10 CFR 429.26 - Fluorescent lamp ballasts.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 3 2013-01-01 2013-01-01 false Fluorescent lamp ballasts. 429.26 Section 429.26 Energy... COMMERCIAL AND INDUSTRIAL EQUIPMENT Certification § 429.26 Fluorescent lamp ballasts. (a) Sampling plan for selection of units for testing. (1) The requirements of § 429.11 are applicable to fluorescent lamp ballasts...

30 CFR 75.522-1 - Incandescent and fluorescent lamps.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Incandescent and fluorescent lamps. 75.522-1...-1 Incandescent and fluorescent lamps. (a) Except for areas of a coal mine inby the last open crosscut, incandescent lamps may be used to illuminate underground areas. When incandescent lamps are used...

30 CFR 75.522-1 - Incandescent and fluorescent lamps.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Incandescent and fluorescent lamps. 75.522-1...-1 Incandescent and fluorescent lamps. (a) Except for areas of a coal mine inby the last open crosscut, incandescent lamps may be used to illuminate underground areas. When incandescent lamps are used...

30 CFR 75.522-1 - Incandescent and fluorescent lamps.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Incandescent and fluorescent lamps. 75.522-1...-1 Incandescent and fluorescent lamps. (a) Except for areas of a coal mine inby the last open crosscut, incandescent lamps may be used to illuminate underground areas. When incandescent lamps are used...

30 CFR 75.522-1 - Incandescent and fluorescent lamps.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Incandescent and fluorescent lamps. 75.522-1...-1 Incandescent and fluorescent lamps. (a) Except for areas of a coal mine inby the last open crosscut, incandescent lamps may be used to illuminate underground areas. When incandescent lamps are used...

30 CFR 75.522-1 - Incandescent and fluorescent lamps.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Incandescent and fluorescent lamps. 75.522-1...-1 Incandescent and fluorescent lamps. (a) Except for areas of a coal mine inby the last open crosscut, incandescent lamps may be used to illuminate underground areas. When incandescent lamps are used...

340nm UV LED excitation in time-resolved fluorescence system for europium-based immunoassays detection

NASA Astrophysics Data System (ADS)

Rodenko, Olga; Fodgaard, Henrik; Tidemand-Lichtenberg, Peter; Pedersen, Christian

2017-02-01

In immunoassay analyzers for in-vitro diagnostics, Xenon flash lamps have been widely used as excitation light sources. Recent advancements in UV LED technology and its advantages over the flash lamps such as smaller footprint, better wall-plug efficiency, narrow emission spectrum, and no significant afterglow, have made them attractive light sources for gated detection systems. In this paper, we report on the implementation of a 340 nm UV LED based time-resolved fluorescence system based on europium chelate as a fluorescent marker. The system performance was tested with the immunoassay based on the cardiac marker, TnI. The same signal-to-noise ratio as for the flash lamp based system was obtained, operating the LED below specified maximum current. The background counts of the system and its main contributors were measured and analyzed. The background of the system of the LED based unit was improved by 39% compared to that of the Xenon flash lamp based unit, due to the LEDs narrower emission spectrum and longer pulse width. Key parameters of the LED system are discussed to further optimize the signal-to-noise ratio and signal-to-background, and hence the sensitivity of the instrument.

Environmental impacts of lighting technologies - Life cycle assessment and sensitivity analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Welz, Tobias; Hischier, Roland, E-mail: Roland.Hischier@empa.ch; Hilty, Lorenz M.

2011-04-15

With two regulations, 244/2009 and 245/2009, the European Commission recently put into practice the EuP Directive in the area of lighting devices, aiming to improve energy efficiency in the domestic lighting sector. This article presents a comprehensive life cycle assessment comparison of four different lighting technologies: the tungsten lamp, the halogen lamp, the conventional fluorescent lamp and the compact fluorescent lamp. Taking advantage of the most up-to-date life cycle inventory database available (ecoinvent data version 2.01), all life cycle phases were assessed and the sensitivity of the results for varying assumptions analysed: different qualities of compact fluorescent lamps (production phase),more » different electricity mixes (use phase), and end-of-life scenarios for WEEE recycling versus municipal solid waste incineration (disposal phase). A functional unit of 'one hour of lighting' was defined and the environmental burdens for the whole life cycle for all four lamp types were calculated, showing a clearly lower impact for the two gas-discharge lamps, i.e. the fluorescent and the compact fluorescent lamp. Differences in the product quality of the compact fluorescent lamps reveal to have only a very small effect on the overall environmental performance of this lamp type; a decline of the actual life time of this lamp type doesn't result in a change of the rank order of the results of the here examined four lamp types. It was also shown that the environmental break-even point of the gas-discharge lamps is reached long before the end of their expected life-span. All in all, it can be concluded that a change from today's tungsten lamp technology to a low-energy-consuming technology such as the compact fluorescent lamp results in a substantial environmental benefit.« less

78 FR 14357 - Certain Compact Fluorescent Reflector Lamps, Products Containing Same and Components Thereof...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-05

... Reflector Lamps, Products Containing Same and Components Thereof; Institution of Investigation AGENCY: U.S... fluorescent reflector lamps, products containing same and components thereof by reason of infringement of... compact fluorescent reflector lamps, products containing same and components thereof by reason of...

Fluorescent and high intensity discharge lamp use in chambers and greenhouses

NASA Technical Reports Server (NTRS)

Langhans, Robert W.

1994-01-01

Fluorescent and High Intensity Discharge lamps have opened up great opportunities for researchers to study plant growth under controlled environment conditions and for commercial growers to increase plant production during low/light periods. Specific technical qualities of fluorescent and HID lamps have been critically reviewed. I will direct my remarks to fluorescent and high intensity discharge (HID) lamps in growth chambers, growth rooms, and greenhouses. I will discuss the advantages and disadvantages of using each lamp in growth chambers, growth rooms and greenhouses.

10 CFR Appendix Q1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2014 CFR

2014-01-01

... of Fluorescent Lamp Ballasts Q1 Appendix Q1 to Subpart B of Part 430 Energy DEPARTMENT OF ENERGY... of Fluorescent Lamp Ballasts Comply with Appendix Q1 beginning November 14, 2014. Prior to this date, all fluorescent lamp ballasts shall be tested using the provisions of Appendix Q. 1. Definitions 1.1...

10 CFR Appendix Q1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2013 CFR

2013-01-01

... of Fluorescent Lamp Ballasts Q1 Appendix Q1 to Subpart B of Part 430 Energy DEPARTMENT OF ENERGY... of Fluorescent Lamp Ballasts Comply with Appendix Q1 beginning November 14, 2014. Prior to this date, all fluorescent lamp ballasts shall be tested using the provisions of Appendix Q. 1. Definitions 1.1...

Fluorescent lamp unit with magnetic field generating means

DOEpatents

Grossman, Mark W.; George, William A.

1989-01-01

A fluorescent lamp unit having a magnetic field generating means for improving the performance of the fluorescent lamp is disclosed. In a preferred embodiment the fluorescent lamp comprises four longitudinally extending leg portions disposed in substantially quadrangular columnar array and joined by three generally U-shaped portions disposed in different planes. In another embodiment of the invention the magnetic field generating means comprises a plurality of permanent magnets secured together to form a single columnar structure disposed within a centrally located region defined by the shape of lamp envelope.

Fluorescent lamp unit with magnetic field generating means

DOEpatents

Grossman, M.W.; George, W.A.

1989-08-08

A fluorescent lamp unit having a magnetic field generating means for improving the performance of the fluorescent lamp is disclosed. In a preferred embodiment the fluorescent lamp comprises four longitudinally extending leg portions disposed in substantially quadrangular columnar array and joined by three generally U-shaped portions disposed in different planes. In another embodiment of the invention the magnetic field generating means comprises a plurality of permanent magnets secured together to form a single columnar structure disposed within a centrally located region defined by the shape of lamp envelope. 4 figs.

A comparative study of fluorescent and LED lighting in industrial facilities

NASA Astrophysics Data System (ADS)

Perdahci PhD, C.; Akin BSc, H. C.; Cekic Msc, O.

2018-05-01

Industrial facilities have always been in search for reducing outgoings and minimizing energy consumption. Rapid developments in lighting technology require more energy efficient solutions not only for industries but also for many sectors and for households. Addition of solid-state technology has brought LED lamps into play and with LED lamp usage, efficacy level has reached its current values. Lighting systems which uses fluorescent and LED lamps have become the prior choice for many industrial facilities. This paper presents a comparative study about fluorescent and LED based indoor lighting systems for a warehouse building in an industrial facility in terms of lighting distribution values, colour rendering, power consumption, energy efficiency and visual comfort. Both scenarios have been modelled and simulated by using Relux and photometric data for the luminaires have been gathered by conducting tests and measurements in an accredited laboratory.

Study on excitation and fluorescence spectrums of Japanese citruses to construct machine vision systems for acquiring fluorescent images

NASA Astrophysics Data System (ADS)

Momin, Md. Abdul; Kondo, Naoshi; Kuramoto, Makoto; Ogawa, Yuichi; Shigi, Tomoo

2011-06-01

Research was conducted to acquire knowledge of the ultraviolet and visible spectrums from 300 -800 nm of some common varieties of Japanese citrus, to investigate the best wave-lengths for fluorescence excitation and the resulting fluorescence wave-lengths and to provide a scientific background for the best quality fluorescent imaging technique for detecting surface defects of citrus. A Hitachi U-4000 PC-based microprocessor controlled spectrophotometer was used to measure the absorption spectrum and a Hitachi F-4500 spectrophotometer was used for the fluorescence and excitation spectrums. We analyzed the spectrums and the selected varieties of citrus were categorized into four groups of known fluorescence level, namely strong, medium, weak and no fluorescence.The level of fluorescence of each variety was also examined by using machine vision system. We found that around 340-380 nm LEDs or UV lamps are appropriate as lighting devices for acquiring the best quality fluorescent image of the citrus varieties to examine their fluorescence intensity. Therefore an image acquisition device was constructed with three different lighting panels with UV LED at peak 365 nm, Blacklight blue lamps (BLB) peak at 350 nm and UV-B lamps at peak 306 nm. The results from fluorescent images also revealed that the findings of the measured spectrums worked properly and can be used for practical applications such as for detecting rotten, injured or damaged parts of a wide variety of citrus.

DOE CALiPER Program, Report 21.2: Linear (T8) LED Lamp Performance in Five Types of Recessed Troffers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, Naomi J.; Perrin, Tess E.; Royer, Michael P.

2014-05-20

Although lensed troffers are numerous, there are many other types of optical systems as well. This report looked at the performance of three linear (T8) LED lamps chosen primarily based on their luminous intensity distributions (narrow, medium, and wide beam angles) as well as a benchmark fluorescent lamp in five different troffer types. Also included are the results of a subjective evaluation. Results show that linear (T8) LED lamps can improve luminaire efficiency in K12-lensed and parabolic-louvered troffers, effect little change in volumetric and high-performance diffuse-lensed type luminaires, but reduce efficiency in recessed indirect troffers. These changes can be accompaniedmore » by visual appearance and visual comfort consequences, especially when LED lamps with clear lenses and narrow distributions are installed. Linear (T8) LED lamps with diffuse apertures exhibited wider beam angles, performed more similarly to fluorescent lamps, and received better ratings from observers. Guidance is provided on which luminaires are the best candidates for retrofitting with linear (T8) LED lamps.« less

10 CFR Appendix Q1 to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2012 CFR

2012-01-01

... of Fluorescent Lamp Ballasts Q1 Appendix Q1 to Subpart B of Part 430 Energy DEPARTMENT OF ENERGY... of Fluorescent Lamp Ballasts 1. Definitions 1.1. AC control signal means an alternating current (AC... functions. 1.3. Cathode heating refers to power delivered to the lamp by the ballast for the purpose of...

76 FR 65633 - RIN 1904-AC43

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-24

... Conservation Program: Framework Document for General Service Fluorescent Lamps and Incandescent Reflector Lamps... public meeting and availability of the framework document on general service fluorescent lamps and incandescent reflector lamps energy conservation standards in the Federal Register. This notice announces that...

[Atomic/ionic fluorescence in microwave plasma torch discharge with excitation of high current and microsecond pulsed hollow cathode lamp: Ca atomic/ionic fluorescence spectrometry].

PubMed

Gong, Zhen-bin; Liang, Feng; Yang, Peng-yuan; Jin, Qin-han; Huang, Ben-li

2002-02-01

A system of atomic and ionic fluorescence spectrometry in microwave plasma torch (MPT) discharge excited by high current microsecond pulsed hollow cathode lamp (HCMP HCL) has been developed. The operation conditions for Ca atomic and ionic fluorescence spectrometry have been optimized. Compared with atomic fluorescence spectrometry (AFS) in argon microwave induced plasma (MIP) and MPT with the excitation of direct current and conventional pulsed HCL, the system with HCMP HCL excitation can improve AFS and ionic fluorescence spectrometry (IFS) detection limits in MPT atomizer and ionizer. Detection limits (3 sigma) with HCMP HCL-MPT-AFS/IFS are 10.1 ng.mL-1 for Ca I 422.7 nm, 14.6 ng.mL-1 for Ca II 393.4 nm, and 37.4 ng.mL-1 for Ca II 396.8 nm, respectively.

An ion quencher operated lamp for multiplexed fluorescent bioassays.

PubMed

Qing, Taiping; Sun, Huanhuan; He, Xiaoxiao; Huang, Xiaoqin; He, Dinggeng; Bu, Hongchang; Qiao, Zhenzhen; Wang, Kemin

2018-02-01

A novel and adjustable lamp based on competitive interaction among dsDNA-SYBR Green I (SGI), ion quencher, and analyte was designed for bioanalysis. The "filament" and switch of the lamp could be customized by employing different dsDNA and ion quencher. The poly(AT/TA) dsDNA was successfully screened as the most effective filament of the lamp. Two common ions, Hg 2+ and Fe 3+ , were selected as the model switch, and the corresponding ligand molecules cysteine (Cys) and pyrophosphate ions (PPi) were selected as the targets. When the fluorescence-quenched dsDNA/SGI-ion complex was introduced into a target-containing system, ions could be bound by competitive molecules and separate from the complex, thereby lighting the lamp. However, no light was observed if the biomolecule could not snatch the metal ions from the complex. Under the optimal conditions, sensitive and selective detection of Cys and PPi was achieved by the lamp, with practical applications in fetal bovine serum and human urine. This ion quencher regulated lamp for fluorescent bioassays is simple in design, fast in operation, and is more convenient than other methods. Significantly, as many molecules could form stable complexes with metal ions selectively, this ion quencher operated lamp has potential for the detection of a wide spectrum of analytes. Graphical abstract A novel and adjustable lamp on the basis of competitive interaction among dsDNA-SYBR Green I, ions quencher and analyte was designed for bioanalysis. The filament and switch of lamp could be customized by employing different dsDNA and ions quencher.

10 CFR Appendix W to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Medium Base Compact Fluorescent Lamps

Code of Federal Regulations, 2013 CFR

2013-01-01

... “ENERGY STAR Program Requirements for [Compact Fluorescent Lamps] CFLs,” Version dated August 9, 2001... DOE's “ENERGY STAR Program Requirements for [Compact Fluorescent Lamps] CFLs,” Version dated August 9...

75 FR 36119 - In the Matter of Certain Cold Cathode Fluorescent Lamp (“CCFL”) Inverter Circuits and Products...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-24

... Cathode Fluorescent Lamp (``CCFL'') Inverter Circuits and Products Containing the Same; Notice of... States after importation of certain cold cathode fluorescent lamp inverter circuits and products..., and the sale within the United States after importation of CCFL inverter circuits and products...

Mercury mass measurement in fluorescent lamps via neutron activation analysis

NASA Astrophysics Data System (ADS)

Viererbl, L.; Vinš, M.; Lahodová, Z.; Fuksa, A.; Kučera, J.; Koleška, M.; Voljanskij, A.

2015-11-01

Mercury is an essential component of fluorescent lamps. Not all fluorescent lamps are recycled, resulting in contamination of the environment with toxic mercury, making measurement of the mercury mass used in fluorescent lamps important. Mercury mass measurement of lamps via instrumental neutron activation analysis (NAA) was tested under various conditions in the LVR-15 research reactor. Fluorescent lamps were irradiated in different positions in vertical irradiation channels and a horizontal channel in neutron fields with total fluence rates from 3×108 cm-2 s-1 to 1014 cm-2 s-1. The 202Hg(n,γ)203Hg nuclear reaction was used for mercury mass evaluation. Activities of 203Hg and others induced radionuclides were measured via gamma spectrometry with an HPGe detector at various times after irradiation. Standards containing an Hg2Cl2 compound were used to determine mercury mass. Problems arise from the presence of elements with a large effective cross section in luminescent material (europium, antimony and gadolinium) and glass (boron). The paper describes optimization of the NAA procedure in the LVR-15 research reactor with particular attention to influence of neutron self-absorption in fluorescent lamps.

Characterization and recovery of mercury from spent fluorescent lamps.

PubMed

Jang, Min; Hong, Seung Mo; Park, Jae K

2005-01-01

Fluorescent lamps rely on mercury as the source of ultraviolet radiation for the production of visible light. Partitioning of mercury among vapor phase, loose phosphor powders produced during breaking and washing steps, glass matrices, phosphor powders attached on the glass and aluminum end caps was examined from simulated laboratory lamp recycling tests for different types of spent and new fluorescent lamps. Mercury concentrations in lamp glasses taken from commercial lamp recyclers were also analyzed for comparison with the simulated results of spent and new lamps of different types. The mercury content of the glass from spent lamps was highly variable depending on the lamp type and manufacturer; the median values of the mercury concentration in glasses for spent 26- (T8) and 38-mm (T12) diameter fluorescent lamps were approximately 30 and 45 microg/g, respectively. The average mercury concentration of samples taken from recycler A was 29.6 microg/g, which was about 64% of median value measured from the spent T12 lamps. Over 94% of total mercury in lamps remained either as a component of phosphor powders attached inside the lamp or in glass matrices. New T12 lamps had a higher partitioning percentage of elemental mercury in the vapor phase (0.17%) than spent T12 lamps (0.04%), while spent lamps had higher partitioning percentages of mercury resided on end-caps and phosphor powders detached from the breaking and washing steps. The TCLP values of simulated all lamp-glasses and samples obtained from recyclers were higher than the limit of LDR standard (0.025 mg/L). After investigating acid treatment and high temperature treatment as mercury reclamation techniques, it was found that heating provided the most effective mercury capture. Although the initial mercury concentrations of individual sample were different, the mercury concentrations after 1 h exposure at 100 degrees C were below 4 mug/g for all samples (i.e., <1% remaining). Therefore, it is recommended that heating be used for recovering mercury from spent fluorescent lamps.

Compact fluorescent lamp using horizontal and vertical insulating septums and convective venting geometry

DOEpatents

Siminovitch, Michael

1998-01-01

A novel design for a compact fluorescent lamp, including a lamp geometry which will increase light output and efficacy of the lamp in a base down operating position by providing horizontal and vertical insulating septums positioned in the ballast compartment of the lamp to provide a cooler coldspot. Selective convective venting provides additional cooling of the ballast compartment.

CALiPER Report 21.2. Linear (T8) LED Lamp Performance in Five Types of Recessed Troffers

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2014-05-01

Although lensed troffers are numerous, there are many other types of optical systems as well. This report looks at the performance of three linear (T8) LED lamps—chosen primarily based on their luminous intensity distributions (narrow, medium, and wide beam angles)—as well as a benchmark fluorescent lamp in five different troffer types. Also included are the results of a subjective evaluation. Results show that linear (T8) LED lamps can improve luminaire efficiency in K12-lensed and parabolic-louvered troffers, effect little change in volumetric and high-performance diffuse-lensed type luminaires, but reduce efficiency in recessed indirect troffers. These changes can be accompanied by visualmore » appearance and visual comfort consequences, especially when LED lamps with clear lenses and narrow distributions are installed. Linear (T8) LED lamps with diffuse apertures exhibited wider beam angles, performed more similarly to fluorescent lamps, and received better ratings from observers. Guidance is provided on which luminaires are the best candidates for retrofitting with linear (T8) LED lamps.« less

Heat transfer assembly for a fluorescent lamp and fixture

DOEpatents

Siminovitch, Michael J.; Rubenstein, Francis M.; Whitman, Richard E.

1992-01-01

In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure.

Compact fluorescent lamp using horizontal and vertical insulating septums and convective venting geometry

DOEpatents

Siminovitch, M.

1998-02-10

A novel design is described for a compact fluorescent lamp, including a lamp geometry which will increase light output and efficacy of the lamp in a base down operating position by providing horizontal and vertical insulating septums positioned in the ballast compartment of the lamp to provide a cooler coldspot. Selective convective venting provides additional cooling of the ballast compartment. 9 figs.

Max Tech and Beyond: Fluorescent Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scholand, Michael

2012-04-01

Fluorescent lamps are the most widely used artificial light source today, responsible for approximately 70% of the lumens delivered to our living spaces globally. The technology was originally commercialized in the 1930's, and manufacturers have been steadily improving the efficacy of these lamps over the years through modifications to the phosphors, cathodes, fill-gas, operating frequency, tube diameter and other design attributes. The most efficient commercially available fluorescent lamp is the 25 Watt T5 lamp. This lamp operates at 114-116 lumens per watt while also providing good color rendering and more than 20,000 hours of operating life. Industry experts interviewed indicatedmore » that while this lamp is the most efficient in the market today, there is still a further 10 to 14% of potential improvements that may be introduced to the market over the next 2 to 5 years. These improvements include further developments in phosphors, fill-gas, cathode coatings and ultraviolet (UV) reflective glass coatings. The commercialization of these technology improvements will combine to bring about efficacy improvements that will push the technology up to a maximum 125 to 130 lumens per watt. One critical issue raised by researchers that may present a barrier to the realization of these improvements is the fact that technology investment in fluorescent lamps is being reduced in order to prioritize research into light emitting diodes (LEDs) and ceramic metal halide high intensity discharge (HID) lamps. Thus, it is uncertain whether these potential efficacy improvements will be developed, patented and commercialized. The emphasis for premium efficacy will continue to focus on T5 lamps, which are expected to continue to be marketed along with the T8 lamp. Industry experts highlighted the fact that an advantage of the T5 lamp is the fact that it is 40% smaller and yet provides an equivalent lumen output to that of a T8 or T12 lamp. Due to its smaller form factor, the T5 lamp contains less material (i.e., glass, fill gas and phosphor), and has a higher luminance, enabling fixtures to take advantage of the smaller lamp size to improve the optics and provide more efficient overall system illuminance. In addition to offering the market a high-quality efficacious light source, another strong value proposition of fluorescent lighting is its long operating life. In today's market, one manufacturer is offering fluorescent lamps that have a rated life of 79,000 hours - which represents 18 years of service at 12 hours per day, 365 days per year. These lamps, operated using a long-life ballast specified by the manufacturer, take advantage of improvements in cathode coatings, fill gas chemistry and pressure to extend service life by a factor of four over conventional fluorescent lamps. It should be noted that this service life is also longer (approximately twice as long) as today's high-quality LED products. The fluorescent market is currently focused on the T5 and T8 lamp diameters, and it is not expected that other diameters would be introduced. Although T8 is a more optimal diameter from an efficacy perspective, the premium efficiency and optimization effort has been focused on T5 lamps because they are 40% smaller than T8, and are designed to operate at a higher temperature using high-frequency electronic ballasts. The T5 lamp offers savings in terms of materials, packaging and shipping, as well as smaller fixtures with improved optical performance. Manufacturers are actively researching improvements in four critical areas that are expected to yield additional efficacy improvements of approximately 10 to 14 percent over the next five years, ultimately achieving approximately 130 lumens per watt by 2015. The active areas of research where these improvements are anticipated include: (1) Improved phosphors which continue to be developed and patented, enabling higher efficacies as well as better color rendering and lumen maintenance; (2) Enhanced fill gas - adjusting proportions of argon, krypton, neon and xenon to optimize performance, while also minimizing the mercury dose; (3) Improved cathode coatings to enhance electron emissivity and extend lamp life; and (4) UV-reflective glass coatings deposited between the layer of phosphor and the glass tube, to reflect any UV light back into the phosphor layer for down-conversion.« less

10 CFR 429.35 - Bare or covered (no reflector) medium base compact fluorescent lamps.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 3 2012-01-01 2012-01-01 false Bare or covered (no reflector) medium base compact....35 Bare or covered (no reflector) medium base compact fluorescent lamps. (a) Sampling plan for... reflector) medium base compact fluorescent lamps; and (2) For each basic model of bare or covered (no...

10 CFR 429.35 - Bare or covered (no reflector) medium base compact fluorescent lamps.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 3 2014-01-01 2014-01-01 false Bare or covered (no reflector) medium base compact....35 Bare or covered (no reflector) medium base compact fluorescent lamps. (a) Sampling plan for... reflector) medium base compact fluorescent lamps; and (2) For each basic model of bare or covered (no...

10 CFR 429.35 - Bare or covered (no reflector) medium base compact fluorescent lamps.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 3 2013-01-01 2013-01-01 false Bare or covered (no reflector) medium base compact....35 Bare or covered (no reflector) medium base compact fluorescent lamps. (a) Sampling plan for... reflector) medium base compact fluorescent lamps; and (2) For each basic model of bare or covered (no...

Energy Efficiency Comparison between Compact Fluorescent Lamp and Common Light Bulb

ERIC Educational Resources Information Center

Tanushevsk, Atanas; Rendevski, Stojan

2016-01-01

For acquainting the students of applied physics and students of teaching physics with the concept of energy efficiency, electrical and spectral characteristics of two widely used lamps--integrated fluorescence lamp and common light bulb have been investigated. Characterization of the lamps has been done by measuring the spectral irradiance and…

Light Sources and Ballast Circuits

NASA Astrophysics Data System (ADS)

Yorifuji, Takashi; Sakai, Makoto; Yasuda, Takeo; Maehara, Akiyoshi; Okada, Atsunori; Gouriki, Takeshi; Mannami, Tomoaki

According to the machinery statistics by Ministry of Economy, Trade and Industry (METI), the total of domestic light bulb production in 2006 was 1,101 million (88.5% year-on-year). Production for general purpose illumination light bulbs and halogen light bulbs accounted for 122 million (99.2% y/y) and 45 million (96.3% y/y), respectively. The total of fluorescent lamp production was 988 million (114.9%) and the production of general purpose fluorescent lamps excluding backlights accounted for 367 million (101.7% y/y). Further, HID lamp production was 10 million (106.3% y/y). What is noteworthy regarding such lamp production is that, similar to the previous year, the sales volume (amount) of lamps for general illumination exceeded 100% against the previous year, indicating a steady shift to high value added products. Major lighting exhibitions in 2006 included the Light + Building Trade Fair held in Frankfurt in April and the Light Fair International 2006 held in Las Vegas, U.S.A. in May, both of which demonstrated signs of acceleration toward energy saving, high efficiency and resource saving. As for incandescent lamps, products filled with larger atomic weight gases aiming at higher efficiency/longer life are becoming the mainstream. As for new technologies, it was experimentally demonstrated that infrared radiation can be suppressed by processing micro cavities to metal plates made of tungsten, tantalum, etc. For fluorescent lamps, straight and circular fluorescent lamps achieving a longer life/higher luminous flux maintenance factor continued to be widely developed/launched again this year. For compact fluorescent lamps, energy saving/high efficiency products, multifunctional type products combined with LED and new shaped products were launched. As to HID lamps, ceramic metal halide lamps with high efficiency, improved color rendering, longer life and higher luminous flux maintenance factor were commercialized one after another. Numerous studies and analyses, on discharge models were reported. Further, studies on ultra high-pressure mercury lamps as light sources for projectors are becoming the mainstream of HID lamp related researches. For high-pressure sodium lamps, many studies on plant growing and pest control utilizing low insect attracting aspects were also reported in 2006. Additionally, for discharge lamps, the minimum sustaining electric power for arc tubes employed in electrode-less compact fluorescent lamps was investigated. For Hg-free rare-gas fluorescent lamps, a luminance of 10,000cd/m2 was attained by a 1 meter-long external duplex spiral electrode prototype using Xe/Ne barrier discharge. As to startup circuits, the commercialization of energy saving and high value added products mainly associated with fluorescent lamps and HID lamps are becoming common. Further, the miniaturization of startup circuits for self electronic-ballasted lamps has advanced. Speaking of the overall light sources and startup circuits in 2006 and with the enforcement of RoHS in Europe in July, the momentum toward hazardous substance-free and energy saving initiatives has been enhanced from the perspective of protecting the global environment. It is anticipated that similar restrictions will be globally enforced in the future.

A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis.

PubMed

Xu, Jing; Xiong, Yan; Chen, Shiheng; Guan, Yafeng

2008-07-15

A light-emitting diode-induced fluorescence detector (LED-FD) for capillary electrophoresis was constructed and evaluated. A lamp LED with an enhanced emission spectrum and a band pass filter was used as the excitation light source. Refractive index matching fluid (RIMF) was used in the detection cell to reduce scattering light and the noise level. The limit of detection (LOD) for fluorescein was 1.5 nM (SNR=3). The system exhibited linear responses in the range of 1 x 10(-8) to 5 x 10(-6)M (R=0.999). Application of the lamp LED-FD for the analysis of FITC-labeled ephedra herb extract by capillary electrophoresis was demonstrated.

Heat transfer assembly for a fluorescent lamp and fixture

DOEpatents

Siminovitch, M.J.; Rubenstein, F.M.; Whitman, R.E.

1992-12-29

In a lighting fixture including a lamp and a housing, a heat transfer structure is disclosed for reducing the minimum lamp wall temperature of a fluorescent light bulb. The heat transfer structure, constructed of thermally conductive material, extends from inside the housing to outside the housing, transferring heat energy generated from a fluorescent light bulb to outside the housing where the heat energy is dissipated to the ambient air outside the housing. Also disclosed is a method for reducing minimum lamp wall temperatures. Further disclosed is an improved lighting fixture including a lamp, a housing and the aforementioned heat transfer structure. 11 figs.

Frequency Domain Fluorimetry Using a Mercury Vapor Lamp

DTIC Science & Technology

2009-04-07

independence from light scatter and excitation/emission intensity variations in order to extract the sample’s fluorescent lifetime. Mercury vapor lamps ...the modulation amplitude of the lamp , An, via: max 0 1 ( ) sin(2 ) n fluorescence n n n I t B nf tπ θ = ∝ +∑ (8... lamp is estimated by assuming the lamp is emitting as a point source of uniform intensity into the lower hemisphere and has a reflector collecting

Fluorescent discharge lamp

NASA Technical Reports Server (NTRS)

Mukai, E.; Otsuka, H.; Nomi, K.; Honmo, I.

1982-01-01

A rapidly illuminating fluorescent lamp 1,200 mm long and 32.5 mm in diameter with an interior conducting strip which is compatible with conventional fixtures and ballasts is described. The fluorescent lamp is composed of a linear glass tube, electrodes sealed at both ends, mercury and raregas sealed in the glass tube, a fluorescent substance clad on the inner walls of the glass tube, and a clad conducting strip extending the entire length of the glass tube in the axial direction on the inner surface of the tube.

Recovery of yttrium from cathode ray tubes and lamps’ fluorescent powders: experimental results and economic simulation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Innocenzi, V., E-mail: valentina.innocenzi1@univaq.it; De Michelis, I.; Ferella, F.

2013-11-15

Highlights: • Fluorescent powder of lamps. • Fluorescent powder of cathode ray rubes. • Recovery of yttrium from fluorescent powders. • Economic simulation for the processes to recover yttrium from WEEE. - Abstract: In this paper, yttrium recovery from fluorescent powder of lamps and cathode ray tubes (CRTs) is described. The process for treating these materials includes the following: (a) acid leaching, (b) purification of the leach liquors using sodium hydroxide and sodium sulfide, (c) precipitation of yttrium using oxalic acid, and (d) calcinations of oxalates for production of yttrium oxides. Experimental results have shown that process conditions necessary tomore » purify the solutions and recover yttrium strongly depend on composition of the leach liquor, in other words, whether the powder comes from treatment of CRTs or lamp. In the optimal experimental conditions, the recoveries of yttrium oxide are about 95%, 55%, and 65% for CRT, lamps, and CRT/lamp mixture (called MIX) powders, respectively. The lower yields obtained during treatments of MIX and lamp powders are probably due to the co-precipitation of yttrium together with other metals contained in the lamps powder only. Yttrium loss can be reduced to minimum changing the experimental conditions with respect to the case of the CRT process. In any case, the purity of final products from CRT, lamps, and MIX is greater than 95%. Moreover, the possibility to treat simultaneously both CRT and lamp powders is very important and interesting from an industrial point of view since it could be possible to run a single plant treating fluorescent powder coming from two different electronic wastes.« less

CALiPER Report 21.3: Cost-Effectiveness of Linear (T8) LED Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, Naomi J.; Perrin, Tess E.; Royer, Michael P.

2014-05-27

Meeting performance expectations is important for driving adoption of linear LED lamps, but cost-effectiveness may be an overriding factor in many cases. Linear LED lamps cost more initially than fluorescent lamps, but energy and maintenance savings may mean that the life-cycle cost is lower. This report details a series of life-cycle cost simulations that compared a two-lamp troffer using LED lamps (38 W total power draw) or fluorescent lamps (51 W total power draw) over a 10-year study period. Variables included LED system cost ($40, $80, or $120), annual operating hours (2,000 hours or 4,000 hours), LED installation time (15more » minutes or 30 minutes), and melded electricity rate ($0.06/kWh, $0.12/kWh, $0.18/kWh, or $0.24/kWh). A full factorial of simulations allows users to interpolate between these values to aid in making rough estimates of economic feasibility for their own projects. In general, while their initial cost premium remains high, linear LED lamps are more likely to be cost-effective when electric utility rates are higher than average and hours of operation are long, and if their installation time is shorter.« less

CALiPER Report 21.3. Cost Effectiveness of Linear (T8) LED Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2014-05-01

Meeting performance expectations is important for driving adoption of linear LED lamps, but cost-effectiveness may be an overriding factor in many cases. Linear LED lamps cost more initially than fluorescent lamps, but energy and maintenance savings may mean that the life-cycle cost is lower. This report details a series of life-cycle cost simulations that compared a two-lamp troffer using LED lamps (38 W total power draw) or fluorescent lamps (51 W total power draw) over a 10-year study period. Variables included LED system cost ($40, $80, or $120), annual operating hours (2,000 hours or 4,000 hours), LED installation time (15more » minutes or 30 minutes), and melded electricity rate ($0.06/kWh, $0.12/kWh, $0.18/kWh, or $0.24/kWh). A full factorial of simulations allows users to interpolate between these values to aid in making rough estimates of economic feasibility for their own projects. In general, while their initial cost premium remains high, linear LED lamps are more likely to be cost-effective when electric utility rates are higher than average and hours of operation are long, and if their installation time is shorter.« less

[Atomic/ionic fluorescence in microwave plasma torch discharge excited by high current microsecond pulsed hollow cathode lamp-europium atomic/ionic fluorescence spectrometry].

PubMed

Gong, Z; Liang, F; Yang, P; Jin, Q; Huang, B

1999-06-01

Eu atomic and ionic fluorescence spectrometry in microwave plasma torch discharge excited by high current microsecond pulsed hollow cathode lamp (HCMP HCL-MPT AFS/IFS) was studied. Operating conditions were optimized. The best detection limits for AFS and IFS obtained with a desolvated ultrasonic nebulization system were 42.0 ng/mL for Eu I 462.7 nm and 21.8 ng/mL for Eu II 381.97 nm, respectively, both were better than those given by the instruction manual of a Baird ICP AFS-2000 spectrometer using pneumatic concentric nebulizer with desolvation for AFS, but were significantly higher than those obtained by using the Baird spectrometer with a mini-monochromator and a ultrasonic nebulzer system.

Fluorescent lamp with static magnetic field generating means

DOEpatents

Moskowitz, Philip E.; Maya, Jakob

1987-01-01

A fluorescent lamp wherein magnetic field generating means (e.g., permanent magnets) are utilized to generate a static magnetic field across the respective electrode structures of the lamp such that maximum field strength is located at the electrode's filament. An increase in efficacy during operation has been observed.

Fluorescent lamp with static magnetic field generating means

DOEpatents

Moskowitz, P.E.; Maya, J.

1987-09-08

A fluorescent lamp wherein magnetic field generating means (e.g., permanent magnets) are utilized to generate a static magnetic field across the respective electrode structures of the lamp such that maximum field strength is located at the electrode's filament. An increase in efficacy during operation has been observed. 2 figs.

Compact whole-body fluorescent imaging of nude mice bearing EGFP expressing tumor

NASA Astrophysics Data System (ADS)

Chen, Yanping; Xiong, Tao; Chu, Jun; Yu, Li; Zeng, Shaoqun; Luo, Qingming

2005-01-01

Issue of tumor has been a hotspot of current medicine. It is important for tumor research to detect tumors bearing in animal models easily, fast, repetitively and noninvasivly. Many researchers have paid their increasing interests on the detecting. Some contrast agents, such as green fluorescent protein (GFP) and Discosoma red fluorescent protein (Dsred) were applied to enhance image quality. Three main kinds of imaging scheme were adopted to visualize fluorescent protein expressing tumors in vivo. These schemes based on fluorescence stereo microscope, cooled charge-coupled-device (CCD) or camera as imaging set, and laser or mercury lamp as excitation light source. Fluorescence stereo microscope, laser and cooled CCD are expensive to many institutes. The authors set up an inexpensive compact whole-body fluorescent imaging tool, which consisted of a Kodak digital camera (model DC290), fluorescence filters(B and G2;HB Optical, Shenyang, Liaoning, P.R. China) and a mercury 50-W lamp power supply (U-LH50HG;Olympus Optical, Japan) as excitation light source. The EGFP was excited directly by mercury lamp with D455/70 nm band-pass filter and fluorescence was recorded by digital camera with 520nm long-pass filter. By this easy operation tool, the authors imaged, in real time, fluorescent tumors growing in live mice. The imaging system is external and noninvasive. For half a year our experiments suggested the imaging scheme was feasible. Whole-body fluorescence optical imaging for fluorescent expressing tumors in nude mouse is an ideal tool for antitumor, antimetastatic, and antiangiogenesis drug screening.

The photoluminescence of a fluorescent lamp: didactic experiments on the exponential decay

NASA Astrophysics Data System (ADS)

Onorato, Pasquale; Gratton, Luigi; Malgieri, Massimiliano; Oss, Stefano

2017-01-01

The lifetimes of the photoluminescent compounds contained in the coating of fluorescent compact lamps are usually measured using specialised instruments, including pulsed lasers and/or spectrofluorometers. Here we discuss how some low cost apparatuses, based on the use of either sensors for the educational lab or commercial digital photo cameras, can be employed to the same aim. The experiments do not require that luminescent phosphors are hazardously extracted from the compact fluorescent lamp, that also contains mercury. We obtain lifetime measurements for specific fluorescent elements of the bulb coating, in good agreement with the known values. We also address the physical mechanisms on which fluorescence lamps are based in a simplified way, suitable for undergraduate students; and we discuss in detail the physics of the lamp switch-off by analysing the time dependent spectrum, measured through a commercial fiber-optic spectrometer. Since the experiment is not hazardous in any way, requires a simple setup up with instruments which are commonly found in educational labs, and focuses on the typical features of the exponential decay, it is suitable for being performed in the undergraduate laboratory.

CALiPER Application Summary Report 21. Linear (T8) LED Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2014-03-01

This report focuses on the bare lamp performance of 31 linear LED lamps intended as an alternative to T8 fluorescent lamps. Data obtained in accordance with IES LM-79-08 indicated that the mean efficacy is similar to that of fluorescent lamps, but that lumen output is often much lower. This presents a situation where something must change in order for energy savings and equivalent illumination levels to be achieved simultaneously. In this case, the luminous intensity distribution of all the tested lamps was directional or semi-directional, rather than omnidirectional. Also discussed in this report are several issues related to the electricalmore » configuration of the lamps, such as the required socket types and power feed location. While no configuration is necessarily better, the multitude of options can make specifying and installing linear LED lamps more difficult, with the potential for safety issues. Similarly, the variety of color and power quality attributes adds a layer of complexity to the specification process. Many products offered good or excellent quality attributes, but some did not and thus could be perceived as inferior to fluorescent lamps in some installations.« less

21 CFR 866.2600 - Wood's fluorescent lamp.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Wood's fluorescent lamp. 866.2600 Section 866.2600 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2600 Wood's fluorescent...

21 CFR 866.2600 - Wood's fluorescent lamp.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Wood's fluorescent lamp. 866.2600 Section 866.2600 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2600 Wood's fluorescent...

21 CFR 866.2600 - Wood's fluorescent lamp.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Wood's fluorescent lamp. 866.2600 Section 866.2600 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2600 Wood's fluorescent...

21 CFR 866.2600 - Wood's fluorescent lamp.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Wood's fluorescent lamp. 866.2600 Section 866.2600 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2600 Wood's fluorescent...

21 CFR 866.2600 - Wood's fluorescent lamp.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Wood's fluorescent lamp. 866.2600 Section 866.2600 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Microbiology Devices § 866.2600 Wood's fluorescent...

Comparison of loop-mediated isothermal amplification assay and smear microscopy with culture for the diagnostic accuracy of tuberculosis.

PubMed

Gelaw, Baye; Shiferaw, Yitayal; Alemayehu, Marta; Bashaw, Abate Assefa

2017-01-17

Tuberculosis (TB) caused by Mycobacterium tuberculosis is one of the leading causes of death from infectious diseases worldwide. Sputum smear microscopy remains the most widely available pulmonary TB diagnostic tool particularly in resource limited settings. A highly sensitive diagnostic with minimal infrastructure, cost and training is required. Hence, we assessed the diagnostic performance of Loop-mediated isothermal amplification (LAMP) assay in detecting M.tuberculosis infection in sputum sample compared to LED fluorescent smear microscopy and culture. A cross-sectional study was conducted at the University of Gondar Hospital from June 01, 2015 to August 30, 2015. Pulmonary TB diagnosis using sputum LED fluorescence smear microscopy, TB-LAMP assay and culture were done. A descriptive analysis was used to determine demographic characteristics of the study participants. Analysis of sensitivity and specificity for smear microscopy and TB-LAMP compared with culture as a reference test was performed. Cohen's kappa was calculated as a measure of agreement between the tests. A total of 78 pulmonary presumptive TB patients sputum sample were analyzed. The overall sensitivity and specificity of LAMP were 75 and 98%, respectively. Among smear negative sputum samples, 33.3% sensitivity and 100% specificity of LAMP were observed. Smear microscopy showed 78.6% sensitivity and 98% specificity. LAMP and smear in series had sensitivity of 67.8% and specificity of 100%. LAMP and smear in parallel had sensitivity of 85.7% and specificity of 96%. The agreement between LAMP and fluorescent smear microscopy tests was very good (κ = 0.83, P-value ≤0.0001). TB-LAMP showed similar specificity but a slightly lower sensitivity with LED fluorescence microscopy. The specificity of LAMP and smear microscopy in series was high. The sensitivity of LAMP was insufficient for smear negative sputum samples.

Light Sources and Lighting Circuits

NASA Astrophysics Data System (ADS)

Honda, Hisashi; Suwa, Takumi; Yasuda, Takeo; Ohtani, Yoshihiko; Maehara, Akiyoshi; Okada, Atsunori; Komatsu, Naoki; Mannami, Tomoaki

According to the Machinery Statistics of the Ministry of Economy, Trade and Industry, the production of incandescent lamps in Japan in 2007 was 990 million units (90.0% of the previous year's total), in which the production of incandescent lamps for general lighting was 110 million units (90.0% of the previous year's total) and of tungsten-halogen lamps was 44 million units (96.6% of the previous year's total). The production of fluorescent lamps was 927 million units (93.9% of the previous year's total), in which general fluorescent lamps, excluding those for LCD back lighting, was 320 million units (87.2% of the previous year's total). Also, the production of HID lamps was 10 million units (101.5% of the previous year's total). On the other hand, when the numbers of sales are compared with the sales of the previous year, incandescent lamps for general use was 99.8%, tungsten-halogen lamps was 96.9%, fluorescent lamps was 95.9%, and HID lamps was 98.9%. Self-ballasted fluorescent lamps alone showed an increase in sales as strong as 29 million units, or 121.7% of the previous year's sales. It is considered that the switchover of incandescent lamps to HID lamps was promoted for energy conservation and carbon dioxide reduction with the problem of global warming in the background. In regard to exhibitions, Lighting Fair 2007 was held in Tokyo in March, and LIGHTFAIR INTERNATIONAL 2007 was held in New York in May. Regarding academic conferences, LS:11 (the 11th International Symposium on the Science & Technology of Light Sources) was held in Shanghai in May, and the First International Conference on White LEDs and Solid State Lighting was held in Tokyo in November. Both conferences suggested that there are strong needs and concerns now about energy conservation, saving natural resources, and restrictions of hazardous materials. In regard to incandescent lamps, the development of products aiming at higher efficacy, electric power savings, and longer life was advanced by means of using filler gas with a higher atomic weight. Regarding fluorescent lamps, studies and developments for longer operating life and improvement in the lumen maintenance factor for the straight-type and circular-type fluorescent lamps were actively pursued. Regarding self-ballasted fluorescent lamps, the main stream of development was aimed at reducing lamp size and increasing energy conservation, and the development of new products that took advantage of these features proceeded. In regard to LED light sources, basic research and product development, including new application development, were vigorously implemented. In basic research, studies were reported, not only on efficacy improvements through optimization of the LED chips, phosphor layers, and packaging technology, but also on photometry, colorimetry, and visual psychology. In the field of application, applications were studied for general lighting sources and also for a wide range of fields, such as automotive headlights and visible light communication. Also, many academic conferences and exhibitions were held domestically and overseas, and the high level of interest suggests high expectations for this next-generation light source. Regarding HID lamps, there was much activity in research and development and in the commercialization of the ceramic metal halide lamp product, and products were commercialized with features such as higher efficiency (130 lm/W) and higher color rendering properties (R9 ≥ 90). In the high-pressure sodium lamps, there were many study reports concerning plant growth and insect pest control using its low insect-attracting characteristics. With high-pressure mercury lamps, there were many reports on reducing lamp size and increasing intensity for use as a light source for projectors.

Secondary electron emission and glow discharge properties of 12CaO·7Al2O3 electride for fluorescent lamp applications.

PubMed

Watanabe, Satoru; Watanabe, Toshinari; Ito, Kazuhiro; Miyakawa, Naomichi; Ito, Setsuro; Hosono, Hideo; Mikoshiba, Shigeo

2011-06-01

12CaO·7Al 2 O 3 electride, a sub-nanoporous compound having a work function of 2.4 eV, was examined as a candidate cathode material in fluorescent lamps. The electron emission yield was higher and the discharge voltage was lower for 12CaO·7Al 2 O 3 than for existing cathode materials such as Ni, Mo or W; therefore, the energy consumption of the fluorescent lamps can be improved using 12CaO·7Al 2 O 3 cathodes. Prototype glow-discharge lamps using 12CaO·7Al 2 O 3 were constructed and exhibited reasonable durability.

Secondary electron emission and glow discharge properties of 12CaO·7Al2O3 electride for fluorescent lamp applications

PubMed Central

Watanabe, Satoru; Watanabe, Toshinari; Ito, Kazuhiro; Miyakawa, Naomichi; Ito, Setsuro; Hosono, Hideo; Mikoshiba, Shigeo

2011-01-01

12CaO·7Al2O3 electride, a sub-nanoporous compound having a work function of 2.4 eV, was examined as a candidate cathode material in fluorescent lamps. The electron emission yield was higher and the discharge voltage was lower for 12CaO·7Al2O3 than for existing cathode materials such as Ni, Mo or W; therefore, the energy consumption of the fluorescent lamps can be improved using 12CaO·7Al2O3 cathodes. Prototype glow-discharge lamps using 12CaO·7Al2O3 were constructed and exhibited reasonable durability. PMID:27877401

Custom blending of lamp phosphors

NASA Technical Reports Server (NTRS)

Klemm, R. E.

1978-01-01

Spectral output of fluorescent lamps can be precisely adjusted by using computer-assisted analysis for custom blending lamp phosphors. With technique, spectrum of main bank of lamps is measured and stored in computer memory along with emission characteristics of commonly available phosphors. Computer then calculates ratio of green and blue intensities for each phosphor according to manufacturer's specifications and plots them as coordinates on graph. Same ratios are calculated for measured spectrum. Once proper mix is determined, it is applied as coating to fluorescent tubing.

340 nm pulsed UV LED system for europium-based time-resolved fluorescence detection of immunoassays.

PubMed

Rodenko, Olga; Fodgaard, Henrik; Tidemand-Lichtenberg, Peter; Petersen, Paul Michael; Pedersen, Christian

2016-09-19

We report on the design, development and investigation of an optical system based on UV light emitting diode (LED) excitation at 340 nm for time-resolved fluorescence detection of immunoassays. The system was tested to measure cardiac marker Troponin I with a concentration of 200 ng/L in immunoassay. The signal-to-noise ratio was comparable to state-of-the-art Xenon flash lamp based unit with equal excitation energy and without overdriving the LED. We performed a comparative study of the flash lamp and the LED based system and discussed temporal, spatial, and spectral features of the LED excitation for time-resolved fluorimetry. Optimization of the suggested key parameters of the LED promises significant increase of the signal-to-noise ratio and hence of the sensitivity of immunoassay systems.

Light-emitting diodes as a radiation source for plants

NASA Technical Reports Server (NTRS)

Bula, R. J.; Morrow, R. C.; Tibbitts, T. W.; Barta, D. J.; Ignatius, R. W.; Martin, T. S.

1991-01-01

Development of a more effective radiation source for use in plant-growing facilities would be of significant benefit for both research and commercial crop production applications. An array of light-emitting diodes (LEDs) that produce red radiation, supplemented with a photosynthetic photon flux (PPF) of 30 micromoles s-1 m-2 in the 400- to 500-nm spectral range from blue fluorescent lamps, was used effectively as a radiation source for growing plants. Growth of lettuce (Lactuca sativa L. Grand Rapids') plants maintained under the LED irradiation system at a total PPF of 325 micromoles s-1 m-2 for 21 days was equivalent to that reported in the literature for plants grown for the same time under cool-white fluorescent and incandescent radiation sources. Characteristics of the plants, such as leaf shape, color, and texture, were not different from those found with plants grown under cool-white fluorescent lamps. Estimations of the electrical energy conversion efficiency of a LED system for plant irradiation suggest that it may be as much as twice that published for fluorescent systems.

The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification (LAMP) Assay for Tuberculosis Diagnosis in Adults with Chronic Cough in Malawi.

PubMed

Nliwasa, Marriott; MacPherson, Peter; Chisala, Palesa; Kamdolozi, Mercy; Khundi, McEwen; Kaswaswa, Kruger; Mwapasa, Mphatso; Msefula, Chisomo; Sohn, Hojoon; Flach, Clare; Corbett, Elizabeth L

2016-01-01

Current tuberculosis diagnostics lack sensitivity, and are expensive. Highly accurate, rapid and cheaper diagnostic tests are required for point of care use in low resource settings with high HIV prevalence. To investigate the sensitivity and specificity, and cost of loop-mediated isothermal amplification (LAMP) assay for tuberculosis diagnosis in adults with chronic cough compared to Xpert® MTB/RIF, fluorescence smear microscopy. Between October 2013 and March 2014, consecutive adults at a primary care clinic were screened for cough, offered HIV testing and assessed for tuberculosis using LAMP, Xpert® MTB/RIF and fluorescence smear microscopy. Sensitivity and specificity (with culture as reference standard), and costs were estimated. Of 273 adults recruited, 44.3% (121/273) were HIV-positive and 19.4% (53/273) had bacteriogically confirmed tuberculosis. The sensitivity of LAMP compared to culture was 65.0% (95% CI: 48.3% to 79.4%) with 100% (95% CI: 98.0% to 100%) specificity. The sensitivity of Xpert® MTB/RIF (77.5%, 95% CI: 61.5% to 89.2%) was similar to that of LAMP, p = 0.132. The sensitivity of concentrated fluorescence smear microscopy with routine double reading (87.5%, 95% CI: 73.2% to 95.8%) was higher than that of LAMP, p = 0.020. All three tests had high specificity. The lowest cost per test of LAMP was at batch size of 14 samples (US$ 9.98); this was lower than Xpert® MTB/RIF (US$ 13.38) but higher than fluorescence smear microscopy (US$ 0.65). The sensitivity of LAMP was similar to Xpert® MTB/RIF but lower than fluorescence smear microscopy; all three tests had high specificity. These findings support the Malawi policy that recommends a combination of fluorescence smear microscopy and Xpert® MTB/RIF prioritised for people living with HIV, already found to be smear-negative, or being considered for retreatment of tuberculosis.

The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification (LAMP) Assay for Tuberculosis Diagnosis in Adults with Chronic Cough in Malawi

PubMed Central

Nliwasa, Marriott; MacPherson, Peter; Chisala, Palesa; Kamdolozi, Mercy; Khundi, McEwen; Kaswaswa, Kruger; Mwapasa, Mphatso; Msefula, Chisomo; Sohn, Hojoon; Flach, Clare; Corbett, Elizabeth L.

2016-01-01

Background Current tuberculosis diagnostics lack sensitivity, and are expensive. Highly accurate, rapid and cheaper diagnostic tests are required for point of care use in low resource settings with high HIV prevalence. Objective To investigate the sensitivity and specificity, and cost of loop-mediated isothermal amplification (LAMP) assay for tuberculosis diagnosis in adults with chronic cough compared to Xpert® MTB/RIF, fluorescence smear microscopy. Methods Between October 2013 and March 2014, consecutive adults at a primary care clinic were screened for cough, offered HIV testing and assessed for tuberculosis using LAMP, Xpert® MTB/RIF and fluorescence smear microscopy. Sensitivity and specificity (with culture as reference standard), and costs were estimated. Results Of 273 adults recruited, 44.3% (121/273) were HIV-positive and 19.4% (53/273) had bacteriogically confirmed tuberculosis. The sensitivity of LAMP compared to culture was 65.0% (95% CI: 48.3% to 79.4%) with 100% (95% CI: 98.0% to 100%) specificity. The sensitivity of Xpert® MTB/RIF (77.5%, 95% CI: 61.5% to 89.2%) was similar to that of LAMP, p = 0.132. The sensitivity of concentrated fluorescence smear microscopy with routine double reading (87.5%, 95% CI: 73.2% to 95.8%) was higher than that of LAMP, p = 0.020. All three tests had high specificity. The lowest cost per test of LAMP was at batch size of 14 samples (US$ 9.98); this was lower than Xpert® MTB/RIF (US$ 13.38) but higher than fluorescence smear microscopy (US$ 0.65). Conclusion The sensitivity of LAMP was similar to Xpert® MTB/RIF but lower than fluorescence smear microscopy; all three tests had high specificity. These findings support the Malawi policy that recommends a combination of fluorescence smear microscopy and Xpert® MTB/RIF prioritised for people living with HIV, already found to be smear-negative, or being considered for retreatment of tuberculosis. PMID:27171380

A LED-based method for monitoring NAD(P)H and FAD fluorescence in cell cultures and brain slices.

PubMed

Rösner, Jörg; Liotta, Agustin; Schmitz, Dietmar; Heinemann, Uwe; Kovács, Richard

2013-01-30

Nicotinamide- and flavine-adenine-dinucleotides (NAD(P)H and FADH₂) are electron carriers involved in cellular energy metabolism and in a multitude of enzymatic processes. As reduced NAD(P)H and oxidised FAD molecules are fluorescent, changes in tissue auto-fluorescence provide valuable information on the cellular redox state and energy metabolism. Since fluorescence excitation, by mercury arc lamps (HBO) is inherently coupled to photo-bleaching and photo-toxicity, microfluorimetric monitoring of energy metabolism might benefit from the replacement of HBO lamps by light emitting diodes (LEDs). Here we describe a LED-based custom-built setup for monitoring NAD(P)H and FAD fluorescence at the level of single cells (HEK293) and of brain slices. We compared NAD(P)H bleaching characteristics with two light sources (HBO lamp and LED) as well as sensitivity and signal to noise ratio of three different detector types (multi-pixel photon counter (MPPC), photomultiplier tube (PMT) and photodiode). LED excitation resulted in reduced photo-bleaching at the same fluorescence output in comparison to excitation with the HBO lamp. Transiently increasing LED power resulted in reversible bleaching of NAD(P)H fluorescence. Recovery kinetics were dependent on metabolic substrates indicating coupling of NAD(P)H fluorescence to metabolism. Electrical stimulation of brain slices induced biphasic redox changes, as indicated by NAD(P)H/FAD fluorescence transients. Increasing the gain of PMT and decreasing the LED power resulted in similar sensitivity as obtained with the MPPC and the photodiode, without worsening the signal to noise ratio. In conclusion, replacement of HBO lamp with LED might improve conventional PMT based microfluorimetry of tissue auto-fluorescence. Copyright © 2012 Elsevier B.V. All rights reserved.

Magnetic fluorescent lamp

DOEpatents

Berman, S.M.; Richardson R.W.

1983-12-29

The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly is enhanced by providing means for establishing a magnetic field with lines of force along the path of electron flow through the bulb of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

Green-light supplementation for enhanced lettuce growth under red- and blue-light-emitting diodes

NASA Technical Reports Server (NTRS)

Kim, Hyeon-Hye; Goins, Gregory D.; Wheeler, Raymond M.; Sager, John C.

2004-01-01

Plants will be an important component of future long-term space missions. Lighting systems for growing plants will need to be lightweight, reliable, and durable, and light-emitting diodes (LEDs) have these characteristics. Previous studies demonstrated that the combination of red and blue light was an effective light source for several crops. Yet the appearance of plants under red and blue lighting is purplish gray making visual assessment of any problems difficult. The addition of green light would make the plant leave appear green and normal similar to a natural setting under white light and may also offer a psychological benefit to the crew. Green supplemental lighting could also offer benefits, since green light can better penetrate the plant canopy and potentially increase plant growth by increasing photosynthesis from the leaves in the lower canopy. In this study, four light sources were tested: 1) red and blue LEDs (RB), 2) red and blue LEDs with green fluorescent lamps (RGB), 3) green fluorescent lamps (GF), and 4) cool-white fluorescent lamps (CWF), that provided 0%, 24%, 86%, and 51% of the total PPF in the green region of the spectrum, respectively. The addition of 24% green light (500 to 600 nm) to red and blue LEDs (RGB treatment) enhanced plant growth. The RGB treatment plants produced more biomass than the plants grown under the cool-white fluorescent lamps (CWF treatment), a commonly tested light source used as a broad-spectrum control.

Mercury Flow Through the Mercury-Containing Lamp Sector of the Economy of the United States

USGS Publications Warehouse

Goonan, Thomas G.

2006-01-01

Introduction: This Scientific Investigations Report examines the flow of mercury through the mercury-containing lamp sector of the U.S. economy in 2001 from lamp manufacture through disposal or recycling. Mercury-containing lamps illuminate commercial and industrial buildings, outdoor areas, and residences. Mercury is an essential component in fluorescent lamps and high-intensity discharge lamps (high-pressure sodium, mercury-vapor, and metal halide). A typical fluorescent lamp is composed of a phosphor-coated glass tube with electrodes located at either end. Only a very small amount of the mercury is in vapor form. The remainder of the mercury is in the form of either liquid mercury metal or solid mercury oxide (mercury oxidizes over the life of the lamp). When voltage is applied, the electrodes energize the mercury vapor and cause it to emit ultraviolet energy. The phosphor coating absorbs the ultraviolet energy, which causes the phosphor to fluoresce and emit visible light. Mercury-containing lamps provide more lumens per watt than incandescent lamps and, as a result, require from three to four times less energy to operate. Mercury is persistent and toxic within the environment. Mercury-containing lamps are of environmental concern because they are widely distributed throughout the environment and are easily broken in handling. The magnitude of lamp sector mercury emissions, estimated to be 2.9 metric tons per year (t/yr), is small compared with the estimated mercury losses of the U.S. coal-burning and chlor-alkali industries, which are about 70 t/yr and about 90 t/yr, respectively.

Optically trapped atomic resonant devices for narrow linewidth spectral imaging

NASA Astrophysics Data System (ADS)

Qian, Lipeng

This thesis focuses on the development of atomic resonant devices for spectroscopic applications. The primary emphasis is on the imaging properties of optically thick atomic resonant fluorescent filters and their applications. In addition, this thesis presents a new concept for producing very narrow linewidth light as from an atomic vapor lamp pumped by a nanosecond pulse system. This research was motivated by application for missile warning system, and presents an innovative approach to a wide angle, ultra narrow linewidth imaging filter using a potassium vapor cell. The approach is to image onto and collect the fluorescent photons emitted from the surface of an optically thick potassium vapor cell, generating a 2 GHz pass-band imaging filter. This linewidth is narrow enough to fall within a Fraunhefer dark zone in the solar spectrum, thus make the detection solar blind. Experiments are conducted to measure the absorption line shape of the potassium resonant filter, the quantum efficiency of the fluorescent behavior, and the resolution of the fluorescent image. Fluorescent images with different spatial frequency components are analyzed by using a discrete Fourier transform, and the imaging capability of the fluorescent filter is described by its Modulation Transfer Function. For the detection of radiation that is spectrally broader than the linewidth of the potassium imaging filter, the fluorescent image is seen to be blurred by diffuse fluorescence from the slightly off resonant photons. To correct this, an ultra-thin potassium imaging filter is developed and characterized. The imaging property of the ultra-thin potassium imaging cell is tested with a potassium seeded flame, yielding a resolution image of ˜ 20 lines per mm. The physics behind the atomic resonant fluorescent filter is radiation trapping. The diffusion process of the resonant photons trapped in the atomic vapor is theoretically described in this thesis. A Monte Carlo method is used to simulate the absorption and fluorescence. The optimum resolution of the fluorescent image is predicted by simulation. Radiation trapping is also shown to be useful for the generation of ultra-narrow linewidth light from an atomic vapor flash lamp. A 2 nanosecond, high voltage pulse is used to excite low pressure mercury vapor mixed with noble gases, producing high intensity emission at the mercury resonant line at 253.7 nm. With a nanosecond pumping time and high electrical current, the radiation intensity of the mercury discharge is increased significantly compared to a normal glow discharge lamp, while simultaneously suppressing the formation of an arc discharge. By avoiding the arc discharge, discrete spectral lines of mercury were kept at narrow bandwidth. Due to radiation trapping, the emission linewidth from the nanosecond mercury lamp decreases with time and produces ultra-narrow linewidth emission 100 ns after of the excitation, this linewidth is verified by absorption measurements through low pressure mercury absorption filter. The lamp is used along with mercury absorption filters for spectroscopic applications, including Filtered Rayleigh Scattering with different CO2 pressures and Raman scattering from methanol.

Physics of Incandescent Lamp Burnout

ERIC Educational Resources Information Center

Gluck, Paul; King, John

2008-01-01

Incandescent lamps with tungsten filaments have been in use for about a century while being gradually replaced by fluorescent lamps; in another generation both will quite probably be largely replaced by light-emitting diodes. Incandescent lamps (simply called "lamps" in what follows) burn out after a lifetime that depends mostly on the temperature…

Red phosphors for use in high CRI fluorescent lamps

DOEpatents

Srivastava, Alok; Comanzo, Holly; Manivannan, Vankatesan; Setlur, Anant Achyut

2005-11-15

Novel red emitting phosphors for use in fluorescent lamps resulting in superior color rendering index values compared to conventional red phosphors. Also disclosed is a fluorescent lamp including a phosphor layer comprising blends of one or more of a blue phosphor, a blue-green phosphor, a green phosphor and a red a phosphor selected from the group consisting of SrY.sub.2 O.sub.4 :Eu.sup.3+, (Y,Gd)Al.sub.3 B.sub.4 O.sub.12 :Eu.sup.3+, and [(Y.sub.1-x-y-m La.sub.y)Gd.sub.x ]BO.sub.3 :Eu.sub.m wherein y<0.50 and m=0.001-0.3. The phosphor layer can optionally include an additional deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of the disclosed red phosphors in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over the course of the lamp life.

Energy efficient fluorescent ballasts. Phase I, final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stevens Luminoptics Corporation

1978-06-21

The development of a high-frequency electronic (Stevens) ballast for fluorescent lamps is described. It is claimed that use of this ballast could reduce use energy consumption by 1.2 to 2.5 percent. The Stevens ballast has a basic efficiency of 29 percent when used with conventional lamps. With the more efficient lamps, the efficiency increases drastically. The conventional ballast and lamp has an efficiacy of approximately 60 to 63 lumens per watt (LPW). With the Stevens ballast the efficiacy raises to between 75 and 80 lumens per watt. When the Stevens ballast is utilized with the newer high efficiency lamps themore » efficiacy increases to 90 to 95 lumens per watt or a full 51 percent improvement over conventional coil and core ballasts and 25 percent over the best high efficiency premium coil and core ballasts. In addition to its energy savings capabilities, this high frequency fluorescent lamp ballast has the advantages that it is a true retrofit device that is directly interchangeable with the conventional coil core ballast, and it is dimmable over a wide and continuous range. (LCLC)« less

Types of Lamp for Homework and Myopia among Chinese School-Aged Children.

PubMed

Pan, Chen-Wei; Wu, Rong-Kun; Liu, Hu; Li, Jun; Zhong, Hua

2018-06-01

We aim to determine the association of the types of lamp for homework including incandescent lamp, fluorescent lamp, and light-emitting diode (LED) lamp with the prevalence of myopia in Chinese children. 2346 grade 7 students from ten middle schools (93.5% response rate) aged 13 to 14 years in Mojiang, a small county located in Southwestern China, participated in the study. Refractive error was measured with cycloplegia using an autorefractor by optometrists or trained technicians. An IOL Master was used to measure ocular biometric parameters including axial length (AL). Information regarding the types of lamp for homework af``ter schools was collected by questionnaires. Of all the study participants, 693 (29.5%) were affected by myopia, with the prevalence estimates being higher in girls (36.8%; 95% confidence interval [CI]: 34.0, 39.6) than in boys (22.8%; 95% CI: 20.4, 25.1) (P < 0.001). After adjusting for potential confounders such as gender, height, parental history of myopia, time on computer use, time on watching TV, time outdoors, and time on reading and writing, participants using LED lamps for homework had a more myopic refractive error and a longer AL compared with those using incandescent or fluorescent lamps. There were no significant differences in myopia prevalence between children using incandescent and fluorescent lamps for homework. The population attributable risk percentage for myopia associated with using LED lamps for homework after schools was 11.2%. Using LED lamps for homework after schools might contribute to the development of myopia among school-aged children.

Simple luminescence detectors using a light-emitting diode or a Xe lamp, optical fiber and charge-coupled device, or photomultiplier for determining proteins in capillary electrophoresis: a critical comparison.

PubMed

Casado-Terrones, Silvia; Fernández-Sánchez, Jorge F; Segura-Carretero, Antonio; Fernández-Gutiérrez, Alberto

2007-06-01

The performance of two homemade fluorescence-induced capillary electrophoresis detectors, one based on light-emitting diode (LED) as the excitation source and a charge-coupled device (CCD) photodetector and the other based on a commercial luminescence spectrometer (Xe lamp) as the excitation source and a photomultiplier tube as a detector, were compared for the determination of fluorescent proteins R-phycoerythrin and B-phycoerythrin. Both devices use commercially available, reasonably priced optical components that can be used by nonexperts. After fine optimization of several optical and separation parameters in both devices, a zone capillary electrophoresis methodology was achieved with 50mM borate buffer (pH 8.4) and 10mM phytic acid for the determination of two phycobiliproteins. Detection limits of 0.50 and 0.64microg/ml for R-phycoerythrin and B-phycoerythrin, respectively, were achieved by using the LED-induced fluorescence capillary electrophoresis (LED-IF-CE) system, and corresponding detection limits of 2.73 and 2.16microg/ml were achieved by using the Xe lamp-IF-CE system. Analytical performance and other parameters, such as cost and potential to miniaturization, are compared for both devices.

Magnetic fluorescent lamp having reduced ultraviolet self-absorption

DOEpatents

Berman, Samuel M.; Richardson, Robert W.

1985-01-01

The radiant emission of a mercury-argon discharge in a fluorescent lamp assembly (10) is enhanced by providing means (30) for establishing a magnetic field with lines of force along the path of electron flow through the bulb (12) of the lamp assembly, to provide Zeeman splitting of the ultraviolet spectral line. Optimum results are obtained when the magnetic field strength causes a Zeeman splitting of approximately 1.7 times the thermal line width.

Application of a demountable water-cooled hollow-cathode lamp to atomic-fluorescence spectrometry.

PubMed

Rossi, G; Omenetto, N

1969-02-01

A demountable water-cooled hollow-cathode lamp has been investigated as a primary source in atomic fluorescence spectrometry. The discharge current ranged from 300 to 500 mA, and the flowing argon pressure between 0.4 and 4 mbar. Sensitivities ranging from 0.03 to 2 mug ml were obtained for 12 elements. The performances of the hollow-cathode lamp and those of the customary metal vapour discharge lamps for thallium, indium and gallium are compared. The role of the narrowness of the exciting lines in increasing the signal-to-scattering ratios is stressed.

Lighting: The Killer App of Village Power

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1997-12-01

This paper looks at lighting systems as the major market for village level power generation. To the consumer it is something which is needed, could come from a much friendlier source, and the issues of affordability, convenience, and reliability are important. To the supplier lighting has an enormous range of potential customers, it opens the opportunity for other services, and even small demand can give big returns. Because the efficiency of the light source is critical to the number of lights which a fixed power supply can drive, it is important to pick the proper type of bulb to usemore » in this system. The paper discusses test results from an array of fluorescent and incadescent lamps, compared with a kerosene lamp. Low wattage fluorescents seem to perform the best.« less

Determination of mercury distribution inside spent compact fluorescent lamps by atomic absorption spectrometry.

PubMed

Rey-Raap, Natalia; Gallardo, Antonio

2012-05-01

In this study, spent compact fluorescent lamps were characterized to determine the distribution of mercury. The procedure used in this research allowed mercury to be extracted in the vapor phase, from the phosphor powder, and the glass matrix. Mercury concentration in the three phases was determined by the method known as cold vapor atomic absorption spectrometry. Median values obtained in the study showed that a compact fluorescent lamp contained 24.52±0.4ppb of mercury in the vapor phase, 204.16±8.9ppb of mercury in the phosphor powder, and 18.74±0.5ppb of mercury in the glass matrix. There are differences in mercury concentration between the lamps since the year of manufacture or the hours of operation affect both mercury content and its distribution. The 85.76% of the mercury introduced into a compact fluorescent lamp becomes a component of the phosphor powder, while more than 13.66% is diffused through the glass matrix. By washing and eliminating all phosphor powder attached to the glass surface it is possible to classified the glass as a non-hazardous waste. Copyright © 2011 Elsevier Ltd. All rights reserved.

A unique charge-coupled device/xenon arc lamp based imaging system for the accurate detection and quantitation of multicolour fluorescence.

PubMed

Spibey, C A; Jackson, P; Herick, K

2001-03-01

In recent years the use of fluorescent dyes in biological applications has dramatically increased. The continual improvement in the capabilities of these fluorescent dyes demands increasingly sensitive detection systems that provide accurate quantitation over a wide linear dynamic range. In the field of proteomics, the detection, quantitation and identification of very low abundance proteins are of extreme importance in understanding cellular processes. Therefore, the instrumentation used to acquire an image of such samples, for spot picking and identification by mass spectrometry, must be sensitive enough to be able, not only, to maximise the sensitivity and dynamic range of the staining dyes but, as importantly, adapt to the ever changing portfolio of fluorescent dyes as they become available. Just as the available fluorescent probes are improving and evolving so are the users application requirements. Therefore, the instrumentation chosen must be flexible to address and adapt to those changing needs. As a result, a highly competitive market for the supply and production of such dyes and the instrumentation for their detection and quantitation have emerged. The instrumentation currently available is based on either laser/photomultiplier tube (PMT) scanning or lamp/charge-coupled device (CCD) based mechanisms. This review briefly discusses the advantages and disadvantages of both System types for fluorescence imaging, gives a technical overview of CCD technology and describes in detail a unique xenon/are lamp CCD based instrument, from PerkinElmer Life Sciences. The Wallac-1442 ARTHUR is unique in its ability to scan both large areas at high resolution and give accurate selectable excitation over the whole of the UV/visible range. It operates by filtering both the excitation and emission wavelengths, providing optimal and accurate measurement and quantitation of virtually any available dye and allows excellent spectral resolution between different fluorophores. This flexibility and excitation accuracy is key to multicolour applications and future adaptation of the instrument to address the application requirements and newly emerging dyes.

Fluorescent Lamp Replacement Study

DTIC Science & Technology

2017-07-01

friendly products, advances in efficiency, and lower production costs for lamps. The conversion of fluorescent bulbs to LED technology has many benefits ...of 4727 W. An economic analysis was calculated to compare the various lighting technologies that were implemented at ATC and the cost benefits ...the various lighting technologies that were implemented at ATC and the cost benefits of each, a lifecycle comparison was made between the fluorescent

10 CFR Appendix Q to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2014 CFR

2014-01-01

... of Fluorescent Lamp Ballasts Q Appendix Q to Subpart B of Part 430 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY CONSERVATION PROGRAM FOR CONSUMER PRODUCTS Test Procedures Pt. 430, Subpt. B, App. Q Appendix Q to Subpart B of Part 430—Uniform Test Method for Measuring the Energy Consumption of Fluorescent...

10 CFR Appendix Q to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2012 CFR

2012-01-01

... of Fluorescent Lamp Ballasts Q Appendix Q to Subpart B of Part 430 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY CONSERVATION PROGRAM FOR CONSUMER PRODUCTS Test Procedures Pt. 430, Subpt. B, App. Q Appendix Q to Subpart B of Part 430—Uniform Test Method for Measuring the Energy Consumption of Fluorescent...

10 CFR Appendix Q to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2013 CFR

2013-01-01

... of Fluorescent Lamp Ballasts Q Appendix Q to Subpart B of Part 430 Energy DEPARTMENT OF ENERGY ENERGY CONSERVATION ENERGY CONSERVATION PROGRAM FOR CONSUMER PRODUCTS Test Procedures Pt. 430, Subpt. B, App. Q Appendix Q to Subpart B of Part 430—Uniform Test Method for Measuring the Energy Consumption of Fluorescent...

Wood's lamp illumination (image)

MedlinePlus

A Wood's lamp emits ultraviolet light and can be a diagnostic aid in determining if someone has a fungal ... is an infection on the area where the Wood's lamp is illuminating, the area will fluoresce. Normally ...

Comparison of fluorescent and high-pressure sodium lamps on growth of leaf lettuce

NASA Technical Reports Server (NTRS)

Koontz, H. V.; Prince, R. P.; Koontz, R. F.; Knott, W. M. (Principal Investigator)

1987-01-01

Radiation from high-pressure sodium (HPS) lamps provided more than a 50% increased yield (fresh and dry weight of tops) of loose-leaf lettuce cultivars Grand Rapids Forcing and RubyConn, compared to that obtained by radiation from cool-white fluorescent (CWF) lamps at equal photosynthetic photon flux; yet, input wattage was approximately 36% less. It was postulated that the considerable output of 700 to 850 nm radiation from the HPS lamp was a significant factor of the increased yield. Under HPS lamps, the leaves of both cultivars were slightly less green with very little red pigmentation ('RubyConn') and slightly elongated, compared to CWF, but plant productivity per unit electrical energy input was vastly superior with HPS.

10 CFR 430.25 - Laboratory Accreditation Program.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Procedures § 430.25 Laboratory Accreditation Program. The testing for general service fluorescent lamps... Appendix R to this subpart. The testing for medium base compact fluorescent lamps shall be performed in accordance with Appendix W of this subpart. This testing shall be conducted by test laboratories accredited...

10 CFR 430.25 - Laboratory Accreditation Program.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Procedures § 430.25 Laboratory Accreditation Program. The testing for general service fluorescent lamps... Appendix R to this subpart. The testing for medium base compact fluorescent lamps shall be performed in accordance with Appendix W of this subpart. This testing shall be conducted by test laboratories accredited...

Radiation in controlled environments: influence of lamp type and filter material

NASA Technical Reports Server (NTRS)

Bubenheim, D. L.; Bugbee, B.; Salisbury, F. B.

1988-01-01

Radiation in controlled environments was characterized using fluorescent and various high-intensity-discharge (HID) lamps, including metal halide, low-pressure sodium, and high-pressure sodium as the radiation source. The effects of water, glass, or Plexiglas filters on radiation were determined. Photosynthetic photon flux (PPF, 400 to 700 nm), spectra (400 to 1000 nm), shortwave radiation (285-2800 nm), and total radiation (300 to 100,000 nm) were measured, and photosynthetically active radiation (PAR, 400 to 700 nm) and longwave radiation (2800 to 100,000 nm) were calculated. Measurement of PPF alone was not an adequate characterization of the radiation environment. Total radiant flux varied among lamp types at equal PPF. HID lamps provided a lower percentage of longwave radiation than fluorescent lamps, but, when HID lamps provided PPF levels greater than that possible with fluorescent lamps, the amount of longwave radiation was high. Water was the most effective longwave radiation filter. Glass and Plexiglas similarly filtered longwave more than shortwave radiation, but transmission of nonphotosynthetic shortwave radiation was less with Plexiglas than glass. The filter materials tested would not be expected to influence photomorphogenesis because radiation in the action spectrum of phytochrome was not altered, but this may not be the only pigment involved.

Compact Fluorescent Plug-In Ballast-in-a-Socket

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rebecca Voelker

2001-12-21

The primary goal of this program was to develop a ballast system for plug-in CFLs (compact fluorescent lamps) that will directly replace standard metal shell, medium base incandescent lampholders (such as Levition No. 6098) for use with portable lamp fixtures, such as floor, table and desk lamps. A secondary goal was to identify a plug-in CFL that is optimized for use with this ballast. This Plug-in CFL Ballastin-a-Socket system will allow fixture manufacturers to easily manufacture CFL-based high-efficacy portable fixtures that provide residential and commercial consumers with attractive, cost-effective, and energy-efficient fixtures for use wherever portable incandescent fixtures are usedmore » today. The advantages of this proposed system over existing CFL solutions are that the fixtures can only be used with high-efficacy CFLs, and they will be more attractive and will have lower life-cycle costs than screw-in or adapter-based CFL retrofit solutions. These features should greatly increase the penetration of CFL's into the North American market. Our work has shown that using integrated circuits it is quite feasible to produce a lamp-fixture ballast of a size comparable to the current Edison-screw 3-way incandescent fixtures. As for price points for BIAS-based fixtures, end-users polled by the Lighting Research Institute at RPI indicated that they would pay as much as an additional $10 for a lamp containing such a ballast. The ballast has been optimized to run with a 26 W amalgam triple biax lamp in the base-down position, yet can accept non-amalgam versions of the lamp. With a few part alterations, the ballast can be produced to support 32 W lamps as well. The ballast uses GE's existing L-Comp[1] power topology in the circuit so that the integrated circuit design would be a design that could possibly be used by other CFL and EFL products with minor modifications. This gives added value by reducing cost and size of not only the BIAS, but also possibly other integral CFL and future dimmable integral and plug-in versions of the EFL products.« less

Circular, explosion-proof lamp provides uniform illumination

NASA Technical Reports Server (NTRS)

1966-01-01

Circular explosion-proof fluorescent lamp is fitted around a TV camera lens to provide shadowless illumination with a low radiant heat flux. The lamp is mounted in a transparent acrylic housing sealed with clear silicone rubber.

78 FR 7450 - Certain Fluorescent Reflector Lamps and Products and Components Containing Same; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-01

... INTERNATIONAL TRADE COMMISSION [Docket No. 2935] Certain Fluorescent Reflector Lamps and Products... the Public Interest AGENCY: U.S. International Trade Commission. ACTION: Notice. SUMMARY: Notice is hereby given that the U.S. International Trade Commission has received a complaint entitled Certain...

10 CFR 430.25 - Laboratory Accreditation Program.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Procedures § 430.25 Laboratory Accreditation Program. Testing for fluorescent lamp ballasts performed in accordance with appendix Q1 to this subpart shall comply with this § 430.25. The testing for general service... accordance with Appendix R to this subpart. The testing for medium base compact fluorescent lamps shall be...

10 CFR 430.25 - Laboratory Accreditation Program.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Procedures § 430.25 Laboratory Accreditation Program. Testing for fluorescent lamp ballasts performed in accordance with appendix Q1 to this subpart shall comply with this § 430.25. The testing for general service... accordance with Appendix R to this subpart. The testing for medium base compact fluorescent lamps shall be...

16 CFR 305.15 - Labeling for lighting products.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Package labeling. For purposes of labeling under this section, packaging for such fluorescent lamp... individually or in small numbers. The encircled capital letter “E” on packages containing fluorescent lamp... ink, on the surface of the package on which printing or a label normally appears. If the package...

16 CFR 305.15 - Labeling for lighting products.

Code of Federal Regulations, 2013 CFR

2013-01-01

... surface of the fluorescent lamp ballast. (3) Package labeling. For purposes of labeling under this section...” on packages containing fluorescent lamp ballasts or the luminaires into which they are incorporated must appear conspicuously, in color-contrasting ink, on the surface of the package on which printing or...

16 CFR 305.15 - Labeling for lighting products.

Code of Federal Regulations, 2010 CFR

2010-01-01

... surface of the fluorescent lamp ballast. (3) Package labeling. For purposes of labeling under this section...” on packages containing fluorescent lamp ballasts or the luminaires into which they are incorporated must appear conspicuously, in color-contrasting ink, on the surface of the package on which printing or...

16 CFR 305.15 - Labeling for lighting products.

Code of Federal Regulations, 2014 CFR

2014-01-01

... surface of the fluorescent lamp ballast. (3) Package labeling. For purposes of labeling under this section...” on packages containing fluorescent lamp ballasts or the luminaires into which they are incorporated must appear conspicuously, in color-contrasting ink, on the surface of the package on which printing or...

16 CFR 305.15 - Labeling for lighting products.

Code of Federal Regulations, 2012 CFR

2012-01-01

... surface of the fluorescent lamp ballast. (3) Package labeling. For purposes of labeling under this section...” on packages containing fluorescent lamp ballasts or the luminaires into which they are incorporated must appear conspicuously, in color-contrasting ink, on the surface of the package on which printing or...

Blue-green phosphor for fluorescent lighting applications

DOEpatents

Srivastava, Alok; Comanzo, Holly; Manivannan, Venkatesan; Setlur, Anant Achyut

2005-03-15

A fluorescent lamp including a phosphor layer including Sr.sub.4 Al.sub.14 O.sub.25 :Eu.sup.2+ (SAE) and at least one of each of a red, green and blue emitting phosphor. The phosphor layer can optionally include an additional, deep red phosphor and a yellow emitting phosphor. The resulting lamp will exhibit a white light having a color rendering index of 90 or higher with a correlated color temperature of from 2500 to 10000 Kelvin. The use of SAE in phosphor blends of lamps results in high CRI light sources with increased stability and acceptable lumen maintenance over, the course of the lamp life.

Management of fluorescent lamps in controlled environment chambers

NASA Technical Reports Server (NTRS)

Romer, Mark

1994-01-01

Management of fluorescent lights is recommended to (1) maintain uniformity of light intensity over time and (2) permit reproducibility of lighting conditions during experimental replications. At the McGill Phytotron, the lighting intensity can be controlled to desired level because any individual pair of the 40 lamps in each chamber can be set to be 'on' at any particular time. A lamp canopy service history is maintained for each experiment permitting accurate replication of lighting conditions for subsequent replicate trials.

Cold starting of fluorescent lamps - part I: a description of the transient regime

NASA Astrophysics Data System (ADS)

Langer, Reinhard; Garner, Richard; Paul, Irina; Horn, Siegfried; Tidecks, Reinhard

2016-10-01

In this paper we give a proposal for the transient behaviour of a cold-started fluorescent lamp, from the generation of the first conductive channel over the normal and abnormal glow discharge and the glow-to-arc (GTA) transition to the arc discharge in the steady state. Starting from the equilibrium voltage-current characteristics of the lamp and considering recent experimental results a qualitative description of the transient regime is developed, which was so far not available in the literature.

A mercury arc lamp-based multi-color confocal real time imaging system for cellular structure and function.

PubMed

Saito, Kenta; Kobayashi, Kentaro; Tani, Tomomi; Nagai, Takeharu

2008-01-01

Multi-point scanning confocal microscopy using a Nipkow disk enables the acquisition of fluorescent images with high spatial and temporal resolutions. Like other single-point scanning confocal systems that use Galvano meter mirrors, a commercially available Nipkow spinning disk confocal unit, Yokogawa CSU10, requires lasers as the excitation light source. The choice of fluorescent dyes is strongly restricted, however, because only a limited number of laser lines can be introduced into a single confocal system. To overcome this problem, we developed an illumination system in which light from a mercury arc lamp is scrambled to make homogeneous light by passing it through a multi-mode optical fiber. This illumination system provides incoherent light with continuous wavelengths, enabling the observation of a wide range of fluorophores. Using this optical system, we demonstrate both the high-speed imaging (up to 100 Hz) of intracellular Ca(2+) propagation, and the multi-color imaging of Ca(2+) and PKC-gamma dynamics in living cells.

Serious complications in experiments in which UV doses are effected by using different lamp heights.

PubMed

Flint, Stephan D; Ryel, Ronald J; Hudelson, Timothy J; Caldwell, Martyn M

2009-10-06

Many experiments examining plant responses to enhanced ultraviolet-B radiation (280-315nm) simply compare an enhanced UV-B treatment with ambient UV-B (or no UV-B radiation in most greenhouse and controlled-environment studies). Some more detailed experiments utilize multiple levels of UV-B radiation. A number of different techniques have been used to adjust the UV dose. One common technique is to place racks of fluorescent UV-emitting lamps at different heights above the plant canopy. However, the lamps and associated support structure cast shadows on the plant bed below. We calculated one example of the sequence of shade intervals for two common heights of lamp racks and show the patterns and duration of shade which the plants receive is distributed differently over the course of the day for different heights of the lamp racks. We also conducted a greenhouse experiment with plants (canola, sunflower and maize) grown under unenergized lamp racks suspended at the same two heights above the canopy. Growth characteristics differed in unpredictable ways between plants grown under the two heights of lamp racks. These differences could enhance or obscure potential UV-B effects. Also, differences in leaf mass per unit foliage area, which were observed in this experiment, could contribute to differences in plant UV-B sensitivity. We recommend the use of other techniques for achieving multiple doses of UV-B radiation. These range from simple and inexpensive approaches (e.g., wrapping individual fluorescent tubes in layers of a neutral-density filter such as cheese cloth) to more technical and expensive alternatives (e.g., electronically modulated lamp control systems). These choices should be determined according to the goals of the particular experiment.

Optical radiation emissions from compact fluorescent lamps.

PubMed

Khazova, M; O'Hagan, J B

2008-01-01

There is a drive to energy efficiency to mitigate climate change. To meet this challenge, the UK Government has proposed phasing out incandescent lamps by the end of 2011 and replacing them with energy efficient fluorescent lighting, including compact fluorescent lamps (CFLs) with integrated ballasts. This paper presents a summary of an assessment conducted by the Health Protection Agency in March 2008 to evaluate the optical radiation emissions of CFLs currently available in the UK consumer market. The study concluded that the UV emissions from a significant percentage of the tested CFLs with single envelopes may result in foreseeable overexposure of the skin when these lamps are used in desk or task lighting applications. The optical output of all tested CFLs, in addition to high-frequency modulation, had a 100-Hz envelope with modulation in excess of 15%. This degree of modulation may be linked to a number of adverse effects.

Fluorescent and high intensity discharge lamp use in chambers and greenhouses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Langhans, R.W.

1994-12-31

Fluorescent and High Intensity Discharge lamps have opened up great opportunities for researchers to study plant growth under controlled environment conditions and for commercial growers to increase plant production during low/light periods. This report describes the advantages and disadvantages of using each lamp in growth chambers, growth rooms and greenhouses. Growth Chambers are small (3m x 4/m and smaller) walk-in or reach-in enclosures with programmable, accurate temperature, relative humidity (RH) and irradiance control over wide ranges. The intent of growth chambers was to replicate sunlight conditions and transfer research results directly to the greenhouse or outside. It was realized thatmore » sunlight and outside conditions could not be mimicked. Growth chambers are also used to study irradiance and spectral fluxes. Growth Rooms are usually large rooms (larger than 3m x 4m) with only lamp irradiance, but providing relatively limited ranges of environmental control (i.e., 10 to 30 C temperature, 50 to 90% RH and ambient to 1000 ppm CO{sub 2}), and commonly independent of outside conditions. Irradiance requirements for growth rooms are similar to those of growth chambers. Growth rooms are also used for growing a large number of plants in a uniform standard environment condition and in commercial horticulture for tissue culture, seed germination (plugs) and seedling growth. Greenhouses are designed to allow maximum sunlight penetration through the structure. Initially greenhouses were used to extend the growing season. Then as heating systems, and cooling systems improved, they were used year round. Low light during the winter months reduced plant growth, but with the advent of efficient lamps (HID and fluorescent) it became possible to increase growth to rates close to that in summer months. Supplementary lighting is used during low light periods of the year and anytime to ensure consistent total daily irradiance for research plants.« less

High intensity portable fluorescent light

NASA Technical Reports Server (NTRS)

Kendall, F. B.

1972-01-01

Eight high intensity portable fluorescent lights were produced. Three prototype lights were also produced, two of which were subsequently updated to the physical and operational configuration of the qualification and flight units. Positioning of lamp apertures and reflectors in these lights is such that the light is concentrated and intensified in a specific pattern rather than widely diffused. Indium amalgam control of mercury vapor pressure in the lamp gives high output at lamp ambient temperatures up to 105 C. A small amount of amalgam applied to each electrode stem helps to obtain fast warm-up. Shrinking a Teflon sleeve on the tube and potting metal caps on each end of the lamp minimizes dispersion of mercury vapor and glass particles in the event of accidental lamp breakage. Operation at 20 kHz allows the lamps to consume more power than at low frequency, thus increasing their light output and raising their efficiency. When used to expose color photographic film, light from the lamps produces results approximately equal to sunlight.

Frequent Questions About EPA's Mercury Lamp Drum-Top Crusher Study

EPA Pesticide Factsheets

Frequent Questions such as Why did we do the Mercury Lamp Drum-Top Crusher Study?, Should drum-top crushers (DTCs) be used in the management of fluorescent lamps?, Are drum-top crushers (DTCs) safe to use?

The Photoluminescence of a Fluorescent Lamp: Didactic Experiments on the Exponential Decay

ERIC Educational Resources Information Center

Onorato, Pasquale; Gratton, Luigi; Malgieri, Massimiliano; Oss, Stefano

2017-01-01

The lifetimes of the photoluminescent compounds contained in the coating of fluorescent compact lamps are usually measured using specialised instruments, including pulsed lasers and/or spectrofluorometers. Here we discuss how some low cost apparatuses, based on the use of either sensors for the educational lab or commercial digital photo cameras,…

The Effect of Lighting on the Behavior of Children Who Are Developmentally Disabled.

ERIC Educational Resources Information Center

Shapiro, Michele; Roth, Dana; Marcus, Angela

2001-01-01

A study examined the behavior of 8 Israeli children (ages 2-4) with developmental disabilities and maladaptive behavior who were exposed to direct lighting with unshielded standard fluorescent lamps vs. indirect diffuse full spectrum fluorescent lamps. The duration of adaptive behaviors was significantly higher under the indirect lighting.…

HIGHLY BRIGHT, HEAVY METAL-FREE AND STABLE DOPED SEMICONDUCTOR NANO-PHOSPHORS FOR ECONOMICAL SOLID STATE LIGHTING ALTERNATIVES - PHASE II

EPA Science Inventory

There is growing concern about how to limit the release of mercury into the environment. One significant source of mercury is found in fluorescent lamps. Recently, however, compact fluorescent lamps have been heavily promoted in order to conserve electrical energy. While it...

Systems of artificial lighting at the Phytotron of Plant Breeding and Genetic Institute (Odessa)

NASA Technical Reports Server (NTRS)

Chernozubov, Adolf

1994-01-01

At the Odessa Phytotron we have installed over 50 climatic chambers and cabinets made by various companies of the United States, Canada, Germany and U.S.S.R. They employ different light sources including Sylvania fluorescent lamps of various types, fluorescent lamps produced in the former Soviet Union with a special luminophore, ordinary tungsten lamps, xenon, mercury, mercury-iodide, sodium, etc. Our objective in lighting is that the intensity distribution over the wave lengths should be maximal in the photosynthetically active part of the spectrum and minimal in the IR part to avoid plant sterilization. Phytotrons are extremely energy consuming entities, and the large part of their energy consumption falls into the lighting category in our electric bills. Therefore, we are in a constant search of the processes to reduce energy. However, the main way to increase effectiveness would be the development of new types of light sources, which would come close to the threshold of 150 to 200 lumens per watt.

Dimmable electronic ballasts by variable power density modulation technique

NASA Astrophysics Data System (ADS)

Borekci, Selim; Kesler, Selami

2014-11-01

Dimming can be accomplished commonly by switching frequency and pulse density modulation techniques and a variable inductor. In this study, a variable power density modulation (VPDM) control technique is proposed for dimming applications. A fluorescent lamp is operated in several states to meet the desired lamp power in a modulation period. The proposed technique has the same advantages of magnetic dimming topologies have. In addition, a unique and flexible control technique can be achieved. A prototype dimmable electronic ballast is built and experiments related to it have been conducted. As a result, a 36WT8 fluorescent lamp can be driven for a desired lamp power from several alternatives without modulating the switching frequency.

A flash-lamp based device for fluorescence detection and identification of individual pollen grains.

PubMed

Kiselev, Denis; Bonacina, Luigi; Wolf, Jean-Pierre

2013-03-01

We present a novel optical aerosol particle detector based on Xe flash lamp excitation and spectrally resolved fluorescence acquisition. We demonstrate its performances on three natural pollens acquiring in real-time scattering intensity at two wavelengths, sub-microsecond time-resolved scattering traces of the particles' passage in the focus, and UV-excited fluorescence spectra. We show that the device gives access to a rather specific detection of the bioaerosol particles.

Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes.

PubMed

Gadkar, Vijay J; Goldfarb, David M; Gantt, Soren; Tilley, Peter A G

2018-04-03

Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification (iNAAT) technique known for its simplicity, sensitivity and speed. Its low-cost feature has resulted in its wide scale application, especially in low resource settings. The major disadvantage of LAMP is its heavy reliance on indirect detection methods like turbidity and non-specific dyes, which often leads to the detection of false positive results. In the present work, we have developed a direct detection approach, whereby a labelled loop probe quenched in its unbound state, fluoresces only when bound to its target (amplicon). Henceforth, referred to as Fluorescence of Loop Primer Upon Self Dequenching-LAMP (FLOS-LAMP), it allows for the sequence-specific detection of LAMP amplicons. The FLOS-LAMP concept was validated for rapid detection of the human pathogen, Varicella-zoster virus, from clinical samples. The FLOS-LAMP had a limit of detection of 500 copies of the target with a clinical sensitivity and specificity of 96.8% and 100%, respectively. The high level of specificity is a major advance and solves one of the main shortcomings of the LAMP technology, i.e. false positives. Self-quenching/de-quenching probes were further used with other LAMP primer sets and different fluorophores, thereby demonstrating its versatility and adaptability.

Most Probable Number - Loop Mediated Isothermal Amplification (MPN-LAMP) for Quantifying Waterborne Pathogens in Less Than 25 Minutes

PubMed Central

Ahmad, Farhan; Stedtfeld, Robert D.; Waseem, Hassan; Williams, Maggie R.; Cupples, Alison M.; Tiedje, James M.; Hashsham, Syed A.

2016-01-01

We are reporting a most probable number approach integrated to loop mediated isothermal technique (MPN-LAMP) focusing on Gram-negative Escherichia coli and Gram-positive Enterococcus faecalis bacterial cells without nucleic acids extraction. LAMP assays for uidA from E. coli and gelE from E. faecalis were successfully performed directly on cells up to single digit concentration using a commercial real time PCR instrument. Threshold time values of LAMP assays of bacterial cells, heat treated bacterial cells (95 °C for 5 min), and their purified genomic DNA templates were similar, implying that amplification could be achieved directly from bacterial cells at 63 °C. Viability of bacterial cells was confirmed by using propidium monoazide in a LAMP assay with E. faecalis. To check its functionality on a microfluidic platform, MPN-LAMP assays targeting < 10 CFU of bacteria were also translated onto polymeric microchips and monitored by a low-cost fluorescence imaging system. The overall system provided signal-to-noise (SNR) ratios up to 800, analytical sensitivity of < 10 CFU, and time to positivity of about 20 min. MPN-LAMP assays were performed for cell concentrations in the range of 105 CFU to < 10 CFU. MPN values from LAMP assays confirmed that the amplifications were from < 10 CFU. The method described here, applicable directly on cells at 63 °C, eliminates the requirement of complex nucleic acids extraction steps, facilitating the development of sensitive, rapid, low-cost, and field-deployable systems. This rapid MPN-LAMP approach has the potential to replace conventional MPN method for waterborne pathogens. PMID:27856278

Integrated starting and running amalgam assembly for an electrodeless fluorescent lamp

DOEpatents

Borowiec, Joseph Christopher; Cocoma, John Paul; Roberts, Victor David

1998-01-01

An integrated starting and running amalgam assembly for an electrodeless SEF fluorescent lamp includes a wire mesh amalgam support constructed to jointly optimize positions of a starting amalgam and a running amalgam in the lamp, thereby optimizing mercury vapor pressure in the lamp during both starting and steady-state operation in order to rapidly achieve and maintain high light output. The wire mesh amalgam support is constructed to support the starting amalgam toward one end thereof and the running amalgam toward the other end thereof, and the wire mesh is rolled for friction-fitting within the exhaust tube of the lamp. The positions of the starting and running amalgams on the wire mesh are jointly optimized such that high light output is achieved quickly and maintained, while avoiding any significant reduction in light output between starting and running operation.

The Radiative Decay of Green and Red Photoluminescent Phosphors: An Undergraduate Kinetics Experiment for Materials Chemistry

ERIC Educational Resources Information Center

Esposti, C. Degli; Bizzocchi, L.

2008-01-01

This article describes a laboratory experiment that allows the students to investigate the radiative properties of the green and red emitting phosphors that are employed in commercial fluorescent lamps. Making use of a spectrofluorometer, students first record the emission spectrum of a fluorescent lamp under normal operating conditions, and then…

Lamp pumped Nd:YAG laser. Space-qualifiable Nd:YAG laser for optical communications

NASA Technical Reports Server (NTRS)

Ward, K. B.

1973-01-01

Results are given of a program concerned with the design, fabrication, and evaluation of alkali pump lamps for eventual use in a space qualified Nd:YAG laser system. The study included evaluation of 2mm through 6mm bore devices. Primary emphasis was placed upon the optimization of the 4mm bore lamp and later on the 6mm bore lamp. As part of this effort, reference was made to the Sylvania work concerned with the theoretical modeling of the Nd:YAG laser. With the knowledge gained, a projection of laser performance was made based upon realistic lamp parameters which should easily be achieved during following developmental efforts. Measurements were made on the lamp performance both in and out of the cavity configuration. One significant observation was that for a constant vapor pressure device, the spectral and fluorescent output did not vary for vacuum or argon environment. Therefore, the laser can be operated in an inert environment (eg. argon) with no degradation in output. Laser output of 3.26 watts at 430 watts input was obtained for an optimized 4mm bore lamp.

Monomeric fluorescent timers that change color from blue to red report on cellular trafficking.

PubMed

Subach, Fedor V; Subach, Oksana M; Gundorov, Illia S; Morozova, Kateryna S; Piatkevich, Kiryl D; Cuervo, Ana Maria; Verkhusha, Vladislav V

2009-02-01

Based on the mechanism for chromophore formation in red fluorescent proteins, we developed three mCherry-derived monomeric variants, called fluorescent timers (FTs), that change their fluorescence from the blue to red over time. These variants exhibit distinctive fast, medium and slow blue-to-red chromophore maturation rates that depend on the temperature. At 37 degrees C, the maxima of the blue fluorescence are observed at 0.25, 1.2 and 9.8 h for the purified fast-FT, medium-FT and slow-FT, respectively. The half-maxima of the red fluorescence are reached at 7.1, 3.9 and 28 h, respectively. The FTs show similar timing behavior in bacteria, insect and mammalian cells. Medium-FT allowed for tracking of the intracellular dynamics of the lysosome-associated membrane protein type 2A (LAMP-2A) and determination of its age in the targeted compartments. The results indicate that LAMP-2A transport through the plasma membrane and early or recycling endosomes to lysosomes is a major pathway for LAMP-2A trafficking.

Rapid and reliable diagnostic method to detect Zika virus by real-time fluorescence reverse transcription loop-mediated isothermal amplification.

PubMed

Guo, Xu-Guang; Zhou, Yong-Zhuo; Li, Qin; Wang, Wei; Wen, Jin-Zhou; Zheng, Lei; Wang, Qian

2018-04-18

To detect Zika virus more rapidly and accurately, we developed a novel method that utilized a real-time fluorescence reverse transcription loop-mediated isothermal amplification (LAMP) technique. The NS5 gene was amplified by a set of six specific primers that recognized six distinct sequences. The amplification process, including 60 min of thermostatic reaction with Bst DNA polymerase following real-time fluorescence reverse transcriptase using genomic Zika virus standard strain (MR766), was conducted through fluorescent signaling. Among the six pairs of primers that we designate here, NS5 was the most efficient with a high sensitivity of up to 3.3 ng/μl and reproducible specificity on eight pathogen samples that were used as negative controls. The real-time fluorescence reverse transcription LAMP detection process can be completed within 35 min. Our study demonstrated that real-time fluorescence reverse transcription LAMP could be highly beneficial and convenient clinical application to detect Zika virus due to its high specificity and stability.

Light transmission and ultraviolet protection of contact lenses under artificial illumination.

PubMed

Artigas, José M; Navea, Amparo; García-Domene, M Carmen; Gené, Andrés; Artigas, Cristina

2016-04-01

To determine the spectral transmission of contact lenses (CLs), with and without an ultraviolet (UV) filter to evaluate their capacity for protection under UV radiation from artificial illumination (incandescent, fluorescent, xenon (Xe) lamps, or white LEDs (light-emitting diode)). The transmission curves of nine soft CLs were obtained by using a PerkinElmer Lambda 35 UV-vis spectrophotometer. A CIE standard was used for the emission spectra of incandescent and fluorescent lamps, and Xe lamps and white LEDs were measured by using an International Light Technologies ILT-950 spectroradiometer. Five of the nine soft CLs analysed state that they incorporate UV filters, but the other four do not specify anything in this regard. The spectral transmission of all the CLs studied is excellent in the visible region. The CLs with UV filters filter out this radiation more or less effectively. Xe lamps emit a part in the UV region. Incandescent, fluorescent and white LEDs do not emit at all in the UV. Incorporating UV filters is important when the illumination is from a Xe lamp since this light source emits in the UV region. This, however, does not occur with incandescent and fluorescent lamps or white LEDs. The CLs that do incorporate UV filters meet all the standard requirements that the U.S. FDA (Food and Drug Administration) has for UV-blocking CLs Class II (OcularScience, CooperVision and Neolens), and AcuvueMoist and HydronActifresh400 even comply with the stricter Class I. The CLs without UV filters let UVA, UVB and even some UVC through. Copyright © 2015. Published by Elsevier Ltd.

Heat-Transfer in Reflector-type Self-Ballasted Compact Fluorescent Lamps

NASA Astrophysics Data System (ADS)

Yasuda, Takeo; Toda, Masahiro; Matsumoto, Shinichiro; Takahara, Yuichiro

Self-ballasted compact fluorescent lamps (SBCFL) are widely used to replace incandescent lamps (IL) to save energy. We studied the heat-transfer phenomena of SBCFLs with outer envelopes by measuring the temperatures of the lamp parts, the power consumption, and the luminous output, and by calculating the energy balance. The methods applied were heat-transfer network analysis and computational fluid dynamics (CFD) using FLUENT® software. The heat loss increased in reflector-type SBCFLs as compared to SBCFLs with non-reflective outer envelopes, and was estimated at about 3 W when the total lamp power was 22 W. This results in a temperature rise of 20 K in the plastic holder, and a maximum rise of 10 K at the electronic components on the circuit board. Accordingly, we have developed a 12 W reflector-type SBCFL, which replaces a 60 W incandescent, not a 22 W SBCFL replacing a 100 W incandescent R-lamp, due to the importance of thermal reliability.

Thermal element for maintaining minimum lamp wall temperature in fluorescent fixtures

DOEpatents

Siminovitch, Michael J.

1992-01-01

In a lighting fixture including a lamp and a housing, an improvement is disclosed for maintaining a lamp envelope area at a cooler, reduced temperature relative to the enclosed housing ambient. The improvement comprises a thermal element in thermal communication with the housing extending to and springably urging thermal communication with a predetermined area of the lamp envelope surface.

Deep ultraviolet semiconductor light sources for sensing and security

NASA Astrophysics Data System (ADS)

Shatalov, Max; Bilenko, Yuri; Yang, Jinwei; Gaska, Remis

2009-09-01

III-Nitride based deep ultraviolet (DUV) light emitting diodes (LEDs) rapidly penetrate into sensing market owing to several advantages over traditional UV sources (i.e. mercury, xenon and deuterium lamps). Small size, a wide choice of peak emission wavelengths, lower power consumption and reduced cost offer flexibility to system integrators. Short emission wavelength offer advantages for gas detection and optical sensing systems based on UV induced fluorescence. Large modulation bandwidth for these devices makes them attractive for frequency-domain spectroscopy. We will review present status of DUV LED technology and discuss recent advances in short wavelength emitters and high power LED lamps.

Detoxification of mercury pollutant leached from spent fluorescent lamps using bacterial strains.

PubMed

Al-Ghouti, Mohammad A; Abuqaoud, Reem H; Abu-Dieyeh, Mohammed H

2016-03-01

The spent fluorescent lamps (SFLs) are being classified as a hazardous waste due to having mercury as one of its main components. Mercury is considered the second most toxic heavy metal (arsenic is the first) with harmful effects on animal nervous system as it causes different neurological disorders. In this research, the mercury from phosphor powder was leached, then bioremediated using bacterial strains isolated from Qatari environment. Leaching of mercury was carried out with nitric and hydrochloric acid solutions using two approaches: leaching at ambient conditions and microwave-assisted leaching. The results obtained from this research showed that microwave-assisted leaching method was significantly better in leaching mercury than the acid leaching where the mercury leaching efficiency reached 76.4%. For mercury bio-uptake, twenty bacterial strains (previously isolated and purified from petroleum oil contaminated soils) were sub-cultured on Luria Bertani (LB) plates with mercury chloride to check the bacterial tolerance to mercury. Seven of these twenty strains showed a degree of tolerance to mercury. The bio-uptake capacities of the promising strains were investigated using the mercury leached from the fluorescent lamps. Three of the strains (Enterobacter helveticus, Citrobacter amalonaticus, and Cronobacter muytjensii) showed bio-uptake efficiency ranged from 28.8% to 63.6%. Copyright © 2015 Elsevier Ltd. All rights reserved.

Absolute Doppler shift calibration of laser induced fluorescence signals using optogalvanic measurements in a hollow cathode lamp

NASA Technical Reports Server (NTRS)

Ruyten, Wilhelmus M.; Keefer, Dennis

1992-01-01

The paper investigates the use of optogalvanic (OG) measurements on the neutral 3P1 and 3P2 levels of argon in a hollow cathode lamp for the purpose of calibrating Doppler shifts of laser-induced fluorescence signals from an arcjet plume. It is shown that, even with non-Doppler-free OG detection, accuracy to better than 10 MHz is possible but that, depending on the experiment geometry, corrections of 10-35 MHz may be necessary to offset small axial drift velocities of neutral atoms in the hollow cathode lamp.

Monitoring of drugs and metabolites in body fluids by capillary electrophoresis with XeHg lamp-based and laser-induced fluorescence detection.

PubMed

Caslavska, Jitka; Thormann, Wolfgang

2004-06-01

Commercial capillary electrophoresis instrumentation with XeHg lamp-based and laser induced fluorescence (LIF) detection is employed for analysis of urinary 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and its major metabolites, urinary metabolites of acetylsalicylic acid, urinary benzoylecgonine in an immunoassay format, and albendazole sulfoxide and albendazole sulfone in plasma. For the examples studied, the data suggest that the lamp-based detector can be employed for the monitoring of pharmacological and toxicological relevant solute concentrations, and thus represents an attractive alternative to LIF detection.

Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min.

PubMed

Ahmad, Farhan; Stedtfeld, Robert D; Waseem, Hassan; Williams, Maggie R; Cupples, Alison M; Tiedje, James M; Hashsham, Syed A

2017-01-01

We are reporting a most probable number approach integrated to loop mediated isothermal technique (MPN-LAMP) focusing on Gram-negative Escherichia coli and Gram-positive Enterococcus faecalis bacterial cells without nucleic acids extraction. LAMP assays for uidA from E. coli and gelE from E. faecalis were successfully performed directly on cells up to single digit concentration using a commercial real time PCR instrument. Threshold time values of LAMP assays of bacterial cells, heat treated bacterial cells (95°C for 5min), and their purified genomic DNA templates were similar, implying that amplification could be achieved directly from bacterial cells at 63°C. Viability of bacterial cells was confirmed by using propidium monoazide in a LAMP assay with E. faecalis. To check its functionality on a microfluidic platform, MPN-LAMP assays targeting <10CFU of bacteria were also translated onto polymeric microchips and monitored by a low-cost fluorescence imaging system. The overall system provided signal-to-noise (SNR) ratios up to 800, analytical sensitivity of <10CFU, and time to positivity of about 20min. MPN-LAMP assays were performed for cell concentrations in the range of 10 5 CFU to <10CFU. MPN values from LAMP assays confirmed that the amplifications were from <10CFU. The method described here, applicable directly on cells at 63°C, eliminates the requirement of complex nucleic acids extraction steps, facilitating the development of sensitive, rapid, low-cost, and field-deployable systems. This rapid MPN-LAMP approach has the potential to replace conventional MPN method for waterborne pathogens. Copyright © 2016 Elsevier B.V. All rights reserved.

Thermal element for maintaining minimum lamp wall temperature in fluorescent fixtures

DOEpatents

Siminovitch, M.J.

1992-11-10

In a lighting fixture including a lamp and a housing, an improvement is disclosed for maintaining a lamp envelope area at a cooler, reduced temperature relative to the enclosed housing ambient. The improvement comprises a thermal element in thermal communication with the housing extending to and springably urging thermal communication with a predetermined area of the lamp envelope surface. 12 figs.

10 CFR Appendix Q to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2010 CFR

2010-01-01

... fluorescent lamp is designed to operate. 1.14PLC control signal means a power line carrier (PLC) signal that....3Power Line Carrier (PLC) Control Signal. Measure the PLC control signal power (watts), using a wattmeter... have a frequency response that is at least 10 times higher than the PLC being measured in order to...

10 CFR Appendix Q to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Fluorescent Lamp Ballasts

Code of Federal Regulations, 2011 CFR

2011-01-01

... fluorescent lamp is designed to operate. 1.14PLC control signal means a power line carrier (PLC) signal that....3Power Line Carrier (PLC) Control Signal. Measure the PLC control signal power (watts), using a wattmeter... have a frequency response that is at least 10 times higher than the PLC being measured in order to...

Early Development of Cloned Bovine Embryos Produced from Oocytes Enucleated by Fluorescence Metaphase II Imaging Using a Conventional Halogen-Lamp Microscope

PubMed Central

Iwamoto, Daisaku; Yamagata, Kazuo; Kishi, Masao; Hayashi-Takanaka, Yoko; Kimura, Hiroshi; Wakayama, Teruhiko

2015-01-01

Abstract Enucleation of a recipient oocyte is one of the key processes in the procedure of somatic cell nuclear transfer (SCNT). However, especially in bovine species, lipid droplets spreading in the ooplasm hamper identification and enucleation of metaphase II (MII) chromosomes, and thereby the success rate of the cloning remains low. In this study we used a new experimental system that enables fluorescent observation of chromosomes in living oocytes without any damage. We succeeded in visualizing and removing the MII chromosome in matured bovine oocytes. This experimental system consists of injecting fluorescence-labeled antibody conjugates that bind to chromosomes and fluorescent observation using a conventional halogen-lamp microscope. The cleavage rates and blastocyst rates of bovine embryos following in vitro fertilization (IVF) decreased as the concentration of the antibody increased (p<0.05). The enucleation rate of the conventional method (blind enucleation) was 86%, whereas all oocytes injected with the antibody conjugates were enucleated successfully. Fusion rates and developmental rates of SCNT embryos produced with the enucleated oocytes were the same as those of the blind enucleation group (p>0.05). For the production of SCNT embryos, the new system can be used as a reliable predictor of the location of metaphase plates in opaque oocytes, such as those in ruminant animals. PMID:25826723

Color Degradation of Textiles with Natural Dyes and of Blue Scale Standards Exposed to White LED Lamps:Evaluation of White LED Lamps for Effectiveness as Museum Lighting

NASA Astrophysics Data System (ADS)

Ishii, Mie; Moriyama, Takayoshi; Toda, Masahiro; Kohmoto, Kohtaro; Saito, Masako

White light-emitting diodes (LED) are well suited for museum lighting because they emit neither UV nor IR radiation, which damage artifacts. The color degradation of natural dyes and blue scale standards (JIS L 0841) by white LED lamps are examined, and the performance of white LED lamps for museum lighting is evaluated. Blue scale standard grades 1-6 and silk fabrics dyed with 22 types of natural dyes classified as mid to highly responsive in a CIE technical report (CIE157:2004) were exposed to five types of white LED lamps using different luminescence methods and color temperatures. Color changes were measured at each 15000 lx·hr (500 lx at fabric surface × 300 hr) interval ten times. The accumulated exposure totaled 150000 lx·hr. The data on conventional white LED lamps and previously reported white fluorescent (W) and museum fluorescent (NU) lamps was evaluated. All the white LED lamps showed lower fading rates compared with a W lamp on a blue scale grade 1. The fading rate of natural dyes in total was the same between an NU lamp (3000 K) and a white LED lamp (2869 K). However, yellow natural dyes showed higher fading rates with the white LED lamp. This tendency is due to the high power characteristic of the LED lamp around 400-500 nm, which possibly contributes to the photo-fading action on the dyes. The most faded yellow dyes were Ukon (Curcuma longa L.) and Kihada (Phellodendron amurense Rupr.), and these are frequently used in historic artifacts such as kimono, wood-block prints, and scrolls. From a conservation point of view, we need to continue research on white LED lamps for use in museum lighting.

A new phenomenon?

NASA Astrophysics Data System (ADS)

Ganci, S.

2017-03-01

This letter describes an interesting optical phenomenon engaging the reflected images of a new tube LED lamp (an array of high luminosity LEDs substituting the old fluorescent lamps) on any convex glossy black surface.

Effects of artificial lighting on the detection of plant stress with spectral reflectance remote sensing in bioregenerative life support systems

NASA Astrophysics Data System (ADS)

Schuerger, Andrew C.; Richards, Jeffrey T.

2006-09-01

Plant-based life support systems that utilize bioregenerative technologies have been proposed for long-term human missions to both the Moon and Mars. Bioregenerative life support systems will utilize higher plants to regenerate oxygen, water, and edible biomass for crews, and are likely to significantly lower the ‘equivalent system mass’ of crewed vehicles. As part of an ongoing effort to begin the development of an automatic remote sensing system to monitor plant health in bioregenerative life support modules, we tested the efficacy of seven artificial illumination sources on the remote detection of plant stresses. A cohort of pepper plants (Capsicum annuum L.) were grown 42 days at 25 °C, 70% relative humidity, and 300 μmol m-2 s-1 of photosynthetically active radiation (PAR; from 400 to 700 nm). Plants were grown under nutritional stresses induced by irrigating subsets of the plants with 100, 50, 25, or 10% of a standard nutrient solution. Reflectance spectra of the healthy and stressed plants were collected under seven artificial lamps including two tungsten halogen lamps, plus high pressure sodium, metal halide, fluorescent, microwave, and red/blue light emitting diode (LED) sources. Results indicated that several common algorithms used to estimate biomass and leaf chlorophyll content were effective in predicting plant stress under all seven illumination sources. However, the two types of tungsten halogen lamps and the microwave illumination source yielded linear models with the highest residuals and thus the highest predictive capabilities of all lamps tested. The illumination sources with the least predictive capabilities were the red/blue LEDs and fluorescent lamps. Although the red/blue LEDs yielded the lowest residuals for linear models derived from the remote sensing data, the LED arrays used in these experiments were optimized for plant productivity and not the collection of remote sensing data. Thus, we propose that if adjusted to optimize the collectio n of remote sensing information from plants, LEDs remain the best candidates for illumination sources for monitoring plant stresses in bioregenerative life support systems.

Handheld lasers allow efficient detection of fluorescent marked organisms in the field.

PubMed

Rice, Kevin B; Fleischer, Shelby J; De Moraes, Consuelo M; Mescher, Mark C; Tooker, John F; Gish, Moshe

2015-01-01

Marking organisms with fluorescent dyes and powders is a common technique used in ecological field studies that monitor movement of organisms to examine life history traits, behaviors, and population dynamics. External fluorescent marking is relatively inexpensive and can be readily employed to quickly mark large numbers of individuals; however, the ability to detect marked organisms in the field at night has been hampered by the limited detection distances provided by portable fluorescent ultraviolet lamps. In recent years, significant advances in LED lamp and laser technology have led to development of powerful, low-cost ultraviolet light sources. In this study, we evaluate the potential of these new technologies to improve detection of fluorescent-marked organisms in the field and to create new possibilities for tracking marked organisms in visually challenging environments such as tree canopies and aquatic habitats. Using handheld lasers, we document a method that provides a fivefold increase in detection distance over previously available technologies. This method allows easy scouting of tree canopies (from the ground), as well as shallow aquatic systems. This novel detection method for fluorescent-marked organisms thus promises to significantly enhance the use of fluorescent marking as a non-destructive technique for tracking organisms in natural environments, facilitating field studies that aim to document otherwise inaccessible aspects of the movement, behavior, and population dynamics of study organisms, including species with significant economic impacts or relevance for ecology and human health.

Soybean stem growth under high-pressure sodium with supplemental blue lighting

NASA Technical Reports Server (NTRS)

Wheeler, R. M.; Mackowiak, C. L.; Sager, J. C.

1991-01-01

To study high-pressure sodium (HPS) lamps used for plant lighting because of their high energy conversion efficiencies, 'McCall' soybean plants were grown for 28 days in growth chambers utilizing HPS lamps, with/without supplemental light from blue phosphor fluorescent lamps. Total photosynthetic photon flux levels, including blue fluorescent, were maintained near 300 or 500 micromol/sq m s. Results indicate that employment of HPS or other blue-deficient sources for lighting at low to moderate photosynthetic photon flux levels may cause abnormal stem elongation, but this can be prevented by the addition of a small amount of supplemental blue light.

Preliminary Results on Luminaire Designs for Hybrid Solar Lighting Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Earl, D.D.

2001-06-15

We report on the design of two hybrid lighting luminaires that blend light from a fiber optic end-emitted solar source with electric T8 fluorescent lamps. Both designs involve the retrofit of a commercially-available recessed fluorescent luminaire with minimal reductions in the original luminaire's optical efficiency. Two methods for high-angle dispersion of fiber optic end-emitted solar light are described and the resulting spatial intensity distributions, simulated using ZEMAX, are compared with standard cylindrical fluorescent tubes. Differences in spatial intensity distribution are qualitatively characterized and potential design improvements discussed.

77 FR 4203 - Energy Conservation Program: Test Procedures for General Service Fluorescent Lamps, General...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-27

...On September 14, 2011, the U.S. Department of Energy (DOE) issued a notice of proposed rulemaking (NOPR) to amend the test procedures for general service fluorescent lamps (GSFLs), general service incandescent lamps (GSILs), and incandescent reflector lamps (IRLs). That proposed rulemaking serves as the basis for today's action. DOE is amending its test procedures for GSFLs and GSILs established under the Energy Policy and Conservation Act (EPCA). DOE is not amending in this final rule the existing test procedure for IRLs established under EPCA. For GSFLs and GSILs, DOE is updating several references to the industry standards referenced in DOE's test procedures. DOE is also establishing a lamp lifetime test procedure for GSILs. These test procedures also provide the protocols upon which the Federal Trade Commission bases its energy guide label for these products. DOE's review of the GSFL, GSIL, and IRL test procedures fulfills the EPCA requirement that DOE review test procedures for all covered products at least once every seven years.

Monte Carlo treatment of resonance-radiation imprisonment in fluorescent lamps—revisited

NASA Astrophysics Data System (ADS)

Anderson, James B.

2016-12-01

We reported in 1985 a Monte Carlo treatment of the imprisonment of the 253.7 nm resonance radiation from mercury in the mercury-argon discharge of fluorescent lamps. The calculated spectra of the emitted radiation were found in good agreement with measured spectra. The addition of the isotope mercury-196 to natural mercury was found, also in agreement with experiments, to increase lamp efficiency. In this paper we report the extension of the earlier work with increased accuracy, analysis of photon exit-time distributions, recycling of energy released in quenching, analysis of dynamic similarity for different lamp sizes, variation of Mrozowski transfer rates, prediction and analysis of the hyperfine ultra-violet spectra, and optimization of tailored mercury isotope mixtures for increased lamp efficiency. The spectra were found insensitive to the extent of quenching and recycling. The optimized mixtures were found to increase efficiencies by as much as 5% for several lamp configurations. Optimization without increasing the mercury-196 fraction was found to increase efficiencies by nearly 1% for several configurations.

16 CFR 305.21 - Test data records.

Code of Federal Regulations, 2014 CFR

2014-01-01

... each basic model, or the light output, energy usage, correlated color temperature, and life ratings and, for fluorescent lamps, the color rendering index, for each basic model or lamp type were derived. [52...

Fluorescent Lamp Glass Waste Incorporation into Clay Ceramic: A Perfect Solution

NASA Astrophysics Data System (ADS)

Morais, Alline Sardinha Cordeiro; Vieira, Carlos Maurício Fontes; Rodriguez, Rubén Jesus Sanchez; Monteiro, Sergio Neves; Candido, Veronica Scarpini; Ferreira, Carlos Luiz

2016-09-01

The mandatory use of fluorescent lamps as part of a Brazilian energy-saving program generates a huge number of spent fluorescent lamps (SFLs). After operational life, SFLs cannot be disposed as common garbage owing to mercury and lead contamination. Recycling methods separate contaminated glass tubes and promote cleaning for reuse. In this work, glass from decontaminated SFLs was incorporated into clay ceramics, not only as an environmental solution for such glass wastes and clay mining reduction but also due to technical and economical advantages. Up to 30 wt.% of incorporation, a significant improvement in fired ceramic flexural strength and a decrease in water absorption was observed. A prospective analysis showed clay ceramic incorporation as an environmentally correct and technical alternative for recycling the enormous amount of SFLs disposed of in Brazil. This could also be a solution for other world clay ceramic producers, such as US, China and some European countries.

Determination of mercury distribution inside spent compact fluorescent lamps by atomic absorption spectrometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rey-Raap, Natalia; Gallardo, Antonio, E-mail: gallardo@emc.uji.es

Highlights: Black-Right-Pointing-Pointer New treatments for CFL are required considering the aim of Directive 202/96/CE. Black-Right-Pointing-Pointer It is shown that most of the mercury introduced into a CFL is in the phosphor powder. Black-Right-Pointing-Pointer Experimental conditions for microwave-assisted sample digestion followed by AAS measurements are described. Black-Right-Pointing-Pointer By washing the glass it is possible to reduce the concentration below legal limits. - Abstract: In this study, spent compact fluorescent lamps were characterized to determine the distribution of mercury. The procedure used in this research allowed mercury to be extracted in the vapor phase, from the phosphor powder, and the glass matrix.more » Mercury concentration in the three phases was determined by the method known as cold vapor atomic absorption spectrometry. Median values obtained in the study showed that a compact fluorescent lamp contained 24.52 {+-} 0.4 ppb of mercury in the vapor phase, 204.16 {+-} 8.9 ppb of mercury in the phosphor powder, and 18.74 {+-} 0.5 ppb of mercury in the glass matrix. There are differences in mercury concentration between the lamps since the year of manufacture or the hours of operation affect both mercury content and its distribution. The 85.76% of the mercury introduced into a compact fluorescent lamp becomes a component of the phosphor powder, while more than 13.66% is diffused through the glass matrix. By washing and eliminating all phosphor powder attached to the glass surface it is possible to classified the glass as a non-hazardous waste.« less

Physics of Incandescent Lamp Burnout

NASA Astrophysics Data System (ADS)

Gluck, Paul; King, John

2008-01-01

Incandescent lamps with tungsten filaments have been in use for about a century while being gradually replaced by fluorescent lamps; in another generation both will quite probably be largely replaced by light-emitting diodes. Incandescent lamps (simply called lamps in what follows) burn out after a lifetime that depends mostly on the temperature of the filament and hence the applied voltage. A full-term project (about 100 hours) on lamp burnout was carried out by two students in 1965 and has been briefly described. Many aspects of the physics of lamps have been dealt with in articles that have appeared in this journal, in the American Journal of Physics, and in Physics Education.2,3

Comparative and quantitative analysis of white light-emitting diodes and other lamps used for home illumination

NASA Astrophysics Data System (ADS)

Rubinger, Rero Marques; da Silva, Edna Raimunda; Pinto, Daniel Zaroni; Rubinger, Carla Patrícia Lacerda; Oliveira, Adhimar Flávio; da Costa Bortoni, Edson

2015-01-01

We compared the photometric and radiometric quantities in the visible, ultraviolet, and infrared spectra of white light-emitting diodes (LEDs), incandescent light bulbs and a compact fluorescent lamp used for home illumination. The color-rendering index and efficiency-related quantities were also used as auxiliary tools in this comparison. LEDs have a better performance in all aspects except for the color-rendering index, which is better with an incandescent light bulb. Compact fluorescent lamps presented results that, to our knowledge, do not justify their substitution for the incandescent light bulb. The main contribution of this work is an approach based on fundamental quantities to evaluate LEDs and other light sources.

Mercury Vapor Release from Broken Compact Fluorescent Lamps and In Situ Capture by New Nanomaterial Sorbents

PubMed Central

2008-01-01

The projected increase in the use of compact fluorescent lamps (CFLs) motivates the development of methods to manage consumer exposure to mercury and its environmental release at the end of lamp life. This work characterizes the time-resolved release of mercury vapor from broken CFLs and from underlying substrates after removal of glass fragments to simulate cleanup. In new lamps, mercury vapor is released gradually in amounts that reach 1.3 mg or 30% of the total lamp inventory after four days. Similar time profiles but smaller amounts are released from spent lamps or from underlying substrates. Nanoscale formulations of S, Se, Cu, Ni, Zn, Ag, and WS2 are evaluated for capture of Hg vapor under these conditions and compared to conventional microscale formulations. Adsorption capacities range over 7 orders of magnitude, from 0.005 (Zn micropowder) to 188 000 μg/g (unstabilized nano-Se), depending on sorbent chemistry and particle size. Nanosynthesis offers clear advantages for most sorbent chemistries. Unstabilized nano-selenium in two forms (dry powder and impregnated cloth) was successfully used in a proof-of-principle test for the in situ, real-time suppression of Hg vapor escape following CFL fracture. PMID:18754507

Mercury vapor release from broken compact fluorescent lamps and in situ capture by new nanomaterial sorbents.

PubMed

Johnson, Natalie C; Manchester, Shawn; Sarin, Love; Gao, Yuming; Kulaots, Indrek; Hurt, Robert H

2008-08-01

The projected increase in the use of compact fluorescent lamps (CFLs) motivates the development of methods to manage consumer exposure to mercury and its environmental release at the end of lamp life. This work characterizes the time-resolved release of mercury vapor from broken CFLs and from underlying substrates after removal of glass fragments to simulate cleanup. In new lamps, mercury vapor is released gradually in amounts that reach 1.3 mg or 30% of the total lamp inventory after four days. Similar time profiles but smaller amounts are released from spent lamps or from underlying substrates. Nanoscale formulations of S, Se, Cu, Ni, Zn, Ag, and WS2 are evaluated for capture of Hg vapor under these conditions and compared to conventional microscale formulations. Adsorption capacities range over 7 orders of magnitude, from 0.005 (Zn micropowder) to 188 000 microg/g (unstabilized nano-Se), depending on sorbent chemistry and particle size. Nanosynthesis offers clear advantages for most sorbent chemistries. Unstabilized nano-selenium in two forms (dry powder and impregnated cloth) was successfully used in a proof-of-principle test for the in situ, real-time suppression of Hg vapor escape following CFL fracture.

Cold starting of fluorescent lamps - part II: experiments on glow times and electrode damaging

NASA Astrophysics Data System (ADS)

Langer, Reinhard; Paul, Irina; Hilscher, Achim; Horn, Siegfried; Tidecks, Reinhard

2017-01-01

In the present work we present experiments on cold start and the resulting electrode damaging (reducing lamp life) of AC driven fluorescent lamps. The crucial parameter is the glow time, determined from time resolved measurements of lamp voltage and current. The relation between the energy consumed during glow phase and the glow time is studied. It turns out that there is no common threshold of energy until the glow-to-arc transition takes place, but strong energy input into the lamp yields short glow times. The transient behaviour from the glow to the arc regime is investigated and the stable operation points of the arc discharge are determined, yielding an arc discharge voltage-current characteristics of the lamp type investigated. The electrode damage is investigated as a function of the open source voltage and the ballast resistance. Subsequent cold starts lead to an increase of the glow time due to electrode damaging, i.e., the electrode damage accumulates. Different regeneration procedures and their effectiveness are compared. Regeneration burning turns out to be more effective than heating up the electrode. A criterion for avoiding high electrode damage is obtained, indicating that the average power during glow time should exceed 20 W.

Handheld Lasers Allow Efficient Detection of Fluorescent Marked Organisms in the Field

PubMed Central

Fleischer, Shelby J.; De Moraes, Consuelo M.; Mescher, Mark C.; Tooker, John F.

2015-01-01

Marking organisms with fluorescent dyes and powders is a common technique used in ecological field studies that monitor movement of organisms to examine life history traits, behaviors, and population dynamics. External fluorescent marking is relatively inexpensive and can be readily employed to quickly mark large numbers of individuals; however, the ability to detect marked organisms in the field at night has been hampered by the limited detection distances provided by portable fluorescent ultraviolet lamps. In recent years, significant advances in LED lamp and laser technology have led to development of powerful, low-cost ultraviolet light sources. In this study, we evaluate the potential of these new technologies to improve detection of fluorescent-marked organisms in the field and to create new possibilities for tracking marked organisms in visually challenging environments such as tree canopies and aquatic habitats. Using handheld lasers, we document a method that provides a fivefold increase in detection distance over previously available technologies. This method allows easy scouting of tree canopies (from the ground), as well as shallow aquatic systems. This novel detection method for fluorescent-marked organisms thus promises to significantly enhance the use of fluorescent marking as a non-destructive technique for tracking organisms in natural environments, facilitating field studies that aim to document otherwise inaccessible aspects of the movement, behavior, and population dynamics of study organisms, including species with significant economic impacts or relevance for ecology and human health. PMID:26035303

Biomolecular and structural analyses of cauliflower-like DNAs by ultraviolet, circular dichroism, and fluorescence spectroscopies in comparison with natural DNA.

PubMed

Gill, Pooria; Ranjbar, Bijan; Saber, Reza; Khajeh, Khosro; Mohammadian, Mehdi

2011-07-01

Cauliflower-like DNAs are stem-loop DNAs that are fabricated periodically in inverted repetitions from deoxyribonucleic acid phosphates (dNTPs) by loop-mediated isothermal amplification (LAMP). Cauliflower-like DNAs have ladder-shape behaviors on gel electrophoresis, and increasing the time of LAMP leads to multiplying the repetitions, stem-loops, and electrophoretic bands. Cauliflower-like DNAs were fabricated via LAMP using two loop primers, two bumper primers, dNTPs, a λ-phage DNA template, and a Bst DNA polymerase in 75- and 90-min periods. These times led to manufacturing two types of cauliflower-like DNAs with different contents of inverted repetitions and stem-loops, which were clearly indicated by two comparable electrophoresis patterns in agarose gel. LAMP-fabricated DNAs and natural dsB-DNA (salmon genomic DNA) were dialyzed in Gomori phosphate buffer (10 mM, pH 7.4) to be isolated from salts, nucleotides, and primers. Dialyzed DNAs were studied using UV spectroscopy, circular dichroism spectropolarimetry, and fluorescence spectrophotometry. Structural analyses indicated reduction of the molecular ellipticity and extinction coefficients in comparison with B-DNA. Also, cauliflower-like DNAs demonstrated less intrinsic and more extrinsic fluorescence in comparison with natural DNA. The overwinding and lengthening of the cauliflower-like configurations of LAMP DNAs led to changes in physical parameters of this type of DNA in comparison with natural DNA. The results obtained introduced new biomolecular characteristics of DNA macromolecules fabricated within a LAMP process and show the effects of more inverted repeats and stem-loops, which are manufactured by lengthening the process.

Instantaneous and efficient surface wave excitation of a low pressure gas or gases

DOEpatents

Levy, Donald J.; Berman, Samuel M.

1988-01-01

A system for instantaneously ionizing and continuously delivering energy in the form of surface waves to a low pressure gas or mixture of low pressure gases, comprising a source of rf energy, a discharge container, (such as a fluorescent lamp discharge tube), an rf shield, and a coupling device responsive to rf energy from the source to couple rf energy directly and efficiently to the gas or mixture of gases to ionize at least a portion of the gas or gases and to provide energy to the gas or gases in the form of surface waves. The majority of the rf power is transferred to the gas or gases near the inner surface of the discharge container to efficiently transfer rf energy as excitation energy for at least one of the gases. The most important use of the invention is to provide more efficient fluorescent and/or ultraviolet lamps.

40 CFR Appendix D to Part 403 - Selected Industrial Subcategories Considered Dilute for Purposes of the Combined Wastestream Formula

Code of Federal Regulations, 2012 CFR

2012-07-01

... (40 CFR parts 413/433). Capacitors (Fluid Fill) Carbon and Graphite Products Dry Transformers Ferrite Electronic Devices Fixed Capacitors Fluorescent Lamps Fuel Cells Incandescent Lamps Magnetic Coatings Mica...

Occupational exposure in the fluorescent lamp recycling sector in France

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zimmermann, François, E-mail: francois.zimmermann@inrs.fr; Lecler, Marie-Thérèse; Clerc, Frédéric

Highlights: • Chemical risks were assessed in the five fluorescent lamp recycling facilities. • The main hazardous agents are mercury vapors and dust containing lead and yttrium. • Exposure and pollutant levels were correlated with steps and processes. • All the stages and processes are concerned by worrying levels of pollutants. • We suggest recommendations to reduce chemical risk. - Abstract: The fluorescent lamp recycling sector is growing considerably in Europe due to increasingly strict regulations aimed at inciting the consumption of low energy light bulbs and their end-of-life management. Chemical risks were assessed in fluorescent lamp recycling facilities bymore » field measurement surveys in France, highlighting that occupational exposure and pollutant levels in the working environment were correlated with the main recycling steps and processes. The mean levels of worker exposure are 4.4 mg/m{sup 3}, 15.4 μg/m{sup 3}, 14.0 μg/m{sup 3}, 247.6 μg/m{sup 3}, respectively, for total inhalable dust, mercury, lead and yttrium. The mean levels of airborne pollutants are 3.1 mg/m{sup 3}, 9.0 μg/m{sup 3}, 9.0 μg/m{sup 3}, 219.2 μg/m{sup 3}, respectively, for total inhalable dust, mercury, lead and yttrium. The ranges are very wide. Surface samples from employees’ skin and granulometric analysis were also carried out. The overview shows that all the stages and processes involved in lamp recycling are concerned by the risk of hazardous substances penetrating into the bodies of employees, although exposure of the latter varies depending on the processes and tasks they perform. The conclusion of this study strongly recommends the development of a new generation of processes in parallel with more information sharing and regulatory measures.« less

LAMP2GO

DOE Office of Scientific and Technical Information (OSTI.GOV)

Priye, Aashish

2017-06-08

The app is a unique image analysis software and acts as a fluorescence reader for multiplexed nucleic acid amplification reactions. If the reaction is positive, a bright fluorescent signal is emitted from the solution depending on the choice of fluorophore molecule. The app reads the Red, blue and green (RGB) channels of the emitted signal and transforms it to yield the chromaticity (x and y) and luminance of the signal. This new representation of signal is far superior to the RGB system in determining the signal color and intensity.

Refractive errors among students occupying rooms lighted with incandescent or fluorescent lamps.

PubMed

Czepita, Damian; Gosławski, Wojciech; Mojsa, Artur

2004-01-01

The purpose of the study was to determine whether the development of refractive errors could be associated with exposure to light emitted by incandescent or fluorescent lamps. 3636 students were examined (1638 boys and 1998 girls, aged 6-18 years, mean age 12.1, SD 3.4). The examination included retinoscopy with cycloplegia. Myopia was defined as refractive error < or = -0.5 D, hyperopia as refractive error > or = +1.5 D, astigmatism as refractive error > 0.5 DC. Anisometropia was diagnosed when the difference in the refraction of both eyes was > 1.0 D. The children and their parents completed a questionnaire on exposure to light at home. Data were analyzed statistically with the chi2 test. P values of less than 0.05 were considered statistically significant. It was found that the use of fluorescent lamps was associated with an increase in the occurrence of hyperopia (P < 0.01). There was no association between sleeping with the light turned on and prevalence of refractive errors.

16 CFR 305.6 - Sampling.

Code of Federal Regulations, 2012 CFR

2012-01-01

... REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ) Testing § 305.6 Sampling. (a) For any covered product (except general service fluorescent lamps or general service lamps), any representation...

16 CFR 305.6 - Sampling.

Code of Federal Regulations, 2013 CFR

2013-01-01

... REGARDING ENERGY CONSUMPTION AND WATER USE OF CERTAIN HOME APPLIANCES AND OTHER PRODUCTS REQUIRED UNDER THE ENERGY POLICY AND CONSERVATION ACT (âAPPLIANCE LABELING RULEâ) Testing § 305.6 Sampling. (a) For any covered product (except general service fluorescent lamps or general service lamps), any representation...

Improved high-intensity microwave discharge lamp for atomic resonance absorption and fluorescence spectrometry.

PubMed

Lifshitz, A; Skinner, G B; Wood, D R

1978-09-01

An unusually good combination of high intensity and narrow line has been achieved in a microwave discharge lamp by placing the optical window in the center of the microwave cavity. Construction details and performance characteristics are described.

Evaluation of a LED-based flatbed document scanner for radiochromic film dosimetry in transmission mode.

PubMed

Lárraga-Gutiérrez, José Manuel; García-Garduño, Olivia Amanda; Treviño-Palacios, Carlos; Herrera-González, José Alfredo

2018-03-01

Flatbed scanners are the most frequently used reading instrument for radiochromic film dosimetry because its low cost, high spatial resolution, among other advantages. These scanners use a fluorescent lamp and a CCD array as light source and detector, respectively. Recently, manufacturers of flatbed scanners replaced the fluorescent lamp by light emission diodes (LED) as a light source. The goal of this work is to evaluate the performance of a commercial flatbed scanner with LED based source light for radiochromic film dosimetry. Film read out consistency, response uniformity, film-scanner sensitivity, long term stability and total dose uncertainty was evaluated. In overall, the performance of the LED flatbed scanner is comparable to that of a cold cathode fluorescent lamp (CCFL). There are important spectral differences between LED and CCFL lamps that results in a higher sensitivity of the LED scanner in the green channel. Total dose uncertainty, film response reproducibility and long-term stability of LED scanner are slightly better than those of the CCFL. However, the LED based scanner has a strong non-uniform response, up to 9%, that must be adequately corrected for radiotherapy dosimetry QA. The differences in light emission spectra between LED and CCFL lamps and its potential impact on film-scanner sensitivity suggest that the design of a dedicated flat-bed scanner with LEDs may improve sensitivity and dose uncertainty in radiochromic film dosimetry. Copyright © 2018 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. All rights reserved.

Studies on cytostatics used as photosensitizing material in photodynamic therapy

NASA Astrophysics Data System (ADS)

Pascu, Mihail-Lucian; Danaila, Leon; Carstocea, Benone D.; Staicu, Angela; Truica, Sorina; Gazdaru, Doina M.

2002-10-01

Introduction of the photosensitizer properties of cytostatics drus was made, pointing out that the fact that besides the biochemical action of the cytostatics their effects could be enhanced by the exposure to light at different doses. A spectroscopical characterisation of methotrexate and fluorouracil, cytostatic drugs used frequently in cancer therpy was performed. The absorption, emission and excitation spectra were measured for methotrexate solutions in natural saline and sodium hydroxide at concentration in the range 10-5 -10-6M and pH 8.4. The absorption, emission and excitation spectra were measured for fluorouracil solutions in natural saline at concentration in the range 10-4 -10-5M. The absorption spectrum exhibits spectral bands in the range 250nm -450nm for both drugs. The fluorescence excitatioan for methotrexate was made at 340nm and 370nm, the fluorescence emission was detected in the spectral range 400nm - 500nm with a maximum at 470nm. The fluorescence excitation was measured in teh range 200nm-500nm with the emission centred on 530nm, for Xe lamp irradiation, and 300nm for Hg lamp and laser irradiation. The fluorescence emission spectra was monitored in the spectral range 400nm - 600nm. The effects of irradiation on spectroscopic characteristics of methrotrexate and fluorouracil were investigated. The irraditaion was made using a UV classic lamp with Xe, for the first experimental part and for the second one it was used both a class Hg lamp and a nytorgen pulsed laser.

Billion shot flashlamp for spaceborne lasers

NASA Technical Reports Server (NTRS)

Richter, Linda; Schuda, Felix; Degnan, John

1990-01-01

A billion-shot flashlamp developed under a NASA contract for spaceborne laser missions is presented. Lifetime-limiting mechanisms are identified and addressed. Two energy loadings of 15 and 44 Joules were selected for the initial accelerated life testing. A fluorescence-efficiency test station was used for measuring the useful-light output degradation of the lamps. The design characteristics meeting NASA specifications are outlined. Attention is focused on the physical properties of tungsten-matrix cathodes, the chemistry of dispenser cathodes, and anode degradation. It is reported that out of the total 83 lamps tested in the program, 4 lamps reached a billion shots and one lamp is beyond 1.7 billion shots, while at 44 Joules, 4 lamps went beyond 100 million shots and one lamp reached 500 million shots.

Lighting for Education.

ERIC Educational Resources Information Center

Ontario Ministry of Colleges and Universities, Toronto.

Some of the qualities and quantities that must be juggled to produce good lighting for educational facilities are analyzed with photographs, tables, and drawings. The three categories of lamps used for school lighting (incandescent, fluorescent, and high intensity discharge) are described; a lamp selection guide gives the design characteristics of…

Development of a quantitative loop-mediated isothermal amplification assay for the field detection of Erysiphe necator.

PubMed

Thiessen, Lindsey D; Neill, Tara M; Mahaffee, Walter F

2018-01-01

Plant pathogen detection systems have been useful tools to monitor inoculum presence and initiate management schedules. More recently, a loop-mediated isothermal amplification (LAMP) assay was successfully designed for field use in the grape powdery mildew pathosystem; however, false negatives or false positives were prevalent in grower-conducted assays due to the difficulty in perceiving the magnesium pyrophosphate precipitate at low DNA concentrations. A quantitative LAMP (qLAMP) assay using a fluorescence resonance energy transfer-based probe was assessed by grape growers in the Willamette Valley of Oregon. Custom impaction spore samplers were placed at a research vineyard and six commercial vineyard locations, and were tested bi-weekly by the lab and by growers. Grower-conducted qLAMP assays used a beta-version of the Smart-DART handheld LAMP reaction devices (Diagenetix, Inc., Honolulu, HI, USA), connected to Android 4.4 enabled, Bluetooth-capable Nexus 7 tablets for output. Quantification by a quantitative PCR assay was assumed correct to compare the lab and grower qLAMP assay quantification. Growers were able to conduct and interpret qLAMP results; however, the Erysiphe necator inoculum quantification was unreliable using the beta-Smart-DART devices. The qLAMP assay developed was sensitive to one spore in early testing of the assay, but decreased to >20 spores by the end of the trial. The qLAMP assay is not likely a suitable management tool for grape powdery mildew due to losses in sensitivity and decreasing costs and portability for other, more reliable molecular tools.

A Nano-Selenium Reactive Barrier Approach for Managing Mercury over the Life-Cycle of Compact Fluorescent Lamps

PubMed Central

Lee, Brian; Sarin, Love; Johnson, Natalie C.; Hurt, Robert H.

2013-01-01

Compact fluorescent lamps contain small quantities of mercury, whose release can lead to human exposures of potential concern in special cases involving multiple lamps, confined spaces, or young children. The exposure scenarios typically involve solid lamp debris that slowly releases elemental mercury vapor to indoor spaces. Here we propose and demonstrate a reactive barrier approach for the suppression of that mercury release, and demonstrate the concept using uncoated amorphous nano-selenium as the reactive component. Multi-layer structures containing an impregnated reactive layer and a mercury vapor barrier are fabricated, characterized, and evaluated in three exposure prevention scenarios: carpeted break sites, disposal/recycling bags, and boxes as used for retail sales, shipping and collection. The reactive barriers achieve significant suppression of mercury release to indoor spaces in each of the three scenarios. The nano-selenium barriers also exhibit a unique indicator function that can reveal the location of Hg-contamination by local reaction-induced change in optical properties. The article also presents results on equilibrium Hg vapor pressure above lamp debris, mathematical modeling of reaction and transport processes within reactive barriers, and landfill stability of nano-selenium and its reaction products. PMID:19731697

A nano-selenium reactive barrier approach for managing mercury over the life-cycle of compact fluorescent lamps.

PubMed

Lee, Brian; Sarin, Love; Johnson, Natalie C; Hurt, Robert H

2009-08-01

Compact fluorescent lamps contain small quantities of mercury, release of which can lead to human exposures of potential concern in special cases involving multiple lamps, confined spaces, or young children. The exposure scenarios typically involve solid lamp debris that slowly releases elemental mercury vapor to indoor spaces. Here we propose and demonstrate a reactive barrier approach for the suppression of that mercury release, and demonstrate the concept using uncoated amorphous nanoselenium as the reactive component. Multilayer structures containing an impregnated reactive layer and a mercury vapor barrier are fabricated, characterized, and evaluated in three exposure prevention scenarios: carpeted break sites, disposal/recycling bags, and boxes as used for retail sales, shipping, and collection. The reactive barriers achieve significant suppression of mercury release to indoor spaces in each of thethree scenarios. The nanoselenium barriers also exhibit a unique indicator function that can reveal the location of Hg contamination by local reaction-induced change in optical properties. The article also presents results on equilibrium Hg vapor pressure above lamp debris, mathematical modeling of reaction and transport processes within reactive barriers, and landfill stability of nanoselenium and its reaction products.

Polarized fluorescence for skin cancer diagnostic with a multi-aperture camera

NASA Astrophysics Data System (ADS)

Kandimalla, Haripriya; Ramella-Roman, Jessica C.

2008-02-01

Polarized fluorescence has shown some promising results in assessment of skin cancer margins. Researchers have used tetracycline and cross polarization imaging for nonmelanoma skin cancer demarcation as well as investigating endogenous skin polarized fluorescence. In this paper we present a new instrument for polarized fluorescence imaging, able to calculate the full fluorescence Stokes vector in one snapshot. The core of our system is a multi-aperture camera constructed with a two by two lenslet array. Three of the lenses have polarizing elements in front of them, oriented at 0°, + 45°and 90° with respect to light source polarization. A flash lamp combined with a polarizer parallel to the source-camera-sample plane and a UV filter is used as an excitation source. A blue filter in front of the camera system is used to collect only the fluorescent emission of interest and filter out the incident light. In-vitro tests of endogenous and exogenous polarized fluorescence on collagen rich material like bovine tendon were performed and Stokes vector of polarized fluorescence calculated. The system has the advantage of eliminating moving artifacts with the collection of different polarization states and stoke vector in a single snap shot.

Thermally stable green Ba(3)Y(PO(4))3:Ce(3+),Tb(3+) and red Ca(3)Y(AlO)(3)(BO(3))4:Eu(3+) phosphors for white-light fluorescent lamps.

PubMed

Huang, Chien-Hao; Kuo, Te-Wen; Chen, Teng-Ming

2011-01-03

A class of thermal stable of green-emitting phosphors Ba(3)Y(PO(4))(3):Ce(3+),Tb(3+) (BYP:Ce(3+),Tb(3+)) and red-emitting phosphors Ca(3)Y(AlO)(3)(BO(3))(4):Eu(3+) (CYAB:Eu(3+)) for white-light fluorescent lamps were synthesized by high temperature solid-state reaction. We observed a decay of only 3% at 150 °C for BYP:0.25Ce3+,0.25Tb3+ (3% for LaPO4:Ce(3+),Tb(3+)), and a decay of 4% for CYAB:0.5Eu(3+) (7% for Y(2)O(3):Eu(3+), 24% for Y(2)O(2)S:Eu(3+)). The emission intensity of composition-optimized Ba(3)(Y(0.5)Ce(0.25)Tb(0.25))(PO(4))(3) is 70% of that of commercial LaPO(4):Ce(3+),Tb(3+) phosphors, and the CIE chromaticity coordinates are found to be (0.323, 0.534). The emission intensity of Ca(3)(Y(0.5)Eu(0.5))(AlO)(3)(BO(3))(4) is 70% and 83% of those of Y(2)O(3):Eu(3+) and Y(2)O(2)S:Eu(3+) phosphors, respectively, and the CIE chromaticity coordinates are redder (0.652, 0.342) than those of Y(2)O(3):Eu(3+) (0.645, 0.347) and Y(2)O(2)S:Eu(3+) (0.647, 0.343). A white-light fluorescent lamp is fabricated using composition-optimized Ba(3)(Y(0.5)Ce(0.25)Tb(0.25))(PO(4))(3) and Ca(3)(Y(0.5)Eu(0.5))(AlO)(3)(BO(3))(4) phosphors and matching blue-emitting phosphors. The results indicate that the quality of the brightness and color reproduction is suitable for application in shortwave UV fluorescent lamps. The white-light fluorescent lamp displays CIE chromaticity coordinates of x = 0.33, y = 0.35, a warm white light with a correlated color temperature of 5646 K, and a color-rendering index of Ra = 70.

Growing root, tuber and nut crops hydroponically for CELSS

NASA Technical Reports Server (NTRS)

Hill, W. A.; Mortley, D. G.; Loretan, P. A.; Bonsi, C. K.; Morris, C. E.; Mackowiak, C. L.; Wheeler, R. M.; Tibbitts, T. W.

1992-01-01

Among the crops selected by NASA for growth in controlled ecological life-support systems are four that have subsurface edible parts: potatoes, sweet potatoes, sugar beets and peanuts. These crops can be produced in open and closed (recirculating), solid media and liquid, hydroponic systems. Fluorescent, fluorescent plus incandescent, and high-pressure sodium-plus-metal-halide lamps have proven to be effective light sources. Continuous light with 16-C and 28/22-C (day/night) temperatures produce highest yields for potato and sweet potato, respectively. Dry weight yields of up to 4685, 2541, 1151 and 207 g/sq m for potatoes, sweet potatoes, sugar beets and peanuts, respectively, are produced in controlled environment hydroponic systems.

75 FR 37975 - Energy Conservation Program for Consumer Products and Certain Commercial and Industrial Equipment...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-01

... technologies including induction and fluorescent lamps. 75 FR 22031, 22033 (April 27, 2010). In comments... of magnetic ballasts of 50,000 hours. After the 2003 draft report, Allegheny noted that MV ballast... considered only magnetic ballasts because they are the most common ballast used in HID lighting systems. DOE...

The fate and management of high mercury-containing lamps from high technology industry.

PubMed

Chang, T C; You, S J; Yu, B S; Kong, H W

2007-03-22

This study investigated the fate and management of high mercury-contained lamps, such as cold cathode fluorescent lamps (CCFLs), ultraviolet lamps (UV lamps), and super high pressure mercury lamps (SHPs), from high technology industries in Taiwan, using material flow analysis (MFA) method. Several organizations, such as Taiwan Environmental Protection Administration, Taiwan External Trade Development Council, the light sources manufactories, mercury-containing lamps importer, high technology industrial user, and waste mercury-containing lamps treatment facilities were interviewed in this study. According to this survey, the total mercury contained in CCFLs, UV lamps, and SHPs produced in Taiwan or imported from other countries was 886kg in year 2004. Among the various lamps containing mercury, 57kg mercury was exported as primary CCFLs, 7kg mercury was wasted as defective CCFLs, and 820kg mercury was used in the high technology industries, including 463kg mercury contained in exported industrial products using CCFLs as components. On the contrary, only 59kg of mercury was exported, including 57kg in CCFLs and 2kg in UV lamps. It reveals that 364kg mercury was consumed in Taiwan during year 2004. In addition, 140kg of the 364kg mercury contained in lamps used by high technology industry was well treated through industrial waste treatment system. Among the waste mercury from high technology industry, 80kg (57%), 53kg (38%), and 7kg (5%) of mercury were through domestic treatment, offshore treatment, and emission in air, respectively. Unfortunately, 224kg waste mercury was not suitable treated, including 199kg mercury contained in CCFL, which is a component of monitor for personal computer and liquid crystal display television, and 25kg non-treated mercury. Thus, how to recover the mercury from the waste monitors is an important challenge of zero wastage policy in Taiwan.

The injury and cumulative effects on human skin by UV exposure from artificial fluorescence emission.

PubMed

Tian, Yan; Liu, Wei; Niu, TianHui; Dai, CaiHong; Li, Xiaoxin; Cui, Caijuan; Zhao, Xinyan; E, Yaping; Lu, Hui

2014-01-01

The injury and cumulative effects of UV emission from fluorescence lamp were studied. UV intensity from fluorescence lamp was measured, and human skin samples (hips, 10 volunteers) were exposed to low-dose UV irradiation (three times per week for 13 consecutive weeks). Three groups were examined: control group without UV radiation; low-dose group with a cumulative dose of 50 J cm(-2) which was equivalent to irradiation of the face during indoor work for 1.5 years; and high-dose group with 1000 J cm(-2) cumulative dose equivalent to irradiation of the face during outdoor activities for 1 year. Specific indicators were measured before and after UVA irradiation. The findings showed that extending the low-dose UVA exposure decreased the skin moisture content and increased the transepidermal water loss as well as induced skin color changes (decreased L* value, increased M index). Furthermore, irradiated skin showed an increased thickness of cuticle and epidermis, skin edema, light color and unclear staining collagen fibers in the dermis, and elastic fiber fragmentation. In addition, MMP-1, p53 and SIRT1 expression was also increased. Long-term exposure of low-dose UVA radiation enhanced skin photoaging. The safety of the fluorescent lamp needs our attention. © 2014 The American Society of Photobiology.

On the Intensity Profile of Electric Lamps and Light Bulbs

ERIC Educational Resources Information Center

Bacalla, Xavier; Salumbides, Edcel John

2013-01-01

We demonstrate that the time profile of the light intensity from domestic lighting sources exhibits simple yet interesting properties that foster lively student discussions. We monitor the light intensity of an industrial fluorescent lamp (also known as TL) and an incandescent bulb using a photodetector connected to an oscilloscope. The light…

Reducing Barriers To The Use of High-Efficiency Lighting Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peter Morante

2005-12-31

With funding from the U.S. Department of Energy (DOE), the Lighting Research Center (LRC) at Rensselaer Polytechnic Institute completed the four-year research project, Reducing Barriers to the Use of High-Efficiency Lighting Systems. The initial objectives were: (1) identifying barriers to widespread penetration of lighting controls in commercial/industrial (C/I) applications that employ fluorescent lamp technologies, and (2) making recommendations to overcome these barriers. The addition of a fourth year expanded the original project objectives to include an examination of the impact on fluorescent lamps from dimming utilizing different lamp electrode heating and dimming ratios. The scope of the project was narrowedmore » to identify barriers to the penetration of lighting controls into commercial-industrial (C/I) applications that employ fluorescent lamp technologies, and to recommend means for overcoming these barriers. Working with lighting manufacturers, specifiers, and installers, the project identified technological and marketing barriers to the widespread use of lighting controls, specifically automatic-off controls, occupancy sensors, photosensors, dimming systems, communication protocols and load-shedding ballasts. The primary barriers identified include cost effectiveness of lighting controls to the building owner, lack of standard communication protocols to allow different part of the control system to communicate effectively, and installation and commissioning issues. Overcoming the identified barriers requires lighting control products on the market to achieve three main goals: (1) Achieve sufficient functionality to meet the key requirements of their main market. (2) Allow significant cost reduction compared to current market standard systems. Cost should consider: hardware capital cost including wiring, design time required by the specifier and the control system manufacturer, installation time required by the electrician, and commissioning time and remedial time required by the electrician and end user. (3) Minimize ongoing perceived overhead costs and inconvenience to the end user, or in other words, systems should be simple to understand and use. In addition, we believe that no lighting controls solution is effective or acceptable unless it contributes to, or does not compromise, the following goals: (1) Productivity--Planning, installation, commissioning, maintenance, and use of controls should not decrease business productivity; (2) Energy savings--Lighting controls should save significant amounts of energy and money in relation to the expense involved in using them (acceptable payback period); and/or (3) Reduced power demand--Society as a whole should benefit from the lowered demand for expensive power and for more natural resources. Discussions of technology barriers and developments are insufficient by themselves to achieve higher penetration of lighting controls in the market place. Technology transfer efforts must play a key role in gaining market acceptance. The LRC developed a technology transfer model to better understand what actions are required and by whom to move any technology toward full market acceptance.« less

Development of Fluorescent Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) using Quenching Probes for the Detection of the Middle East Respiratory Syndrome Coronavirus.

PubMed

Shirato, Kazuya; Semba, Shohei; El-Kafrawy, Sherif A; Hassan, Ahmed M; Tolah, Ahmed M; Takayama, Ikuyo; Kageyama, Tsutomu; Notomi, Tsugunori; Kamitani, Wataru; Matsuyama, Shutoku; Azhar, Esam Ibraheem

2018-05-12

Clinical detection of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) in patients is achieved using genetic diagnostic methods, such as real-time RT-PCR assay. Previously, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of MERS-CoV [Virol J. 2014. 11:139]. Generally, amplification of RT-LAMP is monitored by the turbidity induced by precipitation of magnesium pyrophosphate with newly synthesized DNA. However, this mechanism cannot completely exclude the possibility of unexpected reactions. Therefore, in this study, fluorescent RT-LAMP assays using quenching probes (QProbes) were developed specifically to monitor only primer-derived signals. Two primer sets (targeting nucleocapsid and ORF1a sequences) were constructed to confirm MERS cases by RT-LAMP assay only. Our data indicate that both primer sets were capable of detecting MERS-CoV RNA to the same level as existing genetic diagnostic methods, and that both were highly specific with no cross-reactivity observed with other respiratory viruses. These primer sets were highly efficient in amplifying target sequences derived from different MERS-CoV strains, including camel MERS-CoV. In addition, the detection efficacy of QProbe RT-LAMP was comparable to that of real-time RT-PCR assay using clinical specimens from patients in Saudi Arabia. Altogether, these results indicate that QProbe RT-LAMP assays described here can be used as powerful diagnostic tools for rapid detection and surveillance of MERS-CoV infections. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Flash lamp-excited time-resolved fluorescence microscope suppresses autofluorescence in water concentrates to deliver an 11-fold increase in signal-to-noise ratio.

PubMed

Connally, Russell; Veal, Duncan; Piper, James

2004-01-01

The ubiquity of naturally fluorescing components (autofluorophores) encountered in most biological samples hinders the detection and identification of labeled targets through fluorescence-based techniques. Time-resolved fluorescence (TRF) is a technique by which the effects of autofluorescence are reduced by using specific fluorescent labels with long fluorescence lifetimes (compared with autofluorophores) in conjunction with time-gated detection. A time-resolved fluorescence microscope (TRFM) is described that is based on a standard epifluorescence microscope modified by the addition of a pulsed excitation source and an image-intensified time-gateable CCD camera. The choice of pulsed excitation source for TRFM has a large impact on the price and performance of the instrument. A flash lamp with rapid discharge characteristics was selected for our instrument because of the high spectral energy in the UV region and short pulse length. However, the flash output decayed with an approximate lifetime of 18 micros and the TRFM required a long-lived lanthanide chelate label to ensure that probe fluorescence was visible after decay of the flash plasma. We synthesized a recently reported fluorescent chelate (BHHCT) and conjugated it to a monoclonal antibody directed against the waterborne parasite Giardia lamblia. For a 600-nm bandpass filter set and a gate delay of 60 micros, the TRFM provided an 11.3-fold improvement in the signal-to-noise ratio (S/N) of labeled Giardia over background. A smaller gain in an SNR of 9.69-fold was achieved with a 420-nm longpass filter set; however, the final contrast ratio between labeled cyst and background was higher (11.3 versus 8.5). Despite the decay characteristics of the light pulse, flash lamps have many practical advantages compared with optical chopper wheels and modulated lasers for applications in TRFM.

Simplified Real-Time Multiplex Detection of Loop-Mediated Isothermal Amplification Using Novel Mediator Displacement Probes with Universal Reporters.

PubMed

Becherer, Lisa; Bakheit, Mohammed; Frischmann, Sieghard; Stinco, Silvina; Borst, Nadine; Zengerle, Roland; von Stetten, Felix

2018-04-03

A variety of real-time detection techniques for loop-mediated isothermal amplification (LAMP) based on the change in fluorescence intensity during DNA amplification enable simultaneous detection of multiple targets. However, these techniques depend on fluorogenic probes containing target-specific sequences. That complicates the adaption to different targets leading to time-consuming assay optimization. Here, we present the first universal real-time detection technique for multiplex LAMP. The novel approach allows simple assay design and is easy to implement for various targets. The innovation features a mediator displacement probe and a universal reporter. During amplification of target DNA the mediator is displaced from the mediator displacement probe. Then it hybridizes to the reporter generating a fluorescence signal. The novel mediator displacement (MD) detection was validated against state-of-the-art molecular beacon (MB) detection by means of a HIV-1 RT-LAMP: MD surpassed MB detection by accelerated probe design (MD: 10 min, MB: 3-4 h), shorter times to positive (MD 4.1 ± 0.1 min shorter than MB, n = 36), improved signal-to-noise fluorescence ratio (MD: 5.9 ± 0.4, MB: 2.7 ± 0.4; n = 15), and showed equally good or better analytical performance parameters. The usability of one universal mediator-reporter set in different multiplex assays was successfully demonstrated for a biplex RT-LAMP of HIV-1 and HTLV-1 and a biplex LAMP of Haemophilus ducreyi and Treponema pallidum, both showing good correlation between target concentration and time to positive. Due to its simple implementation it is suggested to extend the use of the universal mediator-reporter sets to the detection of various other diagnostic panels.

Growing root, tuber and nut crops hydroponically for CELSS.

PubMed

Hill, W A; Mortley, D G; Mackowiak, C L; Loretan, P A; Tibbitts, T W; Wheeler, R M; Bonsi, C K; Morris, C E

1992-01-01

Among the crops selected by the National Aeronautics and Space Administration for growth in controlled ecological life support systems are four that have subsurface edible parts -- potatoes, sweet potatoes, sugar beets and peanuts. These crops have been produced in open and closed (recirculating), solid media and liquid, hydroponic systems. Fluorescent , fluorescent plus incandescent and high pressure sodium plus metal halide lamps have proven to be effective light sources. Continuous light with 16 degrees C and 28/22 degrees C (day/night) temperatures have produced highest yields for potato and sweet potato, respectively. Dry weight yields of up to 4685, 2541, 1151 and 207 g m-2 for for potatoes, sweet potatoes, sugar beets and peanuts, respectively, have been produced in controlled environment hydroponic systems.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Clark, Terry

The biggest challenge affecting lighting decisions today is future-proofing. Choices made today will likely be choices that impact the next 20 or perhaps even 40 years. For example, we can expect the best LED lighting available today to maintain 90% of initial light output for 100,000 hours or more. Compare this to a fluorescent lighting system that needs regular maintenance after only 30,000 hours due to lamp burn-outs. The simple act of choosing a LED system over a fluorescent one alone can begin to reduce life cycle costs by saving future maintenance costs. However, the choice of LED over fluorescentmore » is just the beginning. Here, let’s look at other future-proofing decisions that should be considered when choosing your next lighting system.« less

Recent Advances in Lighting Science

NASA Astrophysics Data System (ADS)

Lapatovich, Walter P.

2004-10-01

Lighting is a global industry supplying a wide array of devices and systems that emit light ranging from incandescent lamps to light emitting diodes to electric discharge lamps. Electric discharge lamps are the most familiar plasma devices to most people. This work focuses on plasma light sources, some advances in this area and recent trends. Plasma light sources fall into two broad categories, namely low pressure and high pressure. The low-pressure lamps operate in the range of 40 to 500 Pa while the high-pressure lamps operate in the range of 0.1 to 15 MPa. The corresponding electron temperatures are about 1eV and 0.5 eV for the low and high-pressure lamps respectively. High-pressure lamps are treated under the assumption of local thermodynamic equilibrium wherein the gas temperature is equilibrated with the electron temperature. They are often called high intensity discharge lamps because of their intrinsically high radiance. Within these two broad categories are many subgroups, perhaps the most important being mercury and non-mercury containing lamps. An example of a low pressure, mercury-containing lamp is the ubiquitous fluorescent lamp. Attempts to improve the efficiency of these lamps center around inductive excitation techniques and two-photon phosphor development. The plasma research on mercury-free low-pressure lamps is focused on finding substitutes for a mercury-rare gas discharge. Several ultraviolet emitting candidates have been explored which emit both UV and visible. Longer wavelength UV is of interest because of the parallel development of phosphors mated with LED excitation wavelengths around 380nm. Several examples will be discussed. There have been major advances in high intensity discharge lamps with and without mercury. Mercury containing metal halide lamps are now being fabricated from translucent ceramic envelopes instead of the conventional vitreous silica. The higher temperature tolerant envelope materials permit using discharges in vapors hitherto unacceptable because of chemical reactions. Temperature driven chemical reactions (which affect lamp life, starting and stability) are better understood. Lamps are better designed with finite element thermal modeling and thermodynamic computational tools. Improved understanding of molecular processes in the energy transport within the plasma has opened possibilities for new types of light sources relying heavily on molecular emission. Examples of lamps containing sulfur, indium, thallium and rare earth halides will be discussed. General trends in plasma based light source have been towards lower wattage, directed visible output, high quality visible output, longer life and mercury-free lamps. Consumer demand for high tech, high performance lighting devices has broadened the use of HID lamps in automobiles, video/data display and medical/technical applications. Short arc gap lamps (1mm) with a luminance exceeding that of the sun's surface (1600cd/mm2 -as observed from earth), and operating with extreme line broadening lead the video projection market. Low wattage HID lamps coupled with tailored optics can direct the light output more precisely leading to reduced light pollution and better system throughput. Tailoring of the driving electrical waveforms have enabled stable operation, controlled the effects of species segregation and improved lamp life and performance.

Adaptation of a visualized loop-mediated isothermal amplification technique for field detection of Plasmodium vivax infection.

PubMed

Tao, Zhi-Yong; Zhou, Hua-Yun; Xia, Hui; Xu, Sui; Zhu, Han-Wu; Culleton, Richard L; Han, Eun-Taek; Lu, Feng; Fang, Qiang; Gu, Ya-Ping; Liu, Yao-Bao; Zhu, Guo-Ding; Wang, Wei-Ming; Li, Ju-Lin; Cao, Jun; Gao, Qi

2011-06-21

Loop-mediated isothermal amplification (LAMP) is a high performance method for detecting DNA and holds promise for use in the molecular detection of infectious pathogens, including Plasmodium spp. However, in most malaria-endemic areas, which are often resource-limited, current LAMP methods are not feasible for diagnosis due to difficulties in accurately interpreting results with problems of sensitive visualization of amplified products, and the risk of contamination resulting from the high quantity of amplified DNA produced. In this study, we establish a novel visualized LAMP method in a closed-tube system, and validate it for the diagnosis of malaria under simulated field conditions. A visualized LAMP method was established by the addition of a microcrystalline wax-dye capsule containing the highly sensitive DNA fluorescence dye SYBR Green I to a normal LAMP reaction prior to the initiation of the reaction. A total of 89 blood samples were collected on filter paper and processed using a simple boiling method for DNA extraction, and then tested by the visualized LAMP method for Plasmodium vivax infection. The wax capsule remained intact during isothermal amplification, and released the DNA dye to the reaction mixture only when the temperature was raised to the melting point following amplification. Soon after cooling down, the solidified wax sealed the reaction mix at the bottom of the tube, thus minimizing the risk of aerosol contamination. Compared to microscopy, the sensitivity and specificity of LAMP were 98.3% (95% confidence interval (CI): 91.1-99.7%) and 100% (95% CI: 88.3-100%), and were in close agreement with a nested polymerase chain reaction method. This novel, cheap and quick visualized LAMP method is feasible for malaria diagnosis in resource-limited field settings.

Combined oxidative leaching and electrowinning process for mercury recovery from spent fluorescent lamps.

PubMed

Ozgur, Cihan; Coskun, Sezen; Akcil, Ata; Beyhan, Mehmet; Üncü, Ismail Serkan; Civelekoglu, Gokhan

2016-11-01

In this paper, oxidative leaching and electrowinnig processes were performed to recovery of mercury from spent tubular fluorescent lamps. Hypochlorite was found to be effectively used for the leaching of mercury to the solution. Mercury could be leached with an efficiency of 96% using 0.5M/0.2M NaOCl/NaCl reagents at 50°C and pH 7.5 for 2-h. Electrowinning process was conducted on the filtered leaching solutions and over the 81% of mercury was recovered at the graphite electrode using citric acid as a reducing agent. The optimal process conditions were observed as a 6A current intensity, 30g/L of reducing agent concentration, 120min. electrolysis time and pH of 7 at the room temperature. It was found that current intensity and citric acid amount had positive effect for mercury reduction. Recovery of mercury in its elemental form was confirmed by SEM/EDX. Oxidative leaching with NaOCl/NaCl reagent was followed by electrowinning process can be effectively used for the recovery of mercury from spent fluorescent lamps. Copyright © 2016 Elsevier Ltd. All rights reserved.

Rapid detection of Streptococcus pneumoniae by real-time fluorescence loop-mediated isothermal amplification

PubMed Central

Guo, Xu-Guang; Zhou, Shan

2014-01-01

Background and aim of study A significant human pathogenic bacterium, Streptococcus pneumoniae was recognized as a major cause of pneumonia, and is the subject of many humoral immunity studies. Diagnosis is generally made based on clinical suspicion along with a positive culture from a sample from virtually any place in the body. But the testing time is too long. This study is to establish a rapid diagnostic method to identification of Streptococcus pneumoniae. Methods Our laboratory has recently developed a new platform called real-amp, which combines loop-mediated isothermal amplification (LAMP) with a portable tube scanner real-time isothermal instrument for the rapid detection of Streptococcus pneumonia. Two pairs of amplification primers required for this method were derived from a conserved DNA sequence unique to the Streptococcus pneumoniae. The amplification was carried out at 63 degree Celsius using SYBR Green for 60 minutes with the tube scanner set to collect fluorescence signals. Clinical samples of Streptococcus pneumoniae and other bacteria were used to determine the sensitivity and specificity of the primers by comparing with traditional culture method. Results The new set of primers consistently detected in laboratory-maintained isolates of Streptococcus pneumoniae from our hospital. The new primers also proved to be more sensitive than the published species-specific primers specifically developed for the LAMP method in detecting Streptococcus pneumoniae. Conclusions This study demonstrates that the Streptococcus pneumoniae LAMP primers developed here have the ability to accurately detect Streptococcus pneumoniae infections by real-time fluorescence LAMP. PMID:25276360

Recovery of aluminium, nickel-copper alloys and salts from spent fluorescent lamps.

PubMed

Rabah, Mahmoud A

2004-01-01

This study explores a combined pyro-hydrometallurgical method to recover pure aluminium, nickel-copper alloy(s), and some valuable salts from spent fluorescent lamps (SFLs). It also examines the safe recycling of clean glass tubes for the fluorescent lamp industry. Spent lamps were decapped under water containing 35% acetone to achieve safe capture of mercury vapour. Cleaned glass tubes, if broken, were cut using a rotating diamond disc to a standard shorter length. Aluminium and copper-nickel alloys in the separated metallic parts were recovered using suitable flux to decrease metal losses going to slag. Operation variables affecting the quality of the products and the extent of recovery with the suggested method were investigated. Results revealed that total loss in the glass tube recycling operation was 2% of the SFLs. Pure aluminium meeting standard specification DIN 1712 was recovered by melting at 800 degrees C under sodium chloride/carbon flux for 20 min. Standard nickel-copper alloys with less than 0.1% tin were prepared by melting at 1250 degrees C using a sodium borate/carbon flux. De-tinning of the molten nickel-copper alloy was carried out using oxygen gas. Tin in the slag as oxide was recovered by reduction using carbon or hydrogen gas at 650-700 degrees C. Different valuable chloride salts were also obtained in good quality. Further research is recommended on the thermodynamics of nickel-copper recovery, yttrium and europium recovery, and process economics.

Investigations regarding the wet decontamination of fluorescent lamp waste using iodine in potassium iodide solutions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tunsu, Cristian, E-mail: tunsu@chalmers.se; Ekberg, Christian; Foreman, Mark

Highlights: • A wet-based decontamination process for fluorescent lamp waste is proposed. • Mercury can be leached using iodine in potassium iodide solution. • The efficiency of the process increases with an increase in leachant concentration. • Selective leaching of mercury from rare earth elements is achieved. • Mercury is furthered recovered using ion exchange, reduction or solvent extraction. - Abstract: With the rising popularity of fluorescent lighting, simple and efficient methods for the decontamination of discarded lamps are needed. Due to their mercury content end-of-life fluorescent lamps are classified as hazardous waste, requiring special treatment for disposal. A simplemore » wet-based decontamination process is required, especially for streams where thermal desorption, a commonly used but energy demanding method, cannot be applied. In this study the potential of a wet-based process using iodine in potassium iodide solution was studied for the recovery of mercury from fluorescent lamp waste. The influence of the leaching agent’s concentration and solid/liquid ratio on the decontamination efficiency was investigated. The leaching behaviour of mercury was studied over time, as well as its recovery from the obtained leachates by means of anion exchange, reduction, and solvent extraction. Dissolution of more than 90% of the contained mercury was achieved using 0.025/0.05 M I{sub 2}/KI solution at 21 °C for two hours. The efficiency of the process increased with an increase in leachant concentration. 97.3 ± 0.6% of the mercury contained was dissolved at 21 °C, in two hours, using a 0.25/0.5 M I{sub 2}/KI solution and a solid to liquid ratio of 10% w/v. Iodine and mercury can be efficiently removed from the leachates using Dowex 1X8 anion exchange resin or reducing agents such as sodium hydrosulphite, allowing the disposal of the obtained solution as non-hazardous industrial wastewater. The extractant CyMe{sub 4}BTBP showed good removal of mercury, with an extraction efficiency of 97.5 ± 0.7% being achieved in a single stage. Better removal of mercury was achieved in a single stage using the extractants Cyanex 302 and Cyanex 923 in kerosene, respectively.« less

The LAMP instrument at the Linac Coherent Light Source free-electron laser

NASA Astrophysics Data System (ADS)

Osipov, Timur; Bostedt, Christoph; Castagna, J.-C.; Ferguson, Ken R.; Bucher, Maximilian; Montero, Sebastian C.; Swiggers, Michele L.; Obaid, Razib; Rolles, Daniel; Rudenko, Artem; Bozek, John D.; Berrah, Nora

2018-03-01

The Laser Applications in Materials Processing (LAMP) instrument is a new end-station for soft X-ray imaging, high-field physics, and ultrafast X-ray science experiments that is available to users at the Linac Coherent Light Source (LCLS) free-electron laser. While the instrument resides in the Atomic, Molecular and Optical science hutch, its components can be used at any LCLS beamline. The end-station has a modular design that provides high flexibility in order to meet user-defined experimental requirements and specifications. The ultra-high-vacuum environment supports different sample delivery systems, including pulsed and continuous atomic, molecular, and cluster jets; liquid and aerosols jets; and effusive metal vapor beams. It also houses movable, large-format, high-speed pnCCD X-ray detectors for detecting scattered and fluorescent photons. Multiple charged-particle spectrometer options are compatible with the LAMP chamber, including a double-sided spectrometer for simultaneous and even coincident measurements of electrons, ions, and photons produced by the interaction of the high-intensity X-ray beam with the various samples. Here we describe the design and capabilities of the spectrometers along with some general aspects of the LAMP chamber and show some results from the initial instrument commissioning.

Laser Based Phosphor Converted Solid State White Light Emitters

NASA Astrophysics Data System (ADS)

Cantore, Michael

Artificial lighting and as a consequence the ability to be productive when the sun does not shine may be a profound achievement in society that is largely taken for granted. As concerns arise due to our dependence on energy sources with finite lifespan or environmentally negative effects, efforts to reduce energy consumption and create clean renewable alternatives has become highly valued. In the scope of artificial lighting, the use of incandescent lamps has shifted to more efficient light sources. Fluorescent lighting made the first big gains in efficiency over incandescent lamps with peak efficiency for mature designs reaching luminous efficacy of approximately 90 lm/W; more than three times as efficient as an incandescent lamp. Lamps based on light emitting diodes (LEDs) which can produce light at even greater efficiency, color quality and without the potential for hazardous chemical release from lamp failure. There is a significant challenge with LED based light sources. Their peak efficiency occurs at low current densities and then droops as the current density increases. Laser diodes (LDs) do not suffer from decreasing efficiency due to increased current. An alternative solid state light source using LDs has potential to make further gains in efficiency as well as allow novel illuminant designs which may be impractical or even impossible even with LED or other conventional sources. While similar to LEDS, the use of LDs does present new challenges largely due to the increased optical power density which must be accommodated in optics and phosphor materials. Single crystal YAG:Ce has been shown to be capable of enduring this more extreme operating environment while retaining the optical and fluorescing qualities desired for use as a wavelength converter in phosphor converted LD based white emitting systems. The incorporation of this single crystal phosphor in a system with a commercial laser diode with peak wall plug efficiency of 31% resulted in emission of white light with a luminous efficacy of 86.7 lm/W at a current of 1.4A. A total luminous flux of 1100 lm with luminous efficacy of 76 lm/W at 3.0 A current was achieved. Simulations have been conducted which show that as the InGaN LD technology matures towards the efficiencies of about 75%, which has been observed in the GaAs material system, luminous efficacy of similar blue LD with single crystal YAG:Ce systems will exceed 200 lm/W.

Temporal VUV Emission Characteristics Related to Generations and Losses of Metastable Atoms in Xenon Pulsed Barrier Discharge

NASA Astrophysics Data System (ADS)

Motomura, Hideki; Loo, Ka Hong; Ikeda, Yoshihisa; Jinno, Masafumi; Aono, Masaharu

Although xenon pulsed dielectric barrier discharge is one of the most promising substitutes for mercury low-pressure discharge for fluorescent lamps, the efficacy of xenon fluorescent lamp is not enough for practical use for general lighting. To improve the efficacy it is indispensable to clarify mechanisms of vacuum ultraviolet (VUV) emissions, which excite phosphor, from xenon discharge related to plasma characteristics. In this paper emission waveforms and temporal change of metastable atom density are measured and temporal VUV emission characteristics related to generations and losses of metastable atoms in xenon pulsed barrier discharge is investigated. It is shown that the lamp efficacy is improved by about 10% with shorter pulse in which the two VUV emission peaks in a pulse are overlapped. It is also shown that at the lower pressure of 1.3 kPa metastable atoms generated during on-period of the voltage pulse are not efficiently consumed for VUV emissions in the off-period of the voltage pulse because of lower rate of three-body collision and quenching. This fact is thought to be one of the reasons why the lamp efficacy is low at lower pressure.

Fluorescent lighting with aluminum nitride phosphors

DOEpatents

Cherepy, Nerine J.; Payne, Stephen A.; Seeley, Zachary M.; Srivastava, Alok M.

2016-05-10

A fluorescent lamp includes a glass envelope; at least two electrodes connected to the glass envelope; mercury vapor and an inert gas within the glass envelope; and a phosphor within the glass envelope, wherein the phosphor blend includes aluminum nitride. The phosphor may be a wurtzite (hexagonal) crystalline structure Al.sub.(1-x)M.sub.xN phosphor, where M may be drawn from beryllium, magnesium, calcium, strontium, barium, zinc, scandium, yttrium, lanthanum, cerium, praseodymium, europium, gadolinium, terbium, ytterbium, bismuth, manganese, silicon, germanium, tin, boron, or gallium is synthesized to include dopants to control its luminescence under ultraviolet excitation. The disclosed Al.sub.(1-x)M.sub.xN:Mn phosphor provides bright orange-red emission, comparable in efficiency and spectrum to that of the standard orange-red phosphor used in fluorescent lighting, Y.sub.2O.sub.3:Eu. Furthermore, it offers excellent lumen maintenance in a fluorescent lamp, and does not utilize "critical rare earths," minimizing sensitivity to fluctuating market prices for the rare earth elements.

Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes.

PubMed

Andrews, Norma W

2017-01-01

Elevation in the cytosolic Ca 2+ concentration triggers exocytosis of lysosomes in many cell types. This chapter describes a method to detect lysosomal exocytosis in mammalian cells, which takes advantage of the presence of an abundant glycoprotein, Lamp1, on the membrane of lysosomes. Lamp1 is a transmembrane protein with a large, heavily glycosylated region that faces the lumen of lysosomes. When lysosomes fuse with the plasma membrane, epitopes present on the luminal domain of Lamp1 are exposed on the cell surface. The Lamp1 luminal epitopes can then be detected on the surface of live, unfixed cells using highly specific monoclonal antibodies and fluorescence microscopy. The main advantage of this method is its sensitivity, and the fact that it provides spatial information on lysosomal exocytosis at the single cell level.

Epi-Fluorescence Microscopy

PubMed Central

Webb, Donna J.; Brown, Claire M.

2012-01-01

Epi-fluorescence microscopy is available in most life sciences research laboratories, and when optimized can be a central laboratory tool. In this chapter, the epi-fluorescence light path is introduced and the various components are discussed in detail. Recommendations are made for incident lamp light sources, excitation and emission filters, dichroic mirrors, objective lenses, and charge-coupled device (CCD) cameras in order to obtain the most sensitive epi-fluorescence microscope. The even illumination of metal-halide lamps combined with new “hard” coated filters and mirrors, a high resolution monochrome CCD camera, and a high NA objective lens are all recommended for high resolution and high sensitivity fluorescence imaging. Recommendations are also made for multicolor imaging with the use of monochrome cameras, motorized filter turrets, individual filter cubes, and corresponding dyes that are the best choice for sensitive, high resolution multicolor imaging. Images should be collected using Nyquist sampling and should be corrected for background intensity contributions and nonuniform illumination across the field of view. Photostable fluorescent probes and proteins that absorb a lot of light (i.e., high extinction co-efficients) and generate a lot of fluorescence signal (i.e., high quantum yields) are optimal. A neuronal immune-fluorescence labeling protocol is also presented. Finally, in order to maximize the utility of sensitive wide-field microscopes and generate the highest resolution images with high signal-to-noise, advice for combining wide-field epi-fluorescence imaging with restorative image deconvolution is presented. PMID:23026996

The benefits of future-proof tunable white lighting in the classroom

DOE PAGES

Clark, Terry

2016-11-21

The biggest challenge affecting lighting decisions today is future-proofing. Choices made today will likely be choices that impact the next 20 or perhaps even 40 years. For example, we can expect the best LED lighting available today to maintain 90% of initial light output for 100,000 hours or more. Compare this to a fluorescent lighting system that needs regular maintenance after only 30,000 hours due to lamp burn-outs. The simple act of choosing a LED system over a fluorescent one alone can begin to reduce life cycle costs by saving future maintenance costs. However, the choice of LED over fluorescentmore » is just the beginning. Here, let’s look at other future-proofing decisions that should be considered when choosing your next lighting system.« less

Dual-channel (green and red) fluorescence microendoscope with subcellular resolution

NASA Astrophysics Data System (ADS)

de Paula D'Almeida, Camila; Fortunato, Thereza Cury; Teixeira Rosa, Ramon Gabriel; Romano, Renan Arnon; Moriyama, Lilian Tan; Pratavieira, Sebastião.

2018-02-01

Usually, tissue images at cellular level need biopsies to be done. Considering this, diagnostic devices, such as microendoscopes, have been developed with the purpose of do not be invasive. This study goal is the development of a dual-channel microendoscope, using two fluorescent labels: proflavine and protoporphyrin IX (PpIX), both approved by Food and Drug Administration. This system, with the potential to perform a microscopic diagnosis and to monitor a photodynamic therapy (PDT) session, uses a halogen lamp and an image fiber bundle to perform subcellular image. Proflavine fluorescence indicates the nuclei of the cell, which is the reference for PpIX localization on image tissue. Preliminary results indicate the efficacy of this optical technique to detect abnormal tissues and to improve the PDT dosimetry. This was the first time, up to our knowledge, that PpIX fluorescence was microscopically observed in vivo, in real time, combined to other fluorescent marker (Proflavine), which allowed to simultaneously observe the spatial localization of the PpIX in the mucosal tissue. We believe this system is very promising tool to monitor PDT in mucosa as it happens. Further experiments have to be performed in order to validate the system for PDT monitoring.

Basic principles of fluorescence detection with use of 5-ALA

NASA Astrophysics Data System (ADS)

Baumgartner, Reinhold; Stepp, Herbert G.

2000-06-01

5-Aminolevulinic acid (5-ALA) has been proven to induce selective accumulation of flourescent Protoporphyrin IX (PPIX) in many types of malignant tissue. According to the target to treatment different routes of topical and systemical application of 5-ALA can be chosen. They include techniques like inhalation, installation and rinsing. For fluorescence detection a lamp based system have been developed in the laser-Forschungslabor in Munich together with Storz company. By skillful balancing of excitation filter centered around 400 nm and the observation filter with transmission above 450 nm images with high color contrast can be obtained. The universal application of the D-LIGHT could be demonstrated in different clinical disciplines like urology, neurosurgery, ENT clinic, gynecology and others.

UV reactor flow visualization and mixing quantification using three-dimensional laser-induced fluorescence.

PubMed

Gandhi, Varun; Roberts, Philip J W; Stoesser, Thorsten; Wright, Harold; Kim, Jae-Hong

2011-07-01

Three-dimensional laser-induced fluorescence (3DLIF) was applied to visualize and quantitatively analyze mixing in a lab-scale UV reactor consisting of one lamp sleeve placed perpendicular to flow. The recirculation zone and the von Karman vortex shedding that commonly occur in flows around bluff bodies were successfully visualized. Multiple flow paths were analyzed by injecting the dye at various heights with respect to the lamp sleeve. A major difference in these pathways was the amount of dye that traveled close to the sleeve, i.e., a zone of higher residence time and higher UV exposure. Paths away from the center height had higher velocities and hence minimal influence by the presence of sleeve. Approach length was also characterized in order to increase the probability of microbes entering the region around the UV lamp. The 3DLIF technique developed in this study is expected to provide new insight on UV dose delivery useful for the design and optimization of UV reactors. Copyright © 2011 Elsevier Ltd. All rights reserved.

Comparative Study of Light Sources for Household

NASA Astrophysics Data System (ADS)

Pawlak, Andrzej; Zalesińska, Małgorzata

2017-03-01

The article describes test results that provided the ground to define and evaluate basic photometric, colorimetric and electric parameters of selected, widely available light sources, which are equivalent to a traditional incandescent 60-Watt light bulb. Overall, one halogen light bulb, three compact fluorescent lamps and eleven LED light sources were tested. In general, it was concluded that in most cases (branded products, in particular) the measured and calculated parameters differ from the values declared by manufacturers only to a small degree. LED sources prove to be the most beneficial substitute for traditional light bulbs, considering both their operational parameters and their price, which is comparable with the price of compact fluorescent lamps or, in some instances, even lower.

Irradiance and spectral distribution control system for controlled environment chambers

NASA Technical Reports Server (NTRS)

Krones, M. J.; Sager, J. C.; Johnson, A. T.; Knott, W. M. (Principal Investigator)

1987-01-01

This paper describes a closed-loop control system for controlling the irradiance and spectral quality generated by fluorescent lamps in a controlled environment chamber. The 400 to 800 nm irradiance and the ratio of the red waveband (600 to 700 nm) to the far-red waveband (700 to 800 nm) were independently controlled and varied as functions of time. A suggested application is to investigate the possibility of synergistic effects of changing irradiance levels and changing spectral distributions on photoperiodism and photomorphogenesis.

Application of loop-mediated isothermal amplification (LAMP) assays for the detection of bovine herpesvirus 1.

PubMed

Socha, W; Rola, J; Urban-Chmiel, R; Żmudziński, J F

2017-09-26

Bovine herpesvirus-1 (BoHV-1), a causative agent of Infectious Bovine Rhinotracheitis (IBR), is responsible for high economic losses in cattle farming industry. The use of testing methods that allow early detection of BoHV-1-infected animals is a key element of each program of IBR eradication. The aim of the study was to design and evaluate two variants of LAMP isothermal tests with SYBR Green fluorescence probes, specific to the genes encoding gD and gE glycoproteins of BoHV-1. LAMP gE BoHV-1 assay was able to distinguish between gE- and gE+ strains of the virus. Both LAMP gD and gE assays were specific to BoHV-1 and did not react with other related to BoHV-1 alphaherpesviruses. Sensitivity of LAMP gD was 2x104 copies of the viral genome whereas for LAMP gE it was 2x105. Diagnostic sensitivity calculated for LAMP gD was 64.7% whereas for LAMP gE it was 80%. Diagnostic specificity for LAMP gD and LAMP gE was 78.9% and 89.3%, respectively. LAMP assay can be a rapid and simple method of diagnosis of acute BoHV-1 infections and discrimination of gE- strains. However, relatively low diagnostic sensitivity of the method can limit its use in routine diagnostics.

Reverse transcription loop-mediated isothermal amplification (RT-LAMP), a light for mammalian transcript analysis in low-input laboratories.

PubMed

Pandey, Mamta; Singh, Dheer; Onteru, Suneel K

2018-06-01

Transcript analysis is usually performed by costly, time-consuming, and expertise intensive methods, like real time-PCR, microarray, etc. However, they are not much feasible in low-input laboratories. Therefore, we implemented the reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a means of mammalian transcript analysis. Particularly, RT-LAMP was developed for buffalo aromatase cytochrome P450 (CYP19) transcript, to study its expression in 3D-cultured buffalo granulosa cells, which were exposed to lipopolysaccharide (LPS). The CYP19-RT-LAMP assay rapidly identified the LPS-induced downregulation of the CYP19 gene within 30 min at 63°C in a water bath. The assay was visualized via unaided eye by observing the change in turbidity and fluorescence, which were decreased by increasing the LPS exposure time to granulosa cells. Overall, the developed CYP19-RT-LAMP assay provided a hope on the application of RT-LAMP for mammalian transcript analysis in low-input laboratories. © 2017 Wiley Periodicals, Inc.

Selective extraction and recovery of rare earth metals from phosphor powders in waste fluorescent lamps using an ionic liquid system.

PubMed

Yang, Fan; Kubota, Fukiko; Baba, Yuzo; Kamiya, Noriho; Goto, Masahiro

2013-06-15

The recycling of rare earth metals from phosphor powders in waste fluorescent lamps by solvent extraction using ionic liquids was studied. Acid leaching of rare earth metals from the waste phosphor powder was examined first. Yttrium (Y) and europium (Eu) dissolved readily in the acid solution; however, the leaching of other rare earth metals required substantial energy input. Ionization of target rare earth metals from the waste phosphor powders into the leach solution was critical for their successful recovery. As a high temperature was required for the complete leaching of all rare earth metals, ionic liquids, for which vapor pressure is negligible, were used as an alternative extracting phase to the conventional organic diluent. An extractant, N, N-dioctyldiglycol amic acid (DODGAA), which was recently developed, showed a high affinity for rare earth metal ions in liquid-liquid extraction although a conventional commercial phosphonic extractant did not. An effective recovery of the rare earth metals, Y, Eu, La and Ce, from the metal impurities, Fe, Al and Zn, was achieved from the acidic leach solution of phosphor powders using an ionic liquid containing DODGAA as novel extractant system. Copyright © 2013 Elsevier B.V. All rights reserved.

A Simple Strategy in Avulsion Flap Injury: Prediction of Flap Viability Using Wood's Lamp Illumination and Resurfacing with a Full-thickness Skin Graft.

PubMed

Lim, Hyoseob; Han, Dae Hee; Lee, Il Jae; Park, Myong Chul

2014-03-01

Extensive degloving injuries of the extremities usually result in necrosis of the flap, necessitating comprehensive skin grafting. Provided there is a sufficient tool to evaluate flap viability, full-thickness skin can be used from a nonviable avulsed flap. We used a Wood's lamp to determine the viability of avulsed flaps in the operation field after intravenous injection of fluorescein dye. We experienced 13 cases during 16 months. Fifteen minutes after the intravenous injection of fluorescein dye, the avulsed skin flaps were examined and non-fluorescent areas were marked under Wood's lamp illumination. The marked area was defatted for full-thickness skin grafting. The fluorescent areas were sutured directly without tension. The non-fluorescent areas were covered by defatted skin. Several days later, there was soft tissue necrosis within the flap area. We measured necrotic area and revised the flap. Among all the cases, necrotic area was 21.3% of the total avulsed area. However, if we exclude three cases, one of a carelessly managed patient and two cases of the flaps were inappropriately applied, good results were obtained, with a necrotic area of only 8.4%. Eight patients needed split-thickness skin grafts, and heel pad reconstruction was performed with free flap. A full-thickness skin graft from an avulsed flap is a good method for addressing aesthetic concerns without producing donor site morbidity. Fluorescein dye is a useful, simple, and cost-effective tool for evaluating flap viability. Avulsed flap injuries can be managed well with Wood's lamp illumination and a full-thickness skin graft.

Numerical study on xenon positive column discharges of mercury-free lamp

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ouyang, Jiting; He, Feng; Miao, Jinsong

2007-02-15

In this paper, the numerical study has been performed on the xenon positive column discharges of mercury-free fluorescent lamp. The plasma discharge characteristics are analyzed by numerical simulation based on two-dimensional fluid model. The effects of cell geometry, such as the dielectric layer, the electrode width, the electrode gap, and the cell height, and the filling gas including the pressure and the xenon percentage are investigated in terms of discharge current and discharge efficiency. The results show that a long transient positive column will form in the xenon lamp when applying ac sinusoidal power and the lamp can operate inmore » a large range of voltage and frequency. The front dielectric layer of the cell plays an important role in the xenon lamp while the back layer has little effect. The ratio of electrode gap to cell height should be large to achieve a long positive column xenon lamp and higher efficiency. Increase of pressure or xenon concentration results in an increase of discharge efficiency and voltage. The discussions will be helpful for the design of commercial xenon lamp cells.« less

78 FR 20946 - Certain Dimmable Compact Fluorescent Lamps and Products Containing Same; Notice of Request for...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-08

... INTERNATIONAL TRADE COMMISSION [Investigation No. 337-TA-830] Certain Dimmable Compact Fluorescent.... International Trade Commission. ACTION: Notice. SUMMARY: Notice is hereby given that the presiding... General Counsel, U.S. International Trade Commission, 500 E Street SW., Washington, DC 20436, telephone...

10 CFR 430.25 - Laboratory Accreditation Program.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Procedures § 430.25 Laboratory Accreditation Program. Testing for fluorescent lamp ballasts performed in accordance with appendix Q1 to this subpart shall comply with this section § 430.25. The testing for general... performed in accordance with appendix R to this subpart. The testing for medium base compact fluorescent...

LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes

PubMed Central

Peschel, Andrea; Langer, Brigitte; Gröger, Marion; Rees, Andrew; Kain, Renate

2016-01-01

ABSTRACT Autophagy is an evolutionarily conserved process used for removing surplus and damaged proteins and organelles from the cytoplasm. The unwanted material is incorporated into autophagosomes that eventually fuse with lysosomes, leading to the degradation of their cargo. The fusion event is mediated by the interaction between the Qa-SNARE syntaxin-17 (STX17) on autophagosomes and the R-SNARE VAMP8 on lysosomes. Cells deficient in lysosome membrane-associated protein-2 (LAMP-2) have increased numbers of autophagosomes but the underlying mechanism is poorly understood. By transfecting LAMP-2-deficient and LAMP-1/2­-double-deficient mouse embryonic fibroblasts (MEFs) with a tandem fluorescent-tagged LC3 we observed a failure of fusion between the autophagosomes and the lysosomes that could be rescued by complementation with LAMP-2A. Although we observed no change in expression and localization of VAMP8, its interacting partner STX17 was absent from autophagosomes of LAMP-2-deficient cells. Thus, LAMP-2 is essential for STX17 expression by the autophagosomes and this absence is sufficient to explain their failure to fuse with lysosomes. The results have clear implications for situations associated with a reduction of LAMP-2 expression. PMID:27628032

Occurrence of refractive errors among students who before the age of two grew up under the influence of light emitted by incandescent or fluorescent lamps.

PubMed

Czepita, Damian; Gosławski, Wojciech; Mojsa, Artur

2005-01-01

The aim of the study was to determine whether the development of refractive errors could be associated with exposure to light emitted by incandescent or fluorescent lamps. 3636 students were examined (1638 boys and 1998 girls, aged 6-18 years, mean age 12.1, SD 3.4). The examination included skiascopy with cycloplegia. Myopia was defined as refractive error < or = -0.5 D, hyperopia as refractive error > or = +1.5 D, astigmatism as refractive error > 0.5 DC. Anisometropia was diagnosed when the difference in the refraction of both eyes was > 1.0 D. The parents of all the students examined completed a questionnaire on the child's light exposure before the age oftwo. Data were analyzed statistically with the chi2 test. P values of less than 0.05 were considered statistically significant. It was observed that sleeping until the age of two in a room with a light turned on is associated with an increase in the occurrence of anisometropia (p < 0.02) as well as with a reduction in the prevalence of emmetropia (p < 0.05). It was also found that light emitted by fluorescent lamps leads to more frequent occurrence of astigmatism (p < 0.01).

Cutaneous porphyrins exhibit anti-stokes fluorescence that is detectable in sebum (Conference Presentation)

NASA Astrophysics Data System (ADS)

Tian, Giselle; Zeng, Haishan; Zhao, Jianhua; Wu, Zhenguo; Al Jasser, Mohammed; Lui, Harvey; Mclean, David I.

2016-02-01

Porphyrins produced by Propionibacterium acnes represent the principal fluorophore associated with acne, and appear as orange-red luminescence under the Wood's lamp. Assessment of acne based on Wood's lamp (UV) or visible light illumination is limited by photon penetration depth and has limited sensitivity for earlier stage lesions. Inducing fluorescence with near infrared (NIR) excitation may provide an alternative way to assess porphyrin-related skin disorders. We discovered that under 785 nm CW laser excitation PpIX powder exhibits fluorescence emission in the shorter wavelength range of 600-715 nm with an intensity that is linearly dependent on the excitation power. We attribute this shorter wavelength emission to anti-Stokes fluorescence. Similar anti-Stokes fluorescence was also detected focally in all skin-derived samples containing porphyrins. Regular (Stokes) fluorescence was present under UV and visible light excitation on ex vivo nasal skin and sebum from uninflamed acne, but not on nose surface smears or sebum from inflamed acne. Co-registered CW laser-excited anti-Stokes fluorescence and fs laser-excited multi-photon fluorescence images of PpIX powder showed similar features. In the skin samples because of the anti-Stokes effect, the NIR-induced fluorescence was presumably specific for porphyrins since there appeared to be no anti-Stokes emission signals from other typical skin fluorophores such as lipids, keratins and collagen. Anti-Stokes fluorescence under NIR CW excitation is more sensitive and specific for porphyrin detection than UV- or visible light-excited regular fluorescence and fs laser-excited multi-photon fluorescence. This approach also has higher image contrast compared to NIR fs laser-based multi-photon fluorescence imaging. The anti-Stokes fluorescence of porphyrins within sebum could potentially be applied to detecting and targeting acne lesions for treatment via fluorescence image guidance.

Which lamp will be optimum to eye? Incandescent, fluorescent or LED etc

PubMed Central

Chen, Liang; Zhang, Xiao-Wei

2014-01-01

Low frequency flicker, high frequency flicker, strong light, strong blue light, infrared, ultraviolet, electromagnetic radiation, ripple flicker and dimming flicker produced by different lamps have negative impact on vision, eyes and health. Negative impact on eyes resulting in myopia or cataract etc: the solution is to remove all the negative factors by applying upright lighting technology and that is optimum to vision, eyes and health. PMID:24634884

Which lamp will be optimum to eye? Incandescent, fluorescent or LED etc.

PubMed

Chen, Liang; Zhang, Xiao-Wei

2014-01-01

Low frequency flicker, high frequency flicker, strong light, strong blue light, infrared, ultraviolet, electromagnetic radiation, ripple flicker and dimming flicker produced by different lamps have negative impact on vision, eyes and health. Negative impact on eyes resulting in myopia or cataract etc: the solution is to remove all the negative factors by applying upright lighting technology and that is optimum to vision, eyes and health.

Light-emitting diodes as an illumination source for plants: a review of research at Kennedy Space Center

NASA Technical Reports Server (NTRS)

Kim, Hyeon-Hye; Wheeler, Raymond M.; Sager, John C.; Yorio, Neil C.; Goins, Gregory D.

2005-01-01

The provision of sufficient light is a fundamental requirement to support long-term plant growth in space. Several types of electric lamps have been tested to provide radiant energy for plants in this regard, including fluorescent, high-pressure sodium, and metal halide lamps. These lamps vary in terms of spectral quality, which can result in differences in plant growth and morphology. Current lighting research for space-based plant culture is focused on innovative lighting technologies that demonstrate high electrical efficiency and reduced mass and volume. Among the lighting technologies considered for space are light-emitting diodes (LEDs). The combination of red and blue LEDs has proven to be an effective lighting source for several crops, yet the appearance of plants under red and blue lighting is purplish gray, making visual assessment of plant health difficult. Additional green light would make the plant leaves appear green and normal, similar to a natural setting under white light, and may also offer psychological benefits for the crew. The addition of 24% green light (500-600 nm) to red and blue LEDs enhanced the growth of lettuce plants compared with plants grown under cool white fluorescent lamps. Coincidentally, these plants grown under additional green light would have the additional aesthetic appeal of a green appearance.

Efficacy of photodynamic therapy against larvae of Aedes aegypti: confocal microscopy and fluorescence-lifetime imaging

NASA Astrophysics Data System (ADS)

de Souza, L. M.; Pratavieira, S.; Inada, N. M.; Kurachi, C.; Corbi, J.; Guimarães, F. E. G.; Bagnato, V. S.

2014-03-01

Recently a few demonstration on the use of Photodynamic Reaction as possibility to eliminate larvae that transmit diseases for men has been successfully demonstrated. This promising tool cannot be vastly used due to many problems, including the lake of investigation concerning the mechanisms of larvae killing as well as security concerning the use of photosensitizers in open environment. In this study, we investigate some of the mechanisms in which porphyrin (Photogem) is incorporated on the Aedes aegypti larvae previously to illumination and killing. Larvae at second instar were exposed to the photosensitizer and after 30 minutes imaged by a confocal fluorescence microscope. It was observed the presence of photosensitizer in the gut and at the digestive tract of the larva. Fluorescence-Lifetime Imaging showed greater photosensitizer concentration in the intestinal wall of the samples, which produces a strong decrease of the Photogem fluorescence lifetime. For Photodynamic Therapy exposition to different light doses and concentrations of porphyrin were employed. Three different light sources (LED, Fluorescent lamp, Sun light) also were tested. Sun light and fluorescent lamp shows close to 100% of mortality after 24 hrs. of illumination. These results indicate the potential use of photodynamic effect against the LARVAE of Aedes aegypti.

Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP).

PubMed

Thanarajoo, Sathis Sri; Kong, Lih Ling; Kadir, Jugah; Lau, Wei Hongi; Vadamalai, Ganesan

2014-06-01

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60 min at 60 °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix after the addition of fluorescent reagent, and a laddering pattern band on 2% agarose gel electrophoresis. Conventional RT-PCR with LAMP primers produced amplicons with a sequence identical to the 297-nt CCCVd oil palm variant with the primers being specific for CCCVd and not for other viroids such as PSTVd and CEVd. RT-LAMP was found to be rapid and specific for detecting oil palm CCCVd. Copyright © 2014 Elsevier B.V. All rights reserved.

Development of a highly sensitive loop-mediated isothermal amplification (LAMP) method for the detection of Loa loa.

PubMed

Fernández-Soto, Pedro; Mvoulouga, Prosper Obolo; Akue, Jean Paul; Abán, Julio López; Santiago, Belén Vicente; Sánchez, Miguel Cordero; Muro, Antonio

2014-01-01

The filarial parasite Loa loa, the causative agent of loiasis, is endemic in Central and Western Africa infecting 3-13 million people. L. loa has been associated with fatal encephalopathic reactions in high Loa-infected individuals receiving ivermectin during mass drug administration programs for the control of onchocerciasis and lymphatic filariasis. In endemic areas, the only diagnostic method routinely used is the microscopic examination of mid-day blood samples by thick blood film. Improved methods for detection of L. loa are needed in endemic regions with limited resources, where delayed diagnosis results in high mortality. We have investigated the use of a loop-mediated isothermal amplification (LAMP) assay to facilitate rapid, inexpensive, molecular diagnosis of loiasis. Primers for LAMP were designed from a species-specific repetitive DNA sequence from L. loa retrieved from GenBank. Genomic DNA of a L. loa adult worm was used to optimize the LAMP conditions using a thermocycler or a conventional heating block. Amplification of DNA in the LAMP mixture was visually inspected for turbidity as well as addition of fluorescent dye. LAMP specificity was evaluated using DNA from other parasites; sensitivity was evaluated using DNA from L. loa 10-fold serially diluted. Simulated human blood samples spiked with DNA from L. loa were also tested for sensitivity. Upon addition of fluorescent dye, all positive reactions turned green while the negative controls remained orange under ambient light. After electrophoresis on agarose gels, a ladder of multiple bands of different sizes could be observed in positive samples. The detection limit of the assay was found to be as little as 0.5 ag of L. loa genomic DNA when using a heating block. We have designed, for the first time, a highly sensitive LAMP assay for the detection of L. loa which is potentially adaptable for field diagnosis and disease surveillance in loiasis-endemic areas.

Development of a Highly Sensitive Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Loa loa

PubMed Central

Fernández-Soto, Pedro; Mvoulouga, Prosper Obolo; Akue, Jean Paul; Abán, Julio López; Santiago, Belén Vicente; Sánchez, Miguel Cordero; Muro, Antonio

2014-01-01

The filarial parasite Loa loa, the causative agent of loiasis, is endemic in Central and Western Africa infecting 3–13 million people. L. loa has been associated with fatal encephalopathic reactions in high Loa-infected individuals receiving ivermectin during mass drug administration programs for the control of onchocerciasis and lymphatic filariasis. In endemic areas, the only diagnostic method routinely used is the microscopic examination of mid-day blood samples by thick blood film. Improved methods for detection of L. loa are needed in endemic regions with limited resources, where delayed diagnosis results in high mortality. We have investigated the use of a loop-mediated isothermal amplification (LAMP) assay to facilitate rapid, inexpensive, molecular diagnosis of loiasis. Primers for LAMP were designed from a species-specific repetitive DNA sequence from L. loa retrieved from GenBank. Genomic DNA of a L. loa adult worm was used to optimize the LAMP conditions using a thermocycler or a conventional heating block. Amplification of DNA in the LAMP mixture was visually inspected for turbidity as well as addition of fluorescent dye. LAMP specificity was evaluated using DNA from other parasites; sensitivity was evaluated using DNA from L. loa 10-fold serially diluted. Simulated human blood samples spiked with DNA from L. loa were also tested for sensitivity. Upon addition of fluorescent dye, all positive reactions turned green while the negative controls remained orange under ambient light. After electrophoresis on agarose gels, a ladder of multiple bands of different sizes could be observed in positive samples. The detection limit of the assay was found to be as little as 0.5 ag of L. loa genomic DNA when using a heating block. We have designed, for the first time, a highly sensitive LAMP assay for the detection of L. loa which is potentially adaptable for field diagnosis and disease surveillance in loiasis-endemic areas. PMID:24722638

The LAMP instrument at the Linac Coherent Light Source free-electron laser

DOE PAGES

Osipov, Timur; Bostedt, Christoph; Castagna, J. -C.; ...

2018-03-23

The Laser Applications in Materials Processing (LAMP) instrument is a new end-station for soft X-ray imaging, high-field physics, and ultrafast X-ray science experiments that is available to users at the Linac Coherent Light Source (LCLS) free-electron laser. While the instrument resides in the Atomic, Molecular and Optical science hutch, its components can be used at any LCLS beamline. The end-station has a modular design that provides high flexibility in order to meet user-defined experimental requirements and specifications. The ultra-high-vacuum environment supports different sample delivery systems, including pulsed and continuous atomic, molecular, and cluster jets; liquid and aerosols jets; and effusivemore » metal vapor beams. It also houses movable, large-format, high-speed pnCCD X-ray detectors for detecting scattered and fluorescent photons. Multiple charged-particle spectrometer options are compatible with the LAMP chamber, including a double-sided spectrometer for simultaneous and even coincident measurements of electrons, ions, and photons produced by the interaction of the high-intensity X-ray beam with the various samples. Here in this paper we describe the design and capabilities of the spectrometers along with some general aspects of the LAMP chamber and show some results from the initial instrument commissioning.« less

The LAMP instrument at the Linac Coherent Light Source free-electron laser

DOE Office of Scientific and Technical Information (OSTI.GOV)

Osipov, Timur; Bostedt, Christoph; Castagna, J. -C.

The Laser Applications in Materials Processing (LAMP) instrument is a new end-station for soft X-ray imaging, high-field physics, and ultrafast X-ray science experiments that is available to users at the Linac Coherent Light Source (LCLS) free-electron laser. While the instrument resides in the Atomic, Molecular and Optical science hutch, its components can be used at any LCLS beamline. The end-station has a modular design that provides high flexibility in order to meet user-defined experimental requirements and specifications. The ultra-high-vacuum environment supports different sample delivery systems, including pulsed and continuous atomic, molecular, and cluster jets; liquid and aerosols jets; and effusivemore » metal vapor beams. It also houses movable, large-format, high-speed pnCCD X-ray detectors for detecting scattered and fluorescent photons. Multiple charged-particle spectrometer options are compatible with the LAMP chamber, including a double-sided spectrometer for simultaneous and even coincident measurements of electrons, ions, and photons produced by the interaction of the high-intensity X-ray beam with the various samples. Here in this paper we describe the design and capabilities of the spectrometers along with some general aspects of the LAMP chamber and show some results from the initial instrument commissioning.« less

Light Spectral Quality Effects on the Growth of Potato (Solanum Tuberosum L.) Nodal Cuttings in Vitro

NASA Technical Reports Server (NTRS)

Wilson, Deborah A.; Weigel, Russell C.; Wheeler, Raymond M.; Sager, John C.

1993-01-01

The effects of light spectral quality on the growth of in vitro nodal cuttings of potato (Solanum tuberosum L.) cultivars Norland, Superior, Kennebec, and Denali were examined. The different light spectra were provided by Vita-Lite fluorescent (VF) (a white light control), blue fluorescent (BF), red fluorescent (RF), low-pressure sodium (LPS), and a combination of low-pressure sodium plus cool-white fluorescent lamps (LPS/CWF). For cultivars, stem lengths after 4 wks were longest under LPS, follow by RF, LPS/CWF, VF, and BF (in descending order). Microscopic studies revealed that cells were shortest when cultured in BF or VF environments, and were longest in RF or LPS lamp environments. The highest number axillary branches occurred on plantlets grown with LPS or LPS/CWF, whereas the lowest number occurred with BF. No leaf or stem edema (callus or gall-like growths) occurred iwth LPS or LPS/cwf lighting, and no edema occurred on cv. Norland plantlets, regardless of lighting. Results suggest that shoot morphologic development of in vitro grown potato plants can be controlled by controlling irradiant spectral quality.

NASA-sponsored containerless processing experiments

NASA Technical Reports Server (NTRS)

Hofmeister, William H.

1990-01-01

An outline is presented of containerless processing and facilities at Intersonics which is sponsored by NASA. There are electromagnetic, acoustic, and aerodynamic levitation facilities. There are also laser beam and arc lamp heating systems along with state of the art noncontact temperature and optical property measurement facilities. Nonintrusive diagnostic techniques with Laser Induced Fluorescence and mass spectrometer are also available. Controlled atmosphere processing, gas quenching, and proven microgravity processing technology is part of the Intersonics capabilities.

A Paper-Based Device for Performing Loop-Mediated Isothermal Amplification with Real-Time Simultaneous Detection of Multiple DNA Targets.

PubMed

Seok, Youngung; Joung, Hyou-Arm; Byun, Ju-Young; Jeon, Hyo-Sung; Shin, Su Jeong; Kim, Sanghyo; Shin, Young-Beom; Han, Hyung Soo; Kim, Min-Gon

2017-01-01

Paper-based diagnostic devices have many advantages as a one of the multiple diagnostic test platforms for point-of-care (POC) testing because they have simplicity, portability, and cost-effectiveness. However, despite high sensitivity and specificity of nucleic acid testing (NAT), the development of NAT based on a paper platform has not progressed as much as the others because various specific conditions for nucleic acid amplification reactions such as pH, buffer components, and temperature, inhibitions from technical differences of paper-based device. Here, we propose a paper-based device for performing loop-mediated isothermal amplification (LAMP) with real-time simultaneous detection of multiple DNA targets. We determined the optimal chemical components to enable dry conditions for the LAMP reaction without lyophilization or other techniques. We also devised the simple paper device structure by sequentially stacking functional layers, and employed a newly discovered property of hydroxynaphthol blue fluorescence to analyze real-time LAMP signals in the paper device. This proposed platform allowed analysis of three different meningitis DNA samples in a single device with single-step operation. This LAMP-based multiple diagnostic device has potential for real-time analysis with quantitative detection of 10 2 -10 5 copies of genomic DNA. Furthermore, we propose the transformation of DNA amplification devices to a simple and affordable paper system approach with great potential for realizing a paper-based NAT system for POC testing.

A Paper-Based Device for Performing Loop-Mediated Isothermal Amplification with Real-Time Simultaneous Detection of Multiple DNA Targets

PubMed Central

Seok, Youngung; Joung, Hyou-Arm; Byun, Ju-Young; Jeon, Hyo-Sung; Shin, Su Jeong; Kim, Sanghyo; Shin, Young-Beom; Han, Hyung Soo; Kim, Min-Gon

2017-01-01

Paper-based diagnostic devices have many advantages as a one of the multiple diagnostic test platforms for point-of-care (POC) testing because they have simplicity, portability, and cost-effectiveness. However, despite high sensitivity and specificity of nucleic acid testing (NAT), the development of NAT based on a paper platform has not progressed as much as the others because various specific conditions for nucleic acid amplification reactions such as pH, buffer components, and temperature, inhibitions from technical differences of paper-based device. Here, we propose a paper-based device for performing loop-mediated isothermal amplification (LAMP) with real-time simultaneous detection of multiple DNA targets. We determined the optimal chemical components to enable dry conditions for the LAMP reaction without lyophilization or other techniques. We also devised the simple paper device structure by sequentially stacking functional layers, and employed a newly discovered property of hydroxynaphthol blue fluorescence to analyze real-time LAMP signals in the paper device. This proposed platform allowed analysis of three different meningitis DNA samples in a single device with single-step operation. This LAMP-based multiple diagnostic device has potential for real-time analysis with quantitative detection of 102-105 copies of genomic DNA. Furthermore, we propose the transformation of DNA amplification devices to a simple and affordable paper system approach with great potential for realizing a paper-based NAT system for POC testing. PMID:28740546

Fluorescence spectroscopy for endogenous porphyrins in human facial skin

NASA Astrophysics Data System (ADS)

Seo, I.; Tseng, S. H.; Cula, G. O.; Bargo, P. R.; Kollias, N.

2009-02-01

The activity of certain bacteria in skin is known to correlate to the presence of porphyrins. In particular the presence of coproporphyrin produced by P.acnes inside plugged pores has been correlated to acne vulgaris. Another porphyrin encountered in skin is protoporphyrin IX, which is produced by the body in the pathway for production of heme. In the present work, a fluorescence spectroscopy system was developed to measure the characteristic spectrum and quantify the two types of porphyrins commonly present in human facial skin. The system is comprised of a Xe lamp both for fluorescence excitation and broadband light source for diffuse reflectance measurements. A computer-controlled filter wheel enables acquisition of sequential spectra, first excited by blue light at 405 nm then followed by the broadband light source, at the same location. The diffuse reflectance spectrum was used to correct the fluorescence spectrum due to the presence of skin chromophores, such as blood and melanin. The resulting fluorescence spectra were employed for the quantification of porphyrin concentration in a population of healthy subjects. The results show great variability on the concentration of these porphyrins and further studies are being conducted to correlate them with skin conditions such as inflammation and acne vulgaris.

Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.

PubMed

Wong, Y-P; Othman, S; Lau, Y-L; Radu, S; Chee, H-Y

2018-03-01

Loop-mediated isothermal amplification (LAMP) amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions by using a DNA polymerase with high displacement strand activity and a set of specifically designed primers to amplify targeted DNA strands. Following its first discovery by Notomi et al. ( Nucleic Acids Res 28: E63), LAMP was further developed over the years which involved the combination of this technique with other molecular approaches, such as reverse transcription and multiplex amplification for the detection of infectious diseases caused by micro-organisms in humans, livestock and plants. In this review, available types of LAMP techniques will be discussed together with their applications in detection of various micro-organisms. Up to date, there are varieties of LAMP detection methods available including colorimetric and fluorescent detection, real-time monitoring using turbidity metre and detection using lateral flow device which will also be highlighted in this review. Apart from that, commercialization of LAMP technique had also been reported such as lyophilized form of LAMP reagents kit and LAMP primer sets for detection of pathogenic micro-organisms. On top of that, advantages and limitations of this molecular detection method are also described together with its future potential as a diagnostic method for infectious disease. © 2017 The Society for Applied Microbiology.

LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes.

PubMed

Hubert, Virginie; Peschel, Andrea; Langer, Brigitte; Gröger, Marion; Rees, Andrew; Kain, Renate

2016-10-15

Autophagy is an evolutionarily conserved process used for removing surplus and damaged proteins and organelles from the cytoplasm. The unwanted material is incorporated into autophagosomes that eventually fuse with lysosomes, leading to the degradation of their cargo. The fusion event is mediated by the interaction between the Qa-SNARE syntaxin-17 (STX17) on autophagosomes and the R-SNARE VAMP8 on lysosomes. Cells deficient in lysosome membrane-associated protein-2 (LAMP-2) have increased numbers of autophagosomes but the underlying mechanism is poorly understood. By transfecting LAMP-2-deficient and LAMP-1/2--double-deficient mouse embryonic fibroblasts (MEFs) with a tandem fluorescent-tagged LC3 we observed a failure of fusion between the autophagosomes and the lysosomes that could be rescued by complementation with LAMP-2A. Although we observed no change in expression and localization of VAMP8, its interacting partner STX17 was absent from autophagosomes of LAMP-2-deficient cells. Thus, LAMP-2 is essential for STX17 expression by the autophagosomes and this absence is sufficient to explain their failure to fuse with lysosomes. The results have clear implications for situations associated with a reduction of LAMP-2 expression. © 2016. Published by The Company of Biologists Ltd.

Comparison of methodologies for conidia production by Alternaria alternata from citrus

PubMed Central

Carvalho, Daniel D. C.; Alves, Eduardo; Batista, Tereza R. S.; Camargos, Renato B.; Lopes, Eloísa A. G. L.

2008-01-01

Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isolate). The methodologies used were conidia production with 12 and 24 hours under white fluorescent light, evaluation with 24 and 48 hours after applying fungal mycelium stress technique, cold stress followed by injury of mycelium and evaluation with 24 hours, using healthy vegetable tissue and the use of black fluorescent near ultraviolet (NUV) lamp. Satisfactory result was obtained with A. alternata isolate from Murcott tangor, with the production of 2.8 × 105 conidia mL-1, when fungal mycelium was stressed (Petri dish with 66.66% of fungi growth) and subsequently 24 h of growth. The use of white light (24 h) and black fluorescent NUV lamp also induced expressive conidia production by one isolate of Ponkan tangerine, which produced 17.2 × 105 and 10.1 × 105conidia mL-1 and another of Murcott tangor, which produced 13.9 × 105 and 10.1 × 105 conidia mL-1, respectively. The remaining methodologies analyzed in this study were not able to induce conidia production in satisfactory quantity. The use of both mycelium stress technique and white light (24 h) and black fluorescent NUV lamp allowed the production of enough quantities of conidia to be used in vitro (detection of fungitoxic substances) and in vivo (pathogenicity test) assays, respectively. PMID:24031309

Detection of Shigella in Milk and Clinical Samples by Magnetic Immunocaptured-Loop-Mediated Isothermal Amplification Assay

PubMed Central

Zhang, Liding; Wei, Qiujiang; Han, Qinqin; Chen, Qiang; Tai, Wenlin; Zhang, Jinyang; Song, Yuzhu; Xia, Xueshan

2018-01-01

Shigella is an important human food-borne zoonosis bacterial pathogen, and can cause clinically severe diarrhea. There is an urgent need to develop a specific, sensitive, and rapid methodology for detection of this pathogen. In this study, loop-mediated isothermal amplification (LAMP) combined with magnetic immunocapture assay (IC-LAMP) was first developed for the detection of Shigella in pure culture, artificial milk, and clinical stool samples. This method exhibited a detection limit of 8.7 CFU/mL. Compared with polymerase chain reaction, IC-LAMP is sensitive, specific, and reliable for monitoring Shigella. Additionally, IC-LAMP is more convenient, efficient, and rapid than ordinary LAMP, as it is more efficiently enriches pathogen cells without extraction of genomic DNA. Under isothermal conditions, the amplification curves and the green fluorescence were detected within 30 min in the presence of genomic DNA template. The overall analysis time was approximately 1 h, including the enrichment and lysis of the bacterial cells, a significantly short detection time. Therefore, the IC-LAMP methodology described here is potentially useful for the efficient detection of Shigella in various samples. PMID:29467730

Characterization and calibration of a combined laser Raman, fluorescence and coherent Raman spectrometer

NASA Astrophysics Data System (ADS)

Lawhead, Carlos; Cooper, Nathan; Anderson, Josiah; Shiver, Tegan; Ujj, Laszlo

2014-03-01

Electronic and vibrational spectroscopy is extremely important tools used in material characterization; therefore a table-top laser spectrometer system was built in the spectroscopy lab at the UWF physics department. The system is based upon an injection seeded nanosecond Nd:YAG Laser. The second and the third harmonics of the fundamental 1064 nm radiation are used to generate Raman and fluorescence spectra measured with MS260i imaging spectrograph occupied with a CCD detector and cooled to -85 °C, in order to minimize the dark background noise. The wavelength calibration was performed with the emission spectra of standard gas-discharge lamps. Spectral sensitivity calibration is needed before any spectra are recorded, because of the table-top nature of the instrument. A variety of intensity standards were investigated to find standards suitable for our table top setup that do not change the geometry of the system. High quality measurement of Raman standards where analyzed to test spectral corrections. Background fluorescence removal methods were used to improve Raman signal intensity reading on highly fluorescent molecules. This instrument will be used to measure vibrational and electronic spectra of biological molecules.

Recent developments in white light emitting diodes

NASA Astrophysics Data System (ADS)

Lohe, P. P.; Nandanwar, D. V.; Belsare, P. D.; Moharil, S. V.

2018-05-01

In the recent years solid state lighting based on LEDs has revolutionized lighting technology. LEDs have many advantages over the conventional lighting based on fluorescent and incandescent lamps such as mercury free, high conversion efficiency of electrical energy into light, long lifetime reliability and ability to use with many types of devices. LEDs have emerged as a new potentially revolutionary technology that could save up to half of energy used for lighting applications. White LEDs would be the most important light source in the future, so much so that this aspect had been highlighted by the Nobel committee during the award of 2014 Nobel Prize for Physics. Recent advancement in the fabrication of GaN chip capable of emitting in blue and near UV region paved way for fabrication of white LED lamps. Mainly there are two approaches used for preparing white emitting solid state lamp. In the first approach blue light (λ=450 nm) emitted from the InGaN LED chip is partially absorbed by the YAG:Ce3+ phosphor coated on it and re-emitted as yellow fluorescence. A white light can be generated by the combination of blue + yellow emission bands. These lamps are already available. But they are suffering from major drawback that their Colour Rendering Index (CRI) is low. In the second approach, white LEDs are made by coating near ultraviolet emitting (360 to 410nm) LED with a mixture of high efficiency red, green and blue emitting phosphors, analogous to the fluorescent lamp. This method yields lamps with better color rendition. Addition of a yellow emitting phosphor improves CRI further. However conversion efficiency is compromised to some extent. Further the cost of near UV emitting chip is very high compared to blue emitting chips. Thus cost and light output wise, near UV chips are much inferior to blue chips. Recently some rare earth activated oxynitrides, silicates, fluorides have emerged as an important family of luminescent materials for white LED application because they can emit visible light strongly under blue light irradiation. These are chemically, thermally and mechanically stable materials with high efficiency to down convert blue radiation into green and red. Efficient white light can be generated by coating these phosphors on blue LED.CRI of white emitting LED lamp can be improved significantly if green and red emitting phosphors are coated on efficient blue emitting LED chips. In this approach CRI will be maintained if appropriate combination of red, green along with blue emission is used. This article reviews some recent developments in phosphors for white light emitting diodes.

The Novel Multiple Inner Primers-Loop-Mediated Isothermal Amplification (MIP-LAMP) for Rapid Detection and Differentiation of Listeria monocytogenes.

PubMed

Wang, Yi; Wang, Yan; Ma, Aijing; Li, Dongxun; Luo, Lijuan; Liu, Dongxin; Hu, Shoukui; Jin, Dong; Liu, Kai; Ye, Changyun

2015-12-03

Here, a novel model of loop-mediated isothermal amplification (LAMP), termed multiple inner primers-LAMP (MIP-LAMP), was devised and successfully applied to detect Listeria monocytogenes. A set of 10 specific MIP-LAMP primers, which recognized 14 different regions of target gene, was designed to target a sequence in the hlyA gene. The MIP-LAMP assay efficiently amplified the target element within 35 min at 63 °C and was evaluated for sensitivity and specificity. The templates were specially amplified in the presence of the genomic DNA from L. monocytogenes. The limit of detection (LoD) of MIP-LAMP assay was 62.5 fg/reaction using purified L. monocytogenes DNA. The LoD for DNA isolated from serial dilutions of L. monocytogenes cells in buffer and in milk corresponded to 2.4 CFU and 24 CFU, respectively. The amplified products were analyzed by real-time monitoring of changes in turbidity, and visualized by adding Loop Fluorescent Detection Reagent (FD), or as a ladder-like banding pattern on gel electrophoresis. A total of 48 pork samples were investigated for L. monocytogenes by the novel MIP-LAMP method, and the diagnostic accuracy was shown to be 100% when compared to the culture-biotechnical method. In conclusion, the MIP-LAMP methodology was demonstrated to be a reliable, sensitive and specific tool for rapid detection of L. monocytogenes strains.

A comparison between the effect of fluorescent lamps and quartz halogen incandescent filament lamps on the treatment of hyperbilirobinemia in newborns with the gestational age of 35 weeks or more.

PubMed

Sadeghnia, Alireza; Ganji, Masoud; Armanian, Amir Mohammad

2014-09-01

Icter is the most prevalent disease in newborns. Although most of the newborns affiliated with this seem healthy in other aspects, there is always a fear for toxic complication of indirect hyperbilirobinemia in the central nervous system. Nowadays phototherapy is the method of decreasing (or avoidance of increase) of total serum bilirobin (TSB) and it is also used widely in neonatal health care centers according to the availably of equipment, but without any defined standard. In this study, two light sources, quarts halogen incandescent filament lamp (QHIFL) and fluorescent lamp (FL) are compared with each other to find out which method is more useful and efficient. This study is a randomized controlled trial done on 25 newborns with gestational age of 35 weeks or more, with newborn's icter in the 1(st) week after birth, at Isfahan Behesti Hospital, February 2012 to March 2013. A group of these newborns was treated with FL and the other with QHIFL and they all were controlled and tested according to their level of TSB at the beginning of phototherapy, at 8(th), 12(th), and 24(th) h of treatment and at discharge. The data from the study was analyzed by IBM SPSS Statistics Version 21. According to the findings, the level of TSB before and 8 h after the intervention had no significant difference among the groups. However, at 16(th) and 24(th) h of treatment, the TSB level was lower in the FL group and this difference was meaningful (P = 0.002 and P = 0.013 respectively). Furthermore the duration of the treatment was significantly shorter in FL group meaningfully (P = 0.047). According to the findings of this study, the technology used in QHIFL cannot show the capabilities of the FLs. However, more studies are needed to confirm the findings of this study are universal.

Specific detection of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella from single vegetative cells by a loop-mediated isothermal amplification method.

PubMed

Nagai, Satoshi; Itakura, Shigeru

2012-09-01

In this study, we succeeded in developing a loop-mediated isothermal amplification (LAMP) method that enables sensitive and specific detection of the toxic marine dinoflagellates Alexandrium tamarense and Alexandrium catenella from single cells of both laboratory cultures and naturally blooming cells within 25 min, by monitoring the turbidimeter from the start of the LAMP reaction. The fluorescence intensity was strong enough to allow discrimination between positive and negative results by naked eye under a UV lamp, even in amplified samples from a single cell, by using the LAMP method. Unambiguous detection by naked eye was possible even in half the volume of LAMP cocktail recommended by the manufacturer, suggesting the potential to significantly reduce the cost of Alexandrium monitoring. Therefore, we can conclude that this method is one of the most convenient, sensitive, and cost-effective molecular tools for Alexandrium monitoring. Copyright © 2012 Elsevier B.V. All rights reserved.

An Experimental Study of the Fluorescence Spectrum of Cesium Atoms in the Presence of a Buffer Gas

NASA Astrophysics Data System (ADS)

Davydov, V. G.; Kulyasov, V. N.

2018-01-01

A direct experiment is performed to determine the quantum efficiency of a cesium fluorescence filter. The fluorescence spectra of cesium atoms are recorded under excitation of the upper states of the second resonance doublet with a Bell-Bloom cesium lamp. Introduction of different noble gases into the cell with cesium leads to the appearance of additional fluorescence photons. It is found that a fluorescence filter based on atomic cesium vapor with addition of helium in the working cell has the highest efficiency and response rate of all known fluorescence filters based on alkali-metal atomic vapors.

Light Spectral Quality Effects on the Growth of Potato (Solanum Tuberosum L.) Nodal Cutttings in Vitro

NASA Technical Reports Server (NTRS)

Wilson, Deborah A.; Weigel, Russell, C.; Wheeler, Raymond M.; Sager, John C.

1993-01-01

The effects of light spectral quality on the growth of in vitro nodal cutting of potato (Solanum tuberosum) cultivars Norland, Superior, Kennebec, and Denali were examined. The different light spectra were provided by Vita-Lite fluorescent (VF) (a white light control), blue fluorescent (BF), red fluorescent (RF), low-pressure sodium (LPS), and a combination of low-pressure sodium plus cool-white fluorescent lamp (LPS/CWF). Results suggested that shoot morphologic development of in vitro grown potato plants can be controlled by controlling irradiant spectral quality.

Xenon lighting adjusted to plant requirements

NASA Technical Reports Server (NTRS)

Koefferlein, M.; Doehring, T.; Payer, Hans D.; Seidlitz, H. K.

1994-01-01

Xenon lamps are available as low and high power lamps with relatively high efficiency and a relatively long lifetime up to several thousand hours. Different construction types of short-arc and long-arc lamps permit a good adaptation to various applications in projection and illumination techniques without substantial changes of the spectral quality. Hence, the xenon lamp was the best choice for professional technical purposes where high power at simultaneously good spectral quality of the light was required. However, technical development does not stand still. Between the luminous efficacy of xenon lamps of 25-50 lm/W and the theoretical limit for 'white light' of 250 lm/W is still much room for improvement. The present development mainly favors other lamp types, like metal halide lamps and fluorescent lamps for commercial lighting purposes. The enclosed sections deal with some of the properties of xenon lamps relevant to plant illumination; particularly the spectral aspects, the temporal characteristics of the emission, and finally the economy of xenon lamps will be addressed. Due to radiation exceeding the natural global radiation in both the ultraviolet (UV) and the infrared (IR) regions, filter techniques have to be included into the discussion referring to the requirements of plant illumination. Most of the presented results were obtained by investigations in the GSF phytotron or in the closed Phytocell chambers of the University of Erlangen. As our experiences are restricted to area plant illumination rather than spot lights our discussion will concentrate on low pressure long-arc xenon lamps which are commonly used for such plant illuminations. As the spectral properties of short-arc lamps do not differ much from those of long-arc lamps most of our conclusions will be valid for high pressure xenon lamps too. These lamps often serve as light sources for small sun simulators and for monochromators which are used for action spectroscopy of plant responses.

Endoscopic tissue autofluorescence measurements in the upper aerodigestive tract and the bronchi

NASA Astrophysics Data System (ADS)

Braichotte, Daniel; Wagnieres, Georges A.; Monnier, Philippe; Savary, Jean-Francois; Bays, Roland; van den Bergh, Hubert; Chatelain, Andre

1991-11-01

A single multimode optical fiber is used to excite and collect tissue autofluorescence as well as the fluorescence of an IV-injected fluorescent tumor marker. Measurements of the relative fluorescence intensity of a tumor marker as a function of the time after IV injection permit measurement of the kinetics of this substance in tumor, normal tissue, and skin. The authors believe that these are the first measurements of this kind in patients. Furthermore, the autofluorescence spectrum generated at several excitation wavelengths in different tissues is compared, for instance in the oesophagus, the bronchi, and the tongue. The measuring system is based on an optical multichannel analyzer which measures the fluorescence excited by monochromatic radiation from a spectrally filtered Xe lamp. A correlation between the observed pharmacokinetics and tumor properties like the degree of vascularization is of fundamental importance for each selected tumor marker. Also, the results of these measurements are used for the optical detection of tumors.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tuenge, Jason R.; Hollomon, Brad; Dillon, Heather E.

This report covers the third part of a larger U.S. Department of Energy (DOE) project to assess the life-cycle environmental and resource impacts in the manufacturing, transport, use, and disposal of light-emitting diode (LED) lighting products in relation to incumbent lighting technologies. All three reports are available on the DOE website (www.ssl.energy.gov/tech_reports.html). • Part 1: Review of the Life-Cycle Energy Consumption of Incandescent, Compact Fluorescent and LED Lamps; • Part 2: LED Manufacturing and Performance; • Part 3: LED Environmental Testing. Parts 1 and 2 were published in February and June 2012, respectively. The Part 1 report included a summarymore » of the life-cycle assessment (LCA) process and methodology, provided a literature review of more than 25 existing LCA studies of various lamp types, and performed a meta-analysis comparing LED lamps with incandescent and compact fluorescent lamps (CFLs). Drawing from the Part 1 findings, Part 2 performed a more detailed assessment of the LED manufacturing process and used these findings to provide a comparative LCA taking into consideration a wider range of environmental impacts. Both reports concluded that the life-cycle environmental impact of a given lamp is dominated by the energy used during lamp operation—the upstream generation of electricity drives the total environmental footprint of the product. However, a more detailed understanding of end-of-life disposal considerations for LED products has become increasingly important as their installation base has grown. The Part 3 study (reported herein) was undertaken to augment the LCA findings with chemical analysis of a variety of LED, CFL, and incandescent lamps using standard testing procedures. A total of 22 samples, representing 11 different models, were tested to determine whether any of 17 elements were present at levels exceeding California or Federal regulatory thresholds for hazardous waste. Key findings include: • The selected models were generally found to be below thresholds for Federally regulated elements; • All CFLs and LED lamps and most incandescent lamps exceeded California thresholds for Copper; • Most CFL samples exceeded California thresholds for Antimony and Nickel, and half of the LED samples exceeded California thresholds for Zinc; • The greatest contributors were the screw bases, drivers, ballasts, and wires or filaments; • Overall concentrations in LED lamps were comparable to cell phones and other types of electronic devices, and were generally attributable to components other than the internal LED light sources; • Although the life-cycle environmental impact of the LED lamps is favorable when compared to CFLs and incandescent lamps, recycling will likely gain importance as consumer adoption increases. This study was exploratory in nature and was not intended to provide a definitive indication of regulatory compliance for any specific lamp model or technology. Further study would be needed to more broadly characterize the various light source technologies; to more accurately and precisely characterize a specific model; or to determine whether product redesign would be appropriate.« less

Determining heavy metals in spent compact fluorescent lamps (CFLs) and their waste management challenges: Some strategies for improving current conditions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Taghipour, Hassan, E-mail: hteir@yahoo.com; Amjad, Zahra; Jafarabadi, Mohamad Asghari

2014-07-15

Highlights: • Heavy metals in spent compact fluorescent lamps (CFLs) determined. • Current waste management condition of CFLs in Iran assessed. • Currently, waste of CFLs is disposed by municipal waste stream in waste landfills. • We propose extended producer responsibility (EPR) for CFLs waste management. - Abstract: From environmental viewpoint, the most important advantage of compact fluorescent lamps (CFLs) is reduction of green house gas emissions. But their significant disadvantage is disposal of spent lamps because of containing a few milligrams of toxic metals, especially mercury and lead. For a successful implementation of any waste management plan, availability ofmore » sufficient and accurate information on quantities and compositions of the generated waste and current management conditions is a fundamental prerequisite. In this study, CFLs were selected among 20 different brands in Iran. Content of heavy metals including mercury, lead, nickel, arsenic and chromium was determined by inductive coupled plasma (ICP). Two cities, Tehran and Tabriz, were selected for assessing the current waste management condition of CFLs. The study found that waste generation amount of CFLs in the country was about 159.80, 183.82 and 153.75 million per year in 2010, 2011 and 2012, respectively. Waste generation rate of CFLs in Iran was determined to be 2.05 per person in 2012. The average amount of mercury, lead, nickel, arsenic and chromium was 0.417, 2.33, 0.064, 0.056 and 0.012 mg per lamp, respectively. Currently, waste of CFLs is disposed by municipal waste stream in waste landfills. For improving the current conditions, we propose by considering the successful experience of extended producer responsibility (EPR) in other electronic waste management. The EPR program with advanced recycling fee (ARF) is implemented for collecting and then recycling CFLs. For encouraging consumers to take the spent CFLs back at the end of the products’ useful life, a proportion of ARF (for example, 50%) can be refunded. On the other hand, the government and Environmental Protection Agency should support and encourage recycling companies of CFLs both technically and financially in the first place.« less

Tuning the white light spectrum of light emitting diode lamps to reduce attraction of nocturnal arthropods

PubMed Central

Longcore, Travis; Aldern, Hannah L.; Eggers, John F.; Flores, Steve; Franco, Lesly; Hirshfield-Yamanishi, Eric; Petrinec, Laina N.; Yan, Wilson A.; Barroso, André M.

2015-01-01

Artificial lighting allows humans to be active at night, but has many unintended consequences, including interference with ecological processes, disruption of circadian rhythms and increased exposure to insect vectors of diseases. Although ultraviolet and blue light are usually most attractive to arthropods, degree of attraction varies among orders. With a focus on future indoor lighting applications, we manipulated the spectrum of white lamps to investigate the influence of spectral composition on number of arthropods attracted. We compared numbers of arthropods captured at three customizable light-emitting diode (LED) lamps (3510, 2704 and 2728 K), two commercial LED lamps (2700 K), two commercial compact fluorescent lamps (CFLs; 2700 K) and a control. We configured the three custom LEDs to minimize invertebrate attraction based on published attraction curves for honeybees and moths. Lamps were placed with pan traps at an urban and two rural study sites in Los Angeles, California. For all invertebrate orders combined, our custom LED configurations were less attractive than the commercial LED lamps or CFLs of similar colour temperatures. Thus, adjusting spectral composition of white light to minimize attracting nocturnal arthropods is feasible; not all lights with the same colour temperature are equally attractive to arthropods. PMID:25780237

Max Tech and Beyond: High-Intensity Discharge Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scholand, Michael

High-intensity discharge (HID) lamps are most often found in industrial and commercial applications, and are the light source of choice in street and area lighting, and sports stadium illumination. HID lamps are produced in three types - mercury vapor (MV), high pressure sodium (HPS) and metal halide (MH). Of these, MV and MH are considered white-light sources (although the MV exhibits poor color rendering) and HPS produces a yellow-orange color light. A fourth lamp, low-pressure sodium (LPS), is not a HID lamp by definition, but it is used in similar applications and thus is often grouped with HID lamps. Withmore » the notable exception of MV which is comparatively inefficient and in decline in the US from both a sales and installed stock point of view; HPS, LPS and MH all have efficacies over 100 lumens per watt. The figure below presents the efficacy trends over time for commercially available HID lamps and LPS, starting with MV and LPS in 1930's followed by the development of HPS and MH in the 1960's. In HID lamps, light is generated by creating an electric arc between two electrodes in an arc tube. The particles in the arc are partially ionized, making them electrically conductive, and a light-emitting 'plasma' is created. This arc occurs within the arc tube, which for most HID lamps is enclosed within an evacuated outer bulb that thermally isolates and protects the hot arc tube from the surroundings. Unlike a fluorescent lamp that produces visible light through down-converting UV light with phosphors, the arc itself is the light source in an HID lamp, emitting visible radiation that is characteristic of the elements present in the plasma. Thus, the mixture of elements included in the arc tube is one critical factor determining the quality of the light emitted from the lamp, including its correlated color temperature (CCT) and color rendering index (CRI). Similar to fluorescent lamps, HID lamps require a ballast to start and maintain stable operating conditions, and this necessitates additional power beyond that used by the lamp itself. HID lamps offer important advantages compared to other lighting technologies, making them well suited for certain applications. HID lamps can be very efficient, have long operating lives, are relatively temperature-insensitive and produce a large quantity of light from a small package. For these reasons, HID lamps are often used when high levels of illumination are required over large areas and where operating and maintenance costs must be kept to a minimum. Furthermore, if the installation has a significant mounting height, high-power HID lamps can offer superior optical performance luminaires, reducing the number of lamps required to illuminate a given area. The indoor environments best suited to HID lamps are those with high ceilings, such as those commonly found in industrial spaces, warehouses, large retail spaces, sports halls and large public areas. Research into efficacy improvements for HID lighting technologies has generally followed market demand for these lamps, which is in decline for MV and LPS, has reached a plateau for HPS and is growing for MH. Several manufacturers interviewed for this study indicated that although solid-state lighting was now receiving the bulk of their company's R&D investment, there are still strong HID lamp research programs, which concentrate on MH technologies, with some limited amount of investment in HPS for specific niche applications (e.g., agricultural greenhouses). LPS and MV lamps are no longer being researched or improved in terms of efficacy or other performance attributes, although some consider MH HID lamps to be the next-generation MV lamp. Thus, the efficacy values of commercially available MV, LPS and HPS lamps are not expected to increase in the next 5 to 10 years. MH lamps, and more specifically, ceramic MH lamps are continuing to improve in efficacy as well as light quality, manufacturability and lamp life. Within an HID lamp, the light-producing plasma must be heated to sufficiently high temperatures to achieve high efficiencies, without melting the electrodes or altering the operating conditions of the lamp. The research in ceramic MH has focused on the arc tube, the electrodes and the plasma, resulting in an innovation announced by Philips Lighting in 2009 called the 'unsaturated lamp.' The unsaturated lamp addresses a problem experienced by standard ceramic MH lamps where a pool of liquid salt develops in the arc tube while the lamp is operating. This pool of liquid salt limits the light characteristics of the lamp such as the efficacy and color quality, and reduces lamp lifetime. By making modifications to the arc tube, the pressure and the operating temperature, the unsaturated ceramic MH lamp resolves this issue by keeping all the halide salts in the gaseous phase, even while the lamp is dimming (down to 50%).« less

Approach to the Lighting Energy Savings in Japan for Global Climate Change Prevention

NASA Astrophysics Data System (ADS)

Hanada, Teizo

This report was presented as an invited speech at “the First Lighting Symposium of China, Japan and Korea” held in Beijing on October 24, 2008. The reporter has introduced JELMA's proposal for energy saving in lighting, and explained purpose of their activities. Latest activities for replacing incandescent lamps to CFLi and its recent results are also reported. Japanese next big target for lighting energy saving is to replace the conventional fluorescent lamps to Hf-FLs.

Prime-Color Concept: Lighting for the Future

ERIC Educational Resources Information Center

Modern Schools, 1976

1976-01-01

A major technological breakthrough--the isolation and then combination of narrow bands of blue-violet, pure green, and orange-red energy--has resulted in a highly efficient white fluorescent lamp. (Author/MLF)

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

The RE Light process is a process that recycles fluorescent lamps for their rare earth and mercury. There is a limited U.S. market for recycled rare earth materials and current rare earth prices make domestic production cost-prohibitive.

The role of ultraviolet-A reflectance and ultraviolet-A induced fluorescence in the appearance of budgerigar plumage: insights from spectrofluorometry and reflectance spectrophotometry.

PubMed Central

Pearn, Sophie M; Bennett, Andrew T D; Cuthill, Innes C

2003-01-01

Fluorescence has so far been found in 52 parrot species when illuminated with ultraviolet-A (UVA) 'black' lamps, and two attempts have been made to determine whether such fluorescence plays any role in sexual signalling. However, the contribution of the reflectance versus fluorescence to the total radiance from feathers, even in the most studied species to date (budgerigars), is unclear. Nor has the plumage of this study species been systematically assessed to determine the distribution of fluorescent patches. We therefore used spectrofluorometry to determine which areas of budgerigars fluoresce and the excitation and emission spectra involved; this is the first time that such a technique has been applied to avian plumage. We found that both the yellow crown and (normally hidden) white downy chest feathers exhibit strong UVA-induced fluorescence, with peak emissions at 527 nm and 436 nm, respectively. Conversely, the bright-green chest and dark-blue tail feathers do not fluoresce. When comparing reflectance spectra (400-700 nm) from the yellow crown using illuminants with a proportion of UVA comparable to daylight, and illuminants with all UVA removed, no measurable difference resulting from fluorescence was found. This suggests that under normal daylight the contribution of fluorescence to radiance is probably trivial. Furthermore, these spectra revealed that males had fluorescent crowns with substantially higher reflectance than those of females, in both the UV waveband and at longer wavelengths. Reflectance spectrophotometry was also performed on a number of live wild-type male budgerigars to investigate the chromatic contrast between the different plumage areas. This showed that many plumage regions are highly UV-reflective. Overall our results suggest that rapid surveys using UVA black lamps may overestimate the contribution of fluorescence to plumage coloration, and that any signalling role of fluorescence emissions, at least from the yellow crown of budgerigars, may not be as important as previously thought. PMID:12737665

Dual lanthanide-doped complexes: the development of a time-resolved ratiometric fluorescent probe for anthrax biomarker and a paper-based visual sensor.

PubMed

Wang, Qi-Xian; Xue, Shi-Fan; Chen, Zi-Han; Ma, Shi-Hui; Zhang, Shengqiang; Shi, Guoyue; Zhang, Min

2017-08-15

In this work, a novel time-resolved ratiometric fluorescent probe based on dual lanthanide (Tb: terbium, and Eu: europium)-doped complexes (Tb/DPA@SiO 2 -Eu/GMP) has been designed for detecting anthrax biomarker (dipicolinic acid, DPA), a unique and major component of anthrax spores. In such complexes-based probe, Tb/DPA@SiO 2 can serve as a stable reference signal with green fluorescence and Eu/GMP act as a sensitive response signal with red fluorescence for ratiometric fluorescent sensing DPA. Additionally, the probe exhibits long fluorescence lifetime, which can significantly reduce the autofluorescence interferences from biological samples by using time-resolved fluorescence measurement. More significantly, a paper-based visual sensor for DPA has been devised by using filter paper embedded with Tb/DPA@SiO 2 -Eu/GMP, and we have proved its utility for fluorescent detection of DPA, in which only a handheld UV lamp is used. In the presence of DPA, the paper-based visual sensor, illuminated by a handheld UV lamp, would result in an obvious fluorescence color change from green to red, which can be easily observed with naked eyes. The paper-based visual sensor is stable, portable, disposable, cost-effective and easy-to-use. The feasibility of using a smartphone with easy-to-access color-scanning APP as the detection platform for quantitative scanometric assays has been also demonstrated by coupled with our proposed paper-based visual sensor. This work unveils an effective method for accurate, sensitive and selective monitoring anthrax biomarker with backgroud-free and self-calibrating properties. Copyright © 2017 Elsevier B.V. All rights reserved.

Light emitting diode excitation emission matrix fluorescence spectroscopy.

PubMed

Hart, Sean J; JiJi, Renée D

2002-12-01

An excitation emission matrix (EEM) fluorescence instrument has been developed using a linear array of light emitting diodes (LED). The wavelengths covered extend from the upper UV through the visible spectrum: 370-640 nm. Using an LED array to excite fluorescence emission at multiple excitation wavelengths is a low-cost alternative to an expensive high power lamp and imaging spectrograph. The LED-EEM system is a departure from other EEM spectroscopy systems in that LEDs often have broad excitation ranges which may overlap with neighboring channels. The LED array can be considered a hybrid between a spectroscopic and sensor system, as the broad LED excitation range produces a partially selective optical measurement. The instrument has been tested and characterized using fluorescent dyes: limits of detection (LOD) for 9,10-bis(phenylethynyl)-anthracene and rhodamine B were in the mid parts-per-trillion range; detection limits for the other compounds were in the low parts-per-billion range (< 5 ppb). The LED-EEMs were analyzed using parallel factor analysis (PARAFAC), which allowed the mathematical resolution of the individual contributions of the mono- and dianion fluorescein tautomers a priori. Correct identification and quantitation of six fluorescent dyes in two to six component mixtures (concentrations between 12.5 and 500 ppb) has been achieved with root mean squared errors of prediction (RMSEP) of less than 4.0 ppb for all components.

Modelling of the mercury loss in fluorescent lamps under the influence of metal oxide coatings

NASA Astrophysics Data System (ADS)

Santos Abreu, A.; Mayer, J.; Lenk, D.; Horn, S.; Konrad, A.; Tidecks, R.

2016-11-01

The mercury transport and loss mechanisms in the metal oxide coatings of mercury low pressure discharge fluorescent lamps have been investigated. An existing model based on a ballistic process is discussed in the context of experimental mercury loss data. Two different approaches to the modeling of the mercury loss have been developed. The first one is based on mercury transition rates between the plasma, the coating, and the glass without specifying the underlying physical processes. The second one is based on a transport process driven by diffusion and a binding process of mercury reacting to mercury oxide inside the layers. Moreover, we extended the diffusion based model to handle multi-component coatings. All approaches are applied to describe mercury loss experiments under the influence of an Al 2 O 3 coating.

Synthesis and luminescence of Ca 4YO(BO 3) 3:Eu 3+ for fluorescent lamp application

NASA Astrophysics Data System (ADS)

Kuo, Te-Wen; Chen, Teng-Ming

2010-07-01

The red-emitting Ca 4YO(BO 3) 3:Eu 3+ phosphor has been prepared at 1200 °C by the simple solid-state reaction. This preparation temperature is much lower than Y 2O 3:Eu 3+ (1400-1500 °C) for conventional solid-state reaction method. In particular, the complete process to produce high-quality phosphor particles was carried out through the single-step heat treatment of the mixture of corresponding oxide-type metal sources. For this material, the XRD, PL, PL excitation (PLE) and SEM features have also been investigated. The X-ray diffraction data indicate that pure phase of Ca 4YO(BO 3) 3:Eu 3+ can be successfully obtained. Among the different emission transitions 5D 0 → 7F J=0, 1, 2, 3, 4 of this phosphor, one particular transition ( 5D 0 → 7F 2) at 610 nm has been found. Besides carrying out these essential measurements, we have also made an attempt to observe a strong red emission performance displayed by this phosphor for use as coating material on compact fluorescent lamps (CFLs). The results clearly indicate that the life time based on Ca 4YO(BO 3) 3:Eu 3+ was found to be much longer than that using Y 2O 3:Eu 3+. The good performances of the CFLs demonstrate that this phosphor may be suitable for application on short ultraviolet fluorescent lamp.

Comparing colour discrimination and proofreading performance under compact fluorescent and halogen lamp lighting.

PubMed

Mayr, Susanne; Köpper, Maja; Buchner, Axel

2013-01-01

Legislation in many countries has banned inefficient household lighting. Consequently, classic incandescent lamps have to be replaced by more efficient alternatives such as halogen and compact fluorescent lamps (CFL). Alternatives differ in their spectral power distributions, implying colour-rendering differences. Participants performed a colour discrimination task - the Farnsworth-Munsell 100 Hue Test--and a proofreading task under CFL or halogen lighting of comparable correlated colour temperatures at low (70 lx) or high (800 lx) illuminance. Illuminance positively affected colour discrimination and proofreading performance, whereas the light source was only relevant for colour discrimination. Discrimination was impaired with CFL lighting. There were no differences between light sources in terms of self-reported physical discomfort and mood state, but the majority of the participants correctly judged halogen lighting to be more appropriate for discriminating colours. The findings hint at the colour-rendering deficiencies associated with energy-efficient CFLs. In order to compare performance under energy-efficient alternatives of classic incandescent lighting, colour discrimination and proofreading performance was compared under CFL and halogen lighting. Colour discrimination was impaired under CFLs, which hints at the practical drawbacks associated with the reduced colour-rendering properties of energy-efficient CFLs.

A remote operating slit lamp microscope system. Development and its utility in ophthalmologic examinations.

PubMed

Tanabe, N; Go, K; Sakurada, Y; Imasawa, M; Mabuchi, F; Chiba, T; Abe, K; Kashiwagi, K

2011-01-01

To develop a remote-operating slit lamp microscope system (the remote slit lamp) as the core for highly specialized ophthalmology diagnoses, and to compare the utility of this system with the conventional slit lamp microscope system (the conventional slit lamp) in making a diagnosis. The remote slit lamp system was developed. Three factors were evaluated in comparison to the conventional slit lamp. The ability to acquire skills was investigated using a task loading system among specialists and residents in ophthalmology. Participants repeated a task up to ten times and the time required for each task was analyzed. The consistency of the two systems in making a diagnosis was investigated using eyes of patients with ocular diseases as well as healthy volunteers. The remote slit lamp is composed of a patient's unit and ophthalmologist's unit connected by high-speed internet. The two units share images acquired by the slit lamp in addition to the images and voices of patients and ophthalmologists. Both ophthalmology specialists and residents could minimize the completion times after several trials. The remote slit lamp took more time than the conventional slit lamp. Both systems showed a high consistency in evaluations among eyes with healthy eyes or those with ocular diseases. The remote slit lamp has a similar diagnostic ability, but required more examination time in comparison to the conventional slit lamp. The currently developed remote slit lamp has the potential to be employed for tele-medicine purposes in the field of ophthalmology.

Rapid detection of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) by loop-mediated isothermal amplification (LAMP).

PubMed

Bhat, A I; Siljo, A; Deeshma, K P

2013-10-01

The loop-mediated isothermal amplification (LAMP) assay for Piper yellow mottle virus and the reverse transcription (RT) LAMP assay for Cucumber mosaic virus each consisted of a set of five primers designed against the conserved sequences in the viral genome. Both RNA and DNA isolated from black pepper were used as a template for the assay. The results were assessed visually by checking turbidity, green fluorescence and pellet formation in the reaction tube and also by gel electrophoresis. The assay successfully detected both viruses in infected plants whereas no cross-reactions were recorded with healthy plants. Optimum conditions for successful amplification were determined in terms of the concentrations of magnesium sulphate and betaine, temperature, and duration. The detection limit for both LAMP and RT-LAMP was up to 100 times that for conventional PCR and up to one-hundredth of that for real-time PCR. The optimal conditions arrived at were validated by testing field samples of infected vines of three species from different regions. Copyright © 2013 Elsevier B.V. All rights reserved.

Direct duplex real-time loop mediated isothermal amplification assay for the simultaneous detection of cow and goat species origin of milk and yogurt products for field use.

PubMed

Kim, Mi-Ju; Kim, Hae-Yeong

2018-04-25

A multiple loop-mediated isothermal amplification (LAMP) method was developed to detect cow and goat milk in the field using a portable fluorescence device. For rapid on-site detection, this duplex LAMP assay was used in combination with direct amplification, without DNA extraction. The cow- and goat-specific LAMP primer sets were designed based on the mitochondrial cytochrome b gene, and showed specificity against 13 other animal species in the reactions. The sensitivity of the duplex LAMP assay for cow and goat was 0.1 and 1 pg, respectively. The detection limit for both target species in milk mixtures was 2%. This assay successfully amplified and identified the two target species in 24 samples of commercial milk and yogurt products, with 30 min sampling-to-result analysis time. Therefore, this direct duplex real-time LAMP assay is useful for on-site simultaneous detection of cow and goat milk in commercial products, a capability needed to confirm accurate labeling. Copyright © 2017 Elsevier Ltd. All rights reserved.

Studies on activated cytostatic fluorouracil as photosensitizer: to use in eye tumor treatment

NASA Astrophysics Data System (ADS)

Pascu, Mihail-Lucian; Carstocea, Benone D.; Brezeanu, Mihail; Voicu, Letitia; Staicu, Angela; Gazdaru, Doina M.; Pascu, Ruxandra A.

2004-09-01

Hydroxypyrimidine 5-fluorouracil (5-FU) belongs to the cytostatics group known as antimetabolites. The effect of UV irradiation on 5-FU was investigated by absorption and fluorescence spectroscopy. The study of the photosensitizer properties of 5-FU was made since their effects could be enhanced by exposure to UV radiation at different doses. Solutions 2.5x10-4M in natural saline water (0.8% NaCl), irradiated with optical beams emitted by N2 laser and UV Hg classic lamp, were used. The 5-FU was chosen due to its strong absorption along a large spectral range which makes possible the fluorescence excitation in UV. The absorption spectra exhibit bands between 250 - 450 nm. The emission fluorescence was measured in the 400-550 nm spectral range, with λex=320 and 350 nm for samples irradiated with Hg lamp and with λex=360 nm for samples irradiated with N2 laser. The excitation fluorescence was measured in the spectral range 200-400 nm, with λem=440 nm for samples irradiated with N2 laser. The spectra reveal a fluorescence enhancement with the exposure time, with a maximum at 3 min due to the transformation of 5-FU molecule into a fluorescent tautomeric form. The destruction more rapid than usual of the neovascularisation was observed for conjunctive of rabbit eyes, when they are impregnated with 5-FU solution and exposed to incoherent UV and visible light.

One simple DNA extraction device and its combination with modified visual loop-mediated isothermal amplification for rapid on-field detection of genetically modified organisms.

PubMed

Zhang, Miao; Liu, Yinan; Chen, Lili; Quan, Sheng; Jiang, Shimeng; Zhang, Dabing; Yang, Litao

2013-01-02

Quickness, simplicity, and effectiveness are the three major criteria for establishing a good molecular diagnosis method in many fields. Herein we report a novel detection system for genetically modified organisms (GMOs), which can be utilized to perform both on-field quick screening and routine laboratory diagnosis. In this system, a newly designed inexpensive DNA extraction device was used in combination with a modified visual loop-mediated isothermal amplification (vLAMP) assay. The main parts of the DNA extraction device included a silica gel membrane filtration column and a modified syringe. The DNA extraction device could be easily operated without using other laboratory instruments, making it applicable to an on-field GMO test. High-quality genomic DNA (gDNA) suitable for polymerase chain reaction (PCR) and isothermal amplification could be quickly isolated from plant tissues using this device within 15 min. In the modified vLAMP assay, a microcrystalline wax encapsulated detection bead containing SYBR green fluorescent dye was introduced to avoid dye inhibition and cross-contaminations from post-LAMP operation. The system was successfully applied and validated in screening and identification of GM rice, soybean, and maize samples collected from both field testing and the Grain Inspection, Packers, and Stockyards Administration (GIPSA) proficiency test program, which demonstrated that it was well-adapted to both on-field testing and/or routine laboratory analysis of GMOs.

Stem loop-mediated isothermal amplification test: comparative analysis with classical LAMP and PCR in detection of Entamoeba histolytica in Kenya.

PubMed

Mwendwa, Fridah; Mbae, Cecilia K; Kinyua, Johnson; Mulinge, Erastus; Mburugu, Gitonga Nkanata; Njiru, Zablon K

2017-03-31

Entamoeba histolytica, the causative agent for amoebiasis is a considerable burden to population in the developing countries where it accounts for over 50 million infections. The tools for detection of amoebiasis are inadequate and diagnosis relies on microscopy which means a significant percent of cases remain undiagnosed. Moreover, tests formats that can be rapidly applied in rural endemic areas are not available. In this study, a loop-mediated isothermal test (LAMP) based on 18S small subunit ribosomal RNA gene was designed with extra reaction accelerating primers (stem primers) and compared with the published LAMP and PCR tests in detection of E. histolytica DNA in clinical samples. The stem LAMP test indicated shorter time to results by an average 11 min and analytical sensitivity of 10 -7 (~30 pg/ml) compared to the standard LAMP and PCR which showed sensitivities levels of 10 -5 (~3 ng/ml) and 10 -4 (~30 ng/ml) respectively using tenfold serial dilution of DNA. In the analysis of clinical specimens positive for Entamoeba spp. trophozoites and cysts using microscopy, the stem LAMP test detected E. histolytica DNA in 36/126, standard LAMP test 20/126 and PCR 17/126 cases respectively. There was 100% agreement in detection of the stem LAMP test product using fluorescence of SYTO-9 dye in real time machine, through addition of 1/10 dilution of SYBR ® Green I and electrophoresis in 2% agarose gel stained with ethidium bromide. The stem LAMP test developed in this study indicates potential towards detection of E. histolytica.

The application of IS6110-baced loop-mediated isothermal amplification (LAMP) in the early diagnosis of tuberculous meningitis.

PubMed

Sun, Wen-Wen; Sun, Qin; Yan, Li-Ping; Zhang, Qing

2017-08-22

Here, we evaluated the potential activity of rapid Mycobacterium tuberculosis detection with loop-mediated isothermal amplification (LAMP), for the early diagnosis of tuberculous meningitis (TBM). Patients with suspected TBM from January 2014 to December 2015 were reviewed retrospectively. The cerebrospinalfluid(CSF) was collected. Acid-fast bacillus (AFB) staining, MGIT 960 culture, real-time fluorescent quantitative polymerase chain reaction (RTFQ PCR) and LAMP were performed. A total of 200 patients were included in the study. Of which, 172 of them were diagnosed with TBM (86.00%). The sensitivities of AFB staining, MGIT 960 culture, LAMP and RTFQ PCR for TBM diagnosis were 2.91% (5/172), 12.79% (22/172), 43.02% (74/172), and 34.30% (59/172), respectively. The sensitivity of LAMP for TBM was significantly higher than those of AFB staining and MGIT960 culture ( χ2 = 75.11, P < 0.001; χ2 = 43.88, P = 0.002). LAMP's sensitivity was however comparable to RTFQ PCR assay ( χ2 = 2.08, P = 0.130). The specificity, positive predictive value and negative predictive value of LAMP in the diagnosis of TBM were 92.86% (26/28), 97.37% (74/76) and 20.97 % (26/124), respectively. The overall consistency between LAMP and RTFQ PCR in the diagnosis of TBM was 88.5% (177/200), with Kappa value of 0.870. The consistency between LAMP and MGIT960 culture was 71% (142/200), with Kappa value of 0.730. Among all the methods, LAMP had high sensitivity, specificity and positive predictive value, showing high consistency with MGIT960 culture and RTFQ PCR.

Retail Lamps Study 3.1: Dimming, Flicker, and Power Quality Characteristics of LED A Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

Royer, Michael P.; Poplawski, Michael E.; Brown, Charles C.

2014-12-01

To date, all three reports in the retail lamps series have focused on basic performance parameters, such as lumen output, efficacy, and color quality. This report goes a step further, examining the photoelectric characteristics (i.e., dimming and flicker) of a subset of lamps from CALiPER Retails Lamps Study 3. Specifically, this report focuses on the dimming, power quality, and flicker characteristics of 14 LED A lamps, as controlled by four different retail-available dimmers. The results demonstrate notable variation across the various lamps, but little variation between the four dimmers. Overall, the LED lamps: ~tended to have higher relative light outputmore » compared to the incandescent and halogen benchmark at the same dimmer output signal (RMS voltage). The lamps’ dimming curves (i.e., the relationship between control signal and relative light output) ranged from linear to very similar to the square-law curve typical of an incandescent lamp. ~generally exhibited symmetrical behavior—the same dimming curve—when measured proceeding from maximum to minimum or minimum to maximum control signal. ~mostly dimmed below 10% of full light output, with some exceptions for specific lamp and dimmer combinations ~exhibited a range of flicker characteristics, with many comparing favorably to the level typical of a magnetically-ballasted fluorescent lamp through at least a majority of the dimming range. ~ always exceeded the relative (normalized) efficacy over the dimming range of the benchmark lamps, which rapidly decline in efficacy when they are dimmed. This report generally does not attempt to rank the performance of one product compared to another, but instead focuses on the collective performance of the group versus conventional incandescent or halogen lamps, the performance of which is likely to be the baseline for a majority of consumers. Undoubtedly, some LED lamps perform better—or more similar to conventional lamps—than others. Some perform desirably for one characteristic, but not others. Consumers (and specifiers) may have a hard time distinguishing better-performing lamps from one another; at this time, physical experimentation is likely the best evaluation tool.« less

Photosynthetically supplemental lighting for vegetable crop production with super-bright laser diode

NASA Astrophysics Data System (ADS)

Hu, Yongguang; Li, Pingping; Shi, Jintong

2007-02-01

Although many artificial light sources like high-pressure sodium lamp, metal halide lamp, fluorescent lamp and so on are commonly used in horticulture, they are not widely applied because of the disadvantages of unreasonable spectra, high cost and complex control. Recently new light sources of light-emitting diode (LED) and laser diode (LD) are becoming more and more popular in the field of display and illumination with the improvement of material and manufacturing, long life-span and increasingly low cost. A new type of super-bright red LD (BL650, central wavelength is 650 nm) was selected to make up of the supplemental lighting panel, on which LDs were distributed with regular hexagon array. Drive circuit was designed to power it and adjust light intensity. System performance including temperature rise and light intensity distribution under different vertical/horizontal distances were tested. Photosynthesis of sweet pepper and eggplant leaf under LD was measured with LI-6400 to show the supplemental lighting effects. The results show that LD system can supply the maximum light intensity of 180 μmol/m2 •s at the distance of 50 mm below the panel and the temperature rise is little within 1 °C. Net photosynthetic rate became faster when LD system increased light intensity. Compared with sunlight and LED supplemental lighting system, LD's promotion on photosynthesis is in the middle. Thus it is feasible for LD light source to supplement light for vegetable crops. Further study would focus on the integration of LD and other artificial light sources.

Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting

NASA Technical Reports Server (NTRS)

Brown, C. S.; Schuerger, A. C.; Sager, J. C.

1995-01-01

Light-emitting diodes (LEDs) are a potential irradiation source for intensive plant culture systems and photobiological research. They have small size, low mass, a long functional life, and narrow spectral output. In this study, we measured the growth and dry matter partitioning of 'Hungarian Wax' pepper (Capsicum annuum L.) plants grown under red LEDs compared with similar plants grown under red LEDs with supplemental blue or far-red radiation or under broad spectrum metal halide (MH) lamps. Additionally, we describe the thermal and spectral characteristics of these sources. The LEDs used in this study had a narrow bandwidth at half peak height (25 nm) and a focused maximum spectral output at 660 nm for the red and 735 nm for the far-red. Near infrared radiation (800 to 3000 nm) was below detection and thermal infrared radiation (3000 to 50,000 nm) was lower in the LEDs compared to the MH source. Although the red to far-red ratio varied considerably, the calculated phytochrome photostationary state (phi) was only slightly different between the radiation sources. Plant biomass was reduced when peppers were grown under red LEDs in the absence of blue wavelengths compared to plants grown under supplemental blue fluorescent lamps or MH lamps. The addition of far-red radiation resulted in taller plants with greater stem mass than red LEDs alone. There were fewer leaves under red or red plus far-red radiation than with lamps producing blue wavelengths. These results indicate that red LEDs may be suitable, in proper combination with other wavelengths of light, for the culture of plants in tightly controlled environments such as space-based plant culture systems.

Use of diffusive optical fibers for plant lighting

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kozai, T.; Kitaya, Y.; Fujiwara, K.

1994-12-31

Lighting is one of the most critical aspects in plant production and environmental research with plants. Much research has been repeated on the effect of light intensity, spectral distribution of light and lighting cycle, but comparatively little research done on the effect of lighting direction on the growth, development and morphology of plants. When plants are grown with lamps above, light is directed downward to the plants. Downward or overhead lighting is utilized in almost all cases. However, downward lighting does not always give the best result in terms of lighting efficiency, growth, development and morphology of plants. Kitaya etmore » al. (1988) developed a lighting system in which two rooting beds were arranged; one above and the other under fluorescent lamps. Lettuce plants grew normally in the lower bed and suspended upside-down under the upper bed. The lettuce plants suspended upside-down were given the light in upward direction (upward lighting). No significant difference in growth, development and morphology was found between the lettuce plants grown by the downward and upward lighting. Combining upward and downward lighting, improved spacing efficiency and reduced electricity cost per plant compared with conventional, downward lighting. From the above example, when designing a lighting system for plants with lamps more lighting direction should be considered. In the present study, a sideward lighting system was developed using diffusive optical fiber belts. More higher quality tissue-cultured transplants could be produced in reduced space with sideward lighting system than with a downward lighting system. An application of the sideward lighting system using diffusive optical fiber belts is described and advantages and disadvantages are discussed.« less

Mechanism of UV-Induced Damage to Mammalian Collagen

DTIC Science & Technology

2014-12-12

enough to use under physiological conditions. We carried out UV photolysis with a UVG – 11 short wavelength hand lamp that emits primarily254 nm. We have...photochemical interaction between UV and mammalian collagen as functions of excitation wavelengths, temperature, fluorescence spectral distribution, and...but has little fluorescence at 325/400 nm (dityrosine). The 325/400 band INCREASES with UV - 1. REPORT DATE (DD-MM-YYYY) 4. TITLE AND SUBTITLE 13

Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light

PubMed Central

Seiler, Franka; Soll, Jürgen; Bölter, Bettina

2017-01-01

Comparative analyses of phenotypic and molecular traits of Arabidopsis thaliana grown under standardised conditions is still a challenge using climatic devices supplied with common light sources. These are in most cases fluorescent lights, which have several disadvantages such as heat production at higher light intensities, an invariable spectral output, and relatively rapid “ageing”. This results in non-desired variations of growth conditions and lowers the comparability of data acquired over extended time periods. In this study, we investigated the growth behaviour of Arabidopsis Col0 under different light conditions, applying fluorescent compared to LED lamps, and we conducted physiological as well as gene expression analyses. By changing the spectral composition and/or light intensity of LEDs we can clearly influence the growth behaviour of Arabidopsis and thereby study phenotypic attributes under very specific light conditions that are stable and reproducible, which is not necessarily given for fluorescent lamps. By using LED lights, we can also roughly mimic the sun light emission spectrum, enabling us to study plant growth in a more natural-like light set-up. We observed distinct growth behaviour under the different light regimes which was reflected by physiological properties of the plants. In conclusion, LEDs provide variable emission spectra for studying plant growth under defined, stable light conditions. PMID:28608805

77 FR 11587 - Certain Dimmable Compact Fluorescent Lamps and Products Containing Same; Institution of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-27

... 361100. Technical Consumer Products, Inc., 325 Campus Drive, Aurora, OH 44202. TCP China, Shanghai Office, 2208-2210 Room, 2nd Building, 270 CaoXi, Road, Xuhui District, Shanghai, China. TCP (Shanghai...

Improved LED backlight with unique color and intensity control and NVIS capability

NASA Astrophysics Data System (ADS)

Herman, Robert; Zagar, Pete; Ulijasz, Ted; Hansen, Hans C.; Ellner, Fred

2006-05-01

Currently deployed conventional flat panel AMLCD displays employ fluorescent lamp backlights to achieve the required lighting levels for cockpits in high performance aircraft. Advances have been made in backlighting technology by replacing fluorescent lamps with high performance LEDs. However, these new LED-based backlights are lacking in control of color and luminance intensity especially when related to NVIS requirements in a cockpit. This paper describes a unique integration of LED, electronic, and optical components to meet the requirements of high performance aircraft over their extreme range of operating environments. The LED-based backlight utilizes state-of-art components to enable daylight, night, and NVIS requirements to be implemented in a simple cost-effective package. The performance results presented highlight the advantages of this new design when compared to currently available backlighting designs. Techniques as described in section 2 of this paper are covered under patent application to the US and International Patent Offices.

On the application of CaF2:Eu and SrF2:Eu phosphors in LED based phototherapy lamp

NASA Astrophysics Data System (ADS)

Belsare, P. D.; Moharil, S. V.; Joshi, C. P.; Omanwar, S. K.

2013-06-01

In the last few years the interest of scientific community has been increased towards solid state lighting based on LEDs because of their superior advantages over the conventional fluorescent lamps. As the GaN based LEDs are easily available efforts of the researchers are now on making the new phosphors which are excitable in the near UV region (360-400nm) for solid state lighting. This paper reports the photoluminescence characteristics of CaF2:Eu and SrF2:Eu phosphor prepared by wet chemical method. The violet emission of these phosphors with near UV excitation can be useful in making a phototherapy lamp based on LEDs for treating various skin diseases like acne vulgaris and hyperbilirubinemia.

The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA.

PubMed

Chen, M H; Kuo, S T; Renault, T; Chang, P H

2014-02-01

A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63°C and 60min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10 folds less sensitive than a SYBR Green PCR. These results indicate that the developed LAMP assay is a simple, rapid, sensitive, specific and reliable technique for the detection of abalone herpesvirus. Copyright © 2013 Elsevier B.V. All rights reserved.

Smart coumarin-tagged imprinted polymers for the rapid detection of tamoxifen.

PubMed

Ray, Judith V; Mirata, Fosca; Pérollier, Celine; Arotcarena, Michel; Bayoudh, Sami; Resmini, Marina

2016-03-01

A signalling molecularly imprinted polymer was synthesised for easy detection of tamoxifen and its metabolites. 6-Vinylcoumarin-4-carboxylic acid (VCC) was synthesised from 4-bromophenol to give a fluorescent monomer, designed to switch off upon binding of tamoxifen. Clomiphene, a chlorinated analogue, was used as the template for the imprinting, and its ability to quench the coumarin fluorescence when used in a 1:1 ratio was demonstrated. Tamoxifen and 4-hydroxytamoxifen were also shown to quench coumarin fluorescence. Imprinted and non-imprinted polymers were synthesised using VCC, methacrylic acid as a backbone monomer and ethylene glycol dimethacrylate as cross-linker, and were ground and sieved to particle sizes ranging between 45 and 25 μm. Rebinding experiments demonstrate that the imprinted polymer shows very strong affinity for both clomiphene and tamoxifen, while the non-imprinted polymer shows negligible rebinding. The fluorescence of the imprinted polymer is quenched by clomiphene, tamoxifen and 4-hydroxytamoxifen. The switch off in fluorescence of the imprinted polymer under these conditions could also be detected under a UV lamp with the naked eye, making this matrix suitable for applications when coupled with a sample preparation system.

76 FR 52892 - Energy Conservation Program: Energy Conservation Standards for Fluorescent Lamp Ballasts

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-24

... between the DOE test data and the data submitted by NEMA; describe the methodological changes DOE is... differences between test data obtained by DOE and test data submitted by NEMA; (3) describe the methodological...

Eternal triangle: the interaction of light source, electrical control gear, and optics

NASA Astrophysics Data System (ADS)

S'heeren, Griet

1998-04-01

In this particular 'affair' the participants are less than human but have individual personalities they bring to their relationship with each other. High pressure metal halide lamps such as BriteArc lamps have the highest luminance and radiance of all continuously operating practical light source. Since these lamps have short arcs and are available in power ratings from about 30W to 30kW they have found applications with various optical systems. Besides the lamps, such systems include an electrical control device and an optical system. To fulfil the user's requirements for a specific application, it is not only important to choose the right lamp, but crucial to achieve a harmonious marriage between the light source, electrical control device and the optics. To run a high pressure discharge lamp an ignitor/ballast system is essential This stabilizes the lamp parameters. The chemical components inside the lamp determine the lamp voltage and the gear determines, via the current, the lamp power. These are directly related in the luminance and color temperature of the emitted light. Therefore lamp performance and effective life are dependent on the ignitor, control gear and lamp combination. Since the lamp emits radiation in all directions, collection of the light from a lamp can be improved by using reflectors to deliver the light into a lens system. Since lamps with short arc gaps approach a point source they appear ideal for optical system applications. The shape of the reflector and the focusing of the lamp determine which part of the light is collected out of the light-arc. In the case of an LCD projector, the final light output also depends on the transmission characteristics of the LCD panels. Their nonlinearity causes the color of the emitted light to be different from the lamp color. All these parameters have to be optimized to obtain the highest performance. This leads to the conclusion that a carefully matched combination of lamp, ignitor/ballast and optics should guarantee the best system performance. This paper sets out to provide some guidelines on attempting to achieve a harmonious relationship between the three partners in this particular eternal triangle.

Validation of a Salmonella loop-mediated isothermal amplification assay in animal food.

PubMed

Domesle, Kelly J; Yang, Qianru; Hammack, Thomas S; Ge, Beilei

2018-01-02

Loop-mediated isothermal amplification (LAMP) has emerged as a promising alternative to PCR for pathogen detection in food testing and clinical diagnostics. This study aimed to validate a Salmonella LAMP method run on both turbidimetry (LAMP I) and fluorescence (LAMP II) platforms in representative animal food commodities. The U.S. Food and Drug Administration (FDA)'s culture-based Bacteriological Analytical Manual (BAM) method was used as the reference method and a real-time quantitative PCR (qPCR) assay was also performed. The method comparison study followed the FDA's microbiological methods validation guidelines, which align well with those from the AOAC International and ISO. Both LAMP assays were 100% specific among 300 strains (247 Salmonella of 185 serovars and 53 non-Salmonella) tested. The detection limits ranged from 1.3 to 28 cells for six Salmonella strains of various serovars. Six commodities consisting of four animal feed items (cattle feed, chicken feed, horse feed, and swine feed) and two pet food items (dry cat food and dry dog food) all yielded satisfactory results. Compared to the BAM method, the relative levels of detection (RLODs) for LAMP I ranged from 0.317 to 1 with a combined value of 0.610, while those for LAMP II ranged from 0.394 to 1.152 with a combined value of 0.783, which all fell within the acceptability limit (2.5) for an unpaired study. This also suggests that LAMP was more sensitive than the BAM method at detecting low-level Salmonella contamination in animal food and results were available 3days sooner. The performance of LAMP on both platforms was comparable to that of qPCR but notably faster, particularly LAMP II. Given the importance of Salmonella in animal food safety, the LAMP assays validated in this study holds great promise as a rapid, reliable, and robust method for routine screening of Salmonella in these commodities. Published by Elsevier B.V.

Advanced Research on the Electrode Area of a Low Pressure Hg-Ar Discharge Lamp

NASA Astrophysics Data System (ADS)

Shi, Jianou

The phenomenon of electrical discharge in low pressure Hg-Ar vapor has been under continuous investigation since it was first discovered. Because much work has been done in the positive column, it is, therefore, that the electrode area of the lamp is the main focus of this thesis. To simulate the interface phenomena on a electrode surface, samples, with optically smooth tungsten-barium interfaces were fired in a high vacuum furnace at different temperatures. Measurements were made using surface characterization techniques. It is found that no Ba_3WO _6 is formed on the surface as previously reported in the powder mixing experiments, and the interface consists mainly of BaWO_4. It was discovered in the early 1950's that vaporization of the barium from the cathode in a fluorescent lamp could be reduced tremendously with the addition of 5% of ZrO _2 to the coating mix. However, the reason for this is poorly understood. A possible explanation has been found, and number of tests have been completed to simulate the formation of BaZO_3 under different lamp operating conditions. The measurements and simulation of barium atom and ion number densities are presented. Barium emitted from the electrode surface has a strong interaction with the local plasma. The number density distributions depend mainly on the discharge conditions. A Monte Carlo computer simulation for the barium ion number density is described and the results from the simulation compared to the experimental results obtained by absorption method. It is clear that the ion distribution and phosphor contamination in the electrode area are two closely related issues. XPS is used to measure the chemical composition on the phosphor surface of the lamp. A discussion of calibration methods and the possible compounds forming on the phosphors is then presented. A number of questions have been raised concerning the safety of the lamp and its affects on health related to radiation generated in the electrode area. Typically, outputs other than the visible light have been brought to the attention of general public. Experimental results are presented which address these issues. Combined with theoretical discussions, this provides useful information for the design and application of the fluorescent lamp.

Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting.

PubMed

Wilke, Sonja; Krausze, Joern; Gossen, Manfred; Groebe, Lothar; Jäger, Volker; Gherardi, Ermanno; van den Heuvel, Joop; Büssow, Konrad

2010-06-01

Stable mammalian cell lines are excellent tools for the expression of secreted and membrane glycoproteins. However, structural analysis of these molecules is generally hampered by the complexity of N-linked carbohydrate side chains. Cell lines with mutations are available that result in shorter and more homogenous carbohydrate chains. Here, we use preparative fluorescence-activated cell sorting (FACS) and site-specific gene excision to establish high-yield glycoprotein expression for structural studies with stable clones derived from the well-established Lec3.2.8.1 glycosylation mutant of the Chinese hamster ovary (CHO) cell line. We exemplify the strategy by describing novel clones expressing single-chain hepatocyte growth factor/scatter factor (HGF/SF, a secreted glycoprotein) and a domain of lysosome-associated membrane protein 3 (LAMP3d). In both cases, stable GFP-expressing cell lines were established by transfection with a genetic construct including a GFP marker and two rounds of cell sorting after 1 and 2 weeks. The GFP marker was subsequently removed by heterologous expression of Flp recombinase. Production of HGF/SF and LAMP3d was stable over several months. 1.2 mg HGF/SF and 0.9 mg LAMP3d were purified per litre of culture, respectively. Homogenous glycoprotein preparations were amenable to enzymatic deglycosylation under native conditions. Purified and deglycosylated LAMP3d protein was readily crystallized. The combination of FACS and gene excision described here constitutes a robust and fast procedure for maximizing the yield of glycoproteins for structural analysis from glycosylation mutant cell lines.

Hyperfine structure investigations for the odd-parity configuration system in atomic holmium

NASA Astrophysics Data System (ADS)

Stefanska, D.; Furmann, B.

2018-02-01

In this work new experimental results of the hyperfine structure (hfs) in the holmium atom are reported, concerning the odd-parity level system. Investigations were performed by the method of laser induced fluorescence in a hollow cathode discharge lamp on 97 spectral lines in the visible part of the spectrum. Hyperfine structure constants: magnetic dipole - A and electric quadrupole - B for 40 levels were determined for the first time; for another 21 levels the hfs constants available in the literature were remeasured. Results for the A constants can be viewed as fully reliable; for B constants further possibilities of improving the accuracy are considered.

New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination

PubMed Central

Karthik, K.; Rathore, Rajesh; Thomas, Prasad; Arun, T.R.; Viswas, K.N.; Dhama, Kuldeep; Agarwal, R.K.

2014-01-01

Loop mediated isothermal amplification (LAMP) assay, a promising diagnostic test, has been developed for detection of different pathogens of human as well as animals. Various positive points support its use as a field level test but the major problem is product cross contamination leading to false positive results. Different methods were adopted by various researchers to control this false positive amplification due to cross contamination but all have their own advantages and disadvantages. A new closed tube LAMP assay based on agar dye capsule was developed in the present study and this technique has some advantages over the other closed tube technique.•Agar at the concentration of 1.5% was used to sandwich SYBR green dye I with the aid of intradermal syringe. This agar dye capsule was placed over the LAMP reaction mixture before it was amplified.•To eliminate the hazardous nature of Ultra Violet (UV) light during result visualization of LAMP products, the present study demonstrates the use of Light Emitting Diode (LED) lights for result visualization.•LAMP was carried out for Brucella species detection using this modified techniques yielding good results without any cross contamination and LED showed similar fluorescence compared to UV. PMID:26150945

New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination.

PubMed

Karthik, K; Rathore, Rajesh; Thomas, Prasad; Arun, T R; Viswas, K N; Dhama, Kuldeep; Agarwal, R K

2014-01-01

Loop mediated isothermal amplification (LAMP) assay, a promising diagnostic test, has been developed for detection of different pathogens of human as well as animals. Various positive points support its use as a field level test but the major problem is product cross contamination leading to false positive results. Different methods were adopted by various researchers to control this false positive amplification due to cross contamination but all have their own advantages and disadvantages. A new closed tube LAMP assay based on agar dye capsule was developed in the present study and this technique has some advantages over the other closed tube technique.•Agar at the concentration of 1.5% was used to sandwich SYBR green dye I with the aid of intradermal syringe. This agar dye capsule was placed over the LAMP reaction mixture before it was amplified.•To eliminate the hazardous nature of Ultra Violet (UV) light during result visualization of LAMP products, the present study demonstrates the use of Light Emitting Diode (LED) lights for result visualization.•LAMP was carried out for Brucella species detection using this modified techniques yielding good results without any cross contamination and LED showed similar fluorescence compared to UV.

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii.

PubMed

Chen, Hua-Wei; Ching, Wei-Mei

2016-04-18

Coxiella burnetii, the agent causing Q fever, is an obligate intracellular bacterium. PCR based diagnostic assays have been developed for detecting C. burnetii DNA in cell cultures and clinical samples. PCR requires specialized equipment and extensive end user training, and therefore, it is not suitable for routine work especially in a resource-constrained area. We have developed a loop-mediated isothermal amplification (LAMP) assay to detect the presence of C. burnetii in patient samples. This method is performed at a single temperature around 60 °C in a water bath or heating block. The sensitivity of this LAMP assay is very similar to PCR with a detection limit of about 25 copies per reaction. This report describes the preparation of the reaction using lyophilized reagents and visualization of results using hydroxynaphthol blue (HNB) or a UV lamp with fluorescent intercalating dye in the reaction. The LAMP reagents were lyophilized and stored at room temperature (RT) for one month without loss of detection sensitivity. This LAMP assay is particularly robust because the reaction mixture preparation does not involve complex steps. This method is ideal for use in resource-limited settings where Q fever is endemic.

A new fluorescent enhanced probe based on (E)-9-(2-nitrovinyl)-anthracene for the detection of bisulfite anions and its practical application

NASA Astrophysics Data System (ADS)

Chao, Jianbin; Liu, Yuhong; Zhang, Yan; Zhang, Yongbin; Huo, Fangjun; Yin, Caixia; Wang, Yu; Qin, Liping

2015-07-01

A new fluorescent enhanced probe based on (E)-9-(2-nitrovinyl)-anthracene is developed, which shows high selectivity and sensitivity for the detection of bisulfite anions at Na2HPO4 citric acid buffer solutions (pH 5.0). When addition of HSO3-, the fluorescence intensity is significantly enhanced and the probe displays apparent fluorescence color changes from non-fluorescence to blue under a UV lamp illumination, the solution color also changes from yellow to colorless. The detection limit is determined to be as low as 6.30 μM. This offers another specific colorimetric and fluorescent probe for bisulfite anions detection, furthermore it is applied in detecting the level of bisulfite in sugar samples.

PHOTOCHEMICAL REACTIONS AMONG FORMALDEHYDE, CHLORINE, AND NITROGEN DIOXIDE IN AIR

EPA Science Inventory

Photochemical reactions among chlorine, nitrogen dioxide, and formaldehyde were studied, using parts-per-million concentrations in 1 atm of air. The reactant mixtures were irradiated by ultraviolet fluorescent lamps and simultaneously analyzed by the Fourier transform infrared te...

Is It Time for a Switch in Lighting?

ERIC Educational Resources Information Center

Bennorth, Greg

1999-01-01

Reviews new types of fluorescent lamp and electronic ballasts and their associated starting methods to explain the benefits of programmed start ballasts in today's lighting applications. Advice on when it is time to make a lighting retrofit is highlighted. (GR)

78 FR 24233 - Certain Dimmable Compact Fluorescent Lamps and Products Containing Same: Notice of Institution of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-24

... information concerning the Commission may also be obtained by accessing its Internet server at http://www... complaint alleged, among other things, that the importation into the United States, the sale for importation...

Cases of acute mercury poisoning by mercury vapor exposure during the demolition of a fluorescent lamp factory.

PubMed

Do, Sang Yoon; Lee, Chul Gab; Kim, Jae Yoon; Moon, Young Hoon; Kim, Min Sung; Bae, In Ho; Song, Han Soo

2017-01-01

In 2015, workers dismantling a fluorescent lamp factory in Korea were affected by mercury poisoning from exposure to mercury vapor. Eighteen out of the 21 workers who participated in the demolition project presented with symptoms of poisoning and, of these, 10 had persistent symptoms even at 18 months after the initial exposure to mercury vapor. Early symptoms of 18 workers included a general skin rash, pruritus, myalgia, sleep disturbance, and cough and sputum production. Following alleviation of these initial symptoms, late symptoms, such as easy fatigue, insomnia, bad dreams, and anxiety disorder, began to manifest in 10 out of 18 patients. Seven workers underwent psychiatric care owing to sleep disturbance, anxiety disorder, and depression, and three workers underwent dermatologic treatment for hyperpigmentation, erythematous skin eruption, and chloracne-like skin lesions. Furthermore, three workers developed a coarse jerky movement, two had swan neck deformity of the fingers, and two received care at an anesthesiology clinic for paresthesia, such as burning sensation, cold sensation, and pain. Two workers underwent urologic treatment for dysfunction of the urologic system and impotence. However, symptomatic treatment did not result in satisfactory relief of these symptoms. Awareness of the perils of mercury and prevention of mercury exposure are critical for preventing health hazards caused by mercury vapor. Chelation therapy should be performed promptly following mercury poisoning to minimize damage.

Internode and petiole elongation of soybean in response to photoperiod and end-of-day light quality

NASA Technical Reports Server (NTRS)

Thomas, J. F.; Raper, C. D. Jr; Raper CD, J. r. (Principal Investigator)

1985-01-01

Elongation of main stem internodes and petioles of soybeans, Glycine max 'Ransom,' was examined in response to various photoperiod/temperature combinations and to end-of-day (EOD) light quality. Photoperiod treatments consisted of 10, 14, and 16 h in combination with day/night temperatures of 18/14, 22/18, 26/22, 30/26, and 34/30 C. The EOD treatments consisted of exposing plants to illumination from either incandescent (high far-red component, FR) or fluorescent (high red component, R) lamps during the final 0.5 h of a 10-h photoperiod. Internode elongation was not significantly promoted by the photoperiod treatments, and, in fact, under the two highest temperature regimes, internode elongation was suppressed under the longer photoperiods. Petiole elongation, however, was enhanced under the longer photoperiods at all temperatures. In the EOD light study, internode and petiole elongation was significantly greater on plants exposed to 0.5 h EOD from incandescent lamps than from fluorescent. Under the incandescent EOD treatment, plants increased dry matter production by 41% and exhibited greater partitioning of assimilates in stem and root portions than under fluorescent EOD.

A norovirus detection architecture based on isothermal amplification and expanded genetic systems.

PubMed

Yaren, Ozlem; Bradley, Kevin M; Moussatche, Patricia; Hoshika, Shuichi; Yang, Zunyi; Zhu, Shu; Karst, Stephanie M; Benner, Steven A

2016-11-01

Noroviruses are the major cause of global viral gastroenteritis with short incubation times and small inoculums required for infection. This creates a need for a rapid molecular test for norovirus for early diagnosis, in the hope of preventing the spread of the disease. Non-chemists generally use off-the shelf reagents and natural DNA to create such tests, suffering from background noise that comes from adventitious DNA and RNA (collectively xNA) that is abundant in real biological samples, especially feces, a common location for norovirus. Here, we create an assay that combines artificially expanded genetic information systems (AEGIS, which adds nucleotides to the four in standard xNA, pairing orthogonally to A:T and G:C) with loop-mediated isothermal amplification (LAMP) to amplify norovirus RNA at constant temperatures, without the power or instrument requirements of PCR cycling. This assay was then validated using feces contaminated with murine norovirus (MNV). Treating stool samples with ammonia extracts the MNV RNA, which is then amplified in an AEGIS-RT-LAMP where AEGIS segments are incorporated both into an internal LAMP primer and into a molecular beacon stem, the second lowering background signaling noise. This is coupled with RNase H nicking during sample amplification, allowing detection of as few as 10 copies of noroviral RNA in a stool sample, generating a fluorescent signal visible to human eye, all in a closed reaction vessel. Copyright © 2016 Elsevier B.V. All rights reserved.

Laser induced photoluminiscence studies of primary photochemical production processes of cometary radicals

NASA Technical Reports Server (NTRS)

Jackson, W. M.

1977-01-01

A tunable vacuum ultraviolet flash lamp was constructed. This unique flash lamp was coupled with a tunable dye laser detector and permits the experimenter to measure the production rates of ground state radicals as a function of wavelength. A new technique for producing fluorescent radicals was discovered. This technique called multiphoton ultraviolet photodissociation is currently being applied to several problems of both cometary and stratospheric interest. It was demonstrated that NO2 will dissociate to produce an excited fragment and the radiation can possibly be used for remote detection of this species.

[Tanning lamp radiation-induced photochemical retinal damage].

PubMed

Volkov, V V; Kharitonova, N N; Mal'tsev, D S

2014-01-01

On the basis of original clinical research a rare case of bilateral retinal damage due to tanning lamp radiation exposure is presented. Along with significant decrease of visual acuity and light sensitivity of central visual field as well as color vision impairment, bilateral macular dystrophy was found during an ophthalmoscopy and confirmed by optical coherent tomography and fluorescent angiography. Intensive retinoprotective, vascular, and antioxidant therapy was effective and led to functional improvement and stabilization of the pathologic process associated with photochemical retinal damage. A brief review of literature compares mechanisms of retinal damage by either short or long-wave near visible radiation.

Replacement policy of residential lighting optimized for cost, energy, and greenhouse gas emissions

NASA Astrophysics Data System (ADS)

Liu, Lixi; Keoleian, Gregory A.; Saitou, Kazuhiro

2017-11-01

Accounting for 10% of the electricity consumption in the US, artificial lighting represents one of the easiest ways to cut household energy bills and greenhouse gas (GHG) emissions by upgrading to energy-efficient technologies such as compact fluorescent lamps (CFL) and light emitting diodes (LED). However, given the high initial cost and rapidly improving trajectory of solid-state lighting today, estimating the right time to switch over to LEDs from a cost, primary energy, and GHG emissions perspective is not a straightforward problem. This is an optimal replacement problem that depends on many determinants, including how often the lamp is used, the state of the initial lamp, and the trajectories of lighting technology and of electricity generation. In this paper, multiple replacement scenarios of a 60 watt-equivalent A19 lamp are analyzed and for each scenario, a few replacement policies are recommended. For example, at an average use of 3 hr day-1 (US average), it may be optimal both economically and energetically to delay the adoption of LEDs until 2020 with the use of CFLs, whereas purchasing LEDs today may be optimal in terms of GHG emissions. In contrast, incandescent and halogen lamps should be replaced immediately. Based on expected LED improvement, upgrading LED lamps before the end of their rated lifetime may provide cost and environmental savings over time by taking advantage of the higher energy efficiency of newer models.

CALiPER Exploratory Study. Recessed Troffer Lighting

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miller, N. J.; Royer, M. P.; Poplawski, M. E.

This CALiPER study examines the problems and benefits likely to be encountered with LED products intended to replace linear fluorescent lamps. LED dedicated troffers, replacement tubes, and non-tube retrofit kits were evaluated against fluorescent benchmark troffers in a simulated office space for photometric distribution, uniformity of light on the task surface, suitability of light output, flicker, dimming performance, color quality, power quality, safety and certification issues, ease of installation, energy efficiency, and life-cycle cost.

[Remote Slit Lamp Microscope Consultation System Based on Web].

PubMed

Chen, Junfa; Zhuo, Yong; Liu, Zuguo; Chen, Yanping

2015-11-01

To realize the remote operation of the slit lamp microscope for department of ophthalmology consultation, and visual display the real-time status of remote slit lamp microscope, a remote slit lamp microscope consultation system based on B/S structure is designed and implemented. Through framing the slit lamp microscope on the website system, the realtime acquisition and transmission of remote control and image data is realized. The three dimensional model of the slit lamp microscope is established and rendered on the web by using WebGL technology. The practical application results can well show the real-time interactive of the remote consultation system.

Bright Ideas.

ERIC Educational Resources Information Center

Armstrong, Phil

1999-01-01

Discusses how to upgrade lighting technology in schools to reduce energy consumption and cut operating costs. Explores fixture efficiency using ballast and lamp upgrades and compact fluorescent lights. Other ideas include changing exit signs to ones that use less wattage, improving luminary efficiency through use of reflectors and shielding…

A field-deployable mobile molecular diagnostic system for malaria at the point of need.

PubMed

Choi, Gihoon; Song, Daniel; Shrestha, Sony; Miao, Jun; Cui, Liwang; Guan, Weihua

2016-11-01

In response to the urgent need of a field-deployable and highly sensitive malaria diagnosis, we developed a standalone, "sample-in-answer-out" molecular diagnostic system (AnyMDx) to enable quantitative molecular analysis of blood-borne malaria in low resource areas. The system consists of a durable battery-powered analyzer and a disposable microfluidic compact disc loaded with reagents ready for use. A low power thermal module and a novel fluorescence-sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP) of target parasite DNA. With 10 μL of raw blood sample, the AnyMDx system automates the nucleic acid sample preparation and subsequent LAMP and real-time detection. Under laboratory conditions with whole-blood samples spiked with cultured Plasmodium falciparum, we achieved a detection limit of ∼0.6 parasite per μL, much lower than those for the conventional microscopy and rapid diagnostic tests (∼50-100 parasites per μL). The turnaround time from sample to answer is less than 40 minutes. The AnyMDx is user-friendly requiring minimal technological training. The analyzer and the disposable reagent compact discs are cost-effective, making AnyMDx a potential tool for malaria molecular diagnosis under field settings for malaria elimination.

Optimizing environmental conditions for mass application of mechano-dwarfing stimuli to Arabidopsis

NASA Technical Reports Server (NTRS)

Montgomery, Jill A.; Bressan, Ray A.; Mitchell, Cary A.

2004-01-01

Obtaining uniform mechano-dwarfing of Arabidopsis thaliana (L.) Heynh. seedlings within dense plantings is problematic. Alternative forms of mechano-stimulation were applied to seedlings in effort to obtain uniform growth reduction compared with undisturbed controls in both greenhouse and controlled growth environments. Arabidopsis grown under low photosynthetic photon flux (PPF) artificial light grew upright with limited leaf expansion, which enhanced mechano-responsiveness compared to that of rosette-growing plants under filtered sunlight or high PPF artificial light. Hypocotyls of seedlings grown at PPFs > 60 micromoles m-2 s-1 elongated less and had 6% less sensitivity to mechanical stress than seedlings grown at PPFs < 60 micromoles m-2 s-1. Fluorescent lamps alone (F) or fluorescent plus incandescent (F+I) lamps were compared for seedling responses to mechanical stress. Under F lighting, hypocotyl elongation was reduced 25% to 40% by twice-daily brush or plate treatments, and brushed seedlings exhibited more growth reduction than did plate treatments. Seedlings grown under F+I lamps exhibited similar stress-induced growth reduction compared to seedlings grown under F only, but stressed F+I seedlings lodged to a greater extent due to excessive hypocotyl elongation. Temperature-response studies using standardized F-only lighting indicated increased hypocotyl elongation but decreased leaf expansion, and decreased mechano-responsivity to brushing over the temperature range from 20 to 28 degrees C. Daylength studies indicated similar degrees of mechano-inhibition of hypocotyl elongation over the daylength range of 12, 16, 20, and 24 hours, whereas fresh weight of stressed seedling shoots declined compared to controls. A combination of environmental growth parameters that give repeatable, visual mechanical dwarfing of Arabidopsis include low-PPF fluorescent lighting from 55 to 60 micromoles m-2 s-1, ambient temperatures from 22 to 25 degrees C, and twice-daily brush treatments.

Rapid and Sensitive Detection of Vibrio parahaemolyticus and Vibrio vulnificus by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique.

PubMed

Wang, Yi; Li, Dongxun; Wang, Yan; Li, Kewei; Ye, Changyun

2016-01-19

Vibrio parahaemolyticus and Vibrio vulnificus are two marine seafood-borne pathogens causing severe illnesses in humans and aquatic animals. In this study, a recently developed novel multiple endonuclease restriction real-time loop-mediated isothermal amplification technology (MERT-LAMP) were successfully developed and evaluated for simultaneous detection of V. parahaemolyticus and V. vulnificus strains in only a single reaction. Two MERT-LAMP primer sets were designed to specifically target toxR gene of V. parahaemolyticus and rpoS gene of V. vulnificus. The MERT-LAMP reactions were conducted at 62 °C, and the positive results were produced in as short as 19 min with the genomic DNA templates extracted from the V. parahaemolyticus and V. vulnificus strains. The two target pathogens present in the same sample could be simultaneously detected and correctly differentiated based on distinct fluorescence curves in a real-time format. The sensitivity of MERT-LAMP assay was 250 fg and 125 fg DNA per reaction with genomic templates of V. parahaemolyticus and V. vulnificus strains, which was in conformity with conventional LAMP detection. Compared with PCR-based techniques, the MERT-LAMP technology was 100- and 10-fold more sensitive than that of PCR and qPCR methods. Moreover, the limit of detection of MERT-LAMP approach for V. parahaemolyticus isolates and V. vulnificus isolates detection in artificially-contaminated oyster samples was 92 CFU and 83 CFU per reaction. In conclusion, the MERT-LAMP assay presented here was a rapid, specific, and sensitive tool for the detection of V. parahaemolyticus and V. vulnificus, and could be adopted for simultaneous screening of V. parahaemolyticus and V. vulnificus in a wide variety of samples.

Gelcasting polycrystalline alumina

DOE Office of Scientific and Technical Information (OSTI.GOV)

Janney, M.A.

1997-04-01

This work is being done as part of a CRADA with Osram-Sylvania, Inc. (OSI) OSI is a major U.S. manufacturer of high-intensity lighting. Among its products is the Lumalux{reg_sign} line of high-pressure sodium vapor arc lamps, which are used for industrial, highway, and street lighting. The key to the performance of these lamps is the polycrystalline alumina (PCA) tube that is used to contain the plasma that is formed in the electric arc. That plasma consists of ionized sodium, mercury, and xenon vapors. The key attributes of the PCA tubes are their transparency (95% total transmittance in the visible region),more » their refractoriness (inner wall temperature can reach 1400{degrees}C), and their chemical resistance (sodium and mercury vapor are extremely corrosive). The current efficiency of the lamps is very high, on the order of several hundred lumens / watt. (Compare - incandescent lamps -13 lumens/watt fluorescent lamps -30 lumens/watt.) Osram-Sylvania would like to explore using gelcasting to form PCA tubes for Lumalux{reg_sign} lamps, and eventually for metal halide lamps (known as quartz-halogen lamps). Osram-Sylvania, Inc. currently manufactures PCA tubes by isostatic pressing. This process works well for the shapes that they presently use. However, there are several types of tubes that are either difficult or impossible to make by isostatic pressing. It is the desire to make these new shapes and sizes of tubes that has prompted Osram-Sylvania`s interest in gelcasting. The purpose of the CRADA is to determine the feasibility of making PCA items having sufficient optical quality that they are useful in lighting applications using gelcasting.« less

Fluorescence spectroscopy of soil pellets : The use of CP/PARAFAC.

NASA Astrophysics Data System (ADS)

Mounier, Stéphane; Nicolodeli, Gustavo; Redon, Roland; Hacherouf, Kalhed; Milori, Debora M. B. P.

2014-05-01

Fluorescence spectroscopy is one of the most sensitive techniques available for analytical purposes. It is relatively easy to implement, phenomenologically straightforward and well investigated. Largely non-invasive and fast, so that it can be useful for environmental applications. Fluorescence phenomenon is highly probable in molecular systems containing atoms with lone pairs of electrons such as C=O, aromatic, phenolic, quinone and more rigid unsaturated conjugated systems. These functional groups are present in humic substances (HS) from soils (Senesi, 1990; N. Senesi et al., 1991) and represent the main fluorophors of Soil Organic Matter (SOM). The extension of the conjugated electronic system, the level of heteroatom substitution and type and number of substituting groups under the aromatic rings strongly affect the intensity and wavelength of molecular fluorescence. However, to analyse the SOM it is generally done a chemical extraction that allows measuring the fluorescence response of the liquid extract. To avoid this fractionation of the SOM, Milori et al. (2006) proposed the application of laser induced fluorescence spectroscopy (LIFS) in whole soil. This work intends to assess the technical feasibility of 3D fluorescence spectroscopy using lamp for excitation to analyse solids opaque samples prepared with different substances. Seventy four (74) solid samples were prepared from different mixtures of boric acid (BA), humic substance acid and tryptophan (TRP) powder. The compounds were mixture and a pellet was done by using pressure (8 ton). The pellets were measured using a spectrofluorimeter HITACHI F4500, and a 3D fluorescence tensor was done from emission spectra (200-600 nm) with excitation range from 200 to 500 nm. The acquisition parameters were: step at 5 nm, scan speed at 2400 nm.min-1, response time at 0.1 s, excitation and emission slits at 5 nm and photomultiplier voltage at 700 V. Furthermore, measures of Laser-induced Fluorescence were performed in pellets (boric and humic acids mixture) using a portable system built by Embrapa Instrumentation. It comprises a diode laser (Coherent - CUBE) emitting at 405 nm (50 mW), and the detection of emission by a high sensitivity mini-spectrometer (USB4000 - Ocean Optics) using a range from 440 to 800 nm. In first step, the 3D tensors were then treated by the CP/PARAFAC algorithm to decompose the signal response after removing the diffusion signal : three components were extracted with a CORCONDIA over 60%. The first component can be associate an artefact of the measurement or boric acid fluorescence, the second and third component could the related to the two different fluorescence contributions of tryptophan molecule, one with central excitation/emission in 290/360 nm and other in 350/465 nm. The presence of a small quantity (i.e. few percent in mass) of humic acid (HA) is quenching drastically the TRP fluorescence. Complementary, measurements will be performed to understand this behaviour taking in account the absorption wavelength by the surface (colour) and by measuring the time life fluorescence of the samples. Humic acid fluorescence in pellets (BA and HA) cannot be observed using lamp + monochromator excitation due to low intensity of source. The same pellets were measure using LIFS system, and fluorescence intensity increased as a function of concentration of HA until occur the inner filter effect from 300 ppm, similar to the behaviour of HA in solution. Even whether solid surface measurements are easier, understanding is not yet clear. More investigation needs to be done. Moreover, it should be important to know if the use of CP/PARAFAC decomposition for such data is relevant with the trilinear model. References Milori, D.M.B.P., Galeti, H.V.A., Martin-Neto, L., Dieckow, J., González-Pérez, M., Bayer, C., Salton, J., 2006. Organic Matter Study of Whole Soil Samples Using Laser-Induced Fluorescence Spectroscopy. Soil Science Society of America Journal 70, 57. N. Senesi, TM, M., MR, P., Brunetti., G., 1991. Characterization, differentiation and classification of humic substances by fluorescence spectroscopy. Soil Science 152, 259-271. Senesi, N., 1990. Molecular and quantitative aspects of the chemistry of fulvic acid and its interactions with metal ions and organic chemicals. Analytica Chimica Acta 232, 77-106.

16 CFR 305.4 - Prohibited acts.

Code of Federal Regulations, 2014 CFR

2014-01-01

... advertisement, with respect to the energy use or efficiency or, in the case of showerheads, faucets, water... showerheads, faucets, water closets and urinals, water use of such product, or cost of energy consumed by such... conditioners, pulse combustion and condensing furnaces, fluorescent lamp ballasts, showerheads, faucets, water...

Comparative Study of Lettuce and Radish Grown Under Red and Blue Light-Emitting Diodes (LEDs) and White Fluorescent Lamps

NASA Technical Reports Server (NTRS)

Mickens, Matthew A.

2012-01-01

Growing vegetable crops in space will be an essential part of sustaining astronauts during long-term missions. To drive photosynthesis, red and blue light-emitting diodes (LEDs) have attracted attention because of their efficiency, longevity, small size, and safety. In efforts to optimize crop production, there have also been recent interests in analyzing the subtle effects of green light on plant growth, and to determine if it serves as a source of growth enhancement or suppression. A comparative study was performed on two short cycle crops of lettuce (Outredgeous) and radish (Cherry Bomb) grown under two light treatments. The first treatment being red and blue LEDs, and the second treatment consisting of white fluorescent lamps which contain a portion of green light. In addition to comparing biomass production, physiological characterizations were conducted on how the light treatments influence morphology, water use, chlorophyll content, and the production of A TP within plant tissues.

Human exposure to mercury in a compact fluorescent lamp manufacturing area: By food (rice and fish) consumption and occupational exposure.

PubMed

Liang, Peng; Feng, Xinbin; Zhang, Chan; Zhang, Jin; Cao, Yucheng; You, Qiongzhi; Leung, Anna Oi Wah; Wong, Ming-Hung; Wu, Sheng-Chun

2015-03-01

To investigate human Hg exposure by food consumption and occupation exposure in a compact fluorescent lamp (CFL) manufacturing area, human hair and rice samples were collected from Gaohong town, Zhejiang Province, China. The mean values of total mercury (THg) and methylmercury (MeHg) concentrations in local cultivated rice samples were significantly higher than in commercial rice samples which indicated that CFL manufacturing activities resulted in Hg accumulation in local rice samples. For all of the study participants, significantly higher THg concentrations in human hair were observed in CFL workers compared with other residents. In comparison, MeHg concentrations in human hair of residents whose diet consisted of local cultivated rice were significantly higher than those who consumed commercial rice. These results demonstrated that CFL manufacturing activities resulted in THg accumulation in the hair of CFL workers. However, MeHg in hair were mainly affected by the sources of rice of the residents. Copyright © 2014 Elsevier Ltd. All rights reserved.

Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a highly sensitive detection of enterovirus in the stool samples of acute flaccid paralysis cases.

PubMed

Arita, Minetaro; Ling, Hua; Yan, Dongmei; Nishimura, Yorihiro; Yoshida, Hiromu; Wakita, Takaji; Shimizu, Hiroyuki

2009-12-16

In the global eradication program for poliomyelitis, the laboratory diagnosis plays a critical role by isolating poliovirus (PV) from the stool samples of acute flaccid paralysis (AFP) cases. In this study, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a rapid and highly sensitive detection of enterovirus including PV to identify stool samples positive for enterovirus including PV. A primer set was designed for RT-LAMP to detect enterovirus preferably those with PV-like 5'NTRs of the viral genome. The sensitivity of RT-LAMP system was evaluated with prototype strains of enterovirus. Detection of enterovirus from stool extracts was examined by using RT-LAMP system. We detected at least 400 copies of the viral genomes of PV(Sabin) strains within 90 min by RT-LAMP with the primer set. This RT-LAMP system showed a preference for Human enterovirus species C (HEV-C) strains including PV, but exhibited less sensitivity to the prototype strains of HEV-A and HEV-B (detection limits of 7,400 to 28,000 copies). Stool extracts, from which PV, HEV-C, or HEV-A was isolated in the cell culture system, were mostly positive by RT-LAMP method (positive rates of 15/16 (= 94%), 13/14 (= 93%), and 4/4 (= 100%), respectively). The positive rate of this RT-LAMP system for stool extracts from which HEV-B was isolated was lower than that of HEV-C (positive rate of 11/21 (= 52%)). In the stool samples, which were negative for enterovirus isolation by the cell culture system, we found that two samples were positive for RT-LAMP (positive rates of 2/38 (= 5.3%)). In these samples, enterovirus 96 was identified by sequence analysis utilizing a seminested PCR system. RT-LAMP system developed in this study showed a high sensitivity comparable to that of the cell culture system for the detection of PV, HEV-A, and HEV-C, but less sensitivity to HEV-B. This RT-LAMP system would be useful for the direct detection of enterovirus from the stool extracts.

Spectral quality may be used to alter plant disease development in CELSS

NASA Astrophysics Data System (ADS)

Schuerger, A. C.; Brown, C. S.

1994-11-01

Plants were grown under light emitting diode (LED) arrays with different spectral qualities to determine the effects of light on the development of tomato mosaic virus (ToMV) in peppers and powdery mildew on cucumbers. One LED array supplied 100% of the photosynthetic photon flux (PPF) at 660 nm, a second array supplied 90% of the PPF at 660 nm and 10% at 735 nm, and a third array supplied 98% of the PPF at 660 nm with 2% in the blue region (380-500 nm) supplied by blue fluorescent lamps. Control plants were grown under metal halide (MH) lamps. Pepper plants inoculated with ToMV and grown under 660 and 660/735 LED arrays showed marked increases in both the rate and the severity of symptoms as compared to inoculated plants grown under the MH lamp or 660/blue array. Pepper plants grown under the 660/blue array did not develop symptoms as rapidly as inoculated plants grown under the 660 or 660/735 arrays, but they did develop symptoms faster than inoculated plants grown under the MH lamp. The numbers of colonies of powdery mildew per leaf and the size of each colony were greatest on inoculated cucumber plants grown under the MH lamp.

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection.

PubMed

Nzelu, Chukwunonso O; Gomez, Eduardo A; Cáceres, Abraham G; Sakurai, Tatsuya; Martini-Robles, Luiggi; Uezato, Hiroshi; Mimori, Tatsuyuki; Katakura, Ken; Hashiguchi, Yoshihisa; Kato, Hirotomo

2014-04-01

Entomological monitoring of Leishmania infection in leishmaniasis endemic areas offers epidemiologic advantages for predicting the risk and expansion of the disease, as well as evaluation of the effectiveness of control programs. In this study, we developed a highly sensitive loop-mediated isothermal amplification (LAMP) method for the mass screening of sand flies for Leishmania infection based on the 18S rRNA gene. The LAMP technique could detect 0.01 parasites, which was more sensitive than classical PCR. The method was robust and could amplify the target DNA within 1h from a crude sand fly template without DNA purification. Amplicon detection could be accomplished by the newly developed colorimetric malachite green (MG)--mediated naked eye visualization. Pre-addition of MG to the LAMP reaction solution did not inhibit amplification efficiency. The field applicability of the colorimetric MG-based LAMP assay was demonstrated with 397 field-caught samples from the endemic areas of Ecuador and eight positive sand flies were detected. The robustness, superior sensitivity, and ability to produce better visual discriminatory reaction products than existing LAMP fluorescence and turbidity assays indicated the field potential usefulness of this new method for surveillance and epidemiological studies of leishmaniasis in developing countries. Copyright © 2013 Elsevier B.V. All rights reserved.

Characterization of aeroallergen of Texas panhandle using scanning and fluorescence microscopy

NASA Astrophysics Data System (ADS)

Ghosh, Nabarun; Whiteside, Mandy; Ridner, Chris; Celik, Yasemin; Saadeh, C.; Bennert, Jeff

2010-06-01

Aeroallergens cause serious allergic and asthmatic reactions. Characterizing the aeroallergen provides information regarding the onset, duration, and severity of the pollen season that clinicians use to guide allergen selection for skin testing and treatment. Fluorescence Microscopy has useful approaches to understand the structure and function of the microscopic objects. Prepared slides from the pollen were observed under an Olympus BX40 microscope equipped with FITC and TRITC fluorescent filters, a mercury lamp source, an Olympus DP-70 digital camera connected to the computer with Image Pro 6.0 software. Aeroallergens were viewed, recorded and analyzed with DP Manager using the Image Pro 6.0 software. Photographs were taken at bright field, the fluorescein-isothiocyanate (FITC) filter, and the tetramethylrhodamine (TRITC) filter settings at 40X. A high pressure mercury lamp or UV source was used to excite the storage molecules or proteins which exhibited autofluorescence. The FITC filter reveals the green fluorescent proteins (GFP and EGFP), and the TRITC filter for red fluorescent proteins (DsRed). SEM proved to be useful for observing ultra-structural details like pores, colpi, sulci and ornamentations on the pollen surface. Samples were examined with an SEM (TM-1000) after gold coating and Critical Point Drying. Pollen grains were measured using the TM-1000 imaging software that revealed the specific information on the size of colpi or sulci and the distance between the micro-structures. This information can be used for classification and circumscription in Angiosperm taxonomy. Data were correlated clinical studies established at Allergy A.R.T.S. Clinical Research Laboratory.

Terbium(III)/gold nanocluster conjugates: the development of a novel ratiometric fluorescent probe for mercury(II) and a paper-based visual sensor.

PubMed

Qi, Yan-Xia; Zhang, Min; Zhu, Anwei; Shi, Guoyue

2015-08-21

In this work, a novel ratiometric fluorescent probe was developed for rapid, highly accurate, sensitive and selective detection of mercury(II) (Hg(2+)) based on terbium(III)/gold nanocluster conjugates (Tb(3+)/BSA-AuNCs), in which bovine serum albumin capped gold nanoclusters (BSA-AuNCs) acted as the signal indicator and terbium(III) (Tb(3+)) was used as the build-in reference. Our proposed ratiometric fluorescent probe exhibited unique specificity toward Hg(2+) against other common environmentally and biologically important metal ions, and had high accuracy and sensitivity with a low detection limit of 1 nM. In addition, our proposed probe was effectively employed to detect Hg(2+) in the biological samples from the artificial Hg(2+)-infected rats. More significantly, an appealing paper-based visual sensor for Hg(2+) was designed by using filter paper embedded with Tb(3+)/BSA-AuNC conjugates, and we have further demonstrated its feasibility for facile fluorescent sensing of Hg(2+) in a visual format, in which only a handheld UV lamp is used. In the presence of Hg(2+), the paper-based visual sensor, illuminated by a handheld UV lamp, would undergo a distinct fluorescence color change from red to green, which can be readily observed with naked eyes even in trace Hg(2+) concentrations. The Tb(3+)/BSA-AuNC-derived paper-based visual sensor is cost-effective, portable, disposable and easy-to-use. This work unveiled a facile approach for accurate, sensitive and selective measuring of Hg(2+) with self-calibration.

Rapid detection of Prunus necrotic ringspot virus using magnetic nanoparticle-assisted reverse transcription loop-mediated isothermal amplification.

PubMed

Zong, Xiaojuan; Wang, Wenwen; Wei, Hairong; Wang, Jiawei; Chen, Xin; Xu, Li; Zhu, Dongzi; Tan, Yue; Liu, Qingzhong

2014-11-01

Prunus necrotic ringspot virus (PNRSV) has seriously reduced the yield of Prunus species worldwide. In this study, a highly efficient and specific two-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed to detect PNRSV. Total RNA was extracted from sweet cherry leaf samples using a commercial kit based on a magnetic nanoparticle technique. Transcripts were used as the templates for the assay. The results of this assay can be detected using agarose gel electrophoresis or by assessing in-tube fluorescence after adding SYBR Green I. The assay is highly specific for PNRSV, and it is more sensitive than reverse-transcription polymerase chain reaction (RT-PCR). Restriction enzyme digestion verified further the reliability of this RT-LAMP assay. To our knowledge, this is the first report of the application of RT-LAMP to PNRSV detection in Prunus species. Copyright © 2014 Elsevier B.V. All rights reserved.

Development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis.

PubMed

Ahmed, Mohamed E; Eldigail, Mawahib H; Elamin, Fatima M; Ali, Ibtisam A; Grobusch, Martin P; Aradaib, Imadeldin E

2016-09-13

Cystic echinococcosis (CE) or hydatidosis, caused by the larval stage of Echinococcus granulosus (EG)-complex, is a neglected parasitic disease of public health importance. The disease is endemic in many African and Mediterranean countries including the Sudan. The objective of the present study was to develop and evaluate a real-time loop-mediated isothermal amplification (LAMP) assay for simple and rapid detection of CE in humans and domestic live stock in Sudan. A set of six LAMP primers, designed from the mitochondrial NADH-1 gene of EG cattle strain of genotype 5 (G5), was used as a target for LAMP assay. The assay was performed at a constant temperature (63 °C), with a real-time follow-up using a LightCycler and fluorochrome dye. Following amplification cycles in a simple water bath, LAMP products were observed for color change by naked eye and were visualized under UV light source using agarose gel electrophoresis. The real-time LAMP assay identified a variety of hydatid cysts strains recovered in the Sudan, including Echinococcus canadenses (G6) and Echinococcus ortleppi (G5). Real-time LAMP positive results were detected by the presence of an amplification curve, whereas negative results were indicated by absence of fluorescence detection. Positive LAMP results appeared as a bluish-colored reaction as observed by naked eye, whereas negative LAMP results were observed as purple-colored reaction. The sensitivity studies indicated that the LAMP assay detected as little as a 10 fg of parasite DNA. There was 100 % agreement between results of the LAMP assay and our previously described nested PCR when testing 10-fold serial dilution of DNA extracted from EG-complex hydatid cyst. However, there was no cross-reactivity with other parasites including cysticercus bovis, Fasciola gigantica, and Schistosoma bovis and nucleic acid free samples. The developed LAMP assay would be expected to prove highly significant in epidemiological surveys of CE in developing countries or areas of resource-poor settings for both ease of use and cost.

Use of T12 lighting systems in retrofit applications within New York Office of Mental Health Facilities - A case history

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henry, C.P.; Marsh, E.J.

1997-06-01

In 1990, the Governor of New York State issued Executive Order No. 132, directing all state agencies to reduce energy consumption by 20% from the base year of 1988/89 by the year 2000. To assist in meeting this goal, the New York State Office of Mental Health (OMH) established the Lighting Revitalization Program in 1992. State facilities are divided into five regions, each served by existing Environmental Revitalization Teams. OMH supplemented these teams with lighting technicians in this new program. The program`s goal was to rehabilitate outdated, inefficient lighting systems throughout 28 OMH facilities, totaling 28 million square feet inmore » area. OMH requested the former Facility Development Corporation (FDC), now the Dormitory Authority of the State of New York (DASNY), to contract with Novus Engineering to evaluate the relative efficiency of T8 and T12 ballasts. Novus contracted an independent laboratory, Eastern Testing Laboratories (ETL), for performance testing. ETL tested four ballast/lamp configurations for light Output and input power, and Novus analyzed the results for relative efficiency and also calculated 25-year life cycle costs. The test results indicated that the efficiencies of the T12/34W and T8/32W ballast/lamp technologies were nearly identical. The input power and light output of these systems were similar. The lumens per Watt ratings for the two systems were nearly equal, with the T8 technology being only about two percent more efficient, generating more light with similar input power. The life cycle costs for the two systems were nearly identical, with the T12 system providing a slightly lower life cycle cost. Given the above considerations, the agency has been installing T12 electronic ballasts and 34W lamps in buildings where fluorescent fixtures warranted upgrading. This type of retrofit goes against current trends, but the use of T8 system could not be justified in buildings undergoing minor retrofitting.« less

Switching on fluorescence for selective visual recognition of naringenin and morin with a metal-organic coordination polymer of Zn(bix) [bix = 1,4-bis(imidazol-1-ylmethyl)benzene

NASA Astrophysics Data System (ADS)

Zhao, Xi Juan; Wang, Hui Juan; Liang, Li Jiao; Li, Yuan Fang

2013-02-01

Flavonoids such as naringenin and morin are ubiquitous in a wide range of foods isolated from plants, and have diverse effects on plants even on human health. Here, we establish a selective visual method for recognition of aringenin and morin based on the "switched on" fluorescence induced by a metal-organic coordination polymer of Zn(bix) [bix = 1,4-bis(imidazol-1-ylmethyl)benzene]. Owing to the coordination interaction of aringenin and morin with Zn(II) from the polymeric structure of Zn(bix), the conformational free rotation of naringenin and morin is restricted leading to relatively rigid structures. And as a consequence, the fluorescence is switched on. While luteolin and quercetin, holding a very similar structure with naringenin and morin, have no such fluorescence enhancement most likely owing to the 3'-hydroxy substitution in the B ring. Under 365 nm UV lamp light, we can visually recognize and discriminate naringenin and morin from them each other and luteolin as well as quercetin based on the colors of their emission. With this recognition system, the detection of naringenin and morin in human urine was made with satisfactory results.

Neutrophil extracellular trap formation is associated with autophagy-related signalling in ANCA-associated vasculitis

PubMed Central

Tang, S; Zhang, Y; Yin, S-W; Gao, X-J; Shi, W-W; Wang, Y; Huang, X; Wang, L; Zou, L-Y; Zhao, J-H; Huang, Y-J; Shan, L-Y; Gounni, A S; Wu, Y-Z; Zhang, J-B

2015-01-01

Increasing evidence indicates that aberrant neutrophil extracellular trap (NET) formation could contribute to the pathogenesis of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Recent research has provided evidence that a novel type of ANCA autoantibody, anti-lysosomal membrane protein-2 (LAMP-2) antibody, may have a pathogenic role in AAV. We have shown previously that anti-LAMP-2 antibody-stimulated NET formation contains autoantigens and anti-microbial peptides. The current study sought to determine whether LAMP-2, as a novel antigen of ANCA, was present on NETs in AAV patients, the influence of the anti-LAMP-2 antibody on the neutrophil apoptosis rate and the role of autophagy in anti-LAMP-2 antibody-induced NET formation. NET formation was assessed using immunofluorescence microscopy, scanning electron microscopy or live cell imaging. The neutrophil apoptosis rate was analysed using fluorescence activated cell sorting (FACS). Autophagy was detected using LC3B accumulation and transmission electron microscopy. The results showed that enhanced NET formation, which contains LAMP-2, was observed in kidney biopsies and neutrophils from AAV patients. The apoptosis rate decreased significantly in human neutrophils stimulated with anti-LAMP-2 antibody, and this effect was attenuated by the inhibitors of autophagy 3-methyladenine (3MA) and 2-morpholin-4-yl-8-phenylchromen-4-one (LY294002). The anti-LAMP-2 antibody-stimulated NET formation was unaffected by benzyloxycarbonyl-Val- Ala-Asp (OMe)-fluoromethylketone (zVAD-fmk) and necrostatin-1 (Nec-1), which are inhibitors of apoptosis and necrosis, respectively, but was inhibited by 3MA and LY294002. Moreover, the proportion of LC3BI that was converted to LC3BII increased significantly (P = 0·0057), and massive vacuolizations that exhibited characteristics typical of autophagy were detected in neutrophils stimulated with anti-LAMP-2 antibody. Our results provide further evidence that autophagy is involved in ANCA-induced NET formation in human neutrophils. PMID:25644394

Neutrophil extracellular trap formation is associated with autophagy-related signalling in ANCA-associated vasculitis.

PubMed

Tang, S; Zhang, Y; Yin, S-W; Gao, X-J; Shi, W-W; Wang, Y; Huang, X; Wang, L; Zou, L-Y; Zhao, J-H; Huang, Y-J; Shan, L-Y; Gounni, A S; Wu, Y-Z; Zhang, J-B

2015-06-01

Increasing evidence indicates that aberrant neutrophil extracellular trap (NET) formation could contribute to the pathogenesis of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Recent research has provided evidence that a novel type of ANCA autoantibody, anti-lysosomal membrane protein-2 (LAMP-2) antibody, may have a pathogenic role in AAV. We have shown previously that anti-LAMP-2 antibody-stimulated NET formation contains autoantigens and anti-microbial peptides. The current study sought to determine whether LAMP-2, as a novel antigen of ANCA, was present on NETs in AAV patients, the influence of the anti-LAMP-2 antibody on the neutrophil apoptosis rate and the role of autophagy in anti-LAMP-2 antibody-induced NET formation. NET formation was assessed using immunofluorescence microscopy, scanning electron microscopy or live cell imaging. The neutrophil apoptosis rate was analysed using fluorescence activated cell sorting (FACS). Autophagy was detected using LC3B accumulation and transmission electron microscopy. The results showed that enhanced NET formation, which contains LAMP-2, was observed in kidney biopsies and neutrophils from AAV patients. The apoptosis rate decreased significantly in human neutrophils stimulated with anti-LAMP-2 antibody, and this effect was attenuated by the inhibitors of autophagy 3-methyladenine (3MA) and 2-morpholin-4-yl-8-phenylchromen-4-one (LY294002). The anti-LAMP-2 antibody-stimulated NET formation was unaffected by benzyloxycarbonyl-Val- Ala-Asp (OMe)-fluoromethylketone (zVAD-fmk) and necrostatin-1 (Nec-1), which are inhibitors of apoptosis and necrosis, respectively, but was inhibited by 3MA and LY294002. Moreover, the proportion of LC3BI that was converted to LC3BII increased significantly (P=0.0057), and massive vacuolizations that exhibited characteristics typical of autophagy were detected in neutrophils stimulated with anti-LAMP-2 antibody. Our results provide further evidence that autophagy is involved in ANCA-induced NET formation in human neutrophils. © 2015 British Society for Immunology.

GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use.

PubMed

Kiddle, Guy; Hardinge, Patrick; Buttigieg, Neil; Gandelman, Olga; Pereira, Clint; McElgunn, Cathal J; Rizzoli, Manuela; Jackson, Rebecca; Appleton, Nigel; Moore, Cathy; Tisi, Laurence C; Murray, James A H

2012-04-30

There is an increasing need for quantitative technologies suitable for molecular detection in a variety of settings for applications including food traceability and monitoring of genetically modified (GM) crops and their products through the food processing chain. Conventional molecular diagnostics utilising real-time polymerase chain reaction (RT-PCR) and fluorescence-based determination of amplification require temperature cycling and relatively complex optics. In contrast, isothermal amplification coupled to a bioluminescent output produced in real-time (BART) occurs at a constant temperature and only requires a simple light detection and integration device. Loop mediated isothermal amplification (LAMP) shows robustness to sample-derived inhibitors. Here we show the applicability of coupled LAMP and BART reactions (LAMP-BART) for determination of genetically modified (GM) maize target DNA at low levels of contamination (0.1-5.0% GM) using certified reference material, and compare this to RT-PCR. Results show that conventional DNA extraction methods developed for PCR may not be optimal for LAMP-BART quantification. Additionally, we demonstrate that LAMP is more tolerant to plant sample-derived inhibitors, and show this can be exploited to develop rapid extraction techniques suitable for simple field-based qualitative tests for GM status determination. We also assess the effect of total DNA assay load on LAMP-BART quantitation. LAMP-BART is an effective and sensitive technique for GM detection with significant potential for quantification even at low levels of contamination and in samples derived from crops such as maize with a large genome size. The resilience of LAMP-BART to acidic polysaccharides makes it well suited to rapid sample preparation techniques and hence to both high throughput laboratory settings and to portable GM detection applications. The impact of the plant sample matrix and genome loading within a reaction must be controlled to ensure quantification at low target concentrations.

LIGHTING FOR READING: DESIGNING AN LED LUMINAIRE FOR HOMES AND OFFICES

EPA Science Inventory

Energy waste from traditional incandescent light bulbs was reduced by fluorescent lamps, but they pose a threat to the environment due to the mercury in each tube and disposal issues. Light emitting diodes (LEDs) provide superior energy efficiency, longer life, toxin-free comp...

75 FR 22213 - Energy Conservation Program: Test Procedures for General Service Fluorescent Lamps, Incandescent...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-28

.... Since the publication of that rule, it has come to DOE's attention that, due to a technical oversight, a...-percent confidence limit of the true mean (X L ) divided by 0.97, i.e., [GRAPHIC] [TIFF OMITTED] TR28AP10...

Solid-State Lighting. Early Lessons Learned on the Way to Market

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sandahl, L. J.; Cort, K. A.; Gordon, K. L.

2014-01-01

Analysis of issues and lessons learned during the early stages of solid-state lighting market introduction in the U.S., which also summarizes early actions taken to avoid potential problems anticipated based on lessons learned from the market introduction of compact fluorescent lamps.

Modification of measurement methods for evaluation of tissue-engineered cartilage function and biochemical properties using nanosecond pulsed laser

NASA Astrophysics Data System (ADS)

Ishihara, Miya; Sato, Masato; Kutsuna, Toshiharu; Ishihara, Masayuki; Mochida, Joji; Kikuchi, Makoto

2008-02-01

There is a demand in the field of regenerative medicine for measurement technology that enables determination of functions and components of engineered tissue. To meet this demand, we developed a method for extracellular matrix characterization using time-resolved autofluorescence spectroscopy, which enabled simultaneous measurements with mechanical properties using relaxation of laser-induced stress wave. In this study, in addition to time-resolved fluorescent spectroscopy, hyperspectral sensor, which enables to capture both spectral and spatial information, was used for evaluation of biochemical characterization of tissue-engineered cartilage. Hyperspectral imaging system provides spectral resolution of 1.2 nm and image rate of 100 images/sec. The imaging system consisted of the hyperspectral sensor, a scanner for x-y plane imaging, magnifying optics and Xenon lamp for transmmissive lighting. Cellular imaging using the hyperspectral image system has been achieved by improvement in spatial resolution up to 9 micrometer. The spectroscopic cellular imaging could be observed using cultured chondrocytes as sample. At early stage of culture, the hyperspectral imaging offered information about cellular function associated with endogeneous fluorescent biomolecules.

Effect of UV irradiation on the apoptosis and necrosis of Jurkat cells using UV LEDs

NASA Astrophysics Data System (ADS)

Inada, Shunko A.; Amano, Hiroshi; Akasaki, Isamu; Morita, Akimichi; Kobayashi, Keiko

2009-02-01

Phototherapy is a very effective method for treating most of the incurable skin diseases. A fluorescent light bulb is used as a conventional UV light source for this type of therapy. However, infrared radiation from the light source sometimes causes serious problems on patient's health. In addition, the normal part of the skin is irradiated when a large fluorescent light bulb is used. Moreover, a conventional UV irradiation system is heavy and has a short lifetime and a high electrical power consumption. Therefore, a new UV light source for solving the problems of phototherapy is required. To realize low-power-consumption, lightweight and long-lifetime systems, group III nitride-based UV-A1 light-emitting diodes (LEDs) were investigated. We examined the UV LED irradiation of Jurkat cell, which is a tumor cell and more sensitive to UV light than a healthy cell. The numbers of apoptotic and necrotic cells were confirmed to be the same using a UV LED and a conventional lamp system. The UV LED showed the possibility of realizing a new UV light source for phototherapy.

Sole-Source Lighting for Controlled-Environment Agriculture

NASA Technical Reports Server (NTRS)

Mitchell.Cary; Stutte, Gary W.

2015-01-01

Since plants on Earth evolved under broad-spectrum solar radiation, anytime they are grown exclusively under electric lighting that does not contain all wavelengths in similar proportion to those in sunlight, plant appearance and size could be uniquely different. Nevertheless, plants have been grown for decades under fluorescent (FL) (1) + incandescent (IN) (2) lamps as a sole source of lighting (SSL), and researchers have become comfortable that, in certain proportions of FL + IN for a given species, plants can appear "normal" relative to their growth outdoors. The problem with using such traditional SSLs for commercial production typically is short lamp lifespans and not obtaining enough photosynthetically active radiation (PAR, 400-700 nm) when desired. These limitations led to supplementation of FL + IN lamp outputs with longer-lived, high-intensity discharge (HID) lamps in growth chambers (3). As researchers became comfortable that mixes of orange-biased high-pressure sodium (HPS) and blue-biased metal halide (MH) HIDs together also could give normal plant growth at higher intensities, growth chambers and phytotrons subsequently were equipped mainly with HID lamps, with their intense thermal output filtered out by ventilated light caps or thermal-controlled water barriers. For the most part, IN and HID lamps have found a home in commercial protected horticulture, usually for night-break photoperiod lighting (IN) or for seasonal supplemental lighting (mostly HPS) in greenhouses. However, lack of economically viable options for SSL have held back aspects of year-round indoor agriculture from taking off commercially.

Ratiometric fluorescent nanosensor based on carbon dots for the detection of mercury ion

NASA Astrophysics Data System (ADS)

Ma, Yusha; Mei, Jing; Bai, Jianliang; Chen, Xu; Ren, Lili

2018-05-01

A novel ratiometric fluorescent nanosensor based on carbon dots has been synthesized via bonding rhodamine B hydrazide to the carbon dots surface by an amide reaction. The ratiometric fluorescent nanosensor showed only a single blue fluorescence emission around 450 nm. While, as mercury ion was added, due to the open-ring of rhodamine moiety bonded on the CDs surface, the orange emission of the open-ring rhodamine would increase obviously according to the concentration of mercury ion, resulting in the distinguishable dual emissions at 450 nm and 575 nm under a single 360 excitation wavelength. Meanwhile, the ratiometric fluorescent nanosensor based on carbon dots we prepared is more sensitive to qualitative and semi-quantitative detection of mercury ion in the range of 0–100 μM, because fluorescence changes gradually from blue to orange emission under 365 nm lamp with the increasing of mercury ion in the tested solution.

Evaluation of an LED Retrofit Project at Princeton University’s Carl Icahn Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Davis, Robert G.; Murphy, Arthur L.; Perrin, Tess E.

The LED lighting retrofit at the Carl Icahn Laboratory of the Lewis-Sigler Institute for Integrative Genomics was the first building-wide interior LED project at Princeton University, following the University’s experiences from several years of exterior and small-scale interior LED implementation projects. The project addressed three luminaire types – recessed 2x2 troffers, cove and other luminaires using linear T8 fluorescent lamps, and CFL downlights - which combined accounted for over 564,000 kWh of annual energy, over 90% of the lighting energy used in the facility. The Princeton Facilities Engineering staff used a thorough process of evaluating product alternatives before selecting anmore » acceptable LED retrofit solution for each luminaire type. Overall, 815 2x2 luminaires, 550 linear fluorescent luminaires, and 240 downlights were converted to LED as part of this project. Based solely on the reductions in wattage in converting from the incumbent fluorescent lamps to LED retrofit kits, the annual energy savings from the project was over 190,000 kWh, a savings of 37%. An additional 125,000 kWh of energy savings is expected from the implementation of occupancy and task-tuning control solutions, which will bring the total savings for the project to 62%.« less

Time-synchronized continuous wave laser-induced fluorescence on an oscillatory xenon discharge.

PubMed

MacDonald, N A; Cappelli, M A; Hargus, W A

2012-11-01

A novel approach to time-synchronizing laser-induced fluorescence measurements to an oscillating current in a 60 Hz xenon discharge lamp using a continuous wave laser is presented. A sample-hold circuit is implemented to separate out signals at different phases along a current cycle, and is followed by a lock-in amplifier to pull out the resulting time-synchronized fluorescence trace from the large background signal. The time evolution of lower state population is derived from the changes in intensity of the fluorescence excitation line shape resulting from laser-induced fluorescence measurements of the 6s(')[1/2](1)(0)-6p(')[3/2](2) xenon atomic transition at λ = 834.68 nm. Results show that the lower state population oscillates at twice the frequency of the discharge current, 120 Hz.

Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a highly sensitive detection of enterovirus in the stool samples of acute flaccid paralysis cases

PubMed Central

2009-01-01

Background In the global eradication program for poliomyelitis, the laboratory diagnosis plays a critical role by isolating poliovirus (PV) from the stool samples of acute flaccid paralysis (AFP) cases. In this study, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system for a rapid and highly sensitive detection of enterovirus including PV to identify stool samples positive for enterovirus including PV. Methods A primer set was designed for RT-LAMP to detect enterovirus preferably those with PV-like 5'NTRs of the viral genome. The sensitivity of RT-LAMP system was evaluated with prototype strains of enterovirus. Detection of enterovirus from stool extracts was examined by using RT-LAMP system. Results We detected at least 400 copies of the viral genomes of PV(Sabin) strains within 90 min by RT-LAMP with the primer set. This RT-LAMP system showed a preference for Human enterovirus species C (HEV-C) strains including PV, but exhibited less sensitivity to the prototype strains of HEV-A and HEV-B (detection limits of 7,400 to 28,000 copies). Stool extracts, from which PV, HEV-C, or HEV-A was isolated in the cell culture system, were mostly positive by RT-LAMP method (positive rates of 15/16 (= 94%), 13/14 (= 93%), and 4/4 (= 100%), respectively). The positive rate of this RT-LAMP system for stool extracts from which HEV-B was isolated was lower than that of HEV-C (positive rate of 11/21 (= 52%)). In the stool samples, which were negative for enterovirus isolation by the cell culture system, we found that two samples were positive for RT-LAMP (positive rates of 2/38 (= 5.3%)). In these samples, enterovirus 96 was identified by sequence analysis utilizing a seminested PCR system. Conclusions RT-LAMP system developed in this study showed a high sensitivity comparable to that of the cell culture system for the detection of PV, HEV-A, and HEV-C, but less sensitivity to HEV-B. This RT-LAMP system would be useful for the direct detection of enterovirus from the stool extracts. PMID:20015403

10 CFR 430.3 - Materials incorporated by reference.

Code of Federal Regulations, 2010 CFR

2010-01-01

.... Department of Energy, Office of Energy Efficiency and Renewable Energy, Building Technologies Program, 6th... Standard for Fluorescent Lamps-Instant-start and Cold-Cathode Types-Dimensional and Electrical...-1995 (“ANSI C78.20”), American National Standard for electric lamps—A, G, PS, and Similar Shapes with...

75 FR 14319 - Energy Conservation Standards for Fluorescent Lamp Ballasts: Public Meeting and Availability of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-24

... Technical Support Document AGENCY: Office of Energy Efficiency and Renewable Energy, Department of Energy. ACTION: Notice of public meeting and availability of preliminary technical support document. SUMMARY: The... products. DOE encourages written comments on these subjects. To inform interested parties and facilitate...

Lighting Systems For High Speed Photography Applying Special Metal Halide Discharge Lamps

NASA Astrophysics Data System (ADS)

Gillum, Keith M.; Steuernagel, K. H.

1983-03-01

High speed photography requires, in addition to a good color quality of the light source, a very high level of illumination. Conventional lighting systems utilizing incandescent lamps or other metal halide lamp types has inherent problems of inefficient light output or poor color quality. Heat generated by incandescent lamps and the power these sources require drive up operating and installation costs. A most economical and practical solution was devised by using the metal halide discharge lamp developed by OSRAM, GmbH of Munich, West Germany. This lamp trade marked the HMITM Metallogen was primarily developed for the needs of the television and motion picture film industry. Due to their high efficiency and other consistent operating qualities these lamps also fulfill the needs of high speed photography, e.g. in crash test facilities, when special engineering activities are carried out. The OSRAM HMITM lamp is an AC discharge metal halide lamp with rare earth additives to increase both the efficiency and light output qualities. Since the lamp is an AC source, a special method had to be developed to overcome the strobing effect, which is normal for AC lamps given their modulated light output, when used with high speed cameras, (e.g. with >1000 fps). This method is based on an increased frequency for the lamp supply voltage coupled with a mix of the light output achieved using a multiphase mains power supply. First developed in 1977, this system using the OSRAM HMITM lamps was installed in a crash test facility of a major automotive manufacturer in West Germany. The design resulted in the best lighting and performance ever experienced. Since that time several other motor companies have made use of this breakthrough. Industrial and scientific users are now considering additional applications use of this advanced high speed lighting system.

Identification of lanthanum-specific peptides for future recycling of rare earth elements from compact fluorescent lamps.

PubMed

Lederer, Franziska L; Curtis, Susan B; Bachmann, Stefanie; Dunbar, W Scott; MacGillivray, Ross T A

2017-05-01

As components of electronic scrap, rare earth minerals are an interesting but little used source of raw materials that are highly important for the recycling industry. Currently, there exists no cost-efficient technology to separate rare earth minerals from an electronic scrap mixture. In this study, phage surface display has been used as a key method to develop peptides with high specificity for particular inorganic targets in electronic scrap. Lanthanum phosphate doped with cerium and terbium as part of the fluorescent phosphors of spent compact fluorescent lamps (CFL) was used as a target material of economic interest to test the suitability of the phage display method to the separation of rare earth minerals. One random pVIII phage library was screened for peptide sequences that bind specifically to the fluorescent phosphor LaPO 4 :Ce 3+ ,Tb 3+ (LAP). The library contained at least 100 binding pVIII peptides per phage particle with a diversity of 1 × 10 9 different phage per library. After three rounds of enrichment, a phage clone containing the surface peptide loop RCQYPLCS was found to bind specifically to LAP. Specificity and affinity of the identified phage bound peptide was confirmed by using binding and competition assays, immunofluorescence assays, and zeta potential measurements. Binding and immunofluorescence assays identified the peptide's affinity for the fluorescent phosphor components CAT (CeMgAl 11 O 19 :Tb 3+ ) and BAM (BaMgAl 10 O 17 :Eu 2+ ). No affinity was found for other fluorescent phosphor components such as YOX (Y 2 O 3 :Eu 3+ ). The binding specificity of the RCQYPLCS peptide loop was improved 3-51-fold by using alanine scanning mutagenesis. The identification of peptides with high specificity and affinity for special components in the fluorescent phosphor in CFLs provides a potentially new strategic approach to rare earth recycling. Biotechnol. Bioeng. 2017;114: 1016-1024. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Recent developments in optical detection methods for microchip separations.

PubMed

Götz, Sebastian; Karst, Uwe

2007-01-01

This paper summarizes the features and performances of optical detection systems currently applied in order to monitor separations on microchip devices. Fluorescence detection, which delivers very high sensitivity and selectivity, is still the most widely applied method of detection. Instruments utilizing laser-induced fluorescence (LIF) and lamp-based fluorescence along with recent applications of light-emitting diodes (LED) as excitation sources are also covered in this paper. Since chemiluminescence detection can be achieved using extremely simple devices which no longer require light sources and optical components for focusing and collimation, interesting approaches based on this technique are presented, too. Although UV/vis absorbance is a detection method that is commonly used in standard desktop electrophoresis and liquid chromatography instruments, it has not yet reached the same level of popularity for microchip applications. Current applications of UV/vis absorbance detection to microchip separations and innovative approaches that increase sensitivity are described. This article, which contains 85 references, focuses on developments and applications published within the last three years, points out exciting new approaches, and provides future perspectives on this field.

Loop-mediated isothermal amplification as an emerging technology for detection of Yersinia ruckeri the causative agent of enteric red mouth disease in fish.

PubMed

Saleh, Mona; Soliman, Hatem; El-Matbouli, Mansour

2008-08-12

Enteric Redmouth (ERM) disease also known as Yersiniosis is a contagious disease affecting salmonids, mainly rainbow trout. The causative agent is the gram-negative bacterium Yersinia ruckeri. The disease can be diagnosed by isolation and identification of the causative agent, or detection of the Pathogen using fluorescent antibody tests, ELISA and PCR assays. These diagnostic methods are laborious, time consuming and need well trained personnel. A loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for detection of Y. ruckeri the etiological agent of enteric red mouth (ERM) disease in salmonids. The assay was optimised to amplify the yruI/yruR gene, which encodes Y. ruckeri quorum sensing system, in the presence of a specific primer set and Bst DNA polymerase at an isothermal temperature of 63 degrees C for one hour. Amplification products were detected by visual inspection, agarose gel electrophoresis and by real-time monitoring of turbidity resulted by formation of LAMP amplicons. Digestion with HphI restriction enzyme demonstrated that the amplified product was unique. The specificity of the assay was verified by the absence of amplification products when tested against related bacteria. The assay had 10-fold higher sensitivity compared with conventional PCR and successfully detected Y. ruckeri not only in pure bacterial culture but also in tissue homogenates of infected fish. The ERM-LAMP assay represents a practical alternative to the microbiological approach for rapid, sensitive and specific detection of Y. ruckeri in fish farms. The assay is carried out in one hour and needs only a heating block or water bath as laboratory furniture. The advantages of the ERM-LAMP assay make it a promising tool for molecular detection of enteric red mouth disease in fish farms.

Technology for radiation efficiency measurement of high-power halogen tungsten lamp used in calibration of high-energy laser energy meter.

PubMed

Wei, Ji Feng; Hu, Xiao Yang; Sun, Li Qun; Zhang, Kai; Chang, Yan

2015-03-20

The calibration method using a high-power halogen tungsten lamp as a calibration source has many advantages such as strong equivalence and high power, so it is very fit for the calibration of high-energy laser energy meters. However, high-power halogen tungsten lamps after power-off still reserve much residual energy and continually radiate energy, which is difficult to be measured. Two measuring systems were found to solve the problems. One system is composed of an integrating sphere and two optical spectrometers, which can accurately characterize the radiative spectra and power-time variation of the halogen tungsten lamp. This measuring system was then calibrated using a normal halogen tungsten lamp made of the same material as the high-power halogen tungsten lamp. In this way, the radiation efficiency of the halogen tungsten lamp after power-off can be quantitatively measured. In the other measuring system, a wide-spectrum power meter was installed far away from the halogen tungsten lamp; thus, the lamp can be regarded as a point light source. The radiation efficiency of residual energy from the halogen tungsten lamp was computed on the basis of geometrical relations. The results show that the halogen tungsten lamp's radiation efficiency was improved with power-on time but did not change under constant power-on time/energy. All the tested halogen tungsten lamps reached 89.3% of radiation efficiency at 50 s after power-on. After power-off, the residual energy in the halogen tungsten lamp gradually dropped to less than 10% of the initial radiation power, and the radiation efficiency changed with time. The final total radiation energy was decided by the halogen tungsten lamp's radiation efficiency, the radiation efficiency of residual energy, and the total power consumption. The measuring uncertainty of total radiation energy was 2.4% (here, the confidence factor is two).

A new relative referencing method for crop monitoring using chlorophyll fluorescence

NASA Technical Reports Server (NTRS)

Norikane, J.; Goto, E.; Kurata, K.; Takakura, T.

2003-01-01

The measurement of plant chlorophyll fluorescence has been used for many years as a method to monitor a plant's health status. These types of methods have been mostly relegated to the laboratory. The newly developed Relative Referencing Method allows for the measurement of chlorophyll fluorescence under artificial lighting conditions. The fluorescence signal can be determined by first taking a reference signal measurement, then a second measurement with an additional fluorescence excitation source. The first signal can then be subtracted from the second and the plant's chlorophyll fluorescence due to the second lighting source can be determined. With this simple approach, a photosynthesizing plant can be monitored to detect signs of water stress. Using this approach experiments on tomato plants have shown that it was possible to detect water stress, while the plants were continuously illuminated by fluorescent lamps. This method is a promising tool for the remote monitoring of crops grown in a CELSS-type application. Published by Elsevier Science Ltd on behalf of COSPAR.

Shining a light on LAMP assays--a comparison of LAMP visualization methods including the novel use of berberine.

PubMed

Fischbach, Jens; Xander, Nina Carolin; Frohme, Marcus; Glökler, Jörn Felix

2015-04-01

The need for simple and effective assays for detecting nucleic acids by isothermal amplification reactions has led to a great variety of end point and real-time monitoring methods. Here we tested direct and indirect methods to visualize the amplification of potato spindle tuber viroid (PSTVd) by loop-mediated isothermal amplification (LAMP) and compared features important for one-pot in-field applications. We compared the performance of magnesium pyrophosphate, hydroxynaphthol blue (HNB), calcein, SYBR Green I, EvaGreen, and berberine. All assays could be used to distinguish between positive and negative samples in visible or UV light. Precipitation of magnesium-pyrophosphate resulted in a turbid reaction solution. The use of HNB resulted in a color change from violet to blue, whereas calcein induced a change from orange to yellow-green. We also investigated berberine as a nucleic acid-specific dye that emits a fluorescence signal under UV light after a positive LAMP reaction. It has a comparable sensitivity to SYBR Green I and EvaGreen. Based on our results, an optimal detection method can be chosen easily for isothermal real-time or end point screening applications.

Experimental ultraviolet photocarcinogenesis: wavelength interactions and time-dose relationships.

PubMed

Forbes, P D; Davies, R E; Urbach, F

1978-12-01

Tumors were induced in the skin of SKH hairless mice by exposure to fluorescent FS sun lamps or to a long-arc xenon solar simulator. Tumores developed about equally well with varying amounts of UV-A radiation (lambda greater than 320 nm) given simultaneously. In contrast, incremental changes in the UV-B region (lambda less than 320 nm) led to substantial increases in carcinogenic effectiveness. A tumor-"initiating" dose of UV-B (4-10 wk of daily FS lamp exposures) was rendered less effective by subsequent exposures of the mice to UV-A (6 hr/day, F-40 T12BL lamps). The mechanism for this effect is not known. Most tumors induced by a short course (10 wk) of FS lamp exposure grew slowly or regressed, whereas mice exposed for a longer period (30 wk) developed more tumors, and many of those that appeared early grew aggressively. Effects of daily dose fractionation were less clear, and the subject requires further study. These and other variables are being tested in a program designed to yield useful information on the effects of changing spectrum, dose, and dose delivery rates on sunlight-induced cancer.

UV exposure in artificial and natural weathering: A comparative study

NASA Astrophysics Data System (ADS)

Heikkilä, A.; Kazadzis, S.; Meinander, O.; Vaskuri, A.; Kärhä, P.; Mylläri, V.; Syrjälä, S.; Koskela, T.

2017-02-01

We report on a study focusing on UV exposure conditions in three different types of chambers used for accelerated ageing of materials. The first chamber is equipped with four 300-W UVA/UVB mercury vapour lamps (Ultra-Vitalux/Osram). The second chamber uses four 40-W UVA fluorescent lamps (QUV-340/Q-Lab). The third chamber is Weather-Ometer Ci3000+ from Atlas with a 4500-W xenon arc lamp. UV irradiance prevailing in each chamber was measured using Bentham DM150 double monochromator spectroradiometer. The results were compared to measurements of solar spectral UV irradiance at Jokioinen, Finland, with a Brewer MkIII double monochromator spectrophotometer. The spectral shapes of the exposing UV radiation in the different chambers were found to notably differ from each other and from the solar UV spectrum. Both spatial inhomogeneities and temporal variability caused by various factors, like the ageing of the lamps, were detected. The effects were found to strongly depend on wavelength of the exposing UV radiation. The findings of this study underline the necessity of careful characterization of the UV exposure conditions provided by the facilities used in accelerated testing of materials.

Reliability of a visual scoring system with fluorescent tracers to assess dermal pesticide exposure.

PubMed

Aragon, Aurora; Blanco, Luis; Lopez, Lylliam; Liden, Carola; Nise, Gun; Wesseling, Catharina

2004-10-01

We modified Fenske's semi-quantitative 'visual scoring system' of fluorescent tracer deposited on the skin of pesticide applicators and evaluated its reproducibility in the Nicaraguan setting. The body surface of 33 farmers, divided into 31 segments, was videotaped in the field after spraying with a pesticide solution containing a fluorescent tracer. A portable UV lamp was used for illumination in a foldaway dark room. The videos of five farmers were randomly selected. The scoring was based on a matrix with extension of fluorescent patterns (scale 0-5) on the ordinate and intensity (scale 0-5) on the abscissa, with the product of these two ranks as the final score for each body segment (0-25). Five medical students rated and evaluated the quality of 155 video images having undergone 4 h of training. Cronbach alpha coefficients and two-way random effects intraclass correlation coefficients (ICC) with absolute agreement were computed to assess inter-rater reliability. Consistency was high (Cronbach alpha = 0.96), but the scores differed substantially between raters. The overall ICC was satisfactory [0.75; 95% confidence interval (CI) = 0.62-0.83], but it was lower for intensity (0.54; 95% CI = 0.40-0.66) and higher for extension (0.80; 95% CI = 0.71-0.86). ICCs were lowest for images with low scores and evaluated as low quality, and highest for images with high scores and high quality. Inter-rater reliability coefficients indicate repeatability of the scoring system. However, field conditions for recording fluorescence should be improved to achieve higher quality images, and training should emphasize a better mechanism for the reading of body areas with low contamination.

49 CFR 393.23 - Power supply for lamps.

Code of Federal Regulations, 2014 CFR

2014-10-01

... NECESSARY FOR SAFE OPERATION Lamps, Reflective Devices, and Electrical Wiring § 393.23 Power supply for lamps. All required lamps must be powered by the electrical system of the motor vehicle with the...

Optical Diagnostics in the Gaseous Electronics Conference Reference Cell

PubMed Central

Hebner, G. A.; Greenberg, K. E.

1995-01-01

A number of laser-induced fluorescence and absorption spectroscopy studies have been conducted using Gaseous Electronics Conference Reference Cells. Laser-induced fluorescence has been used to measure hydrogen atom densities, to measure argon metastable spatial profiles, to determine the sheath electric field, and to infer the electron density and temperature. Absorption spectroscopy, using lamp sources and diode lasers, has been used to measure metastable atom densities in helium and argon discharges and fluorocarbon densities in silicon etching discharges. The experimental techniques and sample results of these investigations are reviewed. PMID:29151748

Enchanced methods of hydrophilized CdSe quantum dots synthesis

NASA Astrophysics Data System (ADS)

Potapkin, D. V.; Zharkova, I. S.; Goryacheva, I. Y.

2015-03-01

Quantum dots are bright and stable fluorescence signal sources, but for most of applications they need an additional hydrophilization step. Unfortunately, most of existing approaches lead to QD's fluorescence quenching, so there is a need for additional enhancing of hydrophilized QD's brightness like UV irradiation, which can be used both on water insoluble QD's with oleic acid ligands (in toluene) and on hydrophilized QD's covered with UV-stable polymer (in aqueous solution). For synthesis of bright water-soluble fluorescent labels CdSe/CdS/ZnS colloidal quantum dots were covered with PAMAM dendrimer and irradiated with UV lamp in quartz cuvettes for 3 hours at the room temperature and then compared with control sample.

Slit-lamp photography made easy by a spot metering system.

PubMed Central

Khaw, P T; Elkington, A R

1988-01-01

The use of a standard 35 mm camera with a spot metering system to take slit-lamp photographs is described. This system is mounted on a standard Haag-Streit slit-lamp and can be used with good results even by inexperienced operators. Images PMID:3390424

Color speckle in laser displays

NASA Astrophysics Data System (ADS)

Kuroda, Kazuo

2015-07-01

At the beginning of this century, lighting technology has been shifted from discharge lamps, fluorescent lamps and electric bulbs to solid-state lighting. Current solid-state lighting is based on the light emitting diodes (LED) technology, but the laser lighting technology is developing rapidly, such as, laser cinema projectors, laser TVs, laser head-up displays, laser head mounted displays, and laser headlamps for motor vehicles. One of the main issues of laser displays is the reduction of speckle noise1). For the monochromatic laser light, speckle is random interference pattern on the image plane (retina for human observer). For laser displays, RGB (red-green-blue) lasers form speckle patterns independently, which results in random distribution of chromaticity, called color speckle2).

Defects Induced Enhancement of Eu3+ Emission in Yttria ( Y2O3:Eu3+)

NASA Astrophysics Data System (ADS)

Jagannathan, R.; Kutty, T.; Kottaisamy, M.; Jeyagopal, P.

1994-11-01

Bixbyite type Y2O3:Eu3+ apart from being the efficient red phosphor extensively used in trichromatic fluorescent lamps, it is a typical system one can apply Jørgensen's refined electron spin pairing theory. This can be used to explain the enhancement in Eu3+ emission intensity observed with the aliovalent substitution in the yttria host matrix. Results based on these are explained qualitatively by considering a simple configurational coordinate model. Futhermore, an insight into the different types of defects induced with the aliovalent substitution in the yttria lattice has become possible with EPR probe.

Development and Application of a Loop-Mediated Isothermal Amplification (LAMP) Approach for the Rapid Detection of Dirofilaria repens from Biological Samples

PubMed Central

Raele, Donato Antonio; Pugliese, Nicola; Galante, Domenico; Latorre, Laura Maria; Cafiero, Maria Assunta

2016-01-01

Dirofilariasis by Dirofilaria repens is an important mosquito vector borne parasitosis, and the dog represents the natural host and reservoir of the parasite. This filarial nematode can also induce disease in humans, and in the last decades an increasing number of cases have been being reported. The present study describes the first loop mediated isothermal amplification (LAMP) assay to detect D. repens DNA in blood and mosquitoes. Two versions of the technique have been developed and described: in the first, the amplification is followed point by point through a real time PCR instrument (ReT-LAMP); in the second, the amplification is visualized by checking UV fluorescence of the reaction mixture after addition of propidium iodide (PI-LAMP). The two variants use the same set of 4 primers targeting the D. repens cytochrome oxidase subunit I (COI) gene. To assess the specificity of the method, reactions were carried out by using DNA from the major zoonotic parasites of the family of Onchocercidae, and no amplification was observed. The lower limit of detection of the ReT-LAMP assay was 0.15 fg/μl (corresponding to about 50 copy of COI gene per μl). Results suggest that the described assay is specific, and its sensitivity is higher than the conventional PCR based on the same gene. It is also provide a rapid and cost-effective molecular detection of D. repens, mainly when PI-LAMP is applied, and it should be performed in areas where this emerging parasitosis is endemic. PMID:27341205

Production of reactive species using vacuum ultraviolet photodissociation as a tool for studying their effects in plasma medicine: simulations and measurements

NASA Astrophysics Data System (ADS)

Ono, Ryo; Tokumitsu, Yusuke; Zen, Shungo; Yonemori, Seiya

2014-11-01

We propose a method for producing OH, H, O, O3, and O2(a1Δg) using the vacuum ultraviolet photodissociation of H2O and O2 as a tool for studying the reaction processes of plasma medicine. For photodissociation, an H2O/He or O2/He mixture flowing in a quartz tube is irradiated by a Xe2 or Kr2 excimer lamp. The effluent can be applied to a target. Simulations show that the Xe2 lamp method can produce OH radicals within 0.1-1 ppm in the effluent at 5 mm from a quartz tube nozzle. This is comparable to those produced by a helium atmospheric-pressure plasma jet (He-APPJ) currently used in plasma medicine. The Xe2 lamp method also produces H atoms of, at most, 6 ppm. In contrast, the maximum O densities produced by the Xe2 and Kr2 lamp methods are 0.15 ppm and 2.5 ppm, respectively; these are much lower than those from He-APPJ (several tens of ppm). Both lamp methods can produce ozone at concentrations above 1000 ppm and O2(a1Δg) at tens of ppm. The validity of the simulations is verified by measuring the O3 and OH densities produced by the Xe2 lamp method using ultraviolet absorption and laser-induced fluorescence. The differences between the measured and simulated densities for O3 and OH are 20% and factors of 3-4, respectively.

[Imaging of surface cell antigens on the tumor sections of lymph nodes using fluorescence quantum dots].

PubMed

Rafalovskaia-Orlovskaia, E P; Gorgidze, L A; Gladkikh, A A; Tauger, S M; Vorob'ev, I A

2012-01-01

The usefulness of quantum dots for the immunofluorescent detection of surface antigens on the lymphoid cells has been studied. To optimize quantum dots detection we have upgraded fluorescent microscope that allows obtaining multiple images from different quantum dots from one section. Specimens stained with quantum dots remained stable over two weeks and practically did not bleach under mercury lamp illumination during tens of minutes. Direct conjugates of primary mouse monoclonal antibodies with quantum dots demonstrated high specificity and sufficient sensitivity in the case of double staining on the frozen sections. Because of the high stability of quantum dots' fluorescence, this method allows to analyze antigen coexpression on the lymphoid tissue sections for diagnostic purposes. The spillover of fluorescent signals from quantum dots into adjacent fluorescent channels, with maxima differing by 40 nm, did not exceed 8%, which makes the spectral compensation is practically unnecessary.

77 FR 4363 - Certain Dimmable Compact Fluorescent Lamps and Products Containing Same; Receipt of Complaint...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-27

.... of China; Feit Electric Company, Inc. of CA; General Electric Company of CT; Xiamen Topstar Lighting Co. Ltd. of China; Technical Consumer Products, Inc. of OH; TCP China of China; TCP (Shanghai) Tiancanbao Lighting of China; Shanghai Jensing Electron Electrical Equipment Co., Ltd. of China; Shanghai...

76 FR 56661 - Energy Conservation Program: Test Procedures for General Service Fluorescent Lamps, General...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-14

... designed to improve energy efficiency and established the Energy Conservation Program for Consumer Products... prescribed or amended under this section must be reasonably designed to produce test results which measure... conservation standards for GSILs which include for the first time minimum rated lifetime requirements that are...

Demonstrating the Influence of UV Rays on Living Things.

ERIC Educational Resources Information Center

Morimoto, Kouichi

2002-01-01

Describes an experiment that introduces students to the different types of UV rays and their effects on living things by using appropriate teaching materials and equipment. Demonstrates the effects of exposure to UV-B (fluorescent) and UV-C (germicidal) lamps by using bananas, duckweed, and the fruit fly. (Contains 14 references.) (Author/YDS)

76 FR 20089 - Energy Conservation Program: Energy Conservation Standards for Fluorescent Lamp Ballasts

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-11

... = high output. ** The proposed standards are based on an equation that is a function of the natural... High estimate Discount rate (emerging (existing Primary estimate technologies, roll- technologies, up...$) is the average of the low and high values used in DOE's analysis. [dagger] Total Benefits for both...

Improving spinach, radish, and lettuce growth under red light-emitting diodes (LEDs) with blue light supplementation

NASA Technical Reports Server (NTRS)

Yorio, N. C.; Goins, G. D.; Kagie, H. R.; Wheeler, R. M.; Sager, J. C.

2001-01-01

Radish (Raphanus sativus L. cv. Cherriette), lettuce (Lactuca sativa L. cv. Waldmann's Green), and spinach (Spinacea oleracea L. cv. Nordic IV) plants were grown under 660-nm red light-emitting diodes (LEDs) and were compared at equal photosynthetic photon flux (PPF) with either plants grown under cool-white fluorescent lamps (CWF) or red LEDs supplemented with 10% (30 micromoles m-2 s-1) blue light (400-500 nm) from blue fluorescent (BF) lamps. At 21 days after planting (DAP), leaf photosynthetic rates and stomatal conductance were greater for plants grown under CWF light than for those grown under red LEDs, with or without supplemental blue light. At harvest (21 DAP), total dry-weight accumulation was significantly lower for all species tested when grown under red LEDs alone than when grown under CWF light or red LEDs + 10% BF light. Moreover, total dry weight for radish and spinach was significantly lower under red LEDs + 10% BF than under CWF light, suggesting that addition of blue light to the red LEDs was still insufficient for achieving maximal growth for these crops.

The health risks associated with energy efficient fluorescent, LEDs, and artificial lighting

NASA Astrophysics Data System (ADS)

Panahi, Allen

2014-09-01

With the phasing out of incandescent lamps in many countries, the introduction of new LED based light sources and luminaries sometimes raise the question of whether the spectral characteristics of the LED and other energy savings Fluorescent lights including the popular CFLs are suitable to replace the traditional incandescent lamps. These concerns are sometimes raised particularly for radiation emissions in the UV and Blue parts of the spectrum. This paper aims to address such concerns for the common `white light' sources typically used in household and other general lighting used in the work place. Recent studies have shown that women working the night shift have an increased probability of developing breast cancer. We like to report on the findings of many studies done by medical professionals, in particular the recent announcement of AMA in the US and many studies conducted in the UK, as well as the European community to increase public awareness on the long term health risks of the optical and opto-biological effects on the human health caused by artificial lighting.

Identification of Furosemide Photodegradation Products in Water-Acetonitrile Mixture.

PubMed

Katsura, Shinji; Yamada, Nobuo; Nakashima, Atsushi; Shiraishi, Sumihiro; Furuishi, Takayuki; Ueda, Haruhisa

2015-01-01

The aim of this study was to identify the chemical structure of the photodegradation products of furosemide in a water-acetonitrile mixture (1 : 1). Furosemide solution was irradiated with a D65 fluorescent lamp and the products were isolated by preparative HPLC. The fractions were evaporated to dryness in vacuo. The purity of the photodegradation products was measured by HPLC. The purity of products 1, 3, and 4 was greater than 90%, whereas that of product 2 was 13%, therefore, photodegradation product 2 was unstable. We identified photodegradation products 1 and 3 as 4-chloro-5-sulfamoylanthranilic acid and 4-hydroxy-N-furfuryl-5-sulfamoylanthranilic acid, respectively, by LC/MS and NMR. Additionally, we assumed that photodegradation product 4 was methyl 2-((furan-2-ylmethyl)amino)-4-hydroxy-3-(methyleneamino)-5-sulfamoylbenzoate by LC/MS and NMR. This showed that furosemide underwent hydrolysis and substitution, and reacted with the acetonitrile under the light of a D65 fluorescent lamp. We were furthermore able to determine the elution times of the photodegradation products of furosemide by applying the Japanese Pharmacopoeia chromatographic method for related substances to the isolated products.

EDITORIAL: Mercury-free discharges for lighting

NASA Astrophysics Data System (ADS)

Haverlag, M.

2007-07-01

This special Cluster of articles in Journal of Physics D: Applied Physics covers the subject of mercury-free discharges that are being investigated by different light source researchers, as an alternative to existing mercury-containing lamps. The main driving force to move away from mercury-containing discharge light sources is connected to the environmentally unfriendly nature of mercury. After inhalation or direct contact, severe mercury exposure can lead to damage to human brain cells, the kidneys, the liver and the nervous system. For this reason, the use of mercury in products is becoming more and more restricted by different governmental bodies. In the lighting industry, however, many products still make use of mercury, for different reasons. The main reason is that mercury-containing products are, in most cases, more efficient than mercury-free products. For a realistic comparison of the environmental impact, the mercury-contamination due to electricity production must be taken into account, which depends on the type of fuel being used. For an average European fuel-mix, the amount of mercury that is released into the environment is around 29 μg kWh-1. This means that a typical 30 W TL lamp during a lifetime of 20,000 hours will release a total of about 20 mg mercury due to electricity production, which exceeds the total mercury dose in the lamp (more and more of which is being recycled) by a factor of 5-10 for a modern TL lamp. This illustrates that, quite apart from other environmental arguments like increased CO2 production, mercury-free alternatives that use more energy can in fact be detrimental for the total mercury pollution over the lifetime of the lamp. For this reason, the lighting industry has concentrated on lowering the mercury content in lamps as long as no efficient alternatives exist. Nevertheless, new initiatives for HID lamps and fluorescent lamps with more or less equal efficiency are underway, and a number of them are described in this special issue. These initiatives may in time offer realistic alternatives for mercury-containing discharge lamps as the efficiency gap with existing products is getting smaller. At the same time, new applications for radiation sources are becoming more important, and in some of them the presence of mercury has other disadvantages besides the environmental aspects. Since in most cases mercury is used in the form of a saturated vapour, the mercury pressure is dependent on the ambient temperature, which means that mercury-containing lamps often show a slow increase to the steady-state light output or a strongly reduced output in cold environments, which is undesirable in many applications. For this reason also, different options for light sources without mercury are being investigated, and a number of them can be found in this special issue. This collection of papers gives a good overview of the different technologies that are currently being investigated as alternatives to existing lamp technologies, and will surely inspire others to reduce the use of mercury for lighting applications.

A novel monodisperse SiO2@C-dot for the rapid and facile identification of latent fingermarks using self-quenching resistant solid-state fluorescence.

PubMed

Peng, Di; Liu, Xiang; Huang, Mengjun; Wang, Dan; Liu, Renlong

2018-04-24

Solid powder fluorescence shows great potential for application in medicine, biology, and engineering, especially in the identification of latent fingermarks in forensic science. However, conventional developing methods suffer from some drawbacks, such as low contrast, low sensitivity, low selectivity, and high toxicity. To conquer these challenges, novel SiO2@C-dot microspheres were prepared via a facile one-pot hydrothermal method by using citric acid as a carbon source and aminosilane as a nitrogen source. Interestingly, the results showed that the resultant powders possess good monodispersity, high fluorescence emission, and resistance to self-quenching. Additionally, the mechanism for the solid-state fluorescence of SiO2@C-dot compounds was also investigated. More importantly, the fingermarks on various surfaces, including transparent glasses, ceramic tiles, transparent plastics, aluminum alloys, plastic cards, painted woods, artificial leathers, and Chinese paper money, developed by the powders have indicated well-defined papillary ridges under a 365 nm UV lamp. The novel strategy of using monodisperse SiO2@C-dot microspheres as a fluorescent label for developing latent fingermarks showed greater advantages compared to conventional methods, which was also demonstrated using the automatic fingerprint identification system. It is simple, rapid, low-cost, nontoxic, and effective, and is expected to be a promising alternative for the development of latent fingerprints in forensic science.

49 CFR 393.23 - Power supply for lamps.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Power supply for lamps. 393.23 Section 393.23... NECESSARY FOR SAFE OPERATION Lamps, Reflective Devices, and Electrical Wiring § 393.23 Power supply for lamps. All required lamps must be powered by the electrical system of the motor vehicle with the...

49 CFR 393.23 - Power supply for lamps.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 5 2012-10-01 2012-10-01 false Power supply for lamps. 393.23 Section 393.23... NECESSARY FOR SAFE OPERATION Lamps, Reflective Devices, and Electrical Wiring § 393.23 Power supply for lamps. All required lamps must be powered by the electrical system of the motor vehicle with the...

Secondary electron emission characteristics of oxide electrodes in flat electron emission lamp

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chiang, Chang-Lin, E-mail: CLChiang@itri.org.tw; Li, Chia-Hung; Department of Electrophysics, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 300, Taiwan

2016-01-15

The present study concerns with the secondary electron emission coefficient, γ, of the cathode materials used in the newly developed flat electron emission lamp (FEEL) devices, which essentially integrates the concept of using cathode for fluorescent lamp and anode for cathode ray tube (CRT) to obtain uniform planar lighting. Three different cathode materials, namely fluorine-doped tin oxide (FTO), aluminum oxide coated FTO (Al{sub 2}O{sub 3}/FTO) and magnesium oxide coated FTO (MgO/FTO) were prepared to investigate how the variations of γ and working gases influence the performance of FEEL devices, especially in lowering the breakdown voltage and pressure of the workingmore » gases. The results indicate that the MgO/FTO bilayer cathode exhibited a relatively larger effective secondary electron emission coefficient, resulting in significant reduction of breakdown voltage to about 3kV and allowing the device to be operated at the lower pressure to generate the higher lighting efficiency.« less

STITCHER: A web resource for high-throughput design of primers for overlapping PCR applications.

PubMed

O'Halloran, Damien M

2015-06-01

Overlapping PCR is routinely used in a wide number of molecular applications. These include stitching PCR fragments together, generating fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR is also used for genotyping by traditional PCR techniques and in detection experiments using techniques such as loop-mediated isothermal amplification (LAMP). STITCHER is a web tool providing a central resource for researchers conducting all types of overlapping PCR experiments with an intuitive interface for automated primer design that's fast, easy to use, and freely available online (http://ohalloranlab.net/STITCHER.html). STITCHER can handle both single sequence and multi-sequence input, and specific features facilitate numerous other PCR applications, including assembly PCR, adapter PCR, and primer walking. Field PCR, and in particular, LAMP, offers promise as an on site tool for pathogen detection in underdeveloped areas, and STITCHER includes off-target detection features for pathogens commonly targeted using LAMP technology.

Lamp system with conditioned water coolant and diffuse reflector of polytetrafluorethylene(PTFE)

DOEpatents

Zapata, Luis E.; Hackel, Lloyd

1999-01-01

A lamp system with a very soft high-intensity output is provided over a large area by water cooling a long-arc lamp inside a diffuse reflector of polytetrafluorethylene (PTFE) and titanium dioxide (TiO.sub.2) white pigment. The water is kept clean and pure by a one micron particulate filter and an activated charcoal/ultraviolet irradiation system that circulates and de-ionizes and biologically sterilizes the coolant water at all times, even when the long-arc lamp is off.

Lamp system for uniform semiconductor wafer heating

DOEpatents

Zapata, Luis E.; Hackel, Lloyd

2001-01-01

A lamp system with a very soft high-intensity output is provided over a large area by water cooling a long-arc lamp inside a diffuse reflector of polytetrafluorethylene (PTFE) and titanium dioxide (TiO.sub.2) white pigment. The water is kept clean and pure by a one micron particulate filter and an activated charcoal/ultraviolet irradiation system that circulates and de-ionizes and biologically sterilizes the coolant water at all times, even when the long-arc lamp is off.

SearchLight: a freely available web-based quantitative spectral analysis tool (Conference Presentation)

NASA Astrophysics Data System (ADS)

Prabhat, Prashant; Peet, Michael; Erdogan, Turan

2016-03-01

In order to design a fluorescence experiment, typically the spectra of a fluorophore and of a filter set are overlaid on a single graph and the spectral overlap is evaluated intuitively. However, in a typical fluorescence imaging system the fluorophores and optical filters are not the only wavelength dependent variables - even the excitation light sources have been changing. For example, LED Light Engines may have a significantly different spectral response compared to the traditional metal-halide lamps. Therefore, for a more accurate assessment of fluorophore-to-filter-set compatibility, all sources of spectral variation should be taken into account simultaneously. Additionally, intuitive or qualitative evaluation of many spectra does not necessarily provide a realistic assessment of the system performance. "SearchLight" is a freely available web-based spectral plotting and analysis tool that can be used to address the need for accurate, quantitative spectral evaluation of fluorescence measurement systems. This tool is available at: http://searchlight.semrock.com/. Based on a detailed mathematical framework [1], SearchLight calculates signal, noise, and signal-to-noise ratio for multiple combinations of fluorophores, filter sets, light sources and detectors. SearchLight allows for qualitative and quantitative evaluation of the compatibility of filter sets with fluorophores, analysis of bleed-through, identification of optimized spectral edge locations for a set of filters under specific experimental conditions, and guidance regarding labeling protocols in multiplexing imaging assays. Entire SearchLight sessions can be shared with colleagues and collaborators and saved for future reference. [1] Anderson, N., Prabhat, P. and Erdogan, T., Spectral Modeling in Fluorescence Microscopy, http://www.semrock.com (2010).

Investigation of recovery and recycling of rare earth elements from waste fluorescent lamp phosphors

NASA Astrophysics Data System (ADS)

Eduafo, Patrick Max

Characterization techniques and experimental measurements were used to evaluate a process for recycling rare earth elements (REEs) from spent fluorescent lamp phosphors. QEMSCAN analysis revealed that over 60% of the rare earth bearing minerals was less than 10 microm. A representative sample of the as-received feed contained 14.59 wt% total rare earth elements (TREE) and upon sieving to below 75 microm, the grade increased to 19.60 wt% REE with 98.75% recovery. Based on experimental work, a new process for extracting the chief REEs from end of life fluorescent lamps has been developed. The proposed flowsheet employs a three-stage leaching and precipitation process for selective extraction and recovery of the REEs. Hydrochloric acid was used as lixiviant in batch leach experiments on the phosphor powder. The maximum extraction obtained was 100% for both yttrium and europium under the following leaching conditions: 2.5 M HCl, 70°C, 1 hour, 180 g/L and 600 rpm. However, the solubility of cerium, lanthanum and terbium remained low at these conditions. Kinetic data of the leaching of yttrium and europium showed best fit to the logarithmic rate expression of the empirical model of leaching. Activation energy was calculated to be 77.49 kJ/mol for Y and 72.75 kJ/mol for Eu in the temperature range of 298 to 343 K. Precipitation tests demonstrate that at least 50% excess the stoichiometric amount of oxalic acid is needed to recover yttrium and europium efficiently to produce a pure (Y, Eu) mixed oxide. Total recovery of the REEs was achieved even at very low pH or without any base added. Over 99% pure mixed rare earth oxide at 99% recovery has been attained. An economic assessment of the developed process using operating and capital cost have be undertaken and based on the analysis of the three economic scenarios, two are economic and one is non-economic.

Expression of the mRNAs encoding the limbic system-associated membrane protein (LAMP): II. Fetal rat brain.

PubMed

Pimenta, A F; Reinoso, B S; Levitt, P

1996-11-11

The limbic system-associated membrane protein (LAMP) is a 64-68 kDa neuronal surface glycoprotein expressed in cortical and subcortical regions of the limbic system of the adult and developing rat central nervous system (CNS). LAMP is a member of the immunoglobulin superfamily of cell adhesion molecules with three Ig domains and is highly conserved between rat and human. In this study, the temporal and spatial pattern of lamp gene expression during fetal rat development was analyzed by using Northern blot analysis and in situ hybridization. In Northern blot analysis, two lamp mRNA transcripts, 1.6 kb and 8.0 kb, identical in size to those present in the adult rat nervous system, were detected in developing neural tissue. In situ hybridization analysis showed close correlation, though not identity, between the expression of lamp mRNAs and the distribution of LAMP in limbic regions of the developing rat CNS, indicative of a more complex regulation of gene expression than was previously thought to be the case. The expression of lamp mRNAs is first detected on about embryonic day (E) 13. The hybridization signal is not seen in the proliferative ventricular zone at any level of the neuraxis, indicating that lamp is expressed in postmitotic neurons. In the cerebral cortex, lamp mRNAs are expressed in limbic cortical regions, such as the perirhinal cortex, prefrontal cortex, and cingulate cortex. In the hippocampus, the hybridization signal is observed in Ammon's horn by E18. The neostriatum, amygdaloid complex, and most hypothalamic areas express lamp mRNAs from early stages (E13-E14) in a pattern consistent with the onset of neurogenesis. The emerging patterns of lamp expression at the outset are similar to those seen in adult hypothalamus and dorsal thalamus. Although the hybridization signal is observed in some nonlimbic areas, including midbrain and hindbrain structures, intense labeling is evident in more classic limbic regions. The high levels of expression of lamp in limbic regions, beginning in early developmental stages, combined with the results of previous functional in vitro and in vivo studies, support a role for LAMP as a recognition molecule involved in the formation of limbic connections.

Beyond CCT: The spectral index system as a tool for the objective, quantitative characterization of lamps

NASA Astrophysics Data System (ADS)

Galadí-Enríquez, D.

2018-02-01

Correlated color temperature (CCT) is a semi-quantitative system that roughly describes the spectra of lamps. This parameter gives the temperature (measured in kelvins) of the black body that would show the hue more similar to that of the light emitted by the lamp. Modern lamps for indoor and outdoor lighting display many spectral energy distributions, most of them extremely different to those of black bodies, what makes CCT to be far from a perfect descriptor from the physical point of view. The spectral index system presented in this work provides an accurate, objective, quantitative procedure to characterize the spectral properties of lamps, with just a few numbers. The system is an adaptation to lighting technology of the classical procedures of multi-band astronomical photometry with wide and intermediate-band filters. We describe the basic concepts and we apply the system to a representative set of lamps of many kinds. The results lead to interesting, sometimes surprising conclusions. The spectral index system is extremely easy to implement from the spectral data that are routinely measured at laboratories. Thus, including this kind of computations in the standard protocols for the certification of lamps will be really straightforward, and will enrich the technical description of lighting devices.

[Microsecond Pulsed Hollow Cathode Lamp as Enhanced Excitation Source of Hydride Generation Atomic Fluorescence Spectrometry].

PubMed

Zhang, Shuo

2015-09-01

The spectral, electrical and atomic fluorescence characteristics of As, Se, Sb and Pb hollow cathode lamps (HCLs) powered by a laboratory-built high current microsecond pulse (HCMP) power supply were studied, and the feasibility of using HCMP-HCLs as the excitation source of hydride generation atomic fluorescence spectrometry (HG-AFS) was evaluated. Under the HCMP power supply mode, the As, Se, Sb, Pb HCLs can maintain stable glow discharge at frequency of 100~1000 Hz, pulse width of 4.0~20 μs and pulse current up to 4.0 A. Relationship between the intensity of characteristic emission lines and HCMP power supply parameters, such as pulse current, power supply voltage, pulse width and frequency, was studied in detail. Compared with the conventional pulsed (CP) HCLs used in commercial AFS instruments, HCMP-HCLs have a narrower pulse width and much stronger pulse current. Under the optimized HCMP power supply parameters, the intensity of atomic emission lines of As, Se, Sb HCLs had sharp enhancement and that indicated their capacity of being a novel HG-AFS excitation source. However, the attenuation of atomic lines and enhancement of ionic lines negated such feasibility of HCMP-Pb HCL. Then the HG-AFS analytical capability of using the HCMP-As/Se/Sb HCLs excitation source was established and results showed that the HCMP-HCL is a promising excitation source for HG-AFS.

Determining heavy metals in spent compact fluorescent lamps (CFLs) and their waste management challenges: some strategies for improving current conditions.

PubMed

Taghipour, Hassan; Amjad, Zahra; Jafarabadi, Mohamad Asghari; Gholampour, Akbar; Norouz, Prviz

2014-07-01

From environmental viewpoint, the most important advantage of compact fluorescent lamps (CFLs) is reduction of green house gas emissions. But their significant disadvantage is disposal of spent lamps because of containing a few milligrams of toxic metals, especially mercury and lead. For a successful implementation of any waste management plan, availability of sufficient and accurate information on quantities and compositions of the generated waste and current management conditions is a fundamental prerequisite. In this study, CFLs were selected among 20 different brands in Iran. Content of heavy metals including mercury, lead, nickel, arsenic and chromium was determined by inductive coupled plasma (ICP). Two cities, Tehran and Tabriz, were selected for assessing the current waste management condition of CFLs. The study found that waste generation amount of CFLs in the country was about 159.80, 183.82 and 153.75 million per year in 2010, 2011 and 2012, respectively. Waste generation rate of CFLs in Iran was determined to be 2.05 per person in 2012. The average amount of mercury, lead, nickel, arsenic and chromium was 0.417, 2.33, 0.064, 0.056 and 0.012 mg per lamp, respectively. Currently, waste of CFLs is disposed by municipal waste stream in waste landfills. For improving the current conditions, we propose by considering the successful experience of extended producer responsibility (EPR) in other electronic waste management. The EPR program with advanced recycling fee (ARF) is implemented for collecting and then recycling CFLs. For encouraging consumers to take the spent CFLs back at the end of the products' useful life, a proportion of ARF (for example, 50%) can be refunded. On the other hand, the government and Environmental Protection Agency should support and encourage recycling companies of CFLs both technically and financially in the first place. Copyright © 2014 Elsevier Ltd. All rights reserved.

A multimodal spectroscopy system for real-time disease diagnosis

NASA Astrophysics Data System (ADS)

Šćepanović, Obrad R.; Volynskaya, Zoya; Kong, Chae-Ryon; Galindo, Luis H.; Dasari, Ramachandra R.; Feld, Michael S.

2009-04-01

The combination of reflectance, fluorescence, and Raman spectroscopy—termed multimodal spectroscopy (MMS)—provides complementary and depth-sensitive information about tissue composition. As such, MMS is a promising tool for disease diagnosis, particularly in atherosclerosis and breast cancer. We have developed an integrated MMS instrument and optical fiber spectral probe for simultaneous collection of all three modalities in a clinical setting. The MMS instrument multiplexes three excitation sources, a xenon flash lamp (370-740 nm), a nitrogen laser (337 nm), and a diode laser (830 nm), through the MMS probe to excite tissue and collect the spectra. The spectra are recorded on two spectrograph/charge-coupled device modules, one optimized for visible wavelengths (reflectance and fluorescence) and the other for the near-infrared (Raman), and processed to provide diagnostic parameters. We also describe the design and calibration of a unitary MMS optical fiber probe 2 mm in outer diameter, containing a single appropriately filtered excitation fiber and a ring of 15 collection fibers, with separate groups of appropriately filtered fibers for efficiently collecting reflectance, fluorescence, and Raman spectra from the same tissue location. A probe with this excitation/collection geometry has not been used previously to collect reflectance and fluorescence spectra, and thus physical tissue models ("phantoms") are used to characterize the probe's spectroscopic response. This calibration provides probe-specific modeling parameters that enable accurate extraction of spectral parameters. This clinical MMS system has been used recently to analyze artery and breast tissue in vivo and ex vivo.

A Handheld Point-of-Care Genomic Diagnostic System

PubMed Central

Myers, Frank B.; Henrikson, Richard H.; Bone, Jennifer; Lee, Luke P.

2013-01-01

The rapid detection and identification of infectious disease pathogens is a critical need for healthcare in both developed and developing countries. As we gain more insight into the genomic basis of pathogen infectivity and drug resistance, point-of-care nucleic acid testing will likely become an important tool for global health. In this paper, we present an inexpensive, handheld, battery-powered instrument designed to enable pathogen genotyping in the developing world. Our Microfluidic Biomolecular Amplification Reader (µBAR) represents the convergence of molecular biology, microfluidics, optics, and electronics technology. The µBAR is capable of carrying out isothermal nucleic acid amplification assays with real-time fluorescence readout at a fraction of the cost of conventional benchtop thermocyclers. Additionally, the µBAR features cell phone data connectivity and GPS sample geotagging which can enable epidemiological surveying and remote healthcare delivery. The µBAR controls assay temperature through an integrated resistive heater and monitors real-time fluorescence signals from 60 individual reaction chambers using LEDs and phototransistors. Assays are carried out on PDMS disposable microfluidic cartridges which require no external power for sample loading. We characterize the fluorescence detection limits, heater uniformity, and battery life of the instrument. As a proof-of-principle, we demonstrate the detection of the HIV-1 integrase gene with the µBAR using the Loop-Mediated Isothermal Amplification (LAMP) assay. Although we focus on the detection of purified DNA here, LAMP has previously been demonstrated with a range of clinical samples, and our eventual goal is to develop a microfluidic device which includes on-chip sample preparation from raw samples. The µBAR is based entirely around open source hardware and software, and in the accompanying online supplement we present a full set of schematics, bill of materials, PCB layouts, CAD drawings, and source code for the µBAR instrument with the goal of spurring further innovation toward low-cost genetic diagnostics. PMID:23936402

Performance of light sources and radiation sensors under low gravity realized by parabolic airplane flights

NASA Astrophysics Data System (ADS)

Hirai, Hiroaki; Kitaya, Yoshiaki; Hirai, Takehiro

A fundamental study was conducted to establish an experimental system for space farming. Since to ensure optimal light for plant cultivation in space is of grave importance, this study examined the performance of light sources and radiation sensors under microgravity conditions created during the parabolic airplane flight. Three kinds of light sources, a halogen bulb, a fluorescent tube, and blue and red LEDs, and ten models of radiation sensors available in the market were used for the experiment. Surface temperature of the light sources, output signals from the radiation sensors, spectroscopic characteristics were measured at the gravity levels of 0.01, 1.0 and 1.8 G for 20 seconds each during parabolic airplane flights. As a result, the performance of the halogen lamp was affected the most by the gravity level among the three light sources. Under the microgravity conditions which do not raise heat convection, the temperature of the halogen lamp rose and the output of the radiation sensors increased. Spectral distributions of the halogen lamp indicated that peak wavelength appeared the highest at the level of 0.01G, which contributed to the increase in light intensity. In the case of red and blue LEDs, which are promising light sources in space farming, the temperature of both LED chips rose but irradiance from red LED increased and that from blue LED decreased under microgravity conditions due to the different thermal characteristics.

Design and evaluation of excitation light source device for fluorescence endoscope

NASA Astrophysics Data System (ADS)

Lim, Hyun Soo

2009-06-01

This study aims at designing and evaluating light source devices that can stably generate light with various wavelengths in order to make possible PDD using a photosensitizer and diagnosis using auto-fluorescence. The light source was a Xenon lamp and filter wheel, composed of an optical output control through Iris and filters with several wavelength bands. It also makes the inducement of auto-fluorescence possible because it is designed to generate a wavelength band of 380-420nm, 430-480nm, and 480-560nm. The transmission part of the light source was developed to enhance the efficiency of light transmission. To evaluate this light source, the characteristics of light output and wavelength band were verified. To validate the capability of this device as PDD, the detection of auto-fluorescence using mouse models was performed.

Impact assessment of energy-efficient lighting in patients with lupus erythematosus: a pilot study.

PubMed

Fenton, L; Dawe, R; Ibbotson, S; Ferguson, J; Silburn, S; Moseley, H

2014-03-01

Patients with lupus erythematosus (LE) are often abnormally photosensitive. Ultraviolet (UV) exposure can not only induce cutaneous lesions but may also contribute to systemic flares and disease progression. Various forms of energy-efficient lighting have been shown to emit UV radiation. To determine the effects of these emissions on individuals with LE. This assessment investigated cutaneous responses to repeated exposures from three types of lighting: compact fluorescent lamp (CFL), light-emitting diode (LED) and energy-efficient halogen (EEH). The subjects were 15 patients with LE and a control group of five healthy volunteers. No cutaneous LE lesions were induced by any of the light sources. Delayed skin erythema was induced at the site of CFL irradiation in six of the 15 patients with LE and two of the five healthy subjects. Erythema was increased in severity and more persistent in patients with LE. One patient with LE produced a positive delayed erythema to the EEH. A single patient with LE produced immediate abnormal erythemal responses to the CFL, LED and EEH. Further investigation revealed that this patient also had solar urticaria. All other subjects had negative responses to LED exposure. Compact fluorescent lamps emit UV that can induce skin erythema in both individuals with LE and healthy individuals when situated in close proximity. However, this occurs to a greater extent and is more persistent in patients with LE. EEHs emit UVA that can induce erythema in patients with LE. LEDs provide a safer alternative light source without risk of UV exposure. © 2013 British Association of Dermatologists.

Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting

NASA Technical Reports Server (NTRS)

Goins, G. D.; Yorio, N. C.; Sanwo, M. M.; Brown, C. S.; Sager, J. C. (Principal Investigator)

1997-01-01

Red light-emitting diodes (LEDs) are a potential light source for growing plants in spaceflight systems because of their safety, small mass and volume, wavelength specificity, and longevity. Despite these attractive features, red LEDs must satisfy requirements for plant photosynthesis and photomorphogenesis for successful growth and seed yield. To determine the influence of gallium aluminium arsenide (GaAlAs) red LEDs on wheat photomorphogenesis, photosynthesis, and seed yield, wheat (Triticum aestivum L., cv. 'USU-Super Dwarf') plants were grown under red LEDs and compared to plants grown under daylight fluorescent (white) lamps and red LEDs supplemented with either 1% or 10% blue light from blue fluorescent (BF) lamps. Compared to white light-grown plants, wheat grown under red LEDs alone demonstrated less main culm development during vegetative growth through preanthesis, while showing a longer flag leaf at 40 DAP and greater main culm length at final harvest (70 DAP). As supplemental BF light was increased with red LEDs, shoot dry matter and net leaf photosynthesis rate increased. At final harvest, wheat grown under red LEDs alone displayed fewer subtillers and a lower seed yield compared to plants grown under white light. Wheat grown under red LEDs+10% BF light had comparable shoot dry matter accumulation and seed yield relative to wheat grown under white light. These results indicate that wheat can complete its life cycle under red LEDs alone, but larger plants and greater amounts of seed are produced in the presence of red LEDs supplemented with a quantity of blue light.

In vivo imaging of small animals with optical tomography and near-infrared fluorescent probes

NASA Astrophysics Data System (ADS)

Palmer, Matthew R.; Shibata, Yasushi; Kruskal, Jonathan B.; Lenkinski, Robert E.

2002-06-01

A developmental optical tomography has been designed for imaging small animals in vivo using near IR fluorophores. The system employs epi-illumination via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high- resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180 degrees rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions form ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.

75 FR 14287 - Energy Conservation Program: Test Procedures for Fluorescent Lamp Ballasts

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-24

... Government Appropriations Act, 2001 J. Executive Order 13211 K. Executive Order 12630 L. Section 32 of the... and Background Title III of the Energy Policy and Conservation Act (42 U.S.C. 6291 et seq.; EPCA or... (42 U.S.C. 6291-6309) establishes the ``Energy Conservation Program for Consumer Products Other Than...

76 FR 70547 - Energy Conservation Program: Energy Conservation Standards for Fluorescent Lamp Ballasts

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-14

... Rejected. C. Review Under the Paperwork Reduction Act D. Review Under the National Environmental Policy Act... Appropriations Act, 1999 I. Review Under Executive Order 12630 J. Review Under the Treasury and General... (EPCA or the Act), Public Law 94-163 (42 U.S.C. 6291-6309, as codified), established the Energy...

Tree seedling response to LED spectra: Implications for forest restoration

Treesearch

Antonio Montagnoli; R. Kasten Dumroese; Mattia Terzaghi; Jeremiah R. Pinto; Nicoletta Fulgaro; Gabriella Stefania Scippa; Donato Chiatante

2018-01-01

We found that different spectra, provided by light-emitting diodes or a fluorescent lamp, caused different photomorphological responses depending on tree seedling type (coniferous or broad-leaved), species, seedling development stage, and seedling fraction (shoot or root). For two conifers (Picea abies and Pinus sylvestris) soon after germination (≤40 days), more...

78 FR 16709 - Certain Cold Cathode Fluorescent Lamp (“CCFL”) Inverter Circuits and Products Containing Same...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-18

.... International Trade Commission, 500 E Street SW., Washington, DC 20436, telephone (202) 205-2737. The public... the Office of the Secretary, U.S. International Trade Commission, 500 E Street SW., Washington, DC... the Commission. Lisa R. Barton, Acting Secretary to the Commission. [FR Doc. 2013-06166 Filed 3-15-13...

78 FR 46368 - Certain Dimmable Compact Fluorescent Lamps and Products Containing Same; Termination of an...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-31

...; Zhejiang Qiang Ling Electronic Co. Ltd. of Zhenjiang, China (collectively, ``TCP''); U Lighting America Inc... of the '318 patent have been asserted against TCP. On February 27, 2013, the ALJ issued his final... domestic industry requirement of section 337. The ALJ also found that respondent TCP's accused products do...

78 FR 36574 - Certain Dimmable Compact Fluorescent Lamps and Products Containing Same; Termination as to Three...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-18

... domestic industry requirement. The Commission has also determined to review the ALJ's claim construction of.... 1337, by reason of the infringement of certain claims of United States Patent Nos. 5,434,480 (``the... (``ULA''); and Golden U Lighting Manufacturing (Shenzhen) of Shenzhen, China (``Golden U''). Claim 9 of...

Hybrid phosphorescence and fluorescence native spectroscopy for breast cancer detection.

PubMed

Alimova, Alexandra; Katz, A; Sriramoju, Vidyasagar; Budansky, Yuri; Bykov, Alexei A; Zeylikovich, Roman; Alfano, R R

2007-01-01

Fluorescence and phosphorescence measurements are performed on normal and malignant ex vivo human breast tissues using UV LED and xenon lamp excitation. Tryptophan (trp) phosphorescence intensity is higher in both normal glandular and adipose tissue when compared to malignant tissue. An algorithm based on the ratio of trp fluorescence intensity at 345 nm to phosphorescence intensity at 500 nm is successfully used to separate normal from malignant tissue types. Normal specimens consistently exhibited a low I(345)I(500) ratio (<10), while for malignant specimens, the I(345)I(500) ratio is consistently high (>15). The ratio analysis correlates well with histopathology. Intensity ratio maps with a spatial resolution of 0.5 mm are generated in which local regions of malignancy could be identified.

Programmable LED-based integrating sphere light source for wide-field fluorescence microscopy.

PubMed

Rehman, Aziz Ul; Anwer, Ayad G; Goldys, Ewa M

2017-12-01

Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365-490nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells. Copyright © 2017 Elsevier B.V. All rights reserved.

Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip from Whole Blood

PubMed Central

Damhorst, Gregory L.; Duarte-Guevara, Carlos; Chen, Weili; Ghonge, Tanmay; Cunningham, Brian T.; Bashir, Rashid

2015-01-01

Viral load measurements are an essential tool for the long-term clinical care of hum an immunodeficiency virus (HIV)-positive individuals. The gold standards in viral load instrumentation, however, are still too limited by their size, cost, and sophisticated operation for these measurements to be ubiquitous in remote settings with poor healthcare infrastructure, including parts of the world that are disproportionately affected by HIV infection. The challenge of developing a point-of-care platform capable of making viral load more accessible has been frequently approached but no solution has yet emerged that meets the practical requirements of low cost, portability, and ease-of-use. In this paper, we perform reverse-transcription loop-mediated isothermal amplification (RT-LAMP) on minimally processed HIV-spiked whole blood samples with a microfluidic and silicon microchip platform, and perform fluorescence measurements with a consumer smartphone. Our integrated assay shows amplification from as few as three viruses in a ~ 60 nL RT-LAMP droplet, corresponding to a whole blood concentration of 670 viruses per µL of whole blood. The technology contains greater power in a digital RT-LAMP approach that could be scaled up for the determination of viral load from a finger prick of blood in the clinical care of HIV-positive individuals. We demonstrate that all aspects of this viral load approach, from a drop of blood to imaging the RT-LAMP reaction, are compatible with lab-on-a-chip components and mobile instrumentation. PMID:26705482

Quenching of Unincorporated Amplification Signal Reporters in Reverse-Transcription Loop-Mediated Isothermal Amplification Enabling Bright, Single-Step, Closed-Tube, and Multiplexed Detection of RNA Viruses.

PubMed

Ball, Cameron S; Light, Yooli K; Koh, Chung-Yan; Wheeler, Sarah S; Coffey, Lark L; Meagher, Robert J

2016-04-05

Reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) has frequently been proposed as an enabling technology for simplified diagnostic tests for RNA viruses. However, common detection techniques used for LAMP and RT-LAMP have drawbacks, including poor discrimination capability, inability to multiplex targets, high rates of false positives, and (in some cases) the requirement of opening reaction tubes postamplification. Here, we present a simple technique that allows closed-tube, target-specific detection, based on inclusion of a dye-labeled primer that is incorporated into a target-specific amplicon if the target is present. A short, complementary quencher hybridizes to unincorporated primer upon cooling down at the end of the reaction, thereby quenching fluorescence of any unincorporated primer. Our technique, which we term QUASR (for quenching of unincorporated amplification signal reporters, read "quasar"), does not significantly reduce the amplification efficiency or sensitivity of RT-LAMP. Equipped with a simple LED excitation source and a colored plastic gel filter, the naked eye or a camera can easily discriminate between positive and negative QUASR reactions, which produce a difference in signal of approximately 10:1 without background subtraction. We demonstrate that QUASR detection is compatible with complex sample matrices such as human blood, using a novel LAMP primer set for bacteriophage MS2 (a model RNA virus particle). Furthermore, we demonstrate single-tube duplex detection of West Nile virus (WNV) and chikungunya virus (CHIKV) RNA.

Rapid and Sensitive Detection of Shigella spp. and Salmonella spp. by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique

PubMed Central

Wang, Yi; Wang, Yan; Luo, Lijuan; Liu, Dongxin; Luo, Xia; Xu, Yanmei; Hu, Shoukui; Niu, Lina; Xu, Jianguo; Ye, Changyun

2015-01-01

Shigella and Salmonella are frequently isolated from various food samples and can cause human gastroenteritis. Here, a novel multiple endonuclease restriction real-time loop-mediated isothermal amplification technology (MERT-LAMP) were successfully established and validated for simultaneous detection of Shigella strains and Salmonella strains in only a single reaction. Two sets of MERT-LAMP primers for 2 kinds of pathogens were designed from ipaH gene of Shigella spp. and invA gene of Salmonella spp., respectively. Under the constant condition at 63°C, the positive results were yielded in as short as 12 min with the genomic DNA extracted from the 19 Shigella strains and 14 Salmonella strains, and the target pathogens present in a sample could be simultaneously identified based on distinct fluorescence curves in real-time format. Accordingly, the multiplex detection assay significantly reduced effort, materials and reagents used, and amplification and differentiation were conducted at the same time, obviating the use of postdetection procedures. The analytical sensitivity of MERT-LAMP was found to be 62.5 and 125 fg DNA/reaction with genomic templates of Shigella strains and Salmonella strains, which was consist with normal LAMP assay, and at least 10- and 100-fold more sensitive than that of qPCR and conventional PCR approaches. The limit of detection of MERT-LAMP for Shigella strains and Salmonella strains detection in artificially contaminated milk samples was 5.8 and 6.4 CFU per vessel. In conclusion, the MERT-LAMP methodology described here demonstrated a potential and valuable means for simultaneous screening of Shigella and Salmonella in a wide variety of samples. PMID:26697000

A laser-spectroscopy complex for fluorescent diagnostics and photodynamic therapy of age-related macula degeneration

NASA Astrophysics Data System (ADS)

Shevchik, S. A.; Meerovich, Gennadii A.; Budzinskaya, M. V.; Ermakova, N. A.; Kharnas, Sergey S.; Loschenov, Victor B.

2004-06-01

A laser-spectroscopy complex was developed for fluorescent diagnostics and photodynamic therapy of age related macula degeneration using the Russian photosensitizer Photosense. The complex is based on slit lamp which was additionally equipped with an optical adapter, and the video adapter allows to combine the procedure of photodynamic therapy and the control of its carrying in the frame work of one procedure. The sensitivity and spatial resolution of the complex were investigated using a special test object. The availability of the developed complex and Photosense itself was examined on experimental animals.

Hyperfine structure measurements of neutral vanadium by laser-induced fluorescence spectroscopy in the wavelength range from 750 nm to 860 nm

NASA Astrophysics Data System (ADS)

Başar, Gü.; Güzelçimen, F.; Öztürk, I. K.; Er, A.; Bingöl, D.; Kröger, S.; Başar, Gö.

2017-11-01

The hyperfine structure of 57 spectral lines of neutral vanadium has been investigated using a hollow cathode lamp by laser-induced fluorescence spectroscopy in the wavelength range from 750 nm to 860 nm. New magnetic dipole hyperfine structure constants A have been determined for 14 atomic energy levels and new electric quadrupole hyperfine structure constants B for two levels. Additionally previously published hyperfine structure constants A of 56 levels have been measured again. In five cases, the old A values have been rejected and replaced by improved values.

[Development of X-ray excited fluorescence spectrometer].

PubMed

Ni, Chen; Gu, Mu; Di, Wang; Cao, Dun-Hua; Liu, Xiao-Lin; Huang, Shi-Ming

2009-08-01

An X-ray excited fluorescence spectrometer was developed with an X-ray tube and a spectrometer. The X-ray tube, spectrometer, autocontrol method and data processing selected were roundly evaluated. The wavelength and detecting efficiency of the apparatus were calibrated with the mercury and tungsten bromine standard lamps, and the X-ray excited emission spectra of BaF2, Cs I (Tl) crystals were measured. The results indicate that the apparatus has advantages of good wavelength resolution, high stability, easy to operation and good radioprotection. It is a wery effective tool for exploration of new scintillation materials.

Higher production of C-phycocyanin by nitrogen-free (diazotrophic) cultivation of Nostoc sp. NK and simplified extraction by dark-cold shock.

PubMed

Lee, Na Kyeong; Oh, Hee-Mock; Kim, Hee-Sik; Ahn, Chi-Yong

2017-03-01

Nostoc sp. NK (KCTC 12772BP) was isolated and cultivated in a BG11 medium and a nitrate-free BG11 medium (BG11 0 ). To enhance C-phycocyanin (C-PC) content in the cells, different fluorescent lamps (white, plant, and red) were used as light sources for complementary chromatic adaptation (CCA). The maximum biomass productivity was 0.42g/L/d and 0.32g/L/d under BG11 and BG11 0 conditions, respectively. The maximum C-PC contents were 8.4% (w/w) under white lamps, 13.6% (w/w) under plant lamps, and 18% (w/w) under BG11 0 and the red light condition. The maximum C-PC productivity was 57.4mg/L/d in BG11 0 under the red lamp condition. These results indicate that a higher C-PC content could be obtained under a diazotrophic condition and a CCA reaction. The C-PC could be released naturally from cells without any extraction processes, when Nostoc sp. NK was cultivated in the BG11 0 medium with CO 2 aeration and put in dark conditions at 5°C. Copyright © 2016 Elsevier Ltd. All rights reserved.

Development of mRNA-based body fluid identification using reverse transcription loop-mediated isothermal amplification.

PubMed

Satoh, Tetsuya; Kouroki, Seiya; Ogawa, Keita; Tanaka, Yorika; Matsumura, Kazutoshi; Iwase, Susumu

2018-04-25

Identifying body fluids from forensic samples can provide valuable evidence for criminal investigations. Messenger RNA (mRNA)-based body fluid identification was recently developed, and highly sensitive parallel identification using reverse transcription polymerase chain reaction (RT-PCR) has been described. In this study, we developed reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a simple, rapid assay for identifying three common forensic body fluids, namely blood, semen, and saliva, and evaluated its specificity and sensitivity. Hemoglobin beta (HBB), transglutaminase 4 (TGM4), and statherin (STATH) were selected as marker genes for blood, semen, and saliva, respectively. RT-LAMP could be performed in a single step including both reverse transcription and DNA amplification under an isothermal condition within 60 min, and detection could be conveniently performed via visual fluorescence. Marker-specific amplification was performed in each assay, and no cross-reaction was observed among five representative forensically relevant body fluids. The detection limits of the assays were 0.3 nL, 30 nL, and 0.3 μL for blood, semen, and saliva, respectively, and their sensitivities were comparable with those of RT-PCR. Furthermore, RT-LAMP assays were applicable to forensic casework samples. It is considered that RT-LAMP is useful for body fluid identification.

Design of a Borescope for Extravehicular Non-Destructive Applications

NASA Technical Reports Server (NTRS)

Bachnak, Rafic

2003-01-01

Anomalies such as corrosion, structural damage, misalignment, cracking, stress fiactures, pitting, or wear can be detected and monitored by the aid of a borescope. A borescope requires a source of light for proper operation. Today s current lighting technology market consists of incandescent lamps, fluorescent lamps and other types of electric arc and electric discharge vapor lamp. Recent advances in LED technology have made LEDs viable for a number of applications, including vehicle stoplights, traffic lights, machine-vision-inspection, illumination, and street signs. LEDs promise significant reduction in power consumption compared to other sources of light. This project focused on comparing images taken by the Olympus IPLEX, using two different light sources. One of the sources is the 50-W internal metal halide lamp and the other is a 1 W LED placed at the tip of the insertion tube. Images acquired using these two light sources were quantitatively compared using their histogram, intensity profile along a line segment, and edge detection. Also, images were qualitatively compared using image registration and transformation [l]. The gray-level histogram, edge detection, image profile and image registration do not offer conclusive results. The LED light source, however, produces good images for visual inspection by an operator. Analysis using pattern recognition using Eigenfaces and Gaussian Pyramid in face recognition may be more useful.

49 CFR 234.221 - Lamp voltage.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Lamp voltage. 234.221 Section 234.221... EMERGENCY NOTIFICATION SYSTEMS Maintenance, Inspection, and Testing Maintenance Standards § 234.221 Lamp voltage. The voltage at each lamp shall be maintained at not less than 85 percent of the prescribed rating...

49 CFR 234.221 - Lamp voltage.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Lamp voltage. 234.221 Section 234.221... EMERGENCY NOTIFICATION SYSTEMS Maintenance, Inspection, and Testing Maintenance Standards § 234.221 Lamp voltage. The voltage at each lamp shall be maintained at not less than 85 percent of the prescribed rating...

49 CFR 234.221 - Lamp voltage.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Lamp voltage. 234.221 Section 234.221... EMERGENCY NOTIFICATION SYSTEMS Maintenance, Inspection, and Testing Maintenance Standards § 234.221 Lamp voltage. The voltage at each lamp shall be maintained at not less than 85 percent of the prescribed rating...

Synthesis and fluorescence properties of some difluoroboron β-diketonate complexes and composite containing PMMA

NASA Astrophysics Data System (ADS)

Xing, Dongye; Hou, Yanjun; Niu, Haijun

2018-03-01

A series of difluoroboron β-diketonate complexes, containing the indon-β-diketonate ligand carrying methyl or methoxyl substituents was synthesized. The crystal structures of the complexes were confirmed by single crystal X-ray diffraction studies. The fluorescence properties of compounds were studied in solution state, solid state and on PMMA polymer matrix. The photophysical data of compounds 2a-2d exhibited strong fluorescence and photostability under the ultraviolet light (Hg lamp). The complex 2b showed higher fluorescence intensity in solution state as compared to other complexes of the series. The complexes 2c and 2d showed higher fluorescence intensity in the solid state, which are ascribed to the stronger π-π interactions between ligands in the solid state. The introduction of methoxyl or methyl groups on the benzene rings enhanced the absorption intensity, emission intensity, quantum yields and fluorescence lifetimes due to their electron-donating nature. Furthermore, the complex 2b was doped into the PMMA to produce hybrid materials, where the PMMA matrix acted as sensitizer for the central boron ion to enhance the fluorescence emission intensity and quantum yields.

CALiPER Report 22.1: Photoelectric Performance of LED MR16 Lamps

DOE Office of Scientific and Technical Information (OSTI.GOV)

None, None

This report looks at the photoelectric performance of the same set of lamps assessed in Report 22, using commercially available transformers and dimmers as well as laboratory power supplies providing either AC or DC. The investigation explores several issues related to the testing and use of MR16 lamps in lighting systems and examines the range of performance that is possible for a given lamp model, based on the system to which it is connected.

Integrated smartphone imaging of quantum dot photoluminescence and Förster resonance energy transfer

NASA Astrophysics Data System (ADS)

Petryayeva, Eleonora; Algar, W. Russ

2015-06-01

Smartphones and other mobile devices are emerging as promising analytical platforms for point-of-care diagnostics, particularly when combined with nanotechnology. For example, we have shown that the optical properties of semiconductor quantum dots (QDs) are well suited to photoluminescence (PL) detection with a smartphone camera. However, this previous work has utilized an external excitation source for interrogation of QD PL. In this proceeding, we demonstrate that the white-light LED photographic flashes built into smartphones can be optically filtered to yield blue light suitable for excitation of QD PL. Measurements were made by recording video with filtered flash illumination and averaging the frames of the video to obtain images with good signal-to-background ratios. These images permitted detection of green-emitting and red-emitting QDs at levels comparable to those possible with excitation using an external long-wave UV lamp. The optical properties of QDs proved to be uniquely suited to smartphone PL imaging, exhibiting emission that was 1-2 orders magnitude brighter than that of common fluorescent dyes under the same conditions. Excitation with the smartphone flash was also suitable for imaging of FRET between green-emitting QD donors and Alexa Fluor 555 (A555) fluorescent dye acceptors. No significant difference in FRET imaging capability was observed between excitation with the smartphone flash and a long-wave UV lamp. Although the smartphone flash did have some disadvantages compared to an external UV lamp, these disadvantages are potentially offset by the benefit of having excitation and detection integrated into the smartphone.

Performance of 'energy efficient' compact fluorescent lamps.

PubMed

Yuen, Gloria S-C; Sproul, Alistair B; Dain, Stephen J

2010-03-01

Compact fluorescent lamps (CFLs) have been heralded as highly energy efficient replacements for incandescent light globes, however, there is some public dissatisfaction with the light output and colour of CFLs. Independent examination of the claims made has not been made. Compliance with the interim Australian/New Zealand Standard has not been established by any independent authority. While the total light output (luminous flux) may meet certain standards, luminous intensity distributions of some designs do differ significantly from the incandescent sources that they are intended to replace. Luminous intensity distribution, luminous flux and spectral energy distribution of CFLs claimed to be equivalent to 75 W incandescent globes and 75 W incandescent globes (pearl and clear) were measured. Luminous flux, luminous efficacy, colour rendering index, correlated colour temperature, wattage and power factor were then calculated and compared with claims made by manufacturers and requirements of the standards. The sources generally complied with the requirements for luminous flux, luminous efficacy, colour rendering index and correlated colour temperature. The claim of 75 W equivalence, which is not regulated in Australia and New Zealand, is justified less than half the time. Luminous intensity distributions of biaxial CFLs are distinctly different from the incandescent lamps they purport to replace. CFLs generally comply with the standards set. The basis on which equivalent wattages are claimed needs to be included in the Australian and New Zealand standard because this is the measure most likely to be relied on by the public. Due to the differences in luminous intensity distribution, CFLs may not necessarily be a direct replacement for incandescent sources without some consideration.

Long term spectral irradiance measurements of a 1000-watt xenon arc lamp

NASA Technical Reports Server (NTRS)

Schneider, W. E.

1974-01-01

Spectral irradiance measurements over the range of 200 to 1060 nm were made on a 1000-watt xenon arc lamp over a period of 1500 hours. Four sets of measurements were made after periods of 70, 525, 1000, and 1500 hours of operation. The lamp (Hanovia Compact Xenon Arc Lamp) was mounted in the NASA Solar Irradiation System. When used in the System, the lamp is used as the radiating source for six test stations. Measurements were made of both the longterm stability (or variation of spectral irradiance as a function of time) and the actual spectral irradiance incident on the test specimen.

A Survey of Plasmas and Their Applications

NASA Technical Reports Server (NTRS)

Eastman, Timothy E.; Grabbe, C. (Editor)

2006-01-01

Plasmas are everywhere and relevant to everyone. We bath in a sea of photons, quanta of electromagnetic radiation, whose sources (natural and artificial) are dominantly plasma-based (stars, fluorescent lights, arc lamps.. .). Plasma surface modification and materials processing contribute increasingly to a wide array of modern artifacts; e.g., tiny plasma discharge elements constitute the pixel arrays of plasma televisions and plasma processing provides roughly one-third of the steps to produce semiconductors, essential elements of our networking and computing infrastructure. Finally, plasmas are central to many cutting edge technologies with high potential (compact high-energy particle accelerators; plasma-enhanced waste processors; high tolerance surface preparation and multifuel preprocessors for transportation systems; fusion for energy production).

(E)-5-[2-(methoxycarbonyl)ethenyl]cytidine as a chemical actinometer for germicidal UV radiation.

PubMed

Shen, Chengyue; Fang, Shiyue; Bergstrom, Donald E; Blatchley, Ernest R

2005-05-15

(E)-5-[2-(Methoxycarbonyl)ethenyl]cytidine (S) was examined for use as a chemical actinometer for germicidal UV radiation. Its photoproduct, 3-beta-D-ribofuranosyl-2,7-dioxopyrido[2,3-d]pyrimidine (P), is strongly fluorescent with excitation and emission maxima at 330 and 385 nm, respectively. Experiments were conducted to characterize the dynamic behavior of aqueous solutions of S and P when subjected to UV radiation. UV sources used for these experiments included a low-pressure mercury lamp, a XeBr excimer lamp, and a KrCI excimer lamp; all three sources were mounted in collimating devices to provide incident beams that could be easily and accurately characterized by radiometry. These three sources each yielded essentially monochromatic outputwith characteristic wavelengths of 254, 282, and 222 nm, respectively. At practical concentrations, it was found that the absorbance of the actinometer solution was neither high enough to make the actinometer solutions optically opaque nor low enough to be optically transparent to UV. In addition, the photoproduct displayed a molar absorption coefficient that was similar in magnitude to that of the parent compound, thereby resulting in competitive absorption of UV energy between Sand Pduring irradiation. For purposes of evaluation of the results of irradiation, a mathematical model was developed to accountforthe nonideal optical characteristics of the system. The model is based on a description of local photochemical kinetics; predictions of overall reactor performance were developed by spatial and temporal integration of model results. The model was used to analyze the dynamic behavior of actinometer solutions during UV irradiation and to estimate the quantum yield for photoproduction of Pfrom S. This modeling approach is potentially applicable to other photochemical processes in which multiple compounds are present that absorb photoactive radiation; however, general application of this modeling approach to photochemical reactor systems will require inclusion of othertermsto describe relevanttransport behavior within the system.

Application of Wireless Intelligent Control System for HPS Lamps and LEDs Combined Illumination in Road Tunnel

PubMed Central

Lai, Jinxing; Qiu, Junling; Chen, Jianxun; Wang, Yaqiong; Fan, Haobo

2014-01-01

Because of the particularity of the environment in the tunnel, the rational tunnel illumination system should be developed, so as to optimize the tunnel environment. Considering the high cost of traditional tunnel illumination system with high-pressure sodium (HPS) lamps as well as the effect of a single light source on tunnel entrance, the energy-saving illumination system with HPS lamps and LEDs combined illumination in road tunnel, which could make full use of these two kinds of lamps, was proposed. The wireless intelligent control system based on HPS lamps and LEDs combined illumination and microcontrol unit (MCU) Si1000 wireless communication technology was designed. And the remote monitoring, wireless communication, and PWM dimming module of this system were designed emphatically. Intensity detector and vehicle flow detector can be configured in wireless intelligent control system, which gather the information to the master control unit, and then the information is sent to the monitoring center through the Ethernet. The control strategies are got by the monitoring center according to the calculated results, and the control unit wirelessly sends parameters to lamps, which adjust the luminance of each segment of the tunnel and realize the wireless intelligent control of combined illumination in road tunnel. PMID:25587266

46 CFR 167.45-1 - Steam, carbon dioxide, Halon 1301, and clean agent fire extinguishing systems.

Code of Federal Regulations, 2014 CFR

2014-10-01

... holds, paint lockers, and similar spaces. However, although existing steam smothering systems may be... to each cargo-oil deep tank, lamp locker, oil room, and like compartments, which lamp locker, oil... lamp lockers, oil rooms, and like compartments may be taken from the nearest steam supply line...

46 CFR 167.45-1 - Steam, carbon dioxide, Halon 1301, and clean agent fire extinguishing systems.

Code of Federal Regulations, 2012 CFR

2012-10-01

... holds, paint lockers, and similar spaces. However, although existing steam smothering systems may be... to each cargo-oil deep tank, lamp locker, oil room, and like compartments, which lamp locker, oil... lamp lockers, oil rooms, and like compartments may be taken from the nearest steam supply line...

46 CFR 167.45-1 - Steam, carbon dioxide, Halon 1301, and clean agent fire extinguishing systems.

Code of Federal Regulations, 2013 CFR

2013-10-01

... holds, paint lockers, and similar spaces. However, although existing steam smothering systems may be... to each cargo-oil deep tank, lamp locker, oil room, and like compartments, which lamp locker, oil... lamp lockers, oil rooms, and like compartments may be taken from the nearest steam supply line...

Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy

NASA Astrophysics Data System (ADS)

Li, Hewang; Yu, Peiying; Sun, Yuansheng; Felder, Robin A.; Periasamy, Ammasi; Jose, Pedro A.

2010-09-01

The dynamic regulation of the cellular trafficking of human angiotensin (Ang) type 1 receptor (AT1R) is not well understood. Therefore, we investigated the cellular trafficking of AT1R-enhanced green fluorescent protein (EGFP) (AT1R-EGFP) heterologously expressed in HEK293 cells by determining the change in donor lifetime (AT1R-EGFP) in the presence or absence of acceptor(s) using fluorescence lifetime imaging-fluorescence resonance energy transfer (FRET) microscopy. The average lifetime of AT1R-EGFP in our donor-alone samples was ~2.33 ns. The basal state lifetime was shortened slightly in the presence of Rab5 (2.01+/-0.10 ns) or Rab7 (2.11+/-0.11 ns) labeled with Alexa 555, as the acceptor fluorophore. A 5-min Ang II treatment markedly shortened the lifetime of AT1R-EGFP in the presence of Rab5-Alexa 555 (1.78+/-0.31 ns) but was affected minimally in the presence of Rab7-Alexa 555 (2.09+/-0.37 ns). A 30-min Ang II treatment further decreased the AT1R-EGFP lifetime in the presence of both Rab5- and Rab7-Alexa 555. Latrunculin A but not nocodazole pretreatment blocked the ability of Ang II to shorten the AT1R-EGFP lifetime. The occurrence of FRET between AT1R-EGFP (donor) and LAMP1-Alexa 555 (acceptor) with Ang II stimulation was impaired by photobleaching the acceptor. These studies demonstrate that Ang II-induced AT1R lysosomal degradation through its association with LAMP1 is regulated by Rab5/7 via mechanisms that are dependent on intact actin cytoskeletons.

A New Low Cost Wide-Field Illumination Method for Photooxidation of Intracellular Fluorescent Markers

PubMed Central

da Silva Filho, Manoel; Santos, Daniel Valle Vasconcelos; Costa, Kauê Machado

2013-01-01

Analyzing cell morphology is crucial in the fields of cell biology and neuroscience. One of the main methods for evaluating cell morphology is by using intracellular fluorescent markers, including various commercially available dyes and genetically encoded fluorescent proteins. These markers can be used as free radical sources in photooxidation reactions, which in the presence of diaminobenzidine (DAB) forms an opaque and electron-dense precipitate that remains localized within the cellular and organelle membranes. This method confers many methodological advantages for the investigator, including absence of photo-bleaching, high visual contrast and the possibility of correlating optical imaging with electron microscopy. However, current photooxidation techniques require the continuous use of fluorescent or confocal microscopes, which wastes valuable mercury lamp lifetime and limits the conversion process to a few cells at a time. We developed a low cost optical apparatus for performing photooxidation reactions and propose a new procedure that solves these methodological restrictions. Our “photooxidizer” consists of a high power light emitting diode (LED) associated with a custom aluminum and acrylic case and a microchip-controlled current source. We demonstrate the efficacy of our method by converting intracellular DiI in samples of developing rat neocortex and post-mortem human retina. DiI crystals were inserted in the tissue and allowed to diffuse for 20 days. The samples were then processed with the new photooxidation technique and analyzed under optical microscopy. The results show that our protocols can unveil the fine morphology of neurons in detail. Cellular structures such as axons, dendrites and spine-like appendages were well defined. In addition to its low cost, simplicity and reliability, our method precludes the use of microscope lamps for photooxidation and allows the processing of many labeled cells simultaneously in relatively large tissue samples with high efficacy. PMID:23441199

Towards the implementation of a spectral database for the detection of biological warfare agents

NASA Astrophysics Data System (ADS)

Carestia, M.; Pizzoferrato, R.; Gelfusa, M.; Cenciarelli, O.; D'Amico, F.; Malizia, A.; Scarpellini, D.; Murari, A.; Vega, J.; Gaudio, P.

2014-10-01

The deliberate use of biological warfare agents (BWA) and other pathogens can jeopardize the safety of population, fauna and flora, and represents a concrete concern from the military and civil perspective. At present, the only commercially available tools for fast warning of a biological attack can perform point detection and require active or passive sampling collection. The development of a stand-off detection system would be extremely valuable to minimize the risk and the possible consequences of the release of biological aerosols in the atmosphere. Biological samples can be analyzed by means of several optical techniques, covering a broad region of the electromagnetic spectrum. Strong evidence proved that the informative content of fluorescence spectra could provide good preliminary discrimination among those agents and it can also be obtained through stand-off measurements. Such a system necessitates a database and a mathematical method for the discrimination of the spectral signatures. In this work, we collected fluorescence emission spectra of the main BWA simulants, to implement a spectral signature database and apply the Universal Multi Event Locator (UMEL) statistical method. Our preliminary analysis, conducted in laboratory conditions with a standard UV lamp source, considers the main experimental setups influencing the fluorescence signature of some of the most commonly used BWA simulants. Our work represents a first step towards the implementation of a spectral database and a laser-based biological stand-off detection and identification technique.

Rapid detection of Staphylococcus aureus in dairy and meat foods by combination of capture with silica-coated magnetic nanoparticles and thermophilic helicase-dependent isothermal amplification.

PubMed

Chen, Xingxing; Wu, Xiaoli; Gan, Min; Xu, Feng; He, Lihua; Yang, Dong; Xu, Hengyi; Shah, Nagendra P; Wei, Hua

2015-03-01

Staphylococcus aureus is one of the main pathogens in dairy and meat products; therefore, developing a highly sensitive and rapid method for its detection is necessary. In this study, a quantitative detection method for Staph. aureus was developed using silica-coated magnetic nanoparticles and thermophilic helicase-dependent isothermal amplification. First, genomic DNA was extracted from lysed bacteria using silica-coated magnetic nanoparticles and amplified using thermophilic helicase-dependent isothermal amplification. After adding the nucleic-acid dye SYBR Green I to the amplicons, the fluorescence intensity was observed using a UV lamp or recorded using a fluorescence spectrophotometer. This detection system had a detection limit of 5×10(0) cfu/mL in pure culture and milk-powder samples and 5×10(1) cfu/mL in pork samples using a UV light in less than 2h. In addition, a good linear relationship was obtained between fluorescence intensity and bacterial concentrations ranging from 10(2) to 10(4) cfu/mL under optimal conditions. Furthermore, the results from contaminated milk powder and pork samples suggested that the detection system could be used for the quantitative analysis of Staph. aureus and applied potentially to the food industry for the detection of this pathogen. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

A new technology for automatic identification and sorting of plastics for recycling.

PubMed

Ahmad, S R

2004-10-01

A new technology for automatic sorting of plastics, based upon optical identification of fluorescence signatures of dyes, incorporated in such materials in trace concentrations prior to product manufacturing, is described. Three commercial tracers were selected primarily on the basis of their good absorbency in the 310-370 nm spectral band and their identifiable narrow-band fluorescence signatures in the visible band of the spectrum when present in binary combinations. This absorption band was selected because of the availability of strong emission lines in this band from a commercial Hg-arc lamp and high fluorescence quantum yields of the tracers at this excitation wavelength band. The plastics chosen for tracing and identification are HDPE, LDPE, PP, EVA, PVC and PET and the tracers were compatible and chemically non-reactive with the host matrices and did not affect the transparency of the plastics. The design of a monochromatic and collimated excitation source, the sensor system are described and their performances in identifying and sorting plastics doped with tracers at a few parts per million concentration levels are evaluated. In an industrial sorting system, the sensor was able to sort 300 mm long plastic bottles at a conveyor belt speed of 3.5 m.sec(-1) with a sorting purity of -95%. The limitation was imposed due to mechanical singulation irregularities at high speed and the limited processing speed of the computer used.

10 CFR Appendix V to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Ceiling Fan Light Kits

Code of Federal Regulations, 2010 CFR

2010-01-01

... with ceiling fan light kits that have medium screw base sockets shall conform to the requirements... testing pin-based fluorescent lamps packaged with ceiling fan light kits that have pin-based sockets shall... base sockets, measure the efficacy, expressed in lumens per watt, in accordance with the test...

76 FR 25211 - Energy Conservation Program: Test Procedures for Fluorescent Lamp Ballasts

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-04

... 44 U.S.C. 1510. #0; #0;The Code of Federal Regulations is sold by the Superintendent of Documents. #0... the Regulatory Flexibility Act C. Review Under the Paperwork Reduction Act of 1995 D. Review Under the... Conservation Act (42 U.S.C. 6291, et seq.; ``EPCA'' or, ``the Act'') sets forth a variety of provisions...

75 FR 71570 - Energy Conservation Program: Test Procedures for Fluorescent Lamp Ballasts

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-24

... improve energy efficiency. Part A of Title III (42 U.S.C. 6291-6309) establishes the ``Energy Conservation... ballasts (ballasts). (42 U.S.C. 6291(1), (2) and 6292(a)(13)) Under EPCA, the overall program consists... the public an opportunity to present oral and written comments on them. (42 U.S.C. 6293(b)(2)) Finally...

10 CFR Appendix V to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Ceiling Fan Light Kits

Code of Federal Regulations, 2013 CFR

2013-01-01

... for Testing” of DOE's “ENERGY STAR Program Requirements for [Compact Fluorescent Lamps] CFLs,” Version... Specifications for Qualifying Products” of the EPA's “ENERGY STAR Program Requirements for Residential Light... requirements specified in section 4, “CFL Requirements for Testing,” of the “ENERGY STAR Program Requirements...

10 CFR Appendix V to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Ceiling Fan Light Kits

Code of Federal Regulations, 2014 CFR

2014-01-01

... for Testing” of DOE's “ENERGY STAR Program Requirements for [Compact Fluorescent Lamps] CFLs,” Version... Specifications for Qualifying Products” of the EPA's “ENERGY STAR Program Requirements for Residential Light... requirements specified in section 4, “CFL Requirements for Testing,” of the “ENERGY STAR Program Requirements...

10 CFR Appendix V to Subpart B of... - Uniform Test Method for Measuring the Energy Consumption of Ceiling Fan Light Kits

Code of Federal Regulations, 2012 CFR

2012-01-01

... for Testing” of DOE's “ENERGY STAR Program Requirements for [Compact Fluorescent Lamps] CFLs,” Version... Specifications for Qualifying Products” of the EPA's “ENERGY STAR Program Requirements for Residential Light... requirements specified in section 4, “CFL Requirements for Testing,” of the “ENERGY STAR Program Requirements...

Growth of hybrid poplars, white spruce, and jack pine under various artificial lights.

Treesearch

Pamela S. Roberts; J. Zavitkovski

1981-01-01

Describes the energy consumption and biological effects of fluorescent, incandescent, and high pressure sodium lighting on the growth of poplars, white spruce, and jack pine in a greenhouse. At similar light levels the biological effects of all three light sources were similar. The incandescent lamps consumed several times more energy than the other two light...

Electrodeless discharge lamp is easily started, has high stability

NASA Technical Reports Server (NTRS)

Bell, W. E.; Bloom, A. L.

1966-01-01

Electrodeless discharge borosilicate glass lamp is used in various high-resolution optical systems. It is partially charged with krypton, contains small amounts of rubidium, and is enclosed in a hermetically sealed envelope that maintains the lamp at an optimum temperature during discharge. The lamp is quickly started by its excitation coil.

Mist and water condensation inside incubators reduce the efficacy of phototherapy.

PubMed

de Carvalho, Manoel; Torrao, Carolina Turano; Moreira, Maria Elisabeth Lopes

2011-03-01

To measure the irradiance in humidified incubator under three different overhead phototherapy devices. The effective irradiance of three phototherapy devices was assessed by taking a series of irradiance measurements in the illuminated field. Measurements were made with a fixed bandwidth broadband radiometer (380-530 nm). The distance between the light source and the radiometer was 35 cm for the daylight fluorescent lamp, 40 cm for the light emitting diode (LED) and 50 cm for the halogen phototherapy. A double-wall incubator was kept at 36°C and set at three different levels of humidity (60-70%, 80% and equal or above 90%). The irradiance under the overhead daylight fluorescent lamp phototherapy did not change with the increasing humidity. However, above 90% humidity, when water vapour inside the incubator was so saturated to the point of totally condensing in the incubator walls, the measured irradiance decreased 15% of the initial values with the blue LED phototherapy and 45% with the halogen spotlight phototherapy. Highly humidified incubators are frequently used to treat very low birthweight infants. Health professionals should be aware that mist and water condensation inside an incubator may significantly reduce the efficacy of treatment.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gordon, Kelly L.; Foster, Rebecca; McGowan, Terry

This article for a building trade magazine describes a national design competition for energy efficient lighting sponsored by the U.S. Department of Energy, the American Lighting Association, and the Consortium for Energy Efficiency, with winners announced at ALA's Annual Conference May 14, 2004, in Tucson. The Lighting for Tomorrow competition was the first national lighting fixture design competition focusing on energy-efficient residential lighting. The competition invited fixture manufacturers and designers to come up with beautiful, functional lighting fixtures that also happen to be energy efficient. Fixtures were required to use a ''dedicated'' energy-efficient light source, such as a pin-based fluorescentmore » lamp that cannot be replaced with a screw-in incandescent bulb. Fixtures also had to meet a minimum energy efficiency level that eliminated use of incandescent and halogen lamps, leaving the door open only to fluorescent sources and LEDs. More than 150 paper designs were submitted in the first phase of the competition, in 2003. Of those, 24 finalists were invited to submit working prototypes in 2004, and the winners were announced in May. The Grand Prize of $10,000 went to American Fluorescent of Waukegan, Illinois, for its ''Salem'' chandelier. Some winning fixtures are already available through Lowe's Home Improvement Centers.« less

Electronic Dimmable Ballasts for High-Intensity Discharge Sodium Vapor and Metal Halide Lamps

NASA Technical Reports Server (NTRS)

Boulanger, Richard

2002-01-01

Two types of high-intensity discharge lamps were tested using dimmable ballasts. The main purpose for evaluating this lighting system was to determine its efficacy for saving power. Whereas previous variable level lighting systems for HID lamps in Advanced Life Support applications were adjustable in two or three steps using capacitive switching, this system allows for continuously adjustable lamp output. This type of lighting system when used as part of an Advanced Life Support biomass production system would provide only the amount of light energy a crop needed at any particular point in its growth cycle. Since most of the equivalent system mass in an ALS system is from the light energy required to grow the crops, controlling that light energy dynamically over a continuous range of operation would dramatically reduce the power consumption and reduce system mass.

The modification of spectral characteristics of cytostatics by optical beams

NASA Astrophysics Data System (ADS)

Pascu, Mihail Lucian; Brezeanu, Mihail; Carstocea, Benone D.; Voicu, Letitia; Gazdaru, Doina M.; Smarandache, Adriana A.

2004-10-01

Besides the biochemical action of methotrexate (MTX) and 5-fluorouracil (FU) their effect in destroying cancer tumours could be enhanced by exposure to light at different doses. Absorption, excitation and emission spectra of 10-4M - 10-5M MTX solutions in natural saline and sodium hydroxide at pH = 8.4 were measured, while their exposure to coherent and uncoherent light in the visible and near ultraviolet (UV) spectral ranges was made (Hg lamps and Nitrogen pulsed laser radiation were used). Absorption spectra exhibit spectral bands in the range 200 nm - 450 nm. The 200 - 450 nm excitation spectra were measured with emission centered on 470 nm; MTX fluorescence excitation was measured at 390 nm and the emission was detected between 400 nm and 600 nm showing a maximum at 470 nm. Spectra modifications, nonlinearly depending on exposure time (varying from 1 min to 20 min), evidenced MTX photo-dissociation to the fluorescent compound 2,4 diamino-formylpteridine. In the 5-FU case the absorption spectra exhibit bands between 200 nm and 450 nm. The emission fluorescence spectra were measured between 400 nm and 600 nm, with λex = 350 nm for UV Hg lamp and with λex = 360 nm for laser irradiated samples; at irradiation with N2 laser emitted radiation the excitation spectra were measured in the range of 200 nm - 400 nm, with λem = 440 nm. New vascularity rapid destruction was observed for conjunctive impregnated with 5-FU solution whilst exposed to incoherent UV and visible light.

Self-priming compartmentalization digital LAMP for point-of-care.

PubMed

Zhu, Qiangyuan; Gao, Yibo; Yu, Bingwen; Ren, Hao; Qiu, Lin; Han, Sihai; Jin, Wei; Jin, Qinhan; Mu, Ying

2012-11-21

Digital nucleic acid amplification provides unprecedented opportunities for absolute nucleic acid quantification by counting of single molecules. This technique is useful for molecular genetic analysis in cancer, stem cell, bacterial, non-invasive prenatal diagnosis in which many biologists are interested. This paper describes a self-priming compartmentalization (SPC) microfluidic chip platform for performing digital loop-mediated amplification (LAMP). The energy for the pumping is pre-stored in the degassed bulk PDMS by exploiting the high gas solubility of PDMS; therefore, no additional structures other than channels and reservoirs are required. The sample and oil are sequentially sucked into the channels, and the pressure difference of gas dissolved in PDMS allows sample self-compartmentalization without the need for further chip manipulation such as with pneumatic microvalves and control systems, and so on. The SPC digital LAMP chip can be used like a 384-well plate, so, the world-to-chip fluidic interconnections are avoided. The microfluidic chip contains 4 separate panels, each panel contains 1200 independent 6 nL chambers and can be used to detect 4 samples simultaneously. Digital LAMP on the microfluidic chip was tested quantitatively by using β-actin DNA from humans. The self-priming compartmentalization behavior is roughly predictable using a two-dimensional model. The uniformity of compartmentalization was analyzed by fluorescent intensity and fraction of volume. The results showed that the feasibility and flexibility of the microfluidic chip platform for amplifying single nucleic acid molecules in different chambers made by diluting and distributing sample solutions. The SPC chip has the potential to meet the requirements of a general laboratory: power-free, valve-free, operating at isothermal temperature, inexpensive, sensitive, economizing labour time and reagents. The disposable analytical devices with appropriate air-tight packaging should be useful for point-of-care, and enabling it to become one of the common tools for biology research, especially, in point-of-care testing.

46 CFR 167.45-1 - Steam, carbon dioxide, and halon fire extinguishing systems.

Code of Federal Regulations, 2010 CFR

2010-10-01

... accepted in lieu of the inert gas system for the protection of cargo holds, paint lockers, and similar... to each cargo-oil deep tank, lamp locker, oil room, and like compartments, which lamp locker, oil... lamp lockers, oil rooms, and like compartments may be taken from the nearest steam supply line...

46 CFR 167.45-1 - Steam, carbon dioxide, and halon fire extinguishing systems.

Code of Federal Regulations, 2011 CFR

2011-10-01

... accepted in lieu of the inert gas system for the protection of cargo holds, paint lockers, and similar... to each cargo-oil deep tank, lamp locker, oil room, and like compartments, which lamp locker, oil... lamp lockers, oil rooms, and like compartments may be taken from the nearest steam supply line...

Real-time and quantitative fluorescent live-cell imaging with quadruplex-specific red-edge probe (G4-REP).

PubMed

Yang, Sunny Y; Amor, Souheila; Laguerre, Aurélien; Wong, Judy M Y; Monchaud, David

2017-05-01

The development of quadruplex-directed molecular diagnostic and therapy rely on mechanistic insights gained at both cellular and tissue levels by fluorescence imaging. This technique is based on fluorescent reporters that label cellular DNA and RNA quadruplexes to spatiotemporally address their complex cell biology. The photophysical characteristics of quadruplex probes usually dictate the modality of cell imaging by governing the selection of the light source (lamp, LED, laser), the optical light filters and the detection modality. Here, we report the characterizations of prototype from a new generation of quadruplex dye termed G4-REP (for quadruplex-specific red-edge probe) that provides fluorescence responses regardless of the excitation wavelength and modality (owing to the versatility gained through the red-edge effect), thus allowing for diverse applications and most imaging facilities. This is demonstrated by cell images (and associated quantifications) collected through confocal and multiphoton microscopy as well as through real-time live-cell imaging system over extended period, monitoring both non-cancerous and cancerous human cell lines. Our results promote a new way of designing versatile, efficient and convenient quadruplex-reporting dyes for tracking these higher-order nucleic acid structures in living human cells. This article is part of a Special Issue entitled "G-quadruplex" Guest Editor: Dr. Concetta Giancola and Dr. Daniela Montesarchio. Copyright © 2016 Elsevier B.V. All rights reserved.

Quenching of unincorporated amplification signal reporters in reverse-transcription loop-mediated isothermal amplification enabling bright, single-step, closed-tube, and multiplexed detection of RNA viruses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ball, Cameron S.; Light, Yooli K.; Koh, Chung -Yan

Reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) has frequently been proposed as an enabling technology for simplified diagnostic tests for RNA viruses. However, common detection techniques used for LAMP and RT-LAMP have drawbacks, including poor discrimination capability, inability to multiplex targets, high rates of false positives, and (in some cases) the requirement of opening reaction tubes postamplification. Here, we present a simple technique that allows closed-tube, target-specific detection, based on inclusion of a dye-labeled primer that is incorporated into a target-specific amplicon if the target is present. A short, complementary quencher hybridizes to unincorporated primer upon cooling down at the end of themore » reaction, thereby quenching fluorescence of any unincorporated primer. Our technique, which we term QUASR (for quenching of unincorporated amplification signal reporters, read “quasar”), does not significantly reduce the amplification efficiency or sensitivity of RT-LAMP. Equipped with a simple LED excitation source and a colored plastic gel filter, the naked eye or a camera can easily discriminate between positive and negative QUASR reactions, which produce a difference in signal of approximately 10:1 without background subtraction. We demonstrate that QUASR detection is compatible with complex sample matrices such as human blood, using a novel LAMP primer set for bacteriophage MS2 (a model RNA virus particle). As a result, we demonstrate single-tube duplex detection of West Nile virus (WNV) and chikungunya virus (CHIKV) RNA.« less

Quenching of unincorporated amplification signal reporters in reverse-transcription loop-mediated isothermal amplification enabling bright, single-step, closed-tube, and multiplexed detection of RNA viruses

DOE PAGES

Ball, Cameron S.; Light, Yooli K.; Koh, Chung -Yan; ...

2016-03-16

Reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) has frequently been proposed as an enabling technology for simplified diagnostic tests for RNA viruses. However, common detection techniques used for LAMP and RT-LAMP have drawbacks, including poor discrimination capability, inability to multiplex targets, high rates of false positives, and (in some cases) the requirement of opening reaction tubes postamplification. Here, we present a simple technique that allows closed-tube, target-specific detection, based on inclusion of a dye-labeled primer that is incorporated into a target-specific amplicon if the target is present. A short, complementary quencher hybridizes to unincorporated primer upon cooling down at the end of themore » reaction, thereby quenching fluorescence of any unincorporated primer. Our technique, which we term QUASR (for quenching of unincorporated amplification signal reporters, read “quasar”), does not significantly reduce the amplification efficiency or sensitivity of RT-LAMP. Equipped with a simple LED excitation source and a colored plastic gel filter, the naked eye or a camera can easily discriminate between positive and negative QUASR reactions, which produce a difference in signal of approximately 10:1 without background subtraction. We demonstrate that QUASR detection is compatible with complex sample matrices such as human blood, using a novel LAMP primer set for bacteriophage MS2 (a model RNA virus particle). As a result, we demonstrate single-tube duplex detection of West Nile virus (WNV) and chikungunya virus (CHIKV) RNA.« less

The Chemistry and Perception of Fluorescent White Textile Materials

NASA Astrophysics Data System (ADS)

Xu, Changhai

Cationic bleach activators (CBA) are precursors to perhydroxyl compounds that, when activated, have higher oxidation potential and potentially improved bleaching performance compared to common oxidizing agents such as hydrogen peroxide. CBAs were first reported in 1997 by Proctor & Gamble Co., and have been further developed at North Carolina State University. To date, an effective low temperature bleaching system has not been developed that offers sufficient economic improvement over existing bleaching systems. The primary purpose of this research was to develop new methods and understand key variables required for achieving enhanced whiteness of textile materials using bleach activators with or without the presence of fluorescence. A new optimized low temperature bleaching system using novel lactam-based bleach activators was developed and the effect of UV content of light sources on the whiteness of fluorescent white textile materials was evaluated. A novel class of bleach activators was synthesized by introducing benzoylcaprolactam group into a stilbene system shown as follows:* While solubility, purification and hydrolytic stability of the compounds were problematic, a new approach to low pH (pH 7-9) and low temperature (50-70°C) bleaching was found using a butyrolactam-based cationic bleach activator, N-[4-(triethylammonio methyl)benzoyl]-butyrolactam chloride (TBBC), using a central composite design (CCD) of experiment. The CCD bleaching experiments showed that cationic bleach activators are less effective with high concentrations of hydrogen peroxide in high alkaline solutions. Also a 2FI model predicted the optimized bleaching performance on 100% cotton at near neutral pH and temperatures around 50°C, in which the dosage of TBBC is the most important factor affecting the bleaching performance. This prediction was validated experimentally during bleaching of bamboo and cotton fibers. In addition, this study confirmed the hypothesis that cationic bleach activators have inherent substantivity to cellulosic fibers and that the substantivity enhances bleach effectiveness. This cationic activated bleach system was effective for cotton bleaching and the bleaching performance is superior to that of conventional peroxide bleaching. TBBC was also applied to bamboo cellulosic fibers, which exhibit excessive strength loss during conventional hot bleaching. Under optimized conditions of 5 g/L TBBC, 50°C, 30 min and pH 7.0, TBBC-based bleaching of bamboo fibers produced CIE Whiteness Index (CIE WI) values of 58.20 compared to untreated bamboo which had CIE WI values of 10.77. Hence, the TBBC bleach activator method is effective at bleaching bamboo fibers. Besides chemical bleaching, the fluorescent whitening was another approach to increase the whiteness of materials. Since the whiteness of fluorescent white materials is produced by absorbing UV light and emitting visible blue light, the UV content of light sources has a significant effect on the perception of whiteness. This research addresses the common light sources used in color matching booths. The pilot data is collected by measuring spectral radiance and spectral irradiance, which is used for analysis of the UV effect on the whiteness of fluorescent white materials. The whitening performance of a fluorescent brightening agent (FBA) is dependent on the energy and intensity of the incident UV light. No data have been reported in the open literature that shows the UV emission of standard lamps used in viewing booths. Indeed, standards pertaining to lighting do not require or recommend the standardization of the UV content in any lamps. Hence, the spectroradiometric quantification of UV emission of a series of standard viewing booths is a requirement for establishment of a methodology to determine the effect of radiometric variability in standard sources on visual perception of fluorescent white materials. The radiance measurement data collected from measuring the radiance of light sources (including daylight simulation, incandescent, horizon daylight, cool white fluorescence, and Ultralume 30) in a SpectraLight III color viewing booth and the irradiance of these light sources over a PTFE diffuse reflectance standard, AATCC textile UV calibration standard and some fluorescent whitened fabric samples showed the inadequacy of UV content of these light sources in the SpectraLight III. *Please refer to dissertation for diagram.

LRO-LAMP observations of the LCROSS impact plume.

PubMed

Gladstone, G Randall; Hurley, Dana M; Retherford, Kurt D; Feldman, Paul D; Pryor, Wayne R; Chaufray, Jean-Yves; Versteeg, Maarten; Greathouse, Thomas K; Steffl, Andrew J; Throop, Henry; Parker, Joel Wm; Kaufmann, David E; Egan, Anthony F; Davis, Michael W; Slater, David C; Mukherjee, Joey; Miles, Paul F; Hendrix, Amanda R; Colaprete, Anthony; Stern, S Alan

2010-10-22

On 9 October 2009, the Lunar Crater Observation and Sensing Satellite (LCROSS) sent a kinetic impactor to strike Cabeus crater, on a mission to search for water ice and other volatiles expected to be trapped in lunar polar soils. The Lyman Alpha Mapping Project (LAMP) ultraviolet spectrograph onboard the Lunar Reconnaissance Orbiter (LRO) observed the plume generated by the LCROSS impact as far-ultraviolet emissions from the fluorescence of sunlight by molecular hydrogen and carbon monoxide, plus resonantly scattered sunlight from atomic mercury, with contributions from calcium and magnesium. The observed light curve is well simulated by the expansion of a vapor cloud at a temperature of ~1000 kelvin, containing ~570 kilograms (kg) of carbon monoxide, ~140 kg of molecular hydrogen, ~160 kg of calcium, ~120 kg of mercury, and ~40 kg of magnesium.

Emergency Lighting System

NASA Technical Reports Server (NTRS)

1994-01-01

When power outages occurred at Landmark Plastic Corporation, it took seven to twelve minutes for the primary mercury lamps to cool down enough to relight and two to seven minutes for the ELS incandescent lamps to relight. Production could not resume for as much as seven minutes. An article in NASA Tech Briefs describing the capabilities of photosensing devices led Landmark employee, Steve Keller to design a system now activated by any voltage loss in the main lamp circuit and coupled with photosensing devices used to keep them on until the primary mercury lamps reach full brightness.

Development of multiwavelength excitation light source for autofluorescence and photodynamic diagnosis systems

NASA Astrophysics Data System (ADS)

Kenar, Necla; Lim, H. S.; Mirzaaghasi, Amin

2014-02-01

New design of the excitation light source that can stably generate light with center wavelengths of 450nm, 530nm, 632.8nm and white light for auto-fluorescence(AF) and photodynamic diagnosis(PDD) of cancer in clinics in a single system is presented in this study. The light source consists of Xenon Lamp (300W), light guide module including motorize filter wheel equipped with optical filters with corresponding to wavelength bands, servo motor, motorize iris, a cooling system, power supply and optical transmission part for the output light. The transmission part of the light source was developed to collimate the light with desired wavelength into input of fiber optic. Output powers are obtained average 99.91 mW for 450+/-40 nm, 111.01 mW for 530+/-10nm, and 78.50 mW for 632.8+/-10nm.

Hantavirus Gc induces long-term immune protection via LAMP-targeting DNA vaccine strategy.

PubMed

Jiang, Dong-Bo; Zhang, Jin-Peng; Cheng, Lin-Feng; Zhang, Guan-Wen; Li, Yun; Li, Zi-Chao; Lu, Zhen-Hua; Zhang, Zi-Xin; Lu, Yu-Chen; Zheng, Lian-He; Zhang, Fang-Lin; Yang, Kun

2018-02-01

Hemorrhagic fever with renal syndrome (HFRS) occurs widely throughout Eurasia. Unfortunately, there is no effective treatment, and prophylaxis remains the best option against the major pathogenic agent, hantaan virus (HTNV), which is an Old World hantavirus. However, the absence of cellular immune responses and immunological memory hampers acceptance of the current inactivated HFRS vaccine. Previous studies revealed that a lysosome-associated membrane protein 1 (LAMP1)-targeting strategy involving a DNA vaccine based on the HTNV glycoprotein Gn successfully conferred long-term immunity, and indicated that further research on Gc, another HTNV antigen, was warranted. Plasmids encoding Gc and lysosome-targeted Gc, designated pVAX-Gc and pVAX-LAMP/Gc, respectively, were constructed. Proteins of interest were identified by fluorescence microscopy following cell line transfection. Five groups of 20 female BALB/c mice were subjected to the following inoculations: inactivated HTNV vaccine, pVAX-LAMP/Gc, pVAX-Gc, and, as the negative controls, pVAX-LAMP or the blank vector pVAX1. Humoral and cellular immunity were assessed by enzyme-linked immunosorbent assays (ELISAs) and 15-mer peptide enzyme-linked immunospot (ELISpot) epitope mapping assays. Repeated immunization with pVAX-LAMP/Gc enhanced adaptive immune responses, as demonstrated by the specific and neutralizing antibody titers and increased IFN-γ production. The inactivated vaccine induced a comparable humoral reaction, but the negative controls only elicited insignificant responses. Using a mouse model of HTNV challenge, the in vivo protection conferred by the inactivated vaccine and Gc-based constructs (with/without LAMP recombination) was confirmed. Evidence of pan-epitope reactions highlighted the long-term cellular response to the LAMP-targeting strategy, and histological observations indicated the safety of the LAMP-targeting vaccines. The long-term protective immune responses induced by pVAX-LAMP/Gc may be due to the advantage afforded by lysosomal targeting after exogenous antigen processing initiation and major histocompatibility complex (MHC) class II antigen presentation trafficking. MHC II-restricted antigen recognition effectively primes HTNV-specific CD4 + T-cells, leading to the promotion of significant immune responses and immunological memory. An epitope-spreading phenomenon was observed, which mirrors the previous result from the Gn study, in which the dominant IFN-γ-responsive hot-spot epitopes were shared between HLA-II and H2 d . Importantly, the pan-epitope reaction to Gc indicated that Gc should be with potential for use in further hantavirus DNA vaccine investigations. Copyright © 2017 Elsevier B.V. All rights reserved.

Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malaria.

PubMed

Lucchi, Naomi W; Demas, Allison; Narayanan, Jothikumar; Sumari, Deborah; Kabanywanyi, Abdunoor; Kachur, S Patrick; Barnwell, John W; Udhayakumar, Venkatachalam

2010-10-29

Molecular diagnostic methods can complement existing tools to improve the diagnosis of malaria. However, they require good laboratory infrastructure thereby restricting their use to reference laboratories and research studies. Therefore, adopting molecular tools for routine use in malaria endemic countries will require simpler molecular platforms. The recently developed loop-mediated isothermal amplification (LAMP) method is relatively simple and can be improved for better use in endemic countries. In this study, we attempted to improve this method for malaria diagnosis by using a simple and portable device capable of performing both the amplification and detection (by fluorescence) of LAMP in one platform. We refer to this as the RealAmp method. Published genus-specific primers were used to test the utility of this method. DNA derived from different species of malaria parasites was used for the initial characterization. Clinical samples of P. falciparum were used to determine the sensitivity and specificity of this system compared to microscopy and a nested PCR method. Additionally, directly boiled parasite preparations were compared with a conventional DNA isolation method. The RealAmp method was found to be simple and allowed real-time detection of DNA amplification. The time to amplification varied but was generally less than 60 minutes. All human-infecting Plasmodium species were detected. The sensitivity and specificity of RealAmp in detecting P. falciparum was 96.7% and 91.7% respectively, compared to microscopy and 98.9% and 100% respectively, compared to a standard nested PCR method. In addition, this method consistently detected P. falciparum from directly boiled blood samples. This RealAmp method has great potential as a field usable molecular tool for diagnosis of malaria. This tool can provide an alternative to conventional PCR based diagnostic methods for field use in clinical and operational programs.

Interpretation of digital chest radiographs: comparison of light emitting diode versus cold cathode fluorescent lamp backlit monitors.

PubMed

Lim, Hyun-ju; Chung, Myung Jin; Lee, Geewon; Yie, Miyeon; Shin, Kyung Eun; Moon, Jung Won; Lee, Kyung Soo

2013-01-01

To compare the diagnostic performance of light emitting diode (LED) backlight monitors and cold cathode fluorescent lamp (CCFL) monitors for the interpretation of digital chest radiographs. We selected 130 chest radiographs from health screening patients. The soft copy image data were randomly sorted and displayed on a 3.5 M LED (2560 × 1440 pixels) monitor and a 3 M CCFL (2048 × 1536 pixels) monitor. Eight radiologists rated their confidence in detecting nodules and abnormal interstitial lung markings (ILD). Low dose chest CT images were used as a reference standard. The performance of the monitor systems was assessed by analyzing 2080 observations and comparing them by multi-reader, multi-case receiver operating characteristic analysis. The observers reported visual fatigue and a sense of heat. Radiant heat and brightness of the monitors were measured. Measured brightness was 291 cd/m(2) for the LED and 354 cd/m(2) for the CCFL monitor. Area under curves for nodule detection were 0.721 ± 0.072 and 0.764 ± 0.098 for LED and CCFL (p = 0.173), whereas those for ILD were 0.871 ± 0.073 and 0.844 ± 0.068 (p = 0.145), respectively. There were no significant differences in interpretation time (p = 0.446) or fatigue score (p = 0.102) between the two monitors. Sense of heat was lower for the LED monitor (p = 0.024). The temperature elevation was 6.7℃ for LED and 12.4℃ for the CCFL monitor. Although the LED monitor had lower maximum brightness compared with the CCFL monitor, soft copy reading of the digital chest radiographs on LED and CCFL showed no difference in terms of diagnostic performance. In addition, LED emitted less heat.

Feasibility of light-emitting diode uses for annular reactor inner-coated with TiO2 or nitrogen-doped TiO2 for control of dimethyl sulfide.

PubMed

Jo, Wan-Kuen; Eun, Sung-Soo; Shin, Seung-Ho

2011-01-01

Limited environmental pollutants have only been investigated for the feasibility of light-emitting diodes (LED) uses in photocatalytic decomposition (PD). The present study investigated the applicability of LEDs for annular photocatalytic reactors by comparing PD efficiencies of dimethyl sulfide (DMS), which has not been investigated with any LED-PD system, between photocatalytic systems utilizing conventional and various LED lamps with different wavelengths. A conventional 8 W UV/TiO(2) system exhibited a higher DMS PD efficiency as compared with UV-LED/TiO(2) system. Similarly, a conventional 8 W visible-lamp/N-enhanced TiO(2) (NET) system exhibited a higher PD efficiency as compared with six visible-LED/NET systems. However, the ratios of PD efficiency to the electric power consumption were rather high for the photocatalytic systems using UV- or visible-LED lamps, except for two LED lamps (yellow- and red-LED lamps), compared to the photocatalytic systems using conventional lamps. For the photocatalytic systems using LEDs, lower flow rates and input concentrations and shorter hydraulic diameters exhibited higher DMS PD efficiencies. An Fourier-transformation infrared analysis suggested no significant absorption of byproducts on the catalyst surface. Consequently, it was suggested that LEDs can still be energy-efficiently utilized as alternative light sources for the PD of DMS, under the operational conditions used in this study. © 2011 The Authors. Photochemistry and Photobiology © 2011 The American Society of Photobiology.

Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA

DOE PAGES

Meagher, Robert J.; Priye, Aashish; Light, Yooli K.; ...

2018-03-27

Loop-mediated isothermal amplification (LAMP), coupled with reverse transcription (RT), has become a popular technique for detection of viral RNA due to several desirable characteristics for use in point-of-care or low-resource settings. The large number of primers in LAMP (six per target) leads to an increased likelihood of primer-dimer interactions, and the inner primers in particular are prone to formation of stable hairpin structures due to their length (typically 40-45 bases). Although primer-dimers and hairpin structures are known features to avoid in nucleic acid amplification techniques, there is little quantitative information in literature regarding the impact of these structures on LAMPmore » or RT-LAMP assays. In this study, we examine the impact of primer-dimers and hairpins on previously-published primer sets for dengue virus and yellow fever virus. We demonstrate that minor changes to the primers to eliminate amplifiable primer dimers and hairpins improves the performance of the assays when monitored in real time with intercalating dyes, and when monitoring a fluorescent endpoint using the QUASR technique. We also discuss the thermodynamic implications of these minor changes on the overall stability of amplifiable secondary structures, and we present a single thermodynamic parameter to predict the probability of non-specific amplification associated with LAMP primers.« less

Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meagher, Robert J.; Priye, Aashish; Light, Yooli K.

Loop-mediated isothermal amplification (LAMP), coupled with reverse transcription (RT), has become a popular technique for detection of viral RNA due to several desirable characteristics for use in point-of-care or low-resource settings. The large number of primers in LAMP (six per target) leads to an increased likelihood of primer-dimer interactions, and the inner primers in particular are prone to formation of stable hairpin structures due to their length (typically 40-45 bases). Although primer-dimers and hairpin structures are known features to avoid in nucleic acid amplification techniques, there is little quantitative information in literature regarding the impact of these structures on LAMPmore » or RT-LAMP assays. In this study, we examine the impact of primer-dimers and hairpins on previously-published primer sets for dengue virus and yellow fever virus. We demonstrate that minor changes to the primers to eliminate amplifiable primer dimers and hairpins improves the performance of the assays when monitored in real time with intercalating dyes, and when monitoring a fluorescent endpoint using the QUASR technique. We also discuss the thermodynamic implications of these minor changes on the overall stability of amplifiable secondary structures, and we present a single thermodynamic parameter to predict the probability of non-specific amplification associated with LAMP primers.« less

Development and clinical performance of high throughput loop-mediated isothermal amplification for detection of malaria

PubMed Central

Perera, Rushini S.; Ding, Xavier C.; Tully, Frank; Oliver, James; Bright, Nigel; Bell, David; Chiodini, Peter L.; Gonzalez, Iveth J.; Polley, Spencer D.

2017-01-01

Background Accurate and efficient detection of sub-microscopic malaria infections is crucial for enabling rapid treatment and interruption of transmission. Commercially available malaria LAMP kits have excellent diagnostic performance, though throughput is limited by the need to prepare samples individually. Here, we evaluate the clinical performance of a newly developed high throughput (HTP) sample processing system for use in conjunction with the Eiken malaria LAMP kit. Methods The HTP system utilised dried blood spots (DBS) and liquid whole blood (WB), with parallel sample processing of 94 samples per run. The system was evaluated using 699 samples of known infection status pre-determined by gold standard nested PCR. Results The sensitivity and specificity of WB-HTP-LAMP was 98.6% (95% CI, 95.7–100), and 99.7% (95% CI, 99.2–100); sensitivity of DBS-HTP-LAMP was 97.1% (95% CI, 93.1–100), and specificity 100% against PCR. At parasite densities greater or equal to 2 parasites/μL, WB and DBS HTP-LAMP showed 100% sensitivity and specificity against PCR. At densities less than 2 p/μL, WB-HTP-LAMP sensitivity was 88.9% (95% CI, 77.1–100) and specificity was 99.7% (95% CI, 99.2–100); sensitivity and specificity of DBS-HTP-LAMP was 77.8% (95% CI, 54.3–99.5) and 100% respectively. Conclusions The HTP-LAMP system is a highly sensitive diagnostic test, with the potential to allow large scale population screening in malaria elimination campaigns. PMID:28166235

78 FR 16443 - Energy Efficiency Program for Consumer Products: Energy Conservation Standards for Ceiling Fans...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-15

... with medium screw base sockets, as well as pin-based sockets. (42 U.S.C. 6295(ff)(2)-(3)) The statute... (including candelabra screw base sockets) by January 1, 2007, and if DOE failed to issue such standards by... standards for light kits with sockets other than medium-screw base or pin-based fluorescent lamps in the CFR...

Effect of light-emitting diode vs. fluorescent lighting on laying hens in aviary hen houses: Part 1 - Operational characteristics of lights and production traits of hens.

PubMed

Long, H; Zhao, Y; Wang, T; Ning, Z; Xin, H

2016-01-01

Light-emitting diode (LED) lights are becoming more affordable for agricultural applications. Despite many lab-scale studies concerning impact of LED on poultry, little research has been documented under field production conditions, especially for laying hens. This 15-month field study was carried out to evaluate the effects of LED vs. fluorescent (FL) lights on laying hens (Dekalb white breed) using 4 (2 pairs) aviary hen houses each at a nominal capacity of 50,000 hens. The evaluation was done regarding operational characteristics of the lights and hen production traits. The results show that spatial distribution of the LED light was less uniform than that of the FL light. Light intensity of the LED light decreased by 27% after 3,360 h use but remained quite steady from 3,360 to 5,760 h use. Eleven out of 762 (1.44%) LED lamps (new at onset of the study) in the 2 houses failed during the 15-month experiment period. The neck area of the LED lamp was hottest, presumably the primary reason for the lamp failure as cracks were noticed in the neck region of all failed LED lamps. No differences were observed in egg weight, hen-day egg production, feed use, and mortality rate between LED and FL regimens. However, hens under the FL had higher eggs per hen housed and better feed conversion than those under the LED during 20 to 70 wk production (P < 0.05). Hens under the LED tended to have less feather uniformity and insulation than those under the FL (P < 0.05). Moreover, hens under the LED showed a larger median avoidance distance than those under the FL at 36 wk age (P < 0.05), indicating that hens under the LED were more alert; but no difference at 60 wk age. More comparative research to quantify behavioral and production responses of different breeds of hens to LED vs. FL lighting seems warranted. © 2015 Poultry Science Association Inc.

Infrared fluorescence microscopy of stained tissues: principles and technic.

PubMed

Puchtler, H; Meloan, S N; Paschal, L D

1980-01-01

Infrared photomicrography was used extensively from 1927 to the 1940's, but received little attention during the last decades. However, studies of infrared fluorescence of stained sections could not be found in the accessible literature. Ramsley (1968) published quantitative data on infrared fluorescence of approximately 250 dyes bound to textile fibers. The intensity of infrared fluorescence of many dyes varied widely with the substrate. It was therefore deemed of interest to determine whether or not similar differences in infrared fluorescence may occur when dyes are bound to histochemically distinct tissue structures. Myofibrils and collagens stained with triarylmethane dyes were chosen as test objects. Kodak infrared cut-off filter No. 301 and Wratten filter #16 were used as exciter filters to remove infrared and UV-blue and the light of a xenon lamp. Wratten filter #70 and #89B were employed as barrier filters. Infrared radiation was recorded with Kodak Ektachrome infrared film. To facilitate correlation of infrared fluorescence patterns with visible images, tissues were photographed also with conventional color film. Stained myofibrils, e.g. in myoepithelium, smooth and striated muscle emitted strong infrared fluorescence; collagen showed little or no fluorescence. Barrier filter Wratten #70 permitted simultaneous demonstration of infrared fluorescence and of non-fluorescent structures and thus facilitated histopathological studies. Preliminary findings indicate decrease or loss of infrared fluorescence of stained muscle fibers in various lesions, e.g. myocardial infarction, Duchenne-type muscular dystrophy.

LAMP-2 mediates oxidative stress-dependent cell death in Zn2+-treated lung epithelium cells.

PubMed

Qin, Xia; Zhang, Jun; Wang, Bin; Xu, Ge; Zou, Zhen

2017-06-17

Zinc is an essential element for the biological system. However, excessive exogenous Zn 2+ would disrupt cellular Zn 2+ homeostasis and cause toxicity. In particular, Zinc salts or ZnO nanoparticles exposure could induce respiratory injury. Although previous studies have indicated that organelle damage (including mitochondria or lysosomes) and reactive oxygen species (ROS) production are involved in Zn 2+ -induced toxicity, the interplay between mitochondria/lysosomes damage and ROS production is obscure. Herein, we demonstrated that Zn 2+ could induce deglycosylation of lysosome-associated membrane protein 1 and 2 (LAMP-1 and LAMP-2), which primarily locate in late endosomes/lysosomes, in A549 lung epithelium cells. Intriguingly, LAMP-2 knockdown further aggravated Zn 2+ -mediated ROS production and cell death, indicating LAMP-2 (not LAMP-1) was involved in Zn 2+ -induced toxicity. Our results provide a new insight that LAMP-2 contributes to the ROS clearance and cell death induced by Zn 2+ treatment, which would help us to get a better understanding of Zn 2+ -induced toxicity in respiratory system. Copyright © 2017 Elsevier Inc. All rights reserved.

Interface-Free Area-Scalable Self-Powered Electroluminescent System Driven by Triboelectric Generator

PubMed Central

Yan Wei, Xiao; Kuang, Shuang Yang; Yang Li, Hua; Pan, Caofeng; Zhu, Guang; Wang, Zhong Lin

2015-01-01

Self-powered system that is interface-free is greatly desired for area-scalable application. Here we report a self-powered electroluminescent system that consists of a triboelectric generator (TEG) and a thin-film electroluminescent (TFEL) lamp. The TEG provides high-voltage alternating electric output, which fits in well with the needs of the TFEL lamp. Induced charges pumped onto the lamp by the TEG generate an electric field that is sufficient to excite luminescence without an electrical interface circuit. Through rational serial connection of multiple TFEL lamps, effective and area-scalable luminescence is realized. It is demonstrated that multiple types of TEGs are applicable to the self-powered system, indicating that the system can make use of diverse mechanical sources and thus has potentially broad applications in illumination, display, entertainment, indication, surveillance and many others. PMID:26338365

Lande gJ factors for even-parity electronic levels in the holmium atom

NASA Astrophysics Data System (ADS)

Stefanska, D.; Werbowy, S.; Krzykowski, A.; Furmann, B.

2018-05-01

In this work the hyperfine structure of the Zeeman splitting for 18 even-parity levels in the holmium atom was investigated. The experimental method applied was laser induced fluorescence in a hollow cathode discharge lamp. 20 spectral lines were investigated involving odd-parity levels from the ground multiplet, for which Lande gJ factors are known with high precision, as the lower levels; this greatly facilitated the evaluation of gJ factors for the upper levels. The gJ values for the even-parity levels considered are reported for the first time. They proved to compare fairly well with the values obtained recently in a semi-empirical analysis for the even-parity level system of Ho I.

Optimization of loop-mediated isothermal amplification (LAMP) assays for the detection of Leishmania DNA in human blood samples.

PubMed

Abbasi, Ibrahim; Kirstein, Oscar D; Hailu, Asrat; Warburg, Alon

2016-10-01

Visceral leishmaniasis (VL), one of the most important neglected tropical diseases, is caused by Leishmania donovani eukaryotic protozoan parasite of the genus Leishmania, the disease is prevalent mainly in the Indian sub-continent, East Africa and Brazil. VL can be diagnosed by PCR amplifying ITS1 and/or kDNA genes. The current study involved the optimization of Loop-mediated isothermal amplification (LAMP) for the detection of Leishmania DNA in human blood or tissue samples. Three LAMP systems were developed; in two of those the primers were designed based on shared regions of the ITS1 gene among different Leishmania species, while the primers for the third LAMP system were derived from a newly identified repeated region in the Leishmania genome. The LAMP tests were shown to be sufficiently sensitive to detect 0.1pg of DNA from most Leishmania species. The green nucleic acid stain SYTO16, was used here for the first time to allow real-time monitoring of LAMP amplification. The advantage of real time-LAMP using SYTO 16 over end-point LAMP product detection is discussed. The efficacy of the real time-LAMP tests for detecting Leishmania DNA in dried blood samples from volunteers living in endemic areas, was compared with that of qRT-kDNA PCR. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

Measurement of the Fluorescence Lifetime of Chlorophyll a In Vivo

PubMed Central

Singhal, G. S.; Rabinowitch, E.

1969-01-01

New measurements have been made of fluorescence lifetime (τ) of chlorophyll a in the algae Chlorella pyrenoidosa, Porphyridium cruentum, Anacystis nidulans, and in spinach chloroplast. τ-values of 0.6 and 0.7 nsec were obtained with green plants. Anacystis and Porphyridium gave a τ of 0.5 nsec. The previously described two stage decay of fluorescence in vivo in these organisms could not be confirmed. This observation could have been caused by a second wave of light emission from the exciting hydrogen lamp (not detected in earlier work). The lifetimes found in this study (calculated, as before, by the method of convolution integrals) were close to those found by other observers for “low” excitation intensities; the value first reported from this laboratory (1.0-1.7 nsec) may have corresponded to “high” excitation intensity. PMID:5778187

Stress Testing of the Philips 60W Replacement Lamp L Prize Entry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Poplawski, Michael E.; Ledbetter, Marc R.; Smith, Mark

2012-04-24

The Pacific Northwest National Laboratory, operated by Battelle for the U.S. Department of Energy, worked with Intertek to develop a procedure for stress testing medium screw-base light sources. This procedure, composed of alternating stress cycles and performance evaluation, was used to qualitatively compare and contrast the durability and reliability of the Philips 60W replacement lamp L Prize entry with market-proven compact fluorescent lamps (CFLs) with comparable light output and functionality. The stress cycles applied simultaneous combinations of electrical, thermal, vibration, and humidity stresses of increasing magnitude. Performance evaluations measured relative illuminance, x chromaticity and y chromaticity shifts after each stressmore » cycle. The Philips L Prize entry lamps appear to be appreciably more durable than the incumbent energy-efficient technology, as represented by the evaluated CFLs, and with respect to the applied stresses. Through the course of testing, all 15 CFL samples permanently ceased to function as a result of the applied stresses, while only 1 Philips L Prize entry lamp exhibited a failure, the nature of which was minor, non-destructive, and a consequence of a known (and resolved) subcontractor issue. Given that current CFL technology appears to be moderately mature and no Philips L Prize entry failures could be produced within the stress envelope causing 100 percent failure of the benchmark CFLs, it seems that, in this particular implementation, light-emitting diode (LED) technology would be much more durable in the field than current CFL technology. However, the Philips L Prize entry lamps used for testing were carefully designed and built for the competition, while the benchmark CFLs were mass produced for retail sale—a distinction that should be taken into consideration. Further reliability testing on final production samples would be necessary to judge the extent to which the results of this analysis apply to production versions of the Philips L Prize entry.« less

Studying colours with a smartphone

NASA Astrophysics Data System (ADS)

Rosi, T.; Malgieri, M.; Onorato, P.; De Ambrosis, , A.; Oss, S.

2017-03-01

We show how a low-cost spectrometer, based on the use of inexpensive diffraction transmission gratings coupled with a smartphone photo camera, can be assembled and employed to obtain quantitative measurements of spectra from different sources. The analysis of spectra emitted by different light sources (incandescent bulb, fluorescent lamp, gas lamps, LEDs) helps students understand the different physical mechanisms which govern the production of light. Measurements of emission and transmission spectra allow students to focus on the differences between additive and subtractive models of colour formation. For this purpose the spectra of RGB colours emitted from an LCD screen and the transmission spectra of CMY pigments of a laser printer have been studied, using our low-cost spectroscope. A sequence of experimental activities was designed, and proposed to undergraduate students and secondary school teachers in order to study the feasibility and educational potential.

Phagosome maturation in unicellular eukaryote Paramecium: the presence of RILP, Rab7 and LAMP-2 homologues.

PubMed

Wyroba, E; Surmacz, L; Osinska, M; Wiejak, J

2007-01-01

Phagosome maturation is a complex process enabling degradation of internalised particles. Our data obtained at the gene, protein and cellular level indicate that the set of components involved in this process and known up to now in mammalian cells is functioning in unicellular eukaryote. Rab7-interacting partners: homologues of its effector RILP (Rab-interacting lysosomal protein) and LAMP-2 (lysosomal membrane protein 2) as well as alpha7 subunit of the 26S proteasome were revealed in Paramecium phagolysosomal compartment. We identified the gene/transcript fragments encoding RILP-related proteins (RILP1 and RILP2) in Paramecium by PCR/RT-PCR and sequencing. The deduced amino acid sequences of RILP1 and RILP2 show 60.5% and 58.3% similarity, respectively, to the region involved in regulating of lysosomal morphology and dynein-dynactin recruitment of human RILP. RILP colocalised with Rab7 in Paramecium lysosomes and at phagolysosomal membrane during phagocytosis of both the latex beads and bacteria. In the same compartment LAMP-2 was present and its expression during latex internalisation was 2.5-fold higher than in the control when P2 protein fractions (100,000 x g) of equal load were quantified by immunoblotting. LAMP-2 cross-reacting polypeptide of approximately106 kDa was glycosylated as shown by fluorescent and Western analysis of the same blot preceded by PNGase F treatment. The alpha7 subunit of 26S proteasome was detected close to the phagosomal membrane in the small vesicles, in some of which it colocalised with Rab7. Immunoblotting confirmed presence of RILP-related polypeptide and a7 subunit of 26S proteasome in Paramecium protein fractions. These results suggest that Rab7, RILP and LAMP-2 may be involved in phagosome maturation in Paramecium.

Investigation of the electrical discharge parameters in electrodeless inductive lamps with a re-entrant coupler and magnetic core

NASA Astrophysics Data System (ADS)

Statnic, Eugen; Tanach, Valentin

2006-08-01

The inductively coupled fluorescent lamp with a cored induction coil placed in a re-entrant cavity is in fact a coaxial transformer operated in the radiofrequency range between 100 kHz and a few MHz. The magnetic coupling coefficient k between the primary coil and the plasma ring is relatively low because of the open magnetic circuit. The acting mutual inductance M enables us to quantify the interaction between the magnetic field produced by the primary coil current I1 and the opposing magnetic field produced by the powerful plasma current I2. A contra-electromotive force jωMI2 is induced in the induction coil L1, defining the primary voltage V1 = I1(R1 + jω L1) - jωMI2. The current I1 induces in the secondary conductive plasma the driving electromotive force jωMI1 supplying the secondary load consisting of the average plasma resistance R2 and the secondary inductance L2, according to the equation jωMI2 = I2(R2 + jωL2). It is the aim of this paper to find a model to determine k on the basis of the measured primary parameters V1, I1, P1, L1, R1 and finally all electrical inaccessible parameters, such as M, V2, I2, phiv2, L2, R2, in order to optimize the discharge and lamp efficacy. The complex characteristic of plasma inductance for this type of lamp is analysed and clarified. Some reflexive basic relations verifying the correctness of the inferred plasma parameters are also developed. The described experiments are related to a lamp working at about 2.6 MHz.

Towards a uniform specification of light therapy devices for the treatment of affective disorders and use for non-image forming effects: Radiant flux.

PubMed

Aarts, M P J; Rosemann, A L P

2018-08-01

For treating affective disorders like SAD, light therapy is used although the underlying mechanism explaining this success remains unclear. To accelerate the research on defining the light characteristics responsible for inducing a specific effect a uniform manner for specifying the irradiance at the eye should be defined. This allows a genuine comparison between light-affect studies. An important factor impacting the irradiance at the eye are the radiant characteristics of the used light therapy device. In this study the radiant fluxes of five different light therapy devices were measured. The values were weighted against the spectral sensitivity of the five photopigments present in the human eye. A measurement was taken every five minutes to control for a potential stabilizing effect. The results show that all five devices show large differences in radiant flux. The devices equipped with blue LED lights have a much lower spectral radiant flux than the devices equipped with a fluorescent light source or a white LED. The devices with fluorescent lamps needed 30 min to stabilize to a constant radiant flux. In this study only five devices were measured. Radiant flux is just the first step to identify uniform specifications for light therapy devices. It is recommended to provide all five α-opic radiant fluxes. Preferably, the devices should come with a spectral power distribution of the radiant flux. For the devices equipped with a fluorescent lamp it is recommended to provide information on the stabilization time. Copyright © 2018 Elsevier B.V. All rights reserved.

Food-Growing, Air- And Water-Cleaning Module

NASA Technical Reports Server (NTRS)

Sauer, R. L.; Scheld, H. W.; Mafnuson, J. W.

1988-01-01

Apparatus produces fresh vegetables and removes pollutants from air. Hydroponic apparatus performs dual function of growing fresh vegetables and purifying air and water. Leafy vegetables rooted in granular growth medium grow in light of fluorescent lamps. Air flowing over leaves supplies carbon dioxide and receives fresh oxygen from them. Adaptable to production of food and cleaning of air and water in closed environments as in underwater research stations and submarines.

Fluorescent Lamp Replacement Study

DTIC Science & Technology

2017-07-01

DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 The public reporting burden for this collection of information is estimated to average 1 hour per...information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ORGANIZATION. 1. REPORT DATE (DD-MM...Unclassified 19b. TELEPHONE NUMBER (Include area code) Standard Form 298 (Rev. 8/98) Prescribed by ANSI Std. Z39.18 TABLE OF CONTENTS

Bis-ureidoquinoline as a selective fluoride anion sensor through hydrogen-bond interactions.

PubMed

Jo, Yunhee; Chidalla, Nagesh; Cho, Dong-Gyu

2014-10-03

Bis-ureidoquinoline shows a characteristic UV-vis absorbance and turn-on fluorescence changes in the presence of the fluoride anion. Such selective changes probably originate from the hydrogen-bond interactions, as shown by the (1)H NMR titration and DFT calculations. Bis-ureidoquinoline can be used as a fluoride-selective sensor for the detection of fluoride anions under illumination from a laboratory hand-held UV lamp.

Removal of mercury bonded in residual glass from spent fluorescent lamps.

PubMed

Rey-Raap, Natalia; Gallardo, Antonio

2013-01-30

The current technologies available for recycling fluorescent lamps do not completely remove the phosphor powder attached to the surface of the glass. Consequently, the glass contains the mercury diffused through the glass matrix and the mercury deposited in the phosphor powder that has not been removed during treatment at the recycling plant. A low-cost process, with just one stage, which can be used to remove the layer of phosphor powder attached to the surface of the glass and its mercury was studied. Several stirring tests were performed with different extraction mixtures, different liquid-solid ratios, and different agitation times. The value of the initial mercury concentration of the residual glass was 2.37 ± 0.50 μg/g. The maximum extraction percentage was 68.38%, obtained by stirring for 24 h with a liquid-solid ratio of 10 and using an extraction solution with 5% of an acid mixture prepared with HCl and HNO(3) at a ratio of 3:1 by volume. On an industrial scale the contact time could be reduced to 8 h without significantly lowering the percentage of mercury extracted. In fact, 64% of the mercury was extracted. Copyright © 2012 Elsevier Ltd. All rights reserved.

Effects of different light intensities in the morning on dim light melatonin onset.

PubMed

Kozaki, Tomoaki; Toda, Naohiro; Noguchi, Hiroki; Yasukouchi, Akira

2011-01-01

The present study evaluated the effects of exposure to light intensity in the morning on dim light melatonin onset (DLMO). The tested light intensities were 750 lux, 150 lux, 3000 lux, 6000 lux and 12,000 lux (horizontal illuminance at cornea), using commercial 5000 K fluorescent lamps. Eleven healthy males aged 21-31 participated in 2-day experiments for each light condition. On the first experimental day (day 1), subjects were exposed to dim light (<30 lux) for 3 h in the morning (09:00-12:00). On the same day, saliva samples were taken in dim light (<30 lux) every 30 min from 21:00 to 01:00 to determine the DLMO phase. The subjects were allowed to sleep from 01:00 to 08:00. On the second experimental day (day 2), the subjects were exposed to experimental light conditions for 3 h in the morning. The experimental schedule after light exposure was the same as on day 1. On comparing day 2 with day 1, significant phase advances of DLMO were obtained at 3000 lux, 6000 lux and 12,000 lux. These findings indicate that exposure to a necessary intensity from an ordinary light source, such as a fluorescent lamp, in the morning within one day affects melatonin secretion.

Caulobacter crescentus as a Whole-Cell Uranium Biosensor▿ †

PubMed Central

Hillson, Nathan J.; Hu, Ping; Andersen, Gary L.; Shapiro, Lucy

2007-01-01

We engineered a strain of the bacterium Caulobacter crescentus to fluoresce in the presence of micromolar levels of uranium at ambient temperatures when it is exposed to a hand-held UV lamp. Previous microarray experiments revealed that several Caulobacter genes are significantly upregulated in response to uranium but not in response to other heavy metals. We designated one of these genes urcA (for uranium response in caulobacter). We constructed a reporter that utilizes the urcA promoter to produce a UV-excitable green fluorescent protein in the presence of the uranyl cation, a soluble form of uranium. This reporter is specific for uranium and has little cross specificity for nitrate (<400 μM), lead (<150 μM), cadmium (<48 μM), or chromium (<41.6 μM). The uranium reporter construct was effective for discriminating contaminated groundwater samples (4.2 μM uranium) from uncontaminated groundwater samples (<0.1 μM uranium) collected at the Oak Ridge Field Research Center. In contrast to other uranium detection methodologies, the Caulobacter reporter strain can provide on-demand usability in the field; it requires minimal sample processing and no equipment other than a hand-held UV lamp, and it may be sprayed directly on soil, groundwater, or industrial surfaces. PMID:17905881

A retrospective analysis of compact fluorescent lamp experience curves and their correlations to deployment programs

DOE PAGES

Smith, Sarah Josephine; Wei, Max; Sohn, Michael D.

2016-09-17

Experience curves are useful for understanding technology development and can aid in the design and analysis of market transformation programs. Here, we employ a novel approach to create experience curves, to examine both global and North American compact fluorescent lamp (CFL) data for the years 1990–2007. We move away from the prevailing method of fitting a single, constant, exponential curve to data and instead search for break points where changes in the learning rate may have occurred. Our analysis suggests a learning rate of approximately 21% for the period of 1990–1997, and 51% and 79% in global and North Americanmore » datasets, respectively, after 1998. We use price data for this analysis; therefore our learning rates encompass developments beyond typical “learning by doing”, including supply chain impacts such as market competition. We examine correlations between North American learning rates and the initiation of new programs, abrupt technological advances, and economic and political events, and find an increased learning rate associated with design advancements and federal standards programs. Our findings support the use of segmented experience curves for retrospective and prospective technology analysis, and may imply that investments in technology programs have contributed to an increase of the CFL learning rate.« less

A retrospective analysis of compact fluorescent lamp experience curves and their correlations to deployment programs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, Sarah Josephine; Wei, Max; Sohn, Michael D.

Experience curves are useful for understanding technology development and can aid in the design and analysis of market transformation programs. Here, we employ a novel approach to create experience curves, to examine both global and North American compact fluorescent lamp (CFL) data for the years 1990–2007. We move away from the prevailing method of fitting a single, constant, exponential curve to data and instead search for break points where changes in the learning rate may have occurred. Our analysis suggests a learning rate of approximately 21% for the period of 1990–1997, and 51% and 79% in global and North Americanmore » datasets, respectively, after 1998. We use price data for this analysis; therefore our learning rates encompass developments beyond typical “learning by doing”, including supply chain impacts such as market competition. We examine correlations between North American learning rates and the initiation of new programs, abrupt technological advances, and economic and political events, and find an increased learning rate associated with design advancements and federal standards programs. Our findings support the use of segmented experience curves for retrospective and prospective technology analysis, and may imply that investments in technology programs have contributed to an increase of the CFL learning rate.« less

Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening.

PubMed

Choi, Gihoon; Prince, Theodore; Miao, Jun; Cui, Liwang; Guan, Weihua

2018-05-19

The effectiveness of malaria screening and treatment highly depends on the low-cost access to the highly sensitive and specific malaria test. We report a real-time fluorescence nucleic acid testing device for malaria field detection with automated and scalable sample preparation capability. The device consists a compact analyzer and a disposable microfluidic reagent compact disc. The parasite DNA sample preparation and subsequent real-time LAMP detection were seamlessly integrated on a single microfluidic compact disc, driven by energy efficient non-centrifuge based magnetic field interactions. Each disc contains four parallel testing units which could be configured either as four identical tests or as four species-specific tests. When configured as species-specific tests, it could identify two of the most life-threatening malaria species (P. falciparum and P. vivax). The NAT device is capable of processing four samples simultaneously within 50 min turnaround time. It achieves a detection limit of ~0.5 parasites/µl for whole blood, sufficient for detecting asymptomatic parasite carriers. The combination of the sensitivity, specificity, cost, and scalable sample preparation suggests the real-time fluorescence LAMP device could be particularly useful for malaria screening in the field settings. Copyright © 2018 Elsevier B.V. All rights reserved.

A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

PubMed Central

Priye, Aashish; Bird, Sara W.; Light, Yooli K.; Ball, Cameron S.; Negrete, Oscar A.; Meagher, Robert J.

2017-01-01

Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. Here we demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. Our smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Together, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most. PMID:28317856

A Highly Active System for the Metal-Free Aerobic Photocyanation of Tertiary Amines with Visible Light: Application to the Synthesis of Tetraponerines and Crispine A.

PubMed

Orejarena Pacheco, Julio Cesar; Lipp, Alexander; Nauth, Alexander M; Acke, Fabian; Dietz, Jule-Philipp; Opatz, Till

2016-04-04

A highly efficient metal-free catalytic system for the aerobic photocyanation of tertiary amines with visible light is reported. The use of air as terminal oxidant offers an improved safety profile compared with pure oxygen, the used compact fluorescent lamp (CFL) light sources are highly economical, and no halogenated solvents are required. This system not only proves to be effective for a wide variety of trialkylamines, pharmaceuticals, and alkaloids but remarkably also allows the lowest catalyst loading (0.00001 mol% or 0.1 ppm) ever reported for an organic dye. Bruylants reactions and C-alkylation/decyanations were performed on the obtained α-aminonitriles to demonstrate the postfunctionalization of complex molecules. The catalytic system is furthermore applied in the short and effective syntheses of the alkaloids (±)-crispine A and the tetraponerines T7 and T8. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Comparison of auto-fluorescence and tetracycline fluorescence for guided bone surgery of medication-related osteonecrosis of the jaw: a randomized controlled feasibility study.

PubMed

Ristow, O; Otto, S; Geiß, C; Kehl, V; Berger, M; Troeltzsch, M; Koerdt, S; Hohlweg-Majert, B; Freudlsperger, C; Pautke, C

2017-02-01

Recent studies have indicated that bone shows auto-fluorescence under an appropriate fluorescence lamp. The aim of this preliminary study was to compare the success rates of the established tetracycline fluorescence-guided bone surgery with auto-fluorescence-guided bone surgery in the treatment of medication-related osteonecrosis of the jaw (MRONJ). Forty patients suffering from MRONJ were referred for surgical treatment and were divided randomly into two groups: auto-fluorescence (n=20) or tetracycline fluorescence (n=20) guided bone surgery. The primary endpoint was treatment success, defined as the absence of exposed bone at 8 weeks after surgery. Secondary outcomes assessed were mucosal integrity, signs of infection, pain, and loss of sensitivity; these were evaluated descriptively at 10 days, 8 weeks, 6 months, and 1 year after surgery. At 8 weeks postoperative, 18/20 patients (90%) in the auto-fluorescence group and 17/20 patients (85%) in the tetracycline fluorescence group showed mucosal integrity (P>0.05). At the last follow-up, 94% in the auto-fluorescence group and 89% in the tetracycline fluorescence group presented complete mucosal coverage with no exposed bone, infection, or pain (P>0.05). There was no significant difference between the two techniques for any of the secondary outcomes (P>0.05). The results of this preliminary study show that auto-fluorescence-guided bone surgery has comparable success rates to the established tetracycline fluorescence-guided bone surgery. Copyright © 2016 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

Investigation of Passive Filter for LED Lamp

NASA Astrophysics Data System (ADS)

Sarwono, Edi; Facta, Mochammad; Handoko, Susatyo

2017-04-01

Light Emitting Diode lamp or LED lamp is one of the energy saving lamps nowadays widely used by consumers. However, LED lamp has contained harmonics caused by the rectifier circuit inside the lamp. Harmonics cause a quality problem in power system. As the harmonics present in current or voltage, the waveforms are distorted. Harmonics can lead to overheating in magnetic core of electrical equipments. In this paper, several tests are carried out to investigate the harmonic content of voltage and currents, and also the level of light intensity of the two brands of LED lamps. Measurements in this study are conducted by using HIOKI Power Quality Analyzer 3197. The test results show that the total harmonic distortion or THD of voltage on various brands of LED lamps did not exceed 5% as in compliance to the limit of IEEE standard 519-1992. The largest harmonic voltage is 2.9%, while maximum harmonic current for tested brands of LED lamp is 170.6%. The use of low pass filter in the form of LC filter was proposed. Based on experimental results, the application of LC filter at input side of LED lamp has successfully reduced THD current in the range of 85%-88%.

Efficient, full-spectrum, long-lived, non-toxic microwave lamp for plant growth

NASA Technical Reports Server (NTRS)

Maclennan, Donald A.; Turner, Brian P.; Dolan, James T.; Ury, Michael G.; Gustafson, Paul

1994-01-01

Fusion Systems Corporation has developed a mercury-free, low infrared, efficient microwave lamp using a benign sulfur based fill optimized for visible light. Our literature search and discussions with researchers directed us to enhance the bulbs red output. We have demonstrated a photosynthetic efficacy of over 2 micro-moles per microwave joule which corresponds to over 1.3 micro-moles per joule at the power main. Recent work has shown we can make additional increases in overall system efficiency. During the next two years, we expect to demonstrate a system capable of producing more than 1.5 micro-moles/joule measured at the power main with significantly less IR than alternative lamp systems. We determined optimal plant growth light requirements via a literature search and researcher input. We surveyed candidate lamp fill materials to be used in combination with sulfur and explored several methods of increasing photosynthetic efficacy.

[Sequential degradation of p-cresol by photochemical and biological methods].

PubMed

Karetnikova, E A; Chaĭkovskaia, O N; Sokolova, I V; Nikitina, L I

2008-01-01

Sequential photo- and biodegradation of p-cresol was studied using a mercury lamp, as well as KrCl and XeCl excilamps. Preirradiation of p-cresol at a concentration of 10(-4) M did not affect the rate of its subsequent biodegradation. An increase in the concentration of p-cresol to 10(-3) M and in the duration preliminary UV irradiation inhibited subsequent biodegradation. Biodegradation of p-cresol was accompanied by the formation of a product with a fluorescence maximum at 365 nm (lambdaex 280 nm), and photodegradation yielded a compound fluorescing at 400 nm (lambdaex 330 nm). Sequential UV and biodegradation led to the appearance of bands in the fluorescence spectra that were ascribed to p-cresol and its photolysis products. It was shown that sequential use of biological and photochemical degradation results in degradation of not only the initial toxicant but also the metabolites formed during its biodegradation.

Dimming of metal halide lamps

NASA Astrophysics Data System (ADS)

Schurer, Kees

1994-03-01

We ran some tests on the effect of dimming of metal halide (MH) lamps upon the stability and the spectral quality of the light output. Lamps used were a new Philips lamp HPI-T 250W, a similar Philips lamp with a few thousand burning hours and a new Osram lamp HQI-T 250W/D. The ballast was a BBC type DJ 250/2KS, the starter a BAS TORGI type MZN 250 SE and the dimmer an Elstrom Control System type ERHQ-T 250. Power was derived from a Philips stabilizer, type PE 1602. Lamp output was monitored with a PAR meter. Spectra were taken at 100% and at 50% output as measured with the PAR meter. Lamps were allowed to stabilize at any setting for 30 minutes before measurements were made. Lamp manufacturers advise against dimming for fear of poor stability and intolerable changes of the spectrum. However, none of the lamps showed a decrease in stability, no flicker or wandering of the discharge, and the changes of the spectrum were not negligible, but certainly not dramatic. Lamps of either manufacture retain their white color, relative peak heights of spectral lines did shift, but no gaps in the spectrum occurred. Spectra taken at 50% with 30 minutes intervals coincided. Differences between the new and the older Philips lamp were noticeable, but not really significant.

Dimming of metal halide lamps

NASA Technical Reports Server (NTRS)

Schurer, Kees

1994-01-01

We ran some tests on the effect of dimming of metal halide (MH) lamps upon the stability and the spectral quality of the light output. Lamps used were a new Philips lamp HPI-T 250W, a similar Philips lamp with a few thousand burning hours and a new Osram lamp HQI-T 250W/D. The ballast was a BBC type DJ 250/2KS, the starter a BAS TORGI type MZN 250 SE and the dimmer an Elstrom Control System type ERHQ-T 250. Power was derived from a Philips stabilizer, type PE 1602. Lamp output was monitored with a PAR meter. Spectra were taken at 100% and at 50% output as measured with the PAR meter. Lamps were allowed to stabilize at any setting for 30 minutes before measurements were made. Lamp manufacturers advise against dimming for fear of poor stability and intolerable changes of the spectrum. However, none of the lamps showed a decrease in stability, no flicker or wandering of the discharge, and the changes of the spectrum were not negligible, but certainly not dramatic. Lamps of either manufacture retain their white color, relative peak heights of spectral lines did shift, but no gaps in the spectrum occurred. Spectra taken at 50% with 30 minutes intervals coincided. Differences between the new and the older Philips lamp were noticeable, but not really significant.

LED lamp power management system and method

DOEpatents

Gaines, James; Clauberg, Bernd; Van Erp, Josephus A. M.

2013-03-19

An LED lamp power management system and method including an LED lamp having an LED controller 58; a plurality of LED channels 60 operably connected to the LED controller 58, each of the plurality of LED channels 60 having a channel switch 62 in series with at least one shunted LED circuit 83, the shunted LED circuit 83 having a shunt switch 68 in parallel with an LED source 80. The LED controller 58 reduces power loss in one of the channel switch 62 and the shunt switch 68 when LED lamp electronics power loss (P.sub.loss) exceeds an LED lamp electronics power loss limit (P.sub.lim); and each of the channel switches 62 receives a channel switch control signal 63 from the LED controller 58 and each of the shunt switches 68 receives a shunt switch control signal 69 from the LED controller 58.

Xenon lighting adjusted to plant requirements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koefferlein, M.; Doehring, T.; Payer, H.D.

1994-12-31

The high luminous flux and spectral properties of xenon lamps would provide an ideal luminary for plant lighting if not excess IR radiation poses several problems for an application: the required filter systems reduce the irradiance at spectral regions of particular importance for plant development. Most of the economical drawbacks of xenon lamps are related to the difficult handling of that excess IR energy. Furthermore, the temporal variation of the xenon output depending on the oscillations of the applied AC voltage has to be considered for the plant development. However, xenon lamps outperform other lighting systems with respect to spectralmore » stability, immediate response, and maximum luminance. Therefore, despite considerable competition by other lighting techniques, xenon lamps provide a very useful tool for special purposes. In plant lighting however, they seem to play a less important role as other lamp and lighting developments can meet these particular requirements at lower costs.« less

Molecular switch-modulated fluorescent copper nanoclusters for selective and sensitive detection of histidine and cysteine.

PubMed

Gu, Zefeng; Cao, Zhijuan

2018-06-07

A novel assay for histidine and cysteine has been constructed based on modulation of fluorescent copper nanoclusters (CuNCs) by molecular switches. In our previous work, a dumbbell DNA template with a poly-T (thymine) loop has been developed as an excellent template for the formation of strongly fluorescent CuNCs. Herein, for the first time, we established this biosensor for sensing two amino acids by using dumbbell DNA-templated CuNCs as the single probe. Among 20 natural amino acids, only histidine and cysteine can selectively quench fluorescence emission of CuNCs, because of the specific interaction of these compounds with copper ions. Furthermore, by using nickel ions (Ni 2+ ) and N-ethylmaleimide as the masking agents for histidine and cysteine respectively, an integrated logic gate system was designed by coupling with the fluorescent CuNCs and demonstrated selective and sensitive detection of cysteine and histidine. Under optimal conditions, cysteine can be detected in the concentration ranges of 0.01-10.0 μM with the detection limit (DL) of as low as 98 pM, while histidine can be detected in the ranges of 0.05-40.0 μM with DL of 1.6 nM. In addition, histidine and cysteine can be observed with the naked eye under a hand-held UV lamp (DL, 50 nM), which can be easily adapted to automated high-throughput screening. Finally, the strategy has been successfully utilized for biological fluids. The proposed system can be conducted in homogeneous solution, eliminating the need for organic cosolvents, separation processes of nanomaterials, or any chemical modifications. Overall, the assay provides an alternative method for simultaneous detection of cysteine and histidine by taking the advantages of high speed, no label and enzyme requirement, and good sensitivity and specificity, and will satisfy the great demand for determination of amino acids in fields such as food processing, biochemistry, pharmaceuticals, and clinical analysis. Graphical abstract.

Use of fluorescence and scanning electron microscopy as tools in teaching biology

NASA Astrophysics Data System (ADS)

Ghosh, Nabarun; Silva, Jessica; Vazquez, Aracely; Das, A. B.; Smith, Don W.

2011-06-01

Recent nationwide surveys reveal significant decline in students' interest in Math and Sciences. The objective of this project was to inspire young minds in using various techniques involved in Sciences including Scanning Electron Microscopy. We used Scanning Electron Microscope in demonstrating various types of Biological samples. An SEM Tabletop model in the past decade has revolutionized the use of Scanning Electron Microscopes. Using SEM Tabletop model TM 1000 we studied biological specimens of fungal spores, pollen grains, diatoms, plant fibers, dust mites, insect parts and leaf surfaces. We also used fluorescence microscopy to view, to record and analyze various specimens with an Olympus BX40 microscope equipped with FITC and TRITC fluorescent filters, a mercury lamp source, DP-70 digital camera with Image Pro 6.0 software. Micrographs were captured using bright field microscopy, the fluoresceinisothiocyanate (FITC) filter, and the tetramethylrhodamine (TRITC) filter settings at 40X. A high pressure mercury lamp or UV source was used to excite the storage molecules or proteins which exhibited autofluorescence. We used fluorescent microscopy to confirm the localization of sugar beet viruses in plant organs by viewing the vascular bundles in the thin sections of the leaves and other tissues. We worked with the REU summer students on sample preparation and observation on various samples utilizing the SEM. Critical Point Drying (CPD) and metal coating with the sputter coater was followed before observing some cultured specimen and the samples that were soft in textures with high water content. SEM Top allowed investigating the detailed morphological features that can be used for classroom teaching. Undergraduate and graduate researchers studied biological samples of Arthropods, pollen grains and teeth collected from four species of snakes using SEM. This project inspired the research students to pursue their career in higher studies in science and 45% of the undergraduates participated in this project entered Graduate school.

Experimental radiative lifetimes, branching fractions, and oscillator strengths of some levels in Tm III

NASA Astrophysics Data System (ADS)

Yu, Qi; Wang, Xinghao; Li, Qiu; Gong, Yimin; Dai, Zhenwen

2018-06-01

Natural radiative lifetimes for five even-parity levels of Tm III were measured by time-resolved laser-induced fluorescence method. The branching fraction measurements were performed based on the emission spectra of a hollow cathode lamp. By combining the measured branching fractions and the lifetime values reported in this work and in literature, experimental transition probabilities and oscillator strengths for 11 transitions were derived for the first time.

Abbreviated Environmental Assessment for the Northwest Infrastructure, Phase II Spangdahlem Air Base, Germany

DTIC Science & Technology

2007-12-01

Impact to flying insect species could be generated by artificial lightning. 2.1.5.2.3 Biotope connecting travel corridors would be impacted by the...well as applying lamps with light that is less attractive to night- active insects (e.g. gaslight, fluorescent tube). Mitigation measures for the...Devi l~bit (Scabious) symphytum officinale agg. Gew6hnlichcr Beinwcll Common Comfrey urtica dioica GroJ3e Brennesscl Stinging Nettle

Human Factors for Flight Deck Certification Personnel

DTIC Science & Technology

1993-07-01

Figure 2.2 Relative energy of fluorescent lamps plotted as a function of wavelength: 1 = standard warm white, 2 = white, 3 = standard cool white, 4...amounts of energy to be loud enough to be heard. it is interesting to consider our absolute sensitivity under optimal conditions. At about 2,500 Hz... airports have hearing losses that are correlated with the amount of time on the job (Taylor, 1965). Similar studies have shown deleterious effects of

Performance of a novel LED lamp arrangement to reduce SNR fluctuation for multi-user visible light communication systems.

PubMed

Wang, Zixiong; Yu, Changyuan; Zhong, Wen-De; Chen, Jian; Chen, Wei

2012-02-13

This paper investigates the performance of our recently proposed LED lamp arrangement to reduce the SNR fluctuation from different locations in the room for multi-user visible light communications. The LED lamp arrangement consists of 4 LED lamps positioned in the corners and 12 LED lamps spread evenly on a circle. Our studies show that the SNR fluctuation under such a LED lamp arrangement is reduced from 14.5 dB to 0.9 dB, which guarantees that users can obtain almost identical communication quality, regardless of their locations. After time domain zero-forcing (ZF) equalization, the BER performances and channel capacities of 100-Mbit/s and 200-Mbit/s bipolar on-off-keying (OOK) signal with most significant inter-symbol interference (ISI) are very close to that of the channel without any ISI caused by this LED lamp arrangement.

Investigations of HID Lamp Electrodes under HF Operation

NASA Astrophysics Data System (ADS)

Reinelt, Jens; Langenscheidt, Oliver; Westermeier, Michael; Mentel, Juergen; Awakowicz, Peter

2007-10-01

Low pressure lamps are operated many years at high frequencies to improve the efficiency of these lamps and drivers. For high pressure discharge lamps this operation mode has not been installed yet. Generally it can be assumed that there are changes in the electrode physics which may lead to an undesired lamp behavior if HID lamps are operated at a high frequency. To gain insights into these fundamental changes the so called Bochum Model Lamp is used. It is an easy system which allows a fundamental research on HID electrode behavior and the near electrode region without the occurrence of acoustic resonances. For the investigation phase resolved photography, pyrometry and spectrometry is used. The presented results describe changes in the electrode temperature and changes in the kind of arc attachment on the electrodes (diffuse and spot mode) depending on frequency. Also measurements of the Electrode-Sheath-Voltage (ESV), depending on frequency, are presented.

76 FR 44506 - Petition Requesting Non-See-Through Packaging for Torch Fuel and Lamp Oil

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-26

... New Jersey Poison Information and Education System stated in June 2008 that four people were... Control Centers' National Data System has chronicled the exposure of many young children to lamp oils...

75 FR 67634 - Parts and Accessories Necessary for Safe Operation: Antilock Brake Systems

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-03

... trailer with an antilock brake system be equipped with an external malfunction indicator lamp. DATES: This... malfunction indicator lamp. FMCSA used the Agency's direct final rule procedures (75 FR 29915, May 28, 2010...

A new Cassegrain calibration lamp unit for the Blanco Telescope

NASA Astrophysics Data System (ADS)

Points, S. D.; James, D. J.; Tighe, R.; Montané, A.; David, N.; Martínez, M.

2016-08-01

The f/8 RC-Cassegrain Focus of the Blanco Telescope at Cerro Tololo Inter-American Observatory, hosts two new instruments: COSMOS, a multi-object spectrograph in the visible wavelength range (350 - 1030nm), and ARCoIRIS, a NIR cross-dispersed spectrograph featuring 6 spectral orders spanning 0.8 - 2.45μm. Here we describe a calibration lamp unit designed to deliver the required illumination at the telescope focal plane for both instruments. These requirements are: (1) an f/8 beam of light covering a spot of 92mm diameter (or 10 arcmin) for a wavelength range of 0.35μm through 2.5μm and (2) no saturation of flat-field calibrations for the minimal exposure times permitted by each instrument, and (3) few saturated spectral lines when using the wavelength calibration lamps for the instruments. To meet these requirements this unit contains an adjustable quartz halogen lamp for flat-field calibrations, and one hollow cathode lamp and four penray lamps for wavelength calibrations. The wavelength calibration lamps are selected to provide optimal spectral coverage for the instrument mounted and can be used individually or in sets. The device designed is based on an 8-inch diameter integrating sphere, the output of which is optimized to match the f/8 calibration input delivery system which is a refractive system based on fused-silica lenses. We describe the optical design, the opto-mechanical design, the electronic control and give results of the performance of the system.

Artificial light sources for simulating natural daylight and skylight.

PubMed

Grum, F

1968-01-01

A review of the literature reveals the need for reliable and stable artificial light sources that can be used as simulators of daylight and skylight. In quest of such simulators a first requirement is quantitative information on the average spectral distributions of natural sources such as daylight and skylight. Recent investigations of the spectral energy characteristics of natural daylight and skylight made it possible to determine such average conditions. With these conditions established, a search was undertaken for an artificial light source that would simulate these average natural distributions with a minimum of filtering. Certain fluorescent lamps and combinations of them were considered first, but, although it was possible to achieve fairly good visual matches of daylight and skylight, the spectral characteristics and the variability of such combinations are drawbacks to their use in critical scientific work. For this purpose, therefore, xenon arc lamps were found to be superior.

Options for reducing carbon dioxide emissions

NASA Astrophysics Data System (ADS)

Rosenfeld, Arthur H.; Price, Lynn

1992-03-01

Improvements in energy efficiency can significantly reduce the annual growth in greenhouse gas emissions. Such improvements occur when energy intensity is reduced; no reduction in energy services is required. Using the concept of ``cost of conserved energy'' to develop conservation supply curves similar to resource supply curves, researchers consistently find that electricity and natural gas savings of nearly 50% of current consumption are possible for U.S. buildings. Such reductions in energy consumption directly reduce emissions of greenhouse gases. To capture these savings, we must continue to develop energy-efficient technologies and strategies. This paper describes three recent energy-efficient technologies that benefitted from energy conservation research and development (R&D) funding: high-frequency ballasts, compact fluorescent lamps, and low-emissivity windows. Other advanced technologies and strategies of spectrally selective windows, superwindows, electrochromic windows, advanced insulation, low-flow showerheads, improved recessed lamp fixtures, whitening surfaces and planting urban trees, daylighting, and thermal energy storage are also discussed.

[Functional state of the visual analyzer in the conditions of the use of traditional and LED light sources].

PubMed

Kaptsov, V A; Sosunov, N N; Shishchenko, I I; Viktorov, V S; Tulushev, V N; Deynego, V N; Bukhareva, E A; Murashova, M A; Shishchenko, A A

2014-01-01

There was performed the experimental work on the study of the possibility of the application of LED lighting (LED light sources) in rail transport for traffic safety in related professions. Results of 4 series of studies involving 10 volunteers for the study and a comparative evaluation of the functional state of the visual analyzer, the general functional state and mental capacity under the performing the simulated operator activity in conditions of traditional light sources (incandescent, fluorescent lamp) and the new LED (LED lamp, LED panel) light sources have revealed changes in the negative direction. This was pronounced in a some decrease of functional stability to color discrimination between green and red cone signals, as well as an increase in response time in complex visual--motor response and significant reduction in readiness for emergency action of examinees.

Automated system for on-line determination of dimethylarsinic and inorganic arsenic by hydride generation-atomic fluorescence spectrometry.

PubMed

Chaparro, L L; Ferrer, L; Cerdà, V; Leal, L O

2012-09-01

A multisyringe flow-injection approach has been coupled to hydride generation-atomic fluorescence spectrometry (HG-AFS) with UV photo-oxidation for dimethylarsinic (DMA), inorganic As and total As determination, depending on the pre-treatment given to the sample (extraction or digestion). The implementation of a UV lamp allows on-line photo-oxidation of DMA and the following arsenic detection, whereas a bypass leads the flow directly to the HG-AFS system, performing inorganic arsenic determination. DMA concentration is calculated by the difference of total inorganic arsenic and measurement of the photo-oxidation step. The detection limits for DMA and inorganic arsenic were 0.09 and 0.47 μg L(-1), respectively. The repeatability values accomplished were of 2.4 and 1.8%, whereas the injection frequencies were 24 and 28 injections per hour for DMA and inorganic arsenic, respectively. This method was validated by means of a solid reference material BCR-627 (muscle of tuna) with good agreement with the certified values. Satisfactory results for DMA and inorganic arsenic determination were obtained in several water matrices. The proposed method offers several advantages, such as increasing the sampling frequency, low detection limits and decreasing reagents and sample consumption, which leads to lower waste generation.

Laser induced fluorescence spectroscopy used for the investigation of Landé gJ- factors of praseodymium energy levels

NASA Astrophysics Data System (ADS)

Sobolewski, Ł. M.; Windholz, L.; Kwela, J.

2017-06-01

Laser induced fluorescence (LIF) spectroscopy was used for the investigation of structures of 52 spectral lines of Pr I in the wavelength range 561.3 - 613.9 nm. As a source of free Pr atoms a hollow cathode discharge lamp was used. We monitored selected LIF signals appearing when the laser beam excites the hollow cathode plasma. LIF spectra were recorded in the presence of a magnetic field of about 800 G produced by a permanent magnet for two linear polarizations of the exciting laser beam. We have determined for the first time Landé gJ- factors for 71 levels of neutral Pr and reinvestigated data for several other levels.

[Study on the discharge properties of xeon flash lamp and experimental measurement].

PubMed

Zhao, You-Quan; Miao, Pei-Liang; He, Feng; Gu, Jian; Zhai, Rui-Wei

2014-07-01

The Xenon flash lamp is a new type of light source for analytical instrument. The present paper analyzed the discharge process of xenon flash lamp, presented the discharge test system, and conducted experimental measurement of the voltage, current and optical pulse signal in the process of discharge. The results show that in the preliminary discharge, the free electron concentration was at a low level, so the energy was at a low level, then following the gas discharge, numerous free electrons formed in the lamp, resultin in the increase in the concentration of free electrons, therefore discharge current rised rapidly and voltage reduced. The lamp released photons to generate light pulse in the moment of ionic recombination, The pulse xenon lamp light energy output and spectral characteristic is related to electron energy in recombination and combination level of xenon, if the input energy and the energy consumption of the xenon lamp is inconsistent, it will lead to repeated capacitor charging and discharging and produce oscillation waveform. This paper is very useful for understanding the process of xenon lamp discharge, optimizing the driver circuit and the production of xenon flash lamp.

Handheld dual fluorescence and reflection spectroscopy system for monitoring topical low dose ALA-PDT of actinic keratoses (AK)

NASA Astrophysics Data System (ADS)

Charamisinau, Ivan; Keymel, Kenneth; Potter, William; Oseroff, Allan R.

2006-02-01

Photodynamic therapy is an effective, minimally invasive skin cancer treatment modality with few side effects. Improved therapeutic selectivity and efficacy is expected if treatment is optimized individually for each patient based on detailed measurements prior and during the treatment. The handheld system presented allows measuring optical properties of the skin, the rate of photosensitizer photobleaching during the ALA PDT and oxygen saturation in the tissue. The photobleaching rate is monitored using fluorescence spectroscopy, where protoporphyrin IX in tissue is exited by 410 nm (blue) or 532 nm (green) laser light, and fluorescence in the 580-800 nm range is monitored. The photobleaching rate is calculated by correlating the measured spectrum with known protoporphyrin IX, photoproduct and nonspecific tissue autofluorescence spectra using correlation analysis. Double-wavelength excitation allows a rough estimation of the depth of the fluorescence source due to the significant difference in penetration depth for blue and green light. Blood concentration and oxygenation in the tissue are found from the white light reflectance spectrum in the 460-800 nm range. Known spectra for the oxy- and deoxyhemoglobin, melanin, and tissue baseline absorption and tissue scattering are substituted in nonlinear equations to find the penetration depth and diffuse reflectance coefficient. The nonlinear equation for the diffuse reflectance coefficient is solved for blood and melanin concentrations and blood oxygenation values that provide the best fit to the measured spectrum. The optical properties of the tissue obtained from the reflectance spectroscopy are used to correct the fluorescence data. A noncontact probe with 5 fibers (3 excitation and 2 detection) focused to the same 5 mm diameter spot: 2 excitation lasers, a white light lamp and a two-channel spectrometer are used. A LabView program with custom nonlinear equation solvers written in C++ automatically performs the measurements and calculations, and writes data to a database. The system is currently used in a clinical trial to find the relationship between skin pigmentation, oxygen saturation in blood, photobleaching rate and optimal fluence rate for skin cancer treatment of actinic keratoses.

Numerical and experimental analyses of the radiant heat flux produced by quartz heating systems

NASA Technical Reports Server (NTRS)

Turner, Travis L.; Ash, Robert L.

1994-01-01

A method is developed for predicting the radiant heat flux distribution produced by tungsten filament, tubular fused-quartz envelope heating systems with reflectors. The method is an application of Monte Carlo simulation, which takes the form of a random walk or ray tracing scheme. The method is applied to four systems of increasing complexity, including a single lamp without a reflector, a single lamp with a Hat reflector, a single lamp with a parabolic reflector, and up to six lamps in a six-lamp contoured-reflector heating unit. The application of the Monte Carlo method to the simulation of the thermal radiation generated by these systems is discussed. The procedures for numerical implementation are also presented. Experiments were conducted to study these quartz heating systems and to acquire measurements of the corresponding empirical heat flux distributions for correlation with analysis. The experiments were conducted such that several complicating factors could be isolated and studied sequentially. Comparisons of the experimental results with analysis are presented and discussed. Good agreement between the experimental and simulated results was obtained in all cases. This study shows that this method can be used to analyze very complicated quartz heating systems and can account for factors such as spectral properties, specular reflection from curved surfaces, source enhancement due to reflectors and/or adjacent sources, and interaction with a participating medium in a straightforward manner.

Intraocular light scatter, reflections, fluorescence and absorption: what we see in the slit lamp.

PubMed

van den Berg, Thomas J T P

2018-01-01

Much knowledge has been collected over the past 20 years about light scattering in the eye- in particular in the eye lens- and its visual effect, called straylight. It is the purpose of this review to discuss how these insights can be applied to understanding the slit lamp image. The slit lamp image mainly results from back scattering, whereas the effects on vision result mainly from forward scatter. Forward scatter originates from particles of about wavelength size distributed throughout the lens. Most of the slit lamp image originates from small particle scatter (Rayleigh scatter). For a population of middle aged lenses it will be shown that both these scatter components remove around 10% of the light from the direct beam. For slit lamp observation close to the reflection angles, zones of discontinuity (Wasserspalten) at anterior and posterior parts of the lens show up as rough surface reflections. All these light scatter effects increase with age, but the correlations with age, and also between the different components, are weak. For retro-illumination imaging it will be argued that the density or opacity seen in areas of cortical or posterior subcapsular cataract show up because of light scattering, not because of light loss. NOTES: (1) Light scatter must not be confused with aberrations. Light penetrating the eye is divided into two parts: a relatively small part is scattered, and removed from the direct beam. Most of the light is not scattered, but continues as the direct beam. This non-scattered part is the basis for functional imaging, but its quality is under the control of aberrations. Aberrations deflect light mainly over small angles (<1°), whereas light scatter is important because of the straylight effects over large angles (>1°), causing problems like glare and hazy vision. (2) The slit lamp image in older lenses and nuclear cataract is strongly influenced by absorption. However, this effect is greatly exaggerated by the light path lengths concerned. This obviates proper judgement of the functional importance of absorption, and hinders the appreciation of the Rayleigh nature of what is seen in the slit lamp image. © 2017 The Authors Ophthalmic & Physiological Optics © 2017 The College of Optometrists.

Property of lysosomal storage disease associated with midbrain pathology in the central nervous system of Lamp-2-deficient mice.

PubMed

Furuta, Akiko; Kikuchi, Hisae; Fujita, Hiromi; Yamada, Daisuke; Fujiwara, Yuuki; Kabuta, Tomohiro; Nishino, Ichizo; Wada, Keiji; Uchiyama, Yasuo

2015-06-01

Lysosome-associated membrane protein-2 (LAMP-2) is the gene responsible for Danon disease, which is characterized by cardiomyopathy, autophagic vacuolar myopathy, and variable mental retardation. To elucidate the function of LAMP-2 in the central nervous system, we investigated the neuropathological changes in Lamp-2-deficient mice. Immunohistochemical observations revealed that Lamp-1 and cathepsin D-positive lysosomal structures increased in the large neurons of the mouse brain. Ubiquitin-immunoreactive aggregates and concanavalin A-positive materials were detected in these neurons. By means of ultrastructural studies, we found various-shaped accumulations, including lipofuscin, glycolipid-like materials, and membranous structures, in the neurons and glial cells of Lamp-2-deficient brains. In deficient mice, glycogen granules accumulated in hepatocyte lysosomes but were not observed in neurons. These pathological features indicate lysosomal storage disease; however, the findings are unlikely a consequence of deficiency of a single lysosomal enzyme. Although previous study results have shown a large amount of autophagic vacuoles in parenchymal cells of the visceral organs, these findings were rarely detected in the brain tissue except for some axons in the substantia nigra, in which abundant activated microglial cells with increased lipid peroxidation were observed. Thus, LAMP-2 in the central nervous system has a possible role in the degradation of the various macromolecules in lysosomes and an additional function concerning protection from oxidative stress, especially in the substantia nigra. Copyright © 2015 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.