34 CFR 642.11 - What activities does the Secretary assist?

Code of Federal Regulations, 2011 CFR

2011-07-01

... funded under the Federal TRIO programs. (4) The use of appropriate educational technology in the... disabilities, students who are homeless children and youths, students who are foster care youth, or other...

34 CFR 642.40 - Allowable costs.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Transportation costs for participants and training staff. (g) Lodging and subsistence costs for participants and training staff. (h) Transportation costs, lodging and subsistence costs and fees for consultants, if any..., DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS What Conditions Must Be Met by a Grantee...

75 FR 37415 - Office of Postsecondary Education; Overview Information; Training Program for Federal TRIO...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-29

... literacy of students, including topics such as basic personal income, household money management and.... Priority 2. Training on: budget management, and the legislative and regulatory requirements for operation...

34 CFR 642.20 - How does the Secretary evaluate an application for a new award?

Code of Federal Regulations, 2011 CFR

2011-07-01

... (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO..., for an applicant who is conducting a Training program in the fiscal year immediately prior to the... during the first project year of that expiring Training program grant. (c) The Secretary selects...

34 CFR 642.25 - What is the review process for unsuccessful applicants?

Code of Federal Regulations, 2011 CFR

2011-07-01

...) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS How... was not evaluated during the competition may request that the Secretary review the application if— (i... notice inviting applications and the other published application materials for the competition; and (ii...

77 FR 28360 - Applications for New Awards; Training Program for Federal TRIO Programs

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-14

... economic literacy of students, including topics such as basic personal finance information, household money... of time, staff, money, or other resources while improving student learning or other educational... license that permits their free use or repurposing by others. Persistently lowest-achieving schools means...

34 CFR 642.5 - What regulations apply?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 3 2011-07-01 2011-07-01 false What regulations apply? 642.5 Section 642.5 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS General § 642.5 What regulations apply...

34 CFR 642.30 - What are allowable costs?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 3 2011-07-01 2011-07-01 false What are allowable costs? 642.30 Section 642.30... EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS What Conditions Must Be Met by a Grantee? § 642.30 What are allowable costs? Allowable project costs may include the following...

34 CFR 642.31 - What are unallowable costs?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 3 2011-07-01 2011-07-01 false What are unallowable costs? 642.31 Section 642.31... EDUCATION, DEPARTMENT OF EDUCATION TRAINING PROGRAM FOR FEDERAL TRIO PROGRAMS What Conditions Must Be Met by a Grantee? § 642.31 What are unallowable costs? Costs that may not be charged against a grant under...

34 CFR 645.21 - What assurances must an applicant include in an application?

Code of Federal Regulations, 2014 CFR

2014-07-01

... the project's participants will be low-income individuals who are potential first-generation college... participation in a project because the student would enter the project after the 9th grade; and (4) The project will collaborate with other Federal TRIO projects, GEAR UP projects, or programs serving similar...

34 CFR 645.21 - What assurances must an applicant include in an application?

Code of Federal Regulations, 2012 CFR

2012-07-01

... the project's participants will be low-income individuals who are potential first-generation college... participation in a project because the student would enter the project after the 9th grade; and (4) The project will collaborate with other Federal TRIO projects, GEAR UP projects, or programs serving similar...

34 CFR 645.21 - What assurances must an applicant include in an application?

Code of Federal Regulations, 2013 CFR

2013-07-01

... the project's participants will be low-income individuals who are potential first-generation college... participation in a project because the student would enter the project after the 9th grade; and (4) The project will collaborate with other Federal TRIO projects, GEAR UP projects, or programs serving similar...

34 CFR 642.6 - What definitions apply?

Code of Federal Regulations, 2011 CFR

2011-07-01

... projects funded under the Federal TRIO programs. Veteran means a person who— (1) Served on active duty as a... released under conditions other than dishonorable; (2) Served on active duty as a member of the Armed...) Was a member of a reserve component of the Armed Forces of the United States and was called to active...

Program Evaluation of Career Services Delivered through TRIO Student Support Services

ERIC Educational Resources Information Center

Spencer, Mary Louise

2014-01-01

This study was an evaluation of the career services component of TRIO Student Support Services at a Midwestern university. The problem in the local setting was the absence of empirical evidence demonstrating compliance of TRIO career services with the National Association of Colleges and Employers (NACE) Professional Standards for College and…

77 FR 76012 - Agency Information Collection Activities; Comment Request; Study of Implementation and Outcomes...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-26

... DEPARTMENT OF EDUCATION [Docket No. ED-2012-ICCD-0071] Agency Information Collection Activities; Comment Request; Study of Implementation and Outcomes in Upward Bound and Other TRIO Programs AGENCY... of Collection: Study of Implementation and Outcomes in Upward Bound and other TRIO Programs. OMB...

Index of Human Well-Being for the U.S.: A TRIO Approach

EPA Science Inventory

In 2011, the US Environmental Protection Agency’s (EPA) Office of Research and Development program, Sustainable and Healthy Communities, coined the term TRIO (Total Resources Impact Outcome) to represent approaches that fully incorporate all three pillars of community susta...

An Index of Human Well-being for the U.S.: A TRIO Approach

EPA Science Inventory

: In 2011, EPA’s Office of Research and Development program, Sustainable and Healthy Communities, coined the term TRIO (Total Resources Impact Outcome) to represent approaches the fully incorporate all three pillars of community sustainability – environmental, economic and social...

Behind their Cosmonaut Hotel crew quarters in Baikonur, Kazakhstan, Expedition 46-47 crewmember Tim Kopra of NASA (left), Yuri Malenchenko of the Russian Federal Space Agency (Roscosmos, center) and Tim Peake of the European Space Agency (right) pose for pictures Dec. 9 after a traditional tree-planting ceremony. The trio will launch Dec. 15 on their Soyuz TMA-19M spacecraft for a six-month mission on the International Space Station...NASA/Victor Zelentsov.

NASA Image and Video Library

2015-12-09

Behind their Cosmonaut Hotel crew quarters in Baikonur, Kazakhstan, Expedition 46-47 crewmember Tim Kopra of NASA (left), Yuri Malenchenko of the Russian Federal Space Agency (Roscosmos, center) and Tim Peake of the European Space Agency (right) pose for pictures Dec. 9 after a traditional tree-planting ceremony. The trio will launch Dec. 15 on their Soyuz TMA-19M spacecraft for a six-month mission on the International Space Station. NASA/Victor Zelentsov

The Impact of TRIO Student Support Services at a Midwestern Institution

ERIC Educational Resources Information Center

Wilson, TaJuan RaKeem

2015-01-01

First-generation, low-income, and disabled college students are an increasing population (Tinto, 2012). TRIO Student Support Services (SSS) is an academic support program funded by the U.S. Department of Education that seeks to support this demographic to overcome challenges and thrive while in college (Coffman, 2011). Utilizing a mixed methods…

Using Constructivist Career Development to Improve Career Decision Self-Efficacy in TRiO Students

ERIC Educational Resources Information Center

Grier-Reed, Tabitha; Ganuza, Zoila

2012-01-01

Although more high school graduates are attending college, many are not graduating (The Bill and Melinda Gates Foundation, 2004). First-generation, low-income, and underrepresented students are especially at risk for falling through the cracks. To help address this issue, programs such as TRiO Student Support Services (SSS) assist…

Behind the Cosmonaut Hotel crew quarters in Baikonur, Kazakhstan, Expedition 46-47 crewmember Tim Kopra of NASA (left) plants a tree at a site bearing his name Dec. 9 in a traditional pre-launch ceremony. Looking on are his crewmates, Tim Peake of the European Space Agency (center) and Yuri Malenchenko of the Russian Federal Space Agency (Roscosmos, right). The trio will launch Dec. 15 on their Soyuz TMA-19M spacecraft for a six-month mission on the International Space Station...NASA/Victor Zelentsov.

NASA Image and Video Library

2015-12-09

Behind the Cosmonaut Hotel crew quarters in Baikonur, Kazakhstan, Expedition 46-47 crewmember Tim Kopra of NASA (left) plants a tree at a site bearing his name Dec. 9 in a traditional pre-launch ceremony. Looking on are his crewmates, Tim Peake of the European Space Agency (center) and Yuri Malenchenko of the Russian Federal Space Agency (Roscosmos, right). The trio will launch Dec. 15 on their Soyuz TMA-19M spacecraft for a six-month mission on the International Space Station. NASA/Victor Zelentsov

Behind the Cosmonaut Hotel crew quarters in Baikonur, Kazakhstan, Expedition 46-47 crewmember Tim Peake of the European Space Agency (center) plants a tree at a site bearing his name Dec. 9 in a traditional pre-launch ceremony. Looking on are his crewmates, Yuri Malenchenko of the Russian Federal Space Agency (Roscosmos, left, and Tim Kopra of NASA (right). The trio will launch Dec. 15 on their Soyuz TMA-19M spacecraft for a six-month mission on the International Space Station...NASA/Victor Zelentsov.

NASA Image and Video Library

2015-12-09

Behind the Cosmonaut Hotel crew quarters in Baikonur, Kazakhstan, Expedition 46-47 crewmember Tim Peake of the European Space Agency (center) plants a tree at a site bearing his name Dec. 9 in a traditional pre-launch ceremony. Looking on are his crewmates, Yuri Malenchenko of the Russian Federal Space Agency (Roscosmos, left, and Tim Kopra of NASA (right). The trio will launch Dec. 15 on their Soyuz TMA-19M spacecraft for a six-month mission on the International Space Station. NASA/Victor Zelentsov

2020 Vision: Experts Forecast What the Digital Revolution Will Bring Next

ERIC Educational Resources Information Center

T.H.E. Journal, 2010

2010-01-01

In this article, a trio of current and former federal ed tech honchos, as well as a host of technology leaders nationwide, offer their forecasts, while also considering the lessons the first chapters of digital age have left behind. A timeline of learning technologies is also included.

A Unified Approach to Genotype Imputation and Haplotype-Phase Inference for Large Data Sets of Trios and Unrelated Individuals

PubMed Central

Browning, Brian L.; Browning, Sharon R.

2009-01-01

We present methods for imputing data for ungenotyped markers and for inferring haplotype phase in large data sets of unrelated individuals and parent-offspring trios. Our methods make use of known haplotype phase when it is available, and our methods are computationally efficient so that the full information in large reference panels with thousands of individuals is utilized. We demonstrate that substantial gains in imputation accuracy accrue with increasingly large reference panel sizes, particularly when imputing low-frequency variants, and that unphased reference panels can provide highly accurate genotype imputation. We place our methodology in a unified framework that enables the simultaneous use of unphased and phased data from trios and unrelated individuals in a single analysis. For unrelated individuals, our imputation methods produce well-calibrated posterior genotype probabilities and highly accurate allele-frequency estimates. For trios, our haplotype-inference method is four orders of magnitude faster than the gold-standard PHASE program and has excellent accuracy. Our methods enable genotype imputation to be performed with unphased trio or unrelated reference panels, thus accounting for haplotype-phase uncertainty in the reference panel. We present a useful measure of imputation accuracy, allelic R2, and show that this measure can be estimated accurately from posterior genotype probabilities. Our methods are implemented in version 3.0 of the BEAGLE software package. PMID:19200528

General parity between trio and pairwise breeding of laboratory mice in static caging.

PubMed

Kedl, Ross M; Wysocki, Lawrence J; Janssen, William J; Born, Willi K; Rosenbaum, Matthew D; Granowski, Julia; Kench, Jennifer A; Fong, Derek L; Switzer, Lisa A; Cruse, Margaret; Huang, Hua; Jakubzick, Claudia V; Kosmider, Beata; Takeda, Katsuyuki; Stranova, Thomas J; Klumm, Randal C; Delgado, Christine; Tummala, Saigiridhar; De Langhe, Stijn; Cambier, John; Haskins, Katherine; Lenz, Laurel L; Curran-Everett, Douglas

2014-11-15

Changes made in the 8th edition of the Guide for the Care and Use of Laboratory Animals included new recommendations for the amount of space for breeding female mice. Adopting the new recommendations required, in essence, the elimination of trio breeding practices for all institutions. Both public opinion and published data did not readily support the new recommendations. In response, the National Jewish Health Institutional Animal Care and Use Committee established a program to directly compare the effects of breeding format on mouse pup survival and growth. Our study showed an overall parity between trio and pairwise breeding formats on the survival and growth of the litters, suggesting that the housing recommendations for breeding female mice as stated in the current Guide for the Care and Use of Laboratory Animals should be reconsidered. Copyright © 2014 by The American Association of Immunologists, Inc.

Construction of a Thermal Vacuum Chamber for Environment Test of Triple CubeSat Mission TRIO-CINEMA

NASA Astrophysics Data System (ADS)

Jeon, Jeheon; Lee, Seongwhan; Yoon, Seyoung; Seon, Jongho; Jin, Ho; Lee, Donghun; Lin, Robert P.

2013-12-01

TRiplet Ionospheric Observatory-CubeSat for Ion, Neutron, Electron & MAgnetic fields (TRIO-CINEMA) is a CubeSat with 3.14 kg in weight and 3-U (10 × 10 × 30 cm) in size, jointly developed by Kyung Hee University and UC Berkeley to measure magnetic fields of near Earth space and detect plasma particles. When a satellite is launched into orbit, it encounters ultrahigh vacuum and extreme temperature. To verify the operation and survivability of the satellite in such an extreme space environment, experimental tests are conducted on the ground using thermal vacuum chamber. This paper describes the temperature control device and monitoring system suitable for CubeSat test environment using the thermal vacuum chamber of the School of Space Research, Kyung Hee University. To build the chamber, we use a general purpose thermal analysis program and NX 6.0 TMG program. We carry out thermal vacuum tests on the two flight models developed by Kyung Hee University based on the thermal model of the TRIO-CINEMA satellite. It is expected from this experiment that proper operation of the satellite in the space environment will be achieved.

Solo/Trio8, a membrane-associated short isoform of Trio, modulates endosome dynamics and neurite elongation.

PubMed

Sun, Ying-Jie; Nishikawa, Kaori; Yuda, Hideki; Wang, Yu-Lai; Osaka, Hitoshi; Fukazawa, Nobuna; Naito, Akira; Kudo, Yoshihisa; Wada, Keiji; Aoki, Shunsuke

2006-09-01

With DNA microarrays, we identified a gene, termed Solo, that is downregulated in the cerebellum of Purkinje cell degeneration mutant mice. Solo is a mouse homologue of rat Trio8-one of multiple Trio isoforms recently identified in rat brain. Solo/Trio8 contains N-terminal sec14-like and spectrin-like repeat domains followed by a single guanine nucleotide exchange factor 1 (GEF1) domain, but it lacks the C-terminal GEF2, immunoglobulin-like, and kinase domains that are typical of Trio. Solo/Trio8 is predominantly expressed in Purkinje neurons of the mouse brain, and expression begins following birth and increases during Purkinje neuron maturation. We identified a novel C-terminal membrane-anchoring domain in Solo/Trio8 that is required for enhanced green fluorescent protein-Solo/Trio8 localization to early endosomes (positive for both early-endosome antigen 1 [EEA1] and Rab5) in COS-7 cells and primary cultured neurons. Solo/Trio8 overexpression in COS-7 cells augmented the EEA1-positive early-endosome pool, and this effect was abolished via mutation and inactivation of the GEF domain or deletion of the C-terminal membrane-anchoring domain. Moreover, primary cultured neurons transfected with Solo/Trio8 showed increased neurite elongation that was dependent on these domains. These results suggest that Solo/Trio8 acts as an early-endosome-specific upstream activator of Rho family GTPases for neurite elongation of developing Purkinje neurons.

Managing challenging interactions with family caregivers in the cancer setting: Guidelines for clinicians (TRIO Guidelines-2).

PubMed

Laidsaar-Powell, Rebekah; Butow, Phyllis; Boyle, Frances; Juraskova, Ilona

2018-06-01

Family caregivers can, at times, add complexity to clinical encounters. Difficult family caregivers and dynamics may: derail consultation communication, reduce patient autonomy, and compromise effective clinical care. A paucity of practical strategies guiding effective clinician-family communication exists. This study aimed to develop and evaluate the first comprehensive, evidence-based guidelines (the TRIO guidelines) for oncology physicians and nurses to better manage several complex/challenging situations involving family members. TRIO Guidelines were based on a comprehensive review of literature, relevant guidelines, and feedback from an expert advisory group (n = 10). Draft guidelines underwent two rounds of evaluation via an online Delphi consensus process involving international experts (n = 35). Guidelines incorporate topic areas, strategies, and sub-strategies on managing challenging family involvement (7 topics). Example wording, behaviours and level of evidence are provided. Challenging triadic interactions require skillful navigation, and the TRIO Guidelines provide clear, specific, and evidence-based strategies for clinicians to utilise in these potentially stressful encounters. Training based on these guidelines may improve both patient care and clinician confidence. Implementation of these guidelines into medical/nursing curricula and as a component of continuing professional development programs will likely be highly beneficial. Copyright © 2018 Elsevier B.V. All rights reserved.

An Interim Report on the Educational Opportunity Centers Program: 2002-03 and 2003-04, with Select Data from 2000-02

ERIC Educational Resources Information Center

Hale, Margaret Marcus; Chan, Tsze

2006-01-01

Created in 1972, the Educational Opportunity Centers (EOC) Program, one of eight TRIO Programs, funds EOC projects at two- and four-year colleges and universities and public or private agencies or organizations to assist adults from disadvantaged backgrounds to enter or continue a postsecondary education program. Participants in EOC projects…

Evaluating TRIO Programs: A Case Study of Upward Bound Directors and Staff

ERIC Educational Resources Information Center

Carr, Yolanda Regina

2013-01-01

For over four decades, Upward Bound programs have influenced students' aspirations and goals toward attending college, college enrollment, and overall educational achievement, providing a standard for successful college preparation for historically marginalized students. Although Upward Bound has helped many Americans prepare for and earn their…

Trio combines with dock to regulate Pak activity during photoreceptor axon pathfinding in Drosophila.

PubMed

Newsome, T P; Schmidt, S; Dietzl, G; Keleman, K; Asling, B; Debant, A; Dickson, B J

2000-04-28

Correct pathfinding by Drosophila photoreceptor axons requires recruitment of p21-activated kinase (Pak) to the membrane by the SH2-SH3 adaptor Dock. Here, we identify the guanine nucleotide exchange factor (GEF) Trio as another essential component in photoreceptor axon guidance. Regulated exchange activity of one of the two Trio GEF domains is critical for accurate pathfinding. This GEF domain activates Rac, which in turn activates Pak. Mutations in trio result in projection defects similar to those observed in both Pak and dock mutants, and trio interacts genetically with Rac, Pak, and dock. These data define a signaling pathway from Trio to Rac to Pak that links guidance receptors to the growth cone cytoskeleton. We propose that distinct signals transduced via Trio and Dock act combinatorially to activate Pak in spatially restricted domains within the growth cone, thereby controlling the direction of axon extension.

34 CFR 642.24 - What are the Secretary's priorities for funding?

Code of Federal Regulations, 2011 CFR

2011-07-01

... TRIO programs. (20) The use of appropriate educational technology in the operation of projects funded... homeless children and youths, students who are foster care youth, or other disconnected students. (b) The...

34 CFR 642.24 - What are the Secretary's priorities for funding?

Code of Federal Regulations, 2012 CFR

2012-07-01

... TRIO programs. (20) The use of appropriate educational technology in the operation of projects funded... homeless children and youths, students who are foster care youth, or other disconnected students. (b) The...

34 CFR 642.24 - What are the Secretary's priorities for funding?

Code of Federal Regulations, 2014 CFR

2014-07-01

... TRIO programs. (20) The use of appropriate educational technology in the operation of projects funded... homeless children and youths, students who are foster care youth, or other disconnected students. (b) The...

34 CFR 642.24 - What are the Secretary's priorities for funding?

Code of Federal Regulations, 2013 CFR

2013-07-01

... TRIO programs. (20) The use of appropriate educational technology in the operation of projects funded... homeless children and youths, students who are foster care youth, or other disconnected students. (b) The...

The Effectiveness of the Student Support Service Program on Retention at a Rural Appalachia Community College

ERIC Educational Resources Information Center

Hodge, Deborah D.

2017-01-01

This study examined the role of TRiO Student Support Services (SSS) programs for students who persist in college. The intent of this study was to determine whether the services provided to participants in SSS helped them achieve higher grade-point averages (GPA), retention rates, and graduation rates. Student Support Services programs are designed…

The Post-Secondary Student Support Program: An Examination of Alternative Delivery Mechanisms. A Report to the Indian and Northern Affairs Canada

ERIC Educational Resources Information Center

Usher, Alex

2009-01-01

The purpose of this document is to examine the Department of Indian and Northern Affairs Canada (INAC)'s Post-Secondary Student Support Program (PSSSP) and analyse the advantages and disadvantages of both the existing system of program delivery and a trio of alternative delivery mechanisms. It does not issue any recommendation with respect to a…

Daclatasvir/asunaprevir/beclabuvir fixed-dose combination in Japanese patients with HCV genotype 1 infection.

PubMed

Toyota, Joji; Karino, Yoshiyasu; Suzuki, Fumitaka; Ikeda, Fusao; Ido, Akio; Tanaka, Katsuaki; Takaguchi, Koichi; Naganuma, Atsushi; Tomita, Eiichi; Chayama, Kazuaki; Fujiyama, Shigetoshi; Inada, Yukiko; Yoshiji, Hitoshi; Watanabe, Hideaki; Ishikawa, Hiroki; Hu, Wenhua; McPhee, Fiona; Linaberry, Misti; Yin, Philip D; Swenson, Eugene Scott; Kumada, Hiromitsu

2017-03-01

DCV-TRIO, a fixed-dose combination of daclatasvir (pangenotypic NS5A inhibitor), asunaprevir (NS3/4A protease inhibitor), and beclabuvir (non-nucleoside NS5B inhibitor), has achieved high rates of sustained virologic response at post-treatment Week 12 (SVR12) in phase 3 studies. In this phase 3 study, DCV-TRIO for 12 weeks and daclatasvir plus asunaprevir (DUAL) for 24 weeks were studied in Japanese patients infected with HCV genotype 1 (99 % genotype 1b). SVR12 rates ≥95 % were achieved in both treatment-naive (N = 152) and interferon-experienced (N = 65) cohorts treated with DCV-TRIO for 12 weeks and were comparable across patient subgroups, including patients aged ≥65 years and those with cirrhosis. DUAL recipients (N = 75) had an SVR12 rate of 87 %. In the absence of baseline resistance-associated polymorphisms at positions NS5A-Y93H or -L31, SVR12 rates were 98 % with DCV-TRIO or DUAL. Among genotype 1b-infected patients with baseline Y93H or L31 polymorphisms, 35/38 (92 %) DCV-TRIO recipients, and 7/16 (44 %) DUAL recipients achieved SVR12. Adverse events, mostly liver related, led to treatment discontinuation in 10 % of DCV-TRIO recipients. In this group, SVR12 was achieved by 3/9 patients who discontinued before Week 4 and by 12/12 patients who completed ≥4 weeks of DCV-TRIO. Treatment-related serious adverse events occurred in 4 and 3 % of DCV-TRIO and DUAL recipients, respectively. Seven patients (9 %) discontinued DUAL due to adverse events. No deaths occurred. SVR12 was achieved by 96 % of Japanese patients with HCV genotype 1 infection after 12 weeks of treatment with the DCV-TRIO regimen. DCV-TRIO and DUAL exhibited comparable safety profiles.

Supportive Measures: An Analysis of the Trio Program--Student Support Services at East Tennessee State University from 2001-2004

ERIC Educational Resources Information Center

Strode, Christopher N.

2013-01-01

The purpose of this study was to examine the academic performance of the first-time, full-time, traditional-aged students in the Student Support Services program at East Tennessee State University. This was accomplished by comparing their academic performance with the academic performance of first-time, full-time, traditional-aged non-SSS…

The Persistence and Retention of Students Participating in a Student Success Program

ERIC Educational Resources Information Center

Kitchens, Karen Westerman

2016-01-01

The purpose of this mixed methods study was to examine and explore the institutional policy levers that facilitate successful educational outcomes in a TRiO funded Student Success Program at a large, urban research university in the Mid-South. Three research questions guided the study: (1) how do the educational outcomes of the university's TRiO…

An integrated software suite for surface-based analyses of cerebral cortex.

PubMed

Van Essen, D C; Drury, H A; Dickson, J; Harwell, J; Hanlon, D; Anderson, C H

2001-01-01

The authors describe and illustrate an integrated trio of software programs for carrying out surface-based analyses of cerebral cortex. The first component of this trio, SureFit (Surface Reconstruction by Filtering and Intensity Transformations), is used primarily for cortical segmentation, volume visualization, surface generation, and the mapping of functional neuroimaging data onto surfaces. The second component, Caret (Computerized Anatomical Reconstruction and Editing Tool Kit), provides a wide range of surface visualization and analysis options as well as capabilities for surface flattening, surface-based deformation, and other surface manipulations. The third component, SuMS (Surface Management System), is a database and associated user interface for surface-related data. It provides for efficient insertion, searching, and extraction of surface and volume data from the database.

An integrated software suite for surface-based analyses of cerebral cortex

NASA Technical Reports Server (NTRS)

Van Essen, D. C.; Drury, H. A.; Dickson, J.; Harwell, J.; Hanlon, D.; Anderson, C. H.

2001-01-01

The authors describe and illustrate an integrated trio of software programs for carrying out surface-based analyses of cerebral cortex. The first component of this trio, SureFit (Surface Reconstruction by Filtering and Intensity Transformations), is used primarily for cortical segmentation, volume visualization, surface generation, and the mapping of functional neuroimaging data onto surfaces. The second component, Caret (Computerized Anatomical Reconstruction and Editing Tool Kit), provides a wide range of surface visualization and analysis options as well as capabilities for surface flattening, surface-based deformation, and other surface manipulations. The third component, SuMS (Surface Management System), is a database and associated user interface for surface-related data. It provides for efficient insertion, searching, and extraction of surface and volume data from the database.

Trilinos I/O Support (Trios)

DOE PAGES

Oldfield, Ron A.; Sjaardema, Gregory D.; Lofstead II, Gerald F.; ...

2012-01-01

Trilinos I/O Support (Trios) is a new capability area in Trilinos that serves two important roles: (1) it provides and supports I/O libraries used by in-production scientific codes; (2) it provides a research vehicle for the evaluation and distribution of new techniques to improve I/O on advanced platforms. This paper provides a brief overview of the production-grade I/O libraries in Trios as well as some of the ongoing research efforts that contribute to the experimental libraries in Trios.

An Integrated Software Suite for Surface-based Analyses of Cerebral Cortex

PubMed Central

Van Essen, David C.; Drury, Heather A.; Dickson, James; Harwell, John; Hanlon, Donna; Anderson, Charles H.

2001-01-01

The authors describe and illustrate an integrated trio of software programs for carrying out surface-based analyses of cerebral cortex. The first component of this trio, SureFit (Surface Reconstruction by Filtering and Intensity Transformations), is used primarily for cortical segmentation, volume visualization, surface generation, and the mapping of functional neuroimaging data onto surfaces. The second component, Caret (Computerized Anatomical Reconstruction and Editing Tool Kit), provides a wide range of surface visualization and analysis options as well as capabilities for surface flattening, surface-based deformation, and other surface manipulations. The third component, SuMS (Surface Management System), is a database and associated user interface for surface-related data. It provides for efficient insertion, searching, and extraction of surface and volume data from the database. PMID:11522765

76 FR 338 - Notice of Proposed Information Collection Requests

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-04

... feasibility of a promising practices study of Upward Bound that uses a rigorous quasi-experimental design. The design and feasibility report will develop a set of design options for conducting a study of Upward Bound and examine their applicability to studies of other TRIO programs. If a rigorous evaluation design...

Ovulation rate, antral follicle count, and circulating anti-Müllerian hormone in Trio allele carriers, a novel high fecundity bovine genotype.

PubMed

García-Guerra, Alvaro; Motta, Jéssica C L; Melo, Leonardo F; Kirkpatrick, Brian W; Wiltbank, Milo C

2017-10-01

High fecundity genotypes in sheep are a valuable model to study the physiological mechanisms underlying follicle selection and the control of ovulation rate. Similar genotypes in cattle had not been described until the recent identification of a major bovine allele, termed Trio, which had a large effect on ovulation rate. The present study was designed to evaluate ovulation rate, antral follicle count (AFC), circulating ant-müllerian hormone (AMH), and the association among these measures in unstimulated and superstimulated Trio carrier cattle. We hypothesized that AFC and AMH would be variable among individual cows but would be similar between Trio carriers and non-carrier control cows and that there would be no association between these measures of follicle numbers and ovulation rate. In experiment 1, ovulation rate was determined during 4 consecutive estrous cycles in Trio carriers (n = 34) and non-carrier controls (n = 27). Ovulation rate, on average, was greater (P < 0.01) in Trio carriers (3.5 ± 0.2) compared to non-carrier controls (1.1 ± 0.1) with ∼70% of carrier cycles (n = 136) having 3-4 ovulations while only ∼5% had single ovulations. In contrast, non-carrier cycles (n = 108) were mostly single ovulation (89%) with none having more than two ovulations. In experiment 2, AFC, determined at wave emergence, was not different (P = 0.54) between Trio carriers (24.5 ± 1.3; n = 45) and non-carrier controls (23.1 ± 0.9; n = 37), and no correlation was found between AFC and mean ovulation rate in either genotype (r = -0.009 and r = -0.07; P > 0.70, respectively). In Experiment 3, circulating AMH was also not different between genotypes (P = 0.65) while correlations were found between AFC and AMH in Trio carriers (r = 0.43; P = 0.05; n = 27) and non-carrier controls (r = 0.78; P < 0.01; n = 19). In experiment 4, AFC and AMH were determined in Trio-carriers (n = 9) in relation to a synchronized follicular wave which was unstimulated or stimulated with exogenous FSH. Stimulation with FSH increased ovulation rate, compared to unstimulated Trio carriers, however no association was found between AFC or AMH and ovulation rate regardless of whether superstimulation with exogenous FSH was used. In conclusion, the novel high fecundity bovine genotype Trio, results in consistent multiple ovulations despite having similar AFC and AMH. Therefore, our results suggest that differences in antral follicle numbers during the final stages of follicle development are not a key component of the mechanism underlying multiple ovulations in Trio carriers. Copyright © 2017. Published by Elsevier Inc.

jsc2014e049399

NASA Image and Video Library

2014-05-16

15-43-34-2: At the Baikonur Cosmodrome in Kazakhstan, Expedition 40/41 Soyuz Commander Max Suraev of the Russian Federal Space Agency (Roscosmos, left), Flight Engineer Reid Wiseman of NASA (center) and Flight Engineer Alexander Gerst of the European Space Agency (right) display their Russian Sokol launch and entry suits on their shoulders May 16 during a dress rehearsal “fit check”. The trio will launch from Baikonur on May 29, Kazakh time, on the Soyuz TMA-13M spacecraft for a 5 ½ month mission on the International Space Station. NASA/Victor Zelentsov

Self-Regulated Learning and Ethnic/Racial Variables: Predicting Minority First-Generation College Students' Persistence

ERIC Educational Resources Information Center

Moore, John S., III.

2013-01-01

The purpose of this study was to investigate how self-regulated learning and ethnic/racial variables predict minority first-generation college student persistence and related constructs. Participants were drawn nationally from the U.S. Department of Education funded TRiO Student Support Services Programs. Additional participants from the Talent…

Direct qPCR quantification using the Quantifiler(®) Trio DNA quantification kit.

PubMed

Liu, Jason Yingjie

2014-11-01

The effectiveness of a direct quantification assay is essential to the adoption of the combined direct quantification/direct STR workflow. In this paper, the feasibility of using the Quantifiler(®) Trio DNA quantification kit for the direct quantification of forensic casework samples was investigated. Both low-level touch DNA samples and blood samples were collected on PE swabs and quantified directly. The increased sensitivity of the Quantifiler(®) Trio kit enabled the detection of less than 10pg of DNA in unprocessed touch samples and also minimizes the stochastic effect experienced by different targets in the same sample. The DNA quantity information obtained from a direct quantification assay using the Quantifiler(®) Trio kit can also be used to accurately estimate the optimal input DNA quantity for a direct STR amplification reaction. The correlation between the direct quantification results (Quantifiler(®) Trio kit) and the direct STR results (GlobalFiler™ PCR amplification kit(*)) for low-level touch DNA samples indicates that direct quantification using the Quantifiler(®) Trio DNA quantification kit is more reliable than the Quantifiler(®) Duo DNA quantification kit for predicting the STR results of unprocessed touch DNA samples containing less than 10pg of DNA. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Neurodevelopmental disease-associated de novo mutations and rare sequence variants affect TRIO GDP/GTP exchange factor activity.

PubMed

Katrancha, Sara M; Wu, Yi; Zhu, Minsheng; Eipper, Betty A; Koleske, Anthony J; Mains, Richard E

2017-12-01

Bipolar disorder, schizophrenia, autism and intellectual disability are complex neurodevelopmental disorders, debilitating millions of people. Therapeutic progress is limited by poor understanding of underlying molecular pathways. Using a targeted search, we identified an enrichment of de novo mutations in the gene encoding the 330-kDa triple functional domain (TRIO) protein associated with neurodevelopmental disorders. By generating multiple TRIO antibodies, we show that the smaller TRIO9 isoform is the major brain protein product, and its levels decrease after birth. TRIO9 contains two guanine nucleotide exchange factor (GEF) domains with distinct specificities: GEF1 activates both Rac1 and RhoG; GEF2 activates RhoA. To understand the impact of disease-associated de novo mutations and other rare sequence variants on TRIO function, we utilized two FRET-based biosensors: a Rac1 biosensor to study mutations in TRIO (T)GEF1, and a RhoA biosensor to study mutations in TGEF2. We discovered that one autism-associated de novo mutation in TGEF1 (K1431M), at the TGEF1/Rac1 interface, markedly decreased its overall activity toward Rac1. A schizophrenia-associated rare sequence variant in TGEF1 (F1538Intron) was substantially less active, normalized to protein level and expressed poorly. Overall, mutations in TGEF1 decreased GEF1 activity toward Rac1. One bipolar disorder-associated rare variant (M2145T) in TGEF2 impaired inhibition by the TGEF2 pleckstrin-homology domain, resulting in dramatically increased TGEF2 activity. Overall, genetic damage to both TGEF domains altered TRIO catalytic activity, decreasing TGEF1 activity and increasing TGEF2 activity. Importantly, both GEF changes are expected to decrease neurite outgrowth, perhaps consistent with their association with neurodevelopmental disorders. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

A Comparison of Phasing Algorithms for Trios and Unrelated Individuals

PubMed Central

Marchini, Jonathan; Cutler, David; Patterson, Nick; Stephens, Matthew; Eskin, Eleazar; Halperin, Eran; Lin, Shin; Qin, Zhaohui S.; Munro, Heather M.; Abecasis, Gonçalo R.; Donnelly, Peter

2006-01-01

Knowledge of haplotype phase is valuable for many analysis methods in the study of disease, population, and evolutionary genetics. Considerable research effort has been devoted to the development of statistical and computational methods that infer haplotype phase from genotype data. Although a substantial number of such methods have been developed, they have focused principally on inference from unrelated individuals, and comparisons between methods have been rather limited. Here, we describe the extension of five leading algorithms for phase inference for handling father-mother-child trios. We performed a comprehensive assessment of the methods applied to both trios and to unrelated individuals, with a focus on genomic-scale problems, using both simulated data and data from the HapMap project. The most accurate algorithm was PHASE (v2.1). For this method, the percentages of genotypes whose phase was incorrectly inferred were 0.12%, 0.05%, and 0.16% for trios from simulated data, HapMap Centre d'Etude du Polymorphisme Humain (CEPH) trios, and HapMap Yoruban trios, respectively, and 5.2% and 5.9% for unrelated individuals in simulated data and the HapMap CEPH data, respectively. The other methods considered in this work had comparable but slightly worse error rates. The error rates for trios are similar to the levels of genotyping error and missing data expected. We thus conclude that all the methods considered will provide highly accurate estimates of haplotypes when applied to trio data sets. Running times differ substantially between methods. Although it is one of the slowest methods, PHASE (v2.1) was used to infer haplotypes for the 1 million–SNP HapMap data set. Finally, we evaluated methods of estimating the value of r2 between a pair of SNPs and concluded that all methods estimated r2 well when the estimated value was ⩾0.8. PMID:16465620

The system design of TRIO cinema Mission

NASA Astrophysics Data System (ADS)

Jin, Ho; Seon, Jongho; Kim, Khan-Hyuk; Lee, Dong-Hun; Kim, Kap-Sung; Lin, Robert; Parks, George; Tindall, Craig; Horbury, T. S.; Larson, Davin; Sample, John

TRIO (Triplet Ionospheric Observatory) CINEMA ( Cubesat for Ion, Neutral, Electron, MAg-netic fields) is a space science mission with three identical cubesats. The main scientific objec-tives are a multi-observation of ionospheric ENA (Energetic Neutral Atom) imaging, ionospheric signature of suprathermal electrons and ions and complementary measurements of magnetic fields for particle data. For this, Main payloads consist of a suprathermal electron, ion, neutral (STEIN) instrument and a 3-axis magnetometer of magnetoresistive sensors. The CINEMA is a 3-unit CubeSat, which translates to a 10 cm x 10 cm x 30 cm in volume and no more than four kilograms in mass. An attitude control system (ACS) uses torque coils, a sun sensor and the magnetometers and spin CINEMA spcaecraft 4 rpm with the spin axis perpendicular to the ecliptic plane. CINEMA will be placed into a high inclination low earth orbit that crosses the auroral zone and cusp. Three institutes are collaborating to develop CINEMA cubesats: i) two cubesats by Kyung Hee University (KHU) under their World Class University (WCU) program, ii) one cubesat by UC Berkeley under the NSF support, and iii) three magnetometers are provide by Imperial College, respectively. In this paper, we describe the system design and their performance of TR IO cinema mission. TRIO cinema's development of miniature in-strument and spacecraft spinning operation will play an important role for future nanosatellite space missions

The transcription factors SOX9 and SOX5/SOX6 cooperate genome-wide through super-enhancers to drive chondrogenesis

PubMed Central

Liu, Chia-Feng; Lefebvre, Véronique

2015-01-01

SOX9 is a transcriptional activator required for chondrogenesis, and SOX5 and SOX6 are closely related DNA-binding proteins that critically enhance its function. We use here genome-wide approaches to gain novel insights into the full spectrum of the target genes and modes of action of this chondrogenic trio. Using the RCS cell line as a faithful model for proliferating/early prehypertrophic growth plate chondrocytes, we uncover that SOX6 and SOX9 bind thousands of genomic sites, frequently and most efficiently near each other. SOX9 recognizes pairs of inverted SOX motifs, whereas SOX6 favors pairs of tandem SOX motifs. The SOX proteins primarily target enhancers. While binding to a small fraction of typical enhancers, they bind multiple sites on almost all super-enhancers (SEs) present in RCS cells. These SEs are predominantly linked to cartilage-specific genes. The SOX proteins effectively work together to activate these SEs and are required for in vivo expression of their associated genes. These genes encode key regulatory factors, including the SOX trio proteins, and all essential cartilage extracellular matrix components. Chst11, Fgfr3, Runx2 and Runx3 are among many other newly identified SOX trio targets. SOX9 and SOX5/SOX6 thus cooperate genome-wide, primarily through SEs, to implement the growth plate chondrocyte differentiation program. PMID:26150426

Accuracy of digital impressions of multiple dental implants: an in vitro study.

PubMed

Vandeweghe, Stefan; Vervack, Valentin; Dierens, Melissa; De Bruyn, Hugo

2017-06-01

Studies demonstrated that the accuracy of intra-oral scanners can be compared with conventional impressions for most indications. However, little is known about their applicability to take impressions of multiple implants. The aim of this study was to evaluate the accuracy of four intra-oral scanners when applied for implant impressions in the edentulous jaw. An acrylic mandibular cast containing six external connection implants (region 36, 34, 32, 42, 44 and 46) with PEEK scanbodies was scanned using four intra-oral scanners: the Lava C.O.S. and the 3M True Definition, Cerec Omnicam and 3Shape Trios. Each model was scanned 10 times with every intra-oral scanner. As a reference, a highly accurate laboratory scanner (104i, Imetric, Courgenay, Switzerland) was used. The scans were imported into metrology software (Geomagic Qualify 12) for analyses. Accuracy was measured in terms of trueness (comparing test and reference) and precision (determining the deviation between different test scans). Mann-Whitney U-test and Wilcoxon signed rank test were used to detect statistically significant differences in trueness and precision respectively. The mean trueness was 0.112 mm for Lava COS, 0.035 mm for 3M TrueDef, 0.028 mm for Trios and 0.061 mm for Cerec Omnicam. There was no statistically significant difference between 3M TrueDef and Trios (P = 0.262). Cerec Omnicam was less accurate than 3M TrueDef (P = 0.013) and Trios (P = 0.005), but more accurate compared to Lava COS (P = 0.007). Lava COS was also less accurate compared to 3M TrueDef (P = 0.005) and Trios (P = 0.005). The mean precision was 0.066 mm for Lava COS, 0.030 mm for 3M TrueDef, 0.033 mm for Trios and 0.059 mm for Cerec Omnicam. There was no statistically significant difference between 3M TrueDef and Trios (P = 0.119). Cerec Omnicam was less accurate compared to 3M TrueDef (P < 0.001) and Trios (P < 0.001), but no difference was found with Lava COS (P = 0.169). Lava COS was also less accurate compared to 3M TrueDef (P < 0.001) and Trios (P < 0.001). Based on the findings of this in vitro study, the 3M True Definition and Trios scanner demonstrated the highest accuracy. The Lava COS was found not suitable for taking implant impressions for a cross-arch bridge in the edentulous jaw. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Expedition 43 Preflight

NASA Image and Video Library

2015-03-06

Expedition 43 NASA Astronaut Scott Kelly, left, Russian cosmonaut Gennady Padalka of the Russian Federal Space Agency (Roscosmos), center, and Russian cosmonaut Mikhail Kornienko of Roscosmos pose for a photograph in front of St. Basil's Cathedral in Moscow as part of traditional pre-launch ceremonies, Friday, March 6, 2015. The trio is preparing for launch to the International Space Station in their Soyuz TMA-16M spacecraft from the Baikonur Cosmodrome in Kazakhstan March 28, Kazakh time. As the one-year crew, Kelly and Kornienko will return to Earth on Soyuz TMA-18M in March 2016. Photo Credit: (NASA/Bill Ingalls)

Zero Robotics at Kennedy Space Center Visitor Complex

NASA Image and Video Library

2017-08-11

A trio of programmable off-the-shelf Sphero robots are shown at the Center for Space Education at NASA's Kennedy Space Center in Florida. The Spheros were available for students to practice their programming skills during "loss of signal" times when the connection to the International Space Station was temporarily unavailable. Teams from across the state of Florida were gathered at Kennedy for the finals of the Zero Robotics Middle School Summer Program national championship. The five-week program allows rising sixth- through ninth-graders to write programs for small satellites called SPHERES (Synchronized, Position, Hold, Engage, Reorient, Experimental Satellites). Finalists saw their code tested aboard the orbiting laboratory.

Universal cell frame for high-pressure water electrolyzer and electrolyzer including the same

DOEpatents

Schmitt, Edwin W.; Norman, Timothy J.

2013-01-08

Universal cell frame generic for use as an anode frame and as a cathode frame in a water electrolyzer. According to one embodiment, the universal cell frame includes a unitary annular member having a central opening. Four trios of transverse openings are provided in the annular member, each trio being spaced apart by about 90 degrees. A plurality of internal radial passageways fluidly interconnect the central opening and each of the transverse openings of two diametrically-opposed trios of openings, the other two trios of openings lacking corresponding radial passageways. Sealing ribs are provided on the top and bottom surfaces of the annular member. The present invention is also directed at a water electrolyzer that includes two such cell frames, one being used as the anode frame and the other being used as the cathode frame, the cathode frame being rotated 90 degrees relative to the anode frame.

Investigation of previously implicated genetic variants in chronic tic disorders: a transmission disequilibrium test approach.

PubMed

Abdulkadir, Mohamed; Londono, Douglas; Gordon, Derek; Fernandez, Thomas V; Brown, Lawrence W; Cheon, Keun-Ah; Coffey, Barbara J; Elzerman, Lonneke; Fremer, Carolin; Fründt, Odette; Garcia-Delgar, Blanca; Gilbert, Donald L; Grice, Dorothy E; Hedderly, Tammy; Heyman, Isobel; Hong, Hyun Ju; Huyser, Chaim; Ibanez-Gomez, Laura; Jakubovski, Ewgeni; Kim, Young Key; Kim, Young Shin; Koh, Yun-Joo; Kook, Sodahm; Kuperman, Samuel; Leventhal, Bennett; Ludolph, Andrea G; Madruga-Garrido, Marcos; Maras, Athanasios; Mir, Pablo; Morer, Astrid; Müller-Vahl, Kirsten; Münchau, Alexander; Murphy, Tara L; Plessen, Kerstin J; Roessner, Veit; Shin, Eun-Young; Song, Dong-Ho; Song, Jungeun; Tübing, Jennifer; van den Ban, Els; Visscher, Frank; Wanderer, Sina; Woods, Martin; Zinner, Samuel H; King, Robert A; Tischfield, Jay A; Heiman, Gary A; Hoekstra, Pieter J; Dietrich, Andrea

2018-04-01

Genetic studies in Tourette syndrome (TS) are characterized by scattered and poorly replicated findings. We aimed to replicate findings from candidate gene and genome-wide association studies (GWAS). Our cohort included 465 probands with chronic tic disorder (93% TS) and both parents from 412 families (some probands were siblings). We assessed 75 single nucleotide polymorphisms (SNPs) in 465 parent-child trios; 117 additional SNPs in 211 trios; and 4 additional SNPs in 254 trios. We performed SNP and gene-based transmission disequilibrium tests and compared nominally significant SNP results with those from a large independent case-control cohort. After quality control 71 SNPs were available in 371 trios; 112 SNPs in 179 trios; and 3 SNPs in 192 trios. 17 were candidate SNPs implicated in TS and 2 were implicated in obsessive-compulsive disorder (OCD) or autism spectrum disorder (ASD); 142 were tagging SNPs from eight monoamine neurotransmitter-related genes (including dopamine and serotonin); 10 were top SNPs from TS GWAS; and 13 top SNPs from attention-deficit/hyperactivity disorder, OCD, or ASD GWAS. None of the SNPs or genes reached significance after adjustment for multiple testing. We observed nominal significance for the candidate SNPs rs3744161 (TBCD) and rs4565946 (TPH2) and for five tagging SNPs; none of these showed significance in the independent cohort. Also, SLC1A1 in our gene-based analysis and two TS GWAS SNPs showed nominal significance, rs11603305 (intergenic) and rs621942 (PICALM). We found no convincing support for previously implicated genetic polymorphisms. Targeted re-sequencing should fully appreciate the relevance of candidate genes.

A Trio of Craters: Munch, Sander, and Poe

NASA Image and Video Library

2009-02-17

Sander, Munch, and Poe are a trio of impact craters within the Caloris impact basin. Munch and Poe were recently named, while Sander received its name in the first set of feature names after MESSENGER’s first Mercury flyby.

Expedition 43 Preflight

NASA Image and Video Library

2015-03-06

Expedition 43 NASA Astronaut Scott Kelly, left, Russian cosmonaut Gennady Padalka of the Russian Federal Space Agency (Roscosmos), center, and Russian cosmonaut Mikhail Kornienko of Roscosmos walk along the Kremlin Wall in Red Square to leave roses at the site where Russian space icons are interred as part of traditional pre-launch ceremonies, Friday, March 6, 2015, Moscow, Russia. The trio is preparing for launch to the International Space Station in their Soyuz TMA-16M spacecraft from the Baikonur Cosmodrome in Kazakhstan March 28, Kazakh time. As the one-year crew, Kelly and Kornienko will return to Earth on Soyuz TMA-18M in March 2016. Photo Credit: (NASA/Bill Ingalls)

Evaluation of the association of polymorphisms in EYA1, environmental factors, and non-syndromic orofacial clefts in Western Han Chinese.

PubMed

Zeng, Ni; Wu, Jun; Zhu, Wen-Chao; Shi, Bing; Jia, Zhong-Lin

2015-11-01

Non-syndromic orofacial clefts (NSOCs) are complex disease involving genetic triggers, environmental factors, and their interplay. Recent studies demonstrated that EYA1, a member of eye absent gene family, might contribute to NSOCs. We investigated three single nucleotide polymorphisms (SNPs) and eight environmental factors (multivitamin, folic acid and calcium supplementation history, maternal alcohol consumption, common cold history, maternal smoking and environmental tobacco smoke in the first trimester, and paternal smoking in the 3 months before pregnancy) among 294 case-parent trios and 183 individual controls in western Han Chinese to evaluate the relationship between EYA1, environmental factors, and NSOCs. To be better known the gene's role in the etiology of NSOCs, we performed statistical analysis in different aspects including the linkage disequilibrium test, transmission disequilibrium test, haplotype analysis, multiple logistic regression analysis, and conditional logistic regression analysis. Allele C at rs3779748 showed an over-transmission in NSCL/P trios (P = 0.03), and genotype A/A at rs10094908 was under-transmitted among NSCL/P trios (P = 0.03), whereas over-transmitted among NSCPO trios (P = 0.02). The haplotype GC of rs10094908-rs3779748 was over-transmitted among NSCL/P trios (P = 0.05) and NSCPO trios (P = 0.05), respectively. Maternal common cold history, environmental tobacco smoke, and maternal alcohol consumption during the first trimester of pregnancy were risk factors for NSOCs, while calcium supplementation during the first trimester showed a protective effect. No evidence of interactions between EYA1 and environmental factors was found. These results revealed an association between EYA1, some environmental factors, and NSOCs in western Han Chinese. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

A novel multiplex real-time PCR assay for the concurrent detection of hepatitis A, B and C viruses in patients with acute hepatitis.

PubMed

Park, Yongjung; Kim, Beom Seok; Choi, Kyu Hun; Shin, Dong Ho; Lee, Mi Jung; Cho, Yonggeun; Kim, Hyon-Suk

2012-01-01

A novel multiplex real-time PCR assay for concurrent detection of hepatitis viruses was evaluated for its clinical performance in screening patients with acute hepatitis. A total of 648 serum samples were collected from patients with acute symptoms of hepatitis. Concurrent detection of nucleic acids of HAV, HBV and HCV was performed using the Magicplex™ HepaTrio Real-time Detection test. Serum nucleic acid levels of HBV and HCV were also quantified by the Cobas® AmpliPrep/Cobas® TaqMan® (CAP/CTM) HBV and HCV tests. Patients' medical records were also reviewed. Concordance rates between the results from the HepaTrio and the CAP/CTM tests for the detection of HBV and HCV were 94.9% (k = 0.88) and 99.2% (k = 0.98), respectively. The cycle threshold values with the HepaTrio test were also correlated well with the levels of HBV DNA (r = -0.9230) and HCV RNA (r = -0.8458). The sensitivity and specificity of the HepaTrio test were 93.8% and 98.2%, respectively, for detecting HBV infection, and 99.1% and 100.0%, respectively, for HCV infection. For the HepaTrio test, 21 (3.2%) cases were positive for both HBV and HCV. Among the positive cases, 6 (0.9%) were true coinfections. This test also detected 18 (2.8%) HAV positives. The HepaTrio test demonstrated good clinical performance and produced results that agreed well with those of the CAP/CTM assays, especially for the detection of HCV. This assay was also able to detect HAV RNA from anti-HAV IgM-positive individuals. Therefore, this new multiplex PCR assay could be useful for the concurrent detection of the three hepatitis viruses.

Genome-wide Association Study of Obsessive-Compulsive Disorder

PubMed Central

Stewart, S Evelyn; Yu, Dongmei; Scharf, Jeremiah M; Neale, Benjamin M; Fagerness, Jesen A; Mathews, Carol A; Arnold, Paul D; Evans, Patrick D; Gamazon, Eric R; Osiecki, Lisa; McGrath, Lauren; Haddad, Stephen; Crane, Jacquelyn; Hezel, Dianne; Illman, Cornelia; Mayerfeld, Catherine; Konkashbaev, Anuar; Liu, Chunyu; Pluzhnikov, Anna; Tikhomirov, Anna; Edlund, Christopher K; Rauch, Scott L; Moessner, Rainald; Falkai, Peter; Maier, Wolfgang; Ruhrmann, Stephan; Grabe, Hans-Jörgen; Lennertz, Leonard; Wagner, Michael; Bellodi, Laura; Cavallini, Maria Cristina; Richter, Margaret A; Cook, Edwin H; Kennedy, James L; Rosenberg, David; Stein, Dan J; Hemmings, Sian MJ; Lochner, Christine; Azzam, Amin; Chavira, Denise A; Fournier, Eduardo; Garrido, Helena; Sheppard, Brooke; Umaña, Paul; Murphy, Dennis L; Wendland, Jens R; Veenstra-VanderWeele, Jeremy; Denys, Damiaan; Blom, Rianne; Deforce, Dieter; Van Nieuwerburgh, Filip; Westenberg, Herman GM; Walitza, Susanne; Egberts, Karin; Renner, Tobias; Miguel, Euripedes Constantino; Cappi, Carolina; Hounie, Ana G; Conceição do Rosário, Maria; Sampaio, Aline S; Vallada, Homero; Nicolini, Humberto; Lanzagorta, Nuria; Camarena, Beatriz; Delorme, Richard; Leboyer, Marion; Pato, Carlos N; Pato, Michele T; Voyiaziakis, Emanuel; Heutink, Peter; Cath, Danielle C; Posthuma, Danielle; Smit, Jan H; Samuels, Jack; Bienvenu, O Joseph; Cullen, Bernadette; Fyer, Abby J; Grados, Marco A; Greenberg, Benjamin D; McCracken, James T; Riddle, Mark A; Wang, Ying; Coric, Vladimir; Leckman, James F; Bloch, Michael; Pittenger, Christopher; Eapen, Valsamma; Black, Donald W; Ophoff, Roel A; Strengman, Eric; Cusi, Daniele; Turiel, Maurizio; Frau, Francesca; Macciardi, Fabio; Gibbs, J Raphael; Cookson, Mark R; Singleton, Andrew; Hardy, John; Crenshaw, Andrew T; Parkin, Melissa A; Mirel, Daniel B; Conti, David V; Purcell, Shaun; Nestadt, Gerald; Hanna, Gregory L; Jenike, Michael A; Knowles, James A; Cox, Nancy; Pauls, David L

2014-01-01

Obsessive-compulsive disorder (OCD) is a common, debilitating neuropsychiatric illness with complex genetic etiology. The International OCD Foundation Genetics Collaborative (IOCDF-GC) is a multi-national collaboration established to discover the genetic variation predisposing to OCD. A set of individuals affected with DSM-IV OCD, a subset of their parents, and unselected controls, were genotyped with several different Illumina SNP microarrays. After extensive data cleaning, 1,465 cases, 5,557 ancestry-matched controls and 400 complete trios remained, with a common set of 469,410 autosomal and 9,657 X-chromosome SNPs. Ancestry-stratified case-control association analyses were conducted for three genetically-defined subpopulations and combined in two meta-analyses, with and without the trio-based analysis. In the case-control analysis, the lowest two p-values were located within DLGAP1 (p=2.49×10-6 and p=3.44×10-6), a member of the neuronal postsynaptic density complex. In the trio analysis, rs6131295, near BTBD3, exceeded the genome-wide significance threshold with a p-value=3.84 × 10-8. However, when trios were meta-analyzed with the combined case-control samples, the p-value for this variant was 3.62×10-5, losing genome-wide significance. Although no SNPs were identified to be associated with OCD at a genome-wide significant level in the combined trio-case-control sample, a significant enrichment of methylation-QTLs (p<0.001) and frontal lobe eQTLs (p=0.001) was observed within the top-ranked SNPs (p<0.01) from the trio-case-control analysis, suggesting these top signals may have a broad role in gene expression in the brain, and possibly in the etiology of OCD. PMID:22889921

Idiographic duo-trio tests using a constant-reference based on preference of each consumer: Sample presentation sequence in difference test can be customized for individual consumers to reduce error.

PubMed

Kim, Min-A; Sim, Hye-Min; Lee, Hye-Seong

2016-11-01

As reformulations and processing changes are increasingly needed in the food industry to produce healthier, more sustainable, and cost effective products while maintaining superior quality, reliable measurements of consumers' sensory perception and discrimination are becoming more critical. Consumer discrimination methods using a preferred-reference duo-trio test design have been shown to be effective in improving the discrimination performance by customizing sample presentation sequences. However, this design can add complexity to the discrimination task for some consumers, resulting in more errors in sensory discrimination. The objective of the present study was to investigate the effects of different types of test instructions using the preference-reference duo-trio test design where a paired-preference test is followed by 6 repeated preferred-reference duo-trio tests, in comparison to the analytical method using the balanced-reference duo-trio. Analyses of d' estimates (product-related measure) and probabilistic sensory discriminators in momentary numbers of subjects showing statistical significance (subject-related measure) revealed that only preferred-reference duo-trio test using affective reference-framing, either by providing no information about the reference or information on a previously preferred sample, improved the sensory discrimination more than the analytical method. No decrease in discrimination performance was observed with any type of instruction, confirming that consumers could handle the test methods. These results suggest that when repeated tests are feasible, using the affective discrimination method would be operationally more efficient as well as ecologically more reliable for measuring consumers' sensory discrimination ability. Copyright © 2016 Elsevier Ltd. All rights reserved.

A Phenomenological Study of What Black Male TRiO Student Support Services Program Members Attribute to Their Decision to Drop out of College

ERIC Educational Resources Information Center

Jones, LaTonya S.

2011-01-01

Compared to other races, the college achievement gap is largest between Black men and women where females earn twice as many degrees as their male counterparts (National Center for Education Statistics, 2010, Status and trends in the education of racial and ethnic minorities). Many Black men attempt college and eventually drop out forfeiting their…

Cross-Species Analyses Identify the BNIP-2 and Cdc42GAP Homology (BCH) Domain as a Distinct Functional Subclass of the CRAL_TRIO/Sec14 Superfamily

PubMed Central

Gupta, Anjali Bansal; Wee, Liang En; Zhou, Yi Ting; Hortsch, Michael; Low, Boon Chuan

2012-01-01

The CRAL_TRIO protein domain, which is unique to the Sec14 protein superfamily, binds to a diverse set of small lipophilic ligands. Similar domains are found in a range of different proteins including neurofibromatosis type-1, a Ras GTPase-activating Protein (RasGAP) and Rho guanine nucleotide exchange factors (RhoGEFs). Proteins containing this structural protein domain exhibit a low sequence similarity and ligand specificity while maintaining an overall characteristic three-dimensional structure. We have previously demonstrated that the BNIP-2 and Cdc42GAP Homology (BCH) protein domain, which shares a low sequence homology with the CRAL_TRIO domain, can serve as a regulatory scaffold that binds to Rho, RhoGEFs and RhoGAPs to control various cell signalling processes. In this work, we investigate 175 BCH domain-containing proteins from a wide range of different organisms. A phylogenetic analysis with ∼100 CRAL_TRIO and similar domains from eight representative species indicates a clear distinction of BCH-containing proteins as a novel subclass within the CRAL_TRIO/Sec14 superfamily. BCH-containing proteins contain a hallmark sequence motif R(R/K)h(R/K)(R/K)NL(R/K)xhhhhHPs (‘h’ is large and hydrophobic residue and ‘s’ is small and weekly polar residue) and can be further subdivided into three unique subtypes associated with BNIP-2-N, macro- and RhoGAP-type protein domains. A previously unknown group of genes encoding ‘BCH-only’ domains is also identified in plants and arthropod species. Based on an analysis of their gene-structure and their protein domain context we hypothesize that BCH domain-containing genes evolved through gene duplication, intron insertions and domain swapping events. Furthermore, we explore the point of divergence between BCH and CRAL-TRIO proteins in relation to their ability to bind small GTPases, GAPs and GEFs and lipid ligands. Our study suggests a need for a more extensive analysis of previously uncharacterized BCH, ‘BCH-like’ and CRAL_TRIO-containing proteins and their significance in regulating signaling events involving small GTPases. PMID:22479462

Culture Clash Invades Miami: Oral Histories and Ethnography Center Stage

ERIC Educational Resources Information Center

Garcia, David G.

2008-01-01

Using a critical race theory (CRT) framework, this article compares the playwriting methods of the Chicano--Latino theater trio, Culture Clash, to a counterstorytelling methodology. The author uncovers the tenets of a critical race theater in the trio's site-specific ethnographic play, "Radio Mambo: Culture Clash Invades Miami". He…

The TAKEOVER TRIO

ERIC Educational Resources Information Center

Bots, Pieter W. G.; Hofstede, Gert Jan

2004-01-01

The TAKEOVER TRIO is a business game about a takeover. Three stakeholders (mother company, ailing daughter company, and potential buying company) must agree on a plan to prevent compulsory liquidation of the daughter. The primary learning objective is to make participants aware of the effect of negotiation process parameters on its outcome.…

Excess Maternal Transmission of Markers in TCOF1 Among Cleft Palate Case-Parent Trios From Three Populations

PubMed Central

Sull, Jae Woong; Liang, Kung-Yee; Hetmanski, Jacqueline B.; Fallin, M. Daniele; Ingersoll, Roxanne G.; Park, Ji Wan; Wu-Chou, Yah-Huei; Chen, Philip K.; Chong, Samuel S.; Cheah, Felicia; Yeow, Vincent; Park, Beyoung Yun; Jee, Sun Ha; Jabs, Ethylin W.; Redett, Richard; Scott, Alan F.; Beaty, Terri H.

2009-01-01

Isolated cleft palate is among the most common human birth defects. The TCOF1 gene has been suggested as a candidate gene for cleft palate based on animal models. This study tests for association between markers in TCOF1 and isolated, nonsyndromic cleft palate using a case-parent trio design considering parent-of-origin effects. Case-parent trios from three populations (comprising a total of 81 case-parent trios) were genotyped for single nucleotide polymorphisms (SNPs) in the TCOF1 gene. We used the transmission disequilibrium test and the transmission asymmetry test on individual SNPs. When all trios were combined, the odds ratio for transmission of the minor allele, OR(transmission), was significant for SNP rs15251 (OR = 2.88, P = 0.007), as well as rs2255796 and rs2569062 (OR = 2.08, P = 0.03; OR = 2.43, P = 0.041; respectively) when parent of origin was not considered. The transmission asymmetry test also revealed one SNP (rs15251) showing excess maternal transmission significant at the P = 0.005 level (OR = 6.50). Parent-of-origin effects were assessed using the parent-of-origin likelihood ratio test on both SNPs and haplotypes. While the parent-of-origin likelihood ratio test was only marginally significant for this SNP (P = 0.136), analysis of haplotypes of rs2255796 and rs15251 suggested excess maternal transmission. Therefore, these data suggest TCOF1 may influence risk of cleft palate through a parent-of-origin effect. PMID:18688869

Performance of the SNPforID 52 SNP-plex assay in paternity testing.

PubMed

Børsting, Claus; Sanchez, Juan J; Hansen, Hanna E; Hansen, Anders J; Bruun, Hanne Q; Morling, Niels

2008-09-01

The performance of a multiplex assay with 52 autosomal single nucleotide polymorphisms (SNPs) developed for human identification was tested on 124 mother-child-father trios. The typical paternity indices (PIs) were 10(5)-10(6) for the trios and 10(3)-10(4) for the child-father duos. Using the SNP profiles from the randomly selected trios and 700 previously typed individuals, a total of 83,096 comparisons between mother, child and an unrelated man were performed. On average, 9-10 mismatches per comparison were detected. Four mismatches were genetic inconsistencies and 5-6 mismatches were opposite homozygosities. In only two of the 83,096 comparisons did an unrelated man match perfectly to a mother-child duo, and in both cases the PI of the true father was much higher than the PI of the unrelated man. The trios were also typed for 15 short tandem repeats (STRs) and seven variable number of tandem repeats (VNTRs). The typical PIs based on 15 STRs or seven VNTRs were 5-50 times higher than the typical PIs based on 52 SNPs. Six mutations in tandem repeats were detected among the randomly selected trios. In contrast, there was not found any mutations in the SNP loci. The results showed that the 52 SNP-plex assay is a very useful alternative to currently used methods in relationship testing. The usefulness of SNP markers with low mutation rates in paternity and immigration casework is discussed.

Analysis of exome sequence in 604 trios for recessive genotypes in schizophrenia

PubMed Central

Rees, E; Kirov, G; Walters, J T; Richards, A L; Howrigan, D; Kavanagh, D H; Pocklington, A J; Fromer, M; Ruderfer, D M; Georgieva, L; Carrera, N; Gormley, P; Palta, P; Williams, H; Dwyer, S; Johnson, J S; Roussos, P; Barker, D D; Banks, E; Milanova, V; Rose, S A; Chambert, K; Mahajan, M; Scolnick, E M; Moran, J L; Tsuang, M T; Glatt, S J; Chen, W J; Hwu, H-G; Faraone, Stephen V; Roe, Cheri A; Chandler, Sharon D; Liu, Chih-Min; Liu, Chen-Chung; Yeh, Ling-Ling; Ouyang, Wen-Chen; Chan, Hung-Yu; Chen, Chun-Ying; Neale, B M; Palotie, A; Sklar, P; Purcell, S M; McCarroll, S A; Holmans, P; Owen, M J; O'Donovan, M C

2015-01-01

Genetic associations involving both rare and common alleles have been reported for schizophrenia but there have been no systematic scans for rare recessive genotypes using fully phased trio data. Here, we use exome sequencing in 604 schizophrenia proband–parent trios to investigate the role of recessive (homozygous or compound heterozygous) nonsynonymous genotypes in the disorder. The burden of recessive genotypes was not significantly increased in probands at either a genome-wide level or in any individual gene after adjustment for multiple testing. At a system level, probands had an excess of nonsynonymous compound heterozygous genotypes (minor allele frequency, MAF ⩽1%) in voltage-gated sodium channels (VGSCs; eight in probands and none in parents, P=1.5 × 10−4). Previous findings of multiple de novo loss-of-function mutations in this gene family, particularly SCN2A, in autism and intellectual disability provide biological and genetic plausibility for this finding. Pointing further to the involvement of VGSCs in schizophrenia, we found that these genes were enriched for nonsynonymous mutations (MAF ⩽0.1%) in cases genotyped using an exome array, (5585 schizophrenia cases and 8103 controls), and that in the trios data, synaptic proteins interacting with VGSCs were also enriched for both compound heterozygosity (P=0.018) and de novo mutations (P=0.04). However, we were unable to replicate the specific association with compound heterozygosity at VGSCs in an independent sample of Taiwanese schizophrenia trios (N=614). We conclude that recessive genotypes do not appear to make a substantial contribution to schizophrenia at a genome-wide level. Although multiple lines of evidence, including several from this study, suggest that rare mutations in VGSCs contribute to the disorder, in the absence of replication of the original findings regarding compound heterozygosity, this conclusion requires evaluation in a larger sample of trios. PMID:26196440

TRIO: Turbulent Response in Oxygen

NASA Technical Reports Server (NTRS)

Hecht, J. H.; Clemmons, J. H.; Katz, N.; Ben-Ami, M.; Carranza, P. A.; Larsen, M.

2005-01-01

This project was designed to build on the results from the successful launch of the Turbulent Oxygen Mixing Experiment (TOMEX) mother-daughter (instrumented and chemical-release) payload (21.126) that was launched in October 2000 from the White Sands Missile Range. The overall science objective was to investigate the evolution of the atmospheric response. at altitudes between 80 and 120 km, to the presence of unstable regions with vertical scales of the order of 1 to 10 km. TRIO was designed to use Na lidar measurements from the MAUUMALT observation on MAUI with a launch of a payload from Pacific Missile Range Facility (PMRF), located on Kauai. During this project, Aerospace participated in a Mission Initiation Conference. put together a science requirements document. performed a site visit to PMRF. prepared a CDR document. and developed a production and calibration procedure for one of the payload instruments. the 3-channel photometer. Unfortunately. NASA decided to terminate the program because of unforeseen (by NASA) range costs. This CDR document represents our view of this project at termination and provides a roadmap to perform this experiment should it be proposed again.

Excess maternal transmission of markers in TCOF1 among cleft palate case-parent trios from three populations.

PubMed

Sull, Jae Woong; Liang, Kung-Yee; Hetmanski, Jacqueline B; Fallin, M Daniele; Ingersoll, Roxanne G; Park, Ji Wan; Wu-Chou, Yah-Huei; Chen, Philip K; Chong, Samuel S; Cheah, Felicia; Yeow, Vincent; Park, Beyoung Yun; Jee, Sun Ha; Jabs, Ethylin W; Redett, Richard; Scott, Alan F; Beaty, Terri H

2008-09-15

Isolated cleft palate is among the most common human birth defects. The TCOF1 gene has been suggested as a candidate gene for cleft palate based on animal models. This study tests for association between markers in TCOF1 and isolated, nonsyndromic cleft palate using a case-parent trio design considering parent-of-origin effects. Case-parent trios from three populations (comprising a total of 81 case-parent trios) were genotyped for single nucleotide polymorphisms (SNPs) in the TCOF1 gene. We used the transmission disequilibrium test and the transmission asymmetry test on individual SNPs. When all trios were combined, the odds ratio for transmission of the minor allele, OR(transmission), was significant for SNP rs15251 (OR = 2.88, P = 0.007), as well as rs2255796 and rs2569062 (OR = 2.08, P = 0.03; OR = 2.43, P = 0.041; respectively) when parent of origin was not considered. The transmission asymmetry test also revealed one SNP (rs15251) showing excess maternal transmission significant at the P = 0.005 level (OR = 6.50). Parent-of-origin effects were assessed using the parent-of-origin likelihood ratio test on both SNPs and haplotypes. While the parent-of-origin likelihood ratio test was only marginally significant for this SNP (P = 0.136), analysis of haplotypes of rs2255796 and rs15251 suggested excess maternal transmission. Therefore, these data suggest TCOF1 may influence risk of cleft palate through a parent-of-origin effect. Copyright 2008 Wiley-Liss, Inc.

Confirming genes influencing risk to cleft lip with/without cleft palate in a case-parent trio study.

PubMed

Beaty, T H; Taub, M A; Scott, A F; Murray, J C; Marazita, M L; Schwender, H; Parker, M M; Hetmanski, J B; Balakrishnan, P; Mansilla, M A; Mangold, E; Ludwig, K U; Noethen, M M; Rubini, M; Elcioglu, N; Ruczinski, I

2013-07-01

A collection of 1,108 case-parent trios ascertained through an isolated, nonsyndromic cleft lip with or without cleft palate (CL/P) was used to replicate the findings from a genome-wide association study (GWAS) conducted by Beaty et al. (Nat Genet 42:525-529, 2010), where four different genes/regions were identified as influencing risk to CL/P. Tagging SNPs for 33 different genes were genotyped (1,269 SNPs). All four of the genes originally identified as showing genome-wide significance (IRF6, ABCA4 and MAF, plus the 8q24 region) were confirmed in this independent sample of trios (who were primarily of European and Southeast Asian ancestry). In addition, eight genes classified as 'second tier' hits in the original study (PAX7, THADA, COL8A1/FILIP1L, DCAF4L2, GADD45G, NTN1, RBFOX3 and FOXE1) showed evidence of linkage and association in this replication sample. Meta-analysis between the original GWAS trios and these replication trios showed PAX7, COL8A1/FILIP1L and NTN1 achieved genome-wide significance. Tests for gene-environment interaction between these 33 genes and maternal smoking found evidence for interaction with two additional genes: GRID2 and ELAVL2 among European mothers (who had a higher rate of smoking than Asian mothers). Formal tests for gene-gene interaction (epistasis) failed to show evidence of statistical interaction in any simple fashion. This study confirms that many different genes influence risk to CL/P.

Confirming genes influencing risk to cleft lip with/without cleft palate in a case-parent trio study

PubMed Central

Beaty, TH; Taub, MA; Scott, AF; Murray, JC; Marazita, ML; Schwender, H; Parker, MM; Hetmanski, JB; Balakrishnan, P; Mansilla, MA; Mangold, E; Ludwig, KU; Noethen, MM; Rubini, M; Elcioglu, N; Ruczinski, I

2013-01-01

A collection of 1,108 case-parent trios ascertained through an isolated, non-syndromic cleft lip with or without cleft palate (CL/P) was used to replicate the findings from a genome-wide association study (GWAS) conducted by Beaty et al. (2010) where four different genes/regions were identified as influencing risk to CL/P. Tagging SNPs for 33 different genes were genotyped (1,269 SNPs). All four of the genes originally identified as showing genome-wide significance (IRF6, ABCA4 and MAF, plus the 8q24 region) were confirmed in this independent sample of trios (who were primarily of European and Southeast Asian ancestry). In addition, eight genes classified as ‘second tier’ hits in the original study (PAX7, THADA, COL8A1/FILIP1L, DCAF4L2, GADD45G, NTN1, RBFOX3 and FOXE1) showed evidence of linkage and association in this replication sample. Meta-analysis between the original GWAS trios and these replication trios showed PAX7, COL8A1/FILIP1L and NTN1 achieved genome-wide significance. Tests for gene-environment interaction between these 33 genes and maternal smoking found evidence for interaction with two additional genes: GRID2 and ELAVL2 among European mothers (who had a higher rate of smoking than Asian mothers). Formal tests for gene-gene interaction (epistasis) failed to show evidence of statistical interaction in any simple fashion. This study confirms that many different genes influence risk to CL/P. PMID:23512105

The Akuriyo of Surinam. A Case of Emergence from Isolation. IWGIA Document 27.

ERIC Educational Resources Information Center

Kloos, Peter

In 1968, the Amerindian nomads of southeast Surinam, South America known as the Akuriyo were rediscovered by the West Indies Mission through the Wayana and Trio Indians with whom the Mission worked in Surinam. Although the Akuriyo had always been suspicious of other peoples, they soon realized the missionaries and the Trio meant them no harm.…

TRIO: Burst Buffer Based I/O Orchestration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, Teng; Oral, H Sarp; Pritchard, Michael

The growing computing power on leadership HPC systems is often accompanied by ever-escalating failure rates. Checkpointing is a common defensive mechanism used by scientific applications for failure recovery. However, directly writing the large and bursty checkpointing dataset to parallel filesystem can incur significant I/O contention on storage servers. Such contention in turn degrades the raw bandwidth utilization of storage servers and prolongs the average job I/O time of concurrent applications. Recently burst buffer has been proposed as an intermediate layer to absorb the bursty I/O traffic from compute nodes to storage backend. But an I/O orchestration mechanism is still desiredmore » to efficiently move checkpointing data from bursty buffers to storage backend. In this paper, we propose a burst buffer based I/O orchestration framework, named TRIO, to intercept and reshape the bursty writes for better sequential write traffic to storage severs. Meanwhile, TRIO coordinates the flushing orders among concurrent burst buffers to alleviate the contention on storage server bandwidth. Our experimental results reveal that TRIO can deliver 30.5% higher bandwidth and reduce the average job I/O time by 37% on average for data-intensive applications in various checkpointing scenarios.« less

Space Station Astronauts Make Safe Landing on This Week @NASA – September 11, 2015

NASA Image and Video Library

2015-09-11

Aboard the International Space Station, the Expedition 45 crew – including new Commander Scott Kelly and Kjell Lindgren of NASA, said goodbye to Gennady Padalka of the Russian Federal Space Agency, Andreas Mogensen of ESA (European Space Agency) and Aidyn Aimbetov of the Kazakh Space Agency (Kazcosmos) as the trio climbed aboard their Soyuz spacecraft for the return trip to Earth. The Soyuz landed safely in Kazakhstan on Sept. 11 Eastern time, Sept. 12 in Kazakhstan -- closing out a 168-day mission for Padalka and an 8-day stay on the station for Mogensen and Aimbetov. Also, First Orion crew module segments welded, SLS Launch Vehicle Stage Adapter, New Ceres imagery, New Horizons update, 9/11 tribute and National Preparedness Month!

Effects of Breeding Configuration on Maternal and Weanling Behavior in Laboratory Mice.

PubMed

Braden, Gillian C; Rasmussen, Skye; Monette, Sebastien; Tolwani, Ravi J

2017-07-01

Although numerous studies have evaluated the effect of housing density on the wellbeing of laboratory mice, little is known about the effect of breeding configuration on mouse behavior. The 8th edition of the Guide for the Care and Use of Laboratory Animals lists the recommended minimal floor area per animal for a female mouse and her litter as 51 in.2 We sought to determine the effects of pair, trio, and harem breeding configurations on the maternal and weanling behavior of C57BL/6J (B6) and 129S6/SvEvTac (129) mice on the basis of nest scores and performance in pup retrieval tests, open-field test (OFT), elevated plus maze, and tail suspension test; we concurrently evaluated cage microenvironment, reproductive indices, and anatomic and clinical pathology. Harem breeding configurations enhanced B6 maternal behaviors as evidenced by significantly shorter pup retrieval times. Trio- and harem-raised B6 weanlings showed increased exploratory behaviors, as evidenced by greater time spent in the center of the OFT, when compared with pair-raised B6 mice. Conversely, breeding configuration did not alter pup retrieval times for 129 mice, and on the day of weaning trio- and harem-raised 129 mice demonstrated increased anxiety-like behavior, as evidenced by greater time spent in the periphery of the OFT, when compared with pair-raised counterparts. Behavioral differences were not noted on subsequent days for either strain. Trio- and harem-raised B6 and 129 weanling mice had significantly higher weaning weights than weanlings raised in a pair breeding configuration. Trio and harem breeding in a standard 67-in.2 shoebox cage did not detrimentally affect the evaluated welfare parameters in either C57BL/6J or 129S6/SvEvTac mice.

Disease concepts and treatment by tribal healers of an Amazonian forest culture.

PubMed

Herndon, Christopher N; Uiterloo, Melvin; Uremaru, Amasina; Plotkin, Mark J; Emanuels-Smith, Gwendolyn; Jitan, Jeetendra

2009-10-12

The extensive medicinal plant knowledge of Amazonian tribal peoples is widely recognized in the scientific literature and celebrated in popular lore. Despite this broad interest, the ethnomedical systems and knowledge of disease which guide indigenous utilization of botanical diversity for healing remain poorly characterized and understood. No study, to our knowledge, has attempted to directly examine patterns of actual disease recognition and treatment by healers of an Amazonian indigenous culture. The establishment of traditional medicine clinics, operated and directed by elder tribal shamans in two remote Trio villages of the Suriname rainforest, presented a unique investigational opportunity. Quantitative analysis of clinic records from both villages permitted examination of diseases treated over a continuous period of four years. Cross-cultural comparative translations were articulated of recorded disease conditions through ethnographic interviews of elder Trio shamans and a comprehensive atlas of indigenous anatomical nomenclature was developed. 20,337 patient visits within the period 2000 to 2004 were analyzed. 75 disease conditions and 127 anatomical terms are presented. Trio concepts of disease and medical practices are broadly examined within the present and historical state of their culture. The findings of this investigation support the presence of a comprehensive and highly formalized ethnomedical institution within Trio culture with attendant health policy and conservation implications.

The TRIO Framework: Conceptual insights into family caregiver involvement and influence throughout cancer treatment decision-making.

PubMed

Laidsaar-Powell, Rebekah; Butow, Phyllis; Charles, Cathy; Gafni, Amiram; Entwistle, Vikki; Epstein, Ronald; Juraskova, Ilona

2017-11-01

Family caregivers are regularly involved in cancer consultations and treatment decision-making (DM). Yet there is limited conceptual description of caregiver influence/involvement in DM. To address this, an empirically-grounded conceptual framework of triadic DM (TRIO Framework) and corresponding graphical aid (TRIO Triangle) were developed. Jabareen's model for conceptual framework development informed multiple phases of development/validation, incorporation of empirical research and theory, and iterative revisions by an expert advisory group. Findings coalesced into six empirically-grounded conceptual insights: i) Caregiver influence over a decision is variable amongst different groups; ii) Caregiver influence is variable within the one triad over time; iii) Caregivers are involved in various ways in the wider DM process; iv) DM is not only amongst three, but can occur among wider social networks; v) Many factors may affect the form and extent of caregiver involvement in DM; vi) Caregiver influence over, and involvement in, DM is linked to their everyday involvement in illness care/management. The TRIO Framework/Triangle may serve as a useful guide for future empirical, ethical and/or theoretical work. This Framework can deepen clinicians's and researcher's understanding of the diverse and varying scope of caregiver involvement and influence in DM. Copyright © 2017 Elsevier B.V. All rights reserved.

[Tuberculosis among Trio-Indians in Surinam].

PubMed

van Crevel, R; van Doorninck, D J; van Ams, J E; Fat, H Tjon; Vreden, S G; van der Meer, J M

2004-02-28

Evaluation of the extent and possible causes of the increased incidence of tuberculosis among Amazonian Indians in Surinam. Descriptive. In two cross-sectional surveys in 1998 and 2000, the inhabitants of Kwamalasamutu, a village of Trio-Indians in Surinam, were examined for the presence of active and latent tuberculosis. Previous cases from the period 1995-2000 were evaluated retrospectively by consulting individual physicians and the archives of the 'Medische Zending' (Medical Mission), the 'Diakonessenhuis' hospital, the clinic for pulmonary diseases, and the Central Laboratory. Family ties and other factors that might be associated with tuberculosis were examined. Spoligotyping was done on all patient isolates. Between 1995 and 2000, active tuberculosis was diagnosed in 25 Indians from Kwamalasamutu, equal to 4.2 cases/1000 person-years (95% CI: 2.7-6.1). Tuberculin skin tests were positive in 105/733 Indians (14.3%). Cases of tuberculosis were found predominantly within certain families, who were genetically related. Spoligotyping of 5 Mycobacterium tuberculosis isolates from Trio-Indians showed unique patterns, which were also found in 34 isolates from elsewhere in Surinam. Tuberculosis was relatively common among Trio-Indians, clustering in certain families. This isolated tribe may have a genetic predisposition for tuberculosis, but their lifestyle and limited access to health care certainly play a role as well.

Assessing and comparison of different machine learning methods in parent-offspring trios for genotype imputation.

PubMed

Mikhchi, Abbas; Honarvar, Mahmood; Kashan, Nasser Emam Jomeh; Aminafshar, Mehdi

2016-06-21

Genotype imputation is an important tool for prediction of unknown genotypes for both unrelated individuals and parent-offspring trios. Several imputation methods are available and can either employ universal machine learning methods, or deploy algorithms dedicated to infer missing genotypes. In this research the performance of eight machine learning methods: Support Vector Machine, K-Nearest Neighbors, Extreme Learning Machine, Radial Basis Function, Random Forest, AdaBoost, LogitBoost, and TotalBoost compared in terms of the imputation accuracy, computation time and the factors affecting imputation accuracy. The methods employed using real and simulated datasets to impute the un-typed SNPs in parent-offspring trios. The tested methods show that imputation of parent-offspring trios can be accurate. The Random Forest and Support Vector Machine were more accurate than the other machine learning methods. The TotalBoost performed slightly worse than the other methods.The running times were different between methods. The ELM was always most fast algorithm. In case of increasing the sample size, the RBF requires long imputation time.The tested methods in this research can be an alternative for imputation of un-typed SNPs in low missing rate of data. However, it is recommended that other machine learning methods to be used for imputation. Copyright © 2016 Elsevier Ltd. All rights reserved.

Disease concepts and treatment by tribal healers of an Amazonian forest culture

PubMed Central

Herndon, Christopher N; Uiterloo, Melvin; Uremaru, Amasina; Plotkin, Mark J; Emanuels-Smith, Gwendolyn; Jitan, Jeetendra

2009-01-01

Background The extensive medicinal plant knowledge of Amazonian tribal peoples is widely recognized in the scientific literature and celebrated in popular lore. Despite this broad interest, the ethnomedical systems and knowledge of disease which guide indigenous utilization of botanical diversity for healing remain poorly characterized and understood. No study, to our knowledge, has attempted to directly examine patterns of actual disease recognition and treatment by healers of an Amazonian indigenous culture. Methods The establishment of traditional medicine clinics, operated and directed by elder tribal shamans in two remote Trio villages of the Suriname rainforest, presented a unique investigational opportunity. Quantitative analysis of clinic records from both villages permitted examination of diseases treated over a continuous period of four years. Cross-cultural comparative translations were articulated of recorded disease conditions through ethnographic interviews of elder Trio shamans and a comprehensive atlas of indigenous anatomical nomenclature was developed. Results 20,337 patient visits within the period 2000 to 2004 were analyzed. 75 disease conditions and 127 anatomical terms are presented. Trio concepts of disease and medical practices are broadly examined within the present and historical state of their culture. Conclusion The findings of this investigation support the presence of a comprehensive and highly formalized ethnomedical institution within Trio culture with attendant health policy and conservation implications. PMID:19821968

Culture and the Contagion of Conflict: Social Science and Computational Approaches

DTIC Science & Technology

2015-08-05

research , special, group study, etc. 3. DATES COVERED. Indicate the time during which the work was performed and the report was written, e.g., Jun 1997...to the Journal of Experimental Social Psychology for review. Our other research (Shytenberg et al., under revision, European Journal of Social... experimental trios that comprise various combinations of group membership. For example, for some trios, Persons B and C were friends who came to the

The Wealth of the Body: Trade Relations, Objects, and Personhood in Northeastern Amazonia

PubMed Central

Grotti, Vanessa

2016-01-01

This article is an analysis of trade among the Trio (Suriname), and their relationship with objects and persons in their quest for manufactured goods. Based on data mostly collected in the Trio village of Tëpu in southern Suriname, it discusses trade from the point of view of Amerindian sociality, with regard to the nature of the interpersonal relations involved. I examine trade through the prism of an Amerindian understanding of personhood, the body and materiality, and show how these relationships tend to be fabricated over a lifetime, eventually becoming an integral and material part of the actors involved. This is manifested in the way Trio social space is constructed and inhabited as an extension of the body, and how objects acquired through trade come to elicit narratives of past exploits and travels to distant spheres of alterity. [Brazil, Guyana, indigenous people, social anthropology, Surinam] PMID:27175223

Finish line distinctness and accuracy in 7 intraoral scanners versus conventional impression: an in vitro descriptive comparison.

PubMed

Nedelcu, Robert; Olsson, Pontus; Nyström, Ingela; Thor, Andreas

2018-02-23

Several studies have evaluated accuracy of intraoral scanners (IOS), but data is lacking regarding variations between IOS systems in the depiction of the critical finish line and the finish line accuracy. The aim of this study was to analyze the level of finish line distinctness (FLD), and finish line accuracy (FLA), in 7 intraoral scanners (IOS) and one conventional impression (IMPR). Furthermore, to assess parameters of resolution, tessellation, topography, and color. A dental model with a crown preparation including supra and subgingival finish line was reference-scanned with an industrial scanner (ATOS), and scanned with seven IOS: 3M, CS3500 and CS3600, DWIO, Omnicam, Planscan and Trios. An IMPR was taken and poured, and the model was scanned with a laboratory scanner. The ATOS scan was cropped at finish line and best-fit aligned for 3D Compare Analysis (Geomagic). Accuracy was visualized, and descriptive analysis was performed. All IOS, except Planscan, had comparable overall accuracy, however, FLD and FLA varied substantially. Trios presented the highest FLD, and with CS3600, the highest FLA. 3M, and DWIO had low overall FLD and low FLA in subgingival areas, whilst Planscan had overall low FLD and FLA, as well as lower general accuracy. IMPR presented high FLD, except in subgingival areas, and high FLA. Trios had the highest resolution by factor 1.6 to 3.1 among IOS, followed by IMPR, DWIO, Omnicam, CS3500, 3M, CS3600 and Planscan. Tessellation was found to be non-uniform except in 3M and DWIO. Topographic variation was found for 3M and Trios, with deviations below +/- 25 μm for Trios. Inclusion of color enhanced the identification of the finish line in Trios, Omnicam and CS3600, but not in Planscan. There were sizeable variations between IOS with both higher and lower FLD and FLA than IMPR. High FLD was more related to high localized finish line resolution and non-uniform tessellation, than to high overall resolution. Topography variations were low. Color improved finish line identification in some IOS. It is imperative that clinicians critically evaluate the digital impression, being aware of varying technical limitations among IOS, in particular when challenging subgingival conditions apply.

Trio’s Rho-specific GEF domain is the missing Gαq effector in C. elegans

PubMed Central

Williams, Stacey L.; Lutz, Susanne; Charlie, Nicole K.; Vettel, Christiane; Ailion, Michael; Coco, Cassandra; Tesmer, John J.G.; Jorgensen, Erik M.; Wieland, Thomas; Miller, Kenneth G.

2007-01-01

The Gαq pathway is essential for animal life and is a central pathway for driving locomotion, egg laying, and growth in Caenorhabditis elegans, where it exerts its effects through EGL-8 (phospholipase Cβ [PLCβ]) and at least one other effector. To find the missing effector, we performed forward genetic screens to suppress the slow growth and hyperactive behaviors of mutants with an overactive Gαq pathway. Four suppressor mutations disrupted the Rho-specific guanine-nucleotide exchange factor (GEF) domain of UNC-73 (Trio). The mutations produce defects in neuronal function, but not neuronal development, that cause sluggish locomotion similar to animals lacking EGL-8 (PLCβ). Strains containing null mutations in both EGL-8 (PLCβ) and UNC-73 (Trio RhoGEF) have strong synthetic phenotypes that phenocopy the arrested growth and near-complete paralysis of Gαq-null mutants. Using cell-based and biochemical assays, we show that activated C. elegans Gαq synergizes with Trio RhoGEF to activate RhoA. Activated Gαq and Trio RhoGEF appear to be part of a signaling complex, because they coimmunoprecipitate when expressed together in cells. Our results show that Trio’s Rho-specific GEF domain is a major Gαq effector that, together with PLCβ, mediates the Gαq signaling that drives the locomotion, egg laying, and growth of the animal. PMID:17942708

Detection of de novo single nucleotide variants in offspring of atomic-bomb survivors close to the hypocenter by whole-genome sequencing.

PubMed

Horai, Makiko; Mishima, Hiroyuki; Hayashida, Chisa; Kinoshita, Akira; Nakane, Yoshibumi; Matsuo, Tatsuki; Tsuruda, Kazuto; Yanagihara, Katsunori; Sato, Shinya; Imanishi, Daisuke; Imaizumi, Yoshitaka; Hata, Tomoko; Miyazaki, Yasushi; Yoshiura, Koh-Ichiro

2018-03-01

Ionizing radiation released by the atomic bombs at Hiroshima and Nagasaki, Japan, in 1945 caused many long-term illnesses, including increased risks of malignancies such as leukemia and solid tumours. Radiation has demonstrated genetic effects in animal models, leading to concerns over the potential hereditary effects of atomic bomb-related radiation. However, no direct analyses of whole DNA have yet been reported. We therefore investigated de novo variants in offspring of atomic-bomb survivors by whole-genome sequencing (WGS). We collected peripheral blood from three trios, each comprising a father (atomic-bomb survivor with acute radiation symptoms), a non-exposed mother, and their child, none of whom had any past history of haematological disorders. One trio of non-exposed individuals was included as a control. DNA was extracted and the numbers of de novo single nucleotide variants in the children were counted by WGS with sequencing confirmation. Gross structural variants were also analysed. Written informed consent was obtained from all participants prior to the study. There were 62, 81, and 42 de novo single nucleotide variants in the children of atomic-bomb survivors, compared with 48 in the control trio. There were no gross structural variants in any trio. These findings are in accord with previously published results that also showed no significant genetic effects of atomic-bomb radiation on second-generation survivors.

Stereo ENA Imaging of the Ring Current and Multi-point Measurements of Suprathermal Particles and Magnetic Fields by TRIO-CINEMA

NASA Astrophysics Data System (ADS)

Lin, R. P.; Sample, J. G.; Immel, T. J.; Lee, D.; Horbury, T. S.; Jin, H.; SEON, J.; Wang, L.; Roelof, E. C.; Lee, E.; Parks, G. K.; Vo, H.

2012-12-01

The TRIO (Triplet Ionospheric Observatory) - CINEMA (Cubesat for Ions, Neutrals, Electrons, & Magnetic fields) mission consists of three identical 3-u cubesats to provide high sensitivity, high cadence, stereo measurements of Energetic Neutral Atoms (ENAs) from the Earth's ring current with ~1 keV FWHM energy resolution from ~4 to ~200 keV, as well as multi-point in situ measurements of magnetic fields and suprathermal electrons (~2 -200 keV) and ions (~ 4 -200 keV) in the auroral and ring current precipitation regions in low Earth orbit (LEO). A new Suprathermal Electron, Ion, Neutral (STEIN) instrument, using a 32-pixel silicon semiconductor detector with an electrostatic deflection system to separate ENAs from ions and from electrons below 30 keV, will sweep over most of the sky every 15 s as the spacecraft spins at 4 rpm. In addition, inboard and outboard (on an extendable 1m boom) miniature magnetoresistive sensor magnetometers will provide high cadence 3-axis magnetic field measurements. An S-band transmitter will be used to provide ~8 kbps orbit-average data downlink to the ~11m diameter antenna of the Berkeley Ground Station.The first CINEMA (funded by NSF) is scheduled for launch on August 14, 2012 into a 65 deg. inclination LEO. Two more identical CINEMAs are being developed by Kyung Hee University (KHU) in Korea under the World Class University (WCU) program, for launch in November 2012 into a Sun-synchronous LEO to form TRIO-CINEMA. A fourth CINEMA is being developed for a 2013 launch into LEO. This LEO constellation of nanosatellites will provide unique measurements highly complementary to NASA's RBSP and THEMIS missions. Furthermore, CINEMA's development of miniature particle and magnetic field sensors, and cubesat-size spinning spacecraft may be important for future constellation space missions. Initial results from the first CINEMA will be presented if available.

A gradient-boosting approach for filtering de novo mutations in parent-offspring trios.

PubMed

Liu, Yongzhuang; Li, Bingshan; Tan, Renjie; Zhu, Xiaolin; Wang, Yadong

2014-07-01

Whole-genome and -exome sequencing on parent-offspring trios is a powerful approach to identifying disease-associated genes by detecting de novo mutations in patients. Accurate detection of de novo mutations from sequencing data is a critical step in trio-based genetic studies. Existing bioinformatic approaches usually yield high error rates due to sequencing artifacts and alignment issues, which may either miss true de novo mutations or call too many false ones, making downstream validation and analysis difficult. In particular, current approaches have much worse specificity than sensitivity, and developing effective filters to discriminate genuine from spurious de novo mutations remains an unsolved challenge. In this article, we curated 59 sequence features in whole genome and exome alignment context which are considered to be relevant to discriminating true de novo mutations from artifacts, and then employed a machine-learning approach to classify candidates as true or false de novo mutations. Specifically, we built a classifier, named De Novo Mutation Filter (DNMFilter), using gradient boosting as the classification algorithm. We built the training set using experimentally validated true and false de novo mutations as well as collected false de novo mutations from an in-house large-scale exome-sequencing project. We evaluated DNMFilter's theoretical performance and investigated relative importance of different sequence features on the classification accuracy. Finally, we applied DNMFilter on our in-house whole exome trios and one CEU trio from the 1000 Genomes Project and found that DNMFilter could be coupled with commonly used de novo mutation detection approaches as an effective filtering approach to significantly reduce false discovery rate without sacrificing sensitivity. The software DNMFilter implemented using a combination of Java and R is freely available from the website at http://humangenome.duke.edu/software. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

The accuracy of the CAD system using intraoral and extraoral scanners for designing of fixed dental prostheses.

PubMed

Shimizu, Sakura; Shinya, Akikazu; Kuroda, Soichi; Gomi, Harunori

2017-07-26

The accuracy of prostheses affects clinical success and is, in turn, affected by the accuracy of the scanner and CAD programs. Thus, their accuracy is important. The first aim of this study was to evaluate the accuracy of an intraoral scanner with active triangulation (Cerec Omnicam), an intraoral scanner with a confocal laser (3Shape Trios), and an extraoral scanner with active triangulation (D810). The second aim of this study was to compare the accuracy of the digital crowns designed with two different scanner/CAD combinations. The accuracy of the intraoral scanners and extraoral scanner was clinically acceptable. Marginal and internal fit of the digital crowns fabricated using the intraoral scanner and CAD programs were inferior to those fabricated using the extraoral scanner and CAD programs.

Interleukin 1 beta gene and risk of schizophrenia: detailed case-control and family-based studies and an updated meta-analysis.

PubMed

Shibuya, Masako; Watanabe, Yuichiro; Nunokawa, Ayako; Egawa, Jun; Kaneko, Naoshi; Igeta, Hirofumi; Someya, Toshiyuki

2014-01-01

Interleukin-1 beta (IL-1β) has been implicated in the pathophysiology of schizophrenia. To assess whether the IL1B gene confers increased susceptibility to schizophrenia, we conducted case-control and family-based studies and an updated meta-analysis. We tested the association between IL1B and schizophrenia in 1229 case-control and 112 trio samples using 12 markers, including common tagging single nucleotide variations (SNVs) and a rare non-synonymous variation detected by resequencing the coding regions. We also performed a meta-analysis of rs16944 using a total of 8724 case-control and 201 trio samples from 16 independent populations. We found no significant associations between any of the 12 SNVs examined and schizophrenia in either case-control or trio samples. Moreover, our meta-analysis results showed no significant association between the common SNV, rs16944, and schizophrenia. The present study does not support a role for IL1B in schizophrenia susceptibility.

[Precision of digital impressions with TRIOS under simulated intraoral impression taking conditions].

PubMed

Yang, Xin; Sun, Yi-fei; Tian, Lei; Si, Wen-jie; Feng, Hai-lan; Liu, Yi-hong

2015-02-18

To evaluate the precision of digital impressions taken under simulated clinical impression taking conditions with TRIOS and to compare with the precision of extraoral digitalizations. Six #14-#17 epoxy resin dentitions with extracted #16 tooth preparations embedded were made. For each artificial dentition, (1)a silicone rubber impression was taken with individual tray, poured with type IV plaster,and digitalized with 3Shape D700 model scanner for 10 times; (2) fastened to a dental simulator, 10 digital impressions for each were taken with 3Shape TRIOS intraoral scanner. To assess the precision, best-fit algorithm and 3D comparison were conducted between repeated scan models pairwise by Geomagic Qualify 12.0, exported as averaged errors (AE) and color-coded diagrams. Non-parametric analysis was performed to compare the precisions of digital impressions and model images. The color-coded diagrams were used to show the deviations distributions. The mean of AE for digital impressions was 7.058 281 μm, which was greater than that of 4.092 363 μm for the model images (P<0.05). However, the means and medians of AE for digital impressions were no more than 10 μm, which meant that the consistency between the digital impressions was good. The deviations distribution was uniform in the model images,while nonuniform in the digital impressions with greater deviations lay mainly around the shoulders and interproximal surfaces. Digital impressions with TRIOS are of good precision and up to the clinical standard. Shoulders and interproximal surfaces scanning are more difficult.

Detection of Parent-of-Origin Effects Using General Pedigree Data

PubMed Central

Zhou, Ji-Yuan; Ding, Jie; Fung, Wing K.; Lin, Shili

2010-01-01

Genomic imprinting is an important epigenetic factor in complex traits study, which has generally been examined by testing for parent-of-origin effects of alleles. For a diallelic marker locus, the parental-asymmetry test (PAT) based on case-parents trios and its extensions to incomplete nuclear families (1-PAT and C-PAT) are simple and powerful for detecting parent-of-origin effects. However, these methods are suitable only for nuclear families and thus are not amenable to general pedigree data. Use of data from extended pedigrees, if available, may lead to more powerful methods than randomly selecting one two-generation nuclear family from each pedigree. In this study, we extend PAT to accommodate general pedigree data by proposing the pedigree PAT (PPAT) statistic, which uses all informative family trios from pedigrees. To fully utilize pedigrees with some missing genotypes, we further develop the Monte Carlo (MC) PPAT (MCPPAT) statistic based on MC sampling and estimation. Extensive simulations were carried out to evaluate the performance of the proposed methods. Under the assumption that the pedigrees and their associated affection patterns are randomly drawn from a population of pedigrees with at least one affected offspring, we demonstrated that MCPPAT is a valid test for parent-of-origin effects in the presence of association. Further, MCPPAT is much more powerful compared to PAT for trios or even PPAT for all informative family trios from the same pedigrees if there is missing data. Application of the proposed methods to a rheumatoid arthritis dataset further demonstrates the advantage of MCPPAT. PMID:19676055

Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep.

PubMed

Wang, Xiaolong; Liu, Jing; Niu, Yiyuan; Li, Yan; Zhou, Shiwei; Li, Chao; Ma, Baohua; Kou, Qifang; Petersen, Bjoern; Sonstegard, Tad; Huang, Xingxu; Jiang, Yu; Chen, Yulin

2018-05-25

The simplicity of the CRISPR/Cas9 system has enabled its widespread applications in generating animal models, functional genomic screening and in treating genetic and infectious diseases. However, unintended mutations produced by off-target CRISPR/Cas9 nuclease activity may lead to negative consequences. Especially, a very recent study found that gene editing can introduce hundreds of unintended mutations into the genome, and have attracted wide attention. To address the off-target concerns, urgent characterization of the CRISPR/Cas9-mediated off-target mutagenesis is highly anticipated. Here we took advantage of our previously generated gene-edited sheep and performed family trio-based whole genome sequencing which is capable of discriminating variants in the edited progenies that are inherited, naturally generated, or induced by genetic modification. Three family trios were re-sequenced at a high average depth of genomic coverage (~ 25.8×). After developing a pipeline to comprehensively analyze the sequence data for de novo single nucleotide variants, indels and structural variations from the genome; we only found a single unintended event in the form of a 2.4 kb inversion induced by site-specific double-strand breaks between two sgRNA targeting sites at the MSTN locus with a low incidence. We provide the first report on the fidelity of CRISPR-based modification for sheep genomes targeted simultaneously for gene breaks at three coding sequence locations. The trio-based sequencing approach revealed almost negligible off-target modifications, providing timely evidences of the safe application of genome editing in vivo with CRISPR/Cas9.

Application of the Trio-Tri-Star Carpal Wrist for use in a Solar Array Tracking Mechanism for the Momentum-eXchange/Electrodynamic Reboost (MXER) Tether Concept

NASA Technical Reports Server (NTRS)

Robertson, Jeffrey

2006-01-01

This paper describes the application of the Trio-Tri-Star Carpal Wrist to the Momentum Exchange Electro-Dynamic Re-boost (MXER) tether, an advanced space transportation concept being developed by the In-Space Propulsion Technology Office at NASA s Marshall Space Flight Center in Huntsville, Alabama. Dr. Joseph Bonometti and Mr. Kirk Sorensen are the principal engineers. In the paper, a brief overview of the MXER concept is given, with an emphasis on the design problem that this wrist is designed to address. The Trio-Tri-Star Carpal Wrist, a three degree of freedom parallel manipulator, invented by Dr. Stephen J. Canfield of Tennessee Tech University, is described with an overview of wrist geometry, kinematics, and stress analysis. A working model of the wrist was assembled at MSFC using Dr. Canfield s prototype to demonstrate its operation. Finally, a design description and supporting analysis of a MXER flight concept wrist is given, with recommendations for future development work.

An Allele of Sequoia Dominantly Enhances a Trio Mutant Phenotype to Influence Drosophila Larval Behavior

PubMed Central

Liebl, Eric C.

2013-01-01

The transition of Drosophila third instar larvae from feeding, photo-phobic foragers to non-feeding, photo-neutral wanderers is a classic behavioral switch that precedes pupariation. The neuronal network responsible for this behavior has recently begun to be defined. Previous genetic analyses have identified signaling components for food and light sensory inputs and neuropeptide hormonal outputs as being critical for the forager to wanderer transition. Trio is a Rho-Guanine Nucleotide Exchange Factor integrated into a variety of signaling networks including those governing axon pathfinding in early development. Sequoia is a pan-neuronally expressed zinc-finger transcription factor that governs dendrite and axon outgrowth. Using pre-pupal lethality as an endpoint, we have screened for dominant second-site enhancers of a weakly lethal trio mutant background. In these screens, an allele of sequoia has been identified. While these mutants have no obvious disruption of embryonic central nervous system architecture and survive to third instar larvae similar to controls, they retain forager behavior and thus fail to pupariate at high frequency. PMID:24376789

An allele of sequoia dominantly enhances a trio mutant phenotype to influence Drosophila larval behavior.

PubMed

Dean, Kathryn E; Fields, April; Geer, Marcus J; King, Eric C; Lynch, Brian T; Manohar, Rohan R; McCall, Julianne R; Palozola, Katherine C; Zhang, Yan; Liebl, Eric C

2013-01-01

The transition of Drosophila third instar larvae from feeding, photo-phobic foragers to non-feeding, photo-neutral wanderers is a classic behavioral switch that precedes pupariation. The neuronal network responsible for this behavior has recently begun to be defined. Previous genetic analyses have identified signaling components for food and light sensory inputs and neuropeptide hormonal outputs as being critical for the forager to wanderer transition. Trio is a Rho-Guanine Nucleotide Exchange Factor integrated into a variety of signaling networks including those governing axon pathfinding in early development. Sequoia is a pan-neuronally expressed zinc-finger transcription factor that governs dendrite and axon outgrowth. Using pre-pupal lethality as an endpoint, we have screened for dominant second-site enhancers of a weakly lethal trio mutant background. In these screens, an allele of sequoia has been identified. While these mutants have no obvious disruption of embryonic central nervous system architecture and survive to third instar larvae similar to controls, they retain forager behavior and thus fail to pupariate at high frequency.

Ab initio calculation of atomic interactions on Al(110): implications for epitaxial growth

NASA Astrophysics Data System (ADS)

Fichthorn, Kristen; Tiwary, Yogesh

2007-03-01

Using first-principles calculations based on density-functional theory, we resolved atomic interactions between adsorbed Al atoms on Al(110). Relevant pair and trio interactions were quantified. We find that pair interactions extend to the third in-channel and second cross-channel neighbor on the anisotropic (110) surface. Beyond these distances, pair interactions are negligible. The nearest-neighbor interaction in the in-channel direction is attractive, but nearest-neighbor cross-channel interaction is repulsive. While nearest-neighbor, cross-channel repulsion does not support the experimental observation of 3D hut formation in Al/Al(110) homoepitaxial growth [1], we find that trio interactions can be significant and attractive and they support cross-channel bonding. The pair and trio interactions have direct and indirect components. We have quantified the electronic and elastic components of the indirect, substrate-mediated interactions. We also probe the influence of these interactions on the energy barriers for adatom hopping. [1] F. Buatier de Mongeot, W. Zhu, A. Molle, R. Buzio, C. Boragno, U. Valbusa, E. Wang, and Z. Zhang, Phys. Rev. Lett. 91, 016102 (2003).

The Autism Simplex Collection: an international, expertly phenotyped autism sample for genetic and phenotypic analyses.

PubMed

Buxbaum, Joseph D; Bolshakova, Nadia; Brownfeld, Jessica M; Anney, Richard Jl; Bender, Patrick; Bernier, Raphael; Cook, Edwin H; Coon, Hilary; Cuccaro, Michael; Freitag, Christine M; Hallmayer, Joachim; Geschwind, Daniel; Klauck, Sabine M; Nurnberger, John I; Oliveira, Guiomar; Pinto, Dalila; Poustka, Fritz; Scherer, Stephen W; Shih, Andy; Sutcliffe, James S; Szatmari, Peter; Vicente, Astrid M; Vieland, Veronica; Gallagher, Louise

2014-01-01

There is an urgent need for expanding and enhancing autism spectrum disorder (ASD) samples, in order to better understand causes of ASD. In a unique public-private partnership, 13 sites with extensive experience in both the assessment and diagnosis of ASD embarked on an ambitious, 2-year program to collect samples for genetic and phenotypic research and begin analyses on these samples. The program was called The Autism Simplex Collection (TASC). TASC sample collection began in 2008 and was completed in 2010, and included nine sites from North America and four sites from Western Europe, as well as a centralized Data Coordinating Center. Over 1,700 trios are part of this collection, with DNA from transformed cells now available through the National Institute of Mental Health (NIMH). Autism Diagnostic Interview-Revised (ADI-R) and Autism Diagnostic Observation Schedule-Generic (ADOS-G) measures are available for all probands, as are standardized IQ measures, Vineland Adaptive Behavioral Scales (VABS), the Social Responsiveness Scale (SRS), Peabody Picture Vocabulary Test (PPVT), and physical measures (height, weight, and head circumference). At almost every site, additional phenotypic measures were collected, including the Broad Autism Phenotype Questionnaire (BAPQ) and Repetitive Behavior Scale-Revised (RBS-R), as well as the non-word repetition scale, Communication Checklist (Children's or Adult), and Aberrant Behavior Checklist (ABC). Moreover, for nearly 1,000 trios, the Autism Genome Project Consortium (AGP) has carried out Illumina 1 M SNP genotyping and called copy number variation (CNV) in the samples, with data being made available through the National Institutes of Health (NIH). Whole exome sequencing (WES) has been carried out in over 500 probands, together with ancestry matched controls, and this data is also available through the NIH. Additional WES is being carried out by the Autism Sequencing Consortium (ASC), where the focus is on sequencing complete trios. ASC sequencing for the first 1,000 samples (all from whole-blood DNA) is complete and data will be released in 2014. Data is being made available through NIH databases (database of Genotypes and Phenotypes (dbGaP) and National Database for Autism Research (NDAR)) with DNA released in Dist 11.0. Primary funding for the collection, genotyping, sequencing and distribution of TASC samples was provided by Autism Speaks and the NIH, including the National Institute of Mental Health (NIMH) and the National Human Genetics Research Institute (NHGRI). TASC represents an important sample set that leverages expert sites. Similar approaches, leveraging expert sites and ongoing studies, represent an important path towards further enhancing available ASD samples.

TRIO (Triplet Ionospheric Observatory) Mission

NASA Astrophysics Data System (ADS)

Lee, D.; Seon, J.; Jin, H.; Kim, K.; Lee, J.; Jang, M.; Pak, S.; Kim, K.; Lin, R. P.; Parks, G. K.; Halekas, J. S.; Larson, D. E.; Eastwood, J. P.; Roelof, E. C.; Horbury, T. S.

2009-12-01

Triplets of identical cubesats will be built to carry out the following scientific objectives: i) multi-observations of ionospheric ENA (Energetic Neutral Atom) imaging, ii) ionospheric signature of suprathermal electrons and ions associated with auroral acceleration as well as electron microbursts, and iii) complementary measurements of magnetic fields for particle data. Each satellite, a cubesat for ion, neutral, electron, and magnetic fields (CINEMA), is equipped with a suprathermal electron, ion, neutral (STEIN) instrument and a 3-axis magnetometer of magnetoresistive sensors. TRIO is developed by three institutes: i) two CINEMA by Kyung Hee University (KHU) under the WCU program, ii) one CINEMA by UC Berkeley under the NSF support, and iii) three magnetometers by Imperial College, respectively. Multi-spacecraft observations in the STEIN instruments will provide i) stereo ENA imaging with a wide angle in local times, which are sensitive to the evolution of ring current phase space distributions, ii) suprathermal electron measurements with narrow spacings, which reveal the differential signature of accelerated electrons driven by Alfven waves and/or double layer formation in the ionosphere between the acceleration region and the aurora, and iii) suprathermal ion precipitation when the storm-time ring current appears. In addition, multi-spacecraft magnetic field measurements in low earth orbits will allow the tracking of the phase fronts of ULF waves, FTEs, and quasi-periodic reconnection events between ground-based magnetometer data and upstream satellite data.

Advanced system on a chip microelectronics for spacecraft and science instruments

NASA Astrophysics Data System (ADS)

Paschalidis, Nikolaos P.

2003-01-01

The explosive growth of the modern microelectronics field opens new horizons for the development of new lightweight, low power, and smart spacecraft and science instrumentation systems in the new millennium explorations. Although this growth is mostly driven by the commercial need for low power, portable and computationally intensive products, the applicability is obvious in the space sector. The additional difficulties needed to be overcome for applicability in space include radiation hardness for total ionizing dose and single event effects (SEE), and reliability. Additionally, this new capability introduces a whole new philosophy of design and R&D, with strong implications in organizational and inter-agency program management. One key component specifically developed towards low power, small size, highly autonomous spacecraft systems, is the smart sensor remote input/output (TRIO) chip. TRIO can interface to 32 transducers with current sources/sinks and voltage sensing. It includes front-end analog signal processing, a 10-bit ADC, memory, and standard serial and parallel I/Os. These functions are very useful for spacecraft and subsystems health and status monitoring, and control actions. The key contributions of the TRIO are feasibility of modular architectures, elimination of several miles of wire harnessing, and power savings by orders of magnitude. TRIO freely operates from a single power supply 2.5- 5.5 V with power dissipation <10 mW. This system on a chip device rapidly becomes a NASA and Commercial Space standard as it is already selected by thousands in several new millennium missions, including Europa Orbiter, Mars Surveyor Program, Solar Probe, Pluto Express, Stereo, Contour, Messenger, etc. In the Science Instrumentation field common instruments that can greatly take advantage of the new technologies are: energetic-particle/plasma and wave instruments, imagers, mass spectrometers, X-ray and UV spectrographs, magnetometers, laser rangefinding instruments, etc. Common measurements that apply to many of these instruments are precise time interval measurement and high resolution read-out of solid state detectors. A precise time interval measurement chip was specially developed that achieves ˜100 ps (×10 improvement) time resolution at a power dissipation ˜20 mW (×50 improvement), dead time ˜1.5 μs (×20 improvement), and chip die size 5 mm×5 mm versus two 20 cm×20 cm doubled sided boards. This device is selected as a key enabling technology for several NASA particle, delay line imaging, and laser range finding instruments onboard (NASA Image, Messenger, etc. missions). Another device with universal application is radiation energy read-out from solid state detectors. Multi-channel low-power and end-to-end sensor input—digital output is key for the new generation instruments. The readout channel comprises of a Charge Sensitive Preamplifier with a target sensitivity of ˜1 KeV FWHM at 20 pf detector capacitance, a Shaper Amplifier with programmable time constant/gain, and an ADC. The read-out chip together with the precise time interval chip comprises the essential elements of a common particle spectroscopy instrument. To mention some more applications fast-signal acquisition—and digitization is a very useful function for a category of instrument such as mass spectroscopy and profile laser rangefinding. The single chip approach includes a high bandwidth preamplifier, fast sampling ˜5 ns, analog memory ˜10K locations, 12-bit ADC and serial/parallel I/Os. The wealth of the applications proves the advanced microelectronics field as a key enabling technology for the new millennium space exploration.

Association Between Genes Involved in Craniofacial Development and Nonsyndromic Cleft Lip and/or Palate in the Brazilian Population.

PubMed

Machado, Renato Assis; Messetti, Ana Camila; de Aquino, Sibele Nascimento; Martelli-Júnior, Hercílio; Swerts, Mário Sérgio Oliveira; de Almeida Reis, Silvia Regina; Moreira, Helenara Salvati Bertolossi; Persuhn, Darlene Camati; Coletta, Ricardo D

2016-09-01

To determine the association of single-nucleotide polymorphisms (SNPs) in genes related to craniofacial development, which were previously identified as susceptibility signals for nonsyndromic oral clefts, in Brazilians with nonsyndromic cleft lip and/or palate (NSCL/P). The SNPs rs748044 (TNP1), rs1106514 (MSX1), rs28372960, rs15251 and rs2569062 (TCOF1), rs7829058 (FGFR1), rs1793949 (COL2A1), rs11653738 (WNT3), and rs242082 (TIMP3) were assessed in a family-based transmission disequilibrium test (TDT) and a structured case-control analysis based on the individual ancestry proportions. The SNPs were initially analyzed by TDT, and polymorphisms showing a trend toward excess transmission were subsequently studied in an independent case-control sample. The study sample consisted of 189 case-parent trios of nonsyndromic cleft lip with or without cleft palate (NSCL±P), 107 case-parent trios of nonsyndromic cleft palate (NSCP), 318 isolated samples of NSCL±P, 189 isolated samples of NSCP, and 599 healthy controls. Association of alleles with NSCL/P pathogenesis. Preferential transmission of SNPs rs28372960 and rs7829058 in NSCL±P trios and rs11653738 in NSCP trios (P = .04) were observed, although the structured case-control analysis did not confirm these associations. The haplotype T-C-C formed by TCOF1 SNPs rs28372960, rs15251, and rs2569062 was more frequently transmitted from healthy parents to NSCL±P offspring, but the P value (P = .01) did not withstand Bonferroni correction for multiple tests. With the modest associations, our results do not support the hypothesis that TNP1, MSX1, TCOF1, FGFR1, COL2A1, WNT3, and TIMP3 variants are risk factors for nonsyndromic oral clefts in the Brazilian population.

Increased Frequency of De Novo Copy Number Variations in Congenital Heart Disease by Integrative Analysis of SNP Array and Exome Sequence Data

PubMed Central

Rodriguez-Murillo, Laura; Fromer, Menachem; Mazaika, Erica; Vardarajan, Badri; Italia, Michael; Leipzig, Jeremy; DePalma, Steven R.; Golhar, Ryan; Sanders, Stephan J.; Yamrom, Boris; Ronemus, Michael; Iossifov, Ivan; Willsey, A. Jeremy; State, Matthew W.; Kaltman, Jonathan R.; White, Peter S.; Shen, Yufeng; Warburton, Dorothy; Brueckner, Martina; Seidman, Christine; Goldmuntz, Elizabeth; Gelb, Bruce D.; Lifton, Richard; Seidman, Jonathan; Hakonarson, Hakon; Chung, Wendy K.

2014-01-01

Rationale Congenital heart disease (CHD) is among the most common birth defects. Most cases are of unknown etiology. Objective To determine the contribution of de novo copy number variants (CNVs) in the etiology of sporadic CHD. Methods and Results We studied 538 CHD trios using genome-wide dense single nucleotide polymorphism (SNP) arrays and/or whole exome sequencing (WES). Results were experimentally validated using digital droplet PCR. We compared validated CNVs in CHD cases to CNVs in 1,301 healthy control trios. The two complementary high-resolution technologies identified 63 validated de novo CNVs in 51 CHD cases. A significant increase in CNV burden was observed when comparing CHD trios with healthy trios, using either SNP array (p=7x10−5, Odds Ratio (OR)=4.6) or WES data (p=6x10−4, OR=3.5) and remained after removing 16% of de novo CNV loci previously reported as pathogenic (p=0.02, OR=2.7). We observed recurrent de novo CNVs on 15q11.2 encompassing CYFIP1, NIPA1, and NIPA2 and single de novo CNVs encompassing DUSP1, JUN, JUP, MED15, MED9, PTPRE SREBF1, TOP2A, and ZEB2, genes that interact with established CHD proteins NKX2-5 and GATA4. Integrating de novo variants in WES and CNV data suggests that ETS1 is the pathogenic gene altered by 11q24.2-q25 deletions in Jacobsen syndrome and that CTBP2 is the pathogenic gene in 10q sub-telomeric deletions. Conclusions We demonstrate a significantly increased frequency of rare de novo CNVs in CHD patients compared with healthy controls and suggest several novel genetic loci for CHD. PMID:25205790

Accuracy of four different digital intraoral scanners: effects of the presence of orthodontic brackets and wire.

PubMed

Jung, Yoo-Ran; Park, Ji-Man; Chun, Youn-Sic; Lee, Kkot-Nim; Kim, Minji

The objective of this study was to compare the accuracy of four different digital intraoral scanners and the effects of buccal brackets and orthodontic wire. For this study, three sets of models (Control model, BKT model with buccal bracket, and WBKT model with buccal bracket and orthodontic wire) were scanned using four different types of intraoral scanners: E4D dentist, iTero, Trios, and Zfx IntraScan. The mesiodistal width of the teeth, intercanine width, and intermolar width measured by four scanners were compared. Three-dimensional (3D) images of the brackets were taken using the four scanners. Data were analyzed with one-way ANOVA, independent t test, and post-hoc Tukey test at a significance level of P < 0.05. When comparing the 3D images with manual measurements using a traditional caliper, iTero and Trios showed the highest accuracy in horizontal measurements.iTero had the lowest values in Devmax-min of maxillary intermolar and intercanine widths (0.16 mm and 0.20 mm, respectively), whereas Trios had the lowest values in Devmax-min of mandibular intermolar and intercanine widths (0.36 mm and 0.14 mm, respectively). The horizontal variables were barely affected by the presence of buccal brackets and orthodontic wire. Comparison of 3D bracket images scanned by the four scanners showed differences in image distortion among the scanners. Bracket characteristics did not affect the 3D bracket images. The four intraoral scanners used in this study differed in accuracy. However, the results acquired by iTero and Trios were more reliable. Effects of buccal brackets and orthodontic wire on the 3D images taken by intraoral scanners were not clinically significant.

Marginal fit of all-ceramic crowns fabricated using two extraoral CAD/CAM systems in comparison with the conventional technique.

PubMed

Alqahtani, Fawaz

2017-01-01

The purpose of this study was to determine the effect of two extraoral computer-aided design (CAD) and computer-aided manufacturing (CAM) systems, in comparison with conventional techniques, on the marginal fit of monolithic CAD/CAM lithium disilicate ceramic crowns. This is an in vitro interventional study. The study was carried out at the Department of Prosthodontics, School of Dentistry, Prince Sattam Bin Abdul-Aziz University, Saudi Arabia, from December 2015 to April 2016. A marginal gap of 60 lithium disilicate crowns was evaluated by scanning electron microscopy. In total, 20 pressable lithium disilicate (IPS e.max Press [Ivoclar Vivadent]) ceramic crowns were fabricated using the conventional lost-wax technique as a control group. The experimental all-ceramic crowns were produced based on a scan stone model and milled using two extraoral CAD/CAM systems: the Cerec group was fabricated using the Cerec CAD/CAM system, and the Trios group was fabricated using Trios CAD and milled using Wieland Zenotec CAM. One-way analysis of variance (ANOVA) and the Scheffe post hoc test were used for statistical comparison of the groups (α=0.05). The mean (±standard deviation) of the marginal gap of each group was as follows: the Control group was 91.15 (±15.35) µm, the Cerec group was 111.07 (±6.33) µm, and the Trios group was 60.17 (±11.09) µm. One-way ANOVA and the Scheffe post hoc test showed a statistically significant difference in the marginal gap between all groups. It can be concluded from the current study that all-ceramic crowns, fabricated using the CAD/CAM system, show a marginal accuracy that is acceptable in clinical environments. The Trios CAD group displayed the smallest marginal gap.

Increased frequency of de novo copy number variants in congenital heart disease by integrative analysis of single nucleotide polymorphism array and exome sequence data.

PubMed

Glessner, Joseph T; Bick, Alexander G; Ito, Kaoru; Homsy, Jason; Rodriguez-Murillo, Laura; Fromer, Menachem; Mazaika, Erica; Vardarajan, Badri; Italia, Michael; Leipzig, Jeremy; DePalma, Steven R; Golhar, Ryan; Sanders, Stephan J; Yamrom, Boris; Ronemus, Michael; Iossifov, Ivan; Willsey, A Jeremy; State, Matthew W; Kaltman, Jonathan R; White, Peter S; Shen, Yufeng; Warburton, Dorothy; Brueckner, Martina; Seidman, Christine; Goldmuntz, Elizabeth; Gelb, Bruce D; Lifton, Richard; Seidman, Jonathan; Hakonarson, Hakon; Chung, Wendy K

2014-10-24

Congenital heart disease (CHD) is among the most common birth defects. Most cases are of unknown pathogenesis. To determine the contribution of de novo copy number variants (CNVs) in the pathogenesis of sporadic CHD. We studied 538 CHD trios using genome-wide dense single nucleotide polymorphism arrays and whole exome sequencing. Results were experimentally validated using digital droplet polymerase chain reaction. We compared validated CNVs in CHD cases with CNVs in 1301 healthy control trios. The 2 complementary high-resolution technologies identified 63 validated de novo CNVs in 51 CHD cases. A significant increase in CNV burden was observed when comparing CHD trios with healthy trios, using either single nucleotide polymorphism array (P=7×10(-5); odds ratio, 4.6) or whole exome sequencing data (P=6×10(-4); odds ratio, 3.5) and remained after removing 16% of de novo CNV loci previously reported as pathogenic (P=0.02; odds ratio, 2.7). We observed recurrent de novo CNVs on 15q11.2 encompassing CYFIP1, NIPA1, and NIPA2 and single de novo CNVs encompassing DUSP1, JUN, JUP, MED15, MED9, PTPRE SREBF1, TOP2A, and ZEB2, genes that interact with established CHD proteins NKX2-5 and GATA4. Integrating de novo variants in whole exome sequencing and CNV data suggests that ETS1 is the pathogenic gene altered by 11q24.2-q25 deletions in Jacobsen syndrome and that CTBP2 is the pathogenic gene in 10q subtelomeric deletions. We demonstrate a significantly increased frequency of rare de novo CNVs in CHD patients compared with healthy controls and suggest several novel genetic loci for CHD. © 2014 American Heart Association, Inc.

First depressed, then discriminated against?

PubMed

Baert, Stijn; De Visschere, Sarah; Schoors, Koen; Vandenberghe, Désirée; Omey, Eddy

2016-12-01

Each year a substantial share of the European population suffers from major depression. This mental illness may affect individuals' later life outcomes indirectly by the stigma it inflicts. The present study assesses hiring discrimination based on disclosed depression. To this end, between May 2015 and July 2015, we sent out 288 trios of job applications from fictitious candidates to real vacancies in Belgium. Within each trio, one candidate claimed to have become unemployed only recently, whereas the other two candidates revealed former depression or no reason at all for their unemployment during a full year. Disclosing a year of inactivity due to former depression decreases the probability of getting a job interview invitation by about 34% when compared with candidates who just became unemployed, but the stigma effect of a year of depression is not significantly higher than the stigma effect of a year of unexplained unemployment. In addition, we found that these stigmas of depression and unemployment were driven by our male trios of fictitious candidates. As a consequence, our results are in favour of further research on gender heterogeneity in the stigma of depression and other health impairments. Copyright © 2016 Elsevier Ltd. All rights reserved.

Two brothers' alleged paternity for a child: who is the father?

PubMed

Dogan, Muhammed; Kara, Umut; Emre, Ramazan; Fung, Wing Kam; Canturk, Kemal Murat

2015-06-01

In paternity cases where individuals are close relatives, it may be necessary to evaluate mother's DNA profile (trio test) and to increase the number of polymorphic STR loci that are analyzed. In our case, two alleged fathers who are brothers and the child (duo case) were analyzed based on 20 STR loci; however, no exclusions could be achieved. Then trio test (with mother) was performed using the Identifiler Plus kit (Applied Biosystems) and no exclusions could be achieved again. Analysis performed with the ESS Plex Plus kit (Qiagen), the paternity of one of the two alleged fathers was rejected only on 2 STR loci. We made the calculations of power of exclusion values to interpret our results more properly. The probability of exclusion (PE) is calculated as 0.9776546 in 15 loci of Identifiler Plus kit without mother. The PE is calculated as 0.9942803, if 5 additional loci from ESS Plex Plus kit are typed. The PE becomes 0.9961048 for the Identifiler Plus kit in trio analysis. If both Identifiler Plus and ESS Plex Plus kits are used for testing, the PE is calculated as 0.999431654, which indicates that the combined kits are highly discriminating.

Microgravity

NASA Image and Video Library

2000-12-15

Paul Ducheyne, a principal investigator in the microgravity materials science program and head of the University of Pernsylvania's Center for Bioactive Materials and Tissue Engineering, is leading the trio as they use simulated microgravity to determine the optimal characteristics of tiny glass particles for growing bone tissue. The result could make possible a much broader range of synthetic bone-grafting applications. Bioactive glass particles (left) with a microporous surface (right) are widely accepted as a synthetic material for periodontal procedures. Using the particles to grow three-dimensional tissue cultures may one day result in developing an improved, more rugged bone tissue that may be used to correct skeletal disorders and bone defects. The work is sponsored by NASA's Office of Biological and Physical Research.

Cadherin-11 regulates protrusive activity in Xenopus cranial neural crest cells upstream of Trio and the small GTPases.

PubMed

Kashef, Jubin; Köhler, Almut; Kuriyama, Sei; Alfandari, Dominique; Mayor, Roberto; Wedlich, Doris

2009-06-15

Xenopus Cadherin-11 (Xcad-11) is expressed when cranial neural crest cells (CNC) acquire motility. However, its function in stimulating cell migration is poorly understood. Here, we demonstrate that Xcad-11 initiates filopodia and lamellipodia formation, which is essential for CNC to populate pharyngeal pouches. We identified the cytoplasmic tail of Xcad-11 as both necessary and sufficient for proper CNC migration as long as it was linked to the plasma membrane. Our results showing that guanine nucleotide exchange factor (GEF)-Trio binds to Xcad-11 and can functionally substitute for it like constitutively active forms of RhoA, Rac, and cdc42 unravel a novel cadherin function.

Constipation and the preached trio: diet, fluid intake, exercise.

PubMed

Annells, Merilyn; Koch, Tina

2003-11-01

A survey of 90 older community-dwelling people's constipation experience is reported in part. The focus is the participants' efforts to use diet, fluid intake and exercise as preventive strategies. Most feel that they have been preached to in this regard. However, constraints may prevent full adherence to the trio and although some have gained from diet adjustment, the majority is disillusioned about these strategies. Nurses should be aware that scientific and medical literature is discussing evidence that dietary fibre intake preventing constipation is not proven, that fluid intake does not necessarily determine stool bulk or speed colon transit time, and that there is no proven link between exercise levels and chronic constipation.

The Autism Simplex Collection: an international, expertly phenotyped autism sample for genetic and phenotypic analyses

PubMed Central

2014-01-01

Background There is an urgent need for expanding and enhancing autism spectrum disorder (ASD) samples, in order to better understand causes of ASD. Methods In a unique public-private partnership, 13 sites with extensive experience in both the assessment and diagnosis of ASD embarked on an ambitious, 2-year program to collect samples for genetic and phenotypic research and begin analyses on these samples. The program was called The Autism Simplex Collection (TASC). TASC sample collection began in 2008 and was completed in 2010, and included nine sites from North America and four sites from Western Europe, as well as a centralized Data Coordinating Center. Results Over 1,700 trios are part of this collection, with DNA from transformed cells now available through the National Institute of Mental Health (NIMH). Autism Diagnostic Interview-Revised (ADI-R) and Autism Diagnostic Observation Schedule-Generic (ADOS-G) measures are available for all probands, as are standardized IQ measures, Vineland Adaptive Behavioral Scales (VABS), the Social Responsiveness Scale (SRS), Peabody Picture Vocabulary Test (PPVT), and physical measures (height, weight, and head circumference). At almost every site, additional phenotypic measures were collected, including the Broad Autism Phenotype Questionnaire (BAPQ) and Repetitive Behavior Scale-Revised (RBS-R), as well as the non-word repetition scale, Communication Checklist (Children’s or Adult), and Aberrant Behavior Checklist (ABC). Moreover, for nearly 1,000 trios, the Autism Genome Project Consortium (AGP) has carried out Illumina 1 M SNP genotyping and called copy number variation (CNV) in the samples, with data being made available through the National Institutes of Health (NIH). Whole exome sequencing (WES) has been carried out in over 500 probands, together with ancestry matched controls, and this data is also available through the NIH. Additional WES is being carried out by the Autism Sequencing Consortium (ASC), where the focus is on sequencing complete trios. ASC sequencing for the first 1,000 samples (all from whole-blood DNA) is complete and data will be released in 2014. Data is being made available through NIH databases (database of Genotypes and Phenotypes (dbGaP) and National Database for Autism Research (NDAR)) with DNA released in Dist 11.0. Primary funding for the collection, genotyping, sequencing and distribution of TASC samples was provided by Autism Speaks and the NIH, including the National Institute of Mental Health (NIMH) and the National Human Genetics Research Institute (NHGRI). Conclusions TASC represents an important sample set that leverages expert sites. Similar approaches, leveraging expert sites and ongoing studies, represent an important path towards further enhancing available ASD samples. PMID:25392729

The Great Observatories Origins Deep Survey (GOODS) Spitzer Legacy Science Program

NASA Astrophysics Data System (ADS)

Dickinson, M.; GOODS Team

2004-12-01

The Great Observatories Origins Deep Survey (GOODS) is an anthology of observing programs that are creating a rich, public, multiwavelength data set for studying galaxy formation and evolution. GOODS is observing two fields, one in each hemisphere, with extremely deep imaging and spectroscopy using the most powerful telescopes in space and on the ground. The GOODS Spitzer Legacy Science Program completes the trio of observations from NASA's Great Observatories, joining already-completed GOODS data from Chandra and Hubble. Barring unforeseen difficulties, the GOODS Spitzer observing program will have been completed by the end of 2004, and the first data products will have been released to the astronomical community. In this Special Oral Session, and in an accompanying poster session, the GOODS team presents early scientific results from this Spitzer Legacy program, as well as new research based on other GOODS data sets. I will introduce the session with a brief description of the Legacy observations and data set. Support for this work, part of the Spitzer Space Telescope Legacy Science Program, was provided by NASA through Contract Number 1224666 issued by the Jet Propulsion Laboratory, California Institute of Technology under NASA contract 1407.

The Tight-interlocked Rhythm Section: Production and Perception of Synchronisation in Jazz Trio Performance.

PubMed

Hofmann, Alex; Wesolowski, Brian C; Goebl, Werner

2017-01-01

This study investigates the production and perception of timing, synchronisation and dynamics in jazz trio performances. In a production experiment, six trio combinations of one saxophonist, two bassists, and three drummers were recorded while they performed three popular jazz songs. Onset timing and dynamics of each performer were extracted and analysed. Results showed that the tempo was significantly influenced by the timing of the drummers and all performers showed higher temporal precision on the backbeats. The drummers demonstrated individual swing-ratios, accentuations of beats and intrapersonal asynchronies between simultaneous hi-hat and ride cymbal onsets, which resulted in a hi-hat played 2-26 ms ahead of the pulse of the music. In a subsequent perception test, participants ([Formula: see text]) rated 12 excerpts of the jazz recordings. They selected their preferred version from a pool of stimuli containing the original version, but also manipulations with artificially increased or reduced asynchronies. Stimuli with reduced asynchronies smaller than 19 ms were preferred by the listeners over the original or the fully quantised timing. This suggests that listeners endorse a 'tight-interlocked' jazz rhythm section, with asynchronies smaller than the perceptual threshold (temporal masking), but with natural timing variabilities that makes it distinguishable from a computer-generated playback.

The Tight-interlocked Rhythm Section: Production and Perception of Synchronisation in Jazz Trio Performance

PubMed Central

Hofmann, Alex; Wesolowski, Brian C.; Goebl, Werner

2017-01-01

Abstract This study investigates the production and perception of timing, synchronisation and dynamics in jazz trio performances. In a production experiment, six trio combinations of one saxophonist, two bassists, and three drummers were recorded while they performed three popular jazz songs. Onset timing and dynamics of each performer were extracted and analysed. Results showed that the tempo was significantly influenced by the timing of the drummers and all performers showed higher temporal precision on the backbeats. The drummers demonstrated individual swing-ratios, accentuations of beats and intrapersonal asynchronies between simultaneous hi-hat and ride cymbal onsets, which resulted in a hi-hat played 2–26 ms ahead of the pulse of the music. In a subsequent perception test, participants () rated 12 excerpts of the jazz recordings. They selected their preferred version from a pool of stimuli containing the original version, but also manipulations with artificially increased or reduced asynchronies. Stimuli with reduced asynchronies smaller than 19 ms were preferred by the listeners over the original or the fully quantised timing. This suggests that listeners endorse a ‘tight-interlocked’ jazz rhythm section, with asynchronies smaller than the perceptual threshold (temporal masking), but with natural timing variabilities that makes it distinguishable from a computer-generated playback. PMID:29238387

Cdc42 and the Guanine Nucleotide Exchange Factors Ect2 and Trio Mediate Fn14-Induced Migration and Invasion of Glioblastoma Cells

PubMed Central

Fortin, Shannon P.; Ennis, Matthew J.; Schumacher, Cassie A.; Zylstra-Diegel, Cassandra R.; Williams, Bart O.; Ross, Julianna T.D.; Winkles, Jeffrey A.; Loftus, Joseph C.; Symons, Marc H.; Tran, Nhan L.

2012-01-01

Malignant glioblastomas are characterized by their ability to infiltrate into normal brain. We previously reported that binding of the multifunctional cytokine TNF-like weak inducer of apoptosis (TWEAK) to its receptor fibroblast growth factor–inducible 14 (Fn14) induces glioblastoma cell invasion via Rac1 activation. Here, we show that Cdc42 plays an essential role in Fn14-mediated activation of Rac1. TWEAK-treated glioma cells display an increased activation of Cdc42, and depletion of Cdc42 using siRNA abolishes TWEAK-induced Rac1 activation and abrogates glioma cell migration and invasion. In contrast, Rac1 depletion does not affect Cdc42 activation by Fn14, showing that Cdc42 mediates TWEAK-stimulated Rac1 activation. Furthermore, we identified two guanine nucleotide exchange factors (GEF), Ect2 and Trio, involved in TWEAK-induced activation of Cdc42 and Rac1, respectively. Depletion of Ect2 abrogates both TWEAK-induced Cdc42 and Rac1 activation, as well as subsequent TWEAK-Fn14–directed glioma cell migration and invasion. In contrast, Trio depletion inhibits TWEAK-induced Rac1 activation but not TWEAK-induced Cdc42 activation. Finally, inappropriate expression of Fn14 or Ect2 in mouse astrocytes in vivo using an RCAS vector system for glial-specific gene transfer in G-tva transgenic mice induces astrocyte migration within the brain, corroborating the in vitro importance of the TWEAK-Fn14 signaling cascade in glioblastoma invasion. Our results suggest that the TWEAK-Fn14 signaling axis stimulates glioma cell migration and invasion through two GEF-GTPase signaling units, Ect2-Cdc42 and Trio-Rac1. Components of the Fn14-Rho GEF-Rho GTPase signaling pathway present innovative drug targets for glioma therapy. PMID:22571869

Evaluation of the accuracy of 7 digital scanners: An in vitro analysis based on 3-dimensional comparisons.

PubMed

Renne, Walter; Ludlow, Mark; Fryml, John; Schurch, Zach; Mennito, Anthony; Kessler, Ray; Lauer, Abigail

2017-07-01

As digital impressions become more common and more digital impression systems are released onto the market, it is essential to systematically and objectively evaluate their accuracy. The purpose of this in vitro study was to evaluate and compare the trueness and precision of 6 intraoral scanners and 1 laboratory scanner in both sextant and complete-arch scenarios. Furthermore, time of scanning was evaluated and correlated with trueness and precision. A custom complete-arch model was fabricated with a refractive index similar to that of tooth structure. Seven digital impression systems were used to scan the custom model for both posterior sextant and complete arch scenarios. Analysis was performed using 3-dimensional metrology software to measure discrepancies between the master model and experimental casts. Of the intraoral scanners, the Planscan was found to have the best trueness and precision while the 3Shape Trios was found to have the poorest for sextant scanning (P<.001). The order of trueness for complete arch scanning was as follows: 3Shape D800 >iTero >3Shape TRIOS 3 >Carestream 3500 >Planscan >CEREC Omnicam >CEREC Bluecam. The order of precision for complete-arch scanning was as follows: CS3500 >iTero >3Shape D800 >3Shape TRIOS 3 >CEREC Omnicam >Planscan >CEREC Bluecam. For the secondary outcome evaluating the effect time has on trueness and precision, the complete- arch scan time was highly correlated with both trueness (r=0.771) and precision (r=0.771). For sextant scanning, the Planscan was found to be the most precise and true scanner. For complete-arch scanning, the 3Shape Trios was found to have the best balance of speed and accuracy. Copyright © 2016 Editorial Council for the Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved.

Marginal fit of all-ceramic crowns fabricated using two extraoral CAD/CAM systems in comparison with the conventional technique

PubMed Central

Alqahtani, Fawaz

2017-01-01

Objective The purpose of this study was to determine the effect of two extraoral computer-aided design (CAD) and computer-aided manufacturing (CAM) systems, in comparison with conventional techniques, on the marginal fit of monolithic CAD/CAM lithium disilicate ceramic crowns. Study design This is an in vitro interventional study. Place and duration of study The study was carried out at the Department of Prosthodontics, School of Dentistry, Prince Sattam Bin Abdul-Aziz University, Saudi Arabia, from December 2015 to April 2016. Methodology A marginal gap of 60 lithium disilicate crowns was evaluated by scanning electron microscopy. In total, 20 pressable lithium disilicate (IPS e.max Press [Ivoclar Vivadent]) ceramic crowns were fabricated using the conventional lost-wax technique as a control group. The experimental all-ceramic crowns were produced based on a scan stone model and milled using two extraoral CAD/CAM systems: the Cerec group was fabricated using the Cerec CAD/CAM system, and the Trios group was fabricated using Trios CAD and milled using Wieland Zenotec CAM. One-way analysis of variance (ANOVA) and the Scheffe post hoc test were used for statistical comparison of the groups (α=0.05). Results The mean (±standard deviation) of the marginal gap of each group was as follows: the Control group was 91.15 (±15.35) µm, the Cerec group was 111.07 (±6.33) µm, and the Trios group was 60.17 (±11.09) µm. One-way ANOVA and the Scheffe post hoc test showed a statistically significant difference in the marginal gap between all groups. Conclusion It can be concluded from the current study that all-ceramic crowns, fabricated using the CAD/CAM system, show a marginal accuracy that is acceptable in clinical environments. The Trios CAD group displayed the smallest marginal gap. PMID:28352204

Accuracy and precision of 3 intraoral scanners and accuracy of conventional impressions: A novel in vivo analysis method.

PubMed

Nedelcu, R; Olsson, P; Nyström, I; Rydén, J; Thor, A

2018-02-01

To evaluate a novel methodology using industrial scanners as a reference, and assess in vivo accuracy of 3 intraoral scanners (IOS) and conventional impressions. Further, to evaluate IOS precision in vivo. Four reference-bodies were bonded to the buccal surfaces of upper premolars and incisors in five subjects. After three reference-scans, ATOS Core 80 (ATOS), subjects were scanned three times with three IOS systems: 3M True Definition (3M), CEREC Omnicam (OMNI) and Trios 3 (TRIOS). One conventional impression (IMPR) was taken, 3M Impregum Penta Soft, and poured models were digitized with laboratory scanner 3shape D1000 (D1000). Best-fit alignment of reference-bodies and 3D Compare Analysis was performed. Precision of ATOS and D1000 was assessed for quantitative evaluation and comparison. Accuracy of IOS and IMPR were analyzed using ATOS as reference. Precision of IOS was evaluated through intra-system comparison. Precision of ATOS reference scanner (mean 0.6 μm) and D1000 (mean 0.5 μm) was high. Pairwise multiple comparisons of reference-bodies located in different tooth positions displayed a statistically significant difference of accuracy between two scanner-groups: 3M and TRIOS, over OMNI (p value range 0.0001 to 0.0006). IMPR did not show any statistically significant difference to IOS. However, deviations of IOS and IMPR were within a similar magnitude. No statistical difference was found for IOS precision. The methodology can be used for assessing accuracy of IOS and IMPR in vivo in up to five units bilaterally from midline. 3M and TRIOS had a higher accuracy than OMNI. IMPR overlapped both groups. Intraoral scanners can be used as a replacement for conventional impressions when restoring up to ten units without extended edentulous spans. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

Marginal accuracy of computer-aided design- and computer-aided manufacturing-fabricated full-arch zirconia restoration.

PubMed

Juntavee, Niwut; Sirisathit, Issarawas

2018-01-01

This study evaluated marginal accuracy of full-arch zirconia restoration fabricated from two digital computer-aided design and computer-aided manufacturing (CAD-CAM) systems (Trios-3 and CS3500) in comparison to conventional cast metal restoration. A stainless steel model comprising two canine and two molar abutments was used as a master model for full-arch reconstruction. The canine and molar abutments were machined in a cylindrical shape with 5° taper and chamfer margin. The CAD-CAM systems based on the digital approach were used to construct the full-arch zirconia restoration. The conventional cast metal restoration was fabricated according to a conventional lost-wax technique using nickel-chromium alloys. Ten restorations were fabricated from each system. The marginal accuracy of each restoration was determined at four locations for each abutment. An analysis of variance (ANOVA) and Tukey's honest significant difference (HSD) multiple comparisons were used to determine statistically significant difference at 95% confidence interval. The mean values of marginal accuracy of restorations fabricated from conventional casting, Trios-3, and CS3500 were 48.59±4.16 μm, 53.50±5.66 μm, and 56.47±5.52 μm, respectively. ANOVA indicated significant difference in marginal fit of restorations among various systems. The marginal discrepancy of zirconia restoration fabricated from the CS3500 system demonstrated significantly larger gap than that fabricated from the 3Shape system ( p <0.05). Tukey's HSD multiple comparisons indicated that the zirconia restoration fabricated from either CS3500 or Trios-3 demonstrated a significantly larger marginal gap than the conventional cast metal restoration ( p <0.05). Full-arch zirconia restoration fabricated from the Trios-3 illustrated better marginal fits than that from the CS3500, although, both were slightly less accurate than the conventional cast restoration. However, the marginal discrepancies of restoration produced by both CAD-CAM systems were within the clinically acceptable range and satisfactorily precise to be suggested for construction full-arch zirconia restoration.

30 CFR 900.13 - Federal programs and Federal coal exploration programs.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Federal programs and Federal coal exploration... INTRODUCTION § 900.13 Federal programs and Federal coal exploration programs. The rules for each Federal program and Federal coal exploration program are codified below under the assigned part for the particular...

30 CFR 900.13 - Federal programs and Federal coal exploration programs.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Federal programs and Federal coal exploration... INTRODUCTION § 900.13 Federal programs and Federal coal exploration programs. The rules for each Federal program and Federal coal exploration program are codified below under the assigned part for the particular...

30 CFR 900.13 - Federal programs and Federal coal exploration programs.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Federal programs and Federal coal exploration... INTRODUCTION § 900.13 Federal programs and Federal coal exploration programs. The rules for each Federal program and Federal coal exploration program are codified below under the assigned part for the particular...

30 CFR 900.13 - Federal programs and Federal coal exploration programs.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Federal programs and Federal coal exploration... INTRODUCTION § 900.13 Federal programs and Federal coal exploration programs. The rules for each Federal program and Federal coal exploration program are codified below under the assigned part for the particular...

30 CFR 900.13 - Federal programs and Federal coal exploration programs.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Federal programs and Federal coal exploration... INTRODUCTION § 900.13 Federal programs and Federal coal exploration programs. The rules for each Federal program and Federal coal exploration program are codified below under the assigned part for the particular...

Cadherin-11 regulates protrusive activity in Xenopus cranial neural crest cells upstream of Trio and the small GTPases

PubMed Central

Kashef, Jubin; Köhler, Almut; Kuriyama, Sei; Alfandari, Dominique; Mayor, Roberto; Wedlich, Doris

2009-01-01

Xenopus Cadherin-11 (Xcad-11) is expressed when cranial neural crest cells (CNC) acquire motility. However, its function in stimulating cell migration is poorly understood. Here, we demonstrate that Xcad-11 initiates filopodia and lamellipodia formation, which is essential for CNC to populate pharyngeal pouches. We identified the cytoplasmic tail of Xcad-11 as both necessary and sufficient for proper CNC migration as long as it was linked to the plasma membrane. Our results showing that guanine nucleotide exchange factor (GEF)-Trio binds to Xcad-11 and can functionally substitute for it like constitutively active forms of RhoA, Rac, and cdc42 unravel a novel cadherin function. PMID:19528317

Bare Bones of Bioactive Glass

NASA Technical Reports Server (NTRS)

2000-01-01

Paul Ducheyne, a principal investigator in the microgravity materials science program and head of the University of Pernsylvania's Center for Bioactive Materials and Tissue Engineering, is leading the trio as they use simulated microgravity to determine the optimal characteristics of tiny glass particles for growing bone tissue. The result could make possible a much broader range of synthetic bone-grafting applications. Bioactive glass particles (left) with a microporous surface (right) are widely accepted as a synthetic material for periodontal procedures. Using the particles to grow three-dimensional tissue cultures may one day result in developing an improved, more rugged bone tissue that may be used to correct skeletal disorders and bone defects. The work is sponsored by NASA's Office of Biological and Physical Research.

PRKCA: A Positional Candidate Gene for Body Mass Index and Asthma

PubMed Central

Murphy, Amy; Tantisira, Kelan G.; Soto-Quirós, Manuel E.; Avila, Lydiana; Klanderman, Barbara J.; Lake, Stephen; Weiss, Scott T.; Celedón, Juan C.

2009-01-01

Asthma incidence and prevalence are higher in obese individuals. A potential mechanistic basis for this relationship is pleiotropy. We hypothesized that significant linkage and candidate-gene association would be found for body mass index (BMI) in a population ascertained on asthma affection status. Linkage analysis for BMI was performed on 657 subjects in eight Costa Rican families enrolled in a study of asthma. Family-based association studies were conducted for BMI with SNPs within a positional candidate gene, PRKCA. SNPs within PRKCA were also tested for association with asthma. Association studies were conducted in 415 Costa Rican parent-child trios and 493 trios participating in the Childhood Asthma Management Program (CAMP). Although only modest evidence of linkage for BMI was obtained for the whole cohort, significant linkage was noted for BMI in females on chromosome 17q (peak LOD = 3.39). Four SNPs in a candidate gene in this region (PRKCA) had unadjusted association p values < 0.05 for BMI in both cohorts, with the joint p value for two SNPs remaining significant after adjustment for multiple comparisons (rs228883 and rs1005651, joint p values = 9.5 × 10−5 and 5.6 × 10−5). Similarly, eight SNPs had unadjusted association p values < 0.05 for asthma in both populations, with one SNP remaining significant after adjustment for multiple comparisons (rs11079657, joint p value = 2.6 × 10−5). PRKCA is a pleiotropic locus that is associated with both BMI and asthma and that has been identified via linkage analysis of BMI in a population ascertained on asthma. PMID:19576566

iReport: a generalised Galaxy solution for integrated experimental reporting.

PubMed

Hiltemann, Saskia; Hoogstrate, Youri; der Spek, Peter van; Jenster, Guido; Stubbs, Andrew

2014-01-01

Galaxy offers a number of visualisation options with components, such as Trackster, Circster and Galaxy Charts, but currently lacks the ability to easily combine outputs from different tools into a single view or report. A number of tools produce HTML reports as output in order to combine the various output files from a single tool; however, this requires programming and knowledge of HTML, and the reports must be custom-made for each new tool. We have developed a generic and flexible reporting tool for Galaxy, iReport, that allows users to create interactive HTML reports directly from the Galaxy UI, with the ability to combine an arbitrary number of outputs from any number of different tools. Content can be organised into different tabs, and interactivity can be added to components. To demonstrate the capability of iReport we provide two publically available examples, the first is an iReport explaining about iReports, created for, and using content from the recent Galaxy Community Conference 2014. The second is a genetic report based on a trio analysis to determine candidate pathogenic variants which uses our previously developed Galaxy toolset for whole-genome NGS analysis, CGtag. These reports may be adapted for outputs from any sequencing platform and any results, such as omics data, non-high throughput results and clinical variables. iReport provides a secure, collaborative, and flexible web-based reporting system that is compatible with Galaxy (and non-Galaxy) generated content. We demonstrate its value with a real-life example of reporting genetic trio-analysis.

Infestation and seasonal activity of Ixodes vespertilionis Koch, 1844 (Acari: Ixodidae) on the Maghreb mouse-eared bat, Myotis punicus Felten, 1977, in northeastern Algeria.

PubMed

Bendjeddou, Mohammed Lamine; Bouslama, Zihad; Amr, Zuhair S; BaniHani, Rihan

2016-06-01

Infestation of Ixodes vespertilionis Koch, 1844 on Myotis punicus Felten, 1977 from two sites (Trios Tunnel and Sidi Trad cave) in northeastern Algeria was studied. An overall infestation of 41.4% for all stages was found among bats collected from both sites. By stage, a total of eight females, 70 nymphs, and 107 larvae were recovered from both populations. The number of females recovered per bat at Sidi Trad ranged from 0-1, for nymphs 0-2, and for larvae 0-2. While no female ticks were collected at Trios Tunnel, the number of nymphs ranged from 0-2 and for larvae 0-2. At Trios Tunnel, the number of nymphs was significantly higher during April and June but not for July and September. On the other hand, the number of larvae increased from July to November, while at Sidi Trad cave, female ticks were recovered during April and May and then disappeared until the end of the study period. Significant differences were noted during all the months when compared with all stages. Nymphs infested bats significantly during April and May, declined in June and July, and then became steady until October. Larvae peaked in July, with low frequency in April, and then fluctuated from August to November. © 2016 The Society for Vector Ecology.

[Genetic polymorphisms of 21 non-CODIS STR loci].

PubMed

Shao, Wei-bo; Zhang, Su-hua; Li, Li

2011-02-01

To investigate genetic polymorphisms of 21 non-CODIS STR loci in Han population from the east of China and to explore their forensic application value. Twenty-one non-CODIS STR loci, were amplified with AGCU 21+1 STR kit and DNA samples were obtained from 225 unrelated individuals of the Han population from the east of China. The PCR products were analyzed with 3130 Genetic Analyzer and genotyped with GeneMapper ID v3.2 software. The genetic data were statistically analyzed with PowerStats v12.xls and Cervus 2.0 software. The distributions of 21 non-CODIS STR loci satisfied the Hardy-Weinberg equilibration. The heterozygosity (H) distributions were 0.596-0.804, the discrimination power (DP) were 0.764-0.948, the probability of exclusion of duo-testing (PEduo) were 0.176-0.492, the probability of exclusion of trios-testing (PEtrio) were 0.334-0.663, and the polymorphic information content (PIC) were 0.522-0.807. The cumulative probability of exclusion (CPE) of duo-testing was 0.999707, the CPE of trios-testing was 0.9999994, and the cumulated discrimination power (CDP) was 0.99999999999999999994. Twenty-one non-CODIS STR loci are highly polymorphic. They can be effectively used in personal identification and paternity testing in trios cases. They can also be used as supplement in the difficult cases of diad paternity testing.

The Physics Learning Program at UW-Madison: Strategies for Creating an Inclusive Learning Environment

NASA Astrophysics Data System (ADS)

Nossal, S. M.; Watson, L. E.; Jacob, A. T.; Reading, J. A.

2005-05-01

The Physics Learning Program at the University of Wisconsin-Madison provides a supportive learning environment for introductory physics students potentially at-risk for having academic trouble or for feeling isolated at the University. Physics is a gateway course for many undergraduate science majors such as biology, physics, geophysics, atmospheric science, and astronomy, and for pre-health professions. Many students struggle with their physics courses due to factors including large class sizes, isolation and lack of study partners, and/or lack of confidence in mathematical problem solving skills. Our students include those with learning disabilities, no high school physics, weak math backgrounds, and/or on academic probation. We also work with students who may be feeling isolated, such as students from historically underrepresented racial and ethnic groups, first generation college students, returning adults, international students, and students from small rural schools. Many of our students are also part of retention programs such as the TRIO program, the Academic Advancement Program, the McNair Scholars Program, and the McBurney Disability Resource Center. The Physics Learning Program's Peer Mentor Tutor program is run in conjunction with similar programs for chemistry and biochemistry at the University of Wisconsin. We will discuss strategies we use for creating an inclusive learning environment that engages students in their learning. Such strategies include small group instruction, ongoing training of the tutors, teaching problem solving skills, and creating a welcoming atmosphere.

Judging adaptive management practices of U.S. agencies.

PubMed

Fischman, Robert L; Ruhl, J B

2016-04-01

All U.S. federal agencies administering environmental laws purport to practice adaptive management (AM), but little is known about how they actually implement this conservation tool. A gap between the theory and practice of AM is revealed in judicial decisions reviewing agency adaptive management plans. We analyzed all U.S. federal court opinions published through 1 January 2015 to identify the agency AM practices courts found most deficient. The shortcomings included lack of clear objectives and processes, monitoring thresholds, and defined actions triggered by thresholds. This trio of agency shortcuts around critical, iterative steps characterizes what we call AM-lite. Passive AM differs from active AM in its relative lack of management interventions through experimental strategies. In contrast, AM-lite is a distinctive form of passive AM that fails to provide for the iterative steps necessary to learn from management. Courts have developed a sophisticated understanding of AM and often offer instructive rather than merely critical opinions. The role of the judiciary is limited by agency discretion under U.S. administrative law. But courts have overturned some agency AM-lite practices and insisted on more rigorous analyses to ensure that the promised benefits of structured learning and fine-tuned management have a reasonable likelihood of occurring. Nonetheless, there remains a mismatch in U.S. administrative law between the flexibility demanded by adaptive management and the legal objectives of transparency, public participation, and finality. © 2015 Society for Conservation Biology.

A Trio of Brownian Donkeys

NASA Astrophysics Data System (ADS)

van den Broeck, C.; Cleuren, B.; Kawai, R.; Kambon, M.

A previously introduced model (B. Cleuren and C. Van den Broeck, Europhys. Lett. 54, 1 (2001)) is studied numerically. Pure negative mobility is found for the minimum number of three interacting walkers.

41 CFR 101-29.221 - Federal Specifications, Standards and Commercial Item Description Program (Federal...

Code of Federal Regulations, 2010 CFR

2010-07-01

... standardization program developed under authority of the Federal Property and Administrative Services Act of 1949..., Standards and Commercial Item Description Program (Federal Standardization Program). 101-29.221 Section 101...-Definitions § 101-29.221 Federal Specifications, Standards and Commercial Item Description Program (Federal...

41 CFR 101-29.221 - Federal Specifications, Standards and Commercial Item Description Program (Federal...

Code of Federal Regulations, 2011 CFR

2011-07-01

... standardization program developed under authority of the Federal Property and Administrative Services Act of 1949..., Standards and Commercial Item Description Program (Federal Standardization Program). 101-29.221 Section 101...-Definitions § 101-29.221 Federal Specifications, Standards and Commercial Item Description Program (Federal...

Marginal accuracy of computer-aided design- and computer-aided manufacturing-fabricated full-arch zirconia restoration

PubMed Central

Sirisathit, Issarawas

2018-01-01

Objective This study evaluated marginal accuracy of full-arch zirconia restoration fabricated from two digital computer-aided design and computer-aided manufacturing (CAD-CAM) systems (Trios-3 and CS3500) in comparison to conventional cast metal restoration. Materials and methods A stainless steel model comprising two canine and two molar abutments was used as a master model for full-arch reconstruction. The canine and molar abutments were machined in a cylindrical shape with 5° taper and chamfer margin. The CAD-CAM systems based on the digital approach were used to construct the full-arch zirconia restoration. The conventional cast metal restoration was fabricated according to a conventional lost-wax technique using nickel–chromium alloys. Ten restorations were fabricated from each system. The marginal accuracy of each restoration was determined at four locations for each abutment. An analysis of variance (ANOVA) and Tukey’s honest significant difference (HSD) multiple comparisons were used to determine statistically significant difference at 95% confidence interval. Results The mean values of marginal accuracy of restorations fabricated from conventional casting, Trios-3, and CS3500 were 48.59±4.16 μm, 53.50±5.66 μm, and 56.47±5.52 μm, respectively. ANOVA indicated significant difference in marginal fit of restorations among various systems. The marginal discrepancy of zirconia restoration fabricated from the CS3500 system demonstrated significantly larger gap than that fabricated from the 3Shape system (p<0.05). Tukey’s HSD multiple comparisons indicated that the zirconia restoration fabricated from either CS3500 or Trios-3 demonstrated a significantly larger marginal gap than the conventional cast metal restoration (p<0.05). Conclusion Full-arch zirconia restoration fabricated from the Trios-3 illustrated better marginal fits than that from the CS3500, although, both were slightly less accurate than the conventional cast restoration. However, the marginal discrepancies of restoration produced by both CAD-CAM systems were within the clinically acceptable range and satisfactorily precise to be suggested for construction full-arch zirconia restoration. PMID:29497334

Control Requirements to Support Manual Piloting Capability

NASA Technical Reports Server (NTRS)

Merancy, Nujoud; Chevray, Kay; Gonzalez, Rodolfo; Madsen, Jennifer; Spehar, Pete

2013-01-01

The manual piloting requirements specified under the NASA Constellation Program involved Cooper-Harper ratings, which are a qualitative and subjective evaluation from experienced pilots. This type of verification entails a significant investment of resources to assess a completed design and is not one that can easily or meaningfully be applied upfront in the design phase. The evolution of the Multi-Purpose Crew Vehicle Program to include an independently developed propulsion system from an international partner makes application of Cooper-Harper based design requirements inadequate. To mitigate this issue, a novel solution was developed to reformulate the necessary piloting capability into quantifiable requirements. A trio of requirements was designed which specify control authority, precision, and impulse residuals enabling propulsion design within specified guidance and control boundaries. These requirements have been evaluated against both the existing Orion design and the proposed ESA design and have been found to achieve the desired specificity. The requirement set is capable of being applied to the development of other spacecraft in support of manual piloting.

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2012 CFR

2012-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2013 CFR

2013-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2014 CFR

2014-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2010 CFR

2010-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

31 CFR 205.16 - What special rules apply to Federal assistance programs and projects funded by the Federal...

Code of Federal Regulations, 2011 CFR

2011-07-01

... assistance programs and projects funded by the Federal Highway Trust Fund? 205.16 Section 205.16 Money and... special rules apply to Federal assistance programs and projects funded by the Federal Highway Trust Fund? The following applies to Federal assistance programs and projects funded out of the Federal Highway...

Of graphs and giggles

NASA Astrophysics Data System (ADS)

Commissariat, Tushna

2018-02-01

In recent years, there seems to have been a boom in science stand-up and theatre. The geeky comedy trio who make Festival of the Spoken Nerd deliver shows that are funny, clever and sometimes downright silly.

NASA GRACE Sees a Drying California

NASA Image and Video Library

2014-10-01

This trio of images depicts satellite observations of declining water storage in California as seen by NASA Gravity Recovery and Climate Experiment satellites in June 2002 left, June 2008 center and June 2014 right.

MTHFR c.677C>T is a risk factor for non-syndromic cleft lip with or without cleft palate in Chile.

PubMed

Ramírez-Chau, C; Blanco, R; Colombo, A; Pardo, R; Suazo, J

2016-10-01

The functional variant within the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene c.677C>T, producing alterations in folate metabolism, has been associated with the risk of non-syndromic cleft lip with or without cleft palate (NSCL/P). We assessed this association in a Chilean population using a combined analysis of case-control and case-parent trio samples. Samples of 165 cases and 291 controls and 121 case-parent trios (sharing the cases) were genotyped. Odds ratio (OR) was estimated for case-control (allele and genotype frequency differences), and this result was confirmed by allele transmission distortion in trios. Due to that these samples are not independent, a combined OR was also computed. Maternal genotype effect was additionally evaluated based on a log-linear method. Borderline but not significant OR (1.28; CI 0.97-1.69) was observed for risk allele (T) in the case-control sample. However, triad sample showed a significant association (OR 1.56: CI 1.09-2.25) which was confirmed by the combined OR (1.37; CI 1.11-1.71). Maternal genotype has been also associated with the phenotype (P = 0.002). In contrast to previous reports considering Chilean subjects, our results demonstrated that the offspring and maternal genotypes for MTHFR c.677C>T variant are strongly associated with NSCL/P in this Chilean population. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

30 CFR 736.22 - Contents of a Federal program.

Code of Federal Regulations, 2012 CFR

2012-07-01

... INTERIOR PERMANENT REGULATORY PROGRAMS FOR NON-FEDERAL AND NON-INDIAN LANDS FEDERAL PROGRAM FOR A STATE § 736.22 Contents of a Federal program. (a) In promulgating or revising any Federal program for a State, the Director shall— (1) Consider the nature of that State's soils, topography, climate, and biological...

30 CFR 736.22 - Contents of a Federal program.

Code of Federal Regulations, 2014 CFR

2014-07-01

... INTERIOR PERMANENT REGULATORY PROGRAMS FOR NON-FEDERAL AND NON-INDIAN LANDS FEDERAL PROGRAM FOR A STATE § 736.22 Contents of a Federal program. (a) In promulgating or revising any Federal program for a State, the Director shall— (1) Consider the nature of that State's soils, topography, climate, and biological...

30 CFR 736.22 - Contents of a Federal program.

Code of Federal Regulations, 2011 CFR

2011-07-01

... INTERIOR PERMANENT REGULATORY PROGRAMS FOR NON-FEDERAL AND NON-INDIAN LANDS FEDERAL PROGRAM FOR A STATE § 736.22 Contents of a Federal program. (a) In promulgating or revising any Federal program for a State, the Director shall— (1) Consider the nature of that State's soils, topography, climate, and biological...

30 CFR 736.22 - Contents of a Federal program.

Code of Federal Regulations, 2013 CFR

2013-07-01

... INTERIOR PERMANENT REGULATORY PROGRAMS FOR NON-FEDERAL AND NON-INDIAN LANDS FEDERAL PROGRAM FOR A STATE § 736.22 Contents of a Federal program. (a) In promulgating or revising any Federal program for a State, the Director shall— (1) Consider the nature of that State's soils, topography, climate, and biological...

45 CFR 2523.100 - Are participants in programs operated by Federal agencies Federal employees?

Code of Federal Regulations, 2010 CFR

2010-10-01

... PROVISION OF AMERICORPS PROGRAM ASSISTANCE § 2523.100 Are participants in programs operated by Federal agencies Federal employees? No. Participants in these programs have the same employee status as participants in other approved AmeriCorps programs, and are not considered Federal employees, except for the...

2 CFR 200.519 - Criteria for Federal program risk.

Code of Federal Regulations, 2014 CFR

2014-01-01

... program in its life cycle at the Federal agency may indicate risk. For example, a new Federal program with... of Federal awards may increase risk. (3) The phase of a Federal program in its life cycle at the...

Haplotype Phasing and Inheritance of Copy Number Variants in Nuclear Families

PubMed Central

Palta, Priit; Kaplinski, Lauris; Nagirnaja, Liina; Veidenberg, Andres; Möls, Märt; Nelis, Mari; Esko, Tõnu; Metspalu, Andres; Laan, Maris; Remm, Maido

2015-01-01

DNA copy number variants (CNVs) that alter the copy number of a particular DNA segment in the genome play an important role in human phenotypic variability and disease susceptibility. A number of CNVs overlapping with genes have been shown to confer risk to a variety of human diseases thus highlighting the relevance of addressing the variability of CNVs at a higher resolution. So far, it has not been possible to deterministically infer the allelic composition of different haplotypes present within the CNV regions. We have developed a novel computational method, called PiCNV, which enables to resolve the haplotype sequence composition within CNV regions in nuclear families based on SNP genotyping microarray data. The algorithm allows to i) phase normal and CNV-carrying haplotypes in the copy number variable regions, ii) resolve the allelic copies of rearranged DNA sequence within the haplotypes and iii) infer the heritability of identified haplotypes in trios or larger nuclear families. To our knowledge this is the first program available that can deterministically phase null, mono-, di-, tri- and tetraploid genotypes in CNV loci. We applied our method to study the composition and inheritance of haplotypes in CNV regions of 30 HapMap Yoruban trios and 34 Estonian families. For 93.6% of the CNV loci, PiCNV enabled to unambiguously phase normal and CNV-carrying haplotypes and follow their transmission in the corresponding families. Furthermore, allelic composition analysis identified the co-occurrence of alternative allelic copies within 66.7% of haplotypes carrying copy number gains. We also observed less frequent transmission of CNV-carrying haplotypes from parents to children compared to normal haplotypes and identified an emergence of several de novo deletions and duplications in the offspring. PMID:25853576

Haplotype phasing and inheritance of copy number variants in nuclear families.

PubMed

Palta, Priit; Kaplinski, Lauris; Nagirnaja, Liina; Veidenberg, Andres; Möls, Märt; Nelis, Mari; Esko, Tõnu; Metspalu, Andres; Laan, Maris; Remm, Maido

2015-01-01

DNA copy number variants (CNVs) that alter the copy number of a particular DNA segment in the genome play an important role in human phenotypic variability and disease susceptibility. A number of CNVs overlapping with genes have been shown to confer risk to a variety of human diseases thus highlighting the relevance of addressing the variability of CNVs at a higher resolution. So far, it has not been possible to deterministically infer the allelic composition of different haplotypes present within the CNV regions. We have developed a novel computational method, called PiCNV, which enables to resolve the haplotype sequence composition within CNV regions in nuclear families based on SNP genotyping microarray data. The algorithm allows to i) phase normal and CNV-carrying haplotypes in the copy number variable regions, ii) resolve the allelic copies of rearranged DNA sequence within the haplotypes and iii) infer the heritability of identified haplotypes in trios or larger nuclear families. To our knowledge this is the first program available that can deterministically phase null, mono-, di-, tri- and tetraploid genotypes in CNV loci. We applied our method to study the composition and inheritance of haplotypes in CNV regions of 30 HapMap Yoruban trios and 34 Estonian families. For 93.6% of the CNV loci, PiCNV enabled to unambiguously phase normal and CNV-carrying haplotypes and follow their transmission in the corresponding families. Furthermore, allelic composition analysis identified the co-occurrence of alternative allelic copies within 66.7% of haplotypes carrying copy number gains. We also observed less frequent transmission of CNV-carrying haplotypes from parents to children compared to normal haplotypes and identified an emergence of several de novo deletions and duplications in the offspring.

31 CFR 205.1 - What Federal assistance programs are covered by this part?

Code of Federal Regulations, 2013 CFR

2013-07-01

...; and (2) All Federal program agencies, except the Tennessee Valley Authority (TVA) and its Federal assistance programs. (b) Only programs listed in the Catalog of Federal Domestic Assistance, as established... 31 Money and Finance:Treasury 2 2013-07-01 2013-07-01 false What Federal assistance programs are...

31 CFR 205.1 - What Federal assistance programs are covered by this part?

Code of Federal Regulations, 2011 CFR

2011-07-01

...; and (2) All Federal program agencies, except the Tennessee Valley Authority (TVA) and its Federal assistance programs. (b) Only programs listed in the Catalog of Federal Domestic Assistance, as established... 31 Money and Finance:Treasury 2 2011-07-01 2011-07-01 false What Federal assistance programs are...

31 CFR 205.1 - What Federal assistance programs are covered by this part?

Code of Federal Regulations, 2014 CFR

2014-07-01

...; and (2) All Federal program agencies, except the Tennessee Valley Authority (TVA) and its Federal assistance programs. (b) Only programs listed in the Catalog of Federal Domestic Assistance, as established... 31 Money and Finance: Treasury 2 2014-07-01 2014-07-01 false What Federal assistance programs are...

31 CFR 205.1 - What Federal assistance programs are covered by this part?

Code of Federal Regulations, 2012 CFR

2012-07-01

...; and (2) All Federal program agencies, except the Tennessee Valley Authority (TVA) and its Federal assistance programs. (b) Only programs listed in the Catalog of Federal Domestic Assistance, as established... 31 Money and Finance:Treasury 2 2012-07-01 2012-07-01 false What Federal assistance programs are...

31 CFR 205.5 - What are the thresholds for major Federal assistance programs?

Code of Federal Regulations, 2010 CFR

2010-07-01

... assistance programs? (a) Table A of this section defines major Federal assistance programs based on the... locate the appropriate row in Column A based upon the total amount of Federal assistance received. In... Federal Assistance Program means any Federal assistance program that exceed these levels: Between zero and...

A Trio of Brown Dwarfs Artist Concept

NASA Image and Video Library

2011-08-23

This artist conception based on data from NASA Wide-field Infrared Survey Explorer illustrates what brown dwarfs of different types might look like to a hypothetical interstellar traveler who has flown a spaceship to each one.

31 CFR 205.3 - What Federal assistance programs are subject to this subpart A?

Code of Federal Regulations, 2011 CFR

2011-07-01

... programs which: (1) Are listed in the Catalog of Federal Domestic Assistance; (2) Meet the funding... Federal assistance programs subject to subpart A if a State or Federal Program Agency fails to comply with... 31 Money and Finance:Treasury 2 2011-07-01 2011-07-01 false What Federal assistance programs are...

31 CFR 205.3 - What Federal assistance programs are subject to this subpart A?

Code of Federal Regulations, 2012 CFR

2012-07-01

... programs which: (1) Are listed in the Catalog of Federal Domestic Assistance; (2) Meet the funding... Federal assistance programs subject to subpart A if a State or Federal Program Agency fails to comply with... 31 Money and Finance:Treasury 2 2012-07-01 2012-07-01 false What Federal assistance programs are...

31 CFR 205.3 - What Federal assistance programs are subject to this subpart A?

Code of Federal Regulations, 2013 CFR

2013-07-01

... programs which: (1) Are listed in the Catalog of Federal Domestic Assistance; (2) Meet the funding... Federal assistance programs subject to subpart A if a State or Federal Program Agency fails to comply with... 31 Money and Finance:Treasury 2 2013-07-01 2013-07-01 false What Federal assistance programs are...

31 CFR 205.3 - What Federal assistance programs are subject to this subpart A?

Code of Federal Regulations, 2014 CFR

2014-07-01

... programs which: (1) Are listed in the Catalog of Federal Domestic Assistance; (2) Meet the funding... Federal assistance programs subject to subpart A if a State or Federal Program Agency fails to comply with... 31 Money and Finance: Treasury 2 2014-07-01 2014-07-01 false What Federal assistance programs are...

31 CFR 205.3 - What Federal assistance programs are subject to this subpart A?

Code of Federal Regulations, 2010 CFR

2010-07-01

... programs which: (1) Are listed in the Catalog of Federal Domestic Assistance; (2) Meet the funding... Federal assistance programs subject to subpart A if a State or Federal Program Agency fails to comply with... 31 Money and Finance: Treasury 2 2010-07-01 2010-07-01 false What Federal assistance programs are...

7 CFR 3052.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2011 CFR

2011-01-01

... program in its life cycle at the Federal agency may indicate risk. For example, a new Federal program with... or grant agreements may increase risk. (3) The phase of a Federal program in its life cycle at the... part of the risk analysis, the auditor may wish to discuss a particular Federal program with auditee...

7 CFR 3052.520 - Major program determination.

Code of Federal Regulations, 2011 CFR

2011-01-01

... shall be labeled Type A programs. Type A programs are defined as Federal programs with Federal awards... Federal awards expended exceed $10 billion. (2) Federal programs not labeled Type A under paragraph (b)(1) of this section shall be labeled Type B programs. (3) The inclusion of large loan and loan guarantees...

49 CFR 613.300 - Coordination of Federal and federally assisted programs and projects.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Coordination of Federal and federally assisted programs and projects. 613.300 Section 613.300 Transportation Other Regulations Relating to Transportation... Coordination of Federal and Federally Assisted Programs and Projects § 613.300 Coordination of Federal and...

76 FR 5787 - Federal Perkins Loan, Federal Work-Study, and Federal Supplemental Educational Opportunity Grant...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-02

... DEPARTMENT OF EDUCATION [CFDA Nos. 84.038, 84.033, and 84.007] Federal Perkins Loan, Federal Work... for the campus-based programs. SUPPLEMENTARY INFORMATION: The Federal Perkins Loan, Federal Work-Study.... The Work Colleges Program The Work Colleges September 30, Report of 2010-2011 award Program Report can...

2 CFR 200.202 - Requirement to provide public notice of Federal financial assistance programs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... of Federal financial assistance programs. (a) The Federal awarding agency must notify the public of Federal programs in the Catalog of Federal Domestic Assistance (CFDA), maintained by the General Services... branch of the Federal government. (2) The information that the Federal awarding agency must submit to GSA...

Hippo-independent activation of YAP by the GNAQ uveal melanoma oncogene through a trio-regulated rho GTPase signaling circuitry.

PubMed

Feng, Xiaodong; Degese, Maria Sol; Iglesias-Bartolome, Ramiro; Vaque, Jose P; Molinolo, Alfredo A; Rodrigues, Murilo; Zaidi, M Raza; Ksander, Bruce R; Merlino, Glenn; Sodhi, Akrit; Chen, Qianming; Gutkind, J Silvio

2014-06-16

Mutually exclusive activating mutations in the GNAQ and GNA11 oncogenes, encoding heterotrimeric Gαq family members, have been identified in ∼ 83% and ∼ 6% of uveal and skin melanomas, respectively. However, the molecular events underlying these GNAQ-driven malignancies are not yet defined, thus limiting the ability to develop cancer-targeted therapies. Here, we focused on the transcriptional coactivator YAP, a critical component of the Hippo signaling pathway that controls organ size. We found that Gαq stimulates YAP through a Trio-Rho/Rac signaling circuitry promoting actin polymerization, independently of phospholipase Cβ and the canonical Hippo pathway. Furthermore, we show that Gαq promotes the YAP-dependent growth of uveal melanoma cells, thereby identifying YAP as a suitable therapeutic target in uveal melanoma, a GNAQ/GNA11-initiated human malignancy. Copyright © 2014 Elsevier Inc. All rights reserved.

Variation in genome-wide mutation rates within and between human families.

PubMed

Conrad, Donald F; Keebler, Jonathan E M; DePristo, Mark A; Lindsay, Sarah J; Zhang, Yujun; Casals, Ferran; Idaghdour, Youssef; Hartl, Chris L; Torroja, Carlos; Garimella, Kiran V; Zilversmit, Martine; Cartwright, Reed; Rouleau, Guy A; Daly, Mark; Stone, Eric A; Hurles, Matthew E; Awadalla, Philip

2011-06-12

J.B.S. Haldane proposed in 1947 that the male germline may be more mutagenic than the female germline. Diverse studies have supported Haldane's contention of a higher average mutation rate in the male germline in a variety of mammals, including humans. Here we present, to our knowledge, the first direct comparative analysis of male and female germline mutation rates from the complete genome sequences of two parent-offspring trios. Through extensive validation, we identified 49 and 35 germline de novo mutations (DNMs) in two trio offspring, as well as 1,586 non-germline DNMs arising either somatically or in the cell lines from which the DNA was derived. Most strikingly, in one family, we observed that 92% of germline DNMs were from the paternal germline, whereas, in contrast, in the other family, 64% of DNMs were from the maternal germline. These observations suggest considerable variation in mutation rates within and between families.

Appraisal of Bleb Using Trio of Intraocular Pressure, Morphology on Slit Lamp, and Gonioscopy.

PubMed

Thatte, Shreya; Rana, Rimpi; Gaur, Neeraj

2016-01-01

The aim of this study was to assess bleb function using Wuerzburg bleb classification score (WBCS) for bleb morphology on slit lamp, intraocular pressure (IOP), and gonioscopy. A total of randomly selected 30 eyes posttrabeculectomy were assessed for bleb function with the trio of bleb morphology, IOP, and gonioscopy. Bleb was assessed using the WBCS of 0-12 on slit lamp, IOP was assessed using applanation tonometry, and visualization of inner ostium and iridectomy were assessed using gonioscopy. Postoperative patients of less than six weeks were excluded from the study. The correlation between WBCS and the duration of trabeculectomy was found to be highly significant ( P value = 0.029). The correlation of IOP with WBCS was also found to be strongly positive ( P = 0.000). IOP was found to be highly associated with peripheral iridectomy ( P = 0.000), internal window ( P = 0.001), and bleb characteristics.

ISS Expedition 48-49 prime crewmembers Kate Rubins of NASA (left), Anatoly Ivanishin of Roscosmos (center) and Takuya Onishi of the Japan Aerospace Exploration Agency (right) pose for pictures with schoolchildren after arriving in Baikonur, Kazakhstan June 24 for final pre-launch training following a flight from Star City, Russia. The trio will launch July 7 from the Baikonur Cosmodrome in Kazakhstan on the Soyuz MS-01 spacecraft for a planned four-month mission on the International Space Station...NASA/Alexander Vysotsky.

NASA Image and Video Library

2016-06-24

ISS Expedition 48-49 prime crewmembers Kate Rubins of NASA (left), Anatoly Ivanishin of Roscosmos (center) and Takuya Onishi of the Japan Aerospace Exploration Agency (right) pose for pictures with schoolchildren after arriving in Baikonur, Kazakhstan June 24 for final pre-launch training following a flight from Star City, Russia. The trio will launch July 7 from the Baikonur Cosmodrome in Kazakhstan on the Soyuz MS-01 spacecraft for a planned four-month mission on the International Space Station. NASA/Alexander Vysotsky

ISS Expedition 48-49 prime crewmembers Kate Rubins of NASA (left), Takuya Onishi of the Japan Aerospace Exploration Agency (center) and Anatoly Ivanishin of Roscosmos (right) wave to schoolchildren after arriving in Baikonur, Kazakhstan June 24 for final pre-launch training following a flight from Star City, Russia. The trio will launch July 7 from the Baikonur Cosmodrome in Kazakhstan on the Soyuz MS-01 spacecraft for a planned four-month mission on the International Space Station...NASA/Alexander Vysotsky.

NASA Image and Video Library

2016-06-24

ISS Expedition 48-49 prime crewmembers Kate Rubins of NASA (left), Takuya Onishi of the Japan Aerospace Exploration Agency (center) and Anatoly Ivanishin of Roscosmos (right) wave to schoolchildren after arriving in Baikonur, Kazakhstan June 24 for final pre-launch training following a flight from Star City, Russia. The trio will launch July 7 from the Baikonur Cosmodrome in Kazakhstan on the Soyuz MS-01 spacecraft for a planned four-month mission on the International Space Station. NASA/Alexander Vysotsky

De novo gene mutations highlight patterns of genetic and neural complexity in schizophrenia

PubMed Central

Xu, Bin; Ionita-Laza, Iuliana; Roos, J. Louw; Boone, Braden; Woodrick, Scarlet; Sun, Yan; Levy, Shawn; Gogos, Joseph A.; Karayiorgou, Maria

2013-01-01

To evaluate evidence for de novo etiologies in schizophrenia, we sequenced at high coverage the exomes of families recruited from two populations with distinct demographic structure and history. We sequenced a total of 795 exomes from 231 parent-proband trios enriched for sporadic schizophrenia cases, as well as 34 unaffected trios. We observed in cases an excess of non-synonymous single nucleotide variants as well as a higher prevalence of gene-disruptive de novo mutations. We found four genes (LAMA2, DPYD, TRRAP and VPS39) affected by recurrent de novo events within or across the two populations, a finding unlikely to have occurred by chance. We show that de novo mutations affect genes with diverse functions and developmental profiles but we also find a substantial contribution of mutations in genes with higher expression in early fetal life. Our results help define the pattern of genomic and neural architecture of schizophrenia. PMID:23042115

Catalog of Federal Education Assistance Programs--1976: An Indexed Guide to the Federal Government's Programs Offering Educational Benefits to the American People.

ERIC Educational Resources Information Center

Office of Education (DHEW), Washington, DC.

This catalog is composed of brief descriptions of, and extensive indexes to, federal programs that provide educational benefits to the American public. It includes programs administered by the U.S. Office of Education as well as those administered by other federal agencies. The federal education assistance programs are aimed at all educational…

34 CFR 682.100 - The Federal Family Education Loan programs.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false The Federal Family Education Loan programs. 682.100... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FEDERAL FAMILY EDUCATION LOAN (FFEL) PROGRAM Purpose and Scope § 682.100 The Federal Family Education Loan programs. (a) This part governs the following four programs...

Sporadic autism exomes reveal a highly interconnected protein network of de novo mutations.

PubMed

O'Roak, Brian J; Vives, Laura; Girirajan, Santhosh; Karakoc, Emre; Krumm, Niklas; Coe, Bradley P; Levy, Roie; Ko, Arthur; Lee, Choli; Smith, Joshua D; Turner, Emily H; Stanaway, Ian B; Vernot, Benjamin; Malig, Maika; Baker, Carl; Reilly, Beau; Akey, Joshua M; Borenstein, Elhanan; Rieder, Mark J; Nickerson, Deborah A; Bernier, Raphael; Shendure, Jay; Eichler, Evan E

2012-04-04

It is well established that autism spectrum disorders (ASD) have a strong genetic component; however, for at least 70% of cases, the underlying genetic cause is unknown. Under the hypothesis that de novo mutations underlie a substantial fraction of the risk for developing ASD in families with no previous history of ASD or related phenotypes--so-called sporadic or simplex families--we sequenced all coding regions of the genome (the exome) for parent-child trios exhibiting sporadic ASD, including 189 new trios and 20 that were previously reported. Additionally, we also sequenced the exomes of 50 unaffected siblings corresponding to these new (n = 31) and previously reported trios (n = 19), for a total of 677 individual exomes from 209 families. Here we show that de novo point mutations are overwhelmingly paternal in origin (4:1 bias) and positively correlated with paternal age, consistent with the modest increased risk for children of older fathers to develop ASD. Moreover, 39% (49 of 126) of the most severe or disruptive de novo mutations map to a highly interconnected β-catenin/chromatin remodelling protein network ranked significantly for autism candidate genes. In proband exomes, recurrent protein-altering mutations were observed in two genes: CHD8 and NTNG1. Mutation screening of six candidate genes in 1,703 ASD probands identified additional de novo, protein-altering mutations in GRIN2B, LAMC3 and SCN1A. Combined with copy number variant (CNV) data, these results indicate extreme locus heterogeneity but also provide a target for future discovery, diagnostics and therapeutics.

Genetic and expression analyses reveal elevated expression of syntaxin 1A ( STX1A) in high functioning autism.

PubMed

Nakamura, Kazuhiko; Anitha, Ayyappan; Yamada, Kazuo; Tsujii, Masatsugu; Iwayama, Yoshimi; Hattori, Eiji; Toyota, Tomoko; Suda, Shiro; Takei, Noriyoshi; Iwata, Yasuhide; Suzuki, Katsuaki; Matsuzaki, Hideo; Kawai, Masayoshi; Sekine, Yoshimoto; Tsuchiya, Kenji J; Sugihara, Gen-Ichi; Ouchi, Yasuomi; Sugiyama, Toshiro; Yoshikawa, Takeo; Mori, Norio

2008-12-01

Autism is a pervasive developmental disorder diagnosed in early childhood. Abnormalities of serotonergic neurotransmission have been reported in autism. Serotonin transporter (5-HTT), which modulates serotonin levels, is a major therapeutic target in autism. Therefore, factors that regulate 5-HTT expression might be implicated in autism. One candidate 5-HTT-regulatory protein is the presynaptic protein, syntaxin 1A (STX1A). We examined the association of STX1A with autism in a trio association study using DNA samples from 249 AGRE trios with autistic probands. Only male probands were selected, since autism is more prevalent among males. The probands of 102 trios had IQ>70, and were considered as high functioning autism (HFA). In transmission disequilibrium test (TDT) analysis, rs2293485 (p=0.034) and rs4717806 (p=0.033) showed nominal associations with HFA; modest haplotype association was also observed. The SNPs that showed associations were related to early developmental abnormalities (ADI-R_D). We further compared STX1A mRNA expression in the lymphocytes of drug-naive HFA patients (n=12) and age- and sex-matched controls (n=13). STX1A expression in the HFA group was significantly higher (p=0.001) than that of controls. Thus, we suggest a possible role of STX1A in the pathogenesis of HFA. During early childhood, there is a period of high brain serotonin synthesis that is disrupted in autistic children; STX1A might influence the serotonergic system during this stage of neurodevelopment, as implied by the association with ADI-R_D.

Contribution of Global Rare Copy-Number Variants to the Risk of Sporadic Congenital Heart Disease

PubMed Central

Soemedi, Rachel; Wilson, Ian J.; Bentham, Jamie; Darlay, Rebecca; Töpf, Ana; Zelenika, Diana; Cosgrove, Catherine; Setchfield, Kerry; Thornborough, Chris; Granados-Riveron, Javier; Blue, Gillian M.; Breckpot, Jeroen; Hellens, Stephen; Zwolinkski, Simon; Glen, Elise; Mamasoula, Chrysovalanto; Rahman, Thahira J.; Hall, Darroch; Rauch, Anita; Devriendt, Koenraad; Gewillig, Marc; O’ Sullivan, John; Winlaw, David S.; Bu’Lock, Frances; Brook, J. David; Bhattacharya, Shoumo; Lathrop, Mark; Santibanez-Koref, Mauro; Cordell, Heather J.; Goodship, Judith A.; Keavney, Bernard D.

2012-01-01

Previous studies have shown that copy-number variants (CNVs) contribute to the risk of complex developmental phenotypes. However, the contribution of global CNV burden to the risk of sporadic congenital heart disease (CHD) remains incompletely defined. We generated genome-wide CNV data by using Illumina 660W-Quad SNP arrays in 2,256 individuals with CHD, 283 trio CHD-affected families, and 1,538 controls. We found association of rare genic deletions with CHD risk (odds ratio [OR] = 1.8, p = 0.0008). Rare deletions in study participants with CHD had higher gene content (p = 0.001) with higher haploinsufficiency scores (p = 0.03) than they did in controls, and they were enriched with Wnt-signaling genes (p = 1 × 10−5). Recurrent 15q11.2 deletions were associated with CHD risk (OR = 8.2, p = 0.02). Rare de novo CNVs were observed in ∼5% of CHD trios; 10 out of 11 occurred on the paternally transmitted chromosome (p = 0.01). Some of the rare de novo CNVs spanned genes known to be involved in heart development (e.g., HAND2 and GJA5). Rare genic deletions contribute ∼4% of the population-attributable risk of sporadic CHD. Second to previously described CNVs at 1q21.1, deletions at 15q11.2 and those implicating Wnt signaling are the most significant contributors to the risk of sporadic CHD. Rare de novo CNVs identified in CHD trios exhibit paternal origin bias. PMID:22939634

Precise detection of de novo single nucleotide variants in human genomes.

PubMed

Gómez-Romero, Laura; Palacios-Flores, Kim; Reyes, José; García, Delfino; Boege, Margareta; Dávila, Guillermo; Flores, Margarita; Schatz, Michael C; Palacios, Rafael

2018-05-22

The precise determination of de novo genetic variants has enormous implications across different fields of biology and medicine, particularly personalized medicine. Currently, de novo variations are identified by mapping sample reads from a parent-offspring trio to a reference genome, allowing for a certain degree of differences. While widely used, this approach often introduces false-positive (FP) results due to misaligned reads and mischaracterized sequencing errors. In a previous study, we developed an alternative approach to accurately identify single nucleotide variants (SNVs) using only perfect matches. However, this approach could be applied only to haploid regions of the genome and was computationally intensive. In this study, we present a unique approach, coverage-based single nucleotide variant identification (COBASI), which allows the exploration of the entire genome using second-generation short sequence reads without extensive computing requirements. COBASI identifies SNVs using changes in coverage of exactly matching unique substrings, and is particularly suited for pinpointing de novo SNVs. Unlike other approaches that require population frequencies across hundreds of samples to filter out any methodological biases, COBASI can be applied to detect de novo SNVs within isolated families. We demonstrate this capability through extensive simulation studies and by studying a parent-offspring trio we sequenced using short reads. Experimental validation of all 58 candidate de novo SNVs and a selection of non-de novo SNVs found in the trio confirmed zero FP calls. COBASI is available as open source at https://github.com/Laura-Gomez/COBASI for any researcher to use. Copyright © 2018 the Author(s). Published by PNAS.

Transmission of human mtDNA heteroplasmy in the Genome of the Netherlands families: support for a variable-size bottleneck

PubMed Central

Li, Mingkun; Rothwell, Rebecca; Vermaat, Martijn; Wachsmuth, Manja; Schröder, Roland; Laros, Jeroen F.J.; van Oven, Mannis; de Bakker, Paul I.W.; Bovenberg, Jasper A.; van Duijn, Cornelia M.; van Ommen, Gert-Jan B.; Slagboom, P. Eline; Swertz, Morris A.; Wijmenga, Cisca; Kayser, Manfred; Boomsma, Dorret I.; Zöllner, Sebastian; de Knijff, Peter; Stoneking, Mark

2016-01-01

Although previous studies have documented a bottleneck in the transmission of mtDNA genomes from mothers to offspring, several aspects remain unclear, including the size and nature of the bottleneck. Here, we analyze the dynamics of mtDNA heteroplasmy transmission in the Genomes of the Netherlands (GoNL) data, which consists of complete mtDNA genome sequences from 228 trios, eight dizygotic (DZ) twin quartets, and 10 monozygotic (MZ) twin quartets. Using a minor allele frequency (MAF) threshold of 2%, we identified 189 heteroplasmies in the trio mothers, of which 59% were transmitted to offspring, and 159 heteroplasmies in the trio offspring, of which 70% were inherited from the mothers. MZ twin pairs exhibited greater similarity in MAF at heteroplasmic sites than DZ twin pairs, suggesting that the heteroplasmy MAF in the oocyte is the major determinant of the heteroplasmy MAF in the offspring. We used a likelihood method to estimate the effective number of mtDNA genomes transmitted to offspring under different bottleneck models; a variable bottleneck size model provided the best fit to the data, with an estimated mean of nine individual mtDNA genomes transmitted. We also found evidence for negative selection during transmission against novel heteroplasmies (in which the minor allele has never been observed in polymorphism data). These novel heteroplasmies are enhanced for tRNA and rRNA genes, and mutations associated with mtDNA diseases frequently occur in these genes. Our results thus suggest that the female germ line is able to recognize and select against deleterious heteroplasmies. PMID:26916109

Pedigree and genotyping quality analyses of over 10,000 DNA samples from the Generation Scotland: Scottish Family Health Study.

PubMed

Kerr, Shona M; Campbell, Archie; Murphy, Lee; Hayward, Caroline; Jackson, Cathy; Wain, Louise V; Tobin, Martin D; Dominiczak, Anna; Morris, Andrew; Smith, Blair H; Porteous, David J

2013-03-22

Generation Scotland: Scottish Family Health Study (GS:SFHS) is a family-based biobank of 24,000 participants with rich phenotype and DNA available for genetic research. This paper describes the laboratory results from genotyping 32 single nucleotide polymorphisms (SNPs) on DNA from over 10,000 participants who attended GS:SFHS research clinics. The analysis described here was undertaken to test the quality of genetic information available to researchers. The success rate of each marker genotyped (call rate), minor allele frequency and adherence to Mendelian inheritance are presented. The few deviations in marker transmission in the 925 parent-child trios analysed were assessed as to whether they were likely to be miscalled genotypes, data or sample handling errors, or pedigree inaccuracies including non-paternity. The first 10,450 GS:SFHS clinic participants who had spirometry and smoking data available and DNA extracted were selected. 32 SNPs were assayed, chosen as part of a replication experiment from a Genome-Wide Association Study meta-analysis of lung function. In total 325,336 genotypes were returned. The overall project pass rate (32 SNPs on 10,450 samples) was 97.29%. A total of 925 parent-child trios were assessed for transmission of the SNP markers, with 16 trios indicating evidence of inconsistency in the recorded pedigrees. The Generation Scotland: Scottish Family Health Study used well-validated study methods and can produce good quality genetic data, with a low error rate. The GS:SFHS DNA samples are of high quality and the family groups were recorded and processed with accuracy during collection of the cohort.

Bayes factors based on robust TDT-type tests for family trio design.

PubMed

Yuan, Min; Pan, Xiaoqing; Yang, Yaning

2015-06-01

Adaptive transmission disequilibrium test (aTDT) and MAX3 test are two robust-efficient association tests for case-parent family trio data. Both tests incorporate information of common genetic models including recessive, additive and dominant models and are efficient in power and robust to genetic model specifications. The aTDT uses information of departure from Hardy-Weinberg disequilibrium to identify the potential genetic model underlying the data and then applies the corresponding TDT-type test, and the MAX3 test is defined as the maximum of the absolute value of three TDT-type tests under the three common genetic models. In this article, we propose three robust Bayes procedures, the aTDT based Bayes factor, MAX3 based Bayes factor and Bayes model averaging (BMA), for association analysis with case-parent trio design. The asymptotic distributions of aTDT under the null and alternative hypothesis are derived in order to calculate its Bayes factor. Extensive simulations show that the Bayes factors and the p-values of the corresponding tests are generally consistent and these Bayes factors are robust to genetic model specifications, especially so when the priors on the genetic models are equal. When equal priors are used for the underlying genetic models, the Bayes factor method based on aTDT is more powerful than those based on MAX3 and Bayes model averaging. When the prior placed a small (large) probability on the true model, the Bayes factor based on aTDT (BMA) is more powerful. Analysis of a simulation data about RA from GAW15 is presented to illustrate applications of the proposed methods.

2012-2013 Federal Pell Grant Program End-of-Year Report

ERIC Educational Resources Information Center

Office of Postsecondary Education, US Department of Education, 2013

2013-01-01

The Federal Pell Grant End-of-Year Report presents primary aspects of Federal Pell Grant Program activity for the 2012-2013 award year. This presentation is a compilation of quantitative program data assembled to offer insights into the changes to the Title IV applicant universe and the Federal Pell Grant Program. The Federal Pell Grant…

2011-2012 Federal Pell Grant Program End-of-Year Report

ERIC Educational Resources Information Center

Office of Postsecondary Education, US Department of Education, 2012

2012-01-01

The Federal Pell Grant End-of-Year Report presents primary aspects of Federal Pell Grant Program activity for the 2011-2012 award year. This presentation is a compilation of quantitative program data assembled to offer insights into the changes to the Title IV applicant universe and the Federal Pell Grant Program. The Federal Pell Grant…

Trio of Topics: Questions of Mass, Electricity, and Time.

ERIC Educational Resources Information Center

Quantum, 1992

1992-01-01

Discusses three topics related to physics: (1) the center of mass of a long stick thrown horizontally; (2) how electric current flows in metals; and (3) the theory of relativity in relationship with fictionalized time machines. (MDH)

Joint testing of genotypic and gene-environment interaction identified novel association for BMP4 with non-syndromic CL/P in an Asian population using data from an International Cleft Consortium.

PubMed

Chen, Qianqian; Wang, Hong; Schwender, Holger; Zhang, Tianxiao; Hetmanski, Jacqueline B; Chou, Yah-Huei Wu; Ye, Xiaoqian; Yeow, Vincent; Chong, Samuel S; Zhang, Bo; Jabs, Ethylin Wang; Parker, Margaret M; Scott, Alan F; Beaty, Terri H

2014-01-01

Non-syndromic cleft lip with or without cleft palate (NSCL/P) is a common disorder with complex etiology. The Bone Morphogenetic Protein 4 gene (BMP4) has been considered a prime candidate gene with evidence accumulated from animal experimental studies, human linkage studies, as well as candidate gene association studies. The aim of the current study is to test for linkage and association between BMP4 and NSCL/P that could be missed in genome-wide association studies (GWAS) when genotypic (G) main effects alone were considered. We performed the analysis considering G and interactions with multiple maternal environmental exposures using additive conditional logistic regression models in 895 Asian and 681 European complete NSCL/P trios. Single nucleotide polymorphisms (SNPs) that passed the quality control criteria among 122 genotyped and 25 imputed single nucleotide variants in and around the gene were used in analysis. Selected maternal environmental exposures during 3 months prior to and through the first trimester of pregnancy included any personal tobacco smoking, any environmental tobacco smoke in home, work place or any nearby places, any alcohol consumption and any use of multivitamin supplements. A novel significant association held for rs7156227 among Asian NSCL/P and non-syndromic cleft lip and palate (NSCLP) trios after Bonferroni correction which was not seen when G main effects alone were considered in either allelic or genotypic transmission disequilibrium tests. Odds ratios for carrying one copy of the minor allele without maternal exposure to any of the four environmental exposures were 0.58 (95%CI = 0.44, 0.75) and 0.54 (95%CI = 0.40, 0.73) for Asian NSCL/P and NSCLP trios, respectively. The Bonferroni P values corrected for the total number of 117 tested SNPs were 0.0051 (asymptotic P = 4.39*10(-5)) and 0.0065 (asymptotic P = 5.54*10(-5)), accordingly. In European trios, no significant association was seen for any SNPs after Bonferroni corrections for the total number of 120 tested SNPs. Our findings add evidence from GWAS to support the role of BMP4 in susceptibility to NSCL/P originally identified in linkage and candidate gene association studies.

[Comparative analysis of 3D data visibility of the prepared tooth finishing line on a synthetic jaw model, captured by international scanners in a laboratory conditions].

PubMed

Ryakhovskiy, A N; Kostyukova, V V

The aim of the study was to compare accuracy of digital impression's finishing line and the zone under it taken by different intraoral scanning systems. Parameters of comparison were: different level of the finishing line to the gingiva and width of sulcus after retraction. For this purpose two synthetic jaw models with prepared teeth were scanned using intraoral scanning systems: 3D Progress (MHT S.P.A., IT - MHT Optic Research AG, CH); True Definition (3M ESPE, USA); Trios (3Shape A/S, DNK); CEREC AC Bluecam, CEREC Omnicam (Sirona Dental System GmbH, DE); Planscan (Planmeca, FIN) (each n=10). Reference-scanning was done by ATOS Core (GOM mbH, DE). The resulting digital impressions were superimposed with the master-scan. The lowest measured deviations (trueness) for intraoral scanners, where the finishing line was 0.5 mm above gingiva were with scanner True Definition - 18.8±6.63 (on the finishing line) and 51.0±14.33 µm (0.3 mm under the finishing line). In conditions where finishing line was on the same level with gingiva, scanner Trios showed the best results: 17.0±3.96 and 52.7±6.52 µm. When the finishing line was 0.5 mm under gingiva, none of the testing scanners could visualize the zone 0.3 mm lower the finishing line. The best results for accuracy o the finishing line in that circumstances showed Trios: 15.1±5.05 µm. The optimum visualization of the finishing line and the zone under it was reached when the sulcus was 0.3 mm after retraction. Thus, the best accuracy was obtained with Trios: 10.3±2.69 (on the finishing line) and 57.2±13.58 µm (0.3 mm under finishing line). The results show that intraoral scanners also provide enough accuracy for indicating finishing line and the zone under it in different conditions of preparation and gingiva retraction. However, not all of the testing scanners can properly indicate finishing line and the zone under it when shoulder is below gingiva and the width of sulcus is less than 0.2 mm.

Site Remediation Technology InfoBase: A Guide to Federal Programs, Information Resources, and Publications on Contaminated Site Cleanup Technologies. First Edition

NASA Technical Reports Server (NTRS)

1998-01-01

Table of Contents: Federal Cleanup Programs; Federal Site Remediation Technology Development Assistance Programs; Federal Site Remediation Technology Development Electronic Data Bases; Federal Electronic Resources for Site Remediation Technology Information; Other Electronic Resources for Site Remediation Technology Information; Other Electronic Resources for Site Remediation Technology Information; Selected Bibliography: Federal Publication on Alternative and Innovative Site Remediation; and Appendix: Technology Program Contacts.

View of Zero-G training for astronauts and payload specialists

NASA Image and Video Library

1984-08-27

Paul Scully-Power, 41-G payload specialist, links arms with two others as they experience weightlessness in the KC-135 training aircraft. The trio appears to be flying toward the front of the aircraft while others take photos.

Federal Employees Health Benefits Program and Federal Employees Dental and Vision Insurance Program: eligibility for Pathway Programs participants. Interim final rule with request for comments.

PubMed

2014-01-06

The U.S. Office of Personnel Management (OPM) is issuing an interim final regulation to update the Federal Employees Health Benefits Program (FEHBP) and the Federal Employees Dental and Vision Insurance Program (FEDVIP) regulations to reflect updated election opportunities for participants in the Pathways Programs. The Pathways Programs were created by Executive Order (E.O.) 13562, signed by the President on December 27, 2010, and are designed to enable the Federal Government to compete effectively for students and recent graduates by improving its recruitment efforts through internships and similar programs with Federal agencies. This interim final rule furthers these recruitment and retention efforts by providing health insurance, as well as dental and vision benefits, to eligible program participants and their families.

29 CFR 99.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2011 CFR

2011-07-01

... otherwise be at low-risk. (2) The phase of a Federal program in its life cycle at the Federal agency may... an established program with time-tested regulations. Also, significant changes in Federal programs, laws, regulations, or the provisions of contracts or grant agreements may increase risk. (3) The phase...

29 CFR 99.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Auditors § 99.525 Criteria for Federal program risk. (a) General. The auditor's determination should be... Federal program. The auditor shall use auditor judgment and consider criteria, such as described in... analysis, the auditor may wish to discuss a particular Federal program with auditee management and the...

7 CFR 3052.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2010 CFR

2010-01-01

... ORGANIZATIONS Auditors § 3052.525 Criteria for Federal program risk. (a) General. The auditor's determination... to the Federal program. The auditor shall use auditor judgment and consider criteria, such as... part of the risk analysis, the auditor may wish to discuss a particular Federal program with auditee...

75 FR 76691 - Oregon; Correction of Federal Authorization of the State's Hazardous Waste Management Program

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-09

...; Correction of Federal Authorization of the State's Hazardous Waste Management Program AGENCY: Environmental... to the State of Oregon's federally authorized RCRA hazardous waste management program. On January 7... changes the State of Oregon made to its federally authorized RCRA Hazardous Waste Management Program...

34 CFR 673.3 - Application.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., DEPARTMENT OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK-STUDY PROGRAM... Perkins Loan, FWS, and FSEOG Programs § 673.3 Application. (a) To participate in the Federal Perkins Loan... the Federal Perkins Loan, FWS, and FSEOG programs must be on a form approved by the Secretary and must...

34 CFR 673.3 - Application.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., DEPARTMENT OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK-STUDY PROGRAM... Perkins Loan, FWS, and FSEOG Programs § 673.3 Application. (a) To participate in the Federal Perkins Loan... the Federal Perkins Loan, FWS, and FSEOG programs must be on a form approved by the Secretary and must...

30 CFR 900.15 - Federal lands program cooperative agreements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... INTRODUCTION § 900.15 Federal lands program cooperative agreements. The full text of any State and Federal cooperative agreement for the regulation of coal exploration and mining on Federal lands is published below... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Federal lands program cooperative agreements...

30 CFR 900.15 - Federal lands program cooperative agreements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... INTRODUCTION § 900.15 Federal lands program cooperative agreements. The full text of any State and Federal cooperative agreement for the regulation of coal exploration and mining on Federal lands is published below... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Federal lands program cooperative agreements...

30 CFR 900.15 - Federal lands program cooperative agreements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... INTRODUCTION § 900.15 Federal lands program cooperative agreements. The full text of any State and Federal cooperative agreement for the regulation of coal exploration and mining on Federal lands is published below... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Federal lands program cooperative agreements...

30 CFR 900.15 - Federal lands program cooperative agreements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... INTRODUCTION § 900.15 Federal lands program cooperative agreements. The full text of any State and Federal cooperative agreement for the regulation of coal exploration and mining on Federal lands is published below... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Federal lands program cooperative agreements...

34 CFR 674.18 - Use of funds.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., DEPARTMENT OF EDUCATION FEDERAL PERKINS LOAN PROGRAM General Provisions § 674.18 Use of funds. (a) General. An institution shall deposit the funds it receives under the Federal Perkins Loan program into its... supplemental Federal Perkins Loan allocations for an award year to the Federal Work-Study program or Federal...

34 CFR 674.18 - Use of funds.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., DEPARTMENT OF EDUCATION FEDERAL PERKINS LOAN PROGRAM General Provisions § 674.18 Use of funds. (a) General. An institution shall deposit the funds it receives under the Federal Perkins Loan program into its... supplemental Federal Perkins Loan allocations for an award year to the Federal Work-Study program or Federal...

31 CFR 205.30 - What are the Federal oversight and compliance responsibilities?

Code of Federal Regulations, 2013 CFR

2013-07-01

... in the Catalog of Federal Domestic Assistance. (g) If a Federal Program Agency causes an interest... responsibilities? (a) A Federal Program Agency must designate an official representative to coordinate all... representative's name and title. A Federal Program Agency must notify us immediately of any change in the...

31 CFR 205.30 - What are the Federal oversight and compliance responsibilities?

Code of Federal Regulations, 2012 CFR

2012-07-01

... in the Catalog of Federal Domestic Assistance. (g) If a Federal Program Agency causes an interest... responsibilities? (a) A Federal Program Agency must designate an official representative to coordinate all... representative's name and title. A Federal Program Agency must notify us immediately of any change in the...

31 CFR 205.30 - What are the Federal oversight and compliance responsibilities?

Code of Federal Regulations, 2014 CFR

2014-07-01

... in the Catalog of Federal Domestic Assistance. (g) If a Federal Program Agency causes an interest... responsibilities? (a) A Federal Program Agency must designate an official representative to coordinate all... representative's name and title. A Federal Program Agency must notify us immediately of any change in the...

31 CFR 205.30 - What are the Federal oversight and compliance responsibilities?

Code of Federal Regulations, 2010 CFR

2010-07-01

... in the Catalog of Federal Domestic Assistance. (g) If a Federal Program Agency causes an interest... responsibilities? (a) A Federal Program Agency must designate an official representative to coordinate all... representative's name and title. A Federal Program Agency must notify us immediately of any change in the...

31 CFR 205.30 - What are the Federal oversight and compliance responsibilities?

Code of Federal Regulations, 2011 CFR

2011-07-01

... in the Catalog of Federal Domestic Assistance. (g) If a Federal Program Agency causes an interest... responsibilities? (a) A Federal Program Agency must designate an official representative to coordinate all... representative's name and title. A Federal Program Agency must notify us immediately of any change in the...

Federal Government Health, Education, and Welfare Programs of Assistance to American Indians Residing on Federal Reservations (Including Table of Contents and Index).

ERIC Educational Resources Information Center

Langone, Stephen A.

Federal health, education, and welfare programs for 1970 benefiting American Indians residing on Federal reservations are listed. The report is divided into 3 sections: (1) Federal Indian programs aimed at improving or providing Indian health services, tribal management services, housing, higher education, and conservation; (2) Federal programs…

Federal Student Aid Handbook, 2006-2007. Volume 6: Campus-Based Programs

ERIC Educational Resources Information Center

US Department of Education, 2006

2006-01-01

The Federal Perkins Loan, Federal Supplemental Educational Opportunity Grant (FSEOG), and Federal Work-Study (FWS) programs are called "campus-based" programs because each school is responsible for administering them on its own campus. This volume gives guidance on issues specific to the administration of the campus-based programs.…

10 CFR Appendix A to Subpart E of... - DOE Federally Assisted Programs Containing Age Distinctions

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false DOE Federally Assisted Programs Containing Age...) NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OR ACTIVITIES Nondiscrimination on the Basis of Age-Age... Federally Assisted Programs Containing Age Distinctions Statute, Name, Public Law, and U.S. Code Section and...

Preference and Priority in Federal Funding: Aligning Federal Resources to Maximize Program Investment Efficiency and Impacts in Communities

EPA Pesticide Factsheets

This page contains the document, Preference and Priority in Federal Funding: Aligning Federal Resources to Maximize Program Investment Efficiency and Impacts in Communities - Lessons from EPA’s Brownfields Program.

Catalog of Federal Education Assistance Programs--1978. An Indexed Guide to the Federal Government's Programs Offering Educational Benefits to the American People.

ERIC Educational Resources Information Center

Jasper, Renee B.

This catalog is composed of brief descriptions of and extensive indexes to federal programs that provide educational benefits to the American public. It includes all programs administered by the U.S. Office of Education as well as programs administered by other federal agencies in support of educational services, professional training, or library…

77 FR 38784 - Notice of Proposed Information Collection Requests; Federal Student Aid; Federal Perkins Loan...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-29

...; Federal Perkins Loan Program/NDSL Assignment Form SUMMARY: The Federal Perkins Loan Program allows for... exhausted all of its efforts in recovering an outstanding loan. The Perkins Assignment Form serves as the... to this notice will be considered public records. Title of Collection: Federal Perkins Loan Program...

77 FR 52705 - Notice of Submission for OMB Review; Federal Student Aid; Federal Perkins Loan Program/NDSL...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-30

... DEPARTMENT OF EDUCATION Notice of Submission for OMB Review; Federal Student Aid; Federal Perkins Loan Program/NDSL Assignment Form SUMMARY: The Federal Perkins Loan Program allows for assignment of... its efforts in recovering an outstanding loan. The Perkins Assignment Form serves as the transmittal...

COMMUNICATION THEORY - A WHITEHEADIAN BASIS,

DTIC Science & Technology

An attempt is made to determine the adequacy of Whitehead’s metaphysical scheme in interpreting a recent theory of human communication . The theory...is offered by a trio of psychologists who arrive at their insights of human behavior via the study of human communication . (Author)

29 CFR 99.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2012 CFR

2012-07-01

... otherwise be at low-risk. (2) The phase of a Federal program in its life cycle at the Federal agency may... of a Federal program in its life cycle at the auditee may indicate risk. For example, during the...

29 CFR 99.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2013 CFR

2013-07-01

... otherwise be at low-risk. (2) The phase of a Federal program in its life cycle at the Federal agency may... of a Federal program in its life cycle at the auditee may indicate risk. For example, during the...

29 CFR 99.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2014 CFR

2014-07-01

... otherwise be at low-risk. (2) The phase of a Federal program in its life cycle at the Federal agency may... of a Federal program in its life cycle at the auditee may indicate risk. For example, during the...

Information about Federal Assistance Listings and the Catalog of Federal Domestic Assistance

EPA Pesticide Factsheets

The primary purpose of the Catalog is to assist potential applicants in identifying programs which meet their specific objectives & to provide general information on Federal assistance programs. In 1977 Congress passed the Federal Program Information Act.

RNA-based determination of ESR1 and HER2 expression and response to neoadjuvant chemotherapy.

PubMed

Denkert, C; Loibl, S; Kronenwett, R; Budczies, J; von Törne, C; Nekljudova, V; Darb-Esfahani, S; Solbach, C; Sinn, B V; Petry, C; Müller, B M; Hilfrich, J; Altmann, G; Staebler, A; Roth, C; Ataseven, B; Kirchner, T; Dietel, M; Untch, M; von Minckwitz, G

2013-03-01

Hormone and human epidermal growth factor receptor 2 (HER2) receptors are the most important breast cancer biomarkers, and additional objective and quantitative test methods such as messenger RNA (mRNA)-based quantitative analysis are urgently needed. In this study, we investigated the clinical validity of RT-PCR-based evaluation of estrogen receptor (ESR1) and HER2 mRNA expression. A total of 1050 core biopsies from two retrospective (GeparTrio, GeparQuattro) and one prospective (PREDICT) neoadjuvant studies were evaluated by quantitative RT-PCR for ESR1 and HER2. ESR1 mRNA was significantly predictive for reduced response to neoadjuvant chemotherapy in univariate and multivariate analysis in all three cohorts. The complete pathologically documented response (pathological complete response, pCR) rate for ESR1+/HER2- tumors was 7.3%, 8.0% and 8.6%; for ESR1-/HER2- tumors it was 34.4%, 33.7% and 37.3% in GeparTrio, GeparQuattro and PREDICT, respectively (P < 0.001 in each cohort). In the Kaplan-Meier analysis in GeparTrio patients with ESR1+/HER2- tumors had the best prognosis, compared with ESR1-/HER2- and ESR1-/HER2+ tumors [disease-free survival (DFS): P < 0.0005, overall survival (OS): P < 0.0005]. Our results suggest that mRNA levels of ESR1 and HER2 predict response to neoadjuvant chemotherapy and are significantly associated with long-term outcome. As an additional option to standard immunohistochemistry and gene-array-based analysis, quantitative RT-PCR analysis might be useful for determination of the receptor status in breast cancer.

Comparison of Quantifiler(®) Trio and InnoQuant™ human DNA quantification kits for detection of DNA degradation in developed and aged fingerprints.

PubMed

Goecker, Zachary C; Swiontek, Stephen E; Lakhtakia, Akhlesh; Roy, Reena

2016-06-01

The development techniques employed to visualize fingerprints collected from crime scenes as well as post-development ageing may result in the degradation of the DNA present in low quantities in such evidence samples. Amplification of the DNA samples with short tandem repeat (STR) amplification kits may result in partial DNA profiles. A comparative study of two commercially available quantification kits, Quantifiler(®) Trio and InnoQuant™, was performed on latent fingerprint samples that were either (i) developed using one of three different techniques and then aged in ambient conditions or (ii) undeveloped and then aged in ambient conditions. The three fingerprint development techniques used were: cyanoacrylate fuming, dusting with black powder, and the columnar-thin-film (CTF) technique. In order to determine the differences between the expected quantities and actual quantities of DNA, manually degraded samples generated by controlled exposure of DNA standards to ultraviolet radiation were also analyzed. A total of 144 fingerprint and 42 manually degraded DNA samples were processed in this study. The results indicate that the InnoQuant™ kit is capable of producing higher degradation ratios compared to the Quantifiler(®) Trio kit. This was an expected result since the degradation ratio is a relative value specific for a kit based on the length and extent of amplification of the two amplicons that vary from one kit to the other. Additionally, samples with lower concentrations of DNA yielded non-linear relationships of degradation ratio with the duration of aging, whereas samples with higher concentrations of DNA yielded quasi-linear relationships. None of the three development techniques produced a noticeably different degradation pattern when compared to undeveloped fingerprints, and therefore do not impede downstream DNA analysis. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Comparative analysis on reproducibility among 5 intraoral scanners: sectional analysis according to restoration type and preparation outline form

PubMed Central

2016-01-01

PURPOSE The trueness and precision of acquired images of intraoral digital scanners could be influenced by restoration type, preparation outline form, scanning technology and the application of power. The aim of this study is to perform the comparative evaluation of the 3-dimensional reproducibility of intraoral scanners (IOSs). MATERIALS AND METHODS The phantom containing five prepared teeth was scanned by the reference scanner (Dental Wings) and 5 test IOSs (E4D dentist, Fastscan, iTero, Trios and Zfx Intrascan). The acquired images of the scanner groups were compared with the image from the reference scanner (trueness) and within each scanner groups (precision). Statistical analysis was performed using independent two-samples t-test and analysis of variance (α=.05). RESULTS The average deviations of trueness and precision of Fastscan, iTero and Trios were significantly lower than the other scanners. According to the restoration type, significantly higher trueness was observed in crown and inlay than in bridge. However, no significant difference was observed among four sites of preparation outline form. If compared by the characteristics of IOS, high trueness was observed in the group adopting the active triangulation and using powder. However, there was no significant difference between the still image acquisition and video acquisition groups. CONCLUSION Except for two intraoral scanners, Fastscan, iTero and Trios displayed comparable levels of trueness and precision values in tested phantom model. Difference in trueness was observed depending on the restoration type, the preparation outline form and characteristics of IOS, which should be taken into consideration when the intraoral scanning data are utilized. PMID:27826385

TNF-alpha-308G>A polymorphism and the risk of familial CAD in a Pakistani population.

PubMed

Hussain, Sabir; Iqbal, Tahir; Javed, Qamar

2015-01-01

A case-control and trio-families study was performed to establish a potential association between TNF-alpha gene promoter SNPs at -308 and -238, and occurrence of CAD in a Pakistani population. In the first phase, 150 patients and 150 controls were enrolled in the case-control association study. In the second phase, heritability of susceptible alleles was investigated from 88 trio-families with CAD affected offspring. Biochemical analysis of lipids and hs-CRP was carried out spectrophotometrically, while serum TNF-alpha concentrations were determined by enzyme-linked immunosorbent assay. Genotyping of the TNF-alpha SNPs were determined by PCR-RFLP method. Elevated serum TNF-alpha and hs-CRP were observed from CAD vs. controls (P<0.0001; for both). The evaluation of TNF-alpha-308G>A polymorphism in case-control study revealed that the said SNP was significantly associated with the increased risk of CAD. The findings demonstrated a significant link between the TNF-alpha variant allele A at -308 and CAD (P=0.0035), whereas the -238 SNP was not associated with the disease. Haplotype A-G of the TNF-alpha gene at -308G>A and -238G>A showed higher frequency in the patient group compared with controls (P<0.05). Moreover, data showed preferential transmission of the disease susceptible allele A at TNF-alpha-308 from parent to affected offspring in a trio-family study (P<0.0001). The current research leads to conclusion that the TNF-alpha-308G>A polymorphism is associated with CAD in the study population. Furthermore, for the first time, we showed that the TNF-alpha-308A allele was significantly associated with the familial CAD in our high risk population. Copyright © 2014. Published by Elsevier Inc.

Effect of ursodeoxycholic acid treatment on the altered progesterone and bile acid homeostasis in the mother-placenta-foetus trio during cholestasis of pregnancy

PubMed Central

Estiú, Maria C; Monte, Maria J; Rivas, Laura; Moirón, Maria; Gomez-Rodriguez, Laura; Rodriguez-Bravo, Tomas; Marin, Jose JG; Macias, Rocio IR

2015-01-01

Aim Intrahepatic cholestasis of pregnancy (ICP) is characterized by pruritus and elevated bile acid concentrations in maternal serum. This is accompanied by an enhanced risk of intra-uterine and perinatal complications. High concentrations of sulphated progesterone metabolites (PMS) have been suggested to be involved in the multifactorial aetiopathogenesis of ICP. The aim of this study was to investigate further the mechanism accounting for the beneficial effect of oral administration of ursodeoxycholic acid (UDCA), which is the standard treatment, regarding bile acid and PMS homeostasis in the mother-placenta-foetus trio. Method Using HPLC-MS/MS bile acids and PMS were determined in maternal and foetal serum and placenta. The expression of ABC proteins in placenta was determined by real time quantitative PCR (RT-QPCR) and immunofluorescence. Results In ICP, markedly increased concentrations of bile acids (tauroconjugates > glycoconjugates >> unconjugated), progesterone and PMS in placenta and maternal serum were accompanied by enhanced concentrations in foetal serum of bile acids, but not of PMS. UDCA treatment reduced bile acid accumulation in the mother-placenta-foetus trio, but had no significant effect on progesterone and PMS concentrations. ABCG2 mRNA abundance was increased in placentas from ICP patients vs. controls and remained stable following UDCA treatment, despite an apparent further increase in ABCG2. Conclusion UDCA administration partially reduces ICP-induced bile acid accumulation in mothers and foetuses despite the lack of effect on concentrations of progesterone and PMS in maternal serum. Up-regulation of placental ABCG2 may play an important role in protecting the foetus from high concentrations of bile acids and PMS during ICP. PMID:25099365

Effect of ursodeoxycholic acid treatment on the altered progesterone and bile acid homeostasis in the mother-placenta-foetus trio during cholestasis of pregnancy.

PubMed

Estiú, Maria C; Monte, Maria J; Rivas, Laura; Moirón, Maria; Gomez-Rodriguez, Laura; Rodriguez-Bravo, Tomas; Marin, Jose J G; Macias, Rocio I R

2015-02-01

Intrahepatic cholestasis of pregnancy (ICP) is characterized by pruritus and elevated bile acid concentrations in maternal serum. This is accompanied by an enhanced risk of intra-uterine and perinatal complications. High concentrations of sulphated progesterone metabolites (PMS) have been suggested to be involved in the multifactorial aetiopathogenesis of ICP. The aim of this study was to investigate further the mechanism accounting for the beneficial effect of oral administration of ursodeoxycholic acid (UDCA), which is the standard treatment, regarding bile acid and PMS homeostasis in the mother-placenta-foetus trio. Using HPLC-MS/MS bile acids and PMS were determined in maternal and foetal serum and placenta. The expression of ABC proteins in placenta was determined by real time quantitative PCR (RT-QPCR) and immunofluorescence. In ICP, markedly increased concentrations of bile acids (tauroconjugates > glycoconjugates > unconjugated), progesterone and PMS in placenta and maternal serum were accompanied by enhanced concentrations in foetal serum of bile acids, but not of PMS. UDCA treatment reduced bile acid accumulation in the mother-placenta-foetus trio, but had no significant effect on progesterone and PMS concentrations. ABCG2 mRNA abundance was increased in placentas from ICP patients vs. controls and remained stable following UDCA treatment, despite an apparent further increase in ABCG2. UDCA administration partially reduces ICP-induced bile acid accumulation in mothers and foetuses despite the lack of effect on concentrations of progesterone and PMS in maternal serum. Up-regulation of placental ABCG2 may play an important role in protecting the foetus from high concentrations of bile acids and PMS during ICP. © 2014 The British Pharmacological Society.

34 CFR 685.100 - The William D. Ford Federal Direct Loan Program.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false The William D. Ford Federal Direct Loan Program. 685...) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION WILLIAM D. FORD FEDERAL DIRECT LOAN PROGRAM Purpose and Scope § 685.100 The William D. Ford Federal Direct Loan Program. (a) Under the William D. Ford...

7 CFR 3052.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2013 CFR

2013-01-01

... operating unit (e.g., one college campus) or pervasive throughout the entity. (ii) When significant parts of... program in its life cycle at the Federal agency may indicate risk. For example, a new Federal program with... or grant agreements may increase risk. (3) The phase of a Federal program in its life cycle at the...

7 CFR 3052.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2012 CFR

2012-01-01

... operating unit (e.g., one college campus) or pervasive throughout the entity. (ii) When significant parts of... program in its life cycle at the Federal agency may indicate risk. For example, a new Federal program with... or grant agreements may increase risk. (3) The phase of a Federal program in its life cycle at the...

7 CFR 3052.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2014 CFR

2014-01-01

... operating unit (e.g., one college campus) or pervasive throughout the entity. (ii) When significant parts of... program in its life cycle at the Federal agency may indicate risk. For example, a new Federal program with... or grant agreements may increase risk. (3) The phase of a Federal program in its life cycle at the...

38 CFR 41.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2011 CFR

2011-07-01

... at low-risk. (2) The phase of a Federal program in its life cycle at the Federal agency may indicate... program in its life cycle at the auditee may indicate risk. For example, during the first and last years... section, to identify risk in Federal programs. Also, as part of the risk analysis, the auditor may wish to...

78 FR 5433 - Annual Notice of Interest Rates of Federal Student Loans Made Under the Federal Family Education...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-25

... Federal Family Education Loan Program AGENCY: Federal Student Aid, Department of Education. ACTION: Notice... under the Federal Family Education Loan (FFEL) Program. The Chief Operating Officer takes this action to give notice of FFEL Program loan interest rates to the public. DATES: This notice is effective January...

Federal Funding Sources for Rural Areas: Fiscal Year 1998. Rural Information Center Publications Series, No. 59. Revised Edition.

ERIC Educational Resources Information Center

Reynnells, M. Louise, Comp.

This publication lists 265 federal funding programs available to rural areas. The programs were selected from the Catalog of Federal Domestic Assistance, 1997, which is available online from FAPRS (the Federal Assistance Programs Retrieval System). Entries are listed under the following federal departments or agencies: Department of Agriculture,…

Federal Funding Sources for Rural Areas. Rural Information Center Publication Series, No. 49. Revised Edition.

ERIC Educational Resources Information Center

Reynnells, M. Louise, Comp.

This document lists 248 federal funding programs available to rural areas. The programs were selected from the Catalog of Federal Domestic Assistance, 1995, which is available online from the Federal Assistance Programs Retrieval System (FAPRS). Entries are listed under the following federal departments or agencies: Department of Agriculture,…

11 CFR 6.149 - Program accessibility: Discrimination prohibited.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 11 Federal Elections 1 2011-01-01 2011-01-01 false Program accessibility: Discrimination prohibited. 6.149 Section 6.149 Federal Elections FEDERAL ELECTION COMMISSION ENFORCEMENT OF NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE FEDERAL ELECTION COMMISSION...

34 CFR 673.1 - Purpose.

Code of Federal Regulations, 2011 CFR

2011-07-01

... OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK-STUDY PROGRAM, AND... amended (HEA) that participating institutions administer: (a) The Federal Perkins Loan Program, which...

34 CFR 673.1 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-07-01

... OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK-STUDY PROGRAM, AND... amended (HEA) that participating institutions administer: (a) The Federal Perkins Loan Program, which...

Genomic characteristics of cattle copy number variations

USDA-ARS?s Scientific Manuscript database

We performed a systematic analysis of cattle copy number variations (CNVs) using the Bovine HapMap SNP genotyping data, including 539 animals of 21 modern cattle breeds and 6 outgroups. After correcting genomic waves and considering the trio information, we identified 682 candidate CNV regions (CNVR...

Tangled trios? Characterizing a hybrid zone in Castilleja (Orobanchaceae)

Treesearch

Erika l. Hersch-Green; Richard Cronn

2009-01-01

Hybridization and polyploidization are exceedingly important processes because both influence the ecological envelope and evolutionary trajectory of land plants. These processes are frequently invoked for Castilleja (Indian paintbrushes) as contributors to morphological and genetic novelty and as complicating factors in species delimitations. Here...

Tyrosine phosphorylation and proteolytic cleavage of Notch are required for non-canonical Notch/Abl signaling in Drosophila axon guidance.

PubMed

Kannan, Ramakrishnan; Cox, Eric; Wang, Lei; Kuzina, Irina; Gu, Qun; Giniger, Edward

2018-01-17

Notch signaling is required for the development and physiology of nearly every tissue in metazoans. Much of Notch signaling is mediated by transcriptional regulation of downstream target genes, but Notch controls axon patterning in Drosophila by local modulation of Abl tyrosine kinase signaling, via direct interactions with the Abl co-factors Disabled and Trio. Here, we show that Notch-Abl axonal signaling requires both of the proteolytic cleavage events that initiate canonical Notch signaling. We further show that some Notch protein is tyrosine phosphorylated in Drosophila , that this form of the protein is selectively associated with Disabled and Trio, and that relevant tyrosines are essential for Notch-dependent axon patterning but not for canonical Notch-dependent regulation of cell fate. Based on these data, we propose a model for the molecular mechanism by which Notch controls Abl signaling in Drosophila axons. © 2018. Published by The Company of Biologists Ltd.

De Novo Coding Variants Are Strongly Associated with Tourette Disorder

PubMed Central

Willsey, A. Jeremy; Fernandez, Thomas V.; Yu, Dongmei; King, Robert A.; Dietrich, Andrea; Xing, Jinchuan; Sanders, Stephan J.; Mandell, Jeffrey D.; Huang, Alden Y.; Richer, Petra; Smith, Louw; Dong, Shan; Samocha, Kaitlin E.; Neale, Benjamin M.; Coppola, Giovanni; Mathews, Carol A.; Tischfield, Jay A.; Scharf, Jeremiah M.; State, Matthew W.; Heiman, Gary A.

2017-01-01

SUMMARY Whole-exome sequencing (WES) and de novo variant detection have proven a powerful approach to gene discovery in complex neurodevelopmental disorders. We have completed WES of 325 Tourette disorder trios from the Tourette International Collaborative Genetics cohort and a replication sample of 186 trios from the Tourette Syndrome Association International Consortium on Genetics (511 total). We observe strong and consistent evidence for the contribution of de novo likely gene-disrupting (LGD) variants (rate ratio [RR] 2.32, p = 0.002). Additionally, de novo damaging variants (LGD and probably damaging missense) are overrepresented in probands (RR 1.37, p = 0.003). We identify four likely risk genes with multiple de novo damaging variants in unrelated probands: WWC1 (WW and C2 domain containing 1), CELSR3 (Cadherin EGF LAG seven-pass G-type receptor 3), NIPBL (Nipped-B-like), and FN1 (fibronectin 1). Overall, we estimate that de novo damaging variants in approximately 400 genes contribute risk in 12% of clinical cases. PMID:28472652

77 FR 47374 - Notice of Proposed Information Collection Requests; Federal Student Aid; William D. Ford Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-08

... which borrowers in the William D. Ford Federal Direct Loan (Direct Loan) and Federal Family Education... DEPARTMENT OF EDUCATION Notice of Proposed Information Collection Requests; Federal Student Aid; William D. Ford Federal Direct Loan (Direct Loan) Program/ Federal Family Loan (FFEL) Program: Deferment...

76 FR 31312 - Federal Family Education Loan Program

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-31

... DEPARTMENT OF EDUCATION Federal Family Education Loan Program AGENCY: Federal Student Aid, Department of Education. ACTION: Notice inviting guaranty agencies to submit proposals to participate in a... participate in the Federal Family Education Loan (FFEL) Program to submit proposals to enter into a Voluntary...

34 CFR 673.7 - Administrative cost allowance.

Code of Federal Regulations, 2011 CFR

2011-07-01

... EDUCATION, DEPARTMENT OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK... Federal Perkins Loan, FWS, and FSEOG Programs § 673.7 Administrative cost allowance. (a) An institution participating in the Federal Perkins Loan, FWS, or FSEOG programs is entitled to an administrative cost...

34 CFR 673.7 - Administrative cost allowance.

Code of Federal Regulations, 2010 CFR

2010-07-01

... EDUCATION, DEPARTMENT OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK... Federal Perkins Loan, FWS, and FSEOG Programs § 673.7 Administrative cost allowance. (a) An institution participating in the Federal Perkins Loan, FWS, or FSEOG programs is entitled to an administrative cost...

41 CFR 101-30.101-17 - Supply support.

Code of Federal Regulations, 2014 CFR

2014-07-01

....101-17 Section 101-30.101-17 Public Contracts and Property Management Federal Property Management Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 30-FEDERAL CATALOG SYSTEM 30...; e.g., GSA stock program, Federal supply schedule program, GSA's buy-on-demand program, or GSA's...

41 CFR 101-30.101-17 - Supply support.

Code of Federal Regulations, 2013 CFR

2013-07-01

....101-17 Section 101-30.101-17 Public Contracts and Property Management Federal Property Management Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 30-FEDERAL CATALOG SYSTEM 30...; e.g., GSA stock program, Federal supply schedule program, GSA's buy-on-demand program, or GSA's...

41 CFR 101-30.101-17 - Supply support.

Code of Federal Regulations, 2012 CFR

2012-07-01

....101-17 Section 101-30.101-17 Public Contracts and Property Management Federal Property Management Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 30-FEDERAL CATALOG SYSTEM 30...; e.g., GSA stock program, Federal supply schedule program, GSA's buy-on-demand program, or GSA's...

41 CFR 101-30.101-17 - Supply support.

Code of Federal Regulations, 2010 CFR

2010-07-01

....101-17 Section 101-30.101-17 Public Contracts and Property Management Federal Property Management Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 30-FEDERAL CATALOG SYSTEM 30...; e.g., GSA stock program, Federal supply schedule program, GSA's buy-on-demand program, or GSA's...

41 CFR 101-30.101-17 - Supply support.

Code of Federal Regulations, 2011 CFR

2011-07-01

....101-17 Section 101-30.101-17 Public Contracts and Property Management Federal Property Management Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS SUPPLY AND PROCUREMENT 30-FEDERAL CATALOG SYSTEM 30...; e.g., GSA stock program, Federal supply schedule program, GSA's buy-on-demand program, or GSA's...

Information about the Catalog of Federal Domestic Assistance and EPA Programs

EPA Pesticide Factsheets

The primary purpose of the Catalog is to assist potential applicants in identifying programs which meet their specific objectives & to provide general information on Federal assistance programs. In 1977 Congress passed the Federal Program Information Act.

The intellectual developmental disorders Mexico study: situational diagnosis, burden, genomics and intervention proposal.

PubMed

Lazcano-Ponce, Eduardo; Katz, Gregorio; Rodríguez-Valentín, Rocío; Castro, Filipa de; Allen-Leigh, Betania; Márquez-Caraveo, María Elena; Ramírez-García, Miguel Ángel; Arroyo-García, Eduardo; Medina-Mora, María Elena; Ángeles, Gustavo; Urquieta-Salomón, José Edmundo; Salvador-Carulla, Luis

2016-01-01

This study aims to generate evidence on intellectual development disorders (IDD) in Mexico. IDD disease burden will be estimated with a probabilistic model, using population-based surveys. Direct and indirect costs of catastrophic expenses of families with a member with an IDD will be evaluated. Genomic characterization of IDD will include: sequencing participant exomes and performing bioinformatics analyses to identify de novo or inherited variants through trio analysis; identifying genetic variants associated with IDD, and validating randomly selected variants by polymerase chain reaction (PCR) and sequencing or real-time quantitative PCR (qPCR). Delphi surveys will be done on best practices for IDD diagnosis and management. An external evaluation will employ qualitative case studies of two social and labor inclusion programs for people with IDD. The results will constitute scientific evidence for the design, promotion and evaluation of public policies, which are currently absent on IDD.

H.R. 3221: Student Aid and Fiscal Responsibility Act of 2009. Congressional Budget Office Cost Estimate

ERIC Educational Resources Information Center

Congressional Budget Office, 2009

2009-01-01

H.R. 3221 would amend the Higher Education Act of 1965, which authorizes most federal postsecondary education programs. It would prohibit new federally guaranteed loans from being made under the Federal Family Education Loan (FFEL) Program and would increase direct spending for the Federal Pell Grant Program and other programs. The elimination of…

76 FR 14896 - Multi-Agency Informational Meeting Concerning Compliance With the Federal Select Agent Program...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-18

...] Multi-Agency Informational Meeting Concerning Compliance With the Federal Select Agent Program; Public... specific regulatory guidance related to the Federal Select Agent Program established under the Public.... Sarah Kwiatkowski, Veterinary Program Assistant, APHIS Select Agent Program, APHIS, 4700 River Road Unit...

76 FR 17617 - Multi-Agency Informational Meeting Concerning Compliance With the Federal Select Agent Program...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-30

...] Multi-Agency Informational Meeting Concerning Compliance With the Federal Select Agent Program; Public... specific regulatory guidance related to the Federal Select Agent Program established under the Public.... Sarah Kwiatkowski, Veterinary Program Assistant, APHIS Select Agent Program, APHIS, 4700 River Road Unit...

About the Federal Energy Management Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Richard Kidd

2009-04-23

Richard Kidd, Program Manager for the Federal Energy Management Program (FEMP), presents a discussion on FEMP direction and its future role, federal funding trends, future financing trends, and Earth Day observations.

24 CFR 100.302 - State and Federal elderly housing programs.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false State and Federal elderly housing... State and Federal elderly housing programs. The provisions regarding familial status in this part shall... specifically designed and operated to assist elderly persons, as defined in the State or Federal program. ...

24 CFR 100.302 - State and Federal elderly housing programs.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false State and Federal elderly housing... State and Federal elderly housing programs. The provisions regarding familial status in this part shall... specifically designed and operated to assist elderly persons, as defined in the State or Federal program. ...

Federal Agencies and Black Colleges.

ERIC Educational Resources Information Center

Federal Interagency Committee on Education, Washington, DC.

This is the third in a series of annual reports on the participation of the Nation's predominantly black colleges and universities in programs funded by federal agencies. The report documents the recent expansion and diversity of federal program support to these colleges. In this report, federal program funds are reported by agency for all…

14 CFR 151.15 - Federal-aid Airport Program: Policy affecting runway or taxiway remarking.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Federal-aid Airport Program: Policy... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS General Requirements § 151.15 Federal-aid Airport Program: Policy affecting runway or taxiway remarking. No project for...

14 CFR 151.15 - Federal-aid Airport Program: Policy affecting runway or taxiway remarking.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Federal-aid Airport Program: Policy... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS General Requirements § 151.15 Federal-aid Airport Program: Policy affecting runway or taxiway remarking. No project for...

14 CFR 151.15 - Federal-aid Airport Program: Policy affecting runway or taxiway remarking.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Federal-aid Airport Program: Policy... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIRPORTS FEDERAL AID TO AIRPORTS General Requirements § 151.15 Federal-aid Airport Program: Policy affecting runway or taxiway remarking. No project for...

The Federal Air Pollution Program.

ERIC Educational Resources Information Center

National Air Pollution Control Administration (DHEW), Washington, DC.

Described is the Federal air pollution program as it was in 1967. The booklet is divided into these major topics: History of the Federal Program; Research; Assistance to State and Local Governments; Abatement and Prevention of Air Pollution; Control of Motor Vehicle Pollution; Information and Education; and Conclusion. Federal legislation has…

48 CFR 22.804 - Affirmative action programs.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Affirmative action programs. 22.804 Section 22.804 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Affirmative action programs. ...

48 CFR 22.804 - Affirmative action programs.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Affirmative action programs. 22.804 Section 22.804 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Affirmative action programs. ...

Federal Education Funding: Overview of K-12 and Early Childhood Education Programs. Report to Congressional Requesters. GAO-10-51

ERIC Educational Resources Information Center

Scott, George A.

2010-01-01

Over the last two decades there have been a number of efforts to catalogue and determine how much is spent on federal education programs. However, because education programs are administered by agencies throughout the federal government, and there is no standard definition of a federal education program, there currently is no single source that…

Restructuring Military Medical Care

DTIC Science & Technology

1995-07-01

providers, perhaps under an approach such as the Federal Employees Health Benefits (FEHB) program , discussed later in this chapter. Effects on DoD’s...CARE July 1995 Military Family Association, would give beneficiaries access to care through the Federal Employees Health Benefits program as well as...enrollment levels and BOX 6. THE FEDERAL EMPLOYEES HEALTH BENEFITS PROGRAM The Federal Employees Health Benefits (FEHB) program is the source of health

Federal Facilities Programs.

ERIC Educational Resources Information Center

Chase, William W.

Brief descriptions are presented of a number of Office of Education federal funding programs directly supporting educational facilities planning and construction. Programs now in operation and several currently under consideration are pointed out. Consideration is also given to several programs in federal agencies other than the Office of…

Genomic and evolutionary characteristics of cattle copy number variations

USDA-ARS?s Scientific Manuscript database

We performed a systematic analysis of cattle copy number variations (CNVs) using the Bovine HapMap SNP genotyping data, including 539 animals of 21 modern cattle breeds and 6 outgroups. After correcting genomic waves and considering the trio information, we identified 682 candidate CNV regions (CNVR...

Genetics Home Reference: PURA syndrome

MedlinePlus

... lead to a reduced amount of functional Purα protein. Although it is not understood how a partial loss of Purα function leads to the ... KJ, Gawne-Cain M; DDD study, Magee AC, Turnpenny PD, Baralle D. Whole exome sequencing in family trios reveals de novo mutations in ...

The phloem-sap feeding mealybug (Ferrisia virgata) carries 'Candidatus Liberibacter asiaticus' populations without transmitting disease

USDA-ARS?s Scientific Manuscript database

Candidatus Liberibacter asiaticus (Las) is the primary causal agent of huanglongbing (HLB), the most devastating disease of citrus worldwide. Currently the known insect vectors of the HLB-associated bacteria are three species of psyllids, Diaphorina citri (Hemiptera: Sternorrhyncha: Psyllidae), Trio...

William D. Ford Federal Direct Loan Program. Final Regulations. Federal Register, Department of Education, 34 CFR Part 685

ERIC Educational Resources Information Center

National Archives and Records Administration, 2014

2014-01-01

The purpose of this regulatory action is to amend the regulations governing the William D. Ford Federal Direct Loan (Direct Loan) Program (§ 685.200 of title 34 of the Code of Federal Regulations (CFR)). These regulations strengthen and improve administration of the Federal Direct PLUS Loan Program authorized under title IV of the Higher Education…

Results from laboratory and field testing of nitrate measuring spectrophotometers

USGS Publications Warehouse

Snazelle, Teri T.

2015-01-01

In Phase II, the analyzers were deployed in field conditions at three diferent USGS sites. The measured nitrate concentrations were compared to discrete (reference) samples analyzed by the Direct UV method on a Shimadzu UV1800 bench top spectrophotometer, and by the National Environmental Methods Index (NEMI) method I-2548-11 at the USGS National Water Quality Laboratory. The first deployment at USGS site 0249620 on the East Pearl River in Hancock County, Mississippi, tested the ability of the TriOs ProPs (10-mm path length), Hach NITRATAX (5 mm), Satlantic SUNA (10 mm), and the S::CAN Spectro::lyser (5 mm) to accurately measure low-level (less than 2 mg-N/L) nitrate concentrations while observing the effect turbidity and colored dissolved organic matter (CDOM) would have on the analyzers' measurements. The second deployment at USGS site 01389005 Passaic River below Pompton River at Two Bridges, New Jersey, tested the analyzer's accuracy in mid-level (2-8 mg-N/L) nitrate concentrations. This site provided the means to test the analyzers' performance in two distinct matrices—the Passaic and the Pompton Rivers. In this deployment, three instruments tested in Phase I (TriOS, Hach, and SUNA) were deployed with the S::CAN Spectro::lyser (35 mm) already placed by the New Jersey Water Science Center (WSC). The third deployment at USGS site 05579610 Kickapoo Creek at 2100E Road near Bloomington, Illinois, tested the ability of the analyzers to measure high nitrate concentrations (greater than 8 mg-N/L) in turbid waters. For Kickapoo Creek, the HIF provided the TriOS (10 mm) and S::CAN (5 mm) from Phase I, and a SUNA V2 (5 mm) to be deployed adjacent to the Illinois WSC-owned Hach (2 mm). A total of 40 discrete samples were collected from the three deployment sites and analyzed. The nitrate concentration of the samples ranged from 0.3–22.2 mg-N/L. The average absolute difference between the TriOS measurements and discrete samples was 0.46 mg-N/L. For the combined data from the Hach 5-mm and 2-mm analyzers, the average absolute difference between the Hach samples and the discrete samples was 0.13 mg-N/L. For the SUNA and SUNA V2 combined data, the average absolute difference between the SUNA samples and the discrete samples was 0.66 mg-N/L. The average absolute difference between the S::CAN samples and the discrete samples was 0.63 mg-N/L.

5 CFR 890.601 - Coverage.

Code of Federal Regulations, 2012 CFR

2012-01-01

... EMPLOYEES HEALTH BENEFITS PROGRAM Transfers From Retired Federal Employees Health Benefits Program § 890.601... is eligible to enroll, under the Retired Federal Employees Health Benefits Program (part 891 of this chapter) is eligible to enroll under the Federal Employees Health Benefits Program under this part. [39 FR...

5 CFR 890.601 - Coverage.

Code of Federal Regulations, 2011 CFR

2011-01-01

... EMPLOYEES HEALTH BENEFITS PROGRAM Transfers From Retired Federal Employees Health Benefits Program § 890.601... is eligible to enroll, under the Retired Federal Employees Health Benefits Program (part 891 of this chapter) is eligible to enroll under the Federal Employees Health Benefits Program under this part. [39 FR...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 23 Highways 1 2013-04-01 2013-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 23 Highways 1 2014-04-01 2014-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 23 Highways 1 2012-04-01 2012-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

23 CFR 668.215 - Programming and project procedures.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

77 FR 66087 - Federal Perkins Loan Program, Federal Family Education Loan Program, and William D. Ford Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-01

...The Secretary amends the Federal Perkins Loan (Perkins Loan) program, Federal Family Education Loan (FFEL) program, and William D. Ford Federal Direct Loan (Direct Loan) program regulations. These final regulations implement a new Income-Contingent Repayment (ICR) plan in the Direct Loan program based on the President's ``Pay As You Earn'' repayment initiative, incorporate recent statutory changes to the Income-Based Repayment (IBR) plan in the Direct Loan and FFEL programs, and streamline and add clarity to the total and permanent disability (TPD) discharge process for borrowers in loan programs under title IV of the Higher Education Act of 1965, as amended (HEA). These final regulations implementing a new ICR plan and the statutory changes to the IBR plan will assist borrowers in repaying their loans while the changes to the TPD discharge process will reduce burden for borrowers who are disabled and seeking a discharge of their title IV debt.

How federalism shapes public health financing, policy, and program options.

PubMed

Ogden, Lydia L

2012-01-01

In the United States, fiscal and functional federalism strongly shape public health policy and programs. Federalism has implications for public health practice: it molds financing and disbursement options, including funding formulas, which affect allocations and program goals, and shapes how funding decisions are operationalized in a political context. This article explores how American federalism, both fiscal and functional, structures public health funding, policy, and program options, investigating the effects of intergovernmental transfers on public health finance and programs.

30 CFR 736.23 - Federal program effect on State law or regulations.

Code of Federal Regulations, 2010 CFR

2010-07-01

... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

30 CFR 736.23 - Federal program effect on State law or regulations.

Code of Federal Regulations, 2014 CFR

2014-07-01

... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

30 CFR 736.23 - Federal program effect on State law or regulations.

Code of Federal Regulations, 2013 CFR

2013-07-01

... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

30 CFR 736.23 - Federal program effect on State law or regulations.

Code of Federal Regulations, 2011 CFR

2011-07-01

... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

30 CFR 736.23 - Federal program effect on State law or regulations.

Code of Federal Regulations, 2012 CFR

2012-07-01

... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

34 CFR 685.101 - Participation in the Direct Loan Program.

Code of Federal Regulations, 2010 CFR

2010-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION WILLIAM D. FORD FEDERAL DIRECT LOAN PROGRAM Purpose and Scope... school participating in the Direct Loan Program may borrow under the Federal Direct Stafford/Ford Loan and Federal Direct Unsubsidized Stafford/Ford Loan Programs. An eligible graduate or professional...

30 CFR 903.700 - Arizona Federal program.

Code of Federal Regulations, 2010 CFR

2010-07-01

....700 Mineral Resources OFFICE OF SURFACE MINING RECLAMATION AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE ARIZONA § 903.700 Arizona Federal program. (a) This part establishes a Federal program under the Surface Mining Control and Reclamation Act...

Student Financial Aid. High-Risk Series.

ERIC Educational Resources Information Center

Comptroller General of the U.S., Washington, DC.

This publication discusses areas of concern in the Department of Education's management and oversight of postsecondary student financial aid programs, especially the Federal Family Education Loan Program (FFELP), the Federal Direct Student Loan Program (FDSLP), and the Federal Pell Grant Programs. The General Accounting Office determined that, in…

34 CFR 674.8 - Program participation agreement.

Code of Federal Regulations, 2011 CFR

2011-07-01

... EDUCATION, DEPARTMENT OF EDUCATION FEDERAL PERKINS LOAN PROGRAM General Provisions § 674.8 Program participation agreement. To participate in the Federal Perkins Loan program, an institution shall enter into a... that has been granted permission by the Secretary to participate in the ELO under the Federal Perkins...

34 CFR 674.8 - Program participation agreement.

Code of Federal Regulations, 2010 CFR

2010-07-01

... EDUCATION, DEPARTMENT OF EDUCATION FEDERAL PERKINS LOAN PROGRAM General Provisions § 674.8 Program participation agreement. To participate in the Federal Perkins Loan program, an institution shall enter into a... that has been granted permission by the Secretary to participate in the ELO under the Federal Perkins...

1998 statistical summaries : Federal Transit Administration : grant assistance programs

DOT National Transportation Integrated Search

1999-03-01

The 1998 Statistical Summaries provides information about the Federal Transit Administration's (FTA) major financial aid programs for Federal Fiscal Year (FY) 1998. The report covers the following programs: Urbanized Area Formula, Non-urbanized Area ...

5 CFR Appendix C to Subpart D of... - Application of Subpart D, Part 900, to Programs Receiving Federal Financial Assistance of the...

Code of Federal Regulations, 2010 CFR

2010-01-01

... Programs Receiving Federal Financial Assistance of the Office of Personnel Management C Appendix C to... Programs Receiving Federal Financial Assistance of the Office of Personnel Management Nondiscrimination in... subpart in programs receiving financial assistance under programs of the Office of Personnel Management...

A Guide to Federal Assistance Programs for Local School Systems.

ERIC Educational Resources Information Center

Fairley, Richard L.; Krumbein, Gerald

This manual is written to provide an overview of the Federal programs which are available at the local level and to aid school administrators in locating particular Federal programs that will fulfill specific educational needs. The guide is designed for use as a tool in planning and programing a comprehensive educational program, rather than as a…

34 CFR 668.61 - Recovery of funds.

Code of Federal Regulations, 2011 CFR

2011-07-01

... in which the institution disbursed Federal Pell Grant, ACG, National SMART Grant, Federal Perkins...-Study (FWS), Federal Perkins Loan, and Direct Subsidized Loan programs. Unsubsidized student financial...), the institution may not— (i) Disburse any Federal Pell Grant, FSEOG, or Federal Perkins Loan Program...

34 CFR 673.4 - Allocation and reallocation.

Code of Federal Regulations, 2011 CFR

2011-07-01

... EDUCATION, DEPARTMENT OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK... Federal Perkins Loan, FWS, and FSEOG Programs § 673.4 Allocation and reallocation. (a) Allocation and reallocation of Federal Perkins Loan funds. (1) The Secretary allocates Federal capital contributions to...

Mathematical Formulation of Multivariate Euclidean Models for Discrimination Methods.

ERIC Educational Resources Information Center

Mullen, Kenneth; Ennis, Daniel M.

1987-01-01

Multivariate models for the triangular and duo-trio methods are described, and theoretical methods are compared to a Monte Carlo simulation. Implications are discussed for a new theory of multidimensional scaling which challenges the traditional assumption that proximity measures and perceptual distances are monotonically related. (Author/GDC)

A Trio of Human Molecular Genetics PCR Assays

ERIC Educational Resources Information Center

Reinking, Jeffrey L.; Waldo, Jennifer T.; Dinsmore, Jannett

2013-01-01

This laboratory exercise demonstrates three different analytical forms of the polymerase chain reaction (PCR) that allow students to genotype themselves at four different loci. Here, we present protocols to allow students to a) genotype a non-coding polymorphic Variable Number of Tandem Repeat (VNTR) locus on human chromosome 5 using conventional…

The Unique Trio: Academic Achievement, Sport, and Gender

ERIC Educational Resources Information Center

Shachaf, Miri; Katz, Yaacov J.; Shoval, Ella

2013-01-01

This study examined gender, participation in sport and academic achievement of Israeli high school students. The study examined the academic achievement of those who participated in competitive or non-competitive sport and those who did not participate in sport. Results indicate that female athletes who participated in competitive sport attained…

Identifying new technologies that save energy and reduce costs to the Federal sector: The New Technology Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hunt, W.D.M.; Conover, D.R.; Stockmeyer, M.K.

1995-11-01

In 1990 the New Technology Demonstration Program (formerly the Test Bed Demonstration Program) was initiated by the US Department of Energy`s Office (DOE`s) of Federal Energy Management Programs with the purpose of accelerating the introduction of new technologies into the Federal sector. The program has since expanded into a multi-laboratory collaborative effort that evaluates new technologies and shares the results with the Federal design and procurement communities. These evaluations are performed on a collaborative basis which typically includes technology manufacturers, Federal facilities, utilities, trade associations, research institutes, and other in partnership with DOE. The end result is a range ofmore » effective technology transfer tools that provide operations and performance data on new technologies to Federal designers, building managers, and procurement officials. These tools assist in accelerating a technology`s Federal application and realizing reductions in energy consumption and costs.« less

Examining "One Grant, One Loan." NASFAA Task Force Report

ERIC Educational Resources Information Center

National Association of Student Financial Aid Administrators, 2016

2016-01-01

Growing concern over the complexity of the federal financial aid system and a push toward simplification has led to increased attention toward streamlining the federal student aid programs. Specifically, several proposals and policy papers have recommended consolidating the federal aid programs into one grant program and one loan program, commonly…

Marine Science Affairs--Selecting Priority Programs.

ERIC Educational Resources Information Center

National Council on Marine Resources and Engineering Development, Washington, DC.

This report summarizes accomplishments in 1969, describing Federal programs and policies, and new programs implemented to meet those policies. The report describes the priorities that have been selected in the Federal Marine Science program during 1969. The first chapter reviews the steps taken by the Federal Government during 1969 to advance and…

43 CFR 4.1355 - Burden of proof.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Special Rules Applicable to Surface Coal Mining Hearings and Appeals Request for Hearing on A Preliminary.... 1260(c) (federal Program; Federal Lands Program; Federal Program for Indian Lands) § 4.1355 Burden of... comply with the Act, its implementing regulations, the regulatory program, or the permit. [67 FR 61511...

7 CFR 3052.235 - Program-specific audits.

Code of Federal Regulations, 2010 CFR

2010-01-01

... program in a single audit. (2) The auditee shall prepare the financial statement(s) for the Federal... the financial statement(s) for the Federal program in accordance with GAGAS; (ii) Obtain an... opinion (or disclaimer of opinion) as to whether the financial statement(s) of the Federal program is...

Federal Programs for Libraries: A Directory. Second Edition.

ERIC Educational Resources Information Center

Leonard, Lawrence E., Comp.; Buchko, Michael, Jr., Comp.

Program information on nine federal library programs and 76 federal library-related programs is provided for use by libraries seeking funding sources for projects which have been proposed to meet their assessed needs. A grantsmanship bibliography is included, as well as five indexes: an applicant eligibility index, an authorization index, a…

Federal Programs Supporting Lifelong Learning.

ERIC Educational Resources Information Center

Christoffel, Pam, Comp.

Approximately 275 Federal programs support some form of lifelong learning. While the majority of the lifelong learning programs are administered by the various agencies within HEW, a significant number of programs are run by such Federal agencies as the Department of Justice, the National Foundation on the Arts and Humanities, and the Smithsonian…

48 CFR 19.1007 - Procedures.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Procedures. 19.1007 Section 19.1007 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Small Business Competitiveness Demonstration Program 19.1007 Procedures...

48 CFR 19.1004 - Participating agencies.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Participating agencies. 19.1004 Section 19.1004 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Small Business Competitiveness Demonstration Program 19.1004...

48 CFR 19.1006 - Exclusions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Exclusions. 19.1006 Section 19.1006 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Small Business Competitiveness Demonstration Program 19.1006 Exclusions...

48 CFR 19.1003 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Purpose. 19.1003 Section 19.1003 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Small Business Competitiveness Demonstration Program 19.1003 Purpose. The...

48 CFR 19.1002 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Definitions. 19.1002 Section 19.1002 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Small Business Competitiveness Demonstration Program 19.1002 Definitions...

Federal energy conservation programs. Perspectives from the public and private sectors. Volume 2: Public hearing, July 14 and 15, 1981, Washington, D.C.

NASA Astrophysics Data System (ADS)

1982-05-01

The federal governments' energy conservation programs were examined. Testimony regarding issues discussed at the hearing include: energy policy, benefits of energy conservation, state and local governments and private sector activities, successes and failures of federal programs, barriers to conservation and a continuing federal role.

40 CFR 93.165 - Early emission reduction credit programs at Federal facilities and installation subject to...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Early emission reduction credit... Federal Actions to State or Federal Implementation Plans § 93.165 Early emission reduction credit programs... the SIP or TIP in that area, create an early emissions reductions credit program. The Federal agency...

40 CFR 93.165 - Early emission reduction credit programs at Federal facilities and installation subject to...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Early emission reduction credit... Federal Actions to State or Federal Implementation Plans § 93.165 Early emission reduction credit programs... the SIP or TIP in that area, create an early emissions reductions credit program. The Federal agency...

Basic Program Elements for Federal employee Occupational Safety and Health Programs and related matters; Subpart I for Recordkeeping and Reporting Requirements. Final rule.

PubMed

2013-08-05

OSHA is issuing a final rule amending the Basic Program Elements to require Federal agencies to submit their occupational injury and illness recordkeeping information to the Bureau of Labor Statistics (BLS) and OSHA on an annual basis. The information, which is already required to be created and maintained by Federal agencies, will be used by BLS to aggregate injury and illness information throughout the Federal government. OSHA will use the information to identify Federal establishments with high incidence rates for targeted inspection, and assist in determining the most effective safety and health training for Federal employees. The final rule also interprets several existing basic program elements in our regulations to clarify requirements applicable to Federal agencies, amends the date when Federal agencies must submit to the Secretary of Labor their annual report on occupational safety and health programs, amends the date when the Secretary of Labor must submit to the President the annual report on Federal agency safety and health, and clarifies that Federal agencies must include uncompensated volunteers when reporting and recording occupational injuries and illnesses.

76 FR 70510 - Federal Employees' Group Life Insurance Program: New Federal Employees' Group Life Insurance...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-14

... OFFICE OF PERSONNEL MANAGEMENT Federal Employees' Group Life Insurance Program: New Federal Employees' Group Life Insurance (FEGLI) Premiums AGENCY: Office of Personnel Management. ACTION: Notice... [[Page 70511

28 CFR 42.503 - Discrimination prohibited.

Code of Federal Regulations, 2010 CFR

2010-07-01

... program or activity receiving Federal financial assistance. (b) Discriminatory actions prohibited. (1) A... program or activity receiving Federal financial assistance; (ii) Deny a qualified handicapped person an... program or activity receiving Federal financial assistance on the ground that other specialized aid...

48 CFR 19.504 - Inclusion of Federal Prison Industries, Inc.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Inclusion of Federal Prison Industries, Inc. 19.504 Section 19.504 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 19.504 Inclusion of...

48 CFR 19.504 - Inclusion of Federal Prison Industries, Inc.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Inclusion of Federal Prison Industries, Inc. 19.504 Section 19.504 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 19.504 Inclusion of...

48 CFR 19.504 - Inclusion of Federal Prison Industries, Inc.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Inclusion of Federal Prison Industries, Inc. 19.504 Section 19.504 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 19.504 Inclusion of...

48 CFR 19.504 - Inclusion of Federal Prison Industries, Inc.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Inclusion of Federal Prison Industries, Inc. 19.504 Section 19.504 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 19.504 Inclusion of...

78 FR 25282 - Agency Information Collection Activities: Proposed Collection; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-30

... Project: Mandatory Guidelines for Federal Workplace Drug Testing Programs (OMB No. 0930-0158)--Revision SAMHSA's Mandatory Guidelines for Federal Workplace Drug Testing Programs will request OMB approval for the Federal Drug Testing Custody and Control Form for federal agency and federally regulated drug...

48 CFR 19.504 - Inclusion of Federal Prison Industries, Inc.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Inclusion of Federal Prison Industries, Inc. 19.504 Section 19.504 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Set-Asides for Small Business 19.504 Inclusion of...

77 FR 29988 - Notice of Submission for OMB Review; Federal Student Aid; William D. Ford Federal Direct Loan...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-21

... benefits under the Department of Defense's Student Loan Repayment Program, or having a federal education... DEPARTMENT OF EDUCATION Notice of Submission for OMB Review; Federal Student Aid; William D. Ford... her William D. Ford Federal Direct Loan (Direct Loan) or Federal Family Education Loan (FFEL) Program...

30 CFR 937.700 - Oregon Federal program.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Oregon Federal program. 937.700 Section 937.700... PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE OREGON § 937.700 Oregon Federal program. (a) This part contains all rules that are applicable to surface coal mining operations in Oregon...

30 CFR 937.700 - Oregon Federal program.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Oregon Federal program. 937.700 Section 937.700... PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE OREGON § 937.700 Oregon Federal program. (a) This part contains all rules that are applicable to surface coal mining operations in Oregon...

30 CFR 937.700 - Oregon Federal program.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Oregon Federal program. 937.700 Section 937.700... PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE OREGON § 937.700 Oregon Federal program. (a) This part contains all rules that are applicable to surface coal mining operations in Oregon...

30 CFR 736.24 - Federal program effect on State funding.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Federal program effect on State funding. 736.24... § 736.24 Federal program effect on State funding. (a) After the withdrawal of a State program and the... finds, in writing, that discontinuation of funding would not be consistent with achieving the purposes...

30 CFR 736.24 - Federal program effect on State funding.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Federal program effect on State funding. 736.24... § 736.24 Federal program effect on State funding. (a) After the withdrawal of a State program and the... finds, in writing, that discontinuation of funding would not be consistent with achieving the purposes...

30 CFR 736.24 - Federal program effect on State funding.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Federal program effect on State funding. 736.24... § 736.24 Federal program effect on State funding. (a) After the withdrawal of a State program and the... finds, in writing, that discontinuation of funding would not be consistent with achieving the purposes...

30 CFR 736.24 - Federal program effect on State funding.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Federal program effect on State funding. 736.24... § 736.24 Federal program effect on State funding. (a) After the withdrawal of a State program and the... finds, in writing, that discontinuation of funding would not be consistent with achieving the purposes...

30 CFR 736.24 - Federal program effect on State funding.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Federal program effect on State funding. 736.24... § 736.24 Federal program effect on State funding. (a) After the withdrawal of a State program and the... finds, in writing, that discontinuation of funding would not be consistent with achieving the purposes...

Financing Early Childhood Education Programs: State, Federal, and Local Issues

ERIC Educational Resources Information Center

Hustedt, Jason T.; Barnett, W. Steven

2011-01-01

The landscape of financing early childhood education in the U.S. is complex. Programs run the gamut from tuition-supported private centers to public programs supported by federal, state, or local funds. Different funding streams are poorly coordinated. The federal government funds several major targeted programs that are available only to specific…

Environmental Quality Research and Development. A Review and Analysis of Federal Programs.

ERIC Educational Resources Information Center

Executive Office of the President, Washington, DC.

This is the first interagency report on Federal environmental quality research and development programs that presents program descriptions, levels of funding, and analyses. Undertaken at the request of the Federal Council for Science and Technology, the study may be useful in identifying environmental quality research and development programs in…

Federal Air Marshal Program; Federal Aviation Administration

DOT National Transportation Integrated Search

1997-04-17

The objective of the audit was to evaluate the effectiveness of Federal Aviation : Administration's (FAA) Federal Air Marshal (FAM) Program in providing for in : flight security requirements of high risk or special circumstance U.S.JairJcarrier fligh...

48 CFR 19.801 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false [Reserved] 19.801 Section 19.801 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Contracting With the Small Business Administration (the 8(a) Program) 19.801...

5 CFR 890.601 - Coverage.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) FEDERAL EMPLOYEES HEALTH BENEFITS PROGRAM Transfers From Retired Federal Employees Health Benefits Program § 890.601... is eligible to enroll, under the Retired Federal Employees Health Benefits Program (part 891 of this...

5 CFR 890.601 - Coverage.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) FEDERAL EMPLOYEES HEALTH BENEFITS PROGRAM Transfers From Retired Federal Employees Health Benefits Program § 890.601... is eligible to enroll, under the Retired Federal Employees Health Benefits Program (part 891 of this...

5 CFR 890.601 - Coverage.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) FEDERAL EMPLOYEES HEALTH BENEFITS PROGRAM Transfers From Retired Federal Employees Health Benefits Program § 890.601... is eligible to enroll, under the Retired Federal Employees Health Benefits Program (part 891 of this...

2 CFR 376.147 - Does an exclusion from participation in Federal health care programs under Title XI of the Social...

Code of Federal Regulations, 2010 CFR

2010-01-01

... Federal health care programs under Title XI of the Social Security Act affect a person's eligibility to..., Medicaid, and other Federal health care programs under Title XI of the Social Security Act, 42 U.S.C. 1320a... Federal Agency Regulations for Grants and Agreements DEPARTMENT OF HEALTH AND HUMAN SERVICES...

5 CFR 792.217 - Are part-time Federal employees eligible for the child care subsidy program?

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Are part-time Federal employees eligible... the Child Care Subsidy Program Legislation and to Whom Does It Apply? § 792.217 Are part-time Federal employees eligible for the child care subsidy program? Federal employees who work part-time are eligible for...

34 CFR 673.6 - Coordination with BIA grants.

Code of Federal Regulations, 2010 CFR

2010-07-01

... EDUCATION, DEPARTMENT OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK... Federal Perkins Loan, FWS, and FSEOG Programs § 673.6 Coordination with BIA grants. (a) Coordination of BIA grants with Federal Perkins loans, FWS awards, or FSEOGs. To determine the amount of a Federal...

34 CFR 673.6 - Coordination with BIA grants.

Code of Federal Regulations, 2011 CFR

2011-07-01

... EDUCATION, DEPARTMENT OF EDUCATION GENERAL PROVISIONS FOR THE FEDERAL PERKINS LOAN PROGRAM, FEDERAL WORK... Federal Perkins Loan, FWS, and FSEOG Programs § 673.6 Coordination with BIA grants. (a) Coordination of BIA grants with Federal Perkins loans, FWS awards, or FSEOGs. To determine the amount of a Federal...

31 CFR 205.2 - What definitions apply to this part?

Code of Federal Regulations, 2013 CFR

2013-07-01

... writing. Business day means a day when Federal Reserve Banks are open. Catalog of Federal Domestic... non-financial Federal assistance programs administered by agencies of the Federal government... Federal Program Agency is authorized by law to exercise judgment in awarding a grant and in selecting a...

31 CFR 205.2 - What definitions apply to this part?

Code of Federal Regulations, 2011 CFR

2011-07-01

... writing. Business day means a day when Federal Reserve Banks are open. Catalog of Federal Domestic... non-financial Federal assistance programs administered by agencies of the Federal government... Federal Program Agency is authorized by law to exercise judgment in awarding a grant and in selecting a...

31 CFR 205.2 - What definitions apply to this part?

Code of Federal Regulations, 2012 CFR

2012-07-01

... writing. Business day means a day when Federal Reserve Banks are open. Catalog of Federal Domestic... non-financial Federal assistance programs administered by agencies of the Federal government... Federal Program Agency is authorized by law to exercise judgment in awarding a grant and in selecting a...

31 CFR 205.2 - What definitions apply to this part?

Code of Federal Regulations, 2014 CFR

2014-07-01

... writing. Business day means a day when Federal Reserve Banks are open. Catalog of Federal Domestic... non-financial Federal assistance programs administered by agencies of the Federal government... Federal Program Agency is authorized by law to exercise judgment in awarding a grant and in selecting a...

75 FR 41488 - Agency Information Collection Activities: Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-16

.... Project: Mandatory Guidelines for Federal Workplace Drug Testing Programs (OMB No. 0930-0158)--Revision SAMHSA's Mandatory Guidelines for Federal Workplace Drug Testing Programs will request OMB approval for the Federal Drug Testing Custody and Control Form for Federal agency and federally regulated drug...

78 FR 67200 - Federal-State Unemployment Compensation Program: Certifications for 2013 Under the Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-08

... DEPARTMENT OF LABOR Employment and Training Administration Federal-State Unemployment Compensation Program: Certifications for 2013 Under the Federal Unemployment Tax Act AGENCY: Employment and Training... Federal Unemployment Tax Act, 26 U.S.C. 3301 et seq., thereby enabling employers who make contributions to...

75 FR 68001 - Federal-State Unemployment Compensation Program: Certifications for 2010 under the Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-04

... DEPARTMENT OF LABOR Employment and Training Administration Federal-State Unemployment Compensation Program: Certifications for 2010 under the Federal Unemployment Tax Act AGENCY: Employment and Training... Federal Unemployment Tax Act, 26 U.S.C. 3301 et seq., thereby enabling employers who make contributions to...

77 FR 66482 - Federal-State Unemployment Compensation Program: Certifications for 2012 Under the Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-05

... DEPARTMENT OF LABOR Employment and Training Administration Federal-State Unemployment Compensation Program: Certifications for 2012 Under the Federal Unemployment Tax Act AGENCY: Employment and Training... Federal Unemployment Tax Act, 26 U.S.C. 3301 et seq., thereby enabling employers who make contributions to...

76 FR 68790 - Federal-State Unemployment Compensation Program: Certifications for 2011 Under the Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-07

... DEPARTMENT OF LABOR Employment and Training Administration Federal-State Unemployment Compensation Program: Certifications for 2011 Under the Federal Unemployment Tax Act AGENCY: Employment and Training... Federal Unemployment Tax Act, 26 U.S.C. 3301 et seq., thereby enabling employers who make contributions to...

34 CFR 682.419 - Guaranty agency Federal Fund.

Code of Federal Regulations, 2010 CFR

2010-07-01

... EDUCATION, DEPARTMENT OF EDUCATION FEDERAL FAMILY EDUCATION LOAN (FFEL) PROGRAM Administration of the Federal Family Education Loan Programs by a Guaranty Agency § 682.419 Guaranty agency Federal Fund. (a... 34 Education 3 2010-07-01 2010-07-01 false Guaranty agency Federal Fund. 682.419 Section 682.419...

34 CFR 682.403 - Federal advances for claim payments.

Code of Federal Regulations, 2010 CFR

2010-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FEDERAL FAMILY EDUCATION LOAN (FFEL) PROGRAM Administration of the Federal Family Education Loan Programs by a Guaranty Agency § 682.403 Federal advances for claim... 34 Education 3 2010-07-01 2010-07-01 false Federal advances for claim payments. 682.403 Section...

Catalog of Federal Education Assistance Programs. An Indexed Guide to the Federal Government's Programs Offering Educational Benefits to the American People.

ERIC Educational Resources Information Center

Department of Health, Education, and Welfare, Washington, DC. Office of the Commissioner of Education.

This catalog cites all those programs administered by the U.S. Office of Education, as well as programs administered by other Federal agencies, in support of educational services, professional training, or library services available to the general public. Each program is described in terms of the specific type of assistance provided, the purpose…

Personal Responsibility and Work Opportunity Reconciliation Act of 1996 (PRWORA); interpretation of "federal public benefit"--HHS. Notice with comment period.

PubMed

1998-08-04

This notice with comment period interprets the term "Federal public benefit" as used in Title IV of the Personal Responsibility and Work Opportunity Reconciliation Act of 1996 (PRWORA), Pub. L. 104-193, and identifies the HHS programs that provide such benefits under this interpretation. According to section 401 if PRWORA, aliens who are not "qualified aliens" are not eligible for any "Federal public benefit," unless the "Federal public benefit" falls within a specified exception. A "Federal public benefit" includes "any grant, contract, loan, professional license, or commercial license" provided to an individual, and also "any retirement, welfare, health, disability, public or assisted housing, postsecondary education, food assistance, unemployment benefit, or any other similar benefit for which payments or assistance are provided to an individual, household, or family eligibility unit." Under section 432, providers of a non-exempt "Federal public benefit" must verify that a person applying for the benefit is a qualified alien and is eligible to receive the benefit. The HHS programs that provide "Federal public benefits" and are not otherwise excluded from the definition by the exceptions provided in section 401(b) are: Adoption Assistance Administration on Developmental Disabilities (ADD)-State Developmental Disabilities Councils (direct services only) ADD-Special Projects (direct services only) ADD-University Affiliated Programs (clinical disability assessment services only) Adult Programs/Payments to Territories Agency for Health Care Policy and Research Dissertation Grants Child Care and Development Fund Clinical Training Grant for Faculty Development in Alcohol & Drug Abuse Foster Care Health Profession Education and Training Assistance Independent Living Program Job Opportunities for Low Income Individuals (JOLI) Low Income Home Energy Assistance Program (LIHEAP) Medicare Medicaid (except assistance for an emergency medical condition) Mental Health Clinical Training Grants Native Hawaiian Loan Program Refugee Cash Assistance Refugee Medical Assistance Refugee Preventive Health Services Program Refugee Social Services Formula Program Refugee Social Services Discretionary Program Refugee Targeted Assistance Formula Program Refugee Targeted Assistance Discretionary Program Refugee Unaccompanied Minors Program Refugee Voluntary Agency Matching Grant Program Repatriation Program Residential Energy Assistance Challenge Option (REACH) Social Services Block Grant (SSBG) State Child Health Insurance Program (CHIP) Temporary Assistance for Needy Families (TANF) While all of these programs provide "Federal public benefits" this does not mean that all benefits or services provided under these programs are "Federal public benefits." As discussed in sections II and III below, some benefits or services under these programs may not be provided to an "individual, household, or family eligibility unit" and, therefore, do not constitute "Federal public benefits" as defined by PRWORA.

Transferring new technologies within the federal sector: The New Technology Demonstration Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Conover, D.R.; Hunt, D.M.

1994-08-01

The federal sector is the largest consumer of products in the United States and annually purchases almost 1.5 quads of energy measured at the building site at a cost of almost $10 billion (U.S. Department of Energy 1991). A review of design, construction, and procurement practices in the federal sector, as well as discussions with manufacturers and vendors, indicated that new technologies are not utilized in as timely a manner as possible. As a consequence of this technology transfer lag, the federal sector loses valuable energy and environmental benefits that can be derived through the application of new technologies. Inmore » addition, opportunities are lost to reduce federal energy expenditures and spur U.S. economic growth through the procurement of such technologies. In 1990, under the direction of the U.S. Department of Energy (DOE) Federal Energy Management Program, the Pacific Northwest Laboratory began the design of a program to accelerate the introduction of new U.S. technologies into the federal sector. Designated first as the Test Bed Demonstration Program and more recently the New Technology Demonstration Program, it sought to shorten the acceptance period of new technologies within the federal sector. By installing and evaluating various new technologies at federal facilities, the Program attempts to increase the acceptance of those new technologies through the results of {open_quotes}real-world{close_quotes} federal installations. Since that time, the Program has conducted new technology demonstrations and evaluations, evolved to address the need for more timely information transfer, and explored collaborative opportunities with other DOE offices and laboratories. This paper explains the processes by which a new technology demonstration project is implemented and presents a general description of the Program results to date.« less

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false FEGLI Program quality...

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false FEGLI Program quality...

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false FEGLI Program quality...

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false FEGLI Program quality...

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true FEGLI Program quality...

Quantifying the impacts of road construction on wetlands loss : preliminary analysis

DOT National Transportation Integrated Search

1997-06-10

Over the past decades, the role of federal programs in the generation of wetlands losses has received much attention. One of the federal programs most responsible for wetlands losses and degradation is believed to be the Federal Aid Highway Program. ...

Federal Student Loan Programs: Are They Meeting the Needs of Students and Schools? Hearing before the Committee on Government Reform, House of Representatives, One Hundred Ninth Congress, First Session (May 26, 2005). Serial Number 109-31

ERIC Educational Resources Information Center

US House of Representatives, 2005

2005-01-01

The Committee on Government Reform, chaired by Tom Davis (R-VA) convened this oversight hearing to examine Federal Student Loan Programs, specifically the management and performance of the Federal Family Education Loan Program and the William D. Ford Federal Direct Loan Program. The Committee focused on the Department of Education initiatives to…

Microgravity

NASA Image and Video Library

2000-12-15

Paul Ducheyne, a principal investigator in the microgravity materials science program and head of the University of Pernsylvania's Center for Bioactive Materials and Tissue Engineering, is leading the trio as they use simulated microgravity to determine the optimal characteristics of tiny glass particles for growing bone tissue. The result could make possible a much broader range of synthetic bone-grafting applications. Even in normal gravity, bioactive glass particles enhance bone growth in laboratory tests with flat tissue cultures. Ducheyne and his team believe that using the bioactive microcarriers in a rotating bioreactor in microgravity will produce improved, three-dimensional tissue cultures. The work is sponsored by NASA's Office of Biological and Physical Research. The bioreactor is managed by the Biotechnology Cell Science Program at NASA's Johnson Space Center (JSC). NASA-sponsored bioreactor research has been instrumental in helping scientists to better understand normal and cancerous tissue development. In cooperation with the medical community, the bioreactor design is being used to prepare better models of human colon, prostate, breast and ovarian tumors. Cartilage, bone marrow, heart muscle, skeletal muscle, pancreatic islet cells, liver and kidney are just a few of the normal tissues being cultured in rotating bioreactors by investigators. Credit: NASA and University of Pennsylvania Center for Bioactive Materials and Tissue Engineering.

Student Loans: Direct Loan Default. Report to Congressional Requesters.

ERIC Educational Resources Information Center

Bovbjerg, Barbara D.

This report presents data on default rates within two major federal student loan programs, the Federal Direct Loan Program (FDLP) and the Federal Family Education Loan Program (FFELP). Data from the National Student Loan Data System for 1997 and 1998 indicate that, overall, direct and guaranteed student loan programs had similar default rates--6.6…

Texas Nutrition Education and Training Program. Final Evaluation Report for Federal Fiscal Year 1994.

ERIC Educational Resources Information Center

Ahmad, Mahassen

This report presents federally mandated evaluations and needs assessments conducted by the Texas Nutrition Education and Training (NET) program during Federal Fiscal Year 1994. General program performance of NET reflected an increase in the number of children in Texas and expansion in NET program activities. Needs assessment data collected from…

77 FR 76414 - William D. Ford Federal Direct Loan Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-28

... DEPARTMENT OF EDUCATION 34 CFR Part 685 RIN 1840-AC94 [Docket ID ED-2008-OPE-0009] William D. Ford... Loan (Perkins Loan) Program; the Federal Family Education Loan (FFEL) Program; and the William D. Ford.... Accordingly, 34 CFR part 685 is corrected as follows: PART 685--WILLIAM D. FORD FEDERAL DIRECT LOAN PROGRAM 0...

Life after the ban: an assessment of US syringe exchange programs' attitudes about and early experiences with federal funding.

PubMed

Green, Traci C; Martin, Erika G; Bowman, Sarah E; Mann, Marita R; Beletsky, Leo

2012-05-01

We aimed to determine whether syringe exchange programs (SEPs) currently receive or anticipate pursuing federal funding and barriers to funding applications following the recent removal of the long-standing ban on using federal funds for SEPs. We conducted a telephone-administered cross-sectional survey of US SEPs. Descriptive statistics summarized responses; bivariate analyses examined differences in pursuing funding and experiencing barriers by program characteristics. Of the 187 SEPs (92.1%) that responded, 90.9% were legally authorized. Three received federal funds and 116 intended to pursue federal funding. Perceived federal funding barriers were common and included availability and accessibility of funds, legal requirements such as written police support, resource capacity to apply and comply with funding regulations, local political and structural organization, and concern around altering program culture. Programs without legal authorization, health department affiliation, large distribution, or comprehensive planning reported more federal funding barriers. Policy implementation gaps appear to render federal support primarily symbolic. In practice, funding opportunities may not be available to all SEPs. Increased technical assistance and legal reform could improve access to federal funds, especially for SEPs with smaller capacity and tenuous local support.

Resolved, That the Federal Government Should Fully Finance a Defensible Minimum Education for All Children in the Public Schools. An Affirmative Position.

ERIC Educational Resources Information Center

Hack, Walter G.

This report discusses several kinds of Federal aid to education and decides that a Federal foundation program is the best type. Arguments in favor of such a program are that (1) contemporary societal problems require a national policy (including a federally financed education program), (2) equity of financial responsibility for the educational…

Federal Personnel. Federal Agencies' Part-Time Employment Programs. Briefing Report to the Chairwoman, Task Force on Economic Security, Select Committee on Children, Youth and Families, House of Representatives.

ERIC Educational Resources Information Center

General Accounting Office, Washington, DC.

This report provides information on permanent part-time employment programs in the federal government. It contains statistical data showing general characteristics of the federal part-time work force and information about the part-time employment programs of the Office of Personnel Management (OPM), Veterans Administration, General Services…

The Economic and Social Condition of Rural America in the 1970's. Part 3: The Distribution of Federal Outlays Among U.S. Counties.

ERIC Educational Resources Information Center

Economic Research Service (USDA), Washington, DC.

The distribution of Federal outlays within the United States in fiscal year 1970 is summarized in this report. Selected Federal programs (242 programs comprising 74.4% of all Federal outlays) were divided into 4 program categories: human resource development; community development; agriculture and natural resources, and Defense, the National…

34 CFR 682.501 - Extent of Federal guarantee under the Federal GSL programs.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Extent of Federal guarantee under the Federal GSL programs. 682.501 Section 682.501 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FEDERAL FAMILY EDUCATION LOAN (FFEL...

76 FR 60850 - Rhode Island; Emergency and Related Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-30

...), including direct Federal assistance, under the Public Assistance program. This assistance excludes regular time costs for subgrantees' regular employees. Consistent with the requirement that Federal assistance...), including direct federal assistance, under the Public Assistance program. The following Catalog of Federal...

76 FR 34089 - Louisiana; Emergency and Related Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-10

... the Public Assistance program. This assistance excludes regular time costs for subgrantees' regular employees. Consistent with the requirement that Federal assistance is supplemental, any Federal funds... direct Federal assistance, under the Public Assistance program. The following Catalog of Federal Domestic...

76 FR 60851 - Maryland; Emergency and Related Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-30

...), including direct Federal assistance, under the Public Assistance program. This assistance excludes regular time costs for subgrantees' regular employees. Consistent with the requirement that Federal assistance... program at 75 percent Federal funding. The following Catalog of Federal Domestic Assistance Numbers (CFDA...

Federal Student Assistance and Categorical Programs. 1981 Edition.

ERIC Educational Resources Information Center

Balz, Frank J.

The fourth edition of analyses of selected federal student assistance and categorical programs prepared by the National Institute of Independent Colleges and Universities is presented. Sections include: (1) federal student assistance--undergraduate programs (Pell Grants, Supplemental Educational Opportunity Grants, State Student Incentive Grants,…

43 CFR 4.1361 - Who may file.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Special Rules Applicable to Surface Coal Mining Hearings and Appeals Request for Review of Approval Or... Sale of Rights Granted Under Permit (federal Program; Federal Lands Program; Federal Program for Indian... forth in § 4.1360 may file a request for review of that decision. ...

Understanding Project-Based Learning in Second Life with a Pedagogy, Training, and Assessment Trio

ERIC Educational Resources Information Center

Jarmon, Leslie; Traphagan, Tomoko; Mayrath, Michael

2008-01-01

This paper presents an empirical study of how Second Life (SL) was utilized for a highly successful project-based graduate interdisciplinary communication course. Researchers found that an integrated threefold approach emphasizing project-based pedagogy, technical training and support, and assessment/research was effective in cultivating and…

Intra-Sectoral Diversity: A Political Economy of Thai Private Higher Education

ERIC Educational Resources Information Center

Praphamontripong, Prachayani

2010-01-01

Private higher education (PHE) worldwide has been a rapid development in the last several decades. The private sector will continue to grow, diversify and undoubtedly play a significant role in the political economy of higher education. Nevertheless, systematically empirical studies on the trio relationships among PHE, institutional diversity and…

The Benefits of Making It Harder to Learn

ERIC Educational Resources Information Center

Lang, James M.

2012-01-01

In January 2011, a trio of researchers published the results of an experiment in which they demonstrated that students who read material in difficult, unfamiliar fonts learned it more deeply than students who read the same material in conventional, familiar fonts. Strange as that may seem, the finding stems from a well-established principle in…

Endangering Prosperity: A Global View of the American School

ERIC Educational Resources Information Center

Hanushek, Eric A.; Peterson, Paul E.; Woessmann, Ludger

2013-01-01

The relative deficiencies of U.S. public schools are a serious concern to parents and policymakers. But they should be of concern to all Americans, as a globalizing world introduces new competition for talent, markets, capital, and opportunity. In "Endangering Prosperity," a trio of experts on international education policy compares the…

Meeting Report: Incorporating Genomics Research into Undergraduate Curricula

ERIC Educational Resources Information Center

Dyer, Betsey Dexter; LeBlanc, Mark D.

2002-01-01

In the first of two National Science Foundation (NSF)-funded workshops, 30 professors of biology and computer science from 18 institutions met at Wheaton College in Norton, Massachusetts, on June 6-7, 2002, to share ideas on how to incorporate genomics research into undergraduate curricula. The participants included nine pairs or trios of…

Group Work in Science Classrooms

ERIC Educational Resources Information Center

McGregor, Debbie; Tolmie, Andrew

2009-01-01

This article considers how students might work together in small groups, from two to eight, in either a primary or secondary science classroom. The nature of group work can vary widely and could include, for example, a pair carrying out an illustrative experiment, a trio or quad debating climate change, or six or seven rehearsing how they will…

People with Disabilities. Federal Programs Could Work Together More Efficiently To Promote Employment. Report to the Chairman, Subcommittee on Employer-Employee Relations, Committee on Economic and Educational Opportunities. House of Representatives.

ERIC Educational Resources Information Center

General Accounting Office, Washington, DC. Health, Education, and Human Services Div.

This study identified federal programs designed to assist people with disabilities, with a special emphasis on programs promoting employment. The study focused on the extent to which information, eligibility, and services were coordinated under these programs and the efficiency of federal programs in promoting employment among people with…

Submonolayer Ag films on Fe(100): A first-principles analysis of energetics controlling adlayer thermodynamics and kinetics

DOE PAGES

Li, Wei; Huang, Li; Evans, James W.; ...

2016-04-11

Epitaxial growth of Ag on Fe(100) and postdeposition relaxation have been studied in several experiments. We provide a first-principles density functional theory analysis of key adatom interaction energies and diffusion barriers controlling growth and relaxation kinetics for the submonolayer regime, as these have not been assessed previously. A cluster expansion approach is used to obtain an extensive set of conventional lateral interactions between adatoms on fourfold hollow adsorption sites. We find robust oscillatory decay of pair interactions with increasing separation, and of trio interactions with increasing perimeter length. First- and second-nearest-neighbor pair interactions, as well as compact linear and bentmore » trio interactions, dominate. The adatom terrace diffusion barrier is estimated to be E d ≈ 0.39 eV. We also provide a limited analysis of unconventional interactions for which one adatom is at the bridge-site transition state for hopping and one or more others are at fourfold hollow sites. Furthermore, energy barriers for diffusion along island edges can be determined with the aid of both conventional and unconventional interactions.« less

De Novo Coding Variants Are Strongly Associated with Tourette Disorder.

PubMed

Willsey, A Jeremy; Fernandez, Thomas V; Yu, Dongmei; King, Robert A; Dietrich, Andrea; Xing, Jinchuan; Sanders, Stephan J; Mandell, Jeffrey D; Huang, Alden Y; Richer, Petra; Smith, Louw; Dong, Shan; Samocha, Kaitlin E; Neale, Benjamin M; Coppola, Giovanni; Mathews, Carol A; Tischfield, Jay A; Scharf, Jeremiah M; State, Matthew W; Heiman, Gary A

2017-05-03

Whole-exome sequencing (WES) and de novo variant detection have proven a powerful approach to gene discovery in complex neurodevelopmental disorders. We have completed WES of 325 Tourette disorder trios from the Tourette International Collaborative Genetics cohort and a replication sample of 186 trios from the Tourette Syndrome Association International Consortium on Genetics (511 total). We observe strong and consistent evidence for the contribution of de novo likely gene-disrupting (LGD) variants (rate ratio [RR] 2.32, p = 0.002). Additionally, de novo damaging variants (LGD and probably damaging missense) are overrepresented in probands (RR 1.37, p = 0.003). We identify four likely risk genes with multiple de novo damaging variants in unrelated probands: WWC1 (WW and C2 domain containing 1), CELSR3 (Cadherin EGF LAG seven-pass G-type receptor 3), NIPBL (Nipped-B-like), and FN1 (fibronectin 1). Overall, we estimate that de novo damaging variants in approximately 400 genes contribute risk in 12% of clinical cases. VIDEO ABSTRACT. Copyright © 2017 Elsevier Inc. All rights reserved.

Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas

PubMed Central

Hou, Yu; Guo, Huahu; Cao, Chen; Li, Xianlong; Hu, Boqiang; Zhu, Ping; Wu, Xinglong; Wen, Lu; Tang, Fuchou; Huang, Yanyi; Peng, Jirun

2016-01-01

Single-cell genome, DNA methylome, and transcriptome sequencing methods have been separately developed. However, to accurately analyze the mechanism by which transcriptome, genome and DNA methylome regulate each other, these omic methods need to be performed in the same single cell. Here we demonstrate a single-cell triple omics sequencing technique, scTrio-seq, that can be used to simultaneously analyze the genomic copy-number variations (CNVs), DNA methylome, and transcriptome of an individual mammalian cell. We show that large-scale CNVs cause proportional changes in RNA expression of genes within the gained or lost genomic regions, whereas these CNVs generally do not affect DNA methylation in these regions. Furthermore, we applied scTrio-seq to 25 single cancer cells derived from a human hepatocellular carcinoma tissue sample. We identified two subpopulations within these cells based on CNVs, DNA methylome, or transcriptome of individual cells. Our work offers a new avenue of dissecting the complex contribution of genomic and epigenomic heterogeneities to the transcriptomic heterogeneity within a population of cells. PMID:26902283

Replication study of Japanese cohorts supports the role of STX1A in autism susceptibility.

PubMed

Nakamura, Kazuhiko; Iwata, Yasuhide; Anitha, Ayyappan; Miyachi, Taishi; Toyota, Tomoko; Yamada, Satoru; Tsujii, Masatsugu; Tsuchiya, Kenji J; Iwayama, Yoshimi; Yamada, Kazuo; Hattori, Eiji; Matsuzaki, Hideo; Matsumoto, Kaori; Suzuki, Katsuaki; Suda, Shiro; Takebayashi, Kiyokazu; Takei, Nori; Ichikawa, Hironobu; Sugiyama, Toshiro; Yoshikawa, Takeo; Mori, Norio

2011-03-30

Autism is a pervasive developmental disorder diagnosed in early childhood. Abnormalities of serotonergic neurotransmission have been reported in autism. Serotonin transporter (5-HTT), which modulates serotonin levels, is a major therapeutic target in autism. Therefore, factors that regulate 5-HTT expression might be implicated in autism. One candidate 5-HTT-regulatory protein is the presynaptic protein, syntaxin 1A (STX1A). We examined the association of STX1A with autism in a trio association study using DNA samples from Japanese trios with autistic probands. In TDT analysis, rs69510130 (p=0.027) showed nominal associations with autism; modest haplotype association was also observed. We further compared STX1A mRNA expression between the autistic and control groups in the postmortem brain. In the anterior cingulate gyrus region, STX1A expression in the autism group was found to be significantly lower than that of the control group. Thus, we suggest a possible role of STX1A in the pathogenesis of autism. Copyright © 2010 Elsevier Inc. All rights reserved.

76 FR 61726 - North Carolina; Emergency and Related Determinations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-05

...), including direct Federal assistance, under the Public Assistance program. This assistance excludes regular time costs for subgrantees' regular employees. Consistent with the requirement that Federal assistance...), including direct federal assistance, under the Public Assistance program at 75 percent federal funding. The...

30 CFR 900.15 - Federal lands program cooperative agreements.

Code of Federal Regulations, 2010 CFR

2010-07-01

.... 900.15 Section 900.15 Mineral Resources OFFICE OF SURFACE MINING RECLAMATION AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR PROGRAMS FOR THE CONDUCT OF SURFACE MINING OPERATIONS WITHIN EACH STATE INTRODUCTION § 900.15 Federal lands program cooperative agreements. The full text of any State and Federal...

43 CFR 4.1351 - Preliminary finding by OSM.

Code of Federal Regulations, 2010 CFR

2010-10-01

... APPEALS PROCEDURES Special Rules Applicable to Surface Coal Mining Hearings and Appeals Request for...(c) of the Act, 30 U.s.c. 1260(c) (federal Program; Federal Lands Program; Federal Program for Indian... or has controlled surface coal mining and reclamation operations with a demonstrated pattern of...

31 CFR 205.11 - What requirements apply to funding techniques?

Code of Federal Regulations, 2014 CFR

2014-07-01

... Program Agency must minimize the time elapsing between the transfer of funds from the United States Treasury and the State's payout of funds for Federal assistance program purposes, whether the transfer... EFFICIENT FEDERAL-STATE FUNDS TRANSFERS Rules Applicable to Federal Assistance Programs Included in a...

31 CFR 205.24 - How are accurate estimates maintained?

Code of Federal Regulations, 2010 CFR

2010-07-01

... EFFICIENT FEDERAL-STATE FUNDS TRANSFERS Rules Applicable to Federal Assistance Programs Included in a... funding technique provisions in the Treasury-State agreement or take other mutually agreed upon corrective... funds to be transferred under the Federal assistance program or program component to which an estimate...

State Strategies to Help Schools Make the Most of Their National School Lunch Program. Issue Brief

ERIC Educational Resources Information Center

Mulheron, Joyal; Vonasek, Kara

2010-01-01

The National School Lunch Program (NSLP) is the second largest federally subsidized food assistance program, serving approximately 31 million lunches each day. Nearly all public and private schools offer the federally reimbursed school meals program, which cost the federal government $9.3 billion to operate in 2008. This Issue Brief highlights the…

Federal research and development for satellite communications

NASA Technical Reports Server (NTRS)

1977-01-01

A Committee on Satellite Communication (COSC) was formed under the auspices of the Space Applications Board (SAB) in order to study Federal research and development on satellite communications (SC). Discussion on whether to continue the research and development and the proper role of the Federal Government are addressed. Discussion focussed on six possible options for a Federal role in SC research and development: (1) the current NASA SC program; (2) an expanded NASA SC technology program; (3) a SC technology flight test support program; (4) an experimental SC technology flight program; (5) an experimental public service SC system program; and (6) an operational public service SC system program. Decision criteria and recommendations are presented.

38 CFR 41.520 - Major program determination.

Code of Federal Regulations, 2011 CFR

2011-07-01

... auditor shall identify the larger Federal programs, which shall be labeled Type A programs. Type A... programs not labeled Type A under paragraph (b)(1) of this section shall be labeled Type B programs. (3... programs as Type A programs. When a Federal program providing loans significantly affects the number or...

Rapid-Onset Obesity with Hypothalamic Dysfunction, Hypoventilation, and Autonomic Dysregulation (ROHHAD): exome sequencing of trios, monozygotic twins and tumours.

PubMed

Barclay, Sarah F; Rand, Casey M; Borch, Lauren A; Nguyen, Lisa; Gray, Paul A; Gibson, William T; Wilson, Richard J A; Gordon, Paul M K; Aung, Zaw; Berry-Kravis, Elizabeth M; Ize-Ludlow, Diego; Weese-Mayer, Debra E; Bech-Hansen, N Torben

2015-08-25

Rapid-onset Obesity with Hypothalamic Dysfunction, Hypoventilation, and Autonomic Dysregulation (ROHHAD) is thought to be a genetic disease caused by de novo mutations, though causative mutations have yet to be identified. We searched for de novo coding mutations among a carefully-diagnosed and clinically homogeneous cohort of 35 ROHHAD patients. We sequenced the exomes of seven ROHHAD trios, plus tumours from four of these patients and the unaffected monozygotic (MZ) twin of one (discovery cohort), to identify constitutional and somatic de novo sequence variants. We further analyzed this exome data to search for candidate genes under autosomal dominant and recessive models, and to identify structural variations. Candidate genes were tested by exome or Sanger sequencing in a replication cohort of 28 ROHHAD singletons. The analysis of the trio-based exomes found 13 de novo variants. However, no two patients had de novo variants in the same gene, and additional patient exomes and mutation analysis in the replication cohort did not provide strong genetic evidence to implicate any of these sequence variants in ROHHAD. Somatic comparisons revealed no coding differences between any blood and tumour samples, or between the two discordant MZ twins. Neither autosomal dominant nor recessive analysis yielded candidate genes for ROHHAD, and we did not identify any potentially causative structural variations. Clinical exome sequencing is highly unlikely to be a useful diagnostic test in patients with true ROHHAD. As ROHHAD has a high risk for fatality if not properly managed, it remains imperative to expand the search for non-exomic genetic risk factors, as well as to investigate other possible mechanisms of disease. In so doing, we will be able to confirm objectively the ROHHAD diagnosis and to contribute to our understanding of obesity, respiratory control, hypothalamic function, and autonomic regulation.

TRIO Platform: A Novel Low Profile In vivo Imaging Support and Restraint System for Mice.

PubMed

Voziyanov, Vladislav; Kemp, Benjamin S; Dressel, Chelsea A; Ponder, Kayla; Murray, Teresa A

2016-01-01

High resolution, in vivo optical imaging of the mouse brain over time often requires anesthesia, which necessitates maintaining the animal's body temperature and level of anesthesia, as well as securing the head in an optimal, stable position. Controlling each parameter usually requires using multiple systems. Assembling multiple components into the small space on a standard microscope stage can be difficult and some commercially available parts simply do not fit. Furthermore, it is time-consuming to position an animal in the identical position over multiple imaging sessions for longitudinal studies. This is especially true when using an implanted gradient index (GRIN) lens for deep brain imaging. The multiphoton laser beam must be parallel with the shaft of the lens because even a slight tilt of the lens can degrade image quality. In response to these challenges, we have designed a compact, integrated in vivo imaging support system to overcome the problems created by using separate systems during optical imaging in mice. It is a single platform that provides (1) sturdy head fixation, (2) an integrated gas anesthesia mask, and (3) safe warm water heating. This THREE-IN-ONE (TRIO) Platform has a small footprint and a low profile that positions a mouse's head only 20 mm above the microscope stage. This height is about one half to one third the height of most commercially available immobilization devices. We have successfully employed this system, using isoflurane in over 40 imaging sessions with an average of 2 h per session with no leaks or other malfunctions. Due to its smaller size, the TRIO Platform can be used with a wider range of upright microscopes and stages. Most of the components were designed in SOLIDWORKS® and fabricated using a 3D printer. This additive manufacturing approach also readily permits size modifications for creating systems for other small animals.

Detection of pathogenic copy number variants in children with idiopathic intellectual disability using 500 K SNP array genomic hybridization

PubMed Central

2009-01-01

Background Array genomic hybridization is being used clinically to detect pathogenic copy number variants in children with intellectual disability and other birth defects. However, there is no agreement regarding the kind of array, the distribution of probes across the genome, or the resolution that is most appropriate for clinical use. Results We performed 500 K Affymetrix GeneChip® array genomic hybridization in 100 idiopathic intellectual disability trios, each comprised of a child with intellectual disability of unknown cause and both unaffected parents. We found pathogenic genomic imbalance in 16 of these 100 individuals with idiopathic intellectual disability. In comparison, we had found pathogenic genomic imbalance in 11 of 100 children with idiopathic intellectual disability in a previous cohort who had been studied by 100 K GeneChip® array genomic hybridization. Among 54 intellectual disability trios selected from the previous cohort who were re-tested with 500 K GeneChip® array genomic hybridization, we identified all 10 previously-detected pathogenic genomic alterations and at least one additional pathogenic copy number variant that had not been detected with 100 K GeneChip® array genomic hybridization. Many benign copy number variants, including one that was de novo, were also detected with 500 K array genomic hybridization, but it was possible to distinguish the benign and pathogenic copy number variants with confidence in all but 3 (1.9%) of the 154 intellectual disability trios studied. Conclusion Affymetrix GeneChip® 500 K array genomic hybridization detected pathogenic genomic imbalance in 10 of 10 patients with idiopathic developmental disability in whom 100 K GeneChip® array genomic hybridization had found genomic imbalance, 1 of 44 patients in whom 100 K GeneChip® array genomic hybridization had found no abnormality, and 16 of 100 patients who had not previously been tested. Effective clinical interpretation of these studies requires considerable skill and experience. PMID:19917086

Complete-arch accuracy of intraoral scanners.

PubMed

Treesh, Joshua C; Liacouras, Peter C; Taft, Robert M; Brooks, Daniel I; Raiciulescu, Sorana; Ellert, Daniel O; Grant, Gerald T; Ye, Ling

2018-04-30

Intraoral scanners have shown varied results in complete-arch applications. The purpose of this in vitro study was to evaluate the complete-arch accuracy of 4 intraoral scanners based on trueness and precision measurements compared with a known reference (trueness) and with each other (precision). Four intraoral scanners were evaluated: CEREC Bluecam, CEREC Omnicam, TRIOS Color, and Carestream CS 3500. A complete-arch reference cast was created and printed using a 3-dimensional dental cast printer with photopolymer resin. The reference cast was digitized using a laboratory-based white light 3-dimensional scanner. The printed reference cast was scanned 10 times with each intraoral scanner. The digital standard tessellation language (STL) files from each scanner were then registered to the reference file and compared with differences in trueness and precision using a 3-dimensional modeling software. Additionally, scanning time was recorded for each scan performed. The Wilcoxon signed rank, Kruskal-Wallis, and Dunn tests were used to detect differences for trueness, precision, and scanning time (α=.05). Carestream CS 3500 had the lowest overall trueness and precision compared with Bluecam and TRIOS Color. The fourth scanner, Omnicam, had intermediate trueness and precision. All of the scanners tended to underestimate the size of the reference file, with exception of the Carestream CS 3500, which was more variable. Based on visual inspection of the color rendering of signed differences, the greatest amount of error tended to be in the posterior aspects of the arch, with local errors exceeding 100 μm for all scans. The single capture scanner Carestream CS 3500 had the overall longest scan times and was significantly slower than the continuous capture scanners TRIOS Color and Omnicam. Significant differences in both trueness and precision were found among the scanners. Scan times of the continuous capture scanners were faster than the single capture scanners. Published by Elsevier Inc.

TRIO Platform: A Novel Low Profile In vivo Imaging Support and Restraint System for Mice

PubMed Central

Voziyanov, Vladislav; Kemp, Benjamin S.; Dressel, Chelsea A.; Ponder, Kayla; Murray, Teresa A.

2016-01-01

High resolution, in vivo optical imaging of the mouse brain over time often requires anesthesia, which necessitates maintaining the animal's body temperature and level of anesthesia, as well as securing the head in an optimal, stable position. Controlling each parameter usually requires using multiple systems. Assembling multiple components into the small space on a standard microscope stage can be difficult and some commercially available parts simply do not fit. Furthermore, it is time-consuming to position an animal in the identical position over multiple imaging sessions for longitudinal studies. This is especially true when using an implanted gradient index (GRIN) lens for deep brain imaging. The multiphoton laser beam must be parallel with the shaft of the lens because even a slight tilt of the lens can degrade image quality. In response to these challenges, we have designed a compact, integrated in vivo imaging support system to overcome the problems created by using separate systems during optical imaging in mice. It is a single platform that provides (1) sturdy head fixation, (2) an integrated gas anesthesia mask, and (3) safe warm water heating. This THREE-IN-ONE (TRIO) Platform has a small footprint and a low profile that positions a mouse's head only 20 mm above the microscope stage. This height is about one half to one third the height of most commercially available immobilization devices. We have successfully employed this system, using isoflurane in over 40 imaging sessions with an average of 2 h per session with no leaks or other malfunctions. Due to its smaller size, the TRIO Platform can be used with a wider range of upright microscopes and stages. Most of the components were designed in SOLIDWORKS® and fabricated using a 3D printer. This additive manufacturing approach also readily permits size modifications for creating systems for other small animals. PMID:27199633

NPPB and ACAN, Two Novel SHOX2 Transcription Targets Implicated in Skeletal Development

PubMed Central

Hisado-Oliva, Alfonso; Belinchón, Alberta; Gorbenko-del Blanco, Darya; Rodriguez, Jose Ignacio; Benito-Sanz, Sara; Campos-Barros, Angel; Heath, Karen E.

2014-01-01

SHOX and SHOX2 transcription factors are highly homologous, with even identical homeodomains. Genetic alterations in SHOX result in two skeletal dysplasias; Léri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia (LMD), while no human genetic disease has been linked to date with SHOX2. SHOX2 is, though, involved in skeletal development, as shown by different knockout mice models. Due to the high homology between SHOX and SHOX2, and their functional redundancy during heart development, we postulated that SHOX2 might have the same transcriptional targets and cofactors as SHOX in limb development. We selected two SHOX transcription targets regulated by different mechanisms: 1) the natriuretic peptide precursor B gene (NPPB) involved in the endochondral ossification signalling and directly activated by SHOX; and 2) Aggrecan (ACAN), a major component of cartilage extracellular matrix, regulated by the cooperation of SHOX with the SOX trio (SOX5, SOX6 and SOX9) via the protein interaction between SOX5/SOX6 and SHOX. Using the luciferase assay we have demonstrated that SHOX2, like SHOX, regulates NPPB directly whilst activates ACAN via its cooperation with the SOX trio. Subsequently, we have identified and characterized the protein domains implicated in the SHOX2 dimerization and also its protein interaction with SOX5/SOX6 and SHOX using the yeast-two hybrid and co-immunoprecipitation assays. Immunohistochemistry of human fetal growth plates from different time points demonstrated that SHOX2 is coexpressed with SHOX and the members of the SOX trio. Despite these findings, no mutation was identified in SHOX2 in a cohort of 83 LWD patients with no known molecular defect, suggesting that SHOX2 alterations do not cause LWD. In conclusion, our work has identified the first cofactors and two new transcription targets of SHOX2 in limb development, and we hypothesize a time- and tissue-specific functional redundancy between SHOX and SHOX2. PMID:24421874

NPPB and ACAN, two novel SHOX2 transcription targets implicated in skeletal development.

PubMed

Aza-Carmona, Miriam; Barca-Tierno, Veronica; Hisado-Oliva, Alfonso; Belinchón, Alberta; Gorbenko-del Blanco, Darya; Rodriguez, Jose Ignacio; Benito-Sanz, Sara; Campos-Barros, Angel; Heath, Karen E

2014-01-01

SHOX and SHOX2 transcription factors are highly homologous, with even identical homeodomains. Genetic alterations in SHOX result in two skeletal dysplasias; Léri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia (LMD), while no human genetic disease has been linked to date with SHOX2. SHOX2 is, though, involved in skeletal development, as shown by different knockout mice models. Due to the high homology between SHOX and SHOX2, and their functional redundancy during heart development, we postulated that SHOX2 might have the same transcriptional targets and cofactors as SHOX in limb development. We selected two SHOX transcription targets regulated by different mechanisms: 1) the natriuretic peptide precursor B gene (NPPB) involved in the endochondral ossification signalling and directly activated by SHOX; and 2) Aggrecan (ACAN), a major component of cartilage extracellular matrix, regulated by the cooperation of SHOX with the SOX trio (SOX5, SOX6 and SOX9) via the protein interaction between SOX5/SOX6 and SHOX. Using the luciferase assay we have demonstrated that SHOX2, like SHOX, regulates NPPB directly whilst activates ACAN via its cooperation with the SOX trio. Subsequently, we have identified and characterized the protein domains implicated in the SHOX2 dimerization and also its protein interaction with SOX5/SOX6 and SHOX using the yeast-two hybrid and co-immunoprecipitation assays. Immunohistochemistry of human fetal growth plates from different time points demonstrated that SHOX2 is coexpressed with SHOX and the members of the SOX trio. Despite these findings, no mutation was identified in SHOX2 in a cohort of 83 LWD patients with no known molecular defect, suggesting that SHOX2 alterations do not cause LWD. In conclusion, our work has identified the first cofactors and two new transcription targets of SHOX2 in limb development, and we hypothesize a time- and tissue-specific functional redundancy between SHOX and SHOX2.

Loss of syd-1 from R7 Neurons Disrupts Two Distinct Phases of Presynaptic Development

PubMed Central

Holbrook, Scott; Finley, Jennifer K.; Lyons, Eric L.

2012-01-01

Genetic analyses in both worm and fly have identified the RhoGAP-like protein Syd-1 as a key positive regulator of presynaptic assembly. In worm, loss of syd-1 can be fully rescued by overexpressing wild-type Liprin-α, suggesting that the primary function of Syd-1 in this process is to recruit Liprin-α. We show that loss of syd-1 from Drosophila R7 photoreceptors causes two morphological defects that occur at distinct developmental time points. First, syd-1 mutant R7 axons often fail to form terminal boutons in their normal M6 target layer. Later, those mutant axons that do contact M6 often project thin extensions beyond it. We find that the earlier defect coincides with a failure to localize synaptic vesicles, suggesting that it reflects a failure in presynaptic assembly. We then analyze the relationship between syd-1 and Liprin-α in R7s. We find that loss of Liprin-α causes a stronger early R7 defect and provide a possible explanation for this disparity: we show that Liprin-α promotes Kinesin-3/Unc-104/Imac-mediated axon transport independently of Syd-1 and that Kinesin-3/Unc-104/Imac is required for normal R7 bouton formation. Unlike loss of syd-1, loss of Liprin-α does not cause late R7 extensions. We show that overexpressing Liprin-α partly rescues the early but not the late syd-1 mutant R7 defect. We therefore conclude that the two defects are caused by distinct molecular mechanisms. We find that Trio overexpression rescues both syd-1 defects and that trio and syd-1 have similar loss- and gain-of-function phenotypes, suggesting that the primary function of Syd-1 in R7s may be to promote Trio activity. PMID:23238725

77 FR 58819 - Notice of Submission for OMB Review; Federal Student Aid; William D. Ford Federal Direct Loan...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-24

... DEPARTMENT OF EDUCATION Notice of Submission for OMB Review; Federal Student Aid; William D. Ford... William D. Ford Federal Direct Loan Program will use this form to request forbearance on their loans when... of Collection: William D. Ford Federal Direct Loan Program General Forbearance Request. OMB Control...

76 FR 74063 - Mandatory Guidelines for Federal Workplace Drug Testing Programs

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-30

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Mandatory Guidelines for Federal Workplace Drug Testing... Federal Workplace Drug Testing Programs (Mandatory Guidelines), effective on October 1, 2010, addresses... for physicians performing a review of Federal employee drug testing results that seek approval by the...

78 FR 63464 - William D. Ford Federal Direct Loan Program Repayment Plan Selection Form; Extension of Public...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-24

... DEPARTMENT OF EDUCATION William D. Ford Federal Direct Loan Program Repayment Plan Selection Form; Extension of Public Comment Period; Correction AGENCY: Department of Education. ACTION: Correction notice... entitled, ``William D. Ford Federal Direct Loan Program Repayment Plan Selection Form''. ED is extending...

38 CFR 41.525 - Criteria for Federal program risk.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) AUDITS OF STATES, LOCAL GOVERNMENTS, AND NON-PROFIT ORGANIZATIONS Auditors § 41.525 Criteria for Federal program risk. (a) General. The auditor's determination should be based on an overall evaluation of the risk of noncompliance occurring, which could be material to the Federal program. The auditor shall...

Help

ERIC Educational Resources Information Center

Tollefson, Ann

2009-01-01

Planning to start or expand a K-8 critical language program? Looking for support in doing so? There "may" be help at the federal level for great ideas and strong programs. While there have been various pools of federal dollars available to support world language programs for a number of years, the federal government's interest in…

75 FR 32972 - Federal Employees Health Benefits Program; Medically Underserved Areas for 2011

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-10

... OFFICE OF PERSONNEL MANAGEMENT Federal Employees Health Benefits Program; Medically Underserved Areas for 2011 AGENCY: Office of Personnel Management. ACTION: Notice. SUMMARY: The U.S. Office of... Underserved Areas under the Federal Employees Health Benefits (FEHB) Program for calendar year 2011. This is...

76 FR 31998 - Federal Employees Health Benefits Program: Medically Underserved Areas for 2012

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-02

... OFFICE OF PERSONNEL MANAGEMENT Federal Employees Health Benefits Program: Medically Underserved Areas for 2012 AGENCY: U.S. Office of Personnel Management. ACTION: Notice. SUMMARY: The U.S. Office of... Underserved Areas under the Federal Employees Health Benefits (FEHB) Program for calendar year 2012. This is...

34 CFR 674.1 - Purpose and identification of common provisions.

Code of Federal Regulations, 2011 CFR

2011-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FEDERAL PERKINS LOAN PROGRAM General Provisions § 674.1 Purpose and identification of common provisions. (a) The Federal Perkins Loan Program provides low-interest... educational costs. (b)(1) The Federal Perkins Loan Program, authorized by title IV-E of the Higher Education...

34 CFR 674.1 - Purpose and identification of common provisions.

Code of Federal Regulations, 2010 CFR

2010-07-01

... POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FEDERAL PERKINS LOAN PROGRAM General Provisions § 674.1 Purpose and identification of common provisions. (a) The Federal Perkins Loan Program provides low-interest... educational costs. (b)(1) The Federal Perkins Loan Program, authorized by title IV-E of the Higher Education...

Federal Research and Demonstration Programs Benefiting the Disadvantaged and Handicapped.

ERIC Educational Resources Information Center

1968

Purpose, authority, applicant requirements, project deadlines, federal funding matching stipulations, program focus, 1968 priorities, and sources for information and guidelines are given for 23 programs and projects administered by the Federal Government. Included are those of the Office of Education, Social and Rehabilitation Service, and Public…

75 FR 22809 - Mandatory Guidelines for Federal Workplace Drug Testing Programs

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-30

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Mandatory Guidelines for Federal Workplace Drug Testing... for Federal Workplace Drug Testing Programs (Mandatory Guidelines) from May 1, 2010, to October 1... drug testing programs as soon as possible that they will not be expected to implement the revisions to...

78 FR 69612 - Negotiated Rulemaking Committee, Negotiator Nominations and Schedule of Committee Meetings-Title...

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2013-11-20

... Committee, Negotiator Nominations and Schedule of Committee Meetings--Title IV Federal Student Aid Programs... the Federal Student Aid programs authorized under title IV of the Higher Education Act of 1965, as amended (HEA) (title IV Federal Student Aid programs). The committee will include representatives of...

43 CFR 4.1366 - Burdens of proof.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., assignment, or sale of rights granted under a permit— (1) If the applicant is seeking review, OSMRE shall... Special Rules Applicable to Surface Coal Mining Hearings and Appeals Request for Review of Approval Or... Sale of Rights Granted Under Permit (federal Program; Federal Lands Program; Federal Program for Indian...

77 FR 56845 - Multi-Agency Informational Meeting Concerning Compliance With the Federal Select Agent Program...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-14

... Informational Meeting Concerning Compliance With the Federal Select Agent Program; Public Webcast AGENCY... with the Federal Select Agent Program. The purpose of this notice is to notify all interested parties... changes to the select agent regulations; occupational health, information and physical security; personnel...

5 CFR 792.216 - Are Federal employees with children who are enrolled in summer programs and part-time programs...

Code of Federal Regulations, 2012 CFR

2012-01-01

... are enrolled in summer programs and part-time programs eligible for the child care subsidy program... summer programs and part-time programs eligible for the child care subsidy program? Federal employees... enrolled in daytime summer programs and part-time programs such as before and after school programs are...

5 CFR 792.216 - Are Federal employees with children who are enrolled in summer programs and part-time programs...

Code of Federal Regulations, 2011 CFR

2011-01-01

... are enrolled in summer programs and part-time programs eligible for the child care subsidy program... summer programs and part-time programs eligible for the child care subsidy program? Federal employees... enrolled in daytime summer programs and part-time programs such as before and after school programs are...

5 CFR 792.216 - Are Federal employees with children who are enrolled in summer programs and part-time programs...

Code of Federal Regulations, 2010 CFR

2010-01-01

... are enrolled in summer programs and part-time programs eligible for the child care subsidy program... summer programs and part-time programs eligible for the child care subsidy program? Federal employees... enrolled in daytime summer programs and part-time programs such as before and after school programs are...

Federal funding for health security in FY2015.

PubMed

Boddie, Crystal; Sell, Tara Kirk; Watson, Matthew

2014-01-01

Previous articles in this series have provided funding information for federal civilian biodefense programs and programs focused on radiological and nuclear preparedness and consequence management. This year the authors have expanded the focus of the analysis to US federal funding for health security. This article provides proposed funding amounts for FY2015, estimated amounts for FY2014, and actual amounts for FY2010 through FY2013 in 5 domains critical to health security: biodefense programs, radiological and nuclear programs, chemical programs, pandemic influenza and emerging infectious disease programs, and multiple-hazard and preparedness programs.

Study of Federal technology transfer activities in areas of interest to NASA Office of Space and Terrestrial Applications

NASA Technical Reports Server (NTRS)

Madigan, J. A.; Earhart, R. W.

1978-01-01

Forty-three ongoing technology transfer programs in Federal agencies other than NASA were selected from over 200 current Federal technology transfer activities. Selection was made and specific technology transfer mechanisms utilized. Detailed information was obtained on the selected programs by reviewing published literature, and conducting telephone interviews with each program manager. Specific information collected on each program includes technology areas; user groups, mechanisms employed, duration of program, and level of effort. Twenty-four distinct mechanisms are currently employed in Federal technology transfer activities totaling $260 million per year. Typical applications of each mechanism were reviewed, and caveats on evaluating program effectiveness were discussed. A review of recent federally funded research in technology transfer to state and local governments was made utilizing the Smithsonian Science Information Exchange, and abstracts of interest to NASA were selected for further reference.

Does the Federal Work-Study Program Really Work--And for Whom? Research Brief

ERIC Educational Resources Information Center

Scott-Clayton, Judith; Zhou, Rachel Yang

2017-01-01

The Federal Work-Study (FWS) program is one of the oldest federal programs intended to promote college access and persistence for low-income students. Since 1964, the program has provided approximately $1 billion annually to cover up to 75 percent of the wages of student employees, who typically work on campus for 10 to 15 hours per week. The FWS…

78 FR 2675 - Mandatory Guidelines for Federal Workplace Drug Testing Programs

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-14

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Mandatory Guidelines for Federal Workplace Drug Testing... Federal Workplace Drug Testing Programs (Mandatory Guidelines), effective on October 1, 2010, addresses... performing a review of Federal employee drug testing results that seek approval by the Secretary must submit...

Reforming and Consolidating Federal Job Training Programs. Hearing on Examining Proposals To Reform and Consolidate Federal Job Training Programs, before the Committee on Labor and Human Resources. United States Senate, One Hundred Third Congress, Second Session.

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. Senate Committee on Labor and Human Resources.

This hearing is a continuation of a bipartisan effort to consolidate, reform, and revitalize federally funded job training programs. Testimony includes statements of U.S. senators and individuals representing the following: National Association of State Job Training Coordinating Council and Human Resource Investment Council; American Federation of…

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2012 CFR

2012-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2013 CFR

2013-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2014 CFR

2014-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

36 CFR 71.15 - The Golden Eagle Insignia.

Code of Federal Regulations, 2011 CFR

2011-07-01

... contribute to the public's information and education about the Federal recreation fee program. (5) The term... other Federal agencies. (6) The Golden Eagle program refers to the Federal outdoor recreation fee...

78 FR 53433 - Annual Notice of Interest Rates of Federal Student Loans Made Under the Federal Family Education...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-29

... Notice of Interest Rates of Federal Student Loans Made Under the Federal Family Education Loan Program... Federal Student Aid announces the interest rates for the period July 1, 2013, through June 30, 2014, for..., 2010. The Chief Operating Officer takes this action to give notice of FFEL Program loan interest rates...

5 CFR 890.1301 - Purpose.

Code of Federal Regulations, 2013 CFR

2013-01-01

... EMPLOYEES HEALTH BENEFITS PROGRAM Department of Defense Federal Employees Health Benefits Program... enroll in health benefit plans offered under the Federal Employees Health Benefits (FEHB) Program in...

5 CFR 890.1301 - Purpose.

Code of Federal Regulations, 2011 CFR

2011-01-01

... EMPLOYEES HEALTH BENEFITS PROGRAM Department of Defense Federal Employees Health Benefits Program... enroll in health benefit plans offered under the Federal Employees Health Benefits (FEHB) Program in...

5 CFR 890.1301 - Purpose.

Code of Federal Regulations, 2014 CFR

2014-01-01

... EMPLOYEES HEALTH BENEFITS PROGRAM Department of Defense Federal Employees Health Benefits Program... enroll in health benefit plans offered under the Federal Employees Health Benefits (FEHB) Program in...

5 CFR 890.1301 - Purpose.

Code of Federal Regulations, 2012 CFR

2012-01-01

... EMPLOYEES HEALTH BENEFITS PROGRAM Department of Defense Federal Employees Health Benefits Program... enroll in health benefit plans offered under the Federal Employees Health Benefits (FEHB) Program in...

Making of federal coal policy: lessons for public lands management from a failed program, an essay and review

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tarlock, A.D.

1985-04-01

The federal coal leasing program implemented in the 1970s has not achieved its stated objectives and has resulted in a misallocation of resources. As of 1984, federal coal still had not played a major role in meeting either total national energy supply demands or even in meeting total coal supply demands. Federal coal accounted for only 12% of the total US production in 1982. The author notes the program has failed to coordinate coal development with other government objectives such as environmental protection; the program, moreover, has not achieved a satisfactory return to the federal treasury. He feels all threemore » branches of government must share the blame for failure of the program. The Department of the Interior's (USDI) attempts to develop a national coal leasing program failed in part because USDI could not agree on a set of workable and consistent conceptual underpinnings for the program. Administrative attempts also failed because of well-meaning but inept judicial intervention and political and economic events beyond the Department's control. Congress must also bear a good part of the blame. Congress never settled on a consistent coal use policy, and finally, after 1982-83, Congress lost faith in the USDI and attempted to legislate its own federal policy. The failure of the federal government to induce the greater use of federal coal is especially striking because ownership would seem to predict greater control over the use of the resources. 76 references.« less

78 FR 11857 - Agency Information Collection Activities; Comment Request; William D. Ford Federal Direct Loan...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-20

...; Comment Request; William D. Ford Federal Direct Loan Program (DL) Regulations AGENCY: Department of... in response to this notice will be considered public records. Title of Collection: William D. Ford.... Abstract: The William D. Ford Federal Direct Loan Program regulations cover areas of program administration...

41 CFR 101-6.2103 - What programs and activities of GSA are subject to these regulations?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true What programs and... Property Management Federal Property Management Regulations System FEDERAL PROPERTY MANAGEMENT REGULATIONS...? The Administrator publishes in the Federal Register a list of GSA's programs and activities that are...

Federal Programs Supporting Educational Change, Vol. 5: Executive Summary.

ERIC Educational Resources Information Center

Berman, Paul; And Others

Under the sponsorship of the U. S. Office of Education, Rand is conducting a several-year study of federally funded programs designed to introduce and spread innovative practices in public schools. These change agent programs normally offer temporary federal funding to school districts as "seed money" or "risk capital." If an…

5 CFR 870.510 - Continuation of eligibility for former Federal employees of the Civilian Marksmanship Program.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 5 Administrative Personnel 2 2013-01-01 2013-01-01 false Continuation of eligibility for former Federal employees of the Civilian Marksmanship Program. 870.510 Section 870.510 Administrative Personnel... LIFE INSURANCE PROGRAM Coverage § 870.510 Continuation of eligibility for former Federal employees of...

5 CFR 870.510 - Continuation of eligibility for former Federal employees of the Civilian Marksmanship Program.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Continuation of eligibility for former Federal employees of the Civilian Marksmanship Program. 870.510 Section 870.510 Administrative Personnel... LIFE INSURANCE PROGRAM Coverage § 870.510 Continuation of eligibility for former Federal employees of...

3 CFR - Federal Support for the Randolph-Sheppard Vending Facility Program

Code of Federal Regulations, 2013 CFR

2013-01-01

... 3 The President 1 2013-01-01 2013-01-01 false Federal Support for the Randolph-Sheppard Vending Facility Program Presidential Documents Other Presidential Documents Memorandum of January 20, 2012 Federal Support for the Randolph-Sheppard Vending Facility Program Memorandum for the Heads of Executive Departments and Agencies Thousands of American...

75 FR 69452 - Solicitation of Information and Recommendations for Supplementing the Guidance Provided in the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-12

... Effect of Exclusion from Participation in Federal Health Care Programs AGENCY: Office of Inspector... from Participation in Federal Health Care Programs (64 FR 52791; September 30, 1999) and solicits input... Federal Health Care Programs. Please assist us by referencing the file code OIG-115-N. Inspection of...

Evaluating Federal Social Programs: Finding out What Works and What Does Not

ERIC Educational Resources Information Center

Muhlhausen, David B.

2012-01-01

Federal social programs are rarely evaluated to determine whether they are actually accomplishing their intended purposes. As part of its obligation to spend taxpayers' dollars wisely, Congress should mandate that experimental evaluations of every federal social program be conducted. The evaluations should be large-scale, multisite studies to…

77 FR 14482 - Petroleum Reduction and Alternative Fuel Consumption Requirements for Federal Fleets

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-12

... agencies to use this methodology to determine fleet inventory targets and to prepare fleet management plans.... Department of Energy, Office of Energy Efficiency and Renewable Energy, Federal Energy Management Program (EE... DOE receives will be made available on the Federal Energy Management Program's Federal Fleet...

75 FR 58329 - Federal Travel Regulation (FTR); Relocation Expenses Test Programs

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-24

...; Docket 2010-0016; Sequence 1] RIN 3090-ZA01 Federal Travel Regulation (FTR); Relocation Expenses Test... extended the authority for relocation expenses test programs for Federal employees, made by the passage of..., permits the Administrator of General Services to authorize Federal agencies to test new and innovative...

34 CFR 668.24 - Record retention and examinations.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Participants in the Federal Perkins Loan Program shall follow procedures established in 34 CFR 674.19 for... media format; and (v) Participants in the Federal Perkins Loan Program shall follow procedures... administration of the Federal Perkins Loan, FWS, FSEOG, Federal Pell Grant, ACG, National SMART Grant, or TEACH...

77 FR 71584 - Agency Information Collection Activities; Comment Request; Loan Cancellation in the Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-03

...; Comment Request; Loan Cancellation in the Federal Perkins Loan Program AGENCY: Department of Education (ED... of Collection: Loan Cancellation in the Federal Perkins Loan Program. OMB Control Number: 1845-0100... these regulations are necessary to determine Federal Perkins Loan borrower's eligibility to receive...

An Introduction to the Federal Information Exchange System.

ERIC Educational Resources Information Center

De Santi, Vincent; Scheele, D. Sam

This publication offers a basic introduction to the Federal Information Exchange System (FIXS), a set of procedures for periodically collecting, processing, and publishing data on federal and state expenditures for each program listed in the "Catalog of Federal Assistance Programs." FIXS is designed for use in conjunction with the…

Federal Funding of Postsecondary Education in the 13 Western States.

ERIC Educational Resources Information Center

Weldon, Kent; Makowski, David

This summary report on the distribution of federal postsecondary education funds in the Western states is presented by the Western Interstate Commission for Higher Education. The basic concerns addressed in the study are: What major federal assistance programs affect postsecondary education? How should federal program assistance to states be…

77 FR 39717 - Assistance to Firefighters Grant Program

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

... DEPARTMENT OF HOMELAND SECURITY Federal Emergency Management Agency [Docket ID FEMA-2012-0022] Assistance to Firefighters Grant Program AGENCY: Federal Emergency Management Agency, DHS. ACTION: Notice; correction. On June 22, 2012, the Federal Emergency Management Agency (FEMA) published a notice in the Federal Register at 77 FR 37687 notifying...

Federal Compliance Works against Education Policy Goals. Education Outlook. No. 6

ERIC Educational Resources Information Center

Junge, Melissa; Krvaric, Sheara

2011-01-01

While the federal government spends billions of dollars every year on federal education programs, federal policymakers and education advocates often lament that these programs do not achieve their intended results--specifically, increasing student academic achievement. To address this problem, policymakers and advocates typically debate the merits…

77 FR 42085 - Federal Perkins Loan Program, Federal Family Education Loan Program, and William D. Ford Federal...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-17

... cohorts. Chart 1--Summary of the Proposed Regulations Issue and key features Benefits Cost/transfers... focus on issues related to streamlining institutional reporting requirements and proposed regulations... committee (the ``Loans Committee'') would address Federal student loan issues. The regulations considered by...

34 CFR 668.42 - Financial assistance information.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Loan Program, the William D. Ford Federal Direct Student Loan Program, and the Federal Perkins Loan... institution provides and collects as required by 34 CFR 674.42 for borrowers under the Federal Perkins Loan...

The Geological Survey sediment program in California

USGS Publications Warehouse

Brown, E.

1957-01-01

The activities of the Water Resources Division of the U.S. Geological Survey in the State of California arise from the responsibility place on this agency by Congress for the determination and appraisal of the nation's water resources. The stream-sediment programs of the division are designed to carry out this broad responsibility and include systematic measurement of the sediment load carried by streams, studies of course and movement of fluvial sediment, and research on the mechanics of fluvial-sediment movement. In order to effectively consummate these programs over the Nation, finds are appropriated by Congress and earmarked in part from what is termed the federal program and in part for the cooperative program to match on a 50-50 basis, offerings at the State or local level. The federal stream-sediment program is comprised of investigations in which the federal steam-sediment program is comprised of investigations in which the federal or national interest is predominant and, accordingly, the costs are borne entirely by the federal government. It is expected that come all-federal finds will be allocated in fiscal year 1958 to projects in California, but the amount us not as yet known.

48 CFR 519.7016 - Program review.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Program review. 519.7016 Section 519.7016 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS GSA Mentor-Protégé Program 519.7016 Program review. At the conclusion of...

Three Spokane Moms Save Their School Libraries

ERIC Educational Resources Information Center

Whelan, Debra Lau

2008-01-01

In this article, the author reports on how three women saved their school libraries and created an advocacy model for all. The dynamic trio: Lisa Layera Brunkan, the great researcher with exceptional people skills, Susan McBurney, the master organizer with a Ph.D. in linguistics, and Denette Hill, the numbers lady with fantastic business acumen,…

Memory as Discrimination: A Challenge to the Encoding-Retrieval Match Principle

ERIC Educational Resources Information Center

Poirier, Marie; Nairne, James S.; Morin, Caroline; Zimmermann, Friederike G. S.; Koutmeridou, Kyriaki; Fowler, James

2012-01-01

Four experiments contrasted the predictions of a general encoding-retrieval match hypothesis with those of a view claiming that the distinctiveness of the cue-target relationship is the causal factor in retrieval. In Experiments 1, 2, and 4 participants learned the relationships between 4 targets and trios of cues; in Experiment 3 there were 3…

A Recommendation for a New Internet-based Environment for Studying Literature

ERIC Educational Resources Information Center

Kartal, Erdogan; Arikan, Arda

2010-01-01

The effects of information and communication technologies, which are rapidly improving and spreading in the current age, can be seen in the field of training and education as well as in all other fields. Unlike previous technologies, the Internet, which is the concrete compound of those technologies, provides users with the trio of audio, text and…

Implementation of a Formative Assessment Model Incorporating Peer and Self-Assessment

ERIC Educational Resources Information Center

Orsmond, Paul; Merry, Stephen; Callaghan, Arthur

2004-01-01

This paper reports a study designed to enhance students' ability to implement assessment marking criteria and to develop the role of dialogue in student learning. Pairs or trios of undergraduate biology students were asked to complete a poster on the theme of histology using student/tutor-constructed marking criteria. The study showed: (1)…

Trio Student Support Services: A Comparative Study of African American Students at Three Texas Universities

ERIC Educational Resources Information Center

Powell, Hattie Marie

2013-01-01

Although access to postsecondary education for ethnic minorities has increased since desegregation in 1954, the college completion rates for these groups have not, particularly for African Americans. For this reason, it is important to continue to examine strategies that contribute to increases in completion rates for African Americans. The…

Creative Photography - Wildlife

NASA Image and Video Library

2016-03-25

Two juvenile roseate spoonbills are joined by a white ibis, foreground, as the trio wades in a shallow waterway at Merritt Island National Wildlife Refuge in Florida. NASA’s Kennedy Space Center shares boundaries with the refuge, which is home to more than 330 native and migratory bird species, along with 25 mammal, 117 fish, and 65 amphibian and reptile species.

Soyuz MS-06 Undocking_2018_58_623287

NASA Image and Video Library

2018-02-28

Expedition 54 Commander Alexander Misurkin of Roscosmos and Flight Engineers Mark Vande Hei and Joe Acaba of NASA bid farewell to their colleagues on the complex and undocked their Soyuz MS-06 spacecraft from the Poisk Module on the International Space Station. The trio spent almost six months in space conducting research and operational work in support of the station.

Generalist Pre-Service Teacher Education, Self-Efficacy and Arts Education: An Impossible Expectation?

ERIC Educational Resources Information Center

Collins, Anita

2016-01-01

Generalist teacher educators in Australia are struggling with an impossible expectation in the area of arts education. This is due to a cascading trio of systemic issues. Firstly generalist teachers are entering their teacher education courses with variable and often minimal personal arts training. Secondly they are ill supported to improve their…

The Impact of Significant Federal Student Loan Policy Changes and the Role of States in Student Credit.

ERIC Educational Resources Information Center

Wennerdahl, Carol; Boyd, Joseph D.

This monograph reviews the role of the states in federal student loan programs since the inception of the Guaranteed Student Loan Program under Title IV, Part B of the Higher Education Act of 1965. Part I examines the program during its first 15 years when the program was experiencing its most turbulent times. State and federal roles were fluid…

Promoting Educational Opportunity: The Pell Grant Program at Community Colleges. AACC Policy Brief 2011-03PBL

ERIC Educational Resources Information Center

Baime, David S.; Mullin, Christopher M.

2011-01-01

The Federal Pell Grant Program serves as the foundation of the Federal Student Aid system, with more than 9.5 million students relying on the program to provide access to higher education in 2010-2011. It is one of the rare large federal domestic programs that has almost entirely avoided suggestions that it is ineffective or that it plays a role…

Federal Geothermal Research Program Update - Fiscal Year 2001

DOE Office of Scientific and Technical Information (OSTI.GOV)

Laney, P.T.

2002-08-31

This Federal Geothermal Program Research Update reviews the specific objectives, status, and accomplishments of DOE's Geothermal Program for Federal Fiscal Year (FY) 2001. The information contained in this Research Update illustrates how the mission and goals of the Office of Geothermal Technologies are reflected in each R&D activity. The Geothermal Program, from its guiding principles to the most detailed research activities, is focused on expanding the use of geothermal energy.

Federal Family Education Loan Program: Increased Department of Education Oversight of Lender and School Activities Needed to Help Ensure Program Compliance. Report to Congressional Requesters. GAO-07-750

ERIC Educational Resources Information Center

Scott, George A.

2007-01-01

Concerns have been raised about the Department of Education's (Education) role in overseeing the lenders and schools that participate in the largest of the federal government's student loan programs, the Federal Family Education Loan Program (FFELP). GAO was asked to analyze Education's use of its oversight, guidance, and enforcement authorities…

An Historical Analysis and Comparison of the Military Retirement System and the Federal Employee Retirement system

DTIC Science & Technology

1998-06-01

retirement programs in existence. This thesis concentrates on the Civil Service Retirement System (CSRS) and its successor the Federal Employee Retirement...federal employees at the beginning of fiscal year 1995. (Ref. 11, p. 2) The basic objective of the CSRS and FERS programs is to attract quality...and FERS both provide pensions for retired federal employees , the programs are designed differently. CSRS was established in 1920 and predates the

Nurse-midwives in federally funded health centers: understanding federal program requirements and benefits.

PubMed

Carter, Martha

2012-01-01

Midwives are working in federally funded health centers in increasing numbers. Health centers provide primary and preventive health care to almost 20 million people and are located in every US state and territory. While health centers serve the entire community, they also serve as a safety net for low-income and uninsured individuals. In 2010, 93% of health center patients had incomes below 200% of the Federal Poverty Guidelines, and 38% were uninsured. Health centers, including community health centers, migrant health centers, health care for the homeless programs, and public housing primary care programs, receive grant funding and enjoy other benefits due to status as federal grantees and designation as federally qualified health centers. Clinicians working in health centers are also eligible for financial and professional benefits because of their willingness to serve vulnerable populations and work in underserved areas. Midwives, midwifery students, and faculty working in, or interacting with, health centers need to be aware of the regulations that health centers must comply with in order to qualify for and maintain federal funding. This article provides an overview of health center regulations and policies affecting midwives, including health center program requirements, scope of project policy, provider credentialing and privileging, Federal Tort Claims Act malpractice coverage, the 340B Drug Pricing Program, and National Health Service Corps scholarship and loan repayment programs. © 2012 by the American College of Nurse-Midwives.