Creating and Sustaining Effective Partnership between Government and Industry

DTIC Science & Technology

2011-04-30

defense industry, fielding, contracting, interoperability, organizational behavior, risk management , cost estimating, and many others. Approaches...Finance from Cameron University and an MBA from Drury University. [scott.fouse@dau.mil] Allen Green—Engineer and Program Manager , SAIC, Inc...Program Executive Officer SHIPS • Commander, Naval Sea Systems Command • Army Contracting Command, U.S. Army Materiel Command • Program Manager , Airborne

The Effect of Ionospheric Variability on the Accuracy of High Frequency Position Location

DTIC Science & Technology

1981-08-01

A~~ ~ C~ %. 0 I Cf v. >) LaJ 04) Of T C 4 S- o 0) 0.~ o~~ o V0 Li.. .- 4 U V) (0 .. c-., c X e CO m. Co S.. LC)) C a) 4.0 I~ 0.- 0- CL CJ u W 3 LL X...Field Artillery School ATTN: STEDP-MT-DA-L ATTN: ATSF- CF -R (CPT James M. Watson) Dugway, UT 84022 Fort Sill, OK 73503 Commander Commandant US Army...Commander Commandant US Army Dugway Proving Ground US Army Field Artillery School ATTN: STEDP-MT-DA-T (Mr. John Trethewey) ATTN: ATSF- CF -R Dugway, UT 84022

GLINT: a user-friendly toolset for the analysis of high-throughput DNA-methylation array data.

PubMed

Rahmani, Elior; Yedidim, Reut; Shenhav, Liat; Schweiger, Regev; Weissbrod, Omer; Zaitlen, Noah; Halperin, Eran

2017-06-15

GLINT is a user-friendly command-line toolset for fast analysis of genome-wide DNA methylation data generated using the Illumina human methylation arrays. GLINT, which does not require any programming proficiency, allows an easy execution of Epigenome-Wide Association Study analysis pipeline under different models while accounting for known confounders in methylation data. GLINT is a command-line software, freely available at https://github.com/cozygene/glint/releases . It requires Python 2.7 and several freely available Python packages. Further information and documentation as well as a quick start tutorial are available at http://glint-epigenetics.readthedocs.io . elior.rahmani@gmail.com or ehalperin@cs.ucla.edu. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

Guidelines for Managing Vegetation on Earth-Covered Magazines Within the U.S. Army Materiel Command

DTIC Science & Technology

1994-05-01

Plant Basil Kirby, Pest Controller, Letterkenny Army Depot Ken Davis, Safety Office, Depot Systt., Command Robert Klein, Army Materiel Command Field...refertilization, herbicides, tion techniques; this should be done by visiting the plant growth regulators, pesticides , mowing or site, talking to the

A Low Noise, Microprocessor-Controlled, Internally Digitizing Rotating-Vane Electric Field Mill for Airborne Platforms

NASA Technical Reports Server (NTRS)

Bateman, M. G.; Stewart, M. F.; Blakeslee, R. J.; Podgorny, s. J.; Christian, H. J.; Mach, D. M.; Bailey, J. C.; Daskar, D.

2006-01-01

This paper reports on a new generation of aircraft-based rotating-vane style electric field mills designed and built at NASA's Marshall Spaceflight Center. The mills have individual microprocessors that digitize the electric field signal at the mill and respond to commands from the data system computer. The mills are very sensitive (1 V/m per bit), have a wide dynamic range (115 dB), and are very low noise (+/-1 LSB). Mounted on an aircraft, these mills can measure fields from +/-1 V/m to +/-500 kV/m. Once-per-second commanding from the data collection computer to each mill allows for precise timing and synchronization. The mills can also be commanded to execute a self-calibration in flight, which is done periodically to monitor the status and health of each mill.

Torque shudder protection device and method

DOEpatents

King, Robert D.; De Doncker, Rik W. A. A.; Szczesny, Paul M.

1997-01-01

A torque shudder protection device for an induction machine includes a flux command generator for supplying a steady state flux command and a torque shudder detector for supplying a status including a negative status to indicate a lack of torque shudder and a positive status to indicate a presence of torque shudder. A flux adapter uses the steady state flux command and the status to supply a present flux command identical to the steady state flux command for a negative status and different from the steady state flux command for a positive status. A limiter can receive the present flux command, prevent the present flux command from exceeding a predetermined maximum flux command magnitude, and supply the present flux command to a field oriented controller. After determining a critical electrical excitation frequency at which a torque shudder occurs for the induction machine, a flux adjuster can monitor the electrical excitation frequency of the induction machine and adjust a flux command to prevent the monitored electrical excitation frequency from reaching the critical electrical excitation frequency.

Torque shudder protection device and method

DOEpatents

King, R.D.; Doncker, R.W.A.A. De.; Szczesny, P.M.

1997-03-11

A torque shudder protection device for an induction machine includes a flux command generator for supplying a steady state flux command and a torque shudder detector for supplying a status including a negative status to indicate a lack of torque shudder and a positive status to indicate a presence of torque shudder. A flux adapter uses the steady state flux command and the status to supply a present flux command identical to the steady state flux command for a negative status and different from the steady state flux command for a positive status. A limiter can receive the present flux command, prevent the present flux command from exceeding a predetermined maximum flux command magnitude, and supply the present flux command to a field oriented controller. After determining a critical electrical excitation frequency at which a torque shudder occurs for the induction machine, a flux adjuster can monitor the electrical excitation frequency of the induction machine and adjust a flux command to prevent the monitored electrical excitation frequency from reaching the critical electrical excitation frequency. 5 figs.

Fielding a Division Staff in the Modern Day

DTIC Science & Technology

2016-06-10

FIELDING A DIVISION STAFF IN THE MODERN DAY A thesis presented to the Faculty of the U.S. Army Command and General Staff ...TITLE AND SUBTITLE Fielding a Division Staff in the Modern Day 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6...ADDRESS(ES) U.S. Army Command and General Staff College ATTN: ATZL-SWD-GD Fort Leavenworth, KS 66027-2301 8. PERFORMING ORG REPORT NUMBER 9

Marine Tactical Command and Control System (MTACCS) Field Development System-1 (FDS-1) assessment: Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Avery, L.W.; Hunt, S.T.; Savage, S.F.

1992-04-01

The United State Marine Corps (USMC) is continuing the development and fielding of the Marine Corps Tactical Command and Control System (MTACCS), a system which exists in varying states of development, fielding, or modernization. MTACCS is currently composed of the following components: Tactical Combat Operations System (TCO) for ground command and control (C2), Intelligence Analysis System (IAS) with a Genser terminal connected to a TCO workstation for intelligence C2, Marine Integrated Personnel System (MIPS) and a TCO workstation using the Marine Combat Personnel System (MCPERS) software for personnel C2, Marine Integrated Logistics System (MILOGS) which is composed of the Landingmore » Force Asset Distribution System (LFADS), the Marine Air-Ground Task Force (MAGTF) II, and a TCO terminal using the Marine Combat Logistics System (MCLOG) for logistics C2, Marine Corps Fire Support System (MCFSS) for fire support C2, and Advanced Tactical Air Command Central (ATACC) and the Improved Direct Air Support Central for aviation C2.« less

Closed-loop torque feedback for a universal field-oriented controller

DOE Office of Scientific and Technical Information (OSTI.GOV)

De Doncker, R.W.A.A.; King, R.D.; Sanza, P.C.

A torque feedback system is employed in a universal field-oriented (UFO) controller to tune a torque-producing current command and a slip frequency command in order to achieve robust torque control of an induction machine even in the event of current regulator errors and during transitions between pulse width modulated (PWM) and square wave modes of operation. 1 figure.

Closed-loop torque feedback for a universal field-oriented controller

DOEpatents

De Doncker, R.W.A.A.; King, R.D.; Sanza, P.C.; Haefner, K.B.

1992-11-24

A torque feedback system is employed in a universal field-oriented (UFO) controller to tune a torque-producing current command and a slip frequency command in order to achieve robust torque control of an induction machine even in the event of current regulator errors and during transitions between pulse width modulated (PWM) and square wave modes of operation. 1 figure.

Closed-loop torque feedback for a universal field-oriented controller

DOE Office of Scientific and Technical Information (OSTI.GOV)

De Doncker, Rik W. A. A.; King, Robert D.; Sanza, Peter C.

A torque feedback system is employed in a universal field-oriented (UFO) controller to tune a torque-producing current command and a slip frequency command in order to achieve robust torque control of an induction machine even in the event of current regulator errors and during transitions between pulse width modulated (PWM) and square wave modes of operation.

The Visioceilometer: A Portable Visibility and Cloud Ceiling Height Lidar.

DTIC Science & Technology

1982-01-01

Artillery School ATTN: STEDP-MT-DA-L ATTN: ATSF- CF -R (CPT James M. Watson) Dugway, UT 84022 Fort Sill, OK 73503 Commander Commandant US Army Dugway Proving...Commandant US Army Dugway Proving Ground US Army Field Artillery School ATTN: STEDP-MT-DA-T (Mr. John Trethewey) ATTN: ATSF- CF -R Dugway, UT 84022 Fort...Base, VA 23655 Fort !elvoir, VA 22060 Commander Director US Army INSCOM/Quest Research Corporation US Army Night Vision & ATTN: Mr. Donald Wilmot

76 FR 9039 - Emergency Responder Field Operations Guide

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-16

... Command System during incident operations. DATES: Comments must be received by March 18, 2011. ADDRESSES... (ER FOG) is intended for use when implementing the Incident Command System (ICS) in response to an...

DefenseLink Feature: Travels with Gates

Science.gov Websites

| Photos Gates Seeks to Build on Positive Momentum in Iraq Defense Secretary Robert Gates meets with local build on recent momentum. Story Commanders Urge Vigilance, Say Strategy Working Field commanders

ATM-dependent pathways of chromatin remodelling and oxidative DNA damage responses.

PubMed

Berger, N Daniel; Stanley, Fintan K T; Moore, Shaun; Goodarzi, Aaron A

2017-10-05

Ataxia-telangiectasia mutated (ATM) is a serine/threonine protein kinase with a master regulatory function in the DNA damage response. In this role, ATM commands a complex biochemical network that signals the presence of oxidative DNA damage, including the dangerous DNA double-strand break, and facilitates subsequent repair. Here, we review the current state of knowledge regarding ATM-dependent chromatin remodelling and epigenomic alterations that are required to maintain genomic integrity in the presence of DNA double-strand breaks and/or oxidative stress. We will focus particularly on the roles of ATM in adjusting nucleosome spacing at sites of unresolved DNA double-strand breaks within complex chromatin environments, and the impact of ATM on preserving the health of cells within the mammalian central nervous system.This article is part of the themed issue 'Chromatin modifiers and remodellers in DNA repair and signalling'. © 2017 The Author(s).

STS-28 Columbia, OV-102, Commander Shaw aboard T-38A at Ellington Field

NASA Image and Video Library

1989-08-05

STS-28 Columbia, Orbiter Vehicle (OV) 102, Commander Brewster H. Shaw, wearing navy blue flight coveralls and helmet, sits in T-38A forward cockpit. Shaw, along with his fellow crewmembers, is preparing for departure from Ellington Field to Kennedy Space Center (KSC). STS-28, a Department of Defense (DOD) dedicated mission, is scheduled for launch on 08-08-89.

STS-28 Columbia, OV-102, Commander Shaw aboard T-38A at Ellington Field

NASA Technical Reports Server (NTRS)

1989-01-01

STS-28 Columbia, Orbiter Vehicle (OV) 102, Commander Brewster H. Shaw, wearing navy blue flight coveralls and helmet, sits in T-38A forward cockpit. Shaw, along with his fellow crewmembers, is preparing for departure from Ellington Field to Kennedy Space Center (KSC). STS-28, a Department of Defense (DOD) dedicated mission, is scheduled for launch on 08-08-89.

Predicting Military Recruiter Effectiveness: A Literature Review

DTIC Science & Technology

1987-04-01

employing commanding officer nominations and/or supervisor ratings as criteria for success in recruiting. Wollack and KiDnis (1960). Commanding officer...ratings can be used to predict field recruiter performance. The authors attribute the failure to predict field recruiter performance to the...Time to Complete -12 -27 -5 -09 5. MC 431 Completion/ Failure 08 Studies 1. Cross-validities obtained via rMonte Carlo procedure by Borman, Toquam

Reporting Differences Between Spacecraft Sequence Files

NASA Technical Reports Server (NTRS)

Khanampompan, Teerapat; Gladden, Roy E.; Fisher, Forest W.

2010-01-01

A suite of computer programs, called seq diff suite, reports differences between the products of other computer programs involved in the generation of sequences of commands for spacecraft. These products consist of files of several types: replacement sequence of events (RSOE), DSN keyword file [DKF (wherein DSN signifies Deep Space Network)], spacecraft activities sequence file (SASF), spacecraft sequence file (SSF), and station allocation file (SAF). These products can include line numbers, request identifications, and other pieces of information that are not relevant when generating command sequence products, though these fields can result in the appearance of many changes to the files, particularly when using the UNIX diff command to inspect file differences. The outputs of prior software tools for reporting differences between such products include differences in these non-relevant pieces of information. In contrast, seq diff suite removes the fields containing the irrelevant pieces of information before processing to extract differences, so that only relevant differences are reported. Thus, seq diff suite is especially useful for reporting changes between successive versions of the various products and in particular flagging difference in fields relevant to the sequence command generation and review process.

Commander islands as the significant point for monitoring some dangerous changes in the Beringia ecosystem

Treesearch

Vladimir Sevostianov

2007-01-01

This paper explores the many natural, historical, and economic reasons the Commander Islands are an essential focal point for field expedition work and finally for conservation projects in the unique ecosystem of the North Pacific. Around the Commander Islands we can find a biologically productive and diverse marine environment. For example, it is one of the richest...

Universal adaptive torque control for PM motors for field-weakening region operation

DOEpatents

Royak, Semyon [Beachwood, OH; Harbaugh, Mark M [Richfield, OH; Breitzmann, Robert J [South Russel, OH; Nondahl, Thomas A [Wauwatosa, WI; Schmidt, Peter B [Franklin, WI; Liu, Jingbo [Milwaukee, WI

2011-03-29

The invention includes a motor controller and method for controlling a permanent magnet motor. In accordance with one aspect of the present technique, a permanent magnet motor is controlled by, among other things, receiving a torque command, determining a normalized torque command by normalizing the torque command to a characteristic current of the motor, determining a normalized maximum available voltage, determining an inductance ratio of the motor, and determining a direct-axis current based upon the normalized torque command, the normalized maximum available voltage, and the inductance ratio of the motor.

A natural command language for C/3/I applications

NASA Astrophysics Data System (ADS)

Mergler, J. P.

1980-03-01

The article discusses the development of a natural command language and a control and analysis console designed to simplify the task of the operator in field of Command, Control, Communications, and Intelligence. The console is based on a DEC LSI-11 microcomputer, supported by 16-K words of memory and a serial interface component. Discussion covers the language, which utilizes English and a natural syntax, and how it is integrated with the hardware. It is concluded that results have demonstrated the effectiveness of this natural command language.

The "grep" command but not FusionMap, FusionFinder or ChimeraScan captures the CIC-DUX4 fusion gene from whole transcriptome sequencing data on a small round cell tumor with t(4;19)(q35;q13).

PubMed

Panagopoulos, Ioannis; Gorunova, Ludmila; Bjerkehagen, Bodil; Heim, Sverre

2014-01-01

Whole transcriptome sequencing was used to study a small round cell tumor in which a t(4;19)(q35;q13) was part of the complex karyotype but where the initial reverse transcriptase PCR (RT-PCR) examination did not detect a CIC-DUX4 fusion transcript previously described as the crucial gene-level outcome of this specific translocation. The RNA sequencing data were analysed using the FusionMap, FusionFinder, and ChimeraScan programs which are specifically designed to identify fusion genes. FusionMap, FusionFinder, and ChimeraScan identified 1017, 102, and 101 fusion transcripts, respectively, but CIC-DUX4 was not among them. Since the RNA sequencing data are in the fastq text-based format, we searched the files using the "grep" command-line utility. The "grep" command searches the text for specific expressions and displays, by default, the lines where matches occur. The "specific expression" was a sequence of 20 nucleotides from the coding part of the last exon 20 of CIC (Reference Sequence: NM_015125.3) chosen since all the so far reported CIC breakpoints have occurred here. Fifteen chimeric CIC-DUX4 cDNA sequences were captured and the fusion between the CIC and DUX4 genes was mapped precisely. New primer combinations were constructed based on these findings and were used together with a polymerase suitable for amplification of GC-rich DNA templates to amplify CIC-DUX4 cDNA fragments which had the same fusion point found with "grep". In conclusion, FusionMap, FusionFinder, and ChimeraScan generated a plethora of fusion transcripts but did not detect the biologically important CIC-DUX4 chimeric transcript; they are generally useful but evidently suffer from imperfect both sensitivity and specificity. The "grep" command is an excellent tool to capture chimeric transcripts from RNA sequencing data when the pathological and/or cytogenetic information strongly indicates the presence of a specific fusion gene.

Apollo 16 prime and backup crewmen during geological field trip in New Mexico

NASA Image and Video Library

1971-09-09

Dr. Lee Silver (pointing foregroung), California Institute of Technology, calls a geological feature near Taos, New Mexico, to the attention of Apollo 16 prime and backup crewmen during a geological field trip. The crewmen, from left to right, are Astronauts Charles M. Duke Jr., lunar module pilot; Fred W. Haise Jr., backup commander; Edgar D. Mitchell, backup Lunar Module pilot; and John W. Young, commander.

Torque limit of PM motors for field-weakening region operation

DOEpatents

Royak, Semyon [Beachwood, OH; Harbaugh, Mark M [Richfield, OH

2012-02-14

The invention includes a motor controller and technique for controlling a permanent magnet motor. In accordance with one aspect of the present technique, a permanent magnet motor is controlled by receiving a torque command, determining a physical torque limit based on a stator frequency, determining a theoretical torque limit based on a maximum available voltage and motor inductance ratio, and limiting the torque command to the smaller of the physical torque limit and the theoretical torque limit. Receiving the torque command may include normalizing the torque command to obtain a normalized torque command, determining the physical torque limit may include determining a normalized physical torque limit, determining a theoretical torque limit may include determining a normalized theoretical torque limit, and limiting the torque command may include limiting the normalized torque command to the smaller of the normalized physical torque limit and the normalized theoretical torque limit.

3. Southwest side of quarters R (commanding officer's quarters), looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Southwest side of quarters R (commanding officer's quarters), looking east - Naval Air Station Chase Field, Quarters R, Essex Street, .43 mile South-Southeast of intersection of Texas State Highway 202 & Independence Street, Beeville, Bee County, TX

1. Northeast side of Quarters R (commanding officer's quarters), looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Northeast side of Quarters R (commanding officer's quarters), looking west - Naval Air Station Chase Field, Quarters R, Essex Street, .43 mile South-Southeast of intersection of Texas State Highway 202 & Independence Street, Beeville, Bee County, TX

2. Southeast side of Quarters R (commanding officer's quarters), looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Southeast side of Quarters R (commanding officer's quarters), looking northwest - Naval Air Station Chase Field, Quarters R, Essex Street, .43 mile South-Southeast of intersection of Texas State Highway 202 & Independence Street, Beeville, Bee County, TX

4. Northwest side of Quarters R (commanding officer's quarters), looking ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Northwest side of Quarters R (commanding officer's quarters), looking southeast - Naval Air Station Chase Field, Quarters R, Essex Street, .43 mile South-Southeast of intersection of Texas State Highway 202 & Independence Street, Beeville, Bee County, TX

KSC-03pd1111

NASA Image and Video Library

2003-04-09

KENNEDY SPACE CENTER, FLA. -- Jeff Angermeier, assigned to lead the ground operations at the Lufkin Command Center, points out a town near the Columbia debris field. KSC workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

Combat Service Support (CSS) Enabler Functional Assessment (CEFA)

DTIC Science & Technology

1998-07-01

CDR), Combined Arms Support Command (CASCOM) with a tool to aid decision making related to mitigating E/I peacetime (programmatic) and wartime risks...not be fielded by Fiscal Year (FY) 10. Based on their estimates, any decisions , especially reductions in manpower, which rely on the existence of the E...Support (CSS) enablers/initiatives (E/I), thereby providing the Commander (CDR), Combined Arms Support Command (CASCOM) with a tool to aid decision

5. Interior of Quarters R (commanding officer's quarters), living room, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Interior of Quarters R (commanding officer's quarters), living room, looking northwest - Naval Air Station Chase Field, Quarters R, Essex Street, .43 mile South-Southeast of intersection of Texas State Highway 202 & Independence Street, Beeville, Bee County, TX

Air Force Intelligence Officer Targeteers: A Discussion on Specialization

DTIC Science & Technology

2011-06-10

significant force management problems for several reasons ( Galway et al. 2005, 48). The first reason was attributed to having no dedicated career field...manager to coordinate joint command and major command (MAJCOM) personnel requirements ( Galway et al. 2005, 51). Lack of a full time career field...intelligence work was required ( Galway et al. 2005, 48). RAND also noted that a tenuous balance existed between acquiring breadth and depth as a company

Producing Military Commanders: A Systemic Exploration of the Development Environment

DTIC Science & Technology

2010-04-15

understand the characteristics of career-long capabilities that the Army is seeking in field-grade officers. Field-grade commanders provide the...yet they have quite some trouble dealing with the sequential aspects of time. He cited a musical melody as distinguished from the individual tones...impact of decisions made at this level. Much like Dorner’s musical melody metaphor, decisions made at this level are as musical notes played from a

The Operational Commander’s Role in Planning and Executing a Successful Campaign

DTIC Science & Technology

1992-04-20

44 IS. PRICE CODE RIDGWAY IN KOREAN WAR AS CDR 8th ARMY ________ It. SECURITY CLASSIFICATION 10. SECURITY CLASSIFICATION 13. SECURITY CLASSIFICATION...Field-Marshal Slim as the 14th Army commander in Burma; General MacArthur in the World War II Cartwheel Operation and General Ridgway as the 8th Army...64 :. Introduction In his book, Command in War , Martin Van Creveld referred to the period of strategic

Military Review: Operation Desert Shield/Desert Storm

DTIC Science & Technology

1991-09-01

areas of responsibility and ob- where he could best interact with key operation- jectives to influence the outcome of the battle. al wmmanders: the...Air component command- Key to successful operational command was er for air support, the Saudi Joint Forces Coin- the interaction of the two command...opterira~~ d-~ a~ ini ection as in available news people in the field. - Vtnmam.... The operational securiy/top safety prMAlem Dese ~amnind actio oereid

Army Posture Statement: A Statement on the Posture of the United States Army, 2009

DTIC Science & Technology

2009-05-07

Research Institute Army Physical Readiness Training (FM 3-22.02) Army Preparatory School Army Prepositioned Stocks (APS) Army Reserve Employer Relations...ARFORGEN Army Force Generation AFRICOM Africa Command AMAP Army Medical Action Plan AMC Army Material Command APA Army Prepositioned Stocks AR Army...Ordnance Disposal ES2 Every Soldier a Sensor ETF Enterprise Task Force FCS Future Combat Systems FM Field Manual FORSCOM Forces Command FY Fiscal Year

Literature review on medical incident command.

PubMed

Rimstad, Rune; Braut, Geir Sverre

2015-04-01

It is not known what constitutes the optimal emergency management system, nor is there a consensus on how effectiveness and efficiency in emergency response should be measured or evaluated. Literature on the role and tasks of commanders in the prehospital emergency services in the setting of mass-casualty incidents has not been summarized and published. This comprehensive literature review addresses some of the needs for future research in emergency management through three research questions: (1) What are the basic assumptions underlying incident command systems (ICSs)? (2) What are the tasks of ambulance and medical commanders in the field? And (3) How can field commanders' performances be measured and assessed? A systematic literature search in MEDLINE, PubMed, PsycINFO, Embase, Cochrane Central Register of Controlled Trials, Cochrane Library, ISI Web of Science, Scopus, International Security & Counter Terrorism Reference Center, Current Controlled Trials, and PROSPERO covering January 1, 1990 through March 1, 2014 was conducted. Reference lists of included literature were hand searched. Included papers were analyzed using Framework synthesis. The literature search identified 6,049 unique records, of which, 76 articles and books where included in qualitative synthesis. Most ICSs are described commonly as hierarchical, bureaucratic, and based on military principles. These assumptions are contested strongly, as is the applicability of such systems. Linking of the chains of command in cooperating agencies is a basic difficulty. Incident command systems are flexible in the sense that the organization may be expanded as needed. Commanders may command by direction, by planning, or by influence. Commanders' tasks may be summarized as: conducting scene assessment, developing an action plan, distributing resources, monitoring operations, and making decisions. There is considerable variation between authors in nomenclature and what tasks are included or highlighted. There are no widely acknowledged measurement tools of commanders' performances, though several performance indicators have been suggested. The competence and experience of the commanders, upon which an efficient ICS has to rely, cannot be compensated significantly by plans and procedures, or even by guidance from superior organizational elements such as coordination centers. This study finds that neither a certain system or structure, or a specific set of plans, are better than others, nor can it conclude what system prerequisites are necessary or sufficient for efficient incident management. Commanders need to be sure about their authority, responsibility, and the functional demands posed upon them.

Prepare the Army for War. A Historical Overview of the Army Training and Doctrine Command, 1973 - 1993

DTIC Science & Technology

1993-01-01

liaison officers at the other’s equivalent major schools-armor, aviation, air defense, field artillery, engineer , infantry, signal, ordnance... Engineer Center and Fort Belvoir, the Infantry Center and Fort Benning, the Air Defense Center and Fort Bliss, the Transportation Center and Fort...administered by the commander of the Araor Center and Fort Knox. TRADOC had 16 Army branch schools. Eight schools--the Air Defense, Armor, Engineer , Field

Organization and Functions: Field Operating Agencies of The Judge Advocate General

DTIC Science & Technology

1989-03-20

Criminal Investi- gation Command. (6) Army Military Command Contract Law Specialist Training Program, which trains officers in providing legal advice and...opera- tions in the functional areas of contract law . (7) Patents, Copyrights, and Trademarks Division, which— (a) Manages the administration, control

MILES Training and Evaluation Test, USAREUR: Battalion Command Group Training

DTIC Science & Technology

1980-06-01

battle. Highly artificial limitations on the re- sources available to the commander reduce the level of stress and in- volvement of the staff and could...battle but were not simulated in the field exercise. This helped stimulate staff involve- ment in the exercise, but created artificialities in the scenario...indicators that will provide the player commander and staff the necessary intellignece to do planning and any last minute changes so they are prepared to meet

A design proposal of a certain missile tactical command system based on Beidou satellite communication and GPS positioning techniques

NASA Astrophysics Data System (ADS)

Ma, Jian; Hao, Yongsheng; Miao, Jian; Zhang, Jianmao

2007-11-01

This paper introduced a design proposal of tactical command system that applied to a kind of anti-tank missile carriers. The tactical command system was made up of embedded computer system based on PC104 bus, Linux operating system, digital military map, Beidou satellite communication equipments and GPS positioning equipments. The geographic coordinates was measured by the GPS receiver, the positioning data, commands and information were transmitted real-time between tactical command systems, tactical command systems and command center, by the Beidou satellite communication systems. The Beidou satellite communication equipments and GPS positioning equipments were integrated to an independent module, exchanging data with embedded computer through RS232 serial ports and USB ports. The decision support system software based on information fusion, calculates positioning data, geography information and battle field information synthetically, shows the position of allies and the position of enemy on the military map, and assesses the various threats of different enemy objects, educes a situation assessment and threat assessment.

STS-102 / Expedition 1 Crew Return Ceremony at Ellington Field.

NASA Image and Video Library

2001-03-22

JSC2001-E-08317 (22 March 2001) --- Members of the Expedition One crew await opportunities to individually address a crowd gathered at Ellington Field to honor their return to Houston. return. Pictured from the left are cosmonaut Vasily Tsibliev, Deputy Director of the Gagarin Cosmonaut Training Center in Star City; cosmonaut Sergei K. Krikalev, Expedition One flight engineer; astronaut William M. (Bill) Shepherd, mission commander; and Yuri P. Gidzenko, Soyuz commander; along with Joseph Rothenberg, NASA Associate Administrator for Space Flight.

Use of Semi-Autonomous Tools for ISS Commanding and Monitoring

NASA Technical Reports Server (NTRS)

Brzezinski, Amy S.

2014-01-01

As the International Space Station (ISS) has moved into a utilization phase, operations have shifted to become more ground-based with fewer mission control personnel monitoring and commanding multiple ISS systems. This shift to fewer people monitoring more systems has prompted use of semi-autonomous console tools in the ISS Mission Control Center (MCC) to help flight controllers command and monitor the ISS. These console tools perform routine operational procedures while keeping the human operator "in the loop" to monitor and intervene when off-nominal events arise. Two such tools, the Pre-positioned Load (PPL) Loader and Automatic Operators Recorder Manager (AutoORM), are used by the ISS Communications RF Onboard Networks Utilization Specialist (CRONUS) flight control position. CRONUS is responsible for simultaneously commanding and monitoring the ISS Command & Data Handling (C&DH) and Communications and Tracking (C&T) systems. PPL Loader is used to uplink small pieces of frequently changed software data tables, called PPLs, to ISS computers to support different ISS operations. In order to uplink a PPL, a data load command must be built that contains multiple user-input fields. Next, a multiple step commanding and verification procedure must be performed to enable an onboard computer for software uplink, uplink the PPL, verify the PPL has incorporated correctly, and disable the computer for software uplink. PPL Loader provides different levels of automation in both building and uplinking these commands. In its manual mode, PPL Loader automatically builds the PPL data load commands but allows the flight controller to verify and save the commands for future uplink. In its auto mode, PPL Loader automatically builds the PPL data load commands for flight controller verification, but automatically performs the PPL uplink procedure by sending commands and performing verification checks while notifying CRONUS of procedure step completion. If an off-nominal condition occurs during procedure execution, PPL Loader notifies CRONUS through popup messages, allowing CRONUS to examine the situation and choose an option of how PPL loader should proceed with the procedure. The use of PPL Loader to perform frequent, routine PPL uplinks offloads CRONUS to better monitor two ISS systems. It also reduces procedure performance time and decreases risk of command errors. AutoORM identifies ISS communication outage periods and builds commands to lock, playback, and unlock ISS Operations Recorder files. Operation Recorder files are circular buffer files of continually recorded ISS telemetry data. Sections of these files can be locked from further writing, be played back to capture telemetry data that occurred during an ISS loss of signal (LOS) period, and then be unlocked for future recording use. Downlinked Operation Recorder files are used by mission support teams for data analysis, especially if failures occur during LOS. The commands to lock, playback, and unlock Operations Recorder files are encompassed in three different operational procedures and contain multiple user-input fields. AutoORM provides different levels of automation for building and uplinking the commands to lock, playback, and unlock Operations Recorder files. In its automatic mode, AutoORM automatically detects ISS LOS periods, then generates and uplinks the commands to lock, playback, and unlock Operations Recorder files when MCC regains signal with ISS. AutoORM also features semi-autonomous and manual modes which integrate CRONUS more into the command verification and uplink process. AutoORMs ability to automatically detect ISS LOS periods and build the necessary commands to preserve, playback, and release recorded telemetry data greatly offloads CRONUS to perform more high-level cognitive tasks, such as mission planning and anomaly troubleshooting. Additionally, since Operations Recorder commands contain numerical time input fields which are tedious for a human to manually build, AutoORM's ability to automatically build commands reduces operational command errors. PPL Loader and AutoORM demonstrate principles of semi-autonomous operational tools that will benefit future space mission operations. Both tools employ different levels of automation to perform simple and routine procedures, thereby offloading human operators to perform higher-level cognitive tasks. Because both tools provide procedure execution status and highlight off-nominal indications, the flight controller is able to intervene during procedure execution if needed. Semi-autonomous tools and systems that can perform routine procedures, yet keep human operators informed of execution, will be essential in future long-duration missions where the onboard crew will be solely responsible for spacecraft monitoring and control.

The School of Hard Knocks: The Development of Close Air Support in Burma during the Second World War

DTIC Science & Technology

2015-05-23

Group CAOC Combined Air Operations Center CAS Close Air Support CBI China-Burma-India EAC Eastern Air Command FM Field Manual JP Joint...Command ( EAC ) solved problems identified by the American Volunteer Group (AVG) in 1942. EAC’s doctrine, procedures, and techniques laid the foundation for...named the Eastern Air Command ( EAC ), and oversaw the air-land cooperation during the Allied counter-offensive into Burma throughout 1943 and 1944.8 The

Commander Brand shaves in front of forward middeck lockers

NASA Technical Reports Server (NTRS)

1982-01-01

Commander Brand, wearing shorts, shaves in front of forward middeck lockers using personal hygiene mirror assembly (assy). Open modular locker single tray assy, Field Sequential (FS) crew cabin camera, communications kit assy mini headset (HDST) and HDST interface unit (HIU), personal hygiene kit, and meal tray assemblies appear in view.

64 Years Later, Korean War Vet Finally Comes Home | DoDLive

Science.gov Websites

; Bruce Erickson said. It wasn't. According to the Defense POW/MIA Accounting Agency, Gene is one of 128 /MIA Accounting Command and Central Identification Laboratory showed Clayton the documents proving his Families, Rotator and tagged Brainerd, Clayton Erickson, Department of POW/MIA Accounting Agency, DNA

Recombination Promoted by DNA Viruses: Phage λ to Herpes Simplex Virus

PubMed Central

Weller, Sandra K.; Sawitzke, James A.

2015-01-01

The purpose of this review is to explore recombination strategies in DNA viruses. Homologous recombination is a universal genetic process that plays multiple roles in the biology of all organisms, including viruses. Recombination and DNA replication are interconnected, with recombination being essential for repairing DNA damage and supporting replication of the viral genome. Recombination also creates genetic diversity, and viral recombination mechanisms have important implications for understanding viral origins as well as the dynamic nature of viral-host interactions. Both bacteriophage λ and herpes simplex virus (HSV) display high rates of recombination, both utilizing their own proteins and commandeering cellular proteins to promote recombination reactions. We focus primarily on λ and HSV, as they have proven amenable to both genetic and biochemical analysis and have recently been shown to exhibit some surprising similarities that will guide future studies. PMID:25002096

Autonomously Generating Operations Sequences for a Mars Rover Using Artificial Intelligence-Based Planning

NASA Astrophysics Data System (ADS)

Sherwood, R.; Mutz, D.; Estlin, T.; Chien, S.; Backes, P.; Norris, J.; Tran, D.; Cooper, B.; Rabideau, G.; Mishkin, A.; Maxwell, S.

2001-07-01

This article discusses a proof-of-concept prototype for ground-based automatic generation of validated rover command sequences from high-level science and engineering activities. This prototype is based on ASPEN, the Automated Scheduling and Planning Environment. This artificial intelligence (AI)-based planning and scheduling system will automatically generate a command sequence that will execute within resource constraints and satisfy flight rules. An automated planning and scheduling system encodes rover design knowledge and uses search and reasoning techniques to automatically generate low-level command sequences while respecting rover operability constraints, science and engineering preferences, environmental predictions, and also adhering to hard temporal constraints. This prototype planning system has been field-tested using the Rocky 7 rover at JPL and will be field-tested on more complex rovers to prove its effectiveness before transferring the technology to flight operations for an upcoming NASA mission. Enabling goal-driven commanding of planetary rovers greatly reduces the requirements for highly skilled rover engineering personnel. This in turn greatly reduces mission operations costs. In addition, goal-driven commanding permits a faster response to changes in rover state (e.g., faults) or science discoveries by removing the time-consuming manual sequence validation process, allowing rapid "what-if" analyses, and thus reducing overall cycle times.

Always Wanted to Hack the Pentagon? DoD Says Bring It

Science.gov Websites

test and find vulnerabilities in the department's applications, websites and networks, he added Resolve/Foal Eagle 2010, a joint U.S. and South Korean command-post exercise with computer-based command-post exercise with computer-based simulations and field exercises. Cook said other networks

KSC-03pd1112

NASA Image and Video Library

2003-04-09

KENNEDY SPACE CENTER, FLA. -- Eric Baker, a United Space Alliance project engineer at Kennedy Space Center, (right) works at the Lufkin Command Center to track hazardous tank finds. KSC workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

Crystal Field Splittings of the Hund Ground States of Nd(N) Ions in S4 Symmetry: Theory and Application to the Ga(3+) Site of Gd3Sc2Ga3O12.

DTIC Science & Technology

1984-03-01

DRDAR-TSS-S (STINFO) ATTN DRXRES-RTL, TECH LIBRARY ABERDEN PROVING GROUND , MD 21005 NATICK, MA 01762 23 %.. * ,w...DRXSY-MP (LIBRARY) ABERDEEN PROVING GROUND , MD 21005 UNDER SECRETARY OF DEFENSE RES £ ENGINEERING COMMANDER ATTN TECHNICAL LIBRARY, 3C128 US ARMY MISSILE...SANDS MISSILE RANGE, N 88002 ABERDEEN PROVING GROUND , MD 21005 DIRECTOR COMMANDER 08 RMM BALLISTIC RESEARCH LABORATORY US ARMY TROOP SUPPORT COMMAND AT

UAV field demonstration of social media enabled tactical data link

NASA Astrophysics Data System (ADS)

Olson, Christopher C.; Xu, Da; Martin, Sean R.; Castelli, Jonathan C.; Newman, Andrew J.

2015-05-01

This paper addresses the problem of enabling Command and Control (C2) and data exfiltration functions for missions using small, unmanned, airborne surveillance and reconnaissance platforms. The authors demonstrated the feasibility of using existing commercial wireless networks as the data transmission infrastructure to support Unmanned Aerial Vehicle (UAV) autonomy functions such as transmission of commands, imagery, metadata, and multi-vehicle coordination messages. The authors developed and integrated a C2 Android application for ground users with a common smart phone, a C2 and data exfiltration Android application deployed on-board the UAVs, and a web server with database to disseminate the collected data to distributed users using standard web browsers. The authors performed a mission-relevant field test and demonstration in which operators commanded a UAV from an Android device to search and loiter; and remote users viewed imagery, video, and metadata via web server to identify and track a vehicle on the ground. Social media served as the tactical data link for all command messages, images, videos, and metadata during the field demonstration. Imagery, video, and metadata were transmitted from the UAV to the web server via multiple Twitter, Flickr, Facebook, YouTube, and similar media accounts. The web server reassembled images and video with corresponding metadata for distributed users. The UAV autopilot communicated with the on-board Android device via on-board Bluetooth network.

Epstein-Barr Viral Productive Amplification Reprograms Nuclear Architecture, DNA Replication and Histone Deposition

PubMed Central

Chiu, Ya-Fang; Sugden, Arthur U.; Sugden, Bill

2014-01-01

Summary The spontaneous transition of Epstein-Barr Virus (EBV) from latency to productive infection is infrequent, making its analysis in the resulting mixed cell populations difficult. We engineered cells to support this transition efficiently and developed EBV DNA variants that could be visualized and measured as fluorescent signals over multiple cell cycles. This approach revealed that EBV’s productive replication began synchronously for viral DNAs within a cell but asynchronously between cells. EBV DNA amplification was delayed until early S-phase and occurred in factories characterized by the absence of cellular DNA and histones, by a sequential redistribution of PCNA, and by localization away from the nuclear periphery. The earliest amplified DNAs lacked histones accompanying a decline in four histone chaperones. Thus, EBV transitions from being dependent on the cellular replication machinery during latency to commandeering both that machinery and nuclear structure for its own reproductive needs. PMID:24331459

Aerodynamic Characteristics of SC1095 and SC1094 R8 Airfoils

DTIC Science & Technology

2003-12-01

Development, and Engineering Command Ames Research Center Moffett Field, California December 2003 National Aeronautics and Space Administration Ames...60A ROTOR BLADE AND AIRFOILS ................................................................................... 2 EVALUATION OF SECTION CHARACTERISTICS...Characteristics of SC1095 and SC1094 R8 Airfoils WILLIAM G. BOUSMAN Aeroflightdynamics Directorate U.S. Army Research, Development, and Engineering Command Ames

Neuronal basis of covert spatial attention in the frontal eye field.

PubMed

Thompson, Kirk G; Biscoe, Keri L; Sato, Takashi R

2005-10-12

The influential "premotor theory of attention" proposes that developing oculomotor commands mediate covert visual spatial attention. A likely source of this attentional bias is the frontal eye field (FEF), an area of the frontal cortex involved in converting visual information into saccade commands. We investigated the link between FEF activity and covert spatial attention by recording from FEF visual and saccade-related neurons in monkeys performing covert visual search tasks without eye movements. Here we show that the source of attention signals in the FEF is enhanced activity of visually responsive neurons. At the time attention is allocated to the visual search target, nonvisually responsive saccade-related movement neurons are inhibited. Therefore, in the FEF, spatial attention signals are independent of explicit saccade command signals. We propose that spatially selective activity in FEF visually responsive neurons corresponds to the mental spotlight of attention via modulation of ongoing visual processing.

Quick response airborne command post communications

NASA Astrophysics Data System (ADS)

Blaisdell, Randy L.

1988-08-01

National emergencies and strategic crises come in all forms and sizes ranging from natural disasters at one end of the scale up to and including global nuclear warfare at the other. Since the early 1960s the U.S. Government has spent billions of dollars fielding airborne command posts to ensure continuity of government and the command and control function during times of theater conventional, theater nuclear, and global nuclear warfare. Unfortunately, cost has prevented the extension of the airborne command post technology developed for these relatively unlikely events to the lower level, though much more likely to occur, crises such as natural disasters, terrorist acts, political insurgencies, etc. This thesis proposes the implementation of an economical airborne command post concept to address the wide variety of crises ignored by existing military airborne command posts. The system is known as the Quick Response Airborne Command Post (QRAC Post) and is based on the exclusive use of commercially owned and operated aircraft, and commercially available automated data processing and communications resources. The thesis addresses the QRAC Post concept at a systems level and is primarily intended to demonstrate how current technology can be exploited to economically achieve a national objective.

DC-magnetic field vector measurement

NASA Technical Reports Server (NTRS)

Schmidt, R.

1981-01-01

A magnetometer experiment was designed to determine the local magnetic field by measuring the total of the Earth's magnetic field and that of an unknown spacecraft. The measured field vector components are available to all onboard experiments via the Spacelab command and data management system. The experiment consists of two parts, an electronic box and the magnetic field sensor. The sensor includes three independent measuring flux-gate magnetometers, each measuring one component. The physical background is the nonlinearity of the B-H curve of a ferrite material. Two coils wound around a ferrite rod are necessary. One of them, a tank coil, pumps the ferrite rod at approximately 20 kilohertz. As a consequence of the nonlinearity, many harmonics can be produced. The second coil (i.e., the detection coil) resonates to the first harmonic. If an unknown dc or low-frequency magnetic field exists, the amplitude of the first harmonic is a measure for the unknown magnetic field. The voltages detected by the sensors are to be digitized and transferred to the command and data management system.

Performance Test for the SIGMA Communication System

NASA Astrophysics Data System (ADS)

Jeong, Seonyeong; Lee, Hyojeong; Lee, Seongwhan; Shin, Jehyuck; Lee, Jungkyu; Jin, Ho

2016-12-01

Scientific CubeSat with Instruments for Global Magnetic Fields and Radiations (SIGMA) is a 3-U size CubeSat that will be operated in low earth orbit (LEO). The SIGMA communication system uses a very high frequency (VHF) band for uplink and an ultra high frequency (UHF) band for downlink. Both frequencies belong to an amateur band. The ground station that communicates with SIGMA is located at Kyung Hee Astronomical Observatory (KHAO). For reliable communication, we carried out a laboratory (LAB) test and far-field tests between the CubeSat and a ground station. In the field test, we considered test parameters such as attenuation, antenna deployment, CubeSat body attitude, and Doppler frequency shift in transmitting commands and receiving data. In this paper, we present a communication performance test of SIGMA, a link budget analysis, and a field test process. We also compare the link budget with the field test results of transmitting commands and receiving data.

Training Effectiveness and Cost Iterative Technique (TECIT). Volume 2. Cost Effectiveness Analysis

DTIC Science & Technology

1988-07-01

Moving Tank in a Field Exercise A The task cluster identified as tank commander’s station/tank gunnery and the sub-task of firing an M250 grenade launcher...Firing Procedures, Task Number 171-126-1028. I OBJECTIVE: Given an Ml tank with crew, loaded M250 I grenade launcher, the commander’s station powered up

Theory of Near-Field Scanning with a Probe Array

DTIC Science & Technology

2014-01-01

AIR FORCE RESEARCH LABORATORY SENSORS DIRECTORATE WRIGHT-PATTERSON AIR FORCE BASE, OH 45433-7320 AIR FORCE MATERIEL COMMAND...AFRL/RYMH) Sensors Directorate, Air Force Research Laboratory Wright-Patterson Air Force Base, OH 45433-7320 Air Force Materiel Command, United...S) AND ADDRESS(ES) 10. SPONSORING/MONITORING AGENCY ACRONYM(S) Air Force Research Laboratory Sensors Directorate Wright-Patterson Air Force Base

9. Photocopy of command flow chart of NIKE Battalion, Headquarters ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Photocopy of command flow chart of NIKE Battalion, Headquarters Battery and Missile Battery from Procedures and Drills for NIKE Ajax System, Department of the Army Field Manual, FM-44-80 from Institute for Military History, Carlisle Barracks, Carlisle, PA 1956 - NIKE Missile Battery PR-79, East Windsor Road south of State Route 101, Foster, Providence County, RI

Web 2.0 Systems in the Brigade Combat Team as an Enabler of Mission Command: A Dialectic in Information Discourse

DTIC Science & Technology

2016-06-10

WEB 2.0 SYSTEMS IN THE BRIGADE COMBAT TEAM AS AN ENABLER OF MISSION COMMAND: A DIALECTIC IN INFORMATION DISCOURSE A thesis......This qualitative research in the field of information science aims to examine the use of Web 2.0 systems in the Brigade Combat Team as an enabler of

Photocopy of recent aerial photograph (from U.S. Army Support Command ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of recent aerial photograph (from U.S. Army Support Command Hawaii, Wheeler Army Air Base, Hawaii) Photographer unknown, Circa 1990 AERIAL VIEW SHOWING MAIN SECTION OF BASE, BETWEEN KUNIA ROAD, WILIKINA DRIVE, AND McMAHON ROAD, AS WELL AS ADJACENT PINEAPPLE FIELDS, AND LAKE WILSON. - Schofield Barracks Military Reservation, Wilikina Drive & Kunia Road, Wahiawa, Honolulu County, HI

Navigator Command Potential: An Analysis of U.S. Air Force Pilot Attitudes toward the Job Satisfaction Characteristics of U.S. Air Force Navigators

DTIC Science & Technology

1990-09-01

than many of the other officer career fields. In 1986, Marchewka reported that job dissatisfaction among navigators was *probably because their jobs are...Company, Inc., 1935. 30. Marchewka , Maj Peter S. Job Attitudes of USAF Pilots and Navigators. Unpublished report No. 86-1610. Air Command and Staff

Command Post Program Study. Volume 1 - Main Report

DTIC Science & Technology

1973-03-01

Field Manual 30-9, Military Intelligence Battalion - Field Am. Washington: March 1968. _ Field Manual 32-20, Electronic Warfare. Washington: 14...Mechanized). A-18 TOE 30-88T, Military Intelligence Support Detachment, Military Intelligence Battalion, Field Army. TOE 30-206T, Headquarters and... Field Manual 30-9, Military Intelligence Battalion - Field Army. Washington: March 1968. _ Field Manual 32-20, Electronic Warfare. Washington: 14

ARC-2008-ACD08-0200-075

NASA Image and Video Library

2008-09-11

September 11th remembrance ceremony held in front of NASA Research Park Bldg-17 (Lunar Science Institute) hosted by the American Legion, Post 881, Moffett Field. Welcome by Moffett Field Post Commander Carolann Wunderlin.

ARC-2008-ACD08-0200-003

NASA Image and Video Library

2008-09-11

September 11th remembrance ceremony held in front of NASA Research Park Bldg-17 (Lunar Science Institute) hosted by the American Legion, Post 881, Moffett Field. Welcome by Moffett Field Post Commander Carolann Wunderlin.

MARTA: a suite of Java-based tools for assigning taxonomic status to DNA sequences.

PubMed

Horton, Matthew; Bodenhausen, Natacha; Bergelson, Joy

2010-02-15

We have created a suite of Java-based software to better provide taxonomic assignments to DNA sequences. We anticipate that the program will be useful for protistologists, virologists, mycologists and other microbial ecologists. The program relies on NCBI utilities including the BLAST software and Taxonomy database and is easily manipulated at the command-line to specify a BLAST candidate's query-coverage or percent identity requirements; other options include the ability to set minimal consensus requirements (%) for each of the eight major taxonomic ranks (Domain, Kingdom, Phylum, ...) and whether to consider lower scoring candidates when the top-hit lacks taxonomic classification.

Autonomous Commanding of the WIRE Spacecraft

NASA Technical Reports Server (NTRS)

Prior, Mike; Walyus, Keith; Saylor, Rick

1999-01-01

This paper presents the end-to-end design architecture for an autonomous commanding capability to be used on the Wide Field Infrared Explorer (WIRE) mission for the uplink of command loads during unattended station contacts. The WIRE mission is the fifth and final mission of NASA's Goddard Space Flight Center Small Explorer (SMEX) series to be launched in March of 1999. Its primary mission is the targeting of deep space fields using an ultra-cooled infrared telescope. Due to its mission design WIRE command loads are large (approximately 40 Kbytes per 24 hours) and must be performed daily. To reduce the cost of mission operations support that would be required in order to uplink command loads, the WIRE Flight Operations Team has implemented an autonomous command loading capability. This capability allows completely unattended operations over a typical two- day weekend period. The key factors driving design and implementation of this capability were: 1) Integration with already existing ground system autonomous capabilities and systems, 2) The desire to evolve autonomous operations capabilities based upon previous SMEX operations experience 3) Integration with ground station operations - both autonomous and man-tended, 4) Low cost and quick implementation, and 5) End-to-end system robustness. A trade-off study was performed to examine these factors in light of the low-cost, higher-risk SMEX mission philosophy. The study concluded that a STOL (Spacecraft Test and Operations Language) based script, highly integrated with other scripts used to perform autonomous operations, was best suited given the budget and goals of the mission. Each of these factors is discussed to provide an overview of the autonomous operations capabilities implemented for the mission. The capabilities implemented on the WIRE mission are an example of a low-cost, robust, and efficient method for autonomous command loading when implemented with other autonomous features of the ground system. They can be used as a design and implementation template by other small satellite missions interested in evolving toward autonomous and lower cost operations.

BpWrapper: BioPerl-based sequence and tree utilities for rapid prototyping of bioinformatics pipelines.

PubMed

Hernández, Yözen; Bernstein, Rocky; Pagan, Pedro; Vargas, Levy; McCaig, William; Ramrattan, Girish; Akther, Saymon; Larracuente, Amanda; Di, Lia; Vieira, Filipe G; Qiu, Wei-Gang

2018-03-02

Automated bioinformatics workflows are more robust, easier to maintain, and results more reproducible when built with command-line utilities than with custom-coded scripts. Command-line utilities further benefit by relieving bioinformatics developers to learn the use of, or to interact directly with, biological software libraries. There is however a lack of command-line utilities that leverage popular Open Source biological software toolkits such as BioPerl ( http://bioperl.org ) to make many of the well-designed, robust, and routinely used biological classes available for a wider base of end users. Designed as standard utilities for UNIX-family operating systems, BpWrapper makes functionality of some of the most popular BioPerl modules readily accessible on the command line to novice as well as to experienced bioinformatics practitioners. The initial release of BpWrapper includes four utilities with concise command-line user interfaces, bioseq, bioaln, biotree, and biopop, specialized for manipulation of molecular sequences, sequence alignments, phylogenetic trees, and DNA polymorphisms, respectively. Over a hundred methods are currently available as command-line options and new methods are easily incorporated. Performance of BpWrapper utilities lags that of precompiled utilities while equivalent to that of other utilities based on BioPerl. BpWrapper has been tested on BioPerl Release 1.6, Perl versions 5.10.1 to 5.25.10, and operating systems including Apple macOS, Microsoft Windows, and GNU/Linux. Release code is available from the Comprehensive Perl Archive Network (CPAN) at https://metacpan.org/pod/Bio::BPWrapper . Source code is available on GitHub at https://github.com/bioperl/p5-bpwrapper . BpWrapper improves on existing sequence utilities by following the design principles of Unix text utilities such including a concise user interface, extensive command-line options, and standard input/output for serialized operations. Further, dozens of novel methods for manipulation of sequences, alignments, and phylogenetic trees, unavailable in existing utilities (e.g., EMBOSS, Newick Utilities, and FAST), are provided. Bioinformaticians should find BpWrapper useful for rapid prototyping of workflows on the command-line without creating custom scripts for comparative genomics and other bioinformatics applications.

Computers for Command and Control: An Airland Battle Requirement!

DTIC Science & Technology

1984-05-01

systems can enhance communications, improve data management, and support decision making through information display (SEE REVERSE) JAN 173 E~lNOS~SIISLT...organizations to improve communications, enhance data management, and support decision making through graphical display techniques and mathematical...tactical commander’s control of maneuver forces. There are many reasons for the Army’s apparent inability to develop and field these systems. Among the

USACDEC Experimentation Manual

DTIC Science & Technology

1981-10-01

Commander, Instrumentation Command (Prov) who is responsible for the cinematic form of the films. The writing requirements for discrete sections of the...level of simulated realism required. Higher levels of simulated realism will require higher degrees of control to insure the test events occur as...experimentation, the "enemy" created to add realism . Aggressor forces may be represented by live troops In the field or by mechanical targets with or

Effects of Debris Entrainment and Multi-Phase Flow on Plug Loading in an MX Trench.

DTIC Science & Technology

1978-09-15

gas stream of density (pg) and velocity (Vg) is: -., * -) - * 2~ TD FD Pg (V P V) Vp-Vg I CD( TD ) (A.1) 4 where the drag coefficient (CD) is defined by...ATTN: FCPR ATTN: Code L53 , J. Forrest Field Command Naval Facilities Engineering Command Defense Nuclear Agency ATTN: Code 09M22C Livermore Division

OpenPET Hardware, Firmware, Software, and Board Design Files

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abu-Nimeh, Faisal; Choong, Woon-Sengq; Moses, William W.

OpenPET is an open source, flexible, high-performance, and modular data acquisition system for a variety of applications. The OpenPET electronics are capable of reading analog voltage or current signals from a wide variety of sensors. The electronics boards make extensive use of field programmable gate arrays (FPGAs) to provide flexibility and scalability. Firmware and software for the FPGAs and computer are used to control and acquire data from the system. The command and control flow is similar to the data flow, however, the commands are initiated from the computer similar to a tree topology (i.e., from top-to-bottom). Each node inmore » the tree discovers its parent and children, and all addresses are configured accordingly. A user (or a script) initiates a command from the computer. This command will be translated and encoded to the corresponding child (e.g., SB, MB, DB, etc.). Consecutively, each node will pass the command to its corresponding child(ren) by looking at the destination address. Finally, once the command reaches its desired destination(s) the corresponding node(s) execute(s) the command and send(s) a reply, if required. All the firmware, software, and the electronics board design files are distributed through the OpenPET website (http://openpet.lbl.gov).« less

KSC-03pd1110

NASA Image and Video Library

2003-04-09

KENNEDY SPACE CENTER, FLA. -- NASA Kennedy Space Center engineer Lamar Russell, who is serving as team lead for debris siting reports outside of Texas, points out to his Lufkin Command Center team a location targeted for a grid search. Kennedy Space Center workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

A Magneto-Inductive Sensor Based Wireless Tongue-Computer Interface

PubMed Central

Huo, Xueliang; Wang, Jia; Ghovanloo, Maysam

2015-01-01

We have developed a noninvasive, unobtrusive magnetic wireless tongue-computer interface, called “Tongue Drive,” to provide people with severe disabilities with flexible and effective computer access and environment control. A small permanent magnet secured on the tongue by implantation, piercing, or tissue adhesives, is utilized as a tracer to track the tongue movements. The magnetic field variations inside and around the mouth due to the tongue movements are detected by a pair of three-axial linear magneto-inductive sensor modules mounted bilaterally on a headset near the user’s cheeks. After being wirelessly transmitted to a portable computer, the sensor output signals are processed by a differential field cancellation algorithm to eliminate the external magnetic field interference, and translated into user control commands, which could then be used to access a desktop computer, maneuver a powered wheelchair, or control other devices in the user’s environment. The system has been successfully tested on six able-bodied subjects for computer access by defining six individual commands to resemble mouse functions. Results show that the Tongue Drive system response time for 87% correctly completed commands is 0.8 s, which yields to an information transfer rate of ~130 b/min. PMID:18990653

Astronaut Charles Duke works at front of Lunar Roving Vehicle

NASA Image and Video Library

1972-04-23

AS16-116-18607 (23 April 1972) --- Astronaut Charles M. Duke Jr. works at the front of the Lunar Roving Vehicle (LRV) parked in this rock field at a North Ray Crater geological site during the mission's third extravehicular activity (EVA) on April 23, 1972. Astronaut John W. Young took this picture with a 70mm Hasselblad camera. While astronauts Young, commander; and Duke, lunar module pilot; descended in the Apollo 16 Lunar Module (LM) "Orion" to explore the Descartes highlands landing site on the moon, astronaut Thomas K. Mattingly II, command module pilot, remained with the Command and Service Modules (CSM) "Casper" in lunar orbit.

STS-104 Crew Return, Ellington Field, Building 990

NASA Image and Video Library

2001-07-26

JSC2001-E-22790 (25 July 2001) --- Astronauts Janet L. Kavandi (left), STS-104 mission specialist, and Steven W. Lindsey, mission commander, are greeted by JSC Acting Director Roy S. Estess following crew arrival at Ellington Field.

Evaluation of aero Commander propeller acoustic data: Static operations

NASA Technical Reports Server (NTRS)

Piersol, A. G.; Wilby, E. G.; Wilby, J. F.

1978-01-01

Acoustic data are analyzed from a series of ground tests performed on an Aero Commander propeller-driven aircraft with an array of microphones flush-mounted on one side of the fuselage. The analyses were concerned with the propeller blade passage noise during static operation at several different engine speeds and included calculations of the magnitude and phase of the blade passage tones, the amplitude stability of the tones, and the spatial phase and coherence of the tones. The results indicate that the pressure field impinging on the fuselage represents primarily aerodynamic (near field) effects in the plane of the propeller at all frequencies. Forward and aft of the propeller plane aerodynamic effects still dominate the pressure field at frequencies below 200 Hz; but at higher frequencies, the pressure field is due to acoustic propagation from an equivalent center located about 0.15 to 0.30 blade diameters inboard from the propeller hub.

Field trauma care in the 21st century.

PubMed

O'Reilly, D; König, T; Tai, N

2008-12-01

The nature of trauma care on the modern battlefield is changing quickly. Leading figures in UK field trauma care spoke at a recent meeting of the Haywood Club. The challenge of modern warfare, the evolving evacuation chain and the command and governance of field trauma care were explored.

User's guide to HYPOINVERSE-2000, a Fortran program to solve for earthquake locations and magnitudes

USGS Publications Warehouse

Klein, Fred W.

2002-01-01

Hypoinverse is a computer program that processes files of seismic station data for an earthquake (like p wave arrival times and seismogram amplitudes and durations) into earthquake locations and magnitudes. It is one of a long line of similar USGS programs including HYPOLAYR (Eaton, 1969), HYPO71 (Lee and Lahr, 1972), and HYPOELLIPSE (Lahr, 1980). If you are new to Hypoinverse, you may want to start by glancing at the section “SOME SIMPLE COMMAND SEQUENCES” to get a feel of some simpler sessions. This document is essentially an advanced user’s guide, and reading it sequentially will probably plow the reader into more detail than he/she needs. Every user must have a crust model, station list and phase data input files, and glancing at these sections is a good place to begin. The program has many options because it has grown over the years to meet the needs of one the largest seismic networks in the world, but small networks with just a few stations do use the program and can ignore most of the options and commands. History and availability. Hypoinverse was originally written for the Eclipse minicomputer in 1978 (Klein, 1978). A revised version for VAX and Pro-350 computers (Klein, 1985) was later expanded to include multiple crustal models and other capabilities (Klein, 1989). This current report documents the expanded Y2000 version and it supercedes the earlier documents. It serves as a detailed user's guide to the current version running on unix and VAX-alpha computers, and to the version supplied with the Earthworm earthquake digitizing system. Fortran-77 source code (Sun and VAX compatible) and copies of this documentation is available via anonymous ftp from computers in Menlo Park. At present, the computer is swave.wr.usgs.gov and the directory is /ftp/pub/outgoing/klein/hyp2000. If you are running Hypoinverse on one of the Menlo Park EHZ or NCSN unix computers, the executable currently is ~klein/hyp2000/hyp2000. New features. The Y2000 version of Hypoinverse includes all of the previous capabilities, but adds Y2000 formats to those defined earlier. In most cases, the new formats add 2 digits to the year field to accommodate the century. Other fields are sometimes rearranged or expanded to accommodate a better field order. The Y2000 formats are invoked with the “200” command. When the Y2000 flag is turned on, all files are read and written in the new format and there is no mixing of format types in a single run. Some formats without a date field, like station files, have not changed. A separate program called 2000CONV has been written to convert old formats to new. Other new features, like expanded station names, calculating amplitude magnitudes from a variety of digital seismometers, station history files, interactive earthquake processing, and locations from CUSP (Caltech USGS Seismic Processing) binary files have been added. General features. Hypoinverse will locate any number of events in an input file, which can be in one of several different formats. Any or all of printout, summary or archive output may be produced. Hypoinverse is driven by user commands. The various commands define input and output files, set adjustable parameters, and solve for locations of a file of earthquake data using the parameters and files currently set. It is both interactive and "batch" in that commands may be executed either from the keyboard or from a file. You execute the commands in a file by typing @filename at the Hypoinverse prompt. Users may either supply parameters on the command line, or omit them and are prompted interactively. The current parameter values are displayed and may be taken as defaults by pressing just the RETURN key after the prompt. This makes the program very easy to use, providing you can remember the names of the commands. Combining commands with and without their required parameters into a command file permits a variety of customized procedures such as automatic input of crustal model and station data, but prompting for a different phase file each time. All commands are 3 letters long and most require one or more parameters or file names. If they appear on a line with a command, character strings such as filenames must be enclosed in apostrophes (single quotes). Appendix 1 gives this and other free-format rules for supplying parameters, which are parsed in Fortran. When several parameters are required following a command, any of them may be omitted by replacing them with null fields (see appendix 1). A null field leaves that parameter unchanged from its current or default value. When you start HYPOINVERSE, default values are in effect for all parameters except file names. Hypoinverse is a complicated program with many features and options. Many of these "advanced" or seldom used features are documented here, but are more detailed than a typical user needs to read about when first starting with the program. I have put some of this material in smaller type so that a first time user can concentrate on the more important information.

The Influence of Future Command, Control, Communications, and Computers (C4) on Doctrine and the Operational Commander's Decision-Making Process

NASA Technical Reports Server (NTRS)

Mayer, Michael G.

1996-01-01

Future C4 systems will alter the traditional balance between force and information, having a profound influence on doctrine and the operational commander's decision making process. The Joint Staff's future vision of C4 is conceptualized in 'C4I for the Warrior' which envisions a joint C4I architecture providing timely sensor to shoot information direct to the warfighter. C4 system must manage and filter an overwhelming amount of information; deal with interoperability issues; overcome technological limitations; meet emerging security requirements; and protect against 'Information Warfare.' Severe budget constraints necessitate unified control of C4 systems under singular leadership for the common good of all the services. In addition, acquisition policy and procedures must be revamped to allow new technologies to be fielded quickly; and the commercial marketplace will become the preferred starting point for modernization. Flatter command structures are recommended in this environment where information is available instantaneously. New responsibilities for decision making at lower levels are created. Commanders will have to strike a balance between exerting greater control and allowing subordinates enough flexibility to maintain initiative. Clearly, the commander's intent remains the most important tool in striking this balance.

OPSATCOM Field Measurements. Volume 1

DTIC Science & Technology

1976-06-01

ADVANCED RESEARCH PROJECTS AGENCY AF SPACE & MISSILE SYSTEMS ORGANIZATION -. TECHNICAL ý IBRARY (3) SKX (DR. D. BARRY) (3) OFFICE OF THE IRECTOR OF DEFENSE...FORCE CAMBRIDGE kL.LSEARCH CENTER DEFENs r FELFC’OMMUNICATI[NS& COMMAND & tECHNICAL LIBRAHY r’ONTROL SYSTEMS NATIONAL AERONAUTICS AND SPACE...STREET T NAVAL. ELECTRONIC SYSTEMS COMMAND SANTA MONICA, CA 90496 NELEX.031031 IT. B. HUGHES) (3) UNIVERSITY OF CALIFORNIA, SAN DIEGO NEL X 3102 (M

Ionospheric Irregularity Physics Modelling.

DTIC Science & Technology

1982-02-09

NUMBERS Washington, DC 20375 62715H; 47-0889-0-2 II CONTROLLING OFFICE NAME AND ADDRESS 12 REPORT DATE Defense Nuclear Agency February 9, 1982 Washington... CONTROL TECHNICAL CENTER COMMANDER PE TAGON RM SF 685 FIELD COMMAND WASHINGTON, D.C. 20301 DEFENSE NUCLEAR AGENCY OICY ATTN C-650 KIRTLAND, AFR, NM...BUILDINICG KIRTLAND AFB, NM 87115 1400 WILSON BLVD. OICY ATTN DOCUMENT CONTROL ARLINGTON, 11A. 22209 OICY ATTN NUCLEAR MONITORING RESEARCH JOINT CHIEFS OF

STS-102 / Expedition 1 Crew Return Ceremony at Ellington Field.

NASA Image and Video Library

2001-03-22

JSC2001-E-08325 (22 March 2001) --- Some of the participants of the Expedition One and STS-102 crew return ceremony applaud one of the speakers. Pictured from the left are cosmonaut Vasily Tsibliev, Deputy Director of the Gagarin Cosmonaut Training Center in Star City; cosmonaut Sergei K. Krikalev, Expedition One flight engineer; astronaut William M. (Bill) Shepherd, mission commander; and Yuri P. Gidzenko, Soyuz commander.

USSR Report, Military Affairs.

DTIC Science & Technology

1986-09-02

Monitoring the Fulfillment of Pledges"] [Text] Northern Group of Forces—That night the tank company commanded by Captain I. Kuznetsov was to rehearse a...are commanders who reason something like this: We’ll go into the field, we can learn everything there. In particular, Capt Kuznetsov , it emerged...this. 36 But in the regiment where Capt Kuznetsov serves there are subunits which now know to achieve high results in combat training while using

Command, Control, and the Common Defense

DTIC Science & Technology

1996-10-01

as in two books, Our Air Force: The Keystone of National Defense and Winged Defense: The Development and Possibilities of Modern Air Power, Economic...Command first took the field against the Germans during the North African campaign in 1943, control of tactical air operations was fragmented at both...remained intensely loyal to their original strategic ideas. 42 The tactical success of which Brodie wrote was gained at some cost in the North African

Army Aviation and the Mission Command Warfighting Function

DTIC Science & Technology

2017-06-09

MAJ Wyatt Anthony Britten, 82 pages. This qualitative research in the field of the Mission Command Warfighting Function with a focus on Army...ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) 9-06-2017 2. REPORT TYPE Master’s Thesis 3. DATES COVERED (From - To) AUG 2016 – JUNE 2017 4. TITLE...DISTRIBUTION / AVAILABILITY STATEMENT Approved for Public Release; Distribution is Unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT This qualitative

The Virtual Combat Air Staff. The Promise of Information Technologies,

DTIC Science & Technology

1996-08-07

development of specialists . The need for advance planning and orchestration of a growing variety of sup- port functions has required the commander to... ability for almost unlim- ited access to command authorities and fielded forces via worldwide communications, increased situational awareness, and the...mercially developed systems that incorporate the latest technologies, training programs will have to adapt to the rapid pace at which such technologies are

Field experiments using SPEAR: a speech control system for UGVs

NASA Astrophysics Data System (ADS)

Chhatpar, Siddharth R.; Blanco, Chris; Czerniak, Jeffrey; Hoffman, Orin; Juneja, Amit; Pruthi, Tarun; Liu, Dongqing; Karlsen, Robert; Brown, Jonathan

2009-05-01

This paper reports on a Field Experiment carried out by the Human Research and Engineering Directorate at Ft. Benning to evaluate the efficacy of using speech to control an Unmanned Ground Vehicle (UGV) concurrently with a handcontroller. The SPEAR system, developed by Think-A-Move, provides speech-control of UGVs. The system picks up user-speech in the ear canal with an in-ear microphone. This property allows it to work efficiently in high-noise environments, where traditional speech systems, employing external microphones, fail. It has been integrated with an iRobot PackBot 510 with EOD kit. The integrated system allows the hand-controller to be supplemented with speech for concurrent control. At Ft. Benning, the integrated system was tested by soldiers from the Officer Candidate School. The Experiment had dual focus: 1) Quantitative measurement of the time taken to complete each station and the cognitive load on users; 2) Qualitative evaluation of ease-of-use and ergonomics through soldier-feedback. Also of significant benefit to Think-A-Move was soldier-feedback on the speech-command vocabulary employed: What spoken commands are intuitive, and how the commands should be executed, e.g., limited-motion vs. unlimited-motion commands. Overall results from the Experiment are reported in the paper.

The impact of time and field conditions on brown bear (Ursus arctos) faecal DNA amplification

USGS Publications Warehouse

Murphy, M.A.; Kendall, K.C.; Robinson, A.; Waits, L.P.

2007-01-01

To establish longevity of faecal DNA samples under varying summer field conditions, we collected 53 faeces from captive brown bears (Ursus arctos) on a restricted vegetation diet. Each faeces was divided, and one half was placed on a warm, dry field site while the other half was placed on a cool, wet field site on Moscow Mountain, Idaho, USA. Temperature, relative humidity, and dew point data were collected on each site, and faeces were sampled for DNA extraction at <1, 3, 6, 14, 30, 45, and 60 days. Faecal DNA sample viability was assessed by attempting PCR amplification of a mitochondrial DNA (mtDNA) locus (???150 bp) and a nuclear DNA (nDNA) microsatellite locus (180-200 bp). Time in the field, temperature, and dew point impacted mtDNA and nDNA amplification success with the greatest drop in success rates occurring between 1 and 3 days. In addition, genotyping errors significantly increased over time at both field sites. Based on these results, we recommend collecting samples at frequent transect intervals and focusing sampling efforts during drier portions of the year when possible. ?? 2007 Springer Science+Business Media, Inc.

Astronaut Neil Armstrong - Rock Sample Study - Geological Field Trip - TX

NASA Image and Video Library

1969-03-03

S69-25198 (25 Feb. 1969) --- Astronaut Neil A. Armstrong, commander of the Apollo 11 prime crew, studies rock sample during a geological field trip to the Quitman Mountains area near the Fort Quitman ruins in far west Texas.

Electrophoretic and field-effect graphene for all-electrical DNA array technology.

PubMed

Xu, Guangyu; Abbott, Jeffrey; Qin, Ling; Yeung, Kitty Y M; Song, Yi; Yoon, Hosang; Kong, Jing; Ham, Donhee

2014-09-05

Field-effect transistor biomolecular sensors based on low-dimensional nanomaterials boast sensitivity, label-free operation and chip-scale construction. Chemical vapour deposition graphene is especially well suited for multiplexed electronic DNA array applications, since its large two-dimensional morphology readily lends itself to top-down fabrication of transistor arrays. Nonetheless, graphene field-effect transistor DNA sensors have been studied mainly at single-device level. Here we create, from chemical vapour deposition graphene, field-effect transistor arrays with two features representing steps towards multiplexed DNA arrays. First, a robust array yield--seven out of eight transistors--is achieved with a 100-fM sensitivity, on par with optical DNA microarrays and at least 10 times higher than prior chemical vapour deposition graphene transistor DNA sensors. Second, each graphene acts as an electrophoretic electrode for site-specific probe DNA immobilization, and performs subsequent site-specific detection of target DNA as a field-effect transistor. The use of graphene as both electrode and transistor suggests a path towards all-electrical multiplexed graphene DNA arrays.

Visual Basic VPython Interface: Charged Particle in a Magnetic Field

NASA Astrophysics Data System (ADS)

Prayaga, Chandra

2006-12-01

A simple Visual Basic (VB) to VPython interface is described and illustrated with the example of a charged particle in a magnetic field. This interface allows data to be passed to Python through a text file read by Python. The first component of the interface is a user-friendly data entry screen designed in VB, in which the user can input values of the charge, mass, initial position and initial velocity of the particle, and the magnetic field. Next, a command button is coded to write these values to a text file. Another command button starts the VPython program, which reads the data from the text file, numerically solves the equation of motion, and provides the 3d graphics animation. Students can use the interface to run the program several times with different data and observe changes in the motion.

The ingestible thermal monitoring system

NASA Technical Reports Server (NTRS)

Cutchis, Protagoras N.; Hogrefe, Arthur F.; Lesho, Jeffery C.

1988-01-01

A thermal monitoring system for measuring body core temperatures was developed that contains an ingestible pill which is both commandable and rechargeable, and which uses magnetic induction for command and telemetry as well as for recharging. The pill electronics consist of a battery power source, a crystal-controlled oscillator that drives a small air coil, and a command detection circuit. The resulting 262-kHz magnetilc field can be easily detected from a distance of 1 m. The pill oscillator functions at voltages less than 1 V, supplied by a single Ni-Cd battery, which must be recharged after 72 h of continuous transmission. The pill can be recalibrated periodically to compensate for long-term drift.

Lunar Roving Vehicle parked in lunar depression on slope of Stone Mountain

NASA Image and Video Library

1972-04-22

AS16-107-17473 (22 April 1972) --- The Lunar Roving Vehicle (LRV) appears to be parked in a deep lunar depression, on the slope of Stone Mountain. This photograph of the lunar scene at Station No. 4 was taken during the second Apollo 16 extravehicular activity (EVA) at the Descartes landing site. A sample collection bag is in the right foreground. Note field of small boulders at upper right. While astronauts John W. Young, commander, and Charles M. Duke Jr., lunar module pilot, descended in the Lunar Module (LM) "Orion" to explore the moon, astronaut Thomas K. Mattingly II, command module pilot, remained with the Command and Service Modules (CSM) in lunar orbit.

Annual Report to the President and the Congress

DTIC Science & Technology

2004-01-01

planning process has determined is needed to meet the military tasks as- signed to the Department by the President in his national security strategy...Commands" document, which formally as- signs forces to combatant commanders. The Global Force Management Process subsumes two developmen- tal measures...www.t2net.org), which was signed by Deputy Secretary of Defense Paul Wolfowitz on June 10, 2003, is a road map to developing and fielding dynamic

Army Networks: Opportunities Exist to Better Utilize Results from Network Integration Evaluations

DTIC Science & Technology

2013-08-01

monitor operations; a touch screen-based mission command planning tool; and an antenna mast . The Army will field only one of these systems in capability...Office JTRS Joint Tactical Radio System NIE Network Integration Evaluation OSD Office of the Secretary of Defense SUE System under Evaluation...command systems . A robust transport layer capable of delivering voice, data, imagery, and video to the tactical edge (i.e., the forward battle lines

Astronauts Young and Duke study rock formations on simulated lunar traverse

NASA Technical Reports Server (NTRS)

1971-01-01

Astronauts John W. Young, right, prime crew commander for Apollo 16, and Charles M. Duke Jr., lunar module pilot, study rock formations along their simulated lunar traverse route. The prime and backup commanders and lunar module pilots for Apollo 16 took part in the two-day geology field trip and simulations in the Coso Range, near Ridgecrest, California. The training was conducted at the U.S. Naval Ordnance Test Station.

PR-PR: Cross-Platform Laboratory Automation System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Linshiz, G; Stawski, N; Goyal, G

To enable protocol standardization, sharing, and efficient implementation across laboratory automation platforms, we have further developed the PR-PR open-source high-level biology-friendly robot programming language as a cross-platform laboratory automation system. Beyond liquid-handling robotics, PR-PR now supports microfluidic and microscopy platforms, as well as protocol translation into human languages, such as English. While the same set of basic PR-PR commands and features are available for each supported platform, the underlying optimization and translation modules vary from platform to platform. Here, we describe these further developments to PR-PR, and demonstrate the experimental implementation and validation of PR-PR protocols for combinatorial modified Goldenmore » Gate DNA assembly across liquid-handling robotic, microfluidic, and manual platforms. To further test PR-PR cross-platform performance, we then implement and assess PR-PR protocols for Kunkel DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic and manual platforms.« less

PR-PR: cross-platform laboratory automation system.

PubMed

Linshiz, Gregory; Stawski, Nina; Goyal, Garima; Bi, Changhao; Poust, Sean; Sharma, Monica; Mutalik, Vivek; Keasling, Jay D; Hillson, Nathan J

2014-08-15

To enable protocol standardization, sharing, and efficient implementation across laboratory automation platforms, we have further developed the PR-PR open-source high-level biology-friendly robot programming language as a cross-platform laboratory automation system. Beyond liquid-handling robotics, PR-PR now supports microfluidic and microscopy platforms, as well as protocol translation into human languages, such as English. While the same set of basic PR-PR commands and features are available for each supported platform, the underlying optimization and translation modules vary from platform to platform. Here, we describe these further developments to PR-PR, and demonstrate the experimental implementation and validation of PR-PR protocols for combinatorial modified Golden Gate DNA assembly across liquid-handling robotic, microfluidic, and manual platforms. To further test PR-PR cross-platform performance, we then implement and assess PR-PR protocols for Kunkel DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic and manual platforms.

An improved DNA force field for ssDNA interactions with gold nanoparticles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiang, Xiankai; Huai, Ping; Fan, Chunhai

The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones “protecting” hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thusmore » the “protection” by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.« less

An improved DNA force field for ssDNA interactions with gold nanoparticles

NASA Astrophysics Data System (ADS)

Jiang, Xiankai; Gao, Jun; Huynh, Tien; Huai, Ping; Fan, Chunhai; Zhou, Ruhong; Song, Bo

2014-06-01

The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones "protecting" hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thus the "protection" by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.

An improved DNA force field for ssDNA interactions with gold nanoparticles.

PubMed

Jiang, Xiankai; Gao, Jun; Huynh, Tien; Huai, Ping; Fan, Chunhai; Zhou, Ruhong; Song, Bo

2014-06-21

The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones "protecting" hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thus the "protection" by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.

Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples

USGS Publications Warehouse

Pilliod, David S.; Goldberg, Caren S.; Arkle, Robert S.; Waits, Lisette P.

2013-01-01

Environmental DNA (eDNA) methods for detecting aquatic species are advancing rapidly, but with little evaluation of field protocols or precision of resulting estimates. We compared sampling results from traditional field methods with eDNA methods for two amphibians in 13 streams in central Idaho, USA. We also evaluated three water collection protocols and the influence of sampling location, time of day, and distance from animals on eDNA concentration in the water. We found no difference in detection or amount of eDNA among water collection protocols. eDNA methods had slightly higher detection rates than traditional field methods, particularly when species occurred at low densities. eDNA concentration was positively related to field-measured density, biomass, and proportion of transects occupied. Precision of eDNA-based abundance estimates increased with the amount of eDNA in the water and the number of replicate subsamples collected. eDNA concentration did not vary significantly with sample location in the stream, time of day, or distance downstream from animals. Our results further advance the implementation of eDNA methods for monitoring aquatic vertebrates in stream habitats.

Balancing the Interactions of Ions, Water, and DNA in the Drude Polarizable Force Field

PubMed Central

2015-01-01

Recently we presented a first-generation all-atom Drude polarizable force field for DNA based on the classical Drude oscillator model, focusing on optimization of key dihedral angles followed by extensive validation of the force field parameters. Presently, we describe the procedure for balancing the electrostatic interactions between ions, water, and DNA as required for development of the Drude force field for DNA. The proper balance of these interactions is shown to impact DNA stability and subtler conformational properties, including the conformational equilibrium between the BI and BII states, and the A and B forms of DNA. The parametrization efforts were simultaneously guided by gas-phase quantum mechanics (QM) data on small model compounds and condensed-phase experimental data on the hydration and osmotic properties of biologically relevant ions and their solutions, as well as theoretical predictions for ionic distribution around DNA oligomer. In addition, fine-tuning of the internal base parameters was performed to obtain the final DNA model. Notably, the Drude model is shown to more accurately reproduce counterion condensation theory predictions of DNA charge neutralization by the condensed ions as compared to the CHARMM36 additive DNA force field, indicating an improved physical description of the forces dictating the ionic solvation of DNA due to the explicit treatment of electronic polarizability. In combination with the polarizable DNA force field, the availability of Drude polarizable parameters for proteins, lipids, and carbohydrates will allow for simulation studies of heterogeneous biological systems. PMID:24874104

Beyond the Gap: A Historical Perspective on World War II River Crossings

DTIC Science & Technology

2013-05-23

crossing’s defense, and provide sufficient space to organize the vehicles as they cross from the friendly side. The bridgehead commander has many tasks...combat units are across and back into the fight, the senior commander still have work to do in the space behind the crossing sites, called the rear...Procedure,” Gilbert R. Cook Papers , Box 9, Dwight D. Eisenhower Presidential Library, Abilene, Kansas, 110. The document stated that field manuals and

2005 9th Annual Army Small Business Conference

DTIC Science & Technology

2005-11-03

field commanders who conduct acquisitions. All the Army’s major commands located in the United States will be represented. The conference...Engineer Squad Vehicle i r i l Mobile Gun System il yst Medical Evacuation Vehicle i l v ti i l Reconnaissance Vehicle iss i l Mortar Carrier rt r rri r...Manned Systems Unmanned Air Vehicles Class I ARV-A (L) Small (Manpackable) UGV Non-Line of Sight Cannon Non-Line of Sight Mortar Medical Treatment and

The Concurrent Implementation of Radio Frequency Identification and Unique Item Identification at Naval Surface Warfare Center, Crane, IN as a Model for a Navy Supply Chain Application

DTIC Science & Technology

2007-12-01

electromagnetic theory related to RFID in his works “ Field measurements using active scatterers” and “Theory of loaded scatterers”. At the same time...Business Case Analysis BRE: Bangor Radio Frequency Evaluation C4ISR: Command, Control, Communications, Computers, Intelligence, Surveillance...Surveillance EEDSKs: Early Entry Deployment Support Kits EHF: Extremely High Frequency xvi EUCOM: European Command FCC : Federal Communications

A Case Study Analysis of a System Acquisition by a Field Activity

DTIC Science & Technology

1980-06-01

following personnel assisted in the preparation of this procurement plan: Mr. T. L. M. Wiegant Ms. J. Weaver Commander D. M. Chism Lieutenant Commander M...AUTHORITY (SSA). Rear Admiral V.T. EDSALL, SC, USN 2.2 PROCURING CONTRACTING OFFICER (PCO). Cofmander David M. CHISM , SC, USN (Regional Contracting...California, 1977. 5. Brozen, Dr. Yale, "Commentary," Barron’s National Business and Financial Weekly, 25 January 1971, p. 1. 6. Chism , CDR David, NISTARS

Radiation Evaluation of the AM2901A Microprocessor.

DTIC Science & Technology

1980-08-01

Fitz , Jr. ATTN: DRDAR-LCA-PD 4 cy ATTN: TITL U.S. Army Communications R&D Command Field Command ATTN: D. Huewe Defense Nuclear Agency ATTN: FCPR U.S...Stahl ATTN: Technical Library ATTN: T. Flanagan Mission Research Corp, Santa Barbara Johns Hopkins University, Laurel ATTN: C. Longmire Applied...Bernardino Sperry Rand Corp, Phoenix ATTN: F. Fay ATTN: D. Schow ATTN: M. Gorman ATTN: P. Kitter Sperry Univac, St. Paul ATTN: J. Inda TRW Systems and

Whole Exome Analysis of Early Onset Alzheimer’s Disease

DTIC Science & Technology

2017-04-01

manufacturer’s protocol at the HIHG Center for Genome Technology. After capture, the DNA was tested for uniform enrichment of targets via quantitative PCR...CONTRACTING ORGANIZATION: University of Miami Miami, FL 33136 REPORT DATE: April 2017 TYPE OF REPORT: Annual PREPARED FOR: U.S. Army Medical Research ...U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 10. SPONSOR/MONITOR’S ACRONYM(S) 11. SPONSOR/MONITOR’S REPORT NUMBER

A Two-Center Validation of "Patient Does Not Follow Commands" and Three Other Simplified Measures to Replace the Glasgow Coma Scale for Field Trauma Triage.

PubMed

Hopkins, Emily; Green, Steven M; Kiemeney, Michael; Haukoos, Jason S

2018-05-02

Out-of-hospital personnel worldwide calculate the 13-point Glasgow Coma Scale (GCS) score as a routine part of field trauma triage. We wish to independently validate a simpler binary assessment to replace the GCS for this task. We analyzed trauma center registries from Loma Linda University Health (2003 to 2015) and Denver Health Medical Center (2009 to 2015) to compare the binary assessment "patient does not follow commands" (ie, GCS motor score <6) with GCS score less than or equal to 13 for the prediction of 5 trauma outcomes: emergency intubation, clinically significant brain injury, need for neurosurgical intervention, Injury Severity Score greater than 15, and mortality. As a secondary analysis, we similarly evaluated 3 other measures simpler than the GCS: GCS motor score less than 5, Simplified Motor Score, and the "alert, voice, pain, unresponsive" scale. In this analysis of 47,973 trauma patients, we found that the binary assessment "patient does not follow commands" was essentially identical to GCS score less than or equal to 13 for the prediction of all 5 trauma outcomes, with slightly superior positive likelihood ratios (eg, those for mortality 2.37 versus 2.13) offsetting slightly inferior negative ones (eg, those for mortality 0.25 versus 0.24) and its graphic depiction of sensitivity versus specificity superimposing the GCS prediction curve. We found similar results for the 3 other simplified measures. In this 2-center external validation, we confirmed that a simple binary assessment-"patient does not follow commands"-could effectively replace the more complicated GCS for field trauma triage. Copyright © 2018 American College of Emergency Physicians. Published by Elsevier Inc. All rights reserved.

Apollo 12 crewmembers during geological field trip

NASA Image and Video Library

1969-10-24

S69-55662 (10 Oct. 1969) --- Astronauts Alan L. Bean (left) and Charles Conrad Jr., the two crewmen of the Apollo 12 lunar landing mission who are scheduled to participate in two lengthy periods of extravehicular activity (EVA) on the lunar surface, are pictured during a geological field trip and training at a simulated lunar surface area near Flagstaff, Arizona. Here Conrad, the Apollo 12 commander, gets a close look through hand lens at the stratigraphy (study of strata or layers beneath the surface) of a man-dug hole, while Bean, the Apollo 12 mission's lunar module pilot, looks on. The topography in this area, with several man-made modifications, resembles very closely much of the topography found on the lunar surface. While Conrad and Bean explore the lunar surface (plans call for Apollo 12 spacecraft to land in the Sea of Storms), astronaut Richard F. Gordon Jr., command module pilot for the Apollo 12 mission, will remain with the Command and Service Modules (CSM) in lunar orbit. The Apollo 12 mission is scheduled to lift off from Cape Kennedy on Nov. 14, 1969.

NASA Electronic Library System (NELS) database schema, version 1.2

NASA Technical Reports Server (NTRS)

Melebeck, Clovis J.

1991-01-01

The database tables used by NELS version 1.2 are discussed. To provide the current functional capability offered by NELS, nineteen tables were created with ORACLE. Each table lists the ORACLE table name and provides a brief description of the tables intended use or function. The following sections cover four basic categories of tables: NELS object classes, NELS collections, NELS objects, and NELS supplemental tables. Also included in each section is a definition and/or relationship of each field to other fields or tables. The primary key(s) for each table is indicated with a single asterisk (*), while foreign keys are indicated with double asterisks (**). The primary key(s) indicate the key(s) which uniquely identifies a record for that table. The foreign key(s) is used to identify additional information in other table(s) for that record. The two appendices are the command which is used to construct the ORACLE tables for NELS. Appendix A contains the commands which create the tables which are defined in the following sections. Appendix B contains the commands which build the indices for these tables.

Cliffbot Maestro

NASA Technical Reports Server (NTRS)

Norris, Jeffrey S.; Powell, Mark W.; Fox, Jason M.; Crockett, Thomas M.; Joswig, Joseph C.

2009-01-01

Cliffbot Maestro permits teleoperation of remote rovers for field testing in extreme environments. The application user interface provides two sets of tools for operations: stereo image browsing and command generation.

Separation of large DNA molecules by applying pulsed electric field to size exclusion chromatography-based microchip

NASA Astrophysics Data System (ADS)

Azuma, Naoki; Itoh, Shintaro; Fukuzawa, Kenji; Zhang, Hedong

2018-02-01

Through electrophoresis driven by a pulsed electric field, we succeeded in separating large DNA molecules with an electrophoretic microchip based on size exclusion chromatography (SEC), which was proposed in our previous study. The conditions of the pulsed electric field required to achieve the separation were determined by numerical analyses using our originally proposed separation model. From the numerical results, we succeeded in separating large DNA molecules (λ DNA and T4 DNA) within 1600 s, which was approximately half of that achieved under a direct electric field in our previous study. Our SEC-based electrophoresis microchip will be one of the effective tools to meet the growing demand of faster and more convenient separation of large DNA molecules, especially in the field of epidemiological research of infectious diseases.

History of the Fifteenth United States Army. 21 August 1944 to 11 July 1945

DTIC Science & Technology

1946-12-13

depots together with complete inventories . c. Complete rosters by unit, grade and organization of all members of his command. d. A roster of all...and wea’pons will b@ neatly stacked by front line troops so that rapid inventory is possible. Inventory will be made by the German Fortress Command...in centrally located warehouses or field dumps at battalion level, inventoried and located on overlays, copies of which will be delivered as directed

Dust Cloud Models: Sensitivity of Calculated Transmittances to Variations in Input Parameters

DTIC Science & Technology

1981-06-01

Artillery School ATTN: DELEW-M-STO (Dr. Steven Kovel) ATTN: ATSF- CF -R (CPT James M. Watson) White Sands Missile Range, NM 88002 Fort Sill, OK 73503 Office...STEWS-PT-AL (Laurel B. Saunders) ATTN: ATSF- CF -R White Sands Missile Range, RM 88002 Fort Sill, OK 73503 Commander Commandant US Army RAD Coordinator...Army Field Artillery School ATTN: OELEW-M-STO (Dr. Steven Kovel) ATTN: ATSF- CF -R (CPT James M. Watson) White Sands Missile Range, NM 88002 Fort Sill, OK

A Prototype Decision Support System For The Selection of Ammunition Transfer Points Based on Field Artillery Role Assignments.

DTIC Science & Technology

1982-11-01

maneuver commander. In the defense, the opposite is true. CThe criteria is the centralization of fire support, since the highest level commander must...the ASP in the division rear. With this capability, all CSR levels are capable of being obtained from the ATP. 6) Travel times, both day and night, are...breakthrough below brigade level . I Procedure The changing situation of the modern battlefield does not permit calculation and input of excessive amounts

Air Land Sea Bulletin, Issue No. 2013-2, Joint Close Air Support

DTIC Science & Technology

2013-05-01

game plan between his aircraft or element and the coor- dinated attack aircraft or element (s) for a specific JTAC/FAC(A) designated target. However...5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME...Service Department of Defense field agency sponsored by the US Army Train - ing and Doctrine Command (TRADOC), Marine Corps Combat Development Command

Air Intelligence and the Search for the Center of Gravity

DTIC Science & Technology

1988-04-01

co inAIR INTELLIGENCE AND THE 0) ~ SEARCH FOR THE CENTER OF GRAVITY F LT COL CHARLES N. CULBERTSON 1988 - .- ,------.--.- Non VL AIR UNIVERSITY RLlo...During the 1930’s the future air commanders in the Air Corps’ primary doctrinal think tank, The Air Corps Tactical School, at Maxwell Field, took this...conversation with Speer after the war General Ira Eaker, the former commander of the 6th Air Force (8 AF was the AAF’s primary strategic striking arm in

The Directed Megaphone: A Theater Commander’s Means to Communicate His Vision and Intent

DTIC Science & Technology

1993-05-01

commander must have oratory skills--both logos and pathos , as described by Aristotle . He must have a flair for the dramatic to embellish his message...went on to say that persuasion depends on three elements: logos -- The truth and logical validity of what is being argued. ethos -- The speakers...presents the message ( pathos ), and who the speaker is (ethoas). Many experts in the field of communications identify ethos as the most important persuasive

Rapid Radiochemical Methods for Selected Radionuclides

EPA Pesticide Factsheets

The rapid methods documents are supplement guidance in a planned series designed to present radioanalytical laboratory personnel, Incident Commanders (and their designees), and other field response personnel.

Using Spare Logic Resources To Create Dynamic Test Points

NASA Technical Reports Server (NTRS)

Katz, Richard; Kleyner, Igor

2011-01-01

A technique has been devised to enable creation of a dynamic set of test points in an embedded digital electronic system. As a result, electronics contained in an application specific circuit [e.g., gate array, field programmable gate array (FPGA)] can be internally probed, even when contained in a closed housing during all phases of test. In the present technique, the test points are not fixed and limited to a small number; the number of test points can vastly exceed the number of buffers or pins, resulting in a compact footprint. Test points are selected by means of spare logic resources within the ASIC(s) and/or FPGA(s). A register is programmed with a command, which is used to select the signals that are sent off-chip and out of the housing for monitoring by test engineers and external test equipment. The register can be commanded by any suitable means: for example, it could be commanded through a command port that would normally be used in the operation of the system. In the original application of the technique, commanding of the register is performed via a MIL-STD-1553B communication subsystem.

Improving Control of Two Motor Controllers

NASA Technical Reports Server (NTRS)

Toland, Ronald W.

2004-01-01

A computer program controls motors that drive translation stages in a metrology system that consists of a pair of two-axis cathetometers. This program is specific to Compumotor Gemini (or equivalent) motors and the Compumotor 6K-series (or equivalent) motor controller. Relative to the software supplied with the controller, this program affords more capabilities and is easier to use. Written as a Virtual Instrument in the LabVIEW software system, the program presents an imitation control panel that the user can manipulate by use of a keyboard and mouse. There are three modes of operation: command, movement, and joystick. In command mode, single commands are sent to the controller for troubleshooting. In movement mode, distance, speed, and/or acceleration commands are sent to the controller. Position readouts from the motors and from position encoders on the translation stages are displayed in marked fields. At any time, the position readouts can be recorded in a file named by the user. In joystick mode, the program yields control of the motors to a joystick. The program sends commands to, and receives data from, the controller via a serial cable connection, using the serial-communication portion of the software supplied with the controller.

A learning controller for nonrepetitive robotic operation

NASA Technical Reports Server (NTRS)

Miller, W. T., III

1987-01-01

A practical learning control system is described which is applicable to complex robotic and telerobotic systems involving multiple feedback sensors and multiple command variables. In the controller, the learning algorithm is used to learn to reproduce the nonlinear relationship between the sensor outputs and the system command variables over particular regions of the system state space, rather than learning the actuator commands required to perform a specific task. The learned information is used to predict the command signals required to produce desired changes in the sensor outputs. The desired sensor output changes may result from automatic trajectory planning or may be derived from interactive input from a human operator. The learning controller requires no a priori knowledge of the relationships between the sensor outputs and the command variables. The algorithm is well suited for real time implementation, requiring only fixed point addition and logical operations. The results of learning experiments using a General Electric P-5 manipulator interfaced to a VAX-11/730 computer are presented. These experiments involved interactive operator control, via joysticks, of the position and orientation of an object in the field of view of a video camera mounted on the end of the robot arm.

Military Infectious Diseases Update on Vaccine Development

DTIC Science & Technology

2011-01-24

Research Program (MIDRP) Insect Vector ControlDiagnostics Prevention Treatment Infectious diseases adversely impact military operations. Vaccines...appropriate treatment and aids commanders in the field. Most militarily relevant infectious diseases are transmitted by biting insects and other...based Insect Repellent (1946) Vaccines Protectants Antiparasitic Drugs Research Effort Advanced Development Fielded Products Malaria Rapid

Apollo 12 - Bean - Conrad - during geological field trip

NASA Image and Video Library

1969-10-24

S69-55667 (10 Oct. 1969) --- Astronauts Charles Conrad Jr. and Alan L. Bean train for their upcoming Apollo 12 lunar landing mission. Here they are entering a simulated lunar surface area near Flagstaff, Arizona. Both are wearing lunar surface cameras strapped to their bodies. Conrad (left), the Apollo 12 mission commander, is carrying some of the tools from the geological tool container. The geological tool container, being carried here by Bean, the lunar module pilot, is similar to the one which will be used during scheduled extravehicular activity (EVA) periods on Nov. 19 and 20, 1969, on the lunar surface. While astronauts Conrad and Bean conduct their scheduled EVA on the moon's surface, astronaut Richard F. Gordon Jr., command module pilot, will man the Command and Service Modules (CSM) in lunar orbit.

Supramolecular gel electrophoresis of large DNA fragments.

PubMed

Tazawa, Shohei; Kobayashi, Kazuhiro; Oyoshi, Takanori; Yamanaka, Masamichi

2017-10-01

Pulsed-field gel electrophoresis is a frequent technique used to separate exceptionally large DNA fragments. In a typical continuous field electrophoresis, it is challenging to separate DNA fragments larger than 20 kbp because they migrate at a comparable rate. To overcome this challenge, it is necessary to develop a novel matrix for the electrophoresis. Here, we describe the electrophoresis of large DNA fragments up to 166 kbp using a supramolecular gel matrix and a typical continuous field electrophoresis system. C 3 -symmetric tris-urea self-assembled into a supramolecular hydrogel in tris-boric acid-EDTA buffer, a typical buffer for DNA electrophoresis, and the supramolecular hydrogel was used as a matrix for electrophoresis to separate large DNA fragments. Three types of DNA marker, the λ-Hind III digest (2 to 23 kbp), Lambda DNA-Mono Cut Mix (10 to 49 kbp), and Marker 7 GT (10 to 165 kbp), were analyzed in this study. Large DNA fragments of greater than 100 kbp showed distinct mobility using a typical continuous field electrophoresis system. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

STS-103 crew return at building 990, Ellington Field

NASA Image and Video Library

1999-12-29

Photographic documentation showing STS-103 crew return at bldg. 990, Ellington Field. Views include: Mission Specialist (MS) John M. Grunsfeld at podium (16048); MS Jean-Francois Clervoy at podium (16049); Grunsfeld signs autographs (16050); woman and child (16051); MS Claude Nicollier meets his Swiss-American fan club (16052); Clervoy holds child (16053); mission commander Curtis L. Brown signs autographs (16054, 16057); MS E. Michael Foale signs autographs (16055); MS and Payload Commander (PLC) Steven L. Smith kneels and holds child (16056); overall view of stage showing Brown at podium with crew seated behind him; from left to right: Nicollier, pilot Scott J. Kelly, Clervoy, Grunsfeld, Mr. George Abbey (JSC director), Foale and Smith (16058); Nicollier at podium (16059); Mr. George Abbey at the podium (16060): Foale ath the podium (16061); Kelly signs autographs (16062).

Evaluation of DNA Force Fields in Implicit Solvation

PubMed Central

Gaillard, Thomas; Case, David A.

2011-01-01

DNA structural deformations and dynamics are crucial to its interactions in the cell. Theoretical simulations are essential tools to explore the structure, dynamics, and thermodynamics of biomolecules in a systematic way. Molecular mechanics force fields for DNA have benefited from constant improvements during the last decades. Several studies have evaluated and compared available force fields when the solvent is modeled by explicit molecules. On the other hand, few systematic studies have assessed the quality of duplex DNA models when implicit solvation is employed. The interest of an implicit modeling of the solvent consists in the important gain in the simulation performance and conformational sampling speed. In this study, respective influences of the force field and the implicit solvation model choice on DNA simulation quality are evaluated. To this end, extensive implicit solvent duplex DNA simulations are performed, attempting to reach both conformational and sequence diversity convergence. Structural parameters are extracted from simulations and statistically compared to available experimental and explicit solvation simulation data. Our results quantitatively expose the respective strengths and weaknesses of the different DNA force fields and implicit solvation models studied. This work can lead to the suggestion of improvements to current DNA theoretical models. PMID:22043178

Developing and Fielding Information Dominance

DTIC Science & Technology

2002-01-01

Developing and Fielding Information Dominance Space and Naval Warfare Systems Command’s IT-21 Blocks 1 and 2 2002 Command and Control Research and...00-00-2002 4. TITLE AND SUBTITLE Developing and Fielding Information Dominance 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER...force levels were uncertain, the necessary role of information dominance to maintaining strategic superiority was not. Platform Centric Warfare, with its

Quantification of Plasmodiophora brassicae Using a DNA-Based Soil Test Facilitates Sustainable Oilseed Rape Production

PubMed Central

Wallenhammar, Ann-Charlotte; Gunnarson, Albin; Hansson, Fredrik; Jonsson, Anders

2016-01-01

Outbreaks of clubroot disease caused by the soil-borne obligate parasite Plasmodiophora brassicae are common in oilseed rape (OSR) in Sweden. A DNA-based soil testing service that identifies fields where P. brassicae poses a significant risk of clubroot infection is now commercially available. It was applied here in field surveys to monitor the prevalence of P. brassicae DNA in field soils intended for winter OSR production and winter OSR field experiments. In 2013 in Scania, prior to planting, P. brassicae DNA was detected in 60% of 45 fields on 10 of 18 farms. In 2014, P. brassicae DNA was detected in 44% of 59 fields in 14 of 36 farms, in the main winter OSR producing region in southern Sweden. P. brassicae was present indicative of a risk for >10% yield loss with susceptible cultivars (>1300 DNA copies g soil−1) in 47% and 44% of fields in 2013 and 2014 respectively. Furthermore, P. brassicae DNA was indicative of sites at risk of complete crop failure if susceptible cultivars were grown (>50 000 copies g−1 soil) in 14% and 8% of fields in 2013 and 2014, respectively. A survey of all fields at Lanna research station in western Sweden showed that P. brassicae was spread throughout the farm, as only three of the fields (20%) showed infection levels below the detection limit for P.brassicae DNA, while the level was >50,000 DNA copies g−1 soil in 20% of the fields. Soil-borne spread is of critical importance and soil scraped off footwear showed levels of up to 682 million spores g−1 soil. Soil testing is an important tool for determining the presence of P. brassicae and providing an indication of potential yield loss, e.g., in advisory work on planning for a sustainable OSR crop rotation. This soil test is gaining acceptance as a tool that increases the likelihood of success in precision agriculture and in applied research conducted in commercial oilseed fields and at research stations. The present application highlights the importance of prevention of disease spread by cleaning of farm equipment, footwear, etc. PMID:27135241

Quantification of Plasmodiophora brassicae Using a DNA-Based Soil Test Facilitates Sustainable Oilseed Rape Production.

PubMed

Wallenhammar, Ann-Charlotte; Gunnarson, Albin; Hansson, Fredrik; Jonsson, Anders

2016-04-22

Outbreaks of clubroot disease caused by the soil-borne obligate parasite Plasmodiophora brassicae are common in oilseed rape (OSR) in Sweden. A DNA-based soil testing service that identifies fields where P. brassicae poses a significant risk of clubroot infection is now commercially available. It was applied here in field surveys to monitor the prevalence of P. brassicae DNA in field soils intended for winter OSR production and winter OSR field experiments. In 2013 in Scania, prior to planting, P. brassicae DNA was detected in 60% of 45 fields on 10 of 18 farms. In 2014, P. brassicae DNA was detected in 44% of 59 fields in 14 of 36 farms, in the main winter OSR producing region in southern Sweden. P. brassicae was present indicative of a risk for >10% yield loss with susceptible cultivars (>1300 DNA copies g soil(-1)) in 47% and 44% of fields in 2013 and 2014 respectively. Furthermore, P. brassicae DNA was indicative of sites at risk of complete crop failure if susceptible cultivars were grown (>50 000 copies g(-1) soil) in 14% and 8% of fields in 2013 and 2014, respectively. A survey of all fields at Lanna research station in western Sweden showed that P. brassicae was spread throughout the farm, as only three of the fields (20%) showed infection levels below the detection limit for P.brassicae DNA, while the level was >50,000 DNA copies g(-1) soil in 20% of the fields. Soil-borne spread is of critical importance and soil scraped off footwear showed levels of up to 682 million spores g(-1) soil. Soil testing is an important tool for determining the presence of P. brassicae and providing an indication of potential yield loss, e.g., in advisory work on planning for a sustainable OSR crop rotation. This soil test is gaining acceptance as a tool that increases the likelihood of success in precision agriculture and in applied research conducted in commercial oilseed fields and at research stations. The present application highlights the importance of prevention of disease spread by cleaning of farm equipment, footwear, etc.

Electrical characterization of gold-DNA-gold structures in presence of an external magnetic field by means of I-V curve analysis.

PubMed

Khatir, Nadia Mahmoudi; Banihashemian, Seyedeh Maryam; Periasamy, Vengadesh; Ritikos, Richard; Abd Majid, Wan Haliza; Abdul Rahman, Saadah

2012-01-01

This work presents an experimental study of gold-DNA-gold structures in the presence and absence of external magnetic fields with strengths less than 1,200.00 mT. The DNA strands, extracted by standard method were used to fabricate a Metal-DNA-Metal (MDM) structure. Its electric behavior when subjected to a magnetic field was studied through its current-voltage (I-V) curve. Acquisition of the I-V curve demonstrated that DNA as a semiconductor exhibits diode behavior in the MDM structure. The current versus magnetic field strength followed a decreasing trend because of a diminished mobility in the presence of a low magnetic field. This made clear that an externally imposed magnetic field would boost resistance of the MDM structure up to 1,000.00 mT and for higher magnetic field strengths we can observe an increase in potential barrier in MDM junction. The magnetic sensitivity indicates the promise of using MDM structures as potential magnetic sensors.

Electrical Characterization of Gold-DNA-Gold Structures in Presence of an External Magnetic Field by Means of I–V Curve Analysis

PubMed Central

Khatir, Nadia Mahmoudi; Banihashemian, Seyedeh Maryam; Periasamy, Vengadesh; Ritikos, Richard; Majid, Wan Haliza Abd; Rahman, Saadah Abdul

2012-01-01

This work presents an experimental study of gold-DNA-gold structures in the presence and absence of external magnetic fields with strengths less than 1,200.00 mT. The DNA strands, extracted by standard method were used to fabricate a Metal-DNA-Metal (MDM) structure. Its electric behavior when subjected to a magnetic field was studied through its current-voltage (I–V) curve. Acquisition of the I–V curve demonstrated that DNA as a semiconductor exhibits diode behavior in the MDM structure. The current versus magnetic field strength followed a decreasing trend because of a diminished mobility in the presence of a low magnetic field. This made clear that an externally imposed magnetic field would boost resistance of the MDM structure up to 1,000.00 mT and for higher magnetic field strengths we can observe an increase in potential barrier in MDM junction. The magnetic sensitivity indicates the promise of using MDM structures as potential magnetic sensors. PMID:22737025

A New Methodology for Modeling National Command Level Decisionmaking in War Games and Simulations.

DTIC Science & Technology

1986-07-01

Conclusions about Utility and Development Options, The Rand Corporation, R-2945-DNA, March 1983. Drucker , Peter F., Management : Tasks, Responsibilities...looks to the worst case can readily find himself paralyzed. Of course, it is also true ’The effort to affect an opponent’s image of oneself is part of...how to manage forces on a continuing basis. So long as the broad features of the NCL-specified plan continue to appear valid, it is the military com

In Vivo Testing of Chemopreventive Agents Using the Dog Model of Spontaneous Prostate Carcinogenesis

DTIC Science & Technology

2005-03-01

2005 TYPE OF REPORT: Final PR1 PARED F-OR: U.S. Ariny Medical Rsearch nd Materiel Command Fort Detrick, Maryland 21702-5012 DISTRIBUTION STATEMENT...should not be consirued as an oliciail Department of the Army position, policy or decision unless so designated by other documentation. 1 Form Approved...trace mineral selenium and DNA damage, apoptosis, and androgens in the prostate. Our work has generated the first evidence of a non -linear, U-shaped

The Collins Center Update. Volume 1, Issue 3, December 1999

DTIC Science & Technology

1999-12-01

CDN), devel oped and executed the FORO DE ESTRATEGIA NACIONAL 2005 Hon­ du ras en el Siglo XXI (FEN 2005) {Na tional Strategy Forum 2005 Hon du­ ras...tools and processes used to make strate gic leaders. Im­ pressed with this program, Gover nor Pat ton requested a return visit with his en tire...wide command post and field train ing exer cise which tests and vali­ dates nuclear command and control, and exe cu tion proce dures. It is based on a

The new Mobile Command Center at KSC is important addition to emergency preparedness

NASA Technical Reports Server (NTRS)

2000-01-01

This camper-equipped truck known as '''Old Blue''' served as mobile field command center for the Emergency Preparedness team at KSC. It has been replaced with a larger vehicle that includes a conference room, computer work stations, mobile telephones and a fax machine, plus its own onboard generator. Besides being ready to respond in case of emergencies during launches, the vehicle must be ready to help address fires, security threats, chemical spills, terrorist attaches, weather damage or other critical situations that might face KSC or Cape Canaveral Air Force Station.

Direct observation of iron-induced conformational changes of mitochondrial DNA by high-resolution field-emission in-lens scanning electron microscopy.

PubMed Central

Yaffee, M; Walter, P; Richter, C; Müller, M

1996-01-01

When respiring rat liver mitochondria are incubated in the presence of Fe(III) gluconate, their DNA (mtDNA) relaxes from the supercoiled to the open circular form dependent on the iron dose. Anaerobiosis or antioxidants fail to completely inhibit the unwinding. High-resolution field-emission in-lens scanning electron microscopy imaging, in concert with backscattered electron detection, pinpoints nanometer-range iron colloids bound to mtDNA isolated from iron-exposed mitochondria. High-resolution field-emission in-lens scanning electron microscopy with backscattered electron detection imaging permits simultaneous detailed visual analysis of DNA topology, iron dose-dependent mtDNA unwinding, and assessment of iron colloid formation on mtDNA strands. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:8643576

Hands-free device control using sound picked up in the ear canal

NASA Astrophysics Data System (ADS)

Chhatpar, Siddharth R.; Ngia, Lester; Vlach, Chris; Lin, Dong; Birkhimer, Craig; Juneja, Amit; Pruthi, Tarun; Hoffman, Orin; Lewis, Tristan

2008-04-01

Hands-free control of unmanned ground vehicles is essential for soldiers, bomb disposal squads, and first responders. Having their hands free for other equipment and tasks allows them to be safer and more mobile. Currently, the most successful hands-free control devices are speech-command based. However, these devices use external microphones, and in field environments, e.g., war zones and fire sites, their performance suffers because of loud ambient noise: typically above 90dBA. This paper describes the development of technology using the ear as an output source that can provide excellent command recognition accuracy even in noisy environments. Instead of picking up speech radiating from the mouth, this technology detects speech transmitted internally through the ear canal. Discreet tongue movements also create air pressure changes within the ear canal, and can be used for stealth control. A patented earpiece was developed with a microphone pointed into the ear canal that captures these signals generated by tongue movements and speech. The signals are transmitted from the earpiece to an Ultra-Mobile Personal Computer (UMPC) through a wired connection. The UMPC processes the signals and utilizes them for device control. The processing can include command recognition, ambient noise cancellation, acoustic echo cancellation, and speech equalization. Successful control of an iRobot PackBot has been demonstrated with both speech (13 discrete commands) and tongue (5 discrete commands) signals. In preliminary tests, command recognition accuracy was 95% with speech control and 85% with tongue control.

KSC-2009-3103

NASA Image and Video Library

2009-05-11

CAPE CANAVERAL, Fla. – The mini-convoy is lined up on the Shuttle Landing Facility runway at NASA's Kennedy Space Center in Florida awaiting space shuttle Atlantis' launch on the STS-125 mission to service NASA's Hubble Space Telescope. The convoy is prepared to act should the shuttle need to return to the launch site in the event of an emergency. At left is the Convoy Command Vehicle which is the command post for the convoy commander. Atlantis launched successfully on time at 2:01 p.m. EDT. Atlantis' 11-day flight will include five spacewalks to refurbish and upgrade the telescope with state-of-the-art science instruments that will expand Hubble's capabilities and extend its operational lifespan through at least 2014. The payload includes a Wide Field Camera 3, Fine Guidance Sensor and the Cosmic Origins Spectrograph. Photo credit: NASA/Jack Pfaller

Modification of the coil-stretch transition by confinement

NASA Astrophysics Data System (ADS)

Doyle, Patick; Tang, Jing; Jones, Jeremy

2010-03-01

Large double stranded DNA are both a powerful system to study polymer dynamics at the single molecule level and also important molecules for genomic applications. While homogenous electric fields are routinely used to separate DNA in gels, DNA deformation in more complex fields has been less widely studied. We will demonstrate how micro/nanofluidic devices allow for the generation of electric fields with well-defined kinematics for trapping, stretching and then watching DNA relax back to equilibrium. The dimensions of the devices highly confine DNA and subsequently change both their conformation and dynamics. We will show how these confinements effects change the coil-stretch transition of a DNA being electrophoretically stretched in a purely elongational electrical field. We experimentally show that a two-stage coil stretch transition occurs and develop a simple dumbbell model which captures most of the relevant physics. We trace the origin of this phenomena to the modification of the effective spring law due to confinement.

Direct observation of λ-DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

NASA Astrophysics Data System (ADS)

Fengyun, Yang; Kaige, Wang; Dan, Sun; Wei, Zhao; Hai-qing, Wang; Xin, He; Gui-ren, Wang; Jin-tao, Bai

2016-07-01

The electrodynamic characteristics of single DNA molecules moving within micro-/nano-fluidic channels are important in the design of biomedical chips and bimolecular sensors. In this study, the dynamic properties of λ-DNA molecules transferring along the microchannels driven by the external electrickinetic force were systemically investigated with the single molecule fluorescence imaging technique. The experimental results indicated that the velocity of DNA molecules was strictly dependent on the value of the applied electric field and the diameter of the channel. The larger the external electric field, the larger the velocity, and the more significant deformation of DNA molecules. More meaningfully, it was found that the moving directions of DNA molecules had two completely different directions: (i) along the direction of the external electric field, when the electric field intensity was smaller than a certain threshold value; (ii) opposite to the direction of the external electric field, when the electric field intensity was greater than the threshold electric field intensity. The reversal movement of DNA molecules was mainly determined by the competition between the electrophoresis force and the influence of electro-osmosis flow. These new findings will theoretically guide the practical application of fluidic channel sensors and lab-on-chips for precisely manipulating single DNA molecules. Project supported by the National Natural Science Foundation of China (Grant No. 61378083), the International Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2011DFA12220), the Major Research Plan of National Natural Science Foundation of China (Grant No. 91123030), and the Natural Science Foundation of Shaanxi Province of China (Grant Nos. 2010JS110 and 2013SZS03-Z01).

Magnetic-field-induced DNA strand breaks in brain cells of the rat.

PubMed Central

Lai, Henry; Singh, Narendra P

2004-01-01

In previous research, we found that rats acutely (2 hr) exposed to a 60-Hz sinusoidal magnetic field at intensities of 0.1-0.5 millitesla (mT) showed increases in DNA single- and double-strand breaks in their brain cells. Further research showed that these effects could be blocked by pretreating the rats with the free radical scavengers melatonin and N-tert-butyl-alpha-phenylnitrone, suggesting the involvement of free radicals. In the present study, effects of magnetic field exposure on brain cell DNA in the rat were further investigated. Exposure to a 60-Hz magnetic field at 0.01 mT for 24 hr caused a significant increase in DNA single- and double-strand breaks. Prolonging the exposure to 48 hr caused a larger increase. This indicates that the effect is cumulative. In addition, treatment with Trolox (a vitamin E analog) or 7-nitroindazole (a nitric oxide synthase inhibitor) blocked magnetic-field-induced DNA strand breaks. These data further support a role of free radicals on the effects of magnetic fields. Treatment with the iron chelator deferiprone also blocked the effects of magnetic fields on brain cell DNA, suggesting the involvement of iron. Acute magnetic field exposure increased apoptosis and necrosis of brain cells in the rat. We hypothesize that exposure to a 60-Hz magnetic field initiates an iron-mediated process (e.g., the Fenton reaction) that increases free radical formation in brain cells, leading to DNA strand breaks and cell death. This hypothesis could have an important implication for the possible health effects associated with exposure to extremely low-frequency magnetic fields in the public and occupational environments. PMID:15121512

Microcomputer-assisted transmission of disaster data by cellular telephone.

PubMed

Wigder, H N; Fligner, D J; Rivers, D; Hotch, D

1989-01-01

Voice communication of information during disasters is often inadequate. In particular, simultaneous transmission by multiple callers on the same frequency can result in blocked transmissions and miscommunications. In contrast, nonvoice transmission of data requires less time than does voice communication of the same data, and may be more accurate. We conducted a pilot study to test the feasibility of a microcomputer assisted communication (MAC) network linking the disaster scene and the command hospital. The radio chosen to transmit data from the field disaster site to the command hospital was a cellular telephone connected to the microcomputer by modem. Typed communications between the microcomputer operators enabled dialogue between the disaster site and the hospitals. A computer program using commercially available software (Symphony by Lotus, Inc.) was written to allow for data entry, data transmission, and reports. Patient data, including age, sex, severity of injury, identification number, major injuries, and hospital destination were successfully transmitted from the disaster site command post to the command hospital. This pilot test demonstrated the potential applicability of MAC for facilitating transmission of patient data during a disaster.

Discrete event command and control for networked teams with multiple missions

NASA Astrophysics Data System (ADS)

Lewis, Frank L.; Hudas, Greg R.; Pang, Chee Khiang; Middleton, Matthew B.; McMurrough, Christopher

2009-05-01

During mission execution in military applications, the TRADOC Pamphlet 525-66 Battle Command and Battle Space Awareness capabilities prescribe expectations that networked teams will perform in a reliable manner under changing mission requirements, varying resource availability and reliability, and resource faults. In this paper, a Command and Control (C2) structure is presented that allows for computer-aided execution of the networked team decision-making process, control of force resources, shared resource dispatching, and adaptability to change based on battlefield conditions. A mathematically justified networked computing environment is provided called the Discrete Event Control (DEC) Framework. DEC has the ability to provide the logical connectivity among all team participants including mission planners, field commanders, war-fighters, and robotic platforms. The proposed data management tools are developed and demonstrated on a simulation study and an implementation on a distributed wireless sensor network. The results show that the tasks of multiple missions are correctly sequenced in real-time, and that shared resources are suitably assigned to competing tasks under dynamically changing conditions without conflicts and bottlenecks.

ARC-2011-ACD11-0035-013

NASA Image and Video Library

2011-02-16

New Armed Forces Reserve Center & 63rd Regional Support Command Headquarters, Moffett Field, CA ribbon cutting ceremonies and dedication of the United States Armed Forces Center is memorialized in honor of Sgt James Wilkowski.

ARC-2011-ACD11-0035-011

NASA Image and Video Library

2011-02-16

New Armed Forces Reserve Center & 63rd Regional Support Command Headquarters, Moffett Field, CA ribbon cutting ceremonies and dedication of the United States Armed Forces Center is memorialized in honor of Sgt James Wilkowski.

ARC-2011-ACD11-0035-014

NASA Image and Video Library

2011-02-16

New Armed Forces Reserve Center & 63rd Regional Support Command Headquarters, Moffett Field, CA ribbon cutting ceremonies and dedication of the United States Armed Forces Center is memorialized in honor of Sgt James Wilkowski.

ARC-2011-ACD11-0035-015

NASA Image and Video Library

2011-02-16

New Armed Forces Reserve Center & 63rd Regional Support Command Headquarters, Moffett Field, CA ribbon cutting ceremonies and dedication of the United States Armed Forces Center is memorialized in honor of Sgt James Wilkowski.

ARC-2011-ACD11-0035-017

NASA Image and Video Library

2011-02-16

New Armed Forces Reserve Center & 63rd Regional Support Command Headquarters, Moffett Field, CA ribbon cutting ceremonies and dedication of the United States Armed Forces Center is memorialized in honor of Sgt James Wilkowski.

ARC-2011-ACD11-0035-018

NASA Image and Video Library

2011-02-16

New Armed Forces Reserve Center & 63rd Regional Support Command Headquarters, Moffett Field, CA ribbon cutting ceremonies and dedication of the United States Armed Forces Center is memorialized in honor of Sgt James Wilkowski.

ARC-2011-ACD11-0035-016

NASA Image and Video Library

2011-02-16

New Armed Forces Reserve Center & 63rd Regional Support Command Headquarters, Moffett Field, CA ribbon cutting ceremonies and dedication of the United States Armed Forces Center is memorialized in honor of Sgt James Wilkowski.

32 CFR 165.2 - Applicability and scope.

Code of Federal Regulations, 2010 CFR

2010-07-01

... the Joint Staff, the Unified and Specified Commands, the Defense Agencies, and the DoD Field... activities and the property is sold in open competition to the highest bidder. (c) The policies and...

Nano-funnels as electro-osmotic ``tweezers and pistons''

NASA Astrophysics Data System (ADS)

Wang, Yanqian; Panyukov, Sergey; Zhou, Jinsheng; Menard, Laurent D.; Ramsey, J. Michael; Rubinstien, Michael

2014-03-01

An electric field is used to force a DNA molecule into a nano-channel by compensating the free energy penalty that results from the reduced conformational entropy of the confined macromolecule. Narrow nano-channels require high critical electric fields to achieve DNA translocation, leading to short dwell times of DNA in these channels. We demonstrate that nano-funnels integrated with nano-channels reduce the free energy barrier and lower the critical electric field required for DNA translocation. A focused electric field within the funnel increases the electric force on the DNA, compresses the molecule, and increases the osmotic pressure at the nano-channel entrance. This ``electro-osmotic piston'' forces the molecule into the nano-channel at lower electric fields than those observed without the funnel. Appropirately designed nano-funnels can also function as tweezers that allow manipulation of the position of the DNA molecule. The predictions of our theory describing double-stranded DNA behavior in nano-funnel - nano-channel devices are consistent with experimental results. Thanks for the financial support from NSF (DMR-1309892, DMR-1121107, DMR-1122483), NIH (1-P50-HL107168, 1-P01-HL108808-01A1, R01HG02647), NHGRI and CF Foundation.

ASSTC and field sensors: new technology for emergency care

NASA Astrophysics Data System (ADS)

Morrison, G. Wayne; Vo-Dinh, Tuan

2000-05-01

The US Army Medical Research and Material Command together with the US Marine Corps Combat Development Command sponsored the design and production of a far-forward, lightweight, small footprint, reconfigurable, highly mobile Advanced Surgical Suite for Trauma Casualties (ASSTC) to reduce combat casualties and morbidity. The KIA fraction has remained relatively constant over major wars and conflicts since the early 1900s. One third of the KIA perish after 10 minutes. ASSTC has the potential to dramatically lower this fraction by providing resuscitative care within a short period of wound infliction and not requiring long transport times to the caregivers. ASSTC is also unique in its capability to serve in multiple missions including humanitarian aid, infectious disease control, and disaster relief. Adding field sensor to ASSTC greatly enhances the capability of this highly mobile system to operate in many areas.

DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer.

PubMed

Bronder, Thomas S; Poghossian, Arshak; Scheja, Sabrina; Wu, Chunsheng; Keusgen, Michael; Mewes, Dieter; Schöning, Michael J

2015-09-16

Miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge favor the semiconductor field-effect platform as one of the most attractive approaches for the development of label-free DNA chips. In this work, a capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with a layer-by-layer prepared, positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was used for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization. The negatively charged probe single-stranded DNA (ssDNA) molecules were electrostatically adsorbed onto the positively charged PAH layer, resulting in a preferentially flat orientation of the ssDNA molecules within the Debye length, thus yielding a reduced charge-screening effect and a higher sensor signal. Each sensor-surface modification step (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), reducing an unspecific adsorption by a blocking agent, incubation with noncomplementary DNA (ncDNA) solution) was monitored by means of capacitance-voltage and constant-capacitance measurements. In addition, the surface morphology of the PAH layer was studied by atomic force microscopy and contact-angle measurements. High hybridization signals of 34 and 43 mV were recorded in low-ionic strength solutions of 10 and 1 mM, respectively. In contrast, a small signal of 4 mV was recorded in the case of unspecific adsorption of fully mismatched ncDNA. The density of probe ssDNA and dsDNA molecules as well as the hybridization efficiency was estimated using the experimentally measured DNA immobilization and hybridization signals and a simplified double-layer capacitor model. The results of field-effect experiments were supported by fluorescence measurements, verifying the DNA-immobilization and hybridization event.

STS-27 crewmembers walk across parking apron to T-38s at Ellington Field

NASA Technical Reports Server (NTRS)

1988-01-01

STS-27 Atlantis, Orbiter Vehicle (OV) 104, crewmembers walk toward the flight line of T-38s at Ellington Field prior to their departure for Kennedy Space Center (KSC). Left to right are Mission Specialist (MS) Jerry L. Ross, Pilot Guy S. Gardner, MS William M. Shepherd, and Commander Robert L. Gibson.

STS-109 Crew Return Ceremony at Ellington Field

NASA Image and Video Library

2002-03-13

JSC2002-E-09329 (13 March 2002) --- Astronaut Duane G. Carey (right foreground), STS-109 pilot, shakes hands with Johnson Space Center’s (JSC) Acting Director Roy Estess during the crew return ceremonies at Ellington Field. Also pictured are astronaut Scott D. Altman (left background), mission commander, and astronaut Steven A. Hawley, Director of Flight Crew Operations.

Polarizable Force Field for DNA Based on the Classical Drude Oscillator: I. Refinement Using Quantum Mechanical Base Stacking and Conformational Energetics.

PubMed

Lemkul, Justin A; MacKerell, Alexander D

2017-05-09

Empirical force fields seek to relate the configuration of a set of atoms to its energy, thus yielding the forces governing its dynamics, using classical physics rather than more expensive quantum mechanical calculations that are computationally intractable for large systems. Most force fields used to simulate biomolecular systems use fixed atomic partial charges, neglecting the influence of electronic polarization, instead making use of a mean-field approximation that may not be transferable across environments. Recent hardware and software developments make polarizable simulations feasible, and to this end, polarizable force fields represent the next generation of molecular dynamics simulation technology. In this work, we describe the refinement of a polarizable force field for DNA based on the classical Drude oscillator model by targeting quantum mechanical interaction energies and conformational energy profiles of model compounds necessary to build a complete DNA force field. The parametrization strategy employed in the present work seeks to correct weak base stacking in A- and B-DNA and the unwinding of Z-DNA observed in the previous version of the force field, called Drude-2013. Refinement of base nonbonded terms and reparametrization of dihedral terms in the glycosidic linkage, deoxyribofuranose rings, and important backbone torsions resulted in improved agreement with quantum mechanical potential energy surfaces. Notably, we expand on previous efforts by explicitly including Z-DNA conformational energetics in the refinement.

32 CFR 806b.39 - Requesting an exemption.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Air Force Chief Information Officer/P through the Major Command or Field Operating Agency Privacy Act Officer. The request will detail the reasons for the exemption, the section of the Act that allows the...

32 CFR 806b.39 - Requesting an exemption.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Air Force Chief Information Officer/P through the Major Command or Field Operating Agency Privacy Act Officer. The request will detail the reasons for the exemption, the section of the Act that allows the...

32 CFR 806b.39 - Requesting an exemption.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Air Force Chief Information Officer/P through the Major Command or Field Operating Agency Privacy Act Officer. The request will detail the reasons for the exemption, the section of the Act that allows the...

32 CFR 806b.39 - Requesting an exemption.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Air Force Chief Information Officer/P through the Major Command or Field Operating Agency Privacy Act Officer. The request will detail the reasons for the exemption, the section of the Act that allows the...

32 CFR 806b.39 - Requesting an exemption.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Air Force Chief Information Officer/P through the Major Command or Field Operating Agency Privacy Act Officer. The request will detail the reasons for the exemption, the section of the Act that allows the...

OSG-GEM: Gene Expression Matrix Construction Using the Open Science Grid.

PubMed

Poehlman, William L; Rynge, Mats; Branton, Chris; Balamurugan, D; Feltus, Frank A

2016-01-01

High-throughput DNA sequencing technology has revolutionized the study of gene expression while introducing significant computational challenges for biologists. These computational challenges include access to sufficient computer hardware and functional data processing workflows. Both these challenges are addressed with our scalable, open-source Pegasus workflow for processing high-throughput DNA sequence datasets into a gene expression matrix (GEM) using computational resources available to U.S.-based researchers on the Open Science Grid (OSG). We describe the usage of the workflow (OSG-GEM), discuss workflow design, inspect performance data, and assess accuracy in mapping paired-end sequencing reads to a reference genome. A target OSG-GEM user is proficient with the Linux command line and possesses basic bioinformatics experience. The user may run this workflow directly on the OSG or adapt it to novel computing environments.

OSG-GEM: Gene Expression Matrix Construction Using the Open Science Grid

PubMed Central

Poehlman, William L.; Rynge, Mats; Branton, Chris; Balamurugan, D.; Feltus, Frank A.

2016-01-01

High-throughput DNA sequencing technology has revolutionized the study of gene expression while introducing significant computational challenges for biologists. These computational challenges include access to sufficient computer hardware and functional data processing workflows. Both these challenges are addressed with our scalable, open-source Pegasus workflow for processing high-throughput DNA sequence datasets into a gene expression matrix (GEM) using computational resources available to U.S.-based researchers on the Open Science Grid (OSG). We describe the usage of the workflow (OSG-GEM), discuss workflow design, inspect performance data, and assess accuracy in mapping paired-end sequencing reads to a reference genome. A target OSG-GEM user is proficient with the Linux command line and possesses basic bioinformatics experience. The user may run this workflow directly on the OSG or adapt it to novel computing environments. PMID:27499617

A convenient and adaptable package of DNA sequence analysis programs for microcomputers.

PubMed Central

Pustell, J; Kafatos, F C

1982-01-01

We describe a package of DNA data handling and analysis programs designed for microcomputers. The package is convenient for immediate use by persons with little or no computer experience, and has been optimized by trial in our group for a year. By typing a single command, the user enters a system which asks questions or gives instructions in English. The system will enter, alter, and manage sequence files or a restriction enzyme library. It generates the reverse complement, translates, calculates codon usage, finds restriction sites, finds homologies with various degrees of mismatch, and graphs amino acid composition or base frequencies. A number of options for data handling and printing can be used to produce figures for publication. The package will be available in ANSI Standard FORTRAN for use with virtually any FORTRAN compiler. PMID:6278412

DSSR-enhanced visualization of nucleic acid structures in Jmol

PubMed Central

Hanson, Robert M.

2017-01-01

Abstract Sophisticated and interactive visualizations are essential for making sense of the intricate 3D structures of macromolecules. For proteins, secondary structural components are routinely featured in molecular graphics visualizations. However, the field of RNA structural bioinformatics is still lagging behind; for example, current molecular graphics tools lack built-in support even for base pairs, double helices, or hairpin loops. DSSR (Dissecting the Spatial Structure of RNA) is an integrated and automated command-line tool for the analysis and annotation of RNA tertiary structures. It calculates a comprehensive and unique set of features for characterizing RNA, as well as DNA structures. Jmol is a widely used, open-source Java viewer for 3D structures, with a powerful scripting language. JSmol, its reincarnation based on native JavaScript, has a predominant position in the post Java-applet era for web-based visualization of molecular structures. The DSSR-Jmol integration presented here makes salient features of DSSR readily accessible, either via the Java-based Jmol application itself, or its HTML5-based equivalent, JSmol. The DSSR web service accepts 3D coordinate files (in mmCIF or PDB format) initiated from a Jmol or JSmol session and returns DSSR-derived structural features in JSON format. This seamless combination of DSSR and Jmol/JSmol brings the molecular graphics of 3D RNA structures to a similar level as that for proteins, and enables a much deeper analysis of structural characteristics. It fills a gap in RNA structural bioinformatics, and is freely accessible (via the Jmol application or the JSmol-based website http://jmol.x3dna.org). PMID:28472503

Trapping and breaking of in vivo nicked DNA during pulsed-field gel electrophoresis

PubMed Central

Khan, Sharik R.; Kuzminov, Andrei

2013-01-01

Pulsed field gel electrophoresis (PFGE) offers a high-resolution approach to quantify chromosomal fragmentation in bacteria, measured as percent of chromosomal DNA entering the gel. The degree of separation in PFG depends upon the size of DNA, as well as various conditions of electrophoresis, such as electric field strength (FS), time of electrophoresis, switch time and buffer composition. Here we describe a new parameter, the structural integrity of the sample DNA itself, that influences its migration through PFGs. We show that sub-chromosomal fragments containing both spontaneous and DNA damage-induced nicks are prone to breakage during PFGE. Such breakage at single strand interruptions results in artefactual decrease in molecular weight of linear DNA making accurate determination of the number of double strand breaks difficult. While breakage of nicked sub-chromosomal fragments is FS-independent, some high molecular weight sub-chromosomal fragments are also trapped within wells under the standard PFGE conditions. This trapping can be minimized by lowering the field strength and increasing the time of electrophoresis. We discuss how breakage of nicked DNA may be mechanistically linked to trapping. Our results suggest how to optimize conditions for PFGE when quantifying chromosomal fragmentation induced by DNA damage. PMID:23770235

Electrostatic field of the large fragment of Escherichia coli DNA polymerase I.

PubMed

Warwicker, J; Ollis, D; Richards, F M; Steitz, T A

1985-12-05

The electrostatic field of the large fragment of Escherichia coli DNA polymerase I (Klenow fragment) has been calculated by the finite difference procedure on a 2 A grid. The potential field is substantially negative at physiological pH (reflecting the net negative charge at this pH). The largest regions of positive potential are in the deep crevice of the C-terminal domain, which is the proposed binding site for the DNA substrate. Within the crevice, the electrostatic potential has a partly helical form. If the DNA is positioned to fulfil stereochemical requirements, then the positive potential generally follows the major groove and (to a lesser extent) the negative potential is in the minor groove. Such an arrangement could stabilize DNA configurations related by screw symmetry. The histidine residues of the Klenow fragment give the positive field of the groove a sensitivity to relatively small pH changes around neutrality. We suggest that the histidine residues could change their ionization states in response to DNA binding, and that this effect could contribute to the protein-DNA binding energy.

Team Leader Structuring for Team Effectiveness and Team Learning in Command-and-Control Teams.

PubMed

van der Haar, Selma; Koeslag-Kreunen, Mieke; Euwe, Eline; Segers, Mien

2017-04-01

Due to their crucial and highly consequential task, it is of utmost importance to understand the levers leading to effectiveness of multidisciplinary emergency management command-and-control (EMCC) teams. We argue that the formal EMCC team leader needs to initiate structure in the team meetings to support organizing the work as well as facilitate team learning, especially the team learning process of constructive conflict. In a sample of 17 EMCC teams performing a realistic EMCC exercise, including one or two team meetings (28 in sum), we coded the team leader's verbal structuring behaviors (1,704 events), rated constructive conflict by external experts, and rated team effectiveness by field experts. Results show that leaders of effective teams use structuring behaviors more often (except asking procedural questions) but decreasingly over time. They support constructive conflict by clarifying and by making summaries that conclude in a command or decision in a decreasing frequency over time.

Apollo 16 lunar module "Orion" photographed from distance during EVA

NASA Image and Video Library

1972-04-23

AS16-116-18678 (23 April 1972) --- A view from the moving Apollo 16 Lunar Roving Vehicle (LRV) as the crew men headed "home" at the end of the mission's third and final extravehicular activity (EVA). Astronaut John W. Young called attention to the series of block fields between the Lunar Module (LM) and LRV. Young also noted that, "The LM was obviously sitting in the only flat place around." Stone Mountain stretches about half way across the background. The high gain antenna and the RCA television camera on the LRV are in the foreground. While astronauts Young, commander; and Charles M. Duke Jr., lunar module pilot; descended in the Apollo 16 LM "Orion" to explore the Descartes highlands landing site on the moon, astronaut Thomas K. Mattingly II, command module pilot, remained with the Command and Service Modules (CSM) "Casper" in lunar orbit.

Cloud Geometry Analysis of the Smoke Week III Obscuration Trials.

DTIC Science & Technology

1982-01-01

GRAPIC 1LJu 4 Cf . .444,44 44- 0. 15, 25, TltME( SErs AOEDET. . **** *HEI HT0F CENTER OP MASS ABOVE DET. PT 42 SMOKE III EVENT # 07 1313 Z 08-12-80 STATION...PORTION OF CLOUD ATMOSPHEdIC SCIENCES LABORATORY WHITE SANDS MISSILE RANGE, N.M. 119 CLI-J000000 ) .0 0 1a 40 M M M MMMMMMMM ul zo w -ZIxJ z z w 0 L- CF ... CF -R (CPT James M. Watson) Dugway, UT 84022 Port Sill, OK 73503 Commander Commandant US Army Dugway Proving Ground US Army Field Artillery School ATTN

Commander Mattingly prepares meal on middeck

NASA Image and Video Library

1982-07-04

STS004-28-312 (27 June-4 July 1982) --- Astronaut Thomas K. Mattingly II, STS-4 crew commander, prepares a meal in the middeck area of space shuttle Columbia. He uses scissors to open a drink container. Various packages of food and meal accessories are attached to locker doors. At far left edge of the frame is the tall payload called continuous flow electrophoresis experiment (CFES) system-designed to separate biological materials according to their surface electrical charges as they pass through an electrical field. Astronaut Henry W. Hartsfield Jr. exposed this frame with a 35mm camera. Photo credit: NASA

DNA nanotechnology

NASA Astrophysics Data System (ADS)

Seeman, Nadrian C.; Sleiman, Hanadi F.

2018-01-01

DNA is the molecule that stores and transmits genetic information in biological systems. The field of DNA nanotechnology takes this molecule out of its biological context and uses its information to assemble structural motifs and then to connect them together. This field has had a remarkable impact on nanoscience and nanotechnology, and has been revolutionary in our ability to control molecular self-assembly. In this Review, we summarize the approaches used to assemble DNA nanostructures and examine their emerging applications in areas such as biophysics, diagnostics, nanoparticle and protein assembly, biomolecule structure determination, drug delivery and synthetic biology. The introduction of orthogonal interactions into DNA nanostructures is discussed, and finally, a perspective on the future directions of this field is presented.

Recovery Based Nanowire Field-Effect Transistor Detection of Pathogenic Avian Influenza DNA

NASA Astrophysics Data System (ADS)

Lin, Chih-Heng; Chu, Chia-Jung; Teng, Kang-Ning; Su, Yi-Jr; Chen, Chii-Dong; Tsai, Li-Chu; Yang, Yuh-Shyong

2012-02-01

Fast and accurate diagnosis is critical in infectious disease surveillance and management. We proposed a DNA recovery system that can easily be adapted to DNA chip or DNA biosensor for fast identification and confirmation of target DNA. This method was based on the re-hybridization of DNA target with a recovery DNA to free the DNA probe. Functionalized silicon nanowire field-effect transistor (SiNW FET) was demonstrated to monitor such specific DNA-DNA interaction using high pathogenic strain virus hemagglutinin 1 (H1) DNA of avian influenza (AI) as target. Specific electric changes were observed in real-time for AI virus DNA sensing and device recovery when nanowire surface of SiNW FET was modified with complementary captured DNA probe. The recovery based SiNW FET biosensor can be further developed for fast identification and further confirmation of a variety of influenza virus strains and other infectious diseases.

Rapid concentration of deoxyribonucleic acid via Joule heating induced temperature gradient focusing in poly-dimethylsiloxane microfluidic channel.

PubMed

Ge, Zhengwei; Wang, Wei; Yang, Chun

2015-02-09

This paper reports rapid microfluidic electrokinetic concentration of deoxyribonucleic acid (DNA) with the Joule heating induced temperature gradient focusing (TGF) by using our proposed combined AC and DC electric field technique. A peak of 480-fold concentration enhancement of DNA sample is achieved within 40s in a simple poly-dimethylsiloxane (PDMS) microfluidic channel of a sudden expansion in cross-section. Compared to a sole DC field, the introduction of an AC field can reduce DC field induced back-pressure and produce sufficient Joule heating effects, resulting in higher concentration enhancement. Within such microfluidic channel structure, negative charged DNA analytes can be concentrated at a location where the DNA electrophoretic motion is balanced with the bulk flow driven by DC electroosmosis under an appropriate temperature gradient field. A numerical model accounting for a combined AC and DC field and back-pressure driven flow effects is developed to describe the complex Joule heating induced TGF processes. The experimental observation of DNA concentration phenomena can be explained by the numerical model. Copyright © 2014 Elsevier B.V. All rights reserved.

STS-104 Crew Return, Ellington Field, Building 990

NASA Image and Video Library

2001-07-26

JSC2001-E-22794 (25 July 2001) --- Astronaut Steven W. Lindsey, STS-104 mission commander, addresses a crowd at Ellington Field’s Hangar 990 for a crew return ceremony. Seated from the left are Michael L. Gernhardt, James F. Reilly, both mission specialist, and Roy S. Estess, JSC Acting Director, along with Janet L. Kavandi, mission specialist, and Charles O. Hobaugh, pilot.

Photographic documentation of the return of the STS-98 crew to Ellington Field

NASA Image and Video Library

2001-02-22

JSC2001-E-04804 (21 February 2001) --- Astronauts Mark L. Polansky (left) and Kenneth D. Cockrell (center), pilot and commander, respectively, for the STS-98 mission, are greeted by Steven A. Hawley of the Flight Crew Operations Directorate upon their return to Houston. The greetings took place prior to a welcoming ceremony at Ellington Field.

Effect of a magnetic field on the track structure of low-energy electrons: a Monte Carlo study

NASA Astrophysics Data System (ADS)

Bug, M. U.; Gargioni, E.; Guatelli, S.; Incerti, S.; Rabus, H.; Schulte, R.; Rosenfeld, A. B.

2010-10-01

The increasing use of MRI-guided radiation therapy evokes the necessity to investigate the potential impact of a magnetic field on the biological effectiveness of therapeutic radiation beams. While it is known that a magnetic field, applied during irradiation, can improve the macroscopic absorbed dose distribution of electrons in the tumor region, effects on the microscopic distribution of energy depositions and ionizations have not yet been investigated. An effect on the number of ionizations in a DNA segment, which is related to initial DNA damage in form of complex strand breaks, could be beneficial in radiation therapy. In this work we studied the effects of a magnetic field on the pattern of ionizations at nanometric level by means of Monte Carlo simulations using the Geant4-DNA toolkit. The track structure of low-energy electrons in the presence of a uniform static magnetic field of strength up to 14 T was calculated for a simplified DNA segment model in form of a water cylinder. In the case that no magnetic field is applied, nanodosimetric results obtained with Geant4-DNA were compared with those from the PTB track structure code. The obtained results suggest that any potential enhancement of complexity of DNA strand breaks induced by irradiation in a magnetic field is not related to modifications of the low-energy secondary electrons track structure.

The Volume Grid Manipulator (VGM): A Grid Reusability Tool

NASA Technical Reports Server (NTRS)

Alter, Stephen J.

1997-01-01

This document is a manual describing how to use the Volume Grid Manipulation (VGM) software. The code is specifically designed to alter or manipulate existing surface and volume structured grids to improve grid quality through the reduction of grid line skewness, removal of negative volumes, and adaption of surface and volume grids to flow field gradients. The software uses a command language to perform all manipulations thereby offering the capability of executing multiple manipulations on a single grid during an execution of the code. The command language can be input to the VGM code by a UNIX style redirected file, or interactively while the code is executing. The manual consists of 14 sections. The first is an introduction to grid manipulation; where it is most applicable and where the strengths of such software can be utilized. The next two sections describe the memory management and the manipulation command language. The following 8 sections describe simple and complex manipulations that can be used in conjunction with one another to smooth, adapt, and reuse existing grids for various computations. These are accompanied by a tutorial section that describes how to use the commands and manipulations to solve actual grid generation problems. The last two sections are a command reference guide and trouble shooting sections to aid in the use of the code as well as describe problems associated with generated scripts for manipulation control.

The specific host plant DNA detection suggests a potential migration of Apolygus lucorum from cotton to mungbean fields

PubMed Central

Bao, Wei-Fang; Yang, Fan; Xu, Bin; Yang, Yi-Zhong

2017-01-01

The polyphagous mirid bug Apolygus lucorum (Heteroptera: Miridae) has more than 200 species of host plants and is an insect pest of important agricultural crops, including cotton (Gossypium hirsutum) and mungbean (Vigna radiata). Previous field trials have shown that A. lucorum adults prefer mungbean to cotton plants, indicating the considerable potential of mungbean as a trap crop in cotton fields. However, direct evidence supporting the migration of A. lucorum adults from cotton to mungbean is lacking. We developed a DNA-based polymerase chain reaction (PCR) approach to reveal the movement of A. lucorum between neighboring mungbean and cotton fields. Two pairs of PCR primers specific to cotton or mungbean were designed to target the trnL-trnF region of chloroplast DNA. Significant differences in the detectability half-life (DS50) were observed between these two host plants, and the mean for cotton (8.26 h) was approximately two times longer than that of mungbean (4.38 h), requiring weighted mean calculations to compare the detectability of plant DNA in the guts of field-collected bugs. In field trials, cotton DNA was detected in the guts of the adult A. lucorum individuals collected in mungbean plots, and the cotton DNA detection rate decreased successively from 5 to 15 m away from the mungbean-cotton midline. In addition to the specific detection of cotton- and mungbean-fed bugs, both cotton and mungbean DNA were simultaneously detected within the guts of single individuals caught from mungbean fields. This study successfully established a tool for molecular gut-content analyses and clearly demonstrated the movement of A. lucorum adults from cotton to neighboring mungbean fields, providing new insights into understanding the feeding characteristics and landscape-level ecology of A. lucorum under natural conditions. PMID:28586352

32 CFR 226.2 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS... Defense (OSD), the Military Departments (including their National Guard and Reserve components), the Unified and Specified Commands, the Defense Agencies, and Department of Defense Field Activities...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sierra Thermal/Fluid Team

SIERRA/Aero is a compressible fluid dynamics program intended to solve a wide variety compressible fluid flows including transonic and hypersonic problems. This document describes the commands for assembling a fluid model for analysis with this module, henceforth referred to simply as Aero for brevity. Aero is an application developed using the SIERRA Toolkit (STK). The intent of STK is to provide a set of tools for handling common tasks that programmers encounter when developing a code for numerical simulation. For example, components of STK provide field allocation and management, and parallel input/output of field and mesh data. These services alsomore » allow the development of coupled mechanics analysis software for a massively parallel computing environment. In the definitions of the commands that follow, the term Real_Max denotes the largest floating point value that can be represented on a given computer. Int_Max is the largest such integer value.« less

FootFall: A Ground Based Operations Toolset Enabling Walking for the ATHLETE Rover

NASA Technical Reports Server (NTRS)

SunSpiral, Vytas; Chavez-Clemente, Daniel; Broxton, Michael; Keely, Leslie; Mihelich, Patrick; Mittman, David; Collins, Curtis

2008-01-01

The ATHLETE (All-Terrain Hex-Limbed Extra-Terrestrial Explorer) vehicle consists of six identical, six degree of freedom limbs. FootFall is a ground tool for ATHLETE intended to provide an operator with integrated situational awareness, terrain reconstruction, stability and safety analysis, motion planning, and decision support capabilities to enable the efficient generation of flight software command sequences for walking. FootFall has been under development at NASA Ames for the last year, and having accomplished the initial integration, it is being used to generate command sequences for single footfalls. In this paper, the architecture of FootFall in its current state will be presented, results from the recent Human Robotic Systems Project?s Integrated Field Test (Moses Lake, Washington, June, 2008) will be discussed, and future plans for extending the capabilities of FootFall to enable ATHLETE to walk across a boulder field in real time will be described.

Nedd4 Family Interacting Protein 1 (Ndfip1) Is Required for Ubiquitination and Nuclear Trafficking of BRCA1-associated ATM Activator 1 (BRAT1) during the DNA Damage Response*

PubMed Central

Low, Ley-Hian; Chow, Yuh-Lit; Li, Yijia; Goh, Choo-Peng; Putz, Ulrich; Silke, John; Ouchi, Toru; Howitt, Jason; Tan, Seong-Seng

2015-01-01

During injury, cells are vulnerable to apoptosis from a variety of stress conditions including DNA damage causing double-stranded breaks. Without repair, these breaks lead to aberrations in DNA replication and transcription, leading to apoptosis. A major response to DNA damage is provided by the protein kinase ATM (ataxia telangiectasia mutated) that is capable of commanding a plethora of signaling networks for DNA repair, cell cycle arrest, and even apoptosis. A key element in the DNA damage response is the mobilization of activating proteins into the cell nucleus to repair damaged DNA. BRAT1 is one of these proteins, and it functions as an activator of ATM by maintaining its phosphorylated status while also keeping other phosphatases at bay. However, it is unknown how BRAT1 is trafficked into the cell nucleus to maintain ATM phosphorylation. Here we demonstrate that Ndfip1-mediated ubiquitination of BRAT1 leads to BRAT1 trafficking into the cell nucleus. Without Ndfip1, BRAT1 failed to translocate to the nucleus. Under genotoxic stress, cells showed increased expression of both Ndfip1 and phosphorylated ATM. Following brain injury, neurons show increased expression of Ndfip1 and nuclear translocation of BRAT1. These results point to Ndfip1 as a sensor protein during cell injury and Ndfip1 up-regulation as a cue for BRAT1 ubiquitination by Nedd4 E3 ligases, followed by nuclear translocation of BRAT1. PMID:25631046

DNA Nanotechnology for Cancer Therapy

PubMed Central

Kumar, Vinit; Palazzolo, Stefano; Bayda, Samer; Corona, Giuseppe; Toffoli, Giuseppe; Rizzolio, Flavio

2016-01-01

DNA nanotechnology is an emerging and exciting field, and represents a forefront frontier for the biomedical field. The specificity of the interactions between complementary base pairs makes DNA an incredible building material for programmable and very versatile two- and three-dimensional nanostructures called DNA origami. Here, we analyze the DNA origami and DNA-based nanostructures as a drug delivery system. Besides their physical-chemical nature, we dissect the critical factors such as stability, loading capability, release and immunocompatibility, which mainly limit in vivo applications. Special attention was dedicated to highlighting the boundaries to be overcome to bring DNA nanostructures closer to the bedside of patients. PMID:27022418

1,25-Dihydroxyvitamin D3 Suppresses TLR8 Expression and TLR8-Mediated Inflammatory Responses in Monocytes In Vitro and Experimental Autoimmune Encephalomyelitis In Vivo

DTIC Science & Technology

2013-03-14

was supported by funding from the Telemedicine and Advanced Technology Research Center (TATRC) at the US Army Medical Research and Materiel Command...instructions. Specific primers were synthesized by Integrated DNA Technologies (San Diego, CA) as shown in Table S1. Real-time PCR was performed with SYBR...PFA for 1 h at 4uC and then permeablized in a buffer containing 0.1% saponin . Nonspecific antibody binding to Fc receptors was blocked by incubation

Autonomous Image Analysis for Future Mars Missions

NASA Technical Reports Server (NTRS)

Gulick, V. C.; Morris, R. L.; Ruzon, M. A.; Bandari, E.; Roush, T. L.

1999-01-01

To explore high priority landing sites and to prepare for eventual human exploration, future Mars missions will involve rovers capable of traversing tens of kilometers. However, the current process by which scientists interact with a rover does not scale to such distances. Specifically, numerous command cycles are required to complete even simple tasks, such as, pointing the spectrometer at a variety of nearby rocks. In addition, the time required by scientists to interpret image data before new commands can be given and the limited amount of data that can be downlinked during a given command cycle constrain rover mobility and achievement of science goals. Experience with rover tests on Earth supports these concerns. As a result, traverses to science sites as identified in orbital images would require numerous science command cycles over a period of many weeks, months or even years, perhaps exceeding rover design life and other constraints. Autonomous onboard science analysis can address these problems in two ways. First, it will allow the rover to preferentially transmit "interesting" images, defined as those likely to have higher science content. Second, the rover will be able to anticipate future commands. For example, a rover might autonomously acquire and return spectra of "interesting" rocks along with a high-resolution image of those rocks in addition to returning the context images in which they were detected. Such approaches, coupled with appropriate navigational software, help to address both the data volume and command cycle bottlenecks that limit both rover mobility and science yield. We are developing fast, autonomous algorithms to enable such intelligent on-board decision making by spacecraft. Autonomous algorithms developed to date have the ability to identify rocks and layers in a scene, locate the horizon, and compress multi-spectral image data. We are currently investigating the possibility of reconstructing a 3D surface from a sequence of images acquired by a robotic arm camera. This would then allow the return of a single completely in focus image constructed only from those portions of individual images that lie within the camera's depth of field. Output from these algorithms could be used to autonomously obtain rock spectra, determine which images should be transmitted to the ground, or to aid in image compression. We will discuss these algorithms and their performance during a recent rover field test.

Operation UPSHOT-KNOTHOLE. Operational Summary, Nevada Proving Grounds, 1 March - 9 June 1953

DTIC Science & Technology

1982-08-01

EXTRACTED VERSION OPERATION UPSHOT-KNOTHOLE -- Operational Summary Nevada Proving Grounds ,* 1 March-9 June 1953 Headquarters Field Command Armed...Weapons Effects Tests 9. PERIORUMING 004CAWIZAY10ON AME ANO AOO1RESS 10. P140GRAM ELEMENPT, PROJECT, TASK AREA a 1114.01110 UNIT NUMBERS Headquarters Field ...process of preparation. Activities in prepara- tion for the operational period have been previously recorded in Field Commnand Histo y DO I "’* 1473

A Low-Power Wearable Stand-Alone Tongue Drive System for People With Severe Disabilities.

PubMed

Jafari, Ali; Buswell, Nathanael; Ghovanloo, Maysam; Mohsenin, Tinoosh

2018-02-01

This paper presents a low-power stand-alone tongue drive system (sTDS) used for individuals with severe disabilities to potentially control their environment such as computer, smartphone, and wheelchair using their voluntary tongue movements. A low-power local processor is proposed, which can perform signal processing to convert raw magnetic sensor signals to user-defined commands, on the sTDS wearable headset, rather than sending all raw data out to a PC or smartphone. The proposed sTDS significantly reduces the transmitter power consumption and subsequently increases the battery life. Assuming the sTDS user issues one command every 20 ms, the proposed local processor reduces the data volume that needs to be wirelessly transmitted by a factor of 64, from 9.6 to 0.15 kb/s. The proposed processor consists of three main blocks: serial peripheral interface bus for receiving raw data from magnetic sensors, external magnetic interference attenuation to attenuate external magnetic field from the raw magnetic signal, and a machine learning classifier for command detection. A proof-of-concept prototype sTDS has been implemented with a low-power IGLOO-nano field programmable gate array (FPGA), bluetooth low energy, battery and magnetic sensors on a headset, and tested. At clock frequency of 20 MHz, the processor takes 6.6 s and consumes 27 nJ for detecting a command with a detection accuracy of 96.9%. To further reduce power consumption, an application-specified integrated circuit processor for the sTDS is implemented at the postlayout level in 65-nm CMOS technology with 1-V power supply, and it consumes 0.43 mW, which is 10 lower than FPGA power consumption and occupies an area of only 0.016 mm.

The effects of time delays on a telepathology user interface.

PubMed Central

Carr, D.; Hasegawa, H.; Lemmon, D.; Plaisant, C.

1992-01-01

Telepathology enables a pathologist to examine physically distant tissue samples by microscope operation over a communication link. Communication links can impose time delays which cause difficulties in controlling the remote device. Such difficulties were found in a microscope teleoperation system. Since the user interface is critical to pathologist's acceptance of telepathology, we redesigned the user interface for this system, built two different versions (a keypad whose movement commands operated by specifying a start command followed by a stop command and a trackball interface whose movement commands were incremental and directly proportional to the rotation of the trackball). We then conducted a pilot study to determine the effect of time delays on the new user interfaces. In our experiment, the keypad was the faster interface when the time delay is short. There was no evidence to favor either the keypad or trackball when the time delay was longer. Inexperienced participants benefitted by allowing them to move long distances over the microscope slide by dragging the field-of-view indicator on the touchscreen control panel. The experiment suggests that changes could be made to the trackball interface which would improve its performance. PMID:1482878

Chondroitin sulfate-polyethylenimine copolymer-coated superparamagnetic iron oxide nanoparticles as an efficient magneto-gene carrier for microRNA-encoding plasmid DNA delivery

NASA Astrophysics Data System (ADS)

Lo, Yu-Lun; Chou, Han-Lin; Liao, Zi-Xian; Huang, Shih-Jer; Ke, Jyun-Han; Liu, Yu-Sheng; Chiu, Chien-Chih; Wang, Li-Fang

2015-04-01

MicroRNA-128 (miR-128) is an attractive therapeutic molecule with powerful glioblastoma regulation properties. However, miR-128 lacks biological stability and leads to poor delivery efficacy in clinical applications. In our previous study, we demonstrated two effective transgene carriers, including polyethylenimine (PEI)-decorated superparamagnetic iron oxide nanoparticles (SPIONs) as well as chemically-conjugated chondroitin sulfate-PEI copolymers (CPs). In this contribution, we report optimized conditions for coating CPs onto the surfaces of SPIONs, forming CPIOs, for magneto-gene delivery systems. The optimized weight ratio of the CPs and SPIONs is 2 : 1, which resulted in the formation of a stable particle as a good transgene carrier. The hydrodynamic diameter of the CPIOs is ~136 nm. The gel electrophoresis results demonstrate that the weight ratio of CPIO/DNA required to completely encapsulate pDNA is >=3. The in vitro tests of CPIO/DNA were done in 293 T, CRL5802, and U87-MG cells in the presence and absence of an external magnetic field. The magnetofection efficiency of CPIO/DNA was measured in the three cell lines with or without fetal bovine serum (FBS). CPIO/DNA exhibited remarkably improved gene expression in the presence of the magnetic field and 10% FBS as compared with a gold non-viral standard, PEI/DNA, and a commercial magnetofection reagent, PolyMag/DNA. In addition, CPIO/DNA showed less cytotoxicity than PEI/DNA and PolyMag/DNA against the three cell lines. The transfection efficiency of the magnetoplex improved significantly with an assisted magnetic field. In miR-128 delivery, a microRNA plate array and fluorescence in situ hybridization were used to demonstrate that CPIO/pMIRNA-128 indeed expresses more miR-128 with the assisted magnetic field than without. In a biodistribution test, CPIO/Cy5-DNA showed higher accumulation at the tumor site where an external magnet is placed nearby.MicroRNA-128 (miR-128) is an attractive therapeutic molecule with powerful glioblastoma regulation properties. However, miR-128 lacks biological stability and leads to poor delivery efficacy in clinical applications. In our previous study, we demonstrated two effective transgene carriers, including polyethylenimine (PEI)-decorated superparamagnetic iron oxide nanoparticles (SPIONs) as well as chemically-conjugated chondroitin sulfate-PEI copolymers (CPs). In this contribution, we report optimized conditions for coating CPs onto the surfaces of SPIONs, forming CPIOs, for magneto-gene delivery systems. The optimized weight ratio of the CPs and SPIONs is 2 : 1, which resulted in the formation of a stable particle as a good transgene carrier. The hydrodynamic diameter of the CPIOs is ~136 nm. The gel electrophoresis results demonstrate that the weight ratio of CPIO/DNA required to completely encapsulate pDNA is >=3. The in vitro tests of CPIO/DNA were done in 293 T, CRL5802, and U87-MG cells in the presence and absence of an external magnetic field. The magnetofection efficiency of CPIO/DNA was measured in the three cell lines with or without fetal bovine serum (FBS). CPIO/DNA exhibited remarkably improved gene expression in the presence of the magnetic field and 10% FBS as compared with a gold non-viral standard, PEI/DNA, and a commercial magnetofection reagent, PolyMag/DNA. In addition, CPIO/DNA showed less cytotoxicity than PEI/DNA and PolyMag/DNA against the three cell lines. The transfection efficiency of the magnetoplex improved significantly with an assisted magnetic field. In miR-128 delivery, a microRNA plate array and fluorescence in situ hybridization were used to demonstrate that CPIO/pMIRNA-128 indeed expresses more miR-128 with the assisted magnetic field than without. In a biodistribution test, CPIO/Cy5-DNA showed higher accumulation at the tumor site where an external magnet is placed nearby. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr01404b

DNA-based species level detection of Glomeromycota: one PCR primer set for all arbuscular mycorrhizal fungi.

PubMed

Krüger, Manuela; Stockinger, Herbert; Krüger, Claudia; Schüssler, Arthur

2009-01-01

* At present, molecular ecological studies of arbuscular mycorrhizal fungi (AMF) are only possible above species level when targeting entire communities. To improve molecular species characterization and to allow species level community analyses in the field, a set of newly designed AMF specific PCR primers was successfully tested. * Nuclear rDNA fragments from diverse phylogenetic AMF lineages were sequenced and analysed to design four primer mixtures, each targeting one binding site in the small subunit (SSU) or large subunit (LSU) rDNA. To allow species resolution, they span a fragment covering the partial SSU, whole internal transcribed spacer (ITS) rDNA region and partial LSU. * The new primers are suitable for specifically amplifying AMF rDNA from material that may be contaminated by other organisms (e.g., samples from pot cultures or the field), characterizing the diversity of AMF species from field samples, and amplifying a SSU-ITS-LSU fragment that allows phylogenetic analyses with species level resolution. * The PCR primers can be used to monitor entire AMF field communities, based on a single rDNA marker region. Their application will improve the base for deep sequencing approaches; moreover, they can be efficiently used as DNA barcoding primers.

Frequency and Voltage Dependence of the Dielectrophoretic Trapping of Short Lengths of DNA and dCTP in a Nanopipette

PubMed Central

Ying, Liming; White, Samuel S.; Bruckbauer, Andreas; Meadows, Lisa; Korchev, Yuri E.; Klenerman, David

2004-01-01

The study of the properties of DNA under high electric fields is of both fundamental and practical interest. We have exploited the high electric fields produced locally in the tip of a nanopipette to probe the motion of double- and single-stranded 40-mer DNA, a 1-kb single-stranded DNA, and a single-nucleotide triphosphate (dCTP) just inside and outside the pipette tip at different frequencies and amplitudes of applied voltages. We used dual laser excitation and dual color detection to simultaneously follow two fluorophore-labeled DNA sequences with millisecond time resolution, significantly faster than studies to date. A strong trapping effect was observed during the negative half cycle for all DNA samples and also the dCTP. This effect was maximum below 1 Hz and decreased with higher frequency. We assign this trapping to strong dielectrophoresis due to the high electric field and electric field gradient in the pipette tip. Dielectrophoresis in electrodeless tapered nanostructures has potential applications for controlled mixing and manipulation of short lengths of DNA and other biomolecules, opening new possibilities in miniaturized biological analysis. PMID:14747337

Microwave-field-driven acoustic modes in DNA.

PubMed Central

Edwards, G S; Davis, C C; Saffer, J D; Swicord, M L

1985-01-01

The direct coupling of a microwave field to selected DNA molecules is demonstrated using standard dielectrometry. The absorption is resonant with a typical lifetime of 300 ps. Such a long lifetime is unexpected for DNA in aqueous solution at room temperature. Resonant absorption at fundamental and harmonic frequencies for both supercoiled circular and linear DNA agrees with an acoustic mode model. Our associated acoustic velocities for linear DNA are very close to the acoustic velocity of the longitudinal acoustic mode independently observed on DNA fibers using Brillouin spectroscopy. The difference in acoustic velocities for supercoiled circular and linear DNA is discussed in terms of solvent shielding of the nonbonded potentials in DNA. Images FIGURE 5 FIGURE 6 FIGURE 7 PMID:3893557

Profiling Nematode Communities in Unmanaged Flowerbed and Agricultural Field Soils in Japan by DNA Barcode Sequencing

PubMed Central

Morise, Hisashi; Miyazaki, Erika; Yoshimitsu, Shoko; Eki, Toshihiko

2012-01-01

Soil nematodes play crucial roles in the soil food web and are a suitable indicator for assessing soil environments and ecosystems. Previous nematode community analyses based on nematode morphology classification have been shown to be useful for assessing various soil environments. Here we have conducted DNA barcode analysis for soil nematode community analyses in Japanese soils. We isolated nematodes from two different environmental soils of an unmanaged flowerbed and an agricultural field using the improved flotation-sieving method. Small subunit (SSU) rDNA fragments were directly amplified from each of 68 (flowerbed samples) and 48 (field samples) isolated nematodes to determine the nucleotide sequence. Sixteen and thirteen operational taxonomic units (OTUs) were obtained by multiple sequence alignment from the flowerbed and agricultural field nematodes, respectively. All 29 SSU rDNA-derived OTUs (rOTUs) were further mapped onto a phylogenetic tree with 107 known nematode species. Interestingly, the two nematode communities examined were clearly distinct from each other in terms of trophic groups: Animal predators and plant feeders were markedly abundant in the flowerbed soils, in contrast, bacterial feeders were dominantly observed in the agricultural field soils. The data from the flowerbed nematodes suggests a possible food web among two different trophic nematode groups and plants (weeds) in the closed soil environment. Finally, DNA sequences derived from the mitochondrial cytochrome oxidase c subunit 1 (COI) gene were determined as a DNA barcode from 43 agricultural field soil nematodes. These nematodes were assigned to 13 rDNA-derived OTUs, but in the COI gene analysis were assigned to 23 COI gene-derived OTUs (cOTUs), indicating that COI gene-based barcoding may provide higher taxonomic resolution than conventional SSU rDNA-barcoding in soil nematode community analysis. PMID:23284767

Field calibration of blowfly-derived DNA against traditional methods for assessing mammal diversity in tropical forests.

PubMed

Lee, Ping-Shin; Gan, Han Ming; Clements, Gopalasamy Reuben; Wilson, John-James

2016-11-01

Mammal diversity assessments based on DNA derived from invertebrates have been suggested as alternatives to assessments based on traditional methods; however, no study has field-tested both approaches simultaneously. In Peninsular Malaysia, we calibrated the performance of mammal DNA derived from blowflies (Diptera: Calliphoridae) against traditional methods used to detect species. We first compared five methods (cage trapping, mist netting, hair trapping, scat collection, and blowfly-derived DNA) in a forest reserve with no recent reports of megafauna. Blowfly-derived DNA and mist netting detected the joint highest number of species (n = 6). Only one species was detected by multiple methods. Compared to the other methods, blowfly-derived DNA detected both volant and non-volant species. In another forest reserve, rich in megafauna, we calibrated blowfly-derived DNA against camera traps. Blowfly-derived DNA detected more species (n = 11) than camera traps (n = 9), with only one species detected by both methods. The rarefaction curve indicated that blowfly-derived DNA would continue to detect more species with greater sampling effort. With further calibration, blowfly-derived DNA may join the list of traditional field methods. Areas for further investigation include blowfly feeding and dispersal biology, primer biases, and the assembly of a comprehensive and taxonomically-consistent DNA barcode reference library.

A simplified field protocol for genetic sampling of birds using buccal swabs

USGS Publications Warehouse

Vilstrup, Julia T.; Mullins, Thomas D.; Miller, Mark P.; McDearman, Will; Walters, Jeffrey R.; Haig, Susan M.

2018-01-01

DNA sampling is an essential prerequisite for conducting population genetic studies. For many years, blood sampling has been the preferred method for obtaining DNA in birds because of their nucleated red blood cells. Nonetheless, use of buccal swabs has been gaining favor because they are less invasive yet still yield adequate amounts of DNA for amplifying mitochondrial and nuclear markers; however, buccal swab protocols often include steps (e.g., extended air-drying and storage under frozen conditions) not easily adapted to field settings. Furthermore, commercial extraction kits and swabs for buccal sampling can be expensive for large population studies. We therefore developed an efficient, cost-effective, and field-friendly protocol for sampling wild birds after comparing DNA yield among 3 inexpensive buccal swab types (2 with foam tips and 1 with a cotton tip). Extraction and amplification success was high (100% and 97.2% respectively) using inexpensive generic swabs. We found foam-tipped swabs provided higher DNA yields than cotton-tipped swabs. We further determined that omitting a drying step and storing swabs in Longmire buffer increased efficiency in the field while still yielding sufficient amounts of DNA for detailed population genetic studies using mitochondrial and nuclear markers. This new field protocol allows time- and cost-effective DNA sampling of juveniles or small-bodied birds for which drawing blood may cause excessive stress to birds and technicians alike.

Structural DNA Nanotechnology: Artificial Nanostructures for Biomedical Research.

PubMed

Ke, Yonggang; Castro, Carlos; Choi, Jong Hyun

2018-06-04

Structural DNA nanotechnology utilizes synthetic or biologic DNA as designer molecules for the self-assembly of artificial nanostructures. The field is founded upon the specific interactions between DNA molecules, known as Watson-Crick base pairing. After decades of active pursuit, DNA has demonstrated unprecedented versatility in constructing artificial nanostructures with significant complexity and programmability. The nanostructures could be either static, with well-controlled physicochemical properties, or dynamic, with the ability to reconfigure upon external stimuli. Researchers have devoted considerable effort to exploring the usability of DNA nanostructures in biomedical research. We review the basic design methods for fabricating both static and dynamic DNA nanostructures, along with their biomedical applications in fields such as biosensing, bioimaging, and drug delivery.

Triple Track: A New Paradigm for Developing Air Force Officers

DTIC Science & Technology

2017-03-06

multiple career fields, including aircraft maintenance, command and control, engineering , modeling analysis, joint targeting, program management...the opportunity for an engineering job because he was too critical in his career field. The member ultimately left the service.41 Critical manning...manpower, and officer training. Over the course of her career , she was responsible for generating over 956 C-130 aircraft sorties, led interagency efforts

Naval Biodynamics Laboratory 1993 Command History

DTIC Science & Technology

1993-01-01

position and alignment, camera optical calibration, photo target position, and standard anatomical coordinate systems based upon X-rays of each HRV...safety range. Before, during, and after each sled run, a physiological data acquisition system is used to collect and analyze physiological measurements ...experimental devices. It is also responsible for the configuring of field data measuring and acquisition systems for use aboard ships or at other field

10th Annual Small Business Conference

DTIC Science & Technology

2006-11-02

has extensive process knowledge in military, space and commercial. Steve worked many corporate initiatives, and led a leveraging group that made a...2001, Mr. Loehrl served as Chief of the Chemical Demilitarization program, a $ 12 billion mission that was new to the Command. His - 1 - - 1... Trivia Question Apple Trivia Question Focus Right Product Right Time Right Place Right Price Rapid Fielding Initiative Equipment Items Fielded to All

Photocopy of drawing (original drawing of MacDill Field in possession ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of MacDill Field in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1952 architectural drawings by Strategic Air Command, MacDill Air Force Base) BASE LAYOUT, 1952 - MacDill Air Force Base, Bounded by City of Tampa North, Tampa Bay South, Old Tampa Bay West, & Hillsborough Bay East, Tampa, Hillsborough County, FL

STS-104 Crew Return, Ellington Field, Building 990

NASA Image and Video Library

2001-07-26

JSC2001-E-22791 (25 July 2001) --- JSC Acting Director Roy S. Estess introduces the STS-104 crew members to a crowd gathered in Ellington Field’s Hangar 990 during crew return ceremonies. Seated (from left) are Michael L. Gernhardt, James F. Reilly, Janet L. Kavandi, all mission specialists, along with Steven W. Lindsey and Charles O. Hobaugh, mission commander and pilot, respectively.

Autonomous Command Operations of the WIRE Spacecraft

NASA Technical Reports Server (NTRS)

Walyus, Keith; Prior, Mike; Saylor, Richard

1999-01-01

This paper presents operational innovations which will be introduced on NASA's Wide Field Infrared Explorer (WIRE) mission. These innovations include an end-to-end design architecture for an autonomous commanding capability for the uplink of command loads during unattended station contacts. The WIRE mission is the fifth and final mission of NASA's Goddard Space Flight Center Small Explorer (SMEX) series to be launched in March of 1999. Its primary mission is the targeting of deep space fields using an ultra-cooled infrared telescope. Due to its mission design WIRE command loads are large (approximately 40 Kbytes per 24 hours) and must be performed daily. To reduce the cost of mission operations support that would be required in order to uplink command loads, the WIRE Flight Operations Team has implemented all autonomous command loading capability. This capability allows completely unattended operations over a typical two-day weekend period. The key factors driving design and implementation of this capability were: 1) integration with already existing ground system autonomous capabilities and systems, 2) the desire to evolve autonomous operations capabilities based upon previous SMEX operations experience - specifically the TRACE mission, 3) integration with ground station operations - both autonomous and man-tended, 4) low cost and quick implementation, and 5) end-to-end system robustness. A trade-off study was performed to examine these factors in light of the low-cost, higher-risk SMEX mission philosophy. The study concluded that a STOL (Spacecraft Test and Operations Language) based script, highly integrated with other scripts used to perform autonomous operations, was best suited given the budget and goals of the mission. Each of these factors is discussed in addition to use of the TRACE mission as a testbed for autonomous commanding prior to implementation on WIRE. The capabilities implemented on the WIRE mission are an example of a low-cost, robust, and efficient method for autonomous command loading when implemented with other autonomous features of the ground system. They call be used as a design and implementation template by other missions interested in evolving toward autonomous and lower cost operations. Additionally, the WIRE spacecraft will be used as an operational testbed upon completion of its nominal mission later in 1999. One idea being studied is advanced on-board modeling. Advanced on-board modeling techniques will be used to more efficiently display the spacecraft state. This health and safety information could be used by engineers on the ground or could be used by tile spacecraft for its own assessments. Additionally, this same state information could also be input into the event-driven scheduling system, as the scheduling system will need to assess the spacecraft state before undertaking a new activity. Advanced modeling techniques are being evaluated for a number of NASA missions including The Next Generation Space Telescope (NGST), which is scheduled to launch in 2007.

Characteristics and oxidative stress on rats and traffic policemen of ambient fine particulate matter from Shenyang.

PubMed

Ma, Mingyue; Li, Shuyin; Jin, Huanrong; Zhang, Yumin; Xu, Jia; Chen, Dongmei; Kuimin, Chen; Yuan, Zhou; Xiao, Chunling

2015-09-01

Fine particulate matter (PM2.5) pollution is becoming serious in China. This study aimed to investigate the impact of PM2.5 on DNA damage in Shenyang city. The concentration and composition of PM2.5 in traffic policemen's working sites including fields and indoor offices were obtained. Blood samples of field and office policemen were collected to detect DNA damage by Comet assay. Rats were used to further analyzing the oxidative DNA damage. The average concentration of PM2.5 in exposed group was significantly higher than that in control group. Composition analysis revealed that toxic heavy metal and polycyclic aromatic hydrocarbon substances were main elements of this PM2.5. DNA damage in field policemen was significantly higher than those in non-field group. Moreover, animal studies confirmed the oxidative DNA damage induced by PM2.5. Taken together, high DNA damages are found in the Shenyang traffic policemen and rats exposed to high level of airborne PM2.5. Copyright © 2015 Elsevier B.V. All rights reserved.

Tilted hexagonal post arrays: DNA electrophoresis in anisotropic media

PubMed Central

Chen, Zhen; Dorfman, Kevin D.

2013-01-01

Using Brownian dynamics simulations, we show that DNA electrophoresis in a hexagonal array of micron-sized posts changes qualitatively when the applied electric field vector is not coincident with the lattice vectors of the array. DNA electrophoresis in such “tilted” post arrays is superior to the standard “un-tilted” approach; while the time required to achieve a resolution of unity in a tilted post array is similar to an un-tilted array at a low electric field strengths, this time (i) decreases exponentially with electric field strength in a tilted array and (ii) increases exponentially with electric field strength in an un-tilted array. Although the DNA dynamics in a post array are complicated, the electrophoretic mobility results indicate that the “free path”, i.e., the average distance of ballistic trajectories of point sized particles launched from random positions in the unit cell until they intersect the next post, is a useful proxy for the detailed DNA trajectories. The analysis of the free path reveals a fundamental connection between anisotropy of the medium and DNA transport therein that goes beyond simply improving the separation device. PMID:23868490

[Genetic and epigenetic news in gerontology].

PubMed

Baranov, V S; Glotov, O S; Baranova, E V

2014-01-01

The overview represents the recent most conspicuous findings in aging studies. It includes new data on the whole genome association studies (GWAS) in big cohort of centenaries, recently found mutation protecting from Alzheimer disease, discovery of hypothalamus as a command center of human aging, very important data on the negative effect of common antioxidants in the treatment of lung cancer as well as new data concerning antiaging and anticancer effects of common drugs such as rapamycine and metformin. Substantial part of the review is devoted to the epigenetic problems of senescence and feasible impact of basic epigenetic mechanisms (methylation of DNA and histone proteins, DNA heterochromatization) in regulation of gene expression, long-term genome reprogramming during early childhood, and transgeneration transmission of epigenetic traits. The necessity of transition from molecular studies of dormant human genome (anatomy of human genome) to genome in action (dynamic genome) and thus with special emphasis to epigenetic medicine is stressed.

Architecture with GIDEON, A Program for Design in Structural DNA Nanotechnology

PubMed Central

Birac, Jeffrey J.; Sherman, William B.; Kopatsch, Jens; Constantinou, Pamela E.; Seeman, Nadrian C.

2012-01-01

We present geometry based design strategies for DNA nanostructures. The strategies have been implemented with GIDEON – a Graphical Integrated Development Environment for OligoNucleotides. GIDEON has a highly flexible graphical user interface that facilitates the development of simple yet precise models, and the evaluation of strains therein. Models are built on a simple model of undistorted B-DNA double-helical domains. Simple point and click manipulations of the model allow the minimization of strain in the phosphate-backbone linkages between these domains and the identification of any steric clashes that might occur as a result. Detailed analysis of 3D triangles yields clear predictions of the strains associated with triangles of different sizes. We have carried out experiments that confirm that 3D triangles form well only when their geometrical strain is less than 4% deviation from the estimated relaxed structure. Thus geometry-based techniques alone, without energetic considerations, can be used to explain general trends in DNA structure formation. We have used GIDEON to build detailed models of double crossover and triple crossover molecules, evaluating the non-planarity associated with base tilt and junction mis-alignments. Computer modeling using a graphical user interface overcomes the limited precision of physical models for larger systems, and the limited interaction rate associated with earlier, command-line driven software. PMID:16630733

Molecular dynamics simulations of polarizable DNA in crystal environment

NASA Astrophysics Data System (ADS)

Babin, Volodymyr; Baucom, Jason; Darden, Thomas A.; Sagui, Celeste

We have investigated the role of the electrostatic description and cell environment in molecular dynamics (MD) simulations of DNA. Multiple unrestrained MD simulations of the DNA duplex d(CCAACGTTGG)2 have been carried out using two different force fields: a traditional description based on atomic point charges and a polarizable force field. For the time scales probed, and given the ?right? distribution of divalent ions, the latter performs better than the nonpolarizable force field. In particular, by imposing the experimental unit cell environment, an initial configuration with ideal B-DNA duplexes in the unit cell acquires sequence-dependent features that very closely resemble the crystallographic ones. Simultaneously, the all-atom root-mean-square coordinates deviation (RMSD) with respect to the crystallographic structure is seen to decay. At later times, the polarizable force field is able to maintain this lower RMSD, while the nonpolarizable force field starts to drift away.

Field Artillery and the Combined Arms Team: A Case for the Continued Relevance of American Fire Support

DTIC Science & Technology

2015-05-23

integrate with maneuver forces to meet future threats. Several former brigade combat team commanders mentioned the field artillery’s “identity crisis ” in...September 2008): 35. 5 Sean MacFarland, Michael Shields, and Jeffrey Snow, “White Paper: The King and I: The Impeding Crisis in Field Artillery’s Ability...Michael S. Coombes , “Agile Fires and Decisive Action: Achieving Pervasive Agility by Focusing On Fundamentals,” NTC Decisive Action Training

Leadership -- Analysis and Comment

DTIC Science & Technology

1991-02-18

perfection: rather, put in wnat needs r; ~e por in to get the organizacIon moving. The return on pertecv~or s extra effort Is going to oe far more expensive...Department of the Army, Field Manual 25-100. Traininq The Force, pp. 1-5. 4. U.S. Department of the Army, Field Manual 22-103. Leadership and Commana at Senior...4. U.S. Department of the Army, Field Manual 22-103, Leadership and Command at Senior Levels, pp. 60-66. 5. Bennis, p. 22. 6. Michael P. W. Stone

GEOTHERM user guide

USGS Publications Warehouse

Swanson, James R.

1977-01-01

GEOTHERM is a computerized geothermal resources file developed by the U.S. Geological Survey. The file contains data on geothermal fields, wells, and chemical analyses from the United states and international sources. The General Information Processing System (GIPSY) in the IBM 370/155 computer is used to store and retrieve data. The GIPSY retrieval program contains simple commands which can be used to search the file, select a narrowly defined subset, sort the records, and output the data in a variety of forms. Eight commands are listed and explained so that the GEOTHERM file can be accessed directly by geologists. No programming experience is necessary to retrieve data from the file.

DNA curvature and flexibility in vitro and in vivo

PubMed Central

Peters, Justin P.; Maher, L. James

2014-01-01

It has been more than 50 years since the elucidation of the structure of double-helical DNA. Despite active research and progress in DNA biology and biochemistry, much remains to be learned in the field of DNA biophysics. Predicting the sequence-dependent curvature and flexibility of DNA is difficult. Applicability of the conventional worm-like chain polymer model of DNA has been challenged. The fundamental forces responsible for the remarkable resistance of DNA to bending and twisting remain controversial. The apparent “softening” of DNA measured in vivo in the presence of kinking proteins and superhelical strain is incompletely understood. New methods and insights are being applied to these problems. This review places current work on DNA biophysics in historical context and illustrates the ongoing interplay between theory and experiment in this exciting field. PMID:20478077

32 CFR 158.3 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-07-01

... routinely are put in place to provide support to many newly fielded weapons systems, including aircraft, land combat vehicles, and automated command and control systems. Systems support contracting authority... generally has less control over systems support contracts than other types of contracts. Theater business...

32 CFR 158.3 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... routinely are put in place to provide support to many newly fielded weapons systems, including aircraft, land combat vehicles, and automated command and control systems. Systems support contracting authority... generally has less control over systems support contracts than other types of contracts. Theater business...

32 CFR 158.3 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... routinely are put in place to provide support to many newly fielded weapons systems, including aircraft, land combat vehicles, and automated command and control systems. Systems support contracting authority... generally has less control over systems support contracts than other types of contracts. Theater business...

Simulations Meet Experiment to Reveal New Insights into DNA Intrinsic Mechanics

PubMed Central

Ben Imeddourene, Akli; Elbahnsi, Ahmad; Guéroult, Marc; Oguey, Christophe; Foloppe, Nicolas; Hartmann, Brigitte

2015-01-01

The accurate prediction of the structure and dynamics of DNA remains a major challenge in computational biology due to the dearth of precise experimental information on DNA free in solution and limitations in the DNA force-fields underpinning the simulations. A new generation of force-fields has been developed to better represent the sequence-dependent B-DNA intrinsic mechanics, in particular with respect to the BI ↔ BII backbone equilibrium, which is essential to understand the B-DNA properties. Here, the performance of MD simulations with the newly updated force-fields Parmbsc0εζOLI and CHARMM36 was tested against a large ensemble of recent NMR data collected on four DNA dodecamers involved in nucleosome positioning. We find impressive progress towards a coherent, realistic representation of B-DNA in solution, despite residual shortcomings. This improved representation allows new and deeper interpretation of the experimental observables, including regarding the behavior of facing phosphate groups in complementary dinucleotides, and their modulation by the sequence. It also provides the opportunity to extensively revisit and refine the coupling between backbone states and inter base pair parameters, which emerges as a common theme across all the complementary dinucleotides. In sum, the global agreement between simulations and experiment reveals new aspects of intrinsic DNA mechanics, a key component of DNA-protein recognition. PMID:26657165

Using occupancy modelling to compare environmental DNA to traditional field methods for regional-scale monitoring of an endangered aquatic species.

PubMed

Schmelzle, Molly C; Kinziger, Andrew P

2016-07-01

Environmental DNA (eDNA) monitoring approaches promise to greatly improve detection of rare, endangered and invasive species in comparison with traditional field approaches. Herein, eDNA approaches and traditional seining methods were applied at 29 research locations to compare method-specific estimates of detection and occupancy probabilities for endangered tidewater goby (Eucyclogobius newberryi). At each location, multiple paired seine hauls and water samples for eDNA analysis were taken, ranging from two to 23 samples per site, depending upon habitat size. Analysis using a multimethod occupancy modelling framework indicated that the probability of detection using eDNA was nearly double (0.74) the rate of detection for seining (0.39). The higher detection rates afforded by eDNA allowed determination of tidewater goby occupancy at two locations where they have not been previously detected and at one location considered to be locally extirpated. Additionally, eDNA concentration was positively related to tidewater goby catch per unit effort, suggesting eDNA could potentially be used as a proxy for local tidewater goby abundance. Compared to traditional field sampling, eDNA provided improved occupancy parameter estimates and can be applied to increase management efficiency across a broad spatial range and within a diversity of habitats. © 2015 John Wiley & Sons Ltd.

Genotoxicity in native fish associated with agricultural runoff events

USGS Publications Warehouse

Whitehead, Andrew; Kuivila, Kathryn; Orlando, James L.; Kotelevtsev, S.; Anderson, Susan L.

2004-01-01

The primary objective of the present study was to test whether agricultural chemical runoff was associated with in-stream genotoxicity in native fish. Using Sacramento sucker (Catostomus occidentalis), we combined field-caging experiments in an agriculturally dominated watershed with controlled laboratory exposures to field-collected water samples, and we coupled genotoxicity biomarker measurements in fish with bacterial mutagenicity analysis of water samples. We selected DNA strand breakage as a genotoxicity biomarker and Ames Salmonella mutagenicity tests as a second, supporting indicator of genotoxicity. Data from experiments conducted during rainfall runoff events following winter application of pesticides in 2000 and 2001 indicated that DNA strand breaks were significantly elevated in fish exposed to San Joaquin River (CA, USA) water (38.8, 28.4, and 53.6% DNA strand breakage in year 2000 field, year 2000 lab, and year 2001 field exposures, respectively) compared with a nearby reference site (15.4, 8.7, and 12.6% DNA strand breakage in year 2000 field, year 2000 lab, and year 2001 field exposures, respectively). Time-course measurements in field experiments supported a linkage between induction of DNA strand breakage and the timing of agricultural runoff. San Joaquin River water also caused significant reversion mutation in two Ames Salmonella tester strains. Salmonella mutagenicity corroborated in-stream effects, further strengthening a causal relationship between runoff events and genotoxicity. Potentially responsible agents are discussed in the context of timing of runoff events in the field, concordance between laboratory and field exposures, pesticide application patterns in the drainage, and analytical chemistry data.

Cognitive Systems Modeling and Analysis of Command and Control Systems

NASA Technical Reports Server (NTRS)

Norlander, Arne

2012-01-01

Military operations, counter-terrorism operations and emergency response often oblige operators and commanders to operate within distributed organizations and systems for safe and effective mission accomplishment. Tactical commanders and operators frequently encounter violent threats and critical demands on cognitive capacity and reaction time. In the future they will make decisions in situations where operational and system characteristics are highly dynamic and non-linear, i.e. minor events, decisions or actions may have serious and irreversible consequences for the entire mission. Commanders and other decision makers must manage true real time properties at all levels; individual operators, stand-alone technical systems, higher-order integrated human-machine systems and joint operations forces alike. Coping with these conditions in performance assessment, system development and operational testing is a challenge for both practitioners and researchers. This paper reports on research from which the results led to a breakthrough: An integrated approach to information-centered systems analysis to support future command and control systems research development. This approach integrates several areas of research into a coherent framework, Action Control Theory (ACT). It comprises measurement techniques and methodological advances that facilitate a more accurate and deeper understanding of the operational environment, its agents, actors and effectors, generating new and updated models. This in turn generates theoretical advances. Some good examples of successful approaches are found in the research areas of cognitive systems engineering, systems theory, and psychophysiology, and in the fields of dynamic, distributed decision making and naturalistic decision making.

Technological evaluation of gesture and speech interfaces for enabling dismounted soldier-robot dialogue

NASA Astrophysics Data System (ADS)

Kattoju, Ravi Kiran; Barber, Daniel J.; Abich, Julian; Harris, Jonathan

2016-05-01

With increasing necessity for intuitive Soldier-robot communication in military operations and advancements in interactive technologies, autonomous robots have transitioned from assistance tools to functional and operational teammates able to service an array of military operations. Despite improvements in gesture and speech recognition technologies, their effectiveness in supporting Soldier-robot communication is still uncertain. The purpose of the present study was to evaluate the performance of gesture and speech interface technologies to facilitate Soldier-robot communication during a spatial-navigation task with an autonomous robot. Gesture and speech semantically based spatial-navigation commands leveraged existing lexicons for visual and verbal communication from the U.S Army field manual for visual signaling and a previously established Squad Level Vocabulary (SLV). Speech commands were recorded by a Lapel microphone and Microsoft Kinect, and classified by commercial off-the-shelf automatic speech recognition (ASR) software. Visual signals were captured and classified using a custom wireless gesture glove and software. Participants in the experiment commanded a robot to complete a simulated ISR mission in a scaled down urban scenario by delivering a sequence of gesture and speech commands, both individually and simultaneously, to the robot. Performance and reliability of gesture and speech hardware interfaces and recognition tools were analyzed and reported. Analysis of experimental results demonstrated the employed gesture technology has significant potential for enabling bidirectional Soldier-robot team dialogue based on the high classification accuracy and minimal training required to perform gesture commands.

Single DNA imaging and length quantification through a mobile phone microscope

NASA Astrophysics Data System (ADS)

Wei, Qingshan; Luo, Wei; Chiang, Samuel; Kappel, Tara; Mejia, Crystal; Tseng, Derek; Chan, Raymond Yan L.; Yan, Eddie; Qi, Hangfei; Shabbir, Faizan; Ozkan, Haydar; Feng, Steve; Ozcan, Aydogan

2016-03-01

The development of sensitive optical microscopy methods for the detection of single DNA molecules has become an active research area which cultivates various promising applications including point-of-care (POC) genetic testing and diagnostics. Direct visualization of individual DNA molecules usually relies on sophisticated optical microscopes that are mostly available in well-equipped laboratories. For POC DNA testing/detection, there is an increasing need for the development of new single DNA imaging and sensing methods that are field-portable, cost-effective, and accessible for diagnostic applications in resource-limited or field-settings. For this aim, we developed a mobile-phone integrated fluorescence microscopy platform that allows imaging and sizing of single DNA molecules that are stretched on a chip. This handheld device contains an opto-mechanical attachment integrated onto a smartphone camera module, which creates a high signal-to-noise ratio dark-field imaging condition by using an oblique illumination/excitation configuration. Using this device, we demonstrated imaging of individual linearly stretched λ DNA molecules (48 kilobase-pair, kbp) over 2 mm2 field-of-view. We further developed a robust computational algorithm and a smartphone app that allowed the users to quickly quantify the length of each DNA fragment imaged using this mobile interface. The cellphone based device was tested by five different DNA samples (5, 10, 20, 40, and 48 kbp), and a sizing accuracy of <1 kbp was demonstrated for DNA strands longer than 10 kbp. This mobile DNA imaging and sizing platform can be very useful for various diagnostic applications including the detection of disease-specific genes and quantification of copy-number-variations at POC settings.

Preservation of RNA and DNA from mammal samples under field conditions.

PubMed

Camacho-Sanchez, Miguel; Burraco, Pablo; Gomez-Mestre, Ivan; Leonard, Jennifer A

2013-07-01

Ecological and conservation genetics require sampling of organisms in the wild. Appropriate preservation of the collected samples, usually by cryostorage, is key to the quality of the genetic data obtained. Nevertheless, cryopreservation in the field to ensure RNA and DNA stability is not always possible. We compared several nucleic acid preservation solutions appropriate for field sampling and tested them on rat (Rattus rattus) blood, ear and tail tip, liver, brain and muscle. We compared the efficacy of a nucleic acid preservation (NAP) buffer for DNA preservation against 95% ethanol and Longmire buffer, and for RNA preservation against RNAlater (Qiagen) and Longmire buffer, under simulated field conditions. For DNA, the NAP buffer was slightly better than cryopreservation or 95% ethanol, but high molecular weight DNA was preserved in all conditions. The NAP buffer preserved RNA as well as RNAlater. Liver yielded the best RNA and DNA quantity and quality; thus, liver should be the tissue preferentially collected from euthanized animals. We also show that DNA persists in nonpreserved muscle tissue for at least 1 week at ambient temperature, although degradation is noticeable in a matter of hours. When cryopreservation is not possible, the NAP buffer is an economical alternative for RNA preservation at ambient temperature for at least 2 months and DNA preservation for at least 10 months. © 2013 John Wiley & Sons Ltd.

High-throughput DNA separation in nanofilter arrays.

PubMed

Choi, Sungup; Kim, Ju Min; Ahn, Kyung Hyun; Lee, Seung Jong

2014-08-01

We numerically investigated the dynamics of short double-stranded DNA molecules moving through a deep-shallow alternating nanofilter, by utilizing Brownian dynamics simulation. We propose a novel mechanism for high-throughput DNA separation with a high electric field, which was originally predicted by Laachi et al. [Phys. Rev. Lett. 2007, 98, 098106]. In this work, we show that DNA molecules deterministically move along different electrophoretic streamlines according to their length, owing to geometric constraint at the exit of the shallow region. Consequently, it is more probable that long DNA molecules pass over a deep well region without significant lateral migration toward the bottom of the deep well, which is in contrast to the long dwelling time for short DNA molecules. We investigated the dynamics of DNA passage through a nanofilter facilitating electrophoretic field kinematics. The statistical distribution of the DNA molecules according to their size clearly corroborates our assumption. On the other hand, it was also found that the tapering angle between the shallow and deep regions significantly affects the DNA separation performance. The current results show that the nonuniform field effect combined with geometric constraint plays a key role in nanofilter-based DNA separation. We expect that our results will be helpful in designing and operating nanofluidics-based DNA separation devices and in understanding the polymer dynamics in confined geometries. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A Programmable and Configurable Mixed-Mode FPAA SoC

DTIC Science & Technology

2016-03-17

A Programmable and Configurable Mixed-Mode FPAA SoC Sahil Shah, Sihwan Kim, Farhan Adil, Jennifer Hasler, Suma George, Michelle Collins, Richard...Abstract: The authors present a Floating-Gate based, System-On-Chip large-scale Field- Programmable Analog Array IC that integrates divergent concepts...Floating-Gate, SoC, Command Word Classification This paper presents a Floating-Gate (FG) based, System- On-Chip (SoC) large-scale Field- Programmable

Depth vs. Breadth: Talent Management for Special Warfare

DTIC Science & Technology

2016-12-01

discuss the relevant aspects of how competitive sports teams think about and manage the inevitable churn of their most talented players ; I will...leadership—the research determines that the current method of rotating field grade officers through key jobs every 12–24 months is antithetical to the...interviews with former four- star commanders and Defense Department leadership—the research determines that the current method of rotating field grade

Effects of Command and Control Vehicle (C2V) Operational Environment on Soldier Health and Performance

NASA Technical Reports Server (NTRS)

Cowings, Patricia S.; Toscano, William B.; DeRoshia, Charles; Tauson, Richard

1999-01-01

The purpose of this project was to use NASA technology to assist the US Army in the assessment of motion sickness and performance of soldiers in the Command and Control Vehicle (C2V). Three different vehicle configurations were tested: oblique, (3 seats at a 20-degree angle from the direction of travel); perpendicular, (3 seats at a 90 degree angle); and 4-forward, (all seats faced forward). In all vehicles, the front seat faced forward. Sixteen men and eight women participated for 15 days: 2 days of classroom instruction; 12 days of field tests in the C2V, and 15 minutes of post-field test performance measures. Conditions for field tests were: an initial Park; four Moves (i.e., travel over a mixed terrain); and four Short-halts following movement. NASA task batteries, mood and symptom scales, and physiological data were collected during field tests. Motion sickness symptoms ranging from slight to severe were reported for all subjects. Conclusions were: (1) there was no difference between vehicle configurations; (2) there was a negative impact on crew performance and health when subjects attended to visual screens during vehicle movement; and (3) symptoms and performance degradation were not mitigated by intermittent short-halts.

STS-130 Nationals Park Visit

NASA Image and Video Library

2010-04-20

NASA STS-130 crew Commander George Zamka, far left, Pilot Terry Virts, Mission Specialists Kathryn Hire, Stephen Robinson, Nicholas Patrick and Robert Behnken, far right, pose for pictures on the field at Nationals Park, Tuesday, April 20, 2010, in Washington. Photo Credit: (NASA/Carla Cioffi)

32 CFR 651.6 - NEPA analysis staffing.

Code of Federal Regulations, 2010 CFR

2010-07-01

... National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) ENVIRONMENTAL QUALITY... management is not in the proponents' chain of command (for example, installation management of a range for MATDEV testing or installation management of a fielding location), the proponent shall coordinate the...

STS-99 crewmembers in aircraft prior to leaving Ellington Field for KSC

NASA Image and Video Library

2000-01-27

JSC2000-00924 (27 January 2000) --- Astronaut Kevin R. Kregel, mission commander, signals thumbs up as he prepares to pilot a T-38 jet aircraft to southern Florida in preparation for launch next week aboard the Space Shuttle Endeavour.

The Rigors of Aligning Performance

DTIC Science & Technology

2015-06-01

organization merged 6 its field activities into regional facilities engineering commands (FECs). Today, FECs provide one-stop shopping for NAVFAC clients...many are old and antiquated , sometimes the systems mesh together other times they do not. Training is lacking on the various systems.  Communication

Detection of influenza A virus using carbon nanotubes field effect transistor based DNA sensor

NASA Astrophysics Data System (ADS)

Tran, Thi Luyen; Nguyen, Thi Thuy; Huyen Tran, Thi Thu; Chu, Van Tuan; Thinh Tran, Quang; Tuan Mai, Anh

2017-09-01

The carbon nanotubes field effect transistor (CNTFET) based DNA sensor was developed, in this paper, for detection of influenza A virus DNA. Number of factors that influence the output signal and analytical results were investigated. The initial probe DNA, decides the available DNA strands on CNTs, was 10 μM. The hybridization time for defined single helix was 120 min. The hybridization temperature was set at 30 °C to get a net change in drain current of the DNA sensor without altering properties of any biological compounds. The response time of the DNA sensor was less than one minute with a high reproducibility. In addition, the DNA sensor has a wide linear detection range from 1 pM to 10 nM, and a very low detection limit of 1 pM. Finally, after 7-month storage in 7.4 pH buffer, the output signal of DNA sensor recovered 97%.

Evaluation of Electromechanical Systems Dynamically Emulating a Candidate Hydrokinetic Turbine

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cavagnaro, Robert J.; Neely, Jason C.; Fay, Franois-Xavier

The use of controllable motor-generator sets to emulate the dynamics of a hydrokinetic turbine is evaluated as an alternative to field testing a prototype. The emulator control dynamic equations are presented, methods for scaling turbine parameters are examined, and experimental results are presented from three electromechanical emulation machines (EEMs) programmed to emulate the same vertical-axis fixed-pitch turbine. Although hardware platforms and control implementations varied, results show that each EEM is successful in emulating the turbine model, thus demonstrating the general feasibility of the approach. However, performance of motor control under torque command, current command or speed command differed. In onemore » of the EEMs evaluated, the power take off controller tracks the maximum power-point of the turbine in response to turbulence. Utilizing realistic inflow conditions and control laws, the emulator dynamic speed response is shown to agree well at low frequencies with numerical simulation but to deviate at high frequencies.« less

A Virtual Information-Action Workspace for Command and Control

NASA Astrophysics Data System (ADS)

Lintern, Gavan; Naikar, Neelam

2002-10-01

Information overload has become a critical challenge within military Command and Control. However, the problem is not so much one of too much information but of abundant information that is poorly organized and poorly represented. In addition, the capabilities to test the effects of decisions before they are implemented and to monitor the progress of events after a decision is implemented are primitive. A virtual information-action workspace could be designed to resolve these issues. The design of such a space would require a detailed understanding of the specific information needed to support decision making in Command and Control. That information can be obtained with the use of knowledge acquisition and knowledge representation tools from the field of applied cognitive psychology. In addition, it will be necessary to integrate forms for perception and action into a virtual space that will support access to the information and that will provide means for testing and implementing decisions. This paper presents a rationale for a virtual information-action workspace and outlines an approach to its design.

A Computational Model of Spatial Development

NASA Astrophysics Data System (ADS)

Hiraki, Kazuo; Sashima, Akio; Phillips, Steven

Psychological experiments on children's development of spatial knowledge suggest experience at self-locomotion with visual tracking as important factors. Yet, the mechanism underlying development is unknown. We propose a robot that learns to mentally track a target object (i.e., maintaining a representation of an object's position when outside the field-of-view) as a model for spatial development. Mental tracking is considered as prediction of an object's position given the previous environmental state and motor commands, and the current environment state resulting from movement. Following Jordan & Rumelhart's (1992) forward modeling architecture the system consists of two components: an inverse model of sensory input to desired motor commands; and a forward model of motor commands to desired sensory input (goals). The robot was tested on the `three cups' paradigm (where children are required to select the cup containing the hidden object under various movement conditions). Consistent with child development, without the capacity for self-locomotion the robot's errors are self-center based. When given the ability of self-locomotion the robot responds allocentrically.

Evaluation of Electromechanical Systems Dynamically Emulating a Candidate Hydrokinetic Turbine

DOE PAGES

Cavagnaro, Robert J.; Neely, Jason C.; Fay, Franois-Xavier; ...

2016-11-06

The use of controllable motor-generator sets to emulate the dynamics of a hydrokinetic turbine is evaluated as an alternative to field testing a prototype. The emulator control dynamic equations are presented, methods for scaling turbine parameters are examined, and experimental results are presented from three electromechanical emulation machines (EEMs) programmed to emulate the same vertical-axis fixed-pitch turbine. Although hardware platforms and control implementations varied, results show that each EEM is successful in emulating the turbine model, thus demonstrating the general feasibility of the approach. However, performance of motor control under torque command, current command or speed command differed. In onemore » of the EEMs evaluated, the power take off controller tracks the maximum power-point of the turbine in response to turbulence. Utilizing realistic inflow conditions and control laws, the emulator dynamic speed response is shown to agree well at low frequencies with numerical simulation but to deviate at high frequencies.« less

The K9 On-Board Rover Architecture

NASA Technical Reports Server (NTRS)

Bresina, John L.; Bualat, Maria; Fair, Michael; Washington, Richard; Wright, Anne

2006-01-01

This paper describes the software architecture of NASA Ames Research Center s K9 rover. The goal of the onboard software architecture team was to develop a modular, flexible framework that would allow both high- and low-level control of the K9 hardware. Examples of low-level control are the simple drive or pan/tilt commands which are handled by the resource managers, and examples of high-level control are the command sequences which are handled by the conditional executive. In between these two control levels are complex behavioral commands which are handled by the pilot, such as drive to goal with obstacle avoidance or visually servo to a target. This paper presents the design of the architecture as of Fall 2000. We describe the state of the architecture implementation as well as its current evolution. An early version of the architecture was used for K9 operations during a dual-rover field experiment conducted by NASA Ames Research Center (ARC) and the Jet Propulsion Laboratory (JPL) from May 14 to May 16, 2000.

Decomposition and control of complex systems - Application to the analysis and control of industrial and economic systems /energy production/ with limited supplies

NASA Astrophysics Data System (ADS)

de Coligny, M.

Optimized control strategies are developed for industrial installations where many variables of energy supply and storage are involved, with a particular focus on characteristics of a solar central tower power plant. It is shown that optimal regulation resides in controlling all disturbances which occur in a limited domain of the entire system, using robust control schemes. Choosing a command is then dependent on defining precise operational limits as constraints on the machines' performances. Attention is given to the development of variational principles used for the elements of the command logic. Particular consideration is given to a limited supply in storage in spatial and temporal terms. Commands for alterations in functions are then available on-line, and discontinuities are not a feature of the control system. The strategy is applied to the case of a field of heliostats and a central tower themal receiver showing that management is possible on the basis of a sliding horizon.

Team Leader Structuring for Team Effectiveness and Team Learning in Command-and-Control Teams

PubMed Central

van der Haar, Selma; Koeslag-Kreunen, Mieke; Euwe, Eline; Segers, Mien

2017-01-01

Due to their crucial and highly consequential task, it is of utmost importance to understand the levers leading to effectiveness of multidisciplinary emergency management command-and-control (EMCC) teams. We argue that the formal EMCC team leader needs to initiate structure in the team meetings to support organizing the work as well as facilitate team learning, especially the team learning process of constructive conflict. In a sample of 17 EMCC teams performing a realistic EMCC exercise, including one or two team meetings (28 in sum), we coded the team leader’s verbal structuring behaviors (1,704 events), rated constructive conflict by external experts, and rated team effectiveness by field experts. Results show that leaders of effective teams use structuring behaviors more often (except asking procedural questions) but decreasingly over time. They support constructive conflict by clarifying and by making summaries that conclude in a command or decision in a decreasing frequency over time. PMID:28490856

DSSR-enhanced visualization of nucleic acid structures in Jmol.

PubMed

Hanson, Robert M; Lu, Xiang-Jun

2017-07-03

Sophisticated and interactive visualizations are essential for making sense of the intricate 3D structures of macromolecules. For proteins, secondary structural components are routinely featured in molecular graphics visualizations. However, the field of RNA structural bioinformatics is still lagging behind; for example, current molecular graphics tools lack built-in support even for base pairs, double helices, or hairpin loops. DSSR (Dissecting the Spatial Structure of RNA) is an integrated and automated command-line tool for the analysis and annotation of RNA tertiary structures. It calculates a comprehensive and unique set of features for characterizing RNA, as well as DNA structures. Jmol is a widely used, open-source Java viewer for 3D structures, with a powerful scripting language. JSmol, its reincarnation based on native JavaScript, has a predominant position in the post Java-applet era for web-based visualization of molecular structures. The DSSR-Jmol integration presented here makes salient features of DSSR readily accessible, either via the Java-based Jmol application itself, or its HTML5-based equivalent, JSmol. The DSSR web service accepts 3D coordinate files (in mmCIF or PDB format) initiated from a Jmol or JSmol session and returns DSSR-derived structural features in JSON format. This seamless combination of DSSR and Jmol/JSmol brings the molecular graphics of 3D RNA structures to a similar level as that for proteins, and enables a much deeper analysis of structural characteristics. It fills a gap in RNA structural bioinformatics, and is freely accessible (via the Jmol application or the JSmol-based website http://jmol.x3dna.org). © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Free-solution electrophoretic separations of DNA–drag-tag conjugates on glass microchips with no polymer network and no loss of resolution at increased electric field strength

PubMed Central

Albrecht, Jennifer Coyne; Kerby, Matthew B.; Niedringhaus, Thomas P.; Lin, Jennifer S.; Wang, Xiaoxiao; Barron, Annelise E.

2012-01-01

Here, we demonstrate the potential for high-resolution electrophoretic separations of ssDNA-protein conjugates in borosilicate glass microfluidic chips, with no sieving media and excellent repeatability. Using polynucleotides of two different lengths conjugated to moderately cationic protein polymer drag-tags, we measured separation efficiency as a function of applied electric field. In excellent agreement with prior theoretical predictions of Slater et al., resolution is found to remain constant as applied field is increased up to 700 V/cm, the highest field we were able to apply. This remarkable result illustrates the fundamentally different physical limitations of Free-Solution Conjugate Electrophoresis (FSCE)-based DNA separations relative to matrix-based DNA electrophoresis. Single-stranded DNA separations in “gels” have always shown rapidly declining resolution as the field strength is increased; this is especially true for ssDNA > 400 bases in length. FSCE’s ability to decouple DNA peak resolution from applied electric field suggests the future possibility of ultra-rapid FSCE sequencing on chips. We investigated sources of peak broadening for FSCE separations on borosilicate glass microchips, using six different protein polymer drag-tags. For drag-tags with four or more positive charges, electrostatic and adsorptive interactions with pHEA-coated microchannel walls led to appreciable band-broadening, while much sharper peaks were seen for bioconjugates with nearly charge-neutral protein drag-tags. PMID:21500207

One simple DNA extraction device and its combination with modified visual loop-mediated isothermal amplification for rapid on-field detection of genetically modified organisms.

PubMed

Zhang, Miao; Liu, Yinan; Chen, Lili; Quan, Sheng; Jiang, Shimeng; Zhang, Dabing; Yang, Litao

2013-01-02

Quickness, simplicity, and effectiveness are the three major criteria for establishing a good molecular diagnosis method in many fields. Herein we report a novel detection system for genetically modified organisms (GMOs), which can be utilized to perform both on-field quick screening and routine laboratory diagnosis. In this system, a newly designed inexpensive DNA extraction device was used in combination with a modified visual loop-mediated isothermal amplification (vLAMP) assay. The main parts of the DNA extraction device included a silica gel membrane filtration column and a modified syringe. The DNA extraction device could be easily operated without using other laboratory instruments, making it applicable to an on-field GMO test. High-quality genomic DNA (gDNA) suitable for polymerase chain reaction (PCR) and isothermal amplification could be quickly isolated from plant tissues using this device within 15 min. In the modified vLAMP assay, a microcrystalline wax encapsulated detection bead containing SYBR green fluorescent dye was introduced to avoid dye inhibition and cross-contaminations from post-LAMP operation. The system was successfully applied and validated in screening and identification of GM rice, soybean, and maize samples collected from both field testing and the Grain Inspection, Packers, and Stockyards Administration (GIPSA) proficiency test program, which demonstrated that it was well-adapted to both on-field testing and/or routine laboratory analysis of GMOs.

An Evaluation of the Frequency and Severity of Motion Sickness Incidences in Personnel Within the Command and Control Vehicle (C2V)

NASA Technical Reports Server (NTRS)

Cowings, Patricia S.; Toscano, William B.; DeRoshia, Charles

1998-01-01

The purpose of this study was to assess the frequency and severity of motion sickness in personnel during a field exercise in the Command and Control Vehicle (C2V). This vehicle contains four workstations where military personnel are expected to perform command decisions in the field during combat conditions. Eight active duty military men (U.S. Army) at the Yuma Proving Grounds in Arizona participated in this study. All subjects were given baseline performance tests while their physiological responses were monitored on the first day. On the second day of their participation, subjects rode in the C2V while their physiological responses and performance measures were recorded. Self-reports of motion sickness were also recorded. Results showed that only one subject experienced two incidences of emesis. However, seven out of the eight subjects reported other motion sickness symptoms; most predominant was the report of drowsiness, which occurred a total of 19 times. Changes in physiological responses were observed relative to motion sickness symptoms reported and the different environmental conditions (i.e., level, hills, gravel) during the field exercise. Performance data showed an overall decrement during the C2V exercise. These findings suggest that malaise and severe drowsiness can potentially impact the operational efficiency of the C2V crew. It was concluded that conflicting sensory information from the subject's visual displays and movements of the vehicle during the field exercise significantly contributed to motion sickness symptoms. It was recommended that a second study be conducted to further evaluate the impact of seat position or orientation and C2V experience on motion sickness susceptibility. Further, it was recommended that an investigation be performed on behavioral methods for improving crew alertness, motivation, and performance and for reducing malaise.

Ni-DNA-based nanowires and nanodevices

NASA Astrophysics Data System (ADS)

Chang, Chia-Ching; Yuan, Chiun-Jye; Jian, Wen-Bin; Chen, Yu-Chang; di Ventra, Massimiliano

DNA is a highly versatile biopolymer that has been a recent focus in the field of nanomachines and nanoelectronics. DNA exhibits high stability, adjustable conductance, self-organizing capability, programmability and vast information storage. It is an ideal material in the applications of nanodevices, nanoelectronics, and molecular computing. Low conductance of native DNA renders applications difficult. However, doping with nickel ions tunes the DNA into a conducting polymer. Further studies showed that nickel ions containing DNA (Ni-DNA) nanowires exhibit characteristics of memristor and memcapacitor making them a potential mass information storage system. In summary, Ni-DNA has promising applications in a variety of fields, including nanoelectronics, biosensors and memcomputing. This study was supported in part by the Ministry of Science and Technology (MOST), Taiwan (ROC) MOST 103-2112-M-009-011 -MY3, and MOST 105-2627-M-009-006.

Comparison of Haemophilus parasuis reference strains and field isolates by using random amplified polymorphic DNA and protein profiles

PubMed Central

2012-01-01

Background Haemophilus parasuis is the causative agent of Glässer’s disease and is a pathogen of swine in high-health status herds. Reports on serotyping of field strains from outbreaks describe that approximately 30% of them are nontypeable and therefore cannot be traced. Molecular typing methods have been used as alternatives to serotyping. This study was done to compare random amplified polymorphic DNA (RAPD) profiles and whole cell protein (WCP) lysate profiles as methods for distinguishing H. parasuis reference strains and field isolates. Results The DNA and WCP lysate profiles of 15 reference strains and 31 field isolates of H. parasuis were analyzed using the Dice and neighbor joining algorithms. The results revealed unique and reproducible DNA and protein profiles among the reference strains and field isolates studied. Simpson’s index of diversity showed significant discrimination between isolates when three 10mer primers were combined for the RAPD method and also when both the RAPD and WCP lysate typing methods were combined. Conclusions The RAPD profiles seen among the reference strains and field isolates did not appear to change over time which may reflect a lack of DNA mutations in the genes of the samples. The recent field isolates had different WCP lysate profiles than the reference strains, possibly because the number of passages of the type strains may affect their protein expression. PMID:22703293

Dielectrophoretic Isolation and Detection of cfc-DNA Nanoparticulate Biomarkers and Virus from Blood

PubMed Central

Sonnenberg, Avery; Marciniak, Jennifer Y.; McCanna, James; Krishnan, Rajaram; Rassenti, Laura; Kipps, Thomas J.; Heller, Michael J.

2015-01-01

Dielectrophoretic (DEP) microarray devices allow important cellular nanoparticulate biomarkers and virus to be rapidly isolated, concentrated and detected directly from clinical and biological samples. A variety of sub-micron nanoparticulate entities including cell free circulating (cfc) DNA, mitochondria and virus can be isolated into DEP high-field areas on microelectrodes, while blood cells and other micron-size entities become isolated into DEP low-field areas between the microelectrodes. The nanoparticulate entities are held in the DEP high-field areas while cells are washed away along with proteins and other small molecules which are not affected by the DEP electric fields. DEP carried out on 20 µL of whole blood obtained from Chronic Lymphocytic Leukemia (CLL) patients showed a considerable amount of SYBR Green stained DNA fluorescent material concentrated in the DEP high-field regions. Whole blood obtained from healthy individuals showed little or no fluorescent DNA materials in the DEP high-field regions. Fluorescent T7 bacteriophage virus could be isolated directly from blood samples, and fluorescently stained mitochondria could be isolated from biological buffer samples. Using newer DEP microarray devices, high molecular weight (hmw) DNA could be isolated from serum and detected at levels as low as 8–16 ng/mL. PMID:23436471

Electrostatic coupling between DNA and its counterions modulates the observed translational diffusion coefficients.

PubMed

Stellwagen, Earle; Stellwagen, Nancy C

2015-09-01

Free solution capillary electrophoresis (CE) is a useful technique for measuring the translational diffusion coefficients of charged analytes. The measurements are relatively fast if the polarity of the electric field is reversed to drive the analyte back and forth past the detection window during each run. We have tested the validity of the resulting diffusion coefficients using double-stranded DNA molecules ranging in size from 20 to 960 base pairs as the model system. The diffusion coefficients of small DNAs are equal to values in the literature measured by other techniques. However, the diffusion coefficients of DNA molecules larger than ∼30 base pairs are anomalously high and deviate increasingly from the literature values with increasing DNA molar mass. The anomalously high diffusion coefficients are due to electrostatic coupling between the DNA and its counterions. As a result, the measured diffusion coefficients vary with the diffusion coefficient of the counterion, as well as with cation concentration and electric field strength. These effects can be reduced or eliminated by measuring apparent diffusion coefficients of the DNA at several different electric field strengths and extrapolating the results to zero electric field.

Tilted hexagonal post arrays: DNA electrophoresis in anisotropic media.

PubMed

Chen, Zhen; Dorfman, Kevin D

2014-02-01

Using Brownian dynamics simulations, we show that DNA electrophoresis in a hexagonal array of micron-sized posts changes qualitatively when the applied electric field vector is not coincident with the lattice vectors of the array. DNA electrophoresis in such "tilted" post arrays is superior to the standard "un-tilted" approach; while the time required to achieve a resolution of unity in a tilted post array is similar to an un-tilted array at a low-electric field strengths, this time (i) decreases exponentially with electric field strength in a tilted array and (ii) increases exponentially with electric field strength in an un-tilted array. Although the DNA dynamics in a post array are complicated, the electrophoretic mobility results indicate that the "free path," i.e. the average distance of ballistic trajectories of point-sized particles launched from random positions in the unit cell until they intersect the next post, is a useful proxy for the detailed DNA trajectories. The analysis of the free path reveals a fundamental connection between anisotropy of the medium and DNA transport therein that goes beyond simply improving the separation device. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Simulations of DNA stretching by flow field in microchannels with complex geometry.

PubMed

Huang, Chiou-De; Kang, Dun-Yen; Hsieh, Chih-Chen

2014-01-01

Recently, we have reported the experimental results of DNA stretching by flow field in three microchannels (C. H. Lee and C. C. Hsieh, Biomicrofluidics 7(1), 014109 (2013)) designed specifically for the purpose of preconditioning DNA conformation for easier stretching. The experimental results do not only demonstrate the superiority of the new devices but also provides detailed observation of DNA behavior in complex flow field that was not available before. In this study, we use Brownian dynamics-finite element method (BD-FEM) to simulate DNA behavior in these microchannels, and compare the results against the experiments. Although the hydrodynamic interaction (HI) between DNA segments and between DNA and the device boundaries was not included in the simulations, the simulation results are in fairly good agreement with the experimental data from either the aspect of the single molecule behavior or from the aspect of ensemble averaged properties. The discrepancy between the simulation and the experimental results can be explained by the neglect of HI effect in the simulations. Considering the huge savings on the computational cost from neglecting HI, we conclude that BD-FEM can be used as an efficient and economic designing tool for developing new microfluidic device for DNA manipulation.

Improved model of hydrated calcium ion for molecular dynamics simulations using classical biomolecular force fields.

PubMed

Yoo, Jejoong; Wilson, James; Aksimentiev, Aleksei

2016-10-01

Calcium ions (Ca(2+) ) play key roles in various fundamental biological processes such as cell signaling and brain function. Molecular dynamics (MD) simulations have been used to study such interactions, however, the accuracy of the Ca(2+) models provided by the standard MD force fields has not been rigorously tested. Here, we assess the performance of the Ca(2+) models from the most popular classical force fields AMBER and CHARMM by computing the osmotic pressure of model compounds and the free energy of DNA-DNA interactions. In the simulations performed using the two standard models, Ca(2+) ions are seen to form artificial clusters with chloride, acetate, and phosphate species; the osmotic pressure of CaAc2 and CaCl2 solutions is a small fraction of the experimental values for both force fields. Using the standard parameterization of Ca(2+) ions in the simulations of Ca(2+) -mediated DNA-DNA interactions leads to qualitatively wrong outcomes: both AMBER and CHARMM simulations suggest strong inter-DNA attraction whereas, in experiment, DNA molecules repel one another. The artificial attraction of Ca(2+) to DNA phosphate is strong enough to affect the direction of the electric field-driven translocation of DNA through a solid-state nanopore. To address these shortcomings of the standard Ca(2+) model, we introduce a custom model of a hydrated Ca(2+) ion and show that using our model brings the results of the above MD simulations in quantitative agreement with experiment. Our improved model of Ca(2+) can be readily applied to MD simulations of various biomolecular systems, including nucleic acids, proteins and lipid bilayer membranes. © 2016 Wiley Periodicals, Inc. Biopolymers 105: 752-763, 2016. © 2016 Wiley Periodicals, Inc.

DNA electrophoresis in agarose gels: effects of field and gel concentration on the exponential dependence of reciprocal mobility on DNA length.

PubMed

Rill, Randolph L; Beheshti, Afshin; Van Winkle, David H

2002-08-01

Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equation nu(L) = (beta+alpha(1+exp(-L/gamma))(-1) can be used to relate mobility to length with high accuracy. Mobility data were fit with chi(2) > 0.999 for all gel concentrations and fields ranging from 2.5 to 5 V/cm, and for lower fields at low gel concentrations. Analyses using so-called reptation plots (Rousseau, J., Drouin, G., Slater, G. W., Phys. Rev. Lett. 1997, 79, 1945-1948) indicate that this simple exponential relation is obeyed well when there is a smooth transition from the Ogston sieving regime to the reptation regime with increasing DNA length. Deviations from this equation occur when DNA migration is hindered, apparently by entropic-trapping, which is favored at low fields and high gel concentrations in the ranges examined.

All-Atom Polarizable Force Field for DNA Based on the Classical Drude Oscillator Model

PubMed Central

Savelyev, Alexey; MacKerell, Alexander D.

2014-01-01

Presented is a first generation atomistic force field for DNA in which electronic polarization is modeled based on the classical Drude oscillator formalism. The DNA model is based on parameters for small molecules representative of nucleic acids, including alkanes, ethers, dimethylphosphate, and the nucleic acid bases and empirical adjustment of key dihedral parameters associated with the phosphodiester backbone, glycosidic linkages and sugar moiety of DNA. Our optimization strategy is based on achieving a compromise between satisfying the properties of the underlying model compounds in the gas phase targeting QM data and reproducing a number of experimental properties of DNA duplexes in the condensed phase. The resulting Drude force field yields stable DNA duplexes on the 100 ns time scale and satisfactorily reproduces (1) the equilibrium between A and B forms of DNA and (2) transitions between the BI and BII sub-states of B form DNA. Consistency with the gas phase QM data for the model compounds is significantly better for the Drude model as compared to the CHARMM36 additive force field, which is suggested to be due to the improved response of the model to changes in the environment associated with the explicit inclusion of polarizability. Analysis of dipole moments associated with the nucleic acid bases shows the Drude model to have significantly larger values than those present in CHARMM36, with the dipoles of individual bases undergoing significant variations during the MD simulations. Additionally, the dipole moment of water was observed to be perturbed in the grooves of DNA. PMID:24752978

All-Atom MD Simulation of DNA Condensation Using Ab Initio Derived Force Field Parameters of Cobalt(III)-Hexammine.

PubMed

Sun, Tiedong; Mirzoev, Alexander; Korolev, Nikolay; Lyubartsev, Alexander P; Nordenskiöld, Lars

2017-08-24

It is well established that the presence of the trivalent cobalt(III)-hexammine cation (CoHex 3+ ) at submillimolar concentrations leads to bundling (condensation) of double-stranded DNA molecules, which is caused by DNA-DNA attraction induced by the multivalent counterions. However, the detailed mechanism of this process is still not fully understood. Furthermore, in all-atom molecular dynamics (MD) simulations, spontaneous aggregation of several DNA oligonucleotides in the presence of CoHex 3+ has previously not been demonstrated. In order to obtain a rigorous description of CoHex 3+ -nucleic acid interactions and CoHex 3+ -induced DNA condensation to be used in MD simulations, we have derived optimized force field parameters of the CoHex 3+ ion. They were obtained from Car-Parrinello molecular dynamics simulation of a single CoHex 3+ ion in the presence of 125 water molecules. The new set of force field parameters reproduces the experimentally known transition of DNA from B- to A-form, and qualitatively describes changes of DNA and RNA persistence lengths. We then carried out a 2 μs long atomistic simulation of four DNA oligomers each consisting of 36 base pairs in the presence of CoHex 3+ . We demonstrate that, in this system, DNA molecules display attractive interactions and aggregate into bundle-like structures. This behavior depends critically on the details of the CoHex 3+ interaction with DNA. A control simulation with a similar setup but in the presence of Mg 2+ does not induce DNA-DNA attraction, which is also in agreement with experiment.

Chondroitin sulfate-polyethylenimine copolymer-coated superparamagnetic iron oxide nanoparticles as an efficient magneto-gene carrier for microRNA-encoding plasmid DNA delivery.

PubMed

Lo, Yu-Lun; Chou, Han-Lin; Liao, Zi-Xian; Huang, Shih-Jer; Ke, Jyun-Han; Liu, Yu-Sheng; Chiu, Chien-Chih; Wang, Li-Fang

2015-05-14

MicroRNA-128 (miR-128) is an attractive therapeutic molecule with powerful glioblastoma regulation properties. However, miR-128 lacks biological stability and leads to poor delivery efficacy in clinical applications. In our previous study, we demonstrated two effective transgene carriers, including polyethylenimine (PEI)-decorated superparamagnetic iron oxide nanoparticles (SPIONs) as well as chemically-conjugated chondroitin sulfate-PEI copolymers (CPs). In this contribution, we report optimized conditions for coating CPs onto the surfaces of SPIONs, forming CPIOs, for magneto-gene delivery systems. The optimized weight ratio of the CPs and SPIONs is 2 : 1, which resulted in the formation of a stable particle as a good transgene carrier. The hydrodynamic diameter of the CPIOs is ∼136 nm. The gel electrophoresis results demonstrate that the weight ratio of CPIO/DNA required to completely encapsulate pDNA is ≥3. The in vitro tests of CPIO/DNA were done in 293 T, CRL5802, and U87-MG cells in the presence and absence of an external magnetic field. The magnetofection efficiency of CPIO/DNA was measured in the three cell lines with or without fetal bovine serum (FBS). CPIO/DNA exhibited remarkably improved gene expression in the presence of the magnetic field and 10% FBS as compared with a gold non-viral standard, PEI/DNA, and a commercial magnetofection reagent, PolyMag/DNA. In addition, CPIO/DNA showed less cytotoxicity than PEI/DNA and PolyMag/DNA against the three cell lines. The transfection efficiency of the magnetoplex improved significantly with an assisted magnetic field. In miR-128 delivery, a microRNA plate array and fluorescence in situ hybridization were used to demonstrate that CPIO/pMIRNA-128 indeed expresses more miR-128 with the assisted magnetic field than without. In a biodistribution test, CPIO/Cy5-DNA showed higher accumulation at the tumor site where an external magnet is placed nearby.

Regulation of DNA conformations and dynamics in flows with hybrid field microfluidics.

PubMed

Ren, Fangfang; Zu, Yingbo; Kumar Rajagopalan, Kartik; Wang, Shengnian

2012-01-01

Visualizing single DNA dynamics in flow provides a wealth of physical insights in biophysics and complex flow study. However, large signal fluctuations, generated from diversified conformations, deformation history dependent dynamics and flow induced stochastic tumbling, often frustrate its wide adoption in single molecule and polymer flow study. We use a hybrid field microfluidic (HFM) approach, in which an electric field is imposed at desired locations and appropriate moments to balance the flow stress on charged molecules, to effectively regulate the initial conformations and the deformation dynamics of macromolecules in flow. With λ-DNA and a steady laminar shear flow as the model system, we herein studied the performance of HFM on regulating DNA trapping, relaxation, coil-stretch transition, and accumulation. DNA molecules were found to get captured in the focused planes when motions caused by flow, and the electric field were balanced. The trapped macromolecules relaxed in two different routes while eventually became more uniform in size and globule conformations. When removing the electric field, the sudden stretching dynamics of DNA molecules exhibited a more pronounced extension overshoot in their transient response under a true step function of flow stress while similar behaviors to what other pioneering work in steady shear flow. Such regulation strategies could be useful to control the conformations of other important macromolecules (e.g., proteins) and help better reveal their molecular dynamics.

Command History 1970. Volume 2. Sanitized

DTIC Science & Technology

1970-01-01

included authorizations for physicians, dentists, pharmacists , and other specialists- the clinical field was short 232 physicians. (S) By 1973 the...progrztm for lmpeiomentativil and designated the CEIMP as Uti - stirglo source document ior ItVNA V C -E: W&L.I1 Vietnanlization of US Advisor Cocrn

Field of Vision - Vehicles

DTIC Science & Technology

2017-05-11

NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) US Army Aberdeen Test Center (TEDT-AT-WFS) 400...AND ADDRESS(ES) Policy and Standardization Division (CSTE-TM) U.S. Army Test and Evaluation Command 2202 Aberdeen Boulevard Aberdeen Proving Ground

32 CFR 806b.7 - Responsibilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... is the senior Air Force Privacy Official with overall responsibility for the Air Force Privacy Act... Data Integrity Board. (5) Provides guidance and assistance to Major Commands, field operating agencies... validate currency. (6) Evaluate the health of the program at regular intervals using this part as guidance...

MSIAC’s M&S Journal Online: Volume 2, September 2006

DTIC Science & Technology

2006-09-01

engineering fields, such as mechanical and electrical , rely on composability and interchangeability (substitutability) of components into workable systems...Terrain Data for the Vector-in-Command and Engineer Functional Area Models”, Technical Report GL-90-9, September 1990, USAEWES, Vicksburg 8. Frankenstein

The Megabyte Will Always Get Through

DTIC Science & Technology

2012-05-17

Curry , the commander of the Army Air Forces Tactical School, wanted the school at Maxwell Field to be a “clearing house where tactical ideas can flow...intelligence, spy satellites, drone aircraft, and manned bombers, could identify the target. This cyber attack once more demonstrated “the

An Approach to Command and Control Using Emerging Technologies

DTIC Science & Technology

2013-06-01

communicating in disadvantaged networks like fielded tactical radio networks. With the emergence and proliferation of new tactical radios capable of...undesirable, particularly on disadvantaged radio links, so in the return CoT message HTTP links are provided so the client may download the chips

Navy MANTECH Program Fiscal Year 2002 Annual Report

DTIC Science & Technology

2003-04-01

Journal of Materials Engineering and Performance, Vol. 10, No 6. K. Kannan and J. J. Valencia Field-Scale Demonstration of Electrocoagulation and...Naval Air Systems Command / NADEP CP $741,000 Abrasive Flow Machining (coatings removal ) evaluation for repair of F404 turbine blade engine

32 CFR 644.137 - Maneuver agreements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... planning and acquires rights to use land and other facilities for Department of the Army exercises. The... and, after the maneuver is completed, will be responsible for negotiating restoration settlements and... director at field level whereby the command will assume responsibility for settlement of real estate...

The Mobile Agents Integrated Field Test: Mars Desert Research Station April 2003

NASA Technical Reports Server (NTRS)

Clancey, William J.; Sierhuis, Maarten; Alena, Rick; Crawford, Sekou; Dowding, John; Graham, Jeff; Kaskiris, Charis; Tyree, Kim S.; vanHoof, Ron

2003-01-01

The Mobile Agents model-based, distributed architecture, which integrates diverse components in a system for lunar and planetary surface operations, was extensively tested in a two-week field "technology retreat" at the Mars Society s Desert Research Station (MDRS) during April 2003. More than twenty scientists and engineers from three NASA centers and two universities refined and tested the system through a series of incremental scenarios. Agent software, implemented in runtime Brahms, processed GPS, health data, and voice commands-monitoring, controlling and logging science data throughout simulated EVAs with two geologists. Predefined EVA plans, modified on the fly by voice command, enabled the Mobile Agents system to provide navigation and timing advice. Communications were maintained over five wireless nodes distributed over hills and into canyons for 5 km; data, including photographs and status was transmitted automatically to the desktop at mission control in Houston. This paper describes the system configurations, communication protocols, scenarios, and test results.

DNA Polymerase Eta and Chemotherapeutic Agents

PubMed Central

2011-01-01

Abstract The discovery of human DNA polymerase eta (pol η) has a major impact on the fields of DNA replication/repair fields. Since the discovery of human pol η, a number of new DNA polymerases with the ability to bypass various DNA lesions have been discovered. Among these polymerases, pol η is the most extensively studied lesion bypass polymerase with a defined major biological function, that is, to replicate across the cyclobutane pyrimidine dimers introduced by UV irradiation. Cyclobutane pyrimidine dimer is a major DNA lesion that causes distortion of DNA structure and block the replicative DNA polymerases during DNA replication process. Genetic defects in the pol η gene, Rad30, results in a disease called xeroderma pigmentosum variant. This review focuses on the overall properties of pol η and the mechanism that involved in regulating its activity in cells. In addition, the role of pol η in the action of DNA-targeting anticancer compounds is also discussed. Antioxid. Redox Signal. 14, 2521–2529. PMID:21050139

Divergent dispersion behavior of ssDNA fragments during microchip electrophoresis in pDMA and LPA entangled polymer networks

PubMed Central

Fredlake, Christopher P.; Hert, Daniel G.; Niedringhaus, Thomas P.; Lin, Jennifer S.; Barron, Annelise E.

2015-01-01

Resolution of DNA fragments separated by electrophoresis in polymer solutions (“matrices”) is determined by both the spacing between peaks and the width of the peaks. Prior research on the development of high-performance separation matrices has been focused primarily on optimizing DNA mobility and matrix selectivity, and gave less attention to peak broadening. Quantitative data are rare for peak broadening in systems in which high electric field strengths are used (> 150 V/cm), which is surprising since capillary and microchip-based systems commonly run at these field strengths. Here, we report results for a study of band broadening behavior for ssDNA fragments on a glass microfluidic chip, for electric field strengths up to 320 V/cm. We compare dispersion coefficients obtained in a poly(N,N-dimethylacrylamide) (pDMA) separation matrix that was developed for chip-based DNA sequencing with a commercially available linear polyacrylamide (LPA) matrix commonly used in capillaries. Much larger DNA dispersion coefficients were measured in the LPA matrix as compared to the pDMA matrix, and the dependences of dispersion coefficient on DNA size and electric field strength were found to differ quite starkly in the two matrices. These observations lead us to propose that DNA migration mechanisms differ substantially in our custom pDMA matrix compared to the commercially available LPA matrix. We discuss the implications of these results in terms of developing optimal matrices for specific separation (microchip or capillary) platforms. PMID:22648809

STS-111 & Expedition 4 Crew Return Ceremony

NASA Image and Video Library

2002-06-24

JSC2002-E-26021 (21 June 2002) --- Johnson Space Center’s (JSC) Director Jefferson Davis Howell, Jr. speaks from the lectern in Hangar 990 at Ellington Field during the STS-111 and Expedition Four crew return ceremonies. Seated (from left) are General Vasily Tsiblyiev, Deputy Director of the Gagarin Cosmonaut Training Center; cosmonaut Yury I. Onufrienko, Expedition Four mission commander; astronauts Carl E. Walz and Daniel W. Bursch, both Expedition Four flight engineers; NASA Administrator Sean O'Keefe; astronauts Kenneth D. Cockrell, STS-111 mission commander; Paul S. Lockhart, pilot; Franklin R. Chang-Diaz and Philippe Perrin, both mission specialists. Tsiblyiev and Onufrienko represent Rosaviakosmos, and Perrin represents CNES, the French Space Agency.

Robot Command Interface Using an Audio-Visual Speech Recognition System

NASA Astrophysics Data System (ADS)

Ceballos, Alexánder; Gómez, Juan; Prieto, Flavio; Redarce, Tanneguy

In recent years audio-visual speech recognition has emerged as an active field of research thanks to advances in pattern recognition, signal processing and machine vision. Its ultimate goal is to allow human-computer communication using voice, taking into account the visual information contained in the audio-visual speech signal. This document presents a command's automatic recognition system using audio-visual information. The system is expected to control the laparoscopic robot da Vinci. The audio signal is treated using the Mel Frequency Cepstral Coefficients parametrization method. Besides, features based on the points that define the mouth's outer contour according to the MPEG-4 standard are used in order to extract the visual speech information.

Environmental DNA as a Tool for Inventory and Monitoring of Aquatic Vertebrates

DTIC Science & Technology

2017-07-01

geomorphic calculations and description of each reach. Methods Channel Surveys We initially selected reaches based on access and visual indicators...WA 99164 I-2 Environmental DNA lab protocol: designing species-specific qPCR assays Species-specific surveys should use quantitative polymerase...to traditional field sampling with respect to sensitivity, detection probabilities, and cost efficiency. Compared to field surveys , eDNA sampling

High mobility organic field-effect transistor based on water-soluble deoxyribonucleic acid via spray coating

NASA Astrophysics Data System (ADS)

Shi, Wei; Han, Shijiao; Huang, Wei; Yu, Junsheng

2015-01-01

High mobility organic field-effect transistors (OFETs) by inserting water-soluble deoxyribonucleic acid (DNA) buffer layer between electrodes and pentacene film through spray coating process were fabricated. Compared with the OFETs incorporated with DNA in the conventional organic solvents of ethanol and methanol: water mixture, the water-soluble DNA based OFET exhibited an over four folds enhancement of field-effect mobility from 0.035 to 0.153 cm2/Vs. By characterizing the surface morphology and the crystalline structure of pentacene active layer through atomic force microscope and X-ray diffraction, it was found that the adoption of water solvent in DNA solution, which played a key role in enhancing the field-effect mobility, was ascribed to both the elimination of the irreversible organic solvent-induced bulk-like phase transition of pentacene film and the diminution of a majority of charge trapping at interfaces in OFETs.

Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors

NASA Astrophysics Data System (ADS)

Star, Alexander; Tu, Eugene; Niemann, Joseph; Gabriel, Jean-Christophe P.; Joiner, C. Steve; Valcke, Christian

2006-01-01

We report carbon nanotube network field-effect transistors (NTNFETs) that function as selective detectors of DNA immobilization and hybridization. NTNFETs with immobilized synthetic oligonucleotides have been shown to specifically recognize target DNA sequences, including H63D single-nucleotide polymorphism (SNP) discrimination in the HFE gene, responsible for hereditary hemochromatosis. The electronic responses of NTNFETs upon single-stranded DNA immobilization and subsequent DNA hybridization events were confirmed by using fluorescence-labeled oligonucleotides and then were further explored for label-free DNA detection at picomolar to micromolar concentrations. We have also observed a strong effect of DNA counterions on the electronic response, thus suggesting a charge-based mechanism of DNA detection using NTNFET devices. Implementation of label-free electronic detection assays using NTNFETs constitutes an important step toward low-cost, low-complexity, highly sensitive and accurate molecular diagnostics. hemochromatosis | SNP | biosensor

Enhanced Stability of DNA Nanostructures by Incorporation of Unnatural Base Pairs.

PubMed

Liu, Qing; Liu, Guocheng; Wang, Ting; Fu, Jing; Li, Rujiao; Song, Linlin; Wang, Zhen-Gang; Ding, Baoquan; Chen, Fei

2017-11-03

Self-assembled DNA nanostructures hold great promise in the fields of nanofabrication, biosensing and nanomedicine. However, the inherent low stability of the DNA double helices, formed by weak interactions, largely hinders the assembly and functions of DNA nanostructures. In this study, we redesigned and constructed a six-arm DNA junction by incorporation of the unnatural base pairs 5-Me-isoC/isoG and A/2-thioT into the double helices. They not only retained the structural integrity of the DNA nanostructure, but also showed enhanced thermal stability and resistance to T7 Exonuclease digestion. This research may expand the applications of DNA nanostructures in nanofabrication and biomedical fields, and furthermore, the genetic alphabet expansion with unnatural base pairs may enable us to construct more complicated and diversified self-assembled DNA nanostructures. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

The future of forensic DNA analysis

PubMed Central

Butler, John M.

2015-01-01

The author's thoughts and opinions on where the field of forensic DNA testing is headed for the next decade are provided in the context of where the field has come over the past 30 years. Similar to the Olympic motto of ‘faster, higher, stronger’, forensic DNA protocols can be expected to become more rapid and sensitive and provide stronger investigative potential. New short tandem repeat (STR) loci have expanded the core set of genetic markers used for human identification in Europe and the USA. Rapid DNA testing is on the verge of enabling new applications. Next-generation sequencing has the potential to provide greater depth of coverage for information on STR alleles. Familial DNA searching has expanded capabilities of DNA databases in parts of the world where it is allowed. Challenges and opportunities that will impact the future of forensic DNA are explored including the need for education and training to improve interpretation of complex DNA profiles. PMID:26101278

Close encounters with DNA

PubMed Central

Maffeo, C.; Yoo, J.; Comer, J.; Wells, D. B.; Luan, B.; Aksimentiev, A.

2014-01-01

Over the past ten years, the all-atom molecular dynamics method has grown in the scale of both systems and processes amenable to it and in its ability to make quantitative predictions about the behavior of experimental systems. The field of computational DNA research is no exception, witnessing a dramatic increase in the size of systems simulated with atomic resolution, the duration of individual simulations and the realism of the simulation outcomes. In this topical review, we describe the hallmark physical properties of DNA from the perspective of all-atom simulations. We demonstrate the amazing ability of such simulations to reveal the microscopic physical origins of experimentally observed phenomena and we review the frustrating limitations associated with imperfections of present atomic force fields and inadequate sampling. The review is focused on the following four physical properties of DNA: effective electric charge, response to an external mechanical force, interaction with other DNA molecules and behavior in an external electric field. PMID:25238560

Relationship Between Frequency and Deflection Angle in the DNA Prism

PubMed Central

Chen, Zhen; Dorfman, Kevin D.

2013-01-01

The DNA prism is a modification of the standard pulsed-field electrophoresis protocol to provide a continuous separation, where the DNA are deflected at an angle that depends on their molecular weight. The standard switchback model for the DNA prism predicts a monotonic increase in the deflection angle as a function of the frequency for switching the field until a plateau regime is reached. However, experiments indicate that the deflection angle achieves a maximum value before decaying to a size-independent value at high frequencies. Using Brownian dynamics simulations, we show that the maximum in the deflection angle is related to the reorientation time for the DNA and the decay in deflection angle at high frequencies is due to inadequate stretching. The generic features of the dependence of the deflection angle on molecular weight, switching frequency, and electric field strength explain a number of experimental phenomena. PMID:23410375

Close encounters with DNA.

PubMed

Maffeo, C; Yoo, J; Comer, J; Wells, D B; Luan, B; Aksimentiev, A

2014-10-15

Over the past ten years, the all-atom molecular dynamics method has grown in the scale of both systems and processes amenable to it and in its ability to make quantitative predictions about the behavior of experimental systems. The field of computational DNA research is no exception, witnessing a dramatic increase in the size of systems simulated with atomic resolution, the duration of individual simulations and the realism of the simulation outcomes. In this topical review, we describe the hallmark physical properties of DNA from the perspective of all-atom simulations. We demonstrate the amazing ability of such simulations to reveal the microscopic physical origins of experimentally observed phenomena. We also discuss the frustrating limitations associated with imperfections of present atomic force fields and inadequate sampling. The review is focused on the following four physical properties of DNA: effective electric charge, response to an external mechanical force, interaction with other DNA molecules and behavior in an external electric field.

Manpower Management: No Tiered Readiness - Enabling The Nation’s Force in Readiness

DTIC Science & Technology

2013-04-09

physically qualified (medical, dental , panorex) 2,D, D,H,Q Pregnancy (after determination by proper authority) N Postpartum (up to six...identifying surfaces and gaps are elemental to both the Marine Corps Planning Process and this study, and assist in the overall problem framing. Manning...of the Marine Corps on T/Os. To assist the deputy commandants in this task, each occupational field retains an occupational field sponsor. The

STS-26 crewmembers walk across parking apron to T-38s at Ellington Field

NASA Technical Reports Server (NTRS)

1988-01-01

STS-26 Discovery, Orbiter Vehicle (OV) 103, crewmembers walk toward the flight line of T-38s at Ellington Field prior to their departure for Kennedy Space Center (KSC). STS-26 crewmembers are scheduled for a September 29 launch. Left to right are Mission Specialist (MS) David C. Hilmers, Pilot Richard O. Covey, Commander Frederick H. Hauck, MS George D. Nelson, and MS John M. Lounge.

Decentralizing Centralized Control: Reorienting a Fundamental Tenet for Resilient Air Operation

DTIC Science & Technology

2008-05-22

Forward Air Controller FBIS Foreign Broadcast Information Service FCC Functional Component Commander FM Field Manual GBU - 39 Guided Bomb Unit - 39...Diameter Bomb ( GBU - 39 ) TEG Test and Evaluation Group TST Time Sensitive Target UAV Unmanned Aerial Vehicle USAF United States Air Force USAFWS United...www.afmc.af.mil/news/story.asp?id=123017916 (accessed November 11, 2007). Recent fielding of the GBU - 39 , Small Diameter Bomb (SDB), enables a

KSC-03pd1103

NASA Image and Video Library

2003-04-10

KENNEDY SPACE CENTER, FLA. -- Kennedy Space Center workers chat with Forest Service workers at the Hemphill site in East Texas before heading out to the field for grid searches. KSC workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

KSC-03PD-1103

NASA Technical Reports Server (NTRS)

2003-01-01

KENNEDY SPACE CENTER, FLA. -- Kennedy Space Center workers chat with Forest Service workers at the Hemphill site in East Texas before heading out to the field for grid searches. KSC workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

Development of a molecular method for testing the effectiveness of UV systems on-site.

PubMed

Nizri, Limor; Vaizel-Ohayon, Dalit; Ben-Amram, Hila; Sharaby, Yehonatan; Halpern, Malka; Mamane, Hadas

2017-12-15

We established a molecular method for quantifying ultraviolet (UV) disinfection efficacy using total bacterial DNA in a water sample. To evaluate UV damage to the DNA, we developed the "DNA damage" factor, which is a novel cultivation-independent approach that reveals UV-exposure efficiency by applying a simple PCR amplification method. The study's goal was to prove the feasibility of this method for demonstrating the efficiency of UV systems in the field using flow-through UV reactors. In laboratory-based experiments using seeded bacteria, the DNA damage tests demonstrated a good correlation between PCR products and UV dose. In the field, natural groundwater sampled before and after being subjected to the full-scale UV reactors was filtered, and the DNA extracted from the filtrate was subjected to PCR amplification for a 900-bp fragment of the 16S rRNA gene with initial DNA concentrations of 0.1 and 1 ng/μL. In both cases, the UV dose predicted and explained a significant proportion of the variance in the log inactivation ratio and DNA damage factor. Log inactivation ratio was very low, as expected in groundwater due to low initial bacterial counts, whereas the DNA damage factor was within the range of values obtained in the laboratory-based experiments. Consequently, the DNA damage factor reflected the true performance of the full-scale UV system during operational water flow by using the indigenous bacterial array present in a water sample. By applying this method, we were able to predict with high confidence, the UV reactor inactivation potential. For method validation, laboratory and field iterations are required to create a practical field calibration curve that can be used to determine the expected efficiency of the full-scale UV system in the field under actual operation. Copyright © 2017 Elsevier Ltd. All rights reserved.

HmtDB 2016: data update, a better performing query system and human mitochondrial DNA haplogroup predictor

PubMed Central

Clima, Rosanna; Preste, Roberto; Calabrese, Claudia; Diroma, Maria Angela; Santorsola, Mariangela; Scioscia, Gaetano; Simone, Domenico; Shen, Lishuang; Gasparre, Giuseppe; Attimonelli, Marcella

2017-01-01

The HmtDB resource hosts a database of human mitochondrial genome sequences from individuals with healthy and disease phenotypes. The database is intended to support both population geneticists as well as clinicians undertaking the task to assess the pathogenicity of specific mtDNA mutations. The wide application of next-generation sequencing (NGS) has provided an enormous volume of high-resolution data at a low price, increasing the availability of human mitochondrial sequencing data, which called for a cogent and significant expansion of HmtDB data content that has more than tripled in the current release. We here describe additional novel features, including: (i) a complete, user-friendly restyling of the web interface, (ii) links to the command-line stand-alone and web versions of the MToolBox package, an up-to-date tool to reconstruct and analyze human mitochondrial DNA from NGS data and (iii) the implementation of the Reconstructed Sapiens Reference Sequence (RSRS) as mitochondrial reference sequence. The overall update renders HmtDB an even more handy and useful resource as it enables a more rapid data access, processing and analysis. HmtDB is accessible at http://www.hmtdb.uniba.it/. PMID:27899581

The development and validation of command schedules for SeaWiFS

NASA Astrophysics Data System (ADS)

Woodward, Robert H.; Gregg, Watson W.; Patt, Frederick S.

1994-11-01

An automated method for developing and assessing spacecraft and instrument command schedules is presented for the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) project. SeaWiFS is to be carried on the polar-orbiting SeaStar satellite in 1995. The primary goal of the SeaWiFS mission is to provide global ocean chlorophyll concentrations every four days by employing onboard recorders and a twice-a-day data downlink schedule. Global Area Coverage (GAC) data with about 4.5 km resolution will be used to produce the global coverage. Higher resolution (1.1 km resolution) Local Area Coverage (LAC) data will also be recorded to calibrate the sensor. In addition, LAC will be continuously transmitted from the satellite and received by High Resolution Picture Transmission (HRPT) stations. The methods used to generate commands for SeaWiFS employ numerous hierarchical checks as a means of maximizing coverage of the Earth's surface and fulfilling the LAC data requirements. The software code is modularized and written in Fortran with constructs to mirror the pre-defined mission rules. The overall method is specifically developed for low orbit Earth-observing satellites with finite onboard recording capabilities and regularly scheduled data downlinks. Two software packages using the Interactive Data Language (IDL) for graphically displaying and verifying the resultant command decisions are presented. Displays can be generated which show portions of the Earth viewed by the sensor and spacecraft sub-orbital locations during onboard calibration activities. An IDL-based interactive method of selecting and testing LAC targets and calibration activities for command generation is also discussed.

An electrooculogram-based binary saccade sequence classification (BSSC) technique for augmentative communication and control.

PubMed

Keegan, Johnalan; Burke, Edward; Condron, James

2009-01-01

In the field of assistive technology, the electrooculogram (EOG) can be used as a channel of communication and the basis of a man-machine interface. For many people with severe motor disabilities, simple actions such as changing the TV channel require assistance. This paper describes a method of detecting saccadic eye movements and the use of a saccade sequence classification algorithm to facilitate communication and control. Saccades are fast eye movements that occurs when a person's gaze jumps from one fixation point to another. The classification is based on pre-defined sequences of saccades, guided by a static visual template (e.g. a page or poster). The template, consisting of a table of symbols each having a clearly identifiable fixation point, is situated within view of the user. To execute a particular command, the user moves his or her gaze through a pre-defined path of eye movements. This results in a well-formed sequence of saccades which are translated into a command if a match is found in a library of predefined sequences. A coordinate transformation algorithm is applied to each candidate sequence of recorded saccades to mitigate the effect of changes in the user's position and orientation relative to the visual template. Upon recognition of a saccade sequence from the library, its associated command is executed. A preliminary experiment in which two subjects were instructed to perform a series of command sequences consisting of 8 different commands are presented in the final sections. The system is also shown to be extensible to facilitate convenient text entry via an alphabetic visual template.

DNA nanotechnology and its applications in biomedical research.

PubMed

Sun, Lifan; Yu, Lu; Shen, Wanqiu

2014-09-01

DNA nanotechnology, which uses DNA as a material to self-assemble designed nanostructures, including DNA 2D arrays, 3D nanostructures, DNA nanotubes and DNA nanomechanical devices, has showed great promise in biomedical applications. Various DNA nanostructures have been used for protein characterization, enzyme assembly, biosensing, drug delivery and biomimetic assemblies. In this review, we will present recent advances of DNA nanotechnology and its applications in biomedical research field.

Command Relationships of Active and National Guard Forces During Domestic Disaster Response in California

DTIC Science & Technology

2007-06-15

is replacing the term Military Assistance to Civil Authorities ( MACA ). Emergency Management. The provision of overall operational control or...both public and private, to organize field-level incident management operations. Military Assistance to Civil Authorities ( MACA ). The broad mission of

14. Photographic copy of construction drawing, dated May??, 19??, Department ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. Photographic copy of construction drawing, dated May??, 19??, Department of the Air Force, Air Defense Command, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. - Selfridge Field, Building No. 1050, Northwest corner of Doolittle Avenue & D Street; Harrison Township, Mount Clemens, Macomb County, MI

Embedded Neoliberalism within Faculty Behaviors

ERIC Educational Resources Information Center

Levin, John S.; Aliyeva, Aida

2015-01-01

Although there are claims that neoliberalism has not only commandeered the agenda and actions of universities and colleges but also become identified with the work of academic professionals, there is little empirical evidence to show that neoliberalism has infiltrated the work of faculty. This qualitative field work investigation of three…

Evaluation Theory, Models, and Applications

ERIC Educational Resources Information Center

Stufflebeam, Daniel L.; Shinkfield, Anthony J.

2007-01-01

"Evaluation Theory, Models, and Applications" is designed for evaluators and students who need to develop a commanding knowledge of the evaluation field: its history, theory and standards, models and approaches, procedures, and inclusion of personnel as well as program evaluation. This important book shows how to choose from a growing…

Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

PubMed

Williams, Maggie R; Stedtfeld, Robert D; Engle, Cathrine; Salach, Paul; Fakher, Umama; Stedtfeld, Tiffany; Dreelin, Erin; Stevenson, R Jan; Latimore, Jo; Hashsham, Syed A

2017-01-01

Loop-mediated isothermal amplification (LAMP) of aquatic invasive species environmental DNA (AIS eDNA) was used for rapid, sensitive, and specific detection of Dreissena sp. relevant to the Great Lakes (USA) basin. The method was validated for two uses including i) direct amplification of eDNA using a hand filtration system and ii) confirmation of the results after DNA extraction using a conventional thermal cycler run at isothermal temperatures. Direct amplification eliminated the need for DNA extraction and purification and allowed detection of target invasive species in grab or concentrated surface water samples, containing both free DNA as well as larger cells and particulates, such as veligers, eggs, or seeds. The direct amplification method validation was conducted using Dreissena polymorpha and Dreissena bugensis and uses up to 1 L grab water samples for high target abundance (e.g., greater than 10 veligers (larval mussels) per L for Dreissena sp.) or 20 L samples concentrated through 35 μm nylon screens for low target abundance, at less than 10 veligers per liter water. Surface water concentrate samples were collected over a period of three years, mostly from inland lakes in Michigan with the help of a network of volunteers. Field samples collected from 318 surface water locations included i) filtered concentrate for direct amplification validation and ii) 1 L grab water sample for eDNA extraction and confirmation. Though the extraction-based protocol was more sensitive (resulting in more positive detections than direct amplification), direct amplification could be used for rapid screening, allowing for quicker action times. For samples collected between May and August, results of eDNA direct amplification were consistent with known presence/absence of selected invasive species. A cross-platform smartphone application was also developed to disseminate the analyzed results to volunteers. Field tests of the direct amplification protocol using a portable device (Gene-Z) showed the method could be used in the field to obtain results within one hr (from sample to result). Overall, the direct amplification has the potential to simplify the eDNA-based monitoring of multiple aquatic invasive species. Additional studies are warranted to establish quantitative correlation between eDNA copy number, veliger, biomass or organismal abundance in the field.

Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

PubMed Central

Stedtfeld, Robert D.; Engle, Cathrine; Salach, Paul; Fakher, Umama; Stedtfeld, Tiffany; Dreelin, Erin; Stevenson, R. Jan; Latimore, Jo; Hashsham, Syed A.

2017-01-01

Loop-mediated isothermal amplification (LAMP) of aquatic invasive species environmental DNA (AIS eDNA) was used for rapid, sensitive, and specific detection of Dreissena sp. relevant to the Great Lakes (USA) basin. The method was validated for two uses including i) direct amplification of eDNA using a hand filtration system and ii) confirmation of the results after DNA extraction using a conventional thermal cycler run at isothermal temperatures. Direct amplification eliminated the need for DNA extraction and purification and allowed detection of target invasive species in grab or concentrated surface water samples, containing both free DNA as well as larger cells and particulates, such as veligers, eggs, or seeds. The direct amplification method validation was conducted using Dreissena polymorpha and Dreissena bugensis and uses up to 1 L grab water samples for high target abundance (e.g., greater than 10 veligers (larval mussels) per L for Dreissena sp.) or 20 L samples concentrated through 35 μm nylon screens for low target abundance, at less than 10 veligers per liter water. Surface water concentrate samples were collected over a period of three years, mostly from inland lakes in Michigan with the help of a network of volunteers. Field samples collected from 318 surface water locations included i) filtered concentrate for direct amplification validation and ii) 1 L grab water sample for eDNA extraction and confirmation. Though the extraction-based protocol was more sensitive (resulting in more positive detections than direct amplification), direct amplification could be used for rapid screening, allowing for quicker action times. For samples collected between May and August, results of eDNA direct amplification were consistent with known presence/absence of selected invasive species. A cross-platform smartphone application was also developed to disseminate the analyzed results to volunteers. Field tests of the direct amplification protocol using a portable device (Gene-Z) showed the method could be used in the field to obtain results within one hr (from sample to result). Overall, the direct amplification has the potential to simplify the eDNA-based monitoring of multiple aquatic invasive species. Additional studies are warranted to establish quantitative correlation between eDNA copy number, veliger, biomass or organismal abundance in the field. PMID:29036210

Magnetic studies of Co2+, Ni2+, and Zn2+-modified DNA double-crossover lattices

NASA Astrophysics Data System (ADS)

Dugasani, Sreekantha Reddy; Oh, Young Hoon; Gnapareddy, Bramaramba; Park, Tuson; Kang, Won Nam; Park, Sung Ha

2018-01-01

We fabricated divalent-metal-ion-modified DNA double-crossover (DX) lattices on a glass substrate and studied their magnetic characteristics as a function of ion concentrations [Co2+], [Ni2+] and [Zn2+]. Up to certain critical concentrations, the DNA DX lattices with ions revealed discrete S-shaped hysteresis, i.e. characteristics of strong ferromagnetism, with significant changes in the coercive field, remanent magnetization, and susceptibility. Induced magnetic dipoles formed by metal ions in DNA duplex in the presence of a magnetic field imparted ferromagnetic behaviour. By considering hysteresis and the magnitude of magnetization in a magnetization-magnetic field curve, Co2+-modified DNA DX lattices showed a relatively strong ferromagnetic nature with an increasing (decreasing) trend of coercive field and remanent magnetization when [Co2+] ≤ 1 mM ([Co2+] > 1 mM). In contrast, Ni2+ and Zn2+-modified DNA DX lattices exhibited strong and weak ferromagnetic behaviours at lower (≤1 mM for Ni2+ and ≤0.5 mM for Zn2+) and higher (>1 mM for Ni2+ and >0.5 mM for Zn2+) concentrations of ions, respectively. About 1 mM of [Co2+], [Ni2+] and [Zn2+] in DNA DX lattices was of special interest with regard to physical characteristics and was identified to be an optimum concentration of each ion. Finally, we measured the temperature-dependent magnetic characteristics of the metal-ion-modified DNA DX lattices. Nonzero magnetization and inverse susceptibility with almost constant values were observed between 25 and 300 K, with no indication of a magnetic transition. This indicated that the magnetic Curie temperatures of Co2+, Ni2+ and Zn2+-modified DNA DX lattices were above 300 K.

Eye-Pursuit and Reafferent Head Movement Signals Carried by Pursuit Neurons in the Caudal Part of the Frontal Eye Fields during Head-Free Pursuit

PubMed Central

Kasahara, Satoshi; Akao, Teppei; Kurkin, Sergei; Peterson, Barry W.

2009-01-01

Eye and head movements are coordinated during head-free pursuit. To examine whether pursuit neurons in frontal eye fields (FEF) carry gaze-pursuit commands that drive both eye-pursuit and head-pursuit, monkeys whose heads were free to rotate about a vertical axis were trained to pursue a juice feeder with their head and a target with their eyes. Initially the feeder and target moved synchronously with the same visual angle. FEF neurons responding to this gaze-pursuit were tested for eye-pursuit of target motion while the feeder was stationary and for head-pursuit while the target was stationary. The majority of pursuit neurons exhibited modulation during head-pursuit, but their preferred directions during eye-pursuit and head-pursuit were different. Although peak modulation occurred during head movements, the onset of discharge usually was not aligned with the head movement onset. The minority of neurons whose discharge onset was so aligned discharged after the head movement onset. These results do not support the idea that the head-pursuit–related modulation reflects head-pursuit commands. Furthermore, modulation similar to that during head-pursuit was obtained by passive head rotation on stationary trunk. Our results suggest that FEF pursuit neurons issue gaze or eye movement commands during gaze-pursuit and that the head-pursuit–related modulation primarily reflects reafferent signals resulting from head movements. PMID:18483002

The Aerosonde Robotic Aircraft: A New Paradigm for Environmental Observations.

NASA Astrophysics Data System (ADS)

Holland, G. J.; Webster, P. J.; Curry, J. A.; Tyrell, G.; Gauntlett, D.; Brett, G.; Becker, J.; Hoag, R.; Vaglienti, W.

2001-05-01

The Aerosonde is a small robotic aircraft designed for highly flexible and inexpensive operations. Missions are conducted in a completely robotic mode, with the aircraft under the command of a ground controller who monitors the mission. Here we provide an update on the Aerosonde development and operations and expand on the vision for the future, including instrument payloads, observational strategies, and platform capabilities. The aircraft was conceived in 1992 and developed to operational status in 1995-98, after a period of early prototyping. Continuing field operations and development since 1998 have led to the Aerosonde Mark 3, with ~2000 flight hours completed. A defined development path through to 2002 will enable the aircraft to become increasingly more robust with increased flexibility in the range and type of operations that can be achieved. An Aerosonde global reconnaissance facility is being developed that consists of launch and recovery sites dispersed around the globe. The use of satellite communications and internet technology enables an operation in which all aircraft around the globe are under the command of a single center. During operation, users will receive data at their home institution in near-real time via the virtual field environment, allowing the user to update the mission through interaction with the global command center. Sophisticated applications of the Aerosonde will be enabled by the development of a variety of interchangeable instrument payloads and the operation of Smart Aerosonde Clusters that allow a cluster of Aerosondes to interact intelligently in response to the data being collected.

Evolution and inheritance of animal mitochondrial DNA: rules and exceptions.

PubMed

Ladoukakis, Emmanuel D; Zouros, Eleftherios

2017-12-01

Mitochondrial DNA (mtDNA) has been studied intensely for "its own" merit. Its role for the function of the cell and the organism remains a fertile field, its origin and evolution is an indispensable part of the evolution of life and its interaction with the nuclear DNA is among the most important cases of genome synergism and co-evolution. Also, mtDNA was proven one of the most useful tools in population genetics and molecular phylogenetics. In this article we focus on animal mtDNA and discuss briefly how our views about its structure, function and transmission have changed, how these changes affect the information we have accumulated through its use in the fields of phylogeny and population structure and what are the most important questions that remain open for future research.

Experimental analysis of a TEM plane transmission line for DNA studies at 900 MHz EM fields

NASA Astrophysics Data System (ADS)

Belloni, F.; Doria, D.; Lorusso, A.; Nassisi, V.; Velardi, L.; Alifano, P.; Monaco, C.; Talà, A.; Tredici, M.; Rainò, A.

2006-07-01

A suitable plane transmission line was developed and its behaviour analysed at 900 MHz radiofrequency fields to study DNA mutability and the repair of micro-organisms. In this work, utilizing such a device, we investigated the behaviour of DNA mutability and repair of Escherichia coli strains. The transmission line was very simple and versatile in changing its characteristic resistance and field intensity by varying its sizes. In the absence of cell samples inside the transmission line, the relative modulation of the electric and/or magnetic field was ±31% with respect to the mean values, allowing the processing of more samples at different exposure fields in a single run. A slight decrease in spontaneous mutability to rifampicin-resistance of the E. coli JC411 strain was demonstrated in mismatch-repair proficient samples exposed to the radio-frequency fields during their growth on solid medium.

DNA Everywhere. A Guide for Simplified Environmental Genomic DNA Extraction Suitable for Use in Remote Areas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gabrielle N. Pecora; Francine C. Reid; Lauren M. Tom

2016-05-01

Collecting field samples from remote or geographically distant areas can be a financially and logistically challenging. With participation of a local organization where the samples are originated from, gDNA samples can be extracted from the field and shipped to a research institution for further processing and analysis. The ability to set up gDNA extraction capabilities in the field can drastically reduce cost and time when running long-term microbial studies with a large sample set. The method outlined here has developed a compact and affordable method for setting up a “laboratory” and extracting and shipping gDNA samples from anywhere in themore » world. This white paper explains the process of setting up the “laboratory”, choosing and training individuals with no prior scientific experience how to perform gDNA extractions and safe methods for shipping extracts to any research institution. All methods have been validated by the Andersen group at Lawrence Berkeley National Laboratory using the Berkeley Lab PhyloChip.« less

General Mission Analysis Tool (GMAT) User's Guide (Draft)

NASA Technical Reports Server (NTRS)

Hughes, Steven P.

2007-01-01

4The General Mission Analysis Tool (GMAT) is a space trajectory optimization and mission analysis system. This document is a draft of the users guide for the tool. Included in the guide is information about Configuring Objects/Resources, Object Fields: Quick Look-up Tables, and Commands and Events.

7. Photographic copy of floor plan drawing, dated August 10, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photographic copy of floor plan drawing, dated August 10, 1968, Department of the Air Force Air Defense Command Installations, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. - Selfridge Field, Building No. 592, South of East Joy Boulevard, west of Taxiway C, Mount Clemens, Macomb County, MI

6. Photographic copy of floor plan, dated August 10, 1968, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Photographic copy of floor plan, dated August 10, 1968, Department of the Air Force Air Defense Command Installation, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. - Selfridge Field, Building No. 1576, South of East Joy Boulevard, west of Taxiway C, Mount Clemens, Macomb County, MI

Photographic copy of floor plan drawing, dated August 10, 1968, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photographic copy of floor plan drawing, dated August 10, 1968, Department of the Air Force Air Defense Command Installations, in possession of Selfridge Base Museum, Mt. Clemens, Michigan - Selfridge Field, Building No. 1580, South of East Joy Boulevard, west of Taxiway C, Mount Clemens, Macomb County, MI

A Finite Deformation Analysis of the Near Field Surrounding the Tip of Crack Like Ellipses

DTIC Science & Technology

1974-05-01

Corporation ATTN: Mr. S. T. Rolfe , Applied Research Lab. Monroeville, Pa. 15146 University of Illinois ATTN: Mr. H..T. Corten, Department of...Research and Development Director U.S. Army Missile Command Huntsville, Alabama 1 Mr. John G. Merkle Building 9204-1 Oak Ridge National

4. Northeast corner of quarters (executive officer's quarters), looking onto ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Northeast corner of quarters (executive officer's quarters), looking onto Quarter R (commanding officer's quarters), looking southeast - Naval Air Station Chase Field, Texas State Highway 202, 4.8 miles east of intersection of Texas State Highway 202 & U.S. State Highway 181, Beeville, Bee County, TX

History, Philosophy, and Public Opinion Research.

ERIC Educational Resources Information Center

Herbst, Susan

1993-01-01

Argues for the importance of the classical tradition (broad, speculative, and historically informed writing and research) in public opinion research. Argues that asking large, normative questions about public opinion processes, trying to build grand theory, and taking history seriously will enrich the field and command the attention of scholars in…

46 CFR 46.10-60 - Control.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Control. 46.10-60 Section 46.10-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES SUBDIVISION LOAD LINES FOR PASSENGER VESSELS Administration § 46.10-60 Control. The Director, Field Operations (DFO) or the Coast Guard District Commander may...

46 CFR 46.10-60 - Control.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Control. 46.10-60 Section 46.10-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES SUBDIVISION LOAD LINES FOR PASSENGER VESSELS Administration § 46.10-60 Control. The Director, Field Operations (DFO) or the Coast Guard District Commander may...

46 CFR 46.10-60 - Control.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Control. 46.10-60 Section 46.10-60 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LOAD LINES SUBDIVISION LOAD LINES FOR PASSENGER VESSELS Administration § 46.10-60 Control. The Director, Field Operations (DFO) or the Coast Guard District Commander may...

A Management Information System for Bare Base Civil Engineering Commanders

DTIC Science & Technology

1988-09-01

initial beddown stage. The purpose of this research was to determine the feasibility of developing a microcomputer based management information system (MIS...the software best suited to synthesize four of the categories into a prototype field MIS. Keyword: Management information system , Bare bases, Civil engineering, Data bases, Information retrieval.

Apollo 11 Geology training

NASA Image and Video Library

1969-03-05

S69-25944 (25 Feb. 1969) --- These two Apollo 11 crew astronauts study rock samples during a geological field trip to the Quitman Mountains area near the Fort Quitman ruins in far west Texas. Neil A. Armstrong (in background) is the Apollo 11 commander; and Edwin E. Aldrin Jr. is the lunar module pilot.

Army Grit: Field Marshal Viscount Slims Key to Victory

DTIC Science & Technology

2017-05-25

provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently...in 1945, the Fourteenth Army and its commander, Field Marshal Viscount William Slim, demonstrated that they had the right stuff. To cope with war’s...demands, military personnel have to know what it means to dig deep during self-study, training , and execution. Psychologist and MacArthur “genius grant

Ethical Guidelines and Practices for US Military Medical Professionals

DTIC Science & Technology

2015-03-03

not been resolved through their chain of command. d) Develop a small cadre of clinicians with graduate level training in bioethics to serve as...Journal of Bioethics & Law. 2009;2(1). 7. Allhoff F. Physicians at War: The Dual-Loyalties Challenge. In: Allhoff F, ed. Physicians at War. Vol 41...ethics” has always been a field distinct from medical ethics, although related in many ways, and is a subset of the broader field of bioethics . It

Laboratory and Field Evaluation of Rapid Setting Cementitious Materials for Large Crater Repair

DTIC Science & Technology

2010-05-01

frame used within which to complete the repair was the current NATO standard of 4 hr. A total of 6 simulated craters were prepared, with each repair...Combat Command 129 Andrews Street Langley Air Force Base, VA 23665 ERDC TR-10-4 ii Abstract: Current practice for expedient runway repair...penalty. Numerous commercial products are available. A full-scale field test was conducted using rapid setting materials to repair simulated bomb craters

Army Logistician. Volume 38, Issue 3, May-June 2006

DTIC Science & Technology

2006-06-01

that are deployed or preparing to deploy and those in the process of transforming to a modular design find it difficult to keep abreast of emerging...The Air Force and the Marine Corps have well-defined and -established career fields in con- tracting for their NCOs. Now the Army is developing ...How will field -grade officers in a BCT be developed as logistics officers? How will CSS company commanders be developed ? Will there be a female

Simple method of DNA stretching on glass substrate for fluorescence image and spectroscopy

NASA Astrophysics Data System (ADS)

Neupane, Guru P.; Dhakal, Krishna P.; Lee, Hyunsoo; Guthold, Martin; Joseph, Vincent S.; Hong, Jong-Dal; Kim, Jeongyong

2013-05-01

Study of biological molecule DNA has contributed to developing many breaking thoughts and wide applications in multidisciplinary fields, such as genomic, medical, sensing and forensic fields. Stretching of DNA molecules is an important supportive tool for AFM or spectroscopic studies of DNA in a single molecular level. In this article, we established a simple method of DNA stretching (to its full length) that occurred on a rotating negatively-charged surface of glass substrate. The isolation of a single DNA molecule was attained by the two competitive forces on DNA molecules, that is, the electrostatic attraction developed between the positively charged YOYO-1 stained DNA and the negatively charged substrate, and the centrifugal force of the rotating substrate, which separates the DNA aggregates into the single molecule. Density of stretched DNA molecules was controlled by selecting the specific parameters such as spinning time and rates, loading volume of DNA-dye complex solution etc. The atomic force microscopy image exhibited a single DNA molecule on the negatively-charged substrate in an isolated state. Further, the photoluminescence spectra of a single DNA molecule stained with YOYO-1 were achieved using the method developed in the present study, which is strongly believed to effectively support the spectroscopic analysis of DNA in a single molecular level.

DNA methylation-based age prediction from various tissues and body fluids

PubMed Central

Jung, Sang-Eun; Shin, Kyoung-Jin; Lee, Hwan Young

2017-01-01

Aging is a natural and gradual process in human life. It is influenced by heredity, environment, lifestyle, and disease. DNA methylation varies with age, and the ability to predict the age of donor using DNA from evidence materials at a crime scene is of considerable value in forensic investigations. Recently, many studies have reported age prediction models based on DNA methylation from various tissues and body fluids. Those models seem to be very promising because of their high prediction accuracies. In this review, the changes of age-associated DNA methylation and the age prediction models for various tissues and body fluids were examined, and then the applicability of the DNA methylation-based age prediction method to the forensic investigations was discussed. This will improve the understandings about DNA methylation markers and their potential to be used as biomarkers in the forensic field, as well as the clinical field. PMID:28946940

Electromagnetic pulse (EMP), Part II: Field-expedient ways to minimize its effects on field medical treatment facilities.

PubMed

Vandre, R H; Klebers, J; Tesche, F M; Blanchard, J P

1993-05-01

Part I of this paper showed that a field commander can expect approximately 65% of his unprotected electronic medical equipment to be damaged by the electromagnetic pulse (EMP) from a single nuclear detonation as far as 2200 km away. Using computer modeling, field-expedient ways to minimize the effects of EMP were studied. The results were: (1) keep wiring near the ground, (2) keep wiring short, (3) unplug unused equipment, (4) run power cabling and tents in a magnetic north-south direction (avoid running power cabling in the east-west direction), and (5) place sensitive equipment in International Organization for Standardization shelters.

Self-assembly of multiferroic core-shell particulate nanocomposites through DNA-DNA hybridization and magnetic field directed assembly of superstructures

NASA Astrophysics Data System (ADS)

Sreenivasulu, Gollapudi; Lochbiler, Thomas A.; Panda, Manashi; Srinivasan, Gopalan; Chavez, Ferman A.

2016-04-01

Multiferroic composites of ferromagnetic and ferroelectric phases are of importance for studies on mechanical strain mediated coupling between the magnetic and electric subsystems. This work is on DNA-assisted self-assembly of superstructures of such composites with nanometer periodicity. The synthesis involved oligomeric DNA-functionalized ferroelectric and ferromagnetic nanoparticles, 600 nm BaTiO3 (BTO) and 200 nm NiFe2O4 (NFO), respectively. Mixing BTO and NFO particles, possessing complementary DNA sequences, resulted in the formation of ordered core-shell heteronanocomposites held together by DNA hybridization. The composites were imaged by scanning electron microscopy and scanning microwave microscopy. The presence of heteroassemblies along with core-shell architecture is clearly observed. The reversible nature of the DNA hybridization allows for restructuring the composites into mm-long linear chains and 2D-arrays in the presence of a static magnetic field and ring-like structures in a rotating-magnetic field. Strong magneto-electric (ME) coupling in as-assembled composites is evident from static magnetic field H induced polarization and low-frequency magnetoelectric voltage coefficient measurements. Upon annealing the nanocomposites at high temperatures, evidence for the formation of bulk composites with excellent cross-coupling between the electric and magnetic subsystems is obtained by H-induced polarization and low-frequency ME voltage coefficient. The ME coupling strength in the self-assembled composites is measured to be much stronger than in bulk composites with randomly distributed NFO and BTO prepared by direct mixing and sintering.

Application of the CometChip platform to assess DNA damage in field-collected blood samples from turtles.

PubMed

Sykora, Peter; Chiari, Ylenia; Heaton, Andrew; Moreno, Nickolas; Glaberman, Scott; Sobol, Robert W

2018-05-01

DNA damage has been linked to genomic instability and the progressive breakdown of cellular and organismal homeostasis, leading to the onset of disease and reduced longevity. Insults to DNA from endogenous sources include base deamination, base hydrolysis, base alkylation, and metabolism-induced oxidative damage that can lead to single-strand and double-strand DNA breaks. Alternatively, exposure to environmental pollutants, radiation or ultra-violet light, can also contribute to exogenously derived DNA damage. We previously validated a novel, high through-put approach to measure levels of DNA damage in cultured mammalian cells. This new CometChip Platform builds on the classical single cell gel electrophoresis or comet methodology used extensively in environmental toxicology and molecular biology. We asked whether the CometChip Platform could be used to measure DNA damage in samples derived from environmental field studies. To this end, we determined that nucleated erythrocytes from multiple species of turtle could be successfully evaluated in the CometChip Platform to quantify levels of DNA damage. In total, we compared levels of DNA damage in 40 animals from two species: the box turtle (Terrapene carolina) and the red-eared slider (Trachemys scripta elegans). Endogenous levels of DNA damage were identical between the two species, yet we did discover some sex-linked differences and changes in DNA damage accumulation. Based on these results, we confirm that the CometChip Platform allows for the measurement of DNA damage in a large number of samples quickly and accurately, and is particularly adaptable to environmental studies using field-collected samples. Environ. Mol. Mutagen. 59:322-333, 2018. © 2018 Wiley Periodicals, Inc. © 2018 Wiley Periodicals, Inc.

Analysis of DNA Double-Strand Breaks and Cytotoxicity after 7 Tesla Magnetic Resonance Imaging of Isolated Human Lymphocytes

PubMed Central

Guttek, Karina; Hartig, Roland; Godenschweger, Frank; Roggenbuck, Dirk; Ricke, Jens; Reinhold, Dirk; Speck, Oliver

2015-01-01

The global use of magnetic resonance imaging (MRI) is constantly growing and the field strengths increasing. Yet, only little data about harmful biological effects caused by MRI exposure are available and published research analyzing the impact of MRI on DNA integrity reported controversial results. This in vitro study aimed to investigate the genotoxic and cytotoxic potential of 7 T ultra-high-field MRI on isolated human peripheral blood mononuclear cells. Hence, unstimulated mononuclear blood cells were exposed to 7 T static magnetic field alone or in combination with maximum permissible imaging gradients and radiofrequency pulses as well as to ionizing radiation during computed tomography and γ-ray exposure. DNA double-strand breaks were quantified by flow cytometry and automated microscopy analysis of immunofluorescence stained γH2AX. Cytotoxicity was studied by CellTiter-Blue viability assay and [3H]-thymidine proliferation assay. Exposure of unstimulated mononuclear blood cells to 7 T static magnetic field alone or combined with varying gradient magnetic fields and pulsed radiofrequency fields did not induce DNA double-strand breaks, whereas irradiation with X- and γ-rays led to a dose-dependent induction of γH2AX foci. The viability assay revealed a time- and dose-dependent decrease in metabolic activity only among samples exposed to γ-radiation. Further, there was no evidence for altered proliferation response after cells were exposed to 7 T MRI or low doses of ionizing radiation (≤ 0.2 Gy). These findings confirm the acceptance of MRI as a safe non-invasive diagnostic imaging tool, but whether MRI can induce other types of DNA lesions or DNA double-strand breaks during altered conditions still needs to be investigated. PMID:26176601

Threading dynamics of a polymer through parallel pores: Potential applications to DNA size separation

NASA Astrophysics Data System (ADS)

Åkerman, Björn

1997-04-01

DNA orientation measurements by linear dichroism (LD) spectroscopy and single molecule imaging by fluorescence microscopy are used to investigate the effect of DNA size (71-740 kilo base pairs) and field strength E (1-5.9 V/cm) on the conformation dynamics during the field-driven threading of DNA molecules through a set of parallel pores in agarose gels, with average pore radii between 380 Å and 1400 Å. Locally relaxed but globally oriented DNA molecules are subjected to a perpendicular field, and the observed LD time profile is compared with a recent theory for the threading [D. Long and J.-L. Viovy, Phys. Rev. E 53, 803 (1996)] which assumes the same initial state. As predicted the DNA is driven by the ends into a U-form, leading to an overshoot in the LD. The overshoot-time scales as E-(1.2-1.4) as predicted, but grows more slowly with DNA size than the predicted linear dependence. For long molecules loops form initially in the threading process but are finally consumed by the ends, and the process of transfer of DNA segments, from the loops to the arms of the U, leads to a shoulder in the LD as predicted. The critical size below which loops do not form (as indicated by the LD shoulder being absent) is between 71 and 105 kbp (0.5% agarose, 5.9 V/cm), and considerably larger than predicted because in the initial state the DNA molecules are housed in gel cavities with effective pore sizes about four times larger than the average pore size. From the data, the separation of DNA by exploiting the threading dynamics in pulsed fields [D. Long et al., CR Acad. Sci. Paris, Ser. IIb 321, 239 (1995)] is shown to be feasible in principle in an agarose-based system.

The future of forensic DNA analysis.

PubMed

Butler, John M

2015-08-05

The author's thoughts and opinions on where the field of forensic DNA testing is headed for the next decade are provided in the context of where the field has come over the past 30 years. Similar to the Olympic motto of 'faster, higher, stronger', forensic DNA protocols can be expected to become more rapid and sensitive and provide stronger investigative potential. New short tandem repeat (STR) loci have expanded the core set of genetic markers used for human identification in Europe and the USA. Rapid DNA testing is on the verge of enabling new applications. Next-generation sequencing has the potential to provide greater depth of coverage for information on STR alleles. Familial DNA searching has expanded capabilities of DNA databases in parts of the world where it is allowed. Challenges and opportunities that will impact the future of forensic DNA are explored including the need for education and training to improve interpretation of complex DNA profiles. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

Optical Voltage Sensing Using DNA Origami

PubMed Central

2018-01-01

We explore the potential of DNA nanotechnology for developing novel optical voltage sensing nanodevices that convert a local change of electric potential into optical signals. As a proof-of-concept of the sensing mechanism, we assembled voltage responsive DNA origami structures labeled with a single pair of FRET dyes. The DNA structures were reversibly immobilized on a nanocapillary tip and underwent controlled structural changes upon application of an electric field. The applied field was monitored through a change in FRET efficiency. By exchanging the position of a single dye, we could tune the voltage sensitivity of our DNA origami structure, demonstrating the flexibility and versatility of our approach. The experimental studies were complemented by coarse-grained simulations that characterized voltage-dependent elastic deformation of the DNA nanostructures and the associated change in the distance between the FRET pair. Our work opens a novel pathway for determining the mechanical properties of DNA origami structures and highlights potential applications of dynamic DNA nanostructures as voltage sensors. PMID:29430924

[Statistical analysis using freely-available "EZR (Easy R)" software].

PubMed

Kanda, Yoshinobu

2015-10-01

Clinicians must often perform statistical analyses for purposes such evaluating preexisting evidence and designing or executing clinical studies. R is a free software environment for statistical computing. R supports many statistical analysis functions, but does not incorporate a statistical graphical user interface (GUI). The R commander provides an easy-to-use basic-statistics GUI for R. However, the statistical function of the R commander is limited, especially in the field of biostatistics. Therefore, the author added several important statistical functions to the R commander and named it "EZR (Easy R)", which is now being distributed on the following website: http://www.jichi.ac.jp/saitama-sct/. EZR allows the application of statistical functions that are frequently used in clinical studies, such as survival analyses, including competing risk analyses and the use of time-dependent covariates and so on, by point-and-click access. In addition, by saving the script automatically created by EZR, users can learn R script writing, maintain the traceability of the analysis, and assure that the statistical process is overseen by a supervisor.

INFLIGHT - APOLLO XVI (CREW)

NASA Image and Video Library

1972-04-07

S72-35971 (21 April 1972) --- A 360-degree field of view of the Apollo 16 Descartes landing site area composed of individual scenes taken from color transmission made by the color RCA TV camera mounted on the Lunar Roving Vehicle (LRV). This panorama was made while the LRV was parked at the rim of North Ray Crater (Stations 11 & 12) during the third Apollo 16 lunar surface extravehicular activity (EVA) by astronauts John W. Young and Charles M. Duke Jr. The overlay identifies the directions and the key lunar terrain features. The camera panned across the rear portion of the LRV in its 360-degree sweep. Note Young and Duke walking along the edge of the crater in one of the scenes. The TV camera was remotely controlled from a console in the Mission Control Center (MCC). Astronauts Young, commander; and Duke, lunar module pilot; descended in the Apollo 16 Lunar Module (LM) "Orion" to explore the Descartes highlands landing site on the moon. Astronaut Thomas K. Mattingly II, command module pilot, remained with the Command and Service Modules (CSM) "Casper" in lunar orbit.

STS-93 / Columbia Flight Crew Photo Op & QA at Pad for TCDT

NASA Technical Reports Server (NTRS)

1999-01-01

The primary objective of the STS-93 mission was to deploy the Advanced X-ray Astrophysical Facility, which had been renamed the Chandra X-ray Observatory in honor of the late Indian-American Nobel Laureate Subrahmanyan Chandrasekhar. The mission was launched at 12:31 on July 23, 1999 onboard the space shuttle Columbia. The mission was led by Commander Eileen Collins. The crew was Pilot Jeff Ashby and Mission Specialists Cady Coleman, Steve Hawley and Michel Tognini from the Centre National d'Etudes Spatiales (CNES). This videotape shows a pre-flight press conference. Prior to the astronauts' arrival at the bunker area in front of the launch pad, the narrator discusses some of the training that the astronauts are scheduled to have prior to the launch, particularly the emergency egress procedures. Commander Collins introduces the crew and fields questions from the assembled press. Many questions are asked about the experiences of Commander Collins, and Mission Specialist Coleman as women in NASA. The press conference takes place outside in front of the Shuttle Columbia on the launch pad.

SCORPION persistent surveillance system with universal gateway

NASA Astrophysics Data System (ADS)

Coster, Michael; Chambers, Jon; Winters, Michael; Belesi, Joe

2008-04-01

This paper addresses benefits derived from the universal gateway utilized in Northrop Grumman Systems Corporation's (NGSC) SCORPION, a persistent surveillance and target recognition system produced by the Xetron campus in Cincinnati, Ohio. SCORPION is currently deployed in Operations Iraqi Freedom (OIF) and Enduring Freedom (OEF). The SCORPION universal gateway is a flexible, field programmable system that provides integration of over forty Unattended Ground Sensor (UGS) types from a variety of manufacturers, multiple visible and thermal electro-optical (EO) imagers, and numerous long haul satellite and terrestrial communications links, including the Army Research Lab (ARL) Blue Radio. Xetron has been integrating best in class sensors with this universal gateway to provide encrypted data exfiltration and remote sensor command and control since 1998. SCORPION data can be distributed point to point, or to multiple Common Operational Picture (COP) systems, including Command and Control Personal Computer (C2PC), Common Data Interchange Format for the Situational Awareness Display (CDIF/SAD), Force XXI Battle Command Brigade and Below (FBCB2), Defense Common Ground Systems (DCGS), and Remote Automated Position Identification System (RAPIDS).

STS-81 Flight Day 8

NASA Technical Reports Server (NTRS)

1997-01-01

On this eighth day of the STS-81 mission, the flight crew, Cmdr. Michael A. Baker, Pilot Brent W. Jett, Mission Specialists, John M. Grunsfeld, Marsha S. Ivins, Peter J.K. Wisoff, and John Blaha, bid farewell to Jerry Linenger and cosmonauts of Mir. Prior to hatch closure, the astronauts and cosmonauts conduct a formal farewell ceremony in the Mir Core Module. They then field questions from Russian and U.S. reporters in a joint news conference. Commander Mike Baker, Pilot Brent Jett and Mission Specialists Jeff Wisoff, John Grunsfeld, Marsha Ivins and John Blaha say goodbye to Mir 22 Commander Valery Korzun, Flight Engineer Alexander Kaleri and the newest Mir crewmember, astronaut Jerry Linenger. The hatches on the two spacecraft are closed.

Effects of Command and Control Vehicle (C2V) operational environment on soldier health and performance

NASA Technical Reports Server (NTRS)

Cowings, P. S.; Toscano, W. B.; DeRoshia, C.; Tauso, R.

2001-01-01

The purpose of this project was to use NASA technology to assist the US Army in the assessment of motion sickness incidences and effects on soldier performance and mood states within the Command and Control Vehicle (C2V). Specific objectives were (1) to determine if there was a significant difference between three internal configurations of the C2V and/or between seats within these vehicles; (2) to determine if there was a significant difference between the park, move, or short-halt field conditions; and (3) to validate a method of converging indicators developed by NASA to assess environmental impact of long duration spaceflight on crewmembers, using a large sample of subjects under ground-based operational conditions.

A spaceborne receiver for measuring electromagnetic field intensity

NASA Technical Reports Server (NTRS)

Reich, B. W.; Van Dusen, M. R.; Habib, E. J.

1973-01-01

Description of a very accurately controlled receiver for monitoring the electromagnetic radiations in both existing and projected space communication bands. Based on analysis of the existing and projected space communication bands, 108 to 174 MHz, 240 to 478 MHz, and 1535 to 1665 MHz were covered. The receiver achieves accurate control via a digitally tuned synthesizer and a wide range of digital control including frequency band coverage and gain control selection. Digital memory was provided to store 16 separate digital command instructions which can be programmed via a command data link. The receiver provides for transmission to the ground of both a predetection signal and signals in digital format, which in turn, were provided by sampling and analog-to-digital conversions.

High mobility organic field-effect transistor based on water-soluble deoxyribonucleic acid via spray coating

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shi, Wei; Han, Shijiao; Huang, Wei

High mobility organic field-effect transistors (OFETs) by inserting water-soluble deoxyribonucleic acid (DNA) buffer layer between electrodes and pentacene film through spray coating process were fabricated. Compared with the OFETs incorporated with DNA in the conventional organic solvents of ethanol and methanol: water mixture, the water-soluble DNA based OFET exhibited an over four folds enhancement of field-effect mobility from 0.035 to 0.153 cm{sup 2}/Vs. By characterizing the surface morphology and the crystalline structure of pentacene active layer through atomic force microscope and X-ray diffraction, it was found that the adoption of water solvent in DNA solution, which played a key role inmore » enhancing the field-effect mobility, was ascribed to both the elimination of the irreversible organic solvent-induced bulk-like phase transition of pentacene film and the diminution of a majority of charge trapping at interfaces in OFETs.« less

The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol–chloroform–isoamyl alcohol DNA extraction

PubMed Central

Renshaw, Mark A; Olds, Brett P; Jerde, Christopher L; McVeigh, Margaret M; Lodge, David M

2015-01-01

Current research targeting filtered macrobial environmental DNA (eDNA) often relies upon cold ambient temperatures at various stages, including the transport of water samples from the field to the laboratory and the storage of water and/or filtered samples in the laboratory. This poses practical limitations for field collections in locations where refrigeration and frozen storage is difficult or where samples must be transported long distances for further processing and screening. This study demonstrates the successful preservation of eDNA at room temperature (20 °C) in two lysis buffers, CTAB and Longmire's, over a 2-week period of time. Moreover, the preserved eDNA samples were seamlessly integrated into a phenol–chloroform–isoamyl alcohol (PCI) DNA extraction protocol. The successful application of the eDNA extraction to multiple filter membrane types suggests the methods evaluated here may be broadly applied in future eDNA research. Our results also suggest that for many kinds of studies recently reported on macrobial eDNA, detection probabilities could have been increased, and at a lower cost, by utilizing the Longmire's preservation buffer with a PCI DNA extraction. PMID:24834966

Axe: rapid, competitive sequence read demultiplexing using a trie.

PubMed

Murray, Kevin D; Borevitz, Justin O

2018-06-01

We describe a rapid algorithm for demultiplexing DNA sequence reads with in-read indices. Axe selects the optimal index present in a sequence read, even in the presence of sequencing errors. The algorithm is able to handle combinatorial indexing, indices of differing length, and several mismatches per index sequence. Axe is implemented in C, and is used as a command-line program on Unix-like systems. Axe is available online at https://github.com/kdmurray91/axe, and is available in Debian/Ubuntu distributions of GNU/Linux as the package axe-demultiplexer. Kevin Murray axe@kdmurray.id.au. Supplementary data are available at Bioinformatics online.

Mitochondrial DNA and Cancer Epidemiology Workshop

Cancer.gov

A workshop to review the state-of-the science in the mitochondrial DNA field and its use in cancer epidemiology, and to develop a concept for a research initiative on mitochondrial DNA and cancer epidemiology.

Protocol for collecting eDNA samples from streams [Version 2.3

Treesearch

K. J. Carim; T. Wilcox; M. K. Young; K. S. McKelvey; M. K. Schwartz

2015-01-01

Throughout the 2014 field season, we had over two dozen biologist throughout the western US collect over 300 samples for eDNA analysis with paired controls. Control samples were collected by filtering 0.5 L of distilled water. No samples had any evidence of field contamination. This method of sampling verifies the cleanliness of the field equipment, as well as the...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Physical device safety is typically implemented locally using embedded controllers, while operations safety is primarily performed in control centers. Safe operations can be enhanced by correct design of device-level control algorithms, and protocols, procedures and operator training at the control-room level, but all can fail. Moreover, these elements exchange data and issue commands via vulnerable communication layers. In order to secure these gaps and enhance operational safety, we believe monitoring of command sequences must be combined with an awareness of physical device limitations and automata models that capture safety mechanisms. One way of doing this is by leveraging specification-based intrusionmore » detection to monitor for physical constraint violations. The method can also verify that physical infrastructure state is consistent with monitoring information and control commands exchanged between field devices and control centers. This additional security layer enhances protection from both outsider attacks and insider mistakes. We implemented specification-based SCADA command analyzers using physical constraint algorithms directly in the Bro framework and Broccoli APIs for three separate scenarios: a water heater, an automated distribution system, and an over-current protection scheme. To accomplish this, we added low-level analyzers capable of examining control system-specific protocol packets for both Modbus TCP and DNP3, and also higher-level analyzers able to interpret device command and data streams within the context of each device's physical capabilities and present operational state. Thus the software that we are making available includes the Bro/Broccoli scripts for these three scenarios, as well as simulators, written in C, of those scenarios that generate sample traffic that is monitored by the Bro/Broccoli scripts. In addition, we have also implemented systems to directly pull cyber-physical information from the OSIsoft PI historian system. We have included the Python scripts used to perform that monitoring.« less

[The influence of electromagnetic fields on flora and fauna].

PubMed

Rochalska, Małgorzata

2009-01-01

This paper presents the influence of natural and artificial electromagnetic fields (EMF) on fauna and flora. The mechanisms of Earth's magnetic field detection and the use of this skill by migratory animals to faultlessly reach the destination of their travel are discussed, as well as the positive effects of electric and magnetic fields on plants relative to their physiology, yielding and health. EMF influence on social insects and animal organisms, including possible DNA damages and DNA repair systems, is presented. The influence of high frequency electromagnetic fields on birds nesting is also discussed.

Time to Spread Your Wings: A Review of the Avian Ancient DNA Field

PubMed Central

Grealy, Alicia; Rawlence, Nicolas J.

2017-01-01

Ancient DNA (aDNA) has the ability to inform the evolutionary history of both extant and extinct taxa; however, the use of aDNA in the study of avian evolution is lacking in comparison to other vertebrates, despite birds being one of the most species-rich vertebrate classes. Here, we review the field of “avian ancient DNA” by summarising the past three decades of literature on this topic. Most studies over this time have used avian aDNA to reconstruct phylogenetic relationships and clarify taxonomy based on the sequencing of a few mitochondrial loci, but recent studies are moving toward using a comparative genomics approach to address developmental and functional questions. Applying aDNA analysis with more practical outcomes in mind (such as managing conservation) is another increasingly popular trend among studies that utilise avian aDNA, but the majority of these have yet to influence management policy. We find that while there have been advances in extracting aDNA from a variety of avian substrates including eggshell, feathers, and coprolites, there is a bias in the temporal focus; the majority of the ca. 150 studies reviewed here obtained aDNA from late Holocene (100–1000 yBP) material, with few studies investigating Pleistocene-aged material. In addition, we identify and discuss several other issues within the field that require future attention. With more than one quarter of Holocene bird extinctions occurring in the last several hundred years, it is more important than ever to understand the mechanisms driving the evolution and extinction of bird species through the use of aDNA. PMID:28718817

KSC-03pd1113

NASA Image and Video Library

2003-04-09

KENNEDY SPACE CENTER, FLA. -- Kennedy Space Center and Forest Service workers pose in front of a helicopter landed at the Nacogdoches field site. The helicopter is one of those being used to survey for parts of Columbia. KSC workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

Real-Time DNA Sequencing in the Antarctic Dry Valleys Using the Oxford Nanopore Sequencer

PubMed Central

Johnson, Sarah S.; Zaikova, Elena; Goerlitz, David S.; Bai, Yu; Tighe, Scott W.

2017-01-01

The ability to sequence DNA outside of the laboratory setting has enabled novel research questions to be addressed in the field in diverse areas, ranging from environmental microbiology to viral epidemics. Here, we demonstrate the application of offline DNA sequencing of environmental samples using a hand-held nanopore sequencer in a remote field location: the McMurdo Dry Valleys, Antarctica. Sequencing was performed using a MK1B MinION sequencer from Oxford Nanopore Technologies (ONT; Oxford, United Kingdom) that was equipped with software to operate without internet connectivity. One-direction (1D) genomic libraries were prepared using portable field techniques on DNA isolated from desiccated microbial mats. By adequately insulating the sequencer and laptop, it was possible to run the sequencing protocol for up to 2½ h under arduous conditions. PMID:28337073

Wide Field Spectroscopy of Diffusing and Interacting DNA Using Tunable Nanoscale Geometries

NASA Astrophysics Data System (ADS)

Scott, Shane; Leith, Jason; Brandao, Hugo; Sehayek, Simon; Hofkirchner, Alexander; Laurin, Jill; Berard, Daniel; Verge, Alexander; Wiseman, Paul; Leslie, Sabrina

2015-03-01

It remains an outstanding challenge to directly image interacting and diffusing biomolecules under physiological conditions. Many biochemical questions can be posed in the form: Does A interact with B? What are the energetics, kinetics, stoichiometry, and cooperativity of this interaction? To tackle this challenge, we use tunable nanoscale confinement to perform wide-field imaging of interacting DNA molecules in free solution, under an extended range of reagent concentrations and interaction rates. We present the integration of ``Convex Lens-induced Confinement (CLiC)'' microscopy with image correlation analysis, simultaneously suppressing background fluorescence and extending imaging times. The measured DNA-DNA interactions would be inaccessible to standard techniques but are important for developing a mechanistic understanding of life-preserving processes such as DNA transcription. NSERC.

Moving environmental DNA methods from concept to practice for monitoring aquatic macroorganisms

USGS Publications Warehouse

Goldberg, Caren S.; Strickler, Katherine M.; Pilliod, David S.

2015-01-01

The discovery that macroorganisms can be detected from their environmental DNA (eDNA) in aquatic systems has immense potential for the conservation of biological diversity. This special issue contains 11 papers that review and advance the field of eDNA detection of vertebrates and other macroorganisms, including studies of eDNA production, transport, and degradation; sample collection and processing to maximize detection rates; and applications of eDNA for conservation using citizen scientists. This body of work is an important contribution to the ongoing efforts to take eDNA detection of macroorganisms from technical breakthrough to established, reliable method that can be used in survey, monitoring, and research applications worldwide. While the rapid advances in this field are remarkable, important challenges remain, including consensus on best practices for collection and analysis, understanding of eDNA diffusion and transport, and avoidance of inhibition in sample collection and processing. Nonetheless, as demonstrated in this special issue, eDNA techniques for research and monitoring are beginning to realize their potential for contributing to the conservation of biodiversity globally.

Implementation of Novel Design Features for qPCR-Based eDNA Assessment

PubMed Central

Veldhoen, Nik; Hobbs, Jared; Ikonomou, Georgios; Hii, Michael; Lesperance, Mary; Helbing, Caren C.

2016-01-01

Environmental stewardship requires timely, accurate information related to the status of a given ecosystem and the species that occupy it. Recent advances in the application of the highly sensitive real-time quantitative polymerase chain reaction (qPCR) towards identification of constituents within environmental DNA (eDNA) now allow targeted detection of the presence of species-specific biological material within a localized geographic region. However, as with all molecular techniques predicated on the specificity and sensitivity of the PCR assay, careful validation of each eDNA qPCR assay in development must be performed both under controlled laboratory conditions and when challenged with field-derived eDNA samples. Such a step-wise approach forms the basis for incorporation of innovative qPCR design features that strengthen the implementation and interpretation of the eDNA assay. This includes empirical determination that the qPCR assay is refractory to the presence of human DNA and the use of a tripartite assay approach comprised of 1) a primer set targeting plant chloroplast that evaluates the presence of amplifiable DNA from field samples to increase confidence in a negative result, 2) an animal group primer set to increase confidence in the assay result, and 3) a species-specific primer set to assess presence of DNA from the target species. To demonstrate this methodology, we generated eDNA assays specific for the North American bullfrog (Lithobates (Rana) catesbeiana) and the Rocky Mountain tailed frog (Ascaphus montanus) and characterized each with respect to detection sensitivity and specificity with demonstrated performance in a field survey scenario. The qPCR design features presented herein address specific challenges of eDNA assays thereby increasing their interpretative power. PMID:27802293

Comparison of Job Attitudes between Physicians, Nurses, Other Medical Officers, and other Air Force Officers.

DTIC Science & Technology

1986-04-01

was to satisfy the author’s curiosity about the job attitudes of physicians and nurses in the Air Force medical career field. There are very few...feedback to leaders and managers within the medical career field. The fourth was to fulfill a requirement for graduation from Air Command and Staff College...version of the style prescribed by the American Psychological Association. I am indebted to many individuals who provided support in the completion of

The King is Dead: Regaining the Throne -- The Current State of the Field Artillery, Core Competency Atrophy, and the Way Ahead

DTIC Science & Technology

2011-03-17

with a built -in glass ceiling is not likely to retain or attract the best and the brightest. If it hasn’t happened already, FA accessions will begin...officer. This would also facilitate removing the glass ceiling currently atop the field 19 artillery with only six tactical command opportunities. This...missions excluding conventional warfare strategic failure can result. 71 In summer 2006 in southern Lebanon , the Israeli army suffered a significant

Engineer Company Force Structure Force Modularization in Support of Decisive Action. Does the Corps of Engineers Need to Re-Structure Engineer Construction Companies Again in order to Support Decisive Actions?

DTIC Science & Technology

2012-05-16

Regional Command RCP Route Clearance Platoon RSOI Reception, Staging, Onward Movement, Integration SBCT Stryker Brigade Combat Team TOE Table of...Point (ASPs), and field hospital platforms; prepare river crossing sites; and support port repair due to Hydraulic Excavator (HYEX), provides force...platforms, FARPS, supply routes, roads, control points, fire bases, tank ditches, ASPs, and field hospital platforms; prepare river crossing sites; and

Special Operations Forces (SOF): An Integral Part of the Theater Operating System

DTIC Science & Technology

1994-02-08

Special Operations Forces (SOF) Operati’c-al MOUC Theater Operating Syst,:- Opelrational Fires G-eration Desert Sh.eld/;e sert Stormi Ccc.Tnd & cnrtrcl~ It...INTRODUCTION The classroom at the College of Naval Command & Staff was filled with field grade Army officers. The day’s lesson, part of the Army’s...service component SOF units; readers are inviter’ to turn to the many field manuals and publications which describe the characteristics of special

Executive Report: JSOU (Joint Special Operations University) First Annual Symposium, 2-5 May 2006, Hurlburt Field, Florida

DTIC Science & Technology

2006-05-05

NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Joint Special Operations University,357 Tully Street...Alison Building,Hurlburt Field,FL,32544 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR...leadership. JSOU is a subordinate organization of the US Special Operations Command (USSOCOM), MacDill Air Force Base, Florida. The mission of the Joint

The Combat Vehicle Command and Control System. Combat Performance of Armor Battalions Using Distributed Interactive Simulation

DTIC Science & Technology

1993-09-16

research staff members manned the TOC, and assumed key roles in the battalion staff. TOC staff members were selected for their extensive military ...CRA&I DTIC TAB Unannounced 0 Justification SBy - Distibution I Availability Codes Avail and / or Dist SpecialI 1 Field Unit at Fort Knox, Kentucky...Project Number Training Simulation 3 Approved for public release; distribution is unlimited. I iii I I I FOREWORD The Fort Knox Field Unit of the U.S

In vivo conformation of mitochondrial DNA revealed by pulsed-field gel electrophoresis in the true slime mold, Physarum polycephalum.

PubMed

Sakurai, R; Sasaki, N; Takano, H; Abe, T; Kawano, S

2000-04-28

Pulsed-field gel electrophoresis (PFGE) was used to examine the in vivo and in vitro conformations of Physarum polycephalum mitochondrial DNA (mtDNA). We used plugs containing isolated mitochondria, isolated mitochondrial nucleoids (mt-nuclei), and isolated mtDNA, in addition to whole cells. The mtDNA contained in the myxamoebae, plasmodia, isolated mitochondria, and isolated mt-nuclei was circular, but most of the isolated mtDNA had been site-specifically fragmented and linearized during DNA preparation and storage under low ionic strength conditions. Restriction mapping of Physarum mtDNA by the direct digestion of the isolated mt-nuclei from two different strains, DP89 x AI16 and KM88 x AI16, resulted in the circular form. A linear mitochondrial plasmid, mF, is known to promote mitochondrial fusion and integration of itself into the mtDNA in Physarum. Linearization of mtDNA by the integration of the mF plasmid was demonstrated when we used PFGE to analyze isolated mitochondria from the plasmodial strain DP89 x NG7 carrying the mF plasmid (mF+). The PFGE system can be used not only to determine whether the form of mtDNA is linear or circular but also to analyze the dynamic conformational changes of mtDNA.

Interagency Evaluation of the Section 1206 Global Train and Equip Program

DTIC Science & Technology

2009-08-31

Capabilities, Joint Staff, U.S. Africa Command, U.S. Central Command, U.S Joint Forces Command, U.S. Pacific Command, U.S. Southern Command, U.S. Special...Intensity Conflict & Interdependent Capabilities; Commanders of U.S. Africa Command, U.S. Central Command, U.S. Joint Forces Command, U.S. Pacific... Central Command, commented that coordinating the Section 1206 project proposal with the partner nation prior to submission would inflate the

Charting the Impacts of University-Child Welfare Collaboration.

ERIC Educational Resources Information Center

Briar-Lawson, Katharine, Ed.; Zlotnik, Joan Levy, Ed.

Few fields of service command as much public attention as child welfare. Workforce development is often a less visible yet critical response to service delivery crises and efforts to improve child and family outcomes. In the late 1980s, a campaign was undertaken to rebuild public child welfare systems with trained social workers. The subsequent…

Photographic copy of construction drawing, dated October 19, 1962, Department ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photographic copy of construction drawing, dated October 19, 1962, Department of the Air Force Air Defense Command Installations, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. ALTER POL OPERATIONS, SHEET 1 OF 2, SLF 140-070 - Selfridge Field, Building No. 1445, Sugarbush Road north of Bunker Road, Mount Clemens, Macomb County, MI

9. Photographic copy of first and second floor plans, undated, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Photographic copy of first and second floor plans, undated, Department of the Air Force, Air Defense Command Installations, Washington, D.C., in possession of Selfridge Base Museum, Mt. Clemens, Michigan. FLOOR PLANS. - Selfridge Field, Building No. 509, Sugarbush Road east of West Perimeter Road, Mount Clemens, Macomb County, MI

6. Photographic copy of floor plan drawing, dated August 18, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Photographic copy of floor plan drawing, dated August 18, 1968, Department of the Air Force Air Defense Command Installations, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. FLOOR PLAN BLDG. 1585. - Selfridge Field, Building No. 1585, South of East Joy Boulevard, west of Taxiway C, Mount Clemens, Macomb County, MI

5. Photographic copy of construction drawing, dated March (?), 1959, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Photographic copy of construction drawing, dated March (?), 1959, Department of the Air Force Air Defense Command Installations, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. FLOOR PLAN, SHEET 1 OF 1, DRAWING SLF-440-022. - Selfridge Field, Building No. 101, Supply Street east of Maple Street, Mount Clemens, Macomb County, MI

Photographic copy of floor plan drawing, dated March 7, 1968, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photographic copy of floor plan drawing, dated March 7, 1968, Department of the Air Force Air Defense Command Installations, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. FLOOR PLAN BLDG. 1575 - Selfridge Field, Building No. 1575, South of East Joy Boulevard, west of Taxiway C, Mount Clemens, Macomb County, MI

Computer Simulations of Epoxy Adhesive Monomer Interactions with Alumina Surfaces

DTIC Science & Technology

1992-08-01

using Sybyl"’ molecular modeling software 9 on a Digital Equipment Corporation microVAXw cluster. The Tripos force field was employed in the...Ave, N, Ft Leonard Wood, MO 65473-5000 1 ATM- Libary Commander, US. Army Enginer Waterways Expenment Station, P.O. Box 631, Vicksburg, MS 39180 1 ATTN

Comprehensive Base Realignment/Closure and Fort Belvoir Development

DTIC Science & Technology

1991-06-01

Passerina cyanea rufous-sided towhee Pipilo erythrophthalmus American tree sparrow Spizella arborea chipping sparrow Spizella passerina field... sparrow Spizella pusilla vesper sparrow Pooecetes gramineus savanah sparrow Passerculus sandwichensis fox sparrow Passerella iliaca song sparrow Melospiza...and Realignment Commission the relocation of selected information Systems command elements from Fort Belvoir to Fort Ritchie or another

Voyager backgrounder

NASA Technical Reports Server (NTRS)

1981-01-01

The Voyager spacecraft and experiments are described. The spacecraft description includes the structure and configuration, communications systems, power supplies, computer command subsystems, and the science platform. The experiments discussed are investigations of cosmic rays, low-energy charged particles, magnetic fields, and plasma waves, along with studies in radio astronomy photopolarimetry. The tracking and data acquisition procedures for the missions are presented.

Newsroom

Science.gov Websites

from Air Force Gen. Lori Robinson during a ceremony held at Peterson Air Force Base today. May 24, 2018 Gen. O'Shaughnessy assumes command of NORAD, USNORTHCOM Peterson Air Force Base, Colo. - Air Force Gen terminal Sept. 22, 2017 at Joint Base San Antonio-Kelly Field. The cargo was being staged at the Federal

The Gulf War: U.A.E. Participation in that War

DTIC Science & Technology

1993-04-02

and preserved out of the hollow force that had been allowed to develop in the 1970s. The U.S. Congress was also important in providing the democratic ... leadership support of the force in the field. The Middle East will continue to command attention for the future. The coalition partners in the Gulf

76 FR 3113 - Privacy Act of 1974; System of Records

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-19

... Force's notices for systems of records subject to the Privacy Act of 1974 (5 U.S.C. 552a), as amended... Center; major commands; field operating agencies; Military Personnel Sections at Air Force installations... mailing addresses are published as an appendix to the Air Force's compilation of systems of records...

Constraint-based semi-autonomy for unmanned ground vehicles using local sensing

NASA Astrophysics Data System (ADS)

Anderson, Sterling J.; Karumanchi, Sisir B.; Johnson, Bryan; Perlin, Victor; Rohde, Mitchell; Iagnemma, Karl

2012-06-01

Teleoperated vehicles are playing an increasingly important role in a variety of military functions. While advantageous in many respects over their manned counterparts, these vehicles also pose unique challenges when it comes to safely avoiding obstacles. Not only must operators cope with difficulties inherent to the manned driving task, but they must also perform many of the same functions with a restricted field of view, limited depth perception, potentially disorienting camera viewpoints, and significant time delays. In this work, a constraint-based method for enhancing operator performance by seamlessly coordinating human and controller commands is presented. This method uses onboard LIDAR sensing to identify environmental hazards, designs a collision-free path homotopy traversing that environment, and coordinates the control commands of a driver and an onboard controller to ensure that the vehicle trajectory remains within a safe homotopy. This system's performance is demonstrated via off-road teleoperation of a Kawasaki Mule in an open field among obstacles. In these tests, the system safely avoids collisions and maintains vehicle stability even in the presence of "routine" operator error, loss of operator attention, and complete loss of communications.

Coupled binding-bending-folding: The complex conformational dynamics of protein-DNA binding studied by atomistic molecular dynamics simulations.

PubMed

van der Vaart, Arjan

2015-05-01

Protein-DNA binding often involves dramatic conformational changes such as protein folding and DNA bending. While thermodynamic aspects of this behavior are understood, and its biological function is often known, the mechanism by which the conformational changes occur is generally unclear. By providing detailed structural and energetic data, molecular dynamics simulations have been helpful in elucidating and rationalizing protein-DNA binding. This review will summarize recent atomistic molecular dynamics simulations of the conformational dynamics of DNA and protein-DNA binding. A brief overview of recent developments in DNA force fields is given as well. Simulations have been crucial in rationalizing the intrinsic flexibility of DNA, and have been instrumental in identifying the sequence of binding events, the triggers for the conformational motion, and the mechanism of binding for a number of important DNA-binding proteins. Molecular dynamics simulations are an important tool for understanding the complex binding behavior of DNA-binding proteins. With recent advances in force fields and rapid increases in simulation time scales, simulations will become even more important for future studies. This article is part of a Special Issue entitled Recent developments of molecular dynamics. Copyright © 2014. Published by Elsevier B.V.

Self-assembly of multiferroic core-shell particulate nanocomposites through DNA-DNA hybridization and magnetic field directed assembly of superstructures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sreenivasulu, Gollapudi; Srinivasan, Gopalan, E-mail: srinivas@oakland.edu, E-mail: chavez@oakland.edu; Lochbiler, Thomas A.

Multiferroic composites of ferromagnetic and ferroelectric phases are of importance for studies on mechanical strain mediated coupling between the magnetic and electric subsystems. This work is on DNA-assisted self-assembly of superstructures of such composites with nanometer periodicity. The synthesis involved oligomeric DNA-functionalized ferroelectric and ferromagnetic nanoparticles, 600 nm BaTiO{sub 3} (BTO) and 200 nm NiFe{sub 2}O{sub 4} (NFO), respectively. Mixing BTO and NFO particles, possessing complementary DNA sequences, resulted in the formation of ordered core-shell heteronanocomposites held together by DNA hybridization. The composites were imaged by scanning electron microscopy and scanning microwave microscopy. The presence of heteroassemblies along with core-shellmore » architecture is clearly observed. The reversible nature of the DNA hybridization allows for restructuring the composites into mm-long linear chains and 2D-arrays in the presence of a static magnetic field and ring-like structures in a rotating-magnetic field. Strong magneto-electric (ME) coupling in as-assembled composites is evident from static magnetic field H induced polarization and low-frequency magnetoelectric voltage coefficient measurements. Upon annealing the nanocomposites at high temperatures, evidence for the formation of bulk composites with excellent cross-coupling between the electric and magnetic subsystems is obtained by H-induced polarization and low-frequency ME voltage coefficient. The ME coupling strength in the self-assembled composites is measured to be much stronger than in bulk composites with randomly distributed NFO and BTO prepared by direct mixing and sintering.« less

DNA-templated photonic arrays and assemblies: design principles and future opportunities.

PubMed

Su, Wu; Bonnard, Vanessa; Burley, Glenn A

2011-07-11

Molecular photonics is a rapidly developing and multi-disciplinary field of research involving the construction of molecular assemblies comprising photoactive building blocks that are responsive to a light stimulus. A salient challenge in this field is the controlled assembly of these building blocks with nanoscale precision. DNA exhibits considerable promise as an architecture for the templated assembly of photoactive materials. In this Concept Article we describe the progress that has been made in the area of DNA photonics, in which DNA acts as a platform for the construction of optoelectronic assemblies, thin films and devices. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

DNA repair: a changing geography? (1964-2008).

PubMed

Maisonobe, Marion; Giglia-Mari, Giuseppina; Eckert, Denis

2013-07-01

This article aims to explain the current state of DNA Repair studies' global geography by focusing on the genesis of the community. Bibliometric data is used to localize scientific activities related to DNA Repair at the city level. The keyword "DNA Repair" was introduced first by American scientists. It started to spread after 1964 that is to say, after P. Howard-Flanders (Yale University), P. Hanawalt (Stanford University) and R. Setlow (Oak Ridge Laboratories) found evidence for Excision Repair mechanisms. It was the first stage in the emergence of an autonomous scientific community. In this article, we will try to assess to what extent the geo-history of this scientific field is determinant in understanding its current geography. In order to do so, we will localize the places where the first "DNA Repair" publications were signed fifty years ago and the following spatial diffusion process, which led to the current geography of the field. Then, we will focus on the evolution of the research activity of "early entrants" in relation to the activity of "latecomers". This article is an opportunity to share with DNA Repair scientists some research results of a dynamic field in Science studies: spatial scientometrics. Copyright © 2013 Elsevier B.V. All rights reserved.

5. Command center doors at command center entry, building 501, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Command center doors at command center entry, building 501, looking north - Offutt Air Force Base, Strategic Air Command Headquarters & Command Center, Command Center, 901 SAC Boulevard, Bellevue, Sarpy County, NE

Fast DNA sieving through submicrometer cylindrical glass capillary matrix.

PubMed

Cao, Zhen; Yobas, Levent

2014-01-07

Here, we report on DNA electrophoresis through a novel artificial sieving matrix based on the highly regular submicrometer cylindrical glass capillary segments alternatingly arranged with wells. Such round capillaries pose a higher-order confinement resulting in a lower partition coefficient and greater entropic energy barrier while limiting the driving field strength to a small fraction of the applied electric field. In return, the separation can be performed at high average field strengths (up to 1.6 kV/cm) without encountering the field-dependent loss of resolving power. This leads to fast DNA sieving as demonstrated here on the capillaries of 750 nm in diameter. The 600 bp to 21 kbp long chains are shown to resolve within 4 min after having undergone a fairly limited number of entropic barriers (128 in total). The capillary matrix also exhibits a critical field threshold below which DNA bands fail to launch, and this occurs at a considerably greater magnitude than in other matrixes. The submicrometer capillaries are batch-fabricated on silicon through a fabrication process that does not require high-resolution advanced lithography or well-controlled wafer bonding techniques to define their critical dimension.

Network, system, and status software enhancements for the autonomously managed electrical power system breadboard. Volume 3: Commands specification

NASA Technical Reports Server (NTRS)

Mckee, James W.

1990-01-01

This volume (3 of 4) contains the specification for the command language for the AMPS system. The volume contains a requirements specification for the operating system and commands and a design specification for the operating system and command. The operating system and commands sits on top of the protocol. The commands are an extension of the present set of AMPS commands in that the commands are more compact, allow multiple sub-commands to be bundled into one command, and have provisions for identifying the sender and the intended receiver. The commands make no change to the actual software that implement the commands.

Pure field theories and MACSYMA algorithms

NASA Technical Reports Server (NTRS)

Ament, W. S.

1977-01-01

A pure field theory attempts to describe physical phenomena through singularity-free solutions of field equations resulting from an action principle. The physics goes into forming the action principle and interpreting specific results. Algorithms for the intervening mathematical steps are sketched. Vacuum general relativity is a pure field theory, serving as model and providing checks for generalizations. The fields of general relativity are the 10 components of a symmetric Riemannian metric tensor; those of the Einstein-Straus generalization are the 16 components of a nonsymmetric. Algebraic properties are exploited in top level MACSYMA commands toward performing some of the algorithms of that generalization. The light cone for the theory as left by Einstein and Straus is found and simplifications of that theory are discussed.

Joint Chiefs of Staff > Media > Photos

Science.gov Websites

U.S. Army Gen. Curtis M. Scaparrotti, left, Commander of U.S. European Command and Supreme Allied , Commander of U.S. European Command and Supreme Allied Commander, Europe; speaks after receiving the . U.S. Army Gen. Curtis M. Scaparrotti, Commander of U.S. European Command and Supreme Allied Commander

Ancient DNA studies: new perspectives on old samples

PubMed Central

2012-01-01

In spite of past controversies, the field of ancient DNA is now a reliable research area due to recent methodological improvements. A series of recent large-scale studies have revealed the true potential of ancient DNA samples to study the processes of evolution and to test models and assumptions commonly used to reconstruct patterns of evolution and to analyze population genetics and palaeoecological changes. Recent advances in DNA technologies, such as next-generation sequencing make it possible to recover DNA information from archaeological and paleontological remains allowing us to go back in time and study the genetic relationships between extinct organisms and their contemporary relatives. With the next-generation sequencing methodologies, DNA sequences can be retrieved even from samples (for example human remains) for which the technical pitfalls of classical methodologies required stringent criteria to guaranty the reliability of the results. In this paper, we review the methodologies applied to ancient DNA analysis and the perspectives that next-generation sequencing applications provide in this field. PMID:22697611

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review).

PubMed

Tao, Ruiyang; Wang, Shouyu; Zhang, Jiashuo; Zhang, Jingyi; Yang, Zihao; Sheng, Xiang; Hou, Yiping; Zhang, Suhua; Li, Chengtao

2018-05-25

Interpreting mixed DNA samples containing material from multiple contributors has long been considered a major challenge in forensic casework, especially when encountering low-template DNA (LT-DNA) or high-order mixtures that may involve missing alleles (dropout) and unrelated alleles (drop-in), among others. In the last decades, extraordinary progress has been made in the analysis of mixed DNA samples, which has led to increasing attention to this research field. The advent of new methods for the separation and extraction of DNA from mixtures, novel or jointly applied genetic markers for detection and reliable interpretation approaches for estimating the weight of evidence, as well as the powerful massively parallel sequencing (MPS) technology, has greatly extended the range of mixed samples that can be correctly analyzed. Here, we summarized the investigative approaches and progress in the field of forensic DNA mixture analysis, hoping to provide some assistance to forensic practitioners and to promote further development involving this issue.

A reply to Iversen et al.'s comment “Monitoring of animal abundance by environmental DNA - An increasingly obscure perspective”

USGS Publications Warehouse

Klymus, Katy E.; Richter, Catherine A.; Chapman, Duane C.; Paukert, Craig P.

2015-01-01

We appreciate the conversation put forward by Iversen et al. (2015) in their response to our article “Quantification of eDNA shedding rates from invasive bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix” in the 2015 environmental DNA special issue of Biological Conservation.We agree with Iversen et al.'s concern about overly optimistic conclusions that could be drawn from the current eDNA literature. One hope for eDNA technology is that it can be used in estimating abundance or population density. Evidence suggests that eDNA measurements correlate with total biomass (Takahara et al., 2012) rather than abundance. We demonstrate a similar relationship between biomass and eDNA shedding rates. Nevertheless, without field testing of these methods and specific survey protocols, we cannot make strong conclusions regarding the technique's field applicability. In our manuscript, we attempted to point out areas in which more research is needed.

Critical considerations for the application of environmental DNA methods to detect aquatic species

USGS Publications Warehouse

Goldberg, Caren S.; Turner, Cameron R.; Deiner, Kristy; Klymus, Katy E.; Thomsen, Philip Francis; Murphy, Melanie A.; Spear, Stephen F.; McKee, Anna; Oyler-McCance, Sara J.; Cornman, Robert S.; Laramie, Matthew B.; Mahon, Andrew R.; Lance, Richard F.; Pilliod, David S.; Strickler, Katherine M.; Waits, Lisette P.; Fremier, Alexander K.; Takahara, Teruhiko; Herder, Jelger E.; Taberlet, Pierre

2016-01-01

Species detection using environmental DNA (eDNA) has tremendous potential for contributing to the understanding of the ecology and conservation of aquatic species. Detecting species using eDNA methods, rather than directly sampling the organisms, can reduce impacts on sensitive species and increase the power of field surveys for rare and elusive species. The sensitivity of eDNA methods, however, requires a heightened awareness and attention to quality assurance and quality control protocols. Additionally, the interpretation of eDNA data demands careful consideration of multiple factors. As eDNA methods have grown in application, diverse approaches have been implemented to address these issues. With interest in eDNA continuing to expand, supportive guidelines for undertaking eDNA studies are greatly needed.Environmental DNA researchers from around the world have collaborated to produce this set of guidelines and considerations for implementing eDNA methods to detect aquatic macroorganisms.Critical considerations for study design include preventing contamination in the field and the laboratory, choosing appropriate sample analysis methods, validating assays, testing for sample inhibition and following minimum reporting guidelines. Critical considerations for inference include temporal and spatial processes, limits of correlation of eDNA with abundance, uncertainty of positive and negative results, and potential sources of allochthonous DNA.We present a synthesis of knowledge at this stage for application of this new and powerful detection method.

HmtDB 2016: data update, a better performing query system and human mitochondrial DNA haplogroup predictor.

PubMed

Clima, Rosanna; Preste, Roberto; Calabrese, Claudia; Diroma, Maria Angela; Santorsola, Mariangela; Scioscia, Gaetano; Simone, Domenico; Shen, Lishuang; Gasparre, Giuseppe; Attimonelli, Marcella

2017-01-04

The HmtDB resource hosts a database of human mitochondrial genome sequences from individuals with healthy and disease phenotypes. The database is intended to support both population geneticists as well as clinicians undertaking the task to assess the pathogenicity of specific mtDNA mutations. The wide application of next-generation sequencing (NGS) has provided an enormous volume of high-resolution data at a low price, increasing the availability of human mitochondrial sequencing data, which called for a cogent and significant expansion of HmtDB data content that has more than tripled in the current release. We here describe additional novel features, including: (i) a complete, user-friendly restyling of the web interface, (ii) links to the command-line stand-alone and web versions of the MToolBox package, an up-to-date tool to reconstruct and analyze human mitochondrial DNA from NGS data and (iii) the implementation of the Reconstructed Sapiens Reference Sequence (RSRS) as mitochondrial reference sequence. The overall update renders HmtDB an even more handy and useful resource as it enables a more rapid data access, processing and analysis. HmtDB is accessible at http://www.hmtdb.uniba.it/. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Design and Development of a Technology Platform for DNA-Encoded Library Production and Affinity Selection.

PubMed

Castañón, Jesús; Román, José Pablo; Jessop, Theodore C; de Blas, Jesús; Haro, Rubén

2018-06-01

DNA-encoded libraries (DELs) have emerged as an efficient and cost-effective drug discovery tool for the exploration and screening of very large chemical space using small-molecule collections of unprecedented size. Herein, we report an integrated automation and informatics system designed to enhance the quality, efficiency, and throughput of the production and affinity selection of these libraries. The platform is governed by software developed according to a database-centric architecture to ensure data consistency, integrity, and availability. Through its versatile protocol management functionalities, this application captures the wide diversity of experimental processes involved with DEL technology, keeps track of working protocols in the database, and uses them to command robotic liquid handlers for the synthesis of libraries. This approach provides full traceability of building-blocks and DNA tags in each split-and-pool cycle. Affinity selection experiments and high-throughput sequencing reads are also captured in the database, and the results are automatically deconvoluted and visualized in customizable representations. Researchers can compare results of different experiments and use machine learning methods to discover patterns in data. As of this writing, the platform has been validated through the generation and affinity selection of various libraries, and it has become the cornerstone of the DEL production effort at Lilly.

The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol-chloroform-isoamyl alcohol DNA extraction.

PubMed

Renshaw, Mark A; Olds, Brett P; Jerde, Christopher L; McVeigh, Margaret M; Lodge, David M

2015-01-01

Current research targeting filtered macrobial environmental DNA (eDNA) often relies upon cold ambient temperatures at various stages, including the transport of water samples from the field to the laboratory and the storage of water and/or filtered samples in the laboratory. This poses practical limitations for field collections in locations where refrigeration and frozen storage is difficult or where samples must be transported long distances for further processing and screening. This study demonstrates the successful preservation of eDNA at room temperature (20 °C) in two lysis buffers, CTAB and Longmire's, over a 2-week period of time. Moreover, the preserved eDNA samples were seamlessly integrated into a phenol-chloroform-isoamyl alcohol (PCI) DNA extraction protocol. The successful application of the eDNA extraction to multiple filter membrane types suggests the methods evaluated here may be broadly applied in future eDNA research. Our results also suggest that for many kinds of studies recently reported on macrobial eDNA, detection probabilities could have been increased, and at a lower cost, by utilizing the Longmire's preservation buffer with a PCI DNA extraction. © 2014 The Authors. Molecular Ecology Resources Published by John Wiley & Sons Ltd.

Fast kinetic studies of plasmid DNA transfer in intact yeast cells mediated by electropulsation.

PubMed

Ganeva, V; Galutzov, B; Teissie, J

1995-09-25

Intact yeast cell Electrotransformation process was investigated. It is a two step process. The plasmid must be pre-mixed and present in contact with the cells during the pulse. During the millisecond field pulse, plasmid DNA is associated to the envelope. It therefore crosses the membrane by a process which lasts several seconds as shown by its sensitivity to a post pulse addition of DNase. Electrotransformation is not supported by an electrophoretic transfer due to the external field nor by a free diffusion across the electropermeabilized envelope. DNA is first bound during the field pulse and then is transferred by a still unknown active process due to cell metabolism.

Direct electrochemical stripping detection of cystic-fibrosis-related DNA linked through cadmium sulfide quantum dots

NASA Astrophysics Data System (ADS)

Marin, Sergio; Merkoçi, Arben

2009-02-01

Electrochemical detection of a cadmium sulfide quantum dots (CdS QDs)-DNA complex connected to paramagnetic microbeads (MB) was performed without the need for chemical dissolving. The method is based on dropping 20 µl of CdS QD-DNA-MB suspension on the surface of a screen-printed electrode. It is followed by magnetic collection on the surface of the working electrode and electrochemical detection using square-wave voltammetry (SWV), giving a well-shaped and sensitive analytical signal. A cystic-fibrosis-related DNA sequence was sandwiched between the two DNA probes. One DNA probe is linked via biotin-streptavidin bonding with MB and the other one via thiol groups with the CdS QD used as tags. Nonspecific signals of DNA were minimized using a blocking agent and the results obtained were successfully employed in a model DNA sensor with an interest in future applications in the clinical field. The developed nanoparticle biosensing system may offer numerous opportunities in other fields where fast, low cost and efficient detection of small volume samples is required.

32 CFR 700.1053 - Commander of a task force.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false Commander of a task force. 700.1053 Section 700... Command Detail to Duty § 700.1053 Commander of a task force. (a) A geographic fleet commander, and any other naval commander, may detail in command of a task force, or other task command, any eligible...

Silicene nanoribbon as a new DNA sequencing device

NASA Astrophysics Data System (ADS)

Alesheikh, Sara; Shahtahmassebi, Nasser; Roknabadi, Mahmood Rezaee; Pilevar Shahri, Raheleh

2018-02-01

The importance of applying DNA sequencing in different fields, results in looking for fast and cheap methods. Nanotechnology helps this development by introducing nanostructures used for DNA sequencing. In this work we study the interaction between zigzag silicene nanoribbon and DNA nucleobases using DFT and non equilibrium Green's function approach, to investigate the possibility of using zigzag silicene nanoribbons as a biosensor for DNA sequencing.

Desert Rats 2010 Operations Tests: Insights from the Geology Crew Members

NASA Technical Reports Server (NTRS)

Bleacher, J. E.; Hurtado, J. M., Jr.; Young, K. E.; Rice, J.; Garry, W. B.; Eppler, D.

2011-01-01

Desert Research and Technology Studies (Desert RATS) is a multi-year series of tests of NASA hardware and operations deployed in the high desert of Arizona. Conducted annually since 1997, these activities exercise planetary surface hardware and operations in relatively harsh conditions where long-distance, multi-day roving is achievable. Such activities not only test vehicle subsystems, they also stress communications and operations systems and enable testing of science operations approaches that advance human and robotic surface exploration capabilities. Desert RATS 2010 tested two crewed rovers designed as first-generation prototypes of small pressurized vehicles, consistent with exploration architecture designs. Each rover provided the internal volume necessary for crewmembers to live and work for periods up to 14 days, as well as allowing for extravehicular activities (EVAs) through the use of rear-mounted suit ports. The 2010 test was designed to simulate geologic science traverses over a 14-day period through a volcanic field that is analogous to volcanic terrains observed throughout the Solar System. The test was conducted between 31 August and 13 September 2010. Two crewmembers lived in and operated each rover for a week with a "shift change" on day 7, resulting in a total of eight test subjects for the two-week period. Each crew consisted of an engineer/commander and an experienced field geologist. Three of the engineer/commanders were experienced astronauts with at least one Space Shuttle flight. The field geologists were drawn from the scientific community, based on funded and published field expertise.

Investigation on the diagnostic sensitivity of molecular tools used for detection of koi herpesvirus.

PubMed

Bergmann, Sven M; Riechardt, Meike; Fichtner, Dieter; Lee, Peiyu; Kempter, Jolanta

2010-02-01

Previous and new PCRs for KHV detection were compared by estimation of their sensitivity in recognizing KHV DNA in plasmids, cell culture extracted KHV DNA and total DNA obtained from field tissue samples. A modified real-time PCR (Gilad et al., 2004), combined with an internal control system (IC2, Hoffmann et al., 2006) in a duplex assay, was used as a "gold standard". The lowest reliably determined virus concentration between, 5 and 10 KHV DNA genomic equivalents, was found by real-time PCR (Gilad et al., 2004), nested PCR (Bergmann et al., 2006) and one-tube semi-nested PCR. All other published and unpublished PCRs, as well as the commercial Loopamp, recognized KHV DNA at higher concentrations only. Additionally, KHV variants, newly adapted to European conditions, which could not be detected by PCR according to Bercovier et al. (2005) were found in two field samples from carp and koi from different regions of Germany. A negative influence of sample pooling was shown with field samples tested by real-time PCR. 2009 Elsevier B.V. All rights reserved.

STS-85 Cmdr Brown addresses media during TCDT

NASA Technical Reports Server (NTRS)

1997-01-01

STS-85 Commander Curtis L. Brown, Jr., addresses the news media at a briefing at Launch Pad 39A while the other members of the flight crew in the background prepare to field questions during a break in Terminal Countdown Demonstration Test (TCDT) activities for that mission. They are (back row, from left): Pilot Kent V. Rominger; Payload Commander N. Jan Davis; Mission Specialist Stephen K. Robinson; Payload Specialist Bjarni V. Tryggvason; and Mission Specialist Robert L. Curbeam, Jr. The primary payload aboard the Space Shuttle orbiter Discovery is the Cryogenic Infrared Spectrometers and Telescopes for the Atmosphere-2 (CRISTA-SPAS-2). Other payloads on the 11-day mission include the Manipulator Flight Demonstration (MFD), and Technology Applications and Science-1 (TAS-1) and International Extreme Ultraviolet Hitchhiker-2 (IEH-2) experiments.

STS-92 Commander Duffy suits up

NASA Technical Reports Server (NTRS)

2000-01-01

STS-92 Commander Brian Duffy has his launch and entry suit checked before launch, scheduled for 8:05 p.m. EDT. The mission is the fifth flight for the construction of the ISS. The payload includes the Integrated Truss Structure Z-1 and the third Pressurized Mating Adapter. During the 11-day mission, four extravehicular activities (EVAs), or spacewalks, are planned. The Z-1 truss is the first of 10 that will become the backbone of the International Space Station, eventually stretching the length of a football field. PMA-3 will provide a Shuttle docking port for solar array installation on the sixth ISS flight and Lab installation on the seventh ISS flight. This launch is the fourth for Duffy. Landing is expected Oct. 21 at 3:55 p.m. EDT.

Protocol for quantitative tracing of surface water with synthetic DNA

NASA Astrophysics Data System (ADS)

Foppen, J. W.; Bogaard, T. A.

2012-04-01

Based on experiments we carried out in 2010 with various synthetic single stranded DNA markers with a size of 80 nucleotides (ssDNA; Foppen et al., 2011), we concluded that ssDNA can be used to carry out spatially distributed multi-tracer experiments in the environment. Main advantages are in principle unlimited amount of tracers, environmental friendly and tracer recovery at very high dilution rates (detection limit is very low). However, when ssDNA was injected in headwater streams, we found that at selected downstream locations, the total mass recovery was less than 100%. The exact reason for low mass recovery was unknown. In order to start identifying the cause of the loss of mass in these surface waters, and to increase our knowledge of the behaviour of synthetic ssDNA in the environment, we examined the effect of laboratory and field protocols working with artificial DNA by performing numerous batch experiments. Then, we carried out several field tests in different headwater streams in the Netherlands and in Luxembourg. The laboratory experiments consisted of a batch of water in a vessel with in the order of 10^10 ssDNA molecules injected into the batch. The total duration of each experiment was 10 hour, and, at regular time intervals, 100 µl samples were collected in a 1.5 ml Eppendorf vial for qPCR analyses. The waters we used ranged from milliQ water to river water with an Electrical Conductivity of around 400 μS/cm. The batch experiments were performed in different vessel types: polyethylene bottles, polypropylene copolymer bottles , and glass bottles. In addition, two filter types were tested: 1 µm pore size glass fibre filters and 0.2 µm pore size cellulose acetate filters. Lastly, stream bed sediment was added to the batch experiments to quantify interaction of the DNA with sediment. For each field experiment around 10^15 ssDNA molecules were injected, and water samples were collected 100 - 600 m downstream of the point of injection. Additionally, the field tests were performed with salt and deuterium as tracer. To study possible decay by sunlight and/or microbial activity for synthetic DNA, immediately in the field and for the duration of the entire experiment, we carried out batch experiments. All samples were stored in a 1.5 ml Eppendorf vial in a cool-box in dry ice (-80°C). Quantitative PCR on a Mini Opticon (Bio Rad, Hercules, CA, USA) was carried out to determine DNA concentrations in the samples. Results showed the importance of a strict protocol for working with ssDNA as a tracer for quantitative tracing, since ssDNA interacts with surface areas of glass and plastic, depending on water quality and ionic strength. Interaction with the sediment and decay due to sunlight and/or microbial activity was negligible in most cases. The ssDNA protocol was then tested in natural streams. Promising results were obtained using ssDNA as quantitative tracer. The breakthrough curves using ssDNA were similar to the ones of salt or deuterium. We will present the revised protocol to use ssDNA for multi-tracing experiments in natural streams and discuss the opportunities and limitations.

Extremely low-frequency electromagnetic fields cause DNA strand breaks in normal cells

PubMed Central

2014-01-01

Background Extremely low frequency electromagnetic fields aren’t considered as a real carcinogenic agent despite the fact that some studies have showed impairment of the DNA integrity in different cells lines. The aim of this study was evaluation of the late effects of a 100 Hz and 5.6 mT electromagnetic field, applied continuously or discontinuously, on the DNA integrity of Vero cells assessed by alkaline Comet assay and by cell cycle analysis. Normal Vero cells were exposed to extremely low frequency electromagnetic fields (100 Hz, 5.6 mT) for 45 minutes. The Comet assay and cell cycle analysis were performed 48 hours after the treatment. Results Exposed samples presented an increase of the number of cells with high damaged DNA as compared with non-exposed cells. Quantitative evaluation of the comet assay showed a significantly (<0.001) increase of the tail lengths, of the quantity of DNA in tail and of Olive tail moments, respectively. Cell cycle analysis showed an increase of the frequency of the cells in S phase, proving the occurrence of single strand breaks. The most probable mechanism of induction of the registered effects is the production of different types of reactive oxygen species. Conclusions The analysis of the registered comet indices and of cell cycle showed that extremely low frequency electromagnetic field of 100 Hz and 5.6 mT had a genotoxic impact on Vero cells. PMID:24401758

The C3-System User. Volume II. Workshop Notes

DTIC Science & Technology

1977-02-01

system that provides the means for operational direction and technical administrative support involved in the function of command and control of U.S...information systems of the Headquarters of the Military Depart- ments; the command and control systems of the Headquarters of the Service Component Commands...the Service Component Commands - Military Airlift Command - Military Sealift Command - Military Traffic Management Command - 3.2.5 Command and

32 CFR 700.703 - To announce assumption of command.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false To announce assumption of command. 700.703... Chief and Other Commanders Titles and Duties of Commanders § 700.703 To announce assumption of command. (a) Upon assuming command, commanders shall so advise appropriate superiors, and the units of their...

32 CFR 700.703 - To announce assumption of command.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false To announce assumption of command. 700.703... Chief and Other Commanders Titles and Duties of Commanders § 700.703 To announce assumption of command. (a) Upon assuming command, commanders shall so advise appropriate superiors, and the units of their...

32 CFR 536.8 - Responsibilities and operations of command claims services.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Responsibilities and operations of command claims... operations of command claims services. (a) Chiefs of command claims services. Chiefs of command claims... Commander USARCS, and assigned an office code. However, the chief of a command claims service may...

32 CFR 700.703 - To announce assumption of command.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 5 2014-07-01 2014-07-01 false To announce assumption of command. 700.703... Chief and Other Commanders Titles and Duties of Commanders § 700.703 To announce assumption of command. (a) Upon assuming command, commanders shall so advise appropriate superiors, and the units of their...

32 CFR 700.703 - To announce assumption of command.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false To announce assumption of command. 700.703... Chief and Other Commanders Titles and Duties of Commanders § 700.703 To announce assumption of command. (a) Upon assuming command, commanders shall so advise appropriate superiors, and the units of their...

32 CFR 700.703 - To announce assumption of command.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false To announce assumption of command. 700.703... Chief and Other Commanders Titles and Duties of Commanders § 700.703 To announce assumption of command. (a) Upon assuming command, commanders shall so advise appropriate superiors, and the units of their...

32 CFR 536.8 - Responsibilities and operations of command claims services.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true Responsibilities and operations of command claims... operations of command claims services. (a) Chiefs of command claims services. Chiefs of command claims... Commander USARCS, and assigned an office code. However, the chief of a command claims service may...

32 CFR 536.8 - Responsibilities and operations of command claims services.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 3 2012-07-01 2009-07-01 true Responsibilities and operations of command claims... operations of command claims services. (a) Chiefs of command claims services. Chiefs of command claims... Commander USARCS, and assigned an office code. However, the chief of a command claims service may...

8. Photographic copy of construction drawing, dated January 10, 1962, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Photographic copy of construction drawing, dated January 10, 1962, Department of the Air Force Air Defense Command Installations for Selfridge, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. ELEVATIONS AND SECTIONS, SLF 140-056 SHEET 3 OF 7. - Selfridge Field, Building No. 562, Ammo Road northeast of Taxiway A, Mount Clemens, Macomb County, MI

Photographic copy of construction drawing, dated November 17, 1961, Department ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photographic copy of construction drawing, dated November 17, 1961, Department of the Air Force, Air Defense Command Installations, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. FLOOR PLAN, EQUIPMENT LAYOUT, SITE PLAN, DRAWING NO. P20-130-002 - Selfridge Field, Building No. 1025, Doolittle Drive north of D Street, Mount Clemens, Macomb County, MI

7. Photographic copy of construction drawing, dated January 10, 1962, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photographic copy of construction drawing, dated January 10, 1962, Department of the Air Force Air Defense Command Installations for Selfridge, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. ARCHITECTURAL PLAN AND SCHEDULES, SLF 140-056, SHEET 2 OF 7. - Selfridge Field, Building No. 562, Ammo Road northeast of Taxiway A, Mount Clemens, Macomb County, MI

Photographic copy of plan drawing, Department of the Air Force, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photographic copy of plan drawing, Department of the Air Force, Air Defense Command Directorate of Installations, May 28, 1954, Civil Engineers Office, in possession of Selfridge Base Museum, Mt. Clemens, Michigan. FLOOR PLAN, 826-198-4 - Selfridge Field, Building No. 199, South of George Avenue between Walnut & Birch Streets, Mount Clemens, Macomb County, MI

Defining Stability, Security, Transition, and Reconstruction (SSTR) Operations Requirements for Future Department of the Navy Training and Analytical Models and Simulations

DTIC Science & Technology

2008-09-01

43 B. WELL-DEFINED MEASURES ........................................................... 43 C. ESSENTIAL ELEMENTS OF ANALYSIS ( EEA ...45 D. EEA PROCESS FOR RESTORATION OF ESSENTIAL SERVICE - WATER...FBCB2 Force XXI Battle Command, Brigade-and-Below FM Army Field Manual EEA Essential Elements of Analysis EPG Electronic Proving Ground ESS

Report on Research at AFGL July 1976 - December 1978

DTIC Science & Technology

1980-11-01

Research at the Air Force Geophysics Laboratory . This report covers a two-and-one-half... Laboratories (AFCRL). The Air Force redesignated AFCRL to AFGL on January 15, 1976, in order to focus attention and effort into geophysics research and...USAF Commander Contents Th» Air Foro* GMphyalc« Laboratory C rganization and People . . . Annual Budgets . . . Field Sites . . . Research

Alternative Energy and Propulsion Power for Today’s US Military

DTIC Science & Technology

2009-05-05

the Problem ........................................................................................4 Real Illustrations of the Problem ...taken to reduce its grip on fossil fuels. Further Defining the Problem In 2006 testimony before the US Congress, a DoD representative stated that...necessities of our military.12 Real Illustrations of the Problem Warfighting commanders in the field are requesting alternatives to petroleum based energy. In

Lexical Errors in Second Language Scientific Writing: Some Conceptual Implications

ERIC Educational Resources Information Center

Carrió Pastor, María Luisa; Mestre-Mestre, Eva María

2014-01-01

Nowadays, scientific writers are required not only a thorough knowledge of their subject field, but also a sound command of English as a lingua franca. In this paper, the lexical errors produced in scientific texts written in English by non-native researchers are identified to propose a classification of the categories they contain. This study…

32 CFR 22.710 - Assignment of grants administration offices.

Code of Federal Regulations, 2010 CFR

2010-07-01

... accessed through the Defense Contract Management Agency hompage at http://www.dcma.mil. (a) Regional... organizations are subject to the university cost principles in OMB Circular A-21. 11 11 See footnote 5 to § 22... footnote 5 to § 22.420(b)(1). (b) Field offices of the Defense Contract Management Command, for grants and...

On the Value of a Professional Seminar for Advertising Majors.

ERIC Educational Resources Information Center

Taylor, Ronald E.; Jackson, DeForrest

Advertising programs have an obligation to teach job search skills to their students and it is in their own best interests to do so. The rationale for this is threefold. First, advertising programs must command the respect of the professionals in the field, because professionals represent the employment market and are increasingly becoming sources…

An Analysis of the Impact of Financial Factors on the Well-Being of Military Officers

DTIC Science & Technology

2015-12-01

34 Figure 18. Job Fields of Participants ...........................................................................34 Figure 19...financial environment as their civilian counterparts, but with some unique challenges and unique benefits. Benefits include relative job stability...instruction goes on to state that personal financial management services shall support the commander, promote readiness and retention , include education

The SIQ-III Test: Gender Issues in Literacy

ERIC Educational Resources Information Center

Cassidy, Jack; Garcia, Roberto; Boggs, Merry

2005-01-01

The authors address concern in the field today about the literacy needs of boys. In a 1977 precursor to this article, it was literacy issues related to girls that appeared to command attention. As in that article and another preceding one, information is presented here as a true-false test. After taking the test, readers are provided with answers…

Transformation of Marine Corps Artillery in Support of the 2015 Expeditionary Force

DTIC Science & Technology

2008-01-01

artillery electronics maintenance, and meterological sections in support ofsubordinate elements. On order, the Regiment assumes the primary civil...order capability), engineer, counterbattery radar, artillery electronics maintenance, and meterological sections in support ofsubordinate elements...www.tecom.usmc.mil. (accessed December 15,2007). Training and Education Command. MOS Roadmap: 0847 - Field Artillery Meterological Crew Member. Quantico

Efficiency vs. Effectiveness: Can W. Edwards Deming's Principles of Quality Management Be Applied Successfully to American Education.

ERIC Educational Resources Information Center

Petry, John R.

The field of education has been slow to recognize the Total Quality Management (TQM) concept. This resistance may result from entrenched management styles characterized by hierarchical decision-making structures. TQM emphasizes management based on leadership instead of management by objective, command, and coercion. The TQM concept consists of…

Expeditionary Economics: The Military’s Role in Conflict-Related Development

DTIC Science & Technology

2012-06-08

self-sustaining economy , transition to responsible and productive member of the international community. It is important to note that there is...Emergency Response Program COIN Counterinsurgency COMISAF Commander International Security Assistance Force CORDS Civil Operations and...Foreign Internal Defense FM Field Manual GDP Gross Domestic Product IGO Intergovernmental Organization JIIM Joint, Interagency, Intergovernmental

Media Influence and its Effects on Military Operations

DTIC Science & Technology

1998-01-01

when journalists spend significant amounts of time with Marine units at the company and platoon level. This results in more accurate reporting ...with the reporting process. 7. Under conditions of open coverage, field commanders will permit journalists to ride on military vehicles and...41 Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting burden for

USV Path Planning Using Potential Field Model

DTIC Science & Technology

2017-09-01

control system and hardware used. Figure 24. Control System Block Diagram The course commands are passed via Wi - Fi to the USV where the low...plane about the center. The estimated moment of inertia is 2 2 2 2 2( ) 36(1.35 0.98 ) 8.35 12 12 m b wI kg m+ += = = • . (41) b. Added

Training Reflective Processes in Military Aircrews through Holistic Debriefing: The Importance of Facilitator Skills and Development of Trust

ERIC Educational Resources Information Center

Moldjord, Christian; Hybertsen, Ingunn Dahler

2015-01-01

This paper explores how Holistic Debrief, a new concept in the field of debriefing and reflective processes, can contribute to restitution, reflection and learning in professional teams following stressful events and routine tasks. Interviews were conducted with Norwegian military aircrew mission commanders following deployment to Afghanistan in…

STS-135_VMS

NASA Image and Video Library

2011-03-02

JSC2011-E-040202 (2 March 2011) --- NASA astronaut Chris Ferguson, STS-135 commander, departs from the Moffett Field (Calif.) flight operations center for his trip home to Houston after he and his crew trained in the Vertical Motion Simulator (VMS) at NASA's Ames Research Center in Mountain View, March 2, 2011. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

STS-135_VMS

NASA Image and Video Library

2011-03-02

JSC2011-E-040204 (2 March 2011) --- NASA astronaut Chris Ferguson, STS-135 commander, prepares for departure from Moffett Field in a T-38 trainer home to Houston after the crew of STS-135 trained in the Vertical Motion Simulator (VMS) at NASA's Ames Research Center in Mountain View, Calif. on March 2, 2011, Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

A Unit History of the 315th Bomb Wing: 1944-1946

DTIC Science & Technology

1986-03-01

Commanding Officer down to the newest buck private, of a coming rendezvous with destiny , one would have to come to its present location at Robins Field...tired, hungry, and grimy men reached the pitch black confines of the embryo airfield, loaded with one another’s gear and equipment." (38:Apr 45) Col

KSC-03pd1117

NASA Image and Video Library

2003-04-09

KENNEDY SPACE CENTER, FLA. -- John Lopez of Bristol, Texas, a crew boss for a camp crew at the Nacogdoches site, looks over tents used by U.S. Forest Service workers in the field searching for parts of Columbia. Kennedy Space Center workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

The United States Navy Arctic Roadmap for 2014 to 2030

DTIC Science & Technology

2014-02-01

of the Oceanographer of the Navy; the Chief of Naval Research; Commander, Naval Meteorology and Oceanography Command; Commander, Office of Naval...Q3, FY14 Q3, FY15 FY15-18 FY18 2.3.4: Improve traditional meteorological forecast capability in the polar regions through the...CNE Commander Naval Forces Europe CNIC Commander Navy Installations Command CNMOC Commander Naval Meteorology and Oceanography Command CNO Chief

32 CFR 536.12 - Commanding General, U.S. Army Medical Command.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true Commanding General, U.S. Army Medical Command... AND ACCOUNTS CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.12 Commanding General, U.S. Army Medical Command. (a) After consulting with the Commander USARCS on the selection of medical claims...

32 CFR 536.14 - Commanders of major Army commands.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 3 2012-07-01 2009-07-01 true Commanders of major Army commands. 536.14 Section... CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.14 Commanders of major Army commands... respective commands for compliance with the responsibilities assigned in §§ 536.9 and 536.10. (b) Assist...

32 CFR 536.12 - Commanding General, U.S. Army Medical Command.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 3 2012-07-01 2009-07-01 true Commanding General, U.S. Army Medical Command... AND ACCOUNTS CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.12 Commanding General, U.S. Army Medical Command. (a) After consulting with the Commander USARCS on the selection of medical claims...

32 CFR 536.14 - Commanders of major Army commands.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Commanders of major Army commands. 536.14 Section... CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.14 Commanders of major Army commands... respective commands for compliance with the responsibilities assigned in §§ 536.9 and 536.10. (b) Assist...

32 CFR 536.14 - Commanders of major Army commands.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 3 2013-07-01 2013-07-01 false Commanders of major Army commands. 536.14... ACCOUNTS CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.14 Commanders of major Army commands... respective commands for compliance with the responsibilities assigned in §§ 536.9 and 536.10. (b) Assist...

32 CFR 536.14 - Commanders of major Army commands.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true Commanders of major Army commands. 536.14 Section... CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.14 Commanders of major Army commands... respective commands for compliance with the responsibilities assigned in §§ 536.9 and 536.10. (b) Assist...

32 CFR 536.12 - Commanding General, U.S. Army Medical Command.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 3 2014-07-01 2014-07-01 false Commanding General, U.S. Army Medical Command... AND ACCOUNTS CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.12 Commanding General, U.S. Army Medical Command. (a) After consulting with the Commander USARCS on the selection of medical claims...

32 CFR 536.12 - Commanding General, U.S. Army Medical Command.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Commanding General, U.S. Army Medical Command... AND ACCOUNTS CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.12 Commanding General, U.S. Army Medical Command. (a) After consulting with the Commander USARCS on the selection of medical claims...

32 CFR 536.14 - Commanders of major Army commands.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 3 2014-07-01 2014-07-01 false Commanders of major Army commands. 536.14... ACCOUNTS CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.14 Commanders of major Army commands... respective commands for compliance with the responsibilities assigned in §§ 536.9 and 536.10. (b) Assist...

32 CFR 536.12 - Commanding General, U.S. Army Medical Command.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 3 2013-07-01 2013-07-01 false Commanding General, U.S. Army Medical Command... AND ACCOUNTS CLAIMS AGAINST THE UNITED STATES The Army Claims System § 536.12 Commanding General, U.S. Army Medical Command. (a) After consulting with the Commander USARCS on the selection of medical claims...

Autonomous Onboard Science Image Analysis for Future Mars Rover Missions

NASA Technical Reports Server (NTRS)

Gulick, V. C.; Morris, R. L.; Ruzon, M. A.; Roush, T. L.

1999-01-01

To explore high priority landing sites and to prepare for eventual human exploration, future Mars missions will involve rovers capable of traversing tens of kilometers. However, the current process by which scientists interact with a rover does not scale to such distances. Specifically, numerous command cycles are required to complete even simple tasks, such as, pointing the spectrometer at a variety of nearby rocks. In addition, the time required by scientists to interpret image data before new commands can be given and the limited amount of data that can be downlinked during a given command cycle constrain rover mobility and achievement of science goals. Experience with rover tests on Earth supports these concerns. As a result, traverses to science sites as identified in orbital images would require numerous science command cycles over a period of many weeks, months or even years, perhaps exceeding rover design life and other constraints. Autonomous onboard science analysis can address these problems in two ways. First, it will allow the rover to transmit only "interesting" images, defined as those likely to have higher science content. Second, the rover will be able to anticipate future commands. For example, a rover might autonomously acquire and return spectra of "interesting" rocks along with a high resolution image of those rocks in addition to returning the context images in which they were detected. Such approaches, coupled with appropriate navigational software, help to address both the data volume and command cycle bottlenecks that limit both rover mobility and science yield. We are developing fast, autonomous algorithms to enable such intelligent on-board decision making by spacecraft. Autonomous algorithms developed to date have the ability to identify rocks and layers in a scene, locate the horizon, and compress multi-spectral image data. Output from these algorithms could be used to autonomously obtain rock spectra, determine which images should be transmitted to the ground, or to aid in image compression. We will discuss these and other algorithms and demonstrate their performance during a recent rover field test.

Use of CCSDS Packets Over SpaceWire to Control Hardware

NASA Technical Reports Server (NTRS)

Haddad, Omar; Blau, Michael; Haghani, Noosha; Yuknis, William; Albaijes, Dennis

2012-01-01

For the Lunar Reconnaissance Orbiter, the Command and Data Handling subsystem consisted of several electronic hardware assemblies that were connected with SpaceWire serial links. Electronic hardware would be commanded/controlled and telemetry data was obtained using the SpaceWire links. Prior art focused on parallel data buses and other types of serial buses, which were not compatible with the SpaceWire and the core flight executive (CFE) software bus. This innovation applies to anything that utilizes both SpaceWire networks and the CFE software. The CCSDS (Consultative Committee for Space Data Systems) packet contains predetermined values in its payload fields that electronic hardware attached at the terminus of the SpaceWire node would decode, interpret, and execute. The hardware s interpretation of the packet data would enable the hardware to change its state/configuration (command) or generate status (telemetry). The primary purpose is to provide an interface that is compatible with the hardware and the CFE software bus. By specifying the format of the CCSDS packet, it is possible to specify how the resulting hardware is to be built (in terms of digital logic) that results in a hardware design that can be controlled by the CFE software bus in the final application

Real-time multisensor data fusion for target detection, classification, tracking, counting, and range estimates

NASA Astrophysics Data System (ADS)

Tsui, Eddy K.; Thomas, Russell L.

2004-09-01

As part of the Commanding General of Army Material Command's Research, Development & Engineering Command (RDECOM), the U.S. Army Research Development and Engineering Center (ARDEC), Picatinny funded a joint development effort with McQ Associates, Inc. to develop an Advanced Minefield Sensor (AMS) as a technology evaluation prototype for the Anti-Personnel Landmine Alternatives (APLA) Track III program. This effort laid the fundamental groundwork of smart sensors for detection and classification of targets, identification of combatant or noncombatant, target location and tracking at and between sensors, fusion of information across targets and sensors, and automatic situation awareness to the 1st responder. The efforts have culminated in developing a performance oriented architecture meeting the requirements of size, weight, and power (SWAP). The integrated digital signal processor (DSP) paradigm is capable of computing signals from sensor modalities to extract needed information within either a 360° or fixed field of view with acceptable false alarm rate. This paper discusses the challenges in the developments of such a sensor, focusing on achieving reasonable operating ranges, achieving low power, small size and low cost, and applications for extensions of this technology.

Using Unconstrained Tongue Motion as an Alternative Control Mechanism for Wheeled Mobility

PubMed Central

Huo, Xueliang; Ghovanloo, Maysam

2015-01-01

Tongue drive system (TDS) is a tongue-operated, minimally invasive, unobtrusive, noncontact, and wireless assistive technology that infers users’ intentions by detecting and classifying their voluntary tongue motions, and translating them to user-defined commands. We have developed customized interface circuitry between an external TDS (eTDS) prototype and a commercial powered wheelchair (PWC) as well as three control strategies to evaluate the tongue motion as an alternative control input for wheeled mobility. We tested the eTDS performance in driving PWCs on 12 able-bodied human subjects, of which 11 were novice. The results showed that all subjects could complete navigation tasks by operating the PWC using their tongue motions. Despite little prior experience, the average time using the eTDS and the tongue was only approximately three times longer than using a joystick and the fingers. Navigation time was strongly dependant on the number of issued commands, which reduced by gaining experience. Particularly, the unintended issued commands (the Midas touch problem) were rare, demonstrating the effectiveness of the tongue tracking and external magnetic field cancellation algorithms as well as the safety of the TDS for wheeled mobility. PMID:19362901

Using unconstrained tongue motion as an alternative control mechanism for wheeled mobility.

PubMed

Huo, Xueliang; Ghovanloo, Maysam

2009-06-01

Tongue drive system (TDS) is a tongue-operated, minimally invasive, unobtrusive, noncontact, and wireless assistive technology that infers users' intentions by detecting and classifying their voluntary tongue motions, and translating them to user-defined commands. We have developed customized interface circuitry between an external TDS (eTDS) prototype and a commercial powered wheelchair (PWC) as well as three control strategies to evaluate the tongue motion as an alternative control input for wheeled mobility. We tested the eTDS performance in driving PWCs on 12 able-bodied human subjects, of which 11 were novice. The results showed that all subjects could complete navigation tasks by operating the PWC using their tongue motions. Despite little prior experience, the average time using the eTDS and the tongue was only approximately three times longer than using a joystick and the fingers. Navigation time was strongly dependant on the number of issued commands, which reduced by gaining experience. Particularly, the unintended issued commands (the Midas touch problem) were rare, demonstrating the effectiveness of the tongue tracking and external magnetic field cancellation algorithms as well as the safety of the TDS for wheeled mobility.

Digital Camera Control for Faster Inspection

NASA Technical Reports Server (NTRS)

Brown, Katharine; Siekierski, James D.; Mangieri, Mark L.; Dekome, Kent; Cobarruvias, John; Piplani, Perry J.; Busa, Joel

2009-01-01

Digital Camera Control Software (DCCS) is a computer program for controlling a boom and a boom-mounted camera used to inspect the external surface of a space shuttle in orbit around the Earth. Running in a laptop computer in the space-shuttle crew cabin, DCCS commands integrated displays and controls. By means of a simple one-button command, a crewmember can view low- resolution images to quickly spot problem areas and can then cause a rapid transition to high- resolution images. The crewmember can command that camera settings apply to a specific small area of interest within the field of view of the camera so as to maximize image quality within that area. DCCS also provides critical high-resolution images to a ground screening team, which analyzes the images to assess damage (if any); in so doing, DCCS enables the team to clear initially suspect areas more quickly than would otherwise be possible and further saves time by minimizing the probability of re-imaging of areas already inspected. On the basis of experience with a previous version (2.0) of the software, the present version (3.0) incorporates a number of advanced imaging features that optimize crewmember capability and efficiency.

The SAS-3 delayed command system

NASA Technical Reports Server (NTRS)

Hoffman, E. J.

1975-01-01

To meet the requirements arising from the increased complexity of the power, attitude control and telemetry systems, a full redundant high-performance control section with delayed command capability was designed for the Small Astronomy Satellite-3 (SAS-3). The relay command system of SAS-3 is characterized by 56 bystate relay commands, with capability for handling up to 64 commands in future versions. The 'short' data command service of SAS-1 and SAS-2 consisting of shifting 24-bit words to two users was expanded to five users and augmented with a 'long load' data command service (up to 4080 bits) used to program the telemetry system and the delayed command subsystem. The inclusion of a delayed command service ensures a program of up to 30 relay or short data commands to be loaded for execution at designated times. The design and system operation of the SAS-3 command section are analyzed, with special attention given to the delayed command subsystem.

D-peaks: a visual tool to display ChIP-seq peaks along the genome.

PubMed

Brohée, Sylvain; Bontempi, Gianluca

2012-01-01

ChIP-sequencing is a method of choice to localize the positions of protein binding sites on DNA on a whole genomic scale. The deciphering of the sequencing data produced by this novel technique is challenging and it is achieved by their rigorous interpretation using dedicated tools and adapted visualization programs. Here, we present a bioinformatics tool (D-peaks) that adds several possibilities (including, user-friendliness, high-quality, relative position with respect to the genomic features) to the well-known visualization browsers or databases already existing. D-peaks is directly available through its web interface http://rsat.ulb.ac.be/dpeaks/ as well as a command line tool.

Sampling the potential energy surface of a DNA duplex damaged by a food carcinogen: Force field parameterization by ab initio quantum calculations and conformational searching using molecular mechanics computations

NASA Astrophysics Data System (ADS)

Wu, Xiangyang

1999-07-01

The heterocyclic amine 2-amino-3-methylimidazo (4, 5-f) quinoline (IQ) is one of a number of carcinogens found in barbecued meat and fish. It induces tumors in mammals and is probably involved in human carcinogenesis, because of great exposure to such food carcinogens. IQ is biochemically activated to a derivative which reacts with DNA to form a covalent adduct. This adduct may deform the DNA and consequently cause a mutation. which may initiate carcinogenesis. To understand this cancer initiating event, it is necessary to obtain atomic resolution structures of the damaged DNA. No such structures are available experimentally due to synthesis difficulties. Therefore, we employ extensive molecular mechanics and dynamics calculations for this purpose. The major IQ-DNA adduct in the specific DNA sequence d(5'G1G2C G3CCA3') - d(5'TGGCGCC3') with IQ modified at G3 is studied. The d(5'G1G2C G3CC3') sequence has recently been shown to be a hot-spot for mutations when IQ modification is at G3. Although this sequence is prone to -2 deletions via a ``slippage mechanism'' even when unmodified, a key question is why IQ increases the mutation frequency of the unmodified DNA by about 104 fold. Is there a structural feature imposed by IQ that is responsible? The molecular mechanics and dynamics program AMBER for nucleic acids with the latest force field was chosen for this work. This force field has been demonstrated to reproduce well the B-DNA structure. However, some parameters, the partial charges, bond lengths and angles, dihedral parameters of the modified residue, are not available in the AMBER database. We parameterized the force field using high level ab initio quantum calculations. We created 800 starting conformations which uniformly sampled in combination at 18° intervals three torsion angles that govern the IQ-DNA orientations, and energy minimized them. The most important structures are abnormal; the IQ damaged guanine is rotated out of its standard B-DNA orientations, compromising its ability to act as a faithful template during DNA replication.

14 CFR 91.1031 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Pilot in command or second in command... RULES Fractional Ownership Operations Program Management § 91.1031 Pilot in command or second in command: Designation required. (a) Each program manager must designate a— (1) Pilot in command for each program flight...

32 CFR 724.405 - Commandant of the Marine Corps or the Commander, Naval Military Personnel Command.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 5 2014-07-01 2014-07-01 false Commandant of the Marine Corps or the Commander, Naval Military Personnel Command. 724.405 Section 724.405 National Defense Department of Defense... Personnel Command. Personnel managers of the Marine Corps and the Navy; responsible for providing limited...

32 CFR 700.1058 - Command of a submarine.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false Command of a submarine. 700.1058 Section 700... Command Detail to Duty § 700.1058 Command of a submarine. The officer detailed to command a submarine shall be an officer of the line in the Navy, eligible for command at sea and qualified for command of...

14 CFR 135.109 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Pilot in command or second in command... ON BOARD SUCH AIRCRAFT Flight Operations § 135.109 Pilot in command or second in command: Designation required. (a) Each certificate holder shall designate a— (1) Pilot in command for each flight; and (2...

32 CFR 724.405 - Commandant of the Marine Corps or the Commander, Naval Military Personnel Command.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false Commandant of the Marine Corps or the Commander, Naval Military Personnel Command. 724.405 Section 724.405 National Defense Department of Defense... Personnel Command. Personnel managers of the Marine Corps and the Navy; responsible for providing limited...

14 CFR 1214.703 - Chain of command.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Chain of command. 1214.703 Section 1214.703... Shuttle Commander § 1214.703 Chain of command. (a) The Commander is a career NASA astronaut who has been... particular flight and is second in command of the flight. If the commander is unable to carry out the...

14 CFR 135.109 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Pilot in command or second in command... ON BOARD SUCH AIRCRAFT Flight Operations § 135.109 Pilot in command or second in command: Designation required. (a) Each certificate holder shall designate a— (1) Pilot in command for each flight; and (2...

32 CFR 724.405 - Commandant of the Marine Corps or the Commander, Naval Military Personnel Command.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 5 2011-07-01 2011-07-01 false Commandant of the Marine Corps or the Commander, Naval Military Personnel Command. 724.405 Section 724.405 National Defense Department of Defense... Personnel Command. Personnel managers of the Marine Corps and the Navy; responsible for providing limited...

32 CFR 724.405 - Commandant of the Marine Corps or the Commander, Naval Military Personnel Command.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false Commandant of the Marine Corps or the Commander, Naval Military Personnel Command. 724.405 Section 724.405 National Defense Department of Defense... Personnel Command. Personnel managers of the Marine Corps and the Navy; responsible for providing limited...

14 CFR 91.1031 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Pilot in command or second in command... RULES Fractional Ownership Operations Program Management § 91.1031 Pilot in command or second in command: Designation required. (a) Each program manager must designate a— (1) Pilot in command for each program flight...

32 CFR 700.1058 - Command of a submarine.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false Command of a submarine. 700.1058 Section 700... Command Detail to Duty § 700.1058 Command of a submarine. The officer detailed to command a submarine shall be an officer of the line in the Navy, eligible for command at sea and qualified for command of...

14 CFR 1214.703 - Chain of command.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Chain of command. 1214.703 Section 1214.703... Shuttle Commander § 1214.703 Chain of command. (a) The Commander is a career NASA astronaut who has been... particular flight and is second in command of the flight. If the commander is unable to carry out the...

14 CFR 91.1031 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Pilot in command or second in command... RULES Fractional Ownership Operations Program Management § 91.1031 Pilot in command or second in command: Designation required. (a) Each program manager must designate a— (1) Pilot in command for each program flight...

32 CFR 700.1058 - Command of a submarine.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Command of a submarine. 700.1058 Section 700... Command Detail to Duty § 700.1058 Command of a submarine. The officer detailed to command a submarine shall be an officer of the line in the Navy, eligible for command at sea and qualified for command of...

32 CFR 700.1058 - Command of a submarine.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 5 2014-07-01 2014-07-01 false Command of a submarine. 700.1058 Section 700... Command Detail to Duty § 700.1058 Command of a submarine. The officer detailed to command a submarine shall be an officer of the line in the Navy, eligible for command at sea and qualified for command of...

14 CFR 135.109 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Pilot in command or second in command... ON BOARD SUCH AIRCRAFT Flight Operations § 135.109 Pilot in command or second in command: Designation required. (a) Each certificate holder shall designate a— (1) Pilot in command for each flight; and (2...

14 CFR 135.109 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Pilot in command or second in command... ON BOARD SUCH AIRCRAFT Flight Operations § 135.109 Pilot in command or second in command: Designation required. (a) Each certificate holder shall designate a— (1) Pilot in command for each flight; and (2...

32 CFR 700.1058 - Command of a submarine.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 5 2013-07-01 2013-07-01 false Command of a submarine. 700.1058 Section 700... Command Detail to Duty § 700.1058 Command of a submarine. The officer detailed to command a submarine shall be an officer of the line in the Navy, eligible for command at sea and qualified for command of...

32 CFR 724.405 - Commandant of the Marine Corps or the Commander, Naval Military Personnel Command.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 5 2012-07-01 2012-07-01 false Commandant of the Marine Corps or the Commander, Naval Military Personnel Command. 724.405 Section 724.405 National Defense Department of Defense... Personnel Command. Personnel managers of the Marine Corps and the Navy; responsible for providing limited...

14 CFR 1214.703 - Chain of command.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Chain of command. 1214.703 Section 1214.703... Shuttle Commander § 1214.703 Chain of command. (a) The Commander is a career NASA astronaut who has been... particular flight and is second in command of the flight. If the commander is unable to carry out the...

14 CFR 1214.703 - Chain of command.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Chain of command. 1214.703 Section 1214.703... Shuttle Commander § 1214.703 Chain of command. (a) The Commander is a career NASA astronaut who has been... particular flight and is second in command of the flight. If the commander is unable to carry out the...

14 CFR 135.109 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Pilot in command or second in command... ON BOARD SUCH AIRCRAFT Flight Operations § 135.109 Pilot in command or second in command: Designation required. (a) Each certificate holder shall designate a— (1) Pilot in command for each flight; and (2...

14 CFR 91.1031 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Pilot in command or second in command... RULES Fractional Ownership Operations Program Management § 91.1031 Pilot in command or second in command: Designation required. (a) Each program manager must designate a— (1) Pilot in command for each program flight...

14 CFR 91.1031 - Pilot in command or second in command: Designation required.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Pilot in command or second in command... RULES Fractional Ownership Operations Program Management § 91.1031 Pilot in command or second in command: Designation required. (a) Each program manager must designate a— (1) Pilot in command for each program flight...

A comparison of sedimentary DNA and pollen from lake sediments in recording vegetation composition at the Siberian treeline.

PubMed

Niemeyer, Bastian; Epp, Laura S; Stoof-Leichsenring, Kathleen R; Pestryakova, Luidmila A; Herzschuh, Ulrike

2017-11-01

Reliable information on past and present vegetation is important to project future changes, especially for rapidly transitioning areas such as the boreal treeline. To study past vegetation, pollen analysis is common, while current vegetation is usually assessed by field surveys. Application of detailed sedimentary DNA (sedDNA) records has the potential to enhance our understanding of vegetation changes, but studies systematically investigating the power of this proxy are rare to date. This study compares sedDNA metabarcoding and pollen records from surface sediments of 31 lakes along a north-south gradient of increasing forest cover in northern Siberia (Taymyr peninsula) with data from field surveys in the surroundings of the lakes. sedDNA metabarcoding recorded 114 plant taxa, about half of them to species level, while pollen analyses identified 43 taxa, both exceeding the 31 taxa found by vegetation field surveys. Increasing Larix percentages from north to south were consistently recorded by all three methods and principal component analyses based on percentage data of vegetation surveys and DNA sequences separated tundra from forested sites. Comparisons of the ordinations using procrustes and protest analyses show a significant fit among all compared pairs of records. Despite similarities of sedDNA and pollen records, certain idiosyncrasies, such as high percentages of Alnus and Betula in all pollen and high percentages of Salix in all sedDNA spectra, are observable. Our results from the tundra to single-tree tundra transition zone show that sedDNA analyses perform better than pollen in recording site-specific richness (i.e., presence/absence of taxa in the vicinity of the lake) and perform as well as pollen in tracing vegetation composition. © 2017 John Wiley & Sons Ltd.

KSC-03pd1109

NASA Image and Video Library

2003-04-10

KENNEDY SPACE CENTER, FLA. -- A memorial erected by the Hemphill, Texas, community commemorates a location where remains of a fallen Columbia astronaut were found. Kennedy Space Center workers are participating in the Columbia Recovery efforts at the Lufkin (Texas) Command Center, four field sites in East Texas, and the Barksdale, La., hangar site. KSC is working with representatives from other NASA Centers and with those from a number of federal, state and local agencies in the recovery effort. KSC provides vehicle technical expertise in the field to identify, collect and return Shuttle hardware to KSC.

Human Factors Design Guidelines for the Army Tactical Command and Control System (ATTCS) Soldier-Machine Interface. Version 2.0

DTIC Science & Technology

1992-05-01

especially true for friend-enemy or danger-safe designations. Dots, dashes, shapes, and video effects are recommended. Care must be taken to avoid visual...MAY 92 10.3 Screen Design - Format 10.3.1.4 Use of Contrasting Features Use contrasting features such as inverse video and color to call attention to...captions. Do not use reverse video or highlighting for labels. 13.2.3.2 Formatting For single fields, locate the caption to the left of the entry fields

Field testing of Schistosoma japonicum DNA vaccines in cattle in China.

PubMed

Shi, Fuhui; Zhang, Yaobi; Lin, Jiaojiao; Zuo, Xin; Shen, Wei; Cai, Yiumin; Ye, Ping; Bickle, Quentin D; Taylor, Martin G

2002-11-01

Vaccines are needed to reduce the zoonotic reservoir of Schistosoma japonicum infection in bovines in China. We have developed two experimental DNA vaccines and have already shown these to be capable of inducing partial protection in water buffalo naturally exposed to the risk of S. japonicum infection in the field. We now report a similar field trial in cattle, the other major bovine reservoir host species in China. Groups of cattle were vaccinated with the VRSj28 vaccine or the VRSj23 vaccine, or, to test whether protection could be enhanced by combination vaccination, with both these DNA vaccines together. After vaccination, the cattle were exposed to natural infection in the field for a period of 54 days. Worm and egg counts carried out at the end of the experiment showed that each of the vaccine groups showed partial resistance, and that combined vaccination was not more effective than vaccination with the individual plasmids.

Improved Force Fields for Peptide Nucleic Acids with Optimized Backbone Torsion Parameters.

PubMed

Jasiński, Maciej; Feig, Michael; Trylska, Joanna

2018-06-06

Peptide nucleic acids are promising nucleic acid analogs for antisense therapies as they can form stable duplex and triplex structures with DNA and RNA. Computational studies of PNA-containing duplexes and triplexes are an important component for guiding their design, yet existing force fields have not been well validated and parametrized with modern computational capabilities. We present updated CHARMM and Amber force fields for PNA that greatly improve the stability of simulated PNA-containing duplexes and triplexes in comparison with experimental structures and allow such systems to be studied on microsecond time scales. The force field modifications focus on reparametrized PNA backbone torsion angles to match high-level quantum mechanics reference energies for a model compound. The microsecond simulations of PNA-PNA, PNA-DNA, PNA-RNA, and PNA-DNA-PNA complexes also allowed a comprehensive analysis of hydration and ion interactions with such systems.

Re-engineering the Multimission Command System at the Jet Propulsion Laboratory

NASA Technical Reports Server (NTRS)

Alexander, Scott; Biesiadecki, Jeff; Cox, Nagin; Murphy, Susan C.; Reeve, Tim

1994-01-01

The Operations Engineering Lab (OEL) at JPL has developed the multimission command system as part of JPL's Advanced Multimission Operations System. The command system provides an advanced multimission environment for secure, concurrent commanding of multiple spacecraft. The command functions include real-time command generation, command translation and radiation, status reporting, some remote control of Deep Space Network antenna functions, and command file management. The mission-independent architecture has allowed easy adaptation to new flight projects and the system currently supports all JPL planetary missions (Voyager, Galileo, Magellan, Ulysses, Mars Pathfinder, and CASSINI). This paper will discuss the design and implementation of the command software, especially trade-offs and lessons learned from practical operational use. The lessons learned have resulted in a re-engineering of the command system, especially in its user interface and new automation capabilities. The redesign has allowed streamlining of command operations with significant improvements in productivity and ease of use. In addition, the new system has provided a command capability that works equally well for real-time operations and within a spacecraft testbed. This paper will also discuss new development work including a multimission command database toolkit, a universal command translator for sequencing and real-time commands, and incorporation of telecommand capabilities for new missions.

Coupling Spore Traps and Quantitative PCR Assays for Detection of the Downy Mildew Pathogens of Spinach (Peronospora effusa) and Beet (P. schachtii)

PubMed Central

Klosterman, Steven J.; Anchieta, Amy; McRoberts, Neil; Koike, Steven T.; Subbarao, Krishna V.; Voglmayr, Hermann; Choi, Young-Joon; Thines, Marco; Martin, Frank N.

2016-01-01

Downy mildew of spinach (Spinacia oleracea), caused by Peronospora effusa, is a production constraint on production worldwide, including in California, where the majority of U.S. spinach is grown. The aim of this study was to develop a real-time quantitative polymerase chain reaction (qPCR) assay for detection of airborne inoculum of P. effusa in California. Among oomycete ribosomal DNA (rDNA) sequences examined for assay development, the highest nucleotide sequence identity was observed between rDNA sequences of P. effusa and P. schachtii, the cause of downy mildew on sugar beet and Swiss chard in the leaf beet group (Beta vulgaris subsp. vulgaris). Single-nucleotide polymorphisms were detected between P. effusa and P. schachtii in the 18S rDNA regions for design of P. effusa- and P. schachtii-specific TaqMan probes and reverse primers. An allele-specific probe and primer amplification method was applied to determine the frequency of both P. effusa and P. schachtii rDNA target sequences in pooled DNA samples, enabling quantification of rDNA of P. effusa from impaction spore trap samples collected from spinach production fields. The rDNA copy numbers of P. effusa were, on average, ≈3,300-fold higher from trap samples collected near an infected field compared with those levels recorded at a site without a nearby spinach field. In combination with disease-conducive weather forecasting, application of the assays may be helpful to time fungicide applications for disease management. PMID:24964150