minor apparent effects. Our study shows that it is essential to consider inter-sample variance and estimate suitable numbers of biological replicates for statistical analysis for each studied organism when investigating changes in epigenetic histone profiles. PMID:29270186

Variations of Histone Modification Patterns: Contributions of Inter-plant Variability and Technical Factors.

PubMed

Brabencová, Sylva; Ihnatová, Ivana; Potěšil, David; Fojtová, Miloslava; Fajkus, Jiří; Zdráhal, Zbyněk; Lochmanová, Gabriela

2017-01-01

Inter-individual variability of conspecific plants is governed by differences in their genetically determined growth and development traits, environmental conditions, and adaptive responses under epigenetic control involving histone post-translational modifications. The apparent variability in histone modifications among plants might be increased by technical variation introduced in sample processing during epigenetic analyses. Thus, to detect true variations in epigenetic histone patterns associated with given factors, the basal variability among samples that is not associated with them must be estimated. To improve knowledge of relative contribution of biological and technical variation, mass spectrometry was used to examine histone modification patterns (acetylation and methylation) among Arabidopsis thaliana plants of ecotypes Columbia 0 (Col-0) and Wassilewskija (Ws) homogenized by two techniques (grinding in a cryomill or with a mortar and pestle). We found little difference in histone modification profiles between the ecotypes. However, in comparison of the biological and technical components of variability, we found consistently higher inter-individual variability in histone mark levels among Ws plants than among Col-0 plants (grown from seeds collected either from single plants or sets of plants). Thus, more replicates of Ws would be needed for rigorous analysis of epigenetic marks. Regarding technical variability, the cryomill introduced detectably more heterogeneity in the data than the mortar and pestle treatment, but mass spectrometric analyses had minor apparent effects. Our study shows that it is essential to consider inter-sample variance and estimate suitable numbers of biological replicates for statistical analysis for each studied organism when investigating changes in epigenetic histone profiles.

Transcription Factors and Their Roles in Signal Transduction in Plants under Abiotic Stresses

PubMed Central

Hoang, Xuan Lan Thi; Nhi, Du Ngoc Hai; Thu, Nguyen Binh Anh; Thao, Nguyen Phuong; Tran, Lam-Son Phan

2017-01-01

Abstract: In agricultural production, abiotic stresses are known as the main disturbance leading to negative impacts on crop performance. Research on elucidating plant defense mechanisms against the stresses at molecular level has been addressed for years in order to identify the major contributors in boosting the plant tolerance ability. From literature, numerous genes from different species, and from both functional and regulatory gene categories, have been suggested to be on the list of potential candidates for genetic engineering. Noticeably, enhancement of plant stress tolerance by manipulating expression of Transcription Factors (TFs) encoding genes has emerged as a popular approach since most of them are early stress-responsive genes and control the expression of a set of downstream target genes. Consequently, there is a higher chance to generate novel cultivars with better tolerance to either single or multiple stresses. Perhaps, the difficult task when deploying this approach is selecting appropriate gene(s) for manipulation. In this review, on the basis of the current findings from molecular and post-genomic studies, our interest is to highlight the current understanding of the roles of TFs in signal transduction and mediating plant responses towards abiotic stressors. Furthermore, interactions among TFs within the stress-responsive network will be discussed. The last section will be reserved for discussing the potential applications of TFs for stress tolerance improvement in plants. PMID:29204078

Can plant-natural enemy communication withstand disruption by biotic and abiotic factors?

PubMed

Clavijo McCormick, Andrea

2016-12-01

The attraction of natural enemies towards herbivore-induced plant volatiles is a well-documented phenomenon. However, the majority of published studies are carried under optimal water and nutrient regimes and with just one herbivore. But what happens when additional levels of ecological complexity are added? Does the presence of a second herbivore, microorganisms, and abiotic stress interfere with plant-natural enemy communication? or is communication stable enough to withstand disruption by additional biotic and abiotic factors?Investigating the effects of these additional levels of ecological complexity is key to understanding the stability of tritrophic interactions in natural ecosystems and may aid to forecast the impact of environmental disturbances on these, especially in climate change scenarios, which are often associated with modifications in plant and arthropod species distribution and increased levels of abiotic stress.This review explores the literature on natural enemy attraction to herbivore-induced volatiles when, besides herbivory, plants are challenged by additional biotic and abiotic factors.The aim of this review was to establish the impact of different biotic and abiotic factors on plant-natural enemy communication and to highlight critical aspects to guide future research efforts.

Systemic properties of myclobutanil in soybean plants, affecting control of Asian soybean rust (Phakopsora pachyrhizi).

PubMed

Kemmitt, Gregory M; DeBoer, Gerrit; Ouimette, David; Iamauti, Marilene

2008-12-01

The demethylation inhibitor (DMI) fungicide myclobutanil can be an effective component of spray programmes designed to control the highly destructive plant pathogen Phakopsora pachyrhizi Syd. & P. Syd., causal agent of Asian soybean rust. Myclobutanil is known from previous studies in grapevines to be xylem mobile. This study investigates the mobility profile of myclobutanil in soybean as an important component of its effective field performance. Over a 12 day period under greenhouse conditions, a constant uptake of myclobutanil from leaflet surfaces into the leaflet tissue was observed. Once in the leaflet, myclobutanil was seen to redistribute throughout the tissue, although no movement out of leaflets occurred owing to a lack of phloem mobility. The ability of myclobutanil to redistribute over distance within the soybean plant was revealed when visualizing movement of the compound to foliage above the point of application on the plant stem. An efficacy bioassay demonstrated that the systemic properties of myclobutanil allow control of disease at a point remote from the initial site of compound application. It is suggested that the high degree of xylem systemicity displayed by myclobutanil in soybean foliage is a contributory factor towards its commercial effectiveness for control of Asian soybean rust.

Gibberellin in plant height control: old player, new story.

PubMed

Wang, Yijun; Zhao, Jia; Lu, Wenjie; Deng, Dexiang

2017-03-01

Height relates to plant architecture, lodging resistance, and yield performance. Growth-promoting phytohormones gibberellins (GAs) play a pivotal role in plant height control. Mutations in GA biosynthesis, metabolism, and signaling cascades influence plant height. Moreover, GA interacts with other phytohormones in the modulation of plant height. Here, we first briefly describe the regulation of plant height by altered GA pathway. Then, we depict effects of the crosstalk between GA and other phytohormones on plant height. We also dissect the co-localization of GA pathway genes and established quantitative genetic loci for plant height. Finally, we suggest ways forward for the application of hormone GA knowledge in breeding of crops with plant height ideotypes.

Aquatic plant control research

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pryfogle, P.A.; Rinehart, B.N.; Ghio, E.G.

The Northwest region of the United States contains extensive canal systems that transport water for hydropower generation. Nuisance plants, including algae, that grow in these systems reduce their hydraulic capacity through water displacement and increased surface friction. Most control methods are applied in an ad hoc fashion. The goal of this work is to develop cost-effective, environmentally sound, long-term management strategies to prevent and control nuisance algal growth. This paper reports on a multi-year study, performed in collaboration with the Pacific Gas & Electric Company, to investigate algal growth in their canal systems, and to evaluate various control methodologies. Threemore » types of controls, including mechanical, biological and chemical treatment, were selected for testing and evaluation. As part of this study, water quality data were collected and algal communities were sampled from numerous stations throughout the distribution system at regular intervals. This study resulted in a more comprehensive understanding of conditions leading to the development of nuisance algal growth, a better informed selection of treatment plans, and improved evaluation of the effectiveness for the control strategies selected for testing.« less

A coordinated MIMO control design for a power plant using improved sliding mode controller.

PubMed

Ataei, Mohammad; Hooshmand, Rahmat-Allah; Samani, Siavash Golmohammadi

2014-03-01

For the participation of the steam power plants in regulating the network frequency, boilers and turbines should be co-ordinately controlled in addition to the base load productions. Lack of coordinated control over boiler-turbine may lead to instability; oscillation in producing power and boiler parameters; reduction in the reliability of the unit; and inflicting thermodynamic tension on devices. This paper proposes a boiler-turbine coordinated multivariable control system based on improved sliding mode controller (ISMC). The system controls two main boiler-turbine parameters i.e., the turbine revolution and superheated steam pressure of the boiler output. For this purpose, a comprehensive model of the system including complete and exact description of the subsystems is extracted. The parameters of this model are determined according to our case study that is the 320MW unit of Islam-Abad power plant in Isfahan/Iran. The ISMC method is simulated on the power plant and its performance is compared with the related real PI (proportional-integral) controllers which have been used in this unit. The simulation results show the capability of the proposed controller system in controlling local network frequency and superheated steam pressure in the presence of load variations and disturbances of boiler. © 2013 ISA. Published by Elsevier Ltd. All rights reserved.

Fault-tolerant control of large space structures using the stable factorization approach

NASA Technical Reports Server (NTRS)

Razavi, H. C.; Mehra, R. K.; Vidyasagar, M.

1986-01-01

Large space structures are characterized by the following features: they are in general infinite-dimensional systems, and have large numbers of undamped or lightly damped poles. Any attempt to apply linear control theory to large space structures must therefore take into account these features. Phase I consisted of an attempt to apply the recently developed Stable Factorization (SF) design philosophy to problems of large space structures, with particular attention to the aspects of robustness and fault tolerance. The final report on the Phase I effort consists of four sections, each devoted to one task. The first three sections report theoretical results, while the last consists of a design example. Significant results were obtained in all four tasks of the project. More specifically, an innovative approach to order reduction was obtained, stabilizing controller structures for plants with an infinite number of unstable poles were determined under some conditions, conditions for simultaneous stabilizability of an infinite number of plants were explored, and a fault tolerance controller design that stabilizes a flexible structure model was obtained which is robust against one failure condition.

Interplay of HD-Zip II and III transcription factors in auxin-regulated plant development.

PubMed

Turchi, L; Baima, S; Morelli, G; Ruberti, I

2015-08-01

The homeodomain-leucine zipper (HD-Zip) class of transcription factors is unique to plants. HD-Zip proteins bind to DNA exclusively as dimers recognizing dyad symmetric sequences and act as positive or negative regulators of gene expression. On the basis of sequence homology in the HD-Zip DNA-binding domain, HD-Zip proteins have been grouped into four families (HD-Zip I-IV). Each HD-Zip family can be further divided into subfamilies containing paralogous genes that have arisen through genome duplication. Remarkably, all the members of the HD-Zip IIγ and -δ clades are regulated by light quality changes that induce in the majority of the angiosperms the shade-avoidance response, a process regulated at multiple levels by auxin. Intriguingly, it has recently emerged that, apart from their function in shade avoidance, the HD-Zip IIγ and -δ transcription factors control several auxin-regulated developmental processes, including apical embryo patterning, lateral organ polarity, and gynoecium development, in a white-light environment. This review presents recent advances in our understanding of HD-Zip II protein function in plant development, with particular emphasis on the impact of loss-of-function HD-Zip II mutations on auxin distribution and response. The review also describes evidence demonstrating that HD-Zip IIγ and -δ genes are directly and positively regulated by HD-Zip III transcription factors, primary determinants of apical shoot development, known to control the expression of several auxin biosynthesis, transport, and response genes. Finally, the interplay between HD-Zip II and III transcription factors in embryo apical patterning and organ polarity is discussed. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Controlled Ecological Life Support System: Use of Higher Plants

NASA Technical Reports Server (NTRS)

Tibbits, T. W.; Alford, D. K.

1982-01-01

Results of two workshops concerning the use of higher plants in Controlled Ecological Life Support Systems (CELSS) are summarized. Criteria for plant selection were identified from these categories: food production, nutrition, oxygen production and carbon dioxide utilization, water recycling, waste recycling, and other morphological and physiological considerations. Types of plant species suitable for use in CELSS, growing procedures, and research priorities were recommended. Also included are productivity values for selected plant species.

Expert System Control of Plant Growth in an Enclosed Space

NASA Technical Reports Server (NTRS)

May, George; Lanoue, Mark; Bathel, Matthew; Ryan, Robert E.

2008-01-01

The Expert System is an enclosed, controlled environment for growing plants, which incorporates a computerized, knowledge-based software program that is designed to capture the knowledge, experience, and problem-solving skills of one or more human experts in a particular discipline. The Expert System is trained to analyze crop/plant status, to monitor the condition of the plants and the environment, and to adjust operational parameters to optimize the plant-growth process. This system is intended to provide a way to remotely control plant growth with little or no human intervention. More specifically, the term control implies an autonomous method for detecting plant states such as health (biomass) or stress and then for recommending and implementing cultivation and/or remediation to optimize plant growth and to minimize consumption of energy and nutrients. Because of difficulties associated with delivering energy and nutrients remotely, a key feature of this Expert System is its ability to minimize this effort and to achieve optimum growth while taking into account the diverse range of environmental considerations that exist in an enclosed environment. The plant-growth environment for the Expert System could be made from a variety of structures, including a greenhouse, an underground cavern, or another enclosed chamber. Imaging equipment positioned within or around the chamber provides spatially distributed crop/plant-growth information. Sensors mounted in the chamber provide data and information pertaining to environmental conditions that could affect plant development. Lamps in the growth environment structure supply illumination, and other additional equipment in the chamber supplies essential nutrients and chemicals.

State of South Carolina Cooperative Aquatic Plant Control Program.

DTIC Science & Technology

1980-11-01

Carolina Water Resources Commission. Abstract: The proposed program provides for a comprehensive plan to control noxious aquatic plants within the state...public water bodies in South Carolina to a more natural condition by controlling the excessive growth of aquatic vegetation in the interest of...Herbicides Proposed for Use in the Aquatic Plant Management Program D Cost Estimate E EPA Established Tolerances for Selected Herbicides in Potable Water F

Health-aware Model Predictive Control of Pasteurization Plant

NASA Astrophysics Data System (ADS)

Karimi Pour, Fatemeh; Puig, Vicenç; Ocampo-Martinez, Carlos

2017-01-01

In order to optimize the trade-off between components life and energy consumption, the integration of a system health management and control modules is required. This paper proposes the integration of model predictive control (MPC) with a fatigue estimation approach that minimizes the damage of the components of a pasteurization plant. The fatigue estimation is assessed with the rainflow counting algorithm. Using data from this algorithm, a simplified model that characterizes the health of the system is developed and integrated with MPC. The MPC controller objective is modified by adding an extra criterion that takes into account the accumulated damage. But, a steady-state offset is created by adding this extra criterion. Finally, by including an integral action in the MPC controller, the steady-state error for regulation purpose is eliminated. The proposed control scheme is validated in simulation using a simulator of a utility-scale pasteurization plant.

Would the control of invasive alien plants reduce malaria transmission? A review.

PubMed

Stone, Christopher M; Witt, Arne B R; Walsh, Guillermo Cabrera; Foster, Woodbridge A; Murphy, Sean T

2018-02-01

Vector control has been the most effective preventive measure against malaria and other vector-borne diseases. However, due to concerns such as insecticide resistance and budget shortfalls, an integrated control approach will be required to ensure sustainable, long-term effectiveness. An integrated management strategy should entail some aspects of environmental management, relying on coordination between various scientific disciplines. Here, we review one such environmental control tactic: invasive alien plant management. This covers salient plant-mosquito interactions for both terrestrial and aquatic invasive plants and how these affect a vector's ability to transmit malaria. Invasive plants tend to have longer flowering durations, more vigorous growth, and their spread can result in an increase in biomass, particularly in areas where previously little vegetation existed. Some invasive alien plants provide shelter or resting sites for adult mosquitoes and are also attractive nectar-producing hosts, enhancing their vectorial capacity. We conclude that these plants may increase malaria transmission rates in certain environments, though many questions still need to be answered, to determine how often this conclusion holds. However, in the case of aquatic invasive plants, available evidence suggests that the management of these plants would contribute to malaria control. We also examine and review the opportunities for large-scale invasive alien plant management, including options for biological control. Finally, we highlight the research priorities that must be addressed in order to ensure that integrated vector and invasive alien plant management operate in a synergistic fashion.

Modeling, simulation, and control of an extraterrestrial oxygen production plant

NASA Technical Reports Server (NTRS)

Schooley, L.; Cellier, F.; Zeigler, B.; Doser, A.; Farrenkopf, G.

1991-01-01

The immediate objective is the development of a new methodology for simulation of process plants used to produce oxygen and/or other useful materials from local planetary resources. Computer communication, artificial intelligence, smart sensors, and distributed control algorithms are being developed and implemented so that the simulation or an actual plant can be controlled from a remote location. The ultimate result of this research will provide the capability for teleoperation of such process plants which may be located on Mars, Luna, an asteroid, or other objects in space. A very useful near-term result will be the creation of an interactive design tool, which can be used to create and optimize the process/plant design and the control strategy. This will also provide a vivid, graphic demonstration mechanism to convey the results of other researchers to the sponsor.

Autonomous Control of Nuclear Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Basher, H.

2003-10-20

A nuclear reactor is a complex system that requires highly sophisticated controllers to ensure that desired performance and safety can be achieved and maintained during its operations. Higher-demanding operational requirements such as reliability, lower environmental impacts, and improved performance under adverse conditions in nuclear power plants, coupled with the complexity and uncertainty of the models, necessitate the use of an increased level of autonomy in the control methods. In the opinion of many researchers, the tasks involved during nuclear reactor design and operation (e.g., design optimization, transient diagnosis, and core reload optimization) involve important human cognition and decisions that maymore » be more easily achieved with intelligent methods such as expert systems, fuzzy logic, neural networks, and genetic algorithms. Many experts in the field of control systems share the idea that a higher degree of autonomy in control of complex systems such as nuclear plants is more easily achievable through the integration of conventional control systems and the intelligent components. Researchers have investigated the feasibility of the integration of fuzzy logic, neural networks, genetic algorithms, and expert systems with the conventional control methods to achieve higher degrees of autonomy in different aspects of reactor operations such as reactor startup, shutdown in emergency situations, fault detection and diagnosis, nuclear reactor alarm processing and diagnosis, and reactor load-following operations, to name a few. With the advancement of new technologies and computing power, it is feasible to automate most of the nuclear reactor control and operation, which will result in increased safety and economical benefits. This study surveys current status, practices, and recent advances made towards developing autonomous control systems for nuclear reactors.« less

Effects of NOx control and plume mixing on nighttime chemical processing of plumes from coal-fired power plants

NASA Astrophysics Data System (ADS)

Brown, Steven S.; Dubé, William P.; Karamchandani, Prakash; Yarwood, Greg; Peischl, Jeff; Ryerson, Thomas B.; Neuman, J. Andrew; Nowak, John B.; Holloway, John S.; Washenfelder, Rebecca A.; Brock, Charles A.; Frost, Gregory J.; Trainer, Michael; Parrish, David D.; Fehsenfeld, Frederick C.; Ravishankara, A. R.

2012-04-01

Coal-fired electric power plants produce a large fraction of total U.S. NOx emissions, but NOx from this sector has been declining in the last decade owing to installation of control technology. Nighttime aircraft intercepts of plumes from two different Texas power plants (Oklaunion near Wichita Falls and W. A. Parish near Houston) with different control technologies demonstrate the effect of these reductions on nighttime NOxoxidation rates. The analysis shows that the spatial extent of nighttime-emitted plumes to be quite limited and that mixing of highly concentrated plume NOx with ambient ozone is a determining factor for its nighttime oxidation. The plume from the uncontrolled plant had full titration of ozone through 74 km/2.4 h of downwind transport that suppressed nighttime oxidation of NO2 to higher oxides of nitrogen across the majority of the plume. The plume from the controlled plant did not have sufficient NOx to titrate background ozone, which led to rapid nighttime oxidation of NO2 during downwind transport. A plume model that includes horizontal mixing and nighttime chemistry reproduces the observed structures of the nitrogen species in the plumes from the two plants. The model shows that NOx controls not only reduce the emissions directly but also lead to an additional overnight NOx loss of 36-44% on average. The maximum reduction for 12 h of transport in darkness was 73%. The results imply that power plant NOxemissions controls may produce a larger than linear reduction in next-day, downwind ozone production following nighttime transport.

B-Plant Canyon Ventilation Control System Description

DOE Office of Scientific and Technical Information (OSTI.GOV)

MCDANIEL, K.S.

1999-08-31

Project W-059 installed a new B Plant Canyon Ventilation System. Monitoring and control of the system is implemented by the Canyon Ventilation Control System (CVCS). This document describes the CVCS system components which include a Programmable Logic Controller (PLC) coupled with an Operator Interface Unit (OIU) and application software. This document also includes an Alarm Index specifying the setpoints and technical basis for system analog and digital alarms.

Methods and systems for seed planting management and control

DOEpatents

Svoboda, John M.; Hess, J. Richard; Hoskinson, Reed L.; Harker, David J.

2002-01-01

A seed planting system providing optimal seed spacing in an agricultural field. The seed planting system includes a mobile seed planter having one or more planting shoes, or members being adapted for towing by a farm vehicle or being self-propelled. Sensors, disposed proximate to respective planting shoes, detect seed planting events and send corresponding signals to a computer. Contemporaneously, a geospatial locator acquires, and transmits to the computer, the geospatial location of each planted seed. The computer correlates the geospatial location data with the seed deposition data and generates a seed distribution profile indicating the location of each seed planted in a zone of interest to enable the control of speed spacing.

Development of Automatic Control of Bayer Plant Digestion

NASA Astrophysics Data System (ADS)

Riffaud, J. P.

Supervisory computer control has been achieved in Alcan's Bayer Plants at Arvida, Quebec, Canada. The purpose of the automatic control system is to stabilize and consequently increase, the alumina/caustic ratio within the digester train and in the blow-off liquor. Measurements of the electrical conductivity of the liquor are obtained from electrodeless conductivity meters. These signals, along with several others are scanned by the computer and converted to engineering units, using specific relationships which are updated periodically for calibration purposes. On regular time intervals, values of ratio are compared to target values and adjustments are made to the bauxite flow entering the digesters. Dead time compensation included in the control algorithm enables a faster rate for corrections. Modification of production rate is achieved through careful timing of various flow changes. Calibration of the conductivity meters is achieved by sampling at intervals the liquor flowing through them, and analysing it with a thermometric titrator. Calibration of the thermometric titrator is done at intervals with a standard solution. Calculations for both calibrations are performed by computer from data entered by the analyst. The computer was used for on-line data collection, modelling of the digester system, calculation of disturbances and simulation of control strategies before implementing the most successful strategy in the Plant. Control of ratio has been improved by the integrated system, resulting in increased Plant productivity.

TCP Transcription Factors at the Interface between Environmental Challenges and the Plant's Growth Responses.

PubMed

Danisman, Selahattin

2016-01-01

Plants are sessile and as such their reactions to environmental challenges differ from those of mobile organisms. Many adaptions involve growth responses and hence, growth regulation is one of the most crucial biological processes for plant survival and fitness. The plant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, PCF1 (TCP) transcription factor family is involved in plant development from cradle to grave, i.e., from seed germination throughout vegetative development until the formation of flowers and fruits. TCP transcription factors have an evolutionary conserved role as regulators in a variety of plant species, including orchids, tomatoes, peas, poplar, cotton, rice and the model plant Arabidopsis. Early TCP research focused on the regulatory functions of TCPs in the development of diverse organs via the cell cycle. Later research uncovered that TCP transcription factors are not static developmental regulators but crucial growth regulators that translate diverse endogenous and environmental signals into growth responses best fitted to ensure plant fitness and health. I will recapitulate the research on TCPs in this review focusing on two topics: the discovery of TCPs and the elucidation of their evolutionarily conserved roles across the plant kingdom, and the variety of signals, both endogenous (circadian clock, plant hormones) and environmental (pathogens, light, nutrients), TCPs respond to in the course of their developmental roles.

Global scale environmental control of plant photosynthetic capacity

DOE PAGES

Ali, Ashehad; Xu, Chonggang; Rogers, Alistair; ...

2015-12-01

Photosynthetic capacity, determined by light harvesting and carboxylation reactions, is a key plant trait that determines the rate of photosynthesis; however, in Earth System Models (ESMs) at a reference temperature, it is either a fixed value for a given plant functional type or derived from a linear function of leaf nitrogen content. In this study, we conducted a comprehensive analysis that considered correlations of environmental factors with photosynthetic capacity as determined by maximum carboxylation (V c,m) rate scaled to 25°C (i.e., V c,25; μmol CO 2·m –2·s –1) and maximum electron transport rate (Jmax) scaled to 25°C (i.e., J 25;more » μmol electron·m –2·s –1) at the global scale. Our results showed that the percentage of variation in observed Vc,25 and J25 explained jointly by the environmental factors (i.e., day length, radiation, temperature, and humidity) were 2–2.5 times and 6–9 times of that explained by area-based leaf nitrogen content, respectively. Environmental factors influenced photosynthetic capacity mainly through photosynthetic nitrogen use efficiency, rather than through leaf nitrogen content. The combination of leaf nitrogen content and environmental factors was able to explain ~56% and ~66% of the variation in V c,25 and J 25 at the global scale, respectively. As a result, our analyses suggest that model projections of plant photosynthetic capacity and hence land–atmosphere exchange under changing climatic conditions could be substantially improved if environmental factors are incorporated into algorithms used to parameterize photosynthetic capacity in ESMs.« less

CONTROLLING MULTIPLE EMISSIONS FROM COAL-FIRED POWER PLANTS

EPA Science Inventory

The paper presents and analyzes nine existing and novel control technologies designed to achieve multipollutant emissions reductions. It provides an evaluation of multipollutant emission control technologies that are potentially available for coal-fired power plants of 25 MW capa...

Plant parasite control and soil fauna diversity.

PubMed

Lavelle, Patrick; Blouin, Manuel; Boyer, Johnny; Cadet, Patrice; Laffray, Daniel; Pham-Thi, Anh-Thu; Reversat, Georges; Settle, William; Zuily, Yasmine

2004-07-01

The use of pesticides to control plant parasites and diseases has generated serious problems of public health and environmental quality, leading to the promotion of alternative Integrated Pest Management strategies that tend to rely more on natural processes and the active participation of farmers as observers and experimenters in their own fields. We present three case studies that point at different options provided by locally available populations of soil organisms, the maintenance of diverse populations of pests or increased resistance of plants to pest attacks by their interactions with earthworms and other useful soil organisms. These examples demonstrate the diversity of options offered by the non-planned agro-ecosystem diversity in pest control and the need to identify management options that maintain this biodiversity.

Medicinal plants used to control internal and external parasites in goats.

PubMed

Sanhokwe, Marcia; Mupangwa, Johnfisher; Masika, Patrick J; Maphosa, Viola; Muchenje, Voster

2016-04-29

The use of medicinal plants plays a major role in the primary health care of animals in South Africa. A survey was conducted to document medicinal plants used to control parasites in goats in Kwezi and Ntambethemba villages in the Eastern Cape Province, South Africa. Information from 50 farmers and 3 herbalists was obtained through the use of a structured questionnaire, and a snowball sampling technique was used to identify key informants. The obtained data were analysed using PROC FREQ of SAS (2003), and fidelity level values were determined to estimate the healing potential of the mentioned plants. The survey revealed nine plant species belonging to eight families that were used to control parasites in goats. Asphodelaceae (22.22%) was the most frequently used plant family. Leaves were the most used plant parts, constituting 60.38%. They were prepared either as infusions or decoctions of single plants or in mixtures. Aloe ferox, Acokanthera oppositifolia and Elephantorrhiza elephantina were the plants having the highest fidelity level for their use to control parasites, each scoring 100%, followed by Albuca setosa (83.33%). The study revealed low knowledge about ethnoveterinary medicine in the study area. It also revealed that information on ethno-veterinary medicine in this area is mostly confined to older people and there is danger that this knowledge can be lost before being passed on to other generations. Therefore, there is an urgent need to document information on these plant species so that the future generation can benefit. Further investigation should be carried out to validate the efficacy and safety of the above-mentioned plants so as to provide cheap alternative ways of controlling parasites.

Outside-in control -Does plant cell wall integrity regulate cell cycle progression?

PubMed

Gigli-Bisceglia, Nora; Hamann, Thorsten

2018-04-13

During recent years it has become accepted that plant cell walls are not inert objects surrounding all plant cells but are instead highly dynamic, plastic structures. They are involved in a large number of cell biological processes and contribute actively to plant growth, development and interaction with environment. Therefore, it is not surprising that cellular processes can control plant cell wall integrity while, simultaneously, cell wall integrity can influence cellular processes. In yeast and animal cells such a bi-directional relationship also exists between the yeast/animal extra-cellular matrices and the cell cycle. In yeast, the cell wall integrity maintenance mechanism and a dedicated plasmamembrane integrity checkpoint are mediating this relationship. Recent research has yielded insights into the mechanism controlling plant cell wall metabolism during cytokinesis. However, knowledge regarding putative regulatory pathways controlling adaptive modifications in plant cell cycle activity in response to changes in the state of the plant cell wall are not yet identified. In this review, we summarize similarities and differences in regulatory mechanisms coordinating extra cellular matrices and cell cycle activity in animal and yeast cells, discuss the available evidence supporting the existence of such a mechanism in plants and suggest that the plant cell wall integrity maintenance mechanism might also control cell cycle activity in plant cells. This article is protected by copyright. All rights reserved.

Agricultural Plant Pest Control. Manual 93.

ERIC Educational Resources Information Center

Missouri Univ., Columbia. Agricultural Experiment Station.

This training manual provides information needed to meet the minimum EPA standards for certification as a commercial applicator of pesticides for the agricultural plant pest control category. The text discusses the insect pests including caterpillars, beetles, and soil inhabiting insects; diseases and nematodes; and weeds. Consideration is given…

Plant-Derived Transcription Factors for Orthologous Regulation of Gene Expression in the Yeast Saccharomyces cerevisiae.

PubMed

Naseri, Gita; Balazadeh, Salma; Machens, Fabian; Kamranfar, Iman; Messerschmidt, Katrin; Mueller-Roeber, Bernd

2017-09-15

Control of gene expression by transcription factors (TFs) is central in many synthetic biology projects for which a tailored expression of one or multiple genes is often needed. As TFs from evolutionary distant organisms are unlikely to affect gene expression in a host of choice, they represent excellent candidates for establishing orthogonal control systems. To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a library of 106 different combinations of chromosomally integrated TFs, activation domains (yeast GAL4 AD, herpes simplex virus VP64, and plant EDLL) and synthetic promoters harboring cognate cis-regulatory motifs driving a yEGFP reporter. Transcriptional output of the different driver/reporter combinations varied over a wide spectrum, with EDLL being a considerably stronger transcription activation domain in yeast than the GAL4 activation domain, in particular when fused to Arabidopsis NAC TFs. Notably, the strength of several NAC-EDLL fusions exceeded that of the strong yeast TDH3 promoter by 6- to 10-fold. We furthermore show that plant TFs can be used to build regulatory systems encoded by centromeric or episomal plasmids. Our library of TF-DNA binding site combinations offers an excellent tool for diverse synthetic biology applications in yeast.

Continuing Weed Control Benefits Young Planted Black Walnut

Treesearch

John E. Krajicek; Robert D. Williams

1971-01-01

Cultivation, atrazine, and simazine were used for weed control 1, 2, and 3 years following planting of black walnut in Iowa and Indiana. In Iowa, 2 or more years of weed control resulted in the best seedling growth, but in Indiana 3 years proved best. Method of weed control had no significant effect on seedling growth in Iowa, but chemical control resulted in better...

The Organization of Controller Motifs Leading to Robust Plant Iron Homeostasis

PubMed Central

Agafonov, Oleg; Selstø, Christina Helen; Thorsen, Kristian; Xu, Xiang Ming; Drengstig, Tormod; Ruoff, Peter

2016-01-01

Iron is an essential element needed by all organisms for growth and development. Because iron becomes toxic at higher concentrations iron is under homeostatic control. Plants face also the problem that iron in the soil is tightly bound to oxygen and difficult to access. Plants have therefore developed special mechanisms for iron uptake and regulation. During the last years key components of plant iron regulation have been identified. How these components integrate and maintain robust iron homeostasis is presently not well understood. Here we use a computational approach to identify mechanisms for robust iron homeostasis in non-graminaceous plants. In comparison with experimental results certain control arrangements can be eliminated, among them that iron homeostasis is solely based on an iron-dependent degradation of the transporter IRT1. Recent IRT1 overexpression experiments suggested that IRT1-degradation is iron-independent. This suggestion appears to be misleading. We show that iron signaling pathways under IRT1 overexpression conditions become saturated, leading to a breakdown in iron regulation and to the observed iron-independent degradation of IRT1. A model, which complies with experimental data places the regulation of cytosolic iron at the transcript level of the transcription factor FIT. Including the experimental observation that FIT induces inhibition of IRT1 turnover we found a significant improvement in the system’s response time, suggesting a functional role for the FIT-mediated inhibition of IRT1 degradation. By combining iron uptake with storage and remobilization mechanisms a model is obtained which in a concerted manner integrates iron uptake, storage and remobilization. In agreement with experiments the model does not store iron during its high-affinity uptake. As an iron biofortification approach we discuss the possibility how iron can be accumulated even during high-affinity uptake. PMID:26800438

33 CFR Appendix A to Part 273 - Aquatic Plant Control Program Legislative Authority

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Aquatic Plant Control Program... OF THE ARMY, DEPARTMENT OF DEFENSE AQUATIC PLANT CONTROL Pt. 273, App. A Appendix A to Part 273—Aquatic Plant Control Program Legislative Authority Section 104 of the Rivers and Harbors Act, approved 3...

33 CFR Appendix A to Part 273 - Aquatic Plant Control Program Legislative Authority

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Aquatic Plant Control Program... OF THE ARMY, DEPARTMENT OF DEFENSE AQUATIC PLANT CONTROL Pt. 273, App. A Appendix A to Part 273—Aquatic Plant Control Program Legislative Authority Section 104 of the Rivers and Harbors Act, approved 3...

The Complexity of Vesicle Transport Factors in Plants Examined by Orthology Search

PubMed Central

Mirus, Oliver; Scharf, Klaus-Dieter; Fragkostefanakis, Sotirios; Schleiff, Enrico

2014-01-01

Vesicle transport is a central process to ensure protein and lipid distribution in eukaryotic cells. The current knowledge on the molecular components and mechanisms of this process is majorly based on studies in Saccharomyces cerevisiae and Arabidopsis thaliana, which revealed 240 different proteinaceous factors either experimentally proven or predicted to be involved in vesicle transport. In here, we performed an orthologue search using two different algorithms to identify the components of the secretory pathway in yeast and 14 plant genomes by using the ‘core-set’ of 240 factors as bait. We identified 4021 orthologues and (co-)orthologues in the discussed plant species accounting for components of COP-II, COP-I, Clathrin Coated Vesicles, Retromers and ESCRTs, Rab GTPases, Tethering factors and SNAREs. In plants, we observed a significantly higher number of (co-)orthologues than yeast, while only 8 tethering factors from yeast seem to be absent in the analyzed plant genomes. To link the identified (co-)orthologues to vesicle transport, the domain architecture of the proteins from yeast, genetic model plant A. thaliana and agriculturally relevant crop Solanum lycopersicum has been inspected. For the orthologous groups containing (co-)orthologues from yeast, A. thaliana and S. lycopersicum, we observed the same domain architecture for 79% (416/527) of the (co-)orthologues, which documents a very high conservation of this process. Further, publically available tissue-specific expression profiles for a subset of (co-)orthologues found in A. thaliana and S. lycopersicum suggest that some (co-)orthologues are involved in tissue-specific functions. Inspection of localization of the (co-)orthologues based on available proteome data or localization predictions lead to the assignment of plastid- as well as mitochondrial localized (co-)orthologues of vesicle transport factors and the relevance of this is discussed. PMID:24844592

Controlled ecological life support system higher plant flight experiments

NASA Technical Reports Server (NTRS)

Tibbitts, T. W.; Wheeler, R. M.

1984-01-01

Requirements for spaceflight experments which involve higher plants were determined. The plants are studied for use in controlled ecological life support systems (CELSS). Two categories of research requirements are discussed: (1) the physical needs which include nutrient, water and gas exchange requirements; (2) the biological and physiological functions which affect plants in zero gravity environments. Physical problems studies are given the priority since they affect all biological experiments.

In silico mining and PCR-based approaches to transcription factor discovery in non-model plants: gene discovery of the WRKY transcription factors in conifers.

PubMed

Liu, Jun-Jun; Xiang, Yu

2011-01-01

WRKY transcription factors are key regulators of numerous biological processes in plant growth and development, as well as plant responses to abiotic and biotic stresses. Research on biological functions of plant WRKY genes has focused in the past on model plant species or species with largely characterized transcriptomes. However, a variety of non-model plants, such as forest conifers, are essential as feed, biofuel, and wood or for sustainable ecosystems. Identification of WRKY genes in these non-model plants is equally important for understanding the evolutionary and function-adaptive processes of this transcription factor family. Because of limited genomic information, the rarity of regulatory gene mRNAs in transcriptomes, and the sequence divergence to model organism genes, identification of transcription factors in non-model plants using methods similar to those generally used for model plants is difficult. This chapter describes a gene family discovery strategy for identification of WRKY transcription factors in conifers by a combination of in silico-based prediction and PCR-based experimental approaches. Compared to traditional cDNA library screening or EST sequencing at transcriptome scales, this integrated gene discovery strategy provides fast, simple, reliable, and specific methods to unveil the WRKY gene family at both genome and transcriptome levels in non-model plants.

Designing propagation environments in forest and native plant nurseries

Treesearch

Thomas D. Landis

2013-01-01

Propagation environments are areas that have been modified for plant growth, and can be designed using the law of limiting factors. Identifying critical factors that are most limiting to optimal plant growth is helpful when developing both bareroot and container nurseries. Propagation environments can be categorized into minimally-controlled, semi-controlled, and fully...

A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells.

PubMed

Chatelle, Claire; Ochoa-Fernandez, Rocio; Engesser, Raphael; Schneider, Nils; Beyer, Hannes M; Jones, Alex R; Timmer, Jens; Zurbriggen, Matias D; Weber, Wilfried

2018-05-18

The ever-increasing complexity of synthetic gene networks and applications of synthetic biology requires precise and orthogonal gene expression systems. Of particular interest are systems responsive to light as they enable the control of gene expression dynamics with unprecedented resolution in space and time. While broadly used in mammalian backgrounds, however, optogenetic approaches in plant cells are still limited due to interference of the activating light with endogenous photoreceptors. Here, we describe the development of the first synthetic light-responsive system for the targeted control of gene expression in mammalian and plant cells that responds to the green range of the light spectrum in which plant photoreceptors have minimal activity. We first engineered a system based on the light-sensitive bacterial transcription factor CarH and its cognate DNA operator sequence CarO from Thermus thermophilus to control gene expression in mammalian cells. The system was functional in various mammalian cell lines, showing high induction (up to 350-fold) along with low leakiness, as well as high reversibility. We quantitatively described the systems characteristics by the development and experimental validation of a mathematical model. Finally, we transferred the system into A. thaliana protoplasts and demonstrated gene repression in response to green light. We expect that this system will provide new opportunities in applications based on synthetic gene networks and will open up perspectives for optogenetic studies in mammalian and plant cells.

50. EASTSIDE PLANT: GENERAL VIEW OF GENERATOR EXCITER AND CONTROL ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

50. EASTSIDE PLANT: GENERAL VIEW OF GENERATOR EXCITER AND CONTROL MECHANISM - American Falls Water, Power & Light Company, Island Power Plant, Snake River, below American Falls Dam, American Falls, Power County, ID

Light harvesting control in plants.

PubMed

Ruban, Alexander V

2018-05-23

In 1991, my colleagues and I published a hypothesis article that proposed a mechanism that controls light harvesting in plants and protects them against photodamage. The major light harvesting complex, LHCII, was suggested to undergo aggregation upon exposure of the plant to damaging levels of light. Aggregated LHCII was found to be much less efficient in light harvesting, as it promptly dissipated absorbed energy into heat, possessing a very low chlorophyll fluorescence yield. Non-photochemical quenching (NPQ) is a term coined to describe this reduction in chlorophyll fluorescence yield. This article is a story of how the hypothesis that LHCII aggregation is involved in NPQ is developed into a model that is now becoming broadly accepted by the research community. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Redox modulation of plant developmental regulators from the class I TCP transcription factor family.

PubMed

Viola, Ivana L; Güttlein, Leandro N; Gonzalez, Daniel H

2013-07-01

TEOSINTE BRANCHED1-CYCLOIDEA-PROLIFERATING CELL FACTOR1 (TCP) transcription factors participate in plant developmental processes associated with cell proliferation and growth. Most members of class I, one of the two classes that compose the family, have a conserved cysteine at position 20 (Cys-20) of the TCP DNA-binding and dimerization domain. We show that Arabidopsis (Arabidopsis thaliana) class I proteins with Cys-20 are sensitive to redox conditions, since their DNA-binding activity is inhibited after incubation with the oxidants diamide, oxidized glutathione, or hydrogen peroxide or with nitric oxide-producing agents. Inhibition can be reversed by treatment with the reductants dithiothreitol or reduced glutathione or by incubation with the thioredoxin/thioredoxin reductase system. Mutation of Cys-20 in the class I protein TCP15 abolished its redox sensitivity. Under oxidizing conditions, covalently linked dimers were formed, suggesting that inactivation is associated with the formation of intermolecular disulfide bonds. Inhibition of class I TCP protein activity was also observed in vivo, in yeast (Saccharomyces cerevisiae) cells expressing TCP proteins and in plants after treatment with redox agents. This inhibition was correlated with modifications in the expression of the downstream CUC1 gene in plants. Modeling studies indicated that Cys-20 is located at the dimer interface near the DNA-binding surface. This places this residue in the correct orientation for intermolecular disulfide bond formation and explains the sensitivity of DNA binding to the oxidation of Cys-20. The redox properties of Cys-20 and the observed effects of cellular redox agents both in vitro and in vivo suggest that class I TCP protein action is under redox control in plants.

Fusaric acid is a crucial factor in the disturbance of leaf water imbalance in Fusarium-infected banana plants.

PubMed

Dong, Xian; Ling, Ning; Wang, Min; Shen, Qirong; Guo, Shiwei

2012-11-01

Fusarium wilt of banana is caused by Fusarium oxysporum f. sp. cubense infection. The initial chlorosis symptoms occur progressively from lower to upper leaves, with wilt symptoms subsequently occurring in the whole plant. To determine the effect of the pathogen infection on the gas exchange characteristics and water content in banana leaves, hydroponic experiments with pathogen inoculation were conducted in a greenhouse. Compared with control plants, infected banana seedlings showed a higher leaf temperature as determined by thermal imaging. Reduced stomatal conductance (g(s)) and transpiration rate (E) in infected plants resulted in lower levels of water loss than in control plants. Water potential in heavily diseased plants (II) was significantly reduced and the E/g(s) ratio was higher than in noninfected plants, indicating the occurrence of uncontrolled water loss not regulated by stomata in diseased plants. As no pathogen colonies were detected from the infected plant leaves, the crude toxin was extracted from the pathogen culture and evaluated about the effect on banana plant to further investigate the probable reason of these physiological changes in Fusarium-infected banana leaf. The phytotoxin fusaric acid (FA) was found in the crude toxin, and both crude toxin and pure FA had similar effects as the pathogen infection on the physiological changes in banana leaf. Additionally, FA was present at all positions in diseased plants and its concentration was positively correlated with the incidence of disease symptoms. Taken together, these observations indicated that FA secreted by the pathogen is an important factor involved in the disturbance of leaf temperature, resulting in uncontrolled leaf water loss and electrolyte leakage due to damaging the cell membrane. In conclusion, FA plays a critical role in accelerating the development of Fusarium wilt in banana plants by acting as a phytotoxin. Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Revisiting the Role of Plant Transcription Factors in the Battle against Abiotic Stress.

PubMed

Khan, Sardar-Ali; Li, Meng-Zhan; Wang, Suo-Min; Yin, Hong-Ju

2018-05-31

Owing to diverse abiotic stresses and global climate deterioration, the agricultural production worldwide is suffering serious losses. Breeding stress-resilient crops with higher quality and yield against multiple environmental stresses via application of transgenic technologies is currently the most promising approach. Deciphering molecular principles and mining stress-associate genes that govern plant responses against abiotic stresses is one of the prerequisites to develop stress-resistant crop varieties. As molecular switches in controlling stress-responsive genes expression, transcription factors (TFs) play crucial roles in regulating various abiotic stress responses. Hence, functional analysis of TFs and their interaction partners during abiotic stresses is crucial to perceive their role in diverse signaling cascades that many researchers have continued to undertake. Here, we review current developments in understanding TFs, with particular emphasis on their functions in orchestrating plant abiotic stress responses. Further, we discuss novel molecular mechanisms of their action under abiotic stress conditions. This will provide valuable information for understanding regulatory mechanisms to engineer stress-tolerant crops.

Factors controlling peat chemistry and vegetation composition in Sudbury peatlands after 30 years of pollution emission reductions.

PubMed

Barrett, Sophie E; Watmough, Shaun A

2015-11-01

The objective of this research was to assess factors controlling peat and plant chemistry, and vegetation composition in 18 peatlands surrounding Sudbury after more than 30 years of large (>95%) pollution emission reductions. Sites closer to the main Copper Cliff smelter had more humified peat and the surface horizons were greatly enriched in copper (Cu) and nickel (Ni). Copper and Ni concentrations in peat were significantly correlated with that in the plant tissue of Chamaedaphne calyculata. The pH of peat was the strongest determining factor for species richness, diversity, and community composition, although percent vascular plant cover was strongly negatively correlated with surface Cu and Ni concentrations in peat. Sphagnum frequency was also negatively related to peat Cu and Ni concentrations indicating sites close to Copper Cliff smelter remain adversely impacted by industrial activities. Copyright © 2015 Elsevier Ltd. All rights reserved.

A discrete control model of PLANT

NASA Technical Reports Server (NTRS)

Mitchell, C. M.

1985-01-01

A model of the PLANT system using the discrete control modeling techniques developed by Miller is described. Discrete control models attempt to represent in a mathematical form how a human operator might decompose a complex system into simpler parts and how the control actions and system configuration are coordinated so that acceptable overall system performance is achieved. Basic questions include knowledge representation, information flow, and decision making in complex systems. The structure of the model is a general hierarchical/heterarchical scheme which structurally accounts for coordination and dynamic focus of attention. Mathematically, the discrete control model is defined in terms of a network of finite state systems. Specifically, the discrete control model accounts for how specific control actions are selected from information about the controlled system, the environment, and the context of the situation. The objective is to provide a plausible and empirically testable accounting and, if possible, explanation of control behavior.

Spectral composition of light and growing of plants in controlled environments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tikhomirov, A.A.

1994-12-31

The curve of the action spectrum of photosynthesis is examined under the controlled influence of light that involves av 3-5 minutes irradiation with one specific spectral flux. Different curves were obtained for spectral affectivity of green leaf photosynthesis when plants have had long duration adaptation to lamps of different spectral composition and PAR intensity. The author suggests that the illumination of plants in natural conditions does not have to be copied for growing plants in controlled environments.

[Cytological study of radiation induced alterations in cytoplasmic factors controlling male sterility]. Progress report, 1971--1973

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1973-01-01

Progress is reported on studies of cytoplasmic factors controlling male sterility in plants. Results are reported from cytological comparisons of fertile selections from gamma -irradiated corn with male steriles, mainliners, and restored steriles, in which no consistent differences in cytoplasmic constituents were observed. Results of cytological and genetic studies on mutants of Neurospora crassa, petunia, tobacco, sorghum, sugar beets, Vicia faba, and several gymnosperms are summarized. The relationship between male, sterility of plants and their susceptibility to virus and fungus infections was also studied. (CH)

[The epidemiological characteristics and correlated factors of daily hassles for thermal power plant workers].

PubMed

Wu, Hui; Yu, Shan-fa; Zhou, Wen-hui; Gu, Gui-zhen

2012-07-01

This study aimed to investigate the epidemiological characteristics and correlated factors of daily hassles among thermal power plant workers. A mass screening of daily hassles and correlated factors was conducted on 498 workers from a thermal power plant in Zhengzhou in July, 2008. The questionnaires included Daily Hassles Questionnaires, Work Roles Questionnaires, Job Content Questionnaires (Chinese version), Effort-Reward Imbalance (Chinese version), Work Locus of Control Scale and Type A Behavior Scale, with content covering demographic characters and occupational stress correlated factors among subjects. The daily hassles was divided into lower level and higher level according to scores, and the epidemiological characteristics and correlated factors of daily hassles were analyzed. A total of 446 qualified questionnaires were obtained, effective response rate was 89.6% (446/498). For respondents, the age was (36.96 ± 6.49) years old, working length of the current job was (12.05 ± 7.54) years, the daily hassles scores was (9.01 ± 2.50), and the prevalence rate of the higher level of daily hassles was 34.1% (152/446). The multiple non-conditional logistic regression analysis showed 5-14 years' working length of current job (OR = 0.451, 95%CI: 0.225 - 0.904), average income > 3000 yuan(OR = 0.372, 95%CI: 0.202 - 0.684), reward (OR = 0.557, 95%CI: 0.325 - 0.954) and coping strategy (OR = 0.552, 95%CI: 0.330 - 0.925) were negatively correlated with daily hassles, and shift-work (OR = 1.887, 95%CI: 1.108 - 3.215), effort (OR = 2.053, 95%CI: 1.198 - 3.519), psychological demand (OR = 1.797, 95%CI: 1.049 - 3.078), negative affectivity (OR = 3.421, 95%CI: 2.065 - 5.668) were positively correlated with daily hassles. The prevalence rate of the higher level of daily hassles was considerable high for thermal power plant workers. Its negative correlated factors included 5 - 14 years' working length of the current job, average income > 3000 yuan, reward and coping

A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota

PubMed Central

Lapidot, Moshe; Karniel, Uri; Gelbart, Dana; Fogel, Doron; Evenor, Dalia; Kutsher, Yaarit; Makhbash, Zion; Nahon, Sahadia; Shlomo, Haviva; Chen, Lea; Reuveni, Moshe; Levin, Ilan

2015-01-01

Tomato yellow leaf curl virus (TYLCV) is a devastating disease of tomato (Solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. The TYLCV-resistant line TY172 carries a major recessive locus for TYLCV resistance, designated ty-5, on chromosome 4. In this study, the association between 27 polymorphic DNA markers, spanning the ty-5 locus, and the resistance characteristics of individual plants inoculated with TYLCV in 51 segregating recombinant populations were analyzed. These analyses localized ty-5 into a 425 bp region containing two transversions: one in the first exon of a gene encoding the tomato homolog of the messenger RNA surveillance factor Pelota (Pelo), and a second in its proximal promoter. Analyses of susceptible and resistant lines revealed that the relative transcript level of the gene remained unchanged, regardless of whether the plants were infected with TYLCV or not. This suggests that the polymorphism discovered in the coding region of the gene controls the resistance. Silencing of Pelo in a susceptible line rendered the transgenic plants highly resistant, while in the resistant line TY172 had no effect on symptom development. In addition, over-expression of the susceptible allele of the gene in the resistant TY172 line rendered it susceptible, while over-expression of the resistant allele in susceptible plants had no effect. These results confirm that Pelo is the gene controlling resistance at the ty-5 locus. Pelo, implicated in the ribosome recycling-phase of protein synthesis, offers an alternative route to promote resistance to TYLCV and other viruses. PMID:26448569

Molecular mechanisms of nematode-nematophagous microbe interactions: basis for biological control of plant-parasitic nematodes.

PubMed

Li, Juan; Zou, Chenggang; Xu, Jianping; Ji, Xinglai; Niu, Xuemei; Yang, Jinkui; Huang, Xiaowei; Zhang, Ke-Qin

2015-01-01

Plant-parasitic nematodes cause significant damage to a broad range of vegetables and agricultural crops throughout the world. As the natural enemies of nematodes, nematophagous microorganisms offer a promising approach to control the nematode pests. Some of these microorganisms produce traps to capture and kill the worms from the outside. Others act as internal parasites to produce toxins and virulence factors to kill the nematodes from within. Understanding the molecular basis of microbe-nematode interactions provides crucial insights for developing effective biological control agents against plant-parasitic nematodes. Here, we review recent advances in our understanding of the interactions between nematodes and nematophagous microorganisms, with a focus on the molecular mechanisms by which nematophagous microorganisms infect nematodes and on the nematode defense against pathogenic attacks. We conclude by discussing several key areas for future research and development, including potential approaches to apply our recent understandings to develop effective biocontrol strategies.

Advanced Outage and Control Center: Strategies for Nuclear Plant Outage Work Status Capabilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gregory Weatherby

The research effort is a part of the Light Water Reactor Sustainability (LWRS) Program. LWRS is a research and development program sponsored by the Department of Energy, performed in close collaboration with industry to provide the technical foundations for licensing and managing the long-term, safe and economical operation of current nuclear power plants. The LWRS Program serves to help the US nuclear industry adopt new technologies and engineering solutions that facilitate the continued safe operation of the plants and extension of the current operating licenses. The Outage Control Center (OCC) Pilot Project was directed at carrying out the applied researchmore » for development and pilot of technology designed to enhance safe outage and maintenance operations, improve human performance and reliability, increase overall operational efficiency, and improve plant status control. Plant outage management is a high priority concern for the nuclear industry from cost and safety perspectives. Unfortunately, many of the underlying technologies supporting outage control are the same as those used in the 1980’s. They depend heavily upon large teams of staff, multiple work and coordination locations, and manual administrative actions that require large amounts of paper. Previous work in human reliability analysis suggests that many repetitive tasks, including paper work tasks, may have a failure rate of 1.0E-3 or higher (Gertman, 1996). With between 10,000 and 45,000 subtasks being performed during an outage (Gomes, 1996), the opportunity for human error of some consequence is a realistic concern. Although a number of factors exist that can make these errors recoverable, reducing and effectively coordinating the sheer number of tasks to be performed, particularly those that are error prone, has the potential to enhance outage efficiency and safety. Additionally, outage management requires precise coordination of work groups that do not always share similar objectives

Aquatic Plant Control Research Program. Large-Scale Operations Management Test of Use of the White Amur for Control of Problem Aquatic Plants. Report 5. Synthesis Report.

DTIC Science & Technology

1984-06-01

RD-Rl45 988 AQUATIC PLANT CONTROL RESEARCH PROGRAM LARGE-SCALE 1/2 OPERATIONS MANAGEMENT ..(U) ARMY ENGINEER WATERWAYS EXPERIMENT STATION VICKSBURG MS...REPORT A-78-2 LARGE-SCALE OPERATIONS MANAGEMENT TEST OF USE OF THE WHITE AMUR FOR -, CONTROL OF PROBLEM AQUATIC PLANTS Report 5 SYNTHESIS REPORT bv Andrew...Corps of Engineers Washington, DC 20314 84 0,_1 oil.. LARGE-SCALE OPERATIONS MANAGEMENT TEST OF USE OF THE WHITE AMUR FOR CONTROL OF PROBLEM AQUATIC

Plant chromatin warms up in Madrid

PubMed Central

Jarillo, José A; Gaudin, Valerie; Hennig, Lars; Köhler, Claudia; Piñeiro, Manuel

2014-01-01

The 3rd European Workshop on Plant Chromatin (EWPC) was held on August 2013 in Madrid, Spain. A number of different topics on plant chromatin were presented during the meeting, including new factors mediating Polycomb Group protein function in plants, chromatin-mediated reprogramming in plant developmental transitions, the role of histone variants, and newly identified chromatin remodeling factors. The function of interactions between chromatin and transcription factors in the modulation of gene expression, the role of chromatin dynamics in the control of nuclear processes and the influence of environmental factors on chromatin organization were also reported. In this report, we highlight some of the new insights emerging in this growing area of research, presented at the 3rd EWPC. PMID:24504145

Leaf shape: genetic controls and environmental factors.

PubMed

Tsukaya, Hirokazu

2005-01-01

In recent years, many genes have been identified that are involved in the developmental processes of leaf morphogenesis. Here, I review the mechanisms of leaf shape control in a model plant, Arabidopsis thaliana, focusing on genes that fulfill special roles in leaf development. The lateral, two-dimensional expansion of leaf blades is highly dependent on the determination of the dorsoventrality of the primordia, a defining characteristic of leaves. Having a determinate fate is also a characteristic feature of leaves and is controlled by many factors. Lateral expansion is not only controlled by general regulators of cell cycling, but also by the multi-level regulation of meristematic activities, e.g., specific control of cell proliferation in the leaf-length direction, in leaf margins and in parenchymatous cells. In collaboration with the polarized control of leaf cell elongation, these redundant and specialized regulating systems for cell cycling in leaf lamina may realize the elegantly smooth, flat structure of leaves. The unified, flat shape of leaves is also dependent on the fine integration of cell proliferation and cell enlargement. Interestingly, while a decrease in the number of cells in leaf primordia can trigger a cell volume increase, an increase in the number of cells does not trigger a cell volume decrease. This phenomenon is termed compensation and suggests the existence of some systems for integration between cell cycling and cell enlargement in leaf primordia via cell-cell communication. The environmental adjustment of leaf expansion to light conditions and gravity is also summarized.

Factors associated with plant species richness in a coastal tall-grass prairie

USGS Publications Warehouse

Grace, James B.; Allain, Larry K.; Allen, Charles

2000-01-01

In this study we examine the factors associated with variations in species richness within a remnant tall-grass prairie in order to gain insight into the relative importance of controlling variables. The study area was a small, isolated prairie surrounded by wetlands and located within the coastal prairie region, which occurs along the northwestern Gulf of Mexico coastal plain. Samples were taken along three transects that spanned the prairie. Parameters measured included micro-elevation, soil characteristics, indications of recent disturbance, above-ground biomass (including litter), light penetration through the plant canopy, and species richness. Species richness was found to correlate with micro-elevation, certain soil parameters, and light penetration through the canopy, but not with above-ground biomass. Structural equation analysis was used to assess the direct and indirect effects of micro-elevation, soil properties, disturbance, and indicators of plant abundance on species richness. The results of this analysis showed that observed variations in species richness were primarily associated with variations in environmental effects (from soil and microtopography) and were largely unrelated to variations in measures of plant abundance (biomass and light penetration). These findings suggest that observed variations in species richness in this system primarily resulted from environmental effects on the species pool. These results fit with a growing body of information that suggests that environmental effects on species richness are of widespread importance.

HFE safety reviews of advanced nuclear power plant control rooms

NASA Technical Reports Server (NTRS)

Ohara, John

1994-01-01

Advanced control rooms (ACR's) will utilize human-system interface (HSI) technologies that may have significant implications for plant safety in that they will affect the operator's overall role and means of interacting with the system. The Nuclear Regulatory Commission (NRC) reviews the human factors engineering (HFE) aspects of HSI's to ensure that they are designed to good HFE principles and support performance and reliability in order to protect public health and safety. However, the only available NRC guidance was developed more than ten years ago, and does not adequately address the human performance issues and technology changes associated with ACR's. Accordingly, a new approach to ACR safety reviews was developed based upon the concept of 'convergent validity'. This approach to ACR safety reviews is described.

Hormonal control of cold stress responses in plants.

PubMed

Eremina, Marina; Rozhon, Wilfried; Poppenberger, Brigitte

2016-02-01

Cold stress responses in plants are highly sophisticated events that alter the biochemical composition of cells for protection from damage caused by low temperatures. In addition, cold stress has a profound impact on plant morphologies, causing growth repression and reduced yields. Complex signalling cascades are utilised to induce changes in cold-responsive gene expression that enable plants to withstand chilling or even freezing temperatures. These cascades are governed by the activity of plant hormones, and recent research has provided a better understanding of how cold stress responses are integrated with developmental pathways that modulate growth and initiate other events that increase cold tolerance. Information on the hormonal control of cold stress signalling is summarised to highlight the significant progress that has been made and indicate gaps that still exist in our understanding.

33 CFR Appendix A to Part 273 - Aquatic Plant Control Program Legislative Authority

Code of Federal Regulations, 2014 CFR

2014-07-01

..., and other obnoxious aquatic plant growths, from the navigable waters, tributary streams, connecting... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Aquatic Plant Control Program... OF THE ARMY, DEPARTMENT OF DEFENSE AQUATIC PLANT CONTROL Pt. 273, App. A Appendix A to Part 273...

33 CFR Appendix A to Part 273 - Aquatic Plant Control Program Legislative Authority

Code of Federal Regulations, 2013 CFR

2013-07-01

..., and other obnoxious aquatic plant growths, from the navigable waters, tributary streams, connecting... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Aquatic Plant Control Program... OF THE ARMY, DEPARTMENT OF DEFENSE AQUATIC PLANT CONTROL Pt. 273, App. A Appendix A to Part 273...

33 CFR Appendix A to Part 273 - Aquatic Plant Control Program Legislative Authority

Code of Federal Regulations, 2012 CFR

2012-07-01

..., and other obnoxious aquatic plant growths, from the navigable waters, tributary streams, connecting... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Aquatic Plant Control Program... OF THE ARMY, DEPARTMENT OF DEFENSE AQUATIC PLANT CONTROL Pt. 273, App. A Appendix A to Part 273...

Control system development for a 1 MW/e/ solar thermal power plant

NASA Technical Reports Server (NTRS)

Daubert, E. R.; Bergthold, F. M., Jr.; Fulton, D. G.

1981-01-01

The point-focusing distributed receiver power plant considered consists of a number of power modules delivering power to a central collection point. Each power module contains a parabolic dish concentrator with a closed-cycle receiver/turbine/alternator assembly. Currently, a single-module prototype plant is under construction. The major control system tasks required are related to concentrator pointing control, receiver temperature control, and turbine speed control. Attention is given to operational control details, control hardware and software, and aspects of CRT output display.

Factors affecting the availability of americium-241 to the rice plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adriano, D.C.

1979-11-01

Since there has been no published transuranic uptake data on the rice plant (Oryza sativa L.), greenhouse experiments were conducted to determine the effects of some factors on the uptake of /sup 241/Am by this crop. Results indicate that chelated /sup 241/Am (in the form of americium-241-diethylenetriaminepentaacetic acid) applied to the flood water was markedly taken up by the rice plant, compared to the nonchelated form. However, most of the accumulation of /sup 241/Am occurred in the vegetative parts and only trace amounts, if any, were translocated to the grain. Soil application of /sup 241/Am resulted in much lower uptake.more » Soil amendment with either diethylenetriaminepentaacetic acid (DTPA) or organic matter did not produce a discernible uptake pattern. A synthesis of published data on plant uptake of /sup 241/Am indicates that the concentration ratio (CR, a measure of availability of /sup 241/Am to the plants) values for /sup 241/Am for agricultural crops ranged from 10-/sup 6/ to 10/sup 1/ (from lowest to highest availability). Some factors that appear to influence /sup 241/Am uptake are as follows: plant parts (grain usually having lower CR), chelating agents (DTPA usually increasing the CR), organic matter (inconsistent effects although generally decreasing the CR), and lime (usually decreasing the CR).« less

The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments.

PubMed

Martins, Marina C M; Caldana, Camila; Wolf, Lucia Daniela; de Abreu, Luis Guilherme Furlan

2018-01-01

The output of metabolomics relies to a great extent upon the methods and instrumentation to identify, quantify, and access spatial information on as many metabolites as possible. However, the most modern machines and sophisticated tools for data analysis cannot compensate for inappropriate harvesting and/or sample preparation procedures that modify metabolic composition and can lead to erroneous interpretation of results. In addition, plant metabolism has a remarkable degree of complexity, and the number of identified compounds easily surpasses the number of samples in metabolomics analyses, increasing false discovery risk. These aspects pose a large challenge when carrying out plant metabolomics experiments. In this chapter, we address the importance of a proper experimental design taking into consideration preventable complications and unavoidable factors to achieve success in metabolomics analysis. We also focus on quality control and standardized procedures during the metabolomics workflow.

Effect of plants in constructed wetlands for organic carbon and nutrient removal: a review of experimental factors contributing to higher impact and suggestions for future guidelines.

PubMed

Jesus, João M; Danko, Anthony S; Fiúza, António; Borges, Maria-Teresa

2018-02-01

Constructed wetland is a proven technology for water pollution removal, but process mechanisms and their respective contribution are not fully understood. The present review details the effect of plants on removal efficiency of constructed wetlands by focusing on literature that includes experiments with unplanted controls for organic carbon and nutrient (N and P) removal. The contribution of plant direct uptake is also assessed. Although it was found that several studies, mostly at laboratory or pilot scales, showed no statistical differences between planted and unplanted controls, some factors were found that help maximize the effect of plants. This study intends to contribute to a better understanding of the significance of the effect of plants in a constructed wetland, as well as to suggest a set of experimental guidelines in this field.

Parasitic plants in agriculture: Chemical ecology of germination and host-plant location as targets for sustainable control: A review

Treesearch

Justin B. Runyon; John F. Tooker; Mark C. Mescher; Consuelo M. De Moraes

2009-01-01

Parasitic plants are among the most problematic pests of agricultural crops worldwide. Effective means of control are generally lacking, in part because of the close physiological connection between the established parasite and host plant hindering efficient control using traditional methods. Seed germination and host location are critical early-growth stages that...

Capsicum annuum homeobox 1 (CaHB1) is a nuclear factor that has roles in plant development, salt tolerance, and pathogen defense

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oh, Sang-Keun; Yoon, Joonseon; Choi, Gyung Ja

Highlights: •The CaHB1 is a nuclear factor, belonging to HD-Zip proteins. •SA and ET, as signal molecules, modulate CaHB1-mediated responses. •Overexpression of CaHB1 in tomato resulted in a thicker cell wall. •CaHB1-transgenic tomato confers resistance to Phytophthora infestans. •CaHB1 enhanced tolerance to saline stress in tomato. -- Abstract: Homeodomain-leucine zipper (HD-Zip) family proteins are unique to plants, but little is known about their role in defense responses. CaHB1 is a nuclear factor in peppers, belonging to subfamily II of HD-Zip proteins. Here, we determined the role of CaHB1 in the defense response. CaHB1 expression was induced when pepper plants weremore » challenged with Phytophthora capsici, a plant pathogen to which peppers are susceptible, or environmental stresses such as drought and salt stimuli. CaHB1 was also highly expressed in pepper leaves following application of SA, whereas ethephon and MeJA had a moderate effect. To further investigate the function of CaHB1 in plants, we performed gain-of-function study by overexpression of CaHB1 in tomato. CaHB1-transgenic tomatoes showed significant growth enhancement including increased leaf thickness and enlarged cell size (1.8-fold larger than control plants). Microscopic analysis revealed that leaves from CaHB1-transgenic plants had thicker cell walls and cuticle layers than those from controls. Moreover, CaHB1-transgenic plants displayed enhanced resistance against Phytophthora infestans and increased tolerance to salt stress. Additionally, RT-PCR analysis of CaHB1-transgenic tomatoes revealed constitutive up-regulation of multiple genes involved in plant defense and osmotic stress. Therefore, our findings suggest roles for CaHB1 in development, salt stress, and pathogen defense.« less

Analyzing factors that influence the folk use and phytonomy of 18 medicinal plants in Navarra

PubMed Central

Akerreta, Silvia; Cavero, Rita Yolanda; López, Víctor; Calvo, María Isabel

2007-01-01

Background This article analyzes whether the distribution or area of use of 18 medicinal plants is influenced by ecological and cultural factors which might account for their traditional use and/or phytonymy in Navarra. This discussion may be helpful for comparative studies, touching as it does on other ethnopharmacological issues: a) which cultural and ecological factors affect the selection of medicinal plants; b) substitutions of medicinal plants in popular medicine; c) the relation between local nomenclature and uses. To analyze these questions, this paper presents an example of a species used for digestive disorders (tea and camomile: Jasonia glutinosa, J. tuberosa, Sideritis hyssopifolia, Bidens aurea, Chamaemelum nobile, Santolina chamaecyparissus...), high blood pressure (Rhamnus alaternus, Olea europaea...) or skin diseases (Hylotelephium maximum, H. telephium, Anagallis arvensis, A. foemina). Methods Fieldwork began on January 2004 and continued until December 2006. During that time we interviewed 505 informants in 218 locations in Navarra. Information was collected using semi-structured ethnobotanical interviews, and we subsequently made maps using Arc-View 8.0 program to determine the area of use of each taxon. Each map was then compared with the bioclimatic and linguistic map of Navarra, using the soil and ethnographic data for the region, and with other ethnobotanical and ethnopharmacological studies carried out in Europe. Results The results clearly show that ecological and cultural factors influence the selection of medicinal plants in this region. Climate and substrate are the most important ecological factors that influence the distribution and abundance of plants, which are the biological factors that affect medicinal plant selection. Conclusion The study of edaphological and climatological factors, on the one hand, and culture, on the other, can help us to understand why a plant is replaced by another one for the same purposes, either in the

What are the most crucial soil factors for predicting the distribution of alpine plant species?

NASA Astrophysics Data System (ADS)

Buri, A.; Pinto-Figueroa, E.; Yashiro, E.; Guisan, A.

2017-12-01

Nowadays the use of species distribution models (SDM) is common to predict in space and time the distribution of organisms living in the critical zone. The realized environmental niche concept behind the development of SDM imply that many environmental factors must be accounted for simultaneously to predict species distributions. Climatic and topographic factors are often primary included, whereas soil factors are frequently neglected, mainly due to the paucity of soil information available spatially and temporally. Furthermore, among existing studies, most included soil pH only, or few other soil parameters. In this study we aimed at identifying what are the most crucial soil factors for explaining alpine plant distributions and, among those identified, which ones further improve the predictive power of plant SDMs. To test the relative importance of the soil factors, we performed plant SDMs using as predictors 52 measured soil properties of various types such as organic/inorganic compounds, chemical/physical properties, water related variables, mineral composition or grain size distribution. We added them separately to a standard set of topo-climatic predictors (temperature, slope, solar radiation and topographic position). We used ensemble forecasting techniques combining together several predictive algorithms to model the distribution of 116 plant species over 250 sites in the Swiss Alps. We recorded the variable importance for each model and compared the quality of the models including different soil proprieties (one at a time) as predictors to models having only topo-climatic variables as predictors. Results show that 46% of the soil proprieties tested become the second most important variable, after air temperature, to explain spatial distribution of alpine plants species. Moreover, we also assessed that addition of certain soil factors, such as bulk soil water density, could improve over 80% the quality of some plant species models. We confirm that soil p

PlantTFDB 3.0: a portal for the functional and evolutionary study of plant transcription factors

PubMed Central

Jin, Jinpu; Zhang, He; Kong, Lei; Gao, Ge; Luo, Jingchu

2014-01-01

With the aim to provide a resource for functional and evolutionary study of plant transcription factors (TFs), we updated the plant TF database PlantTFDB to version 3.0 (http://planttfdb.cbi.pku.edu.cn). After refining the TF classification pipeline, we systematically identified 129 288 TFs from 83 species, of which 67 species have genome sequences, covering main lineages of green plants. Besides the abundant annotation provided in the previous version, we generated more annotations for identified TFs, including expression, regulation, interaction, conserved elements, phenotype information, expert-curated descriptions derived from UniProt, TAIR and NCBI GeneRIF, as well as references to provide clues for functional studies of TFs. To help identify evolutionary relationship among identified TFs, we assigned 69 450 TFs into 3924 orthologous groups, and constructed 9217 phylogenetic trees for TFs within the same families or same orthologous groups, respectively. In addition, we set up a TF prediction server in this version for users to identify TFs from their own sequences. PMID:24174544

Addressing the human factors issues associated with control room modifications

DOE Office of Scientific and Technical Information (OSTI.GOV)

O`Hara, J.; Stubler, W.; Kramer, J.

1998-03-01

Advanced human-system interface (HSI) technology is being integrated into existing nuclear plants as part of plant modifications and upgrades. The result of this trend is that hybrid HSIs are created, i.e., HSIs containing a mixture of conventional (analog) and advanced (digital) technology. The purpose of the present research is to define the potential effects of hybrid HSIs on personnel performance and plant safety and to develop human factors guidance for safety reviews of them where necessary. In support of this objective, human factors issues associated with hybrid HSIs were identified. The issues were evaluated for their potential significance to plantmore » safety, i.e., their human performance concerns have the potential to compromise plant safety. The issues were then prioritized and a subset was selected for design review guidance development.« less

How the Plant Temperature Links to the Air Temperature in the Desert Plant Artemisia ordosica.

PubMed

Yu, Ming-Han; Ding, Guo-Dong; Gao, Guang-Lei; Sun, Bao-Ping; Zhao, Yuan-Yuan; Wan, Li; Wang, De-Ying; Gui, Zi-Yang

2015-01-01

Plant temperature (Tp) is an important indicator of plant health. To determine the dynamics of plant temperature and self-cooling ability of the plant, we measured Tp in Artemisia ordosica in July, in the Mu Us Desert of Northwest China. Related factors were also monitored to investigate their effects on Tp, including environmental factors, such as air temperature (Ta), relative humidity, wind speed; and physiological factors, such as leaf water potential, sap flow, and water content. The results indicate that: 1) Tp generally changes in conjunction with Ta mainly, and varies with height and among the plant organs. Tp in the young branches is most constant, while it is the most sensitive in the leaves. 2) Correlations between Tp and environmental factors show that Tp is affected mainly by Ta. 3) The self-cooling ability of the plant was effective by midday, with Tp being lower than Ta. 4) Increasing sap flow and leaf water potential showed that transpiration formed part of the mechanism that supported self-cooling. Increased in water conductance and specific heat at midday may be additional factors that contribute to plant cooling ability. Therefore, our results confirmed plant self-cooling ability. The response to high temperatures is regulated by both transpiration speed and an increase in stem water conductance. This study provides quantitative data for plant management in terms of temperature control. Moreover, our findings will assist species selection with taking plant temperature as an index.

ABI-like transcription factor gene TaABL1 from wheat improves multiple abiotic stress tolerances in transgenic plants.

PubMed

Xu, Dong-Bei; Gao, Shi-Qing; Ma, You-Zhi; Xu, Zhao-Shi; Zhao, Chang-Ping; Tang, Yi-Miao; Li, Xue-Yin; Li, Lian-Cheng; Chen, Yao-Feng; Chen, Ming

2014-12-01

The phytohormone abscisic acid (ABA) plays crucial roles in adaptive responses of plants to abiotic stresses. ABA-responsive element binding proteins (AREBs) are basic leucine zipper transcription factors that regulate the expression of downstream genes containing ABA-responsive elements (ABREs) in promoter regions. A novel ABI-like (ABA-insensitive) transcription factor gene, named TaABL1, containing a conserved basic leucine zipper (bZIP) domain was cloned from wheat. Southern blotting showed that three copies were present in the wheat genome. Phylogenetic analyses indicated that TaABL1 belonged to the AREB subfamily of the bZIP transcription factor family and was most closely related to ZmABI5 in maize and OsAREB2 in rice. Expression of TaABL1 was highly induced in wheat roots, stems, and leaves by ABA, drought, high salt, and low temperature stresses. TaABL1 was localized inside the nuclei of transformed wheat mesophyll protoplast. Overexpression of TaABL1 enhanced responses of transgenic plants to ABA and hastened stomatal closure under stress, thereby improving tolerance to multiple abiotic stresses. Furthermore, overexpression of TaABL1 upregulated or downregulated the expression of some stress-related genes controlling stomatal closure in transgenic plants under ABA and drought stress conditions, suggesting that TaABL1 might be a valuable genetic resource for transgenic molecular breeding.

RNAi control of aflatoxins in peanut plants, a multifactorial system

USDA-ARS?s Scientific Manuscript database

RNA-interference (RNAi)-mediated control of aflatoxin contamination in peanut plants is a multifactorial and hyper variable system. The use of RNAi biotechnology to silence single genes in plants has inherently high-variability among transgenic events. Also the level of expression of small interfe...

Trichoderma-plant-pathogen interactions: advances in genetics of biological control.

PubMed

Mukherjee, Mala; Mukherjee, Prasun K; Horwitz, Benjamin A; Zachow, Christin; Berg, Gabriele; Zeilinger, Susanne

2012-12-01

Trichoderma spp. are widely used in agriculture as biofungicides. Induction of plant defense and mycoparasitism (killing of one fungus by another) are considered to be the most important mechanisms of Trichoderma-mediated biological control. Understanding these mechanisms at the molecular level would help in developing strains with superior biocontrol properties. In this article, we review our current understanding of the genetics of interactions of Trichoderma with plants and plant pathogens.

Three phase power factor controller

NASA Technical Reports Server (NTRS)

Nola, F. J. (Inventor)

1984-01-01

A power control circuit for a three phase induction motor is described. Power factors for the three phases are summed to provide a control signal, and this control signal is particularly filtered and then employed to control the duty cycle of each phase of input power to the motor.

SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth.

PubMed

Huai, Junling; Zhang, Xinyu; Li, Jialong; Ma, Tingting; Zha, Ping; Jing, Yanjun; Lin, Rongcheng

2018-05-02

Plants continuously monitor environmental conditions (such as light and temperature) and adjust their growth and development accordingly. The transcription factor PHYTOCHROME-INTERACTING FACTOR4 (PIF4) regulates both light and temperature signaling pathways. Here, we identified ENHANCED PHOTOMORPHOGENIC2 (EPP2) as a new repressor of photomorphogenesis in red, far-red, and blue light. Map-based cloning revealed that EPP2 encodes the SEUSS (SEU) transcription regulator. The C-terminus of SEU has transcriptional activation activity and SEU physically interacts with PIF4. Moreover, SEU promotes the expression of many genes, including auxin biosynthetic and responsive genes, and regulates IAA levels in plants. SEU associates with regulatory regions in INDOLE-3-ACETIC ACID INDUCIBLE6 (IAA6) and IAA19 in a PIF4-independent manner, whereas the binding of PIF4 to these genes requires SEU. Furthermore, mutations in SEU affect H3K4me3 methylation at IAA6 and IAA19, and SEU positively regulates warm temperature-mediated hypocotyl growth together with PIF4. Therefore, our results reveal that SEU acts as a central regulator to integrate light and temperature signals to control plant growth by coordinating with PIF4. Copyright © 2018 The Author. Published by Elsevier Inc. All rights reserved.

Characterization of Phytochrome Interacting Factors from the Moss Physcomitrella patens Illustrates Conservation of Phytochrome Signaling Modules in Land Plants

PubMed Central

Xu, Tengfei; Paik, Inyup; Hanke, Sebastian; Keim, Sarah; Hermann, Helen-Maria; Wolf, Luise; Becker, Claude

2017-01-01

Across the plant kingdom, phytochrome (PHY) photoreceptors play an important role during adaptive and developmental responses to light. In Arabidopsis thaliana, light-activated PHYs accumulate in the nucleus, where they regulate downstream signaling components, such as phytochrome interacting factors (PIFs). PIFs are transcription factors that act as repressors of photomorphogenesis; their inhibition by PHYs leads to substantial changes in gene expression. The nuclear function of PHYs, however, has so far been investigated in only a few non-seed plants. Here, we identified putative target genes of PHY signaling in the moss Physcomitrella patens and found light-regulated genes that are putative orthologs of PIF-controlled genes in Arabidopsis. Phylogenetic analyses revealed that an ancestral PIF-like gene was already present in streptophyte algae, i.e., before the water-to-land transition of plants. The PIF homologs in the genome of P. patens resemble Arabidopsis PIFs in their protein domain structure, molecular properties, and physiological effects, albeit with notable differences in the motif-dependent PHY interaction. Our results suggest that P. patens PIFs are involved in PHY signaling. The PHY-PIF signaling node that relays light signals to target genes has been largely conserved during land plant evolution, with evidence of lineage-specific diversification. PMID:28123107

Assessment of destabilizing factor for automatic control systems in propulsion systems of mechatronic and maritime transport objects

NASA Astrophysics Data System (ADS)

Zhilenkov, A. A.; Kapitonov, A. A.

2017-10-01

It is known that many of today’s ships and vessels have a shaft generator as a part of their power plants. Modern automatic control systems used in the world’s fleet do not enable their shaft generators to operate in parallel with the main diesel generators for long-term sustenance of the total load of the ship network. On the other hand, according to our calculations and experiments, a shaft generator operated in parallel with the main power plant helps save at least 10% of fuel while making the power system of the ship more efficient, reliable, and eco-friendly. The fouling and corrosion of the propeller as well as the weather conditions of navigation affect its modulus of resistance. It changes the free component of the transient process of shaft generator stress frequency changes in transient processes. While the shaft generator and the diesel generator of the ship power plant are paralleled, there emerges an angle between their EMF. This results in equalizing currents generated between them. The altering torque in the drive-shaft line—propeller system causes torsional fluctuations of the ship shaft line. To compensate for the effect of destabilizing factors and torsional fluctuations of the shaft line on the dynamic characteristics of the transient process that alters the RPM of the main engine, sliding mode controls can be used. To synthesize such a control, one has to evaluate the effect of destabilizing factors.

Modeling, simulation, and high-autonomy control of a Martian oxygen production plant

NASA Technical Reports Server (NTRS)

Schooley, L. C.; Cellier, F. E.; Wang, F.-Y.; Zeigler, B. P.

1992-01-01

Progress on a project for the development of a high-autonomy intelligent command and control architecture for process plants used to produce oxygen from local planetary resources is reported. A distributed command and control architecture is being developed and implemented so that an oxygen production plant, or other equipment, can be reliably commanded and controlled over an extended time period in a high-autonomy mode with high-level task-oriented teleoperation from one or several remote locations. During the reporting period, progress was made at all levels of the architecture. At the remote site, several remote observers can now participate in monitoring the plant. At the local site, a command and control center was introduced for increased flexibility, reliability, and robustness. The local control architecture was enhanced to control multiple tubes in parallel, and was refined for increased robustness. The simulation model was enhanced to full dynamics descriptions.

The role of plant-microbiome interactions in weed establishment and control.

PubMed

Trognitz, Friederike; Hackl, Evelyn; Widhalm, Siegrid; Sessitsch, Angela

2016-10-01

The soil microbiome plays an important role in the establishment of weeds and invasive plants. They associate with microorganisms supporting their growth and health. Weed management strategies, like tillage and herbicide treatments, to control weeds generally alter soil structure going alongside with changes in the microbial community. Once a weed population establishes in the field, the plants build up a close relationship with the available microorganisms. Seeds or vegetative organs overwinter in soil and select early in the season their own microbiome before crop plants start to vegetate. Weed and crop plants compete for light, nutrition and water, but may differently interact with soil microorganisms. The development of new sequencing technologies for analyzing soil microbiomes has opened up the possibility for in depth analysis of the interaction between 'undesired' plants and crop plants under different management systems. These findings will help us to understand the functions of microorganisms involved in crop productivity and plant health, weed establishment and weed prevention. Exploitation of the knowledge offers the possibility to search for new biocontrol methods against weeds based on soil and plant-associated microorganisms. This review discusses the recent advances in understanding the functions of microbial communities for weed/invasive plant establishment and shows new ways to use plant-associated microorganisms to control weeds and invasive plants in different land management systems. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

50 CFR 35.7 - Control of wildfires, insects, pest plants, and disease.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., and disease. 35.7 Section 35.7 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... MANAGEMENT General Rules § 35.7 Control of wildfires, insects, pest plants, and disease. To the extent necessary, the Director shall prescribe measures to control wildfires, insects, pest plants, and disease to...

50 CFR 35.7 - Control of wildfires, insects, pest plants, and disease.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., and disease. 35.7 Section 35.7 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... MANAGEMENT General Rules § 35.7 Control of wildfires, insects, pest plants, and disease. To the extent necessary, the Director shall prescribe measures to control wildfires, insects, pest plants, and disease to...

50 CFR 35.7 - Control of wildfires, insects, pest plants, and disease.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., and disease. 35.7 Section 35.7 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... MANAGEMENT General Rules § 35.7 Control of wildfires, insects, pest plants, and disease. To the extent necessary, the Director shall prescribe measures to control wildfires, insects, pest plants, and disease to...

50 CFR 35.7 - Control of wildfires, insects, pest plants, and disease.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., and disease. 35.7 Section 35.7 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... MANAGEMENT General Rules § 35.7 Control of wildfires, insects, pest plants, and disease. To the extent necessary, the Director shall prescribe measures to control wildfires, insects, pest plants, and disease to...

50 CFR 35.7 - Control of wildfires, insects, pest plants, and disease.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., and disease. 35.7 Section 35.7 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... MANAGEMENT General Rules § 35.7 Control of wildfires, insects, pest plants, and disease. To the extent necessary, the Director shall prescribe measures to control wildfires, insects, pest plants, and disease to...

Planting pattern and weed control method influence on yield production of corn (Zea mays L.)

NASA Astrophysics Data System (ADS)

Purba, E.; Nasution, D. P.

2018-02-01

Field experiment was carried out to evaluate the influence of planting patterns and weed control methods on the growth and yield of corn. The effect of the planting pattern and weed control method was studied in a split plot design. The main plots were that of planting pattern single row (25cm x 60cm), double row (25cm x 25cm x 60cm) and triangle row ( 25cm x 25cm x 25cm). Subplot was that of weed control method consisted five methods namely weed free throughout the growing season, hand weeding, sprayed with glyphosate, sprayed with paraquat, and no weeding.. Result showed that both planting pattern and weed control method did not affect the growth of corn. However, planting pattern and weed control method significantly affected yield production. Yield resulted from double row and triangle planting pattern was 14% and 41% higher, consecutively, than that of single row pattern. The triangle planting pattern combined with any weed control method produced the highest yield production of corn.

A Controlled Environment System For Measuring Plant-Atmosphere Gas Exchange

Treesearch

James M. Brown

1975-01-01

Describes an inexpensive, efficient system for measuring plant-atmosphere gas exchange. Designed to measure transpiration from potted tree seedlings, it is readily adaptable for measuring other gas exchanges or gas exchange by plant parts. Light level, air and root temperature can be precisely controlled at minimum cost.

PlantTFDB 4.0: toward a central hub for transcription factors and regulatory interactions in plants.

PubMed

Jin, Jinpu; Tian, Feng; Yang, De-Chang; Meng, Yu-Qi; Kong, Lei; Luo, Jingchu; Gao, Ge

2017-01-04

With the goal of providing a comprehensive, high-quality resource for both plant transcription factors (TFs) and their regulatory interactions with target genes, we upgraded plant TF database PlantTFDB to version 4.0 (http://planttfdb.cbi.pku.edu.cn/). In the new version, we identified 320 370 TFs from 165 species, presenting a more comprehensive genomic TF repertoires of green plants. Besides updating the pre-existing abundant functional and evolutionary annotation for identified TFs, we generated three new types of annotation which provide more directly clues to investigate functional mechanisms underlying: (i) a set of high-quality, non-redundant TF binding motifs derived from experiments; (ii) multiple types of regulatory elements identified from high-throughput sequencing data; (iii) regulatory interactions curated from literature and inferred by combining TF binding motifs and regulatory elements. In addition, we upgraded previous TF prediction server, and set up four novel tools for regulation prediction and functional enrichment analyses. Finally, we set up a novel companion portal PlantRegMap (http://plantregmap.cbi.pku.edu.cn) for users to access the regulation resource and analysis tools conveniently. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

The sunflower transcription factor HaHB11 confers tolerance to water deficit and salinity to transgenic Arabidopsis and alfalfa plants.

PubMed

Cabello, Julieta V; Giacomelli, Jorge I; Gómez, María C; Chan, Raquel L

2017-09-10

Homeodomain-leucine zipper (HD-Zip) transcription factors are unique to the plant kingdom; members of subfamily I are known to be involved in abiotic stress responses. HaHB11 belongs to this subfamily and it was previously shown that it is able to confer improved yield and tolerance to flooding via a quiescent strategy. Here we show that HaHB11 expression is induced by ABA, NaCl and water deficit in sunflower seedlings and leaves. Arabidopsis transgenic plants expressing HaHB11, controlled either by its own promoter or by the constitutive 35S CaMV, presented rolled leaves and longer roots than WT when grown under standard conditions. In addition, these plants showed wider stems and more vascular bundles. To deal with drought, HaHB11 transgenic plants closed their stomata faster and lost less water than controls, triggering an enhanced tolerance to such stress condition and also to salinity stress. Concomitantly, ABA-synthesis and sensing related genes were differentially regulated in HaHB11 transgenic plants. Either under long-term salinity stress or mild drought stress, HaHB11 transgenic plants did not exhibit yield penalties. Moreover, alfalfa transgenic plants were generated which also showed enhanced drought tolerance. Altogether, the results indicated that HaHB11 was able to confer drought and salinity tolerance via a complex mechanism which involves morphological, physiological and molecular changes. Copyright © 2016 Elsevier B.V. All rights reserved.

NAD Kinases: Metabolic Targets Controlling Redox Co-enzymes and Reducing Power Partitioning in Plant Stress and Development

PubMed Central

Li, Bin-Bin; Wang, Xiang; Tai, Li; Ma, Tian-Tian; Shalmani, Abdullah; Liu, Wen-Ting; Li, Wen-Qiang; Chen, Kun-Ming

2018-01-01

NAD(H) and NADP(H) are essential co-enzymes which dominantly control a number of fundamental biological processes by acting as reducing power and maintaining the intracellular redox balance of all life kingdoms. As the only enzymes that catalyze NAD(H) and ATP to synthesize NADP(H), NAD Kinases (NADKs) participate in many essential metabolic reactions, redox sensitive regulation, photosynthetic performance and also reactive oxygen species (ROS) homeostasis of cells and therefore, play crucial roles in both development and stress responses of plants. NADKs are highly conserved enzymes in amino acid sequences but have multiple subcellular localization and diverse functions. They may function as monomers, dimers or multimers in cells but the enzymatic properties in plants are not well elucidated yet. The activity of plant NADK is regulated by calcium/calmodulin and plays crucial roles in photosynthesis and redox co-enzyme control. NADK genes are expressed in almost all tissues and developmental stages of plants with specificity for different members. Their transcripts can be greatly stimulated by a number of environmental factors such as pathogenic attack, irritant applications and abiotic stress treatments. Using transgenic approaches, several studies have shown that NADKs are involved in chlorophyll synthesis, photosynthetic efficiency, oxidative stress protection, hormone metabolism and signaling regulation, and therefore contribute to the growth regulation and stress tolerance of plants. In this review, the enzymatic properties and functional mechanisms of plant NADKs are thoroughly investigated based on literature and databases. The results obtained here are greatly advantageous for further exploration of NADK function in plants. PMID:29662499

Trichoderma asperellum strain T34 controls Fusarium wilt disease in tomato plants in soilless culture through competition for iron.

PubMed

Segarra, Guillem; Casanova, Eva; Avilés, Manuel; Trillas, Isabel

2010-01-01

Trichoderma asperellum strain T34 has been reported to control the disease caused by Fusarium oxysporum f.sp. lycopersici (Fol) on tomato plants. To study the importance of iron concentration in the growth media for the activity and competitiveness of T34 and the pathogen, we tested four iron concentrations in the nutrient solution [1, 10, 100, and 1000 microM provided as EDTA/Fe(III)] in a biological control experiment with T34 and Fol in tomato plants. The reduction of the Fusarium-infected shoot by T34 was only significant at 10 microM Fe. We hypothesized that Fe competition is one of the key factors in the biocontrol activity exerted by T34 against Fol, as an increase in Fe concentration over 10 microM would lead to the suppression of T34 siderophore synthesis and thus inhibition of Fe competition with Fol. T34 significantly reduced the populations of Fol at all the doses of Fe assayed. In contrast, Fol enhanced the populations of T34 at 1 and 10 microM Fe. Nevertheless, several plant physiological parameters like net CO(2) assimilation (A), stomatal conductance (g(s)), relative quantum efficiency of PSII (Phi(PSII)), and efficiency of excitation energy capture by open PSII reactive centers (Fv'/Fm') demonstrated the protection against Fol damage by treatment with T34 at 100 microM Fe. The first physiological parameter affected by the disease progression was g(s). Plant dry weight was decreased by Fe toxicity at 100 and 1,000 microM. T34-treated plants had significantly greater heights and dry weights than control plants at 1,000 microM Fe, even though T34 did not reduce the Fe content in leaves or stems. Furthermore, T34 enhanced plant height even at the optimal Fe concentration (10 microM) compared to control plants. In conclusion, T. asperellum strain T34 protected tomato plants from both biotic (Fusarium wilt disease) and abiotic stress [Fe(III) toxic effects].

Computer control improves ethylene plant operation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Whitehead, B.D.; Parnis, M.

ICIA Australia ordered a turnkey 250,000-tpy ethylene plant to be built at the Botany site, Sydney, Australia. Following a feasibility study, an additional order was placed for a process computer system for advanced process control and optimization. This article gives a broad outline of the process computer tasks, how the tasks were implemented, what problems were met, what lessons were learned and what results were achieved.

β-amylase-like proteins function as transcription factors in Arabidopsis, controlling shoot growth and development.

PubMed

Reinhold, Heike; Soyk, Sebastian; Simková, Klára; Hostettler, Carmen; Marafino, John; Mainiero, Samantha; Vaughan, Cara K; Monroe, Jonathan D; Zeeman, Samuel C

2011-04-01

Plants contain β-amylase-like proteins (BAMs; enzymes usually associated with starch breakdown) present in the nucleus rather than targeted to the chloroplast. They possess BRASSINAZOLE RESISTANT1 (BZR1)-type DNA binding domains--also found in transcription factors mediating brassinosteroid (BR) responses. The two Arabidopsis thaliana BZR1-BAM proteins (BAM7 and BAM8) bind a cis-regulatory element that both contains a G box and resembles a BR-responsive element. In protoplast transactivation assays, these BZR1-BAMs activate gene expression. Structural modeling suggests that the BAM domain's glucan binding cleft is intact, but the recombinant proteins are at least 1000 times less active than chloroplastic β-amylases. Deregulation of BZR1-BAMs (the bam7bam8 double mutant and BAM8-overexpressing plants) causes altered leaf growth and development. Of the genes upregulated in plants overexpressing BAM8 and downregulated in bam7bam8 plants, many carry the cis-regulatory element in their promoters. Many genes that respond to BRs are inversely regulated by BZR1-BAMs. We propose a role for BZR1-BAMs in controlling plant growth and development through crosstalk with BR signaling. Furthermore, we speculate that BZR1-BAMs may transmit metabolic signals by binding a ligand in their BAM domain, although diurnal changes in the concentration of maltose, a candidate ligand produced by chloroplastic β-amylases, do not influence their transcription factor function.

Maximum plant height and the biophysical factors that limit it.

PubMed

Niklas, Karl J

2007-03-01

Basic engineering theory and empirically determined allometric relationships for the biomass partitioning patterns of extant tree-sized plants show that the mechanical requirements for vertical growth do not impose intrinsic limits on the maximum heights that can be reached by species with woody, self-supporting stems. This implies that maximum tree height is constrained by other factors, among which hydraulic constraints are plausible. A review of the available information on scaling relationships observed for large tree-sized plants, nevertheless, indicates that mechanical and hydraulic requirements impose dual restraints on plant height and thus, may play equally (but differentially) important roles during the growth of arborescent, large-sized species. It may be the case that adaptations to mechanical and hydraulic phenomena have optimized growth, survival and reproductive success rather than longevity and mature size.

Commercialization of the power factor controller

NASA Technical Reports Server (NTRS)

1981-01-01

The purpose of the Motor Power Controller, also known as the Power Factor Controller, is to improve power factor and reduce power dissipation in induction motors operating below full load. These purposes were studied and tested in detail. The Motor Power Controller is capable of raising power factors from 0.2 to 0.8 and results in energy savings. It was found that many motors, in their present operating applications, are classified as unstable. The electronic nature of the controller vs. the electrical nature of the motor, compound this problem due to the differences in response time of the two devices. Many tests were successfully completed, the most indicating greater savings than anticipated. Also, there was an effect on efficiency which was not included in the calculations.

WRKY transcription factors

PubMed Central

Bakshi, Madhunita; Oelmüller, Ralf

2014-01-01

WRKY transcription factors are one of the largest families of transcriptional regulators found exclusively in plants. They have diverse biological functions in plant disease resistance, abiotic stress responses, nutrient deprivation, senescence, seed and trichome development, embryogenesis, as well as additional developmental and hormone-controlled processes. WRKYs can act as transcriptional activators or repressors, in various homo- and heterodimer combinations. Here we review recent progress on the function of WRKY transcription factors in Arabidopsis and other plant species such as rice, potato, and parsley, with a special focus on abiotic, developmental, and hormone-regulated processes. PMID:24492469

Implementing a rational and consistent nomenclature for serine/arginine-rich protein splicing factors (SR proteins) in plants.

PubMed

Barta, Andrea; Kalyna, Maria; Reddy, Anireddy S N

2010-09-01

Growing interest in alternative splicing in plants and the extensive sequencing of new plant genomes necessitate more precise definition and classification of genes coding for splicing factors. SR proteins are a family of RNA binding proteins, which function as essential factors for constitutive and alternative splicing. We propose a unified nomenclature for plant SR proteins, taking into account the newly revised nomenclature of the mammalian SR proteins and a number of plant-specific properties of the plant proteins. We identify six subfamilies of SR proteins in Arabidopsis thaliana and rice (Oryza sativa), three of which are plant specific. The proposed subdivision of plant SR proteins into different subfamilies will allow grouping of paralogous proteins and simple assignment of newly discovered SR orthologs from other plant species and will promote functional comparisons in diverse plant species.

Controlling Herbaceous Competition in Pasture Planted with Loblolly Pine Seedlings

Treesearch

James D. Haywood

1995-01-01

Three treatments designed to control herbaceous vegetation competing with loblolly pine(Pinus taeda L.) seedlings planted in grazed and ungrazed pasture were tested. Effects of the treatments on seedling survival and growth during the first 3 years after planting were determined. The treatments were directed application of herbicides (glyphosate in...

Genetic and epigenetic control of plant heat responses

PubMed Central

Liu, Junzhong; Feng, Lili; Li, Jianming; He, Zuhua

2015-01-01

Plants have evolved sophisticated genetic and epigenetic regulatory systems to respond quickly to unfavorable environmental conditions such as heat, cold, drought, and pathogen infections. In particular, heat greatly affects plant growth and development, immunity and circadian rhythm, and poses a serious threat to the global food supply. According to temperatures exposing, heat can be usually classified as warm ambient temperature (about 22–27°C), high temperature (27–30°C) and extremely high temperature (37–42°C, also known as heat stress) for the model plant Arabidopsis thaliana. The genetic mechanisms of plant responses to heat have been well studied, mainly focusing on elevated ambient temperature-mediated morphological acclimation and acceleration of flowering, modulation of circadian clock and plant immunity by high temperatures, and thermotolerance to heat stress. Recently, great progress has been achieved on epigenetic regulation of heat responses, including DNA methylation, histone modifications, histone variants, ATP-dependent chromatin remodeling, histone chaperones, small RNAs, long non-coding RNAs and other undefined epigenetic mechanisms. These epigenetic modifications regulate the expression of heat-responsive genes and function to prevent heat-related damages. This review focuses on recent progresses regarding the genetic and epigenetic control of heat responses in plants, and pays more attention to the role of the major epigenetic mechanisms in plant heat responses. Further research perspectives are also discussed. PMID:25964789

Reliable and Affordable Control Systems Active Combustor Pattern Factor Control

NASA Technical Reports Server (NTRS)

McCarty, Bob; Tomondi, Chris; McGinley, Ray

2004-01-01

Active, closed-loop control of combustor pattern factor is a cooperative effort between Honeywell (formerly AlliedSignal) Engines and Systems and the NASA Glenn Research Center to reduce emissions and turbine-stator vane temperature variations, thereby enhancing engine performance and life, and reducing direct operating costs. Total fuel flow supplied to the engine is established by the speed/power control, but the distribution to individual atomizers will be controlled by the Active Combustor Pattern Factor Control (ACPFC). This system consist of three major components: multiple, thin-film sensors located on the turbine-stator vanes; fuel-flow modulators for individual atomizers; and control logic and algorithms within the electronic control.

Digital control for the condensate system in a combined cycle power plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sanchez Parra, M.; Fuentes Gutierrez, J.E.; Castelo Cuevas, L.

1994-12-31

This paper presents the highlights by means of which development, installation and start up of the digital control system (DCS)for the condenser and hotwell (condensate system) were performed. This system belongs to the distributed control system installed by the Instituto de Investigaciones Electricas (IIE) at the Combined Cycle Power Plant in Gomez Palacio (GP), Durango, Mexico, during the February-March period, in 1993. The main steps for development of the condenser and hotwell control system include: process modeling, definition of control strategies, algorithms, design and software development, PC simulation tests, laboratory tests with an equipment similar to the one installed atmore » the GP Power Plant, installation, and finally, start up, which was a joint effort with the GP Power Plant engineering staff.« less

Heart Disease Risk Factors You Can't Control

MedlinePlus

... Submit Heart disease risk factors you can't control Some factors you can't control, like pregnancy ... 2018. Heart disease risk factors you can't control Age and menopause As you get older, your ...

Plant management in natural areas: balancing chemical, mechanical, and cultural control methods

Treesearch

Steven Manning; James Miller

2011-01-01

After determining the best course of action for control of an invasive plant population, it is important to understand the variety of methods available to the integrated pest management professional. A variety of methods are now widely used in managing invasive plants in natural areas, including chemical, mechanical, and cultural control methods. Once the preferred...

Effects of Abiotic Factors on HIPV-Mediated Interactions between Plants and Parasitoids

PubMed Central

Becker, Christine; Desneux, Nicolas; Monticelli, Lucie; Fernandez, Xavier; Michel, Thomas; Lavoir, Anne-Violette

2015-01-01

In contrast to constitutively emitted plant volatiles (PV), herbivore-induced plant volatiles (HIPV) are specifically emitted by plants when afflicted with herbivores. HIPV can be perceived by parasitoids and predators which parasitize or prey on the respective herbivores, including parasitic hymenoptera. HIPV act as signals and facilitate host/prey detection. They comprise a blend of compounds: main constituents are terpenoids and “green leaf volatiles.” Constitutive emission of PV is well known to be influenced by abiotic factors like temperature, light intensity, water, and nutrient availability. HIPV share biosynthetic pathways with constitutively emitted PV and might therefore likewise be affected by abiotic conditions. However, the effects of abiotic factors on HIPV-mediated biotic interactions have received only limited attention to date. HIPV being influenced by the plant's growing conditions could have major implications for pest management. Quantitative and qualitative changes in HIPV blends may improve or impair biocontrol. Enhanced emission of HIPV may attract a larger number of natural enemies. Reduced emission rates or altered compositions, however, may render blends imperceptible to parasitoides and predators. Predicting the outcome of these changes is highly important for food production and for ecosystems affected by global climate change. PMID:26788501

Arabidopsis WRKY46, WRKY54, and WRKY70 Transcription Factors Are Involved in Brassinosteroid-Regulated Plant Growth and Drought Responses

PubMed Central

Chen, Jiani; Nolan, Trevor M.; Zhang, Mingcai; Tong, Hongning; Xin, Peiyong; Chu, Jinfang; Li, Zhaohu

2017-01-01

Plant steroid hormones, brassinosteroids (BRs), play important roles in growth and development. BR signaling controls the activities of BRASSINOSTERIOD INSENSITIVE1-EMS-SUPPRESSOR1/BRASSINAZOLE-RESISTANT1 (BES1/BZR1) family transcription factors. Besides the role in promoting growth, BRs are also implicated in plant responses to drought stress. However, the molecular mechanisms by which BRs regulate drought response have just begun to be revealed. The functions of WRKY transcription factors in BR-regulated plant growth have not been established, although their roles in stress responses are well documented. Here, we found that three Arabidopsis thaliana group III WRKY transcription factors, WRKY46, WRKY54, and WRKY70, are involved in both BR-regulated plant growth and drought response as the wrky46 wrky54 wrky70 triple mutant has defects in BR-regulated growth and is more tolerant to drought stress. RNA-sequencing analysis revealed global roles of WRKY46, WRKY54, and WRKY70 in promoting BR-mediated gene expression and inhibiting drought responsive genes. WRKY54 directly interacts with BES1 to cooperatively regulate the expression of target genes. In addition, WRKY54 is phosphorylated and destabilized by GSK3-like kinase BR-INSENSITIVE2, a negative regulator in the BR pathway. Our results therefore establish WRKY46/54/70 as important signaling components that are positively involved in BR-regulated growth and negatively involved in drought responses. PMID:28576847

Status of NO sub x control for coal-fired power plants

NASA Technical Reports Server (NTRS)

Teixeira, D. P.

1978-01-01

The status of technologies for controlling emissions of oxides of nitrogen (NOx) from coal-fired power plants is reviewed. A discussion of current technology as well as future NOx control approaches is presented. Advanced combustion approaches are included as well as post-combustion alternatives such as catalytic and noncatalytic ammonia-bases systems and wet scrubbing. Special emphasis is given to unresolved development issues as they relate to practical applications on coal-fired power plants.

78 FR 38739 - Special Nuclear Material Control and Accounting Systems for Nuclear Power Plants

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-27

... NUCLEAR REGULATORY COMMISSION [NRC-2012-0109] Special Nuclear Material Control and Accounting... Guide (RG) 5.29, ``Special Nuclear Material Control and Accounting Systems for Nuclear Power Plants... material control and accounting. This guide applies to all nuclear power plants. ADDRESSES: Please refer to...

Promise for plant pest control: root-associated pseudomonads with insecticidal activities

PubMed Central

Kupferschmied, Peter; Maurhofer, Monika; Keel, Christoph

2013-01-01

Insects are an important and probably the most challenging pest to control in agriculture, in particular when they feed on belowground parts of plants. The application of synthetic pesticides is problematic owing to side effects on the environment, concerns for public health and the rapid development of resistance. Entomopathogenic bacteria, notably Bacillus thuringiensis and Photorhabdus/Xenorhabdus species, are promising alternatives to chemical insecticides, for they are able to efficiently kill insects and are considered to be environmentally sound and harmless to mammals. However, they have the handicap of showing limited environmental persistence or of depending on a nematode vector for insect infection. Intriguingly, certain strains of plant root-colonizing Pseudomonas bacteria display insect pathogenicity and thus could be formulated to extend the present range of bioinsecticides for protection of plants against root-feeding insects. These entomopathogenic pseudomonads belong to a group of plant-beneficial rhizobacteria that have the remarkable ability to suppress soil-borne plant pathogens, promote plant growth, and induce systemic plant defenses. Here we review for the first time the current knowledge about the occurrence and the molecular basis of insecticidal activity in pseudomonads with an emphasis on plant-beneficial and prominent pathogenic species. We discuss how this fascinating Pseudomonas trait may be exploited for novel root-based approaches to insect control in an integrated pest management framework. PMID:23914197

Control of cellulose biosynthesis by overexpression of a transcription factor

DOEpatents

Han, Kyung-Hwan; Ko, Jae-Heung; Kim, Won-Chan; Kim; , Joo-Yeol

2017-05-16

The invention relates to the over-expression of a transcription factor selected from the group consisting of MYB46, HAM1, HAM2, MYB112, WRKY11, ERF6, and any combination thereof in a plant, which can modulate and thereby modulating the cellulose content of the plant.

Correlative controls of senescence and plant death in Arabidopsis thaliana (Brassicaceae).

PubMed

Noodén, L D; Penney, J P

2001-11-01

Like most monocarpic plants, longevity of Arabidopsis thaliana plants is controlled by the reproductive structures; however, they appear to work differently from most dicots studied. Neither male- and female-sterility mutations (ms1-1 and bell1, respectively) nor surgical removal of the stems with inflorescences (bolts) at various stages significantly increased the longevity of individual rosette leaves, yet the mutants and treated plants lived 20-50 d longer, measured by the death of the last rosette and/or the last cauline leaf. A series of growth mutations (clv2-4, clv3-2, det3, vam1 enh, and dark green) also increased plant longevity by 20-30 d but did not delay the overall development of the plants. The mutations prolonged plant life through the production of new leaves and stems with inflorescences (bolts) rather than by extending leaf longevity. In growing stems, the newly-formed leaves may induce senescence in the older leaves; however, removal of the younger leaves did not significantly increase the life of the older leaves on the compressed stems of Arabidopsis. Since plants that produce more bolts also live longer, the reproductive load (dry weight) of the bolts did not seem to drive leaf or whole plant senescence here. The developing reproductive structures caused the death of the plant by preventing regeneration of leaves and bolts, which are green and presumably photosynthetic. They also exerted a correlative control (repression) on the development of additional reproductive structures.

Plant-based immunocontraceptive control of wildlife--"potentials, limitations, and possums".

PubMed

Polkinghorne, Ian; Hamerli, Denes; Cowan, Phil; Duckworth, Janine

2005-03-07

Possums (Trichosurus vulpecula), originally introduced from Australia, are spread over 90% of New Zealand and cause major economic and environmental damage. Immunocontraception has been suggested as a humane means to control them. Marsupial-specific reproductive antigens expressed at high levels in edible transgenic plant tissue might provide a safe, effective, and cheap oral delivery bait for immunocontraceptive control. As proof of concept, female possums vaccinated with immunocontraceptive antigens showed reduced fertility, and possums fed with potato-expressed heat labile toxin-B (LT-B) had mucosal and systemic immune responses to the antigen. This demonstrated that immunocontraception was effective in possums and that oral delivery in edible plant material might be possible. Nuclear transformation with reporter genes showed that transgenic carrot roots accumulate high levels of foreign protein in edible tissues, indicating their potential as a delivery vector. However, prior to attempts at large scale production, more effective immunocontraceptive antigen-adjuvant formulations are probably required before plant-based immunocontraception can become a major tool for immunocontraceptive control of overabundant vertebrate pests.

The influence of space flight factors on viability and mutability of plants.

PubMed

Kostina, L; Anikeeva, I; Vaulina, E

1984-01-01

The experiments with air-dried Crepis capillaris seeds aboard the Soyuz 16 spaceship and the orbital stations Salyut 5, 6, 7 have revealed an increase in the frequency of aberrant cells in seedlings grown from flight-exposed seeds during the flight (experiment) and after the flight on Earth (flight control) as compared to the ground-based control. The increase in seedlings grown during the flight is more significant than in the flight control. During the flight Arabidopsis thaliana developed from cotyledons to the flowering stage. Analysis of seeds setting on these plants after the flight has shown a reduction in the fertility of these plants and an increase in the frequency of recessive mutants ("Light block-1"). An increased frequency of mutants was also retained in the progeny of plants which had passed through a complete cycle of development during the flight ("Fiton-3"). Suppression of embryo viability was observed in all experiments and expressed itself in reduced germinating ability of seeds from the exposed plants and in the early death of seedlings. Damages resulting from chromosome aberrations are eliminated in the first postflight generation and damages resulting from gene mutations and micro-aberrations are preserved for a longer time.

Control of Plant Trichome and Root-Hair Development by a Tomato (Solanum lycopersicum) R3 MYB Transcription Factor

PubMed Central

Tominaga-Wada, Rumi; Nukumizu, Yuka; Sato, Shusei; Wada, Takuji

2013-01-01

In Arabidopsis thaliana the CPC-like MYB transcription factors [CAPRICE (CPC), TRIPTYCHON (TRY), ENHANCER OF TRY AND CPC 1, 2, 3/CPC-LIKE MYB 3 (ETC1, ETC2, ETC3/CPL3), TRICHOMELESS 1, 2/CPC-LIKE MYB 4 (TCL1, TCL2/CPL4)] and the bHLH transcription factors [GLABRA3 (GL3) and ENHANCER OF GLABRA 3 (EGL3)] are central regulators of trichome and root-hair development. We identified TRY and GL3 homologous genes from the tomato genome and named them SlTRY and SlGL3, respectively. Phylogenic analyses revealed a close relationship between the tomato and Arabidopsis genes. Real-time reverse transcription PCR analyses showed that SlTRY and SlGL3 were predominantly expressed in aerial parts of developing tomato. After transformation into Arabidopsis, CPC::SlTRY inhibited trichome formation and enhanced root-hair differentiation by strongly repressing GL2 expression. On the other hand, GL3::SlGL3 transformation did not show any obvious effect on trichome or non-hair cell differentiation. These results suggest that tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation, and that a CPC-like R3 MYB may be a key common regulator of plant trichome and root-hair development. PMID:23326563

Driving factors of the communities of phytophagous and predatory mites in a physic nut plantation and spontaneous plants associated.

PubMed

Cruz, Wilton P; Sarmento, Renato A; Teodoro, Adenir V; Neto, Marçal P; Ignacio, Maíra

2013-08-01

Seasonal changes in climate and plant diversity are known to affect the population dynamics of both pests and natural enemies within agroecosystems. In Brazil, spontaneous plants are usually tolerated in small-scale physic nut plantations over the year, which in turn may mediate interactions between pests and natural enemies within this agroecosystem. Here, we aimed to access the influence of seasonal variation of abiotic (temperature, relative humidity and rainfall) and biotic (diversity of spontaneous plants, overall richness and density of mites) factors on the communities of phytophagous and predatory mites found in a physic nut plantation and its associated spontaneous plants. Mite sampling was monthly conducted in dicotyledonous and monocotyledonous leaves of spontaneous plants as well as in physic nut shrubs over an entire year. In the dry season there was a higher abundance of phytophagous mites (Tenuipalpidae, Tarsonemidae and Tetranychidae) on spontaneous plants than on physic nut shrubs, while predatory mites (Phytoseiidae) showed the opposite pattern. The overall density of mites on spontaneous plants increased with relative humidity and diversity of spontaneous plants. Rainfall was the variable that most influenced the density of mites inhabiting physic nut shrubs. Agroecosystems comprising spontaneous plants associated with crops harbour a rich mite community including species of different trophic levels which potentially benefit natural pest control due to increased diversity and abundance of natural enemies.

Melatonin: A Multifunctional Factor in Plants

PubMed Central

Fan, Jibiao; Zhang, Zaichao; Chen, Liang

2018-01-01

Melatonin (N-acetyl-5-methoxy-tryptamine) is a universal molecule that is present in animals and plants. It has been detected in different kinds of plants and organs in different levels. Melatonin in plants shares the same initial biosynthesis compound with auxin, and therefore functions as indole-3-acetic acid like hormones. Moreover, melatonin is involved in regulating plant growth and development, protecting plants against biotic and abiotic stresses, such as salt, drought, cold, heat and heavy metal stresses. Melatonin improves the stress tolerance of plants via a direct pathway, which scavenges reactive oxygen species directly, and indirect pathways, such as increasing antioxidate enzymes activity, photosynthetic efficiency and metabolites content. In addition, melatonin plays a role in regulating gene expression, and hence affects performance of plants. In this review, the biosynthesis pathway, growth and development regulation, and the environment stress response of melatonin in plants are summarized and future research directions and priorities of melatonin in plants are speculated. PMID:29883400

Melatonin: A Multifunctional Factor in Plants.

PubMed

Fan, Jibiao; Xie, Yan; Zhang, Zaichao; Chen, Liang

2018-05-21

Melatonin ( N -acetyl-5-methoxy-tryptamine) is a universal molecule that is present in animals and plants. It has been detected in different kinds of plants and organs in different levels. Melatonin in plants shares the same initial biosynthesis compound with auxin, and therefore functions as indole-3-acetic acid like hormones. Moreover, melatonin is involved in regulating plant growth and development, protecting plants against biotic and abiotic stresses, such as salt, drought, cold, heat and heavy metal stresses. Melatonin improves the stress tolerance of plants via a direct pathway, which scavenges reactive oxygen species directly, and indirect pathways, such as increasing antioxidate enzymes activity, photosynthetic efficiency and metabolites content. In addition, melatonin plays a role in regulating gene expression, and hence affects performance of plants. In this review, the biosynthesis pathway, growth and development regulation, and the environment stress response of melatonin in plants are summarized and future research directions and priorities of melatonin in plants are speculated.

DSC: software tool for simulation-based design of control strategies applied to wastewater treatment plants.

PubMed

Ruano, M V; Ribes, J; Seco, A; Ferrer, J

2011-01-01

This paper presents a computer tool called DSC (Simulation based Controllers Design) that enables an easy design of control systems and strategies applied to wastewater treatment plants. Although the control systems are developed and evaluated by simulation, this tool aims to facilitate the direct implementation of the designed control system to the PC of the full-scale WWTP (wastewater treatment plants). The designed control system can be programmed in a dedicated control application and can be connected to either the simulation software or the SCADA of the plant. To this end, the developed DSC incorporates an OPC server (OLE for process control) which facilitates an open-standard communication protocol for different industrial process applications. The potential capabilities of the DSC tool are illustrated through the example of a full-scale application. An aeration control system applied to a nutrient removing WWTP was designed, tuned and evaluated with the DSC tool before its implementation in the full scale plant. The control parameters obtained by simulation were suitable for the full scale plant with only few modifications to improve the control performance. With the DSC tool, the control systems performance can be easily evaluated by simulation. Once developed and tuned by simulation, the control systems can be directly applied to the full-scale WWTP.

Large- and small-scale environmental factors drive distributions of cool-adapted plants in karstic microrefugia

PubMed Central

Vojtkó, András; Farkas, Tünde; Szabó, Anna; Havadtői, Krisztina; Vojtkó, Anna E.; Tölgyesi, Csaba; Cseh, Viktória; Erdős, László; Maák, István Elek; Keppel, Gunnar

2017-01-01

Background and aims Dolines are small- to large-sized bowl-shaped depressions of karst surfaces. They may constitute important microrefugia, as thermal inversion often maintains cooler conditions within them. This study aimed to identify the effects of large- (macroclimate) and small-scale (slope aspect and vegetation type) environmental factors on cool-adapted plants in karst dolines of East-Central Europe. We also evaluated the potential of these dolines to be microrefugia that mitigate the effects of climate change on cool-adapted plants in both forest and grassland ecosystems. Methods We compared surveys of plant species composition that were made between 2007 and 2015 in 21 dolines distributed across four mountain ranges (sites) in Hungary and Romania. We examined the effects of environmental factors on the distribution and number of cool-adapted plants on three scales: (1) regional (all sites); (2) within sites and; (3) within dolines. Generalized linear models and non-parametric tests were used for the analyses. Key Results Macroclimate, vegetation type and aspect were all significant predictors of the diversity of cool-adapted plants. More cool-adapted plants were recorded in the coolest site, with only few found in the warmest site. At the warmest site, the distribution of cool-adapted plants was restricted to the deepest parts of dolines. Within sites of intermediate temperature and humidity, the effect of vegetation type and aspect on the diversity of cool-adapted plants was often significant, with more taxa being found in grasslands (versus forests) and on north-facing slopes (versus south-facing slopes). Conclusions There is large variation in the number and spatial distribution of cool-adapted plants in karst dolines, which is related to large- and small-scale environmental factors. Both macro- and microrefugia are therefore likely to play important roles in facilitating the persistence of cool-adapted plants under global warming. PMID:28025290

Method of installing a control room console in a nuclear power plant

DOEpatents

Scarola, Kenneth; Jamison, David S.; Manazir, Richard M.; Rescorl, Robert L.; Harmon, Daryl L.

1994-01-01

An advanced control room complex for a nuclear power plant, including a discrete indicator and alarm system (72) which is nuclear qualified for rapid response to changes in plant parameters and a component control system (64) which together provide a discrete monitoring and control capability at a panel (14-22, 26, 28) in the control room (10). A separate data processing system (70), which need not be nuclear qualified, provides integrated and overview information to the control room and to each panel, through CRTs (84) and a large, overhead integrated process status overview board (24). The discrete indicator and alarm system (72) and the data processing system (70) receive inputs from common plant sensors and validate the sensor outputs to arrive at a representative value of the parameter for use by the operator during both normal and accident conditions, thereby avoiding the need for him to assimilate data from each sensor individually. The integrated process status board (24) is at the apex of an information hierarchy that extends through four levels and provides access at each panel to the full display hierarchy. The control room panels are preferably of a modular construction, permitting the definition of inputs and outputs, the man machine interface, and the plant specific algorithms, to proceed in parallel with the fabrication of the panels, the installation of the equipment and the generic testing thereof.

Application of NASA-developed technology to the automatic control of municipal sewage treatment plants

NASA Technical Reports Server (NTRS)

Hiser, L. L.; Herrera, W. R.

1973-01-01

A search was made of NASA developed technology and commercial technology for process control sensors and instrumentation which would be applicable to the operation of municipal sewage treatment plants. Several notable items were found from which process control concepts were formulated that incorporated these items into systems to automatically operate municipal sewage treatment plants. A preliminary design of the most promising concept was developed into a process control scheme for an activated sludge treatment plant. This design included process control mechanisms for maintaining constant food to sludge mass (F/M) ratio, and for such unit processes as primary sedimentation, sludge wastage, and underflow control from the final clarifier.

Diversity, expansion, and evolutionary novelty of plant DNA-binding transcription factor families.

PubMed

Lehti-Shiu, Melissa D; Panchy, Nicholas; Wang, Peipei; Uygun, Sahra; Shiu, Shin-Han

2017-01-01

Plant transcription factors (TFs) that interact with specific sequences via DNA-binding domains are crucial for regulating transcriptional initiation and are fundamental to plant development and environmental response. In addition, expansion of TF families has allowed functional divergence of duplicate copies, which has contributed to novel, and in some cases adaptive, traits in plants. Thus, TFs are central to the generation of the diverse plant species that we see today. Major plant agronomic traits, including those relevant to domestication, have also frequently arisen through changes in TF coding sequence or expression patterns. Here our goal is to provide an overview of plant TF evolution by first comparing the diversity of DNA-binding domains and the sizes of these domain families in plants and other eukaryotes. Because TFs are among the most highly expanded gene families in plants, the birth and death process of TFs as well as the mechanisms contributing to their retention are discussed. We also provide recent examples of how TFs have contributed to novel traits that are important in plant evolution and in agriculture.This article is part of a Special Issue entitled: Plant Gene Regulatory Mechanisms and Networks, edited by Dr. Erich Grotewold and Dr. Nathan Springer. Copyright © 2016 Elsevier B.V. All rights reserved.

Risk factors of visceral leishmaniasis: a case control study in north-western Ethiopia.

PubMed

Yared, Solomon; Deribe, Kebede; Gebreselassie, Araya; Lemma, Wessenseged; Akililu, Essayas; Kirstein, Oscar D; Balkew, Meshesha; Warburg, Alon; Gebre-Michael, Teshome; Hailu, Asrat

2014-10-14

Visceral leishmaniasis (VL, also called ''kala-azar"), is a life threatening neglected tropical infectious disease which mainly affects the poorest of the poor. VL is prevalent in Ethiopia particularly in the northwest of the country. Understanding the risk factors of VL infection helps in its prevention and control. The aim of the present study was to identify the factors associated with VL. A case-control study was carried out during the period of January-July 2013 in northwest Ethiopia. Cases and controls were diagnosed using clinical presentation, the rk39 rapid diagnostic test and Direct Agglutination Test (DAT). A total of 283 (84.8% males versus 15.2% females) participants were interviewed. 90 cases and 193 controls were involved, matched by age, sex and geographical location with a ratio of 1:2 (case: controls). Univariate and backward multivariate conditional logistic regression were used to identify risk factors of VL. Elevated odds of VL was associated with goat ownership (OR = 6.4; 95%: confidence interval [Cl]: 1.5-28.4), living in houses with cracked wall (OR = 6.4; 95% Cl: 1.6-25.6), increased family size (OR = 1.3; 95% Cl: 1.0-1.8) and the number of days spent in the farm field (OR = 1.1; 95% Cl: 1.0-1.2). However, daily individual activities around the home and farm fields, mainly sleeping on a bed (OR = 0.2; 95%: Cl 0.03-0.9), sleeping outside the house under a bed net (OR = 0.1; 95% Cl: 0.02-0.36)] and smoking plant parts in the house during the night time (OR = 0.1; 95% Cl: 0.01-0.6) were associated with decreased odds of being VL case. Our findings showed that use of bed net and smoke could be helpful for the prevention of VL in the area particularly among individuals who spend most of their time in the farm. VL control effort could be focused on improving housing conditions, such as sealing cracks and crevices inside and outside houses. Further research is warranted to elucidate the role of goats in the transmission

Viral and Cellular Factors Involved in Phloem Transport of Plant Viruses

PubMed Central

Hipper, Clémence; Brault, Véronique; Ziegler-Graff, Véronique; Revers, Frédéric

2013-01-01

Phloem transport of plant viruses is an essential step in the setting-up of a complete infection of a host plant. After an initial replication step in the first cells, viruses spread from cell-to-cell through mesophyll cells, until they reach the vasculature where they rapidly move to distant sites in order to establish the infection of the whole plant. This last step is referred to as systemic transport, or long-distance movement, and involves virus crossings through several cellular barriers: bundle sheath, vascular parenchyma, and companion cells for virus loading into sieve elements (SE). Viruses are then passively transported within the source-to-sink flow of photoassimilates and are unloaded from SE into sink tissues. However, the molecular mechanisms governing virus long-distance movement are far from being understood. While most viruses seem to move systemically as virus particles, some viruses are transported in SE as viral ribonucleoprotein complexes (RNP). The nature of the cellular and viral factors constituting these RNPs is still poorly known. The topic of this review will mainly focus on the host and viral factors that facilitate or restrict virus long-distance movement. PMID:23745125

Controlling plant architecture by manipulation of gibberellic acid signalling in petunia

PubMed Central

Liang, Yin-Chih; Reid, Michael S; Jiang, Cai-Zhong

2014-01-01

Since stem elongation is a gibberellic acid (GA) response, GA inhibitors are commonly used to control plant height in the production of potted ornamentals and bedding plants. In this study, we investigated interfering with GA signaling by using molecular techniques as an alternative approach. We isolated three putative GID1 genes (PhGID1A, PhGID1B and PhGID1C) encoding GA receptors from petunia. Virus-induced gene silencing (VIGS) of these genes results in stunted growth, dark-green leaves and late-flowering. We also isolated the gai mutant gene (gai-1) from Arabidopsis. We have generated transgenic petunia plants in which the gai mutant protein is over-expressed under the control of a dexamethasone-inducible promoter. This system permits induction of the dominant Arabidopsis gai mutant gene at a desired stage of plant development in petunia plants by the application of dexamethasone (Dex). The induction of gai in Dex-treated T1 petunia seedlings caused dramatic growth retardation with short internodes. PMID:26504556

Baseline Evaluations to Support Control Room Modernization at Nuclear Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boring, Ronald L.; Joe, Jeffrey C.

2015-02-01

For any major control room modernization activity at a commercial nuclear power plant (NPP) in the U.S., a utility should carefully follow the four phases prescribed by the U.S. Nuclear Regulatory Commission in NUREG-0711, Human Factors Engineering Program Review Model. These four phases include Planning and Analysis, Design, Verification and Validation, and Implementation and Operation. While NUREG-0711 is a useful guideline, it is written primarily from the perspective of regulatory review, and it therefore does not provide a nuanced account of many of the steps the utility might undertake as part of control room modernization. The guideline is largely summative—intendedmore » to catalog final products—rather than formative—intended to guide the overall modernization process. In this paper, we highlight two crucial formative sub-elements of the Planning and Analysis phase specific to control room modernization that are not covered in NUREG-0711. These two sub-elements are the usability and ergonomics baseline evaluations. A baseline evaluation entails evaluating the system as-built and currently in use. The usability baseline evaluation provides key insights into operator performance using the control system currently in place. The ergonomics baseline evaluation identifies possible deficiencies in the physical configuration of the control system. Both baseline evaluations feed into the design of the replacement system and subsequent summative benchmarking activities that help ensure that control room modernization represents a successful evolution of the control system.« less

Agrochemical control of plant water use using engineered abscisic acid receptors.

PubMed

Park, Sang-Youl; Peterson, Francis C; Mosquna, Assaf; Yao, Jin; Volkman, Brian F; Cutler, Sean R

2015-04-23

Rising temperatures and lessening fresh water supplies are threatening agricultural productivity and have motivated efforts to improve plant water use and drought tolerance. During water deficit, plants produce elevated levels of abscisic acid (ABA), which improves water consumption and stress tolerance by controlling guard cell aperture and other protective responses. One attractive strategy for controlling water use is to develop compounds that activate ABA receptors, but agonists approved for use have yet to be developed. In principle, an engineered ABA receptor that can be activated by an existing agrochemical could achieve this goal. Here we describe a variant of the ABA receptor PYRABACTIN RESISTANCE 1 (PYR1) that possesses nanomolar sensitivity to the agrochemical mandipropamid and demonstrate its efficacy for controlling ABA responses and drought tolerance in transgenic plants. Furthermore, crystallographic studies provide a mechanistic basis for its activity and demonstrate the relative ease with which the PYR1 ligand-binding pocket can be altered to accommodate new ligands. Thus, we have successfully repurposed an agrochemical for a new application using receptor engineering. We anticipate that this strategy will be applied to other plant receptors and represents a new avenue for crop improvement.

Process control systems at Homer City coal preparation plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shell, W.P.

1983-03-01

An important part of process control engineering is the implementation of the basic control system design through commissioning to routine operation. This is a period when basic concepts can be reviewed and improvements either implemented or recorded for application in future systems. The experience of commissioning the process control systems in the Homer City coal cleaning plant are described and discussed. The current level of operating control performance in individual sections and the overall system are also reported and discussed.

Spectral composition of light and growing of plants in controlled environments

NASA Technical Reports Server (NTRS)

Tikhomirov, Alexander A.

1994-01-01

The main conclusions of many investigations about general requirements of plants for spectral composition of PAR (photosynthetically active radiation) are based on phylogenetic aspects of plant growth. We think that these aspects are not the main criteria in choosing the spectral composition required for growing plants in controlled conditions. Our approach to this problem is based on plant and crop reaction under long duration growth with specific spectra and intensity. Only in this way can we determine correctly the role of light characteristics for developing crops.

Plant Genome Complexity May Be a Factor Limiting In Situ the Transfer of Transgenic Plant Genes to the Phytopathogen Ralstonia solanacearum

PubMed Central

Bertolla, Franck; Pepin, Regis; Passelegue-Robe, Eugenie; Paget, Eric; Simkin, Andrew; Nesme, Xavier; Simonet, Pascal

2000-01-01

The development of natural competence by bacteria in situ is considered one of the main factors limiting transformation-mediated gene exchanges in the environment. Ralstonia solanacearum is a plant pathogen that is also a naturally transformable bacterium that can develop the competence state during infection of its host. We have attempted to determine whether this bacterium could become the recipient of plant genes. We initially demonstrated that plant DNA was released close to the infecting bacteria. We constructed and tested various combinations of transgenic plants and recipient bacteria to show that the effectiveness of such transfers was directly related to the ratio of the complexity of the plant genome to the number of copies of the transgene. PMID:10966449

Capsicum annuum homeobox 1 (CaHB1) is a nuclear factor that has roles in plant development, salt tolerance, and pathogen defense.

PubMed

Oh, Sang-Keun; Yoon, Joonseon; Choi, Gyung Ja; Jang, Hyun A; Kwon, Suk-Yoon; Choi, Doil

2013-12-06

Homeodomain-leucine zipper (HD-Zip) family proteins are unique to plants, but little is known about their role in defense responses. CaHB1 is a nuclear factor in peppers, belonging to subfamily II of HD-Zip proteins. Here, we determined the role of CaHB1 in the defense response. CaHB1 expression was induced when pepper plants were challenged with Phytophthora capsici, a plant pathogen to which peppers are susceptible, or environmental stresses such as drought and salt stimuli. CaHB1 was also highly expressed in pepper leaves following application of SA, whereas ethephon and MeJA had a moderate effect. To further investigate the function of CaHB1 in plants, we performed gain-of-function study by overexpression of CaHB1 in tomato. CaHB1-transgenic tomatoes showed significant growth enhancement including increased leaf thickness and enlarged cell size (1.8-fold larger than control plants). Microscopic analysis revealed that leaves from CaHB1-transgenic plants had thicker cell walls and cuticle layers than those from controls. Moreover, CaHB1-transgenic plants displayed enhanced resistance against Phytophthora infestans and increased tolerance to salt stress. Additionally, RT-PCR analysis of CaHB1-transgenic tomatoes revealed constitutive up-regulation of multiple genes involved in plant defense and osmotic stress. Therefore, our findings suggest roles for CaHB1 in development, salt stress, and pathogen defense. Copyright © 2013 Elsevier Inc. All rights reserved.

Adaptive Neural Network Algorithm for Power Control in Nuclear Power Plants

NASA Astrophysics Data System (ADS)

Masri Husam Fayiz, Al

2017-01-01

The aim of this paper is to design, test and evaluate a prototype of an adaptive neural network algorithm for the power controlling system of a nuclear power plant. The task of power control in nuclear reactors is one of the fundamental tasks in this field. Therefore, researches are constantly conducted to ameliorate the power reactor control process. Currently, in the Department of Automation in the National Research Nuclear University (NRNU) MEPhI, numerous studies are utilizing various methodologies of artificial intelligence (expert systems, neural networks, fuzzy systems and genetic algorithms) to enhance the performance, safety, efficiency and reliability of nuclear power plants. In particular, a study of an adaptive artificial intelligent power regulator in the control systems of nuclear power reactors is being undertaken to enhance performance and to minimize the output error of the Automatic Power Controller (APC) on the grounds of a multifunctional computer analyzer (simulator) of the Water-Water Energetic Reactor known as Vodo-Vodyanoi Energetichesky Reaktor (VVER) in Russian. In this paper, a block diagram of an adaptive reactor power controller was built on the basis of an intelligent control algorithm. When implementing intelligent neural network principles, it is possible to improve the quality and dynamic of any control system in accordance with the principles of adaptive control. It is common knowledge that an adaptive control system permits adjusting the controller’s parameters according to the transitions in the characteristics of the control object or external disturbances. In this project, it is demonstrated that the propitious options for an automatic power controller in nuclear power plants is a control system constructed on intelligent neural network algorithms.

Validation and implementation of model based control strategies at an industrial wastewater treatment plant.

PubMed

Demey, D; Vanderhaegen, B; Vanhooren, H; Liessens, J; Van Eyck, L; Hopkins, L; Vanrolleghem, P A

2001-01-01

In this paper, the practical implementation and validation of advanced control strategies, designed using model based techniques, at an industrial wastewater treatment plant is demonstrated. The plant under study is treating the wastewater of a large pharmaceutical production facility. The process characteristics of the wastewater treatment were quantified by means of tracer tests, intensive measurement campaigns and the use of on-line sensors. In parallel, a dynamical model of the complete wastewater plant was developed according to the specific kinetic characteristics of the sludge and the highly varying composition of the industrial wastewater. Based on real-time data and dynamic models, control strategies for the equalisation system, the polymer dosing and phosphorus addition were established. The control strategies are being integrated in the existing SCADA system combining traditional PLC technology with robust PC based control calculations. The use of intelligent control in wastewater treatment offers a wide spectrum of possibilities to upgrade existing plants, to increase the capacity of the plant and to eliminate peaks. This can result in a more stable and secure overall performance and, finally, in cost savings. The use of on-line sensors has a potential not only for monitoring concentrations, but also for manipulating flows and concentrations. This way the performance of the plant can be secured.

The plant cytoskeleton controls regulatory volume increase.

PubMed

Liu, Qiong; Qiao, Fei; Ismail, Ahmed; Chang, Xiaoli; Nick, Peter

2013-09-01

The ability to adjust cell volume is required for the adaptation to osmotic stress. Plant protoplasts can swell within seconds in response to hypoosmotic shock suggesting that membrane material is released from internal stores. Since the stability of plant membranes depends on submembraneous actin, we asked, whether this regulatory volume control depends on the cytoskeleton. As system we used two cell lines from grapevine which differ in their osmotic tolerance and observed that the cytoskeleton responded differently in these two cell lines. To quantify the ability for regulatory volume control, we used hydraulic conductivity (Lp) as readout and demonstrated a role of the cytoskeleton in protoplast swelling. Chelation of calcium, inhibition of calcium channels, or manipulation of membrane fluidity, did not significantly alter Lp, whereas direct manipulation of the cytoskeleton via specific chemical reagents, or indirectly, through the bacterial elicitor Harpin or activation of phospholipase D, was effective. By optochemical engineering of actin using a caged form of the phytohormone auxin we can break the symmetry of actin organisation resulting in a localised deformation of cell shape indicative of a locally increased Lp. We interpret our findings in terms of a model, where the submembraneous cytoskeleton controls the release of intracellular membrane stores during regulatory volume change. Copyright © 2013 Elsevier B.V. All rights reserved.

A cross-cultural study of organizational factors on safety: Japanese vs. Taiwanese oil refinery plants.

PubMed

Hsu, Shang Hwa; Lee, Chun-Chia; Wu, Muh-Cherng; Takano, Kenichi

2008-01-01

This study attempts to identify idiosyncrasies of organizational factors on safety and their influence mechanisms in Taiwan and Japan. Data were collected from employees of Taiwanese and Japanese oil refinery plants. Results show that organizational factors on safety differ in the two countries. Organizational characteristics in Taiwanese plants are highlighted as: higher level of management commitment to safety, harmonious interpersonal relationship, more emphasis on safety activities, higher devotion to supervision, and higher safety self-efficacy, as well as high quality of safety performance. Organizational characteristics in Japanese plants are highlighted as: higher level of employee empowerment and attitude towards continuous improvement, more emphasis on systematic safety management approach, efficient reporting system and teamwork, and high quality of safety performance. The casual relationships between organizational factors and workers' safety performance were investigated using structural equation modeling (SEM). Results indicate that the influence mechanisms of organizational factors in Taiwan and Japan are different. These findings provide insights into areas of safety improvement in emerging countries and developed countries respectively.

Epigenetic regulation of the expression of WRKY75 transcription factor in response to biotic and abiotic stresses in Solanaceae plants.

PubMed

López-Galiano, María José; González-Hernández, Ana I; Crespo-Salvador, Oscar; Rausell, Carolina; Real, M Dolores; Escamilla, Mónica; Camañes, Gemma; García-Agustín, Pilar; González-Bosch, Carmen; García-Robles, Inmaculada

2018-01-01

SlyWRKY75: gene expression was induced in response to biotic stresses, especially in Botrytis cinerea-infected tomato plants, in which Sly-miR1127-3p is a putative SlyWRKY75 regulator and epigenetic marks were detected. WRKY75 transcription factor involved in Pi homeostasis was recently found also induced in defense against necrotrophic pathogens. In this study, we analyzed by RT-qPCR the expression of SlyWRKY75 gene in tomato plants in response to abiotic stresses (drought or heat) and biotic stresses (Colorado potato beetle larvae infestation, Pseudomonas syringae or Botrytis cinerea infection) being only differentially expressed following biotic stresses, especially upon B. cinerea infection (55-fold induction). JA and JA-Ile levels were significantly increased in tomato plants under biotic stresses compared with control plants, indicating that SlyWRKY75 might be a transcriptional regulator of the JA pathway. The contribution of miRNAs and epigenetic molecular mechanisms to the regulation of this gene in B. cinerea-infected tomato plants was explored. We identified a putative Sly-miR1127-3p miRNA predicted to bind the intronic region of the SlyWRKY75 genomic sequence. Sly-miR1127-3p miRNA was repressed in infected plants (0.4-fold) supporting that it might act as an epigenetic regulation factor of SlyWRKY75 gene expression rather than via the post-transcriptional mechanisms of canonical miRNAs. It has been proposed that certain miRNAs can mediate DNA methylation in the plant nucleus broadening miRNA functions with transcriptional gene silencing by targeting intron-containing pre-mRNAs. Histone modifications analysis by chromatin immunoprecipitation (ChIP) demonstrated the presence of the activator histone modification H3K4me3 on SlyWRKY75 transcription start site and gene body. The induction of this gene in response to B. cinerea correlates with the presence of an activator mark. Thus, miRNAs and chromatin modifications might cooperate as epigenetic factors to

Soil-to-plant transfer factors of natural radionuclides (226Ra and 40K) in selected Thai medicinal plants.

PubMed

Saenboonruang, Kiadtisak; Phonchanthuek, Endu; Prasandee, Kamonkhuan

2018-04-01

A soil-to-plant transfer factor (TF) is an important parameter that could be used to estimate radionuclides levels in medicinal plants. This work reports concentrations of natural radionuclides ( 226 Ra and 40 K) and TFs in six Thai medicinal plants grown in central Thailand using an HPGe gamma ray spectrometer. Either root, leaf, or flower parts of each medicinal plant were selected for use in the investigation according to their practical uses in traditional medicine. The results showed that due to K being essential in plants, 40 K had higher arithmetic means of activity concentrations and geometric means of TFs (geometric standard deviations in parentheses) of 610 ± 260 Bq kg -1 dry weight (DW) and 2.0 (1.4), respectively, than 226 Ra, which had the activity concentrations and TFs of 4.8 ± 2.6 Bq kg -1 DW and 0.17 (1.8), respectively. The results also showed that the leaves of medicinal plants had higher activity concentrations and TFs than root and flower parts, probably due to higher metabolic activities in leaves. Furthermore, there was good agreement between the results from the current work and other similar reports on medicinal plants. The information obtained from this work could strengthen knowledge of natural radionuclides in plants and particularly increase available TF data on Thai medicinal plants. Copyright © 2018 Elsevier Ltd. All rights reserved.

33 CFR Appendix B to Part 273 - Information Requirements for Aquatic Plant Control Program Reports

Code of Federal Regulations, 2012 CFR

2012-07-01

... identification by common and scientific name of the plant or plants concerned, origin of infestation and likely... control operations or engineering works, including control methods, materials, equipment and procedures... operation control, the report should include a brief statement of the special problems in control methods...

33 CFR Appendix B to Part 273 - Information Requirements for Aquatic Plant Control Program Reports

Code of Federal Regulations, 2013 CFR

2013-07-01

... identification by common and scientific name of the plant or plants concerned, origin of infestation and likely... control operations or engineering works, including control methods, materials, equipment and procedures... operation control, the report should include a brief statement of the special problems in control methods...

RSL Class I Genes Controlled the Development of Epidermal Structures in the Common Ancestor of Land Plants.

PubMed

Proust, Hélène; Honkanen, Suvi; Jones, Victor A S; Morieri, Giulia; Prescott, Helen; Kelly, Steve; Ishizaki, Kimitsune; Kohchi, Takayuki; Dolan, Liam

2016-01-11

The colonization of the land by plants, sometime before 470 million years ago, was accompanied by the evolution tissue systems [1-3]. Specialized structures with diverse functions-from nutrient acquisition to reproduction-derived from single cells in the outermost layer (epidermis) were important sources of morphological innovation at this time [2, 4, 5]. In extant plants, these structures may be unicellular extensions, such as root hairs or rhizoids [6-9], or multicellular structures, such as asexual propagules or secretory hairs (papillae) [10-12]. Here, we show that a ROOTHAIR DEFECTIVE SIX-LIKE (RSL) class I basic helix-loop-helix transcription factor positively regulates the development of the unicellular and multicellular structures that develop from individual cells that expand out of the epidermal plane of the liverwort Marchantia polymorpha; mutants that lack MpRSL1 function do not develop rhizoids, slime papillae, mucilage papillae, or gemmae. Furthermore, we discovered that RSL class I genes are also required for the development of multicellular axillary hairs on the gametophyte of the moss Physcomitrella patens. Because class I RSL proteins also control the development of rhizoids in mosses and root hairs in angiosperms [13, 14], these data demonstrate that the function of RSL class I genes was to control the development of structures derived from single epidermal cells in the common ancestor of the land plants. Class I RSL genes therefore controlled the generation of adaptive morphological diversity as plants colonized the land from the water. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

RSL Class I Genes Controlled the Development of Epidermal Structures in the Common Ancestor of Land Plants

PubMed Central

Proust, Hélène; Honkanen, Suvi; Jones, Victor A.S.; Morieri, Giulia; Prescott, Helen; Kelly, Steve; Ishizaki, Kimitsune; Kohchi, Takayuki; Dolan, Liam

2016-01-01

Summary The colonization of the land by plants, sometime before 470 million years ago, was accompanied by the evolution tissue systems [1, 2, 3]. Specialized structures with diverse functions—from nutrient acquisition to reproduction—derived from single cells in the outermost layer (epidermis) were important sources of morphological innovation at this time [2, 4, 5]. In extant plants, these structures may be unicellular extensions, such as root hairs or rhizoids [6, 7, 8, 9], or multicellular structures, such as asexual propagules or secretory hairs (papillae) [10, 11, 12]. Here, we show that a ROOTHAIR DEFECTIVE SIX-LIKE (RSL) class I basic helix-loop-helix transcription factor positively regulates the development of the unicellular and multicellular structures that develop from individual cells that expand out of the epidermal plane of the liverwort Marchantia polymorpha; mutants that lack MpRSL1 function do not develop rhizoids, slime papillae, mucilage papillae, or gemmae. Furthermore, we discovered that RSL class I genes are also required for the development of multicellular axillary hairs on the gametophyte of the moss Physcomitrella patens. Because class I RSL proteins also control the development of rhizoids in mosses and root hairs in angiosperms [13, 14], these data demonstrate that the function of RSL class I genes was to control the development of structures derived from single epidermal cells in the common ancestor of the land plants. Class I RSL genes therefore controlled the generation of adaptive morphological diversity as plants colonized the land from the water. PMID:26725198

Controlled environment life support system: Calcium-related leaf injuries on plants

NASA Technical Reports Server (NTRS)

Tibbitts, T. W.

1986-01-01

Calcium related injuries to plants grown in controlled environments under conditions which maximize plant growth rates are described. Procedures to encourage movement of calcium into developing leaves of lettuce plants were investigated. The time course and pattern of calcium accumulation was determined to develop effective control procedures for this injury, termed tipburn. Procedures investigated were: (1) increasing the relative humidity to saturation during the dark period and altering root temperatures, (2) maximizing water stress during light and minimizing water stress during dark periods, (3) shortening the light-dark cycle lengths in combination with elevated moisture levels during the dark cycles, (4) reducing nutrient concentrations and (5) vibrating the plants. Saturated humidities at night increased the rate of growth and the large fluctuation in plant water potential encouraged calcium movement to the young leaves and delayed tipburn. Root temperature regulation between 15 and 26 C was not effective in preventing tipburn. Attempts to modulate water stress produced little variation, but no difference in tipburn development. Variations in light-dark cycle lengths also had no effect on calcium concentrations within developing leaves and no variation in tipburn development. Low concentrations of nutrient solution delayed tipburn, presumably because of greater calcium transport in the low concentration plants. Shaking of the plants did not prevent tipburn, but did delay it slightly.

Implementation of Autonomous Control Technology for Plant Growth Chambers

NASA Technical Reports Server (NTRS)

Costello, Thomas A.; Sager, John C.; Krumins, Valdis; Wheeler, Raymond M.

2002-01-01

The Kennedy Space Center has significant infrastructure for research using controlled environment plant growth chambers. Such research supports development of bioregenerative life support technology for long-term space missions. Most of the existing chambers in Hangar L and Little L will be moved to the new Space Experiment Research and Processing Laboratory (SERPL) in the summer of 2003. The impending move has created an opportunity to update the control system technologies to allow for greater flexibility, less labor for set-up and maintenance, better diagnostics, better reliability and easier data retrieval. Part of these improvements can be realized using hardware which communicates through an ethernet connection to a central computer for supervisory control but can be operated independently of the computer during routine run-time. Both the hardware and software functionality of an envisioned system were tested on a prototype plant growth chamber (CEC-4) in Hangar L. Based upon these tests, recommendations for hardware and software selection and system design for implementation in SERPL are included.

Genetic control of plant height in European winter wheat cultivars.

PubMed

Würschum, Tobias; Langer, Simon M; Longin, C Friedrich H

2015-05-01

Plant height variation in European winter wheat cultivars is mainly controlled by the Rht - D1 and Rht - B1 semi-dwarfing genes, but also by other medium- or small-effect QTL and potentially epistatic QTL enabling fine adjustments of plant height. Plant height is an important goal in wheat (Triticum aestivum L.) breeding as it affects crop performance and thus yield and quality. The aim of this study was to investigate the genetic control of plant height in European winter wheat cultivars. To this end, a panel of 410 winter wheat varieties from across Europe was evaluated for plant height in multi-location field trials and genotyped for the candidate loci Rht-B1, Rht-D1, Rht8, Ppd-B1 copy number variation and Ppd-D1 as well as by a genotyping-by-sequencing approach yielding 23,371 markers with known map position. We found that Rht-D1 and Rht-B1 had the largest effects on plant height in this cultivar collection explaining 40.9 and 15.5% of the genotypic variance, respectively, while Ppd-D1 and Rht8 accounted for 3.0 and 2.0% of the variance, respectively. A genome-wide scan for marker-trait associations yielded two additional medium-effect QTL located on chromosomes 6A and 5B explaining 11.0 and 5.7% of the genotypic variance after the effects of the candidate loci were accounted for. In addition, we identified several small-effect QTL as well as epistatic QTL contributing to the genetic architecture of plant height. Taken together, our results show that the two Rht-1 semi-dwarfing genes are the major sources of variation in European winter wheat cultivars and that other small- or medium-effect QTL and potentially epistatic QTL enable fine adjustments in plant height.

In vivo assessment of plant extracts for control of plant diseases: A sesquiterpene ketolactone isolated from Curcuma zedoaria suppresses wheat leaf rust.

PubMed

Han, Jae Woo; Shim, Sang Hee; Jang, Kyoung Soo; Choi, Yong Ho; Dang, Quang Le; Kim, Hun; Choi, Gyung Ja

2018-02-01

As an alternative to synthetic pesticides, natural materials such as plant extracts and microbes have been considered to control plant diseases. In this study, methanol extracts of 120 plants were explored for in vivo antifungal activity against Rhizoctonia solani, Botrytis cinerea, Phytophthora infestans, Puccinia triticina, and Blumeria graminis f. sp. hordei. Of the 120 plant extracts, eight plant extracts exhibited a disease control efficacy of more than 90% against at least one of five plant diseases. In particular, a methanol extract of Curcuma zedoaria rhizomes exhibited strong activity against wheat leaf rust caused by P. triticina. When the C. zedoaria methanol extracts were partitioned with various solvents, the layers of n-hexane, methylene chloride, and ethyl acetate showed disease control values of 100, 80, and 43%, respectively, against wheat leaf rust. From the C. zedoaria rhizome extracts, an antifungal substance was isolated and identified as a sesquiterpene ketolactone based on the mass and nuclear magnetic resonance spectral data. The active compound controlled the development of rice sheath blight, wheat leaf rust, and tomato late blight. Considering the in vivo antifungal activities of the sesquiterpene ketolactone and the C. zedoaria extracts, these results suggest that C. zedoaria can be used as a potent fungicide in organic agriculture.

Cell wall matrix polysaccharide distribution and cortical microtubule organization: two factors controlling mesophyll cell morphogenesis in land plants.

PubMed

Sotiriou, P; Giannoutsou, E; Panteris, E; Apostolakos, P; Galatis, B

2016-03-01

This work investigates the involvement of local differentiation of cell wall matrix polysaccharides and the role of microtubules in the morphogenesis of mesophyll cells (MCs) of three types (lobed, branched and palisade) in the dicotyledon Vigna sinensis and the fern Asplenium nidus. Homogalacturonan (HGA) epitopes recognized by the 2F4, JIM5 and JIM7 antibodies and callose were immunolocalized in hand-made leaf sections. Callose was also stained with aniline blue. We studied microtubule organization by tubulin immunofluorescence and transmission electron microscopy. In both plants, the matrix cell wall polysaccharide distribution underwent definite changes during MC differentiation. Callose constantly defined the sites of MC contacts. The 2F4 HGA epitope in V. sinensis first appeared in MC contacts but gradually moved towards the cell wall regions facing the intercellular spaces, while in A. nidus it was initially localized at the cell walls delimiting the intercellular spaces, but finally shifted to MC contacts. In V. sinensis, the JIM5 and JIM7 HGA epitopes initially marked the cell walls delimiting the intercellular spaces and gradually shifted in MC contacts, while in A. nidus they constantly enriched MC contacts. In all MC types examined, the cortical microtubules played a crucial role in their morphogenesis. In particular, in palisade MCs, cortical microtubule helices, by controlling cellulose microfibril orientation, forced these MCs to acquire a truncated cone-like shape. Unexpectedly in V. sinensis, the differentiation of colchicine-affected MCs deviated completely, since they developed a cell wall ingrowth labyrinth, becoming transfer-like cells. The results of this work and previous studies on Zea mays (Giannoutsou et al., Annals of Botany 2013; 112: : 1067-1081) revealed highly controlled local cell wall matrix differentiation in MCs of species belonging to different plant groups. This, in coordination with microtubule-dependent cellulose microfibril

Cell wall matrix polysaccharide distribution and cortical microtubule organization: two factors controlling mesophyll cell morphogenesis in land plants

PubMed Central

Sotiriou, P.; Giannoutsou, E.; Panteris, E.; Apostolakos, P.; Galatis, B.

2016-01-01

Background and aims This work investigates the involvement of local differentiation of cell wall matrix polysaccharides and the role of microtubules in the morphogenesis of mesophyll cells (MCs) of three types (lobed, branched and palisade) in the dicotyledon Vigna sinensis and the fern Asplenium nidus. Methods Homogalacturonan (HGA) epitopes recognized by the 2F4, JIM5 and JIM7 antibodies and callose were immunolocalized in hand-made leaf sections. Callose was also stained with aniline blue. We studied microtubule organization by tubulin immunofluorescence and transmission electron microscopy. Results In both plants, the matrix cell wall polysaccharide distribution underwent definite changes during MC differentiation. Callose constantly defined the sites of MC contacts. The 2F4 HGA epitope in V. sinensis first appeared in MC contacts but gradually moved towards the cell wall regions facing the intercellular spaces, while in A. nidus it was initially localized at the cell walls delimiting the intercellular spaces, but finally shifted to MC contacts. In V. sinensis, the JIM5 and JIM7 HGA epitopes initially marked the cell walls delimiting the intercellular spaces and gradually shifted in MC contacts, while in A. nidus they constantly enriched MC contacts. In all MC types examined, the cortical microtubules played a crucial role in their morphogenesis. In particular, in palisade MCs, cortical microtubule helices, by controlling cellulose microfibril orientation, forced these MCs to acquire a truncated cone-like shape. Unexpectedly in V. sinensis, the differentiation of colchicine-affected MCs deviated completely, since they developed a cell wall ingrowth labyrinth, becoming transfer-like cells. Conclusions The results of this work and previous studies on Zea mays (Giannoutsou et al., Annals of Botany 2013; 112: 1067–1081) revealed highly controlled local cell wall matrix differentiation in MCs of species belonging to different plant groups. This, in coordination

Pollen limitation in a narrow endemic plant: geographical variation and driving factors.

PubMed

Fernández, Juande D; Bosch, Jordi; Nieto-Ariza, Beatriz; Gómez, José M

2012-10-01

Pollen limitation may have important consequences for the reproduction and abundance of plant species. It may be especially harmful to endangered and endemic plants with small populations. In this study, we quantify the effect of pollen limitation on seed production and seedling emergence in an endangered narrow endemic crucifer, Erysimum popovii. We conducted a pollen addition experiment across the entire geographic distribution of the species, and explored the effect of pollinator assemblage, plant population size and density, and other habitat variables on pollen limitation intensity in 13 populations. We supplemented flowers in 20 plants per population with allogamous pollen. To account for potential resource reallocation, we used two types of control untreated flowers: internal control flowers from the same individual as the supplemented flowers, and external control flowers from other individuals. Our results indicate that E. popovii is pollen-limited in most of the populations studied, but only through seed production, since pollen supplementation did not enhance seedling emergence. Beefly abundance was associated with among-population differences in pollen limitation intensity. Populations in which beeflies were more abundant were less pollen-limited. In contrast, the abundance of other flower visitors, such as large bees or butterflies, was not associated with pollen limitation. Annual rainfall and bare soil cover were associated with the intensity of pollen limitation across populations.

Four Genes of Medicago truncatula Controlling Components of a Nod Factor Transduction Pathway

PubMed Central

Catoira, Romy; Galera, Christine; de Billy, Francoise; Penmetsa, R. Varma; Journet, Etienne-Pascal; Maillet, Fabienne; Rosenberg, Charles; Cook, Douglas; Gough, Clare; Dénarié, Jean

2000-01-01

Rhizobium nodulation (Nod) factors are lipo-chitooligosaccharides that act as symbiotic signals, eliciting several key developmental responses in the roots of legume hosts. Using nodulation-defective mutants of Medicago truncatula, we have started to dissect the genetic control of Nod factor transduction. Mutants in four genes (DMI1, DMI2, DMI3, and NSP) were pleiotropically affected in Nod factor responses, indicating that these genes are required for a Nod factor–activated signal transduction pathway that leads to symbiotic responses such as root hair deformations, expressions of nodulin genes, and cortical cell divisions. Mutant analysis also provides evidence that Nod factors have a dual effect on the growth of root hair: inhibition of endogenous (plant) tip growth, and elicitation of a novel tip growth dependent on (bacterial) Nod factors. dmi1, dmi2, and dmi3 mutants are also unable to establish a symbiotic association with endomycorrhizal fungi, indicating that there are at least three common steps to nodulation and endomycorrhization in M. truncatula and providing further evidence for a common signaling pathway between nodulation and mycorrhization. PMID:11006338

Control of Citrus Huanglongbing via Trunk Injection of Plant Defense Activators and Antibiotics.

PubMed

Hu, J; Jiang, J; Wang, N

2018-02-01

Citrus huanglongbing (HLB) or greening is a devastating disease of citrus worldwide and no effective control measure is currently available. Plant defense activators environmentally friendly compounds capable of inducing resistance against many plant pathogens. Earlier studies showed that foliar spray of plant defense inducers could slow down HLB disease progress. In this study, eight plant defense activators and three antibiotics were evaluated in three field trials for their effect to control HLB by trunk injection of young and mature sweet orange trees. Results showed that four trunk injections of several activators, including salicylic acid, oxalic acid, acibenzolar-S-methyl, and potassium phosphate, provided significant control of HLB by suppressing 'Candidatus Liberibacter asiaticus' titer and disease progress. Trunk injection of penicillin, streptomycin, and oxytetracycline hydrochloride resulted in excellent control of HLB. In general, antibiotics were more effective in reduction of 'Ca. L. asiaticus' titer and HLB symptom expressions than plant defense activators. These treatments also resulted in increased yield and better fruit quality. Injection of both salicylic acid and acibenzolar-S-methyl led to significant induction of pathogenesis-related (PR) genes PR-1 and PR-2 genes. Meanwhile, injection of either potassium phosphate or oxalic acid resulted in significant induction of PR-2 or PR-15 gene expression, respectively. These results suggested that HLB diseased trees remained inducible for systemic acquired resistance under field conditions. In summary, this study presents information regarding controlling HLB via trunk injection of plant defense activators and antibiotics, which helps citrus growers in decision making regarding developing an effective HLB management program.

Eleventh-year results of fertilization, herbaceous, and woody plant control in a loblolly pine plantation

Treesearch

James D. Haywood; Allan E. Tiarks

1990-01-01

Through 11 years, fertilization at planting significantly increased the stemwood volume (outside bark) per loblolly pine (Pinus taeda L.) on an intensively prepared moderately well-drained fine sandy loam site in northern Louisiana. Four years of herbaceous plant control significantly increased pine survival, and because herbaceous plant control...

Maximization of the annual energy production of wind power plants by optimization of layout and yaw-based wake control: Maximization of wind plant AEP by optimization of layout and wake control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gebraad, Pieter; Thomas, Jared J.; Ning, Andrew

This paper presents a wind plant modeling and optimization tool that enables the maximization of wind plant annual energy production (AEP) using yaw-based wake steering control and layout changes. The tool is an extension of a wake engineering model describing the steady-state effects of yaw on wake velocity profiles and power productions of wind turbines in a wind plant. To make predictions of a wind plant's AEP, necessary extensions of the original wake model include coupling it with a detailed rotor model and a control policy for turbine blade pitch and rotor speed. This enables the prediction of power productionmore » with wake effects throughout a range of wind speeds. We use the tool to perform an example optimization study on a wind plant based on the Princess Amalia Wind Park. In this case study, combined optimization of layout and wake steering control increases AEP by 5%. The power gains from wake steering control are highest for region 1.5 inflow wind speeds, and they continue to be present to some extent for the above-rated inflow wind speeds. The results show that layout optimization and wake steering are complementary because significant AEP improvements can be achieved with wake steering in a wind plant layout that is already optimized to reduce wake losses.« less

Mechanisms Controlling the Plant Diversity Effect on Soil Microbial Community Composition and Soil Microbial Diversity

NASA Astrophysics Data System (ADS)

Mellado Vázquez, P. G.; Lange, M.; Griffiths, R.; Malik, A.; Ravenek, J.; Strecker, T.; Eisenhauer, N.; Gleixner, G.

2015-12-01

Soil microorganisms are the main drivers of soil organic matter cycling. Organic matter input by living plants is the major energy and matter source for soil microorganisms, higher organic matter inputs are found in highly diverse plant communities. It is therefore relevant to understand how plant diversity alters the soil microbial community and soil organic matter. In a general sense, microbial biomass and microbial diversity increase with increasing plant diversity, however the mechanisms driving these interactions are not fully explored. Working with soils from a long-term biodiversity experiment (The Jena Experiment), we investigated how changes in the soil microbial dynamics related to plant diversity were explained by biotic and abiotic factors. Microbial biomass quantification and differentiation of bacterial and fungal groups was done by phospholipid fatty acid (PLFA) analysis; terminal-restriction fragment length polymorphism was used to determine the bacterial diversity. Gram negative (G-) bacteria predominated in high plant diversity; Gram positive (G+) bacteria were more abundant in low plant diversity and saprotrophic fungi were independent from plant diversity. The separation between G- and G+ bacteria in relation to plant diversity was governed by a difference in carbon-input related factors (e.g. root biomass and soil moisture) between plant diversity levels. Moreover, the bacterial diversity increased with plant diversity and the evenness of the PLFA markers decreased. Our results showed that higher plant diversity favors carbon-input related factors and this in turn favors the development of microbial communities specialized in utilizing new carbon inputs (i.e. G- bacteria), which are contributing to the export of new C from plants to soils.

The bHLH transcription factor BIS1 controls the iridoid branch of the monoterpenoid indole alkaloid pathway in Catharanthus roseus

PubMed Central

Van Moerkercke, Alex; Steensma, Priscille; Schweizer, Fabian; Pollier, Jacob; Gariboldi, Ivo; Payne, Richard; Vanden Bossche, Robin; Miettinen, Karel; Espoz, Javiera; Purnama, Purin Candra; Kellner, Franziska; Seppänen-Laakso, Tuulikki; O’Connor, Sarah E.; Rischer, Heiko; Memelink, Johan; Goossens, Alain

2015-01-01

Plants make specialized bioactive metabolites to defend themselves against attackers. The conserved control mechanisms are based on transcriptional activation of the respective plant species-specific biosynthetic pathways by the phytohormone jasmonate. Knowledge of the transcription factors involved, particularly in terpenoid biosynthesis, remains fragmentary. By transcriptome analysis and functional screens in the medicinal plant Catharanthus roseus (Madagascar periwinkle), the unique source of the monoterpenoid indole alkaloid (MIA)-type anticancer drugs vincristine and vinblastine, we identified a jasmonate-regulated basic helix–loop–helix (bHLH) transcription factor from clade IVa inducing the monoterpenoid branch of the MIA pathway. The bHLH iridoid synthesis 1 (BIS1) transcription factor transactivated the expression of all of the genes encoding the enzymes that catalyze the sequential conversion of the ubiquitous terpenoid precursor geranyl diphosphate to the iridoid loganic acid. BIS1 acted in a complementary manner to the previously characterized ethylene response factor Octadecanoid derivative-Responsive Catharanthus APETALA2-domain 3 (ORCA3) that transactivates the expression of several genes encoding the enzymes catalyzing the conversion of loganic acid to the downstream MIAs. In contrast to ORCA3, overexpression of BIS1 was sufficient to boost production of high-value iridoids and MIAs in C. roseus suspension cell cultures. Hence, BIS1 might be a metabolic engineering tool to produce sustainably high-value MIAs in C. roseus plants or cultures. PMID:26080427

Salinity controls on plant transpiration and soil water balance

NASA Astrophysics Data System (ADS)

Perri, S.; Molini, A.; Suweis, S. S.; Viola, F.; Entekhabi, D.

2017-12-01

Soil salinization and aridification represent a major threat for the food security and sustainable development of drylands. The two problems are deeply connected, and their interplay is expected to be further enhanced by climate change and projected population growth. Salt-affected land is currently estimated to cover around 1.1 Gha, and is particularly widespread in semi-arid to hyper-arid climates. Over 900 Mha of these saline/sodic soils are potentially available for crop or biomass production. Salt-tolerant plants have been recently proposed as valid solution to exploit or even remediate salinized soils. However the effects of salinity on evapotranspiration, soil water balance and the long-term salt mass balance in the soil, are still largely unexplored. In this contribution we analyze the feedback of evapotranspiration on soil salinization, with particular emphasis on the role of vegetation and plant salt-tolerance. The goal is to introduce a simple modeling framework able to shed some light on how (a) soil salinity controls plant transpiration, and (b) salinization itself is favored/impeded by different vegetation feedback. We introduce at this goal a spatially lumped stochastic model of soil moisture and salt mass dynamics averaged over the active soil depth, and accounting for the effect of salinity on evapotranspiration. Here, the limiting effect of salinity on ET is modeled through a simple plant response function depending on both salt concentration in the soil and plant salt-tolerance. The coupled soil moisture and salt mass balance is hence used to obtain the conditional steady-state probability density function (pdf) of soil moisture for given salt tolerance and salinization level, Our results show that salinity imposes a limit in the soil water balance and this limit depends on plant salt-tolerance mainly through the control of the leaching occurrence (tolerant plants exploit water more efficiently than the sensitive ones). We also analyzed the

Using Plant Phylogenetic Relatedness as a Predictor for Plants' Control on Soil Microbial Communities and Nitrogen Cycling

NASA Astrophysics Data System (ADS)

Potter, T.; Bowman, W. D.

2016-12-01

Despite the known importance of soil microbes and their influence on soil processes, a mechanistic understanding is still needed to predict how plants and soil microbes interact at scales that are relevant to community and ecosystem-scale processes. Closely related plant species have similar traits aboveground, but we don't know whether this is also true for belowground traits that affect soil microbial community structure and function. Determining how tightly plant phylogeny and plant functional traits are linked to soil microbial communities is a useful approach for discovering plant-microbe associations that are generalizable across plant species (a limitation of studies that employ a single or few plant species). Using this approach, we conducted a greenhouse study with seven congeneric grasses (genus Poa) and their native soils to examine whether plants' influences on microbial community structure were consistent with plant phylogenetic relatedness and/or plant functional traits. Seeds of each Poa species were planted in native soil (from the seed source population) as well as a homogenized soil from all seven populations. Additionally, a nitrogen treatment was added to address how an environmental change (such as nitrogen deposition) alters plant-microbe associations. Rhizosphere community composition of bacteria and fungi was obtained via marker gene sequencing to compare community composition across plant species. Patterns in plant-microbe associations across plant species reveal plant control on nutrient cycling via plant species' influence on microbial community structure. These results determine if we are ready to generalize about plant-microbe interactions at the genus level, an important stepping-stone to applying knowledge of plant-microbe interactions to larger ecological scales.

Spot drip application of dimethyl disulfide as a post-plant treatment for the control of plant parasitic nematodes and soilborne pathogens in grape production.

PubMed

Cabrera, J Alfonso; Wang, Dong; Gerik, James S; Gan, Jay

2014-07-01

Plant parasitic nematodes and soilborne pathogens can reduce the overall productivity in grape production. Not all grape growers apply soil fumigants before planting, and there is no single rootstock resistant to all nematode species. The aim of this investigation was to evaluate the effect of dimethyl disulfide (DMDS) applied at 112, 224, 448 and 897 kg ha(-1) as a post-plant treatment against soilborne plant parasitic nematodes and pathogens on the grape yield in established grapevines. In microplot and field trials, post-plant fumigation with DMDS controlled citrus (Tylenchulus semipenetrans), root-knot (Meloidogyne spp.), pin (Paratylenchus spp.) and ring (Mesocriconema xenoplax) nematodes in established Thomson Seedless grapevines. However, DMDS did not control the soilborne pathogens Pythium ultimum and Fusarium oxysporum. No indications of phytotoxicity were detected after post-plant fumigation with DMDS. In the field trial, grape yield was significantly higher with the lowest DMDS rate, but no difference among other rates was observed in comparison with the untreated control. Post-plant fumigation with DMDS controlled plant parasitic nematodes in established grapevines but was less efficacious against soilborne pathogens. Low rates of DMDS were sufficient for nematode control and increased the grape yield, probably without affecting beneficial soil organisms. Further research on evaluating the potential effect of DMDS against beneficial soil organisms is needed. Published 2013. This article is a U.S. Government work and is in the public domain in the USA.

Soft Sensors: Chemoinformatic Model for Efficient Control and Operation in Chemical Plants.

PubMed

Funatsu, Kimito

2016-12-01

Soft sensor is statistical model as an essential tool for controlling pharmaceutical, chemical and industrial plants. I introduce soft sensor, the roles, the applications, the problems and the research examples such as adaptive soft sensor, database monitoring and efficient process control. The use of soft sensor enables chemical industrial plants to be operated more effectively and stably. © 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Poultry Plant Noise Control

NASA Astrophysics Data System (ADS)

1982-01-01

A demonstration conducted last winter at the Tip Top Poultry Plant intended to show poultry plant managers from all over the U.S. potential solutions to the problem of plant noise. Plastic covers used over sound absorbing materials need to meet cleanability requirements, high- pressure water cleaning and other harsh maintenance procedures peculiar to the poultry processing industry. For the demonstration, Fiber Flex, Inc. manufactured and donated 750 noise panels; Owens-Corning Fiberglas Corporation donated the fiberglas cores; and the cover material was purchased from Howe and Bainbridge. The Engineering Experiment Station (EES) conducted before and after noise surveys and is evaluating the effect of noise reduction on turnover and productivity in the demonstration plant. EES plans to conduct a noise abatement workshop and update a handbook to help poultry processors with noise problems. EES study and demonstration may be applicable to other food processing plants where similar sanitary constraints exist.

Major controlling factors and prediction models for arsenic uptake from soil to wheat plants.

PubMed

Dai, Yunchao; Lv, Jialong; Liu, Ke; Zhao, Xiaoyan; Cao, Yingfei

2016-08-01

The application of current Chinese agriculture soil quality standards fails to evaluate the land utilization functions appropriately due to the diversity of soil properties and plant species. Therefore, the standards should be amended. A greenhouse experiment was conducted to investigate arsenic (As) enrichment in various soils from 18 Chinese provinces in parallel with As transfer to 8 wheat varieties. The goal of the study was to build and calibrate soil-wheat threshold models to forecast the As threshold of wheat soils. In Shaanxi soils, Wanmai and Jimai were the most sensitive and insensitive wheat varieties, respectively; and in Jiangxi soils, Zhengmai and Xumai were the most sensitive and insensitive wheat varieties, respectively. Relationships between soil properties and the bioconcentration factor (BCF) were built based on stepwise multiple linear regressions. Soil pH was the best predictor of BCF, and after normalizing the regression equation (Log BCF=0.2054 pH- 3.2055, R(2)=0.8474, n=14, p<0.001), we obtained a calibrated model. Using the calibrated model, a continuous soil-wheat threshold equation (HC5=10((-0.2054 pH+2.9935))+9.2) was obtained for the species-sensitive distribution curve, which was built on Chinese food safety standards. The threshold equation is a helpful tool that can be applied to estimate As uptake from soil to wheat. Copyright © 2016 Elsevier Inc. All rights reserved.

Plant Abiotic Stress Proteomics: The Major Factors Determining Alterations in Cellular Proteome

PubMed Central

Kosová, Klára; Vítámvás, Pavel; Urban, Milan O.; Prášil, Ilja T.; Renaut, Jenny

2018-01-01

HIGHLIGHTS: Major environmental and genetic factors determining stress-related protein abundance are discussed.Major aspects of protein biological function including protein isoforms and PTMs, cellular localization and protein interactions are discussed.Functional diversity of protein isoforms and PTMs is discussed. Abiotic stresses reveal profound impacts on plant proteomes including alterations in protein relative abundance, cellular localization, post-transcriptional and post-translational modifications (PTMs), protein interactions with other protein partners, and, finally, protein biological functions. The main aim of the present review is to discuss the major factors determining stress-related protein accumulation and their final biological functions. A dynamics of stress response including stress acclimation to altered ambient conditions and recovery after the stress treatment is discussed. The results of proteomic studies aimed at a comparison of stress response in plant genotypes differing in stress adaptability reveal constitutively enhanced levels of several stress-related proteins (protective proteins, chaperones, ROS scavenging- and detoxification-related enzymes) in the tolerant genotypes with respect to the susceptible ones. Tolerant genotypes can efficiently adjust energy metabolism to enhanced needs during stress acclimation. Stress tolerance vs. stress susceptibility are relative terms which can reflect different stress-coping strategies depending on the given stress treatment. The role of differential protein isoforms and PTMs with respect to their biological functions in different physiological constraints (cellular compartments and interacting partners) is discussed. The importance of protein functional studies following high-throughput proteome analyses is presented in a broader context of plant biology. In summary, the manuscript tries to provide an overview of the major factors which have to be considered when interpreting data from proteomic

Pathogen filtration to control plant disease outbreak in greenhouse production

NASA Astrophysics Data System (ADS)

Jeon, Sangho; Krasnow, Charles; Bhalsod, Gemini; Granke, Leah; Harlan, Blair; Hausbeck, Mary; Zhang, Wei

2016-04-01

Previous research has been extensively focused on understanding the fate and transport of human microbial pathogens in soil and water environments. However, little is known about the transport of plant pathogens, although these pathogens are often found in irrigation waters and could cause severe crop damage and economical loss. Water mold pathogens including Phytophthora spp. and Pythium spp. are infective to a wide range of vegetable and floriculture crops, and they are primarily harbored in soils and disseminated through water flow. It is challenging to control these pathogens because they often quickly develop resistance to many fungicides. Therefore, this multi-scale study aimed to investigate physical removal of plant pathogens from water by filtration, thus reducing the pathogen exposure risks to crops. In column-scale experiments, we studied controlling factors on the transport and retention of Phytophthora capsici zoospores in saturated columns packed with iron oxide coated-sand and uncoated-sand under varying solution chemistry. Biflagellate zoospores were less retained than encysted zoospores, and lower solution pH and greater iron oxide content increased the retention of encysted zoospores. These results provided insights on environmental dispersal of Phytophthora zoospores in natural soils as well as on developing cost-effective engineered filtration systems for pathogen removal. Using small-scale greenhouse filtration systems, we further investigated the performance of varying filter media (i.e., granular sand, iron oxide coated ceramic porous media, and activated carbon) in mitigating disease outbreaks of Phytophthora and Pythium for greenhouse-grown squash and poinsettia, respectively, in comparison with fungicide treatment. For squash, filtration by iron oxide coated media was more effective in reducing the Phytophthora infection, comparing to sand filtration and fungicide application. For poinsettia, sand filtration performed better in controlling

Within-plant distribution of Aulacorthum solani (Hemiptera: Aphididae), on various greenhouse plants with implications for control.

PubMed

Jandricic, S E; Mattson, N S; Wraight, S P; Sanderson, J P

2014-04-01

Foxglove aphid, Aulacorthum solani (Kaltenbach) (Hemiptera: Aphididae), has recently undergone a status change from an occasional pest to a serious pest in greenhouses of North America and the United Kingdom. Little nonanecdotal information exists on the ecology of this insect in greenhouse crops. To help improve integrated pest management decisions for A. solani, the within-plant distribution of this pest was explored on a variety of common greenhouse plants in both the vegetative and flowering stage. This aphid generally was found on lower leaves of vegetative plants, but was found higher in the canopy on reproductive plants (on flowers, flower buds, or upper leaves). Aphid numbers were not consistently positively correlated with total leaf surface areas within plant strata across plant species. Thus, the observed differences in preferred feeding sites on vegetative versus flowering plants are possibly a response to differences in nutritional quality of the various host-plant tissues. Despite being anecdotally described as a "stem-feeding aphid," A. solani was rarely found feeding on stems at the population densities established in our tests, with the exception of racemes of scarlet sage (Salvia splendans). Although some previous reports suggested that A. solani prefers to feed on new growth of plants, our results indicate that mature leaves are preferred over growing tips and young leaves. The implications of the within-plant feeding preferences of A. solani populations with respect to both biological and chemical control are discussed.

A Vitis vinifera basic helix-loop-helix transcription factor enhances plant cell size, vegetative biomass and reproductive yield

DOE PAGES

Lim, Sung Don; Yim, Won Choel; Liu, Degao; ...

2018-04-16

Strategies for improving plant size are critical targets for plant biotechnology to increase vegetative biomass or reproductive yield. To improve biomass production, a codon-optimized helix–loop–helix transcription factor (VvCEB1 opt) from wine grape was overexpressed in Arabidopsis thaliana resulting in significantly increased leaf number, leaf and rosette area, fresh weight and dry weight. Cell size, but typically not cell number, was increased in all tissues resulting in increased vegetative biomass and reproductive organ size, number and seed yield. Ionomic analysis of leaves revealed the VvCEB1 opt-overexpressing plants had significantly elevated, K, S and Mo contents relative to control lines. Increased Kmore » content likely drives increased osmotic potential within cells leading to greater cellular growth and expansion. To understand the mechanistic basis of VvCEB1 opt action, one transgenic line was genotyped using RNA-Seq mRNA expression profiling and revealed a novel transcriptional reprogramming network with significant changes in mRNA abundance for genes with functions in delayed flowering, pathogen–defence responses, iron homeostasis, vesicle-mediated cell wall formation and auxin-mediated signalling and responses. Direct testing of VvCEB1 opt-overexpressing plants showed that they had significantly elevated auxin content and a significantly increased number of lateral leaf primordia within meristems relative to controls, confirming that cell expansion and organ number proliferation were likely an auxin-mediated process. VvCEB1 opt overexpression in Nicotiana sylvestris also showed larger cells, organ size and biomass demonstrating the potential applicability of this innovative strategy for improving plant biomass and reproductive yield in crops.« less

A Vitis vinifera basic helix-loop-helix transcription factor enhances plant cell size, vegetative biomass and reproductive yield

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lim, Sung Don; Yim, Won Choel; Liu, Degao

Strategies for improving plant size are critical targets for plant biotechnology to increase vegetative biomass or reproductive yield. To improve biomass production, a codon-optimized helix–loop–helix transcription factor (VvCEB1 opt) from wine grape was overexpressed in Arabidopsis thaliana resulting in significantly increased leaf number, leaf and rosette area, fresh weight and dry weight. Cell size, but typically not cell number, was increased in all tissues resulting in increased vegetative biomass and reproductive organ size, number and seed yield. Ionomic analysis of leaves revealed the VvCEB1 opt-overexpressing plants had significantly elevated, K, S and Mo contents relative to control lines. Increased Kmore » content likely drives increased osmotic potential within cells leading to greater cellular growth and expansion. To understand the mechanistic basis of VvCEB1 opt action, one transgenic line was genotyped using RNA-Seq mRNA expression profiling and revealed a novel transcriptional reprogramming network with significant changes in mRNA abundance for genes with functions in delayed flowering, pathogen–defence responses, iron homeostasis, vesicle-mediated cell wall formation and auxin-mediated signalling and responses. Direct testing of VvCEB1 opt-overexpressing plants showed that they had significantly elevated auxin content and a significantly increased number of lateral leaf primordia within meristems relative to controls, confirming that cell expansion and organ number proliferation were likely an auxin-mediated process. VvCEB1 opt overexpression in Nicotiana sylvestris also showed larger cells, organ size and biomass demonstrating the potential applicability of this innovative strategy for improving plant biomass and reproductive yield in crops.« less

An In vitro Study of Bio-Control and Plant Growth Promotion Potential of Salicaceae Endophytes.

PubMed

Kandel, Shyam L; Firrincieli, Andrea; Joubert, Pierre M; Okubara, Patricia A; Leston, Natalie D; McGeorge, Kendra M; Mugnozza, Giuseppe S; Harfouche, Antoine; Kim, Soo-Hyung; Doty, Sharon L

2017-01-01

Microbial communities in the endosphere of Salicaceae plants, poplar ( Populus trichocarpa ) and willow ( Salix sitchensis ), have been demonstrated to be important for plant growth promotion, protection from biotic and abiotic stresses, and degradation of toxic compounds. Our study aimed to investigate bio-control activities of Salicaceae endophytes against various soil borne plant pathogens including Rhizoctonia solani AG-8, Fusarium culmorum, Gaeumannomyces graminis var. tritici , and Pythium ultimum . Additionally, different plant growth promoting traits such as biological nitrogen fixation (BNF), indole-3-acetic acid (IAA) biosynthesis, phosphate solubilization, and siderophore production were assessed in all bio-control positive strains. Burkholderia, Rahnella, Pseudomonas , and Curtobacterium were major endophyte genera that showed bio-control activities in the in-vitro assays. The bio-control activities of Burkholderia strains were stronger across all tested plant pathogens as compared to other stains. Genomes of sequenced Burkholderia strains WP40 and WP42 were surveyed to identify the putative genes involved in the bio-control activities. The ocf and hcnABC gene clusters responsible for biosynthesis of the anti-fungal metabolites, occidiofungin and hydrogen cyanide, are present in the genomes of WP40 and WP42. Nearly all endophyte strains showing the bio-control activities produced IAA, solubilized tricalcium phosphate, and synthesized siderophores in the culture medium. Moreover, some strains reduced acetylene into ethylene in the acetylene reduction assay, a common assay used for BNF. Salicaceae endophytes could be useful for bio-control of various plant pathogens, and plant growth promotion possibly through the mechanisms of BNF, IAA production, and nutrient acquisition.

Optimization of Photosynthetic Productivity in Contrasting Environments by Regulons Controlling Plant Form and Function

PubMed Central

Demmig-Adams, Barbara; Baker, Christopher R.

2018-01-01

We review the role of a family of transcription factors and their regulons in maintaining high photosynthetic performance across a range of challenging environments with a focus on extreme temperatures and water availability. Specifically, these transcription factors include CBFs (C-repeat binding factors) and DREBs (dehydration-responsive element-binding), with CBF/DREB1 primarily orchestrating cold adaptation and other DREBs serving in heat, drought, and salinity adaptation. The central role of these modulators in plant performance under challenging environments is based on (i) interweaving of these regulators with other key signaling networks (plant hormones and redox signals) as well as (ii) their function in integrating responses across the whole plant, from light-harvesting and sugar-production in the leaf to foliar sugar export and water import and on to the plant’s sugar-consuming sinks (growth, storage, and reproduction). The example of Arabidopsis thaliana ecotypes from geographic origins with contrasting climates is used to describe the links between natural genetic variation in CBF transcription factors and the differential acclimation of plant anatomical and functional features needed to support superior photosynthetic performance in contrasting environments. Emphasis is placed on considering different temperature environments (hot versus cold) and light environments (limiting versus high light), on trade-offs between adaptations to contrasting environments, and on plant lines minimizing such trade-offs. PMID:29543762

Large- and small-scale environmental factors drive distributions of cool-adapted plants in karstic microrefugia.

PubMed

Bátori, Zoltán; Vojtkó, András; Farkas, Tünde; Szabó, Anna; Havadtői, Krisztina; Vojtkó, Anna E; Tölgyesi, Csaba; Cseh, Viktória; Erdős, László; Maák, István Elek; Keppel, Gunnar

2017-01-01

Dolines are small- to large-sized bowl-shaped depressions of karst surfaces. They may constitute important microrefugia, as thermal inversion often maintains cooler conditions within them. This study aimed to identify the effects of large- (macroclimate) and small-scale (slope aspect and vegetation type) environmental factors on cool-adapted plants in karst dolines of East-Central Europe. We also evaluated the potential of these dolines to be microrefugia that mitigate the effects of climate change on cool-adapted plants in both forest and grassland ecosystems. We compared surveys of plant species composition that were made between 2007 and 2015 in 21 dolines distributed across four mountain ranges (sites) in Hungary and Romania. We examined the effects of environmental factors on the distribution and number of cool-adapted plants on three scales: (1) regional (all sites); (2) within sites and; (3) within dolines. Generalized linear models and non-parametric tests were used for the analyses. Macroclimate, vegetation type and aspect were all significant predictors of the diversity of cool-adapted plants. More cool-adapted plants were recorded in the coolest site, with only few found in the warmest site. At the warmest site, the distribution of cool-adapted plants was restricted to the deepest parts of dolines. Within sites of intermediate temperature and humidity, the effect of vegetation type and aspect on the diversity of cool-adapted plants was often significant, with more taxa being found in grasslands (versus forests) and on north-facing slopes (versus south-facing slopes). There is large variation in the number and spatial distribution of cool-adapted plants in karst dolines, which is related to large- and small-scale environmental factors. Both macro- and microrefugia are therefore likely to play important roles in facilitating the persistence of cool-adapted plants under global warming. © The Author 2016. Published by Oxford University Press on behalf of

Redox Modulation of Plant Developmental Regulators from the Class I TCP Transcription Factor Family1[W][OA

PubMed Central

Viola, Ivana L.; Güttlein, Leandro N.; Gonzalez, Daniel H.

2013-01-01

TEOSINTE BRANCHED1-CYCLOIDEA-PROLIFERATING CELL FACTOR1 (TCP) transcription factors participate in plant developmental processes associated with cell proliferation and growth. Most members of class I, one of the two classes that compose the family, have a conserved cysteine at position 20 (Cys-20) of the TCP DNA-binding and dimerization domain. We show that Arabidopsis (Arabidopsis thaliana) class I proteins with Cys-20 are sensitive to redox conditions, since their DNA-binding activity is inhibited after incubation with the oxidants diamide, oxidized glutathione, or hydrogen peroxide or with nitric oxide-producing agents. Inhibition can be reversed by treatment with the reductants dithiothreitol or reduced glutathione or by incubation with the thioredoxin/thioredoxin reductase system. Mutation of Cys-20 in the class I protein TCP15 abolished its redox sensitivity. Under oxidizing conditions, covalently linked dimers were formed, suggesting that inactivation is associated with the formation of intermolecular disulfide bonds. Inhibition of class I TCP protein activity was also observed in vivo, in yeast (Saccharomyces cerevisiae) cells expressing TCP proteins and in plants after treatment with redox agents. This inhibition was correlated with modifications in the expression of the downstream CUC1 gene in plants. Modeling studies indicated that Cys-20 is located at the dimer interface near the DNA-binding surface. This places this residue in the correct orientation for intermolecular disulfide bond formation and explains the sensitivity of DNA binding to the oxidation of Cys-20. The redox properties of Cys-20 and the observed effects of cellular redox agents both in vitro and in vivo suggest that class I TCP protein action is under redox control in plants. PMID:23686421

An approach to design controllers for MIMO fractional-order plants based on parameter optimization algorithm.

PubMed

Xue, Dingyü; Li, Tingxue

2017-04-27

The parameter optimization method for multivariable systems is extended to the controller design problems for multiple input multiple output (MIMO) square fractional-order plants. The algorithm can be applied to search for the optimal parameters of integer-order controllers for fractional-order plants with or without time delays. Two examples are given to present the controller design procedures for MIMO fractional-order systems. Simulation studies show that the integer-order controllers designed are robust to plant gain variations. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

Literature Review and Assessment of Plant and Animal Transfer Factors Used in Performance Assessment Modeling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robertson, David E.; Cataldo, Dominic A.; Napier, Bruce A.

2003-07-20

A literature review and assessment was conducted by Pacific Northwest National Laboratory (PNNL) to update information on plant and animal radionuclide transfer factors used in performance-assessment modeling. A group of 15 radionuclides was included in this review and assessment. The review is composed of four main sections, not including the Introduction. Section 2.0 provides a review of the critically important issue of physicochemical speciation and geochemistry of the radionuclides in natural soil-water systems as it relates to the bioavailability of the radionuclides. Section 3.0 provides an updated review of the parameters of importance in the uptake of radionuclides by plants,more » including root uptake via the soil-groundwater system and foliar uptake due to overhead irrigation. Section 3.0 also provides a compilation of concentration ratios (CRs) for soil-to-plant uptake for the 15 selected radionuclides. Section 4.0 provides an updated review on radionuclide uptake data for animal products related to absorption, homeostatic control, approach to equilibration, chemical and physical form, diet, and age. Compiled transfer coefficients are provided for cow’s milk, sheep’s milk, goat’s milk, beef, goat meat, pork, poultry, and eggs. Section 5.0 discusses the use of transfer coefficients in soil, plant, and animal modeling using regulatory models for evaluating radioactive waste disposal or decommissioned sites. Each section makes specific suggestions for future research in its area.« less

Evaluating the Spatio-Temporal Factors that Structure Network Parameters of Plant-Herbivore Interactions

PubMed Central

López-Carretero, Antonio; Díaz-Castelazo, Cecilia; Boege, Karina; Rico-Gray, Víctor

2014-01-01

Despite the dynamic nature of ecological interactions, most studies on species networks offer static representations of their structure, constraining our understanding of the ecological mechanisms involved in their spatio-temporal stability. This is the first study to evaluate plant-herbivore interaction networks on a small spatio-temporal scale. Specifically, we simultaneously assessed the effect of host plant availability, habitat complexity and seasonality on the structure of plant-herbivore networks in a coastal tropical ecosystem. Our results revealed that changes in the host plant community resulting from seasonality and habitat structure are reflected not only in the herbivore community, but also in the emergent properties (network parameters) of the plant-herbivore interaction network such as connectance, selectiveness and modularity. Habitat conditions and periods that are most stressful favored the presence of less selective and susceptible herbivore species, resulting in increased connectance within networks. In contrast, the high degree of selectivennes (i.e. interaction specialization) and modularity of the networks under less stressful conditions was promoted by the diversification in resource use by herbivores. By analyzing networks at a small spatio-temporal scale we identified the ecological factors structuring this network such as habitat complexity and seasonality. Our research offers new evidence on the role of abiotic and biotic factors in the variation of the properties of species interaction networks. PMID:25340790

PID-controller with predictor and auto-tuning algorithm: study of efficiency for thermal plants

NASA Astrophysics Data System (ADS)

Kuzishchin, V. F.; Merzlikina, E. I.; Hoang, Van Va

2017-09-01

The problem of efficiency estimation of an automatic control system (ACS) with a Smith predictor and PID-algorithm for thermal plants is considered. In order to use the predictor, it is proposed to include an auto-tuning module (ATC) into the controller; the module calculates parameters for a second-order plant module with a time delay. The study was conducted using programmable logical controllers (PLC), one of which performed control, ATC, and predictor functions. A simulation model was used as a control plant, and there were two variants of the model: one of them was built on the basis of a separate PLC, and the other was a physical model of a thermal plant in the form of an electrical heater. Analysis of the efficiency of the ACS with the predictor was carried out for several variants of the second order plant model with time delay, and the analysis was performed on the basis of the comparison of transient processes in the system when the set point was changed and when a disturbance influenced the control plant. The recommendations are given on correction of the PID-algorithm parameters when the predictor is used by means of using the correcting coefficient k for the PID parameters. It is shown that, when the set point is changed, the use of the predictor is effective taking into account the parameters correction with k = 2. When the disturbances influence the plant, the use of the predictor is doubtful, because the transient process is too long. The reason for this is that, in the neighborhood of the zero frequency, the amplitude-frequency characteristic (AFC) of the system with the predictor has an ascent in comparison with the AFC of the system without the predictor.

[Relation between species distribution of plant community and soil factors under grazing in alpine meadow].

PubMed

Niu, Yu Jie; Yang, Si Wei; Wang, Gui Zhen; Liu, Li; Du, Guo Zhen; Hua, Li Min

2017-12-01

The research selected the alpine meadow located in the northeastern margin of the Qinghai-Tibet Plateau to study the changes of vegetation community and soil properties under different grazing intensities, as well as the quantitative relation between the distribution patterns of plant species and the physical and chemical properties of soil. The results showed that the grazing caused the differentiation of the initial vegetation community with the dominant plants, Elymus nutans and Stipa grandis. In the plots with high and low grazing intensities, the dominant plants had changed to Kobresia humilis and Melissitus ruthenica, and E. nutans and Poa crymophila, respectively. With the increase of grazing intensity, the plant richness, importance value and biomass were significantly decreased. The sequence of plant species importance value in each plot against grazing intensity could be fitted by a logarithmic model. The number of required plant species was reduced while the importance value of the remaining plant species accounted for 50% of the importance value in the whole vegetation community. The available P, available K, soil compaction, soil water content, stable infiltration rate and large aggregate index were significantly changed with grazing intensity, however, the changes were different. The CCA ordination showed that the soil compaction was the key factor affecting the distribution pattern of the plant species under grazing. The variance decomposition indicated that the soil factors together explained 30.5% of the distribution of the plant species, in particular the soil physical properties alone explained 22.8% of the distribution of the plant species, which had the highest rate of contribution to the plant species distribution. The soil physical properties affected the distribution pattern of plant species on grazed alpine meadow.

Evaluating impact level of different factors in environmental impact assessment for incinerator plants using GM (1, N) model.

PubMed

Pai, T Y; Chiou, R J; Wen, H H

2008-01-01

In this study, the impact levels in environmental impact assessment (EIA) reports of 10 incinerator plants were quantified and discussed. The relationship between the quantified impact levels and the plant scale factors of BeiTou, LiZe, BaLi, LuTsao, RenWu, PingTung, SiJhou and HsinChu were constructed, and the impact levels of the GangShan (GS) and YongKong (YK) plants were predicted using grey model GM (1, N). Finally, the effects of plant scale factors on impact levels were evaluated using grey model GM (1, N) too. According to the predicted results of GM, the relative errors of topography/geology/soil, air quality, hydrology/water quality, solid waste, noise, terrestrial fauna/flora, aquatic fauna/flora and traffic in the GS plant were 17%, 14%, 15%, 17%, 75%, 16%, 13%, and 37%, respectively. The relative errors of the same environmental items in the YK plant were 1%, 18%, 10%, 40%, 37%, 3%, 25% and 33%, respectively. According to GM (1, N), design capacity (DC) and heat value (HV) were the plant scale factors that affected the impact levels significantly in each environmental item, and thus were the most significant plant scale factors. GM (1, N) was effective in predicting the environmental impact and analyzing the reasonableness of the impact. If there is an EIA for a new incinerator plant to be reviewed in the future, the official committee of the Taiwan EPA could review the reasonableness of impact levels in EIA reports quickly.

Pythium invasion of plant-based life support systems: biological control and sources

NASA Technical Reports Server (NTRS)

Jenkins, D. G.; Cook, K. L.; Garland, J. L.; Board, K. F.; Sager, J. C. (Principal Investigator)

2000-01-01

Invasion of plant-based life support systems by plant pathogens could cause plant disease and disruption of life support capability. Root rot caused by the fungus, Pythium, was observed during tests of prototype plant growth systems containing wheat at the Kennedy Space Center (KSC). We conducted experiments to determine if the presence of complex microbial communities in the plant root zone (rhizosphere) resisted invasion by the Pythium species isolated from the wheat root. Rhizosphere inocula of different complexity (as assayed by community-level physiological profile: CLPP) were developed using a dilution/extinction approach, followed by growth in hydroponic rhizosphere. Pythium growth on wheat roots and concomitant decreases in plant growth were inversely related to the complexity of the inocula during 20-day experiments in static hydroponic systems. Pythium was found on the seeds of several different wheat cultivars used in controlled environmental studies, but it is unclear if the seed-borne fungal strain(s) were identical to the pathogenic strain recovered from the KSC studies. Attempts to control pathogens and their effects in hydroponic life support systems should include early inoculation with complex microbial communities, which is consistent with ecological theory.

FOREVER YOUNG FLOWER Negatively Regulates Ethylene Response DNA-Binding Factors by Activating an Ethylene-Responsive Factor to Control Arabidopsis Floral Organ Senescence and Abscission.

PubMed

Chen, Wei-Han; Li, Pei-Fang; Chen, Ming-Kun; Lee, Yung-I; Yang, Chang-Hsien

2015-08-01

In this study of Arabidopsis (Arabidopsis thaliana), we investigated the relationship between FOREVER YOUNG FLOWER (FYF) and Ethylene Response DNA-binding Factors (EDFs) and functionally analyzed a key FYF target, an Ethylene-Responsive Factor (ERF), that controls flower senescence/abscission. Ectopic expression of EDF1/2/3/4 caused promotion of flower senescence/abscission and the activation of the senescence-associated genes. The presence of a repressor domain in EDFs and the enhancement of the promotion of senescence/abscission in EDF1/2/3/4+SRDX (converting EDFs to strong repressors by fusion with the ERF-associated amphiphilic repression motif repression domain SRDX) transgenic plants suggested that EDFs act as repressors. The significant reduction of β-glucuronidase (GUS) expression by 35S:FYF in EDF1/2/3/4:GUS plants indicates that EDF1/2/3/4 functions downstream of FYF in regulating flower senescence/abscission. In this study, we also characterized an ERF gene, FOREVER YOUNG FLOWER UP-REGULATING FACTOR1 (FUF1), which is up-regulated by FYF during flower development. Ectopic expression of FUF1 caused similar delayed flower senescence/abscission as seen in 35S:FYF plants. This phenotype was correlated with deficient abscission zone formation, ethylene insensitivity, and down-regulation of EDF1/2/3/4 and abscission-associated genes in 35S:FUF1 flowers. In contrast, significant promotion of flower senescence/abscission and up-regulation of EDF1/2/3/4 were observed in 35S:FUF1+SRDX transgenic dominant-negative plants, in which FUF1 is converted to a potent repressor by fusion to an SRDX-suppressing motif. Thus, FUF1 acts as an activator in suppressing EDF1/2/3/4 function and senescence/abscission of the flowers. Our results reveal that FYF regulates flower senescence/abscission by negatively regulating EDF1/2/3/4, which is the downstream gene in the ethylene response, by activating FUF1 in Arabidopsis. © 2015 American Society of Plant Biologists. All Rights

Diffusible signal factor-dependent quorum sensing in pathogenic bacteria and its exploitation for disease control.

PubMed

Dow, J M

2017-01-01

Cell-to-cell signals of the diffusible signal factor (DSF) family are cis-2-unsaturated fatty acids of differing chain length and branching pattern. DSF signalling has been described in diverse bacteria to include plant and human pathogens where it acts to regulate functions such as biofilm formation, antibiotic tolerance and the production of virulence factors. DSF family signals can also participate in interspecies signalling with other bacteria and interkingdom signalling such as with the yeast Candida albicans. Interference with DSF signalling may afford new opportunities for the control of bacterial disease. Such strategies will depend in part on detailed knowledge of the molecular mechanisms underlying the processes of signal synthesis, perception and turnover. Here, I review both recent progress in understanding DSF signalling at the molecular level and prospects for translating this knowledge into approaches for disease control. © 2016 The Society for Applied Microbiology.

Environmental optimal control strategies based on plant canopy photosynthesis responses and greenhouse climate model

NASA Astrophysics Data System (ADS)

Deng, Lujuan; Xie, Songhe; Cui, Jiantao; Liu, Tao

2006-11-01

It is the essential goal of intelligent greenhouse environment optimal control to enhance income of cropper and energy save. There were some characteristics such as uncertainty, imprecision, nonlinear, strong coupling, bigger inertia and different time scale in greenhouse environment control system. So greenhouse environment optimal control was not easy and especially model-based optimal control method was more difficult. So the optimal control problem of plant environment in intelligent greenhouse was researched. Hierarchical greenhouse environment control system was constructed. In the first level data measuring was carried out and executive machine was controlled. Optimal setting points of climate controlled variable in greenhouse was calculated and chosen in the second level. Market analysis and planning were completed in third level. The problem of the optimal setting point was discussed in this paper. Firstly the model of plant canopy photosynthesis responses and the model of greenhouse climate model were constructed. Afterwards according to experience of the planting expert, in daytime the optimal goals were decided according to the most maximal photosynthesis rate principle. In nighttime on plant better growth conditions the optimal goals were decided by energy saving principle. Whereafter environment optimal control setting points were computed by GA. Compared the optimal result and recording data in real system, the method is reasonable and can achieve energy saving and the maximal photosynthesis rate in intelligent greenhouse

Aquatic Plant Control Research Program. Large-Scale Operations Management Test (LSOMT) of Insects and Pathogens for Control of Waterhyacinth in Louisiana. Volume 1. Results for 1979-1981.

DTIC Science & Technology

1985-01-01

RD-Ai56 759 AQUATIC PLANT CONTROL RESEARCH PROGRAM LARGE-SCALE 1/2 OPERATIONS MRNAGEMENT..(U) ARMY ENGINEER WATERAYS EXPERIMENT STATION VICKSBURG MS...PO Box 631, Vicksburg, Aquatic Plant Control Mississippi 39180-0631 and University of Research Program Tennessee-Chattanooga, Chattanooga, 12...19. KEY WORDS (Continue on reverse side if necesary nd identify by block number) - Aquatic plant control Louisiana Biological control Plant

Hyper-activation of the TCP4 transcription factor in Arabidopsis thaliana accelerates multiple aspects of plant maturation.

PubMed

Sarvepalli, Kavitha; Nath, Utpal

2011-08-01

Plant organs are initiated as primordial outgrowths, and require controlled cell division and differentiation to achieve their final size and shape. Superimposed on this is another developmental program that orchestrates the switch from vegetative to reproductive to senescence stages in the life cycle. These require sequential function of heterochronic regulators. Little is known regarding the coordination between organ and organismal growth in plants. The TCP gene family encodes transcription factors that control diverse developmental traits, and a subgroup of class II TCP genes regulate leaf morphogenesis. Absence of these genes results in large, crinkly leaves due to excess division, mainly at margins. It has been suggested that these class II TCPs modulate the spatio-temporal control of differentiation in a growing leaf, rather than regulating cell proliferation per se. However, the link between class II TCP action and cell growth has not been established. As loss-of-function mutants of individual TCP genes in Arabidopsis are not very informative due to gene redundancy, we generated a transgenic line that expressed a hyper-activated form of TCP4 in its endogenous expression domain. This resulted in premature onset of maturation and decreased cell proliferation, leading to much smaller leaves, with cup-shaped lamina in extreme cases. Further, the transgenic line initiated leaves faster than wild-type and underwent precocious reproductive maturation due to a shortened adult vegetative phase. Early senescence and severe fertility defects were also observed. Thus, hyper-activation of TCP4 revealed its role in determining the timing of crucial developmental events, both at the organ and organism level. © 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.

A Study of Vocational Education as a Factor in Selecting a Community for a Manufacturing Plant Site.

ERIC Educational Resources Information Center

Dean, Ernest H.

The purpose of the study was to determine the factors considered by industrialists as they select communities in which to locate their new manufacturing plants, and to place the relative importance upon vocational-technical education as a factor in the plant site selection process. The three populations consisted of 113 companies of varying sizes…

Benchmark simulation Model no 2 in Matlab-simulink: towards plant-wide WWTP control strategy evaluation.

PubMed

Vreck, D; Gernaey, K V; Rosen, C; Jeppsson, U

2006-01-01

In this paper, implementation of the Benchmark Simulation Model No 2 (BSM2) within Matlab-Simulink is presented. The BSM2 is developed for plant-wide WWTP control strategy evaluation on a long-term basis. It consists of a pre-treatment process, an activated sludge process and sludge treatment processes. Extended evaluation criteria are proposed for plant-wide control strategy assessment. Default open-loop and closed-loop strategies are also proposed to be used as references with which to compare other control strategies. Simulations indicate that the BM2 is an appropriate tool for plant-wide control strategy evaluation.

Soft-Starting Power-Factor Motor Controller

NASA Technical Reports Server (NTRS)

Nola, F. J.

1983-01-01

Three-phase power-factor controller with soft start is based on earlier version that does not control starting transients. Additional components serve to turn off "run" command signal and substitute gradual startup command signal during preset startup interval. Improved controller reduces large current surge that usually accompanies starting. Controller applies power smoothly, without causing motor vibrations.

Control strategies of atmospheric mercury emissions from coal-fired power plants in China.

PubMed

Tian, Hezhong; Wang, Yan; Cheng, Ke; Qu, Yiping; Hao, Jiming; Xue, Zhigang; Chai, Fahe

2012-05-01

Atmospheric mercury (Hg) emission from coal is one of the primary sources of anthropogenic discharge and pollution. China is one of the few countries in the world whose coal consumption constitutes about 70% of total primary energy, and over half of coals are burned directly for electricity generation. Atmospheric emissions of Hg and its speciation from coal-fired power plants are of great concern owing to their negative impacts on regional human health and ecosystem risks, as well as long-distance transport. In this paper, recent trends of atmospheric Hg emissions and its species split from coal-fired power plants in China during the period of 2000-2007 are evaluated, by integrating each plant's coal consumption and emission factors, which are classified by different subcategories of boilers, particulate matter (PM) and sulfur dioxide (SO2) control devices. Our results show that the total Hg emissions from coal-fired power plants have begun to decrease from the peak value of 139.19 t in 2005 to 134.55 t in 2007, though coal consumption growing steadily from 1213.8 to 1532.4 Mt, which can be mainly attributed to the co-benefit Hg reduction by electrostatic precipitators/fabric filters (ESPs/FFs) and wet flue gas desulfurization (WFGD), especially the sharp growth in installation of WFGD both in the new and existing power plants since 2005. In the coming 12th five-year-plan, more and more plants will be mandated to install De-NO(x) (nitrogen oxides) systems (mainly selective catalytic reduction [SCR] and selective noncatalytic reduction [SNCR]) for minimizing NO(x) emission, thus the specific Hg emission rate per ton of coal will decline further owing to the much higher co-benefit removal efficiency by the combination of SCR + ESPs/FFs + WFGD systems. Consequently, SCR + ESPs/FFs + WFGD configuration will be the main path to abate Hg discharge from coal-fired power plants in China in the near future. However advanced specific Hg removal technologies are necessary

Controlled ecological life support systems: Development of a plant growth module

NASA Technical Reports Server (NTRS)

Averner, Mel M.; Macelroy, Robert D.; Smernoff, David T.

1987-01-01

An effort was made to begin defining the scientific and technical requirements for the design and construction of a ground-based plant growth facility. In particular, science design criteria for the Plant Growth Module (PGM) of the Controlled Ecological Life Support System (CELSS) were determined in the following areas: (1) irradiation parameters and associated equipment affecting plant growth; (2) air flow; (3) planting, culture, and harvest techniques; (4) carbon dioxide; (5) temperature and relative humidity; (6) oxygen; (7) construction materials and access; (8) volatile compounds; (9) bacteria, sterilization, and filtration; (10) nutrient application systems; (11) nutrient monitoring; and (12) nutrient pH and conductivity.

Mechanisms and effective control of physiological browning phenomena in plant cell cultures.

PubMed

Dong, Yan-Shan; Fu, Chun-Hua; Su, Peng; Xu, Xiang-Ping; Yuan, Jie; Wang, Sheng; Zhang, Meng; Zhao, Chun-Fang; Yu, Long-Jiang

2016-01-01

Browning phenomena are ubiquitous in plant cell cultures that severely hamper scientific research and widespread application of plant cell cultures. Up to now, this problem still has not been well controlled due to the unclear browning mechanisms in plant cell cultures. In this paper, the mechanisms were investigated using two typical materials with severe browning phenomena, Taxus chinensis and Glycyrrhiza inflata cells. Our results illustrated that the browning is attributed to a physiological enzymatic reaction, and phenolic biosynthesis regulated by sugar plays a decisive role in the browning. Furthermore, to confirm the specific compounds which participate in the enzymatic browning reaction, transcriptional profile and metabolites of T. chinensis cells, and UV scanning and high-performance liquid chromatography-mass spectrometry (HPLC-MS) profile of the browning compounds extracted from the brown-turned medium were analyzed, flavonoids derived from phenylpropanoid pathway were found to be the main compounds, and myricetin and quercetin were deduced to be the main substrates of the browning reaction. Inhibition of flavonoid biosynthesis can prevent the browning occurrence, and the browning is effectively controlled via blocking flavonoid biosynthesis by gibberellic acid (GA3 ) as an inhibitor, which further confirms that flavonoids mainly contribute to the browning. On the basis above, a model elucidating enzymatic browning mechanisms in plant cell cultures was put forward, and effective control approaches were presented. © 2015 Scandinavian Plant Physiology Society.

Differential activity of autochthonous bacteria in controlling drought stress in native Lavandula and Salvia plants species under drought conditions in natural arid soil.

PubMed

Armada, Elisabeth; Roldán, Antonio; Azcon, Rosario

2014-02-01

The effectiveness of autochthonous plant growth-promoting rhizobacteria was studied in Lavandula dentata and Salvia officinalis growing in a natural arid Mediterranean soil under drought conditions. These bacteria identified as Bacillus megaterium (Bm), Enterobacter sp. (E), Bacillus thuringiensis (Bt), and Bacillus sp. (Bsp). Each bacteria has different potential to meliorate water limitation and alleviating drought stress in these two plant species. B. thuringiensis promoted growth and drought avoidance in Lavandula by increasing K content, by depressing stomatal conductance, and it controlled shoot proline accumulation. This bacterial effect on increasing drought tolerance was related to the decrease of glutathione reductase (GR) and ascorbate peroxidase (APX) that resulted sensitive indexes of lower cellular oxidative damage involved in the adaptative drought response in B. thuringiensis-inoculated Lavandula plants. In contrast, in Salvia, having intrinsic lower shoot/root ratio, higher stomatal conductance and lower APX and GR activities than Lavandula, the bacterial effects on nutritional, physiological and antioxidant enzymatic systems were lower. The benefit of bacteria depended on intrinsic stress tolerance of plant involved. Lavadula demonstrated a greater benefit than Salvia to control drought stress when inoculated with B. thuringiensis. The bacterial drought tolerance assessed as survival, proline, and indolacetic acid production showed the potential of this bacteria to help plants to grow under drought conditions. B. thuringiensis may be used for Lavandula plant establishment in arid environments. Particular characteristic of the plant species as low shoot/root ratio and high stomatal conductance are important factors controlling the bacterial effectiveness improving nutritional, physiological, and metabolic plant activities.

Plant species modifies the functional response of Phytoseiulus persimilis (Acari: Phytoseiidae) to Tetranychus urticae (Acari: Tetranychidae): implications for biological control.

PubMed

Skirvin, D J; Fenlon, J S

2001-02-01

The functional response of the predatory mite Phytoseiulus persimilis Athias-Henriot to eggs of its prey, the spider mite Tetranychus urticae Koch was examined on three plant species. Experiments were done to determine whether differences in the functional response on the three plant species were due to the morphological features of the crop directly on the predator or through an effect of the plant species on the prey. The results show that crop morphology is the only factor influencing the predatory ability of P. persimilis on the three plant species. Fewer eggs were eaten on Ceanothus thyrsiflorus var. 'Autumnal Blue', the plant species with hairy leaves, and greater numbers of prey consumed on Choisya ternata, a species with smooth leaves. However, similarly few eggs were eaten on the smooth, but waxy leaved Euonymus japonicus as on Ceanothus thyrsiflorus, demonstrating that morphological characters of leaves other than the possession of hairs and trichomes may affect the rates of predation. The implications of these results for the tritrophic interactions between plant, predator and prey, and the development of suitable biological control strategies are discussed.

Q&A: How do gene regulatory networks control environmental responses in plants?

PubMed

Sun, Ying; Dinneny, José R

2018-04-11

A gene regulatory network (GRN) describes the hierarchical relationship between transcription factors, associated proteins, and their target genes. Studying GRNs allows us to understand how a plant's genotype and environment are integrated to regulate downstream physiological responses. Current efforts in plants have focused on defining the GRNs that regulate functions such as development and stress response and have been performed primarily in genetically tractable model plant species such as Arabidopsis thaliana. Future studies will likely focus on how GRNs function in non-model plants and change over evolutionary time to allow for adaptation to extreme environments. This broader understanding will inform efforts to engineer GRNs to create tailored crop traits.

Instrumentation and control upgrade plan for Browns Ferry nuclear plant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Belew, M.R.; Langley, D.T.; Torok, R.C.

1992-01-01

A comprehensive upgrade of the instrumentation and control (I C) systems at a power plant represents a formidable project for any utility. For a nuclear plant, the extra safety and reliability requirements along with regulatory constraints add further complications and cost. The need for the upgrade must, therefore, be very compelling, and the process must be well planned from the start. This paper describes the steps taken to initiate the I C upgrade process for Tennessee Valley Authority's (TVA's) Browns Ferry 2 nuclear plant. It explains the impetus for the upgrade, the expected benefits, and the process by which systemmore » upgrades will be selected and implemented.« less

The Arabidopsis bZIP11 transcription factor links low-energy signalling to auxin-mediated control of primary root growth.

PubMed

Weiste, Christoph; Pedrotti, Lorenzo; Selvanayagam, Jebasingh; Muralidhara, Prathibha; Fröschel, Christian; Novák, Ondřej; Ljung, Karin; Hanson, Johannes; Dröge-Laser, Wolfgang

2017-02-01

Plants have to tightly control their energy homeostasis to ensure survival and fitness under constantly changing environmental conditions. Thus, it is stringently required that energy-consuming stress-adaptation and growth-related processes are dynamically tuned according to the prevailing energy availability. The evolutionary conserved SUCROSE NON-FERMENTING1 RELATED KINASES1 (SnRK1) and the downstream group C/S1 basic leucine zipper (bZIP) transcription factors (TFs) are well-characterised central players in plants' low-energy management. Nevertheless, mechanistic insights into plant growth control under energy deprived conditions remains largely elusive. In this work, we disclose the novel function of the low-energy activated group S1 bZIP11-related TFs as regulators of auxin-mediated primary root growth. Whereas transgenic gain-of-function approaches of these bZIPs interfere with the activity of the root apical meristem and result in root growth repression, root growth of loss-of-function plants show a pronounced insensitivity to low-energy conditions. Based on ensuing molecular and biochemical analyses, we propose a mechanistic model, in which bZIP11-related TFs gain control over the root meristem by directly activating IAA3/SHY2 transcription. IAA3/SHY2 is a pivotal negative regulator of root growth, which has been demonstrated to efficiently repress transcription of major auxin transport facilitators of the PIN-FORMED (PIN) gene family, thereby restricting polar auxin transport to the root tip and in consequence auxin-driven primary root growth. Taken together, our results disclose the central low-energy activated SnRK1-C/S1-bZIP signalling module as gateway to integrate information on the plant's energy status into root meristem control, thereby balancing plant growth and cellular energy resources.

Software Configuration Management Plan for the B-Plant Canyon Ventilation Control System

DOE Office of Scientific and Technical Information (OSTI.GOV)

MCDANIEL, K.S.

1999-08-31

Project W-059 installed a new B Plant Canyon Ventilation System. Monitoring and control of the system is implemented by the Canyon Ventilation Control System (CVCS). This Software Configuration Management Plan provides instructions for change control of the CVCS.

Updating Human Factors Engineering Guidelines for Conducting Safety Reviews of Nuclear Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

O, J.M.; Higgins, J.; Stephen Fleger - NRC

The U.S. Nuclear Regulatory Commission (NRC) reviews the human factors engineering (HFE) programs of applicants for nuclear power plant construction permits, operating licenses, standard design certifications, and combined operating licenses. The purpose of these safety reviews is to help ensure that personnel performance and reliability are appropriately supported. Detailed design review procedures and guidance for the evaluations is provided in three key documents: the Standard Review Plan (NUREG-0800), the HFE Program Review Model (NUREG-0711), and the Human-System Interface Design Review Guidelines (NUREG-0700). These documents were last revised in 2007, 2004 and 2002, respectively. The NRC is committed to the periodicmore » update and improvement of the guidance to ensure that it remains a state-of-the-art design evaluation tool. To this end, the NRC is updating its guidance to stay current with recent research on human performance, advances in HFE methods and tools, and new technology being employed in plant and control room design. This paper describes the role of HFE guidelines in the safety review process and the content of the key HFE guidelines used. Then we will present the methodology used to develop HFE guidance and update these documents, and describe the current status of the update program.« less

Power Factor Controller

NASA Technical Reports Server (NTRS)

1997-01-01

Frank Nola invented the Power Factor Controller (PFC) at Marshall Space Flight Center more than a decade ago. Nola came up with a way to curb power wastage in AC induction motors. The PFC matches voltage with the motor's actual need by continuously sensing shifts between voltage and current. When it senses a light load it cuts the voltage to the minimum needed. Potential energy savings range from 8 to 65 percent.

Modelling the effect of environmental factors on resource allocation in mixed plants systems

NASA Astrophysics Data System (ADS)

Gayler, Sebastian; Priesack, Eckart

2010-05-01

In most cases, growth of plants is determined by competition against neighbours for the local resources light, water and nutrients and by defending against herbivores and pathogens. Consequently, it is important for a plant to grow fast without neglecting defence. However, plant internal substrates and energy required to support maintenance, growth and defence are limited and the total demand for these processes cannot be met in most cases. Therefore, allocation of carbohydrates to growth related primary metabolism or to defence related secondary metabolism can be seen as a trade-off between the demand of plants for being competitive against neighbours and for being more resistant against pathogens. A modelling approach is presented which can be used to simulate competition for light, water and nutrients between plant individuals in mixed canopies. The balance of resource allocation between growth processes and synthesis of secondary compounds is modelled by a concept originating from different plant defence hypothesis. The model is used to analyse the impact of environmental factors such as soil water and nitrogen availability, planting density and atmospheric concentration of CO2 on growth of plant individuals within mixed canopies and variations in concentration of carbon-based secondary metabolites in plant tissues.

Phytocalpain controls the proliferation and differentiation fates of cells in plant organ development.

PubMed

Ahn, Joon-Woo; Kim, Moonil; Lim, Jeong Hwa; Kim, Gyung-Tae; Pai, Hyun-Sook

2004-06-01

Calpain, a calcium-dependent cysteine protease, plays an essential role in basic cellular processes in animal cells, including cell proliferation, apoptosis, and differentiation. NbDEK encodes the calpain homolog of N. benthamiana. In this study, virus-induced gene silencing (VIGS) of NbDEK resulted in arrested organ development and hyperplasia in all the major plant organs examined. The epidermal layers of the leaves and stems were covered with hyperproliferating cell masses, and stomata and trichome development was severely inhibited. During flower development, a single dome-like structure was grown from the flower meristem to generate a large cylinder-shaped flower lacking any floral organs. At the cellular level, cell division was sustained in tissues that were otherwise already differentiated, and cell differentiation was severely hampered. NbDEK is ubiquitously expressed in all the plant tissues examined. In the abnormal organs of the NbDEK VIGS lines, protein levels of D-type cyclins (CycD)2, CycD3, and proliferating cell nuclear antigen (PCNA) were greatly elevated, and transcription of E2F (E2 promoter binding factor), E2F-regulated genes, retinoblastoma (Rb), and KNOTTED1 (KN1)-type homeobox genes was also stimulated. These results suggest that phytocalpain is a key regulator of cell proliferation and differentiation during plant organogenesis, and that it acts partly by controlling the CycD/Rb pathway.

Overexpression of the PAP1 Transcription Factor Reveals a Complex Regulation of Flavonoid and Phenylpropanoid Metabolism in Nicotiana tabacum Plants Attacked by Spodoptera litura

PubMed Central

Mitsunami, Tomoko; Nishihara, Masahiro; Galis, Ivan; Alamgir, Kabir Md; Hojo, Yuko; Fujita, Kohei; Sasaki, Nobuhiro; Nemoto, Keichiro; Sawasaki, Tatsuya; Arimura, Gen-ichiro

2014-01-01

Anthocyanin pigments and associated flavonoids have demonstrated antioxidant properties and benefits for human health. Consequently, current plant bioengineers have focused on how to modify flavonoid metabolism in plants. Most of that research, however, does not consider the role of natural biotic stresses (e.g., herbivore attack). To understand the influence of herbivore attack on the metabolic engineering of flavonoids, we examined tobacco plants overexpressing the Arabidopsis PAP1 gene (encoding an MYB transcription factor), which accumulated anthocyanin pigments and other flavonoids/phenylpropanoids. In comparison to wild-type and control plants, transgenic plants exhibited greater resistance to Spodoptera litura. Moreover, herbivory suppressed the PAP1-induced increase of transcripts of flavonoid/phenylpropanoid biosynthetic genes (e.g., F3H) and the subsequent accumulation of these genes' metabolites, despite the unaltered PAP1 mRNA levels after herbivory. The instances of down-regulation were independent of the signaling pathways mediated by defense-related jasmonates but were relevant to the levels of PAP1-induced and herbivory-suppressed transcription factors, An1a and An1b. Although initially F3H transcripts were suppressed by herbivory, after the S. litura feeding was interrupted, F3H transcripts increased. We hypothesize that in transgenic plants responding to herbivory, there is a complex mechanism regulating enriched flavonoid/phenylpropanoid compounds, via biotic stress signals. PMID:25268129

Full State Feedback Control for Virtual Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, Jay Tillay

This report presents an object-oriented implementation of full state feedback control for virtual power plants (VPP). The components of the VPP full state feedback control are (1) objectoriented high-fidelity modeling for all devices in the VPP; (2) Distribution System Distributed Quasi-Dynamic State Estimation (DS-DQSE) that enables full observability of the VPP by augmenting actual measurements with virtual, derived and pseudo measurements and performing the Quasi-Dynamic State Estimation (QSE) in a distributed manner, and (3) automated formulation of the Optimal Power Flow (OPF) in real time using the output of the DS-DQSE, and solving the distributed OPF to provide the optimalmore » control commands to the DERs of the VPP.« less

MicroRNA and Transcription Factor: Key Players in Plant Regulatory Network

PubMed Central

Samad, Abdul F. A.; Sajad, Muhammad; Nazaruddin, Nazaruddin; Fauzi, Izzat A.; Murad, Abdul M. A.; Zainal, Zamri; Ismail, Ismanizan

2017-01-01

Recent achievements in plant microRNA (miRNA), a large class of small and non-coding RNAs, are very exciting. A wide array of techniques involving forward genetic, molecular cloning, bioinformatic analysis, and the latest technology, deep sequencing have greatly advanced miRNA discovery. A tiny miRNA sequence has the ability to target single/multiple mRNA targets. Most of the miRNA targets are transcription factors (TFs) which have paramount importance in regulating the plant growth and development. Various families of TFs, which have regulated a range of regulatory networks, may assist plants to grow under normal and stress environmental conditions. This present review focuses on the regulatory relationships between miRNAs and different families of TFs like; NF-Y, MYB, AP2, TCP, WRKY, NAC, GRF, and SPL. For instance NF-Y play important role during drought tolerance and flower development, MYB are involved in signal transduction and biosynthesis of secondary metabolites, AP2 regulate the floral development and nodule formation, TCP direct leaf development and growth hormones signaling. WRKY have known roles in multiple stress tolerances, NAC regulate lateral root formation, GRF are involved in root growth, flower, and seed development, and SPL regulate plant transition from juvenile to adult. We also studied the relation between miRNAs and TFs by consolidating the research findings from different plant species which will help plant scientists in understanding the mechanism of action and interaction between these regulators in the plant growth and development under normal and stress environmental conditions. PMID:28446918

MicroRNA and Transcription Factor: Key Players in Plant Regulatory Network.

PubMed

Samad, Abdul F A; Sajad, Muhammad; Nazaruddin, Nazaruddin; Fauzi, Izzat A; Murad, Abdul M A; Zainal, Zamri; Ismail, Ismanizan

2017-01-01

Recent achievements in plant microRNA (miRNA), a large class of small and non-coding RNAs, are very exciting. A wide array of techniques involving forward genetic, molecular cloning, bioinformatic analysis, and the latest technology, deep sequencing have greatly advanced miRNA discovery. A tiny miRNA sequence has the ability to target single/multiple mRNA targets. Most of the miRNA targets are transcription factors (TFs) which have paramount importance in regulating the plant growth and development. Various families of TFs, which have regulated a range of regulatory networks, may assist plants to grow under normal and stress environmental conditions. This present review focuses on the regulatory relationships between miRNAs and different families of TFs like; NF-Y, MYB, AP2, TCP, WRKY, NAC, GRF, and SPL. For instance NF-Y play important role during drought tolerance and flower development, MYB are involved in signal transduction and biosynthesis of secondary metabolites, AP2 regulate the floral development and nodule formation, TCP direct leaf development and growth hormones signaling. WRKY have known roles in multiple stress tolerances, NAC regulate lateral root formation, GRF are involved in root growth, flower, and seed development, and SPL regulate plant transition from juvenile to adult. We also studied the relation between miRNAs and TFs by consolidating the research findings from different plant species which will help plant scientists in understanding the mechanism of action and interaction between these regulators in the plant growth and development under normal and stress environmental conditions.

Widespread mechanosensing controls the structure behind the architecture in plants.

PubMed

Hamant, Olivier

2013-10-01

Mechanical forces play an instructing role for many aspects of animal cell biology, such as division, polarity and fate. Although the associated mechanoperception pathways still remain largely elusive in plants, physical cues have long been thought to guide development in parallel to biochemical factors. With the development of new imaging techniques, micromechanics tools and modeling approaches, the role of mechanical signals in plant development is now re-examined and fully integrated with modern cell biology. Using recent examples from the literature, I propose to use a multiscale perspective, from the whole plant down to the cell wall, to fully appreciate the diversity of developmental processes that depend on mechanical signals. Incidentally, this also illustrates how conceptually rich this field is. Copyright © 2013 Elsevier Ltd. All rights reserved.

Microbiome studies in the biological control of plant pathogens

USDA-ARS?s Scientific Manuscript database

Biological control of plant pathogens, although it has been a successful alternative that has allowed to select microorganisms for the generation of bioproducts and to understand multiple biological mechanisms, cannot be considered as a strategy defined only from the selection of a range of cultiva...

Dynamic Modeling and Plantwide Control of a Hybrid Power and Chemical Plant: An Integrated Gasification Combined Cycle Coupled with a Methanol Plant

NASA Astrophysics Data System (ADS)

Robinson, Patrick J.

Gasification has been used in industry on a relatively limited scale for many years, but it is emerging as the premier unit operation in the energy and chemical industries. The switch from expensive and insecure petroleum to solid hydrocarbon sources (coal and biomass) is occurring due to the vast amount of domestic solid resources, national security and global warming issues. Gasification (or partial oxidation) is a vital component of "clean coal" technology. Sulfur and nitrogen emissions can be reduced, overall energy efficiency is increased and carbon dioxide recovery and sequestration are facilitated. Gasification units in an electric power generation plant produce a fuel gas for driving combustion turbines. Gasification units in a chemical plant generate synthesis gas, which can be used to produce a wide spectrum of chemical products. Future plants are predicted to be hybrid power/chemical plants with gasification as the key unit operation. The coupling of an Integrated Gasification Combined Cycle (IGCC) with a methanol plant can handle swings in power demand by diverting hydrogen gas from a combustion turbine and synthesis gas from the gasifier to a methanol plant for the production of an easily-stored, hydrogen-consuming liquid product. An additional control degree of freedom is provided with this hybrid plant, fundamentally improving the controllability of the process. The idea is to base-load the gasifier and use the more responsive gas-phase units to handle disturbances. During the summer days, power demand can fluctuate up to 50% over a 12-hour period. The winter provides a different problem where spikes of power demand can go up 15% within the hour. The following dissertation develops a hybrid IGCC / methanol plant model, validates the steady-state results with a National Energy Technical Laboratory study, and tests a proposed control structure to handle these significant disturbances. All modeling was performed in the widely used chemical process

Disentangling the influence of environmental and anthropogenic factors on the distribution of endemic vascular plants in Sardinia.

PubMed

Fois, Mauro; Fenu, Giuseppe; Cañadas, Eva Maria; Bacchetta, Gianluigi

2017-01-01

Due to the impelling urgency of plant conservation and the increasing availability of high resolution spatially interpolated (e.g. climate variables) and categorical data (e.g. land cover and vegetation type), many recent studies have examined relationships among plant species distributions and a diversified set of explanatory factors; nevertheless, global and regional patterns of endemic plant richness remain in many cases unexplained. One such pattern is the 294 endemic vascular plant taxa recorded on a 1 km resolution grid on the environmentally heterogeneous island of Sardinia. Sixteen predictors, including topographic, geological, climatic and anthropogenic factors, were used to model local (number of taxa inside each 1 km grid cell) Endemic Vascular Plant Richness (EVPR). Generalized Linear Models were used to evaluate how each factor affected the distribution of local EVPR. Significant relationships with local EVPR and topographic, geological, climatic and anthropogenic factors were found. In particular, elevation explained the larger fraction of variation in endemic richness but other environmental factors (e.g. precipitation seasonality and slope) and human-related factors (e.g. the Human Influence Index (HII) and the proportion of anthropogenic land uses) were, respectively, positively and negatively correlated with local EVPR. Regional EVPR (number of endemic taxa inside each 100 m elevation interval) was also measured to compare local and regional EVPR patterns along the elevation gradient. In contrast to local, regional EVPR tended to decrease with altitude partly due to the decreasing area covered along altitude. The contrasting results between local and regional patterns suggest that local richness increases as a result of increased interspecific aggregation along altitude, whereas regional richness may depend on the interaction between area and altitude. This suggests that the shape and magnitude of the species-area relationship might vary with

Integration of Plant Defense Traits with Biological Control of Arthropod Pests: Challenges and Opportunities

PubMed Central

Peterson, Julie A.; Ode, Paul J.; Oliveira-Hofman, Camila; Harwood, James D.

2016-01-01

Crop plants exhibit a wide diversity of defensive traits and strategies to protect themselves from damage by herbivorous pests and disease. These defensive traits may be naturally occurring or artificially selected through crop breeding, including introduction via genetic engineering. While these traits can have obvious and direct impacts on herbivorous pests, many have profound effects on higher trophic levels, including the natural enemies of herbivores. Multi-trophic effects of host plant resistance have the potential to influence, both positively and negatively, biological control. Plant defense traits can influence both the numerical and functional responses of natural enemies; these interactions can be semiochemically, plant toxin-, plant nutrient-, and/or physically mediated. Case studies involving predators, parasitoids, and pathogens of crop pests will be presented and discussed. These diverse groups of natural enemies may respond differently to crop plant traits based on their own unique biology and the ecological niches they fill. Genetically modified crop plants that have been engineered to express transgenic products affecting herbivorous pests are an additional consideration. For the most part, transgenic plant incorporated protectant (PIP) traits are compatible with biological control due to their selective toxicity to targeted pests and relatively low non-target impacts, although transgenic crops may have indirect effects on higher trophic levels and arthropod communities mediated by lower host or prey number and/or quality. Host plant resistance and biological control are two of the key pillars of integrated pest management; their potential interactions, whether they are synergistic, complementary, or disruptive, are key in understanding and achieving sustainable and effective pest management. PMID:27965695

Integration of Plant Defense Traits with Biological Control of Arthropod Pests: Challenges and Opportunities.

PubMed

Peterson, Julie A; Ode, Paul J; Oliveira-Hofman, Camila; Harwood, James D

2016-01-01

Crop plants exhibit a wide diversity of defensive traits and strategies to protect themselves from damage by herbivorous pests and disease. These defensive traits may be naturally occurring or artificially selected through crop breeding, including introduction via genetic engineering. While these traits can have obvious and direct impacts on herbivorous pests, many have profound effects on higher trophic levels, including the natural enemies of herbivores. Multi-trophic effects of host plant resistance have the potential to influence, both positively and negatively, biological control. Plant defense traits can influence both the numerical and functional responses of natural enemies; these interactions can be semiochemically, plant toxin-, plant nutrient-, and/or physically mediated. Case studies involving predators, parasitoids, and pathogens of crop pests will be presented and discussed. These diverse groups of natural enemies may respond differently to crop plant traits based on their own unique biology and the ecological niches they fill. Genetically modified crop plants that have been engineered to express transgenic products affecting herbivorous pests are an additional consideration. For the most part, transgenic plant incorporated protectant (PIP) traits are compatible with biological control due to their selective toxicity to targeted pests and relatively low non-target impacts, although transgenic crops may have indirect effects on higher trophic levels and arthropod communities mediated by lower host or prey number and/or quality. Host plant resistance and biological control are two of the key pillars of integrated pest management; their potential interactions, whether they are synergistic, complementary, or disruptive, are key in understanding and achieving sustainable and effective pest management.

The WRKY Transcription Factor WRKY71/EXB1 Controls Shoot Branching by Transcriptionally Regulating RAX Genes in Arabidopsis.

PubMed

Guo, Dongshu; Zhang, Jinzhe; Wang, Xinlei; Han, Xiang; Wei, Baoye; Wang, Jianqiao; Li, Boxun; Yu, Hao; Huang, Qingpei; Gu, Hongya; Qu, Li-Jia; Qin, Genji

2015-11-01

Plant shoot branching is pivotal for developmental plasticity and crop yield. The formation of branch meristems is regulated by several key transcription factors including REGULATOR OF AXILLARY MERISTEMS1 (RAX1), RAX2, and RAX3. However, the regulatory network of shoot branching is still largely unknown. Here, we report the identification of EXCESSIVE BRANCHES1 (EXB1), which affects axillary meristem (AM) initiation and bud activity. Overexpression of EXB1 in the gain-of-function mutant exb1-D leads to severe bushy and dwarf phenotypes, which result from excessive AM initiation and elevated bud activities. EXB1 encodes the WRKY transcription factor WRKY71, which has demonstrated transactivation activities. Disruption of WRKY71/EXB1 by chimeric repressor silencing technology leads to fewer branches, indicating that EXB1 plays important roles in the control of shoot branching. We demonstrate that EXB1 controls AM initiation by positively regulating the transcription of RAX1, RAX2, and RAX3. Disruption of the RAX genes partially rescues the branching phenotype caused by EXB1 overexpression. We further show that EXB1 also regulates auxin homeostasis in control of shoot branching. Our data demonstrate that EXB1 plays pivotal roles in shoot branching by regulating both transcription of RAX genes and auxin pathways. © 2015 American Society of Plant Biologists. All rights reserved.

Mechanosensitive control of plant growth: bearing the load, sensing, transducing, and responding

PubMed Central

Moulia, Bruno; Coutand, Catherine; Julien, Jean-Louis

2015-01-01

As land plants grow and develop, they encounter complex mechanical challenges, especially from winds and turgor pressure. Mechanosensitive control over growth and morphogenesis is an adaptive trait, reducing the risks of breakage or explosion. This control has been mostly studied through experiments with artificial mechanical loads, often focusing on cellular or molecular mechanotransduction pathway. However, some important aspects of mechanosensing are often neglected. (i) What are the mechanical characteristics of different loads and how are loads distributed within different organs? (ii) What is the relevant mechanical stimulus in the cell? Is it stress, strain, or energy? (iii) How do mechanosensing cells signal to meristematic cells? Without answers to these questions we cannot make progress analyzing the mechanobiological effects of plant size, plant shape, tissue distribution and stiffness, or the magnitude of stimuli. This situation is rapidly changing however, as systems mechanobiology is being developed, using specific biomechanical and/or mechanobiological models. These models are instrumental in comparing loads and responses between experiments and make it possible to quantitatively test biological hypotheses describing the mechanotransduction networks. This review is designed for a general plant science audience and aims to help biologists master the models they need for mechanobiological studies. Analysis and modeling is broken down into four steps looking at how the structure bears the load, how the distributed load is sensed, how the mechanical signal is transduced, and then how the plant responds through growth. Throughout, two examples of adaptive responses are used to illustrate this approach: the thigmorphogenetic syndrome of plant shoots bending and the mechanosensitive control of shoot apical meristem (SAM) morphogenesis. Overall this should provide a generic understanding of systems mechanobiology at work. PMID:25755656

MYB and bHLH transcription factor transgenes increase anthocyanin pigmentation in petunia and lisianthus plants, and the petunia phenotypes are strongly enhanced under field conditions

PubMed Central

Schwinn, Kathy E.; Boase, Murray R.; Bradley, J. Marie; Lewis, David H.; Deroles, Simon C.; Martin, Cathie R.; Davies, Kevin M.

2014-01-01

Petunia line Mitchell [MP, Petunia axillaris × (P. axillaris × P. hybrida)] and Eustoma grandiflorum (lisianthus) plants were produced containing a transgene for over-expression of the R2R3-MYB transcription factor [TF; ROSEA1 (ROS1)] that up-regulates flavonoid biosynthesis in Antirrhinum majus. The petunia lines were also crossed with previously produced MP lines containing a Zea mays flavonoid-related basic helix-loop-helix TF transgene (LEAF COLOR, LC), which induces strong vegetative pigmentation when these 35S:LC plants are exposed to high-light levels. 35S:ROS1 lisianthus transgenics had limited changes in anthocyanin pigmentation, specifically, precocious pigmentation of flower petals and increased pigmentation of sepals. RNA transcript levels for two anthocyanin biosynthetic genes, chalcone synthase and anthocyanidin synthase, were increased in the 35S:ROS1 lisianthus petals compared to those of control lines. With MP, the 35S:ROS1 calli showed novel red pigmentation in culture, but this was generally not seen in tissue culture plantlets regenerated from the calli or young plants transferred to soil in the greenhouse. Anthocyanin pigmentation was enhanced in the stems of mature 35S:ROS1 MP plants, but the MP white-flower phenotype was not complemented. Progeny from a 35S:ROS1 × 35S:LC cross had novel pigmentation phenotypes that were not present in either parental line or MP. In particular, there was increased pigment in the petal throat region, and the anthers changed from yellow to purple pigmentation. An outdoor field trial was conducted with the 35S:ROS1, 35S:LC, 35S:ROS1 × 35S:LC and control MP lines. Field conditions rapidly induced intense foliage pigmentation in 35S:LC plants, a phenotype not observed in control MP or equivalent 35S:LC plants maintained in a greenhouse. No difference in plant stature, seed germination, or plant survival was observed between transgenic and control plants. PMID:25414715

MYB and bHLH transcription factor transgenes increase anthocyanin pigmentation in petunia and lisianthus plants, and the petunia phenotypes are strongly enhanced under field conditions.

PubMed

Schwinn, Kathy E; Boase, Murray R; Bradley, J Marie; Lewis, David H; Deroles, Simon C; Martin, Cathie R; Davies, Kevin M

2014-01-01

Petunia line Mitchell [MP, Petunia axillaris × (P. axillaris × P. hybrida)] and Eustoma grandiflorum (lisianthus) plants were produced containing a transgene for over-expression of the R2R3-MYB transcription factor [TF; ROSEA1 (ROS1)] that up-regulates flavonoid biosynthesis in Antirrhinum majus. The petunia lines were also crossed with previously produced MP lines containing a Zea mays flavonoid-related basic helix-loop-helix TF transgene (LEAF COLOR, LC), which induces strong vegetative pigmentation when these 35S:LC plants are exposed to high-light levels. 35S:ROS1 lisianthus transgenics had limited changes in anthocyanin pigmentation, specifically, precocious pigmentation of flower petals and increased pigmentation of sepals. RNA transcript levels for two anthocyanin biosynthetic genes, chalcone synthase and anthocyanidin synthase, were increased in the 35S:ROS1 lisianthus petals compared to those of control lines. With MP, the 35S:ROS1 calli showed novel red pigmentation in culture, but this was generally not seen in tissue culture plantlets regenerated from the calli or young plants transferred to soil in the greenhouse. Anthocyanin pigmentation was enhanced in the stems of mature 35S:ROS1 MP plants, but the MP white-flower phenotype was not complemented. Progeny from a 35S:ROS1 × 35S:LC cross had novel pigmentation phenotypes that were not present in either parental line or MP. In particular, there was increased pigment in the petal throat region, and the anthers changed from yellow to purple pigmentation. An outdoor field trial was conducted with the 35S:ROS1, 35S:LC, 35S:ROS1 × 35S:LC and control MP lines. Field conditions rapidly induced intense foliage pigmentation in 35S:LC plants, a phenotype not observed in control MP or equivalent 35S:LC plants maintained in a greenhouse. No difference in plant stature, seed germination, or plant survival was observed between transgenic and control plants.

Factors influencing warfarin control in Australia and Singapore.

PubMed

Bernaitis, Nijole; Ching, Chi Keong; Teo, Siew Chong; Chen, Liping; Badrick, Tony; Davey, Andrew K; Crilly, Julia; Anoopkumar-Dukie, Shailendra

2017-09-01

Warfarin is widely used for patients with non-valvular atrial fibrillation (NVAF). Variations in warfarin control, as measured by time in therapeutic range (TTR), have been reported across different regions and ethnicities, particularly between Western and Asian countries. However, there is limited data on comparative factors influencing warfarin control in Caucasian and Asian patients. Therefore, the aim of this study was to determine warfarin control and potential factors influencing this in patients with NVAF in Australia and Singapore. Retrospective data was collected for patients receiving warfarin for January to June 2014 in Australia and Singapore. TTR was calculated for individuals with mean patient TTR used for analysis. Possible influential factors on TTR were analysed including age, gender, concurrent co-morbidities, and concurrent medication. The mean TTR was significantly higher in Australia (82%) than Singapore (58%). At both sites, chronic kidney disease significantly lowered this TTR. Further factors influencing control were anaemia and age<60years in Australia, and vascular disease, CHA 2 DS 2 -VASc score of 6, and concurrent platelet inhibitor therapy in Singapore. Warfarin control was significantly higher in Australia compared to Singapore, however chronic kidney disease reduced control at both sites. The different levels of control in these two countries, together with patient factors further reducing control may impact on anticoagulant choice in these countries with better outcomes from warfarin in Australia compared to Singapore. Copyright © 2017 Elsevier Ltd. All rights reserved.

Gaseous emissions from plants in controlled environments

NASA Technical Reports Server (NTRS)

Dubay, Denis T.

1988-01-01

Plant growth in a controlled ecological life support system may entail the build-up over extended time periods of phytotoxic concentrations of volatile organic compounds produced by the plants themselves. Ethylene is a prominent gaseous emission of plants, and is the focus of this report. The objective was to determine the rate of ethylene release by spring wheat, white potato, and lettuce during early, middle, and late growth stages, and during both the light and dark segments of the diurnal cycle. Plants grown hydroponically using the nutrient film technique were covered with plexiglass containers for 4 to 6 h. At intervals after enclosure, gas samples were withdrawn with a syringe and analyzed for ethylene with a gas chromatograph. Lettuce produced 10 to 100 times more ethylene than wheat or potato, with production rates ranging from 141 to 158 ng g-dry/wt/h. Wheat produced from 1.7 to 14.3 ng g-dry/wt/h, with senescent wheat producing the least amount and flowering wheat the most. Potatoes produced the least amount of ethylene, with values never exceeding 5 ng g-dry/wt/h. Lettuce and potatoes each produced ethylene at similar rates whether in dark period or light period. Ethylene sequestering of 33 to 43 percent by the plexiglass enclosures indicated that these production estimates may be low by one-third to one-half. These results suggest that concern for ethylene build-up in a contained atmosphere should be greatest when growing lettuce, and less when growing wheat or potato.

Response of pest control by generalist predators to local-scale plant diversity: a meta-analysis.

PubMed

Dassou, Anicet Gbèblonoudo; Tixier, Philippe

2016-02-01

Disentangling the effects of plant diversity on the control of herbivores is important for understanding agricultural sustainability. Recent studies have investigated the relationships between plant diversity and arthropod communities at the landscape scale, but few have done so at the local scale. We conducted a meta-analysis of 32 papers containing 175 independent measures of the relationship between plant diversity and arthropod communities. We found that generalist predators had a strong positive response to plant diversity, that is, their abundance increased as plant diversity increased. Herbivores, in contrast, had an overall weak and negative response to plant diversity. However, specialist and generalist herbivores differed in their response to plant diversity, that is, the response was negative for specialists and not significant for generalists. While the effects of scale remain unclear, the response to plant diversity tended to increase for specialist herbivores, but decrease for generalist herbivores as the scale increased. There was no clear effect of scale on the response of generalist predators to plant diversity. Our results suggest that the response of herbivores to plant diversity at the local scale is a balance between habitat and trophic effects that vary according to arthropod specialization and habitat type. Synthesis and applications. Positive effects of plant diversity on generalist predators confirm that, at the local scale, plant diversification of agroecosystems is a credible and promising option for increasing pest regulation. Results from our meta-analysis suggest that natural control in plant-diversified systems is more likely to occur for specialist than for generalist herbivores. In terms of pest management, our results indicate that small-scale plant diversification (via the planting of cover crops or intercrops and reduced weed management) is likely to increase the control of specialist herbivores by generalist predators.

Museum specimens reveal loss of pollen host plants as key factor driving wild bee decline in The Netherlands.

PubMed

Scheper, Jeroen; Reemer, Menno; van Kats, Ruud; Ozinga, Wim A; van der Linden, Giel T J; Schaminée, Joop H J; Siepel, Henk; Kleijn, David

2014-12-09

Evidence for declining populations of both wild and managed bees has raised concern about a potential global pollination crisis. Strategies to mitigate bee loss generally aim to enhance floral resources. However, we do not really know whether loss of preferred floral resources is the key driver of bee decline because accurate assessment of host plant preferences is difficult, particularly for species that have become rare. Here we examine whether population trends of wild bees in The Netherlands can be explained by trends in host plants, and how this relates to other factors such as climate change. We determined host plant preference of bee species using pollen loads on specimens in entomological collections that were collected before the onset of their decline, and used atlas data to quantify population trends of bee species and their host plants. We show that decline of preferred host plant species was one of two main factors associated with bee decline. Bee body size, the other main factor, was negatively related to population trend, which, because larger bee species have larger pollen requirements than smaller species, may also point toward food limitation as a key factor driving wild bee loss. Diet breadth and other potential factors such as length of flight period or climate change sensitivity were not important in explaining twentieth century bee population trends. These results highlight the species-specific nature of wild bee decline and indicate that mitigation strategies will only be effective if they target the specific host plants of declining species.

Museum specimens reveal loss of pollen host plants as key factor driving wild bee decline in The Netherlands

PubMed Central

Scheper, Jeroen; Reemer, Menno; van Kats, Ruud; Ozinga, Wim A.; van der Linden, Giel T. J.; Schaminée, Joop H. J.; Siepel, Henk; Kleijn, David

2014-01-01

Evidence for declining populations of both wild and managed bees has raised concern about a potential global pollination crisis. Strategies to mitigate bee loss generally aim to enhance floral resources. However, we do not really know whether loss of preferred floral resources is the key driver of bee decline because accurate assessment of host plant preferences is difficult, particularly for species that have become rare. Here we examine whether population trends of wild bees in The Netherlands can be explained by trends in host plants, and how this relates to other factors such as climate change. We determined host plant preference of bee species using pollen loads on specimens in entomological collections that were collected before the onset of their decline, and used atlas data to quantify population trends of bee species and their host plants. We show that decline of preferred host plant species was one of two main factors associated with bee decline. Bee body size, the other main factor, was negatively related to population trend, which, because larger bee species have larger pollen requirements than smaller species, may also point toward food limitation as a key factor driving wild bee loss. Diet breadth and other potential factors such as length of flight period or climate change sensitivity were not important in explaining twentieth century bee population trends. These results highlight the species-specific nature of wild bee decline and indicate that mitigation strategies will only be effective if they target the specific host plants of declining species. PMID:25422416

Chemical Weed Control Increases Survival and Growth in Hardwood Plantings

Treesearch

Gayne G. Erdmann

1967-01-01

In a plantation of four hardwood species on a silt loam soil planted to 1-0 stock, 4 pounds of active atrazine or simazine controlled weeds effectively without injuring the trees. Chemical weed control was better on plowed and disked ground than on unprepared ground. Yellow-poplar and white ash grew faster on prepared ground. Black walnut and red oak did not respond...

Unraveling the Plant-Soil Interactome

NASA Astrophysics Data System (ADS)

Lipton, M. S.; Hixson, K.; Ahkami, A. H.; HaHandkumbura, P. P.; Hess, N. J.; Fang, Y.; Fortin, D.; Stanfill, B.; Yabusaki, S.; Engbrecht, K. M.; Baker, E.; Renslow, R.; Jansson, C.

2017-12-01

Plant photosynthesis is the primary conduit of carbon fixation from the atmosphere to the terrestrial ecosystem. While more is known about plant physiology and biochemistry, the interplay between genetic and environmental factors that govern partitioning of carbon to above- and below ground plant biomass, to microbes, to the soil, and respired to the atmosphere is not well understood holistically. To address this knowledge gap there is a need to define, study, comprehend, and model the plant ecosystem as an integrated system of integrated biotic and abiotic processes and feedbacks. Local rhizosphere conditions are an important control on plant performance but are in turn affected by plant uptake and rhizodeposition processes. C3 and C4 plants have different CO2 fixation strategies and likely have differential metabolic profiles resulting in different carbon sources exuding to the rhizosphere. In this presentation, we report on an integrated capability to better understand plant-soil interactions, including modeling tools that address the spatiotemporal hydrobiogeochemistry in the rhizosphere. Comparing Brachypodium distachyon, (Brachypodium) as our C3 representative and Setaria viridis (Setaria) as our C4 representative, we designed, highly controlled single-plant experimental ecosystems based these model grasses to enable quantitative prediction of ecosystem traits and responses as a function of plant genotype and environmental variables. A metabolomics survey of 30 Brachypodium genotypes grown under control and drought conditions revealed specific metabolites that correlated with biomass production and drought tolerance. A comparison of Brachypodium and Setaria grown with control and a future predicted elevated CO2 level revealed changes in biomass accumulation and metabolite profiles between the C3 and C4 species in both leaves and roots. Finally, we are building an mechanistic modeling capability that will contribute to a better basis for modeling plant water

Advanced Grid-Friendly Controls Demonstration Project for Utility-Scale PV Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gevorgian, Vahan; O'Neill, Barbara

A typical photovoltaic (PV) power plant consists of multiple power electronic inverters and can contribute to grid stability and reliability through sophisticated 'grid-friendly' controls. The availability and dissemination of actual test data showing the viability of advanced utility-scale PV controls among all industry stakeholders can leverage PV's value from being simply an energy resource to providing additional ancillary services that range from variability smoothing and frequency regulation to power quality. Strategically partnering with a selected utility and/or PV power plant operator is a key condition for a successful demonstration project. The U.S. Department of Energy's (DOE's) Solar Energy Technologies Officemore » selected the National Renewable Energy Laboratory (NREL) to be a principal investigator in a two-year project with goals to (1) identify a potential partner(s), (2) develop a detailed scope of work and test plan for a field project to demonstrate the gird-friendly capabilities of utility-scale PV power plants, (3) facilitate conducting actual demonstration tests, and (4) disseminate test results among industry stakeholders via a joint NREL/DOE publication and participation in relevant technical conferences. The project implementation took place in FY 2014 and FY 2015. In FY14, NREL established collaborations with AES and First Solar Electric, LLC, to conduct demonstration testing on their utility-scale PV power plants in Puerto Rico and Texas, respectively, and developed test plans for each partner. Both Puerto Rico Electric Power Authority and the Electric Reliability Council of Texas expressed interest in this project because of the importance of such advanced controls for the reliable operation of their power systems under high penetration levels of variable renewable generation. During FY15, testing was completed on both plants, and a large amount of test data was produced and analyzed that demonstrates the ability of PV power plants

Simulation model for plant growth in controlled environment systems

NASA Technical Reports Server (NTRS)

Raper, C. D., Jr.; Wann, M.

1986-01-01

The role of the mathematical model is to relate the individual processes to environmental conditions and the behavior of the whole plant. Using the controlled-environment facilities of the phytotron at North Carolina State University for experimentation at the whole-plant level and methods for handling complex models, researchers developed a plant growth model to describe the relationships between hierarchial levels of the crop production system. The fundamental processes that are considered are: (1) interception of photosynthetically active radiation by leaves, (2) absorption of photosynthetically active radiation, (3) photosynthetic transformation of absorbed radiation into chemical energy of carbon bonding in solube carbohydrates in the leaves, (4) translocation between carbohydrate pools in leaves, stems, and roots, (5) flow of energy from carbohydrate pools for respiration, (6) flow from carbohydrate pools for growth, and (7) aging of tissues. These processes are described at the level of organ structure and of elementary function processes. The driving variables of incident photosynthetically active radiation and ambient temperature as inputs pertain to characterization at the whole-plant level. The output of the model is accumulated dry matter partitioned among leaves, stems, and roots; thus, the elementary processes clearly operate under the constraints of the plant structure which is itself the output of the model.

A Plant 5S Ribosomal RNA Mimic Regulates Alternative Splicing of Transcription Factor IIIA Pre-mRNAs

PubMed Central

Hammond, Ming C.; Wachter, Andreas; Breaker, Ronald R.

2009-01-01

Transcription factor IIIA (TFIIIA) is required for eukaryotic synthesis of 5S ribosomal RNA by RNA polymerase III. Here we report the discovery of a structured RNA element with striking resemblance to 5S rRNA that is conserved within TFIIIA precursor mRNAs (pre-mRNAs) from diverse plant lineages. TFIIIA protein expression is controlled by alternative splicing of the exon containing the plant 5S rRNA mimic (P5SM). P5SM triggers exon skipping upon binding of ribosomal protein L5, a natural partner of 5S rRNA, which demonstrates the functional adaptation of its structural mimicry. Since the exon-skipped splice product encodes full-length TFIIIA protein, these results reveal a ribosomal protein-mRNA interaction that is involved in 5S rRNA synthesis and has implications for cross-coordination of ribosomal components. This study also provides insight into the origin and function of a newfound class of structured RNA that regulates alternative splicing. PMID:19377483

A plant 5S ribosomal RNA mimic regulates alternative splicing of transcription factor IIIA pre-mRNAs.

PubMed

Hammond, Ming C; Wachter, Andreas; Breaker, Ronald R

2009-05-01

Transcription factor IIIA (TFIIIA) is required for eukaryotic synthesis of 5S ribosomal RNA by RNA polymerase III. Here we report the discovery of a structured RNA element with clear resemblance to 5S rRNA that is conserved within TFIIIA precursor mRNAs from diverse plant lineages. TFIIIA protein expression is controlled by alternative splicing of the exon containing the plant 5S rRNA mimic (P5SM). P5SM triggers exon skipping upon binding of ribosomal protein L5, a natural partner of 5S rRNA, which demonstrates the functional adaptation of its structural mimicry. As the exon-skipped splice product encodes full-length TFIIIA protein, these results reveal a ribosomal protein-mRNA interaction that is involved in 5S rRNA synthesis and has implications for cross-coordination of ribosomal components. This study also provides insight into the origin and function of a newfound class of structured RNA that regulates alternative splicing.

β-Amylase–Like Proteins Function as Transcription Factors in Arabidopsis, Controlling Shoot Growth and Development[C][W][OA

PubMed Central

Reinhold, Heike; Soyk, Sebastian; Šimková, Klára; Hostettler, Carmen; Marafino, John; Mainiero, Samantha; Vaughan, Cara K.; Monroe, Jonathan D.; Zeeman, Samuel C.

2011-01-01

Plants contain β-amylase–like proteins (BAMs; enzymes usually associated with starch breakdown) present in the nucleus rather than targeted to the chloroplast. They possess BRASSINAZOLE RESISTANT1 (BZR1)-type DNA binding domains—also found in transcription factors mediating brassinosteroid (BR) responses. The two Arabidopsis thaliana BZR1-BAM proteins (BAM7 and BAM8) bind a cis-regulatory element that both contains a G box and resembles a BR-responsive element. In protoplast transactivation assays, these BZR1-BAMs activate gene expression. Structural modeling suggests that the BAM domain’s glucan binding cleft is intact, but the recombinant proteins are at least 1000 times less active than chloroplastic β-amylases. Deregulation of BZR1-BAMs (the bam7bam8 double mutant and BAM8-overexpressing plants) causes altered leaf growth and development. Of the genes upregulated in plants overexpressing BAM8 and downregulated in bam7bam8 plants, many carry the cis-regulatory element in their promoters. Many genes that respond to BRs are inversely regulated by BZR1-BAMs. We propose a role for BZR1-BAMs in controlling plant growth and development through crosstalk with BR signaling. Furthermore, we speculate that BZR1-BAMs may transmit metabolic signals by binding a ligand in their BAM domain, although diurnal changes in the concentration of maltose, a candidate ligand produced by chloroplastic β-amylases, do not influence their transcription factor function. PMID:21487098

Migration of Older to New Digital Control Systems in Nuclear Power Plant Main Control Rooms

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kovesdi, Casey Robert; Joe, Jeffrey Clark

The United States (U.S.) Department of Energy (DOE) Office of Nuclear Energy (NE) has the primary mission to advance nuclear power by resolving socio-technical issues through research and development (R&D). One DOE-NE activity supporting this mission is the Light Water Reactor Sustainability (LWRS) program. LWRS has the overall objective to sustain the operation of existing commercial nuclear power plants (NPPs) through conducting R&D across multiple “pathways,” or R&D focus areas. The Advanced Instrumentation, Information, and Control (II&C) Systems Technologies pathway conducts targeted R&D to address aging and reliability concerns with the legacy instrumentation and control (I&C) and related information systemsmore » in operating U.S. NPPs. This work involves (1) ensuring that legacy analog II&C systems are not life-limiting issues for the LWR fleet, and (2) implementing digital II&C technology in a manner that enables broad innovation and business improvement in the NPP operating model. Under the LWRS Advanced II&C pathway, Human Factors experts at Idaho National Laboratory (INL) have been conducting R&D in support of NPP main control room (MCR) modernization activities. Work in prior years has focused on migrating analog I&C systems to new digital I&C systems (). In fiscal year 2016 (FY16), one new focus area for this research is migrating older digital I&C systems to new and advanced digital I&C systems. This report summarizes a plan for conducting a digital-to-digital migration of a legacy digital I&C system to a new digital I&C system in support of control room modernization activities.« less

In-plant control applications and their effect on treatability of a textile mill wastewater.

PubMed

Dulkadiroglu, H; Eremektar, G; Dogruel, S; Uner, H; Germirli-Babuna, F; Orhon, D

2002-01-01

Water minimization and exploration of the potential for wastewater recovery and reuse are priority issues of industrial wastewater management. They are extremely significant for the textile industry commonly characterized with a high water demand. The study presents a detailed in-plant control survey for a wool finishing plant. A comprehensive process profile and wastewater characterization indicate that process water consumption can be reduced by 34%, and 23% of the wastewater volume can be recovered for reuse. Treatability of reusable wastewater fraction and the effect of in-plant control applications on effluent treatability were also investigated.

Regulation of phosphate starvation responses in higher plants.

PubMed

Yang, Xiao Juan; Finnegan, Patrick M

2010-04-01

Phosphorus (P) is often a limiting mineral nutrient for plant growth. Many soils worldwide are deficient in soluble inorganic phosphate (P(i)), the form of P most readily absorbed and utilized by plants. A network of elaborate developmental and biochemical adaptations has evolved in plants to enhance P(i) acquisition and avoid starvation. Controlling the deployment of adaptations used by plants to avoid P(i) starvation requires a sophisticated sensing and regulatory system that can integrate external and internal information regarding P(i) availability. In this review, the current knowledge of the regulatory mechanisms that control P(i) starvation responses and the local and long-distance signals that may trigger P(i) starvation responses are discussed. Uncharacterized mutants that have P(i)-related phenotypes and their potential to give us additional insights into regulatory pathways and P(i) starvation-induced signalling are also highlighted and assessed. An impressive list of factors that regulate P(i) starvation responses is now available, as is a good deal of knowledge regarding the local and long-distance signals that allow a plant to sense and respond to P(i) availability. However, we are only beginning to understand how these factors and signals are integrated with one another in a regulatory web able to control the range of responses demonstrated by plants grown in low P(i) environments. Much more knowledge is needed in this agronomically important area before real gains can be made in improving P(i) acquisition in crop plants.

An Evolutionarily Conserved DOF-CONSTANS Module Controls Plant Photoperiodic Signaling.

PubMed

Lucas-Reina, Eva; Romero-Campero, Francisco J; Romero, José M; Valverde, Federico

2015-06-01

The response to daylength is a crucial process that evolved very early in plant evolution, entitling the early green eukaryote to predict seasonal variability and attune its physiological responses to the environment. The photoperiod responses evolved into the complex signaling pathways that govern the angiosperm floral transition today. The Chlamydomonas reinhardtii DNA-Binding with One Finger (CrDOF) gene controls transcription in a photoperiod-dependent manner, and its misexpression influences algal growth and viability. In short days, CrDOF enhances CrCO expression, a homolog of plant CONSTANS (CO), by direct binding to its promoter, while it reduces the expression of cell division genes in long days independently of CrCO. In Arabidopsis (Arabidopsis thaliana), transgenic plants overexpressing CrDOF show floral delay and reduced expression of the photoperiodic genes CO and FLOWERING LOCUS T. The conservation of the DOF-CO module during plant evolution could be an important clue to understanding diversification by the inheritance of conserved gene toolkits in key developmental programs. © 2015 American Society of Plant Biologists. All Rights Reserved.

Ethnoveterinary plants and practices used for ecto-parasite control in semi-arid smallholder farming areas of Zimbabwe.

PubMed

Nyahangare, Emmanuel Tendai; Mvumi, Brighton Marimanzi; Mutibvu, Tonderai

2015-04-30

The inclusion of traditional plant-based ecto-parasite control methods in primary health care of livestock is increasingly becoming an important intervention for improving livestock productivity in resource-challenged smallholder farming areas. In this study, commonly used plants used for the control of cattle ticks and other pests were identified through a survey in four semi-arid districts of Zimbabwe. A standard structured questionnaire with details of demographics, socioeconomic status of households, livestock parasites, control practices and list of ethnoveterinary plants used was used to interview 233 knowledgeable smallholder farmers in four districts. Focus group discussions with community members further provided insights on how the plants were being used and other issues surrounding ecto-parasite control and indigenous knowledge systems in the study areas. The older generation (>40 years) of the respondents were knowledgeable about ethnoveterinary plants and practices. Overall, 51 plant species were reportedly effective against cattle ticks and other livestock parasites. The most frequently mentioned plants were in descending order, Cissus quadrangularis (30.1%), Lippia javanica (19.6%), Psydrax livida (14.9%) and Aloe sp (14.9%). Most of the plant materials were prepared by crushing and soaking in water and spraying the extract on animals. Despite the knowledge of these useful pesticidal plants, the preferred animal health care for cattle and other highly ranked livestock species is still the use of commercial acaricides. Cattle dipping services were reported sporadic by 48% of the respondents. Traditional knowledge and plants are considered only as an alternative in the absence of conventional synthetic products. Livestock farming communities know of plant species used for livestock ecto-parasite control. The plant species are mostly used to complement commercial products. More work, is required to confirm the acaricidal properties claimed by the

The use of an electric field in increasing the resistance of plants to the action of unfavorable space flight factors

NASA Astrophysics Data System (ADS)

Nechitailo, G.; Gordeev, A.

The key role in increasing the resistance of plants to unfavorable space flight factors is assigned to biomembranes of root cells. It is these biomembranes on which numerous biochemical and biophysical processes determining the adaptive capacity of plant organisms occur. In the initial period of exposure to unfavorable space flight factors the adaptational reactions of the plant organism undoubtedly increase its resistance. But the intensification of removal of H+ ions through the plasmalemma with an increase of the external influence sharply raises the quantity of cations leaving the cell, which leads to the accumu lation of a considerable quantity of intracellular negative charges. These charges together with negative charges built in the membrane force protons to concentrate on the external surface of the membrane. Since protons have a very strong electric field, they form such a charge of which the electric field is about from several to hundreds of V/cm. The concentration of positive charges of protons entails the formation of a double electric field which extremely impedes the diffusion of other ions. Thus, a proton barrier is formed. Its length can be very considerable due to which the whole process of transmembrane energy and mass-transfer is disturbed. The proton barrier is easily destroyed by a weak electric field created in the root zone. In experiment on electrostimulation of different plants under space flight conditions at the orbital station MIR the absorption of nutrient elements by the root system increased to the optimal level, the ratio of physiologically active substances in the rhizosphere was normalized, the content of chlorophyll, carotin, and ascorbic acid in leaves corresponded to the ground-based control. Understanding of the mechanism of formation of a proton barrier on the plasmalemma of root cells as a result of the response of plants to the negative action of external factors (microgravity) is of great importance. It allows the

The use of an electric field in increasing the resistance of plants to the action of unfavorable space flight factors

NASA Astrophysics Data System (ADS)

Nechitailo, G.; Gordeev, A.

2004-01-01

The key role in increasing the resistance of plants to unfavorable space flight factors is assigned to biomembranes of root cells. It is these biomembranes in which numerous biochemical and biophysical processes determining the adaptive capacity of plant organisms occur. In the initial period of exposure to unfavorable space flight factors the adaptation reactions of the plant organism undoubtedly increase its resistance. But the intensification of removal of H + ions through the plasmalemma with an increase of the external influence sharply raises the quantity of cations leaving the cell, which leads to the accumulation of a considerable quantity of intracellular negative charges. These charges together with negative charges built in the membrane force protons to concentrate on the external surface of the membrane. Since protons have a very strong electric field, they form such a charge of which the electric field is about from several to hundreds of V/cm. The concentration of positive charges of protons entails the formation of a double electric field which extremely impedes the diffusion of other ions. Thus, a proton barrier is formed. Its length can be very considerable due to which the whole process of transmembrane energy and mass-transfer is disturbed. The proton barrier is easily destroyed by a weak electric field created in the root zone. In experiments on electrostimulation of different plants under space flight conditions at the orbital station MIR the absorption of nutrient elements by the root system increased to the optimal level, the ratio of physiologically active substances in the rhizosphere was normalized, the content of chlorophyll, carotin, and ascorbic acid in leaves corresponded to the ground-based control. Understanding of the mechanism of formation of a proton barrier on the plasmalemma of root cells as a result of the response of plants to the negative action of external factors (microgravity) is of great importance. It allows the

Virulence of plant pathogenic bacteria attenuated by degradation of fatty acid cell-to-cell signaling factors.

PubMed

Newman, Karyn L; Chatterjee, Subhadeep; Ho, Kimberly A; Lindow, Steven E

2008-03-01

Diffusible signal factor (DSF) is a fatty acid signal molecule involved in regulation of virulence in several Xanthomonas species as well as Xylella fastidiosa. In this study, we identified a variety of bacteria that could disrupt DSF-mediated induction of virulence factors in Xanthomonas campestris pv. campestris. While many bacteria had the ability to degrade DSF, several bacterial strains belonging to genera Bacillus, Paenibacillus, Microbacterium, Staphylococcus, and Pseudomonas were identified that were capable of particularly rapid degradation of DSF. The molecular determinants for rapid degradation of DSF in Pseudomonas spp. strain G were elucidated. Random transposon mutants of strain G lacking the ability to degrade DSF were isolated. Cloning and characterization of disrupted genes in these strains revealed that carAB, required for the synthesis of carbamoylphosphate, a precursor for pyrimidine and arginine biosynthesis is required for rapid degradation of DSF in strain G. Complementation of carAB mutants restored both pyrimidine prototrophy and DSF degradation ability of the strain G mutant. An Escherichia coli strain harboring carAB of Pseudomonas spp. strain G degrades DSF more rapidly than the parental strain, and overexpression of carAB in trans increased the ability of Pseudomonas spp. strain G to degrade as compared with the parental strain. Coinoculation of X. campestris pv. campestris with DSF-degrading bacteria into mustard and cabbage leaves reduced disease severity up to twofold compared with plants inoculated only with the pathogen. Likewise, disease incidence and severity in grape stems coinoculated with Xylella fastidiosa and DSF-degrading strains were significantly reduced compared with plants inoculated with the pathogen alone. Coinoculation of grape plants with a carAB mutant of Pseudomonas spp. strain G complemented with carAB in trans reduced disease severity as well or better than the parental strain. These results indicate that

Resource availability controls fungal diversity across a plant diversity gradient

USGS Publications Warehouse

Waldrop, M.P.; Zak, D.R.; Blackwood, C.B.; Curtis, C.D.; Tilman, D.

2006-01-01

Despite decades of research, the ecological determinants of microbial diversity remain poorly understood. Here, we test two alternative hypotheses concerning the factors regulating fungal diversity in soil. The first states that higher levels of plant detritus production increase the supply of limiting resources (i.e. organic substrates) thereby increasing fungal diversity. Alternatively, greater plant diversity increases the range of organic substrates entering soil, thereby increasing the number of niches to be filled by a greater array of heterotrophic fungi. These two hypotheses were simultaneously examined in experimental plant communities consisting of one to 16 species that have been maintained for a decade. We used ribosomal intergenic spacer analysis (RISA), in combination with cloning and sequencing, to quantify fungal community composition and diversity within the experimental plant communities. We used soil microbial biomass as a temporally integrated measure of resource supply. Plant diversity was unrelated to fungal diversity, but fungal diversity was a unimodal function of resource supply. Canonical correspondence analysis (CCA) indicated that plant diversity showed a relationship to fungal community composition, although the occurrence of RISA bands and operational taxonomic units (OTUs) did not differ among the treatments. The relationship between fungal diversity and resource availability parallels similar relationships reported for grasslands, tropical forests, coral reefs, and other biotic communities, strongly suggesting that the same underlying mechanisms determine the diversity of organisms at multiple scales. ?? 2006 Blackwell Publishing Ltd/CNRS.

Novel Genomic and Evolutionary Insight of WRKY Transcription Factors in Plant Lineage

PubMed Central

Mohanta, Tapan Kumar; Park, Yong-Hwan; Bae, Hanhong

2016-01-01

The evolutionarily conserved WRKY transcription factor (TF) regulates different aspects of gene expression in plants, and modulates growth, development, as well as biotic and abiotic stress responses. Therefore, understanding the details regarding WRKY TFs is very important. In this study, large-scale genomic analyses of the WRKY TF gene family from 43 plant species were conducted. The results of our study revealed that WRKY TFs could be grouped and specifically classified as those belonging to the monocot or dicot plant lineage. In this study, we identified several novel WRKY TFs. To our knowledge, this is the first report on a revised grouping system of the WRKY TF gene family in plants. The different forms of novel chimeric forms of WRKY TFs in the plant genome might play a crucial role in their evolution. Tissue-specific gene expression analyses in Glycine max and Phaseolus vulgaris showed that WRKY11-1, WRKY11-2 and WRKY11-3 were ubiquitously expressed in all tissue types, and WRKY15-2 was highly expressed in the stem, root, nodule and pod tissues in G. max and P. vulgaris. PMID:27853303

Novel Genomic and Evolutionary Insight of WRKY Transcription Factors in Plant Lineage.

PubMed

Mohanta, Tapan Kumar; Park, Yong-Hwan; Bae, Hanhong

2016-11-17

The evolutionarily conserved WRKY transcription factor (TF) regulates different aspects of gene expression in plants, and modulates growth, development, as well as biotic and abiotic stress responses. Therefore, understanding the details regarding WRKY TFs is very important. In this study, large-scale genomic analyses of the WRKY TF gene family from 43 plant species were conducted. The results of our study revealed that WRKY TFs could be grouped and specifically classified as those belonging to the monocot or dicot plant lineage. In this study, we identified several novel WRKY TFs. To our knowledge, this is the first report on a revised grouping system of the WRKY TF gene family in plants. The different forms of novel chimeric forms of WRKY TFs in the plant genome might play a crucial role in their evolution. Tissue-specific gene expression analyses in Glycine max and Phaseolus vulgaris showed that WRKY11-1, WRKY11-2 and WRKY11-3 were ubiquitously expressed in all tissue types, and WRKY15-2 was highly expressed in the stem, root, nodule and pod tissues in G. max and P. vulgaris.

Matematical modeling of galophytic plants productivity taking into account the temperature factor and soil salinity level

NASA Astrophysics Data System (ADS)

Natalia, Slyusar; Pisman, Tamara; Pechurkin, Nikolai S.

Among the most challenging tasks faced by contemporary ecology is modeling of biological production process in different plant communities. The difficulty of the task is determined by the complexity of the study material. Models showing the influence of climate and climate change on plant growth, which would also involve soil site parameters, could be of both practical and theoretical interest. In this work a mathematical model has been constructed to describe the growth dynamics of different plant communities of halophytic meadows as dependent upon the temperature factor and soil salinity level, which could be further used to predict yields of these plant communities. The study was performed on plants of halophytic meadows in the coastal area of Lake of the Republic of Khakasia in 2004 - 2006. Every plant community grew on the soil of a different level of salinity - the amount of the solid residue of the saline soil aqueous extract. The mathematical model was analyzed using field data of 2004 and 2006, the years of contrasting air temperatures. Results of model investigations show that there is a correlation between plant growth and the temperature of the air for plant communities growing on soils containing the lowest (0.1Thus, results of our study, in which we used a mathematical model describing the development of plant communities of halophytic meadows and field measurements, suggest that both climate conditions (temperature) and ecological factors of the plants' habitat (soil salinity level) should be taken into account when constructing models for predicting crop yields.

Climate and soil factors influencing seedling recruitment of plant species used for dryland restoration

NASA Astrophysics Data System (ADS)

Muñoz-Rojas, Miriam; Erickson, Todd E.; Martini, Dylan C.; Dixon, Kingsley W.; Merritt, David J.

2016-06-01

Land degradation affects 10-20 % of drylands globally. Intensive land use and management, large-scale disturbances such as extractive operations, and global climate change, have contributed to degradation of these systems worldwide. Restoring these damaged environments is critical to improving ecosystem services and functions, conserve biodiversity, and contribute to climate resilience, food security, and landscape sustainability. Here, we present a case study on plant species of the mining intensive semi-arid Pilbara region in Western Australia that examines the effects of climate and soil factors on the restoration of drylands. We analysed the effects of a range of rainfall and temperature scenarios and the use of alternative soil materials on seedling recruitment of key native plant species from this area. Experimental studies were conducted in controlled environment facilities where conditions simulated those found in the Pilbara. Soil from topsoil (T) stockpiles and waste materials (W) from an active mine site were mixed at different proportions (100 % T, 100 % W, and two mixes of topsoil and waste at 50 : 50 and 25 : 75 ratios) and used as growth media. Our results showed that seedling recruitment was highly dependent on soil moisture and emergence was generally higher in the topsoil, which had the highest available water content. In general, responses to the climate scenarios differed significantly among the native species which suggest that future climate scenarios of increasing drought might affect not only seedling recruitment but also diversity and structure of native plant communities. The use of waste materials from mining operations as growth media could be an alternative to the limited topsoil. However, in the early stages of plant establishment successful seedling recruitment can be challenging in the absence of water. These limitations could be overcome by using soil amendments but the cost associated to these solutions at large landscape scales

The trend of digital control system design for nuclear power plants in Korea

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, S. H.; Jung, H. Y.; Yang, C. Y.

2006-07-01

Currently there are 20 nuclear power plants (NPPs) in operation, and 6 more units are under construction in Korea. The control systems of those NPPs have also been developed together with the technology advancement. Control systems started with On-Off control using the relay logic, had been evolved into Solid-State logic using TTL ICs, and applied with the micro-processors since the Yonggwang NPP Units 3 and 4 which started its construction in 1989. Multiplexers are also installed at the local plant areas to collect field input and to send output signals while communicating with the controllers located in the system cabinetsmore » near the main control room in order to reduce the field wiring cables. The design of the digital control system technology for the NPPs in Korea has been optimized to maximize the operability as well as the safety through the design, construction, start-up and operation experiences. Both Shin-Kori Units 1 and 2 and Shin-Wolsong Units 1 and 2 NPP projects under construction are being progressed at the same time. Digital Plant Control Systems of these projects have adopted multi-loop controllers, redundant loop configuration, and soft control system for the radwaste system. Programmable Logic Controller (PLC) and Distributed Control System (DCS) are applied with soft control system in Shin-Kori Units 3 and 4. This paper describes the evolvement of control system at the NPPs in Korea and the experience and design improvement through the observation of the latest failure of the digital control system. In addition, design concept and its trend of the digital control system being applied to the NPP in Korea are introduced. (authors)« less

Identification of Fungal Colonies on Ground Control and Flight Veggie Plant Pillows

NASA Technical Reports Server (NTRS)

Scotten, Jessica E.; Hummerick, Mary E.; Khodadad, Christina L.; Spencer, Lashelle E.; Massa, Gioia D.

2017-01-01

The Veggie system focuses on growing fresh produce that can be harvested and consumed by astronauts. The microbial colonies in each Veggie experiment are evaluated to determine the safety level of the produce and then differences between flight and ground samples. The identifications of the microbial species can detail risks or benefits to astronaut and plant health. Each Veggie ground or flight experiment includes six plants grown from seeds that are glued into wicks in Teflon pillows filled with clay arcillite and fertilizer. Fungal colonies were isolated from seed wicks, growth media, and lettuce (cv. 'Outredgeous') roots grown in VEG-01B pillows on ISS and in corresponding ground control pillows grown in controlled growth chambers. The colonies were sorted by morphology and identified using MicroSeq(TM) 500 16s rDNA Bacterial Identification System and BIOLOG GEN III MicroPlate(TM). Health risks for each fungal identification were then assessed using literature sources. The goal was to identify all the colonies isolated from flight and ground control VEG-01B plants, roots, and rooting medium and compare the resulting identifications.

A broad-spectrum, efficient and nontransgenic approach to control plant viruses by application of salicylic acid and jasmonic acid.

PubMed

Shang, Jing; Xi, De-Hui; Xu, Fei; Wang, Shao-Dong; Cao, Sen; Xu, Mo-Yun; Zhao, Ping-Ping; Wang, Jian-Hui; Jia, Shu-Dan; Zhang, Zhong-Wei; Yuan, Shu; Lin, Hong-Hui

2011-02-01

Plant viruses cause many diseases that lead to significant economic losses. However, most of the approaches to control plant viruses, including transgenic processes or drugs are plant-species-limited or virus-species-limited, and not very effective. We introduce an application of jasmonic acid (JA) and salicylic acid (SA), a broad-spectrum, efficient and nontransgenic method, to improve plant resistance to RNA viruses. Applying 0.06 mM JA and then 0.1 mM SA 24 h later, enhanced resistance to Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Turnip crinkle virus (TCV) in Arabidopsis, tobacco, tomato and hot pepper. The inhibition efficiency to virus replication usually achieved up to 80-90%. The putative molecular mechanism was investigated. Some possible factors affecting the synergism of JA and SA have been defined, including WRKY53, WRKY70, PDF1.2, MPK4, MPK2, MPK3, MPK5, MPK12, MPK14, MKK1, MKK2, and MKK6. All genes involving in the synergism of JA and SA were investigated. This approach is safe to human beings and environmentally friendly and shows potential as a strong tool for crop protection against plant viruses.

Comprehensive Genome-Wide Classification Reveals That Many Plant-Specific Transcription Factors Evolved in Streptophyte Algae

PubMed Central

Wilhelmsson, Per K I; Mühlich, Cornelia; Ullrich, Kristian K

2017-01-01

Abstract Plant genomes encode many lineage-specific, unique transcription factors. Expansion of such gene families has been previously found to coincide with the evolution of morphological complexity, although comparative analyses have been hampered by severe sampling bias. Here, we make use of the recently increased availability of plant genomes. We have updated and expanded previous rule sets for domain-based classification of transcription associated proteins (TAPs), comprising transcription factors and transcriptional regulators. The genome-wide annotation of these protein families has been analyzed and made available via the novel TAPscan web interface. We find that many TAP families previously thought to be specific for land plants actually evolved in streptophyte (charophyte) algae; 26 out of 36 TAP family gains are inferred to have occurred in the common ancestor of the Streptophyta (uniting the land plants—Embryophyta—with their closest algal relatives). In contrast, expansions of TAP families were found to occur throughout streptophyte evolution. 17 out of 76 expansion events were found to be common to all land plants and thus probably evolved concomitant with the water-to-land-transition. PMID:29216360

From Genes to Networks: Characterizing Gene-Regulatory Interactions in Plants.

PubMed

Kaufmann, Kerstin; Chen, Dijun

2017-01-01

Plants, like other eukaryotes, have evolved complex mechanisms to coordinate gene expression during development, environmental response, and cellular homeostasis. Transcription factors (TFs), accompanied by basic cofactors and posttranscriptional regulators, are key players in gene-regulatory networks (GRNs). The coordinated control of gene activity is achieved by the interplay of these factors and by physical interactions between TFs and DNA. Here, we will briefly outline recent technological progress made to elucidate GRNs in plants. We will focus on techniques that allow us to characterize physical interactions in GRNs in plants and to analyze their regulatory consequences. Targeted manipulation allows us to test the relevance of specific gene-regulatory interactions. The combination of genome-wide experimental approaches with mathematical modeling allows us to get deeper insights into key-regulatory interactions and combinatorial control of important processes in plants.

Motor power factor controller with a reduced voltage starter

NASA Technical Reports Server (NTRS)

Nola, F. J. (Inventor)

1981-01-01

A power factor type motor controller is disclosed in which the conventional power factor constant voltage command signal is replaced during a starting interval with a graduated control voltage. This continuation-impart of a pending patent application (Serial No. 199, 765: Three Phase Factor Controller) provides a means for modifying the operation of the system for a motor start-up interval of 5 to 30 second. Using a ramp generators, an initial ramp-like signal replaces a constant power factor signal supplied by a potentiometer. The ramp-like signal is applied to a 15 terminal where it is summed with an operating power factor signal from phase detectors in order to obtain a control signal for ultimately controlling SCR devices. The SCR devices are turned on at an advancing rate with time responsive to the combination signal described rather than simply a function of a ramp-like signal alone.

RNA-Seq effectively monitors gene expression in Eutrema salsugineum plants growing in an extreme natural habitat and in controlled growth cabinet conditions

PubMed Central

2013-01-01

Background The investigation of extremophile plant species growing in their natural environment offers certain advantages, chiefly that plants adapted to severe habitats have a repertoire of stress tolerance genes that are regulated to maximize plant performance under physiologically challenging conditions. Accordingly, transcriptome sequencing offers a powerful approach to address questions concerning the influence of natural habitat on the physiology of an organism. We used RNA sequencing of Eutrema salsugineum, an extremophile relative of Arabidopsis thaliana, to investigate the extent to which genetic variation and controlled versus natural environments contribute to differences between transcript profiles. Results Using 10 million cDNA reads, we compared transcriptomes from two natural Eutrema accessions (originating from Yukon Territory, Canada and Shandong Province, China) grown under controlled conditions in cabinets and those from Yukon plants collected at a Yukon field site. We assessed the genetic heterogeneity between individuals using single-nucleotide polymorphisms (SNPs) and the expression patterns of 27,016 genes. Over 39,000 SNPs distinguish the Yukon from the Shandong accessions but only 4,475 SNPs differentiated transcriptomes of Yukon field plants from an inbred Yukon line. We found 2,989 genes that were differentially expressed between the three sample groups and multivariate statistical analyses showed that transcriptomes of individual plants from a Yukon field site were as reproducible as those from inbred plants grown under controlled conditions. Predicted functions based upon gene ontology classifications show that the transcriptomes of field plants were enriched by the differential expression of light- and stress-related genes, an observation consistent with the habitat where the plants were found. Conclusion Our expectation that comparative RNA-Seq analysis of transcriptomes from plants originating in natural habitats would be confounded

Emerging microbial biocontrol strategies for plant pathogens.

PubMed

Syed Ab Rahman, Sharifah Farhana; Singh, Eugenie; Pieterse, Corné M J; Schenk, Peer M

2018-02-01

To address food security, agricultural yields must increase to match the growing human population in the near future. There is now a strong push to develop low-input and more sustainable agricultural practices that include alternatives to chemicals for controlling pests and diseases, a major factor of heavy losses in agricultural production. Based on the adverse effects of some chemicals on human health, the environment and living organisms, researchers are focusing on potential biological control microbes as viable alternatives for the management of pests and plant pathogens. There is a growing body of evidence that demonstrates the potential of leaf and root-associated microbiomes to increase plant efficiency and yield in cropping systems. It is important to understand the role of these microbes in promoting growth and controlling diseases, and their application as biofertilizers and biopesticides whose success in the field is still inconsistent. This review focusses on how biocontrol microbes modulate plant defense mechanisms, deploy biocontrol actions in plants and offer new strategies to control plant pathogens. Apart from simply applying individual biocontrol microbes, there are now efforts to improve, facilitate and maintain long-term plant colonization. In particular, great hopes are associated with the new approaches of using "plant-optimized microbiomes" (microbiome engineering) and establishing the genetic basis of beneficial plant-microbe interactions to enable breeding of "microbe-optimized crops". Copyright © 2017 Elsevier B.V. All rights reserved.

A Physiological Neural Controller of a Muscle Fiber Oculomotor Plant in Horizontal Monkey Saccades

PubMed Central

Enderle, John D.

2014-01-01

A neural network model of biophysical neurons in the midbrain is presented to drive a muscle fiber oculomotor plant during horizontal monkey saccades. Neural circuitry, including omnipause neuron, premotor excitatory and inhibitory burst neurons, long lead burst neuron, tonic neuron, interneuron, abducens nucleus, and oculomotor nucleus, is developed to examine saccade dynamics. The time-optimal control strategy by realization of agonist and antagonist controller models is investigated. In consequence, each agonist muscle fiber is stimulated by an agonist neuron, while an antagonist muscle fiber is unstimulated by a pause and step from the antagonist neuron. It is concluded that the neural network is constrained by a minimum duration of the agonist pulse and that the most dominant factor in determining the saccade magnitude is the number of active neurons for the small saccades. For the large saccades, however, the duration of agonist burst firing significantly affects the control of saccades. The proposed saccadic circuitry establishes a complete model of saccade generation since it not only includes the neural circuits at both the premotor and motor stages of the saccade generator, but also uses a time-optimal controller to yield the desired saccade magnitude. PMID:24944832

Herbal Remedies for Coccidiosis Control: A Review of Plants, Compounds, and Anticoccidial Actions

PubMed Central

Muthamilselvan, Thangarasu; Wu, Yueh-Chen

2016-01-01

Coccidiosis is the bane of the poultry industry causing considerable economic loss. Eimeria species are known as protozoan parasites to cause morbidity and death in poultry. In addition to anticoccidial chemicals and vaccines, natural products are emerging as an alternative and complementary way to control avian coccidiosis. In this review, we update recent advances in the use of anticoccidial phytoextracts and phytocompounds, which cover 32 plants and 40 phytocompounds, following a database search in PubMed, Web of Science, and Google Scholar. Four plant products commercially available for coccidiosis are included and discussed. We also highlight the chemical and biological properties of the plants and compounds as related to coccidiosis control. Emphasis is placed on the modes of action of the anticoccidial plants and compounds such as interference with the life cycle of Eimeria, regulation of host immunity to Eimeria, growth regulation of gut bacteria, and/or multiple mechanisms. Biological actions, mechanisms, and prophylactic/therapeutic potential of the compounds and extracts of plant origin in coccidiosis are summarized and discussed. PMID:27429634

Characterizing photosynthesis and transpiration of plant communities in controlled environments

NASA Technical Reports Server (NTRS)

Monje, O.; Bugbee, B.

1996-01-01

CO2 and water vapor fluxes of hydroponically grown wheat and soybean canopies were measured continuously in several environments with an open gas exchange system. Canopy CO2 fluxes reflect the photosynthetic efficiency of a plant community, and provide a record of plant growth and health. There were significant diurnal fluctuations in root and shoot CO2 fluxes, and in shoot water vapor fluxes. Canopy stomatal conductance (Gc) to water vapor was calculated from simultaneous measurements of canopy temperature (Tcan) and transpiration rates (Tr). Tr in the dark was substantial, and there were large diurnal fluctuations in both Gc and Tr. Canopy net Photosynthesis (Pnet), Tr, and Gc increased with increasing net radiation. Gc increased with Tr, suggesting that the stomata of plants in controlled environments (CEs) behave differently from field-grown plants. A transpiration model based on measurements of Gc was developed for CEs. The model accurately predicted Tr from a soybean canopy.

A pair of light signaling factors FHY3 and FAR1 regulates plant immunity by modulating chlorophyll biosynthesis.

PubMed

Wang, Wanqing; Tang, Weijiang; Ma, Tingting; Niu, De; Jin, Jing Bo; Wang, Haiyang; Lin, Rongcheng

2016-01-01

Light and chloroplast function is known to affect the plant immune response; however, the underlying mechanism remains elusive. We previously demonstrated that two light signaling factors, FAR-RED ELONGATED HYPOCOTYL 3 (FHY3) and FAR-RED IMPAIRED RESPONSE 1 (FAR1), regulate chlorophyll biosynthesis and seedling growth via controlling HEMB1 expression in Arabidopsis thaliana. In this study, we reveal that FHY3 and FAR1 are involved in modulating plant immunity. We showed that the fhy3 far1 double null mutant displayed high levels of reactive oxygen species and salicylic acid (SA) and increased resistance to Pseudomonas syringae pathogen infection. Microarray analysis revealed that a large proportion of pathogen-related genes, particularly genes encoding nucleotide-binding and leucine-rich repeat domain resistant proteins, are highly induced in fhy3 far1. Genetic studies indicated that the defects of fhy3 far1 can be largely rescued by reducing SA signaling or blocking SA accumulation, and by overexpression of HEMB1, which encodes a 5-aminolevulinic acid dehydratase in the chlorophyll biosynthetic pathway. Furthermore, we found that transgenic plants with reduced expression of HEMB1 exhibit a phenotype similar to fhy3 far1. Taken together, this study demonstrates an important role of FHY3 and FAR1 in regulating plant immunity, through integrating chlorophyll biosynthesis and the SA signaling pathway. © The Authors. Journal of Integrative Plant Biology published by Wiley Publishing Asia Pty Ltd on behalf of Institute of Botany, Chinese Academy of Sciences.

Output-Feedback Model Predictive Control of a Pasteurization Pilot Plant based on an LPV model

NASA Astrophysics Data System (ADS)

Karimi Pour, Fatemeh; Ocampo-Martinez, Carlos; Puig, Vicenç

2017-01-01

This paper presents a model predictive control (MPC) of a pasteurization pilot plant based on an LPV model. Since not all the states are measured, an observer is also designed, which allows implementing an output-feedback MPC scheme. However, the model of the plant is not completely observable when augmented with the disturbance models. In order to solve this problem, the following strategies are used: (i) the whole system is decoupled into two subsystems, (ii) an inner state-feedback controller is implemented into the MPC control scheme. A real-time example based on the pasteurization pilot plant is simulated as a case study for testing the behavior of the approaches.

The Role of Plant Abiotic Factors on the Interactions Between Cnaphalocrocis medinalis (Lepidoptera: Crambidae) and its Host Plant.

PubMed

Tu, Kun-Yu; Tsai, Shin-Fu; Guo, Tzu-Wei; Lin, Hou-Ho; Yang, Zhi-Wei; Liao, Chung-Ta; Chuang, Wen-Po

2018-05-12

Atmospheric temperature increases along with increasing atmospheric CO2 concentration. This is a major concern for agroecosystems. Although the impact of an elevated temperature or increased CO2 has been widely reported, there are few studies investigating the combined effect of these two environmental factors on plant-insect interactions. In this study, plant responses (phenological traits, defensive enzyme activity, secondary compounds, defense-related gene expression and phytohormone) of Cnaphalocrocis medinalis (Guenée) (Lepidoptera: Pyralidae) -susceptible and resistant rice under various conditions (environment, soil type, variety, C. medinalis infestation) were used to examine the rice-C. medinalis interaction. The results showed that leaf chlorophyll content and trichome density in rice were variety-dependent. Plant defensive enzyme activities were affected environment, variety, or C. medinalis infestation. In addition, total phenolic content of rice leaves was decreased by elevated CO2 and temperature and C. medinalis infestation. Defense-related gene expression patterns were affected by environment, soil type, or C. medinalis infestation. Abscisic acid and salicylic acid content were decreased by C. medinalis infestation. However, jasmonic acid content was increased by C. medinalis infestation. Furthermore, under elevated CO2 and temperature, rice plants had higher abscisic acid content than plants under ambient conditions. The adult morphological traits of C. medinalis also were affected by environment. Under elevated CO2 and temperature, C. medinalis adults had greater body length in the second and third generations. Taken together these results indicated that elevated CO2 and temperature not only affects plants but also the specialized insects that feed on them.

Robust control of speed and temperature in a power plant gas turbine.

PubMed

Najimi, Ebrahim; Ramezani, Mohammad Hossein

2012-03-01

In this paper, an H(∞) robust controller has been designed for an identified model of MONTAZER GHAEM power plant gas turbine (GE9001E). In design phase, a linear model (ARX model) which is obtained using real data has been applied. Since the turbine has been used in a combined cycle power plant, its speed and also the exhaust gas temperature should be adjusted simultaneously by controlling fuel signals and compressor inlet guide vane (IGV) position. Considering the limitations on the system inputs, the aim of the control is to maintain the turbine speed and the exhaust gas temperature within desired interval under uncertainties and load demand disturbances. Simulation results of applying the proposed robust controller on the nonlinear model of the system (NARX model), fairly fulfilled the predefined aims. Simulations also show the improvement in the performance compared to MPC and PID controllers for the same conditions. Copyright © 2011 ISA. Published by Elsevier Ltd. All rights reserved.

[Mercury Distribution Characteristics and Atmospheric Mercury Emission Factors of Typical Waste Incineration Plants in Chongqing].

PubMed

Duan, Zhen-ya; Su, Hai-tao; Wang, Feng-yang; Zhang, Lei; Wang, Shu-xiao; Yu, Bin

2016-02-15

Waste incineration is one of the important atmospheric mercury emission sources. The aim of this article is to explore the atmospheric mercury pollution level of waste incineration industry from Chongqing. This study investigated the mercury emissions from a municipal solid waste incineration plant and a medical waste incineration plant in Chongqing. The exhaust gas samples in these two incineration plants were obtained using USA EPA 30B method. The mercury concentrations in the fly ash and bottom ash samples were analyzed. The results indicated that the mercury concentrations of the municipal solid waste and medical waste incineration plant in Chongqing were (26.4 +/- 22.7) microg x m(-3) and (3.1 +/- 0.8) microg x m(-3) in exhaust gas respectively, (5279.2 +/- 798.0) microg x kg(-1) and (11,709.5 +/- 460.5) microg x kg(-1) in fly ash respectively. Besides, the distribution proportions of the mercury content from municipal solid waste and medical waste in exhaust gas, fly ash, and bottom ash were 34.0%, 65.3%, 0.7% and 32.3%, 67.5%, 0.2% respectively; The mercury removal efficiencies of municipal solid waste and medical waste incineration plants were 66.0% and 67.7% respectively. The atmospheric mercury emission factors of municipal solid waste and medical waste incineration plants were (126.7 +/- 109.0) microg x kg(-1) and (46.5 +/- 12.0) microg x kg(-1) respectively. Compared with domestic municipal solid waste incineration plants in the Pearl River Delta region, the atmospheric mercury emission factor of municipal solid waste incineration plant in Chongqing was lower.