Sample records for fide centrin essential

  1. Sperm with fibrous sheath dysplasia and anomalies in head-neck junction: focus on centriole and centrin 1.

    PubMed

    Moretti, E; Pascarelli, N A; Belmonte, G; Renieri, T; Collodel, G

    2017-09-01

    Spermatozoa with a rare combination of two monomorphic sperm defects, dysplasia of the fibrous sheath (DFS) and alterations in head-mid-piece junction were analysed. The main focus was to explore the status of the centriole, a key organisation during fertilisation, using the centrin 1, a calcium-binding protein linked to this structure. The sperm quality was examined by light, scanning and transmission electron microscopy (SEM, TEM); immunocytochemistry was performed for tubulin, A-kinase anchor protein 4 (AKAP4) and centrin 1. Spermatozoa showed DFS defect associated with anomalies in head-tail attachment detected by SEM and TEM. Immunolocalisation of tubulin, AKAP4 and centrin 1 confirmed these alterations. Centrin 1 was visible in 67% of spermatozoa (in only 13% centrin localised in a normal position); in the majority of sperm centrin 1's location was altered, sometimes bent; often four spots, indicating the presence of two implantation fossae, were detected. At the centriolar level, immunoreactive fragments, frequently invading the entire short and thick tail, were observed. Centrin 1 is an essential component of the spermatozoa connecting piece and plays a role in centrosome dynamics during sperm morphogenesis and in zygotes and early embryos during spindle assembly. It is important to shed light on these rare conditions in order to better manage the patients during assisted reproductive technology. © 2016 Blackwell Verlag GmbH.

  2. Centrin diversity and basal body patterning across evolution: new insights from Paramecium

    PubMed Central

    Balavoine, Guillaume; Lemullois, Michel; Bouhouche, Khaled; Beisson, Janine; Koll, France

    2017-01-01

    ABSTRACT First discovered in unicellular eukaryotes, centrins play crucial roles in basal body duplication and anchoring mechanisms. While the evolutionary status of the founding members of the family, Centrin2/Vfl2 and Centrin3/cdc31 has long been investigated, the evolutionary origin of other members of the family has received less attention. Using a phylogeny of ciliate centrins, we identify two other centrin families, the ciliary centrins and the centrins present in the contractile filaments (ICL centrins). In this paper, we carry on the functional analysis of still not well-known centrins, the ICL1e subfamily identified in Paramecium, and show their requirement for correct basal body anchoring through interactions with Centrin2 and Centrin3. Using Paramecium as well as a eukaryote-wide sampling of centrins from completely sequenced genomes, we revisited the evolutionary story of centrins. Their phylogeny shows that the centrins associated with the ciliate contractile filaments are widespread in eukaryotic lineages and could be as ancient as Centrin2 and Centrin3. PMID:28432105

  3. Calmyonemin: a 23 kDa analogue of algal centrin occurring in contractile myonemes of Eudiplodinium maggii (ciliate).

    PubMed

    David, C; Viguès, B

    1994-01-01

    Myonemes are bundles of thin filaments (3-6 nm in diameter) which mediate calcium-induced contraction of the whole or only parts of the cell body in a number of protists. In Eudiplodinium maggii, a rumen ciliate which lacks a uniform ciliation of the cell body, myonemes converge toward the bases of apical ciliary zones that can be retracted under stress conditions, entailing immobilization of the cell. An mAB (A69) has been produced that identifies a calcium-binding protein by immunoblot, immunoprecipitation experiments and specifically labels the myonemes in immunoelectron microscopy. Solubility properties, apparent molecular weight (23 kDa) and isoelectric point (4.9) of the myonemal protein, are similar to the values reported for the calcium-modulated contractile protein centrin. Western-blot analysis indicates that the 23 kDa protein cross-reacts antigenically with anti-centrin antibodies. In addition, the 23 kDa protein displays calcium-induced changes in both electrophoretic and chromatographic behaviour, and contains calcium-binding domains that conform to the EF-hand structure, as known for centrin. Based on these observations, we conclude that a calcium-binding protein with major similarities to centrin occurs in the myonemes of E. maggii. We postulate that this protein plays an essential role in myoneme-mediated retraction of the ciliature.

  4. hPOC5 is a centrin-binding protein required for assembly of full-length centrioles.

    PubMed

    Azimzadeh, Juliette; Hergert, Polla; Delouvée, Annie; Euteneuer, Ursula; Formstecher, Etienne; Khodjakov, Alexey; Bornens, Michel

    2009-04-06

    Centrin has been shown to be involved in centrosome biogenesis in a variety of eukaryotes. In this study, we characterize hPOC5, a conserved centrin-binding protein that contains Sfi1p-like repeats. hPOC5 is localized, like centrin, in the distal portion of human centrioles. hPOC5 recruitment to procentrioles occurs during G2/M, a process that continues up to the full maturation of the centriole during the next cell cycle and is correlated with hyperphosphorylation of the protein. In the absence of hPOC5, RPE1 cells arrest in G1 phase, whereas HeLa cells show an extended S phase followed by cell death. We show that hPOC5 is not required for the initiation of procentriole assembly but is essential for building the distal half of centrioles. Interestingly, the hPOC5 family reveals an evolutionary divergence between vertebrates and organisms like Drosophila melanogaster or Caenorhabditis elegans, in which the loss of hPOC5 may correlate with the conspicuous differences in centriolar structure.

  5. The Structure of the Human Centrin 2-Xeroderma Pigmentosum Group C Protein Complex

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Thompson,J.; Ryan, Z.; Salisbury, J.

    2006-01-01

    Human centrin-2 plays a key role in centrosome function and stimulates nucleotide excision repair by binding to the xeroderma pigmentosum group C protein. To determine the structure of human centrin-2 and to develop an understanding of molecular interactions between centrin and xeroderma pigmentosum group C protein, we characterized the crystal structure of calcium-loaded full-length centrin-2 complexed with a xeroderma pigmentosum group C peptide. Our structure shows that the carboxyl-terminal domain of centrin-2 binds this peptide and two calcium atoms, whereas the amino-terminal lobe is in a closed conformation positioned distantly by an ordered {alpha}-helical linker. A stretch of the amino-terminalmore » domain unique to centrins appears disordered. Two xeroderma pigmentosum group C peptides both bound to centrin-2 also interact to form an {alpha}-helical coiled-coil. The interface between centrin-2 and each peptide is predominantly nonpolar, and key hydrophobic residues of XPC have been identified that lead us to propose a novel binding motif for centrin.« less

  6. Molecular cloning and evolutionary analysis of the calcium-modulated contractile protein, centrin, in green algae and land plants.

    PubMed

    Bhattacharya, D; Steinkötter, J; Melkonian, M

    1993-12-01

    Centrin (= caltractin) is a ubiquitous, cytoskeletal protein which is a member of the EF-hand superfamily of calcium-binding proteins. A centrin-coding cDNA was isolated and characterized from the prasinophyte green alga Scherffelia dubia. Centrin PCR amplification primers were used to isolate partial, homologous cDNA sequences from the green algae Tetraselmis striata and Spermatozopsis similis. Annealing analyses suggested that centrin is a single-copy-coding region in T. striata and S. similis and other green algae studied. Centrin-coding regions from S. dubia, S. similis and T. striata encode four colinear EF-hand domains which putatively bind calcium. Phylogenetic analyses, including homologous sequences from Chlamydomonas reinhardtii and the land plant Atriplex nummularia, demonstrate that the domains of centrins are congruent and arose from the two-fold duplication of an ancestral EF hand with Domains 1+3 and Domains 2+4 clustering. The domains of centrins are also congruent with those of calmodulins demonstrating that, like calmodulin, centrin is an ancient protein which arose within the ancestor of all eukaryotes via gene duplication. Phylogenetic relationships inferred from centrin-coding region comparisons mirror results of small subunit ribosomal RNA sequence analyses suggesting that centrin-coding regions are useful evolutionary markers within the green algae.

  7. Centrins in unicellular organisms: functional diversity and specialization.

    PubMed

    Zhang, Yu; He, Cynthia Y

    2012-07-01

    Centrins (also known as caltractins) are conserved, EF hand-containing proteins ubiquitously found in eukaryotes. Similar to calmodulins, the calcium-binding EF hands in centrins fold into two structurally similar domains separated by an alpha-helical linker region, shaping like a dumbbell. The small size (15-22 kDa) and domain organization of centrins and their functional diversity/specialization make them an ideal system to study protein structure-function relationship. Here, we review the work on centrins with a focus on their structures and functions characterized in unicellular organisms.

  8. Apparent diffusive motion of centrin foci in living cells: implications for diffusion-based motion in centriole duplication

    NASA Astrophysics Data System (ADS)

    Rafelski, Susanne M.; Keller, Lani C.; Alberts, Jonathan B.; Marshall, Wallace F.

    2011-04-01

    The degree to which diffusion contributes to positioning cellular structures is an open question. Here we investigate the question of whether diffusive motion of centrin granules would allow them to interact with the mother centriole. The role of centrin granules in centriole duplication remains unclear, but some proposed functions of these granules, for example, in providing pre-assembled centriole subunits, or by acting as unstable 'pre-centrioles' that need to be captured by the mother centriole (La Terra et al 2005 J. Cell Biol. 168 713-22), require the centrin foci to reach the mother. To test whether diffusive motion could permit such interactions in the necessary time scale, we measured the motion of centrin-containing foci in living human U2OS cells. We found that these centrin foci display apparently diffusive undirected motion. Using the apparent diffusion constant obtained from these measurements, we calculated the time scale required for diffusion to capture by the mother centrioles and found that it would greatly exceed the time available in the cell cycle. We conclude that mechanisms invoking centrin foci capture by the mother, whether as a pre-centriole or as a source of components to support later assembly, would require a form of directed motility of centrin foci that has not yet been observed.

  9. Centrin protein and genes in Trichomonas vaginalis and close relatives.

    PubMed

    Brugerolle, G; Bricheux, G; Coffe, G

    2000-01-01

    Anti-centrin monoclonal antibodies 20H5 and 11B2 produced against Clamydomononas centrin decorated the group of basal bodies as well as very closely attached structures in all trichomonads studied and in the devescovinids Foaina and Devescovina. Moreover, these antibodies decorated the undulating membrane in Trichomonas vaginalis, Trichomitus batrachorum, and Tritrichomonas foetus, and the cresta in Foaina. Centrin was not demonstrated in the dividing spindle and paradesmosis. Immunogold labeling, both in pre- and post-embedding, confirmed that centrin is associated with the basal body cylinder and is a component of the nine anchoring arms between the terminal plate of flagellar bases and the plasma-membrane. Centrin is also associated with the hook-shaped fibers attached to basal bodies (F1, F3), the X-fiber, and along sigmoid fibers (F2) at the pelta-axostyle junction, which is the microtubule organizing center for pelta-axostyle microtubules. There was no labeling on the striated costa and parabasal fibers nor on microtubular pelta-axostyle, but the fibrous structure inside the undulating membrane was labeled in T. vaginalis. Two proteins of 22-20 kDa corresponding to the centrin molecular mass were recognized by immunoblotting using these antibodies in the three trichomonad species examined. By screening a T. vaginalis cDNA library with 20H5 antibody, two genes encoding identical protein sequences were found. The sequence comprises the 4 typical EF-hand Ca++-binding domains present in every known centrin. Trichomonad centrin is closer to the green algal cluster (70% identity) than to the yeast Cdc31 cluster (55% identity) or the Alveolata cluster (46% identity).

  10. A chirality change in XPC- and Sfi1-derived peptides affects their affinity for centrin.

    PubMed

    Grecu, Dora; Irudayaraj, Victor Paul Raj; Martinez-Sanz, Juan; Mallet, Jean-Maurice; Assairi, Liliane

    2016-04-01

    The Ca(2+)-binding protein centrin binds to a hydrophobic motif (W(1)xxL(4)xxxL(8)) included in the sequence of several cellular targets: XPC (xeroderma pigmentosum group C protein), Sfi1 (suppressor of fermentation-induced loss of stress resistance protein1), and Sac3 [the central component of the transcription and mRNA export (TREX-2) complex]. However, centrin binding occurs in a reversed orientation (L(8)xxxL(4)xxW(1)) for Sfi1 and Sac3 compared with XPC. Because D-peptides have been investigated for future therapeutic use, we analyzed their centrin-binding properties. Their affinity for centrin was measured using isothermal titration calorimetry. The chirality change in the target-derived peptides affected their ability to bind centrin in a specific manner depending on the sequence orientation of the centrin-binding motif. In contrast to L-XPC-P10, D-XPC-P10 bound C-HsCen1 in a Ca(2+)-dependent manner and to a lesser extent. D-XPC-P10 exhibited a reduced affinity for C-HsCen1 (Ka=0.064 × 10(6) M(-1)) by a factor of 2000 compared with L-XPC-P10 (Ka=132 × 10(6) M(-1)). D-peptides have a lower affinity than L-peptides for centrin, and the strength of this affinity depends on the sequence orientation of the target-derived peptides. The residual affinity observed for D-XPC suggests that the use of d-peptides represents a promising strategy for inhibiting centrin binding to its targets. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. Hydrogen/Deuterium Exchange Reflects Binding of Human Centrin 2 to Ca2+ and Xeroderma Pigmentosum Group C Peptide: An Example of EX1 Kinetics

    PubMed Central

    Sperry, Justin B.; Ryan, Zachary C.; Kumar, Rajiv; Gross, Michael L.

    2012-01-01

    Xeroderma pigmentosum (XP) is a genetic disease affecting 1 in 10,000-100,000 and predisposes people to early-age skin cancer, a disease that is increasing. Those with XP have decreased ability to repair UV-induced DNA damage, leading to increased susceptibility of cancerous non-melanomas and melanomas. A vital, heterotrimeric protein complex is linked to the nucleotide excision repair pathway for the damaged DNA. The complex consists of XPC protein, human centrin 2, and RAD23B. One of the members, human centrin 2, is a ubiquitous, acidic, Ca2+-binding protein belonging to the calmodulin superfamily. The XPC protein contains a sequence motif specific for binding to human centrin 2. We report here the Ca2+-binding properties of human centrin 2 and its interaction with the XPC peptide motif. We utilized a region-specific H/D exchange protocol to localize the interaction of the XPC peptide with the C-terminal domain of centrin, the binding of which is different than that of calmodulin complexes. The binding dynamics of human centrin 2 to the XPC peptide in the absence and presence of Ca2+ are revealed by the observation of EX1 H/D exchange regime, indicating that a locally unfolded population exists in solution and undergoes fast H/D exchange. PMID:23439742

  12. Human TREX2 components PCID2 and centrin 2, but not ENY2, have distinct functions in protein export and co-localize to the centrosome.

    PubMed

    Cunningham, Corey N; Schmidt, Casey A; Schramm, Nathaniel J; Gaylord, Michelle R; Resendes, Karen K

    2014-01-15

    TREX-2 is a five protein complex, conserved from yeast to humans, involved in linking mRNA transcription and export. The centrin 2 subunit of TREX-2 is also a component of the centrosome and is additionally involved in a distinctly different process of nuclear protein export. While centrin 2 is a known multifunctional protein, the roles of other human TREX-2 complex proteins other than mRNA export are not known. In this study, we found that human TREX-2 member PCID2 but not ENY2 is involved in some of the same cellular processes as those of centrin 2 apart from the classical TREX-2 function. PCID2 is present at the centrosome in a subset of HeLa cells and this localization is centrin 2 dependent. Furthermore, the presence of PCID2 at the centrosome is prevalent throughout the cell cycle as determined by co-staining with cyclins E, A and B. PCID2 but not ENY2 is also involved in protein export. Surprisingly, siRNA knockdown of PCID2 delayed the rate of nuclear protein export, a mechanism distinct from the effects of centrin 2, which when knocked down inhibits export. Finally we showed that co-depletion of centrin 2 and PCID2 leads to blocking rather than delaying nuclear protein export, indicating the dominance of the centrin 2 phenotype. Together these results represent the first discovery of specific novel functions for PCID2 other than mRNA export and suggest that components of the TREX-2 complex serve alternative shared roles in the regulation of nuclear transport and cell cycle progression. © 2013 Published by Elsevier Inc.

  13. Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function

    PubMed Central

    Sawant, Dwitiya B.; Majumder, Shubhra; Perkins, Jennifer L.; Yang, Ching-Hui; Eyers, Patrick A.; Fisk, Harold A.

    2015-01-01

    Centrins are a family of small, calcium-binding proteins with diverse cellular functions that play an important role in centrosome biology. We previously identified centrin 2 and centrin 3 (Cetn2 and Cetn3) as substrates of the protein kinase Mps1. However, although Mps1 phosphorylation sites control the function of Cetn2 in centriole assembly and promote centriole overproduction, Cetn2 and Cetn3 are not functionally interchangeable, and we show here that Cetn3 is both a biochemical inhibitor of Mps1 catalytic activity and a biological inhibitor of centrosome duplication. In vitro, Cetn3 inhibits Mps1 autophosphorylation at Thr-676, a known site of T-loop autoactivation, and interferes with Mps1-dependent phosphorylation of Cetn2. The cellular overexpression of Cetn3 attenuates the incorporation of Cetn2 into centrioles and centrosome reduplication, whereas depletion of Cetn3 generates extra centrioles. Finally, overexpression of Cetn3 reduces Mps1 Thr-676 phosphorylation at centrosomes, and mimicking Mps1-dependent phosphorylation of Cetn2 bypasses the inhibitory effect of Cetn3, suggesting that the biological effects of Cetn3 are due to the inhibition of Mps1 function at centrosomes. PMID:26354417

  14. Centrin-like filaments in the cytopharyngeal apparatus of the ciliates Nassula and Furgasonia: evidence for a relationship with microtubular structures.

    PubMed

    Vigues, B; Blanchard, M P; Bouchard, P

    1999-01-01

    The cytopharyngeal apparatus in the Nassulinid ciliates Nassula and Furgasonia is a highly specialized microtubular/filamentous organelle designed for ingestion of organisms such as filamentous bacteria. From studies on living cells, it was previously shown that this organelle, also called "feeding basket," guides the filamentous bacteria and manipulates them to some extent during the early steps of ingestion. This results in a complex sequence of movements where the basket is successively dilated and constricted in its upper part. Whereas some of these movements (dilation) seem to be intrinsic to the microtubular components of the basket, others (constriction) are believed to be mediated by contractile filamentous structures [Tucker, 1968: J. Cell Sci. 3:493-514]. In this study, we have used antibodies raised against ciliate centrins to demonstrate these proteins by Western blot and immunocytochemical methods in Nassula and Furgasonia. In both ciliates, a 20-kDa centrin immunoanalog was localized in the upper (contractile) part of the cytopharyngeal apparatus. Immunoelectron microscopy revealed that cytopharyngeal centrin is engaged in filamentous material, forming a sphincter-like structure possibly involved in the movements of contraction. Interestingly, physical links were noted between filaments labeled for centrin and cytopharyngeal microtubules. The mechanistic implications of these findings are discussed.

  15. Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in Tetrahymena thermophila

    PubMed Central

    Stemm-Wolf, Alexander J.; Meehl, Janet B.; Winey, Mark

    2013-01-01

    Summary Directed fluid flow, which is achieved by the coordinated beating of motile cilia, is required for processes as diverse as cellular swimming, developmental patterning and mucus clearance. Cilia are nucleated, anchored and aligned at the plasma membrane by basal bodies, which are cylindrical microtubule-based structures with ninefold radial symmetry. In the unicellular ciliate Tetrahymena thermophila, two centrin family members associated with the basal body are important for both basal body organization and stabilization. We have identified a family of 13 proteins in Tetrahymena that contain centrin-binding repeats related to those identified in the Saccharomyces cerevisiae Sfi1 protein. We have named these proteins Sfr1–Sfr13 (for Sfi1-repeat). Nine of the Sfr proteins localize in unique polarized patterns surrounding the basal body, suggesting non-identical roles in basal body organization and association with basal body accessory structures. Furthermore, the Sfr proteins are found in distinct basal body populations in Tetrahymena cells, indicating that they are responsive to particular developmental programs. A complete genetic deletion of one of the family members, Sfr13, causes unstable basal bodies and defects in daughter basal body separation from the mother, phenotypes also observed with centrin disruption. It is likely that the other Sfr family members are involved in distinct centrin functions, providing specificity to the tasks that centrins perform at basal bodies. PMID:23426847

  16. Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.

    PubMed

    Sawant, Dwitiya B; Majumder, Shubhra; Perkins, Jennifer L; Yang, Ching-Hui; Eyers, Patrick A; Fisk, Harold A

    2015-11-01

    Centrins are a family of small, calcium-binding proteins with diverse cellular functions that play an important role in centrosome biology. We previously identified centrin 2 and centrin 3 (Cetn2 and Cetn3) as substrates of the protein kinase Mps1. However, although Mps1 phosphorylation sites control the function of Cetn2 in centriole assembly and promote centriole overproduction, Cetn2 and Cetn3 are not functionally interchangeable, and we show here that Cetn3 is both a biochemical inhibitor of Mps1 catalytic activity and a biological inhibitor of centrosome duplication. In vitro, Cetn3 inhibits Mps1 autophosphorylation at Thr-676, a known site of T-loop autoactivation, and interferes with Mps1-dependent phosphorylation of Cetn2. The cellular overexpression of Cetn3 attenuates the incorporation of Cetn2 into centrioles and centrosome reduplication, whereas depletion of Cetn3 generates extra centrioles. Finally, overexpression of Cetn3 reduces Mps1 Thr-676 phosphorylation at centrosomes, and mimicking Mps1-dependent phosphorylation of Cetn2 bypasses the inhibitory effect of Cetn3, suggesting that the biological effects of Cetn3 are due to the inhibition of Mps1 function at centrosomes. © 2015 Sawant et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  17. The rhizoplast of chrysomonads, a basal body-nucleus connector that polarises the dividing spindle.

    PubMed

    Brugerolle, G; Mignot, J-P

    2003-09-01

    An ultrastructure study of the rhizoplast in Synura petersenii, Mallomonas fastigiata, and M. insignis shows that it consists of 15-20 striated rootlets that form a claw or an incomplete cone over the nucleus. These rootlets course along one face of the nucleus between the nuclear membrane and the cis-face of the Golgi stack of cisternae. They converge and merge above the nucleus, forming a stub attached to the proximal section of the two basal bodies. These cross-striated rootlets are composed of closely packed longitudinal microfibrils. By immunofluorescence, the basal bodies and the rootlets forming the claw were decorated by the anti-centrin monoclonal antibody ICL19 raised against the Paramecium tetraurelia acidic centrin protein and by two antibodies raised against the striated parabasal and costal striated fibres of trichomonads. Only the anti-centrin monoclonal antibody 20H5 raised against Chlamydomonas reinhardtii centrin strongly labelled the 20-22 kDa protein bands from the extracted cytoskeleton of S. petersenii by immunoblotting. Electron micrographs of mitosis in S. petersenii cells revealed that the segregated pairs of basal bodies are linked by the striated rootlets of the rhizoplast to the poles of the mitotic spindle. The spindle microtubules arise perpendicularly from the striated rootlets of the basal body-nucleus connector forming the centrosome. In conclusion, in these cells there is a basal body-nucleus connector similar to that of C. reinhardtii and other chlorophytes. It contains centrin proteins, it is involved in the linkage of the basal bodies to the nucleus and is a component of the spindle pole body or centrosome in the dividing cell.

  18. Destruction of maternal centrioles during fertilization of the brown alga, Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae).

    PubMed

    Nagasato, Chikako; Motomura, Taizo

    2004-10-01

    In brown algal fertilization, a pair of centrioles is derived from the male gamete, irrespective of the sexual reproduction pattern, i.e., isogamy, anisogamy, or oogamy. In this study, the manner in which the maternal centriole structure is destroyed in early zygotes of the isogamous brown alga Scytosiphon lomentaria was examined by electron microscopy. At fertilization, the zygote had two pairs of centrioles (flagellar basal bodies) derived from motile male and female gametes, and there was no morphological difference between the two pairs. The flagellar basal plate and the axonemal microtubules were still connected with the distal end of centrioles. Ultrastructural observations showed that the integrity of maternal-derived centrioles began to degenerate even in the 1-h-old zygote. At that time, the cylinder of triplet microtubules of the maternal centrioles became shorter from the distal end, and a section passing through the centrioles indicated that a part of the nine triplets of microtubules changed into doublet or singlet microtubules by degeneration of B and/or C tubules. In 2-h-old zygote, there was no trace of maternal centrioles ultrastructurally, and only the paternal centrioles remained. Further, reduction of centrin accompanying destruction of the maternal centrioles was examined in immunofluorescence microscopy. Centrin localized at the paternal and the maternal centrioles had the same fluorescence intensity in the early zygotes. At 4-6 h after fertilization, two spots indicating centrin localization showed different fluorescence intensity. Later, the weaker spot disappeared completely. These results showed that there is a difference in time between the destruction of the centriolar cylinders and the reduction of centrin molecules around them. Copyright 2004 Wiley-Liss, Inc.

  19. Thermal perturbation correlation of calcium binding Human centrin 3 and its structural changes

    NASA Astrophysics Data System (ADS)

    Pastrana-Rios, Belinda

    2014-07-01

    Perturbation-correlation moving-window two-dimensional (PCMW2D) correlation spectroscopy was applied for the determination of the individual transition temperatures of different vibrational modes located within structural components of a calcium binding protein known as Human centrin 3. This crucial information served to understand the contribution individual calcium binding sites made towards the stability of the EF-hand and therefore the protein without the use of probes. We are convinced that the general application of PCMW2D correlation spectroscopy can be applied to the study of proteins in general to ascertain the differences in the stability of structural motifs within proteins and its relationship to the actual transition temperature of unfolding.

  20. Live Attenuated Leishmania donovani Centrin Gene-Deleted Parasites Induce IL-23-Dependent IL-17-Protective Immune Response against Visceral Leishmaniasis in a Murine Model.

    PubMed

    Banerjee, Antara; Bhattacharya, Parna; Dagur, Pradeep K; Karmakar, Subir; Ismail, Nevien; Joshi, Amritanshu B; Akue, Adovi D; KuKuruga, Mark; McCoy, John Philip; Dey, Ranadhir; Nakhasi, Hira L

    2018-01-01

    No vaccine exists against visceral leishmaniasis. To develop effective vaccines, we have previously reported protective role of live attenuated centrin gene-deleted Leishmania donovani ( LdCen -/- ) parasites through induction of Th1 type immune response in mice, hamsters, and dogs. In this study, we specifically explored the role of Th17 cells in LdCen -/- -induced host protection in mice. Our results showed that compared with wild-type L. donovani infection, LdCen -/- parasites induce significantly higher expression of Th17 differentiation cytokines in splenic dendritic cells. There was also induction of IL-17 and its promoting cytokines in total splenocytes and in both CD4 and CD8 T cells following immunization with LdCen -/- Upon challenge with wild-type parasites, IL-17 and its differentiating cytokines were significantly higher in LdCen -/- -immunized mice compared with nonimmunized mice that resulted in parasite control. Alongside IL-17 induction, we observed induction of IFN-γ-producing Th1 cells as reported earlier. However, Th17 cells are generated before Th1 cells. Neutralization of either IL-17 or IFN-γ abrogated LdCen -/- -induced host protection further confirming the essential role of Th17 along with Th1 cytokines in host protection. Treatment with recombinant IL-23, which is required for stabilization and maintenance of IL-17, heightened Th17, and Tc17 responses in immunized mice splenocytes. In contrast, Th17 response was absent in immunized IL-23R -/- mice that failed to induce protection upon virulent Leishmania challenge suggesting that IL-23 plays an essential role in IL-17-mediated protection by LdCen -/- parasites. This study unveiled the role of IL-23-dependent IL-17 induction in LdCen -/- parasite-induced immunity and subsequent protection against visceral leishmaniasis.

  1. N-terminal regions of Mps1 kinase determine functional bifurcation.

    PubMed

    Araki, Yasuhiro; Gombos, Linda; Migueleti, Suellen P S; Sivashanmugam, Lavanya; Antony, Claude; Schiebel, Elmar

    2010-04-05

    Mps1 is a conserved kinase that in budding yeast functions in duplication of the spindle pole body (SPB), spindle checkpoint activation, and kinetochore biorientation. The identity of Mps1 targets and the subdomains that convey specificity remain largely unexplored. Using a novel combination of systematic deletion analysis and chemical biology, we identified two regions within the N terminus of Mps1 that are essential for either SPB duplication or kinetochore biorientation. Suppression analysis of the MPS1 mutants defective in SPB duplication and biochemical enrichment of Mps1 identified the essential SPB components Spc29 and the yeast centrin Cdc31 as Mps1 targets in SPB duplication. Our data suggest that phosphorylation of Spc29 by Mps1 in G1/S recruits the Mps2-Bbp1 complex to the newly formed SPB to facilitate its insertion into the nuclear envelope. Mps1 phosphorylation of Cdc31 at the conserved T110 residue controls substrate binding to Kar1 protein. These findings explain the multiple SPB duplication defects of mps1 mutants on a molecular level.

  2. 26 CFR 54.4980H-0 - Table of contents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...) Applicable large employer member. (6) Applicable premium tax credit. (7) Bona fide volunteer. (8) Calendar... for certain employees. (27) Minimum essential coverage. (28) Minimum value. (29) Month. (30) New... measurement method applies, or vice versa. (2) Special rule for certain employees to whom minimum value...

  3. Defining New Treatment Approaches for KRAS-Mutant Lung Cancer

    DTIC Science & Technology

    2014-10-01

    mutant NSCLC , a challenge we must meet to make progress in this clinically challenging NSCLC subset. Mutant KRAS, like ALK or EGFR, is a bone fide NSCLC ...required for KRAS G12D-driven NSCLC . Specific Aim 1. To identify gene products specifically essential for KRAS-driven NSCLC , we will perform a shRNA...screen of thousands of mouse genes, looking for essentiality in multiple independent cell lines derived from two NSCLC GEMMs: one RAF- dependent and

  4. The sporulation of the green alga Ulva prolifera is controlled by changes in photosynthetic electron transport chain.

    PubMed

    Wang, Hui; Lin, Apeng; Gu, Wenhui; Huan, Li; Gao, Shan; Wang, Guangce

    2016-04-22

    Sporulation and spore release are essential phases of the life cycle in algae and land plants. Ulva prolifera, which is an ideal organism for studying sporulation and spore release, was used as the experimental material in the present study. The determination of photosynthetic parameters, combined with microscopic observation, treatment with photosynthetic inhibitors, limitation of carbon acquisition, and protein mass spectrometry, was employed in this experiment. Cycle electron transport (CEF) was found enhanced at the onset of sporangia formation. The inhibition effect of dibromothymoquinone (DBMIB) towards sporulation was always strong during the sporulation process whereas the inhibition effect of 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) was continuously declined accompanied with the progress of sporulation. The changes of photosynthesis resulted from the limitation of CO2 acquisition could stimulate sporulation onset. Quantitative protein analysis showed that enzymes involved in carbon fixation, including RUBISCO and pyruvate orthophosphate dikinase, declined during sporogenesis, while proteins involved in sporulation, including tubulin and centrin, increased. These results suggest that enhanced cyclic electron flow (CEF) and oxidation of the plastoquinone pool are essential for sporangia formation onset, and changes in photosynthetic electron transport chain have significant impacts on sporulation of the green algae.

  5. School Life That Matters: Building Circle of Courage Schools

    ERIC Educational Resources Information Center

    Van Bockern, Steve

    2014-01-01

    In the frenzy to spike test scores that track a standardized curriculum, the central question of what students actually need is ignored. What are the bona fide "essentials" in schools that enable children to learn and thrive? A school life that matters can be found in a school where children and adults are encouraged to pursue their…

  6. 19 CFR 10.152 - Bona-fide gifts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Bona-fide gifts. 10.152 Section 10.152 Customs... and Bona Fide Gifts § 10.152 Bona-fide gifts. Subject to the conditions in § 10.153 of this part, the port director shall pass free of duty and tax any article sent as a bona-fide gift from a person in a...

  7. Mutuality and solidarity: assessing risks and sharing losses.

    PubMed Central

    Wilkie, D

    1997-01-01

    Mutuality is the principle of private, commercial insurance; individuals enter the pool for sharing losses, and pay according to the best estimate of the risk they bring with them. Solidarity is the sharing of losses with payment according to some other scheme; this is the principle of state social insurance; essential features of solidarity are comprehensiveness and compulsion. Private insurance is subject to the uberrima fides principle, or utmost good faith; each side declares all it knows about the risk. The Disability Discrimination Act requires insurers to justify disability discrimination on the basis of relevant information, acturial, statistical or medical, on which it is reasonable to rely. It could be very damaging to private insurance to abandon uberrima fides. However, although some genetic information is clearly useful to underwriters, other information may be so general as to be of little use. The way in which mortality rates are assessed is also explained. PMID:9304668

  8. 78 FR 25181 - Revision of Regulations Defining Bona Fide Cotton Spot Markets

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-30

    ...-AD26 Revision of Regulations Defining Bona Fide Cotton Spot Markets AGENCY: Agricultural Marketing... regulation that specifies which states compose bona fide cotton spot markets in order to assure consistency with the revised Cotton Research and Promotion Act. Updated bona fide spot market definitions allow for...

  9. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 4 2011-07-01 2011-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  10. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 4 2012-07-01 2012-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  11. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 4 2013-07-01 2013-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  12. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 4 2014-07-01 2014-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  13. 29 CFR 1604.2 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Sex as a bona fide occupational qualification. 1604.2... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.2 Sex as a bona fide occupational qualification. (a) The commission believes that the bona fide occupational qualification exception as to sex should be interpreted...

  14. 19 CFR 145.32 - Bona-fide gifts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Bona-fide gifts. 145.32 Section 145.32 Customs... (CONTINUED) MAIL IMPORTATIONS Special Classes of Merchandise § 145.32 Bona-fide gifts. The port director... as bona-fide gifts from persons in foreign countries to persons in the United States having an...

  15. Generator exchange is associated with an increased rate of Sprint Fidelis lead failure.

    PubMed

    Lovelock, Joshua D; Patel, Ayesha; Mengistu, Andenet; Hoskins, Michael; El-Chami, Mikhael; Lloyd, Michael S; Leon, Angel; DeLurgio, David; Langberg, Jonathan J

    2012-10-01

    The Medtronic Sprint Fidelis defibrillator lead is at an increased risk for failure and was recalled in October 2007. Approximately 268,000 leads were implanted, and more than 100,000 patients still have active Fidelis leads. A number of studies have examined the rate and clinical predictors of lead failure, but none has addressed the effect of an implantable cardioverter-defibrillator generator exchange on subsequent lead failure. Although the manufacturer asserts that "Sprint Fidelis performance after device change-out is similar to lead performance without device change-out," published data are lacking. To assess the effect of implantable cardioverter-defibrillator generator exchange on the rate of Fidelis lead failure. A chart review was conducted in patients who underwent implantation of a Fidelis lead. Patients with a functioning Fidelis lead at generator exchange were compared with controls with leads implanted for a comparable amount of time not undergoing ICD replacement. A total of 1366 patients received a Fidelis lead prior to the recall, of which 479 were still actively followed. Seventy-two patients with a functioning lead underwent generator exchange without lead replacement. Following generator replacement, 15 leads failed. Sixty percent of the Fidelis leads failed within 3 months. Generator exchange increased the rate of lead failure compared with matched controls (20.8% vs 2.54%; P < .001). Generator exchange is associated with a higher than expected rate of Fidelis lead failure, often within 3 months. The risk-benefit ratio of Fidelis lead replacement at the time of generator exchange may be greater than appreciated. Copyright © 2012 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.

  16. 42 CFR 433.54 - Bona fide donations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Bona fide donations. 433.54 Section 433.54 Public... Financial Participation § 433.54 Bona fide donations. (a) A bona fide donation means a provider-related donation, as defined in § 433.52, made to the State or unit of local government, that has no direct or...

  17. 42 CFR 433.54 - Bona fide donations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 4 2013-10-01 2013-10-01 false Bona fide donations. 433.54 Section 433.54 Public... Financial Participation § 433.54 Bona fide donations. (a) A bona fide donation means a provider-related donation, as defined in § 433.52, made to the State or unit of local government, that has no direct or...

  18. 42 CFR 433.54 - Bona fide donations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 4 2012-10-01 2012-10-01 false Bona fide donations. 433.54 Section 433.54 Public... Financial Participation § 433.54 Bona fide donations. (a) A bona fide donation means a provider-related donation, as defined in § 433.52, made to the State or unit of local government, that has no direct or...

  19. 42 CFR 433.54 - Bona fide donations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 4 2011-10-01 2011-10-01 false Bona fide donations. 433.54 Section 433.54 Public... Financial Participation § 433.54 Bona fide donations. (a) A bona fide donation means a provider-related donation, as defined in § 433.52, made to the State or unit of local government, that has no direct or...

  20. 17 CFR 240.11a1-3(T) - Bona fide hedge transactions in certain securities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Bona fide hedge transactions in certain securities. 240.11a1-3(T) Section 240.11a1-3(T) Commodity and Securities Exchanges... (rule 11a-1) § 240.11a1-3(T) Bona fide hedge transactions in certain securities. A bona fide hedge...

  1. 17 CFR 240.11a1-3(T) - Bona fide hedge transactions in certain securities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Bona fide hedge transactions in certain securities. 240.11a1-3(T) Section 240.11a1-3(T) Commodity and Securities Exchanges... (rule 11a-1) § 240.11a1-3(T) Bona fide hedge transactions in certain securities. A bona fide hedge...

  2. Centrosome Hypertrophy Induced by p53 Mutations Leads to Tumor Aneuploidy

    DTIC Science & Technology

    1999-06-01

    6). A tripolar mitosis is shown in Figure 6A. Tracings of microtubules, spindle poles, and condensed chromosomes from six non-adjacent serial sections...Centrin Immunofluorescence. A. This section through a symmetrical tripolar mitotic cell shows part of the metaphase plate and portions of the tripolar ...A tripolar metaphase cell immunolabeled with Ki-67 is shown in this tumor section. E. In normal breast epithelium, the centrosomes appear as distinct

  3. Mps1 phosphorylation sites regulate the function of centrin 2 in centriole assembly.

    PubMed

    Yang, Ching-Hui; Kasbek, Christopher; Majumder, Shubhra; Yusof, Adlina Mohd; Fisk, Harold A

    2010-12-01

    The nondegradable Mps1(Δ12/13) protein drives centriole overproduction, suggesting that Mps1 phosphorylates a subset of centrosomal proteins to drive the assembly of new centrioles. Here we identify three Mps1 phosphorylation sites within the centriolar protein Centrin 2 (Cetn2). Although centrioles can be assembled in the absence of Cetn2, centriole assembly is attenuated in the absence of Cetn2. While wild-type Cetn2 can compensate for this attenuation, a nonphosphorylatable version cannot. In addition, overexpressing Cetn2 causes Mps1-dependent centriole overproduction that requires each of the three Mps1 phosphorylation sites within Cetn2 and is greatly exacerbated by mimicking phosphorylation at any of these sites. Wild-type Cetn2 generates excess foci that are competent as mitotic spindle poles in HsSas-6-depleted cells, suggesting that Cetn2 can organize a subset of centriolar proteins independently of cartwheels. However, centriole overproduction caused by a phosphomimetic Cetn2 mutant requires HsSas-6, suggesting that Cetn2 phosphorylation stimulates the canonical centriole assembly pathway. Moreover, in the absence of Cetn2, Mps1(Δ12/13) cannot drive the production of mature centrioles capable of recruiting γ-Tubulin, and a nonphosphorylatable Cetn2 mutant cannot compensate for this defect and exacerbates Cetn2 depletion. Together, our data suggest that Mps1-dependent phosphorylation of Cetn2 stimulates the canonical centriole assembly pathway.

  4. 7 CFR 27.93 - Bona fide spot markets.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Bona fide spot markets. 27.93 Section 27.93... REGULATIONS COTTON CLASSIFICATION UNDER COTTON FUTURES LEGISLATION Regulations Spot Markets § 27.93 Bona fide spot markets. The following markets have been determined, after investigation, and are hereby...

  5. 29 CFR 1606.4 - The bona fide occupational qualification exception.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 4 2011-07-01 2011-07-01 false The bona fide occupational qualification exception. 1606.4 Section 1606.4 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION GUIDELINES ON DISCRIMINATION BECAUSE OF NATIONAL ORIGIN § 1606.4 The bona fide occupational qualification...

  6. 29 CFR 1606.4 - The bona fide occupational qualification exception.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false The bona fide occupational qualification exception. 1606.4 Section 1606.4 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION GUIDELINES ON DISCRIMINATION BECAUSE OF NATIONAL ORIGIN § 1606.4 The bona fide occupational qualification...

  7. 29 CFR 1606.4 - The bona fide occupational qualification exception.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 4 2013-07-01 2013-07-01 false The bona fide occupational qualification exception. 1606.4 Section 1606.4 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION GUIDELINES ON DISCRIMINATION BECAUSE OF NATIONAL ORIGIN § 1606.4 The bona fide occupational qualification...

  8. 29 CFR 1606.4 - The bona fide occupational qualification exception.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 4 2014-07-01 2014-07-01 false The bona fide occupational qualification exception. 1606.4 Section 1606.4 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION GUIDELINES ON DISCRIMINATION BECAUSE OF NATIONAL ORIGIN § 1606.4 The bona fide occupational qualification...

  9. 29 CFR 1606.4 - The bona fide occupational qualification exception.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 4 2012-07-01 2012-07-01 false The bona fide occupational qualification exception. 1606.4 Section 1606.4 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION GUIDELINES ON DISCRIMINATION BECAUSE OF NATIONAL ORIGIN § 1606.4 The bona fide occupational qualification...

  10. 29 CFR 785.19 - Meal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Meal. 785.19 Section 785.19 Labor Regulations Relating to... INTERPRETATION NOT DIRECTLY RELATED TO REGULATIONS HOURS WORKED Application of Principles Rest and Meal Periods § 785.19 Meal. (a) Bona fide meal periods. Bona fide meal periods are not worktime. Bona fide meal...

  11. 29 CFR 785.19 - Meal.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Meal. 785.19 Section 785.19 Labor Regulations Relating to... INTERPRETATION NOT DIRECTLY RELATED TO REGULATIONS HOURS WORKED Application of Principles Rest and Meal Periods § 785.19 Meal. (a) Bona fide meal periods. Bona fide meal periods are not worktime. Bona fide meal...

  12. 17 CFR 1.48 - Requirements for classification of sales or purchases for future delivery as bona fide hedging of...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... classification of sales or purchases for future delivery as bona fide hedging of unsold anticipated production or... for future delivery as bona fide hedging of unsold anticipated production or unfilled anticipated... anticipated production or unfilled anticipated requirements described in these statements shall not be...

  13. 7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

  14. 7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

  15. 7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

  16. 7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

  17. 7 CFR 15a.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Sex as a bona-fide occupational qualification. 15a.61... RECEIVING OR BENEFITTING FROM FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs and Activities Prohibited § 15a.61 Sex as a bona-fide occupational...

  18. 7 CFR 27.96 - Quotations in bona fide spot markets.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Quotations in bona fide spot markets. 27.96 Section 27... Differences § 27.96 Quotations in bona fide spot markets. The price or value and differences between the price... determined by the sale of spot cotton in such spot market. Quotations shall be determined and maintained in...

  19. 17 CFR Appendix B to Part 151 - Examples of Bona Fide Hedging Transactions and Positions

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... fide hedge in a physical-delivery Referenced Contract during the spot month. b. Continuation of Fact... Contract during the spot month. 2. Sovereigns a. Fact Pattern: A Sovereign induces a farmer to sell his...), the risk reducing position will not qualify as a bona fide hedge during the spot month of the physical...

  20. 17 CFR Appendix B to Part 151 - Examples of Bona Fide Hedging Transactions and Positions

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... fide hedge in a physical-delivery Referenced Contract during the spot month. b. Continuation of Fact... Contract during the spot month. 2. Sovereigns a. Fact Pattern: A Sovereign induces a farmer to sell his...), the risk reducing position will not qualify as a bona fide hedge during the spot month of the physical...

  1. 76 FR 73682 - Agency Information Collection Activities; Submission for OMB Review; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ... of a Bona Fide Profit-Sharing Plan or Trust ACTION: Notice. SUMMARY: The Department of Labor (DOL) is..., ``Requirements of a Bona Fide Thrift or Savings Plan and Requirements of a Bona Fide Profit-Sharing Plan or Trust... with the Paperwork Reduction Act (PRA) of 1995 (44 U.S.C. 3501 et seq.). DATES: Submit comments on or...

  2. Binding of calcium and target peptide to calmodulin-like protein CML19, the centrin 2 of Arabidopsis thaliana.

    PubMed

    La Verde, Valentina; Trande, Matteo; D'Onofrio, Mariapina; Dominici, Paola; Astegno, Alessandra

    2018-03-01

    Calmodulin-like protein 19 (CML19) is an Arabidopsis centrin that modulates nucleotide excision repair (NER) by binding to RAD4 protein, the Arabidopsis homolog of human Xeroderma pigmentosum complementation group C protein. Although the necessity of CML19 as a part of the RAD4 plant recognition complex for functional NER is known at a cellular level, little is known at a molecular level. Herein, we used a combination of biophysical and biochemical approaches to investigate the structural and ion and target-peptide binding properties of CML19. We found that CML19 possesses four Ca 2+ -specific binding sites, two of high affinity in the N-terminal domain and two of low affinity in the C-terminal domain. Binding of Ca 2+ to CML19 increases its alpha-helix content, stabilizes the tertiary structure, and triggers a conformational change, resulting in the exposure of a hydrophobic patch instrumental for target protein recognition. Using bioinformatics tools we identified a CML19-binding site at the C-terminus of RAD4, and through in vitro binding experiments we analyzed the interaction between a 17-mer peptide representing this site and CML19. We found that the peptide shows a high affinity for CML19 in the presence of Ca 2+ (stoichiometry 1:1) and the interaction primarily involves the C-terminal half of CML19. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Risk of Sprint Fidelis defibrillator lead failure is highly dependent on age.

    PubMed

    Girerd, Nicolas; Nonin, Emilie; Pinot, Julien; Morel, Elodie; Flys, Carine; Scridon, Alina; Chevalier, Philippe

    2011-01-01

    In 2007, Medtronic Sprint Fidelis defibrillator leads were taken off the market due to a high rate of lead failure. Current data do not allow for risk stratification of patients with regard to lead failure. We sought to determine predictors of Sprint Fidelis lead failure. Between 2004 and 2007, 269 Sprint Fidelis leads were implanted in 258 patients in our centre. Variables associated with lead failure were assessed by the Kaplan-Meier method and a Cox survival model. During a median follow-up of 2.80 years (maximum 5.32), we observed 33 (12.3%) Sprint Fidelis lead failures (5-year survival, 65.6% ± 7.5%). In univariate analysis, age was the only predictor of lead failure (hazard ratio [HR] for 1-year increase 0.97; 95% confidence interval [CI] 0.95-0.99; p=0.009). Patients aged<62.5 years (median) had a significantly increased risk of lead failure compared with patients aged>62.5 years (HR 2.80; CI 1.30-6.02; p=0.009). Survival without Sprint Fidelis lead failure was 55.6% ± 10.4%) in patients aged<62.5 years (24/134 leads) vs 78.6% ± 8.8% in patients aged>62.5 years (9/135 leads). The annual incidence of lead failure in patients aged<62.5 years was 11.6% ± 4.9% during the fourth year after implantation and 22.9% ± 13.2% during the fifth year. Overall, we found a higher rate of Sprint Fidelis lead dysfunction than previously described. Lead failure was much more frequent in younger patients. Our results emphasize the need for close follow-up of younger patients with Sprint Fidelis leads and suggest that, in these patients, the implantation of a new implantable cardioverter defibrillator lead at the time of generator replacement might be reasonable. Copyright © 2011 Elsevier Masson SAS. All rights reserved.

  4. Comparison of Sprint Fidelis and Riata defibrillator lead failure rates.

    PubMed

    Fazal, Iftikhar A; Shepherd, Ewen J; Tynan, Margaret; Plummer, Christopher J; McComb, Janet M

    2013-09-30

    Sprint Fidelis and Riata defibrillator leads are prone to early failure. Few data exist on the comparative failure rates and mortality related to lead failure. The aims of this study were to determine the failure rate of Sprint Fidelis and Riata leads, and to compare failure rates and mortality rates in both groups. Patients implanted with Sprint Fidelis leads and Riata leads at a single centre were identified and in July 2012, records were reviewed to ascertain lead failures, deaths, and relationship to device/lead problems. 113 patients had Sprint Fidelis leads implanted between June 2005 and September 2007; Riata leads were implanted in 106 patients between January 2003 and February 2008. During 53.0 ± 22.3 months of follow-up there were 13 Sprint Fidelis lead failures (11.5%, 2.60% per year) and 25 deaths. Mean time to failure was 45.1 ± 15.5 months. In the Riata lead cohort there were 32 deaths, and 13 lead failures (11.3%, 2.71% per year) over 54.8 ± 26.3 months follow-up with a mean time to failure of 53.5 ± 24.5 months. There were no significant differences in the lead failure-free Kaplan-Meier survival curve (p=0.77), deaths overall (p=0.17), or deaths categorised as sudden/cause unknown (p=0.54). Sprint Fidelis and Riata leads have a significant but comparable failure rate at 2.60% per year and 2.71% per year of follow-up respectively. The number of deaths in both groups is similar and no deaths have been identified as being related to lead failure in either cohort. Copyright © 2012. Published by Elsevier Ireland Ltd.

  5. Gamma-tubulin-containing abnormal centrioles are induced by insufficient Plk4 in human HCT116 colorectal cancer cells.

    PubMed

    Kuriyama, Ryoko; Bettencourt-Dias, Monica; Hoffmann, Ingrid; Arnold, Marc; Sandvig, Lisa

    2009-06-15

    Cancer cells frequently induce aberrant centrosomes, which have been implicated in cancer initiation and progression. Human colorectal cancer cells, HCT116, contain aberrant centrioles composed of disorganized cylindrical microtubules and displaced appendages. These cells also express unique centrosome-related structures associated with a subset of centrosomal components, including gamma-tubulin, centrin and PCM1. During hydroxyurea treatment, these abnormal structures become more abundant and undergo a change in shape from small dots to elongated fibers. Although gamma-tubulin seems to exist as a ring complex, the abnormal structures do not support microtubule nucleation. Several lines of evidence suggest that the fibers correspond to a disorganized form of centriolar microtubules. Plk4, a mammalian homolog of ZYG-1 essential for initiation of centriole biogenesis, is not associated with the gamma-tubulin-specific abnormal centrosomes. The amount of Plk4 at each centrosome was less in cells with abnormal centrosomes than cells without gamma-tubulin-specific abnormal centrosomes. In addition, the formation of abnormal structures was abolished by expression of exogenous Plk4, but not SAS6 and Cep135/Bld10p, which are downstream regulators required for the organization of nine-triplet microtubules. These results suggest that HCT116 cells fail to organize the ninefold symmetry of centrioles due to insufficient Plk4.

  6. 47 CFR 73.1941 - Equal opportunities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Bona fide news interview; (3) Bona fide news documentary (if the appearance of the candidate is... has become a legally qualified candidate for the office in question. (d) Burden of proof. A candidate...

  7. 47 CFR 73.1941 - Equal opportunities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) Bona fide news interview; (3) Bona fide news documentary (if the appearance of the candidate is... has become a legally qualified candidate for the office in question. (d) Burden of proof. A candidate...

  8. Guidance for Avoiding Incomplete Premanufacture Notices or Bona Fides in the New Chemicals Program

    EPA Pesticide Factsheets

    This page contains documents to help you avoid having an incomplete Premanufacture notice or Bona Fide . The documents go over the chemical identity requirements and common errors that result in incompletes.

  9. 7 CFR 905.23 - Selection.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... affiliated with bona fide cooperative marketing organizations. (b) From the nominations made pursuant to... Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... alternates to represent bona fide cooperative marketing organizations which are handlers, and the remaining...

  10. 47 CFR 76.205 - Origination cablecasts by legally qualified candidates for public office; equal opportunities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) Bona fide news interview; (3) Bona fide news documentary (if the appearance of the candidate is... legally qualified candidate for the office in question. (d) Burden of proof. A candidate requesting equal...

  11. 47 CFR 76.205 - Origination cablecasts by legally qualified candidates for public office; equal opportunities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Bona fide news interview; (3) Bona fide news documentary (if the appearance of the candidate is... legally qualified candidate for the office in question. (d) Burden of proof. A candidate requesting equal...

  12. Role of four conserved aspartic acid residues of EF-loops in the metal ion binding and in the self-assembly of ciliate Euplotes octocarinatus centrin.

    PubMed

    Liu, Wen; Duan, Lian; Sun, Tijian; Yang, Binsheng

    2016-12-01

    Ciliate Euplotes octocarinatus centrin (EoCen) is an EF-hand calcium-binding protein closely related to the prototypical calcium sensor protein calmodulin. Four mutants (D37K, D73K, D110K and D146K) were created firstly to elucidate the importance of the first aspartic acid residues (Asp37, Asp73, Asp110 and Asp146) in the beginning of the four EF-loops of EoCen. Aromatic-sensitized Tb 3+ fluorescence indicates that the aspartic acid residues are very important for the metal-binding of EoCen, except for Asp73 (in EF-loop II). Resonance light scattering (RLS) measurements for different metal ions (Ca 2+ and Tb 3+ ) binding proteins suggest that the order of four conserved aspartic acid residues for contributing to the self-assembly of EoCen is Asp37 > Asp146 > Asp110 > Asp73. Cross-linking experiment also exhibits that Asp37 and Asp146 play critical role in the self-assembly of EoCen. Asp37, in site I, which is located in the N-terminal domain, plays the most important role in the metal ion-dependent self-assembly of EoCen, and there is cooperativity between N-terminal and C-terminal domain (especially the site IV). In addition, the dependence of Tb 3+ induced self-assembly of EoCen and the mutants on various factors, including ionic strength and pH, were characterized using RLS. Finally, 2-p-toluidinylnaphthalene-6-sulfonate (TNS) binding, ionic strength and pH control experiments indicate that in the process of EoCen self-assembly, molecular interactions are mediated by both electrostatic and hydrophobic forces, and the hydrophobic interaction has the important status.

  13. Inappropriate implantable cardioverter defibrillator shocks in fractured Sprint Fidelis leads associated with 'appropriate' interrogation.

    PubMed

    Farwell, David; Redpath, Calum; Birnie, David; Gollob, Michael; Lemery, Robert; Posan, Emoke; Green, Martin

    2008-06-01

    We present two patients with fractures within the pace-sense circuit of their Medtronic Sprint Fidelis leads who received inappropriate shocks from their Medtronic defibrillators during device interrogation. This was not simply a coincidence, but due to electromagnetic interference induced within the Sprint Fidelis lead by the device programmer during two-way communication with the defibrillator. Our subsequent investigations have uncovered at least two other similar incidents in Canada. We have also discovered that the Medtronic 'Auto-resume' feature may leave future patients uniquely vulnerable to such inappropriate shocks in the future.

  14. The nucleolar protein SURF-6 is essential for viability in mouse NIH/3T3 cells.

    PubMed

    Polzikov, Mikhail; Magoulas, Charalambos; Zatsepina, Olga

    2007-09-01

    SURF-6 is a bona fide nucleolar protein comprising an evolutionary conserved family that extends from human to yeast. The expression of the mammalian SURF-6 has been recently found to be regulated during the cell cycle. In order to determine the importance of SURF-6 in mammalian cells, we applied the Tet-On system to regulate conditionally, in response to tetracycline, the expression of an antisense RNA (asRNA) that targets Surf-6 mRNA in mouse NIH/3T3 cells. Induced Surf-6 asRNA caused an effective depletion of SURF-6 protein resulted in cell death and in an apparent arrest in the G1 phase of the cell cycle. These results provide for the first time evidence that expression of SURF-6 is essential for mammalian cell viability, and suggest that SURF-6 might participate in the progression of cell cycle.

  15. 29 CFR 1625.6 - Bona fide occupational qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... alternative which would better advance it or equally advance it with less discriminatory impact. (c) Many....6 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.6 Bona fide occupational qualifications. (a) Whether...

  16. 29 CFR 1625.6 - Bona fide occupational qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... alternative which would better advance it or equally advance it with less discriminatory impact. (c) Many....6 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.6 Bona fide occupational qualifications. (a) Whether...

  17. 29 CFR 1625.6 - Bona fide occupational qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... alternative which would better advance it or equally advance it with less discriminatory impact. (c) Many....6 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.6 Bona fide occupational qualifications. (a) Whether...

  18. 29 CFR 1625.6 - Bona fide occupational qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... alternative which would better advance it or equally advance it with less discriminatory impact. (c) Many....6 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.6 Bona fide occupational qualifications. (a) Whether...

  19. 29 CFR 1625.6 - Bona fide occupational qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... alternative which would better advance it or equally advance it with less discriminatory impact. (c) Many....6 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.6 Bona fide occupational qualifications. (a) Whether...

  20. Age Discrimination by Local Governmental Entities--The Defense of a Bona Fide Occupational Qualification.

    ERIC Educational Resources Information Center

    Hujar, Frances B.

    1979-01-01

    The bona fide occupational qualification defense is interpreted narrowly and is most likely to be applied in job situations that involve the safety of others. Available from Baylor Law Review, B.U. Station, Box 6262, Waco, TX 76706. (Author/IRT)

  1. The plant i-AAA protease controls the turnover of an essential mitochondrial protein import component.

    PubMed

    Opalińska, Magdalena; Parys, Katarzyna; Murcha, Monika W; Jańska, Hanna

    2018-01-29

    Mitochondria are multifunctional organelles that play a central role in energy metabolism. Owing to the life-essential functions of these organelles, mitochondrial content, quality and dynamics are tightly controlled. Across the species, highly conserved ATP-dependent proteases prevent malfunction of mitochondria through versatile activities. This study focuses on a molecular function of the plant mitochondrial inner membrane-embedded AAA protease (denoted i -AAA) FTSH4, providing its first bona fide substrate. Here, we report that the abundance of the Tim17-2 protein, an essential component of the TIM17:23 translocase (Tim17-2 together with Tim50 and Tim23), is directly controlled by the proteolytic activity of FTSH4. Plants that are lacking functional FTSH4 protease are characterized by significantly enhanced capacity of preprotein import through the TIM17:23-dependent pathway. Taken together, with the observation that FTSH4 prevents accumulation of Tim17-2, our data point towards the role of this i -AAA protease in the regulation of mitochondrial biogenesis in plants. © 2018. Published by The Company of Biologists Ltd.

  2. 24 CFR 3.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Sex as a bona fide occupational... and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  3. 32 CFR 196.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  4. 14 CFR § 1253.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Sex as a bona fide occupational... ADMINISTRATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

  5. 45 CFR 2555.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 4 2014-10-01 2014-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  6. 32 CFR 196.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 2 2014-07-01 2014-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  7. 45 CFR 2555.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 4 2013-10-01 2013-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  8. 24 CFR 3.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Sex as a bona fide occupational... and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  9. 36 CFR 1211.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Sex as a bona fide... RECORDS ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  10. 45 CFR 618.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

  11. 38 CFR 23.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Sex as a bona fide... VETERANS AFFAIRS (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  12. 45 CFR 2555.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  13. 24 CFR 3.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Sex as a bona fide occupational... and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  14. 13 CFR 113.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Sex as a bona fide occupational... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Employment in Education Programs Or Activities...

  15. 38 CFR 23.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Sex as a bona fide... VETERANS AFFAIRS (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  16. 32 CFR 196.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  17. 24 CFR 3.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Sex as a bona fide occupational... and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  18. 44 CFR 19.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Sex as a bona fide... MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  19. 44 CFR 19.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 44 Emergency Management and Assistance 1 2014-10-01 2014-10-01 false Sex as a bona fide... MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  20. 24 CFR 3.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Sex as a bona fide occupational... and Urban Development NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  1. 10 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  2. 45 CFR 2555.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 4 2012-10-01 2012-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  3. 45 CFR 2555.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Sex as a bona fide occupational qualification... FOR NATIONAL AND COMMUNITY SERVICE NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  4. 44 CFR 19.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 44 Emergency Management and Assistance 1 2011-10-01 2011-10-01 false Sex as a bona fide... MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  5. 32 CFR 196.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  6. 45 CFR 618.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

  7. 32 CFR 196.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 2 2012-07-01 2012-07-01 false Sex as a bona fide occupational qualification... DEFENSE (CONTINUED) MISCELLANEOUS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  8. 10 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  9. 36 CFR 1211.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Sex as a bona fide... RECORDS ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  10. 44 CFR 19.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 44 Emergency Management and Assistance 1 2013-10-01 2013-10-01 false Sex as a bona fide... MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  11. 13 CFR 113.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Sex as a bona fide occupational... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Employment in Education Programs Or Activities...

  12. 45 CFR 618.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

  13. 13 CFR 113.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Sex as a bona fide occupational... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Employment in Education Programs Or Activities...

  14. 45 CFR 618.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

  15. 44 CFR 19.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 44 Emergency Management and Assistance 1 2012-10-01 2011-10-01 true Sex as a bona fide... MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  16. 10 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  17. 36 CFR 1211.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Sex as a bona fide... RECORDS ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  18. 36 CFR § 1211.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Sex as a bona fide... ARCHIVES AND RECORDS ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  19. 13 CFR 113.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Sex as a bona fide occupational... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Employment in Education Programs Or Activities...

  20. 10 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  1. 38 CFR 23.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Sex as a bona fide... VETERANS AFFAIRS (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  2. 45 CFR 618.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Sex as a bona fide occupational qualification. 618... FOUNDATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

  3. 13 CFR 113.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Sex as a bona fide occupational... ADMINISTRATOR Nondiscrimination on the Basis of Sex in Education Programs or Activities Receiving Federal Financial Assistance Discrimination on the Basis of Sex in Employment in Education Programs Or Activities...

  4. 36 CFR 1211.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Sex as a bona fide... RECORDS ADMINISTRATION GENERAL RULES NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in...

  5. 38 CFR 23.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Sex as a bona fide... VETERANS AFFAIRS (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  6. 10 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Sex as a bona fide occupational qualification. 5.550 Section 5.550 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  7. 38 CFR 23.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Sex as a bona fide... VETERANS AFFAIRS (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education...

  8. 29 CFR 1625.12 - Exemption for bona fide executive or high policymaking employees.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... corporations [or other business organizations] are usually located at corporate or regional headquarters. With respect to employees whose duties are associated with corporate headquarters operations, such as finance..., would be covered by the term “bona fide executive.” Individuals at higher levels in the corporate...

  9. 78 FR 9330 - Revision of Regulations Defining Bona Fide Cotton Spot Markets

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-08

    ... Cotton Spot Markets AGENCY: Agricultural Marketing Service, USDA. ACTION: Proposed rule. SUMMARY: The... bona fide cotton spot markets in order to assure consistency with the revised Cotton Research and... spot prices of cotton marketed in Kansas and Virginia. AMS is also proposing to amend references to the...

  10. 26 CFR 1.409A-3 - Permissible payments.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... schedule of periodic payments on a dollar-for-dollar basis by the amount of bona fide disability pay..., objective formula limitation, if the disability payments are made pursuant to a plan sponsored by the... other change in the benefit payable under, such bona fide disability plan results in an acceleration of...

  11. The Tregs' world according to GARP.

    PubMed

    Battaglia, Manuela; Roncarolo, Maria Grazia

    2009-12-01

    Naturally occurring CD4+CD25(high) regulatory T cells (nTreg) are essential for maintaining tolerance. FOXP3 has been established as a molecular marker of nTreg; however, FOXP3 cannot be used as a reliable marker for bona fide human nTreg since effector T cells also up-regulate FOXP3 expression upon activation. Despite the important function of nTreg, the underlying molecular mechanisms of nTreg-mediated suppression are far from defined. Previous studies have demonstrated that the TGF-beta latency-associated peptide (LAP) is expressed on the surface of nTreg, and that immunosuppression can be mediated by membrane TGF-beta; however, it remains unknown how LAP is bound to nTreg and what is the functional significance of its selective expression on activated nTreg. The nTreg's world may now change according to GARP, an orphan toll-like receptor composed of leucine-rich repeats. In this issue of the European Journal of Immunology, a study provides further demonstration that GARP is selectively expressed only in activated human nTreg and nTreg cell clones but not in activated effector T cells, confirming GARP as a bona fide nTreg marker. In addition, GARP binds directly to LAP; yet, GARP over-expression is insufficient to induce modification of latent TGF-beta into active TGF-beta further clarifying its role in nTreg-mediated suppression.

  12. 29 CFR 1625.12 - Exemption for bona fide executive or high policymaking employees.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... employees. 1625.12 Section 1625.12 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.12 Exemption for bona fide... which equals, in the aggregate, at least $44,000. (b) Since this provision is an exemption from the non...

  13. 29 CFR 1625.12 - Exemption for bona fide executive or high policymaking employees.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... employees. 1625.12 Section 1625.12 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.12 Exemption for bona fide... which equals, in the aggregate, at least $44,000. (b) Since this provision is an exemption from the non...

  14. 29 CFR 1625.12 - Exemption for bona fide executive or high policymaking employees.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... employees. 1625.12 Section 1625.12 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.12 Exemption for bona fide... which equals, in the aggregate, at least $44,000. (b) Since this provision is an exemption from the non...

  15. 29 CFR 1625.12 - Exemption for bona fide executive or high policymaking employees.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... employees. 1625.12 Section 1625.12 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN EMPLOYMENT ACT Interpretations § 1625.12 Exemption for bona fide... which equals, in the aggregate, at least $44,000. (b) Since this provision is an exemption from the non...

  16. 17 CFR 240.16b-5 - Bona fide gifts and inheritance.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Bona fide gifts and inheritance. 240.16b-5 Section 240.16b-5 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION... gifts and inheritance. Both the acquisition and the disposition of equity securities shall be exempt...

  17. 39 CFR 320.4 - Suspension for certain letters of college and university organizations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... universities to carry in their internal mail systems the letters of their bona fide student or faculty... university. Colleges and universities choosing to provide their student or faculty organizations access to their internal mail systems are responsible for assuring that only letters of bona fide student or...

  18. 29 CFR 36.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 1 2012-07-01 2012-07-01 false Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  19. 49 CFR 25.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

  20. 14 CFR 1253.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 1253.550 Sex as...

  1. 14 CFR 1253.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 1253.550 Sex as...

  2. 18 CFR 1317.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Sex as a bona fide... VALLEY AUTHORITY NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  3. 28 CFR 54.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Sex as a bona fide occupational... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 54.550 Sex as a...

  4. 22 CFR 229.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

  5. 49 CFR 25.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

  6. 22 CFR 229.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

  7. 18 CFR 1317.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Sex as a bona fide... VALLEY AUTHORITY NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  8. 22 CFR 146.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

  9. 28 CFR 54.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Sex as a bona fide occupational... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 54.550 Sex as a...

  10. 22 CFR 146.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

  11. 10 CFR 1042.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

  12. 22 CFR 146.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

  13. 34 CFR 106.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  14. 49 CFR 25.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

  15. 34 CFR 106.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 1 2013-07-01 2013-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  16. 34 CFR 106.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 1 2012-07-01 2012-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  17. 18 CFR 1317.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Sex as a bona fide... VALLEY AUTHORITY NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  18. 14 CFR 1253.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 1253.550 Sex as...

  19. 29 CFR 36.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 1 2011-07-01 2011-07-01 false Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  20. 28 CFR 54.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Sex as a bona fide occupational... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 54.550 Sex as a...

  1. 34 CFR 106.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  2. 49 CFR 25.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

  3. 29 CFR 36.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 1 2013-07-01 2013-07-01 false Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  4. 22 CFR 146.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

  5. 22 CFR 229.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

  6. 34 CFR 106.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 1 2014-07-01 2014-07-01 false Sex as a bona-fide occupational qualification. 106.61..., DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  7. 29 CFR 36.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 1 2014-07-01 2013-07-01 true Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  8. 18 CFR 1317.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Sex as a bona fide... VALLEY AUTHORITY NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  9. 28 CFR 54.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Sex as a bona fide occupational... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 54.550 Sex as a...

  10. 14 CFR 1253.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 1253.550 Sex as...

  11. 22 CFR 146.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Sex as a bona fide occupational qualification... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.550 Sex as a...

  12. 10 CFR 1042.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

  13. 49 CFR 25.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 1 2014-10-01 2014-10-01 false Sex as a bona fide occupational qualification. 25... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 25.550 Sex as a...

  14. 29 CFR 36.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Sex as a bona fide occupational qualification. 36.550 Section 36.550 Labor Office of the Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in...

  15. 10 CFR 1042.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

  16. 22 CFR 229.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

  17. 28 CFR 54.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Sex as a bona fide occupational... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 54.550 Sex as a...

  18. 18 CFR 1317.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false Sex as a bona fide... VALLEY AUTHORITY NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or...

  19. 10 CFR 1042.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

  20. 10 CFR 1042.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Sex as a bona fide occupational qualification. 1042.550 Section 1042.550 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex...

  1. 22 CFR 229.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Sex as a bona fide occupational qualification... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.550 Sex as...

  2. 42 CFR 433.67 - Limitations on level of FFP for permissible provider-related donations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... provider-related donations. 433.67 Section 433.67 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES... permissible provider-related donations. (a)(1) Limitations on bona fide donations. There are no limitations on the amount of bona fide provider-related donations that a State may receive without a reduction in FFP...

  3. 42 CFR 433.67 - Limitations on level of FFP for permissible provider-related donations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... provider-related donations. 433.67 Section 433.67 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES... permissible provider-related donations. (a)(1) Limitations on bona fide donations. There are no limitations on the amount of bona fide provider-related donations that a State may receive without a reduction in FFP...

  4. 29 CFR 452.80 - Bona fide candidates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... nominated in order to be a bona fide candidate entitled to exercise the rights mentioned in §§ 452.67 and... able to take advantage of the distribution rights even before the convention meets and thus attempt to.... United Mine Workers, 71 LRRM 2606, 60 L.C. 10,204 (D.D.C. 1969). ...

  5. 20 CFR 617.44 - Findings required.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... individual began and completed the relocation within the limitations specified in § 617.42(a)(7) and § 617.43... employment of long-term duration, or a bona fide offer of such suitable employment, in the area of intended... employment affording a reasonable expectation of employment of long-term duration, or a bona fide offer of...

  6. 5 CFR 550.112 - Computation of overtime work.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... meal time. (1) Bona fide sleep and meal periods may not be considered hours of work, except as provided... a call to duty, the time spent on duty is hours of work. (2) Sleep and meal periods during regularly....C. 5545(c)(1), the amount of bona fide sleep and meal time excluded from hours of work may not...

  7. 5 CFR 550.112 - Computation of overtime work.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... meal time. (1) Bona fide sleep and meal periods may not be considered hours of work, except as provided... a call to duty, the time spent on duty is hours of work. (2) Sleep and meal periods during regularly....C. 5545(c)(1), the amount of bona fide sleep and meal time excluded from hours of work may not...

  8. 6 CFR 17.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

  9. 31 CFR 28.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance: Treasury 1 2013-07-01 2013-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 28.550 Sex as a...

  10. 31 CFR 28.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance: Treasury 1 2014-07-01 2014-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 28.550 Sex as a...

  11. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as a...

  12. 43 CFR 41.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 41.550 Sex as a...

  13. 31 CFR 28.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance: Treasury 1 2012-07-01 2012-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 28.550 Sex as a...

  14. 41 CFR 101-4.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 2 2012-07-01 2012-07-01 false Sex as a bona fide... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 101-4.550 Sex...

  15. 43 CFR 41.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 41.550 Sex as a...

  16. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as a...

  17. 41 CFR 101-4.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 2 2013-07-01 2012-07-01 true Sex as a bona fide... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 101-4.550 Sex...

  18. 41 CFR 101-4.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 41 Public Contracts and Property Management 2 2011-07-01 2007-07-01 true Sex as a bona fide... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 101-4.550 Sex...

  19. 41 CFR 101-4.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 41 Public Contracts and Property Management 2 2014-07-01 2012-07-01 true Sex as a bona fide... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 101-4.550 Sex...

  20. 41 CFR 101-4.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Sex as a bona fide... ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 101-4.550 Sex...

  1. 6 CFR 17.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 6 Domestic Security 1 2013-01-01 2013-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

  2. 45 CFR 86.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 1 2013-10-01 2013-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

  3. 31 CFR 28.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance: Treasury 1 2011-07-01 2011-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 28.550 Sex as a...

  4. 40 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 5.550 Sex as a...

  5. 40 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 5.550 Sex as a...

  6. 45 CFR 86.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

  7. 43 CFR 41.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 41.550 Sex as a...

  8. 43 CFR 41.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 41.550 Sex as a...

  9. 45 CFR 86.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 1 2011-10-01 2011-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

  10. 40 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 5.550 Sex as a...

  11. 6 CFR 17.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 6 Domestic Security 1 2014-01-01 2014-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

  12. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as a...

  13. 40 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 5.550 Sex as a...

  14. 6 CFR 17.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 6 Domestic Security 1 2011-01-01 2011-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

  15. 45 CFR 86.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 1 2014-10-01 2014-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

  16. 40 CFR 5.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 5.550 Sex as a...

  17. 45 CFR 86.61 - Sex as a bona-fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 1 2012-10-01 2012-10-01 false Sex as a bona-fide occupational qualification. 86... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 86.61 Sex as a...

  18. 6 CFR 17.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 6 Domestic Security 1 2012-01-01 2012-01-01 false Sex as a bona fide occupational qualification... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 17.550 Sex as a...

  19. 31 CFR 28.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 28.550 Sex as a...

  20. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as a...

  1. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as a...

  2. 43 CFR 41.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 41.550 Sex as a...

  3. 76 FR 78062 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-15

    ... problem and make other technical changes. The proposed change will be operative on December 1, 2011. The... experiences a bona fide systems problem and make other technical changes. The Exchange presently has three... Exchange experienced a bona fide systems problem. The Exchange proposes to amend the Fee Schedule to extend...

  4. 77 FR 54630 - Self-Regulatory Organizations; BATS-Y Exchange, Inc.; Order Approving Proposed Rule Change, as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ... maker quoter functionality optionally creates a quotation for display to comply with market making...-designated offset may be able to qualify as bona-fide market making for purposes of Regulation SHO, depending... of Rule 203 for bona-fide market making is appropriate with respect to the particular Market Maker...

  5. 19 CFR 10.153 - Conditions for exemption.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... over $200 and Bona Fide Gifts § 10.153 Conditions for exemption. Customs officers shall be further... § 10.151 or § 10.152: (a) A “bona fide gift” for purposes of § 10.152 is an article formerly owned by a... promise of compensation. It does not include articles acquired by purchase, barter, promissory exchange...

  6. Regulation of DUOX by the Galphaq-phospholipase Cbeta-Ca2+ pathway in Drosophila gut immunity.

    PubMed

    Ha, Eun-Mi; Lee, Kyung-Ah; Park, Seon Hwa; Kim, Sung-Hee; Nam, Hyuck-Jin; Lee, Hyo-Young; Kang, Dongmin; Lee, Won-Jae

    2009-03-01

    All metazoan guts are in constant contact with diverse food-borne microorganisms. The signaling mechanisms by which the host regulates gut-microbe interactions, however, are not yet clear. Here, we show that phospholipase C-beta (PLCbeta) signaling modulates dual oxidase (DUOX) activity to produce microbicidal reactive oxygen species (ROS) essential for normal host survival. Gut-microbe contact rapidly activates PLCbeta through Galphaq, which in turn mobilizes intracellular Ca(2+) through inositol 1,4,5-trisphosphate generation for DUOX-dependent ROS production. PLCbeta mutant flies had a short life span due to the uncontrolled propagation of an essential nutritional microbe, Saccharomyces cerevisiae, in the gut. Gut-specific reintroduction of the PLCbeta restored efficient DUOX-dependent microbe-eliminating capacity and normal host survival. These results demonstrate that the Galphaq-PLCbeta-Ca(2+)-DUOX-ROS signaling pathway acts as a bona fide first line of defense that enables gut epithelia to dynamically control yeast during the Drosophila life cycle.

  7. 29 CFR 4.156 - Employees in bona fide executive, administrative, or professional capacity.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... SERVICE CONTRACTS Application of the McNamara-O'Hara Service Contract Act Employees Covered by the Act § 4... employee as defined in section 8(b) of the Act does not include persons employed in a bona fide executive... requirements of the regulations in part 541 of this title, are ordinarily covered by the Act's provisions...

  8. 76 FR 80337 - Uncovered Innerspring Units From the People's Republic of China: Rescission of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-23

    ... preliminarily found that Foshan Nanhai Jiujiang Quan Li Spring Hardware Factory's (``Quan Li'') sale was non-bona fide, and announced our preliminary intent to rescind Quan Li's NSR. For the final results of this review, we continue to find Quan Li's sale to be non-bona fide. Therefore, because there were no other...

  9. 24 CFR 100.60 - Unlawful refusal to sell or rent or to negotiate for the sale or rental.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... bona fide offer, because of race, color, religion, sex, familial status, or national origin or to refuse to negotiate with a person for the sale or rental of a dwelling because of race, color, religion... limited to: (1) Failing to accept or consider a bona fide offer because of race, color, religion, sex...

  10. 17 CFR 270.10e-1 - Death, disqualification, or bona fide resignation of directors.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... company, by reason of the death, disqualification, or bona fide resignation of any director, does not meet... company's board of directors, the operation of the relevant subsection of the Act, rule, or regulation will be suspended as to the company: (a) For 90 days if the vacancy may be filled by action of the...

  11. 24 CFR 100.60 - Unlawful refusal to sell or rent or to negotiate for the sale or rental.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... bona fide offer, because of race, color, religion, sex, familial status, or national origin or to refuse to negotiate with a person for the sale or rental of a dwelling because of race, color, religion... limited to: (1) Failing to accept or consider a bona fide offer because of race, color, religion, sex...

  12. 24 CFR 100.60 - Unlawful refusal to sell or rent or to negotiate for the sale or rental.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... bona fide offer, because of race, color, religion, sex, familial status, or national origin or to refuse to negotiate with a person for the sale or rental of a dwelling because of race, color, religion... limited to: (1) Failing to accept or consider a bona fide offer because of race, color, religion, sex...

  13. 24 CFR 100.60 - Unlawful refusal to sell or rent or to negotiate for the sale or rental.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... bona fide offer, because of race, color, religion, sex, familial status, or national origin or to refuse to negotiate with a person for the sale or rental of a dwelling because of race, color, religion... limited to: (1) Failing to accept or consider a bona fide offer because of race, color, religion, sex...

  14. 24 CFR 100.60 - Unlawful refusal to sell or rent or to negotiate for the sale or rental.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... bona fide offer, because of race, color, religion, sex, familial status, or national origin or to refuse to negotiate with a person for the sale or rental of a dwelling because of race, color, religion... limited to: (1) Failing to accept or consider a bona fide offer because of race, color, religion, sex...

  15. 76 FR 4289 - Honey From the People's Republic of China: Final Results and Rescission of Antidumping Duty New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ... Fenglian Co., Ltd. (``Fenglian''), the Department finds that that sales under review are not bona fide... Shipper Reviews, 75 FR 55307 (September 10, 2010). Because the sales under review were made during the POR... Republic of China: Bona Fide Analysis of the Sale Under Review for Suzhou Shanding Honey Product Co., Ltd...

  16. 29 CFR 549.2 - Disqualifying provisions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OF A âBONA FIDE PROFIT-SHARING PLAN OR TRUSTâ § 549.2 Disqualifying provisions. No plan or trust... fide profit-sharing plan or trust under section 7(e)(3)(b) of the Act: (a) If the share of any... terms of the plan or trust, is set at a predetermined fixed sum or is so limited as to provide in effect...

  17. Generating Bona Fide Mammalian Prions with Internal Deletions.

    PubMed

    Munoz-Montesino, Carola; Sizun, Christina; Moudjou, Mohammed; Herzog, Laetitia; Reine, Fabienne; Chapuis, Jérôme; Ciric, Danica; Igel-Egalon, Angelique; Laude, Hubert; Béringue, Vincent; Rezaei, Human; Dron, Michel

    2016-08-01

    Mammalian prions are PrP proteins with altered structures causing transmissible fatal neurodegenerative diseases. They are self-perpetuating through formation of beta-sheet-rich assemblies that seed conformational change of cellular PrP. Pathological PrP usually forms an insoluble protease-resistant core exhibiting beta-sheet structures but no more alpha-helical content, loosing the three alpha-helices contained in the correctly folded PrP. The lack of a high-resolution prion structure makes it difficult to understand the dynamics of conversion and to identify elements of the protein involved in this process. To determine whether completeness of residues within the protease-resistant domain is required for prions, we performed serial deletions in the helix H2 C terminus of ovine PrP, since this region has previously shown some tolerance to sequence changes without preventing prion replication. Deletions of either four or five residues essentially preserved the overall PrP structure and mutant PrP expressed in RK13 cells were efficiently converted into bona fide prions upon challenge by three different prion strains. Remarkably, deletions in PrP facilitated the replication of two strains that otherwise do not replicate in this cellular context. Prions with internal deletion were self-propagating and de novo infectious for naive homologous and wild-type PrP-expressing cells. Moreover, they caused transmissible spongiform encephalopathies in mice, with similar biochemical signatures and neuropathologies other than the original strains. Prion convertibility and transfer of strain-specific information are thus preserved despite shortening of an alpha-helix in PrP and removal of residues within prions. These findings provide new insights into sequence/structure/infectivity relationship for prions. Prions are misfolded PrP proteins that convert the normal protein into a replicate of their own abnormal form. They are responsible for invariably fatal neurodegenerative disorders. Other aggregation-prone proteins appear to have a prion-like mode of expansion in brains, such as in Alzheimer's or Parkinson's diseases. To date, the resolution of prion structure remains elusive. Thus, to genetically define the landscape of regions critical for prion conversion, we tested the effect of short deletions. We found that, surprisingly, removal of a portion of PrP, the C terminus of alpha-helix H2, did not hamper prion formation but generated infectious agents with an internal deletion that showed characteristics essentially similar to those of original infecting strains. Thus, we demonstrate that completeness of the residues inside prions is not necessary for maintaining infectivity and the main strain-specific information, while reporting one of the few if not the only bona fide prions with an internal deletion. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  18. Generating Bona Fide Mammalian Prions with Internal Deletions

    PubMed Central

    Munoz-Montesino, Carola; Sizun, Christina; Moudjou, Mohammed; Herzog, Laetitia; Reine, Fabienne; Chapuis, Jérôme; Ciric, Danica; Igel-Egalon, Angelique; Laude, Hubert; Béringue, Vincent; Rezaei, Human

    2016-01-01

    ABSTRACT Mammalian prions are PrP proteins with altered structures causing transmissible fatal neurodegenerative diseases. They are self-perpetuating through formation of beta-sheet-rich assemblies that seed conformational change of cellular PrP. Pathological PrP usually forms an insoluble protease-resistant core exhibiting beta-sheet structures but no more alpha-helical content, loosing the three alpha-helices contained in the correctly folded PrP. The lack of a high-resolution prion structure makes it difficult to understand the dynamics of conversion and to identify elements of the protein involved in this process. To determine whether completeness of residues within the protease-resistant domain is required for prions, we performed serial deletions in the helix H2 C terminus of ovine PrP, since this region has previously shown some tolerance to sequence changes without preventing prion replication. Deletions of either four or five residues essentially preserved the overall PrP structure and mutant PrP expressed in RK13 cells were efficiently converted into bona fide prions upon challenge by three different prion strains. Remarkably, deletions in PrP facilitated the replication of two strains that otherwise do not replicate in this cellular context. Prions with internal deletion were self-propagating and de novo infectious for naive homologous and wild-type PrP-expressing cells. Moreover, they caused transmissible spongiform encephalopathies in mice, with similar biochemical signatures and neuropathologies other than the original strains. Prion convertibility and transfer of strain-specific information are thus preserved despite shortening of an alpha-helix in PrP and removal of residues within prions. These findings provide new insights into sequence/structure/infectivity relationship for prions. IMPORTANCE Prions are misfolded PrP proteins that convert the normal protein into a replicate of their own abnormal form. They are responsible for invariably fatal neurodegenerative disorders. Other aggregation-prone proteins appear to have a prion-like mode of expansion in brains, such as in Alzheimer's or Parkinson's diseases. To date, the resolution of prion structure remains elusive. Thus, to genetically define the landscape of regions critical for prion conversion, we tested the effect of short deletions. We found that, surprisingly, removal of a portion of PrP, the C terminus of alpha-helix H2, did not hamper prion formation but generated infectious agents with an internal deletion that showed characteristics essentially similar to those of original infecting strains. Thus, we demonstrate that completeness of the residues inside prions is not necessary for maintaining infectivity and the main strain-specific information, while reporting one of the few if not the only bona fide prions with an internal deletion. PMID:27226369

  19. Generation of growth arrested Leishmania amastigotes: a tool to develop live attenuated vaccine candidates against visceral leishmaniasis.

    PubMed

    Selvapandiyan, Angamuthu; Dey, Ranadhir; Gannavaram, Sreenivas; Solanki, Sumit; Salotra, Poonam; Nakhasi, Hira L

    2014-06-30

    Visceral leishmaniasis (VL) is fatal if not treated and is prevalent widely in the tropical and sub-tropical regions of world. VL is caused by the protozoan parasite Leishmania donovani or Leishmania infantum. Although several second generation vaccines have been licensed to protect dogs against VL, there are no effective vaccines against human VL [1]. Since people cured of leishmaniasis develop lifelong protection, development of live attenuated Leishmania parasites as vaccines, which can have controlled infection, may be a close surrogate to leishmanization. This can be achieved by deletion of genes involved in the regulation of growth and/or virulence of the parasite. Such mutant parasites generally do not revert to virulence in animal models even under conditions of induced immune suppression due to complete deletion of the essential gene(s). In the Leishmania life cycle, the intracellular amastigote form is the virulent form and causes disease in the mammalian hosts. We developed centrin gene deleted L. donovani parasites that displayed attenuated growth only in the amastigote stage and were found safe and efficacious against virulent challenge in the experimental animal models. Thus, targeting genes differentially expressed in the amastigote stage would potentially attenuate only the amastigote stage and hence controlled infectivity may be effective in developing immunity. This review lays out the strategies for attenuation of the growth of the amastigote form of Leishmania for use as live vaccine against leishmaniasis, with a focus on visceral leishmaniasis. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Metabolic engineering with plants for a sustainable biobased economy.

    PubMed

    Yoon, Jong Moon; Zhao, Le; Shanks, Jacqueline V

    2013-01-01

    Plants are bona fide sustainable organisms because they accumulate carbon and synthesize beneficial metabolites from photosynthesis. To meet the challenges to food security and health threatened by increasing population growth and depletion of nonrenewable natural resources, recent metabolic engineering efforts have shifted from single pathways to holistic approaches with multiple genes owing to integration of omics technologies. Successful engineering of plants results in the high yield of biomass components for primary food sources and biofuel feedstocks, pharmaceuticals, and platform chemicals through synthetic biology and systems biology strategies. Further discovery of undefined biosynthesis pathways in plants, integrative analysis of discrete omics data, and diversified process developments for production of platform chemicals are essential to overcome the hurdles for sustainable production of value-added biomolecules from plants.

  1. 75 FR 55307 - Honey From the People's Republic of China: Preliminary Intent To Rescind New Shipper Reviews

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-10

    ... Shipper Review, 75 FR 38980 (July 7, 2010). Expansion of the POR When the sale of the subject merchandise... review is based on a single sale, exclusion of that sale as non-bona fide necessarily must end the review... Shipper Review of Honey from the People's Republic of China: Bona Fide Analysis of the Sale Under Review...

  2. From Mosques to Classrooms: Mobilizing the Community to Enhance Case Detection of Tuberculosis

    PubMed Central

    Rifat, Mahfuza; Rusen, I. D.; Mahmud, Mohammad Hasan; Nayer, Israt; Islam, Akramul; Ahmed, Faruque

    2008-01-01

    In response to the global challenge of inadequate case detection of tuberculosis (TB), the Fund for Innovative DOTS Expansion through Local Initiatives to Stop Tuberculosis (FIDELIS) was developed in 2003 to rapidly assess and implement innovative approaches to increase the detection of new smear-positive TB cases. As previously reported, a wide range of target populations and interventions has been incorporated into successful FIDELIS projects. PMID:18633095

  3. Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Cheng, Ai-Xia; Zhang, Xuebin; Han, Xiao-Juan

    Flavonoids ubiquitously distribute to the terrestrial plants and chalcone isomerase (CHI)- catalyzed intramolecular and stereospecific cyclization of chalcones is a committed step in the production of flavonoids. However, so far the bona fide CHIs are found only in vascular plants, and their origin and evolution remains elusive. We conducted transcriptomic and/or genomic sequence search, subsequent phylogenetic analysis, and detailed biochemical and genetic characterization to explore the potential existence of CHI proteins in the basal bryophyte liverwort species and the lycophyte Selaginella moellendorffii. We found that both liverwort and Selaginella species possess canonical CHI-fold proteins that cluster with their corresponding highermore » plant counterparts. Among them, some members exhibited bona fide CHI activity, which catalyze stereospecific cyclization of both 60- hydroxychalcone and 60-deoxychalcone, yielding corresponding 5-hydroxy and 5- deoxyflavanones, resembling the typical type II CHIs currently known to be ‘specific’ for legume plants. Expressing those primitive bona fide CHIs in the Arabidopsis chi mutant restores the seed coat transparent testa phenotype and the accumulation of flavonoids. These findings, in contrast to our current understanding of the evolution of enzymatic CHIs, suggest that emergence of the bona fide type II CHIs is an ancient evolution event that occurred before the divergence of liverwort lineages.« less

  4. Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids

    DOE PAGES

    Cheng, Ai-Xia; Zhang, Xuebin; Han, Xiao-Juan; ...

    2017-10-30

    Flavonoids ubiquitously distribute to the terrestrial plants and chalcone isomerase (CHI)- catalyzed intramolecular and stereospecific cyclization of chalcones is a committed step in the production of flavonoids. However, so far the bona fide CHIs are found only in vascular plants, and their origin and evolution remains elusive. We conducted transcriptomic and/or genomic sequence search, subsequent phylogenetic analysis, and detailed biochemical and genetic characterization to explore the potential existence of CHI proteins in the basal bryophyte liverwort species and the lycophyte Selaginella moellendorffii. We found that both liverwort and Selaginella species possess canonical CHI-fold proteins that cluster with their corresponding highermore » plant counterparts. Among them, some members exhibited bona fide CHI activity, which catalyze stereospecific cyclization of both 60- hydroxychalcone and 60-deoxychalcone, yielding corresponding 5-hydroxy and 5- deoxyflavanones, resembling the typical type II CHIs currently known to be ‘specific’ for legume plants. Expressing those primitive bona fide CHIs in the Arabidopsis chi mutant restores the seed coat transparent testa phenotype and the accumulation of flavonoids. These findings, in contrast to our current understanding of the evolution of enzymatic CHIs, suggest that emergence of the bona fide type II CHIs is an ancient evolution event that occurred before the divergence of liverwort lineages.« less

  5. Birth Attendants and Midwifery Practice in Early Twentieth-century Derbyshire

    PubMed Central

    Reid, Alice

    2012-01-01

    Summary The 1902 Midwives Act introduced training and supervision for midwives in England and Wales, outlawing uncertified-and-untrained midwives (handywomen) and phasing out certified-but-untrained (bona fide) midwives. This paper compares the numbers and practices of these two different types of birth attendant with each other, with qualified and certified midwives and with doctors in early twentieth-century Derbyshire during this period of change, and examines the spatial and social factors influencing women's choice of birth attendant. It finds that the new legislation did not entirely eliminate continuity in traditional practices and allegiance, and that both social and spatial factors governed the choice of delivery attendant, with fewer midwives available in rural areas and a surviving network of untrained bona fide midwives in mining communities. Within this spatial pattern, however, although wealthier women were more likely to have chosen a doctor or a qualified midwife, familiarity and loyalty allowed bona fide midwives to maintain their case loads.

  6. Replication Stress: A Lifetime of Epigenetic Change

    PubMed Central

    Khurana, Simran; Oberdoerffer, Philipp

    2015-01-01

    DNA replication is essential for cell division. Challenges to the progression of DNA polymerase can result in replication stress, promoting the stalling and ultimately collapse of replication forks. The latter involves the formation of DNA double-strand breaks (DSBs) and has been linked to both genome instability and irreversible cell cycle arrest (senescence). Recent technological advances have elucidated many of the factors that contribute to the sensing and repair of stalled or broken replication forks. In addition to bona fide repair factors, these efforts highlight a range of chromatin-associated changes at and near sites of replication stress, suggesting defects in epigenome maintenance as a potential outcome of aberrant DNA replication. Here, we will summarize recent insight into replication stress-induced chromatin-reorganization and will speculate on possible adverse effects for gene expression, nuclear integrity and, ultimately, cell function. PMID:26378584

  7. Striated fibers in trichomonads: costa proteins represent a new class of proteins forming striated roots.

    PubMed

    Viscogliosi, E; Brugerolle, G

    1994-01-01

    The production of monoclonal antibodies and the use of biochemical techniques revealed that B-type costa proteins in trichomonads are composed of several major polypeptides with molecular weight detected between 100 and 135 kDa similar to those found in the A-type costae. Although differences were observed between the two types in their fine structure, we tested whether proteins composing the two costa types belong to the same protein family. A polyclonal antibody produced against the 118 kDa costa protein of Trichomonas vaginalis also recognized a 118 kDa costa protein in all other trichomonad genera studied so far whether they have A- or B-type costae. Moreover biochemical characteristics of costa proteins indicated that these proteins might represent a novel class of striated root-forming proteins in addition to centrin, giardin, and assemblin.

  8. A large shRNA library approach identifies lncRNA Ntep as an essential regulator of cell proliferation

    PubMed Central

    Beermann, Julia; Kirste, Dominique; Iwanov, Katharina; Lu, Dongchao; Kleemiß, Felix; Kumarswamy, Regalla; Schimmel, Katharina; Bär, Christian; Thum, Thomas

    2018-01-01

    The mammalian cell cycle is a complex and tightly controlled event. Myriads of different control mechanisms are involved in its regulation. Long non-coding RNAs (lncRNA) have emerged as important regulators of many cellular processes including cellular proliferation. However, a more global and unbiased approach to identify lncRNAs with importance for cell proliferation is missing. Here, we present a lentiviral shRNA library-based approach for functional lncRNA profiling. We validated our library approach in NIH3T3 (3T3) fibroblasts by identifying lncRNAs critically involved in cell proliferation. Using stringent selection criteria we identified lncRNA NR_015491.1 out of 3842 different RNA targets represented in our library. We termed this transcript Ntep (non-coding transcript essential for proliferation), as a bona fide lncRNA essential for cell cycle progression. Inhibition of Ntep in 3T3 and primary fibroblasts prevented normal cell growth and expression of key fibroblast markers. Mechanistically, we discovered that Ntep is important to activate P53 concomitant with increased apoptosis and cell cycle blockade in late G2/M. Our findings suggest Ntep to serve as an important regulator of fibroblast proliferation and function. In summary, our study demonstrates the applicability of an innovative shRNA library approach to identify long non-coding RNA functions in a massive parallel approach. PMID:29099486

  9. Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans

    PubMed Central

    Bohr, Tisha; Ashley, Guinevere; Eggleston, Evan; Firestone, Kyra; Bhalla, Needhi

    2016-01-01

    Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes, and is essential for proper meiotic chromosome segregation. In Caenorhabditis elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on pairing centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bona fide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint. PMID:27605049

  10. A cost-effectiveness analysis of a proactive management strategy for the Sprint Fidelis recall: a probabilistic decision analysis model.

    PubMed

    Bashir, Jamil; Cowan, Simone; Raymakers, Adam; Yamashita, Michael; Danter, Matthew; Krahn, Andrew; Lynd, Larry D

    2013-12-01

    The management of the recall is complicated by the competing risks of lead failure and complications that can occur with lead revision. Many of these patients are currently undergoing an elective generator change--an ideal time to consider lead revision. To determine the cost-effectiveness of a proactive management strategy for the Sprint Fidelis recall. We obtained detailed clinical outcomes and costing data from a retrospective analysis of 341 patients who received the Sprint Fidelis lead in British Columbia, where patients younger than 60 years were offered lead extraction when undergoing generator replacement. These population-based data were used to construct and populate a probabilistic Markov model in which a proactive management strategy was compared to a conservative strategy to determine the incremental cost per lead failure avoided. In our population, elective lead revisions were half the cost of emergent revisions and had a lower complication rate. In the model, the incremental cost-effectiveness ratio of proactive lead revision versus a recommended monitoring strategy was $12,779 per lead failure avoided. The proactive strategy resulted in 21 fewer failures per 100 patients treated and reduced the chance of an additional complication from an unexpected surgery. Cost-effectiveness analysis suggests that prospective lead revision should be considered when patients with a Sprint Fidelis lead present for pulse generator change. Elective revision of the lead is justified even when 25% of the population is operated on per year, and in some scenarios, it is both less costly and provides a better outcome. © 2013 Heart Rhythm Society Published by Heart Rhythm Society All rights reserved.

  11. Homology-dependent Gene Silencing in Paramecium

    PubMed Central

    Ruiz, Françoise; Vayssié, Laurence; Klotz, Catherine; Sperling, Linda; Madeddu, Luisa

    1998-01-01

    Microinjection at high copy number of plasmids containing only the coding region of a gene into the Paramecium somatic macronucleus led to a marked reduction in the expression of the corresponding endogenous gene(s). The silencing effect, which is stably maintained throughout vegetative growth, has been observed for all Paramecium genes examined so far: a single-copy gene (ND7), as well as members of multigene families (centrin genes and trichocyst matrix protein genes) in which all closely related paralogous genes appeared to be affected. This phenomenon may be related to posttranscriptional gene silencing in transgenic plants and quelling in Neurospora and allows the efficient creation of specific mutant phenotypes thus providing a potentially powerful tool to study gene function in Paramecium. For the two multigene families that encode proteins that coassemble to build up complex subcellular structures the analysis presented herein provides the first experimental evidence that the members of these gene families are not functionally redundant. PMID:9529389

  12. How to customize a bona fide psychotherapy for generalized anxiety disorder? A two-arms, patient blinded, ABAB crossed-therapist randomized clinical implementation trial design [IMPLEMENT 2.0].

    PubMed

    Flückiger, Christoph; Wolfer, Christine; Held, Judith; Hilpert, Peter; Rubel, Julian; Allemand, Mathias; Zinbarg, Richard E; Vîslă, Andreea

    2018-04-03

    Bona fide psychotherapy approaches are effective treatments for generalized anxiety disorder (GAD) compared to no-treatment conditions. Treatment manuals and protocols allow a relatively high degree of freedom for the way therapists implement these overall treatment packages and there is a systematic lack of knowledge on how therapists should customize these treatments. The present study experimentally examines two implementation strategies of customizing a bona fide psychotherapy approach based on a 16 session time-limited cognitive-behavioral therapy (CBT) protocol and their relation to the post-session and ultimate treatment outcomes. This trial contrasts two different implementation strategies of how to customize the in-session structure of a manual-based CBT-protocol for GAD. The patients will be randomly assigned to two implementation conditions: (1) a systematic focus on subtle changes lasting from 7 to 20 min at the check-in phase of every psychotherapy session and (2) a state-of-the-art (SOTA) check-in phase lasting several minutes mainly focused on the session goals. Potential therapist effects will be examined based on an ABAB crossed-therapist design. Treatment outcomes will be assessed at the following times: post-session outcomes, treatment outcome at post assessment and 6- as well as 12-month follow-up. The proposed randomized clinical implementation trial addresses the clinically relevant question of how to customize a bona fide psychotherapy protocol experimentally contrasting two implementation strategies. Through the development and testing of the proposed implementation design, this trial has the potential to inform therapists about efficacious implementation strategies of how to customize a manual-based treatment protocol in respect to the timing of the in-session structure. This trial was registered at ClinicalTrials.gov ( NCT03079336 ) at March 14, 2017.

  13. Ion Permeation and Mechanotransduction Mechanisms of Mechanosensitive Piezo Channels.

    PubMed

    Zhao, Qiancheng; Wu, Kun; Geng, Jie; Chi, Shaopeng; Wang, Yanfeng; Zhi, Peng; Zhang, Mingmin; Xiao, Bailong

    2016-03-16

    Piezo proteins have been proposed as the long-sought-after mechanosensitive cation channels in mammals that play critical roles in various mechanotransduction processes. However, the molecular bases that underlie their ion permeation and mechanotransduction have remained functionally undefined. Here we report our finding of the miniature pore-forming module of Piezo1 that resembles the pore architecture of other trimeric channels and encodes the essential pore properties. We further identified specific residues within the pore module that determine unitary conductance, pore blockage and ion selectivity for divalent and monovalent cations and anions. The non-pore-containing region of Piezo1 confers mechanosensitivity to mechano-insensitive trimeric acid-sensing ion channels, demonstrating that Piezo1 channels possess intrinsic mechanotransduction modules separate from their pore modules. In conclusion, this is the first report on the bona fide pore module and mechanotransduction components of Piezo channels, which define their ion-conducting properties and gating by mechanical stimuli, respectively. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. Myxoma virus M130R is a novel virulence factor required for lethal myxomatosis in rabbits.

    PubMed

    Barrett, John W; Werden, Steven J; Wang, Fuan; McKillop, William M; Jimenez, June; Villeneuve, Danielle; McFadden, Grant; Dekaban, Gregory A

    2009-09-01

    Myxoma virus (MV) is a highly lethal, rabbit-specific poxvirus that induces a disease called myxomatosis in European rabbits. In an effort to understand the function of predicted immunomodulatory genes we have deleted various viral genes from MV and tested the ability of these knockout viruses to induce lethal myxomatosis. MV encodes a unique 15 kD cytoplasmic protein (M130R) that is expressed late (12h post infection) during infection. M130R is a non-essential gene for MV replication in rabbit, monkey or human cell lines. Construction of a targeted gene knockout virus (vMyx130KO) and infection of susceptible rabbits demonstrate that the M130R knockout virus is attenuated and that loss of M130R expression allows the rabbit host immune system to effectively respond to and control the lethal effects of MV. M130R expression is a bona fide poxviral virulence factor necessary for full and lethal development of myxomatosis.

  15. Callose biosynthesis regulates symplastic trafficking during root development.

    PubMed

    Vatén, Anne; Dettmer, Jan; Wu, Shuang; Stierhof, York-Dieter; Miyashima, Shunsuke; Yadav, Shri Ram; Roberts, Christina J; Campilho, Ana; Bulone, Vincent; Lichtenberger, Raffael; Lehesranta, Satu; Mähönen, Ari Pekka; Kim, Jae-Yean; Jokitalo, Eija; Sauer, Norbert; Scheres, Ben; Nakajima, Keiji; Carlsbecker, Annelie; Gallagher, Kimberly L; Helariutta, Ykä

    2011-12-13

    Plant cells are connected through plasmodesmata (PD), membrane-lined channels that allow symplastic movement of molecules between cells. However, little is known about the role of PD-mediated signaling during plant morphogenesis. Here, we describe an Arabidopsis gene, CALS3/GSL12. Gain-of-function mutations in CALS3 result in increased accumulation of callose (β-1,3-glucan) at the PD, a decrease in PD aperture, defects in root development, and reduced intercellular trafficking. Enhancement of CALS3 expression during phloem development suppressed loss-of-function mutations in the phloem abundant callose synthase, CALS7 indicating that CALS3 is a bona fide callose synthase. CALS3 alleles allowed us to spatially and temporally control the PD aperture between plant tissues. Using this tool, we are able to show that movement of the transcription factor SHORT-ROOT and microRNA165 between the stele and the endodermis is PD dependent. Taken together, we conclude that regulated callose biosynthesis at PD is essential for cell signaling. Copyright © 2011 Elsevier Inc. All rights reserved.

  16. 36 CFR 8.3 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... livestock. (d) Detectives, watchmen, guards, and caretakers. (e) Bona fide executives or department heads...) Professional sports instructors and entertainers. (h) The following employees, when approved by the Director...

  17. The essential requirement of an animal heme peroxidase protein during the wing maturation process in Drosophila.

    PubMed

    Bailey, Dondra; Basar, Mohammed Abul; Nag, Sanjay; Bondhu, Nivedita; Teng, Shaloei; Duttaroy, Atanu

    2017-01-11

    Thus far, a handful of genes have been shown to be related to the wing maturation process in insects. A novel heme peroxidase enzyme known as curly suppressor (Cysu)(formerly CG5873), have been characterized in this report because it is involved in wing morphogenesis. Using bioinformatics tools we found that Cysu is remarkably conserved in the genus Drosophila (>95%) as well as in invertebrates (>70%), although its vertebrate orthologs show poor homology. Time-lapse imaging and histochemical analyses have confirmed that the defective wing phenotype of Cysu is not a result of any underlying cellular alterations; instead, its wings fail to expand in mature adults. The precise requirement of Cysu in wings was established by identifying a bona fide mutant of Cysu from the Bloomington Drosophila Stock Centre collection. Its requirement in the wing has also been shown by RNA knockdown of the gene. Subsequent transgenic rescue of the mutant wing phenotype with the wild-type gene confirmed the phenotype resulting from Cysu mutant. With appropriate GAL4 driver like engrailed-GAL4, the Cysu phenotype was compartmentalized, which raises a strong possibility that Cysu is not localized in the extracellular matrix (ECM); hence, Cysu is not engaged in bonding the dorsal and ventral cuticular layers. Finally, shortened lifespan of the Cysu mutant suggests it is functionally essential for other biological processes as well. Cysu, a peroxinectin-like gene, is required during the wing maturation process in Drosophila because as a heme peroxidase, Cysu is capable of utilizing H 2 O 2 , which plays an essential role in post-eclosion wing morphogenesis.

  18. Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton

    PubMed Central

    Herawati, Elisa; Kanoh, Hatsuho

    2016-01-01

    Multiciliated cells (MCCs) promote fluid flow through coordinated ciliary beating, which requires properly organized basal bodies (BBs). Airway MCCs have large numbers of BBs, which are uniformly oriented and, as we show here, align linearly. The mechanism for BB alignment is unexplored. To study this mechanism, we developed a long-term and high-resolution live-imaging system and used it to observe green fluorescent protein–centrin2–labeled BBs in cultured mouse tracheal MCCs. During MCC differentiation, the BB array adopted four stereotypical patterns, from a clustering “floret” pattern to the linear “alignment.” This alignment process was correlated with BB orientations, revealed by double immunostaining for BBs and their asymmetrically associated basal feet (BF). The BB alignment was disrupted by disturbing apical microtubules with nocodazole and by a BF-depleting Odf2 mutation. We constructed a theoretical model, which indicated that the apical cytoskeleton, acting like a viscoelastic fluid, provides a self-organizing mechanism in tracheal MCCs to align BBs linearly for mucociliary transport. PMID:27573463

  19. Rational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo.

    PubMed

    Yu, Dan; Dong, Zhiqiang; Gustafson, William Clay; Ruiz-González, Rubén; Signor, Luca; Marzocca, Fanny; Borel, Franck; Klassen, Matthew P; Makhijani, Kalpana; Royant, Antoine; Jan, Yuh-Nung; Weiss, William A; Guo, Su; Shu, Xiaokun

    2016-02-01

    Fluorescent proteins (FPs) are powerful tools for cell and molecular biology. Here based on structural analysis, a blue-shifted mutant of a recently engineered monomeric infrared fluorescent protein (mIFP) has been rationally designed. This variant, named iBlueberry, bears a single mutation that shifts both excitation and emission spectra by approximately 40 nm. Furthermore, iBlueberry is four times more photostable than mIFP, rendering it more advantageous for imaging protein dynamics. By tagging iBlueberry to centrin, it has been demonstrated that the fusion protein labels the centrosome in the developing zebrafish embryo. Together with GFP-labeled nucleus and tdTomato-labeled plasma membrane, time-lapse imaging to visualize the dynamics of centrosomes in radial glia neural progenitors in the intact zebrafish brain has been demonstrated. It is further shown that iBlueberry can be used together with mIFP in two-color protein labeling in living cells and in two-color tumor labeling in mice. © 2015 The Protein Society.

  20. 22 CFR 61.5 - Authentication procedures-Imports.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... AUDIO-VISUAL MATERIALS § 61.5 Authentication procedures—Imports. (a) Applicants seeking Department authentication of foreign produced audio-visual materials shall submit to the Department a bona fide foreign...

  1. 22 CFR 61.5 - Authentication procedures-Imports.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... AUDIO-VISUAL MATERIALS § 61.5 Authentication procedures—Imports. (a) Applicants seeking Department authentication of foreign produced audio-visual materials shall submit to the Department a bona fide foreign...

  2. 22 CFR 61.5 - Authentication procedures-Imports.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... AUDIO-VISUAL MATERIALS § 61.5 Authentication procedures—Imports. (a) Applicants seeking Department authentication of foreign produced audio-visual materials shall submit to the Department a bona fide foreign...

  3. Molecular aspects of calcium signalling at the crossroads of unikont and bikont eukaryote evolution--the ciliated protozoan Paramecium in focus.

    PubMed

    Plattner, Helmut

    2015-03-01

    The ciliated protozoan, Paramecium tetraurelia has a high basic Ca(2+) leakage rate which is counteracted mainly by export through a contractile vacuole complex, based on its V-type H(+)-ATPase activity. In addition Paramecium cells dispose of P-type Ca(2+)-ATPases, i.e. a plasmamembrane and a sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase (PMCA, SERCA). Antiporter systems are to be expected, as inferred from indirect evidence. Among the best known cytosolic Ca(2+)-binding proteins, calmodulin activates Ca(2+) influx channels in the somatic cell membrane, but inactivates Ca(2+) influx channels in cilia, where it, thus, ends ciliary reversal induced by depolarization via channels in the somatic cell membrane. Centrin inactivates Ca(2+) signals after stimulation by its high capacity/low affinity binding sites, whereas its high affinity sites regulate some other functions. Cortical Ca(2+) stores (alveolar sacs) are activated during stimulated trichocyst exocytosis and thereby mediate store-operated Ca(2+) entry (SOCE). Ca(2+) release channels (CRCs) localised to alveoli and underlying SOCE are considered as Ryanodine receptor-like proteins (RyR-LPs) which are members of a CRC family with 6 subfamilies. These also encompass genuine inositol 1,4,5-trisphosphate receptors (IP3Rs) and intermediates between the two channel types. All IP3R/RyR-type CRCs possess six carboxyterminal transmembrane domains (TMD), with a pore domain between TMD 5 and 6, endowed with a characteristic selectivity filter. There are reasons to assume a common ancestor molecule for such channels and diversification further on in evolution. The distinct distribution of specific CRCs in the different vesicles undergoing intracellular trafficking suggests constitutive formation of very locally restricted Ca(2+) signals during vesicle-vesicle interaction. In summary, essential steps of Ca(2+) signalling already occur at this level of evolution, including an unexpected multitude of CRCs. For dis-/similarities with other bikonts see "Conclusions". Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system

    PubMed Central

    Szczesny, Roman J.; Kowalska, Katarzyna; Klosowska-Kosicka, Kamila; Chlebowski, Aleksander; Owczarek, Ewelina P.; Warkocki, Zbigniew; Kulinski, Tomasz M.; Adamska, Dorota; Affek, Kamila; Jedroszkowiak, Agata; Kotrys, Anna V.; Tomecki, Rafal; Krawczyk, Pawel S.; Borowski, Lukasz S.; Dziembowski, Andrzej

    2018-01-01

    Deciphering a function of a given protein requires investigating various biological aspects. Usually, the protein of interest is expressed with a fusion tag that aids or allows subsequent analyses. Additionally, downregulation or inactivation of the studied gene enables functional studies. Development of the CRISPR/Cas9 methodology opened many possibilities but in many cases it is restricted to non-essential genes. Recombinase-dependent gene integration methods, like the Flp-In system, are very good alternatives. The system is widely used in different research areas, which calls for the existence of compatible vectors and efficient protocols that ensure straightforward DNA cloning and generation of stable cell lines. We have created and validated a robust series of 52 vectors for streamlined generation of stable mammalian cell lines using the FLP recombinase-based methodology. Using the sequence-independent DNA cloning method all constructs for a given coding-sequence can be made with just three universal PCR primers. Our collection allows tetracycline-inducible expression of proteins with various tags suitable for protein localization, FRET, bimolecular fluorescence complementation (BiFC), protein dynamics studies (FRAP), co-immunoprecipitation, the RNA tethering assay and cell sorting. Some of the vectors contain a bidirectional promoter for concomitant expression of miRNA and mRNA, so that a gene can be silenced and its product replaced by a mutated miRNA-insensitive version. Our toolkit and protocols have allowed us to create more than 500 constructs with ease. We demonstrate the efficacy of our vectors by creating stable cell lines with various tagged proteins (numatrin, fibrillarin, coilin, centrin, THOC5, PCNA). We have analysed transgene expression over time to provide a guideline for future experiments and compared the effectiveness of commonly used inducers for tetracycline-responsive promoters. As proof of concept we examined the role of the exoribonuclease XRN2 in transcription termination by RNAseq. PMID:29590189

  5. 18 CFR 366.1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... NATURAL GAS ACT BOOKS AND RECORDS Definitions and Provisions Under PUHCA 2005, the Federal Power Act and... with the bona fide intention of effecting distribution within 12 months of the specific securities so...

  6. 15 CFR 315.2 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... issued to implement it: (a) Act means the Automotive Products Trade Act of 1965 (79 Stat. 1016, 19 U.S.C... a three-wheeled vehicle) or an automotive truck tractor. (d) Bona fide motor-vehicle manufacturer...

  7. 45 CFR 233.100 - Dependent children of unemployed parents.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... reason of conduct or circumstances which result or would result in disqualification for unemployment... manpower agencies, the determination as to whether the offer was bona fide, or whether there was good cause...

  8. 45 CFR 233.100 - Dependent children of unemployed parents.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... reason of conduct or circumstances which result or would result in disqualification for unemployment... manpower agencies, the determination as to whether the offer was bona fide, or whether there was good cause...

  9. 45 CFR 233.100 - Dependent children of unemployed parents.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... reason of conduct or circumstances which result or would result in disqualification for unemployment... manpower agencies, the determination as to whether the offer was bona fide, or whether there was good cause...

  10. 45 CFR 233.100 - Dependent children of unemployed parents.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... reason of conduct or circumstances which result or would result in disqualification for unemployment... manpower agencies, the determination as to whether the offer was bona fide, or whether there was good cause...

  11. 45 CFR 233.100 - Dependent children of unemployed parents.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... reason of conduct or circumstances which result or would result in disqualification for unemployment... manpower agencies, the determination as to whether the offer was bona fide, or whether there was good cause...

  12. 32 CFR 766.4 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Marine Corps aviation facility in case of a bona fide emergency.) (See § 766.5(i).) (a) General. Subject... required for loading, en route or terminal stops. (d) Test and experimental use. Aircraft being produced...

  13. 48 CFR 809.406-4 - Period of debarment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... indicated after the DSPE reviews documentary evidence submitted by or on behalf of the contractor setting... evidence, reversal of a conviction, bona fide change of ownership or management, elimination of the cause...

  14. 29 CFR 779.402 - “Executive” and “administrative” employees defined.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... employed in the capacity of academic administrative personnel for teacher in elementary or secondary... to the performance of executive or administrative activities and still qualify as a bona fide...

  15. 29 CFR 779.402 - “Executive” and “administrative” employees defined.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... employed in the capacity of academic administrative personnel for teacher in elementary or secondary... to the performance of executive or administrative activities and still qualify as a bona fide...

  16. What Are the bona fide GSK3 Substrates?

    PubMed

    Sutherland, Calum

    2011-01-01

    Nearly 100 proteins are proposed to be substrates for GSK3, suggesting that this enzyme is a fundamental regulator of almost every process in the cell, in every tissue in the body. However, it is not certain how many of these proposed substrates are regulated by GSK3 in vivo. Clearly, the identification of the physiological functions of GSK3 will be greatly aided by the identification of its bona fide substrates, and the development of GSK3 as a therapeutic target will be highly influenced by this range of actions, hence the need to accurately establish true GSK3 substrates in cells. In this paper the evidence that proposed GSK3 substrates are likely to be physiological targets is assessed, highlighting the key cellular processes that could be modulated by GSK3 activity and inhibition.

  17. Discovery of a new Galactic bona fide luminous blue variable with Spitzer★

    NASA Astrophysics Data System (ADS)

    Gvaramadze, V. V.; Kniazev, A. Y.; Berdnikov, L. N.; Langer, N.; Grebel, E. K.; Bestenlehner, J. M.

    2014-11-01

    We report the discovery of a circular mid-infrared shell around the emission-line star Wray 16-137 using archival data of the Spitzer Space Telescope. Follow-up optical spectroscopy of Wray 16-137 with the Southern African Large Telescope revealed a rich emission spectrum typical of the classical luminous blue variables (LBVs) like P Cygni. Subsequent spectroscopic and photometric observations showed drastic changes in the spectrum and brightness during the last three years, meaning that Wray 16-137 currently undergoes an S Dor-like outburst. Namely, we found that the star has brightened by ≈1 mag in the V and Ic bands, while its spectrum became dominated by Fe II lines. Taken together, our observations unambiguously show that Wray 16-137 is a new member of the family of Galactic bona fide LBVs.

  18. Pithovirus sibericum, a new bona fide member of the "Fourth TRUC" club.

    PubMed

    Sharma, Vikas; Colson, Philippe; Chabrol, Olivier; Pontarotti, Pierre; Raoult, Didier

    2015-01-01

    Nucleocytoplasmic large DNA viruses, or representatives of the proposed order Megavirales, include giant viruses of Acanthamoeba that were discovered over the last 12 years and are bona fide microbes. Phylogenies based on a few genes conserved amongst these megaviruses and shared by microbes classified as Eukarya, Bacteria, and Archaea, allowed for delineation of a fourth monophylogenetic group or "TRUC" (Things Resisting Uncompleted Classification) composed of the Megavirales representatives. A new Megavirales member named Pithovirus sibericum was isolated from a >30,000-year-old dated Siberian permafrost sample. This virion is as large as recently described pandoraviruses but has a genome that is approximately three to four times shorter. Our objective was to update the classification of P. sibericum as a new member of the "Fourth TRUC" club. Phylogenetic trees were constructed based on four conserved ancient genes and a phyletic analysis was concurrently conducted based on the presence/absence patterns of a set of informational genes from members of Megavirales, Bacteria, Archaea, and Eukarya. Phylogenetic analyses based on the four conserved genes revealed that P. sibericum is part of the fourth TRUC composed of Megavirales members, and is closely related to the families Marseilleviridae and Ascoviridae/Iridoviridae. Additionally, hierarchical clustering delineated four branches, and showed that P. sibericum is part of this fourth TRUC. Overall, phylogenetic and phyletic analyses using informational genes clearly indicate that P. sibericum is a new bona fide member of the "Fourth TRUC" club composed of representatives of Megavirales, alongside Bacteria, Archaea, and Eukarya.

  19. 76 FR 78181 - Prohibition Against Conflicts of Interest in Certain Securitizations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-16

    ... bona fide market-making. The ABS Conflicts Proposal was published in the Federal Register on September... Barry O'Connell, Attorney Advisor, Office of Trading Practices, Division of Trading and Markets, at (202...

  20. COPII-coated membranes function as transport carriers of intracellular procollagen I

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gorur, Amita; Yuan, Lin; Kenny, Samuel J.

    The coat protein complex II (COPII) is essential for the transport of large cargo, such as 300-nm procollagen I (PC1) molecules, from the endoplasmic reticulum (ER) to the Golgi. Previous work has shown that the CUL3-KLHL12 complex increases the size of COPII vesicles at ER exit sites to more than 300 nm in diameter and accelerates the secretion of PC1. However, the role of large COPII vesicles as PC1 transport carriers was not unambiguously demonstrated. In this study, using stochastic optical reconstruction microscopy, correlated light electron microscopy, and live-cell imaging, we demonstrate the existence of mobile COPII-coated vesicles that completelymore » encapsulate the cargo PC1 and are physically separated from ER. We also developed a cell-free COPII vesicle budding reaction that reconstitutes the capture of PC1 into large COPII vesicles. This process requires COPII proteins and the GTPase activity of the COPII subunit SAR1. We conclude that large COPII vesicles are bona fide carriers of PC1.« less

  1. COPII-coated membranes function as transport carriers of intracellular procollagen I

    DOE PAGES

    Gorur, Amita; Yuan, Lin; Kenny, Samuel J.; ...

    2017-04-20

    The coat protein complex II (COPII) is essential for the transport of large cargo, such as 300-nm procollagen I (PC1) molecules, from the endoplasmic reticulum (ER) to the Golgi. Previous work has shown that the CUL3-KLHL12 complex increases the size of COPII vesicles at ER exit sites to more than 300 nm in diameter and accelerates the secretion of PC1. However, the role of large COPII vesicles as PC1 transport carriers was not unambiguously demonstrated. In this study, using stochastic optical reconstruction microscopy, correlated light electron microscopy, and live-cell imaging, we demonstrate the existence of mobile COPII-coated vesicles that completelymore » encapsulate the cargo PC1 and are physically separated from ER. We also developed a cell-free COPII vesicle budding reaction that reconstitutes the capture of PC1 into large COPII vesicles. This process requires COPII proteins and the GTPase activity of the COPII subunit SAR1. We conclude that large COPII vesicles are bona fide carriers of PC1.« less

  2. 7 CFR 250.13 - Distribution and control of donated foods.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... agency to bona fide experimental or testing agencies, or for use in workshops, or for demonstrations or...) and at least one of the following is true: (i) Such other persons are common beneficiaries with the...

  3. 7 CFR 250.13 - Distribution and control of donated foods.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... agency to bona fide experimental or testing agencies, or for use in workshops, or for demonstrations or...) and at least one of the following is true: (i) Such other persons are common beneficiaries with the...

  4. 7 CFR 250.13 - Distribution and control of donated foods.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... agency to bona fide experimental or testing agencies, or for use in workshops, or for demonstrations or...) and at least one of the following is true: (i) Such other persons are common beneficiaries with the...

  5. 7 CFR 250.13 - Distribution and control of donated foods.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... agency to bona fide experimental or testing agencies, or for use in workshops, or for demonstrations or...) and at least one of the following is true: (i) Such other persons are common beneficiaries with the...

  6. 7 CFR 250.13 - Distribution and control of donated foods.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... provided by USDA on commodity survey memoranda. The USDA commodity file cost shall be defined as the cost... agency to bona fide experimental or testing agencies, or for use in workshops, or for demonstrations or...

  7. 76 FR 72921 - Proposed CERCLA Administrative Bona Fide Prospective Purchaser Settlement; The City of Dowagiac...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... and projected future response costs concerning the ICG Castings, Inc., Dowagiac site in Dowagiac..., telephone: (312) 886-6235. Comments should reference the ICG Castings, Inc., Dowagiac site in Dowagiac...

  8. Job titles need to reflect modern times and go gender neutral.

    PubMed

    Foster, Sam

    2018-04-12

    The chance to update nursing and midwifery titles can not only help streamline the confusing array of roles, but ensure that trusts employ bona fide registrants, suggests Sam Foster, Chief Nurse, Oxford University Hospitals.

  9. Remarkable modification of light curves for shadowing effects on irregular surfaces - The case of the asteroid 37 Fides

    NASA Technical Reports Server (NTRS)

    Zappala, V.; Di Martino, M.; Scaltriti, F.; Burchi, R.; Milano, L.; Young, J. W.; Wahlgren, G.; Pavlovski, K.

    1983-01-01

    Photometric observations of the asteroid 37 Fides carried out at four observatories are reported. The data were taken at phase angles ranging from approximately 2-23 deg. A composite of the light curves obtained revealed that, considering a period of 7.33 hr, the full cycle light curve exhibits one maximum and one minimum. An increase in brightness was observed following a primary minimum, a factor that indicated the cyclical appearance and disappearance of a large topographical feature. It is concluded that the 7.33 hr period is the rotational speed of the asteroid, in contrast with a previously held 14.66 hr rotational period. The method is concluded useful for identifying rotational periods of asteroids when only partial light curve data from different sources is available, and when the projections can be checked observationally.

  10. Isolation and Characterization of a Shewanella Phage–Host System from the Gut of the Tunicate, Ciona intestinalis

    PubMed Central

    Leigh, Brittany; Karrer, Charlotte; Cannon, John P.; Breitbart, Mya; Dishaw, Larry J.

    2017-01-01

    Outnumbering all other biological entities on earth, bacteriophages (phages) play critical roles in structuring microbial communities through bacterial infection and subsequent lysis, as well as through horizontal gene transfer. While numerous studies have examined the effects of phages on free-living bacterial cells, much less is known regarding the role of phage infection in host-associated biofilms, which help to stabilize adherent microbial communities. Here we report the cultivation and characterization of a novel strain of Shewanella fidelis from the gut of the marine tunicate Ciona intestinalis, inducible prophages from the S. fidelis genome, and a strain-specific lytic phage recovered from surrounding seawater. In vitro biofilm assays demonstrated that lytic phage infection affects biofilm formation in a process likely influenced by the accumulation and integration of the extracellular DNA released during cell lysis, similar to the mechanism that has been previously shown for prophage induction. PMID:28327522

  11. WS1: one more new Galactic bona fide luminous blue variable★

    NASA Astrophysics Data System (ADS)

    Kniazev, A. Y.; Gvaramadze, V. V.; Berdnikov, L. N.

    2015-04-01

    In this Letter, we report the results of spectroscopic and photometric monitoring of the candidate luminous blue variable (LBV) WS1, which was discovered in 2011 through the detection of a mid-infrared circular shell and follow-up optical spectroscopy of its central star. Our monitoring showed that WS1 brightened in the B, V and I bands by more than 1 mag during the last three years, while its spectrum revealed dramatic changes during the same time period, indicating that the star became much cooler. The light curve of WS1 demonstrates that the brightness of this star has reached maximum in 2013 December and then starts to decline. These findings unambiguously proved the LBV nature of WS1 and added one more member to the class of Galactic bona fide LBVs, bringing their number to sixteen (an updated census of these objects is provided).

  12. Stress-induced localization of HSPA6 (HSP70B') and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells.

    PubMed

    Khalouei, Sam; Chow, Ari M; Brown, Ian R

    2014-05-01

    The localization of yellow fluorescent protein (YFP)-tagged HSP70 proteins was employed to identify stress-sensitive sites in human neurons following temperature elevation. Stable lines of human SH-SY5Y neuronal cells were established that expressed YFP-tagged protein products of the human inducible HSP70 genes HSPA6 (HSP70B') and HSPA1A (HSP70-1). Following a brief period of thermal stress, YFP-tagged HSPA6 and HSPA1A rapidly appeared at centrioles in the cytoplasm of human neuronal cells, with HSPA6 demonstrating a more prolonged signal compared to HSPA1A. Each centriole is composed of a distal end and a proximal end, the latter linking the centriole doublet. The YFP-tagged HSP70 proteins targeted the proximal end of centrioles (identified by γ-tubulin marker) rather than the distal end (centrin marker). Centrioles play key roles in cellular polarity and migration during neuronal differentiation. The proximal end of the centriole, which is involved in centriole stabilization, may be stress-sensitive in post-mitotic, differentiating human neurons.

  13. Essential versus accessory aspects of cell death: recommendations of the NCCD 2015

    PubMed Central

    Galluzzi, L; Bravo-San Pedro, J M; Vitale, I; Aaronson, S A; Abrams, J M; Adam, D; Alnemri, E S; Altucci, L; Andrews, D; Annicchiarico-Petruzzelli, M; Baehrecke, E H; Bazan, N G; Bertrand, M J; Bianchi, K; Blagosklonny, M V; Blomgren, K; Borner, C; Bredesen, D E; Brenner, C; Campanella, M; Candi, E; Cecconi, F; Chan, F K; Chandel, N S; Cheng, E H; Chipuk, J E; Cidlowski, J A; Ciechanover, A; Dawson, T M; Dawson, V L; De Laurenzi, V; De Maria, R; Debatin, K-M; Di Daniele, N; Dixit, V M; Dynlacht, B D; El-Deiry, W S; Fimia, G M; Flavell, R A; Fulda, S; Garrido, C; Gougeon, M-L; Green, D R; Gronemeyer, H; Hajnoczky, G; Hardwick, J M; Hengartner, M O; Ichijo, H; Joseph, B; Jost, P J; Kaufmann, T; Kepp, O; Klionsky, D J; Knight, R A; Kumar, S; Lemasters, J J; Levine, B; Linkermann, A; Lipton, S A; Lockshin, R A; López-Otín, C; Lugli, E; Madeo, F; Malorni, W; Marine, J-C; Martin, S J; Martinou, J-C; Medema, J P; Meier, P; Melino, S; Mizushima, N; Moll, U; Muñoz-Pinedo, C; Nuñez, G; Oberst, A; Panaretakis, T; Penninger, J M; Peter, M E; Piacentini, M; Pinton, P; Prehn, J H; Puthalakath, H; Rabinovich, G A; Ravichandran, K S; Rizzuto, R; Rodrigues, C M; Rubinsztein, D C; Rudel, T; Shi, Y; Simon, H-U; Stockwell, B R; Szabadkai, G; Tait, S W; Tang, H L; Tavernarakis, N; Tsujimoto, Y; Vanden Berghe, T; Vandenabeele, P; Villunger, A; Wagner, E F; Walczak, H; White, E; Wood, W G; Yuan, J; Zakeri, Z; Zhivotovsky, B; Melino, G; Kroemer, G

    2015-01-01

    Cells exposed to extreme physicochemical or mechanical stimuli die in an uncontrollable manner, as a result of their immediate structural breakdown. Such an unavoidable variant of cellular demise is generally referred to as ‘accidental cell death' (ACD). In most settings, however, cell death is initiated by a genetically encoded apparatus, correlating with the fact that its course can be altered by pharmacologic or genetic interventions. ‘Regulated cell death' (RCD) can occur as part of physiologic programs or can be activated once adaptive responses to perturbations of the extracellular or intracellular microenvironment fail. The biochemical phenomena that accompany RCD may be harnessed to classify it into a few subtypes, which often (but not always) exhibit stereotyped morphologic features. Nonetheless, efficiently inhibiting the processes that are commonly thought to cause RCD, such as the activation of executioner caspases in the course of apoptosis, does not exert true cytoprotective effects in the mammalian system, but simply alters the kinetics of cellular demise as it shifts its morphologic and biochemical correlates. Conversely, bona fide cytoprotection can be achieved by inhibiting the transduction of lethal signals in the early phases of the process, when adaptive responses are still operational. Thus, the mechanisms that truly execute RCD may be less understood, less inhibitable and perhaps more homogeneous than previously thought. Here, the Nomenclature Committee on Cell Death formulates a set of recommendations to help scientists and researchers to discriminate between essential and accessory aspects of cell death. PMID:25236395

  14. Essential versus accessory aspects of cell death: recommendations of the NCCD 2015.

    PubMed

    Galluzzi, L; Bravo-San Pedro, J M; Vitale, I; Aaronson, S A; Abrams, J M; Adam, D; Alnemri, E S; Altucci, L; Andrews, D; Annicchiarico-Petruzzelli, M; Baehrecke, E H; Bazan, N G; Bertrand, M J; Bianchi, K; Blagosklonny, M V; Blomgren, K; Borner, C; Bredesen, D E; Brenner, C; Campanella, M; Candi, E; Cecconi, F; Chan, F K; Chandel, N S; Cheng, E H; Chipuk, J E; Cidlowski, J A; Ciechanover, A; Dawson, T M; Dawson, V L; De Laurenzi, V; De Maria, R; Debatin, K-M; Di Daniele, N; Dixit, V M; Dynlacht, B D; El-Deiry, W S; Fimia, G M; Flavell, R A; Fulda, S; Garrido, C; Gougeon, M-L; Green, D R; Gronemeyer, H; Hajnoczky, G; Hardwick, J M; Hengartner, M O; Ichijo, H; Joseph, B; Jost, P J; Kaufmann, T; Kepp, O; Klionsky, D J; Knight, R A; Kumar, S; Lemasters, J J; Levine, B; Linkermann, A; Lipton, S A; Lockshin, R A; López-Otín, C; Lugli, E; Madeo, F; Malorni, W; Marine, J-C; Martin, S J; Martinou, J-C; Medema, J P; Meier, P; Melino, S; Mizushima, N; Moll, U; Muñoz-Pinedo, C; Nuñez, G; Oberst, A; Panaretakis, T; Penninger, J M; Peter, M E; Piacentini, M; Pinton, P; Prehn, J H; Puthalakath, H; Rabinovich, G A; Ravichandran, K S; Rizzuto, R; Rodrigues, C M; Rubinsztein, D C; Rudel, T; Shi, Y; Simon, H-U; Stockwell, B R; Szabadkai, G; Tait, S W; Tang, H L; Tavernarakis, N; Tsujimoto, Y; Vanden Berghe, T; Vandenabeele, P; Villunger, A; Wagner, E F; Walczak, H; White, E; Wood, W G; Yuan, J; Zakeri, Z; Zhivotovsky, B; Melino, G; Kroemer, G

    2015-01-01

    Cells exposed to extreme physicochemical or mechanical stimuli die in an uncontrollable manner, as a result of their immediate structural breakdown. Such an unavoidable variant of cellular demise is generally referred to as 'accidental cell death' (ACD). In most settings, however, cell death is initiated by a genetically encoded apparatus, correlating with the fact that its course can be altered by pharmacologic or genetic interventions. 'Regulated cell death' (RCD) can occur as part of physiologic programs or can be activated once adaptive responses to perturbations of the extracellular or intracellular microenvironment fail. The biochemical phenomena that accompany RCD may be harnessed to classify it into a few subtypes, which often (but not always) exhibit stereotyped morphologic features. Nonetheless, efficiently inhibiting the processes that are commonly thought to cause RCD, such as the activation of executioner caspases in the course of apoptosis, does not exert true cytoprotective effects in the mammalian system, but simply alters the kinetics of cellular demise as it shifts its morphologic and biochemical correlates. Conversely, bona fide cytoprotection can be achieved by inhibiting the transduction of lethal signals in the early phases of the process, when adaptive responses are still operational. Thus, the mechanisms that truly execute RCD may be less understood, less inhibitable and perhaps more homogeneous than previously thought. Here, the Nomenclature Committee on Cell Death formulates a set of recommendations to help scientists and researchers to discriminate between essential and accessory aspects of cell death.

  15. The yeast Hot1 transcription factor is critical for activating a single target gene, STL1

    PubMed Central

    Bai, Chen; Tesker, Masha; Engelberg, David

    2015-01-01

    Transcription factors are commonly activated by signal transduction cascades and induce expression of many genes. They therefore play critical roles in determining the cell's fate. The yeast Hog1 MAP kinase pathway is believed to control the transcription of hundreds of genes via several transcription factors. To identify the bona fide target genes of Hog1, we inducibly expressed the spontaneously active variant Hog1D170A+F318L in cells lacking the Hog1 activator Pbs2. This system allowed monitoring the effects of Hog1 by itself. Expression of Hog1D170A+F318L in pbs2∆ cells imposed induction of just 105 and suppression of only 26 transcripts by at least twofold. We looked for the Hog1-responsive element within the promoter of the most highly induced gene, STL1 (88-fold). A novel Hog1 responsive element (HoRE) was identified and shown to be the direct target of the transcription factor Hot1. Unexpectedly, we could not find this HoRE in any other yeast promoter. In addition, the only gene whose expression was abolished in hot1∆ cells was STL1. Thus Hot1 is essential for transcription of just one gene, STL1. Hot1 may represent a class of transcription factors that are essential for transcription of a very few genes or even just one. PMID:25904326

  16. 29 CFR 1625.8 - Bona fide seniority systems.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN... equitable allocation of available employment opportunities and prerogatives among younger and older workers... systems which segregate, classify, or otherwise discriminate against individuals on the basis of race...

  17. 29 CFR 1625.8 - Bona fide seniority systems.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN... equitable allocation of available employment opportunities and prerogatives among younger and older workers... systems which segregate, classify, or otherwise discriminate against individuals on the basis of race...

  18. 29 CFR 1625.8 - Bona fide seniority systems.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN... equitable allocation of available employment opportunities and prerogatives among younger and older workers... systems which segregate, classify, or otherwise discriminate against individuals on the basis of race...

  19. 29 CFR 1625.8 - Bona fide seniority systems.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN... equitable allocation of available employment opportunities and prerogatives among younger and older workers... systems which segregate, classify, or otherwise discriminate against individuals on the basis of race...

  20. 29 CFR 1625.8 - Bona fide seniority systems.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION AGE DISCRIMINATION IN... equitable allocation of available employment opportunities and prerogatives among younger and older workers... systems which segregate, classify, or otherwise discriminate against individuals on the basis of race...

  1. 50 CFR 216.26 - Collection of certain marine mammal parts without prior authorization.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... living marine mammal in the wild may be collected or imported for bona fide scientific research and... must also state the scientific research or enhancement purpose for which the part was collected or...

  2. 50 CFR 216.26 - Collection of certain marine mammal parts without prior authorization.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... living marine mammal in the wild may be collected or imported for bona fide scientific research and... must also state the scientific research or enhancement purpose for which the part was collected or...

  3. Guidance Documents and Sample Forms for Central Data Exchange (CDX) Registration and e-PMN Software (Thin Client)

    EPA Pesticide Factsheets

    For users submitting electronic notices through CDX, this page contains guidance documents and sample forms to help you, submit Premanufacture Notices (PMN), Bona Fide and biotechnology notices, among others.

  4. 37 CFR 385.12 - Calculation of royalty payments in general.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... accounting period under its agreements with performing rights societies as defined in 17 U.S.C. 101, it will... information due to bona fide limitations of the available technology for services of that nature or of devices...

  5. 37 CFR 385.12 - Calculation of royalty payments in general.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... accounting period under its agreements with performing rights societies as defined in 17 U.S.C. 101, it will... information due to bona fide limitations of the available technology for services of that nature or of devices...

  6. 8 CFR 214.4 - Denial of certification, denial of recertification or withdrawal of SEVP certification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...). (xii) Failure to operate as a bona fide institution of learning. (xiii) Failure to employ adequate...) Failure to maintain the physical plant, curriculum, and teaching staff in the manner represented in the...

  7. 27 CFR 40.22 - Determination of sale price of large cigars.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., CIGARETTE PAPERS AND TUBES, AND PROCESSED TOBACCO Taxes § 40.22 Determination of sale price of large cigars... packaging. If different bona fide sale prices are applicable to different types of packaging (e. g., boxes...

  8. 27 CFR 40.22 - Determination of sale price of large cigars.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., CIGARETTE PAPERS AND TUBES, AND PROCESSED TOBACCO Taxes § 40.22 Determination of sale price of large cigars... packaging. If different bona fide sale prices are applicable to different types of packaging (e. g., boxes...

  9. 27 CFR 40.22 - Determination of sale price of large cigars.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., CIGARETTE PAPERS AND TUBES, AND PROCESSED TOBACCO Taxes § 40.22 Determination of sale price of large cigars... packaging. If different bona fide sale prices are applicable to different types of packaging (e. g., boxes...

  10. 27 CFR 40.22 - Determination of sale price of large cigars.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., CIGARETTE PAPERS AND TUBES, AND PROCESSED TOBACCO Taxes § 40.22 Determination of sale price of large cigars... packaging. If different bona fide sale prices are applicable to different types of packaging (e. g., boxes...

  11. 26 CFR 1.409A-0 - Table of contents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... (ii) Participation by nonresident aliens, certain resident aliens, and bona fide residents of.... (iii) Tax equalization agreements. (iv) Certain limited deferrals of a nonresident alien. (v...) Alternative methods of compliance. (7) Nonresident alien employees. (8) Elections affecting the identification...

  12. 26 CFR 1.409A-0 - Table of contents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... (ii) Participation by nonresident aliens, certain resident aliens, and bona fide residents of.... (iii) Tax equalization agreements. (iv) Certain limited deferrals of a nonresident alien. (v...) Alternative methods of compliance. (7) Nonresident alien employees. (8) Elections affecting the identification...

  13. 26 CFR 1.409A-0 - Table of contents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... (ii) Participation by nonresident aliens, certain resident aliens, and bona fide residents of.... (iii) Tax equalization agreements. (iv) Certain limited deferrals of a nonresident alien. (v...) Alternative methods of compliance. (7) Nonresident alien employees. (8) Elections affecting the identification...

  14. 26 CFR 1.409A-0 - Table of contents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... (ii) Participation by nonresident aliens, certain resident aliens, and bona fide residents of.... (iii) Tax equalization agreements. (iv) Certain limited deferrals of a nonresident alien. (v...) Alternative methods of compliance. (7) Nonresident alien employees. (8) Elections affecting the identification...

  15. 20 CFR 617.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR TRADE ADJUSTMENT ASSISTANCE FOR... unemployment insurance law. (i) Bona fide application for training means an individual's signed and dated application for training filed with the State agency administering the TAA training program, on a form...

  16. 20 CFR 617.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR TRADE ADJUSTMENT ASSISTANCE FOR... unemployment insurance law. (i) Bona fide application for training means an individual's signed and dated application for training filed with the State agency administering the TAA training program, on a form...

  17. 20 CFR 617.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR TRADE ADJUSTMENT ASSISTANCE FOR... unemployment insurance law. (i) Bona fide application for training means an individual's signed and dated application for training filed with the State agency administering the TAA training program, on a form...

  18. 32 CFR 728.44 - Members of security assistance training programs, foreign military sales, and their ITO...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., except bona fide emergency situations which might threaten the life or health of an individual. Generally... patient is an ITO authorized dependent), grade or rate, country of origin, diagnosis, type of elective...

  19. 32 CFR 169a.9 - Reviews: Existing in-house commercial activities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Component will place up to three notices of the requirement in the Commerce Business Daily (CBD) over a 90...) When a bona fide urgent requirement occurs, the publication period in the CBD may be reduced to two...

  20. 78 FR 20642 - Notice of Proposed CERCLA Agreement and Order on Consent for Removal Action by Bona Fide...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-05

    ... Fernando Valley Area 2 Superfund Site, Glendale Chromium Operable Unit, 264 W. Spazier Ave., Burbank... Site, Glendale Chromium Operable Unit, located at 264 W. Spazier Ave., Burbank, California. Under the...

  1. 40 CFR 5.540 - Advertising.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex... discrimination based on sex unless sex is a bona fide occupational qualification for the particular job in...

  2. 29 CFR 36.540 - Advertising.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or... indicate preference, limitation, specification, or discrimination based on sex unless sex is a bona fide...

  3. 29 CFR 36.540 - Advertising.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Secretary of Labor NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or... indicate preference, limitation, specification, or discrimination based on sex unless sex is a bona fide...

  4. 12 CFR 220.6 - Good faith account.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... may effect or finance customer transactions in accordance with the following provisions: (a... (the Supplement). (3) Satisfaction of margin. Required margin may be satisfied by a transfer from the.... (b) Arbitrage. A creditor may effect and finance for any customer bona fide arbitrage transactions...

  5. Guidelines on Discrimination Because of Sex.

    ERIC Educational Resources Information Center

    Equal Employment Opportunity Commission, Washington, DC.

    This brochure presents regulatory guidelines on sex discrimination with sections devoted to: general principles; sex as a bona fide occupational qualification; separate lines of progression and seniority systems; discrimination against married women; advertising job opportunities; employment agencies; pre-employment inquiries as to sex; the…

  6. 12 CFR 1024.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... interest or franchise relationship. (i) In an affiliated business arrangement: (A) Bona fide dividends, and capital or equity distributions, related to ownership interest or franchise relationship, between entities... corporate or partnership organizational document or a franchise agreement, will determine whether it is a...

  7. 12 CFR 1024.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... interest or franchise relationship. (i) In an affiliated business arrangement: (A) Bona fide dividends, and capital or equity distributions, related to ownership interest or franchise relationship, between entities... corporate or partnership organizational document or a franchise agreement, will determine whether it is a...

  8. 29 CFR 520.502 - What information must an application to employ student-learners at subminimum wages contain?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... accredited school, college, or university and will be employed pursuant to a bona fide vocational training...), (h) and (i) were approved by the Office of Management and Budget under control number 1215-0192.) ...

  9. 48 CFR 3.401 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... for the purpose of securing business, that neither exerts nor proposes to exert improper influence to... contract or contracts through improper influence. Bona fide employee, means a person, employed by a... performance, who neither exerts nor proposes to exert improper influence to solicit or obtain Government...

  10. Conducting Mathematical Research with Undergraduates

    ERIC Educational Resources Information Center

    Roberts, Gareth E.

    2013-01-01

    The notion that undergraduates are capable of making profound and original contributions to mathematical research is rapidly gaining acceptance. Undergraduates bring their enthusiasm, creativity, curiosity, and perseverance to bona fide research problems. This article discusses some of the key issues concerning undergraduate mathematical research:…

  11. 12 CFR 390.461 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... and upon a new, bona fide credit analysis utilizing current information on financial condition and... means a financial institution that engages in the business of banking; that is recognized as a bank by.... Direct credit substitutes include: (1) Financial standby letters of credit that support financial claims...

  12. 12 CFR 390.461 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... and upon a new, bona fide credit analysis utilizing current information on financial condition and... means a financial institution that engages in the business of banking; that is recognized as a bank by.... Direct credit substitutes include: (1) Financial standby letters of credit that support financial claims...

  13. 12 CFR 390.461 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and upon a new, bona fide credit analysis utilizing current information on financial condition and... means a financial institution that engages in the business of banking; that is recognized as a bank by.... Direct credit substitutes include: (1) Financial standby letters of credit that support financial claims...

  14. 17 CFR 200.735-5 - Securities transactions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Service position in the Division of Investment Management or the Office of Compliance Inspections and... the Division of Investment Management and the Office of Compliance Inspections and Examinations, in... cause to be effected any transaction in a security except for bona fide investment purposes. Therefore...

  15. 48 CFR 52.249-1 - Termination for Convenience of the Government (Fixed-Price) (Short Form).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., may terminate this contract, in whole or in part, when it is in the Government's interest. If this..., except for property that the Contractor (a) disposed of by bona fide sale or (b) removed from the site...

  16. 26 CFR 1.911-7 - Procedural rules.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... effective; (v) The exclusion or exclusions the individual is electing; (vi) The foreign country or countries...) The status (either bona fide residence or physical presence) under which the individual claims the exclusion; (viii) The individual's qualifying period of residence or presence; (ix) The individual's foreign...

  17. 7 CFR 27.100 - Administration.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Administration. 27.100 Section 27.100 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Administration. The details of the method of carrying out the provisions of this subpart in each bona fide spot...

  18. 7 CFR 27.100 - Administration.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Administration. 27.100 Section 27.100 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Administration. The details of the method of carrying out the provisions of this subpart in each bona fide spot...

  19. 7 CFR 27.100 - Administration.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Administration. 27.100 Section 27.100 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... Administration. The details of the method of carrying out the provisions of this subpart in each bona fide spot...

  20. BANYAN. XII. New Members of Nearby Young Associations from GAIA–Tycho Data

    NASA Astrophysics Data System (ADS)

    Gagné, Jonathan; Roy-Loubier, Olivier; Faherty, Jacqueline K.; Doyon, René; Malo, Lison

    2018-06-01

    We present a search for stellar members of young associations within 150 pc of the Sun, based on TGAS and an updated version of the BANYAN Σ software to determine Bayesian membership probabilities that includes Gaia–2MASS color-magnitude diagrams. We identify 32 new F0–M3-type bona fide members of the 10–200 Myr old Sco-Cen, Carina, Tucana-Horologium, Columba, and Octans associations and the AB Doradus, β Pictoris, and Carina-Near moving groups. These new bona fide members have measurements of their full kinematics and literature data consistent with a young age. We also confirm the membership of 66 previously known candidate members using their Gaia–Tycho trigonometric distances or new literature radial velocities, and identify 219 additional new candidate members, most of which do not yet have a radial velocity measurement. This work is the first step toward a completeness-corrected survey of young association members based on Gaia DR2 in the near future.

  1. Restricted intra-embryonic origin of bona fide hematopoietic stem cells in the chicken

    PubMed Central

    Yvernogeau, Laurent

    2017-01-01

    Hematopoietic stem cells (HSCs), which are responsible for blood cell production, are generated during embryonic development. Human and chicken embryos share features that position the chicken as a reliable and accessible alternative model to study developmental hematopoiesis. However, the existence of HSCs has never been formally proven in chicken embryos. Here, we have established a complete cartography and quantification of hematopoietic cells in the aorta during development. We demonstrate the existence of bona fide HSCs, originating from the chicken embryo aorta (and not the yolk sac, allantois or head), through an in vivo transplantation assay. Embryos transplanted in ovo with GFP embryonic tissues on the chorio-allantoic membrane provided multilineage reconstitution in adulthood. Historically, most breakthrough discoveries in the field of developmental hematopoiesis were first made in birds and later extended to mammals. Our study sheds new light on the avian model as a valuable system to study HSC production and regulation in vivo. PMID:28526756

  2. Getting the Message? Native Reactive Electrophiles Pass Two Out of Three Thresholds to be Bona Fide Signaling Mediators.

    PubMed

    Poganik, Jesse R; Long, Marcus J C; Aye, Yimon

    2018-05-01

    Precision cell signaling activities of reactive electrophilic species (RES) are arguably among the most poorly-understood means to transmit biological messages. Latest research implicates native RES to be a chemically-distinct subset of endogenous redox signals that influence cell decision making through non-enzyme-assisted modifications of specific proteins. Yet, fundamental questions remain regarding the role of RES as bona fide second messengers. Here, we lay out three sets of criteria we feel need to be met for RES to be considered as true cellular signals that directly mediate information transfer by modifying "first-responding" sensor proteins. We critically assess the available evidence and define the extent to which each criterion has been fulfilled. Finally, we offer some ideas on the future trajectories of the electrophile signaling field taking inspiration from work that has been done to understand canonical signaling mediators. Also see the video abstract here: https://youtu.be/rG7o0clVP0c. © 2018 WILEY Periodicals, Inc.

  3. Direct Reading of Bona Fide Barcode Assays for Diagnostics with Smartphone Apps.

    PubMed

    Wong, Jessica X H; Li, Xiaochun; Liu, Frank S F; Yu, Hua-Zhong

    2015-06-30

    The desire to develop new point-of-care (POC) diagnostic tools has led to the adaptation of smartphones to tackle limitations in state-of-the-art instrumentation and centralized laboratory facilities. Today's smartphones possess the computer-like ability to image and process data using mobile apps; barcode scanners are one such type of apps. We demonstrate herein that a diagnostic assay can be performed by patterning immunoassay strips in a bona fide barcode format such that after target binding and signal enhancement, the linear barcode can be read directly with a standard smartphone app. Quantitative analysis can then be performed based on the grayscale intensities with a customized mobile app. This novel diagnostic concept has been validated for a real-world application, i.e., the detection of human chorionic gonadotropin, a pregnancy hormone. With the possibility of multiplex detection, the barcode assay protocol promises to boost POC diagnosis research by the direct adaptation of mobile devices and apps.

  4. Direct Reading of Bona Fide Barcode Assays for Diagnostics with Smartphone Apps

    PubMed Central

    Wong, Jessica X. H.; Li, Xiaochun; Liu, Frank S. F.; Yu, Hua-Zhong

    2015-01-01

    The desire to develop new point-of-care (POC) diagnostic tools has led to the adaptation of smartphones to tackle limitations in state-of-the-art instrumentation and centralized laboratory facilities. Today’s smartphones possess the computer-like ability to image and process data using mobile apps; barcode scanners are one such type of apps. We demonstrate herein that a diagnostic assay can be performed by patterning immunoassay strips in a bona fide barcode format such that after target binding and signal enhancement, the linear barcode can be read directly with a standard smartphone app. Quantitative analysis can then be performed based on the grayscale intensities with a customized mobile app. This novel diagnostic concept has been validated for a real-world application, i.e., the detection of human chorionic gonadotropin, a pregnancy hormone. With the possibility of multiplex detection, the barcode assay protocol promises to boost POC diagnosis research by the direct adaptation of mobile devices and apps. PMID:26122608

  5. Direct Reading of Bona Fide Barcode Assays for Diagnostics with Smartphone Apps

    NASA Astrophysics Data System (ADS)

    Wong, Jessica X. H.; Li, Xiaochun; Liu, Frank S. F.; Yu, Hua-Zhong

    2015-06-01

    The desire to develop new point-of-care (POC) diagnostic tools has led to the adaptation of smartphones to tackle limitations in state-of-the-art instrumentation and centralized laboratory facilities. Today’s smartphones possess the computer-like ability to image and process data using mobile apps; barcode scanners are one such type of apps. We demonstrate herein that a diagnostic assay can be performed by patterning immunoassay strips in a bona fide barcode format such that after target binding and signal enhancement, the linear barcode can be read directly with a standard smartphone app. Quantitative analysis can then be performed based on the grayscale intensities with a customized mobile app. This novel diagnostic concept has been validated for a real-world application, i.e., the detection of human chorionic gonadotropin, a pregnancy hormone. With the possibility of multiplex detection, the barcode assay protocol promises to boost POC diagnosis research by the direct adaptation of mobile devices and apps.

  6. GPS-CCD: A Novel Computational Program for the Prediction of Calpain Cleavage Sites

    PubMed Central

    Gao, Xinjiao; Ma, Qian; Ren, Jian; Xue, Yu

    2011-01-01

    As one of the most essential post-translational modifications (PTMs) of proteins, proteolysis, especially calpain-mediated cleavage, plays an important role in many biological processes, including cell death/apoptosis, cytoskeletal remodeling, and the cell cycle. Experimental identification of calpain targets with bona fide cleavage sites is fundamental for dissecting the molecular mechanisms and biological roles of calpain cleavage. In contrast to time-consuming and labor-intensive experimental approaches, computational prediction of calpain cleavage sites might more cheaply and readily provide useful information for further experimental investigation. In this work, we constructed a novel software package of GPS-CCD (Calpain Cleavage Detector) for the prediction of calpain cleavage sites, with an accuracy of 89.98%, sensitivity of 60.87% and specificity of 90.07%. With this software, we annotated potential calpain cleavage sites for hundreds of calpain substrates, for which the exact cleavage sites had not been previously determined. In this regard, GPS-CCD 1.0 is considered to be a useful tool for experimentalists. The online service and local packages of GPS-CCD 1.0 were implemented in JAVA and are freely available at: http://ccd.biocuckoo.org/. PMID:21533053

  7. RNA Editing and Its Molecular Mechanism in Plant Organelles

    PubMed Central

    Ichinose, Mizuho; Sugita, Mamoru

    2016-01-01

    RNA editing by cytidine (C) to uridine (U) conversions is widespread in plant mitochondria and chloroplasts. In some plant taxa, “reverse” U-to-C editing also occurs. However, to date, no instance of RNA editing has yet been reported in green algae and the complex thalloid liverworts. RNA editing may have evolved in early land plants 450 million years ago. However, in some plant species, including the liverwort, Marchantia polymorpha, editing may have been lost during evolution. Most RNA editing events can restore the evolutionarily conserved amino acid residues in mRNAs or create translation start and stop codons. Therefore, RNA editing is an essential process to maintain genetic information at the RNA level. Individual RNA editing sites are recognized by plant-specific pentatricopeptide repeat (PPR) proteins that are encoded in the nuclear genome. These PPR proteins are characterized by repeat elements that bind specifically to RNA sequences upstream of target editing sites. In flowering plants, non-PPR proteins also participate in multiple RNA editing events as auxiliary factors. C-to-U editing can be explained by cytidine deamination. The proteins discovered to date are important factors for RNA editing but a bona fide RNA editing enzyme has yet to be identified. PMID:28025543

  8. Drosophila transcription factor Tramtrack69 binds MEP1 to recruit the chromatin remodeler NuRD.

    PubMed

    Reddy, B Ashok; Bajpe, Prashanth Kumar; Bassett, Andrew; Moshkin, Yuri M; Kozhevnikova, Elena; Bezstarosti, Karel; Demmers, Jeroen A A; Travers, Andrew A; Verrijzer, C Peter

    2010-11-01

    ATP-dependent chromatin-remodeling complexes (remodelers) are essential regulators of chromatin structure and gene transcription. How remodelers can act in a gene-selective manner has remained enigmatic. A yeast two-hybrid screen for proteins binding the Drosophila transcription factor Tramtrack69 (TTK69) identified MEP1. Proteomic characterization revealed that MEP1 is a tightly associated subunit of the NuRD remodeler, harboring the Mi2 enzymatic core ATPase. In addition, we identified the fly homolog of human Deleted in oral cancer 1 (DOC1), also known as CDK2-associated protein 1 (CDK2AP1), as a bona fide NuRD subunit. Biochemical and genetic assays supported the functional association between MEP1, Mi2, and TTK69. Genomewide expression analysis established that TTK69, MEP1, and Mi2 cooperate closely to control transcription. The TTK69 transcriptome profile correlates poorly with remodelers other than NuRD, emphasizing the selectivity of remodeler action. On the genes examined, TTK69 is able to bind chromatin in the absence of NuRD, but targeting of NuRD is dependent on TTK69. Thus, there appears to be a hierarchical relationship in which transcription factor binding precedes remodeler recruitment.

  9. 12 CFR 567.1 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... extension or renewal and upon a new, bona fide credit analysis utilizing current information on financial... means a financial institution that engages in the business of banking; that is recognized as a bank by... assumption of any credit risk is a direct credit substitute. Direct credit substitutes include: (1) Financial...

  10. 12 CFR 567.1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... extension or renewal and upon a new, bona fide credit analysis utilizing current information on financial... means a financial institution that engages in the business of banking; that is recognized as a bank by... assumption of any credit risk is a direct credit substitute. Direct credit substitutes include: (1) Financial...

  11. 12 CFR 567.1 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... extension or renewal and upon a new, bona fide credit analysis utilizing current information on financial... means a financial institution that engages in the business of banking; that is recognized as a bank by... assumption of any credit risk is a direct credit substitute. Direct credit substitutes include: (1) Financial...

  12. 12 CFR 567.1 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... extension or renewal and upon a new, bona fide credit analysis utilizing current information on financial... means a financial institution that engages in the business of banking; that is recognized as a bank by... assumption of any credit risk is a direct credit substitute. Direct credit substitutes include: (1) Financial...

  13. 38 CFR 36.4405 - Grant approval.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... sell or rent, after receiving a bona fide offer, or refuse to negotiate for the sale or rental of, or..., color, religion, sex, familial status, disability, or national origin; (ii) The eligible individual, and... housing unit relating to race, color, religion, sex, familial status, disability, or national origin is...

  14. 38 CFR 36.4405 - Grant approval.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... sell or rent, after receiving a bona fide offer, or refuse to negotiate for the sale or rental of, or..., color, religion, sex, familial status, disability, or national origin; (ii) The eligible individual, and... housing unit relating to race, color, religion, sex, familial status, disability, or national origin is...

  15. 38 CFR 36.4405 - Grant approval.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... sell or rent, after receiving a bona fide offer, or refuse to negotiate for the sale or rental of, or..., color, religion, sex, familial status, disability, or national origin; (ii) The eligible individual, and... housing unit relating to race, color, religion, sex, familial status, disability, or national origin is...

  16. 38 CFR 36.4405 - Grant approval.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... sell or rent, after receiving a bona fide offer, or refuse to negotiate for the sale or rental of, or..., color, religion, sex, familial status, disability, or national origin; (ii) The eligible individual, and... housing unit relating to race, color, religion, sex, familial status, disability, or national origin is...

  17. Nigerian Unity: In the Balance

    DTIC Science & Technology

    2013-06-01

    Religious Conflict Resolution in Nigeria. Lanham, MD: Lexington Books, 1999, pp. 70-87. Allen, Fidelis and Ufo Okeke-Uzodike. “Oil, Politics, and Conflict...Allen and Ufo Okeke-Uzodike, “Oil, Politics, and Conflict in the Niger Delta: A Nonkilling Analysis,” Africa Peace and Conflict Journal, Vol. 3, No

  18. 29 CFR 785.42 - Adjusting grievances.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Adjusting Grievances, Medical Attention, Civic and Charitable Work, and Suggestion Systems § 785.42 Adjusting grievances. Time spent in adjusting grievances between an employer and employees during the time the employees are required to be on the premises is hours worked, but in the event a bona fide union...

  19. 26 CFR 1.457-2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of section 414(j), the annual deferral for a taxable year is the present value of the increase during... value must be determined using actuarial assumptions and methods that are reasonable (both individually... paying length of service awards to bona fide volunteers (and their beneficiaries) on account of qualified...

  20. 26 CFR 1.457-2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... of section 414(j), the annual deferral for a taxable year is the present value of the increase during... value must be determined using actuarial assumptions and methods that are reasonable (both individually... paying length of service awards to bona fide volunteers (and their beneficiaries) on account of qualified...

  1. 26 CFR 1.457-2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... of section 414(j), the annual deferral for a taxable year is the present value of the increase during... value must be determined using actuarial assumptions and methods that are reasonable (both individually... paying length of service awards to bona fide volunteers (and their beneficiaries) on account of qualified...

  2. 29 CFR 785.32 - Apprenticeship training.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... spent in an organized program of related, supplemental instruction by employees working under bona fide apprenticeship programs may be excluded from working time if the following criteria are met: (a) The apprentice...) Such time does not involve productive work or performance of the apprentice's regular duties. If the...

  3. 7 CFR 27.93 - Bona fide spot markets.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., East Texas and Oklahoma, West Texas, Desert Southwest and San Joaquin Valley. Such markets will... Desert Southwest Markets and the New Mexico counties of Union, Quay, Curry, Roosevelt and Lea. Desert... counties. San Joaquin Valley All California counties except those included in the Desert Southwest market...

  4. Trustees and Toxic Waste: New Concern in the Boardroom.

    ERIC Educational Resources Information Center

    Wallach, Paul G.

    1987-01-01

    Although environmental compliance is a significant cost of college operations, very few colleges have made a bona fide effort to develop cost-effective compliance strategies. Colleges are no longer being passed over in the application of the environment laws, including the stringent new criminal provisions. (MLW)

  5. 36 CFR 20.2 - Permits; conditions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ROYALE NATIONAL PARK; COMMERCIAL FISHING § 20.2 Permits; conditions. Annual, revocable special use... commercial fishing in the waters contiguous to the Park may be granted by the Director of the National Park Service, or the Regional Director if authorized by the Director, to bona fide commercial fishermen, where...

  6. 36 CFR 290.4 - Confidentiality of cave location information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AGRICULTURE CAVE RESOURCES MANAGEMENT § 290.4 Confidentiality of cave location information. (a) Information... determines that disclosure will further the purposes of the Act and will not create a substantial risk of... Federal or State governmental agencies, bona fide educational or research institutes, or individuals or...

  7. 48 CFR 52.222-9 - Apprentices and Trainees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... fide apprenticeship program registered with the U.S. Department of Labor, Employment and Training Administration, Office of Apprenticeship Training, Employer, and Labor Services (OATELS) or with a State... work force under the registered program. (3) Any worker listed on a payroll at an apprentice wage rate...

  8. 29 CFR 516.3 - Bona fide executive, administrative, and professional employees (including academic...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... information and data required by § 516.2(a) except paragraphs (a) (6) through (10) and, in addition, the basis... appropriate addenda such as “plus hospitalization and insurance plan A,” “benefit package B,” “2 weeks paid...

  9. 29 CFR 516.3 - Bona fide executive, administrative, and professional employees (including academic...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... information and data required by § 516.2(a) except paragraphs (a) (6) through (10) and, in addition, the basis... appropriate addenda such as “plus hospitalization and insurance plan A,” “benefit package B,” “2 weeks paid...

  10. 29 CFR 516.3 - Bona fide executive, administrative, and professional employees (including academic...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... information and data required by § 516.2(a) except paragraphs (a) (6) through (10) and, in addition, the basis... appropriate addenda such as “plus hospitalization and insurance plan A,” “benefit package B,” “2 weeks paid...

  11. 29 CFR 516.3 - Bona fide executive, administrative, and professional employees (including academic...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... information and data required by § 516.2(a) except paragraphs (a) (6) through (10) and, in addition, the basis... appropriate addenda such as “plus hospitalization and insurance plan A,” “benefit package B,” “2 weeks paid...

  12. 29 CFR 516.3 - Bona fide executive, administrative, and professional employees (including academic...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... information and data required by § 516.2(a) except paragraphs (a) (6) through (10) and, in addition, the basis... appropriate addenda such as “plus hospitalization and insurance plan A,” “benefit package B,” “2 weeks paid...

  13. 7 CFR 900.101 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... General Counsel means the General Counsel of the Department; (e) The term Administrator means the..., and which is bona fide engaged in the collective processing or preparing for market or handling or marketing, in the current of interstate or foreign commerce, of milk or its products; (i) The term...

  14. When Graduate Degrees Prostitute the Educational Process: Degrees Gone Wild

    ERIC Educational Resources Information Center

    Lumadue, Richard T.

    2006-01-01

    Graduate degrees prostitute the educational process when they are sold to consumers by unaccredited degree/diploma mills as being equivalent to legitimate, bona-fide degrees awarded by accredited graduate schools. This article carefully analyzes the serious problems of bogus degrees and their association with the religious higher education…

  15. The Impossibility of Capitalist Markets in Higher Education

    ERIC Educational Resources Information Center

    Marginson, Simon

    2013-01-01

    For more than two decades, governments around the world, led by the English-speaking polities, have moved higher education systems closer to the forms of textbook economic markets. Reforms include corporatisation, competitive funding, student charges, output formats and performance reporting. But, no country has established a bona fide economic…

  16. 26 CFR 1.937-1 - Bona fide residency in a possession.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... international waters. For this purpose, a vessel is considered to be predominantly used in local and international waters if, during the taxable year, the aggregate amount of time it is used in international... individual's residence changes to the relevant possession, the individual satisfies the requirements of...

  17. 27 CFR 22.102 - Prohibited uses.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...-free alcohol. A separate charge may be made by a hospital, sanitarium or clinic for medicines... and 22.106. Hospitals may not furnish tax-free alcohol for use of physicians in their private practice....106, clinics operated for charity and not for profit may compound bona fide medicines with tax-free...

  18. 45 CFR 1614.2 - General policy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the joint venture plan must meet the following conditions: (1) The recipients involved in the joint... § 1614.6; (2) Each recipient in the joint venture must be a bona fide participant in the activities undertaken by the joint venture; and (3) The joint PAI venture must provide an opportunity for involving...

  19. 5 CFR 551.411 - Workday.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the principal activities for that day. All time spent by an employee in the performance of such... shall be considered hours of work. (c) Bona fide meal periods are not considered hours of work, except for on-duty meal periods for employees engaged in fire protection or law enforcement activities who...

  20. 5 CFR 551.411 - Workday.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... the principal activities for that day. All time spent by an employee in the performance of such... shall be considered hours of work. (c) Bona fide meal periods are not considered hours of work, except for on-duty meal periods for employees engaged in fire protection or law enforcement activities who...

  1. 42 CFR 433.54 - Bona fide donations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... health care provider; (2) Any related entity providing health care items and services; or (3) Other... behalf of individual health care providers to the State, county, or any other unit of local government... year in the case of a donation from any health care organizational entity. (e) To the extent that a...

  2. 12 CFR 563b.530 - What other requirements apply after I convert?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... quotations for the security in a recognized inter-dealer quotation system; (2) Furnishes bona fide competitive bid and offer quotations for the security on request; or (3) May effect transactions for the... Securities Dealers Automated Quotation system. (d) File all post-conversion reports that OTS requires...

  3. Coaching for ADHD

    ERIC Educational Resources Information Center

    Murphy, Kevin; Ratey, Nancy; Maynard, Sandy; Sussman, Susan; Wright, Sarah D.

    2010-01-01

    Despite limited scientific study on ADHD coaching as an intervention for adults with ADHD, the field of ADHD coaching has grown significantly and gained popularity in recent years. ADHD coaching is becoming a bona fide profession where one must advance through a rigorous training process, in order to be certified as a professional ADHD coach.…

  4. 48 CFR 52.203-5 - Covenant Against Contingent Fees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and... employed or retained to solicit or obtain this contract upon an agreement or understanding for a contingent fee, except a bona fide employee or agency. For breach or violation of this warranty, the Government...

  5. Instructional Design: Science, Technology, Both, Neither

    ERIC Educational Resources Information Center

    Gropper, George L.

    2017-01-01

    What would it take for instructional design to qualify as a bona fide applied discipline? First and foremost, a fundamental requirement is a testable and tested theoretical base. Untested rationales until verified remain in limbo. Secondly, the discipline's applied prescriptions must be demonstrably traceable to the theoretical base once it is…

  6. 31 CFR 500.552 - Research samples.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Research samples. 500.552 Section 500..., Authorizations and Statements of Licensing Policy § 500.552 Research samples. Specific licenses are issued for importation of commodities subject to § 500.204 for bona fide research purposes in sample quantities only. [40...

  7. 31 CFR 515.547 - Research samples.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 31 Money and Finance:Treasury 3 2013-07-01 2013-07-01 false Research samples. 515.547 Section 515..., Authorizations, and Statements of Licensing Policy § 515.547 Research samples. Specific licenses are issued for importation of Cuban-origin commodities for bona-fide research purposes in sample quantities only. [39 FR...

  8. 31 CFR 515.547 - Research samples.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 31 Money and Finance:Treasury 3 2014-07-01 2014-07-01 false Research samples. 515.547 Section 515..., Authorizations, and Statements of Licensing Policy § 515.547 Research samples. Specific licenses are issued for importation of Cuban-origin commodities for bona-fide research purposes in sample quantities only. [39 FR...

  9. 31 CFR 515.547 - Research samples.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Research samples. 515.547 Section 515..., Authorizations, and Statements of Licensing Policy § 515.547 Research samples. Specific licenses are issued for importation of Cuban-origin commodities for bona-fide research purposes in sample quantities only. [39 FR...

  10. 31 CFR 515.547 - Research samples.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance:Treasury 3 2012-07-01 2012-07-01 false Research samples. 515.547 Section 515..., Authorizations, and Statements of Licensing Policy § 515.547 Research samples. Specific licenses are issued for importation of Cuban-origin commodities for bona-fide research purposes in sample quantities only. [39 FR...

  11. 31 CFR 515.547 - Research samples.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Research samples. 515.547 Section 515..., Authorizations, and Statements of Licensing Policy § 515.547 Research samples. Specific licenses are issued for importation of Cuban-origin commodities for bona-fide research purposes in sample quantities only. [39 FR...

  12. 29 CFR 1604.3 - Separate lines of progression and seniority systems.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... GUIDELINES ON DISCRIMINATION BECAUSE OF SEX § 1604.3 Separate lines of progression and seniority systems. (a... lines of progression or separate seniority lists based on sex where this would adversely affect any employee unless sex is a bona fide occupational qualification for that job. Accordingly, employment...

  13. 32 CFR 766.12 - Unauthorized landings.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 5 2010-07-01 2010-07-01 false Unauthorized landings. 766.12 Section 766.12... DEPARTMENT OF THE NAVY AVIATION FACILITIES BY CIVIL AIRCRAFT § 766.12 Unauthorized landings. An aircraft that... authority, except in a bona fide emergency, is in violation of this part. Civil aircraft landing in...

  14. 26 CFR 1.988-3 - Character of exchange gain or loss.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... bona fide mistake made in good faith is on the taxpayer. (5) Independent verification—(i) Effect of... transaction (including a section 1256 contract that is also a section 988 transaction) shall be characterized... on certain identified forward contracts, futures contracts and option contracts as capital gain or...

  15. 32 CFR 732.16 - Emergency care requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MEDICAL AND DENTAL CARE Medical and Dental Care From Nonnaval Sources § 732.16 Emergency care requirements. Only in a bona fide emergency will medical, maternity, or dental services be obtained under this part... dental care. A situation where the need or apparent need for medical or dental attention does not permit...

  16. 32 CFR 732.16 - Emergency care requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... MEDICAL AND DENTAL CARE Medical and Dental Care From Nonnaval Sources § 732.16 Emergency care requirements. Only in a bona fide emergency will medical, maternity, or dental services be obtained under this part... dental care. A situation where the need or apparent need for medical or dental attention does not permit...

  17. 32 CFR 732.16 - Emergency care requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... MEDICAL AND DENTAL CARE Medical and Dental Care From Nonnaval Sources § 732.16 Emergency care requirements. Only in a bona fide emergency will medical, maternity, or dental services be obtained under this part... dental care. A situation where the need or apparent need for medical or dental attention does not permit...

  18. 32 CFR 732.16 - Emergency care requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... MEDICAL AND DENTAL CARE Medical and Dental Care From Nonnaval Sources § 732.16 Emergency care requirements. Only in a bona fide emergency will medical, maternity, or dental services be obtained under this part... dental care. A situation where the need or apparent need for medical or dental attention does not permit...

  19. 32 CFR 732.16 - Emergency care requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... MEDICAL AND DENTAL CARE Medical and Dental Care From Nonnaval Sources § 732.16 Emergency care requirements. Only in a bona fide emergency will medical, maternity, or dental services be obtained under this part... dental care. A situation where the need or apparent need for medical or dental attention does not permit...

  20. 26 CFR 1.409A-3 - Permissible payments.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... relationship, Employee G provides professional services to clients of Employer U as part of the bona fide... hardship to the service provider resulting from an illness or accident of the service provider, the service... gainful activity by reason of any medically determinable physical or mental impairment that can be...

  1. 26 CFR 1.409A-3 - Permissible payments.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... relationship, Employee G provides professional services to clients of Employer U as part of the bona fide... hardship to the service provider resulting from an illness or accident of the service provider, the service... gainful activity by reason of any medically determinable physical or mental impairment that can be...

  2. 26 CFR 1.409A-3 - Permissible payments.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... relationship, Employee G provides professional services to clients of Employer U as part of the bona fide... hardship to the service provider resulting from an illness or accident of the service provider, the service... gainful activity by reason of any medically determinable physical or mental impairment that can be...

  3. 26 CFR 1.409A-3 - Permissible payments.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... relationship, Employee G provides professional services to clients of Employer U as part of the bona fide... hardship to the service provider resulting from an illness or accident of the service provider, the service... gainful activity by reason of any medically determinable physical or mental impairment that can be...

  4. 17 CFR 242.204 - Close-out requirement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... clearing agency in any equity security that is attributable to bona fide market making activities by a registered market maker, options market maker, or other market maker obligated to quote in the over-the-counter market, the participant shall by no later than the beginning of regular trading hours on the third...

  5. 17 CFR 242.204 - Close-out requirement.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... clearing agency in any equity security that is attributable to bona fide market making activities by a registered market maker, options market maker, or other market maker obligated to quote in the over-the-counter market, the participant shall by no later than the beginning of regular trading hours on the third...

  6. 17 CFR 242.204 - Close-out requirement.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... clearing agency in any equity security that is attributable to bona fide market making activities by a registered market maker, options market maker, or other market maker obligated to quote in the over-the-counter market, the participant shall by no later than the beginning of regular trading hours on the third...

  7. 17 CFR 242.204 - Close-out requirement.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... clearing agency in any equity security that is attributable to bona fide market making activities by a registered market maker, options market maker, or other market maker obligated to quote in the over-the-counter market, the participant shall by no later than the beginning of regular trading hours on the third...

  8. 17 CFR 242.204 - Close-out requirement.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... clearing agency in any equity security that is attributable to bona fide market making activities by a registered market maker, options market maker, or other market maker obligated to quote in the over-the-counter market, the participant shall by no later than the beginning of regular trading hours on the third...

  9. 76 FR 72678 - Atlantic Highly Migratory Species; Exempted Fishing, Scientific Research, Display, and Chartering...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-25

    ... from regulation. Examples of research conducted under LOAs include tagging and releasing of sharks... researchers to collect HMS from bona fide research vessels. One example of research conducted under SRPs... require scientists to report their activities associated with these tags. Examples of research conducted...

  10. 27 CFR 53.142 - Denial, revocation or suspension of registration.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) FIREARMS MANUFACTURERS EXCISE TAXES... tax free under section 4221 of the Code in any case in which he finds that: (1) The registrant is not a bona fide manufacturer, or a purchaser reselling direct to manufacturers or exporters; (2) The...

  11. 26 CFR 48.4216(a)-2 - Exclusions from sale price.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., delivery, insurance, installation, and similar expense incurred after shipment to a customer begins, in response to the customer's order, pursuant to a bona fide sale. However, costs of such nature incurred by a... excludible. In general, unless the taxpayer establishes to the satisfaction of the district director that...

  12. 26 CFR 1.263A-1 - Uniform capitalization of costs.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... these terms and conditions, the principles of § 1.263A-7 must be applied in revaluing inventory property... tax principles. In determining whether a taxpayer is a bona-fide service provider under this paragraph... costs are marketing, selling, advertising, and distribution costs. (B) Research and experimental...

  13. 7 CFR 27.93 - Bona fide spot markets.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., East Texas and Oklahoma, West Texas, Desert Southwest and San Joaquin Valley. Such markets will... Hidalgo counties. West Texas All Texas counties not included in the East Texas, Oklahoma and Desert Southwest Markets and the New Mexico counties of Union, Quay, Curry, Roosevelt and Lea. Desert Southwest The...

  14. 7 CFR 27.93 - Bona fide spot markets.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., East Texas and Oklahoma, West Texas, Desert Southwest and San Joaquin Valley. Such markets will... Hidalgo counties. West Texas All Texas counties not included in the East Texas, Oklahoma and Desert Southwest Markets and the New Mexico counties of Union, Quay, Curry, Roosevelt and Lea. Desert Southwest The...

  15. 7 CFR 27.93 - Bona fide spot markets.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., East Texas and Oklahoma, West Texas, Desert Southwest and San Joaquin Valley. Such markets will... Hidalgo counties. West Texas All Texas counties not included in the East Texas, Oklahoma and Desert Southwest Markets and the New Mexico counties of Union, Quay, Curry, Roosevelt and Lea. Desert Southwest The...

  16. 24 CFR 3500.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... § 3500.14(g) is a return on an ownership interest or franchise relationship. (i) In an affiliated... interest or franchise relationship, between entities in an affiliate relationship, are permissible; and (B... franchise agreement, will determine whether it is a bona fide return on an ownership interest or franchise...

  17. 24 CFR 3500.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... § 3500.14(g) is a return on an ownership interest or franchise relationship. (i) In an affiliated... interest or franchise relationship, between entities in an affiliate relationship, are permissible; and (B... franchise agreement, will determine whether it is a bona fide return on an ownership interest or franchise...

  18. 24 CFR 3500.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... § 3500.14(g) is a return on an ownership interest or franchise relationship. (i) In an affiliated... interest or franchise relationship, between entities in an affiliate relationship, are permissible; and (B... franchise agreement, will determine whether it is a bona fide return on an ownership interest or franchise...

  19. 12 CFR 1024.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... § 1024.14(g) is a return on an ownership interest or franchise relationship. (i) In an affiliated... interest or franchise relationship, between entities in an affiliate relationship, are permissible; and (B... franchise agreement, will determine whether it is a bona fide return on an ownership interest or franchise...

  20. 24 CFR 3500.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... § 3500.14(g) is a return on an ownership interest or franchise relationship. (i) In an affiliated... interest or franchise relationship, between entities in an affiliate relationship, are permissible; and (B... franchise agreement, will determine whether it is a bona fide return on an ownership interest or franchise...

  1. 24 CFR 3500.15 - Affiliated business arrangements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... § 3500.14(g) is a return on an ownership interest or franchise relationship. (i) In an affiliated... interest or franchise relationship, between entities in an affiliate relationship, are permissible; and (B... franchise agreement, will determine whether it is a bona fide return on an ownership interest or franchise...

  2. 12 CFR 390.262 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... credit card loans, bona fide overdraft loans, and other loans that the State savings association has... card is any card, plate, coupon book, or other single credit device that may be used from time to time to obtain credit. Credit card account is a credit account established in conjunction with the...

  3. 12 CFR 390.262 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... credit card loans, bona fide overdraft loans, and other loans that the State savings association has... card is any card, plate, coupon book, or other single credit device that may be used from time to time to obtain credit. Credit card account is a credit account established in conjunction with the...

  4. 12 CFR 390.262 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... credit card loans, bona fide overdraft loans, and other loans that the State savings association has... card is any card, plate, coupon book, or other single credit device that may be used from time to time to obtain credit. Credit card account is a credit account established in conjunction with the...

  5. 75 FR 475 - Self-Regulatory Organizations; Financial Industry Regulatory Authority, Inc.; Notice of Filing of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-05

    ... such member's financial condition as disclosed in its most recent balance sheet prepared either in... satisfying the requirement that members make balance sheet information available to bona fide regular... most recent balance sheet . * * *'' FINRA is proposing to provide members with the option of delivering...

  6. 47 CFR 76.972 - Customer service standards.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... programmer. (c) A bona fide proposal, as used in this section, is defined as a proposal from a potential leased access programmer that includes the following information: (1) The desired length of a contract... prospective leased access programmers with the following information within three business days of the date on...

  7. 17 CFR 150.3 - Exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... this part may be exceeded to the extent such position are: (1) Bona fide hedging transactions as defined in § 1.3(z) of this chapter; (2) [Reserved] (3) Spread or arbitrage positions between single... month, in the same crop year; provided however, That such spread or arbitrage positions, when combined...

  8. Seeking Understanding by Which to Educate

    ERIC Educational Resources Information Center

    Hill, Brian V.

    2009-01-01

    In retrospect, Anselm's motto "fides quaerens intellectum" (faith seeking understanding) could fairly be taken to reflect the author's life's journey, beginning with a period of intellectual rebellion against the faith of his Methodist parents as he was entering adolescence. At the age of 14, however, in a revelatory moment, and…

  9. 29 CFR 794.110 - Activities excluded from the enterprise by the statute.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... definition quoted in § 794.106. The definition distinguishes between the related activities performed through... fide independent contractor (for example, an independent accounting or auditing firm). The latter activities are expressly excluded from the “enterprise” as defined. In addition, the definition contains a...

  10. 5 CFR 551.432 - Sleep time.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Sleep time. 551.432 Section 551.432... FAIR LABOR STANDARDS ACT Hours of Work Special Situations § 551.432 Sleep time. (a) Except as provided in paragraph (b) of this section, bona fide sleep time that fulfills the following conditions shall...

  11. 5 CFR 551.432 - Sleep time.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Sleep time. 551.432 Section 551.432... FAIR LABOR STANDARDS ACT Hours of Work Special Situations § 551.432 Sleep time. (a) Except as provided in paragraph (b) of this section, bona fide sleep time that fulfills the following conditions shall...

  12. 5 CFR 551.432 - Sleep time.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 1 2012-01-01 2012-01-01 false Sleep time. 551.432 Section 551.432... FAIR LABOR STANDARDS ACT Hours of Work Special Situations § 551.432 Sleep time. (a) Except as provided in paragraph (b) of this section, bona fide sleep time that fulfills the following conditions shall...

  13. 25 CFR 241.5 - Commercial fishing, Karluk Indian Reservation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false Commercial fishing, Karluk Indian Reservation. 241.5... FISHING IN ALASKA § 241.5 Commercial fishing, Karluk Indian Reservation. (a) Definition. The Karluk Indian... Karluk Indian Reservation shall be open to commercial fishing by bona fide native inhabitants of the...

  14. 29 CFR 541.101 - Business owner.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 3 2012-07-01 2012-07-01 false Business owner. 541.101 Section 541.101 Labor Regulations... Executive Employees § 541.101 Business owner. The term “employee employed in a bona fide executive capacity... equity interest in the enterprise in which the employee is employed, regardless of whether the business...

  15. 29 CFR 541.101 - Business owner.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 3 2011-07-01 2011-07-01 false Business owner. 541.101 Section 541.101 Labor Regulations... Executive Employees § 541.101 Business owner. The term “employee employed in a bona fide executive capacity... equity interest in the enterprise in which the employee is employed, regardless of whether the business...

  16. 29 CFR 541.101 - Business owner.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 3 2013-07-01 2013-07-01 false Business owner. 541.101 Section 541.101 Labor Regulations... Executive Employees § 541.101 Business owner. The term “employee employed in a bona fide executive capacity... equity interest in the enterprise in which the employee is employed, regardless of whether the business...

  17. 29 CFR 541.101 - Business owner.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Business owner. 541.101 Section 541.101 Labor Regulations... Executive Employees § 541.101 Business owner. The term “employee employed in a bona fide executive capacity... equity interest in the enterprise in which the employee is employed, regardless of whether the business...

  18. 26 CFR 1.409A-1 - Definitions and covered plans.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... United States is a party. (ii) Participation by nonresident aliens, certain resident aliens, and bona fide residents of possessions. With respect to an alien individual for a taxable year during which such individual is a nonresident alien, a resident alien classified as a resident alien solely under section 7701...

  19. 26 CFR 1.409A-1 - Definitions and covered plans.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... United States is a party. (ii) Participation by nonresident aliens, certain resident aliens, and bona fide residents of possessions. With respect to an alien individual for a taxable year during which such individual is a nonresident alien, a resident alien classified as a resident alien solely under section 7701...

  20. 26 CFR 1.409A-1 - Definitions and covered plans.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... United States is a party. (ii) Participation by nonresident aliens, certain resident aliens, and bona fide residents of possessions. With respect to an alien individual for a taxable year during which such individual is a nonresident alien, a resident alien classified as a resident alien solely under section 7701...

  1. 46 CFR 5.205 - Return or issuance of a credential or endorsement.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... on incompetence due to drug abuse, the deposit agreement shall provide that the credential or endorsement will not be returned until the person: (1) Successfully completes a bona fide drug abuse... drug abuse monitoring program. (c) Where the voluntary deposit is based on incompetence due to alcohol...

  2. 46 CFR 5.205 - Return or issuance of a credential or endorsement.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... on incompetence due to drug abuse, the deposit agreement shall provide that the credential or endorsement will not be returned until the person: (1) Successfully completes a bona fide drug abuse... drug abuse monitoring program. (c) Where the voluntary deposit is based on incompetence due to alcohol...

  3. 46 CFR 5.205 - Return or issuance of a credential or endorsement.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... on incompetence due to drug abuse, the deposit agreement shall provide that the credential or endorsement will not be returned until the person: (1) Successfully completes a bona fide drug abuse... drug abuse monitoring program. (c) Where the voluntary deposit is based on incompetence due to alcohol...

  4. 46 CFR 5.205 - Return or issuance of a credential or endorsement.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... on incompetence due to drug abuse, the deposit agreement shall provide that the credential or endorsement will not be returned until the person: (1) Successfully completes a bona fide drug abuse... drug abuse monitoring program. (c) Where the voluntary deposit is based on incompetence due to alcohol...

  5. 46 CFR 5.205 - Return or issuance of a credential or endorsement.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... on incompetence due to drug abuse, the deposit agreement shall provide that the credential or endorsement will not be returned until the person: (1) Successfully completes a bona fide drug abuse... drug abuse monitoring program. (c) Where the voluntary deposit is based on incompetence due to alcohol...

  6. 17 CFR 19.00 - General provisions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... and derivation of such conversion factors. (Approved by the Office of Management and Budget under... to the following: (1) Excluding products or byproducts of the cash commodity hedged. If the regular... cash positions for bona fide hedging (as defined in § 1.3(z) of this chapter), the same shall be...

  7. 26 CFR 1.410(b)-4 - Nondiscriminatory classification test.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., nature of compensation (i.e., salaried or hourly), geographic location, and similar bona fide business... (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Pension, Profit-Sharing, Stock Bonus Plans, Etc. § 1.410(b)-4 Nondiscriminatory classification test. (a) In general. A plan satisfies the nondiscriminatory classification test of...

  8. 47 CFR 25.701 - Public interest obligations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... interview; (C) Bona fide news documentary (if the appearance of the candidate is incidental to the... has become a legally qualified candidate for the office in question. (iv) Burden of proof. A candidate... questions about the contents of their political files. Provided, however, that if a requester prefers access...

  9. 12 CFR 613.3000 - Financing for farmers, ranchers, and aquatic producers or harvesters.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... owning agricultural land or engaged in the production of agricultural products, including aquatic... producers or harvesters. 613.3000 Section 613.3000 Banks and Banking FARM CREDIT ADMINISTRATION FARM CREDIT... fide farmer or rancher, or producer or harvester of aquatic products for any agricultural or aquatic...

  10. 29 CFR 541.101 - Business owner.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... equity interest in the enterprise in which the employee is employed, regardless of whether the business... 29 Labor 3 2010-07-01 2010-07-01 false Business owner. 541.101 Section 541.101 Labor Regulations... Executive Employees § 541.101 Business owner. The term “employee employed in a bona fide executive capacity...

  11. 26 CFR 1.701-2 - Anti-abuse rule.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...) INCOME TAXES (CONTINUED) Partners and Partnerships § 1.701-2 Anti-abuse rule. (a) Intent of subchapter K. Subchapter K is intended to permit taxpayers to conduct joint business (including investment) activities... subchapter K are the following requirements— (1) The partnership must be bona fide and each partnership...

  12. 26 CFR 1.701-2 - Anti-abuse rule.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...) INCOME TAXES (CONTINUED) Partners and Partnerships § 1.701-2 Anti-abuse rule. (a) Intent of subchapter K. Subchapter K is intended to permit taxpayers to conduct joint business (including investment) activities... subchapter K are the following requirements— (1) The partnership must be bona fide and each partnership...

  13. 26 CFR 1.701-2 - Anti-abuse rule.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...) INCOME TAXES (CONTINUED) Partners and Partnerships § 1.701-2 Anti-abuse rule. (a) Intent of subchapter K. Subchapter K is intended to permit taxpayers to conduct joint business (including investment) activities... subchapter K are the following requirements— (1) The partnership must be bona fide and each partnership...

  14. 36 CFR 5.4 - Commercial passenger-carrying motor vehicles.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... chartered or rented in good faith to the operator, by the owner, for general use at a charge based upon time... initiated, organized, and directed by an established bona fide school or college, institution, society or... group that arranges for the transportation, assumes responsibility for financial risk and management...

  15. Mining the Giardia genome and proteome for conserved and unique basal body proteins

    PubMed Central

    Lauwaet, Tineke; Smith, Alias J.; Reiner, David S.; Romijn, Edwin P.; Wong, Catherine C. L.; Davids, Barbara J.; Shah, Sheila A.; Yates, John R.; Gillin, Frances D.

    2015-01-01

    Giardia lamblia is a flagellated protozoan parasite and a major cause of diarrhea in humans. Its microtubular cytoskeleton mediates trophozoite motility, attachment and cytokinesis, and is characterized by an attachment disk and eight flagella that are each nucleated in a basal body. To date, only 10 giardial basal body proteins have been identified, including universal signaling proteins that are important for regulating mitosis or differentiation. In this study, we have exploited bioinformatics and proteomic approaches to identify new Giardia basal body proteins and confocal microscopy to confirm their localization in interphase trophozoites. This approach identified 75 homologs of conserved basal body proteins in the genome including 65 not previously known to be associated with Giardia basal bodies. Thirteen proteins were confirmed to co-localize with centrin to the Giardia basal bodies. We also demonstrate that most basal body proteins localize to additional cytoskeletal structures in interphase trophozoites. This might help to explain the roles of the four pairs of flagella and Giardia-specific organelles in motility and differentiation. A deeper understanding of the composition of the Giardia basal bodies will contribute insights into the complex signaling pathways that regulate its unique cytoskeleton and the biological divergence of these conserved organelles. PMID:21723868

  16. Biochemical and Genetic Analysis of the Chlamydia GroEL Chaperonins

    PubMed Central

    Illingworth, Melissa; Hooppaw, Anna J.; Ruan, Lu

    2017-01-01

    ABSTRACT Chaperonins are essential for cellular growth under normal and stressful conditions and consequently represent one of the most conserved and ancient protein classes. The paradigm Escherichia coli chaperonin, EcGroEL, and its cochaperonin, EcGroES, assist in the folding of proteins via an ATP-dependent mechanism. In addition to the presence of groEL and groES homologs, groEL paralogs are found in many bacteria, including pathogens, and have evolved poorly understood species-specific functions. Chlamydia spp., which are obligate intracellular bacteria, have reduced genomes that nonetheless contain three groEL genes, Chlamydia groEL (ChgroEL), ChgroEL2, and ChgroEL3. We hypothesized that ChGroEL is the bona fide chaperonin and that the paralogs perform novel Chlamydia-specific functions. To test our hypothesis, we investigated the biochemical properties of ChGroEL and its cochaperonin, ChGroES, and queried the in vivo essentiality of the three ChgroEL genes through targeted mutagenesis in Chlamydia trachomatis. ChGroEL hydrolyzed ATP at a rate 25% of that of EcGroEL and bound with high affinity to ChGroES, and the ChGroEL-ChGroES complex could refold malate dehydrogenase (MDH). The chlamydial ChGroEL was selective for its cognate cochaperonin, ChGroES, while EcGroEL could function with both EcGroES and ChGroES. A P35T ChGroES mutant (ChGroESP35T) reduced ChGroEL-ChGroES interactions and MDH folding activities but was tolerated by EcGroEL. Both ChGroEL-ChGroES and EcGroEL-ChGroESP35T could complement an EcGroEL-EcGroES mutant. Finally, we successfully inactivated both paralogs but not ChgroEL, leading to minor growth defects in cell culture that were not exacerbated by heat stress. Collectively, our results support novel functions for the paralogs and solidify ChGroEL as a bona fide chaperonin that is biochemically distinct from EcGroEL. IMPORTANCE Chlamydia is an important cause of human diseases, including pneumonia, sexually transmitted infections, and trachoma. The chlamydial chaperonin ChGroEL and chaperonin paralog ChGroEL2 have been associated with survival under stress conditions, and ChGroEL is linked with immunopathology elicited by chlamydial infections. However, their exact roles in bacterial survival and disease remain unclear. Our results further substantiate the hypotheses that ChGroEL is the primary chlamydial chaperonin and that the paralogs play specialized roles during infection. Furthermore, ChGroEL and the mitochondrial GroEL only functioned with their cochaperonin, in contrast to the promiscuous nature of GroEL from E. coli and Helicobacter pylori, which might indicate a divergent evolution of GroEL during the transition from a free-living organism to an obligate intracellular lifestyle. PMID:28396349

  17. Applying an Inverse Model to Estimate Ammonia Emissions at Cattle Feedlots Using Three Different Observation-Based Approaches

    NASA Astrophysics Data System (ADS)

    Shonkwiler, K. B.; Ham, J. M.; Nash, C.

    2014-12-01

    Accurately quantifying emissions of ammonia (NH3) from confined animal feeding operations (CAFOs) is vital not only to the livestock industry, but essential to understanding nitrogen cycling along the Front Range of Colorado, USA, where intensive agriculture, urban sprawl, and pristine ecosystems (e.g., Rocky Mtn Nat'l Park) lie within 100-km of each other. Most observation-based techniques for estimating NH3 emissions can be expensive and highly technical. Many methods rely on concentration observations on location, which implicitly depends on weather conditions. A system for sampling NH3 using on-site weather data was developed to allow remote measurement of NH3 in a simple, cost-effective way. These systems use passive diffusive cartridges (Radiello, Sigma-Aldrich) that provide time-averaged concentrations representative of a typical two-week deployment. Cartridge exposure is robotically managed so they are only visible when winds are 1.4 m/s or greater from the direction of the CAFO. These concentration data can be coupled with stability parameters (measured on-site) in a simple inverse model to estimate emissions (FIDES, UMR Environnement et Grandes Cultures). Few studies have directly compared emissions estimates of NH3 using concentration data obtained from multiple measurement systems at different temporal and spatial scales. Therefore, in the summer and autumn of 2014, several conditional sampler systems were deployed at a 25,000-head cattle feedlot concomitant with an open-path infrared laser (GasFinder2, Boreal Laser Inc.) and a Cavity Ring Down Spectrometer (CRDS) (G1103, Picarro Inc.) which each measured instantaneous NH3 concentrations. This study will test the sampler technology by first comparing concentration data from the three different methods. In livestock research, it is common to estimate NH3 emissions by using such instantaneous data in a backward Lagrangian stochastic (bLs) model (WindTrax, Thunder Beach Sci.) Considering this, NH3 fluxes from the inverse model (FIDES) using all three datasets will be compared to emissions from the bLS model (WindTrax) using only high speed data (laser; CRDS). Results may lend further validity to the conditional sampler approach for more easily and accurately monitoring NH3 fluxes from CAFOs and other strong areal sources.

  18. 29 CFR 778.214 - Benefit plans; including profit-sharing plans or trusts providing similar benefits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... fide plan for providing old age, retirement, life, accident, or health insurance or similar benefits for employees * * *.” Such sums may not, however, be credited toward overtime compensation due under... matching employee contributions or otherwise encourages thrift or savings. Where such a plan or trust is...

  19. 19 CFR 148.13 - Written declarations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...) When required. Unless an oral declaration is accepted under § 148.12, the declaration required of a..., HTSUS, for articles to be disposed of as bona fide gifts; or subheading 9804.00.40, HTSUS, for articles... passenger shall state either: (1) The price actually paid for the article in the currency of purchase, or...

  20. 26 CFR 26.2651-2 - Individual assigned to more than 1 generation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... facts and circumstances. The most significant factor is whether there is a bona fide parent/child... assumed all significant responsibilities for the care and raising of the adopted child. Other factors may... treated as a member of the generation that is one generation below the adoptive parent for purposes of...

  1. 26 CFR 26.2651-2 - Individual assigned to more than 1 generation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... facts and circumstances. The most significant factor is whether there is a bona fide parent/child... assumed all significant responsibilities for the care and raising of the adopted child. Other factors may... treated as a member of the generation that is one generation below the adoptive parent for purposes of...

  2. 26 CFR 26.2651-2 - Individual assigned to more than 1 generation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... facts and circumstances. The most significant factor is whether there is a bona fide parent/child... assumed all significant responsibilities for the care and raising of the adopted child. Other factors may... treated as a member of the generation that is one generation below the adoptive parent for purposes of...

  3. 26 CFR 26.2651-2 - Individual assigned to more than 1 generation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... facts and circumstances. The most significant factor is whether there is a bona fide parent/child... assumed all significant responsibilities for the care and raising of the adopted child. Other factors may... treated as a member of the generation that is one generation below the adoptive parent for purposes of...

  4. 26 CFR 26.2651-2 - Individual assigned to more than 1 generation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... facts and circumstances. The most significant factor is whether there is a bona fide parent/child... assumed all significant responsibilities for the care and raising of the adopted child. Other factors may... treated as a member of the generation that is one generation below the adoptive parent for purposes of...

  5. 38 CFR 36.4206 - Underwriting standards, occupancy, and non-discrimination requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... to sell or rent, after the making of a bona fide offer, or refuse to negotiate for the sale or rental... because of race, color, religion, sex, handicap, familial status, or national origin; (ii) The veteran recognizes that any restrictive covenant on the property relating to race, color, religion, sex, handicap...

  6. 38 CFR 36.4206 - Underwriting standards, occupancy, and non-discrimination requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... to sell or rent, after the making of a bona fide offer, or refuse to negotiate for the sale or rental... because of race, color, religion, sex, handicap, familial status, or national origin; (ii) The veteran recognizes that any restrictive covenant on the property relating to race, color, religion, sex, handicap...

  7. 38 CFR 36.4206 - Underwriting standards, occupancy, and non-discrimination requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... to sell or rent, after the making of a bona fide offer, or refuse to negotiate for the sale or rental... because of race, color, religion, sex, handicap, familial status, or national origin; (ii) The veteran recognizes that any restrictive covenant on the property relating to race, color, religion, sex, handicap...

  8. 38 CFR 36.4206 - Underwriting standards, occupancy, and non-discrimination requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... to sell or rent, after the making of a bona fide offer, or refuse to negotiate for the sale or rental... because of race, color, religion, sex, handicap, familial status, or national origin; (ii) The veteran recognizes that any restrictive covenant on the property relating to race, color, religion, sex, handicap...

  9. 38 CFR 36.4206 - Underwriting standards, occupancy, and non-discrimination requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... to sell or rent, after the making of a bona fide offer, or refuse to negotiate for the sale or rental... because of race, color, religion, sex, handicap, familial status, or national origin; (ii) The veteran recognizes that any restrictive covenant on the property relating to race, color, religion, sex, handicap...

  10. 48 CFR 52.222-6 - Davis-Bacon Act.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... or nearly so, to the performance of a contract. (b)(1) All laborers and mechanics employed or working... contractual relationship which may be alleged to exist between the Contractor and such laborers and mechanics... fide fringe benefits under section 1(b)(2) of the Davis-Bacon Act on behalf of laborers or mechanics...

  11. 48 CFR 52.222-6 - Davis-Bacon Act.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... or nearly so, to the performance of a contract. (b)(1) All laborers and mechanics employed or working... contractual relationship which may be alleged to exist between the Contractor and such laborers and mechanics... fide fringe benefits under section 1(b)(2) of the Davis-Bacon Act on behalf of laborers or mechanics...

  12. 30 CFR 1206.301 - Value basis for royalty computation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) The gross value for royalty purposes shall be the sale or contract unit price times the number of units sold, Provided, however, That where the authorized officer determines: (1) That a contract of sale... whole or in part upon considerations other than the value of the commodities, or (2) That no bona fide...

  13. 30 CFR 1206.301 - Value basis for royalty computation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) The gross value for royalty purposes shall be the sale or contract unit price times the number of units sold, Provided, however, That where the authorized officer determines: (1) That a contract of sale... whole or in part upon considerations other than the value of the commodities, or (2) That no bona fide...

  14. 30 CFR 1206.301 - Value basis for royalty computation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) The gross value for royalty purposes shall be the sale or contract unit price times the number of units sold, Provided, however, That where the authorized officer determines: (1) That a contract of sale... whole or in part upon considerations other than the value of the commodities, or (2) That no bona fide...

  15. 30 CFR 206.301 - Value basis for royalty computation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... gross value for royalty purposes shall be the sale or contract unit price times the number of units sold, Provided, however, That where the authorized officer determines: (1) That a contract of sale or other... lease is not a bona fide transaction between independent parties because it is based in whole or in part...

  16. 19 CFR 148.44 - Gifts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Gifts. 148.44 Section 148.44 Customs Duties U.S...) PERSONAL DECLARATIONS AND EXEMPTIONS Exemptions for Nonresidents § 148.44 Gifts. (a) Exemption. An arriving... and are to be disposed of by him as bona fide gifts. See § 148.43(b) for limitations on cigars under...

  17. 12 CFR 560.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...)). Consumer loans do not include credit extended in connection with credit card loans, bona fide overdraft... authority other than section 5(c)(2)(D) of the HOLA. Credit card is any card, plate, coupon book, or other single credit device that may be used from time to time to obtain credit. Credit card account is a credit...

  18. 26 CFR 1.305-7 - Certain transactions treated as distributions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... stock (or securities), or in the exercise price of rights or warrants, made pursuant to a bona fide... distributions. (a) In general. Under section 305(c), a change in conversion ratio, a change in redemption price, a difference between redemption price and issue price, a redemption which is treated as a...

  19. 21 CFR 1316.23 - Confidentiality of identity of research subjects.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 9 2010-04-01 2010-04-01 false Confidentiality of identity of research subjects... Confidentiality of identity of research subjects. (a) Any person conducting a bona fide research project directly.... 1242; 21 U.S.C. 801) who intends to maintain the confidentiality of the identity of those persons who...

  20. 47 CFR 73.9002 - Sale or distribution of demodulators, covered demodulator products, and peripheral TSP products.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... COMMUNICATIONS COMMISSION (CONTINUED) BROADCAST RADIO SERVICES RADIO BROADCAST SERVICES Digital Broadcast... following conditions is true: (i) The party is a bona fide reseller; (ii) The party is a licensed digital... engaged, or about to become engaged, in the lawful retransmission of unencrypted digital terrestrial...

  1. A bill to amend the Commodity Exchange Act to require a regulation to limit the aggregate positions of nontraditional bona fide hedgers in petroleum and related products.

    THOMAS, 112th Congress

    Sen. Pryor, Mark L. [D-AR

    2012-03-27

    Senate - 03/27/2012 Read twice and referred to the Committee on Agriculture, Nutrition, and Forestry. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  2. 17 CFR 242.203 - Borrowing and delivery requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... purchasing securities of like kind and quantity; (iii) Short sales effected by a market maker in connection with bona-fide market making activities in the security for which this exception is claimed; and (iv... § 242.200(g) existed; and (C) Either that the condition of the market at the time the mistake was...

  3. 17 CFR 242.203 - Borrowing and delivery requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... purchasing securities of like kind and quantity; (iii) Short sales effected by a market maker in connection with bona-fide market making activities in the security for which this exception is claimed; and (iv... § 242.200(g) existed; and (C) Either that the condition of the market at the time the mistake was...

  4. 17 CFR 242.203 - Borrowing and delivery requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... purchasing securities of like kind and quantity; (iii) Short sales effected by a market maker in connection with bona-fide market making activities in the security for which this exception is claimed; and (iv... § 242.200(g) existed; and (C) Either that the condition of the market at the time the mistake was...

  5. 17 CFR 242.203 - Borrowing and delivery requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... purchasing securities of like kind and quantity; (iii) Short sales effected by a market maker in connection with bona-fide market making activities in the security for which this exception is claimed; and (iv... § 242.200(g) existed; and (C) Either that the condition of the market at the time the mistake was...

  6. 17 CFR 242.203 - Borrowing and delivery requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... purchasing securities of like kind and quantity; (iii) Short sales effected by a market maker in connection with bona-fide market making activities in the security for which this exception is claimed; and (iv... § 242.200(g) existed; and (C) Either that the condition of the market at the time the mistake was...

  7. 77 FR 54633 - Self-Regulatory Organizations; BATS Exchange, Inc.; Order Approving Proposed Rule Change, as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-05

    ... quotation for display to comply with market making obligations. Compliant displayed quotations are... able to qualify as bona-fide market making for purposes of Regulation SHO, depending on the facts and... making is appropriate with respect to the particular Market Maker Peg Order and its designated offset...

  8. 26 CFR 1.937-1 - Bona fide residency in a possession.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 120 days in foreign countries. When traveling on business to State A, T often stays at his mother's... this section, T's mother's house is not a permanent home of T. Assuming that no other accommodations in... individual's tax home is located (applying the rules of paragraph (d) of this section). (4) Qualifying...

  9. 26 CFR 1.937-1 - Bona fide residency in a possession.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 120 days in foreign countries. When traveling on business to State A, T often stays at his mother's... this section, T's mother's house is not a permanent home of T. Assuming that no other accommodations in... individual's tax home is located (applying the rules of paragraph (d) of this section). (4) Qualifying...

  10. 26 CFR 1.937-1 - Bona fide residency in a possession.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 120 days in foreign countries. When traveling on business to State A, T often stays at his mother's... this section, T's mother's house is not a permanent home of T. Assuming that no other accommodations in... individual's tax home is located (applying the rules of paragraph (d) of this section). (4) Qualifying...

  11. 26 CFR 1.937-1 - Bona fide residency in a possession.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 120 days in foreign countries. When traveling on business to State A, T often stays at his mother's... this section, T's mother's house is not a permanent home of T. Assuming that no other accommodations in... individual's tax home is located (applying the rules of paragraph (d) of this section). (4) Qualifying...

  12. Truancy Reduction: Keeping Students in School. Juvenile Justice Bulletin.

    ERIC Educational Resources Information Center

    Baker, Myriam L.; Sigmon, Jane Nady; Nugent, M. Elaine

    Each school day, hundreds of thousands of students are missing from their classrooms--many without a bona fide excuse. Left unchecked, truancy is a risk factor for serious juvenile delinquency. Truancy's impact also extends into the adult years where it has been linked to numerous negative outcomes. Consequently, it is critical to identify…

  13. 75 FR 11641 - Community Reinvestment Act; Interagency Questions and Answers Regarding Community Reinvestment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-11

    ... lending test applicable to large institutions, its content may also be applicable to, for example, small... the guidance that describes the lending, investment, and service tests. The Questions and Answers are... received total quantitative CRA consideration. Although this is true if the express, bona fide intent of...

  14. 26 CFR 25.2512-8 - Transfers for insufficient consideration.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the value of the property transferred by the donor exceeds the value in money or money's worth of the... course of business (a transaction which is bona fide, at arm's length, and free from any donative intent), will be considered as made for an adequate and full consideration in money or money's worth. A...

  15. 26 CFR 25.2512-8 - Transfers for insufficient consideration.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the value of the property transferred by the donor exceeds the value in money or money's worth of the... course of business (a transaction which is bona fide, at arm's length, and free from any donative intent), will be considered as made for an adequate and full consideration in money or money's worth. A...

  16. 26 CFR 25.2512-8 - Transfers for insufficient consideration.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... the value of the property transferred by the donor exceeds the value in money or money's worth of the... course of business (a transaction which is bona fide, at arm's length, and free from any donative intent), will be considered as made for an adequate and full consideration in money or money's worth. A...

  17. 26 CFR 25.2512-8 - Transfers for insufficient consideration.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the value of the property transferred by the donor exceeds the value in money or money's worth of the... course of business (a transaction which is bona fide, at arm's length, and free from any donative intent), will be considered as made for an adequate and full consideration in money or money's worth. A...

  18. 26 CFR 25.2512-8 - Transfers for insufficient consideration.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... the value of the property transferred by the donor exceeds the value in money or money's worth of the... course of business (a transaction which is bona fide, at arm's length, and free from any donative intent), will be considered as made for an adequate and full consideration in money or money's worth. A...

  19. 12 CFR 160.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... loans do not include credit extended in connection with credit card loans, bona fide overdraft loans... authority other than section 5(c)(2)(D) of the HOLA. Credit card is any card, plate, coupon book, or other single credit device that may be used from time to time to obtain credit. Credit card account is a credit...

  20. 12 CFR 560.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...)). Consumer loans do not include credit extended in connection with credit card loans, bona fide overdraft... authority other than section 5(c)(2)(D) of the HOLA. Credit card is any card, plate, coupon book, or other single credit device that may be used from time to time to obtain credit. Credit card account is a credit...

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