The Use of Flow-Injection Analysis with Chemiluminescence Detection of Aqueous Ferrous Iron in Waters Containing High Concentrations of Organic Compounds

PubMed Central

Borman, Christopher J.; Sullivan, B. Patrick; Eggleston, Carrick M.; Colberg, Patricia J. S.

2009-01-01

An evaluation of flow-injection analysis with chemiluminescence detection (FIA-CL) to quantify Fe2+(aq) in freshwaters was performed. Iron-coordinating and/or iron-reducing compounds, dissolved organic matter (DOM), and samples from two natural water systems were used to amend standard solutions of Fe2+(aq). Slopes of the response curves from ferrous iron standards (1 – 100 nM) were compared to the response curves of iron standards containing the amendments. Results suggest that FIA-CL is not suitable for systems containing ascorbate, hydroxylamine, cysteine or DOM. Little or no change in sensitivity occurred in solutions of oxalate and glycine or in natural waters with little organic matter. PMID:22408532

Biosensing of glucose in flow injection analysis system based on glucose oxidase-quantum dot modified pencil graphite electrode.

PubMed

Sağlam, Özlem; Kızılkaya, Bayram; Uysal, Hüseyin; Dilgin, Yusuf

2016-01-15

A novel amperometric glucose biosensor was proposed in flow injection analysis (FIA) system using glucose oxidase (GOD) and Quantum dot (ZnS-CdS) modified Pencil Graphite Electrode (PGE). After ZnS-CdS film was electrochemically deposited onto PGE surface, GOD was immobilized on the surface of ZnS-CdS/PGE through crosslinking with chitosan (CT). A pair of well-defined reversible redox peak of GOD was observed at GOD/CT/ZnS-CdS/PGE based on enzyme electrode by direct electron transfer between the protein and electrode. Further, obtained GOD/CT/ZnS-CdS/PGE offers a disposable, low cost, selective and sensitive electrochemical biosensing of glucose in FIA system based on the decrease of the electrocatalytic response of the reduced form of GOD to dissolved oxygen. Under optimum conditions (flow rate, 1.3mL min(-1); transmission tubing length, 10cm; injection volume, 100μL; and constant applied potential, -500mV vs. Ag/AgCl), the proposed method displayed a linear response to glucose in the range of 0.01-1.0mM with detection limit of 3.0µM. The results obtained from this study would provide the basis for further development of the biosensing using PGE based FIA systems. Copyright © 2015 Elsevier B.V. All rights reserved.

Flow injection analysis of organic peroxide explosives using acid degradation and chemiluminescent detection of released hydrogen peroxide.

PubMed

Mahbub, Parvez; Zakaria, Philip; Guijt, Rosanne; Macka, Mirek; Dicinoski, Greg; Breadmore, Michael; Nesterenko, Pavel N

2015-10-01

The applicability of acid degradation of organic peroxides into hydrogen peroxide in a pneumatically driven flow injection system with chemiluminescence reaction with luminol and Cu(2+) as a catalyst (FIA-CL) was investigated for the fast and sensitive detection of organic peroxide explosives (OPEs). The target OPEs included hexamethylene triperoxide diamine (HMTD), triacetone triperoxide (TATP) and methylethyl ketone peroxide (MEKP). Under optimised conditions maximum degradations of 70% and 54% for TATP and HMTD, respectively were achieved at 162 µL min(-1), and 9% degradation for MEKP at 180 µL min(-1). Flow rates were precisely controlled in this single source pneumatic pressure driven multi-channel FIA system by model experiments on mixing of easily detectable component solutions. The linear range for detection of TATP, HMTD and H2O2 was 1-200 µM (r(2)=0.98-0.99) at both flow rates, while that for MEKP was 20-200 µM (r(2)=0.97) at 180 µL min(-1). The detection limits (LODs) obtained were 0.5 µM for TATP, HMTD and H2O2 and 10 µM for MEKP. The detection times varied from 1.5 to 3 min in this FIA-CL system. Whilst the LOD for H2O2 was comparable with those reported by other investigators, the LODs and analysis times for TATP and HMTD were superior, and significantly, this is the first time the detection of MEKP has been reported by FIA-CL. Copyright © 2015 Elsevier B.V. All rights reserved.

Evaluation of a direct injection nebulizer interface for flow injection analysis and high performance liquid chromatography with inductively coupled plasma-atomic emission spectroscopic detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

LaFreniere, K.E.

A direct injection nebulizer (DIN) was designed, developed, and evaluated to determine its potential utilization as an effective interface for flow injection analysis (FIA) and high performance liquid chromatography (HPLC) coupled with inductively coupled plasma-atomic emission spectroscopic detection. The analytical figures of merit for the DIN when used as an interface for FIA-ICP-AES were found to be comparable to or better than those obtained with conventional pneumatic nebulization in terms of limits of detection (LODs), reproducibility, linearity, and interelement effects. Stable plasma operation was maintained for the DIN sample introduction of a variety of pure organic solvents, including acetonitrile, methanol,more » methylisobutylketone, and pyridine. The HPLC-DIN-ICP-AES facility was specifically applied for the speciation of inorganic and organometallic species contained in synthetic mixtures, vanilla extracts, and a variety of energy-related materials, such as shale oil process water, coal extracts, shale oil, crude oil, and an SRC II. Suggestions for future research are also considered. 227 refs., 44 figs., 15 tabs.« less

Determination of Total Selenium in Infant Formulas: Comparison of the Performance of FIA and MCFA Flow Systems

PubMed Central

Pistón, Mariela; Knochen, Moisés

2012-01-01

Two flow methods, based, respectively, on flow-injection analysis (FIA) and on multicommutated flow analysis (MCFA), were compared with regard to their use for the determination of total selenium in infant formulas by hydride-generation atomic absorption spectrometry. The method based on multicommutation provided lower detection and quantification limits (0.08 and 0.27 μg L−1 compared to 0.59 and 1.95 μ L−1, resp.), higher sampling frequency (160 versus. 70 samples per hour), and reduced reagent consumption. Linearity, precision, and accuracy were similar for the two methods compared. It was concluded that, while both methods proved to be appropriate for the purpose, the MCFA-based method exhibited a better performance. PMID:22505923

Speciation of chromium (VI) and total chromium determination in welding dust samples by flow-injection analysis coupled to atomic absorption spectrometry.

PubMed

Girard, L; Hubert, J

1996-11-01

We have studied the speciation of chromium (VI) in stainless-steel welding dusts. The approach used for the analysis of Cr(VI) and total Cr relies on a flow-injection analyzer (FIA) equipped with two different sequential detectors. The system measures Cr(VI). by colorimetry (with 1,5-diphenyl carbohydrazide) and total chromium content by flame atomic absorption spectroscopy (AAS). The extraction of the samples of welding-fume dusts is achieved in a buffer solution (acetic acid and sodium acetate at pH 4). This extraction procedure gives a 96% recovery of chromium (VI). The FIA-AAS system that has been described is also more sensitive, has a lower detection limit (0.005 mug ml(-1)) and gives a better precision (< 1%) than other equivalent systems that have been previously described.

The use of a polymer inclusion membrane in flow injection analysis for the on-line separation and determination of zinc.

PubMed

Zhang, Lujia L; Cattrall, Robert W; Kolev, Spas D

2011-06-15

This paper reports the first use of a polymer inclusion membrane (PIM) for on-line separation in flow injection analysis (FIA) involving simultaneous extraction and back-extraction. The FIA system containing the PIM separation module was used for the determination of Zn(II) in aqueous samples in the presence of Mg(II), Ca(II), Cd(II), Co(II), Ni(II), Cu(II), and Fe(III). The Fe(III) and Cu(II) interferences were eliminated by off-line precipitation with phosphate and on-line complexation with chloride, respectively. The concentration of Zn(II) was determined spectrophotometrically using 4-(2-pyridylazo) resorcinol (PAR). The optimal composition of the PIM consisted of 40% (m/m) di(2-ethlyhexyl) phosphoric acid (D2EHPA) as carrier, 10% (m/m) dioctyl phthalate (DOP) as plasticizer and 50% (m/m) poly(vinyl chloride) (PVC) as the base polymer. The optimized FIA system was characterized by a linear calibration curve in the range from 1.0 to 30.0 mg L(-1) Zn(II), a detection limit of 0.05 mg L(-1) and a relative standard deviation of 3.4% with a sampling rate of 4h(-1). Reproducible results were obtained for 20 replicate injections over a 5h period which demonstrated a good membrane stability. The FIA system was applied to the determination of Zn(II) in pharmaceuticals and samples from the galvanizing industry and very good agreement with atomic absorption spectrometry was obtained. Copyright © 2011 Elsevier B.V. All rights reserved.

Evaluation of a direct injection nebulizer interface for flow injection analysis and high performance liquid chromatography with inductively coupled plasma-atomic emission spectroscopic detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

LaFreniere, K.E.

A direct injection nebulizer (DIN) was designed, developed and evaluated to determine its potential utilization as an effective interface for flow injection analysis (FIA) and high performance liquid chromatography (HPLC) coupled with inductively coupled plasma-atomic emission spectroscopic detection. The analytical figures of merit for the DIN when used as an interface for FIA-ICP-AES were found to be comparable to or better than those obtained with conventional pneumatic nebulization in terms of limits of detection (LODs), reproducibility, linearity, and interelement effects. In the HPLC mode, the LODDs were found to be comparable to those obtained by continuous-flow sample introduction into themore » ICP, or inferior by up to only a factor of four. Stable plasma operation was maintained for the DIN sample introduction of a variety of pure organic solvents, including acetonitrile, methanol, methyl-isobutylketone, and pyridine. The HPLC-DIN-ICP-AES facility was specifically applied for the speciation of inorganic and organo-metallic species contained in synthetic mixtures, vanilla extracts and a variety of energy-related materials, such as shale oil process water, coal extracts, shale oil, crude oil, and an SRC II. Suggestions for future research are also considered.« less

Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.

PubMed

Bell-Vlasov, Andrea K; Zajda, Joanna; Eldourghamy, Ayman; Malinowska, Elzbieta; Meyerhoff, Mark E

2014-04-15

A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined. The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt. The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition. It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h. Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse. The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.

Flow Injection Analysis with Electrochemical Detection for Rapid Identification of Platinum-Based Cytostatics and Platinum Chlorides in Water

PubMed Central

Kominkova, Marketa; Heger, Zbynek; Zitka, Ondrej; Kynicky, Jindrich; Pohanka, Miroslav; Beklova, Miroslava; Adam, Vojtech; Kizek, Rene

2014-01-01

Platinum-based cytostatics, such as cisplatin, carboplatin or oxaliplatin are widely used agents in the treatment of various types of tumors. Large amounts of these drugs are excreted through the urine of patients into wastewaters in unmetabolised forms. This phenomenon leads to increased amounts of platinum ions in the water environment. The impacts of these pollutants on the water ecosystem are not sufficiently investigated as well as their content in water sources. In order to facilitate the detection of various types of platinum, we have developed a new, rapid, screening flow injection analysis method with electrochemical detection (FIA-ED). Our method, based on monitoring of the changes in electrochemical behavior of analytes, maintained by various pH buffers (Britton-Robinson and phosphate buffer) and potential changes (1,000, 1,100 and 1,200 mV) offers rapid and cheap selective determination of platinum-based cytostatics and platinum chlorides, which can also be present as contaminants in water environments. PMID:24499878

Rapid Quantitative Analysis of Multiple Explosive Compound Classes on a Single Instrument via Flow-Injection Analysis Tandem Mass Spectrometry.

PubMed

Ostrinskaya, Alla; Kunz, Roderick R; Clark, Michelle; Kingsborough, Richard P; Ong, Ta-Hsuan; Deneault, Sandra

2018-05-24

A flow-injection analysis tandem mass spectrometry (FIA MSMS) method was developed for rapid quantitative analysis of 10 different inorganic and organic explosives. Performance is optimized by tailoring the ionization method (APCI/ESI), de-clustering potentials, and collision energies for each specific analyte. In doing so, a single instrument can be used to detect urea nitrate, potassium chlorate, 2,4,6-trinitrotoluene, 2,4,6-trinitrophenylmethylnitramine, triacetone triperoxide, hexamethylene triperoxide diamine, pentaerythritol tetranitrate, 1,3,5-trinitroperhydro-1,3,5-triazine, nitroglycerin, and octohy-dro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine with sensitivities all in the picogram per milliliter range. In conclusion, FIA APCI/ESI MSMS is a fast (<1 min/sample), sensitive (~pg/mL LOQ), and precise (intraday RSD < 10%) method for trace explosive detection that can play an important role in criminal and attributional forensics, counterterrorism, and environmental protection areas, and has the potential to augment or replace several of the existing explosive detection methods. © 2018 American Academy of Forensic Sciences.

A New Microfluidic Polymer Chip with an Embedded Cationic Surfactant Ion-selective Optode as a Detector for the Determination of Cationic Surfactants.

PubMed

Ashagre, Mekonnen Abiyot; Masadome, Takashi

2018-01-01

A new microfluidic polymer chip with an embedded cationic surfactant (CS) ion-selective optode (CS-optode) as a detector of flow-injection analysis (FIA) for the determination of CSs was developed. The optode sensing membrane is based on a poly(vinyl chloride) membrane plasticized with 2-nitrophenyl octyl ether containing tetrabromophenolphthalein ethyl ester. Under the optimal flow conditions of the FIA system, the CS-optode showed a good linear relationship between the peak heights in the absorbance, and the concentrations of CS in a concentration range from 50 to 400 μmol dm -3 . The sample throughput of the present system for the determination of a CS ion (300 μmol dm -3 zephiramine) was ca. 11 samples h -1 . The proposed FIA system was applied to determine the level of CS in dental rinses.

Toward a high-throughput method for determining vicine and convicine levels in faba bean seeds using flow injection analysis combined with tandem mass spectrometry.

PubMed

Purves, Randy W; Khazaei, Hamid; Vandenberg, Albert

2018-08-01

Although faba bean provides environmental and health benefits, vicine and convicine (v-c) limit its use as a source of vegetable protein. Crop improvement efforts to minimize v-c concentration require low-cost, rapid screening methods to distinguish between high and low v-c genotypes to accelerate development of new cultivars and to detect out-crossing events. To assist crop breeders, we developed a unique and rapid screening method that uses a 60 s instrumental analysis step to accurately distinguish between high and low v-c genotypes. The method involves flow injection analysis (FIA) coupled with tandem mass spectrometry (i.e., selective reaction monitoring, SRM). Using seeds with known v-c levels as calibrants, measured v-c levels were comparable with liquid chromatography (LC)-SRM results and the method was used to screen 370 faba bean genotypes. Widespread use of FIA-SRM will accelerate breeding of low v-c faba bean, thereby alleviating concerns about anti-nutritional effects of v-c in this crop. Copyright © 2018 Elsevier Ltd. All rights reserved.

Ferrum nano particles and multiwall carbon nano tubes based electrode as FIA detector for determination of amino acids in hypothalamus microdialysis fluids

NASA Astrophysics Data System (ADS)

Sun, L.; Wang, J.; Wang, Y. T.; Yu, L.; Peng, H.; Zhu, J. Z.

2017-01-01

An amperometric electrode based on multiwall carbon nanotubes (MWCNTs) and Fe nanoparticles (NPs) has been successfully fabricated. Combined with Flow Injection Analysis (FIA) and chromatography separation column, the electrode exhibits linear response in the concentration range of 0.1 -12 μM and the sensitivity of 30.0 nA μM-1 for most of amino acids. The determination of 17 amino acids in the hypothalamus microdialysis fluids of guinea pigs, illustrates that the electrode is a powerful tool to investigate physiology and pathology mechanisms

Flow injection analysis simulations and diffusion coefficient determination by stochastic and deterministic optimization methods.

PubMed

Kucza, Witold

2013-07-25

Stochastic and deterministic simulations of dispersion in cylindrical channels on the Poiseuille flow have been presented. The random walk (stochastic) and the uniform dispersion (deterministic) models have been used for computations of flow injection analysis responses. These methods coupled with the genetic algorithm and the Levenberg-Marquardt optimization methods, respectively, have been applied for determination of diffusion coefficients. The diffusion coefficients of fluorescein sodium, potassium hexacyanoferrate and potassium dichromate have been determined by means of the presented methods and FIA responses that are available in literature. The best-fit results agree with each other and with experimental data thus validating both presented approaches. Copyright © 2013 The Author. Published by Elsevier B.V. All rights reserved.

A microchip-based flow injection-amperometry system with mercaptopropionic acid modified electroless gold microelectrode for the selective determination of dopamine.

PubMed

Wang, Yi; Luo, Jie; Chen, Hengwu; He, Qiaohong; Gan, Nin; Li, Tianhua

2008-09-12

A novel chip-based flow injection analysis (FIA) system has been developed for automatic, rapid and selective determination of dopamine (DA) in the presence of ascorbic acid (AA). The system is composed of a polycarbonate (PC) microfluidic chip with an electrochemical detector (ED), a gravity pump, and an automatic sample loading and injection unit. The selectivity of the ED was improved by modification of the gold working microelectrode, which was fabricated on the PC chip by UV-directed electroless gold plating, with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA). Postplating treatment methods for cleaning the surface of electroless gold microelectrodes were investigated to ensure the formation of high quality SAMs. The effects of detection potential, flow rate, and sampling volume on the performance of the chip-based FIA system were studied. Under optimum conditions, a detection limit of 74 nmol L(-1) for DA was achieved at the sample throughput rate of 180 h(-1). A RSD of 0.9% for peak heights was observed for 19 runs of a 100 micromol L(-1) DA solution. Interference-free determination of DA could be conducted if the concentration ratio of AA-DA was no more than 10.

Elicitation of macrophages from the peritoneal cavity of channel catfish

USGS Publications Warehouse

Jenkins, J.A.; Klesius, P.H.

1998-01-01

Four chemicals were evaluated for elicitation of macrophages in peritoneal cavities of 250-300g healthy channel catfish Ictalurus punctatus. Cellular exudates were collected at 3, 5, 7, 10, 14, and 20 d following intraperitoneal injections with squalene, Freund's incomplete adjuvant (FIA), goat serum, thioglycollate, or as a control, phosphate-buffered saline. Injection with either squalene or FIA induced significantly greater (P ??? 0.0001) macrophage recruitment than the other chemicals. The effectiveness of squalene and FIA was compared further by macrophage collection daily for 7 d. Squalene and FIA elicited similarly high macrophage responses (P ??? 0.0450), the highest being 3.43 x 106 macrophages/mL (SE, 2.4 x l06) at 99% purity at day 2 and 2.1 X 106 macrophages/mL (SE, 0.7 x 106) at day 14 at 80% purity, respectively. In both experiments, the time after injection was not statistically significant, nor was there an interaction between time and chemicals. The occurrence of cells other than macrophages decreased with time to yield macrophage recoveries of 47-99% for squalene and 30-80% for FIA. Two subsets of macrophages were observed by means of flow cytometry. As demonstrated by chemiluminescence, the squalene-elicited cells produced high-energy oxygen compounds important to the phagocytic process.

Design and integration of a generic disposable array-compatible sensor housing into an integrated disposable indirect microfluidic flow injection analysis system.

PubMed

Rapp, Bastian E; Schickling, Benjamin; Prokop, Jürgen; Piotter, Volker; Rapp, Michael; Länge, Kerstin

2011-10-01

We describe an integration strategy for arbitrary sensors intended to be used as biosensors in biomedical or bioanalytical applications. For such devices ease of handling (by a potential end user) as well as strict disposable usage are of importance. Firstly we describe a generic array compatible polymer sensor housing with an effective sample volume of 1.55 μl. This housing leaves the sensitive surface of the sensor accessible for the application of biosensing layers even after the embedding. In a second step we show how this sensor housing can be used in combination with a passive disposable microfluidic chip to set up arbitrary 8-fold sensor arrays and how such a system can be complemented with an indirect microfluidic flow injection analysis (FIA) system. This system is designed in a way that it strictly separates between disposable and reusable components- by introducing tetradecane as an intermediate liquid. This results in a sensor system compatible with the demands of most biomedical applications. Comparative measurements between a classical macroscopic FIA system and this integrated indirect microfluidic system are presented. We use a surface acoustic wave (SAW) sensor as an exemplary detector in this work.

Development of enantioselective chemiluminescence flow- and sequential-injection immunoassays for alpha-amino acids.

PubMed

Silvaieh, Hossein; Schmid, Martin G; Hofstetter, Oliver; Schurig, Volker; Gübitz, Gerald

2002-01-01

The development of an enantioselective flow-through chemiluminescence immunosensor for amino acids is described. The approach is based on a competitive assay using enantioselective antibodies. Two different instrumental approaches, a flow-injection (FIA) and a sequential-injection system (SIA), are used. Compared to the flow-injection technique, the sequential injection-mode showed better repeatability. Both systems use an immunoreactor consisting of a flow cell packed with immobilized haptens. The haptens (4-amino-L- or D-phenylalanine) are immobilized onto a hydroxysuccinimide-activated polymer (Affi-prep 10) via a tyramine spacer. Stereoselective antibodies, raised against 4-amino-L- or D-phenylalanine, are labeled with an acridinium ester. Stereoselective inhibition of binding of the acridinum-labeled antibodies to the immobilized hapten by amino acids takes place. Chiral recognition was observed not only for the hapten molecule but also for a series of different amino acids. One assay cycle including regeneration takes 6:30 min in the FIA mode and 4:40 min in the SIA mode. Using D-phenylalanine as a sample, the detection limit was found to be 6.13 pmol/ml (1.01 ng/ml) for the flow-injection immunoassay (FIIA) and 1.76 pmol/ml (0.29 ng/ml ) for the sequential-injection immunoassay (SIIA) which can be lowered to 0.22 pmol/ml (0.036 ng/ml) or 0.064 pmol/ml (0.01 ng/ml) by using a stopped flow system. The intra-assay repeatability was found to be about 5% RSD and the inter-assay repeatability below 6% (within 3 days).

A novel approach for determination of free fatty acids in vegetable oils by a flow injection system with manual injection.

PubMed

Ayyildiz, H Filiz; Kara, Huseyin; Sherazi, S T H

2011-12-01

A non-aqueous flow injection method for determining free fatty acid (FFA) content in corn and sunflower oil samples was developed. A single-line manifold system was built by modification of an HPLC for flow injection analysis (FIA). Without pre-treatment, oil samples were injected into a n-propanol solution containing KOH and phenolphthalein (PHP). The main parameters, such as flow rate of carrier phase, length, geometry, inner diameters of the coils and reagent concentration were all optimized. The proposed FIA method was validated for precision, accuracy, linear region, limit of detection (LOD) and limit of quantification (LOQ). The intra- and inter-day measurements of the precision of the method were found to be within the limits of acceptance criteria (RSD < 1%), and were rugged when the method was performed by a different analyst. The linear concentration range was calculated as 0.09-1.50 and 0.07-1.40 FFA% for corn and sunflower oils, correspondingly. The LOD and LOQ were found to be 7.53 × 10(-4)-2.28 × 10(-3) oleic acid % and 7.11 × 10(-4)-2.23 × 10(-3) oleic acid % for corn and sunflower oils, respectively. The results were compared with those obtained by the AOCS (Ca-5a-40) method using statistical t and F tests, and a significant difference was not observed between the methods at a 95% confidence level. The proposed method is suitable for quality control of routine applications due to its simplicity, high sample throughput, and economy of solvents and sample, offering considerable promise as a low cost analytical system that needs minimum human intervention over long periods of time.

3D-printed and CNC milled flow-cells for chemiluminescence detection.

PubMed

Spilstead, Kara B; Learey, Jessica J; Doeven, Egan H; Barbante, Gregory J; Mohr, Stephan; Barnett, Neil W; Terry, Jessica M; Hall, Robynne M; Francis, Paul S

2014-08-01

Herein we explore modern fabrication techniques for the development of chemiluminescence detection flow-cells with features not attainable using the traditional coiled tubing approach. This includes the first 3D-printed chemiluminescence flow-cells, and a milled flow-cell designed to split the analyte stream into two separate detection zones within the same polymer chip. The flow-cells are compared to conventional detection systems using flow injection analysis (FIA) and high performance liquid chromatography (HPLC), with the fast chemiluminescence reactions of an acidic potassium permanganate reagent with morphine and a series of adrenergic phenolic amines. Copyright © 2014 Elsevier B.V. All rights reserved.

Development of a reagentless electrochemiluminescent electrode for flow injection analysis using copolymerised luminol/aniline on nano-TiO2 functionalised indium-tin oxide glass.

PubMed

Liu, Chao; Wei, Xiuhua; Tu, Yifeng

2013-07-15

In this study, a nano-structured copolymer of luminol/aniline (PLA) was deposited onto nano-TiO2-functionalised indium tin oxide (ITO)-coated glass by electrochemical polymerisation using cyclic voltammetry (CV). The resulting reagentless electrochemiluminescent (ECL) electrode (ECLode) can be used for flow injection analysis (FIA). The properties of the ECLode were characterised by CV, electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). The ECLode has high background ECL emission as well as excellent stability and reproducibility, and yielding sensitive response towards target analytes. The ECL emissions of the ECLode were 50 times higher than PLA/ITO, and 500 times higher than polyluminol (PL)/ITO. The ECLode showed sensitive responses to reactive oxygen species (ROSs), permitting its application for determination of antioxidants by quenching. Under optimised conditions, an absolute detection limit of 69.9 pg was obtained for resveratrol, comparable to the highest levels of sensitivity achieved by other methods. Thus, the gross antioxidant content of red wine was determined, with satisfactory recoveries between 87.6% and 108.3%. These results suggest a bright future for the use of the ECLode for single-channel FIA due to its high sensitivity, accuracy and reproducibility. Copyright © 2013 Elsevier B.V. All rights reserved.

Novel PVC-membrane electrode for flow injection potentiometric determination of Biperiden in pharmaceutical preparations.

PubMed

Khaled, Elmorsy; El-Sabbagh, Inas A; El-Kholy, N G; Ghahni, E Y Abdel

2011-12-15

The construction and performance characteristics of Biperiden (BP) polyvinyl chloride (PVC) electrodes are described. Different methods for electrode fabrication are tested including; incorporation of BP-ion pairs (BP-IPs), incorporation of ion pairing agents, or soaking the plain electrode in BP-ion pairs suspension solution. Electrode matrices were optimized referring to the effect of modifier content and nature, plasticizer and the method of modification. The proposed electrodes work satisfactorily in the BP concentration range from 10(-5) to 10(-2)mol L(-1), with fast response time (7s) and adequate operational lifetime (28 days). The electrode potential is pH independent within the range 2.0-7.0, with good selectivity towards BP in presence of various interfering species. The developed electrodes have been applied for potentiometric determination of BP in pharmaceutical formulation under batch and flow injection analysis (FIA) conditions. FIA offers the advantages of accuracy and automation feasibility with high sampling frequency. The dissolution profile for Akineton tablets (2mg BP/tablet) was studied using the proposed electrode in comparison with the official methods. Copyright © 2011 Elsevier B.V. All rights reserved.

Flow-injection amperometric determination of glucose using a biosensor based on immobilization of glucose oxidase onto Au seeds decorated on core Fe₃O₄ nanoparticles.

PubMed

Samphao, Anchalee; Butmee, Preeyanut; Jitcharoen, Juthamas; Švorc, Ľubomír; Raber, Georg; Kalcher, Kurt

2015-09-01

An amperometric biosensor based on chemisorption of glucose oxidase (GOx) on Au seeds decorated on magnetic core Fe3O4 nanoparticles (Fe3O4@Au) and their immobilization on screen-printed carbon electrode bulk-modified with manganese oxide (SPCE{MnO2}) was designed for the determination of glucose. The Fe3O4@Au/GOx modified SPCE{MnO2} was used in a flow-injection analysis (FIA) arrangement. The experimental conditions were investigated in amperometric mode with the following optimized parameters: flow rate 1.7 mL min(-1), applied potential +0.38 V, phosphate buffer solution (PBS; 0.1 mol L(-1), pH 7.0) as carrier and 3.89 unit mm(-2) enzyme glucose oxidase loading on the active surface of the SPCE. The designed biosensor in FIA arrangement yielded a linear dynamic range for glucose from 0.2 to 9.0 mmol L(-1) with a sensitivity of 2.52 µA mM(-1) cm(-2), a detection limit of 0.1 mmol L(-1) and a quantification limit of 0.3 mmol L(-1). Moreover, a good repeatability of 2.8% (number of measurements n=10) and a sufficient reproducibility of 4.0% (number of sensors n=3) were achieved. It was found that the studied system Fe3O4@Au facilitated not only a simpler enzyme immobilization but also provided wider linear range. The practical application of the proposed biosensor for FIA quantification of glucose was tested in glucose sirup samples, honeys and energy drinks with the results in good accordance with those obtained by an optical glucose meter and with the contents declared by the producers. Copyright © 2015. Published by Elsevier B.V.

Flow-injection assay of catalase activity.

PubMed

Ukeda, Hiroyuki; Adachi, Yukiko; Sawamura, Masayoshi

2004-03-01

A novel flow-injection assay (FIA) system with a double line for catalase activity was constructed in which an oxidase is immobilized and the substrate is continuously pumped to reduce the dissolved oxygen and to generate a given level of hydrogen peroxide. The catalase in a sample decomposed the hydrogen peroxide, and thus the increase in dissolved oxygen dependent on the activity was amperometrically monitored using a Clark-type oxygen electrode. Among the examined several oxidases, uricase was most suitable for the continuous formation of hydrogen peroxide from a consideration of the stability and the conversion efficiency. Under the optimum conditions, a linear calibration curve was obtained in the range from 21 to 210 units/mg and the reproducibility (CV) was better than 2% by 35 successive determinations of 210 units/ml catalase preparation. The sampling frequency was about 15 samples/h. The present FIA system was applicable to monitor the inactivation of catalase by glycation.

[Determination of aluminum in sediments by atomic absorption spectrophotometer without FIA spectrophotometric analysis].

PubMed

Zhao, Zhen-yi; Han, Guang-xi; Song, Xi-ming; Luo, Zhi-xiong

2008-06-01

To search for a new method of determining, we developed a new flow injection analyzer, applied to the atomic absorption spectrophotometer, relying on it without flame in place of visible spectrophotometer, and studied the appropriate condition for the determination of aluminum in sediments, thus built up a kind of new analytical test technique. Three peak and two valley absorption values (A1, A2, A3, A4 and A5) can be continuously obtained simultaneously that all can be used for quantitative analysis, then we discussed its theory and experiment technique. Based on the additivity of absorbance (A = A1+A2+A3+A4+ A5), the sensitivity of FIA is enhanced, and its precision and linear relation are also good, raising the efficiency of AAS. The simple method has been applied to determining Al in sediments, and the results are satisfactory.

Flow injection spectrophotometric determination of V(V) involving on-line separation using a poly(vinylidene fluoride-co-hexafluoropropylene)-based polymer inclusion membrane.

PubMed

Yaftian, Mohammad Reza; Almeida, M Inês G S; Cattrall, Robert W; Kolev, Spas D

2018-05-01

A poly(vinylidene fluoride-co-hexafluoropropylene)-based polymer inclusion membrane (PIM) using Cyphos® IL 101 (i.e. trihexyl(tetradecyl)phosphonium chloride) as the carrier and 2-nitrophenyl octyl ether as a plasticizer in a mass ratio of 55/35/10 was employed for the on-line extractive separation of V(V) prior to its spectrophotometric determination in a flow injection analysis (FIA) system using xylenol orange as the colorimetric reagent. The selectivity of the membrane allowed the determination of V(V) in sulfate solutions in the presence of a variety of cations and anions. The interference of molybdenum(VI) was eliminated by off-line extraction using the same PIM. A univariate sequential optimization of the newly developed FIA system was conducted and under optimal conditions the system is characterized by a linear concentration range of 0.5-8.0mgL -1 , detection limit of 0.08mgL -1 and sample throughput of 4h -1 . The relative standard deviation at the 3mgL -1 level of V(V) was 2.9% based on 8 replicate determinations. The membrane was stable, which was reflected by the standard deviation value for determinations over three consecutive days (24 determinations of 3mgL -1 V(V)) of 3.6%. The newly developed FIA system was applied to the determination of V(V) in water and dietary supplements samples and a good agreement with inductively coupled plasma optical emission spectrometry was observed. Copyright © 2018 Elsevier B.V. All rights reserved.

Determination of glutamine and glutamic acid in mammalian cell cultures using tetrathiafulvalene modified enzyme electrodes.

PubMed

Mulchandani, A; Bassi, A S

1996-01-01

Tetrathiafulvalene (TTF) mediated amperometric enzyme electrodes have been developed for the monitoring of L-glutamine and L-glutamic acid in growing mammalian cell cultures. The detection of glutamine was accomplished by a coupled enzyme system comprised of glutaminase plus glutamate oxidase, while the detection of glutamic acid was carried out by a single enzyme, glutamate oxidase. The appropriate enzyme(s) were immoblized on the Triton-X treated surface of tetrathiafulvalene modified carbon paste electrodes by adsorption, in conjunction with entrapment by an electrochemically deposited copolymer film of 1,3-phenylenediamine and resorcinol. Operating conditions for the glutamine enzyme electrode were optimized with respect to the amount of enzymes immoblized, pH, temperature and mobile phase flow rate for operation in a flow injection (FIA) system. When applied to glutamine and glutamic acid measurements in mammalian cell culture in FIA, the results obtained with enzyme electrodes were in excellent agreement with those determined by enzymatic analysis.

pH-based fiber optic biosensors for use in clinical and biotechnological applications

NASA Astrophysics Data System (ADS)

Mueller, Cord; Hitzmann, Bernd; Schubert, Florian; Scheper, Thomas

1995-05-01

The development of pH-based fiber optic biosensors and their uses in clinical and biotechnological applications are described. Based on a pH-sensitive optode, different biosensors for urea, penicillin, glucose and creatinine were developed. A multichannel modular fluorimeter was used to measure signals from up to three optodes simultaneously. The pH value and the buffer capacity are critical factors for biosensors based on pH probes and influence the biosensor signal. A flow injection analysis (FIA) system is used to eliminate the latter influences. With this integrated system, samples can be analyzed sequentially by the injection of a defined volume of each sample into a continuously flowing buffer stream that transports the samples to the sensors. The complex signal is transformed and analyzed by a computer system. Characteristic features of the FIA peak give information about the buffer capacity in the solution. With the help of intelligent computing (neural networks) it is possible to recognize these features and relate them to the respective buffer capacity to obtain more accurate values. Various applications of these biosensors are discussed. The pH optode is also used to monitor enzymatic reactions in non aqueous solvents. In this case the production of acetic acid can be detected on line.

Manual and Flow-Injection Detection/Quantification of Polyquaterniums via Fully Reversible Polyion-Sensitive Polymeric Membrane-Based Ion-Selective Electrodes.

PubMed

Ferguson, Stephen A; Meyerhoff, Mark E

2017-10-27

The detection of four different polyquaterniums (PQs) using a fully reversible potentiometric polyion sensor in three different detection modes is described. The polyion sensing "pulstrodes" serve as the detector for direct dose-response experiments, beaker titrations, and in a flow-injection analysis (FIA) system. Direct polycation response toward PQ-2, PQ-6, PQ-10, and poly(2-methacryloxyethyltrimethylammonium) chloride (PMETAC) yields characteristic information about each PQ species (e.g., relative charge densities, etc.) via syringe pump addition of each PQ species to a background electrolyte solution. Quantitative titrations are performed using a syringe pump to deliver heparin as the polyanion titrant to quantify all four PQs at μg/mL levels. Both the direct and indirect methods incorporate the use of a three-electrode system including counter, double junction reference, and working electrodes. The working electrode possesses a plasticized poly(vinyl chloride) (PVC) membrane containing the neutral lipophilic salt of dinonylnaphthalenesulfonate (DNNS - ) tridodecylmethylammonium (TDMA + ). Further, the titration method is shown to be useful to quantify PQ-6 levels in recreational swimming pool water collected in Ann Arbor, MI. Finally, a FIA system equipped with a pulstrode detector is used to demonstrate the ability to potentially quantify PQ levels via a more streamlined and semiautomated testing platform.

Ionophore-Based Potentiometric Sensors for the Flow-Injection Determination of Promethazine Hydrochloride in Pharmaceutical Formulations and Human Urine

PubMed Central

Hassan, Ahmed Khudhair; Saad, Bahruddin; Ghani, Sulaiman Ab; Adnan, Rohana; Rahim, Afidah Abdul; Ahmad, Norariza; Mokhtar, Marina; Ameen, Suham Tawfiq; Al-Araji, Suad Mustafa

2011-01-01

Plasticised poly(vinyl chloride)-based membranes containing the ionophores (α-, β- and γ-cyclodextrins (CD), dibenzo-18-crown-6 (DB18C6) and dibenzo-30-crown-10 (DB30C10) were evaluated for their potentiometric response towards promethazine (PM) in a flow injection analysis (FIA) set-up. Good responses were obtained when β- and γ-CDs, and DB30C10 were used. The performance characteristics were further improved when tetrakis(4-chlorophenyl) borate (KTPB) was added to the membrane. The sensor based on β-CD, bis(2-ethylhexyl) adipate (BEHA) and KTPB exhibited the best performance among the eighteen sensor compositions that were tested. The response was linear from 1 × 10−5 to 1 × 10−2 M, slope was 61.3 mV decade−1, the pH independent region ranged from 4.5 to 7.0, a limit of detection of 5.3 × 10−6 M was possible and a lifetime of more than a month was observed when used in the FIA system. Other plasticisers such as dioctyl phenylphosphonate and tributyl phosphate do not show significant improvements in the quality of the sensors. The promising sensors were further tested for the effects of foreign ions (Li+, Na+, K+, Mg2+, Ca2+, Co2+, Cu2+, Cr3+, Fe3+, glucose, fructose). FIA conditions (e.g., effects of flow rate, injection volume, pH of the carrier stream) were also studied when the best sensor was used (based on β-CD). The sensor was applied to the determination of PM in four pharmaceutical preparations and human urine that were spiked with different levels of PM. Good agreement between the sensor and the manufacturer’s claimed values (for pharmaceutical preparations) was obtained, while mean recoveries of 98.6% were obtained for spiked urine samples. The molecular recognition features of the sensors as revealed by molecular modelling were rationalised by the nature of the interactions and complexation energies between the host and guest molecules. PMID:22346617

Electrochemical biotin detection based on magnetic beads and a new magnetic flow cell for screen printed electrode.

PubMed

Biscay, Julien; González García, María Begoña; Costa García, Agustín

2015-01-01

The use of the first flow-cell for magnetic assays with an integrated magnet is reported here. The flow injection analysis system (FIA) is used for biotin determination. The reaction scheme is based on a one step competitive assay between free biotin and biotin labeled with horseradish peroxidase (B-HRP). The mixture of magnetic beads modified with streptavidin (Strep-MB), biotin and B-HRP is left 15 min under stirring and then a washing step is performed. After that, 100 μL of the mixture is injected and after 30s 100 μL of 3,3',5,5'-Tetramethylbenzidine (TMB) is injected and the FIAgram is recorded applying a potential of -0.2V. The linear range obtained is from 0.01 to 1 nM of biotin and the sensitivity is 758 nA/nM. The modification and cleaning of the electrode are performed in an easy way due to the internal magnet of the flow cell. Copyright © 2014 Elsevier B.V. All rights reserved.

Incorporation of flow injection analysis with dual-wavelength overlapping resonance Rayleigh scattering for rapid determination of malachite green and its metabolite in fish.

PubMed

Zhu, Jinghui; Qin, Mingyou; Liu, Shaopu; Liu, Zhongfang; Yang, Jidong; Hu, Xiaoli

2014-09-15

A flow injection analysis (FIA) system combined with dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) has been established and validated for rapid determination of malachite green (MG) and its metabolite in fish samples. Under experimental condition, MG would react with Erythrosin (Ery) to form ion-association complexes, resulting in the occurrence of two RRS peaks and a dramatic enhancement of RRS intensity. The maximum RRS peaks were located at 286 nm and 337 nm. It is noted that the increments of both of these two peaks were proportional to the concentration of MG. The detection limit of DWO-RRS was 1.5 ng/mL, which was comparable to several reported methods. Moreover, the results of real sample analysis exhibited an acceptable recovery between 97.5% and 103.6%, indicating that the method had good reproducibility. Copyright © 2014 Elsevier B.V. All rights reserved.

Determination of the titratable acidity and the pH of wine based on potentiometric flow injection analysis.

PubMed

Vahl, Katja; Kahlert, Heike; von Mühlen, Lisandro; Albrecht, Anja; Meyer, Gabriele; Behnert, Jürgen

2013-07-15

A FIA system using a pH-sensitive detector based on a graphite/quinhydrone/silicone composite electrode was applied to determine sequentially the titratable acidity and the pH of wine, as well as the sum of calcium and magnesium ions. For all measurements the same FIA configuration was used employing different carrier solutions. The results for the determination of acidity and pH are in good agreement with those obtained by classical potentiometric titrations and by pH measurements using a conventional glass electrode. The standard deviation was less than 1.5% for both kinds of measurements and the sample volume was 150 μL. The method allows about 40 determinations of titratable acidity per hour and 30 pH measurements per hour. The titration method can be adjusted to the legal requirements in USA and Europe. Copyright © 2013 Elsevier B.V. All rights reserved.

Development and characterization of a FIA system for selective assay of L-ascorbic acid in food samples.

PubMed

Vig, Attila; Igloi, Attila; Adanyi, Nora; Gyemant, Gyongyi; Csutoras, Csaba; Kiss, Attila

2010-10-01

An amperometric detector and an enzymatic reaction were combined for the measurement of L-ascorbic acid. The enzyme cell (containing immobilized ascorbate oxidase) was connected to a flow injection analyzer (FIA) system with a glassy carbon electrode as an amperometric detector. During optimization and measurements two sample injectors were used, one before and one after the enzyme cell, thus eliminating the background interferences. Subtraction of the signal area given in the presence of enzyme from the one given in the absence of enzyme was applied for measuring analyte concentrations and calibration at 400 mV. Analysis capacity of system is 25 samples/hour. The relative standard deviation (RSD) was below 5% (5 times repeated, 400 μmol/L conc.), linearity up to 400 μmol/L, limit of detection (LOD) 5 μmol/L, fitting of calibration curve in 25-400 μmol/L range was R (2) = 0.99.

Amperometric Glucose Sensor Using Thermostable Co-Factor Binding Glucose Dehydrogenase

NASA Astrophysics Data System (ADS)

Nakazawa, Yukie; Yamazaki, Tomohiko; Tsugawa, Wakako; Ikebukuro, Kazunori; Sode, Koji

A thermostable mediator-type enzyme glucose sensor was constructed. The electrode was fabricated using chemically cross-linked thermostable co-factor binding glucose dehydrogenase (GDH) from thermophilic bacteria in carbon paste matrix. The electrode responded directly proportional to D-glucose concentration from 0.01 mM to 3 mM in stirred buffer containing 1 mM 1-methoxyphenazinemethosulfate as a mediator with the steady-state mode. The storage stability was examined by incubating the enzyme electrode at 50oC during the measurement. The cross-linked GDH immobilized electrode showed good storage stability. Ninety percent of its initial response was retained after incubation in buffer solution for 9 days at 50oC. The flow injection analysis (FIA) glucose sensing system was also constructed by immobilizing the cross-linked GDH and ferrocene as a mediator in the carbon paste matrix. The FIA system was able to measure 600 samples for 100 h.

Mid-infrared quantum cascade lasers for flow injection analysis

PubMed

Lendl; Frank; Schindler; Muller; Beck; Faist

2000-04-01

A Fabry-Perot quantum cascade laser (QCL) was used as a powerful light source for mid infrared (MIR) detection in flow injection analysis. The QCL lased at several wavelengths close to each other within a few wavenumbers (990-1010 cm-1), hence fitting well to the broad absorption bands of molecules in liquid phase. As compared with that of a state-of-the-art Fourier transform spectrometer, the signal-to-noise ratio could be improved by a factor of 50. Additionally, by using a QCL as the light source, optical path lengths of more than 100 microns could be used even in aqueous matrixes which reduced the danger of cell clogging. In the example shown here phosphate was determined in Diet Coke samples. The flow injection system used allowed the measurement of the sample at two pH values (5 and 13) at which the analyte was present as H2PO4- and PO4(3-), respectively. As the analytical readout the difference in IR absorption of H2PO4- and PO4(3-) at the laser wavelengths was taken. The FIA-QCL measurements were corroborated by ion chromatography which was used as a reference method.

Trends in Flow-based Biosensing Systems for Pesticide Assessment

PubMed Central

Prieto-Simón, Beatriz; Campàs, Mònica; Andreescu, Silvana; Marty, Jean-Louis

2006-01-01

This review gives a survey on the state of the art of pesticide detection using flow-based biosensing systems for sample screening. Although immunosensor systems have been proposed as powerful pesticide monitoring tools, this review is mainly focused on enzyme-based biosensors, as they are the most commonly employed when using a flow system. Among the different detection methods able to be integrated into flow-injection analysis (FIA) systems, the electrochemical ones will be treated in more detail, due to their high sensitivity, simple sample pretreatment, easy operational procedures and real-time detection. During the last decade, new trends have been emerging in order to increase the enzyme stability, the sensitivity and selectivity of the measurements, and to lower the detection limits. These approaches are based on (i) the design of novel matrices for enzyme immobilisation, (ii) new manifold configurations of the FIA system, sometimes including miniaturisation or lab-on-chip protocols thanks to micromachining technology, (iii) the use of cholinesterase enzymes either from various commercial sources or genetically modified with the aim of being more sensitive, (iv) the incorporation of other highly specific enzymes, such as organophosphate hydrolase (OPH) or parathion hydrolase (PH) and (v) the combination of different electrochemical methods of detection. This article discusses these novel strategies and their advantages and limitations.

Flow injection analysis of picric acid explosive using a copper electrode as electrochemical detector.

PubMed

Junqueira, João R C; de Araujo, William R; Salles, Maiara O; Paixão, Thiago R L C

2013-01-30

A simple and fast electrochemical method for quantitative analysis of picric acid explosive (nitro-explosive) based on its electrochemical reduction at copper surfaces is reported. To achieve a higher sample throughput, the electrochemical sensor was adapted in a flow injection system. Under optimal experimental conditions, the peak current response increases linearly with picric acid concentration over the range of 20-300 μmol L(-1). The repeatability of the electrode response in the flow injection analysis (FIA) configuration was evaluated as 3% (n=10), and the detection limit of the method was estimated to be 6.0 μmol L(-1) (S/N=3). The sample throughput under optimised conditions was estimated to be 550 samples h(-1). Peroxide explosives like triacetone triperoxide (TATP) and hexamethylene triperoxide diamine (HMTD) were tested as potential interfering substances for the proposed method, and no significant interference by these explosives was noticed. The proposed method has interesting analytical parameters, environmental applications, and low cost compared with other electroanalytical methods that have been reported for the quantification of picric acid. Additionally, the possibility to develop an in situ device for the detection of picric acid using a disposable sensor was evaluated. Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.

The determination of trace lead in Chinese medicinal herbs by flow injection analysis in polyethyleneglycol medium

NASA Astrophysics Data System (ADS)

Gong, Aiqin; Zhu, Xiashi; Huang, Xiaoyan; Zhang, Yaqin

2008-01-01

In this work, a new flow injection analysis (FIA) for the determination of Pb 2+ in Chinese medicinal herbs was developed. In the buffer solution of borax-NaOH (pH 10.5), Pb 2+ reacted with 2-[(5-bromo-2-pyridyl)-azo]-5-(diethyl-amino)phenol (5-Br-PADAP) to form a complex. The experimental results showed that the sensitivity was enhanced in the presence of polyethyleneglycol-800 (PG-800). The main factors affecting the determination were investigated in detail. Under the optimum conditions, the linear range and detection limit is 0.0-0.3 μg/mL and 1.5 ng/mL (correlation coefficient r = 0.9996), respectively. The linear regression equation is A = -0.005 + 0.60 c (μg/mL). The sample throughout is 10 h -1. Foreign substrates effects were also investigated. The proposed method has been successfully applied to the determination of lead in reference material, goldthread and lepidium seed.

Determination of uranium in clinical and environmental samples by FIAS-ICPMS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karpas, Z.; Lorber, A.; Halicz, L.

Uranium may enter the human body through ingestion or inhalation. Ingestion of uranium compounds through the diet, mainly drinking water, is a common occurrence, as these compounds are present in the biosphere. Inhalation of uranium-containing particles is mainly an occupational safety problem, but may also take place in areas where uranium compounds are abundant. The uranium concentration in urine samples may serve as an indication of the total uranium body content. A method based on flow injection and inductively coupled plasma mass spectrometry (FIAS-ICPMS) was found to be most suitable for determination of uranium in clinical samples (urine and serum),more » environmental samples (seawater, wells and carbonate rocks) and in liquids consumed by humans (drinking water and commercial beverages). Some examples of the application of the FIAS-ICPMS method are reviewed and presented here.« less

A dual-wavelength light-emitting diode based detector for flow-injection analysis process analysers.

PubMed

Huang, J; Liu, H; Tan, A; Xu, J; Zhao, X

1992-06-01

In this paper, a small dual-wavelength light-emitting diode (LED) based detector for FIA process analysers is designed. The detector's optical parts include a flow cell, a dual-wavelength LED and a photodiode. Neither mirrors nor lenses are used. The optical paths for the different light beams are almost the same, distinguishing it from previously reported LED based detectors. The detector's electronic components, including a signal amplifier, an A/D and D/A converter, and an Intel 8031 single-chip microcomputer, are integrated on one small board. In order to obtain response signals of approximate intensity for the two colours, the D/A converter and a multiplexer are used to adjust the emission intensity of the two colours respectively. Under microcomputer control, light beams are rapidly electronically modulated. Therefore, dark current and intensity of the light beams are measured almost simultaneously; as a result, the effect of drift is negligible. While a solution of absorbance 0.875 was measured repeatedly, an RSD (relative standard deviation) of 0.24% could be reached. Furthermore, such a detector with a red/yellow LED has been coupled with the FIA technique for the determination of 10(-6)M levels of cobalt.

Alkyd paints in art: characterization using integrated mass spectrometry.

PubMed

La Nasa, Jacopo; Degano, Ilaria; Modugno, Francesca; Colombini, Maria Perla

2013-10-03

Alkyd resins have been commonly used as binders in artist paints since the 1940s. The characterization of alkyds in samples from artworks can help to solve attribution and dating issues, investigate decay processes, and contribute to the planning of conservation strategies. Being able to assess the components of industrially formulated paint materials and to differentiate between different trademarks and producers is extremely interesting and requires multi-analytical approaches. In this paper we describe the characterization of commercial alkyd paint materials using a multi-analytical approach based on the integration of three different mass spectrometric techniques: gas chromatography-mass spectrometry (GC/MS), high performance liquid chromatography coupled with electrospray ionization mass spectrometry with a tandem quadrupole-time of flight mass spectrometer (HPLC-ESI-Q-ToF), and flow injection analysis (FIA) in the ESI-Q-ToF mass spectrometer. GC/MS was successful in determining the fatty acid and aromatic fractions of the resins after hydrolysis; HPLC-ESI-Q-ToF analysis enabled us to identify the triglycerides (TAGs) and diglycerides (DAGs) profile of each resin, and FIA analysis was used as a rapid method to evaluate the presence of possible additives such as synthetic polymers. Copyright © 2013 Elsevier B.V. All rights reserved.

Novel miniaturized sensors for potentiometric batch and flow-injection analysis (FIA) of perchlorate in fireworks and propellants.

PubMed

Almeer, Saeed H M A; Zogby, Ibrahim A; Hassan, Saad S M

2014-11-01

Three planar miniaturized perchlorate membrane sensors (3×5 mm(2)) are prepared using a flexible Kaptan substrate coated with nitron-perchlorate (NT-ClO4) [sensor 1], methylene blue-perchlorate (MB-ClO4) [sensor II] and indium-porphyrin (In-Por) [sensor III] as electroactive materials in PVC membranes plasticized with 2-NPPE. Sensors I, II and III display near-Nernstian response for 1.0×10(-5)-1.0×10(-2), 3.1×10(-5)-1.0×10(-2) and 3.1×10(-6)-1.0×10(-2) mol L(-1) ClO4(-) with lower detection limits of 6.1×10(-6), 6.9×10(-6) and 1.2×10(-6) mol L(-1), and anionic calibration slopes of 50.9±0.4, 48.4±0.4 and 57.7±0.3 mV decade(-1), respectively. Methods for determining perchlorate using these sensors offer many attractive advantages including simplicity, flexibility, cost effectiveness, wide linear dynamic response range (0.1-1000 ppm), low detection limit (<1.2×10(-6) mol L(-1)≡0.1 ppm), small sample test volume (100 μL), safety, short response time (<20 s), long life span (~8 weeks), and extended wide working pH range (4.5-8.0). The sensors show high selectivity in the presence of some inorganic ions (e.g., PO4(3-), SO4(2-), S2O3(2-), NO2(-), NO3(-), N3(-), CN(-), Cl(-), Br(-), I(-)) and automation feasibility. Indium-porphyrin based membrane sensor (sensor III) is used as a detector in a wall-jet flow injection set-up to enable accurate flow injection analysis (FIA) of perchlorate in some fireworks without interferences from the associated reducing agents (sulfur and charcoal), binders (dextrin, lactose), coloring agents (calcium, strontium, copper, iron, sodium), color brighten (linseed oil) and regulators (aluminum flakes) which are commonly used in the formulations. The sensor is also used for perchlorate assessment in some propellant powders. The results fairly agree with data obtained by ion-chromatography. Copyright © 2014 Elsevier B.V. All rights reserved.

Preliminary evaluation of monolithic column high-performance liquid chromatography with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection for the determination of quetiapine in human body fluids.

PubMed

Bellomarino, Sara A; Brown, Allyson J; Conlan, Xavier A; Barnett, Neil W

2009-03-15

High-performance liquid chromatography (HPLC) with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection methodology is reported for the determination of the atypical antipsychotic drug quetiapine and the observation of its major active and inactive metabolites in human urine and serum. The method uses a monolithic chromatographic column allowing high flow rates of 3 mLmin(-1) enabling rapid quantification. Flow injection analysis (FIA) with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection and HPLC time of flight mass spectrometry (TOF-MS) were used for the determination of quetiapine in a pharmaceutical preparation to establish its suitability as a calibration standard. The limit of detection achieved with FIA was 2 x 10(-11) molL(-1) in simple aqueous solution. The limits of detection achieved with HPLC were 7 x 10(-8) and 2 x 10(-10) molL(-1) in urine and serum, respectively. The calibration range for FIA was between 5 x 10(-9) and 1 x 10(-6) molL(-1). The calibration ranges for HPLC were between 1 x 10(-7)-1 x 10(-4) and 1 x 10(-8)-1 x 10(-4) molL(-1) in urine and serum, respectively. The quetiapine concentrations in patient samples were found to be 3 x 10(-6) molL(-1) in urine and 7 x 10(-7) molL(-1) in serum. Without the need for preconcentration, the HPLC detection limits compared favourably with those in previously published methodologies. The metabolites were identified using HPLC-TOF-MS.

A Review of Flow Analysis Methods for Determination of Radionuclides in Nuclear Wastes and Nuclear Reactor Coolants

DOE PAGES

Trojanowicz, Marek; Kolacinska, Kamila; Grate, Jay W.

2018-02-13

Here, the safety and security of nuclear power plant operations depend on the application of the most appropriate techniques and methods of chemical analysis, where modern flow analysis methods prevail. Nevertheless, the current status of the development of these methods is more limited than it might be expected based on their genuine advantages. The main aim of this paper is to review the automated flow analysis procedures developed with various detection methods for the nuclear energy industry. The flow analysis methods for the determination of radionuclides, that have been reported to date, are primarily focused on their environmental applications. Themore » benefits of the application of flow methods in both monitoring of the nuclear wastes and process analysis of the primary circuit coolants of light water nuclear reactors will also be discussed. The application of either continuous flow methods (CFA) or injection methods (FIA, SIA) of the flow analysis with the β–radiometric detection shortens the analysis time and improves the precision of determination due to mechanization of certain time-consuming operations of the sample processing. Compared to the radiometric detection, the mass spectrometry (MS) detection enables one to perform multicomponent analyses as well as the determination of transuranic isotopes with much better limits of detection.« less

A Review of Flow Analysis Methods for Determination of Radionuclides in Nuclear Wastes and Nuclear Reactor Coolants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Trojanowicz, Marek; Kolacinska, Kamila; Grate, Jay W.

Here, the safety and security of nuclear power plant operations depend on the application of the most appropriate techniques and methods of chemical analysis, where modern flow analysis methods prevail. Nevertheless, the current status of the development of these methods is more limited than it might be expected based on their genuine advantages. The main aim of this paper is to review the automated flow analysis procedures developed with various detection methods for the nuclear energy industry. The flow analysis methods for the determination of radionuclides, that have been reported to date, are primarily focused on their environmental applications. Themore » benefits of the application of flow methods in both monitoring of the nuclear wastes and process analysis of the primary circuit coolants of light water nuclear reactors will also be discussed. The application of either continuous flow methods (CFA) or injection methods (FIA, SIA) of the flow analysis with the β–radiometric detection shortens the analysis time and improves the precision of determination due to mechanization of certain time-consuming operations of the sample processing. Compared to the radiometric detection, the mass spectrometry (MS) detection enables one to perform multicomponent analyses as well as the determination of transuranic isotopes with much better limits of detection.« less

A review of flow analysis methods for determination of radionuclides in nuclear wastes and nuclear reactor coolants.

PubMed

Trojanowicz, Marek; Kołacińska, Kamila; Grate, Jay W

2018-06-01

The safety and security of nuclear power plant operations depend on the application of the most appropriate techniques and methods of chemical analysis, where modern flow analysis methods prevail. Nevertheless, the current status of the development of these methods is more limited than it might be expected based on their genuine advantages. The main aim of this paper is to review the automated flow analysis procedures developed with various detection methods for the nuclear energy industry. The flow analysis methods for the determination of radionuclides, that have been reported to date, are primarily focused on their environmental applications. The benefits of the application of flow methods in both monitoring of the nuclear wastes and process analysis of the primary circuit coolants of light water nuclear reactors will also be discussed. The application of either continuous flow methods (CFA) or injection methods (FIA, SIA) of the flow analysis with the β-radiometric detection shortens the analysis time and improves the precision of determination due to mechanization of certain time-consuming operations of the sample processing. Compared to the radiometric detection, the mass spectrometry (MS) detection enables one to perform multicomponent analyses as well as the determination of transuranic isotopes with much better limits of detection. Copyright © 2018 Elsevier B.V. All rights reserved.

Flow injection analysis of nitrate and nitrite in commercial baby foods.

PubMed

Chetty, Adrian A; Prasad, Surendra

2016-04-15

Commercial baby foods are an easy alternative to home-made meals especially for working parents in a nuclear family therefore it is imperative to determine the nitrate and nitrite content in commercially available baby foods varieties marketed in Fiji. A total of 108 baby food samples were analyzed for nitrate and nitrite using our standardized flow injection analysis (FIA) technique with colorimetric detection technique employing sulfanilamide and N-(1-naphthyl)ethylenediamine dihydrochloride as color reagents where the samples throughput was 38 h(-1). The commercial baby food varieties chosen comprised of vegetables, cereals, fruits and milk. The study shows that the nitrate content of the baby foods studied ranges from 2.10 to 220.67 mg kg(-1) whereas the nitrite content ranges from 0.44 to 3.67 mg kg(-1). Typical recoveries of spiked nitrate residues ranged from 92% to 106%. The study shows that the average nitrate content of commercially available baby foods in Fiji descends below the maximum level proposed by the European Union Legislation. Copyright © 2015 Elsevier Ltd. All rights reserved.

Novel Shear-horizontal Surface Acoustic Wave Based Immunosensors Using SiO2Waveguiding Layers And Flow Injection Analysis.

PubMed

Guo, X S; Chen, Y Q; Yang, X L; Wang, L R

2005-01-01

Surface acoustic wave (SAW) devices based on shear-horizontal (SH) waves can be used as mass-sensitive immunosensors. This paper presents a novel SH-SAW sensor to detect anti-immunoglobulin (IgG) molecules by means of the antibody-antigen binding mechanism. The sensor system comprising dual delay lines was fabricated on 36° Y-X LiTaO3substrate. A SiO2layer was used as love mode waveguiding layers, well as insulating and chemically resistant protective layer. Moreover, flow injection analysis (FIA) method was used for continuous detection the protein molecules. The protein A was immobilized on the optional surface of the gold layer, then coupled with IgG to adsorb the antigens to be measured in the protein solution. The operational frequency of the system changed due to the interaction of antibody-antigen binding. The experimental result demonstrates the sensor has stable frequency response to the mass loading effect of the various anti-IgG concentrations with the sensitivity up to 3.3ng/ml/Hz.

Continuous and selective measurement of oxytocin and vasopressin using boron-doped diamond electrodes

NASA Astrophysics Data System (ADS)

Asai, Kai; Ivandini, Tribidasari A.; Einaga, Yasuaki

2016-09-01

The electrochemical detection of oxytocin using boron-doped diamond (BDD) electrodes was studied. Cyclic voltammetry of oxytocin in a phosphate buffer solution exhibits an oxidation peak at +0.7 V (vs. Ag/AgCl), which is attributable to oxidation of the phenolic group in the tyrosyl moiety. Furthermore, the linearity of the current peaks obtained in flow injection analysis (FIA) using BDD microelectrodes over the oxytocin concentration range from 0.1 to 10.0 μM with a detection limit of 50 nM (S/N = 3) was high (R2 = 0.995). Although the voltammograms of oxytocin and vasopressin observed with an as-deposited BDD electrode, as well as with a cathodically-reduced BDD electrode, were similar, a clear distinction was observed with anodically-oxidized BDD electrodes due to the attractive interaction between vasopressin and the oxidized BDD surface. By means of this distinction, selective measurements using chronoamperometry combined with flow injection analysis at an optimized potential were demonstrated, indicating the possibility of making selective in situ or in vivo measurements of oxytocin.

Implementation of a new integrated d-lactic acid biosensor in a semiautomatic FIA system for the simultaneous determination of lactic acid enantiomers. Application to the analysis of beer samples.

PubMed

Vargas, E; Ruiz, M A; Campuzano, S; González de Rivera, G; López-Colino, F; Reviejo, A J; Pingarrón, J M

2016-05-15

An integrated amperometric d-lactic acid biosensor involving a gold film deposited by sputtering on a stainless steel disk electrode where the enzymes D-lactic acid dehydrogenase (DLDH) and diaphorase (DP) as well as the redox mediator tetrathiafulvalene (TTF) are coimmobilized by using a dialysis membrane, is reported in this work. Amperometry in stirred solutions at a detection potential of +0.15 V (vs Ag/AgCl reference electrode) provided a linear calibration plot for D-lactic acid over the 1.0×10(-4) to 3.8×10(-3) g L(-1) concentration range, with a limit of detection of 3.1×10(-5) g L(-1). The usefulness of the biosensor was demonstrated by determining D-lactic acid in beer samples with good results. Additionally, the biosensor was implemented together with a commercial L-lactic amperometric biosensor in a semiautomatic flow-injection analysis (FIA) system able to perform a rapid and simple stereo-specific determination of D- and D-lactic without a previous separation step. The operational characteristics of the biosensors under flow conditions were evaluated and its applicability was demonstrated through the simultaneous determination of both enantiomers in beer samples. Copyright © 2016 Elsevier B.V. All rights reserved.

Analysis of total polyphenols in wines by FIA with highly stable amperometric detection using carbon nanotube-modified electrodes.

PubMed

Arribas, Alberto Sánchez; Martínez-Fernández, Marta; Moreno, Mónica; Bermejo, Esperanza; Zapardiel, Antonio; Chicharro, Manuel

2013-02-15

The use of glassy carbon electrodes (GCEs) modified with multi-walled carbon nanotube (CNT) films for the continuous monitoring of polyphenols in flow systems has been examined. The performance of these modified electrodes was evaluated and compared to bare GCE by cyclic voltammetry experiments and by flow injection analysis (FIA) with amperometric detection monitoring the response of gallic, caffeic, ferulic and p-coumaric acids in 0.050 M acetate buffer pH 4.5 containing 100 mM NaCl. The GCE modified with CNT dispersions in polyethyleneimine (PEI) provided lower overpotentials, higher sensitivity and much higher signal stability under a dynamic regime than bare GCEs. These properties allowed the estimation of the total polyphenol content in red and white wines with a remarkable long-term stability in the measurements despite the presence of potential fouling substances in the wine matrix. In addition, the versatility of the electrochemical methodology allowed the selective estimation of the easily oxidisable polyphenol fraction as well as the total polyphenol content just by tuning the detection potential at +0.30 or 0.70 V, respectively. The significance of the electrochemical results was demonstrated through correlation studies with the results obtained with conventional spectrophotometric assays for polyphenols (Folin-Ciocalteu, absorbance at 280 nm index and colour intensity index). Copyright © 2012 Elsevier Ltd. All rights reserved.

Multi-reverse flow injection analysis integrated with multi-optical sensor for simultaneous determination of Mn(II), Fe(II), Cu(II) and Fe(III) in natural waters.

PubMed

Youngvises, Napaporn; Suwannasaroj, Kittigan; Jakmunee, Jaroon; AlSuhaimi, Awadh

2017-05-01

Multi-reverse flow injection analysis (Mr-FIA) integrated with multi-optical sensor was developed and optimized for the simultaneous determination of multi ions; Mn(II), Fe(II), Cu(II) and Fe(III) in water samples. The sample/standard solutions were propelled making use of a four channels peristaltic pump whereas 4 colorimetric reagents specific for the metal ions were separately injected in sample streams using multi-syringe pump. The color zones that formed in the individual mixing coils were then streamed into multi-channels spectrometer, which comprised of four flows through cell and four pairs of light emitting diode and photodiode, whereby signals were measured concurrently. The linearity range (along with detection limit, µgL -1 ) was 0.050-3.0(16), 0.30-2.0 (11), 0.050-1.0(12) and 0.10-1.0(50)mgL -1 , for Mn(II), Fe(II), Cu(II) and Fe(III), respectively. In the interim, the correlation coefficients were 0.9924-0.9942. The percentages relative standard deviation was less than 3. The proposed system was applied successfully to determine targeted metal ions simultaneously in natural water with high sample throughput and low reagent consumption, thus it satisfies the criteria of Green Analytical Chemistry (GAC) and its goals. Copyright © 2016 Elsevier B.V. All rights reserved.

Influences of Detection Pinhole and Sample Flow on Thermal Lens Detection in Microfluidic Systems

NASA Astrophysics Data System (ADS)

Liu, Mingqiang; Franko, Mladen

2014-12-01

Thermal lens microscopy (TLM), due to its high temporal () and spatial resolution (), has been coupled to lab-on-chip chemistry for detection of a variety of compounds in chemical or biological fields. Due to the very short optical path length (usually below 100 ) in a microchip, the sensitivity of TLM is unfortunately still 10 to 100 times lower than conventional TLS with 1 cm sample length. Optimization of the TLM optical configuration was made with respect to different pinhole aperture-to-beam size ratios for the best signal-to-noise ratio. In the static mode, the instrumental noise comes mainly from the shot noise of the probe beam when the chopper frequency is over 1 kHz or from the flicker noise of the probe beam at low frequencies. In the flowing mode, the flow-induced noise becomes dominant when the flow rate is high. At a given flow rate, fluids with a higher density and/or a higher viscosity will cause larger flow-induced noise. As an application, a combined microfluidic flow injection analysis ()-TLM device was developed for rapid determination of pollutants by colorimetric reactions. Hexavalent chromium [Cr(VI)] was measured as a model analyte. Analytical signals for 12 sample injections in 1 min have been recorded by the FIA-TLM. For injections of sub-L samples into the microfluidic stream in a deep microchannel, a limit of detection of was achieved for Cr(VI) in water at 60 mW excitation power.

Electrochemical Quartz Crystal Nanobalance (EQCN) Based Biosensor for Sensitive Detection of Antibiotic Residues in Milk.

PubMed

Bhand, Sunil; Mishra, Geetesh K

2017-01-01

An electrochemical quartz crystal nanobalance (EQCN), which provides real-time analysis of dynamic surface events, is a valuable tool for analyzing biomolecular interactions. EQCN biosensors are based on mass-sensitive measurements that can detect small mass changes caused by chemical binding to small piezoelectric crystals. Among the various biosensors, the piezoelectric biosensor is considered one of the most sensitive analytical techniques, capable of detecting antigens at picogram levels. EQCN is an effective monitoring technique for regulation of the antibiotics below the maximum residual limit (MRL). The analysis of antibiotic residues requires high sensitivity, rapidity, reliability and cost effectiveness. For analytical purposes the general approach is to take advantage of the piezoelectric effect by immobilizing a biosensing layer on top of the piezoelectric crystal. The sensing layer usually comprises a biological material such as an antibody, enzymes, or aptamers having high specificity and selectivity for the target molecule to be detected. The biosensing layer is usually functionalized using surface chemistry modifications. When these bio-functionalized quartz crystals are exposed to a particular substance of interest (e.g., a substrate, inhibitor, antigen or protein), binding interaction occurs. This causes a frequency or mass change that can be used to determine the amount of material interacted or bound. EQCN biosensors can easily be automated by using a flow injection analysis (FIA) setup coupled through automated pumps and injection valves. Such FIA-EQCN biosensors have great potential for the detection of different analytes such as antibiotic residues in various matrices such as water, waste water, and milk.

Ion Imprinted Polymer for Preconcentration and Determination of Ultra-Trace Cadmium, Employing Flow Injection Analysis with Thermo Spray Flame Furnace Atomic Absorption Spectrometry.

PubMed

do Lago, Ayla Campos; Marchioni, Camila; Mendes, Tássia Venga; Wisniewski, Célio; Fadini, Pedro Sergio; Luccas, Pedro Orival

2016-11-01

This work proposes a preconcentration method using an ion imprinted polymer (IIP) for determination of cadmium, in several samples, employing a mini-column filled with the polymer coupled into a flow injection analysis system with detection by thermospray flame furnace atomic absorption spectrometry (FIA-TS-FF-AAS). The polymer was synthesized via bulk using methacrylic acid and vinylimidazole as a functional monomer. For the FIA system initial assessment, the variables: pH, eluent concentration and buffer concentration were studied, employing a 23 full factorial design. To obtain the optimum values for each significant variable, a Doehlert matrix was employed. After the optimization conditions as: pH 5.8, eluent (HNO3) concentration of 0.48 mol L -1 and buffer concentration of 0.01 mol L -1 , were adopted. The proposed method showed a linear response in the range of 0.081-10.0 μg L -1 , limits detection and quantification of 0.024 and 0.081 μg L -1 , respectively; preconcentration factor of 165, consumptive index of 0.06 mL, concentration efficiency 132 min -1 , and frequency of readings equal to 26 readings h -1 The accuracy was checked by analysis of certified reference materials for trace metals and recovery tests. The obtained results were in agreement with 95% confidence level (t-test). The method was adequate to apply in samples of: jewelry (earrings) (2.38 ± 0.28 μg kg -1 ), black tea (1.09 ± 0.15 μg kg -1 ), green tea (3.85 ± 0.13 μg kg -1 ), cigarette tobacco (38.27 ± 0.22 μg kg -1 ), and hair (0.35 ± 0.02 μg kg -1 ). © The Author(s) 2016.

Validation of amino-acids measurement in dried blood spot by FIA-MS/MS for PKU management.

PubMed

Bruno, C; Dufour-Rainfray, D; Patin, F; Vourc'h, P; Guilloteau, D; Maillot, F; Labarthe, F; Tardieu, M; Andres, C R; Emond, P; Blasco, H

2016-09-01

Phenylketonuria (PKU) is a metabolic disorder leading to high concentrations of phenylalanine (Phe) and low concentrations of tyrosine (Tyr) in blood and brain that may be neurotoxic. This disease requires a regular monitoring of plasma Phe and Tyr as well as branched-chain amino-acids concentrations to adapt the Phe-restricted diet and other therapy that may be prescribed in PKU. We validated a Flow Injection Analysis tandem Mass Spectrometry (FIA-MS/MS) to replace the enzymatic method routinely used for neonatal screening in order to monitor in parallel to Phe, Tyr and branched-chain amino-acids not detected by the enzymatic method. We ascertained the performances of the method: linearity, detection and quantification limits, contamination index, accuracy. We cross validated the FIA-MS/MS and enzymatic methods and we evaluated our own reference ranges to monitor Phe, Tyr, Leu, Val on 59 dried blood spots of normal controls. We also evaluated Tyr, Leu and Val concentrations in PKU patients to detect some potential abnormalities, not evaluated by the enzymatic method. We developed a rapid method with excellent performances including precision and accuracy <15%. We noted an excellent correlation of Phe concentrations between FIA-MS/MS and enzymatic methods (p<0.0001) based on our database which are similar to references ranges published. We observed that 50% of PKU patients had lower concentrations of Tyr, Leu and/or Val that could not be detected by the enzymatic method. Based on laboratory accreditation recommendations, we validated a robust, rapid and reliable FIA-MS/MS method to monitor plasma Phe concentrations but also Tyr, Leu and Val concentrations, suitable for PKU management. We evaluated our own reference ranges of concentration for a routine application of this method. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Wide-range screening of psychoactive substances by FIA-HRMS: identification strategies.

PubMed

Alechaga, Élida; Moyano, Encarnación; Galceran, Maria Teresa

2015-06-01

Recreational drugs (illicit drugs, human and veterinary medicines, legal highs, etc.) often contain lacing agents and adulterants which are not related to the main active ingredient. Serious side effects and even the death of the consumer have been related to the consumption of mixtures of psychoactive substances and/or adulterants, so it is important to know the actual composition of recreational drugs. In this work, a method based on flow injection analysis (FIA) coupled with high-resolution mass spectrometry (HRMS) is proposed for the fast identification of psychoactive substances in recreational drugs and legal highs. The FIA and HRMS working conditions were optimized in order to detect a wide range of psychoactive compounds. As most of the psychoactive substances are acid-base compounds, methanol-0.1 % aqueous formic acid (1:1 v/v) as a carrier solvent and electrospray in both positive ion mode and negative ion mode were used. Two data acquisition modes, full scan at high mass resolution (HRMS) and data-dependent tandem mass spectrometry (ddMS/HRMS) with a quadrupole-Orbitrap mass analyzer were used, resulting in sufficient selectivity for identification of the components of the samples. A custom-made database containing over 450 substances, including psychoactive compounds and common adulterants, was built to perform a high-throughput target and suspect screening. Moreover, online accurate mass databases and mass fragmenter software were used to identify unknowns. Some examples, selected among the analyzed samples of recreational drugs and legal highs using the FIA-HRMS(ddMS/HRMS) method developed, are discussed to illustrate the screening strategy used in this study. The results showed that many of the analyzed samples were adulterated, and in some cases the sample composition did not match that of the supposed marketed substance.

Simplex optimization of the variables influencing the determination of pefloxacin by time-resolved chemiluminescence

NASA Astrophysics Data System (ADS)

Murillo Pulgarín, José A.; Alañón Molina, Aurelia; Jiménez García, Elisa

2018-03-01

A new chemiluminescence (CL) detection system combined with flow injection analysis (FIA) for the determination of Pefloxacin is proposed. The determination is based on an energy transfer from Pefloxacin to terbium (III). The metal ion enhances the weak CL signal produced by the KMnO4/H2SO3/Pefloxacin system. A modified simplex method was used to optimize chemical and instrumental variables. The influence of the interaction of the permanganate, Tb (III), sodium sulphite and sulphuric acid concentrations, flow rate and injected sample volume was thoroughly investigated by using a modified simplex optimization procedure. The results revealed a strong direct relationship between flow rate and CL intensity throughout the studied range that was confirmed by a gamma test. The response factor for the CL emission intensity was used to assess performance in order to identify the optimum conditions for maximization of the response. Under such conditions, the CL response was proportional to the Pefloxacin concentration over a wide range. The detection limit as calculated according to Clayton's criterion 13.7 μg L- 1. The analyte was successfully determined in milk samples with an average recovery of 100.6 ± 9.8%.

Analytical methodologies for aluminium speciation in environmental and biological samples--a review.

PubMed

Bi, S P; Yang, X D; Zhang, F P; Wang, X L; Zou, G W

2001-08-01

It is recognized that aluminium (Al) is a potential environmental hazard. Acidic deposition has been linked to increased Al concentrations in natural waters. Elevated levels of Al might have serious consequences for biological communities. Of particular interest is the speciation of Al in aquatic environments, because Al toxicity depends on its forms and concentrations. In this paper, advances in analytical methodologies for Al speciation in environmental and biological samples during the past five years are reviewed. Concerns about the specific problems of Al speciation and highlights of some important methods are elucidated in sections devoted to hybrid techniques (HPLC or FPLC coupled with ET-AAS, ICP-AES, or ICP-MS), flow-injection analysis (FIA), nuclear magnetic resonance (27Al NMR), electrochemical analysis, and computer simulation. More than 130 references are cited.

Determination of organic peroxides by liquid chromatography with on-line post-column ultraviolet irradiation and peroxyoxalate chemiluminescence detection.

PubMed

Wada, Mitsuhiro; Inoue, Keiyu; Thara, Ayuko; Kishikawa, Naoya; Nakashima, Kenichiro; Kuroda, Naotaka

2003-02-14

A HPLC method was developed for the simultaneous determination of organic peroxides and hydrogen peroxide with peroxyoxalate chemiluminescence (PO-CL) detection following on-line UV irradiation. Organic peroxides [i.e., benzoyl peroxide (BP), tert.-butyl hydroperoxide (BHP), tert.-butyl perbenzoate (BPB), cumene hydroperoxide (CHP)] were UV irradiated (254 nm, 15 W) to generate hydrogen peroxide, which was determined by PO-CL detection. The conditions for UV irradiation and PO-CL detection were optimized by a flow injection analysis (FIA) system. Generation of hydrogen peroxide from peroxides with on-line UV irradiation also was confirmed by the FIA system by incorporating an enzyme column reactor immobilized with catalase. The separation of four organic peroxides and hydrogen peroxide by HPLC was accomplished isocratically on an ODS column within 30 min. The detection limits (signal-to-noise ratio=3) were 1.1 microM for hydrogen peroxide, 6.8 microM for BP, 31.3 microM for BHP, 7.5 microM for BPB and 1.3 microM for CHP. The proposed method was applied to the determination of BP in wheat flour.

A rapid, automated approach to optimisation of multiple reaction monitoring conditions for quantitative bioanalytical mass spectrometry.

PubMed

Higton, D M

2001-01-01

An improvement to the procedure for the rapid optimisation of mass spectrometry (PROMS), for the development of multiple reaction methods (MRM) for quantitative bioanalytical liquid chromatography/tandem mass spectrometry (LC/MS/MS), is presented. PROMS is an automated protocol that uses flow-injection analysis (FIA) and AppleScripts to create methods and acquire the data for optimisation. The protocol determines the optimum orifice potential, the MRM conditions for each compound, and finally creates the MRM methods needed for sample analysis. The sensitivities of the MRM methods created by PROMS approach those created manually. MRM method development using PROMS currently takes less than three minutes per compound compared to at least fifteen minutes manually. To further enhance throughput, approaches to MRM optimisation using one injection per compound, two injections per pool of five compounds and one injection per pool of five compounds have been investigated. No significant difference in the optimised instrumental parameters for MRM methods were found between the original PROMS approach and these new methods, which are up to ten times faster. The time taken for an AppleScript to determine the optimum conditions and build the MRM methods is the same with all approaches. Copyright 2001 John Wiley & Sons, Ltd.

Flow injection/atomic absorption spectrometric determination of zineb in commercial formulations of pesticide based on slurry sampling.

PubMed

Cassella, Ricardo J; Salim, Verĵnica A; Garrigues, Salvador; Santelli, Ricardo E; de la Guardia, Miguel

2002-11-01

This paper reports on a new strategy for the slurry sampling determination of dithiocarbamate pesticide zineb [[ethylenebis(dithiocarbamato)]zinc] employing a FIA system with a flame atomic absorption spectrometry detector. In the flow system, an on-line alkaline hydrolysis of the pesticide is performed, allowing the release of Zn(II) ions to the solution, which are easily detected by a flame AAS technique. Several parameters that could affect the performance of the analytical methodology were studied, such as the concentration of NH3(aq) used in the hydrolysis step, the effect of the presence of Triton X-100 on the sensitivity and precision, and the FIA parameters (carrier flow rate and mixing coil volume). Under optimized conditions, aqueous slurries containing 2.5 to 25 microg ml(-1) zineb provided good linear calibration fits. From the obtained data, a detection limit (3sigma) of 1.0 microg ml(-1) zineb was found and a repeatability of 2.7% was obtained from 12 measurements of a slurry containing 2.5 microg m(-1) zineb. On the other hand, a precision (reproducibility) of 7.8% was achieved from three determinations of a sample containing 128 mg g(-1) of the pesticide. Also, the developed system provides a sampling frequency of 72 h(-1).

Determination of fatty acid methyl esters in cosmetic castor oils by flow injection - electrospray ionization - high resolution mass spectrometry.

PubMed

Arroyo Negrete, Missael Antonio; Wrobel, Kazimierz; Acevedo Aguilar, Francisco Javier; Yanez Barrientos, Eunice; Corrales Escobosa, Alma Rosa; Wrobel, Katarzyna

2018-05-09

The goal of this work was to set up a high throughput procedure for the determination of fatty acid methyl esters (FAMEs) in cosmetic castor oils using flow injection - electrospray ionization - high resolution mass spectrometry, and to demonstrate the need of such analysis for the quality control purposes. The sample aliquot was mixed with isooctane:chloroform (1:1) and submitted to transesterification; the obtained FAMEs were appropriately diluted using water:isopropanol:acetonitrile (20:50:30) with addition of sodium formate which served as an internal standard, lock mass calibrant and promoted the formation of sodium adducts during electrospray ionization (ESI). The principle of flow injection analysis (FIA) was applied for sample introduction to an ESI - quadrupole- time of flight mass spectrometer (ESI-QTOFMS). The carrier solution was composed of water:isopropanol:acetonitrile (20:50:30). From the acquired MS data, flowgrams of the extracted [M+Na] + ions were obtained using the following m/z values for individual FAMEs: 293.2451 (C16:0); 315.2295 (C18:3); 317.2451 (C18:2); 319.2608 (C18:1); 321.2764 (C18:0); 335.2557 (C18:1,OH); 349.3077 (C20:0); 377.3390 (C22:0) and m/z 226.9515 for IS. Baseline-subtracted and filtered signals were integrated and the list of peaks intensities was exported to Excel, where calibration functions were obtained and quantification carried out. Gas chromatography with a flame ionization detector (GC-FID) was used as an alternative analytical tool. The calibration detection limits for FAMEs of unsaturated fatty acids were in the range 3.61 - 8.62 μg L -1 and for saturated compounds in the range 8.51 - 82.4 μg L -1 . The results obtained for commercial were in good agreement with GC-FID data; among nine cosmetic oils analyzed, three contained low concentrations of ricinoleic acid (C18:1, OH), indicating adulteration of castor bean oil with other vegetable oils. Application of FIA for the sample introduction to ESI-QTOFMS enabled for reliable determination of FAMEs in cosmetic oils with sampling frequency of thirty per hour as compared to two samples per hour achievable using GC-FID. The proposed procedure is especially well suited for FAMEs of unsaturated fatty acids that are primary components of castor triacylglycerides, and contribute to desirable properties of any cosmetic oil. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

FIA-automated system used to electrochemically measure nitrite and its interfering chemicals through a 1-2 DAB / Au electrode: gain of sensitivity at upper potentials

NASA Astrophysics Data System (ADS)

Almeida, F. L.; dos Santos Filho, S. G.; Fontes, M. B. A.

2013-03-01

The measurement of nitrite and its interfering-chemicals (paracetamol, ascorbic acid and uric acid) was performed employing a Flow-injection Analysis (FIA) system, which was automated using solenoid valves and air-pump. It is very important to quantify nitrite from river water, food and biologic fluids due to its antibacterial capacity in moderated concentrations, or its toxicity for human health even at low concentrations (> 20 μmol L-1 in blood fluids). Electrodes of the electrochemical planar sensor were defined by silk-screen technology. The measuring electrode was made from gold paste covered with 1-2 cis Diaminobenzene (DAB), which allowed good selectivity, linearity, repeatability, stability and optimized gain of sensitivity at 0.5 VAg/AgCl Nafion®117 (6.93 μA mol-1 L mm-2) compared to 0.3 VAg/AgCl Nafion® 117. The reference electrode was obtained from silver/palladium paste modified with chloride and covered with Nafion® 117. The auxiliary electrode was made from platinum paste. It was noteworthy that nitrite response adds to the response of the studied interfering-chemicals and it is predominant for concentrations lower than 175 μmol L-1.

Remote calorimetric detection of urea via flow injection analysis

PubMed Central

Gaddes, David E.; Demirel, Melik C.; Reeves, W. Brian; Tadigadapa, Srinivas

2017-01-01

The design and development of a calorimetric biosensing system enabling relatively high throughput sample analysis are reported. The calorimetric biosensor system consists of a thin (~20 μm) micromachined Y-cut quartz crystal resonator (QCR) as a temperature sensor placed in close proximity to a fluidic chamber packed with an immobilized enzyme. Layer by layer enzyme immobilization of urease is demonstrated and its activity as a function of the number of layers, pH, and time has been evaluated. This configuration enables a sensing system where a transducer element is physically separated from the analyte solution of interest and is thereby free from fouling effects typically associated with biochemical reactions occuring on the sensor surface. The performance of this biosensing system is demonstrated by detection of 1–200 mM urea in phosphate buffer via a flow injection analysis (FIA) technique. Miniaturized fluidic systems were used to provide continuous flow through a reaction column. Under this configuration the biosensor has an ultimate resolution of less than 1 mM urea and showed a linear response between 0–50 mM. This work demonstrates a sensing modality in which the sensor itself is not fouled or contaminated by the solution of interest and the enzyme immobilized Kapton® fluidic reaction column can be used as a disposable cartridge. Such a system enables reuse and reliability for long term sampling measurements. Based on this concept a biosensing system is envisioned which can perform rapid measurements to detect biomarkers such as glucose, creatinine, cholesterol, urea and lactate in urine and blood continuously over extended periods of time. PMID:26479269

Remote calorimetric detection of urea via flow injection analysis.

PubMed

Gaddes, David E; Demirel, Melik C; Reeves, W Brian; Tadigadapa, Srinivas

2015-12-07

The design and development of a calorimetric biosensing system enabling relatively high throughput sample analysis are reported. The calorimetric biosensor system consists of a thin (∼20 μm) micromachined Y-cut quartz crystal resonator (QCR) as a temperature sensor placed in close proximity to a fluidic chamber packed with an immobilized enzyme. Layer by layer enzyme immobilization of urease is demonstrated and its activity as a function of the number of layers, pH, and time has been evaluated. This configuration enables a sensing system where a transducer element is physically separated from the analyte solution of interest and is thereby free from fouling effects typically associated with biochemical reactions occuring on the sensor surface. The performance of this biosensing system is demonstrated by detection of 1-200 mM urea in phosphate buffer via a flow injection analysis (FIA) technique. Miniaturized fluidic systems were used to provide continuous flow through a reaction column. Under this configuration the biosensor has an ultimate resolution of less than 1 mM urea and showed a linear response between 0-50 mM. This work demonstrates a sensing modality in which the sensor itself is not fouled or contaminated by the solution of interest and the enzyme immobilized Kapton® fluidic reaction column can be used as a disposable cartridge. Such a system enables reuse and reliability for long term sampling measurements. Based on this concept a biosensing system is envisioned which can perform rapid measurements to detect biomarkers such as glucose, creatinine, cholesterol, urea and lactate in urine and blood continuously over extended periods of time.

Flow injection method for the determination of silver concentration in drinking water for spacecrafts.

PubMed

Bruzzoniti, Maria Concetta; Kobylinska, Dorota Korte; Franko, Mladen; Sarzanini, Corrado

2010-04-14

A flow injection method has been developed for determination of silver. The method is based on a reduction reaction with sodium borohydride which leads to the formation of a colloidal species which is monitored at a wavelength of 390 nm. The reaction variables flow rate, sodium borohydride concentration and pH, which affect sensitivity, were investigated and their effects were established using a two-levels, three-factor experimental design. Further optimization of manifold variables (reaction coil and injection volume) allowed us to determine silver in the range 0.050-5.0 mg L(-1) with a minimum detectable concentration of 0.050 mg L(-1). Silver is added, as biocide, to drinking water for spacecrafts. The chemical species of silver, present in this kind of sample, were characterized by a procedure based on the selective retention of Ag(+) onto a 2.2.2. cryptand based substrate followed by determination of the non-bound and bound (after elution) Ag(+) by the FIA method. The method optimized was applied to a drinking water sample provided for the launch with the Automated Transfer Vehicle (ATV) module Jule Verne to the International Space Station (March 9, 2008). Copyright 2010 Elsevier B.V. All rights reserved.

Simultaneous determination of Cr(III) and Cr(VI) in tannery wastewater using low pressure ion chromatography combined with flow injection spectrophotometry

NASA Astrophysics Data System (ADS)

Chen, Shujuan; Zhang, Xinshen; Yu, Lingyun; Wang, Li; Li, Hui

2012-03-01

Trivalent and hexavalent chromium have been successfully separated and determined using low pressure ion chromatography combined with flow injection spectrophotometric analysis (LPIC-FIA). A column packed with crosslinking starch microspheres was used for on-line separation of Cr(III) from Cr(VI) in a flow-injection system because of its absorptive effect on Cr(III). To determine the concentration of Cr(III) and Cr(VI) in samples, we used 3.0 mmol/L nitric acid to elute adsorbed Cr(III) from the column and then used ceric sulfate-sulfuric acid as oxidant to convert all Cr(III) into Cr(VI). Then, Cr(VI) directly came from the samples and Cr(VI) came from Cr(III) successively formed a amaranthine complex with diphenycarbazide and the complex shows a maximum absorption at 530 nm. Analytical parameters including the concentration of eluent and oxidant solution, oxidizing temperature, length of oxidizing reaction coil, reaction coil and injection coil, interfering effects, etc., were optimized. The limit of detection was 1.25 μg/L for Cr(VI) and 3.76 μg/L for Cr(III). The linear relationship between absorption with the concentration of Cr(VI) and Cr(III) was 0.001-1.000 mg/L and 0.030-1.000 mg/L with correlation coefficients of 0.9995 and 0.9994, respectively. The relative standard deviation of Cr(VI) and Cr(III) was 1.21% and 1.66%, respectively (n = 10). Major cations and anions did not show any interference. We validated this method through certified reference materials and through measuring the recovery in tannery wastewater.

Double injection/single detection asymmetric flow injection manifold for spectrophotometric determination of ascorbic acid and uric acid: Selection the optimal conditions by MCDM approach based on different criteria weighting methods.

PubMed

Boroumand, Samira; Chamjangali, Mansour Arab; Bagherian, Ghadamali

2017-03-05

A simple and sensitive double injection/single detector flow injection analysis (FIA) method is proposed for the simultaneous kinetic determination of ascorbic acid (AA) and uric acid (UA). This method is based upon the difference between the rates of the AA and UA reactions with Fe 3+ in the presence of 1, 10-phenanthroline (phen). The absorbance of Fe 2+ /1, 10-phenanthroline (Fe-phen) complex obtained as the product was measured spectrophotometrically at 510nm. To reach a good accuracy in the differential kinetic determination via the mathematical manipulations of the transient signals, different criteria were considered in the selection of the optimum conditions. The multi criteria decision making (MCDM) approach was applied for the selection of the optimum conditions. The importance weights of the evaluation criteria were determined using the analytic hierarchy process, entropy method, and compromised weighting (CW). The experimental conditions (alternatives) were ranked by the technique for order preference by similarity to an ideal solution. Under the selected optimum conditions, the obtained analytical signals were linear in the ranges of 0.50-5.00 and 0.50-4.00mgL -1 for AA and UA, respectively. The 3σ detection limits were 0.07mgL -1 for AA and 0.12mgL -1 for UA. The relative standard deviations for four replicate determinations of AA and UA were 2.03% and 3.30% respectively. The method was also applied for the analysis of analytes in the blood serum, Vitamine C tablets, and tap water with satisfactory results. Copyright © 2016. Published by Elsevier B.V.

Double injection/single detection asymmetric flow injection manifold for spectrophotometric determination of ascorbic acid and uric acid: Selection the optimal conditions by MCDM approach based on different criteria weighting methods

NASA Astrophysics Data System (ADS)

Boroumand, Samira; Chamjangali, Mansour Arab; Bagherian, Ghadamali

2017-03-01

A simple and sensitive double injection/single detector flow injection analysis (FIA) method is proposed for the simultaneous kinetic determination of ascorbic acid (AA) and uric acid (UA). This method is based upon the difference between the rates of the AA and UA reactions with Fe3 + in the presence of 1, 10-phenanthroline (phen). The absorbance of Fe2 +/1, 10-phenanthroline (Fe-phen) complex obtained as the product was measured spectrophotometrically at 510 nm. To reach a good accuracy in the differential kinetic determination via the mathematical manipulations of the transient signals, different criteria were considered in the selection of the optimum conditions. The multi criteria decision making (MCDM) approach was applied for the selection of the optimum conditions. The importance weights of the evaluation criteria were determined using the analytic hierarchy process, entropy method, and compromised weighting (CW). The experimental conditions (alternatives) were ranked by the technique for order preference by similarity to an ideal solution. Under the selected optimum conditions, the obtained analytical signals were linear in the ranges of 0.50-5.00 and 0.50-4.00 mg L- 1 for AA and UA, respectively. The 3σ detection limits were 0.07 mg L- 1 for AA and 0.12 mg L- 1 for UA. The relative standard deviations for four replicate determinations of AA and UA were 2.03% and 3.30% respectively. The method was also applied for the analysis of analytes in the blood serum, Vitamine C tablets, and tap water with satisfactory results.

Determination of Noncovalent Binding Using a Continuous Stirred Tank Reactor as a Flow Injection Device Coupled to Electrospray Ionization Mass Spectrometry

NASA Astrophysics Data System (ADS)

Santos, Inês C.; Waybright, Veronica B.; Fan, Hui; Ramirez, Sabra; Mesquita, Raquel B. R.; Rangel, António O. S. S.; Fryčák, Petr; Schug, Kevin A.

2015-07-01

Described is a new method based on the concept of controlled band dispersion, achieved by hyphenating flow injection analysis with ESI-MS for noncovalent binding determinations. A continuous stirred tank reactor (CSTR) was used as a FIA device for exponential dilution of an equimolar host-guest solution over time. The data obtained was treated for the noncovalent binding determination using an equimolar binding model. Dissociation constants between vancomycin and Ac-Lys(Ac)-Ala-Ala-OH peptide stereoisomers were determined using both the positive and negative ionization modes. The results obtained for Ac- L-Lys(Ac)- D-Ala- D-Ala (a model for a Gram-positive bacterial cell wall) binding were in reasonable agreement with literature values made by other mass spectrometry binding determination techniques. Also, the developed method allowed the determination of dissociation constants for vancomycin with Ac- L-Lys(Ac)- D-Ala- L-Ala, Ac- L-Lys(Ac)- L-Ala- D-Ala, and Ac- L-Lys(Ac)- L-Ala- L-Ala. Although some differences in measured binding affinities were noted using different ionization modes, the results of each determination were generally consistent. Differences are likely attributable to the influence of a pseudo-physiological ammonium acetate buffer solution on the formation of positively- and negatively-charged ionic complexes.

Optimization by infusion of multiple reaction monitoring transitions for sensitive quantification of peptides by liquid chromatography/mass spectrometry.

PubMed

Alghanem, Bandar; Nikitin, Frédéric; Stricker, Thomas; Duchoslav, Eva; Luban, Jeremy; Strambio-De-Castillia, Caterina; Muller, Markus; Lisacek, Frédérique; Varesio, Emmanuel; Hopfgartner, Gérard

2017-05-15

In peptide quantification by liquid chromatography/mass spectrometry (LC/MS), the optimization of multiple reaction monitoring (MRM) parameters is essential for sensitive detection. We have compared different approaches to build MRM assays, based either on flow injection analysis (FIA) of isotopically labelled peptides, or on the knowledge and the prediction of the best settings for MRM transitions and collision energies (CE). In this context, we introduce MRMOptimizer, an open-source software tool that processes spectra and assists the user in selecting transitions in the FIA workflow. MS/MS spectral libraries with CE voltages from 10 to 70 V are automatically acquired in FIA mode for isotopically labelled peptides. Then MRMOptimizer determines the optimal MRM settings for each peptide. To assess the quantitative performance of our approach, 155 peptides, representing 84 proteins, were analysed by LC/MRM-MS and the peak areas were compared between: (A) the MRMOptimizer-based workflow, (B1) the SRMAtlas transitions set used 'as-is'; (B2) the same SRMAtlas set with CE parameters optimized by Skyline. 51% of the three most intense transitions per peptide were shown to be common to both A and B1/B2 methods, and displayed similar sensitivity and peak area distributions. The peak areas obtained with MRMOptimizer for transitions sharing either the precursor ion charge state or the fragment ions with the SRMAtlas set at unique transitions were increased 1.8- to 2.3-fold. The gain in sensitivity using MRMOptimizer for transitions with different precursor ion charge state and fragment ions (8% of the total), reaches a ~ 11-fold increase. Isotopically labelled peptides can be used to optimize MRM transitions more efficiently in FIA than by searching databases. The MRMOptimizer software is MS independent and enables the post-acquisition selection of MRM parameters. Coefficients of variation for optimal CE values are lower than those obtained with the SRMAtlas approach (B2) and one additional peptide was detected. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

Determination of water traces in various organic solvents using Karl Fischer method under FIA conditions.

PubMed

Dantan, N; Frenzel, W; Küppers, S

2000-05-31

Flow injection methods utilising the Karl Fischer (KF) reaction with spectrophotometric and potentiometric detection are described for the determination of the trace water content in various organic solvents. Optimisation of the methods resulted in an accessible (linear) working range of 0.01-0.2% water for many solvents studied with a typical precision of 1-2% R.S.D. Only 50 mul of organic solvent was injected and the sampling frequency was about 120 samples per h. Since the slopes of the calibration curves were different for different solvents appropriate calibration was required. Problems associated with spectrophotometric detection and caused by refractive index changes were pointed out and a nested-loop configuration was proposed to overcome this kind of interference. The potentiometric method with a novel flow-through detector cell was shown to surpass the performance of spectrophotometric detection in any respect. The characteristics of the procedures developed made them well applicable for on-line monitoring of technical solvent distillations in an industrial plant.

Chemometric approach to evaluate element distribution in muscle, liver and fish bone of roach (Rutilus rutilus), silver bream (Blicca bjoerkna) and crucian carp (Carassius carassius) from Swarzędzkie Lake (Poland) using ICP-MS and FIAS-CVAAS techniques.

PubMed

Chudzińska, Maria; Komorowicz, Izabela; Hanć, Anetta; Gołdyn, Ryszard; Barałkiewicz, Danuta

2016-11-01

The content of elements in fish tissues and organs from Swarzędzkie Lake was investigated in order to evaluate the possible risk associated with their consumption by animals as well as humans. Samples of muscle, liver and fish bone of three fish species; roach (Rutilus rutilus), silver bream (Blicca bjoerkna) and crucian carp (Carassius carassius) were collected from seine catches undertaken as part of the biomanipulation of Swarzędzkie Lake. Element concentration (Al, As, Cd, Co, Cr, Cu, Hg, Ni, Pb, Zn) was determined by inductively coupled plasma mass spectrometry (ICP-MS), with the exception of Hg where the flow injection analysis system cold vapour atomic absorption spectrometry (FIAS-CVAAS) was applied. The study indicated a large variation in the occurrence of the investigated elements in different parts of the fish body. The highest content of Al and Zn was stated in all fish organs for each fish species. The majority of the applied statistical and chemometric methods (e.g., PCA, CA) refer to roach since we had a large number of data for this species. The obtained results were assessed in terms of their accuracy and precision using certified reference material of Fish Muscle ERM BB422.

Confocal laser-induced fluorescence detector for narrow capillary system with yoctomole limit of detection.

PubMed

Weaver, Mitchell T; Lynch, Kyle B; Zhu, Zaifang; Chen, Huang; Lu, Joann J; Pu, Qiaosheng; Liu, Shaorong

2017-04-01

Laser-induced fluorescence (LIF) detectors for low-micrometer and sub-micrometer capillary on-column detection are not commercially available. In this paper, we describe in details how to construct a confocal LIF detector to address this issue. We characterize the detector by determining its limit of detection (LOD), linear dynamic range (LDR) and background signal drift; a very low LOD (~70 fluorescein molecules or 12 yoctomole fluorescein), a wide LDR (greater than 3 orders of magnitude) and a small background signal drift (~1.2-fold of the root mean square noise) are obtained. For detecting analytes inside a low-micrometer and sub-micrometer capillary, proper alignment is essential. We present a simple protocol to align the capillary with the optical system and use the position-lock capability of a translation stage to fix the capillary in position during the experiment. To demonstrate the feasibility of using this detector for narrow capillary systems, we build a 2-μm-i.d. capillary flow injection analysis (FIA) system using the newly developed LIF prototype as a detector and obtain an FIA LOD of 14 zeptomole fluorescein. We also separate a DNA ladder sample by bare narrow capillary - hydrodynamic chromatography and use the LIF prototype to monitor the resolved DNA fragments. We obtain not only well-resolved peaks but also the quantitative information of all DNA fragments. Copyright © 2016 Elsevier B.V. All rights reserved.

A novel stopped flow injection-amperometric procedure for the determination of chlorate.

PubMed

Tue-Ngeun, Orawan; Jakmunee, Jaroon; Grudpan, Kate

2005-12-15

A novel stopped flow injection-amperometric (sFI-Amp) procedure for determination of chlorate has been developed. The reaction of chlorate with excess potassium iodide and hydrochloric acid, forming iodine/triiodide that is further electrochemically reduced at a glassy carbon electrode at +200mV versus Ag/AgCl electrode is employed. In order to increase sensitivity without using of too high acid concentration, promoting of the reaction by increasing reaction time and temperature can be carried out. This can be done without increase of dispersion of the product zone by stopping the flow while the injected zone is being in a mixing coil which is immersed in a water bath of 55+/-0.5 degrees C. In a closed system of FIA, a side reaction of oxygen with iodide is also minimized. Under a set of conditions, linear calibration graphs were in the ranges of 1.2x10(-6)-6.0x10(-5)moll(-1)and 6.0x10(-5)-6.0x10(-4)moll(-1). A sample throughput of 25h(-1) was accomplished. Relative standard deviation was 2% (n=21, 1.2x10(-4)moll(-1) chlorate). The proposed sFI-Amp procedure was successfully applied to the determination of chlorate in soil samples from longan plantation area.

Fast batch injection analysis of H(2)O(2) using an array of Pt-modified gold microelectrodes obtained from split electronic chips.

PubMed

Pacheco, Bruno D; Valério, Jaqueline; Angnes, Lúcio; Pedrotti, Jairo J

2011-06-24

A fast and robust analytical method for amperometric determination of hydrogen peroxide (H(2)O(2)) based on batch injection analysis (BIA) on an array of gold microelectrodes modified with platinum is proposed. The gold microelectrode array (n=14) was obtained from electronic chips developed for surface mounted device technology (SMD), whose size offers advantages to adapt them in batch cells. The effect of the dispensing rate, volume injected, distance between the platinum microelectrodes and the pipette tip, as well as the volume of solution in the cell on the analytical response were evaluated. The method allows the H(2)O(2) amperometric determination in the concentration range from 0.8 μmolL(-1) to 100 μmolL(-1). The analytical frequency can attain 300 determinations per hour and the detection limit was estimated in 0.34 μmolL(-1) (3σ). The anodic current peaks obtained after a series of 23 successive injections of 50 μL of 25 μmolL(-1) H(2)O(2) showed an RSD<0.9%. To ensure the good selectivity to detect H(2)O(2), its determination was performed in a differential mode, with selective destruction of the H(2)O(2) with catalase in 10 mmolL(-1) phosphate buffer solution. Practical application of the analytical procedure involved H(2)O(2) determination in rainwater of São Paulo City. A comparison of the results obtained by the proposed amperometric method with another one which combines flow injection analysis (FIA) with spectrophotometric detection showed good agreement. Copyright © 2011 Elsevier B.V. All rights reserved.

Design of a microfluidic sensor for high-sensitivity Copper (II) sensing applications

NASA Astrophysics Data System (ADS)

Gibson, Ceri; Byrne, Patrick; Gray, David; MacCraith, Brian D.; Paull, Brett; Tyrrell, Eadaoin

2003-03-01

An all-plastic micro-sensor system for remote measurement of copper (II) ions in the aqueous environment has been developed. The sensing structure was designed for ease of milling and fabricated in poly (methyl methacrylate) (PMMA) using a hot-embossing technique. Issues of sealing the structure were studied extensively and an efficient protocol has been established. The detection system comprises a compact photo-multiplier tube and integrated photon counting system. This method has advantages of low sample volume, (creating a minimal volume of waste), low exposure to contaminants due to the closed system, no moving parts and employs a robust polymer material which is resistant to the environment of intended use. The sensor operates on the principle of flow injection analysis and has been tested using a chemiluminescence (FIA-CL) reaction arising from the complexation of copper with 1,10-phenanthroline and subsequent oxidation by hydrogen peroxide.

Simple flow injection analysis system for simultaneous determination of phenolic antioxidants with multiple pulse amperometric detection at a boron-doped diamond electrode.

PubMed

Medeiros, Roberta Antigo; Lourenção, Bruna Cláudia; Rocha-Filho, Romeu Cardozo; Fatibello-Filho, Orlando

2010-10-15

A method for simultaneous determination of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) in food was developed that uses multiple pulse amperometry (MPA) with flow injection analysis (FIA). Determination of these phenolic antioxidants was carried out with a cathodically pretreated boron-doped diamond electrode and an aqueous ethanolic (30% ethanol, v/v) 10 mmol L⁻¹ KNO₃ solution (pH(cond) = 1.5) as supporting electrolyte. A dual-potential waveform, at E(det1) = 850 mV/200 ms and E(det2) = 1150 mV/200 ms versus Ag/AgCl (3.0 mol L⁻¹ KCl), was employed. The use of E(det1) or E(det2) caused the oxidation of BHA or of BHA and BHT, respectively; hence, concentration subtraction could be used to determine both species. The respective analytical curves presented good linearity in the investigated concentration range (0.050-3.0 μmol L⁻¹ for BHA and 0.70-70 μmol L⁻¹ for BHT), and the detection limits were 0.030 μmol L⁻¹ for BHA and 0.40 μmol L⁻¹ for BHT. The proposed method, which is simple, quick, and presents good precision and accuracy, was successfully applied in the simultaneous determination of BHA and BHT in commercial mayonnaise samples, with results similar to those obtained by HPLC, at a 95% confidence level.

A cohabitation challenge to compare the efficacies of vaccines for bacterial kidney disease (BKD) in chinook salmon Oncorhynchus tshawytscha

USGS Publications Warehouse

Alcorn, S.; Murray, A.L.; Pascho, R.J.; Varney, J.

2005-01-01

The relative efficacies of 1 commercial and 5 experimental vaccines for bacterial kidney disease (BKD) were compared through a cohabitation waterborne challenge. Groups of juvenile chinook salmon Oncorhynchus tshawytscha were vaccinated with one of the following: (1) killed Renibacterium salmoninarum ATCC 33209 (Rs 33209) cells; (2) killed Rs 33209 cells which had been heated to 37??C for 48 h, a process that destroys the p57 protein; (3) killed R. salmoninarum MT239 (Rs MT239) cells; (4) heated Rs MT239 cells; (5) a recombinant version of the p57 protein (r-p57) emulsified in Freund's incomplete adjuvant (FIA); (6) the commercial BKD vaccine Renogen; (7) phosphate-buffered saline (PBS) emulsified with an equal volume of FIA; or (8) PBS alone. Following injection, each fish was marked with a subcutaneous fluorescent latex tag denoting its treatment group and the vaccinated fish were combined into sham and disease challenge tanks. Two weeks after these fish were vaccinated, separate groups of fish were injected with either PBS or live R. salmoninarum GL64 and were placed inside coated-wire mesh cylinders (liveboxes) in the sham and disease challenge tanks, respectively. Mortalities in both tanks were recorded for 285 d. Any mortalities among the livebox fish were replaced with an appropriate cohort (infected with R. salmoninarum or healthy) fish. None of the bacterins evaluated in this study induced protective immunity against the R. salmoninarum shed from the infected livebox fish. The percentage survival within the test groups in the R. salmoninarum challenge tank ranged from 59% (heated Rs MT239 bacterin) to 81 % (PBS emulsified with FIA). There were no differences in the percentage survival among the PBS-, PBS/FIA-, r-p57-and Renogen-injected groups. There also were no differences in survival among the bacterin groups, regardless of whether the bacterial cells had been heated or left untreated prior to injection. ?? Inter-Research 2005.

Coulometric determination of NAD+ and NADH in normal and cancer cells using LDH, RVC and a polymer mediator.

PubMed

Torabi, F; Ramanathan, K; Larsson, P O; Gorton, L; Svanberg, K; Okamoto, Y; Danielsson, B; Khayyami, M

1999-11-15

An electrochemical method for the measurement of NAD(+) and NADH in normal and cancer tissues using flow injection analysis (FIA) is reported. Reticulated vitreous carbon (RVC) electrodes with entrapped l-lactate dehydrogenase (LDH) and a new redox polymer containing covalently bound toluidine blue O (TBO) were employed for this purpose. Both NAD(+) and NADH were estimated coulometrically based on their reaction with LDH. The latter was immobilized on controlled pore glass (CPG) by cross-linking with glutaraldehyde and packed within the RVC. The concentrations of NAD(+) and NADH in the tissues, estimated using different electron mediators such as ferricyanide (FCN), meldola blue (MB) and TBO have also been compared. The effects of flow rate, pH, applied potential (versus Ag/AgCl reference) and adsorption of the mediators have also been investigated. Based on the measurements of NAD(+) and NADH in normal and cancer tissues it has been concluded that the NADH concentration is lower, while the NAD(+) concentration is higher in cancer tissues. Amongst the electron mediators TBO was found to be a more stable mediator for such measurements.

Doppler Endoscopic Probe as a Guide to Risk Stratification and Definitive Hemostasis of Peptic Ulcer Bleeding

PubMed Central

Jensen, Dennis M.; Ohning, Gordon V.; Kovacs, Thomas OG; Ghassemi, Kevin A.; Jutabha, Rome; Dulai, Gareth S.; Machicado, Gustavo A.

2015-01-01

Background and Aims For more than 4 decades endoscopists have relied on ulcer stigmata for risk stratification and as a guide to hemostasis. None used arterial blood flow underneath stigmata to predict outcomes. For patients with severe peptic ulcer bleeding (PUBs), we used Doppler endoscopic probe (DEP) for: 1. detection of blood flow underlying stigmata of recent hemorrhage (SRH), 2. quantitating rates of residual arterial blood flow under SRH after visually directed standard endoscopic treatment, and 3. comparing risks of rebleeding and actual 30 day rebleed rates for spurting arterial bleeding (Forrest – FIA) and oozing bleeding (FIB). Methods Prospective cohort study of 163 consecutive patients with severe PUBs and different SRH. Results All blood flow detected by DEP was arterial. Detection rates were 87.4% in major SRH - spurting arterial bleeding (FIA), non bleeding visible vessel (FIIA), clot (FIIB) - and significantly lower at 42.3% (p<0.0001) for intermediate group of oozing bleeding (FIB) or flat spot (FIIC). For spurting bleeding (FIA) vs. oozing (FIB), baseline DEP arterial flow was 100% vs. 46.7%; residual blood flow detected after endoscopic hemostasis was 35.7% vs. 0%; and 30 day rebleed rates were 28.6% vs. 0% (all p<0.05). Conclusions 1. For major SRH vs. oozing or spot, the arterial blood flow detection rates by DEP was significantly higher, indicating a higher rebleed risk. 2. Before and after endoscopic treatment, spurting FIA PUB’s had significantly higher rates of blood flow detection than oozing FIB PUB’s and a significantly higher 30 rebleed rate. 3. DEP is recommended as a new endoscopic guide with SRH to improve risk stratification and potentially definitive hemostasis for PUBs. PMID:26318834

Sampling protocol, estimation, and analysis procedures for the down woody materials indicator of the FIA program

Treesearch

Christopher Woodall

2005-01-01

The Forest Inventory and Analysis (FIA) program of the USDA Forest Service conducts a national inventory of forests of the United States. A subset of FIA permanent inventory plots are sampled every year for numerous indicators of forest health ranging from soils to understory vegetation. Down woody material (DWM) is an FIA indicator that provides estimates of forest...

Elemental ratios for characterization of quantum-dots populations in complex mixtures by asymmetrical flow field-flow fractionation on-line coupled to fluorescence and inductively coupled plasma mass spectrometry.

PubMed

Menendez-Miranda, Mario; Fernandez-Arguelles, Maria T; Costa-Fernandez, Jose M; Encinar, Jorge Ruiz; Sanz-Medel, Alfredo

2014-08-11

Separation and identification of nanoparticles of different composition, with similar particle diameter, coexisting in heterogeneous suspensions of polymer-coated CdSe/ZnS quantum dots (QDs) have been thoroughly assessed by asymmetric flow field-flow fractionation (AF4) coupled on-line to fluorescence and inductively coupled plasma mass spectrometry (ICPMS) detectors. Chemical characterization of any previously on-line separated nanosized species was achieved by the measurement of the elemental molar ratios of every element involved in the synthesis of the QDs, using inorganic standards and external calibration by flow injection analysis (FIA). Such elemental molar ratios, strongly limited so far to pure single nanoparticles suspensions, have been achieved with adequate accuracy by coupling for the first time an ICP-QQQ instrument to an AF4 system. This hyphenation turned out to be instrumental to assess the chemical composition of the different populations of nanoparticles coexisting in the relatively complex mixtures, due to its capabilities to detect the hardly detectable elements involved in the synthesis. Interestingly such information, complementary to that obtained by fluorescence, was very valuable to detect and identify unexpected nanosized species, present at significant level, produced during QDs synthesis and hardly detectable by standard approaches. Copyright © 2014. Published by Elsevier B.V.

Simple and ultra-fast recognition and quantitation of compounded monoclonal antibodies: Application to flow injection analysis combined to UV spectroscopy and matching method.

PubMed

Jaccoulet, E; Schweitzer-Chaput, A; Toussaint, B; Prognon, P; Caudron, E

2018-09-01

Compounding of monoclonal antibody (mAbs) constantly increases in hospital. Quality control (QC) of the compounded mAbs based on quantification and identification is required to prevent potential errors and fast method is needed to manage outpatient chemotherapy administration. A simple and ultra-fast (less than 30 s) method using flow injection analysis associated to least square matching method issued from the analyzer software was performed and evaluated for the routine hospital QC of three compounded mAbs: bevacizumab, infliximab and rituximab. The method was evaluated through qualitative and quantitative parameters. Preliminary analysis of the UV absorption and second derivative spectra of the mAbs allowed us to adapt analytical conditions according to the therapeutic range of the mAbs. In terms of quantitative QC, linearity, accuracy and precision were assessed as specified in ICH guidelines. Very satisfactory recovery was achieved and the RSD (%) of the intermediate precision were less than 1.1%. Qualitative analytical parameters were also evaluated in terms of specificity, sensitivity and global precision through a matrix of confusion. Results showed to be concentration and mAbs dependant and excellent (100%) specificity and sensitivity were reached within specific concentration range. Finally, routine application on "real life" samples (n = 209) from different batch of the three mAbs complied with the specifications of the quality control i.e. excellent identification (100%) and ± 15% of targeting concentration belonging to the calibration range. The successful use of the combination of second derivative spectroscopy and partial least square matching method demonstrated the interest of FIA for the ultra-fast QC of mAbs after compounding using matching method. Copyright © 2018 Elsevier B.V. All rights reserved.

Determination of hexavalent chromium in industrial hygiene samples using ultrasonic extraction and flow injection analysis.

PubMed

Wang, J; Ashley, K; Kennedy, E R; Neumeister, C

1997-11-01

A simple, fast, and sensitive method was developed for the determination of hexavalent chromium (CrVI) in workplace samples. Ultrasonic extraction in alkaline solutions with 0.05 M (NH4)2SO4-0.05 M NH3 provided good extraction efficiency of CrVI from the sample and allowed the retention of CrVI on an ion-exchange resin (95%). The CrVI in the sample solution was then separated as an anion from trivalent chromium [CrIII] and other cations by elution from the anion-exchange resin with 0.5 M (NH4)2SO4 in 0.1 M NH3 (pH 8) buffer solution. The eluate was then acidified with hydrochloric acid and complexed with 1,5-diphenylcarbazide reagent prior to flow injection analysis. By analyzing samples with and without oxidation of CrIII to CrVI using CeIV, the method can measure CrVI and total Cr. For optimizing the separation and determination procedure, preliminary trials conducted with two certified reference materials (CRMs 013-050 and NIST 1633a) and three spiked samples (ammonia buffer solution, cellulose ester filters and acid washed sand) indicated that the recovery of CrVI was quantitative (> 90%) with this method. The limit of detection for FIA-UV/VIS determination of the Cr-diphenylcarbazone complex was in the sub-nanogram range (0.11 ng). The technique was also applied successfully to a workplace coal fly ash sample that was collected from a power plant and paint chips that were collected from a heating gas pipe and a university building. The principal advantages of this method are its simplicity, sensitivity, speed and potential portability for field analysis.

Recent progress on mid-IR sensing with optical fibers

NASA Astrophysics Data System (ADS)

Kellner, Robert A.; Gobel, R.; Goetz, R.; Lendl, B.; Edl-Mizaikoff, B.; Tacke, Maurus; Katzir, Abraham

1995-09-01

Chemical sensors are analytical systems for the evaluation of compound- or ion-specific or - selective signals produced by specific or selective chemical reactions taking place at the interface between the chemically modified sensor surface and the substrate. The well known electrochemical sensing schemes have greatly contributed that sensors are considered now as the 'third supporting pillar of analytical chemistry' besides chromatography and spectroscopy. The aim of this paper is to describe the novel capabilities of chemical modified IR-transparent fibers as chemical IR-sensors for the on-line analysis of chlorinated hydrocarbons and organic compounds in aqueous solutions and gaseous mixtures, glucose, and sucrose in aqueous solution as developed in our laboratory. Moreover, the relative merits of this new method wil be depicted in comparison to other sensing techniques. Optical fiber sensors are novel analysis systems, based on molecular spectroscopy in the UV/VIS/IR-range. They benefit from the tremendous development in the field of optical fibers, an offspring of the telecommunication industry and the electronic revolution during the last few years. With the development of new materials besides the well known quartz fibers for the UV/VIS/NIR-range the optical window for fiber optic sensors was enlarged from 0,2 to 20 micrometers recently. The fiber length was increased recently to up to 2 meters for silver halides and approximately 10 meters for chalcogenides. New applications for environmental, food, and clinical sensing as well as process analysis are the driving force for modern research in IR-optical fiber sensors using mainly sapphire (Al2O3), chalcogenide (As-Se-Te) and silver halide (AgBr/AgCl) fibers and flow injection analysis (FIA) systems. Few representative examples for each of the various optical sensor types will be presented. Particular attention will be given to the use of silver halide fibers for the simultaneous determination of traces of chlorinated hydrocarbons in water and to FIA-systems for the process analysis of beverages.

Forests of Ohio, 2013

Treesearch

Richard H. Widmann

2014-01-01

This publication provides an overview of the forest resources in Ohio based upon inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 2001, FIA has implemented an annual inventory in Ohio....

Forests of Maryland, 2013

Treesearch

T.W. Lister; S.A. Pugh

2014-01-01

This publication provides an overview of forest resources in Maryland based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 2004, FIA has employed an annual inventory measuring data...

Forests of Maine, 2015

Treesearch

Emily S. Huff; William H. McWilliams

2016-01-01

This publication provides an overview of the forest resources in Maine based upon inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 1999, FIA has implemented an annual inventory...

Forests of Pennsylvania, 2013

Treesearch

George L. McCaskill

2014-01-01

This publication provides an overview of the forest resources in Pennsylvania based upon inventories conducted by the USDA Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 1999, FIA has implemented an annual inventory...

Forests of Maine, 2013

Treesearch

George L. McCaskill

2014-01-01

This publication provides an overview of the forest resources in Maine based upon inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 1999, FIA has implemented an annual inventory...

Opportunities to improve monitoring of temporal trends with FIA panel data

Treesearch

Raymond Czaplewski; Michael Thompson

2009-01-01

The Forest Inventory and Analysis (FIA) Program of the Forest Service, Department of Agriculture, is an annual monitoring system for the entire United States. Each year, an independent "panel" of FIA field plots is measured. To improve accuracy, FIA uses the "Moving Average" or "Temporally Indifferent" method to combine estimates from...

Forests of Maine, 2014

Treesearch

George L. McCaskill

2015-01-01

This publication provides an overview of the forest resources in Maine based upon inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 1999, FIA has implemented an annual inventory...

The effects of removing condition boundaries on FIA estimates

Treesearch

David Gartner; Gregory Reams

2002-01-01

When Forest Inverltory and Analysis (FIA) changed to the national standards for the inventory system, plots with lnultiplc condition codes were introduced to the Southern Station's FIA unit. FIA maps up to five different conditions on completely or partially forested 1124-acre subplots. This change has madc producing inventory estimates more complex because the...

Forests of Delaware, 2013

Treesearch

T.W. Lister; S.A. Pugh

2014-01-01

This publication provides an overview of forest resources in Delaware based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 2004, FIA has employed an annual inventory measuring data...

Impact of Definitions of FIA Variables and Compilation Procedures on Inventory Compilation Results in Georgia

Treesearch

Brock Stewart; Chris J. Cieszewski; Michal Zasada

2005-01-01

This paper presents a sensitivity analysis of the impact of various definitions and inclusions of different variables in the Forest Inventory and Analysis (FIA) inventory on data compilation results. FIA manuals have been changing recently to make the inventory consistent between all the States. Our analysis demonstrates the importance (or insignificance) of different...

Polyphenols Determination in Olive Oil Samples Based on a Thick Film Voltammetric Sensor and a Tyrosinase Biosensor

NASA Astrophysics Data System (ADS)

Capannesi, Cecilia; Palchetti, Ilaria; Mascini, Marco

2000-12-01

The aim of the present work was to compare different techniques to evaluate the variation with the storage time and storage conditions in the phenolic content of an extra-virgin olive oil. A disposable screen-printed sensor (SPE) was coupled with differential pulse voltammetry (DPV) to determine the phenolic fractions after extraction with glycine buffer; DPV parameters were chosen in order to study oxidation peak of oleuropein, that was used as reference compound. Moreover a tyrosinase based biosensor operating in organic solvent (hexane) was assembled, using an amperometric oxygen probe as transducer. Calibration curves were realised in flow injection analysis (F.I.A.) using phenol as substrate. Both of these methods are easy to operate, require no extraction (biosensor) or a rapid extraction procedure (SPE), and the analysis time is short (min.). The results obtained with these two innovative procedures were compared with classical spectrophotometric assay using the Folin-Ciocalteau reagent. Other extra-virgin olive oil quality parameters were investigated with classical methods in order to better define the alteration process and results are reported.

Measurement of free carnitine and acylcarnitines in plasma by HILIC-ESI-MS/MS without derivatization.

PubMed

Peng, Minzhi; Liu, Li; Jiang, Minyan; Liang, Cuili; Zhao, Xiaoyuan; Cai, Yanna; Sheng, Huiying; Ou, Zhiying; Luo, Hong

2013-08-01

Measurement of carnitine and acylcarnitines in plasma is important in diagnosis of fatty acid β-oxidation disorders and organic acidemia. The usual method uses flow injection tandem mass spectrometry (FIA-MS/MS), which has limitations. A rapid and more accurate method was developed to be used for high-risk screening and diagnosis. Carnitine and acylcarnitines were separated by hydrophilic interaction liquid chromatography (HILIC) without derivatization and detected with a QTRAP MS/MS System. Total analysis time was 9.0min. The imprecision of within- and between-run were less than 6% and 17%, respectively. Recoveries were in the range of 85-110% at three concentrations. Some acylcarnitine isomers could be separated, such as dicarboxylic and hydroxyl acylcarnitines. The method could also separate interferent to avoid false positive results. 216 normal samples and 116 patient samples were detected with the validated method, and 49 patients were identified with fatty acid oxidation disorders or organic acidemias. Copyright © 2013 Elsevier B.V. All rights reserved.

Cartographic standards to improve maps produced by the Forest Inventory and Analysis program

Treesearch

Charles H. (Hobie) Perry; Mark D. Nelson

2009-01-01

The Forest Service, U.S. Department of Agriculture's Forest Inventory and Analysis (FIA) program is incorporating an increasing number of cartographic products in reports, publications, and presentations. To create greater quality and consistency within the national FIA program, a Geospatial Standards team developed cartographic design standards for FIA map...

Forests of New York, 2013

Treesearch

Richard H. Widmann

2014-01-01

This publication provides an overview of the forest resources in New York based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at http://fia.fs.fed.us. Since 2003, FIA has implemented an annual inventory in New...

Forests of West Virginia, 2013

Treesearch

Richard H. Widmann

2014-01-01

This publication provides an overview of the forest resources in West Virginia based upon inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the FIA program is available online at http://fia.fs.fed.us. Since 2004, FIA has implemented an annual inventory in West Virginia. For...

The Effects of Removing Condition Boundaries on FIA Estimates

Treesearch

David Gartner; Gregory Reams

2005-01-01

When Forest Inventory and Analysis (FIA) changed to the national standards for the inventory system, plots with multiple condition codes were introduced to the Southern Station's FIA unit. FIA maps up to five different conditions on completely or partially forested 1/24-acre subplots. This change has made producing inventory estimates more complex because the data...

Southern forest inventory and analysis volume equation user’s guide

Treesearch

Christopher M. Oswalt; Roger C. Conner

2011-01-01

Reliable volume estimation procedures are fundamental to the mission of the Forest Inventory and Analysis (FIA) program. Moreover, public access to FIA program procedures is imperative. Here we present the volume estimation procedures used by the southern FIA program of the U.S. Department of Agriculture Forest Service Southern Research Station. The guide presented...

On FIA Variables For Ecological Use

Treesearch

David C. Chojnacky

2001-01-01

The Forest Inventory and Analysis (FIA) program collects or calculates over 300 variables for its national network of permanent forest plots. However, considerable ecological analysis can be done with only a few key variables. Two examples--Mexican spotted owl habitat in New Mexico and down deadwood in Maine--are used to illustrate the potential of FIA data for...

Moving from status to trends: Forest Inventory and Analysis (FIA) symposium 2012

Treesearch

Randall S. Morin; Greg C. Liknes

2012-01-01

These proceedings report invited presentations and contributions to the 2012 biennial Forest Inventory and Analysis (FIA) Symposium, which was hosted by the Research and Development branch of the U.S. Forest Service. As the only comprehensive and continuous census of the forests in the United States, FIA provides strategic information needed to evaluate sustainability...

Access and Use of FIA Data Through FIA Spatial Data Services

Treesearch

Elizabeth LaPoint

2005-01-01

Forest Inventory and Analysis (FIA) Spatial Data Services (SDS) was established in May 2002 to facilitate outside access to FIA data and allow use of georeferenced plot data while protecting the confidentiality of plot locations. Modification of the Food Security Act of 1985 legislated the protection of information on plot location and ownership. Penalties were put in...

Comparing Minnesota land cover/use area estimates using NRI and FIA data

Treesearch

Veronica C. Lessard; Mark H. Hansen; Mark D. Nelson

2002-01-01

Areas for land cover/use categories on non-Federal land in Minnesota were estimated from Forest Inventory and Analysis (FIA) data and National Resources Inventory (NRI) data. Six common land cover/use categories were defined, and the NRI and FIA land cover/use categories were assigned to them. Area estimates for these categories were calculated from the FIA and NRI...

Pilot Inventory of FIA plots traditionally called `nonforest'

Treesearch

Rachel Riemann

2003-01-01

Forest-inventory data were collected on plots defined as ?nonforest? by the USDA Forest Service?s Forest Inventory and Analysis (FIA) unit. Nonforest plots may have trees on them, but they do not fit FIA?s definition of forest because the area covered by trees is too small, too sparsely populated by trees, too narrow (e.g., trees between fields or in the middle of a...

Verification of the Jenkins and FIA sapling biomass equations for hardwood species in Maine

Treesearch

Andrew S. Nelson; Aaron R. Weiskittel; Robert G. Wagner; Michael R. Saunders

2012-01-01

In 2009, the Forest Inventory and Analysis Program (FIA) updated its biomass estimation protocols by switching to the component ratio method to estimate biomass of medium and large trees. Additionally, FIA switched from using regional equations to the current FIA aboveground sapling biomass equations that predict woody sapling (2.5 to 12.4 cm d.b.h.) biomass using the...

Forest Inventory and Analysis (FIA) Symposium 2008; October 21-23, 2008; Park City, UT

Treesearch

Will McWilliams; Gretchen Moisen; Ray Czaplewski

2009-01-01

These proceedings report invited presentations and contributions to the 2008 Biennial Forest Inventory and Analysis (FIA) Symposia, which was hosted by the Research and Development branch of the U.S. Forest Service. As the only comprehensive and continuous census of the forests in the USA, FIA provides strategic information needed to evaluate sustainability of current...

Pushing boundaries: new directions in inventory techniques and applications: Forest Inventory and Analysis (FIA) symposium 2015

Treesearch

Sharon M. Stanton; Glenn A. Christensen

2016-01-01

These proceedings report invited presentations and contributions to the 2015 Forest Inventory and Analysis (FIA) Symposium, which was hosted by the Research and Development branch of the U.S. Forest Service. As the only comprehensive and continuous census of the forests in the United States, FIA provides strategic information needed to evaluate sustainability of...

Maintaining the confidentiality of plot locations by exploiting the low sensitivity of forest structure models to different spectral extraction kernels

Treesearch

Sean P. Healey; Elizabeth Lapoint; Gretchen G. Moisen; Scott L. Powell

2011-01-01

The United States Forest Service Forest Inventory and Analysis (FIA) unit maintains a large national network of inventory plots.While the consistency and extent of this network make FIA data attractive for ecological modelling, the FIA is charged by statute not to publicly reveal inventory plot locations. However, use of FIA plot data by the remote sensing community...

Forests of Maine, 2016

Treesearch

Brett J. Butler

2017-01-01

This publication provides an overview of the forest resources in Maine based upon inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Information about the national and regional FIA program is available online at www.fia.fs.fed.us....

Soil Carbon Variability and Change Detection in the Forest Inventory Analysis Database of the United States

NASA Astrophysics Data System (ADS)

Wu, A. M.; Nater, E. A.; Dalzell, B. J.; Perry, C. H.

2014-12-01

The USDA Forest Service's Forest Inventory Analysis (FIA) program is a national effort assessing current forest resources to ensure sustainable management practices, to assist planning activities, and to report critical status and trends. For example, estimates of carbon stocks and stock change in FIA are reported as the official United States submission to the United Nations Framework Convention on Climate Change. While the main effort in FIA has been focused on aboveground biomass, soil is a critical component of this system. FIA sampled forest soils in the early 2000s and has remeasurement now underway. However, soil sampling is repeated on a 10-year interval (or longer), and it is uncertain what magnitude of changes in soil organic carbon (SOC) may be detectable with the current sampling protocol. We aim to identify the sensitivity and variability of SOC in the FIA database, and to determine the amount of SOC change that can be detected with the current sampling scheme. For this analysis, we attempt to answer the following questions: 1) What is the sensitivity (power) of SOC data in the current FIA database? 2) How does the minimum detectable change in forest SOC respond to changes in sampling intervals and/or sample point density? Soil samples in the FIA database represent 0-10 cm and 10-20 cm depth increments with a 10-year sampling interval. We are investigating the variability of SOC and its change over time for composite soil data in each FIA region (Pacific Northwest, Interior West, Northern, and Southern). To guide future sampling efforts, we are employing statistical power analysis to examine the minimum detectable change in SOC storage. We are also investigating the sensitivity of SOC storage changes under various scenarios of sample size and/or sample frequency. This research will inform the design of future FIA soil sampling schemes and improve the information available to international policy makers, university and industry partners, and the public.

Improving FIA trend analysis through model-based estimation using landsat disturbance maps and the forest vegetation simulator

Treesearch

Sean P. Healey; Gretchen G. Moisen; Paul L. Patterson

2012-01-01

The Forest Inventory and Analysis (FIA) Program's panel system, in which 10-20 percent of the sample is measured in any given year, is designed to increase the currency of FIA reporting and its sensitivity to factors operating at relatively fine temporal scales. Now that much of the country has completed at least one measurement cycle over all panels, there is an...

A national analytical quality assurance program: Developing guidelines and analytical tools for the forest inventory and analysis program

Treesearch

Phyllis C. Adams; Glenn A. Christensen

2012-01-01

A rigorous quality assurance (QA) process assures that the data and information provided by the Forest Inventory and Analysis (FIA) program meet the highest possible standards of precision, completeness, representativeness, comparability, and accuracy. FIA relies on its analysts to check the final data quality prior to release of a State’s data to the national FIA...

Current status of chestnut in eastern US forests

Treesearch

William H. McWiliams; Tonya W. Lister; Elizabeth B. LaPoint; Anita K. Rose; John S. Vissage

2006-01-01

The USDA Forest Service, Forest Inventory and Analysis (FIA) program provides the opportunity to assess the current distribution of American chestnut (Castanea dentata (Marsh.) Borkh) and prospective trends. Assessing chestnut using the FIA data was challenging because of the coarse nature of the FIA sample and chestnut's rarity in natural...

Spatially Locating FIA Plots from Pixel Values

Treesearch

Greg C. Liknes; Geoffrey R. Holden; Mark D. Nelson; Ronald E. McRoberts

2005-01-01

The USDA Forest Service Forest Inventory and Analysis (FIA) program is required to ensure the confidentiality of the geographic locations of plots. To accommodate user requests for data without releasing actual plot coordinates, FIA creates overlays of plot locations on various geospatial data, including satellite imagery. Methods for reporting pixel values associated...

Symposium: evidence for the use of intra-articular cortisone or hyaluronic acid injection in the hip

PubMed Central

Chandrasekaran, Sivashankar; Lodhia, Parth; Suarez-Ahedo, Carlos; Vemula, S. Pavan; Martin, Timothy J.; Domb, Benjamin G.

2016-01-01

The primary purpose of this review article is to discuss the role of diagnostic, corticosteroid, hyaluronic acid (HA) and platelet rich plasma (PRP) in the treatment of osteoarthritis (OA) and femoroacetabular impingement (FIA). These treatments play an important biological role in the non-operative management of these conditions. Two independent reviewers performed an search of PubMed for articles that contained at least one of the following search terms pertaining to intra-articular hip injection—local anaesthetic, diagnostic, ultrasound, fluoroscopic, image guided, corticosteroid, HA, PRP, OA, labral tears and FAI. Seventy-two full text articles were suitable for inclusion. There were 18 articles addressing the efficacy of diagnostic intra-articular hip injections. With respect to efficacy in OA there were 25 articles pertaining to efficacy of corticosteroid, 22 of HA and 4 of PRP. There were three articles addressing the efficacy of biologics in FAI. Diagnostic intra-articular hip injections are sensitive and specific for differentiating between intra-articular, extra-articular and spinal causes of hip symptoms. Ultrasound and fluoroscopy improves the precision of intra-articular positioning of diagnostic injections. Corticosteroids are more effective than HA and PRP in alleviating pain from hip OA. A higher dose of corticosteroids produces a longer benefit but volume of injection has no significant effect. Intra-articular corticosteroids do not increase infection rates of subsequent arthroplasty. There is currently limited evidence to warrant the routine use of therapeutic injections in the management of labral tears and FIA. PMID:27026814

Alcohol biosensing by polyamidoamine (PAMAM)/cysteamine/alcohol oxidase-modified gold electrode.

PubMed

Akin, Mehriban; Yuksel, Merve; Geyik, Caner; Odaci, Dilek; Bluma, Arne; Höpfner, Tim; Beutel, Sascha; Scheper, Thomas; Timur, Suna

2010-01-01

A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4 degrees C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation. Copyright 2010 American Institute of Chemical Engineers

Improving adjuvant systems for polyclonal egg yolk antibody (IgY) production in laying hens in terms of productivity and animal welfare.

PubMed

Marcq, Christopher; Marlier, Didier; Beckers, Yves

2015-05-15

The antibody production in the egg yolks of immunized laying hens is seen as a way of improving animal welfare compared with conventional production by mammals. Immunoglobulin Y (IgY) technology, however, has still to address welfare issues linked to the widespread use of an adjuvant in vaccines. Currently, Freund's adjuvants, complete (FCA) or incomplete (FIA), remain the standard. This study sought to evaluate various approaches used to enhance egg yolk antibody production in terms of both productivity and avian welfare. The outer membrane protein (OMP) of Salmonella Typhimurium was used as the prototype antigen. At 20 weeks of age, 56 ISA Brown hens, with specific-Salmonella-free status, were divided into seven groups (n=8) and received an initial intramuscular immunization. Hens in the two negative control groups received phosphate buffered saline (PBS) or FIA alone. Hens in the other groups received 80μg of Salmonella OMP emulsified with one of the following adjuvants: 200μl of FIA alone (T1); 200μl of FIA supplemented with 8μg of C-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) (T2); and 280μl of Montanide ISA 70 VG (T4). Birds in the T3 group received the antigen in emulsion with FIA and were given the tested immunostimulatory component (l-carnitine) via their feed (100mg/kg). A positive control group (PC) received FCA for the first and final immunizations and FIA for the other boosters. Immunization was repeated after 20, 46, 82 and 221 days. Eggs were collected regularly until 242 days after the first immunization and the anti-Salmonella Typhimurium activities in the yolk were determined by ELISA. After 242 days, the birds were euthanized and the injection sites were evaluated for gross and microscopic lesions. Among the tested immunostimulatory approaches, supplementation of FIA with CpG-ODN led to a significant and long-lasting enhancement of the specific antibody response. This treatment was even higher than the positive benchmark using FCA in the first immunization. The study results showed that a clinical examination of injection sites is insufficient for drawing conclusions about the local tolerance of vaccines. Tissue damage was noticeable in all treatment groups. The birds receiving the Montanide adjuvant, however, had fewer and less severe lesions. Given these limited side-effects, Montanide ISA 70 VG could provide the depot effect needed to ensure the immunomodulatory efficiency of CpG-ODN. The association of these two adjuvants could prove a promising alternative to Freund's adjuvants (FA). Copyright © 2015 Elsevier B.V. All rights reserved.

Forests of Illinois, 2015

Treesearch

Susan Crocker; Brett Butler

2016-01-01

This publication provides an overview of forest resources in Illinois based on an annual inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. Information about the FIA program is available at...

Coordination, Cooperation, and Collaboration between FIA and NRI

Treesearch

Raymond L. Czaplewski; James Rack; Veronica C. Lessard; David F. Heinzen; Susan Ploetz; Thomas L. Schmidt; Earl C. Leatherberry

2005-01-01

The USDA Forest Service conducts a detailed survey of the Nation's forests through the Forest Inventory and Analysis (FIA) program. The USDA Natural Resources Service conducts an entirely separate survey, the National Resources Inventory (NRI), to monitor status and trends in the Nation's soil and other natural resources. Blue Ribbon Panels for both FIA and...

Forests of Ohio, 2016

Treesearch

Thomas A. Albright

2017-01-01

This resource update provides an overview of the forest resources in Ohio based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly.1 Information about the national and regional FIA...

National FIA plot intensification procedure report

Treesearch

Jock A. Blackard; Paul L. Patterson

2014-01-01

The Forest Inventory and Analysis (FIA) program of the U.S. Forest Service (USFS) measures a spatially distributed base grid of forest inventory plots across the United States. The sampling intensity of plots may be increased in some regions when warranted by specific inventory objectives. Several intensification methods have been developed within FIA and USFS National...

Doing more with the core: Proceedings of the 2017 Forest Inventory and Analysis (FIA) Science Stakeholder Meeting; 2017 October 24- 26; Park City, UT

Treesearch

Sean P. Healey; Vicki M. Berrett

2017-01-01

The Forest Service’s Forest Inventory and Analysis Program (FIA) is the primary source of information about our forests’ status and trends. A network of nationally consistent field observations forms FIA’s core, and active collaboration with clients and peer organizations ensures that the resulting inventory remains agile, comprehensive, and relevant. An FIA Science...

Kalman filter based glucose control at small set points during fed-batch cultivation of Saccharomyces cerevisiae.

PubMed

Arndt, Michael; Hitzmann, Bernd

2004-01-01

A glucose control system is presented, which is able to control cultivations of Saccharomyces cerevisiae even at low glucose concentrations. Glucose concentrations are determined using a special flow injection analysis (FIA) system, which does not require a sampling module. An extended Kalman filter is employed for smoothing the glucose measurements as well as for the prediction of glucose and biomass concentration, the maximum specific growth rate, and the volume of the culture broth. The predicted values are utilized for feedforward/feedback control of the glucose concentration at set points of 0.08 and 0.05 g/L. The controller established well-defined conditions over several hours up to biomass concentrations of 13.5 and 20.7 g/L, respectively. The specific glucose uptake rates at both set points were 1.04 and 0.68 g/g/h, respectively. It is demonstrated that during fed-batch cultivation an overall pure oxidative metabolism of glucose is maintained at the lower set point and a specific ethanol production rate of 0.18 g/g/h at the higher set point.

Changes in Black Carbon Deposition to Antarctica from Two Ice Core Records, A.D. 1850-2000

NASA Technical Reports Server (NTRS)

Bisiaux, Marion M.; Edward, Ross; McConnell, Joseph R.; Curran, Mark A. J.; VanOmmen, Tas D.; Smith, Andrew M.; Neumann, Thomas A.; Pasteris, Daniel R.; Penner, Joyce E.; Taylor, Kendrick

2012-01-01

Continuous flow analysis was based on a steady sample flow and in-line detection of BC and other chemical substances as described in McConnell et al. (2007). In the cold room, previously cut one meter ice core sticks of 3x3cm, are melted continuously on a heated melter head specifically designed to eliminate contamination from the atmosphere or by the external parts of the ice. The melted ice from the most inner part of the ice stick is continuously pumped by a peristaltic pump and carried to a clean lab by Teflon lines. The recorded signal is continuous, integrating a sample volume of about 0.05 mL, for which the temporal resolution depends on the speed of melting, ice density and snow accumulation rate at the ice core drilling site. For annual accumulation derived from the WAIS and Law Dome ice cores, we assumed 3.1 cm water equivalent uncertainty in each year's accumulation from short scale spatial variability (glaciological noise) which was determined from several measurements of annual accumulation in multiple parallel ice cores notably from the WAIS Divide ice core site (Banta et al., 2008) and from South Pole site (McConnell et al., 1997; McConnell et al., 2000). Refractory black carbon (rBC) concentrations were determined using the same method as in (Bisiaux et al., 2011) and adapted to continuous flow measurements as described by (McConnell et al., 2007). The technique uses a single particle intracavity laser induced incandescence photometer (SP2, Droplet Measurement Technologies, Boulder, Colorado) coupled to an ultrasonic nebulizer/desolvation (CETAC UT5000) Flow Injection Analysis (FIA). All analyses, sample preparation etc, were performed in a class 100 cleanroom using anti contamination "clean techniques". The samples were not acidified.

FIA Estimation in the New Millennium

Treesearch

Francis A. Roesch

2001-01-01

In the new millennium, Forest Inventory and Analysis (FIA) will deliver most of its database information directly to the users over the Internet. This assumption indicates the need for a GIS-based estimation system to support the information delivery system. Presumably, as the data set evolves, it will free FIA and the users from exclusive estimation within political...

Overview of the National Inventory and Monitoring Applications Center (NIMAC)

Treesearch

Charles T. Scott

2009-01-01

The National Inventory and Monitoring Applications Center (NIMAC) was created by the Forest Inventory and Analysis (FIA) program in 2006. NIMAC addresses a growing need, expressed by FIA partners, for technical assistance in designing and implementing monitoring plans for forests at scales finer than that provided by the FIA standard inventory. NIMAC's goal is to...

Estimating number and size of forest patches from FIA plot data

Treesearch

Mark D. Nelson; Andrew J. Lister; Mark H. Hansen

2009-01-01

Forest inventory and analysis (FIA) annual plot data provide for estimates of forest area, type, volume, growth, and other attributes. Estimates of forest landscape metrics, such as those describing abundance, size, and shape of forest patches, however, typically are not derived from FIA plot data but from satellite image-based land cover maps. Associating image-based...

The hexagon/panel system for selecting FIA plots under an annual inventory

Treesearch

Gary J. Brand; Mark D. Nelson; Daniel G. Wendt; Kevin K. Nimerfro

2000-01-01

Forest Inventory and Analysis (FIA) is changing to an annual nationwide forest inventory. This paper describes the sampling grid used to distribute FIA plots across the landscape and to allocate them to a particular measurement year. We also describe the integration of the F1A and Forest Health Monitoring (FHM) plot networks.

Forests of Maryland, 2016

Treesearch

Tonya W. Lister

2017-01-01

This publication provides an overview of forest resources in Maryland based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. From 2004-2013, FIA employed an annual inventory measuring data on 20 percent of all sample plots each year in Maryland. Beginning in 2014, FIA is on a 7-year cycle...

Forests of Maryland, 2015

Treesearch

Tonya Lister; Richard Widmann

2016-01-01

This publication provides an overview of forest resources in Maryland based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. From 2004-2013, FIA employed an annual inventory, measuring 20 percent of all sample plots each year in Maryland. Beginning in 2014, FIA is on a 7-year cycle,...

Landsat TM Classifications For SAFIS Using FIA Field Plots

Treesearch

William H. Cooke; Andrew J. Hartsell

2001-01-01

Wall-to-wall Landsat Thematic Mapper (TM) classification efforts in Georgia require field validation. We developed a new crown modeling procedure based on Forest Health Monitoring (FHM) data to test Forest Inventory and Analysis (FIA) data. These models simulate the proportion of tree crowns that reflect light on a FIA subplot basis. We averaged subplot crown...

Forests of New York, 2016

Treesearch

Thomas A. Albright; Anthony C. Olsen

2017-01-01

This resource update provides an overview of the forest resources in New York based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly.1Information about the national and regional FIA...

Forests of Maryland, 2014

Treesearch

T.W. Lister; R.H. Widmann

2015-01-01

This publication provides an overview of forest resources in Maryland based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. From 2004-2013, FIA employed an annual inventory measuring data on 20 percent of all sample plots each year in Maryland. Beginning in 2014, FIA is on a 7-year cycle...

Regression and Geostatistical Techniques: Considerations and Observations from Experiences in NE-FIA

Treesearch

Rachel Riemann; Andrew Lister

2005-01-01

Maps of forest variables improve our understanding of the forest resource by allowing us to view and analyze it spatially. The USDA Forest Service's Northeastern Forest Inventory and Analysis unit (NE-FIA) has used geostatistical techniques, particularly stochastic simulation, to produce maps and spatial data sets of FIA variables. That work underscores the...

Summary of mortality statistics and forest health monitoring results for the Northeastern United States

Treesearch

William H. McWilliams; Stanford L. Arner; Charles J. Barnett

1997-01-01

The USDA Forest Service's Forest Inventory and Analysis (FIA) program and the Forest Health Monitoring (FHM) program maintain networks of sample locations providing coarse-scale information that characterize general indicators of forest health. Tree mortality is the primary FIA variable for analyzing forest health. Recent FIA inventories of New York, Pennsylvania...

Application of Forest Inventory and Analysis (FIA) data to estimate the amount of old growth forest and snag density in the Northern Region of the National Forest System

Treesearch

Raymond L. Czaplewski

2004-01-01

This report discusses valid use of data produced by the Forest Service?s Forest Inventory and Analysis (FIA) program and used by the Northern Region of the National Forest System to analyze the compliance of individual National Forests with their Standards and Guidelines. It emphasizes use of FIA data on snag density and the percentage of forest area that meets the...

Point pattern analysis of FIA data

Treesearch

Chris Woodall

2002-01-01

Point pattern analysis is a branch of spatial statistics that quantifies the spatial distribution of points in two-dimensional space. Point pattern analysis was conducted on stand stem-maps from FIA fixed-radius plots to explore point pattern analysis techniques and to determine the ability of pattern descriptions to describe stand attributes. Results indicate that the...

SOLE: enhanced FIA data analysis capabilities

Treesearch

Michael Spinney; Paul Van Deusen

2009-01-01

The Southern On Line Estimator (SOLE), is an Internet-based annual forest inventory and analysis (FIA) data analysis tool developed cooperatively by the National Council for Air and Stream Improvement and the Forest Service, U.S. Department of Agriculture's Forest Inventory and Analysis program at the Southern Research Station. Recent development of SOLE has...

An evaluation of the properties of the variance estimator used by FIA

Treesearch

John P. Brown; James A. Westfall

2012-01-01

The Forest Inventory and Analysis (FIA) program of the U.S. Forest Service currently conducts inventories utilizing the protocols of the national enhanced FIA Program. Due to the permanent locations of the sample plots, the stratification of the population occurs after the selection of sample units, i.e., post-stratification. In situations where the population is of...

The National Inventory of Down Woody Materials: Methods, Outputs, and Future Directions

Treesearch

Christopher W. Woodall

2003-01-01

The Forest Inventory and Analysis Program (FIA) of the USDA Forest Service conducts a national inventory of forests of the United States. A subset of FIA permanent inventory plots are sampled every year for numerous forest health indicators ranging fiom soils to understory vegetation. Down woody material (DWM) is an FIA indicator that refines estimation of forest...

Estimating and circumventing the effects of perturbing and swapping inventory plot locations

Treesearch

Ronald E. McRoberts; Geoffrey R. Holden; Mark D. Nelson; Greg C. Liknes; Warren K. Moser; Andrew J. Lister; Susan L. King; Elizabeth B. LaPoint; John W. Coulston; W. Brad Smith; Gregory A. Reams

2005-01-01

The Forest Inventory and Analysis (FIA) program of the USDA Forest Service reports data and information about the Nation's forest resources. Increasingly, users request that FIA data and information be reported and distributed in a geospatial context, and they request access to exact plot locations for their own analyses. However, the FIA program is constrained by...

An overview of inventory and monitoring and the Role of FIA in National Assessments

Treesearch

W. Brad Smith

2006-01-01

This paper presents a brief conceptual overview of inventory and monitoring and the role of the Forest Inventory and Analysis (FIA) program in national assessments. FIA has become a focal point of national inventory and monitoring and kept national leadership as well as forest resource research and management professionals apprised, through periodic reports to Congress...

Variable Selection Strategies for Small-area Estimation Using FIA Plots and Remotely Sensed Data

Treesearch

Andrew Lister; Rachel Riemann; James Westfall; Mike Hoppus

2005-01-01

The USDA Forest Service's Forest Inventory and Analysis (FIA) unit maintains a network of tens of thousands of georeferenced forest inventory plots distributed across the United States. Data collected on these plots include direct measurements of tree diameter and height and other variables. We present a technique by which FIA plot data and coregistered...

FIA BioSum: a tool to evaluate financial costs, opportunities and effectiveness of fuel treatments.

Treesearch

Jeremy Fried; Glenn Christensen

2004-01-01

FIA BioSum, a tool developed by the USDA Forest Services Forest Inventory and Analysis (FIA) Program, generates reliable cost estimates, identifies opportunities and evaluates the effectiveness of fuel treatments in forested landscapes. BioSum is an analytic framework that integrates a suite of widely used computer models with a foundation of attribute-rich,...

Northeastern FIA Tree Taper Study: Current Status and Future Work

Treesearch

James A. Westfall; Charles T. Scott

2005-01-01

The northeastern unit of the Forest Inventory and Analysis program (NE-FIA) is engaged in an ongoing project to develop regionwide tree taper equations. Sampling intensity is based on NE-FIA plot data and is stratified by species, diameter class, and height class. To date, modeling research has been aimed largely at evaluating existing model forms (and hybrids thereof...

Development of carbon response trajectories using FIA plot data and FVS growth simulator: challenges of a large scale simulation project

Treesearch

James B. McCarter; Sean Healey

2015-01-01

The Forest Carbon Management Framework (ForCaMF) integrates Forest Inventory and Analysis (FIA) plot inventory data, disturbance histories, and carbon response trajectories to develop estimates of disturbance and management effects on carbon pools for the National Forest System. All appropriate FIA inventory plots are simulated using the Forest Vegetation Simulator (...

Phase 2 and phase 3 presentation grids

Treesearch

Joseph McCollum; Jamie K. Cochran

2009-01-01

Many forest inventory and analysis (FIA) analysts, other researchers, and FIA Spatial Data Services personnel have expressed their desire to use the FIA Phase 2 (P2) and Phase 3 (P3), and Forest Health Monitoring (FHM) grids in presentations and other analytical reports. Such uses have been prohibited due to the necessity of keeping the actual P2, P3, and FHM grids...

Comparison of Programs Used for FIA Inventory Information Dissemination and Spatial Representation

Treesearch

Roger C. Lowe; Chris J. Cieszewski

2005-01-01

Six online applications developed for the interactive display of Forest Inventory and Analysis (FIA) data in which FIA database information and query results can be viewed as or selected from interactive geographic maps are compared. The programs evaluated are the U.S. Department of Agriculture Forest Service?s online systems; a SAS server-based mapping system...

Calibration of the STEMS diameter growth model using FIA data

Treesearch

Veronica C. Lessard

2000-01-01

The diameter growth model used in STEMS, the Stand and Tree Evaluation and Modeling System, was originally calibrated using data from permanent growth plots in Minnesota, Wisconsin, and Michigan. Because the model has been applied in predicting growth using Forest Inventory and Analysis (FIA) data, it was appropriate to refit the model to FIA data. The model was...

How to do (or not to do) … a health financing incidence analysis

PubMed Central

Asante, Augustine D; Limwattananon, Supon; Wiseman, Virginia

2018-01-01

Abstract Financing incidence analysis (FIA) assesses how the burden of health financing is distributed in relation to household ability to pay (ATP). In a progressive financing system, poorer households contribute a smaller proportion of their ATP to finance health services compared to richer households. A system is regressive when the poor contribute proportionately more. Equitable health financing is often associated with progressivity. To conduct a comprehensive FIA, detailed household survey data containing reliable information on both a cardinal measure of household ATP and variables for extracting contributions to health services via taxes, health insurance and out-of-pocket (OOP) payments are required. Further, data on health financing mix are needed to assess overall FIA. Two major approaches to conducting FIA described in this article include the structural progressivity approach that assesses how the share of ATP (e.g. income) spent on health services varies by quantiles, and the effective progressivity approach that uses indices of progressivity such as the Kakwani index. This article provides some detailed practical steps for analysts to conduct FIA. This includes the data requirements, data sources, how to extract or estimate health payments from survey data and the methods for assessing FIA. It also discusses data deficiencies that are common in many low- and middle-income countries (LMICs). The results of FIA are useful in designing policies to achieve an equitable health system. PMID:29346547

How to do (or not to do) … a health financing incidence analysis.

PubMed

Ataguba, John E; Asante, Augustine D; Limwattananon, Supon; Wiseman, Virginia

2018-04-01

Financing incidence analysis (FIA) assesses how the burden of health financing is distributed in relation to household ability to pay (ATP). In a progressive financing system, poorer households contribute a smaller proportion of their ATP to finance health services compared to richer households. A system is regressive when the poor contribute proportionately more. Equitable health financing is often associated with progressivity. To conduct a comprehensive FIA, detailed household survey data containing reliable information on both a cardinal measure of household ATP and variables for extracting contributions to health services via taxes, health insurance and out-of-pocket (OOP) payments are required. Further, data on health financing mix are needed to assess overall FIA. Two major approaches to conducting FIA described in this article include the structural progressivity approach that assesses how the share of ATP (e.g. income) spent on health services varies by quantiles, and the effective progressivity approach that uses indices of progressivity such as the Kakwani index. This article provides some detailed practical steps for analysts to conduct FIA. This includes the data requirements, data sources, how to extract or estimate health payments from survey data and the methods for assessing FIA. It also discusses data deficiencies that are common in many low- and middle-income countries (LMICs). The results of FIA are useful in designing policies to achieve an equitable health system.

Flow injection analysis of ethyl xanthate by gas diffusion and UV detection as CS2 for process monitoring of sulfide ore flotation.

PubMed

Cordeiro, Thiago G; Hidalgo, Pilar; Gutz, Ivano G R; Pedrotti, Jairo J

2010-07-15

A sensitive and robust analytical method for spectrophotometric determination of ethyl xanthate, CH(3)CH(2)OCS(2)(-) at trace concentrations in pulp solutions from froth flotation process is proposed. The analytical method is based on the decomposition of ethyl xanthate, EtX(-), with 2.0 mol L(-1) HCl generating ethanol and carbon disulfide, CS(2). A gas diffusion cell assures that only the volatile compounds diffuse through a PTFE membrane towards an acceptor stream of deionized water, thus avoiding the interferences of non-volatile compounds and suspended particles. The CS(2) is selectively detected by UV absorbance at 206 nm (epsilon=65,000 L mol(-1) cm(-1)). The measured absorbance is directly proportional to EtX(-) concentration present in the sample solutions. The Beer's law is obeyed in a 1x10(-6) to 2x10(-4) mol L(-1) concentration range of ethyl xanthate in the pulp with an excellent correlation coefficient (r=0.999) and a detection limit of 3.1x10(-7) mol L(-1), corresponding to 38 microg L(-1). At flow rates of 200 microL min(-1) of the donor stream and 100 microL min(-1) of the acceptor channel a sampling rate of 15 injections per hour could be achieved with RSD<2.3% (n=10, 300 microL injections of 1x10(-5) mol L(-1) EtX(-)). Two practical applications demonstrate the versatility of the FIA method: (i) evaluation the free EtX(-) concentration during a laboratory study of the EtX(-) adsorption capacity on pulverized sulfide ore (pyrite) and (ii) monitoring of EtX(-) at different stages (from starting load to washing effluents) of a flotation pilot plant processing a Cu-Zn sulfide ore. Copyright 2010 Elsevier B.V. All rights reserved.

On-Line Analysis of Southern FIA Data

Treesearch

Michael P. Spinney; Paul C. Van Deusen; Francis A. Roesch

2006-01-01

The Southern On-Line Estimator (SOLE) is a web-based FIA database analysis tool designed with an emphasis on modularity. The Java-based user interface is simple and intuitive to use and the R-based analysis engine is fast and stable. Each component of the program (data retrieval, statistical analysis and output) can be individually modified to accommodate major...

Extending and Intensifying the FIA Inventory of Down Forest Fuels: Boundary Waters Canoe Area and Pictured Rocks National Lakeshore

Treesearch

Christopher W. Woodall; Bruce Leutscher

2005-01-01

The sampling design for the Forest Inventory and Analysis (FIA) program of the U.S. Department of Agriculture Forest Service allows intensification of fuel inventory sampling in areas of ?special interest? and implementation of fuel sampling protocol by non-FIA personnel. The objective of this study is to evaluate the contribution of sampling intensification/extension...

A GIS-based tool for estimating tree canopy cover on fixed-radius plots using high-resolution aerial imagery

Treesearch

Sara A. Goeking; Greg C. Liknes; Erik Lindblom; John Chase; Dennis M. Jacobs; Robert. Benton

2012-01-01

Recent changes to the Forest Inventory and Analysis (FIA) Program's definition of forest land precipitated the development of a geographic information system (GIS)-based tool for efficiently estimating tree canopy cover for all FIA plots. The FIA definition of forest land has shifted from a density-related criterion based on stocking to a 10 percent tree canopy...

A diameter growth model for the SRS FIA

Treesearch

David Gartner

2015-01-01

Changes in the national Forest Inventory and Analysis (FIA) processing system required the Southern Research Station’s FIA unit to create a diameter growth model to estimate the growth of trees that could not be measured at both ends of a measurement interval. Examples of such trees are trees that have died or been harvested, and trees that grow over the minimum...

Austin's urban FIA: seamless rural to urban resource monitoring in Texas

Treesearch

Chris Edgar; Burl Carraway

2015-01-01

In 2014 Urban Forest Inventory and Analysis (Urban-FIA) was implemented for the first time ever in Austin, Texas. Work was accelerated and a full complement of plots in the city was measured in six months. In 2015 results are to be released in an FIA report and data made available in a publicly accessible database. In this presentation we discuss the importance of...

A density management diagram for even-aged ponderosa pine stands

Treesearch

James N. Long; John D. Shaw

2005-01-01

We developed a density management diagram (DMD) for ponderosa pine using Forest Inventory and Analysis (FIA) data. Analysis plots were drawn from all FIA plots in the western United States on which ponderosa pine occurred. A total of 766 plots met the criteria for analysis. Selection criteria were for purity, defined as ponderosa pine basal area 80% of plot basal area...

Analyzing lichen indicator data in the Forest Inventory and Analysis Program

Treesearch

Susan Will-Wolf

2010-01-01

Lichens are one of several forest health indicators sampled every year for a subset of plots on the permanent grid established by the Forest Inventory and Analysis (FIA) Program of the U.S. Department of Agriculture Forest Service. This report reviews analysis procedures for standard FIA lichen indicator data. Analyses of lichen data contribute to state, regional, and...

Statistics and quality assurance for the Northern Research Station Forest Inventory and Analysis Program, 2016

Treesearch

Dale D. Gormanson; Scott A. Pugh; Charles J. Barnett; Patrick D. Miles; Randall S. Morin; Paul A. Sowers; Jim Westfall

2017-01-01

The U.S. Forest Service Forest Inventory and Analysis (FIA) program collects sample plot data on all forest ownerships across the United States. FIA's primary objective is to determine the extent, condition, volume, growth, and use of trees on the Nation's forest land through a comprehensive inventory and analysis of the Nation's forest resources. The...

An Analysis of Losses to the Southern Commercial Timberland Base

Treesearch

Ian A. Munn; David Cleaves

1998-01-01

Demographic and physical factors influencing the conversion of commercial timberland iu the south to non-forestry uses between the last two Forest Inventory Analysis (FIA) surveys were investigated. GIS techniques linked Census data and FIA plot level data. Multinomial logit regression identified factors associated with losses to the timberland base. Conversion to...

Mississippi, 2012 forest inventory and analysis factsheet

Treesearch

Sonja N. Oswalt

2013-01-01

This science update provides an overview of forest resources in Mississippi based on an inventory conducted by the U.S. Department of Agriculture Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Mississippi Forestry Commission. Data estimates are based on field data collected using the FIA annualized...

Mississippi, 2011 forest inventory and analysis factsheet

Treesearch

Sonja N. Oswalt

2013-01-01

This science update provides an overview of forest resources in Mississippi based on an inventory conducted by the U.S. Department of Agriculture Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Mississippi Forestry Commission. Data estimates are based on field data collected using the FIA annualized...

Inventory-based landscape-scale simulation of management effectiveness and economic feasibility with BioSum

Treesearch

Jeremy S. Fried; Larry D. Potts; Sara M. Loreno; Glenn A. Christensen; R. Jamie Barbour

2017-01-01

The Forest Inventory and Analysis (FIA)-based BioSum (Bioregional Inventory Originated Simulation Under Management) is a free policy analysis framework and workflow management software solution. It addresses complex management questions concerning forest health and vulnerability for large, multimillion acre, multiowner landscapes using FIA plot data as the initial...

FIESTA—An R estimation tool for FIA analysts

Treesearch

Tracey S. Frescino; Paul L. Patterson; Gretchen G. Moisen; Elizabeth A. Freeman

2015-01-01

FIESTA (Forest Inventory ESTimation for Analysis) is a user-friendly R package that was originally developed to support the production of estimates consistent with current tools available for the Forest Inventory and Analysis (FIA) National Program, such as FIDO (Forest Inventory Data Online) and EVALIDator. FIESTA provides an alternative data retrieval and reporting...

Impulse radio ultra wideband wireless transmission of dopamine concentration levels recorded by fast-scan cyclic voltammetry.

PubMed

Ebrazeh, Ali; Bozorgzadeh, Bardia; Mohseni, Pedram

2015-01-01

This paper demonstrates the feasibility of utilizing impulse radio ultra wideband (IR-UWB) signaling technique for reliable, wireless transmission of dopamine concentration levels recorded by fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) to address the problem of elevated data rates in high-channel-count neurochemical monitoring. Utilizing an FSCV-sensing chip fabricated in AMS 0.35μm 2P/4M CMOS, a 3-5-GHz, IR-UWB transceiver (TRX) chip fabricated in TSMC 90nm 1P/9M RF CMOS, and two off-chip, miniature, UWB antennae, wireless transfer of pseudo-random binary sequence (PRBS) data at 50Mbps over a distance of <;1m is first shown with bit-error rates (BER) <; 10(-3). Further, IR-UWB wireless transmission of dopamine concentration levels prerecorded with FSCV at a CFM during flow injection analysis (FIA) is also demonstrated with transmitter (TX) power dissipation of only ~4.4μW from 1.2V, representing two orders of magnitude reduction in TX power consumption compared to that of a conventional frequency-shift-keyed (FSK) link operating at ~433MHz.

Repeated measures from FIA data facilitates analysis across spatial scales of tree growth responses to nitrogen deposition from individual trees to whole ecoregions

Treesearch

Charles H. (Hobie) Perry; Kevin J. Horn; R. Quinn Thomas; Linda H. Pardo; Erica A.H. Smithwick; Doug Baldwin; Gregory B. Lawrence; Scott W. Bailey; Sabine Braun; Christopher M. Clark; Mark Fenn; Annika Nordin; Jennifer N. Phelan; Paul G. Schaberg; Sam St. Clair; Richard Warby; Shaun Watmough; Steven S. Perakis

2015-01-01

The abundance of temporally and spatially consistent Forest Inventory and Analysis data facilitates hierarchical/multilevel analysis to investigate factors affecting tree growth, scaling from plot-level to continental scales. Herein we use FIA tree and soil inventories in conjunction with various spatial climate and soils data to estimate species-specific responses of...

Oklahoma, 2011 - forest inventory and analysis factsheet

Treesearch

Jason A. Cooper

2013-01-01

This science update summarizes the findings of the statewide annual inventory of the forest resource attributes in Oklahoma conducted by the Southern Forest Inventory and Analysis (FIA) program in cooperation with the Oklahoma Forestry Services. The 77 counties of Oklahoma are consolidated into seven FIA survey units— southeast (unit 1), northeast (unit 2), north...

Improving Forest Inventory and Analysis efficiency with common land unit information

Treesearch

Greg C. Liknes; Mark D. Nelson

2009-01-01

The Forest Service, U.S. Department of Agriculture's (USDA's) Northern Research Station Forest Inventory and Analysis program (NRS-FIA) examines inventory locations on digital aerial imagery to determine if the land use at each plot location meets the FIA definition of forest and thereby becomes a field visit site. This manual image-interpretation effort...

Forest Inventory and Analysis and Forest Health Monitoring: Piecing the Quilt

Treesearch

Joseph M. McCollum; Jamie K. Cochran

2005-01-01

Against the backdrop of a discussion about patchwork quilt assembly, the authors present background information on global grids. They show how to compose hexagons, an important task in systematically developing a subset of Forest Health Monitoring (FHM) Program plots from Forest Inventory and Analysis (FIA) plots. Finally, they outline the FHM and FIA grids, along with...

Using classified Landsat Thematic Mapper data for stratification in a statewide forest inventory

Treesearch

Mark H. Hansen; Daniel G. Wendt

2000-01-01

The 1998 Indiana/Illinois forest inventory (USDA Forest Service, Forest Inventory and Analysis (FIA)) used Landsat Thematic Mapper (TM) data for stratification. Classified images made by the National Gap Analysis Program (GAP) stratified FIA plots into four classes (nonforest, nonforest/ forest, forest/nonforest, and forest) based on a two pixel forest edge buffer zone...

Using Classified Landsat Thematic Mapper Data for Stratification in a Statewide Forest Inventory

Treesearch

Mark H. Hansen; Daniel G. Wendt

2000-01-01

The 1998 Indiana/Illinois forest inventory (USDA Forest Service, Forest Inventory and Analysis (FIA)) used Landsat Thematic Mapper (TM} data for stratification. Classified images made by the National Gap Analysis Program (GAP) stratified FIA plots into four classes (nonforest, nonforest/forest, forest/nonforest, and forest) based on a two pixel forest edge buffer zone...

A primer of nonresponse in the US Forest Inventory and Analysis program

Treesearch

Paul L. Patterson; John W. Coulston; Francis A. Roesch; James A. Westfall; Andrew D. Hill

2012-01-01

Nonresponse caused by denied access and hazardous conditions are a concern for the USDA Forest Service, Forest Inventory and Analysis (FIA) program, whose mission is to quantify status and trends in forest resources across the USA. Any appreciable amount of nonresponse can cause bias in FIA's estimates of population parameters. This paper will quantify the...

Mapping Forest Inventory and Analysis forest land use: timberland, reserved forest land, and other forest land

Treesearch

Mark D. Nelson; John Vissage

2007-01-01

The Forest Inventory and Analysis (FIA) program produces area estimates of forest land use within three subcategories: timberland, reserved forest land, and other forest land. Mapping these subcategories of forest land requires the ability to spatially distinguish productive from unproductive land, and reserved from nonreserved land. FIA field data were spatially...

Predicting bird habitat quality from a geospatial analysis of FIA data

Treesearch

John M. Tirpak; D. Todd Jones-Farrand; Frank R., III Thompson; Daniel J. Twedt; Mark D. Nelson; William B., III Uihlein

2009-01-01

The ability to assess the influence of site-scale forest structure on avian habitat suitability at an ecoregional scale remains a major methodological constraint to effective biological planning for forest land birds in North America. We evaluated the feasibility of using forest inventory and analysis (FIA) data to define vegetation structure within forest patches,...

Challenges of working with FIADB17 data: the SOLE experience

Treesearch

Michael Spinney; Paul Van Deusen

2007-01-01

The Southern On Line Estimator (SOLE) is an Internet-based Forest Inventory and Analysis (FIA) data analysis tool. SOLE is based on data downloaded from the publicly available FIA database (FIADB) and summarized by plot condition. The tasks of downloading, processing, and summarizing FIADB data require specialized expertise in inventory theory and data manipulation....

South Carolina, 2010 forest inventory and analysis factsheet

Treesearch

Roger C. Conner

2011-01-01

The Forest Inventory and Analysis (FIA) Program implemented a nationally consistent annual inventory system in 1998. Under the new design, one-fifth of all inventory plots in South Carolina are visited each year. The southern FIA unit, working cooperatively with South Carolina Forestry Commission crews, established the State’s initial annual inventory plots during the...

The virtual analyst program: automated data mining, error analysis, and reporting

Treesearch

W. Keith Moser; Mark H. Hansen; Patrick Miles; Ronald E. McRoberts

2007-01-01

The Forest Inventory and Analysis (FIA) program of the U.S. Department of Agriculture Forest Service conducts ongoing comprehensive inventories of the forest resources of the United States. The Northern Region FIA (NFIA) program has three tasks: (1) core reporting function, which produces the annual and 5-year inventory reports; (2) forest health measurements; and (3)...

Building the Forest Inventory and Analysis Tree-Ring Data set

Treesearch

Robert J. DeRose; John D. Shaw; James N. Long

2017-01-01

The Interior West Forest Inventory and Analysis (IW-FIA) program measures forestland conditions at great extent with relatively high spatial resolution, including the collection of tree-ring data. We describe the development of an unprecedented spatial tree-ring data set for the IW-FIA that enhances the baseline plot data by incorporating ring-width increment measured...

Texas, 2008 forest inventory and analysis factsheet

Treesearch

James Bentley

2011-01-01

This science update summarizes the findings of the first statewide annual inventory conducted by the Southern Forest Inventory and Analysis (FIA) Program in cooperation with the Texas Forest Service of the forest resource attributes in Texas. The 254 counties of Texas are consolidated into seven FIA survey units—southeast (unit 1), the northeast (unit 2), the north...

FIA: An Open Forensic Integration Architecture for Composing Digital Evidence

NASA Astrophysics Data System (ADS)

Raghavan, Sriram; Clark, Andrew; Mohay, George

The analysis and value of digital evidence in an investigation has been the domain of discourse in the digital forensic community for several years. While many works have considered different approaches to model digital evidence, a comprehensive understanding of the process of merging different evidence items recovered during a forensic analysis is still a distant dream. With the advent of modern technologies, pro-active measures are integral to keeping abreast of all forms of cyber crimes and attacks. This paper motivates the need to formalize the process of analyzing digital evidence from multiple sources simultaneously. In this paper, we present the forensic integration architecture (FIA) which provides a framework for abstracting the evidence source and storage format information from digital evidence and explores the concept of integrating evidence information from multiple sources. The FIA architecture identifies evidence information from multiple sources that enables an investigator to build theories to reconstruct the past. FIA is hierarchically composed of multiple layers and adopts a technology independent approach. FIA is also open and extensible making it simple to adapt to technological changes. We present a case study using a hypothetical car theft case to demonstrate the concepts and illustrate the value it brings into the field.

Average Stand Age from Forest Inventory Plots Does Not Describe Historical Fire Regimes in Ponderosa Pine and Mixed-Conifer Forests of Western North America.

PubMed

Stevens, Jens T; Safford, Hugh D; North, Malcolm P; Fried, Jeremy S; Gray, Andrew N; Brown, Peter M; Dolanc, Christopher R; Dobrowski, Solomon Z; Falk, Donald A; Farris, Calvin A; Franklin, Jerry F; Fulé, Peter Z; Hagmann, R Keala; Knapp, Eric E; Miller, Jay D; Smith, Douglas F; Swetnam, Thomas W; Taylor, Alan H

Quantifying historical fire regimes provides important information for managing contemporary forests. Historical fire frequency and severity can be estimated using several methods; each method has strengths and weaknesses and presents challenges for interpretation and verification. Recent efforts to quantify the timing of historical high-severity fire events in forests of western North America have assumed that the "stand age" variable from the US Forest Service Forest Inventory and Analysis (FIA) program reflects the timing of historical high-severity (i.e. stand-replacing) fire in ponderosa pine and mixed-conifer forests. To test this assumption, we re-analyze the dataset used in a previous analysis, and compare information from fire history records with information from co-located FIA plots. We demonstrate that 1) the FIA stand age variable does not reflect the large range of individual tree ages in the FIA plots: older trees comprised more than 10% of pre-stand age basal area in 58% of plots analyzed and more than 30% of pre-stand age basal area in 32% of plots, and 2) recruitment events are not necessarily related to high-severity fire occurrence. Because the FIA stand age variable is estimated from a sample of tree ages within the tree size class containing a plurality of canopy trees in the plot, it does not necessarily include the oldest trees, especially in uneven-aged stands. Thus, the FIA stand age variable does not indicate whether the trees in the predominant size class established in response to severe fire, or established during the absence of fire. FIA stand age was not designed to measure the time since a stand-replacing disturbance. Quantification of historical "mixed-severity" fire regimes must be explicit about the spatial scale of high-severity fire effects, which is not possible using FIA stand age data.

Average Stand Age from Forest Inventory Plots Does Not Describe Historical Fire Regimes in Ponderosa Pine and Mixed-Conifer Forests of Western North America

PubMed Central

Stevens, Jens T.; Safford, Hugh D.; North, Malcolm P.; Fried, Jeremy S.; Gray, Andrew N.; Brown, Peter M.; Dolanc, Christopher R.; Dobrowski, Solomon Z.; Falk, Donald A.; Farris, Calvin A.; Franklin, Jerry F.; Fulé, Peter Z.; Hagmann, R. Keala; Knapp, Eric E.; Miller, Jay D.; Smith, Douglas F.; Swetnam, Thomas W.; Taylor, Alan H.

2016-01-01

Quantifying historical fire regimes provides important information for managing contemporary forests. Historical fire frequency and severity can be estimated using several methods; each method has strengths and weaknesses and presents challenges for interpretation and verification. Recent efforts to quantify the timing of historical high-severity fire events in forests of western North America have assumed that the “stand age” variable from the US Forest Service Forest Inventory and Analysis (FIA) program reflects the timing of historical high-severity (i.e. stand-replacing) fire in ponderosa pine and mixed-conifer forests. To test this assumption, we re-analyze the dataset used in a previous analysis, and compare information from fire history records with information from co-located FIA plots. We demonstrate that 1) the FIA stand age variable does not reflect the large range of individual tree ages in the FIA plots: older trees comprised more than 10% of pre-stand age basal area in 58% of plots analyzed and more than 30% of pre-stand age basal area in 32% of plots, and 2) recruitment events are not necessarily related to high-severity fire occurrence. Because the FIA stand age variable is estimated from a sample of tree ages within the tree size class containing a plurality of canopy trees in the plot, it does not necessarily include the oldest trees, especially in uneven-aged stands. Thus, the FIA stand age variable does not indicate whether the trees in the predominant size class established in response to severe fire, or established during the absence of fire. FIA stand age was not designed to measure the time since a stand-replacing disturbance. Quantification of historical “mixed-severity” fire regimes must be explicit about the spatial scale of high-severity fire effects, which is not possible using FIA stand age data. PMID:27196621

Puerto Rico's forest inventory: adapting the forest inventory and analysis program to a Caribbean island.

Treesearch

T. J. Brandeis

2003-01-01

Rapid Changes in vegetation over short distances, high species diversity, and fragmented landscape challege the implementation of the Forest service's Forest inventory and Analysis (FIA)program on Puerto Rico. Applying the hexagonal FIA grid as used on the continental United States, the Forest service is installing a new forest sampling and monitoring framework...

D.B.H. and Survival Analysis: A New Methodology for Assessing Forest Inventory Mortality

Treesearch

Christopher W. Woodall; Patricia L. Grambsch; William Thomas

2005-01-01

Tree mortality has typically been assessed in Forest Inventory and Analysis (FIA) studies through summaries of mortality by location, species, and causal agents. Although these methods have historically been used for most of FIA's tree mortality analyses, they are inadequate for robust assessment of mortality trends and dynamics. To offer a new method of analyzing...

East Texas, 2011 forest inventory and analysis factsheet

Treesearch

Jason A. Cooper; James W. Bentley

2012-01-01

This science update summarizes the findings of the annual inventory conducted by the Southern Forest Inventory and Analysis (FIA) Program in cooperation with the Texas Forest Service of the forest resource attributes in east Texas. The 254 counties of Texas are consolidated into 7 FIA survey units—southeast (unit 1), northeast (unit 2), north central (unit 3), south (...

A density management diagram for even-aged Sierra Nevada mixed-conifer stands

Treesearch

James N. Long; John D. Shaw

2012-01-01

We have developed a density management diagram (DMD) for even-aged mixed-conifer stands in the Sierra Nevada Mountains using forest inventory and analysis (FIA) data. Analysis plots were drawn from FIA plots in California, southern Oregon, and western Nevada which included those conifer species associated with the mixed-conifer forest type. A total of 204 plots met the...

A Guide to nonnative invasive plants inventoried in the north by Forest Inventory and Analysis

Treesearch

Cassandra Olson; Anita F. Cholewa

2009-01-01

The Forest Inventory and Analysis (FIA) program of the U.S. Forest Service is an ongoing endeavor mandated by Congress to determine the extent, condition, volume, growth, and depletions of timber on the Nation's forest land. FIA has responded to a growing demand for other information about our forests including, but not limited to,...

Using Forest Service forest inventory and analysis data to estimate regional oak decline and oak mortality

Treesearch

Kathryn W. Kromroy; Jennifer Juzwik; Paul Castillo; Mark H. Hansen

2008-01-01

Damage and mortality data are collected as part of the US Forest Service, Forest Inventory and Analysis (FIA) ongoing assessments of the nation's timberlands. The usefulness and value of FIA tree data in assessing historical levels of oak decline and oak mortality were investigated for seven Midwestern states. The data were collected during two periodic...

Descriptive statistics of tree crown condition in the Southern United States and impacts on data analysis and interpretation

Treesearch

KaDonna C. Randolph

2006-01-01

The U.S. Department of Agriculture Forest Service, Forest Inventory and Analysis Program (FIA) utilizes visual assessments of tree crown condition to monitor changes and trends in forest health. This report describes and discusses distributions of three FIA crown condition indicators (crown density, crown dieback, and foliage transparency) for trees in the Southern...

East Texas, 2012—Forest Inventory and Analysis Factsheet

Treesearch

Thomas J. Brandeis; Jason A. Cooper; James W. Bentley

2014-01-01

This science update summarizes the findings of the statewide annual inventory of the forest resource attributes in Texas conducted by the Southern Forest Inventory and Analysis (FIA) Program in cooperation with the Texas A&M Forest Service. The 254 counties of Texas are consolidated into seven FIA survey units—southeast (unit 1), northeast (unit 2), north central (...

Forest inventory and analysis data for FVS modelers

Treesearch

Patrick D. Miles

2008-01-01

The USDA Forest Service, Forest Inventory and Analysis (FIA) program has been in continuous operation for over 70 years. FIA’s primary objective is to determine the extent, condition, volume, growth, and depletion of timber on the Nation’s forest land. To accomplish this objective, FIA collects sample plot information on all ownerships across the United States.

A stem-map model for predicting tree canopy cover of Forest Inventory and Analysis (FIA) plots

Treesearch

Chris Toney; John D. Shaw; Mark D. Nelson

2009-01-01

Tree canopy cover is an important stand characteristic that affects understory light, fuel moisture, decomposition rates, wind speed, and wildlife habitat. Canopy cover also is a component of most definitions of forest land used by US and international agencies. The USDA Forest Service Forest Inventory and Analysis (FIA) Program currently does not provide a national...

Forest Inventory and Analysis National Data Quality Assessment Report for 2000 to 2003

Treesearch

James E. Pollard; James A. Westfall; Paul L. Patterson; David L. Gartner; Mark Hansen; Olaf Kuegler

2006-01-01

The Forest Inventory and Analysis program (FIA) is the key USDA Forest Service (USFS) program that provides the information needed to assess the status and trends in the environmental quality of the Nation's forests. The goal of the FIA Quality Assurance (QA) program is to provide a framework to assure the production of complete, accurate and unbiased forest...

Model-based time-series analysis of FIA panel data absent re-measurements

Treesearch

Raymond L. Czaplewski; Mike T. Thompson

2013-01-01

An epidemic of lodgepole pine (Pinus contorta) mortality from the mountain pine beetle (Dendroctonus ponderosae) has swept across the Interior West. Aerial surveys monitor the areal extent of the epidemic, but only Forest Inventory and Analysis (FIA) field data support a detailed assessment at the tree level. Dynamics of the lodgepole pine population occur at a more...

Understory vegetation data quality assessment for the Interior West Forest and Inventory Analysis program

Treesearch

Paul L. Patterson; Renee A. O' Brien

2011-01-01

The Interior West Forest Inventory and Analysis (IW-FIA) program of the USDA Forest Service collects field data on understory vegetation structure that have broad applications. In IW-FIA one aspect of quality assurance is assessed based on the repeatability of field measurements. The understory vegetation protocol consists of two suites of measurements; (1) the...

A profile of Wisconsin's private forest landowers

Treesearch

Earl C. Leatherberry

2000-01-01

In 1997, the USDA Forest Service, & Forest Inventory and Analysis (FIA) Program at the North Central Research Station in St. Paul, Minnesota contacted approximately 3,000 private forest landowners in Wisconsin as part of acomprehensive statewide survey. FIA routinely collects and analyzes forest resources data.

Statistics and quality assurance for the Northern Research Station Forest Inventory and Analysis Program

Treesearch

Dale D. Gormanson; Scott A. Pugh; Charles J. Barnett; Patrick D. Miles; Randall S. Morin; Paul A. Sowers; James A. Westfall

2018-01-01

The U.S. Forest Service Forest Inventory and Analysis (FIA) program collects sample plot data on all forest ownerships across the United States. FIA’s primary objective is to determine the extent, condition, volume, growth, and use of trees on the Nation’s forest land through a comprehensive inventory and analysis of the Nation’s forest resources. The FIA program...

FIA data and species diversity—successes and failures using multivariate analysis techniques, spatial lag and error models and hot-spot analysis

Treesearch

Andrew J. Hartsell

2015-01-01

This study will investigate how global and local predictors differ with varying spatial scale in relation to species evenness and richness in the gulf coastal plain. Particularly, all-live trees >= one-inch d.b.h. Forest Inventory and Analysis (FIA) data was used as the basis for the study. Watersheds are defined by the USGS 12 digit hydrologic units. The...

Removal of copper and iron by polyurethane foam column in FIA system for the determination of nickel in pierced ring.

PubMed

Vongboot, Monnapat; Suesoonthon, Monrudee

2015-01-01

Polyurethane foam (PUF) mini-column was used to eliminate copper and iron for the determination of nickel in pierced rings. The PUF mini-column was connected to FIA system for on-line sorption of copper and iron in complexes form of CuSCN(+) and FeSCN(2+). For this season, the acid solution containing a mixture of Ni(II), Fe(III), Cu(II) and SCN(-) ions was firstly flew into the PUF column. Then, the percolated solution which Fe(III) and Cu(II) ions is separated from analysis was injected into FIA system to react with 4-(2-pyridylazo) resorcinol (PAR) reagent in basic condition which this method is called pH gradient technique. The Ni-PAR complexes obtained were measured theirs absorbance at 500 nm by UV visible spectrophotometer. In this study, it was found that Cu(II) and Fe(III) were completely to form complexes with 400 mmol/L KSCN and entirely to eliminate in acidic condition at pH 3.0. In the optimum condition of these experiments, the method provided the linear relationship between absorbance and the concentration of Ni(II) in the range from 5.00 to 30.00 mg/L. Linear equation is y=0.0134x+0.0033 (R(2)=0.9948). Precision, assessed in the term of the relative standard deviation, RSD, and accuracy for multiple determinations obtained in values of 0.77-1.73% and 97.4%, respectively. The level of an average amount of Ni(II) in six piercing rings was evaluated to be 14.78 mg/g. Copyright © 2014 Elsevier B.V. All rights reserved.

Data bases for forest inventory in the North-Central Region.

Treesearch

Jerold T. Hahn; Mark H. Hansen

1985-01-01

Describes the data collected by the Forest Inventory and Analysis (FIA) Research Work Unit at the North Central Forest Experiment Station. Explains how interested parties may obtain information from the databases either through direct access or by special requests to the FIA database manager.

A profile of Wisconsin''s private forest landowners

Treesearch

Earl C. Leatherberry

2001-01-01

In 1997, the USDA Forest Service, Forest Inventory and Analysis (FIA) Program at the North Central Research Station in St. Paul, Minnesota contacted approximately 3,000 private forest landowners in Wisconsin as part of a comprehensive statewide survey. FIA routinely collects and analyzes forest resources data.

Disentangling forest change from forest inventory change: A case study from the US Interior West

Treesearch

Sara A. Goeking

2015-01-01

Long-term trends in forest attributes are typically assessed using strategic inventories such as the US Department of Agriculture (USDA) Forest Service’s Forest Inventory and Analysis (FIA) program. The implicit assumption of any trend analysis is that data are comparable over time. The 1998 Farm Bill tasked FIA with implementing nationally consistent protocols,...

Volume equations for the Northern Research Station's Forest Inventory and Analysis Program as of 2010

Treesearch

Patrick D. Miles; Andrew D. Hill

2010-01-01

The U.S. Forest Service's Forest Inventory and Analysis (FIA) program collects sample plot data on all forest ownerships across the United States. This report documents the methodology used to estimate live-tree gross, net, and sound volume for the 24 States inventoried by the Northern Research Station's (NRS) FIA unit. Sound volume is of particular interest...

Use of ancillary data to improve the analysis of forest health indicators

Treesearch

Dave Gartner

2013-01-01

In addition to its standard suite of mensuration variables, the Forest Inventory and Analysis (FIA) program of the U.S. Forest Service also collects data on forest health variables formerly measured by the Forest Health Monitoring program. FIA obtains forest health information on a subset of the base sample plots. Due to the sample size differences, the two sets of...

Using Forest Inventory and Analysis data to model plant-climate relationships

Treesearch

Nicholas L. Crookston; Gerald E. Rehfeldt; Marcus V. Warwell

2007-01-01

Forest Inventory and Analysis (FIA) data from 11 Western conterminous States were used to (1) estimate and map the climatic profiles of tree species and (2) explore how to include climate variables in individual tree growth equations used in the Forest Vegetation Simulator (FVS). On the first front, we found the FIA data to be useful as training data in Breiman's...

Fresh Ideas, Perspectives, and Protocols Associated with Forest Inventory and Analysis Surveys: Graduate Reports, 1974 to July 2001

Treesearch

Victor A. Rudis

2003-01-01

Graduate M.S. theses and Ph.D. dissertations were searched to provide a body of information associated with the U.S. Department of Agriculture, Forest Service, Forest Inventory and Analysis (FIA) database. Authors' abstracts were included if available in electronic form and published since 1974. Novel technical and nontraditional FIA data uses, as well as the...

Monitoring Across Borders: 2010 Joint Meeting of the Forest Inventory and Analysis (FIA) Symposium and the Southern Mensurationists

Treesearch

Will McWilliams; Francis A. Roesch

2012-01-01

These proceedings represent the range of topics covered during the 2010 Joint Meeting of the Forest Inventory and Analysis (FIA) Symposium and the Southern Mensurationists, October 5-7, 2010 in Knoxville, TN. The meeting was a gathering of forest scientists with a quantitative leaning and, as such, the papers discuss the aspects of the observation, estimation, modeling...

Integrating forest inventory and analysis data into a LIDAR-based carbon monitoring system

Treesearch

Kristofer D. Johnson; Richard Birdsey; Andrew O Finley; Anu Swantaran; Ralph Dubayah; Craig Wayson; Rachel Riemann

2014-01-01

Forest Inventory and Analysis (FIA) data may be a valuable component of a LIDAR-based carbon monitoring system, but integration of the two observation systems is not without challenges. To explore integration methods, two wall-to-wall LIDAR-derived biomass maps were compared to FIA data at both the plot and county levels in Anne Arundel and Howard Counties in Maryland...

Forest Inventory and Analysis (FIA) annual inventory answers the question: What is happening to pinyon-juniper woodlands?

Treesearch

John D. Shaw; Brytten E. Steed; Larry T. DeBlander

2005-01-01

Widespread mortality in the pinyon-juniper forest type is associated with several years of drought in the southwestern United States. A complex of drought, insects, and disease is responsible for pinyon mortality rates approaching 100% in some areas, while other areas have experienced little or no mortality. Implementation of the Forest Inventory and Analysis (FIA)...

Farmers and woods: a look at woodlands and woodland-owner intentions in the heartland

Treesearch

W. Keith Moser; Earl C. Leatherberry; Mark H. Hansen; Brett Butler

2005-01-01

This paper reports the results of a pilot study that explores the relationship between farm woodland owners` stated intentions for owning woodland, and their use of the land, with the structure and composition of the woodland. Two databases maintained by the USDA Forest Service, Forest Inventory and Analysis (FIA) program were used in the analysis-- the FIA forest...

Leveraging FIA data for analysis beyond forest reports: examples from the world of carbon

Treesearch

Brian F. Walters; Grant M. Domke; Christopher W. Woodall

2015-01-01

The Forest Inventory and Analysis program of the USDA Forest Service is the go-to source for data to estimate carbon stocks and stock changes for the annual national greenhouse gas inventory (NGHGI) of the United States. However, the different pools of forest carbon have not always been estimated directly from FIA measurements. As part of the new forest carbon...

New Mexico Forest Inventory and Analysis: American Recovery and Reinvestment Act Project, Field Report: 2010-2012

Treesearch

Mary Stuever; John Capuano

2014-01-01

For a 3-year period, from 2010-2012, the New Mexico Forestry Division utilized contractors to collect Forest Inventory and Analysis (FIA) data in New Mexico. Funded through the American Recovery and Reinvestment Act, the State partnered with the Interior West FIA Program. Together, both agencies collected data on approximately 6,450 plots. This effort represents the...

The enhanced forest inventory and analysis program of the USDA forest service: historical perspective and announcements of statistical documentation

Treesearch

Ronald E. McRoberts; William A. Bechtold; Paul L. Patterson; Charles T. Scott; Gregory A. Reams

2005-01-01

The Forest Inventory and Analysis (FIA) program of the USDA Forest Service has initiated a transition from regional, periodic inventories to an enhanced national FIA program featuring annual measurement of a proportion of plots in each state, greater national consistency, and integration with the ground sampling component of the Forest Health Monitoring (FHM) program...

Allometric scaling theory applied to FIA biomass estimation

Treesearch

David C. Chojnacky

2002-01-01

Tree biomass estimates in the Forest Inventory and Analysis (FIA) database are derived from numerous methodologies whose abundance and complexity raise questions about consistent results throughout the U.S. A new model based on allometric scaling theory ("WBE") offers simplified methodology and a theoretically sound basis for improving the reliability and...

Multiple value forest surveys in the Midsouth states

Treesearch

Victor A. Rudis

1990-01-01

State-of-the-art achievement and limitations in integrating water, range, wildlife, and recreation ("nontimber") inventories with forest surveys of the USDA-Forest Service, Southern Forest Experiment station, Forest Inventory and Analysis (FIA) Unit are reviewed.The FIA Unit surveys private and public forests in 7 Midsouth states:Alabama, Arkansas, Louisiana...

FIAMODEL: Users Guide Version 3.0.

Treesearch

Scott A. Pugh; David D. Reed; Kurt S. Pregitzer; Patrick D. Miles

2002-01-01

FIAMODEL is a geographic information system (GIS program used to summarize Forest Inventory and Analysis (FIA, USDA Forest Service) data such as volume. The model runs in ArcView and allows users to select FIA plots with heads-up-digitizing, overlays of digital map layers, or queries based on specific plot attributes.

Forest land area estimates from vegetation continuous fields

Treesearch

Mark D. Nelson; Ronald E. McRoberts; Matthew C. Hansen

2004-01-01

The USDA Forest Service's Forest Inventory and Analysis (FIA) program provides data, information, and knowledge about our Nation's forest resources. FIA regional units collect data from field plots and remotely sensed imagery to produce statistical estimates of forest extent (area); volume, growth, and removals; and health and condition. There is increasing...

Advancements in LiDAR-based registration of FIA field plots

Treesearch

Demetrios Gatziolis

2012-01-01

Meaningful integration of National Forest Inventory field plot information with spectral imagery acquired from satellite or airborne platforms requires precise plot registration. Global positioning system-based plot registration procedures, such as the one employed by the Forest Inventory and Analysis (FIA) Program, yield plot coordinates that, although adequate for...

Antibody response in sheep following immunization with Streptococcus bovis in different adjuvants.

PubMed

Shu, Q; Bir, S H; Gill, H S; Duan, E; Xu, Y; Hiliard; Rowe, J B

2001-01-01

Recent studies have shown that immunization with Streptococcus bovis using Freund's complete adjuvant (FCA) may confer protection against lactic acidosis in sheep. The major objective of this study was to compare the antibody responses to S. bovis in a practically acceptable adjuvant (Freund's incomplete adjuvant (FIA); QuilA; dextran sulphate (Dex); Imject Alum; or Gerbu) and in FCA. Thirty-five sheep were randomly allocated to 7 treatment groups. Six groups were immunized with S. bovis in an adjuvant; the other group served as the non-immunization control. The primary immunization was administered intramuscularly on day 0. followed by a booster injection on day 28. Immunization with FCA induced the highest saliva and serum antibody responses. The saliva antibody concentrations in the FIA and QuilA groups were significantly higher than those in the Alum, Dex and Gerbu groups (p < 0.01). The serum antibody concentration in the FIA group was significantly higher than those in the QuilA, Alum. Dex and Gerbu groups (p < 0.01). Immunization enhanced the antibody level in faeces (p < 0.05), but there was no significant difference between the different adjuvant groups (p > 0.05). Seven and 14 days following booster immunization, the saliva antibody levels induced by QuilA and/or FIA were comparable with the level stimulated by FCA (p > 0.05). There was a strongly positive correlation (R2 = 0.770, p < 0.01) between the antibody concentrations in salival and serum. Compared with the controls, a higher faecal dry matter content was observed in the animals immunized with either FCA or QuilA. The change in faecal dry matter content was positively associated with the faecal antibody concentration (R2 = 0.441, p < 0.05). These results indicate that FIA and QuilA were effective at inducing high levels of antibody responses to S. bovis, and suggest that either Freund's incomplete adjuvant or QuilA may be useful for preparing a practically acceptable vaccine against lactic acidosis.

Measurement of forest disturbance and regrowth with Landsat and forest inventory and analysis data: anticipated benefits from forest and inventory analysis' collaboration with the national aeronautics and space administration and university partners

Treesearch

Sean Healey; Gretchen Moisen; Jeff Masek; Warren Cohen; Sam Goward; < i> et al< /i>

2007-01-01

The Forest Inventory and Analysis (FIA) program has partnered with researchers from the National Aeronautics and Space Administration, the University of Maryland, and other U.S. Department of Agriculture Forest Service units to identify disturbance patterns across the United States using FIA plot data and time series of Landsat satellite images. Spatially explicit...

Sensitive and selective determination of gallic acid in green tea samples based on an electrochemical platform of poly(melamine) film.

PubMed

Su, Ya-Ling; Cheng, Shu-Hua

2015-12-11

In this work, an electrochemical sensor coupled with an effective flow-injection amperometry (FIA) system is developed, targeting the determination of gallic acid (GA) in a mild neutral condition, in contrast to the existing electrochemical methods. The sensor is based on a thin electroactive poly(melamine) film immobilized on a pre-anodized screen-printed carbon electrode (SPCE*/PME). The characteristics of the sensing surface are well-characterized by field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS) and surface water contact angle experiments. The proposed assay exhibits a wide linear response to GA in both pH 3 and pH 7.0 phosphate buffer solutions (PBS) under the optimized flow-injection amperometry. The detection limit (S/N = 3) is 0.076 μM and 0.21 μM in the pH 3 and pH 7 solutions, respectively. A relative standard deviation (RSD) of 3.9% is obtained for 57 successive measurements of 50 μM GA in pH 7 solutions. Interference studies indicate that some inorganic salts, catechol, caffeine and ascorbic acid do not interfere with the GA assay. The interference effects from some orthodiphenolic compounds are also investigated. The proposed method and a conventional Folin-Ciocalteu method are applied to detect GA in green tea samples using the standard addition method, and satisfactory spiked recoveries are obtained. Copyright © 2015 Elsevier B.V. All rights reserved.

Feasibility of field portable near infrared (NIR) spectroscopy to determine cyanide concentrations in soil

NASA Astrophysics Data System (ADS)

Sut, Magdalena; Fischer, Thomas; Repmann, Frank; Raab, Thomas

2013-04-01

In Germany, at more than 1000 sites, soil is polluted with an anthropogenic contaminant in form of iron-cyanide complexes. These contaminations are caused by former Manufactured Gas Plants (MGPs), where electricity for lighting was produced in the process of coal gasification. The production of manufactured gas was restrained in 1950, which caused cessation of MGPs. Our study describes the application of Polychromix Handheld Field Portable Near-Infrared (NIR) Analyzer to predict the cyanide concentrations in soil. In recent times, when the soil remediation is of major importance, there is a need to develop rapid and non-destructive methods for contaminant determination in the field. In situ analysis enables determination of 'hot spots', is cheap and time saving in comparison to laboratory methods. This paper presents a novel usage of NIR spectroscopy, where a calibration model was developed, using multivariate calibration algorithms, in order to determine NIR spectral response to the cyanide concentration in soil samples. As a control, the contaminant concentration was determined using conventional Flow Injection Analysis (FIA). The experiments revealed that portable near-infrared spectrometers could be a reliable device for identification of contamination 'hot spots', where cyanide concentration are higher than 2400 mg kg-1 in the field and >1750 mg kg-1 after sample preparation in the laboratory, but cannot replace traditional laboratory analyses due to high limits of detection.

A tool to determine crown and plot canopy transparency for forest inventory and analysis phase 3 plots using digital photographs

Treesearch

Matthew F. Winn; Philip A. Araman

2012-01-01

The USDA Forest Service Forest Inventory and Analysis (FIA) program collects crown foliage transparency estimates for individual trees on Phase 3 (P3) inventory plots. The FIA crown foliage estimate is obtained from a pair of perpendicular side views of the tree. Researchers with the USDA Forest Service Southern Research Station have developed a computer program that...

Preparation of Forest Inventory and Analysis (FIA) and State Soil Geographic Data Base (STATSGO) data for global change research in the Eastern United States

Treesearch

Loius R. Iverson; Anantha M. G. Prasad; Charles T. Scott

1996-01-01

The USDA Forest Service's Forest Inventory and Analysis (FIA) and the Natural Resource Conservation Service's State Soil Geographic (STATSGO) data bases provide valuable natural resource data that can be analyzed at the national scale. When coupled with other data (e.g., climate), these data bases can provide insights into factors associated with current and...

Incorporation of Precipitation Data Into FIA Analyses: A Case Study of Factors Influencing Susceptibility to Oak Decline in Southern Missouri, U.S.A.

Treesearch

W. Keith Moser; Greg Liknes; Mark Hansen; Kevin Nimerfro

2005-01-01

The Forest Inventory and Analysis program at the North Central Research Station focuses on understanding the forested ecosystems in the North Central and Northern Great Plains States through analyzing the results of annual inventories. The program also researches techniques for data collection and analysis. The FIA process measures the above-ground vegetation and the...

Change detection for soil carbon in the forest inventory and analysis

Treesearch

An-Min Wu; Edward A. Nater; Charles H. Perry; Brent J. Dalzell; Barry T. Wilson

2015-01-01

Estimates of carbon stocks and stock changes in the U.S. Department of Agriculture Forest Service’s Forest Inventory and Analysis (FIA) Program are reported as the official United States submission to the UN Framework Convention on Climate Change. Soil, as a critical component of the forest carbon stocks, has been sampled in about 10-year intervals in FIA with the re-...

Feasibility of high-density climate reconstruction based on Forest Inventory and Analysis (FIA) collected tree-ring data

Treesearch

R. Justin DeRose; Shih-Yu Wang; John D. Shaw

2013-01-01

This study introduces a novel tree-ring dataset, with unparalleled spatial density, for use as a climate proxy. Ancillary Douglas fir and pinyon pine tree-ring data collected by the U.S. Forest Service Forest Inventory and Analysis Program (FIA data) were subjected to a series of tests to determine their feasibility as climate proxies. First, temporal coherence between...

Flow Injection Technique for Biochemical Analysis with Chemiluminescence Detection in Acidic Media

PubMed Central

Chen, Jing; Fang, Yanjun

2007-01-01

A review with 90 references is presented to show the development of acidic chemiluminescence methods for biochemical analysis by use of flow injection technique in the last 10 years. A brief discussion of both the chemiluminescence and flow injection technique is given. The proposed methods for biochemical analysis are described and compared according to the used chemiluminescence system.

Forests of Massachusetts, 2016

Treesearch

Brett J. Butler

2017-01-01

This report provides an overview of forest resources in Massachusetts based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station.Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2011-2016 with comparisons made to 2007-...

Forests of Massachusetts, 2013

Treesearch

Brett J. Butler

2014-01-01

This report provides an overview of forest resources in Massachusetts based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2009-2013 with comparisons made to 2003-...

Forests of North Dakota, 2013

Treesearch

David E. Haugen

2014-01-01

This resource update provides an overview of forest resources in North Dakota based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the North Dakota Forest Service. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Connecticut, 2013

Treesearch

Brett J. Butler

2014-01-01

This report provides an overview of forest resources in Connecticut based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2009-2013 with comparisons made to 2003-...

Forests of Michigan, 2013

Treesearch

Scott A. Pugh

2014-01-01

This publication provides an overview of forest resources in Michigan based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Since 1999, FIA has employed an annual inventory measuring data on a nominal 20 percent of sample plots each year. For the 2013 inventory, estimates for current...

Forests of Minnesota, 2013

Treesearch

Patrick D. Miles; Curtis VanderSchaaf

2014-01-01

This science update provides an overview of forest resources in Minnesota based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Minnesota Department of Natural Resources. Estimates are based on field data, collected using the FIA annualized sample design, for the...

Forests of Missouri, 2013

Treesearch

Ronald J. Piva; Thomas B. Treiman

2014-01-01

This science update provides an overview of forest resources in Missouri based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Missouri Department of Conservation. Estimates are based on field data, collected using the FIA annualized sample design, for the...

Forests of Iowa, 2013

Treesearch

Mark D. Nelson; Matt Brewer

2014-01-01

This resource update provides an overview of forest resources in Iowa based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Iowa Department of Natural Resources. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Vermont, 2013

Treesearch

Randall S. Morin; Scott A. Pugh

2014-01-01

This publication provides an overview of forest resources in Vermont based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Since 1999, FIA has employed an annual inventory measuring data on a nominal 20 percent of sample plots each year. For the 2013 inventory, estimates for current...

Forests of New Hampshire, 2013

Treesearch

Randall S. Morin; Scott A. Pugh

2014-01-01

This publication provides an overview of forest resources in New Hampshire based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Since 1999, FIA has employed an annual inventory measuring data on a nominal 20 percent of sample plots each year. For the 2013 inventory, estimates for...

Forests of Indiana, 2013

Treesearch

Dale D. Gormanson

2014-01-01

This resource update provides an overview of forest resources in Indiana based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Indiana Department of Natural Resources. Estimates are based on field data collected using the FIA annualized strategic sample design and...

Forests of North Carolina, 2013

Treesearch

Mark J. Brown

2015-01-01

This periodic resource update provides an overview of forest resources in North Carolina based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the North Carolina Forest Service. Data estimates are based on field data collected using the FIA annualized sample design...

Forests of Michigan, 2014

Treesearch

Scott A. Pugh

2015-01-01

This resource update provides an overview of forest resources in Michigan based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly.* The annual inventory started in 1999. For the 2014...

Forests of Connecticut, 2015

Treesearch

Brett J. Butler

2016-01-01

This report provides an overview of forest resources in Connecticut based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2010-2015 with comparisons made to 2005-...

Tree crown conditions in Missouri, 2000-2003

Treesearch

KaDonna C. Randolph; W. Keith Moser

2009-01-01

The Forest Service, U.S. Department of Agriculture, Forest Inventory and Analysis (FIA) Program uses visual assessments of tree crown condition to monitor changes and trends in forest health. This report describes three FIA tree crown condition indicators (crown dieback, crown density, and foliage transparency) and sapling crown vigor measured in Missouri between 2000...

Forests of Oklahoma, 2013

Treesearch

S. Lambert; J.T. Vogt.; J. Cooper

2015-01-01

This resource update provides an overview of forest resources in Oklahoma based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station, in cooperation with Oklahoma Forestry Services (OFS). Estimates are based on field data collected using the FIA annualized sample design and are updated yearly...

Kansas' forest resources, 2005

Treesearch

W. Keith Moser; Gary J. Brand; Melissa Powers

2007-01-01

The USDA Forest Service, Northern Research Station, Forest Inventory and Analysis (NRS-FIA) program is changing to a Web-based, dynamically linked reporting system. As part of the process, this year NRS-FIA is producing this abbreviated summary of 2005 data. This resource bulletin reports on area, volume, and biomass using data from 2001 through 2005. Estimates from...

Forests of Iowa, 2017

Treesearch

Mark D. Nelson; Tivon E. Feeley

2018-01-01

This resource update provides an overview of forest resources in Iowa based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Iowa Department of Natural Resources. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Georgia, 2013

Treesearch

T.J. Brandeis

2015-01-01

This resource update provides an overview of forest resources in Georgia based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Georgia Forestry Commission. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly....

Forests of Missouri, 2015

Treesearch

Ronald J. Piva; Thomas B. Treiman

2016-01-01

This resource update provides an overview of forest resources in Missouri based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Missouri Department of Conservation. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Pennsylvania, 2015

Treesearch

Richard H. Widmann

2016-01-01

This resource update provides an overview of the forest resources in Pennsylvania based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station (NRS). Estimates are based on field data collected using the FIA annualized sample design and are updated yearly1(see footnote 1, page 2). Information...

Forests of Michigan, 2015

Treesearch

Scott A. Pugh; Charles Paulson; Brett J. Butler

2016-01-01

This resource update provides an overview of forest resources in Michigan based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. The annual inventory started in 1999. For the 2015...

Forests of Ohio, 2015

Treesearch

Richard H. Widmann

2016-01-01

This resource update provides an overview of the forest resources in Ohio based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly.1(See footnotes on page 4.) Information about the...

Forests of Michigan, 2016

Treesearch

Charles Paulson; Scott A. Pugh

2017-01-01

This resource update provides an overview of forest resources in Michigan based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. The annual inventory started in 1999. For the 2016...

Forests of Massachusetts, 2015

Treesearch

Brett J. Butler

2016-01-01

This report provides an overview of forest resources in Massachusetts based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station.Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2010-2015 with comparisons made to 2005-...

Statistical properties of alternative national forest inventory area estimators

Treesearch

Francis Roesch; John Coulston; Andrew D. Hill

2012-01-01

The statistical properties of potential estimators of forest area for the USDA Forest Service's Forest Inventory and Analysis (FIA) program are presented and discussed. The current FIA area estimator is compared and contrasted with a weighted mean estimator and an estimator based on the Polya posterior, in the presence of nonresponse. Estimator optimality is...

Forest of North Dakota, 2016

Treesearch

David E. Haugen

2017-01-01

This resource update provides an overview of forest resources in North Dakota based on an inventory conducted by the USDA Forest Service, Forest Inventory and Analysis (FIA) program within the Northern Research Station in cooperation with the North Dakota Forest Service. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of North Carolina, 2014

Treesearch

Mark Brown; Samuel Lambert

2016-01-01

This periodic resource update provides an overview of forest resources in North Carolina based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the North Carolina Forest Service. Data estimates are based on field data collected using the FIA annualized sample design...

Forests of North Dakota, 2017

Treesearch

Charles S. Paulson

2018-01-01

This resource update provides an overview of forest resources in North Dakota based on an inventory conducted by the USDA Forest Service, Forest Inventory and Analysis (FIA) program within the Northern Research Station in cooperation with the North Dakota Forest Service. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Pennsylvania, 2016

Treesearch

Thomas A. Albright

2017-01-01

This resource update provides an overview of the forest resources in Pennsylvania based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. Information about the national and regional...

Forests of New Jersey, 2015

Treesearch

Susan J. Crocker; Brett J. Butler

2016-01-01

This publication provides an overview of forest resources in New Jersey based on an annual inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station. Estimates are based on field data collected using an annualized sample design and are updated yearly. Information about the FIA program is available at...

Forests of Connecticut, 2016

Treesearch

Brett J. Butler

2017-01-01

This report provides an overview of forest resources in Connecticut based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2011-2016 with comparisons made to 2007-...

Forests of Missouri, 2017

Treesearch

Thomas C. Goff

2018-01-01

This resource update provides an overview of forest resources in Missouri based on an inventory conducted by the USDA Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Missouri Department of Conservation. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Michigan, 2017

Treesearch

Scott A. Pugh

2018-01-01

This resource update provides an overview of forest resources in Michigan based on inventories conducted by the USDA Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. The annual inventory started in 1999. For the 2017...

Forests of Missouri, 2016

Treesearch

Ronald J. Piva; Thomas B. Treiman

2017-01-01

This resource update provides an overview of forest resources in Missouri based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Missouri Department of Conservation. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of New Jersey, 2016

Treesearch

Susan J. Crocker; Greg C. Liknes

2017-01-01

This publication provides an overview of forest resources in New Jersey following an inventory by the U.S. Forest Service, Forest Inventory and Analysis program (FIA), Northern Research Station (NRS). Estimates are derived from field data collected using an annualized sample design and are updated yearly. Beginning in 2014, NRS-FIA switched to a 7-year cycle length....

Tiger 2000 and FIA

Treesearch

Joseph McCollum; Dennis Jacobs

2002-01-01

legal foundations of the FIA (Forest Inventory and Analysis) progrdm are laid out. Upon those foundations are built a geographical definition of the United States and its conlponents, and how applying that definition might change from decade to decade. Along the way, the American system of weights and measures as well as the unusual geography of the commonwealth of...

Forests of Iowa, 2015

Treesearch

Mark D. Nelson; Matt Brewer; Dacia M. Meneguzzo; Kathryne. Clark

2016-01-01

This resource update provides an overview of forest resources in Iowa based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Iowa Department of Natural Resources. Estimates are based on field data collected using the FIA annualized sample design and are updated...

An evaluation of FIA's stand age variable

Treesearch

John D. Shaw

2015-01-01

The Forest Inventory and Analysis Database (FIADB) includes a large number of measured and computed variables. The definitions of measured variables are usually well-documented in FIA field and database manuals. Some computed variables, such as live basal area of the condition, are equally straightforward. Other computed variables, such as individual tree volume,...

Precise FIA plot registration using field and dense LIDAR data

Treesearch

Demetrios Gatziolis

2009-01-01

Precise registration of forest inventory and analysis (FIA) plots is a prerequisite for an effective fusion of field data with ancillary spatial information, which is an approach commonly employed in the mapping of various forest parameters. Although the adoption of Global Positioning System technology has improved the precision of plot coordinates obtained during...

Forests of Alabama, 2015

Treesearch

Andy Hartsell

2016-01-01

This resource update provides an overview of forest resources in Alabama based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Alabama Forestry Commission. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly....

Forests of Indiana, 2015

Treesearch

Dale D. Gormanson

2016-01-01

This resource update provides an overview of forest resources in Indiana based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Indiana Department of Natural Resources. Estimates are based on field data collected using the FIA annualized strategic sample design and...

Forests of Indiana, 2016

Treesearch

Dale D. Gormanson; Cassandra M. Kurtz

2017-01-01

This resource update provides an overview of forest resources in Indiana based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Indiana Department of Natural Resources. Estimates are based on field data collected using the FIA annualized strategic sample design and...

Forests of Louisiana, 2014

Treesearch

S.N. Oswalt

2017-01-01

This resource update provides an overview of forest resources in Louisiana based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. The estimates presented in this update are for the...

Forests of Louisiana, 2012

Treesearch

S.N. Oswalt

2014-01-01

This resource update provides an overview of forest resources in Louisiana based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Mississippi Forestry Commission. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Iowa, 2014

Treesearch

Mark D. Nelson; Matt Brewer; Brett J. Butler; Scott A. Pugh

2015-01-01

This resource update provides an overview of forest resources in Iowa based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Iowa Department of Natural Resources. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Massachusetts, 2014

Treesearch

Brett J. Butler; Susan J. Crocker

2015-01-01

This report provides an overview of forest resources in Massachusetts based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2009-2014 with comparisons made to 2005-...

Forests of Pennsylvania, 2014

Treesearch

Richard H. Widmann

2015-01-01

This resource update provides an overview of the forest resources in Pennsylvania based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly (see footnote 1 on page 4). Information about...

Forests of Oklahoma, 2014

Treesearch

S. Lambert; K. Randolph; J. Cooper

2015-01-01

This resource update provides an overview of forest resources in Oklahoma based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station, in cooperation with Oklahoma Forestry Services. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly,...

Forests of Indiana, 2014

Treesearch

Dale D. Gormanson; Ronald J. Piva

2015-01-01

This resource update provides an overview of forest resources in Indiana based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Indiana Department of Natural Resources. Estimates are based on field data collected using the FIA annualized strategic sample design and...

Forests of Kentucky, 2014

Treesearch

Thomas Brandeis; Andy Hartsell; KaDonna Randolph; Sonja Oswalt; Consuelo Brandeis

2016-01-01

This resource update provides an overview of forest resources in Kentucky based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. The estimates presented in this update are...

Forests of Alabama, 2014

Treesearch

Andy Hartsell

2016-01-01

This resource update provides an overview of forest resources in Alabama based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Alabama Forestry Commission. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly....

Forests of Wisconsin, 2014

Treesearch

Charles H. Perry

2015-01-01

This resource update provides an overview of forest resources in Wisconsin based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Wisconsin Department of Natural Resources (WDNR). Data estimates are based on field data collected using the FIA annualized sample...

Forests of Ohio, 2014

Treesearch

Richard H. Widmann

2015-01-01

This resource update provides an overview of the forest resources in Ohio based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. (See footnote on page 4.) Information about the...

Forests of Connecticut, 2014

Treesearch

Brett J. Butler; Susan J. Crocker

2015-01-01

This report provides an overview of forest resources in Connecticut based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2009-2014 with comparisons made to 2005-...

Forests of North Dakota, 2014

Treesearch

D.E. Haugen; S.A. Pugh

2014-01-01

This resource update provides an overview of forest resources in North Dakota based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the North Dakota Forest Service. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Georgia, 2014

Treesearch

Thomas Brandeis; Andy Hartsell

2015-01-01

This resource update provides an overview of forest resources in Georgia based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Georgia Forestry Commission. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly....

Correct county areas with sidebars for Virginia

Treesearch

Joseph M. McCollum; Dale Gormanson; John Coulston

2009-01-01

Historically, Forest Inventory and Analysis (FIA) has processed field inventory data at the county level and county estimates of land area were constrained to equal those reported by the Census Bureau. Currently, the Southern Research Station FIA unit processes field inventory data at the survey unit level (groups of counties with similar ecological characteristics)....

Forests of Wisconsin, 2017

Treesearch

Cassandra M. Kurtz

2018-01-01

This resource update provides an overview of forest resources in Wisconsin based on an inventory conducted by the USDA Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Wisconsin Department of Natural Resources. Estimates are based on field data collected using the FIA annualized sample design and are...

Forests of Florida, 2012

Treesearch

M.J. Brown; Jarek. Nowak

2014-01-01

This periodic resource update provides an overview of forest resources in Florida based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Florida Forest Service. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Iowa, 2016

Treesearch

Mark D. Nelson; Tivon E. Feeley; Cassandra M. Kurtz

2017-01-01

This resource update provides an overview of forest resources in Iowa based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Iowa Department of Natural Resources. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Oklahoma, 2012

Treesearch

S. Lambert; J.A. Cooper

2014-01-01

This resource update provides an overview of forest resources in Oklahoma based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station, in cooperation with Oklahoma Forestry Services (OFS). Estimates are based on field data collected using the FIA annualized sample design and are updated yearly...

Refining FIA plot locations using LiDAR point clouds

Treesearch

Charlie Schrader-Patton; Greg C. Liknes; Demetrios Gatziolis; Brian M. Wing; Mark D. Nelson; Patrick D. Miles; Josh Bixby; Daniel G. Wendt; Dennis Kepler; Abbey Schaaf

2015-01-01

Forest Inventory and Analysis (FIA) plot location coordinate precision is often insufficient for use with high resolution remotely sensed data, thereby limiting the use of these plots for geospatial applications and reducing the validity of models that assume the locations are precise. A practical and efficient method is needed to improve coordinate precision. To...

FIA forest inventory data for wildlife habitat assessment

Treesearch

David C. Chojnacky

2000-01-01

The Forest Inventory and Analysis (FIA) program of the USDA Forest Service maintains a network of permanent plots to monitor changing forest conditions. These plots were originally established to monitor the nation's timber supply; however, these data have great potential for evaluating other forest resources. To demonstrate a wildlife application, an assessment...

Forests of Wisconsin, 2015

Treesearch

Charles H. Perry

2016-01-01

This Resource Update provides an overview of forest resources in Wisconsin based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Wisconsin Department of Natural Resources (WDNR). Data estimates are based on field data collected using the FIA annualized sample...

Anomalous dismeter distribution shifts estimated from FIA inventories through time

Treesearch

Francis A. Roesch; Paul C. Van Deusen

2010-01-01

In the past decade, the United States Department of Agriculture Forest Service’s Forest Inventory and Analysis Program (FIA) has replaced regionally autonomous, periodic, state-wide forest inventories using various probability proportional to tree size sampling designs with a nationally consistent annual forest inventory design utilizing systematically spaced clusters...

Sensitivity of FIA Volume Estimates to Changes in Stratum Weights and Number of Strata

Treesearch

James A. Westfall; Michael Hoppus

2005-01-01

In the Northeast region, the USDA Forest Service Forest Inventory and Analysis (FIA) program utilizes stratified sampling techniques to improve the precision of population estimates. Recently, interpretation of aerial photographs was replaced with classified remotely sensed imagery to determine stratum weights and plot stratum assignments. However, stratum weights...

A new Link for Geographic analyses of Inventory Data

Treesearch

David Reed; Kurt Pregitzer; Scott A. Pugh; Patrick D. Miles

2001-01-01

The USDA Forest Service Forest Inventory and Analysis (FIA)data are widely used throughout the United States for analyses of forest status and trends, landscape-level forest composition, and other forest characteristics. A new software product, FIAMODEL, is available for analyzing FIA data within the ArcView? (ESRI, Inc.)geographic information system. The software...

Integrating Spatial Components into FIA Models of Forest Resources: Some Technical Aspects

Treesearch

Pat Terletzky; Tracey Frescino

2005-01-01

We examined two software packages to determine their feasibility of implementing spatially explicit, forest resource models that integrate Forest Inventory and Analysis data (FIA). ARCINFO and Interactive Data Language (IDL) were examined for their input requirements, speed of processing, storage requirements, and flexibility of implementing. Implementations of two...

Forests of Florida, 2013

Treesearch

Mark Brown; J.. Nowak

2016-01-01

This periodic resource update provides an overview of forest resources in Florida based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Florida Forest Service. Estimates are based on field data collected using the FIA annualized sample design and are updated...

Forests of Alabama, 2016

Treesearch

A. Hartsell

2017-01-01

This resource update provides an overview of forest resources in Alabama based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Alabama Forestry Commission. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly....

Forests of Wisconsin, 2013

Treesearch

Charles H. Perry

2014-01-01

This resource update provides an overview of forest resources in Wisconsin based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Northern Research Station in cooperation with the Wisconsin Department of Natural Resources. Data estimates are based on field data collected using the FIA annualized sample design and...

Development of a Highly Specific Fluorescence Immunoassay for Detection of Diisobutyl Phthalate in Edible Oil Samples.

PubMed

Cui, Xiping; Wu, Panpan; Lai, Dan; Zheng, Shengwu; Chen, Yingshan; Eremin, Sergei A; Peng, Wei; Zhao, Suqing

2015-10-28

The diisobutyl phthalate (DiBP) hapten containing an amino group was synthesized successfully, and the polyclonal antibody against 4-amino phthalate-bovine serum albumin (BSA) was developed. On the basis of the polyclonal antibody, a rapid and sensitive indirect competitive fluorescence immunoassay (icFIA) has been established to detect DiBP in edible oil samples for the first time. Under the optimized conditions, the quantitative working range of the icFIA was from 10.47 to 357.06 ng/mL (R(2) = 0.991), exhibiting a detection limit of 5.82 ng/mL. In this assay, the specific results showed that other similar phthalates did not significantly interfere with the analysis, with the cross-reactivity less than 1.5%, except for that of DiBAP. Thereafter, DiBP contamination in edible oil samples was detected by icFIA, with the recovery being from 79 to 103%. Furthermore, the reliability of icFIA was validated by gas chromatography-mass spectrometry (GC-MS). Therefore, the developed icFIA is suitable for monitoring DiBP in some edible oil samples.

A report on the potential use of USDA Forest Service forest inventory and analysis data by the Bureau of Land Management

Treesearch

Bill Williams; Tracey S. Frescino; Larry T. DeBlander; Sharon W. Woudenberg; Michael Wilson

2006-01-01

The Bureau of Land Management (BLM) does not have a consistent internal program or source of vegetation data to use for strategic level planning, such as in resource management plans. This technical note discusses and evaluates the potential of the USDA Forest Service Forest Inventory and Analysis (FIA) Program to assist the BLM in filling this data gap. The FIA...

Assessing the impact of a mountain pine beetle infestation on stand structure of lodgepole pine forests in Colorado using the Forest Inventory and Analysis Annual forest inventory

Treesearch

Michael T. Thompson

2017-01-01

The Forest Inventory and Analysis (FIA) annual inventory system began in Colorado in 2002, which coincided with the onset of a major mountain pine beetle (Dendroctonus ponderosae) epidemic. The mortality event, coupled with 11 years of annual inventory data, provided an opportunity to assess the usefulness of the FIA annual inventory system for quantifying the effects...

Forest inventory and analysis program in the Western U.S.

Treesearch

Ashley Lehman

2015-01-01

The Pacific Northwest (PNW) Research Station’s Forest Inventory and Analysis (FIA) program of the USDA Forest Service monitors and reports on the status and trends of the Pacific Island’s forest resources and ecosystem services. Since 2001 the FIA program has partnered with State and Private Forestry’s, Region 5 and the local governments in the U.S. Affiliated Western...

Forest inventory and analysis program in the Western U.S

Treesearch

Ashley Lehman

2015-01-01

The Pacific Northwest (PNW) Research Station’s Forest Inventory and Analysis (FIA) program of the USDA Forest Service monitors and reports on the status and trends of the Pacific Island’s forest resources and ecosystem services. Since 2001 the FIA program has partnered with State and Private Forestry’s, Region 5 and the local governments in the U.S. Affiliated Western...

Progress in adapting k-NN methods for forest mapping and estimation using the new annual Forest Inventory and Analysis data

Treesearch

Reija Haapanen; Kimmo Lehtinen; Jukka Miettinen; Marvin E. Bauer; Alan R. Ek

2002-01-01

The k-nearest neighbor (k-NN) method has been undergoing development and testing for applications with USDA Forest Service Forest Inventory and Analysis (FIA) data in Minnesota since 1997. Research began using the 1987-1990 FIA inventory of the state, the then standard 10-point cluster plots, and Landsat TM imagery. In the past year, research has moved to examine...

Dissolution testing and potentiometric determination of famciclovir in pure, dosage forms and biological fluids.

PubMed

Rezk, Mohamed S; El Nashar, Rasha M

2013-02-01

The performance characteristics of two new plastic membrane ion selective electrodes (ISEs) used for the determination of famciclovir (Fcv) based on the ion associate of Fcv with phosphotungstic acid (PTA) or phosphomolybdic acid (PMA) are described. Different experimental conditions as type of plasticizer to be incorporated in the membrane, life span, effect of soaking, pH, temperature, and interferences were studied. Both electrodes showed similar performance under these conditions, exhibiting Nernstian slopes of S (Fcv-PTA)=58.60±0.84 mV/decade and S (Fcv-PMA)=58.77±0.68 mV/decade within a usable concentration range of 10⁻⁵-10⁻² [Fcv/M] at 298/K. Famciclovir was assayed potentiometrically in its pure solution, pharmaceutical preparations and biological fluids (urine and plasma) using proposed electrodes under batch and flow injection analysis (FIA) conditions with a recovery % ranging between 96.76% and 102.83% having RSD of 0.66%-1.81%. The electrodes were also successfully applied in the determination of the dissolution profile of Fcv tablets and the results came in agreement with the validated results of the HPLC method obtained from the quality control unit of the company producing the tablets. Copyright © 2012 Elsevier B.V. All rights reserved.

Location uncertainty and the tri-areal design

Treesearch

Francis A. Roesch

2007-01-01

The U.S. Department of Agriculture Forest Service Forest Inventory and Analysis Program (FIA) uses a field plot design that incorporates multiple sample selection mechanisms. Not all of the five FIA units currently use the entire suite of available sample selection mechanisms. These sampling selection mechanisms could be described in a number of ways with respect to...

Forests of Rhode Island, 2013

Treesearch

Brett J. Butler

2014-01-01

This report provides an overview of forest resources in Rhode Island based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2009-2013 with comparisons made to 2003-...

Forests of east Texas, 2013

Treesearch

K.J.W. Dooley; T.J. Brandeis

2014-01-01

This resource update provides an overview of forest resources in east Texas based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station in cooperation with the Texas A&M Forest Service. Forest resource estimates are based on field data collected using the FIA annualized sample design and...

Estimating mangrove in Florida: trials monitoring rare ecosystems

Treesearch

Mark J. Brown

2015-01-01

Mangrove species are keystone components in coastal ecosystems and are the interface between forest land and sea. Yet, estimates of their area have varied widely. Forest Inventory and Analysis (FIA) data from ground-based sample plots provide one estimate of the resource. Initial FIA estimates of the mangrove resource in Florida varied dramatically from those compiled...

Effect of inventory method on niche models: random versus systematic error

Treesearch

Heather E. Lintz; Andrew N. Gray; Bruce McCune

2013-01-01

Data from large-scale biological inventories are essential for understanding and managing Earth's ecosystems. The Forest Inventory and Analysis Program (FIA) of the U.S. Forest Service is the largest biological inventory in North America; however, the FIA inventory recently changed from an amalgam of different approaches to a nationally-standardized approach in...

Adding net growth, removals, and mortality estimates for biomass and carbon in FIADB

Treesearch

Jeffery A. Turner

2015-01-01

Traditional growth, removals, and mortality (GRM) estimates produced from Forest Inventory and Analysis (FIA) periodic inventories were limited to changes in volume on timberland. Estimates on forestland were added in the east as the first installment of annual inventory plots was remeasured. The western FIA units have begun annual remeasurement, precipitating the need...

Forests of Delaware, 2015

Treesearch

Tonya Lister; Richard Widmann

2016-01-01

This publication provides an overview of forest resources in Delaware based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. From 2004-2013, FIA employed an annual inventory with a cycle length of 5 years, measuring data on 20 percent of all sample plots each year in Delaware. Beginning...

Forests of New York, 2015

Treesearch

Richard H. Widmann

2016-01-01

This resource update provides an overview of the forest resources in New York based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly.1(See footnote on page 4). Information about the...

Forests of Rhode Island, 2015

Treesearch

Brett J. Butler

2016-01-01

This report provides an overview of forest resources in Rhode Island based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2010-2015 with comparisons made to 2005-...

Forests of Rhode Island, 2016

Treesearch

Brett J. Butler

2017-01-01

This report provides an overview of forest resources in Rhode Island based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2011-2016 with comparisons made to 2007-...

Forests of Delaware, 2016

Treesearch

Stephen Potter

2017-01-01

This publication provides an overview of forest resources in Delaware based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. From 2004-2013, FIA employed an annual inventory with a cycle length of 5 years, measuring data on 20 percent of all sample plots each year in Delaware. Beginning...

TIGER 2000 and FIA

Treesearch

Joseph McCollum; Dennis Jacobs

2005-01-01

The legal foundations of the FIA (Forest Inventory and Analysis) program are laid out. Upon those foundations are built a geographical definition of the United States and its components, and how applying that definition might change from decade to decade. Along the way, the American system of weights and measures as well as the unusual geography of the Commonwealth of...

Comparing Forest/Nonforest Classifications of Landsat TM Imagery for Stratifying FIA Estimates of Forest Land Area

Treesearch

Mark D. Nelson; Ronald E. McRoberts; Greg C. Liknes; Geoffrey R. Holden

2005-01-01

Landsat Thematic Mapper (TM) satellite imagery and Forest Inventory and Analysis (FIA) plot data were used to construct forest/nonforest maps of Mapping Zone 41, National Land Cover Dataset 2000 (NLCD 2000). Stratification approaches resulting from Maximum Likelihood, Fuzzy Convolution, Logistic Regression, and k-Nearest Neighbors classification/prediction methods were...

Forests of east Texas, 2016

Treesearch

Kerry Dooley

2018-01-01

This resource update provides an overview of forest resources in east Texas based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station (SRS) in cooperation with Texas A&M Forest Service. The 254 counties of Texas are consolidated into seven FIA survey units—Southeast (unit 1),...

A discrete global grid of photointerpretation

Treesearch

Joseph M McCollum; Jamie K. Cochran; Anita K. Rose

2008-01-01

The Forest Inventory and Analysis (FIA) Program of the Forest Service, U.S.Department of Agriculture, collects its data in three phases. The first phase is collection of photointerpretation data or dot counts, the second phase is field collection of FIA plot data, and the third phase is collection of Forest Health Monitoring data. This paper describes the development...

Alabama, 2010 forest inventory and analysis factsheet

Treesearch

Andrew J. Hartsell

2011-01-01

FIA was initially established to monitor the Nation’s timber supply and the amount of commercially available resources. These early surveys were not concerned with the forests, species, and tree sizes that were not considered commercially viable. Early FIA reported only on growing-stock trees on timberlands, i.e., commercially important tree species and sizes on...

Forests of New York, 2014

Treesearch

Richard H. Widmann

2015-01-01

This resource update provides an overview of the forest resources in New York based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design and are updated yearly. (See footnote on page 4). Information about the...

Forests of Delaware, 2014

Treesearch

T.W. Lister; R.H. Widmann

2015-01-01

This publication provides an overview of forest resources in Delaware based on inventories conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. From 2004-2013, FIA employed an annual inventory with a cycle length of 5 years, measuring data on 20 percent of all sample plots each year in Delaware. Beginning...

Forests of Rhode Island, 2014

Treesearch

Brett J. Butler; Susan J. Crocker

2015-01-01

This report provides an overview of forest resources in Rhode Island based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program of the Northern Research Station. Estimates are based on field data collected using the FIA annualized sample design. Results are for the measurement years 2009-2014 with comparisons made to 2005-...

Forests of Illinois, 2014

Treesearch

Susan J. Crocker

2015-01-01

This publication provides an overview of forest resource attributes for Illinois based on an annual inventory conducted by the Forest Inventory and Analysis (FIA) program of the Northern Research Station (NRS) of the U.S. Forest Service. These estimates, along with web-posted core tables, are updated annually. In 2014, NRS-FIA changed from a 5- to a 7-year inventory...

Forests of New Jersey, 2014

Treesearch

Susan J. Crocker

2015-01-01

This publication provides an overview of forest resource attributes for New Jersey based on an annual inventory conducted by the Forest Inventory and Analysis (FIA) program of the Northern Research Station (NRS) of the U.S. Forest Service. These estimates, along with web-posted core tables, are updated annually. In 2014, NRS-FIA changed from a 5- to a 7-year inventory...

Landscape scale mapping of forest inventory data by nearest neighbor classification

Treesearch

Andrew Lister

2009-01-01

One of the goals of the Forest Service, U.S. Department of Agriculture's Forest Inventory and Analysis (FIA) program is large-area mapping. FIA scientists have tried many methods in the past, including geostatistical methods, linear modeling, nonlinear modeling, and simple choropleth and dot maps. Mapping methods that require individual model-based maps to be...

Estimating down deadwood from FIA forest inventory variables in Maine

Treesearch

David C. Chojnacky; Linda S. Heath

2002-01-01

Down deadwood (DDW) is a carbon component important in the function and structure of forest ecosystems, but estimating DDW is problematic because these data are not widely available in forest inventory databases. However, DDW data were collected on USDA Forest Service Forest Inventory and Analysis (FIA) plots during Maine's 1995 inventory. This study examines ways...

Estimating down dead wood from FIA forest inventory variables in Maine

Treesearch

David C. Chojnacky; Linda S. Heath

2002-01-01

Down deadwood (DDW) is a carbon component important in the function and structure of forest ecosystems, but estimating DDW is problematic because these data are not widely available in forest inventory databases. However, DDW data were collected on USDA Forest Service Forest Inventory and Analysis (FIA) plots during Maine's 1995 inventory. This study examines ways...

Vision for the Future of FIA: Paean to Progress, Possibilities, and Partners

Treesearch

Susan L. King; Charles T. Scott

2006-01-01

The Forest Inventory and Analysis (FIA) program of the U.S. Department of Agriculture Forest Service has made significant progress implementing the annualized inventory in 46 States in 2004. Major increases in program performance included the availability of plot data and the plots? corresponding approximate coordinates. A mill site study and biomass models were used...

What does nonforest land contribute to the global carbon balance?

Treesearch

Jennifer C. Jenkins; Rachel Riemann

2002-01-01

An inventory of land traditionally called "nonforest" and therefore not sampled by the Forest Inventory and Analysis (FIA) program was implemented by the FIA unit at the Northeastern Station in 1999 for five counties in Maryland. Biomass and biomass increment were estimated from the nonforest inventory data using techniques developed for application to large-...

Forests of East Texas, 2014

Treesearch

Thomas J. Brandeis

2015-01-01

This resource update provides an overview of forest resources in east Texas derived from an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) Program at the Southern Research Station in cooperation with the Texas A&M Forest Service. These estimates are based on field data collected using the FIA annualized sample design and are...

Fire effects assessment using FIA data in the northern and central Rocky Mountains

Treesearch

Theresa B. Jain; Ralph Their; Wilson Michael

2003-01-01

Wildfires of 2000 and 2001 burned thousands of hectares in the Northern Rocky Mountains. Within the fire parameters, 162 Forest Inventory and Analysis (FIA) plots burned in Idaho and Montana where pre-wildfire information on forest structure, vegetation composition, soil productivity, and surface fuels was documented; thus providing a unique opportunity to assess...

Broad-Scale Assessment of Fuel Treatment Opportunities

Treesearch

Patrick D. Miles; Kenneth E. Skog; Wayne D. Shepperd; Elizabeth D. Reinhardt; Roger D. Fight

2006-01-01

The Forest Inventory and Analysis (FIA) program has produced estimates of the extent and composition of the Nation?s forests for several decades. FIA data have been used with a flexible silvicultural thinning option, a fire hazard model for preharvest and postharvest fire hazard assessment, a harvest economics model, and geospatial data to produce a Web-based tool to...

Evaluating Classified MODIS Satellite Imagery as a Stratification Tool

Treesearch

Greg C. Liknes; Mark D. Nelson; Ronald E. McRoberts

2004-01-01

The Forest Inventory and Analysis (FIA) program of the USDA Forest Service collects forest attribute data on permanent plots arranged on a hexagonal network across all 50 states and Puerto Rico. Due to budget constraints, sample sizes sufficient to satisfy national FIA precision standards are seldom achieved for most inventory variables unless the estimation process is...

Lava, VOG, and tropical forests: working with the FIA program in Hawaii

Treesearch

Thomas McGinley; Ashley Lehman

2015-01-01

In the winter of 2009, the Pacific Northwest Research Station initiated the ground implementation of their Forest Inventory and Analysis (FIA) program on the Hawaiian Islands. In the Pacific, people from the indigenous to the transplanted, hold intrinsic and utilitarian values of their forests that often differ considerably from values of mainstream mainland USA. These...

FIAMODEL : a new link for geographic analyses of inventory data

Treesearch

David Reed; Kurt Pregitzer; Scott A. Pugh; Patrick Miles

2001-01-01

The USDA Forest Service Forest Inventory and Analysis (FIA) data are widely used throughtout the United States for analyses of forest status and trends, landscape-level forest composition, and other forest characteristics. A new software product, FIAMODEL, is available for analyzing FIA data within the ArcView (ESRI,Inc.) geographic information system. The software...

Forest Resources of Isle Royale National Park 2010

Treesearch

Wilfred J. Previant; Linda M. Nagel; Scott A. Pugh; Christopher W. Woodall

2012-01-01

This publication provides a baseline overview of forest resources for Isle Royale National Park (Isle Royale) using data from the Forest Inventory and Analysis (FIA) Program of the U.S. Department of Agriculture, Forest Service. The availability of permanent FIA plots allows for the first-ever comparison of Isle Royale's forest conditions (2006-2010) to reserved...

Fragmentation statistics for FIA: designing an approach

Treesearch

Rachel Riemann; Andrew Lister; Michael Hoppus; Tonya Lister

2002-01-01

The USDA Forest Inventory and Analysis (FIA) program collects data on the amount of forest, as well as on characteristics such as forest type, tree volume, species composition, and size and age classes. However, little data are obtained nationwide on forest fragmentation-how that forest is distributed and in what land use/land cover context-factors that can...

Forests of east Texas, 2015

Treesearch

Kerry J.W. Dooley

2017-01-01

This resource update provides an overview of forest resources in east Texas based on an inventory conducted by the U.S. Forest Service, Forest Inventory and Analysis (FIA) program at the Southern Research Station (SRS) in cooperation with Texas A&M Forest Service. The 254 counties of Texas are consolidated into seven FIA survey units—Southeast (unit 1),...

Hardwood Projections For Southeastern U.S.

Treesearch

William Bechtold

1988-01-01

Much of what is covered here is based on data collected by the Forest Inventory and Analysis (FIA) Work Unit of the Forest Service Southeastern Forest Experiment Station. Southeast FIA is responsible of monitoring the forest resources of Florida, Georgia, North Carolina, South Carolina, and Virginia. The first survey of the Southeast began in Florida in 1934. Our field...

Bridging the gap between data analysis and data collection in FIA and forest monitoring globally: successes, research findings, and lessons learned from the Western US and Southeast Asia

Treesearch

Leif Mortenson

2015-01-01

Globally, national forest inventories (NFI) require a large work force typically consisting of multiple teams spread across multiple locations in order to successfully capture a given nation’s forest resources. This is true of the Forest Inventory and Analysis (FIA) program in the US and in many inventories in developing countries that are supported by USFS...

The combination of four analytical methods to explore skeletal muscle metabolomics: Better coverage of metabolic pathways or a marketing argument?

PubMed

Bruno, C; Patin, F; Bocca, C; Nadal-Desbarats, L; Bonnier, F; Reynier, P; Emond, P; Vourc'h, P; Joseph-Delafont, K; Corcia, P; Andres, C R; Blasco, H

2018-01-30

Metabolomics is an emerging science based on diverse high throughput methods that are rapidly evolving to improve metabolic coverage of biological fluids and tissues. Technical progress has led researchers to combine several analytical methods without reporting the impact on metabolic coverage of such a strategy. The objective of our study was to develop and validate several analytical techniques (mass spectrometry coupled to gas or liquid chromatography and nuclear magnetic resonance) for the metabolomic analysis of small muscle samples and evaluate the impact of combining methods for more exhaustive metabolite covering. We evaluated the muscle metabolome from the same pool of mouse muscle samples after 2 metabolite extraction protocols. Four analytical methods were used: targeted flow injection analysis coupled with mass spectrometry (FIA-MS/MS), gas chromatography coupled with mass spectrometry (GC-MS), liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS), and nuclear magnetic resonance (NMR) analysis. We evaluated the global variability of each compound i.e., analytical (from quality controls) and extraction variability (from muscle extracts). We determined the best extraction method and we reported the common and distinct metabolites identified based on the number and identity of the compounds detected with low analytical variability (variation coefficient<30%) for each method. Finally, we assessed the coverage of muscle metabolic pathways obtained. Methanol/chloroform/water and water/methanol were the best extraction solvent for muscle metabolome analysis by NMR and MS, respectively. We identified 38 metabolites by nuclear magnetic resonance, 37 by FIA-MS/MS, 18 by GC-MS, and 80 by LC-HRMS. The combination led us to identify a total of 132 metabolites with low variability partitioned into 58 metabolic pathways, such as amino acid, nitrogen, purine, and pyrimidine metabolism, and the citric acid cycle. This combination also showed that the contribution of GC-MS was low when used in combination with other mass spectrometry methods and nuclear magnetic resonance to explore muscle samples. This study reports the validation of several analytical methods, based on nuclear magnetic resonance and several mass spectrometry methods, to explore the muscle metabolome from a small amount of tissue, comparable to that obtained during a clinical trial. The combination of several techniques may be relevant for the exploration of muscle metabolism, with acceptable analytical variability and overlap between methods However, the difficult and time-consuming data pre-processing, processing, and statistical analysis steps do not justify systematically combining analytical methods. Copyright © 2017 Elsevier B.V. All rights reserved.

The poor man's Geographic Information System: plot expansion factors

Treesearch

Paul C. Van Deusen

2007-01-01

Plot expansion factors can serve as a crude Geographic Information System for users of Forest Inventory and Analysis (FIA) data. Each FIA plot has an associated expansion factor that is often interpreted as the number of forested acres that the plot represents. The derivation of expansion factors is discussed and it is shown that the mapped plot design requires a...

Relating FIA data to habitat classifications via tree-based models of canopy cover

Treesearch

Mark D. Nelson; Brian G. Tavernia; Chris Toney; Brian F. Walters

2012-01-01

Wildlife species-habitat matrices are used to relate lists of species with abundance of their habitats. The Forest Inventory and Analysis Program provides data on forest composition and structure, but these attributes may not correspond directly with definitions of wildlife habitats. We used FIA tree data and tree crown diameter models to estimate canopy cover, from...

Rapid classification of landsat TM imagery for phase 1 stratification using the automated NDVI threshold supervised classification (ANTSC) methodology

Treesearch

William H. Cooke; Dennis M. Jacobs

2002-01-01

FIA annual inventories require rapid updating of pixel-based Phase 1 estimates. Scientists at the Southern Research Station are developing an automated methodology that uses a Normalized Difference Vegetation Index (NDVI) for identifying and eliminating problem FIA plots from the analysis. Problem plots are those that have questionable land useiland cover information....

Updating the southern nonnative plant watch list: the future of NNIP Monitoring in the south

Treesearch

Christopher M. Oswalt; Sonja N. Oswalt; Lewis Zimmerman

2012-01-01

The Southern Research Station (SRS) Forest Inventory and Analysis (FIA) Program began monitoring nonnative invasive plant (NNIP) species in 2001 in response to a growing desire to track potential forest health threats on United States forest land. The SRS-FIA NNIP program has produced significant results and contributed considerably to the understanding of the...

Lichens, ozone, and forest health - exploring cross-indicator analyses with FIA data

Treesearch

Susan Will-Wolf; Sarah Jovan

2009-01-01

Does air pollution risk represented by a lichen bioindicator of air pollution, an ozone bioindicator, or a combination of both, correlate with forest health as reflected by condition of tree crowns and other variables? We conducted pilot analyses to answer this question using Forest Inventory and Analysis (FIA) data from the Sierra Nevada region of California and the...

Assessing forest mortality patterns using climate and FIA data at multiple scales

Treesearch

Michael K. Crosby; Zhaofei Fan; Xingang Fan; Theodor D. Leininger; Martin A. Spetich

2012-01-01

Forest Inventory and Analysis (FIA) and PRISM climate data from 1991-2000 were obtained for 10 states in the southeastern United States. Mortality was calculated for each plot, and annual values for precipitation and maximum and minimum temperature were extracted from the PRISM data. Data were then stratified by upland/bottomland for red oak species, and classification...

Landscape Builder: software for the creation of initial landscapes for LANDIS from FIA data

Treesearch

William Dijak

2013-01-01

I developed Landscape Builder to create spatially explicit landscapes as starting conditions for LANDIS Pro 7.0 and LANDIS II landscape forest simulation models from classified satellite imagery and Forest Inventory and Analysis (FIA) data collected over multiple years. LANDIS Pro and LANDIS II models project future landscapes by simulating tree growth, tree species...

A comparison of FIA plot data derived from image pixels and image objects

Treesearch

Charles E. Werstak

2012-01-01

The use of Forest Inventory and Analysis (FIA) plot data for producing continuous and thematic maps of forest attributes (e.g., forest type, canopy cover, volume, and biomass) at the regional level from satellite imagery can be challenging due to differences in scale. Specifically, classification errors that may result from assumptions made between what the field data...

A comparison of carbon stock estimates and projections for the northeastern United States

Treesearch

Richard G. MacLean; Mark J. Ducey; Coeli M. Hoover

2014-01-01

We conducted a comparison of carbon stock estimates produced by three different methods using regional data from the USDA Forest Service Forest Inventory and Analysis (FIA). Two methods incorporated by the Forest Vegetation Simulator (FVS) were compared to each other and to the current FIA component ratio method. We also examined the uncalibrated performance of FVS...

How might FIA deliver more information on status and trends of non-timber forest products?

Treesearch

Stephen P. Prisley

2015-01-01

Data from the Forest Inventory and Analysis program (including the Timber Products Output portion) are critical for assessing the sustainability of US timber production. Private sector users of this information rely on it for strategic planning, and their strong support of the FIA program has helped to ensure funding and program viability. Non-timber forest products...

Forest biomass estimated from MODIS and FIA data in the Lake States: MN, WI and MI, USA

Treesearch

Daolan Zheng; Linda S. Heath; Mark J. Ducey

2007-01-01

This study linked the Moderate Resolution Imaging Spectrometer and USDA Forest Service, Forest Inventory and Analysis (FIA) data through empirical models established using high-resolution Landsat Enhanced Thematic Mapper Plus observations to estimate aboveground biomass (AGB) in three Lake States in the north-central USA. While means obtained from larger sample sizes...

Spatial Information Needs on the Fishlake National Forest: Can FIA Help?

Treesearch

Robert B., Jr. Campbell; Renee A. O' Brien

2005-01-01

National forest staff members are frequently challenged to make assessments with existing information. They rarely have the time or resources to go to the field to gather new data specific to the question at hand. Forest Inventory and Analysis (FIA) data have proved useful in the past, but there is an increasing need for spatial depictions of forest resources to...

Rapid assessment of wildfire damage using Forest Inventory data: A case in Georgia

Treesearch

Richard A. Harper; John W. Coulsten; Jeffery A. Turner

2009-01-01

The rapid assessment of damage caused by natural disasters is essential for planning the appropriate amount of disaster relief funds and public communication. Annual Forest Inventory and Analysis (FIA) data provided initial estimates of damage to timberland in a timely manner to State leaders during the 2007 Georgia Bay Complex Wildfire in southeast Georgia. FIA plots...

Information for forest process models: a review of NRS-FIA vegetation measurements

Treesearch

Charles D. Canham; William H. McWilliams

2012-01-01

The Forest and Analysis Program of the Northern Research Station (NRS-FIA) has re-designed Phase 3 measurements and intensified the sample intensity following a study to balance costs, utility, and sample size. The sampling scheme consists of estimating canopy-cover percent for six vegetation growth habits on 24-foot-radius subplots in four height classes and as an...

Summary and findings of the 2006 BLM Forest Lands Report

Treesearch

Tim Bottomley; Jim Menlove

2009-01-01

In 2006, the Bureau of Land Management (BLM) contracted with the Forest Service Forest Inventory and Analysis Program (FIA) to assist in the preparation of a report specific to all forest lands under the administration of the BLM. The BLM requested that the FIA provide information on the extent and general conditions of BLM- managed forests and woodlands, within...

Comparing alternatives for increasing sampling intensity in forest inventories

Treesearch

J. Blackard; P. Patterson

2014-01-01

Each of the U.S. Forest Service’s Forest Inventory and Analysis (FIA) regions has an occasional need to intensify the national sampling grid. A variety of methodologies exist within the various FIA regions and National Forest Systems regions for constructing plot intensifications, and there is no consensus on a national procedure The primary objectives of this paper...

An assessment of garlic mustard in northern U.S. forests

Treesearch

Cassandra M. Kurtz; Mark H. Hansen

2014-01-01

This publication is part of a series that provides an overview of the presence of invasive plant species monitored on an extensive systematic network of plots measured by the Forest Inventory and Analysis (FIA) program of the U.S. Forest Service, Northern Research Station (NRS). Each research note features one of the invasive plants monitored on forested plots by FIA...

Using FIA inventory plot data to assess NTFP production possibilities

Treesearch

Jobriath Kauffman; James Chamberlain; Stephen Prisley

2015-01-01

The US Forest Service, Forest Inventory and Analysis (FIA) program collects data on a wealth of variables related to trees and understory species in forests. Some of these trees and plants produce non-timber forest products (NTFPs; e.g., seeds, fruit, bark, sap, roots) that are harvested for their culinary and medicinal values. As example, the cones of Pinus...

Alternatives to estimate statewide changes in aspen cover type volumes

Treesearch

Curtis L. VanderSchaaf

2015-01-01

For Minnesota, the only data available to conduct regional or state-wide level assessments across all ownerships is the Forest Inventory and Analysis Program (FIA). Some of the many alternatives available to estimate regional changes in standing volume are referred to here as 1.) FIA alternative, 2.) a commonly applied growth and yield system referred to as Walters and...

Estimating the number of tree species in forest populations using current vegetation survey and forest inventory and analysis approximation plots and grid intensities

Treesearch

Hans T. Schreuder; Jin-Mann S. Lin; John Teply

2000-01-01

We estimate number of tree species in National Forest populations using the nonparametric estimator. Data from the Current Vegetation Survey (CVS) of Region 6 of the USDA Forest Service were used to estimate the number of tree species with a plot close in size to the Forest Inventory and Analysis (FIA) plot and the actual CVS plot for the 5.5 km FIA grid and the 2.7 km...

A randomized phase 2 study of idarubicin and cytarabine with clofarabine or fludarabine in patients with newly diagnosed acute myeloid leukemia.

PubMed

Jabbour, Elias; Short, Nicholas J; Ravandi, Farhad; Huang, Xuelin; Xiao, Lianchun; Garcia-Manero, Guillermo; Plunkett, William; Gandhi, Varsha; Sasaki, Koji; Pemmaraju, Naveen; Daver, Naval G; Borthakur, Gautam; Jain, Nitin; Konopleva, Marina; Estrov, Zeev; Kadia, Tapan M; Wierda, William G; DiNardo, Courtney D; Brandt, Mark; O'Brien, Susan M; Cortes, Jorge E; Kantarjian, Hagop

2017-11-15

Fludarabine and clofarabine are purine nucleoside analogues with established clinical activity in patients with acute myeloid leukemia (AML). Herein, the authors evaluated the efficacy and safety of idarubicin and cytarabine with either clofarabine (CIA) or fludarabine (FIA) in adults with newly diagnosed AML. Adults with newly diagnosed AML who were deemed suitable for intensive chemotherapy were randomized using a Bayesian adaptive design to receive CIA (106 patients) or FIA (76 patients). Patients received induction with idarubicin and cytarabine, plus either clofarabine or fludarabine. Responding patients could receive up to 6 cycles of consolidation therapy. Outcomes were compared with a historical cohort of patients who received idarubicin and cytarabine. The complete remission/complete remission without platelet recovery rate was similar among patients in the CIA and FIA arms (80% and 82%, respectively). The median event-free survival was 13 months and 12 months, respectively (P = .91), and the median overall survival was 24 months and not reached, respectively (P = .23), in the 2 treatment arms. CIA was associated with more adverse events, particularly transaminase elevation, hyperbilirubinemia, and rash. Early mortality was similar in the 2 arms (60-day mortality rate of 4% for CIA vs 1% for FIA; P = .32). In an exploratory analysis of patients aged <50 years, FIA was found to be associated with improved survival compared with idarubicin and cytarabine (2-year event-free survival rate: 58% vs 30% [P = .05] and 2-year overall survival rate: 72% vs 36% [P = .009]). CIA and FIA have similar efficacy in younger patients with newly diagnosed AML, although FIA is associated with a better toxicity profile. Cancer 2017;123:4430-9. © 2017 American Cancer Society. © 2017 American Cancer Society.

A Randomized Phase 2 Study of Idarubicin and Cytarabine With Clofarabine or Fludarabine in Patients With Newly Diagnosed Acute Myeloid Leukemia

PubMed Central

Jabbour, Elias; Short, Nicholas J.; Ravandi, Farhad; Huang, Xuelin; Xiao, Lianchun; Garcia-Manero, Guillermo; Plunkett, William; Gandhi, Varsha; Sasaki, Koji; Pemmaraju, Naveen; Daver, Naval G.; Borthakur, Gautam; Jain, Nitin; Konopleva, Marina; Estrov, Zeev; Kadia, Tapan M.; Wierda, William G.; DiNardo, Courtney D.; Brandt, Mark; O’Brien, Susan M.; Cortes, Jorge E.; Kantarjian, Hagop

2017-01-01

BACKGROUND Fludarabine and clofarabine are purine nucleoside analogues with established clinical activity in patients with acute myeloid leukemia (AML). METHODS Herein, the authors evaluated the efficacy and safety of idarubicin and cytarabine with either clofarabine (CIA) or fludarabine (FIA) in adults with newly diagnosed AML. Adults with newly diagnosed AML who were deemed suitable for intensive chemotherapy were randomized using a Bayesian adaptive design to receive CIA (106 patients) or FIA (76 patients). Patients received induction with idarubicin and cytarabine, plus either clofarabine or fludarabine. Responding patients could receive up to 6 cycles of consolidation therapy. Outcomes were compared with a historical cohort of patients who received idarubicin and cytarabine. RESULTS The complete remission/complete remission without platelet recovery rate was similar among patients in the CIA and FIA arms (80% and 82%, respectively). The median event-free survival was 13 months and 12 months, respectively (P = .91), and the median overall survival was 24 months and not reached, respectively (P = .23), in the 2 treatment arms. CIA was associated with more adverse events, particularly transaminase elevation, hyperbilirubinemia, and rash. Early mortality was similar in the 2 arms (60-day mortality rate of 4% for CIA vs 1% for FIA; P = .32). In an exploratory analysis of patients aged <50 years, FIA was found to be associated with improved survival compared with idarubicin and cytarabine (2-year event-free survival rate: 58% vs 30% [P = .05] and 2-year overall survival rate: 72% vs 36% [P = .009]). CONCLUSIONS CIA and FIA have similar efficacy in younger patients with newly diagnosed AML, although FIA is associated with a better toxicity profile. PMID:28708931

A multi-scale assessment of forest primary production across the eastern USA using Forest Inventory and Analysis (FIA) and MODIS data

NASA Astrophysics Data System (ADS)

Kwon, Youngsang

As evidence of global warming continues to increase, being able to predict the relationship between forest growth rate and climate factors will be vital to maintain the sustainability and productivity of forests. Comprehensive analyses of forest primary production across the eastern US were conducted using remotely sensed MODIS and field-based FIA datasets. This dissertation primarily explored spatial patterns of gross and net carbon uptake in the eastern USA, and addressed three objectives. 1) Examine the use of pixel- and plot-scale screening variables to validate MODIS GPP predictions with Forest Inventory and Analysis (FIA) NPP measures. 2) Assess the net primary production (NPP) from MODIS and FIA at increasing levels of spatial aggregation using a hexagonal tiling system. 3) Assess the carbon use efficiency (CUE) calculated using a direct ratio of MODIS NPP to MODIS GPP and a standardized ratio of FIA NPP to MODIS GPP. The first objective was analyzed using total of 54,969 MODIS pixels and co-located FIA plots to validate MODIS GPP estimates. Eight SVs were used to test six hypotheses about the conditions under which MODIS GPP would be most strongly validated. SVs were assessed in terms of the tradeoff between improved relations and reduced number of samples. MODIS seasonal variation and FIA tree density were the two most efficient SVs followed by basic quality checks for each data set. The sequential application of SVs provided an efficient dataset of 17,090 co-located MODIS pixels and FIA plots, that raised the Pearson's correlation coefficient from 0.01 for the complete dataset of 54,969 plots to 0.48 for this screened subset of 17,090 plots. The second objective was addressed by aggregating data over increasing spatial extents so as to not lose plot- and pixel-level information. These data were then analyzed to determine the optimal scale with which to represent the spatial pattern of NPP. The results suggested an optimal scale of 390 km2. At that scale MODIS and FIA were most strongly correlated while maximizing the number of observation. The maps conveyed both local-scale spatial structure from FIA and broad-scale climatic trends from MODIS. The third objective examined whether carbon use efficiency (CUE) was constant or variable in relation to forest types, and to geographic and climatic variables. The results indicated that while CUEs exhibited unclear patterns by forest types, CUEs are variable to other environmental variables. CUEs are most strongly related to the climatic factors of precipitation followed by temperature. More complex and weaker relationships were found for the geographic factors of latitude and altitude, as they reflected a combination of phenomenological driving forces. The results of the three objectives will help us to identify factors that control carbon cycles and to quantify forest productivity. This will help improve our knowledge about how forest primary productivity may change in relation to ongoing climate change.

Rapid Classification of Landsat TM Imagery for Phase 1 Stratification Using the Automated NDVI Threshold Supervised Classification (ANTSC) Methodology

Treesearch

William H. Cooke; Dennis M. Jacobs

2005-01-01

FIA annual inventories require rapid updating of pixel-based Phase 1 estimates. Scientists at the Southern Research Station are developing an automated methodology that uses a Normalized Difference Vegetation Index (NDVI) for identifying and eliminating problem FIA plots from the analysis. Problem plots are those that have questionable land use/land cover information....

Sampling forest regeneration across northern U.S. forests: filling a void in regeneration model input

Treesearch

William H. McWilliams; Charles D. Canham; Randall S. Morin; Katherine Johnson; Paul Roth; James A. Westfall

2012-01-01

The Forest Inventory and Analysis Program of the Northern Research Station (NRS-FIA) has implemented new Advance Tree Seedling Regeneration (ATSR) protocols that include measurements of seedlings down to 2 inches in height. The addition of ATSR protocols is part of an evaluation of NRS-FIA Phase 3 indicator variables to increase sampling intensity from 1/96,000 acres...

A Manual for the Identification of Invasive Plants in Southern Forests

Treesearch

Lewis Zimmerman

2012-01-01

This manual was created specifically for use by the U.S. Forest Service Southern Research Station (SRS), Forest Inventory and Analysis (FIA) field survey crews. The SRS FIA unit currently collects data on 33 invasive plants or groups across 13 States. The ability to accurately identify plant species in the field is a crucial component of monitoring a species’ presence...

Using interpreted large scale aerial photo data to enhance satellite-based mapping and explore forest land definitions

Treesearch

Tracey S. Frescino; Gretchen G. Moisen

2009-01-01

The Interior-West, Forest Inventory and Analysis (FIA), Nevada Photo-Based Inventory Pilot (NPIP), launched in 2004, involved acquisition, processing, and interpretation of large scale aerial photographs on a subset of FIA plots (both forest and nonforest) throughout the state of Nevada. Two objectives of the pilot were to use the interpreted photo data to enhance...

Potentials for mutually beneficial collaboration between FIA specialists and IEG-40 pathologists and geneticists working on fusiform rust

Treesearch

Ellis Cowling; KaDonna Randolph

2013-01-01

The purpose of this article is to encourage development of an enduring mutually beneficial collaboration between data and information analysts in the US Forest Service’s "Enhanced Forest Inventory and Analysis (FIA) Program" and forest pathologists and geneticists in the information exchange group (IEG) titled "Genetics and Breeding of Southern Forest...

The role of strategic forest inventories in aiding land management decision-making: Examples from the U.S

Treesearch

W. Keith Moser; Renate Bush; John D. Shaw; Mark H. Hansen; Mark D. Nelson

2010-01-01

A major challenge for today’s resource managers is the linking of standand landscape-scale dynamics. The U.S. Forest Service has made major investments in programs at both the stand- (national forest project) and landscape/regional (Forest Inventory and Analysis [FIA] program) levels. FIA produces the only comprehensive and consistent statistical information on the...

Diameter growth models using FIA data from the Northeastern, Southern, and North Central Research Stations

Treesearch

Veronica C. Lessard; Ronald E. McRoberts; Margaret R. Holdaway

2000-01-01

Nonlinear, individual-tree, distance-independent annual diameter growth models are presented for species in two ecoregions defined by R.G. Bailey in the northern Lake States and in parts of the central and southern regions of the U.S. The models were calibrated using Forest Inventory and Analysis (FIA) data from undisturbed plots on land classified as timberland across...

Mortality rates associated with crown health for eastern forest tree species

Treesearch

Randall S. Morin; KaDonna C. Randolph; Jim Steinman

2015-01-01

The condition of tree crowns is an important indicator of tree and forest health. Crown conditions have been evaluated during inventories of the US Forest Service Forest Inventory and Analysis (FIA) program since 1999. In this study, remeasured data from 55,013 trees on 2616 FIA plots in the eastern USA were used to assess the probability of survival among various tree...

A new FIA-Type strategic inventory (NFI)

Treesearch

Richard A. Grotefendt; Hans T. Schreuder

2006-01-01

New remote sensing technologies are now available to lower the cost of doing strategic surveys. A new sampling approach for the Forest Inventory and Analysis program (FIA) of the U.S.D.A. Forest Service is discussed involving a bi-sampling unit (BSU) that is composed of a field sample unit (FSU) centered within a large scale (1:1,000 to 1:3,000) photo sample unit (PSU...

Monitoring nontimber forest products using forest inventory data: an example with slippery elm bark

Treesearch

Jobriath S. Kauffman; Stephen P. Prisley; James L. Chamberlain

2015-01-01

The USDA Forest Service Forest Inventory and Analysi (FIA) program collects data on a wealth of variables related to trees in forests. Some of these trees produce nontimber forest products (NTFPs) (e.g., fruit, bark and sap) that are harvested for culinary, decorative, building, and medicinal purposes. At least 11 tree species inventoried by FIA are valued for their...

The national forest inventory of the United States of America

Treesearch

Ronald E. McRoberts

2008-01-01

The mission of the Forest Inventory and Analysis (FIA) program of the Forest Service, U.S. Department of Agriculture, is to conduct the national forest inventory of the United States of America for purposes of estimating the area of forest land; the volume, growth, and removal of forest resources; and the health of the forest. Users of FIA data, estimates, and related...

Impact of ecological and socioeconomic determinants on the spread of tallow tree in southern forest lands

Treesearch

Yuan Tan; Joseph Z. Fan; Christopher M. Oswalt

2010-01-01

Based on USDA Forest Service Forest Inventory and Analysis (FIA) database, relationships between the presence of tallow tree and related driving variables including forest landscape metrics, stand and site conditions, as well as natural and anthropogenic disturbances were analyzed for the southern states infested by tallow trees. Of the 9,966 re-measured FIA plots in...

Development and Validation of Spatially Explicit Habitat Models for Cavity-nesting Birds in Fishlake National Forest, Utah

Treesearch

Randall A., Jr. Schultz; Thomas C., Jr. Edwards; Gretchen G. Moisen; Tracey S. Frescino

2005-01-01

The ability of USDA Forest Service Forest Inventory and Analysis (FIA) generated spatial products to increase the predictive accuracy of spatially explicit, macroscale habitat models was examined for nest-site selection by cavity-nesting birds in Fishlake National Forest, Utah. One FIA-derived variable (percent basal area of aspen trees) was significant in the habitat...

Trends in standing biomass in Interior West forests: Reassessing baseline data from periodic inventories

Treesearch

Sara A. Goeking

2012-01-01

Trends in U.S. forest biomass and carbon are assessed using Forest Inventory and Analysis (FIA) data relative to baseline assessments from the 1990s. The integrity of baseline data varies by state and depends largely on the comparability of periodic versus annual forest inventory data. In most states in the Interior West FIA region, the periodic inventory's sample...

Miniaturized flow injection analysis system

DOEpatents

Folta, James A.

1997-01-01

A chemical analysis technique known as flow injection analysis, wherein small quantities of chemical reagents and sample are intermixed and reacted within a capillary flow system and the reaction products are detected optically, electrochemically, or by other means. A highly miniaturized version of a flow injection analysis system has been fabricated utilizing microfabrication techniques common to the microelectronics industry. The microflow system uses flow capillaries formed by etching microchannels in a silicon or glass wafer followed by bonding to another wafer, commercially available microvalves bonded directly to the microflow channels, and an optical absorption detector cell formed near the capillary outlet, with light being both delivered and collected with fiber optics. The microflow system is designed mainly for analysis of liquids and currently measures 38.times.25.times.3 mm, but can be designed for gas analysis and be substantially smaller in construction.

Characteristics of pinyon-juniper woodlands in Grand Staircase-Escalante National Monument: Changes since Monument establishment and prospects for future monitoring

Treesearch

Christopher Witt; John D. Shaw

2010-01-01

Recent data from the USDA Forest Service Forest Inventory and Analysis (FIA) program have documented spatial and temporal patterns of drought-related mortality across woodlands of the Southwest (Shaw et al. 2005). In the early 1990s, FIA collected data on forested land now included in Grand Staircase-Escalante National Monument (GSENM or the Monument) as part of a...

Remeasured FIA plots reveal tree-level diameter growth and tree mortality impacts of nitrogen deposition on California’s forests

Treesearch

Mark E. Fenn; Jeremy S. Fried; Haiganoush K. Preisler; Andrzej Bytnerowicz; Susan Schilling; Sarah Jovan; Olaf Kuegler

2015-01-01

The air in California’s forests spans a broad range of purity, from virtually no locally generated pollutants to highly elevated levels of pollutants in forests downwind of urban and agricultural source areas. Ten-year remeasurement data from Forest Inventory and Analysis (FIA) plots in California were used in combination with modelled atmospheric nitrogen (N)...

Baseline results from the Lichen Community Indicator Program in the Pacific Northwest: Air quality patterns and evidence of a nitrogen pollution problem

Treesearch

Sarah Jovan

2009-01-01

Why Are Epiphytic Lichen Communities Important? Lichens are one of the bioindicators used by the Forest Inventory and Analysis (FIA) Program to monitor forest health. To obtain data for use in its Lichen Community Indicator Program, FIA samples a regular network of permanent field plots to determine the composition of epiphytic, i.e., tree dwelling, lichen communities...

The improvement of precision for estimating the abundance of standing dead trees using auxiliary information under the FIA pot design

Treesearch

Hong Su An; David W. MacFarlane; Christopher W. Woodall

2012-01-01

Standing dead trees are an important component of forest ecosystems. However, reliable estimates of standing dead tree population parameters can be difficult to obtain due to their low abundance and spatial and temporal variation. After 1999, the Forest Inventory and Analysis (FIA) Program began collecting data for standing dead trees at the Phase 2 stage of sampling....

The 2014 tanana inventory pilot: A USFS-NASA partnership to leverage advanced remote sensing technologies for forest inventory

Treesearch

Hans-Erik Andersen; Chad Babcock; Robert Pattison; Bruce Cook; Doug Morton; Andrew Finley

2015-01-01

Interior Alaska (approx. 112 million forested acres in size) is the last remaining forested area within the United States where the Forest Inventory and Analysis (FIA) program is not currently implemented. A joint NASA-FIA inventory pilot project was carried out in 2014 to increase familiarity with interior Alaska logistics and evaluate the utility of state-of-the-art...

Placing man in regional landscape classification: Use of Forest Survey data to assess human influences for southern U.S. forest ecosystems

Treesearch

Victor A. Rudis; John B. Tansey

1991-01-01

Information from plots surveyed by U.S.D.A., Forest Service, Forest Inventory and Analysis (FIA) units provides a basis for classifying human-dominated ecosystems at the regional scale of resolution.Attributes include forest stand measures, evidence of human influence, and other disturbances.Data from recent FIA surveys suggest that human influences are common to...

Methods and equations for estimating aboveground volume, biomass, and carbon for trees in the U.S. forest inventory, 2010

Treesearch

Christopher W. Woodall; Linda S. Heath; Grant M. Domke; Michael C. Nichols

2011-01-01

The U.S. Forest Service, Forest Inventory and Analysis (FIA) program uses numerous models and associated coefficients to estimate aboveground volume, biomass, and carbon for live and standing dead trees for most tree species in forests of the United States. The tree attribute models are coupled with FIA's national inventory of sampled trees to produce estimates of...

The role of pre-field operations at four forest inventory units: We can see the trees, not just the forest

Treesearch

Sara A. Goeking; Greg C. Liknes

2009-01-01

The Forest Inventory and Analysis (FIA) program attempts to inventory all forested lands throughout the United States. Each of the four FIA units has developed a process to minimize inventory costs by refraining from visiting those plots in the national inventory grid that are undoubtedly nonforest. We refer to this process as pre-field operations. Until recently, the...

Using SaTScanTM spatial-scan software with national forest inventory data: a case study in South Carolina

Treesearch

KaDonna Randolph

2017-01-01

The USDA Forest Service Forest Inventory and Analysis (FIA) program makes and keeps current an inventory of all forest land in the United States. To comply with privacy laws while at the same time offering its data to the public, FIA makes approximate plot locations available through a process known as perturbing ("fuzzing") and swapping. The free spatial...

Utility of Endoanal Ultrasonography in Assessment of Primary and Recurrent Anal Fistulas and for Detection of Associated Anal Sphincter Defects.

PubMed

Emile, Sameh Hany; Magdy, Alaa; Youssef, Mohamed; Thabet, Waleed; Abdelnaby, Mahmoud; Omar, Waleed; Khafagy, Wael

2017-11-01

Tridimensional endoanal ultrasonography (3D-EAUS) has been used for the assessment of various anorectal lesions. Previous studies have reported good accuracy of 3D-EAUS in preoperative assessment of fistula-in-ano (FIA). This study aimed to assess the diagnostic utility of 3D-EAUS in preoperative evaluation of primary and recurrent FIA and its role in detection of associated anal sphincter (AS) defects. Prospectively collected data of patients with FIA who were investigated with 3D-EAUS were reviewed. The findings of EAUS were compared with the intraoperative findings, the reference standard, to find the degree of agreement regarding the position of the internal opening (IO) and primary tract (PT), and presence of secondary tracts using kappa (k) coefficient test. A subgroup analysis was performed to compare the accuracy and sensitivity of EAUS for primary and recurrent FIA. Of the patients, 131 were included to the study. EAUS had an overall accuracy of 87, 88.5, and 89.5% in detection of IO, PT, and AS defects, respectively. There was very good concordance between the findings of EAUS and intraoperative findings for the investigated parameters (kappa = 0.748, 0.83, 0.935), respectively. Accuracy and sensitivity of EAUS in recurrent FIA were insignificantly lower than primary cases. EAUS detected occult AS defects in 5.3% of the patients studied. The diagnostic utility of 3D-EAUS was comparable in primary and recurrent FIA. 3D-EAUS was able to detect symptomatic and occult AS defects with higher accuracy than clinical examination.

Miniaturized flow injection analysis system

DOEpatents

Folta, J.A.

1997-07-01

A chemical analysis technique known as flow injection analysis is described, wherein small quantities of chemical reagents and sample are intermixed and reacted within a capillary flow system and the reaction products are detected optically, electrochemically, or by other means. A highly miniaturized version of a flow injection analysis system has been fabricated utilizing microfabrication techniques common to the microelectronics industry. The microflow system uses flow capillaries formed by etching microchannels in a silicon or glass wafer followed by bonding to another wafer, commercially available microvalves bonded directly to the microflow channels, and an optical absorption detector cell formed near the capillary outlet, with light being both delivered and collected with fiber optics. The microflow system is designed mainly for analysis of liquids and currently measures 38{times}25{times}3 mm, but can be designed for gas analysis and be substantially smaller in construction. 9 figs.

Aluminum and Manganese Distributions in the Solomon Sea: Results from the 2012 PANDORA Cruise

NASA Astrophysics Data System (ADS)

Michael, S. M.; Resing, J. A.; Jeandel, C.; Lacan, F.

2016-02-01

Much is still unknown about the sources of trace nutrients to the Equatorial Undercurrent (EUC), which ultimately contribute to high-nutrient regions in the Eastern Tropical Pacific. One region that is possibly a source of trace nutrients to the EUC is the Solomon Sea, located east of Papua New Guinea. A study during the summer of 2012, PANDORA, was conducted on board the R/V l'Atalante to determine currents and the geochemical makeup within the basin. Water samples were analyzed for aluminum and manganese using Flow Injection Analysis (FIA). At many stations, aluminum distributions exhibit a sub-surface minimum, located at approximately the same depth as a salinity maximum. Additionally, aluminum is enriched along coastal areas, particularly in the outflow of the Vitiaz Strait, which is concurrent with the findings of Slemons et al. 2010. These regions of high aluminum are also likely regions of iron enrichment. Manganese distributions in the Solomon Sea are similar to data collected north of the region by Slemons et al. 2010, and show a scavenged distribution with local inputs in the surface and concentrations decreasing at depth. This region has strong western boundary currents, and input from coastal margins, two large rivers, island mining sites, and hydrothermal activity, making it an important study-site to determine how trace nutrients are transported to the open ocean.

Thermometric sensing of peroxide in organic media. Application to monitor the stability of RBP-retinol-HRP complex.

PubMed

Ramanathan, K; Jönsson, B R; Danielsson, B

2000-08-01

The stability of horseradish peroxidase (HRP) in aqueous and organic solvents is applied to develop a simple thermometric procedure to detect the binding of retinoic acid-HRP conjugate to retinol binding protein (RBP). Butanone peroxide (BP) in organic phase and hydrogen peroxide in aqueous phase is detected thermometrically on a HRP column, immobilized by cross-linking with glutaraldehyde on controlled pore glass (CPG). Acetone, acetonitrile, methanol, and 2-butanol are used for detection of BP, in the flow injection analysis (FIA) mode. A linear range between 1 and 50 mM BP is obtained in all the organic solvents with a precision of 5-7% (CV%). The magnitude and nature of the thermometric response is significantly different in each organic solvent. The stability of HRP in the organic phase is used to study the stability of a retinoic acid-HRP conjugate bound to immobilized RBP. The response of HRP (to 20 mM BP) in the retinoic acid-HRP conjugate is used as an indicator of the stability of the RBP-retinoic acid-HRP complex, after challenges with various organic/aqueous solvents. Both immobilized HRP and RBP are stable at least for 6 months. The effect of o-phenylene diamine on the thermometric response of HRP is also investigated. A scheme for the design of a thermometric retinol (vitamin A) biosensor is proposed.

Urban FIA: where we have been, where we are, and where we are going

Treesearch

Mark Majewsky

2015-01-01

The FIA program has been inventorying the Nation’s forestland since the 1930s. The focus of the CORE FIA program is to capture trees that meet the FIA definition of forestland, in doing so it excludes trees that do not. Leadership recognized the need to fill this gap and the 2014 Farm Bill has instructed FIA to “Implement an annualized inventory of trees in urban...

Implementing a national process for estimating growth, removals, and mortality at the Pacific Northwest’s Forest Inventory and Analysis’s Region: modeling diameter growth

Treesearch

Olaf. Kuegler

2015-01-01

The Pacific Northwest Research Station’s Forest Inventory and Analysis Unit began remeasurement of permanently located FIA plots under the annualized design in 2011. With remeasurement has come the need to implement the national FIA system for compiling estimates of forest growth, removals, and mortality. The national system requires regional diameter-growth models to...

Unruptured intracranial aneurysms in the Familial Intracranial Aneurysm and International Study of Unruptured Intracranial Aneurysms cohorts: differences in multiplicity and location.

PubMed

Mackey, Jason; Brown, Robert D; Moomaw, Charles J; Sauerbeck, Laura; Hornung, Richard; Gandhi, Dheeraj; Woo, Daniel; Kleindorfer, Dawn; Flaherty, Matthew L; Meissner, Irene; Anderson, Craig; Connolly, E Sander; Rouleau, Guy; Kallmes, David F; Torner, James; Huston, John; Broderick, Joseph P

2012-07-01

Familial predisposition is a recognized nonmodifiable risk factor for the formation and rupture of intracranial aneurysms (IAs). However, data regarding the characteristics of familial IAs are limited. The authors sought to describe familial IAs more fully, and to compare their characteristics with a large cohort of nonfamilial IAs. The Familial Intracranial Aneurysm (FIA) study is a multicenter international study with the goal of identifying genetic and other risk factors for formation and rupture of IAs in a highly enriched population. The authors compared the FIA study cohort with the International Study of Unruptured Intracranial Aneurysms (ISUIA) cohort with regard to patient demographic data, IA location, and IA multiplicity. To improve comparability, all patients in the ISUIA who had a family history of IAs or subarachnoid hemorrhage were excluded, as well as all patients in both cohorts who had a ruptured IA prior to study entry. Of 983 patients enrolled in the FIA study with definite or probable IAs, 511 met the inclusion criteria for this analysis. Of the 4059 patients in the ISUIA study, 983 had a previous IA rupture and 657 of the remainder had a positive family history, leaving 2419 individuals in the analysis. Multiplicity was more common in the FIA patients (35.6% vs 27.9%, p<0.001). The FIA patients had a higher proportion of IAs located in the middle cerebral artery (28.6% vs 24.9%), whereas ISUIA patients had a higher proportion of posterior communicating artery IAs (13.7% vs 8.2%, p=0.016). Heritable structural vulnerability may account for differences in IA multiplicity and location. Important investigations into the underlying genetic mechanisms of IA formation are ongoing.

Deformation sequence of Baltimore gneiss domes, USA, assessed from porphyroblast Foliation Intersection Axes

NASA Astrophysics Data System (ADS)

Yeh, Meng-Wan

2007-05-01

The NE-SW trending gneiss domes around Baltimore, Maryland, USA, have been cited as classic examples of mantled gneiss domes formed by diapiric rise of migmatitic gneisses [Eskola, P., 1949. The problem of mantled gneiss domes. Quarterly Journal of Geological Society of London 104/416, 461-476]. However, 3-D analysis of porphyroblast-matrix foliation relations and porphyroblast inclusion trail geometries suggests that they are the result of interference between multiple refolding of an early-formed nappe. A succession of six FIA (Foliation Intersection Axes) sets, based upon relative timing of inclusion texture in garnet and staurolite porphyroblasts, revealed 6 superposed deformation phases. The successions of inclusion trail asymmetries, formed around these FIAs, document the geometry of deformation associated with folding and fabric development during discrete episodes of bulk shortening. Exclusive top to NW shear asymmetries of curvature were recorded by inclusion trails associated with the vertical collapsing event within the oldest FIA set (NE-SW trend). This strongly indicates a large NE-SW-striking, NW-verging nappe had formed early during this deformation sequence. This nappe was later folded into NE-SW-trending up-right folds by coaxial shortening indicated by an almost equal proportion of both inclusion trail asymmetries documented by the second N-S-trending FIA set. These folds were then amplified by later deformation, as the following FIA sets showed an almost equal proportion of both inclusion trail asymmetries.

DIFFERENTIATION OF AURANTII FRUCTUS IMMATURUS AND FRUCTUS PONICIRI TRIFOLIATAE IMMATURUS BY FLOW-INJECTION WITH ULTRAVIOLET SPECTROSCOPIC DETECTION AND PROTON NUCLEAR MAGNETIC RESONANCE USING PARTIAL LEAST-SQUARES DISCRIMINANT ANALYSIS.

PubMed

Zhang, Mengliang; Zhao, Yang; Harrington, Peter de B; Chen, Pei

2016-03-01

Two simple fingerprinting methods, flow-injection coupled to ultraviolet spectroscopy and proton nuclear magnetic resonance, were used for discriminating between Aurantii fructus immaturus and Fructus poniciri trifoliatae immaturus . Both methods were combined with partial least-squares discriminant analysis. In the flow-injection method, four data representations were evaluated: total ultraviolet absorbance chromatograms, averaged ultraviolet spectra, absorbance at 193, 205, 225, and 283 nm, and absorbance at 225 and 283 nm. Prediction rates of 100% were achieved for all data representations by partial least-squares discriminant analysis using leave-one-sample-out cross-validation. The prediction rate for the proton nuclear magnetic resonance data by partial least-squares discriminant analysis with leave-one-sample-out cross-validation was also 100%. A new validation set of data was collected by flow-injection with ultraviolet spectroscopic detection two weeks later and predicted by partial least-squares discriminant analysis models constructed by the initial data representations with no parameter changes. The classification rates were 95% with the total ultraviolet absorbance chromatograms datasets and 100% with the other three datasets. Flow-injection with ultraviolet detection and proton nuclear magnetic resonance are simple, high throughput, and low-cost methods for discrimination studies.

Tear glucose detection combining microfluidic thread based device, amperometric biosensor and microflow injection analysis.

PubMed

Agustini, Deonir; Bergamini, Márcio F; Marcolino-Junior, Luiz Humberto

2017-12-15

The tear glucose analysis is an important alternative for the indirect, simple and less invasive monitoring of blood glucose levels. However, the high cost and complex manufacturing process of tear glucose analyzers combined with the need to exchange the sensor after each analysis in the disposable tests prevent widespread application of the tear in glucose monitoring. Here, we present the integration of a biosensor made by the electropolymerization of poly(toluidine blue O) (PTB) and glucose oxidase (GOx) with an electroanalytical microfluidic device of easy assembly based on cotton threads, low cost materials and measurements by microflow injection analysis (µFIA) through passive pumping for performing tear glucose analyses in a simple, rapid and inexpensive way. A high stability between the analyses (RSD = 2.54%) and among the different systems (RSD = 3.13%) was obtained for the determination of glucose, in addition to a wide linear range between 0.075 and 7.5mmolL -1 and a limit of detection of 22.2µmolL -1 . The proposed method was efficiently employed in the determination of tear glucose in non-diabetic volunteers, obtaining a close correlation with their blood glucose levels, simplifying and reducing the costs of the analyses, making the tear glucose monitoring more accessible for the population. Copyright © 2017 Elsevier B.V. All rights reserved.

Determination of tannin in green tea infusion by flow-injection analysis based on quenching the fluorescence of 3-aminophthalate.

PubMed

Chen, Richie L C; Lin, Chun-Hsun; Chung, Chien-Yu; Cheng, Tzong-Jih

2005-11-02

A flow-injection analytical system was developed to determine tannin content in green tea infusions. The flow-injection system is based on measuring the quenching effect of tannin on the fluorescence of 3-aminophthalate. Fluorophore was obtained by auto-oxidation of luminol during solution preparation. System performance was satisfactory for routine analysis (sample throughput >20 h(-1); linear dynamic range for tannic acid, 0.005-0.3 mg/mL; linear dynamic range for green tea tannin, 0.02-1.0 mg/mL; CV < 3%). The flow-injection method is immune from interference by coexisting ascorbate in green tea infusion. Analytical results were verified by the ferrous tartrate method, the Japanese official analytical method.

Numerical Simulation of Atomization in Nozzle Injection Flow

NASA Astrophysics Data System (ADS)

Fan, Qinyin; Guo, Chenhai; Takagi, Tosimi; Narumiya, Kikuo; Hattori, Hiroshi

At the initial stage of injection, the injection flow has not yet broken up and in a range of small atmosphere pressure (16˜500KPa), the tip of the injection flow always forms a shape of mushroom. [1] [2] Moreover, the umbrella of the mushroom is always very big and its root is always very thin, especially when the atmosphere pressure is relatively low (88KPa, or 100mmHg). These phenomena are not known popularly and the reason of mushroom formation is not clear. In this paper, with the MARS method for simulating free surface, analysis of injection flow is practiced. The phenomena are reproduced and the reason is cleared that the formation of the mushroom is induced by the momentum exchange between the injection fuel flow with very high speed and the very complex flow of the air.

Chemiluminescence behaviour of CdTe-potassium permanganate enhanced by sodium hexametaphosphate and sensitized sensing of L-ascorbic acid.

PubMed

Chen, Hongqi; Ling, Bo; Yuan, Fei; Zhou, Cailing; Chen, Jingguo; Wang, Lun

2012-01-01

A highly sensitive flow-injection chemiluminescence (FIA-CL) method based on the CdTe nanocrystals and potassium permanganate chemiluminescence system was developed for the determination of L-ascorbic acid. It was found that sodium hexametaphosphate (SP), as an enhancer, could increase the chemiluminescence (CL) emission from the redox reaction of CdTe quantum dots with potassium permanganate in near-neutral pH conditions. L-ascorbic acid is suggested as a sensitive enhancer for use in the above energy-transfer excitation process. Under optimal conditions, the calibration graph of emission intensity against logarithmic l-ascorbic acid concentration was linear in the range 1.0 × 10(-9)-5.0 × 10(-6)  mol/L, with a correlation coefficient of 0.9969 and relative standard deviation (RSD) of 2.3% (n = 7) at 5.0 × 10(-7)  mol/L. The method was successfully used to determine L-ascorbic acid in vitamin C tablets. The possible mechanism of the chemiluminescence in the system is also discussed. Copyright © 2012 John Wiley & Sons, Ltd.

Using GIS to integrate FIA and remotely sensed data to estimate the invasibility of major forest types by non-native invasive plants in the Upper Midwest, USA

Treesearch

Zhaofei Fan; W. Keith Moser; Michael K. Crosby; Weiming Yu

2012-01-01

Non-native invasive plants (NNIP) are rapidly spreading into natural ecosystems such as forests in the Upper Midwest. Using the strategic inventory data from the 2005-2006 U.S. Department of Agriculture, Forest Service’s Forest Inventory and Analysis (FIA) program and forest land cover data, we estimated the regional-invasibility patterns of NNIPs for major...

Determination of Reaction Stoichiometries by Flow Injection Analysis.

ERIC Educational Resources Information Center

Rios, Angel; And Others

1986-01-01

Describes a method of flow injection analysis intended for calculation of complex-formation and redox reaction stoichiometries based on a closed-loop configuration. The technique is suitable for use in undergraduate laboratories. Information is provided for equipment, materials, procedures, and sample results. (JM)

Detection of Catechol by Potentiometric-Flow Injection Analysis in the Presence of Interferents

ERIC Educational Resources Information Center

Lunsford, Suzanne K.; Widera, Justyna; Zhang, Hong

2007-01-01

This article describes an undergraduate analytical chemistry experiment developed to teach instrumental lab skills while incorporating common interferents encountered in the real-world analysis of catechol. The lab technique incorporates potentiometric-flow injection analysis on a dibenzo-18-crown-6 dual platinum electrode to detect catechol in…

Dispersion of a Nanoliter Bolus in Microfluidic Co-Flow.

PubMed

Conway, A J; Saadi, W M; Sinatra, F L; Kowalski, G; Larson, D; Fiering, J

2014-03-01

Microfluidic systems enable reactions and assays on the scale of nanoliters. However, at this scale nonuniformities in sample delivery become significant. To determine the fundamental minimum sample volume required for a particular device, a detailed understanding of mass transport is required. Co-flowing laminar streams are widely used in many devices, but typically only in the steady-state. Because establishing the co-flow steady-state consumes excess sample volume and time, there is a benefit to operating devices in the transient state, which predominates as the volume of the co-flow reactor decreases. Analysis of the co-flow transient has been neglected thus far. In this work we describe the fabrication of a pneumatically controlled microfluidic injector constructed to inject a discrete 50nL bolus into one side of a two-stream co-flow reactor. Using dye for image analysis, injections were performed at a range of flow rates from 0.5-10μL/min, and for comparison we collected the co-flow steady-state data for this range. The results of the image analysis were also compared against theory and simulations for device validation. For evaluation, we established a metric that indicates how well the mass distribution in the bolus injection approximates steady-state co-flow. Using such analysis, transient-state injections can approximate steady-state conditions within predefined errors, allowing straight forward measurements to be performed with reduced reagent consumption.

A 30-meter spatial database for the nation's forests

Treesearch

Raymond L. Czaplewski

2002-01-01

The FIA vision for remote sensing originated in 1992 with the Blue Ribbon Panel on FIA, and it has since evolved into an ambitious performance target for 2003. FIA is joining a consortium of Federal agencies to map the Nation's land cover. FIA field data will help produce a seamless, standardized, national geospatial database for forests at the scale of 30-m...

Real-time measurements of levoglucosan in fine aerosols (PM2.5) in the region of Paris (France)

NASA Astrophysics Data System (ADS)

Sciare, J.; Bonnaire, N.; Mocnik, G.; Nicolas, J.; Petit, J. E.; Bressi, M.; Sarda-Estève, R.; Drinovic, L.

2012-04-01

Levoglucosan - one the major monosaccharide anhydride compounds produced by the combustion of cellulose and hemicellulose - is emitted in large amounts by wildfires or residential wood burning (during winter months). Over the past few years, this specific tracer has received more and more attention at it can be used in a quantitative way to derive atmospheric concentrations of biomass burning aerosols [Favez et al., 2010] which aerosol source has strong implications for climate and air quality studies. A new technique has been developed and is presented here to investigate real-time concentrations of levoglucosan in fine aerosols (PM2.5). This technique is based on a Particle-into-liquid-sampler (PILS, Brechtel Manufacturing inc., model 4002) used "on-line" and coupled with an electrospray ionisation source - tandem mass spectrometry (ESI-MS/MS, AB SCIEX model 3200 QTRAP). Air was drawn in the PILS at 15LPM and removed from particles larger than 2.5μm aerodynamic diameter (AD) using a very sharp cyclone. Water-soluble aerosols were collected in the PILS and sent in the 10μl loop of the ESI-MS/MS at a flowrate of 50μl/min. Flow injection analysis (FIA) was then performed every 2.5min for the quantification of levoglucosan using a specific transition 161-113 m/z (negative mode), by Multiple Reaction Monitoring (MRM) mode. An internal levoglucosan standard was injected every 10 samples (i.e. every 25min) in order to check the stability of the mass spectrometry calibration. Field blanks were performed using a total filter upstream of the PILS instrument and did not show any detectable amount of levoglucosan. A limit of quantification (LOQ) better than 1 ng/m3 was calculated here for levoglucosan in FIA mode. Based on these settings, unattended measurements of levoglucosan by PILS-ESI-MS/MS have been performed every 2.5 min in the region of Paris for a couple of weeks during the winter 2011 showing concentrations ranging from below 1ng/m3 to more than 500ng/m3. These measurements were compared to measurements of Black Carbon from wood burning obtained in parallel every 5 min using a 7 wavelength aethalometer (Magee Scientific, models AE31 and AE33,) and the use of the "aethalometer" model [Sandradewi et al., 2008; Sciare et al., 2001]. A very good agreement was observed between these 2 tracers of biomass burning aerosols, providing valuable information on the strong temporality of the domestic wood burning source. This new tool, in conjunction with other real-time analyzers, may offer new opportunities to investigate the fast atmospheric processes of biomass burning aerosols which have been reported in smog chamber studies [Grieshop et al., 2009].

Application of a novel metabolomic approach based on atmospheric pressure photoionization mass spectrometry using flow injection analysis for the study of Alzheimer's disease.

PubMed

González-Domínguez, Raúl; García-Barrera, Tamara; Gómez-Ariza, José Luis

2015-01-01

The use of atmospheric pressure photoionization is not widespread in metabolomics, despite its considerable potential for the simultaneous analysis of compounds with diverse polarities. This work considers the development of a novel analytical approach based on flow injection analysis and atmospheric pressure photoionization mass spectrometry for rapid metabolic screening of serum samples. Several experimental parameters were optimized, such as type of dopant, flow injection solvent, and their flows, given that a careful selection of these variables is mandatory for a comprehensive analysis of metabolites. Toluene and methanol were the most suitable dopant and flow injection solvent, respectively. Moreover, analysis in negative mode required higher solvent and dopant flows (100 µl min(-1) and 40 µl min(-1), respectively) compared to positive mode (50 µl min(-1) and 20 µl min(-1)). Then, the optimized approach was used to elucidate metabolic alterations associated with Alzheimer's disease. Thereby, results confirm the increase of diacylglycerols, ceramides, ceramide-1-phosphate and free fatty acids, indicating membrane destabilization processes, and reduction of fatty acid amides and several neurotransmitters related to impairments in neuronal transmission, among others. Therefore, it could be concluded that this metabolomic tool presents a great potential for analysis of biological samples, considering its high-throughput screening capability, fast analysis and comprehensive metabolite coverage. Copyright © 2014 Elsevier B.V. All rights reserved.

Analysis of Pooled FIA and Remote Sensing Data for Fiber Supply Assessment at the Warnell School of Forest Resources at the University of Georgia - Other Studies and Effective Information Dissemination

Treesearch

Chris J. Cieszewski; Michael Zasada; Tripp Lowe; Bruce Borders; Mike Clutter; Richard F. Daniels; Robert I. Elle; Robert Izlar; Jarek Zawadzki

2005-01-01

We provide here a short description of the origin, current work, and future outlook of the Fiber Supply Assessment program at the D.B. Warnell School of Forest Resources, University of Georgia, whose work includes various analyses of FIA data. Since 1997, the program has intended to assist the implementation of the new Southern Annual Forest Inventory System through...

Pre-evaluation of metal ions as a catalyst on chemiluminometric sequential injection analysis with luminol-H2O2 system.

PubMed

Takayanagi, Toshio; Inaba, Yuya; Kanzaki, Hiroyuki; Jyoichi, Yasutaka; Motomizu, Shoji

2009-09-15

Catalytic effect of metal ions on luminol chemiluminescence (CL) was investigated by sequential injection analysis (SIA). The SIA system was set up with two solenoid micropumps, an eight-port selection valve, and a photosensor module with a fountain-type chemiluminescence cell. The SIA system was controlled and the CL signals were collected by a LabVIEW program. Aqueous solutions of luminol, H(2)O(2), and a sample solution containing metal ion were sequentially aspirated to the holding coil, and the zones were immediately propelled to the detection cell. After optimizing the parameters using 1 x 10(-5)M Fe(3+) solution, catalytic effect of some metal species was compared. Among 16 metal species examined, relatively strong CL responses were obtained with Fe(3+), Fe(2+), VO(2+), VO(3)(-), MnO(4)(-), Co(2+), and Cu(2+). The limits of detection by the present SIA system were comparable to FIA systems. Permanganate ion showed the highest CL sensitivity among the metal species examined; the calibration graph for MnO(4)(-) was linear at the concentration level of 10(-8)M and the limit of detection for MnO(4)(-) was 4.0 x 10(-10)M (S/N=3).

Flow Rates Measurement and Uncertainty Analysis in Multiple-Zone Water-Injection Wells from Fluid Temperature Profiles

PubMed Central

Reges, José E. O.; Salazar, A. O.; Maitelli, Carla W. S. P.; Carvalho, Lucas G.; Britto, Ursula J. B.

2016-01-01

This work is a contribution to the development of flow sensors in the oil and gas industry. It presents a methodology to measure the flow rates into multiple-zone water-injection wells from fluid temperature profiles and estimate the measurement uncertainty. First, a method to iteratively calculate the zonal flow rates using the Ramey (exponential) model was described. Next, this model was linearized to perform an uncertainty analysis. Then, a computer program to calculate the injected flow rates from experimental temperature profiles was developed. In the experimental part, a fluid temperature profile from a dual-zone water-injection well located in the Northeast Brazilian region was collected. Thus, calculated and measured flow rates were compared. The results proved that linearization error is negligible for practical purposes and the relative uncertainty increases as the flow rate decreases. The calculated values from both the Ramey and linear models were very close to the measured flow rates, presenting a difference of only 4.58 m³/d and 2.38 m³/d, respectively. Finally, the measurement uncertainties from the Ramey and linear models were equal to 1.22% and 1.40% (for injection zone 1); 10.47% and 9.88% (for injection zone 2). Therefore, the methodology was successfully validated and all objectives of this work were achieved. PMID:27420068

Using FIA data to assess current and potential future tree species importance values in the eastern United States

Treesearch

Louis Iverson; Anantha Prasad; Anantha Prasad

2003-01-01

FIA data are extremely valuable for evaluating regional variation in forest distribution. We have processed and summarized FIA data to show four patterns across the Eastern United States: 1) the number and density of FIA forested plots by state, 2) current importance values and frequencies for several species within 20 x 20 km blocks, 3) tree diversity by block, and 4...

Using FIA data to assess current and potential future tree species importance values in the eastern United States

Treesearch

Louis Iverson; Anantha Prasad

2002-01-01

FIA data are extremely valuable for evaluating regional variation in forest distribution. We have processed and summarized FIA data to show four patterns across the Eastern United States: 1) the number and density of FIA forested plots by state, 2) current importance values and frequencies for several species within 20 x 20 km blocks, 3) tree diversity by block, and 4...

The urban FIA inventory: plot design, data collection, data flow and processing

Treesearch

Tonya Lister; Mark Majewsky; Mark A. Hatfield; Angie Rowe; Bill Dunning; Chris Edgar; Tom Brandeis

2015-01-01

More than 80 percent of the U.S. population lives in urban areas and tree cover in these areas offers a wide range of environmental benefits including the provision of wildlife habitat, aesthetic appeal and visual barriers, microclimate control, water quality improvement, and air and noise pollution control. Recognizing the importance of urban forests, and with...

Areas of Agreement and Disagreement Regarding Ponderosa Pine and Mixed Conifer Forest Fire Regimes: A Dialogue with Stevens et al.

PubMed Central

Odion, Dennis C.; Hanson, Chad T.; Baker, William L.; DellaSala, Dominick A.; Williams, Mark A.

2016-01-01

In a recent PLOS ONE paper, we conducted an evidence-based analysis of current versus historical fire regimes and concluded that traditionally defined reference conditions of low-severity fire regimes for ponderosa pine (Pinus ponderosa) and mixed-conifer forests were incomplete, missing considerable variability in forest structure and fire regimes. Stevens et al. (this issue) agree that high-severity fire was a component of these forests, but disagree that one of the several sources of evidence, stand age from a large number of forest inventory and analysis (FIA) plots across the western USA, support our findings that severe fire played more than a minor role ecologically in these forests. Here we highlight areas of agreement and disagreement about past fire, and analyze the methods Stevens et al. used to assess the FIA stand-age data. We found a major problem with a calculation they used to conclude that the FIA data were not useful for evaluating fire regimes. Their calculation, as well as a narrowing of the definition of high-severity fire from the one we used, leads to a large underestimate of conditions consistent with historical high-severity fire. The FIA stand age data do have limitations but they are consistent with other landscape-inference data sources in supporting a broader paradigm about historical variability of fire in ponderosa and mixed-conifer forests than had been traditionally recognized, as described in our previous PLOS paper. PMID:27195808

Areas of Agreement and Disagreement Regarding Ponderosa Pine and Mixed Conifer Forest Fire Regimes: A Dialogue with Stevens et al.

PubMed

Odion, Dennis C; Hanson, Chad T; Baker, William L; DellaSala, Dominick A; Williams, Mark A

2016-01-01

In a recent PLOS ONE paper, we conducted an evidence-based analysis of current versus historical fire regimes and concluded that traditionally defined reference conditions of low-severity fire regimes for ponderosa pine (Pinus ponderosa) and mixed-conifer forests were incomplete, missing considerable variability in forest structure and fire regimes. Stevens et al. (this issue) agree that high-severity fire was a component of these forests, but disagree that one of the several sources of evidence, stand age from a large number of forest inventory and analysis (FIA) plots across the western USA, support our findings that severe fire played more than a minor role ecologically in these forests. Here we highlight areas of agreement and disagreement about past fire, and analyze the methods Stevens et al. used to assess the FIA stand-age data. We found a major problem with a calculation they used to conclude that the FIA data were not useful for evaluating fire regimes. Their calculation, as well as a narrowing of the definition of high-severity fire from the one we used, leads to a large underestimate of conditions consistent with historical high-severity fire. The FIA stand age data do have limitations but they are consistent with other landscape-inference data sources in supporting a broader paradigm about historical variability of fire in ponderosa and mixed-conifer forests than had been traditionally recognized, as described in our previous PLOS paper.

Hydrocarbon group type determination in jet fuels by high performance liquid chromatography

NASA Technical Reports Server (NTRS)

Antoine, A. C.

1977-01-01

Results are given for the analysis of some jet and diesel fuel samples which were prepared from oil shale and coal syncrudes. Thirty-two samples of varying chemical composition and physical properties were obtained. Hydrocarbon types in these samples were determined by fluorescent indicator adsorption (FIA) analysis, and the results from three laboratories are presented and compared. Recently, rapid high performance liquid chromatography (HPLC) methods have been proposed for hydrocarbon group type analysis, with some suggestion for their use as a replacement of the FIA technique. Two of these methods were used to analyze some of the samples, and these results are also presented and compared. Two samples of petroleum-based Jet A fuel are similarly analyzed.

A micro-fluidic sub-microliter sample introduction system for direct analysis of Chinese rice wine by inductively coupled plasma mass spectrometry using external aqueous calibration

NASA Astrophysics Data System (ADS)

Cheng, Heyong; Liu, Jinhua; Xu, Zigang; Yin, Xuefeng

2012-07-01

A microfluidic sub-microliter sample introducing system was developed for direct analysis of Chinese rice wine by inductively coupled plasma mass spectrometry (ICP-MS). It consisted of a microfluidic chip integrating variable-volume sampling channels (0.1-0.8 μL), an eight-way multi-functional valve used in flow injection analysis (FIA), a syringe pump and a peristaltic pump of the Ar ICP-MS instrument. Three solutions, i.e., 15, 40 and 100 g L- 1 glucose in 20% ethanol were used to simulate Chinese rice wine of the dry type, the semidry type and the semisweet type, each. The effects of their volume introduced into ICP-MS on the plasma stability and ICP-MS intensities were studied. The experimental results showed that neither alteration of plasma stability nor carbon deposition was observed when the sampling volume of 20% ethanol containing 100 g L- 1 glucose was downscaled to 0.8 μL. Further reducing the sampling volume to 0.4 μL, no significant difference between the intensities of multi-element standard prepared in three simulated Chinese rice wine matrices and those in aqueous solution was observed. It indicated no negative effect of Chinese rice wine matrix on the ICP-MS intensities. A sampling volume of 0.4 μL was considered to be a good compromise between sensitivity and matrix effect. The flow rate of the carrier was chosen as 20 μL min- 1 for obtaining peaks with the highest peak height within the shortest time. Based on these observations, a microflow injection (μFI) method for the direct determination of cadmium and lead in Chinese rice wine by ICP-MS using an external aqueous calibration was developed. The sample throughput was 45 h- 1 with the detection limit of 19.8 and 10.4 ng L- 1 for Cd and Pb, respectively. The contents of Cd and Pb in 10 Chinese rice wine samples were measured. The results agreed well with those determined by ICP-MS with the conventional sampling system after microwave assisted digestion. The recoveries of three Chinese rice wine samples ranged between 95.5 and 102.8%. Furthermore, the determined contents of Cd and Pb in a certified reference material of red wine (GBW 10031) and a quality control test material of wine (FAPAS T07163QC) by the proposed method were in fairly good accordance with the certified/assigned values. All results verified the accuracy of our method.

Enhanced electrochemiluminescence sensor from tris(2,2'-bipyridyl)ruthenium(II) incorporated into MCM-41 and an ionic liquid-based carbon paste electrode.

PubMed

Li, Jing; Huang, Minghua; Liu, Xiaoqing; Wei, Hui; Xu, Yuanhong; Xu, Guobao; Wang, Erkang

2007-07-01

The electrochemiluminescence (ECL) of tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)(3)(2+)] ion-exchanged in the sulfonic-functionalized MCM-41 silicas was developed with tripropylamine (TPrA) as a co-reactant in a carbon paste electrode (CPE) using a room temperature ionic liquid (IL) as a binder. The sulfonic-functionalized silicas MCM-41 were used for preparing an ECL sensor by the electrostatic interactions between Ru(bpy)(3)(2+) cations and sulfonic acid groups. We used the IL as a binder to construct the CPE (IL-CPE) to replace the traditional binder of the CPE (T-CPE)--silicone oil. The results indicated that the MCM-41-modified IL-CPE had more open structures to allow faster diffusion of Ru(bpy)(3)(2+) and that the ionic liquid also acted as a conducting bridge to connect TPrA with Ru(bpy)(3)(2+) sites immobilized in the electrode, resulting in a higher ECL intensity compared with the MCM-41-modified T-CPE. Herein, the detection limit for TPrA of the MCM-41-modified IL-CPE was 7.2 nM, which was two orders of magnitude lower than that observed at the T-CPE. When this new sensor was used in flow injection analysis (FIA), the MCM-41-modified IL-CPE ECL sensor also showed good reproducibility. Furthermore, the sensor could also be renewed easily by mechanical polishing whenever needed.

Determination of traces of palladium in stream sediment and auto catalyst by FI-ICP-OES using on-line separation and preconcentration with QuadraSil TA.

PubMed

Nakajima, Junichi; Ohno, Masashi; Chikama, Katsumi; Seki, Tatsuya; Oguma, Koichi

2009-09-15

A flow injection analysis (FIA) method using on-line separation and preconcentration with a novel metal scavenger beads, QuadraSil TA, has been developed for the ICP-OES determination of traces of palladium. QuadraSil TA contains diethylenetriamine as a functional group on spherical silica beads and shows the highest selectivity for Pd(II) at pH 1 (0.1 mol l(-1) hydrochloric acid) solution. An aliquot of the sample solution prepared as 0.1 mol l(-1) in hydrochloric acid was passed through the QuadraSil TA column. After washing the column with the carrier solution, the Pd(II) retained on the column was eluted with 0.05 mol l(-1) thiourea solution and the eluate was directly introduced into an ICP-OES. The proposed method was successfully applied to the determination of traces of palladium in JSd-2 stream sediment certified reference material [0.019+/-0.001 microg g(-1) (n=3); provisional value: 0.0212 microg g(-1)] and SRM 2556 used auto catalyst certified reference material [315+/-4 microg g(-1) (n=4); certified value: 326 microg g(-1)]. The detection limit (3 sigma) of 0.28 ng ml(-1) was obtained for 5 ml of sample solution. The sample through puts for 5 ml and 100 microl of the sample solutions were 10 and 15 h(-1), respectively.

On-line ionic liquid-based preconcentration system coupled to flame atomic absorption spectrometry for trace cadmium determination in plastic food packaging materials.

PubMed

Martinis, Estefanía M; Olsina, Roberto A; Altamirano, Jorgelina C; Wuilloud, Rodolfo G

2009-05-15

A novel on-line preconcentration method based on liquid-liquid (L-L) extraction with room temperature ionic liquids (RTILs) coupled to flame atomic absorption spectrometry (FAAS) was developed for cadmium determination in plastic food packaging materials. The methodology is based on the complexation of Cd with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) reagent after sample digestion followed by extraction of the complex with the RTIL 1-butyl-3-methylimidazolium hexafluorophosphate ([C(4)mim][PF(6)]). The mixture was loaded into a flow injection analysis (FIA) manifold and the RTIL rich-phase was retained in a microcolumn filled with silica gel. The RTIL rich-phase was then eluted directly into FAAS. A enhancement factor of 35 was achieved with 20 mL of sample. The limit of detection (LOD), obtained as IUPAC recommendation, was 6 ng g(-1) and the relative standard deviation (R.S.D.) for 10 replicates at 10 microg L(-1) Cd concentration level was 3.9%, calculated at the peak heights. The calibration graph was linear and a correlation coefficient of 0.9998 was achieved. The accuracy of the method was evaluated by both a recovery study and comparison of results with direct determination by electrothermal atomic absorption spectrometry (ETAAS). The method was successfully applied for Cd determination in plastic food packaging materials and Cd concentrations found were in the range of 0.04-10.4 microg g(-1).

Stability Analysis of High-Speed Boundary-Layer Flow with Gas Injection

DTIC Science & Technology

2014-06-01

Vitaly G. Soudakov; Ivett A Leyva 5e. TASK NUMBER 5f. WORK UNIT NUMBER Q0AF 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING...cases of low injection rates in which the N -factors in the near field region are below the critical level, shaping can produce a significant...distribution unlimited Stability analysis of high-speed boundary-layer flow with gas injection Alexander V. Fedorov* and Vitaly G. Soudakov

Stability Analysis of High-Speed Boundary-Layer Flow with Gas Injection (Briefing Charts)

DTIC Science & Technology

2014-06-01

Vitaly G. Soudakov; Ivett A Leyva 5e. TASK NUMBER 5f. WORK UNIT NUMBER Q0AF 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING...cases of low injection rates in which the N -factors in the near field region are below the critical level, shaping can produce a significant...Release; Distribution Unlimited Stability analysis of high-speed boundary-layer flow with gas injection Alexander Fedorov and Vitaly Soudakov Moscow

Head-to-head comparison of the diagnostic accuracies of BD Veritor™ System RSV and Quidel® Sofia® RSV FIA systems for respiratory syncytial virus (RSV) diagnosis.

PubMed

Kanwar, Neena; Hassan, Ferdaus; Nguyen, Ashley; Selvarangan, Rangaraj

2015-04-01

Respiratory syncytial virus (RSV) is one of the most common causes of severe lower respiratory tract disease among infants and young children. BD Veritor™ System RSV (BD) and Quidel(®) Sofia(®) RSV FIA (QD) are the new generation lateral flow digital immunoassay (DIA) tests with an instrumented read for the qualitative detection of RSV viral antigens. To compare the diagnostic accuracies of BD and QD for RSV detection using fresh nasopharyngeal aspirates and nasopharyngeal swab specimens collected in universal transport media during 2013-2014 respiratory season. The two DIA tests were performed simultaneously on randomly selected specimens on a weekly basis during the RSV season until 200 fresh remnant specimens were enrolled. Real-time RT-PCR assay results were used to compare and evaluate the performance of both RSV DIA assays. Among 200 specimens tested, RSV real-time RT-PCR assay detected RSV in 104 samples, while QD detected 84 samples and BD detected 74 samples as positive. The overall sensitivity for detection of RSV in comparison to PCR was 71.15% (61.3-79.4) for BD and 80.77% (71.6-87.6) for QD system (P=0.36). The specificity was 100% (95.2-100) for both systems. The work flow analysis revealed that the overall specimen processing time was significantly lower for BD as compared with the QD assay. In comparison with the real-time PCR, the QD system showed a higher sensitivity than that of the BD system, but the difference did not reach statistical significance (P=0.36). Both BD and QD systems were found comparable in terms of specificity. Copyright © 2015 Elsevier B.V. All rights reserved.

Differentiation of the two major species of Echinacea (E. augustifolia and E. purpurea) using a flow injection mass spectrometric (FIMS) fingerprinting method and chemometric analysis

USDA-ARS?s Scientific Manuscript database

A rapid, simple, and reliable flow-injection mass spectrometric (FIMS) method was developed to discriminate two major Echinacea species (E. purpurea and E. angustifolia) samples. Fifty-eight Echinacea samples collected from United States were analyzed using FIMS. Principle component analysis (PCA) a...

Discrimination of Aurantii Fructus Immaturus and Fructus Poniciri Trifoliatae Immaturus by Flow Injection UV Spectroscopy (FIUV) and 1H NMR using Partial Least-squares Discriminant Analysis (PLS-DA)

USDA-ARS?s Scientific Manuscript database

Two simple fingerprinting methods, flow-injection UV spectroscopy (FIUV) and 1H nuclear magnetic resonance (NMR), for discrimination of Aurantii FructusImmaturus and Fructus Poniciri TrifoliataeImmaturususing were described. Both methods were combined with partial least-squares discriminant analysis...

Coupled Viscous Fluid Flow and Joint Deformation Analysis for Grout Injection in a Rock Joint

NASA Astrophysics Data System (ADS)

Kim, Hyung-Mok; Lee, Jong-Won; Yazdani, Mahmoud; Tohidi, Elham; Nejati, Hamid Reza; Park, Eui-Seob

2018-02-01

Fluid flow modeling is a major area of interest within the field of rock mechanics. The main objective of this study is to gain insight into the performance of grout injection inside jointed rock masses by numerical modeling of grout flow through a single rock joint. Grout flow has been widely simulated using non-Newtonian Bingham fluid characterized by two main parameters of dynamic viscosity and shear yield strength both of which are time dependent. The increasing value of these properties with injection time will apparently affect the parameters representing the grouting performance including grout penetration length and volumetric injection rate. In addition, through hydromechanical coupling a mutual influence between the injection pressure from the one side and the joint opening/closing behavior and the aperture profile variation on the other side is anticipated. This is capable of producing a considerable impact on grout spread within the rock joints. In this study based on the Bingham fluid model, a series of numerical analysis has been conducted using UDEC to simulate the flow of viscous grout in a single rock joint with smooth parallel surfaces. In these analyses, the time-dependent evolution of the grout fluid properties and the hydromechanical coupling have been considered to investigate their impact on grouting performance. In order to verify the validity of these simulations, the results of analyses including the grout penetration length and the injection flow rate were compared with a well-known analytical solution which is available for the simple case of constant grout properties and non-coupled hydraulic analysis. The comparison demonstrated that the grout penetration length can be overestimated when the time-dependent hardening of grout material is not considered. Moreover, due to the HM coupling, it was shown that the joint opening induced by injection pressure may have a considerable increasing impression on the values of penetration length and injected grout volume.

Flow in a porous nozzle with massive wall injection

NASA Technical Reports Server (NTRS)

Kinney, R. B.

1973-01-01

An analytical and experimental investigation has been conducted to determine the effect of massive wall injection on the flow characteristics in a nozzle. The experiments were performed on a water table with a porous-nozzle test section. This had 45 deg and 15 deg half angles of convergence and divergence, respectively, throat radius of 2.5 inches, and throat width of 3 inches. The hydraulic analogy was employed to qualitatively extend the results to a compressible gas flow through the nozzle. An analysis of the water table flow was made using a one-dimensional flow assumption in the continuity and momentum equations. An analysis of a compressible flow in a nozzle was made in a manner analogous to that for the water flow. It is shown that the effect of blowing is to move the sonic position downstream of the geometric throat. Similar results were determined for the incompressible water table flow. Limited photographic results are presented for an injection of air, CO2, and Freon-12 into a main-stream air flow in a convergent-divergent nozzle. Schlieren photographs were used to visualize the flow.

Modeling and flow analysis of pure nylon polymer for injection molding process

NASA Astrophysics Data System (ADS)

Nuruzzaman, D. M.; Kusaseh, N.; Basri, S.; Oumer, A. N.; Hamedon, Z.

2016-02-01

In the production of complex plastic parts, injection molding is one of the most popular industrial processes. This paper addresses the modeling and analysis of the flow process of the nylon (polyamide) polymer for injection molding process. To determine the best molding conditions, a series of simulations are carried out using Autodesk Moldflow Insight software and the processing parameters are adjusted. This mold filling commercial software simulates the cavity filling pattern along with temperature and pressure distributions in the mold cavity. In the modeling, during the plastics flow inside the mold cavity, different flow parameters such as fill time, pressure, temperature, shear rate and warp at different locations in the cavity are analyzed. Overall, this Moldflow is able to perform a relatively sophisticated analysis of the flow process of pure nylon. Thus the prediction of the filling of a mold cavity is very important and it becomes useful before a nylon plastic part to be manufactured.

Analysis of the Electrohydrodynamic Flow in a Symmetric System of Electrodes by the Method of Dynamic Current-Voltage Characteristics

NASA Astrophysics Data System (ADS)

Stishkov, Yu. K.; Zakir'yanova, R. E.

2018-04-01

We have solved the problem of injection-type through electrohydrodynamic (EHD) flow in a closed channel. We have considered a model of a liquid with four types of ions. It is shown that a through EHD flow without internal vortices in the electrode gap is formed for the ratio 2 : 1 of the initial injection current from the electrodes in the channel. The structure of the flow in different parts of the channel and the integral characteristics of the flow have been analyzed. It is shown that for a quadratic function of injection at the electrodes, the current-voltage characteristic of the flow is also quadratic.

Performance of J-33-A-21 and J-33-A-23 Compressors with and without Water Injection

NASA Technical Reports Server (NTRS)

Beede, William L.

1948-01-01

In an investigation of the J-33-A-21 and the J-33-A-23 compressors with and without water injection, it was discovered that the compressors reacted differently to water injection although they were physically similar. An analysis of the effect of water injection on compressor performance and the consequent effect on matching of the compressor and turbine components in the turbojet engine was made. The analysis of component matching is based on a turbine flow function defined as the product of the equivalent weight flow and the reciprocal of the compressor pressure ratio.

Differentiating organic and conventional sage by chromatographic and mass spectrometry flow-injection fingerprints

USDA-ARS?s Scientific Manuscript database

High performance liquid chromatography (UPLC) and flow injection electrospray ionization with ion trap mass spectrometry (FIMS) fingerprints combined with the principal component analysis (PCA) were examined for their potential in differentiating commercial organic and conventional sage samples. The...

Performance and stability analysis of gas-injection enhanced natural circulation in heavy-liquid-metal-cooled systems

NASA Astrophysics Data System (ADS)

Yoo, Yeon-Jong

The purpose of this study is to investigate the performance and stability of the gas-injection enhanced natural circulation in heavy-liquid-metal-cooled systems. The target system is STAR-LM, which is a 400-MWt-class advanced lead-cooled fast reactor under development by Argonne National Laboratory and Oregon State University. The primary loop of STAR-LM relies on natural circulation to eliminate main circulation pumps for enhancement of passive safety. To significantly increase the natural circulation flow rate for the incorporation of potential future power uprates, the injection of noncondensable gas into the coolant above the core is envisioned ("gas lift pump"). Reliance upon gas-injection enhanced natural circulation raises the concern of flow instability due to the relatively high temperature change in the reactor core and the two-phase flow condition in the riser. For this study, the one-dimensional flow field equations were applied to each flow section and the mixture models of two-phase flow, i.e., both the homogeneous and drift-flux equilibrium models were used in the two-phase region of the riser. For the stability analysis, the linear perturbation technique based on the frequency-domain approach was used by employing the Nyquist stability criterion and a numerical root search method. It has been shown that the thermal power of the STAR-LM natural circulation system could be increased from 400 up to 1152 MW with gas injection under the limiting void fraction of 0.30 and limiting coolant velocity of 2.0 m/s from the steady-state performance analysis. As the result of the linear stability analysis, it has turned out that the STAR-LM natural circulation system would be stable even with gas injection. In addition, through the parametric study, it has been found that the thermal inertia effects of solid structures such as fuel rod and heat exchanger tube should be considered in the stability analysis model. The results of this study will be a part of the optimized stable design of the gas-injection enhanced natural circulation of STAR-LM with substantially improved power level and economical competitiveness. Furthermore, combined with the parametric study, this research could contribute a guideline for the design of other similar heavy-liquid-metal-cooled natural circulation systems with gas injection.

Combining FIA plot data with topographic variables: Are precise locations needed?

Treesearch

Stephen P. Prisley; Huei-Jin Wang; Philip J Radtke; John Coulston

2009-01-01

Plot data from the USFS FIA program could be combined with terrain variables to attempt to explain how terrain characteristics influence forest growth, species composition, productivity, fire behavior, wildlife habitat, and other phenomena. While some types of analyses using FIA data have been shown to be insensitive to precision of plot locations, it has been...

Reservoir response to thermal and high-pressure well stimulation efforts at Raft River, Idaho

DOE Office of Scientific and Technical Information (OSTI.GOV)

Plummer, Mitchell; Bradford, Jacob; Moore, Joseph

An injection stimulation test begun at the Raft River geothermal reservoir in June, 2013 has produced a wealth of data describing well and reservoir response via high-resolution temperature logging and distributed temperature sensing, seismic monitoring, periodic borehole televiewer logging, periodic stepped flow rate tests and tracer injections before and after stimulation efforts. One of the primary measures of response to the stimulation is the relationship between fluid pressure and flow rate, short-term during forced flow rate changes and the long-term change in injectivity. In this paper we examine that hydraulic response using standard pumping test analysis methods, largely because pressuremore » response to the stimulation was not detected, or measurable, in other wells. Analysis of stepped rate flow tests supports the inference from other data that a large fracture, with a radial extent of one to several meters, intersects the well in the target reservoir, suggests that the flow regime is radial to a distance of only several meters and demonstrates that the pressure build-up cone reaches an effective constant head at that distance. The well’s longer term hydraulic response demonstrated continually increasing injectivity but at a dramatically faster rate later from ~2 years out and continuing to the present. The net change in injectivity is significantly greater than observed in other longterm injectivity monitoring studies, with an approximately 150–fold increase occurring over ~2.5 years. While gradually increasing injectivity is a likely consequence of slow migration of a cooling front, and consequent dilation of fractures, the steady, ongoing, rate of increase is contrary to what would be expected in a radial or linear flow regime, where the cooling front would slow with time. As a result, occasional step-like changes in injectivity, immediately following high-flow rate tests suggest that hydro shearing during high-pressure testing altered the near-well permeability structure.« less

Regional differences in vitamin D levels and incidence of food-induced anaphylaxis in South Korea.

PubMed

Kim, Si-Heon; Ban, Ga-Young; Park, Hae-Sim; Kim, Su-chin; Ye, Young-Min

2016-03-01

Previous studies have suggested low vitamin D as a potential risk factor for food allergy/anaphylaxis. However, few studies have investigated the association between vitamin D and food-induced anaphylaxis (FIA) in South Korea. To examine regional differences in serum vitamin D levels and FIA incidence. We used nationwide data collected from 2011 to 2013. Data on vitamin D were obtained from the Korea National Health and Nutrition Examination Survey; data on FIA were obtained from the Health Insurance and Assessment Service. Districts were grouped into region 1 (lower solar radiation) and region 2 (higher solar radiation). We examined differences in FIA incidence and vitamin D levels between the regions, adjusting for age. The study included 2,814 patients with FIA and 15,367 people with available serum vitamin D measurements. Age-adjusted FIA incidence was 2.2 per 100,000 person-years in region 1 and 1.8 per 100,000 person-years in region 2 (relative risk, 1.23; 95% confidence interval, 1.09-1.39). Age-adjusted serum vitamin D levels were 16.5 ng/mL in region 1 and 17.8 ng/mL in region 2 (mean difference, 1.3 ng/mL; 95% confidence interval, 0.9-1.9). After stratification by age, sex, and area of residence, region 1 still had higher FIA incidence and lower vitamin D levels than region 2. The present study found a higher incidence of FIA in regions with lower vitamin D levels in the population. Further investigation is necessary to identify any direct associations between vitamin D and food allergy/anaphylaxis. Copyright © 2016 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

Flow-injection analysis of catecholamine secretion from bovine adrenal medulla cells on microbeads.

PubMed

Herrera, M; Kao, L S; Curran, D J; Westhead, E W

1985-01-01

Bovine adrenal medullary cells have been cultured on microbeads which are placed in a low-volume flow system for measurements of stimulation-response parameters. Electronically controlled stream switching allows stimulation of cells with pulse lengths from 1 s to many minutes; pulses may be repeated indefinitely. Catecholamines secreted are detected by an electrochemical detector downstream from the cells. This flow-injection analysis technique provides a new level of sensitivity and precision for measurement of kinetic parameters of secretion. A manual injection valve allows stimulation by higher levels of stimulant in the presence of constant low levels of stimulant. Such experiments show interesting differences between the effects of K+ and acetylcholine on cells partially desensitized to acetylcholine.

Determination of Acidity Constants by Gradient Flow-Injection Titration

ERIC Educational Resources Information Center

Conceicao, Antonio C. L.; Minas da Piedade, Manuel E.

2006-01-01

A three-hour laboratory experiment, designed for an advanced undergraduate course in instrumental analysis that illustrates the application of the gradient chamber flow-injection titration (GCFIT) method with spectrophotometric detection to determine acidity constants is presented. The procedure involves the use of an acid-base indicator to obtain…

The Application of FIA-based Data to Wildlife Habitat Modeling: A Comparative Study

Treesearch

Thomas C., Jr. Edwards; Gretchen G. Moisen; Tracey S. Frescino; Randall J. Schultz

2005-01-01

We evaluated the capability of two types of models, one based on spatially explicit variables derived from FIA data and one using so-called traditional habitat evaluation methods, for predicting the presence of cavity-nesting bird habitat in Fishlake National Forest, Utah. Both models performed equally well, in measures of predictive accuracy, with the FIA-based model...

A statistically valid method for using FIA plots to guide spectral class rejection in producing stratification maps

Treesearch

Michael L. Hoppus; Andrew J. Lister

2002-01-01

A Landsat TM classification method (iterative guided spectral class rejection) produced a forest cover map of southern West Virginia that provided the stratification layer for producing estimates of timberland area from Forest Service FIA ground plots using a stratified sampling technique. These same high quality and expensive FIA ground plots provided ground reference...

Measuring Forest Area Loss Over Time Using FIA Plots and Satellite Imagery

Treesearch

Michael L. Hoppus; Andrew J. Lister

2005-01-01

How accurately can FIA plots, scattered at 1 per 6,000 acres, identify often rare forest land loss, estimated at less than 1 percent per year in the Northeast? Here we explore this question mathematically, empirically, and by comparing FIA plot estimates of forest change with satellite image based maps of forest loss. The mathematical probability of exactly estimating...

A novel combined thermometric and amperometric biosensor for lactose determination based on immobilised cellobiose dehydrogenase.

PubMed

Yakovleva, Maria; Buzas, Orsolya; Matsumura, Hirotoshi; Samejima, Masahiro; Igarashi, Kiyohiko; Larsson, Per-Olof; Gorton, Lo; Danielsson, Bengt

2012-01-15

A novel method for lactose determination in milk is proposed. It is based on oxidation of lactose by cellobiose dehydrogenase (CDH) from the basidiomycete Phanerochaete chrysosporium, immobilised in an enzyme reactor. The reactor was prepared by cross-linking CDH onto aminopropyl-silanised controlled pore glass (CPG) beads using glutaraldehyde. The combined biosensor worked in flow injection analysis (FIA) mode and was developed for simultaneous monitoring of the thermometric signal associated with the enzymatic oxidation of lactose using p-benzoquinone as electron acceptor and the electrochemically generated current associated with the oxidation of the hydroquinone formed. A highly reproducible linear response for lactose was obtained between 0.05 mM and 30 mM. For a set of more than 500 samples an R.S.D. of less than 10% was achieved. The assay time was ca. 2 min per sample. The sensor was applied for the determination of lactose in dairy milk samples (milk with a fat content of 1.5% or 3% and also "lactose free" milk). No sample preparation except dilution with buffer was needed. The proposed method is rapid, suitable for repeated use and allows the possibility to compare results from two different detection methods, thus providing a built-in quality assurance. Some differences in the response observed between the methods indicate that the dual approach can be useful in mechanistic studies of redox enzymes. In addition, a dual system opens up interesting possibilities for studies of enzyme properties and mechanisms. Copyright © 2011 Elsevier B.V. All rights reserved.

Amine oxidase-based biosensors for spermine and spermidine determination.

PubMed

Boffi, Alberto; Favero, Gabriele; Federico, Rodolfo; Macone, Alberto; Antiochia, Riccarda; Tortolini, Cristina; Sanzó, Gabriella; Mazzei, Franco

2015-02-01

The present work describes the development and optimization of electrochemical biosensors for specific determination of the biogenic polyamine spermine (Spm) and spermidine (Spmd) whose assessment represents a novel important analytical tool in food analysis and human diagnostics. These biosensors have been prepared using novel engineered enzymes: polyamine oxidase (PAO) endowed with selectivity towards Spm and Spmd and spermine oxidase (SMO) characterized by strict specificity towards Spm. The current design entails biosensors in which the enzymes were entrapped in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ), a photocrosslinkable gel, onto an electrode surface. Screen-printed electrodes (SPEs) were used as electrochemical transducers for enzymatically produced hydrogen peroxide, operating at different potential vs Ag/AgCl according to the material of the working electrode (WE): +700 mV for graphite (GP) or -100 mV for Prussian blue (PB)-modified SPE, respectively. Biosensor performances were evaluated by means of flow injection amperometric (FIA) measurements. The modified electrodes showed good sensitivity, long-term stability and reproducibility. Under optimal conditions, the PAO biosensor showed a linear range 0.003-0.3 mM for Spm and 0.01-0.4 mM for Spmd, while with the SMO biosensor, a linear range of 0.004-0.5 mM for Spm has been obtained. The main kinetic parameters apparent Michaelis constant (K M), turnover number (K cat) and steady-state current (I max) were determined. The proposed device was then applied to the determination of biogenic amines in blood samples. The results obtained were in good agreement with those obtained with the GC-MS reference method.

Study on the luminescence behavior of sulfobutylether-β-cyclodextrin with risperidone and its analytical application.

PubMed

Wu, Min; Chen, Donghua; Song, Zhenghua

2012-10-01

The interaction of sulfobutylether-β-cyclodextrin (SBE-β-CD) with risperidone (RISP) was first described with luminol-SBE-β-CD chemiluminescence (CL) system by flow injection analysis (FIA). In luminol-SBE-β-CD CL system, the 1:1 SBE-β-CD···luminol(*) complexation could enhance CL intensity of luminol and produce the effect of complexation enhancement of CL (CEC). It was found that RISP could quench the CL intensity of SBE-β-CD···luminol(*) and caused the effect of complexation enhancement of quenching (CEQ), the formation constant K(R-CD) 3.4×10(4) L mol(-1) and the stoichiometric ratio 1:1 of RISP···SBE-β-CD complex were obtained by the proposed CL model. Association degree α 0.036 of RISP···SBE-β-CD complex was also given by CL method. Based on the linear relationship to the decrement of luminol-SBE-β-CD-RISP CL intensity and the logarithm of RISP concentration, RISP also can be quantified in the linear range of 3.0-500.0 nmol L(-1) with a detection limit of 1.0 nmol L(-1) (3σ). The proposed method was successfully applied to monitoring excreted RISP in human urine. It was found that RISP reached its maximum after oral administration for 1.5 h with the total excretion of 14.26% within 8.5 h; the elimination rate constant k and half-life time t(1/2) were 0.474 and 1.5 h, respectively. Copyright © 2012 Elsevier B.V. All rights reserved.

Evaluation of Flow-Injection Tandem Mass Spectrometry for Rapid and High-Throughput Quantitative Determination of B-Vitamins in Nutritional Supplements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhandari, Deepak; Van Berkel, Gary J

2012-01-01

The use of flow-injection electrospray ionization tandem mass spectrometry for rapid and high-throughput mass spectral analysis of selected B-vitamins, viz. B1, B2, B3, B5, and B6, in nutritional formulations was demonstrated. A simple and rapid (~5 min) in-tube sample preparation was performed by adding extraction solvent to a powdered sample aliquot followed by agitation, centrifugation, and filtration to recover an extract for analysis. Automated flow injection introduced 1 L of the extracts directly into the mass spectrometer ion source without chromatographic separation. Sample-to-sample analysis time was 60 s representing significant improvement over conventional liquid chromatography approaches which typically require 25-45more » min, and often require more significant sample preparation procedures. Quantitative capabilities of the flow-injection analysis were tested using the method of standard additions and NIST standard reference material (SRM 3280) multivitamin/multielement tablets. The quantity determined for each B-vitamin in SRM 3280 was within the statistical range provided for the respective certified values. The same sample preparation and analysis approach was also applied to two different commercial vitamin supplement tablets and proved to be successful in the quantification of the selected B-vitamins as evidenced by an agreement with the labels values and the results obtained using isotope dilution liquid chromatography/mass spectrometry.« less

Flow injection method for sulphide determination using an organic mercury compound

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yaqoob, M.; Anwar, M.; Masood, A.S.

1991-04-01

A simple flow injection analysis method is described for the determination of soluble sulfide, based on the complexation of sulfide with p-hydroxymercurbenzoic acid, in the presence of dithizone used as an indicator. The reaction is very rapid, with a sampling rate of 90/hr. and requires a very short length post injection reaction coil. The detection limit and precision are 0.01 mM and 0.7%, respectively.

Parenteral immunization of PLA/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: Evaluation of immunostimulatory action in Labeo rohita (rohu).

PubMed

Rauta, Pradipta Ranjan; Nayak, Bismita

2015-05-01

Advanced vaccine research approaches needs to explore on biodegradable nanoparticles (NPs) based vaccine carrier that can serve as antigen delivery systems as well as immuno-stimulatory action to induce both innate and adaptive immune response in fish. Immunogenicity of PLA and PLGA NPs encapsulating outer membrane protein (Omp) antigen of Aeromonas hydrophila were evaluated through intra-peritoneal injection in fish, Labeo rohita. Antigen loaded PLA-Omp (223.5 ± 13.19 nm) and PLGA-Omp (166.4 ± 21.23 nm) NPs were prepared using double emulsion method by efficiently encapsulating the antigen reaching the encapsulation efficiency 44 ± 4.58% and 59.33 ± 5.13% respectively. Our formulated PLA Omp and PLGA-Omp NPs were in nanometer range (<500 nm) and could be successfully endocyted in the body. Despite low antigen loading in PLA-Omp, it showed considerably slower antigen release in vitro than PLGA-Omp NPs. Other physical properties like zetapotential values and poly dispersity index (PDI) confirmed the stability as well as monodisperse nature of the formulated nanoparticles. The spherical and isolated nature of PLA-Omp and PLGA-Omp NPs were revealed by SEM analysis. Upon immunization of all antigenic formulations (PLA-Omp NP, PLGA-Omp NP, FIA-Omp, PLA NP, PLGA NP, PBS as control), significant higher bacterial agglutination titre and haemolytic activity were observed in case of PLA-Omp and PLGA-Omp immunized groups than rest groups at both 21 days and 42 days. The specific antibody response was significantly increased and persisted up to 42 days of post immunization by PLA-Omp, PLGA-Omp, FIA-Omp. PLA-Omp NPs showed better immune response (higher bacterial agglutination titre, haemolytic activity, specific antibody titre, higher percent survival upon A. hydrophila challenge) than PLGA-Omp in L. rohita confirming its better efficacy. Comparable antibody response of PLA-Omp and PLGA-Omp with FIA-Omp treated groups suggested that PLA and PLGA could be replacement for Freund's adjuvant (for stimulating antibody response) to overcome many side effects offering long lasting immunity. Our encouraging results suggest that PLA/PLGA nanoparticles based delivery system could be a novel antigen carrier for parenteral immunization in fish. Copyright © 2015 Elsevier Ltd. All rights reserved.

Rapid isolation of biomarkers for compound specific radiocarbon dating using high-performance liquid chromatography and flow injection analysis-atmospheric pressure chemical ionisation mass spectrometry.

PubMed

Smittenberg, Rienk H; Hopmans, Ellen C; Schouten, Stefan; Sinninghe Damsté, Jaap S

2002-11-29

Repeated semi-preparative normal-phase HPLC was performed to isolate selected biomarkers from sediment extracts for radiocarbon analysis. Flow injection analysis-mass spectrometry was used for rapid analysis of collected fractions to evaluate the separation procedure, taking only 1 min per fraction. In this way 100-1000 microg of glycerol dialkyl glycerol tetraethers, sterol fractions and chlorophyll-derived phytol were isolated from typically 100 g of marine sediment, i.e., in sufficient quantities for radiocarbon analysis, without significant carbon isotopic fractionation or contamination.

Numerical analysis of combustion characteristics of hybrid rocket motor with multi-section swirl injection

NASA Astrophysics Data System (ADS)

Li, Chengen; Cai, Guobiao; Tian, Hui

2016-06-01

This paper is aimed to analyse the combustion characteristics of hybrid rocket motor with multi-section swirl injection by simulating the combustion flow field. Numerical combustion flow field and combustion performance parameters are obtained through three-dimensional numerical simulations based on a steady numerical model proposed in this paper. The hybrid rocket motor adopts 98% hydrogen peroxide and polyethylene as the propellants. Multiple injection sections are set along the axis of the solid fuel grain, and the oxidizer enters the combustion chamber by means of tangential injection via the injector ports in the injection sections. Simulation results indicate that the combustion flow field structure of the hybrid rocket motor could be improved by multi-section swirl injection method. The transformation of the combustion flow field can greatly increase the fuel regression rate and the combustion efficiency. The average fuel regression rate of the motor with multi-section swirl injection is improved by 8.37 times compared with that of the motor with conventional head-end irrotational injection. The combustion efficiency is increased to 95.73%. Besides, the simulation results also indicate that (1) the additional injection sections can increase the fuel regression rate and the combustion efficiency; (2) the upstream offset of the injection sections reduces the combustion efficiency; and (3) the fuel regression rate and the combustion efficiency decrease with the reduction of the number of injector ports in each injection section.

Elicitors and co-factors in food-induced anaphylaxis in adults

PubMed Central

2013-01-01

Food-induced anaphylaxis (FIA) in adults is often insufficiently diagnosed. One reason is related to the presence of co-factors like exercise, alcohol, additives and non-steroidal anti-inflammatory drugs. The objective of this analysis was to retrospectively investigate the role of co-factors in patients with FIA. 93 adult patients with suspected FIA underwent double-blind, placebo-controlled food challenges with suspected allergens and co-factors. The elicitors of anaphylaxis were identified in 44/93 patients. 27 patients reacted to food allergens upon challenge, 15 patients reacted only when a co-factor was co-exposed with the allergen. The most common identified allergens were celery (n = 7), soy, wheat (n = 4 each) and lupine (n = 3). Among the co-factors food additives (n = 8) and physical exercise (n = 6) were most frequent. In 10 patients more than one co-factor and/or more than one food allergen was necessary to elicit a positive reaction. The implementation of co-factors into the challenge protocol increases the identification rate of elicitors in adult food anaphylactic patients. PMID:24262093

The evolution of Wisconsin's urban FIA program—yesterday today and tomorrow

Treesearch

Andrew M. Stoltman; Richard B. Rideout

2015-01-01

In 2002, Wisconsin was part of two pilot projects in cooperation with the US Forest Service. The first was a street tree assessment, and the second was an urban FIA project. The data generated by these pilots changed the way that Wisconsin DNRs’ Urban Forestry Program conducts its business. Although there have been several urban FIA pilot projects throughout the U.S.,...

Determination of beta-agonists in swine hair by μFIA and chemiluminescence.

PubMed

Chen, Xu; Luo, Yong; Shi, Bo; Gao, Zhigang; Du, Yuguang; Liu, Xianming; Zhao, Weijie; Lin, Bingcheng

2015-04-01

β-Agonists are a group of illegal feed additives. In this paper, it was found that the light emission produced by the oxidation of luminol by potassium ferricyanide was enhanced by the β-agonists (ractopamine, salbutamol, and terbutaline). Based on chemiluminescence phenomenon, a novel, rapid, and sensitive microflow injection analysis system on a microfluidic glass chip was established for determination of the β-agonists. The chip was fabricated from two glass plates (64 mm × 32 mm) with microchannels of 200 μm width and 100 μm depth. The detection limits were achieved at 2.0 × 10(-8) mol/L of ractopamine, 1.0 × 10(-8) mol/L of terbutaline and 5.0 × 10(-7) mol/L of salbutamol. In this report, our method was applied for determination of the β-agonists in swine hair from three different sources with satisfactory results. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Using precursor ion scan of 184 with liquid chromatography-electrospray ionization-tandem mass spectrometry for concentration normalization in cellular lipidomic studies.

PubMed

Chao, Hsi-Chun; Chen, Guan-Yuan; Hsu, Lih-Ching; Liao, Hsiao-Wei; Yang, Sin-Yu; Wang, San-Yuan; Li, Yu-Liang; Tang, Sung-Chun; Tseng, Yufeng Jane; Kuo, Ching-Hua

2017-06-08

Cellular lipidomic studies have been favored approaches in many biomedical research areas. To provide fair comparisons of the studied cells, it is essential to perform normalization of the determined concentration before lipidomic analysis. This study proposed a cellular lipidomic normalization method by measuring the phosphatidylcholine (PC) and sphingomyelin (SM) contents in cell extracts. To provide efficient analysis of PC and SM in cell extracts, flow injection analysis-electrospray ionization-tandem mass spectrometry (FIA-ESI-MS/MS) with a precursor ion scan (PIS) of m/z 184 was used, and the parameters affecting the performance of the method were optimized. Good linearity could be observed between the cell extract dilution factor and the reciprocal of the total ion chromatogram (TIC) area in the PIS of m/z 184 within the dilution range of 1- to 16-fold (R 2  = 0.998). The calibration curve could be used for concentration adjustment of the unknown concentration of a cell extract. The intraday and intermediate precisions were below 10%. The accuracy ranged from 93.0% to 105.6%. The performance of the new normalization method was evaluated using different numbers of HCT-116 cells. Sphingosine, ceramide (d18:1/18:0), SM (d18:1/18:0) and PC (16:1/18:0) were selected as the representative test lipid species, and the results showed that the peak areas of each lipid species obtained from different cell numbers were within a 20% variation after normalization. Finally, the PIS of 184 normalization method was applied to study ischemia-induced neuron injury using oxygen and glucose deprivation (OGD) on primary neuronal cultured cells. Our results showed that the PIS of 184 normalization method is an efficient and effective approach for concentration normalization in cellular lipidomic studies. Copyright © 2017 Elsevier B.V. All rights reserved.

Evaluation of total phenol pollution in water of San Martin Canal from Santiago del Estero, Argentina.

PubMed

Acosta, C A; Pasquali, C E López; Paniagua, G; Garcinuño, R M; Hernando, P Fernández

2018-05-01

Santiago del Estero is a province located in northwestern Argentina. The Dulce River is used for irrigation through a vast network of channels and ditches, including the San Martin Canal (SMC), which crosses the capital city of Santiago del Estero. This canal's water is used for drinking, as well as recreational use for the general population. However, this river has been seriously polluted for several decades. The present study focuses on the identification and the quantification of the water pollution levels of total phenols in the SMC according to the seasonal periods. Water samples from various areas of the canal in different months of the year, extending from December to September, were collected for analysis. Additionally, the concentration of total dissolved solids (TDS), chlorides, sulphates, nitrites and organic matter, as well as water hardness and alkalinity, were analysed in order to conduct a more complete study of the contamination of this area. The results showed a worrying total phenol concentration that exceeded the limit set by Argentine legislation for drinking water, as well as water for recreational use (5 μg/L). The total phenol (TP) concentration was directly determined by a molecular absorption spectroscopy method based on a new flow injection analysis system (FIA). Under the selected experimental conditions, the detection and quantification limits were 0.0490 and 0.1633 μg/mL, respectively. The developed method provides a number of improvements related to the speed of analysis, the restricted consumption of the reagents and sample volumes and the unnecessary sample treatment that contribute to environmentally friendly analytical chemistry. The results showed that TP make a significant contribution in the SMC pollution, especially during the months of April (400 ± 110 μg/L) and September (240 ± 20 μg/L). A high sulphate concentration that was higher than the limit allowed by the legislation was also found. Copyright © 2018 Elsevier Ltd. All rights reserved.

Comparison of Series of Vugs and Non-vuggy Synthetic Porous Media on Formation Damage

NASA Astrophysics Data System (ADS)

Khan, H.; DiCarlo, D. A.; Prodanovic, M.

2017-12-01

Produced water reinjection (PWRI) is an established cost-effective oil field practice where produced water is injected without any cleanup, for water flooding or disposal. Resultantly the cost of fresh injection fluid and/or processing produced water is saved. A common problem with injection of unprocessed water is formation damage in the near injection zone due to solids (fines) entrapment, causing a reduction in permeability and porosity of the reservoir. Most studies have used homogeneous porous media with unimodal grain sizes, while real world porous media often has a wide range of pores, up to and including vugs in carbonaceous rocks. Here we fabricate a series of vugs in synthetic porous media by sintering glass beads with large dissolvable inclusions. The process is found to be repeatable, allowing a similar vug configuration to be tested for different flow conditions. Bi-modal glass bead particles (25 & 100 micron) are injected at two different flow rates and three different injection concentrations. Porosity, permeability and effluent concentration are determined using CT scanning, pressure measurements and particle counting (Coulter counter), respectively. Image analysis is performed on the CT images to determine the change in vug size for each flow condition. We find that for the same flow conditions, heterogeneous media with series of vugs have an equal or greater permeability loss compared to homogeneous porous media. A significant change in permeability is observed at the highest concentration and flow rate as more particles approach the filter quickly, resulting in a greater loss in permeability in the lower end of the core. Image analysis shows the highest loss in vug size occurs at the low flow rate and highest concentration. The lower vug is completely blocked for this flow case. For all flow cases lower values of porosity are observed after the core floods. At low flow rate and medium concentration, a drastic loss in porosity is observed in the lower part of the core, after the vuggy zone. This trough is also distinctly clear in the homogeneous core for the same flow conditions. This study focuses on understanding the effect of pore heterogeneity on formation damage. We conclude that more damage is done deeper in vuggy formations at high flow rates, resulting in shorter injection cycle prior to clean up.

A two-dimensional cascade solution using minimized surface singularity density distributions - with application to film cooled turbine blades

NASA Technical Reports Server (NTRS)

Mcfarland, E.; Tabakoff, W.; Hamed, A.

1977-01-01

An investigation of the effects of coolant injection on the aerodynamic performance of cooled turbine blades is presented. The coolant injection is modeled in the inviscid irrotational adiabatic flow analysis through the cascade using the distributed singularities approach. The resulting integral equations are solved using a minimized surface singularity density criteria. The aerodynamic performance was evaluated using this solution in conjunction with an existing mixing theory analysis. The results of the present analysis are compared with experimental measurements in cold flow tests.

Quantitation of glycerophosphorylcholine by flow injection analysis using immobilized enzymes.

PubMed

Mancini, A; Del Rosso, F; Roberti, R; Caligiana, P; Vecchini, A; Binaglia, L

1996-09-20

A method for quantitating glycerophosphorylcholine by flow injection analysis is reported in the present paper. Glycerophosphorylcholine phosphodiesterase and choline oxidase, immobilized on controlled porosity glass beads, are packed in a small reactor inserted in a flow injection manifold. When samples containing glycerophosphorylcholine are injected, glycerophosphorylcholine is hydrolyzed into choline and sn-glycerol-3-phosphate. The free choline produced in this reaction is oxidized to betain and hydrogen peroxide. Hydrogen peroxide is detected amperometrically. Quantitation of glycerophosphorylcholine in samples containing choline and phosphorylcholine is obtained inserting ahead of the reactor a small column packed with a mixed bed ion exchange resin. The time needed for each determination does not exceed one minute. The present method, applied to quantitate glycerophosphorylcholine in samples of seminal plasma, gave results comparable with those obtained using the standard enzymatic-spectrophotometric procedure. An alternative procedure, making use of co-immobilized glycerophosphorylcholine phosphodiesterase and glycerol-3-phosphate oxidase for quantitating glycerophosphorylcholine, glycerophosphorylethanolamine and glycerophosphorylserine is also described.

Experiment T001: Entry communication on the Gemini 3 mission

NASA Technical Reports Server (NTRS)

Schroeder, L. C.; Sims, T. E.; Cuddihy, W. F.

1971-01-01

Water addition to the Gemini 3 exhaust plasma was studied to determine its effectiveness in the establishment of communication links during the entry portion of the flight. Attenuation levels were measured with and without water injection at uhf frequencies of 230.4 and 296.8 megahertz and at the C-band frequency of 5690 megahertz. Ultrahigh frequency signals that had been blacked out were restored to significant levels, during early portions of the water-injection sequence, by the high flow rate injection. The C-band signal was enhanced by medium and high flow rate injections during the latter portion of the injection period. The uhf signal recovered during water injection resulted in an antenna pattern that was beamed in the radial direction of injection from the spacecraft. Postflight analysis showed that the uhf recovery data were consistent with injection-penetration theory.

Effect of lipoarabinomannan from Mycobacterium avium subsp avium in Freund's incomplete adjuvant on the immune response of cattle.

PubMed

Colavecchia, S B; Jolly, A; Fernández, B; Fontanals, A M; Fernández, E; Mundo, S L

2012-02-01

The aim of the present study was to determine whether lipoarabinomannan (LAM), in combination with Freund's incomplete adjuvant (FIA), was able to improve cell-mediated and antibody-mediated immune responses against ovalbumin (OVA) in cattle. Twenty-three calves were assigned to four treatment groups, which were subcutaneously immunized with either OVA plus FIA, OVA plus FIA and LAM from Mycobacterium avium subsp avium, FIA plus LAM, or FIA alone. Lymphoproliferation, IFN-γ production and cell subpopulations on peripheral blood mononuclear cells before and 15 days after treatment were evaluated. Delayed hypersensitivity was evaluated on day 57. Specific humoral immune response was measured by ELISA. Inoculation with LAM induced higher levels of lymphoproliferation and IFN-γ production in response to ConA and OVA (P < 0.05). Specific antibody titers were similar in both OVA-immunized groups. Interestingly, our results showed that the use of LAM in vaccine preparations improved specific cell immune response evaluated by lymphoproliferation and IFN-γ production by at least 50 and 25%, respectively, in cattle without interfering with tuberculosis and paratuberculosis diagnosis.

Effect of lipoarabinomannan from Mycobacterium avium subsp avium in Freund's incomplete adjuvant on the immune response of cattle

PubMed Central

Colavecchia, S.B.; Jolly, A.; Fernández, B.; Fontanals, A.M.; Fernández, E.; Mundo, S.L.

2012-01-01

The aim of the present study was to determine whether lipoarabinomannan (LAM), in combination with Freund's incomplete adjuvant (FIA), was able to improve cell-mediated and antibody-mediated immune responses against ovalbumin (OVA) in cattle. Twenty-three calves were assigned to four treatment groups, which were subcutaneously immunized with either OVA plus FIA, OVA plus FIA and LAM from Mycobacterium avium subsp avium, FIA plus LAM, or FIA alone. Lymphoproliferation, IFN-γ production and cell subpopulations on peripheral blood mononuclear cells before and 15 days after treatment were evaluated. Delayed hypersensitivity was evaluated on day 57. Specific humoral immune response was measured by ELISA. Inoculation with LAM induced higher levels of lymphoproliferation and IFN-γ production in response to ConA and OVA (P < 0.05). Specific antibody titers were similar in both OVA-immunized groups. Interestingly, our results showed that the use of LAM in vaccine preparations improved specific cell immune response evaluated by lymphoproliferation and IFN-γ production by at least 50 and 25%, respectively, in cattle without interfering with tuberculosis and paratuberculosis diagnosis. PMID:22286534

Effects of mold design of aspheric projector lens for head up display

NASA Astrophysics Data System (ADS)

Chen, Chao-Chang A.; Tang, Jyun-Cing; Teng, Lin-Ming

2010-08-01

This paper investigates the mold design and related effects on an aspheric projector lens for Head Up Display (HUD) with injection molding process. Injection flow analysis with a commercial software, Moldex3D has been used to simulate this projector lens for filling, packing, shrinkage, and flow-induced residual stress. This projector lens contains of variant thickness due to different aspheric design on both surfaces. Defects may be induced as the melt front from the gate into the cavity with jet-flow phenomenon, short shot, weld line, and even shrinkage. Thus, this paper performs a gate design to find the significant parameters including injection velocity, melt temperature, and mold temperature. After simulation by the Moldex3D, gate design for the final assembly of Head Up Display (HUD) has been obtained and then experimental tests have been proceeded for verification of short-shot, weight variation, and flow-induced stress. Moreover, warpage analysis of the Head Up Display (HUD) can be integrated with the optical design specification in future work.

Rapid Quantitation of Ascorbic and Folic Acids in SRM 3280 Multivitamin/Multielement Tablets using Flow-Injection Tandem Mass Spectrometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhandari, Deepak; Kertesz, Vilmos; Van Berkel, Gary J

RATIONALE: Ascorbic acid (AA) and folic acid (FA) are water-soluble vitamins and are usually fortified in food and dietary supplements. For the safety of human health, proper intake of these vitamins is recommended. Improvement in the analysis time required for the quantitative determination of these vitamins in food and nutritional formulations is desired. METHODS: A simple and fast (~5 min) in-tube sample preparation was performed, independently for FA and AA, by mixing extraction solvent with a powdered sample aliquot followed by agitation, centrifugation, and filtration to recover an extract for analysis. Quantitative detection was achieved by flow-injection (1 L injectionmore » volume) electrospray ionization tandem mass spectrometry (ESI-MS/MS) in negative ion mode using the method of standard addition. RESULTS: Method of standard addition was employed for the quantitative estimation of each vitamin in a sample extract. At least 2 spiked and 1 non-spiked sample extract were injected in triplicate for each quantitative analysis. Given an injection-to-injection interval of approximately 2 min, about 18 min was required to complete the quantitative estimation of each vitamin. The concentration values obtained for the respective vitamins in the standard reference material (SRM) 3280 using this approach were within the statistical range of the certified values provided in the NIST Certificate of Analysis. The estimated limit of detections of FA and AA were 13 and 5.9 ng/g, respectively. CONCLUSIONS: Flow-injection ESI-MS/MS was successfully applied for the rapid quantitation of FA and AA in SRM 3280 multivitamin/multielement tablets.« less

National-scale aboveground biomass geostatistical mapping with FIA inventory and GLAS data: Preparation for sparsely sampled lidar assisted forest inventory

NASA Astrophysics Data System (ADS)

Babcock, C. R.; Finley, A. O.; Andersen, H. E.; Moskal, L. M.; Morton, D. C.; Cook, B.; Nelson, R.

2017-12-01

Upcoming satellite lidar missions, such as GEDI and IceSat-2, are designed to collect laser altimetry data from space for narrow bands along orbital tracts. As a result lidar metric sets derived from these sources will not be of complete spatial coverage. This lack of complete coverage, or sparsity, means traditional regression approaches that consider lidar metrics as explanatory variables (without error) cannot be used to generate wall-to-wall maps of forest inventory variables. We implement a coregionalization framework to jointly model sparsely sampled lidar information and point-referenced forest variable measurements to create wall-to-wall maps with full probabilistic uncertainty quantification of all inputs. We inform the model with USFS Forest Inventory and Analysis (FIA) in-situ forest measurements and GLAS lidar data to spatially predict aboveground forest biomass (AGB) across the contiguous US. We cast our model within a Bayesian hierarchical framework to better model complex space-varying correlation structures among the lidar metrics and FIA data, which yields improved prediction and uncertainty assessment. To circumvent computational difficulties that arise when fitting complex geostatistical models to massive datasets, we use a Nearest Neighbor Gaussian process (NNGP) prior. Results indicate that a coregionalization modeling approach to leveraging sampled lidar data to improve AGB estimation is effective. Further, fitting the coregionalization model within a Bayesian mode of inference allows for AGB quantification across scales ranging from individual pixel estimates of AGB density to total AGB for the continental US with uncertainty. The coregionalization framework examined here is directly applicable to future spaceborne lidar acquisitions from GEDI and IceSat-2. Pairing these lidar sources with the extensive FIA forest monitoring plot network using a joint prediction framework, such as the coregionalization model explored here, offers the potential to improve forest AGB accounting certainty and provide maps for post-model fitting analysis of the spatial distribution of AGB.

The forest inventory and analysis program: what's in it for landowners?

Treesearch

Richard Harper

2009-01-01

In 1597, Sir Francis Bacon coined the phrase, "Knowledge is power." KnOwledge today often means knowing where to find reliable information from branded research sources. One such source that benefits forest landowners and the forest community is the Forest Inventory and Analysis (FIA) program.

Forest resources of the Tonto National Forest

Treesearch

John D. Shaw

2004-01-01

The Interior West Forest Inventory and Analysis (IWFIA) program of the USDA Forest Service, Rocky Mountain Research Station, as part of its national Forest Inventory and Analysis (FIA) duties, conducted forest resource inventories of the Southwestern Region (Region 3) National Forests. This report presents highlights of the Tonto National Forest 1996 inventory...

Forest resources of the Prescott National Forest

Treesearch

Paul Rogers

2003-01-01

The Interior West Forest Inventory and Analysis (IWFIA) program of the USDA Forest Service, Rocky Mountain Research Station, as part of its national Forest Inventory and Analysis (FIA) duties, conducted forest resource inventories of the Southwestern Region (Region 3) National Forests. This report presents highlights of the Prescott National Forest 1996...

Forest resources of the Lincoln National Forest

Treesearch

John D. Shaw

2006-01-01

The Interior West Forest Inventory and Analysis (IWFIA) program of the USDA Forest Service, Rocky Mountain Research Station, as part of its national Forest Inventory and Analysis (FIA) duties, conducted forest resource inventories of the Southwestern Region (Region 3) National Forests. This report presents highlights of the Lincoln National Forest 1997 inventory...

The reporting revolution—the southern endeavor

Treesearch

Mark Brown

2009-01-01

The need for expeditious portrayal of statewide inventory findings is paramount. Demand is intensifying. Yet, to date, relaying data results and analysis through traditional publications has been extremely time consuming. To address this issue, southern forest inventory and analysis (FIA) reporting is in transition. This article discusses the evolution of authorship,...