Sample records for floating drilling rig

  1. 33 CFR 155.400 - Platform machinery space drainage on oceangoing fixed and floating drilling rigs and other...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION OIL OR HAZARDOUS MATERIAL POLLUTION PREVENTION REGULATIONS FOR VESSELS Vessel Equipment § 155.400 Platform...

  2. 33 CFR 155.400 - Platform machinery space drainage on oceangoing fixed and floating drilling rigs and other...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) Complies with the oily-water separating equipment requirements of a valid National Pollutant Discharge... Chapter I; (2) Complies with the oily-water separating equipment requirements for oceangoing ships of 400... installed bilge pumping system for discharge of oily mixtures from platform machinery spaces into the sea...

  3. 33 CFR 155.400 - Platform machinery space drainage on oceangoing fixed and floating drilling rigs and other...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) Complies with the oily-water separating equipment requirements of a valid National Pollutant Discharge... Chapter I; (2) Complies with the oily-water separating equipment requirements for oceangoing ships of 400... installed bilge pumping system for discharge of oily mixtures from platform machinery spaces into the sea...

  4. 33 CFR 155.400 - Platform machinery space drainage on oceangoing fixed and floating drilling rigs and other...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) Complies with the oily-water separating equipment requirements of a valid National Pollutant Discharge... Chapter I; (2) Complies with the oily-water separating equipment requirements for oceangoing ships of 400... installed bilge pumping system for discharge of oily mixtures from platform machinery spaces into the sea...

  5. 33 CFR 155.400 - Platform machinery space drainage on oceangoing fixed and floating drilling rigs and other...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) Complies with the oily-water separating equipment requirements of a valid National Pollutant Discharge... Chapter I; (2) Complies with the oily-water separating equipment requirements for oceangoing ships of 400... installed bilge pumping system for discharge of oily mixtures from platform machinery spaces into the sea...

  6. 33 CFR 151.25 - Oil Record Book.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Oil Record Book. 151.25 Section... Treaty as it Pertains to Pollution from Ships Oil Pollution § 151.25 Oil Record Book. (a) Each oil tanker... fixed or floating drilling rig or other platform shall maintain an Oil Record Book Part I (Machinery...

  7. 33 CFR 151.25 - Oil Record Book.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Oil Record Book. 151.25 Section... Treaty as it Pertains to Pollution from Ships Oil Pollution § 151.25 Oil Record Book. (a) Each oil tanker... fixed or floating drilling rig or other platform shall maintain an Oil Record Book Part I (Machinery...

  8. 33 CFR 151.25 - Oil Record Book.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Oil Record Book. 151.25 Section... Treaty as it Pertains to Pollution from Ships Oil Pollution § 151.25 Oil Record Book. (a) Each oil tanker... fixed or floating drilling rig or other platform shall maintain an Oil Record Book Part I (Machinery...

  9. 33 CFR 151.25 - Oil Record Book.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Oil Record Book. 151.25 Section... Treaty as it Pertains to Pollution from Ships Oil Pollution § 151.25 Oil Record Book. (a) Each oil tanker... fixed or floating drilling rig or other platform shall maintain an Oil Record Book Part I (Machinery...

  10. Garden Banks 388 subsea drilling/production template: Project management of a fast-track project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ledbetter, W.R.

    1995-10-01

    Enserch Exploration`s Garden Banks 388 development is a production scheme based around a floating drilling and production facility and subsea drilling/production template. The Floating Production Facility (FPF) is a converted semisubmersible drilling rig which will drill and product through a 24-well slot template. This development is located in Block 388 of the Garden Banks area in the Gulf of Mexico approximately 200 miles southwest of New Orleans. Louisiana. This production system is being installed in an area of known oil and gas reserves and will produce to a shallow water platform 54 miles away at Ewing Bank 315. The FPFmore » will be permanently moored on the surface above the template. The subsea template has been installed in 2,190 feet of water and will produce through a 2,000 foot free-standing production riser system to the FPF. The produced fluids are partially separated on the FPF before oil and gas are pumped through the template to export gathering lines which are connected to the shallow water facility.« less

  11. Design, fabrication, and integration testing of the Garden Banks 388 subsea production template

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ledbetter, W.R.; Smith, D.W.; Pierce, D.M.

    1995-12-31

    Enserch Exploration`s Garden Banks 388 development has a production scheme based around a floating drilling and production facility and subsea drilling/production template. The Floating Production Facility (FPF) is a converted semisubmersible drilling rig that will drill and produce through a 24-well slot subsea template. This development is located in Block 388 of the Garden Banks area in the Gulf of Mexico approximately 200 miles southwest of New Orleans, Louisiana. The production system is being installed in an area of known oil and gas reserves and will produce to a shallow water platform 54 miles away at Eugene Island 315. Themore » FPF will be permanently moored above the template. The subsea template has been installed in 2190 feet of water and will produce through a 2,000 foot free-standing production riser system to the FPF. The produced fluids are partially separated on the FPF before oil and gas are pumped through the template to export gathering lines that are connected to the shallow water facility. The system designed through-put is 40,000 BOPD of oil and 120 MMSCFD of gas.« less

  12. Lapland longspur mortality at an oil well drilling rig site, Laramie County, Wyoming

    USGS Publications Warehouse

    Ramirez, Pedro; Dickerson, Kimberly K.; Lindstrom, Jim; Meteyer, Carol U.; Darrah, Scott

    2015-01-01

    Two hundred fifty-one Lapland longspur (Calcarius lapponicus) carcasses were recovered around an oil well drilling rig in Laramie County, Wyoming, USA, on December 13–14, 2010, apparent victims of a winter storm and “light entrapment” from the lights on the drilling rig during foggy conditions. We found Lapland longspur carcasses distributed around the drilling rig from 33 m to 171 m. Investigators did not find evidence of bird carcasses on the drilling rig deck or equipment immediately adjacent to the drilling rig. We ruled out chemical toxins and disease as a cause of mortality. Weather conditions, the circular depositional pattern of carcasses around the drilling rig, and bird necropsy results led investigators to conclude that the Lapland longspur mortality was the result of the migrating birds entering the area illuminated by the drilling rig lights in freezing fog and the birds repeatedly circling the drilling rig until they fell to the ground in exhaustion and dying from subsequent trauma. Further research is needed to understand how to most effectively adjust lighting of onshore drilling rigs to reduce the potential for avian light entrapment. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.

  13. A Universal Rig for Supporting Large Hammer Drills: Reduced Injury Risk and Improved Productivity

    PubMed Central

    Rempel, David; Barr, Alan

    2015-01-01

    Drilling holes into concrete with heavy hammer and rock drills is one of the most physically demanding tasks performed in commercial construction and poses risks for musculoskeletal disorders, noise induced hearing loss, hand arm vibration syndrome and silicosis. The aim of this study was to (1) use a participatory process to develop a rig to support pneumatic rock drills or large electric hammer drills in order to reduce the health risks and (2) evaluate the usability of the rig. Seven prototype rigs for supporting large hammer drills were developed and modified with feedback from commercial contractors and construction workers. The final design was evaluated by laborers and electricians (N=29) who performed their usual concrete drilling with the usual method and the new rig. Subjective regional fatigue was significantly less in the neck, shoulders, hands and arms, and lower back) when using the universal rig compared to the usual manual method. Usability ratings for the rig were significantly better than the usual method on stability, control, drilling, accuracy, and vibration. Drilling time was reduced by approximately 50% with the rig. Commercial construction contractors, laborers and electricians who use large hammer drills for drilling many holes should consider using such a rig to prevent musculoskeletal disorders, fatigue, and silicosis. PMID:26005290

  14. Advantages and limitations of remotely operated sea floor drill rigs

    NASA Astrophysics Data System (ADS)

    Freudenthal, T.; Smith, D. J.; Wefer, G.

    2009-04-01

    A variety of research targets in marine sciences including the investigation of gas hydrates, slope stability, alteration of oceanic crust, ore formation and palaeoclimate can be addressed by shallow drilling. However, drill ships are mostly used for deep drillings, both because the effort of building up a drill string from a drill ship to the deep sea floor is tremendous and control on drill bit pressure from a movable platform and a vibrating drill string is poor especially in the upper hundred meters. During the last decade a variety of remotely operated drill rigs have been developed, that are deployed on the sea bed and operated from standard research vessels. These developments include the BMS (Bentic Multicoring System, developed by Williamson and Associates, operated by the Japanese Mining Agency), the PROD (Portable Remotely Operated Drill, developed and operated by Benthic Geotech), the Rockdrill 2 (developed and operated by the British geological Survey) and the MeBo (German abbreviation for sea floor drill rig, developed and operated by Marum, University of Bremen). These drill rigs reach drilling depths between 15 and 100 m. For shallow drillings remotely operated drill rigs are a cost effective alternative to the services of drill ships and have the major advantage that the drilling operations are performed from a stable platform independent of any ship movements due to waves, wind or currents. Sea floor drill rigs can be deployed both in shallow waters and the deep sea. A careful site survey is required before deploying the sea floor drill rig. Slope gradient, small scale topography and soil strength are important factors when planning the deployment. The choice of drill bits and core catcher depend on the expected geology. The required drill tools are stored on one or two magazines on the drill rig. The MeBo is the only remotely operated drill rig world wide that can use wire line coring technique. This method is much faster than conventional drilling. It has the advantage that the drill string stays in the drilled hole during the entire drilling process and prevents the drilled hole from collapsing while the inner core barrels comprising the drilled core sections are hooked up inside the drill string using a wire.

  15. Increasing Geothermal Energy Demand: The Need for Urbanization of the Drilling Industry

    ERIC Educational Resources Information Center

    Teodoriu, Catalin; Falcone, Gioia

    2008-01-01

    Drilling wells in urban spaces requires special types of rigs that do not conflict with the surrounding environment. For this, a mutation of the current drilling equipment is necessary into what can be defined as an "urbanized drilling rig." Noise reduction, small footprint, and "good looking" rigs all help persuade the general public to accept…

  16. Analysis and design of trial well mooring in deepwater of the South China Sea

    NASA Astrophysics Data System (ADS)

    Guo, Yongfeng; Ji, Shaojun; Tang, Changquan; Li, Jiansong; Zhong, Huiquan; Ian, Ong Chin Yam

    2012-06-01

    Mooring systems play an important role for semi-submersible rigs that drill in deepwater. A detailed analysis was carried out on the mooring of a semi-submersible rig that conducted a trial well drilling at a deepwater location in the South China Sea in 2009. The rig was 30 years old and had a shallow platform with a designed maximum operating water depth of 457 m. Following the mooring analysis, a mooring design was given that requires upgrading of the rig's original mooring system. The upgrade included several innovations, such as installing eight larger anchors, i.e. replacing the original anchors and inserting an additional 600 m of steel wires with the existing chains. All this was done to enhance the mooring capability of the rig in order for the rig to be held in position to conduct drilling at a water depth of 476 m. The overall duration of the drilling was 50 days and the upgraded mooring system proved to be efficient in achieving the goal of keeping the rig stationary while it was drilling the trial well in the South China Sea. This successful campaign demonstrates that an older semi-submersible rig can take on drilling in deep water after careful design and proper upgrading and modification to the original mooring system.

  17. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mehra, S.; Bryce, T.

    Sedco Forex has recently completed a new land drilling rig, currently deployed in Gabon, that integrates well construction activities with multiskilling to create cost savings across the board in drilling operations. Historically, operators have produced a comprehensive tender package specifying strictly the type and size of individual rig components and the number of personnel required to drill. In this case, the drilling contractor provides a fit-for-purpose rig, consistent with field location, well profile, operator`s priorities, and local constraints.

  18. Injury Rates on New and Old Technology Oil and Gas Rigs Operated by the Largest United States Onshore Drilling Contractor

    PubMed Central

    Blackley, David J.; Retzer, Kyla D.; Hubler, Warren G.; Hill, Ryan D.; Laney, A. Scott

    2015-01-01

    Background Occupational fatality rates among oil and gas extraction industry and specifically among drilling contractor workers are high compared to the U.S. all-industry average. There is scant literature focused on non-fatal injuries among drilling contractors, some of which have introduced engineering controls to improve rig efficiency and reduce injury risk. Methods We compared injury rates on new and old technology rigs operated by the largest U.S. drilling contractor during 2003–2012, stratifying by job type and grouping outcomes by injury severity and body part affected. Results Six hundred seventy-one injuries were recorded over 77.4 million person-hours. The rate on new rigs was 66% of that on old rigs. Roughnecks had lower injury rates on new rigs, largely through reduced limb injury rates. New rigs had lower rates in each non-fatal injury severity category. Conclusions For this company, new technology rigs appear to provide a safer environment for roughnecks. Future studies could include data from additional companies. PMID:25164118

  19. Injury rates on new and old technology oil and gas rigs operated by the largest United States onshore drilling contractor.

    PubMed

    Blackley, David J; Retzer, Kyla D; Hubler, Warren G; Hill, Ryan D; Laney, A Scott

    2014-10-01

    Occupational fatality rates among oil and gas extraction industry and specifically among drilling contractor workers are high compared to the U.S. all-industry average. There is scant literature focused on non-fatal injuries among drilling contractors, some of which have introduced engineering controls to improve rig efficiency and reduce injury risk. We compared injury rates on new and old technology rigs operated by the largest U.S. drilling contractor during 2003-2012, stratifying by job type and grouping outcomes by injury severity and body part affected. Six hundred seventy-one injuries were recorded over 77.4 million person-hours. The rate on new rigs was 66% of that on old rigs. Roughnecks had lower injury rates on new rigs, largely through reduced limb injury rates. New rigs had lower rates in each non-fatal injury severity category. For this company, new technology rigs appear to provide a safer environment for roughnecks. Future studies could include data from additional companies. © 2014 Wiley Periodicals, Inc.

  20. New drilling rigs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tubb, M.

    1981-03-01

    Rig builders maintain their frantic work pace to meet drilling contractors' orders for new mobile units - principally the jack-up and semis now so popular. Leading new rig client is Santa Fe Drilling, which has ordered its seventh new offshore unit. The order includes two Enhanced 9500 Pacesetter Series semis, each scheduled to cost $80 million, to be built by Daewoo Shipbuilding and Heavy Machinery in Korea.

  1. New drilling rigs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tubb, M.

    1981-02-01

    Friede and Goldman Ltd. of New Orleans, Louisiana has a successful drilling rig, the L-780 jack-up series. The triangular-shaped drilling vessel measures 180 x 176 ft. and is equipped with three 352 ft legs including spud cans. It is designed to work in up to 250 ft waters and drill to 20,000 ft depths. The unit is scheduled to begin initial drilling operations in the Gulf of Mexico for Arco. Design features are included for the unit. Davie Shipbuilding Ltd. has entered the Mexican offshore market with the signing of a $40,000,000 Canadian contract for a jack-up to work inmore » 300 ft water depths. Baker Marine Corporation has contracted with the People's Republic of China for construction of two self-elevating jack-ups. The units will be built for Magnum Marine, headquartered in Houston. Details for the two rigs are given. Santa Fe International Corporation has ordered a new jack-up rig to work initially in the Gulf of Suez. The newly ordered unit, Rig 136, will be the company's fourth offshore drilling rig now being built in the Far East. Temple Drilling Company has signed a construction contract with Bethlehem Steel for a jack-up to work in 200 ft water depths. Penrod Drilling Company has ordered two additional cantilever type jack-ups for Hitachi Shipbuilding and Engineering Co. Ltd. of Japan. Two semi-submersibles, capable of working in up to 2000 ft water depths, have been ordered by two Liberian companies. Details for these rigs are included. (DP)« less

  2. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Huttrer, G.W.

    This report summarizes the investigation and evaluation of several {open_quotes}compact{close_quotes} drill rigs which could be used for drilling geothermal production wells. Use of these smaller rigs would save money by reducing mobilization costs, fuel consumption, crew sizes, and environmental impact. Advantages and disadvantages of currently-manufactured rigs are identified, and desirable characteristics for the {open_quotes}ideal{close_quotes} compact rig are defined. The report includes a detailed cost estimate of a specific rig, and an evaluation of the cost/benefit ratio of using this rig. Industry contacts for further information are given.

  3. Drilling Productivity Report

    EIA Publications

    2017-01-01

    Energy Information Administration’s (EIA) new Drilling Productivity Report (DPR) takes a fresh look at oil and natural gas production, starting with an assessment of how and where drilling for hydrocarbons is taking place. The DPR uses recent data on the total number of drilling rigs in operation along with estimates of drilling productivity and estimated changes in production from existing oil and natural gas wells to provide estimated changes in oil and natural gas production for six key fields. EIA's approach does not distinguish between oil-directed rigs and gas-directed rigs because once a well is completed it may produce both oil and gas; more than half of the wells produce both.

  4. Gulf of Mexico and Caribbean Sea Data and Model Base Report

    DTIC Science & Technology

    1979-07-01

    The source levels and spectral characteristics of merchant ships, drill rigs, and seismic profiling sources are reason- ably well known. Lacking...better data, fishing vessels are assumed to be 10 dB quieter than merchar• ships; production platforms are assumed to be similar to drill rigs, corrected...scope of the problem presented by production platforms, mobile drill rigs, and seismic profilers. 5. Impact on Exercise Planning Offshore oil industry

  5. The installation of a sub sea floor observatory using the sea floor drill rig MeBo

    NASA Astrophysics Data System (ADS)

    Wefer, G.; Freudenthal, T.; Kopf, A.

    2012-04-01

    Sea floor drill rigs that can be deployed from standard research vessels are bridging the gap between dedicated drill ships that are used for deep drillings in the range of several hundred meters below sea floor and conventional sampling tools like gravity corers, piston corer or dredges that only scratch the surface of the sea floor. A major advantage of such robotic drill rigs is that the drilling action is conducted from a stable platform at the sea bed independent of any ship movements due to waves, wind or currents. At the MARUM Center for Marine Environmental Sciences at the University of Bremen we developed the sea bed drill rig MeBo that can be deployed from standard research vessels. The drill rig is deployed on the sea floor and controlled from the vessel. Drilling tools for coring the sea floor down to 70 m can be stored on two magazines on the rig. A steel-armoured umbilical is used for lowering the rig to the sea bed in water depths up to 2000 m in the present system configuration. It was successfully operated on ten expeditions since 2005 and drilled more than 1000 m in different types of geology including hemipelagic mud, glacial till as well as sedimentary and crystalline rocks. MeBo boreholes be equipped with sensors and used for long term monitoring are planned. Depending on the scientific demands, a MeBoCORK monitoring system will allow in situ measurements of eg. temperature and pressure. The "MeBoCORK" will be equipped with data loggers and data transmission interface for reading out the collected data from the vessel. By additional payload installation on the MeBoCORK with an ROV it will be possible to increase the energy capacity as well as to conduct fluid sampling in the bore hole for geochemical analyses. It is planned to install a prototype of this additional payload with the MARUM ROV QUEST4000M during the following R/V SONNE cruise in July 2012.

  6. Innovative workover/drilling rigs to utilize hydraulics

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Noran, D.

    1975-09-29

    While Western Gear Corp., Everett, Wash., is currently building 2 models of a hydraulic workover/drilling rig (one offshore and the other a trailer-mounted land rig), Bender Co., Bakersfield, Calif., already has an all-hydraulic servicing/drilling rig undergoing tests. The rigs are similar in that they eliminate the traveling block, crown block, draw works, chains, and sprockets found on the conventional rig. The major design innovation on the Western Gear model is the 260,000-lb-capacity hoisting system in which 2 hydraulic rams are anchored to the rig floor and carry all the pipe weight, thus eliminating the danger of the derrick's being pulledmore » in. Other changes involve the tripping system, a power swivel/elevator, and the control valves. Maintenance and labor cost reductions are expected to be substantial. The Bender Co. rig has a single-section mast that is a lever-lift-type derrick which serves as a guide for the rams and a support for the pipe-racking platform. Hoisting capacity depends on the number and size of the lifting rods (which support the crown sheaves) and the hydraulic pressure applied. Manufacturing and operating costs are expected to be less than for conventional rigs.« less

  7. Minimum area rig concept update: H and P 101 modifications and first infield move

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sigurdson, S.R.

    1987-03-01

    The minimum area rig concept (MARC) is a cost-effective alternative to the typical self-contained platform rig (SCPR). Helmerich and Payne (HandP) built the first MARC rig, HandP 101, to drill and to work over wells up to 16,000 ft (4877 m) measured depth. This rig began operation in May 1983 in the Gulf of Mexico at Arco Oil and Gas Co.'s South Pass Block 61 field and has undergone one infield move. Since the rig's initial mobilization, several rig modifications have been added to increase storage area, to promote safety, to provide a more efficient drilling/workover rig, and to reducemore » overall move time. This paper describes the modifications and recaps the rig's first move. This provides further insight into the MARC rig and show the benefits of the MARC design in relation to a move.« less

  8. Cold resistant nickel-alloy steel

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Legostaev, Yu.L.; Karchevskaya, N.I.; Karchevnikov, V.P.

    1988-05-01

    Low-alloy cold-resistant steel 10GNB was developed for the construction of ships and floating drill rigs. The optimal heat-treatment regime for the steel was refinement. Reducing the carbon content improved its weldability and toughness properties. Optical metallography and electron microscopy established that the optimal structure was a tempered martensitic-bainitic mixture with uniformly distributed particles of disperse special niobium carbides NbC. The substructure and the processes of carbide and carbonitride phase segregation were studied by transmission and extraction electron microscopy. In mechanical tests the steel exhibited high resistance to brittle failure. In terms of corrosion resistance the steel corresponds to the requirementsmore » set forth for shipbuilding steels.« less

  9. Non-technical skills: enhancing safety in operating theatres (and drilling rigs).

    PubMed

    Flin, Rhona

    2014-03-01

    On April 20th 2010, a large Transocean drilling rig called the Deepwater Horizon was operating in the Gulf of Mexico to drill the Macondo well, for the oil company BP. The job was six weeks behind schedule and $58 million over budget and had not been without difficulty: it was a high pressure well, 2.5 miles below the seabed. At 5.45 am, the Halliburton cementing engineer sent an email to say: 'We have completed the job and it went well'. At 9.43 pm, 16 hours later, there was a release of hydrocarbons into the well bore and the drilling rig experienced a catastrophic blowout as the high pressure oil and gas escaped onto the rig and into the ocean. The resulting explosions and fire killed 11 of the crew of 126, injured many more and created an enormous oil spill across the Gulf.

  10. 30 CFR 250.405 - What are the safety requirements for diesel engines used on a drilling rig?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... engines used on a drilling rig? 250.405 Section 250.405 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations General Requirements § 250.405 What are the safety...

  11. 30 CFR 250.405 - What are the safety requirements for diesel engines used on a drilling rig?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... engines used on a drilling rig? 250.405 Section 250.405 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations General Requirements § 250.405 What are the safety...

  12. 30 CFR 250.405 - What are the safety requirements for diesel engines used on a drilling rig?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... engines used on a drilling rig? 250.405 Section 250.405 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations General Requirements § 250.405 What are the safety...

  13. New drilling rigs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tubb, M.

    1983-03-01

    Petronas recently commissioned its first offshore jack-up drilling rig at Promet, Singapore. The $49 million jack-up Parameswara will undertake both exploration and development activities in Petronas Carigali's exploration block off the eastern coast of Malaysia. The block measures 19,800 sq. km. Initially, the rig will be located at the Duyong gas field. Based on Baker Marine Corporation's BMC 300 design, the 65 X 64 X 8 m rig is capable of working in water depths of up to 91.4 m and is able to drill to a depth of 7,600 m. It has three triangular open-lattice truss-type legs, each 131more » m long. Prominent features include four-tier living quarters which can house 90 men, three cranes of boom length 30.48 m each, a helideck, mess hall, galley, and recreation room. The rig is built to American Bureau of Shipping standards. This paper describes the transport, installation and ballast operations involved in situating the Petronas rig in the Duyong field.« less

  14. Sea Bed Drilling Technology MARUM-MeBo: Overview on recent scientific drilling campaigns and technical developments

    NASA Astrophysics Data System (ADS)

    Freudenthal, Tim; Bergenthal, Markus; Bohrmann, Gerhard; Pape, Thomas; Kopf, Achim; Huhn-Frehers, Katrin; Gohl, Karsten; Wefer, Gerold

    2017-04-01

    The MARUM-MeBo (abbreviation for Meeresboden-Bohrgerät, the German expression for seafloor drill rig) is a robotic drilling system that is developed since 2004 at the MARUM Center for Marine Environmental Sciences at the University of Bremen in close cooperation with Bauer Maschinen GmbH and other industry partners. The MARUM-MeBo drill rigs can be deployed from multipurpose research vessel like, RV MARIA S. MERIAN, RV METEOR, RV SONNE and RV POLARSTERN and are used for getting long cores both in soft sediments as well as hard rocks in the deep sea. The first generation drill rig, the MARUM-MeBo70 is dedicated for a drilling depth of more than 70 m (Freudenthal and Wefer, 2013). Between 2005 and 2016 it was deployed on 17 research expeditions and drilled about 3 km into different types of geology including carbonate and crystalline rocks, gas hydrates, glacial tills, sands and gravel, glacial till and hemipelagic mud with an average recovery rate of about 70 %. We used the development and operational experiences of MARUM-MeBo70 for the development of a second generation drill rig MARUM-MeBo200. This drill rig is dedicated for conducting core drilling down to 200 m below sea floor. After successful sea trials in the North Sea in October 2014 the MeBo200 was used on a scientific expedition on the research vessel RV SONNE (SO247) in March/April 2016. During 12 deployments we drilled altogether 514 m in hemipelagic sediments with volcanic ashes as well as in muddy and sandy slide deposits off New Zealand. The average core recovery was about 54%. The maximum drilling depth was 105 m below sea floor. Developments for the MeBo drilling technology include the development of a pressure core barrel that was successfully deployed on two research expeditions so far. Bore hole logging adds to the coring capacity. Several autonomous logging probes have been developed in the last years for a deployment with MeBo in the logging while tripping mode - a sonic probe measuring in situ p-wave velocity being the latest development. Various bore hole monitoring systems where developed and deployed with the MeBo system. They allow for long-term monitoring of pressure variability within the sealed bore holes. References: Freudenthal, T and Wefer, G (2013) Drilling cores on the sea floor with the remote-controlled sea floor drilling rig MeBo. Geoscientific Instrumentation, Methods and Data Systems, 2(2). 329-337. doi:10.5194/gi-2-329-2013

  15. 30 CFR 250.405 - What are the safety requirements for diesel engines used on a drilling rig?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What are the safety requirements for diesel... Gas Drilling Operations General Requirements § 250.405 What are the safety requirements for diesel engines used on a drilling rig? You must equip each diesel engine with an air take device to shut down the...

  16. Practical calibration of design data to technical capabilities of horizontal directional drilling rig

    NASA Astrophysics Data System (ADS)

    Toropov, S. Yu; Toropov, V. S.

    2018-05-01

    In order to design more accurately trenchless pipeline passages, a technique has been developed for calculating the passage profile, based on specific parameters of the horizontal directional drilling rig, including the range of possible drilling angles and a list of compatible drill pipe sets. The algorithm for calculating the parameters of the trenchless passage profile is shown in the paper. This algorithm is based on taking into account the features of HDD technology, namely, three different stages of production. The authors take into account that the passage profile is formed at the first stage of passage construction, that is, when drilling a pilot well. The algorithm involves calculating the profile by taking into account parameters of the drill pipes used and angles of their deviation relative to each other during the pilot drilling. This approach allows us to unambiguously calibrate the designed profile for the HDD rig capabilities and the auxiliary and navigation equipment used in the construction process.

  17. Advanced jack up rig breaking U.S. construction drought

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kelly, P.

    1997-03-10

    A new heavy duty jack up, due in mid-1998, will be able to simultaneously drill and produce wells in harsher environments and deeper water than current jack ups in the worldwide fleet. Rowan Cos. Inc.`s Gorilla V is the only mobile offshore drilling unit (MODU) currently under construction in the US. Two more enhanced Gorilla design rigs are planned before the year 2000. The enhanced Gorilla class jack up represents the most technologically advanced jack up unit constructed to date. The rigs are structurally designed to meet year-round weather challenges in the harshest geographical environments. Rising demand for drilling rigs,more » coupled with a dwindling fleet, is generating supply shortages around the world, particularly at the high-specification end of the market. Even increasing the historical retirement age from 20 to 25 years, rig attrition continues at a level of about 18 rigs per year. Apart from the jack up market per se, however, Rowan`s strategy in designing and building enhanced Gorillas is to improve existing jack up drilling technology and offer the versatility to operate as a drilling unit, a mobile production unit, or both simultaneously in either open water locations or alongside existing platforms. The paper discusses the market for these heavy jack-ups, the use of one on the Cohasset project in Nova Scotia, the Gorilla V and enhanced Gorillas, geographical range of use, and MOPU economics.« less

  18. A new scientific drilling infrastructure in Sweden

    NASA Astrophysics Data System (ADS)

    Rosberg, J.-E.; Lorenz, H.

    2012-04-01

    A new scientific drilling infrastructure is currently under commissioning at Lund University in southern Sweden and is intended primarily for Swedish scientific drilling projects. However, it will be available to the scientific community and even industry when not occupied. The drill rig, a crawler mounted Atlas Copco CT20, was funded by the Swedish Research Council (VR) after an application by the Swedish scientific drilling community under the lead of Prof. Leif Bjelm, Lund University. As a national resource it is, together with support of the Swedish Deep Drilling Program (SDDP) and the Swedish membership in ICDP, part of VR's commitment to scientific drilling. The Atlas Copco CT20 is a top modern, versatile diamond wireline core-drilling rig which can handle P, H and N sizes. It can operate on very small drill sites (500-800 m2) and, thus, leaves a minimal environmental footprint. The crawler makes the rig ideal for operations in remote locations. A total of only 3-4 truckloads is necessary for mobilization of the basic drilling equipment. Main technical specifications are: Depth capacity coring, based on vertical water filled hole: P-size to around 1050 m, hole size 123 mm and core size 85 mm. H-size to around 1600 m, hole size 96 mm and core size 63 mm. N-size to around 2500 m, hole size 76 mm and core size 48 mm. Weight: Complete rig including crawler, wet - 23500 kg Dimensions in (length, width, height) transport position: 11560 x 2500 x 3750 mm. Available in-hole equipment: Complete core retrieval system for PQ, HQ and NQ-sizes, including PHD, HRQ (V-Wall) and NRQ (V-Wall) drill rods covering the maximum drilling depth for each size (see rig depth capacity above). Both dual and triple tube for HQ and NQ-sizes. Casing advancers (PW, HW, NW and BW). Casing PWT, HWT, NW and BW. Bits and reamers. Additional equipment: Mud cleaning and mixing system. MWD-system (Measurements While Drilling). Cementing equipment. Fishing tools (Bowen Spear). Blow Out Preventer (BOP). Deviation tools. Wireline packers. And more.

  19. Occupational Fatalities Resulting from Falls in the Oil and Gas Extraction Industry, United States, 2005-2014.

    PubMed

    Mason, Krystal L; Retzer, Kyla D; Hill, Ryan; Lincoln, Jennifer M

    2017-04-28

    During 2003-2013, fatality rates for oil and gas extraction workers decreased for all causes of death except those associated with fall events, which increased 2% annually during 2003-2013 (1). To better understand risk factors for these events, CDC examined fatal fall events in the oil and gas extraction industry during 2005-2014 using data from case investigations conducted by the Occupational Safety and Health Administration (OSHA). Sixty-three fatal falls were identified, accounting for 15% of all fatal events. Among fatal falls, 33 (52%) workers fell from a height of >30 feet (9 meters), and 22 (35%) fell from the derrick board, the elevated work platform located in the derrick (structure used to support machinery on a drilling rig). Fall fatalities occurred most frequently when drilling rigs were being assembled or disassembled at the well site (rigging up or rigging down) (14; 22%) or when workers were removing or inserting drill pipe into the wellbore (14; 22%). Measures that target derrickmen and workers engaged in assembling and disassembling drilling rigs (rigging up and down) could reduce falls in this industry. Companies should annually update their fall protection plans and ensure effective fall prevention programs are in place for workers at highest risk for falls, including providing trainings on proper use, fit, and inspection of personal protective equipment.

  20. Diverter bop system and method for a bottom supported offshore drilling rig

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Roche, J. R.; Alexander, G. G.; Carbaugh, W. L.

    1985-06-25

    A system and method for installing a fluid flow controller and telescoping spools beneath an offshore bottom supported drilling rig rotary table is disclosed. Upper and lower telescoping spools are provided for initially connecting a Diverter/BOP convertible fluid flow controller between structural casing in the well and a permanent housing beneath the drilling rig rotary table. Clamp means are provided for clamping the rig vent line to an opening in the housing wall of the fluid flow controller during drilling of the borehole through the structural casing in preparation for setting and cementing the conductor casing. In that mode, themore » system is adapted as a diverter system. After the well is drilled for the conductor casing and the conductor casing is cemented and cut off at its top, a mandrel is fitted at the top of the conductor casing to which the lower end of the lower spool may be connected. The system may be used in this configuration as a diverter system, or after removal of the vent line and connection of a kill line to the housing outlet, the system may be used as a low pressure blowout preventer system.« less

  1. Test drilling in basalts, Lalamilo area, South Kohala District, Hawaii

    USGS Publications Warehouse

    Teasdale, Warren E.

    1980-01-01

    Test drilling has determined that a downhole-percussion airhammer can be used effectively to drill basalts in Hawaii. When used in conjunction with a foam-type drilling fluid, the hammer-bit penetration rate was rapid. Continuous drill cuttings from the materials penetrated were obtained throughout the borehole except from extremely fractured or weathered basalt zones where circulation was lost or limited. Cementing of these zones as soon as encountered reduced problems of stuck tools, washouts, and loss of drill-cuttings. Supplies and logistics on the Hawaiian Islands, always a major concern, require that all anticipated drilling supplies, spare rig and tool parts, drilling muds and additives, foam, and miscellaneous hardware be on hand before starting to drill. If not, the resulting rig downtime is costly in both time and money. (USGS)

  2. Optimizing Geothermal Drilling: Oil and Gas Technology Transfer

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Denninger, Kate; Eustes, Alfred; Visser, Charles

    There is a significant amount of financial risk associated with geothermal drilling. This study of drilling operations seeks opportunities to improve upon current practices and technologies. The scope of this study included analyzing 21 geothermal wells and 21 oil and gas wells. The goal was to determine a 'Perfect Well' using historical data to compare the best oil and gas well to the best geothermal well. Unfortunately, limitations encountered in the study included missing data (bit records, mud information, etc.) and poor data collection practices An online software database was used to format drilling data to IADC coded daily drillingmore » reports and generate figures for analysis. Six major issues have been found in geothermal drilling operations. These problems include lost circulation, rig/ equipment selection, cementing, penetration rate, drilling program, and time management. As a result of these issues, geothermal drilling averaged 56.4 days longer than drilling comparable oil and gas wells in the wells in this study. Roughly $13.9 million was spent on non-productive time in the 21 geothermal wells, compared with only $1.3 million in the oil and gas wells, assuming a cost of $50,000 per day. Comparable events such as drilling the same sized hole, tripping in/out, cementing, and running the same size casing took substantially less time in the oil and gas wells. Geothermal wells were drilled using older and/or less advanced technology to depths less than 10,000 feet, while oil and gas wells reached 12,500 feet faster with purpose built rigs. A new approach is now underway that will optimize drilling programs throughout the drilling industry using Mechanical Specific Energy (MSE) as a tool to realize efficient drilling processes. Potential improvements for current geothermal operations are: the use of electronic records, real time services, and official glossary terms to describe rig operations, and advanced drilling rigs/technology.« less

  3. Sea Spray and Icing in the Emerging Open Water of the Arctic Ocean

    DTIC Science & Technology

    2013-06-17

    can create copious amounts of sea spray. We anticipate that structures placed in shallow water— wind turbines or drilling rigs, for instance— will...anticipate that structures placed in shallow water— wind turbines or drilling rigs, for instance—will, therefore, experience more episodes of freezing...concentrations of wind -generated sea spray and the resulting spray icing on offshore structures, such as wind turbines and exploration, drilling, and production

  4. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chevallier, J.J.; Quetier, F.P.; Marshall, D.W.

    Sedco Forex has developed an integrated computer system to enhance the technical performance of the company at various operational levels and to increase the understanding and knowledge of the drill crews. This paper describes the system and how it is used for recording and processing drilling data at the rig site, for associated technical analyses, and for well design, planning, and drilling performance studies at the operational centers. Some capabilities related to the statistical analysis of the company's operational records are also described, and future development of rig computing systems for drilling applications and management tasks is discussed.

  5. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    As the search for oil and gas continues into deeper and deeper waters, semisubmersibles and dynamically positioned vessels are becoming larger and more sophisticated. Efforts to reduce downtime resulting from foul weather are apparent in some new rigs designed to have improved motion characteristics. New offshore drilling rigs described include l) a buoy-shaped semisubmersible, the Big Bouy 6000 from the Trosvik Group of Norway, 2) a dynamically positioned drillship to be operated by the Dutch firm Neddrill, 3) Parker Drilling Co.'s helicopter-transportable platform rig, rated for 20,000 ft, 4) a dynamically positioned semisubmersible developed by French drilling contractor Forex Neptune,more » and 5) a reinforced-concrete semisubmersible, the Condrill concept, developed by Norwegian contractor A/S Hoyer-Ellefsen.« less

  6. Continuous injection of an inert gas through a drill rig for drilling into potentially hazardous areas

    DOEpatents

    McCormick, S.H.; Pigott, W.R.

    1997-12-30

    A drill rig for drilling in potentially hazardous areas includes a drill having conventional features such as a frame, a gear motor, gear box, and a drive. A hollow rotating shaft projects through the drive and frame. An auger, connected to the shaft is provided with a multiplicity of holes. An inert gas is supplied to the hollow shaft and directed from the rotating shaft to the holes in the auger. The inert gas flows down the hollow shaft, and then down the hollow auger and out through the holes in the bottom of the auger into the potentially hazardous area. 3 figs.

  7. Continuous injection of an inert gas through a drill rig for drilling into potentially hazardous areas

    DOEpatents

    McCormick, Steve H.; Pigott, William R.

    1997-01-01

    A drill rig for drilling in potentially hazardous areas includes a drill having conventional features such as a frame, a gear motor, gear box, and a drive. A hollow rotating shaft projects through the drive and frame. An auger, connected to the shaft is provided with a multiplicity of holes. An inert gas is supplied to the hollow shaft and directed from the rotating shaft to the holes in the auger. The inert gas flows down the hollow shaft, and then down the hollow auger and out through the holes in the bottom of the auger into the potentially hazardous area.

  8. Optimizing Geothermal Drilling: Oil and Gas Technology Transfer

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tilley, Mitch; Eustes, Alfred; Visser, Charles

    There is a significant amount of financial risk associated with geothermal drilling; however, there are opportunities to improve upon current practices and technologies used. The scope of this drilling operational study included 21 geothermal wells and 21 oil and gas wells. The goal was to determine a 'perfect well' using historical data to compare the best oil and gas well to the best geothermal well. Unfortunately, limitations encountered in the study included missing data (bit records, mud information, etc.), poor data collection, and difficult to ascertain handwriting. An online software database was used to format drilling data to IADC codedmore » daily drilling reports and generate analysis figures. Six major issues have been found in geothermal drilling operations. These problems include lost circulation, rig/equipment selection, cementing, penetration rate, drilling program, and time management. As a result of these issues, geothermal drilling averages 56.4 days longer than drilling comparable oil and gas wells in the wells in this study. Roughly $13.9 million would be lost due to non-productive time in the 21 geothermal wells and only $1.3 million in the oil and gas wells, assuming a cost of $50,000 per day. Comparable events such as drilling the same sized hole, tripping in/out, cementing, and running the same size casing took substantially less time in the oil and gas wells. Geothermal wells were drilled using older and/or less advanced technology to depths less than 10,000 feet, while oil and gas wells reached 12,500 feet faster with purpose built rigs. A new approach is now underway that will optimize drilling programs throughout the drilling industry. It is the use of Mechanical Specific Energy (MSE) as a tool to realize efficient drilling processes. However, a work-flow must also be established in order for there to be an efficient drilling program. Potential improvements for current geothermal operations are: the use of electronic records, real time services, and official glossary terms to describe rig operations, and advanced drilling rigs/technology.« less

  9. Rowan Gorilla I rigged up, heads for eastern Canada

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1984-03-01

    Designed to operate in very hostile offshore environments, the first of the Rowan Gorilla class of self-elevating drilling rigs has been towed to its drilling assignment offshore Nova Scotia. About 40% larger than other jackups, these rigs can operate in 300 ft of water, drilling holes as deep as 30,000 ft. They also feature unique high-pressure and solids control systems that are expected to improve drilling procedures and efficiencies. A quantitative formation pressure evaluation program for the Hewlett-Packard HP-41 handheld calculator computes formation pressures by three independent methods - the corrected d exponent, Bourgoyne and Young, and normalized penetration ratemore » techniques for abnormal pressure detection and computation. Based on empirically derived drilling rate equations, each of the methods can be calculated separately, without being dependent on or influenced by the results or stored data from the other two subprograms. The quantitative interpretation procedure involves establishing a normal drilling rate trend and calculating the pore pressure from the magnitude of the drilling rate trend or plotting parameter increases above the trend line. Mobil's quick, accurate program could aid drilling operators in selecting the casing point, minimizing differential sticking, maintaining the proper mud weights to avoid kicks and lost circulation, and maximizing penetration rates.« less

  10. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Knott, D.

    Floating production system (FPS) technology has come of age in the North Sea. That's apparent in plans to use FPSs to tap two of Northwest Europe's largest offshore oil discoveries in the last 10 years. First North Sea oil production with a floater involved a converted semisubmersible drilling rig. Floaters have been in use for small field development projects ever since. Now, industry's rising interest in FPSs reflects two trends: As the North Sea matures, discoveries are likely to be in deeper, more remote locations; and Operators increasingly are under pressure to slash costs. The paper discusses UK trends, Norway'smore » needs, the Norne field, Norne contract, discovery of oil west of the Shetland Islands, Shell-Esso plans, the UK Machar field test, the UK Fife field, and prospects for other potential floater developments.« less

  11. Managing Geothermal Exploratory Drilling Risks Drilling Geothermal Exploration and Delineation Wells with Small-Footprint Highly Portable Diamond Core Drills

    NASA Astrophysics Data System (ADS)

    Tuttle, J.; Listi, R.; Combs, J.; Welch, V.; Reilly, S.

    2012-12-01

    Small hydraulic core rigs are highly portable (truck or scow-mounted), and have recently been used for geothermal exploration in areas such as Nevada, California, the Caribbean Islands, Central and South America and elsewhere. Drilling with slim diameter core rod below 7,000' is common, with continuous core recovery providing native-state geological information to aid in identifying the resource characteristics and boundaries; this is a highly cost-effective process. Benefits associated with this innovative exploration and delineation technology includes the following: Low initial Capital Equipment Cost and consumables costs Small Footprint, reducing location and road construction, and cleanup costs Supporting drill rod (10'/3meter) and tools are relatively low weight and easily shipped Speed of Mobilization and rig up Reduced requirements for support equipment (cranes, backhoes, personnel, etc) Small mud systems and cementing requirements Continuous, simplified coring capability Depth ratings comparable to that of large rotary rigs (up to ~10,000'+) Remote/small-location accessible (flown into remote areas or shipped in overseas containers) Can be scow or truck-mounted This technical presentation's primary goal is to share the technology of utilizing small, highly portable hydraulic coring rigs to provide exploratory drilling (and in some cases, production drilling) for geothermal projects. Significant cost and operational benefits are possible for the Geothermal Operator, especially for those who are pursuing projects in remote locations or countries, or in areas that are either inaccessible or in which a small footprint is required. John D. Tuttle Sinclair Well Products jtuttle@sinclairwp.com

  12. Tough new jack-up for rough seas

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1984-01-01

    Rowan Company's new deepwater jack-up, Rowan Gorilla I, is scheduled to spud its initial well off the east coast of Canada later this month for a consortium of oil companies including Bow Valley-Husky and ATS Exploration Ltd. The new rig's class designation--Gorilla--reflects designers' expectations for the drilling unit that is suited to work in virtually all ice-free hostile environmental areas of the world. Rowan's confidence in the design, built by Marathon LeTourneau's Vicksburg, Mississippi yard, is reiterated by the fact that two additional rigs in the Gorilla class are being built. Rowan Gorilla II is being constructed at Marathon's Singaporemore » yard, and the Rowan Gorilla III is in early construction stages at the firm's Vicksburg yard. The three Gorilla-class rigs will cost in excess of $85 million each, including owner-furnished drilling equipment. This, according to owners, will make them among the costliest jack-ups in the world. Another record being claimed by the Gorilla-class drilling units is that they are the largest jack-ups in the world. Fully outfitted, a Gorilla contains 16,000 tons of steel. Its triangular hull measures 297 ft from bow to stern and 292 ft across the stern. The rig has a variable load of 2,750 tons for drilling consumables and 42,265 sq ft of deck space. Its ample storage capacity, along with its 503-ft leg length and certain design features, makes the rig compatible with hostile offshore areas, where it can continue drilling for long periods unattended.« less

  13. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chevallier, J.; Turner, L.

    There's a great deal of data recorded during drilling operations on rigs these days, but it is seldom well utilized. The operator's company person relies upon mud loggers for collecting and recording most information. The methods used to process and display this information are often inadequate for those who need it the most the driller and toolpusher. Drilling contractor personnel usually have only rudimentary displays of drilling parameters, and practically no serious method of analysis except for daily paper reports. These are cumbersome to use and provide only incomplete data, after the fact. The MDS system, presented in this article,more » is a new information and alarm network, which rectifies this situation by bringing to the rig, for the first time, the latest in sensor and computer technologies. This system acquires key drilling data on the rig floor, pump room, and return line, and displays it in a clear graphical format to both the driller and the toolpusher in real time. It also provides the toolpusher with a workstation for easy access to the same information for evaluation and planning of the drilling program.« less

  14. Drilling Rig Operation Mode Recognition by an Artificial Neuronet

    NASA Astrophysics Data System (ADS)

    Abu-Abed, Fares; Borisov, Nikolay

    2017-11-01

    The article proposes a way to develop a drilling rig operation mode classifier specialized to recognize pre-emergency situations appearable in commercial oil-and-gas well drilling. The classifier is based on the theory of image recognition and artificial neuronet taught on real geological and technological information obtained while drilling. To teach the neuronet, a modified backpropagation algorithm that can teach to reach the global extremum of a target function has been proposed. The target function was a relative recognition error to minimize in the teaching. Two approaches to form the drilling rig pre-emergency situation classifier based on a taught neuronet have been considered. The first one involves forming an output classifier of N different signals, each of which corresponds to a single recognizable situation and, and can be formed on the basis of the analysis of M indications, that is using a uniform indication vocabulary for all recognized situations. The second way implements a universal classifier comprising N specialized ones, each of which can recognize a single pre-emergency situation and having a single output.

  15. 30 CFR 250.405 - What are the safety requirements for diesel engines used on a drilling rig?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 2 2011-07-01 2011-07-01 false What are the safety requirements for diesel... are the safety requirements for diesel engines used on a drilling rig? You must equip each diesel engine with an air take device to shut down the diesel engine in the event of a runaway. (a) For a diesel...

  16. 76 FR 2254 - Notice of Arrival on the Outer Continental Shelf

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-13

    ... of arrival for floating facilities, mobile offshore drilling units (MODUs), and vessels planning to... Ship Security Certificate. MMS Minerals Management Service. MODU Mobile Offshore Drilling Unit. NAICS... rule outlines the procedures that owners or operators of floating facilities, mobile offshore drilling...

  17. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dudleson, B.; Arnold, M.; McCann, D.

    Rapid detection of unexpected drilling events requires continuous monitoring of drilling parameters. A major R and D program by a drilling contractor has led to the introduction of a computerized monitoring system on its offshore rigs. System includes advanced color graphics displays and new smart alarms to help both contractor and operator personnel detect and observe drilling events before they would normally be apparent with conventional rig instrumentation. This article describes a module of this monitoring system, which uses expert system technology to detect the earliest stages of drillstring washouts. Field results demonstrate the effectiveness of the smart alarm incorporatedmore » in the system. Early detection allows the driller to react before a twist-off results in expensive fishing operations.« less

  18. Development and testing of a Mudjet-augmented PDC bit.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Black, Alan; Chahine, Georges; Raymond, David Wayne

    2006-01-01

    This report describes a project to develop technology to integrate passively pulsating, cavitating nozzles within Polycrystalline Diamond Compact (PDC) bits for use with conventional rig pressures to improve the rock-cutting process in geothermal formations. The hydraulic horsepower on a conventional drill rig is significantly greater than that delivered to the rock through bit rotation. This project seeks to leverage this hydraulic resource to extend PDC bits to geothermal drilling.

  19. Final report on the design and development of a Rolling Float Meter for drilling-fluid outflow measurement

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Staller, G.E.; Westmoreland, J.J.; Whitlow, G.L.

    1998-03-01

    Lost circulation, which is the loss of well drilling fluids to the formation while drilling, is a common problem encountered while drilling geothermal wells. The rapid detection of the loss of well drilling fluids is critical to the successful and cost-effective treatment of the wellbore to stop or minimize lost circulation. Sandia National Laboratories has developed an instrument to accurately measure the outflow rate of drilling fluids while drilling. This instrument, the Rolling Float Meter, has been under development at Sandia since 1991 and is now available for utilization by interested industry users. This report documents recent Rolling Float Metermore » design upgrades resulting from field testing and industry input, the effects of ongoing testing and evaluation both in the laboratory and in the field, and the final design package that is available to transfer this technology to industry users.« less

  20. 30 CFR 251.7 - Test drilling activities under a permit.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Test drilling activities under a permit. 251.7... GEOLOGICAL AND GEOPHYSICAL (G&G) EXPLORATIONS OF THE OUTER CONTINENTAL SHELF § 251.7 Test drilling activities... of drilling activities; (ii) A description of your drilling rig, indicating the important features...

  1. Gorilla jackup offers drilling advantages

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tanner, R.

    1983-12-01

    The Rowan Gorilla I, recently completed for Rowan Cos. Inc. at Vicksburg, Mississippi, is a 32-million-lb jackup drilling rig - the largest ever built. The rig is designed for use in hostile environments, where it can endure for long periods without resupply. The Gorilla's hull is 297 ft. long, 292 ft. wide and 30 ft. deep. It is designed to survive 90 ft. waves and 82-knots winds in up to 328 ft. of ice-free water.

  2. 30 CFR 250.418 - What additional information must I submit with my APD?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Applying for A Permit to Drill § 250.418 What additional... the drilling rig and major drilling equipment, if not already on file with the appropriate District...

  3. Don't mess with H/sub 2/S

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1987-09-01

    The American Petroleum Institute issued a report on ''Recommended Practices for Safe Drilling of Wells Containing Hydrogen Sulfide.'' The study (RP49) updates a first edition published in September 1974. It provides a solid overview of preventive steps that should be taken to safeguard crew and equipment when drilling through H/sub 2/S zones. Discussions cover personnel training, protective equipment, wellsite layout, rig and well equipment, general rig operations and contingency planning and emergency procedures. This article summarizes the report.

  4. 30 CFR 250.1604 - General requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... moving equipment such as a well-drilling, well-completion, or well-workover rig or associated equipment..., platform age, and previous stresses. (f) Traveling-block safety device. All drilling units being used for...

  5. 30 CFR 250.1604 - General requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... moving equipment such as a well-drilling, well-completion, or well-workover rig or associated equipment..., platform age, and previous stresses. (f) Traveling-block safety device. All drilling units being used for...

  6. 30 CFR 250.1604 - General requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... moving equipment such as a well-drilling, well-completion, or well-workover rig or associated equipment..., platform age, and previous stresses. (f) Traveling-block safety device. All drilling units being used for...

  7. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    This article describes a unique rig designed by SEDCO FOREX operating in the Paris basin of France. Built to drill clusters of wells from a single pad, Rig 47 significantly reduces the time needed to move from well to well on a pad and from location to location.

  8. Preliminary Research on Possibilities of Drilling Process Robotization

    NASA Astrophysics Data System (ADS)

    Pawel, Stefaniak; Jacek, Wodecki; Jakubiak, Janusz; Zimroz, Radoslaw

    2017-12-01

    Nowadays, drilling & blasting is crucial technique for deposit excavation using in hard rock mining. Unfortunately, such approach requires qualified staff to perform, and consequently there is a serious risk related to rock mechanics when using explosives. Negative influence of explosives usage on safety issues of underground mine is a main cause of mining demands related to elimination of people from production area. Other aspects worth taking into consideration are drilling precision according to drilling pattern, blasting effectiveness, improvement of drilling tool reliability etc. In the literature different drilling support solutions are well-known in terms of positioning support systems, anti-jamming systems or cavity detection systems. For many years, teleoperation of drilling process is also developed. Unfortunately, available technologies have so far not fully met the industries expectation in hard rock. Mine of the future is expected to incorporate robotic system instead of current approaches. In this paper we present preliminary research related to robotization of drilling process and possibilities of its application in underground mine condition. A test rig has been proposed. To simulate drilling process several key assumptions have been accepted. As a result, algorithms for automation of drilling process have been proposed and tested on the test rig. Experiences gathered so far underline that there is a need for further developing robotic system for drilling process.

  9. DOE Office of Scientific and Technical Information (OSTI.GOV)

    McCann, D.; White, D.

    This paper reports on a smart alarm system installed on a number of offshore rigs and one land rig which can detect kicks more quickly than conventional systems. This rapid kick detection improves rig safety because the smaller the detected influx, the easier it is to control the well. The extensive computerized monitoring system helps drilling personnel detect fluid influxes and fluid losses before the changes in flow would normally be apparent.

  10. 30 CFR 250.1616 - Supervision, surveillance, and training.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Sulphur... supervision of drilling operations at all times. (b) From the time drilling operations are initiated and until the well is completed or abandoned, a member of the drilling crew or the toolpusher shall maintain rig...

  11. Oil rigs and offshore sport fishing in Louisiana

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dugas, R.; Guillory, V.; Fischer, M.

    Forty years ago, offshore sport fishing in Louisiana was almost nonexistent. Offshore oil drilling platforms are the primary cause of the present increase in sport fishing in this area. Algae and other organisms forming the first step in the food chain cluster around the subsurface structures of the rigs, attracting fish that seek food and shelter. Major game species frequenting these rigs are identified. (3 photos, 22 references, 2 tables)

  12. Establish a Data Transmission Platform of the Rig Based on the Distributed Network

    NASA Astrophysics Data System (ADS)

    Bao, Zefu; Li, Tao

    In order to control in real-time ,closed-loop feedback the information, saving the money and labor,we distribute a platform of network data. It through the establishment of the platform in the oil drilling to achieve the easiest route of each device of the rig that conveying timely. The design proposed the platform to transfer networking data by PA which allows the rig control for optimal use. Against the idea,achieving first through on-site cabling and the establishment of data transmission module in the rig monitoring system. The results of standard field application show that the platform solve the problem of rig control.

  13. An inexpensive and portable drill rig for bedrock groundwater studies in headwater catchments

    Treesearch

    C. Gabrielli; J.J. McDonnell

    2011-01-01

    Bedrock groundwater dynamics in headwater catchments are poorly understood and poorly characterized. Here, we present an inexpensive and portable bedrock drilling system designed for use in remote locations. Our system is capable of drilling bedrock wells up to 11 m deep and 38 mm in diameter in a wide range of bedrock types. The drill consists of a lawn mower engine...

  14. 78 FR 48180 - Consolidation of Officer in Charge, Marine Inspection For Outer Continental Shelf Activities...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-07

    ... for all Mobile Offshore Drilling Units and Floating Outer Continental Shelf Facilities (as defined in... Commander. Vessels requiring Coast Guard inspection include Mobile Offshore Drilling Units (MODUs), Floating... engage directly in oil and gas exploration or production in the offshore waters of the Eighth Coast Guard...

  15. 30 CFR 250.418 - What additional information must I submit with my APD?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Applying for A Permit to Drill § 250.418 What additional information must I submit with my APD? You must include the following with the APD: (a) Rated capacities of the drilling rig...

  16. 30 CFR 250.418 - What additional information must I submit with my APD?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Applying for A Permit to Drill § 250.418 What additional information must I submit with my APD? You must include the following with the APD: (a) Rated capacities of the drilling rig...

  17. 30 CFR 250.418 - What additional information must I submit with my APD?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Applying for A Permit to Drill § 250.418 What additional information must I submit with my APD? You must include the following with the APD: (a) Rated capacities of the drilling rig...

  18. 30 CFR 250.418 - What additional information must I submit with my APD?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Applying for A Permit to Drill § 250.418 What additional information must I submit with my APD? You must include the following with the APD: (a) Rated capacities of the drilling rig and major...

  19. Methodology for reducing energy and resource costs in construction of trenchless crossover of pipelines

    NASA Astrophysics Data System (ADS)

    Toropov, V. S.

    2018-05-01

    The paper suggests a set of measures to select the equipment and its components in order to reduce energy costs in the process of pulling the pipeline into the well in the constructing the trenchless pipeline crossings of various materials using horizontal directional drilling technology. A methodology for reducing energy costs has been developed by regulating the operation modes of equipment during the process of pulling the working pipeline into a drilled and pre-expanded well. Since the power of the drilling rig is the most important criterion in the selection of equipment for the construction of a trenchless crossover, an algorithm is proposed for calculating the required capacity of the rig when operating in different modes in the process of pulling the pipeline into the well.

  20. 75 FR 75662 - Sunshine Act Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-06

    ..., union representatives and industry groups' to discuss regulation of offshore drilling operations in the... from leading safety experts involved in offshore drilling activities from countries including the... be holding an all day symposium entitled ``International Models of Offshore Oil Rig Regulation'' on...

  1. Application program of CRUST-1 10km continental scientific drilling rig in SK-2 scientific drilling well

    NASA Astrophysics Data System (ADS)

    Sun, Youhong; Gao, Ke; Yu, Ping; Liu, Baochang; Guo, Wei; Ma, Yinlong; Yang, Yang

    2014-05-01

    SK-2 Well is located in DaQing city,where is site of the largest oil field in China,Heilongjiang province, north-east of China.The objective of SK-2 well is to obtain full cores of cretaceous formation in Song Liao basin,and to build the time tunnel of Cretaceous greenhouse climate change,and to clarify the causes,processes and results of the formations of DaQing oil field. This will ensure to achieve our ultimate goals,to test the CRUST-1 drilling rig and improve China's deep scientific drilling technology,to form the scientific drilling technology,method and system with independent intellectual property rights,and to provide technical knowledge and information for China's ten kilometers super-deep scientific drilling technical resources.SK-2 Well is at 6400 meter depth, where the drilling inclination is 90 degree and the continuous coring length is 3535 meter that from 2865 to 6400 meter,the recovery rate of the core is greater or equal to 95 percent with 100 millimeters core diameter and 3.9 degree per 100 meter geothermal gradient.The CRUST-1 rig is designated with special drilling equipment for continental scientific drilling combined to the oil drilling equipment ability with advanced geological drilling technology which is highly automatic and intelligent. CRUST-1 drilling ability is 10000 meter with the maximum hook load 700 tons, the total power is 4610 Kilowatt.CRUST-1 will be integrated with a complete set of automation equipment,including big torque hydraulic top drive,high accuracy automatic drilling rod feeding system, suspended automatic drill string discharge device,hydraulic intelligent iron roughneck,and hydraulic automatic catwalk to fully meet the drilling process requirements of SK-2.Designed with advanced drilling technique for 260 degree in the bottom of SK-2 well and hard rock,including the drilling tools of high temperature hydraulic hammer,high temperature resistance and high strength aluminum drill pipe,high temperature preparation of mud treatment and high temperature resistant cementing materials, and bionic bits,that is coupling bionic PDC tooth bit and diamond-impregnated bit for hard rock.All parts of CRUST-1 were successfully assembled along with the derrick and base lift and transported about 3456 kilometers from manufacture,GuangHan city in southwest China's Sichuan province,to the well site of SK-2 in end of 2013.SK-2 will be finished during next 4 years.

  2. Exposure to oil mist and oil vapour during offshore drilling in norway, 1979-2004.

    PubMed

    Steinsvåg, Kjersti; Bråtveit, Magne; Moen, Bente E

    2006-03-01

    To describe personal exposure to airborne hydrocarbon contaminants (oil mist and oil vapour) from 1979 to 2004 in the mud-handling areas of offshore drilling facilities operating on the Norwegian continental shelf when drilling with oil-based muds. Qualitative and quantitative information was gathered during visits to companies involved in offshore oil and gas production in Norway. Monitoring reports on oil mist and oil vapour exposure covered 37 drilling facilities. Exposure data were analysed using descriptive statistics and by constructing linear mixed-effects models. Samples had been taken during the use of three generations of hydrocarbon base oils, namely diesel oils (1979-1984), low-aromatic mineral oils (1985-1997) and non-aromatic mineral oils (1998-2004). Sampling done before 1984 showed high exposure to diesel vapour (arithmetic mean, AM = 1217 mg m(-3)). When low-aromatic mineral oils were used, the exposure to oil mist and oil vapour was 4.3 and 36 mg m(-3), and the respective AMs for non-aromatic mineral oils were reduced to 0.54 and 16 mg m(-3). Downward time trends were indicated for both oil mist (6% per year) and oil vapour (8% per year) when the year of monitoring was introduced as a fixed effect in a linear mixed-effects model analysis. Rig type, technical control measures and mud temperature significantly determined exposure to oil mist. Rig type, type of base oil, viscosity of the base oil, work area, mud temperature and season significantly determined exposure to oil vapour. Major decreases in variability were found for the between-rig components. Exposure to oil mist and oil vapour declined over time in the mud-handling areas of offshore drilling facilities. Exposure levels were associated with rig type, mud temperature, technical control measures, base oil, viscosity of the base oil, work area and season.

  3. Occupational exposure to airborne contaminants during offshore oil drilling.

    PubMed

    Kirkhus, Niels E; Thomassen, Yngvar; Ulvestad, Bente; Woldbæk, Torill; Ellingsen, Dag G

    2015-07-01

    The aim was to study exposure to airborne contaminants in oil drillers during ordinary work. Personal samples were collected among 65 drill floor workers on four stationary and six moveable rigs in the Norwegian offshore sector. Air concentrations of drilling mud were determined based on measurements of the non-volatile mud components Ca and Fe. The median air concentration of mud was 140 μg m(-3). Median air concentrations of oil mist (180 μg m(-3)), oil vapour (14 mg m(-3)) and organic carbon (46 μg m(-3)) were also measured. All contaminants were detected in all work areas (drill floor, shaker area, mud pits, pump room, other areas). The highest air concentrations were measured in the shaker area, but the differences in air concentrations between working areas were moderate. Oil mist and oil vapour concentrations were statistically higher on moveable rigs than on stationary rigs, but after adjusting for differences in mud temperature the differences between rig types were no longer of statistical significance. Statistically significant positive associations were found between mud temperature and the concentrations of oil mist (Spearman's R = 0.46) and oil vapour (0.39), and between viscosity of base oil and oil mist concentrations. Use of pressure washers was associated with higher air concentrations of mud. A series of 18 parallel stationary samples showed a high and statistically significant association between concentrations of organic carbon and oil mist (r = 0.98). This study shows that workers are exposed to airborne non-volatilized mud components. Air concentrations of volatile mud components like oil mist and oil vapour were low, but were present in all the studied working areas.

  4. Jackup placement calls for extreme care

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sharples, B.P.M.; Gorman, M.T.; Firstenberg, C.E.

    1986-05-01

    Jackup drilling rigs are exceedingly useful tools for exploration. The integrity of the jackup rig is very dependent on the specific location at which the unit is operating. Therefore, an accurate assessment of the environment for the location is essential. For safe operations, independent assessments should be made to establish the safety of the rig for specific sites and environments. While it is the task of the designer and the builder to produce a jackup which is suitable for the rigors of operation, it is the responsibility of the owner to operate the unit within an acceptable level of risk.more » When owners rely upon marine surveyors, with the aid of their organizations' criteria, to decide the safety of a jackup on a location, they should be acutely aware of the criteria and assumptions in which they are placing their faith. When a drilling contractor instructs his marine surveyor to assess the suitability of a rig for a location by performing a site-specific analysis, the assumptions made in the calculations can make the results vary widely.« less

  5. Diverter/bop system and method for a bottom supported offshore drilling rig

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Roche, J.R.; Alexander, G.G.; Carbaugh, W.L.

    1986-07-01

    A system is described adapted for alternative use as a diverter or a blowout preventer for a bottom supported drilling rig and adapted for connection to a permanent housing attached to rig structural members beneath a drilling rig rotary table, the permanent housing having an outlet connectable to a rig fluid system flow line. The system consists of: a fluid flow controller having a controller housing with a lower cylindrical opening and an upper cylindrical opening and a vertical path therebetween and a first outlet passage and a second outlet passage provided in its wall, a packing element disposed withinmore » the controller housing, and annular piston means adapted for moving from a first position to a second position, whereby in the first position the piston means wall prevents interior fluid from communicating with the outlet passages in the controller housing wall and in the second position the piston means wall allows fluid communication of interior fluid with the outlet passages and urges the annular packing element to close about an object extending through the bore of the controller housing or to close the vertical flow path through through the controller housing in the absence of any object in the vertical flow path, means for connecting a vent line to the outlet passage provided in the controller housing wall, a lower telescoping spool having a lower joining means at its lower end for joining alternatively to structural casing or to a mandrel connected to a conductor string cemented within the structural casing and an upper connection means at its upper end for connection to the lower cylindrical opening of the fluid flow controller, and an upper telescoping spool having a lower connection means for connection to the upper cylindrical opening of the fluid flow controller.« less

  6. Major mishaps among mobile offshore drilling units, 1955-1981: time trends and fatalities.

    PubMed

    Clemmer, D I; Diem, J E

    1985-03-01

    Major mishaps among mobile offshore drilling units worldwide from 1955-1981 were identified from industry and government sources. Based on annual numbers of rigs in service and typical staffing patterns, annual mishap rates and fatality rates for rig types and mishap categories were computed. While the frequency of major mishaps has increased in recent years, the mishap rate per 100 rig-years of service has remained stable. The overall stability obscures the fact that jack-up rigs have had an increasing mishap rate while the rate for other rig types combined has gradually declined. Although the fatal mishap rate has also remained constant, the annual fatality rate per 100 000 full time equivalent (FTE) workers has risen sharply. This can be attributed to increasing numbers of lives lost in environmental mishaps while deaths from operational mishaps have declined. There were 344 fatalities during the 27-year period. Although an average of some 13 deaths per year worldwide appears minimal, the relatively small size of the workforce gives this number significance particularly when it is noted that 'occupational' fatalities, those occurring in the course of routine operations, are not included. The overall fatality rate secondary to major mishaps was 84.3 per 100 000 FTE worker-years.

  7. Blowout Monitor

    NASA Technical Reports Server (NTRS)

    1994-01-01

    C Language Integrated Production System (CLIPS), a NASA-developed software shell for developing expert systems, has been embedded in a PC-based expert system for training oil rig personnel in monitoring oil drilling. Oil drilling rigs if not properly maintained for possible blowouts pose hazards to human life, property and the environment may be destroyed. CLIPS is designed to permit the delivery of artificial intelligence on computer. A collection of rules is set up and, as facts become known, these rules are applied. In the Well Site Advisor, CLIPS provides the capability to accurately process, predict and interpret well data in a real time mode. CLIPS was provided to INTEQ by COSMIC.

  8. 30 CFR 250.283 - When must I revise or supplement the approved EP, DPP, or DOCD?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) Change the type of production or significantly increase the volume of production or storage capacity; (4... when you propose to: (1) Change the type of drilling rig (e.g., jack-up, platform rig, barge..., tension leg platform), or transportation mode (e.g., pipeline, barge); (2) Change the surface location of...

  9. NOx Emissions from Oil and Gas Production in the North Sea

    NASA Astrophysics Data System (ADS)

    Lee, J. D.; Foulds, A.; Purvis, R.; Vaughan, A. R.; Carslaw, D.; Lewis, A. C.

    2015-12-01

    North Sea oil is a mixture of hydrocarbons, comprising liquid petroleum and natural gas, produced from petroleum reservoirs beneath the North Sea. As of January 2015, the North Sea is the world's most active offshore drilling region with 173 rigs drilling. During the summer of 2015, a series of survey flights took place on the UKs FAAM BAe 146 research aircraft with the primary aim to assess background methane (and other hydrocarbons) levels in the drilling areas of the North Sea. Also measured were Nitrogen Oxides (NO and NO2), which are emitted from almost all combustion processes and are a key air pollutant, both directly and as a precursor to ozone (O3). The oil and gas platforms in the North Sea are often manned and require significant power generation and support vessels for their continued operation, processes that potentially emit significant amounts of NOx into an otherwise relative clean environment. During these flights we were able to measure the NO­­­x (and any subsequently produced O3) emitted from specific rigs, as well as the NOx levels in the wider North Sea oil and gas production region (see figure for example). NOx mixing ratios of <10 ppbv were frequently observed in plumes, with significant perturbation to the wider North Sea background levels. NOx emissions from the rigs are point sources within the UKs National Atmospheric Emission Inventory (NAEI) and the measurements taken in plumes from individual rigs are used to assess the accuracy of these estimates.

  10. MTR CAISSONS WERE DRILLED INTO BEDROCK. IN CENTER OF VIEW, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    MTR CAISSONS WERE DRILLED INTO BEDROCK. IN CENTER OF VIEW, CONCRETE FLOWS FROM TRUCK INTO DRUM, WHICH IS LOWERED INTO CAISSON AND RELEASED AT BOTTOM OF HOLE. BEYOND, TRUCK-MOUNTED DRILLING RIG DRILLS HOLE FOR ANOTHER CAISSON NEAR EDGE OF EXCAVATION. MATERIAL REMOVED FROM HOLE IS CARRIED BY CONVEYOR TO WAITING TRUCK. INL NEGATIVE NO. 307. Unknown Photographer, 6/1950. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  11. The Aerodynamic Drag of Five Models of Side Floats N.A.C.A. Models 51-E, 51-F, 51-G, 51-H, 51-J

    NASA Technical Reports Server (NTRS)

    House, R O

    1938-01-01

    The drag of five models of side floats was measured in the N.A.C.A. 7- by 10-foot wind tunnel. The most promising method of reducing the drag of floats indicated by these tests is lowering the angle at which the floats are rigged. The addition of a step to a float does not always increase the drag in the flying range, floats with steps sometimes having lower drag than similar floats without steps. Making the bow chine no higher than necessary might result in a reduction in air drag because of the lower angle of pitch of the chines. Since side floats are used formally to obtain lateral stability when the seaplane is operating on the water at slow speeds or at rest, greater consideration can be given to factors affecting aerodynamic drag than is possible for other types of floats and hulls.

  12. International Oil and Gas Exploration and Development

    EIA Publications

    1993-01-01

    Presents country level data on oil reserves, oil production, active drilling rigs, seismic crews, wells drilled, oil reserve additions, and oil reserve to production ratios (R/P ratios) for about 85 countries, where available, from 1970 through 1991. World and regional summaries are given in both tabular and graphical form.

  13. Special mobile rescue unit can speed recovery

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1977-04-01

    Since time is often a matter of life and death in a mine disaster, Mine Emergency Operations (MEO) personnel are prepared to begin rescue work at any mining site in the eastern part of the United States within six hours of notification, and within no more than nine hours in the western United States. The entire MEO force, including vans, trucks, bulldozers to clear and level the drilling site, seismic equipment, and the big drilling rig can be on any site within less than 20 hours of a disaster. The speed of deployment is made possible in some measure bymore » a special agreement between MESA and the United States Air Force, which stands ready 24 hours a day to dispatch giant C-130 cargo aircraft to airlift the tons of bulky MEO equipment. While the big drilling rig is usually taken to disaster sites by highway, it can also be airlifted when necessary.« less

  14. Cleaning up: Colleges Do Their Part to Revitalize the Gulf

    ERIC Educational Resources Information Center

    Violino, Bob

    2011-01-01

    When the deepwater horizon offshore oil drilling rig exploded in the Gulf of Mexico in April 2010, 11 crewmen were killed; the accident led to the largest oil spill in U.S. history. Forty workers on the rig managed to escape the catastrophe, thanks largely to a safety-training program they had taken at community colleges in the Louisiana Community…

  15. Cholera on a Gulf Coast oil rig.

    PubMed

    Johnston, J M; Martin, D L; Perdue, J; McFarland, L M; Caraway, C T; Lippy, E C; Blake, P A

    1983-09-01

    A single case of severe diarrhea on a floating Texas oil rig was followed two days later by what proved to be the largest outbreak of cholera in the United States in over a century. After isolation of toxigenic Vibrio cholerae El Tor Inaba of the typical United States phage type from the index patient's stool, the ensuing investigation detected 14 additional cases of cholera and one asymptomatic infection serologically. Infection was associated with eating rice on the oil rig on a particular day (P = 0.03) when an open valve permitted the rig's drinking-water system to be contaminated by canal water containing sewage (including that from the index patient) discharged from the rig. The rice had been rinsed in the contaminated water after cooking, and before being served it had been maintained at a temperature that allows V. cholerae 01 to multiply. Toxigenic V. cholerae 01 is persisting in the United States, and large common-source outbreaks of cholera can occur if proper sanitation is not maintained.

  16. Contractors on road to recovery

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stremel, K.

    1984-06-01

    Ravaged by two years of spotty markets and minimal profits, the well service industry is on the road to recovery. A discussion of the increase and decrease of drilling rigs and drilling activity in the Rocky Mountain region is given. It also discusses the current trend of well service costs and the direction it should take in the future.

  17. Surface drilling technologies for Mars

    NASA Technical Reports Server (NTRS)

    Blacic, J. D.; Rowley, J. C.; Cort, G. E.

    1986-01-01

    Rock drilling and coring conceptual designs for the surface activities associated with a manned Mars mission are proposed. Straightforward extensions of equipment and procedures used on Earth are envisioned for the sample coring and shallow high explosive shot holes needed for tunneling and seismic surveying. A novel rocket exhaust jet piercing method is proposed for very rapid drilling of shot holes required for explosive excavation of emergency radiation shelters. Summaries of estimated equipment masses and power requirements are provided, and the indicated rotary coring rigs are scaled from terrestrial equipment and use compressed CO2 from the Martian atmosphere for core bit cooling and cuttings removal. A mass of 120 kg and power of 3 kW(e) are estimated for a 10 m depth capability. A 100 m depth capacity core rig requires about 1150 kg and 32 km(e). The rocket exhaust jet equipment devised for shallow (3m) explosive emplacement shot holes requires no surface power beyond an electrical ignition system, and might have a 15 kg mass.

  18. Libya: a dynamic Arab oil power

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hobbs, M.

    1965-06-01

    Libya has become the most dynamic oil-producing nation in the world. Less than 4 yr ago, Libya exported only peanuts, olive oil, and hides. Today, in crude-oil production Libya ranks eighth in the world, with daily production of 1.2 million bbl. Twenty-five oil companies hold concessions and have spent $1.3 billion developing reserves. Petroleum has brought new wealth to the country- an estimated $272 million in 1965. Some 2.75 million ft of hole was drilled in 1964, resulting in 97 oil fields and 4 major basins. Lost circulation has been a problem Transportation and communication are difficult in the vastmore » Libyan desert. Water-contaminated crude and high salt content have plagued production. These problems are being solved. With 45 drillings rigs active, crude production is expected to reach 2 million bpd by 1966. Most of the rigs used are trailer-mounted, unitized 8,000-12,000 ft capacity, that have been specially adapted to work in the Sahara. The drilling-completion programs are briefly described.« less

  19. PREFACE: Scientific and Technical Challenges in the Well Drilling Progress

    NASA Astrophysics Data System (ADS)

    2015-02-01

    The Conference "Advanced Engineering Problems in Drilling" was devoted to the 60th anniversary of the Drilling Department, Institute of Natural Resources. Today this Department is the "descendant" of two existing departments - Mining Exploration Technology and Oil and Gas Drilling. It should be mentioned that this remarkable date is associated with the first graduation class of mining engineers in "Mining Exploration Technologies", as well as the 30th anniversary of the Oil and Gas Well Drilling Department. Anniversary is an excellent occasion to remember one's historical past. At the beginning of the last century within the Tomsk Technological Institute n.a. Emperor Nikolai II the Mining Department was established which soon embraced the Obruchev-Usov Mining-Geological School. This School became the parent of mining-geological education in the Asian region of Russia, as well as the successor of mining-geological science. It was and is today one of the leading schools in the spheres of mineral resources exploration, surveying and mining. 1927 is the year of the establishment of the Department of Technology in Mineral Exploration. SibGeokom (Western-Siberia branch of the Geological Committee) under the supervision of M.A. Usov obtained the first Krelis rotary boring drill. Prior to that only the Keystone cable drilling rig was used in exploration. It was I.A. Molchanov who was responsible for the development and implementation of new technology in the field of exploration. In the yard of SibGeokom (now it is Building № 6, Usov St.) the first drilling rig was mounted. This was the beginning of the first training courses for Krelis drilling foremen under the supervision of I.A. Molchanov. In 1931 I.A. Molchanov headed the Department of Exploration which was located in Building № 6. In the outside territory of this building a drilling site was launched, including Keystone cable drilling rig, CAM-500 drilling rig and others. In the Building itself, i.e. in one study room (now № 107), the floor was lowered to 2 m and a drilling rig was mounted where students could obtain practical skills in drilling. The Department of Exploration became the foundation of the future department of Mining Exploration Technologies. However, the Department of Exploration, headed by A.A. Belitshky from 1944, furthered its work in the research sphere of drilling (including such leading specialists as P.F. Palyanov, V.I. Molchanov, I.S. Mitushkin, V.M. Matrosov, V.P. Krendelev) and in 1949 a new speciality was introduced "Technologies in Mineral Exploration." In 1952 the graduate of Moscow Geological Institute (now Geological Institute, Russian Academy of Science) PhD. S.S. Sulakshin began working in this Department, and in 1954 headed the newly established Department of Technologies in Mineral Exploration and was its continuous Head for more than 32 years and during the last 60 years has been a close associate of this department. Due to his brilliant supervision, this Department flourished and during the last 20 years has been one of the top departments in Russian affliated departments. In 1962 within the framework of this Department a new speciality was introduced - Oil and Gas Well Drilling. 125 full-time and 50 part-time students were enrolled in the two above-mentioned specialities. As a result, there was a necessity to open a new independent department which was in 1984 (October 1). The Department of Oil and Gas Well Drilling was located in Building № 8 and then in Building № 15. The Department staff included graduates of the Department of Technologies in Mineral Exploration and was headed by Yu. L. Boyarko, one of the first graduates of this Department. Time passed by and life made its own adjustments which influenced the further existence of these two departments. Due to the decrease of exploration and development drilling scope the student enrollment in the two above-mentioned specialties also decreased many-fold. As a result the two departments - Technologies in Mineral Exploration and Technologies in Mineral Exploration were merged into one department. In 2003 the newly merged Department of Drilling was established within the Institute of Petroleum Engineering, now the Institute of Natural Resources and is located in Building № 6 where it began its life. During these 60 years more than 3000 specialists have graduated the Department of Drilling, many whom are highly-qualified and dedicated professionals. There is no doubt that this Conference involved comprehensive advanced engineering problems in drilling and issues on relevant personnel training. It is extremely important to understand how the 60-year progress and contribution in the field of drilling has left its trace in the history of this Department; and, that, now, it is necessary to move further and seek new and new horizons in drilling.

  20. Mini-CORK observatories using the MeBo seafloor drill rig - a new development for long-term data acquisition and sampling in shallow boreholes

    NASA Astrophysics Data System (ADS)

    Kopf, A.; Freudenthal, T.; Ratmeyer, V.; Bergenthal, M.; Renken, J.; Zabel, M.; Wefer, G.

    2011-12-01

    State of the art technology for long-term monitoring of fluid migration within the sea floor is the sealing of a borehole with a Circulation Obviation Retrofit Kit (CORK) after sensor installation and/or fluid sampling devices within the drill string. However, the combined used of a drilling vessels and a remotely operated drilling (ROV) required for a CORK installation in the deep sea is a costly exercise that limits the number of monitoring stations installed. Robotic sea floor drill rigs are a cost effective alternative for shallow drillings down to 50-100 m below sea floor. Here we present a Mini-CORK system that is developed for installation with the sea floor drill rig MeBo. This rig was developed at MARUM Research Centre, University of Bremen in 2005 and can sample the sea floor in water depths up to 2000 m. The MeBo is deployed on the seabed and remotely controlled from the vessel. All required drill tools for wire-line core drilling down to 70 m below sea floor are stored on two rotating magazines and can be loaded below the top drive drill head for assembling the drill string. For one of the upcoming cruises with RV Sonne offshore Japan (Nankai Trough accretionary prism), MeBo will be used for the first time to place observatories. Two different designs have been developed. The first, relatively simple long-term device resembles a MeBo drill rod in its geometry, and contains a pressure and temperature transducer in the borehole plus an identical pair of transducers for seafloor reference. The device also contains a data logger, battery unit, and an acoustic modem so that data can be downloaded at any time from a ship of opportunity. The key element at the base of the observatory rod is a seal at the conical thread to separate the borehole hydraulically from the overlying water body. It is realized by an adapter, which also contains a hotstab hydraulic connection and an electrical connection. The second observatory device is a seafloor unit, which replaces part of the first unit and which is deployed by ROV. In essence, the upper portion of the former observatory is taken away by ROV, and an umbilical containing hydraulic lines and tubing to withdraw formation water from the borehole is plugged into the hotstab female adapter by ROV. At the far end, the umbilical is connected to a seafloor unit with battery power, data logger, P and T transducers, and the same acoustic modem as the former one. In addition, the latter contains osmo samplers and biological chambers (FLOCS) for in situ sampling and experiments. After the envisaged deployment period, the entire unit is replaced while an identical one is prepared on deck and lowered from the vessel. In theory, the MeBo hole infinitely serves as an access to depth since no electronic, but only tubing is lowered into the (open) hole. In summary, long-term borehole installations with MeBo offer an affordable way to measure key physical properties over time and sample the formation fluids for geochemistry and microbiology (in case of the second, ROV-deployed CORK).

  1. An automated tool joint inspection device for the drill string

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Moyer, M.C.; Dale, B.A.; Kusenberger, F.N.

    1983-02-01

    This paper discusses the development of an automated tool joint inspection device (i.e., the Fatigue Crack Detector), which is capable of detecting defects in the threaded region of drill pipe and drill collars. On the basis of inspection tests conducted at a research test facility and at drilling rig sites, this device is capable of detecting both simulated defects (saw slots and drilled holes) and service-induced defects, such as fatigue cracks, pin stretch (plastic deformation), mashed threads, and corrosion pitting. The system employs an electromagnetic flux-leakage principle and has several advantages over the conventional method of magnetic particle inspection.

  2. Results of an adaptive environmental assessment modeling workshop concerning potential impacts of drilling muds and cuttings on the marine environment

    USGS Publications Warehouse

    Auble, Gregor T.; Andrews, Austin K.; Ellison, Richard A.; Hamilton, David B.; Johnson, Richard A.; Roelle, James E.; Marmorek, David R.

    1983-01-01

    Drilling fluids or "muds" are essential components of modern drilling operations. They provide integrity for the well bore, a medium for removal of formation cuttings, and lubrication and cooling of the drill bit and pipe. The modeling workshop described in this report was conducted September 14-18, 1981 in Gulf Breeze, Florida to consider potential impacts of discharged drilling muds and cuttings on the marine environment. The broad goals of the workshop were synthesis of information on fate and effects, identification of general relationships between drilling fluids and the marine environment, and identification of site-specific variables likely to determine impacts of drilling muds and cuttings in various marine sites. The workshop was structured around construction of a model simulating fate and effects of discharges from a single rig into open water areas of the Gulf of Mexico, and discussion of factors that might produce different fate and effects in enclosed areas such as bays and estuaries. The simulation model was composed of four connected submodels. A Discharge/Fate submodel dealt with the discharge characteristics of the rig and the subsequent fate of discharged material. Three effects submodels then calculated biological responses at distances away from the rig for the water column, soft bottom benthos (assuming the rig was located over a soft bottom environment), and hard bottom benthos (assuming the rig was located over a hard bottom environment). The model focused on direct linkages between the discharge and various organisms rather than on how the marine ecosystem itself is interconnected. Behavior of the simulation model indicated relatively localized effects of drilling muds and cuttings discharged from a single platform into open water areas. Water column fate and effects were dominated by rapid dilution. Effects from deposition of spent mud and cuttings were spatially limited with relatively rapid recovery, especially in soft bottom benthic communities which were conceptualized as being adapted to frequent storms. This behavior was generated by the set of assumptions about linkages and functional relationships used to construct the model. Areas of uncertainty included methods for extrapolating 96-hr LC50 so results to exposures of varying lengths and concentrations; recovery rates of benthic communities; responses to various depths and rates of burial; fate and effects of the plume in relationship to stratification layers; and long-term and sub-lethal effects of slightly elevated concentrations of discharged materials. Evaluation of the assumptions of the Soft Bottom Submodel suggest that the assumptions used may have been relatively liberal estimates of resiliency of these communities. Discussion of "closed" water bodies such as bays and estuaries indicated several reasons to expect different and more complex fate and effects behavior in these areas. These factors included different species and communities (such as aquatic macrophytes and oyster beds), more complex circulation and stratification patterns, and potentially more active resuspension processes. Much of the possible difference in behavior in these areas centers around the extent to which they are “closed” or in the relative residence times of water and sediments in these areas as they determine the long-term dispersion of discharged material. Despite the complexity and variability of these areas, a large body of knowledge (such as that concerning fate and physical effects of dredge spoil) that could be effectively employed in analysis of potential fate and physical effects in enclosed areas was identified.

  3. 46 CFR 90.10-16 - Industrial vessel.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ventures. Included in this classification are such vessels as drill rigs, missile range ships, dredges... classification are vessels carrying freight for hire or engaged in oceanography, limnology, or the fishing...

  4. 46 CFR 90.10-16 - Industrial vessel.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ventures. Included in this classification are such vessels as drill rigs, missile range ships, dredges... classification are vessels carrying freight for hire or engaged in oceanography, limnology, or the fishing...

  5. 46 CFR 90.10-16 - Industrial vessel.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ventures. Included in this classification are such vessels as drill rigs, missile range ships, dredges... classification are vessels carrying freight for hire or engaged in oceanography, limnology, or the fishing...

  6. 46 CFR 90.10-16 - Industrial vessel.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ventures. Included in this classification are such vessels as drill rigs, missile range ships, dredges... classification are vessels carrying freight for hire or engaged in oceanography, limnology, or the fishing...

  7. 46 CFR 90.10-16 - Industrial vessel.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ventures. Included in this classification are such vessels as drill rigs, missile range ships, dredges... classification are vessels carrying freight for hire or engaged in oceanography, limnology, or the fishing...

  8. AURORA BOREALIS: a polar-dedicated European Research Platform

    NASA Astrophysics Data System (ADS)

    Wolff-Boenisch, Bonnie; Egerton, Paul; Thiede, Joern; Roberto, Azzolini; Lembke-Jene, Lester

    2010-05-01

    Polar research and in particular the properties of northern and southern high latitude oceans are currently a subject of intense scientific debate and investigations, because they are subject to rapid and dramatic climatic variations. Polar regions react more rapidly and intensively to global change than other regions of the earth. A shrinking of the Arctic sea-ice cover, potentially leading to an opening of sea passages to the north of North America and Eurasia, on the long to a "blue" Arctic Ocean would additionally have a strong impact on transport, commerce and tourism bearing potential risk for humans and complex ecosystems in the future. In spite of their critical role processes and feedbacks, especially in winter but not exclusively, are virtually unknown: The Arctic Ocean for example, it is the only basin of the world's oceans that has essentially not been sampled by the drill ships of the Deep-Sea Drilling Project (DSDP) or the Ocean Drilling Program (ODP) and its long-term environmental history and tectonic structure is therefore poorly known. Exceptions are the ODP Leg 151 and the more recent very successful ACEX-expedition of the Integrated Ocean Drilling Program (IODP) in 2004. To help to address the most pressing questions regarding climate change and related processes, a Pan-European initiative in the field of Earth system science has been put in place: AURORA BOREALIS is the largest environmental research infrastructure on the ESFRI roadmap of the European Community. AURORA BOREALIS is a very powerful research icebreaker, which will enable year-round operations in the Arctic and the Antarctic as well as in the adjacent ocean basins. Equipped with its drilling rig, the vessel is also capable to explore the presently completely unknown Arctic deep-sea floor. Last but not least, the ship is a floating observatory and mobile monitoring platform that permits to measure on a long-term basis comprehensive time series in all research fields relevant to global climate change. Chances and challenges rest in securing the construction and operation costs that need a dedicated consortium of interested countries and institutions to help tackling the biggest challenges of the next decades.

  9. 30 CFR 250.433 - What are the diverter actuation and testing requirements?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations...-control systems and control stations. You must also flow-test the vent lines. (a) For drilling operations... must conduct subsequent pressure tests within 7 days after the previous test. (b) For floating drilling...

  10. 30 CFR 250.431 - What are the diverter design and installation requirements?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations... floating drilling operations; (b) Use dual diverter lines arranged to provide for downwind diversion capability; (c) Use at least two diverter control stations. One station must be on the drilling floor. The...

  11. KSC-02pd1679

    NASA Image and Video Library

    2002-11-07

    KENNEDY SPACE CENTER, FLA. - A touring group of national and international participants look over some Precision Sampling's drilling rig, new equipment being used for environmental cleanup at Launch Complex 34-A, Cape Canaveral Spaceport.

  12. Hard-rock jetting. Part 2. Rock type decides jetting economics

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pols, A.C.

    1977-02-07

    In Part 2, Koninklijke Shell Exploratie en Produktie Laboratorium presents the results of jet-drilling laminated formations. Shell concludes that (1) hard, laminated rock cannot be jet-drilled satisfactorily without additional mechanical cutting aids, (2) the increase in penetration rate with bit-pressure drop is much lower for impermeable rock than it is for permeable rock, (3) drilling mud can have either a positive or a negative effect on penetration rate in comparison with water, depending on the material drilled, and (4) hard, isotropic, sedimentary, impermeable rock can be drilled using jets at higher rates than with conventional means. However, jetting becomes profitablemore » only in the case of expensive rigs.« less

  13. A Comparison of Pre-Test Means from Oil Field Workers Having Available Manuals and Those Lacking Manuals before Announced Training.

    ERIC Educational Resources Information Center

    Zambon, Franco

    A study assessed the validity of the hypothesis that offshore drilling personnel would independently study important material if they knew that they would eventually receive formal training on the topic covered in the material. Eighty-one crew members on an offshore drilling rig were randomly divided into experimental and control groups. Those in…

  14. Installation Restoration Program Phase 1 - Records Search, O’Hare Air Reserve Forces Facility, Illinois

    DTIC Science & Technology

    1983-12-01

    clarifiers, activated sludge units, trickling filters, aerobic and anaerobic digesters, and various dowatering devices and recommendations for...for locations of water- bearing fractures. Additional responsibilities included drilling with mud and air rotary drilling rigs as well as bucket auger...interpretation. Also conducted earth resistivity surveys in Georgia and Alabama Piedmont Provinces for locations of water- bearing fractures. Additional

  15. Confronting Space Debris: Strategies and Warnings from Comparable Examples Including Deepwater Horizon

    DTIC Science & Technology

    2010-01-01

    Horizon (DH) was an ultra deepwater , semisubmers- ible offshore drilling rig contracted to BP by its owner, Transocean. The rig was capable of...Warnings from Comparable Examples Including Deepwater Horizon 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT...research quality and objectivity. StrategieS and WarningS from Comparable exampleS inCluding deepWater Horizon Confronting SpaCe DebriS dave baiocchi

  16. APL-UW Deep Water Propagation: Philippine Sea Signal Physics and North Pacific Ambient Noise

    DTIC Science & Technology

    2015-10-15

    prompted an expansion of efforts to extract energy from ocean resources. Oil drilling is a well-known example, and recent projects include wind farms and...tidal turbines. The VLF sound contributions from these initiatives may be space/time limited (as with pile driving for wind farm towers or oil rig...decommissioning) or chronic (as with routine service vessel transportation to and from existing oil rigs). Additionally, the contributions are generally

  17. Noise characterization of oil and gas operations.

    PubMed

    Radtke, Cameron; Autenrieth, Daniel A; Lipsey, Tiffany; Brazile, William J

    2017-08-01

    In cooperation with The Colorado Oil and Gas Conservation Commission, researchers at Colorado State University performed area noise monitoring at 23 oil and gas sites throughout Northern Colorado. The goals of this study were to: (1) measure and compare the noise levels for the different phases of oil and gas development sites; (2) evaluate the effectiveness of noise barriers; and (3) determine if noise levels exceeded the Colorado Oil and Gas Conservation Commission noise limits. The four phases of oil and gas development include drilling, hydraulic fracturing, completion and production. Noise measurements were collected using the A- and C-weighted sound scales. Octave band analysis was also performed to characterize the frequency spectra of the noise measurements.  Noise measurements were collected using noise dosimeters and a hand-held sound-level meter at specified distances from the development sites in each cardinal direction. At 350 ft (107 m), drilling, hydraulic fracturing, and completion sites without noise barriers exceeded the maximum permissible noise levels for residential and commercial zones (55 dBA and 60 dBA, respectively). In addition, drilling and hydraulic fracturing sites with noise barriers exceeded the maximum permissible noise level for residential zones (55 dBA). However, during drilling, hydraulic fracturing, and completion operations, oil producers are allowed an exception to the noise permissible limits in that they only must comply with the industrial noise limit (80 dBA). It is stated in Rule 604.c.(2)A. that: "Operations involving pipeline or gas facility installation or maintenance, the use of a drilling rig, completion rig, workover rig, or stimulation is subject to the maximum permissible noise levels for industrial zones (80dBA)." [8] Production sites were within the Colorado Oil and Gas Conservation Commission permissible noise level criteria for all zones. At 350 ft (107 m) from the noise source, all drilling, hydraulic fracturing, and completion sites exceeded 65 dBC.  Current noise wall mitigation strategies reduced noise levels in both the A- and C-weighted scale measurements. However, this reduction in noise was not sufficient to reduce the noise below the residential permissible noise level (55 dBA).

  18. Exergetic analysis of autonomous power complex for drilling rig

    NASA Astrophysics Data System (ADS)

    Lebedev, V. A.; Karabuta, V. S.

    2017-10-01

    The article considers the issue of increasing the energy efficiency of power equipment of the drilling rig. At present diverse types of power plants are used in power supply systems. When designing and choosing a power plant, one of the main criteria is its energy efficiency. The main indicator in this case is the effective efficiency factor calculated by the method of thermal balances. In the article, it is suggested to use the exergy method to determine energy efficiency, which allows to perform estimations of the thermodynamic perfection degree of the system by the example of a gas turbine plant: relative estimation (exergetic efficiency factor) and an absolute estimation. An exergetic analysis of the gas turbine plant operating in a simple scheme was carried out using the program WaterSteamPro. Exergy losses in equipment elements are calculated.

  19. Sea Spray and Icing in the Emerging Open Water of the Arctic Ocean

    DTIC Science & Technology

    2015-06-12

    concentrations of wind-generated sea spray and the resulting spray icing on offshore structures, such as wind turbines and exploration, drilling , and...We anticipate that structures placed in shallow water—wind turbines, drilling rigs, or man-made production islands, for instance—will, therefore...and the severity of sea spray icing on fixed offshore structures. We will use existing information on the relationship of the spray concentration

  20. Subterranean drilling and in situ treatment of wastes using a contamination control system and methods relating thereto

    DOEpatents

    Jessmore, James J.; Loomis, Guy G.; Pettet, Mark C.; Flyckt, Melissa C.

    2004-09-28

    Systems and methods relating to subterranean drilling while maintaining containment of any contaminants released during the drilling. A thrust block installed over a zone of interest provides an overflow space for retaining any contaminants and excess sealant returns. Negative air pressure may be maintained in the overflow space by a ventilation system. Access ports in the thrust block seal the overflow space from the surrounding environment with a membrane seal. A flexible sack seal in the access port may be connected to a drill shroud prior to drilling, providing containment during drilling after the drill bit penetrates the membrane seal. The drill shroud may be adapted to any industry standard drilling rig and includes a connection conduit for connecting to the flexible sack seal and a flexible enclosure surrounding the drill shaft and of a length to accommodate full extension thereof. Upon withdrawal, the sack seal may be closed off and separated, maintaining containment of the overflow space and the drill shroud.

  1. 76 FR 62817 - National Offshore Safety Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... Joint Investigation Team for DEEPWATER HORIZON drilling rig explosion and sinking. (9) Update from the... Advisory Committee>NOSAC and then use the event key. The meeting will be recorded by a court reporter. A...

  2. 46 CFR 176.404 - Subsequent inspections for certification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., and on a sailing vessel, rigging and sails. The owner or operator must conduct all tests as required by the OCMI, and make the vessel available for all specific inspections and drills required by...

  3. Review-Esso Resources Canada Ltd. , Norman Wells expansion project drilling program

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Schaef, D.G.

    Esso Resources Canada Limited has embarked on a project to increase production from its Norman Wells Oil field located 145 km south of the Arctic Circle, from 475 m/sup 3//D to 4000 m/sup 3//D of crude oil. This paper provides details on the development drilling portion of the project which is comprised of 150 wells to be drilled in 3 years utilizing 2 drilling rigs from July 1982 through September 1985. The majority of the wells will be directionally drilled from multiwell land pads and artificial islands to shallow reservoir targets underlying the Mackenzie River, a major river intersecting themore » field boundaries. Experience from the initial 27 wells completed is provided.« less

  4. An outbreak of gastroenteritis on board an oil drilling rig in Ghana.

    PubMed

    Babayara, Michael N K; Addo, Bright

    2018-01-01

    The article describes an outbreak of gastroenteritis which occurred on an oil drilling rig which operated in the Jubilee fields of Ghana (offshore Takoradi) between May 2011 and December 2013. An outbreak occurred on board approximately 10 h following a Christmas day buffet. Various Ghanaian and continental dishes were served during the occasion. Over a 3 day period, a total of 33 cases were recorded at the rig's sick bay. In order to describe the outbreak and determine its possible association with the meals served at the buffet, this case control study was conducted. A short questionnaire was used for data collection and a line list prepared for summarising data of cases. Samples of food could not be taken but water samples from multiple points of the rig's pipe water system were collected and cultured for coliforms. Data was analysed using Epi info version 7. Six foods appeared to be associated with the outbreak with odds ratios ranging from 1.2 to 2.7. The epidemic curve showed a propagated fashion of transmission and the outbreak was brought under control with public education on hand washing and disinfection of surfaces and door handles. The conclusion was that food items served at the buffet were not associated with the outbreak. Instead, a possible contamination of surfaces and door handles probably caused it. To prevent future occurrences, routine and regular hand washing and disinfection of surfaces and door handles is recommended.

  5. Field calibration of dutch cone penetrometers for Louisiana soils : final report.

    DOT National Transportation Integrated Search

    1983-12-01

    Various sites that were penetrated by Louisiana's Department of Transportation and Development (DOTD) foundatioon boring crews with the mechanical cone penetrometer (Dutch Cone Penetrometer) and rotary drill rig (core borings) are discussed. A compar...

  6. Design and deployment of autoclave pressure vessels for the portable deep-sea drill rig MeBo (Meeresboden-Bohrgerät)

    NASA Astrophysics Data System (ADS)

    Pape, Thomas; Hohnberg, Hans-Jürgen; Wunsch, David; Anders, Erik; Freudenthal, Tim; Huhn, Katrin; Bohrmann, Gerhard

    2017-11-01

    Pressure barrels for sampling and preservation of submarine sediments under in situ pressure with the robotic sea-floor drill rig MeBo (Meeresboden-Bohrgerät) housed at the MARUM (Bremen, Germany) were developed. Deployments of the so-called MDP (MeBo pressure vessel) during two offshore expeditions off New Zealand and off Spitsbergen, Norway, resulted in the recovery of sediment cores with pressure stages equaling in situ hydrostatic pressure. While initially designed for the quantification of gas and gas-hydrate contents in submarine sediments, the MDP also allows for analysis of the sediments under in situ pressure with methods typically applied by researchers from other scientific fields (geotechnics, sedimentology, microbiology, etc.). Here we report on the design and operational procedure of the MDP and demonstrate full functionality by presenting the first results from pressure-core degassing and molecular gas analysis.

  7. Scanning the Horizon: Coast Guard Strategy in a Hot, Flat, Crowded World

    DTIC Science & Technology

    2010-03-12

    Mexico. From 1992 to 2007, deepwater offshore rigs drilling in deep water in the Gulf of Mexico increased from three to 30, and deepwater oil production...discusses the Coast Guard’s Integrated Deepwater System program, which includes recapitalization of its deep-water vessels and aircraft.89 At the...water and ultra deep water drilling. Discussion of increased outer continental shelf activity in higher level strategic planning indicates that

  8. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    This paper is actually a composite of two papers dealing with automation and computerized control of underground mining equipment. The paper primarily discusses drills, haulage equipment, and tunneling machines. It compares performance and cost benefits of conventional equipment to the new automated methods. The company involved are iron ore mining companies in Scandinavia. The papers also discusses the different equipment using air power, water power, hydraulic power, and computer power. The different drill rigs are compared for performance and cost.

  9. An automated tool-joint inspection device for the drillstring

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Moyer, M.C.; Dale, B.A.; Kusenberger, F.N.

    1984-06-01

    This paper discusses the development of an automated tool joint inspection device-i.e., the fatigue crack detector (FCD), which can detect defects in the threaded region of drillpipe and drill collars. Inspection tests conducted at a research test facility and at drilling rig sites indicate that this device can detect both simulated defects (saw slots and drilled holes) and service-induced defects, such as fatigue cracks, pin stretch (plastic deformation), mashed threads, and corrosion pitting. The system operates on an electromagnetic-flux leakage principle and has several advantages over the conventional method of magnetic particle inspection.

  10. 78 FR 58989 - Electrical Equipment in Hazardous Locations; Extension of Comment Period

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-25

    ... to foreign Mobile Offshore Drilling Units (MODUs), floating facilities, and vessels that engage in... offshore supply vessels (OSVs). The proposed regulations would expand the list of national and... Purpose On September 6, 2013, we received a letter from the International Association of Drilling...

  11. Conoco's new approach to drill site construction in difficult, remote, swamp and jungle terrain Irian Jaya, Indonesia

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Roodriguez, F.H.

    1984-02-01

    In October 1982, Conoco Irian Jaya as operator: and partners: Pertamina, Inpex Bintuni Limited, and Moeco Irian Jaya Company, mobilized construction equipment from Singapore to the KBS ''A'' contract area in Irian Jaya, Indonesia for the purpose of constructing a base camp and drill three exploratory sites. What made this construction effort different from others previously used in Irian Jaya; was that it incorporated several new and unique features, namely: a turnkey approach to construction; that is Conoco providing complete set of specifications and conditions with contractor assuming risks for a lump sum payment; special equipment designed by contractor formore » Irian Jaya operations; an incentive to co pensate or penalize contractor for helicopter hours flown below or above a predetermined number; structural steel pile platform designs for two swamp locations (Ayot and Aum), as opposed to the more conventional corduroy timber log-plank arrangement; and drilling rig pads designed for specific heli-rig with limited extra space. All work was successfully completed within the time frame stipulated in the contract, that is five months from the time the contractor was notified to begin mobilization of equipment, materials and personnel.« less

  12. Research of frequency converters energy characteristics of drilling rigs

    NASA Astrophysics Data System (ADS)

    Vasiliev, B. Y.; Kalashnikov, O. V.; Oleynikova, A. M.; Ivanovsky, A. I.; Grudinin, N. N.

    2017-10-01

    The investigation deals with multi-motor electric drives with frequency converters of various structures: with a common converter, with an individual converter, with a multi-inverter frequency converter. Their shortcomings and advantages were analyzed and there were drawn conclusions about the expediency of using each structure. Expediency of using multi-inverter frequency converters with an active frond end was shown to ensure the highest power characteristics of multi-motor electric drives of drilling rigs’ main mechanisms.

  13. USSR Report: Earth Sciences. No. 26

    DTIC Science & Technology

    1983-06-07

    supplied with an automated drill rig with a great load- lifting capacity, screws for self-movement, and also an automated anchorless system for holding...is compensated by the Archimedes force acting from the direction of the denser subcrustal matter — the substrate. A disrup- tion of this equilibrium...held on the lower end of a column of drilling pipes [7] which can be rotated by means of a drive pipe [8] imparted a torque by the rotor. In

  14. Indonesian drilling maintains steady pace

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1985-05-01

    Offshore drilling activity in Indonesia increased nominally the first quarter of 1985 to an average 29 rigs. Barring any further problems with oil prices and markets, operators are expected to maintain essentially the current general level of appraisal/development work for the rest of this year. There are still a number of prospective regions to be explored in Southeast Asia. Regional developments are described for the South China Sea area, the Java Sea, South Sumatra, Kalimantan, Irian Jaya and the Malacca Strait.

  15. Belukha whale (delphinapterus leucas) responses to industrial noise in Nushagak Bay, Alaska: 1983

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stewart, B.S.; Awbrey, F.T.; Evans, W.E.

    1983-01-01

    Between 15 June and 14 July 1983 the authors conducted playback experiments with belukha whales in the Snake River, Alaska, using sounds recorded near an operating oil-drilling rig. The objectives of these experiments were to quantify behavioral responses of belukha whales to oil drilling noise in an area where foreign acoustic stimuli were absent, and to test the hypothesis that beluhka whales would not approach a source of loud sound.

  16. Summary of regional geology, petroleum potential, resource assessment and environmental considerations for oil and gas lease sale area No. 56

    USGS Publications Warehouse

    Dillon, William P.; Klitgord, Kim D.; Paull, Charles K.; Grow, John A.; Ball, Mahlon M.; Dolton, Gordon L.; Powers, Richard B.; Khan, Abdul S.; Popenoe, Peter; Robb, James M.; Dillon, William P.

    1980-01-01

    This report summarizes our general knowledge of the petroleum potential, as well as problems and hazards associated with development of petroleum resources in the area proposed for nominations for lease sale number 56. This area includes the U.S. eastern continental margin from the North Carolina-Virginia border south to approximately Cape Canaveral, Florida and from three miles from shore, seaward to include the upper Continental Slope and inner Blake Plateau. The area for possible sales is shown in figure 1; major physiographic features of the region are shown in figure 2.No wells have been drilled for petroleum within this proposed lease area and no significant commercial production has been obtained onshore in the Southeast Georgia Embayment. The COST GE-1 stratigraphic test well, drilled on the Continental Shelf off Jacksonville, Fla. (fig- 1), reached basement at 3,300 m. The bottom third of the section consists of dominantly continental rocks that are typically poor sources of petroleum (Scholle, 1979) and the rocks that contain organic carbon adequate for generation of petroleum at the well are seen in seismic profiles always at shallow subbottom depths, so they probably have not reached thermal maturity. However, seismic profiles indicate that the sedimentary deposits thicken markedly in a seaward direction where more of the section was deposited under marine conditions; therefore, commercial accumulations of petroleum offshore are more likely.Several potential sources of environmental hazard exist. Among the most important are hurricanes, the Gulf Stream, and earthquakes. The potential danger from high wind, waves, storm surges, and storm-driven currents associated with hurricanes is obvious. Evidence for significant bottom scour by the Gulf Stream is abundant; such scour is a threat to the stability of bottom-mounted structures. The fast-flowing water also will hamper floating drill rigs and control of drill strings. A major earthquake of about magnitude 6.8 struck Charleston in 1886; it may have been associated with a zone of active seismicity that crosses South Carolina. The likelihood of a repetition of the 1886 event is presently not predictable but a seismic hazard must be assumed to exist.

  17. 30 CFR 250.1911 - What criteria for hazards analyses must my SEMS program meet?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER... to the seabed (i.e., mobile offshore drilling units; floating production systems; floating production..., production, and transportation activities for oil, gas, or sulphur from areas leased in the OCS. Facilities...

  18. 30 CFR 250.1911 - What criteria for hazards analyses must my SEMS program meet?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Safety...., mobile offshore drilling units; floating production systems; floating production, storage and offloading... transportation activities for oil, gas, or sulphur from areas leased in the OCS. Facilities also include DOI...

  19. 46 CFR 90.05-35 - Flammable and combustible liquid cargo in bulk.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Miscellaneous Vessels, such as cable, salvage, pile-driving, and oil-drilling-rig vessels: (1) Grades B, C, D...: Requirements for double hull construction for vessels carrying oil, as defined in 33 CFR 157.03, in bulk as...

  20. 46 CFR 90.05-35 - Flammable and combustible liquid cargo in bulk.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) Miscellaneous Vessels, such as cable, salvage, pile-driving, and oil-drilling-rig vessels: (1) Grades B, C, D...: Requirements for double hull construction for vessels carrying oil, as defined in 33 CFR 157.03, in bulk as...

  1. 46 CFR 90.05-35 - Flammable and combustible liquid cargo in bulk.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) Miscellaneous Vessels, such as cable, salvage, pile-driving, and oil-drilling-rig vessels: (1) Grades B, C, D...: Requirements for double hull construction for vessels carrying oil, as defined in 33 CFR 157.03, in bulk as...

  2. 46 CFR 90.05-35 - Flammable and combustible liquid cargo in bulk.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) Miscellaneous Vessels, such as cable, salvage, pile-driving, and oil-drilling-rig vessels: (1) Grades B, C, D...: Requirements for double hull construction for vessels carrying oil, as defined in 33 CFR 157.03, in bulk as...

  3. 46 CFR 90.05-35 - Flammable and combustible liquid cargo in bulk.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) Miscellaneous Vessels, such as cable, salvage, pile-driving, and oil-drilling-rig vessels: (1) Grades B, C, D...: Requirements for double hull construction for vessels carrying oil, as defined in 33 CFR 157.03, in bulk as...

  4. HIGH-POWER TURBODRILL AND DRILL BIT FOR DRILLING WITH COILED TUBING

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Robert Radtke; David Glowka; Man Mohan Rai

    2008-03-31

    Commercial introduction of Microhole Technology to the gas and oil drilling industry requires an effective downhole drive mechanism which operates efficiently at relatively high RPM and low bit weight for delivering efficient power to the special high RPM drill bit for ensuring both high penetration rate and long bit life. This project entails developing and testing a more efficient 2-7/8 in. diameter Turbodrill and a novel 4-1/8 in. diameter drill bit for drilling with coiled tubing. The high-power Turbodrill were developed to deliver efficient power, and the more durable drill bit employed high-temperature cutters that can more effectively drill hardmore » and abrasive rock. This project teams Schlumberger Smith Neyrfor and Smith Bits, and NASA AMES Research Center with Technology International, Inc (TII), to deliver a downhole, hydraulically-driven power unit, matched with a custom drill bit designed to drill 4-1/8 in. boreholes with a purpose-built coiled tubing rig. The U.S. Department of Energy National Energy Technology Laboratory has funded Technology International Inc. Houston, Texas to develop a higher power Turbodrill and drill bit for use in drilling with a coiled tubing unit. This project entails developing and testing an effective downhole drive mechanism and a novel drill bit for drilling 'microholes' with coiled tubing. The new higher power Turbodrill is shorter, delivers power more efficiently, operates at relatively high revolutions per minute, and requires low weight on bit. The more durable thermally stable diamond drill bit employs high-temperature TSP (thermally stable) diamond cutters that can more effectively drill hard and abrasive rock. Expectations are that widespread adoption of microhole technology could spawn a wave of 'infill development' drilling of wells spaced between existing wells, which could tap potentially billions of barrels of bypassed oil at shallow depths in mature producing areas. At the same time, microhole coiled tube drilling offers the opportunity to dramatically cut producers' exploration risk to a level comparable to that of drilling development wells. Together, such efforts hold great promise for economically recovering a sizeable portion of the estimated remaining shallow (less than 5,000 feet subsurface) oil resource in the United States. The DOE estimates this U.S. targeted shallow resource at 218 billion barrels. Furthermore, the smaller 'footprint' of the lightweight rigs utilized for microhole drilling and the accompanying reduced drilling waste disposal volumes offer the bonus of added environmental benefits. DOE analysis shows that microhole technology has the potential to cut exploratory drilling costs by at least a third and to slash development drilling costs in half.« less

  5. 30 CFR 250.431 - What are the diverter design and installation requirements?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.431 What are the diverter... wellhead configurations and at least 12 inches for floating drilling operations; (b) Use dual diverter...

  6. Proper nozzle location, bit profile, and cutter arrangement affect PDC-bit performance significantly

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Garcia-Gavito, D.; Azar, J.J.

    1994-09-01

    During the past 20 years, the drilling industry has looked to new technology to halt the exponentially increasing costs of drilling oil, gas, and geothermal wells. This technology includes bit design innovations to improve overall drilling performance and reduce drilling costs. These innovations include development of drag bits that use PDC cutters, also called PDC bits, to drill long, continuous intervals of soft to medium-hard formations more economically than conventional three-cone roller-cone bits. The cost advantage is the result of higher rates of penetration (ROP's) and longer bit life obtained with the PDC bits. An experimental study comparing the effectsmore » of polycrystalline-diamond-compact (PDC)-bit design features on the dynamic pressure distribution at the bit/rock interface was conducted on a full-scale drilling rig. Results showed that nozzle location, bit profile, and cutter arrangement are significant factors in PDC-bit performance.« less

  7. Redesigned PDC bit solves low hydraulic hp problems

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-06-01

    A new PDC bit design was created to solve a problem a drilling contractor had due to hydraulic horsepower limitations on rigs used in a particular geographical area. The new bit design, which arose from a formal alliance between Exeter Drilling Co. and Hughes Christensen Co. has greatly improved bit cleaning and overall drilling efficiency in applications where only low hydraulic hp is available. The new design has been run successfully in the Denver-Julesburg (D-J) basin of Colorado. The development was described in detail in paper IADC/SPE 35109, ``Unique PDC bit configuration dramatically improves hole cleaning, drilling efficiency in lowmore » hydraulic applications,`` presented by G.J. Hertzler III, Exeter Drilling Co. and J.T. Wankier, Hughes Christensen Co., at the 1996 IADC/SPE Drilling Conference, New Orleans, La., March 12--15. This article is an abstract of that paper, which contains significantly more technical data.« less

  8. Means of atmospheric air pollution reduction during drilling wells

    NASA Astrophysics Data System (ADS)

    Shkitsa, L.; Yatsyshyn, T.; Lyakh, M.; Sydorenko, O.

    2016-08-01

    The process of drilling oil and gas wells is the source of air pollution through drilling mud evaporation containing hazardous chemical substances. The constructive solution for cleaning device of downhole tool that contains elements covering tube and clean the surface from the mud in the process of rising from the well is offered. Inside the device is filled with magnetic fluid containing the substance neutralizing hazardous substances. The use of the equipment proposed will make it possible to avoid penetration of harmful substances into the environment and to escape the harmful effects of aggressive substances for staff health and increase rig's fire safety.

  9. Blowout Prevention System Events and Equipment Component Failures : 2016 SafeOCS Annual Report

    DOT National Transportation Integrated Search

    2017-09-22

    The SafeOCS 2016 Annual Report, produced by the Bureau of Transportation Statistics (BTS), summarizes blowout prevention (BOP) equipment failures on marine drilling rigs in the Outer Continental Shelf. It includes an analysis of equipment component f...

  10. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hadley, I.; Sinclair, C.I.K.; Magne, E.

    This paper describes the life extension of a semi-submersible drilling rig built in the early 1970`s. A nominal design life of 20 years was estimated at the time of building; however, in the interim period, numerous improvements have been made in fatigue life estimation ad life improvement techniques, raising the possibility that a further 20 years of operation could be considered. The life extension strategy made use of a number of aspects of offshore technology which were not available at the time of construction of the rig. Finite element studies and results from offshore research programs were used to gaugemore » the effect of fatigue life improvement techniques. The program demonstrated the feasibility of extending the operation of the rig for a further 20 years, with the interval between in-service inspection increased to every five years. It also provided a valuable database of fracture toughness data for the rig materials, which may be used in future work to address reliability issues.« less

  11. 30 CFR 250.431 - What are the diverter design and installation requirements?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.431 What are the diverter design and... configurations and at least 12 inches for floating drilling operations; (b) Use dual diverter lines arranged to...

  12. 30 CFR 250.431 - What are the diverter design and installation requirements?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.431 What are the diverter design and... configurations and at least 12 inches for floating drilling operations; (b) Use dual diverter lines arranged to...

  13. 30 CFR 250.431 - What are the diverter design and installation requirements?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.431 What are the diverter design and... configurations and at least 12 inches for floating drilling operations; (b) Use dual diverter lines arranged to...

  14. MeBo70 Seabed Drilling on a Polar Continental Shelf: Operational Report and Lessons From Drilling in the Amundsen Sea Embayment of West Antarctica

    NASA Astrophysics Data System (ADS)

    Gohl, K.; Freudenthal, T.; Hillenbrand, C.-D.; Klages, J.; Larter, R.; Bickert, T.; Bohaty, S.; Ehrmann, W.; Esper, O.; Frederichs, T.; Gebhardt, C.; Küssner, K.; Kuhn, G.; Pälike, H.; Ronge, T.; Simões Pereira, P.; Smith, J.; Uenzelmann-Neben, G.; van de Flierdt, C.

    2017-11-01

    A multibarrel seabed drill rig was used for the first time to drill unconsolidated sediments and consolidated sedimentary rocks from an Antarctic shelf with core recoveries between 7% and 76%. We deployed the MARUM-MeBo70 drill device at nine drill sites in the Amundsen Sea Embayment. Three sites were located on the inner shelf of Pine Island Bay from which soft sediments, presumably deposited at high sedimentation rates in isolated small basins, were recovered from drill depths of up to 36 m below seafloor. Six sites were located on the middle shelf of the eastern and western embayment. Drilling at five of these sites recovered consolidated sediments and sedimentary rocks from dipping strata spanning ages from Cretaceous to Miocene. This report describes the initial coring results, the challenges posed by drifting icebergs and sea ice, and technical issues related to deployment of the MeBo70. We also present recommendations for similar future drilling campaigns on polar continental shelves.

  15. Method of deep drilling

    DOEpatents

    Colgate, Stirling A.

    1984-01-01

    Deep drilling is facilitated by the following steps practiced separately or in any combination: (1) Periodically and sequentially fracturing zones adjacent the bottom of the bore hole with a thixotropic fastsetting fluid that is accepted into the fracture to overstress the zone, such fracturing and injection being periodic as a function of the progression of the drill. (2) Casing the bore hole with ductile, pre-annealed casing sections, each of which is run down through the previously set casing and swaged in situ to a diameter large enough to allow the next section to run down through it. (3) Drilling the bore hole using a drill string of a low density alloy and a high density drilling mud so that the drill string is partially floated.

  16. The Georges Bank monitoring program 1985; analysis of trace metals in bottom sediments during the third year of monitoring

    USGS Publications Warehouse

    Bothner, Michael H.; Rendigs, R. R.; Campbell, Esma; Doughten, M.W.; Parmenter, C.M.; O'Dell, C. H.; DiLisio, G.P.; Johnson, R.G.; Gillison, J.R.; Rait, Norma

    1986-01-01

    Of the 12 elements analyzed in bulk (undifferentiated) sediments collected adjacent to drilling rigs on Georges Bank, only barium was found to increase in concentration during the period when eight exploratory wells were drilled (July 1981 until September 1982). The maximum postdrilling concentration of barium (a major element in drilling mud) reached 172 ppm in bulk sediments near the drill site in block 410. This concentration is higher than the predrilling concentration at this location by a factor of 5.9. This maximum barium concentration is within the range of predrilling concentrations (28-300 ppm) measured in various sediment types from the regional stations of this program. No drilling-related changes in the concentrations of the 11 other metals have been observed in bulk sediments at any of the locations sampled in this program. We estimate that about 25 percent of the barite discharged at block 312 was present in the sediments within 6 km of the rig, 4 weeks after drilling was completed at this location (drilling period was December 8, 1981-June 27, 1982). For almost a year following completion of this well, the inventory of barite decreased rapidly, with a half-life of 0.34 year. During the next year, the inventory decreased at a slower rate (half-life of 3.4 years). The faster rate probably reflects resuspension and sediment transport of bariterich material residing at the sediment surface. Elevated barium concentrations in post-drilling sediment-trap samples from block 312 indicate that such resuspension can occur up to at least 25 m above the sea floor. As the remaining barite particles are reworked deeper into the sediments by currents and bioturbation, removal by sediment-transport processes is slower. The barite discharged during the exploratory phase of drilling is associated with the fine fraction of sediment and is widely distributed around the bank. We found evidence for barium transport to Great South Channel, 115 km west of the drilling, and to stations 2 and 3, 35 km east of the easternmost drilling site. Small increases in barium concentrations, present in the fine fraction of sediment only, were measured also at the heads of both Lydonia and Oceanographer Canyons, located 8 and 39 km, respectively, seaward of the nearest exploratory well.

  17. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wagner, B.

    A specially designed wire line retrievable continuous coring system cored its initial project wells to total depth in hard rock formations in less than half the time that would have been required by conventional coring rigs. The hybrid wire line coring systems have since been used on other wells in similar lithologies, with a total of 38,000 m (124,640 ft) of hole cored and with penetration rates averaging 2.27 m/hr (7.45 ft/hr). This paper reports that Parker Drilling Co. designed the hybrid rigs and has recently been contracted to wire line core several holes for oil and gas exploration inmore » the Congo. The first core hole has been completed to 1,490 m, and total depth was reached in 21 days. The rig is now being mobilized to a second hole in the Congo.« less

  18. Underwater noise from geotechnical drilling and standard penetration testing.

    PubMed

    Erbe, Christine; McPherson, Craig

    2017-09-01

    Geotechnical site investigations prior to marine construction typically involve shallow, small-core drilling and standard penetration testing (SPT), during which a small tube is hammered into the ground at the bottom of the borehole. Drilling (120 kW, 83 mm diameter drillbit, 1500 rpm, 16-17 m drill depth in sand and mudstone) and SPT (50 mm diameter test tube, 15 mm wall thickness, 100 kg hammer, 1 m drop height) by a jack-up rig in 7-13 m of water were recorded with a drifting hydrophone at 10-50 m range. Source levels were 142-145 dB re 1 μPa rms @ 1 m (30-2000 Hz) for drilling and 151-160 dB re 1 μPa 2 s @ 1 m (20-24 000 Hz) for SPT.

  19. Interior Department Suggests Improvements for Offshore Arctic Oil and Gas Drilling

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    2013-03-01

    Shell's "difficulties" during its 2012 program to drill offshore oil and natural gas exploration wells in the Alaskan Arctic Ocean "have raised serious questions regarding its ability to operate safely and responsibly in the challenging and unpredictable conditions offshore Alaska," according to the report "Review of Shell's 2012 Alaska Offshore Oil and Gas Exploration Program," issued by the U.S. Department of the Interior (DOI) on 8 March. Noting the company's lack of adequate preparation for drilling in the Arctic, its failure to deploy a specialized Arctic Containment System, and the grounding of the Kulluk drilling rig near Kodiak Island last December, the report recommends that Shell develop a comprehensive and integrated plan describing its future drilling program and related operations and that it commission a third-party audit of its management systems, including its safety and environmental management systems program.

  20. 30 CFR 250.432 - How do I obtain a departure to diverter design and installation requirements?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.432 How do I obtain a departure to diverter... diverter line for floating drilling operations on a dynamically positioned drillship Maintain an...

  1. 30 CFR 250.432 - How do I obtain a departure to diverter design and installation requirements?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... MANAGEMENT, REGULATION, AND ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250... diameter of at least 8 inches. (d) Use a single diverter line for floating drilling operations on a...

  2. 30 CFR 250.432 - How do I obtain a departure to diverter design and installation requirements?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.432 How do I obtain a... least 8 inches. (d) Use a single diverter line for floating drilling operations on a dynamically...

  3. 30 CFR 250.432 - How do I obtain a departure to diverter design and installation requirements?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.432 How do I obtain a... least 8 inches. (d) Use a single diverter line for floating drilling operations on a dynamically...

  4. 30 CFR 250.432 - How do I obtain a departure to diverter design and installation requirements?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Oil and Gas Drilling Operations Diverter System Requirements § 250.432 How do I obtain a... least 8 inches. (d) Use a single diverter line for floating drilling operations on a dynamically...

  5. Modeling of oil mist and oil vapor concentration in the shale shaker area on offshore drilling installations.

    PubMed

    Bråtveit, Magne; Steinsvåg, Kjersti; Lie, Stein Atle; Moen, Bente E

    2009-11-01

    The objective of this study was to develop regression models to predict concentrations of oil mist and oil vapor in the workplace atmosphere in the shale shaker area of offshore drilling installations. Collection of monitoring reports of oil mist and oil vapor in the mud handling areas of offshore drilling installations was done during visits to eight oil companies and five drilling contractors. A questionnaire was sent to the rig owners requesting information about technical design of the shaker area. Linear mixed-effects models were developed using concentration of oil mist or oil vapor measured by stationary sampling as dependent variables, drilling installation as random effect, and potential determinants related to process technical parameters and technical design of the shale shaker area as fixed effects. The dataset comprised stationary measurements of oil mist (n = 464) and oil vapor (n = 462) from the period 1998 to 2004. The arithmetic mean concentrations of oil mist and oil vapor were 3.89 mg/m(3) and 39.7 mg/m(3), respectively. The air concentration models including significant determinants such as viscosity of base oil, mud temperature, well section, type of rig, localization of shaker, mechanical air supply, air grids in outer wall, air curtain in front of shakers, and season explained 35% and 17% of the total variance in oil vapor and oil mist, respectively. The developed models could be used to indicate what impact differences in technical design and changes in process parameters have on air concentrations of oil mist and oil vapor. Thus, the models will be helpful in planning control measures to reduce the potential for occupational exposure.

  6. Development of an Ultra-Light Multipurpose Drill and Tooling for the Transportable Array in Alaska

    NASA Astrophysics Data System (ADS)

    Coyle, B. J.; Lundgren, M.; Busby, R. W.

    2014-12-01

    Over the next four years the EarthScope Transportable Array (TA) will install approximately 250 to 275 broadband seismic stations in Alaska and Western Canada. The station plans build on recent developments in posthole broadband seismometer design and call for sensors to be installed in boreholes 7 inches diameter, from 1 to 5 meters deep. These boreholes will be lined with PVC or steel casing, grouted in place. The proposed station locations are in a grid-like pattern with a nominal spacing of 85 km. Since most of these locations will only be accessible by helicopter, it was necessary to develop an ultra-light drilling system that could be transported to site in one sling load by a high performance light helicopter (i.e. AS350B2 or Bell 407) and still be able to drill the variety of ground conditions we expect to encounter. In the past year we have developed a working prototype, gasoline-hydraulic drill rig that can be configured to run auger, diamond core or DTH tools, and weighs <1,300 lbs, including tooling. We have successfully drilled over 30 boreholes with this drill, including 12 for TA installations in Alaska and 13 at the Piñon Flat Observatory for testing sensor performance and placement techniques. Our drilling solution comprises: - Hydraulic system using a variable flow pump with on-demand load sensing valves to reduce the engine size needed and to cut down on heat build-up; - Rotation head mounting system on the travelling block to enable quick change of drilling tools; - Low speed, high torque rotation head for the auger, and an anchoring system that enables us to apply up to 5,000 lbs downforce for augering in permafrost; - Custom DTH that can run on low air pressure and air flow, yet is still robust enough to drill a 7 inch hole 2.5 meters through solid rock; - One-trip casing advance drilling with the DTH, steel casing is loaded at the start of drilling and follows the drill bit down; - Grout-through bottom caps for sealing the borehole casing and cementing it in place. Our next step is to build a dedicated DTH drilling system that will be light enough to mobilize to sites in one helicopter sling, including an air compressor. This rig is currently on the drawing board and we expect to build it this winter for field testing in the spring.

  7. Translations on Latin America, Number 1657

    DTIC Science & Technology

    1977-06-01

    Text] The 17th of last March, Industria del Hierro , S.A. [Iron Industries] delivered through its mechanical division-8 land rigs for drilling...design of this equipment allows it to be re-installed in only 3 days. Industria del Hierro was established in the city of Queretaro in 1963, with

  8. 46 CFR 30.01-5 - Application of regulations-TB/ALL.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... this chapter. (3) Miscellaneous vessels, such as cable, salvage, pile-driving and oil-drilling-rig... vessels that carry oil. (a) The regulations in this subchapter contain requirements for materials, design... the navigable waters of the United States, or which transfers oil in any port or place subject to the...

  9. 46 CFR 30.01-5 - Application of regulations-TB/ALL.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... this chapter. (3) Miscellaneous vessels, such as cable, salvage, pile-driving and oil-drilling-rig... vessels that carry oil. (a) The regulations in this subchapter contain requirements for materials, design... the navigable waters of the United States, or which transfers oil in any port or place subject to the...

  10. 46 CFR 30.01-5 - Application of regulations-TB/ALL.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... this chapter. (3) Miscellaneous vessels, such as cable, salvage, pile-driving and oil-drilling-rig... vessels that carry oil. (a) The regulations in this subchapter contain requirements for materials, design... which transfers oil in any port or place subject to the jurisdiction of the United States, must comply...

  11. 46 CFR 30.01-5 - Application of regulations-TB/ALL.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... this chapter. (3) Miscellaneous vessels, such as cable, salvage, pile-driving and oil-drilling-rig... vessels that carry oil. (a) The regulations in this subchapter contain requirements for materials, design... the navigable waters of the United States, or which transfers oil in any port or place subject to the...

  12. Investigation of the potential effects of underwater noise from petroleum-industry activities on feeding humpback whale behavior. Final report

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Malme, C.I.; Miles, P.R.; Tyack, P.

    1985-06-01

    An investigation was made of the potential effects of underwater noise from petroleum-industry activities on the behavior of feeding humpback whales in Frederick Sound and Stephens Passage, Alaska in August, 1984. Test sounds were a 100 cu. in. air gun and playbacks of recorded drillship, drilling platform, production platform, semi-submersible drill rig, and helicopter fly-over noise. Sound source levels and acoustic propagation losses were measured. The movement patterns of whales were determined by observations of whale-surfacing positions.

  13. Downhole tool

    DOEpatents

    Hall, David R.; Muradov, Andrei; Pixton, David S.; Dahlgren, Scott Steven; Briscoe, Michael A.

    2007-03-20

    A double shouldered downhole tool connection comprises box and pin connections having mating threads intermediate mating primary and secondary shoulders. The connection further comprises a secondary shoulder component retained in the box connection intermediate a floating component and the primary shoulders. The secondary shoulder component and the pin connection cooperate to transfer a portion of makeup load to the box connection. The downhole tool may be selected from the group consisting of drill pipe, drill collars, production pipe, and reamers. The floating component may be selected from the group consisting of electronics modules, generators, gyroscopes, power sources, and stators. The secondary shoulder component may comprises an interface to the box connection selected from the group consisting of radial grooves, axial grooves, tapered grooves, radial protrusions, axial protrusions, tapered protrusions, shoulders, and threads.

  14. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Barker, Ben; Moore, Joe; Segall, Marylin

    The Tularosa-Hueco basin in south-central New Mexico has long been known as an extensional area of high heat flow. Much of the basin is within the Fort Bliss military reservation, which is an exceptionally high value customer for power independent of the regional electric grid and for direct use energy in building climate control. A series of slim holes drilled in the 1990s established the existence of a thermal anomaly but not its practical value. This study began in 2009 with a demonstration of new exploration drilling technology. The subsequent phases reported here delivered a useful well, comparative exploration datamore » sets and encouragement for further development. A production-size well, RMI56-5, was sited after extensive study of archival and newly collected data in 2010-2011. Most of 2012 was taken up with getting state and Federal authorities to agree on a lead agency for permitting purposes, getting a drilling permit and redesigning the drilling program to suit available equipment. In 2013 we drilled, logged and tested a 924 m well on the McGregor Range at Fort Bliss using a reverse circulation rig. Rig tests demonstrated commercial permeability and the well has a 7-inch slotted liner for use either in production or injection. An August 2013 survey of the completed well showed a temperature of 90 C with no reversal, the highest such temperature in the vicinity. The well’s proximity to demand suggests a potentially valuable resource for direct use heat and emergency power generation. The drilling produced cuttings of excellent size and quality. These were subjected to traditional analyses (thin sections, XRD) and to the QEMScan™ for comparison. QEMScan™ technology includes algorithms for determining such properties of rocks as density, mineralogy, heavy/light atoms, and porosity to be compared with direct measurements of the cuttings. In addition to a complete cuttings set, conventional and resistivity image logs were obtained in the open hole before the well was cased.« less

  15. Lunar deep drill apparatus

    NASA Technical Reports Server (NTRS)

    Harvey, Jill (Editor)

    1989-01-01

    A self contained, mobile drilling and coring system was designed to operate on the Lunar surface and be controlled remotely from earth. The system uses SKITTER (Spatial Kinematic Inertial Translatory Tripod Extremity Robot) as its foundation and produces Lunar core samples two meters long and fifty millimeters in diameter. The drill bit used for this is composed of 30 per carat diamonds in a sintered tungsten carbide matrix. To drill up to 50 m depths, the bit assembly will be attached to a drill string made from 2 m rods which will be carried in racks on SKITTER. Rotary power for drilling will be supplied by a Curvo-Synchronous motor. SKITTER is to support this system through a hexagonal shaped structure which will contain the drill motor and the power supply. A micro-coring drill will be used to remove a preliminary sample 5 mm in diameter and 20 mm long from the side of the core. This whole system is to be controlled from earth. This is carried out by a continuously monitoring PLC onboard the drill rig. A touch screen control console allows the operator on earth to monitor the progress of the operation and intervene if necessary.

  16. Hawaii Geothermal Project. Phase II: final report on well HGP-A extension to Contract E(04-3)-1093

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shupe, J.W.

    1976-07-01

    Drilling was completed on HGP-A to a depth of 6445 feet on April 27, 1976. A final core was taken; a series of logging runs performed, both with Gearhart-Owen equipment and with the Kuster temperature gauge; and the drill stem was withdrawn and laid down on the side adjacent to the rig - as a safety measure against possible volcanic tremors. A maximum temperature to date of 288/sup 0/C (550/sup 0/F) was recorded on May 13 at 4500 feet. The weighted temperature probe would penetrate no deeper into the drilling mud, which apparently is stiffening. The temperature depth relationship developedmore » in HGP-A is illustrated.« less

  17. The toll of toxics: investigating environmental contaminants

    USGS Publications Warehouse

    Sparling, Donald W.; Rattner, Barnett A.; Barclay, John S.

    2010-01-01

    On Earth Day of this year, the British Petroleum-operated Deepwater Horizon oil drilling rig exploded in the Gulf of Mexico, 41 miles off the Louisiana coast. The blast killed 11 workers, injured 17, launched a massive oil spill, and triggered an environmental catastrophe—the full impact of which may not be realized for years.

  18. 46 CFR 30.01-5 - Application of regulations-TB/ALL.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) Miscellaneous vessels, such as cable, salvage, pile-driving and oil-drilling-rig vessels: (i) Grades B, C, D..., and operations requirements relating to pollution prevention for vessels that carry oil. (a) The... United States, or which transfers oil in any port or place subject to the jurisdiction of the United...

  19. An Ultrasonic Compactor for Oil and Gas Exploration

    NASA Astrophysics Data System (ADS)

    Feeney, Andrew; Sikaneta, Sakalima; Harkness, Patrick; Lucas, Margaret

    The Badger Explorer is a rig-less oil and gas exploration tool which drills into the subsea environment to collect geological data. Drill spoil is transported from the front end of the system to the rear, where the material is compacted. Motivated by the need to develop a highly efficient compaction system, an ultrasonic compactor for application with granular geological materials encountered in subsea environments is designed and fabricated as part of this study. The finite element method is used to design a compactor configuration suitable for subsea exploration, consisting of a vibrating ultrasonic horn called a resonant compactor head, which operates in a longitudinal mode at 20 kHz, driven by a Langevin piezoelectric transducer. A simplified version of the compactor is also designed, due to its ease of incorporating in a lab-based experimental rig, in order to demonstrate enhanced compaction using ultrasonics. Numerical analysis of this simplified compactor system is supported with experimental characterisation using laser Doppler vibrometry. Compaction testing is then conducted on granular geological material, showing that compaction can be enhanced through the use of an ultrasonic compactor.

  20. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Regenhardt, C.; Dean, J.; Hancock, J.

    The purpose of this study was to determine the feasibility of the multi-drain well method in tight, lenticular formations. Although directional drilling is more costly than conventional vertical drilling, this practice could triple well production. The proposed drilling plan may be more cost efficient than drilling three separate wells with less than 320-acre spacing because it would save the costs of site surveys, rig setup, purchase of the surface lease area, and gas pipeline hookups for two additional well sites. This feasibility study was conducted on the Piceance Basin area, mainly because of the availability of geological information. The resultsmore » of this study will generally apply to other regions with tight, lenticular sand, depending upon the similarity in the total percentage of sand lenses in the area and the lens dimensions and orientations. Appendix A discusses the geology of the eastern Uinta Basin in eastern Utah, and the applicability of this study to the area. Appendix B provides calculation of expected production increase due to angle of drilling. 18 refs., 30 figs., 14 tabs.« less

  1. Phase 1 drilling operations at the Magma Energy Exploratory Well (LVF 51-20)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Finger, J.T.; Jacobson, R.D.

    1990-12-01

    This report describes the Phase 1 drilling operations for the Magma Energy Exploratory Well near Mammoth Lakes, California. An important part of the Department of Energy's Magma Energy Program, this well is designed to reach an ultimate depth of 20,000 feet or a bottomhole temperature of 500{degree}C, whichever comes first. There will be four drilling phases, at least a year apart, with scientific investigations in the borehole between the drilling intervals. Phase 1 of this project resulted in a 20 inch cased hole to 2558 feet, with 185 feet of coring beyond that. This document comprises a narrative of themore » daily activities, copies of the daily mud and lithologic reports, time breakdowns of rig activities, inventories of lost circulation materials, temperature logs of the cored hole, and a strip chart mud log. 2 figs.« less

  2. Floating production platforms and their applications in the development of oil and gas fields in the South China Sea

    NASA Astrophysics Data System (ADS)

    Zhang, Dagang; Chen, Yongjun; Zhang, Tianyu

    2014-03-01

    This paper studies the current available options for floating production platforms in developing deepwater oil fields and the potential development models of future oil and gas exploration in the South China Sea. A detailed review of current deepwater platforms worldwide was performed through the examples of industry projects, and the pros and cons of each platform are discussed. Four types of platforms are currently used for the deepwater development: tension leg platform, Spar, semi-submersible platform, and the floating production system offloading. Among these, the TLP and Spar can be used for dry tree applications, and have gained popularity in recent years. The dry tree application enables the extension of the drilling application for fixed platforms into floating systems, and greatly reduces the cost and complexity of the subsea operation. Newly built wet tree semi-submersible production platforms for ultra deepwater are also getting their application, mainly due to the much needed payload for deepwater making the conversion of the old drilling semi-submersible platforms impossible. These platforms have been used in different fields around the world for different environments; each has its own advantages and disadvantages. There are many challenges with the successful use of these floating platforms. A lot of lessons have been learned and extensive experience accumulated through the many project applications. Key technologies are being reviewed for the successful use of floating platforms for field development, and potential future development needs are being discussed. Some of the technologies and experience of platform applications can be well used for the development of the South China Sea oil and gas field.

  3. United States Transportation Command 2001 Annual Command Report: Transforming Global Mobility ... and Distribution

    DTIC Science & Technology

    2001-01-01

    Annual Command Report 13 High Speed Sealift Ship increased performance efficiencies in hull designs and innovative power plants. Promising and proven...long self-propelled, floating dock, the BLUE MARLIN was designed to haul offshore oil rigs and large, heavy cargo not unlike a ship . Whereas the COLE...the disabled ship had been returned to Mississippi for repairs. Concern about terrorism, already high , became a more concerted part of USTRANSCOM

  4. 40 CFR 124.60 - Issuance and effective date and stays of NPDES permits.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 23 2012-07-01 2012-07-01 false Issuance and effective date and stays of NPDES permits. 124.60 Section 124.60 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... provisions of § 124.16(a)(1), if, for any offshore or coastal mobile exploratory drilling rig or coastal...

  5. 40 CFR 124.60 - Issuance and effective date and stays of NPDES permits.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 22 2014-07-01 2013-07-01 true Issuance and effective date and stays of NPDES permits. 124.60 Section 124.60 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... provisions of § 124.16(a)(1), if, for any offshore or coastal mobile exploratory drilling rig or coastal...

  6. 40 CFR 124.60 - Issuance and effective date and stays of NPDES permits.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 23 2013-07-01 2013-07-01 false Issuance and effective date and stays of NPDES permits. 124.60 Section 124.60 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... provisions of § 124.16(a)(1), if, for any offshore or coastal mobile exploratory drilling rig or coastal...

  7. 40 CFR 124.60 - Issuance and effective date and stays of NPDES permits.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Issuance and effective date and stays of NPDES permits. 124.60 Section 124.60 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... provisions of § 124.16(a)(1), if, for any offshore or coastal mobile exploratory drilling rig or coastal...

  8. 40 CFR 124.60 - Issuance and effective date and stays of NPDES permits.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 22 2011-07-01 2011-07-01 false Issuance and effective date and stays of NPDES permits. 124.60 Section 124.60 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... provisions of § 124.16(a)(1), if, for any offshore or coastal mobile exploratory drilling rig or coastal...

  9. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Milgram, J.; Erb, P.R.

    A study of the behavior of floating drilling vessels during blowouts included: (1) a survey of actual incidents, (2) development of a computer model of a vessel in a blowout, and (3) large-scale experiments with a floating object in a bubble plume. The results disproved the common belief that a floating vessel will sink suddenly if a subsea blowout occurs below it. Actually, the average vertical support force changes very little in a blowout; in fact, a net upward force may result if the upwelling water flow overcomes the slight loss of fluid density due to bubbles. However, the currentsmore » caused by a blowout plume can make the vessel tip or oscillate.« less

  10. DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    The worldwide semisubmersible drilling rig fleet is approaching retirement. But replacement is not an attractive option even though dayrates are reaching record highs. In 1991, Schlumberger Sedco Forex managers decided that an alternative might exist if regulators and insurers could be convinced to extend rig life expectancy through restoration. Sedco Forex chose their No. 704 semisubmersible, an 18-year North Sea veteran, to test their process. The first step was to determine what required restoration, meaning fatigue life analysis of each weld on the huge vessel. If inspected, the task would be unacceptably time-consuming and of questionable accuracy. Instead a suitemore » of computer programs modeled the stress seen by each weld, statistically estimated the sea states seen by the rig throughout its North Sea service and calibrated a beam-element model on which to run their computer simulations. The elastic stiffness of the structure and detailed stress analysis of each weld was performed with ANSYS, a commercially available finite-element analysis program. The use of computer codes to evaluate service life extension is described.« less

  11. Vertically operating flight vehicle for drilling and agricultural use

    NASA Technical Reports Server (NTRS)

    Pabst, W.

    1986-01-01

    The invention deals with an aircraft which ascends and descends vertically and which is used for recreational aircraft, as well as for drilling and in agriculture. The invention combines the floating effect of a parachute with the helicopter to develop a flight vehicle with multiple uses which go beyond those of contemporary flight vehicles. Both hub mechanisms and thrust power are implemented to achieve this goal. Four designs are described in detail.

  12. Quick-Connect, Self-Alining Latch

    NASA Technical Reports Server (NTRS)

    Burns, G. C.; Williams, E. J.

    1983-01-01

    Sturdy latch tolerates 10 degrees of angular mismatch in joining structural elements. Hexagonal passive plate nests in active plate, guided by capture plates and alinement keys and grooves. Center hole in both active and passive plates is 1 meter in diameter. Latch has possible uses a pipe joint, connector for parts of portable structures, and fitting for marine risers on offshore drilling rigs.

  13. A Systematic Case Study of the Course, Offshore Survival Systems Training, To Improve the Practice of Marine Survival Training.

    ERIC Educational Resources Information Center

    Zambon, Franco

    A major applied research project evaluated the effectiveness of a novel course, Offshore Survival Systems Training. The major course outcome that was evaluated was the increase in frequency with which offshore personnel correctly launched the covered powered survival craft on offshore drilling rigs. The evaluation methodology included the 628…

  14. A technique for the assessment of the visual impact of nearshore confined dredged materials and other built islands

    Treesearch

    Roy Mann

    1979-01-01

    Drilling rigs, confined dredged material disposal sites power and sewage treatment facilities, and other built objects on or near shorelines have often created appreciable impacts on the aesthetic perceptions of residents and recreational users. Techniques for assessing such impacts that are reviewed in this paper include viewscape analysis for large-scale shore...

  15. One Year Later: Assessing Our Progress | NOAA Gulf Spill Restoration

    Science.gov Websites

    Archive Home One Year Later: Assessing Our Progress One Year Later: Assessing Our Progress oil in the wake Restoration Area A year ago, the Deepwater Horizon drilling rig sank, starting an oil spill that became the samples, surveyed thousands of miles of shoreline for oil, posted more than 60 work plans and other data

  16. 30 CFR 250.283 - When must I revise or supplement the approved EP, DPP, or DOCD?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...); (2) Change the surface location of a well or production platform by a distance more than that specified by the Regional Supervisor; (3) Change the type of production or significantly increase the volume... revise your approved EP, DPP, or DOCD when you propose to: (1) Change the type of drilling rig (e.g...

  17. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brink, J.

    Crude oil (c. 10,700 BOPD) was produced through temporary topside facilities in the Rolf Field offshore Denmark from January 7th to September 17th, 1986. These simple, unmanned and remote controlled facilities were a low cost solution to a problem caused by delays of the permanent topside facilities. Project execution time was two months from start of conceptual design until start-up of oil production. Installation works were performed from a jack-up drilling rig - in part simultaneously with drilling operations. Materials and equipment installed were obtained with very short delivery times. The facilties which were certified by a Certification Society andmore » approved by the Danish Authoritites included all necessary safety features. Total costs for the facilities amounted to c. 1 million US$ (excl. rig time for installation). Due to simplicity high reliability of the production system was obtained. Availability of the facilities for the entire period from start-up was 99.6 percent. The facilities were manned 3.2 percent of the total operating time mainly due to wireline work for reservoir monitoring. It is considered that the experience with the concept applied for the early production from the Rolf Field could form the basis for more simple and cost effective topside facilities for minor offshore fields.« less

  18. 30 CFR 250.428 - What must I do in certain cementing and casing situations?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... point. (h) Need to use less than required cement for the surface casing during floating drilling... permafrost zone uncemented Fill the annulus with a liquid that has a freezing point below the minimum...

  19. Comparison of heat generation during implant drilling using stainless steel and ceramic drills.

    PubMed

    Sumer, Mahmut; Misir, A Ferhat; Telcioglu, N Tuba; Guler, Ahmet U; Yenisey, Murat

    2011-05-01

    The purpose of this study was to compare the heat generated from implant drilling using stainless steel and ceramic drills. A total of 40 fresh bovine femoral cortical bone samples were used in this study. A constant drill load of 2.0 kg was applied throughout the drilling procedures via a drilling rig at a speed of 1,500 rpm. Two different implant drill types (stainless steel and ceramic) were evaluated. Heat was measured with type K thermocouple from 3 different depths. Data were subjected to the independent-sample t test and Pearson correlation analysis. The α level was set a priori at 0.05. The mean maximum temperatures at the depths of 3 mm, 6 mm, and 9 mm with the stainless steel drill were 32.15°C, 35.94°C, and 37.05°C, respectively, and those with the ceramic drill were 34.49°C, 36.73°C, and 36.52°C, respectively. A statistically significant difference was found at the depth of 3 mm (P = .014) whereas there was no significant difference at the depths of 6 and 9 mm (P > .05) between stainless steel and ceramic drills. Within the limitations of the study, although more heat was generated in the superficial part of the drilling cavity with the ceramic drill, heat modifications seemed not to be correlated with the drill type, whether stainless steel or ceramic, in the deep aspect of the cavity. Further clinical studies are required to determine the effect of drill type on heat generation. Copyright © 2011 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  20. An Anthropologist Who Studies Music and Poetics in a Rain Forest in Papua New Guinea Worries about the Future of the Natives There.

    ERIC Educational Resources Information Center

    Mangan, Katherine S.

    1991-01-01

    Living with the Kaluli of Papua New Guinea and completing his anthropology dissertation, Steven Feld saw ceremonial life begin to die and the sounds of helicopters and drill rigs compete with birds and waterfalls. Feld's sophisticated recordings preserve some ways in which the people act and blend with their environment. (MSE)

  1. 78 FR 38031 - Application for Final Commitment for a Long-Term Loan or Financial Guarantee in Excess of $100...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-25

    ... Petroquimica Basica. Description of Items Being Exported: Drilling rigs, platform rentals, compressors, oil... U.S. goods and services from U.S. small businesses to be used in its oil and gas projects. Brief non...'s on- and off-shore oil and gas exploration and production areas. To the extent that Ex-Im Bank is...

  2. 75 FR 49883 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-16

    ... incident involving a deepwater oil drilling platform occurred approximately 50 miles (80.5 km) southeast of Venice, Louisiana. An explosion and subsequent fire damaged the Deepwater Horizon MC252 oil rig, which... the 12:01 a.m., local time, July 24, 2010, closure date. Because of the Deepwater Horizon MC252 oil...

  3. Performance Analysis of Retrofitted Tribo-Corrosion Test Rig for Monitoring In Situ Oil Conditions.

    PubMed

    Siddaiah, Arpith; Khan, Zulfiqar Ahmad; Ramachandran, Rahul; Menezes, Pradeep L

    2017-09-28

    Oils and lubricants, once extracted after use from a mechanical system, can hardly be reused, and should be refurbished or replaced in most applications. New methods of in situ oil and lubricant efficiency monitoring systems have been introduced for a wide variety of mechanical systems, such as automobiles, aerospace aircrafts, ships, offshore wind turbines, and deep sea oil drilling rigs. These methods utilize electronic sensors to monitor the "byproduct effects" in a mechanical system that are not indicative of the actual remaining lifecycle and reliability of the oils. A reliable oil monitoring system should be able to monitor the wear rate and the corrosion rate of the tribo-pairs due to the inclusion of contaminants. The current study addresses this technological gap, and presents a novel design of a tribo-corrosion test rig for oils used in a dynamic system. A pin-on-disk tribometer test rig retrofitted with a three electrode-potentiostat corrosion monitoring system was used to analyze the corrosion and wear rate of a steel tribo-pair in industrial grade transmission oil. The effectiveness of the retrofitted test rig was analyzed by introducing various concentrations of contaminants in an oil medium that usually leads to a corrosive working environment. The results indicate that the retrofitted test rig can effectively monitor the in situ tribological performance of the oil in a controlled dynamic corrosive environment. It is a useful method to understand the wear-corrosion synergies for further experimental work, and to develop accurate predictive lifecycle assessment and prognostic models. The application of this system is expected to have economic benefits and help reduce the ecological oil waste footprint.

  4. Performance Analysis of Retrofitted Tribo-Corrosion Test Rig for Monitoring In Situ Oil Conditions

    PubMed Central

    Ramachandran, Rahul; Menezes, Pradeep L.

    2017-01-01

    Oils and lubricants, once extracted after use from a mechanical system, can hardly be reused, and should be refurbished or replaced in most applications. New methods of in situ oil and lubricant efficiency monitoring systems have been introduced for a wide variety of mechanical systems, such as automobiles, aerospace aircrafts, ships, offshore wind turbines, and deep sea oil drilling rigs. These methods utilize electronic sensors to monitor the “byproduct effects” in a mechanical system that are not indicative of the actual remaining lifecycle and reliability of the oils. A reliable oil monitoring system should be able to monitor the wear rate and the corrosion rate of the tribo-pairs due to the inclusion of contaminants. The current study addresses this technological gap, and presents a novel design of a tribo-corrosion test rig for oils used in a dynamic system. A pin-on-disk tribometer test rig retrofitted with a three electrode-potentiostat corrosion monitoring system was used to analyze the corrosion and wear rate of a steel tribo-pair in industrial grade transmission oil. The effectiveness of the retrofitted test rig was analyzed by introducing various concentrations of contaminants in an oil medium that usually leads to a corrosive working environment. The results indicate that the retrofitted test rig can effectively monitor the in situ tribological performance of the oil in a controlled dynamic corrosive environment. It is a useful method to understand the wear–corrosion synergies for further experimental work, and to develop accurate predictive lifecycle assessment and prognostic models. The application of this system is expected to have economic benefits and help reduce the ecological oil waste footprint. PMID:28956819

  5. Ocean Drilling Program investigates hydrate ridge

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    There are several ways to get onboard the JOIDES Resolution, a 143-meter long converted oil tanker that is a floating laboratory, and the pride of the Ocean Drilling Program (ODP). You can meet up with it at a port of call, or set down on its helicopter pad. Or you can hitch a ride by overnight supply boat, and then get lifted by crane to the Resolution by clinging to the outside of a rope transfer basket as this reporter did.

  6. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gavignet, A.A.; Wick, C.J.

    In current practice, pressure drops in the mud circulating system and the settling velocity of cuttings are calculated with simple rheological models and simple equations. Wellsite computers now allow more sophistication in drilling computations. In this paper, experimental results on the settling velocity of spheres in drilling fluids are reported, along with rheograms done over a wide range of shear rates. The flow curves are fitted to polynomials and general methods are developed to predict friction losses and settling velocities as functions of the polynomial coefficients. These methods were incorporated in a software package that can handle any rig configurationmore » system, including riser booster. Graphic displays show the effect of each parameter on the performance of the circulating system.« less

  7. [What do we know about chemical hazards in offshore work?].

    PubMed

    Moen, Bente E; Steinsvåg, Kjersti; Bråveit, Magne

    2004-10-21

    Norway has been an oil-producing nation for more than thirty years and a large number of Norwegians have been or are working on oil rigs. There are several chemical substances present on the oil platforms, and these factors may influence workers' health. The international literature on offshore chemical exposure and health is summarised. The most important groups of chemical substances used on oil rigs are described: crude oil, production chemicals, asbestos and drilling chemicals. Different types of exposure during maintenance work are described as well. Very few exposure data are published. Acute, irritative health effects from chemical exposure are described, as well as chronic health effects like skin disorders and cancer. These workers seem to have a higher risk, that may be related to benzene exposure, of developing acute myelogenous leukemia. Physicians who are treating patients working in the oil industry are advised to be aware of possible adverse health effects from the work environment on the rigs. Further exposure studies and research in this area are highly recommended, as the literature is scarce.

  8. Hercules 265 rapid response: Immediate ecosystem impacts of a natural gas blowout incident

    NASA Astrophysics Data System (ADS)

    Weber, Sarah C.; Peterson, Leigha; Battles, Jessica J.; Roberts, Brian J.; Peterson, Richard N.; Hollander, David J.; Chanton, Jeffrey P.; Joye, Samantha B.; Montoya, Joseph P.

    2016-07-01

    In late July 2013, the Hercules 265 drilling rig in the Northern Gulf of Mexico experienced a catastrophic loss of control. Large quantities of natural gas spewed into the environment for 2 days before the well self-sealed through down-hole collapse below the seafloor. Ecosystem Impacts of Oil and Gas Inputs to the Gulf (ECOGIG) and collaborating Gulf of Mexico Research Initiative (GoMRI) consortia mounted a rapid response cruise to characterize the waters around the Hercules 265 rig, beginning just 4 days after the blowout. Our analysis showed an immediate microbial response to the elevated concentrations of methane in the water column, as evidenced by the drawdown of oxygen to hypoxic conditions, the incorporation of methane-derived carbon into particles, and measurable rates of methane-assimilation and nitrogen-fixation. Additionally, radium isotope measurements allowed us to constrain the timescale of bottom water exposure to the influence of the rig. A second sampling by the Center for Integrated Modeling and Analysis of Gulf Ecosystems (C-IMAGE) consortium indicated that the ecosystem had returned to near pre-blowout conditions within one month.

  9. Using Real-time Event Tracking Sensitivity Analysis to Overcome Sensor Measurement Uncertainties of Geo-Information Management in Drilling Disasters

    NASA Astrophysics Data System (ADS)

    Tavakoli, S.; Poslad, S.; Fruhwirth, R.; Winter, M.

    2012-04-01

    This paper introduces an application of a novel EventTracker platform for instantaneous Sensitivity Analysis (SA) of large scale real-time geo-information. Earth disaster management systems demand high quality information to aid a quick and timely response to their evolving environments. The idea behind the proposed EventTracker platform is the assumption that modern information management systems are able to capture data in real-time and have the technological flexibility to adjust their services to work with specific sources of data/information. However, to assure this adaptation in real time, the online data should be collected, interpreted, and translated into corrective actions in a concise and timely manner. This can hardly be handled by existing sensitivity analysis methods because they rely on historical data and lazy processing algorithms. In event-driven systems, the effect of system inputs on its state is of value, as events could cause this state to change. This 'event triggering' situation underpins the logic of the proposed approach. Event tracking sensitivity analysis method describes the system variables and states as a collection of events. The higher the occurrence of an input variable during the trigger of event, the greater its potential impact will be on the final analysis of the system state. Experiments were designed to compare the proposed event tracking sensitivity analysis with existing Entropy-based sensitivity analysis methods. The results have shown a 10% improvement in a computational efficiency with no compromise for accuracy. It has also shown that the computational time to perform the sensitivity analysis is 0.5% of the time required compared to using the Entropy-based method. The proposed method has been applied to real world data in the context of preventing emerging crises at drilling rigs. One of the major purposes of such rigs is to drill boreholes to explore oil or gas reservoirs with the final scope of recovering the content of such reservoirs; both in onshore regions as well as in offshore regions. Drilling a well is always guided by technical, economic and security constraints to prevent crew, equipment and environment from injury, damage and pollution. Although risk assessment and local practice provides a high degree of security, uncertainty is given by the behaviour of the formation which may cause crucial situations at the rig. To overcome such uncertainties real-time sensor measurements form a base to predict and thus prevent such crises, the proposed method supports the identification of the data necessary for that.

  10. Purpose-built PDC bit successfully drills 7-in liner equipment and formation: An integrated solution

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Puennel, J.G.A.; Huppertz, A.; Huizing, J.

    1996-12-31

    Historically, drilling out the 7-in, liner equipment has been a time consuming operation with a limited success ratio. The success of the operation is highly dependent on the type of drill bit employed. Tungsten carbide mills and mill tooth rock bits required from 7.5 to 11.5 hours respectively to drill the pack-off bushings, landing collar, shoe track and shoe. Rates of penetration dropped dramatically when drilling the float equipment. While conventional PDC bits have drilled the liner equipment successfully (averaging 9.7 hours), severe bit damage invariably prevented them from continuing to drill the formation at cost-effective penetration rates. This papermore » describes the integrated development and application of an IADC M433 Class PDC bit, which was designed specifically to drill out the 7-in. liner equipment and continue drilling the formation at satisfactory penetration rates. The development was the result of a joint investigation There the operator and bit/liner manufacturers shared their expertise in solving a drilling problem, The heavy-set bit was developed following drill-off tests conducted to investigate the drillability of the 7-in. liner equipment. Key features of the new bit and its application onshore The Netherlands will be presented and analyzed.« less

  11. Catchment science on a shoestring -- Luddites, labor, and a little high-tech

    NASA Astrophysics Data System (ADS)

    Shanley, J. B.; Denner, J. C.; Clark, S. F.; Chalmers, A.; Smith, T. E.; Sebestyen, S. D.; McGlynn, B.; Hjerdt, K. N.; McDonnell, J. J.

    2004-05-01

    At Sleepers River Research Watershed in northeastern Vermont, the USGS has operated a long-term global change hydrology site with a limited equipment budget for more than 12 years. Most of the research takes place at a remote 41-ha catchment without A/C power, 15-minutes walk from the nearest road. The overall approach of the project is the combined use of hydrologic, chemical, and isotopic measurements to understand hydrologic flow paths and biogeochemical evolution of waters. Measurements and water samples are taken at various nodes in the system. Some of our cost-effective ways to conduct these measurements include: (1) a horse to pull a drill rig for installing wells in the catchment; (2) small stream V-notch weirs fashioned from plywood and plexiglas (12 years and going strong); (3) a knocking pole penetrometer to determine soil depths and infer soil properties; (4) solar panels to charge batteries; (5) frost depth measurements using methylene blue solution in flexible tubing suspended within PVC housing (6) two versions of snowmelt lysimeters constructed of PVC pipe and plexiglas (both silicone caulk and duct tape were used on the latter); (7) arrays of washing machine peepers, which are small inexpensive on/off float sensors mounted at land surface to determine surface saturation (recorded with a datalogger). These creative low-cost solutions to "getting the job done" have helped to keep this long-term site operating during budget shortfalls.

  12. The threats from oil spills: now, then, and in the future.

    PubMed

    Jernelöv, Arne

    2010-01-01

    The ongoing oil spill from the blown-out well by the name of Macondo, drilled by the ill-fated rig Deepwater Horizon, has many features in common with another blowout in the Mexican Gulf that happened three decades ago. Then the oil gushed out from the Ixtoc I well drilled by the Sedco 135-F semi-submersible rig. In the years between these catastrophes, the source and nature of oil spills have undergone large changes. Huge spills from tankers that ran aground or collided used to be what caught the headlines and caused large ecological damage. The number and size of such accidental spills have decreased significantly. Instead, spills from ageing, ill-maintained or sabotaged pipelines have increased, and places like Arctic Russia, the Niger Delta, and the northwestern Amazon have become sites of reoccurring oil pollution. As for blowouts, there is no clear trend with regard to the number of incidences or amounts of spilled oil, but deepwater blowouts are much harder to cap and thus tend to go on longer and result in the release of larger quantities of oil. Also, oil exploration and extraction is moving into ever-deeper water and into stormier and icier seas, increasing potential risks. The risk for reoccurring spills like the two huge Mexican Gulf ones is eminent and must be reduced.

  13. Geologic and operational summary, COST No. G-2 well, Georges Bank area, North Atlantic OCS

    USGS Publications Warehouse

    Amato, Roger V.; Simonis, Edvardas K.

    1980-01-01

    The Continental Offshore Stratigraphic Test (COST) No. G-2 well is the second deep well to be drilled in the Georges Bank Basin and the third in a series of COST wells on the Atlantic Outer Continental Shelf (OCS). The G-2 was drilled by Ocean Production Company, acting as the operator for 19 participating companies between January 6 and August 30, 1977. The semisubmersible rig Ocean Victory was used to drill the well to a depth of 21,874 feet at a location 132 statute miles east-southeast of Nantucket Island in 272 feet of water. An earlier deep Stratigraphic test, the COST No. G-l well, was drilled 42 statute miles west of the G-2 well, to a depth of 16,071 feet in 1976 (fig. 1). Geological and engineering data obtained from the well were used by companies and the U.S. Geological Survey (USGS) for evaluating the petroleum potential and possible drilling problems in the U.S. North Atlantic OCS area in preparation for lease sale 42 held on December 18, 1979. The Stratigraphic test was intentionally drilled away from any potential petroleum-bearing feature, but in a block bordering several tracts that were included in the sale area.

  14. Investigation of prospects for forecasting non-linear time series by example of drilling oil and gas wells

    NASA Astrophysics Data System (ADS)

    Vlasenko, A. V.; Sizonenko, A. B.; Zhdanov, A. A.

    2018-05-01

    Discrete time series or mappings are proposed for describing the dynamics of a nonlinear system. The article considers the problems of forecasting the dynamics of the system from the time series generated by it. In particular, the commercial rate of drilling oil and gas wells can be considered as a series where each next value depends on the previous one. The main parameter here is the technical drilling speed. With the aim of eliminating the measurement error and presenting the commercial speed of the object to the current with a good accuracy, future or any of the elapsed time points, the use of the Kalman filter is suggested. For the transition from a deterministic model to a probabilistic one, the use of ensemble modeling is suggested. Ensemble systems can provide a wide range of visual output, which helps the user to evaluate the measure of confidence in the model. In particular, the availability of information on the estimated calendar duration of the construction of oil and gas wells will allow drilling companies to optimize production planning by rationalizing the approach to loading drilling rigs, which ultimately leads to maximization of profit and an increase of their competitiveness.

  15. How Pecten Brazil drilled the Amazon basin

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bleakley, W.B.

    1983-09-01

    Pecten Brazil overcame numerous obstacles to drill two exploratory wells in the Amazon Basin last year. These included: The threat of low water in normally navigable rivers. Dense jungle growth at both locations. Lack of suitable roads for heavy hauling. Inconvenient distances from supply points. An unusual basalt formation responsible for unique drilling problems. Hundreds of helicopter lifts to move drilling rigs, supplies, and personnel. Pecten contracted with Petrobras, the Brazilian national oil company, to evaluate three blocks in the Amazon jungle, each about 68 miles (110 km) on a side, through seismic study and ultimate drilling. Planning for themore » drilling phase got started on March 17, 1981 with December 1 targeted as spud date for the first well. Actual spud date was November 25, 5 days ahead of schedule, in spite of all obstacles. Pecten has a mid-Amazonas block now under seismic investigation for possible exploratory drilling. Logistics problems in this one provide new difficulties, as the area is extremely wet. Most work is carried on by boat. The company is also looking offshore Bahia, testing the possible extension of the Renconcavo basin. Two wells have already provided good shows of a high pour point oil, with flow rates from 400 to 1,000 b/d. Another area of interest to Pecten is offshore Rio Grande do Norte.« less

  16. Summary: High Temperature Downhole Motor

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Raymond, David W.

    2017-10-01

    Directional drilling can be used to enable multi-lateral completions from a single well pad to improve well productivity and decrease environmental impact. Downhole rotation is typically developed with a motor in the Bottom Hole Assembly (BHA) that develops drilling power (speed and torque) necessary to drive rock reduction mechanisms (i.e., the bit) apart from the rotation developed by the surface rig. Historically, wellbore deviation has been introduced by a “bent-sub,” located in the BHA, that introduces a small angular deviation, typically less than 3 degrees, to allow the bit to drill off-axis with orientation of the BHA controlled at themore » surface. The development of a high temperature downhole motor would allow reliable use of bent subs for geothermal directional drilling. Sandia National Laboratories is pursuing the development of a high temperature motor that will operate on either drilling fluid (water-based mud) or compressed air to enable drilling high temperature, high strength, fractured rock. The project consists of designing a power section based upon geothermal drilling requirements; modeling and analysis of potential solutions; and design, development and testing of prototype hardware to validate the concept. Drilling costs contribute substantially to geothermal electricity production costs. The present development will result in more reliable access to deep, hot geothermal resources and allow preferential wellbore trajectories to be achieved. This will enable development of geothermal wells with multi-lateral completions resulting in improved geothermal resource recovery, decreased environmental impact and enhanced well construction economics.« less

  17. International oil and gas exploration and development: 1991

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1993-12-01

    This report starts where the previous quarterly publication ended. This first publication of a new annual series contains most of the same data as the quarterly report, plus some new material, through 1991. It also presents historical data covering a longer period of time than the previous quarterly report. Country-level data on oil reserves, oil production, active drilling rigs, seismic crews, wells drilled, oil reserve additions, and oil reserve-to-production rations (R/P ratios) are listed for about 85 countries, where available, from 1970 through 1991. World and regional summaries are given in both tabular and graphical form. The most popular tablemore » in the previous quarterly report, a listing of new discoveries, continues in this annual report as Appendix A.« less

  18. Exploration and Production of Hydrocarbon Resources in Coastal Alabama and Mississippi.

    DTIC Science & Technology

    1984-11-01

    and R.L. Bluntzer. 984. Laud Subsidence Near Oil and Gas Fields , Houston, Texas. Ground Water 22(4):450-459. Holzworth, G.C. 1972. Mixing Heights, Wind... field is abandoned., The operation of drilling rigs, offshore production facilities, and onshore gas and oil cleaning and processing facilities would...a pipeline releasing natural gas containing hydrogen sulfide could endanger human health and be harmful .-. to plants and animals near the point of

  19. Vega is first offshore development for Montedison

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1985-10-01

    Montedison's Vega field, 15 miles off the southern tip of Sicily, has recoverable oil reserves of 400 million bbl. This is Montedison's first offshore development venture, although the operator has considerable onshore experience. It will be followed by a second field, the smaller Mila floating production system, also off Sicily. One platform will be placed on a template installed in 1983 with up to 18 pre-drilled wells in water depths of 480 ft. The field may hold up to 1 billion bbl of 16/sup 0/ crude, but geology is complex and heavily fractured. The template has 30 available drilling slots,more » and water injection is being considered. The Vega discovery well was drilled in 1980, with 5000 b/d tested from 1000-ft oil column in Strep-penosa shales. Subsequently five wells were drilled by the Glomar Biscay I semi. These wells were drilled to a depth of just over 8000 ft with a total deviation of 60/sup 0/. The template is the first in the Mediterranean.« less

  20. DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    New fields are being added even while recent finds are brought on using floating production systems and gas pipelines. Intensive workover/redrilling continues in older onshore provinces. The paper discusses exploration, development, drilling and production in China, Indonesia, India, Malaysia, Thailand, Viet Nam, Pakistan, Myanmar, Brunei, and the Philippines, Cambodia, Bangladesh, Japan, Mongolia, and Taiwan are briefly mentioned.

  1. Quantitative Risk Assessment (QRA) for an Underground Blowout Scenario in the Gulf of Mexico (GoM) Well

    NASA Astrophysics Data System (ADS)

    Tyagi, M.; Zulqarnain, M.

    2017-12-01

    Offshore oil and gas exploration and production operations, involve the use of some of the cutting edge and challenging technologies of the modern time. These technological complex operations involves the risk of major accidents as well, which have been demonstrated by disasters such as the explosion and fire on the UK production platform piper alpha, the Canadian semi-submersible drilling rig Ocean Ranger and the explosion and capsizing of Deepwater horizon rig in the Gulf of Mexico. By conducting Quantitative Risk Assessment (QRA), safety of various operations as well as their associated risks and significance during the entire life phase of an offshore project can be quantitatively estimated. In an underground blowout, the uncontrolled formation fluids from higher pressure formation may charge up shallower overlying low pressure formations or may migrate to sea floor. Consequences of such underground blowouts range from no visible damage at the surface to the complete loss of well, loss of drilling rig, seafloor subsidence or hydrocarbons discharged to the environment. These blowouts might go unnoticed until the over pressured sands, which are the result of charging from higher pressure reservoir due to an underground blowout. Further, engineering formulas used to estimate the fault permeability and thickness are very simple in nature and may add to uncertainty in the estimated parameters. In this study the potential of a deepwater underground blowout are assessed during drilling life phase of a well in Popeye-Genesis field reservoir in the Gulf of Mexico to estimate the time taken to charge a shallower zone to its leak-off test (LOT) value. Parametric simulation results for selected field case show that for relatively high permeability (k = 40mD) fault connecting a deep over-pressured zone to a shallower low-pressure zone of similar reservoir volumes, the time to recharge the shallower zone up to its threshold LOT value is about 135 years. If the ratio of the reservoir volumes for shallower to deeper zone is about 0.1, the recharging time significantly decreased to 24 years. Also, the hydrocarbons might possibly migrate through casing-wellbore annulus due to delamination fractures between cement interfaces with rock/casing and any other micro annulus gap not isolated by cement.

  2. Dead Sea deep cores: A window into past climate and seismicity

    NASA Astrophysics Data System (ADS)

    Stein, Mordechai; Ben-Avraham, Zvi; Goldstein, Steven L.

    2011-12-01

    The area surrounding the Dead Sea was the locus of humankind's migration out of Africa and thus has been the home of peoples since the Stone Age. For this reason, understanding the climate and tectonic history of the region provides valuable insight into archaeology and studies of human history and helps to gain a better picture of future climate and tectonic scenarios. The deposits at the bottom of the Dead Sea are a geological archive of the environmental conditions (e.g., rains, floods, dust storms, droughts) during ice ages and warm ages, as well as of seismic activity in this key region. An International Continental Scientific Drilling Program (ICDP) deep drilling project was performed in the Dead Sea between November 2010 and March 2011. The project was funded by the ICDP and agencies in Israel, Germany, Japan, Norway, Switzerland, and the United States. Drilling was conducted using the new Large Lake Drilling Facility (Figure 1), a barge with a drilling rig run by DOSECC, Inc. (Drilling, Observation and Sampling of the Earth's Continental Crust), a nonprofit corporation dedicated to advancing scientific drilling worldwide. The main purpose of the project was to recover a long, continuous core to provide a high resolution record of the paleoclimate, paleoenvironment, paleoseismicity, and paleomagnetism of the Dead Sea Basin. With this, scientists are beginning to piece together a record of the climate and seismic history of the Middle East during the past several hundred thousand years in millennial to decadal to annual time resolution.

  3. Final report on evaluation of cyclocraft support of oil and gas operations in wetland areas

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Eggington, W.J.; Stevens, P.M.; John, C.J.

    1994-10-01

    The cyclocraft is a proven hybrid aircraft, capable of VTOL, lifting heavy and bulky loads, highly controllable, having high safety characteristics and low operating costs. Mission Research Corporation (MRC), under Department of Energy sponsorship, is evaluating the potential use of cyclocraft in the transport of drill rigs, mud, pipes and other materials and equipment, in a cost effective and environmentally safe manner, to support oil and gas drilling, production, and transportation operations in wetland areas. Based upon the results of an earlier parametric study, a cyclocraft design, having a payload capacity of 45 tons and designated H.1 Cyclocraft, was selectedmore » for further study, including the preparation of a preliminary design and a development plan, and the determination of operating costs. This report contains all of the results derived from the program to evaluate the use of cyclocraft in the support of oil and gas drilling and production operations in wetland areas.« less

  4. The shale gas revolution from the viewpoint of a former industry insider.

    PubMed

    Bamberger, Michelle; Oswald, Robert

    2015-02-01

    This is an interview conducted with an oil and gas worker who was employed in the industry from 1993 to 2012. He requested that his name not be used. From 2008 to 2012, he drilled wells for a major operator in Bradford County, Pennsylvania. Bradford County is the center of the Marcellus shale gas boom in Northeastern Pennsylvania. In 2012, he formed a consulting business to assist clients who need information on the details of gas and oil drilling operations. In this interview, the worker describes the benefits and difficulties of the hard work involved in drilling unconventional gas wells in Pennsylvania. In particular, he outlines the safety procedures that were in place and how they sometimes failed, leading to workplace injuries. He provides a compelling view of the trade-offs between the economic opportunities of working on a rig and the dangers and stresses of working long hours under hazardous conditions. © 2015 SAGE Publications.

  5. USEPA/USGS Sample Collection Protocol for Bacterial ...

    EPA Pesticide Factsheets

    Report/SOP This Sample Collection Procedure (SCP) describes the activities and considerations for the collection of bacterial pathogens from representative surface soil samples (0-5 cm). This sampling depth can be reached without the use of a drill rig, direct-push technology, or other mechanized equipment. Analizing soil samples for biothreat agents may, for instance, define the extent of contamination or determine whether the concentrations of contaminants present a risk to public health, welfare, or the environment.

  6. Vectorization of a penalty function algorithm for well scheduling

    NASA Technical Reports Server (NTRS)

    Absar, I.

    1984-01-01

    In petroleum engineering, the oil production profiles of a reservoir can be simulated by using a finite gridded model. This profile is affected by the number and choice of wells which in turn is a result of various production limits and constraints including, for example, the economic minimum well spacing, the number of drilling rigs available and the time required to drill and complete a well. After a well is available it may be shut in because of excessive water or gas productions. In order to optimize the field performance a penalty function algorithm was developed for scheduling wells. For an example with some 343 wells and 15 different constraints, the scheduling routine vectorized for the CYBER 205 averaged 560 times faster performance than the scalar version.

  7. The Chesapeake Bay Impact Crater: An Educational Investigation for Students into the Planetary Impact Process and its Environmental Consequences

    NASA Technical Reports Server (NTRS)

    Levine, Arlene S.

    2008-01-01

    Planetary impact craters are a common surface feature of many planetary bodies, including the Earth, the Moon, Mars, Mercury, Venus, and Jupiter s moons, Ganymede and Callisto. The NASA Langley Research Center in Hampton, VA, is located about 5 km inside the outer rim of the Chesapeake Bay Impact Crater. The Chesapeake Bay Impact Crater, with a diameter of 85 km is the sixth largest impact crater on our planet. The U.S. Geological Survey (USGS), in collaboration with the NASA Langley Research Center, the Virginia Department of Environmental Quality (VDEQ), the Hampton Roads Planning District Commission (HRPDC), and the Department of Geology of the College of William and Mary (WM) drilled into and through the crater at the NASA Langley Research Center and obtained a continuous core to a depth of 2075.9 ft (632.73 meters) from the Chesapeake Bay Impact Crater. At the NASA Langley location, the granite basement depth was at 2046 ft (623.87 meters). This collaborative drilling activity provided a unique educational opportunity and ongoing educational partnership between USGS, NASA Langley and the other collaborators. NASA Langley has a decade-long, ongoing educational partnership with the Colonial Coast Council of the Girl Scouts. The core drilling and on site analysis and cataloguing of the core segments provided a unique opportunity for the Girl Scouts to learn how geologists work in the field, their tools for scientific investigation and evaluation, how they perform geological analyses of the cores in an on-site tent and learn about the formation of impact craters and the impact of impacting bodies on the sub-surface, the surface, the oceans and atmosphere of the target body. This was accomplished with a two-part activity. Girl Scout day camps and local Girl Scout troops were invited to Langley Research Center Conference Center, where more than 300 Girl Scouts, their leaders and adult personnel were given briefings by scientists and educators from the USGS, NASA, VDEQ, HRPDC and WM on the principles of geology, the formation of impact craters, the consequences of the impacting body on the atmosphere, ocean, surface and sub-surface, the geological, chemical and biological analyses of the core and the cataloguing and storage of the core segments, etc. After the briefings, the Girl Scouts visited the drilling site where they inspected the core drilling rig, examined the core samples and discussed the drilling procedures, cores and interpretation of the cores with scientists and educators from the organizations conducting the core drilling. Demonstrations at the drilling site included demonstrations of impacting objects hitting multi-colored layered mud targets at different angles of entry. The multi-colored layers of mud were instructive in mapping out the distribution of impact-ejected material around the impact crater. The presentation will include a series of photographs of the Girl Scout participating in activities at the Chesapeake Bay Impact Crater drill site, including retrieving cores from the drilling rig, inspecting the core samples and participating in the impact-crater formation demonstrations.

  8. Nonlinear quasi-static analysis of ultra-deep-water top-tension riser

    NASA Astrophysics Data System (ADS)

    Gao, Guanghai; Qiu, Xingqi; Wang, Ke; Liu, Jianjun

    2017-09-01

    In order to analyse the ultra-deep-water top-tension riser deformation in drilling conditions, a nonlinear quasi-static analysis model and equation are established. The riser in this model is regarded as a simply supported beam located in the vertical plane and is subjected to non-uniform axial and lateral forces. The model and the equation are solved by the finite element method. The effects of riser outside diameter, top tension ratio, sea surface current velocity, drag force coefficient, floating system drift distance and water depth on the riser lateral displacement are discussed. Results show that the riser lateral displacement increase with the increase in the sea surface current velocity, drag force coefficient and water depth, whereas decrease with the increase in the riser outside diameter, top tension ratio. The top tension ratio has an important influence on the riser deformation and it should be set reasonably under different circumstances. The drift of the floating system has a complicated influence on the riser deformation and it should avoid a large drift distance in the proceedings of drilling and production.

  9. Efficiency in energy production and consumption

    NASA Astrophysics Data System (ADS)

    Kellogg, Ryan Mayer

    This dissertation deals with economic efficiency in the energy industry and consists of three parts. The first examines how joint experience between pairs of firms working together in oil and gas drilling improves productivity. Part two asks whether oil producers time their drilling optimally by taking real options effects into consideration. Finally, I investigate the efficiency with which energy is consumed, asking whether extending Daylight Saving Time (DST) reduces electricity use. The chapter "Learning by Drilling: Inter-Firm Learning and Relationship Persistence in the Texas Oilpatch" examines how oil production companies and the drilling rigs they hire improve drilling productivity by learning through joint experience. I find that the joint productivity of a lead firm and its drilling contractor is enhanced significantly as they accumulate experience working together. Moreover, this result is robust to other relationship specificities and standard firm-specific learning-by-doing effects. The second chapter, "Drill Now or Drill Later: The Effect of Expected Volatility on Investment," investigates the extent to which firms' drilling behavior accords with a key prescription of real options theory: irreversible investments such as drilling should be deferred when the expected volatility of the investments' payoffs increases. I combine detailed data on oil drilling with expectations of future oil price volatility that I derive from the NYMEX futures options market. Conditioning on expected price levels, I find that oil production companies significantly reduce the number of wells they drill when expected price volatility is high. I conclude with "Daylight Time and Energy: Evidence from an Australian Experiment," co-authored with Hendrik Wolff. This chapter assesses DST's impact on electricity demand using a quasi-experiment in which parts of Australia extended DST in 2000 to facilitate the Sydney Olympics. We show that the extension did not reduce overall electricity consumption, but did cause a substantial intra-day shift in demand consistent with activity patterns that are tied to the clock rather than sunrise and sunset.

  10. Engagement of Metal Debris into Gear Mesh

    NASA Technical Reports Server (NTRS)

    handschuh, Robert F.; Krantz, Timothy L.

    2010-01-01

    A series of bench-top experiments was conducted to determine the effects of metallic debris being dragged through meshing gear teeth. A test rig that is typically used to conduct contact fatigue experiments was used for these tests. Several sizes of drill material, shim stock and pieces of gear teeth were introduced and then driven through the meshing region. The level of torque required to drive the "chip" through the gear mesh was measured. From the data gathered, chip size sufficient to jam the mechanism can be determined.

  11. Study on a Mechanical Semi-Active Heave Compensation System of Drill String for Use on Floating Drilling Platform

    PubMed Central

    Liu, Qingyou; Tang, Yang; Huang, Chongjun; Xie, Chong

    2015-01-01

    There are some disadvantages for existing heave compensation systems of drill string used for the Floating Drilling Platform (FDP), including high energy consumption, large and complex structure, and expensive manufacturing and maintenance costs. In view of the above, we present a streamlined mechanical semi-active heave compensation system (MSAHC) in this study. This system consists of active compensation part with the pinion and rack and passive compensation part. In order to evaluate system performance of the MSAHC, we establish its simulation model with AMEsim software. In the process of simulation, displacement of rotary hook and energy consumption is regarded as performance parameters of the system. And the change rule of two performance parameters are analyzed by changing these design parameters including gear radius of the pinion and rack, scale coefficient of PID, rotary hook load, heave height and heave period of the FDP, and accumulator volume. Then, based on the simulation results of the MSAHC system performance, we have selected out a best set of design parameters from them. Moreover, the feasibility of the design scheme of the MSAHC is effectively verified by comparison with the existing three heave compensation system. The result shows that the energy consumption of the MSAHC is lower than the active heave compensation system (AHC) and the semi-active heave compensation system (SAHC) when achieving a same compensation effect as well as the accumulator volume of MSAHC is half of the passive heave compensation system (PHC). Therefore, the new designed MSAHC not only ensure compensation effect but also lower energy consumption, and its structure is simplified by adopting the simple mechanical structure to decrease manufacturing cost, maintenance cost and floor space. PMID:26186620

  12. Infill and mire evolution of a typical kettle hole: young ages at great depths (Jackenmoos, Austria)

    NASA Astrophysics Data System (ADS)

    Götz, Joachim; Salcher, Bernhard

    2015-04-01

    Kettle holes are very common features in proglacial environments. Myriads of small, often circular shaped lakes are indicative of dead ice slowly melting out after the collapse of glaciers and subsequent burial of glaciofluvial sediments. Many of these lakes transformed into mires during the Postglacial and the Holocene. Still, little is known about the mechanisms leading to mire formation in such environments. We aim to analyse the shape and the postglacial history of infilling and peat accumulation of a typical dead ice kettle using 2D resistivity surveying, core-drilling, 14C dating and palynologic analyses. The kettle hole mire is located within a small kame delta deposit just south of the LGM extend of the Salzach Piedmont glacier (Austria/Germany). Today, the mire is a spot of exceptional high biodiversity and under protection. Sediment core samples extracted in the deepest (c. 10-14 m) and central part of the kettle directly overly lacustrine fine sediments and yielded young ages covering the subatlantic period only. Young ages are in agreement with palynologic results comprising e.g. pollen of secale (rye) and juglans (walnut). However, these deposits are situated beneath a massive water body (10 m), only covered by a thin floating mat. A second, more distally situated drill core indicates the thinning of this water body at the expense of peat deposits covering the Late Glacial to Middle Holocene. Multiple 2D resistivity data support drilling information and enabled us to reconstruct the shape of the basin. The transition from lacustrine sediments to the water body above is characterised by a sharp increase in resistivity. Furthermore, the resistivity pattern within the entire kettle indicates an increase towards the centre, most probably as a result of the changing nutrient content. The postglacial evolution of the mire is in agreement with the concept of "floating mat terrestrialisation", representing a horizontal growth of the floating mat from the edges toward the lake centre. This concept further includes the deposition of strongly hydrated and loose debris peat formations under the floating mat. The process leads to decreasing basal ages from the edge towards the centre and therefore well explains the age distribution in the studied kettle hole.

  13. VSAT: opening new horizons to oil and gas explorations

    NASA Astrophysics Data System (ADS)

    Al-Dhamen, Muhammad I.

    2002-08-01

    Whether exploring in the Empty Quarter, drilling offshore in the Gulf of Mexico, or monitoring gas pipelines or oil wells in the deserts, communications is a key element to the success of oil and gas operations. Secure, efficient communications is required between remote, isolated locations and head offices to report on work status, dispatch supplies and repairs, report on-site emergencies, transfer geophysical surveys and real-time drilling data. Drilling and exploration firms have traditionally used land-based terrestrial networks that rely on radio transmissions for voice and data communications to offshore platforms and remote deep desert drilling rigs. But these systems are inefficient and have proven inflexible with today's drilling and exploration communications demands, which include high-speed data access, telephone and video conferencing. In response, numerous oil and gas exploration entities working in deep waters and remote deep deserts have all tapped into what is an ideal solution for these needs: Very Small Aperture Terminal Systems (VSAT) for broadband access services. This led to the use of Satellite Communication Systems for a wide range of applications that were difficult to achieve in the past, such as real-time applications transmission of drilling data and seismic information. This paper provides a thorough analysis of opportunities for satellite technology solutions in support of oil and gas operations. Technologies, architecture, service, networking and application developments are discussed based upon real field experience. More specifically, the report addresses: VSAT Opportunities for the Oil and Gas Operations, Corporate Satellite Business Model Findings, Satellite Market Forecasts

  14. Association of ice and river channel morphology determined using ground-penetrationg radar in the Kuparuk River, Alaska

    USGS Publications Warehouse

    Best, Heather; McNamara, J.P.; Liberty, Lee M.

    2005-01-01

    We collected ground-penetrating radar data at 10 sites along the Kuparuk River and its main tributary, the Toolik River, to detect unfrozen water beneath river ice. We used 250 MHz and 500 MHz antennas to image both the ice-water interface and the river channel in late April 2001, when daily high temperatures were consistently freezing and river ice had attained its maximum seasonal thickness. The presence of water below the river ice appears as a strong, horizontal reflection observed in the radar data and is confirmed by drill hole data. A downstream transition occurs from ice that is frozen to the bed, called bedfast ice, to ice that is floating on unfrozen water, called floating ice. This transition in ice type corresponds to a downstream change in channel size that was detected in previously conducted hydraulic geometry surveys of the Kuparuk River. We propose a conceptual model wherein the downstream transition from bedfast ice to floating ice is responsible for an observed step change in channel size due to enhanced bank erosion in large channels by floating ice.

  15. Estimating gas kick arrival to better manage surface flow

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Merryman, J.

    1997-06-01

    There are many quality, expertly designed programs for teaching rig crews prevention and control of well kicks. Much time and money has been spent training rig crews for procedures and methods to avoid kicks, and what to do if you have one, even though all accepted practices were followed. One false/bad result from this training is that individuals have come away from this training believing that once a gas kick is circulated through the choke, all problems are simple and manageable, i.e., the danger has passed. What you do with gas once it is downstream of the choke has notmore » been given enough emphasis in some training programs, including the one the author is involved in. This article, prepared from experiences on actual rig locations in Wyoming and Argentina, and developed for well control training in Parker Drilling Co.`s Training Center, Odessa, Texas, describes a method for estimating the time, in pump strokes and/or minutes, for a gas kick to reach surface. Reducing pump strokes (circulating flowrate) and knowing available time to gas kick arrival allows the crew to prepare surface handling equipment. Common misconceptions about gas handling and potential resulting problems are noted. An example calculation describes the calculation procedure.« less

  16. Field Testing of Environmentally Friendly Drilling System

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    David Burnett

    2009-05-31

    The Environmentally Friendly Drilling (EFD) program addresses new low-impact technology that reduces the footprint of drilling activities, integrates light weight drilling rigs with reduced emission engine packages, addresses on-site waste management, optimizes the systems to fit the needs of a specific development sites and provides stewardship of the environment. In addition, the program includes industry, the public, environmental organizations, and elected officials in a collaboration that addresses concerns on development of unconventional natural gas resources in environmentally sensitive areas. The EFD program provides the fundamentals to result in greater access, reasonable regulatory controls, lower development cost and reduction of themore » environmental footprint associated with operations for unconventional natural gas. Industry Sponsors have supported the program with significant financial and technical support. This final report compendium is organized into segments corresponding directly with the DOE approved scope of work for the term 2005-2009 (10 Sections). Each specific project is defined by (a) its goals, (b) its deliverable, and (c) its future direction. A web site has been established that contains all of these detailed engineering reports produced with their efforts. The goals of the project are to (1) identify critical enabling technologies for a prototype low-impact drilling system, (2) test the prototype systems in field laboratories, and (3) demonstrate the advanced technology to show how these practices would benefit the environment.« less

  17. Hydraulics calculation in drilling simulator

    NASA Astrophysics Data System (ADS)

    Malyugin, Aleksey A.; Kazunin, Dmitry V.

    2018-05-01

    The modeling of drilling hydraulics in the simulator system is discussed. This model is based on the previously developed quasi-steady model of an incompressible fluid flow. The model simulates the operation of all parts of the hydraulic drilling system. Based on the principles of creating a common hydraulic model, a set of new elements for well hydraulics was developed. It includes elements that correspond to the in-drillstring and annular space. There are elements controlling the inflow from the reservoir into the well and simulating the lift of gas along the annulus. New elements of the hydrosystem take into account the changing geometry of the well, loss in the bit, characteristics of the fluids including viscoplasticity. There is an opportunity specify the complications, the main one of which is gas, oil and water inflow. Correct work of models in cases of complications makes it possible to work out various methods for their elimination. The coefficients of the model are adjusted on the basis of incomplete experimental data provided by operators of drilling platforms. At the end of the article the results of modeling the elimination of gas inflow by a continuous method are presented. The values displayed in the simulator (drill pipe pressure, annulus pressure, input and output flow rates) are in good agreement with the experimental data. This exercise took one hour, which is less than the time on a real rig with the same configuration of equipment and well.

  18. 78 FR 27913 - Revision of Crane Regulation Standards for Mobile Offshore Drilling Units (MODUs), Offshore...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-13

    ...The Coast Guard proposes to revise regulations related to the design, certification, inspection, and testing of cranes. These regulations apply to cranes installed on Mobile Offshore Drilling Units (MODUs), Offshore Supply Vessels (OSVs), and floating Outer Continental Shelf (OCS) facilities. This revision would update industry standards incorporated by reference with more recent versions, which are used by industry and incorporated in Bureau of Safety and Environmental Enforcement regulations. Additionally, the Coast Guard proposes to revise regulations regarding certification, inspection, and testing of cranes by allowing use of additional organizations to act in lieu of Coast Guard marine inspectors.

  19. Application of Formation Testing While Drilling (GeoTap) for acquiring formation pressure data from the Azeri, Chirag and Guneshli wells which were drilled in the Khazarian-Caspian Sea of the Azerbaijan Republic

    NASA Astrophysics Data System (ADS)

    Amirov, Elnur

    2016-04-01

    A new technology to acquire wireline quality pressure tests using a Logging While Drilling approach has been successfully implemented few years ago in Azeri, Chirag and Guneshli wells which were drilled in the Khazarian-Caspian Sea of the Azerbaijan Republic. The Formation Tester While Drilling tool (GeoTap) uses a testing sequence similar to wireline tools. A single probe is extended to the borehole wall and a small pretest volume withdrawn from the formation. The resulting pressure transient is then analyzed for formation pressure, formation permeability and mobility information. Up-link and down-link capabilities have been added to achieve test control and quality feedback. An efficient downlink algorithm is used downhole to analyze the data. The parameters and pressure data are transmitted to the surface in real-time for continuous monitoring of the test. More detailed pressure data is recorded and retrieved after returning to surface. Use of a quartz gauge allows excellent accuracy. Azeri, Chirag and Guneshli fields consist of layered sand reservoirs alternation with shale sequences and detailed pressure data is acquired on a high percentage of wells in order to understand lateral and vertical continuity of different flow units. The formation tester can be utilized with the 'triple combo' Logging While Drilling string which eliminates the need to rig up wireline on many wells. Wireline formation tester runs are time consuming - particularly if high deviation or high overbalance conditions are encountered requiring pipe conveyed techniques. Non-Productive Time is high when the wireline tools are stuck and fishing operations are required. The Sperry Drilling GeoTap formation pressure tester service provides real-time formation pressure measurements. It bridges the critical gap between drilling safety and optimization, by providing early and reliable measurements of key reservoir properties, while improving reservoir understanding and completion design in real time. The GeoTap tester obtains direct pore-pressure measurements as the well is being drilled, with accuracy and precision comparable to that of wireline testers. The GeoTap service can eliminate the time, risk, and cost associated with running pipe-conveyed wireline test tools. It also measures annular and bore pressure while drilling, providing accurate, continuous, real-time hydrostatic pressure, and equivalent circulating density (ECD) information. This aids in determining and maintaining optimal mud weight, reduces formation damage, increases the rate of penetration, and increases operational safety. GeoTap benefits can be improvement of formation evaluation, real-time fluid gradients and fluid mobility (permeability/viscosity indicator), identification of fluid contact points, determination of reservoir connectivity/compartmentalization and depletion, increase safety of operation, determination of optimal mud weight and manage of ECD. We can also continuously monitor wellbore stability for assessments in order to reduce formation damage which in turns will help to increase drilling effectiveness (determine precise overbalance for maximizing ROP and continuously monitor hole-cleaning effectiveness with pressure-while-drilling, while reducing formation damage due to swab/surge). Save time and money by reducing rig down time associated with wireline testing. GeoTap Tool capable of performing more than 150 pressure tests per run and optional orientation of pressure measurement is available (top, right, bottom or left). GeoTap testing has been completed with encouraging results in many wells up to circa 3000m deep. Data has been acquired successfully both in a "Drill-Test-Drill' mode and a "Post-Drill-Test" mode. GeoTap tests have spanned wide ranges of borehole temperature, pressure, mobility as well as formation permeability and overbalance conditions. GeoTap tests in Azeri, Chirag and Guneshli wells which were drilled in the Khazarian-Caspian Sea of the Azerbaijan Republic have proved that a logging while drilling approach can be successfully employed to acquire formation pressure data in open hole (which is also very useful for fluid gradient analysis, oil water and gas oil contacts delineation/identification).

  20. Engagement of Metal Debris into a Gear Mesh

    NASA Technical Reports Server (NTRS)

    Handschuh, Robert F.; Krantz, Timothy L.

    2010-01-01

    A series of bench top experiments was conducted to determine the effects of metallic debris being dragged through meshing gear teeth. A test rig that is typically used to conduct contact fatigue experiments was used for these tests. Several sizes of drill material, shim stock, and pieces of gear teeth were introduced and then driven through the meshing region. The level of torque required to drive the "chip" through the gear mesh was measured. From the data gathered, chip size sufficient to jam the mechanism can be determined.

  1. Antiwhirl PDC bits increased penetration rates in Alberta drilling. [Polycrystalline Diamond Compact

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bobrosky, D.; Osmak, G.

    1993-07-05

    The antiwhirl PDC bits and an inhibitive mud system contributed to the quicker drilling of the time-sensitive shales. The hole washouts in the intermediate section were dramatically reduced, resulting in better intermediate casing cement jobs. Also, the use of antirotation PDC-drillable cementing plugs eliminated the need to drill out plugs and float equipment with a steel tooth bit and then trip for the PDC bit. By using an antiwhirl PDC bit, at least one trip was eliminated in the intermediate section. Offset data indicated that two to six conventional bits would have been required to drill the intermediate hole interval.more » The PDC bit was rebuildable and therefore rerunnable even after being used on five wells. In each instance, the cost of replacing chipped cutters was less than the cost of a new insert roller cone bit. The paper describes the antiwhirl bits; the development of the bits; and their application in a clastic sequence, a carbonate sequence, and the Shekilie oil field; the improvement in the rate of penetration; the selection of bottom hole assemblies; washout problems; and drill-out characteristics.« less

  2. Letter from Europe: oil business expands; drills go deeper

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jenkins, N.

    A view of the energy picture as seen from England indicates a great deal of drilling activity is taking place in the North Sea, where 65 platform rigs are producing enough oil to have reduced England's costs for imported oil. Promising new fields southeast and west of the Shetland Islands have not slowed British efforts to keep reserve levels ahead of depletion rates. The Italians are working at depths of up to 1100 meters to extend their gas fields in the Adriatic and Ionian waters. Australia is undertaking a major oil exploration effort in the west and northwest, with competingmore » efforts of several oil companies. The uranium market is complicated by environmental problems, although Canada may export some to Germany and England on a trial basis. Delegates to the London Local Energy Center Conference felt that the United Kingdom needs a total energy policy. The electricity industry opposes this view. (DCK)« less

  3. Effects of tones associated with drilling activities on bowhead whale calling rates

    PubMed Central

    Nations, Christopher S.; Thode, Aaron M.; Kauffman, Mandy E.; Conrad, Alexander S.; Norman, Robert G.; Kim, Katherine H.

    2017-01-01

    During summer 2012 Shell performed exploratory drilling at Sivulliq, a lease holding located in the autumn migration corridor of bowhead whales (Balaena mysticetus), northwest of Camden Bay in the Beaufort Sea. The drilling operation involved a number of vessels performing various activities, such as towing the drill rig, anchor handling, and drilling. Acoustic data were collected with six arrays of directional recorders (DASARs) deployed on the seafloor over ~7 weeks in Aug–Oct. Whale calls produced within 2 km of each DASAR were identified and localized using triangulation. A “tone index” was defined to quantify the presence and amplitude of tonal sounds from industrial machinery. The presence of airgun pulses originating from distant seismic operations was also quantified. For each 10-min period at each of the 40 recorders, the number of whale calls localized was matched with the “dose” of industrial sound received, and the relationship between calling rates and industrial sound was modeled using negative binomial regression. The analysis showed that with increasing tone levels, bowhead whale calling rates initially increased, peaked, and then decreased. This dual behavioral response is similar to that described for bowhead whales and airgun pulses in earlier work. Increasing call repetition rates can be a viable strategy for combating decreased detectability of signals arising from moderate increases in background noise. Meanwhile, as noise increases, the benefits of calling may decrease because information transfer becomes increasingly error-prone, and at some point calling may no longer be worth the effort. PMID:29161308

  4. Effects of tones associated with drilling activities on bowhead whale calling rates.

    PubMed

    Blackwell, Susanna B; Nations, Christopher S; Thode, Aaron M; Kauffman, Mandy E; Conrad, Alexander S; Norman, Robert G; Kim, Katherine H

    2017-01-01

    During summer 2012 Shell performed exploratory drilling at Sivulliq, a lease holding located in the autumn migration corridor of bowhead whales (Balaena mysticetus), northwest of Camden Bay in the Beaufort Sea. The drilling operation involved a number of vessels performing various activities, such as towing the drill rig, anchor handling, and drilling. Acoustic data were collected with six arrays of directional recorders (DASARs) deployed on the seafloor over ~7 weeks in Aug-Oct. Whale calls produced within 2 km of each DASAR were identified and localized using triangulation. A "tone index" was defined to quantify the presence and amplitude of tonal sounds from industrial machinery. The presence of airgun pulses originating from distant seismic operations was also quantified. For each 10-min period at each of the 40 recorders, the number of whale calls localized was matched with the "dose" of industrial sound received, and the relationship between calling rates and industrial sound was modeled using negative binomial regression. The analysis showed that with increasing tone levels, bowhead whale calling rates initially increased, peaked, and then decreased. This dual behavioral response is similar to that described for bowhead whales and airgun pulses in earlier work. Increasing call repetition rates can be a viable strategy for combating decreased detectability of signals arising from moderate increases in background noise. Meanwhile, as noise increases, the benefits of calling may decrease because information transfer becomes increasingly error-prone, and at some point calling may no longer be worth the effort.

  5. Channelized bottom melting and stability of floating ice shelves

    NASA Astrophysics Data System (ADS)

    Rignot, E.; Steffen, K.

    2008-01-01

    The floating ice shelf in front of Petermann Glacier, in northwest Greenland, experiences massive bottom melting that removes 80% of its ice before calving into the Arctic Ocean. Detailed surveys of the ice shelf reveal the presence of 1-2 km wide, 200-400 m deep, sub-ice shelf channels, aligned with the flow direction and spaced by 5 km. We attribute their formation to the bottom melting of ice from warm ocean waters underneath. Drilling at the center of one of channel, only 8 m above sea level, confirms the presence of ice-shelf melt water in the channel. These deep incisions in ice-shelf thickness imply a vulnerability to mechanical break up and climate warming of ice shelves that has not been considered previously.

  6. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Munganahalli, D.

    Sedco Forex is a drilling contractor that operates approximately 80 rigs on land and offshore worldwide. The HSE management system developed by Sedco Forex is an effort to prevent accidents and minimize losses. An integral part of the HSE management system is establishing risk profiles and thereby minimizing risk and reducing loss exposures. Risk profiles are established based on accident reports, potential accident reports and other risk identification reports (RIR) like the Du Pont STOP system. A rig could fill in as many as 30 accident reports, 30 potential accident reports and 500 STOP cards each year. Statistics are importantmore » for an HSE management system, since they are indicators of success or failure of HSE systems. It is however difficult to establish risk profiles based on statistical information, unless tools are available at the rig site to aid with the analysis. Risk profiles are then used to identify important areas in the operation that may require specific attention to minimize the loss exposure. Programs to address the loss exposure can then be identified and implemented with either a local or corporate approach. In January 1995, Sedco Forex implemented a uniform HSE Database on all the rigs worldwide. In one year companywide, the HSE database would contain information on approximately 500 accident and potential accident reports, and 10,000 STOP cards. This paper demonstrates the salient features of the database and describes how it has helped in establishing key risk profiles. It also shows a recent example of how risk profiles have been established at the corporate level and used to identify the key contributing factors to hands and finger injuries. Based on this information, a campaign was launched to minimize the frequency of occurrence and associated loss attributed to hands and fingers accidents.« less

  7. Cranes, hoists, and rigging. A safety training manual

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stinnett, L.

    The handling equipment discussed in this manual is the type that lifts, lowers, and locates (positions) heavy material - cranes and hoists. The crane/hoist family of equipment may be as small as a single pulley/rope system handling several pounds, or as large as a marine crane system that recently (1985) hoisted a 5005-metric ton oil drilling rig from a barge and placed it on the seabed of England's North Atlantic offshore oil field. An equivalent ''pick'' would be a 6-ft-high solid pine board, 360 ft long and 160 ft wide (the dimensions of a football field). The capacity of themore » cranes and hoists at Sandia National Laboratories in Albuquerque (SNLA) fits somewhere within the first 2% of the marine pick or lift described above. SNLA has several mobile cranes and more than 500 other types of cranes and hoists located in more than 100 buildings. The Department of Energy (DOE) and SNL have safety policies and directives that require the operators of heavy equipment to be trained and authorized. The Cranes, Hoists, and Rigging Safety Training Program, sponsored by the SNLA Safety Department, is an effort to fulfill those safety policy requirements. This manual will be used as a safety training aid and will be issued as a reference document for supervisors, operators, inspectors, and service personnel who use cranes or hoists during their regular duties.« less

  8. Simple, Affordable and Sustainable Borehole Observatories for Complex Monitoring Objectives

    NASA Astrophysics Data System (ADS)

    Kopf, A.; Freudenthal, T.; Ratmeyer, V.; Wefer, G.

    2014-12-01

    Seafloor drill rigs are remotely operated systems that provide a cost effective means to recover sedimentary records of the upper sub-seafloor deposits. Recent increases in their payload included downhole logging tools or autoclave coring systems. We here report on another milestone in using seafloor rigs: The development and installation of shallow borehole observatories. Three different systems have been developed for the MeBo seafloor drill, which is operated by MARUM, Univ. Bremen, Germany. A simple design, the MeBoPLUG, separates the inner borehole from the overlying ocean by using o-ring seals at the conical threads of the drill pipe. The systems are self-contained and include data loggers, batteries, thermistors and a differential pressure sensor. A second design, the so-called MeBoCORK, is more sophisticated and also hosts an acoustic modem for data transfer and, if desired, fluid sampling capability using osmotic pumps. Of these MeBoCORKs, two systems have to be distinguished: The CORK-A (A = autonomous) can be installed by the MeBo alone and monitors pressure and temperature inside and above the borehole (the latter for reference). The CORK-B (B = bottom) has a higher payload and can additionally be equipped with geochemical, biological or other physical components. Owing to its larger size, it is installed by ROV and utilises a hotstab connection in the upper portion of the drill string. Either design relies on a hostab connection from beneath which coiled tubing with a conical drop weight is lowered to couple to the formation. These tubes are fluid-saturated and either serve to transmit pore pressure signals or collect pore water in the osmo-sampler. The third design, the MeBoPUPPI (Pop-Up Pore Pressure Instrument), is similar to the MeBoCORK-A and monitors pore pressure and temperature in a self-contained manner. Instead of transferring data upon command using an acoustic modem, the MeBoPUPPI contains a pop-up telemetry with Iridium link. After a predefined period, the data unit with satellite link is released, ascends to the sea surface, and remains there for up to two weeks while sending the long-term data sets to shore. In 2012, 2 MeBoPLUGs, 1 MeBoCORK-A and 1 MeBoCORK-B were installed with MeBo in the Nankai Trough, Japan, and data were successfully downloaded from the CORKs.

  9. Use of Cutting-Edge Horizontal and Underbalanced Drilling Technologies and Subsurface Seismic Techniques to Explore, Drill and Produce Reservoired Oil and Gas from the Fractured Monterey Below 10,000 ft in the Santa Maria Basin of California

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    George Witter; Robert Knoll; William Rehm

    2005-09-29

    This project was undertaken to demonstrate that oil and gas can be drilled and produced safely and economically from a fractured Monterey reservoir in the Santa Maria Basin of California by employing horizontal wellbores and underbalanced drilling technologies. Two vertical wells were previously drilled in this area with heavy mud and conventional completions; neither was commercially productive. A new well was drilled by the project team in 2004 with the objective of accessing an extended length of oil-bearing, high-resistivity Monterey shale via a horizontal wellbore, while implementing managed-pressure drilling (MPD) techniques to avoid formation damage. Initial project meetings were conductedmore » in October 2003. The team confirmed that the demonstration well would be completed open-hole to minimize productivity impairment. Following an overview of the geologic setting and local field experience, critical aspects of the application were identified. At the pre-spud meeting in January 2004, the final well design was confirmed and the well programming/service company requirements assigned. Various design elements were reduced in scope due to significant budgetary constraints. Major alterations to the original plan included: (1) a VSP seismic survey was delayed to a later phase; (2) a new (larger) surface hole would be drilled rather than re-enter an existing well; (3) a 7-in. liner would be placed into the top of the Monterey target as quickly as possible to avoid problems with hole stability; (4) evaluation activities were reduced in scope; (5) geosteering observations for fracture access would be deduced from penetration rate, cuttings description and hydrocarbon in-flow; and (6) rather than use nitrogen, a novel air-injection MPD system was to be implemented. Drilling operations, delayed from the original schedule by capital constraints and lack of rig availability, were conducted from September 12 to November 11, 2004. The vertical and upper curved sections were drilled and lined through the problematic shale member without major stability problems. The top of the targeted Monterey was thought to be seen at the expected TVD of 10,000 ft where the 7-in. liner was set at a 60{sup o} hole angle. Significant oil and gas shows suggested the fractured interval anticipated at the heel location had been penetrated. A total of 2572 ft of 6 1/8-in. near-horizontal interval was placed in the shale section, extending planned well length by approximately 470 ft. Very little hydrocarbon in-flow was observed from fractures along the productive interval. This may be a result of the well trajectory falling underneath the Monterey fractured zone. Hydrocarbon observations, cuttings analysis and gamma-ray response indicated additional fractured intervals were accessed along the last {+-}900 ft of well length. The well was completed with a 2 7/8-in. tubing string set in a production packer in preparation for flow and swab tests to be conducted later by a service rig. The planned well time was estimated as 39 days and overall cost as $2.4 million. The actual results are 66 days at a total cost of $3.4 million. Well productivity responses during subsequent flow and swabbing tests were negative. The well failed to inflow and only minor amounts (a few barrels) of light oil were recovered. The lack of production may suggest that actual sustainable reservoir pressure is far less than anticipated. Temblor is currently planning to re-enter and clean out the well and run an Array Induction log (primarily for resistivity and correlation purposes), and an FMI log (for fracture detection). Depending on the results of these logs, an acidizing or re-drill program will be planned.« less

  10. Shift work at a modern offshore drilling rig.

    PubMed

    Rodrigues, V F; Fischer, F M; Brito, M J

    2001-12-01

    The oil and gas exploration and production offshore units are classified as hazardous installations. Work in these facilities is complex, confined and associated with a wide range of risks. The continuous operation is secured by various shift work patterns. The objective of this study was to evaluate how offshore drilling workers perceived shift work at high seas and its impacts on their life and working conditions. The main features of the studied offshore shift work schedules are: long time on board (14 to 28 days), extended shifts (12 hours or more per day), slow rotation (7 to 14 days in the same shift), long sequence of days on the night shift (7 to 14 days in a row) and the extra-long extended journey (18 hours) on shift change and landing days. Interviews revealed a wide range of stressors caused by the offshore shift work, as well as difficulties to conciliate work with family life. It was observed that changes of the family model, leading to role conflicts and social isolation, work in a hazardous environment, perceiving poor sleep when working at night shifts and the imbalance between the expected and actual rewards are the major stressors for the offshore drilling workers.

  11. SOLID2: An Antibody Array-Based Life-Detector Instrument in a Mars Drilling Simulation Experiment (MARTE)

    NASA Astrophysics Data System (ADS)

    Parro, Víctor; Fernández-Calvo, Patricia; Rodríguez Manfredi, José A.; Moreno-Paz, Mercedes; Rivas, Luis A.; García-Villadangos, Miriam; Bonaccorsi, Rosalba; González-Pastor, José Eduardo; Prieto-Ballesteros, Olga; Schuerger, Andrew C.; Davidson, Mark; Gómez-Elvira, Javier; Stoker, Carol R.

    2008-10-01

    A field prototype of an antibody array-based life-detector instrument, Signs Of LIfe Detector (SOLID2), has been tested in a Mars drilling mission simulation called MARTE (Mars Astrobiology Research and Technology Experiment). As one of the analytical instruments on the MARTE robotic drilling rig, SOLID2 performed automatic sample processing and analysis of ground core samples (0.5 g) with protein microarrays that contained 157 different antibodies. Core samples from different depths (down to 5.5 m) were analyzed, and positive reactions were obtained in antibodies raised against the Gram-negative bacterium Leptospirillum ferrooxidans, a species of the genus Acidithiobacillus (both common microorganisms in the Río Tinto area), and extracts from biofilms and other natural samples from the Río Tinto area. These positive reactions were absent when the samples were previously subjected to a high-temperature treatment, which indicates the biological origin and structural dependency of the antibody-antigen reactions. We conclude that an antibody array-based life-detector instrument like SOLID2 can detect complex biological material, and it should be considered as a potential analytical instrument for future planetary missions that search for life.

  12. SOLID2: an antibody array-based life-detector instrument in a Mars Drilling Simulation Experiment (MARTE).

    PubMed

    Parro, Víctor; Fernández-Calvo, Patricia; Rodríguez Manfredi, José A; Moreno-Paz, Mercedes; Rivas, Luis A; García-Villadangos, Miriam; Bonaccorsi, Rosalba; González-Pastor, José Eduardo; Prieto-Ballesteros, Olga; Schuerger, Andrew C; Davidson, Mark; Gómez-Elvira, Javier; Stoker, Carol R

    2008-10-01

    A field prototype of an antibody array-based life-detector instrument, Signs Of LIfe Detector (SOLID2), has been tested in a Mars drilling mission simulation called MARTE (Mars Astrobiology Research and Technology Experiment). As one of the analytical instruments on the MARTE robotic drilling rig, SOLID2 performed automatic sample processing and analysis of ground core samples (0.5 g) with protein microarrays that contained 157 different antibodies. Core samples from different depths (down to 5.5 m) were analyzed, and positive reactions were obtained in antibodies raised against the Gram-negative bacterium Leptospirillum ferrooxidans, a species of the genus Acidithiobacillus (both common microorganisms in the Río Tinto area), and extracts from biofilms and other natural samples from the Río Tinto area. These positive reactions were absent when the samples were previously subjected to a high-temperature treatment, which indicates the biological origin and structural dependency of the antibody-antigen reactions. We conclude that an antibody array-based life-detector instrument like SOLID2 can detect complex biological material, and it should be considered as a potential analytical instrument for future planetary missions that search for life.

  13. Damage to offshore infrastructure in the Gulf of Mexico by hurricanes Katrina and Rita

    NASA Astrophysics Data System (ADS)

    Cruz, A. M.; Krausmann, E.

    2009-04-01

    The damage inflicted by hurricanes Katrina and Rita to the Gulf-of-Mexico's (GoM) oil and gas production, both onshore and offshore, has shown the proneness of industry to Natech accidents (natural hazard-triggered hazardous-materials releases). In order to contribute towards a better understanding of Natech events, we assessed the damage to and hazardous-materials releases from offshore oil and natural-gas platforms and pipelines induced by hurricanes Katrina and Rita. Data was obtained through a review of published literature and interviews with government officials and industry representatives from the affected region. We also reviewed over 60,000 records of reported hazardous-materials releases from the National Response Center's (NRC) database to identify and analyze the hazardous-materials releases directly attributed to offshore oil and gas platforms and pipelines affected by the two hurricanes. Our results show that hurricanes Katrina and Rita destroyed at least 113 platforms, and severely damaged at least 53 others. Sixty percent of the facilities destroyed were built 30 years ago or more prior to the adoption of the more stringent design standards that went into effect after 1977. The storms also destroyed 5 drilling rigs and severely damaged 19 mobile offshore drilling units (MODUs). Some 19 MODUs lost their moorings and became adrift during the storms which not only posed a danger to existing facilities but the dragging anchors also damaged pipelines and other infrastructure. Structural damage to platforms included toppling of sections, and tilting or leaning of platforms. Possible causes for failure of structural and non-structural components of platforms included loading caused by wave inundation of the deck. Failure of rigs attached to platforms was also observed resulting in significant damage to the platform or adjacent infrastructure, as well as damage to equipment, living quarters and helipads. The failures are attributable to tie-down components and occurred on both fixed and floating platforms. The total number of pipelines damaged by Hurricanes Katrina and Rita as of May 1, 2006, was 457. Pipeline damage was mostly caused by damage or failure of the host platform or its development and production piping, the impact of dragging and displaced objects, and pipeline interaction at a crossing. Damage to pipelines was a major contributing factor in delaying start up of offshore oil and gas production. During our analysis of the NRC database we identified 611 reported hazardous-materials releases directly attributed to offshore platforms and pipelines affected by the two hurricanes. There were twice as many releases during Hurricane Katrina than during Rita; 80% or more of the releases reported in the NRC database occurred from platforms. Our analysis suggests that the majority of releases were petroleum products, such as crude oil and condensate, followed by natural gas. In both Katrina and Rita, releases were more likely in the front, right quadrant of the storm. Storm-surge values were highest closer to the coastline. This may help explain the higher number of releases in shallow waters. The higher number of hazardous-materials releases from platforms during Katrina may partly be attributed to the higher wind speeds for this storm as it approached land.

  14. Interlake production established using quantitative hydrocarbon well-log analysis

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lancaster, J.; Atkinson, A.

    1988-07-01

    Production was established in a new pay zone of the basal Interlake Formation adjacent to production in Midway field in Williams County, North Dakota. Hydrocarbon saturation, which was computed using hydrocarbon well-log (mud-log) data, and computed permeability encouraged the operator to run casing and test this zone. By use of drilling rig parameters, drilling mud properties, hydrocarbon-show data from the mud log, drilled rock and porosity descriptions, and wireline log porosity, this new technique computes oil saturation (percent of porosity) and permeability to the invading filtrate, using the Darcy equation. The Leonardo Fee well was drilled to test the Devonianmore » Duperow, the Silurian upper Interlake, and the Ordovician Red River. The upper two objectives were penetrated downdip from Midway production and there were no hydrocarbon shows. It was determined that the Red River was tight, based on sample examination by well site personnel. The basal Interlake, however, liberated hydrocarbon shows that were analyzed by this new technology. The results of this evaluation accurately predicted this well would be a commercial success when placed in production. Where geophysical log analysis might be questionable, this new evaluation technique may provide answers to anticipated oil saturation and producibility. The encouraging results of hydrocarbon saturation and permeability, produced by this technique, may be largely responsible for the well being in production today.« less

  15. Risk Management at NASA and Its Applicability to the Oil and Gas Industry

    NASA Technical Reports Server (NTRS)

    Kaplan, David

    2018-01-01

    NASA has a world-class capability for quantitatively assessing the risk of highly-complex, isolated engineering structures operated in extremely hostile environments. In particular, the International Space Station (ISS) represents a reasonable risk analog for High Pressure, High Temperature drilling and production operations on deepwater rigs. Through a long-term U.S. Government Interagency Agreement, BSEE has partnered with NASA to modify NASA's Probabilistic Risk Assessment (PRA) capabilities for application to deepwater drilling and production operations. The immediate focus of the activity will be to modify NASA PRA Procedure Guides and Methodology Documents to make them applicable to the Oil &Gas Industry. The next step will be for NASA to produce a PRA for a critical drilling system component, such as a Blowout Preventer (BOP). Subsequent activities will be for NASA and industry partners to jointly develop increasingly complex PRA's that analyze other critical drilling and production system components, including both hardware and human reliability. In the presentation, NASA will provide the objectives, schedule, and current status of its PRA activities for BSEE. Additionally, NASA has a Space Act Agreement with Anadarko Petroleum Corporation to develop a PRA for a generic 20K BOP. NASA will summarize some of the preliminary insights gained to date from that 20K BOP PRA as an example of the distinction between quantitative versus qualitative risk assessment.

  16. The Yucca Mountain Project prototype air-coring test, U12g tunnel, Nevada test site

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ray, J.M.; Newsom, J.C.

    1994-12-01

    The Prototype Air-Coring Test was conducted at the Nevada Test Site (NTS) G-Tunnel facility to evaluate standard coring techniques, modified slightly for air circulation, for use in testing at a prospective nuclear waste repository at Yucca Mountain, Nevada. Air-coring technology allows sampling of subsurface lithology with minimal perturbation to ambient characteristic such as that required for exploratory holes near aquifers, environmental applications, and site characterization work. Two horizontal holes were cored, one 50 ft long and the other 150 ft long, in densely welded fractured tuff to simulate the difficult drilling conditions anticipated at Yucca Mountain. Drilling data from sevenmore » holes on three other prototype tests in nonwelded tuff were also collected for comparison. The test was used to establish preliminary standards of performance for drilling and dust collection equipment and to assess procedural efficiencies. The Longyear-38 drill achieved 97% recovery for HQ-size core (-2.5 in.), and the Atlas Copco dust collector (DCT-90) captured 1500 lb of fugitive dust in a mine environment with only minor modifications. Average hole production rates were 6-8 ft per 6-h shift in welded tuff and almost 20 ft per shift on deeper holes in nonwelded tuff. Lexan liners were successfully used to encapsulate core samples during the coring process and protect core properties effectively. The Prototype Air-Coring Test demonstrated that horizontal air coring in fractured welded tuff (to at least 150 ft) can be safely accomplished by proper selection, integration, and minor modification of standard drilling equipment, using appropriate procedures and engineering controls. The test also indicated that rig logistics, equipment, and methods need improvement before attempting a large-scale dry drilling program at Yucca Mountain.« less

  17. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    Ghana National Petroleum Corp. (GNPC) plans a two well offshore drilling program it hopes will lead to a resumption of hydrocarbon production in the West African state. The wells will be drilled in South Tano field in the extreme western sector of Ghana's offshore area, near the boundary with Ivory Coast. If the program is successful, the state company will develop a novel floating production system to handle and export oil. Gas will provide fuel for an electrical power generating unit integrated into a floating production system. Power will move ashore through a submarine cable. North and south Tano fieldsmore » were discovered by Phillips Petroleum Corp., which relinquished the acreage in 1982. The South Tano discovery well flowed 1,614 b/d of oil and 8.2 MMCfd of gas. Studies by a unit of ARCO, when it was a partner in a group that later acquired the Tano block, pegged North Tano hydrocarbons in place at 53.6 million bbl of oil and 102 bcf of gas. Braspetro, under contract with GNPC, estimated South Tano hydrocarbons in place at 82 million bbl of oil and 100 bcf of gas. GNPC is evaluating the possibility of rehabilitating Saltpond oil field about 150 miles east-northeast of North and South Tano. Saltpond has been shut in since 1985.« less

  18. Monitoring Well Installation and Groundwater Sampling and Analysis Plan at the USARC Training Reserve, 84th Division, Milwaukee, Wisconsin

    DTIC Science & Technology

    1988-11-01

    paint chips at the sampling site. 0 Clean water tanks, pumps, mud pans, hoses, including hoses and tanks used to transfer water from source to drill rig...TO’ LCA , Filll I F’APCr,;I~- € C/ " rKL2PIrlA , ATTFNrIGN TO SMOKING. ALCOHOLF MFDrICATIONP AND FXPOSI.RE TO CARCINOGENS.1 ENERAL MEDICAl. HISTORY...A. General: 1. Place samples in core trough for visual inspection. After logging, place selected samples in sample jars or wood core boxes. 2. Seal

  19. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bryngelson, R.H.

    Describes installation of 3 satellite subsea trees in 500 ft of water from a semisubmersible drilling rig. These wet, diver-assist trees are part of Phillips Petroleum's early development and production program (EDPP) for the Espoir field offshore Ivory Coast, with plans calling for 5 satellite wells with downhole completion equipment and subsea production trees. Diagram shows how a converted jackup, Dan Duke, supports equipment to handle production from subsea wells. Table gives time breakdown of subsea tree installation. Before mobilizing the subsea trees, control system, and tubulars to the rig, a study of deck layout, payloads, and traffic patterns wasmore » performed. Concludes that, based on experience in this project and the cost differences between purchase and installation costs, final success is 90% dependent on informed and trained field personnel after engineering, design, and manufacturing; attention to installation procedures and training of field and operational personnel are as critical or more critical than design changes to equipment; and selection of a supplier for high technology equipment, based on a low bid alone, may not translate into lower installation costs.« less

  20. Evaluation of haptic interfaces for simulation of drill vibration in virtual temporal bone surgery.

    PubMed

    Ghasemloonia, Ahmad; Baxandall, Shalese; Zareinia, Kourosh; Lui, Justin T; Dort, Joseph C; Sutherland, Garnette R; Chan, Sonny

    2016-11-01

    Surgical training is evolving from an observership model towards a new paradigm that includes virtual-reality (VR) simulation. In otolaryngology, temporal bone dissection has become intimately linked with VR simulation as the complexity of anatomy demands a high level of surgeon aptitude and confidence. While an adequate 3D visualization of the surgical site is available in current simulators, the force feedback rendered during haptic interaction does not convey vibrations. This lack of vibration rendering limits the simulation fidelity of a surgical drill such as that used in temporal bone dissection. In order to develop an immersive simulation platform capable of haptic force and vibration feedback, the efficacy of hand controllers for rendering vibration in different drilling circumstances needs to be investigated. In this study, the vibration rendering ability of four different haptic hand controllers were analyzed and compared to find the best commercial haptic hand controller. A test-rig was developed to record vibrations encountered during temporal bone dissection and a software was written to render the recorded signals without adding hardware to the system. An accelerometer mounted on the end-effector of each device recorded the rendered vibration signals. The newly recorded vibration signal was compared with the input signal in both time and frequency domains by coherence and cross correlation analyses to quantitatively measure the fidelity of these devices in terms of rendering vibrotactile drilling feedback in different drilling conditions. This method can be used to assess the vibration rendering ability in VR simulation systems and selection of ideal haptic devices. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Influence of drilling operations on drilling mud gas monitoring during IODP Exp. 338 and 348

    NASA Astrophysics Data System (ADS)

    Hammerschmidt, Sebastian; Toczko, Sean; Kubo, Yusuke; Wiersberg, Thomas; Fuchida, Shigeshi; Kopf, Achim; Hirose, Takehiro; Saffer, Demian; Tobin, Harold; Expedition 348 Scientists, the

    2014-05-01

    The history of scientific ocean drilling has developed some new techniques and technologies for drilling science, dynamic positioning being one of the most famous. However, while industry has developed newer tools and techniques, only some of these have been used in scientific ocean drilling. The introduction of riser-drilling, which recirculates the drilling mud and returns to the platform solids and gases from the formation, to the International Ocean Drilling Program (IODP) through the launch of the Japan Agency of Marine Earth-Science and Technology (JAMSTEC) riser-drilling vessel D/V Chikyu, has made some of these techniques available to science. IODP Expedition 319 (NanTroSEIZE Stage 2: riser/riserless observatory) was the first such attempt, and among the tools and techniques used was drilling mud gas analysis. While industry regularly conducts drilling mud gas logging for safety concerns and reservoir evaluation, science is more interested in other components (e.g He, 222Rn) that are beyond the scope of typical mud logging services. Drilling mud gas logging simply examines the gases released into the drilling mud as part of the drilling process; the bit breaks and grinds the formation, releasing any trapped gases. These then circulate within the "closed circuit" mud-flow back to the drilling rig, where a degasser extracts these gases and passes them on to a dedicated mud gas logging unit. The unit contains gas chromatographs, mass spectrometers, spectral analyzers, radon gas analyzers, and a methane carbon isotope analyzer. Data are collected and stored in a database, together with several drilling parameters (rate of penetration, mud density, etc.). This initial attempt was further refined during IODP Expeditions 337 (Deep Coalbed Biosphere off Shimokita), 338 (NanTroSEIZE Stage 3: NanTroSEIZE Plate Boundary Deep Riser 2) and finally 348 (NanTroSEIZE Stage 3: NanTroSEIZE Plate Boundary Deep Riser 3). Although still in its development stage for scientific application, this technique can provide a valuable suite of measurements to complement more traditional IODP shipboard measurements. Here we present unpublished data from IODP Expeditions 338 and 348, penetrating the Nankai Accretionary wedge to 3058.5 meters below seafloor. Increasing mud density decreased degasser efficiency, especially for higher hydrocarbons. Blurring of the relative variations in total gas by depth was observed, and confirmed with comparison to headspace gas concentrations from the cored interval. Theoretically, overpressured zones in the formation can be identified through C2/C3 ratios, but these ratios are highly affected by changing drilling parameters. Proper mud gas evaluations will need to carefully consider the effects of variable drilling parameters when designing experiments and interpreting the data.

  2. USE OF CUTTING-EDGE HORIZONTAL AND UNDERBALANCED DRILLING TECHNOLOGIES AND SUBSURFACE SEISMIC TECHNIQUES TO EXPLORE, DRILL AND PRODUCE RESERVOIRED OIL AND GAS FROM THE FRACTURED MONTEREY BELOW 10,000 FT IN THE SANTA MARIA BASIN OF CALIFORNIA

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    George Witter; Robert Knoll; William Rehm

    2005-02-01

    This project was undertaken to demonstrate that oil and gas can be drilled and produced safely and economically from a fractured Monterey reservoir in the Santa Maria Basin of California by employing horizontal wellbores and underbalanced drilling technologies. Two vertical wells were previously drilled in this area by Temblor Petroleum with heavy mud and conventional completions; neither was commercially productive. A new well was drilled by the project team in 2004 with the objective of accessing an extended length of oil-bearing, high-resistivity Monterey shale via a horizontal wellbore, while implementing managed-pressure drilling (MPD) techniques to avoid formation damage. Initial projectmore » meetings were conducted in October 2003. The team confirmed that the demonstration well would be completed open-hole to minimize productivity impairment. Following an overview of the geologic setting and local field experience, critical aspects of the application were identified. At the pre-spud meeting in January 2004, the final well design was confirmed and the well programming/service company requirements assigned. Various design elements were reduced in scope due to significant budgetary constraints. Major alterations to the original plan included: (1) a VSP seismic survey was delayed to a later phase; (2) a new (larger) surface hole would be drilled rather than re-enter an existing well; (3) a 7-in. liner would be placed into the top of the Monterey target as quickly as possible to avoid problems with hole stability; (4) evaluation activities were reduced in scope; (5) geosteering observations for fracture access would be deduced from penetration rate, cuttings description and hydrocarbon in-flow; and (6) rather than use nitrogen, a novel air-injection MPD system was to be implemented. Drilling operations, delayed from the original schedule by capital constraints and lack of rig availability, were conducted from September 12 to November 11, 2004. The vertical and upper curved sections were drilled and lined through the problematic shale member without major stability problems. The top of the targeted Monterey was thought to be seen at the expected TVD of 10,000 ft where the 7-in. liner was set at a 60{sup o} hole angle. Significant oil and gas shows suggested the fractured interval anticipated at the heel location had been penetrated. A total of 2572 ft of 6.-in. near-horizontal interval was placed in the shale section, extending planned well length by approximately 470 ft. Very little hydrocarbon in-flow was observed from fractures along the productive interval. This may be a result of the well trajectory falling underneath the Monterey fractured zone. Hydrocarbon observations, cuttings analysis and gamma-ray response indicated additional fractured intervals were accessed along the last {+-}900 ft of well length. The well was completed with a 2 7/8-in. tubing string set in a production packer in preparation for flow and swab tests to be conducted later by a service rig. The planned well time was estimated as 39 days and overall cost as $2.4 million. The actual results are 66 days at a total cost of $3.4 million. Well productivity responses during subsequent flow and swabbing tests were negative. The well failed to inflow and only minor amounts (a few barrels) of light oil were recovered. The lack of production may suggest that actual sustainable reservoir pressure is far less than anticipated. Temblor is currently investigating the costs and operational viability of re-entering the well and conducting an FMI (fracture detection) log and/or an acid stimulation. No final decision or detailed plans have been made regarding these potential interventions at this time.« less

  3. Optimizing Drilling Efficiency by PWD (Pressure-While-Drilling) Sensor in wells which were drilled in the Khazar-Caspian Sea of the Azerbaijan Republic

    NASA Astrophysics Data System (ADS)

    Amirov, Elnur

    2017-04-01

    Sperry Drilling Services' PWD sensor improve and support drilling efficiency by providing very important, real-time downhole pressure information that allows to make faster and better drilling decisions. The PWD service, provides accurate annular pressure, internal pressure and temperature measurements using any of well-known telemetry systems: positive mud pulse, negative mud pulse and electromagnetic. Pressure data can be transmitted in real time and recorded in downhole memory. In the pumpsoff mode, the minimum, maximum and average pressures observed during the non-circulating period are transmitted via mud pulse telemetry when circulation recommences. These measurements provide the knowledge to avoid lost circulation and detect flow/kicks before they happen. The PWD sensor also reduces the risk of problems related by unexpected fracture or collapse. Sperry's PWD sensor also helps to avoid lost circulation and flow/kick, which can lead to costly delays in drilling. Annular pressure increases often reflect ineffective cuttings removal and poor hole cleaning, both of which can lead to lost circulation. The PWD sensor detects the increase and drilling fluid parameters and operating procedures can be modified to improve hole-cleaning efficiency. On extended reach wells, real-time information helps to maintain wellbore pressures between safe operating limits and to monitor hole cleaning. The PWD sensor also provides early detection of well flows and kicks. A drop in pressure, can indicate gas, oil and water kicks. Because the sensor is making its measurement downhole, the PWD sensor makes it possible to detect such pressure drops earlier than more traditional surface measurements. The PWD sensor has high-accuracy quartz gauges and is able to record data because of its battery-powered operation. It is also extremely useful in specialized drilling environments, such as high-pressure/high-temperature, extended-reach and deepwater wells. When combined with the rig management system, surface and downhole measurements, can be compared for more accurate and extensive analysis. PWD sensor was utilized with encouraging results in many wells up to 3000-6000m subsurface reservoirs (these wells were drilled in the Khazar-Caspian region of the Azerbaijan Republic) and acquired PWD RT/RM data implemented for best drilling practices in other brand new drilled offset wells in order to help us achieve our mission to drill safe, faster, on target, optimize drilling efficiency, maximize well value and reservoir insight.

  4. Implementation of improved underbalanced drilling in AbuDhabi onshore field

    NASA Astrophysics Data System (ADS)

    Alhammadi, Adel Mohammed

    Abu Dhabi Company for Onshore Oil Operations (ADCO) is considering Underbalanced Drilling (UBD) as a means to develop lower permeability units in its fields. In addition to productivity and recovery gains, ADCO also expects reservoir characterization benefits from UBD. Reservoir screening studies were carried out on all of ADCO's reservoirs to determine their applicability for UBD. The primary business benefits of UBD were determined to be reservoir characterization, damage Mitigation, and rate of Penetration "ROP" Improvement. Apart from the primary benefits, some of the secondary benefits of UBD that were identified beforehand included rig performance. Since it's a trial wells, the challenge was to drill these wells safely, efficiently and of course meeting well objectives. Many operators worldwide drill these well in underbalanced mode but complete it overbalanced. In our case the plan was to drill and complete these wells in underbalanced condition. But we had to challenge most operators and come up with special and unique casing hanger design to ensure well control barriers exists while fishing the control line of the Downhole Deployment Valve "DDV". After intensive studies and planning, the hanger was designed as per our recommendations and found to be effective equipment that optimized the operational time and the cost as well. This report will provide better understanding of UBD technique in general and shade on the special designed casing hanger compared to conventional or what's most used worldwide. Even thought there were some issues while running the casing hanger prior drilling but managed to capture the learning's from each well and re-modified the hanger and come up with better deign for the future wells. Finally, the new design perform a good performance of saving the operation time and assisting the project to be done in a safe and an easy way without a major impact on the well cost. This design helped to drill and complete these wells safely with requirement to kill the wells and this ensured least reservoir damage.

  5. Investigating the effects of PDC cutters geometry on ROP using the Taguchi technique

    NASA Astrophysics Data System (ADS)

    Jamaludin, A. A.; Mehat, N. M.; Kamaruddin, S.

    2017-10-01

    At times, the polycrystalline diamond compact (PDC) bit’s performance dropped and affects the rate of penetration (ROP). The objective of this project is to investigate the effect of PDC cutter geometry and optimize them. An intensive study in cutter geometry would further enhance the ROP performance. The relatively extended analysis was carried out and four significant geometry factors have been identified that directly improved the ROP. Cutter size, back rake angle, side rake angle and chamfer angle are the stated geometry factors. An appropriate optimization technique that effectively controls all influential geometry factors during cutters manufacturing is introduced and adopted in this project. By adopting L9 Taguchi OA, simulation experiment is conducted by using explicit dynamics finite element analysis. Through a structure Taguchi analysis, ANOVA confirms that the most significant geometry to improve ROP is cutter size (99.16% percentage contribution). The optimized cutter is expected to drill with high ROP that can reduce the rig time, which in its turn, may reduce the total drilling cost.

  6. Industry trends point toward bright future

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kliewer, G.

    Examination of the trends for the past 5 yr shows increasing soundness in the international petroleum industry. U.S. figures alone have a mixed complexion, with imports, exports, crude prices, and demand rising steadily. U.S. crude oil production, however, increased through 1970, but leveled off last year. Decreases occurred during 1971 in the number of rigs working, gas producers, and wildcats drilled. Trends in Free World drilling and production (outside the U.S. and Canada) have been upward and are expected to continue expanding generally through the year 2000. World petroleum demand, the key to this activity, has risen steadily, and allmore » forecasts predict increases of around 3.5% a yr for the next 15 yr. Included in this outlook is a booming natural gas use, which comes as a result of worldwide emphasis on clean-burning, nonpolluting fuels. Competition among energy sources may not materialize. Instead, a mix of all available energy sources will be needed to meet the demand. Tabular data provide complete details.« less

  7. Exploration momentum builds in offshore Indonesia

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Van Dyke, L.F.

    1969-06-23

    A base map shows where the major action is taking place in SE. Asian waters. Eight rigs are active in the Indonesian-Malaysian theater. Several companies or groups have plans in various stages of preliminary exploration, from running seismic surveys to charting exploration programs. The Indonesian Government appears willing to negotiate new production sharing agreements for blocks of open acreage. Malaya would also like to grant some new acreage. Only 3 companies have done significant drilling to date. Production in one wildcat, was estimated at about 2,480 bpd, but the well was lost due to mechanical problems. The second well alsomore » found some oil, but was not completed due to mechanical troubles. A third test is reported to have encountered the same productive limestones. A total of 3 dry holes have been drilled off S. Kalimantan. A discovery well, some 80 miles northeast of Djakarta, and about 18 miles off shore in the Java Sea, flowed 2,600 bpd.« less

  8. Warm water and life beneath the grounding zone of an Antarctic outlet glacier

    NASA Astrophysics Data System (ADS)

    Sugiyama, Shin; Sawagaki, Takanobu; Fukuda, Takehiro

    2013-04-01

    Ice-ocean interaction plays a key role in rapidly changing Antarctic ice sheet margins. Recent studies demonstrated that warming ocean is eroding floating part of the ice sheet, resulting in thinning, retreat and acceleration of ice shelves and outlet glaciers. Field data are necessary to understand such processes, but direct observations at the interface of ice and the ocean are lacking, particularly beneath the grounding zone. To better understand the interaction of Antarctic ice sheet and the ocean, we performed subglacial measurements through boreholes drilled in the grounding zone of Langhovde Glacier, an outlet glacier in East Antarctica. Langhovde Glacier is located at 69°12'S, 39°48'E, approximately 20 km south of a Japanese research station Syowa. The glacier discharges ice into Lützow-holm Bay through a 3-km-wide floating terminus at a rate of 130 m a-1. Fast flowing feature is confined by bedrock to the west and slow moving ice to the east, and it extends about 10 km upglacier from the calving front. In 2011/12 austral summer season, we operated a hot water drilling system to drill through the glacier at 2.5 and 3 km from the terminus. Inspections of the boreholes revealed the ice was underlain by a shallow saline water layer. Ice and water column thicknesses were found to be 398 and 24 m at the first site, and 431 and 10 m at the second site. Judging from ice surface and bed elevations, the drilling sites were situated at within a several hundred meters from the grounding line. Sensors were lowered into the boreholes to measure temperature, salinity and current within the subglacial water layer. Salinity and temperature from the two sites were fairly uniform (34.25±0.05 PSU and -1.45±0.05°C), indicating vertical and horizontal mixing in the layer. The measured temperature was >0.7°C warmer than the in-situ freezing point, and very similar to the values measured in the open ocean near the glacier front. Subglacial current was up to 3 cm/s, which is sufficient to carry coastal water to the study sites within several days. A video camera suspended in the boreholes captured a crustacean and krill beneath the grounding zone. Subglacial water samples contained abundant phytoplankton, which were most likely transported from the open ocean and served as trophic resources to the animals living under >400 m thick glacier. Our observations indicate that warm coastal water is actively transported to the grounding zone by subshelf current, and efficiently melting the floating ice bottom. It is also implied that changes in the ocean would immediately reach and influence physical and biological environment beneath the grounding zone.

  9. Final report - Magma Energy Research Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Colp, J.L.

    1982-10-01

    Scientific feasibility was demonstrated for the concept of magma energy extraction. The US magma resource is estimated at 50,000 to 500,000 quads of energy - a 700- to 7000-yr supply at the current US total energy use rate of 75 quads per year. Existing geophysical exploration systems are believed capable of locating and defining magma bodies and were demonstrated over a known shallow buried molten-rock body. Drilling rigs that can drill to the depths required to tap magma are currently available and experimental boreholes were drilled well into buried molten rock at temperatures up to 1100/sup 0/C. Engineering materials compatiblemore » with the buried magma environment are available and their performances were demonstrated in analog laboratory experiments. Studies show that energy can be extracted at attractive rates from magma resources in all petrologic compositions and physical configurations. Downhole heat extraction equipment was designed, built, and demonstrated successfully in buried molten rock and in the very hot margins surrounding it. Two methods of generating gaseous fuels in the high-temperature magmatic environment - generation of H/sub 2/ by the interaction of water with the ferrous iron and H/sub 2/, CH/sub 4/, and CO generation by the conversion of water-biomass mixtures - have been investigated and show promise.« less

  10. Performance of hydraulic fracturing and matrix acidizing in horizontal wellbores -- Offshore Qatar

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Edwards, M.G.R.; Pongratz, R.

    Considerable debate in the Middle East has centered upon what was previously felt to be two separate methods of enhancing revenues and daily production; hydraulic fracturing and horizontal drilling. In an effort to maximize return on investment, these two issues have been successfully combined in other areas of the world. In order to establish the suitability of this technology in this area, two horizontal wells with over 3,050m (10,000ft) of lateral section were drilled into the Cretaceous Kharaib formation, overlying the North Field, Offshore Qatar. A massive stimulation program was performed in order to evaluate the most feasible stimulation methodmore » from both a technical and economical perspective for further field development considerations.Three propped hydraulic fracturing treatments were performed using 183, 500kg (403, 700lb) of 20/40 mesh sand, and seventeen acid matrix treatments placing over 3,217,250l (850,000gals) of HCL into the lateral sections of both wells. This paper describes the performance, operation and logistical support required to complete this offshore operation with join a minimal time frame. The use of a mobile offshore jack-up platform, whereby a land based fracturing spread was placed onto the deck of a converted drilling rig is described.« less

  11. Facility for testing ice drills

    NASA Astrophysics Data System (ADS)

    Nielson, Dennis L.; Delahunty, Chris; Goodge, John W.; Severinghaus, Jeffery P.

    2017-05-01

    The Rapid Access Ice Drill (RAID) is designed for subsurface scientific investigations in Antarctica. Its objectives are to drill rapidly through ice, to core samples of the transition zone and bedrock, and to leave behind a borehole observatory. These objectives required the engineering and fabrication of an entirely new drilling system that included a modified mining-style coring rig, a unique fluid circulation system, a rod skid, a power unit, and a workshop with areas for the storage of supplies and consumables. An important milestone in fabrication of the RAID was the construction of a North American Test (NAT) facility where we were able to test drilling and fluid processing functions in an environment that is as close as possible to that expected in Antarctica. Our criteria for site selection was that the area should be cold during the winter months, be located in an area of low heat flow, and be at relatively high elevation. We selected a site for the facility near Bear Lake, Utah, USA. The general design of the NAT well (NAT-1) started with a 27.3 cm (10.75 in.) outer casing cemented in a 152 m deep hole. Within that casing, we hung a 14 cm (5.5 in.) casing string, and, within that casing, a column of ice was formed. The annulus between the 14 and 27.3 cm casings provided the path for circulation of a refrigerant. After in-depth study, we chose to use liquid CO2 to cool the hole. In order to minimize the likelihood of the casing splitting due to the volume increase associated with freezing water, the hole was first cooled and then ice was formed in increments from the bottom upward. First, ice cubes were placed in the inner liner and then water was added. Using this method, a column of ice was incrementally prepared for drilling tests. The drilling tests successfully demonstrated the functioning of the RAID system. Reproducing such a facility for testing of other ice drilling systems could be advantageous to other research programs in the future.

  12. The effects of the Yogyakarta earthquake at LUSI mud volcano, Indonesia

    NASA Astrophysics Data System (ADS)

    Lupi, M.; Saenger, E. H.; Fuchs, F.; Miller, S. A.

    2013-12-01

    The M6.3 Yogyakarta earthquake shook Central Java on May 27th, 2006. Forty seven hours later, hot mud outburst at the surface near Sidoarjo, approximately 250 km from the earthquake epicentre. The mud eruption continued and originated LUSI, the youngest mud volcanic system on earth. Since the beginning of the eruption, approximately 30,000 people lost their homes and 13 people died due to the mud flooding. The causes that initiated the eruption are still debated and are based on different geological observations. The earthquake-triggering hypothesis is supported by the evidence that at the time of the earthquake ongoing drilling operations experienced a loss of the drilling mud downhole. In addition, the eruption of the mud began only 47 hours after the Yogyakarta earthquake and the mud reached the surface at different locations aligned along the Watukosek fault, a strike-slip fault upon which LUSI resides. Moreover, the Yogyakarta earthquake also affected the volcanic activity of Mt. Semeru, located as far as Lusi from the epicentre of the earthquake. However, the drilling-triggering hypothesis points out that the earthquake was too far from LUSI for inducing relevant stress changes at depth and highlight how upwelling fluids that reached the surface first emerged only 200 m far from the drilling rig that was operative at the time. Hence, was LUSI triggered by the earthquake or by drilling operations? We conducted a seismic wave propagation study on a geological model based on vp, vs, and density values for the different lithologies and seismic profiles of the crust beneath LUSI. Our analysis shows compelling evidence for the effects produced by the passage of seismic waves through the geological formations and highlights the importance of the overall geological structure that focused and reflected incoming seismic energy.

  13. Shipboard Analytical Capabilities on the Renovated JOIDES Resolution, IODP Riserless Drilling Vessel

    NASA Astrophysics Data System (ADS)

    Blum, P.; Foster, P.; Houpt, D.; Bennight, C.; Brandt, L.; Cobine, T.; Crawford, W.; Fackler, D.; Fujine, K.; Hastedt, M.; Hornbacher, D.; Mateo, Z.; Moortgat, E.; Vasilyev, M.; Vasilyeva, Y.; Zeliadt, S.; Zhao, J.

    2008-12-01

    The JOIDES Resolution (JR) has conducted 121 scientific drilling expeditions during the Ocean Drilling Program (ODP) and the first phase of the Integrated Ocean Drilling Program (IODP) (1983-2006). The vessel and scientific systems have just completed an NSF-sponsored renovation (2005-2008). Shipboard analytical systems have been upgraded, within funding constraints imposed by market driven vessel conversion cost increases, to include: (1) enhanced shipboard analytical services including instruments and software for sampling and the capture of chemistry, physical properties, and geological data; (2) new data management capabilities built around a laboratory information management system (LIMS), digital asset management system, and web services; (3) operations data services with enhanced access to navigation and rig instrumentation data; and (4) a combination of commercial and home-made user applications for workflow- specific data extractions, generic and customized data reporting, and data visualization within a shipboard production environment. The instrumented data capture systems include a new set of core loggers for rapid and non-destructive acquisition of images and other physical properties data from drill cores. Line-scan imaging and natural gamma ray loggers capture data at unprecedented quality due to new and innovative designs. Many instruments used to characterize chemical compounds of rocks, sediments, and interstitial fluids were upgraded with the latest technology. The shipboard analytical environment features a new and innovative framework (DESCinfo) and application (DESClogik) for capturing descriptive and interpretive data from geological sub-domains such as sedimentology, petrology, paleontology, structural geology, stratigraphy, etc. This system fills a long-standing gap by providing a global database, controlled vocabularies and taxa name lists with version control, a highly configurable spreadsheet environment for data capture, and visualization of context data collected with the shipboard core loggers and other instruments.

  14. AURORA BOREALIS - European Research Icebreaker With Drilling Capability

    NASA Astrophysics Data System (ADS)

    Biebow, N.; Lembke-Jene, L.; Kunz-Pirrung, M.; Thiede, J.

    2008-12-01

    The polar oceans are the least known areas of the globe, in although they hold the key to many of our climate´s secrets. How does the sea ice coverage and the sea water properties change? How do plants and animals survive under the most extreme conditions of the earth? Which information of past climate change can be read from the sediments at the sea-floor and how can the future changing climate be predicted? In order to answer such and further questions, for the moment a hypermodern research vessel, the AURORA BOREALIS, is planned, which can handle the cool summers and freezing winters of the polar oceans and which can drill deep into the sea floor. AURORA BOREALIS will be the most advanced Research Icebreaker in the world with a multi-functional role of drilling in deep ocean basins and supporting climate/environmental research and decision support for stakeholder governments for the next 35-40 years. It will have a high icebreaking capacity to penetrate autonomously (single ship operation) into the central Arctic Ocean with more than 2.5 meters of ice cover, during all seasons of the year. The new technological features will include dynamic positioning in closed sea- ice cover, satellite navigation and ice-management support and the deployment and operation of Remotely Operated Vehicles (ROV) and Autonomous Underwater Vehicles (AUVs) from the twin moon-pools. A unique feature of the vessel is the deep-sea drilling rig, which will enable sampling of the ocean floor and sub-sea up to 5000 m water and 1000 m penetration at the most inhospitable places on earth. The drilling capability will be deployed in both Polar Regions on the long run and AURORA BOREALIS will be the only vessel worldwide that could undertake this type of scientific investigation.

  15. Smaller Footprint Drilling System for Deep and Hard Rock Environments; Feasibility of Ultra-High-Speed Diamond Drilling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Arnis Judzis; Alan Black; Homer Robertson

    2006-03-01

    The two phase program addresses long-term developments in deep well and hard rock drilling. TerraTek believes that significant improvements in drilling deep hard rock will be obtained by applying ultra-high rotational speeds (greater than 10,000 rpm). The work includes a feasibility of concept research effort aimed at development that will ultimately result in the ability to reliably drill ''faster and deeper'' possibly with smaller, more mobile rigs. The principle focus is on demonstration testing of diamond bits rotating at speeds in excess of 10,000 rpm to achieve high rate of penetration (ROP) rock cutting with substantially lower inputs of energymore » and loads. The significance of the ultra-high rotary speed drilling system is the ability to drill into rock at very low weights on bit and possibly lower energy levels. The drilling and coring industry today does not practice this technology. The highest rotary speed systems in oil field and mining drilling and coring today run less than 10,000 rpm--usually well below 5,000 rpm. This document details the progress to date on the program entitled ''Smaller Footprint Drilling System for Deep and Hard Rock Environments: Feasibility of Ultra-High-Speed Diamond Drilling'' for the period starting 1 October 2004 through 30 September 2005. Additionally, research activity from 1 October 2005 through 28 February 2006 is included in this report: (1) TerraTek reviewed applicable literature and documentation and convened a project kick-off meeting with Industry Advisors in attendance. (2) TerraTek designed and planned Phase I bench scale experiments. Some difficulties continue in obtaining ultra-high speed motors. Improvements have been made to the loading mechanism and the rotational speed monitoring instrumentation. New drill bit designs have been provided to vendors for production. A more consistent product is required to minimize the differences in bit performance. A test matrix for the final core bit testing program has been completed. (3) TerraTek is progressing through Task 3 ''Small-scale cutting performance tests''. (4) Significant testing has been performed on nine different rocks. (5) Bit balling has been observed on some rock and seems to be more pronounces at higher rotational speeds. (6) Preliminary analysis of data has been completed and indicates that decreased specific energy is required as the rotational speed increases (Task 4). This data analysis has been used to direct the efforts of the final testing for Phase I (Task 5). (7) Technology transfer (Task 6) has begun with technical presentations to the industry (see Judzis).« less

  16. SMALLER FOOTPRINT DRILLING SYSTEM FOR DEEP AND HARD ROCK ENVIRONMENTS; FEASIBILITY OF ULTRA-HIGH SPEED DIAMOND DRILLING

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Alan Black; Arnis Judzis

    2004-10-01

    The two phase program addresses long-term developments in deep well and hard rock drilling. TerraTek believes that significant improvements in drilling deep hard rock will be obtained by applying ultra-high (greater than 10,000 rpm) rotational speeds. The work includes a feasibility of concept research effort aimed at development and test results that will ultimately result in the ability to reliably drill ''faster and deeper'' possibly with rigs having a smaller footprint to be more mobile. The principle focus is on demonstration testing of diamond bits rotating at speeds in excess of 10,000 rpm to achieve high rate of penetration rockmore » cutting with substantially lower inputs of energy and loads. The project draws on TerraTek results submitted to NASA's ''Drilling on Mars'' program. The objective of that program was to demonstrate miniaturization of a robust and mobile drilling system that expends small amounts of energy. TerraTek successfully tested ultrahigh speed ({approx}40,000 rpm) small kerf diamond coring. Adaptation to the oilfield will require innovative bit designs for full hole drilling or continuous coring and the eventual development of downhole ultra-high speed drives. For domestic operations involving hard rock and deep oil and gas plays, improvements in penetration rates is an opportunity to reduce well costs and make viable certain field developments. An estimate of North American hard rock drilling costs is in excess of $1,200 MM. Thus potential savings of $200 MM to $600 MM are possible if drilling rates are doubled [assuming bit life is reasonable]. The net result for operators is improved profit margin as well as an improved position on reserves. The significance of the ''ultra-high rotary speed drilling system'' is the ability to drill into rock at very low weights on bit and possibly lower energy levels. The drilling and coring industry today does not practice this technology. The highest rotary speed systems in oil field and mining drilling and coring today run less than 10,000 rpm--usually well below 5,000 rpm. This document details the progress to date on the program entitled ''SMALLER FOOTPRINT DRILLING SYSTEM FOR DEEP AND HARD ROCK ENVIRONMENTS; FEASIBILITY OF ULTRA-HIGH SPEED DIAMOND DRILLING'' for the period starting June 23, 2003 through September 30, 2004. TerraTek has reviewed applicable literature and documentation and has convened a project kick-off meeting with Industry Advisors in attendance. TerraTek has designed and planned Phase I bench scale experiments. Some difficulties in obtaining ultra-high speed motors for this feasibility work were encountered though they were sourced mid 2004. TerraTek is progressing through Task 3 ''Small-scale cutting performance tests''. Some improvements over early NASA experiments have been identified.« less

  17. SMALLER FOOTPRINT DRILLING SYSTEM FOR DEEP AND HARD ROCK ENVIRONMENTS; FEASIBILITY OF ULTRA-HIGH SPEED DIAMOND DRILLING

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Alan Black; Arnis Judzis

    2004-10-01

    The two phase program addresses long-term developments in deep well and hard rock drilling. TerraTek believes that significant improvements in drilling deep hard rock will be obtained by applying ultra-high (greater than 10,000 rpm) rotational speeds. The work includes a feasibility of concept research effort aimed at development and test results that will ultimately result in the ability to reliably drill ''faster and deeper'' possibly with rigs having a smaller footprint to be more mobile. The principle focus is on demonstration testing of diamond bits rotating at speeds in excess of 10,000 rpm to achieve high rate of penetration rockmore » cutting with substantially lower inputs of energy and loads. The project draws on TerraTek results submitted to NASA's ''Drilling on Mars'' program. The objective of that program was to demonstrate miniaturization of a robust and mobile drilling system that expends small amounts of energy. TerraTek successfully tested ultrahigh speed ({approx}40,000 rpm) small kerf diamond coring. Adaptation to the oilfield will require innovative bit designs for full hole drilling or continuous coring and the eventual development of downhole ultra-high speed drives. For domestic operations involving hard rock and deep oil and gas plays, improvements in penetration rates is an opportunity to reduce well costs and make viable certain field developments. An estimate of North American hard rock drilling costs is in excess of $1,200 MM. Thus potential savings of $200 MM to $600 MM are possible if drilling rates are doubled [assuming bit life is reasonable]. The net result for operators is improved profit margin as well as an improved position on reserves. The significance of the ''ultra-high rotary speed drilling system'' is the ability to drill into rock at very low weights on bit and possibly lower energy levels. The drilling and coring industry today does not practice this technology. The highest rotary speed systems in oil field and mining drilling and coring today run less than 10,000 rpm--usually well below 5,000 rpm. This document details the progress to date on the program entitled ''SMALLER FOOTPRINT DRILLING SYSTEM FOR DEEP AND HARD ROCK ENVIRONMENTS; FEASIBILITY OF ULTRA-HIGH SPEED DIAMOND DRILLING'' for the period starting June 23, 2003 through September 30, 2004. (1) TerraTek has reviewed applicable literature and documentation and has convened a project kick-off meeting with Industry Advisors in attendance. (2) TerraTek has designed and planned Phase I bench scale experiments. Some difficulties in obtaining ultra-high speed motors for this feasibility work were encountered though they were sourced mid 2004. (3) TerraTek is progressing through Task 3 ''Small-scale cutting performance tests''. Some improvements over early NASA experiments have been identified.« less

  18. ICDP drilling in the Scandinavian Caledonides: the SDDP-COSC project

    NASA Astrophysics Data System (ADS)

    Lorenz, Henning; Juhlin, Christopher; Gee, David; Pascal, Christophe; Tsang, Chin-Fu; Pedersen, Karsten; Rosberg, Jan-Erik

    2013-04-01

    The Swedish Deep Drilling Program (SDDP) Collisional Orogeny in the Scandinavian Caledonides (COSC) project is a multidisciplinary investigation of the Scandian mountain belt. Cenozoic uplift of the Scandes has exposed a lower- to middle-crustal level section through this Himalaya-type orogen, providing unique opportunities to better understand not only the Caledonides, but also on-going orogeny and the earthquake-prone environments of modern mountains belts. COSC will also contribute to our knowledge of mountain belt hydrology, provide the first information about deep thermal gradients for paleoclimate modeling and potential geothermal energy resources, contribute new information about the deep biosphere, and improve our understanding of the Cenozoic uplift history of the Scandes. The drilling program targets the far-traveled (> 400 km) allochthons of the Scandinavian Caledonides and their emplacement across the Baltoscandian foreland basin onto the platform of continent Baltica. Two 2.5 km deep holes are planned. COSC-1, to be drilled in the summer of 2013, will target the high-grade metamorphic complex of the Seve Nappes (SNC) and its contact to underlying allochthons. COSC-2 will start in the lower thrust sheets, pass through the basal décollement and investigate the character of the deformation in the underlying basement. An international science team, including expertise on Himalaya-Tibet and other young orogens, is running the science program. New high-resolution reflection seismic data provide excellent images of the upper crust. Alternative interpretations of the reflectors' origin, particularly those in the basement, will be tested. The site of COSC-1 is based on a 3D geological model, constructed from surface geology, recent and vintage regional reflection seismic profiles, regional and local gravity data, and high-resolution aeromagnetics, acquired recently by the Geological Survey of Sweden. The drilling will be carried out utilising the new Swedish scientific drilling infrastructure, located at Lund University, an Atlas Copco CT20 diamond core-drilling rig, with versatile drilling equipment (see EGU2012-7379), providing the ideal platform for core-drilling to 2.5 km depths. Existing drilling, sampling and testing techniques (e.g. triple-tube core drilling for best core quality) will need to be adapted to highly variable lithologies and new techniques will be developed, as necessary. COSC-1 drilling operations and the directly related on-site investigations are financed by ICDP and the Swedish Research Council. All drill cores will be transferred to the core repository of the Geological Survey of Sweden, and a sampling party will be announced later this year. Researchers who want to participate in COSC and contribute their expertise are encouraged to inform us of their interests.

  19. Initial report on drilling into seismogenic zones of M2.0 - M5.5 earthquakes from deep South African gold mines (DSeis)

    NASA Astrophysics Data System (ADS)

    Ogasawara, Hiroshi; Durrheim, Raymond; Yabe, Yasuo; Ito, Takatoshi; van Aswegen, Gerrie; Grobbelaar, Michelle; Funato, Akio; Ishida, Akimasa; Ogasawara, Hiroyuki; Mngadi, Siyanda; Manzi, Musa; Ziegler, Martin; Ward, Tony; Moyer, Pamela; Boettcher, Margaret; Ellsworth, Bill; Liebenberg, Bennie; Wechsler, Neta; Onstott, Tullis; Berset, Nicolas

    2017-04-01

    The International Continental Scientific Drilling Program (ICDP) approved our proposal (Ogasawara et al., EGU 2016) to drill into and around seismogenic zones where critically stressed faults initiated ruptures at depth. The drilling targets include four ruptures equivalent to M2.0, 2.8, 3.5, and 5.5 that dynamically and quasi-statically evolved in 2.9 Ga hard rock in the Witwatersrand basin, South Africa. Major advantages of our drilling locations are the large quantity and high-quality of existing data from dense seismic arrays both on surface and near-field underground in three deep South African gold mines. Additionally, the great depths (1.0 to 3.3 km from surface) to collar holes reduce drilling costs significantly and enable a larger number of holes to be drilled. Flexibility in drilling direction will also allow us to minimize damage in borehole or drilled cores. With the ICDP funds, we will conduct full-core drilling of 16 holes with drilling ranges from 50 to 750 m to recover both materials and fractures in and around the seismogenic zones, followed by core and borehole logging. Additional in-hole monitoring at close proximity will be supported by co-mingled funds and will follow the ICDP drilling. Expected magnitudes of maximum shear stress are several tens of MPa. We have established an overcoring procedure to measure 3D-stress state for adverse underground working conditions so as not to interfere with mining operations. This procedure was optimized based on the Compact Conic-ended Borehole Overcoring (CCBO) technique (ISRM suggested; Sugawara and Obara, 1999). Funato and Ito (2016 IJRMMS) developed a diametrical core deformation analysis (DCDA) method to measure differential stress using only drilled core by assuming diametrical change with roll angles caused by elastic in-axisymmetrical expansion during drilling. A gold mine has already drilled a hole to intersect the hypocenter of a 2016 M3.5 earthquake and carried out the CCBO stress measurement in other holes at the M3.5 seismogenic zone. As we successfully conducted DCDA with the above-mentioned drilled core, we look forward to shedding light on spatial variations of stress in the seismogenic zones following our ICDP DSeis drilling. A M5.5 earthquake which took place near Orkney, South Africa on 5 August 2014, offers a special opportunity to compare seismically inverted spatio-temporal evolution of both the main rupture and the aftershock activity with the information directly probed by the ICDP DSeis project. Moyer et al. (2016 Seismol. Res. Lett. submitted) calls for comparing seismic source models as part of a workshop proposed to the Southern California Earthquake Center for Fall 2017. In addition, the upper edge of the M5.5 rupture is located hundreds of meters below the mining horizon, sufficiently away from anthropogenic activity. This allows geomicrobiologists to investigate deep microbiological activity fueled by H2 from seismic rupture to address questions about Earth's early life. Drilling machines are being rigged underground soon to kick off our ICDP DSeis drilling in early 2017.

  20. Electronic Nose

    NASA Technical Reports Server (NTRS)

    1982-01-01

    Grace Industries, Inc.'s Electronic Nose is a vapor and gas detector, deriving from NASA's electronic circuitry, capable for sensing the presence of accelerants several days after a fire. The device is powered by rechargeable battery and no special training needed to operate. If an accelerant is present, device will emit a beeping sound and trigger a flashing light; the faster the beep rate, the more volatile the accelerant. Its sensitivity can also detect minute traces of accelerants. Unit saves investigators of fire causes time and expense by providing speedy detection of physical evidence for use in court. Device is also useful for detecting hazardous fumes, locating and detecting gas leaks in refineries and on oil drilling rigs.

  1. A hybrid floating brush seal (HFBS) for improved sealing and wear performance in turbomachinery applications

    NASA Astrophysics Data System (ADS)

    Lattime, Scott Byran

    A conceptually new type of seal has been developed for gas turbine applications which dramatically reduces wear and leakage associated with current labyrinth and brush seal technologies. The Hybrid Floating Brush Seal (HFBS) combines brush seal and film riding face seal technologies to create a hybrid seal that allows both axial and radial excursions of the sealed shaft, while simultaneously eliminating interface surface speeds (friction and heat) between the rotor and the brush material that characterize standard brush seal technology. A simple test rig was designed to evaluate feasibility of the HFBS under relatively low pressures and rotational speeds (50psig, 5krpm). A second test stand was created to study the effects of centrifugal force on bristle deflection. A third test facility was constructed for prototype development and extensive room temperature testing at moderate pressures and fairly high rotational speeds (100psig, 40krpm). This test rig also allowed the evaluation of the HFBS during axial movement of a rotating shaft. An analytical model to predict the effects of centrifugal force on the bristles of a rotating brush seal was developed. Room temperature analysis of the HFBS proved successful for relatively high operating rotational velocities at moderate pressures with very acceptable leakage rates for gas turbine engines. Brush seals were able to track rotor speeds up to 24krpm while maintaining sealing integrity. The HFBS's ability to function under axial shaft displacement and synchronous dynamic radial loading was also proven successful. Hydrodynamic performance of the face seal was proven to provide adequate stiffness and load carrying capacity to keep the brush seal from contacting the face seal at pressure drops across the brush of up to 100psi. Leakage performance over standard brush seal and labyrinth technology was quite dramatic. The HFBS showed its sealing advantage using much higher radial interference between the rotor and the bristle bore over standard brush seal designs. Experimental results of the HFBS showed leakage reductions of 6 to 7 times that of a standard brush seal at the same operating pressure ratios and rotational speed and an order of magnitude less than numerical predictions of a standard labyrinth seal. (Abstract shortened by UMI.)

  2. Use of Cutting-Edge Horizontal and Underbalanced Drilling Technologies and Subsurface Seismic Techniques to Explore, Drill and Produce Reservoired Oil and Gas from the Fractured Monterey Below 10,000 ft in the Santa Maria Basin of California

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    George Witter; Robert Knoll; William Rehm

    2006-06-30

    This project was undertaken to demonstrate that oil and gas can be drilled and produced safely and economically from a fractured Monterey reservoir in the Santa Maria Basin of California by employing horizontal wellbores and underbalanced drilling technologies. Two vertical wells were previously drilled in this area with heavy mud and conventional completions; neither was commercially productive. A new well was drilled by the project team in 2004 with the objective of accessing an extended length of oil-bearing, high-resistivity Monterey shale via a horizontal wellbore, while implementing managed-pressure drilling (MPD) techniques to avoid formation damage. Initial project meetings were conductedmore » in October 2003. The team confirmed that the demonstration well would be completed open-hole to minimize productivity impairment. Following an overview of the geologic setting and local field experience, critical aspects of the application were identified. At the pre-spud meeting in January 2004, the final well design was confirmed and the well programming/service company requirements assigned. Various design elements were reduced in scope due to significant budgetary constraints. Major alterations to the original plan included: (1) a VSP seismic survey was delayed to a later phase; (2) a new (larger) surface hole would be drilled rather than re-enter an existing well; (3) a 7-in. liner would be placed into the top of the Monterey target as quickly as possible to avoid problems with hole stability; (4) evaluation activities were reduced in scope; (5) geosteering observations for fracture access would be deduced from penetration rate, cuttings description and hydrocarbon in-flow; and (6) rather than use nitrogen, a novel air-injection MPD system was to be implemented. Drilling operations, delayed from the original schedule by capital constraints and lack of rig availability, were conducted from September 12 to November 11, 2004. The vertical and upper curved sections were drilled and lined through the problematic shale member without major stability problems. The top of the targeted Monterey was thought to be seen at the expected TVD of 10,000 ft where the 7-in. liner was set at a 60{sup o} hole angle. Significant oil and gas shows suggested the fractured interval anticipated at the heel location had been penetrated. A total of 2572 ft of 6{Delta}-in. near-horizontal interval was placed in the shale section, extending planned well length by approximately 470 ft. Very little hydrocarbon in-flow was observed from fractures along the productive interval. This may be a result of the well trajectory falling underneath the Monterey fractured zone. Hydrocarbon observations, cuttings analysis and gamma-ray response indicated additional fractured intervals were accessed along the last {+-}900 ft of well length. The well was completed with a 2 and 7/8-in. tubing string set in a production packer in preparation for flow and swab tests to be conducted later by a service rig. The planned well time was estimated as 39 days and overall cost as $2.4 million. The actual results are 66 days at a total cost of $3.4 million. Well productivity responses during subsequent flow and swabbing tests were negative. The well failed to inflow and only minor amounts (a few barrels) of light oil were recovered. The lack of production may suggest that actual sustainable reservoir pressure is far less than anticipated. Temblor attempted in July, 2006, to re-enter and clean out the well and run an Array Induction log (primarily for resistivity and correlation purposes), and an FMI log (for fracture detection). Application of surfactant in the length of the horizontal hole, and acid over the fracture zone at 10,236 was also planned. This attempt was not successful in that the clean out tools became stuck and had to be abandoned.« less

  3. Simple, affordable and sustainable borehole observatories for complex monitoring objectives

    NASA Astrophysics Data System (ADS)

    Kopf, A.; Freudenthal, T.; Ratmeyer, V.; Bergenthal, M.; Lange, M.; Fleischmann, T.; Hammerschmidt, S.; Seiter, C.; Wefer, G.

    2014-12-01

    Seafloor drill rigs are remotely operated systems that provide a cost effective means to recover sedimentary records of the upper sub-seafloor deposits. Recent increases in their payload included downhole logging tools or autoclave coring systems. We here report on another milestone in using seafloor rigs: the development and installation of shallow borehole observatories. Three different systems have been developed for the MARUM-MeBo seafloor drill, which is operated by MARUM, University of Bremen, Germany. A simple design, the MeBoPLUG, separates the inner borehole from the overlying ocean by using o-ring seals at the conical threads of the drill pipe. The systems are self-contained and include data loggers, batteries, thermistors and a differential pressure sensor. A second design, the so-called MeBoCORK, is more sophisticated and also hosts an acoustic modem for data transfer and, if desired, fluid sampling capability using osmotic pumps. Of these MeBoCORKs, two systems have to be distinguished: the CORK-A (A = autonomous) can be installed by the MeBo alone and monitors pressure and temperature inside and above the borehole (the latter for reference). The CORK-B (B = bottom) has a higher payload and can additionally be equipped with geochemical, biological or other physical components. Owing to its larger size, it is installed by ROV and utilises a hotstab connection in the upper portion of the drill string. Either design relies on a hotstab connection from beneath which coiled tubing with a conical drop weight is lowered to couple to the formation. These tubes are fluid-saturated and either serve to transmit pore pressure signals or collect pore water in the osmo-sampler. The third design, the MeBoPUPPI (Pop-Up Pore Pressure Instrument), is similar to the MeBoCORK-A and monitors pore pressure and temperature in a self-contained manner. Instead of transferring data upon command using an acoustic modem, the MeBoPUPPI contains a pop-up telemetry with Iridium link. After a predefined period, the data unit with satellite link is released, ascends to the sea surface, and remains there for up to two weeks while sending the long-term data sets to shore. In summer 2012, two MeBoPLUGs, one MeBoCORK-A and one MeBoCORK-B were installed with MeBo on German RV Sonne in the Nankai Trough area, Japan. We have successfully downloaded data from the CORKs, attesting that coupling to the formation worked and pressure records were elevated relative to the seafloor reference. In the near future, we will further deploy the first two MeBoPUPPIs. Recovery of all monitoring systems by ROV is planned for 2016.

  4. Issues in offshore platform research - Part 1: Semi-submersibles

    NASA Astrophysics Data System (ADS)

    Sharma, R.; Kim, Tae-Wan; Sha, O. P.; Misra, S. C.

    2010-09-01

    Availability of economic and efficient energy resources is crucial to a nation's development. Because of their low cost and advancement in drilling and exploration technologies, oil and gas based energy systems are the most widely used energy source throughout the world. The inexpensive oil and gas based energy systems are used for everything, i.e., from transportation of goods and people to the harvesting of crops for food. As the energy demand continues to rise, there is strong need for inexpensive energy solutions. An offshore platform is a large structure that is used to house workers and machinery needed to drill wells in the ocean bed, extract oil and/or natural gas, process the produced fluids, and ship or pipe them to shore. Depending on the circumstances, the offshore platform can be fixed (to the ocean floor) or can consist of an artificial island or can float. Semi-submersibles are used for various purposes in offshore and marine engineering, e.g. crane vessels, drilling vessels, tourist vessels, production platforms and accommodation facilities, etc. The challenges of deepwater drilling have further motivated the researchers to design optimum choices for semi-submersibles for a chosen operating depth. In our series of eight papers, we discuss the design and production aspects of all the types of offshore platforms. In the present part I, we present an introduction and critical analysis of semi-submersibles.

  5. Deep-sea observations at hydrocarbon drilling locations: Contributions from the SERPENT Project after 120 field visits

    NASA Astrophysics Data System (ADS)

    Gates, Andrew R.; Benfield, Mark C.; Booth, David J.; Fowler, Ashley M.; Skropeta, Danielle; Jones, Daniel O. B.

    2017-03-01

    The SERPENT Project has been running for over ten years. In this time scientists from universities and research institutions have made more than 120 visits to oil rigs, drill ships and survey vessels operated by 16 oil companies, in order to work with the industry's Remotely Operated Vehicles (ROV). Visits have taken place in Europe, North and South America, Africa and Australasia at water depths from 100 m to nearly 3000 m. The project has directly produced >40 peer reviewed publications and data from the project's >2600 entry online image and video archive have been used in many others. The aim of this paper is to highlight examples of how valuable data can be obtained through collaboration with hydrocarbon exploration and production companies to use existing industry infrastructure to increase scientific discovery in unexplored areas and augment environmental monitoring of industrial activity. The large number of industry ROVs operating globally increases chance encounters with large, enigmatic marine organisms. SERPENT video observations include the deepest known records of species previously considered epipelagic such as scalloped hammerhead (Sphyrna lewini) and southern sunfish (Mola ramsayi) and the first in situ observations of pelagic species such as oarfish (Regalecus glesne). Such observations enable improvements to distribution records and description of behaviour of poorly understood species. Specimen collection has been used for taxonomic descriptions, functional studies and natural products chemistry research. Anthropogenic effects been assessed at the local scale using in situ observations and sample collection at the time of drilling operations and subsequent visits have enabled study of recovery from drilling. Future challenges to be addressed using the SERPENT approach include ensuring unique faunal observations by industry ROV operators are reported, further study of recovery from deep-water drilling activity and to carry out in situ studies to improve the understanding of potential future decommissioning of obsolete hydrocarbon infrastructure.

  6. In Situ Temperature Measurements at the Svalbard Continental Margin: Implications for Gas Hydrate Dynamics

    NASA Astrophysics Data System (ADS)

    Riedel, M.; Wallmann, K.; Berndt, C.; Pape, T.; Freudenthal, T.; Bergenthal, M.; Bünz, S.; Bohrmann, G.

    2018-04-01

    During expedition MARIA S. MERIAN MSM57/2 to the Svalbard margin offshore Prins Karls Forland, the seafloor drill rig MARUM-MeBo70 was used to assess the landward termination of the gas hydrate system in water depths between 340 and 446 m. The study region shows abundant seafloor gas vents, clustered at a water depth of ˜400 m. The sedimentary environment within the upper 100 m below seafloor (mbsf) is dominated by ice-berg scours and glacial unconformities. Sediments cored included glacial diamictons and sheet-sands interbedded with mud. Seismic data show a bottom simulating reflector terminating ˜30 km seaward in ˜760 m water depth before it reaches the theoretical limit of the gas hydrate stability zone (GHSZ) at the drilling transect. We present results of the first in situ temperature measurements conducted with MeBo70 down to 28 mbsf. The data yield temperature gradients between ˜38°C km-1 at the deepest site (446 m) and ˜41°C km-1 at a shallower drill site (390 m). These data constrain combined with in situ pore-fluid data, sediment porosities, and thermal conductivities the dynamic evolution of the GHSZ during the past 70 years for which bottom water temperature records exist. Gas hydrate is not stable in the sediments at sites shallower than 390 m water depth at the time of acquisition (August 2016). Only at the drill site in 446 m water depth, favorable gas hydrate stability conditions are met (maximum vertical extent of ˜60 mbsf); however, coring did not encounter any gas hydrates.

  7. Assessment of Methane Emissions – Impact of Using Natural Gas Engines in Unconventional Resource Development

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Nix, Andrew; Johnson, Derek; Heltzel, Robert

    Researchers at the Center for Alternative Fuels, Engines, and Emissions (CAFEE) completed a multi-year program under DE-FE0013689 entitled, “Assessing Fugitive Methane Emissions Impact Using Natural Gas Engines in Unconventional Resource Development.” When drilling activity was high and industry sought to lower operating costs and reduce emissions they began investing in dual fuel and dedicated natural gas engines to power unconventional well equipment. From a review of literature we determined that the prime-movers (or major fuel consumers) of unconventional well development were the service trucks (trucking), horizontal drilling rig (drilling) engines, and hydraulic stimulation pump (fracturing) engines. Based on early findingsmore » from on-road studies we assessed that conversion of prime movers to operate on natural gas could contribute to methane emissions associated with unconventional wells. As such, we collected significant in-use activity data from service trucks and in-use activity, fuel consumption, and gaseous emissions data from drilling and fracturing engines. Our findings confirmed that conversion of the prime movers to operate as dual fuel or dedicated natural gas – created an additional source of methane emissions. While some gaseous emissions were decreased from implementation of these technologies – methane and CO 2 equivalent emissions tended to increase, especially for non-road engines. The increases were highest for dual fuel engines due to methane slip from the exhaust and engine crankcase. Dedicated natural gas engines tended to have lower exhaust methane emissions but higher CO 2 emissions due to lower efficiency. Therefore, investing in currently available natural gas technologies for prime movers will increase the greenhouse gas footprint of the unconventional well development industry.« less

  8. Punched belt hole position deviation analysis of float type water level gauge

    NASA Astrophysics Data System (ADS)

    Mao, Chunlei; Wang, Tao; Fu, Weijie; Li, Lianhui

    2018-03-01

    The key parts of the float type water level gauge instrument is perforated belt, The size and tolerance requirements of its aperture is: (1) alternation of 100+0.2 and 100-0.2, (2) 200±0.1, (3) 1000±0.15, (4) 10000±0.2. The single hole position: alternation of 100+0.2 and 100-0.2; double: 200±0.1, and ensure the best hole position error avoidance tends to be one-way, that is to say: when the punched belt combined with a water wheel rotating line moving, The hole position error to single direction increase or decrease, caused the water level nail gradually and close to the edge of the hole, and then edge and final punched belt was lifted. This paper uses the laser drilling process of steel strip for data collection and analysis. It is found that this method cannot meet the tolerance requirements and the double stamping processing method with adjustable cylindrical pin is feasible.

  9. [Occupational morbidity among miners engaged into contemporary method of extracting copper-nickel ores in Kola Transpolar regions].

    PubMed

    Siurin, S A; Shilov, V V

    2014-01-01

    The risks for developing occupational pathology and its specific features were studied in 358 Kola Transpolar copper-nickel miners who were diagnosed with 722 cases of occupational diseases (OD) in the years 1990-2013. The highest risk for developing OD, which are dominated by the diseases of the musculoskeletal system, has been found in tunnellers (OR = 12,8) and operators of drilling rigs (OR = 10,4). A significant increase in the risk of OD has been established in miners with length of service at 11-15 years and over 25 years. The conclusion is made about the need to improve the technical, medical and organizational measures targetted at preventing health problems in this group of workers.

  10. Seismic Stability Evaluation of Folsom Dam and Reservoir Project. Report 4. Mormon Island Auxiliary Dam. Phase 1

    DTIC Science & Technology

    1990-12-01

    soundings were made with a Felcon bit which is a 3- web crowd-in bit for 6-5/8-in. casing but has an enlarged diameter near the bit (7-1/4-in. OD) and...Fed Figure 40. Becker Htammer drilling rig Figure 41. Three- web crowd-in Felcon bit for open-bit penetration testing and sampling, and eight-tooth crowd...0 000 l’ U 900 o US U 0 m wIX = r = 0 c 1 ,- 2 cc w > uW~- 0 .4-t)t UO: >. N m Cj U 0 (I uuja:o o o oH 00 U~~ u C1 to F-4 m 0 0 V) V) 0) NN 00_

  11. Application of MRIL-WD (Magnetic Resonance Imaging Logging While Drilling) for irreducible water saturation, total reservoir, free-fluid, bound-fluid porosity measurements and its value for the petrophysical analysis of RT/RM data from the Shah Deniz well

    NASA Astrophysics Data System (ADS)

    Amirov, Elnur

    2016-04-01

    Sperry-Sun (Sperry Drilling Services) is the leader in MWD/LWD reliability, has developed the industry's first LWD NMR/MRIL-WD (nuclear magnetic resonance) tool. The MRIL-WD (magnetic resonance imaging logging-while-drilling) service directly measures the T1 component of hydrogen in subsurface rock units while drilling to obtain total reservoir porosity and to dissect the observed total porosity into its respective components of free fluid and bound fluid porosity. These T1 data are used to secure accurate total, free-fluid, capillary-bound water, and clay-bound water porosity of the reservoir sections which can be drilled in the several Runs. Over the last decade, results from Magnetic Resonance Imaging logs (NMR) have added significant value to petrophysical analysis and understanding by providing total, free-fluid and bound-fluid porosities, combined with fluid typing capabilities. With MRIL-WD very valuable Real-Time or Recorded Memory data/information is now available during or shortly after the drilling operation (formation properties measurement can be taken right after a drill bit penetration), while trip in and trip out as well. A key point in utilizing MRIL in an LWD environment is motion-tolerant measurements. Recent MRIL-WD logging runs from the Shah Deniz wells located in the Khazarian-Caspian Sea of the Azerbaijan Republic helped to delineate and assess hydrocarbon bearing zones. Acquired results demonstrate how MRIL data can be acquired while-drilling and provide reliable/high quality measurements. Magnetic Resonance Imaging logs at some developments wells have become a cornerstone in formation evaluation and petrophysical understanding. By providing total, free-fluid, and bound-fluid porosities together with fluid typing, MRIL results have significantly added to the assessment of reservoirs. In order to reduce NPT (Non-Productive Time) and save the rig operations time, there is always the desire to obtain logging results as soon as possible, preferably while the drilling of the brand new wells (logging-while-drilling, LWD). The MRIL-WD Tool can accomplish any tasks reliably and in a timely manner thus saving drilling time and reducing the overall risk for the well. Control of water production and identification of pay zones with high irreducible water saturation are also very important for formation evaluation and petrophysical analysis in oil fields located in the Azerbaijan Republic and also other fields around the world. Sometimes above-mentioned problems can cause delay in completion decisions which will create additional expenses for field management. In many wells, breakthroughs in reservoir characterization have been achieved in directly determining hydrocarbon volumes, net permeability thickness, and hydrocarbon type, thus circumventing the problems associated with obtaining wireline data and the considerable amount of rig time required (so MRIL-WD can considerably reduce the NPT). Some reservoir zones with relatively low water saturation, which calculated from the other conventional logs, can produce with relatively high percentage of water cut, primarily because much of the water is movable. However, other zones with high calculated water saturation produce water free hydrocarbons. The difficulty in predicting water production can be related with the producing from the complex lithology, which can contain low-permeability, medium- to fine-grained shaly sands. Where grains are small, the formations have high surface to volume ratios that result in high irreducible water saturation and due to this we can see low resistivity values. As a result the use of resistivity logs as pay indicator, sometimes can cause low resistivity pay zones might be overlooked and consequently net field pay could be underestimated. In the last few years, nuclear magnetic resonance logs have shown great promise in solving problems of formation evaluation that could not be directly resolved with conventional logs. The capability of MRIL-WD can help many engineers to differentiate between the immovable and movable water in oil reservoirs in many fields. Sometimes MRIL-WD have also been capable of providing better formation permeability than conventional logs, a feature which can save time and expense in well-completion decisions. The RT & RM bound fluid and total porosity measurements can provide a tremendous new insight into the formation evaluation of shaly sands and low resistivity pays. Unlike traditional porosity devices, which are affected by rock matrix changes, the MRIL-WD tool can be used in complex or mixed lithology sequences and provide measurements of porosity that are lithology independent.

  12. Smaller Footprint Drilling System for Deep and Hard Rock Environments; Feasibility of Ultra-High-Speed Diamond Drilling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Arnis Judzis; Homer Robertson; Alan Black

    2006-06-22

    The two phase program addresses long-term developments in deep well and hard rock drilling. TerraTek believes that significant improvements in drilling deep hard rock will be obtained by applying ultra-high rotational speeds (greater than 10,000 rpm). The work includes a feasibility of concept research effort aimed at development that will ultimately result in the ability to reliably drill ''faster and deeper'' possibly with smaller, more mobile rigs. The principle focus is on demonstration testing of diamond bits rotating at speeds in excess of 10,000 rpm to achieve high rate of penetration (ROP) rock cutting with substantially lower inputs of energymore » and loads. The significance of the ''ultra-high rotary speed drilling system'' is the ability to drill into rock at very low weights on bit and possibly lower energy levels. The drilling and coring industry today does not practice this technology. The highest rotary speed systems in oil field and mining drilling and coring today run less than 10,000 rpm-usually well below 5,000 rpm. This document details the progress at the end of Phase 1 on the program entitled ''Smaller Footprint Drilling System for Deep and Hard Rock Environments: Feasibility of Ultra-High-Speed Diamond Drilling'' for the period starting 1 March 2006 and concluding 30 June 2006. (Note: Results from 1 September 2005 through 28 February 2006 were included in the previous report (see Judzis, Black, and Robertson)). Summarizing the accomplished during Phase 1: {lg_bullet} TerraTek reviewed applicable literature and documentation and convened a project kickoff meeting with Industry Advisors in attendance (see Black and Judzis). {lg_bullet} TerraTek designed and planned Phase I bench scale experiments (See Black and Judzis). Some difficulties continued in obtaining ultra-high speed motors. Improvements were made to the loading mechanism and the rotational speed monitoring instrumentation. New drill bit designs were developed to provided a more consistent product with consistent performance. A test matrix for the final core bit testing program was completed. {lg_bullet} TerraTek concluded Task 3 ''Small-scale cutting performance tests.'' {sm_bullet} Significant testing was performed on nine different rocks. {sm_bullet} Five rocks were used for the final testing. The final tests were based on statistical design of experiments. {sm_bullet} Two full-faced bits, a small diameter and a large diameter, were run in Berea sandstone. {lg_bullet} Analysis of data was completed and indicates that there is decreased specific energy as the rotational speed increases (Task 4). Data analysis from early trials was used to direct the efforts of the final testing for Phase I (Task 5). {lg_bullet} Technology transfer (Task 6) was accomplished with technical presentations to the industry (see Judzis, Boucher, McCammon, and Black).« less

  13. Dynamic behavior and deformation analysis of the fish cage system using mass-spring model

    NASA Astrophysics Data System (ADS)

    Lee, Chun Woo; Lee, Jihoon; Park, Subong

    2015-06-01

    Fish cage systems are influenced by various oceanic conditions, and the movements and deformation of the system by the external forces can affect the safety of the system itself, as well as the species of fish being cultivated. Structural durability of the system against environmental factors has been major concern for the marine aquaculture system. In this research, a mathematical model and a simulation method were presented for analyzing the performance of the large-scale fish cage system influenced by current and waves. The cage system consisted of netting, mooring ropes, floats, sinkers and floating collar. All the elements were modeled by use of the mass-spring model. The structures were divided into finite elements and mass points were placed at the mid-point of each element, and mass points were connected by springs without mass. Each mass point was applied to external and internal forces, and total force was calculated in every integration step. The computation method was applied to the dynamic simulation of the actual fish cage systems rigged with synthetic fiber and copper wire simultaneously influenced by current and waves. Here, we also tried to find a relevant ratio between buoyancy and sinking force of the fish cages. The simulation results provide improved understanding of the behavior of the structure and valuable information concerning optimum ratio of the buoyancy to sinking force according to current speeds.

  14. Seal Technology for Liquid Oxygen (LOX) Turbopumps

    NASA Technical Reports Server (NTRS)

    Shapiro, Wilbur; Hamm, Robert

    1985-01-01

    Two types of advanced seals for liquid oxygen (LOX) turbopumps were investigated. One was a spiral-groove face seal whose function is to seal high-pressure LOX at the impeller end of the turbopump. The other was a floating-ring, Rayleigh-step, helium buffered seal used to prevent LOX ingress to the turbine side of the unit. For each seal type, two sizes were investigated (50 and 20 mm). A turbine-driven test rig was designed and manufactured, and a test program was completed on the 50 mm floating-ring, Rayleigh-step, helium buffered seal. Significant results were: vaporization in the flow path could cause failure by overheating; therefore, the spiral-groove pumping portion of the seal that provides the fluid film must circulate fluid without disruption if vaporization occurs in the sealing dam. This is successfully accomplished by a pressure-balanced spiral-groove concept that is described. The spiral-groove configuration is affected by turbulence in the fluid film and pressure drops due to fluid inertia at sudden contractions. The net results of these effects are deep grooves, large operating films, and high power loss when compared against seals operating with laminar films. Turbulence and inertia are induced by the high-density and low-viscosity characteristics of LOX. The program clearly pointed out the need to consider system environmental factors such as thermal and centrifugal distortions and rotor vibrations in the seal design.

  15. Shipping lanes or offshore rigs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1980-09-01

    This information was from the Los Angeles Steamship Association (LASSA) luncheon meeting. The problems of limiting access and availability of the Santa Barbara/Santa Catalina channels to commercial vessel traffic and other related uses. LASSA speaks for about 85% of the maritime industry in Southern California. The Association is actively seeking a compromise with the oil companies in keeping the Vessel Traffic Separation Scheme (VTSS) in the channels; however, the Western Oil and Gas Association (WOGA) is seeking to abolish VTSS as currently established in the channels and move the sea lanes outside the Channel Islands, and open up the entiremore » Santa Barbara Channel to unlimited drilling sites. LASSA claims that moving the VTSS sea lanes outside of the Channel Islands would add 18 to 22 miles to the average trip from San Francisco to Los Angeles, with fuel cost etc. would make for a big loss to the merchant ship operators. LASSA has offered to support the concept of opening up the Buffer Zone that separates the Sea Lanes themselves to exploratory drilling. This two mile wide stretch of water is off limits to vessels and it would open new areas to the oil companies heretofore unaccessible to them. (DP)« less

  16. Method for rigless zone abandonment using internally catalyzed resin system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Martin, R.C.

    1980-02-19

    A zone of a subterranean formation penetrated by a well bore is permanently plugged by injecting a liquid resin system containing at least one thermosetting resin and at least one curing agent or catalyst therefor into the formation and injecting into the wellbore following the resin system, a second liquid containing at least one chain stopping compound to react with one component in the resin system to prevent any of the resin system remaining in the well bore from crosslinking to a sufficient crosslink density to form a solid in the wellbore. Preferably, the second liquid also contains a fluidmore » loss additive to minimize loss of the second liquid from the wellbore to the formation. The method permits a zone to be plugged off and abandoned without the need to erect a drilling rig to drill out excess plugging material remaining in the wellbore. In a preferred embodiment, the resin system comprises the diglycidyl ether of bisphenol a and polymethylene phenylamine in ethylene glycol ethyl ether, and the preferred second liquid is monoethanolamine in ethylene glycol ethyl ether as a solvent with ethylcellulose and silic flour to control fluid loss.« less

  17. Composition for preventing a resin system from setting up in a well bore

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Martin, R. C.

    1981-06-09

    A zone of a subterranean formation penetrated by a well bore is permanently plugged by injecting a liquid resin system containing at least one thermosetting resin and at least one curing agent or catalyst therefor into the formation and injecting into the wellbore following the resin system, a second liquid containing at least one chain stopping compound to react with one component in the resin system to prevent any of the resin system remaining in the well bore from crosslinking to a sufficient crosslink density to form a solid in the wellbore. Preferably, the second liquid also contains a fluidmore » loss additive to minimize loss of the second liquid from the wellbore to the formation. The method permits a zone to be plugged off and abandoned without the need to erect a drilling rig to drill out excess plugging material remaining in the wellbore. In a preferred embodiment, the resin system comprises the diglycidyl ether of bisphenol a and polymethylene phenylamine in ethylene glycol ethyl ether, and the preferred second liquid is monoethanolamine in ethylene glycol ethyl ether as a solvent with ethylcellulose and silica flour to control fluid loss.« less

  18. New mud system produces solids-free, reusable water

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-02-01

    The Corpus Christi, Texas, based Cameron Equipment Co., Inc., has developed a closed-loop mud treating system that removes solids from water-based systems and leaves the separated fluid clean and chemical free enough to be re-used directly on the rig. The system has been successfully applied by a Gulf of Mexico operator in areas where zero discharge is required. The alternative mud conditions program offered by the developers is called the Cameron Fluid Recycling System. Designed for closed-loop water-based fluids, the system is a new method of removing solids from normally discharged fluids such as drilling mud, waste and wash water,more » or any other water-based fluid that contains undesirable solids. The patented method efficiently produces end products that are (1) dry solids; and (2) essentially 100% solids-free fluid that can be re-used in the same mud system. All excess drilling mud, and all wash water that would normally go to the reserve pit or a cuttings barge are collected in a tank. Recycled fluid is compatible with the mud system fluid, no harmful chemicals are used, and pH is not altered.« less

  19. Classification scheme for sedimentary and igneous rocks in Gale crater, Mars

    NASA Astrophysics Data System (ADS)

    Mangold, N.; Schmidt, M. E.; Fisk, M. R.; Forni, O.; McLennan, S. M.; Ming, D. W.; Sautter, V.; Sumner, D.; Williams, A. J.; Clegg, S. M.; Cousin, A.; Gasnault, O.; Gellert, R.; Grotzinger, J. P.; Wiens, R. C.

    2017-03-01

    Rocks analyzed by the Curiosity rover in Gale crater include a variety of clastic sedimentary rocks and igneous float rocks transported by fluvial and impact processes. To facilitate the discussion of the range of lithologies, we present in this article a petrological classification framework adapting terrestrial classification schemes to Mars compositions (such as Fe abundances typically higher than for comparable lithologies on Earth), to specific Curiosity observations (such as common alkali-rich rocks), and to the capabilities of the rover instruments. Mineralogy was acquired only locally for a few drilled rocks, and so it does not suffice as a systematic classification tool, in contrast to classical terrestrial rock classification. The core of this classification involves (1) the characterization of rock texture as sedimentary, igneous or undefined according to grain/crystal sizes and shapes using imaging from the ChemCam Remote Micro-Imager (RMI), Mars Hand Lens Imager (MAHLI) and Mastcam instruments, and (2) the assignment of geochemical modifiers based on the abundances of Fe, Si, alkali, and S determined by the Alpha Particle X-ray Spectrometer (APXS) and ChemCam instruments. The aims are to help understand Gale crater geology by highlighting the various categories of rocks analyzed by the rover. Several implications are proposed from the cross-comparisons of rocks of various texture and composition, for instance between in place outcrops and float rocks. All outcrops analyzed by the rover are sedimentary; no igneous outcrops have been observed. However, some igneous rocks are clasts in conglomerates, suggesting that part of them are derived from the crater rim. The compositions of in-place sedimentary rocks contrast significantly with the compositions of igneous float rocks. While some of the differences between sedimentary rocks and igneous floats may be related to physical sorting and diagenesis of the sediments, some of the sedimentary rocks (e.g., potassic rocks) cannot be paired with any igneous rocks analyzed so far. In contrast, many float rocks, which cannot be classified from their poorly defined texture, plot on chemistry diagrams close to float rocks defined as igneous from their textures, potentially constraining their nature.

  20. Classification scheme for sedimentary and igneous rocks in Gale crater, Mars

    DOE PAGES

    Mangold, Nicolas; Schmidt, Mariek E.; Fisk, Martin R.; ...

    2016-11-05

    Rocks analyzed by the Curiosity rover in Gale crater include a variety of clastic sedimentary rocks and igneous float rocks transported by fluvial and impact processes. Here, to facilitate the discussion of the range of lithologies, we present in this article a petrological classification framework adapting terrestrial classification schemes to Mars compositions (such as Fe abundances typically higher than for comparable lithologies on Earth), to specific Curiosity observations (such as common alkali-rich rocks), and to the capabilities of the rover instruments. Mineralogy was acquired only locally for a few drilled rocks, and so it does not suffice as a systematicmore » classification tool, in contrast to classical terrestrial rock classification. The core of this classification involves (1) the characterization of rock texture as sedimentary, igneous or undefined according to grain/crystal sizes and shapes using imaging from the ChemCam Remote Micro-Imager (RMI), Mars Hand Lens Imager (MAHLI) and Mastcam instruments, and (2) the assignment of geochemical modifiers based on the abundances of Fe, Si, alkali, and S determined by the Alpha Particle X-ray Spectrometer (APXS) and ChemCam instruments. The aims are to help understand Gale crater geology by highlighting the various categories of rocks analyzed by the rover. Several implications are proposed from the cross-comparisons of rocks of various texture and composition, for instance between in place outcrops and float rocks. All outcrops analyzed by the rover are sedimentary; no igneous outcrops have been observed. However, some igneous rocks are clasts in conglomerates, suggesting that part of them are derived from the crater rim. The compositions of in-place sedimentary rocks contrast significantly with the compositions of igneous float rocks. While some of the differences between sedimentary rocks and igneous floats may be related to physical sorting and diagenesis of the sediments, some of the sedimentary rocks (e.g., potassic rocks) cannot be paired with any igneous rocks analyzed so far. Finally, in contrast, many float rocks, which cannot be classified from their poorly defined texture, plot on chemistry diagrams close to float rocks defined as igneous from their textures, potentially constraining their nature.« less

  1. Classification scheme for sedimentary and igneous rocks in Gale crater, Mars

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mangold, Nicolas; Schmidt, Mariek E.; Fisk, Martin R.

    Rocks analyzed by the Curiosity rover in Gale crater include a variety of clastic sedimentary rocks and igneous float rocks transported by fluvial and impact processes. Here, to facilitate the discussion of the range of lithologies, we present in this article a petrological classification framework adapting terrestrial classification schemes to Mars compositions (such as Fe abundances typically higher than for comparable lithologies on Earth), to specific Curiosity observations (such as common alkali-rich rocks), and to the capabilities of the rover instruments. Mineralogy was acquired only locally for a few drilled rocks, and so it does not suffice as a systematicmore » classification tool, in contrast to classical terrestrial rock classification. The core of this classification involves (1) the characterization of rock texture as sedimentary, igneous or undefined according to grain/crystal sizes and shapes using imaging from the ChemCam Remote Micro-Imager (RMI), Mars Hand Lens Imager (MAHLI) and Mastcam instruments, and (2) the assignment of geochemical modifiers based on the abundances of Fe, Si, alkali, and S determined by the Alpha Particle X-ray Spectrometer (APXS) and ChemCam instruments. The aims are to help understand Gale crater geology by highlighting the various categories of rocks analyzed by the rover. Several implications are proposed from the cross-comparisons of rocks of various texture and composition, for instance between in place outcrops and float rocks. All outcrops analyzed by the rover are sedimentary; no igneous outcrops have been observed. However, some igneous rocks are clasts in conglomerates, suggesting that part of them are derived from the crater rim. The compositions of in-place sedimentary rocks contrast significantly with the compositions of igneous float rocks. While some of the differences between sedimentary rocks and igneous floats may be related to physical sorting and diagenesis of the sediments, some of the sedimentary rocks (e.g., potassic rocks) cannot be paired with any igneous rocks analyzed so far. Finally, in contrast, many float rocks, which cannot be classified from their poorly defined texture, plot on chemistry diagrams close to float rocks defined as igneous from their textures, potentially constraining their nature.« less

  2. Ubiquitin-mediated modulation of the cytoplasmic viral RNA sensor RIG-I.

    PubMed

    Oshiumi, Hiroyuki; Matsumoto, Misako; Seya, Tsukasa

    2012-01-01

    RIG-I-like receptors, including RIG-I, MDA5 and LGP2, recognize cytoplasmic viral RNA. The RIG-I protein consists of N-terminal CARDs, central RNA helicase and C-terminal domains. RIG-I activation is regulated by ubiquitination. Three ubiquitin ligases target the RIG-I protein. TRIM25 and Riplet ubiquitin ligases are positive regulators of RIG-I and deliver the K63-linked polyubiquitin moiety to RIG-I CARDs and the C-terminal domain. RNF125, another ubiquitin ligase, is a negative regulator of RIG-I and mediates K48-linked polyubiquitination of RIG-I, leading to the degradation of the RIG-I protein by proteasomes. The K63-linked polyubiquitin chains of RIG-I are removed by a deubiquitin enzyme, CYLD. Thus, CYLD is a negative regulator of RIG-I. Furthermore, TRIM25 itself is regulated by ubiquitination. HOIP and HOIL proteins are ubiquitin ligases and are also known as linear ubiquitin assembly complexes (LUBACs). The TRIM25 protein is ubiquitinated by LUBAC and then degraded by proteasomes. The splice variant of RIG-I encodes a protein that lacks the first CARD of RIG-I, and the variant RIG-I protein is not ubiquitinated by TRIM25. Therefore, ubiquitin is the key regulator of the cytoplasmic viral RNA sensor RIG-I.

  3. Simple, affordable, and sustainable borehole observatories for complex monitoring objectives

    NASA Astrophysics Data System (ADS)

    Kopf, A.; Freudenthal, T.; Ratmeyer, V.; Bergenthal, M.; Lange, M.; Fleischmann, T.; Hammerschmidt, S.; Seiter, C.; Wefer, G.

    2015-05-01

    Seafloor drill rigs are remotely operated systems that provide a cost-effective means to recover sedimentary records of the upper sub-seafloor deposits. Recent increases in their payload included downhole logging tools or autoclave coring systems. Here we report on another milestone in using seafloor rigs: the development and installation of shallow borehole observatories. Three different systems have been developed for the MARUM-MeBo (Meeresboden-Bohrgerat) seafloor drill, which is operated by MARUM, University of Bremen, Germany. A simple design, the MeBoPLUG, separates the inner borehole from the overlying ocean by using o-ring seals at the conical threads of the drill pipe. The systems are self-contained and include data loggers, batteries, thermistors and a differential pressure sensor. A second design, the so-called MeBoCORK (Circulation Obviation Retrofit Kit), is more sophisticated and also hosts an acoustic modem for data transfer and, if desired, fluid sampling capability using osmotic pumps. In these MeBoCORKs, two systems have to be distinguished: the CORK-A (A stands for autonomous) can be installed by the MeBo alone and monitors pressure and temperature inside and above the borehole (the latter for reference); the CORK-B (B stands for bottom) has a higher payload and can additionally be equipped with geochemical, biological or other physical components. Owing to its larger size, it is installed by a remotely operated underwater vehicle (ROV) and utilises a hot-stab connection in the upper portion of the drill string. Either design relies on a hot-stab connection from beneath in which coiled tubing with a conical drop weight is lowered to couple to the formation. These tubes are fluid-saturated and either serve to transmit pore pressure signals or collect porewater in the osmo-sampler. The third design, the MeBoPUPPI (Pop-Up Pore Pressure Instrument), is similar to the MeBoCORK-A and monitors pore pressure and temperature in a self-contained manner. Instead of transferring data on command using an acoustic modem, the MeBoPUPPI contains a pop-up telemetry with iridium link. After a predefined period, the data unit with satellite link is released, ascends to the sea surface, and remains there for up to 2 weeks while sending the long-term data sets to shore. In summer 2012, two MeBoPLUGs, one MeBoCORK-A and one MeBoCORK-B were installed with MeBo on RV Sonne, Germany, in the Nankai Trough area, Japan. We have successfully downloaded data from the CORKs, attesting that coupling to the formation worked, and pressure records were elevated relative to the seafloor reference. In the near future, we will further deploy the first two MeBoPUPPIs. Recovery of all monitoring systems by a ROV is planned for 2016.

  4. Lake Eĺ gygytgyn Drilling under way: State of the operation and first results

    NASA Astrophysics Data System (ADS)

    Melles, M.; Brigham-Grette, J.; Minyuk, P.; Koeberl, C.; Scientific Party, EĺGygytgyn

    2009-04-01

    Lake Eĺgygytgyn, located in central Chukotka, NE Siberia, was formed 3.6 million years ago by a meteorite impact and has never been glaciated or desiccated. This makes Lake Eĺgygytgyn a unique target of an interdisciplinary, multi-national drilling campaign, which currently is carried out as part of the International Continental Drilling Program (ICDP). Drilling operations started in Nov./Dec. 2008, when a 142 m long sediment core was retrieved from the permafrost deposits in the western lake catchment by the local drilling company Chaun Mine Geological Company (CGE). The core penetrated coarse-grained, ice-rich alluvial sediments with variable contents of fine-grained material. It will be investigated for the environmental history, including potential lake-level changes, and the permafrost characteristics, in order to learn more about the influences of catchment changes on the lake sedimentation. Besides, the hole was permanently instrumented for future ground temperature monitoring as part of the Global Terrestrial Network for Permafrost (www.gtnp.org/index_e.html). The major drilling effort will commence in Febr. 2009, when two sites in the central part of Lake Eĺgygytgyn shall be drilled down to 630 m below the lake floor. Drilling will be carried out by DOSECC, using a new GLAD 800 system that will be operated from an enclosed platform on the lake ice. Drilling objectives include replicate overlapping cores from the up to 420 m thick lake sediment fill. The cores promise to yield the longest, most continuous record of climate change in the terrestrial Arctic, extending back one million years prior to the intensification of the Northern Hemisphere Glaciation at the Pliocene/Pleistocene boundary, thus offering unique insight into the climatic and environmental history of the Arctic and its comparison with records from lower latitude marine and terrestrial sites to better understand hemispheric and global climate change. Coring shall be continued up to 300 m into the underlying impact breccia and brecciated bedrock in order to additionally investigate the impact process and the response of the volcanic bedrock to the impact event. The field season will continue into May, when surface melting on the lake will push to start evacuation of the drill rig. In summer 2009, the cores will be flown by chartered cargo plane to St. Petersburg. Later they will be trucked to the University of Cologne, Germany, for sub-sampling starting in September by the international team and their students. The archive core halves will be shipped to the University of Minnesota LacCore Facility in the US for post-moratorium studies. This talk will provide an introduction into the drilling objectives, summarize the first conclusions that can be drawn from the field data, and outline the next steps towards multidisciplinary investigation of the core material by the international science team.

  5. Downhole surge valve for earth boring apparatus

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lee, D.W.

    1990-05-29

    This patent describes a boring tool assembly having an underground percussion mole boring tool powered by a working fluid, the tool being driven through the earth by a rigid drill string pushed by a drilling frame, and a downhole valve assembly fixed between the downhole end of the drill string and the too, the improved downhole valve assembly. It comprises: a valve spool having an open first end, a closed second end and a peripheral sidewall, an axial bore extending partly through the valve spool from the open first end; a radial passage adjacent the closed second and of themore » valve spool, the radial passage extending radially from the valve spool axial bore through the valve spool peripheral sidewall; an axial groove in the peripheral sidewall of the valve spool; a valve body having a first end, a second end and a peripheral sidewall, an axial bore extending through the valve body, the valve spool extending through the valve body axial bore so that the second end of the valve body is adjacent the closed second end of the valve spool, the valve spool being axially moveable within the valve body axial bore; an axial slot; a free-floating key element; a valve housing; and seal means.« less

  6. Job stress, mental health, and accidents among offshore workers in the oil and gas extraction industries.

    PubMed

    Cooper, C L; Sutherland, V J

    1987-02-01

    Psychosocial and occupational stressors among 194 male employees on drilling rig and production platform installations in the United Kingdom and Dutch sectors of the North Sea were studied. Mental well-being and job satisfaction were also assessed, with attention to the incidence of accidents offshore. This occupational group were found to be much less satisfied with their jobs than their onshore counterparts. Although overall mental well-being compared favorably with that of the general population, levels of anxiety were significantly higher. Multivariate analysis showed "relationships at work and at home" to be a strong predictor of both job dissatisfaction and mental ill-health. Type A coronary-prone behavior was also found to be a significant predictor of reduced mental well-being and increased accident rates offshore.

  7. Conventional protein kinase C-α (PKC-α) and PKC-β negatively regulate RIG-I antiviral signal transduction.

    PubMed

    Maharaj, Natalya P; Wies, Effi; Stoll, Andrej; Gack, Michaela U

    2012-02-01

    Retinoic acid-inducible gene I (RIG-I) is a key sensor for viral RNA in the cytosol, and it initiates a signaling cascade that leads to the establishment of an interferon (IFN)-mediated antiviral state. Because of its integral role in immune signaling, RIG-I activity must be precisely controlled. Recent studies have shown that RIG-I CARD-dependent signaling function is regulated by the dynamic balance between phosphorylation and TRIM25-induced K₆₃-linked ubiquitination. While ubiquitination of RIG-I is critical for RIG-I's ability to induce an antiviral IFN response, phosphorylation of RIG-I at S₈ or T₁₇₀ suppresses RIG-I signal-transducing activity under normal conditions. Here, we not only further define the roles of S₈ and T₁₇₀ phosphorylation for controlling RIG-I activity but also identify conventional protein kinase C-α (PKC-α) and PKC-β as important negative regulators of the RIG-I signaling pathway. Mutational analysis indicated that while the phosphorylation of S₈ or T₁₇₀ potently inhibits RIG-I downstream signaling, the dephosphorylation of RIG-I at both residues is necessary for optimal TRIM25 binding and ubiquitination-mediated RIG-I activation. Furthermore, exogenous expression, gene silencing, and specific inhibitor treatment demonstrated that PKC-α/β are the primary kinases responsible for RIG-I S₈ and T₁₇₀ phosphorylation. Coimmunoprecipitation showed that PKC-α/β interact with RIG-I under normal conditions, leading to its phosphorylation, which suppresses TRIM25 binding, RIG-I CARD ubiquitination, and thereby RIG-I-mediated IFN induction. PKC-α/β double-knockdown cells exhibited markedly decreased S₈/T₁₇₀ phosphorylation levels of RIG-I and resistance to infection by vesicular stomatitis virus. Thus, these findings demonstrate that PKC-α/β-induced RIG-I phosphorylation is a critical regulatory mechanism for controlling RIG-I antiviral signal transduction under normal conditions.

  8. Conventional Protein Kinase C-α (PKC-α) and PKC-β Negatively Regulate RIG-I Antiviral Signal Transduction

    PubMed Central

    Maharaj, Natalya P.; Wies, Effi; Stoll, Andrej

    2012-01-01

    Retinoic acid-inducible gene I (RIG-I) is a key sensor for viral RNA in the cytosol, and it initiates a signaling cascade that leads to the establishment of an interferon (IFN)-mediated antiviral state. Because of its integral role in immune signaling, RIG-I activity must be precisely controlled. Recent studies have shown that RIG-I CARD-dependent signaling function is regulated by the dynamic balance between phosphorylation and TRIM25-induced K63-linked ubiquitination. While ubiquitination of RIG-I is critical for RIG-I's ability to induce an antiviral IFN response, phosphorylation of RIG-I at S8 or T170 suppresses RIG-I signal-transducing activity under normal conditions. Here, we not only further define the roles of S8 and T170 phosphorylation for controlling RIG-I activity but also identify conventional protein kinase C-α (PKC-α) and PKC-β as important negative regulators of the RIG-I signaling pathway. Mutational analysis indicated that while the phosphorylation of S8 or T170 potently inhibits RIG-I downstream signaling, the dephosphorylation of RIG-I at both residues is necessary for optimal TRIM25 binding and ubiquitination-mediated RIG-I activation. Furthermore, exogenous expression, gene silencing, and specific inhibitor treatment demonstrated that PKC-α/β are the primary kinases responsible for RIG-I S8 and T170 phosphorylation. Coimmunoprecipitation showed that PKC-α/β interact with RIG-I under normal conditions, leading to its phosphorylation, which suppresses TRIM25 binding, RIG-I CARD ubiquitination, and thereby RIG-I-mediated IFN induction. PKC-α/β double-knockdown cells exhibited markedly decreased S8/T170 phosphorylation levels of RIG-I and resistance to infection by vesicular stomatitis virus. Thus, these findings demonstrate that PKC-α/β-induced RIG-I phosphorylation is a critical regulatory mechanism for controlling RIG-I antiviral signal transduction under normal conditions. PMID:22114345

  9. Design and reliability analysis of DP-3 dynamic positioning control architecture

    NASA Astrophysics Data System (ADS)

    Wang, Fang; Wan, Lei; Jiang, Da-Peng; Xu, Yu-Ru

    2011-12-01

    As the exploration and exploitation of oil and gas proliferate throughout deepwater area, the requirements on the reliability of dynamic positioning system become increasingly stringent. The control objective ensuring safety operation at deep water will not be met by a single controller for dynamic positioning. In order to increase the availability and reliability of dynamic positioning control system, the triple redundancy hardware and software control architectures were designed and developed according to the safe specifications of DP-3 classification notation for dynamically positioned ships and rigs. The hardware redundant configuration takes the form of triple-redundant hot standby configuration including three identical operator stations and three real-time control computers which connect each other through dual networks. The function of motion control and redundancy management of control computers were implemented by software on the real-time operating system VxWorks. The software realization of task loose synchronization, majority voting and fault detection were presented in details. A hierarchical software architecture was planed during the development of software, consisting of application layer, real-time layer and physical layer. The behavior of the DP-3 dynamic positioning control system was modeled by a Markov model to analyze its reliability. The effects of variation in parameters on the reliability measures were investigated. The time domain dynamic simulation was carried out on a deepwater drilling rig to prove the feasibility of the proposed control architecture.

  10. Smaller Footprint Drilling System for Deep and Hard Rock Environments; Feasibility of Ultra-High-Speed Diamond Drilling

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    TerraTek, A Schlumberger Company

    2008-12-31

    The two phase program addresses long-term developments in deep well and hard rock drilling. TerraTek believes that significant improvements in drilling deep hard rock will be obtained by applying ultra-high rotational speeds (greater than 10,000 rpm). The work includes a feasibility of concept research effort aimed at development that will ultimately result in the ability to reliably drill 'faster and deeper' possibly with smaller, more mobile rigs. The principle focus is on demonstration testing of diamond bits rotating at speeds in excess of 10,000 rpm to achieve high rate of penetration (ROP) rock cutting with substantially lower inputs of energymore » and loads. The significance of the 'ultra-high rotary speed drilling system' is the ability to drill into rock at very low weights on bit and possibly lower energy levels. The drilling and coring industry today does not practice this technology. The highest rotary speed systems in oil field and mining drilling and coring today run less than 10,000 rpm - usually well below 5,000 rpm. This document provides the progress through two phases of the program entitled 'Smaller Footprint Drilling System for Deep and Hard Rock Environments: Feasibility of Ultra-High-Speed Diamond Drilling' for the period starting 30 June 2003 and concluding 31 March 2009. The accomplishments of Phases 1 and 2 are summarized as follows: (1) TerraTek reviewed applicable literature and documentation and convened a project kick-off meeting with Industry Advisors in attendance (see Black and Judzis); (2) TerraTek designed and planned Phase I bench scale experiments (See Black and Judzis). Improvements were made to the loading mechanism and the rotational speed monitoring instrumentation. New drill bit designs were developed to provided a more consistent product with consistent performance. A test matrix for the final core bit testing program was completed; (3) TerraTek concluded small-scale cutting performance tests; (4) Analysis of Phase 1 data indicated that there is decreased specific energy as the rotational speed increases; (5) Technology transfer, as part of Phase 1, was accomplished with technical presentations to the industry (see Judzis, Boucher, McCammon, and Black); (6) TerraTek prepared a design concept for the high speed drilling test stand, which was planned around the proposed high speed mud motor concept. Alternative drives for the test stand were explored; a high speed hydraulic motor concept was finally used; (7) The high speed system was modified to accommodate larger drill bits than originally planned; (8) Prototype mud turbine motors and the high speed test stand were used to drive the drill bits at high speed; (9) Three different rock types were used during the testing: Sierra White granite, Crab Orchard sandstone, and Colton sandstone. The drill bits used included diamond impregnated bits, a polycrystalline diamond compact (PDC) bit, a thermally stable PDC (TSP) bit, and a hybrid TSP and natural diamond bit; and (10) The drill bits were run at rotary speeds up to 5500 rpm and weight on bit (WOB) to 8000 lbf. During Phase 2, the ROP as measured in depth of cut per bit revolution generally increased with increased WOB. The performance was mixed with increased rotary speed, with the depth cut with the impregnated drill bit generally increasing and the TSP and hybrid TSP drill bits generally decreasing. The ROP in ft/hr generally increased with all bits with increased WOB and rotary speed. The mechanical specific energy generally improved (decreased) with increased WOB and was mixed with increased rotary speed.« less

  11. Viral Pseudo Enzymes Activate RIG-I via Deamidation to Evade Cytokine Production

    PubMed Central

    He, Shanping; Zhao, Jun; Song, Shanshan; He, Xiaojing; Minassian, Arlet; Zhou, Yu; Zhang, Junjie; Brulois, Kevin; Wang, Yuqi; Cabo, Jackson; Zandi, Ebrahim; Liang, Chengyu; Jung, Jae U; Zhang, Xuewu; Feng, Pinghui

    2015-01-01

    SUMMARY RIG-I is a pattern recognition receptor that senses viral RNA and is crucial for host innate immune defense. Here we describe a mechanism of RIG-I activation through amidotransferase-mediated deamidation. We show that viral homologues of phosphoribosylformyglycinamide synthase (PFAS), although lacking intrinsic enzyme activity, recruit cellular PFAS to deamidate and activate RIG-I. Accordingly, depletion and biochemical inhibition of PFAS impair RIG-I deamidation and concomitant activation. Purified PFAS and viral homologue thereof deamidate RIG-I in vitro. Ultimately, herpesvirus hijacks activated RIG-I to avoid antiviral cytokine production; loss of RIG-I or inhibition of RIG-I deamidation results in elevated cytokine production. Together, these findings demonstrate a surprising mechanism of RIG-I activation that is mediated by an enzyme. PMID:25752576

  12. Deployment and evaluation of a dual-sensor autofocusing method for on-machine measurement of patterns of small holes on freeform surfaces.

    PubMed

    Chen, Xiaomei; Longstaff, Andrew; Fletcher, Simon; Myers, Alan

    2014-04-01

    This paper presents and evaluates an active dual-sensor autofocusing system that combines an optical vision sensor and a tactile probe for autofocusing on arrays of small holes on freeform surfaces. The system has been tested on a two-axis test rig and then integrated onto a three-axis computer numerical control (CNC) milling machine, where the aim is to rapidly and controllably measure the hole position errors while the part is still on the machine. The principle of operation is for the tactile probe to locate the nominal positions of holes, and the optical vision sensor follows to focus and capture the images of the holes. The images are then processed to provide hole position measurement. In this paper, the autofocusing deviations are analyzed. First, the deviations caused by the geometric errors of the axes on which the dual-sensor unit is deployed are estimated to be 11 μm when deployed on a test rig and 7 μm on the CNC machine tool. Subsequently, the autofocusing deviations caused by the interaction of the tactile probe, surface, and small hole are mathematically analyzed and evaluated. The deviations are a result of the tactile probe radius, the curvatures at the positions where small holes are drilled on the freeform surface, and the effect of the position error of the hole on focusing. An example case study is provided for the measurement of a pattern of small holes on an elliptical cylinder on the two machines. The absolute sum of the autofocusing deviations is 118 μm on the test rig and 144 μm on the machine tool. This is much less than the 500 μm depth of field of the optical microscope. Therefore, the method is capable of capturing a group of clear images of the small holes on this workpiece for either implementation.

  13. Lithologic and ground-water-quality data collected using Hoverprobe drilling techniques at the West Branch Canal Creek wetland, Aberdeen Proving Ground, Maryland, April-May 2000

    USGS Publications Warehouse

    Phelan, Daniel J.; Senus, Michael P.; Olsen, Lisa D.

    2001-01-01

    This report presents lithologic and groundwater- quality data collected during April and May 2000 in the remote areas of the tidal wetland of West Branch Canal Creek, Aberdeen Proving Ground, Maryland. Contamination of the Canal Creek aquifer with volatile organic compounds has been documented in previous investigations of the area. This study was conducted to investigate areas that were previously inaccessible because of deep mud and shallow water, and to support ongoing investigations of the fate and transport of volatile organic compounds in the Canal Creek aquifer. A unique vibracore drill rig mounted on a hovercraft was used for drilling and groundwater sampling. Continuous cores of the wetland sediment and of the Canal Creek aquifer were collected at five sites. Attempts to sample ground water were made by use of a continuous profiler at 12 sites, without well installation, at a total of 81 depths within the aquifer. Of those 81 attempts, only 34 sampling depths produced enough water to collect samples. Ground-water samples from two sites had the highest concentrations of volatile organic compounds?with total volatile organic compound concentrations in the upper part of the aquifer ranging from about 15,000 to 50,000 micrograms per liter. Ground-water samples from five sites had much lower total volatile organic compound concentrations (95 to 2,100 micrograms per liter), whereas two sites were essentially not contaminated, with total volatile organic compound concentrations less than or equal to 5 micrograms per liter.

  14. Viral pseudo-enzymes activate RIG-I via deamidation to evade cytokine production.

    PubMed

    He, Shanping; Zhao, Jun; Song, Shanshan; He, Xiaojing; Minassian, Arlet; Zhou, Yu; Zhang, Junjie; Brulois, Kevin; Wang, Yuqi; Cabo, Jackson; Zandi, Ebrahim; Liang, Chengyu; Jung, Jae U; Zhang, Xuewu; Feng, Pinghui

    2015-04-02

    RIG-I is a pattern recognition receptor that senses viral RNA and is crucial for host innate immune defense. Here, we describe a mechanism of RIG-I activation through amidotransferase-mediated deamidation. We show that viral homologs of phosphoribosylformylglycinamidine synthetase (PFAS), although lacking intrinsic enzyme activity, recruit cellular PFAS to deamidate and activate RIG-I. Accordingly, depletion and biochemical inhibition of PFAS impair RIG-I deamidation and concomitant activation. Purified PFAS and viral homolog thereof deamidate RIG-I in vitro. Ultimately, herpesvirus hijacks activated RIG-I to avoid antiviral cytokine production; loss of RIG-I or inhibition of RIG-I deamidation results in elevated cytokine production. Together, these findings demonstrate a surprising mechanism of RIG-I activation that is mediated by an enzyme. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. Surface mapping and drilling of extinct seafloor massive sulphide deposits (eSMS) from the TAG Hydrothermal Field, 26oN: A tale of two `Jaspers'

    NASA Astrophysics Data System (ADS)

    Stobbs, I. J.; Lusty, P.; Petersen, S.; Murton, B. J.

    2017-12-01

    Two extinct seafloor massive sulphide (eSMS) deposits within the TAG hydrothermal field, 26oN, mid-Atlantic ridge, were mapped and drilled: Southern Mound and the newly discovered `Rona Mound'. Surface mapping was undertaken by combining high definition video footage and high resolution bathymetry to interpret surface geological and geomorphological features. Drill core was recovered using the BGS RD2 robotic drilling rig. Surface mapping of the mounds revealed a superficial cover of carbonate and iron-oxyhydroxides sediments, observed to directly overly oxide coated sulphide material within fault scarps, which dissect the flanks of both mounds. Drilling at the summits of the mounds revealed similar stratigraphy to the mapping, with the addition of a coherent and dense layer of red-coloured silica-rich `jasper', up to 3m thick, underlying the sediments and overlying unoxidised massive sulphides. The jasper mineralogy is dominated by silica, with minor iron oxides and rare disseminated sulphides. It displays a range of complex textures including filamentous and dendritic iron oxides often coated in silica. Drill core samples show the material to be porous, but relatively impermeable. Strong and positive Eu (REE) anomalies indicates a hydrothermal origin with little evidence of a seawater signature (lack of negative Ce anomaly). Silica precipitation is associated with low temperature hydrothermal activity, chert and jasper materials are locally present within the nearby hydrothermally active TAG mound and are more widespread at low-temperature diffuse hydrothermal sites such as within the MESO field. We interpret the `jasper' layers to be a common product, formed during the waning, low temperature, stage of the hydrothermal cycle which may form an impermeable and resistant `cap' that protects the underlying massive sulphide ore body from oxidation and dissolution. The formation of a `jasper cap' could act automatically to preserve eSMS deposits when hydrothermal circulation ceases and is essential to preserving the resource potential of eSMS deposits. This `jasper' capping layer is important from an economic perspective, and reinforces the need for shallow sub-seafloor mapping as part of any deep-sea mineral exploration. This research received funding from the EC FP7 project Blue Mining (604500).

  16. Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated antiviral signal transduction

    PubMed Central

    Gack, Michaela U.; Kirchhofer, Axel; Shin, Young C.; Inn, Kyung-Soo; Liang, Chengyu; Cui, Sheng; Myong, Sua; Ha, Taekjip; Hopfner, Karl-Peter; Jung, Jae U.

    2008-01-01

    The caspase recruitment domain (CARD) of intracellular adaptors and sensors plays a critical role in the assembly of signaling complexes involved in innate host defense against pathogens and in the regulation of inflammatory responses. The cytosolic receptor retinoic acid-inducible gene-I (RIG-I) recognizes viral RNA in a 5′-triphosphate-dependent manner and initiates an antiviral signaling cascade. Upon viral infection, the N-terminal CARDs of RIG-I undergo the K63-linked ubiquitination induced by tripartite motif protein 25 (TRIM25), critical for the interaction of RIG-I with its downstream signaling partner MAVS/VISA/IPS-1/Cardif. Here, we demonstrate the distinct roles of RIG-I first and second CARD in TRIM25-mediated RIG-I ubiquitination: TRIM25 binds the RIG-I first CARD and subsequently ubiquitinates its second CARD. The T55I mutation in RIG-I first CARD abolishes TRIM25 interaction, whereas the K172R mutation in the second CARD eliminates polyubiquitin attachment. The necessity of the intact tandem CARD for RIG-I function is further evidenced by a RIG-I splice variant (SV) whose expression is robustly up-regulated upon viral infection. The RIG-I SV carries a short deletion (amino acids 36–80) within the first CARD and thereby loses TRIM25 binding, CARD ubiquitination, and downstream signaling ability. Furthermore, because of its robust inhibition of virus-induced RIG-I multimerization and RIG-I-MAVS signaling complex formation, this SV effectively suppresses the RIG-I-mediated IFN-β production. This study not only elucidates the vital role of the intact tandem CARD for TRIM25-mediated RIG-I activation but also identifies the RIG-I SV as an off-switch regulator of its own signaling pathway. PMID:18948594

  17. Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated antiviral signal transduction.

    PubMed

    Gack, Michaela U; Kirchhofer, Axel; Shin, Young C; Inn, Kyung-Soo; Liang, Chengyu; Cui, Sheng; Myong, Sua; Ha, Taekjip; Hopfner, Karl-Peter; Jung, Jae U

    2008-10-28

    The caspase recruitment domain (CARD) of intracellular adaptors and sensors plays a critical role in the assembly of signaling complexes involved in innate host defense against pathogens and in the regulation of inflammatory responses. The cytosolic receptor retinoic acid-inducible gene-I (RIG-I) recognizes viral RNA in a 5'-triphosphate-dependent manner and initiates an antiviral signaling cascade. Upon viral infection, the N-terminal CARDs of RIG-I undergo the K(63)-linked ubiquitination induced by tripartite motif protein 25 (TRIM25), critical for the interaction of RIG-I with its downstream signaling partner MAVS/VISA/IPS-1/Cardif. Here, we demonstrate the distinct roles of RIG-I first and second CARD in TRIM25-mediated RIG-I ubiquitination: TRIM25 binds the RIG-I first CARD and subsequently ubiquitinates its second CARD. The T(55)I mutation in RIG-I first CARD abolishes TRIM25 interaction, whereas the K(172)R mutation in the second CARD eliminates polyubiquitin attachment. The necessity of the intact tandem CARD for RIG-I function is further evidenced by a RIG-I splice variant (SV) whose expression is robustly up-regulated upon viral infection. The RIG-I SV carries a short deletion (amino acids 36-80) within the first CARD and thereby loses TRIM25 binding, CARD ubiquitination, and downstream signaling ability. Furthermore, because of its robust inhibition of virus-induced RIG-I multimerization and RIG-I-MAVS signaling complex formation, this SV effectively suppresses the RIG-I-mediated IFN-beta production. This study not only elucidates the vital role of the intact tandem CARD for TRIM25-mediated RIG-I activation but also identifies the RIG-I SV as an off-switch regulator of its own signaling pathway.

  18. The development of the Garden Banks block 388 FPF mooring system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dove, P.G.S.; Librino, F.; Scovell, D.C.

    1995-12-01

    This paper discusses work conducted during the design, procurement and installation of Enserch Exploration, Inc.`s Garden Banks 388 FPF mooring system. The design began with the execution of a trade-off study evaluating and comparing previously installed floating production moorings in the Gulf of Mexico, coupled with evaluation of new concepts and emphasis on cost effective solutions. The design effort involved dynamic analysis and wind tunnel and model tank testing, all in accordance with the newly completed API document RP 2FP1. Inspection of various components from the Placid GC-29 FPS moorings (installed in 1987 and recovered in 1990) determined that sectionsmore » of chain, jacketed spiral strand wire rope, submersible buoys and connectors could be reused with suitable refurbishment. The excellent condition of the rig`s onboard winching system also resulted in the reuse of the windlasses, with specified upgrades. Because a sufficient amount of used wire was not available, a bare spiral strand wire rope construction was adopted, including zinc anodes in the new sections, rather than jacketed strand. The lack of cost effective installation vessels in the Gulf of Mexico at the time of the installation bid posed challenges to Enserch. However, an innovative preset mooring installation scheme involving Heeremac`s SSCV Balder on its own moorings was adopted. Since the vessel was already in the Gulf of Mexico on contract for other projects, a cost effective contract was negotiated. The results of this effort led to considerable cost savings for Enserch, compared to conventional FPF mooring systems previously installed in the Gulf of Mexico.« less

  19. Hawaii Scientific Drilling Project: Objectives, Successes, Surprises and Frustrations

    NASA Astrophysics Data System (ADS)

    Depaolo, D. J.; Stolper, E.; Thomas, D. M.

    2008-12-01

    The Hawaii Scientific Drilling Project (HSDP) is a long-running project undertaken with the objective of studying a mantle plume by drilling an extended sequence of lavas from a single Hawaiian volcano. The project originated with a proposal to NSF in late 1986 with the idea of drilling to the Moho under Hilo; the target depth was estimated at 12km, commensurate with the depth reached by the drilling program then being pursued by the USSR and that proposed in the U.S. for the southern Appalachians, and in line with the aspirations of the nascent DOSECC program. Subsequently, due to limitations in funding and reorganization of the drilling program into what later became the NSF Continental Dynamics Program, HSDP was re-scoped with the objective of drilling deeply enough (ca. 4.5km) to recover most of the eruptive history of a single volcano. The project first went to a pilot stage, which resulted in coring to a depth of 1.1km in late 1993. The pilot stage was relatively inexpensive (1M including science) and productive. Funding was then obtained from NSF and ICDP in 1995 (ca. 12M) with the objective of drilling to 4.5km. Drilling was originally planned for a five-year period, in two campaigns. The first campaign, in 1999, resulted in efficient coring to a depth of 3.1km over a period of 6 months; it used about 40 percent of the funds and was also highly productive. Deepening the hole below 3.1km turned out to be both difficult and expensive, although for interesting reasons. To facilitate deeper drilling the hole needed to be reamed to a larger diameter; but when this was done the well unexpectedly started to flow. We now know that there are several deep pressurized aquifers, with varying salt content, but these hydrological phenomena were totally unanticipated. A key finding, also unanticipated, is that cold seawater circulates through the volcanic pile in volumes sufficient to refrigerate the entire section below 700m depth to temperatures about 25 degrees below a normal geothermal gradient. In early 1999 when the first drilling campaign was organized, the price of oil was 10 USD (rigs and drilling crews were available and reasonably priced); in early 2003 when hole opening was being arranged, the price of oil was 30 USD, and for the coring campaigns in 2005 and 2007 it was 50 to 70 USD. For these reasons, and because trip times were longer and deeply buried pillow basalts more difficult to drill, the remainder of the project funds (and then some) were needed to deepen the hole from 3.1 to 3.5km. Nevertheless, the project obtained a nearly continuous, and virtually unweathered, core consisting of lava flows, hyaloclastite, minor intrusives and sediment from a 3260m section of the Mauna Kea volcano, covering an age range from 200 to over 600 ka. It also recovered a 250m and a 280m section of the Mauna Loa volcano. A wealth of geological, volcanological, petrological, geochemical, geomagnetic, geodynamic, hydrological, and geobiological data have come from the core and the well, and more are coming in. The unprecedented geochemical-petrological data sets are a major success, as is the fact that geochemists can work together, but the hoped-for detailed geochronology for the core has proven difficult to obtain.

  20. New insights into microbial responses to oil spills from the Deepwater Horizon incident

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mason, O.U.; Hazen, T.C.

    2011-06-15

    On April 20, 2010, a catastrophic eruption of methane caused the Deepwater Horizon exploratory drill rig drilling the Macondo Well in Mississippi Canyon Block 252 (MC252) to explode. The Deepwater Horizon oil spill was unprecendeted for several reasons: the volume of oil released; the spill duration; the well depth; the distance from the shore-line (77 km or about 50 miles); the type of oil (light crude); and the injection of dispersant directly at the wellhead. This study clearly demonstrated that there was a profound and significant response by certain members of the in situ microbial community in the deep-sea inmore » the Gulf of Mexico. In particular putative hydrocarbon degrading Bacteria appeared to bloom in response to the Deepwater Horizon oil spill, even though the temperature at these depths is never >5 C. As the plume aged the shifts in the microbial community on a temporal scale suggested that different, yet metabolically important members of the community were able to respond to a myriad of plume constituents, e.g. shifting from propane/ethane to alkanes and finally to methane. Thus, the biodegradation of hydrocarbons in the plume by Bacteria was a highly significant process in the natural attenuation of many compounds released during the Deepwater Horizon oil spill.« less

  1. Estimated Emissions from the Prime-Movers of Unconventional Natural Gas Well Development Using Recently Collected In-Use Data in the United States.

    PubMed

    Johnson, Derek; Heltzel, Robert; Nix, Andrew; Darzi, Mahdi; Oliver, Dakota

    2018-05-01

    Natural gas from shale plays dominates new production and growth. However, unconventional well development is an energy intensive process. The prime movers, which include over-the-road service trucks, horizontal drilling rigs, and hydraulic fracturing pumps, are predominately powered by diesel engines that impact air quality. Instead of relying on certification data or outdated emission factors, this model uses new in-use emissions and activity data combined with historical literature to develop a national emissions inventory. For the diesel only case, hydraulic fracturing engines produced the most NO x emissions, while drilling engines produced the most CO emissions, and truck engines produced the most THC emissions. By implementing dual-fuel and dedicated natural gas engines, total fuel energy consumed, CO 2 , CO, THC, and CH 4 emissions would increase, while NO x emissions, diesel fuel consumption, and fuel costs would decrease. Dedicated natural gas engines offered significant reductions in NO x emissions. Additional scenarios examined extreme cases of full fleet conversions. While deep market penetrations could reduce fuel costs, both technologies could significantly increase CH 4 emissions. While this model is based on a small sample size of engine configurations, data were collected during real in-use activity and is representative of real world activity.

  2. On the possible long-term fate of oil released in the deepwater horizon incident: estimated by ensembles of dye release simulations

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Maltrud, Mathew E.; Peacock, Synte L.; Visbeck, Martin

    2010-08-01

    We have conducted an ensemble of 20 simulations using a high-resolution global ocean model in which dye was continuously injected at the site of the Deepwater Horizon drilling rig for two months. We then extended these simulations for another four months to track the dispersal of the dye in the model. We have also performed five simulations in which dye was continuously injected at the site of the spill for four months and then run out to one year from the initial spill date. The experiments can elucidate the time and space scales of dispersal of polluted waters and alsomore » give a quantitative estimate of dilution rate, ignoring any sink terms such as chemical or biological degradation.« less

  3. Structural and biochemical studies of RIG-I antiviral signaling.

    PubMed

    Feng, Miao; Ding, Zhanyu; Xu, Liang; Kong, Liangliang; Wang, Wenjia; Jiao, Shi; Shi, Zhubing; Greene, Mark I; Cong, Yao; Zhou, Zhaocai

    2013-02-01

    Retinoic acid-inducible gene I (RIG-I) is an important pattern recognition receptor that detects viral RNA and triggers the production of type-I interferons through the downstream adaptor MAVS (also called IPS-1, CARDIF, or VISA). A series of structural studies have elaborated some of the mechanisms of dsRNA recognition and activation of RIG-I. Recent studies have proposed that K63-linked ubiquitination of, or unanchored K63-linked polyubiquitin binding to RIG-I positively regulates MAVS-mediated antiviral signaling. Conversely phosphorylation of RIG-I appears to play an inhibitory role in controlling RIG-I antiviral signal transduction. Here we performed a combined structural and biochemical study to further define the regulatory features of RIG-I signaling. ATP and dsRNA binding triggered dimerization of RIG-I with conformational rearrangements of the tandem CARD domains. Full length RIG-I appeared to form a complex with dsRNA in a 2:2 molar ratio. Compared with the previously reported crystal structures of RIG-I in inactive state, our electron microscopic structure of full length RIG-I in complex with blunt-ended dsRNA, for the first time, revealed an exposed active conformation of the CARD domains. Moreover, we found that purified recombinant RIG-I proteins could bind to the CARD domain of MAVS independently of dsRNA, while S8E and T170E phosphorylation-mimicking mutants of RIG-I were defective in binding E3 ligase TRIM25, unanchored K63-linked polyubiquitin, and MAVS regardless of dsRNA. These findings suggested that phosphorylation of RIG inhibited downstream signaling by impairing RIG-I binding with polyubiquitin and its interaction with MAVS.

  4. Negative role of RIG-I serine 8 phosphorylation in the regulation of interferon-beta production.

    PubMed

    Nistal-Villán, Estanislao; Gack, Michaela U; Martínez-Delgado, Gustavo; Maharaj, Natalya P; Inn, Kyung-Soo; Yang, Heyi; Wang, Rong; Aggarwal, Aneel K; Jung, Jae U; García-Sastre, Adolfo

    2010-06-25

    RIG-I (retinoic acid-inducible gene I) and TRIM25 (tripartite motif protein 25) have emerged as key regulatory factors to induce interferon (IFN)-mediated innate immune responses to limit viral replication. Upon recognition of viral RNA, TRIM25 E3 ligase binds the first caspase recruitment domain (CARD) of RIG-I and subsequently induces lysine 172 ubiquitination of the second CARD of RIG-I, which is essential for the interaction with downstream MAVS/IPS-1/CARDIF/VISA and, thereby, IFN-beta mRNA production. Although ubiquitination has emerged as a major factor involved in RIG-I activation, the potential contribution of other post-translational modifications, such as phosphorylation, to the regulation of RIG-I activity has not been addressed. Here, we report the identification of serine 8 phosphorylation at the first CARD of RIG-I as a negative regulatory mechanism of RIG-I-mediated IFN-beta production. Immunoblot analysis with a phosphospecific antibody showed that RIG-I serine 8 phosphorylation steady-state levels were decreased upon stimulation of cells with IFN-beta or virus infection. Substitution of serine 8 in the CARD RIG-I functional domain with phosphomimetic aspartate or glutamate results in decreased TRIM25 binding, RIG-I ubiquitination, MAVS binding, and downstream signaling. Finally, sequence comparison reveals that only primate species carry serine 8, whereas other animal species carry an asparagine, indicating that serine 8 phosphorylation may represent a primate-specific regulation of RIG-I activation. Collectively, these data suggest that the phosphorylation of RIG-I serine 8 operates as a negative switch of RIG-I activation by suppressing TRIM25 interaction, further underscoring the importance of RIG-I and TRIM25 connection in type I IFN signal transduction.

  5. A Hierarchical Mechanism of RIG-I Ubiquitination Provides Sensitivity, Robustness and Synergy in Antiviral Immune Responses.

    PubMed

    Sun, Xiaoqiang; Xian, Huifang; Tian, Shuo; Sun, Tingzhe; Qin, Yunfei; Zhang, Shoutao; Cui, Jun

    2016-07-08

    RIG-I is an essential receptor in the initiation of the type I interferon (IFN) signaling pathway upon viral infection. Although K63-linked ubiquitination plays an important role in RIG-I activation, the optimal modulation of conjugated and unanchored ubiquitination of RIG-I as well as its functional implications remains unclear. In this study, we determined that, in contrast to the RIG-I CARD domain, full-length RIG-I must undergo K63-linked ubiquitination at multiple sites to reach full activity. A systems biology approach was designed based on experiments using full-length RIG-I. Model selection for 7 candidate mechanisms of RIG-I ubiquitination inferred a hierarchical architecture of the RIG-I ubiquitination mode, which was then experimentally validated. Compared with other mechanisms, the selected hierarchical mechanism exhibited superior sensitivity and robustness in RIG-I-induced type I IFN activation. Furthermore, our model analysis and experimental data revealed that TRIM4 and TRIM25 exhibited dose-dependent synergism. These results demonstrated that the hierarchical mechanism of multi-site/type ubiquitination of RIG-I provides an efficient, robust and optimal synergistic regulatory module in antiviral immune responses.

  6. A Hierarchical Mechanism of RIG-I Ubiquitination Provides Sensitivity, Robustness and Synergy in Antiviral Immune Responses

    PubMed Central

    Sun, Xiaoqiang; Xian, Huifang; Tian, Shuo; Sun, Tingzhe; Qin, Yunfei; Zhang, Shoutao; Cui, Jun

    2016-01-01

    RIG-I is an essential receptor in the initiation of the type I interferon (IFN) signaling pathway upon viral infection. Although K63-linked ubiquitination plays an important role in RIG-I activation, the optimal modulation of conjugated and unanchored ubiquitination of RIG-I as well as its functional implications remains unclear. In this study, we determined that, in contrast to the RIG-I CARD domain, full-length RIG-I must undergo K63-linked ubiquitination at multiple sites to reach full activity. A systems biology approach was designed based on experiments using full-length RIG-I. Model selection for 7 candidate mechanisms of RIG-I ubiquitination inferred a hierarchical architecture of the RIG-I ubiquitination mode, which was then experimentally validated. Compared with other mechanisms, the selected hierarchical mechanism exhibited superior sensitivity and robustness in RIG-I-induced type I IFN activation. Furthermore, our model analysis and experimental data revealed that TRIM4 and TRIM25 exhibited dose-dependent synergism. These results demonstrated that the hierarchical mechanism of multi-site/type ubiquitination of RIG-I provides an efficient, robust and optimal synergistic regulatory module in antiviral immune responses. PMID:27387525

  7. A Hierarchical Mechanism of RIG-I Ubiquitination Provides Sensitivity, Robustness and Synergy in Antiviral Immune Responses

    NASA Astrophysics Data System (ADS)

    Sun, Xiaoqiang; Xian, Huifang; Tian, Shuo; Sun, Tingzhe; Qin, Yunfei; Zhang, Shoutao; Cui, Jun

    2016-07-01

    RIG-I is an essential receptor in the initiation of the type I interferon (IFN) signaling pathway upon viral infection. Although K63-linked ubiquitination plays an important role in RIG-I activation, the optimal modulation of conjugated and unanchored ubiquitination of RIG-I as well as its functional implications remains unclear. In this study, we determined that, in contrast to the RIG-I CARD domain, full-length RIG-I must undergo K63-linked ubiquitination at multiple sites to reach full activity. A systems biology approach was designed based on experiments using full-length RIG-I. Model selection for 7 candidate mechanisms of RIG-I ubiquitination inferred a hierarchical architecture of the RIG-I ubiquitination mode, which was then experimentally validated. Compared with other mechanisms, the selected hierarchical mechanism exhibited superior sensitivity and robustness in RIG-I-induced type I IFN activation. Furthermore, our model analysis and experimental data revealed that TRIM4 and TRIM25 exhibited dose-dependent synergism. These results demonstrated that the hierarchical mechanism of multi-site/type ubiquitination of RIG-I provides an efficient, robust and optimal synergistic regulatory module in antiviral immune responses.

  8. Laguna Potrok Aike, Argentina: the first non-tropical environmental record in South America extending far beyond the Late-Glacial - a progress report

    NASA Astrophysics Data System (ADS)

    Zolitschka, B.; Anselmetti, F.; Ariztegui, D.; Corbella, H.; Francus, P.; Gebhardt, C.; Lücke, A.; Ohlendorf, C.; Schäbitz, F.; Pasado Science Team

    2009-04-01

    Within the framework of the ICDP-funded "Potrok Aike maar lake sediment archive drilling project" (PASADO) an international team of scientists carried out interdisciplinary research at the unique mid-Pleistocene (770 ka) maar lake of Laguna Potrok Aike in southern Patagonia (Province of Santa Cruz, Argentina). This lake is very sensitive to variations in southern hemispheric wind and pressure systems and thus holds a unique and continuous lacustrine record of climatic and ecological variability of global significance. Moreover, Southern Patagonia with its many active volcanoes is an ideal location to better understand the regional history of volcanism. These are two challenging geo-scientific themes that need to be tackled, especially as both of them have an increasing socio-economic relevance. Three months of drilling activities that finished last November 2008 were carried out by DOSECC from the drilling platform R/V "Kerry Kelts". More than 500 m of lacustrine sediments were recovered. This sedimentary archive will provide (1) new insights into the processes of regional back arc volcanism within the Pali Aike Volcanic Field itself as well as the more distant explosive volcanism of the Andean mountain chains; and, (2) high-resolution (decadal) quantitative climate and environmental reconstructions supported by multiple dating and stratigraphic correlations. Marine - ice core - terrestrial linkages will be emphasized as well as the incorporation of results from global climate modelling simulations for the last ca. 100 ka. The two drilled sites in the central deep basin of Laguna Potrok Aike have been selected based on four seismic surveys carried out between 2003 and 2005. Sediments were recovered at both drilled sites down to a subbottom depth of slightly more than 100 m using the GLAD800 drill rig with the hydraulic piston corer tool (HPC) at water depths varying between 95 and 100 m. The total core recovery is 94%. On-site core logging with the multi sensor core logger (MSCL) documents an excellent correlation between the four recovered holes drilled at Site 1 as well as with the three holes obtained from Site 2 which is located ca. 700 m south of Site 1. Additionally, a variety of sedimentological, physical and geochemical analyses were carried out on the core catcher samples in the field laboratory. Preliminary interpretation of all data that is available before core opening indicates that the record may go back in time as far as to the ending of oxygen isotope stage 5. Acknowledgements This research is supported by the International Continental Scientific Drilling Program (ICDP) in the framework of the "Potrok Aike Maar Lake Sediment Archive Drilling Project" (PASADO). Funding was provided by the ICDP, the German Science Foundation (DFG), the Swiss National Science Foundation (SNF), the Natural Sciences and Engineering Research Council of Canada (NSERC), the Swedish Vetenskapsradet (VR) and the University of Bremen.

  9. Riplet/RNF135, a RING finger protein, ubiquitinates RIG-I to promote interferon-beta induction during the early phase of viral infection.

    PubMed

    Oshiumi, Hiroyuki; Matsumoto, Misako; Hatakeyama, Shigetsugu; Seya, Tsukasa

    2009-01-09

    RIG-I (retinoic acid-inducible gene-I), a cytoplasmic RNA helicase, interacts with IPS-1/MAVS/Cardif/VISA, a protein on the outer membrane of mitochondria, to signal the presence of virus-derived RNA and induce type I interferon production. Activation of RIG-I requires the ubiquitin ligase, TRIM25, which mediates lysine 63-linked polyubiquitination of the RIG-I N-terminal CARD-like region. However, how this modification proceeds for activation of IPS-1 by RIG-I remains unclear. Here we identify an alternative factor, Riplet/RNF135, that promotes RIG-I activation independent of TRIM25. The Riplet/RNF135 protein consists of an N-terminal RING finger domain, C-terminal SPRY and PRY motifs, and shows sequence similarity to TRIM25. Immunoprecipitation analyses demonstrated that the C-terminal helicase and repressor domains of RIG-I interact with the Riplet/RNF135 C-terminal region, whereas the CARD-like region of RIG-I is dispensable for this interaction. Riplet/RNF135 promotes lysine 63-linked polyubiquitination of the C-terminal region of RIG-I, modification of which differs from the N-terminal ubiquitination by TRIM25. Overexpression and knockdown analyses revealed that Riplet/RNF135 promotes RIG-I-mediated interferon-beta promoter activation and inhibits propagation of the negative-strand RNA virus, vesicular stomatitis virus. Our data suggest that Riplet/RNF135 is a novel factor of the RIG-I pathway that is involved in the evoking of human innate immunity against RNA virus infection, and activates RIG-I through ubiquitination of its C-terminal region. We infer that a variety of RIG-I-ubiquitinating molecular complexes sustain RIG-I activation to modulate RNA virus replication in the cytoplasm.

  10. DOE Office of Scientific and Technical Information (OSTI.GOV)

    E Nistal-Villan; M Gack; G Martinez-Delgado

    RIG-I (retinoic acid-inducible gene I) and TRIM25 (tripartite motif protein 25) have emerged as key regulatory factors to induce interferon (IFN)-mediated innate immune responses to limit viral replication. Upon recognition of viral RNA, TRIM25 E3 ligase binds the first caspase recruitment domain (CARD) of RIG-I and subsequently induces lysine 172 ubiquitination of the second CARD of RIG-I, which is essential for the interaction with downstream MAVS/IPS-1/CARDIF/VISA and, thereby, IFN-beta mRNA production. Although ubiquitination has emerged as a major factor involved in RIG-I activation, the potential contribution of other post-translational modifications, such as phosphorylation, to the regulation of RIG-I activity hasmore » not been addressed. Here, we report the identification of serine 8 phosphorylation at the first CARD of RIG-I as a negative regulatory mechanism of RIG-I-mediated IFN-beta production. Immunoblot analysis with a phosphospecific antibody showed that RIG-I serine 8 phosphorylation steady-state levels were decreased upon stimulation of cells with IFN-beta or virus infection. Substitution of serine 8 in the CARD RIG-I functional domain with phosphomimetic aspartate or glutamate results in decreased TRIM25 binding, RIG-I ubiquitination, MAVS binding, and downstream signaling. Finally, sequence comparison reveals that only primate species carry serine 8, whereas other animal species carry an asparagine, indicating that serine 8 phosphorylation may represent a primate-specific regulation of RIG-I activation. Collectively, these data suggest that the phosphorylation of RIG-I serine 8 operates as a negative switch of RIG-I activation by suppressing TRIM25 interaction, further underscoring the importance of RIG-I and TRIM25 connection in type I IFN signal transduction.« less

  11. The mitochondrial targeting chaperone 14-3-3ε regulates a RIG-I translocon that mediates membrane-association and innate antiviral immunity

    PubMed Central

    Liu, Helene Minyi; Loo, Yueh-Ming; Horner, Stacy M.; Zornetzer, Gregory A.; Katze, Michael G.; Gale, Michael

    2012-01-01

    Summary RIG-I is a cytosolic pathogen recognition receptor that initiates immune responses against RNA viruses. Upon viral RNA recognition, anti-viral signalling requires RIG-I redistribution from the cytosol to membranes where it binds the adaptor protein, MAVS. Here we identify the mitochondrial targeting chaperone protein, 14-3-3ε, as a RIG-I-binding partner and essential component of a translocation complex or “translocon” containing RIG-I, 14-3-3ε, and the TRIM25 ubiquitin ligase. The RIG-I translocon directs RIG-I redistribution from the cytosol to membranes where it mediates MAVS-dependent innate immune signalling during acute RNA virus infection. 14-3-3ε is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. Our results define 14-3-3ε as a key component of a RIG-I translocon required for innate antiviral immunity. PMID:22607805

  12. The mitochondrial targeting chaperone 14-3-3ε regulates a RIG-I translocon that mediates membrane association and innate antiviral immunity.

    PubMed

    Liu, Helene Minyi; Loo, Yueh-Ming; Horner, Stacy M; Zornetzer, Gregory A; Katze, Michael G; Gale, Michael

    2012-05-17

    RIG-I is a cytosolic pathogen recognition receptor that initiates immune responses against RNA viruses. Upon viral RNA recognition, antiviral signaling requires RIG-I redistribution from the cytosol to membranes where it binds the adaptor protein, MAVS. Here we identify the mitochondrial targeting chaperone protein, 14-3-3ε, as a RIG-I-binding partner and essential component of a translocation complex or "translocon" containing RIG-I, 14-3-3ε, and the TRIM25 ubiquitin ligase. The RIG-I translocon directs RIG-I redistribution from the cytosol to membranes where it mediates MAVS-dependent innate immune signaling during acute RNA virus infection. 14-3-3ε is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. Our results define 14-3-3ε as a key component of a RIG-I translocon required for innate antiviral immunity. Copyright © 2012 Elsevier Inc. All rights reserved.

  13. Influence of backup bearings and support structure dynamics on the behavior of rotors with active supports

    NASA Technical Reports Server (NTRS)

    Flowers, George T.

    1994-01-01

    Progress over the past year includes the following: A simplified rotor model with a flexible shaft and backup bearings has been developed. A simple rotor model which includes a flexible disk and bearings with clearance has been developed and the dynamics of the model investigated. A rotor model based upon the T-501 engine has been developed which includes backup bearing effects. Parallel simulation runs are being conducted using an ANSYS based finite element model of the T-501. The magnetic bearing test rig is currently floating and dynamics/control tests are being conducted. A paper has been written that documents the work using the T-501 engine model. Work has continued with the simplified model. The finite element model is currently being modified to include the effects of foundation dynamics. A literature search for material on foil bearings has been conducted. A finite element model is being developed for a magnetic bearing in series with a foil backup bearing.

  14. A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses.

    PubMed

    Oshiumi, Hiroyuki; Miyashita, Moeko; Matsumoto, Misako; Seya, Tsukasa

    2013-01-01

    The innate immune system is essential for controlling viral infections, but several viruses have evolved strategies to escape innate immunity. RIG-I is a cytoplasmic viral RNA sensor that triggers the signal to induce type I interferon production in response to viral infection. RIG-I activation is regulated by the K63-linked polyubiquitin chain mediated by Riplet and TRIM25 ubiquitin ligases. TRIM25 is required for RIG-I oligomerization and interaction with the IPS-1 adaptor molecule. A knockout study revealed that Riplet was essential for RIG-I activation. However the molecular mechanism underlying RIG-I activation by Riplet remains unclear, and the functional differences between Riplet and TRIM25 are also unknown. A genetic study and a pull-down assay indicated that Riplet was dispensable for RIG-I RNA binding activity but required for TRIM25 to activate RIG-I. Mutational analysis demonstrated that Lys-788 within the RIG-I repressor domain was critical for Riplet-mediated K63-linked polyubiquitination and that Riplet was required for the release of RIG-I autorepression of its N-terminal CARDs, which leads to the association of RIG-I with TRIM25 ubiquitin ligase and TBK1 protein kinase. Our data indicate that Riplet is a prerequisite for TRIM25 to activate RIG-I signaling. We investigated the biological importance of this mechanism in human cells and found that hepatitis C virus (HCV) abrogated this mechanism. Interestingly, HCV NS3-4A proteases targeted the Riplet protein and abrogated endogenous RIG-I polyubiquitination and association with TRIM25 and TBK1, emphasizing the biological importance of this mechanism in human antiviral innate immunity. In conclusion, our results establish that Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein.

  15. A Distinct Role of Riplet-Mediated K63-Linked Polyubiquitination of the RIG-I Repressor Domain in Human Antiviral Innate Immune Responses

    PubMed Central

    Oshiumi, Hiroyuki; Miyashita, Moeko; Matsumoto, Misako; Seya, Tsukasa

    2013-01-01

    The innate immune system is essential for controlling viral infections, but several viruses have evolved strategies to escape innate immunity. RIG-I is a cytoplasmic viral RNA sensor that triggers the signal to induce type I interferon production in response to viral infection. RIG-I activation is regulated by the K63-linked polyubiquitin chain mediated by Riplet and TRIM25 ubiquitin ligases. TRIM25 is required for RIG-I oligomerization and interaction with the IPS-1 adaptor molecule. A knockout study revealed that Riplet was essential for RIG-I activation. However the molecular mechanism underlying RIG-I activation by Riplet remains unclear, and the functional differences between Riplet and TRIM25 are also unknown. A genetic study and a pull-down assay indicated that Riplet was dispensable for RIG-I RNA binding activity but required for TRIM25 to activate RIG-I. Mutational analysis demonstrated that Lys-788 within the RIG-I repressor domain was critical for Riplet-mediated K63-linked polyubiquitination and that Riplet was required for the release of RIG-I autorepression of its N-terminal CARDs, which leads to the association of RIG-I with TRIM25 ubiquitin ligase and TBK1 protein kinase. Our data indicate that Riplet is a prerequisite for TRIM25 to activate RIG-I signaling. We investigated the biological importance of this mechanism in human cells and found that hepatitis C virus (HCV) abrogated this mechanism. Interestingly, HCV NS3-4A proteases targeted the Riplet protein and abrogated endogenous RIG-I polyubiquitination and association with TRIM25 and TBK1, emphasizing the biological importance of this mechanism in human antiviral innate immunity. In conclusion, our results establish that Riplet-mediated K63-linked polyubiquitination released RIG-I RD autorepression, which allowed the access of positive factors to the RIG-I protein. PMID:23950712

  16. Radioimmunoguided surgery for colorectal cancer.

    PubMed

    Bertsch, D J; Burak, W E; Young, D C; Arnold, M W; Martin, E W

    1996-05-01

    Operations for patients with colorectal cancer are based on traditions established by historical experience. Radioimmunoguided surgery (RIGS) provides new information that challenges these traditions. Thirty-two patients with primary colorectal cancer underwent RIGS after being injected with anti-TAG-72 murine monoclonal antibody CC49 labeled with iodine-125. Sixteen of the patients had all gross tumor and RIGS-positive tissue removed (RIGS-negative group), and 16 had only traditional extirpation of the tumor because RIGS-positive tissue was too diffuse (RIGS-positive group). In the 16 patients having all RIGS-positive tissue removed, five had traditional regional en bloc resections and 11 had additional extraregional tissues resected. Identification of extraregional disease added two liver resections and 25 lymphadenectomies: 10 of the gastrohepatic ligament, five celia axis, six retroperitoneal, and four iliac. With a median follow-up of 37 months, survival in the RIGS-negative group is 100%. In 14 of 16 patients (87.5%) there is no evidence of disease. In the RIGS-positive group, follow-up shows 14 of 16 patients are dead and two are alive with disease (p < 0.0001). These results suggest that RIGS identifies patterns of disease dissemination different from those identified by traditional staging techniques. Removal of additional RIGS-positive tissues in nontraditional areas may improve survival.

  17. An innovative fast-track development for Cote d`Ivoire

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kreider, G.J.; Barnes, J.E.; Smitherman, J.E.

    1996-12-31

    In May, 1994, approval was received for the development of the Panthere Gas Field and Lion Oil Field by UMC Petroleum Corporation as operator. At that time, UMC had drilled one well from a jackup at Panthere in 270 feet of water and had set a guyed caisson. Following the Panthere gas discovery, the Lion oil discovery well was drilled in 235 feet of water and another guyed caisson was set. Paragon Engineering Services had been hired in late 1993 to develop project scenarios and cost estimates and as the project evolved, detailed engineering, procurement and construction management services. Productionmore » equipment for 30,000 BOPD, 16,000 BWPD and 75 MMSCFD was to be installed on a jackup drilling rig converted to a Mobile Offshore Production Unit (MOPU) located at the Lion A caisson. Production from Panthere would flow through an 8-inch line to the MOPU. The production equipment on the MOPU would (1) separate oil, water and gas, (2) treat the oil for sale and the water for discharge, (3) pump the oil into an 8-inch pipeline to the refinery in Abidjan, (4) compress the oil well and gas well gas as needed, and (5) dehydrate it for sale. A CALM buoy with shuttle tanker was included in the original plan for early production and as a backup to the oil pipeline. It was also unknown at project initiation whether a gas sales contract would be successfully negotiated, and thus the project was to proceed on the basis of flaring produced gas with capability to add equipment at a later date for gas sales. This paper describes how the project was organized and how this organization allowed the project to accommodate changes and construction problems as they developed while completing the project on time.« less

  18. The CARBONATE project: Mid-latitude Carbonate Systems - Complete Sequences from Cold-Water Coral Carbonate Mounds in the Northeast Atlantic

    NASA Astrophysics Data System (ADS)

    Wheeler, A.; Freiwald, A.; Hebbeln, D.; Swennen, R.; van Weering, T.; de Haas, H.; Dorschel, B.

    2007-12-01

    Up to now the carbonate stored in carbonate mounds has not been considered in any global carbonate budget or linked to any global carbon budget involving greenhouse gases. A major challenge exists to quantify the amount and flux of carbon stored by these newly discovered areas of enhanced carbonate accumulation in intermediate water depth. Furthermore, investigations so far reveal that all mounds possess different growth histories depending on the environmental setting and the involved faunal associations. Unfortunately, existing cores only penetrated the upper few meters of the mounds thus limiting mound research to the very late stage of mound development. Access to the longer sequences preserved in giant carbonate mounds was overcome in May 2005 when the IODP Expedition 307 (Porcupine Mound Drilling) recovered complete sedimentary records from one 155 m high "Challenger Mound" in the Porcupine Seabight west off Ireland. Furthermore, EU-FP projects have revealed late stage history of giant mounds in different settings showing that different mounds respond in different ways to environmental forcing factors with no one mound being typical of all. CARBONATE will drill complete sequences through a number of mounds in differing environmental settings using the portable drill rig MeBo (University of Bremen). By understanding how biogeochemical processes control the development of these carbonate mounds and their response to climate change, we will make an important step in quantifying their role as mid-latitude carbonate sinks. In the end, a better understanding of the processes involved in mound formation and development may also result in new views on fossil analogues many of which are less accessible hydrocarbon reservoirs.

  19. Estimating Western U.S. Oil & Gas Emissions with OMI NO2 Data

    NASA Astrophysics Data System (ADS)

    Clifton, O. E.; Holloway, T.; Oberman, J.

    2012-12-01

    In the last ten years, there has been a steep increase in the number natural gas and oil extraction facilities in the United States due to hydraulic fracturing ("fracking"). Each facility requires a large range of equipment, such as drilling rigs, compressor engines, heaters, and pneumatic devices. These activities can lead to elevated nitrogen dioxide (NO2) emissions in rural areas, often in regions without routine NO2 surface monitoring. Furthermore, permitting rules vary from state to state, and many new extraction facilities are unpermitted and exact emissions unknown. On April 18, 2012, the EPA announced air pollution standards for volatile organic compounds (VOCs) emissions from the oil and gas industry. Until 2015, when these standards must be in effect, NOx (NO2 + NO) will continue to react with VOCs to form unhealthy levels of tropospheric ozone in regions with heavy use of hydraulic fracturing. In order to identify areas of elevated NO2 emissions and constrain associated on-road and off-road sources in areas with prominent shale basins and known drilling, we employ remote sensing estimates of column NO2 from the Ozone Monitoring Instrument (OMI) aboard NASA's Aura satellite. OMI NO2 is sensitive to the planetary boundary layer and to surface air pollution and thus has high temporal and spatial variation. These Level-2 satellite data are processed with the Wisconsin Horizontal Interpolation Program for Satellites (WHIPS), developed at the University of Wisconsin-Madison. We interpolate the data to allow further ease in mapping change in NO2 associated with drilling, and the quantification of pollution trends attributable to hydraulic-fracturing in the Western U.S. from 2004 to the present.

  20. The ICDP Information Network and the Chinese Continental Scientific Drilling CCSD

    NASA Astrophysics Data System (ADS)

    Conze, R.; Su, D.

    2002-12-01

    ICDP is an international program investigating the 'System Earth' in multidisciplinary co-operation. Funded drilling projects are characterized by detailed fieldwork at world-class geological sites on the continents and by the global scope of research objectives. During project work, partnering researchers from all over the world work together at remote drill sites and in laboratories at their institutions. Researchers apply a range of highly diverse scientific methodologies, thereby acquiring huge data sets. Multinational co-operation and increasing amounts of scientific data require completely new concepts and practices for scientific work, and place heavy demands on information and communications management. This is achieved by means of the ICDP Information Network. Scientists working on ICDP related data need a central long-term data archive with powerful tools for navigation, data modeling and analysis. The Chinese Continental Scientific Drilling CCSD is a national key scientific and engineering project of the PR China supported by ICDP. The current drill site of CCSD is located in Donghai, Jiangsu Province, the eastern part of the Dabie-Sulu UHP metamorphic belt, which possesses global geological significance. From the spud on June 25, 2001 to April 6, 2002, the 2000m pilot hole was finished with a total core recovery of 88.7% and an average inclination angle of 3-4 degrees. The pilot hole has been transformed to the main hole by hole opening. Deepening and coring of the CCSD-1 main hole is currently in progress. Most of the basic scientific documentation and measurements are done in a large field laboratory directly beside the drill rig, which was set up using the standard of the former German Continental Scientific Drilling (KTB). It includes a powerful infrastructure for computing and electronic communication as well as a comprehensive twofold data and information management: 1. The CCSD-DMIS is a special Data Management Information System for the chinese project management, which is used for internal controlling and decision making. 2. The CCSD-DIS is the specifically designed on-site Drilling Information System, which is used for documentation and archiving of all kinds of scientific and technical information. Both are used in a local Intranet within the field lab, but they also provide certain information via secured Internet services. The CCSD-DIS feeds day-by-day the current reports and new recordings to the CCSD Web portal within the ICDP Information Network (http://www.icdp-online.org/html/sites/donghai/news/news.html). This portal provides chinese and english news and information for the public as well as scientific and technical stuff which is only available for the international CCSD Science Team. Using the example of the CCSD project, a poster and an on-line presentation will show the main components and value-added services of the ICDP Information Network like: ú the common portal for and dissemination of project information by the ICDP Clearinghouse, ú capture of scientific drilling data using individual On-Site Drilling Information Systems (DIS), ú virtual global field laboratories based on eXtended DIS, ú integrated evaluation and analysis of data supported by the ICDP Data Webhouse.

  1. Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.

    PubMed

    Liu, Wei; Li, Jing; Zheng, Weinan; Shang, Yingli; Zhao, Zhendong; Wang, Shanshan; Bi, Yuhai; Zhang, Shuang; Xu, Chongfeng; Duan, Ziyuan; Zhang, Lianfeng; Wang, Yue L; Jiang, Zhengfan; Liu, Wenjun; Sun, Lei

    2017-06-08

    RIG-I is a key cytosolic pattern recognition receptor that interacts with MAVS to induce type I interferons (IFNs) against RNA virus infection. In this study, we found that cyclophilin A (CypA), a peptidyl-prolyl cis/trans isomerase, functioned as a critical positive regulator of RIG-I-mediated antiviral immune responses. Deficiency of CypA impaired RIG-I-mediated type I IFN production and promoted viral replication in human cells and mice. Upon Sendai virus infection, CypA increased the interaction between RIG-I and its E3 ubiquitin ligase TRIM25, leading to enhanced TRIM25-mediated K63-linked ubiquitination of RIG-I that facilitated recruitment of RIG-I to MAVS. In addition, CypA and TRIM25 competitively interacted with MAVS, thereby inhibiting TRIM25-induced K48-linked ubiquitination of MAVS. Taken together, our findings reveal an essential role of CypA in boosting RIG-I-mediated antiviral immune responses by controlling the ubiquitination of RIG-I and MAVS.

  2. Design and Analysis of Tooth Impact Test Rig for Spur Gear

    NASA Astrophysics Data System (ADS)

    Ghazali, Wafiuddin Bin Md; Aziz, Ismail Ali Bin Abdul; Daing Idris, Daing Mohamad Nafiz Bin; Ismail, Nurazima Binti; Sofian, Azizul Helmi Bin

    2016-02-01

    This paper is about the design and analysis of a prototype of tooth impact test rig for spur gear. The test rig was fabricated and analysis was conducted to study its’ limitation and capabilities. The design of the rig is analysed to ensure that there will be no problem occurring during the test and reliable data can be obtained. From the result of the analysis, the maximum amount of load that can be applied, the factor of safety of the machine, the stresses on the test rig parts were determined. This is important in the design consideration of the test rig. The materials used for the fabrication of the test rig were also discussed and analysed. MSC Nastran Patran software was used to analyse the model, which was designed by using SolidWorks 2014 software. Based from the results, there were limitations found from the initial design and the test rig design needs to be improved in order for the test rig to operate properly.

  3. What Really Rigs Up RIG-I?

    PubMed

    Barik, Sailen

    2016-01-01

    RIG-I (retinoic acid-inducible gene 1) is an archetypal member of the cytoplasmic DEAD-box dsRNA helicase family (RIG-I-like receptors or RLRs), the members of which play essential roles in the innate immune response of the metazoan cell. RIG-I functions as a pattern recognition receptor that detects nonself RNA as a pathogen-associated molecular pattern (PAMP). However, the exact molecular nature of the viral RNAs that act as a RIG-I ligand has remained a mystery and a matter of debate. In this article, we offer a critical review of the actual viral RNAs that act as PAMPs to activate RIG-I, as seen from the perspective of a virologist, including a recent report that the viral Leader-read-through transcript is a novel and effective RIG-I ligand. © 2016 S. Karger AG, Basel.

  4. Ubiquitin-like modifier FAT10 attenuates RIG-I mediated antiviral signaling by segregating activated RIG-I from its signaling platform

    PubMed Central

    Nguyen, Nhung T.H.; Now, Hesung; Kim, Woo-Jong; Kim, Nari; Yoo, Joo-Yeon

    2016-01-01

    RIG-I is a key cytosolic RNA sensor that mediates innate immune defense against RNA virus. Aberrant RIG-I activity leads to severe pathological states such as autosomal dominant multi-system disorder, inflammatory myophathies and dermatomyositis. Therefore, identification of regulators that ensure efficient defense without harmful immune-pathology is particularly critical to deal with RIG-I-associated diseases. Here, we presented the inflammatory inducible FAT10 as a novel negative regulator of RIG-I-mediated inflammatory response. In various cell lines, FAT10 protein is undetectable unless it is induced by pro-inflammatory cytokines. FAT10 non-covalently associated with the 2CARD domain of RIG-I, and inhibited viral RNA-induced IRF3 and NF-kB activation through modulating the RIG-I protein solubility. We further demonstrated that FAT10 was recruited to RIG-I-TRIM25 to form an inhibitory complex where FAT10 was stabilized by E3 ligase TRIM25. As the result, FAT10 inhibited the antiviral stress granules formation contains RIG-I and sequestered the active RIG-I away from the mitochondria. Our study presented a novel mechanism to dampen RIG-I activity. Highly accumulated FAT10 is observed in various cancers with pro-inflammatory environment, therefore, our finding which uncovered the suppressive effect of the accumulated FAT10 during virus-mediated inflammatory response may also provide molecular clue to understand the carcinogenesis related with infection and inflammation. PMID:26996158

  5. Paramyxovirus V Proteins Interact with the RIG-I/TRIM25 Regulatory Complex and Inhibit RIG-I Signaling.

    PubMed

    Sánchez-Aparicio, Maria T; Feinman, Leighland J; García-Sastre, Adolfo; Shaw, Megan L

    2018-03-15

    Paramyxovirus V proteins are known antagonists of the RIG-I-like receptor (RLR)-mediated interferon induction pathway, interacting with and inhibiting the RLR MDA5. We report interactions between the Nipah virus V protein and both RIG-I regulatory protein TRIM25 and RIG-I. We also observed interactions between these host proteins and the V proteins of measles virus, Sendai virus, and parainfluenza virus. These interactions are mediated by the conserved C-terminal domain of the V protein, which binds to the tandem caspase activation and recruitment domains (CARDs) of RIG-I (the region of TRIM25 ubiquitination) and to the SPRY domain of TRIM25, which mediates TRIM25 interaction with the RIG-I CARDs. Furthermore, we show that V interaction with TRIM25 and RIG-I prevents TRIM25-mediated ubiquitination of RIG-I and disrupts downstream RIG-I signaling to the mitochondrial antiviral signaling protein. This is a novel mechanism for innate immune inhibition by paramyxovirus V proteins, distinct from other known V protein functions such as MDA5 and STAT1 antagonism. IMPORTANCE The host RIG-I signaling pathway is a key early obstacle to paramyxovirus infection, as it results in rapid induction of an antiviral response. This study shows that paramyxovirus V proteins interact with and inhibit the activation of RIG-I, thereby interrupting the antiviral signaling pathway and facilitating virus replication. Copyright © 2018 American Society for Microbiology.

  6. Ubiquitin-like modifier FAT10 attenuates RIG-I mediated antiviral signaling by segregating activated RIG-I from its signaling platform.

    PubMed

    Nguyen, Nhung T H; Now, Hesung; Kim, Woo-Jong; Kim, Nari; Yoo, Joo-Yeon

    2016-03-21

    RIG-I is a key cytosolic RNA sensor that mediates innate immune defense against RNA virus. Aberrant RIG-I activity leads to severe pathological states such as autosomal dominant multi-system disorder, inflammatory myophathies and dermatomyositis. Therefore, identification of regulators that ensure efficient defense without harmful immune-pathology is particularly critical to deal with RIG-I-associated diseases. Here, we presented the inflammatory inducible FAT10 as a novel negative regulator of RIG-I-mediated inflammatory response. In various cell lines, FAT10 protein is undetectable unless it is induced by pro-inflammatory cytokines. FAT10 non-covalently associated with the 2CARD domain of RIG-I, and inhibited viral RNA-induced IRF3 and NF-kB activation through modulating the RIG-I protein solubility. We further demonstrated that FAT10 was recruited to RIG-I-TRIM25 to form an inhibitory complex where FAT10 was stabilized by E3 ligase TRIM25. As the result, FAT10 inhibited the antiviral stress granules formation contains RIG-I and sequestered the active RIG-I away from the mitochondria. Our study presented a novel mechanism to dampen RIG-I activity. Highly accumulated FAT10 is observed in various cancers with pro-inflammatory environment, therefore, our finding which uncovered the suppressive effect of the accumulated FAT10 during virus-mediated inflammatory response may also provide molecular clue to understand the carcinogenesis related with infection and inflammation.

  7. Phosphorylation-mediated negative regulation of RIG-I antiviral activity.

    PubMed

    Gack, Michaela U; Nistal-Villán, Estanislao; Inn, Kyung-Soo; García-Sastre, Adolfo; Jung, Jae U

    2010-04-01

    Recognition of invading viruses by the host is elicited by cellular sensors which trigger signaling cascades that lead to type I interferon (IFN) gene expression. Retinoic acid-inducible gene I (RIG-I) has emerged as a key receptor for the detection of viral RNA in the cytosol, inducing IFN-mediated innate immune responses to limit viral replication through its interaction with MAVS (also called IPS-1, CARDIF, or VISA). Upon the recognition of viral RNA, the Lys-172 residue of RIG-I undergoes ubiquitination induced by tripartite motif protein 25 (TRIM25), an essential protein for antiviral signal transduction. Here we demonstrate that phosphorylation represents another regulatory mechanism for RIG-I-mediated antiviral activity. Using protein purification and mass spectrometry analysis, we identified three phosphorylation sites in the amino-terminal caspase recruitment domains (CARDs) of RIG-I. One of these residues, Thr-170, is located in close proximity to Lys-172, and we speculated that its phosphorylation may affect Lys-172 ubiquitination and functional activation of RIG-I. Indeed, a RIG-I mutant carrying a phosphomimetic Glu residue in place of Thr-170 loses TRIM25 binding, Lys-172 ubiquitination, MAVS binding, and downstream signaling ability. This suggests that phosphorylation of RIG-I at Thr-170 inhibits RIG-I-mediated antiviral signal transduction. Immunoblot analysis with a phospho-specific antibody showed that the phosphorylation of the RIG-I Thr-170 residue is present under normal conditions but rapidly declines upon viral infection. Our results indicate that Thr-170 phosphorylation and TRIM25-mediated Lys-172 ubiquitination of RIG-I functionally antagonize each other. While Thr-170 phosphorylation keeps RIG-I latent, Lys-172 ubiquitination enables RIG-I to form a stable complex with MAVS, thereby inducing IFN signal transduction.

  8. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hepworth, J.C.; Foss, M.M.

    The fifth Energy and Minerals Field Institute program for Washington, D.C. Congressional and Executive Aides was held during August 15-21, 1982. The five-and-one-half day program was conducted through Wyoming, Colorado and Utah and consisted of visits to: an R and D tertiary petroleum production facility; an historic oil field entering secondary production; a surface uranium mine; a petroleum exploration drilling rig; a surface coal mine; an air cooled, coal-fired power plant; an oil shale site; a geothermal-electrical generating facility; and open pit copper mine and associated smelter and refinery; a petroleum refinery and an oil shale semi-works retort. During themore » field program, participants had opportunities to view communities affected by these activities, such as Wright City and Gillette, Wyoming, Parachute, Colorado and Milford and Cedar City, Utah. Throughout the program, aides met with local, state and industry officials and citizen leaders during bus rides, meals and site visits.« less

  9. Druzhba feasibility study: Barsukov and Tarasov fields, 1995. Barsukov and Tarasov production histories western equipment. Export trade information

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1996-01-03

    The study, conducted by NEFT, was funded by the U.S. Trade and Development Agency. The report shows the results of a feasibility study conducted for the rehabilitation of oil wells in Baruskov and Tarasov fields. The objectives of the study include a plan for improving well and waterflood performance, and to determine materials and equipment needed. The report also covers capital and operating costs, as well as an evaluation of project economics based on Russian law. This is Volume 2 of the study containing Production Histories-Western Equipment. It is divided into the following sections: (1) Job Descriptions; (2) Barsukov Histories/Logs;more » (3) Tarasov Histories/Logs; (4) Taxes Paid by PNG; (5) Vendors Literature; (6) Rigs; (7) Liners; (8) Directional Drilling; (9) Mechanical Perforating; (10) Camps; (11) Pumps; (12) Fishing; (13) Downhole Oil/Water Separator; (14) Plastic.« less

  10. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity.

    PubMed

    Gack, Michaela U; Shin, Young C; Joo, Chul-Hyun; Urano, Tomohiko; Liang, Chengyu; Sun, Lijun; Takeuchi, Osamu; Akira, Shizuo; Chen, Zhijian; Inoue, Satoshi; Jung, Jae U

    2007-04-19

    Retinoic-acid-inducible gene-I (RIG-I; also called DDX58) is a cytosolic viral RNA receptor that interacts with MAVS (also called VISA, IPS-1 or Cardif) to induce type I interferon-mediated host protective innate immunity against viral infection. Furthermore, members of the tripartite motif (TRIM) protein family, which contain a cluster of a RING-finger domain, a B box/coiled-coil domain and a SPRY domain, are involved in various cellular processes, including cell proliferation and antiviral activity. Here we report that the amino-terminal caspase recruitment domains (CARDs) of RIG-I undergo robust ubiquitination induced by TRIM25 in mammalian cells. The carboxy-terminal SPRY domain of TRIM25 interacts with the N-terminal CARDs of RIG-I; this interaction effectively delivers the Lys 63-linked ubiquitin moiety to the N-terminal CARDs of RIG-I, resulting in a marked increase in RIG-I downstream signalling activity. The Lys 172 residue of RIG-I is critical for efficient TRIM25-mediated ubiquitination and for MAVS binding, as well as the ability of RIG-I to induce antiviral signal transduction. Furthermore, gene targeting demonstrates that TRIM25 is essential not only for RIG-I ubiquitination but also for RIG-I-mediated interferon- production and antiviral activity in response to RNA virus infection. Thus, we demonstrate that TRIM25 E3 ubiquitin ligase induces the Lys 63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity.

  11. Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses

    PubMed Central

    Liu, Wei; Li, Jing; Zheng, Weinan; Shang, Yingli; Zhao, Zhendong; Wang, Shanshan; Bi, Yuhai; Zhang, Shuang; Xu, Chongfeng; Duan, Ziyuan; Zhang, Lianfeng; Wang, Yue L; Jiang, Zhengfan; Liu, Wenjun; Sun, Lei

    2017-01-01

    RIG-I is a key cytosolic pattern recognition receptor that interacts with MAVS to induce type I interferons (IFNs) against RNA virus infection. In this study, we found that cyclophilin A (CypA), a peptidyl-prolyl cis/trans isomerase, functioned as a critical positive regulator of RIG-I-mediated antiviral immune responses. Deficiency of CypA impaired RIG-I-mediated type I IFN production and promoted viral replication in human cells and mice. Upon Sendai virus infection, CypA increased the interaction between RIG-I and its E3 ubiquitin ligase TRIM25, leading to enhanced TRIM25-mediated K63-linked ubiquitination of RIG-I that facilitated recruitment of RIG-I to MAVS. In addition, CypA and TRIM25 competitively interacted with MAVS, thereby inhibiting TRIM25-induced K48-linked ubiquitination of MAVS. Taken together, our findings reveal an essential role of CypA in boosting RIG-I-mediated antiviral immune responses by controlling the ubiquitination of RIG-I and MAVS. DOI: http://dx.doi.org/10.7554/eLife.24425.001 PMID:28594325

  12. Antiviral activity of human oligoadenylate synthetases-like (OASL) is mediated by enhancing retinoic acid-inducible gene I (RIG-I) signaling

    PubMed Central

    Zhu, Jianzhong; Zhang, Yugen; Ghosh, Arundhati; Cuevas, Rolando A.; Forero, Adriana; Dhar, Jayeeta; Ibsen, Mikkel Søes; Schmid-Burgk, Jonathan Leo; Schmidt, Tobias; Ganapathiraju, Madhavi K.; Fujita, Takashi; Hartmann, Rune; Barik, Sailen; Hornung, Veit; Coyne, Carolyn B.; Sarkar, Saumendra N.

    2014-01-01

    SUMMARY Virus infection is sensed in the cytoplasm by retinoic acid-inducible gene I (RIG-I, also known as DDX58), which requires RNA and polyubiquitin binding to induce type I interferon (IFN), and activate cellular innate immunity. We show that the human IFN-inducible oligoadenylate synthetases-like (OASL) protein had antiviral activity and mediated RIG-I activation by mimicking polyubiquitin. Loss of OASL expression reduced RIG-I signaling and enhanced virus replication in human cells. Conversely, OASL expression suppressed replication of a number of viruses in a RIG-I-dependent manner and enhanced RIG-I-mediated IFN induction. OASL interacted and colocalized with RIG-I, and through its C-terminal ubiquitin-like domain specifically enhanced RIG-I signaling. Bone marrow derived macrophages from mice deficient for Oasl2 showed that among the two mouse orthologs of human OASL; Oasl2 is functionally similar to human OASL. Our findings show a mechanism by which human OASL contributes to host antiviral responses by enhancing RIG-I activation. PMID:24931123

  13. Structural features of influenza A virus panhandle RNA enabling the activation of RIG-I independently of 5′-triphosphate

    PubMed Central

    Lee, Mi-Kyung; Kim, Hee-Eun; Park, Eun-Byeol; Lee, Janghyun; Kim, Ki-Hun; Lim, Kyungeun; Yum, Seoyun; Lee, Young-Hoon; Kang, Suk-Jo; Lee, Joon-Hwa; Choi, Byong-Seok

    2016-01-01

    Retinoic acid-inducible gene I (RIG-I) recognizes specific molecular patterns of viral RNAs for inducing type I interferon. The C-terminal domain (CTD) of RIG-I binds to double-stranded RNA (dsRNA) with the 5′-triphosphate (5′-PPP), which induces a conformational change in RIG-I to an active form. It has been suggested that RIG-I detects infection of influenza A virus by recognizing the 5′-triphosphorylated panhandle structure of the viral RNA genome. Influenza panhandle RNA has a unique structure with a sharp helical bending. In spite of extensive studies of how viral RNAs activate RIG-I, whether the structural elements of the influenza panhandle RNA confer the ability to activate RIG-I signaling has been poorly explored. Here, we investigated the dynamics of the influenza panhandle RNA in complex with RIG-I CTD using NMR spectroscopy and showed that the bending structure of the panhandle RNA negates the requirement of a 5′-PPP moiety for RIG-I activation. PMID:27288441

  14. Involvement of zebrafish RIG-I in NF-κB and IFN signaling pathways: insights into functional conservation of RIG-I in antiviral innate immunity.

    PubMed

    Nie, Li; Zhang, Ying-sheng; Dong, Wei-ren; Xiang, Li-xin; Shao, Jian-zhong

    2015-01-01

    The retinoic acid-inducible gene I (RIG-I) is a critical sensor for host recognition of RNA virus infection and initiation of antiviral signaling pathways in mammals. However, data on the occurrence and functions of this molecule in lower vertebrates are limited. In this study, we characterized an RIG-I homolog (DrRIG-I) from zebrafish. Structurally, this DrRIG-I shares a number of conserved functional domains/motifs with its mammalian counterparts, namely, caspase activation and recruitment domain, DExD/H box, a helicase domain, and a C-terminal domain. Functionally, stimulation with DrRIG-I CARD in zebrafish embryos significantly activated the NF-κB and IFN signaling pathways, leading to the expression of TNF-α, IL-8 and IFN-induced Mx, ISG15, and viperin. However, knockdown of TRIM25 (a pivotal activator for RIG-I receptors) significantly suppressed the induced activation of IFN signaling. Results suggested the functional conservation of RIG-I receptors in the NF-κB and IFN signaling pathways between teleosts and mammals, providing a perspective into the evolutionary history of RIG-I-mediated antiviral innate immunity. Copyright © 2014 Elsevier Ltd. All rights reserved.

  15. Gene expression profile after activation of RIG-I in 5'ppp-dsRNA challenged DF1.

    PubMed

    Chen, Yang; Xu, Qi; Li, Yang; Liu, Ran; Huang, Zhengyang; Wang, Bin; Chen, Guohong

    2016-12-01

    Retinoic acid inducible gene I (RIG-I) can recognize influenza viruses and evoke the innate immune response. RIG-I is absent in the chicken genome, but is conserved in the genome of ducks. Lack of RIG-I renders chickens more susceptible to avian influenza infection, and the clinical symptoms are more prominent than in other poultry. It is unknown whether introduction of duck RIG-I into chicken cells can establish the immunity as is seen in ducks and the role of RIG-I in established immunity is unknown. In this study, a chicken cell strain with stable expression of duRIG-I was established by lentiviral infection, giving DF1/LV5-RIG-I, and a control strain DF1/LV5 was established in parallel. To verify stable, high level expression of duRIG-I in DF1 cells, the levels of duRIG-I mRNA and protein were determined by real-time RT-PCR and Western blot, respectively. Further, 5'triphosphate double stranded RNA (5'ppp-dsRNA) was used to mimic an RNA virus infection and the infected DF1/LV5-RIG-I and DF1/LV5 cells were subjected to high-throughput RNA-sequencing, which yielded 193.46 M reads and 39.07 G bases. A total of 278 differentially expressed genes (DEGs), i.e., duRIG-I-mediated responsive genes, were identified by RNA-seq. Among the 278 genes, 120 DEGs are annotated in the KEGG database, and the most reliable KEGG pathways are likely to be the signaling pathways of RIG-I like receptors. Functional analysis by Gene ontology (GO) indicates that the functions of these DEGs are primarily related to Type I interferon (IFN) signaling, IFN-β-mediated cellular responses and up-regulation of the RIG-I signaling pathway. Based on the shared genes among different pathways, a network representing crosstalk between RIG-I and other signaling pathways was constructed using Cytoscape software. The network suggests that RIG-mediated pathway may crosstalk with the Jak-STAT signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway, ubiquitin-mediated proteolysis and MAPK signaling pathway during the transduction of antiviral signals. After screening, a group of key responsive genes in RIG-I-mediated signaling pathways, such as ISG12-2, Mx1, IFIT5, TRIM25, USP18, STAT1, STAT2, IRF1, IRF7 and IRF8, were tested for differential expression by real-time RT-PCR. In summary, by combining our results and the current literature, we propose a RIG-I-mediated signaling network in chickens. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Engineering geology studies in the National Petroleum Reserve, Alaska

    USGS Publications Warehouse

    Kachadoorian, Reuben; Crory, F.E.

    1984-01-01

    Engineering geology studies were conducted in direct support of the exploration program in the National Petroleum Reserve, Alaska. The studies included laboratory and field tests and observations to address design and construction problems of airfields, roads, drill pads and foundations, and to evaluate their actual performance. Permafrost containing large amounts of near surface ground ice as wedges, masses, and intergranular ice, required that all construction activity not disturb the thermal regime of the ground surface, which could lead to thaw of permafrost and ground subsidence. Summer activity, therefore was not allowable, yet the winter climate was so harsh that winter work was slow and inefficient. To allow summer operations at well sites planned for all year activity, it was necessary to adapt existing techniques for arctic construction and to devise new ones. The design and construction of facilities at the deep exploration wells at Inigok, Tunalik, and Lisburne posed the greatest challenge. These sites, requiring a year or more to drill, could only be attempted if continuous access to drilling and logistic supplies could be assured throughout the year, including the possibility of bringing in another drill rig, in the event of a blowout. Thus all-seasons airstrips were required at these wells. Sufficient quantities of local gravel were not readily available at the Inigok and Tunalik sites to construct the airstrips with the required 6 feet or more of gravel to prevent the underlying permafrost from thawing. Therefore, insulation was used to maintain the subbase of local sands in a continuously frozen state, which in turn was overlain by 15 inches of gravel or sandy gravel. Tests at the U.S. Army Waterways Experimental Station defined the minimum thickness of gravel required above the insulation to provide the desired bearing capacity for the C-130 type aircraft without crushing the insulation. Field testing also included the evaluation of another design option, using military landing mat underlain by insulation. Temperature recording devices were installed beneath the landing mat test sections, insulated runways, roads, drill pads, and reserve pits, to monitor the actual conditions and confirm the design assumptions. Investigations of thaw-settlement, erosion, and revegetation of all areas affected by construction were also conducted in anticipation of abandoning the sites, or, upgrading the facilities in the event the design life was extended.

  17. Energy Return on Investment (EROI) for Forty Global Oilfields Using a Detailed Engineering-Based Model of Oil Production.

    PubMed

    Brandt, Adam R; Sun, Yuchi; Bharadwaj, Sharad; Livingston, David; Tan, Eugene; Gordon, Deborah

    2015-01-01

    Studies of the energy return on investment (EROI) for oil production generally rely on aggregated statistics for large regions or countries. In order to better understand the drivers of the energy productivity of oil production, we use a novel approach that applies a detailed field-level engineering model of oil and gas production to estimate energy requirements of drilling, producing, processing, and transporting crude oil. We examine 40 global oilfields, utilizing detailed data for each field from hundreds of technical and scientific data sources. Resulting net energy return (NER) ratios for studied oil fields range from ≈2 to ≈100 MJ crude oil produced per MJ of total fuels consumed. External energy return (EER) ratios, which compare energy produced to energy consumed from external sources, exceed 1000:1 for fields that are largely self-sufficient. The lowest energy returns are found to come from thermally-enhanced oil recovery technologies. Results are generally insensitive to reasonable ranges of assumptions explored in sensitivity analysis. Fields with very large associated gas production are sensitive to assumptions about surface fluids processing due to the shifts in energy consumed under different gas treatment configurations. This model does not currently include energy invested in building oilfield capital equipment (e.g., drilling rigs), nor does it include other indirect energy uses such as labor or services.

  18. The AirWaterGas Teacher Professional Development Program: Lessons Learned by Pairing Scientists and Teachers to Develop Curriculum on Global Climate Change and Regional Unconventional Oil and Gas Development

    NASA Astrophysics Data System (ADS)

    Gardiner, L. S.; Hatheway, B.; Rogers, J. D.; Casey, J. G.; Lackey, G.; Birdsell, D.; Brown, K.; Polmear, M.; Capps, S.; Rosenblum, J.; Sitterley, K.; Hafich, K. A.; Hannigan, M.; Knight, D.

    2015-12-01

    The AirWaterGas Teacher Professional Development Program, run by the UCAR Center for Science Education, brought together scientists and secondary science teachers in a yearlong program culminating in the development of curriculum related to the impacts of unconventional oil and gas development. Graduate students and research scientists taught about their research area and its relationship to oil and gas throughout three online courses during the 2015-16 school year, during which teachers and scientists engaged in active online discussions. Topics covered included climate change, oil and gas infrastructure, air quality, water quality, public health, and practices and policies relating to oil and gas development. Building upon their initial online interactions and a face-to-face meeting in March, teachers were paired with appropriate AirWaterGas team members as science advisors during a month-long residency in Boulder, Colorado. During the residency, graduate student scientists provided resources and feedback as teachers developed curriculum projects in collaboration with each other and UCAR science educators. Additionally, teachers and AirWaterGas researchers shared experiences on an oil and gas well site tour, and a short course on drilling methods with a drilling rig simulator. Here, we share lessons learned from both sides of the aisle, including initial results from program assessment conducted with the participating teachers.

  19. Development of a two-body wet abrasion test method with attention to the effects of reused abradant

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Blau, Peter Julian; Dehoff, Ryan R

    2012-01-01

    Abrasive wear is among the most common and costliest causes for material wastage, and it occurs in many forms. A simple method has been developed to quantify the response of metals and alloys to two-body wet abrasion. A metallographic polishing machine was modified to create a disk-on-flat sliding test rig. Adhesive-backed SiC grinding papers were used under fixed load and speed to rank the abrasive wear of seven alloy steels, some of which are candidates for drill cones for geothermal drilling. Standardized two-body abrasion tests, like those described in ASTM G132, feed unused abrasive into the contact; however, the currentmore » work investigated whether useful rankings could still be obtained with a simpler testing configuration in which specimens repeatedly slide on the same wear path under water-lubricated conditions. Tests using abrasive grit sizes of 120 and 180 resulted in the same relative ranking of the alloys although the coarser grit produced more total wear. Wear decreased when the same abrasive disk was re-used for up to five runs, but the relative rankings of the steels remained the same. This procedure was presented to ASTM Committee G2 on Wear and Erosion as a potential standard test for wet two-body abrasive wear.« less

  20. Energy Return on Investment (EROI) for Forty Global Oilfields Using a Detailed Engineering-Based Model of Oil Production

    PubMed Central

    Brandt, Adam R.; Sun, Yuchi; Bharadwaj, Sharad; Livingston, David; Tan, Eugene; Gordon, Deborah

    2015-01-01

    Studies of the energy return on investment (EROI) for oil production generally rely on aggregated statistics for large regions or countries. In order to better understand the drivers of the energy productivity of oil production, we use a novel approach that applies a detailed field-level engineering model of oil and gas production to estimate energy requirements of drilling, producing, processing, and transporting crude oil. We examine 40 global oilfields, utilizing detailed data for each field from hundreds of technical and scientific data sources. Resulting net energy return (NER) ratios for studied oil fields range from ≈2 to ≈100 MJ crude oil produced per MJ of total fuels consumed. External energy return (EER) ratios, which compare energy produced to energy consumed from external sources, exceed 1000:1 for fields that are largely self-sufficient. The lowest energy returns are found to come from thermally-enhanced oil recovery technologies. Results are generally insensitive to reasonable ranges of assumptions explored in sensitivity analysis. Fields with very large associated gas production are sensitive to assumptions about surface fluids processing due to the shifts in energy consumed under different gas treatment configurations. This model does not currently include energy invested in building oilfield capital equipment (e.g., drilling rigs), nor does it include other indirect energy uses such as labor or services. PMID:26695068

  1. Mutual Regulation of NOD2 and RIG-I in Zebrafish Provides Insights into the Coordination between Innate Antibacterial and Antiviral Signaling Pathways.

    PubMed

    Nie, Li; Xu, Xiao-Xiao; Xiang, Li-Xin; Shao, Jian-Zhong; Chen, Jiong

    2017-05-27

    Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) and retinoic acid-inducible gene I (RIG-I) are two important cytosolic pattern recognition receptors (PRRs) in the recognition of pathogen-associated molecular patterns (PAMPs), initiating innate antibacterial and antiviral signaling pathways. However, the relationship between these PRRs, especially in teleost fish models, is rarely reported. In this article, we describe the mutual regulation of zebrafish NOD2 ( Dr NOD2) and RIG-I ( Dr RIG-I) in innate immune responses. Luciferase assays were conducted to determine the activation of NF-κB and interferon signaling. Morpholino-mediated knockdown and mRNA-mediated rescue were performed to further confirm the regulatory roles between Dr NOD2 and Dr RIG-I. Results showed that Dr NOD2 and Dr RIG-I shared conserved structural hallmarks with their mammalian counterparts, and activated Dr RIG-I signaling can induce Dr NOD2 production. Surprisingly, Dr NOD2-initiated signaling can also induce Dr RIG-I expression, indicating that a mutual regulatory mechanism may exist between them. Studies conducted using HEK293T cells and zebrafish embryos showed that Dr RIG-I could negatively regulate Dr NOD2-activated NF-κB signaling, and Dr NOD2 could inhibit Dr RIG-I-induced IFN signaling. Moreover, knocking down Dr RIG-I expression by morpholino could enhance Dr NOD2-initiated NF-κB activation, and vice versa, which could be rescued by their corresponding mRNAs. Results revealed a mutual feedback regulatory mechanism underlying NOD2 and RIG-I signaling pathways in teleosts. This mechanism reflects the coordination between cytosolic antibacterial and antiviral PRRs in the complex network of innate immunity.

  2. RIG-I overexpression decreases mortality of cigarette smoke exposed mice during influenza A virus infection.

    PubMed

    Wang, Xiaoqiu; Wu, Wenxin; Zhang, Wei; Leland Booth, J; Duggan, Elizabeth S; Tian, Lili; More, Sunil; Zhao, Yan D; Sawh, Ravindranauth N; Liu, Lin; Zou, Ming-Hui; Metcalf, Jordan P

    2017-09-02

    Retinoic acid-inducible gene I (RIG-I) is an important regulator of virus-induced antiviral interferons (IFNs) and proinflammatory cytokines which participate in clearing viral infections. Cigarette smoke (CS) exposure increases the frequency and severity of respiratory tract infections. We generated a RIG-I transgenic (TG) mouse strain that expresses the RIG-I gene product under the control of the human lung specific surfactant protein C promoter. We compared the mortality and host immune responses of RIG-I TG mice and their litter-matched wild type (WT) mice following challenge with influenza A virus (IAV). RIG-I overexpression increased survival of IAV-infected mice. CS exposure increased mortality in WT mice infected with IAV. Remarkably, the effect of RIG-I overexpression on survival during IAV infection was enhanced in CS-exposed animals. CS-exposed IAV-infected WT mice had a suppressed innate response profile in the lung compared to sham-exposed IAV-infected WT mice in terms of the protein concentration, total cell count and inflammatory cell composition in the bronchoalveolar lavage fluid. RIG-I overexpression restored the innate immune response in CS-exposed mice to that seen in sham-exposed WT mice during IAV infection, and is likely responsible for enhanced survival in RIG-I TG mice as restoration preceded death of the animals. Our results demonstrate that RIG-I overexpression in mice is protective for CS enhanced susceptibility of smokers to influenza infection, and that CS mediated RIG-I suppression may be partially responsible for the increased morbidity and mortality of the mice exposed to IAV. Thus, optimizing the RIG-I response may be an important treatment strategy for CS-enhanced lung infections, particularly those due to IAV.

  3. Performance Evaluation of a High Bandwidth Liquid Fuel Modulation Valve for Active Combustion Control

    NASA Technical Reports Server (NTRS)

    Saus, Joseph R.; DeLaat, John C.; Chang, Clarence T.; Vrnak, Daniel R.

    2012-01-01

    At the NASA Glenn Research Center, a characterization rig was designed and constructed for the purpose of evaluating high bandwidth liquid fuel modulation devices to determine their suitability for active combustion control research. Incorporated into the rig s design are features that approximate conditions similar to those that would be encountered by a candidate device if it were installed on an actual combustion research rig. The characterized dynamic performance measures obtained through testing in the rig are planned to be accurate indicators of expected performance in an actual combustion testing environment. To evaluate how well the characterization rig predicts fuel modulator dynamic performance, characterization rig data was compared with performance data for a fuel modulator candidate when the candidate was in operation during combustion testing. Specifically, the nominal and off-nominal performance data for a magnetostrictive-actuated proportional fuel modulation valve is described. Valve performance data were collected with the characterization rig configured to emulate two different combustion rig fuel feed systems. Fuel mass flows and pressures, fuel feed line lengths, and fuel injector orifice size was approximated in the characterization rig. Valve performance data were also collected with the valve modulating the fuel into the two combustor rigs. Comparison of the predicted and actual valve performance data show that when the valve is operated near its design condition the characterization rig can appropriately predict the installed performance of the valve. Improvements to the characterization rig and accompanying modeling activities are underway to more accurately predict performance, especially for the devices under development to modulate fuel into the much smaller fuel injectors anticipated in future lean-burning low-emissions aircraft engine combustors.

  4. Drilling Polar Oceans with the European Research Icebreaker AURORA BOREALIS: the IODP Context

    NASA Astrophysics Data System (ADS)

    Lembke-Jene, Lester; Wolff-Boenisch, Bonnie; Azzolini, Roberto; Thiede, Joern; Biebow, Nicole; Eldholm, Olav; Egerton, Paul

    2010-05-01

    Polar oceans are characterized by extreme environmental conditions for humans and materials, and have remained the least accessible regions to scientists of the IODP. DSDP and ODP have for long faced specific technical and logistical problems when attempting to drill in ice-covered polar deep-sea basins. The Arctic Ocean and large areas of the high-latitude Southern Ocean remained largely un-sampled by ODP and remain one of the major scientific and technological challenges for IODP. Drilling in these regions has been discussed and anticipated for decades and the scientific rationales are reflected in the science plans of the international Nansen Arctic Drilling Program (NAD) or the Arctic Program Planning Group (APPG) of ODP/IODP, amongst others. More recently, the rationale to investigate the polar oceans in a holistic approach has been outlined by workshops, leading to strategic assessments of the scientific potential and new drilling proposals. The European Polar Board took the initiative to develop a plan for a novel and dedicated research icebreaker with technical capabilities hitherto unrealised. This research icebreaker will enable autonomous operations in the central Arctic Ocean and the Southern Ocean, even during the severest ice conditions in the deep winter, serving all marine disciplines of polar research including scientific drilling: The European Research Icebreaker and Deep-Sea Drilling Vessel AURORA BOREALIS. AURORA BOREALIS is presently planned as a multi-purpose vessel. The ship can be deployed as a research icebreaker in all polar waters during any season of the year, as it shall meet the specifications of the highest ice-class attainable (IACS Polar Code 1) for icebreakers. During the times when it is not employed for drilling, it will operate as the most technically advanced multi-disciplinary research vessel in the Arctic or polar Southern Ocean. AURORA BOREALIS will be a "European scientific flagship facility" (fully open to non-European partners), a multidisciplinary platform for studies ranging from the sub-seafloor into the atmosphere. AURORA BOREALIS was planned for her role in deep-sea drilling in consultation with engineers and technical experts familiar with the program and the operation of these vessels. All techniques currently deployed on IODP expeditions can be implemented onboard the vessel under polar weather and ice conditions, including the full range of re-entry, casing and cementing, and instrumentation options and the entire suite of downhole logging tools. Due to sufficient laboratory space, a full analytical workflow can be easily established comparable to existing permanent platforms, including clean rooms, diverse scanning and logging or incubation facilities. While the vessel is equipped with a dedicated deep-sea drilling rig, other coring and drilling techniques can be employed if needed (e.g. Rockdrill, MEBO, large diameter Kasten cores). AURORA BOREALIS is fitted to operate a CALYPSO Piston Coring System in polar waters. Future mud-return systems under consideration and testing for IODP to provide controlled borehole conditions in difficult facies are compatible with the layout of AURORA BOREALIS. The berthing capacity of 120 personnel total (scientists, technical support and crew) allows to accommodate a sufficient number of science party members offshore. The present scientific implementation documents plan for about one polar scientific drilling expedition per year in a to-be-determined configuration. As the vessel is a multi-dsiciplinary platform, operations for the entire year are not dependant on drilling operations alone. While principal access to the vessel will be based on a competitive proposal review and evaluation system, the allocation of timeslots specifically for drilling would preferably be given over to IODP handling and planning systems in a cooperative mode using the strengths and capacitites of the future program. Depending on interests and needs of the scientific communities a preferential focus in non-drilling expedition planning could be established e.g. for dedicated geophysical pre-site survey works in areas inaccessible by other vessels to secure critical data needed for later drilling expeditions. Based on ongoing expert consultations, it is safe to assume that the average costs for an Arctic or polar drilling expedition will be considerably lower than with an otherwise necessary multi-ship setup based on modelled expedition scenarios and annual operational cost calculations. Still, AURORA BOREALIS shall provide substantially enhanced scientific, operational, personnel and technical capacities offshore.

  5. [Innate immune responses against viral infection and its suppression by viral proteins].

    PubMed

    Oshiumi, Hiroyuki; Matsumoto, Misako; Seya, Tsukasa

    2013-01-01

    Retinoic acid-inducible gene-I(RIG-I) is a cytoplasmic RNA helicase and a viral RNA sensor. RIG-I recognizes 5' triphosphate double-stranded RNA (dsRNA) and activates the IPS-1 adaptor molecule. The association of IPS-1 with RIG-I causes the formation of the prion-like structure of IPS-1. This structure is essential for activation of the signaling required for the induction of type I interferon (IFN), which possesses strong antiviral activity. Recent studies have revealed the novel factors involved in the RIG-I-dependent pathway. DDX3 and DDX60 RNA helicases associate with RIG-I and promote its binding to viral RNA. Riplet and TRIM25 ubiquitin ligase deliver Lys63-linked polyubiquitin moiety to RIG-I and result in signal activation. Several pathogenic viruses have evolved excellent systems to suppress type I IFN production. For example, NS3-4A of hepatitis C virus (HCV) cleaves IPS-1, which is the adaptor molecule of RIG-I, while the HCV core protein abrogates DDX3 function to suppress RIG-I-dependent IPS-1 activation, and the NS-1 of flu inhibits TRIM25 function to suppress RIG-I activation.

  6. Comparisons of Rig and Engine Dynamic Events in the Compressor of an Axi-Centrifugal Turboshaft Engine

    NASA Technical Reports Server (NTRS)

    Owen, A. Karl; Mattern, Duane L.; Le, Dzu K.

    1996-01-01

    Steady state and dynamic data were acquired in a T55-L-712 compressor rig. In addition, a T55-L-12 engine was instrumented and similar data were acquired. Rig and engine stall/surge data were analyzed using modal techniques. This paper compares rig and engine preliminary results for the ground idle (approximately 60% of design speed) point. The results of these analyses indicate both rig and engine dynamic event are preceded by indications of traveling wave energy in front of the compressor face. For both rig and engine, the traveling wave energy contains broad band energy with some prominent narrow peaks and, while the events are similar in many ways, some noticeable differences exist between the results of the analyses of rig data and engine data.

  7. Petroleum Development Oman gas exploration unlocks major new reserves

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wood, A.; Mozetic, A.

    1995-08-01

    Since 1985, Petroleum Development Oman (PDO) has been exploring for gas on behalf of the Government of Oman under a ten-year agreement signed in June 1984. The aim of the one-rig programme was to find additional non-associated gas reserves (3 TCF) to meet domestic energy requirements for a minimum of 40 years, for which the available reserves at that time (5.6 TCF) were insufficient. Initial results of the campaign, which principally targeted the Permian Khuff Formation, were disappointing, analogues to the major accumulations of the Arabian Gulf failing to materialise. During the second half of the programme, therefore, the strategymore » was revised to address the prospectivity of higher risk/higher reward plays recognised at greater depths. Well Saih Nihayda-24, drilled in 1989, found gas/condensate-bearing reservoirs in Cambro/Ordovician sandstones of the Andam Formation below 4000 metres. This discovery, in a seismically poorly defined anticline, sparked an intensive effort of 2D, and later 3D, long cable seismic acquisition. This led in 1991 to additional major gas/condensate finds in Saih Rawl and Barik, and a dedicated two-year two-rig appraisal campaign has since proven up sufficient reserves to support an LNG gas export scheme. The ten-year programme has more than tripled Oman`s non-associated gas expectation reserves to some 22 TCF, exceeding-the target more than five-fold. Significant potential for further gas discoveries identified in both North and South Oman provides encouragement for continued successful gas exploration in the future.« less

  8. AURORA BOREALIS - Development of a New Research Icebreaker with Drilling Capability

    NASA Astrophysics Data System (ADS)

    Thiede, J.; Biebow, N.; Egerton, P.; Kunz-Pirrung, M.; Lembke-Jene, L.

    2007-12-01

    Polar research both on land and in the sea cannot achieve the needed progress without novel and state of the art technologies and infrastructure. In addition, we have the obligation to equip the upcoming young and courageous generation of polar researchers with the most modern and safest research platforms the 21st century can provide. This effort will require major investments, both in terms of generating new tools, as well as maintaining and renovating existing infrastructure. There are many different novel tools under development for polar research, we will concentrate on the presently largest one, the planning for a new type of research icebreaker, the AURORA BOREALIS with an all-season capability of operations in permanently ice-covered waters and with the possibility to carry out deep-sea drilling in ice-covered deep-sea basins. AURORA BOREALIS will be the most advanced Polar Research Vessel in the world with a multi-functional role of drilling in deep ocean basins and supporting climate and environmental research and decision support for stakeholder governments for the next 35 to 40 years. The vessel is planned as a large research icebreaker with 44,000 tons displacement and a length of up to 196 m, with about 50 Megawatt propulsion power. Advanced technological features will include azimuth propulsion systems, extensive instrumental and airborne ice- management support, and the routine operation of Remotely Operated Vehicles (ROV) and Autonomous Underwater Vehicles (AUVs) from two moon-pools. An unique feature of this icebreaker will be the drilling rig that will enable sampling of the ocean floor and sub-sea down to 5000 m water depth and 1000 m penetration at the most inhospitable places on earth. The possibility to flexibly equip the ship with laboratory and supply containers, and the variable arrangement of other modular infrastructure (in particular, winches, cranes, etc.), free deck- space, and separate protected deck areas, will allow the planned research vessel to cover the needs of most disciplines in marine research. aurora-borealis.eu/en/about_aurora_borealis/

  9. Geologic Carbon Sequestration in a Lightly Explored Basin: the Puget-Willamette Lowland

    NASA Astrophysics Data System (ADS)

    Jackson, J. S.

    2007-12-01

    The Puget-Willamette Lowland is located between the Cascade Range and Olympic Mountains-Coast Range. Exploration for oil and gas there commenced in 1890. Over 700 wells subsequently drilled yield one commercial gas discovery. Eocene sediments deposited west of an ancestral Cascade Range include a coal-bearing sequence covering much of the Puget-Willamette Lowland. The terrestrial deposits pass into marine deposits to the west. Syn- depositional normal faulting and strike-slip faulting are evident in several sub-basins. In the southern Lowland, normal faults were modified by episodes of late Eocene and Miocene transpression, which resulted in mild inversion of older normal faults Preserved sediments indicate that local subsidence continued into Miocene- Pliocene time, and was followed in the northern Lowland by extensive Pleistocene glaciation. In the northern Lowland, Holocene faulting is recognized in outcrop and is interpreted on seismic data acquired in Puget Sound. Structures formed by early Miocene or earlier events may have trapped migrating hydrocarbons. Structures formed or modified by Holocene faulting very probably post-date hydrocarbon generation and migration. The region appears to host potential geologic sequestration targets, including coals, sandstones, and vesicular basalt flows. The size and location of potential traps is poorly constrained by present data. Experience in better explored fore arc basins suggests 10 to 30 percent of the basin may be deformed into suitable trapping geometries. Modern seismic data is required to identify potential sequestration traps. More than one well will be required to confirm the presence and size of these traps. The present boom in oil and gas drilling has created a robust environment for seismic and drilling companies, who command unprecedented rates for their services. Only one seismic crew is presently active on the West Coast, and only a few exploration drilling rigs are available. If this environment persists, then sequestration efforts will compete directly with the hydrocarbon industry for these services, leading to higher service company prices as well as delayed schedules. Carbon sequestration policy thus entails financial incentives that allow geologic sequestration projects to compete for exploration services.

  10. Viral RNA-Unprimed Rig-I Restrains Stat3 Activation in the Modulation of Regulatory T Cell/Th17 Cell Balance.

    PubMed

    Yang, Hui; Guo, He-Zhou; Li, Xian-Yang; Lin, Jian; Zhang, Wu; Zhao, Jun-Mei; Zhang, Hong-Xin; Chen, Sai-Juan; Chen, Zhu; Zhu, Jiang

    2017-07-01

    Innate immunity activation by viral RNA-primed retinoid acid inducible gene-I (Rig-I) in CD4 + T cells antagonizes TGFβ signaling to suppress the differentiation of regulatory T cells (Tregs). However, how viral RNA-unliganded Rig-I (apo-Rig-I) modulates Treg generation remains unclear. In this article, we show that, in the absence of viral infection, Treg differentiation of Rig-I -/- CD4 + T cells was compromised, in the presence of increased generation of Th17 cells and overactivation of Stat3, a critical regulator tilting the Treg/Th17 cell balance. Mechanistically, apo-Rig-I physically associates with Stat3, thereby inhibiting Jak1's association with Stat3 while facilitating Shp2's association to inhibit p-Stat3 levels. Interestingly, inhibition of Stat3 ameliorates the Treg/Th17 imbalance and the colitis observed in Rig-I -/- mice. Collectively, these results uncover an independent functional contribution of the apo-Rig-I/Stat3 interaction in the maintenance of Treg/Th17 cell balance. Copyright © 2017 by The American Association of Immunologists, Inc.

  11. Negative regulation of RIG-I-mediated antiviral signaling by TRK-fused gene (TFG) protein.

    PubMed

    Lee, Na-Rae; Shin, Han-Bo; Kim, Hye-In; Choi, Myung-Soo; Inn, Kyung-Soo

    2013-07-19

    RIG-I (retinoic acid inducible gene I)-mediated antiviral signaling serves as the first line of defense against viral infection. Upon detection of viral RNA, RIG-I undergoes TRIM25 (tripartite motif protein 25)-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that TRK-fused gene (TFG) protein, previously identified as a TRIM25-interacting protein, binds TRIM25 upon virus infection and negatively regulates RIG-I-mediated type-I IFN signaling. RIG-I-mediated IFN production and nuclear factor (NF)-κB signaling pathways were upregulated by the suppression of TFG expression. Furthermore, vesicular stomatitis virus (VSV) replication was significantly inhibited by small inhibitory hairpin RNA (shRNA)-mediated knockdown of TFG, supporting the suppressive role of TFG in RIG-I-mediated antiviral signaling. Interestingly, suppression of TFG expression increased not only RIG-I-mediated signaling but also MAVS (mitochondrial antiviral signaling protein)-induced signaling, suggesting that TFG plays a pivotal role in negative regulation of RNA-sensing, RIG-I-like receptor (RLR) family signaling pathways. Copyright © 2013 Elsevier Inc. All rights reserved.

  12. Rig-I regulates NF-κB activity through binding to Nf-κb1 3′-UTR mRNA

    PubMed Central

    Zhang, Hong-Xin; Liu, Zi-Xing; Sun, Yue-Ping; Lu, Shun-Yuan; Liu, Xue-Song; Huang, Qiu-Hua; Xie, Yin-Yin; Dang, Su-Ying; Zheng, Guang-Yong; Li, Yi-Xue; Kuang, Ying; Fei, Jian; Chen, Zhu; Wang, Zhu-Gang

    2013-01-01

    Retinoic acid inducible gene I (RIG-I) senses viral RNAs and triggers innate antiviral responses through induction of type I IFNs and inflammatory cytokines. However, whether RIG-I interacts with host cellular RNA remains undetermined. Here we report that Rig-I interacts with multiple cellular mRNAs, especially Nf-κb1. Rig-I is required for NF-κB activity via regulating Nf-κb1 expression at posttranscriptional levels. It interacts with the multiple binding sites within 3′-UTR of Nf-κb1 mRNA. Further analyses reveal that three distinct tandem motifs enriched in the 3′-UTR fragments can be recognized by Rig-I. The 3′-UTR binding with Rig-I plays a critical role in normal translation of Nf-κb1 by recruiting the ribosomal proteins [ribosomal protein L13 (Rpl13) and Rpl8] and rRNAs (18S and 28S). Down-regulation of Rig-I or Rpl13 significantly reduces Nf-κb1 and 3′-UTR–mediated luciferase expression levels. These findings indicate that Rig-I functions as a positive regulator for NF-κB signaling and is involved in multiple biological processes in addition to host antivirus immunity. PMID:23553835

  13. Activation of duck RIG-I by TRIM25 is independent of anchored ubiquitin.

    PubMed

    Miranzo-Navarro, Domingo; Magor, Katharine E

    2014-01-01

    Retinoic acid inducible gene I (RIG-I) is a viral RNA sensor crucial in defense against several viruses including measles, influenza A and hepatitis C. RIG-I activates type-I interferon signalling through the adaptor for mitochondrial antiviral signaling (MAVS). The E3 ubiquitin ligase, tripartite motif containing protein 25 (TRIM25), activates human RIG-I through generation of anchored K63-linked polyubiquitin chains attached to lysine 172, or alternatively, through the generation of unanchored K63-linked polyubiquitin chains that interact non-covalently with RIG-I CARD domains. Previously, we identified RIG-I of ducks, of interest because ducks are the host and natural reservoir of influenza viruses, and showed it initiates innate immune signaling leading to production of interferon-beta (IFN-β). We noted that K172 is not conserved in RIG-I of ducks and other avian species, or mouse. Because K172 is important for both mechanisms of activation of human RIG-I, we investigated whether duck RIG-I was activated by TRIM25, and if other residues were the sites for attachment of ubiquitin. Here we show duck RIG-I CARD domains are ubiquitinated for activation, and ubiquitination depends on interaction with TRIM25, as a splice variant that cannot interact with TRIM25 is not ubiquitinated, and cannot be activated. We expressed GST-fusion proteins of duck CARD domains and characterized TRIM25 modifications of CARD domains by mass spectrometry. We identified two sites that are ubiquitinated in duck CARD domains, K167 and K193, and detected K63 linked polyubiquitin chains. Site directed mutagenesis of each site alone, does not alter the ubiquitination profile of the duck CARD domains. However, mutation of both sites resulted in loss of all attached ubiquitin and polyubiquitin chains. Remarkably, the double mutant duck RIG-I CARD still interacts with TRIM25, and can still be activated. Our results demonstrate that anchored ubiquitin chains are not necessary for TRIM25 activation of duck RIG-I.

  14. Pigeon RIG-I Function in Innate Immunity against H9N2 IAV and IBDV.

    PubMed

    Xu, Wenping; Shao, Qiang; Zang, Yunlong; Guo, Qiang; Zhang, Yongchao; Li, Zandong

    2015-07-22

    Retinoic acid-inducible gene I (RIG-I), a cytosolic pattern recognition receptor (PRR), can sense various RNA viruses, including the avian influenza virus (AIV) and infectious bursal disease virus (IBDV), and trigger the innate immune response. Previous studies have shown that mammalian RIG-I (human and mice) and waterfowl RIG-I (ducks and geese) are essential for type I interferon (IFN) synthesis during AIV infection. Like ducks, pigeons are also susceptible to infection but are ineffective propagators and disseminators of AIVs, i.e., "dead end" hosts for AIVs and even highly pathogenic avian influenza (HPAI). Consequently, we sought to identify pigeon RIG-I and investigate its roles in the detection of A/Chicken/Shandong/ZB/2007 (H9N2) (ZB07), Gansu/Tianshui (IBDV TS) and Beijing/CJ/1980 (IBDV CJ-801) strains in chicken DF-1 fibroblasts or human 293T cells. Pigeon mRNA encoding the putative pigeon RIG-I analogs was identified. The exogenous expression of enhanced green fluorescence protein (EGFP)-tagged pigeon RIG-I and caspase activation and recruitment domains (CARDs), strongly induced antiviral gene (IFN-β, Mx, and PKR) mRNA synthesis, decreased viral gene (M gene and VP2) mRNA expression, and reduced the viral titers of ZB07 and IBDV TS/CJ-801 virus strains in chicken DF-1 cells, but not in 293T cells. We also compared the antiviral abilities of RIG-I proteins from waterfowl (duck and goose) and pigeon. Our data indicated that waterfowl RIG-I are more effective in the induction of antiviral genes and the repression of ZB07 and IBDV TS/CJ-801 strain replication than pigeon RIG-I. Furthermore, chicken melanoma differentiation associated gene 5(MDA5)/ mitochondrial antiviral signaling (MAVS) silencing combined with RIG-I transfection suggested that pigeon RIG-I can restore the antiviral response in MDA5-silenced DF-1 cells but not in MAVS-silenced DF-1 cells. In conclusion, these results demonstrated that pigeon RIG-I and CARDs have a strong antiviral ability against AIV H9N2 and IBDV in chicken DF-1 cells but not in human 293T cells.

  15. Hoisting and Rigging (Formerly Hoisting and Rigging Manual)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    NONE

    1995-06-01

    This standard is intended as a reference document to be used by supervisors, line managers, safety personnel, equipment operators, and any other personnel responsible for safety of hoisting and rigging operations at DOE sites. It quotes or paraphrases the US OSHA and ANSI requirements. It also encompasses, under one cover,hoisting and rigging requirements, codes, standards, and regulations, eliminating the need to maintain extensive (and often incomplete) libraries of hoisting and rigging standards throughout DOE. The standard occasionally goes beyond the minimum general industry standards established by OSHA and ANSI, and also delineates the more stringent requirements necessary to accomplish themore » complex, diversified, critical, and often hazardous hoisting and rigging work found with the DOE complex.« less

  16. Vapor Deposition Rig

    NASA Image and Video Library

    2015-01-27

    The Plasma Spray-Physical Vapor Deposition (PS-PVD) Rig at NASA Glenn Research Center. The rig helps develop coatings for next-generation aircraft turbine components and create more efficient engines.

  17. Trophic and reproductive ecology of Red Snapper, Lutjanus campechanus, on natural and artificial reefs in the western Gulf of Mexico

    NASA Astrophysics Data System (ADS)

    Ajemian, M. J.; Wetz, J. J.; Brewton, R. A.; Downey, C. H.; Stunz, G. W.

    2016-02-01

    Energy exploration in the Gulf of Mexico (Gulf) has resulted in the addition of numerous oil and gas production rigs that have added structurally complex habitat to an area otherwise dominated by bare bottom. The impact of these artificial structures on fish populations is largely unknown and there is ongoing debate about their functionality. Red Snapper (Lutjanus campechanus), an ecologically and economically important sportfish to the region, use natural as well as the artificial reefs created by standing and reefed (toppled or cutoff) oil and gas rigs. However, little is known about how trophic and reproductive characteristics of Red Snapper vary over these multiple habitat types. We analyzed stable isotopic composition (δ13C, δ 15N) of epaxial muscle and compared reproductive potential of Red Snapper (155-855 mm TL) from standing rigs, reefed rigs, and natural hard-bottom habitats off Texas. Red Snapper from standing rig sites were isotopically enriched in δ 15N compared to lower relief habitats, suggesting they were feeding at a higher trophic level on standing rigs. While gonadosomatic indices (GSI) and comparative histology implied a similar spawning season among structure types, GSI was highest for both sexes at standing rigs. These initial results suggest that while standing rigs appear to provide more enriched food resources leading to higher Red Snapper reproductive capacity, the productivity of this species is similar between currently permitted rig decommissioning options (i.e., cutoff and toppled rigs) and natural hard-bottom habitats in the Gulf of Mexico.

  18. The development of cold-water coral mounds along the Moroccan Atlantic and Mediterranean margins revealed by MeBo drillings

    NASA Astrophysics Data System (ADS)

    Hebbeln, Dierk; Wienberg, Claudia; Frank, Norbert

    2015-04-01

    Cold-water corals (CWC) mostly occur in intermediate water depths between 200 m and 1000 m and are capable of forming substantial seafloor structures, so-called coral carbonate mounds. These mounds can reach heights from a few meters up to >300 m and are composed of a mixture of CWC (and other shell) fragments and hemipelagic sediments, that both individually serve as distinct paleo-archives. IODP Leg 307 drilled through Challenger Mound at the Irish margin and revealed for the first time the full life history of a coral mound. However, although CWC occur almost worldwide, the 155 m long Challenger Mound record was for many years the only record from a coral mound exceeding 10 m in length. During expedition MSM36 with the German R/V MARIA S. MERIAN in spring 2014, several coral mounds along the Moroccan margin, both in the Atlantic Ocean and in the Mediterranean Sea, were drilled (actually: push-cored) by applying the Bremen Seafloor Drill Rig MeBo. The MeBo is a remotely controlled drilling system that is lowered from the vessel to the seafloor. Energy supply and video control are secured by an umbilical linking the MeBo to the vessel. The scientific foci of expedition MSM36 were to investigate (1) the long-term development of CWC mounds in both areas over the last several 100,000 years in relation to changes in the ambient environmental conditions in the respective intermediate waters, (2) the life time history of these mounds, and (3) the forcing factors for the initiation and decease of individual mounds. In both working areas, a total amount of 11 sites were successfully drilled with MeBo. Eight drillings were conducted at CWC mounds (on-mound sites) and 3 drillings in the direct vicinity of the mounds (off-mound sites) in order to obtain continuous paleoceanographic records. Drilling depths ranged between 17 m and 71 m with the latter corresponding to the maximum drilling depth of MeBo. The core recoveries varied between the sites and ranged between 47% and 96%. The coral-bearing on-mound cores were frozen and opened (i.e., cut lengthwise) with a stone saw to avoid a destruction of the original sediment texture with the embedded coral fragments. After opening, it became obvious that the quality of the MeBo cores is excellent and that it allows detailed post-cruise analyses at the MARUM laboratories in Bremen. By obtaining on-mound records reaching lengths of >70 m (focus #1), supplemented by the full penetration of three coral mounds (foci #2 and #3) and by a >45-m-long double drilling at an off-mound site located between numerous fossil and buried mounds (allowing to put their full life history into a wider paleoceanographic context; foci #1 to #3), the major technical goals of this MeBo expedition were fully accomplished. The critical factor in applying MeBo is the sea state as during deployment and recovery dynamic loads on the umbilical might reach critical limits. Although during expedition MSM36 several MeBo deployments were done by wind speeds of 6 Bft, the sea state especially in the Mediterranean Sea allowed MeBo operations without any restrictions. On the Atlantic side, a high swell, which actually exceeded the operational limit given for secured MeBo operations, could be overcome by reducing the payload (i.e. reducing the maximum drill depth). Hence, the operational window could be widened allowing for almost continuous MeBo operations also in this area.

  19. Negative regulation of RIG-I-mediated antiviral signaling by TRK-fused gene (TFG) protein

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lee, Na-Rae; Shin, Han-Bo; Kim, Hye-In

    2013-07-19

    Highlights: •TRK-fused gene product (TFG) interacts with TRIM25 upon viral infection. •TFG negatively regulates RIG-I mediated antiviral signaling. •TFG depletion leads to enhanced viral replication. •TFG act downstream of MAVS. -- Abstract: RIG-I (retinoic acid inducible gene I)-mediated antiviral signaling serves as the first line of defense against viral infection. Upon detection of viral RNA, RIG-I undergoes TRIM25 (tripartite motif protein 25)-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that TRK-fused gene (TFG) protein, previously identified as a TRIM25-interacting protein, binds TRIM25 upon virus infection and negatively regulates RIG-I-mediated type-I IFN signaling. RIG-I-mediatedmore » IFN production and nuclear factor (NF)-κB signaling pathways were upregulated by the suppression of TFG expression. Furthermore, vesicular stomatitis virus (VSV) replication was significantly inhibited by small inhibitory hairpin RNA (shRNA)-mediated knockdown of TFG, supporting the suppressive role of TFG in RIG-I-mediated antiviral signaling. Interestingly, suppression of TFG expression increased not only RIG-I-mediated signaling but also MAVS (mitochondrial antiviral signaling protein)-induced signaling, suggesting that TFG plays a pivotal role in negative regulation of RNA-sensing, RIG-I-like receptor (RLR) family signaling pathways.« less

  20. Hepatitis B virus inhibits intrinsic RIG-I and RIG-G immune signaling via inducing miR146a

    PubMed Central

    Hou, Zhaohua; Zhang, Jian; Han, Qiuju; Su, Chenhe; Qu, Jing; Xu, Dongqing; Zhang, Cai; Tian, Zhigang

    2016-01-01

    Previous studies showed that hepatitis B virus (HBV), as a latency invader, attenuated host anti-viral immune responses. miRNAs were shown to be involved in HBV infection and HBV-related diseases, however, the precise role of miRNAs in HBV-mediated immunosuppression remains unclear. Here, we observed that down-regulated RIG-I like receptors might be one critical mechanism of HBV-induced suppression of type I IFN transcription in both HBV+ hepatoma cell lines and liver cancer tissues. Then, miR146a was demonstrated to negatively regulate the expression of RIG-I-like receptors by directly targeting both RIG-I and RIG-G. Further investigation showed that antagonizing miR146a by anti-sense inhibitors or sponge approach accelerated HBV clearance and reduced HBV load both in vitro and in a HBV-carrying mouse model. Therefore, our findings indicated that HBV-induced miR146a attenuates cell-intrinsic anti-viral innate immunity through targeting RIG-I and RIG-G, and silencing miR146a might be an effective target to reverse HBV-induced immune suppression. PMID:27210312

  1. A calibration rig for multi-component internal strain gauge balance using the new design-of-experiment (DOE) approach

    NASA Astrophysics Data System (ADS)

    Nouri, N. M.; Mostafapour, K.; Kamran, M.

    2018-02-01

    In a closed water-tunnel circuit, the multi-component strain gauge force and moment sensor (also known as balance) are generally used to measure hydrodynamic forces and moments acting on scaled models. These balances are periodically calibrated by static loading. Their performance and accuracy depend significantly on the rig and the method of calibration. In this research, a new calibration rig was designed and constructed to calibrate multi-component internal strain gauge balances. The calibration rig has six degrees of freedom and six different component-loading structures that can be applied separately and synchronously. The system was designed based on the applicability of formal experimental design techniques, using gravity for balance loading and balance positioning and alignment relative to gravity. To evaluate the calibration rig, a six-component internal balance developed by Iran University of Science and Technology was calibrated using response surface methodology. According to the results, calibration rig met all design criteria. This rig provides the means by which various methods of formal experimental design techniques can be implemented. The simplicity of the rig saves time and money in the design of experiments and in balance calibration while simultaneously increasing the accuracy of these activities.

  2. RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease.

    PubMed

    Zhu, Huiyuan; Lou, Fangzhou; Yin, Qianqian; Gao, Yuanyuan; Sun, Yang; Bai, Jing; Xu, Zhenyao; Liu, Zhaoyuan; Cai, Wei; Ke, Fang; Zhang, Lingyun; Zhou, Hong; Wang, Hong; Wang, Gang; Chen, Xiang; Zhang, Hongxin; Wang, Zhugang; Ginhoux, Florent; Lu, Chuanjian; Su, Bing; Wang, Honglin

    2017-05-01

    Retinoic acid inducible-gene I (RIG-I) functions as one of the major sensors of RNA viruses. DDX58 , which encodes the RIG-I protein, has been newly identified as a susceptibility gene in psoriasis. Here, we show that the activation of RIG-I by 5'ppp-dsRNA, its synthetic ligand, directly causes the production of IL-23 and triggers psoriasis-like skin disease in mice. Repeated injections of IL-23 to the ears failed to induce IL-23 production and a full psoriasis-like skin phenotype, in either germ-free or RIG-I-deficient mice. RIG-I is also critical for a full development of skin inflammation in imiquimod (IMQ)-induced psoriasis-like mouse model. Furthermore, RIG-I-mediated endogenous IL-23 production was mainly confined to the CD11c + dendritic cells (DCs) via nuclear factor-kappa B (NF-κB) signaling, and stimulated RIG-I expression in an auto-regulatory feedback loop. Thus, our data suggest that the dysregulation in the antiviral immune responses of hosts through the innate pattern recognition receptors may trigger the skin inflammatory conditions in the pathophysiology of psoriasis. © 2017 The Authors. Published under the terms of the CC BY 4.0 license.

  3. Material Evaluation of an Elastomer, Epoxy and Lightweight Concrete Rail Attachment System for Direct Fixation Light Rail Applications

    NASA Astrophysics Data System (ADS)

    Swarner, Benjamin R.

    Sound Transit plans to extend its current light rail system, which runs along the I-5 corridor in Seattle, Washington, across the I-90 Homer Hadley floating bridge as part of a project to connect the major city centers in the region. But, no light rail has ever crossed a floating bridge due to several unique engineering challenges. One of these challenges is attaching the rails to the existing bridge deck without drilling into the bridge pontoons. This research program was developed to test and analyze a direct fixation method that uses lightweight concrete plinths and an elastomer-epoxy system to attach the rails to the bridge deck. The elastomer used was a two-part, pourable elastomer with cork particles intermixed to alter the mechanical properties of the material. A lightweight concrete mixture was analyzed for use in the plinths, and system tests investigated the system response under tensile, compressive and shear loading. The shear response of the system was examined further under varying loading conditions including different surface preparations, elastomer thicknesses, strain-rates and after freeze-thaw conditioning. Experimental data was examined for trends based on these parameters to best characterize the system, and the elastomer was evaluated in the context of modern elastomer research.

  4. Wind tunnel validation of AeroDyn within LIFES50+ project: imposed Surge and Pitch tests

    NASA Astrophysics Data System (ADS)

    Bayati, I.; Belloli, M.; Bernini, L.; Zasso, A.

    2016-09-01

    This paper presents the first set of results of the steady and unsteady wind tunnel tests, performed at Politecnico di Milano wind tunnel, on a 1/75 rigid scale model of the DTU 10 MW wind turbine, within the LIFES50+ project. The aim of these tests is the validation of the open source code AeroDyn developed at NREL. Numerical and experimental steady results are compared in terms of thrust and torque coefficients, showing good agreement, as well as for unsteady measurements gathered with a 2 degree-of-freedom test rig, capable of imposing the displacements at the base of the model, and providing the surge and pitch motion of the floating offshore wind turbine (FOWT) scale model. The measurements of the unsteady test configuration are compared with AeroDyn/Dynin module results, implementing the generalized dynamic wake (GDW) model. Numerical and experimental comparison showed similar behaviours in terms of non linear hysteresis, however some discrepancies are herein reported and need further data analysis and interpretations about the aerodynamic integral quantities, with a special attention to the physics of the unsteady phenomenon.

  5. Subcellular Localizations of RIG-I, TRIM25, and MAVS Complexes

    PubMed Central

    Sánchez-Aparicio, M. T.; Ayllón, J.; Leo-Macias, A.; Wolff, T.

    2016-01-01

    ABSTRACT The retinoic acid-inducible gene 1 (RIG-I) signaling pathway is essential for the recognition of viruses and the initiation of host interferon (IFN)-mediated antiviral responses. Once activated, RIG-I interacts with polyubiquitin chains generated by TRIM25 and binds mitochondrial antiviral signaling protein (MAVS), leading to the production of type I IFN. We now show specific interactions among these key partners in the RLR pathway through the use of bimolecular fluorescence complementation (BiFC) and super-resolution microscopy. Dimers of RIG-I, TRIM25, and MAVS localize into different compartments. Upon activation, we show that TRIM25 is redistributed into cytoplasmic dots associated with stress granules, while RIG-I associates with TRIM25/stress granules and with mitochondrial MAVS. In addition, MAVS competes with TRIM25 for RIG-I binding, and this suggests that upon TRIM25-mediated activation of RIG-I, RIG-I moves away from TRIM25 to interact with MAVS at the mitochondria. For the first time, the distribution of these three proteins was analyzed at the same time in virus-infected cells. We also investigated how specific viral proteins modify some of the protein complexes in the pathway. The protease NS3/4A from hepatitis C virus redistributes the complexes RIG-I/MAVS and MAVS/MAVS but not RIG-I/TRIM25. In contrast, the influenza A virus NS1 protein interacts with RIG-I and TRIM25 in specific areas in the cell cytoplasm and inhibits the formation of TRIM25 homocomplexes but not the formation of RIG-I/TRIM25 heterocomplexes, preventing the formation of RIG-I/MAVS complexes. Thus, we have localized spatially in the cell different complexes formed between RIG-I, TRIM25, and MAVS, in the presence or absence of two viral IFN antagonistic proteins. IMPORTANCE The first line of defense against viral infections is the innate immune response. Viruses are recognized by pathogen recognition receptors, such as the RIG-I like receptor family, that activate a signaling cascade that induces IFN production. In the present study, we visualized, for the first time in cells, both in overexpression and endogenous levels, complexes formed among key proteins involved in this innate immune signaling pathway. Through different techniques we were able to analyze how these proteins are distributed and reorganized spatially within the cell in order to transmit the signal, leading to an efficient antiviral state. In addition, this work presents a new means by how, when, and where viral proteins can target these pathways and act against the host immune system in order to counteract the activation of the immune response. PMID:27807226

  6. Subcellular Localizations of RIG-I, TRIM25, and MAVS Complexes.

    PubMed

    Sánchez-Aparicio, M T; Ayllón, J; Leo-Macias, A; Wolff, T; García-Sastre, A

    2017-01-15

    The retinoic acid-inducible gene 1 (RIG-I) signaling pathway is essential for the recognition of viruses and the initiation of host interferon (IFN)-mediated antiviral responses. Once activated, RIG-I interacts with polyubiquitin chains generated by TRIM25 and binds mitochondrial antiviral signaling protein (MAVS), leading to the production of type I IFN. We now show specific interactions among these key partners in the RLR pathway through the use of bimolecular fluorescence complementation (BiFC) and super-resolution microscopy. Dimers of RIG-I, TRIM25, and MAVS localize into different compartments. Upon activation, we show that TRIM25 is redistributed into cytoplasmic dots associated with stress granules, while RIG-I associates with TRIM25/stress granules and with mitochondrial MAVS. In addition, MAVS competes with TRIM25 for RIG-I binding, and this suggests that upon TRIM25-mediated activation of RIG-I, RIG-I moves away from TRIM25 to interact with MAVS at the mitochondria. For the first time, the distribution of these three proteins was analyzed at the same time in virus-infected cells. We also investigated how specific viral proteins modify some of the protein complexes in the pathway. The protease NS3/4A from hepatitis C virus redistributes the complexes RIG-I/MAVS and MAVS/MAVS but not RIG-I/TRIM25. In contrast, the influenza A virus NS1 protein interacts with RIG-I and TRIM25 in specific areas in the cell cytoplasm and inhibits the formation of TRIM25 homocomplexes but not the formation of RIG-I/TRIM25 heterocomplexes, preventing the formation of RIG-I/MAVS complexes. Thus, we have localized spatially in the cell different complexes formed between RIG-I, TRIM25, and MAVS, in the presence or absence of two viral IFN antagonistic proteins. The first line of defense against viral infections is the innate immune response. Viruses are recognized by pathogen recognition receptors, such as the RIG-I like receptor family, that activate a signaling cascade that induces IFN production. In the present study, we visualized, for the first time in cells, both in overexpression and endogenous levels, complexes formed among key proteins involved in this innate immune signaling pathway. Through different techniques we were able to analyze how these proteins are distributed and reorganized spatially within the cell in order to transmit the signal, leading to an efficient antiviral state. In addition, this work presents a new means by how, when, and where viral proteins can target these pathways and act against the host immune system in order to counteract the activation of the immune response. Copyright © 2017 American Society for Microbiology.

  7. Engine-Scale Combustor Rig Designed, Fabricated, and Tested for Combustion Instability Control Research

    NASA Technical Reports Server (NTRS)

    DeLaat, John C.; Breisacher, Kevin J.

    2000-01-01

    Low-emission combustor designs are prone to combustor instabilities. Because active control of these instabilities may allow future combustors to meet both stringent emissions and performance requirements, an experimental combustor rig was developed for investigating methods of actively suppressing combustion instabilities. The experimental rig has features similar to a real engine combustor and exhibits instabilities representative of those in aircraft gas turbine engines. Experimental testing in the spring of 1999 demonstrated that the rig can be tuned to closely represent an instability observed in engine tests. Future plans are to develop and demonstrate combustion instability control using this experimental combustor rig. The NASA Glenn Research Center at Lewis Field is leading the Combustion Instability Control program to investigate methods for actively suppressing combustion instabilities. Under this program, a single-nozzle, liquid-fueled research combustor rig was designed, fabricated, and tested. The rig has many of the complexities of a real engine combustor, including an actual fuel nozzle and swirler, dilution cooling, and an effusion-cooled liner. Prior to designing the experimental rig, a survey of aircraft engine combustion instability experience identified an instability observed in a prototype engine as a suitable candidate for replication. The frequency of the instability was 525 Hz, with an amplitude of approximately 1.5-psi peak-to-peak at a burner pressure of 200 psia. The single-nozzle experimental combustor rig was designed to preserve subcomponent lengths, cross sectional area distribution, flow distribution, pressure-drop distribution, temperature distribution, and other factors previously found to be determinants of burner acoustic frequencies, mode shapes, gain, and damping. Analytical models were used to predict the acoustic resonances of both the engine combustor and proposed experiment. The analysis confirmed that the test rig configuration and engine configuration had similar longitudinal acoustic characteristics, increasing the likelihood that the engine instability would be replicated in the rig. Parametric analytical studies were performed to understand the influence of geometry and condition variations and to establish a combustion test plan. Cold-flow experiments verified that the design values of area and flow distributions were obtained. Combustion test results established the existence of a longitudinal combustion instability in the 500-Hz range with a measured amplitude approximating that observed in the engine. Modifications to the rig configuration during testing also showed the potential for injector independence. The research combustor rig was developed in partnership with Pratt & Whitney of West Palm Beach, Florida, and United Technologies Research Center of East Hartford, Connecticut. Experimental testing of the combustor rig took place at United Technologies Research Center.

  8. Function of duck RIG-I in induction of antiviral response against IBDV and avian influenza virus on chicken cells.

    PubMed

    Shao, Qiang; Xu, Wenpin; Yan, Li; Liu, Jinhua; Rui, Lei; Xiao, Xiao; Yu, Xiaoxue; Lu, Yanan; Li, Zandong

    2014-10-13

    The avian influenza (AI) H9N2 virus and IBDV are two major problems in the poultry industry. They have been prevalent among domestic poultry in Asia for many years and have caused considerable economic losses. Retinoic-acid-induced gene I (RIG-I) is a cytoplasmic sensor of dsRNA and ssRNA. It can detect Encephalomyocarditis virus (EMCV) and vesicular stomatitis virus (VSV) in human cells, influenza virus in duck leads to production of IFN-β and IFN-stimulated antiviral genes and reductions in the replication of RNA virus. Chickens, which lack RIG-I, are more sensitive to influenza virus than ducks. However, little is known about the roles of duck RIG-I (dRIG-I) in the detection of IBDV and AI H9N2 in chicken cells DF-1. The purpose of this study was to examine the function of dRIG-I in the recognition of IBDV Ts strain and H9N2 A/Chicken/Shandong/ZB/2007(ZB07) and in the induction of antiviral gene expression to gain an understanding of antiviral ability of dRIG-I in chicken cells against dsRNA virus IBDV and ssRNA virus ZB07. After challenge with the IBDV Ts strain and ZB07 the expression levels of Type I IFN (IFN-β and IFN-α) and IFN-induced antiviral genes (Mx and PKR) were significantly up-regulated in dRIG-I-transfected DF-1cells compared with the empty-vector-transfected control. dRIG-I knockdown experiments further proved that dRIG-I is essential to sensing IBDV and ZB07 in duck embryo fibroblasts (DEF). Growth curves showed that dRIG-I repressed the replication of IBDV and almost blunted the growth of ZB07 in DF-1. Apoptosis analysis revealed that dRIG-I increase the number of the survival cells after IBDV Ts strain or ZB07 infection relative to the empty-vector-transfected control. These results indicate that dRIG-I can up-regulates type I IFN and reduce viral gene expression and viral replication and protect chicken cells from virus-induced apoptosis during ZB07 and IBDV infection. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. 46 CFR 162.050-17 - Separator test rig.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

  10. 46 CFR 162.050-17 - Separator test rig.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

  11. 46 CFR 162.050-17 - Separator test rig.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

  12. 46 CFR 162.050-17 - Separator test rig.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

  13. 46 CFR 162.050-17 - Separator test rig.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... diagram of a typical test rig is shown in Figure 162.050-17(a). FIGURE 162.050-17(a)—SEPARATOR TEST RIG... discharge side. (c) The inlet piping of the test rig must be sized so that— (1) Influent water flows at a Reynolds Number of at least 10,000; (2) The influent flow rate is between one and three meters per second...

  14. Robust Lys63-Linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection

    PubMed Central

    Jiang, Jingwen; Fan, Wenhui; Zheng, Weinan; Yu, Meng; Chen, Can; Sun, Lei; Bi, Yuhai; Ding, Chan; Gao, George F.

    2016-01-01

    ABSTRACT Influenza A and B virus infections both cause a host innate immunity response. Here, we report that the robust production of type I and III interferons (IFNs), IFN-stimulated genes, and proinflammatory factors can be induced by influenza B virus rather than influenza A virus infection in alveolar epithelial (A549) cells during early infection. This response is mainly dependent on the retinoic acid-inducible gene I (RIG-I)-mediated signaling pathway. Infection by influenza B virus promotes intense Lys63-linked ubiquitination of RIG-I, resulting in cytokine eruption. It is known that the influenza A virus NS1 protein (NS1-A) interacts with RIG-I and TRIM25 to suppress the activation of RIG-I-mediated signaling. However, the present results indicate that the influenza B virus NS1 protein (NS1-B) is unable to interact with RIG-I but engages in the formation of a RIG-I/TRIM25/NS1-B ternary complex. Furthermore, we demonstrate that the N-terminal RNA-binding domain (RBD) of NS1-B is responsible for interaction with TRIM25 and that this interaction blocks the inhibitory effect of the NS1-B C-terminal effector domain (TED) on RIG-I ubiquitination. Our findings reveal a novel mechanism for the host cytokine response to influenza B virus infection through regulatory interplay between host and viral proteins. IMPORTANCE Influenza B virus generally causes local mild epidemics but is occasionally lethal to individuals. Existing studies describe the broad characteristics of influenza B virus epidemiology and pathology. However, to develop better prevention and treatments for the disease, determining the concrete molecular mechanisms of pathogenesis becomes pivotal to understand how the host reacts to the challenge of influenza B virus. Thus, we aimed to characterize the host innate immune response to influenza B virus infection. Here, we show that vigorous Lys63-linked ubiquitination of RIG-I and cytokine eruption dependent on RIG-I-mediated signal transduction are induced by virus infection. Additionally, TRIM25 positively regulates RIG-I-mediated signaling by ablating the inhibitory function of NS1-B on RIG-I ubiquitination. PMID:27122586

  15. Activation of Duck RIG-I by TRIM25 Is Independent of Anchored Ubiquitin

    PubMed Central

    Miranzo-Navarro, Domingo; Magor, Katharine E.

    2014-01-01

    Retinoic acid inducible gene I (RIG-I) is a viral RNA sensor crucial in defense against several viruses including measles, influenza A and hepatitis C. RIG-I activates type-I interferon signalling through the adaptor for mitochondrial antiviral signaling (MAVS). The E3 ubiquitin ligase, tripartite motif containing protein 25 (TRIM25), activates human RIG-I through generation of anchored K63-linked polyubiquitin chains attached to lysine 172, or alternatively, through the generation of unanchored K63-linked polyubiquitin chains that interact non-covalently with RIG-I CARD domains. Previously, we identified RIG-I of ducks, of interest because ducks are the host and natural reservoir of influenza viruses, and showed it initiates innate immune signaling leading to production of interferon-beta (IFN-β). We noted that K172 is not conserved in RIG-I of ducks and other avian species, or mouse. Because K172 is important for both mechanisms of activation of human RIG-I, we investigated whether duck RIG-I was activated by TRIM25, and if other residues were the sites for attachment of ubiquitin. Here we show duck RIG-I CARD domains are ubiquitinated for activation, and ubiquitination depends on interaction with TRIM25, as a splice variant that cannot interact with TRIM25 is not ubiquitinated, and cannot be activated. We expressed GST-fusion proteins of duck CARD domains and characterized TRIM25 modifications of CARD domains by mass spectrometry. We identified two sites that are ubiquitinated in duck CARD domains, K167 and K193, and detected K63 linked polyubiquitin chains. Site directed mutagenesis of each site alone, does not alter the ubiquitination profile of the duck CARD domains. However, mutation of both sites resulted in loss of all attached ubiquitin and polyubiquitin chains. Remarkably, the double mutant duck RIG-I CARD still interacts with TRIM25, and can still be activated. Our results demonstrate that anchored ubiquitin chains are not necessary for TRIM25 activation of duck RIG-I. PMID:24466302

  16. Expression levels of the innate response gene RIG-I and its regulators RNF125 and TRIM25 in HIV-1-infected adult and pediatric individuals.

    PubMed

    Britto, Alan M A; Amoedo, Nívea D; Pezzuto, Paula; Afonso, Adriana O; Martínez, Ana M B; Silveira, Jussara; Sion, Fernando S; Machado, Elizabeth S; Soares, Marcelo A; Giannini, Ana L M

    2013-07-31

    TLRs (Toll-like receptors) and RLRs (RIG-I-like receptors) mediate innate immune responses by detecting microorganism invasion. RIG-I activation results in the production of interferon (IFN) type 1 and IFN responsive genes (ISGs). As the ubiquitin ligases RNF125 and TRIM25 are involved in regulating RIG-I function, our aim was to assess whether the levels of these three genes vary between healthy and HIV-infected individuals and whether these levels are related to disease progression. Gene expression analyses for RIG-I, RNF125, and TRIM25 were performed for HIV-infected adults and the children's peripheral blood mononuclear cells (PBMCs). Reverse transcription-quantitative PCRs (RT-qPCRs) were performed in order to quantify the expression levels of RIG-I, RNF125 and TRIM25 from PBMCs purified from control or HIV-infected individuals. Controls express higher levels of the three genes when compared to HIV-infected patients. These expressions are clearly distinct between healthy and progressors, and are reproduced in adults and children. In controls, RNF125 is the highest expressed gene, whereas in progressors, RIG-I is either the highest expressed gene or is expressed similarly to RNF125 and TRIM25. A pattern of expression of RIG-I, RNF125, and TRIM25 genes in HIV patients is evident. The high expression of RNF125 in healthy individuals reflects the importance of keeping RIG-I function off, inhibiting unnecessary IFN production. Consistent with this assumption, RNF125 levels are lower in HIV patients and importantly, the RNF125/RIG-I ratio is lower in patients who progress to AIDS. Our results might help to predict disease progression and unveil the role of poorly characterized host genes during HIV infection.

  17. Overpressure Prediction From Seismic Data: Implications on Drilling Safety

    NASA Astrophysics Data System (ADS)

    Osinowo, O. O.; Oladunjoye, M. A.; Olayinka, A. I.

    2007-12-01

    High rate of sediment influx into the Niger Delta via river Niger coupled with high rate of basin subsidence, very thick clayey members of Agbada and Akata Formations as well as prevailing presence of growth faults had been identified as the main factors responsible for overpressure generation and preservation in the Niger Delta basin. Analysis of porosity dependent parameters such as interval transit times and interval velocities derived from the seismic records of a field in the Western Niger Delta revealed the presence of overpressured formation at depth of 8670 feet, which is the top of the overpressured zone. The plot of interval transit times against depth gave a positive deflection from normal at the region of overpressure while interval velocity plot gave negative deflection; the ratio of this deviation in both cases is as high as 1.52. Pressure gradient in the upper, normally pressured part of the field was determined to be 0.465 psi/ft., which is within the established normal pressure gradient range in Niger Delta, while the abnormal formation pressure gradient in the overpressured region was determined to be 0.96 psi/ft., and this is also within the published abnormal pressure gradient range of 0.71 to 1.1 psi/ft. in Niger Delta. Formation fracture pressure gradients were determined from the formation pressure information to be 0.66psi/ft. in the upper part of the field and 1.2psi/ft. in the overpressured horizon. Mud weight window (MWW); mud density range necessary to prevent formation kick without initiating hydraulic fracturing was determined to be 10.2 to 12.5lbm/gal in the upper part of the field and 22.1 to 22.63lbm/gal in the overpressured horizon. MWW is indispensable for the selection of the mud pump type, capacity, pumping rate and mud densities at different formation pressure regimes. Overpressure prediction is also requisite for drilling program design, casing design as well as rig capacity choice before spudding. It is necessary to reduce well construction risk, save drilling hour as well as cut down drilling cost. If adequate predictions are not taken however, drilling hazards known as blowout may occur. Blowout, an uncontrollable flow of formation fluid into the well has made oil exploration and exploitation activities in Niger Delta, Southern Nigeria, a curse for the people rather than a blessing because considerable numbers of wells blew out during well construction activities, hence the characteristic oil spill which had degraded the environment, making fishing operation, a source of livelihood of the people difficult. Therefore the need for overpressure prediction as a guide for safe drilling, especially in unfamiliar exploration environments.

  18. In-situ test site at the International Geothermal Centre Bochum

    NASA Astrophysics Data System (ADS)

    Bracke, Rolf; Wittig, Volker; Güldenhaupt, Jonas; Duda, Mandy; Stöckhert, Ferdinand; Bussmann, Gregor; Tünte, Henry; Saenger, Erik H.; Eicker, Timm; Löer, Katrin; Schäfers, Klaus; Macit, Osman; Jagert, Felix

    2017-04-01

    The in-situ test site at the International Geothermal Centre (GZB) is located on the campus of the Bochum University of Applied Sciences. The area represents a 10.000 m2 drill site with existing research, observation and production wells and allows further drill tests and drilling down to depths of 5.000 m - also in conjunction with the approved authorized 50 km2 mining area "Future Energies" and the GZB's own mobile dual drive drilling rig Bo.Rex capable of drilling down to depths of 1000 m. The site allows for a comprehensive characterization of the subsurface underneath the university's campus in terms of a case study in Bochum pursuing the objective to provide an in-situ test field to researchers from geosciences and other disciplines. The local geology comprises folded and fractured carboniferous sediments including sandstones, siltstones, claystones and coal seams with low matrix permeabilies. Currently, one research well, 29 production wells, and seven monitoring wells are available. The research well reaching to a depth of about 500 m with an open-hole section between 450 m and 500 m has been fully cored down to 200 m, selected sections were additionally cored down to 450 m. Production wells with depths of up to 200 m are equipped with borehole heat exchangers providing heating and cooling for the GZB and a new lecture building. Monitoring wells vary in depth and reach down to 200 m. The majority of wells were comprehensively characterized using the GZB's borehole geophysical logging system with deviation, caliper, gamma ray and acoustic imaging, but also full waveform sonic, flowmeter and electrical conductivity. Cuttings were collected, documented and partly stored. The in-situ test site will be complemented by a seismic and hydrogeological observatory comprising borehole seismometers at depths of up to 200 m. The seismic network will ensure permanent observation of natural and potential anthropogenic seismicity. Additionally, drilling activities interpreted as seismic source can be used to develop a better understanding of the geological and geophysical structure of the subsurface. Hydrogeological monitoring wells will be used for field experiments such as flowmeter tests, pumping tests or chemical analysis of groundwater. Synergies arise from linking the field-scale infrastructure with laboratory equipment at the GZB covering basic and advanced physicochemical characterization as well as high resolution 3D imaging technologies under high pressure and high temperature reservoir conditions at various scales from mm to m. The GZB invites students, researchers and interested parties to participate in and shape the GZB's in-situ research infrastructure activities by addressing fundamental and applied questions related to geothermal energy provision and georesources in general.

  19. RIG-I in RNA virus recognition

    PubMed Central

    Kell, Alison M.; Gale, Michael

    2015-01-01

    Antiviral immunity is initiated upon host recognition of viral products via non-self molecular patterns known as pathogen-associated molecular patterns (PAMPs). Such recognition initiates signaling cascades that induce intracellular innate immune defenses and an inflammatory response that facilitates development of the acquired immune response. The retinoic acid-inducible gene I (RIG-I) and the RIG-I-like receptor (RLR) protein family are key cytoplasmic pathogen recognition receptors that are implicated in the recognition of viruses across genera and virus families, including functioning as major sensors of RNA viruses, and promoting recognition of some DNA viruses. RIG-I, the charter member of the RLR family, is activated upon binding to PAMP RNA. Activated RIG-I signals by interacting with the adapter protein MAVS leading to a signaling cascade that activates the transcription factors IRF3 and NF-κB. These actions induce the expression of antiviral gene products and the production of type I and III interferons that lead to an antiviral state in the infected cell and surrounding tissue. RIG-I signaling is essential for the control of infection by many RNA viruses. Recently, RIG-I crosstalk with other pathogen recognition receptors and components of the inflammasome has been described. In this review, we discuss the current knowledge regarding the role of RIG-I in recognition of a variety of virus families and its role in programming the adaptive immune response through cross-talk with parallel arms of the innate immune system, including how RIG-I can be leveraged for antiviral therapy. PMID:25749629

  20. Freeze shoe sampler for the collection of hyporheic zone sediments and porewater.

    PubMed

    Bianchin, M; Smith, L; Beckie, R

    2015-01-01

    The Starr and Ingleton (1992) drive point piston sampler (DPPS) design was modified by fitting it with a Murphy and Herkelrath (1996) type sample-freezing drive shoe (SFDS), which uses liquid carbon dioxide as a cryogen. Liquid carbon dioxide was used to freeze sediments in the lower 0.1 m of the core and the drive-point piston sealed the core at the top preserving the reductive-oxidation (redox) sensitive sediments from the atmosphere and maintaining natural stratigraphy. The use of nitrogen gas to provide positive pressure on the gas system blocked the ingress of water which froze on contact with the cryogen thus blocking the gas lines with ice. With this adaptation to the gas system cores could be collected at greater depths beneath the static water level. This tool was used to collect intact saturated sediment cores from the hyporheic zone of the tidally influenced Fraser River in Vancouver, British Columbia, Canada where steep geochemical and microbial gradients develop within the interface between discharging anaerobic groundwater and recharging aerobic river water. In total, 25 cores driven through a 1.5 m sampling interval were collected from the river bed with a mean core recovery of 75%. The ability to deploy this method from a fishing vessel makes the tool more cost effective than traditional marine-based drilling operations which often use barges, tug boats, and drilling rigs. © 2014, National Ground Water Association.

  1. Novel approach for the refractive index gradient measurement in microliter volumes using fiber-optic technology

    NASA Astrophysics Data System (ADS)

    Synovec, Robert E.; Renn, Curtiss N.

    1991-07-01

    The refractive index gradient (RIG) of hydrodynamically controlled profiles can be universally, yet sensitively, measured by carefully probing the radial RIG passing through a z-configuration flow cell. Fiber optic technology is applied in order to provide a narrow, collimated probe beam (100 micrometers diameter) that is deflected by a RIG and measured by a position sensitive detector. The fiber optic construction allows one to probe very small volumes (1 (mu) L to 3 (mu) L) amenable to microbore liquid chromatography ((mu) LC). The combination of (mu) LC and RIG detection is very useful for the analysis of trace quantities (ng injected amounts) of chemical species that are generally difficult to measure, i.e., species that are not amenable to absorbance detection or related techniques. Furthermore, the RIG detector is compatible with conventional mobile phase gradient and thermal gradient (mu) LC, unlike traditional RI detectors. A description of the RIG detector coupled with (mu) LC for the analysis of complex polymer samples is reported. Also, exploration into using the RIG detector for supercritical fluid chromatography is addressed.

  2. [Innate immune response to RNA virus infection].

    PubMed

    Oshiumi, Hiroyuki; Matsumoto, Misako; Seya, Tsukasa

    2011-12-01

    Viral RNA is recognized by RIG-I-like receptors and Toll-like receptors. RIG-I is a cytoplasmic viral RNA sensor. High Mobility Group Box (HMGB) proteins and DExD/H box RNA helicases, such as DDX3 and 60, associate with viral RNA. Those proteins promotes the RIG-I binding to viral RNA. RIG-I triggers the signal via IPS-1 adaptor molecule to induce type I IFN. RIG-I harbors Lys63-linked polyubiquitination by Riplet and TRIM25 ubiquitin ligases. The polyubiquitination is essential for RIG-I-mediated signaling. Toll-like receptors are located in endosome. TLR3 recognizes viral double-stranded RNA, and TLR7 and 8 recognize single-strand RNA. Virus has the ability to suppress these innate immune response. For example, to inhibit RIG-I-mediated signaling, HCV core protein suppresses the function of DDX3. In addition, HCV NS3-4A protein cleaves IPS-1 to inhibit the signal. Molecular mechanism of how viral RNA is recognized by innate immune system will make great progress on our understanding of how virus escapes from host immune system.

  3. Structural and functional insights into 5'-ppp RNA pattern recognition by the innate immune receptor RIG-I.

    PubMed

    Wang, Yanli; Ludwig, Janos; Schuberth, Christine; Goldeck, Marion; Schlee, Martin; Li, Haitao; Juranek, Stefan; Sheng, Gang; Micura, Ronald; Tuschl, Thomas; Hartmann, Gunther; Patel, Dinshaw J

    2010-07-01

    RIG-I is a cytosolic helicase that senses 5'-ppp RNA contained in negative-strand RNA viruses and triggers innate antiviral immune responses. Calorimetric binding studies established that the RIG-I C-terminal regulatory domain (CTD) binds to blunt-end double-stranded 5'-ppp RNA a factor of 17 more tightly than to its single-stranded counterpart. Here we report on the crystal structure of RIG-I CTD bound to both blunt ends of a self-complementary 5'-ppp dsRNA 12-mer, with interactions involving 5'-pp clearly visible in the complex. The structure, supported by mutation studies, defines how a lysine-rich basic cleft within the RIG-I CTD sequesters the observable 5'-pp of the bound RNA, with a stacked phenylalanine capping the terminal base pair. Key intermolecular interactions observed in the crystalline state are retained in the complex of 5'-ppp dsRNA 24-mer and full-length RIG-I under in vivo conditions, as evaluated from the impact of binding pocket RIG-I mutations and 2'-OCH(3) RNA modifications on the interferon response.

  4. Mitochondrially localised MUL1 is a novel modulator of antiviral signaling.

    PubMed

    Jenkins, Kristie; Khoo, Jing Jing; Sadler, Anthony; Piganis, Rebecca; Wang, Die; Borg, Natalie A; Hjerrild, Kathryn; Gould, Jodee; Thomas, Belinda J; Nagley, Phillip; Hertzog, Paul J; Mansell, Ashley

    2013-04-01

    The innate immune response to virus must be balanced to eliminate infection yet limit damaging inflammation. A critical arm of the antiviral response is launched by the retinoic acid-inducible-gene I (RIG-I) protein. RIG-I is activated by viral RNA then associates with the mitochondrial antiviral signaling (MAVS) protein to subsequently induce potent inflammatory cytokines. Here, we demonstrate the mitochondrial E3 ubiquitin protein ligase 1 (MUL1) is a crucial moderator of RIG-I signaling. MUL1 is localized to the mitochondria where it interacts with MAVS and catalyzes RIG-I post-translational modifications that inhibit RIG-I-dependent cell signaling. Accordingly, depletion of MUL1 potentiated RIG-I mediated nuclear factor-kappa B (NF-κB) and interferon (IFN) β reporter activity. Moreover, depletion of MUL1 boosted the antiviral response and increased proinflammatory cytokines following challenge with the RNA mimetic poly I:C and Sendai virus. We therefore submit that MUL1 is a novel regulator of the RIG-I-like receptor-dependent antiviral response, that otherwise functions to limit inflammation.

  5. Roof Weakening of Hydraulic Fracturing for Control of Hanging Roof in the Face End of High Gassy Coal Longwall Mining: A Case Study

    NASA Astrophysics Data System (ADS)

    Huang, Bingxiang; Wang, Youzhuang

    2016-09-01

    The occurence of hanging roof commonly arises in the face end of longwall coal mining under hard roof conditions. The sudden break and subsequent caving of a hanging roof could result in the extrusion of gas in the gob to the face, causing gas concentrations to rise sharply and to increase to over a safety-limited value. A series of linear fracturing-holes of 32 mm diameter were drilled into the roof of the entries with an anchor rig. According to the theory that the gob should be fully filled with the fragmentized falling roof rock, the drilling depth is determined as being 3 5 times the mining height if the broken expansion coefficient takes an empirical value. Considering the general extension range of cracks and the supporting form of the entryway, the spacing distance between two drilling holes is determined as being 1 2 times the crack's range of extension. Using a mounting pipe, a high pressure resistant sealing device of a small diameter-size was sent to the designated location for the high-pressure hydraulic fracturing of the roof rock. The hydraulic fracturing created the main hydro-fracturing crack and airfoil branch cracks in the interior of the roof-rock, transforming the roof structure and weakening the strength of the roof to form a weak plane which accelerated roof caving, and eventually induced the full caving in of the roof in time with the help of ground pressure. For holes deeper than 4 m, retreating hydraulic fracturing could ensure the uniformity of crack extension. Tested and applied at several mines in Shengdong Mining District, the highest ruptured water pressure was found to be 55 MPa, and the hanging roof at the face end was reduced in length from 12 m to less than 1 2 m. This technology has eliminated the risk of the extrusion of gas which has accumulated in the gob.

  6. Improved Main Shaft Seal Life in Gas Turbines Using Laser Surface Texturing

    NASA Astrophysics Data System (ADS)

    McNickle, Alan D.; Etsion, Izhak

    2002-10-01

    This paper presents a general overview of the improved main shaft seal life in gas turbines using laser surface texturing (LST). The contents include: 1) Laser Surface Texturing System; 2) Seal Schematic with LST applied; 3) Dynamic Rig Tests; 4) Surface Finish Definitions; 5) Wear Test Rig; 6) Dynamic Test Rig; 7) Seal Cross Section-Rig Test; and 8) Typical Test Results. This paper is in viewgraph form.

  7. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bradonjic, Milan; Hagberg, Aric; Hengartner, Nick

    We analyze component evolution in general random intersection graphs (RIGs) and give conditions on existence and uniqueness of the giant component. Our techniques generalize the existing methods for analysis on component evolution in RIGs. That is, we analyze survival and extinction properties of a dependent, inhomogeneous Galton-Watson branching process on general RIGs. Our analysis relies on bounding the branching processes and inherits the fundamental concepts from the study on component evolution in Erdos-Renyi graphs. The main challenge becomes from the underlying structure of RIGs, when the number of offsprings follows a binomial distribution with a different number of nodes andmore » different rate at each step during the evolution. RIGs can be interpreted as a model for large randomly formed non-metric data sets. Besides the mathematical analysis on component evolution, which we provide in this work, we perceive RIGs as an important random structure which has already found applications in social networks, epidemic networks, blog readership, or wireless sensor networks.« less

  8. Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Devarkar, Swapnil C.; Wang, Chen; Miller, Matthew T.

    The cytosolic innate immune receptor Retinoic Acid Inducible Gene-I (RIG-I) is the principal detector of pathogenic RNAs carrying a 5'-triphosphate (5'ppp). Self RNAs like mRNAs evade recognition by RIG-I due to posttranscriptional modifications like 5'-end capping with 7-methyl guanosine (m7G) and 2'-O-methylation of 5'-end nucleotides. Viruses have also evolved mechanisms to mimic these modifications, which in part is believed to aid in immune evasion. Currently, it is unclear how these modifications modulate RIG-I recognition. This paper provides structural and mechanistic insights into the roles of the m7G cap and 2'-O-methylation in RIG-I evasion. We show that RIG-I accommodates the m7Gmore » base while maintaining the 5'ppp contacts and can recognize Cap-0 RNAs but not Cap-1.« less

  9. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Halvorsen, T.

    The next generation subsea developments will be facing a number of new challenges which have to be solved to maintain a cost-efficient solution for production of oil and gas: (1) Smaller fields, i.e. cost reduction through volume will no longer be valid. (2) Freedom in configuration of subsea development. The current idea of standardization will not be directly applicable for cost reduction. (3) Various water depth. The same technology should be applicable for both guideline- and guideline less water depth. (4) Development in new areas of the world where drilling rig deployable system is a must. (5) Various types ofmore » fluid processing may be required as an integral part of a subsea production system. The next generation subsea production system should be universal and applicable to any subsea field development. Kongsberg Offshore a.s. (KOS) have gained extensive experience in supplying standardized total subsea systems. The paper presents the approach taken by KOS to develop the next generation subsea system, and discussed the challenges associated with this.« less

  10. Layout Design of Human-Machine Interaction Interface of Cabin Based on Cognitive Ergonomics and GA-ACA.

    PubMed

    Deng, Li; Wang, Guohua; Yu, Suihuai

    2016-01-01

    In order to consider the psychological cognitive characteristics affecting operating comfort and realize the automatic layout design, cognitive ergonomics and GA-ACA (genetic algorithm and ant colony algorithm) were introduced into the layout design of human-machine interaction interface. First, from the perspective of cognitive psychology, according to the information processing process, the cognitive model of human-machine interaction interface was established. Then, the human cognitive characteristics were analyzed, and the layout principles of human-machine interaction interface were summarized as the constraints in layout design. Again, the expression form of fitness function, pheromone, and heuristic information for the layout optimization of cabin was studied. The layout design model of human-machine interaction interface was established based on GA-ACA. At last, a layout design system was developed based on this model. For validation, the human-machine interaction interface layout design of drilling rig control room was taken as an example, and the optimization result showed the feasibility and effectiveness of the proposed method.

  11. Layout Design of Human-Machine Interaction Interface of Cabin Based on Cognitive Ergonomics and GA-ACA

    PubMed Central

    Deng, Li; Wang, Guohua; Yu, Suihuai

    2016-01-01

    In order to consider the psychological cognitive characteristics affecting operating comfort and realize the automatic layout design, cognitive ergonomics and GA-ACA (genetic algorithm and ant colony algorithm) were introduced into the layout design of human-machine interaction interface. First, from the perspective of cognitive psychology, according to the information processing process, the cognitive model of human-machine interaction interface was established. Then, the human cognitive characteristics were analyzed, and the layout principles of human-machine interaction interface were summarized as the constraints in layout design. Again, the expression form of fitness function, pheromone, and heuristic information for the layout optimization of cabin was studied. The layout design model of human-machine interaction interface was established based on GA-ACA. At last, a layout design system was developed based on this model. For validation, the human-machine interaction interface layout design of drilling rig control room was taken as an example, and the optimization result showed the feasibility and effectiveness of the proposed method. PMID:26884745

  12. Case history of Yakin field: its development and sand control

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sawolo, N.; Krueger, R.F.; Maly, G.P.

    1983-01-01

    This paper deals with the development of the Yakin field in East Kalimantan, Indonesia, with emphasis on the sand control methods used. Implementation of an effective sand control program ensured the successful development of this field. Gravel-packed wells had substantially lower production decline rates than the initial completions without gravel packs. Control of sand production also has been demonstrated by the lack of sand problems during the 4 1/2 years since the sand control program was initiated. During this time there have been no submersible pump failures associated with sand production. The successful sand control program was achieved by amore » well-coordinated and cooperative effort of drilling, reservoir engineering, production research, and service company personnel. Establishment of communication among all people involved, starting early in the planning process and continuing through the rig operations to the final production phase, coupled with intensive training at all levels of responsibility, on-site supervision, and quality control were important factors in the success of the development program.« less

  13. English-Spanish glossary: offshore exploration and production, gas processing, and valves (in Spanish)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1981-12-01

    This series of articles contains 3 different English-Spanish glossaries of related terms used in the oil industry. The glossary of the offshore exploration and production involves a summary of terms used in the offshore oil activity. It also includes names of singular equipment used in offshore drilling, as well as several navigation terms in relation to the floating oil structures. With the help of the Gas Processors Association it was possible to compile a glossary of gas processing with a concise selection of common terms of the industry of gas processing. The glossary of valves includes more than 200 termsmore » of the industry of valves in a specialized glossary, and several explanations about the application and operation of valves.« less

  14. Learning Inverse Rig Mappings by Nonlinear Regression.

    PubMed

    Holden, Daniel; Saito, Jun; Komura, Taku

    2017-03-01

    We present a framework to design inverse rig-functions-functions that map low level representations of a character's pose such as joint positions or surface geometry to the representation used by animators called the animation rig. Animators design scenes using an animation rig, a framework widely adopted in animation production which allows animators to design character poses and geometry via intuitive parameters and interfaces. Yet most state-of-the-art computer animation techniques control characters through raw, low level representations such as joint angles, joint positions, or vertex coordinates. This difference often stops the adoption of state-of-the-art techniques in animation production. Our framework solves this issue by learning a mapping between the low level representations of the pose and the animation rig. We use nonlinear regression techniques, learning from example animation sequences designed by the animators. When new motions are provided in the skeleton space, the learned mapping is used to estimate the rig controls that reproduce such a motion. We introduce two nonlinear functions for producing such a mapping: Gaussian process regression and feedforward neural networks. The appropriate solution depends on the nature of the rig and the amount of data available for training. We show our framework applied to various examples including articulated biped characters, quadruped characters, facial animation rigs, and deformable characters. With our system, animators have the freedom to apply any motion synthesis algorithm to arbitrary rigging and animation pipelines for immediate editing. This greatly improves the productivity of 3D animation, while retaining the flexibility and creativity of artistic input.

  15. Meals on Wheels? A Decade of Megafaunal Visual and Acoustic Observations from Offshore Oil & Gas Rigs and Platforms in the North and Irish Seas.

    PubMed

    Todd, Victoria Louise Georgia; Warley, Jane Clare; Todd, Ian Boyer

    2016-01-01

    A decade of visual and acoustic detections of marine megafauna around offshore Oil & Gas (O&G) installations in the North and Irish Seas are presented. Marine megafauna activity was monitored visually and acoustically by Joint Nature Conservation Committee (JNCC) qualified and experienced Marine Mammal Observers (MMO) and Passive Acoustic Monitoring (PAM) Operators respectively, with real-time towed PAM in combination with industry standard software, PAMGuard. Monitoring was performed during routine O&G industrial operations for underwater noise mitigation purposes, and to ensure adherence to regulatory guidelines. Incidental sightings by off-effort MMOs and installation crew were also reported. Visual and acoustic monitoring spanned 55 non-consecutive days between 2004 and 2014. A total of 47 marine mammal sightings were recorded by MMOs on dedicated watch, and 10 incidental sightings of marine megafauna were reported over 10 years. Species included: harbour porpoise (Phocoena phocoena), Atlantic white-sided dolphin (Lagenorhynchus acutus), white beaked dolphin (Lagenorhynchus albirostris), common dolphin (Delphinus delphis), minke whale (Balaenoptera acutorostrata), common seal (Phoca vitulina), grey seal (Halichoerus grypus) and, basking shark (Cetorhinus maximus). Passive Acoustic Monitoring was conducted on two occasions in 2014; 160 PAM hours over 12 days recorded a total of 308 individual clicks identified as harbour porpoises. These appear to be the first such acoustic detections obtained from a North Sea drilling rig whilst using a typically configured hydrophone array designed for towing in combination with real-time PAMGuard software. This study provides evidence that marine megafauna are present around mobile and stationary offshore O&G installations during routine operational activities. On this basis, Environmental Impact Assessments (EIAs) for decommissioning O&G platforms should be carried-out on a case-by-case basis, and must include provisions for hitherto overlooked marine megafauna.

  16. Robust Lys63-Linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection.

    PubMed

    Jiang, Jingwen; Li, Jing; Fan, Wenhui; Zheng, Weinan; Yu, Meng; Chen, Can; Sun, Lei; Bi, Yuhai; Ding, Chan; Gao, George F; Liu, Wenjun

    2016-07-15

    Influenza A and B virus infections both cause a host innate immunity response. Here, we report that the robust production of type I and III interferons (IFNs), IFN-stimulated genes, and proinflammatory factors can be induced by influenza B virus rather than influenza A virus infection in alveolar epithelial (A549) cells during early infection. This response is mainly dependent on the retinoic acid-inducible gene I (RIG-I)-mediated signaling pathway. Infection by influenza B virus promotes intense Lys63-linked ubiquitination of RIG-I, resulting in cytokine eruption. It is known that the influenza A virus NS1 protein (NS1-A) interacts with RIG-I and TRIM25 to suppress the activation of RIG-I-mediated signaling. However, the present results indicate that the influenza B virus NS1 protein (NS1-B) is unable to interact with RIG-I but engages in the formation of a RIG-I/TRIM25/NS1-B ternary complex. Furthermore, we demonstrate that the N-terminal RNA-binding domain (RBD) of NS1-B is responsible for interaction with TRIM25 and that this interaction blocks the inhibitory effect of the NS1-B C-terminal effector domain (TED) on RIG-I ubiquitination. Our findings reveal a novel mechanism for the host cytokine response to influenza B virus infection through regulatory interplay between host and viral proteins. Influenza B virus generally causes local mild epidemics but is occasionally lethal to individuals. Existing studies describe the broad characteristics of influenza B virus epidemiology and pathology. However, to develop better prevention and treatments for the disease, determining the concrete molecular mechanisms of pathogenesis becomes pivotal to understand how the host reacts to the challenge of influenza B virus. Thus, we aimed to characterize the host innate immune response to influenza B virus infection. Here, we show that vigorous Lys63-linked ubiquitination of RIG-I and cytokine eruption dependent on RIG-I-mediated signal transduction are induced by virus infection. Additionally, TRIM25 positively regulates RIG-I-mediated signaling by ablating the inhibitory function of NS1-B on RIG-I ubiquitination. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  17. Medical evacuations from oil rigs off the Gulf Coast of the United States from 2008 to 2012: reasons and cost implications.

    PubMed

    Thibodaux, Donald P; Bourgeois, Robert M; Loeppke, Ronald R; Konicki, Doris L; Hymel, Pamela A; Dreger, Marianne

    2014-07-01

    To identify reasons for air medical evacuations from oil rigs/platforms. Retrospective review of data of medical calls from 102 rigs/platforms in the US Gulf Coast from 2008 through 2012 with specific analysis of medevacs. On average, 1609 total calls per year relating to illness or injury on the 102 oil rigs/platforms with 4% to 7% requiring medical air evacuation. On average, 77% of medevacs were for nonoccupational medical injury or illness. Illness, not occupational injuries, is identified as the major reason for medical evacuations from oil rigs. Heart disease is the leading cause of chronic health conditions resulting in a medevac.

  18. Toscana Virus NSs Protein Inhibits the Induction of Type I Interferon by Interacting with RIG-I

    PubMed Central

    Gori-Savellini, Gianni; Valentini, Melissa

    2013-01-01

    Toscana virus (TOSV) is a phlebovirus, of the Bunyaviridae family, that is responsible for central nervous system (CNS) injury in humans. Previous data have shown that the TOSV NSs protein is a gamma interferon (IFN-β) antagonist when transiently overexpressed in mammalian cells, inhibiting IRF-3 induction (G. Gori Savellini, F. Weber, C. Terrosi, M. Habjan, B. Martorelli, and M. G. Cusi, J. Gen. Virol. 92:71–79, 2011). In this study, we investigated whether an upstream sensor, which has a role in the signaling cascade leading to the production of type I IFN, was involved. We found a significant decrease in RIG-I protein levels in cells overexpressing TOSV NSs, suggesting that the nonstructural protein interacts with RIG-I and targets it for proteasomal degradation. In fact, the MG-132 proteasome inhibitor was able to restore IFN-β promoter activation in cells expressing NSs, demonstrating the existence of an evasion mechanism based on inhibition of the RIG-I sensor. Furthermore, a C-terminal truncated NSs protein (ΔNSs), although able to interact with RIG-I, did not affect the RIG-I-mediated IFN-β promoter activation, suggesting that the NSs domains responsible for RIG-I-mediated signaling and interaction with RIG-I are mapped on different regions. These results contribute to identify a novel mechanism for bunyaviruses by which TOSV NSs counteracts the early IFN response. PMID:23552410

  19. Toscana virus NSs protein inhibits the induction of type I interferon by interacting with RIG-I.

    PubMed

    Gori-Savellini, Gianni; Valentini, Melissa; Cusi, Maria Grazia

    2013-06-01

    Toscana virus (TOSV) is a phlebovirus, of the Bunyaviridae family, that is responsible for central nervous system (CNS) injury in humans. Previous data have shown that the TOSV NSs protein is a gamma interferon (IFN-β) antagonist when transiently overexpressed in mammalian cells, inhibiting IRF-3 induction (G. Gori Savellini, F. Weber, C. Terrosi, M. Habjan, B. Martorelli, and M. G. Cusi, J. Gen. Virol. 92:71-79, 2011). In this study, we investigated whether an upstream sensor, which has a role in the signaling cascade leading to the production of type I IFN, was involved. We found a significant decrease in RIG-I protein levels in cells overexpressing TOSV NSs, suggesting that the nonstructural protein interacts with RIG-I and targets it for proteasomal degradation. In fact, the MG-132 proteasome inhibitor was able to restore IFN-β promoter activation in cells expressing NSs, demonstrating the existence of an evasion mechanism based on inhibition of the RIG-I sensor. Furthermore, a C-terminal truncated NSs protein (ΔNSs), although able to interact with RIG-I, did not affect the RIG-I-mediated IFN-β promoter activation, suggesting that the NSs domains responsible for RIG-I-mediated signaling and interaction with RIG-I are mapped on different regions. These results contribute to identify a novel mechanism for bunyaviruses by which TOSV NSs counteracts the early IFN response.

  20. rigor mortis encodes a novel nuclear receptor interacting protein required for ecdysone signaling during Drosophila larval development.

    PubMed

    Gates, Julie; Lam, Geanette; Ortiz, José A; Losson, Régine; Thummel, Carl S

    2004-01-01

    Pulses of the steroid hormone ecdysone trigger the major developmental transitions in Drosophila, including molting and puparium formation. The ecdysone signal is transduced by the EcR/USP nuclear receptor heterodimer that binds to specific response elements in the genome and directly regulates target gene transcription. We describe a novel nuclear receptor interacting protein encoded by rigor mortis (rig) that is required for ecdysone responses during larval development. rig mutants display defects in molting, delayed larval development, larval lethality, duplicated mouth parts, and defects in puparium formation--phenotypes that resemble those seen in EcR, usp, E75A and betaFTZ-F1 mutants. Although the expression of these nuclear receptor genes is essentially normal in rig mutant larvae, the ecdysone-triggered switch in E74 isoform expression is defective. rig encodes a protein with multiple WD-40 repeats and an LXXLL motif, sequences that act as specific protein-protein interaction domains. Consistent with the presence of these elements and the lethal phenotypes of rig mutants, Rig protein interacts with several Drosophila nuclear receptors in GST pull-down experiments, including EcR, USP, DHR3, SVP and betaFTZ-F1. The ligand binding domain of betaFTZ-F1 is sufficient for this interaction, which can occur in an AF-2-independent manner. Antibody stains reveal that Rig protein is present in the brain and imaginal discs of second and third instar larvae, where it is restricted to the cytoplasm. In larval salivary gland and midgut cells, however, Rig shuttles between the cytoplasm and nucleus in a spatially and temporally regulated manner, at times that correlate with the major lethal phase of rig mutants and major switches in ecdysone-regulated gene expression. Taken together, these data indicate that rig exerts essential functions during larval development through gene-specific effects on ecdysone-regulated transcription, most likely as a cofactor for one or more nuclear receptors. Furthermore, the dynamic intracellular redistribution of Rig protein suggests that it may act to refine spatial and temporal responses to ecdysone during development.

  1. Structural basis of RNA recognition and activation by innate immune receptor RIG-I

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jiang, Fuguo; Ramanathan, Anand; Miller, Matthew T.

    Retinoic-acid-inducible gene-I (RIG-I; also known as DDX58) is a cytoplasmic pathogen recognition receptor that recognizes pathogen-associated molecular pattern (PAMP) motifs to differentiate between viral and cellular RNAs. RIG-I is activated by blunt-ended double-stranded (ds)RNA with or without a 5'-triphosphate (ppp), by single-stranded RNA marked by a 5'-ppp and by polyuridine sequences. Upon binding to such PAMP motifs, RIG-I initiates a signalling cascade that induces innate immune defences and inflammatory cytokines to establish an antiviral state. The RIG-I pathway is highly regulated and aberrant signalling leads to apoptosis, altered cell differentiation, inflammation, autoimmune diseases and cancer. The helicase and repressor domainsmore » (RD) of RIG-I recognize dsRNA and 5'-ppp RNA to activate the two amino-terminal caspase recruitment domains (CARDs) for signalling. Here, to understand the synergy between the helicase and the RD for RNA binding, and the contribution of ATP hydrolysis to RIG-I activation, we determined the structure of human RIG-I helicase-RD in complex with dsRNA and an ATP analogue. The helicase-RD organizes into a ring around dsRNA, capping one end, while contacting both strands using previously uncharacterized motifs to recognize dsRNA. Small-angle X-ray scattering, limited proteolysis and differential scanning fluorimetry indicate that RIG-I is in an extended and flexible conformation that compacts upon binding RNA. These results provide a detailed view of the role of helicase in dsRNA recognition, the synergy between the RD and the helicase for RNA binding and the organization of full-length RIG-I bound to dsRNA, and provide evidence of a conformational change upon RNA binding. The RIG-I helicase-RD structure is consistent with dsRNA translocation without unwinding and cooperative binding to RNA. The structure yields unprecedented insight into innate immunity and has a broader impact on other areas of biology, including RNA interference and DNA repair, which utilize homologous helicase domains within DICER and FANCM.« less

  2. Linear ubiquitin assembly complex negatively regulates RIG-I and TRIM25 mediated type-I interferon induction

    PubMed Central

    Inn, Kyung-Soo; Gack, Michaela U.; Tokunaga, Fuminori; Shi, Mude; Wong, Lai-Yee; Iwai, Kazuhiro; Jung, Jae U.

    2011-01-01

    Summary Upon detection of viral RNA, retinoic acid inducible gene I (RIG-I) undergoes TRIM25-mediated Lys-63 linked ubiquitination, leading to type-I interferon (IFN) production. In this study, we demonstrate that the linear ubiquitin assembly complex (LUBAC), comprised of two RING-IBR-RING (RBR)-containing E3 ligases HOIL-1L and HOIP, independently targets TRIM25 and RIG-I to effectively suppress virus-induced IFN production. RBR E3 ligase domains of HOIL-1L and HOIP bind and induce proteosomal degradation of TRIM25, whereas the NZF domain of HOIL-1L competes with TRIM25 for RIG-I binding. Consequently, both actions by the HOIL-1L/HOIP LUBAC potently inhibit RIG-I ubiquitination and anti-viral activity, but in a mechanistically separate manner. Conversely, the genetic deletion or depletion of HOIL-1L and HOIP robustly enhances virus-induced type-I IFN production. Taken together, the HOIL-1L/HOIP LUBAC specifically suppresses RIG-I ubiquitination and activation by inducing TRIM25 degradation and inhibiting TRIM25 interaction with RIG-I, resulting in the comprehensive suppression of the IFN-mediated anti-viral signaling pathway. PMID:21292167

  3. Linear ubiquitin assembly complex negatively regulates RIG-I- and TRIM25-mediated type I interferon induction.

    PubMed

    Inn, Kyung-Soo; Gack, Michaela U; Tokunaga, Fuminori; Shi, Mude; Wong, Lai-Yee; Iwai, Kazuhiro; Jung, Jae U

    2011-02-04

    Upon detection of viral RNA, retinoic acid-inducible gene I (RIG-I) undergoes TRIM25-mediated K63-linked ubiquitination, leading to type I interferon (IFN) production. In this study, we demonstrate that the linear ubiquitin assembly complex (LUBAC), comprised of two RING-IBR-RING (RBR)-containing E3 ligases, HOIL-1L and HOIP, independently targets TRIM25 and RIG-I to effectively suppress virus-induced IFN production. RBR E3 ligase domains of HOIL-1L and HOIP bind and induce proteasomal degradation of TRIM25, whereas the NZF domain of HOIL-1L competes with TRIM25 for RIG-I binding. Consequently, both actions by the HOIL-1L/HOIP LUBAC potently inhibit RIG-I ubiquitination and antiviral activity, but in a mechanistically separate manner. Conversely, the genetic deletion or depletion of HOIL-1L and HOIP robustly enhances virus-induced type I IFN production. Taken together, the HOIL-1L/HOIP LUBAC specifically suppresses RIG-I ubiquitination and activation by inducing TRIM25 degradation and inhibiting TRIM25 interaction with RIG-I, resulting in the comprehensive suppression of the IFN-mediated antiviral signaling pathway. Copyright © 2011 Elsevier Inc. All rights reserved.

  4. Insight into Buffalo (Bubalus bubalis) RIG1 and MDA5 Receptors: A Comparative Study on dsRNA Recognition and In-Vitro Antiviral Response

    PubMed Central

    Singh, Manvender; Brahma, Biswajit; Maharana, Jitendra; Patra, Mahesh Chandra; Kumar, Sushil; Mishra, Purusottam; Saini, Megha; De, Bidhan Chandra; Mahanty, Sourav; Datta, Tirtha Kumar; De, Sachinandan

    2014-01-01

    RIG1 and MDA5 have emerged as important intracellular innate pattern recognition receptors that recognize viral RNA and mediate cellular signals controlling Type I interferon (IFN-I) response. Buffalo RIG1 and MDA5 genes were investigated to understand the mechanism of receptor induced antiviral response. Sequence analysis revealed that RIG1 and MDA5 maintain a domain arrangement that is common in mammals. Critical binding site residues of the receptors are evolutionary conserved among mammals. Molecular dynamics simulations suggested that RIG1 and MDA5 follow a similar, if not identical, dsRNA binding pattern that has been previously reported in human. Moreover, binding free energy calculation revealed that MDA5 had a greater affinity towards dsRNA compared to RIG1. Constitutive expressions of RLR genes were ubiquitous in different tissues without being specific to immune organs. Poly I:C stimulation induced elevated expressions of IFN-β and IFN-stimulated genes (ISGs) through interferon regulatory factors (IRFs) mediated pathway in buffalo foetal fibroblast cells. The present study provides crucial insights into the structure and function of RIG1 and MDA5 receptors in buffalo. PMID:24587036

  5. Ceramic applications in turbine engines

    NASA Technical Reports Server (NTRS)

    Byrd, J. A.; Janovicz, M. A.; Thrasher, S. R.

    1981-01-01

    Development testing activities on the 1900 F-configuration ceramic parts were completed, 2070 F-configuration ceramic component rig and engine testing was initiated, and the conceptual design for the 2265 F-configuration engine was identified. Fabrication of the 2070 F-configuration ceramic parts continued, along with burner rig development testing of the 2070 F-configuration metal combustor in preparation for 1132 C (2070 F) qualification test conditions. Shakedown testing of the hot engine simulator (HES) rig was also completed in preparation for testing of a spin rig-qualified ceramic-bladed rotor assembly at 1132 C (2070 F) test conditions. Concurrently, ceramics from new sources and alternate materials continued to be evaluated, and fabrication of 2070 F-configuration ceramic component from these new sources continued. Cold spin testing of the critical 2070 F-configuration blade continued in the spin test rig to qualify a set of ceramic blades at 117% engine speed for the gasifier turbine rotor. Rig testing of the ceramic-bladed gasifier turbine rotor assembly at 108% engine speed was also performed, which resulted in the failure of one blade. The new three-piece hot seal with the nickel oxide/calcium fluoride wearface composition was qualified in the regenerator rig and introduced to engine operation wiwth marginal success.

  6. Rich burn combustor technology at Pratt and Whitney

    NASA Technical Reports Server (NTRS)

    Lohmann, Robert P.; Rosfjord, T. J.

    1992-01-01

    The topics covered include the following: near term objectives; rich burn quick quench combustor (RBQC); RBQC critical technology areas; cylindrical RBQQ combustor rig; modular RBQQ combustor; cylindrical rig objectives; quench zone mixing; noneffusive cooled liner; variable geometry requirements; and sector combustor rig.

  7. Temporal and spatial patterns of habitat use by juveniles of a small coastal shark (Mustelus lenticulatus) in an estuarine nursery.

    PubMed

    Francis, Malcolm P

    2013-01-01

    Juvenile rig (Mustelus lenticulatus) were internally tagged with acoustic transmitters and tracked with acoustic receivers deployed throughout two arms of Porirua Harbour, a small (7 km(2)) estuary in New Zealand. Ten rig were tracked for up to four months during summer-autumn to determine their spatial and temporal use of the habitat. The overall goal was to estimate the size of Marine Protected Areas required to protect rig nursery areas from direct human impacts. Rig showed clear site preferences, but those preferences varied among rig and over time. They spent most of their time in large basins and on shallow sand and mud flats around the margins, and avoided deep channels. Habitat range increased during autumn for many of the rig. Only one shark spent time in both harbour arms, indicating that there was little movement between the two. Rig home ranges were 2-7 km(2), suggesting that an effective MPA would need to cover the entire Porirua Harbour. They moved to outer harbour sites following some high river flow rates, and most left the harbour permanently during or soon after a river spike, suggesting that they were avoiding low salinity water. Rig showed strong diel movements during summer, although the diel pattern weakened in autumn. Persistent use of the same day and night sites indicates that diel movements are directed rather than random. Further research is required to determine the sizes of rig home ranges in larger harbours where nursery habitat is more extensive. Marine Protected Areas do not control land-based impacts such as accelerated sedimentation and heavy metal pollution, so integration of marine and terrestrial management tools across a range of government agencies is essential to fully protect nursery areas.

  8. Low Frequency Noise Contamination in Fan Model Testing

    NASA Technical Reports Server (NTRS)

    Brown, Clifford A.; Schifer, Nicholas A.

    2008-01-01

    Aircraft engine noise research and development depends on the ability to study and predict the noise created by each engine component in isolation. The presence of a downstream pylon for a model fan test, however, may result in noise contamination through pylon interactions with the free stream and model exhaust airflows. Additionally, there is the problem of separating the fan and jet noise components generated by the model fan. A methodology was therefore developed to improve the data quality for the 9 15 Low Speed Wind Tunnel (LSWT) at the NASA Glenn Research Center that identifies three noise sources: fan noise, jet noise, and rig noise. The jet noise and rig noise were then measured by mounting a scale model of the 9 15 LSWT model fan installation in a jet rig to simulate everything except the rotating machinery and in duct components of fan noise. The data showed that the spectra measured in the LSWT has a strong rig noise component at frequencies as high as 3 kHz depending on the fan and airflow fan exit velocity. The jet noise was determined to be significantly lower than the rig noise (i.e., noise generated by flow interaction with the downstream support pylon). A mathematical model for the rig noise was then developed using a multi-dimensional least squares fit to the rig noise data. This allows the rig noise to be subtracted or removed, depending on the amplitude of the rig noise relative to the fan noise, at any given frequency, observer angle, or nozzle pressure ratio. The impact of isolating the fan noise with this method on spectra, overall power level (OAPWL), and Effective Perceived Noise Level (EPNL) is studied.

  9. Temporal and Spatial Patterns of Habitat Use by Juveniles of a Small Coastal Shark (Mustelus lenticulatus) in an Estuarine Nursery

    PubMed Central

    Francis, Malcolm P.

    2013-01-01

    Juvenile rig (Mustelus lenticulatus) were internally tagged with acoustic transmitters and tracked with acoustic receivers deployed throughout two arms of Porirua Harbour, a small (7 km2) estuary in New Zealand. Ten rig were tracked for up to four months during summer–autumn to determine their spatial and temporal use of the habitat. The overall goal was to estimate the size of Marine Protected Areas required to protect rig nursery areas from direct human impacts. Rig showed clear site preferences, but those preferences varied among rig and over time. They spent most of their time in large basins and on shallow sand and mud flats around the margins, and avoided deep channels. Habitat range increased during autumn for many of the rig. Only one shark spent time in both harbour arms, indicating that there was little movement between the two. Rig home ranges were 2–7 km2, suggesting that an effective MPA would need to cover the entire Porirua Harbour. They moved to outer harbour sites following some high river flow rates, and most left the harbour permanently during or soon after a river spike, suggesting that they were avoiding low salinity water. Rig showed strong diel movements during summer, although the diel pattern weakened in autumn. Persistent use of the same day and night sites indicates that diel movements are directed rather than random. Further research is required to determine the sizes of rig home ranges in larger harbours where nursery habitat is more extensive. Marine Protected Areas do not control land-based impacts such as accelerated sedimentation and heavy metal pollution, so integration of marine and terrestrial management tools across a range of government agencies is essential to fully protect nursery areas. PMID:23437298

  10. Five-Axis, Three-Magnetic-Bearing Dynamic Spin Rig

    NASA Technical Reports Server (NTRS)

    Morrison, Carlos R.; Provenza, Andrew; Kurkov, Anatole; Mehmed, Oral; Johnson, Dexter; Montague, Gerald; Duffy, Kirsten; Jansen, Ralph

    2005-01-01

    The Five-Axis, Three-Magnetic-Bearing Dynamic Spin Rig is an apparatus for vibration testing of turbomachine blades in a vacuum at rotational speeds from 0 to 40,000 rpm. This rig includes (1) a vertically oriented shaft on which is mounted an assembly comprising a rotor holding the blades to be tested, (2) two actively controlled heteropolar radial magnetic bearings at opposite ends of the shaft, and (3) an actively controlled magnetic thrust bearing at the upper end of the shaft. This rig is a more capable successor to a prior apparatus, denoted the Dynamic Spin Rig (DSR), that included a vertically oriented shaft with a mechanical thrust bearing at the upper end and a single actively controlled heteropolar radial magnetic bearing at the lower end.

  11. Cheating the Children.

    ERIC Educational Resources Information Center

    Rist, Marilee C.

    1993-01-01

    Schools are the victims, as bid-rigging scandals in the dairy-products industry surface in 13 states. School boards, in close cooperation with superintendents and district business managers, have the responsibility to detect bid-rigging or collusion. Offers guides for good business management and warning signs of bid-rigging. (MLF)

  12. Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway

    PubMed Central

    Sanchez, Jacint G.; Chiang, Jessica J.; Sparrer, Konstantin M.J.; Alam, Steven L.; Chi, Michael; Roganowicz, Marcin D.; Sankaran, Banumathi; Gack, Michaela U.; Pornillos, Owen

    2016-01-01

    SUMMARY Antiviral response pathways induce interferon by higher-order assembly of signaling complexes called signalosomes. Assembly of the RIG-I signalosome is regulated by K63-linked polyubiquitin chains, which are synthesized by the E3 ubiquitin ligase, TRIM25. We have previously shown that the TRIM25 coiled-coil domain is a stable, antiparallel dimer that positions two catalytic RING domains on opposite ends of an elongated rod. We now show that the RING domain is a separate self-association motif that engages ubiquitin-conjugated E2 enzymes as a dimer. RING dimerization is required for catalysis, TRIM25-mediated RIG-I ubiquitination, interferon induction, and antiviral activity. We also provide evidence that RING dimerization and E3 ligase activity are promoted by binding of the TRIM25 SPRY domain to the RIG-I effector domain. These results indicate that TRIM25 actively participates in higher-order assembly of the RIG-I signalosome and helps to fine-tune the efficiency of the RIG-I-mediated antiviral response. PMID:27425606

  13. Multi-Axis Test Facility

    NASA Image and Video Library

    1959-11-01

    Multi-Axis Test Facility, Space Progress Report, November 1, 1959: The Multi Axis Space Test Inertia Facility [MASTIF], informally referred to as the Gimbal Rig, was installed inside the Altitude Wind Tunnel. The rig, which spun on three axis simultaneously, was used to train the Mercury astronauts on how to bring a spinning spacecraft under control and to determine the effects of rapid spinning on the astronaut's eyesight and psyche. Small gaseous nitrogen jets were operated by the pilot to gain control of the rig after it had been set in motion. Part 1 shows pilot Joe Algranti in the rig as it rotates over one, two, and three axis. It also has overall views of the test set-up with researchers and technicians on the test platform. Part 2 shows Algranti being secured in the rig prior to the test. The rig is set in motion and the pilot slowly brings it under control. The Mercury astronauts trained on the MASTIF in early spring of 1960.

  14. Fabrication And Evaluation Of Sic/Sic Tubes With Various Fiber Architectures

    NASA Technical Reports Server (NTRS)

    Yun, H. M.; DiCarlo, J. A.; Fox, D. S.

    2003-01-01

    SiC/SiC composites are excellent material candidates for high temperature applications where the performance requirements are high strength, high creep-rupture resistance, high environmental durability, and high thermal conductivity. In the past, the NASA UEET program has demonstrated fabrication of high-performance SiC/SiC flat panels reinforced by Sylramic-iBN SiC fibers. Currently NASA UEET is scaling up this SiC/SiC system by fabrication of more complex shaped components using the same fiber type. This paper reports the effects of various fiber architectures on the processing, mechanical, and durability behavior of small-diameter 0.5" ID SiC/SiC tubes, which are potential sub-elements for leading edges and cooling channels in turbine vanes and blades. Nine different fiber architectures were utilized for construction of seamless tube preforms, from simple 2D jelly-rolling to complex braiding, pin-weaving, filament-winding and 3D orthogonal weaving with approximately 5% fibers in the thru-thickness direction. Using the BN interphase and Sic matrix processing steps established for the flat panels, SiC/SiC tubes were fabricated with wall thicknesses of approximately 60 mils and total fiber fractions of approximately 35%. The "D" split ring tests for hoop tensile properties, micro-structural examinations for relationship between fiber architecture formation and matrix infiltration, and the low-pressure burner rig tests for the high temperature durability under thru-thickness thermal gradient were conducted. The better matrix infiltration and higher hoop strength were achieved using the tri-axial braided and the three-float pin woven SiC/SiC tubes. In general, it needs not only higher hoop direction fibers but also axial direction fibers for the higher hoop strength and the better infiltration, respectively. These results are analyzed to offer general guidelines for selecting fiber pre-form architectures and SiC/SiC processes that maximize tube hoop strength, thru-thickness thermal conductivity, and burner-rig durability under a high thermal gradient.

  15. 49 CFR 230.111 - Spring rigging.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

  16. 49 CFR 230.111 - Spring rigging.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

  17. 49 CFR 230.111 - Spring rigging.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

  18. 49 CFR 230.111 - Spring rigging.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Spring rigging. 230.111 Section 230.111... Tenders Trucks, Frames and Equalizing System § 230.111 Spring rigging. (a) Arrangement of springs and equalizers. Springs and equalizers shall be arranged to ensure the proper distribution of weight to the...

  19. Including impacts of particulate emissions on marine ecosystems in life cycle assessment: the case of offshore oil and gas production.

    PubMed

    Veltman, Karin; Huijbregts, Mark A J; Rye, Henrik; Hertwich, Edgar G

    2011-10-01

    Life cycle assessment is increasingly used to assess the environmental performance of fossil energy systems. Two of the dominant emissions of offshore oil and gas production to the marine environment are the discharge of produced water and drilling waste. Although environmental impacts of produced water are predominantly due to chemical stressors, a major concern regarding drilling waste discharge is the potential physical impact due to particles. At present, impact indicators for particulate emissions are not yet available in life cycle assessment. Here, we develop characterization factors for 2 distinct impacts of particulate emissions: an increased turbidity zone in the water column and physical burial of benthic communities. The characterization factor for turbidity is developed analogous to characterization factors for toxic impacts, and ranges from 1.4 PAF (potentially affected fraction) · m(3) /d/kg(p) (kilogram particulate) to 7.0 x 10³ [corrected] for drilling mud particles discharged from the rig. The characterization factor for burial describes the volume of sediment that is impacted by particle deposition on the seafloor and equals 2.0 × 10(-1) PAF · m(3) /d/kg(p) for cutting particles. This characterization factor is quantified on the basis of initial deposition layer characteristics, such as height and surface area, the initial benthic response, and the recovery rate. We assessed the relevance of including particulate emissions in an impact assessment of offshore oil and gas production. Accordingly, the total impact on the water column and on the sediment was quantified based on emission data of produced water and drilling waste for all oil and gas fields on the Norwegian continental shelf in 2008. Our results show that cutting particles contribute substantially to the total impact of offshore oil and gas production on marine sediments, with a relative contribution of 55% and 31% on the regional and global scale, respectively. In contrast, the contribution of particulate emissions to the total impact on the marine water column is of minor importance. We conclude that particles are an important stressor in marine ecosystems, particularly for marine sediment, and particulate emissions should therefore be included in a (life cycle) impact assessment of offshore oil and gas production. Copyright © 2011 SETAC.

  20. The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling.

    PubMed

    Chiang, Cindy; Pauli, Eva-Katharina; Biryukov, Jennifer; Feister, Katharina F; Meng, Melissa; White, Elizabeth A; Münger, Karl; Howley, Peter M; Meyers, Craig; Gack, Michaela U

    2018-03-15

    Retinoic acid-inducible gene I (RIG-I) is a key pattern recognition receptor that senses viral RNA and interacts with the mitochondrial adaptor MAVS, triggering a signaling cascade that results in the production of type I interferons (IFNs). This signaling axis is initiated by K63-linked ubiquitination of RIG-I mediated by the E3 ubiquitin ligase TRIM25, which promotes the interaction of RIG-I with MAVS. USP15 was recently identified as an upstream regulator of TRIM25, stabilizing the enzyme through removal of degradative K48-linked polyubiquitin, ultimately promoting RIG-I-dependent cytokine responses. Here, we show that the E6 oncoprotein of human papillomavirus type 16 (HPV16) as well as of other HPV types form a complex with TRIM25 and USP15 in human cells. In the presence of E6, the K48-linked ubiquitination of TRIM25 was markedly increased, and in line with this, TRIM25 degradation was enhanced. Our results further showed that E6 inhibited the TRIM25-mediated K63-linked ubiquitination of RIG-I and its CARD-dependent interaction with MAVS. HPV16 E6, but not E7, suppressed the RIG-I-mediated induction of IFN-β, chemokines, and IFN-stimulated genes (ISGs). Finally, CRISPR-Cas9 gene targeting in human keratinocytes showed that the TRIM25-RIG-I-MAVS triad is important for eliciting an antiviral immune response to HPV16 infection. Our study thus identifies a novel immune escape mechanism that is conserved among different HPV strains and further indicates that the RIG-I signaling pathway plays an important role in the innate immune response to HPV infection. IMPORTANCE Persistent infection and tumorigenesis by HPVs are known to require viral manipulation of a variety of cellular processes, including those involved in innate immune responses. Here, we show that the HPV E6 oncoprotein antagonizes the activation of the cytoplasmic innate immune sensor RIG-I by targeting its upstream regulatory enzymes TRIM25 and USP15. We further show that the RIG-I signaling cascade is important for an antiviral innate immune response to HPV16 infection, providing evidence that RIG-I, whose role in sensing RNA virus infections has been well characterized, also plays a crucial role in the antiviral host response to small DNA viruses of the Papillomaviridae family. Copyright © 2018 American Society for Microbiology.

  1. MSL/Mastcam Multispectral Observations of Lower Mt. Sharp Units: Spectral Evidence of Distinct Alteration Environments

    NASA Astrophysics Data System (ADS)

    Wellington, D. F.; Bell, J. F., III; Johnson, J. R.; Fraeman, A. A.; Kinch, K. M.; Godber, A.; Rice, M. S.

    2016-12-01

    The Mars Science Laboratory Curiosity rover reached the lower units of Mt. Sharp in Gale Crater approximately two years ago. Along the traverse, Mastcam multispectral observations have documented the visible/near-IR spectral variability of drill tailings, bedrock, float rocks, fines, and other materials, recording a set of diverse reflectance properties in twelve unique filters over wavelengths 400-1100 nm. The most recent multi-filter images include new spectral diversity not encountered in near-field imaging acquired earlier in the mission. Since departing Marias Pass ( sol 1072), the rover has sampled material from the Stimson sandstone unit four times at two widely separated locations. These drill pairs were designed to investigate alteration regions visible as bright haloes bordering fractures in the bedrock. Drill fines and piles of dumped sample material from these sites (at Bridger Basin and on the Naukluft Plateau) were targeted for multispectral observations, which quantify the differences in overall reflectance and spectral shape between the unaltered Stimson material and the light-toned haloes. In the latter, high reflectances and relatively flat spectral shapes are consistent with interpretations of silica enrichment from other instruments. Mastcam spectra of the portions of the underlying Murray Formation (mudstone) that were encountered on first approach to the Bagnold dunes, and again upon exiting the Naukluft Plateau, are consistent with the presence of crystalline hematite. Variations in the relative strength of hematite absorption features in different locations may suggest possible differences in relative and/or absolute abundances of hematite of varying degrees of crystallinity. Dune materials have low reflectances with a broad, shallow absorption near 1-µm consistent with an olivine-bearing basaltic composition. We present these and other examples of spectral variability encountered by the rover during its ascent up the lower slopes of Mt. Sharp.

  2. Ocean Observations Below Petermann Gletscher Ice Shelf, Greenland From a Cabled Observatory

    NASA Astrophysics Data System (ADS)

    Muenchow, A.; Nicholls, K. W.; Padman, L.; Washam, P.

    2016-12-01

    Petermann Gletscher in North Greenland features the second largest floating ice shelf by area in the northern hemisphere. In August of 2015 we drilled three holes through the ice shelf and deployed ocean sensors between 5 and 700 m below the glacier-ocean interface. The sensors are controlled by data loggers at the surface that also support a weather station and GPS. All data are transmitted near real-time via a satellite communication link that allowed data downloads and software uploads until February 2016. The system provided gap-free hourly data through the polar night with air temperatures dropping below -48 °C. Mean glacier speeds in winter (Nov.-Feb) were 1180±18 m/year; these values are 12±5% larger than previously reported winter speeds at this location. Hourly ocean observations revealed large bi-monthly pulses within 30 m of the glacier-ocean interface and amplitudes that exceed 1 °C in temperature and 1 psu in salinity. We posit that episodic discharge of glacial meltwater, modulated by the spring-neap tidal cycle thickens the boundary layer under the ice shelf at the location of our measurements. All data are posted at http://ows.udel.edu . A site visit is planned for August 2016 to fix communication failures, retrieve locally stored data, add sensors, and evaluate sustainability of this first cabled observatory on a floating and rapidly melting Greenland glacier.

  3. Reconfigurable Image Generator

    NASA Technical Reports Server (NTRS)

    Archdeacon, John L. (Inventor); Iwai, Nelson H. (Inventor); Kato, Kenji H. (Inventor); Sweet, Barbara T. (Inventor)

    2017-01-01

    A RiG may simulate visual conditions of a real world environment, and generate the necessary amount of pixels in a visual simulation at rates up to 120 frames per second. RiG may also include a database generation system capable of producing visual databases suitable to drive the visual fidelity required by the RiG.

  4. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Suchanecki, Z.; Antoniou, I.; Tasaki, S.

    We consider the problem of rigging for the Koopman operators of the Renyi and the baker maps. We show that the rigged Hilbert space for the Renyi maps has some of the properties of a strict inductive limit and give a detailed description of the rigged Hilbert space for the baker maps. {copyright} {ital 1996 American Institute of Physics.}

  5. 29 CFR 1926.1431 - Hoisting personnel.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... hook, load line and rigging) must not exceed 50 percent of the rated capacity for the radius and... pursuant to paragraph (b)(2) of this section, the total load (including the hook, load line, rigging and... number required to perform the work, whichever is less. (g) Attachment and rigging. (1) Hooks and other...

  6. 49 CFR 229.65 - Spring rigging.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

  7. 49 CFR 229.65 - Spring rigging.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

  8. 49 CFR 229.65 - Spring rigging.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

  9. 49 CFR 229.65 - Spring rigging.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

  10. 49 CFR 229.65 - Spring rigging.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Spring rigging. 229.65 Section 229.65....65 Spring rigging. (a) Protective construction or safety hangers shall be provided to prevent spring planks, spring seats or bolsters from dropping to track structure in event of a hanger or spring failure...

  11. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

  12. 16 CFR Figure 1 to Part 1512 - Bicycle Front Fork Cantilever Bending Test Rig

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Bicycle Front Fork Cantilever Bending Test Rig 1 Figure 1 to Part 1512 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS... Fork Cantilever Bending Test Rig EC03OC91.070 ...

  13. Injecting rabies immunoglobulin (RIG) into wounds only: A significant saving of lives and costly RIG.

    PubMed

    Bharti, Omesh Kumar; Madhusudana, Shampur Narayan; Wilde, Henry

    2017-04-03

    An increasing number of dog bite victims were being presented to public hospitals in Himachal Pradesh in 2014 amidst virtual non availability of any rabies immunoglobulin (RIG). Only a small quantity of equine rabies immunoglobulin (eRIG) was available from the government owned Central Research Institute (CRI) Kasauli. This available eRIG was used in 269 patients as an emergency response and only for local infiltration of severe bite wounds by suspected rabid dogs. This was followed by rabies vaccination, using the WHO approved intra-dermal Thai Red Cross Society vaccination schedule. A subgroup of 26 patients were later identified who had been severely bitten by laboratory confirmed rabid dogs. They were followed for more than one year and all were found to be alive.

  14. Citizen science data suggest that a novel rig improves landing rate and reduces injury and handling time in recreational angling with artificial lures in Baltic pike (Esox lucius).

    PubMed

    Bursell, Jens Jakob; Arlinghaus, Robert

    2018-01-01

    The optimal terminal gear in hook-and-line recreational fishing maximizes landing rates and minimizes injury to the fish because some fish will be released after capture. We designed a novel rig configuration in artificial lure fishing for top predators and examined its effectiveness in angling for Baltic northern pike ( Esox lucius ) using a citizen science approach based on observational data collected from volunteer anglers in the field. The novel rig included two changes to traditional rig designs common to artificial lure angling. First, hooks were mounted in a way giving better hook exposure and eliminating lever-arm effects from the lure to the hooks once a fish is hooked. This construction allowed the second change, being a shift to hooks 4-5 sizes smaller than those used on traditional hook mounts. We analysed observational data collected by volunteer anglers using either the novel rig or a standard rig mount in two types of artificial lures (softbait and hardbait) of the same size (about 17 cm). Using N  = 768 pike contacts as input data, we showed the landing rates of pike targeted with artificial lures significantly and substantially increased from 45% with normal-rigs to 85% when the same lure types were fished with the novel rig configuration. Lure type and water temperature had no effects on landing rates. Moreover, hardbaits on normal-rigs produced significantly more injury, bleeding and elevated unhooking time compared to fish captured on hardbaits with release-rigs. We conclude that simple changes to traditional hook sizes and mounts in lure fishing may benefit both anglers and the fishes that are to be released and that citizen science projects with volunteer anglers are able to provide good data in proof-of-concept studies. Further experimental studies are needed to differentiate hook size from hook mount effects because both variables were confounded in the results of the observational data presented here.

  15. Design and commission of an experimental test rig to apply a full-scale pressure load on composite sandwich panels representative of an aircraft secondary structure

    NASA Astrophysics Data System (ADS)

    Crump, D. A.; Dulieu-Barton, J. M.; Savage, J.

    2010-01-01

    This paper describes the design of a test rig, which is used to apply a representative pressure load to a full-scale composite sandwich secondary aircraft structure. A generic panel was designed with features to represent those in the composite sandwich secondary aircraft structure. To provide full-field strain data from the panels, the test rig was designed for use with optical measurement techniques such as thermoelastic stress analysis (TSA) and digital image correlation (DIC). TSA requires a cyclic load to be applied to a structure for the measurement of the strain state; therefore, the test rig has been designed to be mounted on a standard servo-hydraulic test machine. As both TSA and DIC require an uninterrupted view of the surface of the test panel, an important consideration in the design is facilitating the optical access for the two techniques. To aid the test rig design a finite element (FE) model was produced. The model provides information on the deflections that must be accommodated by the test rig, and ensures that the stress and strain levels developed in the panel when loaded in the test rig would be sufficient for measurement using TSA and DIC. Finally, initial tests using the test rig have shown it to be capable of achieving the required pressure and maintaining a cyclic load. It was also demonstrated that both TSA and DIC data can be collected from the panels under load, which are used to validate the stress and deflection derived from the FE model.

  16. Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon–independent apoptosis in human melanoma cells

    PubMed Central

    Besch, Robert; Poeck, Hendrik; Hohenauer, Tobias; Senft, Daniela; Häcker, Georg; Berking, Carola; Hornung, Veit; Endres, Stefan; Ruzicka, Thomas; Rothenfusser, Simon; Hartmann, Gunther

    2009-01-01

    The retinoic acid–inducible gene I (RIG-I) and melanoma differentiation–associated antigen 5 (MDA-5) helicases sense viral RNA in infected cells and initiate antiviral responses such as the production of type I IFNs. Here we have shown that RIG-I and MDA-5 also initiate a proapoptotic signaling pathway that is independent of type I IFNs. In human melanoma cells, this signaling pathway required the mitochondrial adapter Cardif (also known as IPS-1) and induced the proapoptotic BH3-only proteins Puma and Noxa. RIG-I– and MDA-5–initiated apoptosis required Noxa but was independent of the tumor suppressor p53. Triggering this pathway led to efficient activation of mitochondrial apoptosis, requiring caspase-9 and Apaf-1. Surprisingly, this proapoptotic signaling pathway was also active in nonmalignant cells, but these cells were much less sensitive to apoptosis than melanoma cells. Endogenous Bcl-xL rescued nonmalignant, but not melanoma, cells from RIG-I– and MDA-5–mediated apoptosis. In addition, we confirmed the results of the in vitro studies, demonstrating that RIG-I and MDA-5 ligands both reduced human tumor lung metastasis in immunodeficient NOD/SCID mice. These results identify an IFN-independent antiviral signaling pathway initiated by RIG-I and MDA-5 that activates proapoptotic signaling and, unless blocked by Bcl-xL, results in apoptosis. Due to their immunostimulatory and proapoptotic activity, RIG-I and MDA-5 ligands have therapeutic potential due to their ability to overcome the characteristic resistance of melanoma cells to apoptosis. PMID:19620789

  17. 29 CFR 1926.753 - Hoisting and rigging.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... lift rigging procedure. (1) A multiple lift shall only be performed if the following criteria are met: (i) A multiple lift rigging assembly is used; (ii) A maximum of five members are hoisted per lift... multiple lift have been trained in these procedures in accordance with § 1926.761(c)(1). (v) No crane is...

  18. Long-term ground-water monitoring program and performance-evaluation plan for the extraction system at the former Nike Missile Battery Site, Aberdeen Proving Ground, Maryland

    USGS Publications Warehouse

    Senus, Michael P.; Tenbus, Frederick J.

    2000-01-01

    This report presents lithologic and ground-water-quality data collected during April and May 2000 in the remote areas of the tidal wetland of West Branch Canal Creek, Aberdeen Proving Ground, Maryland. Contamination of the Canal Creek aquifer with volatile organic compounds has been documented in previous investigations of the area. This study was conducted to investigate areas that were previously inaccessible because of deep mud and shallow water, and to support ongoing investigations of the fate and transport of volatile organic compounds in the Canal Creek aquifer. A unique vibracore drill rig mounted on a hovercraft was used for drilling and ground-water sampling. Continuous cores of the wetland sediment and of the Canal Creek aquifer were collected at five sites. Attempts to sample ground water were made by use of a continuous profiler at 12 sites, without well installation, at a total of 81 depths within the aquifer. Of those 81 attempts, only 34 sampling depths produced enough water to collect samples. Ground-water samples from two sites had the highest concentrations of volatile organic compounds?with total volatile organic compound concentrations in the upper part of the aquifer ranging from about 15,000 to 50,000 micrograms per liter. Ground-water samples from five sites had much lower total volatile organic compound concentrations (95 to 2,100 micrograms per liter), whereas two sites were essentially not contaminated, with total volatile organic compound concentrations less than or equal to 5 micrograms per liter.

  19. Fatalities among oil and gas extraction workers--United States, 2003-2006.

    PubMed

    2008-04-25

    Oil and gas extraction (i.e., removing oil and natural gas from the ground) is a growing industry in the United States, employing approximately 380,000 workers in 2006. In recent years, activity in this industry has increased substantially, from an average of 800 actively drilling rigs in the United States during the 1990s to approximately 1,300 during 2003-2006. In August 2005, the U.S. Department of Labor's Bureau of Labor Statistics (BLS) asked CDC to investigate a 15% increase in fatalities among oil and gas extraction workers (from 85 fatalities in 2003 to 98 in 2004). CDC analyzed data from the BLS Census of Fatal Occupational Injuries (CFOI) for the period 2003-2006. This report describes the results of that analysis, which indicated that increases in oil and gas extraction activity were correlated with an increase in the rate of fatal occupational injuries in this industry, with an annual fatality rate of 30.5 per 100,000 workers (404 fatalities) during 2003-2006, approximately seven times the rate for all workers (4.0 per 100,000 workers). Nearly half of all fatal injuries among these workers were attributed to highway motor-vehicle crashes and workers being struck by machinery or equipment. Employers should work with existing industry groups and federal, state, and local government agencies to promote seatbelt use. In addition, researchers and public health officials should collaborate with industry groups to establish engineering and process controls that remove workers from potentially dangerous machinery while drilling and servicing oil and gas wells.

  20. Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway.

    PubMed

    Sanchez, Jacint G; Chiang, Jessica J; Sparrer, Konstantin M J; Alam, Steven L; Chi, Michael; Roganowicz, Marcin D; Sankaran, Banumathi; Gack, Michaela U; Pornillos, Owen

    2016-08-02

    Antiviral response pathways induce interferon by higher-order assembly of signaling complexes called signalosomes. Assembly of the RIG-I signalosome is regulated by K63-linked polyubiquitin chains, which are synthesized by the E3 ubiquitin ligase, TRIM25. We have previously shown that the TRIM25 coiled-coil domain is a stable, antiparallel dimer that positions two catalytic RING domains on opposite ends of an elongated rod. We now show that the RING domain is a separate self-association motif that engages ubiquitin-conjugated E2 enzymes as a dimer. RING dimerization is required for catalysis, TRIM25-mediated RIG-I ubiquitination, interferon induction, and antiviral activity. We also provide evidence that RING dimerization and E3 ligase activity are promoted by binding of the TRIM25 SPRY domain to the RIG-I effector domain. These results indicate that TRIM25 actively participates in higher-order assembly of the RIG-I signalosome and helps to fine-tune the efficiency of the RIG-I-mediated antiviral response. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  1. Comparative analysis of viral RNA signatures on different RIG-I-like receptors

    PubMed Central

    Sanchez David, Raul Y; Combredet, Chantal; Sismeiro, Odile; Dillies, Marie-Agnès; Jagla, Bernd; Coppée, Jean-Yves; Mura, Marie; Guerbois Galla, Mathilde; Despres, Philippe; Tangy, Frédéric; Komarova, Anastassia V

    2016-01-01

    The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown. To decipher the viral RNA signature on RLRs during viral infection, we tagged RLRs (RIG-I, MDA5, LGP2) and applied tagged protein affinity purification followed by next-generation sequencing (NGS) of associated RNA molecules. Two viruses with negative- and positive-sense RNA genome were used: measles (MV) and chikungunya (CHIKV). NGS analysis revealed that distinct regions of MV genome were specifically recognized by distinct RLRs: RIG-I recognized defective interfering genomes, whereas MDA5 and LGP2 specifically bound MV nucleoprotein-coding region. During CHIKV infection, RIG-I associated specifically to the 3’ untranslated region of viral genome. This study provides the first comparative view of the viral RNA ligands for RIG-I, MDA5 and LGP2 in the presence of infection. DOI: http://dx.doi.org/10.7554/eLife.11275.001 PMID:27011352

  2. Truncation of the C-terminal region of Toscana Virus NSs protein is critical for interferon-β antagonism and protein stability.

    PubMed

    Gori Savellini, Gianni; Gandolfo, Claudia; Cusi, Maria Grazia

    2015-12-01

    Toscana Virus (TOSV) is a Phlebovirus responsible for central nervous system (CNS) injury in humans. The TOSV non-structural protein (NSs), which interacting with RIG-I leads to its degradation, was analysed in the C terminus fragment in order to identify its functional domains. To this aim, two C-terminal truncated NSs proteins, Δ1C-NSs (aa 1-284) and Δ2C-NSs (aa 1-287) were tested. Only Δ1C-NSs did not present any inhibitory effect on RIG-I and it showed a greater stability than the whole NSs protein. Moreover, the deletion of the TLQ aa sequence interposed between the two ΔC constructs caused a greater accumulation of the protein with a weak inhibitory effect on RIG-I, indicating some involvement of these amino acids in the NSs activity. Nevertheless, all the truncated proteins were still able to interact with RIG-I, suggesting that the domains responsible for RIG-I signaling and RIG-I interaction are mapped on different regions of the protein. Copyright © 2015 Elsevier Inc. All rights reserved.

  3. Dynamic Spin Rig Upgraded With a Five- Axis-Controlled Three-Magnetic-Bearing Support System With Forward Excitation

    NASA Technical Reports Server (NTRS)

    Morrison, Carlos R.; Mehmed, Oral

    2003-01-01

    The NASA Glenn Research Center Dynamic Spin Rig is used for experimental evaluation of vibration analysis methods and dynamic characteristics for rotating systems. Measurements are made while rotors are spun and vibrated in a vacuum chamber. The rig has been upgraded with a new active magnetic bearing rotor support and excitation system. This design is expected to provide operational improvements over the existing rig. The rig will be able to be operated in either the old or new configuration. In the old configuration, two ball bearings support the vertical shaft of the rig, with the test article located between the bearings. Because the bearings operate in a vacuum, lubrication is limited to grease. This limits bearing life and speed. In addition, the old configuration employs two voice-coil electromagnetic shakers to apply oscillatory axial forces or transverse moments to the rotor shaft through a thrust bearing. The excitation amplitudes that can be imparted to the test article with this system are not adequate for components that are highly damped. It is expected that the new design will overcome these limitations.

  4. Validation of the Small Hot Jet Acoustic Rig for Jet Noise Research

    NASA Technical Reports Server (NTRS)

    Bridges, James; Brown, Clifford A.

    2005-01-01

    The development and acoustic validation of the Small Hot Jet Aeroacoustic Rig (SHJAR) is documented. Originally conceived to support fundamental research in jet noise, the rig has been designed and developed using the best practices of the industry. While validating the rig for acoustic work, a method of characterizing all extraneous rig noise was developed. With this in hand, the researcher can know when the jet data being measured is being contaminated and design the experiment around this limitation. Also considered is the question of uncertainty, where it is shown that there is a fundamental uncertainty of 0.5dB or so to the best experiments, confirmed by repeatability studies. One area not generally accounted for in the uncertainty analysis is the variation which can result from differences in initial condition of the nozzle shear layer. This initial condition was modified and the differences in both flow and sound were documented. The bottom line is that extreme caution must be applied when working on small jet rigs, but that highly accurate results can be made independent of scale.

  5. Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway

    DOE PAGES

    Sanchez, Jacint G.; Chiang, Jessica J.; Sparrer, Konstantin M. J.; ...

    2016-07-14

    Antiviral response pathways induce interferon by higher-order assembly of signaling complexes called signalosomes. Assembly of the RIG-I signalosome is regulated by K63-linked polyubiquitin chains, which are synthesized by the E3 ubiquitin ligase, TRIM25. We have previously shown that the TRIM25 coiled-coil domain is a stable, antiparallel dimer that positions two catalytic RING domains on opposite ends of an elongated rod. We now show that the RING domain is a separate self-association motif that engages ubiquitin-conjugated E2 enzymes as a dimer. RING dimerization is required for catalysis, TRIM25-mediated RIG-I ubiquitination, interferon induction, and antiviral activity. We also provide evidence that RINGmore » dimerization and E3 ligase activity are promoted by binding of the TRIM25 SPRY domain to the RIG-I effector domain. These results indicate that TRIM25 actively participates in higher-order assembly of the RIG-I signalosome and helps to fine-tune the efficiency of the RIG-I-mediated antiviral response.« less

  6. Kinematic perturbation in the flexion-extension axis for two lumbar rigs during a high impact jump task.

    PubMed

    Portus, Marc R; Lloyd, David G; Elliott, Bruce C; Trama, Neil L

    2011-05-01

    The measurement of lumbar spine motion is an important step for injury prevention research during complex and high impact activities, such as cricket fast bowling or javelin throwing. This study examined the performance of two designs of a lumbar rig, previously used in gait research, during a controlled high impact bench jump task. An 8-camera retro-reflective motion analysis system was used to track the lumbar rig. Eleven athletes completed the task wearing the two different lumbar rig designs. Flexion extension data were analyzed using a fast Fourier transformation to assess the signal power of these data during the impact phase of the jump. The lumbar rig featuring an increased and pliable base of support recorded moderately less signal power through the 0-60 Hz spectrum, with statistically less magnitudes at the 0-5 Hz (p = .039), 5-10 Hz (p = .005) and 10-20 Hz (p = .006) frequency bins. A lumbar rig of this design would seem likely to provide less noisy lumbar motion data during high impact tasks.

  7. Mutual antagonism between the Ebola virus VP35 protein and the RIG-I activator PACT determines infection outcome.

    PubMed

    Luthra, Priya; Ramanan, Parameshwaran; Mire, Chad E; Weisend, Carla; Tsuda, Yoshimi; Yen, Benjamin; Liu, Gai; Leung, Daisy W; Geisbert, Thomas W; Ebihara, Hideki; Amarasinghe, Gaya K; Basler, Christopher F

    2013-07-17

    The cytoplasmic pattern recognition receptor RIG-I is activated by viral RNA and induces type I IFN responses to control viral replication. The cellular dsRNA binding protein PACT can also activate RIG-I. To counteract innate antiviral responses, some viruses, including Ebola virus (EBOV), encode proteins that antagonize RIG-I signaling. Here, we show that EBOV VP35 inhibits PACT-induced RIG-I ATPase activity in a dose-dependent manner. The interaction of PACT with RIG-I is disrupted by wild-type VP35, but not by VP35 mutants that are unable to bind PACT. In addition, PACT-VP35 interaction impairs the association between VP35 and the viral polymerase, thereby diminishing viral RNA synthesis and modulating EBOV replication. PACT-deficient cells are defective in IFN induction and are insensitive to VP35 function. These data support a model in which the VP35-PACT interaction is mutually antagonistic and plays a fundamental role in determining the outcome of EBOV infection. Copyright © 2013 Elsevier Inc. All rights reserved.

  8. Emerging Role of Ubiquitination in Antiviral RIG-I Signaling

    PubMed Central

    Maelfait, Jonathan

    2012-01-01

    Summary: Detection of viruses by the innate immune system involves the action of specialized pattern recognition receptors. Intracellular RIG-I receptors sense the presence of viral nucleic acids in infected cells and trigger signaling pathways that lead to the production of proinflammatory and antiviral proteins. Over the past few years, posttranslational modification of RIG-I and downstream signaling proteins by different types of ubiquitination has been found to be a key event in the regulation of RIG-I-induced NF-κB and interferon regulatory factor 3 (IRF3) activation. Multiple ubiquitin ligases, deubiquitinases, and ubiquitin binding scaffold proteins contribute to both positive and negative regulation of the RIG-I-induced antiviral immune response. A better understanding of the function and activity of these proteins might eventually lead to the development of novel therapeutic approaches for management of viral diseases. PMID:22390971

  9. Molecular characterisation of RIG-I-like helicases in the black flying fox, Pteropus alecto.

    PubMed

    Cowled, Christopher; Baker, Michelle L; Zhou, Peng; Tachedjian, Mary; Wang, Lin-Fa

    2012-04-01

    The RIG-I like helicases, RIG-I, mda5 and LGP2 are an evolutionarily conserved family of cytosolic pattern recognition receptors important in the recognition of viral RNA, and responsible for the innate induction of interferons and proinflammatory cytokines upon viral infection. Bats are natural reservoir hosts to a variety of RNA viruses that cause significant morbidity and mortality in other species; however the mechanisms responsible for the control of viral replication in bats are not understood. This report describes the molecular cloning and expression analysis of RIG-I, mda5 and LGP2 genes in the fruit bat Pteropus alecto, and is the first description of RIG-I like helicases from any species of bat. Our results demonstrate that P. alecto RIG-I, mda5 and LGP2 have similar primary structures and tissue expression patterns to their counterparts in humans and other mammals. Stimulation of bat kidney cells with synthetic dsRNA (poly I:C) induced high levels of interferon β and rapid upregulation of all three helicases. These findings reveal that the cytoplasmic virus sensing machinery is present and intact in P. alecto. This study provides the foundation for further investigations into the interactions between bat RIG-I-like helicases and viruses to elucidate the mechanisms responsible for the asymptomatic nature of viral infections in bats. Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.

  10. A method for testing railway wheel sets on a full-scale roller rig

    NASA Astrophysics Data System (ADS)

    Liu, Binbin; Bruni, Stefano

    2015-09-01

    Full-scale roller rigs for tests on a single axle enable the investigation of several dynamics and durability problems related with the design and operation of the railway rolling stock. In order to exploit the best potential of this test equipment, appropriate test procedures need to be defined, particularly in terms of actuators' references, to make sure that meaningful wheel -rail contact conditions can be reproduced. The aim of this paper is to propose a new methodology to define the forces to be generated by the actuators in the rig in order to best reproduce the behaviour of a wheel set and especially the wheel -rail contact forces in a running condition of interest as obtained either from multi-body system (MBS) simulation or from on-track measurements. The method is supported by the use of a mathematical model of the roller rig and uses an iterative correction scheme, comparing the time histories of the contact force components from the roller rig test as predicted by the mathematical model to a set of target contact force time histories. Two methods are introduced, the first one considering a standard arrangement of the roller rig, the second one assuming that a differential gear is introduced in the rig, allowing different rolling speeds of the two rollers. Results are presented showing that the deviation of the roller rig test results from the considered targets can be kept within low tolerances (1% approximately) as far as the vertical and lateral contact forces on both wheels are concerned. For the longitudinal forces, larger deviations are obtained except in the case where a differential gear is introduced.

  11. Development of a low cost test rig for standalone WECS subject to electrical faults.

    PubMed

    Himani; Dahiya, Ratna

    2016-11-01

    In this paper, a contribution to the development of low-cost wind turbine (WT) test rig for stator fault diagnosis of wind turbine generator is proposed. The test rig is developed using a 2.5kW, 1750 RPM DC motor coupled to a 1.5kW, 1500 RPM self-excited induction generator interfaced with a WT mathematical model in LabVIEW. The performance of the test rig is benchmarked with already proven wind turbine test rigs. In order to detect the stator faults using non-stationary signals in self-excited induction generator, an online fault diagnostic technique of DWT-based multi-resolution analysis is proposed. It has been experimentally proven that for varying wind conditions wavelet decomposition allows good differentiation between faulty and healthy conditions leading to an effective diagnostic procedure for wind turbine condition monitoring. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

  12. Regenerative Fuel Cell Test Rig at Glenn Research Center

    NASA Technical Reports Server (NTRS)

    Chang, Bei-Jiann; Johnson, Donald W.; Garcia, Christopher P.; Jakupca, Ian J.; Scullin, Vincent J.; Bents, David J.

    2003-01-01

    The regenerative fuel cell development effort at Glenn Research Center (GRC) involves the integration of a dedicated fuel cell and electrolyzer into an energy storage system test rig. The test rig consists of a fuel cell stack, an electrolysis stack, cooling pumps, a water transfer pump, gas recirculation pumps, phase separators, storage tanks for oxygen (O2) and hydrogen (H2), heat exchangers, isolation valves, pressure regulators, interconnecting tubing, nitrogen purge provisions, and instrumentation for control and monitoring purposes. The regenerative fuel cell (RFC) thus formed is a completely closed system which is capable of autonomous cyclic operation. The test rig provides direct current (DC) load and DC power supply to simulate power consumption and solar power input. In addition, chillers are used as the heat sink to dissipate the waste heat from the electrochemical stack operation. Various vents and nitrogen (N2) sources are included in case inert purging is necessary to safe the RFC test rig.

  13. NASA Lewis Research Center lean-, rich-burn materials test burner rig

    NASA Technical Reports Server (NTRS)

    Stearns, C. A.; Robinson, R. C.

    1994-01-01

    The lean-, rich-burn materials test burner rig at NASA LeRC is used to evaluate the high temperature environmental durability of aerospace materials. The rig burns jet fuel and pressurized air, and sample materials can be subjected to both lean-burn and rich-burn environments. As part of NASA's Enabling Propulsion Materials (EPM) program, an existing rig was adapted to simulate the rich-burn quick-quench lean-burn (RQL) combustor concept which is being considered for the HSCT (high speed civil transport) aircraft. RQL materials requirements exceed that of current superalloys, thus ceramic matrix composites (CMC's) emerged as the leading candidate materials. The performance of these materials in the quasi reducing environment of the rich-burn section of the RQL is of fundamental importance to materials development. This rig was developed to conduct such studies, and its operation and capabilities are described.

  14. Advanced Control Surface Seal Development at NASA GRC for Future Space Launch Vehicles

    NASA Technical Reports Server (NTRS)

    Dunlap, Patrick H., Jr.; Steinetz, Bruce M.; DeMange, Jeffrey J.

    2003-01-01

    NASA s Glenn Research Center (GRC) is developing advanced control surface seal technologies for future space launch vehicles as part of the Next Generation Launch Technology project (NGLT). New resilient seal designs are currently being fabricated and high temperature seal preloading devices are being developed as a means of improving seal resiliency. GRC has designed several new test rigs to simulate the temperatures, pressures, and scrubbing conditions that seals would have to endure during service. A hot compression test rig and hot scrub test rig have been developed to perform tests at temperatures up to 3000 F. Another new test rig allows simultaneous seal flow and scrub tests at room temperature to evaluate changes in seal performance with scrubbing. These test rigs will be used to evaluate the new seal designs. The group is also performing tests on advanced TPS seal concepts for Boeing using these new test facilities.

  15. A New High-Speed Oil-Free Turbine Engine Rotordynamic Simulator Test Rig

    NASA Technical Reports Server (NTRS)

    Howard, Samuel A.

    2007-01-01

    A new test rig has been developed for simulating high-speed turbomachinery rotor systems using Oil-Free foil air bearing technology. Foil air bearings have been used in turbomachinery, primarily air cycle machines, for the past four decades to eliminate the need for oil lubrication. The goal of applying this bearing technology to other classes of turbomachinery has prompted the fabrication of this test rig. The facility gives bearing designers the capability to test potential bearing designs with shafts that simulate the rotating components of a target machine without the high cost of building "make-and-break" hardware. The data collected from this rig can be used to make design changes to the shaft and bearings in subsequent design iterations. This paper describes the new test rig and demonstrates its capabilities through the initial run with a simulated shaft system.

  16. A review of turbulent-boundary-layer heat transfer research at Stanford, 1958-1983

    NASA Technical Reports Server (NTRS)

    Moffat, R. J.; Kays, W. M.

    1984-01-01

    For the past 25 years, there has existed in the Thermosciences Laboratory of the Mechanical Engineering Department of Stanford University a research program, primarily experimental, concerned with heat transfer through turbulent boundary layers. In the early phases of the program, the topics considered were the simple zero-pressure-gradient turbulent boundary layer with constant and with varying surface temperature, and the accelerated boundary layer. Later equilibrium boundary layers were considered along with factors affecting the boundary layer, taking into account transpired flows, flows with axial pressure gradients, transpiration, acceleration, deceleration, roughness, full-coverage film cooling, surface curvature, free convection, and mixed convection. A description is provided of the apparatus and techniques used, giving attention to the smooth plate rig, the rough plate rig, the full-coverage film cooling rig, the curvature rig, the concave wall rig, the mixed convection tunnel, and aspects of data reduction and uncertainty analysis.

  17. Ocean observations from below Petermann Gletscher

    NASA Astrophysics Data System (ADS)

    Muenchow, A.; Nicholls, K. W.; Heuzé, C.; Wahlin, A.; Mix, A. C.

    2015-12-01

    Petermann Gletscher drains 4% of the Greenland ice sheet via a floating ice shelf that has shrunk from 1,300 to 900 km^2 in area via two calving events in 2010 and 2012. The glacier is thinning by about 10 vertical meters per year when integrated over 45 km from the grounding zone to the terminus. Most of this mass loss is caused by ocean melting, but only a single vertical ocean profile taken in 2002 exists. The fjord was mostly free of sea ice in August when we visited in 2003, 2006, 2007, 2009, and 2012 and noticed a small warming trend of bottom waters. During a 2-day survey of Petermann Fjord and adjacent Nares Strait in 2012 we documented a large intrusion of warmer Atlantic waters spilling over the 400 m deep sill and sinking to more than 800 m depth. These waters fill the deep basin of the fjord and move towards the grounding zone of glacier at 550 m below the sea surface. In August 2015 the Swedish icebreaker I/B Oden is scheduled to enter Nares Strait and Petermann Fjord to support field work on land, on water, and on the floating glacier. We here report preliminary results from both ocean surveys and ice shelf moorings. The moored observations from under the ice shelf extend synoptic survey data from Oden. The ice shelf moorings are designed to resolve tidal to interannual variations of water properties under the floating glacier. More specifically, we plan to install a total 13 discrete sensors to measure ocean temperature, salinity, and pressure at five locations distributed both along and across the floating glacier. Hot water drilling provides the holes through the 200 to 500 m thick glacier ice to collect sediment cores, take a profile of temperature and salinity, and deploy two to five cabled sensors per mooring. If successful, data from these cabled instruments will be distributed via surface Iridium connections and posted on the web in near real time. We will discuss successes and failures of this ambitious and high risk program that was facilitated by a bottom-up collaboration of British, Swedish, and US investigators and their respective funding agencies all working on very short and tense schedules. Figure: Sketch of mooring placement on a map (left panel) with 2014 flight tracks and glacier profiles (right panel) obtained from laser altimeter data along the tracks.

  18. Laboratory facility for testing electric-vehicle batteries Test rig for simulating duty cycles with different discharge modes

    NASA Astrophysics Data System (ADS)

    Hamilton, J. A.; Rand, D. A. J.

    1983-03-01

    A test rig has been designed and constructed to examine the performance of batteries under laboratory conditions that simulate the power characteristics of electric vehicles. Each station in the rig subjects a battery to continuous charge/discharge cycles, with an equalising charge every eighth cycle. The battery discharge follows the current-verse-time profile of a given vehicle operating under a driving schedule normal to road service. The test rig allows both smooth- and pulsed-current discharge to be investigated. Data collection is accomplished either with multi-pen recorders or with a computer-based information logger.

  19. Regulation of WNT Signaling at the Neuromuscular Junction by the Immunoglobulin Superfamily Protein RIG-3 in Caenorhabditis elegans

    PubMed Central

    Pandey, Pratima; Bhardwaj, Ashwani; Babu, Kavita

    2017-01-01

    Perturbations in synaptic function could affect the normal behavior of an animal, making it important to understand the regulatory mechanisms of synaptic signaling. Previous work has shown that in Caenorhabditis elegans an immunoglobulin superfamily protein, RIG-3, functions in presynaptic neurons to maintain normal acetylcholine receptor levels at the neuromuscular junction (NMJ). In this study, we elucidate the molecular and functional mechanism of RIG-3. We demonstrate by genetic and BiFC (Bi-molecular Fluorescence Complementation) assays that presynaptic RIG-3 functions by directly interacting with the immunoglobulin domain of the nonconventional Wnt receptor, ROR receptor tyrosine kinase (RTK), CAM-1, which functions in postsynaptic body-wall muscles. This interaction in turn inhibits Wnt/LIN-44 signaling through the ROR/CAM-1 receptor, and allows for maintenance of normal acetylcholine receptor, AChR/ACR-16, levels at the neuromuscular synapse. Further, this work reveals that RIG-3 and ROR/CAM-1 function through the β-catenin/HMP-2 at the NMJ. Taken together, our results demonstrate that RIG-3 functions as an inhibitory molecule of the Wnt/LIN-44 signaling pathway through the RTK, CAM-1. PMID:28515212

  20. Large Eddy Simulations of the Tilted Rig Experiment: A Two-dimensional Rayleigh-Taylor Instability Case

    NASA Astrophysics Data System (ADS)

    Rollin, Bertrand; Denissen, Nicholas A.; Reisner, Jon M.; Andrews, Malcolm J.

    2012-11-01

    The tilted rig experiment is a derivative of the rocket rig experiment designed to investigate turbulent mixing induced by the Rayleigh-Taylor (RT) instability. A tank containing two fluids of different densities is accelerated downwards between two parallel guiding rods by rocket motors. The acceleration is such that the pressure and density gradients face opposite directions at the fluids interface, creating a Rayleigh-Taylor unstable configuration. The rig is tilted such that the tank is initially at an angle and the acceleration is not perpendicular to the fluids interface when the rockets fire. This results in a two dimensional Rayleigh-Taylor instability case where the fluids experience RT mixing and a bulk overturning motion. The tilted rig is therefore a valuable experiment to help calibrating two-dimensional mixing models. Large Eddy Simulations of the tilted rig experiments will be compared to available experimental results. A study of the behavior of turbulence variables relevant to turbulence modeling will be presented. LA-UR 12-23829. This work was performed for the U.S. Department of Energy by Los Alamos National Laboratory under Contract No.DEAC52- 06NA2-5396.

  1. Towards the control of the modal energy transfer in transverse mode instabilities

    NASA Astrophysics Data System (ADS)

    Stihler, Christoph; Jauregui, Cesar; Tünnermann, Andreas; Limpert, Jens

    2018-02-01

    Thermally-induced refractive index gratings (RIG) in high-power fiber laser systems lead to transverse mode instabilities (TMI) above a certain average power threshold. The effect of TMI is currently the main limitation for the further average power scaling of fiber lasers and amplifiers with nearly diffraction-limited beam quality. In this work we experimentally investigate, for the first time, the growth of the RIG strength by introducing a phase-shift between the RIG and the modal interference pattern in a fiber amplifier. The experiments reveal that the RIG is strong enough to couple energy between different transverse modes even at powers significantly below the TMI threshold, provided that the introduced phase-shift is high enough. This indicates that, as the strength of the RIG further increases with increasing average output power, the RIG becomes more and more sensitive to even small noise-induced phase-shifts, which ultimately trigger TMI. Furthermore, it is shown that a beam cleaning also occurs when a positive phase-shift is introduced, even above the TMI threshold. This finding will pave the way for the development of a new class of mitigation strategies for TMI, which key feature is the control of the introduced phase-shift.

  2. Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity.

    PubMed

    Chiang, Jessica J; Sparrer, Konstantin M J; van Gent, Michiel; Lässig, Charlotte; Huang, Teng; Osterrieder, Nikolaus; Hopfner, Karl-Peter; Gack, Michaela U

    2018-01-01

    The sensor RIG-I detects double-stranded RNA derived from RNA viruses. Although RIG-I is also known to have a role in the antiviral response to DNA viruses, physiological RNA species recognized by RIG-I during infection with a DNA virus are largely unknown. Using next-generation RNA sequencing (RNAseq), we found that host-derived RNAs, most prominently 5S ribosomal RNA pseudogene 141 (RNA5SP141), bound to RIG-I during infection with herpes simplex virus 1 (HSV-1). Infection with HSV-1 induced relocalization of RNA5SP141 from the nucleus to the cytoplasm, and virus-induced shutoff of host protein synthesis downregulated the abundance of RNA5SP141-interacting proteins, which allowed RNA5SP141 to bind RIG-I and induce the expression of type I interferons. Silencing of RNA5SP141 strongly dampened the antiviral response to HSV-1 and the related virus Epstein-Barr virus (EBV), as well as influenza A virus (IAV). Our findings reveal that antiviral immunity can be triggered by host RNAs that are unshielded following depletion of their respective binding proteins by the virus.

  3. Local infiltration of rabies immunoglobulins without systemic intramuscular administration: An alternative cost effective approach for passive immunization against rabies

    PubMed Central

    Bharti, Omesh Kumar; Madhusudana, Shampur Narayan; Gaunta, Pyare Lal; Belludi, Ashwin Yajaman

    2016-01-01

    ABSTRACT Presently the dose of rabies immunoglobulin (RIG) which is an integral part of rabies post exposure prophylaxis (PEP) is calculated based on body weight though the recommendation is to infiltrate the wound(s). This practice demands large quantities of RIG which may be unaffordable to many patients. In this background, we conducted this study to know if the quantity and cost of RIG can be reduced by restricting passive immunization to local infiltration alone and avoiding systemic intramuscular administration based on the available scientific evidence. Two hundred and sixty nine category III patients bitten by suspect or confirmed rabid dogs/animals were infiltrated with equine rabies immunoglobulin (ERIGs) in and around the wound. The quantity of ERIG used was proportionate to the size and number of wounds irrespective of their body weight. They were followed with a regular course of rabies vaccination by intra-dermal route. As against 363 vials of RIGs required for all these cases as per current recommendation based on body weight, they required only 42 vials of 5ml RIG. Minimum dose of RIGs given was 0.25 ml and maximum dose given was 8 ml. On an average 1.26 ml of RIGs was required per patient that costs Rs. 150 ($3). All the patients were followed for 9 months and they were healthy and normal at the end of observation period. With local infiltration, that required small quantities of RIG, the RIGs could be made available to all patients in times of short supply in the market. A total of 30 (11%) serum samples of patients were tested for rabies virus neutralizing antibodies by the rapid fluorescent focus inhibition test (RFFIT) and all showed antibody titers >0.5 IU/mL by day 14. In no case the dose was higher than that required based on body weight and no immunosuppression resulted. To conclude, this pilot study shows that local infiltration of RIG need to be considered in times of non-availability in the market or unaffordability by poor patients. This preliminary study needs to be done on larger scale in other centers with long term follow up to substantiate the results of our study. PMID:26317441

  4. The autoinhibitory CARD2-Hel2i Interface of RIG-I governs RNA selection.

    PubMed

    Ramanathan, Anand; Devarkar, Swapnil C; Jiang, Fuguo; Miller, Matthew T; Khan, Abdul G; Marcotrigiano, Joseph; Patel, Smita S

    2016-01-29

    RIG-I (Retinoic Acid Inducible Gene-I) is a cytosolic innate immune receptor that detects atypical features in viral RNAs as foreign to initiate a Type I interferon signaling response. RIG-I is present in an autoinhibited state in the cytoplasm and activated by blunt-ended double-stranded (ds)RNAs carrying a 5' triphosphate (ppp) moiety. These features found in many pathogenic RNAs are absent in cellular RNAs due to post-transcriptional modifications of RNA ends. Although RIG-I is structurally well characterized, the mechanistic basis for RIG-I's remarkable ability to discriminate between cellular and pathogenic RNAs is not completely understood. We show that RIG-I's selectivity for blunt-ended 5'-ppp dsRNAs is ≈3000 times higher than non-blunt ended dsRNAs commonly found in cellular RNAs. Discrimination occurs at multiple stages and signaling RNAs have high affinity and ATPase turnover rate and thus a high katpase/Kd. We show that RIG-I uses its autoinhibitory CARD2-Hel2i (second CARD-helicase insertion domain) interface as a barrier to select against non-blunt ended dsRNAs. Accordingly, deletion of CARDs or point mutations in the CARD2-Hel2i interface decreases the selectivity from ≈3000 to 150 and 750, respectively. We propose that the CARD2-Hel2i interface is a 'gate' that prevents cellular RNAs from generating productive complexes that can signal. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  5. Testing for voter rigging in small polling stations

    PubMed Central

    Jimenez, Raúl; Hidalgo, Manuel; Klimek, Peter

    2017-01-01

    Nowadays, a large number of countries combine formal democratic institutions with authoritarian practices. Although in these countries the ruling elites may receive considerable voter support, they often use several manipulation tools to control election outcomes. A common practice of these regimes is the coercion and mobilization of large numbers of voters. This electoral irregularity is known as voter rigging, distinguishing it from vote rigging, which involves ballot stuffing or stealing. We develop a statistical test to quantify the extent to which the results of a particular election display traces of voter rigging. Our key hypothesis is that small polling stations are more susceptible to voter rigging because it is easier to identify opposing individuals, there are fewer eyewitnesses, and interested parties might reasonably expect fewer visits from election observers. We devise a general statistical method for testing whether voting behavior in small polling stations is significantly different from the behavior in their neighbor stations in a way that is consistent with the widespread occurrence of voter rigging. On the basis of a comparative analysis, the method enables third parties to conclude that an explanation other than simple variability is needed to explain geographic heterogeneities in vote preferences. We analyze 21 elections in 10 countries and find significant statistical anomalies compatible with voter rigging in Russia from 2007 to 2011, in Venezuela from 2006 to 2013, and in Uganda in 2011. Particularly disturbing is the case of Venezuela, where the smallest polling stations were decisive to the outcome of the 2013 presidential elections. PMID:28695193

  6. Type I IFN triggers RIG-I/TLR3/NLRP3-dependent inflammasome activation in influenza A virus infected cells.

    PubMed

    Pothlichet, Julien; Meunier, Isabelle; Davis, Beckley K; Ting, Jenny P-Y; Skamene, Emil; von Messling, Veronika; Vidal, Silvia M

    2013-01-01

    Influenza A virus (IAV) triggers a contagious and potentially lethal respiratory disease. A protective IL-1β response is mediated by innate receptors in macrophages and lung epithelial cells. NLRP3 is crucial in macrophages; however, which sensors elicit IL-1β secretion in lung epithelial cells remains undetermined. Here, we describe for the first time the relative roles of the host innate receptors RIG-I (DDX58), TLR3, and NLRP3 in the IL-1β response to IAV in primary lung epithelial cells. To activate IL-1β secretion, these cells employ partially redundant recognition mechanisms that differ from those described in macrophages. RIG-I had the strongest effect through a MAVS/TRIM25/Riplet-dependent type I IFN signaling pathway upstream of TLR3 and NLRP3. Notably, RIG-I also activated the inflammasome through interaction with caspase 1 and ASC in primary lung epithelial cells. Thus, NS1, an influenza virulence factor that inhibits the RIG-I/type I IFN pathway, strongly modulated the IL-1β response in lung epithelial cells and in ferrets. The NS1 protein derived from a highly pathogenic strain resulted in increased interaction with RIG-I and inhibited type I IFN and IL-1β responses compared to the least pathogenic virus strains. These findings demonstrate that in IAV-infected lung epithelial cells RIG-I activates the inflammasome both directly and through a type I IFN positive feedback loop.

  7. Testing for voter rigging in small polling stations.

    PubMed

    Jimenez, Raúl; Hidalgo, Manuel; Klimek, Peter

    2017-06-01

    Nowadays, a large number of countries combine formal democratic institutions with authoritarian practices. Although in these countries the ruling elites may receive considerable voter support, they often use several manipulation tools to control election outcomes. A common practice of these regimes is the coercion and mobilization of large numbers of voters. This electoral irregularity is known as voter rigging, distinguishing it from vote rigging, which involves ballot stuffing or stealing. We develop a statistical test to quantify the extent to which the results of a particular election display traces of voter rigging. Our key hypothesis is that small polling stations are more susceptible to voter rigging because it is easier to identify opposing individuals, there are fewer eyewitnesses, and interested parties might reasonably expect fewer visits from election observers. We devise a general statistical method for testing whether voting behavior in small polling stations is significantly different from the behavior in their neighbor stations in a way that is consistent with the widespread occurrence of voter rigging. On the basis of a comparative analysis, the method enables third parties to conclude that an explanation other than simple variability is needed to explain geographic heterogeneities in vote preferences. We analyze 21 elections in 10 countries and find significant statistical anomalies compatible with voter rigging in Russia from 2007 to 2011, in Venezuela from 2006 to 2013, and in Uganda in 2011. Particularly disturbing is the case of Venezuela, where the smallest polling stations were decisive to the outcome of the 2013 presidential elections.

  8. GUCY2C Signaling Opposes the Acute Radiation-Induced GI Syndrome.

    PubMed

    Li, Peng; Wuthrick, Evan; Rappaport, Jeff A; Kraft, Crystal; Lin, Jieru E; Marszalowicz, Glen; Snook, Adam E; Zhan, Tingting; Hyslop, Terry M; Waldman, Scott A

    2017-09-15

    High doses of ionizing radiation induce acute damage to epithelial cells of the gastrointestinal (GI) tract, mediating toxicities restricting the therapeutic efficacy of radiation in cancer and morbidity and mortality in nuclear disasters. No approved prophylaxis or therapy exists for these toxicities, in part reflecting an incomplete understanding of mechanisms contributing to the acute radiation-induced GI syndrome (RIGS). Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. Here, we reveal a role for the GUCY2C paracrine axis in compensatory mechanisms opposing RIGS. Eliminating GUCY2C signaling exacerbated RIGS, amplifying radiation-induced mortality, weight loss, mucosal bleeding, debilitation, and intestinal dysfunction. Durable expression of GUCY2C, guanylin, and uroguanylin mRNA and protein by intestinal epithelial cells was preserved following lethal irradiation inducing RIGS. Oral delivery of the heat-stable enterotoxin (ST), an exogenous GUCY2C ligand, opposed RIGS, a process requiring p53 activation mediated by dissociation from MDM2. In turn, p53 activation prevented cell death by selectively limiting mitotic catastrophe, but not apoptosis. These studies reveal a role for the GUCY2C paracrine hormone axis as a novel compensatory mechanism opposing RIGS, and they highlight the potential of oral GUCY2C agonists (Linzess; Trulance) to prevent and treat RIGS in cancer therapy and nuclear disasters. Cancer Res; 77(18); 5095-106. ©2017 AACR . ©2017 American Association for Cancer Research.

  9. Hydraulic drill string breakdown and bleed off unit

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zeringue, F.J. Jr.

    1987-02-17

    An apparatus is described for use within an oil well rig for decoupling a tubing string into pipe segments comprising, in combination: rotary tong means for applying an unthreading torque to a first, upper pipe segment within the tubing string; torque resisting means for securing a second, lower pipe segment within the tubing string against the unthreading torque; containing means, intermediate the rotary tong means and the torque resisting means, enclosing a threaded joint of the tubing string, adapted for containing pressurized gases, liquids, and particulates, released from the threaded joint upon the decoupling; fluid communicating means for allowing fluidmore » communication between the containing means and a receiving point adapted for receiving the pressurized gases, liquids, and particulates; means for moving the rotary tong means, the torque resisting means and the containing means between a closed, engaging position with the tubing string and an open position; and means for horizontally moving the rotary tong means, the torque resisting means and the containing means between a position adjacent the tubing string and a position away from the tubing string.« less

  10. δ(13)C and δ(15)N in deep-living fishes and shrimps after the Deepwater Horizon oil spill, Gulf of Mexico.

    PubMed

    Quintana-Rizzo, Ester; Torres, Joseph J; Ross, Steve W; Romero, Isabel; Watson, Kathleen; Goddard, Ethan; Hollander, David

    2015-05-15

    The blowout of the Deepwater Horizon (DWH) drill-rig produced a surface oil layer, dispersed micro-droplets throughout the water column, and sub-surface plumes. We measured stable carbon and nitrogen isotopes in mesopelagic fishes and shrimps in the vicinity of DWH collected prior to, six weeks after, and one year after the oil spill (2007, 2010 and 2011). In 2010, the year of the oil spill, a small but significant depletion of δ(13)C was found in two mesopelagic fishes (Gonostoma elongatum and Chauliodus sloani) and one shrimp (Systellaspis debilis); a significant δ(15)N enrichment was identified in the same shrimp and in three fish species (G. elongatum, Ceratoscopelus warmingii, and Lepidophanes guentheri). The δ(15)N change did not suggest a change of trophic level, but did indicate a change in diet. The data suggest that carbon from the Deepwater Horizon oil spill was incorporated into the mesopelagic food web of the Gulf of Mexico. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. USGS/EPA collection protocol for bacterial pathogens in soil

    USGS Publications Warehouse

    Griffin, Dale W.; Shaefer, F.L.; Charlena Bowling,; Dino Mattorano,; Tonya Nichols,; Erin Silvestri,

    2014-01-01

    This Sample Collection Procedure (SCP) describes the activities and considerations for the collection of bacterial pathogens from representative surface soil samples (0-5 cm). This sampling depth can be reached without the use of a drill rig, direct-push technology, or other mechanized equipment. This procedure can be used in most soil types but is limited to sampling at or near the ground surface. This protocol has components for two different types of sampling applications: (1) typical sampling, when there is no suspicion of contamination (e.g., surveillance or background studies); and (2) in response to known or suspected accidental contamination (e.g., the presence of animal carcasses). This protocol does not cover sampling in response to a suspected bioterrorist or intentional release event. Surface material is removed to the required depth (0-5 cm) and clean trowel or 50 ml sample tube is used to collect the sample. Sample containers are sealed, bagged, and shipped to the laboratory for analysis. Associated documentation, including a Field Data Log and Chain-of-Custody are also included in this document.

  12. The Effect of Applied Tensile Stress on Localized Corrosion in Sensitized AA5083

    DTIC Science & Technology

    2015-09-01

    of stainless steel 4-point bending rig used to apply elastic stress to aluminum plate samples. (Bottom) Stress- strain data based on displacement and...ASTM-G39, from [25]. ..........................20 Figure 13. Photograph of stainless steel 4-point bending rig used to apply elastic stress to...aluminum plate samples, from [8]. ....................................................20 Figure 14. Photograph of stainless steel 4-point bending rig

  13. A numerical model of a HIL scaled roller rig for simulation of wheel-rail degraded adhesion condition

    NASA Astrophysics Data System (ADS)

    Conti, Roberto; Meli, Enrico; Pugi, Luca; Malvezzi, Monica; Bartolini, Fabio; Allotta, Benedetto; Rindi, Andrea; Toni, Paolo

    2012-05-01

    Scaled roller rigs used for railway applications play a fundamental role in the development of new technologies and new devices, combining the hardware in the loop (HIL) benefits with the reduction of the economic investments. The main problem of the scaled roller rig with respect to the full scale ones is the improved complexity due to the scaling factors. For this reason, before building the test rig, the development of a software model of the HIL system can be useful to analyse the system behaviour in different operative conditions. One has to consider the multi-body behaviour of the scaled roller rig, the controller and the model of the virtual vehicle, whose dynamics has to be reproduced on the rig. The main purpose of this work is the development of a complete model that satisfies the previous requirements and in particular the performance analysis of the controller and of the dynamical behaviour of the scaled roller rig when some disturbances are simulated with low adhesion conditions. Since the scaled roller rig will be used to simulate degraded adhesion conditions, accurate and realistic wheel-roller contact model also has to be included in the model. The contact model consists of two parts: the contact point detection and the adhesion model. The first part is based on a numerical method described in some previous studies for the wheel-rail case and modified to simulate the three-dimensional contact between revolute surfaces (wheel-roller). The second part consists in the evaluation of the contact forces by means of the Hertz theory for the normal problem and the Kalker theory for the tangential problem. Some numerical tests were performed, in particular low adhesion conditions were simulated, and bogie hunting and dynamical imbalance of the wheelsets were introduced. The tests were devoted to verify the robustness of control system with respect to some of the more frequent disturbances that may influence the roller rig dynamics. In particular we verified that the wheelset imbalance could significantly influence system performance, and to reduce the effect of this disturbance a multistate filter was designed.

  14. Overcoming codon bias: a method for high-level overexpression of Plasmodium and other AT-rich parasite genes in Escherichia coli.

    PubMed

    Baca, A M; Hol, W G

    2000-02-01

    Parasite genes often use codons which are rarely used in the highly expressed genes of Escherichia coli, possibly resulting in translational stalling and lower yields of recombinant protein. We have constructed the "RIG" plasmid to overcome the potential codon-bias problem seen in Plasmodium genes. RIG contains the genes that encode three tRNAs (Arg, Ile, Gly), which recognise rare codons found in parasite genes. When co-transformed into E. coli along with expression plasmids containing parasite genes, RIG can greatly increase levels of overexpressed protein. Codon frequency analysis suggests that RIG may be applied to a variety of protozoan and helminth genes.

  15. Energy efficient engine: High pressure turbine uncooled rig technology report

    NASA Technical Reports Server (NTRS)

    Gardner, W. B.

    1979-01-01

    Results obtained from testing five performance builds (three vane cascades and two rotating rigs of the Energy Efficient Engine uncooled rig have established the uncooled aerodynamic efficiency of the high-pressure turbine at 91.1 percent. This efficiency level was attained by increasing the rim speed and annulus area (AN(2)), and by increasing the turbine reaction level. The increase in AN(2) resulted in a performance improvement of 1.15 percent. At the design point pressure ratio, the increased reaction level rig demonstrated an efficiency of 91.1 percent. The results of this program have verified the aerodynamic design assumptions established for the Energy Efficient Engine high-pressure turbine component.

  16. Stratified charge rotary aircraft engine technology enablement program

    NASA Technical Reports Server (NTRS)

    Badgley, P. R.; Irion, C. E.; Myers, D. M.

    1985-01-01

    The multifuel stratified charge rotary engine is discussed. A single rotor, 0.7L/40 cu in displacement, research rig engine was tested. The research rig engine was designed for operation at high speeds and pressures, combustion chamber peak pressure providing margin for speed and load excursions above the design requirement for a high is advanced aircraft engine. It is indicated that the single rotor research rig engine is capable of meeting the established design requirements of 120 kW, 8,000 RPM, 1,379 KPA BMEP. The research rig engine, when fully developed, will be a valuable tool for investigating, advanced and highly advanced technology components, and provide an understanding of the stratified charge rotary engine combustion process.

  17. Vibrio vulnificus quorum-sensing molecule cyclo(Phe-Pro) inhibits RIG-I-mediated antiviral innate immunity.

    PubMed

    Lee, Wooseong; Lee, Seung-Hoon; Kim, Minwoo; Moon, Jae-Su; Kim, Geon-Woo; Jung, Hae-Gwang; Kim, In Hwang; Oh, Ji Eun; Jung, Hi Eun; Lee, Heung Kyu; Ku, Keun Bon; Ahn, Dae-Gyun; Kim, Seong-Jun; Kim, Kun-Soo; Oh, Jong-Won

    2018-04-23

    The recognition of pathogen-derived ligands by pattern recognition receptors activates the innate immune response, but the potential interaction of quorum-sensing (QS) signaling molecules with host anti-viral defenses remains largely unknown. Here we show that the Vibrio vulnificus QS molecule cyclo(Phe-Pro) (cFP) inhibits interferon (IFN)-β production by interfering with retinoic-acid-inducible gene-I (RIG-I) activation. Binding of cFP to the RIG-I 2CARD domain induces a conformational change in RIG-I, preventing the TRIM25-mediated ubiquitination to abrogate IFN production. cFP enhances susceptibility to hepatitis C virus (HCV), as well as Sendai and influenza viruses, each known to be sensed by RIG-I but did not affect the melanoma-differentiation-associated gene 5 (MDA5)-recognition of norovirus. Our results reveal an inter-kingdom network between bacteria, viruses and host that dysregulates host innate responses via a microbial quorum-sensing molecule modulating the response to viral infection.

  18. Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by RIG-I

    PubMed Central

    Gack, Michaela Ulrike; Albrecht, Randy Allen; Urano, Tomohiko; Inn, Kyung-Soo; Huang, I-Chueh; Carnero, Elena; Farzan, Michael; Inoue, Satoshi; Jung, Jae Ung; García-Sastre, Adolfo

    2009-01-01

    SUMMARY TRIM25 mediates Lys 63-linked ubiquitination of the N-terminal CARDs of the viral RNA sensor RIG-I, leading to type I interferon (IFN) production. Here, we report that the influenza A virus non-structural protein 1 (NS1) specifically inhibits TRIM25-mediated RIG-I CARD ubiquitination, thereby suppressing RIG-I signal transduction. A novel domain in NS1 comprising E96/E97 residues mediates its interaction with the coiled-coil domain of TRIM25, thus blocking TRIM25 multimerization and RIG-I CARD ubiquitination. Furthermore, a recombinant influenza A virus expressing an E96A/E97A NS1 mutant is defective in blocking TRIM25-mediated anti-viral IFN response and loses virulence in mice. Our findings reveal a novel mechanism of influenza virus to inhibit host IFN response and also emphasize the vital role of TRIM25 in modulating viral infections. PMID:19454348

  19. Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I.

    PubMed

    Gack, Michaela Ulrike; Albrecht, Randy Allen; Urano, Tomohiko; Inn, Kyung-Soo; Huang, I-Chueh; Carnero, Elena; Farzan, Michael; Inoue, Satoshi; Jung, Jae Ung; García-Sastre, Adolfo

    2009-05-08

    The ubiquitin ligase TRIM25 mediates Lysine 63-linked ubiquitination of the N-terminal CARD domains of the viral RNA sensor RIG-I to facilitate type I interferon (IFN) production and antiviral immunity. Here, we report that the influenza A virus nonstructural protein 1 (NS1) specifically inhibits TRIM25-mediated RIG-I CARD ubiquitination, thereby suppressing RIG-I signal transduction. A novel domain in NS1 comprising E96/E97 residues mediates its interaction with the coiled-coil domain of TRIM25, thus blocking TRIM25 multimerization and RIG-I CARD domain ubiquitination. Furthermore, a recombinant influenza A virus expressing an E96A/E97A NS1 mutant is defective in blocking TRIM25-mediated antiviral IFN response and loses virulence in mice. Our findings reveal a mechanism by which influenza virus inhibits host IFN response and also emphasize the vital role of TRIM25 in modulating antiviral defenses.

  20. ECSIT bridges RIG-I-like receptors to VISA in signaling events of innate antiviral responses.

    PubMed

    Lei, Cao-Qi; Zhang, Yu; Li, Mi; Jiang, Li-Qun; Zhong, Bo; Kim, Yong Ho; Shu, Hong-Bing

    2015-01-01

    Upon binding to RNA structures from invading viruses, RIG-I and MDA5 are recruited to mitochondria to interact with VISA and initiate antiviral type I interferon (IFN) responses. How this process is mediated is less understood. In this report, we demonstrate that ECSIT is an essential scaffolding protein that mediates the association of VISA and RIG-I or MDA5. Overexpression of ECSIT potentiated virus-triggered activation of IFN-regulatory factor 3 (IRF3) and expression of IFNB1, whereas knockdown of ECSIT impaired viral infection-induced activation of IRF3 and expression of IFNB1 as well as cellular antiviral responses. Mechanistically, ECSIT was associated with VISA on mitochondria and important for bridging RIG-I and MDA5 to VISA. Our findings suggest that ECSIT mediates virus-triggered type I IFN induction by bridging RIG-I and MDA5 to the VISA complex, and provide new insights into the molecular events of innate antiviral immune responses. © 2014 S. Karger AG, Basel.

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