Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates.
Meyer, Christine; Haberthur, Kristen; Asquith, Mark; Messaoudi, Ilhem
2015-01-01
Flow cytometry is an invaluable technique that can be used to phenotypically and functionally characterize immune cell populations ex vivo. This technology has greatly advanced our ability to gain critical insight into age-related changes in immune function, commonly known as immune senescence. Rodents have been traditionally used to investigate the molecular mechanisms of immune senescence because they offer the distinct advantages of an extensive set of reagents, the presence of genetically modified strains, and a short lifespan that allows for longevity studies of short duration. More recently, nonhuman primates (NHPs), and specifically rhesus macaques, have emerged as a leading translational model to study various aspects of human aging. In contrast to rodents, they share significant genetic homology as well as physiological and behavioral characteristics with humans. Furthermore, rhesus macaques are a long-lived outbred species, which makes them an ideal translational model. Therefore, NHPs offer a unique opportunity to carry out mechanistic studies under controlled laboratory conditions (e.g., photoperiod, temperature, diet, and medications) in a species that closely mimics human biology. Moreover similar techniques (e.g., activity recording and MRI) can be used to measure physiological parameters in NHPs, making direct comparisons between NHP and human data sets possible. In addition, the outbred genetics of NHPs enables rigorous validation of research findings that goes beyond proof of principle. Finally, self-selection bias that is often unavoidable in human clinical trials can be completely eliminated with NHP studies. Here we describe flow cytometry-based methods to phenotypically and functionally characterize innate immune cells as well as T and B lymphocyte subsets from isolated peripheral blood mononuclear cells (PBMC) in rhesus macaques. PMID:26420709
Dhoble, Abhishek S; Bekal, Sadia; Dolatowski, William; Yanz, Connor; Lambert, Kris N; Bhalerao, Kaustubh D
2016-11-01
A novel multidimensional flow cytometry based method has been demonstrated to monitor and rapidly characterize the dynamics of the complex anaerobic microbiome associated with perturbations in external environmental factors. While community fingerprinting provides an estimate of the meta genomic structure, flow cytometry provides a fingerprint of the community morphology including its autofluorescence spectrum in a high-throughput manner. Using anaerobic microbial consortia perturbed with the controlled addition of various carbon sources, it is possible to quantitatively discriminate between divergent microbiome analogous to community fingerprinting techniques using automated ribosomal intergenic spacer analysis (ARISA). The utility of flow cytometry based method has also been demonstrated in a fully functional industry scale anaerobic digester to distinguish between microbiome composition caused by varying hydraulic retention time (HRT). This approach exploits the rich multidimensional information from flow cytometry for rapid characterization of the dynamics of microbial communities. PMID:27614579
Xue, Yong; Wilkes, Jon G; Moskal, Ted J; Williams, Anna J; Cooper, Willie M; Nayak, Rajesh; Rafii, Fatemeh; Buzatu, Dan A
2016-01-01
Standard methods to detect Escherichia coli contamination in food use the polymerase chain reaction (PCR) and agar culture plates. These methods require multiple incubation steps and take a long time to results. An improved rapid flow-cytometry based detection method was developed, using a fluorescence-labeled oligonucleotide probe specifically binding a16S rRNA sequence. The method positively detected 51 E. coli isolates as well as 4 Shigella species. All 27 non-E. coli strains tested gave negative results. Comparison of the new genetic assay with a total plate count (TPC) assay and agar plate counting indicated similar sensitivity, agreement between cytometry cell and colony counts. This method can detect a small number of E.coli cells in the presence of large numbers of other bacteria. This method can be used for rapid, economical, and stable detection of E. coli and Shigella contamination in the food industry and other contexts.
Xue, Yong; Wilkes, Jon G; Moskal, Ted J; Williams, Anna J; Cooper, Willie M; Nayak, Rajesh; Rafii, Fatemeh; Buzatu, Dan A
2016-01-01
Standard methods to detect Escherichia coli contamination in food use the polymerase chain reaction (PCR) and agar culture plates. These methods require multiple incubation steps and take a long time to results. An improved rapid flow-cytometry based detection method was developed, using a fluorescence-labeled oligonucleotide probe specifically binding a16S rRNA sequence. The method positively detected 51 E. coli isolates as well as 4 Shigella species. All 27 non-E. coli strains tested gave negative results. Comparison of the new genetic assay with a total plate count (TPC) assay and agar plate counting indicated similar sensitivity, agreement between cytometry cell and colony counts. This method can detect a small number of E.coli cells in the presence of large numbers of other bacteria. This method can be used for rapid, economical, and stable detection of E. coli and Shigella contamination in the food industry and other contexts. PMID:26913737
Xue, Yong; Wilkes, Jon G.; Moskal, Ted J.; Williams, Anna J.; Cooper, Willie M.; Nayak, Rajesh; Rafii, Fatemeh; Buzatu, Dan A.
2016-01-01
Standard methods to detect Escherichia coli contamination in food use the polymerase chain reaction (PCR) and agar culture plates. These methods require multiple incubation steps and take a long time to results. An improved rapid flow-cytometry based detection method was developed, using a fluorescence-labeled oligonucleotide probe specifically binding a16S rRNA sequence. The method positively detected 51 E. coli isolates as well as 4 Shigella species. All 27 non-E. coli strains tested gave negative results. Comparison of the new genetic assay with a total plate count (TPC) assay and agar plate counting indicated similar sensitivity, agreement between cytometry cell and colony counts. This method can detect a small number of E.coli cells in the presence of large numbers of other bacteria. This method can be used for rapid, economical, and stable detection of E. coli and Shigella contamination in the food industry and other contexts. PMID:26913737
Forment, Josep V.; Jackson, Stephen P.
2016-01-01
Protein accumulation on chromatin has traditionally been studied using immunofluorescence microscopy or biochemical cellular fractionation followed by western immunoblot analysis. As a way to improve the reproducibility of this kind of analysis, make it easier to quantify and allow a stream-lined application in high-throughput screens, we recently combined a classical immunofluorescence microscopy detection technique with flow cytometry1. In addition to the features described above, and by combining it with detection of both DNA content and DNA replication, this method allows unequivocal and direct assignment of cell-cycle distribution of protein association to chromatin without the need for cell culture synchronization. Furthermore, it is relatively quick (no more than a working day from sample collection to quantification), requires less starting material compared to standard biochemical fractionation methods and overcomes the need for flat, adherent cell types that are required for immunofluorescence microscopy. PMID:26226461
Fraga, María; Vilariño, Natalia; Louzao, M Carmen; Fernández, Diego A; Poli, Mark; Botana, Luis M
2016-01-15
Palytoxin (PLTX) is a complex marine toxin produced by zoanthids (i.e. Palythoa), dinoflagellates (Ostreopsis) and cyanobacteria (Trichodesmium). PLTX outbreaks are usually associated with Indo-Pacific waters, however their recent repeated occurrence in Mediterranean-European Atlantic coasts demonstrate their current worldwide distribution. Human sickness and fatalities have been associated with toxic algal blooms and ingestion of seafood contaminated with PLTX-like molecules. These toxins represent a serious threat to human health. There is an immediate need to develop easy-to-use, rapid detection methods due to the lack of validated protocols for their detection and quantification. We have developed an immuno-detection method for PLTX-like molecules based on the use of microspheres coupled to flow-cytometry detection (Luminex 200™). The assay consisted of the competition between free PLTX-like compounds in solution and PLTX immobilized on the surface of microspheres for binding to a specific monoclonal anti-PLTX antibody. This method displays an IC50 of 1.83 ± 0.21 nM and a dynamic range of 0.47-6.54 nM for PLTX. An easy-to-perform extraction protocol, based on a mixture of methanol and acetate buffer, was applied to spiked mussel samples providing a recovery rate of 104 ± 8% and a range of detection from 374 ± 81 to 4430 ± 150 μg kg(-1) when assayed with this method. Extracts of Ostreopsis cf. siamensis and Palythoa tuberculosa were tested and yielded positive results for PLTX-like molecules. However, the data obtained for the coral sample suggested that this antibody did not detect 42-OH-PLTX efficiently. The same samples were further analyzed using a neuroblastoma cytotoxicity assay and UPLC-IT-TOF spectrometry, which also pointed to the presence of PLTX-like compounds. Therefore, this single detection method for PLTX provides a semi-quantitative tool useful for the screening of PLTX-like molecules in different matrixes. PMID:26709295
Gray, Sean; Weigel, Kris M.; Miller, Keith D.; Ndung'u, Joseph; Buscher, Philippe; Tran, Thao N.; Baird, Cheryl L.; Cangelosi, Gerard A.
2010-04-01
Novel methods are reported for evaluating and utilizing single chain fragment variable (scFv) antibodies derived from yeast-display libraries. Yeast-display was used to select scFv specific to invariant surface glycoproteins (ISG) of Trypanosoma brucei. A limiting step in the isolation of scFv from nonimmune libraries is the conversion of highly active yeast-displayed scFv into soluble antibodies that can be used in standard immunoassays. Challenges include limited solubility or activity following secretion and purification of scFv. For this reason, few scFv derived from yeast-display platforms have moved into development and implementation as diagnostic reagents. To address this problem, assays were developed that employ both yeastdisplayed and secreted scFv as analytical reagents. The first is a competitive inhibition flow cytometry (CIFC) assay that detects secreted scFv by virtue of its ability to competitively inhibit the binding of biotinylated antigen to yeast-displayed scFv. The second is an epitope binning assay that uses secreted scFv toidentify additional yeast-displayed scFv that bind nonoverlapping or noncompeting epitopes on an antigen. The epitope binning assay was used not only to identify sandwich assay pairs with yeast-displayed scFv, but also to identify active soluble scFv present in low concentration in a crude expression extract. Finally, a CIFC assay was developed that bypasses entirely the need for soluble scFv expression, by using yeast displayed scFv to detect unlabeled antigen in samples. These methods will facilitate the continued development and practical implementation of scFv derived from yeast-display libraries.
Gray, Sean A.; Weigel, Kris M.; Miller, Keith D.; Ndung'u, Joseph; Büscher, Philippe; Tran, Thao; Baird, Cheryl; Cangelosi, Gerard A.
2010-01-01
Novel methods are reported for evaluating and utilizing single chain fragment variable (scFv) antibodies derived from yeast-display libraries. Yeast-display was used to select scFv specific to invariant surface glycoproteins (ISG) of Trypanosoma brucei. Alimiting step in the isolation of scFv from non-immune libraries is the conversion of highly active yeast-displayed scFv into soluble antibodies that can be used in standard immunoassays. Challenges include limited solubility or activity following secretion and purification of scFv. For this reason, few scFv derived from yeast-display platforms have moved into development and implementation as diagnostic reagents. To address this problem, assays were developed that employ both yeast-displayed and -secreted scFv as analytical reagents. The first is a competitive inhibition flow cytometry (CIFC) assay that detects secreted scFv by virtue of their ability to competitively inhibit the binding of biotinylated antigen to yeast-displayed scFv. The second is an epitope binning assay that uses secreted scFv to identify additional yeast-displayed scFv that bind non-overlapping or non-competing epitopes on an antigen. The epitope binning assay was used not only to identify sandwich assay pairs with yeast-displayed scFv, but also to identify active soluble scFv present in low concentration in a crude expression extract. Finally, a CIFC assay was developed that bypasses entirely the need for soluble scFv expression, by using yeast-displayed scFv to detect unlabeled antigen in samples. These methods will facilitate the continued development and practical implementation of scFv derived from yeast-display libraries. PMID:19953671
Flow-cytometry-based DNA hybidization and polymorphism analysis
NASA Astrophysics Data System (ADS)
Cai, Hong; Kommander, Kristina; White, P. S.; Nolan, John P.
1998-05-01
Functional analysis of the human genome, including the quantification of differential gene expression and the identification of polymorphic sites and disease genes, is an important element of the Human Genome Project. Current methods of analysis are mainly gel-based assays that are not well- suited to rapid genome-scale analyses. To analyze DNA sequence on a large scale, robust and high throughput assays are needed. We are developing a suite of microsphere-based approaches employing fluorescence detection to screen and analyze genomic sequence. Our approaches include competitive DNA hybridization to measure DNA or RNA targets in unknown samples, and oligo ligation or extension assays to analyze single-nucleotide polymorphisms. Apart from the advantages of sensitivity, simplicity, and low sample consumption, these flow cytometric approaches have the potential for high throughput multiplexed analysis using multicolored microspheres and automated sample handling.
Detecting endotoxin with a flow cytometry-based magnetic aptasensor.
Zuo, Ming-Yan; Chen, Li-Juan; Jiang, Hao; Tan, Lin; Luo, Zhao-Feng; Wang, Yan-Mei
2014-12-01
Endotoxin, which is also known as lipopolysaccharide (LPS), is a marker for intruding gram-negative pathogens. It is essential to detect endotoxin quickly and sensitively in a complex milieu. A new flow cytometry (FCM)-based magnetic aptasensor assay that employs two endotoxin-binding aptamers and magnetic beads has been developed to detect endotoxin. The endotoxin-conjugated sandwich complex on magnetic beads was observed by scanning confocal laser microscopy. The resulting magnetic aptasensor rapidly detected (<1 min) endotoxin within a broad dynamic detection range of 10(-8) to 10(0)mg/ml in the presence of bovine serum albumin (BSA), RNA, sucrose, and glucose, which are most likely to coexist with endotoxin in the majority of biological liquids. Only 2 μl of magnetic aptasensor was required to quantify the endotoxin solution. Furthermore, the magnetic aptasensor could be regenerated seven times and still presented an outstanding response to the endotoxin solution. Therefore, the magnetic aptasensor exhibited high sensitivity, selectivity, and reproducibility, thereby serving as a powerful tool for the quality control and high-throughput detection of endotoxin in the food and pharmaceutical industries.
A flow cytometry-based dopamine transporter binding assay using antagonist-conjugated quantum dots
Kovtun, Oleg; Ross, Emily; Tomlinson, Ian; Rosenthal, Sandra
2012-01-01
Here we present the development and validation of a flow cytometry-based dopamine transporter (DAT) binding assay that uses antagonist-conjugated quantum dots (QDs).We anticipate that our QD-based assay is of immediate value to the high throughput screening of novel DAT modulators.
Cedeño-Arias, Mercedes; Sánchez-Ramírez, Javier; Blanco-Santana, Rancés; Rengifo-Calzado, Enrique
2011-01-01
An ideal test used to characterize a product must be appropriate for the measurement of product quality, manufacturing consistency, product stability, and comparability studies. Flow cytometry has been successfully applied to the examination of antibodies and receptors on membrane surfaces; however, to date, the analytical validation of cytometry based assays is limited. Here we report on the validation of a flow cytometry-based assay used in the evaluation of nimotuzumab binding to cells over-expressing EGFR on cell surface. The assay was validated by examining, assay robustness, specificity, repeatability and intermediate precision. The assay was highly specific, robust for all studied factors except for cell fixation with 1% paraformaldehyde and met criteria for precision with RSD < 2%. In addition the assay has stability-indicating properties evidenced by the ability to detect changes in mAb degraded samples. Most importantly, the assay demonstrated to be useful for its intended use. PMID:21886904
In vitro flow cytometry-based screening platform for cellulase engineering
Körfer, Georgette; Pitzler, Christian; Vojcic, Ljubica; Martinez, Ronny; Schwaneberg, Ulrich
2016-01-01
Ultrahigh throughput screening (uHTS) plays an essential role in directed evolution for tailoring biocatalysts for industrial applications. Flow cytometry-based uHTS provides an efficient coverage of the generated protein sequence space by analysis of up to 107 events per hour. Cell-free enzyme production overcomes the challenge of diversity loss during the transformation of mutant libraries into expression hosts, enables directed evolution of toxic enzymes, and holds the promise to efficiently design enzymes of human or animal origin. The developed uHTS cell-free compartmentalization platform (InVitroFlow) is the first report in which a flow cytometry-based screened system has been combined with compartmentalized cell-free expression for directed cellulase enzyme evolution. InVitroFlow was validated by screening of a random cellulase mutant library employing a novel screening system (based on the substrate fluorescein-di-β-D-cellobioside), and yielded significantly improved cellulase variants (e.g. CelA2-H288F-M1 (N273D/H288F/N468S) with 13.3-fold increased specific activity (220.60 U/mg) compared to CelA2 wildtype: 16.57 U/mg). PMID:27184298
In vitro flow cytometry-based screening platform for cellulase engineering.
Körfer, Georgette; Pitzler, Christian; Vojcic, Ljubica; Martinez, Ronny; Schwaneberg, Ulrich
2016-01-01
Ultrahigh throughput screening (uHTS) plays an essential role in directed evolution for tailoring biocatalysts for industrial applications. Flow cytometry-based uHTS provides an efficient coverage of the generated protein sequence space by analysis of up to 10(7) events per hour. Cell-free enzyme production overcomes the challenge of diversity loss during the transformation of mutant libraries into expression hosts, enables directed evolution of toxic enzymes, and holds the promise to efficiently design enzymes of human or animal origin. The developed uHTS cell-free compartmentalization platform (InVitroFlow) is the first report in which a flow cytometry-based screened system has been combined with compartmentalized cell-free expression for directed cellulase enzyme evolution. InVitroFlow was validated by screening of a random cellulase mutant library employing a novel screening system (based on the substrate fluorescein-di-β-D-cellobioside), and yielded significantly improved cellulase variants (e.g. CelA2-H288F-M1 (N273D/H288F/N468S) with 13.3-fold increased specific activity (220.60 U/mg) compared to CelA2 wildtype: 16.57 U/mg). PMID:27184298
Measuring antibody neutralization of dengue virus (DENV) using a flow cytometry-based technique.
de Alwis, Ruklanthi; de Silva, Aravinda M
2014-01-01
Dengue virus (DENV) is an emerging virus that threatens over two-third of the world's population. The specific diagnosis of dengue infection by serology is based on assays that detect DENV-specific antibodies including neutralizing antibodies (Abs). Neutralizing Abs are an important, if not the main, mechanism of protection from natural dengue virus (DENV) infection as well. The current gold-standard assay for measuring neutralizing Ab responses against DENV is the plaque reduction neutralization assay (PRNT). However, this assay is slow and laborious and utilizes physiologically irrelevant cell lines. Here, we describe a relatively high-throughput, flow cytometry-based neutralization assay for DENV that has been optimized for use with a human monocytic suspension cell line, U937 + DC-SIGN, or the more commonly used adherent monkey kidney cells, Vero-81. PMID:24696329
Böhme, Steffi; Stärk, Hans-Joachim; Meißner, Tobias; Springer, Armin; Reemtsma, Thorsten; Kühnel, Dana; Busch, Wibke
2014-01-01
In order to quantify and compare the uptake of aluminum oxide nanoparticles of three different sizes into two human cell lines (skin keratinocytes (HaCaT) and lung epithelial cells (A549)), three analytical methods were applied: digestion followed by nebulization inductively coupled plasma mass spectrometry (neb-ICP-MS), direct laser ablation ICP-MS (LA-ICP-MS), and flow cytometry. Light and electron microscopy revealed an accumulation and agglomeration of all particle types within the cell cytoplasm, whereas no particles were detected in the cell nuclei. The internalized Al2O3 particles exerted no toxicity in the two cell lines after 24 h of exposure. The smallest particles with a primary particle size (x BET) of 14 nm (Alu1) showed the lowest sedimentation velocity within the cell culture media, but were calculated to have settled completely after 20 h. Alu2 (x BET = 111 nm) and Alu3 (x BET = 750 nm) were calculated to reach the cell surface after 7 h and 3 min, respectively. The internal concentrations determined with the different methods lay in a comparable range of 2-8 µg Al2O3/cm(2) cell layer, indicating the suitability of all methods to quantify the nanoparticle uptake. Nevertheless, particle size limitations of analytical methods using optical devices were demonstrated for LA-ICP-MS and flow cytometry. Furthermore, the consideration and comparison of particle properties as parameters for particle internalization revealed the particle size and the exposure concentration as determining factors for particle uptake. PMID:25285033
Urbani, Francesca; Proietti, Enrico
2013-01-01
The development of immune monitoring assays is essential to determine the immune responses against tumor-specific antigens (TSAs) and tumor-associated antigens (TAAs) and their possible correlation with clinical outcome in cancer patients receiving immunotherapies. Despite the wide range of techniques used, to date these assays have not shown consistent results among clinical trials and failed to define surrogate markers of clinical efficacy to antitumor vaccines. Multiparameter flow cytometry- (FCM-) based assays combining different phenotypic and functional markers have been developed in the past decade for informative and longitudinal analysis of polyfunctional T-cells. These technologies were designed to address the complexity and functional heterogeneity of cancer biology and cellular immunity and to define biomarkers predicting clinical response to anticancer treatment. So far, there is still a lack of standardization of some of these immunological tests. The aim of this review is to overview the latest technologies for immune monitoring and to highlight critical steps involved in some of the FCM-based cellular immune assays. In particular, our laboratory is focused on melanoma vaccine research and thus our main goal was the validation of a functional multiparameter test (FMT) combining different functional and lineage markers to be applied in clinical trials involving patients with melanoma. PMID:24195078
Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.
Watanabe, Masaru; Uehara, Yuri; Yamashita, Namiko; Fujimura, Yuu; Nishio, Kaori; Sawada, Takeshi; Takeda, Kazuo; Koizumi, Fumiaki; Koh, Yasuhiro
2014-03-01
The presence and number of circulating tumor cells (CTCs) in the blood of patients with solid tumors are predictive of their clinical outcomes. To date, the CellSearch system is the only US Food and Drug Administration-approved CTC enumeration system for advanced breast, prostate, and colon cancers. However, sensitivity issues due to epithelial cellular adhesion molecule (EpCAM)-based enrichment and limited capability for subsequent molecular analysis must be addressed before CTCs can be used as predictive markers in the clinical setting. We have developed a multicolor CTC detection system using cross-contamination-free flow cytometry, which permits the enumeration and characterization of CTCs for multiple molecular analyses. Tumor cell lines with different expression levels of EpCAM were spiked into peripheral blood obtained from healthy donors. Spike-in samples were negatively enriched using anti-CD45-coated magnetic beads to remove white blood cells, and this was followed by fixation and labeling with CD45-Alexa Fluor 700, EpCAM-phycoerythrin, cytokeratin (CK)-fluorescein isothiocyanate antibodies, and/or 7-aminoactinomycin D for nuclei staining. Excellent detection (slope = 0.760-0.888) and a linear performance (R(2) = 0.994-0.998) were noted between the observed and expected numbers of tumor cells, independent of EpCAM expression. The detection rate was markedly higher than that obtained using the CellSearch system, suggesting the superior sensitivity of our system in detecting EpCAM- tumor cells. Additionally, the incorporation of an epithelial-mesenchymal transition (EMT) marker allowed us to detect EpCAM-/CK- cells and EMT-induced tumor cells. Taken together, our multicolor CTC detection system may be highly efficient in detecting previously unrecognized populations of CTCs.
Salvagno, Camilla; de Visser, Karin E
2016-01-01
It is well established that tumors evolve together with nonmalignant cells, such as fibroblasts, endothelial cells, and immune cells. These cells constantly entangle and interact with each other creating the tumor microenvironment. Immune cells can exert both tumor-promoting and tumor-protective functions. Detailed phenotypic and functional characterization of intra-tumoral immune cell subsets has become increasingly important in the field of cancer biology and cancer immunology. In this chapter, we describe a method for isolation of viable and pure immune cell subsets from freshly isolated murine solid tumors and organs. First, we describe a protocol for the generation of single-cell suspensions from tumors and organs using mechanical and enzymatic strategies. In addition, we describe how immune cell subsets can be purified by consecutive magnetic cell sorting and multi-parameter flow cytometry-based cell sorting.
Salvagno, Camilla; de Visser, Karin E
2016-01-01
It is well established that tumors evolve together with nonmalignant cells, such as fibroblasts, endothelial cells, and immune cells. These cells constantly entangle and interact with each other creating the tumor microenvironment. Immune cells can exert both tumor-promoting and tumor-protective functions. Detailed phenotypic and functional characterization of intra-tumoral immune cell subsets has become increasingly important in the field of cancer biology and cancer immunology. In this chapter, we describe a method for isolation of viable and pure immune cell subsets from freshly isolated murine solid tumors and organs. First, we describe a protocol for the generation of single-cell suspensions from tumors and organs using mechanical and enzymatic strategies. In addition, we describe how immune cell subsets can be purified by consecutive magnetic cell sorting and multi-parameter flow cytometry-based cell sorting. PMID:27581019
Ribeiro, Andreia; Ritter, Thomas; Griffin, Matthew; Ceredig, Rhodri
2016-09-01
Human bone marrow-derived mesenchymal stromal/stem cells (MSC) have well-documented modulatory effects on multiple immune cell types. Although these effects are linked to their therapeutic benefit in diverse diseases, a reliable, quantitative assay of the immunomodulatory potency of individual human MSC preparations is lacking. The aims of this study were to develop an optimised rapid turnaround, flow cytometry-based whole-blood assay to monitor MSC potency and to validate its application to MSC immunomodulation. A protocol for short-term LPS stimulation of anti-coagulated whole blood samples followed by combined surface CD45/CD14 and intracellular TNF-α staining was initially developed for analysis on a 4 colour desktop cytometer. Optimal monocyte activation was dependent on the presence of extracellular calcium ions thereby precluding the use of EDTA and sodium citrate as anticoagulants. Optimal assay conditions proved to be 1ng/mL ultrapure-LPS added to 10-fold diluted, heparin anti-coagulated whole blood incubated for 6h at 37°C. Under these conditions, addition of human bone marrow-derived MSC (hBM-MSC) from multiple donors resulted in a reproducible, dose-dependent inhibition of LPS-stimulated monocyte TNF-α expression. We conclude that this protocol represents a practical, quantitative assay of a clinically relevant functional effect of hBM-MSCs as well as other immunomodulatory agents. PMID:27451032
Li, Zengji; Ling, Loni; Liu, Xiaohui; Laus, Reiner; Delcayre, Alain
2010-10-01
A flow cytometry-based immuno-titration titer assay was established to determine infectious unit (IU) and transducing unit (TU) of modified vaccinia Ankara (MVA) virus vectors. This titration method enumerates infected cells by measuring the expression of viral protein for IU and transgene protein for TU in individual cells after staining with fluorophore-conjugated antibodies. It presents many advantages over standard virus titration approaches, such as TCID(50) or plaque assay, for its convenience, rapidity and accuracy as illustrated by excellent assay linearity and reproducibility. Importantly, the IU and the TCID(50) assays generated similar batch-specific titer values when testing varied MVA-derived virus preparations. Assay development revealed that the post-infection time at which viral protein expression is evaluated, host cell type, and blocking the formation and release of progeny virion with nocodazole, an anti-microtubule agent or rifampin, a specific vaccinia virus assembly inhibitor, are critical parameters for the precision, robustness, and accuracy of IU titer determination. An added advantage of this assay is that it enables the concurrent determination of IU and transducing units (TU) by measuring the expression of a transgene product when testing recombinant viruses. The latter was demonstrated using a MVA vector carrying a human HER-2 gene fragment as model. Hence, this assay is very versatile in that it can be used to determine IU as well as multiple TU titers simultaneously. Furthermore, it can readily be adapted to other poxvirus vectors.
Stevens, R; Coates, E; Street, J; Cook, E; Darke, C
2013-08-01
Hypersensitivity reactions to the drug abacavir, used to treat HIV/AIDS patients, is associated with possession of HLA-B*57:01. We have carefully assessed two commercially available HLA-B57/B58 murine monoclonal antibodies [0196HA and BIH0243 (One Lambda Inc.)] in a simple flow cytometry-based assay. The evaluation involved tests on 228 reference and random samples covering 91% of all WHO recognized HLA-A, B and C specificities. These involved donors with six different HLA-B*57 alleles and included 19 examples of B*57:01. Both antibodies unambiguously detected B57, but there were small difference in their reactivity against B57-positive non-B*57:01 samples. Importantly, there was no reactivity against B57/B58-negative samples. The possible amino acid motifs involved in the reactivity of these antibodies with B57/B58 were delineated. Thus, HLA-B57/B58, normally present in <10% of patients, can be easily recognized using these two antibodies and further tested by a DNA-based typing method to identify B*57:01.
Silva-dos-Santos, Priscila Pinto; Barros, Geisa Baptista; Mineo, José Roberto; de Oliveira Silva, Deise Aparecida; Menegaz, Mauro Hygino Weinert; Serufo, José Carlos; Dietze, Reynaldo; Martins-Filho, Olindo de Assis; Lemos, Elenice Moreira
2012-04-30
In the present study we evaluated the performance of a flow cytometry-based algorithm as a new serological approach to detect antibodies to T. gondii and specific IgG avidity to diagnose acute toxoplasmosis. The results showed that using FC-AFTA-IgM assay, all serum samples from patients with acute toxoplasmosis demonstrated seropositivity, whereas 90% of patients with chronic infection and 100% of non-infected individuals presented negative results. Thus, only 10% of patients with chronic toxoplasmosis showed residual IgM, in contrast with other methodologies used to diagnosis acute toxoplasmosis. On the order hand, FC-AFTA-IgG assay as well as FC-AFTA-IgG subclasses is unlikely to discriminate acute from chronic toxoplasmosis. We have also evaluated the performance of FC-AFTA-IgG avidity as a tool to exclude chronic toxoplasmosis in patients with positive FC-AFTA-IgM. Our data showed an excellent performance of FC-AFTA-IgG avidity employing the cut-off of 60% for Avidity Index (AI) with sensitivity and specificity of 100%. All serum samples from patients presenting acute toxoplasmosis showed low avidity index (AI≤60%), whereas all chronic patients showed high avidity index (AI>60%). The outstanding performance indexes of this novel flow cytometry-based algorithm support its use as a non-conventional alternative serological approach to diagnose human acute toxoplasmosis.
Righetto, L; Zaman, R U; Mahmud, Z H; Bertuzzo, E; Mari, L; Casagrandi, R; Gatto, M; Islam, S; Rinaldo, A
2015-08-01
Presence of Vibrio cholerae serogroups O1 and O139 in the waters of the rural area of Matlab, Bangladesh, was investigated with quantitative measurements performed with a portable flow cytometer. The relevance of this work relates to the testing of a field-adapted measurement protocol that might prove useful for cholera epidemic surveillance and for validation of mathematical models. Water samples were collected from different water bodies that constitute the hydrological system of the region, a well-known endemic area for cholera. Water was retrieved from ponds, river waters, and irrigation canals during an inter-epidemic time period. Each sample was filtered and analysed with a flow cytometer for a fast determination of V. cholerae cells contained in those environments. More specifically, samples were treated with O1- and O139-specific antibodies, which allowed precise flow-cytometry-based concentration measurements. Both serogroups were present in the environmental waters with a consistent dominance of V. cholerae O1. These results extend earlier studies where V. cholerae O1 and O139 were mostly detected during times of cholera epidemics using standard culturing techniques. Furthermore, our results confirm that an important fraction of the ponds' host populations of V. cholerae are able to self-sustain even when cholera cases are scarce. Those contaminated ponds may constitute a natural reservoir for cholera endemicity in the Matlab region. Correlations of V. cholerae concentrations with environmental factors and the spatial distribution of V. cholerae populations are also discussed. PMID:25496520
Buzatu, Dan A.; Moskal, Ted J.; Williams, Anna J.; Cooper, Willie Mae; Mattes, William B.; Wilkes, Jon G.
2014-01-01
Foodborne illnesses occur in both industrialized and developing countries, and may be increasing due to rapidly evolving food production practices. Yet some primary tools used to assess food safety are decades, if not centuries, old. To improve the time to result for food safety assessment a sensitive flow cytometer based system to detect microbial contamination was developed. By eliminating background fluorescence and improving signal to noise the assays accurately measure bacterial load or specifically identify pathogens. These assays provide results in minutes or, if sensitivity to one cell in a complex matrix is required, after several hours enrichment. Conventional assessments of food safety require 48 to 56 hours. The assays described within are linear over 5 orders of magnitude with results identical to culture plates, and report live and dead microorganisms. This system offers a powerful approach to real-time assessment of food safety, useful for industry self-monitoring and regulatory inspection. PMID:24718659
Chen, Jun; Young, Susan M.; Allen, Chris; Seeber, Andrew; Péli-Gulli, Marie-Pierre; Panchaud, Nicolas; Waller, Anna; Ursu, Oleg; Yao, Tuanli; Golden, Jennifer E.; Strouse, J. Jacob; Carter, Mark B.; Kang, Huining; Bologa, Cristian G.; Foutz, Terry D.; Edwards, Bruce S.; Peterson, Blake R.; Aubé, Jeffrey; Werner-Washburne, Margaret; Loewith, Robbie J.; De Virgilio, Claudio; Sklar, Larry A.
2012-01-01
TOR (target of rapamycin) is a serine/threonine kinase, evolutionarily conserved from yeast to human, which functions as a fundamental controller of cell growth. The moderate clinical benefit of rapamycin in mTOR-based therapy of many cancers favors the development of new TOR inhibitors. Here we report a high throughput flow cytometry multiplexed screen using five GFP-tagged yeast clones that represent the readouts of four branches of the TORC1 signaling pathway in budding yeast. Each GFP-tagged clone was differentially color-coded and the GFP signal of each clone was measured simultaneously by flow cytometry, which allows rapid prioritization of compounds that likely act through direct modulation of TORC1 or proximal signaling components. A total of 255 compounds were confirmed in dose-response analysis to alter GFP expression in one or more clones. To validate the concept of the high throughput screen, we have characterized CID 3528206, a small molecule most likely to act on TORC1 as it alters GFP expression in all five GFP clones in an analogous manner to rapamycin. We have shown that CID 3528206 inhibited yeast cell growth, and that CID 3528206 inhibited TORC1 activity both in vitro and in vivo with EC50s of 150 nM and 3.9 μM, respectively. The results of microarray analysis and yeast GFP collection screen further support the notion that CID 3528206 and rapamycin modulate similar cellular pathways. Together, these results indicate that the HTS has identified a potentially useful small molecule for further development of TOR inhibitors. PMID:22260433
Quadrini, Karen J; Hegelund, Anne Charlotte; Cortes, Kasia E; Xue, Chengsen; Kennelly, Susan M; Ji, Hong; Högerkorp, Carl-Magnus; Mc Closkey, Thomas W
2016-03-01
The C5a/C5a receptor (C5aR) pathway, a key component in the proinflammatory immune response, is an attractive therapeutic target since its dysregulation is implicated in a variety of autoimmune and inflammatory disorders. The objective of the present study was to validate a receptor occupancy (RO) assay for a human anti-C5aR monoclonal antibody drug candidate, NNC0215-0384 (NN0384). This flow cytometry-based assay measures the percentage (%), median fluorescence intensity (MFI), and molecules of equivalent soluble fluorochrome (MESF) of NN0384 binding to its target cells, neutrophils and monocytes, in whole blood from normal healthy donors and rheumatoid arthritis (RA) patients with clinically active disease. The validation parameters assessed included postcollection and postprocessing sample stability, intra- and interassay precision, an analyst-to-analyst comparison, a comparison of normal healthy donor and RA patient sample postcollection stability, and a laboratory-to-laboratory comparison and assay transfer. The cumulative results indicate that the assay was reproducible, met the clearly defined acceptance criteria for the validation parameters tested, and was transferable to another laboratory. In conclusion, this RO assay is suitable for use to accrue pharmacodynamic biomarker data in a multicenter, global clinical trial. PMID:26084468
Technology Transfer Automated Retrieval System (TEKTRAN)
Recent developments in spectrally encoded microspheres (SEMs)-based technologies provide high multiplexing possibilities. Most SEMs-based assays required a flow cytometer with sophisticated fluidics and optics. The new imaging superparamagnetic SEMs-based platform transports SEMs with considerably ...
NASA Astrophysics Data System (ADS)
Kong, Lingjie; Tang, Jianyong; Cui, Meng
2016-03-01
In vivo flow cytometry has found numerous applications in biology and pharmacology. However, conventional cytometry does not provide the detailed morphological information that is needed to fully determine the phenotype of individual circulating cells. Imaging cytometry, capable of visualizing the morphology and dynamics of the circulating cells at high spatiotemporal resolution, is highly desired. Current wide-field based image cytometers are limited in the imaging depth and provide only two-dimensional resolution. For deep tissue imaging, laser scanning two-photon fluorescence microscopy (TPM) is widely adopted. However, for applications in flow cytometry, the axial scanning speed of current TPMs is inadequate to provide high-speed cross-sectional imaging of vasculature. We have integrated an optical phase-locked ultrasound lens into a standard TPM and achieved microsecond-scale axial scanning. With a galvo scanner for transverse scanning, we achieved kHz cross-sectional frame rate. Here we report its applications for in vivo deformability cytometry and in vivo imaging flow cytometry, and demonstrate the capability of imaging dynamical morphologies of flowing cells, distinguishing cells and cellular clusters, and simultaneously quantifying different cell populations based on their fluorescent labels.
Meimaridou, Anastasia; Haasnoot, Willem; Shelver, Weilin L; Franek, Milan; Nielen, Michel W F
2013-01-01
Recent developments in spectrally encoded microspheres (SEMs)-based technologies provide high multiplexing possibilities. Most SEMs-based assays require a flow cytometer with sophisticated fluidics and optics. A new imaging super-paramagnetic SEMs-based alternative platform transports SEMs with considerably less fluid volume into a measuring chamber. Once there SEMs are held in a monolayer by a magnet. Light-emitting diodes (LEDs) are focused on the chamber to illuminate the SEMs - instead of lasers and they are imaged by a charge-coupled device (CCD) detector, offering a more compact sized, transportable and affordable system. The feasibility of utilising this system to develop a 3-plex SEMs-based imaging immunoassay (IMIA) for the screening of persistent organic pollutants (POPs) was studied. Moreover the performance characteristics of 3-plex IMIA were critically compared with the conventional 3-plex flow cytometric immunoassay (FCIA). Both SEM technologies have potential for the multiplex analysis of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs) in buffer and fish extract with insignificant differences in assay sensitivities. Furthermore, we developed a faster and simpler, modified QuEChERS-like generic POPs extraction from tilapia fillet using sodium hydrogen carbonate as one of the salt additives and dispersive solid-phase extraction (dSPE) as a clean-up. Finally, a preliminary in-house validation using 40 different blank and spiked tilapia fillet samples was performed in both systems and the results obtained were critically compared. The lower-cost imaging SEMs-based system performed similarly to the original flow cytometer and, in combination with the new quicker QuEChERS-like extraction, it has high potential for future rapid screening of POPs in several other sample matrices such as other fish species, vegetable refined oils and environmental samples.
Korfali, Nadia; Srsen, Vlastimil; Waterfall, Martin; Batrakou, Dzmitry G; Pekovic, Vanja; Hutchison, Christopher J; Schirmer, Eric C
2011-01-01
Disruption of cell cycle regulation is one mechanism proposed for how nuclear envelope protein mutation can cause disease. Thus far only a few nuclear envelope proteins have been tested/found to affect cell cycle progression: to identify others, 39 novel nuclear envelope transmembrane proteins were screened for their ability to alter flow cytometry cell cycle/DNA content profiles when exogenously expressed. Eight had notable effects with seven increasing and one decreasing the 4N:2N ratio. We subsequently focused on NET4/Tmem53 that lost its effects in p53(-/-) cells and retinoblastoma protein-deficient cells. NET4/TMEM53 knockdown by siRNA altered flow cytometry cell cycle/DNA content profiles in a similar way as overexpression. NET4/TMEM53 knockdown did not affect total retinoblastoma protein levels, unlike nuclear envelope-associated proteins Lamin A and LAP2α. However, a decrease in phosphorylated retinoblastoma protein was observed along with a doubling of p53 levels and a 7-fold increase in p21. Consequently cells withdrew from the cell cycle, which was confirmed in MRC5 cells by a drop in the percentage of cells expressing Ki-67 antigen and an increase in the number of cells stained for ß-galactosidase. The ß-galactosidase upregulation suggests that cells become prematurely senescent. Finally, the changes in retinoblastoma protein, p53, and p21 resulting from loss of NET4/Tmem53 were dependent upon active p38 MAP kinase. The finding that roughly a fifth of nuclear envelope transmembrane proteins screened yielded alterations in flow cytometry cell cycle/DNA content profiles suggests a much greater influence of the nuclear envelope on the cell cycle than is widely held.
Rouget, Raphael; Auclair, Yannick; Loignon, Martin; Affar, El Bachir; Drobetsky, Elliot A
2008-02-29
In response to diverse genotoxic stimuli (e.g. UV and cisplatin), the mitogen-activated protein kinases ERK1/2, JNK1/2, and p38alpha/beta become rapidly phosphorylated and in turn activate multiple downstream effectors that modulate apoptosis and/or growth arrest. Furthermore, previous lines of evidence have strongly suggested that ERK1/2 and JNK1/2 participate in global-genomic nucleotide excision repair, a critical antineoplastic pathway that removes helix-distorting DNA adducts induced by a variety of mutagenic agents, including UV. To rigorously evaluate the potential role of mitogen-activated protein kinases in global-genomic nucleotide excision repair, various human cell strains (primary skin fibroblasts, primary lung fibroblasts, and HCT116 colon carcinoma cells) were treated with highly specific chemical inhibitors, which, following UV exposure, (i) abrogated the capacities of ERK1/2, JNK1/2, or p38alpha/beta to phosphorylate specific downstream effectors and (ii) characteristically modulated cellular proliferation, clonogenic survival, and/or apoptosis. A highly sensitive flow cytometry-based nucleotide excision repair assay recently optimized and validated in our laboratory was then employed to directly demonstrate that the kinetics of UV DNA photoadduct repair are highly similar in mock-treated versus mitogen-activated protein kinase inhibitor-treated cells. These data on primary and tumor cells treated with pharmacological inhibitors were fully corroborated by repair studies using (i) short hairpin RNA-mediated knockdown of ERK1/2 or JNK1/2 in human U2OS osteosarcoma cells and (ii) expression of a dominant negative p38alpha mutant in human primary lung fibroblasts. Our results provide solid evidence for the first time, in disaccord with a burgeoning perception, that mitogen-activated protein kinase signaling does not influence the efficiency of human global-genomic nucleotide excision repair.
Torres, Alex José Leite; Brígido, Luis Fernando de Macedo; Abrahão, Marcos Herculano Nunes; Angelo, Ana Luiza Dias; de Jesus Ferreira, Gilcivaldo; Coelho, Luana Portes; Ferreira, João Leandro; Jorge, Célia Regina Mayoral Pedroso; Netto, Eduardo Martins; Brites, Carlos
2015-01-01
Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a flow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identified by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identification of X4 and R5 tropic viruses was attempted by geno2pheno algorithm. There was a high degree of concordance between cytometry-based tropism assay and geno2pheno algorithm in determination of HIV-1 tropism. Cytometry-based tropism assay demonstrated higher sensitivity and specificity in comparison to geno2pheno, which was used as a gold-standard. One sample could not be amplified by geno2pheno method, but was classified as duotropic by cytometry-based tropism assay. We did not find any association between CD4+ count or plasma HIV-1 RNA viral load and tropism results. The overall performances of cytometry-based tropism assay and geno2pheno assay were almost identical in determination of HIV-1 tropism.
Flow analysis system and method
NASA Technical Reports Server (NTRS)
Hill, Wayne S. (Inventor); Barck, Bruce N. (Inventor)
1998-01-01
A non-invasive flow analysis system and method wherein a sensor, such as an acoustic sensor, is coupled to a conduit for transmitting a signal which varies depending on the characteristics of the flow in the conduit. The signal is amplified and there is a filter, responsive to the sensor signal, and tuned to pass a narrow band of frequencies proximate the resonant frequency of the sensor. A demodulator generates an amplitude envelope of the filtered signal and a number of flow indicator quantities are calculated based on variations in amplitude of the amplitude envelope. A neural network, or its equivalent, is then used to determine the flow rate of the flow in the conduit based on the flow indicator quantities.
Vortex methods for separated flows
NASA Technical Reports Server (NTRS)
Spalart, Philippe R.
1988-01-01
The numerical solution of the Euler or Navier-Stokes equations by Lagrangian vortex methods is discussed. The mathematical background is presented and includes the relationship with traditional point-vortex studies, convergence to smooth solutions of the Euler equations, and the essential differences between two and three-dimensional cases. The difficulties in extending the method to viscous or compressible flows are explained. Two-dimensional flows around bluff bodies are emphasized. Robustness of the method and the assessment of accuracy, vortex-core profiles, time-marching schemes, numerical dissipation, and efficient programming are treated. Operation counts for unbounded and periodic flows are given, and two algorithms designed to speed up the calculations are described.
Vortex methods for separated flows
NASA Technical Reports Server (NTRS)
Spalart, Philippe R.
1988-01-01
The numerical solution of the Euler or Navier-Stokes equations by Lagrangian vortex methods is discussed. The mathematical background is presented in an elementary fashion and includes the relationship with traditional point-vortex studies, the convergence to smooth solutions of the Euler equations, and the essential differences between two- and three-dimensional cases. The difficulties in extending the method to viscous or compressible flows are explained. The overlap with the excellent review articles available is kept to a minimum and more emphasis is placed on the area of expertise, namely two-dimensional flows around bluff bodies. When solid walls are present, complete mathematical models are not available and a more heuristic attitude must be adopted. The imposition of inviscid and viscous boundary conditions without conformal mappings or image vortices and the creation of vorticity along solid walls are examined in detail. Methods for boundary-layer treatment and the question of the Kutta condition are discussed. Practical aspects and tips helpful in creating a method that really works are explained. The topics include the robustness of the method and the assessment of accuracy, vortex-core profiles, timemarching schemes, numerical dissipation, and efficient programming. Calculations of flows past streamlined or bluff bodies are used as examples when appropriate.
Flow Cytometry-Based Classification in Cancer Research: A View on Feature Selection
Hassan, S. Sakira; Ruusuvuori, Pekka; Latonen, Leena; Huttunen, Heikki
2015-01-01
In this paper, we study the problem of feature selection in cancer-related machine learning tasks. In particular, we study the accuracy and stability of different feature selection approaches within simplistic machine learning pipelines. Earlier studies have shown that for certain cases, the accuracy of detection can easily reach 100% given enough training data. Here, however, we concentrate on simplifying the classification models with and seek for feature selection approaches that are reliable even with extremely small sample sizes. We show that as much as 50% of features can be discarded without compromising the prediction accuracy. Moreover, we study the model selection problem among the ℓ1 regularization path of logistic regression classifiers. To this aim, we compare a more traditional cross-validation approach with a recently proposed Bayesian error estimator. PMID:27081305
Krylov methods for compressible flows
NASA Technical Reports Server (NTRS)
Tidriri, M. D.
1995-01-01
We investigate the application of Krylov methods to compressible flows, and the effect of implicit boundary conditions on the implicit solution of nonlinear problems. Two defect-correction procedures, namely, approximate factorization (AF) for structured grids and ILU/GMRES for general grids, are considered. Also considered here are Newton-Krylov matrix-free methods that we combined with the use of mixed discretization schemes in the implicitly defined Jacobian and its preconditioner. Numerical experiments that show the performance of our approaches are then presented.
Insertable fluid flow passage bridgepiece and method
Jones, Daniel O.
2000-01-01
A fluid flow passage bridgepiece for insertion into an open-face fluid flow channel of a fluid flow plate is provided. The bridgepiece provides a sealed passage from a columnar fluid flow manifold to the flow channel, thereby preventing undesirable leakage into and out of the columnar fluid flow manifold. When deployed in the various fluid flow plates that are used in a Proton Exchange Membrane (PEM) fuel cell, bridgepieces of this invention prevent mixing of reactant gases, leakage of coolant or humidification water, and occlusion of the fluid flow channel by gasket material. The invention also provides a fluid flow plate assembly including an insertable bridgepiece, a fluid flow plate adapted for use with an insertable bridgepiece, and a method of manufacturing a fluid flow plate with an insertable fluid flow passage bridgepiece.
Gas flow meter and method for measuring gas flow rate
Robertson, Eric P.
2006-08-01
A gas flow rate meter includes an upstream line and two chambers having substantially equal, fixed volumes. An adjustable valve may direct the gas flow through the upstream line to either of the two chambers. A pressure monitoring device may be configured to prompt valve adjustments, directing the gas flow to an alternate chamber each time a pre-set pressure in the upstream line is reached. A method of measuring the gas flow rate measures the time required for the pressure in the upstream line to reach the pre-set pressure. The volume of the chamber and upstream line are known and fixed, thus the time required for the increase in pressure may be used to determine the flow rate of the gas. Another method of measuring the gas flow rate uses two pressure measurements of a fixed volume, taken at different times, to determine the flow rate of the gas.
Effect of flow fluctuations and nonflow on elliptic flow methods
Ollitrault, Jean-Yves; Poskanzer, Arthur M.; Voloshin, Sergei A.
2009-04-16
We discuss how the different estimates of elliptic flow are influenced by flow fluctuations and nonflow effects. It is explained why the event-plane method yields estimates between the two-particle correlation methods and the multiparticle correlation methods. It is argued that nonflow effects and fluctuations cannot be disentangled without other assumptions. However, we provide equations where, with reasonable assumptions about fluctuations and nonflow, all measured values of elliptic flow converge to a unique mean v_2,PP elliptic flow in the participant plane and, with a Gaussian assumption on eccentricity fluctuations, can be converted to the mean v_2,RP in the reaction plane. Thus, the 20percent spread in observed elliptic flow measurements from different analysis methods is no longer mysterious.
On the Methods to Measure Powder Flow.
Tan, Geoffrey; Morton, David A V; Larson, Ian
2015-01-01
The flow of powders can often play a critical role in the manufacturing of pharmaceutical products. Many of these processes require good, consistent and predictable flow of powders to ensure continuous production of pharmaceutical dosages and to ensure their quality. Therefore, the flow of powders is of paramount importance to the pharmaceutical industry and thus the measuring and evaluating of powder flow is of utmost importance. At present, there are numerous methods in which the flow of powders can be measured. However, due to the complex and environment-dependent nature of powders, no one method exists that is capable of providing a complete picture of the behaviour of powders under dynamic conditions. Some of the most commonly applied methods to measure the flow of powders include: density indices, such as the Carr index and Hausner ratio, powder avalanching, the angle of repose (AOR), flow through an orifice, powder rheometry and shear cell testing.
Williams, Anna J; Cooper, Willie M; Summage-West, Christine V; Sims, Lillie M; Woodruff, Robert; Christman, Jessica; Moskal, Ted J; Ramsaroop, Shawn; Sutherland, John B; Alusta, Pierre; Wilkes, Jon G; Buzatu, Dan A
2015-12-23
The Bacteriological Analytical Manual (BAM) method currently used by the United States Food and Drug Administration (FDA) to detect Escherichia coli O157:H7 in spinach was systematically compared to a new flow cytometry based method. This Food and Drug Administration (FDA) level 2 external laboratory validation study was designed to determine the latter method's sensitivity and speed for analysis of this pathogen in raw spinach. Detection of target cell inoculations with a low cell count is critical, since enterohemorrhagic strains of E. coli require an infective dose of as few as 10 cells (Schmid-Hempel and Frank, 2007). Although, according to the FDA, the infectious dose is unknown (Food and Drug Administration, 1993). Therefore, the inoculation level into the spinach, a total of 2.0±2.6 viable E. coli O157 cells, was specified to yield between 25% and 75% detection by the new method, out of 20 samples (10 positives and 10 negatives). This criterion was met in that the new method detected 60% of the nominally positive samples; the corresponding sensitivity of the reference method was 50%. For both methods the most likely explanation for false negatives was that no viable cells were actually introduced into the sample. In this validation study, the flow cytometry method was equal to the BAM in sensitivity and far superior in speed.
A novel flow cytometric-based method to measure kinase inhibition in sputum from COPD subjects
Nicholson, G C; Holloway, R A; Leaker, B R; Kilty, I; Salganik, M; Tan, L; Barnes, P J; Donnelly, L E
2016-01-01
Introduction Janus kinases (JAKs) regulate inflammatory gene expression through phosphorylation of signal transducer and activator of transcription (STAT) proteins. Expression of STAT proteins is increased in chronic obstructive pulmonary disease (COPD), and may be involved in driving chronic inflammation. Oral JAK inhibitors are effective as anti-inflammatory therapy but exhibit dose-limiting adverse effects. Development of inhaled compounds would be enhanced by robust biomarkers that directly reflect the anti-inflammatory and pharmacological activity in the lung. Methods A novel flow cytometry assay was developed to measure STAT1 phosphorylation in sputum inflammatory cells. The standard sputum processing method was refined to improve sputum cell viability. The flow cytometric assay was used to assess the reproducibility of the measurement of STAT1 phosphorylation and the in vitro activity of a pan JAK-inhibitor on three separate visits in patients with COPD. Results Upregulation of STAT1 phosphorylation was measured following in vitro IFNγ stimulation of sputum macrophages (stimulated/unstimulated ratio 1.57; p<0.00001). Upregulation was inhibited following in vitro preincubation with a pan JAK-inhibitor (inhibited+stimulated/unstimulated ratio 0.97). STAT1 phosphorylation activity could only be measured in macrophages. Conclusions Sputum from patients with COPD can be used to reproducibly measure phospho-STAT expression in sputum macrophages. The flow cytometry-based method can be used to evaluate kinase inhibitors in vitro and subsequently in ex vivo studies. The assay is particularly useful for the assessment of inhaled compounds where whole blood assays may not be relevant. PMID:27403320
Spectral methods for compressible reactive flows
NASA Astrophysics Data System (ADS)
Gottlieb, David; Gottlieb, Sigal
2005-01-01
High order simulations are necessary in order to capture fine details in resolving supersonic reactive flows. However, high Mach number compressible flows feature sharp gradients and discontinuities, which present a challenge to successful simulations using high order methods. Spectral methods have proven a powerful tool in simulation of incompressible turbulent flows, and recent advances allow the application of spectral methods to compressible reactive flows. We review the recent advances in the theory and application of spectral methods which allow stable computations of discontinuous phenomena, and the recovery of high order information via postprocessing, and present applications of high Mach number reactive flows. To cite this article: D. Gottlieb, S. Gottlieb, C. R. Mecanique 333 (2005).
Spectral methods for inviscid, compressible flows
NASA Technical Reports Server (NTRS)
Hussaini, M. Y.; Salas, M. D.; Zang, T. A.
1983-01-01
Report developments in the application of spectral methods to two dimensional compressible flows are reviewed. A brief introduction to spectral methods -- their history and especially their implementation -- is provided. The stress is on those techniques relevant to transonic flow computation. The spectral multigrid iterative methods are discussed with application to the transonic full potential equation. Discontinuous solutions of the Euler equations are considered. The key element is the shock fitting technique which is briefly explained.
Computational methods for ideal compressible flow
NASA Technical Reports Server (NTRS)
Vanleer, B.
1983-01-01
Conservative dissipative difference schemes for computing one dimensional flow are introduced, and the recognition and representation of flow discontinuities are discussed. Multidimensional methods are outlined. Second order finite volume schemes are introduced. Conversion of difference schemes for a single linear convection equation into schemes for the hyperbolic system of the nonlinear conservation laws of ideal compressible flow is explained. Approximate Riemann solvers are presented. Monotone initial value interpolation; and limiters, switches, and artificial dissipation are considered.
Solving functional flow equations with pseudospectral methods
NASA Astrophysics Data System (ADS)
Borchardt, J.; Knorr, B.
2016-07-01
We apply pseudospectral methods to integrate functional flow equations with high accuracy, extending earlier work on functional fixed point equations [J. Borchardt and B. Knorr, Phys. Rev. D 91, 105011 (2015)]. The advantages of our method are illustrated with the help of two classes of models: first, to make contact with literature, we investigate flows of the O (N ) model in three dimensions, for N =1 , 4 and in the large N limit. For the case of a fractal dimension, d =2.4 , and N =1 , we follow the flow along a separatrix from a multicritical fixed point to the Wilson-Fisher fixed point over almost 13 orders of magnitude. As a second example, we consider flows of bounded quantum-mechanical potentials, which can be considered as a toy model for Higgs inflation. Such flows pose substantial numerical difficulties, and represent a perfect test bed to exemplify the power of pseudospectral methods.
Tomographic methods in flow diagnostics
NASA Technical Reports Server (NTRS)
Decker, Arthur J.
1993-01-01
This report presents a viewpoint of tomography that should be well adapted to currently available optical measurement technology as well as the needs of computational and experimental fluid dynamists. The goals in mind are to record data with the fastest optical array sensors; process the data with the fastest parallel processing technology available for small computers; and generate results for both experimental and theoretical data. An in-depth example treats interferometric data as it might be recorded in an aeronautics test facility, but the results are applicable whenever fluid properties are to be measured or applied from projections of those properties. The paper discusses both computed and neural net calibration tomography. The report also contains an overview of key definitions and computational methods, key references, computational problems such as ill-posedness, artifacts, missing data, and some possible and current research topics.
Asymptotic methods for internal transonic flows
NASA Technical Reports Server (NTRS)
Adamson, T. C., Jr.; Messiter, A. F.
1989-01-01
For many internal transonic flows of practical interest, some of the relevant nondimensional parameters typically are small enough that a perturbation scheme can be expected to give a useful level of numerical accuracy. A variety of steady and unsteady transonic channel and cascade flows is studied with the help of systematic perturbation methods which take advantage of this fact. Asymptotic representations are constructed for small changes in channel cross-section area, small flow deflection angles, small differences between the flow velocity and the sound speed, small amplitudes of imposed oscillations, and small reduced frequencies. Inside a channel the flow is nearly one-dimensional except in thin regions immediately downstream of a shock wave, at the channel entrance and exit, and near the channel throat. A study of two-dimensional cascade flow is extended to include a description of three-dimensional compressor-rotor flow which leads to analytical results except in thin edge regions which require numerical solution. For unsteady flow the qualitative nature of the shock-wave motion in a channel depends strongly on the orders of magnitude of the frequency and amplitude of impressed wall oscillations or fluctuations in back pressure. One example of supersonic flow is considered, for a channel with length large compared to its width, including the effect of separation bubbles and the possibility of self-sustained oscillations. The effect of viscosity on a weak shock wave in a channel is discussed.
An exact inverse method for subsonic flows
NASA Technical Reports Server (NTRS)
Daripa, Prabir
1988-01-01
A new inverse method for the aerodynamic design of airfoils is presented for subcritical flows. The pressure distribution in this method can be prescribed as a function of the arclength of the still unknown body. It is shown that this inverse problem is mathematically equivalent to solving only one nonlinear boundary value problem subject to known Dirichlet data on the boundary.
Lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk.
Fernandes, Ana C; Duarte, Carla M; Cardoso, Filipe A; Bexiga, Ricardo; Cardoso, Susana; Freitas, Paulo P
2014-08-21
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk.
Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
Fernandes, Ana C.; Duarte, Carla M.; Cardoso, Filipe A.; Bexiga, Ricardo.; Cardoso, Susana.; Freitas, Paulo P.
2014-01-01
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk. PMID:25196163
Lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk.
Fernandes, Ana C; Duarte, Carla M; Cardoso, Filipe A; Bexiga, Ricardo; Cardoso, Susana; Freitas, Paulo P
2014-01-01
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved in a compact and modular format. This feature can be combined with magnetic detection, where magnetoresistive sensors can be integrated within microfluidic channels to detect magnetically labelled cells. This work describes a platform for in-flow detection of magnetically labelled cells with a magneto-resistive based cell cytometer. In particular, we present an example for the validation of the platform as a magnetic counter that identifies and quantifies Streptococcus agalactiae in milk. PMID:25196163
Semiempirical methods for computing turbulent flows
NASA Astrophysics Data System (ADS)
Belov, I. A.; Ginzburg, I. P.
1986-01-01
Two semiempirical theories which provide a basis for determining the turbulent friction and heat exchange near a wall are presented: (1) the Prandtl-Karman theory, and (2) the theory utilizing an equation for the energy of turbulent pulsations. A comparison is made between exact numerical methods and approximate integral methods for computing the turbulent boundary layers in the presence of pressure, blowing, or suction gradients. Using the turbulent flow around a plate as an example, it is shown that, when computing turbulent flows with external turbulence, it is preferable to construct a turbulence model based on the equation for energy of turbulent pulsations.
Unstructured grid methods for compressible flows
NASA Technical Reports Server (NTRS)
Morgan, K.; Peraire, J.; Peiro, J.
1992-01-01
The implementation of the finite element method on unstructured triangular grids is described and the development of centered finite element schemes for the solution of the compressible Euler equation on general triangular and tetrahedral grids is discussed. Explicit and implicit Lax-Wendroff type methods and a method based upon the use of explicit multistep timestepping are considered. In the latter case, the convergence behavior of the method is accelerated by the incorporation of a fully unstructured multigrid procedure. The advancing front method for generating unstructured grids of triangles and tetrahedra is described and the application of adaptive mesh techniques to both steady and transient flow analysis is illustrated.
Method for identifying anomalous terrestrial heat flows
Del Grande, Nancy Kerr
1977-01-25
A method for locating and mapping the magnitude and extent of terrestrial heat-flow anomalies from 5 to 50 times average with a tenfold improved sensitivity over orthodox applications of aerial temperature-sensing surveys as used for geothermal reconnaissance. The method remotely senses surface temperature anomalies such as occur from geothermal resources or oxidizing ore bodies by: measuring the spectral, spatial, statistical, thermal, and temporal features characterizing infrared radiation emitted by natural terrestrial surfaces; deriving from these measurements the true surface temperature with uncertainties as small as 0.05 to 0.5 K; removing effects related to natural temperature variations of topographic, hydrologic, or meteoric origin, the surface composition, detector noise, and atmospheric conditions; factoring out the ambient normal-surface temperature for non-thermally enhanced areas surveyed under otherwise identical environmental conditions; distinguishing significant residual temperature enhancements characteristic of anomalous heat flows and mapping the extent and magnitude of anomalous heat flows where they occur.
Hydraulic flow visualization method and apparatus
Karidis, P.G.
1984-01-01
An apparatus and method for visualizing liquid flow. Pulses of gas bubbles are introduced into a liquid flow stream and a strobe light is operated at a frequency related to the frequency of the gas pulses to shine on the bubbles as they pass through the liquid stream. The gas pulses pass through a probe body having a valve element, and a reciprocating valve stem passes through the probe body to operate the valve element. A stem actuating device comprises a slidable reciprocating member, operated by a crank arm. The actuated member is adjustable to adjust the amount of the valve opening during each pulse.
[Rapid detection of extended-spectrum beta-lactamases by flow cytometry method].
Duyan, Serhat; Kılıç, Abdullah; Yılmaz, Soner; Ardıç, Nurittin
2015-10-01
Extended-spectrum beta-lactamases (ESBL), produced by Enterobacteriaceae members are enzymes that especially cause a resistance to cephalosporin group antibiotics commonly used in clinics. Early and rapid detection of ESBL production is crucial for antimicrobial treatment and infection control; however the methods used for this purpose are time consuming (24 to 48 hours). The aim of this study was to determine a flow cytometry based-test which provides to detect ESBL producing bacteria in a short time. A total of 38 ESBL-producing (29 Escherichia coli, 9 Klebsiella pneumoniae) and 10 non-producing (5 E.coli, 5 K.pneumoniae) Enterobacteriaceae strains isolated between 2012 and 2013 were included in this study. The identification and antibiotic susceptibility tests of the isolates were performed by using Phoenix(TM) 100 automated system (Becton Dickinson, USA). The presence of bla(TEM), bla(SHV), bla(CTX-M1), bla(CTX-M2) and bla(CTX-M9) genes were investigated in ESBL positive isolates via polymerase chain reaction method. At least one of the ESBL genes were detected in 36 out of 38 isolates and no genes were detected in two E.coli isolates. In flow cytometric method, the percentages of death cells exposed to cephalosporin [(ceftazidime (CAZ) or cefotaxime (CTX)] and clavulanic acid (CLA) combination, were compared with death cells exposed only to cephalosporin (CAZ or CTX). CLA index values (CAZ-CLA and CTX-CLA indices) were obtained for CTX and CAZ. Index values which was higher than 1.5 just for one cephalosporin were accepted as GSBL positive. The mean index values for CTX-CLA in ESBL positive strains according to their genotypic characteristics were between 1.14 and 7.22, while those values for CAZ-CLA were between 0.85 and 5.6. When the two groups of 38 ESBL positive and 10 ESBL negative strains were evaluated, statistically significant difference was detected for both CAZ-CLA and CTX-CLA indices (p< 0.005). CTX-CLA indices (p= 0.001) shown a better
Flow coating apparatus and method of coating
Hanumanthu, Ramasubrahmaniam; Neyman, Patrick; MacDonald, Niles; Brophy, Brenor; Kopczynski, Kevin; Nair, Wood
2014-03-11
Disclosed is a flow coating apparatus, comprising a slot that can dispense a coating material in an approximately uniform manner along a distribution blade that increases uniformity by means of surface tension and transfers the uniform flow of coating material onto an inclined substrate such as for example glass, solar panels, windows or part of an electronic display. Also disclosed is a method of flow coating a substrate using the apparatus such that the substrate is positioned correctly relative to the distribution blade, a pre-wetting step is completed where both the blade and substrate are completed wetted with a pre-wet solution prior to dispensing of the coating material onto the distribution blade from the slot and hence onto the substrate. Thereafter the substrate is removed from the distribution blade and allowed to dry, thereby forming a coating.
Method of fabricating a flow device
Hale, Robert L.
1978-01-01
This invention is a novel method for fabricating leak-tight tubular articles which have an interior flow channel whose contour must conform very closely with design specifications but which are composed of metal which tends to warp if welded. The method comprises designing two longitudinal half-sections of the article, the half-sections being contoured internally to cooperatively form the desired flow passageway. Each half-section is designed with a pair of opposed side flanges extending between the end flanges and integral therewith. The half-sections are positioned with their various flanges in confronting relation and with elongated metal gaskets extending between the confronting flanges for the length of the array. The gaskets are a deformable metal which is fusion-weldable to the end flanges. The mating side flanges are joined mechanically to deform the gaskets and provide a longitudinally sealed assembly. The portions of the end flanges contiguous with the ends of the gaskets then are welded to provide localized end welds which incorporate ends of the gaskets, thus transversely sealing the assembly. This method of fabrication provides leak-tight articles having the desired precisely contoured flow channels, whereas various conventional methods have been found unsatisfactory.
Lattice Boltzmann method and channel flow
NASA Astrophysics Data System (ADS)
Stensholt, Sigvat; Mongstad Hope, Sigmund
2016-07-01
Lattice Boltzmann methods are presented at an introductory level with a focus on fairly simple simulations that can be used to test and illustrate the model’s capabilities. Two scenarios are presented. The first is a simple laminar flow in a straight channel driven by a pressure gradient (Poiseuille flow). The second is a more complex, including a wedge where Moffatt vortices may be induced if the wedge is deep enough. Simulations of the Poiseuille flow scenario accurately capture the theoretical velocity profile. The experiment shows the location of the fluid-wall boundary and the effects viscosity has on the velocity and convergence time. The numerical capabilities of the lattice Boltzmann model are tested further by simulating the more complex Moffatt vortex scenario. The method reproduces with high accuracy the theoretical predction that Moffat vortices will not form in a wedge if the vertex angle exceeds 146°. Practical issues limitations of the lattice Boltzmann method are discussed. In particular the accuracy of the bounce-back boundary condition is first order dependent on the grid resolution.
Sawada, Takeshi; Watanabe, Masaru; Fujimura, Yuu; Yagishita, Shigehiro; Shimoyama, Tatsu; Maeda, Yoshiharu; Kanda, Shintaro; Yunokawa, Mayu; Tamura, Kenji; Tamura, Tomohide; Minami, Hironobu; Koh, Yasuhiro; Koizumi, Fumiaki
2016-03-01
Methods for the enumeration and molecular characterization of circulating tumor cells (CTC) have been actively investigated. However, such methods are still technically challenging. We have developed a novel epithelial cell adhesion molecule independent CTC enumeration system integrated with a sorting system using a microfluidics chip. We compared the number of CTC detected using our system with those detected using the CellSearch system in 46 patients with various cancers. We also evaluated epidermal growth factor receptor (EGFR) and PIK3CA mutations of captured CTC in a study of 4 lung cancer and 4 breast cancer patients. The percentage of samples with detected CTC was significantly higher with our system (65.2%) than with CellSearch (28.3%). The number of detected CTC per patient using our system was statistically higher than that using CellSearch (median 5, 0; P = 0.000172, Wilcoxon test). In the mutation analysis study, the number of detected CTC per patient was low (median for lung, 4.5; median for breast, 5.5); however, it was easy to detect EGFR and PIK3CA mutations in the CTC of 2 lung and 1 breast cancer patient, respectively, using a commercially available kit. Our system is more sensitive than CellSearch in CTC enumeration of various cancers and is also capable of detecting EGFR and PIK3CA mutations in the CTC of lung and breast cancer patients, respectively. PMID:26708016
Sawada, Takeshi; Watanabe, Masaru; Fujimura, Yuu; Yagishita, Shigehiro; Shimoyama, Tatsu; Maeda, Yoshiharu; Kanda, Shintaro; Yunokawa, Mayu; Tamura, Kenji; Tamura, Tomohide; Minami, Hironobu; Koh, Yasuhiro; Koizumi, Fumiaki
2016-03-01
Methods for the enumeration and molecular characterization of circulating tumor cells (CTC) have been actively investigated. However, such methods are still technically challenging. We have developed a novel epithelial cell adhesion molecule independent CTC enumeration system integrated with a sorting system using a microfluidics chip. We compared the number of CTC detected using our system with those detected using the CellSearch system in 46 patients with various cancers. We also evaluated epidermal growth factor receptor (EGFR) and PIK3CA mutations of captured CTC in a study of 4 lung cancer and 4 breast cancer patients. The percentage of samples with detected CTC was significantly higher with our system (65.2%) than with CellSearch (28.3%). The number of detected CTC per patient using our system was statistically higher than that using CellSearch (median 5, 0; P = 0.000172, Wilcoxon test). In the mutation analysis study, the number of detected CTC per patient was low (median for lung, 4.5; median for breast, 5.5); however, it was easy to detect EGFR and PIK3CA mutations in the CTC of 2 lung and 1 breast cancer patient, respectively, using a commercially available kit. Our system is more sensitive than CellSearch in CTC enumeration of various cancers and is also capable of detecting EGFR and PIK3CA mutations in the CTC of lung and breast cancer patients, respectively.
Flow cytometric detection method for DNA samples
Nasarabadi, Shanavaz; Langlois, Richard G.; Venkateswaran, Kodumudi S.
2006-08-01
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.
Flow cytometric detection method for DNA samples
Nasarabadi,Shanavaz; Langlois, Richard G.; Venkateswaran, Kodumudi S.
2011-07-05
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.
Cantera, Jason L; Chen, Wilfred; Yates, Marylynn V
2010-01-01
The rapid and effective detection of virus infection is critical for clinical management and prevention of disease spread during an outbreak. Several methods have been developed for this purpose, of which classical serological and viral nucleic acid detection are the most common. We describe an alternative approach that utilizes engineered cells expressing fluorescent proteins undergoing fluorescence resonance energy transfer (FRET) upon cleavage by the viral 2A protease (2A(pro)) as an indication of infection. Quantification of the infectious-virus titers was resolved by using flow cytometry, and utility was demonstrated for the detection of poliovirus 1 (PV1) infection. Engineered buffalo green monkey kidney (BGMK) cells expressing the cyan fluorescent protein (CFP)-yellow fluorescent protein (YFP) substrate linked by a cleavage recognition site for PV1 2A(pro) were infected with different titers of PV1. After incubation at various time points, cells were harvested, washed, and subjected to flow cytometry analysis. The number of infected cells was determined by counting the number of cells with an increased CFP-to-YFP ratio. As early as 5 h postinfection, a significant number of infected cells (3%) was detected by flow cytometry, and cells infected with only 1 PFU were detected after 12 h postinfection. When applied to an environmental water sample spiked with PV1, the flow cytometry-based assay provided a level of sensitivity similar to that of the plaque assay for detecting and quantifying infectious virus particles. This approach, therefore, is more rapid than plaque assays and can be used to detect other viruses that frequently do not form clear plaques on cell cultures.
Immersed boundary methods for viscoelastic particulate flows
NASA Astrophysics Data System (ADS)
Krishnan, Sreenath; Shaqfeh, Eric; Iaccarino, Gianluca
2015-11-01
Viscoelastic particulate suspensions play key roles in many energy applications. Our goal is to develop a simulation-based tool for engineering such suspensions. This study is concerned with fully resolved simulations, wherein all flow scales associated with the particle motion are resolved. The present effort is based on Immersed Boundary methods, in which the domain grids do not conform to particle geometry. In this approach, the conservation of momentum equations, which include both Newtonian and non-Newtonian stresses, are solved over the entire domain including the region occupied by the particles. The particles are defined on a separate Lagrangian mesh that is free to move over an underlying Eulerian grid. The development of an immersed boundary forcing technique for moving bodies within an unstructured-mesh, massively parallel, non-Newtonian flow solver is thus developed and described. The presentation will focus on the numerical algorithm and measures taken to enable efficient parallelization and transfer of information between the underlying fluid grid and the particle mesh. Several validation test cases will be presented including sedimentation under orthogonal shear - a key flow in drilling muds and fracking fluids.
A Filtering Method For Gravitationally Stratified Flows
Gatti-Bono, Caroline; Colella, Phillip
2005-04-25
Gravity waves arise in gravitationally stratified compressible flows at low Mach and Froude numbers. These waves can have a negligible influence on the overall dynamics of the fluid but, for numerical methods where the acoustic waves are treated implicitly, they impose a significant restriction on the time step. A way to alleviate this restriction is to filter out the modes corresponding to the fastest gravity waves so that a larger time step can be used. This paper presents a filtering strategy of the fully compressible equations based on normal mode analysis that is used throughout the simulation to compute the fast dynamics and that is able to damp only fast gravity modes.
Transonic Flow Computations Using Nonlinear Potential Methods
NASA Technical Reports Server (NTRS)
Holst, Terry L.; Kwak, Dochan (Technical Monitor)
2000-01-01
This presentation describes the state of transonic flow simulation using nonlinear potential methods for external aerodynamic applications. The presentation begins with a review of the various potential equation forms (with emphasis on the full potential equation) and includes a discussion of pertinent mathematical characteristics and all derivation assumptions. Impact of the derivation assumptions on simulation accuracy, especially with respect to shock wave capture, is discussed. Key characteristics of all numerical algorithm types used for solving nonlinear potential equations, including steady, unsteady, space marching, and design methods, are described. Both spatial discretization and iteration scheme characteristics are examined. Numerical results for various aerodynamic applications are included throughout the presentation to highlight key discussion points. The presentation ends with concluding remarks and recommendations for future work. Overall. nonlinear potential solvers are efficient, highly developed and routinely used in the aerodynamic design environment for cruise conditions. Published by Elsevier Science Ltd. All rights reserved.
Finite area method for nonlinear conical flows
NASA Technical Reports Server (NTRS)
Sritharan, S. S.; Seebass, A. R.
1982-01-01
A fully conservative finite area method for the computation of steady inviscid flow about general conical bodies at incidence is described. The procedure utilizes the potential approximation and implements a body conforming mesh generator. The conical potential is assumed to have its best linear variation inside each mesh cell and a secondary interlocking cell system is used to establish the flux balance required to conserve mass. In the supersonic regions the scheme is desymmetrized by adding appropriate artificial viscosity in conservation form. The algorithm is nearly an order of a magnitude faster than present Euler methods and predicts known results accurately and qualitative features such as nodal point lift off correctly. Results are compared with those of other investigations.
Flow “Fine” Synthesis: High Yielding and Selective Organic Synthesis by Flow Methods
2015-01-01
Abstract The concept of flow “fine” synthesis, that is, high yielding and selective organic synthesis by flow methods, is described. Some examples of flow “fine” synthesis of natural products and APIs are discussed. Flow methods have several advantages over batch methods in terms of environmental compatibility, efficiency, and safety. However, synthesis by flow methods is more difficult than synthesis by batch methods. Indeed, it has been considered that synthesis by flow methods can be applicable for the production of simple gasses but that it is difficult to apply to the synthesis of complex molecules such as natural products and APIs. Therefore, organic synthesis of such complex molecules has been conducted by batch methods. On the other hand, syntheses and reactions that attain high yields and high selectivities by flow methods are increasingly reported. Flow methods are leading candidates for the next generation of manufacturing methods that can mitigate environmental concerns toward sustainable society. PMID:26337828
Flow "Fine" Synthesis: High Yielding and Selective Organic Synthesis by Flow Methods.
Kobayashi, Shū
2016-02-18
The concept of flow "fine" synthesis, that is, high yielding and selective organic synthesis by flow methods, is described. Some examples of flow "fine" synthesis of natural products and APIs are discussed. Flow methods have several advantages over batch methods in terms of environmental compatibility, efficiency, and safety. However, synthesis by flow methods is more difficult than synthesis by batch methods. Indeed, it has been considered that synthesis by flow methods can be applicable for the production of simple gasses but that it is difficult to apply to the synthesis of complex molecules such as natural products and APIs. Therefore, organic synthesis of such complex molecules has been conducted by batch methods. On the other hand, syntheses and reactions that attain high yields and high selectivities by flow methods are increasingly reported. Flow methods are leading candidates for the next generation of manufacturing methods that can mitigate environmental concerns toward sustainable society.
Nuclear reactor flow control method and apparatus
Church, John P.
1993-01-01
Method and apparatus for improving coolant flow in a nuclear reactor during accident as well as nominal conditions. The reactor has a plurality of fuel elements in sleeves and a plenum above the fuel and through which the sleeves penetrate. Holes are provided in the sleeve so that coolant from the plenum can enter the sleeve and cool the fuel. The number and size of the holes are varied from sleeve to sleeve with the number and size of holes being greater for sleeves toward the center of the core and less for sleeves toward the periphery of the core. Preferably the holes are all the same diameter and arranged in rows and columns, the rows starting from the bottom of every sleeve and fewer rows in peripheral sleeves and more rows in the central sleeves.
Method and Apparatus for Measuring Fluid Flow
NASA Technical Reports Server (NTRS)
Arndt, G. Dickey (Inventor); Nguyen, Thanh X. (Inventor); Carl, James R. (Inventor)
1997-01-01
Method and apparatus for making measurements on fluids related to their complex permeability are disclosed. A microwave probe is provided for exposure to the fluids. The probe can be non-intrusive or can also be positioned at the location where measurements are to be made. The impedance of the probe is determined. in part. by the complex dielectric constant of the fluids at the probe. A radio frequency signal is transmitted to the probe and the reflected signal is phase and amplitude detected at a rapid rate for the purpose of identifying the fluids. Multiple probes may be selectively positioned to monitor the behavior of the fluids including their flow rate. Fluids may be identified as between two or more different fluids as well as multiple phases of the same fluid based on differences between their complex permittivities.
Calculation of transonic flows using an extended integral equation method
NASA Technical Reports Server (NTRS)
Nixon, D.
1976-01-01
An extended integral equation method for transonic flows is developed. In the extended integral equation method velocities in the flow field are calculated in addition to values on the aerofoil surface, in contrast with the less accurate 'standard' integral equation method in which only surface velocities are calculated. The results obtained for aerofoils in subcritical flow and in supercritical flow when shock waves are present compare satisfactorily with the results of recent finite difference methods.
Computational methods for internal flows with emphasis on turbomachinery
NASA Technical Reports Server (NTRS)
Mcnally, W. D.; Sockol, P. M.
1981-01-01
Current computational methods for analyzing flows in turbomachinery and other related internal propulsion components are presented. The methods are divided into two classes. The inviscid methods deal specifically with turbomachinery applications. Viscous methods, deal with generalized duct flows as well as flows in turbomachinery passages. Inviscid methods are categorized into the potential, stream function, and Euler aproaches. Viscous methods are treated in terms of parabolic, partially parabolic, and elliptic procedures. Various grids used in association with these procedures are also discussed.
Method and apparatus for measuring flow velocity using matched filters
Raptis, A.C.
1983-09-06
An apparatus and method for measuring the flow velocities of individual phase flow components of a multiphase flow utilizes matched filters. Signals arising from flow noise disturbance are extracted from the flow, at upstream and downstream locations. The signals are processed through pairs of matched filters which are matched to the flow disturbance frequency characteristics of the phase flow component to be measured. The processed signals are then cross-correlated to determine the transit delay time of the phase flow component between sensing positions. 8 figs.
Method and apparatus for measuring flow velocity using matched filters
Raptis, Apostolos C.
1983-01-01
An apparatus and method for measuring the flow velocities of individual phase flow components of a multiphase flow utilizes matched filters. Signals arising from flow noise disturbance are extracted from the flow, at upstream and downstream locations. The signals are processed through pairs of matched filters which are matched to the flow disturbance frequency characteristics of the phase flow component to be measured. The processed signals are then cross-correlated to determine the transit delay time of the phase flow component between sensing positions.
Microfluidic devices and methods for integrated flow cytometry
Srivastava, Nimisha; Singh, Anup K.
2011-08-16
Microfluidic devices and methods for flow cytometry are described. In described examples, various sample handling and preparation steps may be carried out within a same microfluidic device as flow cytometry steps. A combination of imaging and flow cytometry is described. In some examples, spiral microchannels serve as incubation chambers. Examples of automated sample handling and flow cytometry are described.
Method and Apparatus for Measuring Fluid Flow
NASA Technical Reports Server (NTRS)
Arndt, G. Dickey (Inventor); Nguyen, Than X. (Inventor); Carl, James R. (Inventor)
1995-01-01
The invention is a method and apparatus for monitoring the presence, concentration, and the movement of fluids. It is based on utilizing electromagnetic measurements of the complex permittivity of the fluids for detecting and monitoring the fluid. More particularly the apparatus uses one or more microwave probes which are placed at the locations where the measurements are to be made. A radio frequency signal is transmitted to the probe and the reflected signal is phase and amplitude detected at a rapid rate for the purpose of identifying the fluids, based on their dielectric constant at the probe. The apparatus can be used for multiple purposes including measures of flow rates, turbulence, dispersion, fluid identification, and changes in flow conditions of multiple fluids or multiple states of a single fluid in a flowline or a holding container. The apparatus includes a probe consisting of two electrical conductors separated by an insulator. A radio frequency signal is communicated to the probe and is reflected back from the portion of the probe exposed to the fluid. The radio frequency signal also provides a reference signal. An oscillator generates a second signal which combined with each of the reference signal and the reflected signal to produce signals of lower frequencies to facilitate filtering and amplifying those signals. The two signals are then mixed in a detector to produce an output signal that is representative of the phase and amplitude change caused by the reflection of the signal at the probe exposed to the fluid. The detector may be a dual phase detector that provides two such output signals that are in phase quadrature. A phase shifter may be provided for selectively changing the phase of the reference signal to improve the sensitivity of at least one of the output signals for more accurate readings and/or for calibration purposes. The two outputs that are in quadrature with respect to each other may be simultaneously monitored to account for
Boundary integral methods for unsaturated flow
Martinez, M.J.; McTigue, D.F.
1990-12-31
Many large simulations may be required to assess the performance of Yucca Mountain as a possible site for the nations first high level nuclear waste repository. A boundary integral equation method (BIEM) is described for numerical analysis of quasilinear steady unsaturated flow in homogeneous material. The applicability of the exponential model for the dependence of hydraulic conductivity on pressure head is discussed briefly. This constitutive assumption is at the heart of the quasilinear transformation. Materials which display a wide distribution in pore-size are described reasonably well by the exponential. For materials with a narrow range in pore-size, the exponential is suitable over more limited ranges in pressure head. The numerical implementation of the BIEM is used to investigate the infiltration from a strip source to a water table. The net infiltration of moisture into a finite-depth layer is well-described by results for a semi-infinite layer if {alpha}D > 4, where {alpha} is the sorptive number and D is the depth to the water table. the distribution of moisture exhibits a similar dependence on {alpha}D. 11 refs., 4 figs.,
On methods of estimating cosmological bulk flows
NASA Astrophysics Data System (ADS)
Nusser, Adi
2016-01-01
We explore similarities and differences between several estimators of the cosmological bulk flow, B, from the observed radial peculiar velocities of galaxies. A distinction is made between two theoretical definitions of B as a dipole moment of the velocity field weighted by a radial window function. One definition involves the three-dimensional (3D) peculiar velocity, while the other is based on its radial component alone. Different methods attempt at inferring B for either of these definitions which coincide only for the case of a velocity field which is constant in space. We focus on the Wiener Filtering (WF) and the Constrained Minimum Variance (CMV) methodologies. Both methodologies require a prior expressed in terms of the radial velocity correlation function. Hoffman et al. compute B in Top-Hat windows from a WF realization of the 3D peculiar velocity field. Feldman et al. infer B directly from the observed velocities for the second definition of B. The WF methodology could easily be adapted to the second definition, in which case it will be equivalent to the CMV with the exception of the imposed constraint. For a prior with vanishing correlations or very noisy data, CMV reproduces the standard Maximum Likelihood estimation for B of the entire sample independent of the radial weighting function. Therefore, this estimator is likely more susceptible to observational biases that could be present in measurements of distant galaxies. Finally, two additional estimators are proposed.
PDF methods for turbulent reactive flows
NASA Technical Reports Server (NTRS)
Hsu, Andrew T.
1995-01-01
Viewgraphs are presented on computation of turbulent combustion, governing equations, closure problem, PDF modeling of turbulent reactive flows, validation cases, current projects, and collaboration with industry and technology transfer.
Field methods for measuring concentrated flow erosion
NASA Astrophysics Data System (ADS)
Castillo, C.; Pérez, R.; James, M. R.; Quinton, J. N.; Taguas, E. V.; Gómez, J. A.
2012-04-01
techniques (3D) for measuring erosion from concentrated flow (pole, laser profilemeter, photo-reconstruction and terrestrial LiDAR) The comparison between two- and three-dimensional methods has showed the superiority of the 3D techniques for obtaining accurate cross sectional data. The results from commonly-used 2D methods can be subject to systematic errors in areal cross section that exceed magnitudes of 10 % on average. In particular, the pole simplified method has showed a clear tendency to understimate areas. Laser profilemeter results show that further research on calibrating optical devices for a variety of soil conditions must be carried out to improve its performance. For volume estimations, photo-reconstruction results provided an excellent approximation to terrestrial laser data and demonstrate that this new remote sensing technique has a promising application field in soil erosion studies. 2D approaches involved important errors even over short measurement distances. However, as well as accuracy, the cost and time requirements of a technique must be considered.
Rapid flow cytometric measurement of protein inclusions and nuclear trafficking
Whiten, D. R.; San Gil, R.; McAlary, L.; Yerbury, J. J.; Ecroyd, H.; Wilson, M. R.
2016-01-01
Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules. PMID:27516358
Rapid flow cytometric measurement of protein inclusions and nuclear trafficking.
Whiten, D R; San Gil, R; McAlary, L; Yerbury, J J; Ecroyd, H; Wilson, M R
2016-01-01
Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules. PMID:27516358
Moving and adaptive grid methods for compressible flows
NASA Technical Reports Server (NTRS)
Trepanier, Jean-Yves; Camarero, Ricardo
1995-01-01
This paper describes adaptive grid methods developed specifically for compressible flow computations. The basic flow solver is a finite-volume implementation of Roe's flux difference splitting scheme or arbitrarily moving unstructured triangular meshes. The grid adaptation is performed according to geometric and flow requirements. Some results are included to illustrate the potential of the methodology.
Method and apparatus for monitoring two-phase flow. [PWR
Sheppard, J.D.; Tong, L.S.
1975-12-19
A method and apparatus for monitoring two-phase flow is provided that is particularly related to the monitoring of transient two-phase (liquid-vapor) flow rates such as may occur during a pressurized water reactor core blow-down. The present invention essentially comprises the use of flanged wire screens or similar devices, such as perforated plates, to produce certain desirable effects in the flow regime for monitoring purposes. One desirable effect is a measurable and reproducible pressure drop across the screen. The pressure drop can be characterized for various known flow rates and then used to monitor nonhomogeneous flow regimes. Another useful effect of the use of screens or plates in nonhomogeneous flow is that such apparatus tends to create a uniformly dispersed flow regime in the immediate downstream vicinity. This is a desirable effect because it usually increases the accuracy of flow rate measurements determined by conventional methods.
Noninvasive method of estimating human newborn regional cerebral blood flow
Younkin, D.P.; Reivich, M.; Jaggi, J.; Obrist, W.; Delivoria-Papadopoulos, M.
1982-12-01
A noninvasive method of estimating regional cerebral blood flow (rCBF) in premature and full-term babies has been developed. Based on a modification of the /sup 133/Xe inhalation rCBF technique, this method uses eight extracranial NaI scintillation detectors and an i.v. bolus injection of /sup 133/Xe (approximately 0.5 mCi/kg). Arterial xenon concentration was estimated with an external chest detector. Cerebral blood flow was measured in 15 healthy, neurologically normal premature infants. Using Obrist's method of two-compartment analysis, normal values were calculated for flow in both compartments, relative weight and fractional flow in the first compartment (gray matter), initial slope of gray matter blood flow, mean cerebral blood flow, and initial slope index of mean cerebral blood flow. The application of this technique to newborns, its relative advantages, and its potential uses are discussed.
A method of determining combustion gas flow
NASA Technical Reports Server (NTRS)
Bon Tempi, P. J.
1968-01-01
Zirconium oxide coating enables the determination of hot gas flow patterns on liquid rocket injector face and baffle surfaces to indicate modifications that will increase performance and improve combustion stability. The coating withstands combustion temperatures and due to the coarse surface and coloring of the coating, shows the hot gas patterns.
APPROXIMATE MULTIPHASE FLOW MODELING BY CHARACTERISTIC METHODS
The flow of petroleum hydrocarbons, organic solvents and other liquids that are immiscible with water presents the nation with some of the most difficult subsurface remediation problems. One aspect of contaminant transport associated releases of such liquids is the transport as a...
Methods for improved resolution of flow electrophoresis cells
NASA Technical Reports Server (NTRS)
Mccreight, L. R.; Fogal, G. L.
1974-01-01
First method involves remote adjusting of zeta potential. Second approach sandwiches two conducting metal plates between opposite cell walls and thin insulating layer. Third method forces buffer to flow in direction opposite particle streams.
Impulse-based methods for fluid flow
Cortez, R.
1995-05-01
A Lagrangian numerical method based on impulse variables is analyzed. A relation between impulse vectors and vortex dipoles with a prescribed dipole moment is presented. This relation is used to adapt the high-accuracy cutoff functions of vortex methods for use in impulse-based methods. A source of error in the long-time implementation of the impulse method is explained and two techniques for avoiding this error are presented. An application of impulse methods to the motion of a fluid surrounded by an elastic membrane is presented.
Ultrasonic fluid flow measurement method and apparatus
Kronberg, J.W.
1993-10-12
An apparatus for measuring the flow of a fluid in a pipe using ultrasonic waves. The apparatus comprises an ultrasonic generator, a lens for focusing the sound energy produced by the generator, and means for directing the focused energy into the side of the pipe through an opening and in a direction close to parallel to the long axis of the pipe. A cone carries the sound energy to the lens from the generator. Depending on the choice of materials, there may be a quarter-wave, acoustic impedance matching section between the generator and the cone to reduce the reflections of energy at the cone boundary. The lens material has an acoustic impedance similar to that of the cone material but a different sonic velocity so that the lens can converge the sound waves in the fluid. A transition section between the lens and the fluid helps to couple the energy to the fluid and assures it is directed as close to parallel to the fluid flow direction as possible. 3 figures.
Ultrasonic fluid flow measurement method and apparatus
Kronberg, James W.
1993-01-01
An apparatus for measuring the flow of a fluid in a pipe using ultrasonic waves. The apparatus comprises an ultrasonic generator, a lens for focusing the sound energy produced by the generator, and means for directing the focused energy into the side of the pipe through an opening and in a direction close to parallel to the long axis of the pipe. A cone carries the sound energy to the lens from the generator. Depending on the choice of materials, there may be a quarter-wave, acoustic impedance matching section between the generator and the cone to reduce the reflections of energy at the cone boundary. The lens material has an acoustic impedance similar to that of the cone material but a different sonic velocity so that the lens can converge the sound waves in the fluid. A transition section between the lens and the fluid helps to couple the energy to the fluid and assures it is directed as close to parallel to the fluid flow direction as possible.
Lattice Boltzmann methods for viscous fluid flows and for two-phase fluid flows
NASA Astrophysics Data System (ADS)
Inamuro, Takaji
2006-09-01
Lattice Boltzmann methods (LBMs) for viscous fluid flows and for two-phase fluid flows are presented. First, the LBMs for incompressible viscous fluid flows and for temperature fields are described. Then, we derive a lattice kinetic scheme (LKS) which is an improved scheme of the LBM. The LKS does not require any velocity distribution functions and is more stable than the LBMs. In addition, the LBM for two-phase fluid flows is presented. The method can simulate flows with the density ratio up to 1000. Numerical examples of unsteady flows in a three-dimensional porous structure, binary droplet collision and rising bubbles in a square duct are illustrated. It is expected that the LBMs (and LKS) will become promising numerical schemes for simulating complex fluid flows.
Fourier time spectral method for subsonic and transonic flows
NASA Astrophysics Data System (ADS)
Zhan, Lei; Liu, Feng; Papamoschou, Dimitri
2016-06-01
The time accuracy of the exponentially accurate Fourier time spectral method (TSM) is examined and compared with a conventional 2nd-order backward difference formula (BDF) method for periodic unsteady flows. In particular, detailed error analysis based on numerical computations is performed on the accuracy of resolving the local pressure coefficient and global integrated force coefficients for smooth subsonic and non-smooth transonic flows with moving shock waves on a pitching airfoil. For smooth subsonic flows, the Fourier TSM method offers a significant accuracy advantage over the BDF method for the prediction of both the local pressure coefficient and integrated force coefficients. For transonic flows where the motion of the discontinuous shock wave contributes significant higher-order harmonic contents to the local pressure fluctuations, a sufficient number of modes must be included before the Fourier TSM provides an advantage over the BDF method. The Fourier TSM, however, still offers better accuracy than the BDF method for integrated force coefficients even for transonic flows. A problem of non-symmetric solutions for symmetric periodic flows due to the use of odd numbers of intervals is uncovered and analyzed. A frequency-searching method is proposed for problems where the frequency is not known a priori. The method is tested on the vortex shedding problem of the flow over a circular cylinder.
Method and apparatus for coal analysis and flow measurement
Rollwitz, W.L.; King, J.D.
1985-07-23
A flow meter apparatus and method for measuring the flow, composition and heat content of coal is set forth. In the preferred and illustrated embodiment, the free or unpaired electron population of flowing coal is measured by electron magnetic resonance (EMR); the hydrogen nucleii population is measured by nuclear magnetic resonance (NMR). By calibration utilizing a standard specimen for a given type of coal, a profile for various types of coal can be obtained wherein measurement data is converted into an indication of the heat content typically measured in BTU per pound. This enables provision of a volumetric flow meter, a flow meter indicating flow in calorie content. This device enables integration to provide total heat content flow. Other variables describing the coal can be obtained.
Advanced surface paneling method for subsonic and supersonic flow
NASA Technical Reports Server (NTRS)
Erickson, L. L.; Johnson, F. T.; Ehlers, F. E.
1976-01-01
Numerical results illustrating the capabilities of an advanced aerodynamic surface paneling method are presented. The method is applicable to both subsonic and supersonic flow, as represented by linearized potential flow theory. The method is based on linearly varying sources and quadratically varying doublets which are distributed over flat or curved panels. These panels are applied to the true surface geometry of arbitrarily shaped three dimensional aerodynamic configurations.
A Reconstruction Method of Blood Flow Velocity in Left Ventricle Using Color Flow Ultrasound.
Jang, Jaeseong; Ahn, Chi Young; Jeon, Kiwan; Heo, Jung; Lee, DongHak; Joo, Chulmin; Choi, Jung-il; Seo, Jin Keun
2015-01-01
Vortex flow imaging is a relatively new medical imaging method for the dynamic visualization of intracardiac blood flow, a potentially useful index of cardiac dysfunction. A reconstruction method is proposed here to quantify the distribution of blood flow velocity fields inside the left ventricle from color flow images compiled from ultrasound measurements. In this paper, a 2D incompressible Navier-Stokes equation with a mass source term is proposed to utilize the measurable color flow ultrasound data in a plane along with the moving boundary condition. The proposed model reflects out-of-plane blood flows on the imaging plane through the mass source term. The boundary conditions to solve the system of equations are derived from the dimensions of the ventricle extracted from 2D echocardiography data. The performance of the proposed method is evaluated numerically using synthetic flow data acquired from simulating left ventricle flows. The numerical simulations show the feasibility and potential usefulness of the proposed method of reconstructing the intracardiac flow fields. Of particular note is the finding that the mass source term in the proposed model improves the reconstruction performance. PMID:26078773
Chaotic maps, Hamiltonian flows, and Holographic methods.
Curtright, T. L.; Zachos, C. K.; High Energy Physics; Univ. of Miami
2010-01-01
Holographic functional methods are introduced as probes of discrete time-stepped maps that lead to chaotic behavior. The methods provide continuous time interpolation between the time steps, thereby revealing the maps to be quasi-Hamiltonian systems underlain by novel potentials that govern the motion of a perceived point particle. Between turning points, the particle is strictly driven by Hamiltonian dynamics, but at each encounter with a turning point the potential changes abruptly, loosely analogous to the switchbacks on a mountain road. A sequence of successively deepening switchback potentials explains, in physical terms, the frequency cascade and trajectory folding that occur on the particular route to chaos revealed by the logistic map.
Vortical Flow Prediction Using an Adaptive Unstructured Grid Method
NASA Technical Reports Server (NTRS)
Pirzadeh, Shahyar Z.
2003-01-01
A computational fluid dynamics (CFD) method has been employed to compute vortical flows around slender wing/body configurations. The emphasis of the paper is on the effectiveness of an adaptive grid procedure in "capturing" concentrated vortices generated at sharp edges or flow separation lines of lifting surfaces flying at high angles of attack. The method is based on a tetrahedral unstructured grid technology developed at the NASA Langley Research Center. Two steady-state, subsonic, inviscid and Navier-Stokes flow test cases are presented to demonstrate the applicability of the method for solving practical vortical flow problems. The first test case concerns vortex flow over a simple 65 delta wing with different values of leading-edge radius. Although the geometry is quite simple, it poses a challenging problem for computing vortices originating from blunt leading edges. The second case is that of a more complex fighter configuration. The superiority of the adapted solutions in capturing the vortex flow structure over the conventional unadapted results is demonstrated by comparisons with the wind-tunnel experimental data. The study shows that numerical prediction of vortical flows is highly sensitive to the local grid resolution and that the implementation of grid adaptation is essential when applying CFD methods to such complicated flow problems.
Vortical Flow Prediction Using an Adaptive Unstructured Grid Method
NASA Technical Reports Server (NTRS)
Pirzadeh, Shahyar Z.
2001-01-01
A computational fluid dynamics (CFD) method has been employed to compute vortical flows around slender wing/body configurations. The emphasis of the paper is on the effectiveness of an adaptive grid procedure in "capturing" concentrated vortices generated at sharp edges or flow separation lines of lifting surfaces flying at high angles of attack. The method is based on a tetrahedral unstructured grid technology developed at the NASA Langley Research Center. Two steady-state, subsonic, inviscid and Navier-Stokes flow test cases are presented to demonstrate the applicability of the method for solving practical vortical flow problems. The first test case concerns vortex flow over a simple 65deg delta wing with different values of leading-edge bluntness, and the second case is that of a more complex fighter configuration. The superiority of the adapted solutions in capturing the vortex flow structure over the conventional unadapted results is demonstrated by comparisons with the windtunnel experimental data. The study shows that numerical prediction of vortical flows is highly sensitive to the local grid resolution and that the implementation of grid adaptation is essential when applying CFD methods to such complicated flow problems.
Construction of School Timetables by Flow Methods.
ERIC Educational Resources Information Center
de Werra, D.
In this paper, a heuristic algorithm for constructing school timetables is described. The algorithm is based on an exact method that applies to a family of particular timetable problems. The procedure has been used to construct timetables for Swiss schools having about 50 classes, 80 teachers, and 35 weekly periods. Less than five percent of…
PDF methods for combustion in high-speed turbulent flows
NASA Technical Reports Server (NTRS)
Pope, Stephen B.
1995-01-01
This report describes the research performed during the second year of this three-year project. The ultimate objective of the project is extend the applicability of probability density function (pdf) methods from incompressible to compressible turbulent reactive flows. As described in subsequent sections, progress has been made on: (1) formulation and modelling of pdf equations for compressible turbulence, in both homogeneous and inhomogeneous inert flows; and (2) implementation of the compressible model in various flow configurations, namely decaying isotropic turbulence, homogeneous shear flow and plane mixing layer.
The art and science of flow control - case studies using flow visualization methods
NASA Astrophysics Data System (ADS)
Alvi, F. S.; Cattafesta, L. N., III
2010-04-01
Active flow control (AFC) has been the focus of significant research in the last decade. This is mainly due to the potentially substantial benefits it affords. AFC applications range from the subsonic to the supersonic (and beyond) regime for both internal and external flows. These applications are wide and varied, such as controlling flow transition and separation over various external components of the aircraft to active management of separation and flow distortion in engine components and over turbine and compressor blades. High-speed AFC applications include control of flow oscillations in cavity flows, supersonic jet screech, impinging jets, and jet-noise control. In this paper we review some of our recent applications of AFC through a number of case studies that illustrate the typical benefits as well as limitations of present AFC methods. The case studies include subsonic and supersonic canonical flowfields such as separation control over airfoils, control of supersonic cavity flows and impinging jets. In addition, properties of zero-net mass-flux (ZNMF) actuators are also discussed as they represent one of the most widely studied actuators used for AFC. In keeping with the theme of this special issue, the flowfield properties and their response to actuation are examined through the use of various qualitative and quantitative flow visualization methods, such as smoke, shadowgraph, schlieren, planar-laser scattering, and Particle image velocimetry (PIV). The results presented here clearly illustrate the merits of using flow visualization to gain significant insight into the flow and its response to AFC.
Developments in flow visualization methods for flight research
NASA Technical Reports Server (NTRS)
Holmes, Bruce J.; Obara, Clifford J.; Manuel, Gregory S.; Lee, Cynthia C.
1990-01-01
With the introduction of modern airplanes utilizing laminar flow, flow visualization has become an important diagnostic tool in determining aerodynamic characteristics such as surface flow direction and boundary-layer state. A refinement of the sublimating chemical technique has been developed to define both the boundary-layer transition location and the transition mode. In response to the need for flow visualization at subsonic and transonic speeds and altitudes above 20,000 feet, the liquid crystal technique has been developed. A third flow visualization technique that has been used is infrared imaging, which offers non-intrusive testing over a wide range of test conditions. A review of these flow visualization methods and recent flight results is presented for a variety of modern aircraft and flight conditions.
Inconsistency of Species Tree Methods under Gene Flow.
Solís-Lemus, Claudia; Yang, Mengyao; Ané, Cécile
2016-09-01
Coalescent-based methods are now broadly used to infer evolutionary relationships between groups of organisms under the assumption that incomplete lineage sorting (ILS) is the only source of gene tree discordance. Many of these methods are known to consistently estimate the species tree when all their assumptions are met. Nonetheless, little work has been done to test the robustness of such methods to violations of their assumptions. Here, we study the performance of two of the most efficient coalescent-based methods, ASTRAL and NJst, in the presence of gene flow. Gene flow violates the assumption that ILS is the sole source of gene tree conflict. We find anomalous gene trees on three-taxon rooted trees and on four-taxon unrooted trees. These anomalous trees do not exist under ILS only, but appear because of gene flow. Our simulations show that species tree methods (and concatenation) may reconstruct the wrong evolutionary history, even from a very large number of well-reconstructed gene trees. In other words, species tree methods can be inconsistent under gene flow. Our results underline the need for methods like PhyloNet, to account simultaneously for ILS and gene flow in a unified framework. Although much slower, PhyloNet had better accuracy and remained consistent at high levels of gene flow. PMID:27151419
A flux monitoring method for easy and accurate flow rate measurement in pressure-driven flows.
Siria, Alessandro; Biance, Anne-Laure; Ybert, Christophe; Bocquet, Lydéric
2012-03-01
We propose a low-cost and versatile method to measure flow rate in microfluidic channels under pressure-driven flows, thereby providing a simple characterization of the hydrodynamic permeability of the system. The technique is inspired by the current monitoring method usually employed to characterize electro-osmotic flows, and makes use of the measurement of the time-dependent electric resistance inside the channel associated with a moving salt front. We have successfully tested the method in a micrometer-size channel, as well as in a complex microfluidic channel with a varying cross-section, demonstrating its ability in detecting internal shape variations.
DEMONSTRATION BULLETIN: COLLOID POLISHING FILTER METHOD - FILTER FLOW TECHNOLOGY, INC.
The Filter Flow Technology, Inc. (FFT) Colloid Polishing Filter Method (CPFM) was tested as a transportable, trailer mounted, system that uses sorption and chemical complexing phenomena to remove heavy metals and nontritium radionuclides from water. Contaminated waters can be pro...
Pressure algorithm for elliptic flow calculations with the PDF method
NASA Technical Reports Server (NTRS)
Anand, M. S.; Pope, S. B.; Mongia, H. C.
1991-01-01
An algorithm to determine the mean pressure field for elliptic flow calculations with the probability density function (PDF) method is developed and applied. The PDF method is a most promising approach for the computation of turbulent reacting flows. Previous computations of elliptic flows with the method were in conjunction with conventional finite volume based calculations that provided the mean pressure field. The algorithm developed and described here permits the mean pressure field to be determined within the PDF calculations. The PDF method incorporating the pressure algorithm is applied to the flow past a backward-facing step. The results are in good agreement with data for the reattachment length, mean velocities, and turbulence quantities including triple correlations.
Packet flow monitoring tool and method
Thiede, David R [Richland, WA
2009-07-14
A system and method for converting packet streams into session summaries. Session summaries are a group of packets each having a common source and destination internet protocol (IP) address, and, if present in the packets, common ports. The system first captures packets from a transport layer of a network of computer systems, then decodes the packets captured to determine the destination IP address and the source IP address. The system then identifies packets having common destination IP addresses and source IP addresses, then writes the decoded packets to an allocated memory structure as session summaries in a queue.
Characterization of peak flow events with local singularity method
NASA Astrophysics Data System (ADS)
Cheng, Q.; Li, L.; Wang, L.
2009-07-01
Three methods, return period, power-law frequency plot (concentration-area) and local singularity index, are introduced in the paper for characterizing peak flow events from river flow data for the past 100 years from 1900 to 2000 recorded at 25 selected gauging stations on rivers in the Oak Ridges Moraine (ORM) area, Canada. First a traditional method, return period, was applied to the maximum annual river flow data. Whereas the Pearson III distribution generally fits the values, a power-law frequency plot (C-A) on the basis of self-similarity principle provides an effective mean for distinguishing "extremely" large flow events from the regular flow events. While the latter show a power-law distribution, about 10 large flow events manifest departure from the power-law distribution and these flow events can be classified into a separate group most of which are related to flood events. It is shown that the relation between the average water releases over a time period after flow peak and the time duration may follow a power-law distribution. The exponent of the power-law or singularity index estimated from this power-law relation may be used to characterize non-linearity of peak flow recessions. Viewing large peak flow events or floods as singular processes can anticipate the application of power-law models not only for characterizing the frequency distribution of peak flow events, for example, power-law relation between the number and size of floods, but also for describing local singularity of processes such as power-law relation between the amount of water released versus releasing time. With the introduction and validation of singularity of peak flow events, alternative power-law models can be used to depict the recession property as well as other types of non-linear properties.
Semiempirical method of determining flow coefficients for pitot rake mass flow rate measurements
NASA Technical Reports Server (NTRS)
Trefny, C. J.
1985-01-01
Flow coefficients applicable to area-weighted pitot rake mass flow rate measurements are presented for fully developed, turbulent flow in an annulus. A turbulent velocity profile is generated semiempirically for a given annulus hub-to-tip radius ratio and integrated numerically to determine the ideal mass flow rate. The calculated velocities at each probe location are then summed, and the flow rate as indicated by the rake is obtained. The flow coefficient to be used with the particular rake geometry is subsequently obtained by dividing the ideal flow rate by the rake-indicated flow rate. Flow coefficients ranged from 0.903 for one probe placed at a radius dividing two equal areas to 0.984 for a 10-probe area-weighted rake. Flow coefficients were not a strong function of annulus hub-to-tip radius ratio for rakes with three or more probes. The semiempirical method used to generate the turbulent velocity profiles is described in detail.
A Fast Estimation Method of Railway Passengers' Flow
NASA Astrophysics Data System (ADS)
Nagasaki, Yusaku; Asuka, Masashi; Komaya, Kiyotoshi
To evaluate a train schedule from the viewpoint of passengers' convenience, it is important to know each passenger's choice of trains and transfer stations to arrive at his/her destination. Because of difficulties of measuring such passengers' behavior, estimation methods of railway passengers' flow are proposed to execute such an evaluation. However, a train schedule planning system equipped with those methods is not practical due to necessity of much time to complete the estimation. In this article, the authors propose a fast passengers' flow estimation method that employs features of passengers' flow graph using preparative search based on each train's arrival time at each station. And the authors show the results of passengers' flow estimation applied on a railway in an urban area.
NASA Technical Reports Server (NTRS)
Hamrick, Joseph T; Ginsburg, Ambrose; Osborn, Walter M
1952-01-01
A method is presented for analysis of the compressible flow between the hub and the shroud of mixed-flow impellers of arbitrary design. Axial symmetry was assumed, but the forces in the meridional (hub to shroud) plane, which are derived from tangential pressure gradients, were taken into account. The method was applied to an experimental mixed-flow impeller. The analysis of the flow in the meridional plane of the impeller showed that the rotational forces, the blade curvature, and the hub-shroud profile can introduce severe velocity gradients along the hub and the shroud surfaces. Choked flow at the impeller inlet as determined by the analysis was verified by experimental results.
On the no-field method for void time determination in flow field-flow fractionation.
Martin, Michel; Hoyos, Mauricio
2011-07-01
Elution time measurements of colloidal particles injected in a symmetrical flow field-flow fractionation (flow FFF) system when the inlet and outlet cross-flow connections are closed have been performed. This no-field method has been proposed earlier for void time (and void volume) determination in flow FFF Giddings et al. (1977). The elution times observed were much larger than expected on the basis of the channel geometrical volume and the flow rate. In order to explain these discrepancies, a flow model allowing the carrier liquid to flow through the porous walls toward the reservoirs located behind the porous elements and along these reservoirs was developed. The ratio between the observed elution time and expected one is found to depend only on a parameter which is a function of the effective permeability and thickness of the porous elements and of the channel thickness and length. The permeabilities of the frits used in the system were measured. Their values lead to predicted elution times in reasonable agreement with experimental ones, taking into account likely membrane protrusion inside the channel on system assembly. They comfort the basic feature of the flow model, in the no-field case. The carrier liquid mostly bypasses the channel to flow along the system mainly in the reservoir. It flows through the porous walls toward the reservoirs near channel inlet and again through the porous walls from the reservoirs to the channel near channel outlet before exiting the system. In order to estimate the extent of this bypassing process, it is desirable that the hydrodynamic characteristics of the permeable elements (permeability and thickness) are provided by flow FFF manufacturers. The model applies to symmetrical as well as asymmetrical flow FFF systems. PMID:21256498
Method, apparatus and system for controlling fluid flow
McMurtrey, Ryan D.; Ginosar, Daniel M.; Burch, Joesph V.
2007-10-30
A system, apparatus and method of controlling the flow of a fluid are provided. In accordance with one embodiment of the present invention, a flow control device includes a valve having a flow path defined therethrough and a valve seat in communication with the flow path with a valve stem disposed in the valve seat. The valve stem and valve seat are cooperatively configured to cause mutual relative linear displacement thereof in response to rotation of the valve stem. A gear member is coupled with the rotary stem and a linear positioning member includes a portion which complementarily engages the gear member. Upon displacement of the linear positioning member along a first axis, the gear member and rotary valve stem are rotated about a second axis and the valve stem and valve seat are mutually linearly displaced to alter the flow of fluid through the valve.
A study of methods to estimate debris flow velocity
Prochaska, A.B.; Santi, P.M.; Higgins, J.D.; Cannon, S.H.
2008-01-01
Debris flow velocities are commonly back-calculated from superelevation events which require subjective estimates of radii of curvature of bends in the debris flow channel or predicted using flow equations that require the selection of appropriate rheological models and material property inputs. This research investigated difficulties associated with the use of these conventional velocity estimation methods. Radii of curvature estimates were found to vary with the extent of the channel investigated and with the scale of the media used, and back-calculated velocities varied among different investigated locations along a channel. Distinct populations of Bingham properties were found to exist between those measured by laboratory tests and those back-calculated from field data; thus, laboratory-obtained values would not be representative of field-scale debris flow behavior. To avoid these difficulties with conventional methods, a new preliminary velocity estimation method is presented that statistically relates flow velocity to the channel slope and the flow depth. This method presents ranges of reasonable velocity predictions based on 30 previously measured velocities. ?? 2008 Springer-Verlag.
Topography Modeling in Atmospheric Flows Using the Immersed Boundary Method
NASA Technical Reports Server (NTRS)
Ackerman, A. S.; Senocak, I.; Mansour, N. N.; Stevens, D. E.
2004-01-01
Numerical simulation of flow over complex geometry needs accurate and efficient computational methods. Different techniques are available to handle complex geometry. The unstructured grid and multi-block body-fitted grid techniques have been widely adopted for complex geometry in engineering applications. In atmospheric applications, terrain fitted single grid techniques have found common use. Although these are very effective techniques, their implementation, coupling with the flow algorithm, and efficient parallelization of the complete method are more involved than a Cartesian grid method. The grid generation can be tedious and one needs to pay special attention in numerics to handle skewed cells for conservation purposes. Researchers have long sought for alternative methods to ease the effort involved in simulating flow over complex geometry.
The solving of one dimensional unsteady flow with difference methods
NASA Astrophysics Data System (ADS)
Shao, Guangri; Shang, Yu; Wang, Rongsheng
In this paper, one-dimensional unsteady gas flow equations are formulated with Riemann variables as the functions of time and space to be solved. By using the 'de Haller' test and a simple pipe flow with a temperature discontinuity, the equations are solved by difference methods of first-order accuracy and second-order accuracy for non-homentropic cases. As compared with the commonly used characteristic method, the results show the above-mentioned formulation and difference methods have the advantages of consuming less computer time and being easier to reach higher order accuracy. The application to turbocharged internal combustion engines is also discussed in this paper.
Fiber optic liquid mass flow sensor and method
NASA Technical Reports Server (NTRS)
Korman, Valentin (Inventor); Gregory, Don Allen (Inventor); Wiley, John T. (Inventor); Pedersen, Kevin W. (Inventor)
2010-01-01
A method and apparatus are provided for sensing the mass flow rate of a fluid flowing through a pipe. A light beam containing plural individual wavelengths is projected from one side of the pipe across the width of the pipe so as to pass through the fluid under test. Fiber optic couplers located at least two positions on the opposite side of the pipe are used to detect the light beam. A determination is then made of the relative strengths of the light beam for each wavelength at the at least two positions and based at least in part on these relative strengths, the mass flow rate of the fluid is determined.
Compressible flow calculations employing the Galerkin/least-squares method
NASA Technical Reports Server (NTRS)
Shakib, F.; Hughes, T. J. R.; Johan, Zdenek
1989-01-01
A multielement group, domain decomposition algorithm is presented for solving linear nonsymmetric systems arising in the finite-element analysis of compressible flows employing the Galerkin/least-squares method. The iterative strategy employed is based on the generalized minimum residual (GMRES) procedure originally proposed by Saad and Shultz. Two levels of preconditioning are investigated. Applications to problems of high-speed compressible flow illustrate the effectiveness of the scheme.
AN IMMERSED BOUNDARY METHOD FOR COMPLEX INCOMPRESSIBLE FLOWS
An immersed boundary method for time-dependant, three- dimensional, incompressible flows is presented in this paper. The incompressible Navier-Stokes equations are discretized using a low-diffusion flux splitting method for the inviscid fluxes and a second order central differenc...
The flow curvature method applied to canard explosion
NASA Astrophysics Data System (ADS)
Ginoux, Jean-Marc; Llibre, Jaume
2011-11-01
The aim of this work is to establish that the bifurcation parameter value leading to a canard explosion in dimension 2 obtained by the so-called geometric singular perturbation method can be found according to the flow curvature method. This result will be then exemplified with the classical Van der Pol oscillator.
Determination of renal blood flow by thermodilution method.
Leivestad, T; Brodwall, E K; Simonsen, S
1978-09-01
The single bolus thermodilution method for measurement of renal vein blood flow was tested. In model experiments the thermodilution method was compared with graduated cylinder measurements over a flow range from 50 to 1050 ml/min. There was a good correlation between the two methods (r = 0.98) with a mean of differences of 5.2%. In eighteen patients measurements were performed in duplicate in thirty-one renal veins. Comparison was made between the first (x) and second (u) measurement--performed within 3 min. The correlation between the two was very good (r = 0.99; y = 1.03x - 11.48). In twelve patients bilateral renal vein blood flow measurements were performed simultaneous to blood flow measurement by PAH clearance. The correlation between total flow measured by thermodilution (y) and by the clearance method (x) was good (r = 0.98; y = 0.79x + 221). It is concluded that the thermodilution method requires catheterization of the renal veins, but is otherwise simple to perform, is inexpensive and gives reliable results. It is particularly advantageous when repeated measurements in the study of acute changes in renal haemodynamics is desirable. PMID:705231
Comparison of Methods for Estimating Low Flow Characteristics of Streams
Tasker, Gary D.
1987-01-01
Four methods for estimating the 7-day, 10-year and 7-day, 20-year low flows for streams are compared by the bootstrap method. The bootstrap method is a Monte Carlo technique in which random samples are drawn from an unspecified sampling distribution defined from observed data. The nonparametric nature of the bootstrap makes it suitable for comparing methods based on a flow series for which the true distribution is unknown. Results show that the two methods based on hypothetical distribution (Log-Pearson III and Weibull) had lower mean square errors than did the G. E. P. Box-D. R. Cox transformation method or the Log-W. C. Boughton method which is based on a fit of plotting positions.
Hoskinson, Reed L.; Svoboda, John M.; Bauer, William F.; Elias, Gracy
2008-05-06
A method and apparatus is provided for monitoring a flow path having plurality of different solid components flowing therethrough. For example, in the harvesting of a plant material, many factors surrounding the threshing, separating or cleaning of the plant material and may lead to the inadvertent inclusion of the component being selectively harvested with residual plant materials being discharged or otherwise processed. In accordance with the present invention the detection of the selectively harvested component within residual materials may include the monitoring of a flow path of such residual materials by, for example, directing an excitation signal toward of flow path of material and then detecting a signal initiated by the presence of the selectively harvested component responsive to the excitation signal. The detected signal may be used to determine the presence or absence of a selected plant component within the flow path of residual materials.
A nonintrusive method of quantifying flow visualization data in vortex flow fields
NASA Astrophysics Data System (ADS)
Sei, Vincent J.
1994-12-01
The High Angle of Attack Research Vehicle (HARV) as well as other similar flight test aircraft have been using smoke flow visualization techniques to characterize the vortex flow created by leading edge extensions and the forebody. With the advent of video measurement techniques, this type of flow visualization can not only provide a qualitative assessment of the flow but also a quantitative measure to be used to validate computational fluid dynamic codes and wind tunnel test. One of the major drawbacks to employing video imaging was the introduction of false motion due to camera movement in flight. A relative motion approach using fixed targets along with the flow visualization scheme was utilized to remove unwanted motion. The relative motion algorithm was tested using a laboratory test setup where cameras underwent both translational and rotational motion to simulate both wing bending and torsion. The method was effective in removing both motions with only a slight loss of accuracy.
Proposed method for measurement of flow rate in turbulent periodic pipe flow
NASA Astrophysics Data System (ADS)
Werzner, E.; Ray, S.; Trimis, D.
2011-12-01
The present investigation deals with a previously proposed flow metering technique for laminar, fully-developed, time-periodic pipe flow. Employing knowledge of the pulsation frequency-dependent relationship between the mass flow rate and the pressure gradient, the method allows reconstruction of the instantaneous mass flow rate on the basis of a recorded pressure gradient time series. In order to explore if the procedure can be extended for turbulent flows, numerical simulations for turbulent, fully-developed, sinusoidally pulsating pipe flow with low pulse amplitude have been carried out using a ν2-f turbulence model. The study covers pulsation frequencies, ranging from the quasi-steady up to the inertia-dominated frequency regime, and three cycle-averaged Reynolds numbers of 4360, 9750 and 15400. After providing the theoretical background of the flow rate reconstruction principle, the numerical model and an experimental facility for the verification of simulations are explained. The obtained results, presented in time and frequency domain, show good agreement with each other and indicate a frequency dependence, similar to that used for the signal reconstruction for laminar flows. A modified dimensionless frequency definition has been introduced, which allows a generalised representation of the results considering the influence of Reynolds number.
Gpu Implementation of Preconditioning Method for Low-Speed Flows
NASA Astrophysics Data System (ADS)
Zhang, Jiale; Chen, Hongquan
2016-06-01
An improved preconditioning method for low-Mach-number flows is implemented on a GPU platform. The improved preconditioning method employs the fluctuation of the fluid variables to weaken the influence of accuracy caused by the truncation error. The GPU parallel computing platform is implemented to accelerate the calculations. Both details concerning the improved preconditioning method and the GPU implementation technology are described in this paper. Then a set of typical low-speed flow cases are simulated for both validation and performance analysis of the resulting GPU solver. Numerical results show that dozens of times speedup relative to a serial CPU implementation can be achieved using a single GPU desktop platform, which demonstrates that the GPU desktop can serve as a cost-effective parallel computing platform to accelerate CFD simulations for low-Speed flows substantially.
A multi-domain method for subsonic viscous flows
NASA Technical Reports Server (NTRS)
Chan, Daniel C.; Sindir, Munir M.
1992-01-01
We have developed a Schwarz type domain decomposition method for a pressure base, two- and three-dimensional Navier-Stokes solver. This technique allows one to partition a flow path, which can be characterized by complex geometry and/or complicated flow physics, into smaller sub-domains according to the local geometric simplicity or estimated flow scales. We can, then, sweep the sub-domains in some order and solve the Navier-Stokes equations using as boundary conditions, along the domain interfaces, the Dirichlet conditions which are taken from the most recent update of the solution in the adjacent neighboring domains. With this technique, one can minimize the adverse effects caused by grid skewness and the stiffness problem caused by disparate flow scales. Here, we report the results of a few fundamental flow cases to demonstrate that a judicious use of the multi-domain method can offer a significant convergence acceleration over the traditional one-domain method. This method can be extended to exploit the architecture of a parallel computer to further improve the speed.
Mixed exhaust flow supersonic jet engine and method
Klees, G.W.
1993-06-08
A method of operating a supersonic jet engine installation is described comprising (a) providing an engine having a variable area air inlet means and an outlet to discharge engine exhaust; (b) providing a secondary air passageway means; (c) receiving ambient air in the air inlet means and providing the ambient air as primary air to the engine inlet and secondary air to the secondary air passageway means; (d) providing a mixing section having an inlet portion and an exit portion, utilizing the mixing section in directing the exhaust from the engine to primary convergent/divergent exit passageway segments, where the exhaust is discharged at supersonic velocity as primary flow components, and directing secondary air flow from the secondary air passageway means to secondary exit passageway segments which are interspersed with the primary segments and from which the secondary air is discharged at subsonic velocity as secondary flow components; and (e) providing an exhaust section to receive the primary and secondary flow components in a mixing region and causing the primary and secondary flow components to mix to create a supersonic mixed flow, the exhaust section having a variable area final nozzle through which the mixed flow is discharged.
Electron-Beam Diagnostic Methods for Hypersonic Flow Diagnostics
NASA Technical Reports Server (NTRS)
1994-01-01
The purpose of this work was the evaluation of the use of electron-bean fluorescence for flow measurements during hypersonic flight. Both analytical and numerical models were developed in this investigation to evaluate quantitatively flow field imaging concepts based upon the electron beam fluorescence technique for use in flight research and wind tunnel applications. Specific models were developed for: (1) fluorescence excitation/emission for nitrogen, (2) rotational fluorescence spectrum for nitrogen, (3) single and multiple scattering of electrons in a variable density medium, (4) spatial and spectral distribution of fluorescence, (5) measurement of rotational temperature and density, (6) optical filter design for fluorescence imaging, and (7) temperature accuracy and signal acquisition time requirements. Application of these models to a typical hypersonic wind tunnel flow is presented. In particular, the capability of simulating the fluorescence resulting from electron impact ionization in a variable density nitrogen or air flow provides the capability to evaluate the design of imaging instruments for flow field mapping. The result of this analysis is a recommendation that quantitative measurements of hypersonic flow fields using electron-bean fluorescence is a tractable method with electron beam energies of 100 keV. With lower electron energies, electron scattering increases with significant beam divergence which makes quantitative imaging difficult. The potential application of the analytical and numerical models developed in this work is in the design of a flow field imaging instrument for use in hypersonic wind tunnels or onboard a flight research vehicle.
Improved numerical methods for turbulent viscous recirculating flows
NASA Technical Reports Server (NTRS)
Turan, A.; Vandoormaal, J. P.
1988-01-01
The performance of discrete methods for the prediction of fluid flows can be enhanced by improving the convergence rate of solvers and by increasing the accuracy of the discrete representation of the equations of motion. This report evaluates the gains in solver performance that are available when various acceleration methods are applied. Various discretizations are also examined and two are recommended because of their accuracy and robustness. Insertion of the improved discretization and solver accelerator into a TEACH mode, that has been widely applied to combustor flows, illustrates the substantial gains to be achieved.
Continuous-flow free acid monitoring method and system
Strain, J.E.; Ross, H.H.
1980-01-11
A free acid monitoring method and apparatus is provided for continuously measuring the excess acid present in a process stream. The disclosed monitoring system and method is based on the relationship of the partial pressure ratio of water and acid in equilibrium with an acid solution at constant temperature. A portion of the process stream is pumped into and flows through the monitor under the influence of gravity and back to the process stream. A continuous flowing sample is vaporized at a constant temperature and the vapor is subsequently condensed. Conductivity measurements of the condensate produces a nonlinear response function from which the free acid molarity of the sample process stream is determined.
Continuous-flow free acid monitoring method and system
Strain, James E.; Ross, Harley H.
1981-01-01
A free acid monitoring method and apparatus is provided for continuously measuring the excess acid present in a process stream. The disclosed monitoring system and method is based on the relationship of the partial pressure ratio of water and acid in equilibrium with an acid solution at constant temperature. A portion of the process stream is pumped into and flows through the monitor under the influence of gravity and back to the process stream. A continuous flowing sample is vaporized at a constant temperature and the vapor is subsequently condensed. Conductivity measurements of the condensate produces a nonlinear response function from which the free acid molarity of the sample process stream is determined.
Systems and methods for rebalancing redox flow battery electrolytes
Pham, Ai Quoc; Chang, On Kok
2015-03-17
Various methods of rebalancing electrolytes in a redox flow battery system include various systems using a catalyzed hydrogen rebalance cell configured to minimize the risk of dissolved catalyst negatively affecting flow battery performance. Some systems described herein reduce the chance of catalyst contamination of RFB electrolytes by employing a mediator solution to eliminate direct contact between the catalyzed membrane and the RFB electrolyte. Other methods use a rebalance cell chemistry that maintains the catalyzed electrode at a potential low enough to prevent the catalyst from dissolving.
Finite element method application for turbulent and transitional flow
NASA Astrophysics Data System (ADS)
Sváček, Petr
2016-03-01
This paper is interested in numerical simulations of the interaction of the fluid flow with an airfoil. Particularly, the problem of the turbulent flow around the airfoil with elastic support is considered. The main attention is paid to the numerical approximation of the flow problem using the finite element approximations. The laminar - turbulence transition of the flow on the surface airfoil is considered. The chois of the transition model is discussed. The transition model based on the two equation k-ω turbulence model is used. The structure motion is described with the aid of two degrees of freedom. The motion of the computational domain is treated with the aid of the arbitrary Lagrangian-Eulerian method. Numerical results are shown.
Combustor air flow control method for fuel cell apparatus
Clingerman, Bruce J.; Mowery, Kenneth D.; Ripley, Eugene V.
2001-01-01
A method for controlling the heat output of a combustor in a fuel cell apparatus to a fuel processor where the combustor has dual air inlet streams including atmospheric air and fuel cell cathode effluent containing oxygen depleted air. In all operating modes, an enthalpy balance is provided by regulating the quantity of the air flow stream to the combustor to support fuel cell processor heat requirements. A control provides a quick fast forward change in an air valve orifice cross section in response to a calculated predetermined air flow, the molar constituents of the air stream to the combustor, the pressure drop across the air valve, and a look up table of the orifice cross sectional area and valve steps. A feedback loop fine tunes any error between the measured air flow to the combustor and the predetermined air flow.
Method for selectively controlling flow across slotted liners
Peavy, M.A.; Dees, J.M.
1993-08-31
A process is described for decreasing flow rate across the radial boundary of a selected interval in a well bore containing a slotted liner comprising: placing an explosive and an internally catalyzed resin solution inside an elongated container; locating the elongated container opposite the selected interval in the well bore where flow rate through the slotted liner is to be decreased; firing the explosive; and allowing the resin to cure on the slotted liner before initiating flow through the well. A method is described for decreasing production of unwanted fluids from a horizontal well containing a slotted liner comprising: placing an explosive and an internally catalyzed resin inside an elongated container; placing the elongated container opposite an interval in the horizontal well where unwanted fluid is entering the well bore through the slotted liner; firing the explosive; and permitting the resin to cure on the slotted liner before initiating flow in the well.
A simulated dye method for flow visualization with a computational model for blood flow.
Kim, T; Cheer, A Y; Dwyer, H A
2004-08-01
A numerical dye method for the visualization of unsteady three-dimensional flow calculations is introduced by coupling the unsteady convection-diffusion equation to the Navier-Stokes equation for mass and momentum. This system of equations is descretized using a finite volume projection-like algorithm with generalized coordinates and overset grids. A powerful pressure prediction method is used to accelerate the convergence of the Pressure Poisson equation. To demonstrate the visualization technique, blood flow through the aortic arch region and the three main arterial branches is computed using various Womersley numbers. In this technique, parcels of fluid are followed in time as a function of the cardiac cycle without having to track individual particles, which in turn aids us to better understand some important aspects of the three-dimensionality of the developing unsteady flow. Using this numerical dye method we analyze the strength of the cross flow during the cardiac cycle, the relationship between the penetration of blood into the aortic branches from its relative position in the ascending aortic region and the effects of the Womersley parameter. This technique can be very useful in the design and development of stents where the topology of the device would require understanding where the blood emanating from the heart ends up at the end of the cardiac cycle. Moreover, this method could be useful in investigating the influence of flow and geometry on the local introduction of medication.
Methods for estimating low-flow statistics for Massachusetts streams
Ries, Kernell G.; Friesz, Paul J.
2000-01-01
Methods and computer software are described in this report for determining flow duration, low-flow frequency statistics, and August median flows. These low-flow statistics can be estimated for unregulated streams in Massachusetts using different methods depending on whether the location of interest is at a streamgaging station, a low-flow partial-record station, or an ungaged site where no data are available. Low-flow statistics for streamgaging stations can be estimated using standard U.S. Geological Survey methods described in the report. The MOVE.1 mathematical method and a graphical correlation method can be used to estimate low-flow statistics for low-flow partial-record stations. The MOVE.1 method is recommended when the relation between measured flows at a partial-record station and daily mean flows at a nearby, hydrologically similar streamgaging station is linear, and the graphical method is recommended when the relation is curved. Equations are presented for computing the variance and equivalent years of record for estimates of low-flow statistics for low-flow partial-record stations when either a single or multiple index stations are used to determine the estimates. The drainage-area ratio method or regression equations can be used to estimate low-flow statistics for ungaged sites where no data are available. The drainage-area ratio method is generally as accurate as or more accurate than regression estimates when the drainage-area ratio for an ungaged site is between 0.3 and 1.5 times the drainage area of the index data-collection site. Regression equations were developed to estimate the natural, long-term 99-, 98-, 95-, 90-, 85-, 80-, 75-, 70-, 60-, and 50-percent duration flows; the 7-day, 2-year and the 7-day, 10-year low flows; and the August median flow for ungaged sites in Massachusetts. Streamflow statistics and basin characteristics for 87 to 133 streamgaging stations and low-flow partial-record stations were used to develop the equations. The
NASA Astrophysics Data System (ADS)
Jang, Jaeseong; Ahn, Chi Young; Jeon, Kiwan; Choi, Jung-il; Lee, Changhoon; Seo, Jin Keun
2015-03-01
A reconstruction method is proposed here to quantify the distribution of blood flow velocity fields inside the left ventricle from color Doppler echocardiography measurement. From 3D incompressible Navier- Stokes equation, a 2D incompressible Navier-Stokes equation with a mass source term is derived to utilize the measurable color flow ultrasound data in a plane along with the moving boundary condition. The proposed model reflects out-of-plane blood flows on the imaging plane through the mass source term. For demonstrating a feasibility of the proposed method, we have performed numerical simulations of the forward problem and numerical analysis of the reconstruction method. First, we construct a 3D moving LV region having a specific stroke volume. To obtain synthetic intra-ventricular flows, we performed a numerical simulation of the forward problem of Navier-Stokes equation inside the 3D moving LV, computed 3D intra-ventricular velocity fields as a solution of the forward problem, projected the 3D velocity fields on the imaging plane and took the inner product of the 2D velocity fields on the imaging plane and scanline directional velocity fields for synthetic scanline directional projected velocity at each position. The proposed method utilized the 2D synthetic projected velocity data for reconstructing LV blood flow. By computing the difference between synthetic flow and reconstructed flow fields, we obtained the averaged point-wise errors of 0.06 m/s and 0.02 m/s for u- and v-components, respectively.
McGrail, Bernard P.; Martin, Paul F.; Lindenmeier, Clark W.
1999-01-01
The present invention is a method and apparatus for measuring coupled flow, transport and reaction processes under liquid unsaturated flow conditions. The method and apparatus of the present invention permit distinguishing individual precipitation events and their effect on dissolution behavior isolated to the specific event. The present invention is especially useful for dynamically measuring hydraulic parameters when a chemical reaction occurs between a particulate material and either liquid or gas (e.g. air) or both, causing precipitation that changes the pore structure of the test material.
Validation of an Impedance Education Method in Flow
NASA Technical Reports Server (NTRS)
Watson, Willie R.; Jones, Michael G.; Parrott, Tony L.
2004-01-01
This paper reports results of a research effort to validate a method for educing the normal incidence impedance of a locally reacting liner, located in a grazing incidence, nonprogressive acoustic wave environment with flow. The results presented in this paper test the ability of the method to reproduce the measured normal incidence impedance of a solid steel plate and two soft test liners in a uniform flow. The test liners are known to be locally react- ing and exhibit no measurable amplitude-dependent impedance nonlinearities or flow effects. Baseline impedance spectra for these liners were therefore established from measurements in a conventional normal incidence impedance tube. A key feature of the method is the expansion of the unknown impedance function as a piecewise continuous polynomial with undetermined coefficients. Stewart's adaptation of the Davidon-Fletcher-Powell optimization algorithm is used to educe the normal incidence impedance at each Mach number by optimizing an objective function. The method is shown to reproduce the measured normal incidence impedance spectrum for each of the test liners, thus validating its usefulness for determining the normal incidence impedance of test liners for a broad range of source frequencies and flow Mach numbers. Nomenclature
Method and apparatus for characterizing blood flow through the heart
Dam, N.G.; Gray, R.I.; Kramer, H.H.; Picunko, T.
1981-10-13
An automated method and improved device provide a measurement of blood flow through the heart by the detection and analysis of radioactivity emitted by a radioactive tracer introduced into a patient's bloodstream. Real time, cardiac cycle by cardiac cycle information is processed and displayed to provide diagnostically useful information on an essentially ongoing basis as the patient is being tested.
Simple method of supersonic flow visualization using smoke
NASA Astrophysics Data System (ADS)
Miller, L. S.; Irani, E.
1992-01-01
A modified smoke wire visualization method for application in high-speed and supersonic flows has been developed which produces a large number of fine smoke filaments that do not dissipate. This new technique is easily implemented in an open-inlet induction-type supersonic wind tunnel. Shock waves and expansion regions are clearly identified by the smoke filament behavior.
The numerical methods for the fluid flow of UCMCWS
Zhang Wenfu; Li Hui; Zhu Shuquan; Wang Zuna
1997-12-31
As an alternative for diesel oil for internal combustion engines, the fluid flow state of Ultra Clean Micronized Coal-Water Slurry (UCMCWS) in mini pipe and nozzle of a diesel engine must be known. In the laboratory three kinds of UCMCWS have been made with coal containing less than 0.8% ash, viscosity less than 600 mPa.s and concentration between 50% and 56%. Because the UCMCWS is a non-Newtonian fluid, there are no analytical resolution for pipe flow, especially in inlet and outlet sections. In this case using the numerical methods to research the flow state of UCMCWS is a useful method. Using the method of finite element, the flow state of UCMCWS in inlet and outlet sections (similar to a nozzle) have been studied. The distribution of velocity at different pressures of UCMCWS in outlet and inlet sections have been obtained. The result of the numerical methods is the efficient base for the pipe and nozzle design.
Multicomponent-flow analyses by multimode method of characteristics
Lai, Chintu
1994-01-01
For unsteady open-channel flows having N interacting unknown variables, a system of N mutually independent, partial differential equations can be used to describe the flow-field. The system generally belongs to marching-type problems and permits transformation into characteristic equations that are associated with N distinct characteristics directions. Because characteristics can be considered 'wave' or 'disturbance' propagation, a fluvial system so described can be viewed as adequately definable using these N component waves. A numerical algorithm to solve the N families of characteristics can then be introduced for formulation of an N-component flow-simulation model. The multimode method of characteristics (MMOC), a new numerical scheme that has a combined capacity of several specified-time-interval (STI) schemes of the method of characteristics, makes numerical modeling of such N-component riverine flows feasible and attainable. Merging different STI schemes yields different kinds of MMOC schemes, for which two kinds are displayed herein. With the MMOC, each characteristics is dynamically treated by an appropriate numerical mode, which should lead to an effective and suitable global simulation, covering various types of unsteady flow. The scheme is always linearly stable and its numerical accuracy can be systematically analyzed. By increasing the N value, one can develop a progressively sophisticated model that addresses increasingly complex river-mechanics problems.
Method for measuring Doppler shifts in arc-heated flows.
Aeschliman, D P; Hill, R A
1972-01-01
A novel method of determining both the Doppler and Stark shifts in a single measurement of spectral lines emitted by the arc-heated flow from a plasma jet has been successfully demonstrated. The method uses a spherical mirror arranged with its optical axis coincident with the optical axis of a Fabry-Perot interferometer and with its center of curvature at the center line of the flow. The common optical axis lies at an angle to the flow. With this system, both red-and blue-shifted line profiles are recorded in the same spectral scan. If conditions are such that the red-and blue-shifted profiles are not resolvable, the blue-shifted component is chopped so that the recorded signal consists of the envelopes of both the red-shifted profile and the superimposed red-and blue-shifted profiles. The wavelength difference between the blue-and red-shifted line profiles is exactly twice the Doppler shift integrated along a line of sight through the flow and is independent of a Stark shift. The Stark shift is given by the wavelength difference between the absolute line center and the midpoint of the red-and blue-shifted lines. Abel inversion of integrated line shift data has yielded radial velocity profiles to an accuracy of +/-3% in a supersonic, arc-heated argon flow.
Methods of Visually Determining the Air Flow Around Airplanes
NASA Technical Reports Server (NTRS)
Gough, Melvin N; Johnson, Ernest
1932-01-01
This report describes methods used by the National Advisory Committee for Aeronautics to study visually the air flow around airplanes. The use of streamers, oil and exhaust gas streaks, lampblack and kerosene, powdered materials, and kerosene smoke is briefly described. The generation and distribution of smoke from candles and from titanium tetrachloride are described in greater detail because they appear most advantageous for general application. Examples are included showing results of the various methods.
A Multi-domain Spectral Method for Supersonic Reactive Flows
NASA Technical Reports Server (NTRS)
Don, Wai-Sun; Gottlieb, David; Jung, Jae-Hun; Bushnell, Dennis M. (Technical Monitor)
2002-01-01
This paper has a dual purpose: it presents a multidomain Chebyshev method for the solution of the two-dimensional reactive compressible Navier-Stokes equations, and it reports the results of the application of this code to the numerical simulations of high Mach number reactive flows in recessed cavity. The computational method utilizes newly derived interface boundary conditions as well as an adaptive filtering technique to stabilize the computations. The results of the simulations are relevant to recessed cavity flameholders.
Simple numerical method for predicting steady compressible flows
NASA Technical Reports Server (NTRS)
Vonlavante, Ernst; Nelson, N. Duane
1986-01-01
A numerical method for solving the isenthalpic form of the governing equations for compressible viscous and inviscid flows was developed. The method was based on the concept of flux vector splitting in its implicit form. The method was tested on several demanding inviscid and viscous configurations. Two different forms of the implicit operator were investigated. The time marching to steady state was accelerated by the implementation of the multigrid procedure. Its various forms very effectively increased the rate of convergence of the present scheme. High quality steady state results were obtained in most of the test cases; these required only short computational times due to the relative efficiency of the basic method.
Traffic Flow Management Using Aggregate Flow Models and the Development of Disaggregation Methods
NASA Technical Reports Server (NTRS)
Sun, Dengfeng; Sridhar, Banavar; Grabbe, Shon
2010-01-01
A linear time-varying aggregate traffic flow model can be used to develop Traffic Flow Management (tfm) strategies based on optimization algorithms. However, there are no methods available in the literature to translate these aggregate solutions into actions involving individual aircraft. This paper describes and implements a computationally efficient disaggregation algorithm, which converts an aggregate (flow-based) solution to a flight-specific control action. Numerical results generated by the optimization method and the disaggregation algorithm are presented and illustrated by applying them to generate TFM schedules for a typical day in the U.S. National Airspace System. The results show that the disaggregation algorithm generates control actions for individual flights while keeping the air traffic behavior very close to the optimal solution.
A Three-Dimensional Vortex Sheet Method for Multiphase Flows
NASA Astrophysics Data System (ADS)
Stock, Mark; Dahm, Werner; Tryggvason, Gretar
2002-11-01
Previous work on a three-dimensional vortex-in-cell method is extended to include baroclinic vorticity generation in flows with large density ratios. A vortex sheet discretization is used both to maintain the boundary between different fluids or fluid phases, and to provide for a divergence-free vorticity field at all times. Automatic insertion and deletion of triangular elements allow the vortex sheet to maintain its connectivity and resolution during the simulation, despite extensive stretching of the material surface. The VIC grid provides regularization, and the simulation is inviscid at resolved scales. Computational results for flows with weak and strong density variations are presented.
A multilevel adaptive projection method for unsteady incompressible flow
NASA Technical Reports Server (NTRS)
Howell, Louis H.
1993-01-01
There are two main requirements for practical simulation of unsteady flow at high Reynolds number: the algorithm must accurately propagate discontinuous flow fields without excessive artificial viscosity, and it must have some adaptive capability to concentrate computational effort where it is most needed. We satisfy the first of these requirements with a second-order Godunov method similar to those used for high-speed flows with shocks, and the second with a grid-based refinement scheme which avoids some of the drawbacks associated with unstructured meshes. These two features of our algorithm place certain constraints on the projection method used to enforce incompressibility. Velocities are cell-based, leading to a Laplacian stencil for the projection which decouples adjacent grid points. We discuss features of the multigrid and multilevel iteration schemes required for solution of the resulting decoupled problem. Variable-density flows require use of a modified projection operator--we have found a multigrid method for this modified projection that successfully handles density jumps of thousands to one. Numerical results are shown for the 2D adaptive and 3D variable-density algorithms.
Analysis of Motorcycle Weave Mode by using Energy Flow Method
NASA Astrophysics Data System (ADS)
Marumo, Yoshitaka; Katayama, Tsuyoshi
The activation mechanism of motorcycle weave mode is clarified within the framework of the energy flow method, which calculates energy flow of mechanical forces in each motion. It is demonstrated that only a few mechanical forces affect the stability of the weave mode from among a total of about 40 mechanical forces. The activation of the lateral, yawing and rolling motions destabilize the weave mode, while activation of the steering motion stabilizes the weave mode. A detailed investigation of the energy flow of the steering motion reveals that the steering motion plays an important role in clarifying the characteristics of the weave mode. As activation of the steering motion progresses the phase of the front tire side force, and the weave mode is consequently stabilized. This paper provides a design guide for stabilizing the weave mode and the wobble mode compatibility.
Method for Studying Microbial Biofilms in Flowing-Water Systems
Pedersen, Karsten
1982-01-01
A method for the study of microbial biofilms in flowing-water systems was developed with special reference to the flow conditions in electrochemical concentration cells. Seawater was circulated in a semiclosed flow system through biofilm reactors (3 cm s−1) with microscope cover slips arranged in lamellar piles parallel with the flow. At fixed time intervals cover slips with their biofilm were removed from the pile, stained with crystal violet, and mounted on microscope slides. The absorbances of the slides were measured at 590 nm and plotted against time to give microbial biofilm development. From calibration experiments a staining time of 1 min and a rinse time of 10 min in a tap water flow (3 cm s−1) were considered sufficient. When an analysis of variance was performed on biofilm development data, 78% of the total variance was found to be due to random natural effects; the rest could be explained by experimental effects. The absorbance values correlated well with protein N, dry weight, and organic weight in two biofilm experiments, one with a biofilm with a high (75%) and one with a low (∼25%, normal) inorganic content. Comparisons of regression lines revealed that the absorbance of the stained biofilms was an estimate closely related to biofilm dry weight. PMID:16345929
Numerical Methods and Simulations of Complex Multiphase Flows
NASA Astrophysics Data System (ADS)
Brady, Peter
Multiphase flows are an important part of many natural and technological phenomena such as ocean-air coupling (which is important for climate modeling) and the atomization of liquid fuel jets in combustion engines. The unique challenges of multiphase flow often make analytical solutions to the governing equations impossible and experimental investigations very difficult. Thus, high-fidelity numerical simulations can play a pivotal role in understanding these systems. This dissertation describes numerical methods developed for complex multiphase flows and the simulations performed using these methods. First, the issue of multiphase code verification is addressed. Code verification answers the question "Is this code solving the equations correctly?" The method of manufactured solutions (MMS) is a procedure for generating exact benchmark solutions which can test the most general capabilities of a code. The chief obstacle to applying MMS to multiphase flow lies in the discontinuous nature of the material properties at the interface. An extension of the MMS procedure to multiphase flow is presented, using an adaptive marching tetrahedron style algorithm to compute the source terms near the interface. Guidelines for the use of the MMS to help locate coding mistakes are also detailed. Three multiphase systems are then investigated: (1) the thermocapillary motion of three-dimensional and axisymmetric drops in a confined apparatus, (2) the flow of two immiscible fluids completely filling an enclosed cylinder and driven by the rotation of the bottom endwall, and (3) the atomization of a single drop subjected to a high shear turbulent flow. The systems are simulated numerically by solving the full multiphase Navier-Stokes equations coupled to the various equations of state and a level set interface tracking scheme based on the refined level set grid method. The codes have been parallelized using MPI in order to take advantage of today's very large parallel computational
Circulation methods in unsteady and three-dimensional flows
NASA Astrophysics Data System (ADS)
Yuan, Jiankun
The largely unstudied extension of ultrasonic circulation measurement techniques (UCMT) to determine instantaneous lift in unsteady and three-dimensional flows has been addressed in this work. A combined analytical-numerical-experimental approach was undertaken with the goal of developing methods to properly convert the measurable time-dependent bound circulation to instantaneous lift force in unsteady flows. The measurement of mean sectional lift distribution along structure spans in three-dimensional flows was also studied. An unsteady correction method for thin airfoils was developed analytically and validated numerically (with finite element solutions) to properly convert bound circulation to instantaneous lift based on unsteady potential flow theory. Results show that the unsteady correction method can provide increased accuracy for unsteady lift prediction over the Kutta-Joukowski method used in previous unsteady flow studies. The unsteady correction model generally should be included for instantaneous lift prediction as long as the bound circulation is time-dependent. Using the same framework, we also studied determination of instantaneous lift forces on stationary bluff bodies (circular cylinders) at low Reynolds number (Re = 100). Various force models, including an approximate vortex force model, were studied. A new unsteady model, similar to that developed for the thin airfoils, using instantaneous bound circulation values, was proposed. Another important issue studied in this thesis is the effect of acoustic path sensitivity on bound circulation determination, which we found to be crucial for accurately predicting the instantaneous lift in both unsteady flat plate and cylinder flows. Proper path selection should take into account the location of boundary layers, attached and shed vortices. These findings will be useful in future experimental design of UCMT, PIV and LDV methods. Finally, we used the UCMT method to experimentally study the mean spatial
Pseudo-compressibility methods for the incompressible flow equations
NASA Technical Reports Server (NTRS)
Turkel, Eli; Arnone, A.
1993-01-01
Preconditioning methods to accelerate convergence to a steady state for the incompressible fluid dynamics equations are considered. The analysis relies on the inviscid equations. The preconditioning consists of a matrix multiplying the time derivatives. Thus the steady state of the preconditioned system is the same as the steady state of the original system. The method is compared to other types of pseudo-compressibility. For finite difference methods preconditioning can change and improve the steady state solutions. An application to viscous flow around a cascade with a non-periodic mesh is presented.
A fictitious domain method for particulate flows with heat transfer
NASA Astrophysics Data System (ADS)
Yu, Zhaosheng; Shao, Xueming; Wachs, Anthony
2006-09-01
The distributed-Lagrange-multiplier/fictitious-domain (DLM/FD) method of Glowinski et al. [R. Glowinski, T.-W. Pan, T.I. Hesla, D.D. Joseph, A distributed Lagrange multiplier/fictitious domain method for particulate flows, Int. J. Multiphase Flow 25 (1999) 755-794] is extended to deal with heat transfer in particulate flows in two dimensions. The Boussinesq approximation is employed for the coupling between the flow and temperature fields. The fluid-flow equations are solved with the finite-difference projection method on a half-staggered grid. In our operator splitting scheme, the Lagrange multipliers at the previous time level are kept in the fluid equations, and the new Lagrange multipliers for the rigid-body motion constraint and the Dirichlet temperature boundary condition are determined from the reduced saddle-point problem, whereas a very simple scheme based on the fully explicit computation of the Lagrange multiplier is proposed for the problem in which the solid heat conduction inside the particle boundary is also considered. Our code for the case of fixed temperature on the immersed boundary is verified by comparing favorably our results on the natural convection driven by a hot cylinder eccentrically placed in a square box and on the sedimentation of a cold circular particle in a vertical channel to the data in the literature. The code for the case of freely varying temperature on the boundaries of freely moving particles is applied to analyze the motion of a catalyst particle in a box and in particular the heat conductivities of nanofluids and sheared non-colloidal suspensions, respectively. Our preliminary computational results support the argument that the micro-heat-convection in the fluids is primarily responsible for the unusually high heat conductivity of nanofluids. It is shown that the Peclet number plays a negative role in the diffusion-related heat conductivity of a sheared non-colloidal suspension, whereas the Reynolds number does the
Vectorization of a particle simulation method for hypersonic rarefied flow
NASA Technical Reports Server (NTRS)
Mcdonald, Jeffrey D.; Baganoff, Donald
1988-01-01
An efficient particle simulation technique for hypersonic rarefied flows is presented at an algorithmic and implementation level. The implementation is for a vector computer architecture, specifically the Cray-2. The method models an ideal diatomic Maxwell molecule with three translational and two rotational degrees of freedom. Algorithms are designed specifically for compatibility with fine grain parallelism by reducing the number of data dependencies in the computation. By insisting on this compatibility, the method is capable of performing simulation on a much larger scale than previously possible. A two-dimensional simulation of supersonic flow over a wedge is carried out for the near-continuum limit where the gas is in equilibrium and the ideal solution can be used as a check on the accuracy of the gas model employed in the method. Also, a three-dimensional, Mach 8, rarefied flow about a finite-span flat plate at a 45 degree angle of attack was simulated. It utilized over 10 to the 7th particles carried through 400 discrete time steps in less than one hour of Cray-2 CPU time. This problem was chosen to exhibit the capability of the method in handling a large number of particles and a true three-dimensional geometry.
The New Performance Calculation Method of Fouled Axial Flow Compressor
Xu, Hong
2014-01-01
Fouling is the most important performance degradation factor, so it is necessary to accurately predict the effect of fouling on engine performance. In the previous research, it is very difficult to accurately model the fouled axial flow compressor. This paper develops a new performance calculation method of fouled multistage axial flow compressor based on experiment result and operating data. For multistage compressor, the whole compressor is decomposed into two sections. The first section includes the first 50% stages which reflect the fouling level, and the second section includes the last 50% stages which are viewed as the clean stage because of less deposits. In this model, the performance of the first section is obtained by combining scaling law method and linear progression model with traditional stage stacking method; simultaneously ambient conditions and engine configurations are considered. On the other hand, the performance of the second section is calculated by averaged infinitesimal stage method which is based on Reynolds' law of similarity. Finally, the model is successfully applied to predict the 8-stage axial flow compressor and 16-stage LM2500-30 compressor. The change of thermodynamic parameters such as pressure ratio, efficiency with the operating time, and stage number is analyzed in detail. PMID:25197717
An upwind nodal integral method for incompressible fluid flow
Esser, P.D. ); Witt, R.J. )
1993-05-01
An upwind nodal solution method is developed for the steady, two-dimensional flow of an incompressible fluid. The formulation is based on the nodal integral method, which uses transverse integrations, analytical solutions of the one-dimensional averaged equations, and node-averaged uniqueness constraints to derive the discretized nodal equations. The derivation introduces an exponential upwind bias by retaining the streamwise convection term in the homogeneous part of the transverse-integrated convection-diffusion equation. The method is adapted to the stream function-vorticity form of the Navier-Stokes equations, which are solved over a nonstaggered nodal mesh. A special nodal scheme is used for the Poisson stream function equation to properly account for the exponentially varying vorticity source. Rigorous expressions for the velocity components and the no-slip vorticity boundary condition are derived from the stream function formulation. The method is validated with several benchmark problems. An idealized purely convective flow of a scalar step function indicates that the nodal approximation errors are primarily dispersive, not dissipative, in nature. Results for idealized and actual recirculating driven-cavity flows reveal a significant reduction in false diffusion compared with conventional finite difference techniques.
The new performance calculation method of fouled axial flow compressor.
Yang, Huadong; Xu, Hong
2014-01-01
Fouling is the most important performance degradation factor, so it is necessary to accurately predict the effect of fouling on engine performance. In the previous research, it is very difficult to accurately model the fouled axial flow compressor. This paper develops a new performance calculation method of fouled multistage axial flow compressor based on experiment result and operating data. For multistage compressor, the whole compressor is decomposed into two sections. The first section includes the first 50% stages which reflect the fouling level, and the second section includes the last 50% stages which are viewed as the clean stage because of less deposits. In this model, the performance of the first section is obtained by combining scaling law method and linear progression model with traditional stage stacking method; simultaneously ambient conditions and engine configurations are considered. On the other hand, the performance of the second section is calculated by averaged infinitesimal stage method which is based on Reynolds' law of similarity. Finally, the model is successfully applied to predict the 8-stage axial flow compressor and 16-stage LM2500-30 compressor. The change of thermodynamic parameters such as pressure ratio, efficiency with the operating time, and stage number is analyzed in detail.
An efficient method for enumerating oral spirochetes using flow cytometry.
Orth, Rebecca; O'Brien-Simpson, Neil; Dashper, Stuart; Walsh, Katrina; Reynolds, Eric
2010-02-01
Spirochetes, such as Treponema denticola, are thin walled, helical, motile bacteria. They are notoriously difficult to enumerate due to their thinness and the difficulties associated with culturing them. Here we have developed a modified oral bacterial growth medium (OBGM) that significantly improves the cultivation of T. denticola compared with a previously published growth medium. Three methods for the enumeration of T. denticola, semi-solid growth medium colony-forming unit (CFU) counts, DNA analysis and flow cytometry, are described and compared. Enumeration of T. denticola using the semi-solid agar method resulted in a positive linear relationship with absorbance of the culture (R(2)=0.9423). However, the semi-solid agar method was found to consistently underestimate (by 50 fold) the T. denticola cell density compared to previously published data. DNA analysis of T. denticola cultures reliably and consistently resulted in a positive linear relationship with absorbance (R(2)=0.9360), giving a calculated cell density of 6.9 x 10(8)cells/mL at an absorbance of 0.2 at 650 nm. Flow cytometry was also found to result in a positive linear relationship with absorbance (R(2)=0.9874), giving a calculated cell density of 6.6 x 10(8)cells/mL at an absorbance of 0.2 at 650 nm. In comparing all of these enumeration methods, the flow cytometry method was found to have distinct advantages, as it is accurate, rapid, and could distinguish between live and dead bacteria. Thus flow cytometry is a recommended means for the rapid and reliable enumeration of viable spirochetes from culture.
A level-set method for interfacial flows with surfactant
NASA Astrophysics Data System (ADS)
Xu, Jian-Jun; Li, Zhilin; Lowengrub, John; Zhao, Hongkai
2006-03-01
A level-set method for the simulation of fluid interfaces with insoluble surfactant is presented in two-dimensions. The method can be straightforwardly extended to three-dimensions and to soluble surfactants. The method couples a semi-implicit discretization for solving the surfactant transport equation recently developed by Xu and Zhao [J. Xu, H. Zhao. An Eulerian formulation for solving partial differential equations along a moving interface, J. Sci. Comput. 19 (2003) 573-594] with the immersed interface method originally developed by LeVeque and Li and [R. LeVeque, Z. Li. The immersed interface method for elliptic equations with discontinuous coefficients and singular sources, SIAM J. Numer. Anal. 31 (1994) 1019-1044] for solving the fluid flow equations and the Laplace-Young boundary conditions across the interfaces. Novel techniques are developed to accurately conserve component mass and surfactant mass during the evolution. Convergence of the method is demonstrated numerically. The method is applied to study the effects of surfactant on single drops, drop-drop interactions and interactions among multiple drops in Stokes flow under a steady applied shear. Due to Marangoni forces and to non-uniform Capillary forces, the presence of surfactant results in larger drop deformations and more complex drop-drop interactions compared to the analogous cases for clean drops. The effects of surfactant are found to be most significant in flows with multiple drops. To our knowledge, this is the first time that the level-set method has been used to simulate fluid interfaces with surfactant.
Unstructured Mesh Methods for the Simulation of Hypersonic Flows
NASA Technical Reports Server (NTRS)
Peraire, Jaime; Bibb, K. L. (Technical Monitor)
2001-01-01
This report describes the research work undertaken at the Massachusetts Institute of Technology. The aim of this research is to identify effective algorithms and methodologies for the efficient and routine solution of hypersonic viscous flows about re-entry vehicles. For over ten years we have received support from NASA to develop unstructured mesh methods for Computational Fluid Dynamics. As a result of this effort a methodology based on the use, of unstructured adapted meshes of tetrahedra and finite volume flow solvers has been developed. A number of gridding algorithms flow solvers, and adaptive strategies have been proposed. The most successful algorithms developed from the basis of the unstructured mesh system FELISA. The FELISA system has been extensively for the analysis of transonic and hypersonic flows about complete vehicle configurations. The system is highly automatic and allows for the routine aerodynamic analysis of complex configurations starting from CAD data. The code has been parallelized and utilizes efficient solution algorithms. For hypersonic flows, a version of the, code which incorporates real gas effects, has been produced. One of the latest developments before the start of this grant was to extend the system to include viscous effects. This required the development of viscous generators, capable of generating the anisotropic grids required to represent boundary layers, and viscous flow solvers. In figures I and 2, we show some sample hypersonic viscous computations using the developed viscous generators and solvers. Although these initial results were encouraging, it became apparent that in order to develop a fully functional capability for viscous flows, several advances in gridding, solution accuracy, robustness and efficiency were required. As part of this research we have developed: 1) automatic meshing techniques and the corresponding computer codes have been delivered to NASA and implemented into the GridEx system, 2) a finite
Computational flow development for unsteady viscous flows: Foundation of the numerical method
NASA Technical Reports Server (NTRS)
Bratanow, T.; Spehert, T.
1978-01-01
A procedure is presented for effective consideration of viscous effects in computational development of high Reynolds number flows. The procedure is based on the interpretation of the Navier-Stokes equations as vorticity transport equations. The physics of the flow was represented in a form suitable for numerical analysis. Lighthill's concept for flow development for computational purposes was adapted. The vorticity transport equations were cast in a form convenient for computation. A statement for these equations was written using the method of weighted residuals and applying the Galerkin criterion. An integral representation of the induced velocity was applied on the basis of the Biot-Savart law. Distribution of new vorticity, produced at wing surfaces over small computational time intervals, was assumed to be confined to a thin region around the wing surfaces.
Axial flow heat exchanger devices and methods for heat transfer using axial flow devices
Koplow, Jeffrey P.
2016-02-16
Systems and methods described herein are directed to rotary heat exchangers configured to transfer heat to a heat transfer medium flowing in substantially axial direction within the heat exchangers. Exemplary heat exchangers include a heat conducting structure which is configured to be in thermal contact with a thermal load or a thermal sink, and a heat transfer structure rotatably coupled to the heat conducting structure to form a gap region between the heat conducting structure and the heat transfer structure, the heat transfer structure being configured to rotate during operation of the device. In example devices heat may be transferred across the gap region from a heated axial flow of the heat transfer medium to a cool stationary heat conducting structure, or from a heated stationary conducting structure to a cool axial flow of the heat transfer medium.
Multiple light scattering methods for multiphase flow diagnostics
NASA Astrophysics Data System (ADS)
Estevadeordal, Jordi
2015-11-01
Multiphase flows of gases and liquids containing droplets, bubbles, or particulates present light scattering imaging challenges due to the interference from each phase, such as secondary reflections, extinctions, absorptions, and refractions. These factors often prevent the unambiguous detection of each phase and also produce undesired beam steering. The effects can be especially complex in presence of dense phases, multispecies flows, and high pressure environments. This investigation reports new methods for overcoming these effects for quantitative measurements of velocity, density, and temperature fields. The methods are based on light scattering techniques combining Mie and filtered Rayleigh scattering and light extinction analyses and measurements. The optical layout is designed to perform multiple property measurements with improved signal from each phase via laser spectral and polarization characterization, etalon decontamination, and use of multiple wavelengths and imaging detectors.
Research on stochastic power-flow study methods. Final report
Heydt, G. T.
1981-01-01
A general algorithm to determine the effects of uncertainty in bus load and generation on the output of conventional power flow analysis is presented. The use of statistical moments is presented and developed as a means for representing the stochastic process. Statistical moments are used to describe the uncertainties, and facilitate the calculations of single and multivarlate probability density functions of input and output variables. The transformation of the uncertainty through the power flow equations is made by the expansion of the node equations in a multivariate Taylor series about an expected operating point. The series is truncated after the second order terms. Since the power flow equations are nonlinear, the expected values of output quantities is in general not the solution to the conventional load flow problem using expected values of input quantities. The second order transformation offers a correction vector and allows the consideration of larger uncertainties which have caused significant error in the current linear transformation algorithms. Voltage controlled busses are included with consideration of upper and lower limits. The finite reactive power available at generation sites, and fixed ranges of transformer tap movement may have a significant effect on voltage and line power flow statistics. A method is given which considers limitation constraints in the evaluation of all output quantities. The bus voltages, line power flows, transformer taps, and generator reactive power requirements are described by their statistical moments. Their values are expressed in terms of the probability that they are above or below specified limits, and their expected values given that they do fall outside the limits. Thus the algorithm supplies information about severity of overload as well as probability of occurrence. An example is given for an eleven bus system, evaluating each quantity separately. The results are compared with Monte Carlo simulation.
Method and apparatus for continuous flow injection extraction analysis
Hartenstein, Steven D.; Siemer, Darryl D.
1992-01-01
A method and apparatus for a continuous flow injection batch extraction aysis system is disclosed employing extraction of a component of a first liquid into a second liquid which is a solvent for a component of the first liquid, and is immiscible with the first liquid, and for separating the first liquid from the second liquid subsequent to extraction of the component of the first liquid.
A New Power Flow Tracing Method Based on Directed Circuit
NASA Astrophysics Data System (ADS)
Zhang, Weimin; Guo, Xiaojing; Liu, Yaonian; Ni, Defu; Wang, Lina; Jia, Yanbing
In this paper, a power-sharing principle is proposed, base on the generation of the directed path. The principle is used to calculate the contributions of individual generators and loads to line flows and the real power transfer among individual generators and loads that are significant to transmission open access. From the IEEE 14-bus power systems, the effectiveness and availability of the method are verified.
Mathematical aspects of finite element methods for incompressible viscous flows
NASA Technical Reports Server (NTRS)
Gunzburger, M. D.
1986-01-01
Mathematical aspects of finite element methods are surveyed for incompressible viscous flows, concentrating on the steady primitive variable formulation. The discretization of a weak formulation of the Navier-Stokes equations are addressed, then the stability condition is considered, the satisfaction of which insures the stability of the approximation. Specific choices of finite element spaces for the velocity and pressure are then discussed. Finally, the connection between different weak formulations and a variety of boundary conditions is explored.
High speed inviscid compressible flow by the finite element method
NASA Technical Reports Server (NTRS)
Zienkiewicz, O. C.; Loehner, R.; Morgan, K.
1984-01-01
The finite element method and an explicit time stepping algorithm which is based on Taylor-Galerkin schemes with an appropriate artificial viscosity is combined with an automatic mesh refinement process which is designed to produce accurate steady state solutions to problems of inviscid compressible flow in two dimensions. The results of two test problems are included which demonstrate the excellent performance characteristics of the proposed procedures.
Reticulocyte count using thiazole orange. A flow cytometry method.
Van Hove, L; Goossens, W; Van Duppen, V; Verwilghen, R L
1990-01-01
Recently flow cytometry techniques have been developed to replace the microscope reticulocyte count. We used thiazole orange, a RNA binding fluorochrome, to discriminate reticulocytes from mature erythrocytes. Thiazole orange and the Retic-COUNT software package were evaluated for performance of routine analysis on different flow instruments. The applied methodology analysed 10(4) cells semi-automatically in an easily performed manner. Consistent results were obtained with dipotassium EDTA anticoagulated blood (stable for 30 h after venesection), with incubation times in thiazole orange solution ranging from 2 to 7 h at 25 degrees C. This allowed flexibility in specimen collection and storage and assay performance with no change in results. Changes of incubation temperature up to 30 degrees C had no measurable effect. The values obtained showed good linearity, precision and accuracy for normal, low and high reticulocyte counts. However interferences were observed: RBC autofluorescence, nucleated RBC, Howell-Jolly bodies, high leucocyte count, high platelet count and giant platelets, all falsely increased the number of reticulocytes. These artifacts were eliminated by software gate corrections, thus leaving less than 5% of the specimen to be reanalysed by the microscopic method. The thiazole orange flow cytometric method was determined to be a fast, reliable method for the routine clinical quantitation of reticulocytes.
Newton like: Minimal residual methods applied to transonic flow calculations
NASA Technical Reports Server (NTRS)
Wong, Y. S.
1984-01-01
A computational technique for the solution of the full potential equation is presented. The method consists of outer and inner iterations. The outer iterate is based on a Newton like algorithm, and a preconditioned Minimal Residual method is used to seek an approximate solution of the system of linear equations arising at each inner iterate. The present iterative scheme is formulated so that the uncertainties and difficulties associated with many iterative techniques, namely the requirements of acceleration parameters and the treatment of additional boundary conditions for the intermediate variables, are eliminated. Numerical experiments based on the new method for transonic potential flows around the NACA 0012 airfoil at different Mach numbers and different angles of attack are presented, and these results are compared with those obtained by the Approximate Factorization technique. Extention to three dimensional flow calculations and application in finite element methods for fluid dynamics problems by the present method are also discussed. The Inexact Newton like method produces a smoother reduction in the residual norm, and the number of supersonic points and circulations are rapidly established as the number of iterations is increased.
Global convergence of inexact Newton methods for transonic flow
NASA Technical Reports Server (NTRS)
Young, David P.; Melvin, Robin G.; Bieterman, Michael B.; Johnson, Forrester T.; Samant, Satish S.
1990-01-01
In computational fluid dynamics, nonlinear differential equations are essential to represent important effects such as shock waves in transonic flow. Discretized versions of these nonlinear equations are solved using iterative methods. In this paper an inexact Newton method using the GMRES algorithm of Saad and Schultz is examined in the context of the full potential equation of aerodynamics. In this setting, reliable and efficient convergence of Newton methods is difficult to achieve. A poor initial solution guess often leads to divergence or very slow convergence. This paper examines several possible solutions to these problems, including a standard local damping strategy for Newton's method and two continuation methods, one of which utilizes interpolation from a coarse grid solution to obtain the initial guess on a finer grid. It is shown that the continuation methods can be used to augment the local damping strategy to achieve convergence for difficult transonic flow problems. These include simple wings with shock waves as well as problems involving engine power effects. These latter cases are modeled using the assumption that each exhaust plume is isentropic but has a different total pressure and/or temperature than the freestream.
Path planning in uncertain flow fields using ensemble method
NASA Astrophysics Data System (ADS)
Wang, Tong; Le Maître, Olivier P.; Hoteit, Ibrahim; Knio, Omar M.
2016-08-01
An ensemble-based approach is developed to conduct optimal path planning in unsteady ocean currents under uncertainty. We focus our attention on two-dimensional steady and unsteady uncertain flows, and adopt a sampling methodology that is well suited to operational forecasts, where an ensemble of deterministic predictions is used to model and quantify uncertainty. In an operational setting, much about dynamics, topography, and forcing of the ocean environment is uncertain. To address this uncertainty, the flow field is parametrized using a finite number of independent canonical random variables with known densities, and the ensemble is generated by sampling these variables. For each of the resulting realizations of the uncertain current field, we predict the path that minimizes the travel time by solving a boundary value problem (BVP), based on the Pontryagin maximum principle. A family of backward-in-time trajectories starting at the end position is used to generate suitable initial values for the BVP solver. This allows us to examine and analyze the performance of the sampling strategy and to develop insight into extensions dealing with general circulation ocean models. In particular, the ensemble method enables us to perform a statistical analysis of travel times and consequently develop a path planning approach that accounts for these statistics. The proposed methodology is tested for a number of scenarios. We first validate our algorithms by reproducing simple canonical solutions, and then demonstrate our approach in more complex flow fields, including idealized, steady and unsteady double-gyre flows.
Path planning in uncertain flow fields using ensemble method
NASA Astrophysics Data System (ADS)
Wang, Tong; Le Maître, Olivier P.; Hoteit, Ibrahim; Knio, Omar M.
2016-10-01
An ensemble-based approach is developed to conduct optimal path planning in unsteady ocean currents under uncertainty. We focus our attention on two-dimensional steady and unsteady uncertain flows, and adopt a sampling methodology that is well suited to operational forecasts, where an ensemble of deterministic predictions is used to model and quantify uncertainty. In an operational setting, much about dynamics, topography, and forcing of the ocean environment is uncertain. To address this uncertainty, the flow field is parametrized using a finite number of independent canonical random variables with known densities, and the ensemble is generated by sampling these variables. For each of the resulting realizations of the uncertain current field, we predict the path that minimizes the travel time by solving a boundary value problem (BVP), based on the Pontryagin maximum principle. A family of backward-in-time trajectories starting at the end position is used to generate suitable initial values for the BVP solver. This allows us to examine and analyze the performance of the sampling strategy and to develop insight into extensions dealing with general circulation ocean models. In particular, the ensemble method enables us to perform a statistical analysis of travel times and consequently develop a path planning approach that accounts for these statistics. The proposed methodology is tested for a number of scenarios. We first validate our algorithms by reproducing simple canonical solutions, and then demonstrate our approach in more complex flow fields, including idealized, steady and unsteady double-gyre flows.
Selective flow path alpha particle detector and method of use
Orr, Christopher Henry; Luff, Craig Janson; Dockray, Thomas; Macarthur, Duncan Whittemore
2002-01-01
A method and apparatus for monitoring alpha contamination are provided in which ions generated in the air surrounding the item, by the passage of alpha particles, are moved to a distant detector location. The parts of the item from which ions are withdrawn can be controlled by restricting the air flow over different portions of the apparatus. In this way, detection of internal and external surfaces separately, for instance, can be provided. The apparatus and method are particularly suited for use in undertaking alpha contamination measurements during the commissioning operations.
Finite element methods for non-Newtonian flows
Gartling, D.K.
1992-10-01
The application of the finite element method to problems in non-Newtonian fluid mechanics is described. The formulation of the basic equations is presented for both inelastic and viscoelastic constitutive models. Solution algorithms for treating the material nonlinearities associated with inelastic fluids are described; typical solution procedures for the implicit stress-rate equations of viscoelastic fluids are also presented. Methods for the simulation of various types of free-surface flows are also outlined. Simple example analyses are included for both types of fluid models.
Numerical Method for Darcy Flow Derived Using Discrete Exterior Calculus
NASA Astrophysics Data System (ADS)
Hirani, A. N.; Nakshatrala, K. B.; Chaudhry, J. H.
2015-05-01
We derive a numerical method for Darcy flow, and also for Poisson's equation in mixed (first order) form, based on discrete exterior calculus (DEC). Exterior calculus is a generalization of vector calculus to smooth manifolds and DEC is one of its discretizations on simplicial complexes such as triangle and tetrahedral meshes. DEC is a coordinate invariant discretization, in that it does not depend on the embedding of the simplices or the whole mesh. We start by rewriting the governing equations of Darcy flow using the language of exterior calculus. This yields a formulation in terms of flux differential form and pressure. The numerical method is then derived by using the framework provided by DEC for discretizing differential forms and operators that act on forms. We also develop a discretization for a spatially dependent Hodge star that varies with the permeability of the medium. This also allows us to address discontinuous permeability. The matrix representation for our discrete non-homogeneous Hodge star is diagonal, with positive diagonal entries. The resulting linear system of equations for flux and pressure are saddle type, with a diagonal matrix as the top left block. The performance of the proposed numerical method is illustrated on many standard test problems. These include patch tests in two and three dimensions, comparison with analytically known solutions in two dimensions, layered medium with alternating permeability values, and a test with a change in permeability along the flow direction. We also show numerical evidence of convergence of the flux and the pressure. A convergence experiment is included for Darcy flow on a surface. A short introduction to the relevant parts of smooth and discrete exterior calculus is included in this article. We also include a discussion of the boundary condition in terms of exterior calculus.
A power flow method for evaluating vibration from underground railways
NASA Astrophysics Data System (ADS)
Hussein, M. F. M.; Hunt, H. E. M.
2006-06-01
One of the major sources of ground-borne vibration is the running of trains in underground railway tunnels. Vibration is generated at the wheel-rail interface, from where it propagates through the tunnel and surrounding soil into nearby buildings. An understanding of the dynamic interfaces between track, tunnel and soil is essential before engineering solutions to the vibration problem can be found. A new method has been developed to evaluate the effectiveness of vibration countermeasures. The method is based on calculating the mean power flow from the tunnel, paying attention to that part of the power which radiates upwards to places where buildings' foundations are expected to be found. The mean power is calculated for an infinite train moving through the tunnel with a constant velocity. An elegant mathematical expression for the mean power flow is derived, which can be used with any underground-tunnel model. To evaluate the effect of vibration countermeasures and track properties on power flow, a comprehensive three-dimensional analytical model is used. It consists of Euler-Bernoulli beams to account for the rails and the track slab. These are coupled in the wavenumber-frequency domain to a thin shell representing the tunnel embedded within an infinite continuum, with a cylindrical cavity representing the surrounding soil.
NASA Technical Reports Server (NTRS)
Mcfarland, E. R.
1981-01-01
A solution method was developed for calculating compressible inviscid flow through a linear cascade of arbitrary blade shapes. The method uses advanced surface singularity formulations which were adapted from those in current external flow analyses. The resulting solution technique provides a fast flexible calculation for flows through turbomachinery blade rows. The solution method and some examples of the method's capabilities are presented.
Method and apparatus for detecting laminar flow separation and reattachment
NASA Technical Reports Server (NTRS)
Stack, John P. (Inventor); Mangalam, Sivaramakrishnan M. (Inventor)
1989-01-01
The invention is a method and apparatus for detecting laminar flow separation and flow reattachment of a fluid stream by simultaneously sensing and comparing a plurality of output signals, each representing the dynamic shear stress at one of an equal number of sensors spaced along a straight line on the surface of an airfoil or the like that extends parallel to the fluid stream. The output signals are concurrently compared to detect the sensors across which a reversal in phase of said output signal occurs, said detected sensors being in the region of laminar separation or reattachment. The novelty in this invention is the discovery and use of the phase reversal phenomena to detect laminar separation and attachment of a fluid stream from any surface such as an airfoil supported therein.
Deformation-phase measurement by optical flow method
NASA Astrophysics Data System (ADS)
Zhao, Ran; Sun, Ping
2016-07-01
A novel algorithm which extract the out-of-plane component of deformation-phase from two continuous fringe patterns is proposed. The whole-field out-of-plane component of deformation-phase map is obtained by the estimations of the optical flow velocity field between two images and the local frequency of the original image. In this paper, the proposed algorithm is introduced and applied to simulated and experimental interferograms. Simulation and experimental results show that the new method can demodulate the out-of-plane component of deformation-phase from the visible optical flow velocity field without the operation of phase unwrapping. Further, the proposed algorithm provides a new approach for whole-field deformation-phase measurement and dynamic deformation measurement.
A nearly real-time UAV video flow mosaic method
NASA Astrophysics Data System (ADS)
Zheng, H.; Jiang, C.; Sun, M.; Li, X. D.; Xiang, R.; Liu, Lei
2014-12-01
In order to solve the problem of low accuracy and high computation cost of current video mosaic methods, and also to acquire large field of view images by the unmanned aerial vehicles (UAV), which have high accuracy and high resolution, this paper propose a method for near real-time mosaic of video flow, so that we can provide essential reference data for the earthquake relief, as well as post-disaster reconstruction and recovery, in time. In this method, we obtain the flight area scope in the route planning process, and calculate the sizes of each frame with sensor sizes and altitudes. Given an overlap degree, time intervals are calculated, and key frames are extracted. After that, feature points are detected in each frame, and they are matched using Hamming distance. The RANSAC algorithm is then applied to remove error matching and calculate parameters of the transformation model. In one-strip case, the newly extracted frame is taken as the reference image in the first half, while after the middle frame is extracted, it is the reference one until the end. Experimental results show that our method can reduce the cascading error, and improve the accuracy and quality of the mosaic images, near real-time mosaic of aerial video flow is feasible.
A diffusive information preservation method for small Knudsen number flows
NASA Astrophysics Data System (ADS)
Fei, Fei; Fan, Jing
2013-06-01
The direct simulation Monte Carlo (DSMC) method is a powerful particle-based method for modeling gas flows. It works well for relatively large Knudsen (Kn) numbers, typically larger than 0.01, but quickly becomes computationally intensive as Kn decreases due to its time step and cell size limitations. An alternative approach was proposed to relax or remove these limitations, based on replacing pairwise collisions with a stochastic model corresponding to the Fokker-Planck equation [J. Comput. Phys., 229, 1077 (2010); J. Fluid Mech., 680, 574 (2011)]. Similar to the DSMC method, the downside of that approach suffers from computationally statistical noise. To solve the problem, a diffusion-based information preservation (D-IP) method has been developed. The main idea is to track the motion of a simulated molecule from the diffusive standpoint, and obtain the flow velocity and temperature through sampling and averaging the IP quantities. To validate the idea and the corresponding model, several benchmark problems with Kn ˜ 10-3-10-4 have been investigated. It is shown that the IP calculations are not only accurate, but also efficient because they make possible using a time step and cell size over an order of magnitude larger than the mean collision time and mean free path, respectively.
A diffusive information preservation method for small Knudsen number flows
Fei, Fei; Fan, Jing
2013-06-15
The direct simulation Monte Carlo (DSMC) method is a powerful particle-based method for modeling gas flows. It works well for relatively large Knudsen (Kn) numbers, typically larger than 0.01, but quickly becomes computationally intensive as Kn decreases due to its time step and cell size limitations. An alternative approach was proposed to relax or remove these limitations, based on replacing pairwise collisions with a stochastic model corresponding to the Fokker–Planck equation [J. Comput. Phys., 229, 1077 (2010); J. Fluid Mech., 680, 574 (2011)]. Similar to the DSMC method, the downside of that approach suffers from computationally statistical noise. To solve the problem, a diffusion-based information preservation (D-IP) method has been developed. The main idea is to track the motion of a simulated molecule from the diffusive standpoint, and obtain the flow velocity and temperature through sampling and averaging the IP quantities. To validate the idea and the corresponding model, several benchmark problems with Kn ∼ 10{sup −3}–10{sup −4} have been investigated. It is shown that the IP calculations are not only accurate, but also efficient because they make possible using a time step and cell size over an order of magnitude larger than the mean collision time and mean free path, respectively.
Logically rectangular mixed methods for Darcy flow on general geometry
Arbogast, T.; Keenan, P.T.; Wheeler, M.F.; Yotov, I.
1995-12-31
The authors consider an expanded mixed finite element formulation (cell centered finite difference) for Darcy flow with a tensor absolute permeability. The reservoir can be geometrically general with internal features, but the computational domain is rectangular. The method is defined on a curvilinear grid that need not be orthogonal, obtained by mapping the rectangular, computational grid. The original flow problem becomes a similar problem with a modified permeability on the computational grid. Quadrature rules turn the mixed method into a cell-centered finite difference method with a 9 point stencil in 2-D and 19 in 3-D. As shown by theory and experiment, if the modified permeability on the computational domain is smooth, then the convergence rate is optimal and both pressure and velocity are superconvergent at certain points. If not, Lagrange multiplier pressures can be introduced on boundaries of elements so that optimal convergence is retained. This modification presents only small changes in the solution process; in fact, the same parallel domain decomposition algorithms can be applied with little or no change to the code if the modified permeability is smooth over the subdomains. This Lagrange multiplier procedure can be used to extend the difference scheme to multi-block domains, and to give a coupling with unstructured grids. In all cases, the mixed formulation is locally conservative. Computational results illustrate the advantage and convergence of this method.
Methods for blood flow measurements using ultrasound contrast agents
NASA Astrophysics Data System (ADS)
Fowlkes, J. Brian
2003-10-01
Blood flow measurements using ultrasound contrast agents are being investigated for myocardial perfusion and more recently in other organ systems. The methods are based largely on the relative increase in echogenicity due to the concentration of bubbles present in the ultrasound beam. In the simplest form, regional differences in blood volume can be inferred but the possibility exists to extract perfusion from the transit of contrast agent through tissue. Perfusion measurements rely on determining the flux of blood through a tissue volume and as such require knowledge of the fractional blood volume (FBV), i.e., ml blood/g tissue and the rate of exchange, commonly measured as the mean transit time (MTT). This presentation will discuss methods of determining each of these values and their combination to estimate tissue perfusion. Underlying principles of indicator-dilution theory will be provided in the context of ultrasound contrast agents. Current methods for determining MTT will include imaging of the intravenous bolus, in-plane contrast disruption with interval and real-time contrast recovery imaging, and control of contrast agent flow using arterial disruption (contrast interruption). The advantages and limitations of the methods will be examined along with current applications. [Work supported in part by NIH.
Flow cytometric and laser scanning microscopic approaches in epigenetics research.
Szekvolgyi, Lorant; Imre, Laszlo; Minh, Doan Xuan Quang; Hegedus, Eva; Bacso, Zsolt; Szabo, Gabor
2009-01-01
Our understanding of epigenetics has been transformed in recent years by the advance of technological possibilities based primarily on a powerful tool, chromatin immunoprecipitation (ChIP). However, in many cases, the detection of epigenetic changes requires methods providing a high-throughput (HTP) platform. Cytometry has opened a novel approach for the quantitative measurement of molecules, including PCR products, anchored to appropriately addressed microbeads (Pataki et al. 2005. Cytometry 68, 45-52). Here we show selected examples for the utility of two different cytometry-based platforms of epigenetic analysis: ChIP-on-beads, a flow-cytometric test of local histone modifications (Szekvolgyi et al. 2006. Cytometry 69, 1086-1091), and the laser scanning cytometry-based measurement of global epigenetic modifications that might help predict clinical behavior in different pathological conditions. We anticipate that such alternative tools may shortly become indispensable in clinical practice, translating the systematic screening of epigenetic tags from basic research into routine diagnostics of HTP demand.
An Engineering Aerodynamic Heating Method for Hypersonic Flow
NASA Technical Reports Server (NTRS)
Riley, Christopher J.; DeJarnette, Fred R.
1992-01-01
A capability to calculate surface heating rates has been incorporated in an approximate three-dimensional inviscid technique. Surface streamlines are calculated from the inviscid solution, and the axisymmetric analog is then used along with a set of approximate convective-heating equations to compute the surface heat transfer. The method is applied to blunted axisymmetric and three-dimensional ellipsoidal cones at angle of attack for the laminar flow of a perfect gas. The method is also applicable to turbulent and equilibrium-air conditions. The present technique predicts surface heating rates that compare favorably with experimental (ground-test and flight) data and numerical solutions of the Navier-Stokes (NS) and viscous shock-layer (VSL) equations. The new technique represents a significant improvement over current engineering aerothermal methods with only a modest increase in computational effort.
An engineering aerodynamic heating method for hypersonic flow
NASA Technical Reports Server (NTRS)
Riley, Christopher J.; Dejarnette, Fred R.
1992-01-01
A capability to calculate surface heating rates has been incorporated in an approximate three-dimensional inviscid technique. Surface streamlines are calculated from the inviscid solution, and the axisymmetric analog is then used along with a set of approximate convective-heating equations to compute the surface heat transfer. The method is applied to blunted axisymmetric and three-dimensional ellipsoidal cones at angle of attack for the laminar flow of a perfect gas. The method is also applicable to turbulent and equilibrium-air conditions. The present technique predicts surface heating rates that compare favorably with experimental (ground-test and flight) data and numerical solutions of the Navier-Stokes (NS) and viscous shock-layer (VSL) equations. The new technique represents a significant improvement over current engineering aerothermal methods with only a modest increase in computational effort.
Application of optical flow method for imaging diagnostic in JET
NASA Astrophysics Data System (ADS)
Craciunescu, T.; Murari, A.; Alonso, A.; Lang, P. T.; Kocsis, G.; Tiseanu, I.; Zoita, V.; JET EFDA Contributors
2010-05-01
An optical flow method is applied to the study of several fusion plasma relevant issues, including plasma wall interactions. A multi-resolution coarse-to-fine procedure is used in order to cope with large displacements of objects between consecutive frames, characteristic of plasma images captured by JET fast visible camera. Occlusion modeling is also implemented. The method is able to provide good results for JET fast visible camera images which can be affected by saturation, discontinuous movement, reshaping of image objects, low gray-level in-depth resolution. Significant experimental cases concerning pellet injection, plasma filaments and MARFEs are analysed. The method is able to provide the real velocity for objects moving close to structures.
Biochemical oxygen demand measurement by mediator method in flow system.
Liu, Ling; Bai, Lu; Yu, Dengbin; Zhai, Junfeng; Dong, Shaojun
2015-06-01
Using mediator as electron acceptor for biochemical oxygen demand (BOD) measurement was developed in the last decade (BODMed). However, until now, no BOD(Med) in a flow system has been reported. This work for the first time describes a flow system of BOD(Med) method (BOD(Med)-FS) by using potassium ferricyanide as mediator and carbon fiber felt as substrate material for microbial immobilization. The system can determine the BOD value within 30 min and possesses a wider analytical linear range for measuring glucose-glutamic acid (GGA) standard solution from 2 up to 200 mg L(-1) without the need of dilution. The analytical performance of the BOD(Med)-FS is comparable or better than that of the previously reported BOD(Med) method, especially its superior long-term stability up to 2 months under continuous operation. Moreover, the BOD(Med)-FS has same determination accuracy with the conventional BOD5 method by measuring real samples from a local wastewater treatment plant (WWTP).
NASA Astrophysics Data System (ADS)
Yang, T. T.; Ntone, F.
1981-05-01
Curved wall diffusers designed by using an inverse method of solution of potential flow theory have been shown to be both short and highly efficient. These features make this type of diffuser attractive in thrust ejector applications. In ejectors, however, the flow at the diffuser inlet is nearly a uniform shear flow. This paper presents a method used in examining the flow velocity along the diffuser wall and some of the analytical results for diffusers designed with potential flow theory and receiving a rotational flow. The inlet flow vorticity and the diffuser area ratios prescribed in the inverse solution of the irrotational flow are the parameters of the study. The geometry of a sample ejector using such a diffuser and its estimated thrust augmentation ratio are also presented.
COMPREHENSIVE METHOD OF CHARACTERISTICS MODELS FOR FLOW SIMULATION.
Lai, Chintu
1988-01-01
The use of the specified time interval (STI) numerical schemes has been popular in applying the method of characteristics (MOC) to unsteady open-channel flow problems. Studies and analyses of several variants of the STI schemes have led to the derivation of a new scheme, referred to herein as the multimode scheme, which combines implicit, temporal reachback, spatial reachback, and classical schemes into one. Three numerical models have been developed to implement the implicit and multimode schemes. Numerical analyses, numerical experiments, and field applications that verify, support, and demonstrate the enhanced model capabilities are presented.
NASA Astrophysics Data System (ADS)
Crane, R. A.; Cuthbert, M. O.; Timms, W.
2015-09-01
We present an interrupted-flow centrifugation technique to characterise preferential flow in low permeability media. The method entails a minimum of three phases: centrifuge-induced flow, no flow and centrifuge-induced flow, which may be repeated several times in order to most effectively characterise multi-rate mass transfer behaviour. In addition, the method enables accurate simulation of relevant in situ total stress conditions during flow by selecting an appropriate centrifugal force. We demonstrate the utility of the technique for characterising the hydraulic properties of smectite-clay-dominated core samples. All core samples exhibited a non-Fickian tracer breakthrough (early tracer arrival), combined with a decrease in tracer concentration immediately after each period of interrupted flow. This is indicative of dual (or multi-)porosity behaviour, with solute migration predominately via advection during induced flow, and via molecular diffusion (between the preferential flow network(s) and the low hydraulic conductivity domain) during interrupted flow. Tracer breakthrough curves were simulated using a bespoke dual porosity model with excellent agreement between the data and model output (Nash-Sutcliffe model efficiency coefficient was > 0.97 for all samples). In combination, interrupted-flow centrifuge experiments and dual porosity transport modelling are shown to be a powerful method to characterise preferential flow in low permeability media.
An Anelastic Allspeed Projection Method for GravitationallyStratified Flows
Gatti-Bono, Caroline; Colella, Phillip
2005-02-24
This paper looks at gravitationally-stratified atmospheric flows at low Mach and Froude numbers and proposes a new algorithm to solve the compressible Euler equations, in which the asymptotic limits are recovered numerically and the boundary conditions for block-structured local refinement methods are well-posed. The model is non-hydrostatic and the numerical algorithm uses a splitting to separate the fast acoustic dynamics from the slower anelastic dynamics. The acoustic waves are treated implicitly while the anelastic dynamics is treated semi-implicitly and an embedded-boundary method is used to represent mountain ranges. We present an example that verifies our asymptotic analysis and a set of results that compares very well with the classical gravity wave results presented by Durran.
Finite area method for nonlinear supersonic conical flows
NASA Technical Reports Server (NTRS)
Sritharan, S. S.; Seebass, A. R.
1983-01-01
A fully conservative numerical method for the computation of steady inviscid supersonic flow about general conical bodies at incidence is described. The procedure utilizes the potential approximation and implements a body conforming mesh generator. The conical potential is assumed to have its best linear variation inside each mesh cell; a secondary interlocking cell system is used to establish the flux balance required to conserve mass. In the supersonic regions the scheme is symmetrized by adding artificial viscosity in conservation form. The algorithm is nearly an order of a magnitude faster than present Euler methods and predicts known results accurately and qualitative features such as nodal point lift off correctly. Results are compared with those of other investigators.
Method and apparatus for controlling the flow rate of mercury in a flow system
Grossman, Mark W.; Speer, Richard
1991-01-01
A method for increasing the mercury flow rate to a photochemical mercury enrichment utilizing an entrainment system comprises the steps of passing a carrier gas over a pool of mercury maintained at a first temperature T1, wherein the carrier gas entrains mercury vapor; passing said mercury vapor entrained carrier gas to a second temperature zone T2 having temperature less than T1 to condense said entrained mercury vapor, thereby producing a saturated Hg condition in the carrier gas; and passing said saturated Hg carrier gas to said photochemical enrichment reactor.
Discontinuous Galerkin method for multicomponent chemically reacting flows and combustion
NASA Astrophysics Data System (ADS)
Lv, Yu; Ihme, Matthias
2014-08-01
This paper presents the development of a discontinuous Galerkin (DG) method for application to chemically reacting flows in subsonic and supersonic regimes under the consideration of variable thermo-viscous-diffusive transport properties, detailed and stiff reaction chemistry, and shock capturing. A hybrid-flux formulation is developed for treatment of the convective fluxes, combining a conservative Riemann-solver and an extended double-flux scheme. A computationally efficient splitting scheme is proposed, in which advection and diffusion operators are solved in the weak form, and the chemically stiff substep is advanced in the strong form using a time-implicit scheme. The discretization of the viscous-diffusive transport terms follows the second form of Bassi and Rebay, and the WENO-based limiter due to Zhong and Shu is extended to multicomponent systems. Boundary conditions are developed for subsonic and supersonic flow conditions, and the algorithm is coupled to thermochemical libraries to account for detailed reaction chemistry and complex transport. The resulting DG method is applied to a series of test cases of increasing physico-chemical complexity. Beginning with one- and two-dimensional multispecies advection and shock-fluid interaction problems, computational efficiency, convergence, and conservation properties are demonstrated. This study is followed by considering a series of detonation and supersonic combustion problems to investigate the convergence-rate and the shock-capturing capability in the presence of one- and multistep reaction chemistry. The DG algorithm is then applied to diffusion-controlled deflagration problems. By examining convergence properties for polynomial order and spatial resolution, and comparing these with second-order finite-volume solutions, it is shown that optimal convergence is achieved and that polynomial refinement provides advantages in better resolving the localized flame structure and complex flow-field features
Bluff Body Flow Simulation Using a Vortex Element Method
Anthony Leonard; Phillippe Chatelain; Michael Rebel
2004-09-30
Heavy ground vehicles, especially those involved in long-haul freight transportation, consume a significant part of our nation's energy supply. it is therefore of utmost importance to improve their efficiency, both to reduce emissions and to decrease reliance on imported oil. At highway speeds, more than half of the power consumed by a typical semi truck goes into overcoming aerodynamic drag, a fraction which increases with speed and crosswind. Thanks to better tools and increased awareness, recent years have seen substantial aerodynamic improvements by the truck industry, such as tractor/trailer height matching, radiator area reduction, and swept fairings. However, there remains substantial room for improvement as understanding of turbulent fluid dynamics grows. The group's research effort focused on vortex particle methods, a novel approach for computational fluid dynamics (CFD). Where common CFD methods solve or model the Navier-Stokes equations on a grid which stretches from the truck surface outward, vortex particle methods solve the vorticity equation on a Lagrangian basis of smooth particles and do not require a grid. They worked to advance the state of the art in vortex particle methods, improving their ability to handle the complicated, high Reynolds number flow around heavy vehicles. Specific challenges that they have addressed include finding strategies to accurate capture vorticity generation and resultant forces at the truck wall, handling the aerodynamics of spinning bodies such as tires, application of the method to the GTS model, computation time reduction through improved integration methods, a closest point transform for particle method in complex geometrics, and work on large eddy simulation (LES) turbulence modeling.
Numerical method of characteristics for one-dimensional blood flow
NASA Astrophysics Data System (ADS)
Acosta, Sebastian; Puelz, Charles; Rivière, Béatrice; Penny, Daniel J.; Rusin, Craig G.
2015-08-01
Mathematical modeling at the level of the full cardiovascular system requires the numerical approximation of solutions to a one-dimensional nonlinear hyperbolic system describing flow in a single vessel. This model is often simulated by computationally intensive methods like finite elements and discontinuous Galerkin, while some recent applications require more efficient approaches (e.g. for real-time clinical decision support, phenomena occurring over multiple cardiac cycles, iterative solutions to optimization/inverse problems, and uncertainty quantification). Further, the high speed of pressure waves in blood vessels greatly restricts the time step needed for stability in explicit schemes. We address both cost and stability by presenting an efficient and unconditionally stable method for approximating solutions to diagonal nonlinear hyperbolic systems. Theoretical analysis of the algorithm is given along with a comparison of our method to a discontinuous Galerkin implementation. Lastly, we demonstrate the utility of the proposed method by implementing it on small and large arterial networks of vessels whose elastic and geometrical parameters are physiologically relevant.
Domain decomposition methods for the parallel computation of reacting flows
NASA Technical Reports Server (NTRS)
Keyes, David E.
1988-01-01
Domain decomposition is a natural route to parallel computing for partial differential equation solvers. Subdomains of which the original domain of definition is comprised are assigned to independent processors at the price of periodic coordination between processors to compute global parameters and maintain the requisite degree of continuity of the solution at the subdomain interfaces. In the domain-decomposed solution of steady multidimensional systems of PDEs by finite difference methods using a pseudo-transient version of Newton iteration, the only portion of the computation which generally stands in the way of efficient parallelization is the solution of the large, sparse linear systems arising at each Newton step. For some Jacobian matrices drawn from an actual two-dimensional reacting flow problem, comparisons are made between relaxation-based linear solvers and also preconditioned iterative methods of Conjugate Gradient and Chebyshev type, focusing attention on both iteration count and global inner product count. The generalized minimum residual method with block-ILU preconditioning is judged the best serial method among those considered, and parallel numerical experiments on the Encore Multimax demonstrate for it approximately 10-fold speedup on 16 processors.
Finite volume methods for submarine debris flows and generated waves
NASA Astrophysics Data System (ADS)
Kim, Jihwan; Løvholt, Finn; Issler, Dieter
2016-04-01
Submarine landslides can impose great danger to the underwater structures and generate destructive tsunamis. Submarine debris flows often behave like visco-plastic materials, and the Herschel-Bulkley rheological model is known to be appropriate for describing the motion. In this work, we develop numerical schemes for the visco-plastic debris flows using finite volume methods in Eulerian coordinates with two horizontal dimensions. We provide parameter sensitivity analysis and demonstrate how common ad-hoc assumptions such as including a minimum shear layer depth influence the modeling of the landslide dynamics. Hydrodynamic resistance forces, hydroplaning, and remolding are all crucial terms for underwater landslides, and are hence added into the numerical formulation. The landslide deformation is coupled to the water column and simulated in the Clawpack framework. For the propagation of the tsunamis, the shallow water equations and the Boussinesq-type equations are employed to observe how important the wave dispersion is. Finally, two cases in central Norway, i.e. the subaerial quick clay landslide at Byneset in 2012, and the submerged tsunamigenic Statland landslide in 2014, are both presented for validation. The research leading to these results has received funding from the Research Council of Norway under grant number 231252 (Project TsunamiLand) and the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement 603839 (Project ASTARTE).
Flow Diode and Method for Controlling Fluid Flow Origin of the Invention
NASA Technical Reports Server (NTRS)
Dyson, Rodger W (Inventor)
2015-01-01
A flow diode configured to permit fluid flow in a first direction while preventing fluid flow in a second direction opposite the first direction is disclosed. The flow diode prevents fluid flow without use of mechanical closures or moving parts. The flow diode utilizes a bypass flowline whereby all fluid flow in the second direction moves into the bypass flowline having a plurality of tortuous portions providing high fluidic resistance. The portions decrease in diameter such that debris in the fluid is trapped. As fluid only travels in one direction through the portions, the debris remains trapped in the portions.
Valuing instream flows using the hedonic price method
NASA Astrophysics Data System (ADS)
Netusil, Noelwah R.; Summers, Matthew T.
2009-11-01
The Oregon Water Trust (OWT) uses a market-based approach to protect and enhance instream flows in Oregon. We use the hedonic price method to estimate the effect of numerous variables on the annualized price OWT pays for water rights: the amount of water protected by the transaction, transaction type (state approved or contractual agreement), presence of anadromous and/or resident fish, and if a fish is listed under the Endangered Species Act (ESA). We find evidence of a premium for state-approved transactions and for transactions that protect water in streams with listed species. Adjusting the amount of water protected by each transaction to include only rights that will be delivered with a high degree of certainty produces coefficient estimates that are similar, but more accurate, than using unadjusted water rights amounts.
Design of a Variational Multiscale Method for Turbulent Compressible Flows
NASA Technical Reports Server (NTRS)
Diosady, Laslo Tibor; Murman, Scott M.
2013-01-01
A spectral-element framework is presented for the simulation of subsonic compressible high-Reynolds-number flows. The focus of the work is maximizing the efficiency of the computational schemes to enable unsteady simulations with a large number of spatial and temporal degrees of freedom. A collocation scheme is combined with optimized computational kernels to provide a residual evaluation with computational cost independent of order of accuracy up to 16th order. The optimized residual routines are used to develop a low-memory implicit scheme based on a matrix-free Newton-Krylov method. A preconditioner based on the finite-difference diagonalized ADI scheme is developed which maintains the low memory of the matrix-free implicit solver, while providing improved convergence properties. Emphasis on low memory usage throughout the solver development is leveraged to implement a coupled space-time DG solver which may offer further efficiency gains through adaptivity in both space and time.
Methods for estimating flow-duration and annual mean-flow statistics for ungaged streams in Oklahoma
Esralew, Rachel A.; Smith, S. Jerrod
2010-01-01
Flow statistics can be used to provide decision makers with surface-water information needed for activities such as water-supply permitting, flow regulation, and other water rights issues. Flow statistics could be needed at any location along a stream. Most often, streamflow statistics are needed at ungaged sites, where no flow data are available to compute the statistics. Methods are presented in this report for estimating flow-duration and annual mean-flow statistics for ungaged streams in Oklahoma. Flow statistics included the (1) annual (period of record), (2) seasonal (summer-autumn and winter-spring), and (3) 12 monthly duration statistics, including the 20th, 50th, 80th, 90th, and 95th percentile flow exceedances, and the annual mean-flow (mean of daily flows for the period of record). Flow statistics were calculated from daily streamflow information collected from 235 streamflow-gaging stations throughout Oklahoma and areas in adjacent states. A drainage-area ratio method is the preferred method for estimating flow statistics at an ungaged location that is on a stream near a gage. The method generally is reliable only if the drainage-area ratio of the two sites is between 0.5 and 1.5. Regression equations that relate flow statistics to drainage-basin characteristics were developed for the purpose of estimating selected flow-duration and annual mean-flow statistics for ungaged streams that are not near gaging stations on the same stream. Regression equations were developed from flow statistics and drainage-basin characteristics for 113 unregulated gaging stations. Separate regression equations were developed by using U.S. Geological Survey streamflow-gaging stations in regions with similar drainage-basin characteristics. These equations can increase the accuracy of regression equations used for estimating flow-duration and annual mean-flow statistics at ungaged stream locations in Oklahoma. Streamflow-gaging stations were grouped by selected drainage
A general method to determine the stability of compressible flows
NASA Technical Reports Server (NTRS)
Guenther, R. A.; Chang, I. D.
1982-01-01
Several problems were studied using two completely different approaches. The initial method was to use the standard linearized perturbation theory by finding the value of the individual small disturbance quantities based on the equations of motion. These were serially eliminated from the equations of motion to derive a single equation that governs the stability of fluid dynamic system. These equations could not be reduced unless the steady state variable depends only on one coordinate. The stability equation based on one dependent variable was found and was examined to determine the stability of a compressible swirling jet. The second method applied a Lagrangian approach to the problem. Since the equations developed were based on different assumptions, the condition of stability was compared only for the Rayleigh problem of a swirling flow, both examples reduce to the Rayleigh criterion. This technique allows including the viscous shear terms which is not possible in the first method. The same problem was then examined to see what effect shear has on stability.
A Method of Detecting Fire Smoke by Using Optical Flow
NASA Astrophysics Data System (ADS)
Terada, Kenji; Miyahara, Hiroyuki; Nii, Yasutoshi
In this paper, the authors propose a method for detecting fire smoke by using the optical flow. This method is not influenced against the image obtainment environment. About 60,000 fires have occurred every year in Japan. To be most important to the fires is an early period fire fighting. At present, the automatic devices of detectiong fires is needed. The alarms which can detect smoke and heat are utilized to house fires. However, these alarms are not useful for the outside of house such as the incendiary or woodland fire. This method is able to detect such a flame that becomes a fire is the early period. First, the region of the flame in the images obtained from the observation camera is detected. Next, the characteristic quantity that expresses the smoke is extracted. This characteristic is not influenced to the motion such as the cloud, leaf and moving objects. In other words, the only smoke can be detected, from the range which looks like the flame in the image.
A proposed through-flow inverse method for the design of mixed-flow pumps
NASA Technical Reports Server (NTRS)
Borges, Joao Eduardo
1991-01-01
A through-flow (hub-to-shroud) truly inverse method is proposed and described. It uses an imposition of mean swirl, i.e., radius times mean tangential velocity, given throughout the meridional section of the turbomachine as an initial design specification. In the present implementation, it is assumed that the fluid is inviscid, incompressible, and irrotational at inlet and that the blades are supposed to have zero thickness. Only blade rows that impart to the fluid a constant work along the space are considered. An application of this procedure to design the rotor of a mixed-flow pump is described in detail. The strategy used to find a suitable mean swirl distribution and the other design inputs is also described. The final blade shape and pressure distributions on the blade surface are presented, showing that it is possible to obtain feasible designs using this technique. Another advantage of this technique is the fact that it does not require large amounts of CPU time.
Consistent lattice Boltzmann methods for incompressible axisymmetric flows.
Zhang, Liangqi; Yang, Shiliang; Zeng, Zhong; Yin, Linmao; Zhao, Ya; Chew, Jia Wei
2016-08-01
In this work, consistent lattice Boltzmann (LB) methods for incompressible axisymmetric flows are developed based on two efficient axisymmetric LB models available in the literature. In accord with their respective original models, the proposed axisymmetric models evolve within the framework of the standard LB method and the source terms contain no gradient calculations. Moreover, the incompressibility conditions are realized with the Hermite expansion, thus the compressibility errors arising in the existing models are expected to be reduced by the proposed incompressible models. In addition, an extra relaxation parameter is added to the Bhatnagar-Gross-Krook collision operator to suppress the effect of the ghost variable and thus the numerical stability of the present models is significantly improved. Theoretical analyses, based on the Chapman-Enskog expansion and the equivalent moment system, are performed to derive the macroscopic equations from the LB models and the resulting truncation terms (i.e., the compressibility errors) are investigated. In addition, numerical validations are carried out based on four well-acknowledged benchmark tests and the accuracy and applicability of the proposed incompressible axisymmetric LB models are verified. PMID:27627407
Consistent lattice Boltzmann methods for incompressible axisymmetric flows
NASA Astrophysics Data System (ADS)
Zhang, Liangqi; Yang, Shiliang; Zeng, Zhong; Yin, Linmao; Zhao, Ya; Chew, Jia Wei
2016-08-01
In this work, consistent lattice Boltzmann (LB) methods for incompressible axisymmetric flows are developed based on two efficient axisymmetric LB models available in the literature. In accord with their respective original models, the proposed axisymmetric models evolve within the framework of the standard LB method and the source terms contain no gradient calculations. Moreover, the incompressibility conditions are realized with the Hermite expansion, thus the compressibility errors arising in the existing models are expected to be reduced by the proposed incompressible models. In addition, an extra relaxation parameter is added to the Bhatnagar-Gross-Krook collision operator to suppress the effect of the ghost variable and thus the numerical stability of the present models is significantly improved. Theoretical analyses, based on the Chapman-Enskog expansion and the equivalent moment system, are performed to derive the macroscopic equations from the LB models and the resulting truncation terms (i.e., the compressibility errors) are investigated. In addition, numerical validations are carried out based on four well-acknowledged benchmark tests and the accuracy and applicability of the proposed incompressible axisymmetric LB models are verified.
Devices and methods of operation thereof for providing stable flow for centrifugal compressors
NASA Technical Reports Server (NTRS)
Skoch, Gary J. (Inventor); Stevens, Mark A. (Inventor); Jett, Thomas A. (Inventor)
2008-01-01
Centrifugal compressor flow stabilizing devices and methods of operation thereof are disclosed that act upon the flow field discharging from the impeller of a centrifugal compressor and modify the flow field ahead of the diffuser vanes such that flow conditions contributing to rotating stall and surge are reduced or even eliminated. In some embodiments, shaped rods and methods of operation thereof are disclosed, whereas in other embodiments reverse-tangent air injection devices and methods are disclosed.
Analysis of methods to estimate spring flows in a karst aquifer
Sepulveda, N.
2009-01-01
Hydraulically and statistically based methods were analyzed to identify the most reliable method to predict spring flows in a karst aquifer. Measured water levels at nearby observation wells, measured spring pool altitudes, and the distance between observation wells and the spring pool were the parameters used to match measured spring flows. Measured spring flows at six Upper Floridan aquifer springs in central Florida were used to assess the reliability of these methods to predict spring flows. Hydraulically based methods involved the application of the Theis, Hantush-Jacob, and Darcy-Weisbach equations, whereas the statistically based methods were the multiple linear regressions and the technology of artificial neural networks (ANNs). Root mean square errors between measured and predicted spring flows using the Darcy-Weisbach method ranged between 5% and 15% of the measured flows, lower than the 7% to 27% range for the Theis or Hantush-Jacob methods. Flows at all springs were estimated to be turbulent based on the Reynolds number derived from the Darcy-Weisbach equation for conduit flow. The multiple linear regression and the Darcy-Weisbach methods had similar spring flow prediction capabilities. The ANNs provided the lowest residuals between measured and predicted spring flows, ranging from 1.6% to 5.3% of the measured flows. The model prediction efficiency criteria also indicated that the ANNs were the most accurate method predicting spring flows in a karst aquifer. ?? 2008 National Ground Water Association.
Innovative Method for Greatly Reducing Flow Resistance and Obtaining Well-Ordered Continuous Flow
NASA Astrophysics Data System (ADS)
Lin, Weiyi
2009-11-01
In this paper, firstly, the experiment on the flow resistance of the aerated pipe flow is introduced. And some experimental research on comparison between different volumes of air entrained is presented. Secondly, the characteristics of Gravity Pipe Flow under the action of Torricelli's Vacuum, shortly called as GPFUTV are dissertated, including creative and functional design, fundamental principle, etc. Under GPFUTV condition the water flow in the tube is full-pipe and continuous, colorless and non-aerated, high-speed and non-rotational as distinguished from laminar flow. Thirdly, an appeal in relation to the experimental research, the applied studies and basic theory research is given. For instance, the well-known Reynolds' experiment under GPFUTV condition, the potential for GPFUTV to be developed for deep seawater suction technology, seawater intake pipe of OTEC and lifting technology for deep ocean mining in Fe-Mn concretions, flow stability and flow resistance under GPFUTV condition, etc.
Calculation of unsteady transonic flows using the integral equation method
NASA Technical Reports Server (NTRS)
Nixon, D.
1978-01-01
The basic integral equations for a harmonically oscillating airfoil in a transonic flow with shock waves are derived; the reduced frequency is assumed to be small. The problems associated with shock wave motion are treated using a strained coordinate system. The integral equation is linear and consists of both line integrals and surface integrals over the flow field which are evaluated by quadrature. This leads to a set of linear algebraic equations that can be solved directly. The shock motion is obtained explicitly by enforcing the condition that the flow is continuous except at a shock wave. Results obtained for both lifting and nonlifting oscillatory flows agree satisfactorily with other accurate results.
Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry.
Sica, Valentina; Maiuri, M Chiara; Kroemer, Guido; Galluzzi, Lorenzo
2016-01-01
Different modes of regulated cell death (RCD) can be initiated by distinct molecular machineries and their morphological manifestations can be difficult to discriminate. Moreover, cells responding to stress often activate an adaptive response centered around autophagy, and whether such a response is cytoprotective or cytotoxic cannot be predicted based on morphological parameters only. Molecular definitions are therefore important to understand various RCD subroutines from a mechanistic perspective. In vitro, various forms of RCD including apoptosis and autophagic cell death can be easily discriminated from each other with assays that involve chemical or pharmacological interventions targeting key components of either pathway. Here, we detail a straightforward method to discriminate apoptosis from autophagic cell death by flow cytometry, based on the broad-spectrum caspase inhibitor Z-VAD-fmk and the genetic inhibition of ATG5.
Methods for the calculation of axial wave numbers in lined ducts with mean flow
NASA Technical Reports Server (NTRS)
Eversman, W.
1981-01-01
A survey is made of the methods available for the calculation of axial wave numbers in lined ducts. Rectangular and circular ducts with both uniform and non-uniform flow are considered as are ducts with peripherally varying liners. A historical perspective is provided by a discussion of the classical methods for computing attenuation when no mean flow is present. When flow is present these techniques become either impractical or impossible. A number of direct eigenvalue determination schemes which have been used when flow is present are discussed. Methods described are extensions of the classical no-flow technique, perturbation methods based on the no-flow technique, direct integration methods for solution of the eigenvalue equation, an integration-iteration method based on the governing differential equation for acoustic transmission, Galerkin methods, finite difference methods, and finite element methods.
Axial and Centrifugal Compressor Mean Line Flow Analysis Method
NASA Technical Reports Server (NTRS)
Veres, Joseph P.
2009-01-01
This paper describes a method to estimate key aerodynamic parameters of single and multistage axial and centrifugal compressors. This mean-line compressor code COMDES provides the capability of sizing single and multistage compressors quickly during the conceptual design process. Based on the compressible fluid flow equations and the Euler equation, the code can estimate rotor inlet and exit blade angles when run in the design mode. The design point rotor efficiency and stator losses are inputs to the code, and are modeled at off design. When run in the off-design analysis mode, it can be used to generate performance maps based on simple models for losses due to rotor incidence and inlet guide vane reset angle. The code can provide an improved understanding of basic aerodynamic parameters such as diffusion factor, loading levels and incidence, when matching multistage compressor blade rows at design and at part-speed operation. Rotor loading levels and relative velocity ratio are correlated to the onset of compressor surge. NASA Stage 37 and the three-stage NASA 74-A axial compressors were analyzed and the results compared to test data. The code has been used to generate the performance map for the NASA 76-B three-stage axial compressor featuring variable geometry. The compressor stages were aerodynamically matched at off-design speeds by adjusting the variable inlet guide vane and variable stator geometry angles to control the rotor diffusion factor and incidence angles.
Quadrature Moments Method for the Simulation of Turbulent Reactive Flows
NASA Technical Reports Server (NTRS)
Raman, Venkatramanan; Pitsch, Heinz; Fox, Rodney O.
2003-01-01
A sub-filter model for reactive flows, namely the DQMOM model, was formulated for Large Eddy Simulation (LES) using the filtered mass density function. Transport equations required to determine the location and size of the delta-peaks were then formulated for a 2-peak decomposition of the FDF. The DQMOM scheme was implemented in an existing structured-grid LES solver. Simulations of scalar shear layer using an experimental configuration showed that the first and second moments of both reactive and inert scalars are in good agreement with a conventional Lagrangian scheme that evolves the same FDF. Comparisons with LES simulations performed using laminar chemistry assumption for the reactive scalar show that the new method provides vast improvements at minimal computational cost. Currently, the DQMOM model is being implemented for use with the progress variable/mixture fraction model of Pierce. Comparisons with experimental results and LES simulations using a single-environment for the progress-variable are planned. Future studies will aim at understanding the effect of increase in environments on predictions.
The backward phase flow method for the Eulerian finite time Lyapunov exponent computations
Leung, Shingyu
2013-12-15
We propose a simple Eulerian approach to compute the moderate to long time flow map for approximating the Lyapunov exponent of a (periodic or aperiodic) dynamical system. The idea is to generalize a recently proposed backward phase flow method which is specially designed for long time level set propagation. Unlike the original phase flow method or the backward phase flow method, which is applicable only to autonomous systems, the current approach can also be applied to any time-dependent (periodic or aperiodic) flow. We will discuss the stability of the proposed method. Numerical examples will be given to demonstrate the effectiveness of the algorithm.
The least-squares finite element method for low-mach-number compressible viscous flows
NASA Technical Reports Server (NTRS)
Yu, Sheng-Tao
1994-01-01
The present paper reports the development of the Least-Squares Finite Element Method (LSFEM) for simulating compressible viscous flows at low Mach numbers in which the incompressible flows pose as an extreme. Conventional approach requires special treatments for low-speed flows calculations: finite difference and finite volume methods are based on the use of the staggered grid or the preconditioning technique; and, finite element methods rely on the mixed method and the operator-splitting method. In this paper, however, we show that such difficulty does not exist for the LSFEM and no special treatment is needed. The LSFEM always leads to a symmetric, positive-definite matrix through which the compressible flow equations can be effectively solved. Two numerical examples are included to demonstrate the method: first, driven cavity flows at various Reynolds numbers; and, buoyancy-driven flows with significant density variation. Both examples are calculated by using full compressible flow equations.
Method for flow cytometric monitoring of Renibacterium salmoninarum inactivation
Pascho, R.J.; Ongerth, J.E.
2000-01-01
with bacteriological culture (r2 ??? 0.22). In both assessments, there was a correlation between the estimates of inactivation based upon HRFI and CS analyses (r2 > 0.99). These results suggest that flow cytometry can be used as a supplementary or alternative method to bacteriological culture for monitoring the inactivation of R. salmoninarum.
Itzel, Gary Michael; Devine, II, Robert Henry; Chopra, Sanjay; Toornman, Thomas Nelson
2003-07-08
A coolant flow control structure is provided to channel cooling media flow to the fillet region defined at the transition between the wall of a nozzle vane and a wall of a nozzle segment, for cooling the fillet region. In an exemplary embodiment, the flow control structure defines a gap with the fillet region to achieve the required heat transfer coefficients in this region to meet part life requirements.
A numerical method for a model of two-phase flow in a coupled free flow and porous media system
NASA Astrophysics Data System (ADS)
Chen, Jie; Sun, Shuyu; Wang, Xiao-Ping
2014-07-01
In this article, we study two-phase fluid flow in coupled free flow and porous media regions. The model consists of coupled Cahn-Hilliard and Navier-Stokes equations in the free fluid region and the two-phase Darcy law in the porous medium region. We propose a Robin-Robin domain decomposition method for the coupled Navier-Stokes and Darcy system with the generalized Beavers-Joseph-Saffman condition on the interface between the free flow and the porous media regions. Numerical examples are presented to illustrate the effectiveness of this method.
A velocity-correction projection method based immersed boundary method for incompressible flows
NASA Astrophysics Data System (ADS)
Cai, Shanggui
2014-11-01
In the present work we propose a novel direct forcing immersed boundary method based on the velocity-correction projection method of [J.L. Guermond, J. Shen, Velocity-correction projection methods for incompressible flows, SIAM J. Numer. Anal., 41 (1)(2003) 112]. The principal idea of immersed boundary method is to correct the velocity in the vicinity of the immersed object by using an artificial force to mimic the presence of the physical boundaries. Therefore, velocity-correction projection method is preferred to its pressure-correction counterpart in the present work. Since the velocity-correct projection method is considered as a dual class of pressure-correction method, the proposed method here can also be interpreted in the way that first the pressure is predicted by treating the viscous term explicitly without the consideration of the immersed boundary, and the solenoidal velocity is used to determine the volume force on the Lagrangian points, then the non-slip boundary condition is enforced by correcting the velocity with the implicit viscous term. To demonstrate the efficiency and accuracy of the proposed method, several numerical simulations are performed and compared with the results in the literature. China Scholarship Council.
Piecewise uniform conduction-like flow channels and method therefor
Cummings, Eric B.; Fiechtner, Gregory J.
2006-02-28
A low-dispersion methodology for designing microfabricated conduction channels for on-chip electrokinetic-based systems is presented. The technique relies on trigonometric relations that apply for ideal electrokinetic flows, allowing faceted channels to be designed on chips using common drafting software and a hand calculator. Flows are rotated and stretched along the abrupt interface between adjacent regions with differing permeability. Regions bounded by interfaces form flow "prisms" that can be combined with other designed prisms to obtain a wide range of turning angles and expansion ratios while minimizing dispersion. Designs are demonstrated using two-dimensional numerical solutions of the Laplace equation.
Method and applications of fiber synthesis using laminar flow
NASA Astrophysics Data System (ADS)
Burns, Bradley Justin
A Laminar Flow Reactor (LFR) using the principles of hydrodynamic focusing was created and used to fabricate functional composite polymer fibers. These fibers had the ability to conduct or serve as a carrier for singlet oxygen-generating molecules. Critical to the process was designing an easy-to-fabricate, inexpensive device and developing a repeatable method that made efficient use of the materials. The initial designs used a planar layout and hydrodynamically focused in only one dimension while later versions switched to a two-fluid concentric design. Modeling was undertaken and verified for the different device layouts. Three types of conductive particles were embedded in the formed polymer: silver, indium tin oxide (ITO) and polyaniline. The polymer was also used as a carrier to two singlet oxygen generating molecules: Methylene Blue (MB) and perylene. Both were effective in killing Bacillus thuringiensis but MB leached from the fiber into the tested cell suspension. Perylene, which is not water soluble, did not leach out and was just as effective as MB. Research that was performed at ITT is also presented. A critical need exists to detect, identify, quantify, locate, and track virus and toxin aerosols to provide early warning during both light and dark conditions. The solution presented is a remote sensing technology using seeding particles. Seeding particles developed during this program provide specific identification of threat cloud content. When introduced to the threat cloud the seeders will bind specifically to the analyte of interest and upon interrogation from a stand off laser source will fluoresce. The fluorescent signal is detected from a distance using a long-range microscope and collection optics that allow detection of low concentrations of threat aerosols.
Lattice-Boltzmann method for Stokes flow of Deformable Particles
NASA Astrophysics Data System (ADS)
Wu, Jingshu; Ding, E.-Jiang; Aidun, Cyrus
2006-11-01
Predicting the rheology of deformable particles suspended in viscous fluid is important in industrial and biological transport applications. We combine a Stokes lattice-Boltzmann Model (SLBM) and Lattice Spring Model (LSM) to capture the dynamics of particles with deformable membrane with enclosed fluid. We simulate blood flow in a small vessel which has deformable arterial wall and red blood cell (RBC) membrane. We find that the flow is affected by the physical properties of wall, membrane and enclosed fluid and these are very important in the hemodynamic applications. The combination of SLBM-LSM can calculate the mesoscale blood flow close to the endothelial surface layer (ESL) or its subset, the glycocalyx layer. And it is also a strong tool to capture the multiphase flow with large number of deformable particles.
Development of a flow rate monitoring method for the wearable ventricular assist device driver.
Ohnuma, Kentaro; Homma, Akihiko; Sumikura, Hirohito; Tsukiya, Tomonori; Takewa, Yoshiaki; Mizuno, Toshihide; Mukaibayashi, Hiroshi; Kojima, Koichi; Katano, Kazuo; Taenaka, Yoshiyuki; Tatsumi, Eisuke
2015-06-01
Our research institute has been working on the development of a compact wearable drive unit for an extracorporeal ventricular assist device (VAD) with a pneumatically driven pump. A method for checking the pump blood flow on the side of the drive unit without modifying the existing blood pump and impairing the portability of it will be useful. In this study, to calculate the pump flow rate indirectly from measuring the flow rate of the driving air of the VAD air chamber, we conducted experiments using a mock circuit to investigate the correlation between the air flow rate and the pump flow rate as well as its accuracy and error factors. The pump flow rate was measured using an ultrasonic flow meter at the inflow and outflow tube, and the air flow was measured using a thermal mass flow meter at the driveline. Similarity in the instantaneous waveform was confirmed between the air flow rate in the driveline and the pump flow rate. Some limitations of this technique were indicated by consideration of the error factors. A significant correlation was found between the average pump flow rate in the ejecting direction and the average air flow rate in the ejecting direction (R2 = 0.704-0.856), and the air flow rate in the filling direction (R2 = 0.947-0.971). It was demonstrated that the average pump flow rate was estimated exactly in a wide range of drive conditions using the air flow of the filling phase. PMID:25500948
Base flow separation: A comparison of analytical and mass balance methods
NASA Astrophysics Data System (ADS)
Lott, Darline A.; Stewart, Mark T.
2016-04-01
Base flow is the ground water contribution to stream flow. Many activities, such as water resource management, calibrating hydrological and climate models, and studies of basin hydrology, require good estimates of base flow. The base flow component of stream flow is usually determined by separating a stream hydrograph into two components, base flow and runoff. Analytical methods, mathematical functions or algorithms used to calculate base flow directly from discharge, are the most widely used base flow separation methods and are often used without calibration to basin or gage-specific parameters other than basin area. In this study, six analytical methods are compared to a mass balance method, the conductivity mass-balance (CMB) method. The base flow index (BFI) values for 35 stream gages are obtained from each of the seven methods with each gage having at least two consecutive years of specific conductance data and 30 years of continuous discharge data. BFI is cumulative base flow divided by cumulative total discharge over the period of record of analysis. The BFI value is dimensionless, and always varies from 0 to 1. Areas of basins used in this study range from 27 km2 to 68,117 km2. BFI was first determined for the uncalibrated analytical methods. The parameters of each analytical method were then calibrated to produce BFI values as close to the CMB derived BFI values as possible. One of the methods, the power function (aQb + cQ) method, is inherently calibrated and was not recalibrated. The uncalibrated analytical methods have an average correlation coefficient of 0.43 when compared to CMB-derived values, and an average correlation coefficient of 0.93 when calibrated with the CMB method. Once calibrated, the analytical methods can closely reproduce the base flow values of a mass balance method. Therefore, it is recommended that analytical methods be calibrated against tracer or mass balance methods.
Lattice Boltzmann Method for 3-D Flows with Curved Boundary
NASA Technical Reports Server (NTRS)
Mei, Renwei; Shyy, Wei; Yu, Dazhi; Luo, Li-Shi
2002-01-01
In this work, we investigate two issues that are important to computational efficiency and reliability in fluid dynamics applications of the lattice, Boltzmann equation (LBE): (1) Computational stability and accuracy of different lattice Boltzmann models and (2) the treatment of the boundary conditions on curved solid boundaries and their 3-D implementations. Three athermal 3-D LBE models (D3QI5, D3Ql9, and D3Q27) are studied and compared in terms of efficiency, accuracy, and robustness. The boundary treatment recently developed by Filippova and Hanel and Met et al. in 2-D is extended to and implemented for 3-D. The convergence, stability, and computational efficiency of the 3-D LBE models with the boundary treatment for curved boundaries were tested in simulations of four 3-D flows: (1) Fully developed flows in a square duct, (2) flow in a 3-D lid-driven cavity, (3) fully developed flows in a circular pipe, and (4) a uniform flow over a sphere. We found that while the fifteen-velocity 3-D (D3Ql5) model is more prone to numerical instability and the D3Q27 is more computationally intensive, the 63Q19 model provides a balance between computational reliability and efficiency. Through numerical simulations, we demonstrated that the boundary treatment for 3-D arbitrary curved geometry has second-order accuracy and possesses satisfactory stability characteristics.
Numerical methods for simulating blood flow at macro, micro, and multi scales.
Imai, Yohsuke; Omori, Toshihiro; Shimogonya, Yuji; Yamaguchi, Takami; Ishikawa, Takuji
2016-07-26
In the past decade, numerical methods for the computational biomechanics of blood flow have progressed to overcome difficulties in diverse applications from cellular to organ scales. Such numerical methods may be classified by the type of computational mesh used for the fluid domain, into fixed mesh methods, moving mesh (boundary-fitted mesh) methods, and mesh-free methods. The type of computational mesh used is closely related to the characteristics of each method. We herein provide an overview of numerical methods recently used to simulate blood flow at macro and micro scales, with a focus on computational meshes. We also discuss recent progress in the multi-scale modeling of blood flow.
Simulation of large incompressible flows by the finite element method
NASA Astrophysics Data System (ADS)
Bercovier, M.; Engelman, M.
Super computers together with low cost 32 bit machines with graphics render more actual the challenge of designing a general purpose Fluid Dynamic code. Such an approach was the key to the introduction of computational structural mechanics into the design cycle. There FEM codes have replaced experiments and are evaluation tools at every stage. Except for a very limited domain (low Reynolds numbers for instance) this "black box" concept cannot be aplied in fluid mechanics. Nevertheless the success of the modular approach of FIDAP, the addition of new models and the extension of its pre and post-processing capabilities bring a new bridge between the fluid mechanics engineer and the design floor shop. It is now the task of the scientific community to design acceptable models in domains like turbulent flows,multiphase flows slightly compressible flows and so on. At the same time faster and robust algorithms for highly non linear problems must be devised.
Method and apparatus for adapting steady flow with cyclic thermodynamics
Swift, Gregory W.; Reid, Robert S.; Ward, William C.
2000-01-01
Energy transfer apparatus has a resonator for supporting standing acoustic waves at a selected frequency with a steady flow process fluid thermodynamic medium and a solid medium having heat capacity. The fluid medium and the solid medium are disposed within the resonator for thermal contact therebetween and for relative motion therebetween. The relative motion is produced by a first means for producing a steady velocity component and second means for producing an oscillating velocity component at the selected frequency and concomitant wavelength of the standing acoustic wave. The oscillating velocity and associated oscillating pressure component provide energy transfer between the steady flow process fluid and the solid medium as the steady flow process fluid moves through the resonator.
A New Method for Flow Rate Measurement in Millimeter-Scale Pipes
Ji, Haifeng; Gao, Xuemin; Wang, Baoliang; Huang, Zhiyao; Li, Haiqing
2013-01-01
Combining the Capacitively Coupled Contactless Conductivity Detection (C4D) technique and the principle of cross correlation flow measurement, a new method for flow rate measurement in millimeter-scale pipes was proposed. The research work included two parts. First, a new five-electrode C4D sensor was developed. Second, with two conductivity signals obtained by the developed sensor, the flow rate measurement was implemented by using the principle of cross correlation flow measurement. The experimental results showed that the proposed flow rate measurement method was effective, the developed five-electrode C4D sensor was successful, and the measurement accuracy was satisfactory. In five millimeter-scale pipes with different inner diameters of 0.5, 0.8, 1.8, 3.0 and 3.9 mm respectively, the maximum relative difference of the flow rate measurement between the reference flow rate and the measured flow rate was less than 5%. PMID:23353139
Computational Methods for Analyzing Fluid Flow Dynamics from Digital Imagery
Luttman, A.
2012-03-30
The main goal (long term) of this work is to perform computational dynamics analysis and quantify uncertainty from vector fields computed directly from measured data. Global analysis based on observed spatiotemporal evolution is performed by objective function based on expected physics and informed scientific priors, variational optimization to compute vector fields from measured data, and transport analysis proceeding with observations and priors. A mathematical formulation for computing flow fields is set up for computing the minimizer for the problem. An application to oceanic flow based on sea surface temperature is presented.
Simple Radiowave-Based Method For Measuring Peripheral Blood Flow Project
NASA Technical Reports Server (NTRS)
Oliva-Buisson, Yvette J.
2014-01-01
Project objective is to design small radio frequency based flow probes for the measurement of blood flow velocity in peripheral arteries such as the femoral artery and middle cerebral artery. The result will be the technological capability to measure peripheral blood flow rates and flow changes during various environmental stressors such as microgravity without contact to the individual being monitored. This technology may also lead to an easier method of detecting venous gas emboli during extravehicular activities.
NASA Astrophysics Data System (ADS)
Blais-Stevens, A.; Behnia, P.
2016-02-01
This research activity aimed at reducing risk to infrastructure, such as a proposed pipeline route roughly parallel to the Yukon Alaska Highway Corridor (YAHC), by filling geoscience knowledge gaps in geohazards. Hence, the Geological Survey of Canada compiled an inventory of landslides including debris flow deposits, which were subsequently used to validate two different debris flow susceptibility models. A qualitative heuristic debris flow susceptibility model was produced for the northern region of the YAHC, from Kluane Lake to the Alaska border, by integrating data layers with assigned weights and class ratings. These were slope angle, slope aspect, surficial geology, plan curvature, and proximity to drainage system. Validation of the model was carried out by calculating a success rate curve which revealed a good correlation with the susceptibility model and the debris flow deposit inventory compiled from air photos, high-resolution satellite imagery, and field verification. In addition, the quantitative Flow-R method was tested in order to define the potential source and debris flow susceptibility for the southern region of Kluane Lake, an area where documented debris flow events have blocked the highway in the past (e.g. 1988). Trial and error calculations were required for this method because there was not detailed information on the debris flows for the YAHC to allow us to define threshold values for some parameters when calculating source areas, spreading, and runout distance. Nevertheless, correlation with known documented events helped define these parameters and produce a map that captures most of the known events and displays debris flow susceptibility in other, usually smaller, steep channels that had not been previously documented.
NASA Astrophysics Data System (ADS)
Blais-Stevens, A.; Behnia, P.
2015-05-01
This research activity aimed at reducing risk to infrastructure, such as a proposed pipeline route roughly parallel to the Yukon Alaska Highway Corridor (YAHC) by filling geoscience knowledge gaps in geohazards. Hence, the Geological Survey of Canada compiled an inventory of landslides including debris flow deposits, which were subsequently used to validate two different debris flow susceptibility models. A qualitative heuristic debris flow susceptibility model was produced for the northern region of the YAHC, from Kluane Lake to the Alaska border, by integrating data layers with assigned weights and class ratings. These were slope angle, slope aspect (derived from a 5 m × 5 m DEM), surficial geology, permafrost distribution, and proximity to drainage system. Validation of the model was carried out by calculating a success rate curve which revealed a good correlation with the susceptibility model and the debris flow deposit inventory compiled from air photos, high resolution satellite imagery, and field verification. In addition, the quantitative Flow-R method was tested in order to define the potential source and debris flow susceptibility for the southern region of Kluane Lake, an area where documented debris flow events have blocked the highway in the past (e.g., 1988). Trial and error calculations were required for this method because there was not detailed information on the debris flows for the YAHC to allow us to define threshold values for some parameters when calculating source areas, spreading, and runout distance. Nevertheless, correlation with known documented events helped define these parameters and produce a map that captures most of the known events and displays debris flow susceptibility in other, usually smaller, steep channels that had not been previously documented.
Study of design and analysis methods for transonic flow
NASA Technical Reports Server (NTRS)
Murman, E. M.
1977-01-01
An airfoil design program and a boundary layer analysis were developed. Boundary conditions were derived for ventilated transonic wind tunnels and performing transonic windtunnel wall calculations. A computational procedure for rotational transonic flow in engine inlet throats was formulated. Results and conclusions are summarized.
NASA Astrophysics Data System (ADS)
Liu, Haixing; Savić, Dragan; Kapelan, Zoran; Zhao, Ming; Yuan, Yixing; Zhao, Hongbin
2014-07-01
Flow entropy is a measure of uniformity of pipe flows in water distribution systems. By maximizing flow entropy one can identify reliable layouts or connectivity in networks. In order to overcome the disadvantage of the common definition of flow entropy that does not consider the impact of pipe diameter on reliability, an extended definition of flow entropy, termed as diameter-sensitive flow entropy, is proposed. This new methodology is then assessed by using other reliability methods, including Monte Carlo Simulation, a pipe failure probability model, and a surrogate measure (resilience index) integrated with water demand and pipe failure uncertainty. The reliability assessment is based on a sample of WDS designs derived from an optimization process for each of the two benchmark networks. Correlation analysis is used to evaluate quantitatively the relationship between entropy and reliability. To ensure reliability, a comparative analysis between the flow entropy and the new method is conducted. The results demonstrate that the diameter-sensitive flow entropy shows consistently much stronger correlation with the three reliability measures than simple flow entropy. Therefore, the new flow entropy method can be taken as a better surrogate measure for reliability and could be potentially integrated into the optimal design problem of WDSs. Sensitivity analysis results show that the velocity parameters used in the new flow entropy has no significant impact on the relationship between diameter-sensitive flow entropy and reliability.
NASA Technical Reports Server (NTRS)
Zang, Thomas A.; Mathelin, Lionel; Hussaini, M. Yousuff; Bataille, Francoise
2003-01-01
This paper describes a fully spectral, Polynomial Chaos method for the propagation of uncertainty in numerical simulations of compressible, turbulent flow, as well as a novel stochastic collocation algorithm for the same application. The stochastic collocation method is key to the efficient use of stochastic methods on problems with complex nonlinearities, such as those associated with the turbulence model equations in compressible flow and for CFD schemes requiring solution of a Riemann problem. Both methods are applied to compressible flow in a quasi-one-dimensional nozzle. The stochastic collocation method is roughly an order of magnitude faster than the fully Galerkin Polynomial Chaos method on the inviscid problem.
An empirical method for estimating travel times for wet volcanic mass flows
Pierson, Thomas C.
1998-01-01
Travel times for wet volcanic mass flows (debris avalanches and lahars) can be forecast as a function of distance from source when the approximate flow rate (peak discharge near the source) can be estimated beforehand. The near-source flow rate is primarily a function of initial flow volume, which should be possible to estimate to an order of magnitude on the basis of geologic, geomorphic, and hydrologic factors at a particular volcano. Least-squares best fits to plots of flow-front travel time as a function of distance from source provide predictive second-degree polynomial equations with high coefficients of determination for four broad size classes of flow based on near-source flow rate: extremely large flows (>1 000 000 m3/s), very large flows (10 000–1 000 000 m3/s), large flows (1000–10 000 m3/s), and moderate flows (100–1000 m3/s). A strong nonlinear correlation that exists between initial total flow volume and flow rate for "instantaneously" generated debris flows can be used to estimate near-source flow rates in advance. Differences in geomorphic controlling factors among different flows in the data sets have relatively little effect on the strong nonlinear correlations between travel time and distance from source. Differences in flow type may be important, especially for extremely large flows, but this could not be evaluated here. At a given distance away from a volcano, travel times can vary by approximately an order of magnitude depending on flow rate. The method can provide emergency-management officials a means for estimating time windows for evacuation of communities located in hazard zones downstream from potentially hazardous volcanoes.
Comparison of Peak-Flow Estimation Methods for Small Drainage Basins in Maine
Hodgkins, Glenn A.; Hebson, Charles; Lombard, Pamela J.; Mann, Alexander
2007-01-01
Understanding the accuracy of commonly used methods for estimating peak streamflows is important because the designs of bridges, culverts, and other river structures are based on these flows. Different methods for estimating peak streamflows were analyzed for small drainage basins in Maine. For the smallest basins, with drainage areas of 0.2 to 1.0 square mile, nine peak streamflows from actual rainfall events at four crest-stage gaging stations were modeled by the Rational Method and the Natural Resource Conservation Service TR-20 method and compared to observed peak flows. The Rational Method had a root mean square error (RMSE) of -69.7 to 230 percent (which means that approximately two thirds of the modeled flows were within -69.7 to 230 percent of the observed flows). The TR-20 method had an RMSE of -98.0 to 5,010 percent. Both the Rational Method and TR-20 underestimated the observed flows in most cases. For small basins, with drainage areas of 1.0 to 10 square miles, modeled peak flows were compared to observed statistical peak flows with return periods of 2, 50, and 100 years for 17 streams in Maine and adjoining parts of New Hampshire. Peak flows were modeled by the Rational Method, the Natural Resources Conservation Service TR-20 method, U.S. Geological Survey regression equations, and the Probabilistic Rational Method. The regression equations were the most accurate method of computing peak flows in Maine for streams with drainage areas of 1.0 to 10 square miles with an RMSE of -34.3 to 52.2 percent for 50-year peak flows. The Probabilistic Rational Method was the next most accurate method (-38.5 to 62.6 percent). The Rational Method (-56.1 to 128 percent) and particularly the TR-20 method (-76.4 to 323 percent) had much larger errors. Both the TR-20 and regression methods had similar numbers of underpredictions and overpredictions. The Rational Method overpredicted most peak flows and the Probabilistic Rational Method tended to overpredict peak flows
System and method for bidirectional flow and controlling fluid flow in a conduit
Ortiz, M.G.
1999-03-23
A system for measuring bidirectional flow, including backflow, of fluid in a conduit is disclosed. The system utilizes a structural mechanism to create a pressure differential in the conduit. Pressure sensors are positioned upstream from the mechanism, at the mechanism, and downstream from the mechanism. Data from the pressure sensors are transmitted to a microprocessor or computer, and pressure differential detected between the pressure sensors is then used to calculate the backflow. Control signals may then be generated by the microprocessor or computer to shut off valves located in the conduit, upon the occurrence of backflow, or to control flow, total material dispersed, etc. in the conduit. 3 figs.
System and method for bidirectional flow and controlling fluid flow in a conduit
Ortiz, Marcos German
1999-01-01
A system for measuring bidirectional flow, including backflow, of fluid in a conduit. The system utilizes a structural mechanism to create a pressure differential in the conduit. Pressure sensors are positioned upstream from the mechanism, at the mechanism, and downstream from the mechanism. Data from the pressure sensors are transmitted to a microprocessor or computer, and pressure differential detected between the pressure sensors is then used to calculate the backflow. Control signals may then be generated by the microprocessor or computer to shut off valves located in the conduit, upon the occurrence of backflow, or to control flow, total material dispersed, etc. in the conduit.
Comparing two methods of simulating mirco-scale viscous flows in a porous channel
NASA Astrophysics Data System (ADS)
Gao, Hui; Han, Jie; Jin, Yan; Wang, Lian-Ping
2007-11-01
Water flows in natural soil porous media are important to colloid-facilitated transport of contaminants and other phenomena with groundwater as the carrier. The 3D micro-scale flow is complicated due to the complex geometry. The transport and deposition of colloids in such flows are affected by several physical and chemical forces involved. In this talk, we first compare two methods of simulating viscous flows in both 2D and 3D channels filled with glass-bead particles. The first method is Physalis developed by Prosperetti's group, at Johns Hopkins, based on solving the Navier-Stokes equation using a combination of numerical solution and local analytical Stokes flow representation. The second method is a meso-scale approach by solving a lattice Boltzmann equation. Specific implementation issues will be discussed. The two methods yield almost identical flows. Preliminary simulation results as well as parallel experimental results on colloid deposition in the porous channel will also be presented.
Expiratory flow limitation definition, mechanisms, methods, and significance.
Tantucci, Claudio
2013-01-01
When expiratory flow is maximal during tidal breathing and cannot be increased unless operative lung volumes move towards total lung capacity, tidal expiratory flow limitation (EFL) is said to occur. EFL represents a severe mechanical constraint caused by different mechanisms and observed in different conditions, but it is more relevant in terms of prevalence and negative consequences in obstructive lung diseases and particularly in chronic obstructive pulmonary disease (COPD). Although in COPD patients EFL more commonly develops during exercise, in more advanced disorder it can be present at rest, before in supine position, and then in seated-sitting position. In any circumstances EFL predisposes to pulmonary dynamic hyperinflation and its unfavorable effects such as increased elastic work of breathing, inspiratory muscles dysfunction, and progressive neuroventilatory dissociation, leading to reduced exercise tolerance, marked breathlessness during effort, and severe chronic dyspnea.
Method of producing monolithic ceramic cross-flow filter
Larsen, D.A.; Bacchi, D.P.; Connors, T.F.; Collins, E.L. III
1998-02-10
Ceramic filter of various configuration have been used to filter particulates from hot gases exhausted from coal-fired systems. Prior ceramic cross-flow filters have been favored over other types, but those previously have been assemblies of parts somehow fastened together and consequently subject often to distortion or delamination on exposure hot gas in normal use. The present new monolithic, seamless, cross-flow ceramic filters, being of one-piece construction, are not prone to such failure. Further, these new products are made by a novel casting process which involves the key steps of demolding the ceramic filter green body so that none of the fragile inner walls of the filter is cracked or broken. 2 figs.
Method of producing monolithic ceramic cross-flow filter
Larsen, David A.; Bacchi, David P.; Connors, Timothy F.; Collins, III, Edwin L.
1998-01-01
Ceramic filter of various configuration have been used to filter particulates from hot gases exhausted from coal-fired systems. Prior ceramic cross-flow filters have been favored over other types, but those previously horn have been assemblies of parts somehow fastened together and consequently subject often to distortion or delamination on exposure hot gas in normal use. The present new monolithic, seamless, cross-flow ceramic filters, being of one-piece construction, are not prone to such failure. Further, these new products are made by novel casting process which involves the key steps of demolding the ceramic filter green body so that none of the fragile inner walls of the filter is cracked or broken.
Expiratory flow limitation definition, mechanisms, methods, and significance.
Tantucci, Claudio
2013-01-01
When expiratory flow is maximal during tidal breathing and cannot be increased unless operative lung volumes move towards total lung capacity, tidal expiratory flow limitation (EFL) is said to occur. EFL represents a severe mechanical constraint caused by different mechanisms and observed in different conditions, but it is more relevant in terms of prevalence and negative consequences in obstructive lung diseases and particularly in chronic obstructive pulmonary disease (COPD). Although in COPD patients EFL more commonly develops during exercise, in more advanced disorder it can be present at rest, before in supine position, and then in seated-sitting position. In any circumstances EFL predisposes to pulmonary dynamic hyperinflation and its unfavorable effects such as increased elastic work of breathing, inspiratory muscles dysfunction, and progressive neuroventilatory dissociation, leading to reduced exercise tolerance, marked breathlessness during effort, and severe chronic dyspnea. PMID:23606962
Methods Used in Game Development to Foster FLOW
NASA Technical Reports Server (NTRS)
Jeppsen, Isaac Ben
2010-01-01
Games designed for entertainment have a rich history of providing compelling experiences. From consoles to PCs, games have managed to present intuitive and effective interfaces for a wide range of game styles to successfully allow users to "walk-up-and-play". Once a user is hooked, successful games artfully present challenging experiences just within reach of a user's ability, weaving each task and achievement into a compelling and engaging experience. In this paper, engagement is discussed in terms of the psychological theory of Flow. I argue that engagement should be one of the primary goals when developing a serious game and I discuss the best practices and techniques that have emerged from traditional video game development which help foster the creation of engaging, high Flow experiences.
System and method measuring fluid flow in a conduit
Ortiz, Marcos German; Kidd, Terrel G.
1999-01-01
A system for measuring fluid mass flow in a conduit in which there exists a pressure differential in the fluid between at least two spaced-apart locations in the conduit. The system includes a first pressure transducer disposed in the side of the conduit at a first location for measuring pressure of fluid at that location, a second or more pressure transducers disposed in the side of the conduit at a second location, for making multiple measurements of pressure of fluid in the conduit at that location, and a computer for computing the average pressure of the multiple measurements at the second location and for computing flow rate of fluid in the conduit from the pressure measurement by the first pressure transducer and from the average pressure calculation of the multiple measurements.
System and method measuring fluid flow in a conduit
Ortiz, M.G.; Kidd, T.G.
1999-05-18
A system is described for measuring fluid mass flow in a conduit in which there exists a pressure differential in the fluid between at least two spaced-apart locations in the conduit. The system includes a first pressure transducer disposed in the side of the conduit at a first location for measuring pressure of fluid at that location, a second or more pressure transducers disposed in the side of the conduit at a second location, for making multiple measurements of pressure of fluid in the conduit at that location, and a computer for computing the average pressure of the multiple measurements at the second location and for computing flow rate of fluid in the conduit from the pressure measurement by the first pressure transducer and from the average pressure calculation of the multiple measurements. 3 figs.
Solution of Reactive Compressible Flows Using an Adaptive Wavelet Method
NASA Astrophysics Data System (ADS)
Zikoski, Zachary; Paolucci, Samuel; Powers, Joseph
2008-11-01
This work presents numerical simulations of reactive compressible flow, including detailed multicomponent transport, using an adaptive wavelet algorithm. The algorithm allows for dynamic grid adaptation which enhances our ability to fully resolve all physically relevant scales. The thermodynamic properties, equation of state, and multicomponent transport properties are provided by CHEMKIN and TRANSPORT libraries. Results for viscous detonation in a H2:O2:Ar mixture, and other problems in multiple dimensions, are included.
NASA Astrophysics Data System (ADS)
Hentschel, Werner
Laser light sheet flow visualization is applied in the automobile industry with a view to the reduction of air resistance. Using high power lasers a plane is cut out of the 3-D flow field and the course of flow in the plane is analyzed. In vehicle aerodynamics the flow phenomena are mainly visualized with smoke in the tail region of automobiles and in the wake, in planes parallel as well as perpendicular to the flow direction. For the investigation of flow phenomena in the combustion chamber of Otto and Diesel engines, the laser light sheet method is used on a series motor with optical access, the so-called flow motor. Typical results and requirements for future automated evaluation methods are discussed.
An Empirical Method for Fast Prediction of Rarefied Flow Field around a Vertical Plate
NASA Astrophysics Data System (ADS)
He, Tao; Wang, Jiang-Feng
2016-06-01
Numerical study is conducted to investigate the effects of free-stream Knudsen (Kn) number on rarefied flow field around a vertical plate employing an unstructured DSMC method, and an empirical method for fast prediction of flow-field structure at different Kn numbers in a given inflow velocity is proposed. First, the flow at a velocity 7500m/s is simulated using a perfect-gas model with free-stream Kn changing from 0.035 to 13.36. The flow-field characteristics in these cases with varying Kn numbers are analyzed and a linear-expansion phenomenon as a function of the square of Kn is discovered. An empirical method is proposed for fast flow-field prediction at different Kn based on the least-square-fitting method. Further, the effects of chemical reactions on flow field are investigated to verify the applicability of the empirical method in the real gas conditions. Three of the cases in perfect-gas flow are simulated again by introducing five-species air chemical module. The flow properties with and without chemical reactions are compared. In the end, the variation of chemical-reaction flow field as a function of Kn is analyzed and it is shown that the empirical method are also suitable when considering chemical reactions.
Kinetic theory based new upwind methods for inviscid compressible flows
NASA Technical Reports Server (NTRS)
Deshpande, S. M.
1986-01-01
Two new upwind methods called the Kinetic Numerical Method (KNM) and the Kinetic Flux Vector Splitting (KFVS) method for the solution of the Euler equations have been presented. Both of these methods can be regarded as some suitable moments of an upwind scheme for the solution of the Boltzmann equation provided the distribution function is Maxwellian. This moment-method strategy leads to a unification of the Riemann approach and the pseudo-particle approach used earlier in the development of upwind methods for the Euler equations. A very important aspect of the moment-method strategy is that the new upwind methods satisfy the entropy condition because of the Boltzmann H-Theorem and suggest a possible way of extending the Total Variation Diminishing (TVD) principle within the framework of the H-Theorem. The ability of these methods in obtaining accurate wiggle-free solution is demonstrated by applying them to two test problems.
Tracking and Measurement of the Motion of Blood Cells Using Optical Flow Methods
Guo, Dongmin; Van de Ven, Anne L.; Zhou, Xiaobo
2014-01-01
The investigation of microcirculation is a critical task in biomedical and physiological research. In order to monitor human’s condition and develop effective therapies of some diseases, the microcirculation information, such as flow velocity and vessel density, must be evaluated in a noninvasive manner. As one of the tasks of microcirculation investigation, automatic blood cell tracking presents an effective approach to estimate blood flow velocity. Currently, the most common method for blood cell tracking is based on spatiotemporal image analysis, which has lots of limitations, such as the diameter of microvesssels cannot be too larger than blood cells or tracers, cells or tracers should have fixed velocity, and it requires the image with high qualification. In this paper, we propose an optical flow method for automatic cell tracking. The key algorithm of the method is to align an image to its neighbors in a large image collection consisting of a variety of scenes. Considering the method cannot solve the problems in all cases of cell movement, another optical flow method, SIFT (Scale Invariant Feature Transform) flow, is also presented. The experimental results show that both methods can track the cells accurately. Optical flow is specially robust to the case where the velocity of cell is unstable, while SIFT flow works well when there are large displacement of cell between two adjacent frames. Our proposed methods outperform other methods when doing in vivo cell tracking, which can be used to estimate the blood flow directly and help to evaluate other parameters in microcirculation. PMID:24058034
Method and apparatus to measure vapor pressure in a flow system
Grossman, Mark W.; Biblarz, Oscar
1991-01-01
The present invention is directed to a method for determining, by a condensation method, the vapor pressure of a material with a known vapor pressure versus temperature characteristic, in a flow system particularly in a mercury isotope enrichment process.
Method of characteristics for three-dimensional axially symmetrical supersonic flows.
NASA Technical Reports Server (NTRS)
Sauer, R
1947-01-01
An approximation method for three-dimensional axially symmetrical supersonic flows is developed; it is based on the characteristics theory (represented partly graphically, partly analytically). Thereafter this method is applied to the construction of rotationally symmetrical nozzles. (author)
NASA Technical Reports Server (NTRS)
Mohn, L. W.
1975-01-01
The use of the Boeing TEA-230 Subsonic Flow Analysis method as a primary design tool in the development of cruise overwing nacelle configurations is presented. Surface pressure characteristics at 0.7 Mach number were determined by the TEA-230 method for a selected overwing flow-through nacelle configuration. Results of this analysis show excellent overall agreement with corresponding wind tunnel data. Effects of the presence of the nacelle on the wing pressure field were predicted accurately by the theoretical method. Evidence is provided that differences between theoretical and experimental pressure distributions in the present study would not result in significant discrepancies in the nacelle lines or nacelle drag estimates.
Method and system for measuring multiphase flow using multiple pressure differentials
Fincke, James R.
2001-01-01
An improved method and system for measuring a multiphase flow in a pressure flow meter. An extended throat venturi is used and pressure of the multiphase flow is measured at three or more positions in the venturi, which define two or more pressure differentials in the flow conduit. The differential pressures are then used to calculate the mass flow of the gas phase, the total mass flow, and the liquid phase. The method for determining the mass flow of the high void fraction fluid flow and the gas flow includes certain steps. The first step is calculating a gas density for the gas flow. The next two steps are finding a normalized gas mass flow rate through the venturi and computing a gas mass flow rate. The following step is estimating the gas velocity in the venturi tube throat. The next step is calculating the pressure drop experienced by the gas-phase due to work performed by the gas phase in accelerating the liquid phase between the upstream pressure measuring point and the pressure measuring point in the venturi throat. Another step is estimating the liquid velocity in the venturi throat using the calculated pressure drop experienced by the gas-phase due to work performed by the gas phase. Then the friction is computed between the liquid phase and a wall in the venturi tube. Finally, the total mass flow rate based on measured pressure in the venturi throat is calculated, and the mass flow rate of the liquid phase is calculated from the difference of the total mass flow rate and the gas mass flow rate.
An Efficient Inverse Aerodynamic Design Method For Subsonic Flows
NASA Technical Reports Server (NTRS)
Milholen, William E., II
2000-01-01
Computational Fluid Dynamics based design methods are maturing to the point that they are beginning to be used in the aircraft design process. Many design methods however have demonstrated deficiencies in the leading edge region of airfoil sections. The objective of the present research is to develop an efficient inverse design method which is valid in the leading edge region. The new design method is a streamline curvature method, and a new technique is presented for modeling the variation of the streamline curvature normal to the surface. The new design method allows the surface coordinates to move normal to the surface, and has been incorporated into the Constrained Direct Iterative Surface Curvature (CDISC) design method. The accuracy and efficiency of the design method is demonstrated using both two-dimensional and three-dimensional design cases.
Fictitious domain method for fully resolved reacting gas-solid flow simulation
NASA Astrophysics Data System (ADS)
Zhang, Longhui; Liu, Kai; You, Changfu
2015-10-01
Fully resolved simulation (FRS) for gas-solid multiphase flow considers solid objects as finite sized regions in flow fields and their behaviours are predicted by solving equations in both fluid and solid regions directly. Fixed mesh numerical methods, such as fictitious domain method, are preferred in solving FRS problems and have been widely researched. However, for reacting gas-solid flows no suitable fictitious domain numerical method has been developed. This work presents a new fictitious domain finite element method for FRS of reacting particulate flows. Low Mach number reacting flow governing equations are solved sequentially on a regular background mesh. Particles are immersed in the mesh and driven by their surface forces and torques integrated on immersed interfaces. Additional treatments on energy and surface reactions are developed. Several numerical test cases validated the method and a burning carbon particles array falling simulation proved the capability for solving moving reacting particle cluster problems.
Solution of transonic flows by an integro-differential equation method
NASA Technical Reports Server (NTRS)
Ogana, W.
1978-01-01
Solutions of steady transonic flow past a two-dimensional airfoil are obtained from a singular integro-differential equation which involves a tangential derivative of the perturbation velocity potential. Subcritical flows are solved by taking central differences everywhere. For supercritical flows with shocks, central differences are taken in subsonic flow regions and backward differences in supersonic flow regions. The method is applied to a nonlifting parabolic-arc airfoil and to a lifting NACA 0012 airfoil. Results compare favorably with those of finite-difference schemes.
Method and apparatus for chemically altering fluids in continuous flow
Heath, W.O.; Virden, J.W. Jr.; Richardson, R.L.; Bergsman, T.M.
1993-10-19
The present invention relates to a continuous flow fluid reactor for chemically altering fluids. The reactor operates on standard frequency (50 to 60 Hz) electricity. The fluid reactor contains particles that are energized by the electricity to form a corona throughout the volume of the reactor and subsequently a non-equilibrium plasma that interacts with the fluid. Particles may form a fixed bed or a fluid bed. Electricity may be provided through electrodes or through an inductive coil. Fluids include gases containing exhaust products and organic fuels requiring oxidation. 4 figures.
Method and apparatus for chemically altering fluids in continuous flow
Heath, William O.; Virden, Jr., Judson W.; Richardson, R. L.; Bergsman, Theresa M.
1993-01-01
The present invention relates to a continuous flow fluid reactor for chemically altering fluids. The reactor operates on standard frequency (50 to 60 Hz) electricity. The fluid reactor contains particles that are energized by the electricity to form a corona throughout the volume of the reactor and subsequently a non-equilibrium plasma that interacts with the fluid. Particles may form a fixed bed or a fluid bed. Electricity may be provided through electrodes or through an inductive coil. Fluids include gases containing exhaust products and organic fuels requiring oxidation.
Wilson, John Thomas
2000-01-01
A mathematical technique of estimating low-flow frequencies from base-flow measurements was evaluated by using data for streams in Indiana. Low-flow frequencies at low- flow partial-record stations were estimated by relating base-flow measurements to concurrent daily flows at nearby streamflow-gaging stations (index stations) for which low-flowfrequency curves had been developed. A network of long-term streamflow-gaging stations in Indiana provided a sample of sites with observed low-flow frequencies. Observed values of 7-day, 10-year low flow and 7-day, 2-year low flow were compared to predicted values to evaluate the accuracy of the method. Five test cases were used to evaluate the method under a variety of conditions in which the location of the index station and its drainage area varied relative to the partial-record station. A total of 141 pairs of streamflow-gaging stations were used in the five test cases. Four of the test cases used one index station, the fifth test case used two index stations. The number of base-flow measurements was varied for each test case to see if the accuracy of the method was affected by the number of measurements used. The most accurate and least variable results were produced when two index stations on the same stream or tributaries of the partial-record station were used. All but one value of the predicted 7-day, 10-year low flow were within 15 percent of the values observed for the long-term continuous record, and all of the predicted values of the 7-day, 2-year lowflow were within 15 percent of the observed values. This apparent accuracy, to some extent, may be a result of the small sample set of 15. Of the four test cases that used one index station, the most accurate and least variable results were produced in the test case where the index station and partial-record station were on the same stream or on streams tributary to each other and where the index station had a larger drainage area than the partial-record station. In
High speed flow cytometer droplet formation system and method
Van den Engh, Ger
2000-01-01
A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.
Visualization of transitional pipe flow using the photochromic tracer method
NASA Astrophysics Data System (ADS)
Zalzal, P.; Ojha, M.; Ethier, C. R.; Cobbold, R. S. C.; Johnston, K. W.
1994-06-01
Two noninvasive measurement techniques are described for investigating transitional flow in a rigid straight tube. Photochromic trace recordings, representing fluid displacement profiles, were made within the core and at the leading and trailing edges of turbulent slugs generated by a disturbance at the tube inlet. Consistent photochromic trace recordings were made possible through the discovery that Doppler ultrasound can be used to detect the passage of turbulent slugs, thereby allowing precise timing of the photochromic traces. Upstream and downstream Doppler probes enabled the leading and trailing edge velocities of slugs to be measured and also enabled the laminar/turbulent transition regions to be investigated in detail. Trailing edge interface velocities were in excellent agreement with existing data, while leading edge velocities agreed less well. Turbulence generation at the trailing edge appeared to be associated with a localized vortical structure which originated in the near wall region and then grew toward the tube centerline. The generation of this vortex is postulated to be induced by a shear layer instability. Mean velocity profiles obtained from within the core of turbulent slugs were similar to those obtained for fully developed turbulent flow.
[Application of three heat pulse technique-based methods to determine the stem sap flow].
Wang, Sheng; Fan, Jun
2015-08-01
It is of critical importance to acquire tree transpiration characters through sap flow methodology to understand tree water physiology, forest ecology and ecosystem water exchange. Tri-probe heat pulse sensors, which are widely utilized in soil thermal parameters and soil evaporation measurement, were applied to implement Salix matsudana sap flow density (Vs) measurements via heat-ratio method (HRM), T-Max method (T-Max) and single-probe heat pulse probe (SHPP) method, and comparative analysis was conducted with additional Grainer's thermal diffusion probes (TDP) measured results. The results showed that, it took about five weeks to reach a stable measurement stage after TPHP installation, Vs measured with three methods in the early stage after installation was 135%-220% higher than Vs in the stable measurement stage, and Vs estimated via HRM, T-Max and SHPP methods were significantly linearly correlated with Vs estimated via TDP method, with R2 of 0.93, 0.73 and 0.91, respectively, and R2 for Vs measured by SHPP and HRM reached 0.94. HRM had relatively higher precision in measuring low rates and reverse sap flow. SHPP method seemed to be very promising to measure sap flow for configuration simplicity and high measuring accuracy, whereas it couldn' t distinguish directions of flow. T-Max method had relatively higher error in sap flow measurement, and it couldn' t measure sap flow below 5 cm3 · cm(-2) · h(-1), thus this method could not be used alone, however it could measure thermal diffusivity for calculating sap flow when other methods were imposed. It was recommended to choose a proper method or a combination of several methods to measure stem sap flow, based on specific research purpose.
[Application of three heat pulse technique-based methods to determine the stem sap flow].
Wang, Sheng; Fan, Jun
2015-08-01
It is of critical importance to acquire tree transpiration characters through sap flow methodology to understand tree water physiology, forest ecology and ecosystem water exchange. Tri-probe heat pulse sensors, which are widely utilized in soil thermal parameters and soil evaporation measurement, were applied to implement Salix matsudana sap flow density (Vs) measurements via heat-ratio method (HRM), T-Max method (T-Max) and single-probe heat pulse probe (SHPP) method, and comparative analysis was conducted with additional Grainer's thermal diffusion probes (TDP) measured results. The results showed that, it took about five weeks to reach a stable measurement stage after TPHP installation, Vs measured with three methods in the early stage after installation was 135%-220% higher than Vs in the stable measurement stage, and Vs estimated via HRM, T-Max and SHPP methods were significantly linearly correlated with Vs estimated via TDP method, with R2 of 0.93, 0.73 and 0.91, respectively, and R2 for Vs measured by SHPP and HRM reached 0.94. HRM had relatively higher precision in measuring low rates and reverse sap flow. SHPP method seemed to be very promising to measure sap flow for configuration simplicity and high measuring accuracy, whereas it couldn' t distinguish directions of flow. T-Max method had relatively higher error in sap flow measurement, and it couldn' t measure sap flow below 5 cm3 · cm(-2) · h(-1), thus this method could not be used alone, however it could measure thermal diffusivity for calculating sap flow when other methods were imposed. It was recommended to choose a proper method or a combination of several methods to measure stem sap flow, based on specific research purpose. PMID:26685585
Shaw, C G; Plewes, D B
1986-08-01
The pulsed-injection method for measuring the velocity of blood flow in intraarterial digital subtraction angiography is described. With this technique, contrast material is injected at a pulsing frequency as high as 15 Hz, so that two or more boluses can be imaged simultaneously. The velocity of flow is determined by measuring the spacing between the boluses and multiplying it by the pulsing frequency. Results of tests with phantoms correlate well with flow measurements obtained with a graduated cylinder for velocities ranging from 8 to 60 cm/sec. The potential of the method for time-dependent velocity measurement has been demonstrated with simulated pulsatile flows. PMID:3523598
Methods for Computationally Efficient Structured CFD Simulations of Complex Turbomachinery Flows
NASA Technical Reports Server (NTRS)
Herrick, Gregory P.; Chen, Jen-Ping
2012-01-01
This research presents more efficient computational methods by which to perform multi-block structured Computational Fluid Dynamics (CFD) simulations of turbomachinery, thus facilitating higher-fidelity solutions of complicated geometries and their associated flows. This computational framework offers flexibility in allocating resources to balance process count and wall-clock computation time, while facilitating research interests of simulating axial compressor stall inception with more complete gridding of the flow passages and rotor tip clearance regions than is typically practiced with structured codes. The paradigm presented herein facilitates CFD simulation of previously impractical geometries and flows. These methods are validated and demonstrate improved computational efficiency when applied to complicated geometries and flows.
Newton-Krylov-Schwarz methods in unstructured grid Euler flow
Keyes, D.E.
1996-12-31
Newton-Krylov methods and Krylov-Schwarz (domain decomposition) methods have begun to become established in computational fluid dynamics (CFD) over the past decade. The former employ a Krylov method inside of Newton`s method in a Jacobian-free manner, through directional differencing. The latter employ an overlapping Schwarz domain decomposition to derive a preconditioner for the Krylov accelerator that relies primarily on local information, for data-parallel concurrency. They may be composed as Newton-Krylov-Schwarz (NKS) methods, which seem particularly well suited for solving nonlinear elliptic systems in high-latency, distributed-memory environments. We give a brief description of this family of algorithms, with an emphasis on domain decomposition iterative aspects. We then describe numerical simulations with Newton-Krylov-Schwarz methods on an aerodynamic application emphasizing comparisons with a standard defect-correction approach and subdomain preconditioner consistency.
Potential flow around two-dimensional airfoils using a singular integral method
NASA Technical Reports Server (NTRS)
Nguyen, Yves; Wilson, Dennis
1987-01-01
The problem of potential flow around two-dimensional airfoils is solved by using a new singular integral method. The potential flow equations for incompressible potential flow are written in a singular integral equation. The equation is solved at N collocation points on the airfoil surface. A unique feature of this method is that the airfoil geometry is specified as an independent variable in the exact integral equation. Compared to other numerical methods, the present calculation procedure is much simpler and gives remarkable accuracy for many body shapes. An advantage of the present method is that it allows the inverse design calculation and the results are extremely accurate.
On the peculiarities of LDA method in two-phase flows with high concentrations of particles
NASA Astrophysics Data System (ADS)
Poplavski, S. V.; Boiko, V. M.; Nesterov, A. U.
2016-10-01
Popular applications of laser Doppler anemometry (LDA) in gas dynamics are reviewed. It is shown that the most popular method cannot be used in supersonic flows and two-phase flows with high concentrations of particles. A new approach to implementation of the known LDA method based on direct spectral analysis, which offers better prospects for such problems, is presented. It is demonstrated that the method is suitable for gas-liquid jets. Owing to the progress in laser engineering, digital recording of spectra, and computer processing of data, the method is implemented at a higher technical level and provides new prospects of diagnostics of high-velocity dense two-phase flows.
NASA Technical Reports Server (NTRS)
Strong, Stuart L.; Meade, Andrew J., Jr.
1992-01-01
Preliminary results are presented of a finite element/finite difference method (semidiscrete Galerkin method) used to calculate compressible boundary layer flow about airfoils, in which the group finite element scheme is applied to the Dorodnitsyn formulation of the boundary layer equations. The semidiscrete Galerkin (SDG) method promises to be fast, accurate and computationally efficient. The SDG method can also be applied to any smoothly connected airfoil shape without modification and possesses the potential capability of calculating boundary layer solutions beyond flow separation. Results are presented for low speed laminar flow past a circular cylinder and past a NACA 0012 airfoil at zero angle of attack at a Mach number of 0.5. Also shown are results for compressible flow past a flat plate for a Mach number range of 0 to 10 and results for incompressible turbulent flow past a flat plate. All numerical solutions assume an attached boundary layer.
Kingsmore, S.F.; Crockard, A.D.; Fay, A.C.; McNeill, T.A.; Roberts, S.D.; Thompson, J.M.
1988-01-01
Several flow cytometric methods for the measurement of circulating immune complexes (CIC) have recently become available. We report a Raji cell flow cytometric assay (FCMA) that uses aggregated human globulin (AHG) as primary calibrator. Technical advantages of the Raji cell flow cytometric assay are discussed, and its clinical usefulness is evaluated in a method comparison study with the widely used Raji cell immunoradiometric assay. FCMA is more precise and has greater analytic sensitivity for AHG. Diagnostic sensitivity by the flow cytometric method is superior in systemic lupus erythematosus (SLE), rheumatoid arthritis, and vasculitis patients: however, diagnostic specificity is similar for both assays, but the reference interval of FCMA is narrower. Significant correlations were found between CIC levels obtained with both methods in SLE, rheumatoid arthritis, and vasculitis patients and in longitudinal studies of two patients with cerebral SLE. The Raji cell FCMA is recommended for measurement of CIC levels to clinical laboratories with access to a flow cytometer.
Abdi, Reza; Yasi, Mehdi
2015-01-01
The assessment of environmental flows in rivers is of vital importance for preserving riverine ecosystem processes. This paper addresses the evaluation of environmental flow requirements in three reaches along a typical perennial river (the Zab transboundary river, in north-west Iran), using different hydraulic, hydrological and ecological methods. The main objective of this study came from the construction of three dams and inter-basin transfer of water from the Zab River to the Urmia Lake. Eight hydrological methods (i.e. Tennant, Tessman, flow duration curve analysis, range of variability approach, Smakhtin, flow duration curve shifting, desktop reserve and 7Q2&10 (7-day low flow with a 2- and 10-year return period)); two hydraulic methods (slope value and maximum curvature); and two habitat simulation methods (hydraulic-ecologic, and Q Equation based on water quality indices) were used. Ecological needs of the riverine key species (mainly Barbus capito fish), river geometries, natural flow regime and the environmental status of river management were the main indices for determining the minimum flow requirements. The results indicate that the order of 35%, 17% and 18% of the mean annual flow are to be maintained for the upper, middle and downstream river reaches, respectively. The allocated monthly flow rates in the three Dams steering program are not sufficient to preserve the Zab River life.
Numerical method for computing flow through partially saturated porous media
Eaton, R.R.
1983-01-01
This paper discusses the development of the finite element computer code SAGUARO which calculates the two-dimensional flow of mass and energy through porous media. The media may be saturated or partially saturated. SAGUARO solves the parabolic time-dependent mass transport equation which accounts for the presence of partially saturated zones through the use of highly non-linear material characteristic curves. The energy equation accounts for the possibility of partially-saturated regions by adjusting the thermal capacitances and thermal conductivities according to the volume fraction of water present in the local pores. The code capabilities are demonstrated through the presentation of a sample problem involving the one-dimensional calculation of simultaneous energy transfer and water infiltration into partially saturated hard rock.
Numerical method for computing flow through partially saturated porous media
NASA Astrophysics Data System (ADS)
Eaton, R. R.
This paper discusses the development of the finite element computer code SAGUARO which calculates the two-dimensional flow of mass and energy through porous media. The media may be saturated or partially saturated. SAGUARO solves the parabolic time-dependent mass transport equation which accounts for the presence of partially saturated zones through the use of highly non-linear material characteristic curves. The energy equation accounts for the possibility of partially-saturated regions by adjusting the thermal capacitances and thermal conductivities according to the volume fraction of water present in the local pores. The code capabilities are demonstrated through the presentation of a sample problem involving the one dimensional calculation of simultaneous energy transfer and water infiltration into partially saturated hard rock.
A Survey of Flow Cytometry Data Analysis Methods
Bashashati, Ali; Brinkman, Ryan R.
2009-01-01
Flow cytometry (FCM) is widely used in health research and in treatment for a variety of tasks, such as in the diagnosis and monitoring of leukemia and lymphoma patients, providing the counts of helper-T lymphocytes needed to monitor the course and treatment of HIV infection, the evaluation of peripheral blood hematopoietic stem cell grafts, and many other diseases. In practice, FCM data analysis is performed manually, a process that requires an inordinate amount of time and is error-prone, nonreproducible, nonstandardized, and not open for re-evaluation, making it the most limiting aspect of this technology. This paper reviews state-of-the-art FCM data analysis approaches using a framework introduced to report each of the components in a data analysis pipeline. Current challenges and possible future directions in developing fully automated FCM data analysis tools are also outlined. PMID:20049163
Method and apparatus for acoustically monitoring the flow of suspended solid particulate matter
Roach, Paul D.; Raptis, Apostolos C.
1982-01-01
A method and apparatus for monitoring char flow in a coal gasifier system cludes flow monitor circuits which measure acoustic attenuation caused by the presence of char in a char line and provide a char flow/no flow indication and an indication of relative char density. The flow monitor circuits compute the ratio of signals in two frequency bands, a first frequency band representative of background noise, and a second higher frequency band in which background noise is attenuated by the presence of char. Since the second frequency band contains higher frequencies, the ratio can be used to provide a flow/no flow indication. The second band can also be selected so that attenuation is monotonically related to particle concentration, providing a quantitative measure of char concentration.
Laboratory Evaluation of Air Flow Measurement Methods for Residential HVAC Returns
Walker, Iain; Stratton, Chris
2015-07-01
This project improved the accuracy of air flow measurements used in commissioning California heating and air conditioning systems in Title 24 (Building and Appliance Efficiency Standards), thereby improving system performance and efficiency of California residences. The research team at Lawrence Berkeley National Laboratory addressed the issue that typical tools used by contractors in the field to test air flows may not be accurate enough to measure return flows used in Title 24 applications. The team developed guidance on performance of current diagnostics as well as a draft test method for use in future evaluations. The series of tests performed measured air flow using a range of techniques and devices. The measured air flows were compared to reference air flow measurements using inline air flow meters built into the test apparatus. The experimental results showed that some devices had reasonable results (typical errors of 5 percent or less) but others had much bigger errors (up to 25 percent).
A Phase-Field Method for Simulating Fluid-Structure Interactions in Multi-Phase Flow
NASA Astrophysics Data System (ADS)
Zheng, Xiaoning; Karniadakis, George
2015-11-01
We investigate two-phase flow instabilities by numerical simulations of fluid structure interactions in two-phase flow. The first case is a flexible pipe conveying two fluids, which exhibits self-sustained oscillations at high Reynolds number and tension related parameter. Well-defined two-phase flow patterns, i.e., slug flow and bubbly flow, are observed. The second case is external two-phase cross flow past a circular cylinder, which induces a Kelvin-Helmholtz instability due to density stratification. We solve the Navier-Stokes equation coupled with the Cahn-Hilliard equation and the structure equation in an arbitrary Lagrangian Eulerian (ALE) framework. For the fluid solver, a spectral/hp element method is employed for spatial discretization and backward differentiation for time discretization. For the structure solver, a Galerkin method is used in Lagrangian coordinates for spatial discretization and the Newmark- β scheme for time discretization.
Simulation of vortex-dominated aerodynamic flows by a point-vortex method
Jia, Z.
1988-01-01
A numerical study was made to simulate vortex-dominated aerodynamic flows by the point-vortex method. Attention was divided into three different aspects: a nascent vortex-shedding algorithm, numerical demonstration of the point-vortex method, and the calculation of some example of aerodynamic interesting flows, which include two major categories: unsteady flow about a flat plate at a fixed angle of attack with and without a leading edge flap, and the transient, vortical cross flow produced by a slender delta wing. Evolution of the vortex traces, streamlines, surface pressure, and forces are studied. Flow features based on data obtained by different point-vortex shedding rates and different integration time steps and schemes are found to be consistent with each other on length and time scales comparable to as well as considerably smaller than those of the global flow.
Computation of steady axisymmetric flow using a one-dimensional time-dependent method
NASA Technical Reports Server (NTRS)
Walitt, L.
1974-01-01
An iterative numerical method for computing steady, three dimensional, viscous, compressible flow fields, about aerodynamic bodies was studied. In order to develop the iterative method economically, the primary emphasis was directed towards supersonic, axisymmetric flow. However, the technique readily extends to three spatial dimensions. The viscous flow field about a cone-cylinder-flare body was calculated and compared to existing experimental data. Numerical predictions of the cone boundary layer and the flow field shock wave structure agreed with corresponding measurements. A separation was calculated at the cylinder-flare junction in six iterations; however, the size of the vortex did not correspond to the measured size. It was estimated that fifty iterations would be required to properly define the vortex. It was concluded that the iteration technique is of limited value for plane two dimensional and axisymmetrix flows, but of great value for three-dimensional flows.
Apparatus and method for constant flow oxidizing of organic materials
Surma, Jeffrey E.; Nelson, Norvell; Steward, G. Anthony; Bryan, Garry H.
1999-01-01
The invention is a method and apparatus using high cerium concentration in the anolyte of an electrochemical cell to oxidize organic materials. The method and apparatus further use an ultrasonic mixer to enhance the oxidation rate of the organic material in the electrochemical cell. A reaction vessel provides an advantage of independent reaction temperature control and electrochemical cell temperature control. A separate or independent reaction vessel may be used without an ultrasonic mixer to oxidize gaseous phase organic materials.
NASA Astrophysics Data System (ADS)
Larat, Adam; Sabat, Macole; Vié, Aymeric; Chalons, Christophe; Massot, Marc
2014-11-01
The simulation of particle-laden flows is of primary importance for several industrial applications, like sprays in aeronautical combustors or particles in fluidized beds. Our focus is on Moment methods that describes the disperse phase as a continuum. The accuracy and performance of such approaches highly depends on the number of controlled moments for correctly describing the physics of the flow, but also on the numerics that are used to solve the continuous system of equations at a discrete level. In the present work, we investigate the use of Discontinuous Galerkin methods to solve for the convective part of the moment equations. By deriving realizability conditions on the moment system that are associated to a convex space, a projection strategy is used to maintain the solution in the realizable space. This method is applied to the resolution of the pressure less gas dynamics and the Anisotropic Gaussian moment approach, the former solving for low Stokes number flows where no Particle Trajectory Crossing occurs, while the latter is solving for moderate Stokes number flows and can handle PTC through a pressure tensor in the convective term. The strategy is assessed on turbulent flows through comparisons with Lagrangian results.
Method and apparatus for cold gas reinjection in through-flow and reverse-flow wave rotors
NASA Technical Reports Server (NTRS)
Nalim, M. Razi (Inventor); Paxson, Daniel E. (Inventor)
1999-01-01
A method and apparatus for cold gas reinjection in through-flow and reverse-flow wave rotors having a plurality of channels formed around a periphery thereof. A first port injects a supply of cool air into the channels. A second port allows the supply of cool air to exit the channels and flow to a combustor. A third port injects a supply of hot gas from the combustor into the channels. A fourth port allows the supply of hot gas to exit the channels and flow to a turbine. A diverting port and a reinjection port are connected to the second and third ports, respectively. The diverting port diverts a portion of the cool air exiting through the second port as reinjection air. The diverting port is fluidly connected to the reinjection port which reinjects the reinjection air back into the channels. The reinjection air evacuates the channels of the hot gas resident therein and cools the channel walls, a pair of end walls of the rotor, ducts communicating with the rotor and subsequent downstream components. In a second embodiment, the second port receives all of the cool air exiting the channels and the diverting port diverts a portion of the cool air just prior to the cool air flowing to the combustor.
Contrasting discharge computation methods in riverine and tidal-affected flows in Mississippi
Turnipseed, D.P.
2004-01-01
Recent advancements in acoustic science have improved the measurement of real-time flow conditions in complex open-channel flow systems with dynamic channel geometry, velocity distribution and direction, and other gradually varying hydraulic characteristics. In the lower Pascagoula River Basin, a drainage area of about 9,500 square miles in Mississippi, riverine and tidal-affected river reaches exist that exhibit fairly steady flows during and after rainfall runoff events, and unsteady flows during low flow, tidal-affected events. Fairly steady flows can be computed usually within 5 percent by using methods developed by the USGS. Accurate measurement and computation of varied, non-uniform open-channel hydraulic streamflow conditions have historically been difficult or impossible. Acoustic and conventional methodologies to measure velocity in an open-channel riverine and tidal-affected reach have been combined to compute continuous discharge during varied, nonuniform flows by using the relations of stage and area in concert with average velocity and index velocity. Due to the unique flow characteristics on the lower Pascagoula River in Mississippi, an independent means of computing high flows based on conventional methods of a log regression of stage and discharge for a range of stages was also used. The two methods were contrasted and had good correlation. Copyright ASCE 2004.
Eash, David A.; Barnes, Kimberlee K.
2012-01-01
-least-squares equations developed for estimating the harmonic-mean-flow statistic for each of the three regions range from 66.4 to 80.4 percent. The regression equations are applicable only to stream sites in Iowa with low flows not significantly affected by regulation, diversion, or urbanization and with basin characteristics within the range of those used to develop the equations. If the equations are used at ungaged sites on regulated streams, or on streams affected by water-supply and agricultural withdrawals, then the estimates will need to be adjusted by the amount of regulation or withdrawal to estimate the actual flow conditions if that is of interest. Caution is advised when applying the equations for basins with characteristics near the applicable limits of the equations and for basins located in karst topography. A test of two drainage-area ratio methods using 31 pairs of streamgages, for the annual 7-day mean low-flow statistic for a recurrence interval of 10 years, indicates a weighted drainage-area ratio method provides better estimates than regional regression equations for an ungaged site on a gaged stream in Iowa when the drainage-area ratio is between 0.5 and 1.4. These regression equations will be implemented within the U.S. Geological Survey StreamStats web-based geographic-information-system tool. StreamStats allows users to click on any ungaged site on a river and compute estimates of the seven selected statistics; in addition, 90-percent prediction intervals and the measured basin characteristics for the ungaged sites also are provided. StreamStats also allows users to click on any streamgage in Iowa and estimates computed for these seven selected statistics are provided for the streamgage.
NASA Astrophysics Data System (ADS)
Sikarwar, Nidhi
The noise produced by the low bypass ratio turbofan engines used to power fighter aircraft is a problem for communities near military bases and for personnel working in close proximity to the aircraft. For example, carrier deck personnel are subject to noise exposure that can result in Noise-Induced Hearing Loss which in-turn results in over a billion dollars of disability payments by the Veterans Administration. Several methods have been proposed to reduce the jet noise at the source. These methods include microjet injection of air or water downstream of the jet exit, chevrons, and corrugated nozzle inserts. The last method involves the insertion of corrugated seals into the diverging section of a military-style convergent-divergent jet nozzle (to replace the existing seals). This has been shown to reduce both the broadband shock-associated noise as well as the mixing noise in the peak noise radiation direction. However, the original inserts were designed to be effective for a take-off condition where the jet is over-expanded. The nozzle performance would be expected to degrade at other conditions, such as in cruise at altitude. A new method has been proposed to achieve the same effects as corrugated seals, but using fluidic inserts. This involves injection of air, at relatively low pressures and total mass flow rates, into the diverging section of the nozzle. These fluidic inserts" deflect the flow in the same way as the mechanical inserts. The fluidic inserts represent an active control method, since the injectors can be modified or turned off depending on the jet operating conditions. Noise reductions in the peak noise direction of 5 to 6 dB have been achieved and broadband shock-associated noise is effectively suppressed. There are multiple parameters to be considered in the design of the fluidic inserts. This includes the number and location of the injectors and the pressures and mass flow rates to be used. These could be optimized on an ad hoc basis with
NASA Astrophysics Data System (ADS)
Papanicolaou, N. C.; Aristotelous, A. C.
2015-10-01
In this work, we develop a High-Order Symmetric Interior Penalty (SIP) Discontinuous Galerkin (DG) Finite Element Method (FEM) to investigate convective flows in a rectangular cavity subject to both vertical and horizontal temperature gradients. The whole cavity is subject to gravity modulation (g-jitter), simulating a microgravity environment. The sensitivity of the bifurcation problem makes the use of a high-order accurate and efficient technique essential. Our method is validated by solving the plane-parallel flow problem and the results were found to be in good agreement with published results. The numerical method was designed to be easily extendable to even more complex flows.
NASA Astrophysics Data System (ADS)
Botezatu, N. P.
1980-04-01
Various flow measurement methods and their applications are reviewed in order to establish the best methods for rationalization and optimization of water consumption in a large industrial system. The methods discussed include volumetric and gravimetric techniques, Pitot and Venturi tubes, electromagnetic and ultrasonic flowmeters, counters, rotameters, and anemometers, as well as the use of classical and radioactive tracers. A comparative analysis of various methods and experimental results indicate that the method of radioactive tracers is the only universal method of measurement of fluid flows through pipes and channels for fluids of any physicochemical properties under any conditions.
A pressure correction method for the calculation of compressible chemical reacting flows
NASA Technical Reports Server (NTRS)
Chen, Z. J.; Chen, C. P.; Chen, Y. S.
1992-01-01
A recently developed noniterative method for the solution of the transient fluid flow equations at all speed is extended to handle chemical reacting flows. The species conservation equations are loosely coupled into the predictor/multicorrector sequence of the solution procedure. A split-operator method separates the chemical kinetics terms from the fluid-dynamical terms, as well as an implicit differencing method enhance the numerical stability. The method was applied for turbulent diffusion flame calculations and for the analyses of high pressure, axisymmetric turbulent hypersonic nozzle flows. The diffusion flame results were compared with a similar pressure method for fast chemistry integration scheme without operator-splitting. Simulations of the nozzle flow indicated that the nonideal intermolecular effects must be included in the analysis and design of high pressure hypersonic nozzle.
A scalable RBF-FD method for atmospheric flow
NASA Astrophysics Data System (ADS)
Tillenius, Martin; Larsson, Elisabeth; Lehto, Erik; Flyer, Natasha
2015-10-01
Radial basis function-generated finite difference (RBF-FD) methods have recently been proposed as very interesting for global scale geophysical simulations, and have been shown to outperform established pseudo-spectral and discontinuous Galerkin methods for shallow water test problems. In order to be competitive for very large scale simulations, the RBF-FD methods needs to be efficiently implemented for modern multicore based computer architectures. This is a challenging assignment, because the main computational operations are unstructured sparse matrix-vector multiplications, which in general scale poorly on multicore computers due to bandwidth limitations. However, with the task parallel implementation described here we achieve 60-100% of theoretical speedup within a shared memory node, and 80-100% of linear speedup across nodes. We present results for global shallow water benchmark problems with a 30 km resolution.
A higher order panel method for linearized supersonic flow
NASA Technical Reports Server (NTRS)
Ehlers, F. E.; Epton, M. A.; Johnson, F. T.; Magnus, A. E.; Rubbert, P. E.
1979-01-01
The basic integral equations of linearized supersonic theory for an advanced supersonic panel method are derived. Methods using only linear varying source strength over each panel or only quadratic doublet strength over each panel gave good agreement with analytic solutions over cones and zero thickness cambered wings. For three dimensional bodies and wings of general shape, combined source and doublet panels with interior boundary conditions to eliminate the internal perturbations lead to a stable method providing good agreement experiment. A panel system with all edges contiguous resulted from dividing the basic four point non-planar panel into eight triangular subpanels, and the doublet strength was made continuous at all edges by a quadratic distribution over each subpanel. Superinclined panels were developed and tested on s simple nacelle and on an airplane model having engine inlets, with excellent results.
Direct Numerical Simulation of Incompressible Pipe Flow Using a B-Spline Spectral Method
NASA Technical Reports Server (NTRS)
Loulou, Patrick; Moser, Robert D.; Mansour, Nagi N.; Cantwell, Brian J.
1997-01-01
A numerical method based on b-spline polynomials was developed to study incompressible flows in cylindrical geometries. A b-spline method has the advantages of possessing spectral accuracy and the flexibility of standard finite element methods. Using this method it was possible to ensure regularity of the solution near the origin, i.e. smoothness and boundedness. Because b-splines have compact support, it is also possible to remove b-splines near the center to alleviate the constraint placed on the time step by an overly fine grid. Using the natural periodicity in the azimuthal direction and approximating the streamwise direction as periodic, so-called time evolving flow, greatly reduced the cost and complexity of the computations. A direct numerical simulation of pipe flow was carried out using the method described above at a Reynolds number of 5600 based on diameter and bulk velocity. General knowledge of pipe flow and the availability of experimental measurements make pipe flow the ideal test case with which to validate the numerical method. Results indicated that high flatness levels of the radial component of velocity in the near wall region are physical; regions of high radial velocity were detected and appear to be related to high speed streaks in the boundary layer. Budgets of Reynolds stress transport equations showed close similarity with those of channel flow. However contrary to channel flow, the log layer of pipe flow is not homogeneous for the present Reynolds number. A topological method based on a classification of the invariants of the velocity gradient tensor was used. Plotting iso-surfaces of the discriminant of the invariants proved to be a good method for identifying vortical eddies in the flow field.
A method for measuring cooling air flow in base coolant passages of rotating turbine blades
NASA Technical Reports Server (NTRS)
Liebert, C. H.; Pollack, F. G.
1975-01-01
Method accurately determines actual coolant mass flow rate in cooling passages of rotating turbine blades. Total and static pressures are measured in blade base coolant passages. Mass flow rates are calculated from these measurements of pressure, measured temperature and known area.
Vortical Flow Prediction using an Adaptive Unstructured Grid Method. Chapter 11
NASA Technical Reports Server (NTRS)
Pirzadeh, Shahyar Z.
2009-01-01
A computational fluid dynamics (CFD) method has been employed to compute vortical flows around slender wing/body configurations. The emphasis of the paper is on the effectiveness of an adaptive grid procedure in "capturing" concentrated vortices generated at sharp edges or flow separation lines of lifting surfaces flying at high angles of attack. The method is based on a tetrahedral unstructured grid technology developed at the NASA Langley Research Center. Two steady-state, subsonic, inviscid and Navier-Stokes flow test cases are presented to demonstrate the applicability of the method for solving vortical flow problems. The first test case concerns vortex flow over a simple 65 delta wing with different values of leading-edge radius. Although the geometry is quite simple, it poses a challenging problem for computing vortices originating from blunt leading edges. The second case is that of a more complex fighter configuration. The superiority of the adapted solutions in capturing the vortex flow structure over the conventional unadapted results is demonstrated by comparisons with the wind-tunnel experimental data. The study shows that numerical prediction of vortical flows is highly sensitive to the local grid resolution and that the implementation of grid adaptation is essential when applying CFD methods to such complicated flow problems.
DEVELOPMENT OF LOW-DIFFUSION FLUX-SPLITTING METHODS FOR DENSE GAS-SOLID FLOWS
The development of a class of low-diffusion upwinding methods for computing dense gas-solid flows is presented in this work. An artificial compressibility/low-Mach preconditioning strategy is developed for a hyperbolic two-phase flow equation system consisting of separate solids ...
Taylor, M K; Ginsburg, J; Hickey, A; Gheyas, F
2000-06-24
The flow properties of typical tablet and capsule formulation excipients, active compounds, and representative formulation blends were tested with current and novel flow measurement techniques to identify a reliable bench test to quantify powder flow as a screening method in early tablet and capsule formulation development. Test methods employed were vibrating spatula, critical orifice, angle of repose, compressibility index, and avalanching analysis. Powder flow results from each method were compiled in a database, sorted, and compared. An empirical composite index was established and powder flow was ranked in accordance with formulator experience. Principal components analyses of the angle of repose, percent compressibility, and critical orifice of the powder materials were also performed. The first principal component accounted for 72.8% of data variability; scores associated with this principal component score can serve as an index of flowability. Data generated from vibrating spatula and avalanching methods were not reproducible and were inconsistent with formulator experience and cited vendor references for flow. Improvements of test instruments and further studies are necessary for better assessment of these approaches.
Emissivity corrected infrared method for imaging anomalous structural heat flows
Del Grande, Nancy K.; Durbin, Philip F.; Dolan, Kenneth W.; Perkins, Dwight E.
1995-01-01
A method for detecting flaws in structures using dual band infrared radiation. Heat is applied to the structure being evaluated. The structure is scanned for two different wavelengths and data obtained in the form of images. Images are used to remove clutter to form a corrected image. The existence and nature of a flaw is determined by investigating a variety of features.
NASA Technical Reports Server (NTRS)
Macrossan, M. N.
1995-01-01
The direct simulation Monte Carlo (DSMC) method is the established technique for the simulation of rarefied gas flows. In some flows of engineering interest, such as occur for aero-braking spacecraft in the upper atmosphere, DSMC can become prohibitively expensive in CPU time because some regions of the flow, particularly on the windward side of blunt bodies, become collision dominated. As an alternative to using a hybrid DSMC and continuum gas solver (Euler or Navier-Stokes solver) this work is aimed at making the particle simulation method efficient in the high density regions of the flow. A high density, infinite collision rate limit of DSMC, the Equilibrium Particle Simulation method (EPSM) was proposed some 15 years ago. EPSM is developed here for the flow of a gas consisting of many different species of molecules and is shown to be computationally efficient (compared to DSMC) for high collision rate flows. It thus offers great potential as part of a hybrid DSMC/EPSM code which could handle flows in the transition regime between rarefied gas flows and fully continuum flows. As a first step towards this goal a pure EPSM code is described. The next step of combining DSMC and EPSM is not attempted here but should be straightforward. EPSM and DSMC are applied to Taylor-Couette flow with Kn = 0.02 and 0.0133 and S(omega) = 3). Toroidal vortices develop for both methods but some differences are found, as might be expected for the given flow conditions. EPSM appears to be less sensitive to the sequence of random numbers used in the simulation than is DSMC and may also be more dissipative. The question of the origin and the magnitude of the dissipation in EPSM is addressed. It is suggested that this analysis is also relevant to DSMC when the usual accuracy requirements on the cell size and decoupling time step are relaxed in the interests of computational efficiency.
Rumenik, R.P.; Grubbs, J.W.
1996-01-01
Methods for estimating low-flow frequency characteristics at ungaged sites were developed for two areas in northern Florida. In the Yellow, Blackwater, Escambia, and Perdido River Basins study area (northwestern Florida), regional regression equations were developed for estimating the 7- and 30-day, 2- and 10-year low-flow characteristic (Q7,2, Q7,10, Q30,2, and Q30,10) by determining values of basin characteristics from digital Geographical Information System (GIS) coverages or hardcopy maps. A GIS, ARC-INFO, was used to quantify basin characteristics that were used in regression equations. Sources of digital data used in this analysis are elevation data, from a digital elevation model, stream length and location data from a digital hydrography coverage, and watershed boundaries digitized from topographic maps. The most accurate regression equations employed a basin characteristic that was based on a simple conceptual model of one- dimensional ground-water flow using Darcy's law. Slightly less accurate equations were obtained using drainage area as the only explanatory variable. The standard error of prediction for the Darcy and drainage area equations of Q7,2 was 65 and 74 percent, respectively; Q7,10, 58 and 62 percent, respectively; Q30,2, 51 and, 54 percent, respectively; and Q30,10, 44 and 51 percent, respectively. In the Santa Fe River Basin study area (northeastern Florida), a flow-routing method was used to estimate low-flow characteristics at ungaged sites from low stream- flow analyses based on records at gaged sites. The use of the flow-routing method is suggested for areas where regression analysis proves unsuccessful, where low-flow characteristics have been defined at a significant number of sites, and where information about the basin characteristics has been thoroughly researched. Low-flow frequency characteristics determined at 40 sites and measurements made during five synoptic runs in 1989-91 were used to develop a flow-routing method. Low-flow
Three-dimensional hypersonic rarefied flow calculations using direct simulation Monte Carlo method
NASA Technical Reports Server (NTRS)
Celenligil, M. Cevdet; Moss, James N.
1993-01-01
A summary of three-dimensional simulations on the hypersonic rarefied flows in an effort to understand the highly nonequilibrium flows about space vehicles entering the Earth's atmosphere for a realistic estimation of the aerothermal loads is presented. Calculations are performed using the direct simulation Monte Carlo method with a five-species reacting gas model, which accounts for rotational and vibrational internal energies. Results are obtained for the external flows about various bodies in the transitional flow regime. For the cases considered, convective heating, flowfield structure and overall aerodynamic coefficients are presented and comparisons are made with the available experimental data. The agreement between the calculated and measured results are very good.
Deng, Shijie; Wang, Peng; Liu, Shengnan; Zhao, Tianze; Xu, Shanzhi; Guo, Mingjiang; Yu, Xinglong
2016-01-01
A novel microfluidic flow rate detection method based on surface plasmon resonance (SPR) temperature imaging is proposed. The measurement is performed by space-resolved SPR imaging of the flow induced temperature variations. Theoretical simulations and analysis were performed to demonstrate a proof of concept using this approach. Experiments were implemented and results showed that water flow rates within a wide range of tens to hundreds of μL/min could be detected. The flow rate sensor is resistant to disturbances and can be easily integrated into microfluidic lab-on-chip systems. PMID:27347960
Method for estimating low-flow characteristics of ungaged streams in Indiana
Arihood, Leslie D.; Glatfelter, Dale R.
1991-01-01
Equations for estimating the 7-day, 2-year and 7oday, 10-year low flows at sites on ungaged streams are presented. Regression analysis was used to develop equations relating basin characteristics and low-flow characteristics at 82 gaging stations. Significant basin characteristics in the equations are contributing drainage area and flow-duration ratio, which is the 20-percent flow duration divided by the 90-percent flow duration. Flow-duration ratio has been regionalized for Indiana on a plate. Ratios for use in the equations are obtained from the plate. Drainage areas are determined from maps or are obtained from reports. The predictive capability of the method was determined by tests of the equations and of the flow-duration ratios on the plate. The accuracy of the equations alone was tested by estimating the low-flow characteristics at 82 gaging stations where flow-duration ratio is already known. In this case, the standard errors of estimate for 7-day, 2-year and 7-day, 10-year low flows are 19 and 28 percent. When flow-duration ratios for the 82 gaging stations are obtained from the map, the standard errors are 46 and 61 percent. However, when stations having drainage areas of less than 10 square miles are excluded from the test, the standard errors decrease to 38 and 49 percent. Standard errors increase when stations with small basins are included, probably because some of the flow-duration ratios obtained for these small basins are incorrect. Local geology and its effect on the ratio are not adequately reflected on the plate, which shows the regional variation in flow-duration ratio. In all the tests, no bias is apparent areally, with increasing drainage area or with increasing ratio. Guidelines and limitations should be considered when using the method. The method can be applied only at sites in the northern and central physiographic zones of the State. Low-flow characteristics cannot be estimated for regulated streams unless the amount of regulation is
Method for estimating low-flow characteristics of ungaged streams in Indiana
Arihood, L.D.; Glatfelter, D.R.
1986-01-01
Equations for estimating the 7-day, 2-yr and 7-day, 10-yr low flows at sites on ungaged streams are presented. Regression analysis was used to develop equations relating basin characteristics and low flow characteristics at 82 gaging stations. Significant basin characteristics in the equations are contributing drainage area and flow duration ratio, which is the 20% flow duration divided by the 90% flow duration. Flow duration ratio has been regionalized for Indiana on a plate. Ratios for use in the equations are obtained from this plate. Drainage areas are determined from maps or are obtained from reports. The predictive capability of the method was determined by tests of the equations and of the flow duration ratios on the plate. The accuracy of the equations alone was tested by estimating the low flow characteristics at 82 gaging stations where flow duration ratio is already known. In this case, the standard errors of estimate for 7-day, 2-yr and 7-day, 10-yr low flows are 19% and 28%. When flow duration ratios for the 82 gaging stations are obtained from the map, the standard errors are 46% and 61%. However, when stations with drainage areas < 10 sq mi are excluded from the test, the standard errors reduce to 38% and 49%. Standard errors increase when stations with small basins are included, probably because some of the flow duration ratios obtained for these small basins are incorrect. Local geology and its effect on the ratio are not adequately reflected on the plate, which shows the regional variation in flow duration ratio. In all the tests, no bias is apparent areally, with increasing drainage area or with increasing ratio. Guidelines and limitations should be considered when using the method. The method can be applied only at sites in the northern and the central physiographic zones of the state. Low flow characteristics cannot be estimated for regulated streams unless the amount of regulation is known so that the estimated low flow characteristic can be
Finite element methods for non-Newtonian flows
Gartling, D.K.
1986-01-01
The application of the finite element method to problems in non-Newtonian fluid mechanics is described. The formulation of the basic equations is presented for both inelastic and viscoelastic constitutive models. Solution algorithms for treating the material nonlinearities associated with inealstic fluids are described; typical solution procedures for the implicit stress-rate equations of viscoelastic fluids are also presented. Simple example analyses are included for both types of fluid models. 65 refs., 21 figs.
Emissivity corrected infrared method for imaging anomalous structural heat flows
Del Grande, N.K.; Durbin, P.F.; Dolan, K.W.; Perkins, D.E.
1995-08-22
A method for detecting flaws in structures using dual band infrared radiation is disclosed. Heat is applied to the structure being evaluated. The structure is scanned for two different wavelengths and data obtained in the form of images. Images are used to remove clutter to form a corrected image. The existence and nature of a flaw is determined by investigating a variety of features. 1 fig.
Sensitive and real-time method for evaluating corneal barrier considering tear flow.
Nakamura, Tadahiro; Teshima, Mugen; Kitahara, Takashi; Sasaki, Hitoshi; Uematsu, Masafumi; Kitaoka, Takashi; Nakashima, Mikiro; Nishida, Koyo; Nakamura, Junzo; Higuchi, Shun
2010-01-01
We developed a new electrophysiological method mimicking tear flow to evaluate the epithelial tight junction of rabbit cornea quantitatively. We investigated the effect of tear flow on the corneal damage induced by ophthalmic preservatives using this method. An Ussing chamber system with Ag/AgCl electrodes was used in the electrophysiological experiment. The excised rabbit cornea was mounted in the Ussing chamber and the precorneal solution in the chamber was perfused with a peristaltic pump at the rate of human tear flow. Corneal transepithelial electrical resistance (TEER) was monitored as corneal barrier ability. In the electrophysiological method mimicking tear flow, we observed stable TEER, which rapidly decreased with benzalkonium chloride (BAC), an eye drop preservative. Using this system, we first found that 0.004% BAC decreased corneal TEER reversibly. A high concentration of BAC showed strong irreversible damage to the tight junction. The influence of BAC on corneal TEER was not only concentration-dependent but also tear flow rate-dependent. The electrophysiological method mimicking tear flow was useful to evaluate the corneal barrier quantitatively. Using this method, we clarified that the tear flow was important to protect the corneal damage induced by preservatives.
Regalado, Carlos M; Ritter, Axel
2007-08-01
Calibration of the Granier thermal dissipation technique for measuring stem sap flow in trees requires determination of the temperature difference (DeltaT) between a heated and an unheated probe when sap flow is zero (DeltaT(max)). Classically, DeltaT(max) has been estimated from the maximum predawn DeltaT, assuming that sap flow is negligible at nighttime. However, because sap flow may continue during the night, the maximum predawn DeltaT value may underestimate the true DeltaT(max). No alternative method has yet been proposed to estimate DeltaT(max) when sap flow is non-zero at night. A sensitivity analysis is presented showing that errors in DeltaT(max) may amplify through sap flux density computations in Granier's approach, such that small amounts of undetected nighttime sap flow may lead to large diurnal sap flux density errors, hence the need for a correct estimate of DeltaT(max). By rearranging Granier's original formula, an optimization method to compute DeltaT(max) from simultaneous measurements of diurnal DeltaT and micrometeorological variables, without assuming that sap flow is negligible at night, is presented. Some illustrative examples are shown for sap flow measurements carried out on individuals of Erica arborea L., which has needle-like leaves, and Myrica faya Ait., a broadleaf species. We show that, although DeltaT(max) values obtained by the proposed method may be similar in some instances to the DeltaT(max) predicted at night, in general the values differ. The procedure presented has the potential of being applied not only to Granier's method, but to other heat-based sap flow systems that require a zero flow calibration, such as the Cermák et al. (1973) heat balance method and the T-max heat pulse system of Green et al. (2003).
Regalado, Carlos M; Ritter, Axel
2007-08-01
Calibration of the Granier thermal dissipation technique for measuring stem sap flow in trees requires determination of the temperature difference (DeltaT) between a heated and an unheated probe when sap flow is zero (DeltaT(max)). Classically, DeltaT(max) has been estimated from the maximum predawn DeltaT, assuming that sap flow is negligible at nighttime. However, because sap flow may continue during the night, the maximum predawn DeltaT value may underestimate the true DeltaT(max). No alternative method has yet been proposed to estimate DeltaT(max) when sap flow is non-zero at night. A sensitivity analysis is presented showing that errors in DeltaT(max) may amplify through sap flux density computations in Granier's approach, such that small amounts of undetected nighttime sap flow may lead to large diurnal sap flux density errors, hence the need for a correct estimate of DeltaT(max). By rearranging Granier's original formula, an optimization method to compute DeltaT(max) from simultaneous measurements of diurnal DeltaT and micrometeorological variables, without assuming that sap flow is negligible at night, is presented. Some illustrative examples are shown for sap flow measurements carried out on individuals of Erica arborea L., which has needle-like leaves, and Myrica faya Ait., a broadleaf species. We show that, although DeltaT(max) values obtained by the proposed method may be similar in some instances to the DeltaT(max) predicted at night, in general the values differ. The procedure presented has the potential of being applied not only to Granier's method, but to other heat-based sap flow systems that require a zero flow calibration, such as the Cermák et al. (1973) heat balance method and the T-max heat pulse system of Green et al. (2003). PMID:17472936
Gur, D.; Yonas, H.; Jackson, D.L.; Wolfson, S.K. Jr.; Rockette, H.; Good, W.F.; Cook, E.E.; Arena, V.C.; Willy, J.A.; Maitz, G.S.
1985-10-01
Simultaneous measurements of cerebral blood flow have been performed in baboons to assess the correlation between the acute and invasive nondiffusible microsphere technique and the noninvasive xenon-enhanced CT method. Blood flows in small tissue volumes (approximately 1 cm3) were directly compared. The results of these studies demonstrate a statistically significant association between the two methods (P less than .001). Similar correlations were obtained by both the Kendall tau (tau) and the Spearman (r) methods. The problems and limitations of such correlations are discussed.
A fast and accurate method to predict 2D and 3D aerodynamic boundary layer flows
NASA Astrophysics Data System (ADS)
Bijleveld, H. A.; Veldman, A. E. P.
2014-12-01
A quasi-simultaneous interaction method is applied to predict 2D and 3D aerodynamic flows. This method is suitable for offshore wind turbine design software as it is a very accurate and computationally reasonably cheap method. This study shows the results for a NACA 0012 airfoil. The two applied solvers converge to the experimental values when the grid is refined. We also show that in separation the eigenvalues remain positive thus avoiding the Goldstein singularity at separation. In 3D we show a flow over a dent in which separation occurs. A rotating flat plat is used to show the applicability of the method for rotating flows. The shown capabilities of the method indicate that the quasi-simultaneous interaction method is suitable for design methods for offshore wind turbine blades.
Measurement of the resistivity of porous materials with an alternating air-flow method.
Dragonetti, Raffaele; Ianniello, Carmine; Romano, Rosario A
2011-02-01
Air-flow resistivity is a main parameter governing the acoustic behavior of porous materials for sound absorption. The international standard ISO 9053 specifies two different methods to measure the air-flow resistivity, namely a steady-state air-flow method and an alternating air-flow method. The latter is realized by the measurement of the sound pressure at 2 Hz in a small rigid volume closed partially by the test sample. This cavity is excited with a known volume-velocity sound source implemented often with a motor-driven piston oscillating with prescribed area and displacement magnitude. Measurements at 2 Hz require special instrumentation and care. The authors suggest an alternating air-flow method based on the ratio of sound pressures measured at frequencies higher than 2 Hz inside two cavities coupled through a conventional loudspeaker. The basic method showed that the imaginary part of the sound pressure ratio is useful for the evaluation of the air-flow resistance. Criteria are discussed about the choice of a frequency range suitable to perform simplified calculations with respect to the basic method. These criteria depend on the sample thickness, its nonacoustic parameters, and the measurement apparatus as well. The proposed measurement method was tested successfully with various types of acoustic materials.
Evaluation of the vibratory feeder method for assessment of powder flow properties.
Bhattachar, Shobha N; Hedden, David B; Olsofsky, Angela M; Qu, Xianggui; Hsieh, Wen-Yaw; Canter, Kelly G
2004-01-28
The flow properties of pharmaceutical powders and blends used in solid oral dosage forms are an important consideration during dosage form development. The vibratory feeder method, a flow measurement technique that quantifies avalanche flow, has been adapted for measurement of the flow properties of common pharmaceutical powders used in solid oral dosage forms. The flow properties of 17 different powders were measured with the instrument, and the results are reported as a powder flow index (PFI). The PFI trends of the powders correlate well with flow properties reported in the literature. The flow properties of the powders were also measured with a commercially available avalanche instrument, the Aero-Flow, and the results were reported as the mean time to avalanche (MTA). Since the two instruments analyze the avalanche by different algorithms, the results were compared with nonparametric statistical evaluation of ranked data, and they were found to be in excellent agreement. A recommended procedure for measurement of powder flow with the vibratory feeder is presented.
Method for inhibiting silica precipitation and scaling in geothermal flow systems
Harrar, J.E.; Lorensen, L.E.; Locke, F.E.
1980-06-13
A method for inhibiting silica scaling and precipitation in geothermal flow systems by on-line injection of low concentrations of cationic nitrogen-containing compounds, particularly polymeric imines, polymeric amines, and quaternary ammonium compounds is described.
Method for inhibiting silica precipitation and scaling in geothermal flow systems
Harrar, Jackson E.; Lorensen, Lyman E.; Locke, Frank E.
1982-01-01
A method for inhibiting silica scaling and precipitation in geothermal flow systems by on-line injection of low concentrations of cationic nitrogen-containing compounds, particularly polymeric imines, polymeric amines, and quaternary ammonium compounds.
A method for calculating turbulent boundary layers and losses in the flow channels of turbomachines
NASA Technical Reports Server (NTRS)
Schumann, Lawrence F.
1987-01-01
An interactive inviscid core flow-boundary layer method is presented for the calculation of turbomachine channel flows. For this method, a one-dimensional inviscid core flow is assumed. The end-wall and blade surface boundary layers are calculated using an integral entrainment method. The boundary layers are assumed to be collateral and thus are two-dimensional. The boundary layer equations are written in a streamline coordinate system. The streamwise velocity profiles are approximated by power law profiles. Compressibility is accounted for in the streamwise direction but not in the normal direction. Equations are derived for the special cases of conical and two-dimensional rectangular diffusers. For these cases, the assumptions of a one-dimensional core flow and collateral boundary layers are valid. Results using the method are compared with experiment and good quantitative agreement is obtained.
Method For Enhanced Gas Monitoring In High Density Flow Streams
Von Drasek, William A.; Mulderink, Kenneth A.; Marin, Ovidiu
2005-09-13
A method for conducting laser absorption measurements in high temperature process streams having high levels of particulate matter is disclosed. An impinger is positioned substantially parallel to a laser beam propagation path and at upstream position relative to the laser beam. Beam shielding pipes shield the beam from the surrounding environment. Measurement is conducted only in the gap between the two shielding pipes where the beam propagates through the process gas. The impinger facilitates reduced particle presence in the measurement beam, resulting in improved SNR (signal-to-noise) and improved sensitivity and dynamic range of the measurement.
Solution of non-isoenergetic supersonic flows by method of characteristics, volume 3
NASA Technical Reports Server (NTRS)
Prozan, R. J.
1972-01-01
The calculation of supersonic flow fields by the method of characteristics. The theoretical approach to the solution of these flow fields and a computer program to implement the numerical solution of the flow equations are discussed. This versatile program has a flexible set of boundary conditions enabling the calculation of nozzles, plumes and many other complex flow fields. A complete derivation of the equations of motion for reacting gas systems is presented. An important consequence of this derivation is that, for the reaction assumptions which were made, the thermochemistry was shown to be uncoupled from the flow solution and as such could be solved separately. The methods of characteristics equations are shown to be formally the same for ideal, frozen, and equilibrium reacting gas mixtures.
Method and apparatus for measuring the mass flow rate of a fluid
Evans, Robert P.; Wilkins, S. Curtis; Goodrich, Lorenzo D.; Blotter, Jonathan D.
2002-01-01
A non invasive method and apparatus is provided to measure the mass flow rate of a multi-phase fluid. An accelerometer is attached to a pipe carrying a multi-phase fluid. Flow related measurements in pipes are sensitive to random velocity fluctuations whose magnitude is proportional to the mean mass flow rate. An analysis of the signal produced by the accelerometer shows a relationship between the mass flow of a fluid and the noise component of the signal of an accelerometer. The noise signal, as defined by the standard deviation of the accelerometer signal allows the method and apparatus of the present invention to non-intrusively measure the mass flow rate of a multi-phase fluid.
Spectral Element Method for the Simulation of Unsteady Compressible Flows
NASA Technical Reports Server (NTRS)
Diosady, Laslo Tibor; Murman, Scott M.
2013-01-01
This work uses a discontinuous-Galerkin spectral-element method (DGSEM) to solve the compressible Navier-Stokes equations [1{3]. The inviscid ux is computed using the approximate Riemann solver of Roe [4]. The viscous fluxes are computed using the second form of Bassi and Rebay (BR2) [5] in a manner consistent with the spectral-element approximation. The method of lines with the classical 4th-order explicit Runge-Kutta scheme is used for time integration. Results for polynomial orders up to p = 15 (16th order) are presented. The code is parallelized using the Message Passing Interface (MPI). The computations presented in this work are performed using the Sandy Bridge nodes of the NASA Pleiades supercomputer at NASA Ames Research Center. Each Sandy Bridge node consists of 2 eight-core Intel Xeon E5-2670 processors with a clock speed of 2.6Ghz and 2GB per core memory. On a Sandy Bridge node the Tau Benchmark [6] runs in a time of 7.6s.
Projection Method for Flows with Large Density Variations
NASA Technical Reports Server (NTRS)
Heinrich, Juan C.; Westra, Douglas G.
2007-01-01
Numerical models of solidification including a mushy zone are notoriously inefficient; most of them are based on formulations that require the coupled solution of the velocity components in the momentum equation greatly restricting the range of applicability of the models. There are only two models known to the authors that have used a projection or fractional step formulation, but none of these were used to model problems of any significant size. A third model was only applied to a partial mushy zone with no all-fluid region. Our initial attempts at modeling directional solidification in the presence of a developing mushy zone using a projection formulation encountered very serious difficulties once solidification starts. These difficulties were traced to the inability of the method to deal with large local density differences in the vicinity of the fluid-mush interface. As a result, a modified formulation of the projection method has been developed, that maintains the coupling between the body force and the pressure gradient and is presented in this work. The new formulation is shown to be robust and efficient, and can be applied to problems involving very large meshes. This is illustrated in this work through its application to simulations involving Pb-Sb and Pb-Sn alloys.
Koltun, G.F.; Kula, Stephanie P.
2013-01-01
This report presents the results of a study to develop methods for estimating selected low-flow statistics and for determining annual flow-duration statistics for Ohio streams. Regression techniques were used to develop equations for estimating 10-year recurrence-interval (10-percent annual-nonexceedance probability) low-flow yields, in cubic feet per second per square mile, with averaging periods of 1, 7, 30, and 90-day(s), and for estimating the yield corresponding to the long-term 80-percent duration flow. These equations, which estimate low-flow yields as a function of a streamflow-variability index, are based on previously published low-flow statistics for 79 long-term continuous-record streamgages with at least 10 years of data collected through water year 1997. When applied to the calibration dataset, average absolute percent errors for the regression equations ranged from 15.8 to 42.0 percent. The regression results have been incorporated into the U.S. Geological Survey (USGS) StreamStats application for Ohio (http://water.usgs.gov/osw/streamstats/ohio.html) in the form of a yield grid to facilitate estimation of the corresponding streamflow statistics in cubic feet per second. Logistic-regression equations also were developed and incorporated into the USGS StreamStats application for Ohio for selected low-flow statistics to help identify occurrences of zero-valued statistics. Quantiles of daily and 7-day mean streamflows were determined for annual and annual-seasonal (September–November) periods for each complete climatic year of streamflow-gaging station record for 110 selected streamflow-gaging stations with 20 or more years of record. The quantiles determined for each climatic year were the 99-, 98-, 95-, 90-, 80-, 75-, 70-, 60-, 50-, 40-, 30-, 25-, 20-, 10-, 5-, 2-, and 1-percent exceedance streamflows. Selected exceedance percentiles of the annual-exceedance percentiles were subsequently computed and tabulated to help facilitate consideration of the
Characteristics method with cubic-spline interpolation for open channel flow computation
NASA Astrophysics Data System (ADS)
Tsai, Tung-Lin; Chiang, Shih-Wei; Yang, Jinn-Chuang
2004-10-01
In the framework of the specified-time-interval scheme, the accuracy of the characteristic method is greatly related to the form of the interpolation. The linear interpolation was commonly used to couple the characteristics method (LI method) in open channel flow computation. The LI method is easy to implement, but it leads to an inevitable smoothing of the solution. The characteristics method with the Hermite cubic interpolation (HP method, originally developed by Holly and Preissmann, 1977) was then proposed to largely reduce the error induced by the LI method. In this paper, the cubic-spline interpolation on the space line or on the time line is employed to integrate with characteristics method (CS method) for unsteady flow computation in open channel. Two hypothetical examples, including gradually and rapidly varied flows, are used to examine the applicability of the CS method as compared with the LI method, the HP method, and the analytical solutions. The simulated results show that the CS method is comparable to the HP method and more accurate than the LI method. Without tackling the additional equations for spatial or temporal derivatives, the CS method is easier to implement and more efficient than the HP method.
Contactless flow measurement in liquid metal using electromagnetic time-of-flight method
NASA Astrophysics Data System (ADS)
Dubovikova, Nataliia; Resagk, Christian; Karcher, Christian; Kolesnikov, Yuri
2016-05-01
Measuring flow rates of liquid metal flows is of utmost importance in industrial applications such as metal casting, in order to ensure process efficiency and product quality. A non-contact method for flow rate control is described here. The method is known as time-of-flight Lorentz force velocimetry (LFV) and determines flow rate through measurement of Lorentz force that act on magnet systems that are placed close to the flow. In this method, a vortex generator is used to generate an eddy in the flow, with two magnet systems separated by a known distance placed downstream of the vortex generator. Each of the magnet systems has a force sensor attached to them which detects the passing of the eddy through its magnetic field as a significant perturbation in the force signal. The flow rate is estimated from the time span between the perturbations in the two force signals. In this paper, time-of-flight LFV technique is demonstrated experimentally for the case of liquid metal flow in a closed rectangular duct loop that is driven by an electromagnetic pump. A liquid metal alloy of gallium (Ga), indium (In) and tin (Sn)—GaInSn—is used as the working fluid. In contrast to prior works, for the first time, three-dimensional strain gauge force sensors were used for measuring Lorentz force to investigate the effect of flow disturbances in different directions for flow measurements by the time-of-flight LFV method. A prototype time-of-flight LFV flowmeter is developed, the operation of which in laboratory conditions is characterised by different experiments.
Solutions of turbulent backward-facing step flow with heat transfer using the finite volume method
Horstman, R.H.; Cochran, R.J.; Emergy, A.F.
1995-12-31
The heated turbulent flow over a backward-facing step is numerically solved using the commercial computational fluid dynamics program FLUENT. The methods used here consist of the default power-law upwinding scheme, default multigrid equation solution method and a standard k-{var_epsilon} turbulence model with wall functions. A total of four separate cases are reported. The four cases consist of combinations of partially and fully developed flow at the inlet with uniform or developed temperature profiles. Three mesh refinements are reported for each flow.
Simulation of Blood Flow at Vessel Bifurcation by Lattice Boltzmann Method
NASA Astrophysics Data System (ADS)
Kang, Xiu-Ying; Liu, Da-He; Zhou, Jing; Jin, Yong-Juan
2005-11-01
The application of the lattice Boltzmann method to the large vessel bifurcation blood flow is investigated in a wide range of Reynolds numbers. The velocity, shear stress and pressure distributions at the bifurcation are presented in detail. The flow separation zones revealed with increase of Reynolds number are located in the areas of the daughter branches distal to the outer corners of the bifurcation where some deposition of particular blood components might occur to form arteriosclerosis. The results also demonstrate that the lattice Boltzmann method is adaptive to simulating the flow in larger vessels under a high Reynolds number.
New method of asymmetric flow field measurement in hypersonic shock tunnel.
Yan, D P; He, A Z; Ni, X W
1991-03-01
In this paper a method of large aperture (?500 mm) high sensitivity moire deflectometry is used to obtain multidirectional deflectograms of the asymmetric flow field in hypersonic (M = 10.29) shock tunnel. At the same time, a 3-D reconstructive method of the asymmetric flow field is presented which is based on the integration of the moire deflective angle and the double-cubic many-knot interpolating splines; it is used to calculate the 3-D density distribution of the asymmetric flow field.
Application of shock capturing and characteristics methods to shuttle flow fields
NASA Technical Reports Server (NTRS)
Kutler, P.; Rakich, J. V.; Mateer, G. G.
1972-01-01
The calculation of real gas flows around the space shuttle vehicle can be accomplished by the method of characteristics (MOC) or shock capture technique (SCT). Results obtained with these methods for an early orbiter shape proposed by the North American Rockwell Corporation and also for the more recent 040A configuration suggested by NASA-MSC are presented. Results obtained with the SCT code demonstrate its three-dimensional, multiple shock capturing capability while results obtained with the MOC code demonstrate the calculation of equilibrium real gas flows and the determination of flow variables required for a heating analysis.
NASA Astrophysics Data System (ADS)
Sanmiguel-Rojas, Enrique; Ortega-Casanova, Joaquin; del Pino, Carlos; Fernandez-Feria, Ramon
2004-11-01
A method for generating a non-uniform cartesian grid for irregular two-dimensional (2D) geometries such that all the boundary points are regular mesh points is given. The resulting non-uniform grid is used to discretize the Navier-Stokes equations for 2D incompressible viscous flows using finite difference approximations. To that end, finite-difference approximations of the derivatives on a non-uniform mesh are given. We test the method with two different examples: the shallow water flow on a lake with irregular contour, and the pressure driven flow through an irregular array of circular cylinders.
A measurement method of the flow rate in a pipe using a microphone array
NASA Astrophysics Data System (ADS)
Kim, Yong-Beum; Kim, Yang-Hann
2002-09-01
A method of measuring the flow rate in a pipe is proposed. The method utilizes one-dimensional acoustic pressure signals that are generated by a loud speaker. A microphone array mounted flush with the inner pipe wall is used to measure the signals. A formula for the flow rate, which is a function of the change of wave number, is derived from a simple mathematical model of sound field in the pipe conveying a viscous fluid. The change of the wave number, which is one of the results caused by flow, is estimated from the recursive relation among the measured microphone array signals. Since measurement errors, due to extraneous measurement noise and mismatch of response characteristics between microphones, exist in the estimated flow rate, a method of compensating the errors is proposed. By using this measurement method, the flow rate can be obtained more accurately than that of our previous method. To verify applicability of the measurement method, numerical simulation and experiments are performed. The estimated flow rates are within 5% error bound. copyright 2002 Acoustical Society of America.
Frequency-domain Monte Carlo method for linear oscillatory gas flows
NASA Astrophysics Data System (ADS)
Ladiges, Daniel R.; Sader, John E.
2015-03-01
Gas flows generated by resonating nanoscale devices inherently occur in the non-continuum, low Mach number regime. Numerical simulations of such flows using the standard direct simulation Monte Carlo (DSMC) method are hindered by high statistical noise, which has motivated the development of several alternate Monte Carlo methods for low Mach number flows. Here, we present a frequency-domain low Mach number Monte Carlo method based on the Boltzmann-BGK equation, for the simulation of oscillatory gas flows. This circumvents the need for temporal simulations, as is currently required, and provides direct access to both amplitude and phase information using a pseudo-steady algorithm. The proposed method is validated for oscillatory Couette flow and the flow generated by an oscillating sphere. Good agreement is found with an existing time-domain method and accurate numerical solutions of the Boltzmann-BGK equation. Analysis of these simulations using a rigorous statistical approach shows that the frequency-domain method provides a significant improvement in computational speed.
Flow Applications of the Least Squares Finite Element Method
NASA Technical Reports Server (NTRS)
Jiang, Bo-Nan
1998-01-01
The main thrust of the effort has been towards the development, analysis and implementation of the least-squares finite element method (LSFEM) for fluid dynamics and electromagnetics applications. In the past year, there were four major accomplishments: 1) special treatments in computational fluid dynamics and computational electromagnetics, such as upwinding, numerical dissipation, staggered grid, non-equal order elements, operator splitting and preconditioning, edge elements, and vector potential are unnecessary; 2) the analysis of the LSFEM for most partial differential equations can be based on the bounded inverse theorem; 3) the finite difference and finite volume algorithms solve only two Maxwell equations and ignore the divergence equations; and 4) the first numerical simulation of three-dimensional Marangoni-Benard convection was performed using the LSFEM.
The Chimera Method of Simulation for Unsteady Three-Dimensional Viscous Flow
NASA Technical Reports Server (NTRS)
Meakin, Robert L.
1996-01-01
The Chimera overset grid method is reviewed and discussed in the context of a method of solution and analysis of unsteady three-dimensional viscous flows. The state of maturity of the various pieces of support software required to use the approach is discussed. A variety of recent applications of the method is presented. Current limitations of the approach are defined.
A Conceptual Framework for the Indirect Method of Reporting Net Cash Flow from Operating Activities
ERIC Educational Resources Information Center
Wang, Ting J.
2010-01-01
This paper describes the fundamental concept of the reconciliation behind the indirect method of the statement of cash flows. A conceptual framework is presented to demonstrate how accrual and cash-basis accounting methods relate to each other and to illustrate the concept of reconciling these two accounting methods. The conceptual framework…
Shaw, Emily C; Phinn, Stuart R; Tilbrook, Bronte; Steven, Andy
2014-01-01
Coral reef calcification is predicted to decline as a result of ocean acidification and other anthropogenic stressors. The majority of studies predicting declines based on in situ relationships between environmental parameters and net community calcification rate have been location-specific, preventing accurate predictions for coral reefs globally. In this study, net community calcification and production were measured on a coral reef flat at One Tree Island, Great Barrier Reef, using Lagrangian flow respirometry and slack water methods. Net community calcification, daytime net photosynthesis and nighttime respiration were higher under the flow respirometry method, likely due to increased water flow relative to the slack water method. The two methods also varied in the degrees to which they were influenced by potential measurement uncertainties. The difference in the results from these two commonly used methods implies that some of the location-specific differences in coral reef community metabolism may be due to differences in measurement methods.
Shaw, Emily C.; Phinn, Stuart R.; Tilbrook, Bronte; Steven, Andy
2014-01-01
Coral reef calcification is predicted to decline as a result of ocean acidification and other anthropogenic stressors. The majority of studies predicting declines based on in situ relationships between environmental parameters and net community calcification rate have been location-specific, preventing accurate predictions for coral reefs globally. In this study, net community calcification and production were measured on a coral reef flat at One Tree Island, Great Barrier Reef, using Lagrangian flow respirometry and slack water methods. Net community calcification, daytime net photosynthesis and nighttime respiration were higher under the flow respirometry method, likely due to increased water flow relative to the slack water method. The two methods also varied in the degrees to which they were influenced by potential measurement uncertainties. The difference in the results from these two commonly used methods implies that some of the location-specific differences in coral reef community metabolism may be due to differences in measurement methods. PMID:25426626
A comparison of several methods of solving nonlinear regression groundwater flow problems.
Cooley, R.L.
1985-01-01
Computational efficiency and computer memory requirements for four methods of minimizing functions were compared for four test nonlinear-regression steady state groundwater flow problems. The fastest methods were the Marquardt and quasi-linearization methods, which required almost identical computer times and numbers of iterations; the next fastest was the quasi-Newton method, and last was the Fletcher-Reeves method, which did not converge in 100 iterations for two of the problems.-from Author
Investigation of an innovative method for DC flow suppression of double-inlet pulse tube coolers
NASA Astrophysics Data System (ADS)
Hu, J. Y.; Luo, E. C.; Wu, Z. H.; Dai, W.; Zhu, S. L.
2007-05-01
The use of double-inlet mode in the pulse tube cooler opens up a possibility of DC flow circulating around the regenerator and the pulse tube. The DC flow sometimes deteriorates the performance of the cryocooler because such a steady flow adds an unwanted thermal load to the cold heat exchanger. It seems that this problem is still not well solved although a lot of effort has been made. Here we introduce a membrane-barrier method for DC flow suppression in double-inlet pulse tube coolers. An elastic membrane is installed between the pulse tube cooler inlet and the double-inlet valve to break the closed-loop flow path of DC flow. The membrane is acoustically transparent, but would block the DC flow completely. Thus the DC flow is thoroughly suppressed and the merit of double-inlet mode is remained. With this method, a temperature reduction of tens of Kelvin was obtained in our single-stage pulse tube cooler and the lowest temperature reached 29.8 K.
Application of the homotopy method for analytical solution of non-Newtonian channel flows
NASA Astrophysics Data System (ADS)
Roohi, Ehsan; Kharazmi, Shahab; Farjami, Yaghoub
2009-06-01
This paper presents the homotopy series solution of the Navier-Stokes and energy equations for non-Newtonian flows. Three different problems, Couette flow, Poiseuille flow and Couette-Poiseuille flow have been investigated. For all three cases, the nonlinear momentum and energy equations have been solved using the homotopy method and analytical approximations for the velocity and the temperature distribution have been obtained. The current results agree well with those obtained by the homotopy perturbation method derived by Siddiqui et al (2008 Chaos Solitons Fractals 36 182-92). In addition to providing analytical solutions, this paper draws attention to interesting physical phenomena observed in non-Newtonian channel flows. For example, it is observed that the velocity profile of non-Newtonian Couette flow is indistinctive from the velocity profile of the Newtonian one. Additionally, we observe flow separation in non-Newtonian Couette-Poiseuille flow even though the pressure gradient is negative (favorable). We provide physical reasoning for these unique phenomena.
Finite volume and finite element methods applied to 3D laminar and turbulent channel flows
Louda, Petr; Příhoda, Jaromír; Sváček, Petr; Kozel, Karel
2014-12-10
The work deals with numerical simulations of incompressible flow in channels with rectangular cross section. The rectangular cross section itself leads to development of various secondary flow patterns, where accuracy of simulation is influenced by numerical viscosity of the scheme and by turbulence modeling. In this work some developments of stabilized finite element method are presented. Its results are compared with those of an implicit finite volume method also described, in laminar and turbulent flows. It is shown that numerical viscosity can cause errors of same magnitude as different turbulence models. The finite volume method is also applied to 3D turbulent flow around backward facing step and good agreement with 3D experimental results is obtained.
Walker, Iain; Stratton, Chris
2015-08-01
This project improved the accuracy of air flow measurements used in commissioning California heating and air conditioning systems in Title 24 (Building and Appliance Efficiency Standards), thereby improving system performance and efficiency of California residences. The research team at Lawrence Berkeley National Laboratory addressed the issue that typical tools used by contractors in the field to test air flows may not be accurate enough to measure return flows used in Title 24 applications. The team developed guidance on performance of current diagnostics as well as a draft test method for use in future evaluations. The study team prepared a draft test method through ASTM International to determine the uncertainty of air flow measurements at residential heating ventilation and air conditioning returns and other terminals. This test method, when finalized, can be used by the Energy Commission and other entities to specify required accuracy of measurement devices used to show compliance with standards.
A spectral optical flow method for determining velocities from digital imagery
NASA Astrophysics Data System (ADS)
Hurlburt, Neal; Jaffey, Steve
2015-12-01
We present a method for determining surface flows from solar images based upon optical flow techniques. We apply the method to sets of images obtained by a variety of solar imagers to assess its performance. The opflow3d procedure is shown to extract accurate velocity estimates when provided perfect test data and quickly generates results consistent with completely distinct methods when applied on global scales. We also validate it in detail by comparing it to an established method when applied to high-resolution datasets and find that it provides comparable results without the need to tune, filter or otherwise preprocess the images before its application.
A pressure based method for the solution of viscous incompressible turbomachinery flows
NASA Technical Reports Server (NTRS)
Hobson, Garth Victor; Lakshminarayana, B.
1991-01-01
A new technique was developed for the solution of the incompressible Navier-Stokes equations. The numerical technique, derived from a pressure substitution method (PSM), overcomes many of the deficiencies of the pressure correction method. This technique allows for the direct solution of the actual pressure in the form of a Poisson equation which is derived from the pressure weighted substitution of the full momentum equations into the continuity equation. Two dimensional internal flows are computed with this method. The prediction of cascade performance is presented. The extention of the pressure correction method for the solution of three dimensional flows is also presented.
High fidelity digital inline holographic method for 3D flow measurements.
Toloui, Mostafa; Hong, Jiarong
2015-10-19
Among all the 3D optical flow diagnostic techniques, digital inline holographic particle tracking velocimetry (DIH-PTV) provides the highest spatial resolution with low cost, simple and compact optical setups. Despite these advantages, DIH-PTV suffers from major limitations including poor longitudinal resolution, human intervention (i.e. requirement for manually determined tuning parameters during tracer field reconstruction and extraction), limited tracer concentration, and expensive computations. These limitations prevent this technique from being widely used for high resolution 3D flow measurements. In this study, we present a novel holographic particle extraction method with the goal of overcoming all the major limitations of DIH-PTV. The proposed method consists of multiple steps involving 3D deconvolution, automatic signal-to-noise ratio enhancement and thresholding, and inverse iterative particle extraction. The entire method is implemented using GPU-based algorithm to increase the computational speed significantly. Validated with synthetic particle holograms, the proposed method can achieve particle extraction rate above 95% with fake particles less than 3% and maximum position error below 1.6 particle diameter for holograms with particle concentration above 3000 particles/mm^{3}. The applicability of the proposed method for DIH-PTV has been further validated using the experiment of laminar flow in a microchannel and the synthetic tracer flow fields generated using a DNS turbulent channel flow database. Such improvements will substantially enhance the implementation of DIH-PTV for 3D flow measurements and enable the potential commercialization of this technique. PMID:26480377
NASA Astrophysics Data System (ADS)
Arattano, M.; Abancó, C.; Coviello, V.; Hürlimann, M.
2014-12-01
Ground vibration sensors have been increasingly used and tested, during the last few years, as devices to monitor debris flows and they have also been proposed as one of the more reliable devices for the design of debris flow warning systems. The need to process the output of ground vibration sensors, to diminish the amount of data to be recorded, is usually due to the reduced storing capabilities and the limited power supply, normally provided by solar panels, available in the high mountain environment. There are different methods that can be found in literature to process the ground vibration signal produced by debris flows. In this paper we will discuss the two most commonly employed: the method of impulses and the method of amplitude. These two methods of data processing are analyzed describing their origin and their use, presenting examples of applications and their main advantages and shortcomings. The two methods are then applied to process the ground vibration raw data produced by a debris flow occurred in the Rebaixader Torrent (Spanish Pyrenees) in 2012. The results of this work will provide means for decision to researchers and technicians who find themselves facing the task of designing a debris flow monitoring installation or a debris flow warning equipment based on the use of ground vibration detectors.
Pedler, William H. (Radon Abatement Systems, Inc., Golden, CO); Jepsen, Richard Alan (Sandia National Laboratories, Carlsbad, NM)
2003-08-01
The requirement to accurately measure subsurface groundwater flow at contaminated sites, as part of a time and cost effective remediation program, has spawned a variety of flow evaluation technologies. Validation of the accuracy and knowledge regarding the limitations of these technologies are critical for data quality and application confidence. Leading the way in the effort to validate and better understand these methodologies, the US Army Environmental Center has funded a multi-year program to compare and evaluate all viable horizontal flow measurement technologies. This multi-year program has included a field comparison phase, an application of selected methods as part of an integrated site characterization program phase, and most recently, a laboratory and numerical simulator phase. As part of this most recent phase, numerical modeling predictions and laboratory measurements were made in a simulated fracture borehole set-up within a controlled flow simulator. The scanning colloidal borescope flowmeter (SCBFM) and advanced hydrophysical logging (NxHpL{trademark}) tool were used to measure velocities and flow rate in a simulated fractured borehole in the flow simulator. Particle tracking and mass flux measurements were observed and recorded under a range of flow conditions in the simulator. Numerical models were developed to aid in the design of the flow simulator and predict the flow conditions inside the borehole. Results demonstrated that the flow simulator allowed for predictable, easily controlled, and stable flow rates both inside and outside the well. The measurement tools agreed well with each other over a wide range of flow conditions. The model results demonstrate that the Scanning Colloidal Borescope did not interfere with the flow in the borehole in any of the tests. The model is capable of predicting flow conditions and agreed well with the measurements and observations in the flow simulator and borehole. Both laboratory and model results showed a
Lin, Chih-Ching; Chang, Chao-Fu; Chiou, Hong-Jen; Sun, Ying-Chou; Chiang, Shou-Shan; Lin, Ming-Wei; Lee, Pui-Ching; Yang, Wu-Chang
2005-01-01
Decreasing vascular access flow (Qa) is an important predictor of future access thrombosis and malfunction for hemodialysis (HD) patients. Among all of the methods for determining Qa, the variable pump flow (VPF) Doppler method measures Qa according to the change in Doppler signal between the arterial and the venous needles under different pump flow. After this technique was combined with spectral analysis of Duplex Doppler imaging, the variable pump flow-based Doppler ultrasound method (VPFDUM) for Qa measurement was developed. This study compared the reproducibility and correlation of Qa measurements for three different methods-VPFDUM, ultrasound dilution method (UDM), and conventional Doppler ultrasound method (CDUM)-in 55 HD patients. The mean value of Qa by VPFDUM (870.8 +/- 412.0 ml/min) was close to that by UDM (868.6 +/- 417.9 ml/min) but higher than that by CDUM (either of the above values versus 685.1 +/- 303.6 ml/min; P < 0.005). The mean values of coefficient of variation were similar by VPFDUM (1.6%) and UDM (1.4%) but lower than that by CDUM (either of the above values versus 6.8%; P < 0.01). The correlation coefficient and intraclass correlation coefficient of the repeated Qa measurements by VPFDUM (0.985 and 0.993; P < 0.001) were also similar to those by UDM (0.992 and 0.995; P < 0.001) but slightly higher than those by CDUM (0.917 and 0.948; P < 0.005). Either the reproducibility of VPFDUM (r=0.98, P < 0.0001) or the correlation between VPFDUM and UDM (r=0.99, P < 0.0001) in Qa measurements is good. The unassisted patency of vascular access at 6 mo was significantly poorer in patients with Qa <500 ml/min than those with Qa >500 ml/min (13.6% versus 92.2%; P < 0.0001). In conclusion, VPFDUM is a noninvasive, accurate, and reliable procedure for Qa measurement and prediction of the prognosis of vascular access in HD patients.
López-López, Alberto; Flores-Payán, Valentín; León-Becerril, Elizabeth; Hernández-Mena, Leonel; Vallejo-Rodríguez, Ramiro
2016-01-01
Steroids are classified as endocrine disrupting chemicals; they are persistent with low biodegradability and are hardly degraded by conventional methods. Ozonation process has been effective for steroids degradation and the determination of the kinetics is a fundamental aspect for the design and operation of the reactor. This study assessed two methods: competitive kinetics and stopped flow, for determining the degradation kinetics of two steroids, estradiol (E2) and ethinylestradiol (EE2) in spiked water. Experiments were performed at pH 6, 21 °C, and using tertbutyl alcohol as scavenger of hydroxyl radicals; competitive kinetics method used sodium phenolate as reference compound. For the stopped flow, the experiments were performed in a BioLogic SFM-3000/S equipment. For both methods, the second order rate constants were in the order of 10(6) and 10(5) M(-1) s(-1) for E2 and EE2 respectively. The competitive kinetics can be applied with assurance and reliability but needing an additional analysis method to measure the residual concentrations. Stopped flow method allows the evaluation of the degradation kinetics in milliseconds and avoids the use of additional analytical methodologies; this method allows determining the reaction times on line. The methods are applicable for degradation of other emerging contaminants or other steroids and could be applied in water treatment at industrial level. Finally, it is important to consider the resources available to implement the most appropriate method, either competitive kinetics or the stopped-flow method. PMID:27478722
Apparatus and method for acoustic monitoring of steam quality and flow
Sinha, Dipen N.; Pantea, Cristian
2016-09-13
An apparatus and method for noninvasively monitoring steam quality and flow and in pipes or conduits bearing flowing steam, are described. By measuring the acoustic vibrations generated in steam-carrying conduits by the flowing steam either by direct contact with the pipe or remotely thereto, converting the measured acoustic vibrations into a frequency spectrum characteristic of the natural resonance vibrations of the pipe, and monitoring the amplitude and/or the frequency of one or more chosen resonance frequencies, changes in the steam quality in the pipe are determined. The steam flow rate and the steam quality are inversely related, and changes in the steam flow rate are calculated from changes in the steam quality once suitable calibration curves are obtained.
NASA Technical Reports Server (NTRS)
Haviland, J. K.
1974-01-01
The results are reported of two unrelated studies. The first was an investigation of the formulation of the equations for non-uniform unsteady flows, by perturbation of an irrotational flow to obtain the linear Green's equation. The resulting integral equation was found to contain a kernel which could be expressed as the solution of the adjoint flow equation, a linear equation for small perturbations, but with non-constant coefficients determined by the steady flow conditions. It is believed that the non-uniform flow effects may prove important in transonic flutter, and that in such cases, the use of doublet type solutions of the wave equation would then prove to be erroneous. The second task covered an initial investigation into the use of the Monte Carlo method for solution of acoustical field problems. Computed results are given for a rectangular room problem, and for a problem involving a circular duct with a source located at the closed end.
NASA Technical Reports Server (NTRS)
Kim, Sang-Wook
1987-01-01
Various experimental, analytical, and numerical analysis methods for flow-solid interaction of a nest of cylinders subjected to cross flows are reviewed. A nest of cylinders subjected to cross flows can be found in numerous engineering applications including the Space Shuttle Maine Engine-Main Injector Assembly (SSME-MIA) and nuclear reactor heat exchangers. Despite its extreme importance in engineering applications, understanding of the flow-solid interaction process is quite limited and design of the tube banks are mostly dependent on experiments and/or experimental correlation equations. For future development of major numerical analysis methods for the flow-solid interaction of a nest of cylinders subjected to cross flow, various turbulence models, nonlinear structural dynamics, and existing laminar flow-solid interaction analysis methods are included.
A novel min-cost flow method for estimating transcript expression with RNA-Seq
2013-01-01
Background Through transcription and alternative splicing, a gene can be transcribed into different RNA sequences (isoforms), depending on the individual, on the tissue the cell is in, or in response to some stimuli. Recent RNA-Seq technology allows for new high-throughput ways for isoform identification and quantification based on short reads, and various methods have been put forward for this non-trivial problem. Results In this paper we propose a novel radically different method based on minimum-cost network flows. This has a two-fold advantage: on the one hand, it translates the problem as an established one in the field of network flows, which can be solved in polynomial time, with different existing solvers; on the other hand, it is general enough to encompass many of the previous proposals under the least sum of squares model. Our method works as follows: in order to find the transcripts which best explain, under a given fitness model, a splicing graph resulting from an RNA-Seq experiment, we find a min-cost flow in an offset flow network, under an equivalent cost model. Under very weak assumptions on the fitness model, the optimal flow can be computed in polynomial time. Parsimoniously splitting the flow back into few path transcripts can be done with any of the heuristics and approximations available from the theory of network flows. In the present implementation, we choose the simple strategy of repeatedly removing the heaviest path. Conclusions We proposed a new very general method based on network flows for a multiassembly problem arising from isoform identification and quantification with RNA-Seq. Experimental results on prediction accuracy show that our method is very competitive with popular tools such as Cufflinks and IsoLasso. Our tool, called Traph (Transcrips in gRAPHs), is available at: http://www.cs.helsinki.fi/gsa/traph/. PMID:23734627
Trace projection transformation: A new method for measurement of debris flow surface velocity fields
NASA Astrophysics Data System (ADS)
Yan, Yan; Cui, Peng; Guo, Xiaojun; Ge, Yonggang
2016-09-01
Spatiotemporal variation of velocity is important for debris flow dynamics. This paper presents a new method, the trace projection transformation, for accurate, non-contact measurement of a debris-flow surface velocity field based on a combination of dense optical flow and perspective projection transformation. The algorithm for interpreting and processing is implemented in C ++ and realized in Visual Studio 2012. The method allows quantitative analysis of flow motion through videos from various angles (camera positioned at the opposite direction of fluid motion). It yields the spatiotemporal distribution of surface velocity field at pixel level and thus provides a quantitative description of the surface processes. The trace projection transformation is superior to conventional measurement methods in that it obtains the full surface velocity field by computing the optical flow of all pixels. The result achieves a 90% accuracy of when comparing with the observed values. As a case study, the method is applied to the quantitative analysis of surface velocity field of a specific debris flow.
Nonequilibrium hypersonic flows simulations with asymptotic-preserving Monte Carlo methods
NASA Astrophysics Data System (ADS)
Ren, Wei; Liu, Hong; Jin, Shi
2014-12-01
In the rarefied gas dynamics, the DSMC method is one of the most popular numerical tools. It performs satisfactorily in simulating hypersonic flows surrounding re-entry vehicles and micro-/nano- flows. However, the computational cost is expensive, especially when Kn → 0. Even for flows in the near-continuum regime, pure DSMC simulations require a number of computational efforts for most cases. Albeit several DSMC/NS hybrid methods are proposed to deal with this, those methods still suffer from the boundary treatment, which may cause nonphysical solutions. Filbet and Jin [1] proposed a framework of new numerical methods of Boltzmann equation, called asymptotic preserving schemes, whose computational costs are affordable as Kn → 0. Recently, Ren et al. [2] realized the AP schemes with Monte Carlo methods (AP-DSMC), which have better performance than counterpart methods. In this paper, AP-DSMC is applied in simulating nonequilibrium hypersonic flows. Several numerical results are computed and analyzed to study the efficiency and capability of capturing complicated flow characteristics.
Range of valid arguments for data reduction method in the steam flow fields
NASA Astrophysics Data System (ADS)
Šafařík, Pavel; Nový, Adam; Hajšman, Miroslav; Jícha, David
2014-08-01
The data reduction method is based on conservation laws and has been developed for an ideal gas. The method is widely adopted in the aerodynamic experimental research. The presented paper shows an attempt to extend this method for steam flows. Range of valid arguments in the data reduction method is determined by means of numerical calculations using the IAPWS-IF97 equations of state of steam.
Alva, Carlos; Gómez, Felipe David; Ortegón Cardeña, José; Yáñez Gutiérrez, Lucelli; Lazcano, Sonia
2008-01-01
In patients with Bidirectional Glenn who have undergone surgery, the superior caval venous flow provides the only pulmonary blood supply. This is the effective pulmonary flow and at the same time its volume is not enough to overflow the single ventricle. The unsaturated, inferior vena cava flow is not oxygenated, since it goes across the interatrial septal communication and gets mixed in the left ventricle with the pulmonary venous blood. In this work, a bidirectional Glenn case is analyzed. The hemodynamic data before and after the operation are shown. It was evident from this case that the use of the Fick method to measure pulmonary flow in patients with bidirectional Glenn operation is not appropriate. Alternative methods, such as Doppler echocardiography and Magnetic Resonance Imaging, are recommended. A literature review on this subject was carefully done.
Borazjani, Iman; Westerdale, John; McMahon, Eileen M.; Rajaraman, Prathish K.; Heys, Jeffrey J.
2013-01-01
The left ventricle (LV) pumps oxygenated blood from the lungs to the rest of the body through systemic circulation. The efficiency of such a pumping function is dependent on blood flow within the LV chamber. It is therefore crucial to accurately characterize LV hemodynamics. Improved understanding of LV hemodynamics is expected to provide important clinical diagnostic and prognostic information. We review the recent advances in numerical and experimental methods for characterizing LV flows and focus on analysis of intraventricular flow fields by echocardiographic particle image velocimetry (echo-PIV), due to its potential for broad and practical utility. Future research directions to advance patient-specific LV simulations include development of methods capable of resolving heart valves, higher temporal resolution, automated generation of three-dimensional (3D) geometry, and incorporating actual flow measurements into the numerical solution of the 3D cardiovascular fluid dynamics. PMID:23690874
Material point method of modelling and simulation of reacting flow of oxygen
NASA Astrophysics Data System (ADS)
Mason, Matthew; Chen, Kuan; Hu, Patrick G.
2014-07-01
Aerospace vehicles are continually being designed to sustain flight at higher speeds and higher altitudes than previously attainable. At hypersonic speeds, gases within a flow begin to chemically react and the fluid's physical properties are modified. It is desirable to model these effects within the Material Point Method (MPM). The MPM is a combined Eulerian-Lagrangian particle-based solver that calculates the physical properties of individual particles and uses a background grid for information storage and exchange. This study introduces chemically reacting flow modelling within the MPM numerical algorithm and illustrates a simple application using the AeroElastic Material Point Method (AEMPM) code. The governing equations of reacting flows are introduced and their direct application within an MPM code is discussed. A flow of 100% oxygen is illustrated and the results are compared with independently developed computational non-equilibrium algorithms. Observed trends agree well with results from an independently developed source.
Sjogreen, B; Yee, H C
2007-12-12
Flows containing steady or nearly steady strong shocks in parts of the flow field, and unsteady turbulence with shocklets on other parts of the flow field are difficult to capture accurately and efficiently employing the same numerical scheme even under the multiblock grid or adaptive grid refinement framework. On one hand, sixth-order or higher shock-capturing methods are appropriate for unsteady turbulence with shocklets. On the other hand, lower order shock-capturing methods are more effective for strong steady shocks in terms of convergence. In order to minimize the shortcomings of low order and high order shock-capturing schemes for the subject flows, a multi-block overlapping grid with different orders of accuracy on different blocks is proposed. Test cases to illustrate the performance of the new solver are included.
Implicit method for the computation of unsteady flows on unstructured grids
NASA Technical Reports Server (NTRS)
Venkatakrishnan, V.; Mavriplis, D. J.
1995-01-01
An implicit method for the computation of unsteady flows on unstructured grids is presented. Following a finite difference approximation for the time derivative, the resulting nonlinear system of equations is solved at each time step by using an agglomeration multigrid procedure. The method allows for arbitrarily large time steps and is efficient in terms of computational effort and storage. Inviscid and viscous unsteady flows are computed to validate the procedure. The issue of the mass matrix which arises with vertex-centered finite volume schemes is addressed. The present formulation allows the mass matrix to be inverted indirectly. A mesh point movement and reconnection procedure is described that allows the grids to evolve with the motion of bodies. As an example of flow over bodies in relative motion, flow over a multi-element airfoil system undergoing deployment is computed.
Implicit method for the computation of unsteady flows on unstructured grids
Venkatakrishnan, V.; Mavriplis, D.J.
1996-09-01
An implicit method for the computation of unsteady flows on unstructured grids is presented. Following a finite difference approximation for the time derivative, the resulting nonlinear system of equations is solved at each time step by using an agglomeration multigrid procedure. The method allows for arbitrarily large time steps and is efficient in terms of computational effort and storage. Inviscid and viscous unsteady flows are computed to validate the procedure. The issue of the mass matrix which arises with vertex-centered finite volume schemes is addressed. The present formulation allows the mass matrix to be inverted indirectly. A mesh point movement and reconnection procedure is described that allows the grids to evolve with the motion of bodies. As an example of flow over bodies in relative motion, flow over a multi-element airfoil system undergoing deployment is computed. 48 refs., 24 figs.
Method and apparatus for measuring mass flow and energy content using a differential pressure meter
Vander Heyden, W.H.
1993-07-13
An apparatus is described for measuring a ratio of a mass flow rate of a pipeline gas flowing through a pipeline compared to a mass flow rate of a sample gas tapped from the pipeline, the pipeline having a first device means for producing a pipeline gas pressure differential, the apparatus comprising: a second device means for producing a sample gas pressure differential while maintaining the sample gas at substantially the same temperature as the pipeline gas; means for routing the sample gas to the second device means; means for measuring the pipeline gas pressure differential as the pipeline gas flows through the first device means; means for measuring the sample gas pressure differential as the sample gas flows through the second device means; a flow controlling means located downstream of the second device means for maintaining a sample gas flow rate through the second device means independent of the pipeline gas pressure differential; and a control means for comparing the sample gas pressure differential with the pipeline gas pressure differential in order to obtain the mass flow rate ratio. A method is described for using the above apparatus.
A method for obtaining a statistically stationary turbulent free shear flow
NASA Technical Reports Server (NTRS)
Timson, Stephen F.; Lele, S. K.; Moser, R. D.
1994-01-01
The long-term goal of the current research is the study of Large-Eddy Simulation (LES) as a tool for aeroacoustics. New algorithms and developments in computer hardware are making possible a new generation of tools for aeroacoustic predictions, which rely on the physics of the flow rather than empirical knowledge. LES, in conjunction with an acoustic analogy, holds the promise of predicting the statistics of noise radiated to the far-field of a turbulent flow. LES's predictive ability will be tested through extensive comparison of acoustic predictions based on a Direct Numerical Simulation (DNS) and LES of the same flow, as well as a priori testing of DNS results. The method presented here is aimed at allowing simulation of a turbulent flow field that is both simple and amenable to acoustic predictions. A free shear flow is homogeneous in both the streamwise and spanwise directions and which is statistically stationary will be simulated using equations based on the Navier-Stokes equations with a small number of added terms. Studying a free shear flow eliminates the need to consider flow-surface interactions as an acoustic source. The homogeneous directions and the flow's statistically stationary nature greatly simplify the application of an acoustic analogy.
The Blow Up Method for Brakke Flows: Networks Near Triple Junctions
NASA Astrophysics Data System (ADS)
Tonegawa, Yoshihiro; Wickramasekera, Neshan
2016-09-01
We introduce a parabolic blow-up method to study the asymptotic behavior of a Brakke flow of planar networks (that is a 1-dimensional Brakke flow in a two dimensional region) weakly close in a space-time region to a static multiplicity 1 triple junction J. We show that such a network flow is regular in a smaller space-time region, in the sense that it consists of three curves coming smoothly together at a single point at 120{^{circ}} angles, staying smoothly close to J and moving smoothly. Using this result and White's stratification theorem, we deduce that whenever a Brakke flow of networks in a space-time region {{mathcal {R}}} has no static tangent flow with density {{≥q}2}, there exists a closed subset {{Σ subset {mathcal {R}}}} of parabolic Hausdorff dimension at most 1 such that the flow is classical in {{mathcal {R}}backslashΣ}, that is near every point in {{mathcal {R}}backslashΣ}, the flow, if non-empty, consists of either an embedded curve moving smoothly or three embedded curves meeting smoothly at a single point at 120{^{circ}} angles and moving smoothly. In particular, such a flow is classical at all times except for a closed set of times of ordinary Hausdorff dimension at most {1/2}.
NASA Astrophysics Data System (ADS)
Tsuchiya, Takenobu; Hatano, Yuichi; Mori, Yashunori; Shen, Rakushin; Endoh, Nobuyuki
2016-07-01
In this study, to estimate the local temperature changes caused by a thick blood vessel, the temperature distribution in a tissue phantom with a thick blood vessel during focused ultrasound irradiation was measured by a thermal imaging method. The blood flow rate in the simulated blood vessel was varied and the relationship between flow rate and temperature decrease was examined. The phantom using the thermal imaging method is divided into two parts, and the increases in temperature distribution as a function of blood flow rate are measured using a thermocamera under constant ultrasound irradiation. The irradiation conditions of ultrasound waves were a central frequency of 1 MHz, a wave number length of 200 cycles, and a duty ratio of 0.2. The irradiation duration was 5 min, and the ultrasound intensity I SPTA was 36 W/cm2. The amount of temperature decrease caused by the cooling effect of blood flow increased with the blood flow rate and it became constant at a certain threshold of blood flow rate. The threshold of blood flow rate is about 250 ml/min.
Research on optical fiber flow test method with non-intrusion
NASA Astrophysics Data System (ADS)
Shang, Ying; Liu, Xiaohui; Wang, Chang; Zhao, Wenan
2014-06-01
In the field of oil well logging, real-time monitoring of the fluid flow parameter provides a scientific basis for oil and gas optimization exploration and increase in reservoir recovery, so a non-intrusive flow test method based on turbulent vibration was proposed. The specific length of the sensor fiber wound tightly around the outer wall of the pipe was connected with the optical fiber gratings at both ends, and the sensor fiber and the optical fiber gratings composed the flow sensing unit. The dynamic pressure was generated by the turbulence when fluid flows through the pipe, and the dynamic pressure resulted in the light phase shift of the sensor fiber. The phase information was demodulated by the fiber optic interferometer technology, time division multiplexing technology, and phase generated carrier modulation and demodulation techniques. The quadratic curve relationship between the phase change and flow rate was found by experimental data analysis, and the experiment confirmed the feasibility of the optical fiber flow test method with non-intrusion and achieved the real-time monitoring of the fluid flow.
NASA Astrophysics Data System (ADS)
Leclaire, S.; Pellerin, N.; Reggio, M.; Trépanier, J.-Y.
2014-03-01
The lattice Boltzmann modeling of immiscible multiphase flows needs to be further validated, especially when density variation occurs between the different flow phases. From this perspective, the goal of this research is to introduce the multiple-relaxation-time operator into a lattice Boltzmann model in order to improve its numerical stability in the presence of large density and viscosity ratios. Essentially, this research shows that the introduction of this operator greatly improves the numerical stability of the approach compared to the original single-relaxation-time collision operator. In many lattice Boltzmann research studies, multiphase lattice Boltzmann methods are validated using a reduced number of test cases, and unsteady flow test cases are frequently omitted before much more complex flow configurations are simulated. In this context, several test cases are proposed to evaluate the behavior of a lattice Boltzmann method for simulating immiscible multiphase flows with high density and viscosity ratios. These are: (1) two-phase Couette flow; (2) three-phase Laplace law; (3) three-phase Zalesak disk; (4) two-phase flow between oscillating plates; (5) two-phase capillary wave; and (6) the two-phase oscillating cylindrical bubble. The first two involve a steady regime, and the remaining four an unsteady regime.
A High-Resolution Godunov Method for Compressible Multi-Material Flow on Overlapping Grids
Banks, J W; Schwendeman, D W; Kapila, A K; Henshaw, W D
2006-02-13
A numerical method is described for inviscid, compressible, multi-material flow in two space dimensions. The flow is governed by the multi-material Euler equations with a general mixture equation of state. Composite overlapping grids are used to handle complex flow geometry and block-structured adaptive mesh refinement (AMR) is used to locally increase grid resolution near shocks and material interfaces. The discretization of the governing equations is based on a high-resolution Godunov method, but includes an energy correction designed to suppress numerical errors that develop near a material interface for standard, conservative shock-capturing schemes. The energy correction is constructed based on a uniform pressure-velocity flow and is significant only near the captured interface. A variety of two-material flows are presented to verify the accuracy of the numerical approach and to illustrate its use. These flows assume an equation of state for the mixture based on Jones-Wilkins-Lee (JWL) forms for the components. This equation of state includes a mixture of ideal gases as a special case. Flow problems considered include unsteady one-dimensional shock-interface collision, steady interaction of an planar interface and an oblique shock, planar shock interaction with a collection of gas-filled cylindrical inhomogeneities, and the impulsive motion of the two-component mixture in a rigid cylindrical vessel.
A Method for the Constrained Design of Natural Laminar Flow Airfoils
NASA Technical Reports Server (NTRS)
Green, Bradford E.; Whitesides, John L.; Campbell, Richard L.; Mineck, Raymond E.
1996-01-01
A fully automated iterative design method has been developed by which an airfoil with a substantial amount of natural laminar flow can be designed, while maintaining other aerodynamic and geometric constraints. Drag reductions have been realized using the design method over a range of Mach numbers, Reynolds numbers and airfoil thicknesses. The thrusts of the method are its ability to calculate a target N-Factor distribution that forces the flow to undergo transition at the desired location; the target-pressure-N-Factor relationship that is used to reduce the N-Factors in order to prolong transition; and its ability to design airfoils to meet lift, pitching moment, thickness and leading-edge radius constraints while also being able to meet the natural laminar flow constraint. The method uses several existing CFD codes and can design a new airfoil in only a few days using a Silicon Graphics IRIS workstation.
On the accuracy of Whitham's method. [for steady ideal gas flow past cones
NASA Technical Reports Server (NTRS)
Zahalak, G. I.; Myers, M. K.
1974-01-01
The steady flow of an ideal gas past a conical body is studied by the method of matched asymptotic expansions and by Whitham's method in order to assess the accuracy of the latter. It is found that while Whitham's method does not yield a correct asymptotic representation of the perturbation field to second order in regions where the flow ahead of the Mach cone of the apex is disturbed, it does correctly predict the changes of the second-order perturbation quantities across a shock (the first-order shock strength). The results of the analysis are illustrated by a special case of a flat, rectangular plate at incidence.
Applications of the Lattice Boltzmann Method to Complex and Turbulent Flows
NASA Technical Reports Server (NTRS)
Luo, Li-Shi; Qi, Dewei; Wang, Lian-Ping; Bushnell, Dennis M. (Technical Monitor)
2002-01-01
We briefly review the method of the lattice Boltzmann equation (LBE). We show the three-dimensional LBE simulation results for a non-spherical particle in Couette flow and 16 particles in sedimentation in fluid. We compare the LBE simulation of the three-dimensional homogeneous isotropic turbulence flow in a periodic cubic box of the size 1283 with the pseudo-spectral simulation, and find that the two results agree well with each other but the LBE method is more dissipative than the pseudo-spectral method in small scales, as expected.
Computations of Flow Over the Hump Model Using Higher-Order Method With Turbulence Modeling
NASA Technical Reports Server (NTRS)
Balakumar, P.
2007-01-01
The flow over the two-dimensional hump model is computed by solving the RANS equations with kappa-omega (SST) model. The governing equations, the flow equations and the turbulent equations, are solved using the 5th order accurate weighted essentially non-oscillatory (WENO) scheme for space discretization and using explicit third order total-variation-diminishing (TVD) Runge-Kutta scheme for time integration. The WENO and the TVD methods and the formulas are explained in [1] and the application of ENO method to N-S equations is given in [2]. The solution method implemented in this computation is described in detail in [3].
Synthesis of zinc ultrafine powders via the Guen–Miller flow-levitation method
Jigatch, A. N. Leipunskii, I. O.; Kuskov, M. L.; Afanasenkova, E. S.; Berezkina, N. G.; Gorbatov, S. A.
2015-12-15
Zinc ultrafine powders (UFPs) with the average particle size of 0.175 to 1.24 μm are synthesized via the flow-levitation method. The peculiarities of the formation of zinc UFPs are considered with respect to the carrier gas properties (heat capacity, thermal conductivity, and diffusion coefficient), as well as the gas flow parameters (pressure and flow rate). The obtained zinc particles are studied via scanning electron microscopy and X-ray diffraction. The factors determining the crystal structure of zinc particles and their size distribution are discussed as well. The data on oxidation of zinc stored in unsealed containers under normal conditions are also presented.
An analysis method for multi-component airfoils in separated flow
NASA Technical Reports Server (NTRS)
Rao, B. M.; Duorak, F. A.; Maskew, B.
1980-01-01
The multi-component airfoil program (Langley-MCARF) for attached flow is modified to accept the free vortex sheet separation-flow model program (Analytical Methods, Inc.-CLMAX). The viscous effects are incorporated into the calculation by representing the boundary layer displacement thickness with an appropriate source distribution. The separation flow model incorporated into MCARF was applied to single component airfoils. Calculated pressure distributions for angles of attack up to the stall are in close agreement with experimental measurements. Even at higher angles of attack beyond the stall, correct trends of separation, decrease in lift coefficients, and increase in pitching moment coefficients are predicted.
TVD-interpolating matrix method prediction of flow with heat transfer
NASA Astrophysics Data System (ADS)
Hanich, L.; Louaked, M.
2004-01-01
The use of the Interpolating Matrix Method to investigate the dynamical behaviour of natural convection flow will form the main focus of the work. We design an original and efficient numerical algorithm that mimic TVD constraints to rule out spurious overshoots and oscillations that appear near steep gradients, to solve fluid flow and heat transfer equations, and compare the results to some Benchmark numerical solutions.
Development of the Assessment Items of Debris Flow Using the Delphi Method
NASA Astrophysics Data System (ADS)
Byun, Yosep; Seong, Joohyun; Kim, Mingi; Park, Kyunghan; Yoon, Hyungkoo
2016-04-01
In recent years in Korea, Typhoon and the localized extreme rainfall caused by the abnormal climate has increased. Accordingly, debris flow is becoming one of the most dangerous natural disaster. This study aimed to develop the assessment items which can be used for conducting damage investigation of debris flow. Delphi method was applied to classify the realms of assessment items. As a result, 29 assessment items which can be classified into 6 groups were determined.
LABORATORY EVALUATION OF AIR FLOW MEASUREMENT METHODS FOR RESIDENTIAL HVAC RETURNS
Walker, Iain; Stratton, Chris
2015-02-01
This project improved the accuracy of air flow measurements used in commissioning California heating and air conditioning systems in Title 24 (Building and Appliance Efficiency Standards), thereby improving system performance and efficiency of California residences. The research team at Lawrence Berkeley National Laboratory addressed the issue that typical tools used by contractors in the field to test air flows may not be accurate enough to measure return flows used in Title 24 applications. The team developed guidance on performance of current diagnostics as well as a draft test method for use in future evaluations. The series of tests performed measured air flow using a range of techniques and devices. The measured air flows were compared to reference air flow measurements using inline air flow meters built into the test apparatus. The experimental results showed that some devices had reasonable results (typical errors of 5 percent or less) but others had much bigger errors (up to 25 percent). Because manufacturers’ accuracy estimates for their equipment do not include many of the sources of error found in actual field measurements (and replicated in the laboratory testing in this study) it is essential for a test method that could be used to determine the actual uncertainty in this specific application. The study team prepared a draft test method through ASTM International to determine the uncertainty of air flow measurements at residential heating ventilation and air conditioning returns and other terminals. This test method, when finalized, can be used by the Energy Commission and other entities to specify required accuracy of measurement devices used to show compliance with standards.
Method and apparatus to measure vapor pressure in a flow system
Grossman, M.W.; Biblarz, O.
1991-10-15
The present invention is directed to a method for determining, by a condensation method, the vapor pressure of a material with a known vapor pressure versus temperature characteristic, in a flow system particularly in a mercury isotope enrichment process. 2 figures.
A finite element method for the computation of transonic flow past airfoils
NASA Technical Reports Server (NTRS)
Eberle, A.
1980-01-01
A finite element method for the computation of the transonic flow with shocks past airfoils is presented using the artificial viscosity concept for the local supersonic regime. Generally, the classic element types do not meet the accuracy requirements of advanced numerical aerodynamics requiring special attention to the choice of an appropriate element. A series of computed pressure distributions exhibits the usefulness of the method.
NASA Astrophysics Data System (ADS)
Im, Dong-Kyun; Choi, Seongim; Hyuck Kwon, Jang
2015-01-01
The diagonally implicit harmonic balance method is developed in an overset mesh topology and applied to unsteady rotor flows analysis. Its efficiency is by reducing the complexity of a fully implicit harmonic balance method which becomes more flexible in handling the higher harmonics of the flow solutions. Applied to the overset mesh topology, the efficiency of the method becomes greater by reducing the number of solution interpolations required during the entire solution procedure as the method reduces the unsteady computation into periodic steady state. To verify the accuracy and efficiency of the method, both hovering and unsteady forward flight of Caradonna and Tung and AH-1G rotors are solved. Compared with wind-tunnel experiments, the numerical results demonstrate good agreements at computational cost an order of magnitude more efficient than the conventional time-accurate computation method. The proposed method has great potential in other engineering applications, including flapping wing vehicles, turbo-machinery, wind-turbines, etc.
The Investigation of Ghost Fluid Method for Simulating the Compressible Two-Medium Flow
NASA Astrophysics Data System (ADS)
Lu, Hai Tian; Zhao, Ning; Wang, Donghong
2016-06-01
In this paper, we investigate the conservation error of the two-dimensional compressible two-medium flow simulated by the front tracking method. As the improved versions of the original ghost fluid method, the modified ghost fluid method and the real ghost fluid method are selected to define the interface boundary conditions, respectively, to show different effects on the conservation error. A Riemann problem is constructed along the normal direction of the interface in the front tracking method, with the goal of obtaining an efficient procedure to track the explicit sharp interface precisely. The corresponding Riemann solutions are also used directly in these improved ghost fluid methods. Extensive numerical examples including the sod tube and the shock-bubble interaction are tested to calculate the conservation error. It is found that these two ghost fluid methods have distinctive performances for different initial conditions of the flow field, and the related conclusions are made to suggest the best choice for the combination.
NASA Technical Reports Server (NTRS)
Wong, T. C.; Tiwari, S. N.
1984-01-01
The aerodynamic characteristics of potential flow past an axisymmetric slender body and a thin airfoil are calculated using a uniform perturbation analysis method. The method is based on the superposition of potentials of point singularities distributed inside the body. The strength distribution satisfies a linear integral equation by enforcing the flow tangency condition on the surface of the body. The complete uniform asymptotic expansion of its solution is obtained with respect to the slenderness ratio by modifying and adapting an existing technique. Results calculated by the perturbation analysis method are compared with the existing surface singularity panel method and some available analytical solutions for a number of cases under identical conditions. From these comparisons, it is found that the perturbation analysis method can provide quite accurate results for bodies with small slenderness ratio. The present method is much simpler and requires less memory and computation time than existing surface singularity panel methods of comparable accuracy.
Suk, Heejun
2012-01-01
Abstract In articles published in 2009 and 2010, Suk and Yeh reported the development of an accurate and efficient particle tracking algorithm for simulating a path line under complicated unsteady flow conditions, using a range of elements within finite elements in multidimensions. Here two examples, an aquifer storage and recovery (ASR) example and a landfill leachate migration example, are examined to enhance the practical implementation of the proposed particle tracking method, known as Suk's method, to a real field of groundwater flow and transport. Results obtained by Suk's method are compared with those obtained by Pollock's method. Suk's method produces superior tracking accuracy, which suggests that Suk's method can describe more accurately various advection-dominated transport problems in a real field than existing popular particle tracking methods, such as Pollock's method. To illustrate the wide and practical applicability of Suk's method to random-walk particle tracking (RWPT), the original RWPT has been modified to incorporate Suk's method. Performance of the modified RWPT using Suk's method is compared with the original RWPT scheme by examining the concentration distributions obtained by the modified RWPT and the original RWPT under complicated transient flow systems. PMID:22476629
Variable parameter McCarthy-Muskingum routing method considering lateral flow
NASA Astrophysics Data System (ADS)
Yadav, Basant; Perumal, Muthiah; Bardossy, Andras
2015-04-01
The fully mass conservative variable parameter McCarthy-Muskingum (VPMM) method recently proposed by Perumal and Price (2013) for routing floods in channels and rivers without considering lateral flow is extended herein for accounting uniformly distributed lateral flow contribution along the reach. The proposed procedure is applied for studying flood wave movement in a 24.2 km river stretch between Rottweil and Oberndorf gauging stations of Neckar River in Germany wherein significant lateral flow contribution by intermediate catchment rainfall prevails during flood wave movement. The geometrical elements of the cross-sectional information of the considered routing river stretch without considering lateral flow are estimated using the Robust Parameter Estimation (ROPE) algorithm that allows for arriving at the best performing set of bed width and side slope of a trapezoidal section. The performance of the VPMM method is evaluated using the Nash-Sutcliffe model efficiency criterion as the objective function to be maximized using the ROPE algorithm. The twenty-seven flood events in the calibration set are considered to identify the relationship between 'total rainfall' and 'total losses' as well as to optimize the geometric characteristics of the prismatic channel (width and slope of the trapezoidal section). Based on this analysis, a relationship between total rainfall and total loss of the intermediate catchment is obtained and then used to estimate the lateral flow in the reach. Assuming the lateral flow hydrograph is of the form of inflow hydrograph and using the total intervening catchment runoff estimated from the relationship, the uniformly distributed lateral flow rate qL at any instant of time is estimated for its use in the VPMM routing method. All the 27 flood events are simulated using this routing approach considering lateral flow along the reach. Many of these simulations are able to simulate the observed hydrographs very closely. The proposed approach
Quick Analysis Method for Estimating Debris Flow Prone Area Caused by Overflow from Landslide dam
NASA Astrophysics Data System (ADS)
Shimizu, T.; Uchida, T.; Yamakoshi, T.; Yoshino, K.; Kisa, H.; Ishizuka, T.; Kaji, A.
2012-04-01
When earthquake or torrential rainfall cause deep catastrophic landslides, landslide dams can be formed in mountainous region. If water overflows from the landslide dams, large scale debris flow can occurs and possibly causes serious disasters in the downward region. Debris flow caused by the overflow from landslide dam is possible to affect the larger area than normal debris flow and flash flood. It is important for both a decision maker and resident in the area to recognize the disaster prone area as early as possible. For that reason, it is important to establish a quick analysis method for estimating debris flow prone area caused by overflow from landslide dams under the emergency situation. This situation requires the method to have both accuracy and speed for release. Nonetheless these two factors have trade-off relationship. We recently developed the quick analysis method to estimate debris flow disaster prone area caused by overflow from landslide dams. The method including the ways of efficient survey and numerical simulation programs called QUAD-L (QUick Analysis system for Debris flow caused by Landslide dam overflow). Our quick analysis system was actually applied to show the area for evacuation against debris flow caused by overflow from landslide dam formed by the 2011 Typhoon Talas which hit mainly the central region of Japan on September 2-4th, 2011. In addition to background of this application, since May 1st, 2011, Erosion and Sediment Control (SABO) Department of the Ministry of Land, Infrastructure, Transport and Tourism, Japan (MLIT) launched a new scheme using above-mentioned quick analysis method.
Radiation-transport method to simulate noncontinuum gas flows for MEMS devices.
Gallis, Michail A.; Torczynski, John Robert
2004-01-01
A Micro Electro Mechanical System (MEMS) typically consists of micron-scale parts that move through a gas at atmospheric or reduced pressure. In this situation, the gas-molecule mean free path is comparable to the geometric features of the microsystem, so the gas flow is noncontinuum. When mean-free-path effects cannot be neglected, the Boltzmann equation must be used to describe the gas flow. Solution of the Boltzmann equation is difficult even for the simplest case because of its sevenfold dimensionality (one temporal dimension, three spatial dimensions, and three velocity dimensions) and because of the integral nature of the collision term. The Direct Simulation Monte Carlo (DSMC) method is the method of choice to simulate high-speed noncontinuum flows. However, since DSMC uses computational molecules to represent the gas, the inherent statistical noise must be minimized by sampling large numbers of molecules. Since typical microsystem velocities are low (< 1 m/s) compared to molecular velocities ({approx}400 m/s), the number of molecular samples required to achieve 1% precision can exceed 1010 per cell. The Discrete Velocity Gas (DVG) method, an approach motivated by radiation transport, provides another way to simulate noncontinuum gas flows. Unlike DSMC, the DVG method restricts molecular velocities to have only certain discrete values. The transport of the number density of a velocity state is governed by a discrete Boltzmann equation that has one temporal dimension and three spatial dimensions and a polynomial collision term. Specification and implementation of DVG models are discussed, and DVG models are applied to Couette flow and to Fourier flow. While the DVG results for these benchmark problems are qualitatively correct, the errors in the shear stress and the heat flux can be order-unity even for DVG models with 88 velocity states. It is concluded that the DVG method, as described herein, is not sufficiently accurate to simulate the low-speed gas flows
Use of self-potential (SP) method to understand the regional groundwater flow system
NASA Astrophysics Data System (ADS)
Satou, S.; Shimada, J.; Goto, T.
2005-12-01
The self-potential method (SP method) is one of the geophysical explorations technique originally used to explore the mineral deposit for mining purposes. Recently, this technique has been applied to understand the geothermal fluid flow in and around the volcanic area. As there exists various factors to affect the surface SP measurement, it is rather difficult to find out the major cause of self-potential generation because their complexity of the generation mechanism. In this application, the behavior of groundwater flow is thought to be as a kind of noise. However, in case of non geothermal area, groundwater flow flux should create substantial self potential at the area which is less complex than geothermal area. The self-potential created by the groundwater flow is mainly caused by the streaming potential represented by the electrokinetic factors such as groundwater potential and the ground resistivity (Ishido and Mizutani,1981). As there exist little SP study to understand groundwater flow system, we have conducted the field SP measurement and its numerical model consideration in the clear groundwater flow existing area. A basin scale groundwater flow region including the mountainous ridge to the coastal area within one river-water catchment basin, which is geologically composed by the volcanic lava and tuff-breccia bedrock, was selected to apply the SP method_DThe study area is Shiranui town, Kumamoto, Kyusyu, Japan. In this area, following multi-hydrological studies have been conducted to understand the groundwater flow regime of the area: groundwater flow system study with observation boreholes and eivironmental isotopes, hydrometric observation for river discharge and precipitation for the regional water budget, micro-meteorological observation at different vegetation and altitude for the evapotranspiration measurement, submarine groundwater discharge investigation, geological borehole drilling, and 3D groundwater flow simulation, etc. The SP measurement
Comparison of PDF and Moment Closure Methods in the Modeling of Turbulent Reacting Flows
NASA Technical Reports Server (NTRS)
Norris, Andrew T.; Hsu, Andrew T.
1994-01-01
In modeling turbulent reactive flows, Probability Density Function (PDF) methods have an advantage over the more traditional moment closure schemes in that the PDF formulation treats the chemical reaction source terms exactly, while moment closure methods are required to model the mean reaction rate. The common model used is the laminar chemistry approximation, where the effects of turbulence on the reaction are assumed negligible. For flows with low turbulence levels and fast chemistry, the difference between the two methods can be expected to be small. However for flows with finite rate chemistry and high turbulence levels, significant errors can be expected in the moment closure method. In this paper, the ability of the PDF method and the moment closure scheme to accurately model a turbulent reacting flow is tested. To accomplish this, both schemes were used to model a CO/H2/N2- air piloted diffusion flame near extinction. Identical thermochemistry, turbulence models, initial conditions and boundary conditions are employed to ensure a consistent comparison can be made. The results of the two methods are compared to experimental data as well as to each other. The comparison reveals that the PDF method provides good agreement with the experimental data, while the moment closure scheme incorrectly shows a broad, laminar-like flame structure.
Multi-wave ultrasonic Doppler method for measuring high flow-rates using staggered pulse intervals
NASA Astrophysics Data System (ADS)
Muramatsu, Ei; Murakawa, Hideki; Sugimoto, Katsumi; Asano, Hitoshi; Takenaka, Nobuyuki; Furuichi, Noriyuki
2016-02-01
The ultrasonic pulsed Doppler method (UDM) can obtain a velocity profile along the path of an ultrasonic beam. However, the UDM measurement volume is relatively large and it is known that the measurement volume affects the measurement accuracy. In this study, the effect of the measurement volume on velocity and flow rate measurements is analytically and experimentally evaluated. The velocities measured using UDM are considered to be ensemble-averaged values over the measurement volume in order to analyze the velocity error due to the measurement volume, while the flow rates are calculated from the integration of the velocity profile across the pipe. The analytical results show that the channel width, i.e. the spatial resolution along the ultrasonic beam axis, rather than the ultrasonic beam diameter, strongly influences the flow rate measurement. To improve the accuracy of the flow rate, a novel method using a multi-wave ultrasonic transducer consisting of two piezo-electric elements with different basic frequencies is proposed to minimize the size of the measurement volume in the near-wall region of a pipe flow. The velocity profiles in the near-wall region are measured using an 8 MHz sensor with a small diameter, while those far from the transducer are measured using a hollow 2 MHz sensor in the multi-wave transducer. The applicability of the multi-wave transducer was experimentally investigated using the water flow-rate calibration facility at the National Institute of Advanced Industrial Science and Technology (AIST). As a result, the errors in the flow rate were found to be below -1%, while the multi-wave method is shown to be particularly effective for measuring higher flow rates in a large-diameter pipe.
Debris flow impact on mitigation barriers: a new method for particle-fluid-structure interactions
NASA Astrophysics Data System (ADS)
Marchelli, Maddalena; Pirulli, Marina; Pudasaini, Shiva P.
2016-04-01
Channelized debris-flows are a type of mass movements that involve water-charged, predominantly coarse-grained inorganic and organic material flowing rapidly down steep confined pre-existing channels (Van Dine, 1985). Due to their rapid movements and destructive power, structural mitigation measures have become an integral part of counter measures against these phenomena, to mitigate and prevent damages resulting from debris-flow impact on urbanized areas. In particular, debris barriers and storage basins, with some form of debris-straining structures incorporated into the barrier constructed across the path of a debris-flow, have a dual role to play: (1) to stimulate deposition by presenting a physical obstruction against flow, and (2) to guarantee that during normal conditions stream water and bedload can pass through the structure; while, during and after an extreme event, the water that is in the flow and some of the fine-grained sediment can escape. A new method to investigate the dynamic interactions between the flowing mass and the debris barrier is presented, with particular emphasis on the effect of the barrier in controlling the water and sediment content of the escaping mass. This aspect is achieved by implementing a new mechanical model into an enhanced two-phase dynamical mass flow model (Pudasaini, 2012), in which solid particles mixture and viscous fluid are taken into account. The complex mechanical model is defined as a function of the energy lost during impact, the physical and geometrical properties of the debris barrier, separate but strongly interacting dynamics of boulder and fluid flows during the impact, particle concentration distribution, and the slope characteristics. The particle-filtering-process results in a large variation in the rheological properties of the fluid-dominated escaping mass, including the substantial reduction in the bulk density, and the inertial forces of the debris-flows. Consequently, the destructive power and run
Perez-Rea, Daysi; Bergenståhl, Björn; Nilsson, Lars
2016-02-01
In this paper, we investigate whether dissolution in water under autoclaving conditions (140 °C, 20 min) or in dimethyl sulfoxide, DMSO (100 °C, 1 h), is preferable for characterization of amylose. Two types of amylose, potato and maize, were dissolved either in water using an autoclave or in DMSO. On the aqueous solutions obtained, the extent of molecular dissolution of the sample (referred to as the dissolution yield) was determined by enzymatic analysis as well as the molecular properties, such as molar mass and root-mean-square radius, obtained with asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and differential refractive index detection (AF4-MALS-dRI). The results showed that both dissolution methods are efficient at dissolving amylose. However, AF4-MALS-dRI analysis revealed substantial differences. Amylose aqueous solutions obtained by dissolution in DMSO were relatively stable over time, but the dissolution method in autoclave caused some degradation of the molecules, and their solutions display a high tendency to retrograde. PMID:26232931
DNS of Flows over Periodic Hills using a Discontinuous-Galerkin Spectral-Element Method
NASA Technical Reports Server (NTRS)
Diosady, Laslo T.; Murman, Scott M.
2014-01-01
Direct numerical simulation (DNS) of turbulent compressible flows is performed using a higher-order space-time discontinuous-Galerkin finite-element method. The numerical scheme is validated by performing DNS of the evolution of the Taylor-Green vortex and turbulent flow in a channel. The higher-order method is shown to provide increased accuracy relative to low-order methods at a given number of degrees of freedom. The turbulent flow over a periodic array of hills in a channel is simulated at Reynolds number 10,595 using an 8th-order scheme in space and a 4th-order scheme in time. These results are validated against previous large eddy simulation (LES) results. A preliminary analysis provides insight into how these detailed simulations can be used to improve Reynoldsaveraged Navier-Stokes (RANS) modeling
An explicit Runge-Kutta method for turbulent reacting flow calculations
NASA Technical Reports Server (NTRS)
Boretti, A. A.
1989-01-01
The paper presents a numerical method for the solution of the conservation equations governing steady, reacting, turbulent viscous flow in two-dimensional geometries, in both Cartesian and axisymmetric coordinates. These equations are written in Favre-averaged form and closed with a first order model. A two-equation K-epsilon model, where low Reynolds number and compressibility effects are included, and a modified eddy-break up model are used to simulate fluid mechanics turbulence, chemistry and turbulence-combustion interaction. The solution is obtained by using a pseudo-unsteady method with improved perturbation propagation properties. The equations are discretized in space by using a finite volume formulation. An explicit multi-stage dissipative Runge-Kutta algorithm is then used to advance the flow equations in the pseudo-time. The method is applied to the computation of both diffusion and premixed turbulent reacting flows. The computed temperature distributions compare favorably with experimental data.
Finite element approximations for quasi-Newtonian flows employing a multi-field GLS method
NASA Astrophysics Data System (ADS)
Zinani, Flávia; Frey, Sérgio
2011-08-01
This article concerns stabilized finite element approximations for flow-type sensitive fluid flows. A quasi-Newtonian model, based on a kinematic parameter of flow classification and shear and extensional viscosities, is used to represent the fluid behavior from pure shear up to pure extension. The flow governing equations are approximated by a multi-field Galerkin least-squares (GLS) method, in terms of strain rate, pressure and velocity ( D- p- u). This method, which may be viewed as an extension of the formulation for constant viscosity fluids introduced by Behr et al. (Comput Methods Appl Mech 104:31-48, 1993), allows the use of combinations of simple Lagrangian finite element interpolations. Mild Weissenberg flows of quasi-Newtonian fluids—using Carreau viscosities with power-law indexes varying from 0.2 to 2.5—are carried out through a four-to-one planar contraction. The performed physical analysis reveals that the GLS method provides a suitable approximation for the problem and the results are in accordance with the related literature.
Quasi-simultaneous interaction method for solving 2D boundary layer flows over plates and airfoils
NASA Astrophysics Data System (ADS)
Bijleveld, H. A.; Veldman, A. E. P.
2012-11-01
This paper studies unsteady 2D boundary layer flows over dented plates and a NACA 0012 airfoil. An inviscid flow is assumed to exist outside the boundary layer and is solved iteratively with the boundary layer flow together with the interaction method until a matching solution is achieved. Hereto a quasi-simultaneous interaction method is applied, in which the integral boundary layer equations are solved together with an interaction-law equation. The interaction-law equation is an approximation of the external flow and based on thin-airfoil theory. It is an algebraic relation between the velocity and displacement thickness. The interaction-law equation ensures that the eigenvalues of the system of equations do not have a sign change and that no singularities occur. Three numerical schemes are used to solve the boundary layer flow with the interaction method. These are: a standard scheme, a splitting method and a characteristics solver. All schemes use a finite difference discretization. The three schemes yield comparable results for the simulations carried out. The standard scheme is deviating most from the splitting and characteristics solvers. The results show that the eigenvalues remain positive, even in separation. As expected, the addition of the interaction-law equation prevents a sign change of the eigenvalues. The quasi-simultaneous interaction scheme is applicable to the three numerical schemes tested.
The Development of Loss of Flow Analysis Method for OPR1000 Using RETRAN
Dong Hyuk Lee; Yo-Han Kim; Chang-Kyung Sung
2006-07-01
A new loss of flow transient analysis method for OPR1000 (Optimized Power Reactor 1000, previously called KSNP: Korean Standard Nuclear Power Plant) based on RETRAN code were developed. The reference plant for the analysis is Ulchin Unit 3 and the transient analyzed is 4 pump coast-down. The current analysis for loss of RCS flow transient of OPR1000 uses COAST and CESEC codes. The new method uses RETRAN code to replace COAST and CESEC codes. Since the ability of RETRAN to replace CESEC has been studied in other non-LOCA transients, this paper will focus on COAST code and RCP coast-down flow rates. The results from simplified RETRAN nodalization corresponding to COAST show good agreement with RCS flow results from COAST code. The results are also compared with RETRAN base-deck for safety analysis which is more complex and show similar trends. Therefore, previous analysis method for loss of flow of OPR1000 using COAST code can be replaced with the new analysis method based on RETRAN. (authors)
NASA Technical Reports Server (NTRS)
Coakley, T. J.; Hsieh, T.
1985-01-01
Numerical simulation of steady and unsteady transonic diffuser flows using two different computer codes are discussed and compared with experimental data. The codes solve the Reynolds-averaged, compressible, Navier-Stokes equations using various turbulence models. One of the codes has been applied extensively to diffuser flows and uses the hybrid method of MacCormack. This code is relatively inefficient numerically. The second code, which was developed more recently, is fully implicit and is relatively efficient numerically. Simulations of steady flows using the implicit code are shown to be in good agreement with simulations using the hybrid code. Both simulations are in good agreement with experimental results. Simulations of unsteady flows using the two codes are in good qualitative agreement with each other, although the quantitative agreement is not as good as in the steady flow cases. The implicit code is shown to be eight times faster than the hybrid code for unsteady flow calculations and up to 32 times faster for steady flow calculations. Results of calculations using alternative turbulence models are also discussed.
NASA Technical Reports Server (NTRS)
Leballeur, J. C.; Girodroux-Lavigne, P.
1992-01-01
Three previous papers have shown that the viscous inviscid numerical methods were capable of calculating separated turbulent flows. The 'Semi-inverse method' and the models of the first author allow the computation of massive separations, stalled flows, and shock wave-boundary layer interactions, in two-dimensional or quasi-three-dimensional conditions, from low speeds to supersonic speeds. The 'Semi-implicit' method for time-consistent coupling allows for the computation of time-accurate transonic separated flow and buffer computations in two-dimensions. The present paper shows that the viscous-inviscid interaction approach is also able to compute the fully three-dimensional flow separation phenomena. The method is based on a thin-layer approximation of the theory of 'Defect-Formulation' that provides the viscous-inviscid splitting of the Navier-Stokes equations. A parametric analytical modelling of the 3D-turbulent velocity profiles is involved. Numerically, the 3D-velocity profiles are discretized in the normal z-direction and driven by parametric integral 3D-equations in direct or inverse modes in the x-direction. The viscous-inviscid coupling is fully 3D and solved the time-consistency problem with an extension of the 'Semi-implicit' method previously suggested in two-dimensions. A 3D inviscid subroutine with TSP approximation is used. Results are obtained for transonic steady flows over wings with shock-induced transonic separation. The method provides results for highly three-dimensional flow separations, such as induced by a three-dimensional through at the wall of a flat plate. The 3D viscous-inviscid coupling and the 3D model of the velocity field provide three-dimensional instantaneous skin-friction lines whose patterns exhibit the same complex topology as Navier-Stokes solvers, with foci, nodes, and saddle-points.
Stochastic finite difference lattice Boltzmann method for steady incompressible viscous flows
Fu, S.C.; So, R.M.C.; Leung, W.W.F.
2010-08-20
With the advent of state-of-the-art computers and their rapid availability, the time is ripe for the development of efficient uncertainty quantification (UQ) methods to reduce the complexity of numerical models used to simulate complicated systems with incomplete knowledge and data. The spectral stochastic finite element method (SSFEM) which is one of the widely used UQ methods, regards uncertainty as generating a new dimension and the solution as dependent on this dimension. A convergent expansion along the new dimension is then sought in terms of the polynomial chaos system, and the coefficients in this representation are determined through a Galerkin approach. This approach provides an accurate representation even when only a small number of terms are used in the spectral expansion; consequently, saving in computational resource can be realized compared to the Monte Carlo (MC) scheme. Recent development of a finite difference lattice Boltzmann method (FDLBM) that provides a convenient algorithm for setting the boundary condition allows the flow of Newtonian and non-Newtonian fluids, with and without external body forces to be simulated with ease. Also, the inherent compressibility effect in the conventional lattice Boltzmann method, which might produce significant errors in some incompressible flow simulations, is eliminated. As such, the FDLBM together with an efficient UQ method can be used to treat incompressible flows with built in uncertainty, such as blood flow in stenosed arteries. The objective of this paper is to develop a stochastic numerical solver for steady incompressible viscous flows by combining the FDLBM with a SSFEM. Validation against MC solutions of channel/Couette, driven cavity, and sudden expansion flows are carried out.
NASA Astrophysics Data System (ADS)
Yang, Hongli; Wu, Xinyuan; Fang, Yonglei; You, Xiong
2008-09-01
In this paper, extended Runge-Kutta-Nyström type methods for perturbed oscillators with low frequency are presented, which inherit the framework of RKN method and make full use of the special feature of the true flows. Following the line of J.Butcher, E.Hairer and G.Wanner, we develop a new kind of trees to derive the order conditions for the new methods.
NASA Astrophysics Data System (ADS)
Hilpert, Markus
2011-09-01
In this paper we demonstrate how the single and multiple-relaxation-time (MRT) lattice-Boltzmann (LB) method can be used to simulate hydrogeological flows under standard conditions. We explain in detail how real-world hydrogeological flow problems, which are formulated in terms of dimensional boundary and initial conditions, can be related to the nondimensional LB world, in which both the lattice spacing and the time step are nondimensional and typically set to unity. We first demonstrate the method in two examples where analytical solutions are known: steady state and transient flow of water in a square duct where fluid inertia is either negligible or not, respectively. Finally we simulate the flow of water in a sand where inertial forces are not negligible. For steady state flow we also present equations for calculating the permeability. Moreover, we demonstrate and advocate the usage of Richardson's extrapolation, which allows one to estimate LB modeling results for an infinite numerical resolution that include uncertainty intervals. For pressure-driven simulations of steady state creeping flow, we show how one can improve the numerical convergence behavior by performing "light-water" simulations at a lower Reynolds number while still predicting the correct velocity field.
NASA Astrophysics Data System (ADS)
Fosas de Pando, Miguel; Schmid, Peter J.; Sipp, Denis
2016-11-01
Nonlinear model reduction for large-scale flows is an essential component in many fluid applications such as flow control, optimization, parameter space exploration and statistical analysis. In this article, we generalize the POD-DEIM method, introduced by Chaturantabut & Sorensen [1], to address nonlocal nonlinearities in the equations without loss of performance or efficiency. The nonlinear terms are represented by nested DEIM-approximations using multiple expansion bases based on the Proper Orthogonal Decomposition. These extensions are imperative, for example, for applications of the POD-DEIM method to large-scale compressible flows. The efficient implementation of the presented model-reduction technique follows our earlier work [2] on linearized and adjoint analyses and takes advantage of the modular structure of our compressible flow solver. The efficacy of the nonlinear model-reduction technique is demonstrated to the flow around an airfoil and its acoustic footprint. We could obtain an accurate and robust low-dimensional model that captures the main features of the full flow.
Implicit high-order method for calculating rarefied gas flow in a planar microchannel
Titarev, V.A.
2012-01-01
An efficient numerical algorithm for calculating rarefied gas flows in planar microchannels on the basis of the Boltzmann kinetic equation with the linearized S-model collision integral is presented. The algorithm consists of a high-order spatial discretisation on unstructured meshes, conservative procedure to calculate macroscopic quantities and efficient one-step implicit time evolution method. It therefore works across all flow regimes from the free-molecular to nearly continuum ones and provides rapid convergence to steady state solutions. The parallel implementation is provided via MPI programming paradigm. The performance of the method is illustrated by computing the flow for length to the width ratios for up to 10{sup 3} in the wide range of Knudsen numbers. Numerical estimates of efficiency of implicit time marching and parallel performance as well as convergence studies in both physical and velocity spaces are provided. The end effects and other essentially two-dimensional features of the flow are analysed and a detailed comparison with the case of an infinitely long channel is carried out. The presented numerical data can be used as reference for future studies of nonlinear flows as well as for comparison with other numerical approaches and flow models.
NASA Astrophysics Data System (ADS)
Dixon, J. Brandon; Cote, Gerard; Gashev, Anatoly; Greiner, Steven; Moore, James; Zawieja, David
2006-02-01
Collecting microlymphatics play a vital role in promoting lymph flow from the initial lymphatics in the interstitial spaces to the large transport lymph ducts. In most tissues, the primary mechanism for producing this flow is the spontaneous contractions of the lymphatic wall. Individual units, known as lymphangion, are separated by valves that help prevent backflow when the vessel contracts, thus promoting flow through the lymphatic network. Lymphatic contractile activity is inhibited by flow in isolated lymphatics, however there are virtually no in situ measurements of lymph flow in these vessels. One of the difficulties associated with obtaining such measurements is the time consuming methods of manual particle tracking used previously by our group. Using an in situ preparation with mesenteric microlymphatics (~ 100 μm in diameter) and a high speed imaging system (500 fps), we have developed an image correlation method to measure lymphatic flow with a standard error of prediction of 0.3 mm/sec when compared with manual particle tracking.
Methods for numerical study of tube bundle vibrations in cross-flows
NASA Astrophysics Data System (ADS)
Longatte, E.; Bendjeddou, Z.; Souli, M.
2003-11-01
In many industrial applications, mechanical structures like heat exchanger tube bundles are subjected to complex flows causing possible vibrations and damage. Part of fluid forces are coupled with tube motion and the so-called fluid-elastic forces can affect the structure dynamic behaviour generating possible instabilities and leading to possible short term failures through high amplitude vibrations. Most classical fluid force identification methods rely on structure response experimental measurements associated with convenient data processes. Owing to recent improvements in Computational Fluid Dynamics, numerical simulation of flow-induced vibrations is now practicable for industrial purposes. The present paper is devoted to the numerical identification of fluid-elastic effects affecting tube bundle motion in presence of fluid at rest and one-phase cross-flows. What is the numerical process? When fluid-elastic effects are not significant and are restricted to added mass effects, there is no strong coupling between structure and fluid motions. The structure displacement is not supposed to affect flow patterns. Thus it is possible to solve flow and structure problems separately by using a fixed nonmoving mesh for the fluid dynamic computation. Power spectral density and time record of lift and drag forces acting on tube bundles can be computed numerically by using an unsteady fluid computation involving for example a large Eddy simulation. Fluid force spectra or time record can then be introduced as inlet conditions into the structure code providing the tube dynamic response generated by flow. Such a computation is not possible in presence of strong flow structure coupling. When fluid-elastic effects cannot be neglected, in presence of tube bundles subjected to cross-flows for example, a coupling between flow and structure computations is required. Appropriate numerical methods are investigated in the present work. The purpose is to be able to provide a numerical
NASA Technical Reports Server (NTRS)
Succi, G.
1982-01-01
The acoustical properties of locally and nonlocally reacting acoustical liners in grazing flow are described. The effect of mean flow and shear flow are considered as well as the application to rigid and limp bulk reacting materials. The axial wavenumber of the least attenuated mode in a flow duct is measured. The acoustical properties of duct liners is then deduced from the measured axial wavenumber and known flow profile and boundary conditions. This method is a natural extension of impedance-like measurements.
NASA Astrophysics Data System (ADS)
Hatanaka, K.; Hayashi, M.; Kawahara, M.
A novel FEM scheme that is based on the fractional-step method for solving time-dependent, incompressible viscous flow is presented and employed in the solution of a free-surface flow. The equations are given in indicial notation, as well as in the summation convention for repeated indices. The numerical results obtained exhibit good stability without the specification of Neumann conditions, and are compared in tabular form with the solutions given by Laitone's (1960) approximation. The method is also applicable to problems with artificial, open boundaries.
Fracture flow simulation using a finite-difference lattice Boltzmann method.
Kim, I; Lindquist, W B; Durham, W B
2003-04-01
We present numerical computations for single phase flow through three-dimensional digitized rock fractures under varied simulated confining pressures appropriate to midcrustal depths. The computations are performed using a finite difference, lattice Boltzmann method and thus simulate Navier-Stokes flow. The digitized fracture data sets come from profiled elevations taken on tensile induced fractures in Harcourt granite. Numerical predictions of fracture permeability are compared with laboratory measurements performed on the same fractures. Use of the finite difference lattice Boltzmann method allows computation on nonuniform grid spacing, enabling accurate resolution across the aperture width without extensive refinement in the other two directions.
Determination of heat flows under dynamic conditions using the method of parametric identification
NASA Astrophysics Data System (ADS)
But, E. N.; Simbirskii, D. F.
1982-10-01
An experimental-computational procedure is examined which allows the determination of the heat flows of a one-dimensional heat absorber from the results of the direct measurements of one or several local temperatures on the absorber. It is shown that in all cases, the proposed method involves solving an incorrectly formulated inverse heat conduction problem and can produce unstable or unreliable results. It is proposed to use parametric identification of the constant coefficients of a spline approximation of the unknown heat flows. The method is demonstrated for two real cases, with the temperature dependence of the thermophysical properties of the material taken into account in one of these cases.
Method of measuring the mass flow rate of a substance entering a cocurrent fluid stream
Cochran, Jr., Henry D.
1978-04-11
This invention relates to an improved method of monitoring the mass flow rate of a substance entering a cocurrent fluid stream. The method very basically consists of heating equal sections of the fluid stream above and below the point of entry of the substance to be monitored, and measuring and comparing the resulting change in temperature of the sections. Advantage is taken of the difference in thermal characteristics of the fluid and the substance to be measured to correlate temperature differences in the sections above and below the substance feed point for providing an indication of the mass flow rate of the substance.
Evidence flow graph methods for validation and verification of expert systems
NASA Technical Reports Server (NTRS)
Becker, Lee A.; Green, Peter G.; Bhatnagar, Jayant
1988-01-01
This final report describes the results of an investigation into the use of evidence flow graph techniques for performing validation and verification of expert systems. This was approached by developing a translator to convert horn-clause rule bases into evidence flow graphs, a simulation program, and methods of analysis. These tools were then applied to a simple rule base which contained errors. It was found that the method was capable of identifying a variety of problems, for example that the order of presentation of input data or small changes in critical parameters could effect the output from a set of rules.
Evidence flow graph methods for validation and verification of expert systems
NASA Technical Reports Server (NTRS)
Becker, Lee A.; Green, Peter G.; Bhatnagar, Jayant
1989-01-01
The results of an investigation into the use of evidence flow graph techniques for performing validation and verification of expert systems are given. A translator to convert horn-clause rule bases into evidence flow graphs, a simulation program, and methods of analysis were developed. These tools were then applied to a simple rule base which contained errors. It was found that the method was capable of identifying a variety of problems, for example that the order of presentation of input data or small changes in critical parameters could affect the output from a set of rules.
Simulation of Ferrofluid Flow for Magnetic Drug Targeting Using the Lattice Boltzmann Method
NASA Astrophysics Data System (ADS)
Kandelousi, Mohsen Sheikholeslami; Ellahi, Rahmat
2015-02-01
Influence of a spatially varying magnetic field on Fe3O4-plasma nanofluid flow in a vessel as a targeted drug delivery system is investigated. Combined effects of ferrohydrodynamic (FHD) and magnetohydrodynamic (MHD) are considered in mathematic models. The lattice Boltzmann method is applied to solve the governing equations. Effects of active parameters, such as the Reynolds number and magnetic number on the flow characteristics, have been examined. Results indicate that the presence of the magnetic field affects considerably the flow field. Back flow occurs near the region where the magnetic source is located. Also, it can be found that the skin friction coefficient is a decreasing function of the Reynolds number and magnetic number.
Multivariate data analysis methods for the interpretation of microbial flow cytometric data.
Davey, Hazel M; Davey, Christopher L
2011-01-01
Flow cytometry is an important technique in cell biology and immunology and has been applied by many groups to the analysis of microorganisms. This has been made possible by developments in hardware that is now sensitive enough to be used routinely for analysis of microbes. However, in contrast to advances in the technology that underpin flow cytometry, there has not been concomitant progress in the software tools required to analyse, display and disseminate the data and manual analysis, of individual samples remains a limiting aspect of the technology. We present two new data sets that illustrate common applications of flow cytometry in microbiology and demonstrate the application of manual data analysis, automated visualisation (including the first description of a new piece of software we are developing to facilitate this), genetic programming, principal components analysis and artificial neural nets to these data. The data analysis methods described here are equally applicable to flow cytometric applications with other cell types.
A hot-wire method for high-intensity turbulent flows
NASA Technical Reports Server (NTRS)
Mueller, U. R.
1983-01-01
A measuring technique for determing instantaneous, three-dimensional velocity vectors in highly turbulent flows by means of a 4-sensor hot-wire probe is described. As is well known, the hot-wire signal received in reversing flows cannot uniquely be interpreted. This difficulty is circumvented by tracking the thermal wake of a heated wire. Whenever the approximate flow direction is indicated by a temperature-sensitive wake detector, all components of the instantaneous velocity vector are evaluated by means of a digital data reduction method. Uniqueness of the solution derived from the triple-hot-wire response equations is examined. A first application of the proposed measuring technique in the recirculating flow downstream of a backward-facing step is described.
Time-marching methods for three-dimensional steady and unsteady viscous imcompressible flows
NASA Technical Reports Server (NTRS)
Hsu, C.-H.; Chen, Y.-M.; Liu, C. H.
1991-01-01
An implicit algorithm for the solution of three-dimensional, steady and unsteady, viscous, incompressible flows is presented. The algorithm is based on an upwind-relaxation finite-difference method. Steady-state solutions are carried out using a time-marching solution technique in combination with a local time-stepping strategy. To obtain time-accurate solutions, a subiterative procedure is employed at each physical time step using a global time step to ensure the divergence-free condition. Steady-state flows in several straight ducts and in a square duct with a 90-degree bend are computed and compared with analytical and experimental results. The classical problem of starting flow in a circular pipe is chosen to verify the time accuracy of the present scheme. Finally, the three-dimensional bubble-type vortex breakdown of a slender cylindrical vortex in an unbounded flow is investigated.
NASA Technical Reports Server (NTRS)
Walitt, L.; Trulio, J. G.
1971-01-01
A numerical method is presented for the calculation of steady, three-dimensional, viscous, compressible flow fields about slender bodies at angle of attack and at supersonic speeds. Approximations are introduced in modeling the flow in the longitudinal direction. Accordingly, the flow fields calculated with the program were computed with a model that permits viscous crossflow together with inviscid axial flow. An analysis of the errors introduced by such a treatment is presented. Numerical calculations were made and compared with experimental results for an ogive-cylinder and an airplane fuselage configuration. Generally, good agreement with experiment was obtained. However, boundary layer separation and body vortex positions differed from experimental locations on the ogive-cylinder, and the shock induced by the fuselage canopy was predicted at a slightly different location.
A two-dimensional adaptive spectral element method for the direct simulation of incompressible flow
NASA Astrophysics Data System (ADS)
Hsu, Li-Chieh
The spectral element method is a high order discretization scheme for the solution of nonlinear partial differential equations. The method draws its strengths from the finite element method for geometrical flexibility and spectral methods for high accuracy. Although the method is, in theory, very powerful for complex phenomena such as transitional flows, its practical implementation is limited by the arbitrary choice of domain discretization. For instance, it is hard to estimate the appropriate number of elements for a specific case. Selection of regions to be refined or coarsened is difficult especially as the flow becomes more complex and memory limits of the computer are stressed. We present an adaptive spectral element method in which the grid is automatically refined or coarsened in order to capture underresolved regions of the domain and to follow regions requiring high resolution as they develop in time. The objective is to provide the best and most efficient solution to a time-dependent nonlinear problem by continually optimizing resource allocation. The adaptivity is based on an error estimator which determines which regions need more resolution. The solution strategy is as follows: compute an initial solution with a suitable initial mesh, estimate errors in the solution locally in each element, modify the mesh according to the error estimators, interpolate old mesh solutions onto the new elements, and resume the numerical solution process. A two-dimensional adaptive spectral element method for the direct simulation of incompressible flows has been developed. The adaptive algorithm effectively diagnoses and refines regions of the flow where complexity of the solution requires increased resolution. The method has been demonstrated on two-dimensional examples in heat conduction, Stokes and Navier-Stokes flows.
The design and fabrication of two portal vein flow phantoms by different methods
Yunker, Bryan E. Lanning, Craig J.; Shandas, Robin; Hunter, Kendall S.; Chen, S. James
2014-02-15
Purpose: This study outlines the design and fabrication techniques for two portal vein flow phantoms. Methods: A materials study was performed as a precursor to this phantom fabrication effort and the desired material properties are restated for continuity. A three-dimensional portal vein pattern was created from the Visual Human database. The portal vein pattern was used to fabricate two flow phantoms by different methods with identical interior surface geometry using computer aided design software tools and rapid prototyping techniques. One portal flow phantom was fabricated within a solid block of clear silicone for use on a table with Ultrasound or within medical imaging systems such as MRI, CT, PET, or SPECT. The other portal flow phantom was fabricated as a thin walled tubular latex structure for use in water tanks with Ultrasound imaging. Both phantoms were evaluated for usability and durability. Results: Both phantoms were fabricated successfully and passed durability criteria for flow testing in the next project phase. Conclusions: The fabrication methods and materials employed for the study yielded durable portal vein phantoms.
NASA Astrophysics Data System (ADS)
Witzke, V.; Silvers, L. J.; Favier, B.
2016-08-01
Shear flows are ubiquitous in astrophysical objects including planetary and stellar interiors, where their dynamics can have significant impact on thermo-chemical processes. Investigating the complex dynamics of shear flows requires numerical calculations that provide a long time evolution of the system. To achieve a sufficiently long lifetime in a local numerical model the system has to be forced externally. However, at present, there exist several different forcing methods to sustain large-scale shear flows in local models. In this paper we examine and compare various methods used in the literature in order to resolve their respective applicability and limitations. These techniques are compared during the exponential growth phase of a shear flow instability, such as the Kelvin-Helmholtz (KH) instability, and some are examined during the subsequent non-linear evolution. A linear stability analysis provides reference for the growth rate of the most unstable modes in the system and a detailed analysis of the energetics provides a comprehensive understanding of the energy exchange during the system's evolution. Finally, we discuss the pros and cons of each forcing method and their relation with natural mechanisms generating shear flows.
Pereira, Luciano; Bittencourt, Paulo R L; Oliveira, Rafael S; Junior, Mauro B M; Barros, Fernanda V; Ribeiro, Rafael V; Mazzafera, Paulo
2016-07-01
Wood contains a large amount of air, even in functional xylem. Air embolisms in the xylem affect water transport and can determine plant growth and survival. Embolisms are usually estimated with laborious hydraulic methods, which can be prone to several artefacts. Here, we describe a new method for estimating embolisms that is based on air flow measurements of entire branches. To calculate the amount of air flowing out of the branch, a vacuum was applied to the cut bases of branches under different water potentials. We first investigated the source of air by determining whether it came from inside or outside the branch. Second, we compared embolism curves according to air flow or hydraulic measurements in 15 vessel- and tracheid-bearing species to test the hypothesis that the air flow is related to embolism. Air flow came almost exclusively from air inside the branch during the 2.5-min measurements and was strongly related to embolism. We propose a new embolism measurement method that is simple, effective, rapid and inexpensive, and that allows several measurements on the same branch, thus opening up new possibilities for studying plant hydraulics.
NASA Astrophysics Data System (ADS)
Witzke, V.; Silvers, L. J.; Favier, B.
2016-11-01
Shear flows are ubiquitous in astrophysical objects including planetary and stellar interiors, where their dynamics can have significant impact on thermochemical processes. Investigating the complex dynamics of shear flows requires numerical calculations that provide a long-time evolution of the system. To achieve a sufficiently long lifetime in a local numerical model, the system has to be forced externally. However, at present, there exist several different forcing methods to sustain large-scale shear flows in local models. In this paper, we examine and compare various methods used in the literature in order to resolve their respective applicability and limitations. These techniques are compared during the exponential growth phase of a shear flow instability, such as the Kelvin-Helmholtz (KH) instability, and some are examined during the subsequent non-linear evolution. A linear stability analysis provides reference for the growth rate of the most unstable modes in the system and a detailed analysis of the energetics provides a comprehensive understanding of the energy exchange during the system's evolution. Finally, we discuss the pros and cons of each forcing method and their relation with natural mechanisms generating shear flows.
Pereira, Luciano; Bittencourt, Paulo R L; Oliveira, Rafael S; Junior, Mauro B M; Barros, Fernanda V; Ribeiro, Rafael V; Mazzafera, Paulo
2016-07-01
Wood contains a large amount of air, even in functional xylem. Air embolisms in the xylem affect water transport and can determine plant growth and survival. Embolisms are usually estimated with laborious hydraulic methods, which can be prone to several artefacts. Here, we describe a new method for estimating embolisms that is based on air flow measurements of entire branches. To calculate the amount of air flowing out of the branch, a vacuum was applied to the cut bases of branches under different water potentials. We first investigated the source of air by determining whether it came from inside or outside the branch. Second, we compared embolism curves according to air flow or hydraulic measurements in 15 vessel- and tracheid-bearing species to test the hypothesis that the air flow is related to embolism. Air flow came almost exclusively from air inside the branch during the 2.5-min measurements and was strongly related to embolism. We propose a new embolism measurement method that is simple, effective, rapid and inexpensive, and that allows several measurements on the same branch, thus opening up new possibilities for studying plant hydraulics. PMID:26918522
Gu, Ben J; Sun, Chun; Fuller, Stephen; Skarratt, Kristen K; Petrou, Steven; Wiley, James S
2014-04-01
Phagocytosis is central to immunity however a rapid and standardized method is much needed for quantitative assessment of the phagocytic process. We describe a real-time flow cytometric method to quantitate the phagocytosis of fluorescent latex beads by human monocytes in serum-free conditions. Effects of buffer composition, temperature, pH, and bead surface on phagocytic rate are described. The innate phagocytic ability of human monocytes from single subjects measured by this method was relatively stable over many months although phagocytosis rate varied as much as two-fold between individuals. Comparable results were obtained with a simplified method using several mL of whole blood which is suitable for routine clinical application. This method also allows two-color flow cytometric measurement of cytosolic calcium levels during the phagocytic uptake of fluorescent beads.
NASA Technical Reports Server (NTRS)
Cook, C. H.
1977-01-01
The results of a comprehensive numerical investigation of the basic capabilities of the finite element method (FEM) for numerical solution of compressible flow problems governed by the two-dimensional and axis-symmetric Navier-Stokes equations in primitive variables are presented. The strong and weak points of the method as a tool for computational fluid dynamics are considered. The relation of the linear element finite element method to finite difference methods (FDM) is explored. The calculation of free shear layer and separated flows over aircraft boattail afterbodies with plume simulators indicate the strongest assets of the method are its capabilities for reliable and accurate calculation employing variable grids which readily approximate complex geometry and capably adapt to the presence of diverse regions of large solution gradients without the necessity of domain transformation.
Weak Galerkin finite element methods for Darcy flow: Anisotropy and heterogeneity
Lin, Guang; Liu, Jiangguo; Mu, Lin; Ye, Xiu
2014-10-11
This paper presents a family of weak Galerkin finite element methods (WGFEMs) for Darcy flow computation. The WGFEMs are new numerical methods that rely on the novel concept of discrete weak gradients. The WGFEMs solve for pressure unknowns both in element interiors and on the mesh skeleton. The numerical velocity is then obtained from the discrete weak gradient of the numerical pressure. The new methods are quite different than many existing numerical methods in that they are locally conservative by design, the resulting discrete linear systems are symmetric and positive-definite, and there is no need for tuning problem-dependent penalty factors. We test the WGFEMs on benchmark problems to demonstrate the strong potential of these new methods in handling strong anisotropy and heterogeneity in Darcy flow.
A coupled Eulerian/Lagrangian method for the solution of three-dimensional vortical flows
NASA Technical Reports Server (NTRS)
Felici, Helene Marie
1992-01-01
A coupled Eulerian/Lagrangian method is presented for the reduction of numerical diffusion observed in solutions of three-dimensional rotational flows using standard Eulerian finite-volume time-marching procedures. A Lagrangian particle tracking method using particle markers is added to the Eulerian time-marching procedure and provides a correction of the Eulerian solution. In turn, the Eulerian solutions is used to integrate the Lagrangian state-vector along the particles trajectories. The Lagrangian correction technique does not require any a-priori information on the structure or position of the vortical regions. While the Eulerian solution ensures the conservation of mass and sets the pressure field, the particle markers, used as 'accuracy boosters,' take advantage of the accurate convection description of the Lagrangian solution and enhance the vorticity and entropy capturing capabilities of standard Eulerian finite-volume methods. The combined solution procedures is tested in several applications. The convection of a Lamb vortex in a straight channel is used as an unsteady compressible flow preservation test case. The other test cases concern steady incompressible flow calculations and include the preservation of turbulent inlet velocity profile, the swirling flow in a pipe, and the constant stagnation pressure flow and secondary flow calculations in bends. The last application deals with the external flow past a wing with emphasis on the trailing vortex solution. The improvement due to the addition of the Lagrangian correction technique is measured by comparison with analytical solutions when available or with Eulerian solutions on finer grids. The use of the combined Eulerian/Lagrangian scheme results in substantially lower grid resolution requirements than the standard Eulerian scheme for a given solution accuracy.
NASA Astrophysics Data System (ADS)
Fan, Xiaofeng; Wang, Jiangfeng
2016-06-01
The atomization of liquid fuel is a kind of intricate dynamic process from continuous phase to discrete phase. Procedures of fuel spray in supersonic flow are modeled with an Eulerian-Lagrangian computational fluid dynamics methodology. The method combines two distinct techniques and develops an integrated numerical simulation method to simulate the atomization processes. The traditional finite volume method based on stationary (Eulerian) Cartesian grid is used to resolve the flow field, and multi-component Navier-Stokes equations are adopted in present work, with accounting for the mass exchange and heat transfer occupied by vaporization process. The marker-based moving (Lagrangian) grid is utilized to depict the behavior of atomized liquid sprays injected into a gaseous environment, and discrete droplet model 13 is adopted. To verify the current approach, the proposed method is applied to simulate processes of liquid atomization in supersonic cross flow. Three classic breakup models, TAB model, wave model and K-H/R-T hybrid model, are discussed. The numerical results are compared with multiple perspectives quantitatively, including spray penetration height and droplet size distribution. In addition, the complex flow field structures induced by the presence of liquid spray are illustrated and discussed. It is validated that the maker-based Eulerian-Lagrangian method is effective and reliable.
NASA Astrophysics Data System (ADS)
Rapaka, Narsimha R.; Sarkar, Sutanu
2016-10-01
A sharp-interface Immersed Boundary Method (IBM) is developed to simulate density-stratified turbulent flows in complex geometry using a Cartesian grid. The basic numerical scheme corresponds to a central second-order finite difference method, third-order Runge-Kutta integration in time for the advective terms and an alternating direction implicit (ADI) scheme for the viscous and diffusive terms. The solver developed here allows for both direct numerical simulation (DNS) and large eddy simulation (LES) approaches. Methods to enhance the mass conservation and numerical stability of the solver to simulate high Reynolds number flows are discussed. Convergence with second-order accuracy is demonstrated in flow past a cylinder. The solver is validated against past laboratory and numerical results in flow past a sphere, and in channel flow with and without stratification. Since topographically generated internal waves are believed to result in a substantial fraction of turbulent mixing in the ocean, we are motivated to examine oscillating tidal flow over a triangular obstacle to assess the ability of this computational model to represent nonlinear internal waves and turbulence. Results in laboratory-scale (order of few meters) simulations show that the wave energy flux, mean flow properties and turbulent kinetic energy agree well with our previous results obtained using a body-fitted grid (BFG). The deviation of IBM results from BFG results is found to increase with increasing nonlinearity in the wave field that is associated with either increasing steepness of the topography relative to the internal wave propagation angle or with the amplitude of the oscillatory forcing. LES is performed on a large scale ridge, of the order of few kilometers in length, that has the same geometrical shape and same non-dimensional values for the governing flow and environmental parameters as the laboratory-scale topography, but significantly larger Reynolds number. A non-linear drag law
Roach, P.D.; Raptis, A.C.
1980-11-24
A method and apparatus for monitoring char flow in a coal gasifier system includes flow monitor circuits which measure acoustic attenuation caused by the presence of char in a char line and provides a char flow/no flow indication and an indication of relative char density. The flow monitor circuits compute the ratio of signals in two frequency bands, a first frequency band representative of background noise, and a second higher frequency band in which background noise is attenuated by the presence of char. Since the second frequency band contains higher frequencies, the ratio can be used to provide a flow/no flow indication. The second band can also be selected so that attenuation is monotonically related to particle concentration, providing a quantitative measure of char concentration.
On Efficient Multigrid Methods for Materials Processing Flows with Small Particles
NASA Technical Reports Server (NTRS)
Thomas, James (Technical Monitor); Diskin, Boris; Harik, VasylMichael
2004-01-01
Multiscale modeling of materials requires simulations of multiple levels of structural hierarchy. The computational efficiency of numerical methods becomes a critical factor for simulating large physical systems with highly desperate length scales. Multigrid methods are known for their superior efficiency in representing/resolving different levels of physical details. The efficiency is achieved by employing interactively different discretizations on different scales (grids). To assist optimization of manufacturing conditions for materials processing with numerous particles (e.g., dispersion of particles, controlling flow viscosity and clusters), a new multigrid algorithm has been developed for a case of multiscale modeling of flows with small particles that have various length scales. The optimal efficiency of the algorithm is crucial for accurate predictions of the effect of processing conditions (e.g., pressure and velocity gradients) on the local flow fields that control the formation of various microstructures or clusters.
Spectral element-Fourier method for unsteady conjugate heat transfer in complex geometry flows
NASA Astrophysics Data System (ADS)
Amon, Cristina H.
1995-04-01
A spectral-element Fourier method (SEFM) is presented for the direct numerical simulation of forced convective heat transfer and conjugate conduction/convection in transitional internal flows in complex geometries. The SEFM is employed for the spatial discretization of the unsteady, incompressible, three-dimensional Navier-Stokes and energy equations. The resulting discrete equations are solved by a semi-implicit method in time treating explicitly the convection operator and implicitly the remaining pressure and viscous contributions. This methodology is illustrated by performing direct numerical simulations to investigate forced convective heat transfer in supercritical self-sustained oscillatory flows and conjugate effects in multimaterial domains. Highly unsteady flows in complex geometries are considered, including modified channels with periodic inhomogeneities such as spanwise rectangular and triangular grooves encountered in electronic equipment and compact heat exchangers.
The High-Resolution Wave-Propagation Method Applied to Meso- and Micro-Scale Flows
NASA Technical Reports Server (NTRS)
Ahmad, Nashat N.; Proctor, Fred H.
2012-01-01
The high-resolution wave-propagation method for computing the nonhydrostatic atmospheric flows on meso- and micro-scales is described. The design and implementation of the Riemann solver used for computing the Godunov fluxes is discussed in detail. The method uses a flux-based wave decomposition in which the flux differences are written directly as the linear combination of the right eigenvectors of the hyperbolic system. The two advantages of the technique are: 1) the need for an explicit definition of the Roe matrix is eliminated and, 2) the inclusion of source term due to gravity does not result in discretization errors. The resulting flow solver is conservative and able to resolve regions of large gradients without introducing dispersion errors. The methodology is validated against exact analytical solutions and benchmark cases for non-hydrostatic atmospheric flows.
Assessment of an Unstructured-Grid Method for Predicting 3-D Turbulent Viscous Flows
NASA Technical Reports Server (NTRS)
Frink, Neal T.
1996-01-01
A method Is presented for solving turbulent flow problems on three-dimensional unstructured grids. Spatial discretization Is accomplished by a cell-centered finite-volume formulation using an accurate lin- ear reconstruction scheme and upwind flux differencing. Time is advanced by an implicit backward- Euler time-stepping scheme. Flow turbulence effects are modeled by the Spalart-Allmaras one-equation model, which is coupled with a wall function to reduce the number of cells in the sublayer region of the boundary layer. A systematic assessment of the method is presented to devise guidelines for more strategic application of the technology to complex problems. The assessment includes the accuracy In predictions of skin-friction coefficient, law-of-the-wall behavior, and surface pressure for a flat-plate turbulent boundary layer, and for the ONERA M6 wing under a high Reynolds number, transonic, separated flow condition.
A method to calculate finite-time Lyapunov exponents for inertial particles in incompressible flows
NASA Astrophysics Data System (ADS)
Garaboa-Paz, D.; Pérez-Muñuzuri, V.
2015-10-01
The present study aims to improve the calculus of finite-time Lyapunov exponents (FTLEs) applied to describe the transport of inertial particles in a fluid flow. To this aim, the deformation tensor is modified to take into account that the stretching rate between particles separated by a certain distance is influenced by the initial velocity of the particles. Thus, the inertial FTLEs (iFTLEs) are defined in terms of the maximum stretching between infinitesimally close trajectories that have different initial velocities. The advantages of this improvement, if compared to the standard method (Shadden et al., 2005), are discussed for the double-gyre flow and the meandering jet flow. The new method allows one to identify the initial velocity that inertial particles must have in order to maximize their dispersion.
Morin, Roger H.; Olsen, Harold W.; Nelson, Karl R.; Gill, James D.
1989-01-01
A graphical method has been developed for determining the coefficient of consolidation from the transient phases of a flow-pump permeability test. The flow pump can be used to infuse fluid into or withdraw fluid from a laboratory sediment specimen at a constant volumetric rate in order to obtain data that can be used to calculate permeability using Darcy's law. Representative type-curve solutions to the associated forced-flow and pressure-decay models are derived. These curves provide the basis for graphically evaluating the permeability k, the coefficient of consolidation cv, and the coefficient of volume change mv. The curve-matching technique is easy and rapid. Values of k, cv and mv for a laterally confined kaolinite specimen were determined by this graphical method and appear to be in reasonably good agreement with numerically derived estimates (within 20%). Discrepancies between the two sets of results seem to be largely a function of data quality.
A solution of two-dimensional magnetohydrodynamic flow using the finite element method
Verardi, S.L.L.; Cardoso, J.R.; Motta, C.C.
1998-09-01
The problem of magnetohydrodynamic flow through channels has become important because of several engineering applications such as design of nuclear reactor cooling systems, electromagnetic pumps, MHD flowmeters, MHD generators, blood flow measurements, etc. A numerical code based on the Finite Element Method (FEM) was developed to solve the two-dimensional, steady-state magnetohydrodynamic (MHD) flow in a rectangular channel. In order to apply the FEM, the Galerkin Weak Formulation was used. In this analysis, in contrast with the previous works, the thickness of the duct wall is taken into account and the results are compared to those obtained in the limit case when the thickness is much smaller than a characteristic dimension of the duct. In this case, convergence behavior of several iterative methods, for high Hartmann numbers, was also investigated.
Assessment of an Unstructured-Grid Method for Predicting 3-D Turbulent Viscous Flows
NASA Technical Reports Server (NTRS)
Frink, Neal T.
1996-01-01
A method is presented for solving turbulent flow problems on three-dimensional unstructured grids. Spatial discretization is accomplished by a cell-centered finite-volume formulation using an accurate linear reconstruction scheme and upwind flux differencing. Time is advanced by an implicit backward-Euler time-stepping scheme. Flow turbulence effects are modeled by the Spalart-Allmaras one-equation model, which is coupled with a wall function to reduce the number of cells in the sublayer region of the boundary layer. A systematic assessment of the method is presented to devise guidelines for more strategic application of the technology to complex problems. The assessment includes the accuracy in predictions of skin-friction coefficient, law-of-the-wall behavior, and surface pressure for a flat-plate turbulent boundary layer, and for the ONERA M6 wing under a high Reynolds number, transonic, separated flow condition.
Pfeiffer, Valentin; Barbeau, Benoit
2014-02-01
Despite its shortcomings, the T10 method introduced by the United States Environmental Protection Agency (USEPA) in 1989 is currently the method most frequently used in North America to calculate disinfection performance. Other methods (e.g., the Integrated Disinfection Design Framework, IDDF) have been advanced as replacements, and more recently, the USEPA suggested the Extended T10 and Extended CSTR (Continuous Stirred-Tank Reactor) methods to improve the inactivation calculations within ozone contactors. To develop a method that fully considers the hydraulic behavior of the contactor, two models (Plug Flow with Dispersion and N-CSTR) were successfully fitted with five tracer tests results derived from four Water Treatment Plants and a pilot-scale contactor. A new method based on the N-CSTR model was defined as the Partially Segregated (Pseg) method. The predictions from all the methods mentioned were compared under conditions of poor and good hydraulic performance, low and high disinfectant decay, and different levels of inactivation. These methods were also compared with experimental results from a chlorine pilot-scale contactor used for Escherichia coli inactivation. The T10 and Extended T10 methods led to large over- and under-estimations. The Segregated Flow Analysis (used in the IDDF) also considerably overestimated the inactivation under high disinfectant decay. Only the Extended CSTR and Pseg methods produced realistic and conservative predictions in all cases. Finally, a simple implementation procedure of the Pseg method was suggested for calculation of disinfection performance.
NASA Astrophysics Data System (ADS)
Angelidis, Dionysios; Chawdhary, Saurabh; Sotiropoulos, Fotis
2016-11-01
A novel numerical method is developed for solving the 3D, unsteady, incompressible Navier-Stokes equations on locally refined fully unstructured Cartesian grids in domains with arbitrarily complex immersed boundaries. Owing to the utilization of the fractional step method on an unstructured Cartesian hybrid staggered/non-staggered grid layout, flux mismatch and pressure discontinuity issues are avoided and the divergence free constraint is inherently satisfied to machine zero. Auxiliary/hanging nodes are used to facilitate the discretization of the governing equations. The second-order accuracy of the solver is ensured by using multi-dimension Lagrange interpolation operators and appropriate differencing schemes at the interface of regions with different levels of refinement. The sharp interface immersed boundary method is augmented with local near-boundary refinement to handle arbitrarily complex boundaries. The discrete momentum equation is solved with the matrix free Newton-Krylov method and the Krylov-subspace method is employed to solve the Poisson equation. The second-order accuracy of the proposed method on unstructured Cartesian grids is demonstrated by solving the Poisson equation with a known analytical solution. A number of three-dimensional laminar flow simulations of increasing complexity illustrate the ability of the method to handle flows across a range of Reynolds numbers and flow regimes. Laminar steady and unsteady flows past a sphere and the oblique vortex shedding from a circular cylinder mounted between two end walls demonstrate the accuracy, the efficiency and the smooth transition of scales and coherent structures across refinement levels. Large-eddy simulation (LES) past a miniature wind turbine rotor, parameterized using the actuator line approach, indicates the ability of the fully unstructured solver to simulate complex turbulent flows. Finally, a geometry resolving LES of turbulent flow past a complete hydrokinetic turbine illustrates
NASA Astrophysics Data System (ADS)
Kratzsch, C.; Asad, A.; Schwarze, R.
2016-07-01
Modeling of the processes in the continuous casting mold engaged many scientists once the computer-technology was able to accomplish that task. Despite that, CFD modeling of the fluid flow is still challenging. The methods allow deeper and deeper inside views into transient flow processes. Mostly two kinds of methods are applied for this purpose. URANS simulations are used for a coarse overview of the transient behavior on scales determined by the big rollers inside the mold. Besides, LES were done to study the processes on smaller scales. Unfortunately, the effort to set up a LES is orders of magnitude higher in time and space compared to URANS. Often, the flow determining processes take place in small areas inside the flow domain. Hence, scale resolving methods (SRS) came up, which resolve the turbulence in some amount in these regions, whereas they go back to URANS in the regions of less importance. It becomes more complex when dealing with magnetic fields in terms of EMBr devices. The impact of electro magnetically forces changes the flow structure remarkably. Many important effects occur, e.g. MHD turbulence, which are attributable to processes on large turbulent scales. To understand the underlying phenomena in detail, SRS allows a good inside view by resolving these processes partially. This study compares two of these methods, namely the Scale Adaptive Simulation (SAS) and the Delayed Detached Eddy Simulation (DDES), with respect to rendition of the results known from experiments and URANS simulation. The results show, that the SAS as well as the DDES are able to deliver good results with higher mesh resolutions in important regions in the flow domain
NASA Astrophysics Data System (ADS)
Takahashi, Ryohei; Mamori, Hiroya; Yamamoto, Makoto
2016-02-01
A numerical method for simulating gas-liquid-solid three-phase flows based on the moving particle semi-implicit (MPS) approach was developed in this study. Computational instability often occurs in multiphase flow simulations if the deformations of the free surfaces between different phases are large, among other reasons. To avoid this instability, this paper proposes an improved coupling procedure between different phases in which the physical quantities of particles in different phases are calculated independently. We performed numerical tests on two illustrative problems: a dam-break problem and a solid-sphere impingement problem. The former problem is a gas-liquid two-phase problem, and the latter is a gas-liquid-solid three-phase problem. The computational results agree reasonably well with the experimental results. Thus, we confirmed that the proposed MPS method reproduces the interaction between different phases without inducing numerical instability.
Advanced numerical methods for three dimensional two-phase flow calculations
Toumi, I.; Caruge, D.
1997-07-01
This paper is devoted to new numerical methods developed for both one and three dimensional two-phase flow calculations. These methods are finite volume numerical methods and are based on the use of Approximate Riemann Solvers concepts to define convective fluxes versus mean cell quantities. The first part of the paper presents the numerical method for a one dimensional hyperbolic two-fluid model including differential terms as added mass and interface pressure. This numerical solution scheme makes use of the Riemann problem solution to define backward and forward differencing to approximate spatial derivatives. The construction of this approximate Riemann solver uses an extension of Roe`s method that has been successfully used to solve gas dynamic equations. As far as the two-fluid model is hyperbolic, this numerical method seems very efficient for the numerical solution of two-phase flow problems. The scheme was applied both to shock tube problems and to standard tests for two-fluid computer codes. The second part describes the numerical method in the three dimensional case. The authors discuss also some improvements performed to obtain a fully implicit solution method that provides fast running steady state calculations. Such a scheme is not implemented in a thermal-hydraulic computer code devoted to 3-D steady-state and transient computations. Some results obtained for Pressurised Water Reactors concerning upper plenum calculations and a steady state flow in the core with rod bow effect evaluation are presented. In practice these new numerical methods have proved to be stable on non staggered grids and capable of generating accurate non oscillating solutions for two-phase flow calculations.
Zheng, Lin; Guo, Zhaoli; Shi, Baochang
2012-07-01
The lattice Boltzmann equation (LBE) method has been shown to be a promising tool for microscale gas flows. However, few works focus on the microtube flows, and there still are some fundamental problems for the LBE to such flows. In this paper, a recently proposed axisymmetric LBE with three kinetic boundary conditions, i.e., the combination of bounceback and specular reflection scheme, the combination of the Maxwell and specular-reflection scheme, and the combination of the Maxwell and bounceback scheme, have been investigated in detail. By analyzing the micro-Hagen-Poiseuille flow, we observed the discrete boundary condition effect and provided a revised boundary scheme to overcome such effect near the slip flow regime. Some numerical tests for the micro-Hagen-Poiseuille have been carried out to validate the analysis, and the numerical results of the revised boundary schemes agree well with the analytic solutions which confirmed our theoretical analysis. In addition, we also applied the revised combination of the Maxwell and bounceback scheme to microtube flow with sudden expansion and contraction, the numerical results of the pressure distribution and normalized slip velocity agree well with the theoretical ones. PMID:23005568
Simulation of Two Phase Fluid Flow With Various Kinds of Barriers Using Lattice Boltzmann Method
NASA Astrophysics Data System (ADS)
Wijaya, Imam; Purqon, Acep
2016-08-01
Multiphase fluid flow in a pore medium is a problem that is very interesting to be learned. In its flow, the fluid can experience a few of barriers / obstacles like the exsisting of things in the flow medium. The existence of the barriers can detain the rate speed of the fluid flow. The barries that its form is different will provide influence to the speed of of fluid flow that is different as well. To know the influence of barriers form twards the profile of fluid speed rate, is conducted by the simulation by using Lattice Boltzmann Methode (LBM). In this simulation, the barriers is varied in the form of circle, square, and ellips. From simulation that is conducted, to known the influence of barriers variations twards the fluid speed, ploted by the graph of the fluid speed relations along simulation time and plotted by the fluid speed vector in each posisition. From the simulation, it is obtained that the barriers with square formed produced the higest speed rate of the fluid flow, with the speed rate 0.26 lu/ts, then circle formed with the speed rate 0.24 lu/ts, and the last square formed with speed rate 0.24 lu/ts.
Applications of Taylor-Galerkin finite element method to compressible internal flow problems
NASA Technical Reports Server (NTRS)
Sohn, Jeong L.; Kim, Yongmo; Chung, T. J.
1989-01-01
A two-step Taylor-Galerkin finite element method with Lapidus' artificial viscosity scheme is applied to several test cases for internal compressible inviscid flow problems. Investigations for the effect of supersonic/subsonic inlet and outlet boundary conditions on computational results are particularly emphasized.
Mittal, R.; Dong, H.; Bozkurttas, M.; Najjar, F.M.; Vargas, A.; von Loebbecke, A.
2010-01-01
A sharp interface immersed boundary method for simulating incompressible viscous flow past three-dimensional immersed bodies is described. The method employs a multi-dimensional ghost-cell methodology to satisfy the boundary conditions on the immersed boundary and the method is designed to handle highly complex three-dimensional, stationary, moving and/or deforming bodies. The complex immersed surfaces are represented by grids consisting of unstructured triangular elements; while the flow is computed on non-uniform Cartesian grids. The paper describes the salient features of the methodology with special emphasis on the immersed boundary treatment for stationary and moving boundaries. Simulations of a number of canonical two- and three-dimensional flows are used to verify the accuracy and fidelity of the solver over a range of Reynolds numbers. Flow past suddenly accelerated bodies are used to validate the solver for moving boundary problems. Finally two cases inspired from biology with highly complex three-dimensional bodies are simulated in order to demonstrate the versatility of the method. PMID:20216919
Profile Optimization Method for Robust Airfoil Shape Optimization in Viscous Flow
NASA Technical Reports Server (NTRS)
Li, Wu
2003-01-01
Simulation results obtained by using FUN2D for robust airfoil shape optimization in transonic viscous flow are included to show the potential of the profile optimization method for generating fairly smooth optimal airfoils with no off-design performance degradation.
Turbidimetric detection method in flow-assisted separation of dispersed samples.
Zattoni, Andrea; Loli Piccolomini, Elena; Torsi, Giancarlo; Reschiglian, Pierluigi
2003-12-01
Characterization of dispersed samples is an outstanding trend in analytical science. Among flow-assisted separation techniques for dispersed samples, size exclusion chromatography, hydrodynamic chromatography, and field-flow fractionation are the most widely applied. With dispersed analytes separated by these techniques, the UV/vis spectrophotometric detectors work as turbidimeters. To directly convert the analytical signal for quantitative analysis, the extinction properties of the dispersed analyte must be known. A new method is proposed to experimentally obtain-by single-run, flow-assisted separation with UV/vis diode-array detectors-the mass-size (or number-size) distribution function of the analytes when a retention-to-size relationship is either theoretically or empirically available for the chosen separation technique. This approach needs neither standards nor reliance on a method to predict the optical properties of the analytes. Theory and original algorithms are presented. Algorithms are then tested to optimize the numerical routines. Accuracy and robustness of the method are evaluated by simulation, and limitations for the application to experimental data are described. Finally, first application to field-flow fractionation shows validity of the method when applied to a few real cases.
NASA Astrophysics Data System (ADS)
Schrijver, Marc; Slump, Cornelis H.; Storm, Corstiaan J.
2001-05-01
Pijls and De Bruyne (1993) developed a method employing intravascular blood pressure gradients to calculate the Myocardial Fractional Flow Reserve (FFR). This flow reserve is a better indication of the functional severity of a coronary stenosis than percentage diameter or luminal area reduction as provided by traditional Quantitative Coronary Angiography (QCA). However, to use this method, all of the relevant artery segments have to be select intra-operatively. After the procedure, only the segments for which a pressure reading is available can be graded. We previously introduced another way to assess the functional severity of stenosis using angiographic projections: the Relative Coronary Flow Reserve (RCFR). It is based on standard densitometric blood velocity and flow reserve methods, but without the need to estimate the geometry of the artery. This paper demonstrates that this RCFR method yields -- in theory -- the same results as the FFR, and can be given an almost identical interpretation. This provides the opportunity to use the RCFR retrospectively, when pressure gradients are not available for the segment(s) of interest.
Towards a segregated time spectral solution method for incompressible viscous flows
NASA Astrophysics Data System (ADS)
Sabine, Baumbach
2016-06-01
Considering the growth of interest in understanding flow phenomena in rotational machines, computational fluid dynamics (CFD) is a powerful tool to reach this goal. Especially unsteady simulations are becoming a focus of interest. Nevertheless, unsteady simulations require huge computational times and ressources, thus it is necessary to investigate other methods to find more appropriate approaches to model time-periodic cases. For time-periodic flows the time spectral method (TSM) presents an interesting alternative to the regular time marching solvers. The TSM is well-known for computation of compressible time-periodic flows, but applications to incompressible cases are limited. This paper presents an extension of the TSM to incompressible flows. While there have been previous implementations using pressure correction method with an explicit treatment of time coupling, here an implicit treatment is chosen. To increase efficiency and employ a more robust coupling of the individual time instances the momentum equations are solved in block-coupled fashion. The pressure correction term is solved segregatedly. To consider cases with dynamic mesh motion an arbitrary lagrange Euler (ALE) formulation is also used in the solver. The efficiency of the method is demonstrated using a basic 2D aerodynamic test case and the results are compared to traditional time-stepping approaches.
Modeling dam-break flows using finite volume method on unstructured grid
Technology Transfer Automated Retrieval System (TEKTRAN)
Two-dimensional shallow water models based on unstructured finite volume method and approximate Riemann solvers for computing the intercell fluxes have drawn growing attention because of their robustness, high adaptivity to complicated geometry and ability to simulate flows with mixed regimes and di...
An improvement of the smoke-wire method of flow visualization
NASA Astrophysics Data System (ADS)
Fukamachi, N.; Ohya, Y.; Nakamura, Y.
1991-01-01
One of the serious drawbacks of the smoke-wire method, which is a simple but very useful means of flow visualization in low-speed wind-tunnel studies, is the short duration of the smoke. In this paper, it is shown that the duration time can be greatly increased by mixing a fine metal powder with the oil.
A spectral-element discontinuous Galerkin lattice Boltzmann method for incompressible flows.
Min, M.; Lee, T.; Mathematics and Computer Science; City Univ. of New York
2011-01-01
We present a spectral-element discontinuous Galerkin lattice Boltzmann method for solving nearly incompressible flows. Decoupling the collision step from the streaming step offers numerical stability at high Reynolds numbers. In the streaming step, we employ high-order spectral-element discontinuous Galerkin discretizations using a tensor product basis of one-dimensional Lagrange interpolation polynomials based on Gauss-Lobatto-Legendre grids. Our scheme is cost-effective with a fully diagonal mass matrix, advancing time integration with the fourth-order Runge-Kutta method. We present a consistent treatment for imposing boundary conditions with a numerical flux in the discontinuous Galerkin approach. We show convergence studies for Couette flows and demonstrate two benchmark cases with lid-driven cavity flows for Re = 400-5000 and flows around an impulsively started cylinder for Re = 550-9500. Computational results are compared with those of other theoretical and computational work that used a multigrid method, a vortex method, and a spectral element model.
A DLM/FD/IB method for simulating compound vesicle motion under creeping flow condition
NASA Astrophysics Data System (ADS)
Pan, Tsorng-Whay; Zhao, Shihai; Niu, Xiting; Glowinski, Roland
2015-11-01
In this article we present first a new distributed Lagrange multiplier/fictitious domain (DLM/FD) method for simulating fluid-particle interaction in Stokes flow. A conjugate gradient method driven by both pressure and distributed Lagrange multiplier, called one-shot method, has been developed to solve the discrete Stokes problem while enforcing the rigid body motion within the region occupied by the particle. The methodology is validated by comparing the numerical results of a neutrally buoyant particle of either a circular or elliptic shape with the associated Jeffery's solutions. We have successively combined the above methodology with an immersed boundary (IB) method and an elastic membrane modeled by a spring network to simulate the dynamics of a compound vesicle. In simple shear flow under creeping flow condition, the results are consistent with those obtained in literature. In Poiseuille flow, the compound vesicle motion is dominated by the motion of the vesicle membrane as expected and stays in the central region of the channel.
NASA Technical Reports Server (NTRS)
Hartill, W. R.
1977-01-01
A hypersonic wind tunnel test method for obtaining credible aerodynamic data on a complete hypersonic vehicle (generic X-24c) with scramjet exhaust flow simulation is described. The general problems of simulating the scramjet exhaust as well as accounting for scramjet inlet flow and vehicle forces are analyzed, and candidate test methods are described and compared. The method selected as most useful makes use of a thrust-minus-drag flow-through balance with a completely metric model. Inlet flow is diverted by a fairing. The incremental effect of the fairing is determined in the testing of two reference models. The net thrust of the scramjet module is an input to be determined in large-scale module tests with scramjet combustion. Force accounting is described, and examples of force component levels are predicted. Compatibility of the test method with candidate wind tunnel facilities is described, and a preliminary model mechanical arrangement drawing is presented. The balance design and performance requirements are described in a detailed specification. Calibration procedures, model instrumentation, and a test plan for the model are outlined.
The Sequential Order of Procedural Instructions: Some Formal Methods for Designers of Flow Charts.
ERIC Educational Resources Information Center
Jansen, Carel J. M.; Steehouder, Michael F.
1996-01-01
States that document designers presenting procedural instructions can choose several formats: prose, table, logical tree, or flow chart. Suggests that instructions should allow users to reach the outcome without losing time. Discusses two formal methods that help determine which order is most efficient--that based on the selection principle, or…
SITE TECHNOLOGY CAPSULE: FILTER FLOW TECHNOLOGY, INC. - COLLOID POLISHING FILTER METHOD
The Filter Flow Technology, Inc. (FFT) Coloid Polishing Filter Method (CPFM) was demonstrated at the U.S Department of Energy's (DOE) Rock Flats Plant (RFP) as part of the U.S. Environmental Protection Agency's (EPA) Superfund and Innovative Technology Evaluation (SITE) program. ...
Application of a Modular Particle-Continuum Method to Partially Rarefied, Hypersonic Flow
NASA Astrophysics Data System (ADS)
Deschenes, Timothy R.; Boyd, Iain D.
2011-05-01
The Modular Particle-Continuum (MPC) method is used to simulate partially-rarefied, hypersonic flow over a sting-mounted planetary probe configuration. This hybrid method uses computational fluid dynamics (CFD) to solve the Navier-Stokes equations in regions that are continuum, while using direct simulation Monte Carlo (DSMC) in portions of the flow that are rarefied. The MPC method uses state-based coupling to pass information between the two flow solvers and decouples both time-step and mesh densities required by each solver. It is parallelized for distributed memory systems using dynamic domain decomposition and internal energy modes can be consistently modeled to be out of equilibrium with the translational mode in both solvers. The MPC results are compared to both full DSMC and CFD predictions and available experimental measurements. By using DSMC in only regions where the flow is nonequilibrium, the MPC method is able to reproduce full DSMC results down to the level of velocity and rotational energy probability density functions while requiring a fraction of the computational time.
Helical swimming in Stokes flow using a novel boundary-element method
NASA Astrophysics Data System (ADS)
Liu, Bin; Breuer, Kenneth S.; Powers, Thomas R.
2013-06-01
We apply the boundary-element method to Stokes flows with helical symmetry, such as the flow driven by an immersed rotating helical flagellum. We show that the two-dimensional boundary integral method can be reduced to one dimension using the helical symmetry. The computational cost is thus much reduced while spatial resolution is maintained. We review the robustness of this method by comparing the simulation results with the experimental measurement of the motility of model helical flagella of various ratios of pitch to radius, along with predictions from resistive-force theory and slender-body theory. We also show that the modified boundary integral method provides reliable convergence if the singularities in the kernel of the integral are treated appropriately.
Hybrid Particle-Continuum Methods for Nonequilibrium Gas and Plasma Flows
Boyd, Iain D.
2011-05-20
Two different hybrid particle-continuum methods are described for simulation of nonequilibrium gas and plasma dynamics. The first technique, used for nonequilibrium hypersonic gas flows, uses either a continuum description or a particle method throughout a flow domain based on local conditions. This technique is successful in reproducing the results of full particle simulations at a small fraction of the cost. The second method uses a continuum model of the electrons combined with a particle description of the ions and atoms for simulating plasma jets. The physical accuracy of the method is assessed through comparisons with plasma plume measurements obtained in space. These examples illustrate that the complex physical phenomena associated with nonequilibrium conditions can be simulated with physical accuracy and numerical efficiency using such hybrid approaches.
NASA Astrophysics Data System (ADS)
Pepona, Marianna; Favier, Julien
2016-09-01
In this work, we propose a numerical framework to simulate fluid flows in interaction with moving porous media of complex geometry. It is based on the Lattice Boltzmann method including porous effects via a Brinkman-Forchheimer-Darcy force model coupled to the Immersed Boundary method to handle complex geometries and moving structures. The coupling algorithm is described in detail and it is validated on well-established literature test cases for both stationary and moving porous configurations. The proposed method is easy to implement and efficient in terms of CPU cost and memory management compared to alternative methods which can be used to deal with moving immersed porous media, e.g. re-meshing at each time step or use of a moving/chimera mesh. An overall good agreement was obtained with reference results, opening the way to the numerical simulation of moving porous media for flow control applications.
Numerical simulation of stratified shear flow using a higher order Taylor series expansion method
Iwashige, Kengo; Ikeda, Takashi
1995-09-01
A higher order Taylor series expansion method is applied to two-dimensional numerical simulation of stratified shear flow. In the present study, central difference scheme-like method is adopted for an even expansion order, and upwind difference scheme-like method is adopted for an odd order, and the expansion order is variable. To evaluate the effects of expansion order upon the numerical results, a stratified shear flow test in a rectangular channel (Reynolds number = 1.7x10{sup 4}) is carried out, and the numerical velocity and temperature fields are compared with experimental results measured by laser Doppler velocimetry thermocouples. The results confirm that the higher and odd order methods can simulate mean velocity distributions, root-mean-square velocity fluctuations, Reynolds stress, temperature distributions, and root-mean-square temperature fluctuations.
A self-adaptive-grid method with application to airfoil flow
NASA Technical Reports Server (NTRS)
Nakahashi, K.; Deiwert, G. S.
1985-01-01
A self-adaptive-grid method is described that is suitable for multidimensional steady and unsteady computations. Based on variational principles, a spring analogy is used to redistribute grid points in an optimal sense to reduce the overall solution error. User-specified parameters, denoting both maximum and minimum permissible grid spacings, are used to define the all-important constants, thereby minimizing the empiricism and making the method self-adaptive. Operator splitting and one-sided controls for orthogonality and smoothness are used to make the method practical, robust, and efficient. Examples are included for both steady and unsteady viscous flow computations about airfoils in two dimensions, as well as for a steady inviscid flow computation and a one-dimensional case. These examples illustrate the precise control the user has with the self-adaptive method and demonstrate a significant improvement in accuracy and quality of the solutions.
Chen, Bor-Sen; Li, Cheng-Wei
2015-01-01
In general, it is very difficult to measure the information flow in a cellular network directly. In this study, based on an information flow model and microarray data, we measured the information flow in cellular networks indirectly by using a systems biology method. First, we used a recursive least square parameter estimation algorithm to identify the system parameters of coupling signal transduction pathways and the cellular gene regulatory network (GRN). Then, based on the identified parameters and systems theory, we estimated the signal transductivities of the coupling signal transduction pathways from the extracellular signals to each downstream protein and the information transductivities of the GRN between transcription factors in response to environmental events. According to the proposed method, the information flow, which is characterized by signal transductivity in coupling signaling pathways and information transductivity in the GRN, can be estimated by microarray temporal data or microarray sample data. It can also be estimated by other high-throughput data such as next-generation sequencing or proteomic data. Finally, the information flows of the signal transduction pathways and the GRN in leukemia cancer cells and non-leukemia normal cells were also measured to analyze the systematic dysfunction in this cancer from microarray sample data. The results show that the signal transductivities of signal transduction pathways change substantially from normal cells to leukemia cancer cells.
Progress Towards a Cartesian Cut-Cell Method for Viscous Compressible Flow
NASA Technical Reports Server (NTRS)
Berger, Marsha; Aftosmis, Michael J.
2012-01-01
We present preliminary development of an approach for simulating high Reynolds number steady compressible flow in two space dimensions using a Cartesian cut-cell finite volume method. We consider both laminar and turbulent flow with both low and high cell Reynolds numbers near the wall. The approach solves the full Navier-Stokes equations in all cells, and uses a wall model to address the resolution requirements near boundaries and to mitigate mesh irregularities in cut cells. We present a quadratic wall model for low cell Reynolds numbers. At high cell Reynolds numbers, the quadratic is replaced with a newly developed analytic wall model stemming from solution of a limiting form of the Spalart-Allmaras turbulence model which features a forward evaluation for flow velocity and exactly matches characteristics of the SA turbulence model in the field. We develop multigrid operators which attain convergence rates similar to inviscid multigrid. Investigations focus on preliminary verification and validation of the method. Flows over flat plates and compressible airfoils show good agreement with both theoretical results and experimental data. Mesh convergence studies on sub- and transonic airfoil flows show convergence of surface pressures with wall spacings as large as approx.0.1% chord. With the current analytic wall model, one or two additional refinements near the wall are required to obtain mesh converged values of skin friction.
An examination of bias in Method 2 measurements under controlled non-axial flow conditions
Norfleet, S.K.; Muzio, L.J.; Martz, T.D.
1997-12-31
Since the installation of continuous emissions monitoring systems (CEMS) under the Acid Rain Rule (40 CFR Part 75), many utilities have found that CEMS are recording consistently higher heat input and SO{sub 2} emissions than conventional methods (input/output and output/loss). This discrepancy, which appears prevalent across the industry, is causing utilities to report greater heat input and SO{sub 2} and CO{sub 2} emissions than are believed to be justified. This paper describes {open_quotes}swirl tunnel{close_quotes} tests recently completed as part of an Electric Power Research Institute (EPRI) research project initiated to identify the cause(s) for high CEMS measurements. The testing was performed in a precision flow facility with custom fabricated swirl vanes for inducing different tangential flow components, variable speed fan control for flow rate adjustment and a venturi section for total flow measurement. Results are presented from tests designed to systematically assess the effect of non-axial flow components on EPA Methods 1 and 2, the relative suitability of alternative multidimensional pitot probes and the relative accuracy of pressure reading instrumentation.
Optimization of the AC-gradient method for velocity profile measurement and application to slow flow
NASA Astrophysics Data System (ADS)
Kartäusch, Ralf; Helluy, Xavier; Jakob, Peter Michael; Fidler, Florian
2014-11-01
This work presents a spectroscopic method to measure slow flow. Within a single shot the velocity distribution is acquired. This allows distinguishing rapidly between single velocities within the sampled volume with a high sensitivity. The technique is based on signal acquisition in the presence of a periodic gradient and a train of refocussing RF pulses. The theoretical model for trapezoidal bipolar pulse shaped gradients under consideration of diffusion and the outflow effect is introduced. A phase correction technique is presented that improves the spectral accuracy. Therefore, flow phantom measurements are used to validate the new sequence and the simulation based on the theoretical model. It was demonstrated that accurate parabolic flow profiles can be acquired and flow variations below 200 μm/s can be detected. Three post-processing methods that eliminate static background signal are also presented for applications in which static background signal dominates. Finally, this technique is applied to flow measurement of a small alder tree demonstrating a typical application of in vivo plant measurements.
NASA Astrophysics Data System (ADS)
Luo, Dahai; Yan, Chao; Wang, Xiaoyong
2015-02-01
Separation commonly exists in the flows around flight vehicles and also in the internal combustor flows. Simulation of high-speed turbulent-separated flows using a reliable computational design tool is crucial for the development of supersonic and hypersonic vehicles. In this paper, we present the computational results of supersonic base and ramped-cavity flows at high Reynolds numbers using the partially averaged Navier-Stokes (PANS) method. The current PANS models are based on the Menter SST turbulence model and also the Wilcox k-ω model. Results from PANS simulations are compared in detail with the available experimental data. The effect of the resolution control parameter fk (the ratio of unresolved-to-total kinetic energy) relevant to the PANS method is investigated. More turbulent flow structures are resolved as expected with decreasing fk, but it does not mean better results can be obtained. Spatially varying and dynamically updated fk in PANS simulations has been performed. Results from variable fk PANS simulations show good agreement with the experiment and great improvement when compared to Reynolds averaged Navier-Stokes (RANS) computation and constant fk PANS simulations.
A high-throughput method for detection of DNA in chloroplasts using flow cytometry
Rowan, Beth A; Oldenburg, Delene J; Bendich, Arnold J
2007-01-01
Background The amount of DNA in the chloroplasts of some plant species has been shown recently to decline dramatically during leaf development. A high-throughput method of DNA detection in chloroplasts is now needed in order to facilitate the further investigation of this process using large numbers of tissue samples. Results The DNA-binding fluorophores 4',6-diamidino-2-phenylindole (DAPI), SYBR Green I (SG), SYTO 42, and SYTO 45 were assessed for their utility in flow cytometric analysis of DNA in Arabidopsis chloroplasts. Fluorescence microscopy and real-time quantitative PCR (qPCR) were used to validate flow cytometry data. We found neither DAPI nor SYTO 45 suitable for flow cytometric analysis of chloroplast DNA (cpDNA) content, but did find changes in cpDNA content during development by flow cytometry using SG and SYTO 42. The latter dye provided more sensitive detection, and the results were similar to those from the fluorescence microscopic analysis. Differences in SYTO 42 fluorescence were found to correlate with differences in cpDNA content as determined by qPCR using three primer sets widely spaced across the chloroplast genome, suggesting that the whole genome undergoes copy number reduction during development, rather than selective reduction/degradation of subgenomic regions. Conclusion Flow cytometric analysis of chloroplasts stained with SYTO 42 is a high-throughput method suitable for determining changes in cpDNA content during development and for sorting chloroplasts on the basis of DNA content. PMID:17381841
Investigation of a New Monte Carlo Method for the Transitional Gas Flow
Luo, X.; Day, Chr.
2011-05-20
The Direct Simulation Monte Carlo method (DSMC) is well developed for rarefied gas flow in transition flow regime when 0.01
Flow method and apparatus for screening chemicals using micro x-ray fluorescence
Warner, Benjamin P.; Havrilla, George J.; Miller, Thomasin C.; Lewis, Cris; Mahan, Cynthia A.; Wells, Cyndi A.
2009-04-14
Method and apparatus for screening chemicals using micro x-ray fluorescence. A method for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder involves flow-separating a solution of chemicals and target binders into separated components, exposing them to an x-ray excitation beam, detecting x-ray fluorescence signals from the components, and determining from the signals whether or not a binding event between a chemical and target binder has occurred.
Flow method and apparatus for screening chemicals using micro x-ray fluorescence
Warner, Benjamin P.; Havrilla, George J.; Miller, Thomasin C.; Lewis, Cris; Mahan, Cynthia A.; Wells, Cyndi A.
2011-04-26
Method and apparatus for screening chemicals using micro x-ray fluorescence. A method for screening a mixture of potential pharmaceutical chemicals for binding to at least one target binder involves flow separating a solution of chemicals and target binders into separated components, exposing them to an x-ray excitation beam, detecting x-ray fluorescence signals from the components, and determining from the signals whether or not a binding event between a chemical and target binder has occurred.
A p-version finite element method for steady incompressible fluid flow and convective heat transfer
NASA Technical Reports Server (NTRS)
Winterscheidt, Daniel L.
1993-01-01
A new p-version finite element formulation for steady, incompressible fluid flow and convective heat transfer problems is presented. The steady-state residual equations are obtained by considering a limiting case of the least-squares formulation for the transient problem. The method circumvents the Babuska-Brezzi condition, permitting the use of equal-order interpolation for velocity and pressure, without requiring the use of arbitrary parameters. Numerical results are presented to demonstrate the accuracy and generality of the method.
Stevens, Fred J.
1992-01-01
A novel method of electric field flow fractionation for separating solute molecules from a carrier solution is disclosed. The method of the invention utilizes an electric field that is periodically reversed in polarity, in a time-dependent, wave-like manner. The parameters of the waveform, including amplitude, frequency and wave shape may be varied to optimize separation of solute species. The waveform may further include discontinuities to enhance separation.
Impact of Energy Slope Averaging Methods on Numerical Solution of 1D Steady Gradually Varied Flow
NASA Astrophysics Data System (ADS)
Artichowicz, Wojciech; Prybytak, Dzmitry
2015-12-01
In this paper, energy slope averaging in the one-dimensional steady gradually varied flow model is considered. For this purpose, different methods of averaging the energy slope between cross-sections are used. The most popular are arithmetic, geometric, harmonic and hydraulic means. However, from the formal viewpoint, the application of different averaging formulas results in different numerical integration formulas. This study examines the basic properties of numerical methods resulting from different types of averaging.
NASA Technical Reports Server (NTRS)
Eberle, A.
1978-01-01
Analysis of the pressure minimum integral in the calculation of three-dimensional potential flow around wings makes it possible to use non-rectangular mesh networks for distributing the three-dimensional potential into discrete points. The method is comparatively easily expanded to the treatment of realistic airplane configurations. Shock-pressure affected pressure distributions on any wings are determined with accuracy using this method.
Methods for estimating selected low-flow frequency statistics for unregulated streams in Kentucky
Martin, Gary R.; Arihood, Leslie D.
2010-01-01
This report provides estimates of, and presents methods for estimating, selected low-flow frequency statistics for unregulated streams in Kentucky including the 30-day mean low flows for recurrence intervals of 2 and 5 years (30Q2 and 30Q5) and the 7-day mean low flows for recurrence intervals of 5, 10, and 20 years (7Q2, 7Q10, and 7Q20). Estimates of these statistics are provided for 121 U.S. Geological Survey streamflow-gaging stations with data through the 2006 climate year, which is the 12-month period ending March 31 of each year. Data were screened to identify the periods of homogeneous, unregulated flows for use in the analyses. Logistic-regression equations are presented for estimating the annual probability of the selected low-flow frequency statistics being equal to zero. Weighted-least-squares regression equations were developed for estimating the magnitude of the nonzero 30Q2, 30Q5, 7Q2, 7Q10, and 7Q20 low flows. Three low-flow regions were defined for estimating the 7-day low-flow frequency statistics. The explicit explanatory variables in the regression equations include total drainage area and the mapped streamflow-variability index measured from a revised statewide coverage of this characteristic. The percentage of the station low-flow statistics correctly classified as zero or nonzero by use of the logistic-regression equations ranged from 87.5 to 93.8 percent. The average standard errors of prediction of the weighted-least-squares regression equations ranged from 108 to 226 percent. The 30Q2 regression equations have the smallest standard errors of prediction, and the 7Q20 regression equations have the largest standard errors of prediction. The regression equations are applicable only to stream sites with low flows unaffected by regulation from reservoirs and local diversions of flow and to drainage basins in specified ranges of basin characteristics. Caution is advised when applying the equations for basins with characteristics near the
NASA Astrophysics Data System (ADS)
Rakhmanov, Vitaly V.; Kulikov, Dmitry V.
2014-08-01
Possibility of use of a refractive-index-matching method for flow investigation by LDA method in models of the fire chambers of complex geometry is shown. The technique of flows investigation by LDA method is developed. The given technique can be successfully applied in leading branches of a thermal and hydropower engineering, in case of need of flows diagnostics in models of devices with the complex geometry.
Research on target tracking in coal mine based on optical flow method
NASA Astrophysics Data System (ADS)
Xue, Hongye; Xiao, Qingwei
2015-03-01
To recognize, track and count the bolting machine in coal mine video images, a real-time target tracking method based on the Lucas-Kanade sparse optical flow is proposed in this paper. In the method, we judge whether the moving target deviate from its trajectory, predicate and correct the position of the moving target. The method solves the problem of failure to track the target or lose the target because of the weak light, uneven illumination and blocking. Using the VC++ platform and Opencv lib we complete the recognition and tracking. The validity of the method is verified by the result of the experiment.