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Sample records for flow cytometry-based methods

  1. Establishment and application of a flow cytometry-based method for detecting histone acetylation levels.

    PubMed

    Jianhui, Li; Chunfu, Wang; Fan, Bai; Yan, Zhuang; Zhuojun, Mao; Yongtao, Sun

    2016-06-20

    Histone deacetylase inhibitors, which have also received attention in AIDS and other diseases, are a new class of anticancer drugs developed in recent years. However, there is still a lack of a unified and reliable method for detecting histone acetylation levels in basic and clinical research. In this study, we developed a flow cytometry-based method to detect histone acetylation levels by comparing different sample processing temperature (on ice vs. room temperature), permeabilization method (intracellular vs. nuclear), antibody dose (antibody titration) and antibody incubation time (time gradient) using whole blood and peripheral blood mononuclear cells. In addition, we applied this optimized method in in vitro experiment and clinical trial of Chidamide (the only China FDA approved HDACi), the result of which confirmed that the flow cytometry-based method for detecting histone acetylation levels is a reliable, fast and convenient method which can be used in basic and clinical research.

  2. Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates.

    PubMed

    Meyer, Christine; Haberthur, Kristen; Asquith, Mark; Messaoudi, Ilhem

    2015-01-01

    Flow cytometry is an invaluable technique that can be used to phenotypically and functionally characterize immune cell populations ex vivo. This technology has greatly advanced our ability to gain critical insight into age-related changes in immune function, commonly known as immune senescence. Rodents have been traditionally used to investigate the molecular mechanisms of immune senescence because they offer the distinct advantages of an extensive set of reagents, the presence of genetically modified strains, and a short lifespan that allows for longevity studies of short duration. More recently, nonhuman primates (NHPs), and specifically rhesus macaques, have emerged as a leading translational model to study various aspects of human aging. In contrast to rodents, they share significant genetic homology as well as physiological and behavioral characteristics with humans. Furthermore, rhesus macaques are a long-lived outbred species, which makes them an ideal translational model. Therefore, NHPs offer a unique opportunity to carry out mechanistic studies under controlled laboratory conditions (e.g., photoperiod, temperature, diet, and medications) in a species that closely mimics human biology. Moreover similar techniques (e.g., activity recording and MRI) can be used to measure physiological parameters in NHPs, making direct comparisons between NHP and human data sets possible. In addition, the outbred genetics of NHPs enables rigorous validation of research findings that goes beyond proof of principle. Finally, self-selection bias that is often unavoidable in human clinical trials can be completely eliminated with NHP studies. Here we describe flow cytometry-based methods to phenotypically and functionally characterize innate immune cells as well as T and B lymphocyte subsets from isolated peripheral blood mononuclear cells (PBMC) in rhesus macaques.

  3. Flow cytometry based profiling of leukocytes: A new method for diagnosis of tropical theileriosis in crossbred cattle

    PubMed Central

    Jagtap, Ramesh B.; Gupta, Amit; Chaphalkar, Sushama R.

    2015-01-01

    Aim: In India, dairy industries are important for the livelihood of small scale farmers and dairy owners. Tropical theileriosis, mostly affecting dairy cattle and buffaloes is a major threat to dairy and related industries. Tropical theileriosis is caused by Theileria annulata, a hemoprotozoan parasite transmitted by Ixodid ticks of Hyalomma spp. In the present study, we examined the clinical signs, hematological parameters and flow cytometric profile of whole blood in 30 theileriosis affected crossbred cattle. The aim of our study is to analyze, in comparison with clinical and hematological diagnosis, whether flow cytometry based profiling of leukocytes could be used as better, quick and alternative method for diagnosis and screening of bovine tropical theileriosis in crossbred cattle. Materials and Methods: In this study, we screened parasites in 30 peripheral blood samples from clinical cases of theileriosis by Giemsa’s staining technique in crossbred cattle. Hematological analysis was done to estimate hemoglobin (Hb) content, total red blood cell (RBC) count, total leukocyte count and differential leukocyte count. Further, flow cytometric analysis of whole blood was carried out to study leukocytes profile in affected cattle. Results: Microscopic examination of stained blood films revealed the presence of piroplasms in erythrocytes and schizonts in lymphocytes. Hematological examination revealed significant (p<0.05) decrease of Hb percent (Hb %), reduced total RBC and total leukocytes, lymphocytosis, eosinopenia, and neutropenia compared to that of apparently healthy cattle. Flow cytometric profiling of leukocytes revealed the severe effect on shape, size, and granularity of leukocytes, marked decrease in granulocytes and 3-5 fold increase in lymphocytes count compared to clinically healthy cattle. Thus, in both methods, namely conventional and flow cytometric analysis, marked lymphocytosis and decrease in other blood cell counts were observed compared to

  4. A flow-cytometry-based method for detecting simultaneously five allergens in a complex food matrix.

    PubMed

    Otto, Gaetan; Lamote, Amandine; Deckers, Elise; Dumont, Valery; Delahaut, Philippe; Scippo, Marie-Louise; Pleck, Jessica; Hillairet, Caroline; Gillard, Nathalie

    2016-12-01

    To avoid carry-over contamination with allergens, food manufacturers implement quality control strategies relying primarily on detection of allergenic proteins by ELISA. Although sensitive and specific, this method allowed detection of only one allergen per analysis and effective control policies were thus based on multiplying the number of tests done in order to cover the whole range of allergens. We present in this work an immunoassay for the simultaneous detection of milk, egg, peanut, mustard and crustaceans in cookies samples. The method was based on a combination of flow cytometry with competitive ELISA where microbeads were used as sorbent surface. The test was able to detect the presence of the five allergens with median inhibitory concentrations (IC50) ranging from 2.5 to 15 mg/kg according to the allergen to be detected. The lowest concentrations of contaminants inducing a significant difference of signal between non-contaminated controls and test samples were 2 mg/kg of peanut, 5 mg/kg of crustaceans, 5 mg/kg of milk, 5 mg/kg of mustard and 10 mg/kg of egg. Assay sensitivity was influenced by the concentration of primary antibodies added to the sample extract for the competition and by the concentration of allergenic proteins bound to the surface of the microbeads.

  5. A novel high-throughput multi-parameter flow cytometry based method for monitoring and rapid characterization of microbiome dynamics in anaerobic systems.

    PubMed

    Dhoble, Abhishek S; Bekal, Sadia; Dolatowski, William; Yanz, Connor; Lambert, Kris N; Bhalerao, Kaustubh D

    2016-11-01

    A novel multidimensional flow cytometry based method has been demonstrated to monitor and rapidly characterize the dynamics of the complex anaerobic microbiome associated with perturbations in external environmental factors. While community fingerprinting provides an estimate of the meta genomic structure, flow cytometry provides a fingerprint of the community morphology including its autofluorescence spectrum in a high-throughput manner. Using anaerobic microbial consortia perturbed with the controlled addition of various carbon sources, it is possible to quantitatively discriminate between divergent microbiome analogous to community fingerprinting techniques using automated ribosomal intergenic spacer analysis (ARISA). The utility of flow cytometry based method has also been demonstrated in a fully functional industry scale anaerobic digester to distinguish between microbiome composition caused by varying hydraulic retention time (HRT). This approach exploits the rich multidimensional information from flow cytometry for rapid characterization of the dynamics of microbial communities.

  6. A Flow Cytometry-based Method for the Screening of Nanomaterial-induced Reactive Oxygen Species Production in Leukocytes Subpopulations in Whole Blood.

    PubMed

    Kermanizadeh, Ali; Jantzen, Kim; Brown, David M; Møller, Peter; Loft, Steffen

    2017-07-10

    To date, the use and translation of nanomedicine from the laboratory to the clinic has been relatively slow. Among other issues, one of the reasons for this tardiness is the lack of the availability of quick and reliable toxicity tools for the screening of nanomaterials (NMs). In this investigation, we apply a flow cytometry-based method for the detection of nanomaterial-induced oxidative stress by measurement of reactive oxygen species production in specific leukocyte subpopulations in human whole blood. The screening of a panel of relevant nanomedical-associated materials (liposomes, silica, iron oxide and functionalized single-walled carbon nanotubes) demonstrated that only the carbon nanotubes induced oxidative stress in human circulating leukocytes. In summary, we apply and corroborate a flow cytometry-based method for the simple and effective measurement of NM-induced oxidative stress in human blood subpopulations after realistic and relevant exposure scenarios which is extremely useful in future toxicological applications. © 2017 Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society).

  7. Development of a Flow Cytometry-Based Method for Rapid Detection of Escherichia coli and Shigella Spp. Using an Oligonucleotide Probe

    PubMed Central

    Xue, Yong; Wilkes, Jon G.; Moskal, Ted J.; Williams, Anna J.; Cooper, Willie M.; Nayak, Rajesh; Rafii, Fatemeh; Buzatu, Dan A.

    2016-01-01

    Standard methods to detect Escherichia coli contamination in food use the polymerase chain reaction (PCR) and agar culture plates. These methods require multiple incubation steps and take a long time to results. An improved rapid flow-cytometry based detection method was developed, using a fluorescence-labeled oligonucleotide probe specifically binding a16S rRNA sequence. The method positively detected 51 E. coli isolates as well as 4 Shigella species. All 27 non-E. coli strains tested gave negative results. Comparison of the new genetic assay with a total plate count (TPC) assay and agar plate counting indicated similar sensitivity, agreement between cytometry cell and colony counts. This method can detect a small number of E.coli cells in the presence of large numbers of other bacteria. This method can be used for rapid, economical, and stable detection of E. coli and Shigella contamination in the food industry and other contexts. PMID:26913737

  8. Representation Method for Spectrally Overlapping Signals in Flow Cytometry Based on Fluorescence Pulse Time-Delay Estimation

    PubMed Central

    Zhang, Wenchang; Lou, Xiaoping; Meng, Xiaochen; Zhu, Lianqing

    2016-01-01

    Flow cytometry is being applied more extensively because of the outstanding advantages of multicolor fluorescence analysis. However, the intensity measurement is susceptible to the nonlinearity of the detection method. Moreover, in multicolor analysis, it is impossible to discriminate between fluorophores that spectrally overlap; this influences the accuracy of the fluorescence pulse signal representation. Here, we focus on spectral overlap in two-color analysis, and assume that the fluorescence follows the single exponential decay model. We overcome these problems by analyzing the influence of the spectral overlap quantitatively, which enables us to propose a method of fluorescence pulse signal representation based on time-delay estimation (between fluorescence and scattered pulse signals). First, the time delays are estimated using a modified chirp Z-transform (MCZT) algorithm and a fine interpolation of the correlation peak (FICP) algorithm. Second, the influence of hardware is removed via calibration, in order to acquire the original fluorescence lifetimes. Finally, modulated signals containing phase shifts associated with these lifetimes are created artificially, using a digital signal processing method, and reference signals are introduced in order to eliminate the influence of spectral overlap. Time-delay estimation simulation and fluorescence signal representation experiments are conducted on fluorescently labeled cells. With taking the potentially overlap of autofluorescence as part of the observed fluorescence spectrum, rather than distinguishing the individual influence, the results show that the calculated lifetimes with spectral overlap can be rectified from 8.28 and 4.86 ns to 8.51 and 4.63 ns, respectively, using the comprehensive approach presented in this work. These values agree well with the lifetimes (8.48 and 4.67 ns) acquired for cells stained with single-color fluorochrome. Further, these results indicate that the influence of spectral

  9. Representation Method for Spectrally Overlapping Signals in Flow Cytometry Based on Fluorescence Pulse Time-Delay Estimation.

    PubMed

    Zhang, Wenchang; Lou, Xiaoping; Meng, Xiaochen; Zhu, Lianqing

    2016-11-23

    Flow cytometry is being applied more extensively because of the outstanding advantages of multicolor fluorescence analysis. However, the intensity measurement is susceptible to the nonlinearity of the detection method. Moreover, in multicolor analysis, it is impossible to discriminate between fluorophores that spectrally overlap; this influences the accuracy of the fluorescence pulse signal representation. Here, we focus on spectral overlap in two-color analysis, and assume that the fluorescence follows the single exponential decay model. We overcome these problems by analyzing the influence of the spectral overlap quantitatively, which enables us to propose a method of fluorescence pulse signal representation based on time-delay estimation (between fluorescence and scattered pulse signals). First, the time delays are estimated using a modified chirp Z-transform (MCZT) algorithm and a fine interpolation of the correlation peak (FICP) algorithm. Second, the influence of hardware is removed via calibration, in order to acquire the original fluorescence lifetimes. Finally, modulated signals containing phase shifts associated with these lifetimes are created artificially, using a digital signal processing method, and reference signals are introduced in order to eliminate the influence of spectral overlap. Time-delay estimation simulation and fluorescence signal representation experiments are conducted on fluorescently labeled cells. With taking the potentially overlap of autofluorescence as part of the observed fluorescence spectrum, rather than distinguishing the individual influence, the results show that the calculated lifetimes with spectral overlap can be rectified from 8.28 and 4.86 ns to 8.51 and 4.63 ns, respectively, using the comprehensive approach presented in this work. These values agree well with the lifetimes (8.48 and 4.67 ns) acquired for cells stained with single-color fluorochrome. Further, these results indicate that the influence of spectral

  10. Flow cytometry-based methods for assessing soluble scFv activities and detecting pathogen antigens in solution

    SciTech Connect

    Gray, Sean; Weigel, Kris M.; Miller, Keith D.; Ndung'u, Joseph; Buscher, Philippe; Tran, Thao N.; Baird, Cheryl L.; Cangelosi, Gerard A.

    2010-04-01

    Novel methods are reported for evaluating and utilizing single chain fragment variable (scFv) antibodies derived from yeast-display libraries. Yeast-display was used to select scFv specific to invariant surface glycoproteins (ISG) of Trypanosoma brucei. A limiting step in the isolation of scFv from nonimmune libraries is the conversion of highly active yeast-displayed scFv into soluble antibodies that can be used in standard immunoassays. Challenges include limited solubility or activity following secretion and purification of scFv. For this reason, few scFv derived from yeast-display platforms have moved into development and implementation as diagnostic reagents. To address this problem, assays were developed that employ both yeastdisplayed and secreted scFv as analytical reagents. The first is a competitive inhibition flow cytometry (CIFC) assay that detects secreted scFv by virtue of its ability to competitively inhibit the binding of biotinylated antigen to yeast-displayed scFv. The second is an epitope binning assay that uses secreted scFv toidentify additional yeast-displayed scFv that bind nonoverlapping or noncompeting epitopes on an antigen. The epitope binning assay was used not only to identify sandwich assay pairs with yeast-displayed scFv, but also to identify active soluble scFv present in low concentration in a crude expression extract. Finally, a CIFC assay was developed that bypasses entirely the need for soluble scFv expression, by using yeast displayed scFv to detect unlabeled antigen in samples. These methods will facilitate the continued development and practical implementation of scFv derived from yeast-display libraries.

  11. A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum.

    PubMed

    Bei, Amy K; Desimone, Tiffany M; Badiane, Aida S; Ahouidi, Ambroise D; Dieye, Tandakha; Ndiaye, Daouda; Sarr, Ousmane; Ndir, Omar; Mboup, Souleymane; Duraisingh, Manoj T

    2010-04-01

    Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion.

  12. Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia.

    PubMed

    Dasgupta, Swati; Ray, Ujjal K; Mitra, Arpita Ghosh; Bhattacharyya, Deboshree M; Mukhopadhyay, Ashis; Das, Priyabrata; Gangopadhyay, Sudeshna; Roy, Sudip; Mukhopadhyay, Soma

    2017-06-01

    Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitoring of CML. Previously, many technologies, most of which are laborious and time consuming, have been developed to detect BCR-ABL chimeric gene or chromosome. A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion proteins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantitative real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.

  13. Flow cytometry-based DNA hybridization and polymorphism analysis

    SciTech Connect

    Cai, H.; Kommander, K.; White, P.S.; Nolan, J.P.

    1998-07-01

    Functional analysis of the humane genome, including the quantification of differential gene expression and the identification of polymorphic sites and disease genes, is an important element of the Human Genome Project. Current methods of analysis are mainly gel-based assays that are not well-suited to rapid genome-scale analyses. To analyze DNA sequence on a large scale, robust and high throughput assays are needed. The authors are developing a suite of microsphere-based approaches employing fluorescence detection to screen and analyze genomic sequence. The approaches include competitive DNA hybridization to measure DNA or RNA targets in unknown samples, and oligo ligation or extension assays to analyze single-nucleotide polymorphisms. Apart from the advances of sensitivity, simplicity, and low sample consumption, these flow cytometric approaches have the potential for high throughput multiplexed analysis using multicolored microspheres and automated sample handling.

  14. Flow-cytometry-based DNA hybidization and polymorphism analysis

    NASA Astrophysics Data System (ADS)

    Cai, Hong; Kommander, Kristina; White, P. S.; Nolan, John P.

    1998-05-01

    Functional analysis of the human genome, including the quantification of differential gene expression and the identification of polymorphic sites and disease genes, is an important element of the Human Genome Project. Current methods of analysis are mainly gel-based assays that are not well- suited to rapid genome-scale analyses. To analyze DNA sequence on a large scale, robust and high throughput assays are needed. We are developing a suite of microsphere-based approaches employing fluorescence detection to screen and analyze genomic sequence. Our approaches include competitive DNA hybridization to measure DNA or RNA targets in unknown samples, and oligo ligation or extension assays to analyze single-nucleotide polymorphisms. Apart from the advantages of sensitivity, simplicity, and low sample consumption, these flow cytometric approaches have the potential for high throughput multiplexed analysis using multicolored microspheres and automated sample handling.

  15. Detecting endotoxin with a flow cytometry-based magnetic aptasensor.

    PubMed

    Zuo, Ming-Yan; Chen, Li-Juan; Jiang, Hao; Tan, Lin; Luo, Zhao-Feng; Wang, Yan-Mei

    2014-12-01

    Endotoxin, which is also known as lipopolysaccharide (LPS), is a marker for intruding gram-negative pathogens. It is essential to detect endotoxin quickly and sensitively in a complex milieu. A new flow cytometry (FCM)-based magnetic aptasensor assay that employs two endotoxin-binding aptamers and magnetic beads has been developed to detect endotoxin. The endotoxin-conjugated sandwich complex on magnetic beads was observed by scanning confocal laser microscopy. The resulting magnetic aptasensor rapidly detected (<1 min) endotoxin within a broad dynamic detection range of 10(-8) to 10(0)mg/ml in the presence of bovine serum albumin (BSA), RNA, sucrose, and glucose, which are most likely to coexist with endotoxin in the majority of biological liquids. Only 2 μl of magnetic aptasensor was required to quantify the endotoxin solution. Furthermore, the magnetic aptasensor could be regenerated seven times and still presented an outstanding response to the endotoxin solution. Therefore, the magnetic aptasensor exhibited high sensitivity, selectivity, and reproducibility, thereby serving as a powerful tool for the quality control and high-throughput detection of endotoxin in the food and pharmaceutical industries.

  16. Improved signal recovery for flow cytometry based on ‘spatially modulated emission’

    NASA Astrophysics Data System (ADS)

    Quint, S.; Wittek, J.; Spang, P.; Levanon, N.; Walther, T.; Baßler, M.

    2017-09-01

    Recently, the technique of ‘spatially modulated emission’ has been introduced (Baßler et al 2008 US Patent 0080181827A1; Kiesel et al 2009 Appl. Phys. Lett. 94 041107; Kiesel et al 2011 Cytometry A 79A 317-24) improving the signal-to-noise ratio (SNR) for detecting bio-particles in the field of flow cytometry. Based on this concept, we developed two advanced signal processing methods which further enhance the SNR and selectivity for cell detection. The improvements are achieved by adapting digital filtering methods from RADAR technology and mainly address inherent offset elimination, increased signal dynamics and moreover reduction of erroneous detections due to processing artifacts. We present a comprehensive theory on SNR gain and provide experimental results of our concepts.

  17. A flow cytometry-based dopamine transporter binding assay using antagonist-conjugated quantum dots

    SciTech Connect

    Kovtun, Oleg; Ross, Emily J.; Tomlinson, Ian D.; Rosenthal, Sandra J.

    2012-04-05

    Here we present the development and validation of a flow cytometry-based dopamine transporter (DAT) binding assay that uses antagonist-conjugated quantum dots (QDs). Our anticipation is that our QD-based assay is of immediate value to the high throughput screening of novel DAT modulators.

  18. Validation of a Flow Cytometry Based Binding Assay for Evaluation of Monoclonal Antibody Recognizing EGF Receptor

    PubMed Central

    Cedeño-Arias, Mercedes; Sánchez-Ramírez, Javier; Blanco-Santana, Rancés; Rengifo-Calzado, Enrique

    2011-01-01

    An ideal test used to characterize a product must be appropriate for the measurement of product quality, manufacturing consistency, product stability, and comparability studies. Flow cytometry has been successfully applied to the examination of antibodies and receptors on membrane surfaces; however, to date, the analytical validation of cytometry based assays is limited. Here we report on the validation of a flow cytometry-based assay used in the evaluation of nimotuzumab binding to cells over-expressing EGFR on cell surface. The assay was validated by examining, assay robustness, specificity, repeatability and intermediate precision. The assay was highly specific, robust for all studied factors except for cell fixation with 1% paraformaldehyde and met criteria for precision with RSD < 2%. In addition the assay has stability-indicating properties evidenced by the ability to detect changes in mAb degraded samples. Most importantly, the assay demonstrated to be useful for its intended use. PMID:21886904

  19. Flow Cytometry-based Assay for the Monitoring of NK Cell Functions.

    PubMed

    Tognarelli, Sara; Jacobs, Benedikt; Staiger, Nina; Ullrich, Evelyn

    2016-10-30

    Natural killer (NK) cells are an important part of the human tumor immune surveillance system. NK cells are able to distinguish between healthy and virus-infected or malignantly transformed cells due to a set of germline encoded inhibitory and activating receptors. Upon virus or tumor cell recognition a variety of different NK cell functions are initiated including cytotoxicity against the target cell as well as cytokine and chemokine production leading to the activation of other immune cells. It has been demonstrated that accurate NK cell functions are crucial for the treatment outcome of different virus infections and malignant diseases. Here a simple and reliable method is described to analyze different NK cell functions using a flow cytometry-based assay. NK cell functions can be evaluated not only for the whole NK cell population, but also for different NK cell subsets. This technique enables scientists to easily study NK cell functions in healthy donors or patients in order to reveal their impact on different malignancies and to further discover new therapeutic strategies.

  20. In vitro flow cytometry-based screening platform for cellulase engineering

    PubMed Central

    Körfer, Georgette; Pitzler, Christian; Vojcic, Ljubica; Martinez, Ronny; Schwaneberg, Ulrich

    2016-01-01

    Ultrahigh throughput screening (uHTS) plays an essential role in directed evolution for tailoring biocatalysts for industrial applications. Flow cytometry-based uHTS provides an efficient coverage of the generated protein sequence space by analysis of up to 107 events per hour. Cell-free enzyme production overcomes the challenge of diversity loss during the transformation of mutant libraries into expression hosts, enables directed evolution of toxic enzymes, and holds the promise to efficiently design enzymes of human or animal origin. The developed uHTS cell-free compartmentalization platform (InVitroFlow) is the first report in which a flow cytometry-based screened system has been combined with compartmentalized cell-free expression for directed cellulase enzyme evolution. InVitroFlow was validated by screening of a random cellulase mutant library employing a novel screening system (based on the substrate fluorescein-di-β-D-cellobioside), and yielded significantly improved cellulase variants (e.g. CelA2-H288F-M1 (N273D/H288F/N468S) with 13.3-fold increased specific activity (220.60 U/mg) compared to CelA2 wildtype: 16.57 U/mg). PMID:27184298

  1. A novel flow cytometry-based tool for determining the efficiency of human cytomegalovirus infection in THP-1 derived macrophages.

    PubMed

    Li, Huifen; Mao, Genxiang; Carlson, Joshua; Leng, Sean X

    2015-09-01

    Human cytomegalovirus (hCMV) is a ubiquitous pathogen that causes congenital infection and severe infections in immunocompromised patients. Chronic hCMV infection may also play an important role in immunosenescence and adverse health outcomes in older adults. THP-1, a human monocytic cell line and its derived macrophages serve as a useful cell culture model for mechanistic studies of hCMV infection and its underlying biology. A major methodological challenge is the lack of a quick and reliable tool to accurately determine the efficiency of hCMV infection in THP-1 derived macrophages. In this study, we developed a flow cytometry based method using commercially available monoclonal antibody (MAb) against hCMV immediate early (IE) antigen that can accurately determine infection efficiency. We used 0.5% formaldehyde for fixation, 90% methanol for permeabilization, and incubation with FITC conjugated MAb at 37°C. The method was tested by hCMV infection with laboratory Towne strain in the presence or absence of hydrocortisone. It was also compared with the routine flow cytometry protocol using Cytofix/Cytoperm solution and with immunofluorescence. The results indicate that this new method is reliable and time saving for accurate determination of infection efficiency. It may facilitate further investigations into the underlying biological mechanisms of hCMV infection.

  2. Application of an optimized flow cytometry-based quantification of Platelet Activation (PACT): Monitoring platelet activation in platelet concentrates

    PubMed Central

    Roest, Mark; Henskens, Yvonne M. C.; de Laat, Bas; Huskens, Dana

    2017-01-01

    Background Previous studies have shown that flow cytometry is a reliable test to quantify platelet function in stored platelet concentrates (PC). It is thought that flow cytometry is laborious and hence expensive. We have optimized the flow cytometry-based quantification of agonist induced platelet activation (PACT) to a labor, time and more cost-efficient test. Currently the quality of PCs is only monitored by visual inspection, because available assays are unreliable or too laborious for use in a clinical transfusion laboratory. Therefore, the PACT was applied to monitor PC activation during storage. Study design and methods The optimized PACT was used to monitor 5 PCs during 10 days of storage. In brief, optimized PACT uses a ready-to-use reaction mix, which is stable at -20°C. When needed, a test strip is thawed and platelet activation is initiated by mixing PC with PACT. PACT was based on the following agonists: adenosine diphosphate (ADP), collagen-related peptide (CRP) and thrombin receptor-activating peptide (TRAP-6). Platelet activation was measured as P-selectin expression. Light transmission aggregometry (LTA) was performed as a reference. Results Both PACT and LTA showed platelet function decline during 10-day storage after stimulation with ADP and collagen/CRP; furthermore, PACT showed decreasing TRAP-induced activation. Major differences between the two tests are that PACT is able to measure the status of platelets in the absence of agonists, and it can differentiate between the number of activated platelets and the amount of activation, whereas LTA only measures aggregation in response to an agonist. Also, PACT is more time-efficient compared to LTA and allows high-throughput analysis. Conclusion PACT is an optimized platelet function test that can be used to monitor the activation of PCs. PACT has the same accuracy as LTA with regard to monitoring PCs, but it is superior to both LTA and conventional flow cytometry based tests with regard to labor

  3. Quantification of Al2O3 nanoparticles in human cell lines applying inductively coupled plasma mass spectrometry (neb-ICP-MS, LA-ICP-MS) and flow cytometry-based methods.

    PubMed

    Böhme, Steffi; Stärk, Hans-Joachim; Meißner, Tobias; Springer, Armin; Reemtsma, Thorsten; Kühnel, Dana; Busch, Wibke

    2014-01-01

    In order to quantify and compare the uptake of aluminum oxide nanoparticles of three different sizes into two human cell lines (skin keratinocytes (HaCaT) and lung epithelial cells (A549)), three analytical methods were applied: digestion followed by nebulization inductively coupled plasma mass spectrometry (neb-ICP-MS), direct laser ablation ICP-MS (LA-ICP-MS), and flow cytometry. Light and electron microscopy revealed an accumulation and agglomeration of all particle types within the cell cytoplasm, whereas no particles were detected in the cell nuclei. The internalized Al2O3 particles exerted no toxicity in the two cell lines after 24 h of exposure. The smallest particles with a primary particle size (xBET) of 14 nm (Alu1) showed the lowest sedimentation velocity within the cell culture media, but were calculated to have settled completely after 20 h. Alu2 (xBET = 111 nm) and Alu3 (xBET = 750 nm) were calculated to reach the cell surface after 7 h and 3 min, respectively. The internal concentrations determined with the different methods lay in a comparable range of 2-8 µg Al2O3/cm(2) cell layer, indicating the suitability of all methods to quantify the nanoparticle uptake. Nevertheless, particle size limitations of analytical methods using optical devices were demonstrated for LA-ICP-MS and flow cytometry. Furthermore, the consideration and comparison of particle properties as parameters for particle internalization revealed the particle size and the exposure concentration as determining factors for particle uptake.

  4. Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria

    PubMed Central

    Correia, Rodolfo Patussi; Bento, Laiz Cameirão; Bortolucci, Ana Carolina Apelle; Alexandre, Anderson Marega; Vaz, Andressa da Costa; Schimidell, Daniela; Pedro, Eduardo de Carvalho; Perin, Fabricio Simões; Nozawa, Sonia Tsukasa; Mendes, Cláudio Ernesto Albers; Barroso, Rodrigo de Souza; Bacal, Nydia Strachman

    2016-01-01

    ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones. PMID:27759825

  5. Multicolor flow cytometry-based cellular phenotyping identifies osteoprogenitors and inflammatory cells in the osteoarthritic subchondral bone marrow compartment.

    PubMed

    Pippenger, B E; Duhr, R; Muraro, M G; Pagenstert, G I; Hügle, T; Geurts, J

    2015-11-01

    The cellular component of subchondral bone is thought to be responsible for aberrant bone remodeling in osteoarthritis (OA). Direct phenotypical analysis of the cellular compartment is critical to better understand the OA disease process. This study provides proof-of-principle for flow cytometry-based phenotyping of isolated subchondral trabecular bone (STB) marrow cells without prior use of cell culture techniques. Tibial plateaus were obtained from OA patients undergoing total knee arthroplasty. Subchondral bone chips were digested with collagenase IA and single cell suspensions were directly phenotyped using flow cytometry. Cells were analyzed for the expression of alkaline phosphatase (ALP) as osteoblast/osteoprogenitor marker and monocyte/macrophage markers (CD14, CD68, HLA-DR, CD115). MTT staining revealed abundant viable cells in the bone marrow compartment of STB prior to digestion, which were efficiently released by collagenase. Within the CD45-negative cell fraction, approximately 20% of the cells were positive for the early osteoblast/osteoprogenitor marker ALP. Within the CD45+ hematopoietic cell fraction, the majority of cells were of monocytic origin (>80%) displaying strong surface expression of CD14. Discreet macrophage populations (CD14+/HLA-DR+/CD68+) and putative osteoclast progenitors (CD45+/HLA-DR-/CD115+) were unequivocally identified. Osteoblast, macrophage and osteoclast progenitor presence in the subchondral bone unit (SBU) was confirmed by (immuno)histochemical staining for osteocalcin, CD68 and tartrate-resistant acid phosphatase, respectively. Flow cytometric analysis is a valuable methodology to study the cellular compartment of STB marrow. This method provides a proof of principle that the whole resident cell population can be directly phenotypically characterized without the prior use of cell culture techniques. Copyright © 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

  6. A flow cytometry-based assay for the evaluation of antibody-dependent cell-mediated cytotoxicity (ADCC) in cancer cells.

    PubMed

    Salinas-Jazmín, Nohemí; Hisaki-Itaya, Emiliano; Velasco-Velázquez, Marco A

    2014-01-01

    The development of new therapeutic monoclonal antibodies (mAbs) for cancer therapy will rise in the following years. The evaluation of biological activity of mAbs is required during drug development and during drug production as quality control test. Antibody-dependent cell-mediated cytotoxicity (ADCC) is a desirable activity of anticancer mAbs. Here, we describe a flow cytometry-based method to quantify ADCC that combines the staining of cancer cells, effector cells, and dead cells, with specific dyes. This method is inexpensive, has low background, and avoids the use of radioisotopes.

  7. Oxygen Enhancement Ratio in Radiation-Induced Initial DSBs by an Optimized Flow Cytometry-based Gamma-H2AX Analysis in A549 Human Cancer Cells.

    PubMed

    Sunada, Shigeaki; Hirakawa, Hirokazu; Fujimori, Akira; Uesaka, Mitsuru; Okayasu, Ryuichi

    2017-08-22

    High-linear energy transfer (LET) heavy ions cause higher therapeutic effects than low-LET radiation due to lower dependency on oxygen concentration in tumor cell killing. The lethality after irradiation largely depends on DNA double-strand breaks (DSBs), however the detailed LET dependency for DSB induction under oxic and hypoxic conditions has not been reported. Therefore, we evaluated the oxygen enhancement ratio (OER) of heavy ion-induced DSB induction using a highly-optimized flow cytometry-based method of γ-H2AX detection. Non-small cell lung cancer (NSCLC) A549 cells were exposed to X-ray, carbon-ion and iron-ion radiations under oxic or hypoxic condition. As a DSB marker, the γ-H2AX signal was measured 1 h postirradiation and analyzed by flow cytometry. DSB slope values were calculated as DSB induction per Gy. Our method was able to detect high-LET radiation-induced DSBs even from clustered DNA damage sites. We also showed a decrease in OER value in an LET-dependent manner regardless of radiation type. In summary, we demonstrated a simple, quick and highly-optimized flow cytometry-based method of DSB analysis that detects DSBs induced by heavy-ion radiation for hypoxic and nonhypoxic cancer cells. Our study may provide a useful biological basis for heavy-ion radiotherapy.

  8. A novel flow cytometry-based assay for the quantification of antibody-dependent pneumococcal agglutination

    PubMed Central

    van der Gaast—de Jongh, Christa E.; Diavatopoulos, Dimitri A.; de Jonge, Marien I.

    2017-01-01

    The respiratory pathogen Streptococcus pneumoniae is a major cause of diseases such as otitis media, pneumonia, sepsis and meningitis. The first step towards infection is colonization of the nasopharynx. Recently, it was shown that agglutinating antibodies play an important role in the prevention of mucosal colonization with S. pneumoniae. Here, we present a novel method to quantify antibody-dependent pneumococcal agglutination in a high-throughput manner using flow cytometry. We found that the concentration of agglutinating antibodies against pneumococcal capsule are directly correlated with changes in the size and complexity of bacterial aggregates, as measured by flow cytometry and confirmed by light microscopy. Using the increase in size, we determined the agglutination index. The cutoff value was set by measuring a series of non-agglutinating antibodies. With this method, we show that not only anti-polysaccharide capsule antibodies are able to induce agglutination but that also anti-PspA protein antibodies have agglutinating capabilities. In conclusion, we have described and validated a novel method to quantify pneumococcal agglutination, which can be used to screen sera from murine or human vaccination studies, in a high-throughput manner. PMID:28288168

  9. An automated analysis of highly complex flow cytometry-based proteomic data.

    PubMed

    Stuchlý, Jan; Kanderová, Veronika; Fišer, Karel; Cerná, Daniela; Holm, Anders; Wu, Weiwei; Hrušák, Ondřej; Lund-Johansen, Fridtjof; Kalina, Tomáš

    2012-02-01

    The combination of color-coded microspheres as carriers and flow cytometry as a detection platform provides new opportunities for multiplexed measurement of biomolecules. Here, we developed a software tool capable of automated gating of color-coded microspheres, automatic extraction of statistics from all subsets and validation, normalization, and cross-sample analysis. The approach presented in this article enabled us to harness the power of high-content cellular proteomics. In size exclusion chromatography-resolved microsphere-based affinity proteomics (Size-MAP), antibody-coupled microspheres are used to measure biotinylated proteins that have been separated by size exclusion chromatography. The captured proteins are labeled with streptavidin phycoerythrin and detected by multicolor flow cytometry. When the results from multiple size exclusion chromatography fractions are combined, binding is detected as discrete reactivity peaks (entities). The information obtained might be approximated to a multiplexed western blot. We used a microsphere set with >1,000 subsets, presenting an approach to extract biologically relevant information. The R-project environment was used to sequentially recognize subsets in two-dimensional space and gate them. The aim was to extract the median streptavidin phycoerythrin fluorescence intensity for all 1,000+ microsphere subsets from a series of 96 measured samples. The resulting text files were subjected to algorithms that identified entities across the 24 fractions. Thus, the original 24 data points for each antibody were compressed to 1-4 integrated values representing the areas of individual antibody reactivity peaks. Finally, we provide experimental data on cellular protein changes induced by treatment of leukemia cells with imatinib mesylate. The approach presented here exemplifies how large-scale flow cytometry data analysis can be efficiently processed to employ flow cytometry as a high-content proteomics method.

  10. A flow cytometry based oligotrophic pollutant exposure test to detect bacterial growth inhibition and cell injury.

    PubMed

    Czechowska, Kamila; van der Meer, Jan Roelof

    2011-07-01

    Toxicity of chemical pollutants in aquatic environments is often addressed by assays that inquire reproductive inhibition of test microorganisms, such as algae or bacteria. Those tests, however, assess growth of populations as a whole via macroscopic methods such as culture turbidity or colony-forming units. Here we use flow cytometry to interrogate the fate of individual cells in low-density populations of the bacterium Pseudomonas fluorescens SV3 exposed or not under oligotrophic conditions to a number of common pollutants, some of which derive from oil contamination. Cells were stained at regular time intervals during the exposure assay with fluorescent dyes that detect membrane injury (i.e., live-dead assay). Reduction of population growth rates was observed upon toxicant insult and depended on the type of toxicant. Modeling and cell staining indicate that population growth rate decrease is a combined effect of an increased number of injured cells that may or may not multiply, and live cells dividing at normal growth rates. The oligotrophic assay concept presented here could be a useful complement for existing biomarker assays in compliance with new regulations on chemical effect studies or, more specifically, for judging recovery after exposure to fluctuating toxicant conditions.

  11. Flow cytometry-based isolation of dermal lymphatic endothelial cells from newborn rats.

    PubMed

    Thiele, W; Rothley, M; Schmaus, A; Plaumann, D; Sleeman, J

    2014-12-01

    The lymphatic system plays a key role in tissue homeostasis, fatty acid transport, and immune surveillance. Pathologically, dysfunction of the lymphatic system results in edema, and increased lymphangiogenesis can contribute to tumor metastasis. Lymphatic vessels are composed of lymphatic endothelial cells (LECs) that can be identified by distinct marker molecules such as Prox-1, podoplanin, VEGFR-3 and LYVE-1. Primary LECs represent a valuable tool for the study of basic functions of the lymphatic system. However, their isolation remains a challenge, particularly if rodent tissues are used as a source. We developed a method for the isolation of rat dermal LECs from the skin of newborn rats based on sequential enzymatic digestion with trypsin and Liberase followed by flow cytometric sorting using LYVE-1 specific antibodies. Cells isolated according to this protocol expressed the lymphatic markers Prox-1, podoplanin, LYVE-1 and VEGFR-3, and displayed an endothelial-like morphology when taken into culture. These primary cells can be used for studying lymphatic biology in rat models, and the protocol we describe here therefore represents an important extension of the experimental repertoire available for rats and for modeling the human lymphatic system.

  12. Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria.

    PubMed

    Correia, Rodolfo Patussi; Bento, Laiz Cameirão; Bortolucci, Ana Carolina Apelle; Alexandre, Anderson Marega; Vaz, Andressa da Costa; Schimidell, Daniela; Pedro, Eduardo de Carvalho; Perin, Fabricio Simões; Nozawa, Sonia Tsukasa; Mendes, Cláudio Ernesto Albers; Barroso, Rodrigo de Souza; Bacal, Nydia Strachman

    2016-01-01

    To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones. Discutir as melhorias técnicas no diagnóstico e no acompanhamento laboratorial de hemoglobinúria paroxística noturna para a validação da técnica de citometria de fluxo de alta sensibilidade. Estudo retrospectivo, que envolveu a análise de dados laboratoriais de 745 pacientes com hipótese diagnóstica e/ou acompanhamento de hemoglobinúria paroxística noturna por citometria de fluxo. Os avanços técnicos não só reduziram o custo do ensaio, mas também melhoraram a identificação e a resolução da citometria de fluxo para a detecção de clone hemoglobinúria paroxística noturna. A citometria de fluxo de alta sensibilidade possibilitou a identificação do tipo e do tamanho de clone de hemoglobinúria paroxística noturna, especialmente em amostras com pequeno clone.

  13. Flow cytometry-based platelet function testing is predictive of symptom burden in a cohort of bleeders.

    PubMed

    Boknäs, Niklas; Ramström, Sofia; Faxälv, Lars; Lindahl, Tomas L

    2017-09-12

    Platelet function disorders (PFDs) are common in patients with mild bleeding disorders (MBDs), yet the significance of laboratory findings suggestive of a PFD remain unclear due to the lack of evidence for a clinical correlation between the test results and the patient phenotype. Herein, we present the results from a study evaluating the potential utility of platelet function testing using whole-blood flow cytometry in a cohort of 105 patients undergoing investigation for MBD. Subjects were evaluated with a test panel comprising two different activation markers (fibrinogen binding and P-selectin exposure) and four physiologically relevant platelet agonists (ADP, PAR1-AP, PAR4-AP, and CRP-XL). Abnormal test results were identified by comparison with reference ranges constructed from 24 healthy controls or with the fifth percentile of the entire patient cohort. We found that the abnormal test results are predictive of bleeding symptom severity, and that the greatest predictive strength was achieved using a subset of the panel, comparing measurements of fibrinogen binding after activation with all four agonists with the fifth percentile of the patient cohort (p = 0.00008, hazard ratio 8.7; 95% CI 2.5-40). Our results suggest that whole-blood flow cytometry-based platelet function testing could become a feasible alternative for the investigation of MBDs. We also show that platelet function testing using whole-blood flow cytometry could provide a clinically relevant quantitative assessment of platelet-related hemostasis.

  14. Tips and tricks for flow cytometry-based analysis and counting of microparticles.

    PubMed

    Poncelet, Philippe; Robert, Stéphane; Bailly, Nicolas; Garnache-Ottou, Francine; Bouriche, Tarik; Devalet, Bérangère; Segatchian, Jerard H; Saas, Philippe; Mullier, François

    2015-10-01

    Submicron-sized extra-cellular vesicles generated by budding from the external cell membranes, microparticles (MPs) are important actors in transfusion as well as in other medical specialties. After briefly positioning their role in the characterization of labile blood products, this technically oriented chapter aims to review practical points that need to be considered when trying to use flow cytometry for the analysis, characterization and absolute counting of MP subsets. Subjects of active discussions relative to instrumentation will include the choice of the trigger parameter, possible standardization approaches requiring instrument quality-control, origin and control of non-specific background and of coincidence artifacts, choice of the type of electronic signals, optimal sheath fluid and sample speed. Questions related to reagents will cover target antigens and receptors, multi-color reagents, negative controls, enumeration of MPs and limiting artifacts due to unexpected (micro-) coagulation of plasma samples. Newly detected problems are generating innovative solutions and flow cytometry will continue to remain the technology of choice for the analysis of MPs, in the domain of transfusion as well as in many diverse specialties. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Immune Monitoring in Cancer Vaccine Clinical Trials: Critical Issues of Functional Flow Cytometry-Based Assays

    PubMed Central

    Urbani, Francesca; Proietti, Enrico

    2013-01-01

    The development of immune monitoring assays is essential to determine the immune responses against tumor-specific antigens (TSAs) and tumor-associated antigens (TAAs) and their possible correlation with clinical outcome in cancer patients receiving immunotherapies. Despite the wide range of techniques used, to date these assays have not shown consistent results among clinical trials and failed to define surrogate markers of clinical efficacy to antitumor vaccines. Multiparameter flow cytometry- (FCM-) based assays combining different phenotypic and functional markers have been developed in the past decade for informative and longitudinal analysis of polyfunctional T-cells. These technologies were designed to address the complexity and functional heterogeneity of cancer biology and cellular immunity and to define biomarkers predicting clinical response to anticancer treatment. So far, there is still a lack of standardization of some of these immunological tests. The aim of this review is to overview the latest technologies for immune monitoring and to highlight critical steps involved in some of the FCM-based cellular immune assays. In particular, our laboratory is focused on melanoma vaccine research and thus our main goal was the validation of a functional multiparameter test (FMT) combining different functional and lineage markers to be applied in clinical trials involving patients with melanoma. PMID:24195078

  16. Automated sperm concentration analysis with a new flow cytometry-based device, S-FCM.

    PubMed

    Tsuji, Takumi; Okada, Hiroshi; Fujisawa, Masato; Hamaguchi, Yukio; Kamidono, Sadao

    2002-03-01

    The S-FCM uses flow cytometry technology to measure sperm concentrations. Semen samples from 104 men attending a male infertility clinic were used to evaluate the reproducibility of results and the carryover rate with the S-FCM by performing between- and within-run imprecision analyses. In addition, sperm concentrations measured with the S-FCM were compared with those obtained by manual analyses with the Makler chamber and the improved Neubauer hemacytometer. The results showed that automated analyses with the S-FCM were highly reproducible and the carryover rate was 0.17% or less. In within-run imprecision assays, the coefficients of variation for the S-FCM were less than 5% at all sperm concentrations, while those for the Makler chamber were between 17.7% and 28.7% at lower sperm concentrations. The overall correlation between values measured with the S-FCM and those measured with the Makler chamber and improved Neubauer hemacytometer was excellent, but at lower sperm concentrations the correlation was lower. The S-FCM performed sperm concentration analyses in 110 seconds compared with 5 minutes for the Makler chamber and 10 minutes for the improved Neubauer hemacytometer. The S-FCM is suitable for quantitative measurement of lower sperm concentrations.

  17. Purification of Immune Cell Populations from Freshly Isolated Murine Tumors and Organs by Consecutive Magnetic Cell Sorting and Multi-parameter Flow Cytometry-Based Sorting.

    PubMed

    Salvagno, Camilla; de Visser, Karin E

    2016-01-01

    It is well established that tumors evolve together with nonmalignant cells, such as fibroblasts, endothelial cells, and immune cells. These cells constantly entangle and interact with each other creating the tumor microenvironment. Immune cells can exert both tumor-promoting and tumor-protective functions. Detailed phenotypic and functional characterization of intra-tumoral immune cell subsets has become increasingly important in the field of cancer biology and cancer immunology. In this chapter, we describe a method for isolation of viable and pure immune cell subsets from freshly isolated murine solid tumors and organs. First, we describe a protocol for the generation of single-cell suspensions from tumors and organs using mechanical and enzymatic strategies. In addition, we describe how immune cell subsets can be purified by consecutive magnetic cell sorting and multi-parameter flow cytometry-based cell sorting.

  18. Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis.

    PubMed

    Silva-dos-Santos, Priscila Pinto; Barros, Geisa Baptista; Mineo, José Roberto; de Oliveira Silva, Deise Aparecida; Menegaz, Mauro Hygino Weinert; Serufo, José Carlos; Dietze, Reynaldo; Martins-Filho, Olindo de Assis; Lemos, Elenice Moreira

    2012-04-30

    In the present study we evaluated the performance of a flow cytometry-based algorithm as a new serological approach to detect antibodies to T. gondii and specific IgG avidity to diagnose acute toxoplasmosis. The results showed that using FC-AFTA-IgM assay, all serum samples from patients with acute toxoplasmosis demonstrated seropositivity, whereas 90% of patients with chronic infection and 100% of non-infected individuals presented negative results. Thus, only 10% of patients with chronic toxoplasmosis showed residual IgM, in contrast with other methodologies used to diagnosis acute toxoplasmosis. On the order hand, FC-AFTA-IgG assay as well as FC-AFTA-IgG subclasses is unlikely to discriminate acute from chronic toxoplasmosis. We have also evaluated the performance of FC-AFTA-IgG avidity as a tool to exclude chronic toxoplasmosis in patients with positive FC-AFTA-IgM. Our data showed an excellent performance of FC-AFTA-IgG avidity employing the cut-off of 60% for Avidity Index (AI) with sensitivity and specificity of 100%. All serum samples from patients presenting acute toxoplasmosis showed low avidity index (AI≤60%), whereas all chronic patients showed high avidity index (AI>60%). The outstanding performance indexes of this novel flow cytometry-based algorithm support its use as a non-conventional alternative serological approach to diagnose human acute toxoplasmosis.

  19. A flow cytometry-based immuno-titration assay for rapid and accurate titer determination of modified vaccinia Ankara virus vectors.

    PubMed

    Li, Zengji; Ling, Loni; Liu, Xiaohui; Laus, Reiner; Delcayre, Alain

    2010-10-01

    A flow cytometry-based immuno-titration titer assay was established to determine infectious unit (IU) and transducing unit (TU) of modified vaccinia Ankara (MVA) virus vectors. This titration method enumerates infected cells by measuring the expression of viral protein for IU and transgene protein for TU in individual cells after staining with fluorophore-conjugated antibodies. It presents many advantages over standard virus titration approaches, such as TCID(50) or plaque assay, for its convenience, rapidity and accuracy as illustrated by excellent assay linearity and reproducibility. Importantly, the IU and the TCID(50) assays generated similar batch-specific titer values when testing varied MVA-derived virus preparations. Assay development revealed that the post-infection time at which viral protein expression is evaluated, host cell type, and blocking the formation and release of progeny virion with nocodazole, an anti-microtubule agent or rifampin, a specific vaccinia virus assembly inhibitor, are critical parameters for the precision, robustness, and accuracy of IU titer determination. An added advantage of this assay is that it enables the concurrent determination of IU and transducing units (TU) by measuring the expression of a transgene product when testing recombinant viruses. The latter was demonstrated using a MVA vector carrying a human HER-2 gene fragment as model. Hence, this assay is very versatile in that it can be used to determine IU as well as multiple TU titers simultaneously. Furthermore, it can readily be adapted to other poxvirus vectors. Copyright (c) 2010 Elsevier B.V. All rights reserved.

  20. A flow cytometry-based workflow for detection and quantification of anti-plasmodial antibodies in vaccinated and naturally exposed individuals

    PubMed Central

    2012-01-01

    Background Antibodies play a central role in naturally acquired immunity against Plasmodium falciparum. Current assays to detect anti-plasmodial antibodies against native antigens within their cellular context are prone to bias and cannot be automated, although they provide important information about natural exposure and vaccine immunogenicity. A novel, cytometry-based workflow for quantitative detection of anti-plasmodial antibodies in human serum is presented. Methods Fixed red blood cells (RBCs), infected with late stages of P. falciparum were utilized to detect malaria-specific antibodies by flow cytometry with subsequent automated data analysis. Available methods for data-driven analysis of cytometry data were assessed and a new overlap subtraction algorithm (OSA) based on open source software was developed. The complete workflow was evaluated using sera from two GMZ2 malaria vaccine trials in semi-immune adults and pre-school children residing in a malaria endemic area. Results Fixation, permeabilization, and staining of infected RBCs were adapted for best operation in flow cytometry. As asexual blood-stage vaccine candidates are designed to induce antibody patterns similar to those in semi-immune adults, serial dilutions of sera from heavily exposed individuals were compared to naïve controls to determine optimal antibody dilutions. To eliminate investigator effects introduced by manual gating, a non-biased algorithm (OSA) for data-driven gating was developed. OSA-derived results correlated well with those obtained by manual gating (r between 0.79 and 0.99) and outperformed other model-driven gating methods. Bland-Altman plots confirmed the agreement of manual gating and OSA-derived results. A 1.33-fold increase (p=0.003) in the number of positive cells after vaccination in a subgroup of pre-school children vaccinated with 100 μg GMZ2 was present and in vaccinated adults from the same region we measured a baseline-corrected 1.23-fold, vaccine

  1. Flow cytometry-based analysis of artemisinin-resistant Plasmodium falciparum in the ring-stage survival assay.

    PubMed

    Amaratunga, Chanaki; Neal, Aaron T; Fairhurst, Rick M

    2014-08-01

    The ring-stage survival assay (RSA) is a powerful tool for phenotyping artemisinin-resistant Plasmodium falciparum but requires experienced microscopists to count viable parasites among 10,000 erythrocytes in Giemsa-stained thin blood smears. Here we describe a rapid flow cytometric assay that accurately counts viable parasites among 250,000 erythrocytes in suspension. This method performs as well as light microscopy and can be used to standardize the collection of RSA data between research groups in laboratory and field settings.

  2. Development of Leishmania donovani stably expressing DsRed for flow cytometry-based drug screening using chalcone thiazolyl-hydrazone as a new antileishmanial target.

    PubMed

    Jaiswal, Anil Kumar; Rao, K Bhaskara; Kushwaha, Pragati; Rawat, Keerti; Modukuri, Ram K; Khare, Prashant; Joshi, Sumit; Mishra, Shikha; Rai, Ambak; Sashidhara, Koneni V; Dube, Anuradha

    2016-12-01

    Green fluorescent protein produces significant fluorescence and is extremely stable, however its excitation maximum is close to the ultraviolet range and thus can damage living cells. Hence, Leishmania donovani stably expressing DsRed were developed and their suitability for flow cytometry-based antileishmanial screening was assessed by evaluating the efficacies of standard drugs as well as newly synthesised chalcone thiazolyl-hydrazone compounds. The DsRed gene was successfully integrated at the 18S rRNA locus of L. donovani and transfectants (LdDsRed) were selected using hygromycin B. Enhanced expression of DsRed and a high level of infectivity to J774A.1 macrophages were achieved, which was confirmed by fluorescence microscopy and flow cytometry. Furthermore, these LdDsRed transfectants were utilised for development of an in vitro screening assay using the standard antileishmanial drugs miltefosine, amphotericin B, pentamidine and paromomycin. The response of transfectants to standard drugs correlated well with previous reports. Subsequently, the suitability of this system was further assessed by screening a series of 18 newly synthesised chalcone thiazolyl-hydrazone compounds in vitro for their antileishmanial activity, wherein 8 compounds showed moderate antileishmanial activity. The most active compound 5g, with ca. 73% splenic parasite reduction, exerted its activity via generating nitric oxide and reactive oxygen species and inducing apoptosis in LdDsRed-infected macrophages. Thus, these observations established the applicability of LdDsRed transfectants for flow cytometry-based antileishmanial screening. Further efforts aimed at establishing a high-throughput screening assay and determining the in vivo screening of potential antileishmanial leads are required.

  3. An imaging flow cytometry-based approach to measuring the spatiotemporal calcium mobilisation in activated T cells.

    PubMed

    Cerveira, Joana; Begum, Julfa; Di Marco Barros, Rafael; van der Veen, Annemarthe G; Filby, Andrew

    2015-08-01

    Calcium ions (Ca(2+)) are a ubiquitous transducer of cellular signals controlling key processes such as proliferation, differentiation, secretion and metabolism. In the context of T cells, stimulation through the T cell receptor has been shown to induce the release of Ca(2+) from intracellular stores. This sudden elevation within the cytoplasm triggers the opening of ion channels in the plasma membrane allowing an influx of extracellular Ca(2+) that in turn activates key molecules such as calcineurin. This cascade ultimately results in gene transcription and changes in the cellular state. Traditional methods for measuring Ca(2+) include spectrophotometry, conventional flow cytometry (CFC) and live cell imaging techniques. While each method has strengths and weaknesses, none can offer a detailed picture of Ca(2+) mobilisation in response to various agonists. Here we report an Imaging Flow Cytometry (IFC)-based method that combines the throughput and statistical rigour of CFC with the spatial information of a microscope. By co-staining cells with Ca(2+) indicators and organelle-specific dyes we can address the spatiotemporal patterns of Ca(2+) flux in Jurkat cells after stimulation with anti-CD3. The multispectral, high-throughput nature of IFC means that the organelle co-staining functions to direct the measurement of Ca(2+) indicator fluorescence to either the endoplasmic reticulum (ER) or the mitochondrial compartments without the need to treat cells with detergents such as digitonin to eliminate cytoplasmic background. We have used this system to look at the cellular localisation of Ca(2+) after stimulating cells with CD3, thapsigargin or ionomycin in the presence or absence of extracellular Ca(2+). Our data suggest that there is a dynamic interplay between the ER and mitochondrial compartments and that mitochondria act as a sink for both intracellular and extracellular derived Ca(2+). Moreover, by generating an NFAT-GFP expressing Jurkat line, we were able to

  4. A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer

    PubMed Central

    Pallinger, Eva; Bognar, Zoltan; Bodis, Jozsef; Csabai, Timea; Farkas, Nelli; Godony, Krisztina; Varnagy, Akos; Buzas, Edit; Szekeres-Bartho, Julia

    2017-01-01

    Multiple pregnancy is a risk for prematurity and preterm birth. The goal of assisted reproduction is to achieve a single pregnancy, by transferring a single embryo. This requires improved methods to identify the competent embryo. Here, we describe such a test, based on flow cytometric determination of the nucleic acid (PI+) containing extracellular vesicle (EV) count in day 5 embryo culture media. 88 women undergoing IVF were included in the study. More than 1 embryos were transferred to most patients. In 58 women, the transfer resulted in clinical pregnancy, whereas in 30 women in implantation failure. In 112 culture media of embryos from the “clinical pregnancy” group, the number of PI+ EVs was significantly lower than in those of 49 embryos, from the “implantation failure” group. In 14 women, transfer of a single embryo resulted in a singleton pregnancy, or, transfer of two embryos in twin pregnancy. The culture media of 19 out of the 20 “confirmed competent” embryos contained a lower level of PI+ EVs than the cut off level, suggesting that the competent embryo can indeed be identified by low PI+ EV counts. We developed a noninvasive, simple, inexpensive, quick test, which identifies the embryos that are most likely to implant. PMID:28057937

  5. An Imaging Flow Cytometry-based approach to analyse the fission yeast cell cycle in fixed cells.

    PubMed

    Patterson, James O; Swaffer, Matthew; Filby, Andrew

    2015-07-01

    Fission yeast (Schizosaccharomyces pombe) is an excellent model organism for studying eukaryotic cell division because many of the underlying principles and key regulators of cell cycle biology are conserved from yeast to humans. As such it can be employed as tool for understanding complex human diseases that arise from dis-regulation in cell cycle controls, including cancers. Conventional Flow Cytometry (CFC) is a high-throughput, multi-parameter, fluorescence-based single cell analysis technology. It is widely used for studying the mammalian cell cycle both in the context of the normal and disease states by measuring changes in DNA content during the transition through G1, S and G2/M using fluorescent DNA-binding dyes. Unfortunately analysis of the fission yeast cell cycle by CFC is not straightforward because, unlike mammalian cells, cytokinesis occurs after S-phase meaning that bi-nucleated G1 cells have the same DNA content as mono-nucleated G2 cells and cannot be distinguished using total integrated fluorescence (pulse area). It has been elegantly shown that the width of the DNA pulse can be used to distinguish G2 cells with a single 2C foci versus G1 cells with two 1C foci, however the accuracy of this measurement is dependent on the orientation of the cell as it traverses the laser beam. To this end we sought to improve the accuracy of the fission yeast cell cycle analysis and have developed an Imaging Flow Cytometry (IFC)-based method that is able to preserve the high throughput, objective analysis afforded by CFC in combination with the spatial and morphometric information provide by microscopy. We have been able to derive an analysis framework for subdividing the yeast cell cycle that is based on intensiometric and morphometric measurements and is thus robust against orientation-based miss-classification. In addition we can employ image-based metrics to define populations of septated/bi-nucleated cells and measure cellular dimensions. To our knowledge

  6. A new flow-cytometry-based opsonophagocytosis assay for the rapid measurement of functional antibody levels against Group B Streptococcus.

    PubMed

    Fabbrini, Monica; Sammicheli, Chiara; Margarit, Immaculada; Maione, Domenico; Grandi, Guido; Giuliani, Marzia Monica; Mori, Elena; Nuti, Sandra

    2012-04-30

    Opsonophagocytosis is the primary mechanism for the clearance of Group B Streptococcus (GBS) by the host, and levels of opsonic antibodies may correlate with protection in preclinical models. A killing-based opsonophagocytosis assay (OPA), can be used to determine the functional activity of vaccine-induced GBS-specific antibodies. The assay, which measures the number of bacterial colonies surviving phagocytic killing in the presence of specific antibodies and complement, is rather expensive, time-consuming and poorly standardized. Here we describe a rapid, sensitive and reproducible fluorescent OPA assay (fOPA) based on flow cytometry analysis (FACS), which allows internalized bacteria to be distinguished from those associated to the plasma membrane of phagocytic cells. Fixed GBS were labeled with pHrodo™, a fluorescent dye which dramatically increases the emitted fluorescence at the acidic conditions present in the phagocytic endosomal compartment. Labeled bacteria were incubated with HL-60 cells differentiated to phagocytes, antibodies and complement, and then analyzed by FACS. A further improvement to our method, allowing to reduce assay variability, consisted on a step of selection of effector cells among the HL-60 population. Analysis of sera from mice immunized with different GBS vaccines revealed comparable sensitivity and specificity with the traditional killing OPA assay (kOPA), and a good correlation between the fluorescent signal of bacteria internalized by HL-60 phagocytes and killing. Remarkably, the pHrodo-based approach reduced the variability observed with other fOPA assays. The obtained data indicate the proposed fOPA as a reliable and useful tool for functional antibody assessment.

  7. Detection of Vibrio cholerae O1 and O139 in environmental waters of rural Bangladesh: a flow-cytometry-based field trial.

    PubMed

    Righetto, L; Zaman, R U; Mahmud, Z H; Bertuzzo, E; Mari, L; Casagrandi, R; Gatto, M; Islam, S; Rinaldo, A

    2015-08-01

    Presence of Vibrio cholerae serogroups O1 and O139 in the waters of the rural area of Matlab, Bangladesh, was investigated with quantitative measurements performed with a portable flow cytometer. The relevance of this work relates to the testing of a field-adapted measurement protocol that might prove useful for cholera epidemic surveillance and for validation of mathematical models. Water samples were collected from different water bodies that constitute the hydrological system of the region, a well-known endemic area for cholera. Water was retrieved from ponds, river waters, and irrigation canals during an inter-epidemic time period. Each sample was filtered and analysed with a flow cytometer for a fast determination of V. cholerae cells contained in those environments. More specifically, samples were treated with O1- and O139-specific antibodies, which allowed precise flow-cytometry-based concentration measurements. Both serogroups were present in the environmental waters with a consistent dominance of V. cholerae O1. These results extend earlier studies where V. cholerae O1 and O139 were mostly detected during times of cholera epidemics using standard culturing techniques. Furthermore, our results confirm that an important fraction of the ponds' host populations of V. cholerae are able to self-sustain even when cholera cases are scarce. Those contaminated ponds may constitute a natural reservoir for cholera endemicity in the Matlab region. Correlations of V. cholerae concentrations with environmental factors and the spatial distribution of V. cholerae populations are also discussed.

  8. Evaluation of T and B lymphocyte function in clinical practice using a flow cytometry based proliferation assay.

    PubMed

    Marits, Per; Wikström, Ann-Charlotte; Popadic, Dusan; Winqvist, Ola; Thunberg, Sarah

    2014-08-01

    The golden standard for functional evaluation of immunodeficiencies is the incorporation of [(3)H]-thymidine in a proliferation assay stimulated with mitogens. Recently developed whole blood proliferation assays have the advantage of parallel lymphocyte lineage analysis and in addition provide a non-radioactive alternative. Here we evaluate the Flow-cytometric Assay for Specific Cell-mediated Immune-response in Activated whole blood (FASCIA) in a comparison with [(3)H]-thymidine incorporation in four patients with severe combined immunodeficiency. The threshold for the minimum number of lymphocytes required for reliable responses in FASCIA is determined together with reference values from 100 healthy donors when stimulated with mitogens as well as antigen specific stimuli. Finally, responses against PWM and SEA+SEB stimuli are conducted with clinically relevant immunomodulatory compounds. We conclude that FASCIA is a rapid, stable and sensitive functional whole blood assay that requires small amounts of whole blood that can be used for reliable assessment of lymphocyte reactivity in patients.

  9. An approach for plasma cell myeloma diagnosis by two-color flow cytometry based on kappa/lambda ratios of CD38-gated plasma cells.

    PubMed

    Nakayama, S; Yokote, T; Hirata, Y; Iwaki, K; Akioka, T; Miyoshi, T; Takayama, A; Nishiwaki, U; Masuda, Y; Ikemoto, T; Tanaka, H; Nishimura, Y; Tsuji, M; Hanafusa, T

    2013-01-01

    Criteria from the World Health Organization (WHO) are commonly used to diagnose plasma cell myeloma (PCM), but they are complex and require several laboratory parameters. To differentiate reactive plasmacytosis from clonal plasma cell neoplasms, such as PCM, it is important to accurately determine the expression of the cytoplasmic immunoglobulin (cIg) light chain (LC). Through retrospective analyses, we selected the patients with PCM, and analyzed records of 52 PCM patients, who underwent bone biopsies, and final diagnosis of PCM was established according to WHO criteria, and 22 controls. In the present study, all samples were analyzed by flow cytometry (FC) in the side scatter vs CD38 histogram mode, and the CD38-gated plasma cell population was identified. The positive cell ratios of kappa and lambda to plasma cell populations were analyzed. PCM cells were distinguished from normal plasma cells by a cut-off level between 0.80 and 3.3, a sensitivity of 90.3 percent, and a specificity of 81.1 percent. Two-color FC analysis is simple to perform, inexpensive, and clinically relevant data are obtained soon after completion of the FC measurements. It could be one of the helpful tools in the diagnosis of PCM. The correct diagnosis of PCM can be achieved more simply, efficiently, and rapidly by combining this method.

  10. An approach for diagnosing plasma cell myeloma by three-color flow cytometry based on kappa/lambda ratios of CD38-gated CD138(+) cells.

    PubMed

    Nakayama, Shoko; Yokote, Taiji; Hirata, Yuji; Iwaki, Kazuki; Akioka, Toshikazu; Miyoshi, Takuji; Takayama, Ayami; Nishiwaki, Uta; Masuda, Yuki; Ikemoto, Toshiyuki; Tanaka, Hidema; Nishimura, Yasuichiro; Tsuji, Motomu; Hanafusa, Toshiaki

    2012-09-28

    World Health Organization (WHO) criteria are commonly used to diagnose plasma cell myeloma (PCM); however, these criteria are complex and require several laboratory parameters. For differentiating reactive plasmacytosis from clonal plasma cell (PC) neoplasms such as PCM, it is important to accurately determine the expression of cytoplasmic immunoglobulin light chains. We retrospectively analyzed the records of 27 selected patients with PCM who underwent bone biopsies for confirmative diagnosis according to WHO criteria. Twenty-three controls were also investigated. In the present study, all the samples were analyzed using flow cytometry (FC) in the side scatter vs. CD38 histogram mode, and the CD38-gated PC population was identified. Bivariate histograms of CD138/kappa and CD138/lambda were assessed, and the ratios of dual-positive cells to the CD138(+) PC population were calculated. The kappa/lambda ratio was defined as the ratio of CD138/kappa to CD138/lambda. PCM cells were distinguished from normal PCs using cutoff levels between 0.76 and 1.5, at a sensitivity of 96.3% and specificity of 95.7%. Three-color FC analysis is simple to perform and inexpensive, with clinically relevant data obtained soon after the completion of FC measurements. The detection of the cytoplasmic kappa/lambda ratio of CD38-gated CD138(+) PCs may be a useful tool in the diagnosis of PCM. To the best of our knowledge, this report represents the first diagnostic assessment of the cytoplasmic kappa/lambda ratio in CD38-gated CD138+ PCs using FC analysis. This method may help in more simple, efficient, rapid, and accurate diagnosis of PCM. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1568085959771735.

  11. Multiplex immunoassay for persistent organic pollutants in tilapia: Comparison of imaging- and flow cytometry-based platforms using spectrally encoded paramagnetic microspheres

    USDA-ARS?s Scientific Manuscript database

    Recent developments in spectrally encoded microspheres (SEMs)-based technologies provide high multiplexing possibilities. Most SEMs-based assays required a flow cytometer with sophisticated fluidics and optics. The new imaging superparamagnetic SEMs-based platform transports SEMs with considerably ...

  12. In vivo imaging flow cytometry based on laser scanning two-photon microscopy at kHz cross-sectional frame rate

    NASA Astrophysics Data System (ADS)

    Kong, Lingjie; Tang, Jianyong; Cui, Meng

    2016-03-01

    In vivo flow cytometry has found numerous applications in biology and pharmacology. However, conventional cytometry does not provide the detailed morphological information that is needed to fully determine the phenotype of individual circulating cells. Imaging cytometry, capable of visualizing the morphology and dynamics of the circulating cells at high spatiotemporal resolution, is highly desired. Current wide-field based image cytometers are limited in the imaging depth and provide only two-dimensional resolution. For deep tissue imaging, laser scanning two-photon fluorescence microscopy (TPM) is widely adopted. However, for applications in flow cytometry, the axial scanning speed of current TPMs is inadequate to provide high-speed cross-sectional imaging of vasculature. We have integrated an optical phase-locked ultrasound lens into a standard TPM and achieved microsecond-scale axial scanning. With a galvo scanner for transverse scanning, we achieved kHz cross-sectional frame rate. Here we report its applications for in vivo deformability cytometry and in vivo imaging flow cytometry, and demonstrate the capability of imaging dynamical morphologies of flowing cells, distinguishing cells and cellular clusters, and simultaneously quantifying different cell populations based on their fluorescent labels.

  13. A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4/Tmem53 as involved in stress-dependent cell cycle withdrawal.

    PubMed

    Korfali, Nadia; Srsen, Vlastimil; Waterfall, Martin; Batrakou, Dzmitry G; Pekovic, Vanja; Hutchison, Christopher J; Schirmer, Eric C

    2011-04-14

    Disruption of cell cycle regulation is one mechanism proposed for how nuclear envelope protein mutation can cause disease. Thus far only a few nuclear envelope proteins have been tested/found to affect cell cycle progression: to identify others, 39 novel nuclear envelope transmembrane proteins were screened for their ability to alter flow cytometry cell cycle/DNA content profiles when exogenously expressed. Eight had notable effects with seven increasing and one decreasing the 4N:2N ratio. We subsequently focused on NET4/Tmem53 that lost its effects in p53(-/-) cells and retinoblastoma protein-deficient cells. NET4/TMEM53 knockdown by siRNA altered flow cytometry cell cycle/DNA content profiles in a similar way as overexpression. NET4/TMEM53 knockdown did not affect total retinoblastoma protein levels, unlike nuclear envelope-associated proteins Lamin A and LAP2α. However, a decrease in phosphorylated retinoblastoma protein was observed along with a doubling of p53 levels and a 7-fold increase in p21. Consequently cells withdrew from the cell cycle, which was confirmed in MRC5 cells by a drop in the percentage of cells expressing Ki-67 antigen and an increase in the number of cells stained for ß-galactosidase. The ß-galactosidase upregulation suggests that cells become prematurely senescent. Finally, the changes in retinoblastoma protein, p53, and p21 resulting from loss of NET4/Tmem53 were dependent upon active p38 MAP kinase. The finding that roughly a fifth of nuclear envelope transmembrane proteins screened yielded alterations in flow cytometry cell cycle/DNA content profiles suggests a much greater influence of the nuclear envelope on the cell cycle than is widely held.

  14. A Flow Cytometry-Based Screen of Nuclear Envelope Transmembrane Proteins Identifies NET4/Tmem53 as Involved in Stress-Dependent Cell Cycle Withdrawal

    PubMed Central

    Waterfall, Martin; Batrakou, Dzmitry G.; Pekovic, Vanja; Hutchison, Christopher J.; Schirmer, Eric C.

    2011-01-01

    Disruption of cell cycle regulation is one mechanism proposed for how nuclear envelope protein mutation can cause disease. Thus far only a few nuclear envelope proteins have been tested/found to affect cell cycle progression: to identify others, 39 novel nuclear envelope transmembrane proteins were screened for their ability to alter flow cytometry cell cycle/DNA content profiles when exogenously expressed. Eight had notable effects with seven increasing and one decreasing the 4N∶2N ratio. We subsequently focused on NET4/Tmem53 that lost its effects in p53−/− cells and retinoblastoma protein-deficient cells. NET4/TMEM53 knockdown by siRNA altered flow cytometry cell cycle/DNA content profiles in a similar way as overexpression. NET4/TMEM53 knockdown did not affect total retinoblastoma protein levels, unlike nuclear envelope-associated proteins Lamin A and LAP2α. However, a decrease in phosphorylated retinoblastoma protein was observed along with a doubling of p53 levels and a 7-fold increase in p21. Consequently cells withdrew from the cell cycle, which was confirmed in MRC5 cells by a drop in the percentage of cells expressing Ki-67 antigen and an increase in the number of cells stained for ß-galactosidase. The ß-galactosidase upregulation suggests that cells become prematurely senescent. Finally, the changes in retinoblastoma protein, p53, and p21 resulting from loss of NET4/Tmem53 were dependent upon active p38 MAP kinase. The finding that roughly a fifth of nuclear envelope transmembrane proteins screened yielded alterations in flow cytometry cell cycle/DNA content profiles suggests a much greater influence of the nuclear envelope on the cell cycle than is widely held. PMID:21533191

  15. The synthetic food colouring agent Allura Red AC (E129) is not genotoxic in a flow cytometry-based micronucleus assay in vivo.

    PubMed

    Abramsson-Zetterberg, Lilianne; Ilbäck, Nils-Gunnar

    2013-09-01

    The safety of several azo colouring agents, used as food additives, has during the years been questioned. Allura Red AC (E129) has in some publications been classified as genotoxic. In fact, in the European Union, Allura Red is permitted as a food additive in human food, but, surprisingly, it was not acceptable as an additive for use in animal feed. In this study we have evaluated whether Allura Red is genotoxic using a flow cytometer-based micronucleus assay in peripheral blood of mice. Male FVB mice were given a single intra-peritoneal injection of various doses of Allura Red and sacrificed at 46 h after treatment. The tested doses were 0, 100, 200, 400, 600, 800, 1000, 1500, and 2000 mg/kg body weight (b.w.). Each dose group constituted three mice, except for in the dose group of 1000 mg/kg b. w., which constituted four mice. Blood samples were collected and the frequency of micronucleated polychromatic erythrocytes (fMNPCE) and the cell proliferation (%PCE) was determined. The analyses did not show any significant difference in the %PCE or in the fMNPCE. Consequently, under the testing circumstances one can conclude that Allura Red is not genotoxic.

  16. Multiplex flow cytometry-based assay to study the breadth of antibody responses against E1E2 glycoproteins of hepatitis C virus.

    PubMed

    Merat, Sabrina J; van de Berg, Dorien; Bru, Camille; Yasuda, Etsuko; Breij, Esther; Koostra, Neeltje; Prins, Maria; Molenkamp, Richard; Bakker, Arjen Q; de Jong, Menno D; Spits, Hergen; Schinkel, Janke; Beaumont, Tim

    2017-08-27

    Hepatitis C virus (HCV) infection is a major global public health problem. In infected subjects who clear HCV infection, a strong antibody response has proven to be a correlate of protection. Understanding the antibody response in multiple subjects in large-scale studies would greatly benefit vaccine development. To determine the breadth of a polyclonal-serum antibody response, and or, the monoclonal antibodies against the different HCV E1E2 genotypes, we developed a quick and high throughput flow cytometry assay using fluorescent cell barcoding to distinguish cells transfected with different E1E2 sequences in a single measurement. HCV-specific antibodies recognizing conformational epitopes were tested for binding to cells transfected with E1E2 from six genotypes. In this assay, 1500 samples can be analyzed for specific binding to 6 different HCV E1E2 sequences within 8h. Plasma of chronically infected HCV subjects were tested in our assay allowing us to determine the breadth of their antibody response. In summary, we developed a quick and high throughput assay to study the specificity of an antibody response against multiple HCV E1E2 sequences simultaneously. This assay can also be used to facilitate the discovery of novel antibodies, and because other flavi- and picornaviruses have similar intracellular assembly mechanisms, this approach can be used to study the antibody response against such viruses. Copyright © 2017. Published by Elsevier B.V.

  17. Flow cytometry-based TCR-ligand Koff -rate assay for fast avidity screening of even very small antigen-specific T cell populations ex vivo.

    PubMed

    Nauerth, Magdalena; Stemberger, Christian; Mohr, Fabian; Weißbrich, Bianca; Schiemann, Matthias; Germeroth, Lothar; Busch, Dirk H

    2016-09-01

    High epitope-specific sensitivity of CD8(+) T cells is required for optimal immune protection against intracellular pathogens as well as certain malignancies. The quality of antigen recognition of CD8(+) T cells is usually described as "avidity" to its cognate peptide MHCI complex. T cell avidity is mainly dependent on the structural qualities of the T cell receptor (TCR), as convincingly demonstrated by recombinant TCR re-expression experiments. Based on reversible MHCI multimer staining and koff -rate measurements of monomeric peptide MHCI complexes, we recently established a microscopic assay for determining the structural avidity of individual CD8(+) T cells. Here we demonstrate that this assay can be adapted for rapid flow-cytometric avidity screening of epitope-specific T cell populations. Furthermore, we show that-in combination with conventional nonreversible MHCI multimer staining-even very small epitope-specific CD8(+) T cell populations can be analyzed directly ex vivo without the need for previous TCR cloning or T cell sorting. This simplified approach provides highly accurate mean TCR-ligand koff -rate values for poly- or oligoclonal T cell populations and is ideally suited for high-throughput applications in basic research as well as clinical settings. © 2016 International Society for Advancement of Cytometry.

  18. A novel flow cytometry-based cell capture platform for the detection, capture and molecular characterization of rare tumor cells in blood

    PubMed Central

    2014-01-01

    Background Personalized cancer treatment relies on the accurate detection of actionable genomic aberrations in tumor cells. Circulating tumor cells (CTCs) could provide an alternative genetic resource for diagnosis; however, the technical difficulties in isolating and analyzing rare CTCs have limited progress to date. In this preclinical study, we aimed to develop an improved capture system for molecular characterization of CTCs based on a novel cell sorting technology. Methods We developed a cell capture platform using On-chip Sort (On-Chip Biotechnologies), a novel bench-top cell sorter equipped with a disposable microfluidic chip. Spike-in experiments comprising a series of lung cancer cell lines with varying epithelial cell adhesion molecule (EpCAM) expression levels were conducted to assess the capture and purification efficiency of the platform. Samples were negatively enriched using anti-CD45-coated magnetic beads to remove white blood cells, followed by sample fixation and labeling. The enriched and labeled samples were then sorted by On-chip Sort based on cytokeratin, vimentin, and CD45 expression. Captured cells were immediately subjected to whole genome amplification followed by mutation analysis using deep targeted sequencing, and copy number analysis using quantitative polymerase chain reaction (qPCR). Results Spike-in experiments revealed an excellent overall mean capture rate of 70.9%. A 100% success rate in the detection of EGFR, KRAS and BRAF mutations from captured cells was achieved using pyrosequencing and deep sequencing. The mutant variant detection rates were markedly higher than those obtained with the CellSearch profile kit. qPCR analysis of amplified DNA demonstrated reproducible detection of copy number changes of the EGFR in captured tumor cells. Conclusions Using a novel cell sorter, we established an efficient and convenient platform for the capture of CTCs. Results of a proof-of-principle preclinical study indicated that this platform

  19. High degree of concordance between flow cytometry and geno2pheno methods for HIV-1 tropism determination in proviral DNA.

    PubMed

    Torres, Alex José Leite; Brígido, Luis Fernando de Macedo; Abrahão, Marcos Herculano Nunes; Angelo, Ana Luiza Dias; de Jesus Ferreira, Gilcivaldo; Coelho, Luana Portes; Ferreira, João Leandro; Jorge, Célia Regina Mayoral Pedroso; Netto, Eduardo Martins; Brites, Carlos

    2015-01-01

    Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a flow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identified by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identification of X4 and R5 tropic viruses was attempted by geno2pheno algorithm. There was a high degree of concordance between cytometry-based tropism assay and geno2pheno algorithm in determination of HIV-1 tropism. Cytometry-based tropism assay demonstrated higher sensitivity and specificity in comparison to geno2pheno, which was used as a gold-standard. One sample could not be amplified by geno2pheno method, but was classified as duotropic by cytometry-based tropism assay. We did not find any association between CD4+ count or plasma HIV-1 RNA viral load and tropism results. The overall performances of cytometry-based tropism assay and geno2pheno assay were almost identical in determination of HIV-1 tropism. Copyright © 2015 Elsevier Editora Ltda. All rights reserved.

  20. High-throughput vibrational cytometry based on nonlinear Raman microspectroscopy

    NASA Astrophysics Data System (ADS)

    Arora, R.; Petrov, G. I.; Yakovlev, V. V.

    2010-02-01

    Flow cytometry is a technology that allows a single cell or particle to be measured for a variety of characteristics, determined by looking at their properties while they flow in a liquid stream. High speed of flow and huge number of objects to be analyzed imposed some strict criteria on which methods can be used for analysis. All the known commercial instruments are currently using light scattering for particle sizing and fluorescence detection for chemical recognition. However, vibrational spectroscopy is the only non-invasive optical spectroscopy tool, which has proven to provide chemically-specific information about the interrogated sample. It is proposed that vibrational spectroscopy, based on nonlinear Raman scattering can be used to serve as an analytical tool for cytometry by providing rapid and accurate chemical recognition of flowing materials. To achieve a desired speed (>10,000 cell/particles per second), we have substantially upgraded our previous system for nonlinear Raman microspectroscopy. By increasing the size of the excitation volume to the size of a cell and by keeping the incident intensity at the same level, a dramatic increase of the nonlinear Raman signal is achieved. This allows high-quality vibrational spectra to be acquired within 10-100 microsecond from a single yeast cell without any observable damage to the irradiated cell. This is four orders of magnitude better than any previous attempts involving Raman microspectroscopy.

  1. Numerical methods for turbulent flow

    NASA Technical Reports Server (NTRS)

    Turner, James C., Jr.

    1988-01-01

    It has generally become accepted that the Navier-Strokes equations predict the dynamic behavior of turbulent as well as laminar flows of a fluid at a point in space away form a discontinuity such as a shock wave. Turbulence is also closely related to the phenomena of non-uniqueness of solutions of the Navier-Strokes equations. These second order, nonlinear partial differential equations can be solved analytically for only a few simple flows. Turbulent flow fields are much to complex to lend themselves to these few analytical methods. Numerical methods, therefore, offer the only possibility of achieving a solution of turbulent flow equations. In spite of recent advances in computer technology, the direct solution, by discrete methods, of the Navier-Strokes equations for turbulent flow fields is today, and in the foreseeable future, impossible. Thus the only economically feasible way to solve practical turbulent flow problems numerically is to use statistically averaged equations governing mean-flow quantities. The objective is to study some recent developments relating to the use of numerical methods to study turbulent flow.

  2. Flow analysis system and method

    NASA Technical Reports Server (NTRS)

    Hill, Wayne S. (Inventor); Barck, Bruce N. (Inventor)

    1998-01-01

    A non-invasive flow analysis system and method wherein a sensor, such as an acoustic sensor, is coupled to a conduit for transmitting a signal which varies depending on the characteristics of the flow in the conduit. The signal is amplified and there is a filter, responsive to the sensor signal, and tuned to pass a narrow band of frequencies proximate the resonant frequency of the sensor. A demodulator generates an amplitude envelope of the filtered signal and a number of flow indicator quantities are calculated based on variations in amplitude of the amplitude envelope. A neural network, or its equivalent, is then used to determine the flow rate of the flow in the conduit based on the flow indicator quantities.

  3. Vortex methods for separated flows

    NASA Technical Reports Server (NTRS)

    Spalart, Philippe R.

    1988-01-01

    The numerical solution of the Euler or Navier-Stokes equations by Lagrangian vortex methods is discussed. The mathematical background is presented and includes the relationship with traditional point-vortex studies, convergence to smooth solutions of the Euler equations, and the essential differences between two and three-dimensional cases. The difficulties in extending the method to viscous or compressible flows are explained. Two-dimensional flows around bluff bodies are emphasized. Robustness of the method and the assessment of accuracy, vortex-core profiles, time-marching schemes, numerical dissipation, and efficient programming are treated. Operation counts for unbounded and periodic flows are given, and two algorithms designed to speed up the calculations are described.

  4. Projection Methods for Interdendritic Flows

    SciTech Connect

    Han, Q.; Sabau, A.S.; Viswanathan, S.

    1999-02-28

    In spite of recent advances in the mathematical modeling of fluid dynamics for materials processing applications, no significant advances have been made in the numerical discretization of these equations. In this work, the application of two-step projection methods for the numerical simulation of interdendritic flows is, discussed. Unlike previous methods, the methods presented here are constructed for the exact equations which are characterized by variable density and volumetric fraction of the liquid. The drag terms, which describe the momentum loss due to the flow around and through the dendrite structures, are treated implicitly. Numerical examples for shrinkage-induced flow during solidification of an AI-4.5% Cu alloy bar is used to illustrate the effectiveness of the proposed algorithm.

  5. Vortex methods for separated flows

    NASA Technical Reports Server (NTRS)

    Spalart, Philippe R.

    1988-01-01

    The numerical solution of the Euler or Navier-Stokes equations by Lagrangian vortex methods is discussed. The mathematical background is presented in an elementary fashion and includes the relationship with traditional point-vortex studies, the convergence to smooth solutions of the Euler equations, and the essential differences between two- and three-dimensional cases. The difficulties in extending the method to viscous or compressible flows are explained. The overlap with the excellent review articles available is kept to a minimum and more emphasis is placed on the area of expertise, namely two-dimensional flows around bluff bodies. When solid walls are present, complete mathematical models are not available and a more heuristic attitude must be adopted. The imposition of inviscid and viscous boundary conditions without conformal mappings or image vortices and the creation of vorticity along solid walls are examined in detail. Methods for boundary-layer treatment and the question of the Kutta condition are discussed. Practical aspects and tips helpful in creating a method that really works are explained. The topics include the robustness of the method and the assessment of accuracy, vortex-core profiles, timemarching schemes, numerical dissipation, and efficient programming. Calculations of flows past streamlined or bluff bodies are used as examples when appropriate.

  6. Flow methods in chiral analysis.

    PubMed

    Trojanowicz, Marek; Kaniewska, Marzena

    2013-11-01

    The methods used for the separation and analytical determination of individual isomers are based on interactions with substances exhibiting optical activity. The currently used methods for the analysis of optically active compounds are primarily high-performance separation methods, such as gas and liquid chromatography using chiral stationary phases or chiral selectors in the mobile phase, and highly efficient electromigration techniques, such as capillary electrophoresis using chiral selectors. Chemical sensors and biosensors may also be designed for the analysis of optically active compounds. As enantiomers of the same compound are characterised by almost identical physico-chemical properties, their differentiation/separation in one-step unit operation in steady-state or dynamic flow systems requires the use of highly effective chiral selectors. Examples of such determinations are reviewed in this paper, based on 105 references. The greatest successes for isomer determination involve immunochemical interactions, enantioselectivity of the enzymatic biocatalytic processes, and interactions with ion-channel receptors or molecularly imprinted polymers. Conducting such processes under dynamic flow conditions may significantly enhance the differences in the kinetics of such processes, leading to greater differences in the signals recorded for enantiomers. Such determinations in flow conditions are effectively performed using surface-plasmon resonance and piezoelectric detections, as well as using common spectroscopic and electrochemical detections.

  7. Krylov methods for compressible flows

    NASA Technical Reports Server (NTRS)

    Tidriri, M. D.

    1995-01-01

    We investigate the application of Krylov methods to compressible flows, and the effect of implicit boundary conditions on the implicit solution of nonlinear problems. Two defect-correction procedures, namely, approximate factorization (AF) for structured grids and ILU/GMRES for general grids, are considered. Also considered here are Newton-Krylov matrix-free methods that we combined with the use of mixed discretization schemes in the implicitly defined Jacobian and its preconditioner. Numerical experiments that show the performance of our approaches are then presented.

  8. Flow Cytometry-Based Classification in Cancer Research: A View on Feature Selection

    PubMed Central

    Hassan, S. Sakira; Ruusuvuori, Pekka; Latonen, Leena; Huttunen, Heikki

    2015-01-01

    In this paper, we study the problem of feature selection in cancer-related machine learning tasks. In particular, we study the accuracy and stability of different feature selection approaches within simplistic machine learning pipelines. Earlier studies have shown that for certain cases, the accuracy of detection can easily reach 100% given enough training data. Here, however, we concentrate on simplifying the classification models with and seek for feature selection approaches that are reliable even with extremely small sample sizes. We show that as much as 50% of features can be discarded without compromising the prediction accuracy. Moreover, we study the model selection problem among the ℓ1 regularization path of logistic regression classifiers. To this aim, we compare a more traditional cross-validation approach with a recently proposed Bayesian error estimator. PMID:27081305

  9. Insertable fluid flow passage bridgepiece and method

    DOEpatents

    Jones, Daniel O.

    2000-01-01

    A fluid flow passage bridgepiece for insertion into an open-face fluid flow channel of a fluid flow plate is provided. The bridgepiece provides a sealed passage from a columnar fluid flow manifold to the flow channel, thereby preventing undesirable leakage into and out of the columnar fluid flow manifold. When deployed in the various fluid flow plates that are used in a Proton Exchange Membrane (PEM) fuel cell, bridgepieces of this invention prevent mixing of reactant gases, leakage of coolant or humidification water, and occlusion of the fluid flow channel by gasket material. The invention also provides a fluid flow plate assembly including an insertable bridgepiece, a fluid flow plate adapted for use with an insertable bridgepiece, and a method of manufacturing a fluid flow plate with an insertable fluid flow passage bridgepiece.

  10. Gas flow meter and method for measuring gas flow rate

    DOEpatents

    Robertson, Eric P.

    2006-08-01

    A gas flow rate meter includes an upstream line and two chambers having substantially equal, fixed volumes. An adjustable valve may direct the gas flow through the upstream line to either of the two chambers. A pressure monitoring device may be configured to prompt valve adjustments, directing the gas flow to an alternate chamber each time a pre-set pressure in the upstream line is reached. A method of measuring the gas flow rate measures the time required for the pressure in the upstream line to reach the pre-set pressure. The volume of the chamber and upstream line are known and fixed, thus the time required for the increase in pressure may be used to determine the flow rate of the gas. Another method of measuring the gas flow rate uses two pressure measurements of a fixed volume, taken at different times, to determine the flow rate of the gas.

  11. Use of flow cytometry for high-throughput cell population estimates in brain tissue

    PubMed Central

    Young, Nicole A.; Flaherty, David K.; Airey, David C.; Varlan, Peter; Aworunse, Feyi; Kaas, Jon H.; Collins, Christine E.

    2012-01-01

    The large size of primate brains is an impediment to obtaining high-resolution cell number maps of the cortex in humans and non-human primates. We present a rapid, flow cytometry-based cell counting method that can be used to estimate cell numbers from homogenized brain tissue samples comprising the entire cortical sheet. The new method, called the flow fractionator, is based on the isotropic fractionator (IF) method (Herculano-Houzel and Lent, 2005), but substitutes flow cytometry analysis for manual, microscope analysis using a Neubauer counting chamber. We show that our flow cytometry-based method for total cell estimation in homogenized brain tissue provides comparable data to that obtained using a counting chamber on a microscope. The advantages of the flow fractionator over existing methods are improved precision of cell number estimates and improved speed of analysis. PMID:22798947

  12. Use of flow cytometry for high-throughput cell population estimates in brain tissue.

    PubMed

    Young, Nicole A; Flaherty, David K; Airey, David C; Varlan, Peter; Aworunse, Feyi; Kaas, Jon H; Collins, Christine E

    2012-01-01

    The large size of primate brains is an impediment to obtaining high-resolution cell number maps of the cortex in humans and non-human primates. We present a rapid, flow cytometry-based cell counting method that can be used to estimate cell numbers from homogenized brain tissue samples comprising the entire cortical sheet. The new method, called the flow fractionator, is based on the isotropic fractionator (IF) method (Herculano-Houzel and Lent, 2005), but substitutes flow cytometry analysis for manual, microscope analysis using a Neubauer counting chamber. We show that our flow cytometry-based method for total cell estimation in homogenized brain tissue provides comparable data to that obtained using a counting chamber on a microscope. The advantages of the flow fractionator over existing methods are improved precision of cell number estimates and improved speed of analysis.

  13. A survey of aftbody flow prediction methods

    NASA Technical Reports Server (NTRS)

    Putnam, L. E.; Mace, J.

    1981-01-01

    A survey of computational methods used in the calculation of nozzle aftbody flows is presented. One class of methods reviewed are those which patch together solutions for the inviscid, boundary layer, and plume flow regions. The second class of methods reviewed are those which computationally solve the Navier Stokes equations over nozzle aftbodies with jet exhaust flow. Computed results from the methods are compared with experiment. Advantages and disadvantages of the various methods are discussed along with opportunities for further development of these methods.

  14. Cross flow electrofilter and method

    DOEpatents

    Gidaspow, Dimitri; Lee, Chang H.; Wasan, Darsh T.

    1981-01-01

    A filter for clarifying carbonaceous liquids containing finely divided solid particles of, for instance, unreacted coal, ash and other solids discharged from a coal liquefaction process is presented. The filter includes two passageways separated by a porous filter medium. In one preferred embodiment the filter medium is of tubular shape to form the first passageway and is enclosed within an outer housing to form the second passageway within the annulus. An electrode disposed in the first passageway, for instance along the tube axis, is connected to a source of high voltage for establishing an electric field between the electrode and the filter medium. Slurry feed flows through the first passageway tangentially to the surfaces of the filter medium and the electrode. Particles from the feed slurry are attracted to the electrode within the first passageway to prevent plugging of the porous filter medium while carbonaceous liquid filters into the second passageway for withdrawal. Concentrated slurry is discharged from the first passageway at an end opposite to the feed slurry inlet. Means are also provided for the addition of diluent and a surfactant into the slurry to control relative permittivity and the electrophoretic mobility of the particles.

  15. Level 2 validation of a flow cytometric method for detection of Escherichia coli O157:H7 in raw spinach.

    PubMed

    Williams, Anna J; Cooper, Willie M; Summage-West, Christine V; Sims, Lillie M; Woodruff, Robert; Christman, Jessica; Moskal, Ted J; Ramsaroop, Shawn; Sutherland, John B; Alusta, Pierre; Wilkes, Jon G; Buzatu, Dan A

    2015-12-23

    The Bacteriological Analytical Manual (BAM) method currently used by the United States Food and Drug Administration (FDA) to detect Escherichia coli O157:H7 in spinach was systematically compared to a new flow cytometry based method. This Food and Drug Administration (FDA) level 2 external laboratory validation study was designed to determine the latter method's sensitivity and speed for analysis of this pathogen in raw spinach. Detection of target cell inoculations with a low cell count is critical, since enterohemorrhagic strains of E. coli require an infective dose of as few as 10 cells (Schmid-Hempel and Frank, 2007). Although, according to the FDA, the infectious dose is unknown (Food and Drug Administration, 1993). Therefore, the inoculation level into the spinach, a total of 2.0±2.6 viable E. coli O157 cells, was specified to yield between 25% and 75% detection by the new method, out of 20 samples (10 positives and 10 negatives). This criterion was met in that the new method detected 60% of the nominally positive samples; the corresponding sensitivity of the reference method was 50%. For both methods the most likely explanation for false negatives was that no viable cells were actually introduced into the sample. In this validation study, the flow cytometry method was equal to the BAM in sensitivity and far superior in speed. Published by Elsevier B.V.

  16. The Direct Method of Cash Flows.

    ERIC Educational Resources Information Center

    Bosserman, David C.; Fischer, Mary

    2000-01-01

    Explains to college/university business officers how to comply with Governmental Accounting Standards Board Statements Nos. 34, 35, and 9, which require the direct method of presenting cash flows from operating activities and reconciliation of operating cash flows to operating income by fiscal year 2001. Institutions are urged to begin immediately…

  17. The Direct Method of Cash Flows.

    ERIC Educational Resources Information Center

    Bosserman, David C.; Fischer, Mary

    2000-01-01

    Explains to college/university business officers how to comply with Governmental Accounting Standards Board Statements Nos. 34, 35, and 9, which require the direct method of presenting cash flows from operating activities and reconciliation of operating cash flows to operating income by fiscal year 2001. Institutions are urged to begin immediately…

  18. Computational methods for unsteady transonic flows

    NASA Technical Reports Server (NTRS)

    Edwards, John W.; Thomas, J. L.

    1987-01-01

    Computational methods for unsteady transonic flows are surveyed with emphasis on prediction. Computational difficulty is discussed with respect to type of unsteady flow; attached, mixed (attached/separated) and separated. Significant early computations of shock motions, aileron buzz and periodic oscillations are discussed. The maturation of computational methods towards the capability of treating complete vehicles with reasonable computational resources is noted and a survey of recent comparisons with experimental results is compiled. The importance of mixed attached and separated flow modeling for aeroelastic analysis is discussed, and recent calculations of periodic aerodynamic oscillations for an 18 percent thick circular arc airfoil are given.

  19. Tomographic methods in flow diagnostics

    NASA Technical Reports Server (NTRS)

    Decker, Arthur J.

    1993-01-01

    This report presents a viewpoint of tomography that should be well adapted to currently available optical measurement technology as well as the needs of computational and experimental fluid dynamists. The goals in mind are to record data with the fastest optical array sensors; process the data with the fastest parallel processing technology available for small computers; and generate results for both experimental and theoretical data. An in-depth example treats interferometric data as it might be recorded in an aeronautics test facility, but the results are applicable whenever fluid properties are to be measured or applied from projections of those properties. The paper discusses both computed and neural net calibration tomography. The report also contains an overview of key definitions and computational methods, key references, computational problems such as ill-posedness, artifacts, missing data, and some possible and current research topics.

  20. Asymptotic methods for internal transonic flows

    NASA Technical Reports Server (NTRS)

    Adamson, T. C., Jr.; Messiter, A. F.

    1989-01-01

    For many internal transonic flows of practical interest, some of the relevant nondimensional parameters typically are small enough that a perturbation scheme can be expected to give a useful level of numerical accuracy. A variety of steady and unsteady transonic channel and cascade flows is studied with the help of systematic perturbation methods which take advantage of this fact. Asymptotic representations are constructed for small changes in channel cross-section area, small flow deflection angles, small differences between the flow velocity and the sound speed, small amplitudes of imposed oscillations, and small reduced frequencies. Inside a channel the flow is nearly one-dimensional except in thin regions immediately downstream of a shock wave, at the channel entrance and exit, and near the channel throat. A study of two-dimensional cascade flow is extended to include a description of three-dimensional compressor-rotor flow which leads to analytical results except in thin edge regions which require numerical solution. For unsteady flow the qualitative nature of the shock-wave motion in a channel depends strongly on the orders of magnitude of the frequency and amplitude of impressed wall oscillations or fluctuations in back pressure. One example of supersonic flow is considered, for a channel with length large compared to its width, including the effect of separation bubbles and the possibility of self-sustained oscillations. The effect of viscosity on a weak shock wave in a channel is discussed.

  1. Finite element methods for high speed flows

    NASA Technical Reports Server (NTRS)

    Loehner, R.; Morgan, K.; Peraire, J.; Zienkiewicz, O. C.

    1985-01-01

    An explicit finite element based solution procedure for solving the equations of compressible viscous high speed flow is presented. The method uses domain splitting to advance the solution with different timesteps on different portions of the mesh. For steady inviscid flows, adaptive mesh refinement procedures are successfully employed to enhance the definition of discontinuities. Preliminary ideas on the application of adaptive mesh refinement to the solution of problems involving steady viscous flow are presented. Sample timings are given for the performance of the finite element code on modern supercomputers.

  2. An exact inverse method for subsonic flows

    NASA Technical Reports Server (NTRS)

    Daripa, Prabir

    1988-01-01

    A new inverse method for the aerodynamic design of airfoils is presented for subcritical flows. The pressure distribution in this method can be prescribed as a function of the arclength of the still unknown body. It is shown that this inverse problem is mathematically equivalent to solving only one nonlinear boundary value problem subject to known Dirichlet data on the boundary.

  3. Background field method in the gradient flow

    NASA Astrophysics Data System (ADS)

    Suzuki, Hiroshi

    2015-10-01

    In perturbative consideration of the Yang-Mills gradient flow, it is useful to introduce a gauge non-covariant term (“gauge-fixing term”) to the flow equation that gives rise to a Gaussian damping factor also for gauge degrees of freedom. In the present paper, we consider a modified form of the gauge-fixing term that manifestly preserves covariance under the background gauge transformation. It is shown that our gauge-fixing term does not affect gauge-invariant quantities as does the conventional gauge-fixing term. The formulation thus allows a background gauge covariant perturbative expansion of the flow equation that provides, in particular, a very efficient computational method of expansion coefficients in the small flow time expansion. The formulation can be generalized to systems containing fermions.

  4. Method and apparatus for controlling fluid flow

    DOEpatents

    Miller, J.R.

    1980-06-27

    A method and apparatus for precisely controlling the rate (and hence amount) of fluid flow are given. The controlled flow rate is finely adjustable, can be extremely small (on the order of microliter-atmospheres per second), can be adjusted to zero (flow stopped), and is stable to better than 1% with time. The dead volume of the valve can be made arbitrarily small, in fact essentially zero. The valve employs no wearing mechanical parts (including springs, stems, or seals). The valve is finely adjustable, has a flow rate dynamic range of many decades, can be made compatible with any fluid, and is suitable for incorporation into an open or closed loop servo-control system.

  5. Lattice Boltzmann methods for moving boundary flows

    NASA Astrophysics Data System (ADS)

    Inamuro, Takaji

    2012-04-01

    The lattice Boltzmann methods (LBMs) for moving boundary flows are presented. The LBM for two-phase fluid flows with the same density and the LBM combined with the immersed boundary method are described. In addition, the LBM on a moving multi-block grid is explained. Three numerical examples (a droplet moving in a constricted tube, the lift generation of a flapping wing and the sedimentation of an elliptical cylinder) are shown in order to demonstrate the applicability of the LBMs to moving boundary problems.

  6. Cytometry-based single-cell analysis of intact epithelial signaling reveals MAPK activation divergent from TNF-α-induced apoptosis in vivo

    PubMed Central

    Simmons, Alan J; Banerjee, Amrita; McKinley, Eliot T; Scurrah, Cherie' R; Herring, Charles A; Gewin, Leslie S; Masuzaki, Ryota; Karp, Seth J; Franklin, Jeffrey L; Gerdes, Michael J; Irish, Jonathan M; Coffey, Robert J; Lau, Ken S

    2015-01-01

    Understanding heterogeneous cellular behaviors in a complex tissue requires the evaluation of signaling networks at single-cell resolution. However, probing signaling in epithelial tissues using cytometry-based single-cell analysis has been confounded by the necessity of single-cell dissociation, where disrupting cell-to-cell connections inherently perturbs native cell signaling states. Here, we demonstrate a novel strategy (Disaggregation for Intracellular Signaling in Single Epithelial Cells from Tissue—DISSECT) that preserves native signaling for Cytometry Time-of-Flight (CyTOF) and fluorescent flow cytometry applications. A 21-plex CyTOF analysis encompassing core signaling and cell-identity markers was performed on the small intestinal epithelium after systemic tumor necrosis factor-alpha (TNF-α) stimulation. Unsupervised and supervised analyses robustly selected signaling features that identify a unique subset of epithelial cells that are sensitized to TNF-α-induced apoptosis in the seemingly homogeneous enterocyte population. Specifically, p-ERK and apoptosis are divergently regulated in neighboring enterocytes within the epithelium, suggesting a mechanism of contact-dependent survival. Our novel single-cell approach can broadly be applied, using both CyTOF and multi-parameter flow cytometry, for investigating normal and diseased cell states in a wide range of epithelial tissues. PMID:26519361

  7. Method for identifying anomalous terrestrial heat flows

    DOEpatents

    Del Grande, Nancy Kerr

    1977-01-25

    A method for locating and mapping the magnitude and extent of terrestrial heat-flow anomalies from 5 to 50 times average with a tenfold improved sensitivity over orthodox applications of aerial temperature-sensing surveys as used for geothermal reconnaissance. The method remotely senses surface temperature anomalies such as occur from geothermal resources or oxidizing ore bodies by: measuring the spectral, spatial, statistical, thermal, and temporal features characterizing infrared radiation emitted by natural terrestrial surfaces; deriving from these measurements the true surface temperature with uncertainties as small as 0.05 to 0.5 K; removing effects related to natural temperature variations of topographic, hydrologic, or meteoric origin, the surface composition, detector noise, and atmospheric conditions; factoring out the ambient normal-surface temperature for non-thermally enhanced areas surveyed under otherwise identical environmental conditions; distinguishing significant residual temperature enhancements characteristic of anomalous heat flows and mapping the extent and magnitude of anomalous heat flows where they occur.

  8. Advanced overset methods for vortex dominated flows

    NASA Astrophysics Data System (ADS)

    Foster, Norman F.

    A newly implemented computational method of high-order accuracy is presented for the accurate calculation of unsteady vortical structures that may produce aeroacoustic sources, or affect downstream structural responses. The method involves prediction of the mean flow field by solving the Navier-Stokes equations (NSE) using a computational fluid dynamics (CFD) solver that employs high-order discretization on overlapping (overset) grid systems. The method dramatically reduces the artificial dissipation and dispersion of vortical flow features that would ordinarily be lost or degraded with the use of current methods. Complex domains are discretized using an overset grid strategy that allows for the use of multiple high quality structured meshes. The high-order method is developed and incorporated into a generalized overset grid assembly scheme, which allows high-order spatial accuracy of the NSE solutions to be maintained across overset grid boundaries. Comparisons are made to calculations that do not preserve high-order accuracy at overset boundaries, and insight is obtained into the effects and sensitivities of different treatments of overlapping boundaries. A nested block adaptive mesh refinement (AMR) method has also been developed, within the context of the overset paradigm. The method is shown to significantly improve accuracy for a given computational cell count by tracking dynamic vortical features using appropriate dynamic refinement and coarsening, and its implementation in the context of the high-order overset method is presented. The computational procedures presented herein are tested against analytic and canonical cases (slightly compressible, M ≤ 0.5, and incompressible mean flows) in order to characterize the accuracy of flow field calculations using high-order discretization and overset schemes across overlapping grid boundaries. The methods are also extended to far more complex systems including the transport of rotorcraft hub vorticity to

  9. Development of a novel cell sorting method that samples population diversity in flow cytometry.

    PubMed

    Osborne, Geoffrey W; Andersen, Stacey B; Battye, Francis L

    2015-11-01

    Flow cytometry based electrostatic cell sorting is an important tool in the separation of cell populations. Existing instruments can sort single cells into multi-well collection plates, and keep track of cell of origin and sorted well location. However currently single sorted cell results reflect the population distribution and fail to capture the population diversity. Software was designed that implements a novel sorting approach, "Slice and Dice Sorting," that links a graphical representation of a multi-well plate to logic that ensures that single cells are sampled and sorted from all areas defined by the sort region/s. Therefore the diversity of the total population is captured, and the more frequently occurring or rarer cell types are all sampled. The sorting approach was tested computationally, and using functional cell based assays. Computationally we demonstrate that conventional single cell sorting can sample as little as 50% of the population diversity dependant on the population distribution, and that Slice and Dice sorting samples much more of the variety present within a cell population. We then show by sorting single cells into wells using the Slice and Dice sorting method that there are cells sorted using this method that would be either rarely sorted, or not sorted at all using conventional single cell sorting approaches. The present study demonstrates a novel single cell sorting method that samples much more of the population diversity than current methods. It has implications in clonal selection, stem cell sorting, single cell sequencing and any areas where population heterogeneity is of importance.

  10. Computational Methods for Complex Flow Fields.

    DTIC Science & Technology

    1986-06-28

    James J. Riley Joel H . Ferziger "Turbulent Flow Simulation - Future Needs" Micha Wolfshtein " Numerical Calculation of the Reynolds Stress and Turbulent...July 1983. Also in RECENT ADVANCES IN NUMERICAL METHODS IN FLUIDS, Vol. 3, Editor W.G. Habashi, Pineridge Press. 2. Usab, W.J., "Embedded Mesh Solutions...ridiaconal matrices applicable to approximane factorization methods . E:xlicit algcrit-s are also easier to adapz to multiProcessor arcr.itectures as the

  11. Variational adaptive correlation method for flow estimation.

    PubMed

    Becker, Florian; Wieneke, Bernhard; Petra, Stefania; Schröder, Andreas; Schnörr, Christoph

    2012-06-01

    A variational approach is presented to the estimation of turbulent fluid flow from particle image sequences in experimental fluid mechanics. The approach comprises two coupled optimizations for adapting size and shape of a Gaussian correlation window at each location and for estimating the flow, respectively. The method copes with a wide range of particle densities and image noise levels without any data-specific parameter tuning. Based on a careful implementation of a multiscale nonlinear optimization technique, we demonstrate robustness of the solution over typical experimental scenarios and highest estimation accuracy for an international benchmark data set (PIV Challenge).

  12. Hydraulic flow visualization method and apparatus

    DOEpatents

    Karidis, P.G.

    1984-01-01

    An apparatus and method for visualizing liquid flow. Pulses of gas bubbles are introduced into a liquid flow stream and a strobe light is operated at a frequency related to the frequency of the gas pulses to shine on the bubbles as they pass through the liquid stream. The gas pulses pass through a probe body having a valve element, and a reciprocating valve stem passes through the probe body to operate the valve element. A stem actuating device comprises a slidable reciprocating member, operated by a crank arm. The actuated member is adjustable to adjust the amount of the valve opening during each pulse.

  13. Hydraulic flow visualization method and apparatus

    SciTech Connect

    Karidis, P.G.

    1984-12-11

    An apparatus and method for visualizing liquid flow. Pulses of gas bubbles are introduced into a liquid flow stream and a strobe light is operated at a frequency related to the frequency of the gas pulses to shine on the bubbles as they pass through the liquid stream. The gas pulses pass through a probe body having a valve element, and a reciprocating valve stem passes through the probe body to operate the valve element. A stem actuating device comprises a slidable reciprocating member, operated by a crank arm. The actuated member is adjustable to adjust the amount of the valve opening during each pulse.

  14. Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.

    PubMed

    Fine, Noah; Barzilay, Oriyah; Glogauer, Michael

    2017-01-01

    Neutrophils are primary phagocytes that recognize their targets through surface chemistry, either through Pattern Recognition Receptor (PPR) interaction with Pathogen-Associated Molecular Patterns (PAMPs) or through immunoglobulin (Ig) or complement mediated recognition. Opsonization can be important for target recognition, and phagocytosis by neutrophils in whole blood can be greatly enhanced due to the presence of blood serum components and platelets. Powerful and sensitive flow cytometry based methods are presented to measure phagocytosis by human blood neutrophils and mouse peritoneal neutrophils.

  15. Flow coating apparatus and method of coating

    DOEpatents

    Hanumanthu, Ramasubrahmaniam; Neyman, Patrick; MacDonald, Niles; Brophy, Brenor; Kopczynski, Kevin; Nair, Wood

    2014-03-11

    Disclosed is a flow coating apparatus, comprising a slot that can dispense a coating material in an approximately uniform manner along a distribution blade that increases uniformity by means of surface tension and transfers the uniform flow of coating material onto an inclined substrate such as for example glass, solar panels, windows or part of an electronic display. Also disclosed is a method of flow coating a substrate using the apparatus such that the substrate is positioned correctly relative to the distribution blade, a pre-wetting step is completed where both the blade and substrate are completed wetted with a pre-wet solution prior to dispensing of the coating material onto the distribution blade from the slot and hence onto the substrate. Thereafter the substrate is removed from the distribution blade and allowed to dry, thereby forming a coating.

  16. Method of fabricating a flow device

    DOEpatents

    Hale, Robert L.

    1978-01-01

    This invention is a novel method for fabricating leak-tight tubular articles which have an interior flow channel whose contour must conform very closely with design specifications but which are composed of metal which tends to warp if welded. The method comprises designing two longitudinal half-sections of the article, the half-sections being contoured internally to cooperatively form the desired flow passageway. Each half-section is designed with a pair of opposed side flanges extending between the end flanges and integral therewith. The half-sections are positioned with their various flanges in confronting relation and with elongated metal gaskets extending between the confronting flanges for the length of the array. The gaskets are a deformable metal which is fusion-weldable to the end flanges. The mating side flanges are joined mechanically to deform the gaskets and provide a longitudinally sealed assembly. The portions of the end flanges contiguous with the ends of the gaskets then are welded to provide localized end welds which incorporate ends of the gaskets, thus transversely sealing the assembly. This method of fabrication provides leak-tight articles having the desired precisely contoured flow channels, whereas various conventional methods have been found unsatisfactory.

  17. Gauge finite element method for incompressible flows

    NASA Astrophysics Data System (ADS)

    E, Weinan; Liu, Jian-Guo

    2000-12-01

    A finite element method for computing viscous incompressible flows based on the gauge formulation introduced in [Weinan E, Liu J-G. Gauge method for viscous incompressible flows. Journal of Computational Physics (submitted)] is presented. This formulation replaces the pressure by a gauge variable. This new gauge variable is a numerical tool and differs from the standard gauge variable that arises from decomposing a compressible velocity field. It has the advantage that an additional boundary condition can be assigned to the gauge variable, thus eliminating the issue of a pressure boundary condition associated with the original primitive variable formulation. The computational task is then reduced to solving standard heat and Poisson equations, which are approximated by straightforward, piecewise linear (or higher-order) finite elements. This method can achieve high-order accuracy at a cost comparable with that of solving standard heat and Poisson equations. It is naturally adapted to complex geometry and it is much simpler than traditional finite element methods for incompressible flows. Several numerical examples on both structured and unstructured grids are presented. Copyright

  18. A topological method for vortex identification in turbulent flows

    NASA Astrophysics Data System (ADS)

    Zhong, Qiang; Chen, Qigang; Chen, Huai; Li, Danxun

    2017-02-01

    We present a novel vortex identification method based on structured vorticity (ω s) of the direction field of flow (velocity vectors set to unit magnitude). As a direct measure of streamline curvature is insensitive to vortex strength, ω s is effective in detecting vortices of various strengths. The effectiveness has been tested against both analytical flows (pure shear flow, Oseen vortex flow, strong outward spiraling motion, straining flow, Taylor-Green flow) and experimental flows (closed cavity flow, closed and open channel flow). Comparison of the new method with the swirling-strength method indicates that the new method shows promise as being a simple and effective criterion for vortex identification.

  19. Numerical Methods For Chemically Reacting Flows

    NASA Technical Reports Server (NTRS)

    Leveque, R. J.; Yee, H. C.

    1990-01-01

    Issues related to numerical stability, accuracy, and resolution discussed. Technical memorandum presents issues in numerical solution of hyperbolic conservation laws containing "stiff" (relatively large and rapidly changing) source terms. Such equations often used to represent chemically reacting flows. Usually solved by finite-difference numerical methods. Source terms generally necessitate use of small time and/or space steps to obtain sufficient resolution, especially at discontinuities, where incorrect mathematical modeling results in unphysical solutions.

  20. Lattice Boltzmann method and channel flow

    NASA Astrophysics Data System (ADS)

    Stensholt, Sigvat; Mongstad Hope, Sigmund

    2016-07-01

    Lattice Boltzmann methods are presented at an introductory level with a focus on fairly simple simulations that can be used to test and illustrate the model’s capabilities. Two scenarios are presented. The first is a simple laminar flow in a straight channel driven by a pressure gradient (Poiseuille flow). The second is a more complex, including a wedge where Moffatt vortices may be induced if the wedge is deep enough. Simulations of the Poiseuille flow scenario accurately capture the theoretical velocity profile. The experiment shows the location of the fluid-wall boundary and the effects viscosity has on the velocity and convergence time. The numerical capabilities of the lattice Boltzmann model are tested further by simulating the more complex Moffatt vortex scenario. The method reproduces with high accuracy the theoretical predction that Moffat vortices will not form in a wedge if the vertex angle exceeds 146°. Practical issues limitations of the lattice Boltzmann method are discussed. In particular the accuracy of the bounce-back boundary condition is first order dependent on the grid resolution.

  1. Computational methods for vortex dominated compressible flows

    NASA Technical Reports Server (NTRS)

    Murman, Earll M.

    1987-01-01

    The principal objectives were to: understand the mechanisms by which Euler equation computations model leading edge vortex flows; understand the vortical and shock wave structures that may exist for different wing shapes, angles of incidence, and Mach numbers; and compare calculations with experiments in order to ascertain the limitations and advantages of Euler equation models. The initial approach utilized the cell centered finite volume Jameson scheme. The final calculation utilized a cell vertex finite volume method on an unstructured grid. Both methods used Runge-Kutta four stage schemes for integrating the equations. The principal findings are briefly summarized.

  2. A continuous method for gene flow.

    PubMed

    Palczewski, Michal; Beerli, Peter

    2013-07-01

    Most modern population genetics inference methods are based on the coalescence framework. Methods that allow estimating parameters of structured populations commonly insert migration events into the genealogies. For these methods the calculation of the coalescence probability density of a genealogy requires a product over all time periods between events. Data sets that contain populations with high rates of gene flow among them require an enormous number of calculations. A new method, transition probability-structured coalescence (TPSC), replaces the discrete migration events with probability statements. Because the speed of calculation is independent of the amount of gene flow, this method allows calculating the coalescence densities efficiently. The current implementation of TPSC uses an approximation simplifying the interaction among lineages. Simulations and coverage comparisons of TPSC vs. MIGRATE show that TPSC allows estimation of high migration rates more precisely, but because of the approximation the estimation of low migration rates is biased. The implementation of TPSC into programs that calculate quantities on phylogenetic tree structures is straightforward, so the TPSC approach will facilitate more general inferences in many computer programs.

  3. Flow cytometric detection method for DNA samples

    DOEpatents

    Nasarabadi, Shanavaz [Livermore, CA; Langlois, Richard G [Livermore, CA; Venkateswaran, Kodumudi S [Round Rock, TX

    2011-07-05

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.

  4. Flow cytometric detection method for DNA samples

    DOEpatents

    Nasarabadi, Shanavaz; Langlois, Richard G.; Venkateswaran, Kodumudi S.

    2006-08-01

    Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.

  5. An approximate projection method for incompressible flow

    NASA Astrophysics Data System (ADS)

    Stevens, David E.; Chan, Stevens T.; Gresho, Phil

    2002-12-01

    This paper presents an approximate projection method for incompressible flows. This method is derived from Galerkin orthogonality conditions using equal-order piecewise linear elements for both velocity and pressure, hereafter Q1Q1. By combining an approximate projection for the velocities with a variational discretization of the continuum pressure Poisson equation, one eliminates the need to filter either the velocity or pressure fields as is often needed with equal-order element formulations. This variational approach extends to multiple types of elements; examples and results for triangular and quadrilateral elements are provided. This method is related to the method of Almgren et al. (SIAM J. Sci. Comput. 2000; 22: 1139-1159) and the PISO method of Issa (J. Comput. Phys. 1985; 62: 40-65). These methods use a combination of two elliptic solves, one to reduce the divergence of the velocities and another to approximate the pressure Poisson equation. Both Q1Q1 and the method of Almgren et al. solve the second Poisson equation with a weak error tolerance to achieve more computational efficiency.A Fourier analysis of Q1Q1 shows that a consistent mass matrix has a positive effect on both accuracy and mass conservation. A numerical comparison with the widely used Q1Q0 (piecewise linear velocities, piecewise constant pressures) on a periodic test case with an analytic solution verifies this analysis. Q1Q1 is shown to have comparable accuracy as Q1Q0 and good agreement with experiment for flow over an isolated cubic obstacle and dispersion of a point source in its wake.

  6. Immersed boundary methods for viscoelastic particulate flows

    NASA Astrophysics Data System (ADS)

    Krishnan, Sreenath; Shaqfeh, Eric; Iaccarino, Gianluca

    2015-11-01

    Viscoelastic particulate suspensions play key roles in many energy applications. Our goal is to develop a simulation-based tool for engineering such suspensions. This study is concerned with fully resolved simulations, wherein all flow scales associated with the particle motion are resolved. The present effort is based on Immersed Boundary methods, in which the domain grids do not conform to particle geometry. In this approach, the conservation of momentum equations, which include both Newtonian and non-Newtonian stresses, are solved over the entire domain including the region occupied by the particles. The particles are defined on a separate Lagrangian mesh that is free to move over an underlying Eulerian grid. The development of an immersed boundary forcing technique for moving bodies within an unstructured-mesh, massively parallel, non-Newtonian flow solver is thus developed and described. The presentation will focus on the numerical algorithm and measures taken to enable efficient parallelization and transfer of information between the underlying fluid grid and the particle mesh. Several validation test cases will be presented including sedimentation under orthogonal shear - a key flow in drilling muds and fracking fluids.

  7. A Filtering Method For Gravitationally Stratified Flows

    SciTech Connect

    Gatti-Bono, Caroline; Colella, Phillip

    2005-04-25

    Gravity waves arise in gravitationally stratified compressible flows at low Mach and Froude numbers. These waves can have a negligible influence on the overall dynamics of the fluid but, for numerical methods where the acoustic waves are treated implicitly, they impose a significant restriction on the time step. A way to alleviate this restriction is to filter out the modes corresponding to the fastest gravity waves so that a larger time step can be used. This paper presents a filtering strategy of the fully compressible equations based on normal mode analysis that is used throughout the simulation to compute the fast dynamics and that is able to damp only fast gravity modes.

  8. Singularity embedding method in potential flow calculations

    NASA Technical Reports Server (NTRS)

    Jou, W. H.; Huynh, H.

    1982-01-01

    The so-called H-type mesh is used in a finite-element (or finite-volume) calculation of the potential flow past an airfoil. Due to coordinate singularity at the leading edge, a special singular trial function is used for the elements neighboring the leading edge. The results using the special singular elements are compared to those using the regular elements. It is found that the unreasonable pressure distribution obtained by the latter is removed by the embedding of the singular element. Suggestions to extend the present method to transonic cases are given.

  9. A power function method for estimating base flow.

    PubMed

    Lott, Darline A; Stewart, Mark T

    2013-01-01

    Analytical base flow separation techniques are often used to determine the base flow contribution to total stream flow. Most analytical methods derive base flow from discharge records alone without using basin-specific variables other than basin area. This paper derives a power function for estimating base flow, the form being aQ(b) + cQ, an analytical method calibrated against an integrated basin variable, specific conductance, relating base flow to total discharge, and is consistent with observed mathematical behavior of dissolved solids in stream flow with varying discharge. Advantages of the method are being uncomplicated, reproducible, and applicable to hydrograph separation in basins with limited specific conductance data. The power function relationship between base flow and discharge holds over a wide range of basin areas. It better replicates base flow determined by mass balance methods than analytical methods such as filters or smoothing routines that are not calibrated to natural tracers or empirical basin and gauge-specific variables. Also, it can be used with discharge during periods without specific conductance values, including separating base flow from quick flow for single events. However, it may overestimate base flow during very high flow events. Application of geochemical mass balance and power function base flow separation methods to stream flow and specific conductance records from multiple gauges in the same basin suggests that analytical base flow separation methods must be calibrated at each gauge. Using average values of coefficients introduces a potentially significant and unknown error in base flow as compared with mass balance methods.

  10. Transonic Flow Computations Using Nonlinear Potential Methods

    NASA Technical Reports Server (NTRS)

    Holst, Terry L.; Kwak, Dochan (Technical Monitor)

    2000-01-01

    This presentation describes the state of transonic flow simulation using nonlinear potential methods for external aerodynamic applications. The presentation begins with a review of the various potential equation forms (with emphasis on the full potential equation) and includes a discussion of pertinent mathematical characteristics and all derivation assumptions. Impact of the derivation assumptions on simulation accuracy, especially with respect to shock wave capture, is discussed. Key characteristics of all numerical algorithm types used for solving nonlinear potential equations, including steady, unsteady, space marching, and design methods, are described. Both spatial discretization and iteration scheme characteristics are examined. Numerical results for various aerodynamic applications are included throughout the presentation to highlight key discussion points. The presentation ends with concluding remarks and recommendations for future work. Overall. nonlinear potential solvers are efficient, highly developed and routinely used in the aerodynamic design environment for cruise conditions. Published by Elsevier Science Ltd. All rights reserved.

  11. Finite area method for nonlinear conical flows

    NASA Technical Reports Server (NTRS)

    Sritharan, S. S.; Seebass, A. R.

    1982-01-01

    A fully conservative finite area method for the computation of steady inviscid flow about general conical bodies at incidence is described. The procedure utilizes the potential approximation and implements a body conforming mesh generator. The conical potential is assumed to have its best linear variation inside each mesh cell and a secondary interlocking cell system is used to establish the flux balance required to conserve mass. In the supersonic regions the scheme is desymmetrized by adding appropriate artificial viscosity in conservation form. The algorithm is nearly an order of a magnitude faster than present Euler methods and predicts known results accurately and qualitative features such as nodal point lift off correctly. Results are compared with those of other investigations.

  12. Finite area method for nonlinear conical flows

    NASA Technical Reports Server (NTRS)

    Sritharan, S. S.; Seebass, A. R.

    1982-01-01

    A fully conservative finite area method for the computation of steady inviscid flow about general conical bodies at incidence is described. The procedure utilizes the potential approximation and implements a body conforming mesh generator. The conical potential is assumed to have its best linear variation inside each mesh cell and a secondary interlocking cell system is used to establish the flux balance required to conserve mass. In the supersonic regions the scheme is desymmetrized by adding appropriate artificial viscosity in conservation form. The algorithm is nearly an order of a magnitude faster than present Euler methods and predicts known results accurately and qualitative features such as nodal point lift off correctly. Results are compared with those of other investigations.

  13. Solution of plane cascade flow using improved surface singularity methods

    NASA Technical Reports Server (NTRS)

    Mcfarland, E. R.

    1981-01-01

    A solution method has been developed for calculating compressible inviscid flow through a linear cascade of arbitrary blade shapes. The method uses advanced surface singularity formulations which were adapted from those found in current external flow analyses. The resulting solution technique provides a fast flexible calculation for flows through turbomachinery blade rows. The solution method and some examples of the method's capabilities are presented.

  14. Implicit methods for computing chemically reacting flow

    NASA Astrophysics Data System (ADS)

    Li, C. P.

    Modeling the inviscid air flow and its constituents over a hypersonically flying body requires a large system of Euler and chemical rate equations in three spatial coordinates. In most cases, the simplest approach to solve for the variables would be based on explicit integration of the governing equations. But the standard techniques are not suitable for this purpose because the integration step size must be inordinately small in order to maintain numerical stability. The difficulty is due to the stiff character of the difference equations, as there exists a large spectrum of spatial and temporal scales in the approximation of physical phenomena by numerical methods. For instance, in the calculation of gradients caused by shock and by cooled wall on a coarse grid, unchecked numerical errors eventually will lead to violent instability, and in calculations of species near chemical equilibrium, a small error in one species will give rise to a large error in the source term for other species. Despite the different nature of the stiffness in a complex system of equations, the most effective approach is believed to be implicit integration. The step increment is no longer dictated by the stability criteria for explicit methods, but instead is dictated by the degree of linearization introduced to the governing equations and by the order of desired accuracy. The linearization is enacted by means of Jacobian matrices, resulting from the differentiation of the flux as well as the rate production terms with respect to dependent variables. The backward Euler scheme is then applied to discretize the partial differential equations and to convert them into a system of linear difference equations in vector form. As this particular approach has the A-stable property, it is the one recommended by Lomax and Bailey(1) for one-dimensional nonequilibrium flow studies. However, in the practice of solving flow problems in multidimensions, it was not clear then how to deal with the mammoth

  15. Flow “Fine” Synthesis: High Yielding and Selective Organic Synthesis by Flow Methods

    PubMed Central

    2015-01-01

    Abstract The concept of flow “fine” synthesis, that is, high yielding and selective organic synthesis by flow methods, is described. Some examples of flow “fine” synthesis of natural products and APIs are discussed. Flow methods have several advantages over batch methods in terms of environmental compatibility, efficiency, and safety. However, synthesis by flow methods is more difficult than synthesis by batch methods. Indeed, it has been considered that synthesis by flow methods can be applicable for the production of simple gasses but that it is difficult to apply to the synthesis of complex molecules such as natural products and APIs. Therefore, organic synthesis of such complex molecules has been conducted by batch methods. On the other hand, syntheses and reactions that attain high yields and high selectivities by flow methods are increasingly reported. Flow methods are leading candidates for the next generation of manufacturing methods that can mitigate environmental concerns toward sustainable society. PMID:26337828

  16. Flow "Fine" Synthesis: High Yielding and Selective Organic Synthesis by Flow Methods.

    PubMed

    Kobayashi, Shū

    2016-02-18

    The concept of flow "fine" synthesis, that is, high yielding and selective organic synthesis by flow methods, is described. Some examples of flow "fine" synthesis of natural products and APIs are discussed. Flow methods have several advantages over batch methods in terms of environmental compatibility, efficiency, and safety. However, synthesis by flow methods is more difficult than synthesis by batch methods. Indeed, it has been considered that synthesis by flow methods can be applicable for the production of simple gasses but that it is difficult to apply to the synthesis of complex molecules such as natural products and APIs. Therefore, organic synthesis of such complex molecules has been conducted by batch methods. On the other hand, syntheses and reactions that attain high yields and high selectivities by flow methods are increasingly reported. Flow methods are leading candidates for the next generation of manufacturing methods that can mitigate environmental concerns toward sustainable society.

  17. Sensitive cytometry based system for enumeration, capture and analysis of gene mutations of circulating tumor cells.

    PubMed

    Sawada, Takeshi; Watanabe, Masaru; Fujimura, Yuu; Yagishita, Shigehiro; Shimoyama, Tatsu; Maeda, Yoshiharu; Kanda, Shintaro; Yunokawa, Mayu; Tamura, Kenji; Tamura, Tomohide; Minami, Hironobu; Koh, Yasuhiro; Koizumi, Fumiaki

    2016-03-01

    Methods for the enumeration and molecular characterization of circulating tumor cells (CTC) have been actively investigated. However, such methods are still technically challenging. We have developed a novel epithelial cell adhesion molecule independent CTC enumeration system integrated with a sorting system using a microfluidics chip. We compared the number of CTC detected using our system with those detected using the CellSearch system in 46 patients with various cancers. We also evaluated epidermal growth factor receptor (EGFR) and PIK3CA mutations of captured CTC in a study of 4 lung cancer and 4 breast cancer patients. The percentage of samples with detected CTC was significantly higher with our system (65.2%) than with CellSearch (28.3%). The number of detected CTC per patient using our system was statistically higher than that using CellSearch (median 5, 0; P = 0.000172, Wilcoxon test). In the mutation analysis study, the number of detected CTC per patient was low (median for lung, 4.5; median for breast, 5.5); however, it was easy to detect EGFR and PIK3CA mutations in the CTC of 2 lung and 1 breast cancer patient, respectively, using a commercially available kit. Our system is more sensitive than CellSearch in CTC enumeration of various cancers and is also capable of detecting EGFR and PIK3CA mutations in the CTC of lung and breast cancer patients, respectively.

  18. Calculation of transonic flows using an extended integral equation method

    NASA Technical Reports Server (NTRS)

    Nixon, D.

    1976-01-01

    An extended integral equation method for transonic flows is developed. In the extended integral equation method velocities in the flow field are calculated in addition to values on the aerofoil surface, in contrast with the less accurate 'standard' integral equation method in which only surface velocities are calculated. The results obtained for aerofoils in subcritical flow and in supercritical flow when shock waves are present compare satisfactorily with the results of recent finite difference methods.

  19. Method and Apparatus for Measuring Fluid Flow

    NASA Technical Reports Server (NTRS)

    Arndt, G. Dickey (Inventor); Nguyen, Thanh X. (Inventor); Carl, James R. (Inventor)

    1997-01-01

    Method and apparatus for making measurements on fluids related to their complex permeability are disclosed. A microwave probe is provided for exposure to the fluids. The probe can be non-intrusive or can also be positioned at the location where measurements are to be made. The impedance of the probe is determined. in part. by the complex dielectric constant of the fluids at the probe. A radio frequency signal is transmitted to the probe and the reflected signal is phase and amplitude detected at a rapid rate for the purpose of identifying the fluids. Multiple probes may be selectively positioned to monitor the behavior of the fluids including their flow rate. Fluids may be identified as between two or more different fluids as well as multiple phases of the same fluid based on differences between their complex permittivities.

  20. Nuclear reactor flow control method and apparatus

    DOEpatents

    Church, John P.

    1993-01-01

    Method and apparatus for improving coolant flow in a nuclear reactor during accident as well as nominal conditions. The reactor has a plurality of fuel elements in sleeves and a plenum above the fuel and through which the sleeves penetrate. Holes are provided in the sleeve so that coolant from the plenum can enter the sleeve and cool the fuel. The number and size of the holes are varied from sleeve to sleeve with the number and size of holes being greater for sleeves toward the center of the core and less for sleeves toward the periphery of the core. Preferably the holes are all the same diameter and arranged in rows and columns, the rows starting from the bottom of every sleeve and fewer rows in peripheral sleeves and more rows in the central sleeves.

  1. Nuclear reactor flow control method and apparatus

    DOEpatents

    Church, J.P.

    1993-03-30

    Method and apparatus for improving coolant flow in a nuclear reactor during accident as well as nominal conditions. The reactor has a plurality of fuel elements in sleeves and a plenum above the fuel and through which the sleeves penetrate. Holes are provided in the sleeve so that coolant from the plenum can enter the sleeve and cool the fuel. The number and size of the holes are varied from sleeve to sleeve with the number and size of holes being greater for sleeves toward the center of the core and less for sleeves toward the periphery of the core. Preferably the holes are all the same diameter and arranged in rows and columns, the rows starting from the bottom of every sleeve and fewer rows in peripheral sleeves and more rows in the central sleeves.

  2. Method and device for measuring fluid flow

    DOEpatents

    Atherton, Richard; Marinkovich, Phillip S.; Spadaro, Peter R.; Stout, J. Wilson

    1976-11-23

    This invention is a fluid flow measuring device for determining the coolant flow at the entrance to a specific nuclear reactor fuel region. The device comprises a plurality of venturis having the upstream inlet and throat pressure of each respectively manifolded together to provide one static pressure signal for each region monitored. The device provides accurate flow measurement with low pressure losses and uniform entrance and discharge flow distribution.

  3. Method and apparatus for measuring flow velocity using matched filters

    DOEpatents

    Raptis, Apostolos C.

    1983-01-01

    An apparatus and method for measuring the flow velocities of individual phase flow components of a multiphase flow utilizes matched filters. Signals arising from flow noise disturbance are extracted from the flow, at upstream and downstream locations. The signals are processed through pairs of matched filters which are matched to the flow disturbance frequency characteristics of the phase flow component to be measured. The processed signals are then cross-correlated to determine the transit delay time of the phase flow component between sensing positions.

  4. Method and apparatus for measuring flow velocity using matched filters

    DOEpatents

    Raptis, A.C.

    1983-09-06

    An apparatus and method for measuring the flow velocities of individual phase flow components of a multiphase flow utilizes matched filters. Signals arising from flow noise disturbance are extracted from the flow, at upstream and downstream locations. The signals are processed through pairs of matched filters which are matched to the flow disturbance frequency characteristics of the phase flow component to be measured. The processed signals are then cross-correlated to determine the transit delay time of the phase flow component between sensing positions. 8 figs.

  5. Method and apparatus for measuring flow velocity using matched filters

    SciTech Connect

    Raptis, A.C.

    1981-07-17

    An apparatus and method for measuring the flow velocities of individual phase flow components of a multiphase flow is disclosed. Signals arising from flow noise disturbance are extracted from the flow, at upstream and downstream locations. The signals are processed through pairs of matched filters which are matched to the flow disturbance frequency characteristics of the phase flow component to be measured. The processed signals are then cross-correlated to determine the transit delay time of the phase flow component between sensing positions.

  6. Computational methods for internal flows with emphasis on turbomachinery

    NASA Technical Reports Server (NTRS)

    Mcnally, W. D.; Sockol, P. M.

    1981-01-01

    Current computational methods for analyzing flows in turbomachinery and other related internal propulsion components are presented. The methods are divided into two classes. The inviscid methods deal specifically with turbomachinery applications. Viscous methods, deal with generalized duct flows as well as flows in turbomachinery passages. Inviscid methods are categorized into the potential, stream function, and Euler aproaches. Viscous methods are treated in terms of parabolic, partially parabolic, and elliptic procedures. Various grids used in association with these procedures are also discussed.

  7. Microfluidic devices and methods for integrated flow cytometry

    DOEpatents

    Srivastava, Nimisha; Singh, Anup K.

    2011-08-16

    Microfluidic devices and methods for flow cytometry are described. In described examples, various sample handling and preparation steps may be carried out within a same microfluidic device as flow cytometry steps. A combination of imaging and flow cytometry is described. In some examples, spiral microchannels serve as incubation chambers. Examples of automated sample handling and flow cytometry are described.

  8. Method and Apparatus for Measuring Fluid Flow

    NASA Technical Reports Server (NTRS)

    Arndt, G. Dickey (Inventor); Nguyen, Than X. (Inventor); Carl, James R. (Inventor)

    1995-01-01

    The invention is a method and apparatus for monitoring the presence, concentration, and the movement of fluids. It is based on utilizing electromagnetic measurements of the complex permittivity of the fluids for detecting and monitoring the fluid. More particularly the apparatus uses one or more microwave probes which are placed at the locations where the measurements are to be made. A radio frequency signal is transmitted to the probe and the reflected signal is phase and amplitude detected at a rapid rate for the purpose of identifying the fluids, based on their dielectric constant at the probe. The apparatus can be used for multiple purposes including measures of flow rates, turbulence, dispersion, fluid identification, and changes in flow conditions of multiple fluids or multiple states of a single fluid in a flowline or a holding container. The apparatus includes a probe consisting of two electrical conductors separated by an insulator. A radio frequency signal is communicated to the probe and is reflected back from the portion of the probe exposed to the fluid. The radio frequency signal also provides a reference signal. An oscillator generates a second signal which combined with each of the reference signal and the reflected signal to produce signals of lower frequencies to facilitate filtering and amplifying those signals. The two signals are then mixed in a detector to produce an output signal that is representative of the phase and amplitude change caused by the reflection of the signal at the probe exposed to the fluid. The detector may be a dual phase detector that provides two such output signals that are in phase quadrature. A phase shifter may be provided for selectively changing the phase of the reference signal to improve the sensitivity of at least one of the output signals for more accurate readings and/or for calibration purposes. The two outputs that are in quadrature with respect to each other may be simultaneously monitored to account for

  9. Innovative Method of Greatly Reducing Flow Resistance

    NASA Astrophysics Data System (ADS)

    Lin, Weiyi

    2008-11-01

    In this paper, firstly, the aerated pipe flow experiment is introduced. And some experimental research on comparison between different volumes of air entrained is presented. Secondly, the technical characteristics of gravity pipe flow under the action of Torricelli's vacuum, shortly called as GPFUTV are dissertated, including creative and functional design, fundamental principle and the strange energy loss phenomena, etc. Thirdly, an appeal in relation to the experimental research, the applied studies and basic theory research is given. For instance, Reynolds' experiment under GPFUTV condition, the potential for GPFUTV to be developed for deep seawater suction technology and lifting technology for deep ocean mining, flow stability and flow resistance under GPFUTV condition, etc.

  10. Underground stope optimization with network flow method

    NASA Astrophysics Data System (ADS)

    Bai, Xiaoyu; Marcotte, Denis; Simon, Richard

    2013-03-01

    A new algorithm to optimize stope design for the sublevel stoping mining method is described. The model is based on a cylindrical coordinate defined around the initial vertical raise. Geotechnical constraints on hanging wall and footwall slopes are translated as precedence relations between blocks in the cylindrical coordinate system. Two control parameters with clear engineering meaning are defined to further constrain the solution: (a) the maximum distance of a block from the raise and (b) the horizontal width required to bring the farthest block to the raise. The graph obtained is completed by the addition of a source and a sink node allowing to transform the optimization program to a problem of maximum flow over the graph. The (conditional) optimal stope corresponding to the current raise location and height is obtained. The best location and height for the raise are determined by global optimization. The performance of the algorithm is evaluated with three simple synthetic deposits and one real deposit. Comparison is made with the floating stope technique. The results show that the algorithm effectively meets the geotechnical constraints and control parameters, and produce realistic optimal stope for engineering use.

  11. Polynominal Interpolation Methods for Viscous Flow Calculations

    NASA Technical Reports Server (NTRS)

    Rubin, S. G.; Khosla, P. K.

    1976-01-01

    Higher-order collocation procedures resulting in tridiagonal matrix systems are derived from polynomial spline interpolation and by Hermitian (Taylor series) finite-difference discretization. The similarities and special features of these different developments are discussed. The governing systems apply for both uniform and variable meshes. Hybrid schemes resulting from two different polynomial approximations for the first and second derivatives lead to a nonuniform mesh extension of the so-called compact or Pad? difference technique (Hermite 4). A variety of fourth-order methods are described and the Hermitian approach is extended to sixth-order (Hermite 6). The appropriate spline boundary conditions are derived for all procedures. For central finite differences, this leads to a two-point, second-order accurate generalization of the commonly used three-point end-difference formula. Solutions with several spline and Hermite procedures are presented for the boundary layer equations, with and without mass transfer, and for the incompressible viscous flow in a driven cavity. Divergence and nondivergence equations are considered for the cavity. Among the fourth-order techniques, it is shown that spline 4 has the smallest truncation error. The spline 4 procedure generally requires one-quarter the number of mesh points in a given coordinate direction as a central finite-difference calculation of equal accuracy. The Hermite 6 procedure leads to remarkably accurate boundary layer solutions.

  12. Effective methods for cash flow analysis.

    PubMed

    Sylvestre, J; Urbancic, F R

    1994-07-01

    This article discusses techniques that healthcare financial managers can use to interpret and evaluate information from the statement of cash flows for more effective financial decision-making. The use of these techniques as a basis for systematically planning and controlling cash flows has the potential to benefit all healthcare organizations.

  13. A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex-Peptide Monomers by Flow Cytometry.

    PubMed

    Chandran, P Anoop; Heidu, Sonja; Zelba, Henning; Schmid-Horch, Barbara; Rammensee, Hans-Georg; Pascolo, Steve; Gouttefangeas, Cécile

    2017-01-01

    Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage.

  14. A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry

    PubMed Central

    Chandran, P. Anoop; Heidu, Sonja; Zelba, Henning; Schmid-Horch, Barbara; Rammensee, Hans-Georg; Pascolo, Steve; Gouttefangeas, Cécile

    2017-01-01

    Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage. PMID:28228758

  15. PDF methods for turbulent reactive flows

    NASA Technical Reports Server (NTRS)

    Hsu, Andrew T.

    1995-01-01

    Viewgraphs are presented on computation of turbulent combustion, governing equations, closure problem, PDF modeling of turbulent reactive flows, validation cases, current projects, and collaboration with industry and technology transfer.

  16. Methods of similitude in granular avalanche flows

    NASA Astrophysics Data System (ADS)

    Tai, Yih-Chin; Wang, Yongqi; Gray, J. M. N. T.; Hutter, Kolumban

    Snow avalanches are relatively dry and dense granular flows for which the Savage-Hutter (SH) equations have been demonstrated to be an adequate mathematical model. We review these equations and point out for which cases the equations have been tested against laboratory experiments. Since the equations are scale invariant and because agreement with experiments is good, laboratory experiments can be used to test realistic flows. This is detailed in this paper. We demonstrate how shocks are formed when dilatational flow states merge into compacting states and show that shock formation is an essential mechanism in flows against obstructions. We finally apply the theory of similitude to the design of a projected avalanche protection structure of the Schneefernerhaus at the Zugspitze.

  17. Field methods for measuring concentrated flow erosion

    NASA Astrophysics Data System (ADS)

    Castillo, C.; Pérez, R.; James, M. R.; Quinton, J. N.; Taguas, E. V.; Gómez, J. A.

    2012-04-01

    techniques (3D) for measuring erosion from concentrated flow (pole, laser profilemeter, photo-reconstruction and terrestrial LiDAR) The comparison between two- and three-dimensional methods has showed the superiority of the 3D techniques for obtaining accurate cross sectional data. The results from commonly-used 2D methods can be subject to systematic errors in areal cross section that exceed magnitudes of 10 % on average. In particular, the pole simplified method has showed a clear tendency to understimate areas. Laser profilemeter results show that further research on calibrating optical devices for a variety of soil conditions must be carried out to improve its performance. For volume estimations, photo-reconstruction results provided an excellent approximation to terrestrial laser data and demonstrate that this new remote sensing technique has a promising application field in soil erosion studies. 2D approaches involved important errors even over short measurement distances. However, as well as accuracy, the cost and time requirements of a technique must be considered.

  18. Moving and adaptive grid methods for compressible flows

    NASA Technical Reports Server (NTRS)

    Trepanier, Jean-Yves; Camarero, Ricardo

    1995-01-01

    This paper describes adaptive grid methods developed specifically for compressible flow computations. The basic flow solver is a finite-volume implementation of Roe's flux difference splitting scheme or arbitrarily moving unstructured triangular meshes. The grid adaptation is performed according to geometric and flow requirements. Some results are included to illustrate the potential of the methodology.

  19. Method and apparatus for monitoring two-phase flow. [PWR

    DOEpatents

    Sheppard, J.D.; Tong, L.S.

    1975-12-19

    A method and apparatus for monitoring two-phase flow is provided that is particularly related to the monitoring of transient two-phase (liquid-vapor) flow rates such as may occur during a pressurized water reactor core blow-down. The present invention essentially comprises the use of flanged wire screens or similar devices, such as perforated plates, to produce certain desirable effects in the flow regime for monitoring purposes. One desirable effect is a measurable and reproducible pressure drop across the screen. The pressure drop can be characterized for various known flow rates and then used to monitor nonhomogeneous flow regimes. Another useful effect of the use of screens or plates in nonhomogeneous flow is that such apparatus tends to create a uniformly dispersed flow regime in the immediate downstream vicinity. This is a desirable effect because it usually increases the accuracy of flow rate measurements determined by conventional methods.

  20. Microfluidic flow-flash: method for investigating protein dynamics.

    PubMed

    Toepke, Michael W; Brewer, Scott H; Vu, Dung M; Rector, Kirk D; Morgan, Joel E; Gennis, Robert B; Kenis, Paul J A; Dyer, R Brian

    2007-01-01

    We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design utilizes three-dimensional sheath flow to reduce sample dispersion and minimize sample consumption. The ability to alter the flow rates in the microfluidic flow cells allows a variety of time scales to be studied with submillisecond time resolution. The imaging detection system can be coupled with several spectroscopic probes including fluorescence and UV/visible absorbance spectroscopy. Here, we utilize the microfluidic flow-flash method to probe the kinetics of CO recombination or O2 binding to myoglobin after the laser-induced photolysis of CO from myoglobin by UV/visible absorbance spectral imaging.

  1. Noninvasive method of estimating human newborn regional cerebral blood flow

    SciTech Connect

    Younkin, D.P.; Reivich, M.; Jaggi, J.; Obrist, W.; Delivoria-Papadopoulos, M.

    1982-12-01

    A noninvasive method of estimating regional cerebral blood flow (rCBF) in premature and full-term babies has been developed. Based on a modification of the /sup 133/Xe inhalation rCBF technique, this method uses eight extracranial NaI scintillation detectors and an i.v. bolus injection of /sup 133/Xe (approximately 0.5 mCi/kg). Arterial xenon concentration was estimated with an external chest detector. Cerebral blood flow was measured in 15 healthy, neurologically normal premature infants. Using Obrist's method of two-compartment analysis, normal values were calculated for flow in both compartments, relative weight and fractional flow in the first compartment (gray matter), initial slope of gray matter blood flow, mean cerebral blood flow, and initial slope index of mean cerebral blood flow. The application of this technique to newborns, its relative advantages, and its potential uses are discussed.

  2. A method of determining combustion gas flow

    NASA Technical Reports Server (NTRS)

    Bon Tempi, P. J.

    1968-01-01

    Zirconium oxide coating enables the determination of hot gas flow patterns on liquid rocket injector face and baffle surfaces to indicate modifications that will increase performance and improve combustion stability. The coating withstands combustion temperatures and due to the coarse surface and coloring of the coating, shows the hot gas patterns.

  3. APPROXIMATE MULTIPHASE FLOW MODELING BY CHARACTERISTIC METHODS

    EPA Science Inventory

    The flow of petroleum hydrocarbons, organic solvents and other liquids that are immiscible with water presents the nation with some of the most difficult subsurface remediation problems. One aspect of contaminant transport associated releases of such liquids is the transport as a...

  4. APPROXIMATE MULTIPHASE FLOW MODELING BY CHARACTERISTIC METHODS

    EPA Science Inventory

    The flow of petroleum hydrocarbons, organic solvents and other liquids that are immiscible with water presents the nation with some of the most difficult subsurface remediation problems. One aspect of contaminant transport associated releases of such liquids is the transport as a...

  5. Impulse-based methods for fluid flow

    SciTech Connect

    Cortez, Ricardo

    1995-05-01

    A Lagrangian numerical method based on impulse variables is analyzed. A relation between impulse vectors and vortex dipoles with a prescribed dipole moment is presented. This relation is used to adapt the high-accuracy cutoff functions of vortex methods for use in impulse-based methods. A source of error in the long-time implementation of the impulse method is explained and two techniques for avoiding this error are presented. An application of impulse methods to the motion of a fluid surrounded by an elastic membrane is presented.

  6. Ultrasonic fluid flow measurement method and apparatus

    DOEpatents

    Kronberg, J.W.

    1993-10-12

    An apparatus for measuring the flow of a fluid in a pipe using ultrasonic waves. The apparatus comprises an ultrasonic generator, a lens for focusing the sound energy produced by the generator, and means for directing the focused energy into the side of the pipe through an opening and in a direction close to parallel to the long axis of the pipe. A cone carries the sound energy to the lens from the generator. Depending on the choice of materials, there may be a quarter-wave, acoustic impedance matching section between the generator and the cone to reduce the reflections of energy at the cone boundary. The lens material has an acoustic impedance similar to that of the cone material but a different sonic velocity so that the lens can converge the sound waves in the fluid. A transition section between the lens and the fluid helps to couple the energy to the fluid and assures it is directed as close to parallel to the fluid flow direction as possible. 3 figures.

  7. Ultrasonic fluid flow measurement method and apparatus

    DOEpatents

    Kronberg, James W.

    1993-01-01

    An apparatus for measuring the flow of a fluid in a pipe using ultrasonic waves. The apparatus comprises an ultrasonic generator, a lens for focusing the sound energy produced by the generator, and means for directing the focused energy into the side of the pipe through an opening and in a direction close to parallel to the long axis of the pipe. A cone carries the sound energy to the lens from the generator. Depending on the choice of materials, there may be a quarter-wave, acoustic impedance matching section between the generator and the cone to reduce the reflections of energy at the cone boundary. The lens material has an acoustic impedance similar to that of the cone material but a different sonic velocity so that the lens can converge the sound waves in the fluid. A transition section between the lens and the fluid helps to couple the energy to the fluid and assures it is directed as close to parallel to the fluid flow direction as possible.

  8. Ultrasonic fluid flow measurement method and apparatus

    SciTech Connect

    Kronberg, J.W.

    1992-12-31

    This invention is comprised of an apparatus for measuring the flow of a fluid in a pipe using ultrasonic waves. The apparatus comprises an ultrasonic generator, a lens for focusing the sound energy produced by the generator, and means for directing the focused energy into the side of the pipe through an opening and in a direction close to parallel to the long axis of the pipe. A cone carries the sound energy to the lens from the generator. Depending on the choice of materials, there may be a quarter-wave, acoustic impedance matching section between the generator and the cone to reduce the reflections of energy at the cone boundary. The lens material has an acoustic impedance similar to that of the cone material but a different sonic velocity so that the lens can converge the sound waves in the fluid. A transition section between the lens and the fluid helps to couple the energy to the fluid and assures it is directed as close to parallel to the fluid flow direction as possible.

  9. Ultrasonic 3-D Vector Flow Method for Quantitative In Vivo Peak Velocity and Flow Rate Estimation.

    PubMed

    Holbek, Simon; Ewertsen, Caroline; Bouzari, Hamed; Pihl, Michael Johannes; Hansen, Kristoffer Lindskov; Stuart, Matthias Bo; Thomsen, Carsten; Nielsen, Michael Bachmann; Jensen, Jorgen Arendt

    2017-03-01

    Current clinical ultrasound (US) systems are limited to show blood flow movement in either 1-D or 2-D. In this paper, a method for estimating 3-D vector velocities in a plane using the transverse oscillation method, a 32×32 element matrix array, and the experimental US scanner SARUS is presented. The aim of this paper is to estimate precise flow rates and peak velocities derived from 3-D vector flow estimates. The emission sequence provides 3-D vector flow estimates at up to 1.145 frames/s in a plane, and was used to estimate 3-D vector flow in a cross-sectional image plane. The method is validated in two phantom studies, where flow rates are measured in a flow-rig, providing a constant parabolic flow, and in a straight-vessel phantom ( ∅=8 mm) connected to a flow pump capable of generating time varying waveforms. Flow rates are estimated to be 82.1 ± 2.8 L/min in the flow-rig compared with the expected 79.8 L/min, and to 2.68 ± 0.04 mL/stroke in the pulsating environment compared with the expected 2.57 ± 0.08 mL/stroke. Flow rates estimated in the common carotid artery of a healthy volunteer are compared with magnetic resonance imaging (MRI) measured flow rates using a 1-D through-plane velocity sequence. Mean flow rates were 333 ± 31 mL/min for the presented method and 346 ± 2 mL/min for the MRI measurements.

  10. Rapid flow cytometric measurement of protein inclusions and nuclear trafficking

    PubMed Central

    Whiten, D. R.; San Gil, R.; McAlary, L.; Yerbury, J. J.; Ecroyd, H.; Wilson, M. R.

    2016-01-01

    Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules. PMID:27516358

  11. Fourier time spectral method for subsonic and transonic flows

    NASA Astrophysics Data System (ADS)

    Zhan, Lei; Liu, Feng; Papamoschou, Dimitri

    2016-06-01

    The time accuracy of the exponentially accurate Fourier time spectral method (TSM) is examined and compared with a conventional 2nd-order backward difference formula (BDF) method for periodic unsteady flows. In particular, detailed error analysis based on numerical computations is performed on the accuracy of resolving the local pressure coefficient and global integrated force coefficients for smooth subsonic and non-smooth transonic flows with moving shock waves on a pitching airfoil. For smooth subsonic flows, the Fourier TSM method offers a significant accuracy advantage over the BDF method for the prediction of both the local pressure coefficient and integrated force coefficients. For transonic flows where the motion of the discontinuous shock wave contributes significant higher-order harmonic contents to the local pressure fluctuations, a sufficient number of modes must be included before the Fourier TSM provides an advantage over the BDF method. The Fourier TSM, however, still offers better accuracy than the BDF method for integrated force coefficients even for transonic flows. A problem of non-symmetric solutions for symmetric periodic flows due to the use of odd numbers of intervals is uncovered and analyzed. A frequency-searching method is proposed for problems where the frequency is not known a priori. The method is tested on the vortex shedding problem of the flow over a circular cylinder.

  12. Method and apparatus for coal analysis and flow measurement

    SciTech Connect

    Rollwitz, W.L.; King, J.D.

    1985-07-23

    A flow meter apparatus and method for measuring the flow, composition and heat content of coal is set forth. In the preferred and illustrated embodiment, the free or unpaired electron population of flowing coal is measured by electron magnetic resonance (EMR); the hydrogen nucleii population is measured by nuclear magnetic resonance (NMR). By calibration utilizing a standard specimen for a given type of coal, a profile for various types of coal can be obtained wherein measurement data is converted into an indication of the heat content typically measured in BTU per pound. This enables provision of a volumetric flow meter, a flow meter indicating flow in calorie content. This device enables integration to provide total heat content flow. Other variables describing the coal can be obtained.

  13. Dye visualization--a method for investigating biomechanical flows.

    PubMed

    Leweke, Thomas

    2012-09-01

    Flow visualization using dye is an inexpensive and easy-to-implement experimental technique. It can be used for a rapid qualitative assessment of fluid flows in configurations relevant to biomedical or biotechnological applications, which often involve small spatial dimensions and flow velocities (low Reynolds numbers). This paper gives an overview of the practical aspects related to dye visualization in liquids (dyes, introduction of dye into the flow, illumination), and discusses the information that can be obtained by this method, which includes the distribution of coherent structures/ vortices, the location of recirculation zones, and certain characteristic spatial and temporal scales. Visualization results for three examples of generic flows related to biomechanical applications are presented: the flow behind a contraction in a pipe (stenosis), the wake of a particle moving along a wall, and the flow inside a lid-driven mixing vessel (bioreactor).

  14. Polynomial interpolation methods for viscous flow calculations

    NASA Technical Reports Server (NTRS)

    Rubin, S. G.; Khosla, P. K.

    1977-01-01

    Higher-order collocation procedures which result in block-tridiagonal matrix systems are derived from (1) Taylor series expansions and from (2) polynomial interpolation, and the relationships between the two formulations, called respectively Hermite and spline collocation, are investigated. A Hermite block-tridiagonal system for a nonuniform mesh is derived, and the Hermite approach is extended in order to develop a variable-mesh sixth-order block-tridiagonal procedure. It is shown that all results obtained by Hermite development can be recovered by appropriate spline polynomial interpolation. The additional boundary conditions required for these higher-order procedures are also given. Comparative solutions using second-order accurate finite difference and spline and Hermite formulations are presented for the boundary layer on a flat plate, boundary layers with uniform and variable mass transfer, and the viscous incompressible Navier-Stokes equations describing flow in a driven cavity.

  15. Spectral multigrid methods with applications to transonic potential flow

    NASA Technical Reports Server (NTRS)

    Streett, C. L.; Zang, T. A.; Hussaini, M. Y.

    1983-01-01

    Spectral multigrid methods are demonstrated to be a competitive technique for solving the transonic potential flow equation. The spectral discretization, the relaxation scheme, and the multigrid techniques are described in detail. Significant departures from current approaches are first illustrated on several linear problems. The principal applications and examples, however, are for compressible potential flow. These examples include the relatively challenging case of supercritical flow over a lifting airfoil.

  16. Adaptive implicit-explicit methods for flow problems

    NASA Technical Reports Server (NTRS)

    Liou, J.; Tezduyar, T. E.

    1989-01-01

    Studies on the adaptive implicit-explicit (AIE) method for large-scale problems are presented. The AIE method is based on dynamic grouping of the elements into the implicit and explicit subsets as dictated by the element level stability and accuracy considerations. The method has been applied to various flow problems including the incompressible flow and convection-diffusion problems, and it has been demonstrated that substantial savings in CPU time and memory can be achieved.

  17. Advanced surface paneling method for subsonic and supersonic flow

    NASA Technical Reports Server (NTRS)

    Erickson, L. L.; Johnson, F. T.; Ehlers, F. E.

    1976-01-01

    Numerical results illustrating the capabilities of an advanced aerodynamic surface paneling method are presented. The method is applicable to both subsonic and supersonic flow, as represented by linearized potential flow theory. The method is based on linearly varying sources and quadratically varying doublets which are distributed over flat or curved panels. These panels are applied to the true surface geometry of arbitrarily shaped three dimensional aerodynamic configurations.

  18. Vortex method for blood flow through heart valves

    SciTech Connect

    McCracken, M.F.; Peskin, C.S.

    1980-04-01

    A combination vortex-grid method for solving the two-dimensional, incompressible Navier--Stokes equations in regions with complicated internal, elastic boundaries is presented. The authors believe the method to be applicable to the case of at least moderately high Reynolds number flow. The method is applied to the study of blood flow through the mammalian mitral valve. Previous work of Peskin is extended and the conjecture that the behavior of mammalian heart valves is independent of Reynolds number is supported.

  19. Method analyzes pressure for short flow times

    SciTech Connect

    Soliman, M. ); Petak, K. )

    1990-04-30

    Pressure build-up after short producing times and pressure surges can be analyzed. The method employs the derivative plot and does not require prior knowledge of initial reservoir pressure that may be calculated using a Cartesian plot. Field use of the new technique has proven its superiority to previously used methods, and has shown the necessity of using highly accurate data to achieve desired results.

  20. A Reconstruction Method of Blood Flow Velocity in Left Ventricle Using Color Flow Ultrasound

    PubMed Central

    Jang, Jaeseong; Ahn, Chi Young; Jeon, Kiwan; Heo, Jung; Lee, DongHak; Choi, Jung-il

    2015-01-01

    Vortex flow imaging is a relatively new medical imaging method for the dynamic visualization of intracardiac blood flow, a potentially useful index of cardiac dysfunction. A reconstruction method is proposed here to quantify the distribution of blood flow velocity fields inside the left ventricle from color flow images compiled from ultrasound measurements. In this paper, a 2D incompressible Navier-Stokes equation with a mass source term is proposed to utilize the measurable color flow ultrasound data in a plane along with the moving boundary condition. The proposed model reflects out-of-plane blood flows on the imaging plane through the mass source term. The boundary conditions to solve the system of equations are derived from the dimensions of the ventricle extracted from 2D echocardiography data. The performance of the proposed method is evaluated numerically using synthetic flow data acquired from simulating left ventricle flows. The numerical simulations show the feasibility and potential usefulness of the proposed method of reconstructing the intracardiac flow fields. Of particular note is the finding that the mass source term in the proposed model improves the reconstruction performance. PMID:26078773

  1. A Reconstruction Method of Blood Flow Velocity in Left Ventricle Using Color Flow Ultrasound.

    PubMed

    Jang, Jaeseong; Ahn, Chi Young; Jeon, Kiwan; Heo, Jung; Lee, DongHak; Joo, Chulmin; Choi, Jung-il; Seo, Jin Keun

    2015-01-01

    Vortex flow imaging is a relatively new medical imaging method for the dynamic visualization of intracardiac blood flow, a potentially useful index of cardiac dysfunction. A reconstruction method is proposed here to quantify the distribution of blood flow velocity fields inside the left ventricle from color flow images compiled from ultrasound measurements. In this paper, a 2D incompressible Navier-Stokes equation with a mass source term is proposed to utilize the measurable color flow ultrasound data in a plane along with the moving boundary condition. The proposed model reflects out-of-plane blood flows on the imaging plane through the mass source term. The boundary conditions to solve the system of equations are derived from the dimensions of the ventricle extracted from 2D echocardiography data. The performance of the proposed method is evaluated numerically using synthetic flow data acquired from simulating left ventricle flows. The numerical simulations show the feasibility and potential usefulness of the proposed method of reconstructing the intracardiac flow fields. Of particular note is the finding that the mass source term in the proposed model improves the reconstruction performance.

  2. Lattice Boltzmann Method for Two-Dimensional Unsteady Incompressible Flow

    NASA Astrophysics Data System (ADS)

    Mužík, Juraj

    2016-12-01

    A Lattice Boltzmann method is used to analyse incompressible fluid flow in a two-dimensional cavity and flow in the channel past cylindrical obstacle. The method solves the Boltzmann's transport equation using simple computational grid - lattice. With the proper choice of the collision operator, the Boltzmann's equation can be converted into incompressible Navier-Stokes equation. Lid-driven cavity benchmark case for various Reynolds numbers and flow past cylinder is presented in the article. The method produces stable solutions with results comparable to those in literature and is very easy to implement.

  3. Cross-flow electrofilter and method

    DOEpatents

    Gidaspow, Dimitri; Lee, Chang H.; Wasan, Darsh T.

    1980-01-01

    A filter for clarifying carbonaceous liquids containing finely divided solid particles of, for instance, unreacted coal, ash and other solids discharged from a coal liquefaction process is presented. The filter includes two passageways separated by a porous filter medium. In one preferred embodiment the filter medium is of tubular shape to form the first passageway and is enclosed within an outer housing to form the second passageway within the annulus. An electrode disposed in the first passageway, for instance along the tube axis, is connected to a source of high voltage for establishing an electric field between the electrode and the filter medium. Slurry feed flows through the first passageway tangentially to the surfaces of the filter medium and the electrode. Particles from the feed slurry are attracted to the electrode within the first passageway to prevent plugging of the porous filter medium while carbonaceous liquid filters into the second passageway for withdrawal. Concentrated slurry is discharged from the first passageway at an end opposite to the feed slurry inlet. Means are also provided for the addition of diluent and a surfactant into the slurry to control relative permittivity and the electrophoretic mobility of the particles.

  4. Flow visualization for Lagrangian particle methods

    SciTech Connect

    Glass, M.W.; Kempka, S.N.

    1994-05-01

    In particle methods, each particle represents a finite region over which there is a distribution of the field quantity of interest. The field value at any point is calculated by summing the distribution functions for all the particles. This summation procedure does not require the use of any connectivities to generate continuous fields. Various AVS modules and networks have been developed that enable us to visualize the results from particle methods. This will be demonstrated by visualizing a numerical simulation of a rising, chaotic bubble. In this fluid dynamics simulation, each particle represents a region with a specified vorticity distribution.

  5. A conservative interface method for compressible flows

    NASA Astrophysics Data System (ADS)

    Hu, X. Y.; Khoo, B. C.; Adams, N. A.; Huang, F. L.

    2006-12-01

    In this work, we present a conservative interface method for both multi-fluid and complex boundary problems, in which the standard finite volume scheme on Cartesian grids is modified by considering computational cells being cut by interface. While the discretized governing equations are updated conservatively, the method treats the topological changes naturally by combining interface description and geometric operations with a level set technique. Extensive tests in 1D are carried out, and 2D examples suggest that the present scheme is able to handle multi-fluid and complex (static or moving) boundary problems in a straightforward way with good robustness and accuracy.

  6. PDF methods for combustion in high-speed turbulent flows

    NASA Technical Reports Server (NTRS)

    Pope, Stephen B.

    1995-01-01

    This report describes the research performed during the second year of this three-year project. The ultimate objective of the project is extend the applicability of probability density function (pdf) methods from incompressible to compressible turbulent reactive flows. As described in subsequent sections, progress has been made on: (1) formulation and modelling of pdf equations for compressible turbulence, in both homogeneous and inhomogeneous inert flows; and (2) implementation of the compressible model in various flow configurations, namely decaying isotropic turbulence, homogeneous shear flow and plane mixing layer.

  7. Flow cytometric allergy diagnosis: basophil activation techniques.

    PubMed

    Bridts, Chris H; Sabato, Vito; Mertens, Christel; Hagendorens, Margo M; De Clerck, Luc S; Ebo, Didier G

    2014-01-01

    The basis of flow cytometric allergy diagnosis is quantification of changes in expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332-339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies (Ebo et al., Cytometry B Clin Cytom 74: 201-210, 2008). Here, we describe two flow cytometry-based protocols which allow detection of surface marker activation (Method 1) and changes in intragranular histamine (Method 2), both reflecting different facets of basophil activation.

  8. The art and science of flow control - case studies using flow visualization methods

    NASA Astrophysics Data System (ADS)

    Alvi, F. S.; Cattafesta, L. N., III

    2010-04-01

    Active flow control (AFC) has been the focus of significant research in the last decade. This is mainly due to the potentially substantial benefits it affords. AFC applications range from the subsonic to the supersonic (and beyond) regime for both internal and external flows. These applications are wide and varied, such as controlling flow transition and separation over various external components of the aircraft to active management of separation and flow distortion in engine components and over turbine and compressor blades. High-speed AFC applications include control of flow oscillations in cavity flows, supersonic jet screech, impinging jets, and jet-noise control. In this paper we review some of our recent applications of AFC through a number of case studies that illustrate the typical benefits as well as limitations of present AFC methods. The case studies include subsonic and supersonic canonical flowfields such as separation control over airfoils, control of supersonic cavity flows and impinging jets. In addition, properties of zero-net mass-flux (ZNMF) actuators are also discussed as they represent one of the most widely studied actuators used for AFC. In keeping with the theme of this special issue, the flowfield properties and their response to actuation are examined through the use of various qualitative and quantitative flow visualization methods, such as smoke, shadowgraph, schlieren, planar-laser scattering, and Particle image velocimetry (PIV). The results presented here clearly illustrate the merits of using flow visualization to gain significant insight into the flow and its response to AFC.

  9. Red blood cell tracking using optical flow methods.

    PubMed

    Guo, Dongmin; van de Ven, Anne L; Zhou, Xiaobo

    2014-05-01

    The investigation of microcirculation is an important task in biomedical and physiological research because the microcirculation information, such as flow velocity and vessel density, is critical to monitor human conditions and develop effective therapies of some diseases. As one of the tasks of the microcirculation study, red blood cell (RBC) tracking presents an effective approach to estimate some parameters in microcirculation. The common method for RBC tracking is based on spatiotemporal image analysis, which requires the image to have high qualification and cells should have fixed velocity. Besides, for in vivo cell tracking, cells may disappear in some frames, image series may have spatial and temporal distortions, and vessel distribution can be complex, which increase the difficulties of RBC tracking. In this paper, we propose an optical flow method to track RBCs. It attempts to describe the local motion for each visible point in the frames using a local displacement vector field. We utilize it to calculate the displacement of a cell in two adjacent frames. Additionally, another optical flow-based method, scale invariant feature transform (SIFT) flow, is also presented. The experimental results show that optical flow is quite robust to the case where the velocity of cell is unstable, while SIFT flow works well when there is a large displacement of the cell between two adjacent frames. Our proposed methods outperform other methods when doing in vivo cell tracking, which can be used to estimate the blood flow directly and help to evaluate other parameters in microcirculation.

  10. Developments in flow visualization methods for flight research

    NASA Technical Reports Server (NTRS)

    Holmes, Bruce J.; Obara, Clifford J.; Manuel, Gregory S.; Lee, Cynthia C.

    1990-01-01

    With the introduction of modern airplanes utilizing laminar flow, flow visualization has become an important diagnostic tool in determining aerodynamic characteristics such as surface flow direction and boundary-layer state. A refinement of the sublimating chemical technique has been developed to define both the boundary-layer transition location and the transition mode. In response to the need for flow visualization at subsonic and transonic speeds and altitudes above 20,000 feet, the liquid crystal technique has been developed. A third flow visualization technique that has been used is infrared imaging, which offers non-intrusive testing over a wide range of test conditions. A review of these flow visualization methods and recent flight results is presented for a variety of modern aircraft and flight conditions.

  11. Developments in flow visualization methods for flight research

    NASA Technical Reports Server (NTRS)

    Holmes, Bruce J.; Obara, Clifford J.; Manuel, Gregory S.; Lee, Cynthia C.

    1990-01-01

    With the introduction of modern airplanes utilizing laminar flow, flow visualization has become an important diagnostic tool in determining aerodynamic characteristics such as surface flow direction and boundary-layer state. A refinement of the sublimating chemical technique has been developed to define both the boundary-layer transition location and the transition mode. In response to the need for flow visualization at subsonic and transonic speeds and altitudes above 20,000 feet, the liquid crystal technique has been developed. A third flow visualization technique that has been used is infrared imaging, which offers non-intrusive testing over a wide range of test conditions. A review of these flow visualization methods and recent flight results is presented for a variety of modern aircraft and flight conditions.

  12. A flux monitoring method for easy and accurate flow rate measurement in pressure-driven flows.

    PubMed

    Siria, Alessandro; Biance, Anne-Laure; Ybert, Christophe; Bocquet, Lydéric

    2012-03-07

    We propose a low-cost and versatile method to measure flow rate in microfluidic channels under pressure-driven flows, thereby providing a simple characterization of the hydrodynamic permeability of the system. The technique is inspired by the current monitoring method usually employed to characterize electro-osmotic flows, and makes use of the measurement of the time-dependent electric resistance inside the channel associated with a moving salt front. We have successfully tested the method in a micrometer-size channel, as well as in a complex microfluidic channel with a varying cross-section, demonstrating its ability in detecting internal shape variations.

  13. DEMONSTRATION BULLETIN: COLLOID POLISHING FILTER METHOD - FILTER FLOW TECHNOLOGY, INC.

    EPA Science Inventory

    The Filter Flow Technology, Inc. (FFT) Colloid Polishing Filter Method (CPFM) was tested as a transportable, trailer mounted, system that uses sorption and chemical complexing phenomena to remove heavy metals and nontritium radionuclides from water. Contaminated waters can be pro...

  14. Pressure algorithm for elliptic flow calculations with the PDF method

    NASA Technical Reports Server (NTRS)

    Anand, M. S.; Pope, S. B.; Mongia, H. C.

    1991-01-01

    An algorithm to determine the mean pressure field for elliptic flow calculations with the probability density function (PDF) method is developed and applied. The PDF method is a most promising approach for the computation of turbulent reacting flows. Previous computations of elliptic flows with the method were in conjunction with conventional finite volume based calculations that provided the mean pressure field. The algorithm developed and described here permits the mean pressure field to be determined within the PDF calculations. The PDF method incorporating the pressure algorithm is applied to the flow past a backward-facing step. The results are in good agreement with data for the reattachment length, mean velocities, and turbulence quantities including triple correlations.

  15. DEMONSTRATION BULLETIN: COLLOID POLISHING FILTER METHOD - FILTER FLOW TECHNOLOGY, INC.

    EPA Science Inventory

    The Filter Flow Technology, Inc. (FFT) Colloid Polishing Filter Method (CPFM) was tested as a transportable, trailer mounted, system that uses sorption and chemical complexing phenomena to remove heavy metals and nontritium radionuclides from water. Contaminated waters can be pro...

  16. Characterization of peak flow events with local singularity method

    NASA Astrophysics Data System (ADS)

    Cheng, Q.; Li, L.; Wang, L.

    2009-07-01

    Three methods, return period, power-law frequency plot (concentration-area) and local singularity index, are introduced in the paper for characterizing peak flow events from river flow data for the past 100 years from 1900 to 2000 recorded at 25 selected gauging stations on rivers in the Oak Ridges Moraine (ORM) area, Canada. First a traditional method, return period, was applied to the maximum annual river flow data. Whereas the Pearson III distribution generally fits the values, a power-law frequency plot (C-A) on the basis of self-similarity principle provides an effective mean for distinguishing "extremely" large flow events from the regular flow events. While the latter show a power-law distribution, about 10 large flow events manifest departure from the power-law distribution and these flow events can be classified into a separate group most of which are related to flood events. It is shown that the relation between the average water releases over a time period after flow peak and the time duration may follow a power-law distribution. The exponent of the power-law or singularity index estimated from this power-law relation may be used to characterize non-linearity of peak flow recessions. Viewing large peak flow events or floods as singular processes can anticipate the application of power-law models not only for characterizing the frequency distribution of peak flow events, for example, power-law relation between the number and size of floods, but also for describing local singularity of processes such as power-law relation between the amount of water released versus releasing time. With the introduction and validation of singularity of peak flow events, alternative power-law models can be used to depict the recession property as well as other types of non-linear properties.

  17. Flow measurement on an oscillating pipe flow near the entrance using the UVP method

    NASA Astrophysics Data System (ADS)

    Yamanaka, G.; Kikura, H.; Takeda, Y.; Aritomi, M.

    The authors have carried out a study to investigate and clarify the characteristics of purely oscillating pipe flows over the developing region. The main objective of this study is to establish the method of time-dependent velocity profiles obtained by the ultrasonic velocity profile (UVP) measurement method. First, the relationship between the test fluids and the microparticles, as reflectors of ultrasonic pulses, was investigated. In addition, the relationship between the sound speeds of the test fluids and the wall materials was studied. Second, the UVP was used to obtain the instantaneous velocity profiles in oscillating pipe flows, and the developing characteristics of the flows were analyzed. Finally, the ``entrance length'' (by analogy with a unidirectional pipe flow) required for oscillating pipe flows was analyzed by examining the amplitude of the harmonic spectral components of the oscillating frequency. A fast Fourier transform (FFT) is proposed as the applicable method to estimate the ``entrance length''. From the Fourier transform of the velocity on the centerline, nonlinear oscillation of fluid occurs in the ``entrance length'' of the oscillating flows, and the viscous dissipation of the higher-order velocity harmoncis determines the entrance region. The ``entrance length'' can be obtained from the dissipation length of the third-order harmonic. These results prove that the UVP method is highly applicable to carry out the flow measurement in the ``entrance length'' of oscillating pipe flow.

  18. Flow measurement on oscillating pipe flow near the entrance using the UVP method

    NASA Astrophysics Data System (ADS)

    Yamanaka, G.; Kikura, H.; Takeda, Y.; Aritomi, M.

    2003-02-01

    The authors have carried out a study to investigate and clarify the characteristics of purely oscillating pipe flows over the developing region. The main objective of this study is to establish the method of time-dependent velocity profiles obtained by the ultrasonic velocity profile (UVP) measurement method. First, the relationship between the test fluids and the micro-particles, as reflectors of ultrasonic pulses, was investigated, and also the relationship between the sound speeds of the test fluids and wall materials was studied. Second, the UVP was used to obtain the instantaneous velocity profiles in oscillating pipe flows, and the developing characteristics of the flows were analyzed. Finally, the "entrance length" (analogy with a unidirectional pipe flow) required for oscillating pipe flows were analyzed examining the amplitude of the harmonic spectral components of the oscillating frequency. The fast Fourier transform (FFT) is proposed as the applicable method to estimate the "entrance length". From the Fourier transform of the velocity on the centerline, nonlinear oscillation of fluid occurs in the "entrance length" of the oscillating flows, and the viscous dissipation of the higher order velocity harmonics determines the entrance region. The "entrance length" can be obtained from the dissipation length of the third order harmonic. These results prove that the UVP method is highly applicable for carrying out flow measurements in the "entrance length" of the oscillating pipe flow.

  19. Panel-Method Computer Code For Potential Flow

    NASA Technical Reports Server (NTRS)

    Ashby, Dale L.; Dudley, Michael R.; Iguchi, Steven K.

    1992-01-01

    Low-order panel method used to reduce computation time. Panel code PMARC (Panel Method Ames Research Center) numerically simulates flow field around or through complex three-dimensional bodies such as complete aircraft models or wind tunnel. Based on potential-flow theory. Facilitates addition of new features to code and tailoring of code to specific problems and computer-hardware constraints. Written in standard FORTRAN 77.

  20. Packet flow monitoring tool and method

    DOEpatents

    Thiede, David R [Richland, WA

    2009-07-14

    A system and method for converting packet streams into session summaries. Session summaries are a group of packets each having a common source and destination internet protocol (IP) address, and, if present in the packets, common ports. The system first captures packets from a transport layer of a network of computer systems, then decodes the packets captured to determine the destination IP address and the source IP address. The system then identifies packets having common destination IP addresses and source IP addresses, then writes the decoded packets to an allocated memory structure as session summaries in a queue.

  1. Comparison of different secondary flow correction methods for depth-average flow calculations in bends

    NASA Astrophysics Data System (ADS)

    Qin, C. C.

    2014-12-01

    Several secondary flow correction methods had been proposed in literature to account for the 3d flow characteristics of secondary circulation which was often lost in depth-averaged models. In order to evaluate their performances in singular bend, successive reverse curvature bends and natural meander rivers, three different typical secondary flow correction methods were applied to a depth-averaged hydrodynamic model, which were Lien(L), Kalkwijk and Booij(K) and modified Finnie(F) methods. There are obviously limits to the descriptive ability of the three methods in simulating sharply bend channels, especially L method which showed qualitative error. As for successive reverse curvature bends and natural meander rivers, K method gave a better performance than the others. F method increased depth-averaged velocities on the outside of curves, while L method overestimated the whole flow field. By the order of magnitude analysis, we found that L method has done the maximum correction for the secondary flow and the minimum correction for transversal velocities was F method. Those three methods were evaluated by multiple performance criteria. The results of this study can provide some references for some practical applications.

  2. New method to measure coronary velocity and coronary flow reserve.

    PubMed

    Zhang, Z-D; Svendsen, M; Choy, J S; Sinha, A K; Huo, Y; Yoshida, K; Molloi, S; Kassab, G S

    2011-07-01

    Coronary flow reserve (CFR) is an important index of coronary microcirculatory function. The objective of this study was to validate the reproducibility and accuracy of intravascular conductance catheter-based method for measurements of baseline and hyperemic coronary flow velocity (and hence CFR). The absolute coronary blood velocity was determined by measuring the time of transit of a saline injection between two pairs of electrodes (known distance) on a conductance catheter during a routine saline injection without the need for reference flow. In vitro validation was made in the velocity range of 5 to 70 cm/s in reference to the volume collection method. In 10 swine, velocity measurements were compared with those from a flow probe in coronary arteries at different CFR attained by microsphere embolization. In vitro, the mean difference between the proposed method and volume collection was 0.7 ± 1.34 cm/s for steady flow and -0.77 ± 2.22 cm/s for pulsatile flow. The mean difference between duplicate measurements was 0 ± 1.4 cm/s. In in vivo experiments, the flow (product of velocity and lumen cross-sectional area that is also measured by the conductance catheter) was determined in both normal and stenotic vessels and the mean difference between the proposed method and flow probe was -1 ± 12 ml/min (flow ranged from 10 to 130 ml/min). For CFR, the mean difference between the two methods was 0.06 ± 0.28 (range of 1 to 3). Our results demonstrate the reproducibility and accuracy of velocity and CFR measurements with a conductance catheter by use of a standard saline injection. The ability of the combined measurement of coronary lumen area (as previously validated) and current velocity and CFR measurements provides an integrative diagnostic tool for interventional cardiology.

  3. Blood flow quantification using optical flow methods in a body fitted coordinate system

    NASA Astrophysics Data System (ADS)

    Maday, Peter; Brosig, Richard; Endres, Jurgen; Kowarschik, Markus; Navab, Nassir

    2014-03-01

    In this paper a blood flow quantification method that is based on a physically motivated dense 2D flow estimation algorithm is outlined. It yields accurate time varying volumetric flow rate measurements based on digital subtraction angiography (DSA) image sequences, with robustness to significant inter-frame displacements. Time varying volumetric flow rates are estimated for individual non-branching vascular segments based on the estimated 2D flow fields and a 3D vessel segmentation from a 3D Rotational Angiography (3DRA) acquisition. The novelty of the approach lies in the use of a vessel aligned coordinate system for the problem formulation. The coordinate functions are generated using the Schwarz-Christoffel1(SC) map that yields a solution with coordinate lines aligned with the vessel boundaries. The use of vessel aligned coordinates enables the easy and accurate handling of boundary conditions in the irregular domain of a vessel lumen while only requiring slight modifications to the used finite difference approach. Unlike traditional coarse to fine methods we use an anisotropic scaling strategy that enables the estimation of flows with larger inter frame displacements. The evaluation of our method is based on highly realistic synthetic DSA datasets for a number of cases. Ground truth volumetric flow rate values are compared against the measurements and a high degree of fidelity is observed. Performance measures are obtained with varying flow velocities and acquisition rates.

  4. Method of analysis for compressible flow through mixed-flow centrifugal impellers of arbitrary design

    NASA Technical Reports Server (NTRS)

    Hamrick, Joseph T; Ginsburg, Ambrose; Osborn, Walter M

    1952-01-01

    A method is presented for analysis of the compressible flow between the hub and the shroud of mixed-flow impellers of arbitrary design. Axial symmetry was assumed, but the forces in the meridional (hub to shroud) plane, which are derived from tangential pressure gradients, were taken into account. The method was applied to an experimental mixed-flow impeller. The analysis of the flow in the meridional plane of the impeller showed that the rotational forces, the blade curvature, and the hub-shroud profile can introduce severe velocity gradients along the hub and the shroud surfaces. Choked flow at the impeller inlet as determined by the analysis was verified by experimental results.

  5. A semi-probabilistic assessment method for flow slides

    NASA Astrophysics Data System (ADS)

    van den Ham, G.; Mastbergen, D.; de Groot, M.

    2013-12-01

    Flow slides in submerged slopes in non-lithified sandy and silty sediments form a major threat for flood defences along (estuary) coastlines and riverbanks in the Netherlands. Such flow slides may result in failure of levees and structures, eventually leading to flooding of the hinterland. Flow slide is a complex failure mechanism that includes both soil mechanical and hydraulic features. Two important sub-mechanisms are static liquefaction and breach flow. Static liquefaction entails the sudden loss of strength of loosely packed saturated sand or silt resulting in a collapse of the sand body. Breach flow is a more superficial process, involving the upslope retrogression of a local steep part of the slope which generates a turbulent sand-water mixture flow along the sand surface of the under water slope. Both mechanisms need a trigger, e.g. local steepening of the slope by erosion or slip failure. Although a breach flow slide generally takes more time than a liquefaction flow slide, both mechanisms result in a flowing sand-water mixture, that eventually resedimentates under a very gentle slope. Therefore in the analysis of historical flow slides it is often not clear to what extent static soil liquefaction and/or breach flow has played a role. In the current Dutch practice the prediction of levee failure due to flow sliding is based on either simple but conservative empirical rules based on documented historical flow slides in which distinction between mentioned sub-mechanisms is disregarded, or rather complex physical-based models describing mechanisms such as static liquefaction or breach flow. It will be presented how both approaches can be combined into a practical, probabilistic method for assessing dike failure due to flow sliding, accounting for uncertainties of the main influence factors. The method has recently been implemented in the so-called Dike Analysis Module (DAM). DAM is a platform for performing semi-automatic stability analyses on a large number

  6. Method, apparatus and system for controlling fluid flow

    DOEpatents

    McMurtrey, Ryan D.; Ginosar, Daniel M.; Burch, Joesph V.

    2007-10-30

    A system, apparatus and method of controlling the flow of a fluid are provided. In accordance with one embodiment of the present invention, a flow control device includes a valve having a flow path defined therethrough and a valve seat in communication with the flow path with a valve stem disposed in the valve seat. The valve stem and valve seat are cooperatively configured to cause mutual relative linear displacement thereof in response to rotation of the valve stem. A gear member is coupled with the rotary stem and a linear positioning member includes a portion which complementarily engages the gear member. Upon displacement of the linear positioning member along a first axis, the gear member and rotary valve stem are rotated about a second axis and the valve stem and valve seat are mutually linearly displaced to alter the flow of fluid through the valve.

  7. A study of methods to estimate debris flow velocity

    USGS Publications Warehouse

    Prochaska, A.B.; Santi, P.M.; Higgins, J.D.; Cannon, S.H.

    2008-01-01

    Debris flow velocities are commonly back-calculated from superelevation events which require subjective estimates of radii of curvature of bends in the debris flow channel or predicted using flow equations that require the selection of appropriate rheological models and material property inputs. This research investigated difficulties associated with the use of these conventional velocity estimation methods. Radii of curvature estimates were found to vary with the extent of the channel investigated and with the scale of the media used, and back-calculated velocities varied among different investigated locations along a channel. Distinct populations of Bingham properties were found to exist between those measured by laboratory tests and those back-calculated from field data; thus, laboratory-obtained values would not be representative of field-scale debris flow behavior. To avoid these difficulties with conventional methods, a new preliminary velocity estimation method is presented that statistically relates flow velocity to the channel slope and the flow depth. This method presents ranges of reasonable velocity predictions based on 30 previously measured velocities. ?? 2008 Springer-Verlag.

  8. Powder Flow Testing: Judicious Choice of Test Methods.

    PubMed

    Tay, Justin Yong Soon; Liew, Celine Valeria; Heng, Paul Wan Sia

    2016-11-14

    Flow property of pharmaceutical powders can be assessed by various flow testers and test methods. In this study, eight commercially available lactose grades were sourced and tested for angles of repose, tapping studies, shear cell measurements, stirred powder rheometry, and avalanching powder measurements. The relationships between various flow parameters and particle size were analyzed. Deviations from the general trend could be attributed to either the insensitivity of the test or differences in particle shape. The basic flowability energy of the powder rheometer was unable to reconcile the effects of shape and particle size on powder flowability. Avalanche time of the revolving drum powder analyzer and angle of repose exhibited good correlation with each other (r = 0.92) but experienced poor resolution for samples of smaller particle sizes due to powder cohesiveness and the propensity for agglomerative flow. Flow test parameters could be categorized into three broad types, based on their relationship with particle size: (i) linear relationship, (ii) test parameter more sensitive to smaller sized particles, and (iii) test parameter more sensitive to larger sized particles. Choice of test parameters used to represent powder flow should be dependent on the sensitivity of the selected flow test methods to the sample types.

  9. A Semi-Implicit Lattice Method for Simulating Flow

    SciTech Connect

    Rector, David R.; Stewart, Mark L.

    2010-09-20

    We propose a new semi-implicit lattice numerical method for modeling fluid flow that depends only on local primitive variable information (density, pressure, velocity) and not on relaxed upstream distribution function values. This method has the potential for reducing parallel communication and permitting larger time steps compared to the lattice Boltzmann method. The lid-driven cavity is modeled to demonstrate the accuracy of the method.

  10. Fiber optic liquid mass flow sensor and method

    NASA Technical Reports Server (NTRS)

    Korman, Valentin (Inventor); Gregory, Don Allen (Inventor); Wiley, John T. (Inventor); Pedersen, Kevin W. (Inventor)

    2010-01-01

    A method and apparatus are provided for sensing the mass flow rate of a fluid flowing through a pipe. A light beam containing plural individual wavelengths is projected from one side of the pipe across the width of the pipe so as to pass through the fluid under test. Fiber optic couplers located at least two positions on the opposite side of the pipe are used to detect the light beam. A determination is then made of the relative strengths of the light beam for each wavelength at the at least two positions and based at least in part on these relative strengths, the mass flow rate of the fluid is determined.

  11. Assessment of nonequilibrium radiation computation methods for hypersonic flows

    NASA Technical Reports Server (NTRS)

    Sharma, Surendra

    1993-01-01

    The present understanding of shock-layer radiation in the low density regime, as appropriate to hypersonic vehicles, is surveyed. Based on the relative importance of electron excitation and radiation transport, the hypersonic flows are divided into three groups: weakly ionized, moderately ionized, and highly ionized flows. In the light of this division, the existing laboratory and flight data are scrutinized. Finally, an assessment of the nonequilibrium radiation computation methods for the three regimes in hypersonic flows is presented. The assessment is conducted by comparing experimental data against the values predicted by the physical model.

  12. Topography Modeling in Atmospheric Flows Using the Immersed Boundary Method

    NASA Technical Reports Server (NTRS)

    Ackerman, A. S.; Senocak, I.; Mansour, N. N.; Stevens, D. E.

    2004-01-01

    Numerical simulation of flow over complex geometry needs accurate and efficient computational methods. Different techniques are available to handle complex geometry. The unstructured grid and multi-block body-fitted grid techniques have been widely adopted for complex geometry in engineering applications. In atmospheric applications, terrain fitted single grid techniques have found common use. Although these are very effective techniques, their implementation, coupling with the flow algorithm, and efficient parallelization of the complete method are more involved than a Cartesian grid method. The grid generation can be tedious and one needs to pay special attention in numerics to handle skewed cells for conservation purposes. Researchers have long sought for alternative methods to ease the effort involved in simulating flow over complex geometry.

  13. Modeling Electrokinetic Flows by the Smoothed Profile Method

    PubMed Central

    Luo, Xian; Beskok, Ali; Karniadakis, George Em

    2010-01-01

    We propose an efficient modeling method for electrokinetic flows based on the Smoothed Profile Method (SPM) [1–4] and spectral element discretizations. The new method allows for arbitrary differences in the electrical conductivities between the charged surfaces and the the surrounding electrolyte solution. The electrokinetic forces are included into the flow equations so that the Poisson-Boltzmann and electric charge continuity equations are cast into forms suitable for SPM. The method is validated by benchmark problems of electroosmotic flow in straight channels and electrophoresis of charged cylinders. We also present simulation results of electrophoresis of charged microtubules, and show that the simulated electrophoretic mobility and anisotropy agree with the experimental values. PMID:20352076

  14. A Numerical Method for Incompressible Flow with Heat Transfer

    NASA Technical Reports Server (NTRS)

    Sa, Jong-Youb; Kwak, Dochan

    1997-01-01

    A numerical method for the convective heat transfer problem is developed for low speed flow at mild temperatures. A simplified energy equation is added to the incompressible Navier-Stokes formulation by using Boussinesq approximation to account for the buoyancy force. A pseudocompressibility method is used to solve the resulting set of equations for steady-state solutions in conjunction with an approximate factorization scheme. A Neumann-type pressure boundary condition is devised to account for the interaction between pressure and temperature terms, especially near a heated or cooled solid boundary. It is shown that the present method is capable of predicting the temperature field in an incompressible flow.

  15. A time accurate finite volume method for propulsion chamber flows

    NASA Technical Reports Server (NTRS)

    Beddini, R. A.; Ridder, J. P.

    1989-01-01

    An implicit three-dimensional time-accurate method for propulsion chamber flows is proposed which uses line Gauss-Seidel relaxation and multiple axial sweeps for the convergence of each time step. The general time-integration algorithm employed includes such schemes as the Euler implicit method. The results of spatial and temporal accuracy tests reveal that Roe's (1981) flux difference splitting provides excellent tracking of acoustic wave speeds. In comparison with other methods, no low mean flow Mach number convergence limitation or Courant number stabilization restriction is observed.

  16. Applying flow chemistry: methods, materials, and multistep synthesis.

    PubMed

    McQuade, D Tyler; Seeberger, Peter H

    2013-07-05

    The synthesis of complex molecules requires control over both chemical reactivity and reaction conditions. While reactivity drives the majority of chemical discovery, advances in reaction condition control have accelerated method development/discovery. Recent tools include automated synthesizers and flow reactors. In this Synopsis, we describe how flow reactors have enabled chemical advances in our groups in the areas of single-stage reactions, materials synthesis, and multistep reactions. In each section, we detail the lessons learned and propose future directions.

  17. Uncertainty-Based Design Methods for Flow-Structure Interactions

    DTIC Science & Technology

    2007-06-01

    07 Final _ 2/01/05 - 01/31/07 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER Uncertainty-based Design Methods for Flow- N00014-04-1-0007 Structure ...project is to develop advanced tools for efficient simulations of flow- structure interactions that account for random excitation and uncertain input...with emphasis on realistic three-dimensional nonlinear representatiol of the structures of interest. This capability will set the foundation for the

  18. Study of the Transition Flow Regime using Monte Carlo Methods

    NASA Technical Reports Server (NTRS)

    Hassan, H. A.

    1999-01-01

    This NASA Cooperative Agreement presents a study of the Transition Flow Regime Using Monte Carlo Methods. The topics included in this final report are: 1) New Direct Simulation Monte Carlo (DSMC) procedures; 2) The DS3W and DS2A Programs; 3) Papers presented; 4) Miscellaneous Applications and Program Modifications; 5) Solution of Transitional Wake Flows at Mach 10; and 6) Turbulence Modeling of Shock-Dominated Fows with a k-Enstrophy Formulation.

  19. Compressible flow calculations employing the Galerkin/least-squares method

    NASA Technical Reports Server (NTRS)

    Shakib, F.; Hughes, T. J. R.; Johan, Zdenek

    1989-01-01

    A multielement group, domain decomposition algorithm is presented for solving linear nonsymmetric systems arising in the finite-element analysis of compressible flows employing the Galerkin/least-squares method. The iterative strategy employed is based on the generalized minimum residual (GMRES) procedure originally proposed by Saad and Shultz. Two levels of preconditioning are investigated. Applications to problems of high-speed compressible flow illustrate the effectiveness of the scheme.

  20. Higher-Order Panel Method for Aerodynamic Flow Analysis

    NASA Technical Reports Server (NTRS)

    Erickson, L.; Carmichael, R. L.; Levin, A. D.; Magnus, A.; Epton, M.; Baruah, P.; Massena, B.; Bussoletti, J.; Sidwell, K.; Johnson, F.; hide

    1982-01-01

    PANAIR uses high-order panel method to predict inviscid subsonic or supersonic flows about arbitrary configuration. Panel method solves linear partial differential equation numerically by approximating configuration surface with panels on which unknown "singularity strengths" are defined. PANAIR includes advanced software technology as well as advanced aerodynamic technology.

  1. AN IMMERSED BOUNDARY METHOD FOR COMPLEX INCOMPRESSIBLE FLOWS

    EPA Science Inventory

    An immersed boundary method for time-dependant, three- dimensional, incompressible flows is presented in this paper. The incompressible Navier-Stokes equations are discretized using a low-diffusion flux splitting method for the inviscid fluxes and a second order central differenc...

  2. AN IMMERSED BOUNDARY METHOD FOR COMPLEX INCOMPRESSIBLE FLOWS

    EPA Science Inventory

    An immersed boundary method for time-dependant, three- dimensional, incompressible flows is presented in this paper. The incompressible Navier-Stokes equations are discretized using a low-diffusion flux splitting method for the inviscid fluxes and a second order central differenc...

  3. Higher-Order Panel Method for Aerodynamic Flow Analysis

    NASA Technical Reports Server (NTRS)

    Erickson, L.; Carmichael, R. L.; Levin, A. D.; Magnus, A.; Epton, M.; Baruah, P.; Massena, B.; Bussoletti, J.; Sidwell, K.; Johnson, F.; Zeppa, J.; Bates, G.; Clemens, D.; Derbyshire, T.; Purdon, D.; Chiang, D.; Rubbert, P.; Nelson, F.; Wai, J.; Tsurusaki, K.; Smith, N.; Snyder, J. R.; Sotomayer, W.; Dejongh, J.; Thomas, J. L.

    1982-01-01

    PANAIR uses high-order panel method to predict inviscid subsonic or supersonic flows about arbitrary configuration. Panel method solves linear partial differential equation numerically by approximating configuration surface with panels on which unknown "singularity strengths" are defined. PANAIR includes advanced software technology as well as advanced aerodynamic technology.

  4. An update on projection methods for transient incompressible viscous flow

    SciTech Connect

    Gresho, P.M.; Chan, S.T.

    1995-07-01

    Introduced in 1990 was the biharmonic equation (for the pressure) and the concomitant biharmonic miracle when transient incompressible viscous flow is solved approximately by a projection method. Herein is introduced the biharmonic catastrophe that sometimes occurs with these same projection methods.

  5. Method and apparatus for monitoring characteristics of a flow path having solid components flowing therethrough

    DOEpatents

    Hoskinson, Reed L [Rigby, ID; Svoboda, John M [Idaho Falls, ID; Bauer, William F [Idaho Falls, ID; Elias, Gracy [Idaho Falls, ID

    2008-05-06

    A method and apparatus is provided for monitoring a flow path having plurality of different solid components flowing therethrough. For example, in the harvesting of a plant material, many factors surrounding the threshing, separating or cleaning of the plant material and may lead to the inadvertent inclusion of the component being selectively harvested with residual plant materials being discharged or otherwise processed. In accordance with the present invention the detection of the selectively harvested component within residual materials may include the monitoring of a flow path of such residual materials by, for example, directing an excitation signal toward of flow path of material and then detecting a signal initiated by the presence of the selectively harvested component responsive to the excitation signal. The detected signal may be used to determine the presence or absence of a selected plant component within the flow path of residual materials.

  6. Comparison of Methods for Estimating Low Flow Characteristics of Streams

    USGS Publications Warehouse

    Tasker, Gary D.

    1987-01-01

    Four methods for estimating the 7-day, 10-year and 7-day, 20-year low flows for streams are compared by the bootstrap method. The bootstrap method is a Monte Carlo technique in which random samples are drawn from an unspecified sampling distribution defined from observed data. The nonparametric nature of the bootstrap makes it suitable for comparing methods based on a flow series for which the true distribution is unknown. Results show that the two methods based on hypothetical distribution (Log-Pearson III and Weibull) had lower mean square errors than did the G. E. P. Box-D. R. Cox transformation method or the Log-W. C. Boughton method which is based on a fit of plotting positions.

  7. Meshless lattice Boltzmann method for the simulation of fluid flows.

    PubMed

    Musavi, S Hossein; Ashrafizaadeh, Mahmud

    2015-02-01

    A meshless lattice Boltzmann numerical method is proposed. The collision and streaming operators of the lattice Boltzmann equation are separated, as in the usual lattice Boltzmann models. While the purely local collision equation remains the same, we rewrite the streaming equation as a pure advection equation and discretize the resulting partial differential equation using the Lax-Wendroff scheme in time and the meshless local Petrov-Galerkin scheme based on augmented radial basis functions in space. The meshless feature of the proposed method makes it a more powerful lattice Boltzmann solver, especially for cases in which using meshes introduces significant numerical errors into the solution, or when improving the mesh quality is a complex and time-consuming process. Three well-known benchmark fluid flow problems, namely the plane Couette flow, the circular Couette flow, and the impulsively started cylinder flow, are simulated for the validation of the proposed method. Excellent agreement with analytical solutions or with previous experimental and numerical results in the literature is observed in all the simulations. Although the computational resources required for the meshless method per node are higher compared to that of the standard lattice Boltzmann method, it is shown that for cases in which the total number of nodes is significantly reduced, the present method actually outperforms the standard lattice Boltzmann method.

  8. Interactive method for computation of viscous flow with recirculation

    NASA Technical Reports Server (NTRS)

    Brandeis, J.; Rom, J.

    1981-01-01

    An interactive method is proposed for the solution of two-dimensional, laminar flow fields with identifiable regions of recirculation, such as the shear-layer-driven cavity flow. The method treats the flow field as composed of two regions, with an appropriate mathematical model adopted for each region. The shear layer is computed by the compressible boundary layer equations, and the slowly recirculating flow by the incompressible Navier-Stokes equations. The flow field is solved iteratively by matching the local solutions in the two regions. For this purpose a new matching method utilizing an overlap between the two computational regions is developed, and shown to be most satisfactory. Matching of the two velocity components, as well as the change in velocity with respect to depth is amply accomplished using the present approach, and the stagnation points corresponding to separation and reattachment of the dividing streamline are computed as part of the interactive solution. The interactive method is applied to the test problem of a shear layer driven cavity. The computational results are used to show the validity and applicability of the present approach.

  9. A multi-domain method for subsonic viscous flows

    NASA Technical Reports Server (NTRS)

    Chan, Daniel C.; Sindir, Munir M.

    1992-01-01

    We have developed a Schwarz type domain decomposition method for a pressure base, two- and three-dimensional Navier-Stokes solver. This technique allows one to partition a flow path, which can be characterized by complex geometry and/or complicated flow physics, into smaller sub-domains according to the local geometric simplicity or estimated flow scales. We can, then, sweep the sub-domains in some order and solve the Navier-Stokes equations using as boundary conditions, along the domain interfaces, the Dirichlet conditions which are taken from the most recent update of the solution in the adjacent neighboring domains. With this technique, one can minimize the adverse effects caused by grid skewness and the stiffness problem caused by disparate flow scales. Here, we report the results of a few fundamental flow cases to demonstrate that a judicious use of the multi-domain method can offer a significant convergence acceleration over the traditional one-domain method. This method can be extended to exploit the architecture of a parallel computer to further improve the speed.

  10. Interactive method for computation of viscous flow with recirculation

    NASA Technical Reports Server (NTRS)

    Brandeis, J.; Rom, J.

    1981-01-01

    An interactive method is proposed for the solution of two-dimensional, laminar flow fields with identifiable regions of recirculation, such as the shear-layer-driven cavity flow. The method treats the flow field as composed of two regions, with an appropriate mathematical model adopted for each region. The shear layer is computed by the compressible boundary layer equations, and the slowly recirculating flow by the incompressible Navier-Stokes equations. The flow field is solved iteratively by matching the local solutions in the two regions. For this purpose a new matching method utilizing an overlap between the two computational regions is developed, and shown to be most satisfactory. Matching of the two velocity components, as well as the change in velocity with respect to depth is amply accomplished using the present approach, and the stagnation points corresponding to separation and reattachment of the dividing streamline are computed as part of the interactive solution. The interactive method is applied to the test problem of a shear layer driven cavity. The computational results are used to show the validity and applicability of the present approach.

  11. Gpu Implementation of Preconditioning Method for Low-Speed Flows

    NASA Astrophysics Data System (ADS)

    Zhang, Jiale; Chen, Hongquan

    2016-06-01

    An improved preconditioning method for low-Mach-number flows is implemented on a GPU platform. The improved preconditioning method employs the fluctuation of the fluid variables to weaken the influence of accuracy caused by the truncation error. The GPU parallel computing platform is implemented to accelerate the calculations. Both details concerning the improved preconditioning method and the GPU implementation technology are described in this paper. Then a set of typical low-speed flow cases are simulated for both validation and performance analysis of the resulting GPU solver. Numerical results show that dozens of times speedup relative to a serial CPU implementation can be achieved using a single GPU desktop platform, which demonstrates that the GPU desktop can serve as a cost-effective parallel computing platform to accelerate CFD simulations for low-Speed flows substantially.

  12. Electron-Beam Diagnostic Methods for Hypersonic Flow Diagnostics

    NASA Technical Reports Server (NTRS)

    1994-01-01

    The purpose of this work was the evaluation of the use of electron-bean fluorescence for flow measurements during hypersonic flight. Both analytical and numerical models were developed in this investigation to evaluate quantitatively flow field imaging concepts based upon the electron beam fluorescence technique for use in flight research and wind tunnel applications. Specific models were developed for: (1) fluorescence excitation/emission for nitrogen, (2) rotational fluorescence spectrum for nitrogen, (3) single and multiple scattering of electrons in a variable density medium, (4) spatial and spectral distribution of fluorescence, (5) measurement of rotational temperature and density, (6) optical filter design for fluorescence imaging, and (7) temperature accuracy and signal acquisition time requirements. Application of these models to a typical hypersonic wind tunnel flow is presented. In particular, the capability of simulating the fluorescence resulting from electron impact ionization in a variable density nitrogen or air flow provides the capability to evaluate the design of imaging instruments for flow field mapping. The result of this analysis is a recommendation that quantitative measurements of hypersonic flow fields using electron-bean fluorescence is a tractable method with electron beam energies of 100 keV. With lower electron energies, electron scattering increases with significant beam divergence which makes quantitative imaging difficult. The potential application of the analytical and numerical models developed in this work is in the design of a flow field imaging instrument for use in hypersonic wind tunnels or onboard a flight research vehicle.

  13. Mixed exhaust flow supersonic jet engine and method

    SciTech Connect

    Klees, G.W.

    1993-06-08

    A method of operating a supersonic jet engine installation is described comprising (a) providing an engine having a variable area air inlet means and an outlet to discharge engine exhaust; (b) providing a secondary air passageway means; (c) receiving ambient air in the air inlet means and providing the ambient air as primary air to the engine inlet and secondary air to the secondary air passageway means; (d) providing a mixing section having an inlet portion and an exit portion, utilizing the mixing section in directing the exhaust from the engine to primary convergent/divergent exit passageway segments, where the exhaust is discharged at supersonic velocity as primary flow components, and directing secondary air flow from the secondary air passageway means to secondary exit passageway segments which are interspersed with the primary segments and from which the secondary air is discharged at subsonic velocity as secondary flow components; and (e) providing an exhaust section to receive the primary and secondary flow components in a mixing region and causing the primary and secondary flow components to mix to create a supersonic mixed flow, the exhaust section having a variable area final nozzle through which the mixed flow is discharged.

  14. Simulation of turbulent flows using nodal integral method

    NASA Astrophysics Data System (ADS)

    Singh, Suneet

    Nodal methods are the backbone of the production codes for neutron-diffusion and transport equations. Despite their high accuracy, use of these methods for simulation of fluid flow is relatively new. Recently, a modified nodal integral method (MNIM) has been developed for simulation of laminar flows. In view of its high accuracy and efficiency, extension of this method for the simulation of turbulent flows is a logical step forward. In this dissertation, MNIM is extended in two ways to simulate incompressible turbulent flows---a new MNIM is developed for the 2D k-epsilon equations; and 3D, parallel MNIM is developed for direct numerical simulations. Both developments are validated, and test problems are solved. In this dissertation, a new nodal numerical scheme is developed to solve the k-epsilon equations to simulate turbulent flows. The MNIM developed earlier for laminar flow equations is modified to incorporate eddy viscosity approximation and coupled with the above mentioned schemes for the k and epsilon equations, to complete the implementation of the numerical scheme for the k-epsilon model. The scheme developed is validated by comparing the results obtained by the developed method with the results available in the literature obtained using direct numerical simulations (DNS). The results of current simulations match reasonably well with the DNS results. The discrepancies in the results are mainly due to the limitations of the k-epsilon model rather than the deficiency in the developed MNIM. A parallel version of the MNIM is needed to enhance its capability, in order to carry out DNS of the turbulent flows. The parallelization of the scheme, however, presents some unique challenges as dependencies of the discrete variables are different from those that exist in other schemes (for example in finite volume based schemes). Hence, a parallel MNIM (PMNIM) is developed and implemented into a computer code with communication strategies based on the above mentioned

  15. Combustor air flow control method for fuel cell apparatus

    DOEpatents

    Clingerman, Bruce J.; Mowery, Kenneth D.; Ripley, Eugene V.

    2001-01-01

    A method for controlling the heat output of a combustor in a fuel cell apparatus to a fuel processor where the combustor has dual air inlet streams including atmospheric air and fuel cell cathode effluent containing oxygen depleted air. In all operating modes, an enthalpy balance is provided by regulating the quantity of the air flow stream to the combustor to support fuel cell processor heat requirements. A control provides a quick fast forward change in an air valve orifice cross section in response to a calculated predetermined air flow, the molar constituents of the air stream to the combustor, the pressure drop across the air valve, and a look up table of the orifice cross sectional area and valve steps. A feedback loop fine tunes any error between the measured air flow to the combustor and the predetermined air flow.

  16. Method for selectively controlling flow across slotted liners

    SciTech Connect

    Peavy, M.A.; Dees, J.M.

    1993-08-31

    A process is described for decreasing flow rate across the radial boundary of a selected interval in a well bore containing a slotted liner comprising: placing an explosive and an internally catalyzed resin solution inside an elongated container; locating the elongated container opposite the selected interval in the well bore where flow rate through the slotted liner is to be decreased; firing the explosive; and allowing the resin to cure on the slotted liner before initiating flow through the well. A method is described for decreasing production of unwanted fluids from a horizontal well containing a slotted liner comprising: placing an explosive and an internally catalyzed resin inside an elongated container; placing the elongated container opposite an interval in the horizontal well where unwanted fluid is entering the well bore through the slotted liner; firing the explosive; and permitting the resin to cure on the slotted liner before initiating flow in the well.

  17. Continuous-flow free acid monitoring method and system

    DOEpatents

    Strain, J.E.; Ross, H.H.

    1980-01-11

    A free acid monitoring method and apparatus is provided for continuously measuring the excess acid present in a process stream. The disclosed monitoring system and method is based on the relationship of the partial pressure ratio of water and acid in equilibrium with an acid solution at constant temperature. A portion of the process stream is pumped into and flows through the monitor under the influence of gravity and back to the process stream. A continuous flowing sample is vaporized at a constant temperature and the vapor is subsequently condensed. Conductivity measurements of the condensate produces a nonlinear response function from which the free acid molarity of the sample process stream is determined.

  18. Continuous-flow free acid monitoring method and system

    DOEpatents

    Strain, James E.; Ross, Harley H.

    1981-01-01

    A free acid monitoring method and apparatus is provided for continuously measuring the excess acid present in a process stream. The disclosed monitoring system and method is based on the relationship of the partial pressure ratio of water and acid in equilibrium with an acid solution at constant temperature. A portion of the process stream is pumped into and flows through the monitor under the influence of gravity and back to the process stream. A continuous flowing sample is vaporized at a constant temperature and the vapor is subsequently condensed. Conductivity measurements of the condensate produces a nonlinear response function from which the free acid molarity of the sample process stream is determined.

  19. Systems and methods for rebalancing redox flow battery electrolytes

    DOEpatents

    Pham, Ai Quoc; Chang, On Kok

    2015-03-17

    Various methods of rebalancing electrolytes in a redox flow battery system include various systems using a catalyzed hydrogen rebalance cell configured to minimize the risk of dissolved catalyst negatively affecting flow battery performance. Some systems described herein reduce the chance of catalyst contamination of RFB electrolytes by employing a mediator solution to eliminate direct contact between the catalyzed membrane and the RFB electrolyte. Other methods use a rebalance cell chemistry that maintains the catalyzed electrode at a potential low enough to prevent the catalyst from dissolving.

  20. Improved numerical methods for turbulent viscous recirculating flows

    NASA Technical Reports Server (NTRS)

    Turan, A.; Vandoormaal, J. P.

    1988-01-01

    The performance of discrete methods for the prediction of fluid flows can be enhanced by improving the convergence rate of solvers and by increasing the accuracy of the discrete representation of the equations of motion. This report evaluates the gains in solver performance that are available when various acceleration methods are applied. Various discretizations are also examined and two are recommended because of their accuracy and robustness. Insertion of the improved discretization and solver accelerator into a TEACH mode, that has been widely applied to combustor flows, illustrates the substantial gains to be achieved.

  1. Processing and analysis methods for transonic cavity flow

    NASA Astrophysics Data System (ADS)

    Loupy, G. J. M.; Barakos, G. N.

    2017-07-01

    This paper focuses on the localisation of noise sources in transonic cavity flows. Beamforming is used to estimate the pressure fluctuations inside a resonant transonic cavity, showing the localisation of the main sources of noise using an acoustic array and also combining it with a mean flow-field. The influence of the microphone array position, density, and shape is investigated. The presented method models the noise propagation with simple assumptions that are easily applicable to wind tunnel testing and may help localise the noise sources from complex geometries without intrusive methods.

  2. An eddy viscosity calculation method for a turbulent duct flow

    NASA Technical Reports Server (NTRS)

    Antonia, R. A.; Bisset, D. K.; Kim, J.

    1991-01-01

    The mean velocity profile across a fully developed turbulent duct flow is obtained from an eddy viscosity relation combined with an empirical outer region wake function. Results are in good agreement with experiments and with direct numerical simulations in the same flow at two Reynolds numbers. In particular, the near-wall trend of the Reynolds shear stress and its variation with Reynolds number are similar to those of the simulations. The eddy viscosity method is more accurate than previous mixing length or implicit function methods.

  3. Fully consistent CFD methods for incompressible flow computations

    NASA Astrophysics Data System (ADS)

    Kolmogorov, D. K.; Shen, W. Z.; Sørensen, N. N.; Sørensen, J. N.

    2014-06-01

    Nowadays collocated grid based CFD methods are one of the most efficient tools for computations of the flows past wind turbines. To ensure the robustness of the methods they require special attention to the well-known problem of pressure-velocity coupling. Many commercial codes to ensure the pressure-velocity coupling on collocated grids use the so-called momentum interpolation method of Rhie and Chow [1]. As known, the method and some of its widely spread modifications result in solutions, which are dependent of time step at convergence. In this paper the magnitude of the dependence is shown to contribute about 0.5% into the total error in a typical turbulent flow computation. Nevertheless if coarse grids are used, the standard interpolation methods result in much higher non-consistent behavior. To overcome the problem, a recently developed interpolation method, which is independent of time step, is used. It is shown that in comparison to other time step independent method, the method may enhance the convergence rate of the SIMPLEC algorithm up to 25 %. The method is verified using turbulent flow computations around a NACA 64618 airfoil and the roll-up of a shear layer, which may appear in wind turbine wake.

  4. Adaptive computational methods for SSME internal flow analysis

    NASA Technical Reports Server (NTRS)

    Oden, J. T.

    1986-01-01

    Adaptive finite element methods for the analysis of classes of problems in compressible and incompressible flow of interest in SSME (space shuttle main engine) analysis and design are described. The general objective of the adaptive methods is to improve and to quantify the quality of numerical solutions to the governing partial differential equations of fluid dynamics in two-dimensional cases. There are several different families of adaptive schemes that can be used to improve the quality of solutions in complex flow simulations. Among these are: (1) r-methods (node-redistribution or moving mesh methods) in which a fixed number of nodal points is allowed to migrate to points in the mesh where high error is detected; (2) h-methods, in which the mesh size h is automatically refined to reduce local error; and (3) p-methods, in which the local degree p of the finite element approximation is increased to reduce local error. Two of the three basic techniques have been studied in this project: an r-method for steady Euler equations in two dimensions and a p-method for transient, laminar, viscous incompressible flow. Numerical results are presented. A brief introduction to residual methods of a-posterior error estimation is also given and some pertinent conclusions of the study are listed.

  5. Finite element methods of studying mechanical factors in blood flow.

    PubMed

    Davids, N

    1981-01-01

    This paper reviews some biomechanical analyses of blood flow in large arteries based on a general computer modeling using the finite element method. We study the following question: What is the role played by the interrelated factors of mechanical stress, flow irregularities, and diffusion through the endothelium on the etiology of atherosclerosis or the aggravation of vascular injury. It presents the computational features of the method and stresses the physiological significance of the results, such as the effect of geometric complexities, material nonlinearities, and non-Newtonian rheology of the blood. The specific mechanical and fluid dynamic factors analyzed are wall shear stress, flow profiles, and pressure variations. After simulating tubes of circular cross section, we apply the analysis to a number of physiological situations of significance, including blood flow in the entrance region, at bifurcations, in the annular region between an inserted catheter of varying diameter and the vessel. A model study of pulsatile flow in a 60 degree bifurcated channel of velocity profiles provided corroborative measurements of these processes with special emphasis on reversed or distributed flow conditions. The corresponding analysis was extended to the situation in which flow separates and reverses in the neighborhood of stagnation points. This required developing the nonlinear expression for the convective velocity change in the medium. A computer algorithm was developed to handle simultaneous effects of pressure and viscous forces on velocity change across the element and applied to the canine prebranch arterial segment. For mean physiological flow conditions, low shear stresses (0-10 dynes/cm2) are predicted near the wall in the diverging plane, higher values (50 dynes/cm2) along the converging sides of the wall. Backflow is predicted along the outer wall, pressure recovery prior to and into the branches, and a peak shear at the divider lip.

  6. Calibration of base flow separation methods with streamflow conductivity.

    PubMed

    Stewart, Mark; Cimino, Joseph; Ross, Mark

    2007-01-01

    The conductivity mass-balance (CMB) method can be used to calibrate analytical base flow separation methods. The principal CMB assumptions are base flow conductivity is equal to streamflow conductivity at lowest flows, runoff conductivity is equal to streamflow conductivity at highest flows, and base flow and runoff conductivities are assumed to be constants over the period of record. To test the CMB assumptions, fluid conductivities of ground water, surface runoff, and streamflow were measured during wet and dry conditions in a 12-km(2) stream basin. Ground water conductivities at wells varied an average of 6% from dry to wet conditions, while stream conductivities varied 58%. Shallow ground water conductivity varied significantly with distance from the stream, with lowest conductivities of 87 microS/cm near the divide, a maximum of 520 microS/cm 59 m from the stream, and 215 microS/cm 22 m from the stream. Runoff conductivities measured in three rain events remained nearly constant, with lower conductivities of 35 microS/cm near the divide and 50 microS/cm near the stream. The CMB method was applied to the records from 10 USGS stream-gauging stations in Texas, Kentucky, Georgia, and Florida to calibrate the USGS base flow separation technique, HYSEP, by varying the time parameter 2N*. There is a statistically significant relationship between basin areas and calibrated values of 2N*, expressed as N = 0.46A(0.44), with N in days and A in km(2). The widely accepted relationship N = 0.83A(0.2) is not valid for these basins. Other analytic methods can also be calibrated with the CMB method.

  7. A sharp interface immersed boundary method for compressible viscous flows

    NASA Astrophysics Data System (ADS)

    Ghias, R.; Mittal, R.; Dong, H.

    2007-07-01

    An immersed boundary method for computing viscous, subsonic compressible flows with complex shaped stationary immersed boundaries is presented. The method employs a ghost-cell technique for imposing the boundary conditions on the immersed boundaries. The current approach leads to a sharp representation of the immersed boundaries, a property that is especially useful for flow simulations at high Reynolds numbers. Another unique feature of the method is that it can be applied on Cartesian as well as generalized body non-conformal curvilinear meshes. A mixed second-order central difference-QUICK scheme is used which allows a high degree of control over the numerical damping. A bilinear interpolation scheme used in conjunction with the ghost-cell approach results in second-order global as well as local spatial accuracy. The solver is parallelized for distributed memory platforms using domain decomposition and message passing interface (MPI) and salient features of the parallel algorithm are presented. The accuracy, fidelity and efficiency of the solver are examined by simulating flow past circular cylinders and airfoils and comparing against experimental data and other established results. Finally, we present results from a simulation of wing-tip flow at a relatively high Reynolds number in order to demonstrate the ability of the solver to model complex, non-canonical three-dimensional flows.

  8. A simulated dye method for flow visualization with a computational model for blood flow.

    PubMed

    Kim, T; Cheer, A Y; Dwyer, H A

    2004-08-01

    A numerical dye method for the visualization of unsteady three-dimensional flow calculations is introduced by coupling the unsteady convection-diffusion equation to the Navier-Stokes equation for mass and momentum. This system of equations is descretized using a finite volume projection-like algorithm with generalized coordinates and overset grids. A powerful pressure prediction method is used to accelerate the convergence of the Pressure Poisson equation. To demonstrate the visualization technique, blood flow through the aortic arch region and the three main arterial branches is computed using various Womersley numbers. In this technique, parcels of fluid are followed in time as a function of the cardiac cycle without having to track individual particles, which in turn aids us to better understand some important aspects of the three-dimensionality of the developing unsteady flow. Using this numerical dye method we analyze the strength of the cross flow during the cardiac cycle, the relationship between the penetration of blood into the aortic branches from its relative position in the ascending aortic region and the effects of the Womersley parameter. This technique can be very useful in the design and development of stents where the topology of the device would require understanding where the blood emanating from the heart ends up at the end of the cardiac cycle. Moreover, this method could be useful in investigating the influence of flow and geometry on the local introduction of medication.

  9. Development of flow/steric field-flow fractionation as a routine process control method

    SciTech Connect

    Barman, B.N.

    1988-08-30

    Researchers studied the feasibility of using the Flow/Steric Field-Flow Fractionation (Flow/StFFF) method for the characterization of particulate materials with diameters in the 1-100 micrometers range. Studies on the optimization of the method for the separation and characterization of different size particulate samples, as well as on the role of the crossflow field and channel flowrate on the separation and resolution, were performed with a number of spherical polystyrene divinylbenzene latex standards and included in the report. Applicability of the method as a fast and reliable practical tool for industrial process control, particularly for grinding operations, was examined by analyzing a number of samples obtained by grinding. Examples of materials considered include coal, limestone and glass.

  10. A flow cytometric method for platelet counting in platelet concentrates.

    PubMed

    van der Meer, Pieter F; Karssing-van Leeuwen, Willy; Kurtz, Jim; Spengler, Hans-Peter; Blair, AbbeJane; Devine, Dana; Harrison, Paul; Lambrecht, Bernd; VandenBroeke, Tania; de Wildt, Janny; de Korte, Dirk

    2012-01-01

    The platelets (PLTs) in PLT concentrates are counted with hematology analyzers, but varying results among different hematology analyzers are observed, making comparisons very difficult. Due to the absence of red blood cells in PLT concentrates, the International Council for Standardization in Hematology (ICSH) reference method was modified to be used for PLT concentrates and validated in an international comparative study. Five PLT samples were shipped to eight participating centers of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative and counted on the same day. PLTs were stained with fluorescein isothiocyanate-labeled anti-CD41a in tubes (TruCount, BD Biosciences), measured on a flow cytometer, and analyzed with a uniform template. These samples were also counted on 15 hematology analyzers. The ICSH method and newly developed BEST method yielded PLT counting results with less than 1% difference (not significant). The intercenter coefficient of variation (CV) of the BEST method was on average 6.3% versus 7.6% on average for hematology analyzers. The CV of individual hematology analyzers was on average 0.9%, which was considerably lower than for the flow cytometers with a mean of 3.7%. The BEST flow cytometric method has a smaller intercenter CV and a smaller center-to-center deviation from the group mean compared to hematology analyzers. Conversely, individual hematology analyzers are more precise than the flow cytometric method. Thus, the flow cytometric method provides a calibration tool to allow comparisons between centers, but there is no need to replace routine counting with hematology analyzers. © 2011 American Association of Blood Banks.

  11. Methods for estimating low-flow statistics for Massachusetts streams

    USGS Publications Warehouse

    Ries, Kernell G.; Friesz, Paul J.

    2000-01-01

    Methods and computer software are described in this report for determining flow duration, low-flow frequency statistics, and August median flows. These low-flow statistics can be estimated for unregulated streams in Massachusetts using different methods depending on whether the location of interest is at a streamgaging station, a low-flow partial-record station, or an ungaged site where no data are available. Low-flow statistics for streamgaging stations can be estimated using standard U.S. Geological Survey methods described in the report. The MOVE.1 mathematical method and a graphical correlation method can be used to estimate low-flow statistics for low-flow partial-record stations. The MOVE.1 method is recommended when the relation between measured flows at a partial-record station and daily mean flows at a nearby, hydrologically similar streamgaging station is linear, and the graphical method is recommended when the relation is curved. Equations are presented for computing the variance and equivalent years of record for estimates of low-flow statistics for low-flow partial-record stations when either a single or multiple index stations are used to determine the estimates. The drainage-area ratio method or regression equations can be used to estimate low-flow statistics for ungaged sites where no data are available. The drainage-area ratio method is generally as accurate as or more accurate than regression estimates when the drainage-area ratio for an ungaged site is between 0.3 and 1.5 times the drainage area of the index data-collection site. Regression equations were developed to estimate the natural, long-term 99-, 98-, 95-, 90-, 85-, 80-, 75-, 70-, 60-, and 50-percent duration flows; the 7-day, 2-year and the 7-day, 10-year low flows; and the August median flow for ungaged sites in Massachusetts. Streamflow statistics and basin characteristics for 87 to 133 streamgaging stations and low-flow partial-record stations were used to develop the equations. The

  12. A reconstruction method of intra-ventricular blood flow using color flow ultrasound: a simulation study

    NASA Astrophysics Data System (ADS)

    Jang, Jaeseong; Ahn, Chi Young; Jeon, Kiwan; Choi, Jung-il; Lee, Changhoon; Seo, Jin Keun

    2015-03-01

    A reconstruction method is proposed here to quantify the distribution of blood flow velocity fields inside the left ventricle from color Doppler echocardiography measurement. From 3D incompressible Navier- Stokes equation, a 2D incompressible Navier-Stokes equation with a mass source term is derived to utilize the measurable color flow ultrasound data in a plane along with the moving boundary condition. The proposed model reflects out-of-plane blood flows on the imaging plane through the mass source term. For demonstrating a feasibility of the proposed method, we have performed numerical simulations of the forward problem and numerical analysis of the reconstruction method. First, we construct a 3D moving LV region having a specific stroke volume. To obtain synthetic intra-ventricular flows, we performed a numerical simulation of the forward problem of Navier-Stokes equation inside the 3D moving LV, computed 3D intra-ventricular velocity fields as a solution of the forward problem, projected the 3D velocity fields on the imaging plane and took the inner product of the 2D velocity fields on the imaging plane and scanline directional velocity fields for synthetic scanline directional projected velocity at each position. The proposed method utilized the 2D synthetic projected velocity data for reconstructing LV blood flow. By computing the difference between synthetic flow and reconstructed flow fields, we obtained the averaged point-wise errors of 0.06 m/s and 0.02 m/s for u- and v-components, respectively.

  13. Fourier-Legendre spectral methods for incompressible channel flow

    NASA Technical Reports Server (NTRS)

    Zang, T. A.; Hussaini, M. Y.

    1984-01-01

    An iterative collocation technique is described for modeling implicit viscosity in three-dimensional incompressible wall bounded shear flow. The viscosity can vary temporally and in the vertical direction. Channel flow is modeled with a Fourier-Legendre approximation and the mean streamwise advection is treated implicitly. Explicit terms are handled with an Adams-Bashforth method to increase the allowable time-step for calculation of the implicit terms. The algorithm is applied to low amplitude unstable waves in a plane Poiseuille flow at an Re of 7500. Comparisons are made between results using the Legendre method and with Chebyshev polynomials. Comparable accuracy is obtained for the perturbation kinetic energy predicted using both discretizations.

  14. Time-derivative preconditioning method for multicomponent flow

    NASA Astrophysics Data System (ADS)

    Housman, Jeffrey Allen

    A time-derivative preconditioned system of equations suitable for the numerical simulation of single component and multicomponent inviscid flows at all speeds is formulated. The system is shown to be hyperbolic in time and remain well-posed at low Mach numbers, allowing an efficient time marching solution strategy to be utilized from transonic to incompressible flow speeds. For multicomponent flow at low speed, a preconditioned nonconservative discretization scheme is described which preserves pressure and velocity equilibrium across fluid interfaces, handles sharp liquid/gas interfaces with large density ratios, while remaining well-conditioned for time marching methods. The method is then extended to transonic and supersonic flows using a hybrid conservative/nonconservative formulation which retains the pressure/velocity equilibrium property and converges to the correct weak solution when shocks are present. In order to apply the proposed model to complex flow applications, the overset grid methodology is used where the equations are transformed to a nonorthogonal curvilinear coordinate system and discretized on structured body-fitted curvilinear grids. The multicomponent model and its extension to homogeneous multiphase mixtures is discussed and the hyperbolicity of the governing equations is demonstrated. Low Mach number perturbation analysis is then performed on the system of equations and a local time-derivative preconditioning matrix is derived allowing time marching numerical methods to remain efficient at low speeds. Next, a particular time marching numerical method is presented along with three discretization schemes for the convective terms. These include a conservative preconditioned Roe type method, a nonconservative preconditioned Split Coefficient Matrix (SCM) method, and hybrid formulation which combines the conservative and nonconservative schemes using a simple switching function. A characteristic boundary treatment which includes time

  15. Method and apparatus for measuring coupled flow, transport, and reaction processes under liquid unsaturated flow conditions

    DOEpatents

    McGrail, Bernard P.; Martin, Paul F.; Lindenmeier, Clark W.

    1999-01-01

    The present invention is a method and apparatus for measuring coupled flow, transport and reaction processes under liquid unsaturated flow conditions. The method and apparatus of the present invention permit distinguishing individual precipitation events and their effect on dissolution behavior isolated to the specific event. The present invention is especially useful for dynamically measuring hydraulic parameters when a chemical reaction occurs between a particulate material and either liquid or gas (e.g. air) or both, causing precipitation that changes the pore structure of the test material.

  16. The numerical methods for the fluid flow of UCMCWS

    SciTech Connect

    Zhang Wenfu; Li Hui; Zhu Shuquan; Wang Zuna

    1997-12-31

    As an alternative for diesel oil for internal combustion engines, the fluid flow state of Ultra Clean Micronized Coal-Water Slurry (UCMCWS) in mini pipe and nozzle of a diesel engine must be known. In the laboratory three kinds of UCMCWS have been made with coal containing less than 0.8% ash, viscosity less than 600 mPa.s and concentration between 50% and 56%. Because the UCMCWS is a non-Newtonian fluid, there are no analytical resolution for pipe flow, especially in inlet and outlet sections. In this case using the numerical methods to research the flow state of UCMCWS is a useful method. Using the method of finite element, the flow state of UCMCWS in inlet and outlet sections (similar to a nozzle) have been studied. The distribution of velocity at different pressures of UCMCWS in outlet and inlet sections have been obtained. The result of the numerical methods is the efficient base for the pipe and nozzle design.

  17. Numerical methods and calculations for droplet flow, heating and ignition

    NASA Technical Reports Server (NTRS)

    Dwyer, H. A.; Sanders, B. R.; Dandy, D.

    1982-01-01

    A numerical method was devised and employed to solve a variety of problems related to liquid droplet combustion. The basic transport equations of mass, momentum and energy were formulated in terms of generalized nonorthogonal coordinates, which allows for adaptive griding and arbitrary particle shape. Example problems are solved for internal droplet heating, droplet ignition and high Reynolds number flow over a droplet.

  18. Validation of an Impedance Education Method in Flow

    NASA Technical Reports Server (NTRS)

    Watson, Willie R.; Jones, Michael G.; Parrott, Tony L.

    2004-01-01

    This paper reports results of a research effort to validate a method for educing the normal incidence impedance of a locally reacting liner, located in a grazing incidence, nonprogressive acoustic wave environment with flow. The results presented in this paper test the ability of the method to reproduce the measured normal incidence impedance of a solid steel plate and two soft test liners in a uniform flow. The test liners are known to be locally react- ing and exhibit no measurable amplitude-dependent impedance nonlinearities or flow effects. Baseline impedance spectra for these liners were therefore established from measurements in a conventional normal incidence impedance tube. A key feature of the method is the expansion of the unknown impedance function as a piecewise continuous polynomial with undetermined coefficients. Stewart's adaptation of the Davidon-Fletcher-Powell optimization algorithm is used to educe the normal incidence impedance at each Mach number by optimizing an objective function. The method is shown to reproduce the measured normal incidence impedance spectrum for each of the test liners, thus validating its usefulness for determining the normal incidence impedance of test liners for a broad range of source frequencies and flow Mach numbers. Nomenclature

  19. Lattice Boltzmann method for linear oscillatory noncontinuum flows

    NASA Astrophysics Data System (ADS)

    Shi, Yong; Yap, Ying Wan; Sader, John E.

    2014-03-01

    Oscillatory gas flows are commonly generated by micro- and nanoelectromechanical systems. Due to their small size and high operating frequencies, these devices often produce noncontinuum gas flows. Theoretical analysis of such flows requires solution of the unsteady Boltzmann equation, which can present a formidable challenge. In this article, we explore the applicability of the lattice Boltzmann (LB) method to such linear oscillatory noncontinuum flows; this method is derived from the linearized Boltzmann Bhatnagar-Gross-Krook (BGK) equation. We formulate four linearized LB models in the frequency domain, based on Gaussian-Hermite quadratures of different algebraic precision (AP). The performance of each model is assessed by comparison to high-accuracy numerical solutions to the linearized Boltzmann-BGK equation for oscillatory Couette flow. The numerical results demonstrate that high even-order LB models provide superior performance over the greatest noncontinuum range. Our results also highlight intrinsic deficiencies in the current LB framework, which is incapable of capturing noncontinuum behavior at high oscillation frequencies, regardless of quadrature AP and the Knudsen number.

  20. Multicomponent-flow analyses by multimode method of characteristics

    USGS Publications Warehouse

    Lai, Chintu

    1994-01-01

    For unsteady open-channel flows having N interacting unknown variables, a system of N mutually independent, partial differential equations can be used to describe the flow-field. The system generally belongs to marching-type problems and permits transformation into characteristic equations that are associated with N distinct characteristics directions. Because characteristics can be considered 'wave' or 'disturbance' propagation, a fluvial system so described can be viewed as adequately definable using these N component waves. A numerical algorithm to solve the N families of characteristics can then be introduced for formulation of an N-component flow-simulation model. The multimode method of characteristics (MMOC), a new numerical scheme that has a combined capacity of several specified-time-interval (STI) schemes of the method of characteristics, makes numerical modeling of such N-component riverine flows feasible and attainable. Merging different STI schemes yields different kinds of MMOC schemes, for which two kinds are displayed herein. With the MMOC, each characteristics is dynamically treated by an appropriate numerical mode, which should lead to an effective and suitable global simulation, covering various types of unsteady flow. The scheme is always linearly stable and its numerical accuracy can be systematically analyzed. By increasing the N value, one can develop a progressively sophisticated model that addresses increasingly complex river-mechanics problems.

  1. Lattice Boltzmann method for linear oscillatory noncontinuum flows.

    PubMed

    Shi, Yong; Yap, Ying Wan; Sader, John E

    2014-03-01

    Oscillatory gas flows are commonly generated by micro- and nanoelectromechanical systems. Due to their small size and high operating frequencies, these devices often produce noncontinuum gas flows. Theoretical analysis of such flows requires solution of the unsteady Boltzmann equation, which can present a formidable challenge. In this article, we explore the applicability of the lattice Boltzmann (LB) method to such linear oscillatory noncontinuum flows; this method is derived from the linearized Boltzmann Bhatnagar-Gross-Krook (BGK) equation. We formulate four linearized LB models in the frequency domain, based on Gaussian-Hermite quadratures of different algebraic precision (AP). The performance of each model is assessed by comparison to high-accuracy numerical solutions to the linearized Boltzmann-BGK equation for oscillatory Couette flow. The numerical results demonstrate that high even-order LB models provide superior performance over the greatest noncontinuum range. Our results also highlight intrinsic deficiencies in the current LB framework, which is incapable of capturing noncontinuum behavior at high oscillation frequencies, regardless of quadrature AP and the Knudsen number.

  2. Time spectral method for rotorcraft flow with vorticity confinement

    NASA Astrophysics Data System (ADS)

    Butsuntorn, Nawee

    2008-10-01

    This thesis shows that simulation of helicopter flows can adhere to engineering accuracy without the need of massive computing resources or long turnaround time by choosing an alternative framework for rotorcraft simulation. The method works in both hovering and forward flight regimes. The new method has shown to be more computationally efficient and sufficiently accurate. By utilizing the periodic nature of the rotorcraft flow field, the Fourier based Time Spectral method lends itself to the problem and significantly increases the rate of convergence compared to traditional implicit time integration schemes such as the second order backward difference formula (BDF). A Vorticity Confinement method has been explored and has been shown to work well in subsonic and transonic simulations. Vortical structure can be maintained after long distances without resorting to the traditional mesh refinement technique.

  3. New Methods for Sensitivity Analysis in Chaotic, Turbulent Fluid Flows

    NASA Astrophysics Data System (ADS)

    Blonigan, Patrick; Wang, Qiqi

    2012-11-01

    Computational methods for sensitivity analysis are invaluable tools for fluid mechanics research and engineering design. These methods are used in many applications, including aerodynamic shape optimization and adaptive grid refinement. However, traditional sensitivity analysis methods break down when applied to long-time averaged quantities in chaotic fluid flowfields, such as those obtained using high-fidelity turbulence simulations. Also, a number of dynamical properties of chaotic fluid flows, most notably the ``Butterfly Effect,'' make the formulation of new sensitivity analysis methods difficult. This talk will outline two chaotic sensitivity analysis methods. The first method, the Fokker-Planck adjoint method, forms a probability density function on the strange attractor associated with the system and uses its adjoint to find gradients. The second method, the Least Squares Sensitivity method, finds some ``shadow trajectory'' in phase space for which perturbations do not grow exponentially. This method is formulated as a quadratic programing problem with linear constraints. This talk is concluded with demonstrations of these new methods on some example problems, including the Lorenz attractor and flow around an airfoil at a high angle of attack.

  4. A Multi-domain Spectral Method for Supersonic Reactive Flows

    NASA Technical Reports Server (NTRS)

    Don, Wai-Sun; Gottlieb, David; Jung, Jae-Hun; Bushnell, Dennis M. (Technical Monitor)

    2002-01-01

    This paper has a dual purpose: it presents a multidomain Chebyshev method for the solution of the two-dimensional reactive compressible Navier-Stokes equations, and it reports the results of the application of this code to the numerical simulations of high Mach number reactive flows in recessed cavity. The computational method utilizes newly derived interface boundary conditions as well as an adaptive filtering technique to stabilize the computations. The results of the simulations are relevant to recessed cavity flameholders.

  5. Modeling electrokinetic flow by Lagrangian particle-based method

    NASA Astrophysics Data System (ADS)

    Pan, Wenxiao; Kim, Kyungjoo; Perego, Mauro; Tartakovsky, Alexandre; Parks, Mike

    2015-11-01

    This work focuses on mathematical models and numerical schemes based on Lagrangian particle-based method that can effectively capture mesoscale multiphysics (hydrodynamics, electrostatics, and advection-diffusion) associated in applications of micro-/nano-transport and technology. The order of accuracy is significantly improved for particle-based method with the presented implicit consistent numerical scheme. Specifically, we show simulation results on electrokinetic flows and microfluidic mixing processes in micro-/nano-channel and through semi-permeable porous structures.

  6. Methods of Visually Determining the Air Flow Around Airplanes

    NASA Technical Reports Server (NTRS)

    Gough, Melvin N; Johnson, Ernest

    1932-01-01

    This report describes methods used by the National Advisory Committee for Aeronautics to study visually the air flow around airplanes. The use of streamers, oil and exhaust gas streaks, lampblack and kerosene, powdered materials, and kerosene smoke is briefly described. The generation and distribution of smoke from candles and from titanium tetrachloride are described in greater detail because they appear most advantageous for general application. Examples are included showing results of the various methods.

  7. Adaptive Spectral Estimation Methods in Color Flow Imaging.

    PubMed

    Karabiyik, Yucel; Ekroll, Ingvild Kinn; Eik-Nes, Sturla H; Avdal, Jorgen; Lovstakken, Lasse

    2016-11-01

    Clutter rejection for color flow imaging (CFI) remains a challenge due to either a limited amount of temporal samples available or nonstationary tissue clutter. This is particularly the case for interleaved CFI and B-mode acquisitions. Low velocity blood signal is attenuated along with the clutter due to the long transition band of the available clutter filters, causing regions of biased mean velocity estimates or signal dropouts. This paper investigates how adaptive spectral estimation methods, Capon and blood iterative adaptive approach (BIAA), can be used to estimate the mean velocity in CFI without prior clutter filtering. The approach is based on confining the clutter signal in a narrow spectral region around the zero Doppler frequency while keeping the spectral side lobes below the blood signal level, allowing for the clutter signal to be removed by thresholding in the frequency domain. The proposed methods are evaluated using computer simulations, flow phantom experiments, and in vivo recordings from the common carotid and jugular vein of healthy volunteers. Capon and BIAA methods could estimate low blood velocities, which are normally attenuated by polynomial regression filters, and may potentially give better estimation of mean velocities for CFI at a higher computational cost. The Capon method decreased the bias by 81% in the transition band of the used polynomial regression filter for small packet size ( N=8 ) and low SNR (5 dB). Flow phantom and in vivo results demonstrate that the Capon method can provide color flow images and flow profiles with lower variance and bias especially in the regions close to the artery walls.

  8. Adaptive Spectral Estimation Methods in Color Flow Imaging.

    PubMed

    Karabiyik, Yucel; Ekroll, Ingvild Kinn; Eik-Nes, Sturla; Avdal, Jorgen; Lovstakken, Lasse

    2016-07-28

    Clutter rejection for color flow imaging (CFI) remains a challenge due to either limited amount of temporal samples available or non-stationary tissue clutter. This is particularly the case for interleaved CFI and B-mode acquisitions. Low velocity blood signal is attenuated along with the clutter due to the long transition band of the available clutter filters, causing regions of biased mean velocity estimates or signal dropouts. This work investigates how adaptive spectral estimation methods, the Capon and BIAA, can be used to estimate the mean velocity in CFI without prior clutter filtering. The approach is based on confining the clutter signal in a narrow spectral region around the zero Doppler frequency while keeping the spectral side lobes below the blood signal level, allowing for the clutter signal to be removed by thresholding in the frequency domain. The proposed methods are evaluated using computer simulations, flow phantom experiments and in-vivo recordings from the common carotid and jugular vein of healthy volunteers. Capon and BIAA methods could estimate low blood velocities which are normally attenuated by polynomial regression filters, and may potentially give better estimation of mean velocities for CFI at a higher computational cost. The Capon method decreased the bias by 81% in the transition band of the used polynomial regression filter for small packet size (N=8) and low SNR (5 dB). Flow phantom and invivo results demonstrate that the Capon method can provide color flow images and flow profiles with lower variance and bias especially in the regions close to the artery walls.

  9. Simple numerical method for predicting steady compressible flows

    NASA Technical Reports Server (NTRS)

    Vonlavante, Ernst; Nelson, N. Duane

    1986-01-01

    A numerical method for solving the isenthalpic form of the governing equations for compressible viscous and inviscid flows was developed. The method was based on the concept of flux vector splitting in its implicit form. The method was tested on several demanding inviscid and viscous configurations. Two different forms of the implicit operator were investigated. The time marching to steady state was accelerated by the implementation of the multigrid procedure. Its various forms very effectively increased the rate of convergence of the present scheme. High quality steady state results were obtained in most of the test cases; these required only short computational times due to the relative efficiency of the basic method.

  10. Traffic Flow Management Using Aggregate Flow Models and the Development of Disaggregation Methods

    NASA Technical Reports Server (NTRS)

    Sun, Dengfeng; Sridhar, Banavar; Grabbe, Shon

    2010-01-01

    A linear time-varying aggregate traffic flow model can be used to develop Traffic Flow Management (tfm) strategies based on optimization algorithms. However, there are no methods available in the literature to translate these aggregate solutions into actions involving individual aircraft. This paper describes and implements a computationally efficient disaggregation algorithm, which converts an aggregate (flow-based) solution to a flight-specific control action. Numerical results generated by the optimization method and the disaggregation algorithm are presented and illustrated by applying them to generate TFM schedules for a typical day in the U.S. National Airspace System. The results show that the disaggregation algorithm generates control actions for individual flights while keeping the air traffic behavior very close to the optimal solution.

  11. A cell-centered ICE method for multiphase flow simulations

    SciTech Connect

    Kashiwa, B.A.; Padial, N.T.; Rauenzahn, R.M.; VanderHeyden, W.B.

    1993-12-01

    The Implicit Continuous-fluid Eulerian (ICE) method is a finite-volume scheme that is stable for any value of the Courant number based on the sound speed. In the incompressible limit, the ICE method becomes essentially identical to the Marker and Cell (MAC) method, so the two schemes are closely related. In this article, the classical ICE method is extended to multiple interpenetrating phases, and employed with a single control volume (nonstaggered) mesh framework. The incompressible limit is preserved, so that problems involving equations of state, or those exhibiting constant material densities, can be addressed with the same computer code. The scheme reduces properly to a single-fluid method, enabling benchmarking using well-known test cases. Thus, the numerical issues focus only on those aspects unique to problems having multiple density, velocity and temperature fields. The discussion begins with a derivation of the exact, ensemble-averaged equations. Examples of the most basic closures axe given, and the well-posedness of the equations is demonstrated. The numerical method is described in operator notation, and the discretization is sketched. The flow patterns in a bubble column are computed as an incompressible flow example. For a compressible flow example, the expansion and compression of a bubble formed by high-explosive gases under water is shown. In each case, comparison to experimental data is made.

  12. Stochastic Collocation Method for Three-dimensional Groundwater Flow

    NASA Astrophysics Data System (ADS)

    Shi, L.; Zhang, D.

    2008-12-01

    The stochastic collocation method (SCM) has recently gained extensive attention in several disciplines. The numerical implementation of SCM only requires repetitive runs of an existing deterministic solver or code as in the Monte Carlo simulation. But it is generally much more efficient than the Monte Carlo method. In this paper, the stochastic collocation method is used to efficiently qualify uncertainty of three-dimensional groundwater flow. We introduce the basic principles of common collocation methods, i.e., the tensor product collocation method (TPCM), Smolyak collocation method (SmCM), Stround-2 collocation method (StCM), and probability collocation method (PCM). Their accuracy, computational cost, and limitation are discussed. Illustrative examples reveal that the seamless combination of collocation techniques and existing simulators makes the new framework possible to efficiently handle complex stochastic problems.

  13. High Order Filter Methods for Shock/Turbulence MHD Flows

    NASA Technical Reports Server (NTRS)

    Yee, H. C.; Sjoegreen, Bjoern

    2003-01-01

    Low-dissipative high order filter finite difference methods for shock/turbulence/combustion compressible viscous MHD flows has been constructed. Several variants of the filter approach that cater to different flow types are proposed. These filters provide a natural and efficient way for the minimization of the divergence of the magnetic field (del (raided dot) B) numerical error in the sense that no standard divergence cleaning is required. For certain 2-D MHD test problems, divergence free preservation of the magnetic fields of these filter schemes has been achieved.

  14. A multilevel adaptive projection method for unsteady incompressible flow

    NASA Technical Reports Server (NTRS)

    Howell, Louis H.

    1993-01-01

    There are two main requirements for practical simulation of unsteady flow at high Reynolds number: the algorithm must accurately propagate discontinuous flow fields without excessive artificial viscosity, and it must have some adaptive capability to concentrate computational effort where it is most needed. We satisfy the first of these requirements with a second-order Godunov method similar to those used for high-speed flows with shocks, and the second with a grid-based refinement scheme which avoids some of the drawbacks associated with unstructured meshes. These two features of our algorithm place certain constraints on the projection method used to enforce incompressibility. Velocities are cell-based, leading to a Laplacian stencil for the projection which decouples adjacent grid points. We discuss features of the multigrid and multilevel iteration schemes required for solution of the resulting decoupled problem. Variable-density flows require use of a modified projection operator--we have found a multigrid method for this modified projection that successfully handles density jumps of thousands to one. Numerical results are shown for the 2D adaptive and 3D variable-density algorithms.

  15. [Numerical methods for multi-fluid flows]. Final progress report

    SciTech Connect

    Pozrikidis, C.

    1998-07-21

    The central objective of this research has been to develop efficient numerical methods for computing multi-fluid flows with large interfacial deformations, and apply these methods to study the rheology of suspensions of deformable particles with viscous and non-Newtonian interfacial behavior. The mathematical formulation employs boundary-integral, immersed-boundary, and related numerical methods. Particles of interest include liquid drops with constant surface tension and capsules whose interfaces exhibit viscoelastic and incompressible characteristics. In one family of problems, the author has considered the shear-driven and pressure-driven flow of a suspension of two-dimensional liquid drops with ordered and random structure. In a second series of investigations, the author carried out dynamic simulations of two-dimensional, unbounded, doubly-periodic shear flows with random structure. Another family of problems addresses the deformation of three-dimensional capsules whose interfaces exhibit isotropic surface tension, viscous, elastic, or incompressible behavior, in simple shear flow. The numerical results extend previous asymptotic theories for small deformations and illuminate the mechanism of membrane rupture.

  16. Using mixed methods to identify factors influencing patient flow.

    PubMed

    Van Vaerenbergh, Cindy

    2009-11-01

    An effective method of identifying operational factors that influence patient flow can potentially lead to improvements and thus have huge benefits on the efficiency of hospital departments. This paper presents a new inductive mixed-method approach to identify operational factors that influence patient flow through an accident and emergency (A&E) department. Preliminary explorative observations were conducted, followed by semi-structured interviews with key stakeholders. A questionnaire survey of all medical, nursing, porter and clerical staff was then conducted. The observations provided factors for further exploration: skill-mix, long working hours, equipment availability, lack of orientation programmes, inefficient IT use and issues regarding communication structures. Interviewees highlighted several factors, including availability of medical supervision and senior nursing staff, nursing documentation issues, lack of morale due to overcrowding, personality differences and factors relating to the department layout. The questionnaire respondents strongly supported the importance of the previously identified factors. This paper demonstrates an effective mixed-method approach that can be replicated by other health-care managers to identify factors influencing patient flow. Further benefits include increased volume and quality of data, increased staff awareness for the influence of internal factors on patient flow and enhancing the evidence base for future decision making when prioritizing A&E projects.

  17. Intrusive Method for Uncertainty Quantification in a Multiphase Flow Solver

    NASA Astrophysics Data System (ADS)

    Turnquist, Brian; Owkes, Mark

    2016-11-01

    Uncertainty quantification (UQ) is a necessary, interesting, and often neglected aspect of fluid flow simulations. To determine the significance of uncertain initial and boundary conditions, a multiphase flow solver is being created which extends a single phase, intrusive, polynomial chaos scheme into multiphase flows. Reliably estimating the impact of input uncertainty on design criteria can help identify and minimize unwanted variability in critical areas, and has the potential to help advance knowledge in atomizing jets, jet engines, pharmaceuticals, and food processing. Use of an intrusive polynomial chaos method has been shown to significantly reduce computational cost over non-intrusive collocation methods such as Monte-Carlo. This method requires transforming the model equations into a weak form through substitution of stochastic (random) variables. Ultimately, the model deploys a stochastic Navier Stokes equation, a stochastic conservative level set approach including reinitialization, as well as stochastic normals and curvature. By implementing these approaches together in one framework, basic problems may be investigated which shed light on model expansion, uncertainty theory, and fluid flow in general. NSF Grant Number 1511325.

  18. Anisotropic adaptive finite element method for modelling blood flow.

    PubMed

    Müller, J; Sahni, O; Li, X; Jansen, K E; Shephard, M S; Taylor, C A

    2005-10-01

    In this study, we present an adaptive anisotropic finite element method (FEM) and demonstrate how computational efficiency can be increased when applying the method to the simulation of blood flow in the cardiovascular system. We use the SUPG formulation for the transient 3D incompressible Navier-Stokes equations which are discretised by linear finite elements for both the pressure and the velocity field. Given the pulsatile nature of the flow in blood vessels we have pursued adaptivity based on the average flow over a cardiac cycle. Error indicators are derived to define an anisotropic mesh metric field. Mesh modification algorithms are used to anisotropically adapt the mesh according to the desired size field. We demonstrate the efficiency of the method by first applying it to pulsatile flow in a straight cylindrical vessel and then to a porcine aorta with a stenosis bypassed by a graft. We demonstrate that the use of an anisotropic adaptive FEM can result in an order of magnitude reduction in computing time with no loss of accuracy compared to analyses obtained with uniform meshes.

  19. Thermodynamical effects and high resolution methods for compressible fluid flows

    NASA Astrophysics Data System (ADS)

    Li, Jiequan; Wang, Yue

    2017-08-01

    One of the fundamental differences of compressible fluid flows from incompressible fluid flows is the involvement of thermodynamics. This difference should be manifested in the design of numerical schemes. Unfortunately, the role of entropy, expressing irreversibility, is often neglected even though the entropy inequality, as a conceptual derivative, is verified for some first order schemes. In this paper, we refine the GRP solver to illustrate how the thermodynamical variation is integrated into the design of high resolution methods for compressible fluid flows and demonstrate numerically the importance of thermodynamic effects in the resolution of strong waves. As a by-product, we show that the GRP solver works for generic equations of state, and is independent of technical arguments.

  20. Modeling the flow in diffuse interface methods of solidification

    NASA Astrophysics Data System (ADS)

    Subhedar, A.; Steinbach, I.; Varnik, F.

    2015-08-01

    Fluid dynamical equations in the presence of a diffuse solid-liquid interface are investigated via a volume averaging approach. The resulting equations exhibit the same structure as the standard Navier-Stokes equation for a Newtonian fluid with a constant viscosity, the effect of the solid phase fraction appearing in the drag force only. This considerably simplifies the use of the lattice Boltzmann method as a fluid dynamics solver in solidification simulations. Galilean invariance is also satisfied within this approach. Further, we investigate deviations between the diffuse and sharp interface flow profiles via both quasiexact numerical integration and lattice Boltzmann simulations. It emerges from these studies that the freedom in choosing the solid-liquid coupling parameter h provides a flexible way of optimizing the diffuse interface-flow simulations. Once h is adapted for a given spatial resolution, the simulated flow profiles reach an accuracy comparable to quasiexact numerical simulations.

  1. Synthetic-Eddy Method for Urban Atmospheric Flow Modelling

    NASA Astrophysics Data System (ADS)

    Pavlidis, D.; Gorman, G. J.; Gomes, J. L. M. A.; Pain, C. C.; Apsimon, H.

    2010-08-01

    The computational fluid dynamics code Fluidity, with anisotropic mesh adaptivity, is used as a multi-scale obstacle-accommodating meteorological model. A novel method for generating realistic inlet boundary conditions based on the view of turbulence as a superposition of synthetic eddies is adopted. It is able to reproduce prescribed first-order and second-order one-point statistics and turbulence length scales. The aim is to simulate an urban boundary layer. The model is validated against two standard benchmark tests: a plane channel flow numerical simulation and a flow past a cube physical simulation. The performed large-eddy simulations are in good agreement with both reference models giving confidence that the model can be used to successfully simulate urban atmospheric flows.

  2. Vortex flows in the solar chromosphere. I. Automatic detection method

    NASA Astrophysics Data System (ADS)

    Kato, Y.; Wedemeyer, S.

    2017-05-01

    Solar "magnetic tornadoes" are produced by rotating magnetic field structures that extend from the upper convection zone and the photosphere to the corona of the Sun. Recent studies show that these kinds of rotating features are an integral part of atmospheric dynamics and occur on a large range of spatial scales. A systematic statistical study of magnetic tornadoes is a necessary next step towards understanding their formation and their role in mass and energy transport in the solar atmosphere. For this purpose, we develop a new automatic detection method for chromospheric swirls, meaning the observable signature of solar tornadoes or, more generally, chromospheric vortex flows and rotating motions. Unlike existing studies that rely on visual inspections, our new method combines a line integral convolution (LIC) imaging technique and a scalar quantity that represents a vortex flow on a two-dimensional plane. We have tested two detection algorithms, based on the enhanced vorticity and vorticity strength quantities, by applying them to three-dimensional numerical simulations of the solar atmosphere with CO5BOLD. We conclude that the vorticity strength method is superior compared to the enhanced vorticity method in all aspects. Applying the method to a numerical simulation of the solar atmosphere reveals very abundant small-scale, short-lived chromospheric vortex flows that have not been found previously by visual inspection.

  3. Preconditioning methods for ideal and multiphase fluid flows

    NASA Astrophysics Data System (ADS)

    Gupta, Ashish

    The objective of this study is to develop a preconditioning method for ideal and multiphase multispecies compressible fluid flow solver using homogeneous equilibrium mixture model. The mathematical model for fluid flow going through phase change uses density and temperature in the formulation, where the density represents the multiphase mixture density. The change of phase of the fluid is then explicitly determined using the equation of state of the fluid, which only requires temperature and mixture density. The method developed is based on a finite-volume framework in which the numerical fluxes are computed using Roe's approximate Riemann solver and the modified Harten, Lax and Van-leer scheme (HLLC). All speed Roe and HLLC flux based schemes have been developed either by using preconditioning or by directly modifying dissipation to reduce the effect of acoustic speed in its numerical dissipation when Mach number decreases. Preconditioning proposed by Briley, Taylor and Whitfield, Eriksson and Turkel are studied in this research, where as low dissipation schemes proposed by Rieper and Thornber, Mosedale, Drikakis, Youngs and Williams are also considered. Various preconditioners are evaluated in terms of development, performance, accuracy and limitations in simulations at various Mach numbers. A generalized preconditioner is derived which possesses well conditioned eigensystem for multiphase multispecies flow simulations. Validation and verification of the solution procedure are carried out on several small model problems with comparison to experimental, theoretical, and other numerical results. Preconditioning methods are evaluated using three basic geometries; 1) bump in a channel 2) flow over a NACA0012 airfoil and 3) flow over a cylinder, which are then compared with theoretical and numerical results. Multiphase capabilities of the solver are evaluated in cryogenic and non-cryogenic conditions. For cryogenic conditions the solver is evaluated by predicting

  4. Method for Studying Microbial Biofilms in Flowing-Water Systems

    PubMed Central

    Pedersen, Karsten

    1982-01-01

    A method for the study of microbial biofilms in flowing-water systems was developed with special reference to the flow conditions in electrochemical concentration cells. Seawater was circulated in a semiclosed flow system through biofilm reactors (3 cm s−1) with microscope cover slips arranged in lamellar piles parallel with the flow. At fixed time intervals cover slips with their biofilm were removed from the pile, stained with crystal violet, and mounted on microscope slides. The absorbances of the slides were measured at 590 nm and plotted against time to give microbial biofilm development. From calibration experiments a staining time of 1 min and a rinse time of 10 min in a tap water flow (3 cm s−1) were considered sufficient. When an analysis of variance was performed on biofilm development data, 78% of the total variance was found to be due to random natural effects; the rest could be explained by experimental effects. The absorbance values correlated well with protein N, dry weight, and organic weight in two biofilm experiments, one with a biofilm with a high (75%) and one with a low (∼25%, normal) inorganic content. Comparisons of regression lines revealed that the absorbance of the stained biofilms was an estimate closely related to biofilm dry weight. PMID:16345929

  5. Flow visualisation of downhill skiers using the lattice Boltzmann method

    NASA Astrophysics Data System (ADS)

    Asai, Takeshi; Hong, Sungchan; Ijuin, Koichi

    2017-03-01

    In downhill alpine skiing, skiers often exceed speeds of 120 km h-1, with air resistance substantially affecting the overall race times. To date, studies on air resistance in alpine skiing have used wind tunnels and actual skiers to examine the relationship between the gliding posture and magnitude of drag and for the design of skiing equipment. However, these studies have not revealed the flow velocity distribution and vortex structure around the skier. In the present study, computational fluid dynamics are employed with the lattice Boltzmann method to derive the relationship between total drag and the flow velocity around a downhill skier in the full-tuck position. Furthermore, the flow around the downhill skier is visualised, and its vortex structure is examined. The results show that the total drag force in the downhill skier model is 27.0 N at a flow velocity of 15 m s-1, increasing to 185.8 N at 40 m s-1. From analysis of the drag distribution and the flow profile, the head, upper arms, lower legs, and thighs (including buttocks) are identified as the major sources of drag on a downhill skier. Based on these results, the design of suits and equipment for reducing the drag from each location should be the focus of research and development in ski equipment. This paper describes a pilot study that introduces undergraduate students of physics or engineering into this research field. The results of this study are easy to understand for undergraduate students.

  6. Numerical Methods and Simulations of Complex Multiphase Flows

    NASA Astrophysics Data System (ADS)

    Brady, Peter

    Multiphase flows are an important part of many natural and technological phenomena such as ocean-air coupling (which is important for climate modeling) and the atomization of liquid fuel jets in combustion engines. The unique challenges of multiphase flow often make analytical solutions to the governing equations impossible and experimental investigations very difficult. Thus, high-fidelity numerical simulations can play a pivotal role in understanding these systems. This dissertation describes numerical methods developed for complex multiphase flows and the simulations performed using these methods. First, the issue of multiphase code verification is addressed. Code verification answers the question "Is this code solving the equations correctly?" The method of manufactured solutions (MMS) is a procedure for generating exact benchmark solutions which can test the most general capabilities of a code. The chief obstacle to applying MMS to multiphase flow lies in the discontinuous nature of the material properties at the interface. An extension of the MMS procedure to multiphase flow is presented, using an adaptive marching tetrahedron style algorithm to compute the source terms near the interface. Guidelines for the use of the MMS to help locate coding mistakes are also detailed. Three multiphase systems are then investigated: (1) the thermocapillary motion of three-dimensional and axisymmetric drops in a confined apparatus, (2) the flow of two immiscible fluids completely filling an enclosed cylinder and driven by the rotation of the bottom endwall, and (3) the atomization of a single drop subjected to a high shear turbulent flow. The systems are simulated numerically by solving the full multiphase Navier-Stokes equations coupled to the various equations of state and a level set interface tracking scheme based on the refined level set grid method. The codes have been parallelized using MPI in order to take advantage of today's very large parallel computational

  7. Pseudo-compressibility methods for the incompressible flow equations

    NASA Technical Reports Server (NTRS)

    Turkel, Eli; Arnone, A.

    1993-01-01

    Preconditioning methods to accelerate convergence to a steady state for the incompressible fluid dynamics equations are considered. The analysis relies on the inviscid equations. The preconditioning consists of a matrix multiplying the time derivatives. Thus the steady state of the preconditioned system is the same as the steady state of the original system. The method is compared to other types of pseudo-compressibility. For finite difference methods preconditioning can change and improve the steady state solutions. An application to viscous flow around a cascade with a non-periodic mesh is presented.

  8. A conservative method for hydrostatic flow in isentropic coordinates

    NASA Astrophysics Data System (ADS)

    Peeters, B.; Bokhove, O.; Frank, J.

    2010-05-01

    Although our climate is ultimately driven by (nonuniform) solar heating, many aspects of the flow can be understood qualitatively from forcing-free and frictionless dynamics. In the limit of zero forcing and dissipation, our weather system falls under the realm of Hamiltonian fluid dynamics and the flow conserves potential vorticity (PV), energy and phase-space structure. We have found a conservative numerical scheme for a hydrostatic atmosphere based on a mixed Eulerian-Lagrangian approach, the so-called parcel formulation [1]. For adiabatic flow, the entropy is materially conserved. Under stable stratifications, we introduce isentropic coordinates to simplify the governing equations. The entropic direction is discretized using finite elements. The discretization of horizontal Lagrangian label space (from infinitesimal fluid parcels to discrete fluid particles) yields a discrete Poisson bracket. New is that we apply the Hamiltonian Particle-Mesh method [2], and view the potential as an Eulerian function, reconstructed from the particle data. The use of an Eulerian grid makes the method more efficient and stable. The Hamiltonian consists of a Lagrangian kinetic energy and an Eulerian potential energy. The discrete system of ODE's is thus a Hamiltonian system conserving mass, PV, energy and phase-space structure. If we incorporate a symplectic time integrator, the resulting fully discrete system conserves energy approximately without any drift in energy. Several challenging (nonlinear) solutions will be tested, such a flow over a rising bump. Also, preliminary results for bottom-intersecting isentropes will be demonstrated. REFERENCES [1] O. Bokhove and M. Oliver, Parcel Eulerian-Lagrangian fluid dynamics for rotating geophysical flows, Proc. Roy. Soc. A. 462, pp. 2563-2573 (2006) [2] J. Frank, G. Gottwald, S. Reich, A Hamiltonian particle-mesh method for the rotating shallow-water equations, Lecture Notes in Computational Science and Engineering, Vol. 26, Springer

  9. A spectral boundary integral method for flowing blood cells

    NASA Astrophysics Data System (ADS)

    Zhao, Hong; Isfahani, Amir H. G.; Olson, Luke N.; Freund, Jonathan B.

    2010-05-01

    A spectral boundary integral method for simulating large numbers of blood cells flowing in complex geometries is developed and demonstrated. The blood cells are modeled as finite-deformation elastic membranes containing a higher viscosity fluid than the surrounding plasma, but the solver itself is independent of the particular constitutive model employed for the cell membranes. The surface integrals developed for solving the viscous flow, and thereby the motion of the massless membrane, are evaluated using an O(NlogN) particle-mesh Ewald (PME) approach. The cell shapes, which can become highly distorted under physiologic conditions, are discretized with spherical harmonics. The resolution of these global basis functions is, of course, excellent, but more importantly they facilitate an approximate de-aliasing procedure that stabilizes the simulations without adding any numerical dissipation or further restricting the permissible numerical time step. Complex geometry no-slip boundaries are included using a constraint method that is coupled into an implicit system that is solved as part of the time advancement routine. The implementation is verified against solutions for axisymmetric flows reported in the literature, and its accuracy is demonstrated by comparison against exact solutions for relaxing surface deformations. It is also used to simulate flow of blood cells at 30% volume fraction in tubes between 4.9 and 16.9 μm in diameter. For these, it is shown to reproduce the well-known non-monotonic dependence of the effective viscosity on the tube diameter.

  10. The new performance calculation method of fouled axial flow compressor.

    PubMed

    Yang, Huadong; Xu, Hong

    2014-01-01

    Fouling is the most important performance degradation factor, so it is necessary to accurately predict the effect of fouling on engine performance. In the previous research, it is very difficult to accurately model the fouled axial flow compressor. This paper develops a new performance calculation method of fouled multistage axial flow compressor based on experiment result and operating data. For multistage compressor, the whole compressor is decomposed into two sections. The first section includes the first 50% stages which reflect the fouling level, and the second section includes the last 50% stages which are viewed as the clean stage because of less deposits. In this model, the performance of the first section is obtained by combining scaling law method and linear progression model with traditional stage stacking method; simultaneously ambient conditions and engine configurations are considered. On the other hand, the performance of the second section is calculated by averaged infinitesimal stage method which is based on Reynolds' law of similarity. Finally, the model is successfully applied to predict the 8-stage axial flow compressor and 16-stage LM2500-30 compressor. The change of thermodynamic parameters such as pressure ratio, efficiency with the operating time, and stage number is analyzed in detail.

  11. The New Performance Calculation Method of Fouled Axial Flow Compressor

    PubMed Central

    Xu, Hong

    2014-01-01

    Fouling is the most important performance degradation factor, so it is necessary to accurately predict the effect of fouling on engine performance. In the previous research, it is very difficult to accurately model the fouled axial flow compressor. This paper develops a new performance calculation method of fouled multistage axial flow compressor based on experiment result and operating data. For multistage compressor, the whole compressor is decomposed into two sections. The first section includes the first 50% stages which reflect the fouling level, and the second section includes the last 50% stages which are viewed as the clean stage because of less deposits. In this model, the performance of the first section is obtained by combining scaling law method and linear progression model with traditional stage stacking method; simultaneously ambient conditions and engine configurations are considered. On the other hand, the performance of the second section is calculated by averaged infinitesimal stage method which is based on Reynolds' law of similarity. Finally, the model is successfully applied to predict the 8-stage axial flow compressor and 16-stage LM2500-30 compressor. The change of thermodynamic parameters such as pressure ratio, efficiency with the operating time, and stage number is analyzed in detail. PMID:25197717

  12. Smoothed particle hydrodynamics method for evaporating multiphase flows

    NASA Astrophysics Data System (ADS)

    Yang, Xiufeng; Kong, Song-Charng

    2017-09-01

    The smoothed particle hydrodynamics (SPH) method has been increasingly used for simulating fluid flows; however, its ability to simulate evaporating flow requires significant improvements. This paper proposes an SPH method for evaporating multiphase flows. The present SPH method can simulate the heat and mass transfers across the liquid-gas interfaces. The conservation equations of mass, momentum, and energy were reformulated based on SPH, then were used to govern the fluid flow and heat transfer in both the liquid and gas phases. The continuity equation of the vapor species was employed to simulate the vapor mass fraction in the gas phase. The vapor mass fraction at the interface was predicted by the Clausius-Clapeyron correlation. An evaporation rate was derived to predict the mass transfer from the liquid phase to the gas phase at the interface. Because of the mass transfer across the liquid-gas interface, the mass of an SPH particle was allowed to change. Alternative particle splitting and merging techniques were developed to avoid large mass difference between SPH particles of the same phase. The proposed method was tested by simulating three problems, including the Stefan problem, evaporation of a static drop, and evaporation of a drop impacting a hot surface. For the Stefan problem, the SPH results of the evaporation rate at the interface agreed well with the analytical solution. For drop evaporation, the SPH result was compared with the result predicted by a level-set method from the literature. In the case of drop impact on a hot surface, the evolution of the shape of the drop, temperature, and vapor mass fraction were predicted.

  13. Unstructured Mesh Methods for the Simulation of Hypersonic Flows

    NASA Technical Reports Server (NTRS)

    Peraire, Jaime; Bibb, K. L. (Technical Monitor)

    2001-01-01

    This report describes the research work undertaken at the Massachusetts Institute of Technology. The aim of this research is to identify effective algorithms and methodologies for the efficient and routine solution of hypersonic viscous flows about re-entry vehicles. For over ten years we have received support from NASA to develop unstructured mesh methods for Computational Fluid Dynamics. As a result of this effort a methodology based on the use, of unstructured adapted meshes of tetrahedra and finite volume flow solvers has been developed. A number of gridding algorithms flow solvers, and adaptive strategies have been proposed. The most successful algorithms developed from the basis of the unstructured mesh system FELISA. The FELISA system has been extensively for the analysis of transonic and hypersonic flows about complete vehicle configurations. The system is highly automatic and allows for the routine aerodynamic analysis of complex configurations starting from CAD data. The code has been parallelized and utilizes efficient solution algorithms. For hypersonic flows, a version of the, code which incorporates real gas effects, has been produced. One of the latest developments before the start of this grant was to extend the system to include viscous effects. This required the development of viscous generators, capable of generating the anisotropic grids required to represent boundary layers, and viscous flow solvers. In figures I and 2, we show some sample hypersonic viscous computations using the developed viscous generators and solvers. Although these initial results were encouraging, it became apparent that in order to develop a fully functional capability for viscous flows, several advances in gridding, solution accuracy, robustness and efficiency were required. As part of this research we have developed: 1) automatic meshing techniques and the corresponding computer codes have been delivered to NASA and implemented into the GridEx system, 2) a finite

  14. Axial flow heat exchanger devices and methods for heat transfer using axial flow devices

    DOEpatents

    Koplow, Jeffrey P.

    2016-02-16

    Systems and methods described herein are directed to rotary heat exchangers configured to transfer heat to a heat transfer medium flowing in substantially axial direction within the heat exchangers. Exemplary heat exchangers include a heat conducting structure which is configured to be in thermal contact with a thermal load or a thermal sink, and a heat transfer structure rotatably coupled to the heat conducting structure to form a gap region between the heat conducting structure and the heat transfer structure, the heat transfer structure being configured to rotate during operation of the device. In example devices heat may be transferred across the gap region from a heated axial flow of the heat transfer medium to a cool stationary heat conducting structure, or from a heated stationary conducting structure to a cool axial flow of the heat transfer medium.

  15. Computational flow development for unsteady viscous flows: Foundation of the numerical method

    NASA Technical Reports Server (NTRS)

    Bratanow, T.; Spehert, T.

    1978-01-01

    A procedure is presented for effective consideration of viscous effects in computational development of high Reynolds number flows. The procedure is based on the interpretation of the Navier-Stokes equations as vorticity transport equations. The physics of the flow was represented in a form suitable for numerical analysis. Lighthill's concept for flow development for computational purposes was adapted. The vorticity transport equations were cast in a form convenient for computation. A statement for these equations was written using the method of weighted residuals and applying the Galerkin criterion. An integral representation of the induced velocity was applied on the basis of the Biot-Savart law. Distribution of new vorticity, produced at wing surfaces over small computational time intervals, was assumed to be confined to a thin region around the wing surfaces.

  16. Multiple light scattering methods for multiphase flow diagnostics

    NASA Astrophysics Data System (ADS)

    Estevadeordal, Jordi

    2015-11-01

    Multiphase flows of gases and liquids containing droplets, bubbles, or particulates present light scattering imaging challenges due to the interference from each phase, such as secondary reflections, extinctions, absorptions, and refractions. These factors often prevent the unambiguous detection of each phase and also produce undesired beam steering. The effects can be especially complex in presence of dense phases, multispecies flows, and high pressure environments. This investigation reports new methods for overcoming these effects for quantitative measurements of velocity, density, and temperature fields. The methods are based on light scattering techniques combining Mie and filtered Rayleigh scattering and light extinction analyses and measurements. The optical layout is designed to perform multiple property measurements with improved signal from each phase via laser spectral and polarization characterization, etalon decontamination, and use of multiple wavelengths and imaging detectors.

  17. Silver and gold enhancement methods for lateral flow immunoassays.

    PubMed

    Rodríguez, Myriam Oliveira; Covián, Lucía Blanco; García, Agustín Costa; Blanco-López, Maria Carmen

    2016-01-01

    Sensitivity is the main concern at the development of rapid test by lateral flow immunoassays. On the other hand, low limits of detection are often required at medical diagnostics and other field of analysis. To overcome this drawback, several enhancement protocols have been described. In this paper, we have selected different silver enhancement methods and one dual gold conjugation, and we critically compared the amplification produced when applied to a gold-nanoparticle based lateral flow immunoassay for the detection of prostate specific antigen (PSA). The highest amplification was obtained by using an immersion method based on a solution of silver nitrate and hydroquinone/citrate buffer in proportion 1:1. Under these conditions, the system is capable of detecting PSA within 20 min at levels as low as 0.1 ng/mL, with a 3-fold sensitivity improvement. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Aerosol flow reactor method for synthesis of drug nanoparticles.

    PubMed

    Eerikäinen, Hannele; Watanabe, Wiwik; Kauppinen, Esko I; Ahonen, P Petri

    2003-05-01

    An aerosol flow reactor method, a one-step continuous process to produce nanometer-sized drug particles with unimodal size distribution, was developed. This method involves first dissolving the drug material in question into a suitable solvent, which is then followed by atomising the solution as fine droplets into carrier gas. A heated laminar flow reactor tube is used to evaporate the solvent, and solid drug nanoparticles are formed. In this study, the effect of drying temperature on the particle size and morphology was examined. A glucocorticosteroid used for asthma therapy, beclomethasone dipropionate, was selected as an experimental model drug. The geometric number mean particle diameter increases significantly with increasing reactor temperatures due to formation of hollow nanoparticles. Above 160 degrees C, however, further increase in temperature results in decreasing particle size. The produced nanoparticles are spherical and show smooth surfaces at all studied experimental conditions.

  19. Preconditioned lattice-Boltzmann method for steady flows

    NASA Astrophysics Data System (ADS)

    Guo, Zhaoli; Zhao, T. S.; Shi, Yong

    2004-12-01

    In this paper we propose a preconditioned lattice Boltzmann (LB) method for steady incompressible flows. For steady flows, the macroscopic equations derived from this LB model are equivalent to those from the standard LB model, but with an improved eigenvalue system. The proposed model can be viewed as an explicit solver for preconditioned compressible Navier-Stokes equations. Linear stability analysis is performed and the results show that the stability of the model is the same as that of the standard LB model for low Mach numbers. The proposed model retains the structure of the standard LB model and, hence, possesses all the advantages. Numerical tests show that the convergence rate can be enhanced as much as an order of magnitude compared to the standard lattice Boltzmann method. The accuracy of the solutions is improved as well.

  20. Research on stochastic power-flow study methods. Final report

    SciTech Connect

    Heydt, G. T.

    1981-01-01

    A general algorithm to determine the effects of uncertainty in bus load and generation on the output of conventional power flow analysis is presented. The use of statistical moments is presented and developed as a means for representing the stochastic process. Statistical moments are used to describe the uncertainties, and facilitate the calculations of single and multivarlate probability density functions of input and output variables. The transformation of the uncertainty through the power flow equations is made by the expansion of the node equations in a multivariate Taylor series about an expected operating point. The series is truncated after the second order terms. Since the power flow equations are nonlinear, the expected values of output quantities is in general not the solution to the conventional load flow problem using expected values of input quantities. The second order transformation offers a correction vector and allows the consideration of larger uncertainties which have caused significant error in the current linear transformation algorithms. Voltage controlled busses are included with consideration of upper and lower limits. The finite reactive power available at generation sites, and fixed ranges of transformer tap movement may have a significant effect on voltage and line power flow statistics. A method is given which considers limitation constraints in the evaluation of all output quantities. The bus voltages, line power flows, transformer taps, and generator reactive power requirements are described by their statistical moments. Their values are expressed in terms of the probability that they are above or below specified limits, and their expected values given that they do fall outside the limits. Thus the algorithm supplies information about severity of overload as well as probability of occurrence. An example is given for an eleven bus system, evaluating each quantity separately. The results are compared with Monte Carlo simulation.

  1. High speed inviscid compressible flow by the finite element method

    NASA Technical Reports Server (NTRS)

    Zienkiewicz, O. C.; Loehner, R.; Morgan, K.

    1984-01-01

    The finite element method and an explicit time stepping algorithm which is based on Taylor-Galerkin schemes with an appropriate artificial viscosity is combined with an automatic mesh refinement process which is designed to produce accurate steady state solutions to problems of inviscid compressible flow in two dimensions. The results of two test problems are included which demonstrate the excellent performance characteristics of the proposed procedures.

  2. Method and apparatus for continuous flow injection extraction analysis

    DOEpatents

    Hartenstein, Steven D.; Siemer, Darryl D.

    1992-01-01

    A method and apparatus for a continuous flow injection batch extraction aysis system is disclosed employing extraction of a component of a first liquid into a second liquid which is a solvent for a component of the first liquid, and is immiscible with the first liquid, and for separating the first liquid from the second liquid subsequent to extraction of the component of the first liquid.

  3. Development of Methods for Diagnostics of Discharges in Supersonic Flows

    DTIC Science & Technology

    2004-03-01

    flow, diagnostic methods, electric probe, measurement circuit, propane-air mixture, ignition. Participating Institution : Department of...932-88-20 E-mail: dean@phys.msu.su /ershov@ph-elec.phys.msu.su Partner: The European Office of Aerospace Research and Development (EOARD) Adress...of flight . The gas discharges of a various type can be the perspective tool of the decision of this problem. The definition of the most effective

  4. Mathematical aspects of finite element methods for incompressible viscous flows

    NASA Technical Reports Server (NTRS)

    Gunzburger, M. D.

    1986-01-01

    Mathematical aspects of finite element methods are surveyed for incompressible viscous flows, concentrating on the steady primitive variable formulation. The discretization of a weak formulation of the Navier-Stokes equations are addressed, then the stability condition is considered, the satisfaction of which insures the stability of the approximation. Specific choices of finite element spaces for the velocity and pressure are then discussed. Finally, the connection between different weak formulations and a variety of boundary conditions is explored.

  5. Lagrangian transported MDF methods for compressible high speed flows

    NASA Astrophysics Data System (ADS)

    Gerlinger, Peter

    2017-06-01

    This paper deals with the application of thermochemical Lagrangian MDF (mass density function) methods for compressible sub- and supersonic RANS (Reynolds Averaged Navier-Stokes) simulations. A new approach to treat molecular transport is presented. This technique on the one hand ensures numerical stability of the particle solver in laminar regions of the flow field (e.g. in the viscous sublayer) and on the other hand takes differential diffusion into account. It is shown in a detailed analysis, that the new method correctly predicts first and second-order moments on the basis of conventional modeling approaches. Moreover, a number of challenges for MDF particle methods in high speed flows is discussed, e.g. high cell aspect ratio grids close to solid walls, wall heat transfer, shock resolution, and problems from statistical noise which may cause artificial shock systems in supersonic flows. A Mach 2 supersonic mixing channel with multiple shock reflection and a model rocket combustor simulation demonstrate the eligibility of this technique to practical applications. Both test cases are simulated successfully for the first time with a hybrid finite-volume (FV)/Lagrangian particle solver (PS).

  6. A multigrid nonoscillatory method for computing high speed flows

    NASA Technical Reports Server (NTRS)

    Li, C. P.; Shieh, T. H.

    1993-01-01

    A multigrid method using different smoothers has been developed to solve the Euler equations discretized by a nonoscillatory scheme up to fourth order accuracy. The best smoothing property is provided by a five-stage Runge-Kutta technique with optimized coefficients, yet the most efficient smoother is a backward Euler technique in factored and diagonalized form. The singlegrid solution for a hypersonic, viscous conic flow is in excellent agreement with the solution obtained by the third order MUSCL and Roe's method. Mach 8 inviscid flow computations for a complete entry probe have shown that the accuracy is at least as good as the symmetric TVD scheme of Yee and Harten. The implicit multigrid method is four times more efficient than the explicit multigrid technique and 3.5 times faster than the single-grid implicit technique. For a Mach 8.7 inviscid flow over a blunt delta wing at 30 deg incidence, the CPU reduction factor from the three-level multigrid computation is 2.2 on a grid of 37 x 41 x 73 nodes.

  7. Modeling metal stocks and flows: a review of dynamic material flow analysis methods.

    PubMed

    Müller, Esther; Hilty, Lorenz M; Widmer, Rolf; Schluep, Mathias; Faulstich, Martin

    2014-02-18

    Dynamic material flow analysis (MFA) is a frequently used method to assess past, present, and future stocks and flows of metals in the anthroposphere. Over the past fifteen years, dynamic MFA has contributed to increased knowledge about the quantities, qualities, and locations of metal-containing goods. This article presents a literature review of the methodologies applied in 60 dynamic MFAs of metals. The review is based on a standardized model description format, the ODD (overview, design concepts, details) protocol. We focus on giving a comprehensive overview of modeling approaches and structure them according to essential aspects, such as their treatment of material dissipation, spatial dimension of flows, or data uncertainty. The reviewed literature features similar basic modeling principles but very diverse extrapolation methods. Basic principles include the calculation of outflows of the in-use stock based on inflow or stock data and a lifetime distribution function. For extrapolating stocks and flows, authors apply constant, linear, exponential, and logistic models or approaches based on socioeconomic variables, such as regression models or the intensity-of-use hypothesis. The consideration and treatment of further aspects, such as dissipation, spatial distribution, and data uncertainty, vary significantly and highly depends on the objectives of each study.

  8. Newton like: Minimal residual methods applied to transonic flow calculations

    NASA Technical Reports Server (NTRS)

    Wong, Y. S.

    1984-01-01

    A computational technique for the solution of the full potential equation is presented. The method consists of outer and inner iterations. The outer iterate is based on a Newton like algorithm, and a preconditioned Minimal Residual method is used to seek an approximate solution of the system of linear equations arising at each inner iterate. The present iterative scheme is formulated so that the uncertainties and difficulties associated with many iterative techniques, namely the requirements of acceleration parameters and the treatment of additional boundary conditions for the intermediate variables, are eliminated. Numerical experiments based on the new method for transonic potential flows around the NACA 0012 airfoil at different Mach numbers and different angles of attack are presented, and these results are compared with those obtained by the Approximate Factorization technique. Extention to three dimensional flow calculations and application in finite element methods for fluid dynamics problems by the present method are also discussed. The Inexact Newton like method produces a smoother reduction in the residual norm, and the number of supersonic points and circulations are rapidly established as the number of iterations is increased.

  9. Simulation of dry granular flows using discrete element methods

    NASA Astrophysics Data System (ADS)

    Martin, Hugo; Lefebvre, Aline; Maday, Yvon; Mangeney, Anne; Maury, Bertrand; Sainte-Marie, Jacques

    2017-04-01

    Granular flows are composed of interacting particles (for instance sand grains). While natural flow simulations at the field scale are generally based on continuum models, discrete element methods are very useful to get insight into the detailed contact interactions between the particles involved. We shall consider here both well known molecular dynamics (MD) and contact dynamics (CD) methods to simulate granular particle interaction. The difference between these methods is the linearisation of contact forces in MD. We are interested to compare these methods, and especially the effects of the linearisation in simulations. In the present work, we introduce a new rigid bodies model at the scale of the particles and its resolution by contact dynamics. The interesting aspect of our CD method is to treat the contacts in all the material system in one step without any iterative process required when the contacts are dealt with one after the other. All contacts are calculated here at the same time in just one iteration and the normal and tangential constraints are treated simultaneously. The present model follows from a convex optimization problem presented in [1] by B. Maury in which we add a frictional behaviour to the contact law between the particles. To analyse the behaviour of this model, we compare our results to analytical solutions when we can compute them and otherwise to simulations with molecular dynamics method. [1] A time-stepping scheme for inelastic collisions. Numerical handling of the nonoverlapping constraint, B. Maury, Numerische Mathematik, 17 january 2006.

  10. Path planning in uncertain flow fields using ensemble method

    NASA Astrophysics Data System (ADS)

    Wang, Tong; Le Maître, Olivier P.; Hoteit, Ibrahim; Knio, Omar M.

    2016-10-01

    An ensemble-based approach is developed to conduct optimal path planning in unsteady ocean currents under uncertainty. We focus our attention on two-dimensional steady and unsteady uncertain flows, and adopt a sampling methodology that is well suited to operational forecasts, where an ensemble of deterministic predictions is used to model and quantify uncertainty. In an operational setting, much about dynamics, topography, and forcing of the ocean environment is uncertain. To address this uncertainty, the flow field is parametrized using a finite number of independent canonical random variables with known densities, and the ensemble is generated by sampling these variables. For each of the resulting realizations of the uncertain current field, we predict the path that minimizes the travel time by solving a boundary value problem (BVP), based on the Pontryagin maximum principle. A family of backward-in-time trajectories starting at the end position is used to generate suitable initial values for the BVP solver. This allows us to examine and analyze the performance of the sampling strategy and to develop insight into extensions dealing with general circulation ocean models. In particular, the ensemble method enables us to perform a statistical analysis of travel times and consequently develop a path planning approach that accounts for these statistics. The proposed methodology is tested for a number of scenarios. We first validate our algorithms by reproducing simple canonical solutions, and then demonstrate our approach in more complex flow fields, including idealized, steady and unsteady double-gyre flows.

  11. An improved higher order panel method for linearized supersonic flow

    NASA Technical Reports Server (NTRS)

    Ehlers, F. E.; Epton, M. A.; Johnson, F. T.; Magnus, A. E.; Rubbert, P. E.

    1978-01-01

    An improved higher order panel method for linearized supersonic flow is described. Each panel, defined by four points on the surface, is divided into eight subpanels in such a way that all subpanel and panel edges are contiguous. By prescribing a quadratic distribution of the doublet on each subpanel, the doublet strength is made strictly continuous on the paneled surface. A linear source distribution is also used. Numerical results are smoother and in better agreement with experiment than the previous method with less strict continuity. A brief discussion of superinclined panels used to eliminate interior interference in nacelles is included.

  12. High Order Accuracy Methods for Supersonic Reactive Flows

    DTIC Science & Technology

    2008-06-25

    bubble interactions [12], the supersonic cavity flows [11], and etc. The difficulty of implement - ing the spectral method to these complex fluid...enhance the convergence properties of the approximation via a filter function σ(η) [30] with the following properties σ(η)= σ(−η), σ(±1) = 0, σ(0)= 1, σ... implementation of the Hybrid method depends on the ability to obtain accurate information on the smoothness of a function . In this work, we employ the Multi

  13. Selective flow path alpha particle detector and method of use

    DOEpatents

    Orr, Christopher Henry; Luff, Craig Janson; Dockray, Thomas; Macarthur, Duncan Whittemore

    2002-01-01

    A method and apparatus for monitoring alpha contamination are provided in which ions generated in the air surrounding the item, by the passage of alpha particles, are moved to a distant detector location. The parts of the item from which ions are withdrawn can be controlled by restricting the air flow over different portions of the apparatus. In this way, detection of internal and external surfaces separately, for instance, can be provided. The apparatus and method are particularly suited for use in undertaking alpha contamination measurements during the commissioning operations.

  14. An improved higher order panel method for linearized supersonic flow

    NASA Technical Reports Server (NTRS)

    Ehlers, F. E.; Epton, M. A.; Johnson, F. T.; Magnus, A. E.; Rubbert, P. E.

    1978-01-01

    An improved higher order panel method for linearized supersonic flow is described. Each panel, defined by four points on the surface, is divided into eight subpanels in such a way that all subpanel and panel edges are contiguous. By prescribing a quadratic distribution of the doublet on each subpanel, the doublet strength is made strictly continuous on the paneled surface. A linear source distribution is also used. Numerical results are smoother and in better agreement with experiment than the previous method with less strict continuity. A brief discussion of superinclined panels used to eliminate interior interference in nacelles is included.

  15. Numerical Method for Darcy Flow Derived Using Discrete Exterior Calculus

    NASA Astrophysics Data System (ADS)

    Hirani, A. N.; Nakshatrala, K. B.; Chaudhry, J. H.

    2015-05-01

    We derive a numerical method for Darcy flow, and also for Poisson's equation in mixed (first order) form, based on discrete exterior calculus (DEC). Exterior calculus is a generalization of vector calculus to smooth manifolds and DEC is one of its discretizations on simplicial complexes such as triangle and tetrahedral meshes. DEC is a coordinate invariant discretization, in that it does not depend on the embedding of the simplices or the whole mesh. We start by rewriting the governing equations of Darcy flow using the language of exterior calculus. This yields a formulation in terms of flux differential form and pressure. The numerical method is then derived by using the framework provided by DEC for discretizing differential forms and operators that act on forms. We also develop a discretization for a spatially dependent Hodge star that varies with the permeability of the medium. This also allows us to address discontinuous permeability. The matrix representation for our discrete non-homogeneous Hodge star is diagonal, with positive diagonal entries. The resulting linear system of equations for flux and pressure are saddle type, with a diagonal matrix as the top left block. The performance of the proposed numerical method is illustrated on many standard test problems. These include patch tests in two and three dimensions, comparison with analytically known solutions in two dimensions, layered medium with alternating permeability values, and a test with a change in permeability along the flow direction. We also show numerical evidence of convergence of the flux and the pressure. A convergence experiment is included for Darcy flow on a surface. A short introduction to the relevant parts of smooth and discrete exterior calculus is included in this article. We also include a discussion of the boundary condition in terms of exterior calculus.

  16. Detection of Significant Bacteriuria by Automated Urinalysis Using Flow Cytometry

    PubMed Central

    Okada, Hiroshi; Sakai, Yutaka; Miyazaki, Shigenori; Arakawa, Soichi; Hamaguchi, Yukio; Kamidono, Sadao

    2000-01-01

    A new flow cytometry-based automated urine analyzer, the UF-50, was evaluated for its ability to screen urine samples for significant bacteriuria. One hundred eighty-six urine specimens from patients attending an outpatient clinic of a university-based hospital were examined. The results obtained with the UF-50 were compared with those obtained by conventional quantitative urine culture. The UF-50 detected significant bacteriuria with a sensitivity of 83.1%, a specificity of 76.4%, a positive predictive value of 62.0%, a negative predictive value of 90.7%, and an accuracy of 78.5%. These results are comparable to those obtained by previously reported screening procedures. Besides detecting significant bacteriuria, the UF-50 can also perform routine urinalysis, including measurement of concentrations of red blood cells, white blood cells, epithelial cells, and casts, within 70 s. This capability renders this new flow cytometry-based urine analyzer superior to previously reported rapid screening methods. PMID:10921941

  17. Heat transfer in internal turbulent flows using the PDF method

    SciTech Connect

    Mazumder, S.; Modest, M.F.

    1996-12-31

    One of the strengths of the velocity-composition joint probability density function (PDF) method lies in its ability to predict scalar fields for reactive turbulent flows. The application of PDF methods to internal flows necessitates appropriate description of near-wall effects, namely, molecular transport, production of turbulence by inhomogeneities, and dissipation of the scalar fluctuations by viscosity. A Lagrangian transport equation has been derived for transport of energy, whereby convection is treated exactly. The temperature fluctuations are modeled by a modified version of a deterministic model, which was originally developed for homogeneous turbulence. The thermal wall-functions were used to incorporate these modifications. The resultant modeled Lagrangian energy transport equation is solved simultaneously with the hydrodynamic equations, for the test case of a thermally developing two-dimensional channel flow (parallel plate geometry). The model has been tested for both constant temperature and constant heat flux boundary conditions. Results obtained have been compared to {kappa}-{epsilon} and algebraic Reynolds stress model (ARSM) finite-volume calculations. Apart from the differences due to turbulence models, it was observed that the finite-volume calculations suffered numerical diffusion, which was completely eliminated in the Lagrangian PDF approach.

  18. Solution of plane cascade flow using improved surface singularity methods. [application of panel method to internal aerodynamics

    NASA Technical Reports Server (NTRS)

    Mcfarland, E. R.

    1981-01-01

    A solution method was developed for calculating compressible inviscid flow through a linear cascade of arbitrary blade shapes. The method uses advanced surface singularity formulations which were adapted from those in current external flow analyses. The resulting solution technique provides a fast flexible calculation for flows through turbomachinery blade rows. The solution method and some examples of the method's capabilities are presented.

  19. Higher order time integration methods for two-phase flow

    NASA Astrophysics Data System (ADS)

    Kees, Christopher E.; Miller, Cass T.

    Time integration methods that adapt in both the order of approximation and time step have been shown to provide efficient solutions to Richards' equation. In this work, we extend the same method of lines approach to solve a set of two-phase flow formulations and address some mass conservation issues from the previous work. We analyze these formulations and the nonlinear systems that result from applying the integration methods, placing particular emphasis on their index, range of applicability, and mass conservation characteristics. We conduct numerical experiments to study the behavior of the numerical models for three test problems. We demonstrate that higher order integration in time is more efficient than standard low-order methods for a variety of practical grids and integration tolerances, that the adaptive scheme successfully varies the step size in response to changing conditions, and that mass balance can be maintained efficiently using variable-order integration and an appropriately chosen numerical model formulation.

  20. Method and apparatus for detecting laminar flow separation and reattachment

    NASA Technical Reports Server (NTRS)

    Stack, John P. (Inventor); Mangalam, Sivaramakrishnan M. (Inventor)

    1989-01-01

    The invention is a method and apparatus for detecting laminar flow separation and flow reattachment of a fluid stream by simultaneously sensing and comparing a plurality of output signals, each representing the dynamic shear stress at one of an equal number of sensors spaced along a straight line on the surface of an airfoil or the like that extends parallel to the fluid stream. The output signals are concurrently compared to detect the sensors across which a reversal in phase of said output signal occurs, said detected sensors being in the region of laminar separation or reattachment. The novelty in this invention is the discovery and use of the phase reversal phenomena to detect laminar separation and attachment of a fluid stream from any surface such as an airfoil supported therein.

  1. A review of various flow-solid interaction analysis methods with emphasis on recent advances in turbulence models and flow analysis methods

    NASA Technical Reports Server (NTRS)

    Kim, S.-W.; Chen, C.-P.

    1988-01-01

    A review on various experimental, analytical, and numerical methods for the flow-solid interaction of a nest of cylinders subjected to cross flow is presented in this paper. In order to assess the possibility of initiating a development of major numerical analysis method for flow-solid interaction of a nest of cylinders in cross flows, a review on various turbulence models, numerical methods such as the finite element method and the finite difference method, and the available numerical methods for flow-solid interactions have been included in this paper. A multiple-time-scale turbulence model for complex turbulent flows and a finite element method for high Reynolds number flows have been introduced herein for the same reason.

  2. A nearly real-time UAV video flow mosaic method

    NASA Astrophysics Data System (ADS)

    Zheng, H.; Jiang, C.; Sun, M.; Li, X. D.; Xiang, R.; Liu, Lei

    2014-12-01

    In order to solve the problem of low accuracy and high computation cost of current video mosaic methods, and also to acquire large field of view images by the unmanned aerial vehicles (UAV), which have high accuracy and high resolution, this paper propose a method for near real-time mosaic of video flow, so that we can provide essential reference data for the earthquake relief, as well as post-disaster reconstruction and recovery, in time. In this method, we obtain the flight area scope in the route planning process, and calculate the sizes of each frame with sensor sizes and altitudes. Given an overlap degree, time intervals are calculated, and key frames are extracted. After that, feature points are detected in each frame, and they are matched using Hamming distance. The RANSAC algorithm is then applied to remove error matching and calculate parameters of the transformation model. In one-strip case, the newly extracted frame is taken as the reference image in the first half, while after the middle frame is extracted, it is the reference one until the end. Experimental results show that our method can reduce the cascading error, and improve the accuracy and quality of the mosaic images, near real-time mosaic of aerial video flow is feasible.

  3. An efficient pressure-correction method for incompressible multifluid flows

    NASA Astrophysics Data System (ADS)

    Dodd, M.; Ferrante, A.

    2013-11-01

    We present a new pressure-correction (PC) method for solving incompressible multifluid flows with large density ratios. The novelty of the method is that the variable coefficient Poisson equation that arises in solving the variable-density Navier-Stokes equations has been reduced to a constant coefficient equation, which can then be solved directly using a fast Poisson solver. The new method is coupled to our mass-conserving volume-of-fluid (VoF) method to capture the interface between the moving fluids. First, we verified the new PC/VoF solver using the capillary wave test-case up to density and viscosity ratios of 10,000. Then, we validated the new flow solver by simulating the motion of a falling water droplet in air by comparing the droplet terminal velocity with the experimental value (Beard, 1976) for 95 . 6 <= Re <= 473 , 0 . 06 <= We <= 0 . 61 , and 0 . 05 <= Bo <= 0 . 26 . We also verified the solver for a rising air bubble in water. The algorithm is shown to be second-order accurate, and stable for density and viscosity ratios up to 10,000. Also, we show that our fast Poisson solver is more than ten times faster than the Hypre multigrid solver up to a 10243 grid and 1024 cores. NSF CAREER #1054591.

  4. Incompressible material point method for free surface flow

    NASA Astrophysics Data System (ADS)

    Zhang, Fan; Zhang, Xiong; Sze, Kam Yim; Lian, Yanping; Liu, Yan

    2017-02-01

    To overcome the shortcomings of the weakly compressible material point method (WCMPM) for modeling the free surface flow problems, an incompressible material point method (iMPM) is proposed based on operator splitting technique which splits the solution of momentum equation into two steps. An intermediate velocity field is first obtained by solving the momentum equations ignoring the pressure gradient term, and then the intermediate velocity field is corrected by the pressure term to obtain a divergence-free velocity field. A level set function which represents the signed distance to free surface is used to track the free surface and apply the pressure boundary conditions. Moreover, an hourglass damping is introduced to suppress the spurious velocity modes which are caused by the discretization of the cell center velocity divergence from the grid vertexes velocities when solving pressure Poisson equations. Numerical examples including dam break, oscillation of a cubic liquid drop and a droplet impact into deep pool show that the proposed incompressible material point method is much more accurate and efficient than the weakly compressible material point method in solving free surface flow problems.

  5. Methods for blood flow measurements using ultrasound contrast agents

    NASA Astrophysics Data System (ADS)

    Fowlkes, J. Brian

    2003-10-01

    Blood flow measurements using ultrasound contrast agents are being investigated for myocardial perfusion and more recently in other organ systems. The methods are based largely on the relative increase in echogenicity due to the concentration of bubbles present in the ultrasound beam. In the simplest form, regional differences in blood volume can be inferred but the possibility exists to extract perfusion from the transit of contrast agent through tissue. Perfusion measurements rely on determining the flux of blood through a tissue volume and as such require knowledge of the fractional blood volume (FBV), i.e., ml blood/g tissue and the rate of exchange, commonly measured as the mean transit time (MTT). This presentation will discuss methods of determining each of these values and their combination to estimate tissue perfusion. Underlying principles of indicator-dilution theory will be provided in the context of ultrasound contrast agents. Current methods for determining MTT will include imaging of the intravenous bolus, in-plane contrast disruption with interval and real-time contrast recovery imaging, and control of contrast agent flow using arterial disruption (contrast interruption). The advantages and limitations of the methods will be examined along with current applications. [Work supported in part by NIH.

  6. A Level Set Method for vaporizing two-phase flows

    NASA Astrophysics Data System (ADS)

    Tanguy, Sébastien; Ménard, Thibaut; Berlemont, Alain

    2007-02-01

    Development and applications of numerical methods devoted to reactive interface simulations are presented. Emphasis is put on vaporization, where numerical difficulties arise in imposing accurate jump conditions for heat and mass transfers. We use both the Level Set Method and the Ghost Fluid Method to capture the interface motion accurately and to handle suitable jump conditions. A local vaporization mass flow rate per unit of surface area is defined and Stefan flow is involved in the process. Specific care has been devoted to the extension of discontinuous variables across the interface to populate ghost cells, in order to avoid parasitic currents and numerical diffusion across the interface. A projection method is set up to impose both the velocity field continuity and a divergence-free condition for the extended velocity field across the interface. The d2 law is verified in the numerical simulations of the vaporization of an isolated static drop. Results are then presented for a water droplet moving in air. Vapor mass fraction and temperature fields inside and outside the droplet are presented.

  7. A direct simulation method for flows with suspended paramagnetic particles

    SciTech Connect

    Kang, Tae Gon; Hulsen, Martien A. Toonder, Jaap M.J. den; Anderson, Patrick D.; Meijer, Han E.H.

    2008-04-20

    A direct numerical simulation method based on the Maxwell stress tensor and a fictitious domain method has been developed to solve flows with suspended paramagnetic particles. The numerical scheme enables us to take into account both hydrodynamic and magnetic interactions between particles in a fully coupled manner. Particles are assumed to be non-Brownian with negligible inertia. Rigid body motions of particles in 2D are described by a rigid-ring description implemented by Lagrange multipliers. The magnetic force, acting on the particles due to magnetic fields, is represented by the divergence of the Maxwell stress tensor, which acts as a body force added to the momentum balance equation. Focusing on two-dimensional problems, we solve a single-particle problem for verification. With the magnetic force working on the particle, the proper number of collocation points is found to be two points per element. The convergence with mesh refinement is verified by comparing results from regular mesh problems with those from a boundary-fitted mesh problem as references. We apply the developed method to two application problems: two-particle interaction in a uniform magnetic field and the motion of a magnetic chain in a rotating field, demonstrating the capability of the method to tackle general problems. In the motion of a magnetic chain, especially, the deformation pattern at break-up is similar to the experimentally observed one. The present formulation can be extended to three-dimensional and viscoelastic flow problems.

  8. A diffusive information preservation method for small Knudsen number flows

    SciTech Connect

    Fei, Fei; Fan, Jing

    2013-06-15

    The direct simulation Monte Carlo (DSMC) method is a powerful particle-based method for modeling gas flows. It works well for relatively large Knudsen (Kn) numbers, typically larger than 0.01, but quickly becomes computationally intensive as Kn decreases due to its time step and cell size limitations. An alternative approach was proposed to relax or remove these limitations, based on replacing pairwise collisions with a stochastic model corresponding to the Fokker–Planck equation [J. Comput. Phys., 229, 1077 (2010); J. Fluid Mech., 680, 574 (2011)]. Similar to the DSMC method, the downside of that approach suffers from computationally statistical noise. To solve the problem, a diffusion-based information preservation (D-IP) method has been developed. The main idea is to track the motion of a simulated molecule from the diffusive standpoint, and obtain the flow velocity and temperature through sampling and averaging the IP quantities. To validate the idea and the corresponding model, several benchmark problems with Kn ∼ 10{sup −3}–10{sup −4} have been investigated. It is shown that the IP calculations are not only accurate, but also efficient because they make possible using a time step and cell size over an order of magnitude larger than the mean collision time and mean free path, respectively.

  9. A diffusive information preservation method for small Knudsen number flows

    NASA Astrophysics Data System (ADS)

    Fei, Fei; Fan, Jing

    2013-06-01

    The direct simulation Monte Carlo (DSMC) method is a powerful particle-based method for modeling gas flows. It works well for relatively large Knudsen (Kn) numbers, typically larger than 0.01, but quickly becomes computationally intensive as Kn decreases due to its time step and cell size limitations. An alternative approach was proposed to relax or remove these limitations, based on replacing pairwise collisions with a stochastic model corresponding to the Fokker-Planck equation [J. Comput. Phys., 229, 1077 (2010); J. Fluid Mech., 680, 574 (2011)]. Similar to the DSMC method, the downside of that approach suffers from computationally statistical noise. To solve the problem, a diffusion-based information preservation (D-IP) method has been developed. The main idea is to track the motion of a simulated molecule from the diffusive standpoint, and obtain the flow velocity and temperature through sampling and averaging the IP quantities. To validate the idea and the corresponding model, several benchmark problems with Kn ˜ 10-3-10-4 have been investigated. It is shown that the IP calculations are not only accurate, but also efficient because they make possible using a time step and cell size over an order of magnitude larger than the mean collision time and mean free path, respectively.

  10. An optical flow-based method for velocity field of fluid flow estimation

    NASA Astrophysics Data System (ADS)

    Głomb, Grzegorz; Świrniak, Grzegorz; Mroczka, Janusz

    2017-06-01

    The aim of this paper is to present a method for estimating flow-velocity vector fields using the Lucas-Kanade algorithm. The optical flow measurements are based on the Particle Image Velocimetry (PIV) technique, which is commonly used in fluid mechanics laboratories in both research institutes and industry. Common approaches for an optical characterization of velocity fields base on computation of partial derivatives of the image intensity using finite differences. Nevertheless, the accuracy of velocity field computations is low due to the fact that an exact estimation of spatial derivatives is very difficult in presence of rapid intensity changes in the PIV images, caused by particles having small diameters. The method discussed in this paper solves this problem by interpolating the PIV images using Gaussian radial basis functions. This provides a significant improvement in the accuracy of the velocity estimation but, more importantly, allows for the evaluation of the derivatives in intermediate points between pixels. Numerical analysis proves that the method is able to estimate even a separate vector for each particle with a 5× 5 px2 window, whereas a classical correlation-based method needs at least 4 particle images. With the use of a specialized multi-step hybrid approach to data analysis the method improves the estimation of the particle displacement far above 1 px.

  11. An engineering aerodynamic heating method for hypersonic flow

    NASA Technical Reports Server (NTRS)

    Riley, Christopher J.; Dejarnette, Fred R.

    1992-01-01

    A capability to calculate surface heating rates has been incorporated in an approximate three-dimensional inviscid technique. Surface streamlines are calculated from the inviscid solution, and the axisymmetric analog is then used along with a set of approximate convective-heating equations to compute the surface heat transfer. The method is applied to blunted axisymmetric and three-dimensional ellipsoidal cones at angle of attack for the laminar flow of a perfect gas. The method is also applicable to turbulent and equilibrium-air conditions. The present technique predicts surface heating rates that compare favorably with experimental (ground-test and flight) data and numerical solutions of the Navier-Stokes (NS) and viscous shock-layer (VSL) equations. The new technique represents a significant improvement over current engineering aerothermal methods with only a modest increase in computational effort.

  12. An Engineering Aerodynamic Heating Method for Hypersonic Flow

    NASA Technical Reports Server (NTRS)

    Riley, Christopher J.; DeJarnette, Fred R.

    1992-01-01

    A capability to calculate surface heating rates has been incorporated in an approximate three-dimensional inviscid technique. Surface streamlines are calculated from the inviscid solution, and the axisymmetric analog is then used along with a set of approximate convective-heating equations to compute the surface heat transfer. The method is applied to blunted axisymmetric and three-dimensional ellipsoidal cones at angle of attack for the laminar flow of a perfect gas. The method is also applicable to turbulent and equilibrium-air conditions. The present technique predicts surface heating rates that compare favorably with experimental (ground-test and flight) data and numerical solutions of the Navier-Stokes (NS) and viscous shock-layer (VSL) equations. The new technique represents a significant improvement over current engineering aerothermal methods with only a modest increase in computational effort.

  13. Flow resistance and its prediction methods in compound channels

    NASA Astrophysics Data System (ADS)

    Yang, Kejun; Cao, Shuyou; Liu, Xingnian

    2007-02-01

    A series of experiments was carried out in a large symmetric compound channel composed of a rough main channel and rough floodplains to investigate the resistance characteristics of inbank and overbank flows. The effective Manning, Darcy-Weisbach, Chezy coefficients and the relative Nikuradse roughness height were analyzed. Many different representative methods for predicting the composite roughness were systematically summarized. Besides the measured data, a vast number of laboratory data and field data for compound channels were collected and used to check the validity of these methods for different subsection divisions including the vertical, horizontal, diagonal and bisectional divisions. The computation showed that these methods resulted in big errors in assessing the composite roughness in compound channels, and the reasons were analyzed in detail. The error magnitude is related to the subsection divisions.

  14. An engineering aerodynamic heating method for hypersonic flow

    NASA Technical Reports Server (NTRS)

    Riley, Christopher J.; Dejarnette, Fred R.

    1992-01-01

    A capability to calculate surface heating rates has been incorporated in an approximate three-dimensional inviscid technique. Surface streamlines are calculated from the inviscid solution, and the axisymmetric analog is then used along with a set of approximate convective-heating equations to compute the surface heat transfer. The method is applied to blunted axisymmetric and three-dimensional ellipsoidal cones at angle of attack for the laminar flow of a perfect gas. The method is also applicable to turbulent and equilibrium-air conditions. The present technique predicts surface heating rates that compare favorably with experimental (ground-test and flight) data and numerical solutions of the Navier-Stokes (NS) and viscous shock-layer (VSL) equations. The new technique represents a significant improvement over current engineering aerothermal methods with only a modest increase in computational effort.

  15. Biochemical oxygen demand measurement by mediator method in flow system.

    PubMed

    Liu, Ling; Bai, Lu; Yu, Dengbin; Zhai, Junfeng; Dong, Shaojun

    2015-06-01

    Using mediator as electron acceptor for biochemical oxygen demand (BOD) measurement was developed in the last decade (BODMed). However, until now, no BOD(Med) in a flow system has been reported. This work for the first time describes a flow system of BOD(Med) method (BOD(Med)-FS) by using potassium ferricyanide as mediator and carbon fiber felt as substrate material for microbial immobilization. The system can determine the BOD value within 30 min and possesses a wider analytical linear range for measuring glucose-glutamic acid (GGA) standard solution from 2 up to 200 mg L(-1) without the need of dilution. The analytical performance of the BOD(Med)-FS is comparable or better than that of the previously reported BOD(Med) method, especially its superior long-term stability up to 2 months under continuous operation. Moreover, the BOD(Med)-FS has same determination accuracy with the conventional BOD5 method by measuring real samples from a local wastewater treatment plant (WWTP). Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Vortex methods for two- and three-dimensional flow simulations

    NASA Technical Reports Server (NTRS)

    Leonard, A.

    1980-01-01

    The point vortex and vortex blob methods for two dimensional flows are presented. Several results are discussed concerning the numerical analysis of the latter scheme, e.g., the preservation of globally conserved quantities and the analysis of the spatial discretization error resulting from the convection of fixed blobs of vorticity. An application to the two dimensional mixing layer is briefly described. The contour dynamics method is also discussed. The simulation of three dimensional flows with vortex methods is discussed. A natural way to represent the vorticity is in the form of closed tubes of filaments of vorticity, although other schemes are examined. Applications to aircraft trailing vortices and to a turbulent spot in a laminar boundary layer are presented. Hybrid schemes that use an Eulerian mesh to solve the Poisson equation for the velocity field are discussed. The goal of these schemes is to avoid the high cost of the Biot-Savart integration if many vortex elements are used while enjoying most of the advantages of pure Lagrangian schemes.

  17. Massively parallel simulations of multiphase flows using Lattice Boltzmann methods

    NASA Astrophysics Data System (ADS)

    Ahrenholz, Benjamin

    2010-03-01

    In the last two decades the lattice Boltzmann method (LBM) has matured as an alternative and efficient numerical scheme for the simulation of fluid flows and transport problems. Unlike conventional numerical schemes based on discretizations of macroscopic continuum equations, the LBM is based on microscopic models and mesoscopic kinetic equations. The fundamental idea of the LBM is to construct simplified kinetic models that incorporate the essential physics of microscopic or mesoscopic processes so that the macroscopic averaged properties obey the desired macroscopic equations. Especially applications involving interfacial dynamics, complex and/or changing boundaries and complicated constitutive relationships which can be derived from a microscopic picture are suitable for the LBM. In this talk a modified and optimized version of a Gunstensen color model is presented to describe the dynamics of the fluid/fluid interface where the flow field is based on a multi-relaxation-time model. Based on that modeling approach validation studies of contact line motion are shown. Due to the fact that the LB method generally needs only nearest neighbor information, the algorithm is an ideal candidate for parallelization. Hence, it is possible to perform efficient simulations in complex geometries at a large scale by massively parallel computations. Here, the results of drainage and imbibition (Degree of Freedom > 2E11) in natural porous media gained from microtomography methods are presented. Those fully resolved pore scale simulations are essential for a better understanding of the physical processes in porous media and therefore important for the determination of constitutive relationships.

  18. Polymeric drug nanoparticles prepared by an aerosol flow reactor method.

    PubMed

    Eerikäinen, Hannele; Kauppinen, Esko I; Kansikas, Jarno

    2004-01-01

    Our purpose was to study the possibility of using a novel method, namely, aerosol flow reactor method, for the preparation of drug-containing nanoparticles with varying amounts of drug and polymer. The physical properties of the prepared nanoparticles were analyzed. The nanoparticle size distributions were measured using differential mobility analyzer. The structure of the prepared nanoparticles was assessed by x-ray diffraction, differential scanning calorimetry, and electron microscopy. Drug release from the nanoparticles was analyzed. The spherical particles produced showed a unimodal and lognormal size distribution, and the geometric number mean size of the nanoparticles could be varied between 90 and 200 nm. When the amount of drug in the polymeric matrix was small, the nanoparticles had a homogeneous, amorphous structure. Drug crystals were formed when the amount of drug was increased over the solubility limit of the drug into the polymer. The amounts of drug and polymer controlled the drug release from the nanoparticles. The aerosol flow reactor method was found to be able to produce homogeneous amorphous matrix-type nanoparticles that can directly be collected as dry powder.

  19. COMPREHENSIVE METHOD OF CHARACTERISTICS MODELS FOR FLOW SIMULATION.

    USGS Publications Warehouse

    Lai, Chintu

    1988-01-01

    The use of the specified time interval (STI) numerical schemes has been popular in applying the method of characteristics (MOC) to unsteady open-channel flow problems. Studies and analyses of several variants of the STI schemes have led to the derivation of a new scheme, referred to herein as the multimode scheme, which combines implicit, temporal reachback, spatial reachback, and classical schemes into one. Three numerical models have been developed to implement the implicit and multimode schemes. Numerical analyses, numerical experiments, and field applications that verify, support, and demonstrate the enhanced model capabilities are presented.

  20. A boundary element method for steady incompressible thermoviscous flow

    NASA Technical Reports Server (NTRS)

    Dargush, G. F.; Banerjee, P. K.

    1991-01-01

    A boundary element formulation is presented for moderate Reynolds number, steady, incompressible, thermoviscous flows. The governing integral equations are written exclusively in terms of velocities and temperatures, thus eliminating the need for the computation of any gradients. Furthermore, with the introduction of reference velocities and temperatures, volume modeling can often be confined to only a small portion of the problem domain, typically near obstacles or walls. The numerical implementation includes higher order elements, adaptive integration and multiregion capability. Both the integral formulation and implementation are discussed in detail. Several examples illustrate the high level of accuracy that is obtainable with the current method.

  1. Testing the global flow reconstruction method on coupled chaotic oscillators

    NASA Astrophysics Data System (ADS)

    Plachy, Emese; Kolláth, Zoltán

    2010-03-01

    Irregular behaviour of pulsating variable stars may occur due to low dimensional chaos. To determine the quantitative properties of the dynamics in such systems, we apply a suitable time series analysis, the global flow reconstruction method. The robustness of the reconstruction can be tested through the resultant quantities, like Lyapunov dimension and Fourier frequencies. The latter is specially important as it is directly derivable from the observed light curves. We have performed tests using coupled Rossler oscillators to investigate the possible connection between those quantities. In this paper we present our test results.

  2. A Flow SPR Immunosensor Based on a Sandwich Direct Method

    PubMed Central

    Tomassetti, Mauro; Conta, Giorgia; Campanella, Luigi; Favero, Gabriele; Sanzò, Gabriella; Mazzei, Franco; Antiochia, Riccarda

    2016-01-01

    In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10−3 and 10−1 M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes. PMID:27187486

  3. Simple numerical method for predicting steady compressible flows

    NASA Technical Reports Server (NTRS)

    Von Lavante, E.; Melson, N. Duane

    1987-01-01

    The present numerical method for the solution of the isenthalpic form of the governing equations for compressible viscous and inviscid flows has its basis in the concept of flux vector splitting in its implicit form, and has been tested in the cases of several difficult viscous and inviscid configurations. An acceleration of time-marching to steady state is accomplished by implementing a multigrid procedure which effectively increases the convergence rate. The steady state results obtained are largely of good quality, and required only short computational times.

  4. An analysis method for two-dimensional transonic viscous flow

    NASA Technical Reports Server (NTRS)

    Bavitz, P. C.

    1975-01-01

    A method for the approximate calculation of transonic flow over airfoils, including shock waves and viscous effects, is described. Numerical solutions are obtained by use of a computer program which is discussed in the appendix. The importance of including the boundary layer in the analysis is clearly demonstrated, as well as the need to improve on existing procedures near the trailing edge. Comparisons between calculations and experimental data are presented for both conventional and supercritical airfoils, emphasis being on the surface pressure distribution, and good agreement is indicated.

  5. Method for flow cytometric detection of Listeria monocytogenes in milk.

    PubMed Central

    Donnelly, C W; Baigent, G J

    1986-01-01

    This report describes a method for the detection of Listeria monocytogenes in raw milk by flow cytometric analysis of fluorescently labeled bacterial populations. The use of immunofluorescence in combination with measures of DNA content by propidium iodide labeling and size by light scattering enabled specific identification of L. monocytogenes from Streptococcus faecalis, Streptococcus agalactiae, Streptococcus uberis, Staphylococcus epidermidis, and Staphylococcus hyicus. Additional specific resolution of L. monocytogenes populations was achieved through selective enrichment of raw milk in Listeria enrichment broth. These procedures should permit the rapid screening of milk and other food samples for L. monocytogenes and eliminate many of the short-comings associated with conventional fluorescent-antibody procedures. PMID:3096202

  6. Parabolized Navier-Stokes methods for hypersonic flows

    NASA Technical Reports Server (NTRS)

    Lawrence, Scott L.

    1991-01-01

    A representative sampling of the techniques used in the integration of the Parabolized Navier-Stokes (PNS) equations is presented. Special atention is given to recent algorithms developed specifically for application to high speed flows, characterized by the presence of strong embedded shock waves and real gas effects. It is shown that PNS solvers are being used in the analysis of sonic boom signatures. Methods for modeling physical effects are discussed, including an overview of commonly used turbulence models and a more detailed discussion of techniques for including equilibrium and finite rate real gas effects.

  7. INSTRUMENTS AND METHODS OF INVESTIGATION: Invisible cloaking of material bodies using the wave flow method

    NASA Astrophysics Data System (ADS)

    Dubinov, Alexander E.; Mytareva, Lyubov'A.

    2010-08-01

    The current knowledge of the physics of electromagnetic cloaking of material objects by the wave flow method is reviewed. Experiments demonstrating the feasibility of this cloaking method are described. Some aspects of calculating cloak profiles are examined, and achievements and unsolved problems in the theory of the interaction of electromagnetic waves with shells are considered. Prospects for developing the cloaking method for waves of other physical nature (acoustic and probability density waves) are discussed.

  8. Fast multipole method applied to Lagrangian simulations of vortical flows

    NASA Astrophysics Data System (ADS)

    Ricciardi, Túlio R.; Wolf, William R.; Bimbato, Alex M.

    2017-10-01

    Lagrangian simulations of unsteady vortical flows are accelerated by the multi-level fast multipole method, FMM. The combination of the FMM algorithm with a discrete vortex method, DVM, is discussed for free domain and periodic problems with focus on implementation details to reduce numerical dissipation and avoid spurious solutions in unsteady inviscid flows. An assessment of the FMM-DVM accuracy is presented through a comparison with the direct calculation of the Biot-Savart law for the simulation of the temporal evolution of an aircraft wake in the Trefftz plane. The role of several parameters such as time step restriction, truncation of the FMM series expansion, number of particles in the wake discretization and machine precision is investigated and we show how to avoid spurious instabilities. The FMM-DVM is also applied to compute the evolution of a temporal shear layer with periodic boundary conditions. A novel approach is proposed to achieve accurate solutions in the periodic FMM. This approach avoids a spurious precession of the periodic shear layer and solutions are shown to converge to the direct Biot-Savart calculation using a cotangent function.

  9. Multi-scale simulation method for electroosmotic flows

    NASA Astrophysics Data System (ADS)

    Guo, Lin; Chen, Shiyi; Robbins, Mark O.

    2016-10-01

    Electroosmotic transport in micro-and nano- channels has important applications in biological and engineering systems but is difficult to model because nanoscale structure near surfaces impacts flow throughout the channel. We develop an efficient multi-scale simulation method that treats near-wall and bulk subdomains with different physical descriptions and couples them through a finite overlap region. Molecular dynamics is used in the near-wall subdomain where the ion density is inconsistent with continuum models and the discrete structure of solvent molecules is important. In the bulk region the solvent is treated as a continuum fluid described by the incompressible Navier-Stokes equations with thermal fluctuations. A discrete description of ions is retained because of the low density of ions and the long range of electrostatic interactions. A stochastic Euler-Lagrangian method is used to simulate the dynamics of these ions in the implicit continuum solvent. The overlap region allows free exchange of solvent and ions between the two subdomains. The hybrid approach is validated against full molecular dynamics simulations for different geometries and types of flows.

  10. An Anelastic Allspeed Projection Method for GravitationallyStratified Flows

    SciTech Connect

    Gatti-Bono, Caroline; Colella, Phillip

    2005-02-24

    This paper looks at gravitationally-stratified atmospheric flows at low Mach and Froude numbers and proposes a new algorithm to solve the compressible Euler equations, in which the asymptotic limits are recovered numerically and the boundary conditions for block-structured local refinement methods are well-posed. The model is non-hydrostatic and the numerical algorithm uses a splitting to separate the fast acoustic dynamics from the slower anelastic dynamics. The acoustic waves are treated implicitly while the anelastic dynamics is treated semi-implicitly and an embedded-boundary method is used to represent mountain ranges. We present an example that verifies our asymptotic analysis and a set of results that compares very well with the classical gravity wave results presented by Durran.

  11. Traffic flow equations coming from the Grad's method.

    NASA Astrophysics Data System (ADS)

    Velasco, Rosa M.; Méndez, Alma R.

    2006-11-01

    The usual Grad's method in kinetic theory of gases is developed to construct a new model in traffic flow problems. This is applied to the kinetic equation called as the Paveri-Fontana equation which tells us how the distribution function evolves in time [1]. We assume a special model for the desired velocity of drivers [2] and the Grad's method provides us with a closure relation in the macroscopic equations. The simulation results for this model allow us to find the behavior of density, mean velocity and the velocity variance in the system. All the results are consistent with the validity region of the kinetic equation and with the qualitative behavior proper to traffic models. We show some comparisons with other models in the literature [3]. [1] S.L Paveri-Fontana; Transp. Res. 9 (1975), 225. [2] R.M. Velasco, W. Marques Jr.; Phys. Rev. E72 (2005), 046102. [3] D. Helbing; Phys. Rev. E51 (1995), 3164.

  12. Preconditioning Newton-Krylor Methods for Variably Saturated Flow

    SciTech Connect

    Woodward, C.; Jones, J

    2000-01-07

    In this paper, we compare the effectiveness of three preconditioning strategies in simulations of variably saturated flow. Using Richards' equation as our model, we solve the nonlinear system using a Newton-Krylov method. Since Krylov solvers can stagnate, resulting in slow convergence, we investigate different strategies of preconditioning the Jacobian system. Our work uses a multigrid method to solve the preconditioning systems, with three different approximations to the Jacobian matrix. One approximation lags the nonlinearities, the second results from discarding selected off-diagonal contributions, and the third matrix considered is the full Jacobian. Results indicate that although the Jacobian is more accurate, its usage as a preconditioning matrix should be limited, as it requires much more storage than the simpler approximations. Also, simply lagging the nonlinearities gives a preconditioning matrix that is almost as effective as the full Jacobian but much easier to compute.

  13. Finite area method for nonlinear supersonic conical flows

    NASA Technical Reports Server (NTRS)

    Sritharan, S. S.; Seebass, A. R.

    1983-01-01

    A fully conservative numerical method for the computation of steady inviscid supersonic flow about general conical bodies at incidence is described. The procedure utilizes the potential approximation and implements a body conforming mesh generator. The conical potential is assumed to have its best linear variation inside each mesh cell; a secondary interlocking cell system is used to establish the flux balance required to conserve mass. In the supersonic regions the scheme is symmetrized by adding artificial viscosity in conservation form. The algorithm is nearly an order of a magnitude faster than present Euler methods and predicts known results accurately and qualitative features such as nodal point lift off correctly. Results are compared with those of other investigators.

  14. Method and apparatus for controlling the flow rate of mercury in a flow system

    DOEpatents

    Grossman, Mark W.; Speer, Richard

    1991-01-01

    A method for increasing the mercury flow rate to a photochemical mercury enrichment utilizing an entrainment system comprises the steps of passing a carrier gas over a pool of mercury maintained at a first temperature T1, wherein the carrier gas entrains mercury vapor; passing said mercury vapor entrained carrier gas to a second temperature zone T2 having temperature less than T1 to condense said entrained mercury vapor, thereby producing a saturated Hg condition in the carrier gas; and passing said saturated Hg carrier gas to said photochemical enrichment reactor.

  15. Approximate hard-sphere method for densely packed granular flows

    NASA Astrophysics Data System (ADS)

    Guttenberg, Nicholas

    2011-05-01

    The simulation of granular media is usually done either with event-driven codes that treat collisions as instantaneous but have difficulty with very dense packings, or with molecular dynamics (MD) methods that approximate rigid grains using a stiff viscoelastic spring. There is a little-known method that combines several collision events into a single timestep to retain the instantaneous collisions of event-driven dynamics, but also be able to handle dense packings. However, it is poorly characterized as to its regime of validity and failure modes. We present a modification of this method to reduce the introduction of overlap error, and test it using the problem of two-dimensional (2D) granular Couette flow, a densely packed system that has been well characterized by previous work. We find that this method can successfully replicate the results of previous work up to the point of jamming, and that it can do so a factor of 10 faster than comparable MD methods.

  16. Approximate hard-sphere method for densely packed granular flows.

    PubMed

    Guttenberg, Nicholas

    2011-05-01

    The simulation of granular media is usually done either with event-driven codes that treat collisions as instantaneous but have difficulty with very dense packings, or with molecular dynamics (MD) methods that approximate rigid grains using a stiff viscoelastic spring. There is a little-known method that combines several collision events into a single timestep to retain the instantaneous collisions of event-driven dynamics, but also be able to handle dense packings. However, it is poorly characterized as to its regime of validity and failure modes. We present a modification of this method to reduce the introduction of overlap error, and test it using the problem of two-dimensional (2D) granular Couette flow, a densely packed system that has been well characterized by previous work. We find that this method can successfully replicate the results of previous work up to the point of jamming, and that it can do so a factor of 10 faster than comparable MD methods.

  17. Discontinuous Galerkin method for multicomponent chemically reacting flows and combustion

    NASA Astrophysics Data System (ADS)

    Lv, Yu; Ihme, Matthias

    2014-08-01

    This paper presents the development of a discontinuous Galerkin (DG) method for application to chemically reacting flows in subsonic and supersonic regimes under the consideration of variable thermo-viscous-diffusive transport properties, detailed and stiff reaction chemistry, and shock capturing. A hybrid-flux formulation is developed for treatment of the convective fluxes, combining a conservative Riemann-solver and an extended double-flux scheme. A computationally efficient splitting scheme is proposed, in which advection and diffusion operators are solved in the weak form, and the chemically stiff substep is advanced in the strong form using a time-implicit scheme. The discretization of the viscous-diffusive transport terms follows the second form of Bassi and Rebay, and the WENO-based limiter due to Zhong and Shu is extended to multicomponent systems. Boundary conditions are developed for subsonic and supersonic flow conditions, and the algorithm is coupled to thermochemical libraries to account for detailed reaction chemistry and complex transport. The resulting DG method is applied to a series of test cases of increasing physico-chemical complexity. Beginning with one- and two-dimensional multispecies advection and shock-fluid interaction problems, computational efficiency, convergence, and conservation properties are demonstrated. This study is followed by considering a series of detonation and supersonic combustion problems to investigate the convergence-rate and the shock-capturing capability in the presence of one- and multistep reaction chemistry. The DG algorithm is then applied to diffusion-controlled deflagration problems. By examining convergence properties for polynomial order and spatial resolution, and comparing these with second-order finite-volume solutions, it is shown that optimal convergence is achieved and that polynomial refinement provides advantages in better resolving the localized flame structure and complex flow-field features

  18. In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry

    PubMed Central

    Lelliott, Patrick M.; McMorran, Brendan J.; Foote, Simon J.; Burgio, Gaetan

    2015-01-01

    During blood stage infection, malaria parasites invade, mature, and replicate within red blood cells (RBCs). This results in a regular growth cycle and an exponential increase in the proportion of malaria infected RBCs, known as parasitemia. We describe a flow cytometry based protocol which utilizes a combination of the DNA dye Hoechst, and the mitochondrial membrane potential dye, JC-1, to identify RBCs which contain parasites and therefore the parasitemia, of in vivo blood samples from Plasmodium chabaudi adami DS infected mice. Using this approach, in combination with fluorescently conjugated antibodies, parasitized RBCs can be distinguished from leukocytes, RBC progenitors, and RBCs containing Howell-Jolly bodies (HJ-RBCs), with a limit of detection of 0.007% parasitemia. Additionally, we outline a method for the comparative assessment of merozoite invasion into two different RBC populations. In this assay RBCs, labeled with two distinct compounds identifiable by flow cytometry, are transfused into infected mice. The relative rate of invasion into the two populations can then be assessed by flow cytometry based on the proportion of parasitized RBCs in each population over time. This combined approach allows the accurate measurement of both parasitemia and merozoite invasion in an in vivo model of malaria infection. PMID:25867202

  19. A new general 1-D vadose zone flow solution method

    NASA Astrophysics Data System (ADS)

    Ogden, Fred L.; Lai, Wencong; Steinke, Robert C.; Zhu, Jianting; Talbot, Cary A.; Wilson, John L.

    2015-06-01

    We have developed an alternative to the one-dimensional partial differential equation (PDE) attributed to Richards (1931) that describes unsaturated porous media flow in homogeneous soil layers. Our solution is a set of three ordinary differential equations (ODEs) derived from unsaturated flux and mass conservation principles. We used a hodograph transformation, the Method of Lines, and a finite water-content discretization to produce ODEs that accurately simulate infiltration, falling slugs, and groundwater table dynamic effects on vadose zone fluxes. This formulation, which we refer to as "finite water-content", simulates sharp fronts and is guaranteed to conserve mass using a finite-volume solution. Our ODE solution method is explicitly integrable, does not require iterations and therefore has no convergence limits and is computationally efficient. The method accepts boundary fluxes including arbitrary precipitation, bare soil evaporation, and evapotranspiration. The method can simulate heterogeneous soils using layers. Results are presented in terms of fluxes and water content profiles. Comparing our method against analytical solutions, laboratory data, and the Hydrus-1D solver, we find that predictive performance of our finite water-content ODE method is comparable to or in some cases exceeds that of the solution of Richards' equation, with or without a shallow water table. The presented ODE method is transformative in that it offers accuracy comparable to the Richards (1931) PDE numerical solution, without the numerical complexity, in a form that is robust, continuous, and suitable for use in large watershed and land-atmosphere simulation models, including regional-scale models of coupled climate and hydrology.

  20. Bluff Body Flow Simulation Using a Vortex Element Method

    SciTech Connect

    Anthony Leonard; Phillippe Chatelain; Michael Rebel

    2004-09-30

    Heavy ground vehicles, especially those involved in long-haul freight transportation, consume a significant part of our nation's energy supply. it is therefore of utmost importance to improve their efficiency, both to reduce emissions and to decrease reliance on imported oil. At highway speeds, more than half of the power consumed by a typical semi truck goes into overcoming aerodynamic drag, a fraction which increases with speed and crosswind. Thanks to better tools and increased awareness, recent years have seen substantial aerodynamic improvements by the truck industry, such as tractor/trailer height matching, radiator area reduction, and swept fairings. However, there remains substantial room for improvement as understanding of turbulent fluid dynamics grows. The group's research effort focused on vortex particle methods, a novel approach for computational fluid dynamics (CFD). Where common CFD methods solve or model the Navier-Stokes equations on a grid which stretches from the truck surface outward, vortex particle methods solve the vorticity equation on a Lagrangian basis of smooth particles and do not require a grid. They worked to advance the state of the art in vortex particle methods, improving their ability to handle the complicated, high Reynolds number flow around heavy vehicles. Specific challenges that they have addressed include finding strategies to accurate capture vorticity generation and resultant forces at the truck wall, handling the aerodynamics of spinning bodies such as tires, application of the method to the GTS model, computation time reduction through improved integration methods, a closest point transform for particle method in complex geometrics, and work on large eddy simulation (LES) turbulence modeling.

  1. Numerical method of characteristics for one-dimensional blood flow

    NASA Astrophysics Data System (ADS)

    Acosta, Sebastian; Puelz, Charles; Rivière, Béatrice; Penny, Daniel J.; Rusin, Craig G.

    2015-08-01

    Mathematical modeling at the level of the full cardiovascular system requires the numerical approximation of solutions to a one-dimensional nonlinear hyperbolic system describing flow in a single vessel. This model is often simulated by computationally intensive methods like finite elements and discontinuous Galerkin, while some recent applications require more efficient approaches (e.g. for real-time clinical decision support, phenomena occurring over multiple cardiac cycles, iterative solutions to optimization/inverse problems, and uncertainty quantification). Further, the high speed of pressure waves in blood vessels greatly restricts the time step needed for stability in explicit schemes. We address both cost and stability by presenting an efficient and unconditionally stable method for approximating solutions to diagonal nonlinear hyperbolic systems. Theoretical analysis of the algorithm is given along with a comparison of our method to a discontinuous Galerkin implementation. Lastly, we demonstrate the utility of the proposed method by implementing it on small and large arterial networks of vessels whose elastic and geometrical parameters are physiologically relevant.

  2. Domain decomposition methods for the parallel computation of reacting flows

    NASA Technical Reports Server (NTRS)

    Keyes, David E.

    1988-01-01

    Domain decomposition is a natural route to parallel computing for partial differential equation solvers. Subdomains of which the original domain of definition is comprised are assigned to independent processors at the price of periodic coordination between processors to compute global parameters and maintain the requisite degree of continuity of the solution at the subdomain interfaces. In the domain-decomposed solution of steady multidimensional systems of PDEs by finite difference methods using a pseudo-transient version of Newton iteration, the only portion of the computation which generally stands in the way of efficient parallelization is the solution of the large, sparse linear systems arising at each Newton step. For some Jacobian matrices drawn from an actual two-dimensional reacting flow problem, comparisons are made between relaxation-based linear solvers and also preconditioned iterative methods of Conjugate Gradient and Chebyshev type, focusing attention on both iteration count and global inner product count. The generalized minimum residual method with block-ILU preconditioning is judged the best serial method among those considered, and parallel numerical experiments on the Encore Multimax demonstrate for it approximately 10-fold speedup on 16 processors.

  3. Numerical Method of Characteristics for One-Dimensional Blood Flow.

    PubMed

    Acosta, Sebastian; Puelz, Charles; Riviére, Béatrice; Penny, Daniel J; Rusin, Craig G

    2015-08-01

    Mathematical modeling at the level of the full cardiovascular system requires the numerical approximation of solutions to a one-dimensional nonlinear hyperbolic system describing flow in a single vessel. This model is often simulated by computationally intensive methods like finite elements and discontinuous Galerkin, while some recent applications require more efficient approaches (e.g. for real-time clinical decision support, phenomena occurring over multiple cardiac cycles, iterative solutions to optimization/inverse problems, and uncertainty quantification). Further, the high speed of pressure waves in blood vessels greatly restricts the time step needed for stability in explicit schemes. We address both cost and stability by presenting an efficient and unconditionally stable method for approximating solutions to diagonal nonlinear hyperbolic systems. Theoretical analysis of the algorithm is given along with a comparison of our method to a discontinuous Galerkin implementation. Lastly, we demonstrate the utility of the proposed method by implementing it on small and large arterial networks of vessels whose elastic and geometrical parameters are physiologically relevant.

  4. Numerical Method of Characteristics for One–Dimensional Blood Flow

    PubMed Central

    Puelz, Charles; Riviére, Béatrice; Penny, Daniel J.; Rusin, Craig G.

    2015-01-01

    Mathematical modeling at the level of the full cardiovascular system requires the numerical approximation of solutions to a one-dimensional nonlinear hyperbolic system describing flow in a single vessel. This model is often simulated by computationally intensive methods like finite elements and discontinuous Galerkin, while some recent applications require more efficient approaches (e.g. for real-time clinical decision support, phenomena occurring over multiple cardiac cycles, iterative solutions to optimization/inverse problems, and uncertainty quantification). Further, the high speed of pressure waves in blood vessels greatly restricts the time step needed for stability in explicit schemes. We address both cost and stability by presenting an efficient and unconditionally stable method for approximating solutions to diagonal nonlinear hyperbolic systems. Theoretical analysis of the algorithm is given along with a comparison of our method to a discontinuous Galerkin implementation. Lastly, we demonstrate the utility of the proposed method by implementing it on small and large arterial networks of vessels whose elastic and geometrical parameters are physiologically relevant. PMID:25931614

  5. Tensor product decomposition methods applied to complex flow data

    NASA Astrophysics Data System (ADS)

    von Larcher, Thomas; Klein, Rupert; Schneider, Reinhold; Wolf, Sebastian; Huber, Benjamin

    2017-04-01

    Low-rank multilevel approximation methods are an important tool in numerical analysis and in scientific computing. Those methods are often suited to attack high-dimensional problems successfully and allow very compact representations of large data sets. Specifically, hierarchical tensor product decomposition methods emerge as an promising approach for application to data that are concerned with cascade-of-scales problems as, e.g., in turbulent fluid dynamics. We focus on two particular objectives, that is representing turbulent data in an appropriate compact form and, secondly and as a long-term goal, finding self-similar vortex structures in multiscale problems. The question here is whether tensor product methods can support the development of improved understanding of the multiscale behavior and whether they are an improved starting point in the development of compact storage schemes for solutions of such problems relative to linear ansatz spaces. We present the reconstruction capabilities of a tensor decomposition based modeling approach tested against 3D turbulent channel flow data.

  6. Flow Diode and Method for Controlling Fluid Flow Origin of the Invention

    NASA Technical Reports Server (NTRS)

    Dyson, Rodger W (Inventor)

    2015-01-01

    A flow diode configured to permit fluid flow in a first direction while preventing fluid flow in a second direction opposite the first direction is disclosed. The flow diode prevents fluid flow without use of mechanical closures or moving parts. The flow diode utilizes a bypass flowline whereby all fluid flow in the second direction moves into the bypass flowline having a plurality of tortuous portions providing high fluidic resistance. The portions decrease in diameter such that debris in the fluid is trapped. As fluid only travels in one direction through the portions, the debris remains trapped in the portions.

  7. Finite volume methods for submarine debris flows and generated waves

    NASA Astrophysics Data System (ADS)

    Kim, Jihwan; Løvholt, Finn; Issler, Dieter

    2016-04-01

    Submarine landslides can impose great danger to the underwater structures and generate destructive tsunamis. Submarine debris flows often behave like visco-plastic materials, and the Herschel-Bulkley rheological model is known to be appropriate for describing the motion. In this work, we develop numerical schemes for the visco-plastic debris flows using finite volume methods in Eulerian coordinates with two horizontal dimensions. We provide parameter sensitivity analysis and demonstrate how common ad-hoc assumptions such as including a minimum shear layer depth influence the modeling of the landslide dynamics. Hydrodynamic resistance forces, hydroplaning, and remolding are all crucial terms for underwater landslides, and are hence added into the numerical formulation. The landslide deformation is coupled to the water column and simulated in the Clawpack framework. For the propagation of the tsunamis, the shallow water equations and the Boussinesq-type equations are employed to observe how important the wave dispersion is. Finally, two cases in central Norway, i.e. the subaerial quick clay landslide at Byneset in 2012, and the submerged tsunamigenic Statland landslide in 2014, are both presented for validation. The research leading to these results has received funding from the Research Council of Norway under grant number 231252 (Project TsunamiLand) and the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement 603839 (Project ASTARTE).

  8. On subsonic compressible flows by a method of correspondence II : application of methods to studies of flow with circulation about a circular cylinder

    NASA Technical Reports Server (NTRS)

    GELBART ABE; Bartnoff, Shepard

    1947-01-01

    A general method for studying the flow of a compressible fluid around a closed body was discussed in Part I of this report. Here, application is made to the specific case in which the linearized equation of state is used. For a given incompressible flow around a specific profile, a corresponding compressible flow is found. The flow at infinity remains unchanged. Detailed studies are made of the flow with circulation around a unit circle, and velocity distributions are found for a wide range of Mach number and angle of attack. Comparisons are made with other methods.

  9. Design of a Variational Multiscale Method for Turbulent Compressible Flows

    NASA Technical Reports Server (NTRS)

    Diosady, Laslo Tibor; Murman, Scott M.

    2013-01-01

    A spectral-element framework is presented for the simulation of subsonic compressible high-Reynolds-number flows. The focus of the work is maximizing the efficiency of the computational schemes to enable unsteady simulations with a large number of spatial and temporal degrees of freedom. A collocation scheme is combined with optimized computational kernels to provide a residual evaluation with computational cost independent of order of accuracy up to 16th order. The optimized residual routines are used to develop a low-memory implicit scheme based on a matrix-free Newton-Krylov method. A preconditioner based on the finite-difference diagonalized ADI scheme is developed which maintains the low memory of the matrix-free implicit solver, while providing improved convergence properties. Emphasis on low memory usage throughout the solver development is leveraged to implement a coupled space-time DG solver which may offer further efficiency gains through adaptivity in both space and time.

  10. The Augmented Lagrangian Method Applied to Unsolvable Power Flows

    NASA Astrophysics Data System (ADS)

    Zambaldi, Mario C.; Francisco, Juliano B.; Barboza, Luciano V.

    2011-11-01

    This work aims to present and discuss an approach to restore the network electric equations solvability. The unsolvable power flow is modeled as a constrained optimization problem. The cost function is the least squares of the real and reactive power mismatches sum. The equality constraints are the real and reactive power mismatches at null injection buses and/or at those buses that must have their power demands totally supplied for technical or economical criteria. The mathematical model is solved by an algorithm based on the Augmented Lagrangian method considering the particular structure of the problem. Numerical results for a real equivalent system from the Brazilian South-Southeast region are presented in order to assess the performance of the proposed approach.

  11. Valuing instream flows using the hedonic price method

    NASA Astrophysics Data System (ADS)

    Netusil, Noelwah R.; Summers, Matthew T.

    2009-11-01

    The Oregon Water Trust (OWT) uses a market-based approach to protect and enhance instream flows in Oregon. We use the hedonic price method to estimate the effect of numerous variables on the annualized price OWT pays for water rights: the amount of water protected by the transaction, transaction type (state approved or contractual agreement), presence of anadromous and/or resident fish, and if a fish is listed under the Endangered Species Act (ESA). We find evidence of a premium for state-approved transactions and for transactions that protect water in streams with listed species. Adjusting the amount of water protected by each transaction to include only rights that will be delivered with a high degree of certainty produces coefficient estimates that are similar, but more accurate, than using unadjusted water rights amounts.

  12. Methods for estimating flow-duration and annual mean-flow statistics for ungaged streams in Oklahoma

    USGS Publications Warehouse

    Esralew, Rachel A.; Smith, S. Jerrod

    2010-01-01

    Flow statistics can be used to provide decision makers with surface-water information needed for activities such as water-supply permitting, flow regulation, and other water rights issues. Flow statistics could be needed at any location along a stream. Most often, streamflow statistics are needed at ungaged sites, where no flow data are available to compute the statistics. Methods are presented in this report for estimating flow-duration and annual mean-flow statistics for ungaged streams in Oklahoma. Flow statistics included the (1) annual (period of record), (2) seasonal (summer-autumn and winter-spring), and (3) 12 monthly duration statistics, including the 20th, 50th, 80th, 90th, and 95th percentile flow exceedances, and the annual mean-flow (mean of daily flows for the period of record). Flow statistics were calculated from daily streamflow information collected from 235 streamflow-gaging stations throughout Oklahoma and areas in adjacent states. A drainage-area ratio method is the preferred method for estimating flow statistics at an ungaged location that is on a stream near a gage. The method generally is reliable only if the drainage-area ratio of the two sites is between 0.5 and 1.5. Regression equations that relate flow statistics to drainage-basin characteristics were developed for the purpose of estimating selected flow-duration and annual mean-flow statistics for ungaged streams that are not near gaging stations on the same stream. Regression equations were developed from flow statistics and drainage-basin characteristics for 113 unregulated gaging stations. Separate regression equations were developed by using U.S. Geological Survey streamflow-gaging stations in regions with similar drainage-basin characteristics. These equations can increase the accuracy of regression equations used for estimating flow-duration and annual mean-flow statistics at ungaged stream locations in Oklahoma. Streamflow-gaging stations were grouped by selected drainage

  13. Flow cytometric and laser scanning microscopic approaches in epigenetics research.

    PubMed

    Szekvolgyi, Lorant; Imre, Laszlo; Minh, Doan Xuan Quang; Hegedus, Eva; Bacso, Zsolt; Szabo, Gabor

    2009-01-01

    Our understanding of epigenetics has been transformed in recent years by the advance of technological possibilities based primarily on a powerful tool, chromatin immunoprecipitation (ChIP). However, in many cases, the detection of epigenetic changes requires methods providing a high-throughput (HTP) platform. Cytometry has opened a novel approach for the quantitative measurement of molecules, including PCR products, anchored to appropriately addressed microbeads (Pataki et al. 2005. Cytometry 68, 45-52). Here we show selected examples for the utility of two different cytometry-based platforms of epigenetic analysis: ChIP-on-beads, a flow-cytometric test of local histone modifications (Szekvolgyi et al. 2006. Cytometry 69, 1086-1091), and the laser scanning cytometry-based measurement of global epigenetic modifications that might help predict clinical behavior in different pathological conditions. We anticipate that such alternative tools may shortly become indispensable in clinical practice, translating the systematic screening of epigenetic tags from basic research into routine diagnostics of HTP demand.

  14. A Finite Element Method for Simulation of Compressible Cavitating Flows

    NASA Astrophysics Data System (ADS)

    Shams, Ehsan; Yang, Fan; Zhang, Yu; Sahni, Onkar; Shephard, Mark; Oberai, Assad

    2016-11-01

    This work focuses on a novel approach for finite element simulations of multi-phase flows which involve evolving interface with phase change. Modeling problems, such as cavitation, requires addressing multiple challenges, including compressibility of the vapor phase, interface physics caused by mass, momentum and energy fluxes. We have developed a mathematically consistent and robust computational approach to address these problems. We use stabilized finite element methods on unstructured meshes to solve for the compressible Navier-Stokes equations. Arbitrary Lagrangian-Eulerian formulation is used to handle the interface motions. Our method uses a mesh adaptation strategy to preserve the quality of the volumetric mesh, while the interface mesh moves along with the interface. The interface jump conditions are accurately represented using a discontinuous Galerkin method on the conservation laws. Condensation and evaporation rates at the interface are thermodynamically modeled to determine the interface velocity. We will present initial results on bubble cavitation the behavior of an attached cavitation zone in a separated boundary layer. We acknowledge the support from Army Research Office (ARO) under ARO Grant W911NF-14-1-0301.

  15. A general method to determine the stability of compressible flows

    NASA Technical Reports Server (NTRS)

    Guenther, R. A.; Chang, I. D.

    1982-01-01

    Several problems were studied using two completely different approaches. The initial method was to use the standard linearized perturbation theory by finding the value of the individual small disturbance quantities based on the equations of motion. These were serially eliminated from the equations of motion to derive a single equation that governs the stability of fluid dynamic system. These equations could not be reduced unless the steady state variable depends only on one coordinate. The stability equation based on one dependent variable was found and was examined to determine the stability of a compressible swirling jet. The second method applied a Lagrangian approach to the problem. Since the equations developed were based on different assumptions, the condition of stability was compared only for the Rayleigh problem of a swirling flow, both examples reduce to the Rayleigh criterion. This technique allows including the viscous shear terms which is not possible in the first method. The same problem was then examined to see what effect shear has on stability.

  16. Rocket nozzle lip flow by direct simulation Monte Carlo method

    NASA Technical Reports Server (NTRS)

    Hueser, J. E.; Brock, F. J.; Melfi, L. T., Jr.; Bird, G. A.

    1985-01-01

    The flow in the immediate vicinity of a rocket nozzle lip has been analyzed, and the results are presented. A rapid change in gas composition is observed in the flow around the lip depending principally on species molecular mass and local flow angle. The divergence of axial and radial temperatures indicating breakdown of translational equilibrium is observed in the results.

  17. An in-well heat-tracer-test method for evaluating borehole flow conditions

    NASA Astrophysics Data System (ADS)

    Sellwood, Stephen M.; Hart, David J.; Bahr, Jean M.

    2015-12-01

    An improved method is presented for characterizing vertical borehole flow conditions in open boreholes using in-well heat tracer tests monitored by a distributed temperature sensing (DTS) system. This flow logging method uses an electrical resistance heater to warm slugs of water within bedrock boreholes and DTS monitoring of subsequent heat migration to measure borehole flow characteristics. Use of an electrical resistance heater allows for controlled test initiation, while the DTS allows for detailed monitoring of heat movement within the borehole. The method was evaluated in bedrock boreholes open to Cambrian sandstone formations in south-central Wisconsin (USA). The method was successfully used to measure upward flow, downward flow, and zero flow, and to identify changes in borehole flow rates associated with fracture flow and porous media flow. The main benefits of the DTS-monitored in-well heat tracer test method of borehole flow logging are (1) borehole flow direction and changes in borehole fluid velocity are readily apparent from a simple plot of the field data, (2) the case of zero vertical borehole flow is easily and confidently identified, and (3) the ability to monitor temperatures over the full borehole length simultaneously and in rapid succession provides detailed flow data with minimal disturbance of the borehole flow. The results of this study indicate that DTS-monitored in-well heat tracer tests are an effective method of characterizing borehole flow conditions.

  18. A proposed through-flow inverse method for the design of mixed-flow pumps

    NASA Technical Reports Server (NTRS)

    Borges, Joao Eduardo

    1991-01-01

    A through-flow (hub-to-shroud) truly inverse method is proposed and described. It uses an imposition of mean swirl, i.e., radius times mean tangential velocity, given throughout the meridional section of the turbomachine as an initial design specification. In the present implementation, it is assumed that the fluid is inviscid, incompressible, and irrotational at inlet and that the blades are supposed to have zero thickness. Only blade rows that impart to the fluid a constant work along the space are considered. An application of this procedure to design the rotor of a mixed-flow pump is described in detail. The strategy used to find a suitable mean swirl distribution and the other design inputs is also described. The final blade shape and pressure distributions on the blade surface are presented, showing that it is possible to obtain feasible designs using this technique. Another advantage of this technique is the fact that it does not require large amounts of CPU time.

  19. DIFFEOMORPHIC SURFACE FLOWS: A NOVEL METHOD OF SURFACE EVOLUTION.

    PubMed

    Zhang, Sirong; Younes, Laurent; Zweck, John; Ratnanather, J Tilak

    2008-01-01

    We describe a new class of surface flows, diffeomorphic surface flows, induced by restricting diffeomorphic flows of the ambient Euclidean space to a surface. Different from classical surface PDE flows such as mean curvature flow, diffeomorphic surface flows are solutions of integro-differential equations in a group of diffeomorphisms. They have the potential advantage of being both topology-invariant and singularity free, which can be useful in computational anatomy and computer graphics. We first derive the Euler-Lagrange equation of the elastic energy for general diffeomorphic surface flows, which can be regarded as a smoothed version of the corresponding classical surface flows. Then we focus on diffeomorphic mean curvature flow. We prove the short-time existence and uniqueness of the flow, and study the long-time existence of the flow for surfaces of revolution. We present numerical experiments on synthetic and cortical surfaces from neuroimaging studies in schizophrenia and auditory disorders. Finally we discuss unresolved issues and potential applications.

  20. DIFFEOMORPHIC SURFACE FLOWS: A NOVEL METHOD OF SURFACE EVOLUTION*

    PubMed Central

    Zhang, Sirong; Younes, Laurent; Zweck, John; Ratnanather, J. Tilak

    2009-01-01

    We describe a new class of surface flows, diffeomorphic surface flows, induced by restricting diffeomorphic flows of the ambient Euclidean space to a surface. Different from classical surface PDE flows such as mean curvature flow, diffeomorphic surface flows are solutions of integro-differential equations in a group of diffeomorphisms. They have the potential advantage of being both topology-invariant and singularity free, which can be useful in computational anatomy and computer graphics. We first derive the Euler–Lagrange equation of the elastic energy for general diffeomorphic surface flows, which can be regarded as a smoothed version of the corresponding classical surface flows. Then we focus on diffeomorphic mean curvature flow. We prove the short-time existence and uniqueness of the flow, and study the long-time existence of the flow for surfaces of revolution. We present numerical experiments on synthetic and cortical surfaces from neuroimaging studies in schizophrenia and auditory disorders. Finally we discuss unresolved issues and potential applications. PMID:20016768

  1. Preconditioned Conjugate Gradient methods for low speed flow calculations

    NASA Technical Reports Server (NTRS)

    Ajmani, Kumud; Ng, Wing-Fai; Liou, Meng-Sing

    1993-01-01

    An investigation is conducted into the viability of using a generalized Conjugate Gradient-like method as an iterative solver to obtain steady-state solutions of very low-speed fluid flow problems. Low-speed flow at Mach 0.1 over a backward-facing step is chosen as a representative test problem. The unsteady form of the two dimensional, compressible Navier-Stokes equations are integrated in time using discrete time-steps. The Navier-Stokes equations are cast in an implicit, upwind finite-volume, flux split formulation. The new iterative solver is used to solve a linear system of equations at each step of the time-integration. Preconditioning techniques are used with the new solver to enhance the stability and the convergence rate of the solver and are found to be critical to the overall success of the solver. A study of various preconditioners reveals that a preconditioner based on the lower-upper (L-U)-successive symmetric over-relaxation iterative scheme is more efficient than a preconditioner based on incomplete L-U factorizations of the iteration matrix. The performance of the new preconditioned solver is compared with a conventional line Gauss-Seidel relaxation (LGSR) solver. Overall speed-up factors of 28 (in terms of global time-steps required to converge to a steady-state solution) and 20 (in terms of total CPU time on one processor of a CRAY-YMP) are found in favor of the new preconditioned solver, when compared with the LGSR solver.

  2. Consistent lattice Boltzmann methods for incompressible axisymmetric flows

    NASA Astrophysics Data System (ADS)

    Zhang, Liangqi; Yang, Shiliang; Zeng, Zhong; Yin, Linmao; Zhao, Ya; Chew, Jia Wei

    2016-08-01

    In this work, consistent lattice Boltzmann (LB) methods for incompressible axisymmetric flows are developed based on two efficient axisymmetric LB models available in the literature. In accord with their respective original models, the proposed axisymmetric models evolve within the framework of the standard LB method and the source terms contain no gradient calculations. Moreover, the incompressibility conditions are realized with the Hermite expansion, thus the compressibility errors arising in the existing models are expected to be reduced by the proposed incompressible models. In addition, an extra relaxation parameter is added to the Bhatnagar-Gross-Krook collision operator to suppress the effect of the ghost variable and thus the numerical stability of the present models is significantly improved. Theoretical analyses, based on the Chapman-Enskog expansion and the equivalent moment system, are performed to derive the macroscopic equations from the LB models and the resulting truncation terms (i.e., the compressibility errors) are investigated. In addition, numerical validations are carried out based on four well-acknowledged benchmark tests and the accuracy and applicability of the proposed incompressible axisymmetric LB models are verified.

  3. Devices and methods of operation thereof for providing stable flow for centrifugal compressors

    NASA Technical Reports Server (NTRS)

    Skoch, Gary J. (Inventor); Stevens, Mark A. (Inventor); Jett, Thomas A. (Inventor)

    2008-01-01

    Centrifugal compressor flow stabilizing devices and methods of operation thereof are disclosed that act upon the flow field discharging from the impeller of a centrifugal compressor and modify the flow field ahead of the diffuser vanes such that flow conditions contributing to rotating stall and surge are reduced or even eliminated. In some embodiments, shaped rods and methods of operation thereof are disclosed, whereas in other embodiments reverse-tangent air injection devices and methods are disclosed.

  4. Analysis of methods to estimate spring flows in a karst aquifer

    USGS Publications Warehouse

    Sepulveda, N.

    2009-01-01

    Hydraulically and statistically based methods were analyzed to identify the most reliable method to predict spring flows in a karst aquifer. Measured water levels at nearby observation wells, measured spring pool altitudes, and the distance between observation wells and the spring pool were the parameters used to match measured spring flows. Measured spring flows at six Upper Floridan aquifer springs in central Florida were used to assess the reliability of these methods to predict spring flows. Hydraulically based methods involved the application of the Theis, Hantush-Jacob, and Darcy-Weisbach equations, whereas the statistically based methods were the multiple linear regressions and the technology of artificial neural networks (ANNs). Root mean square errors between measured and predicted spring flows using the Darcy-Weisbach method ranged between 5% and 15% of the measured flows, lower than the 7% to 27% range for the Theis or Hantush-Jacob methods. Flows at all springs were estimated to be turbulent based on the Reynolds number derived from the Darcy-Weisbach equation for conduit flow. The multiple linear regression and the Darcy-Weisbach methods had similar spring flow prediction capabilities. The ANNs provided the lowest residuals between measured and predicted spring flows, ranging from 1.6% to 5.3% of the measured flows. The model prediction efficiency criteria also indicated that the ANNs were the most accurate method predicting spring flows in a karst aquifer. ?? 2008 National Ground Water Association.

  5. Undergraduate Contributions to Developing New Methods for Analyzing Blood Flows

    NASA Astrophysics Data System (ADS)

    Lowe, Mary L.

    1998-11-01

    At Loyola, undergraduate research is strongly encouraged for students at every stage in college. Since 1988, I have supervised undergraduates with different majors, different physics backgrounds, different grade point averages, and different maturity levels. Their contributions to three experiments will be described. The first student measured the index of refraction of the walls of a tube used to study flows in curved, macroscopic ducts at Reynolds numbers matching arterial blood flow. A second student made preliminary measurements of flows in microfabricated venular bifurcations using confocal microscopy. A third group of students tested optical flow algorithms on digital images of flows in microscopic tubes with latex beads as tracers. This led to velocimetry studies of blood flows in vivo and in vitro.

  6. Innovative Method for Greatly Reducing Flow Resistance and Obtaining Well-Ordered Continuous Flow

    NASA Astrophysics Data System (ADS)

    Lin, Weiyi

    2009-11-01

    In this paper, firstly, the experiment on the flow resistance of the aerated pipe flow is introduced. And some experimental research on comparison between different volumes of air entrained is presented. Secondly, the characteristics of Gravity Pipe Flow under the action of Torricelli's Vacuum, shortly called as GPFUTV are dissertated, including creative and functional design, fundamental principle, etc. Under GPFUTV condition the water flow in the tube is full-pipe and continuous, colorless and non-aerated, high-speed and non-rotational as distinguished from laminar flow. Thirdly, an appeal in relation to the experimental research, the applied studies and basic theory research is given. For instance, the well-known Reynolds' experiment under GPFUTV condition, the potential for GPFUTV to be developed for deep seawater suction technology, seawater intake pipe of OTEC and lifting technology for deep ocean mining in Fe-Mn concretions, flow stability and flow resistance under GPFUTV condition, etc.

  7. A locally implicit method for fluid flow problems

    NASA Technical Reports Server (NTRS)

    Reddy, K. C.

    1986-01-01

    The fluid flow inside the space shuttle main engine (SSME) traverses through a complex geometrical configuration. The flow is compressible, viscous, and turbulent with pockets of separated regions. Several computer codes are being developed to solve three dimensional Navier-Stokes equations with different turbulence models for analyzing the SSME internal flow. The locally implicit scheme is a computationally efficient scheme which converges rapidly in multi-grid modes for elliptic problems. It has the promise of providing a rapidly converging algorithm for steady-state viscous flow problems.

  8. ISS Payload Racks Automated Flow Control Calibration Method

    NASA Technical Reports Server (NTRS)

    Simmonds, Boris G.

    2003-01-01

    Payload Racks utilize MTL and/or LTL station water for cooling of payloads and avionics. Flow control range from valves of fully closed, to up to 300 Ibmhr. Instrument accuracies are as high as f 7.5 Ibm/hr for flow sensors and f 3 Ibm/hr for valve controller, for a total system accuracy of f 10.5 Ibm/hr. Improved methodology was developed, tested and proven that reduces accuracy of the commanded flows to less than f 1 Ibmhr. Uethodology could be packed in a "calibration kit" for on- orbit flow sensor checkout and recalibration, extending the rack operations before return to earth. -

  9. A study of nozzle and ejector flow problems by the method of integral relations

    NASA Technical Reports Server (NTRS)

    Howlett, L. D.; Chow, W. L.

    1972-01-01

    The application of the method of integral relations to nozzle and ejector flow problems was examined. For nozzle flow problems, the general formulation is that the approaching flow may be rotational. Particular attention was given to the phenomenon of choking under nonuniform flow conditions. Numerical integration of the governing ordinary differential equations was also investigated. This scheme of analysis was also applied to study the interacting flow field within an ejector system.

  10. Numerical simulation of the non-Newtonian fluid flow using the indirect boundary element method

    NASA Astrophysics Data System (ADS)

    Bessonova, M. P.; Yakutenok, V. A.

    2017-02-01

    The indirect boundary element method is formulated for a two-dimensional Stokes flow with the moving boundary when gravity force aids the flow. The governing equations of low Reynolds flow are formulated. The numerical technique is described. Two regimes of the fluid flow depending on the Stokes number value were detected: the regime of full filling and the jet flow regime. The comparison of obtained results with data of other authors is presented.

  11. Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry

    PubMed Central

    Pandey, Gouri Shankar; Tseng, Sandra C.; Howard, Tom E.; Sauna, Zuben E.

    2013-01-01

    Flow cytometry is widely used in cancer research for diagnosis, detection of minimal residual disease, as well as immune monitoring and profiling following immunotherapy. Detection of specific host proteins for diagnosis predominantly uses quantitative PCR and western blotting assays. In this study, we optimized a flow cytometry-based detection assay for Factor VIII protein in peripheral blood mononuclear cells (PBMCs). An indirect intracellular staining (ICS) method was standardized using monoclonal antibodies to different domains of human Factor VIII protein. The FVIII protein expression level was estimated by calculating the mean and median fluorescence intensities (MFI) values for each monoclonal antibody. ICS staining of transiently transfected cell lines supported the method's specificity. Intracellular FVIII protein expression was also detected by the monoclonal antibodies used in the study in PBMCs of five blood donors. In summary, our data suggest that intracellular FVIII detection in PBMCs of hemophilia A patients can be a rapid and reliable method to detect intracellular FVIII levels. PMID:23555096

  12. An efficient method for DEM-based overland flow routing

    NASA Astrophysics Data System (ADS)

    Huang, Pin-Chun; Lee, Kwan Tun

    2013-05-01

    The digital elevation model (DEM) is frequently used to represent watershed topographic features based on a raster or a vector data format. It has been widely linked with flow routing equations for watershed runoff simulation. In this study, a recursive formulation was encoded into the conventional kinematic- and diffusion-wave routing algorithms to permit a larger time increment, despite the Courant-Friedrich-Lewy condition having been violated. To meet the requirement of recursive formulation, a novel routing sequence was developed to determine the cell-to-cell computational procedure for the DEM database. The routing sequence can be set either according to the grid elevation in descending order for the kinematic-wave routing or according to the water stage of the grid in descending order for the diffusion-wave routing. The recursive formulation for 1D runoff routing was first applied to a conceptual overland plane to demonstrate the precision of the formulation using an analytical solution for verification. The proposed novel routing sequence with the recursive formulation was then applied to two mountain watersheds for 2D runoff simulations. The results showed that the efficiency of the proposed method was significantly superior to that of the conventional algorithm, especially when applied to a steep watershed.

  13. Axial and Centrifugal Compressor Mean Line Flow Analysis Method

    NASA Technical Reports Server (NTRS)

    Veres, Joseph P.

    2009-01-01

    This paper describes a method to estimate key aerodynamic parameters of single and multistage axial and centrifugal compressors. This mean-line compressor code COMDES provides the capability of sizing single and multistage compressors quickly during the conceptual design process. Based on the compressible fluid flow equations and the Euler equation, the code can estimate rotor inlet and exit blade angles when run in the design mode. The design point rotor efficiency and stator losses are inputs to the code, and are modeled at off design. When run in the off-design analysis mode, it can be used to generate performance maps based on simple models for losses due to rotor incidence and inlet guide vane reset angle. The code can provide an improved understanding of basic aerodynamic parameters such as diffusion factor, loading levels and incidence, when matching multistage compressor blade rows at design and at part-speed operation. Rotor loading levels and relative velocity ratio are correlated to the onset of compressor surge. NASA Stage 37 and the three-stage NASA 74-A axial compressors were analyzed and the results compared to test data. The code has been used to generate the performance map for the NASA 76-B three-stage axial compressor featuring variable geometry. The compressor stages were aerodynamically matched at off-design speeds by adjusting the variable inlet guide vane and variable stator geometry angles to control the rotor diffusion factor and incidence angles.

  14. The least-squares finite element method for low-mach-number compressible viscous flows

    NASA Technical Reports Server (NTRS)

    Yu, Sheng-Tao

    1994-01-01

    The present paper reports the development of the Least-Squares Finite Element Method (LSFEM) for simulating compressible viscous flows at low Mach numbers in which the incompressible flows pose as an extreme. Conventional approach requires special treatments for low-speed flows calculations: finite difference and finite volume methods are based on the use of the staggered grid or the preconditioning technique; and, finite element methods rely on the mixed method and the operator-splitting method. In this paper, however, we show that such difficulty does not exist for the LSFEM and no special treatment is needed. The LSFEM always leads to a symmetric, positive-definite matrix through which the compressible flow equations can be effectively solved. Two numerical examples are included to demonstrate the method: first, driven cavity flows at various Reynolds numbers; and, buoyancy-driven flows with significant density variation. Both examples are calculated by using full compressible flow equations.

  15. Innovative method for greatly reducing fluid flow resistance

    NASA Astrophysics Data System (ADS)

    Lin, Weiyi

    2007-11-01

    In this paper, firstly, the aerated pipe flow experiment is introduced. And some experimental research on comparison between different volume of air entrained is presented. Secondly, the technical characteristics of gravity pipe flow under the action of Torricelli's vacuum, shortly called as GPFUTV are dissertated, including creative and functional design, fundamental principle and the strange energy loss phenomena, etc. The detailed information on energy loss of water flow under GPFUTV's condition please find the attached YongAn Water Plant Test Report Auguest 1997 and ShiLong District Reservoir Diversion Project Test Report April 2007. Thirdly, an appeal in relation to the experimental research, the applied studies and basic theory research is given. For instance, Reynolds' experiment and Nikuradze's experiments under GPFUTV's condition, the use of GPFUTV instead of lifting pump in DOW project and deep ocean mining project, flow stability and flow resistance under GPFUTV's condition, etc. At last, the application of GPFUTV in reservoir release control is is illustrated.

  16. Method for flow cytometric monitoring of Renibacterium salmoninarum inactivation

    USGS Publications Warehouse

    Pascho, R.J.; Ongerth, J.E.

    2000-01-01

    with bacteriological culture (r2 ??? 0.22). In both assessments, there was a correlation between the estimates of inactivation based upon HRFI and CS analyses (r2 > 0.99). These results suggest that flow cytometry can be used as a supplementary or alternative method to bacteriological culture for monitoring the inactivation of R. salmoninarum.

  17. A numerical method for a model of two-phase flow in a coupled free flow and porous media system

    NASA Astrophysics Data System (ADS)

    Chen, Jie; Sun, Shuyu; Wang, Xiao-Ping

    2014-07-01

    In this article, we study two-phase fluid flow in coupled free flow and porous media regions. The model consists of coupled Cahn-Hilliard and Navier-Stokes equations in the free fluid region and the two-phase Darcy law in the porous medium region. We propose a Robin-Robin domain decomposition method for the coupled Navier-Stokes and Darcy system with the generalized Beavers-Joseph-Saffman condition on the interface between the free flow and the porous media regions. Numerical examples are presented to illustrate the effectiveness of this method.

  18. Method for controlling coolant flow in airfoil, flow control structure and airfoil incorporating the same

    DOEpatents

    Itzel, Gary Michael; Devine, II, Robert Henry; Chopra, Sanjay; Toornman, Thomas Nelson

    2003-07-08

    A coolant flow control structure is provided to channel cooling media flow to the fillet region defined at the transition between the wall of a nozzle vane and a wall of a nozzle segment, for cooling the fillet region. In an exemplary embodiment, the flow control structure defines a gap with the fillet region to achieve the required heat transfer coefficients in this region to meet part life requirements.

  19. The experimental verification of a streamline curvature numerical analysis method applied to the flow through an axial flow fan

    NASA Technical Reports Server (NTRS)

    Pierzga, M. J.

    1981-01-01

    The experimental verification of an inviscid, incompressible through-flow analysis method is presented. The primary component of this method is an axisymmetric streamline curvature technique which is used to compute the hub-to-tip flow field of a given turbomachine. To analyze the flow field in the blade-to-blade plane of the machine, the potential flow solution of an infinite cascade of airfoils is also computed using a source model technique. To verify the accuracy of such an analysis method an extensive experimental verification investigation was conducted using an axial flow research fan. Detailed surveys of the blade-free regions of the machine along with intra-blade surveys using rotating pressure sensing probes and blade surface static pressure taps provide a one-to-one relationship between measured and predicted data. The results of this investigation indicate the ability of this inviscid analysis method to predict the design flow field of the axial flow fan test rotor to within a few percent of the measured values.

  20. The experimental verification of a streamline curvature numerical analysis method applied to the flow through an axial flow fan

    NASA Technical Reports Server (NTRS)

    Pierzga, M. J.

    1981-01-01

    The experimental verification of an inviscid, incompressible through-flow analysis method is presented. The primary component of this method is an axisymmetric streamline curvature technique which is used to compute the hub-to-tip flow field of a given turbomachine. To analyze the flow field in the blade-to-blade plane of the machine, the potential flow solution of an infinite cascade of airfoils is also computed using a source model technique. To verify the accuracy of such an analysis method an extensive experimental verification investigation was conducted using an axial flow research fan. Detailed surveys of the blade-free regions of the machine along with intra-blade surveys using rotating pressure sensing probes and blade surface static pressure taps provide a one-to-one relationship between measured and predicted data. The results of this investigation indicate the ability of this inviscid analysis method to predict the design flow field of the axial flow fan test rotor to within a few percent of the measured values.

  1. Application of the Moment Method in the Slip and Transition Regime for Microfluidic Flows

    DTIC Science & Technology

    2011-01-01

    L. The non-equilibrium gas flow , or rarefied gas dynamics has been explored extensively for more than a century in association with high- speed high...Computational Methods for Rarefied Flows (Modeles et methodes de calcul des coulements de gaz rarefies ). RTO-EN-AVT-194 14. ABSTRACT Gas flows in...can be used to guide the development of kinetic and macroscopic models for rarefied gas flow . Here t and xi are temporal and spatial coordinates

  2. Piecewise uniform conduction-like flow channels and method therefor

    DOEpatents

    Cummings, Eric B.; Fiechtner, Gregory J.

    2006-02-28

    A low-dispersion methodology for designing microfabricated conduction channels for on-chip electrokinetic-based systems is presented. The technique relies on trigonometric relations that apply for ideal electrokinetic flows, allowing faceted channels to be designed on chips using common drafting software and a hand calculator. Flows are rotated and stretched along the abrupt interface between adjacent regions with differing permeability. Regions bounded by interfaces form flow "prisms" that can be combined with other designed prisms to obtain a wide range of turning angles and expansion ratios while minimizing dispersion. Designs are demonstrated using two-dimensional numerical solutions of the Laplace equation.

  3. A flow quantification method using fluid dynamics regularization and MR tagging.

    PubMed

    Jiraraksopakun, Yuttapong; McDougall, Mary P; Wright, Steven M; Ji, Jim X

    2010-06-01

    This paper presents a new method for improved flow analysis and quantification using MRI. The method incorporates fluid dynamics to regularize the flow quantification from tagged MR images. Specifically, the flow quantification is formulated as a minimization problem based on the following: 1) the Navier-Stokes equation governing the fluid dynamics; 2) the flow continuity equation and boundary conditions; and 3) the data consistency constraint. The minimization is carried out using a genetic algorithm. This method is tested using both computer simulations and MR flow experiments. The results are evaluated using flow vector fields from the computational fluid dynamics software package as a reference, which show that the new method can achieve more realistic and accurate flow quantifications than the conventional method.

  4. Analysis of lymph flow in microvessels by biomicroscopic and coherent optical methods

    NASA Astrophysics Data System (ADS)

    Galanzha, Ekateryna I.; Brill, Gregory E.; Tuchin, Valery V.; Ulyanov, Sergey S.; Solov'eva, Anastasiya V.; Sedykh, Alexey V.

    2000-04-01

    The lymph flow velocity in microvessels of rat mesentery was investigated by using biomicroscopic manual frame-by-frame technique with image processing system and speckle- interferometrical method. Lymph flow was observed in the majority of lymphatics. Lymph flow was registered in microvessels with phasic contractions and functioning valves. The lymphocytes motion in flow was measured. It was established that mean lymph flow velocity increased in vessels with phasic contractions and moderate concentration of lymphocytes in flow. The application of speckle- interferometry allowed us to estimate lymph flow on the whole with all stops, the increases and decreases of flow velocity. The parameters both of changes of a mean velocity and of spatial-temporal velocity distribution in lymph flow were calculated. Temporal changes of the parameter V showed a shuttle-type character of lymph flow velocity.

  5. Method and applications of fiber synthesis using laminar flow

    NASA Astrophysics Data System (ADS)

    Burns, Bradley Justin

    A Laminar Flow Reactor (LFR) using the principles of hydrodynamic focusing was created and used to fabricate functional composite polymer fibers. These fibers had the ability to conduct or serve as a carrier for singlet oxygen-generating molecules. Critical to the process was designing an easy-to-fabricate, inexpensive device and developing a repeatable method that made efficient use of the materials. The initial designs used a planar layout and hydrodynamically focused in only one dimension while later versions switched to a two-fluid concentric design. Modeling was undertaken and verified for the different device layouts. Three types of conductive particles were embedded in the formed polymer: silver, indium tin oxide (ITO) and polyaniline. The polymer was also used as a carrier to two singlet oxygen generating molecules: Methylene Blue (MB) and perylene. Both were effective in killing Bacillus thuringiensis but MB leached from the fiber into the tested cell suspension. Perylene, which is not water soluble, did not leach out and was just as effective as MB. Research that was performed at ITT is also presented. A critical need exists to detect, identify, quantify, locate, and track virus and toxin aerosols to provide early warning during both light and dark conditions. The solution presented is a remote sensing technology using seeding particles. Seeding particles developed during this program provide specific identification of threat cloud content. When introduced to the threat cloud the seeders will bind specifically to the analyte of interest and upon interrogation from a stand off laser source will fluoresce. The fluorescent signal is detected from a distance using a long-range microscope and collection optics that allow detection of low concentrations of threat aerosols.

  6. A reduced complexity numerical method for flow accumulation and sediment transport under both convergent and divergent flow conditions

    NASA Astrophysics Data System (ADS)

    Hobley, Daniel; Paola, Chris; Voller, Vaughan

    2017-04-01

    Many methods currently used to model the mobilisation and depositions of sediment in natural environments on medium to long timescales are numerically inefficient. This is in part a function of the complexity of the underlying flow physics, and in part a function of similar complexity in the physics of sediment transport. Here, we derive a new approach to calculate both flow accumulation and resulting sediment transport across a grid by adopting a novel, simplified, vectorised scheme to describe the flow of water over terrain. The method for calculating local discharge assumes that the energy gradient of the flow is adequately represented by the bed gradient (removing flow dependence on the acceleration or momentum of the flow), then enforces only: 1. conservation of water mass, and 2. that flow is always perpendicular to slope. Sediment transport can be added to the scheme by introducing a pair of additional assumptions: 3. that sediment mass is conserved (i.e., the Exner equation), and 4. that volume flux of sediment is some defined function of local discharge and bed gradient. The method is equivalent to solving for a "flow potential" vector field across the grid, which is always follows with the local bed gradient. The resulting matrix solution is order n and highly efficient, both for flow routing alone and also when calculating the resulting sediment transport. Here, the model is demonstrated as part of the Landlab modelling framework, but it would be equally well suited to integration into any other gridded surface process model. The method is validated by comparison to results from physical experiments describing fan and delta top evolution performed at the National Center for Earth Surface Dynamics.

  7. Computational Methods for Feedback Controllers for Aerodynamics Flow Applications

    DTIC Science & Technology

    2007-08-15

    include the massively separated flow around an F-1i5E at 650 angle of attack reported by Forsythe et a/. (2004) (the first eddy-resolving simulation...to one step prediction, of MIMO (multi-input, multi- output) systems. A schematic representation of the feed-forward ANN-ARX network topology is...present, and can also accommodate multiple actuator interaction allowing for MIMO control. We did not intend it to be used for random turbulent flows, but

  8. An advanced panel method for analysis of arbitrary configurations in unsteady subsonic flow

    NASA Technical Reports Server (NTRS)

    Dusto, A. R.; Epton, M. A.

    1980-01-01

    An advanced method is presented for solving the linear integral equations for subsonic unsteady flow in three dimensions. The method is applicable to flows about arbitrary, nonplanar boundary surfaces undergoing small amplitude harmonic oscillations about their steady mean locations. The problem is formulated with a wake model wherein unsteady vorticity can be convected by the steady mean component of flow. The geometric location of the unsteady source and doublet distributions can be located on the actual surfaces of thick bodies in their steady mean locations. The method is an outgrowth of a recently developed steady flow panel method and employs the linear source and quadratic doublet splines of that method.

  9. Plasma dragged microparticles as a method to measure plasma flows

    SciTech Connect

    Ticos, Catalin M.; Wang Zhehui; Delzanno, Gian Luca; Lapenta, Giovanni

    2006-10-15

    The physics of microparticle motion in flowing plasmas is studied in detail for plasmas with electron and ion densities n{sub e,i}{approx}10{sup 19} m{sup -3}, electron and ion temperatures of no more than 15 eV, and plasma flows on the order of the ion thermal speed, v{sub f}{approx}v{sub ti}. The equations of motion due to Coulomb interactions and direct impact with ions and electrons, of charge variation, as well as of heat exchange with the plasma, are solved numerically for isolated particles (or dust grains) of micron sizes. It is predicted that microparticles can survive in plasma long enough, and can be dragged in the direction of the local ion flow. Based on the theoretical analysis, we describe a new plasma flow measurement technique called microparticle tracer velocimetry (mPTV), which tracks microparticle motion in a plasma with a high-speed camera. The mPTV can reveal the directions of the plasma flow vectors at multiple locations simultaneously and at submillimeter scales, which is hard to achieve by most other techniques. Thus, mPTV can be used to study plasma flows produced in the laboratory.

  10. Development of a flow rate monitoring method for the wearable ventricular assist device driver.

    PubMed

    Ohnuma, Kentaro; Homma, Akihiko; Sumikura, Hirohito; Tsukiya, Tomonori; Takewa, Yoshiaki; Mizuno, Toshihide; Mukaibayashi, Hiroshi; Kojima, Koichi; Katano, Kazuo; Taenaka, Yoshiyuki; Tatsumi, Eisuke

    2015-06-01

    Our research institute has been working on the development of a compact wearable drive unit for an extracorporeal ventricular assist device (VAD) with a pneumatically driven pump. A method for checking the pump blood flow on the side of the drive unit without modifying the existing blood pump and impairing the portability of it will be useful. In this study, to calculate the pump flow rate indirectly from measuring the flow rate of the driving air of the VAD air chamber, we conducted experiments using a mock circuit to investigate the correlation between the air flow rate and the pump flow rate as well as its accuracy and error factors. The pump flow rate was measured using an ultrasonic flow meter at the inflow and outflow tube, and the air flow was measured using a thermal mass flow meter at the driveline. Similarity in the instantaneous waveform was confirmed between the air flow rate in the driveline and the pump flow rate. Some limitations of this technique were indicated by consideration of the error factors. A significant correlation was found between the average pump flow rate in the ejecting direction and the average air flow rate in the ejecting direction (R2 = 0.704-0.856), and the air flow rate in the filling direction (R2 = 0.947-0.971). It was demonstrated that the average pump flow rate was estimated exactly in a wide range of drive conditions using the air flow of the filling phase.

  11. High-order discontinuous Galerkin method for applications to multicomponent and chemically reacting flows

    NASA Astrophysics Data System (ADS)

    Lv, Yu; Ihme, Matthias

    2017-06-01

    This article focuses on the development of a discontinuous Galerkin (DG) method for simulations of multicomponent and chemically reacting flows. Compared to aerodynamic flow applications, in which DG methods have been successfully employed, DG simulations of chemically reacting flows introduce challenges that arise from flow unsteadiness, combustion, heat release, compressibility effects, shocks, and variations in thermodynamic properties. To address these challenges, algorithms are developed, including an entropy-bounded DG method, an entropy-residual shock indicator, and a new formulation of artificial viscosity. The performance and capabilities of the resulting DG method are demonstrated in several relevant applications, including shock/bubble interaction, turbulent combustion, and detonation. It is concluded that the developed DG method shows promising performance in application to multicomponent reacting flows. The paper concludes with a discussion of further research needs to enable the application of DG methods to more complex reacting flows.

  12. Experimental verification of color flow imaging based on wideband Doppler method.

    PubMed

    Tanaka, Naohiko

    2014-01-01

    The purpose of this study is to eliminate the aliasing in color flow imaging. The wideband Doppler method is applied to generate a color flow image, and the validity of the method is experimentally confirmed. The single beam experiment is carried out to confirm the velocity estimation based on the wideband Doppler method. The echo data for the conventional pulsed Doppler method and the wideband Doppler method are obtained using a flow model, and the estimated velocity for each method is compared. The color flow images for each method are also generated using several types of flow model. The generated images are compared, and the characteristics of the imaging based on the wideband Doppler method are discussed. The high velocity beyond the Nyquist limit is successfully estimated by the wideband Doppler method, and the availability in low velocity estimation is also confirmed. The aliasing in color flow images is eliminated, and the generated images show the significance of the elimination of the aliasing in the flow imaging. The aliasing in color flow imaging can be eliminated by the wideband Doppler method. This technique is useful for the exact understanding of blood flow dynamics.

  13. The CABARET method for a weakly compressible fluid flows in one- and two-dimensional implementations

    NASA Astrophysics Data System (ADS)

    Kulikov, Yu M.; Son, E. E.

    2016-11-01

    The CABARET method implementation for a weakly compressible fluid flow is in the focus of present paper. Testing both one-dimensional pressure balancing problem and a classical plane Poiseuille flow, we analyze this method in terms of discontinuity resolution, dispersion and dissipation. The method is proved to have an adequate convergence to an analytical solution for a velocity profile. We also show that a flow formation process represents a set of self-similar solutions under varying pressure differential and sound speed.

  14. An air traffic flow management method based on mixed genetic algorithms

    NASA Astrophysics Data System (ADS)

    Fu, Ying

    2009-12-01

    With the air traffic congest problem becoming more and more severe, the study of air traffic flow management is more and more important. According to the character of air traffic flow management, the author analyzed the heuristic method and genetic algorithms, later put this two method together and give a new method of air traffic flow management-mixture genetic algorithms, It has global convergence, the simulation result demonstrates that the presented algorithm is effective.

  15. Lattice Boltzmann Method for 3-D Flows with Curved Boundary

    NASA Technical Reports Server (NTRS)

    Mei, Renwei; Shyy, Wei; Yu, Dazhi; Luo, Li-Shi

    2002-01-01

    In this work, we investigate two issues that are important to computational efficiency and reliability in fluid dynamics applications of the lattice, Boltzmann equation (LBE): (1) Computational stability and accuracy of different lattice Boltzmann models and (2) the treatment of the boundary conditions on curved solid boundaries and their 3-D implementations. Three athermal 3-D LBE models (D3QI5, D3Ql9, and D3Q27) are studied and compared in terms of efficiency, accuracy, and robustness. The boundary treatment recently developed by Filippova and Hanel and Met et al. in 2-D is extended to and implemented for 3-D. The convergence, stability, and computational efficiency of the 3-D LBE models with the boundary treatment for curved boundaries were tested in simulations of four 3-D flows: (1) Fully developed flows in a square duct, (2) flow in a 3-D lid-driven cavity, (3) fully developed flows in a circular pipe, and (4) a uniform flow over a sphere. We found that while the fifteen-velocity 3-D (D3Ql5) model is more prone to numerical instability and the D3Q27 is more computationally intensive, the 63Q19 model provides a balance between computational reliability and efficiency. Through numerical simulations, we demonstrated that the boundary treatment for 3-D arbitrary curved geometry has second-order accuracy and possesses satisfactory stability characteristics.

  16. A Flow-Map Method of Representing Cognitive Structure Based on Respondents' Narrative Using Science Content.

    ERIC Educational Resources Information Center

    Anderson, O. Roger; Demetrius, Olive J.

    1993-01-01

    Method is presented for displaying the sequential and multirelational ideation of scientific narrative elicited from respondents. The flow map provides a figural representation of the flow of information, the points in the flow where multirelational and recurrent linkages are made, and the time required to retrieve and express the information at…

  17. Numerical methods for simulating blood flow at macro, micro, and multi scales.

    PubMed

    Imai, Yohsuke; Omori, Toshihiro; Shimogonya, Yuji; Yamaguchi, Takami; Ishikawa, Takuji

    2016-07-26

    In the past decade, numerical methods for the computational biomechanics of blood flow have progressed to overcome difficulties in diverse applications from cellular to organ scales. Such numerical methods may be classified by the type of computational mesh used for the fluid domain, into fixed mesh methods, moving mesh (boundary-fitted mesh) methods, and mesh-free methods. The type of computational mesh used is closely related to the characteristics of each method. We herein provide an overview of numerical methods recently used to simulate blood flow at macro and micro scales, with a focus on computational meshes. We also discuss recent progress in the multi-scale modeling of blood flow.

  18. Method and apparatus for adapting steady flow with cyclic thermodynamics

    DOEpatents

    Swift, Gregory W.; Reid, Robert S.; Ward, William C.

    2000-01-01

    Energy transfer apparatus has a resonator for supporting standing acoustic waves at a selected frequency with a steady flow process fluid thermodynamic medium and a solid medium having heat capacity. The fluid medium and the solid medium are disposed within the resonator for thermal contact therebetween and for relative motion therebetween. The relative motion is produced by a first means for producing a steady velocity component and second means for producing an oscillating velocity component at the selected frequency and concomitant wavelength of the standing acoustic wave. The oscillating velocity and associated oscillating pressure component provide energy transfer between the steady flow process fluid and the solid medium as the steady flow process fluid moves through the resonator.

  19. A new method for flow rate measurement in millimeter-scale pipes.

    PubMed

    Ji, Haifeng; Gao, Xuemin; Wang, Baoliang; Huang, Zhiyao; Li, Haiqing

    2013-01-25

    Combining the Capacitively Coupled Contactless Conductivity Detection (C4D) technique and the principle of cross correlation flow measurement, a new method for flow rate measurement in millimeter-scale pipes was proposed. The research work included two parts. First, a new five-electrode C4D sensor was developed. Second, with two conductivity signals obtained by the developed sensor, the flow rate measurement was implemented by using the principle of cross correlation flow measurement. The experimental results showed that the proposed flow rate measurement method was effective, the developed five-electrode C4D sensor was successful, and the measurement accuracy was satisfactory. In five millimeter-scale pipes with different inner diameters of 0.5, 0.8, 1.8, 3.0 and 3.9 mm respectively, the maximum relative difference of the flow rate measurement between the reference flow rate and the measured flow rate was less than 5%.

  20. A New Method for Flow Rate Measurement in Millimeter-Scale Pipes

    PubMed Central

    Ji, Haifeng; Gao, Xuemin; Wang, Baoliang; Huang, Zhiyao; Li, Haiqing

    2013-01-01

    Combining the Capacitively Coupled Contactless Conductivity Detection (C4D) technique and the principle of cross correlation flow measurement, a new method for flow rate measurement in millimeter-scale pipes was proposed. The research work included two parts. First, a new five-electrode C4D sensor was developed. Second, with two conductivity signals obtained by the developed sensor, the flow rate measurement was implemented by using the principle of cross correlation flow measurement. The experimental results showed that the proposed flow rate measurement method was effective, the developed five-electrode C4D sensor was successful, and the measurement accuracy was satisfactory. In five millimeter-scale pipes with different inner diameters of 0.5, 0.8, 1.8, 3.0 and 3.9 mm respectively, the maximum relative difference of the flow rate measurement between the reference flow rate and the measured flow rate was less than 5%. PMID:23353139

  1. A direct-inverse method for transonic and separated flows about airfoils

    NASA Technical Reports Server (NTRS)

    Carlson, Leland A.

    1990-01-01

    A direct-inverse technique and computer program called TAMSEP that can be used for the analysis of the flow about airfoils at subsonic and low transonic freestream velocities is presented. The method is based upon a direct-inverse nonconservative full potential inviscid method, a Thwaites laminar boundary layer technique, and the Barnwell turbulent momentum integral scheme; and it is formulated using Cartesian coordinates. Since the method utilizes inverse boundary conditions in regions of separated flow, it is suitable for predicting the flow field about airfoils having trailing edge separated flow under high lift conditions. Comparisons with experimental data indicate that the method should be a useful tool for applied aerodynamic analyses.

  2. A Lagged Diffusivity Method for Computing Total Variation Regularized Fluid Flow

    SciTech Connect

    Basnayake, R.; Luttman, A.; Bollt, E.

    2013-03-01

    There is a great deal of recent work using optical flow methods for analyzing the dynamics of fluids, and much attention has been paid to developing regularization schemes for variational approaches that are consistent with the physics of fluid flow. In this work we show that using total variation to regularize two different kinds of optical flow functionals leads to very good flow field reconstructions for the kinds of dynamical structures that appear in fluid flow. The first optical flow functional is the classical component-based conservation of intensity, and the second approach is to reconstruct the potential of the flow, rather than the flow components. In the two cases, total variation regularization corresponds to imposing different scientific priors on the solution, which we compute using a variation of the Lagged Diffusivity Fixed Point Iteration. Numerical details are presented, and the results are demonstrated on synthetic data and on a data-driven oceanic flow model.

  3. The history of the microsphere method for measuring blood flows with special reference to myocardial blood flow: a personal memoir.

    PubMed

    Hoffman, Julien I E

    2017-04-01

    We use many types of equipment and technologies to make our measurements but give little thought to how they developed. Evolution was once described as a series of recoils from blind alleys, and this is exemplified by the gradual development of the microsphere method of measuring blood flows. The microsphere method is one of the most frequently used methods for measuring blood flow to organs and portions of organs. The method can measure myocardial blood flow with reasonable accuracy (within 10%) down to samples weighing >50 mg but probably will not do so for samples weighing 1-10 mg. Microspheres with diameters from 10 to 15 μm provide the best compromise between accurate flow measurement and retention in tissue. Radioactive labels have been almst entirely replaced by fluorescent labels, but colored microspheres and neutron-activated labels are also used.NEW & NOTEWORTHY The contributions of the various individuals who developed the microsphere method of measuring regional blood flows and how these advances took place are brought to light in this paper.

  4. Computational Methods for Analyzing Fluid Flow Dynamics from Digital Imagery

    SciTech Connect

    Luttman, A.

    2012-03-30

    The main goal (long term) of this work is to perform computational dynamics analysis and quantify uncertainty from vector fields computed directly from measured data. Global analysis based on observed spatiotemporal evolution is performed by objective function based on expected physics and informed scientific priors, variational optimization to compute vector fields from measured data, and transport analysis proceeding with observations and priors. A mathematical formulation for computing flow fields is set up for computing the minimizer for the problem. An application to oceanic flow based on sea surface temperature is presented.

  5. Applications of domain decomposition methods to turbomachinery flows

    NASA Technical Reports Server (NTRS)

    Rai, M. M.

    1988-01-01

    Domain decomposition techniques can be used to great advantage by computational fluid dynamicists in computing flows about complex geometries and adapting the grid to the solution. These techniques are particularly useful in computing flows about several bodies that are in relative motion such as rotor/stator configurations in turbomachinery or helicopter rotor/fuselage configurations. This paper discusses some of the basic ideas involved in transferring information between subdomains in a multidomain calculation and presents results for a simple rotor/stator configuration.

  6. Simple Radiowave-Based Method For Measuring Peripheral Blood Flow Project

    NASA Technical Reports Server (NTRS)

    Oliva-Buisson, Yvette J.

    2014-01-01

    Project objective is to design small radio frequency based flow probes for the measurement of blood flow velocity in peripheral arteries such as the femoral artery and middle cerebral artery. The result will be the technological capability to measure peripheral blood flow rates and flow changes during various environmental stressors such as microgravity without contact to the individual being monitored. This technology may also lead to an easier method of detecting venous gas emboli during extravehicular activities.

  7. Debris flow susceptibility mapping using a qualitative heuristic method and Flow-R along the Yukon Alaska Highway Corridor, Canada

    NASA Astrophysics Data System (ADS)

    Blais-Stevens, A.; Behnia, P.

    2016-02-01

    This research activity aimed at reducing risk to infrastructure, such as a proposed pipeline route roughly parallel to the Yukon Alaska Highway Corridor (YAHC), by filling geoscience knowledge gaps in geohazards. Hence, the Geological Survey of Canada compiled an inventory of landslides including debris flow deposits, which were subsequently used to validate two different debris flow susceptibility models. A qualitative heuristic debris flow susceptibility model was produced for the northern region of the YAHC, from Kluane Lake to the Alaska border, by integrating data layers with assigned weights and class ratings. These were slope angle, slope aspect, surficial geology, plan curvature, and proximity to drainage system. Validation of the model was carried out by calculating a success rate curve which revealed a good correlation with the susceptibility model and the debris flow deposit inventory compiled from air photos, high-resolution satellite imagery, and field verification. In addition, the quantitative Flow-R method was tested in order to define the potential source and debris flow susceptibility for the southern region of Kluane Lake, an area where documented debris flow events have blocked the highway in the past (e.g. 1988). Trial and error calculations were required for this method because there was not detailed information on the debris flows for the YAHC to allow us to define threshold values for some parameters when calculating source areas, spreading, and runout distance. Nevertheless, correlation with known documented events helped define these parameters and produce a map that captures most of the known events and displays debris flow susceptibility in other, usually smaller, steep channels that had not been previously documented.

  8. Debris flow susceptibility mapping using a qualitative heuristic method and Flow-R along the Yukon Alaska Highway Corridor, Canada

    NASA Astrophysics Data System (ADS)

    Blais-Stevens, A.; Behnia, P.

    2015-05-01

    This research activity aimed at reducing risk to infrastructure, such as a proposed pipeline route roughly parallel to the Yukon Alaska Highway Corridor (YAHC) by filling geoscience knowledge gaps in geohazards. Hence, the Geological Survey of Canada compiled an inventory of landslides including debris flow deposits, which were subsequently used to validate two different debris flow susceptibility models. A qualitative heuristic debris flow susceptibility model was produced for the northern region of the YAHC, from Kluane Lake to the Alaska border, by integrating data layers with assigned weights and class ratings. These were slope angle, slope aspect (derived from a 5 m × 5 m DEM), surficial geology, permafrost distribution, and proximity to drainage system. Validation of the model was carried out by calculating a success rate curve which revealed a good correlation with the susceptibility model and the debris flow deposit inventory compiled from air photos, high resolution satellite imagery, and field verification. In addition, the quantitative Flow-R method was tested in order to define the potential source and debris flow susceptibility for the southern region of Kluane Lake, an area where documented debris flow events have blocked the highway in the past (e.g., 1988). Trial and error calculations were required for this method because there was not detailed information on the debris flows for the YAHC to allow us to define threshold values for some parameters when calculating source areas, spreading, and runout distance. Nevertheless, correlation with known documented events helped define these parameters and produce a map that captures most of the known events and displays debris flow susceptibility in other, usually smaller, steep channels that had not been previously documented.

  9. An improved flow cytometry assay to monitor phagosome acidification.

    PubMed

    Colas, Chloé; Menezes, Shinelle; Gutiérrez-Martínez, Enric; Péan, Claire B; Dionne, Marc S; Guermonprez, Pierre

    2014-10-01

    Phago-lysosome formation is important for cell-autonomous immunity to intracellular pathogens, antigen presentation and metabolism. A hallmark feature of phago-lysosomal compartments is that they undergo progressive luminal acidification controlled by the activation of vacuolar V-ATPase. Acidification is required for many enzymatic processes taking place in phago-lysosomes, like proteolysis, and supports the microbicidal activity of macrophages. Here we present a new quantitative methodology to assess phagosome acidification by flow cytometry based on the use of bi-fluorescent particles. This method relies on the use of UV polystyrene beads labelled with the acid sensor pHrodo-succinimidyl ester (pHrodo(TM) SE red) and enables us to dissociate particle association with phagocytes from their engulfment in acidified compartments. This methodology is well suited to monitor the acidification of phagosomes formed in vivo after fluorescent bead administration.

  10. Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry.

    PubMed

    Sica, Valentina; Maiuri, M Chiara; Kroemer, Guido; Galluzzi, Lorenzo

    2016-01-01

    Different modes of regulated cell death (RCD) can be initiated by distinct molecular machineries and their morphological manifestations can be difficult to discriminate. Moreover, cells responding to stress often activate an adaptive response centered around autophagy, and whether such a response is cytoprotective or cytotoxic cannot be predicted based on morphological parameters only. Molecular definitions are therefore important to understand various RCD subroutines from a mechanistic perspective. In vitro, various forms of RCD including apoptosis and autophagic cell death can be easily discriminated from each other with assays that involve chemical or pharmacological interventions targeting key components of either pathway. Here, we detail a straightforward method to discriminate apoptosis from autophagic cell death by flow cytometry, based on the broad-spectrum caspase inhibitor Z-VAD-fmk and the genetic inhibition of ATG5.

  11. Planning for ambulatory care: simple methods for improving patient flow.

    PubMed

    Schuh, S E; Tolins, I; Westphal, M C; Miller, M C

    1977-06-01

    A combined patient flow and work sampling study was done at the Ambulatory Pediatric Service of the Medical University of South Carolina. The biggest problem was that almost two thirds of the patient's time was spent waiting to see the doctor. Reasons for delay included too few examining rooms, the single block appointment system, and design of the facility.

  12. A diameter-sensitive flow entropy method for reliability consideration in water distribution system design

    NASA Astrophysics Data System (ADS)

    Liu, Haixing; Savić, Dragan; Kapelan, Zoran; Zhao, Ming; Yuan, Yixing; Zhao, Hongbin

    2014-07-01

    Flow entropy is a measure of uniformity of pipe flows in water distribution systems. By maximizing flow entropy one can identify reliable layouts or connectivity in networks. In order to overcome the disadvantage of the common definition of flow entropy that does not consider the impact of pipe diameter on reliability, an extended definition of flow entropy, termed as diameter-sensitive flow entropy, is proposed. This new methodology is then assessed by using other reliability methods, including Monte Carlo Simulation, a pipe failure probability model, and a surrogate measure (resilience index) integrated with water demand and pipe failure uncertainty. The reliability assessment is based on a sample of WDS designs derived from an optimization process for each of the two benchmark networks. Correlation analysis is used to evaluate quantitatively the relationship between entropy and reliability. To ensure reliability, a comparative analysis between the flow entropy and the new method is conducted. The results demonstrate that the diameter-sensitive flow entropy shows consistently much stronger correlation with the three reliability measures than simple flow entropy. Therefore, the new flow entropy method can be taken as a better surrogate measure for reliability and could be potentially integrated into the optimal design problem of WDSs. Sensitivity analysis results show that the velocity parameters used in the new flow entropy has no significant impact on the relationship between diameter-sensitive flow entropy and reliability.

  13. An empirical method for estimating travel times for wet volcanic mass flows

    USGS Publications Warehouse

    Pierson, Thomas C.

    1998-01-01

    Travel times for wet volcanic mass flows (debris avalanches and lahars) can be forecast as a function of distance from source when the approximate flow rate (peak discharge near the source) can be estimated beforehand. The near-source flow rate is primarily a function of initial flow volume, which should be possible to estimate to an order of magnitude on the basis of geologic, geomorphic, and hydrologic factors at a particular volcano. Least-squares best fits to plots of flow-front travel time as a function of distance from source provide predictive second-degree polynomial equations with high coefficients of determination for four broad size classes of flow based on near-source flow rate: extremely large flows (>1 000 000 m3/s), very large flows (10 000–1 000 000 m3/s), large flows (1000–10 000 m3/s), and moderate flows (100–1000 m3/s). A strong nonlinear correlation that exists between initial total flow volume and flow rate for "instantaneously" generated debris flows can be used to estimate near-source flow rates in advance. Differences in geomorphic controlling factors among different flows in the data sets have relatively little effect on the strong nonlinear correlations between travel time and distance from source. Differences in flow type may be important, especially for extremely large flows, but this could not be evaluated here. At a given distance away from a volcano, travel times can vary by approximately an order of magnitude depending on flow rate. The method can provide emergency-management officials a means for estimating time windows for evacuation of communities located in hazard zones downstream from potentially hazardous volcanoes.

  14. A new method for quantification of regurgitant flow rate using color Doppler flow imaging of the flow convergence region proximal to a discrete orifice. An in vitro study.

    PubMed

    Recusani, F; Bargiggia, G S; Yoganathan, A P; Raisaro, A; Valdes-Cruz, L M; Sung, H W; Bertucci, C; Gallati, M; Moises, V A; Simpson, I A

    1991-02-01

    While color Doppler flow mapping has yielded a quick and relatively sensitive method for visualizing the turbulent jets generated in valvular insufficiency, quantification of the degree of valvular insufficiency has been limited by the dependence of visualization of turbulent jets on hemodynamic as well as instrument-related factors. Color Doppler flow imaging, however, does have the capability of reliably showing the spatial relations of laminar flows. An area where flow accelerates proximal to a regurgitant orifice is commonly visualized on the left ventricular side of a mitral regurgitant orifice, especially when imaging is performed with high gain and a low pulse repetition frequency. This area of flow convergence, where the flow stream narrows symmetrically, can be quantified because velocity and the flow cross-sectional area change in inverse proportion along streamlines centered at the orifice. In this study, a gravity-driven constant-flow system with five sharp-edged diaphragm orifices (ranging from 2.9 to 12 mm in diameter) was imaged both parallel and perpendicular to the direction of flow through the orifice. Color Doppler flow images were produced by zero shifting so that the abrupt change in display color occurred at different velocities. This "aliasing boundary" with a known velocity and a measurable radial distance from the center of the orifice was used to determine an isovelocity hemisphere such that flow rate through the orifice was calculated as 2 pi r2 x Vr, where r is the radial distance from the center of the orifice to the color change and Vr is the velocity at which the color change was noted. Using Vr values from 54 to 14 cm/sec obtained with a 3.75-MHz transducer and from 75 to 18 cm/sec obtained with a 2.5-MHz transducer, we calculated flow rates and found them to correlate with measured flow rates (r = 0.94-0.99). The slope of the regression line was closest to unity when the lowest Vr and the correspondingly largest r were used in the

  15. Uncertainty Propagation for Turbulent, Compressible Flow in a Quasi-1D Nozzle Using Stochastic Methods

    NASA Technical Reports Server (NTRS)

    Zang, Thomas A.; Mathelin, Lionel; Hussaini, M. Yousuff; Bataille, Francoise

    2003-01-01

    This paper describes a fully spectral, Polynomial Chaos method for the propagation of uncertainty in numerical simulations of compressible, turbulent flow, as well as a novel stochastic collocation algorithm for the same application. The stochastic collocation method is key to the efficient use of stochastic methods on problems with complex nonlinearities, such as those associated with the turbulence model equations in compressible flow and for CFD schemes requiring solution of a Riemann problem. Both methods are applied to compressible flow in a quasi-one-dimensional nozzle. The stochastic collocation method is roughly an order of magnitude faster than the fully Galerkin Polynomial Chaos method on the inviscid problem.

  16. Comparison of Peak-Flow Estimation Methods for Small Drainage Basins in Maine

    USGS Publications Warehouse

    Hodgkins, Glenn A.; Hebson, Charles; Lombard, Pamela J.; Mann, Alexander

    2007-01-01

    Understanding the accuracy of commonly used methods for estimating peak streamflows is important because the designs of bridges, culverts, and other river structures are based on these flows. Different methods for estimating peak streamflows were analyzed for small drainage basins in Maine. For the smallest basins, with drainage areas of 0.2 to 1.0 square mile, nine peak streamflows from actual rainfall events at four crest-stage gaging stations were modeled by the Rational Method and the Natural Resource Conservation Service TR-20 method and compared to observed peak flows. The Rational Method had a root mean square error (RMSE) of -69.7 to 230 percent (which means that approximately two thirds of the modeled flows were within -69.7 to 230 percent of the observed flows). The TR-20 method had an RMSE of -98.0 to 5,010 percent. Both the Rational Method and TR-20 underestimated the observed flows in most cases. For small basins, with drainage areas of 1.0 to 10 square miles, modeled peak flows were compared to observed statistical peak flows with return periods of 2, 50, and 100 years for 17 streams in Maine and adjoining parts of New Hampshire. Peak flows were modeled by the Rational Method, the Natural Resources Conservation Service TR-20 method, U.S. Geological Survey regression equations, and the Probabilistic Rational Method. The regression equations were the most accurate method of computing peak flows in Maine for streams with drainage areas of 1.0 to 10 square miles with an RMSE of -34.3 to 52.2 percent for 50-year peak flows. The Probabilistic Rational Method was the next most accurate method (-38.5 to 62.6 percent). The Rational Method (-56.1 to 128 percent) and particularly the TR-20 method (-76.4 to 323 percent) had much larger errors. Both the TR-20 and regression methods had similar numbers of underpredictions and overpredictions. The Rational Method overpredicted most peak flows and the Probabilistic Rational Method tended to overpredict peak flows

  17. Comparing two methods of simulating mirco-scale viscous flows in a porous channel

    NASA Astrophysics Data System (ADS)

    Gao, Hui; Han, Jie; Jin, Yan; Wang, Lian-Ping

    2007-11-01

    Water flows in natural soil porous media are important to colloid-facilitated transport of contaminants and other phenomena with groundwater as the carrier. The 3D micro-scale flow is complicated due to the complex geometry. The transport and deposition of colloids in such flows are affected by several physical and chemical forces involved. In this talk, we first compare two methods of simulating viscous flows in both 2D and 3D channels filled with glass-bead particles. The first method is Physalis developed by Prosperetti's group, at Johns Hopkins, based on solving the Navier-Stokes equation using a combination of numerical solution and local analytical Stokes flow representation. The second method is a meso-scale approach by solving a lattice Boltzmann equation. Specific implementation issues will be discussed. The two methods yield almost identical flows. Preliminary simulation results as well as parallel experimental results on colloid deposition in the porous channel will also be presented.

  18. System and method for bidirectional flow and controlling fluid flow in a conduit

    DOEpatents

    Ortiz, M.G.

    1999-03-23

    A system for measuring bidirectional flow, including backflow, of fluid in a conduit is disclosed. The system utilizes a structural mechanism to create a pressure differential in the conduit. Pressure sensors are positioned upstream from the mechanism, at the mechanism, and downstream from the mechanism. Data from the pressure sensors are transmitted to a microprocessor or computer, and pressure differential detected between the pressure sensors is then used to calculate the backflow. Control signals may then be generated by the microprocessor or computer to shut off valves located in the conduit, upon the occurrence of backflow, or to control flow, total material dispersed, etc. in the conduit. 3 figs.

  19. System and method for bidirectional flow and controlling fluid flow in a conduit

    DOEpatents

    Ortiz, Marcos German

    1999-01-01

    A system for measuring bidirectional flow, including backflow, of fluid in a conduit. The system utilizes a structural mechanism to create a pressure differential in the conduit. Pressure sensors are positioned upstream from the mechanism, at the mechanism, and downstream from the mechanism. Data from the pressure sensors are transmitted to a microprocessor or computer, and pressure differential detected between the pressure sensors is then used to calculate the backflow. Control signals may then be generated by the microprocessor or computer to shut off valves located in the conduit, upon the occurrence of backflow, or to control flow, total material dispersed, etc. in the conduit.

  20. A theoretical method for the analysis and design of axisymmetric bodies. [flow distribution and incompressible fluids

    NASA Technical Reports Server (NTRS)

    Beatty, T. D.

    1975-01-01

    A theoretical method is presented for the computation of the flow field about an axisymmetric body operating in a viscous, incompressible fluid. A potential flow method was used to determine the inviscid flow field and to yield the boundary conditions for the boundary layer solutions. Boundary layer effects in the forces of displacement thickness and empirically modeled separation streamlines are accounted for in subsequent potential flow solutions. This procedure is repeated until the solutions converge. An empirical method was used to determine base drag allowing configuration drag to be computed.

  1. Flow processes in overexpanded chemical rocket nozzles. Part 3: Methods for the aimed flow separation and side load reduction

    NASA Technical Reports Server (NTRS)

    Schmucker, R. H.

    1983-01-01

    Methods aimed at reduction of overexpansion and side load resulting from asymmetric flow separation for rocket nozzles with a high opening ratio are described. The methods employ additional measures for nozzles with a fixed opening ratio. The flow separation can be controlled by several types of nozzle inserts, the properties of which are discussed. Side loads and overexpansion can be reduced by adapting the shape of the nozzle and taking other additional measures for controlled separation of the boundary layer, such as trip wires.

  2. Methods Used in Game Development to Foster FLOW

    NASA Technical Reports Server (NTRS)

    Jeppsen, Isaac Ben

    2010-01-01

    Games designed for entertainment have a rich history of providing compelling experiences. From consoles to PCs, games have managed to present intuitive and effective interfaces for a wide range of game styles to successfully allow users to "walk-up-and-play". Once a user is hooked, successful games artfully present challenging experiences just within reach of a user's ability, weaving each task and achievement into a compelling and engaging experience. In this paper, engagement is discussed in terms of the psychological theory of Flow. I argue that engagement should be one of the primary goals when developing a serious game and I discuss the best practices and techniques that have emerged from traditional video game development which help foster the creation of engaging, high Flow experiences.

  3. [Numerical flow simulation : A new method for assessing nasal breathing].

    PubMed

    Hildebrandt, T; Osman, J; Goubergrits, L

    2016-08-01

    The current options for objective assessment of nasal breathing are limited. The maximum they can determine is the total nasal resistance. Possibilities to analyze the endonasal airstream are lacking. In contrast, numerical flow simulation is able to provide detailed information of the flow field within the nasal cavity. Thus, it has the potential to analyze the nasal airstream of an individual patient in a comprehensive manner and only a computed tomography (CT) scan of the paranasal sinuses is required. The clinical application is still limited due to the necessary technical and personnel resources. In particular, a statistically based referential characterization of normal nasal breathing does not yet exist in order to be able to compare and classify the simulation results.

  4. Expiratory flow limitation definition, mechanisms, methods, and significance.

    PubMed

    Tantucci, Claudio

    2013-01-01

    When expiratory flow is maximal during tidal breathing and cannot be increased unless operative lung volumes move towards total lung capacity, tidal expiratory flow limitation (EFL) is said to occur. EFL represents a severe mechanical constraint caused by different mechanisms and observed in different conditions, but it is more relevant in terms of prevalence and negative consequences in obstructive lung diseases and particularly in chronic obstructive pulmonary disease (COPD). Although in COPD patients EFL more commonly develops during exercise, in more advanced disorder it can be present at rest, before in supine position, and then in seated-sitting position. In any circumstances EFL predisposes to pulmonary dynamic hyperinflation and its unfavorable effects such as increased elastic work of breathing, inspiratory muscles dysfunction, and progressive neuroventilatory dissociation, leading to reduced exercise tolerance, marked breathlessness during effort, and severe chronic dyspnea.

  5. Method of producing monolithic ceramic cross-flow filter

    DOEpatents

    Larsen, D.A.; Bacchi, D.P.; Connors, T.F.; Collins, E.L. III

    1998-02-10

    Ceramic filter of various configuration have been used to filter particulates from hot gases exhausted from coal-fired systems. Prior ceramic cross-flow filters have been favored over other types, but those previously have been assemblies of parts somehow fastened together and consequently subject often to distortion or delamination on exposure hot gas in normal use. The present new monolithic, seamless, cross-flow ceramic filters, being of one-piece construction, are not prone to such failure. Further, these new products are made by a novel casting process which involves the key steps of demolding the ceramic filter green body so that none of the fragile inner walls of the filter is cracked or broken. 2 figs.

  6. Method of producing monolithic ceramic cross-flow filter

    DOEpatents

    Larsen, David A.; Bacchi, David P.; Connors, Timothy F.; Collins, III, Edwin L.

    1998-01-01

    Ceramic filter of various configuration have been used to filter particulates from hot gases exhausted from coal-fired systems. Prior ceramic cross-flow filters have been favored over other types, but those previously horn have been assemblies of parts somehow fastened together and consequently subject often to distortion or delamination on exposure hot gas in normal use. The present new monolithic, seamless, cross-flow ceramic filters, being of one-piece construction, are not prone to such failure. Further, these new products are made by novel casting process which involves the key steps of demolding the ceramic filter green body so that none of the fragile inner walls of the filter is cracked or broken.

  7. System and method measuring fluid flow in a conduit

    DOEpatents

    Ortiz, M.G.; Kidd, T.G.

    1999-05-18

    A system is described for measuring fluid mass flow in a conduit in which there exists a pressure differential in the fluid between at least two spaced-apart locations in the conduit. The system includes a first pressure transducer disposed in the side of the conduit at a first location for measuring pressure of fluid at that location, a second or more pressure transducers disposed in the side of the conduit at a second location, for making multiple measurements of pressure of fluid in the conduit at that location, and a computer for computing the average pressure of the multiple measurements at the second location and for computing flow rate of fluid in the conduit from the pressure measurement by the first pressure transducer and from the average pressure calculation of the multiple measurements. 3 figs.

  8. System and method measuring fluid flow in a conduit

    DOEpatents

    Ortiz, Marcos German; Kidd, Terrel G.

    1999-01-01

    A system for measuring fluid mass flow in a conduit in which there exists a pressure differential in the fluid between at least two spaced-apart locations in the conduit. The system includes a first pressure transducer disposed in the side of the conduit at a first location for measuring pressure of fluid at that location, a second or more pressure transducers disposed in the side of the conduit at a second location, for making multiple measurements of pressure of fluid in the conduit at that location, and a computer for computing the average pressure of the multiple measurements at the second location and for computing flow rate of fluid in the conduit from the pressure measurement by the first pressure transducer and from the average pressure calculation of the multiple measurements.

  9. A Muskingum-Cunge Channel Flow Routing Method for Drainage Networks

    DTIC Science & Technology

    1991-11-01

    A AD-A247 020 US Army Corps of Engineers Hydrologic Engineering Center A Muskingum-Cunge Channel Flow Routing Method for Drainage Networks DTIC...Flow Routing Method for Drainage Networks November 1991 By Jurgen Garbrecht National Agricultural Water Quality Laboratory USDA - Agricultural...CA 95616-4687 (916) 756-1104 TP-135 2 SUMMARY A Muskingum-Cunge channel flow routing scheme is modified for application to large drainage networks with

  10. Turbulence Modeling for Thrust Reverser Flow Field Prediction Methods

    DTIC Science & Technology

    1992-12-01

    Barata 28 of a normal impinging jet at H/D = 5 and Vj/V_. = 30 indicate that the shear stress in the vortex is roughly an order of magnitude less than...Speed Afterbody Flows," 1. Propulsion and Power, Vol. 7, No. 4, 1991, pp. 607-616 28. Barata , J. M. M., Durao, D. F. G., and Heitor, M. V., "Turbulent

  11. Spectral element methods for transitional flows, in complex geometries.

    SciTech Connect

    Fischer, P. F.; Kruse, G. W.; Loth, F.; Mathematics and Computer Science; Juniata Coll.; Univ. of Illinois

    2002-01-01

    We describe the development and implementation of an efficient spectral element code for simulating transitional flows in complex three-dimensional domains. Critical to this effort is the use of geometrically nonconforming elements that allow localized refinement in regions of interest, coupled with a stabilized high-order time-split formulation of the semi-discrete Navier-Stokes equations. Simulations of transition in a model of an arteriovenous graft illustrate the potential of this approach in biomechanical applications.

  12. Adaptive finite element methods for compressible flow problems

    NASA Technical Reports Server (NTRS)

    Oden, J. T.; Strouboulis, T.; Devloo, PH.

    1986-01-01

    Some recent work on adaptive FEMs for solving transient Euler equations in two-dimensional domains is summarized. The formulation of an FEM model of the Euler equations is shown, and the application of the adaptive strategies to data management schemes is addressed. Sample numerical results from the application of the model and strategies to the flow over a step and to transient cases are given.

  13. Basic numerical methods. [of unsteady and transonic flow

    NASA Technical Reports Server (NTRS)

    Steger, Joseph L.; Van Dalsem, William R.

    1989-01-01

    Some of the basic finite-difference schemes that can be used to solve the nonlinear equations that describe unsteady inviscid and viscous transonic flow are reviewed. Numerical schemes for solving the unsteady Euler and Navier-Stokes, boundary-layer, and nonlinear potential equations are described. Emphasis is given to the elementary ideas used in constructing various numerical procedures, not specific details of any one procedure.

  14. Improved numerical methods for turbulent viscous recirculating flows

    NASA Technical Reports Server (NTRS)

    Turan, A.

    1985-01-01

    The hybrid-upwind finite difference schemes employed in generally available combustor codes possess excessive numerical diffusion errors which preclude accurate quantative calculations. The present study has as its primary objective the identification and assessment of an improved solution algorithm as well as discretization schemes applicable to analysis of turbulent viscous recirculating flows. The assessment is carried out primarily in two dimensional/axisymetric geometries with a view to identifying an appropriate technique to be incorporated in a three-dimensional code.

  15. Improved numerical methods for turbulent viscous recirculating flows

    NASA Technical Reports Server (NTRS)

    Vandoormaal, J. P.; Turan, A.; Raithby, G. D.

    1986-01-01

    The objective of the present study is to improve both the accuracy and computational efficiency of existing numerical techniques used to predict viscous recirculating flows in combustors. A review of the status of the study is presented along with some illustrative results. The effort to improve the numerical techniques consists of the following technical tasks: (1) selection of numerical techniques to be evaluated; (2) two dimensional evaluation of selected techniques; and (3) three dimensional evaluation of technique(s) recommended in Task 2.

  16. An unconditionally stable Runge-Kutta method for unsteady flows

    NASA Technical Reports Server (NTRS)

    Jorgenson, Philip C. E.; Chima, Rodrick V.

    1989-01-01

    A quasi-three-dimensional analysis was developed for unsteady rotor-stator interaction in turbomachinery. The analysis solves the unsteady Euler or thin-layer Navier-Stokes equations in a body-fitted coordinate system. It accounts for the effects of rotation, radius change, and stream surface thickness. The Baldwin-Lomax eddy viscosity model is used for turbulent flows. The equations are integrated in time using a four-stage Runge-Kutta scheme with a constant time step. Implicit residual smoothing was employed to accelerate the solution of the time accurate computations. The scheme is described and accuracy analyses are given. Results are shown for a supersonic through-flow fan designed for NASA Lewis. The rotor:stator blade ratio was taken as 1:1. Results are also shown for the first stage of the Space Shuttle Main Engine high pressure fuel turbopump. Here the blade ratio is 2:3. Implicit residual smoothing was used to increase the time step limit of the unsmoothed scheme by a factor of six with negligible differences in the unsteady results. It is felt that the implicitly smoothed Runge-Kutta scheme is easily competitive with implicit schemes for unsteady flows while retaining the simplicity of an explicit scheme.

  17. An unconditionally stable Runge-Kutta method for unsteady flows

    NASA Technical Reports Server (NTRS)

    Jorgenson, Philip C. E.; Chima, Rodrick V.

    1988-01-01

    A quasi-three dimensional analysis was developed for unsteady rotor-stator interaction in turbomachinery. The analysis solves the unsteady Euler or thin-layer Navier-Stokes equations in a body fitted coordinate system. It accounts for the effects of rotation, radius change, and stream surface thickness. The Baldwin-Lomax eddy viscosity model is used for turbulent flows. The equations are integrated in time using a four stage Runge-Kutta scheme with a constant time step. Implicit residual smoothing was employed to accelerate the solution of the time accurate computations. The scheme is described and accuracy analyses are given. Results are shown for a supersonic through-flow fan designed for NASA Lewis. The rotor:stator blade ratio was taken as 1:1. Results are also shown for the first stage of the Space Shuttle Main Engine high pressure fuel turbopump. Here the blade ratio is 2:3. Implicit residual smoothing was used to increase the time step limit of the unsmoothed scheme by a factor of six with negligible differences in the unsteady results. It is felt that the implicitly smoothed Runge-Kutta scheme is easily competitive with implicit schemes for unsteady flows while retaining the simplicity of an explicit scheme.

  18. New method for detecting singularities in experimental incompressible flows

    NASA Astrophysics Data System (ADS)

    Kuzzay, Denis; Saw, Ewe-Wei; Martins, Fabio J. W. A.; Faranda, Davide; Foucaut, Jean-Marc; Daviaud, François; Dubrulle, Bérengère

    2017-06-01

    We introduce two new criteria based on the work of Duchon and Robert (2000 Nonlinearity 13 249) and Eyink (2006 Phys. Rev. E 74 066302), which allow for the local detection of Navier-Stokes singularities in experimental flows. We discuss the difference between non-dissipative or dissipative Euler quasi-singularities and genuine Navier-Stokes dissipative singularites, and classify them with respect to their Hölder exponent h. We show that our criteria allow us to detect areas in a flow where the velocity field is no more regular than Hölder continuous with some Hölder exponent h ≤slant 1/2 . We illustrate our discussion using classical tomographic particle image velocimetry (TPIV) measurements obtained inside a high Reynolds number flow generated in the boundary layer of a wind tunnel. Our study shows that, in order to detect singularities or quasi-singularities, one does not need to have access to the whole velocity field inside a volume, but can instead look for them from stereoscopic PIV data on a plane. We also provide a discussion about the link between areas detected by our criteria and areas corresponding to large vorticity. We argue that this link might provide either a clue about the genesis of these quasi-singularities or a way to discriminate dissipative Euler quasi-singularities and genuine Navier-Stokes singularities.

  19. Flow instability of a centrifugal pump determined using the energy gradient method

    NASA Astrophysics Data System (ADS)

    Li, Yi; Dong, Wenlong; He, Zhaohui; Huang, Yuanmin; Jiang, Xiaojun

    2015-02-01

    The stability of the centrifugal pump has not been well revealed because of the complexity of internal flow. To analyze the flow characteristics of a centrifugal pump operating at low capacity, methods of numerical simulation and experimental research were adopted in this paper. Characteristics of the inner flow were obtained. Standard k-ɛ turbulence models were used to calculate the inner flow of the pump under off-design conditions. The distribution of the energy gradient function K was obtained by three-dimensional numerical simulation at different flow rates. The relative velocity component was acquired from the absolute velocity obtained in particle image velocimetry. By comparing with experimental results, it was found that flow instability occurs at the position of maximum K. The flow stability reduces with an increasing flow rate. The research results provide a theoretical basis for the optimization design of a centrifugal pump.

  20. A potential-flow/boundary-layer method for calculating subsonic and transonic airfoil flow with trailing-edge separation

    NASA Technical Reports Server (NTRS)

    Barnwell, R. W.

    1981-01-01

    The development of a potential-flow/boundary-layer method for calculating subsonic and transonic turbulent flow past airfoils with trailing-edge separation is reported. A moment-of-momentum integral boundary-layer method is used which employs the law-of-the-wall/law-of-the-wake velocity profile and a two-layer eddy-viscosity model and ignores the laminar sublayer. All integrals across the boundary layer are obtained in closed form. Separation is assumed to occur when the shearing-stress velocity vanishes. A closed-form solution is derived for separated-flow regions where the shearing stress is negligible. In the potential-flow method, the exact form of the airfoil boundary condition is used, but it is applied at the chord line rather than the airfoil surface. This allows the accurate computation of flow about airfoils at large angles of attack but permits the use of body-oriented Cartesian computational grids. The governing equation for the perturbation velocity potential contains several terms in addition to the classical small-disturbance terms.

  1. A robust method for estimating respiratory flow using tracheal sounds entropy.

    PubMed

    Yadollahi, Azadeh; Moussavi, Zahra M K

    2006-04-01

    The relationship between respiratory sounds and flow is of great interest for researchers and physicians due to its diagnostic potentials. Due to difficulties and inaccuracy of most of the flow measurement techniques, several researchers have attempted to estimate flow from respiratory sounds. However, all of the proposed methods heavily depend on the availability of different rates of flow for calibrating the model, which makes their use limited by a large degree. In this paper, a robust and novel method for estimating flow using entropy of the band pass filtered tracheal sounds is proposed. The proposed method is novel in terms of being independent of the flow rate chosen for calibration; it requires only one breath for calibration and can estimate any flow rate even out of the range of calibration flow. After removing the effects of heart sounds (which distort the low-frequency components of tracheal sounds) on the calculated entropy of the tracheal sounds, the performance of the method at different frequency ranges were investigated. Also, the performance of the proposed method was tested using 6 different segment sizes for entropy calculation and the best segment sizes during inspiration and expiration were found. The method was tested on data of 10 healthy subjects at five different flow rates. The overall estimation error was found to be 8.3 +/- 2.8% and 9.6 +/- 2.8% for inspiration and expiration phases, respectively.

  2. Tracking and Measurement of the Motion of Blood Cells Using Optical Flow Methods

    PubMed Central

    Guo, Dongmin; Van de Ven, Anne L.; Zhou, Xiaobo

    2014-01-01

    The investigation of microcirculation is a critical task in biomedical and physiological research. In order to monitor human’s condition and develop effective therapies of some diseases, the microcirculation information, such as flow velocity and vessel density, must be evaluated in a noninvasive manner. As one of the tasks of microcirculation investigation, automatic blood cell tracking presents an effective approach to estimate blood flow velocity. Currently, the most common method for blood cell tracking is based on spatiotemporal image analysis, which has lots of limitations, such as the diameter of microvesssels cannot be too larger than blood cells or tracers, cells or tracers should have fixed velocity, and it requires the image with high qualification. In this paper, we propose an optical flow method for automatic cell tracking. The key algorithm of the method is to align an image to its neighbors in a large image collection consisting of a variety of scenes. Considering the method cannot solve the problems in all cases of cell movement, another optical flow method, SIFT (Scale Invariant Feature Transform) flow, is also presented. The experimental results show that both methods can track the cells accurately. Optical flow is specially robust to the case where the velocity of cell is unstable, while SIFT flow works well when there are large displacement of cell between two adjacent frames. Our proposed methods outperform other methods when doing in vivo cell tracking, which can be used to estimate the blood flow directly and help to evaluate other parameters in microcirculation. PMID:24058034

  3. A Method to Evaluate Groundwater flow system under the Seabed

    NASA Astrophysics Data System (ADS)

    Kohara, N.; Marui, A.

    2011-12-01

    / fresh water interface (position of the submarine groundwater discharge) may appear on the seafloor. Moreover, neither the salinity concentration nor the groundwater age depends on depth. It is thought that it is because that the groundwater forms the complex flow situation through the change in a long-term groundwater flow system. The technology to understand the coastal groundwater flow consists of remote sensing, geographical features analysis, surface of the earth investigation, geophysical exploration, drilling survey, and indoor examination and the measurement. Integration of each technology is needed to interpret groundwater flow system because the one is to catch the local groundwater flow in the time series and another one is to catch the long-term and regional groundwater flow in the general situation. The purpose of this study is to review the previous research of coastal groundwater flow, and to integrate an applicable evaluation approach to understand this mechanism. In this presentation, the review of the research and case study using numerical simulation are introduced.

  4. The numerical method of calculation of two-dimensional stationary electro-gasdynamic internal flows

    NASA Astrophysics Data System (ADS)

    Zhurin, V. V.; Shipilin, A. V.

    The suggested numerical method is meant for computations of two dimensional stationary internal nonviscous gas flows in a presence of a heat supply at expense of Joule heating. Such flows and processes take place in the plasma thrusters and in the working regions of the electro-ionizing lasers. The numerical solution of the Euler equations for subsonic and transonic regimes are considered. For computations of supersonic internal flows the method of characteristics is a very effective method. The method suggested allows an easy connection with the method of characteristics.

  5. Method and system for measuring multiphase flow using multiple pressure differentials

    DOEpatents

    Fincke, James R.

    2001-01-01

    An improved method and system for measuring a multiphase flow in a pressure flow meter. An extended throat venturi is used and pressure of the multiphase flow is measured at three or more positions in the venturi, which define two or more pressure differentials in the flow conduit. The differential pressures are then used to calculate the mass flow of the gas phase, the total mass flow, and the liquid phase. The method for determining the mass flow of the high void fraction fluid flow and the gas flow includes certain steps. The first step is calculating a gas density for the gas flow. The next two steps are finding a normalized gas mass flow rate through the venturi and computing a gas mass flow rate. The following step is estimating the gas velocity in the venturi tube throat. The next step is calculating the pressure drop experienced by the gas-phase due to work performed by the gas phase in accelerating the liquid phase between the upstream pressure measuring point and the pressure measuring point in the venturi throat. Another step is estimating the liquid velocity in the venturi throat using the calculated pressure drop experienced by the gas-phase due to work performed by the gas phase. Then the friction is computed between the liquid phase and a wall in the venturi tube. Finally, the total mass flow rate based on measured pressure in the venturi throat is calculated, and the mass flow rate of the liquid phase is calculated from the difference of the total mass flow rate and the gas mass flow rate.

  6. Gas-Liquid Two-Phase Flow Distribution Using Phase Separation Method

    NASA Astrophysics Data System (ADS)

    Zhang, B. D.; Liu, D.; Wang, D.

    2010-03-01

    A method for gas-liquid two-phase flow distribution is proposed in this study, which can be called the phase separation method. The advantage of the new method is that it converts two-phase flow distribution into single-phase distribution, which overcomes the problem of phase splitting in the distribution process of two-phase flow radically, and an equal quality distribution is guaranteed. At first, separate the mixture of gas and liquid into single or near single phase fluids by enhancing phase splitting in distributor, then distribute the single gas and liquid flow respectively as required, finally recombine each couple of gas and liquid stream respectively to form a two phase stream exiting a branch. Experiments were conducted in an air-water multiphase flow test loop. The flow pattern in the experiments included stratified flow, wave flow, slug flow and a part of annular flow. The experimental results show that the phase separation method and apparatus could be feasible to make an equal quality distribution and the deviation of stream quality among the branches is less than 1.6%.

  7. Method of characteristics for three-dimensional axially symmetrical supersonic flows.

    NASA Technical Reports Server (NTRS)

    Sauer, R

    1947-01-01

    An approximation method for three-dimensional axially symmetrical supersonic flows is developed; it is based on the characteristics theory (represented partly graphically, partly analytically). Thereafter this method is applied to the construction of rotationally symmetrical nozzles. (author)

  8. Method and apparatus to measure vapor pressure in a flow system

    DOEpatents

    Grossman, Mark W.; Biblarz, Oscar

    1991-01-01

    The present invention is directed to a method for determining, by a condensation method, the vapor pressure of a material with a known vapor pressure versus temperature characteristic, in a flow system particularly in a mercury isotope enrichment process.

  9. Kinetic theory based new upwind methods for inviscid compressible flows

    NASA Technical Reports Server (NTRS)

    Deshpande, S. M.

    1986-01-01

    Two new upwind methods called the Kinetic Numerical Method (KNM) and the Kinetic Flux Vector Splitting (KFVS) method for the solution of the Euler equations have been presented. Both of these methods can be regarded as some suitable moments of an upwind scheme for the solution of the Boltzmann equation provided the distribution function is Maxwellian. This moment-method strategy leads to a unification of the Riemann approach and the pseudo-particle approach used earlier in the development of upwind methods for the Euler equations. A very important aspect of the moment-method strategy is that the new upwind methods satisfy the entropy condition because of the Boltzmann H-Theorem and suggest a possible way of extending the Total Variation Diminishing (TVD) principle within the framework of the H-Theorem. The ability of these methods in obtaining accurate wiggle-free solution is demonstrated by applying them to two test problems.

  10. Comparison of wind tunnel test results at free stream Mach 0.7 with results from the Boeing TEA-230 subsonic flow method. [wing flow method tests

    NASA Technical Reports Server (NTRS)

    Mohn, L. W.

    1975-01-01

    The use of the Boeing TEA-230 Subsonic Flow Analysis method as a primary design tool in the development of cruise overwing nacelle configurations is presented. Surface pressure characteristics at 0.7 Mach number were determined by the TEA-230 method for a selected overwing flow-through nacelle configuration. Results of this analysis show excellent overall agreement with corresponding wind tunnel data. Effects of the presence of the nacelle on the wing pressure field were predicted accurately by the theoretical method. Evidence is provided that differences between theoretical and experimental pressure distributions in the present study would not result in significant discrepancies in the nacelle lines or nacelle drag estimates.

  11. PHYSALIS: a new method for particle flow simulation. Part III: convergence analysis of two-dimensional flows

    NASA Astrophysics Data System (ADS)

    Huang, Huaxiong; Takagi, Shu

    2003-08-01

    In this paper, we study the convergence property of PHYSALIS when it is applied to incompressible particle flows in two-dimensional space. PHYSALIS is a recently proposed iterative method which computes the solution without imposing the boundary conditions on the particle surfaces directly. Instead, a consistency equation based on the local (near particle) representation of the solution is used as the boundary conditions. One of the important issues needs to be addressed is the convergence properties of the iterative procedure. In this paper, we present the convergence analysis using Laplace and biharmonic equations as two model problems. It is shown that convergence of the method can be achieved but the rate of convergence depends on the relative locations of the cages. The results are directly related to potential and Stokes flows. However, they are also relevant to Navier-Stokes flows, heat conduction in composite media, and other problems.

  12. Solution of transonic flows by an integro-differential equation method

    NASA Technical Reports Server (NTRS)

    Ogana, W.

    1978-01-01

    Solutions of steady transonic flow past a two-dimensional airfoil are obtained from a singular integro-differential equation which involves a tangential derivative of the perturbation velocity potential. Subcritical flows are solved by taking central differences everywhere. For supercritical flows with shocks, central differences are taken in subsonic flow regions and backward differences in supersonic flow regions. The method is applied to a nonlifting parabolic-arc airfoil and to a lifting NACA 0012 airfoil. Results compare favorably with those of finite-difference schemes.

  13. Measuring Flow Rate in Crystalline Bedrock Wells Using the Dissolved Oxygen Alteration Method.

    PubMed

    Vitale, Sarah A; Robbins, Gary A

    2017-03-22

    Determination of vertical flow rates in a fractured bedrock well can aid in planning and implementing hydraulic tests, water quality sampling, and improving interpretations of water quality data. Although flowmeters are highly accurate in flow rate measurement, the high cost and logistics may be limiting. In this study the dissolved oxygen alteration method (DOAM) is expanded upon as a low-cost alternative to determine vertical flow rates in crystalline bedrock wells. The method entails altering the dissolved oxygen content in the wellbore through bubbler aeration, and monitoring the vertical advective movement of the dissolved oxygen over time. Measurements were taken for upward and downward flows, and under ambient and pumping conditions. Vertical flow rates from 0.06 to 2.30 Lpm were measured. To validate the method, flow rates determined with the DOAM were compared to pump discharge rates and found to be in agreement within 2.5%.

  14. Flow Rate Measurement Using {sup 99m}Tc Radiotracer Method in a Pipe Installation

    SciTech Connect

    Sipaun, S. M.; Bakar, A. Q. Abu; Othman, N.; Shaari, M. R.; Adnan, M. A. K.; Yusof, J. Mohd; Demanah, R.

    2010-07-07

    Flow rate is a significant parameter for managing processes in chemical processing plants and water processing facility. Accurate measurement of the flow rate allows engineers to monitor the delivery of process material, which in turn impacts a plant's capacity to produce their products. One of the available methods for determining the flow rate of a process material is by introducing a radiotracer to the system that mimics the material's flow pattern. In this study, a low activity Technetium-99m radioisotope was injected into a water piping setup and the 2'' x 2'' NaI (Tl) detectors were calibrated to detect spectrum peaks at specific points of the pipe installation. Using pulse velocity method, water flow rate was determined to be 11.3 litres per minute. For the sampling method, at different pump capacity, the flow rate was 15.0 litres per minute.

  15. Detection and isolation of rare cells by 2-step enrichment high-speed flow cytometry/cell sorting and single cell LEAP laser ablation

    NASA Astrophysics Data System (ADS)

    Zordan, M. D.; Leary, James F.

    2011-02-01

    The clonal isolation of rare cells, especially cancer and stem cells, in a population is important to the development of improved medical treatment. We have demonstrated that the Laser-Enabled Analysis and Processing (LEAP, Cyntellect Inc., San Diego, CA) instrument can be used to efficiently produce single cell clones by photoablative dilution. Additionally, we have also shown that cells present at low frequencies can be cloned by photoablative dilution after they are pre-enriched by flow cytometry based cell sorting. Circulating tumor cells were modeled by spiking isolated peripheral blood cells with cells from the lung carcinoma cell line A549. Flow cytometry based cell sorting was used to perform an enrichment sort of A549 cells directly into a 384 well plate. Photoablative dilution was performed with the LEAPTM instrument to remove any contaminating cells, and clonally isolate 1 side population cell per well. We were able to isolate and grow single clones of side population cells using this method at greater than 90% efficiency. We have developed a 2 step method that is able to perform the clonal isolation of rare cells based on a medically relevant functional phenotype.

  16. An Efficient Inverse Aerodynamic Design Method For Subsonic Flows

    NASA Technical Reports Server (NTRS)

    Milholen, William E., II

    2000-01-01

    Computational Fluid Dynamics based design methods are maturing to the point that they are beginning to be used in the aircraft design process. Many design methods however have demonstrated deficiencies in the leading edge region of airfoil sections. The objective of the present research is to develop an efficient inverse design method which is valid in the leading edge region. The new design method is a streamline curvature method, and a new technique is presented for modeling the variation of the streamline curvature normal to the surface. The new design method allows the surface coordinates to move normal to the surface, and has been incorporated into the Constrained Direct Iterative Surface Curvature (CDISC) design method. The accuracy and efficiency of the design method is demonstrated using both two-dimensional and three-dimensional design cases.

  17. Dissolution testing of acetylsalicylic acid by a channel flow method-correlation to USP basket and intrinsic dissolution methods.

    PubMed

    Peltonen, Leena; Liljeroth, Peter; Heikkilä, Tiina; Kontturi, Kyösti; Hirvonen, Jouni

    2003-08-01

    A new modification of the channel flow dissolution method is introduced together with the theoretical basis to extract the solubility and mass transfer parameters from the dissolution experiments. Correlation of drug dissolution profiles in the channel flow apparatus was evaluated with respect to USP basket and intrinsic dissolution methods at pH 1.2 or 6.8. Acetylsalicylic acid (ASA) was studied as a pure drug substance and as three simple tablet compositions with microcrystalline cellulose (MCC) and/or lactose as excipients. The channel flow measurements of 100% ASA tablets correlated well with the results of intrinsic dissolution tests. In the channel flow method as well as in the USP basket method the release of ASA was fastest from the tablet compositions containing lactose, while the slowest dissolution rate was observed with the composition containing MCC as the only excipient. As presumed, the dissolution rate of the weak acid was decreased as the pH of the medium was lowered, which was clearly confirmed also by the three dissolution methods. MCC forms matrix tablets and in the USP basket method the dissolution profiles followed square root of time kinetics indicating that diffusion was the rate-controlling step of ASA dissolution. Also the channel flow results indicated that the dissolution of ASA was controlled by mass transfer. The swelling behaviour of the tablets is different in the channel flow method as compared to the basket method: only one tablet surface is exposed to the dissolution medium in the channel flow system. The contact between the tablet surface and the dissolution medium is more similar between the channel flow and intrinsic dissolution methods.

  18. Locally Conservative, Stabilized Finite Element Methods for Variably Saturated Flow

    DTIC Science & Technology

    2007-11-06

    mixed methods for Richards’ equation. The effectiveness of the multiscale stabilization strategy varied somewhat. For a steady-state, variably...Arbogast, Z. Chen, On the implementation of mixed methods as non- conforming methods for second order elliptic problems, Mathematics of Computation 64...211) (1995) 943–972. [53] Z. Chen, Equivalence between and multigrid algorithms for nonconform- ing and mixed methods for second order elliptic

  19. Method and apparatus for chemically altering fluids in continuous flow

    DOEpatents

    Heath, William O.; Virden, Jr., Judson W.; Richardson, R. L.; Bergsman, Theresa M.

    1993-01-01

    The present invention relates to a continuous flow fluid reactor for chemically altering fluids. The reactor operates on standard frequency (50 to 60 Hz) electricity. The fluid reactor contains particles that are energized by the electricity to form a corona throughout the volume of the reactor and subsequently a non-equilibrium plasma that interacts with the fluid. Particles may form a fixed bed or a fluid bed. Electricity may be provided through electrodes or through an inductive coil. Fluids include gases containing exhaust products and organic fuels requiring oxidation.

  20. Method and apparatus for chemically altering fluids in continuous flow

    DOEpatents

    Heath, W.O.; Virden, J.W. Jr.; Richardson, R.L.; Bergsman, T.M.

    1993-10-19

    The present invention relates to a continuous flow fluid reactor for chemically altering fluids. The reactor operates on standard frequency (50 to 60 Hz) electricity. The fluid reactor contains particles that are energized by the electricity to form a corona throughout the volume of the reactor and subsequently a non-equilibrium plasma that interacts with the fluid. Particles may form a fixed bed or a fluid bed. Electricity may be provided through electrodes or through an inductive coil. Fluids include gases containing exhaust products and organic fuels requiring oxidation. 4 figures.

  1. Characterization of Vapor and Aerosol Flows by Photothermal Methods.

    DTIC Science & Technology

    2014-09-26

    transient Mie-scattering. The main parts of our experimental set-up have been shown previously5 . A Q-switched CO2 laser beam (pulse duration I ps...indicated beam separations in a nitrogen/ethanol vapor flow.The CO2 laser is fired at t-0. Signal decrease and broadening is in agreement with eq. (I...20. AUSTRACT (Confliu W tover@ aede It nemsaem iaind Identif by bleck numier) Pulsed laser heating is used to label aerosols or absorbing vapors

  2. Numerical Methods for Granular Flows and Related Problems

    DTIC Science & Technology

    2007-11-02

    of the resulting pile cannot exceed some critical value: the angle of repose . The pile takes a conical shape, such as for instance that of sand in the...around a circular cylindrical obstacle for a material with an angle of repose of δ = 40◦ 5. TECHNOLOGY TRANSFER. We will pursue our collaboration with...Fig. 4.2. Stream function showing secondary flow; top: tilted hopper (m = 1), bottom: ”elliptical” hopper (m = 2). Angle of wall friction = 15◦ (µ = tan

  3. Blood flow determination using recursive processing: a digital radiographic method

    SciTech Connect

    Kruger, R.A.; Bateman, W.; Liu, P.Y.; Nelson, J.A.

    1983-10-01

    Temporal filtration of fluoroscopic video sequences is being used as an alternative to pulsed digital subtraction angiography. Using the same image processing architecture and a slight modification in processing logic a parametric image can be synthesized from such a temporally filtered image sequence in virtual real time, i.e., an image sequence that spans T seconds takes exactly T seconds to process. Off-line computer processing is not required. Initial phantom studies imply that the time to maximum opacification (t/sub max/) can be used to determine absolute and relative blood flow with a high confidence level (r > .989). Phantom and animal examples are presented.

  4. Iterative spectral methods and spectral solutions to compressible flows

    NASA Technical Reports Server (NTRS)

    Hussaini, M. Y.; Zang, T. A.

    1982-01-01

    A spectral multigrid scheme is described which can solve pseudospectral discretizations of self-adjoint elliptic problems in O(N log N) operations. An iterative technique for efficiently implementing semi-implicit time-stepping for pseudospectral discretizations of Navier-Stokes equations is discussed. This approach can handle variable coefficient terms in an effective manner. Pseudospectral solutions of compressible flow problems are presented. These include one dimensional problems and two dimensional Euler solutions. Results are given both for shock-capturing approaches and for shock-fitting ones.

  5. Evaluation of a method of estimating low-flow frequencies from base-flow measurements at Indiana streams

    USGS Publications Warehouse

    Wilson, John Thomas

    2000-01-01

    A mathematical technique of estimating low-flow frequencies from base-flow measurements was evaluated by using data for streams in Indiana. Low-flow frequencies at low- flow partial-record stations were estimated by relating base-flow measurements to concurrent daily flows at nearby streamflow-gaging stations (index stations) for which low-flowfrequency curves had been developed. A network of long-term streamflow-gaging stations in Indiana provided a sample of sites with observed low-flow frequencies. Observed values of 7-day, 10-year low flow and 7-day, 2-year low flow were compared to predicted values to evaluate the accuracy of the method. Five test cases were used to evaluate the method under a variety of conditions in which the location of the index station and its drainage area varied relative to the partial-record station. A total of 141 pairs of streamflow-gaging stations were used in the five test cases. Four of the test cases used one index station, the fifth test case used two index stations. The number of base-flow measurements was varied for each test case to see if the accuracy of the method was affected by the number of measurements used. The most accurate and least variable results were produced when two index stations on the same stream or tributaries of the partial-record station were used. All but one value of the predicted 7-day, 10-year low flow were within 15 percent of the values observed for the long-term continuous record, and all of the predicted values of the 7-day, 2-year lowflow were within 15 percent of the observed values. This apparent accuracy, to some extent, may be a result of the small sample set of 15. Of the four test cases that used one index station, the most accurate and least variable results were produced in the test case where the index station and partial-record station were on the same stream or on streams tributary to each other and where the index station had a larger drainage area than the partial-record station. In

  6. Flow in geothermal wells. Part I. Fundamentals of two-phase flow by the method of irreversible thermodynamics

    SciTech Connect

    Bilicki, Z.; Kestin, J.

    1980-12-01

    A derivation of the field equations for two-phase flow is presented, based on the classical methods of thermodynamics of irreversible processes and resulting in a homogeneous diffusion model. The equations are local and instantaneous and ignore turbulent fluctuations as well as the observable fluctuations of the phase boundaries. (MHR)

  7. A set of parallel, implicit methods for a reconstructed discontinuous Galerkin method for compressible flows on 3D hybrid grids

    SciTech Connect

    Xia, Yidong; Luo, Hong; Frisbey, Megan; Nourgaliev, Robert

    2014-07-01

    A set of implicit methods are proposed for a third-order hierarchical WENO reconstructed discontinuous Galerkin method for compressible flows on 3D hybrid grids. An attractive feature in these methods are the application of the Jacobian matrix based on the P1 element approximation, resulting in a huge reduction of memory requirement compared with DG (P2). Also, three approaches -- analytical derivation, divided differencing, and automatic differentiation (AD) are presented to construct the Jacobian matrix respectively, where the AD approach shows the best robustness. A variety of compressible flow problems are computed to demonstrate the fast convergence property of the implemented flow solver. Furthermore, an SPMD (single program, multiple data) programming paradigm based on MPI is proposed to achieve parallelism. The numerical results on complex geometries indicate that this low-storage implicit method can provide a viable and attractive DG solution for complicated flows of practical importance.

  8. [Application of three heat pulse technique-based methods to determine the stem sap flow].

    PubMed

    Wang, Sheng; Fan, Jun

    2015-08-01

    It is of critical importance to acquire tree transpiration characters through sap flow methodology to understand tree water physiology, forest ecology and ecosystem water exchange. Tri-probe heat pulse sensors, which are widely utilized in soil thermal parameters and soil evaporation measurement, were applied to implement Salix matsudana sap flow density (Vs) measurements via heat-ratio method (HRM), T-Max method (T-Max) and single-probe heat pulse probe (SHPP) method, and comparative analysis was conducted with additional Grainer's thermal diffusion probes (TDP) measured results. The results showed that, it took about five weeks to reach a stable measurement stage after TPHP installation, Vs measured with three methods in the early stage after installation was 135%-220% higher than Vs in the stable measurement stage, and Vs estimated via HRM, T-Max and SHPP methods were significantly linearly correlated with Vs estimated via TDP method, with R2 of 0.93, 0.73 and 0.91, respectively, and R2 for Vs measured by SHPP and HRM reached 0.94. HRM had relatively higher precision in measuring low rates and reverse sap flow. SHPP method seemed to be very promising to measure sap flow for configuration simplicity and high measuring accuracy, whereas it couldn' t distinguish directions of flow. T-Max method had relatively higher error in sap flow measurement, and it couldn' t measure sap flow below 5 cm3 · cm(-2) · h(-1), thus this method could not be used alone, however it could measure thermal diffusivity for calculating sap flow when other methods were imposed. It was recommended to choose a proper method or a combination of several methods to measure stem sap flow, based on specific research purpose.

  9. High speed flow cytometer droplet formation system and method

    DOEpatents

    Van den Engh, Ger

    2000-01-01

    A droplet forming flow cytometer system allows high speed processing without the need for high oscillator drive powers through the inclusion of an oscillator or piezoelectric crystal such as within the nozzle volume or otherwise unidirectionally coupled to the sheath fluid. The nozzle container continuously converges so as to amplify unidirectional oscillations which are transmitted as pressure waves through the nozzle volume to the nozzle exit so as to form droplets from the fluid jet. The oscillator is directionally isolated so as to avoid moving the entire nozzle container so as to create only pressure waves within the sheath fluid. A variation in substance concentration is achieved through a movable substance introduction port which is positioned within a convergence zone to vary the relative concentration of substance to sheath fluid while still maintaining optimal laminar flow conditions. This variation may be automatically controlled through a sensor and controller configuration. A replaceable tip design is also provided whereby the ceramic nozzle tip is positioned within an edge insert in the nozzle body so as to smoothly transition from nozzle body to nozzle tip. The nozzle tip is sealed against its outer surface to the nozzle body so it may be removable for cleaning or replacement.

  10. Numerical simulation methods of incompressible flows and an application to the Space Shuttle main engine

    NASA Technical Reports Server (NTRS)

    Chang, J. L. C.; Kwak, D.; Rogers, S. E.; Yang, R.-J.

    1988-01-01

    This paper discusses incompressible Navier-Stokes solution methods with an emphasis on the pseudocompressibility method. A steady-state flow solver based on the pseudocompressibility approach is then described. This flow solver code has been used to analyze the internal flow in the Space Shuttle main engine hot-gas manifold. Salient features associated with this three-dimensional realistic flow simulation are discussed. Numerical solutions relevant to the current engine analysis and the redesign effort are discussed along with experimental results. This example demonstrates the potential of computational fluid dynamics as a design tool for aerospace applications.

  11. Numerical simulation methods of incompressible flows and an application to the space shuttle main engine

    NASA Technical Reports Server (NTRS)

    Chang, J. L. C.; Kwak, D.; Rogers, S. E.; Yang, R.-J.

    1988-01-01

    Incompressible Navier-Stokes solution methods are discussed with an emphasis on the pseudocompressibility method. A steady-state flow solver based on the pseudocompressibility approach is then described. This flow-solver code was used to analyze the internal flow in the Space Shuttle main engine hot-gas manifold. Salient features associated with this three-dimensional realistic flow simulation are discussed. Numerical solutions relevant to the current engine analysis and the redesign effort are discussed along with experimental results. This example demonstrates the potential of computational fluid dynamics as a design tool for aerospace applications.

  12. Methods for Computationally Efficient Structured CFD Simulations of Complex Turbomachinery Flows

    NASA Technical Reports Server (NTRS)

    Herrick, Gregory P.; Chen, Jen-Ping

    2012-01-01

    This research presents more efficient computational methods by which to perform multi-block structured Computational Fluid Dynamics (CFD) simulations of turbomachinery, thus facilitating higher-fidelity solutions of complicated geometries and their associated flows. This computational framework offers flexibility in allocating resources to balance process count and wall-clock computation time, while facilitating research interests of simulating axial compressor stall inception with more complete gridding of the flow passages and rotor tip clearance regions than is typically practiced with structured codes. The paradigm presented herein facilitates CFD simulation of previously impractical geometries and flows. These methods are validated and demonstrate improved computational efficiency when applied to complicated geometries and flows.

  13. A direct-inverse method for transonic and separated flows about airfoils

    NASA Technical Reports Server (NTRS)

    Carlson, K. D.

    1985-01-01

    A direct-inverse technique and computer program called TAMSEP that can be sued for the analysis of the flow about airfoils at subsonic and low transonic freestream velocities is presented. The method is based upon a direct-inverse nonconservative full potential inviscid method, a Thwaites laminar boundary layer technique, and the Barnwell turbulent momentum integral scheme; and it is formulated using Cartesian coordinates. Since the method utilizes inverse boundary conditions in regions of separated flow, it is suitable for predicing the flowfield about airfoils having trailing edge separated flow under high lift conditions. Comparisons with experimental data indicate that the method should be a useful tool for applied aerodynamic analyses.

  14. On the peculiarities of LDA method in two-phase flows with high concentrations of particles

    NASA Astrophysics Data System (ADS)

    Poplavski, S. V.; Boiko, V. M.; Nesterov, A. U.

    2016-10-01

    Popular applications of laser Doppler anemometry (LDA) in gas dynamics are reviewed. It is shown that the most popular method cannot be used in supersonic flows and two-phase flows with high concentrations of particles. A new approach to implementation of the known LDA method based on direct spectral analysis, which offers better prospects for such problems, is presented. It is demonstrated that the method is suitable for gas-liquid jets. Owing to the progress in laser engineering, digital recording of spectra, and computer processing of data, the method is implemented at a higher technical level and provides new prospects of diagnostics of high-velocity dense two-phase flows.

  15. Potential flow around two-dimensional airfoils using a singular integral method

    NASA Technical Reports Server (NTRS)

    Nguyen, Yves; Wilson, Dennis

    1987-01-01

    The problem of potential flow around two-dimensional airfoils is solved by using a new singular integral method. The potential flow equations for incompressible potential flow are written in a singular integral equation. The equation is solved at N collocation points on the airfoil surface. A unique feature of this method is that the airfoil geometry is specified as an independent variable in the exact integral equation. Compared to other numerical methods, the present calculation procedure is much simpler and gives remarkable accuracy for many body shapes. An advantage of the present method is that it allows the inverse design calculation and the results are extremely accurate.

  16. Newton-Krylov-Schwarz methods in unstructured grid Euler flow

    SciTech Connect

    Keyes, D.E.

    1996-12-31

    Newton-Krylov methods and Krylov-Schwarz (domain decomposition) methods have begun to become established in computational fluid dynamics (CFD) over the past decade. The former employ a Krylov method inside of Newton`s method in a Jacobian-free manner, through directional differencing. The latter employ an overlapping Schwarz domain decomposition to derive a preconditioner for the Krylov accelerator that relies primarily on local information, for data-parallel concurrency. They may be composed as Newton-Krylov-Schwarz (NKS) methods, which seem particularly well suited for solving nonlinear elliptic systems in high-latency, distributed-memory environments. We give a brief description of this family of algorithms, with an emphasis on domain decomposition iterative aspects. We then describe numerical simulations with Newton-Krylov-Schwarz methods on an aerodynamic application emphasizing comparisons with a standard defect-correction approach and subdomain preconditioner consistency.

  17. Non-contrast-enhanced MR hepatic arteriography with time-spatial labeling inversion pulses: comparison of imaging with flow-in and modified flow-out methods.

    PubMed

    Isoda, Hiroyoshi; Furuta, Akihiro; Ohno, Tsuyoshi; Togashi, Kaori

    2017-07-01

    Background In non-contrast-enhanced magnetic resonance (MR) arteriography, the flow-out method using a selective tagging pulse with a long inversion time (TI) and a non-selective inversion recovery pulse with a short TI (the modified flow-out method) might further contribute to the suppression of background signal and facilitate improved visualization of the hepatic artery. However, no report has focused on the image quality of non-contrast-enhanced MR arteriography using the modified flow-out method. Purpose To compare the flow-in method with the modified flow-out method and to determine which method better visualizes the hepatic artery. Material and Methods Twenty-seven healthy volunteers were examined using respiratory-triggered three-dimensional true steady-state free-precession MR with two selective inversion recovery pulses (the flow-in method) or with one tagging pulse and one non-selective inversion recovery pulse (the modified flow-out method). Results Artery-to-liver contrast was significantly increased in the modified flow-out method relative to the flow-in method ( P < 0.05). In all analyses of vessel visibility, non-contrast-enhanced MR arteriography using the modified flow-out method exhibited higher scores than with the flow-in method. With respect to overall image quality, the modified flow-out method was significantly better than the flow-in method ( P < 0.01). Conclusion The modified flow-out method for non-contrast-enhanced MR angiography is a promising technique for visualizing the hepatic artery.

  18. Detection of circulating immune complexes by Raji cell assay: comparison of flow cytometric and radiometric methods

    SciTech Connect

    Kingsmore, S.F.; Crockard, A.D.; Fay, A.C.; McNeill, T.A.; Roberts, S.D.; Thompson, J.M.

    1988-01-01

    Several flow cytometric methods for the measurement of circulating immune complexes (CIC) have recently become available. We report a Raji cell flow cytometric assay (FCMA) that uses aggregated human globulin (AHG) as primary calibrator. Technical advantages of the Raji cell flow cytometric assay are discussed, and its clinical usefulness is evaluated in a method comparison study with the widely used Raji cell immunoradiometric assay. FCMA is more precise and has greater analytic sensitivity for AHG. Diagnostic sensitivity by the flow cytometric method is superior in systemic lupus erythematosus (SLE), rheumatoid arthritis, and vasculitis patients: however, diagnostic specificity is similar for both assays, but the reference interval of FCMA is narrower. Significant correlations were found between CIC levels obtained with both methods in SLE, rheumatoid arthritis, and vasculitis patients and in longitudinal studies of two patients with cerebral SLE. The Raji cell FCMA is recommended for measurement of CIC levels to clinical laboratories with access to a flow cytometer.

  19. Flow Simulation Around Cambered Airfoil by Using Conformal Mapping and Intermediate Domain in Lattice Boltzmann Method

    NASA Astrophysics Data System (ADS)

    Poozesh, Amin; Mirzaei, Masoud

    2017-01-01

    In this paper the developed interpolation lattice Boltzmann method is used for simulation of unsteady fluid flow. It combines the desirable features of the lattice Boltzmann and the Joukowski transformation methods. This approach has capability to simulate flow around curved boundary geometries such as airfoils in a body fitted grid system. Simulation of unsteady flow around a cambered airfoil in a non-uniform grid for the first time is considered to show the capability of this method for modeling of fluid flow around complex geometries and complicated long-term periodic flow phenomena. The developed solver is also coupled with a fast adaptive grid generator. In addition, the new approach retains all the advantages of the standard lattice Boltzmann method. The Strouhal number, the pressure, the drag and the lift coefficients obtained from the simulations agree well with classical computational fluid dynamics simulations. Numerical studies for various test cases illustrate the strength of this new approach.

  20. Assessment of three numerical methods for the computation of a low-density plume flow

    NASA Technical Reports Server (NTRS)

    Penko, Paul F.; Riley, Ben R.; Boyd, Iain D.

    1993-01-01

    Results from three numerical methods including one based on the Navier-Stokes equations, one based on kinetic theory using the DSMC method, and one based on the Boltzmann equation with a Krook-type collision term are compared to each other and to experimental data for a model problem of heated nitrogen flow in a conical nozzle expanding into a vacuum. The problem simulates flow in a resistojet, a low-thrust, electrothermal rocket. The continuum method is applied to both the internal flow and near-field plume. The DSMC and Boltzmann methods are applied primarily to the plume. Experimental measurements of Pitot pressure and flow angle, taken with an apparatus that duplicates the model nozzle flow, are used in the comparisons.

  1. Evaluation of environmental flow requirements using eco-hydrologic-hydraulic methods in perennial rivers.

    PubMed

    Abdi, Reza; Yasi, Mehdi

    2015-01-01

    The assessment of environmental flows in rivers is of vital importance for preserving riverine ecosystem processes. This paper addresses the evaluation of environmental flow requirements in three reaches along a typical perennial river (the Zab transboundary river, in north-west Iran), using different hydraulic, hydrological and ecological methods. The main objective of this study came from the construction of three dams and inter-basin transfer of water from the Zab River to the Urmia Lake. Eight hydrological methods (i.e. Tennant, Tessman, flow duration curve analysis, range of variability approach, Smakhtin, flow duration curve shifting, desktop reserve and 7Q2&10 (7-day low flow with a 2- and 10-year return period)); two hydraulic methods (slope value and maximum curvature); and two habitat simulation methods (hydraulic-ecologic, and Q Equation based on water quality indices) were used. Ecological needs of the riverine key species (mainly Barbus capito fish), river geometries, natural flow regime and the environmental status of river management were the main indices for determining the minimum flow requirements. The results indicate that the order of 35%, 17% and 18% of the mean annual flow are to be maintained for the upper, middle and downstream river reaches, respectively. The allocated monthly flow rates in the three Dams steering program are not sufficient to preserve the Zab River life.

  2. Method and apparatus for acoustically monitoring the flow of suspended solid particulate matter

    DOEpatents

    Roach, Paul D.; Raptis, Apostolos C.

    1982-01-01

    A method and apparatus for monitoring char flow in a coal gasifier system cludes flow monitor circuits which measure acoustic attenuation caused by the presence of char in a char line and provide a char flow/no flow indication and an indication of relative char density. The flow monitor circuits compute the ratio of signals in two frequency bands, a first frequency band representative of background noise, and a second higher frequency band in which background noise is attenuated by the presence of char. Since the second frequency band contains higher frequencies, the ratio can be used to provide a flow/no flow indication. The second band can also be selected so that attenuation is monotonically related to particle concentration, providing a quantitative measure of char concentration.

  3. Laboratory Evaluation of Air Flow Measurement Methods for Residential HVAC Returns

    SciTech Connect

    Walker, Iain; Stratton, Chris

    2015-07-01

    This project improved the accuracy of air flow measurements used in commissioning California heating and air conditioning systems in Title 24 (Building and Appliance Efficiency Standards), thereby improving system performance and efficiency of California residences. The research team at Lawrence Berkeley National Laboratory addressed the issue that typical tools used by contractors in the field to test air flows may not be accurate enough to measure return flows used in Title 24 applications. The team developed guidance on performance of current diagnostics as well as a draft test method for use in future evaluations. The series of tests performed measured air flow using a range of techniques and devices. The measured air flows were compared to reference air flow measurements using inline air flow meters built into the test apparatus. The experimental results showed that some devices had reasonable results (typical errors of 5 percent or less) but others had much bigger errors (up to 25 percent).

  4. A novel flow cytometric method to assess inflammasome formation.

    PubMed

    Sester, David P; Thygesen, Sara J; Sagulenko, Vitaliya; Vajjhala, Parimala R; Cridland, Jasmyn A; Vitak, Nazarii; Chen, Kaiwen W; Osborne, Geoffrey W; Schroder, Kate; Stacey, Katryn J

    2015-01-01

    Inflammasomes are large protein complexes induced by a wide range of microbial, stress, and environmental stimuli that function to induce cell death and inflammatory cytokine processing. Formation of an inflammasome involves dramatic relocalization of the inflammasome adapter protein apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) into a single speck. We have developed a flow cytometric assay for inflammasome formation, time of flight inflammasome evaluation, which detects the change in ASC distribution within the cell. The transit of ASC into the speck is detected by a decreased width or increased height of the pulse of emitted fluorescence. This assay can be used to quantify native inflammasome formation in subsets of mixed cell populations ex vivo. It can also provide a rapid and sensitive technique for investigating molecular interactions in inflammasome formation, by comparison of wild-type and mutant proteins in inflammasome reconstitution experiments.

  5. Computation of steady axisymmetric flow using a one-dimensional time-dependent method

    NASA Technical Reports Server (NTRS)

    Walitt, L.

    1974-01-01

    An iterative numerical method for computing steady, three dimensional, viscous, compressible flow fields, about aerodynamic bodies was studied. In order to develop the iterative method economically, the primary emphasis was directed towards supersonic, axisymmetric flow. However, the technique readily extends to three spatial dimensions. The viscous flow field about a cone-cylinder-flare body was calculated and compared to existing experimental data. Numerical predictions of the cone boundary layer and the flow field shock wave structure agreed with corresponding measurements. A separation was calculated at the cylinder-flare junction in six iterations; however, the size of the vortex did not correspond to the measured size. It was estimated that fifty iterations would be required to properly define the vortex. It was concluded that the iteration technique is of limited value for plane two dimensional and axisymmetrix flows, but of great value for three-dimensional flows.

  6. Adaptive Discontinuous Evolution Galerkin Method for Dry Atmospheric Flow

    DTIC Science & Technology

    2013-04-02

    standard one-dimensional approximate Riemann solver used for the flux integration demonstrate better stability, accuracy as well as reliability of the...discontinuous evolution Galerkin method for dry atmospheric convection. Comparisons with the standard one-dimensional approximate Riemann solver used...instead of a standard one- dimensional approximate Riemann solver, the flux integration within the discontinuous Galerkin method is now realized by

  7. Apparatus and method for using radar to evaluate wind flow fields for wind energy applications

    DOEpatents

    Schroeder, John; Hirth, Brian; Guynes, Jerry

    2017-02-21

    The present invention provides an apparatus and method for obtaining data to determine one or more characteristics of a wind flow field using one or more radars. Data is collected from the one or more radars, and analyzed to determine the one or more characteristics of the wind flow field. The one or more radars are positioned to have a portion of the wind flow field within a scanning sector of the one or more radars.

  8. Three dimensional hypersonic flow simulations with the CSCM implicit upwind Navier-Stokes method. [Conservative Supra-Characteristic Method

    NASA Technical Reports Server (NTRS)

    Bardina, Jorge; Lombard, C. K.

    1987-01-01

    The Bardina and Lombard (1985) three-dimensional CSCM Navier-Stokes method is presently extended to the simulation of complex hypersonic reentry vehicle external flows at angle of attack. The robust stability of the method derives from the combination of conservative implicit upwind flux difference splitting with a three-dimensional diagonally-dominant approximate factorization and relaxation scheme and characteristic-based implicit boundary approximations. The method's efficiency derives from an implicit symmetric Gauss-Seidel 'method of planes' relaxation scheme with alternating directional space marching sweeps along the flow coordinate direction.

  9. Method and apparatus for cold gas reinjection in through-flow and reverse-flow wave rotors

    NASA Technical Reports Server (NTRS)

    Nalim, M. Razi (Inventor); Paxson, Daniel E. (Inventor)

    1999-01-01

    A method and apparatus for cold gas reinjection in through-flow and reverse-flow wave rotors having a plurality of channels formed around a periphery thereof. A first port injects a supply of cool air into the channels. A second port allows the supply of cool air to exit the channels and flow to a combustor. A third port injects a supply of hot gas from the combustor into the channels. A fourth port allows the supply of hot gas to exit the channels and flow to a turbine. A diverting port and a reinjection port are connected to the second and third ports, respectively. The diverting port diverts a portion of the cool air exiting through the second port as reinjection air. The diverting port is fluidly connected to the reinjection port which reinjects the reinjection air back into the channels. The reinjection air evacuates the channels of the hot gas resident therein and cools the channel walls, a pair of end walls of the rotor, ducts communicating with the rotor and subsequent downstream components. In a second embodiment, the second port receives all of the cool air exiting the channels and the diverting port diverts a portion of the cool air just prior to the cool air flowing to the combustor.

  10. Field method for separating the contribution of surface-connected preferential flow pathways from flow through the soil matrix

    NASA Astrophysics Data System (ADS)

    Sanders, Emily C.; Abou Najm, Majdi R.; Mohtar, Rabi H.; Kladivko, Eileen; Schulze, Darrell

    2012-04-01

    Liquid latex was used as a method to seal visible surface-connected preferential flow pathways (PFPs) in the field in an effort to block large surface-connected preferential flow and force water to move through the soil matrix. The proposed approach allows for the quantification of the contribution of large surface-connected cracks and biological pores to infiltration at various soil moisture states. Experiments were conducted in a silty clay loam soil in a field under a no-till corn-soybean rotation planted to corn. Surface intake rates under ponding were measured using a simplified falling head technique under two scenarios: (1) natural soil conditions with unaltered PFPs and (2) similar soil conditions with latex-sealed large macropores at the surface. Results indicated that the contribution of flow from large surface-connected macropores to overall surface intake rates varied from approximately 34% to 99% depending on the initial moisture content and macroporosity present. However, evidence of preferential flow continued to appear in latex-sealed plots, suggesting significant contributions to preferential flow from smaller structural macropores, particularly in two out of four tests where no significant differences were observed between control and latex-sealed plots.

  11. Apparatus and method for constant flow oxidizing of organic materials

    DOEpatents

    Surma, Jeffrey E.; Nelson, Norvell; Steward, G. Anthony; Bryan, Garry H.

    1999-01-01

    The invention is a method and apparatus using high cerium concentration in the anolyte of an electrochemical cell to oxidize organic materials. The method and apparatus further use an ultrasonic mixer to enhance the oxidation rate of the organic material in the electrochemical cell. A reaction vessel provides an advantage of independent reaction temperature control and electrochemical cell temperature control. A separate or independent reaction vessel may be used without an ultrasonic mixer to oxidize gaseous phase organic materials.

  12. Improved Soft Abrasive Flow Finishing Method Based on Turbulent Kinetic Energy Enhancing

    NASA Astrophysics Data System (ADS)

    LI, Jun; JI, Shiming; TAN, Dapeng

    2017-03-01

    Soft abrasive flow(SAF) finishing can process the irregular geometric surfaces, but with the matter of low processing efficiency. To address the issue, an improved SAF finishing method based on turbulent kinetic energy enhancing is proposed. A constrained flow passage with serration cross-section is constructed to increase the turbulence intensity. Taking the constrained flow passage as the objective, a two-phase fluid dynamic model is set up by using particle trajectory model and standard k-ɛ turbulence model, and the flow field characteristics of the flow passage are acquired. The numerical results show that the serration flow passage can enhance the turbulence intensity, uniform the particles distribution, and increase the particle concentration near the bottom wall. The observation results by particle image velocimetry(PIV) show that the internal vortex structures are formed in flow passage, and the abrasive flow takes on turbulence concentrating phenomenon in near-wall region. The finishing experiments prove that the proposed method can obtain better surface uniformity, and the processing efficiency can be improved more 35%. This research provides an abrasive flow modeling method to reveal the particle motion regulars, and can offer references to the technical optimization of fluid-based precision processing.

  13. Methods for estimating selected low-flow frequency statistics and harmonic mean flows for streams in Iowa

    USGS Publications Warehouse

    Eash, David A.; Barnes, Kimberlee K.

    2012-01-01

    -least-squares equations developed for estimating the harmonic-mean-flow statistic for each of the three regions range from 66.4 to 80.4 percent. The regression equations are applicable only to stream sites in Iowa with low flows not significantly affected by regulation, diversion, or urbanization and with basin characteristics within the range of those used to develop the equations. If the equations are used at ungaged sites on regulated streams, or on streams affected by water-supply and agricultural withdrawals, then the estimates will need to be adjusted by the amount of regulation or withdrawal to estimate the actual flow conditions if that is of interest. Caution is advised when applying the equations for basins with characteristics near the applicable limits of the equations and for basins located in karst topography. A test of two drainage-area ratio methods using 31 pairs of streamgages, for the annual 7-day mean low-flow statistic for a recurrence interval of 10 years, indicates a weighted drainage-area ratio method provides better estimates than regional regression equations for an ungaged site on a gaged stream in Iowa when the drainage-area ratio is between 0.5 and 1.4. These regression equations will be implemented within the U.S. Geological Survey StreamStats web-based geographic-information-system tool. StreamStats allows users to click on any ungaged site on a river and compute estimates of the seven selected statistics; in addition, 90-percent prediction intervals and the measured basin characteristics for the ungaged sites also are provided. StreamStats also allows users to click on any streamgage in Iowa and estimates computed for these seven selected statistics are provided for the streamgage.

  14. Three-dimensional flow analysis inside turbomachinery stages with steady and unsteady Navier-Stokes method

    NASA Technical Reports Server (NTRS)

    Copenhaver, W. W.; Puterbauch, S. L.; Hah, C.

    1993-01-01

    This study presents a numerical method for solving the three-dimensional, Navier-Stokes equations for unsteady, viscous flow through multiple turbomachinery blade rows. The method solves the fully three-dimensional Navier-Stokes equations with an implicit scheme which is based on a control volume approach. A two-equation turbulence model with a low Reynolds number modification is employed in the present study. A third-order accurate upwinding scheme is used to approximate convection terms while a second order accurate central difference scheme is used for the discretization of viscous terms. A second-order accurate scheme is employed for the temporal discretization. The numerical method is applied to study the unsteady flow field of a subsonic turbine stage and the unsteady flow field inside a transonic, high-through-flow, axial compressor stage. The stage calculation is performed by coupling the stator and the rotor flow fields at each time step through an over-laid grid.

  15. The Ghost Cell Method for Inviscid Compressible Flow on Adaptive Tree Cartesian Grids

    NASA Astrophysics Data System (ADS)

    Liu, Jianming; Zhao, Ning; Hu, Ou

    2010-05-01

    In this paper, a new immersed boundary algorithm is developed by combining the ghost cell method with adaptive tree Cartesian grid method. Furthermore, the presented method has been successfully used to evaluate an inviscid compressible flow with immersed boundary. Numerical example shows the present method be effective.

  16. Modeling of near-continuum flows using the direct simulation Monte Carlo method

    NASA Astrophysics Data System (ADS)

    Lohn, P. D.; Haflinger, D. E.; McGregor, R. D.; Behrens, H. W.

    1990-06-01

    The direct simulation Monte Carlo (DSMC) method is used to model the flow of a hypersonic stream about a wedge. The Knudsen number of 0.00075 puts the flow into the continuum category and hence is a challenge for the DSMC method. The modeled flowfield is shown to agree extremely well with the experimental measurements in the wedge wake taken by Batt (1967). This experimental confirmation serves as a rigorous validation of the DSMC method and provides guidelines for computations of near-continuum flows.

  17. The development of three-dimensional adjoint method for flow control with blowing in convergent-divergent nozzle flows

    NASA Astrophysics Data System (ADS)

    Sikarwar, Nidhi

    The noise produced by the low bypass ratio turbofan engines used to power fighter aircraft is a problem for communities near military bases and for personnel working in close proximity to the aircraft. For example, carrier deck personnel are subject to noise exposure that can result in Noise-Induced Hearing Loss which in-turn results in over a billion dollars of disability payments by the Veterans Administration. Several methods have been proposed to reduce the jet noise at the source. These methods include microjet injection of air or water downstream of the jet exit, chevrons, and corrugated nozzle inserts. The last method involves the insertion of corrugated seals into the diverging section of a military-style convergent-divergent jet nozzle (to replace the existing seals). This has been shown to reduce both the broadband shock-associated noise as well as the mixing noise in the peak noise radiation direction. However, the original inserts were designed to be effective for a take-off condition where the jet is over-expanded. The nozzle performance would be expected to degrade at other conditions, such as in cruise at altitude. A new method has been proposed to achieve the same effects as corrugated seals, but using fluidic inserts. This involves injection of air, at relatively low pressures and total mass flow rates, into the diverging section of the nozzle. These fluidic inserts" deflect the flow in the same way as the mechanical inserts. The fluidic inserts represent an active control method, since the injectors can be modified or turned off depending on the jet operating conditions. Noise reductions in the peak noise direction of 5 to 6 dB have been achieved and broadband shock-associated noise is effectively suppressed. There are multiple parameters to be considered in the design of the fluidic inserts. This includes the number and location of the injectors and the pressures and mass flow rates to be used. These could be optimized on an ad hoc basis with

  18. A direct and inverse boundary layer method for subsonic flow over delta wings

    NASA Technical Reports Server (NTRS)

    Woodson, S. H.; Dejarnette, F. R.

    1986-01-01

    A new inverse boundary layer method is developed and applied to incompressible flows with laminar separation and reattachment. Test cases for two dimensional flows are computed and the results are compared with those of other inverse methods. One advantage of the present method is that the calculation of the inviscid velocities may be determined at each marching step without having to iterate. The inverse method was incorporated with the direct method to calculate the incompressible, conical flow over a slender delta wing at incidence. The location of the secondary separation line on the leeward surface of the wing is determined and compared with experiment for a unit aspect ratio wing at 20.5 deg incidence. The viscous flow in the separated region was calculated using prescribed skin friction coefficients.

  19. A pressure correction method for the calculation of compressible chemical reacting flows

    NASA Technical Reports Server (NTRS)

    Chen, Z. J.; Chen, C. P.; Chen, Y. S.

    1992-01-01

    A recently developed noniterative method for the solution of the transient fluid flow equations at all speed is extended to handle chemical reacting flows. The species conservation equations are loosely coupled into the predictor/multicorrector sequence of the solution procedure. A split-operator method separates the chemical kinetics terms from the fluid-dynamical terms, as well as an implicit differencing method enhance the numerical stability. The method was applied for turbulent diffusion flame calculations and for the analyses of high pressure, axisymmetric turbulent hypersonic nozzle flows. The diffusion flame results were compared with a similar pressure method for fast chemistry integration scheme without operator-splitting. Simulations of the nozzle flow indicated that the nonideal intermolecular effects must be included in the analysis and design of high pressure hypersonic nozzle.

  20. Direct Numerical Simulation of Incompressible Pipe Flow Using a B-Spline Spectral Method

    NASA Technical Reports Server (NTRS)

    Loulou, Patrick; Moser, Robert D.; Mansour, Nagi N.; Cantwell, Brian J.

    1997-01-01

    A numerical method based on b-spline polynomials was developed to study incompressible flows in cylindrical geometries. A b-spline method has the advantages of possessing spectral accuracy and the flexibility of standard finite element methods. Using this method it was possible to ensure regularity of the solution near the origin, i.e. smoothness and boundedness. Because b-splines have compact support, it is also possible to remove b-splines near the center to alleviate the constraint placed on the time step by an overly fine grid. Using the natural periodicity in the azimuthal direction and approximating the streamwise direction as periodic, so-called time evolving flow, greatly reduced the cost and complexity of the computations. A direct numerical simulation of pipe flow was carried out using the method described above at a Reynolds number of 5600 based on diameter and bulk velocity. General knowledge of pipe flow and the availability of experimental measurements make pipe flow the ideal test case with which to validate the numerical method. Results indicated that high flatness levels of the radial component of velocity in the near wall region are physical; regions of high radial velocity were detected and appear to be related to high speed streaks in the boundary layer. Budgets of Reynolds stress transport equations showed close similarity with those of channel flow. However contrary to channel flow, the log layer of pipe flow is not homogeneous for the present Reynolds number. A topological method based on a classification of the invariants of the velocity gradient tensor was used. Plotting iso-surfaces of the discriminant of the invariants proved to be a good method for identifying vortical eddies in the flow field.