High-resolution remotely sensed small target detection by imitating fly visual perception mechanism.
Huang, Fengchen; Xu, Lizhong; Li, Min; Tang, Min
2012-01-01
The difficulty and limitation of small target detection methods for high-resolution remote sensing data have been a recent research hot spot. Inspired by the information capture and processing theory of fly visual system, this paper endeavors to construct a characterized model of information perception and make use of the advantages of fast and accurate small target detection under complex varied nature environment. The proposed model forms a theoretical basis of small target detection for high-resolution remote sensing data. After the comparison of prevailing simulation mechanism behind fly visual systems, we propose a fly-imitated visual system method of information processing for high-resolution remote sensing data. A small target detector and corresponding detection algorithm are designed by simulating the mechanism of information acquisition, compression, and fusion of fly visual system and the function of pool cell and the character of nonlinear self-adaption. Experiments verify the feasibility and rationality of the proposed small target detection model and fly-imitated visual perception method.
Fox, Jessica L.; Aptekar, Jacob W.; Zolotova, Nadezhda M.; Shoemaker, Patrick A.; Frye, Mark A.
2014-01-01
The behavioral algorithms and neural subsystems for visual figure–ground discrimination are not sufficiently described in any model system. The fly visual system shares structural and functional similarity with that of vertebrates and, like vertebrates, flies robustly track visual figures in the face of ground motion. This computation is crucial for animals that pursue salient objects under the high performance requirements imposed by flight behavior. Flies smoothly track small objects and use wide-field optic flow to maintain flight-stabilizing optomotor reflexes. The spatial and temporal properties of visual figure tracking and wide-field stabilization have been characterized in flies, but how the two systems interact spatially to allow flies to actively track figures against a moving ground has not. We took a systems identification approach in flying Drosophila and measured wing-steering responses to velocity impulses of figure and ground motion independently. We constructed a spatiotemporal action field (STAF) – the behavioral analog of a spatiotemporal receptive field – revealing how the behavioral impulse responses to figure tracking and concurrent ground stabilization vary for figure motion centered at each location across the visual azimuth. The figure tracking and ground stabilization STAFs show distinct spatial tuning and temporal dynamics, confirming the independence of the two systems. When the figure tracking system is activated by a narrow vertical bar moving within the frontal field of view, ground motion is essentially ignored despite comprising over 90% of the total visual input. PMID:24198267
Visual control of flight speed in Drosophila melanogaster.
Fry, Steven N; Rohrseitz, Nicola; Straw, Andrew D; Dickinson, Michael H
2009-04-01
Flight control in insects depends on self-induced image motion (optic flow), which the visual system must process to generate appropriate corrective steering maneuvers. Classic experiments in tethered insects applied rigorous system identification techniques for the analysis of turning reactions in the presence of rotating pattern stimuli delivered in open-loop. However, the functional relevance of these measurements for visual free-flight control remains equivocal due to the largely unknown effects of the highly constrained experimental conditions. To perform a systems analysis of the visual flight speed response under free-flight conditions, we implemented a 'one-parameter open-loop' paradigm using 'TrackFly' in a wind tunnel equipped with real-time tracking and virtual reality display technology. Upwind flying flies were stimulated with sine gratings of varying temporal and spatial frequencies, and the resulting speed responses were measured from the resulting flight speed reactions. To control flight speed, the visual system of the fruit fly extracts linear pattern velocity robustly over a broad range of spatio-temporal frequencies. The speed signal is used for a proportional control of flight speed within locomotor limits. The extraction of pattern velocity over a broad spatio-temporal frequency range may require more sophisticated motion processing mechanisms than those identified in flies so far. In Drosophila, the neuromotor pathways underlying flight speed control may be suitably explored by applying advanced genetic techniques, for which our data can serve as a baseline. Finally, the high-level control principles identified in the fly can be meaningfully transferred into a robotic context, such as for the robust and efficient control of autonomous flying micro air vehicles.
NASA Astrophysics Data System (ADS)
Guo, Dejun; Bourne, Joseph R.; Wang, Hesheng; Yim, Woosoon; Leang, Kam K.
2017-08-01
This paper presents the design and implementation of an adaptive-repetitive visual-servo control system for a moving high-flying vehicle (HFV) with an uncalibrated camera to monitor, track, and precisely control the movements of a low-flying vehicle (LFV) or mobile ground robot. Applications of this control strategy include the use of high-flying unmanned aerial vehicles (UAVs) with computer vision for monitoring, controlling, and coordinating the movements of lower altitude agents in areas, for example, where GPS signals may be unreliable or nonexistent. When deployed, a remote operator of the HFV defines the desired trajectory for the LFV in the HFV's camera frame. Due to the circular motion of the HFV, the resulting motion trajectory of the LFV in the image frame can be periodic in time, thus an adaptive-repetitive control system is exploited for regulation and/or trajectory tracking. The adaptive control law is able to handle uncertainties in the camera's intrinsic and extrinsic parameters. The design and stability analysis of the closed-loop control system is presented, where Lyapunov stability is shown. Simulation and experimental results are presented to demonstrate the effectiveness of the method for controlling the movement of a low-flying quadcopter, demonstrating the capabilities of the visual-servo control system for localization (i.e.,, motion capturing) and trajectory tracking control. In fact, results show that the LFV can be commanded to hover in place as well as track a user-defined flower-shaped closed trajectory, while the HFV and camera system circulates above with constant angular velocity. On average, the proposed adaptive-repetitive visual-servo control system reduces the average RMS tracking error by over 77% in the image plane and over 71% in the world frame compared to using just the adaptive visual-servo control law.
Visuomotor Transformation in the Fly Gaze Stabilization System
Huston, Stephen J; Krapp, Holger G
2008-01-01
For sensory signals to control an animal's behavior, they must first be transformed into a format appropriate for use by its motor systems. This fundamental problem is faced by all animals, including humans. Beyond simple reflexes, little is known about how such sensorimotor transformations take place. Here we describe how the outputs of a well-characterized population of fly visual interneurons, lobula plate tangential cells (LPTCs), are used by the animal's gaze-stabilizing neck motor system. The LPTCs respond to visual input arising from both self-rotations and translations of the fly. The neck motor system however is involved in gaze stabilization and thus mainly controls compensatory head rotations. We investigated how the neck motor system is able to selectively extract rotation information from the mixed responses of the LPTCs. We recorded extracellularly from fly neck motor neurons (NMNs) and mapped the directional preferences across their extended visual receptive fields. Our results suggest that—like the tangential cells—NMNs are tuned to panoramic retinal image shifts, or optic flow fields, which occur when the fly rotates about particular body axes. In many cases, tangential cells and motor neurons appear to be tuned to similar axes of rotation, resulting in a correlation between the coordinate systems the two neural populations employ. However, in contrast to the primarily monocular receptive fields of the tangential cells, most NMNs are sensitive to visual motion presented to either eye. This results in the NMNs being more selective for rotation than the LPTCs. Thus, the neck motor system increases its rotation selectivity by a comparatively simple mechanism: the integration of binocular visual motion information. PMID:18651791
Steering a virtual blowfly: simulation of visual pursuit.
Boeddeker, Norbert; Egelhaaf, Martin
2003-09-22
The behavioural repertoire of male flies includes visually guided chasing after moving targets. The visuomotor control system for these pursuits belongs to the fastest found in the animal kingdom. We simulated a virtual fly, to test whether or not experimentally established hypotheses on the underlying control system are sufficient to explain chasing behaviour. Two operating instructions for steering the chasing virtual fly were derived from behavioural experiments: (i) the retinal size of the target controls the fly's forward speed and, thus, indirectly its distance to the target; and (ii) a smooth pursuit system uses the retinal position of the target to regulate the fly's flight direction. Low-pass filters implement neuronal processing time. Treating the virtual fly as a point mass, its kinematics are modelled in consideration of the effects of translatory inertia and air friction. Despite its simplicity, the model shows behaviour similar to that of real flies. Depending on its starting position and orientation as well as on target size and speed, the virtual fly either catches the target or follows it indefinitely without capture. These two behavioural modes of the virtual fly emerge from the control system for flight steering without implementation of an explicit decision maker.
Santer, Roger D.
2014-01-01
Palpalis group tsetse flies are the major vectors of human African trypanosomiasis, and visually-attractive targets and traps are important tools for their control. Considerable efforts are underway to optimise these visual baits, and one factor that has been investigated is coloration. Analyses of the link between visual bait coloration and tsetse fly catches have used methods which poorly replicate sensory processing in the fly visual system, but doing so would allow the visual information driving tsetse attraction to these baits to be more fully understood, and the reflectance spectra of candidate visual baits to be more completely analysed. Following methods well established for other species, I reanalyse the numbers of tsetse flies caught at visual baits based upon the calculated photoreceptor excitations elicited by those baits. I do this for large sets of previously published data for Glossina fuscipes fuscipes (Lindh et al. (2012). PLoS Negl Trop Dis 6: e1661), G. palpalis palpalis (Green (1988). Bull Ent Res 78: 591), and G. pallidipes (Green and Flint (1986). Bull Ent Res 76: 409). Tsetse attraction to visual baits in these studies can be explained by a colour opponent mechanism to which the UV-blue photoreceptor R7y contributes positively, and both the green-yellow photoreceptor R8y, and the low-wavelength UV photoreceptor R7p, contribute negatively. A tool for calculating fly photoreceptor excitations is made available with this paper, and this will facilitate a complete and biologically authentic description of visual bait reflectance spectra that can be employed in the search for more efficacious visual baits, or the analysis of future studies of tsetse fly attraction. PMID:25473844
Santer, Roger D
2014-12-01
Palpalis group tsetse flies are the major vectors of human African trypanosomiasis, and visually-attractive targets and traps are important tools for their control. Considerable efforts are underway to optimise these visual baits, and one factor that has been investigated is coloration. Analyses of the link between visual bait coloration and tsetse fly catches have used methods which poorly replicate sensory processing in the fly visual system, but doing so would allow the visual information driving tsetse attraction to these baits to be more fully understood, and the reflectance spectra of candidate visual baits to be more completely analysed. Following methods well established for other species, I reanalyse the numbers of tsetse flies caught at visual baits based upon the calculated photoreceptor excitations elicited by those baits. I do this for large sets of previously published data for Glossina fuscipes fuscipes (Lindh et al. (2012). PLoS Negl Trop Dis 6: e1661), G. palpalis palpalis (Green (1988). Bull Ent Res 78: 591), and G. pallidipes (Green and Flint (1986). Bull Ent Res 76: 409). Tsetse attraction to visual baits in these studies can be explained by a colour opponent mechanism to which the UV-blue photoreceptor R7y contributes positively, and both the green-yellow photoreceptor R8y, and the low-wavelength UV photoreceptor R7p, contribute negatively. A tool for calculating fly photoreceptor excitations is made available with this paper, and this will facilitate a complete and biologically authentic description of visual bait reflectance spectra that can be employed in the search for more efficacious visual baits, or the analysis of future studies of tsetse fly attraction.
Ichida, J M; Rosa, M G; Casagrande, V A
2000-01-31
It has been proposed that flying foxes and echolocating bats evolved independently from early mammalian ancestors in such a way that flying foxes form one of the suborders most closely related to primates. A major piece of evidence offered in support of a flying fox-primate link is the highly developed visual system of flying foxes, which is theorized to be primate-like in several different ways. Because the calcium-binding proteins parvalbumin (PV) and calbindin (CB) show distinct and consistent distributions in the primate visual system, the distribution of these same proteins was examined in the flying fox (Pteropus poliocephalus) visual system. Standard immunocytochemical techniques reveal that PV labeling within the lateral geniculate nucleus (LGN) of the flying fox is sparse, with clearly labeled cells located only within layer 1, adjacent to the optic tract. CB labeling in the LGN is profuse, with cells labeled in all layers throughout the nucleus. Double labeling reveals that all PV+ cells also contain CB, and that these cells are among the largest in the LGN. In primary visual cortex (V1) PV and CB label different classes of non-pyramidal neurons. PV+ cells are found in all cortical layers, although labeled cells are found only rarely in layer I. CB+ cells are found primarily in layers II and III. The density of PV+ neuropil correlates with the density of cytochrome oxidase staining; however, no CO+ or PV+ or CB+ patches or blobs are found in V1. These results show that the distribution of calcium-binding proteins in the flying fox LGN is unlike that found in primates, in which antibodies for PV and CB label specific separate populations of relay cells that exist in different layers. Indeed, the pattern of calcium-binding protein distribution in the flying fox LGN is different from that reported in any other terrestrial mammal. Within V1 no PV+ patches, CO blobs, or patchy distribution of CB+ neuropil that might reveal interblobs characteristic of primate V1 are found; however, PV and CB are found in separate populations of non-pyramidal neurons. The types of V1 cells labeled with antibodies to PV and CB in all mammals examined including the flying fox suggest that the similarities in the cellular distribution of these proteins in cortex reflect the fact that this feature is common to all mammals.
Haltere mechanosensory influence on tethered flight behavior in Drosophila.
Mureli, Shwetha; Fox, Jessica L
2015-08-01
In flies, mechanosensory information from modified hindwings known as halteres is combined with visual information for wing-steering behavior. Haltere input is necessary for free flight, making it difficult to study the effects of haltere ablation under natural flight conditions. We thus used tethered Drosophila melanogaster flies to examine the relationship between halteres and the visual system, using wide-field motion or moving figures as visual stimuli. Haltere input was altered by surgically decreasing its mass, or by removing it entirely. Haltere removal does not affect the flies' ability to flap or steer their wings, but it does increase the temporal frequency at which they modify their wingbeat amplitude. Reducing the haltere mass decreases the optomotor reflex response to wide-field motion, and removing the haltere entirely does not further decrease the response. Decreasing the mass does not attenuate the response to figure motion, but removing the entire haltere does attenuate the response. When flies are allowed to control a visual stimulus in closed-loop conditions, haltereless flies fixate figures with the same acuity as intact flies, but cannot stabilize a wide-field stimulus as accurately as intact flies can. These manipulations suggest that the haltere mass is influential in wide-field stabilization, but less so in figure tracking. In both figure and wide-field experiments, we observe responses to visual motion with and without halteres, indicating that during tethered flight, intact halteres are not strictly necessary for visually guided wing-steering responses. However, the haltere feedback loop may operate in a context-dependent way to modulate responses to visual motion. © 2015. Published by The Company of Biologists Ltd.
Vision in Flies: Measuring the Attention Span
Koenig, Sebastian; Wolf, Reinhard; Heisenberg, Martin
2016-01-01
A visual stimulus at a particular location of the visual field may elicit a behavior while at the same time equally salient stimuli in other parts do not. This property of visual systems is known as selective visual attention (SVA). The animal is said to have a focus of attention (FoA) which it has shifted to a particular location. Visual attention normally involves an attention span at the location to which the FoA has been shifted. Here the attention span is measured in Drosophila. The fly is tethered and hence has its eyes fixed in space. It can shift its FoA internally. This shift is revealed using two simultaneous test stimuli with characteristic responses at their particular locations. In tethered flight a wild type fly keeps its FoA at a certain location for up to 4s. Flies with a mutation in the radish gene, that has been suggested to be involved in attention-like mechanisms, display a reduced attention span of only 1s. PMID:26848852
Vision in Flies: Measuring the Attention Span.
Koenig, Sebastian; Wolf, Reinhard; Heisenberg, Martin
2016-01-01
A visual stimulus at a particular location of the visual field may elicit a behavior while at the same time equally salient stimuli in other parts do not. This property of visual systems is known as selective visual attention (SVA). The animal is said to have a focus of attention (FoA) which it has shifted to a particular location. Visual attention normally involves an attention span at the location to which the FoA has been shifted. Here the attention span is measured in Drosophila. The fly is tethered and hence has its eyes fixed in space. It can shift its FoA internally. This shift is revealed using two simultaneous test stimuli with characteristic responses at their particular locations. In tethered flight a wild type fly keeps its FoA at a certain location for up to 4s. Flies with a mutation in the radish gene, that has been suggested to be involved in attention-like mechanisms, display a reduced attention span of only 1s.
A conditioned visual orientation requires the ellipsoid body in Drosophila
Guo, Chao; Du, Yifei; Yuan, Deliang; Li, Meixia; Gong, Haiyun; Gong, Zhefeng
2015-01-01
Orientation, the spatial organization of animal behavior, is an essential faculty of animals. Bacteria and lower animals such as insects exhibit taxis, innate orientation behavior, directly toward or away from a directional cue. Organisms can also orient themselves at a specific angle relative to the cues. In this study, using Drosophila as a model system, we established a visual orientation conditioning paradigm based on a flight simulator in which a stationary flying fly could control the rotation of a visual object. By coupling aversive heat shocks to a fly's orientation toward one side of the visual object, we found that the fly could be conditioned to orientate toward the left or right side of the frontal visual object and retain this conditioned visual orientation. The lower and upper visual fields have different roles in conditioned visual orientation. Transfer experiments showed that conditioned visual orientation could generalize between visual targets of different sizes, compactness, or vertical positions, but not of contour orientation. Rut—Type I adenylyl cyclase and Dnc—phosphodiesterase were dispensable for visual orientation conditioning. Normal activity and scb signaling in R3/R4d neurons of the ellipsoid body were required for visual orientation conditioning. Our studies established a visual orientation conditioning paradigm and examined the behavioral properties and neural circuitry of visual orientation, an important component of the insect's spatial navigation. PMID:25512578
The free-flight response of Drosophila to motion of the visual environment.
Mronz, Markus; Lehmann, Fritz-Olaf
2008-07-01
In the present study we investigated the behavioural strategies with which freely flying fruit flies (Drosophila) control their flight trajectories during active optomotor stimulation in a free-flight arena. We measured forward, turning and climbing velocities of single flies using high-speed video analysis and estimated the output of a 'Hassenstein-Reichardt' elementary motion detector (EMD) array and the fly's gaze to evaluate flight behaviour in response to a rotating visual panorama. In a stationary visual environment, flight is characterized by flight saccades during which the animals turn on average 120 degrees within 130 ms. In a rotating environment, the fly's behaviour typically changes towards distinct, concentric circular flight paths where the radius of the paths increases with increasing arena velocity. The EMD simulation suggests that this behaviour is driven by a rotation-sensitive EMD detector system that minimizes retinal slip on each compound eye, whereas an expansion-sensitive EMD system with a laterally centred visual focus potentially helps to achieve centring response on the circular flight path. We developed a numerical model based on force balance between horizontal, vertical and lateral forces that allows predictions of flight path curvature at a given locomotor capacity of the fly. The model suggests that turning flight in Drosophila is constrained by the production of centripetal forces needed to avoid side-slip movements. At maximum horizontal velocity this force may account for up to 70% of the fly's body weight during yaw turning. Altogether, our analyses are widely consistent with previous studies on Drosophila free flight and those on the optomotor response under tethered flight conditions.
Efficient encoding of motion is mediated by gap junctions in the fly visual system.
Wang, Siwei; Borst, Alexander; Zaslavsky, Noga; Tishby, Naftali; Segev, Idan
2017-12-01
Understanding the computational implications of specific synaptic connectivity patterns is a fundamental goal in neuroscience. In particular, the computational role of ubiquitous electrical synapses operating via gap junctions remains elusive. In the fly visual system, the cells in the vertical-system network, which play a key role in visual processing, primarily connect to each other via axonal gap junctions. This network therefore provides a unique opportunity to explore the functional role of gap junctions in sensory information processing. Our information theoretical analysis of a realistic VS network model shows that within 10 ms following the onset of the visual input, the presence of axonal gap junctions enables the VS system to efficiently encode the axis of rotation, θ, of the fly's ego motion. This encoding efficiency, measured in bits, is near-optimal with respect to the physical limits of performance determined by the statistical structure of the visual input itself. The VS network is known to be connected to downstream pathways via a subset of triplets of the vertical system cells; we found that because of the axonal gap junctions, the efficiency of this subpopulation in encoding θ is superior to that of the whole vertical system network and is robust to a wide range of signal to noise ratios. We further demonstrate that this efficient encoding of motion by this subpopulation is necessary for the fly's visually guided behavior, such as banked turns in evasive maneuvers. Because gap junctions are formed among the axons of the vertical system cells, they only impact the system's readout, while maintaining the dendritic input intact, suggesting that the computational principles implemented by neural circuitries may be much richer than previously appreciated based on point neuron models. Our study provides new insights as to how specific network connectivity leads to efficient encoding of sensory stimuli.
A magnetic tether system to investigate visual and olfactory mediated flight control in Drosophila.
Duistermars, Brian J; Frye, Mark
2008-11-21
It has been clear for many years that insects use visual cues to stabilize their heading in a wind stream. Many animals track odors carried in the wind. As such, visual stabilization of upwind tracking directly aids in odor tracking. But do olfactory signals directly influence visual tracking behavior independently from wind cues? Also, the recent deluge of research on the neurophysiology and neurobehavioral genetics of olfaction in Drosophila has motivated ever more technically sophisticated and quantitative behavioral assays. Here, we modified a magnetic tether system originally devised for vision experiments by equipping the arena with narrow laminar flow odor plumes. A fly is glued to a small steel pin and suspended in a magnetic field that enables it to yaw freely. Small diameter food odor plumes are directed downward over the fly's head, eliciting stable tracking by a hungry fly. Here we focus on the critical mechanics of tethering, aligning the magnets, devising the odor plume, and confirming stable odor tracking.
Optic flow-based collision-free strategies: From insects to robots.
Serres, Julien R; Ruffier, Franck
2017-09-01
Flying insects are able to fly smartly in an unpredictable environment. It has been found that flying insects have smart neurons inside their tiny brains that are sensitive to visual motion also called optic flow. Consequently, flying insects rely mainly on visual motion during their flight maneuvers such as: takeoff or landing, terrain following, tunnel crossing, lateral and frontal obstacle avoidance, and adjusting flight speed in a cluttered environment. Optic flow can be defined as the vector field of the apparent motion of objects, surfaces, and edges in a visual scene generated by the relative motion between an observer (an eye or a camera) and the scene. Translational optic flow is particularly interesting for short-range navigation because it depends on the ratio between (i) the relative linear speed of the visual scene with respect to the observer and (ii) the distance of the observer from obstacles in the surrounding environment without any direct measurement of either speed or distance. In flying insects, roll stabilization reflex and yaw saccades attenuate any rotation at the eye level in roll and yaw respectively (i.e. to cancel any rotational optic flow) in order to ensure pure translational optic flow between two successive saccades. Our survey focuses on feedback-loops which use the translational optic flow that insects employ for collision-free navigation. Optic flow is likely, over the next decade to be one of the most important visual cues that can explain flying insects' behaviors for short-range navigation maneuvers in complex tunnels. Conversely, the biorobotic approach can therefore help to develop innovative flight control systems for flying robots with the aim of mimicking flying insects' abilities and better understanding their flight. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.
Schlieren photography on freely flying hawkmoth.
Liu, Yun; Roll, Jesse; Van Kooten, Stephen; Deng, Xinyan
2018-05-01
The aerodynamic force on flying insects results from the vortical flow structures that vary both spatially and temporally throughout flight. Due to these complexities and the inherent difficulties in studying flying insects in a natural setting, a complete picture of the vortical flow has been difficult to obtain experimentally. In this paper, Schlieren , a widely used technique for highspeed flow visualization, was adapted to capture the vortex structures around freely flying hawkmoth ( Manduca ). Flow features such as leading-edge vortex, trailing-edge vortex, as well as the full vortex system in the wake were visualized directly. Quantification of the flow from the Schlieren images was then obtained by applying a physics-based optical flow method, extending the potential applications of the method to further studies of flying insects. © 2018 The Author(s).
Funk, Agnes P; Rosa, Marcello G P
1998-01-01
The first (V1) and second (V2) cortical visual areas exist in all mammals. However, the functional relationship between these areas varies between species. While in monkeys the responses of V2 cells depend on inputs from V1, in all non-primates studied so far V2 cells largely retain responsiveness to photic stimuli after destruction of V1.We studied the visual responsiveness of neurones in V2 of flying foxes after total or partial lesions of the primary visual cortex (V1). The main finding was that visual responses can be evoked in the region of V2 corresponding, in visuotopic co-ordinates, to the lesioned portion of V1 (‘lesion projection zone’; LPZ).The visuotopic organization of V2 was not altered by V1 lesions.The proportion of neurones with strong visual responses was significantly lower within the LPZs (31.5 %) than outside these zones, or in non-lesioned control hemispheres (> 70 %). LPZ cells showed weak direction and orientation bias, and responded consistently only at low spatial and temporal frequencies.The data demonstrate that the functional relationship between V1 and V2 of flying foxes resembles that observed in non-primate mammals. This observation contrasts with the ‘primate-like’ characteristics of the flying fox visual system reported by previous studies. PMID:9806999
Micro-computed tomography visualization of the vestigial alimentary canal in adult oestrid flies.
Martín-Vega, D; Garbout, A; Ahmed, F; Ferrer, L M; Lucientes, J; Colwell, D D; Hall, M J R
2018-02-16
Oestrid flies (Diptera: Oestridae) do not feed during the adult stage as they acquire all necessary nutrients during the parasitic larval stage. The adult mouthparts and digestive tract are therefore frequently vestigial; however, morphological data on the alimentary canal in adult oestrid flies are scarce and a proper visualization of this organ system within the adult body is lacking. The present work visualizes the morphology of the alimentary canal in adults of two oestrid species, Oestrus ovis L. and Hypoderma lineatum (de Villiers), with the use of non-invasive micro-computed tomography (micro-CT) and compares it with the highly developed alimentary canal of the blow fly Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae). Both O. ovis and H. lineatum adults showed significant reductions of the cardia and the diameter of the digestive tract, an absence of the helicoidal portion of the midgut typical of other cyclorrhaphous flies, and a lack of crop and salivary glands. Given the current interest in the alimentary canal in adult dipterans in biomedical and developmental biology studies, further understanding of the morphology and development of this organ system in adult oestrids may provide valuable new insights in several areas of research. © 2018 The Royal Entomological Society.
A Conserved Developmental Mechanism Builds Complex Visual Systems in Insects and Vertebrates
Joly, Jean-Stéphane; Recher, Gaelle; Brombin, Alessandro; Ngo, Kathy; Hartenstein, Volker
2016-01-01
The visual systems of vertebrates and many other bilaterian clades consist of complex neural structures guiding a wide spectrum of behaviors. Homologies at the level of cell types and even discrete neural circuits have been proposed, but many questions of how the architecture of visual neuropils evolved among different phyla remain open. In this review we argue that the profound conservation of genetic and developmental steps generating the eye and its target neuropils in fish and fruit flies supports a homology between some core elements of bilaterian visual circuitries. Fish retina and tectum, and fly optic lobe, develop from a partitioned, unidirectionally proliferating neurectodermal domain that combines slowly dividing neuroepithelial stem cells and rapidly amplifying progenitors with shared genetic signatures to generate large numbers and different types of neurons in a temporally ordered way. This peculiar ‘conveyor belt neurogenesis’ could play an essential role in generating the topographically ordered circuitry of the visual system. PMID:27780043
Visual Control for Multirobot Organized Rendezvous.
Lopez-Nicolas, G; Aranda, M; Mezouar, Y; Sagues, C
2012-08-01
This paper addresses the problem of visual control of a set of mobile robots. In our framework, the perception system consists of an uncalibrated flying camera performing an unknown general motion. The robots are assumed to undergo planar motion considering nonholonomic constraints. The goal of the control task is to drive the multirobot system to a desired rendezvous configuration relying solely on visual information given by the flying camera. The desired multirobot configuration is defined with an image of the set of robots in that configuration without any additional information. We propose a homography-based framework relying on the homography induced by the multirobot system that gives a desired homography to be used to define the reference target, and a new image-based control law that drives the robots to the desired configuration by imposing a rigidity constraint. This paper extends our previous work, and the main contributions are that the motion constraints on the flying camera are removed, the control law is improved by reducing the number of required steps, the stability of the new control law is proved, and real experiments are provided to validate the proposal.
Modeling of the First Layers in the Fly's Eye
NASA Technical Reports Server (NTRS)
Moya, J. A.; Wilcox, M. J.; Donohoe, G. W.
1997-01-01
Increased autonomy of robots would yield significant advantages in the exploration of space. The shortfalls of computer vision can, however, pose significant limitations on a robot's potential. At the same time, simple insects which are largely hard-wired have effective visual systems. The understanding of insect vision systems thus may lead to improved approaches to visual tasks. A good starting point for the study of a vision system is its eye. In this paper, a model of the sensory portion of the fly's eye is presented. The effectiveness of the model is briefly addressed by a comparison of its performance to experimental data.
Cooperative Monocular-Based SLAM for Multi-UAV Systems in GPS-Denied Environments †
Guerra, Edmundo
2018-01-01
This work presents a cooperative monocular-based SLAM approach for multi-UAV systems that can operate in GPS-denied environments. The main contribution of the work is to show that, using visual information obtained from monocular cameras mounted onboard aerial vehicles flying in formation, the observability properties of the whole system are improved. This fact is especially notorious when compared with other related visual SLAM configurations. In order to improve the observability properties, some measurements of the relative distance between the UAVs are included in the system. These relative distances are also obtained from visual information. The proposed approach is theoretically validated by means of a nonlinear observability analysis. Furthermore, an extensive set of computer simulations is presented in order to validate the proposed approach. The numerical simulation results show that the proposed system is able to provide a good position and orientation estimation of the aerial vehicles flying in formation. PMID:29701722
Cooperative Monocular-Based SLAM for Multi-UAV Systems in GPS-Denied Environments.
Trujillo, Juan-Carlos; Munguia, Rodrigo; Guerra, Edmundo; Grau, Antoni
2018-04-26
This work presents a cooperative monocular-based SLAM approach for multi-UAV systems that can operate in GPS-denied environments. The main contribution of the work is to show that, using visual information obtained from monocular cameras mounted onboard aerial vehicles flying in formation, the observability properties of the whole system are improved. This fact is especially notorious when compared with other related visual SLAM configurations. In order to improve the observability properties, some measurements of the relative distance between the UAVs are included in the system. These relative distances are also obtained from visual information. The proposed approach is theoretically validated by means of a nonlinear observability analysis. Furthermore, an extensive set of computer simulations is presented in order to validate the proposed approach. The numerical simulation results show that the proposed system is able to provide a good position and orientation estimation of the aerial vehicles flying in formation.
Code of Federal Regulations, 2010 CFR
2010-01-01
... pilots flying under visual flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME... Rules Visual Flight Rules § 91.161 Special awareness training required for pilots flying under visual...-nautical mile radius of the Washington, DC VOR/DME under visual flight rules (VFR). Except as provided...
Adaptation of velocity encoding in synaptically coupled neurons in the fly visual system.
Kalb, Julia; Egelhaaf, Martin; Kurtz, Rafael
2008-09-10
Although many adaptation-induced effects on neuronal response properties have been described, it is often unknown at what processing stages in the nervous system they are generated. We focused on fly visual motion-sensitive neurons to identify changes in response characteristics during prolonged visual motion stimulation. By simultaneous recordings of synaptically coupled neurons, we were able to directly compare adaptation-induced effects at two consecutive processing stages in the fly visual motion pathway. This allowed us to narrow the potential sites of adaptation effects within the visual system and to relate them to the properties of signal transfer between neurons. Motion adaptation was accompanied by a response reduction, which was somewhat stronger in postsynaptic than in presynaptic cells. We found that the linear representation of motion velocity degrades during adaptation to a white-noise velocity-modulated stimulus. This effect is caused by an increasingly nonlinear velocity representation rather than by an increase of noise and is similarly strong in presynaptic and postsynaptic neurons. In accordance with this similarity, the dynamics and the reliability of interneuronal signal transfer remained nearly constant. Thus, adaptation is mainly based on processes located in the presynaptic neuron or in more peripheral processing stages. In contrast, changes of transfer properties at the analyzed synapse or in postsynaptic spike generation contribute little to changes in velocity coding during motion adaptation.
Visual learning in drosophila: application on a roving robot and comparisons
NASA Astrophysics Data System (ADS)
Arena, P.; De Fiore, S.; Patané, L.; Termini, P. S.; Strauss, R.
2011-05-01
Visual learning is an important aspect of fly life. Flies are able to extract visual cues from objects, like colors, vertical and horizontal distributedness, and others, that can be used for learning to associate a meaning to specific features (i.e. a reward or a punishment). Interesting biological experiments show trained stationary flying flies avoiding flying towards specific visual objects, appearing on the surrounding environment. Wild-type flies effectively learn to avoid those objects but this is not the case for the learning mutant rutabaga defective in the cyclic AMP dependent pathway for plasticity. A bio-inspired architecture has been proposed to model the fly behavior and experiments on roving robots were performed. Statistical comparisons have been considered and mutant-like effect on the model has been also investigated.
Behavioral consequences of dopamine deficiency in the Drosophila central nervous system
Riemensperger, Thomas; Isabel, Guillaume; Coulom, Hélène; Neuser, Kirsa; Seugnet, Laurent; Kume, Kazuhiko; Iché-Torres, Magali; Cassar, Marlène; Strauss, Roland; Preat, Thomas; Hirsh, Jay; Birman, Serge
2011-01-01
The neuromodulatory function of dopamine (DA) is an inherent feature of nervous systems of all animals. To learn more about the function of neural DA in Drosophila, we generated mutant flies that lack tyrosine hydroxylase, and thus DA biosynthesis, selectively in the nervous system. We found that DA is absent or below detection limits in the adult brain of these flies. Despite this, they have a lifespan similar to WT flies. These mutants show reduced activity, extended sleep time, locomotor deficits that increase with age, and they are hypophagic. Whereas odor and electrical shock avoidance are not affected, aversive olfactory learning is abolished. Instead, DA-deficient flies have an apparently “masochistic” tendency to prefer the shock-associated odor 2 h after conditioning. Similarly, sugar preference is absent, whereas sugar stimulation of foreleg taste neurons induces normal proboscis extension. Feeding the DA precursor l-DOPA to adults substantially rescues the learning deficit as well as other impaired behaviors that were tested. DA-deficient flies are also defective in positive phototaxis, without alteration in visual perception and optomotor response. Surprisingly, visual tracking is largely maintained, and these mutants still possess an efficient spatial orientation memory. Our findings show that flies can perform complex brain functions in the absence of neural DA, whereas specific behaviors involving, in particular, arousal and choice require normal levels of this neuromodulator. PMID:21187381
Mutational Analysis of Drosophila Basigin Function in the Visual System
Munro, Michelle; Akkam, Yazan; Curtin, Kathryn D.
2009-01-01
Drosophila basigin is a cell-surface glycoprotein of the Ig superfamily and a member of a protein family that includes mammalian EMMPRIN/CD147/basigin, neuroplastin, and embigin. Our previous work on Drosophila basigin has shown that it is required for normal photoreceptor cell structure and normal neuron-glia interaction in the fly visual system. Specifically, the photoreceptor neurons of mosaic animals that are mutant in the eye for basigin show altered cell structure with nuclei, mitochondria and rER misplaced and variable axon diameter compared to wild-type. In addition, glia cells in the optic lamina that contact photoreceptor axons are misplaced and show altered structure. All these defects are rescued by expression of either transgenic fly basigin or transgenic mouse basigin in the photoreceptors demonstrating that mouse basigin can functionally replace fly basigin. To determine what regions of the basigin protein are required for each of these functions, we have created mutant basigin transgenes coding for proteins that are altered in conserved residues, introduced these into the fly genome, and tested them for their ability to rescue both photoreceptor cell structure defects and neuron-glia interaction defects of basigin. The results suggest that the highly conserved transmembrane domain and the extracellular domains are crucial for basigin function in the visual system while the short intracellular tail may not play a role in these functions. PMID:19782733
Barron, Andrew; Srinivasan, Mandyam V
2006-03-01
There is now increasing evidence that honey bees regulate their ground speed in flight by holding constant the speed at which the image of the environment moves across the eye (optic flow). We have investigated the extent to which ground speed is affected by headwinds. Honey bees were trained to enter a tunnel to forage at a sucrose feeder placed at its far end. Ground speeds in the tunnel were recorded while systematically varying the visual texture of the tunnel, and the strength of headwinds experienced by the flying bees. We found that in a flight tunnel bees used visual cues to maintain their ground speed, and adjusted their air speed to maintain a constant rate of optic flow, even against headwinds which were, at their strongest, 50% of a bee's maximum recorded forward velocity. Manipulation of the visual texture revealed that headwind is compensated almost fully even when the optic flow cues are very sparse and subtle, demonstrating the robustness of this visual flight control system. We discuss these findings in the context of field observations of flying bees.
Peripheral Processing Facilitates Optic Flow-Based Depth Perception
Li, Jinglin; Lindemann, Jens P.; Egelhaaf, Martin
2016-01-01
Flying insects, such as flies or bees, rely on consistent information regarding the depth structure of the environment when performing their flight maneuvers in cluttered natural environments. These behaviors include avoiding collisions, approaching targets or spatial navigation. Insects are thought to obtain depth information visually from the retinal image displacements (“optic flow”) during translational ego-motion. Optic flow in the insect visual system is processed by a mechanism that can be modeled by correlation-type elementary motion detectors (EMDs). However, it is still an open question how spatial information can be extracted reliably from the responses of the highly contrast- and pattern-dependent EMD responses, especially if the vast range of light intensities encountered in natural environments is taken into account. This question will be addressed here by systematically modeling the peripheral visual system of flies, including various adaptive mechanisms. Different model variants of the peripheral visual system were stimulated with image sequences that mimic the panoramic visual input during translational ego-motion in various natural environments, and the resulting peripheral signals were fed into an array of EMDs. We characterized the influence of each peripheral computational unit on the representation of spatial information in the EMD responses. Our model simulations reveal that information about the overall light level needs to be eliminated from the EMD input as is accomplished under light-adapted conditions in the insect peripheral visual system. The response characteristics of large monopolar cells (LMCs) resemble that of a band-pass filter, which reduces the contrast dependency of EMDs strongly, effectively enhancing the representation of the nearness of objects and, especially, of their contours. We furthermore show that local brightness adaptation of photoreceptors allows for spatial vision under a wide range of dynamic light conditions. PMID:27818631
Flight performance in night-flying sweat bees suffers at low light levels.
Theobald, Jamie Carroll; Coates, Melissa M; Wcislo, William T; Warrant, Eric J
2007-11-01
The sweat bee Megalopta (Hymenoptera: Halictidae), unlike most bees, flies in extremely dim light. And although nocturnal insects are often equipped with superposition eyes, which greatly enhance light capture, Megalopta performs visually guided flight with apposition eyes. We examined how light limits Megalopta's flight behavior by measuring flight times and corresponding light levels and comparing them with flight trajectories upon return to the nest. We found the average time to land increased in dim light, an effect due not to slow approaches, but to circuitous approaches. Some landings, however, were quite fast even in the dark. To explain this, we examined the flight trajectories and found that in dim light, landings became increasingly error prone and erratic, consistent with repeated landing attempts. These data agree well with the premise that Megalopta uses visual summation, sacrificing acuity in order to see and fly at the very dimmest light intensities that its visual system allows.
Context-dependent olfactory enhancement of optomotor flight control in Drosophila.
Chow, Dawnis M; Frye, Mark A
2008-08-01
Sensing and following the chemical plume of food odors is a fundamental challenge faced by many organisms. For flying insects, the task is complicated by wind that distorts the plume and buffets the fly. To maintain an upwind heading, and thus stabilize their orientation in a plume, insects such as flies and moths make use of strong context-specific visual equilibrium reflexes. For example, flying straight requires the regulation of image rotation across the eye, whereas minimizing side-slip and avoiding a collision require regulation of image expansion. In flies, visual rotation stabilizes plume tracking, but rotation and expansion optomotor responses are controlled by separate visual pathways. Are olfactory signals integrated with optomotor responses in a manner dependent upon visual context? We addressed this question by investigating the effect of an attractive food odor on active optomotor flight control. Odorant caused flies both to increase aerodynamic power output and to steer straighter. However, when challenged with wide-field optic flow, odor resulted in enhanced amplitude rotation responses but reduced amplitude expansion responses. For both visual conditions, flies tracked motion signals more closely in odor, an indication of increased saliency. These results suggest a simple search algorithm by which olfactory signals improve the salience of visual stimuli and modify optomotor control in a context-dependent manner, thereby enabling an animal to fly straight up a plume and approach odiferous objects.
Interaction of feel system and flight control system dynamics on lateral flying qualities
NASA Technical Reports Server (NTRS)
Bailey, R. E.; Knotts, L. H.
1990-01-01
An experimental investigation of the influence of lateral feel system characteristics on fighter aircraft roll flying qualities was conducted using the variable stability USAF NT-33. Forty-two evaluation flights were flown by three engineering test pilots. The investigation utilized the power approach, visual landing task and up-and-away tasks including formation, gun tracking, and computer-generated compensatory attitude tracking tasks displayed on the Head-Up Display. Experimental variations included the feel system frequency, force-deflection gradient, control system command type (force or position input command), aircraft roll mode time constant, control system prefilter frequency, and control system time delay. The primary data were task performance records and evaluation pilot comments and ratings using the Cooper-Harper scale. The data highlight the unique and powerful effect of the feel system of flying qualities. The data show that the feel system is not 'equivalent' in flying qualities influence to analogous control system elements. A lower limit of allowable feel system frequency appears warranted to ensure good lateral flying qualities. Flying qualities criteria should most properly treat the feel system dynamic influence separately from the control system, since the input and output of this dynamic element is apparent to the pilot and thus, does not produce a 'hidden' effect.
Robie, Alice A.; Straw, Andrew D.; Dickinson, Michael H.
2010-01-01
Walking fruit flies, Drosophila melanogaster, use visual information to orient towards salient objects in their environment, presumably as a search strategy for finding food, shelter or other resources. Less is known, however, about the role of vision or other sensory modalities such as mechanoreception in the evaluation of objects once they have been reached. To study the role of vision and mechanoreception in exploration behavior, we developed a large arena in which we could track individual fruit flies as they walked through either simple or more topologically complex landscapes. When exploring a simple, flat environment lacking three-dimensional objects, flies used visual cues from the distant background to stabilize their walking trajectories. When exploring an arena containing an array of cones, differing in geometry, flies actively oriented towards, climbed onto, and explored the objects, spending most of their time on the tallest, steepest object. A fly's behavioral response to the geometry of an object depended upon the intrinsic properties of each object and not a relative assessment to other nearby objects. Furthermore, the preference was not due to a greater attraction towards tall, steep objects, but rather a change in locomotor behavior once a fly reached and explored the surface. Specifically, flies are much more likely to stop walking for long periods when they are perched on tall, steep objects. Both the vision system and the antennal chordotonal organs (Johnston's organs) provide sufficient information about the geometry of an object to elicit the observed change in locomotor behavior. Only when both these sensory systems were impaired did flies not show the behavioral preference for the tall, steep objects. PMID:20581279
Turning behaviour depends on frictional damping in the fruit fly Drosophila.
Hesselberg, Thomas; Lehmann, Fritz-Olaf
2007-12-01
Turning behaviour in the fruit fly Drosophila depends on several factors including not only feedback from sensory organs and muscular control of wing motion, but also the mass moments of inertia and the frictional damping coefficient of the rotating body. In the present study we evaluate the significance of body friction for yaw turning and thus the limits of visually mediated flight control in Drosophila, by scoring tethered flies flying in a flight simulator on their ability to visually compensate a bias on a moving object and a visual background panorama at different simulated frictional dampings. We estimated the fly's natural damping coefficient from a numerical aerodynamic model based on both friction on the body and the flapping wings during saccadic turning. The model predicts a coefficient of 54 x 10(-12) Nm s, which is more than 100-times larger than the value estimated from a previous study on the body alone. Our estimate suggests that friction plays a larger role for yaw turning in Drosophila than moments of inertia. The simulator experiments showed that visual performance of the fruit fly collapses near the physical conditions estimated for freely flying animals, which is consistent with the suggested role of the halteres for flight stabilization. However, kinematic analyses indicate that the measured loss of flight control might be due predominantly to the limited fine control in the fly's steering muscles below a threshold of 1-2 degrees stroke amplitude, rather than resulting from the limits of visual motion detection by the fly's compound eyes. We discuss the impact of these results and suggest that the elevated frictional coefficient permits freely flying fruit flies to passively terminate rotational body movements without producing counter-torque during the second half of the saccadic turning manoeuvre.
3D Visualization of Cooperative Trajectories
NASA Technical Reports Server (NTRS)
Schaefer, John A.
2014-01-01
Aerodynamicists and biologists have long recognized the benefits of formation flight. When birds or aircraft fly in the upwash region of the vortex generated by leaders in a formation, induced drag is reduced for the trail bird or aircraft, and efficiency improves. The major consequence of this is that fuel consumption can be greatly reduced. When two aircraft are separated by a large enough longitudinal distance, the aircraft are said to be flying in a cooperative trajectory. A simulation has been developed to model autonomous cooperative trajectories of aircraft; however it does not provide any 3D representation of the multi-body system dynamics. The topic of this research is the development of an accurate visualization of the multi-body system observable in a 3D environment. This visualization includes two aircraft (lead and trail), a landscape for a static reference, and simplified models of the vortex dynamics and trajectories at several locations between the aircraft.
Cues used by the black fly, Simulium annulus, for attraction to the common loon (Gavia immer).
Weinandt, Meggin L; Meyer, Michael; Strand, Mac; Lindsay, Alec R
2012-12-01
The parasitic relationship between a black fly, Simulium annulus, and the common loon (Gavia immer) has been considered one of the most exclusive relationships between any host species and a black fly species. To test the host specificity of this blood-feeding insect, we made a series of bird decoy presentations to black flies on loon-inhabited lakes in northern Wisconsin, U.S.A. To examine the importance of chemical and visual cues for black fly detection of and attraction to hosts, we made decoy presentations with and without chemical cues. Flies attracted to the decoys were collected, identified to species, and quantified. Results showed that S. annulus had a strong preference for common loon visual and chemical cues, although visual cues from Canada geese (Branta canadensis) and mallards (Anas platyrynchos) did attract some flies in significantly smaller numbers. © 2012 The Society for Vector Ecology.
Colomb, Julien; Reiter, Lutz; Blaszkiewicz, Jedrzej; Wessnitzer, Jan; Brembs, Bjoern
2012-01-01
Insects have been among the most widely used model systems for studying the control of locomotion by nervous systems. In Drosophila, we implemented a simple test for locomotion: in Buridan's paradigm, flies walk back and forth between two inaccessible visual targets [1]. Until today, the lack of easily accessible tools for tracking the fly position and analyzing its trajectory has probably contributed to the slow acceptance of Buridan's paradigm. We present here a package of open source software designed to track a single animal walking in a homogenous environment (Buritrack) and to analyze its trajectory. The Centroid Trajectory Analysis (CeTrAn) software is coded in the open source statistics project R. It extracts eleven metrics and includes correlation analyses and a Principal Components Analysis (PCA). It was designed to be easily customized to personal requirements. In combination with inexpensive hardware, these tools can readily be used for teaching and research purposes. We demonstrate the capabilities of our package by measuring the locomotor behavior of adult Drosophila melanogaster (whose wings were clipped), either in the presence or in the absence of visual targets, and comparing the latter to different computer-generated data. The analysis of the trajectories confirms that flies are centrophobic and shows that inaccessible visual targets can alter the orientation of the flies without changing their overall patterns of activity. Using computer generated data, the analysis software was tested, and chance values for some metrics (as well as chance value for their correlation) were set. Our results prompt the hypothesis that fixation behavior is observed only if negative phototaxis can overcome the propensity of the flies to avoid the center of the platform. Together with our companion paper, we provide new tools to promote Open Science as well as the collection and analysis of digital behavioral data.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Bae, Sungchul; Meral, Cagla; Department of Civil Engineering, Middle East Technical University, 06800 Ankara
2014-05-01
The present study focuses on identification and micro-structural characterization of the hydration products formed in high-volume fly ash (HVFA)/portland cement (PC) systems using monochromatic scanning x-ray micro-diffraction (μ-SXRD) and SEM-EDS. Pastes with up to 80% fly ash replacement were studied. Phase maps for HVFA samples using μ-SXRD patterns prove that μ-SXRD is an effective method to identify and visualize the distribution of phases in the matrix. μ-SXRD and SEM-EDS analysis shows that the C-S-H formed in HVFA system containing 50% or more of fly ash has a similar structure as C-S-H(I) with comparatively lower Ca/Si ratio than the one producedmore » in PC system. Moreover, coexistence of C-S-H(I) and strätlingite is observed in the system containing 80% of fly ash, confirming that the amount of alumina and silicate phases provided by the fly ash is a major factor for the formation of C-S-H(I) and strätlingite in HVFA system. - Highlights: • High-volume fly ash (HVFA) paste was studied by scanning x-ray micro-diffraction. • Coexistence of C-S-H(I) and strätlingite in the HVFA system is clearly shown. • The distribution of minor phases in the HVFA system is shown. • Differences between inner and outer products of fly ash are observed by SEM-EDS.« less
Huang, Xiaojing; Lauer, Kenneth; Clark, Jesse N.; ...
2015-03-13
We report an experimental ptychography measurement performed in fly-scan mode. With a visible-light laser source, we demonstrate a 5-fold reduction of data acquisition time. By including multiple mutually incoherent modes into the incident illumination, high quality images were successfully reconstructed from blurry diffraction patterns. Thus, this approach significantly increases the throughput of ptychography, especially for three-dimensional applications and the visualization of dynamic systems.
Experience improves feature extraction in Drosophila.
Peng, Yueqing; Xi, Wang; Zhang, Wei; Zhang, Ke; Guo, Aike
2007-05-09
Previous exposure to a pattern in the visual scene can enhance subsequent recognition of that pattern in many species from honeybees to humans. However, whether previous experience with a visual feature of an object, such as color or shape, can also facilitate later recognition of that particular feature from multiple visual features is largely unknown. Visual feature extraction is the ability to select the key component from multiple visual features. Using a visual flight simulator, we designed a novel protocol for visual feature extraction to investigate the effects of previous experience on visual reinforcement learning in Drosophila. We found that, after conditioning with a visual feature of objects among combinatorial shape-color features, wild-type flies exhibited poor ability to extract the correct visual feature. However, the ability for visual feature extraction was greatly enhanced in flies trained previously with that visual feature alone. Moreover, we demonstrated that flies might possess the ability to extract the abstract category of "shape" but not a particular shape. Finally, this experience-dependent feature extraction is absent in flies with defective MBs, one of the central brain structures in Drosophila. Our results indicate that previous experience can enhance visual feature extraction in Drosophila and that MBs are required for this experience-dependent visual cognition.
Architecture of a platform for hardware-in-the-loop simulation of flying vehicle control systems
NASA Astrophysics Data System (ADS)
Belokon', S. A.; Zolotukhin, Yu. N.; Filippov, M. N.
2017-07-01
A hardware-software platform is presented, which is designed for the development and hardware-in-the-loop simulation of flying vehicle control systems. This platform ensures the construction of the mathematical model of the plant, development of algorithms and software for onboard radioelectronic equipment and ground control station, and visualization of the three-dimensional model of the vehicle and external environment of the cockpit in the simulator training mode.
Enhanced trapping of stable flies via olfactory and visual cues
USDA-ARS?s Scientific Manuscript database
Adult stable flies are highly attracted to the so-called Alsynite cylinder trap; however this trap is expensive. Here we report the development of a cheaper and better white panel trap with options of adding visual and olfactory stimuli for enhanced stable fly trapping. The white panel trap attracte...
Primary display latency criteria based on flying qualities and performance data
NASA Technical Reports Server (NTRS)
Funk, John D., Jr.; Beck, Corin P.; Johns, John B.
1993-01-01
With a pilots' increasing use of visual cue augmentation, much requiring extensive pre-processing, there is a need to establish criteria for new avionics/display design. The timeliness and synchronization of the augmented cues is vital to ensure the performance quality required for precision mission task elements (MTEs) where augmented cues are the primary source of information to the pilot. Processing delays incurred while transforming sensor-supplied flight information into visual cues are unavoidable. Relationships between maximum control system delays and associated flying qualities levels are documented in MIL-F-83300 and MIL-F-8785. While cues representing aircraft status may be just as vital to the pilot as prompt control response for operations in instrument meteorological conditions, presently, there are no specification requirements on avionics system latency. To produce data relating avionics system latency to degradations in flying qualities, the Navy conducted two simulation investigations. During the investigations, flying qualities and performance data were recorded as simulated avionics system latency was varied. Correlated results of the investigation indicates that there is a detrimental impact of latency on flying qualities. Analysis of these results and consideration of key factors influencing their application indicate that: (1) Task performance degrades and pilot workload increases as latency is increased. Inconsistency in task performance increases as latency increases. (2) Latency reduces the probability of achieving Level 1 handling qualities with avionics system latency as low as 70 ms. (3) The data suggest that the achievement of desired performance will be ensured only at display latency values below 120 ms. (4) These data also suggest that avoidance of inadequate performance will be ensured only at display latency values below 150 ms.
Dissociation of visual associative and motor learning in Drosophila at the flight simulator.
Wang, Shunpeng; Li, Yan; Feng, Chunhua; Guo, Aike
2003-08-29
Ever since operant conditioning was studied experimentally, the relationship between associative learning and possible motor learning has become controversial. Although motor learning and its underlying neural substrates have been extensively studied in mammals, it is still poorly understood in invertebrates. The visual discriminative avoidance paradigm of Drosophila at the flight simulator has been widely used to study the flies' visual associative learning and related functions, but it has not been used to study the motor learning process. In this study, newly-designed data analysis was employed to examine the flies' solitary behavioural variable that was recorded at the flight simulator-yaw torque. Analysis was conducted to explore torque distributions of both wild-type and mutant flies in conditioning, with the following results: (1) Wild-type Canton-S flies had motor learning performance in conditioning, which was proved by modifications of the animal's behavioural mode in conditioning. (2) Repetition of training improved the motor learning performance of wild-type Canton-S flies. (3) Although mutant dunce(1) flies were defective in visual associative learning, they showed essentially normal motor learning performance in terms of yaw torque distribution in conditioning. Finally, we tentatively proposed that both visual associative learning and motor learning were involved in the visual operant conditioning of Drosophila at the flight simulator, that the two learning forms could be dissociated and they might have different neural bases.
The role of visual and mechanosensory cues in structuring forward flight in Drosophila melanogaster.
Budick, Seth A; Reiser, Michael B; Dickinson, Michael H
2007-12-01
It has long been known that many flying insects use visual cues to orient with respect to the wind and to control their groundspeed in the face of varying wind conditions. Much less explored has been the role of mechanosensory cues in orienting insects relative to the ambient air. Here we show that Drosophila melanogaster, magnetically tethered so as to be able to rotate about their yaw axis, are able to detect and orient into a wind, as would be experienced during forward flight. Further, this behavior is velocity dependent and is likely subserved, at least in part, by the Johnston's organs, chordotonal organs in the antennae also involved in near-field sound detection. These wind-mediated responses may help to explain how flies are able to fly forward despite visual responses that might otherwise inhibit this behavior. Expanding visual stimuli, such as are encountered during forward flight, are the most potent aversive visual cues known for D. melanogaster flying in a tethered paradigm. Accordingly, tethered flies strongly orient towards a focus of contraction, a problematic situation for any animal attempting to fly forward. We show in this study that wind stimuli, transduced via mechanosensory means, can compensate for the aversion to visual expansion and thus may help to explain how these animals are indeed able to maintain forward flight.
FlyCap: Markerless Motion Capture Using Multiple Autonomous Flying Cameras.
Xu, Lan; Liu, Yebin; Cheng, Wei; Guo, Kaiwen; Zhou, Guyue; Dai, Qionghai; Fang, Lu
2017-07-18
Aiming at automatic, convenient and non-instrusive motion capture, this paper presents a new generation markerless motion capture technique, the FlyCap system, to capture surface motions of moving characters using multiple autonomous flying cameras (autonomous unmanned aerial vehicles(UAVs) each integrated with an RGBD video camera). During data capture, three cooperative flying cameras automatically track and follow the moving target who performs large-scale motions in a wide space. We propose a novel non-rigid surface registration method to track and fuse the depth of the three flying cameras for surface motion tracking of the moving target, and simultaneously calculate the pose of each flying camera. We leverage the using of visual-odometry information provided by the UAV platform, and formulate the surface tracking problem in a non-linear objective function that can be linearized and effectively minimized through a Gaussian-Newton method. Quantitative and qualitative experimental results demonstrate the plausible surface and motion reconstruction results.
Modulation of visual physiology by behavioral state in monkeys, mice, and flies.
Maimon, Gaby
2011-08-01
When a monkey attends to a visual stimulus, neurons in visual cortex respond differently to that stimulus than when the monkey attends elsewhere. In the 25 years since the initial discovery, the study of attention in primates has been central to understanding flexible visual processing. Recent experiments demonstrate that visual neurons in mice and fruit flies are modulated by locomotor behaviors, like running and flying, in a manner that resembles attention-based modulations in primates. The similar findings across species argue for a more generalized view of state-dependent sensory processing and for a renewed dialogue among vertebrate and invertebrate research communities. Copyright © 2011 Elsevier Ltd. All rights reserved.
Advances and limitations of visual conditioning protocols in harnessed bees.
Avarguès-Weber, Aurore; Mota, Theo
2016-10-01
Bees are excellent invertebrate models for studying visual learning and memory mechanisms, because of their sophisticated visual system and impressive cognitive capacities associated with a relatively simple brain. Visual learning in free-flying bees has been traditionally studied using an operant conditioning paradigm. This well-established protocol, however, can hardly be combined with invasive procedures for studying the neurobiological basis of visual learning. Different efforts have been made to develop protocols in which harnessed honey bees could associate visual cues with reinforcement, though learning performances remain poorer than those obtained with free-flying animals. Especially in the last decade, the intention of improving visual learning performances of harnessed bees led many authors to adopt distinct visual conditioning protocols, altering parameters like harnessing method, nature and duration of visual stimulation, number of trials, inter-trial intervals, among others. As a result, the literature provides data hardly comparable and sometimes contradictory. In the present review, we provide an extensive analysis of the literature available on visual conditioning of harnessed bees, with special emphasis on the comparison of diverse conditioning parameters adopted by different authors. Together with this comparative overview, we discuss how these diverse conditioning parameters could modulate visual learning performances of harnessed bees. Copyright © 2016 Elsevier Ltd. All rights reserved.
Effect of Surround WP on behavior and mortality of apple maggot (Diptera: Tephritidae).
Leskey, Tracy C; Wright, Starker E; Glenn, D Michael; Puterka, Gary J
2010-04-01
Apple maggot, Rhagoletis pomonella (Walsh) (Diptera: Tephritidae), is a key pest in apple (Malus spp.) production areas located in the northeastern and midwestern United States and the eastern provinces of Canada. The development of Surround WP has offered a new approach for controlling apple maggot and other tephritid species, because this material is considered to be compatible with advanced integrated pest management and organic production systems. We conducted studies aimed at identifying the behavioral and biological effects of this material on apple maggots. Specifically, we examined the effect of Surround WP on the visual ecology of adult flies under field conditions, on tactile responses of flies in semifield trials, and on fly mortality in laboratory-based-bioassays. We demonstrated that an even coating of white particles over a fruit-mimicking sphere surface reduced visual attractiveness. We also found that spotty-coated fruit-mimicking spheres (meant to mimic ripe fruit bearing an uneven coating of Surround WP) were perceived by flies as not having the ideal round silhouette shape stimulus. Surround WP served as a tactile deterrent; the residence time of females introduced on to treated fruit was much shorter compared with untreated fruit. Surround WP also had a toxic effect on both adult apple maggot and Rhagoletis suavis (Loew); flies exposed to and forced to stand on Surround-treated surfaces died in <2 d in all trials. The combined effectiveness of Surround WP is based on a reduction in the attractiveness of fruit-based visual cues, an increase in the likelihood of flies leaving treated surfaces due to tactile deterrence, and a potential for increased mortality due to exposure to Surround WP particles.
Basigin/EMMPRIN/CD147 mediates neuron-glia interactions in the optic lamina of Drosophila.
Curtin, Kathryn D; Wyman, Robert J; Meinertzhagen, Ian A
2007-11-15
Basigin, an IgG family glycoprotein found on the surface of human metastatic tumors, stimulates fibroblasts to secrete matrix metalloproteases (MMPs) that remodel the extracellular matrix, and is thus also known as Extracellular Matrix MetalloPRotease Inducer (EMMPRIN). Using Drosophila we previously identified novel roles for basigin. Specifically, photoreceptors of flies with basigin eyes show misplaced nuclei, rough ER and mitochondria, and swollen axon terminals, suggesting cytoskeletal disruptions. Here we demonstrate that basigin is required for normal neuron-glia interactions in the Drosophila visual system. Flies with basigin mutant photoreceptors have misplaced epithelial glial cells within the first optic neuropile, or lamina. In addition, epithelial glia insert finger-like projections--capitate projections (CPs)--sites of vesicle endocytosis and possibly neurotransmitter recycling. When basigin is missing from photoreceptors terminals, CP formation between glia and photoreceptor terminals is disrupted. Visual system function is also altered in flies with basigin mutant eyes. While photoreceptors depolarize normally to light, synaptic transmission is greatly diminished, consistent with a defect in neurotransmitter release. Basigin expression in photoreceptor neurons is required for normal structure and placement of glia cells.
Simple and powerful visual stimulus generator.
Kremlácek, J; Kuba, M; Kubová, Z; Vít, F
1999-02-01
We describe a cheap, simple, portable and efficient approach to visual stimulation for neurophysiology which does not need any special hardware equipment. The method based on an animation technique uses the FLI autodesk animator format. This form of the animation is replayed by a special program ('player') providing synchronisation pulses toward recording system via parallel port. The 'player is running on an IBM compatible personal computer under MS-DOS operation system and stimulus is displayed on a VGA computer monitor. Various stimuli created with this technique for visual evoked potentials (VEPs) are presented.
Conditioning to colors: a population assay for visual learning in Drosophila.
van Swinderen, Bruno
2011-11-01
Vision is a major sensory modality in Drosophila behavior, with more than one-half of the Drosophila brain devoted to visual processing. The mechanisms of vision in Drosophila can be studied in individuals and in populations of flies by using various paradigms. Although there has never been a widely used population assay for visual learning in Drosophila, some population paradigms have shown significant visual learning. These studies use colors as conditioned stimuli (CS) and shaking as the unconditioned stimulus (US). A simple version of the paradigm, conditioning to colors using a shaking device, is described here. A conditioning chamber, called a crab, is designed to center the flies after shaking by having them tumble down to the lowest point between joined glass tubes forming a V. Thus, vibration should be just strong enough to center most flies. After shaking, flies display a geotactic response and climb up either side of the V, and their choice of which side to climb is influenced by color displays on either side. The proportion of flies on either side determines the flies' natural preference or their learned avoidance of a color associated with shaking.
Vision and visual navigation in nocturnal insects.
Warrant, Eric; Dacke, Marie
2011-01-01
With their highly sensitive visual systems, nocturnal insects have evolved a remarkable capacity to discriminate colors, orient themselves using faint celestial cues, fly unimpeded through a complicated habitat, and navigate to and from a nest using learned visual landmarks. Even though the compound eyes of nocturnal insects are significantly more sensitive to light than those of their closely related diurnal relatives, their photoreceptors absorb photons at very low rates in dim light, even during demanding nocturnal visual tasks. To explain this apparent paradox, it is hypothesized that the necessary bridge between retinal signaling and visual behavior is a neural strategy of spatial and temporal summation at a higher level in the visual system. Exactly where in the visual system this summation takes place, and the nature of the neural circuitry that is involved, is currently unknown but provides a promising avenue for future research.
A lightweight, inexpensive robotic system for insect vision.
Sabo, Chelsea; Chisholm, Robert; Petterson, Adam; Cope, Alex
2017-09-01
Designing hardware for miniaturized robotics which mimics the capabilities of flying insects is of interest, because they share similar constraints (i.e. small size, low weight, and low energy consumption). Research in this area aims to enable robots with similarly efficient flight and cognitive abilities. Visual processing is important to flying insects' impressive flight capabilities, but currently, embodiment of insect-like visual systems is limited by the hardware systems available. Suitable hardware is either prohibitively expensive, difficult to reproduce, cannot accurately simulate insect vision characteristics, and/or is too heavy for small robotic platforms. These limitations hamper the development of platforms for embodiment which in turn hampers the progress on understanding of how biological systems fundamentally work. To address this gap, this paper proposes an inexpensive, lightweight robotic system for modelling insect vision. The system is mounted and tested on a robotic platform for mobile applications, and then the camera and insect vision models are evaluated. We analyse the potential of the system for use in embodiment of higher-level visual processes (i.e. motion detection) and also for development of navigation based on vision for robotics in general. Optic flow from sample camera data is calculated and compared to a perfect, simulated bee world showing an excellent resemblance. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.
UTM Technical Capabilities Level 2 (TLC2) Test at Reno-Stead Airport.
2016-10-06
Test of Unmanned Aircraft Systems Traffic Management (UTM) technical capability Level 2 (TCL2) at Reno-Stead Airport, Nevada. During the test, five drones simultaneously crossed paths, separated by altitude. Two drones flew beyond visual line-of-sight and three flew within line-of-sight of their operators. Karen Bollinger pilot and Nick Atkins of Alaska Center for Unmanned Aircraft Systems Integration program fly Ptarmigan quadcopter, one of 11 vehicles in the UTM TCL2 demonstration that will fly beyond line of sight of the pilot in command in Nevada test.
Lindemann, J P; Kern, R; Michaelis, C; Meyer, P; van Hateren, J H; Egelhaaf, M
2003-03-01
A high-speed panoramic visual stimulation device is introduced which is suitable to analyse visual interneurons during stimulation with rapid image displacements as experienced by fast moving animals. The responses of an identified motion sensitive neuron in the visual system of the blowfly to behaviourally generated image sequences are very complex and hard to predict from the established input circuitry of the neuron. This finding suggests that the computational significance of visual interneurons can only be assessed if they are characterised not only by conventional stimuli as are often used for systems analysis, but also by behaviourally relevant input.
NASA Technical Reports Server (NTRS)
Pavel, M.
1993-01-01
The topics covered include the following: a system overview of the basic components of a system designed to improve the ability of a pilot to fly through low-visibility conditions such as fog; the role of visual sciences; fusion issues; sensor characterization; sources of information; image processing; and image fusion.
Falcons pursue prey using visual motion cues: new perspectives from animal-borne cameras
Kane, Suzanne Amador; Zamani, Marjon
2014-01-01
This study reports on experiments on falcons wearing miniature videocameras mounted on their backs or heads while pursuing flying prey. Videos of hunts by a gyrfalcon (Falco rusticolus), gyrfalcon (F. rusticolus)/Saker falcon (F. cherrug) hybrids and peregrine falcons (F. peregrinus) were analyzed to determine apparent prey positions on their visual fields during pursuits. These video data were then interpreted using computer simulations of pursuit steering laws observed in insects and mammals. A comparison of the empirical and modeling data indicates that falcons use cues due to the apparent motion of prey on the falcon's visual field to track and capture flying prey via a form of motion camouflage. The falcons also were found to maintain their prey's image at visual angles consistent with using their shallow fovea. These results should prove relevant for understanding the co-evolution of pursuit and evasion, as well as the development of computer models of predation and the integration of sensory and locomotion systems in biomimetic robots. PMID:24431144
Falcons pursue prey using visual motion cues: new perspectives from animal-borne cameras.
Kane, Suzanne Amador; Zamani, Marjon
2014-01-15
This study reports on experiments on falcons wearing miniature videocameras mounted on their backs or heads while pursuing flying prey. Videos of hunts by a gyrfalcon (Falco rusticolus), gyrfalcon (F. rusticolus)/Saker falcon (F. cherrug) hybrids and peregrine falcons (F. peregrinus) were analyzed to determine apparent prey positions on their visual fields during pursuits. These video data were then interpreted using computer simulations of pursuit steering laws observed in insects and mammals. A comparison of the empirical and modeling data indicates that falcons use cues due to the apparent motion of prey on the falcon's visual field to track and capture flying prey via a form of motion camouflage. The falcons also were found to maintain their prey's image at visual angles consistent with using their shallow fovea. These results should prove relevant for understanding the co-evolution of pursuit and evasion, as well as the development of computer models of predation and the integration of sensory and locomotion systems in biomimetic robots.
Organization of the Drosophila larval visual circuit
Gendre, Nanae; Neagu-Maier, G Larisa; Fetter, Richard D; Schneider-Mizell, Casey M; Truman, James W; Zlatic, Marta; Cardona, Albert
2017-01-01
Visual systems transduce, process and transmit light-dependent environmental cues. Computation of visual features depends on photoreceptor neuron types (PR) present, organization of the eye and wiring of the underlying neural circuit. Here, we describe the circuit architecture of the visual system of Drosophila larvae by mapping the synaptic wiring diagram and neurotransmitters. By contacting different targets, the two larval PR-subtypes create two converging pathways potentially underlying the computation of ambient light intensity and temporal light changes already within this first visual processing center. Locally processed visual information then signals via dedicated projection interneurons to higher brain areas including the lateral horn and mushroom body. The stratified structure of the larval optic neuropil (LON) suggests common organizational principles with the adult fly and vertebrate visual systems. The complete synaptic wiring diagram of the LON paves the way to understanding how circuits with reduced numerical complexity control wide ranges of behaviors.
Implementation of a virtual laryngoscope system using efficient reconstruction algorithms.
Luo, Shouhua; Yan, Yuling
2009-08-01
Conventional fiberoptic laryngoscope may cause discomfort to the patient and in some cases it can lead to side effects that include perforation, infection and hemorrhage. Virtual laryngoscopy (VL) can overcome this problem and further it may lower the risk of operation failures. Very few virtual endoscope (VE) based investigations of the larynx have been described in the literature. CT data sets from a healthy subject were used for the VL studies. An algorithm of preprocessing and region-growing for 3-D image segmentation is developed. An octree based approach is applied in our VL system which facilitates a rapid construction of iso-surfaces. Some locating techniques are used for fast rendering and navigation (fly-through). Our VL visualization system provides for real time and efficient 'fly-through' navigation. The virtual camera can be arranged so that it moves along the airway in either direction. Snap shots were taken during fly-throughs. The system can automatically adjust the direction of the virtual camera and prevent collisions of the camera and the wall of the airway. A virtual laryngoscope (VL) system using OpenGL (Open Graphics Library) platform for interactive rendering and 3D visualization of the laryngeal framework and upper airway is established. OpenGL is supported on major operating systems and works with every major windowing system. The VL system runs on regular PC workstations and was successfully tested and evaluated using CT data from a normal subject.
Predator pursuit strategies: how do falcons and hawks chase prey?
NASA Astrophysics Data System (ADS)
Kane, Suzanne Amador; Zamani, Marjon; Fulton, Andrew; Rosenthal, Lee
2014-03-01
This study reports on experiments on falcons, goshawks and red-tailed hawks wearing miniature videocameras mounted on their backs or heads while pursuing flying or ground-based prey. Videos of hunts recorded by the raptors were analyzed to determine apparent prey positions on their visual fields during pursuits. These video data then were interpreted using computer simulations of pursuit steering laws observed in insects and mammals. A comparison of the empirical and modeling data indicates that falcons use cues due to the apparent motion of prey on the falcon's visual field to track and capture flying prey via a form of motion camouflage. The falcons also were found to maintain their prey's image at visual angles consistent with using their shallow fovea. Results for goshawks and red-tailed hawks were analyzed for a comparative study of how pursuits of ground-based prey by accipeters and buteos differ from those used by falcons chasing flying prey. These results should prove relevant for understanding the coevolution of pursuit and evasion, as well as the development of computer models of predation on flocks,and the integration of sensory and locomotion systems in biomimetic robots.
Flies and humans share a motion estimation strategy that exploits natural scene statistics
Clark, Damon A.; Fitzgerald, James E.; Ales, Justin M.; Gohl, Daryl M.; Silies, Marion A.; Norcia, Anthony M.; Clandinin, Thomas R.
2014-01-01
Sighted animals extract motion information from visual scenes by processing spatiotemporal patterns of light falling on the retina. The dominant models for motion estimation exploit intensity correlations only between pairs of points in space and time. Moving natural scenes, however, contain more complex correlations. Here we show that fly and human visual systems encode the combined direction and contrast polarity of moving edges using triple correlations that enhance motion estimation in natural environments. Both species extract triple correlations with neural substrates tuned for light or dark edges, and sensitivity to specific triple correlations is retained even as light and dark edge motion signals are combined. Thus, both species separately process light and dark image contrasts to capture motion signatures that can improve estimation accuracy. This striking convergence argues that statistical structures in natural scenes have profoundly affected visual processing, driving a common computational strategy over 500 million years of evolution. PMID:24390225
Hanley, M E; Cruickshanks, K L; Dunn, D; Stewart-Jones, A; Goulson, D
2009-03-01
Houseflies (Musca domestica L.) are a major pest species of livestock units and landfill sites. Insecticide resistance has resulted in an increased emphasis on lure-and-kill control methods, but the success of this approach relies on the effective attraction of houseflies with olfactory or visual stimuli. This study examined the efficacy of olfactory (cuticular hydrocarbons) or visual (colours and groups of flies) attractants in a commercial poultry unit. Despite simulating the cuticular hydrocarbon profiles of male and female houseflies, we found no significant increase in the number of individuals lured to traps and no sex-specific responses were evident. The use of target colours selected to match the three peaks in housefly visual spectral sensitivity yielded no significant increase in the catch rate of traps to which they were applied. This study also demonstrated that male and female flies possess significantly different spectral reflectance (males are brighter at 320-470 nm; females are brighter at 470-670 nm). An experiment incorporating groups of recently killed flies from which cuticular hydrocarbons were either removed by solvent or left intact also failed to show any evidence of olfactory or visual attraction for houseflies of either sex. This study concluded that variations of the most commonly applied methods of luring houseflies to traps in commercial livestock units fail to significantly increase capture rates. These results support commonly observed inconsistencies associated with using olfactory or visual stimuli in lure-and-kill systems, possibly because field conditions lessen the attractant properties observed in laboratory experiments.
Functional divisions for visual processing in the central brain of flying Drosophila
Weir, Peter T.; Dickinson, Michael H.
2015-01-01
Although anatomy is often the first step in assigning functions to neural structures, it is not always clear whether architecturally distinct regions of the brain correspond to operational units. Whereas neuroarchitecture remains relatively static, functional connectivity may change almost instantaneously according to behavioral context. We imaged panneuronal responses to visual stimuli in a highly conserved central brain region in the fruit fly, Drosophila, during flight. In one substructure, the fan-shaped body, automated analysis revealed three layers that were unresponsive in quiescent flies but became responsive to visual stimuli when the animal was flying. The responses of these regions to a broad suite of visual stimuli suggest that they are involved in the regulation of flight heading. To identify the cell types that underlie these responses, we imaged activity in sets of genetically defined neurons with arborizations in the targeted layers. The responses of this collection during flight also segregated into three sets, confirming the existence of three layers, and they collectively accounted for the panneuronal activity. Our results provide an atlas of flight-gated visual responses in a central brain circuit. PMID:26324910
Functional divisions for visual processing in the central brain of flying Drosophila.
Weir, Peter T; Dickinson, Michael H
2015-10-06
Although anatomy is often the first step in assigning functions to neural structures, it is not always clear whether architecturally distinct regions of the brain correspond to operational units. Whereas neuroarchitecture remains relatively static, functional connectivity may change almost instantaneously according to behavioral context. We imaged panneuronal responses to visual stimuli in a highly conserved central brain region in the fruit fly, Drosophila, during flight. In one substructure, the fan-shaped body, automated analysis revealed three layers that were unresponsive in quiescent flies but became responsive to visual stimuli when the animal was flying. The responses of these regions to a broad suite of visual stimuli suggest that they are involved in the regulation of flight heading. To identify the cell types that underlie these responses, we imaged activity in sets of genetically defined neurons with arborizations in the targeted layers. The responses of this collection during flight also segregated into three sets, confirming the existence of three layers, and they collectively accounted for the panneuronal activity. Our results provide an atlas of flight-gated visual responses in a central brain circuit.
A role for the membrane protein M6 in the Drosophila visual system.
Zappia, María Paula; Bernabo, Guillermo; Billi, Silvia C; Frasch, Alberto C; Ceriani, María Fernanda; Brocco, Marcela Adriana
2012-07-04
Members of the proteolipid protein family, including the four-transmembrane glycoprotein M6a, are involved in neuronal plasticity in mammals. Results from our group previously demonstrated that M6, the only proteolipid protein expressed in Drosophila, localizes to the cell membrane in follicle cells. M6 loss triggers female sterility, which suggests a role for M6 in follicular cell remodeling. These results were the basis of the present study, which focused on the function and requirements of M6 in the fly nervous system. The present study identified two novel, tissue-regulated M6 isoforms with variable N- and C- termini, and showed that M6 is the functional fly ortholog of Gpm6a. In the adult brain, the protein was localized to several neuropils, such as the optic lobe, the central complex, and the mushroom bodies. Interestingly, although reduced M6 levels triggered a mild rough-eye phenotype, hypomorphic M6 mutants exhibited a defective response to light. Based on its ability to induce filopodium formation we propose that M6 is key in cell remodeling processes underlying visual system function. These results bring further insight into the role of M6/M6a in biological processes involving neuronal plasticity and behavior in flies and mammals.
A role for the membrane protein M6 in the Drosophila visual system
2012-01-01
Background Members of the proteolipid protein family, including the four-transmembrane glycoprotein M6a, are involved in neuronal plasticity in mammals. Results from our group previously demonstrated that M6, the only proteolipid protein expressed in Drosophila, localizes to the cell membrane in follicle cells. M6 loss triggers female sterility, which suggests a role for M6 in follicular cell remodeling. These results were the basis of the present study, which focused on the function and requirements of M6 in the fly nervous system. Results The present study identified two novel, tissue-regulated M6 isoforms with variable N- and C- termini, and showed that M6 is the functional fly ortholog of Gpm6a. In the adult brain, the protein was localized to several neuropils, such as the optic lobe, the central complex, and the mushroom bodies. Interestingly, although reduced M6 levels triggered a mild rough-eye phenotype, hypomorphic M6 mutants exhibited a defective response to light. Conclusions Based on its ability to induce filopodium formation we propose that M6 is key in cell remodeling processes underlying visual system function. These results bring further insight into the role of M6/M6a in biological processes involving neuronal plasticity and behavior in flies and mammals. PMID:22762289
Modeling a Million-Node Slim Fly Network Using Parallel Discrete-Event Simulation
DOE Office of Scientific and Technical Information (OSTI.GOV)
Wolfe, Noah; Carothers, Christopher; Mubarak, Misbah
As supercomputers close in on exascale performance, the increased number of processors and processing power translates to an increased demand on the underlying network interconnect. The Slim Fly network topology, a new lowdiameter and low-latency interconnection network, is gaining interest as one possible solution for next-generation supercomputing interconnect systems. In this paper, we present a high-fidelity Slim Fly it-level model leveraging the Rensselaer Optimistic Simulation System (ROSS) and Co-Design of Exascale Storage (CODES) frameworks. We validate our Slim Fly model with the Kathareios et al. Slim Fly model results provided at moderately sized network scales. We further scale the modelmore » size up to n unprecedented 1 million compute nodes; and through visualization of network simulation metrics such as link bandwidth, packet latency, and port occupancy, we get an insight into the network behavior at the million-node scale. We also show linear strong scaling of the Slim Fly model on an Intel cluster achieving a peak event rate of 36 million events per second using 128 MPI tasks to process 7 billion events. Detailed analysis of the underlying discrete-event simulation performance shows that a million-node Slim Fly model simulation can execute in 198 seconds on the Intel cluster.« less
Visual responses of corn silk flies (Diptera: Ulidiidae)
USDA-ARS?s Scientific Manuscript database
Corn silk flies are major pests impacting fresh market sweet corn production in Florida and Georgia. Control depends solely on well-times applications of insecticides to protect corn ear development. Surveillance depends on visual inspection of ears with no effective trapping methods currently ava...
1987-09-01
Visual Communication . Although this task is performed several times, the task is performed at different points during the mission. In addition, the...Perform visual communication Give thumbs-up signal when ready for takeoff; check lights on pri-fly B. Perform takeoff and Aircraft operating clear ship...FM c. Operate ICS 2. Perform visual communication 3. Operate IFF transponder B. Maintain mission and fuel logs C. Perform checklists 1. Perform AMCM
NASA Astrophysics Data System (ADS)
Duong, Tuan A.; Duong, Nghi; Le, Duong
2017-01-01
In this paper, we present an integration technique using a bio-inspired, control-based visual and olfactory receptor system to search for elusive targets in practical environments where the targets cannot be seen obviously by either sensory data. Bio-inspired Visual System is based on a modeling of extended visual pathway which consists of saccadic eye movements and visual pathway (vertebrate retina, lateral geniculate nucleus and visual cortex) to enable powerful target detections of noisy, partial, incomplete visual data. Olfactory receptor algorithm, namely spatial invariant independent component analysis, that was developed based on data of old factory receptor-electronic nose (enose) of Caltech, is adopted to enable the odorant target detection in an unknown environment. The integration of two systems is a vital approach and sets up a cornerstone for effective and low-cost of miniaturized UAVs or fly robots for future DOD and NASA missions, as well as for security systems in Internet of Things environments.
Code of Federal Regulations, 2012 CFR
2012-01-01
... pilots flying under visual flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME... flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME. (a) Operations within a 60-nautical mile radius of the Washington, DC VOR/DME under visual flight rules (VFR). Except as provided...
Code of Federal Regulations, 2011 CFR
2011-01-01
... pilots flying under visual flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME... flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME. (a) Operations within a 60-nautical mile radius of the Washington, DC VOR/DME under visual flight rules (VFR). Except as provided...
Code of Federal Regulations, 2013 CFR
2013-01-01
... pilots flying under visual flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME... flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME. (a) Operations within a 60-nautical mile radius of the Washington, DC VOR/DME under visual flight rules (VFR). Except as provided...
Code of Federal Regulations, 2014 CFR
2014-01-01
... pilots flying under visual flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME... flight rules within a 60-nautical mile radius of the Washington, DC VOR/DME. (a) Operations within a 60-nautical mile radius of the Washington, DC VOR/DME under visual flight rules (VFR). Except as provided...
J-FLiC UAS Flights for Acoustic Testing Research
NASA Technical Reports Server (NTRS)
Motter, Mark A.; High, James W.
2016-01-01
The jet-powered flying testbed (J-FLiC) unmanned aircraft system (UAS) successfully completed twenty-six flights at Fort AP Hill, VA, from 27 August until September 3 2015, supporting tests of a microphone array system for aircraft noise measurement. The test vehicles, J-FLiC NAVY2 (N508NU), and J-FLiC 4 (N509NU), were flown under manual and autopiloted control in a variety of test conditions: clean at speeds ranging from 80 to 150 knots; and full landing configuration at speeds ranging from 50 to 95 knots. During the test campaign, autopilot capability was incrementally improved to ultimately provide a high degree of accuracy and repeatability of the critical test requirements for airspeed, altitude, runway alignment and position over the microphone array. Manual flights were performed for test conditions at the both ends of the speed envelope where autopiloted flight would have required flight beyond visual range and more extensive developmental work. The research objectives of the campaign were fully achieved. The ARMD Integrated Systems Research Program (ISRP) Environmentally Responsible Aviation (ERA) Project aims to develop the enabling capabilities/technologies that will allow prediction/reduction of aircraft noise. A primary measurement tool for ascertaining and characterizing empirically the effectiveness of various noise reduction technologies is a microphone phased array system. Such array systems need to be vetted and certified for operational use via field deployments and overflights of the array with test aircraft, in this case with sUAS aircraft such as J-FLiC.
2006-06-05
Space shuttle STS-121 FIT (Fly Immunity and Tumors) payload. Using Drosophila (fruit fly) to complete the experiments. Computer screen showing green fluorescent protein used to visualize blood cells in Drosophila (fruit fly).
Case-Based Behavior Recognition in Beyond Visual Range Air Combat
2015-05-01
Auslander et al. 2014), which can then be given to a plan generator . Recognizing high-level behaviors, which is our focus, should also help to recognize...kinematically avoid a missile by flying away from it), and Crank (an agent flies at the maximum offset but tries to keep its target in radar). For the UAV and...3.4 the pruning algorithm must take into account the limitations of the acquisition system, which generally results in All Out Aggressive cases being
2014-03-16
College in the Department of Bioengineering with: Dr Martyn Boutelle (Biosensors), Dr Simon Schultz (Neural Coding), Dr Manos Drakakis (Low-power VLSI...in the fly gaze stabilization system. PLoS Biology, 6(7):e173 (2008). O27. Saleem A, Krapp H.G., and Schultz S.R.: Spike-triggered independent...D., Krapp H.G., Schultz S.R.: Bimodal optomotor response to plaids in blowflies: mechanisms of component selectivity and evidence for pattern
1976-01-01
Center (NAFEC), the National Aeronautics and Space Administration (NASA), the Boeing Company , and the United States Air Force. The airplanes involved...Establishment (RAE) in England, reference 28, and the Boeing Company , reference 2, revealed good correlation in vortex persistence with tower fly-by...AeronauticalI Research Council, London, Paper No. 16,666, 1968. 21. Schlichting, H., Boundary Layer Theory, Fourth Edition, McGraw-Hill Book Company , New York
Nocturnal insects use optic flow for flight control
Baird, Emily; Kreiss, Eva; Wcislo, William; Warrant, Eric; Dacke, Marie
2011-01-01
To avoid collisions when navigating through cluttered environments, flying insects must control their flight so that their sensory systems have time to detect obstacles and avoid them. To do this, day-active insects rely primarily on the pattern of apparent motion generated on the retina during flight (optic flow). However, many flying insects are active at night, when obtaining reliable visual information for flight control presents much more of a challenge. To assess whether nocturnal flying insects also rely on optic flow cues to control flight in dim light, we recorded flights of the nocturnal neotropical sweat bee, Megalopta genalis, flying along an experimental tunnel when: (i) the visual texture on each wall generated strong horizontal (front-to-back) optic flow cues, (ii) the texture on only one wall generated these cues, and (iii) horizontal optic flow cues were removed from both walls. We find that Megalopta increase their groundspeed when horizontal motion cues in the tunnel are reduced (conditions (ii) and (iii)). However, differences in the amount of horizontal optic flow on each wall of the tunnel (condition (ii)) do not affect the centred position of the bee within the flight tunnel. To better understand the behavioural response of Megalopta, we repeated the experiments on day-active bumble-bees (Bombus terrestris). Overall, our findings demonstrate that despite the limitations imposed by dim light, Megalopta—like their day-active relatives—rely heavily on vision to control flight, but that they use visual cues in a different manner from diurnal insects. PMID:21307047
Behavioural system identification of visual flight speed control in Drosophila melanogaster
Rohrseitz, Nicola; Fry, Steven N.
2011-01-01
Behavioural control in many animals involves complex mechanisms with intricate sensory-motor feedback loops. Modelling allows functional aspects to be captured without relying on a description of the underlying complex, and often unknown, mechanisms. A wide range of engineering techniques are available for modelling, but their ability to describe time-continuous processes is rarely exploited to describe sensory-motor control mechanisms in biological systems. We performed a system identification of visual flight speed control in the fruitfly Drosophila, based on an extensive dataset of open-loop responses previously measured under free flight conditions. We identified a second-order under-damped control model with just six free parameters that well describes both the transient and steady-state characteristics of the open-loop data. We then used the identified control model to predict flight speed responses after a visual perturbation under closed-loop conditions and validated the model with behavioural measurements performed in free-flying flies under the same closed-loop conditions. Our system identification of the fruitfly's flight speed response uncovers the high-level control strategy of a fundamental flight control reflex without depending on assumptions about the underlying physiological mechanisms. The results are relevant for future investigations of the underlying neuromotor processing mechanisms, as well as for the design of biomimetic robots, such as micro-air vehicles. PMID:20525744
Behavioural system identification of visual flight speed control in Drosophila melanogaster.
Rohrseitz, Nicola; Fry, Steven N
2011-02-06
Behavioural control in many animals involves complex mechanisms with intricate sensory-motor feedback loops. Modelling allows functional aspects to be captured without relying on a description of the underlying complex, and often unknown, mechanisms. A wide range of engineering techniques are available for modelling, but their ability to describe time-continuous processes is rarely exploited to describe sensory-motor control mechanisms in biological systems. We performed a system identification of visual flight speed control in the fruitfly Drosophila, based on an extensive dataset of open-loop responses previously measured under free flight conditions. We identified a second-order under-damped control model with just six free parameters that well describes both the transient and steady-state characteristics of the open-loop data. We then used the identified control model to predict flight speed responses after a visual perturbation under closed-loop conditions and validated the model with behavioural measurements performed in free-flying flies under the same closed-loop conditions. Our system identification of the fruitfly's flight speed response uncovers the high-level control strategy of a fundamental flight control reflex without depending on assumptions about the underlying physiological mechanisms. The results are relevant for future investigations of the underlying neuromotor processing mechanisms, as well as for the design of biomimetic robots, such as micro-air vehicles.
NASA Technical Reports Server (NTRS)
Grantham, William D.; Person, Lee H., Jr.; Brown, Philip W.; Becker, Lawrence E.; Hunt, George E.; Rising, J. J.; Davis, W. J.; Willey, C. S.; Weaver, W. A.; Cokeley, R.
1985-01-01
Piloted simulation studies have been conducted to evaluate the effectiveness of two pitch active control systems (PACS) on the flying qualities of a wide-body transport airplane when operating at negative static margins. These two pitch active control systems consisted of a simple 'near-term' PACS and a more complex 'advanced' PACS. Eight different flight conditions, representing the entire flight envelope, were evaluated with emphasis on the cruise flight conditions. These studies were made utilizing the Langley Visual/Motion Simulator (VMS) which has six degrees of freedom. The simulation tests indicated that (1) the flying qualities of the baseline aircraft (PACS off) for the cruise and other high-speed flight conditions were unacceptable at center-of-gravity positions aft of the neutral static stability point; (2) within the linear static stability flight envelope, the near-term PACS provided acceptable flying qualities for static stabilty margins to -3 percent; and (3) with the advanced PACS operative, the flying qualities were demonstrated to be good (satisfactory to very acceptable) for static stabilty margins to -20 percent.
Eye evolution at high resolution: the neuron as a unit of homology.
Erclik, Ted; Hartenstein, Volker; McInnes, Roderick R; Lipshitz, Howard D
2009-08-01
Based on differences in morphology, photoreceptor-type usage and lens composition it has been proposed that complex eyes have evolved independently many times. The remarkable observation that different eye types rely on a conserved network of genes (including Pax6/eyeless) for their formation has led to the revised proposal that disparate complex eye types have evolved from a shared and simpler prototype. Did this ancestral eye already contain the neural circuitry required for image processing? And what were the evolutionary events that led to the formation of complex visual systems, such as those found in vertebrates and insects? The recent identification of unexpected cell-type homologies between neurons in the vertebrate and Drosophila visual systems has led to two proposed models for the evolution of complex visual systems from a simple prototype. The first, as an extension of the finding that the neurons of the vertebrate retina share homologies with both insect (rhabdomeric) and vertebrate (ciliary) photoreceptor cell types, suggests that the vertebrate retina is a composite structure, made up of neurons that have evolved from two spatially separate ancestral photoreceptor populations. The second model, based largely on the conserved role for the Vsx homeobox genes in photoreceptor-target neuron development, suggests that the last common ancestor of vertebrates and flies already possessed a relatively sophisticated visual system that contained a mixture of rhabdomeric and ciliary photoreceptors as well as their first- and second-order target neurons. The vertebrate retina and fly visual system would have subsequently evolved by elaborating on this ancestral neural circuit. Here we present evidence for these two cell-type homology-based models and discuss their implications.
Associative visual learning by tethered bees in a controlled visual environment.
Buatois, Alexis; Pichot, Cécile; Schultheiss, Patrick; Sandoz, Jean-Christophe; Lazzari, Claudio R; Chittka, Lars; Avarguès-Weber, Aurore; Giurfa, Martin
2017-10-10
Free-flying honeybees exhibit remarkable cognitive capacities but the neural underpinnings of these capacities cannot be studied in flying insects. Conversely, immobilized bees are accessible to neurobiological investigation but display poor visual learning. To overcome this limitation, we aimed at establishing a controlled visual environment in which tethered bees walking on a spherical treadmill learn to discriminate visual stimuli video projected in front of them. Freely flying bees trained to walk into a miniature Y-maze displaying these stimuli in a dark environment learned the visual discrimination efficiently when one of them (CS+) was paired with sucrose and the other with quinine solution (CS-). Adapting this discrimination to the treadmill paradigm with a tethered, walking bee was successful as bees exhibited robust discrimination and preferred the CS+ to the CS- after training. As learning was better in the maze, movement freedom, active vision and behavioral context might be important for visual learning. The nature of the punishment associated with the CS- also affects learning as quinine and distilled water enhanced the proportion of learners. Thus, visual learning is amenable to a controlled environment in which tethered bees learn visual stimuli, a result that is important for future neurobiological studies in virtual reality.
The role of vision in odor-plume tracking by walking and flying insects.
Willis, Mark A; Avondet, Jennifer L; Zheng, Elizabeth
2011-12-15
The walking paths of male cockroaches, Periplaneta americana, tracking point-source plumes of female pheromone often appear similar in structure to those observed from flying male moths. Flying moths use visual-flow-field feedback of their movements to control steering and speed over the ground and to detect the wind speed and direction while tracking plumes of odors. Walking insects are also known to use flow field cues to steer their trajectories. Can the upwind steering we observe in plume-tracking walking male cockroaches be explained by visual-flow-field feedback, as in flying moths? To answer this question, we experimentally occluded the compound eyes and ocelli of virgin P. americana males, separately and in combination, and challenged them with different wind and odor environments in our laboratory wind tunnel. They were observed responding to: (1) still air and no odor, (2) wind and no odor, (3) a wind-borne point-source pheromone plume and (4) a wide pheromone plume in wind. If walking cockroaches require visual cues to control their steering with respect to their environment, we would expect their tracks to be less directed and more variable if they cannot see. Instead, we found few statistically significant differences among behaviors exhibited by intact control cockroaches or those with their eyes occluded, under any of our environmental conditions. Working towards our goal of a comprehensive understanding of chemo-orientation in insects, we then challenged flying and walking male moths to track pheromone plumes with and without visual feedback. Neither walking nor flying moths performed as well as walking cockroaches when there was no visual information available.
The role of vision in odor-plume tracking by walking and flying insects
Willis, Mark A.; Avondet, Jennifer L.; Zheng, Elizabeth
2011-01-01
SUMMARY The walking paths of male cockroaches, Periplaneta americana, tracking point-source plumes of female pheromone often appear similar in structure to those observed from flying male moths. Flying moths use visual-flow-field feedback of their movements to control steering and speed over the ground and to detect the wind speed and direction while tracking plumes of odors. Walking insects are also known to use flow field cues to steer their trajectories. Can the upwind steering we observe in plume-tracking walking male cockroaches be explained by visual-flow-field feedback, as in flying moths? To answer this question, we experimentally occluded the compound eyes and ocelli of virgin P. americana males, separately and in combination, and challenged them with different wind and odor environments in our laboratory wind tunnel. They were observed responding to: (1) still air and no odor, (2) wind and no odor, (3) a wind-borne point-source pheromone plume and (4) a wide pheromone plume in wind. If walking cockroaches require visual cues to control their steering with respect to their environment, we would expect their tracks to be less directed and more variable if they cannot see. Instead, we found few statistically significant differences among behaviors exhibited by intact control cockroaches or those with their eyes occluded, under any of our environmental conditions. Working towards our goal of a comprehensive understanding of chemo-orientation in insects, we then challenged flying and walking male moths to track pheromone plumes with and without visual feedback. Neither walking nor flying moths performed as well as walking cockroaches when there was no visual information available. PMID:22116754
Human factor roles in design of teleoperator systems
NASA Technical Reports Server (NTRS)
Janow, C.; Malone, T. B.
1973-01-01
Teleoperator systems are considered, giving attention to types of teleoperators, a manned space vehicle attached manipulator, a free-flying teleoperator, a surface exploration roving vehicle, the human factors role in total system design, the manipulator system, the sensor system, the communication system, the control system, and the mobility system. The role of human factors in the development of teleoperator systems is also discussed, taking into account visual systems, an operator control station, and the manipulators.
Visual control of prey-capture flight in dragonflies.
Olberg, Robert M
2012-04-01
Interacting with a moving object poses a computational problem for an animal's nervous system. This problem has been elegantly solved by the dragonfly, a formidable visual predator on flying insects. The dragonfly computes an interception flight trajectory and steers to maintain it during its prey-pursuit flight. This review summarizes current knowledge about pursuit behavior and neurons thought to control interception in the dragonfly. When understood, this system has the potential for explaining how a small group of neurons can control complex interactions with moving objects. Copyright © 2011 Elsevier Ltd. All rights reserved.
Testing the FLI in the region of the Pallas asteroid family
NASA Astrophysics Data System (ADS)
Todorović, N.; Novaković, B.
2015-08-01
Computation of the fast Lyapunov indicator (FLI) is one of the most efficient numerical ways to characterize dynamical nature of motion and to detect phase-space structures in a large variety of dynamical models. Despite its effectiveness, FLI was mainly used for symplectic maps or simple Hamiltonians, but it has never been used to study dynamics of asteroids to a greater extent. This research shows that FLI could also be successfully applied to real (Solar system) dynamics. For this purpose, we focus on the main belt region where the Pallas asteroid family is located. By using the full Solar system model, different sets of initial conditions and different integration times, we managed not only to visualize a large multiplet of resonances located in the region, but also their structures, chaotic boundaries, stability islands therein and the positions of their mutual interaction. In the end, we have identified some of the most dominant resonances present in the region and established a link between these resonances and chaotic areas visible in our maps. We have illustrated that FLI once again has shown its efficiency to detect dynamical structures in the main belt, e.g. in the Pallas asteroid family, with a surprisingly good clarity.
Advanced Technology for Portable Personal Visualization
1991-12-01
sites. VPL Research began in 1989 selling0IDapitP incodaesyemW commemcillys a KD system that used a glove to control the actions of flying and grabbing...problem of beacon switching error or its equivalent . Steps we took to control these errors would apply to other (3) Ascension Technology Corporation. The...AD-A245 905 / /7 Advanced Technology for Portable Personal Visualization I) ICReport of Research Progress JAN 3.ELEC April - December 1991I ELECTE I
Nocturnal insects use optic flow for flight control.
Baird, Emily; Kreiss, Eva; Wcislo, William; Warrant, Eric; Dacke, Marie
2011-08-23
To avoid collisions when navigating through cluttered environments, flying insects must control their flight so that their sensory systems have time to detect obstacles and avoid them. To do this, day-active insects rely primarily on the pattern of apparent motion generated on the retina during flight (optic flow). However, many flying insects are active at night, when obtaining reliable visual information for flight control presents much more of a challenge. To assess whether nocturnal flying insects also rely on optic flow cues to control flight in dim light, we recorded flights of the nocturnal neotropical sweat bee, Megalopta genalis, flying along an experimental tunnel when: (i) the visual texture on each wall generated strong horizontal (front-to-back) optic flow cues, (ii) the texture on only one wall generated these cues, and (iii) horizontal optic flow cues were removed from both walls. We find that Megalopta increase their groundspeed when horizontal motion cues in the tunnel are reduced (conditions (ii) and (iii)). However, differences in the amount of horizontal optic flow on each wall of the tunnel (condition (ii)) do not affect the centred position of the bee within the flight tunnel. To better understand the behavioural response of Megalopta, we repeated the experiments on day-active bumble-bees (Bombus terrestris). Overall, our findings demonstrate that despite the limitations imposed by dim light, Megalopta-like their day-active relatives-rely heavily on vision to control flight, but that they use visual cues in a different manner from diurnal insects. This journal is © 2011 The Royal Society
Nonlinear circuits for naturalistic visual motion estimation
Fitzgerald, James E; Clark, Damon A
2015-01-01
Many animals use visual signals to estimate motion. Canonical models suppose that animals estimate motion by cross-correlating pairs of spatiotemporally separated visual signals, but recent experiments indicate that humans and flies perceive motion from higher-order correlations that signify motion in natural environments. Here we show how biologically plausible processing motifs in neural circuits could be tuned to extract this information. We emphasize how known aspects of Drosophila's visual circuitry could embody this tuning and predict fly behavior. We find that segregating motion signals into ON/OFF channels can enhance estimation accuracy by accounting for natural light/dark asymmetries. Furthermore, a diversity of inputs to motion detecting neurons can provide access to more complex higher-order correlations. Collectively, these results illustrate how non-canonical computations improve motion estimation with naturalistic inputs. This argues that the complexity of the fly's motion computations, implemented in its elaborate circuits, represents a valuable feature of its visual motion estimator. DOI: http://dx.doi.org/10.7554/eLife.09123.001 PMID:26499494
"The Flying Man": The Power of Visual Media in Social Education
ERIC Educational Resources Information Center
Pearcy, Mark
2015-01-01
The visual arts and media historically employed by teachers as a corollary to "traditional" social education can play a more vital role in promoting critical inquiry among students. The use of short films like "The Flying Man" (2013), a 2013 motion picture which depicts an almost mythic vigilante in a realistic world, can help…
Murillo-Maldonado, Juan M.; Sánchez-Chávez, Gustavo; Salgado, Luis M.; Salceda, Rocío; Riesgo-Escovar, Juan R.
2011-01-01
OBJECTIVE Type 2 diabetes is the most common form of diabetes worldwide. Some of its complications, such as retinopathy and neuropathy, are long-term and protracted, with an unclear etiology. Given this problem, genetic model systems, such as in flies where type 2 diabetes can be modeled and studied, offer distinct advantages. RESEARCH DESIGN AND METHODS We used individual flies in experiments: control and mutant individuals with partial loss-of-function insulin pathway genes. We measured wing size and tested body weight for growth phenotypes, the latter by means of a microbalance. We studied total lipid and carbohydrate content, lipids by a reaction in single fly homogenates with vanillin-phosphoric acid, and carbohydrates with an anthrone-sulfuric acid reaction. Cholinesterase activity was measured using the Ellman method in head homogenates from pooled fly heads, and electroretinograms with glass capillary microelectrodes to assess performance of central brain activity and retinal function. RESULTS Flies with partial loss-of-function of insulin pathway genes have significantly reduced body weight, higher total lipid content, and sometimes elevated carbohydrate levels. Brain function is impaired, as is retinal function, but no clear correlation can be drawn from nervous system function and metabolic state. CONCLUSIONS These studies show that flies can be models of type 2 diabetes. They weigh less but have significant lipid gains (obese); some also have carbohydrate gains and compromised brain and retinal functions. This is significant because flies have an open circulatory system without microvasculature and can be studied without the complications of vascular defects. PMID:21464442
Kress, Daniel; Egelhaaf, Martin
2014-01-01
During locomotion animals rely heavily on visual cues gained from the environment to guide their behavior. Examples are basic behaviors like collision avoidance or the approach to a goal. The saccadic gaze strategy of flying flies, which separates translational from rotational phases of locomotion, has been suggested to facilitate the extraction of environmental information, because only image flow evoked by translational self-motion contains relevant distance information about the surrounding world. In contrast to the translational phases of flight during which gaze direction is kept largely constant, walking flies experience continuous rotational image flow that is coupled to their stride-cycle. The consequences of these self-produced image shifts for the extraction of environmental information are still unclear. To assess the impact of stride-coupled image shifts on visual information processing, we performed electrophysiological recordings from the HSE cell, a motion sensitive wide-field neuron in the blowfly visual system. This cell has been concluded to play a key role in mediating optomotor behavior, self-motion estimation and spatial information processing. We used visual stimuli that were based on the visual input experienced by walking blowflies while approaching a black vertical bar. The response of HSE to these stimuli was dominated by periodic membrane potential fluctuations evoked by stride-coupled image shifts. Nevertheless, during the approach the cell’s response contained information about the bar and its background. The response components evoked by the bar were larger than the responses to its background, especially during the last phase of the approach. However, as revealed by targeted modifications of the visual input during walking, the extraction of distance information on the basis of HSE responses is much impaired by stride-coupled retinal image shifts. Possible mechanisms that may cope with these stride-coupled responses are discussed. PMID:25309362
Multi-camera real-time three-dimensional tracking of multiple flying animals
Straw, Andrew D.; Branson, Kristin; Neumann, Titus R.; Dickinson, Michael H.
2011-01-01
Automated tracking of animal movement allows analyses that would not otherwise be possible by providing great quantities of data. The additional capability of tracking in real time—with minimal latency—opens up the experimental possibility of manipulating sensory feedback, thus allowing detailed explorations of the neural basis for control of behaviour. Here, we describe a system capable of tracking the three-dimensional position and body orientation of animals such as flies and birds. The system operates with less than 40 ms latency and can track multiple animals simultaneously. To achieve these results, a multi-target tracking algorithm was developed based on the extended Kalman filter and the nearest neighbour standard filter data association algorithm. In one implementation, an 11-camera system is capable of tracking three flies simultaneously at 60 frames per second using a gigabit network of nine standard Intel Pentium 4 and Core 2 Duo computers. This manuscript presents the rationale and details of the algorithms employed and shows three implementations of the system. An experiment was performed using the tracking system to measure the effect of visual contrast on the flight speed of Drosophila melanogaster. At low contrasts, speed is more variable and faster on average than at high contrasts. Thus, the system is already a useful tool to study the neurobiology and behaviour of freely flying animals. If combined with other techniques, such as ‘virtual reality’-type computer graphics or genetic manipulation, the tracking system would offer a powerful new way to investigate the biology of flying animals. PMID:20630879
Pilot response to peripheral vision cues during instrument flying tasks.
DOT National Transportation Integrated Search
1968-02-01
In an attempt to more closely associate the visual aspects of instrument flying with that of contact flight, a study was made of human response to peripheral vision cues relating to aircraft roll attitude. Pilots, ranging from 52 to 12,000 flying hou...
1992-01-01
results in stimulation of spatial-motion-location visual processes, which are known to take precedence over any other sensor or cognitive stimuli. In...or version he is flying. This was initially an observation that stimulated the birth of the human-factors engineering discipline during World War H...collisions with the surface, the pilot needs inputs to sensory channels other than the focal visual system. Properly designed auditory and
Policha, Tobias; Davis, Aleah; Barnadas, Melinda; Dentinger, Bryn T M; Raguso, Robert A; Roy, Bitty A
2016-05-01
Flowers use olfactory and visual signals to communicate with pollinators. Disentangling the relative contributions and potential synergies between signals remains a challenge. Understanding the perceptual biases exploited by floral mimicry illuminates the evolution of these signals. Here, we disentangle the olfactory and visual components of Dracula lafleurii, which mimics mushrooms in size, shape, color and scent, and is pollinated by mushroom-associated flies. To decouple signals, we used three-dimensional printing to produce realistic artificial flower molds that were color matched and cast using scent-free surgical silicone, to which we could add scent. We used GC-MS to measure scents in co-occurring mushrooms, and related orchids, and used these scents in field experiments. By combining silicone flower parts with real floral organs, we created chimeras that identified the mushroom-like labellum as a source of volatile attraction. In addition, we showed remarkable overlap in the volatile chemistry between D. lafleurii and co-occurring mushrooms. The characters defining the genus Dracula - a mushroom-like, 'gilled' labellum and a showy, patterned calyx - enhance pollinator attraction by exploiting the visual and chemosensory perceptual biases of drosophilid flies. Our techniques for the manipulation of complex traits in a nonmodel system not conducive to gene silencing or selective breeding are useful for other systems. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.
Using Cascading Style Sheets to Design a Fly-Out Menu with Microsoft Visual Studio
ERIC Educational Resources Information Center
Liu, Chang; Downing, Charles
2010-01-01
The menu has become an integrated component within nearly all professionally designed websites. This teaching tip presents a no-code way to design either a vertical or a horizontal fly-out menu by using Cascading Style Sheets (CSS) within Microsoft Visual Studio 2008. The approach described in this tip helps students fully understand how to…
Visual discrimination transfer and modulation by biogenic amines in honeybees.
Vieira, Amanda Rodrigues; Salles, Nayara; Borges, Marco; Mota, Theo
2018-05-10
For more than a century, visual learning and memory have been studied in the honeybee Apis mellifera using operant appetitive conditioning. Although honeybees show impressive visual learning capacities in this well-established protocol, operant training of free-flying animals cannot be combined with invasive protocols for studying the neurobiological basis of visual learning. In view of this, different attempts have been made to develop new classical conditioning protocols for studying visual learning in harnessed honeybees, though learning performance remains considerably poorer than that for free-flying animals. Here, we investigated the ability of honeybees to use visual information acquired during classical conditioning in a new operant context. We performed differential visual conditioning of the proboscis extension reflex (PER) followed by visual orientation tests in a Y-maze. Classical conditioning and Y-maze retention tests were performed using the same pair of perceptually isoluminant chromatic stimuli, to avoid the influence of phototaxis during free-flying orientation. Visual discrimination transfer was clearly observed, with pre-trained honeybees significantly orienting their flights towards the former positive conditioned stimulus (CS+), thus showing that visual memories acquired by honeybees are resistant to context changes between conditioning and the retention test. We combined this visual discrimination approach with selective pharmacological injections to evaluate the effect of dopamine and octopamine in appetitive visual learning. Both octopaminergic and dopaminergic antagonists impaired visual discrimination performance, suggesting that both these biogenic amines modulate appetitive visual learning in honeybees. Our study brings new insight into cognitive and neurobiological mechanisms underlying visual learning in honeybees. © 2018. Published by The Company of Biologists Ltd.
Augmented Reality Imaging System: 3D Viewing of a Breast Cancer.
Douglas, David B; Boone, John M; Petricoin, Emanuel; Liotta, Lance; Wilson, Eugene
2016-01-01
To display images of breast cancer from a dedicated breast CT using Depth 3-Dimensional (D3D) augmented reality. A case of breast cancer imaged using contrast-enhanced breast CT (Computed Tomography) was viewed with the augmented reality imaging, which uses a head display unit (HDU) and joystick control interface. The augmented reality system demonstrated 3D viewing of the breast mass with head position tracking, stereoscopic depth perception, focal point convergence and the use of a 3D cursor and joy-stick enabled fly through with visualization of the spiculations extending from the breast cancer. The augmented reality system provided 3D visualization of the breast cancer with depth perception and visualization of the mass's spiculations. The augmented reality system should be further researched to determine the utility in clinical practice.
Britten, Kenneth H.; Thatcher, Timothy D.; Caro, Tim
2016-01-01
Experimental and comparative evidence suggests that the striped coats of zebras deter biting fly attack, but the mechanisms by which flies fail to target black-and-white mammals are still opaque. Two hypotheses have been proposed: stripes might serve either to defeat polarotaxis or to obscure the form of the animal. To test these hypotheses, we systematically photographed free-living plains zebras in Africa. We found that black and white stripes both have moderate polarization signatures with a similar angle, though the degree (magnitude) of polarization in white stripes is lower. When we modeled the visibility of these signals from different distances, we found that polarization differences between stripes are invisible to flies more than 10 m away because they are averaged out by the flies’ low visual resolution. At any distance, however, a positively polarotactic insect would have a distinct signal to guide its visual approach to a zebra because we found that polarization of light reflecting from zebras is higher than from surrounding dry grasses. We also found that the stripes themselves are visible to flies at somewhat greater distances (up to 20 m) than the polarization contrast between stripes. Together, these observations support hypotheses in which zebra stripes defeat visually guided orienting behavior in flies by a mechanism independent of polarotaxis. PMID:27223616
NASA Astrophysics Data System (ADS)
Hanhart, Philippe; Ebrahimi, Touradj
2014-03-01
Crosstalk and vergence-accommodation rivalry negatively impact the quality of experience (QoE) provided by stereoscopic displays. However, exploiting visual attention and adapting the 3D rendering process on the fly can reduce these drawbacks. In this paper, we propose and evaluate two different approaches that exploit visual attention to improve 3D QoE on stereoscopic displays: an offline system, which uses a saliency map to predict gaze position, and an online system, which uses a remote eye tracking system to measure real time gaze positions. The gaze points were used in conjunction with the disparity map to extract the disparity of the object-of-interest. Horizontal image translation was performed to bring the fixated object on the screen plane. The user preference between standard 3D mode and the two proposed systems was evaluated through a subjective evaluation. Results show that exploiting visual attention significantly improves image quality and visual comfort, with a slight advantage for real time gaze determination. Depth quality is also improved, but the difference is not significant.
Davidson, Melanie M; Butler, Ruth C; Teulon, David A J
2006-07-01
The effects of starvation or age on the walking or flying response of female Frankliniella occidentalis to visual and/or odor cues in two types of olfactometer were examined in the laboratory. The response of walking thrips starved for 0, 1, 4, or 24h to an odor cue (1microl of 10% p-anisaldehyde) was examined in a Y-tube olfactometer. The take-off and landing response of thrips (unknown age) starved for 0, 1, 4, 24, 48 or 72h, or of thrips of different ages (2-3 days or 10-13 days post-adult emergence) starved for 24h, to a visual cue (98 cm(2) yellow sticky trap) and/or an odor cue (0.5 or 1.0 ml p-anisaldehyde) was examined in a wind tunnel. More thrips walked up the odor-laden arm in the Y-tube when starved for at least 4h (76%) than satiated thrips (58.7%) or those starved for 1h (62.7%, P<0.05). In the wind tunnel experiments the percentage of thrips to fly or land on the sticky trap increased between satiated thrips (7.3% to fly, 3.3% on trap) and those starved for 4h (81.2% to fly, 29% on trap) and decreased between thrips starved for 48 (74.5% to fly, 23% on trap) and 72 h (56.5% to fly, 15.5% on trap, P<0.05). Fewer younger thrips (38.8%) landed on a sticky trap containing a yellow visual cue of, those that flew, than older thrips (70.4%, P<0.05), although a similar percentage of thrips flew regardless of age or type of cue present in the wind tunnel (average 44%, P>0.05).
AH-64 IHADSS aviator vision experiences in Operation Iraqi Freedom
NASA Astrophysics Data System (ADS)
Hiatt, Keith L.; Rash, Clarence E.; Harris, Eric S.; McGilberry, William H.
2004-09-01
Forty AH-64 Apache aviators representing a total of 8564 flight hours and 2260 combat hours during Operation Iraqi Freedom and its aftermath were surveyed for their visual experiences with the AH-64's monocular Integrated Helmet and Display Sighting System (IHADSS) helmet-mounted display in a combat environment. A major objective of this study was to determine if the frequencies of reports of visual complaints and illusions reported in the previous studies, addressing mostly benign training environments, differ in the more stressful combat environments. The most frequently reported visual complaints, both while and after flying, were visual discomfort and headache, which is consistent with previous studies. Frequencies of complaints after flying in the current study were numerically lower for all complaint types, but differences from previous studies are statistically significant only for visual discomfort and disorientation (vertigo). With the exception of "brownout/whiteout," reports of degraded visual cues in the current study were numerically lower for all types, but statistically significant only for impaired depth perception, decreased field of view, and inadvertent instrumental meteorological conditions. This study also found statistically lower reports of all static and dynamic illusions (with one exception, disorientation). This important finding is attributed to the generally flat and featureless geography present in a large portion of the Iraqi theater and to the shift in the way that the aviators use the two disparate visual inputs presented by the IHADSS monocular design (i.e., greater use of both eyes as opposed to concentrating primarily on display imagery).
Helicopter pilot estimation of self-altitude in a degraded visual environment
NASA Astrophysics Data System (ADS)
Crowley, John S.; Haworth, Loran A.; Szoboszlay, Zoltan P.; Lee, Alan G.
2000-06-01
The effect of night vision devices and degraded visual imagery on self-attitude perception is unknown. Thirteen Army aviators with normal vision flew five flights under various visual conditions in a modified AH-1 (Cobra) helicopter. Subjects estimated their altitude or flew to specified altitudes while flying a series of maneuvers. The results showed that subjects were better at detecting and controlling changes in altitude than they were at flying to or naming a specific altitude. In cruise flight and descent, the subjects tended to fly above the desired altitude, an error in the safe direction. While hovering, the direction of error was less predictable. In the low-level cruise flight scenario tested in this study, altitude perception was affected more by changes in image resolution than by changes in FOV or ocularity.
Visual flight control in naturalistic and artificial environments.
Baird, Emily; Dacke, Marie
2012-12-01
Although the visual flight control strategies of flying insects have evolved to cope with the complexity of the natural world, studies investigating this behaviour have typically been performed indoors using simplified two-dimensional artificial visual stimuli. How well do the results from these studies reflect the natural behaviour of flying insects considering the radical differences in contrast, spatial composition, colour and dimensionality between these visual environments? Here, we aim to answer this question by investigating the effect of three- and two-dimensional naturalistic and artificial scenes on bumblebee flight control in an outdoor setting and compare the results with those of similar experiments performed in an indoor setting. In particular, we focus on investigating the effect of axial (front-to-back) visual motion cues on ground speed and centring behaviour. Our results suggest that, in general, ground speed control and centring behaviour in bumblebees is not affected by whether the visual scene is two- or three dimensional, naturalistic or artificial, or whether the experiment is conducted indoors or outdoors. The only effect that we observe between naturalistic and artificial scenes on flight control is that when the visual scene is three-dimensional and the visual information on the floor is minimised, bumblebees fly further from the midline of the tunnel. The findings presented here have implications not only for understanding the mechanisms of visual flight control in bumblebees, but also for the results of past and future investigations into visually guided flight control in other insects.
Fuller, Sawyer Buckminster; Straw, Andrew D.; Peek, Martin Y.; Murray, Richard M.; Dickinson, Michael H.
2014-01-01
Flies and other insects use vision to regulate their groundspeed in flight, enabling them to fly in varying wind conditions. Compared with mechanosensory modalities, however, vision requires a long processing delay (~100 ms) that might introduce instability if operated at high gain. Flies also sense air motion with their antennae, but how this is used in flight control is unknown. We manipulated the antennal function of fruit flies by ablating their aristae, forcing them to rely on vision alone to regulate groundspeed. Arista-ablated flies in flight exhibited significantly greater groundspeed variability than intact flies. We then subjected them to a series of controlled impulsive wind gusts delivered by an air piston and experimentally manipulated antennae and visual feedback. The results show that an antenna-mediated response alters wing motion to cause flies to accelerate in the same direction as the gust. This response opposes flying into a headwind, but flies regularly fly upwind. To resolve this discrepancy, we obtained a dynamic model of the fly’s velocity regulator by fitting parameters of candidate models to our experimental data. The model suggests that the groundspeed variability of arista-ablated flies is the result of unstable feedback oscillations caused by the delay and high gain of visual feedback. The antenna response drives active damping with a shorter delay (~20 ms) to stabilize this regulator, in exchange for increasing the effect of rapid wind disturbances. This provides insight into flies’ multimodal sensory feedback architecture and constitutes a previously unknown role for the antennae. PMID:24639532
Visual summation in night-flying sweat bees: a theoretical study.
Theobald, Jamie Carroll; Greiner, Birgit; Wcislo, William T; Warrant, Eric J
2006-07-01
Bees are predominantly diurnal; only a few groups fly at night. An evolutionary limitation that bees must overcome to inhabit dim environments is their eye type: bees possess apposition compound eyes, which are poorly suited to vision in dim light. Here, we theoretically examine how nocturnal bees Megalopta genalis fly at light levels usually reserved for insects bearing more sensitive superposition eyes. We find that neural summation should greatly increase M. genalis's visual reliability. Predicted spatial summation closely matches the morphology of laminal neurons believed to mediate such summation. Improved reliability costs acuity, but dark adapted bees already suffer optical blurring, and summation further degrades vision only slightly.
Abolishment of Spontaneous Flight Turns in Visually Responsive Drosophila.
Ferris, Bennett Drew; Green, Jonathan; Maimon, Gaby
2018-01-22
Animals react rapidly to external stimuli, such as an approaching predator, but in other circumstances, they seem to act spontaneously, without any obvious external trigger. How do the neural processes mediating the execution of reflexive and spontaneous actions differ? We studied this question in tethered, flying Drosophila. We found that silencing a large but genetically defined set of non-motor neurons virtually eliminates spontaneous flight turns while preserving the tethered flies' ability to perform two types of visually evoked turns, demonstrating that, at least in flies, these two modes of action are almost completely dissociable. Copyright © 2017 Elsevier Ltd. All rights reserved.
Modeling visual-based pitch, lift and speed control strategies in hoverflies
Vercher, Jean-Louis
2018-01-01
To avoid crashing onto the floor, a free falling fly needs to trigger its wingbeats quickly and control the orientation of its thrust accurately and swiftly to stabilize its pitch and hence its speed. Behavioural data have suggested that the vertical optic flow produced by the fall and crossing the visual field plays a key role in this anti-crash response. Free fall behavior analyses have also suggested that flying insect may not rely on graviception to stabilize their flight. Based on these two assumptions, we have developed a model which accounts for hoverflies´ position and pitch orientation recorded in 3D with a fast stereo camera during experimental free falls. Our dynamic model shows that optic flow-based control combined with closed-loop control of the pitch suffice to stabilize the flight properly. In addition, our model sheds a new light on the visual-based feedback control of fly´s pitch, lift and thrust. Since graviceptive cues are possibly not used by flying insects, the use of a vertical reference to control the pitch is discussed, based on the results obtained on a complete dynamic model of a virtual fly falling in a textured corridor. This model would provide a useful tool for understanding more clearly how insects may or not estimate their absolute attitude. PMID:29361632
Contributions of the 12 neuron classes in the fly lamina to motion vision
Tuthill, John C.; Nern, Aljoscha; Holtz, Stephen L.; Rubin, Gerald M.; Reiser, Michael B.
2013-01-01
SUMMARY Motion detection is a fundamental neural computation performed by many sensory systems. In the fly, local motion computation is thought to occur within the first two layers of the visual system, the lamina and medulla. We constructed specific genetic driver lines for each of the 12 neuron classes in the lamina. We then depolarized and hyperpolarized each neuron type, and quantified fly behavioral responses to a diverse set of motion stimuli. We found that only a small number of lamina output neurons are essential for motion detection, while most neurons serve to sculpt and enhance these feedforward pathways. Two classes of feedback neurons (C2 and C3), and lamina output neurons (L2 and L4), are required for normal detection of directional motion stimuli. Our results reveal a prominent role for feedback and lateral interactions in motion processing, and demonstrate that motion-dependent behaviors rely on contributions from nearly all lamina neuron classes. PMID:23849200
Contributions of the 12 neuron classes in the fly lamina to motion vision.
Tuthill, John C; Nern, Aljoscha; Holtz, Stephen L; Rubin, Gerald M; Reiser, Michael B
2013-07-10
Motion detection is a fundamental neural computation performed by many sensory systems. In the fly, local motion computation is thought to occur within the first two layers of the visual system, the lamina and medulla. We constructed specific genetic driver lines for each of the 12 neuron classes in the lamina. We then depolarized and hyperpolarized each neuron type and quantified fly behavioral responses to a diverse set of motion stimuli. We found that only a small number of lamina output neurons are essential for motion detection, while most neurons serve to sculpt and enhance these feedforward pathways. Two classes of feedback neurons (C2 and C3), and lamina output neurons (L2 and L4), are required for normal detection of directional motion stimuli. Our results reveal a prominent role for feedback and lateral interactions in motion processing and demonstrate that motion-dependent behaviors rely on contributions from nearly all lamina neuron classes. Copyright © 2013 Elsevier Inc. All rights reserved.
Animal behavior: fly flight moves forward.
Fox, Jessica L; Frye, Mark
2013-04-08
A new study has resolved the paradox of how flies maintain reflexive aversion to your approaching swatter, whilst tolerating similar visual signals during normal forward flight. Copyright © 2013 Elsevier Ltd. All rights reserved.
Altus II aircraft flying over southern California desert
NASA Technical Reports Server (NTRS)
1998-01-01
The San Gabriel range is visible as the the remotely piloted Altus II flies over Southern California's high desert. The Altus II was flown as a performance and propulsion testbed for future high-altitude science platform aircraft under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program at the Dryden Flight Research Center, Edwards, Calif. The rear-engined Altus II and its sister ship, the Altus I, were built by General Atomics/Aeronautical Systems, Inc., of San Diego, Calif. They are designed for high-altitude, long-duration scientific sampling missions, and are powered by turbocharged piston engines. The Altus I, built for the Naval Postgraduate School, reached over 43,500 feet with a single-stage turbocharger feeding its four-cylinder Rotax engine in 1997, while the Altus II, incorporating a two-stage turbocharger built by Thermo-Mechanical Systems, reached and sustained an altitudeof 55,000 feet for four hours in 1999. A pilot in a control station on the ground flies the craft by radio signals, using visual cues from a video camera in the nose of the Altus and information from the craft's air data system.
The BOEING 777 - concurrent engineering and digital pre-assembly
DOE Office of Scientific and Technical Information (OSTI.GOV)
Abarbanel, B.
The processes created on the 777 for checking designs were called {open_quotes}digital pre-assembly{close_quotes}. Using FlyThru(tm), a spin-off of a Boeing advanced computing research project, engineers were able to view up to 1500 models (15000 solids) in 3d traversing that data at high speed. FlyThru(tm) was rapidly deployed in 1991 to meet the needs of the 777 for large scale product visualization and verification. The digital pre-assembly process has bad fantastic results. The 777 has had far fewer assembly and systems problems compared to previous airplane programs. Today, FlyThru(tm) is installed on hundreds of workstations on almost every airplane program, andmore » is being used on Space Station, F22, AWACS, and other defense projects. It`s applications have gone far beyond just design review. In many ways, FlyThru is a Data Warehouse supported by advanced tools for analysis. It is today being integrated with Knowledge Based Engineering geometry generation tools.« less
Triphan, Tilman; Nern, Aljoscha; Roberts, Sonia F.; Korff, Wyatt; Naiman, Daniel Q.; Strauss, Roland
2016-01-01
Climbing over chasms larger than step size is vital to fruit flies, since foraging and mating are achieved while walking. Flies avoid futile climbing attempts by processing parallax-motion vision to estimate gap width. To identify neuronal substrates of climbing control, we screened a large collection of fly lines with temporarily inactivated neuronal populations in a novel high-throughput assay described here. The observed climbing phenotypes were classified; lines in each group are reported. Selected lines were further analysed by high-resolution video cinematography. One striking class of flies attempts to climb chasms of unsurmountable width; expression analysis guided us to C2 optic-lobe interneurons. Inactivation of C2 or the closely related C3 neurons with highly specific intersectional driver lines consistently reproduced hyperactive climbing whereas strong or weak artificial depolarization of C2/C3 neurons strongly or mildly decreased climbing frequency. Contrast-manipulation experiments support our conclusion that C2/C3 neurons are part of the distance-evaluation system. PMID:27255169
DOE Office of Scientific and Technical Information (OSTI.GOV)
Rizzi, Silvio; Hereld, Mark; Insley, Joseph
In this work we perform in-situ visualization of molecular dynamics simulations, which can help scientists to visualize simulation output on-the-fly, without incurring storage overheads. We present a case study to couple LAMMPS, the large-scale molecular dynamics simulation code with vl3, our parallel framework for large-scale visualization and analysis. Our motivation is to identify effective approaches for covisualization and exploration of large-scale atomistic simulations at interactive frame rates.We propose a system of coupled libraries and describe its architecture, with an implementation that runs on GPU-based clusters. We present the results of strong and weak scalability experiments, as well as future researchmore » avenues based on our results.« less
Dourlen, Pierre; Bertin, Benjamin; Chatelain, Gilles; Robin, Marion; Napoletano, Francesco; Roux, Michel J.; Mollereau, Bertrand
2012-01-01
Tight regulation of the visual response is essential for photoreceptor function and survival. Visual response dysregulation often leads to photoreceptor cell degeneration, but the causes of such cell death are not well understood. In this study, we investigated a fatty acid transport protein (fatp) null mutation that caused adult-onset and progressive photoreceptor cell death. Consistent with fatp having a role in the retina, we showed that fatp is expressed in adult photoreceptors and accessory cells and that its re-expression in photoreceptors rescued photoreceptor viability in fatp mutants. The visual response in young fatp-mutant flies was abnormal with elevated electroretinogram amplitudes associated with high levels of Rhodopsin-1 (Rh1). Reducing Rh1 levels in rh1 mutants or depriving flies of vitamin A rescued photoreceptor cell death in fatp mutant flies. Our results indicate that fatp promotes photoreceptor survival by regulating Rh1 abundance. PMID:22844251
ERIC Educational Resources Information Center
King, Kenneth
2004-01-01
Few things capture the spirit of spring like flying a kite. Watching a kite dance and sail across a cloud spotted sky is not only a visually appealing experience it also provides a foundation for studies in science and mathematics. Put simply, a kite is an airfoil surface that flies when the forces of lift and thrust are greater than the forces of…
A homogeneous field for light adaptation.
DOT National Transportation Integrated Search
1966-09-01
Visual judgments of size, distance, slant, etc. in the flying situation are often made under reduced cue conditions, especially during night flying. In the experimental study of spatial perception under these conditions, experiments often require lon...
Method and System for Air Traffic Rerouting for Airspace Constraint Resolution
NASA Technical Reports Server (NTRS)
Erzberger, Heinz (Inventor); Morando, Alexander R. (Inventor); Sheth, Kapil S. (Inventor); McNally, B. David (Inventor); Clymer, Alexis A. (Inventor); Shih, Fu-tai (Inventor)
2017-01-01
A dynamic constraint avoidance route system automatically analyzes routes of aircraft flying, or to be flown, in or near constraint regions and attempts to find more time and fuel efficient reroutes around current and predicted constraints. The dynamic constraint avoidance route system continuously analyzes all flight routes and provides reroute advisories that are dynamically updated in real time. The dynamic constraint avoidance route system includes a graphical user interface that allows users to visualize, evaluate, modify if necessary, and implement proposed reroutes.
All-digital radar architecture
NASA Astrophysics Data System (ADS)
Molchanov, Pavlo A.
2014-10-01
All digital radar architecture requires exclude mechanical scan system. The phase antenna array is necessarily large because the array elements must be co-located with very precise dimensions and will need high accuracy phase processing system for aggregate and distribute T/R modules data to/from antenna elements. Even phase array cannot provide wide field of view. New nature inspired all digital radar architecture proposed. The fly's eye consists of multiple angularly spaced sensors giving the fly simultaneously thee wide-area visual coverage it needs to detect and avoid the threats around him. Fly eye radar antenna array consist multiple directional antennas loose distributed along perimeter of ground vehicle or aircraft and coupled with receiving/transmitting front end modules connected by digital interface to central processor. Non-steering antenna array allows creating all-digital radar with extreme flexible architecture. Fly eye radar architecture provides wide possibility of digital modulation and different waveform generation. Simultaneous correlation and integration of thousands signals per second from each point of surveillance area allows not only detecting of low level signals ((low profile targets), but help to recognize and classify signals (targets) by using diversity signals, polarization modulation and intelligent processing. Proposed all digital radar architecture with distributed directional antenna array can provide a 3D space vector to the jammer by verification direction of arrival for signals sources and as result jam/spoof protection not only for radar systems, but for communication systems and any navigation constellation system, for both encrypted or unencrypted signals, for not limited number or close positioned jammers.
Effects of Chronic Hypergravity on the Dopaminergic Neuronal System in Drosophila Melanogaster
NASA Technical Reports Server (NTRS)
Pelos, Andrew; Hosamani, Ravikumar; Bhattacharya, Sharmila
2017-01-01
Upon atmospheric exitre-entry and during training, astronauts are subjected to temporary periods of hypergravity, which has been implicated in the activation of oxidative stress pathways contributing to mitochondrial dysfunction and neuronal degeneration. The pathogenesis of Parkinsons disease and other neurodegenerative disorders is associated with oxidative damage to neurons involved in dopamine systems of the brain. Our study aims to examine the effects of a hypergravitational developmental environment on the degeneration of dopaminergic systems in Drosophila melanogaster. Male and female flies (Gal4-UAS transgenic line) were hatched and raised to adulthood in centrifugal hypergravity (97rpm, 3g). The nuclear expression of the reporter, Green Fluorescent Protein (GFP) is driven by the dopaminergic enzyme tyrosine hydroxylase (TH) promoter, allowing for the targeted visualization of dopamine producing neurons. After being raised to adulthood and kept in hypergravity until 18 days of age, flies were dissected and the expression of TH was measured by fluorescence confocal microscopy. TH expression in the fly brains was used to obtain counts of healthy dopaminergic neurons for flies raised in chronic hypergravity and control groups. Dopaminergic neuron expression data were compared with those of previous studies that limited hypergravity exposure to late life in order to determine the flies adaptability to the gravitational environment when raised from hatching through adulthood. Overall, we observed a significant effect of chronic hypergravity exposure contributing to deficits in dopaminergic neuron expression (p 0.003). Flies raised in 3g had on average lower dopaminergic neuron counts (mean 97.7) when compared with flies raised in 1g (mean 122.8). We suspect these lower levels of TH expression are a result of oxidative dopaminergic cell loss in flies raised in hypergravity. In future studies, we hope to further elucidate the mechanism by which hypergravity-induced oxidative stress damages the dopaminergic neuronal system, as well as examining possible chemical countermeasures to the hypergravity-induced oxidative stress response in dopaminergic neurons in order to combat cell death and consequent mental and behavioral deficits.
Chronic Hypergravity Induces Changes in the Dopaminergic Neuronal System in Drosophila Melanogaster
NASA Technical Reports Server (NTRS)
Pelos, Andrew; Hosamani, Ravikumar; Bhattacharya, Sharmila
2017-01-01
Upon atmospheric exitre-entry and during training, astronauts are subjected to temporary periods of hypergravity, which has been implicated in the activation of oxidative stress pathways contributing to mitochondrial dysfunction and neuronal degeneration. The pathogenesis of Parkinsons disease and other neurodegenerative disorders is associated with oxidative damage to neurons involved in dopamine systems of the brain. Our study aims to examine the effects of a hypergravitational developmental environment on the degeneration of dopaminergic systems in Drosophila melanogaster. Male and female flies (Gal4-UAS transgenic line) were hatched and raised to adulthood in centrifugal hypergravity (97rpm, 3g). The nuclear expression of the reporter, Green Fluorescent Protein (GFP) is driven by the dopaminergic enzyme tyrosine hydroxylase (TH) promoter, allowing for the targeted visualization of dopamine producing neurons. After being raised to adulthood and kept in hypergravity until 18 days of age, flies were dissected and the expression of TH was measured by fluorescence confocal microscopy. TH expression in the fly brains was used to obtain counts of healthy dopaminergic neurons for flies raised in chronic hypergravity and control groups. Dopaminergic neuron expression data were compared with those of previous studies that limited hypergravity exposure to late life in order to determine the flies adaptability to the gravitational environment when raised from hatching through adulthood. Overall, we observed a significant effect of chronic hypergravity exposure contributing to deficits in dopaminergic neuron expression (p 0.003). Flies raised in 3g had on average lower dopaminergic neuron counts (mean 97.7) when compared with flies raised in 1g (mean 122.8). We suspect these lower levels of TH expression are a result of oxidative dopaminergic cell loss in flies raised in hypergravity. In future studies, we hope to further elucidate the mechanism by which hypergravity-induced oxidative stress damages the dopaminergic neuronal system, as well as examining possible chemical countermeasures to the hypergravity-induced oxidative stress response in dopaminergic neurons in order to combat cell death and consequent mental and behavioral deficits.
NASA Technical Reports Server (NTRS)
Pirrello, C. J.; Hardin, R. D.; Capelluro, L. P.; Harrison, W. D.
1971-01-01
The general purpose capabilities of government and industry in the area of real time engineering flight simulation are discussed. The information covers computer equipment, visual systems, crew stations, and motion systems, along with brief statements of facility capabilities. Facility construction and typical operational costs are included where available. The facilities provide for economical and safe solutions to vehicle design, performance, control, and flying qualities problems of manned and unmanned flight systems.
Blahó, Miklós; Egri, Adám; Száz, Dénes; Kriska, György; Akesson, Susanne; Horváth, Gábor
2013-07-02
As with mosquitoes, female tabanid flies search for mammalian hosts by visual and olfactory cues, because they require a blood meal before being able to produce and lay eggs. Polarotactic tabanid flies find striped or spotted patterns with intensity and/or polarisation modulation visually less attractive than homogeneous white, brown or black targets. Thus, this reduced optical attractiveness to tabanids can be one of the functions of striped or spotty coat patterns in ungulates. Ungulates emit CO2 via their breath, while ammonia originates from their decaying urine. As host-seeking female tabanids are strongly attracted to CO2 and ammonia, the question arises whether the poor visual attractiveness of stripes and spots to tabanids is or is not overcome by olfactory attractiveness. To answer this question we performed two field experiments in which the attractiveness to tabanid flies of homogeneous white, black and black-and-white striped three-dimensional targets (spheres and cylinders) and horse models provided with CO2 and ammonia was studied. Since tabanids are positively polarotactic, i.e. attracted to strongly and linearly polarised light, we measured the reflection-polarisation patterns of the test surfaces and demonstrated that these patterns were practically the same as those of real horses and zebras. We show here that striped targets are significantly less attractive to host-seeking female tabanids than homogeneous white or black targets, even when they emit tabanid-luring CO2 and ammonia. Although CO2 and ammonia increased the number of attracted tabanids, these chemicals did not overcome the weak visual attractiveness of stripes to host-seeking female tabanids. This result demonstrates the visual protection of striped coat patterns against attacks from blood-sucking dipterans, such as horseflies, known to transmit lethal diseases to ungulates. © 2013.
Visual Attention in Flies-Dopamine in the Mushroom Bodies Mediates the After-Effect of Cueing.
Koenig, Sebastian; Wolf, Reinhard; Heisenberg, Martin
2016-01-01
Visual environments may simultaneously comprise stimuli of different significance. Often such stimuli require incompatible responses. Selective visual attention allows an animal to respond exclusively to the stimuli at a certain location in the visual field. In the process of establishing its focus of attention the animal can be influenced by external cues. Here we characterize the behavioral properties and neural mechanism of cueing in the fly Drosophila melanogaster. A cue can be attractive, repulsive or ineffective depending upon (e.g.) its visual properties and location in the visual field. Dopamine signaling in the brain is required to maintain the effect of cueing once the cue has disappeared. Raising or lowering dopamine at the synapse abolishes this after-effect. Specifically, dopamine is necessary and sufficient in the αβ-lobes of the mushroom bodies. Evidence is provided for an involvement of the αβposterior Kenyon cells.
ERIC Educational Resources Information Center
Ricard, G. L.; And Others
The cooperative Navy/Air Force project described is aimed at the problem of image-flutter encountered when visual displays that present computer generated images are used for the simulation of certain flying situations. Two experiments are described which extend laboratory work on delay compensation schemes to the simulation of formation flight in…
Detection of Blood in Stable Flies (Diptera: Muscidae) with Hemoccult® Test Strips
USDA-ARS?s Scientific Manuscript database
Hemoccult® test kits were used to detect the remnants of blood meals in stable flies. The strips were able to detect remnants of blood meals in > 90% of the stable flies up to 8 days after blood feeding. This can be compared with detecting blood in the gut visually which was possible in less than 5%...
A visual horizon affects steering responses during flight in fruit flies.
Caballero, Jorge; Mazo, Chantell; Rodriguez-Pinto, Ivan; Theobald, Jamie C
2015-09-01
To navigate well through three-dimensional environments, animals must in some way gauge the distances to objects and features around them. Humans use a variety of visual cues to do this, but insects, with their small size and rigid eyes, are constrained to a more limited range of possible depth cues. For example, insects attend to relative image motion when they move, but cannot change the optical power of their eyes to estimate distance. On clear days, the horizon is one of the most salient visual features in nature, offering clues about orientation, altitude and, for humans, distance to objects. We set out to determine whether flying fruit flies treat moving features as farther off when they are near the horizon. Tethered flies respond strongly to moving images they perceive as close. We measured the strength of steering responses while independently varying the elevation of moving stimuli and the elevation of a virtual horizon. We found responses to vertical bars are increased by negative elevations of their bases relative to the horizon, closely correlated with the inverse of apparent distance. In other words, a bar that dips far below the horizon elicits a strong response, consistent with using the horizon as a depth cue. Wide-field motion also had an enhanced effect below the horizon, but this was only prevalent when flies were additionally motivated with hunger. These responses may help flies tune behaviors to nearby objects and features when they are too far off for motion parallax. © 2015. Published by The Company of Biologists Ltd.
Ngo, Kathy T.; Andrade, Ingrid; Hartenstein, Volker
2018-01-01
Visual information processing in animals with large image forming eyes is carried out in highly structured retinotopically ordered neuropils. Visual neuropils in Drosophila form the optic lobe, which consists of four serially arranged major subdivisions; the lamina, medulla, lobula and lobula plate; the latter three of these are further subdivided into multiple layers. The visual neuropils are formed by more than 100 different cell types, distributed and interconnected in an invariant highly regular pattern. This pattern relies on a protracted sequence of developmental steps, whereby different cell types are born at specific time points and nerve connections are formed in a tightly controlled sequence that has to be coordinated among the different visual neuropils. The developing fly visual system has become a highly regarded and widely studied paradigm to investigate the genetic mechanisms that control the formation of neural circuits. However, these studies are often made difficult by the complex and shifting patterns in which different types of neurons and their connections are distributed throughout development. In the present paper we have reconstructed the three-dimensional architecture of the Drosophila optic lobe from the early larva to the adult. Based on specific markers, we were able to distinguish the populations of progenitors of the four optic neuropils and map the neurons and their connections. Our paper presents sets of annotated confocal z-projections and animated 3D digital models of these structures for representative stages. The data reveal the temporally coordinated growth of the optic neuropils, and clarify how the position and orientation of the neuropils and interconnecting tracts (inner and outer optic chiasm) changes over time. Finally, we have analyzed the emergence of the discrete layers of the medulla and lobula complex using the same markers (DN-cadherin, Brp) employed to systematically explore the structure and development of the central brain neuropil. Our work will facilitate experimental studies of the molecular mechanisms regulating neuronal fate and connectivity in the fly visual system, which bears many fundamental similarities with the retina of vertebrates. PMID:28533086
SVGMap: configurable image browser for experimental data.
Rafael-Palou, Xavier; Schroeder, Michael P; Lopez-Bigas, Nuria
2012-01-01
Spatial data visualization is very useful to represent biological data and quickly interpret the results. For instance, to show the expression pattern of a gene in different tissues of a fly, an intuitive approach is to draw the fly with the corresponding tissues and color the expression of the gene in each of them. However, the creation of these visual representations may be a burdensome task. Here we present SVGMap, a java application that automatizes the generation of high-quality graphics for singular data items (e.g. genes) and biological conditions. SVGMap contains a browser that allows the user to navigate the different images created and can be used as a web-based results publishing tool. SVGMap is freely available as precompiled java package as well as source code at http://bg.upf.edu/svgmap. It requires Java 6 and any recent web browser with JavaScript enabled. The software can be run on Linux, Mac OS X and Windows systems. nuria.lopez@upf.edu
Dowse, H B; Dushay, M S; Hall, J C; Ringo, J M
1989-07-01
Free-running locomotor activity and eclosion rhythms of Drosophila melanogaster, mutant at the disconnected (disco) locus, are substantially different from the wild-type phenotype. Initial periodogram analysis revealed little or no rhythmicity (Dushay et al., 1989). We have reanalyzed the locomotor activity data using high-resolution signal analysis (maximum-entropy spectral analysis, or MESA). These analyses, corroborated by autocorrelograms, uncovered significant residual circadian rhythmicity and strong ultradian rhythms in most of the animals tested. In this regard the disco mutants are much like flies expressing mutant alleles of the period gene, as well as wild-type flies reared throughout life in constant darkness. We hypothesize that light normally triggers the coupling of multiple ultradian oscillators into a functional circadian clock and that this process is disrupted in disco flies as a result of the neural lesion.
Dynamics of memory-guided choice behavior in Drosophila
ICHINOSE, Toshiharu; TANIMOTO, Hiromu
2016-01-01
Memory retrieval requires both accuracy and speed. Olfactory learning of the fruit fly Drosophila melanogaster serves as a powerful model system to identify molecular and neuronal substrates of memory and memory-guided behavior. The behavioral expression of olfactory memory has traditionally been tested as a conditioned odor response in a simple T-maze, which measures the result, but not the speed, of odor choice. Here, we developed multiplexed T-mazes that allow video recording of the choice behavior. Automatic fly counting in each arm of the maze visualizes choice dynamics. Using this setup, we show that the transient blockade of serotonergic neurons slows down the choice, while leaving the eventual choice intact. In contrast, activation of the same neurons impairs the eventual performance leaving the choice speed unchanged. Our new apparatus contributes to elucidating how the speed and the accuracy of memory retrieval are implemented in the fly brain. PMID:27725473
UTM TCL2 Software Requirements
NASA Technical Reports Server (NTRS)
Smith, Irene S.; Rios, Joseph L.; McGuirk, Patrick O.; Mulfinger, Daniel G.; Venkatesan, Priya; Smith, David R.; Baskaran, Vijayakumar; Wang, Leo
2017-01-01
The Unmanned Aircraft Systems (UAS) Traffic Management (UTM) Technical Capability Level (TCL) 2 software implements the UTM TCL 2 software requirements described herein. These software requirements are linked to the higher level UTM TCL 2 System Requirements. Each successive TCL implements additional UTM functionality, enabling additional use cases. TCL 2 demonstrated how to enable expanded multiple operations by implementing automation for beyond visual line-of-sight, tracking operations, and operations flying over sparsely populated areas.
2011-03-09
anu.edu.au Nocturnal visual orientation in flying insects: a benchmark for the design of vision-based sensors in Micro-Aerial Vehicles Report...9 10 Technical horizon sensors Over the past few years, a remarkable proliferation of designs for micro-aerial vehicles (MAVs) has occurred...possible elevations, it may severely degrade the performance of sensors by local saturation. Therefore it is necessary to find a method whereby the effect
Adaptation without parameter change: Dynamic gain control in motion detection
Borst, Alexander; Flanagin, Virginia L.; Sompolinsky, Haim
2005-01-01
Many sensory systems adapt their input-output relationship to changes in the statistics of the ambient stimulus. Such adaptive behavior has been measured in a motion detection sensitive neuron of the fly visual system, H1. The rapid adaptation of the velocity response gain has been interpreted as evidence of optimal matching of the H1 response to the dynamic range of the stimulus, thereby maximizing its information transmission. Here, we show that correlation-type motion detectors, which are commonly thought to underlie fly motion vision, intrinsically possess adaptive properties. Increasing the amplitude of the velocity fluctuations leads to a decrease of the effective gain and the time constant of the velocity response without any change in the parameters of these detectors. The seemingly complex property of this adaptation turns out to be a straightforward consequence of the multidimensionality of the stimulus and the nonlinear nature of the system. PMID:15833815
Experiments in teleoperator and autonomous control of space robotic vehicles
NASA Technical Reports Server (NTRS)
Alexander, Harold L.
1991-01-01
A program of research embracing teleoperator and automatic navigational control of freely flying satellite robots is presented. Current research goals include: (1) developing visual operator interfaces for improved vehicle teleoperation; (2) determining the effects of different visual interface system designs on operator performance; and (3) achieving autonomous vision-based vehicle navigation and control. This research program combines virtual-environment teleoperation studies and neutral-buoyancy experiments using a space-robot simulator vehicle currently under development. Visual-interface design options under investigation include monoscopic versus stereoscopic displays and cameras, helmet-mounted versus panel-mounted display monitors, head-tracking versus fixed or manually steerable remote cameras, and the provision of vehicle-fixed visual cues, or markers, in the remote scene for improved sensing of vehicle position, orientation, and motion.
Multisensory Control of Stabilization Reflexes
2012-08-22
Dr Simon Schultz (Neural Coding), Dr Manos Drakakis (Low-power VLSI technology), and Dr Reiko Tanaka (Compound Control). To study the functional...Krapp H.G., and Schultz S.R.: Spike-triggered independent component analysis: application to a fly motion-sensitive neuron. Visual Neuroscience, 8...Tanaka, RI.: Characterization of insect gaze control systems. 18th World Congress of International Federation of Automated Control (IFAC), Milan
Altus II aircraft flying over southern California desert
NASA Technical Reports Server (NTRS)
1998-01-01
The snow-capped peak of Mt. San Antonio in the San Gabriel range is visible as the the remotely piloted Altus II flies over Southern California's high desert. The Altus II was flown as a performance and propulsion testbed for future high-altitude science platform aircraft under NASA's Environmental Research Aircraft and Sensor Technology (ERAST) program at the Dryden Flight Research Center, Edwards, Calif. The rear-engined Altus II and its sister ship, the Altus I, were built by General Atomics/Aeronautical Systems, Inc., of San Diego, Calif. They are designed for high-altitude, long-duration scientific sampling missions, and are powered by turbocharged piston engines. The Altus I, built for the Naval Postgraduate School, reached over 43,500 feet with a single-stage turbocharger feeding its four-cylinder Rotax engine in 1997, while the Altus II, incorporating a two-stage turbocharger built by Thermo-Mechanical Systems, reached and sustained an altitudeof 55,000 feet for four hours in 1999. A pilot in a control station on the ground flies the craft by radio signals, using visual cues from a video camera in the nose of the Altus and information from the craft's air data system.
Development of an advanced pitch active control system for a wide body jet aircraft
NASA Technical Reports Server (NTRS)
Guinn, Wiley A.; Rising, Jerry J.; Davis, Walt J.
1984-01-01
An advanced PACS control law was developed for a commercial wide-body transport (Lockheed L-1011) by using modern control theory. Validity of the control law was demonstrated by piloted flight simulation tests on the NASA Langley visual motion simulator. The PACS design objective was to develop a PACS that would provide good flying qualities to negative 10 percent static stability margins that were equivalent to those of the baseline aircraft at a 15 percent static stability margin which is normal for the L-1011. Also, the PACS was to compensate for high-Mach/high-g instabilities that degrade flying qualities during upset recoveries and maneuvers. The piloted flight simulation tests showed that the PACS met the design objectives. The simulation demonstrated good flying qualities to negative 20 percent static stability margins for hold, cruise and high-speed flight conditions. Analysis and wind tunnel tests performed on other Lockheed programs indicate that the PACS could be used on an advanced transport configuration to provide a 4 percent fuel savings which results from reduced trim drag by flying at negative static stability margins.
Sensory Coordination of Insect Flight
2009-12-29
begun to study how fruit flies pinpoint the location of an odor source ( banana mash placed within a black pole, a strong visual landmark against a...hover feeding, flower tracking, odor tracking etc. Figure 4: Extracting wing and body kinematics from freely flying Drosophila melanogaster. (A
Surprising characteristics of visual systems of invertebrates.
González-Martín-Moro, J; Hernández-Verdejo, J L; Jiménez-Gahete, A E
2017-01-01
To communicate relevant and striking aspects about the visual system of some close invertebrates. Review of the related literature. The capacity of snails to regenerate a complete eye, the benefit of the oval shape of the compound eye of many flying insects as a way of stabilising the image during flight, the potential advantages related to the extreme refractive error that characterises the ocelli of many insects, as well as the ability to detect polarised light as a navigation system, are some of the surprising capabilities present in the small invertebrate eyes that are described in this work. The invertebrate eyes have capabilities and sensorial modalities that are not present in the human eye. The study of the eyes of these animals can help us to improve our understanding of our visual system, and inspire the development of optical devices. Copyright © 2016 Sociedad Española de Oftalmología. Publicado por Elsevier España, S.L.U. All rights reserved.
1983-11-04
visual acuity in amblyopia , using steady-state visual evoked potentials. In J. E. Desmedt (Ed.), Visual evoked potentials in man: new developments... amblyopia by the evoked potential method. Ophthalmologica, 1977s 175, 159-164. 61. Regan, D. & Spekreijse, H. Auditory-visual interactions and the
NASA Technical Reports Server (NTRS)
McNally, B. David (Inventor); Erzberger, Heinz (Inventor); Sheth, Kapil (Inventor)
2015-01-01
A dynamic weather route system automatically analyzes routes for in-flight aircraft flying in convective weather regions and attempts to find more time and fuel efficient reroutes around current and predicted weather cells. The dynamic weather route system continuously analyzes all flights and provides reroute advisories that are dynamically updated in real time while the aircraft are in flight. The dynamic weather route system includes a graphical user interface that allows users to visualize, evaluate, modify if necessary, and implement proposed reroutes.
TRAINING - GEMINI-TITAN (GT)-5 - TX
1965-06-18
S65-35563 (18 June 1965) --- Astronauts L. Gordon Cooper Jr. (left), command pilot; and Charles Conrad Jr., pilot, the prime crew of the Gemini-5 spaceflight, prepare their cameras while aboard a C-130 aircraft flying near Laredo, Texas. The two astronauts are taking part in a series of visual acuity experiments to aid them in learning to identify known terrestrial features under controlled conditions. Knowledge gained from these experiments will have later application for space pilots identifying terrestrial features from space. Dr. John Billingham, chief, Environmental Physiology Branch, Crew Systems Division, is in charge of the Visual Acuity Experiments.
A bio-inspired flying robot sheds light on insect piloting abilities.
Franceschini, Nicolas; Ruffier, Franck; Serres, Julien
2007-02-20
When insects are flying forward, the image of the ground sweeps backward across their ventral viewfield and forms an "optic flow," which depends on both the groundspeed and the groundheight. To explain how these animals manage to avoid the ground by using this visual motion cue, we suggest that insect navigation hinges on a visual-feedback loop we have called the optic-flow regulator, which controls the vertical lift. To test this idea, we built a micro-helicopter equipped with an optic-flow regulator and a bio-inspired optic-flow sensor. This fly-by-sight micro-robot can perform exacting tasks such as take-off, level flight, and landing. Our control scheme accounts for many hitherto unexplained findings published during the last 70 years on insects' visually guided performances; for example, it accounts for the fact that honeybees descend in a headwind, land with a constant slope, and drown when travelling over mirror-smooth water. Our control scheme explains how insects manage to fly safely without any of the instruments used onboard aircraft to measure the groundheight, groundspeed, and descent speed. An optic-flow regulator is quite simple in terms of its neural implementation and just as appropriate for insects as it would be for aircraft.
The role of experience in flight behaviour of Drosophila.
Hesselberg, Thomas; Lehmann, Fritz-Olaf
2009-10-01
Experience plays a key role in the acquisition of complex motor skills in running and flight of many vertebrates. To evaluate the significance of previous experience for the efficiency of motor behaviour in an insect, we investigated the flight behaviour of the fruit fly Drosophila. We reared flies in chambers in which the animals could freely walk and extend their wings, but could not gain any flight experience. These naive animals were compared with control flies under both open- and closed-loop tethered flight conditions in a flight simulator as well as in a free-flight arena. The data suggest that the overall flight behaviour in Drosophila seems to be predetermined because both groups exhibited similar mean stroke amplitude and stroke frequency, similar open-loop responses to visual stimulation and the immediate ability to track visual objects under closed-loop feedback conditions. In short free flight bouts, peak saccadic turning rate, angular acceleration, peak horizontal speed and flight altitude were also similar in naive and control flies. However, we found significant changes in other key parameters in naive animals such as a reduction in mean horizontal speed (-23%) and subtle changes in mean turning rate (-48%). Naive flies produced 25% less yaw torque-equivalent stroke amplitudes than the controls in response to a visual stripe rotating in open loop around the tethered animal, potentially suggesting a flight-dependent adaptation of the visuo-motor gain in the control group. This change ceased after the animals experienced visual closed-loop feedback. During closed-loop flight conditions, naive flies had 53% larger differences in left and right stroke amplitude when fixating a visual object, thus steering control was less precise. We discuss two alternative hypotheses to explain our results: the ;neuronal experience' hypothesis, suggesting that there are some elements of learning and fine-tuning involved during the first flight experiences in Drosophila and the ;muscular exercise' hypothesis. Our experiments support the first hypothesis because maximum locomotor capacity seems not to be significantly impaired in the naive group. Although this study primarily confirms the genetic pre-disposition for flight in Drosophila, previous experience may apparently adjust locomotor fine control and aerial performance, although this effect seems to be small compared with vertebrates.
Solar-powered electrocuting trap for controlling house flies and stable flies (Diptera: Muscidae).
Pickens, L G; Mills, G D
1993-09-01
A portable trap was constructed that was visually attractive to house flies, Musca domestica L., and stable flies, Stomoxys calcitrans (L.), outdoors. The trap was made of a white and yellow pyramid placed on top of a white vertical base that had large cutouts in each side. Attracted flies were killed by means of solar-powered electrocuting grids. Three traps killed an average of 1,360 house flies and 1,190 stable flies per day at a manure dump and were effective in attracting flies under both cool (< 23 degrees C) and warm (> 30 degrees C) temperatures. Both species of flies were most attracted to the eastern side of the trap, but house flies preferred yellow in cool mornings and white in warm afternoons. When air temperatures were > 30 degrees C, both house flies and stable flies went into the shaded base of the trap or into tunnels. Most house flies were killed on the pyramidal top of the trap, whereas most stable flies were killed on the vertical base. Opaque fiberglass tunnels with central electrocuting grids were simpler and cheaper, although less effective, for stable flies.
Morrison, William R; Lee, Doo-Hyung; Reissig, W Harvey; Combs, David; Leahy, Kathleen; Tuttle, Arthur; Cooley, Daniel; Leskey, Tracy C
2016-08-01
Investigating the chemical ecology of agricultural systems continues to be a salient part of integrated pest management programs. Apple maggot fly, a key pest of apple in eastern North America, is a visual specialist with attraction to host fruit-mimicking cues. These cues have been incorporated into red spherical traps used for both monitoring and behaviorally based management. Incorporating generalist or specialist olfactory cues can potentially increase the overall success of this management system. The primary aim of this study was to evaluate the attractiveness of a generalist olfactory cue, ammonium carbonate, and the specialist olfactory cue, a five-component apple volatile blend, when included as a component of a red attracticidal sphere system. Secondly, we assessed how critical it was to maintain minimal deviation from the optimal, full-round specialist visual stimulus provided by red spheres. Finally, attracticidal spheres were deployed with specialist olfactory cues in commercial apple orchards to evaluate their potential for effective management of apple maggot. Ammonium carbonate did not increase residency, feeding time, or mortality in the laboratory-based trials. Field deployment of specialist olfactory cues increased apple maggot captures on red spheres, while the generalist cue did not. Apple maggot tolerated some deviation from the optimal visual stimulus without reducing captures on red spheres. Attracticidal spheres hung in perimeter trees in orchards resulted in acceptable and statistically identical levels of control compared with standard insecticide programs used by growers. Overall, our study contributes valuable information for developing a reliable attract-and-kill system for apple maggot. Published by Oxford University Press on behalf of Entomological Society of America 2016. This work is written by US Government employees and is in the public domain in the United States.
Visual control of navigation in insects and its relevance for robotics.
Srinivasan, Mandyam V
2011-08-01
Flying insects display remarkable agility, despite their diminutive eyes and brains. This review describes our growing understanding of how these creatures use visual information to stabilize flight, avoid collisions with objects, regulate flight speed, detect and intercept other flying insects such as mates or prey, navigate to a distant food source, and orchestrate flawless landings. It also outlines the ways in which these insights are now being used to develop novel, biologically inspired strategies for the guidance of autonomous, airborne vehicles. Copyright © 2011 Elsevier Ltd. All rights reserved.
Green, T A; Prokopy, R J; Hosmer, D W
1994-09-01
Mature female apple maggot flies,Rhagoletis pomonella (Walsh), were released individually onto a single potted, fruitless hawthorne tree in the center of an open field. The tree was surrounded by four 1-m(2) plywood host tree models painted green or white, with or without synthetic host fruit odor (butyl hexanoate), and placed at one of several distances from the release tree. Each fly was permitted to forage freely on the release tree for up to 1 hr, or until it left the tree. Flies left the tree significantly sooner when green models with host fruit were present at 0.5, 1.5, or 2.5 m distance from the release tree than when these models were placed at a greater distance (4.5 m) from the release tree or when no models were present. Flies responded detectably to 1-m(2) models without odor up to a maximum distance of 1.5 m. These results suggest that female apple maggot flies did not detect green 1-m(2) models with odor 4.5 m away or models without odor 2.5 m or more away. Flies responded to white models with and without odor to a much lesser extent, both in terms of response distance and flight to and alightment upon models. Increasing model size to 2 m(2) increased the distance to 2.5 m at which flies responded to green models without odor. Decreasing model size to 0.5 m(2) reduced fly responsiveness to green or white models. The presence of host fruit odor alone, without the visual stimulus of a green model, did not influence residence time on the release tree.
Walking modulates speed sensitivity in Drosophila motion vision.
Chiappe, M Eugenia; Seelig, Johannes D; Reiser, Michael B; Jayaraman, Vivek
2010-08-24
Changes in behavioral state modify neural activity in many systems. In some vertebrates such modulation has been observed and interpreted in the context of attention and sensorimotor coordinate transformations. Here we report state-dependent activity modulations during walking in a visual-motor pathway of Drosophila. We used two-photon imaging to monitor intracellular calcium activity in motion-sensitive lobula plate tangential cells (LPTCs) in head-fixed Drosophila walking on an air-supported ball. Cells of the horizontal system (HS)--a subgroup of LPTCs--showed stronger calcium transients in response to visual motion when flies were walking rather than resting. The amplified responses were also correlated with walking speed. Moreover, HS neurons showed a relatively higher gain in response strength at higher temporal frequencies, and their optimum temporal frequency was shifted toward higher motion speeds. Walking-dependent modulation of HS neurons in the Drosophila visual system may constitute a mechanism to facilitate processing of higher image speeds in behavioral contexts where these speeds of visual motion are relevant for course stabilization. Copyright 2010 Elsevier Ltd. All rights reserved.
TopicLens: Efficient Multi-Level Visual Topic Exploration of Large-Scale Document Collections.
Kim, Minjeong; Kang, Kyeongpil; Park, Deokgun; Choo, Jaegul; Elmqvist, Niklas
2017-01-01
Topic modeling, which reveals underlying topics of a document corpus, has been actively adopted in visual analytics for large-scale document collections. However, due to its significant processing time and non-interactive nature, topic modeling has so far not been tightly integrated into a visual analytics workflow. Instead, most such systems are limited to utilizing a fixed, initial set of topics. Motivated by this gap in the literature, we propose a novel interaction technique called TopicLens that allows a user to dynamically explore data through a lens interface where topic modeling and the corresponding 2D embedding are efficiently computed on the fly. To support this interaction in real time while maintaining view consistency, we propose a novel efficient topic modeling method and a semi-supervised 2D embedding algorithm. Our work is based on improving state-of-the-art methods such as nonnegative matrix factorization and t-distributed stochastic neighbor embedding. Furthermore, we have built a web-based visual analytics system integrated with TopicLens. We use this system to measure the performance and the visualization quality of our proposed methods. We provide several scenarios showcasing the capability of TopicLens using real-world datasets.
Trapping related to phytosanitary status and trade
USDA-ARS?s Scientific Manuscript database
Detection of incipient fruit fly populations can occur through a number of means such as visual surveys, fruit cutting (to reveal the presence of immatures), collection and holding of fruits to determine if fruit flies emerge from the collected fruits and perhaps the most commonly used method, trapp...
Effectiveness of basic display augmentation in vehicular control by visual field cues
NASA Technical Reports Server (NTRS)
Grunwald, A. J.; Merhav, S. J.
1978-01-01
The paper investigates the effectiveness of different basic display augmentation concepts - fixed reticle, velocity vector, and predicted future vehicle path - for RPVs controlled by a vehicle-mounted TV camera. The task is lateral manual control of a low flying RPV along a straight reference line in the presence of random side gusts. The man-machine system and the visual interface are modeled as a linear time-invariant system. Minimization of a quadratic performance criterion is assumed to underlie the control strategy of a well-trained human operator. The solution for the optimal feedback matrix enables the explicit computation of the variances of lateral deviation and directional error of the vehicle and of the control force that are used as performance measures.
Aminergic neuromodulation of associative visual learning in harnessed honey bees.
Mancini, Nino; Giurfa, Martin; Sandoz, Jean-Christophe; Avarguès-Weber, Aurore
2018-05-21
The honey bee Apis mellifera is a major insect model for studying visual cognition. Free-flying honey bees learn to associate different visual cues with a sucrose reward and may deploy sophisticated cognitive strategies to this end. Yet, the neural bases of these capacities cannot be studied in flying insects. Conversely, immobilized bees are accessible to neurobiological investigation but training them to respond appetitively to visual stimuli paired with sucrose reward is difficult. Here we succeeded in coupling visual conditioning in harnessed bees with pharmacological analyses on the role of octopamine (OA), dopamine (DA) and serotonin (5-HT) in visual learning. We also studied if and how these biogenic amines modulate sucrose responsiveness and phototaxis behaviour as intact reward and visual perception are essential prerequisites for appetitive visual learning. Our results suggest that both octopaminergic and dopaminergic signaling mediate either the appetitive sucrose signaling or the association between color and sucrose reward in the bee brain. Enhancing and inhibiting serotonergic signaling both compromised learning performances, probably via an impairment of visual perception. We thus provide a first analysis of the role of aminergic signaling in visual learning and retention in the honey bee and discuss further research trends necessary to understand the neural bases of visual cognition in this insect. Copyright © 2018 Elsevier Inc. All rights reserved.
To crash or not to crash: how do hoverflies cope with free-fall situations and weightlessness?
Goulard, Roman; Vercher, Jean-Louis; Viollet, Stéphane
2016-08-15
Insects' aptitude to perform hovering, automatic landing and tracking tasks involves accurately controlling their head and body roll and pitch movements, but how this attitude control depends on an internal estimation of gravity orientation is still an open question. Gravity perception in flying insects has mainly been studied in terms of grounded animals' tactile orientation responses, but it has not yet been established whether hoverflies use gravity perception cues to detect a nearly weightless state at an early stage. Ground-based microgravity simulators provide biologists with useful tools for studying the effects of changes in gravity. However, in view of the cost and the complexity of these set-ups, an alternative Earth-based free-fall procedure was developed with which flying insects can be briefly exposed to microgravity under various visual conditions. Hoverflies frequently initiated wingbeats in response to an imposed free fall in all the conditions tested, but managed to avoid crashing only in variably structured visual environments, and only episodically in darkness. Our results reveal that the crash-avoidance performance of these insects in various visual environments suggests the existence of a multisensory control system based mainly on vision rather than gravity perception. © 2016. Published by The Company of Biologists Ltd.
White, Thomas E; Kemp, Darrell J
2017-08-14
Theory predicts that colour polymorphism may be favored by variation in the visual context under which signals are perceived. The context encompasses all environmental determinants of light availability and propagation, but also the dynamics of perception in receivers. Color vision involves the neural separation of information into spectral versus luminance channels, which often differentially guide specific tasks. Here we explicitly tested whether this discrete perceptual basis contributes to the maintenance of polymorphism in a prey-luring system. The orb-weaving spider Gasteracantha fornicata is known to attract a broad community of primarily dipteran prey due to their conspicuous banded dorsal signal. They occur in two morphs ("white" and "yellow") which should, respectively, generate greater luminance and color contrast in the dipteran eye. Given that arthropods often rely upon luminance-versus-spectral cues for relatively small-versus-large stimulus detection, we predicted a switch in relative attractiveness among morphs according to apparent spider size. Our experimental tests used colour-naïve individuals of two known prey species (Drosophila hydei and Musca domestica) in replicate Y-maze choice trials designed to manipulate the apparent size of spider models via the distance at which they are viewed. Initial trials confirmed that flies were attracted to each G. fornicata morph in single presentations. When given a simultaneous choice between morphs against a viewing background typical of those encountered in nature, flies exhibited no preference regardless of the visual angle subtended by models. However, when backgrounds were adjusted to nearer the extremes of those of each morph in the wild, flies were more attracted by white morphs when presented at longer range (consistent with a reliance on achromatic cues), yet were unbiased in their close-range choice. While not fully consistent with predictions (given the absence of a differential preference for stimuli at close range), our results demonstrate an effect of apparent stimulus size upon relative morph attractiveness in the direction anticipated from present knowledge of fly visual ecology. This implies the potential tuning of G. fornicata morph signal structure according to a perceptual feature that is likely common across their breadth of arthropod prey, and complements recent observational work in suggesting a candidate mechanism for the maintenance of deceptive polymorphism through the exploitation of different visual channels in prey.
Dynamic wake prediction and visualization with uncertainty analysis
NASA Technical Reports Server (NTRS)
Holforty, Wendy L. (Inventor); Powell, J. David (Inventor)
2005-01-01
A dynamic wake avoidance system utilizes aircraft and atmospheric parameters readily available in flight to model and predict airborne wake vortices in real time. A novel combination of algorithms allows for a relatively simple yet robust wake model to be constructed based on information extracted from a broadcast. The system predicts the location and movement of the wake based on the nominal wake model and correspondingly performs an uncertainty analysis on the wake model to determine a wake hazard zone (no fly zone), which comprises a plurality of wake planes, each moving independently from another. The system selectively adjusts dimensions of each wake plane to minimize spatial and temporal uncertainty, thereby ensuring that the actual wake is within the wake hazard zone. The predicted wake hazard zone is communicated in real time directly to a user via a realistic visual representation. In an example, the wake hazard zone is visualized on a 3-D flight deck display to enable a pilot to visualize or see a neighboring aircraft as well as its wake. The system substantially enhances the pilot's situational awareness and allows for a further safe decrease in spacing, which could alleviate airport and airspace congestion.
Neural dynamics for landmark orientation and angular path integration
Seelig, Johannes D.; Jayaraman, Vivek
2015-01-01
Summary Many animals navigate using a combination of visual landmarks and path integration. In mammalian brains, head direction cells integrate these two streams of information by representing an animal's heading relative to landmarks, yet maintaining their directional tuning in darkness based on self-motion cues. Here we use two-photon calcium imaging in head-fixed flies walking on a ball in a virtual reality arena to demonstrate that landmark-based orientation and angular path integration are combined in the population responses of neurons whose dendrites tile the ellipsoid body — a toroidal structure in the center of the fly brain. The population encodes the fly's azimuth relative to its environment, tracking visual landmarks when available and relying on self-motion cues in darkness. When both visual and self-motion cues are absent, a representation of the animal's orientation is maintained in this network through persistent activity — a potential substrate for short-term memory. Several features of the population dynamics of these neurons and their circular anatomical arrangement are suggestive of ring attractors — network structures proposed to support the function of navigational brain circuits. PMID:25971509
Reciprocal Inhibitory Connections Within a Neural Network for Rotational Optic-Flow Processing
Haag, Juergen; Borst, Alexander
2007-01-01
Neurons in the visual system of the blowfly have large receptive fields that are selective for specific optic flow fields. Here, we studied the neural mechanisms underlying flow–field selectivity in proximal Vertical System (VS)-cells, a particular subset of tangential cells in the fly. These cells have local preferred directions that are distributed such as to match the flow field occurring during a rotation of the fly. However, the neural circuitry leading to this selectivity is not fully understood. Through dual intracellular recordings from proximal VS cells and other tangential cells, we characterized the specific wiring between VS cells themselves and between proximal VS cells and horizontal sensitive tangential cells. We discovered a spiking neuron (Vi) involved in this circuitry that has not been described before. This neuron turned out to be connected to proximal VS cells via gap junctions and, in addition, it was found to be inhibitory onto VS1. PMID:18982122
Hardin, P E; Hall, J C; Rosbash, M
1992-01-01
Mutations in the disconnected (disco) gene act to disrupt neural cell patterning in the Drosophila visual system. These mutations also affect adult locomotor activity rhythms, as disco flies are arrhythmic under conditions of constant darkness (DD). To determine the state of the circadian pacemaker in disco mutants, we constructed with pers double mutants (a short period allele of the period gene) and assayed their behavioral rhythms in light-dark cycles (LD), and their biochemical rhythms of period gene expression under both LD and DD conditions. The results demonstrate that disco flies are rhythmic, indicating that they have an active circadian pacemaker that can be entrained by light. They also suggest that disco mutants block or interfere with elements of the circadian system located between the central pacemaker and its outputs that mediate overt rhythms. Images PMID:1740100
Proprioceptive feedback determines visuomotor gain in Drosophila
Bartussek, Jan; Lehmann, Fritz-Olaf
2016-01-01
Multisensory integration is a prerequisite for effective locomotor control in most animals. Especially, the impressive aerial performance of insects relies on rapid and precise integration of multiple sensory modalities that provide feedback on different time scales. In flies, continuous visual signalling from the compound eyes is fused with phasic proprioceptive feedback to ensure precise neural activation of wing steering muscles (WSM) within narrow temporal phase bands of the stroke cycle. This phase-locked activation relies on mechanoreceptors distributed over wings and gyroscopic halteres. Here we investigate visual steering performance of tethered flying fruit flies with reduced haltere and wing feedback signalling. Using a flight simulator, we evaluated visual object fixation behaviour, optomotor altitude control and saccadic escape reflexes. The behavioural assays show an antagonistic effect of wing and haltere signalling on visuomotor gain during flight. Compared with controls, suppression of haltere feedback attenuates while suppression of wing feedback enhances the animal’s wing steering range. Our results suggest that the generation of motor commands owing to visual perception is dynamically controlled by proprioception. We outline a potential physiological mechanism based on the biomechanical properties of WSM and sensory integration processes at the level of motoneurons. Collectively, the findings contribute to our general understanding how moving animals integrate sensory information with dynamically changing temporal structure. PMID:26909184
Ros, Ivo G; Bhagavatula, Partha S; Lin, Huai-Ti; Biewener, Andrew A
2017-02-06
Flying animals must successfully contend with obstacles in their natural environments. Inspired by the robust manoeuvring abilities of flying animals, unmanned aerial systems are being developed and tested to improve flight control through cluttered environments. We previously examined steering strategies that pigeons adopt to fly through an array of vertical obstacles (VOs). Modelling VO flight guidance revealed that pigeons steer towards larger visual gaps when making fast steering decisions. In the present experiments, we recorded three-dimensional flight kinematics of pigeons as they flew through randomized arrays of horizontal obstacles (HOs). We found that pigeons still decelerated upon approach but flew faster through a denser array of HOs compared with the VO array previously tested. Pigeons exhibited limited steering and chose gaps between obstacles most aligned to their immediate flight direction, in contrast to VO navigation that favoured widest gap steering. In addition, pigeons navigated past the HOs with more variable and decreased wing stroke span and adjusted their wing stroke plane to reduce contact with the obstacles. Variability in wing extension, stroke plane and wing stroke path was greater during HO flight. Pigeons also exhibited pronounced head movements when negotiating HOs, which potentially serve a visual function. These head-bobbing-like movements were most pronounced in the horizontal (flight direction) and vertical directions, consistent with engaging motion vision mechanisms for obstacle detection. These results show that pigeons exhibit a keen kinesthetic sense of their body and wings in relation to obstacles. Together with aerodynamic flapping flight mechanics that favours vertical manoeuvring, pigeons are able to navigate HOs using simple rules, with remarkable success.
Borst, Alexander; Weber, Franz
2011-01-01
Optic flow based navigation is a fundamental way of visual course control described in many different species including man. In the fly, an essential part of optic flow analysis is performed in the lobula plate, a retinotopic map of motion in the environment. There, the so-called lobula plate tangential cells possess large receptive fields with different preferred directions in different parts of the visual field. Previous studies demonstrated an extensive connectivity between different tangential cells, providing, in principle, the structural basis for their large and complex receptive fields. We present a network simulation of the tangential cells, comprising most of the neurons studied so far (22 on each hemisphere) with all the known connectivity between them. On their dendrite, model neurons receive input from a retinotopic array of Reichardt-type motion detectors. Model neurons exhibit receptive fields much like their natural counterparts, demonstrating that the connectivity between the lobula plate tangential cells indeed can account for their complex receptive field structure. We describe the tuning of a model neuron to particular types of ego-motion (rotation as well as translation around/along a given body axis) by its ‘action field’. As we show for model neurons of the vertical system (VS-cells), each of them displays a different type of action field, i.e., responds maximally when the fly is rotating around a particular body axis. However, the tuning width of the rotational action fields is relatively broad, comparable to the one with dendritic input only. The additional intra-lobula-plate connectivity mainly reduces their translational action field amplitude, i.e., their sensitivity to translational movements along any body axis of the fly. PMID:21305019
Ros, Ivo G.; Bhagavatula, Partha S.; Lin, Huai-Ti
2017-01-01
Flying animals must successfully contend with obstacles in their natural environments. Inspired by the robust manoeuvring abilities of flying animals, unmanned aerial systems are being developed and tested to improve flight control through cluttered environments. We previously examined steering strategies that pigeons adopt to fly through an array of vertical obstacles (VOs). Modelling VO flight guidance revealed that pigeons steer towards larger visual gaps when making fast steering decisions. In the present experiments, we recorded three-dimensional flight kinematics of pigeons as they flew through randomized arrays of horizontal obstacles (HOs). We found that pigeons still decelerated upon approach but flew faster through a denser array of HOs compared with the VO array previously tested. Pigeons exhibited limited steering and chose gaps between obstacles most aligned to their immediate flight direction, in contrast to VO navigation that favoured widest gap steering. In addition, pigeons navigated past the HOs with more variable and decreased wing stroke span and adjusted their wing stroke plane to reduce contact with the obstacles. Variability in wing extension, stroke plane and wing stroke path was greater during HO flight. Pigeons also exhibited pronounced head movements when negotiating HOs, which potentially serve a visual function. These head-bobbing-like movements were most pronounced in the horizontal (flight direction) and vertical directions, consistent with engaging motion vision mechanisms for obstacle detection. These results show that pigeons exhibit a keen kinesthetic sense of their body and wings in relation to obstacles. Together with aerodynamic flapping flight mechanics that favours vertical manoeuvring, pigeons are able to navigate HOs using simple rules, with remarkable success. PMID:28163883
A bee in the corridor: centering and wall-following
NASA Astrophysics Data System (ADS)
Serres, Julien R.; Masson, Guillaume P.; Ruffier, Franck; Franceschini, Nicolas
2008-12-01
In an attempt to better understand the mechanism underlying lateral collision avoidance in flying insects, we trained honeybees ( Apis mellifera) to fly through a large (95-cm wide) flight tunnel. We found that, depending on the entrance and feeder positions, honeybees would either center along the corridor midline or fly along one wall. Bees kept following one wall even when a major (150-cm long) part of the opposite wall was removed. These findings cannot be accounted for by the “optic flow balance” hypothesis that has been put forward to explain the typical bees’ “centering response” observed in narrower corridors. Both centering and wall-following behaviors are well accounted for, however, by a control scheme called the lateral optic flow regulator, i.e., a feedback system that strives to maintain the unilateral optic flow constant. The power of this control scheme is that it would allow the bee to guide itself visually in a corridor without having to measure its speed or distance from the walls.
The Function and Organization of the Motor System Controlling Flight Maneuvers in Flies.
Lindsay, Theodore; Sustar, Anne; Dickinson, Michael
2017-02-06
Animals face the daunting task of controlling their limbs using a small set of highly constrained actuators. This problem is particularly demanding for insects such as Drosophila, which must adjust wing motion for both quick voluntary maneuvers and slow compensatory reflexes using only a dozen pairs of muscles. To identify strategies by which animals execute precise actions using sparse motor networks, we imaged the activity of a complete ensemble of wing control muscles in intact, flying flies. Our experiments uncovered a remarkably efficient logic in which each of the four skeletal elements at the base of the wing are equipped with both large phasically active muscles capable of executing large changes and smaller tonically active muscles specialized for continuous fine-scaled adjustments. Based on the responses to a broad panel of visual motion stimuli, we have developed a model by which the motor array regulates aerodynamically functional features of wing motion. VIDEO ABSTRACT. Copyright © 2017 Elsevier Ltd. All rights reserved.
GenColors-based comparative genome databases for small eukaryotic genomes.
Felder, Marius; Romualdi, Alessandro; Petzold, Andreas; Platzer, Matthias; Sühnel, Jürgen; Glöckner, Gernot
2013-01-01
Many sequence data repositories can give a quick and easily accessible overview on genomes and their annotations. Less widespread is the possibility to compare related genomes with each other in a common database environment. We have previously described the GenColors database system (http://gencolors.fli-leibniz.de) and its applications to a number of bacterial genomes such as Borrelia, Legionella, Leptospira and Treponema. This system has an emphasis on genome comparison. It combines data from related genomes and provides the user with an extensive set of visualization and analysis tools. Eukaryote genomes are normally larger than prokaryote genomes and thus pose additional challenges for such a system. We have, therefore, adapted GenColors to also handle larger datasets of small eukaryotic genomes and to display eukaryotic gene structures. Further recent developments include whole genome views, genome list options and, for bacterial genome browsers, the display of horizontal gene transfer predictions. Two new GenColors-based databases for two fungal species (http://fgb.fli-leibniz.de) and for four social amoebas (http://sacgb.fli-leibniz.de) were set up. Both new resources open up a single entry point for related genomes for the amoebozoa and fungal research communities and other interested users. Comparative genomics approaches are greatly facilitated by these resources.
Egelhaaf, Martin; Kern, Roland
2002-12-01
Vision guides flight behaviour in numerous insects. Despite their small brain, insects easily outperform current man-made autonomous vehicles in many respects. Examples are the virtuosic chasing manoeuvres male flies perform as part of their mating behaviour and the ability of bees to assess, on the basis of visual motion cues, the distance travelled in a novel environment. Analyses at both the behavioural and neuronal levels are beginning to unveil reasons for such extraordinary capabilities of insects. One recipe for their success is the adaptation of visual information processing to the specific requirements of the behavioural tasks and to the specific spatiotemporal properties of the natural input.
DOT National Transportation Integrated Search
1978-03-01
At night, reduced visual cues may promote illusions and a dangerous tendency for pilots to fly low during approaches to landing. Relative motion parallax (a difference in rate of apparent movement of objects in the visual field), a cue that can contr...
Mechanisms of Photoreceptor Patterning in Vertebrates and Invertebrates.
Viets, Kayla; Eldred, Kiara; Johnston, Robert J
2016-10-01
Across the animal kingdom, visual systems have evolved to be uniquely suited to the environments and behavioral patterns of different species. Visual acuity and color perception depend on the distribution of photoreceptor (PR) subtypes within the retina. Retinal mosaics can be organized into three broad categories: stochastic/regionalized, regionalized, and ordered. We describe here the retinal mosaics of flies, zebrafish, chickens, mice, and humans, and the gene regulatory networks controlling proper PR specification in each. By drawing parallels in eye development between these divergent species, we identify a set of conserved organizing principles and transcriptional networks that govern PR subtype differentiation. Copyright © 2016 Elsevier Ltd. All rights reserved.
Mechanisms of photoreceptor patterning in vertebrates and invertebrates
Johnston, Robert J
2016-01-01
Across the animal kingdom, visual systems have evolved to be uniquely suited to the environments and behavioral patterns of different species. The visual acuity and color perception of organisms depend on the distribution of photoreceptor subtypes within the retina. Retinal mosaics can be organized into three broad categories: stochastic/regionalized, regionalized, and ordered. Here, we describe the retinal mosaics of flies, zebrafish, chickens, mice, and humans and the gene regulatory networks controlling proper photoreceptor specification in each. By drawing parallels in eye development between these divergent species, we identify a set of conserved organizing principles and transcriptional networks that govern photoreceptor subtype differentiation. PMID:27615122
Narendra, Ajay; Ramirez-Esquivel, Fiorella; Ribi, Willi A.
2016-01-01
Ants are unusual among insects in that individuals of the same species within a single colony have different modes of locomotion and tasks. We know from walking ants that vision plays a significant role in guiding this behaviour, but we know surprisingly little about the potential contribution of visual sensory structures for a flying mode of locomotion. Here we investigate the structure of the compound eye and ocelli in pedestrian workers, alate females and alate males of an Australian ant, Camponotus consobrinus, and discuss the trade-offs involved in optical sensitivity and spatial resolution. Male ants have more but smaller ommatidia and the smallest interommatidial angles, which is most likely an adaptation to visually track individual flying females. Both walking and flying forms of ants have a similar proportion of specialized receptors sensitive to polarized skylight, but the absolute number of these receptors varies, being greatest in males. Ocelli are present only in the flying forms. Each ocellus consists of a bipartite retina with a horizon-facing dorsal retina, which contains retinula cells with long rhabdoms, and a sky-facing ventral retina with shorter rhabdoms. We discuss the implications of these and their potential for sensing the pattern of polarized skylight. PMID:26975481
Narendra, Ajay; Ramirez-Esquivel, Fiorella; Ribi, Willi A
2016-03-15
Ants are unusual among insects in that individuals of the same species within a single colony have different modes of locomotion and tasks. We know from walking ants that vision plays a significant role in guiding this behaviour, but we know surprisingly little about the potential contribution of visual sensory structures for a flying mode of locomotion. Here we investigate the structure of the compound eye and ocelli in pedestrian workers, alate females and alate males of an Australian ant, Camponotus consobrinus, and discuss the trade-offs involved in optical sensitivity and spatial resolution. Male ants have more but smaller ommatidia and the smallest interommatidial angles, which is most likely an adaptation to visually track individual flying females. Both walking and flying forms of ants have a similar proportion of specialized receptors sensitive to polarized skylight, but the absolute number of these receptors varies, being greatest in males. Ocelli are present only in the flying forms. Each ocellus consists of a bipartite retina with a horizon-facing dorsal retina, which contains retinula cells with long rhabdoms, and a sky-facing ventral retina with shorter rhabdoms. We discuss the implications of these and their potential for sensing the pattern of polarized skylight.
Virtual-reality techniques resolve the visual cues used by fruit flies to evaluate object distances.
Schuster, Stefan; Strauss, Roland; Götz, Karl G
2002-09-17
Insects can estimate distance or time-to-contact of surrounding objects from locomotion-induced changes in their retinal position and/or size. Freely walking fruit flies (Drosophila melanogaster) use the received mixture of different distance cues to select the nearest objects for subsequent visits. Conventional methods of behavioral analysis fail to elucidate the underlying data extraction. Here we demonstrate first comprehensive solutions of this problem by substituting virtual for real objects; a tracker-controlled 360 degrees panorama converts a fruit fly's changing coordinates into object illusions that require the perception of specific cues to appear at preselected distances up to infinity. An application reveals the following: (1) en-route sampling of retinal-image changes accounts for distance discrimination within a surprising range of at least 8-80 body lengths (20-200 mm). Stereopsis and peering are not involved. (2) Distance from image translation in the expected direction (motion parallax) outweighs distance from image expansion, which accounts for impact-avoiding flight reactions to looming objects. (3) The ability to discriminate distances is robust to artificially delayed updating of image translation. Fruit flies appear to interrelate self-motion and its visual feedback within a surprisingly long time window of about 2 s. The comparative distance inspection practiced in the small fruit fly deserves utilization in self-moving robots.
Improving Aviation Safety with information Visualization: A Flight Simulation Study
NASA Technical Reports Server (NTRS)
Aragon, Cecilia R.; Hearst, Marti
2005-01-01
Many aircraft accidents each year are caused by encounters with invisible airflow hazards. Recent advances in aviation sensor technology offer the potential for aircraft-based sensors that can gather large amounts of airflow velocity data in real-time. With this influx of data comes the need to study how best to present it to the pilot - a cognitively overloaded user focused on a primary task other than that of information visualization. In this paper, we present the results of a usability study of an airflow hazard visualization system that significantly reduced the crash rate among experienced helicopter pilots flying a high fidelity, aerodynamically realistic fixed-base rotorcraft flight simulator into hazardous conditions. We focus on one particular aviation application, but the results may be relevant to user interfaces in other operationally stressful environments.
Neurons Forming Optic Glomeruli Compute Figure–Ground Discriminations in Drosophila
Aptekar, Jacob W.; Keleş, Mehmet F.; Lu, Patrick M.; Zolotova, Nadezhda M.
2015-01-01
Many animals rely on visual figure–ground discrimination to aid in navigation, and to draw attention to salient features like conspecifics or predators. Even figures that are similar in pattern and luminance to the visual surroundings can be distinguished by the optical disparity generated by their relative motion against the ground, and yet the neural mechanisms underlying these visual discriminations are not well understood. We show in flies that a diverse array of figure–ground stimuli containing a motion-defined edge elicit statistically similar behavioral responses to one another, and statistically distinct behavioral responses from ground motion alone. From studies in larger flies and other insect species, we hypothesized that the circuitry of the lobula—one of the four, primary neuropiles of the fly optic lobe—performs this visual discrimination. Using calcium imaging of input dendrites, we then show that information encoded in cells projecting from the lobula to discrete optic glomeruli in the central brain group these sets of figure–ground stimuli in a homologous manner to the behavior; “figure-like” stimuli are coded similar to one another and “ground-like” stimuli are encoded differently. One cell class responds to the leading edge of a figure and is suppressed by ground motion. Two other classes cluster any figure-like stimuli, including a figure moving opposite the ground, distinctly from ground alone. This evidence demonstrates that lobula outputs provide a diverse basis set encoding visual features necessary for figure detection. PMID:25972183
Neurons forming optic glomeruli compute figure-ground discriminations in Drosophila.
Aptekar, Jacob W; Keleş, Mehmet F; Lu, Patrick M; Zolotova, Nadezhda M; Frye, Mark A
2015-05-13
Many animals rely on visual figure-ground discrimination to aid in navigation, and to draw attention to salient features like conspecifics or predators. Even figures that are similar in pattern and luminance to the visual surroundings can be distinguished by the optical disparity generated by their relative motion against the ground, and yet the neural mechanisms underlying these visual discriminations are not well understood. We show in flies that a diverse array of figure-ground stimuli containing a motion-defined edge elicit statistically similar behavioral responses to one another, and statistically distinct behavioral responses from ground motion alone. From studies in larger flies and other insect species, we hypothesized that the circuitry of the lobula--one of the four, primary neuropiles of the fly optic lobe--performs this visual discrimination. Using calcium imaging of input dendrites, we then show that information encoded in cells projecting from the lobula to discrete optic glomeruli in the central brain group these sets of figure-ground stimuli in a homologous manner to the behavior; "figure-like" stimuli are coded similar to one another and "ground-like" stimuli are encoded differently. One cell class responds to the leading edge of a figure and is suppressed by ground motion. Two other classes cluster any figure-like stimuli, including a figure moving opposite the ground, distinctly from ground alone. This evidence demonstrates that lobula outputs provide a diverse basis set encoding visual features necessary for figure detection. Copyright © 2015 the authors 0270-6474/15/357587-13$15.00/0.
Linander, Nellie; Baird, Emily; Dacke, Marie
2017-05-01
Flying insects frequently navigate through environments of different complexity. In this study, buff-tailed bumblebees (Bombus terrestris L.) were trained to fly along tunnels of different widths, from 60 to 240 cm. In tunnel widths of 60 and 120 cm, bumblebees control their lateral position by balancing the magnitude of translational optic flow experienced in the lateral visual field of each eye. In wider tunnels, bumblebees use translational optic flow cues in the ventral visual field to control their lateral position and to steer along straight tracks. Our results also suggest that bumblebees prefer to fly over surfaces that provide strong ventral optic flow cues, rather than over featureless ones. Together, these strategies allow bumblebees to minimize the risk of collision and to maintain relatively straight flight paths in a broad range of environments.
Drosophila increase exploration after visually detecting predators.
de la Flor, Miguel; Chen, Lijian; Manson-Bishop, Claire; Chu, Tzu-Chun; Zamora, Kathya; Robbins, Danielle; Gunaratne, Gemunu; Roman, Gregg
2017-01-01
Novel stimuli elicit behaviors that are collectively known as specific exploration. These behaviors allow the animal to become more familiar with the novel objects within its environment. Specific exploration is frequently suppressed by defensive reactions to predator cues. Herein, we examine if this suppression occurs in Drosophila melanogaster by measuring the response of these flies to wild harvested predators. The flies used in our experiments have been cultured and had not lived under predator threat for multiple decades. In a circular arena with centrally-caged predators, wild type Drosophila actively avoided the pantropical jumping spider, Plexippus paykulli, and the Texas unicorn mantis, Phyllovates chlorophaena, indicating an innate defensive reaction to these predators. Interestingly, wild type Drosophila males also avoided a centrally-caged mock spider, and the avoidance of the mock spider became exaggerated when it was made to move within the cage. Visually impaired Drosophila failed to detect and avoid the Plexippus paykulli and the moving mock spider, while the broadly anosmic orco2 mutants were fully capable of detecting and avoiding Plexippus paykulli, indicating that these flies principally relied upon vison to perceive the predator stimuli. During early exploration of the arena, exploratory activity increased in the presence of Plexippus paykulli and the moving mock spider. The elevated activity induced by Plexippus paykulli disappeared after the fly had finished exploring, suggesting the flies were capable of habituating the predator cues. Taken together, these results indicate that despite being isolated from predators for decades Drosophila will visually detect these predators, retain innate defensive behaviors, respond by increasing exploratory activity in the arena rather than suppressing activity, and may habituate to normal predator cues.
Drosophila increase exploration after visually detecting predators
Manson-Bishop, Claire; Chu, Tzu-Chun; Zamora, Kathya; Robbins, Danielle; Gunaratne, Gemunu
2017-01-01
Novel stimuli elicit behaviors that are collectively known as specific exploration. These behaviors allow the animal to become more familiar with the novel objects within its environment. Specific exploration is frequently suppressed by defensive reactions to predator cues. Herein, we examine if this suppression occurs in Drosophila melanogaster by measuring the response of these flies to wild harvested predators. The flies used in our experiments have been cultured and had not lived under predator threat for multiple decades. In a circular arena with centrally-caged predators, wild type Drosophila actively avoided the pantropical jumping spider, Plexippus paykulli, and the Texas unicorn mantis, Phyllovates chlorophaena, indicating an innate defensive reaction to these predators. Interestingly, wild type Drosophila males also avoided a centrally-caged mock spider, and the avoidance of the mock spider became exaggerated when it was made to move within the cage. Visually impaired Drosophila failed to detect and avoid the Plexippus paykulli and the moving mock spider, while the broadly anosmic orco2 mutants were fully capable of detecting and avoiding Plexippus paykulli, indicating that these flies principally relied upon vison to perceive the predator stimuli. During early exploration of the arena, exploratory activity increased in the presence of Plexippus paykulli and the moving mock spider. The elevated activity induced by Plexippus paykulli disappeared after the fly had finished exploring, suggesting the flies were capable of habituating the predator cues. Taken together, these results indicate that despite being isolated from predators for decades Drosophila will visually detect these predators, retain innate defensive behaviors, respond by increasing exploratory activity in the arena rather than suppressing activity, and may habituate to normal predator cues. PMID:28746346
A Model for the Detection of Moving Targets in Visual Clutter Inspired by Insect Physiology
2008-07-01
paper: SDW PS DCO. References 1. Wagner H (1986) Flight performance and visual control of flight of the free- flying housefly (Musca domestica L) 3...differences in the chasing behaviour of houseflies (musca). Biol Cybern 32: 239–241. 3. Land MF (1997) Visual acuity in insects. Annu Rev Entomol 42: 147
ERIC Educational Resources Information Center
Pan, Yufeng; Zhou, Yanqiong; Guo, Chao; Gong, Haiyun; Gong, Zhefeng; Liu, Li
2009-01-01
The central complex is a prominent structure in the "Drosophila" brain. Visual learning experiments in the flight simulator, with flies with genetically altered brains, revealed that two groups of horizontal neurons in one of its substructures, the fan-shaped body, were required for "Drosophila" visual pattern memory. However,…
USDA-ARS?s Scientific Manuscript database
We report on a pearly eye mutant (PEM) line generated from a single male Bactrocera cucurbitae collected in Kapoho, Hawaii. Crossing experiments with colony wild-type flies indicate that the locus controlling this trait is autosomal and the mutant allele is recessive. Experiments with females to ass...
Garcia-Cantero, Juan J; Brito, Juan P; Mata, Susana; Bayona, Sofia; Pastor, Luis
2017-01-01
Gaining a better understanding of the human brain continues to be one of the greatest challenges for science, largely because of the overwhelming complexity of the brain and the difficulty of analyzing the features and behavior of dense neural networks. Regarding analysis, 3D visualization has proven to be a useful tool for the evaluation of complex systems. However, the large number of neurons in non-trivial circuits, together with their intricate geometry, makes the visualization of a neuronal scenario an extremely challenging computational problem. Previous work in this area dealt with the generation of 3D polygonal meshes that approximated the cells' overall anatomy but did not attempt to deal with the extremely high storage and computational cost required to manage a complex scene. This paper presents NeuroTessMesh, a tool specifically designed to cope with many of the problems associated with the visualization of neural circuits that are comprised of large numbers of cells. In addition, this method facilitates the recovery and visualization of the 3D geometry of cells included in databases, such as NeuroMorpho, and provides the tools needed to approximate missing information such as the soma's morphology. This method takes as its only input the available compact, yet incomplete, morphological tracings of the cells as acquired by neuroscientists. It uses a multiresolution approach that combines an initial, coarse mesh generation with subsequent on-the-fly adaptive mesh refinement stages using tessellation shaders. For the coarse mesh generation, a novel approach, based on the Finite Element Method, allows approximation of the 3D shape of the soma from its incomplete description. Subsequently, the adaptive refinement process performed in the graphic card generates meshes that provide good visual quality geometries at a reasonable computational cost, both in terms of memory and rendering time. All the described techniques have been integrated into NeuroTessMesh, available to the scientific community, to generate, visualize, and save the adaptive resolution meshes.
Predictive Coding: A Fresh View of Inhibition in the Retina
NASA Astrophysics Data System (ADS)
Srinivasan, M. V.; Laughlin, S. B.; Dubs, A.
1982-11-01
Interneurons exhibiting centre--surround antagonism within their receptive fields are commonly found in peripheral visual pathways. We propose that this organization enables the visual system to encode spatial detail in a manner that minimizes the deleterious effects of intrinsic noise, by exploiting the spatial correlation that exists within natural scenes. The antagonistic surround takes a weighted mean of the signals in neighbouring receptors to generate a statistical prediction of the signal at the centre. The predicted value is subtracted from the actual centre signal, thus minimizing the range of outputs transmitted by the centre. In this way the entire dynamic range of the interneuron can be devoted to encoding a small range of intensities, thus rendering fine detail detectable against intrinsic noise injected at later stages in processing. This predictive encoding scheme also reduces spatial redundancy, thereby enabling the array of interneurons to transmit a larger number of distinguishable images, taking into account the expected structure of the visual world. The profile of the required inhibitory field is derived from statistical estimation theory. This profile depends strongly upon the signal: noise ratio and weakly upon the extent of lateral spatial correlation. The receptive fields that are quantitatively predicted by the theory resemble those of X-type retinal ganglion cells and show that the inhibitory surround should become weaker and more diffuse at low intensities. The latter property is unequivocally demonstrated in the first-order interneurons of the fly's compound eye. The theory is extended to the time domain to account for the phasic responses of fly interneurons. These comparisons suggest that, in the early stages of processing, the visual system is concerned primarily with coding the visual image to protect against subsequent intrinsic noise, rather than with reconstructing the scene or extracting specific features from it. The treatment emphasizes that a neuron's dynamic range should be matched to both its receptive field and the statistical properties of the visual pattern expected within this field. Finally, the analysis is synthetic because it is an extension of the background suppression hypothesis (Barlow & Levick 1976), satisfies the redundancy reduction hypothesis (Barlow 1961 a, b) and is equivalent to deblurring under certain conditions (Ratliff 1965).
Explorative visual analytics on interval-based genomic data and their metadata.
Jalili, Vahid; Matteucci, Matteo; Masseroli, Marco; Ceri, Stefano
2017-12-04
With the wide-spreading of public repositories of NGS processed data, the availability of user-friendly and effective tools for data exploration, analysis and visualization is becoming very relevant. These tools enable interactive analytics, an exploratory approach for the seamless "sense-making" of data through on-the-fly integration of analysis and visualization phases, suggested not only for evaluating processing results, but also for designing and adapting NGS data analysis pipelines. This paper presents abstractions for supporting the early analysis of NGS processed data and their implementation in an associated tool, named GenoMetric Space Explorer (GeMSE). This tool serves the needs of the GenoMetric Query Language, an innovative cloud-based system for computing complex queries over heterogeneous processed data. It can also be used starting from any text files in standard BED, BroadPeak, NarrowPeak, GTF, or general tab-delimited format, containing numerical features of genomic regions; metadata can be provided as text files in tab-delimited attribute-value format. GeMSE allows interactive analytics, consisting of on-the-fly cycling among steps of data exploration, analysis and visualization that help biologists and bioinformaticians in making sense of heterogeneous genomic datasets. By means of an explorative interaction support, users can trace past activities and quickly recover their results, seamlessly going backward and forward in the analysis steps and comparative visualizations of heatmaps. GeMSE effective application and practical usefulness is demonstrated through significant use cases of biological interest. GeMSE is available at http://www.bioinformatics.deib.polimi.it/GeMSE/ , and its source code is available at https://github.com/Genometric/GeMSE under GPLv3 open-source license.
Potentiation in the first visual synapse of the fly compound eye.
Uusitalo, R O; Weckström, M
2000-04-01
In the first visual synapse of the insect compound eye, both the presynaptic and postsynaptic signals are graded, nonspiking changes in membrane voltage. The synapse exhibits tonic transmitter release (even in dark) and strong adaptation to long-lasting light backgrounds, leading to changes also in the dynamics of signal transmission. We have studied these adaptational properties of the first visual synapse of the blowfly Calliphora vicina. Investigations were done in situ by intracellular recordings from the presynaptic photoreceptors, photoreceptor axon terminals, and the postsynaptic first order visual interneurons (LMCs). The dark recovery, the shifts in intensity dependence, and the underlying processes were studied by stimulating the visual system with various adapting stimuli while observing the recovery (i.e., dark adaptation). The findings show a transient potentiation in the postsynaptic responses after intense light adaptation, and the underlying mechanisms seem to be the changes in the equilibrium potential of the transmitter-gated conductance (chloride) of the postsynaptic neurons. The potentiation by itself serves as a mechanism that after light adaptation rapidly recovers the sensitivity loss of the visual system. However, this kind of mechanism, being an intrinsic property of graded potential transmission, may be quite widespread among graded synapses, and the phenomenon demonstrates that functional plasticity is also a property of graded synaptic transmission.
NASA Astrophysics Data System (ADS)
Wood, Brian M.; Wood, Zoë J.
2006-01-01
We present a visualization and computation tool for modeling the caloric cost of pedestrian travel across three dimensional terrains. This tool is being used in ongoing archaeological research that analyzes how costs of locomotion affect the spatial distribution of trails and artifacts across archaeological landscapes. Throughout human history, traveling by foot has been the most common form of transportation, and therefore analyses of pedestrian travel costs are important for understanding prehistoric patterns of resource acquisition, migration, trade, and political interaction. Traditionally, archaeologists have measured geographic proximity based on "as the crow flies" distance. We propose new methods for terrain visualization and analysis based on measuring paths of least caloric expense, calculated using well established metabolic equations. Our approach provides a human centered metric of geographic closeness, and overcomes significant limitations of available Geographic Information System (GIS) software. We demonstrate such path computations and visualizations applied to archaeological research questions. Our system includes tools to visualize: energetic cost surfaces, comparisons of the elevation profiles of shortest paths versus least cost paths, and the display of paths of least caloric effort on Digital Elevation Models (DEMs). These analysis tools can be applied to calculate and visualize 1) likely locations of prehistoric trails and 2) expected ratios of raw material types to be recovered at archaeological sites.
Horowitz, Seth S; Cheney, Cheryl A; Simmons, James A
2004-01-01
The big brown bat (Eptesicus fuscus) is an aerial-feeding insectivorous species that relies on echolocation to avoid obstacles and to detect flying insects. Spatial perception in the dark using echolocation challenges the vestibular system to function without substantial visual input for orientation. IR thermal video recordings show the complexity of bat flights in the field and suggest a highly dynamic role for the vestibular system in orientation and flight control. To examine this role, we carried out laboratory studies of flight behavior under illuminated and dark conditions in both static and rotating obstacle tests while administering heavy water (D2O) to impair vestibular inputs. Eptesicus carried out complex maneuvers through both fixed arrays of wires and a rotating obstacle array using both vision and echolocation, or when guided by echolocation alone. When treated with D2O in combination with lack of visual cues, bats showed considerable decrements in performance. These data indicate that big brown bats use both vision and echolocation to provide spatial registration for head position information generated by the vestibular system.
ERIC Educational Resources Information Center
Benard, Julie; Giurfa, Martin
2004-01-01
We asked whether honeybees, "Apis mellifera," could solve a transitive inference problem. Individual free-flying bees were conditioned with four overlapping premise pairs of five visual patterns in a multiple discrimination task (A+ vs. B-, B+ vs. C-, C+ vs. D-, D+ vs. E-, where + and - indicate sucrose reward or absence of it,…
Collision-avoidance behaviors of minimally restrained flying locusts to looming stimuli
Chan, R. WM.; Gabbiani, F.
2013-01-01
SUMMARY Visually guided collision avoidance is of paramount importance in flight, for instance to allow escape from potential predators. Yet, little is known about the types of collision-avoidance behaviors that may be generated by flying animals in response to an impending visual threat. We studied the behavior of minimally restrained locusts flying in a wind tunnel as they were subjected to looming stimuli presented to the side of the animal, simulating the approach of an object on a collision course. Using high-speed movie recordings, we observed a wide variety of collision-avoidance behaviors including climbs and dives away from – but also towards – the stimulus. In a more restrained setting, we were able to relate kinematic parameters of the flapping wings with yaw changes in the trajectory of the animal. Asymmetric wing flapping was most strongly correlated with changes in yaw, but we also observed a substantial effect of wing deformations. Additionally, the effect of wing deformations on yaw was relatively independent of that of wing asymmetries. Thus, flying locusts exhibit a rich range of collision-avoidance behaviors that depend on several distinct aerodynamic characteristics of wing flapping flight. PMID:23364572
Neural control and precision of flight muscle activation in Drosophila.
Lehmann, Fritz-Olaf; Bartussek, Jan
2017-01-01
Precision of motor commands is highly relevant in a large context of various locomotor behaviors, including stabilization of body posture, heading control and directed escape responses. While posture stability and heading control in walking and swimming animals benefit from high friction via ground reaction forces and elevated viscosity of water, respectively, flying animals have to cope with comparatively little aerodynamic friction on body and wings. Although low frictional damping in flight is the key to the extraordinary aerial performance and agility of flying birds, bats and insects, it challenges these animals with extraordinary demands on sensory integration and motor precision. Our review focuses on the dynamic precision with which Drosophila activates its flight muscular system during maneuvering flight, considering relevant studies on neural and muscular mechanisms of thoracic propulsion. In particular, we tackle the precision with which flies adjust power output of asynchronous power muscles and synchronous flight control muscles by monitoring muscle calcium and spike timing within the stroke cycle. A substantial proportion of the review is engaged in the significance of visual and proprioceptive feedback loops for wing motion control including sensory integration at the cellular level. We highlight that sensory feedback is the basis for precise heading control and body stability in flies.
Schwegmann, Alexander; Lindemann, Jens Peter; Egelhaaf, Martin
2014-01-01
Many flying insects, such as flies, wasps and bees, pursue a saccadic flight and gaze strategy. This behavioral strategy is thought to separate the translational and rotational components of self-motion and, thereby, to reduce the computational efforts to extract information about the environment from the retinal image flow. Because of the distinguishing dynamic features of this active flight and gaze strategy of insects, the present study analyzes systematically the spatiotemporal statistics of image sequences generated during saccades and intersaccadic intervals in cluttered natural environments. We show that, in general, rotational movements with saccade-like dynamics elicit fluctuations and overall changes in brightness, contrast and spatial frequency of up to two orders of magnitude larger than translational movements at velocities that are characteristic of insects. Distinct changes in image parameters during translations are only caused by nearby objects. Image analysis based on larger patches in the visual field reveals smaller fluctuations in brightness and spatial frequency composition compared to small patches. The temporal structure and extent of these changes in image parameters define the temporal constraints imposed on signal processing performed by the insect visual system under behavioral conditions in natural environments. PMID:25340761
[Causes of fatigue in civil aviation helicopter crews].
Kamenskiĭ, Iu N
1981-01-01
Vibration and noise play an important part in fatiguing crewmembers of helicopters. The exposure to these factors during the flying shift results in an early and marked fatigue of pilots the level of which depends on the vibration effects to a larger extent than on the noise effects. The fatigue is followed by a decline of the psychophysiological parameters characterizing the visual and motor functions as well as the ratio of the basic processes in the central nervous system.
Independent Research and Independent Exploratory Development FY 1985
1986-01-01
whs’tkxwK 3nk ’ --’ ." -•ftFiu... imcnft fli m le- Apanl these individua complete * hic tm rmthstst h frttw tpsi hesltin ndte the correct answer (33%) were...describe itself, graphically indicating a Maintenance System ( TMS ), which design relationship, or examining a maintains a set of assumptions within an...critique comment by visually marking environment. At this point, how to utilize the elements involved, a TMS within Designer has not been clearly , defined
NASA Astrophysics Data System (ADS)
Moody, Marc; Fisher, Robert; Little, J. Kristin
2014-06-01
Boeing has developed a degraded visual environment navigational aid that is flying on the Boeing AH-6 light attack helicopter. The navigational aid is a two dimensional software digital map underlay generated by the Boeing™ Geospatial Embedded Mapping Software (GEMS) and fully integrated with the operational flight program. The page format on the aircraft's multi function displays (MFD) is termed the Approach page. The existing work utilizes Digital Terrain Elevation Data (DTED) and OpenGL ES 2.0 graphics capabilities to compute the pertinent graphics underlay entirely on the graphics processor unit (GPU) within the AH-6 mission computer. The next release will incorporate cultural databases containing Digital Vertical Obstructions (DVO) to warn the crew of towers, buildings, and power lines when choosing an opportune landing site. Future IRAD will include Light Detection and Ranging (LIDAR) point cloud generating sensors to provide 2D and 3D synthetic vision on the final approach to the landing zone. Collision detection with respect to terrain, cultural, and point cloud datasets may be used to further augment the crew warning system. The techniques for creating the digital map underlay leverage the GPU almost entirely, making this solution viable on most embedded mission computing systems with an OpenGL ES 2.0 capable GPU. This paper focuses on the AH-6 crew interface process for determining a landing zone and flying the aircraft to it.
Oonincx, D G A B; Volk, N; Diehl, J J E; van Loon, J J A; Belušič, G
2016-12-01
Mating in the black soldier fly (BSF) is a visually mediated behaviour that under natural conditions occurs in full sunlight. Artificial light conditions promoting mating by BSF were designed based on the spectral characteristics of the compound eye retina. Electrophysiological measurements revealed that BSF ommatidia contained UV-, blue- and green-sensitive photoreceptor cells, allowing trichromatic vision. An illumination system for indoor breeding based on UV, blue and green LEDs was designed and its efficiency was compared with illumination by fluorescent tubes which have been successfully used to sustain a BSF colony for five years. Illumination by LEDs and the fluorescent tubes yielded equal numbers of egg clutches, however, the LED illumination resulted in significantly more larvae. The possibilities to optimize the current LED illumination system to better approximate the skylight illuminant and potentially optimize the larval yield are discussed. Copyright © 2016 Elsevier Ltd. All rights reserved.
Dendro-dendritic interactions between motion-sensitive large-field neurons in the fly.
Haag, Juergen; Borst, Alexander
2002-04-15
For visual course control, flies rely on a set of motion-sensitive neurons called lobula plate tangential cells (LPTCs). Among these cells, the so-called CH (centrifugal horizontal) cells shape by their inhibitory action the receptive field properties of other LPTCs called FD (figure detection) cells specialized for figure-ground discrimination based on relative motion. Studying the ipsilateral input circuitry of CH cells by means of dual-electrode and combined electrical-optical recordings, we find that CH cells receive graded input from HS (large-field horizontal system) cells via dendro-dendritic electrical synapses. This particular wiring scheme leads to a spatial blur of the motion image on the CH cell dendrite, and, after inhibiting FD cells, to an enhancement of motion contrast. This could be crucial for enabling FD cells to discriminate object from self motion.
Localization from Visual Landmarks on a Free-Flying Robot
NASA Technical Reports Server (NTRS)
Coltin, Brian; Fusco, Jesse; Moratto, Zack; Alexandrov, Oleg; Nakamura, Robert
2016-01-01
We present the localization approach for Astrobee,a new free-flying robot designed to navigate autonomously on board the International Space Station (ISS). Astrobee will conduct experiments in microgravity, as well as assisst astronauts and ground controllers. Astrobee replaces the SPHERES robots which currently operate on the ISS, which were limited to operating in a small cube since their localization system relied on triangulation from ultrasonic transmitters. Astrobee localizes with only monocular vision and an IMU, enabling it to traverse the entire US segment of the station. Features detected on a previously-built map, optical flow information,and IMU readings are all integrated into an extended Kalman filter (EKF) to estimate the robot pose. We introduce several modifications to the filter to make it more robust to noise.Finally, we extensively evaluate the behavior of the filter on atwo-dimensional testing surface.
Visual Spatial Disorientation: Re-Visiting the Black Hole Illusion
2007-01-24
National Transportation Safety Board. Controlled Flight into Terrain, Korean Air Flight 801 , Nimitz Hill, Guam; 1997. 50. National Transportation Safety...According to a Boeing study of worldwide commercial airline accidents, the approach and landing phase of flying, although only accounting for 4% of the...VISUAL SPATIAL DISORIENTATION Kraft (31) described four night visual, landing airline accidents. Black Hole Illusion 5 1. In 1965, a United Airlines
Gerry, Alec C; Higginbotham, G E; Periera, L N; Lam, A; Shelton, C R
2011-06-01
Relative house fly, Musca domestica L., activity at three large dairies in central California was monitored during the peak fly activity period from June to August 2005 by using spot cards, fly tapes, bait traps, and Alsynite traps. Counts for all monitoring methods were significantly related at two of three dairies; with spot card counts significantly related to fly tape counts recorded the same week, and both spot card counts and fly tape counts significantly related to bait trap counts 1-2 wk later. Mean fly counts differed significantly between dairies, but a significant interaction between dairies sampled and monitoring methods used demonstrates that between-dairy comparisons are unwise. Estimate precision was determined by the coefficient of variability (CV) (or SE/mean). Using a CV = 0.15 as a desired level of estimate precision and assuming an integrate pest management (IPM) action threshold near the peak house fly activity measured by each monitoring method, house fly monitoring at a large dairy would require 12 spot cards placed in midafternoon shaded fly resting sites near cattle or seven bait traps placed in open areas near cattle. Software (FlySpotter; http://ucanr.org/ sites/FlySpotter/download/) using computer vision technology was developed to count fly spots on a scanned image of a spot card to dramatically reduce time invested in monitoring house flies. Counts provided by the FlySpotter software were highly correlated to visual counts. The use of spot cards for monitoring house flies is recommended for dairy IPM programs.
ERIC Educational Resources Information Center
Pfeiffer, Mark G.; Scott, Paul G.
A fly-only group (N=16) of Navy replacement pilots undergoing fleet readiness training in the SH-3 helicopter was compared with groups pre-trained on Device 2F64C with: (1) visual only (N=13); (2) no visual/no motion (N=14); and (3) one visual plus motion group (N=19). Groups were compared for their SH-3 helicopter performance in the transition…
Bird Radar Validation in the Field by Time-Referencing Line-Transect Surveys
Dokter, Adriaan M.; Baptist, Martin J.; Ens, Bruno J.; Krijgsveld, Karen L.; van Loon, E. Emiel
2013-01-01
Track-while-scan bird radars are widely used in ornithological studies, but often the precise detection capabilities of these systems are unknown. Quantification of radar performance is essential to avoid observational biases, which requires practical methods for validating a radar’s detection capability in specific field settings. In this study a method to quantify the detection capability of a bird radar is presented, as well a demonstration of this method in a case study. By time-referencing line-transect surveys, visually identified birds were automatically linked to individual tracks using their transect crossing time. Detection probabilities were determined as the fraction of the total set of visual observations that could be linked to radar tracks. To avoid ambiguities in assigning radar tracks to visual observations, the observer’s accuracy in determining a bird’s transect crossing time was taken into account. The accuracy was determined by examining the effect of a time lag applied to the visual observations on the number of matches found with radar tracks. Effects of flight altitude, distance, surface substrate and species size on the detection probability by the radar were quantified in a marine intertidal study area. Detection probability varied strongly with all these factors, as well as species-specific flight behaviour. The effective detection range for single birds flying at low altitude for an X-band marine radar based system was estimated at ∼1.5 km. Within this range the fraction of individual flying birds that were detected by the radar was 0.50±0.06 with a detection bias towards higher flight altitudes, larger birds and high tide situations. Besides radar validation, which we consider essential when quantification of bird numbers is important, our method of linking radar tracks to ground-truthed field observations can facilitate species-specific studies using surveillance radars. The methodology may prove equally useful for optimising tracking algorithms. PMID:24066103
Bird radar validation in the field by time-referencing line-transect surveys.
Dokter, Adriaan M; Baptist, Martin J; Ens, Bruno J; Krijgsveld, Karen L; van Loon, E Emiel
2013-01-01
Track-while-scan bird radars are widely used in ornithological studies, but often the precise detection capabilities of these systems are unknown. Quantification of radar performance is essential to avoid observational biases, which requires practical methods for validating a radar's detection capability in specific field settings. In this study a method to quantify the detection capability of a bird radar is presented, as well a demonstration of this method in a case study. By time-referencing line-transect surveys, visually identified birds were automatically linked to individual tracks using their transect crossing time. Detection probabilities were determined as the fraction of the total set of visual observations that could be linked to radar tracks. To avoid ambiguities in assigning radar tracks to visual observations, the observer's accuracy in determining a bird's transect crossing time was taken into account. The accuracy was determined by examining the effect of a time lag applied to the visual observations on the number of matches found with radar tracks. Effects of flight altitude, distance, surface substrate and species size on the detection probability by the radar were quantified in a marine intertidal study area. Detection probability varied strongly with all these factors, as well as species-specific flight behaviour. The effective detection range for single birds flying at low altitude for an X-band marine radar based system was estimated at ~1.5 km. Within this range the fraction of individual flying birds that were detected by the radar was 0.50 ± 0.06 with a detection bias towards higher flight altitudes, larger birds and high tide situations. Besides radar validation, which we consider essential when quantification of bird numbers is important, our method of linking radar tracks to ground-truthed field observations can facilitate species-specific studies using surveillance radars. The methodology may prove equally useful for optimising tracking algorithms.
Surgical intervention in central toxic keratopathy.
Tu, Kyaw L; Aslanides, Ioannis M
2012-05-03
Purpose. To report management and outcome of 3 cases of bilateral central toxic keratopathy (CTK). Methods. A retrospective chart review on 3 laser-assisted in situ keratomileusis patients who developed CTK within a short time of one another. Results. Patient A had flap lifts and irrigation (FL+I) twice in the right eye (OD) on postoperative day 1 at diffuse lamellar keratitis (DLK) stage 3 and once each on days 1 (at DLK stage 3) and 5 (at CTK) for the left eye (OS). She attained 20/20 unaided visual acuity (UVA) OD at 1 month. Her UVA OS remained at 20/32 but best-corrected visual acuity (BCVA) gradually improved to 20/25 at 8 months. Patient B had right FL+I on day 3 and left FL+I on day 5 (both for CTK). His OS achieved full visual potential (20/25 UVA) by 1 month but UVA OD was reduced to 20/25 (preoperative BCVA 20/20) at 8 months. Patient C had medical management only. Her preoperative BCVA OD of 20/33 fell to 20/50 postoperative UCVA/BCVA; OS regained full visual potential of 20/40 between 2 and 8 months. Patient A's OD did not develop a full-blown CTK; instead an arrested CTK resulted. All except that one eye had initial hyperopic/astigmatic errors that gradually lessened. Artemis II imaging confirmed early stromal loss posterior to the flap with stroma regaining some thickness over the following months. Conclusions. Surgical intervention in cases of CTK may improve clinical outcomes.
Santer, Roger D.
2015-01-01
Tsetse flies transmit trypanosomes that cause nagana in cattle, and sleeping sickness in humans. Therefore, optimising visual baits to control tsetse is an important priority. Tsetse are intercepted at visual baits due to their initial attraction to the bait, and their subsequent contact with it due to landing or accidental collision. Attraction is proposed to be driven in part by a chromatic mechanism to which a UV-blue photoreceptor contributes positively, and a UV and a green photoreceptor contribute negatively. Landing responses are elicited by stimuli with low luminance, but many studies also find apparently strong landing responses when stimuli have high UV reflectivity, which would imply that UV wavelengths contribute negatively to attraction at a distance, but positively to landing responses at close range. The strength of landing responses is often judged using the number of tsetse sampled at a cloth panel expressed as a proportion of the combined catch of the cloth panel and a flanking net that samples circling flies. I modelled these data from two previously published field studies, using calculated fly photoreceptor excitations as predictors. I found that the proportion of tsetse caught on the cloth panel increased with an index representing the chromatic mechanism driving attraction, as would be expected if the same mechanism underlay both long- and close-range attraction. However, the proportion of tsetse caught on the cloth panel also increased with excitation of the UV-sensitive R7p photoreceptor, in an apparently separate but interacting behavioural mechanism. This R7p-driven effect resembles the fly open-space response which is believed to underlie their dispersal towards areas of open sky. As such, the proportion of tsetse that contact a cloth panel likely reflects a combination of deliberate landings by potentially host-seeking tsetse, and accidental collisions by those seeking to disperse, with a separate visual mechanism underlying each behaviour. PMID:26474406
Santer, Roger D
2017-03-01
Riverine tsetse transmit the parasites that cause the most prevalent form of human African trypanosomiasis, Gambian HAT. In response to the imperative for cheap and efficient tsetse control, insecticide-treated 'tiny targets' have been developed through refinement of tsetse attractants based on blue fabric panels. However, modern blue polyesters used for this purpose attract many less tsetse than traditional phthalogen blue cottons. Therefore, colour engineering polyesters for improved attractiveness has great potential for tiny target development. Because flies have markedly different photoreceptor spectral sensitivities from humans, and the responses of these photoreceptors provide the inputs to their visually guided behaviours, it is essential that polyester colour engineering be guided by fly photoreceptor-based explanations of tsetse attraction. To this end, tsetse attraction to differently coloured fabrics was recently modelled using the calculated excitations elicited in a generic set of fly photoreceptors as predictors. However, electrophysiological data from tsetse indicate the potential for modified spectral sensitivities versus the generic pattern, and processing of fly photoreceptor responses within segregated achromatic and chromatic channels has long been hypothesised. Thus, I constructed photoreceptor-based models explaining the attraction of G. f. fuscipes to differently coloured tiny targets recorded in a previously published investigation, under differing assumptions about tsetse spectral sensitivities and organisation of visual processing. Models separating photoreceptor responses into achromatic and chromatic channels explained attraction better than earlier models combining weighted photoreceptor responses in a single mechanism, regardless of the spectral sensitivities assumed. However, common principles for fabric colour engineering were evident across the complete set of models examined, and were consistent with earlier work. Tools for the calculation of fly photoreceptor excitations are available with this paper, and the ways in which these and photoreceptor-based models of attraction can provide colorimetric values for the engineering of more-attractively coloured polyester fabrics are discussed.
2017-01-01
Riverine tsetse transmit the parasites that cause the most prevalent form of human African trypanosomiasis, Gambian HAT. In response to the imperative for cheap and efficient tsetse control, insecticide-treated ‘tiny targets’ have been developed through refinement of tsetse attractants based on blue fabric panels. However, modern blue polyesters used for this purpose attract many less tsetse than traditional phthalogen blue cottons. Therefore, colour engineering polyesters for improved attractiveness has great potential for tiny target development. Because flies have markedly different photoreceptor spectral sensitivities from humans, and the responses of these photoreceptors provide the inputs to their visually guided behaviours, it is essential that polyester colour engineering be guided by fly photoreceptor-based explanations of tsetse attraction. To this end, tsetse attraction to differently coloured fabrics was recently modelled using the calculated excitations elicited in a generic set of fly photoreceptors as predictors. However, electrophysiological data from tsetse indicate the potential for modified spectral sensitivities versus the generic pattern, and processing of fly photoreceptor responses within segregated achromatic and chromatic channels has long been hypothesised. Thus, I constructed photoreceptor-based models explaining the attraction of G. f. fuscipes to differently coloured tiny targets recorded in a previously published investigation, under differing assumptions about tsetse spectral sensitivities and organisation of visual processing. Models separating photoreceptor responses into achromatic and chromatic channels explained attraction better than earlier models combining weighted photoreceptor responses in a single mechanism, regardless of the spectral sensitivities assumed. However, common principles for fabric colour engineering were evident across the complete set of models examined, and were consistent with earlier work. Tools for the calculation of fly photoreceptor excitations are available with this paper, and the ways in which these and photoreceptor-based models of attraction can provide colorimetric values for the engineering of more-attractively coloured polyester fabrics are discussed. PMID:28306721
Localized direction selective responses in the dendrites of visual interneurons of the fly
2010-01-01
Background The various tasks of visual systems, including course control, collision avoidance and the detection of small objects, require at the neuronal level the dendritic integration and subsequent processing of many spatially distributed visual motion inputs. While much is known about the pooled output in these systems, as in the medial superior temporal cortex of monkeys or in the lobula plate of the insect visual system, the motion tuning of the elements that provide the input has yet received little attention. In order to visualize the motion tuning of these inputs we examined the dendritic activation patterns of neurons that are selective for the characteristic patterns of wide-field motion, the lobula-plate tangential cells (LPTCs) of the blowfly. These neurons are known to sample direction-selective motion information from large parts of the visual field and combine these signals into axonal and dendro-dendritic outputs. Results Fluorescence imaging of intracellular calcium concentration allowed us to take a direct look at the local dendritic activity and the resulting local preferred directions in LPTC dendrites during activation by wide-field motion in different directions. These 'calcium response fields' resembled a retinotopic dendritic map of local preferred directions in the receptive field, the layout of which is a distinguishing feature of different LPTCs. Conclusions Our study reveals how neurons acquire selectivity for distinct visual motion patterns by dendritic integration of the local inputs with different preferred directions. With their spatial layout of directional responses, the dendrites of the LPTCs we investigated thus served as matched filters for wide-field motion patterns. PMID:20384983
Neural Summation in the Hawkmoth Visual System Extends the Limits of Vision in Dim Light.
Stöckl, Anna Lisa; O'Carroll, David Charles; Warrant, Eric James
2016-03-21
Most of the world's animals are active in dim light and depend on good vision for the tasks of daily life. Many have evolved visual adaptations that permit a performance superior to that of manmade imaging devices [1]. In insects, a major model visual system, nocturnal species show impressive visual abilities ranging from flight control [2, 3], to color discrimination [4, 5], to navigation using visual landmarks [6-8] or dim celestial compass cues [9, 10]. In addition to optical adaptations that improve their sensitivity in dim light [11], neural summation of light in space and time-which enhances the coarser and slower features of the scene at the expense of noisier finer and faster features-has been suggested to improve sensitivity in theoretical [12-14], anatomical [15-17], and behavioral [18-20] studies. How these summation strategies function neurally is, however, presently unknown. Here, we quantified spatial and temporal summation in the motion vision pathway of a nocturnal hawkmoth. We show that spatial and temporal summation combine supralinearly to substantially increase contrast sensitivity and visual information rate over four decades of light intensity, enabling hawkmoths to see at light levels 100 times dimmer than without summation. Our results reveal how visual motion is calculated neurally in dim light and how spatial and temporal summation improve sensitivity while simultaneously maximizing spatial and temporal resolution, thus extending models of insect motion vision derived predominantly from diurnal flies. Moreover, the summation strategies we have revealed may benefit manmade vision systems optimized for variable light levels [21]. Copyright © 2016 Elsevier Ltd. All rights reserved.
UTM Technical Capabilities Level 2 (TLC2) Test at Reno-Stead Airport.
2016-10-06
Test of Unmanned Aircraft Systems Traffic Management (UTM) technical capability Level 2 (TCL2) at Reno-Stead Airport, Nevada. During the test, five drones simultaneously crossed paths, separated by altitude. Two drones flew beyond visual line-of-sight and three flew within line-of-sight of their operators. Precision Hawk pilot launches UAS Lancaster Mark 3, one of 11 vehicles in the UTM TCL2 demonstration that will fly beyond line of sight of the pilot in command in Nevada test.
Comparison of Visually Guided Flight in Insects and Birds.
Altshuler, Douglas L; Srinivasan, Mandyam V
2018-01-01
Over the last half century, work with flies, bees, and moths have revealed a number of visual guidance strategies for controlling different aspects of flight. Some algorithms, such as the use of pattern velocity in forward flight, are employed by all insects studied so far, and are used to control multiple flight tasks such as regulation of speed, measurement of distance, and positioning through narrow passages. Although much attention has been devoted to long-range navigation and homing in birds, until recently, very little was known about how birds control flight in a moment-to-moment fashion. A bird that flies rapidly through dense foliage to land on a branch-as birds often do-engages in a veritable three-dimensional slalom, in which it has to continually dodge branches and leaves, and find, and possibly even plan a collision-free path to the goal in real time. Each mode of flight from take-off to goal could potentially involve a different visual guidance algorithm. Here, we briefly review strategies for visual guidance of flight in insects, synthesize recent work from short-range visual guidance in birds, and offer a general comparison between the two groups of organisms.
Scalable and Interactive Segmentation and Visualization of Neural Processes in EM Datasets
Jeong, Won-Ki; Beyer, Johanna; Hadwiger, Markus; Vazquez, Amelio; Pfister, Hanspeter; Whitaker, Ross T.
2011-01-01
Recent advances in scanning technology provide high resolution EM (Electron Microscopy) datasets that allow neuroscientists to reconstruct complex neural connections in a nervous system. However, due to the enormous size and complexity of the resulting data, segmentation and visualization of neural processes in EM data is usually a difficult and very time-consuming task. In this paper, we present NeuroTrace, a novel EM volume segmentation and visualization system that consists of two parts: a semi-automatic multiphase level set segmentation with 3D tracking for reconstruction of neural processes, and a specialized volume rendering approach for visualization of EM volumes. It employs view-dependent on-demand filtering and evaluation of a local histogram edge metric, as well as on-the-fly interpolation and ray-casting of implicit surfaces for segmented neural structures. Both methods are implemented on the GPU for interactive performance. NeuroTrace is designed to be scalable to large datasets and data-parallel hardware architectures. A comparison of NeuroTrace with a commonly used manual EM segmentation tool shows that our interactive workflow is faster and easier to use for the reconstruction of complex neural processes. PMID:19834227
Cooperative Physics of Fly Swarms: An Emergent Behavior.
1994-12-01
Report, volume 6, pages 161-178, Berlin, 1977. Dahlem Konferenzen. [10] M. F. Land and T.S. Collett. Chasing behaviour of houseflies (Fannia...Flight control and visual control of flight of the free-flying housefly (Musca domestica). Part III. Philosoph. Trans. Royal Soc. London, B(312):581-595...1986. ] C. Wehrhahn. Sex specific differences in the ori- entation behaviour of houseflies . Biol. Cybernetics, 29:237-247, 1978. [20] C
Nzelu, Chukwunonso O; Gomez, Eduardo A; Cáceres, Abraham G; Sakurai, Tatsuya; Martini-Robles, Luiggi; Uezato, Hiroshi; Mimori, Tatsuyuki; Katakura, Ken; Hashiguchi, Yoshihisa; Kato, Hirotomo
2014-04-01
Entomological monitoring of Leishmania infection in leishmaniasis endemic areas offers epidemiologic advantages for predicting the risk and expansion of the disease, as well as evaluation of the effectiveness of control programs. In this study, we developed a highly sensitive loop-mediated isothermal amplification (LAMP) method for the mass screening of sand flies for Leishmania infection based on the 18S rRNA gene. The LAMP technique could detect 0.01 parasites, which was more sensitive than classical PCR. The method was robust and could amplify the target DNA within 1h from a crude sand fly template without DNA purification. Amplicon detection could be accomplished by the newly developed colorimetric malachite green (MG)--mediated naked eye visualization. Pre-addition of MG to the LAMP reaction solution did not inhibit amplification efficiency. The field applicability of the colorimetric MG-based LAMP assay was demonstrated with 397 field-caught samples from the endemic areas of Ecuador and eight positive sand flies were detected. The robustness, superior sensitivity, and ability to produce better visual discriminatory reaction products than existing LAMP fluorescence and turbidity assays indicated the field potential usefulness of this new method for surveillance and epidemiological studies of leishmaniasis in developing countries. Copyright © 2013 Elsevier B.V. All rights reserved.
Research progress on Drosophila visual cognition in China.
Guo, AiKe; Zhang, Ke; Peng, YueQin; Xi, Wang
2010-03-01
Visual cognition, as one of the fundamental aspects of cognitive neuroscience, is generally associated with high-order brain functions in animals and human. Drosophila, as a model organism, shares certain features of visual cognition in common with mammals at the genetic, molecular, cellular, and even higher behavioral levels. From learning and memory to decision making, Drosophila covers a broad spectrum of higher cognitive behaviors beyond what we had expected. Armed with powerful tools of genetic manipulation in Drosophila, an increasing number of studies have been conducted in order to elucidate the neural circuit mechanisms underlying these cognitive behaviors from a genes-brain-behavior perspective. The goal of this review is to integrate the most important studies on visual cognition in Drosophila carried out in mainland China during the last decade into a body of knowledge encompassing both the basic neural operations and circuitry of higher brain function in Drosophila. Here, we consider a series of the higher cognitive behaviors beyond learning and memory, such as visual pattern recognition, feature and context generalization, different feature memory traces, salience-based decision, attention-like behavior, and cross-modal leaning and memory. We discuss the possible general gain-gating mechanism implementing by dopamine - mushroom body circuit in fly's visual cognition. We hope that our brief review on this aspect will inspire further study on visual cognition in flies, or even beyond.
Real-time tracking of objects for a KC-135 microgravity experiment
NASA Technical Reports Server (NTRS)
Littlefield, Mark L.
1994-01-01
The design of a visual tracking system for use on the Extra-Vehicular Activity Helper/Retriever (EVAHR) is discussed. EVAHR is an autonomous robot designed to perform numerous tasks in an orbital microgravity environment. Since the ability to grasp a freely translating and rotating object is vital to the robot's mission, the EVAHR must analyze range image generated by the primary sensor. This allows EVAHR to locate and focus its sensors so that an accurate set of object poses can be determined and a grasp strategy planned. To test the visual tracking system being developed, a mathematical simulation was used to model the space station environment and maintain dynamics on the EVAHR and any other free floating objects. A second phase of the investigation consists of a series of experiments carried out aboard a KC-135 aircraft flying a parabolic trajectory to simulate microgravity.
Catching What We Can't See: Manual Interception of Occluded Fly-Ball Trajectories
Bosco, Gianfranco; Delle Monache, Sergio; Lacquaniti, Francesco
2012-01-01
Control of interceptive actions may involve fine interplay between feedback-based and predictive mechanisms. These processes rely heavily on target motion information available when the target is visible. However, short-term visual memory signals as well as implicit knowledge about the environment may also contribute to elaborate a predictive representation of the target trajectory, especially when visual feedback is partially unavailable because other objects occlude the visual target. To determine how different processes and information sources are integrated in the control of the interceptive action, we manipulated a computer-generated visual environment representing a baseball game. Twenty-four subjects intercepted fly-ball trajectories by moving a mouse cursor and by indicating the interception with a button press. In two separate sessions, fly-ball trajectories were either fully visible or occluded for 750, 1000 or 1250 ms before ball landing. Natural ball motion was perturbed during the descending trajectory with effects of either weightlessness (0 g) or increased gravity (2 g) at times such that, for occluded trajectories, 500 ms of perturbed motion were visible before ball disappearance. To examine the contribution of previous visual experience with the perturbed trajectories to the interception of invisible targets, the order of visible and occluded sessions was permuted among subjects. Under these experimental conditions, we showed that, with fully visible targets, subjects combined servo-control and predictive strategies. Instead, when intercepting occluded targets, subjects relied mostly on predictive mechanisms based, however, on different type of information depending on previous visual experience. In fact, subjects without prior experience of the perturbed trajectories showed interceptive errors consistent with predictive estimates of the ball trajectory based on a-priori knowledge of gravity. Conversely, the interceptive responses of subjects previously exposed to fully visible trajectories were compatible with the fact that implicit knowledge of the perturbed motion was also taken into account for the extrapolation of occluded trajectories. PMID:23166653
Catching what we can't see: manual interception of occluded fly-ball trajectories.
Bosco, Gianfranco; Delle Monache, Sergio; Lacquaniti, Francesco
2012-01-01
Control of interceptive actions may involve fine interplay between feedback-based and predictive mechanisms. These processes rely heavily on target motion information available when the target is visible. However, short-term visual memory signals as well as implicit knowledge about the environment may also contribute to elaborate a predictive representation of the target trajectory, especially when visual feedback is partially unavailable because other objects occlude the visual target. To determine how different processes and information sources are integrated in the control of the interceptive action, we manipulated a computer-generated visual environment representing a baseball game. Twenty-four subjects intercepted fly-ball trajectories by moving a mouse cursor and by indicating the interception with a button press. In two separate sessions, fly-ball trajectories were either fully visible or occluded for 750, 1000 or 1250 ms before ball landing. Natural ball motion was perturbed during the descending trajectory with effects of either weightlessness (0 g) or increased gravity (2 g) at times such that, for occluded trajectories, 500 ms of perturbed motion were visible before ball disappearance. To examine the contribution of previous visual experience with the perturbed trajectories to the interception of invisible targets, the order of visible and occluded sessions was permuted among subjects. Under these experimental conditions, we showed that, with fully visible targets, subjects combined servo-control and predictive strategies. Instead, when intercepting occluded targets, subjects relied mostly on predictive mechanisms based, however, on different type of information depending on previous visual experience. In fact, subjects without prior experience of the perturbed trajectories showed interceptive errors consistent with predictive estimates of the ball trajectory based on a-priori knowledge of gravity. Conversely, the interceptive responses of subjects previously exposed to fully visible trajectories were compatible with the fact that implicit knowledge of the perturbed motion was also taken into account for the extrapolation of occluded trajectories.
How Lovebirds Maneuver Rapidly Using Super-Fast Head Saccades and Image Feature Stabilization
Kress, Daniel; van Bokhorst, Evelien; Lentink, David
2015-01-01
Diurnal flying animals such as birds depend primarily on vision to coordinate their flight path during goal-directed flight tasks. To extract the spatial structure of the surrounding environment, birds are thought to use retinal image motion (optical flow) that is primarily induced by motion of their head. It is unclear what gaze behaviors birds perform to support visuomotor control during rapid maneuvering flight in which they continuously switch between flight modes. To analyze this, we measured the gaze behavior of rapidly turning lovebirds in a goal-directed task: take-off and fly away from a perch, turn on a dime, and fly back and land on the same perch. High-speed flight recordings revealed that rapidly turning lovebirds perform a remarkable stereotypical gaze behavior with peak saccadic head turns up to 2700 degrees per second, as fast as insects, enabled by fast neck muscles. In between saccades, gaze orientation is held constant. By comparing saccade and wingbeat phase, we find that these super-fast saccades are coordinated with the downstroke when the lateral visual field is occluded by the wings. Lovebirds thus maximize visual perception by overlying behaviors that impair vision, which helps coordinate maneuvers. Before the turn, lovebirds keep a high contrast edge in their visual midline. Similarly, before landing, the lovebirds stabilize the center of the perch in their visual midline. The perch on which the birds land swings, like a branch in the wind, and we find that retinal size of the perch is the most parsimonious visual cue to initiate landing. Our observations show that rapidly maneuvering birds use precisely timed stereotypic gaze behaviors consisting of rapid head turns and frontal feature stabilization, which facilitates optical flow based flight control. Similar gaze behaviors have been reported for visually navigating humans. This finding can inspire more effective vision-based autopilots for drones. PMID:26107413
Garcia-Cantero, Juan J.; Brito, Juan P.; Mata, Susana; Bayona, Sofia; Pastor, Luis
2017-01-01
Gaining a better understanding of the human brain continues to be one of the greatest challenges for science, largely because of the overwhelming complexity of the brain and the difficulty of analyzing the features and behavior of dense neural networks. Regarding analysis, 3D visualization has proven to be a useful tool for the evaluation of complex systems. However, the large number of neurons in non-trivial circuits, together with their intricate geometry, makes the visualization of a neuronal scenario an extremely challenging computational problem. Previous work in this area dealt with the generation of 3D polygonal meshes that approximated the cells’ overall anatomy but did not attempt to deal with the extremely high storage and computational cost required to manage a complex scene. This paper presents NeuroTessMesh, a tool specifically designed to cope with many of the problems associated with the visualization of neural circuits that are comprised of large numbers of cells. In addition, this method facilitates the recovery and visualization of the 3D geometry of cells included in databases, such as NeuroMorpho, and provides the tools needed to approximate missing information such as the soma’s morphology. This method takes as its only input the available compact, yet incomplete, morphological tracings of the cells as acquired by neuroscientists. It uses a multiresolution approach that combines an initial, coarse mesh generation with subsequent on-the-fly adaptive mesh refinement stages using tessellation shaders. For the coarse mesh generation, a novel approach, based on the Finite Element Method, allows approximation of the 3D shape of the soma from its incomplete description. Subsequently, the adaptive refinement process performed in the graphic card generates meshes that provide good visual quality geometries at a reasonable computational cost, both in terms of memory and rendering time. All the described techniques have been integrated into NeuroTessMesh, available to the scientific community, to generate, visualize, and save the adaptive resolution meshes. PMID:28690511
Tactical Versus Strategic Behavior: General Aviation Piloting in Convective Weather Scenarios
NASA Technical Reports Server (NTRS)
Latorella, Kara A.; Chamberlain, James P.
2002-01-01
We commonly describe environments and behavioral responses to environmental conditions as 'tactical' and 'strategic.' However theoretical research defining relevant environmental characteristics is rare, as are empirical investigations that would inform such theory. This paper discusses General Aviation (GA) pilots' descriptions of tactical/strategic conditions with respect to weather flying, and evaluates their ratings along a tactical/strategic scale in response to real convective weather scenarios experienced during a flight experiment with different weather information cues. Perceived risk was significantly associated with ratings for all experimental conditions. In addition, environmental characteristics were found to be predictive of ratings for Traditional IMC (instrument meteorological conditions), i.e., aural weather information only, and Traditional VMC (visual meteorological conditions), i.e., aural information and an external view. The paper also presents subjects' comments regarding use of Graphical Weather Information Systems (GWISs) to support tactical and strategic weather flying decisions and concludes with implications for the design and use of GWISs.
MacKay, Donald G; James, Lori E
2009-10-01
Two experiments compared the visual cognition performance of amnesic H.M. and memory-normal controls matched for age, background, intelligence, and education. In Experiment 1 H.M. exhibited deficits relative to the controls in detecting "erroneous objects" in complex visual scenes--for example, a bird flying inside a fishbowl. In Experiment 2 H.M. exhibited deficits relative to the controls in standard Hidden-Figure tasks when detecting unfamiliar targets but not when detecting familiar targets--for example, circles, squares, and right-angle triangles. H.M.'s visual cognition deficits were not due to his well-known problems in explicit learning and recall, inability to comprehend or remember the instructions, general slowness, motoric difficulties, low motivation, low IQ relative to the controls, or working-memory limitations. Parallels between H.M.'s selective deficits in visual cognition, language, and memory are discussed. These parallels contradict the standard "systems theory" account of H.M.'s condition but comport with the hypothesis that H.M. has difficulty representing unfamiliar but not familiar information in visual cognition, language, and memory. Implications of our results are discussed for binding theory and the ongoing debate over what counts as "memory" versus "not-memory."
Kohatsu, Soh; Yamamoto, Daisuke
2015-03-06
The courtship ritual of male Drosophila represents an innate behaviour that is initiated by female-derived sensory stimuli. Here we report that moving light spots can induce courtship-like following pursuit in tethered wild-type male flies provided the fly is primed by optogenetic stimulation of specific dsx-expressing neuronal clusters in the lateral protocerebrum (LPR). Namely, stimulation of the pC1 neuronal cluster initiates unilateral wing extension and vibration of both sides, whereas stimulation of the pC2l cluster initiates only contralateral wing displays. In addition, stimulation of pC2l but not pC1 neurons induced abdominal bending and proboscis extension. Ca(2+) imaging of the pC1 cluster revealed periodic Ca(2+) rises, each corresponding to a turn of the male fly during courtship. In contrast, group-reared fru mutant males exhibit light spot-induced courtship pursuit without optogenetic priming. Ca(2+) imaging revealed enhanced responses of LPR neurons to visual stimuli in the mutants, suggesting a neural correlate of the light spot-induced courtship behaviour.
[Traumatic lesion of the optic nerve head by flying fish: a case report].
Martin, M; Orgül, S; Robertson, A; Flammer, J
2004-05-01
Traumatic lesion to the optic nerve often leads to severe and persistent functional loss. A male patient was transferred to our hospital from the University Eye Clinic of Guadeloupe 5 days after ocular injury caused by a flying fish. Visual function was light perception. The anterior part of the eye and retina were unremarkable. A computer tomography disclosed a fracture of the sphenoid sinus, with a little bone fragment (DD: foreign body) located close to the optic nerve. Therapy had been started with Aminopenicillin combined with clavulan acid (Augmentin) i. v., 500 ml methylprednisolone (Solumedrol) i. v., lysine-acetyl salicylate (Aspegic) and topical application of dexamethasone combined with neomycin/polymyxin B (Maxitrol). We continued this therapy and intensified it by adding nimodipine (Nimotop) 30 1-1-1 and acetazolamide retard (Diamox sustet) 1-0-1. Unfortunately visual function did not recover under therapy. Traumatic lesions of the optic nerve head, especially when due to axial or tangential forces, can lead to severe and irreversible functional loss. Severe traumatic lesions, even bone fractures induced by flying fish are not a seldom encounter in the Caribbean Sea.
NASA Technical Reports Server (NTRS)
Raney, David L.; Jackson, E. Bruce; Buttrill, Carey S.
2002-01-01
A piloted simulation study conducted in NASA Langley Visual Motion Simulator addressed the impact of dynamic aero- servoelastic effects on flying qualities of a High Speed Civil Transport. The intent was to determine effectiveness of measures to reduce the impact of aircraft flexibility on piloting tasks. Potential solutions examined were increasing frequency of elastic modes through structural stiffening, increasing damping of elastic modes through active control, elimination of control effector excitation of the lowest frequency elastic modes, and elimination of visual cues associated with elastic modes. Six test pilots evaluated and performed simulated maneuver tasks, encountering incidents wherein cockpit vibrations due to elastic modes fed back into the control stick through involuntary vibrations of the pilots upper body and arm. Structural stiffening and compensation of the visual display were of little benefit in alleviating this impact, while increased damping and elimination of control effector excitation of the elastic modes both offered great improvements when applied in sufficient degree.
Advances in Engineering the Fly Genome with the CRISPR-Cas System
Bier, Ethan; Harrison, Melissa M.; O’Connor-Giles, Kate M.; Wildonger, Jill
2018-01-01
Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate relationships between genotype and phenotype, to delineate and visualize how the genome is organized, to illuminate and manipulate RNA, and to pioneer new gene drive technologies. Myriad transformative approaches have already originated from the CRISPR-Cas system, which will likely continue to spark the creation of tools with diverse applications. Here, we provide an overview of how CRISPR-Cas gene editing has revolutionized genetic analysis in Drosophila and highlight key areas for future advances. PMID:29301946
Backscatter absorption gas imaging system
McRae, Jr., Thomas G.
1985-01-01
A video imaging system for detecting hazardous gas leaks. Visual displays of invisible gas clouds are produced by radiation augmentation of the field of view of an imaging device by radiation corresponding to an absorption line of the gas to be detected. The field of view of an imager is irradiated by a laser. The imager receives both backscattered laser light and background radiation. When a detectable gas is present, the backscattered laser light is highly attenuated, producing a region of contrast or shadow on the image. A flying spot imaging system is utilized to synchronously irradiate and scan the area to lower laser power requirements. The imager signal is processed to produce a video display.
Backscatter absorption gas imaging system
McRae, T.G. Jr.
A video imaging system for detecting hazardous gas leaks. Visual displays of invisible gas clouds are produced by radiation augmentation of the field of view of an imaging device by radiation corresponding to an absorption line of the gas to be detected. The field of view of an imager is irradiated by a laser. The imager receives both backscattered laser light and background radiation. When a detectable gas is present, the backscattered laser light is highly attenuated, producing a region of contrast or shadow on the image. A flying spot imaging system is utilized to synchronously irradiate and scan the area to lower laser power requirements. The imager signal is processed to produce a video display.
Stochastic, adaptive sampling of information by microvilli in fly photoreceptors.
Song, Zhuoyi; Postma, Marten; Billings, Stephen A; Coca, Daniel; Hardie, Roger C; Juusola, Mikko
2012-08-07
In fly photoreceptors, light is focused onto a photosensitive waveguide, the rhabdomere, consisting of tens of thousands of microvilli. Each microvillus is capable of generating elementary responses, quantum bumps, in response to single photons using a stochastically operating phototransduction cascade. Whereas much is known about the cascade reactions, less is known about how the concerted action of the microvilli population encodes light changes into neural information and how the ultrastructure and biochemical machinery of photoreceptors of flies and other insects evolved in relation to the information sampling and processing they perform. We generated biophysically realistic fly photoreceptor models, which accurately simulate the encoding of visual information. By comparing stochastic simulations with single cell recordings from Drosophila photoreceptors, we show how adaptive sampling by 30,000 microvilli captures the temporal structure of natural contrast changes. Following each bump, individual microvilli are rendered briefly (~100-200 ms) refractory, thereby reducing quantum efficiency with increasing intensity. The refractory period opposes saturation, dynamically and stochastically adjusting availability of microvilli (bump production rate: sample rate), whereas intracellular calcium and voltage adapt bump amplitude and waveform (sample size). These adapting sampling principles result in robust encoding of natural light changes, which both approximates perceptual contrast constancy and enhances novel events under different light conditions, and predict information processing across a range of species with different visual ecologies. These results clarify why fly photoreceptors are structured the way they are and function as they do, linking sensory information to sensory evolution and revealing benefits of stochasticity for neural information processing. Copyright © 2012 Elsevier Ltd. All rights reserved.
Local Versus Global Effects of Isoflurane Anesthesia on Visual Processing in the Fly Brain
2016-01-01
Abstract What characteristics of neural activity distinguish the awake and anesthetized brain? Drugs such as isoflurane abolish behavioral responsiveness in all animals, implying evolutionarily conserved mechanisms. However, it is unclear whether this conservation is reflected at the level of neural activity. Studies in humans have shown that anesthesia is characterized by spatially distinct spectral and coherence signatures that have also been implicated in the global impairment of cortical communication. We questioned whether anesthesia has similar effects on global and local neural processing in one of the smallest brains, that of the fruit fly (Drosophila melanogaster). Using a recently developed multielectrode technique, we recorded local field potentials from different areas of the fly brain simultaneously, while manipulating the concentration of isoflurane. Flickering visual stimuli (‘frequency tags’) allowed us to track evoked responses in the frequency domain and measure the effects of isoflurane throughout the brain. We found that isoflurane reduced power and coherence at the tagging frequency (13 or 17 Hz) in central brain regions. Unexpectedly, isoflurane increased power and coherence at twice the tag frequency (26 or 34 Hz) in the optic lobes of the fly, but only for specific stimulus configurations. By modeling the periodic responses, we show that the increase in power in peripheral areas can be attributed to local neuroanatomy. We further show that the effects on coherence can be explained by impacted signal-to-noise ratios. Together, our results show that general anesthesia has distinct local and global effects on neuronal processing in the fruit fly brain. PMID:27517084
Local Versus Global Effects of Isoflurane Anesthesia on Visual Processing in the Fly Brain.
Cohen, Dror; Zalucki, Oressia H; van Swinderen, Bruno; Tsuchiya, Naotsugu
2016-01-01
What characteristics of neural activity distinguish the awake and anesthetized brain? Drugs such as isoflurane abolish behavioral responsiveness in all animals, implying evolutionarily conserved mechanisms. However, it is unclear whether this conservation is reflected at the level of neural activity. Studies in humans have shown that anesthesia is characterized by spatially distinct spectral and coherence signatures that have also been implicated in the global impairment of cortical communication. We questioned whether anesthesia has similar effects on global and local neural processing in one of the smallest brains, that of the fruit fly (Drosophila melanogaster). Using a recently developed multielectrode technique, we recorded local field potentials from different areas of the fly brain simultaneously, while manipulating the concentration of isoflurane. Flickering visual stimuli ('frequency tags') allowed us to track evoked responses in the frequency domain and measure the effects of isoflurane throughout the brain. We found that isoflurane reduced power and coherence at the tagging frequency (13 or 17 Hz) in central brain regions. Unexpectedly, isoflurane increased power and coherence at twice the tag frequency (26 or 34 Hz) in the optic lobes of the fly, but only for specific stimulus configurations. By modeling the periodic responses, we show that the increase in power in peripheral areas can be attributed to local neuroanatomy. We further show that the effects on coherence can be explained by impacted signal-to-noise ratios. Together, our results show that general anesthesia has distinct local and global effects on neuronal processing in the fruit fly brain.
Paulk, Angelique C.; Zhou, Yanqiong; Stratton, Peter; Liu, Li
2013-01-01
Neural networks in vertebrates exhibit endogenous oscillations that have been associated with functions ranging from sensory processing to locomotion. It remains unclear whether oscillations may play a similar role in the insect brain. We describe a novel “whole brain” readout for Drosophila melanogaster using a simple multichannel recording preparation to study electrical activity across the brain of flies exposed to different sensory stimuli. We recorded local field potential (LFP) activity from >2,000 registered recording sites across the fly brain in >200 wild-type and transgenic animals to uncover specific LFP frequency bands that correlate with: 1) brain region; 2) sensory modality (olfactory, visual, or mechanosensory); and 3) activity in specific neural circuits. We found endogenous and stimulus-specific oscillations throughout the fly brain. Central (higher-order) brain regions exhibited sensory modality-specific increases in power within narrow frequency bands. Conversely, in sensory brain regions such as the optic or antennal lobes, LFP coherence, rather than power, best defined sensory responses across modalities. By transiently activating specific circuits via expression of TrpA1, we found that several circuits in the fly brain modulate LFP power and coherence across brain regions and frequency domains. However, activation of a neuromodulatory octopaminergic circuit specifically increased neuronal coherence in the optic lobes during visual stimulation while decreasing coherence in central brain regions. Our multichannel recording and brain registration approach provides an effective way to track activity simultaneously across the fly brain in vivo, allowing investigation of functional roles for oscillations in processing sensory stimuli and modulating behavior. PMID:23864378
Stochastic, Adaptive Sampling of Information by Microvilli in Fly Photoreceptors
Song, Zhuoyi; Postma, Marten; Billings, Stephen A.; Coca, Daniel; Hardie, Roger C.; Juusola, Mikko
2012-01-01
Summary Background In fly photoreceptors, light is focused onto a photosensitive waveguide, the rhabdomere, consisting of tens of thousands of microvilli. Each microvillus is capable of generating elementary responses, quantum bumps, in response to single photons using a stochastically operating phototransduction cascade. Whereas much is known about the cascade reactions, less is known about how the concerted action of the microvilli population encodes light changes into neural information and how the ultrastructure and biochemical machinery of photoreceptors of flies and other insects evolved in relation to the information sampling and processing they perform. Results We generated biophysically realistic fly photoreceptor models, which accurately simulate the encoding of visual information. By comparing stochastic simulations with single cell recordings from Drosophila photoreceptors, we show how adaptive sampling by 30,000 microvilli captures the temporal structure of natural contrast changes. Following each bump, individual microvilli are rendered briefly (∼100–200 ms) refractory, thereby reducing quantum efficiency with increasing intensity. The refractory period opposes saturation, dynamically and stochastically adjusting availability of microvilli (bump production rate: sample rate), whereas intracellular calcium and voltage adapt bump amplitude and waveform (sample size). These adapting sampling principles result in robust encoding of natural light changes, which both approximates perceptual contrast constancy and enhances novel events under different light conditions, and predict information processing across a range of species with different visual ecologies. Conclusions These results clarify why fly photoreceptors are structured the way they are and function as they do, linking sensory information to sensory evolution and revealing benefits of stochasticity for neural information processing. PMID:22704990
Discriminating External and Internal Causes for Heading Changes in Freely Flying Drosophila
Sayaman, Rosalyn W.; Murray, Richard M.; Dickinson, Michael H.
2013-01-01
As animals move through the world in search of resources, they change course in reaction to both external sensory cues and internally-generated programs. Elucidating the functional logic of complex search algorithms is challenging because the observable actions of the animal cannot be unambiguously assigned to externally- or internally-triggered events. We present a technique that addresses this challenge by assessing quantitatively the contribution of external stimuli and internal processes. We apply this technique to the analysis of rapid turns (“saccades”) of freely flying Drosophila melanogaster. We show that a single scalar feature computed from the visual stimulus experienced by the animal is sufficient to explain a majority (93%) of the turning decisions. We automatically estimate this scalar value from the observable trajectory, without any assumption regarding the sensory processing. A posteriori, we show that the estimated feature field is consistent with previous results measured in other experimental conditions. The remaining turning decisions, not explained by this feature of the visual input, may be attributed to a combination of deterministic processes based on unobservable internal states and purely stochastic behavior. We cannot distinguish these contributions using external observations alone, but we are able to provide a quantitative bound of their relative importance with respect to stimulus-triggered decisions. Our results suggest that comparatively few saccades in free-flying conditions are a result of an intrinsic spontaneous process, contrary to previous suggestions. We discuss how this technique could be generalized for use in other systems and employed as a tool for classifying effects into sensory, decision, and motor categories when used to analyze data from genetic behavioral screens. PMID:23468601
NASA Astrophysics Data System (ADS)
Kachejian, Kerry C.; Vujcic, Doug
1999-07-01
The Tactical Visualization Module (TVM) research effort will develop and demonstrate a portable, tactical information system to enhance the situational awareness of individual warfighters and small military units by providing real-time access to manned and unmanned aircraft, tactically mobile robots, and unattended sensors. TVM consists of a family of portable and hand-held devices being advanced into a next- generation, embedded capability. It enables warfighters to visualize the tactical situation by providing real-time video, imagery, maps, floor plans, and 'fly-through' video on demand. When combined with unattended ground sensors, such as Combat- Q, TVM permits warfighters to validate and verify tactical targets. The use of TVM results in faster target engagement times, increased survivability, and reduction of the potential for fratricide. TVM technology can support both mounted and dismounted tactical forces involved in land, sea, and air warfighting operations. As a PCMCIA card, TVM can be embedded in portable, hand-held, and wearable PCs. Thus, it leverages emerging tactical displays including flat-panel, head-mounted displays. The end result of the program will be the demonstration of the system with U.S. Army and USMC personnel in an operational environment. Raytheon Systems Company, the U.S. Army Soldier Systems Command -- Natick RDE Center (SSCOM- NRDEC) and the Defense Advanced Research Projects Agency (DARPA) are partners in developing and demonstrating the TVM technology.
Bio-inspired motion detection in an FPGA-based smart camera module.
Köhler, T; Röchter, F; Lindemann, J P; Möller, R
2009-03-01
Flying insects, despite their relatively coarse vision and tiny nervous system, are capable of carrying out elegant and fast aerial manoeuvres. Studies of the fly visual system have shown that this is accomplished by the integration of signals from a large number of elementary motion detectors (EMDs) in just a few global flow detector cells. We developed an FPGA-based smart camera module with more than 10,000 single EMDs, which is closely modelled after insect motion-detection circuits with respect to overall architecture, resolution and inter-receptor spacing. Input to the EMD array is provided by a CMOS camera with a high frame rate. Designed as an adaptable solution for different engineering applications and as a testbed for biological models, the EMD detector type and parameters such as the EMD time constants, the motion-detection directions and the angle between correlated receptors are reconfigurable online. This allows a flexible and simultaneous detection of complex motion fields such as translation, rotation and looming, such that various tasks, e.g., obstacle avoidance, height/distance control or speed regulation can be performed by the same compact device.
UTM Technical Capabilities Level 2 (TLC2) Test at Reno-Stead Airport.
2016-10-06
Test of Unmanned Aircraft Systems Traffic Management (UTM) technical capability Level 2 (TCL2) at Reno-Stead Airport, Nevada. During the test, five drones simultaneously crossed paths, separated by altitude. Two drones flew beyond visual line-of-sight and three flew within line-of-sight of their operators. Drone Co-habitation Services operates a Phantom 3 commercial multi-rotor unmanned aircraft, one of 11 vehicles in the UTM TCL2 demonstration that will fly beyond line of sight of the pilot in command in Nevada test.
NASA Technical Reports Server (NTRS)
De La Cruz, Melinda; Henderson, Steve
2016-01-01
The RAPTR was developed to test ISS payloads for NASA. RAPTR is a simulation of the Command and Data Handling (C&DH) interfaces of the ISS (MIL-STD1553B, Ethernet and TAXI) and is designed for rapid testing and deployment of payload experiments to the ISS. The ISS's goal is to reduce the amount of time it takes for a payload developer to build, test and fly a payload, including payload software. The RAPTR meets this need with its user oriented, visually rich interface.
Astronauts Cooper and Conrad prepare cameras during visual acuity tests
NASA Technical Reports Server (NTRS)
1965-01-01
Astronauts L. Gordon Cooper Jr. (left), command pilot, and Charles Conrad Jr., pilot, the prime crew of the Gemini 5 space flight, prepare their cameras while aboard a C-130 aircraft flying near Laredo. The two astronauts are taking part in a series of visual acuity experiments to aid them in learning to identify known terrestrial features under controlled conditions.
Images of Earth and Space: The Role of Visualization in NASA Science
NASA Technical Reports Server (NTRS)
1996-01-01
Fly through the ocean at breakneck speed. Tour the moon. Even swim safely in the boiling sun. You can do these things and more in a 17 minute virtual journey through Earth and space. The trek is by way of colorful scientific visualizations developed by the NASA/Goddard Space Flight Center's Scientific Visualization Studio and the NASA HPCC Earth and Space Science Project investigators. Various styles of electronic music and lay-level narration provide the accompaniment.
Jung, Kwang Bo; Lee, Hana; Son, Ye Seul; Lee, Ji Hye; Cho, Hyun-Soo; Lee, Mi-Ok; Oh, Jung-Hwa; Lee, Jaemin; Kim, Seokho; Jung, Cho-Rok; Kim, Janghwan; Son, Mi-Young
2018-01-01
Human intestinal organoids (hIOs) derived from human pluripotent stem cells (hPSCs) have immense potential as a source of intestines. Therefore, an efficient system is needed for visualizing the stage of intestinal differentiation and further identifying hIOs derived from hPSCs. Here, 2 fluorescent biosensors were developed based on human induced pluripotent stem cell (hiPSC) lines that stably expressed fluorescent reporters driven by intestine-specific gene promoters Krüppel-like factor 5 monomeric Cherry (KLF5 mCherry ) and intestine-specific homeobox enhanced green fluorescence protein (ISX eGFP ). Then hIOs were efficiently induced from those transgenic hiPSC lines in which mCherry- or eGFP-expressing cells, which appeared during differentiation, could be identified in intact living cells in real time. Reporter gene expression had no adverse effects on differentiation into hIOs and proliferation. Using our reporter system to screen for hIO differentiation factors, we identified DMH1 as an efficient substitute for Noggin. Transplanted hIOs under the kidney capsule were tracked with fluorescence imaging (FLI) and confirmed histologically. After orthotopic transplantation, the localization of the hIOs in the small intestine could be accurately visualized using FLI. Our study establishes a selective system for monitoring the in vitro differentiation and for tracking the in vivo localization of hIOs and contributes to further improvement of cell-based therapies and preclinical screenings in the intestinal field.-Jung, K. B., Lee, H., Son, Y. S., Lee, J. H., Cho, H.-S., Lee, M.-O., Oh, J.-H., Lee, J., Kim, S., Jung, C.-R., Kim, J., Son, M.-Y. In vitro and in vivo imaging and tracking of intestinal organoids from human induced pluripotent stem cells. © FASEB.
Brewer, Michael J; Noma, Takuji
2010-06-01
We integrated a natural enemy survey of the broader landscape into a more traditional survey for Aphis glycines Matsumura (Hemiptera: Aphididae), parasitoids and predatory flies on soybean using A. glycines-infested soybean, Glycine max (L.) Merr., placed in cropped and noncropped plant systems to complement visual field observations. Across three sites and 5 yr, 18 parasitoids and predatory flies in total (Hymenoptera: Aphelinidae [two species] and Bracondae [seven species], Diptera: Cecidomyiidae [one species], Syrphidae [seven species], Chamaemyiidae [one species]) were detected, with significant variability in recoveries detected across plant system treatments and strong contrasts in habitat affinity detected among species. Lysiphlebus testaceipes Cresson was the most frequently detected parasitoid, and no differences in abundance were detected in cropped (soybean, wheat [Triticum aestivum L.], corn [Zea mays L.], and alfalfa [Medicago sativa L.]) and noncropped (poplar [Populus euramericana (Dode) Guinier] and early successional vegetation) areas. In contrast, Binodoxys kelloggensis Pike, Starý & Brewer had strong habitat affinity for poplar and early successional vegetation. The low recoveries seasonally and across habitats of Aphelinus asychis Walker, Aphelinus sp., and Aphidius colemoni Viereck make their suitability to A. glycines on soybean highly suspect. The widespread occurrence of many of the flies reflects their broad habitat affinity and host aphid ranges. The consistent low field observations of parasitism and predation suggest that resident parasitoids and predatory flies are unlikely to contribute substantially to A. glycines suppression, at least during the conventional time period early in the pest invasion when classical biological control activities are considered. For selected species that were relatively well represented across plant systems (i.e., L. testaceipes and Aphidoletes aphidimyza Rondani), conservation biological control efforts may be fruitful. The additional information gained from expanding the natural enemy survey into the broader landscape was essential in making these distinctions relevant to conservation biological control, while adding agroecosystem-specific information valuable to classical biological control.
High resolution imaging of a subsonic projectile using automated mirrors with large aperture
NASA Astrophysics Data System (ADS)
Tateno, Y.; Ishii, M.; Oku, H.
2017-02-01
Visual tracking of high-speed projectiles is required for studying the aerodynamics around the objects. One solution to this problem is a tracking method based on the so-called 1 ms Auto Pan-Tilt (1ms-APT) system that we proposed in previous work, which consists of rotational mirrors and a high-speed image processing system. However, the images obtained with that system did not have high enough resolution to realize detailed measurement of the projectiles because of the size of the mirrors. In this study, we propose a new system consisting of enlarged mirrors for tracking a high-speed projectiles so as to achieve higher-resolution imaging, and we confirmed the effectiveness of the system via an experiment in which a projectile flying at subsonic speed tracked.
Kijak, Ewelina; Pyza, Elżbieta
2017-01-01
Drosophila melanogaster is a common model used to study circadian rhythms in behavior and circadian clocks. However, numerous circadian rhythms have also been detected in non-clock neurons, especially in the first optic neuropil (lamina) of the fly's visual system. Such rhythms have been observed in the number of synapses and in the structure of interneurons, which exhibit changes in size and shape in a circadian manner. Although the patterns of these changes are known, the mechanism remains unclear. In the present study, we investigated the role of the TOR signaling pathway and autophagy in regulating circadian rhythms based on the behavior and structural plasticity of the lamina L2 monopolar cell dendritic trees. In addition, we examined the cyclic expression of the TOR signaling pathway (Tor, Pi3K class 1, Akt1) and autophagy (Atg5 and Atg7) genes in the fly's brain. We observed that Tor, Atg5 and Atg7 exhibit rhythmic expressions in the brain of wild-type flies in day/night conditions (LD 12:12) that are abolished in per01 clock mutants. The silencing of Tor in per expressing cells shortens a period of the locomotor activity rhythm of flies. In addition, silencing of the Tor and Atg5 genes in L2 cells disrupts the circadian plasticity of the L2 cell dendritic trees measured in the distal lamina. In turn, silencing of the Atg7 gene in L2 cells changes the pattern of this rhythm. Our results indicate that the TOR signaling pathway and autophagy are involved in the regulation of circadian rhythms in the behavior and plasticity of neurons in the brain of adult flies.
Lonely Skies: Air-to-Air Training for a 5th Generation Fighter Force
2015-06-01
Missing Attitude Indicator….…………………14 4 Lt James Doolittle during Blind Flight Test…..……………………...15 5 An Early Link Trainer Cockpit...during visual flight because it deceived pilots about the actual aircraft attitude and acceleration. 1st Lt James Doolittle used Doctor David Meyers...flying pioneer and leader, Doolittle believed that pilots should learn to ignore their physical sense of motion while flying blind and to trust their
Flies lacking all synapsins are unexpectedly healthy but are impaired in complex behaviour.
Godenschwege, Tanja A; Reisch, Dietmar; Diegelmann, Sören; Eberle, Kai; Funk, Natalja; Heisenberg, Martin; Hoppe, Viviane; Hoppe, Jürgen; Klagges, Bert R E; Martin, Jean-René; Nikitina, Ekaterina A; Putz, Gabi; Reifegerste, Rita; Reisch, Natascha; Rister, Jens; Schaupp, Michael; Scholz, Henrike; Schwärzel, Martin; Werner, Ursula; Zars, Troy D; Buchner, Sigrid; Buchner, Erich
2004-08-01
Vertebrate synapsins are abundant synaptic vesicle phosphoproteins that have been proposed to fine-regulate neurotransmitter release by phosphorylation-dependent control of synaptic vesicle motility. However, the consequences of a total lack of all synapsin isoforms due to a knock-out of all three mouse synapsin genes have not yet been investigated. In Drosophila a single synapsin gene encodes several isoforms and is expressed in most synaptic terminals. Thus the targeted deletion of the synapsin gene of Drosophila eliminates the possibility of functional knock-out complementation by other isoforms. Unexpectedly, synapsin null mutant flies show no obvious defects in brain morphology, and no striking qualitative changes in behaviour are observed. Ultrastructural analysis of an identified 'model' synapse of the larval nerve muscle preparation revealed no difference between wild-type and mutant, and spontaneous or evoked excitatory junction potentials at this synapse were normal up to a stimulus frequency of 5 Hz. However, when several behavioural responses were analysed quantitatively, specific differences between mutant and wild-type flies are noted. Adult locomotor activity, optomotor responses at high pattern velocities, wing beat frequency, and visual pattern preference are modified. Synapsin mutant flies show faster habituation of an olfactory jump response, enhanced ethanol tolerance, and significant defects in learning and memory as measured using three different paradigms. Larval behavioural defects are described in a separate paper. We conclude that Drosophila synapsins play a significant role in nervous system function, which is subtle at the cellular level but manifests itself in complex behaviour.
NASA Technical Reports Server (NTRS)
Bailey, R. E.; Smith, R. E.
1982-01-01
An investigation of pilot-induced oscillation suppression (PIOS) filters was performed using the USAF/Flight Dynamics Laboratory variable stability NT-33 aircraft, modified and operated by Calspan. This program examined the effects of PIOS filtering on the longitudinal flying qualities of fighter aircraft during the visual approach and landing task. Forty evaluations were flown to test the effects of different PIOS filters. Although detailed analyses were not undertaken, the results indicate that PIOS filtering can improve the flying qualities of an otherwise unacceptable aircraft configuration (Level 3 flying qualities). However, the ability of the filters to suppress pilot-induced oscillations appears to be dependent upon the aircraft configuration characteristics. Further, the data show that the filters can adversely affect landing flying qualities if improperly designed. The data provide an excellent foundation from which detail analyses can be performed.
The relationship between morphological and behavioral mimicry in hover flies (Diptera: Syrphidae).
Penney, Heather D; Hassall, Christopher; Skevington, Jeffrey H; Lamborn, Brent; Sherratt, Thomas N
2014-02-01
Palatable (Batesian) mimics of unprofitable models could use behavioral mimicry to compensate for the ease with which they can be visually discriminated or to augment an already close morphological resemblance. We evaluated these contrasting predictions by assaying the behavior of 57 field-caught species of mimetic hover flies (Diptera: Syrphidae) and quantifying their morphological similarity to a range of potential hymenopteran models. A purpose-built phylogeny for the hover flies was used to control for potential lack of independence due to shared evolutionary history. Those hover fly species that engage in behavioral mimicry (mock stinging, leg waving, wing wagging) were all large wasp mimics within the genera Spilomyia and Temnostoma. While the behavioral mimics assayed were good morphological mimics, not all good mimics were behavioral mimics. Therefore, while the behaviors may have evolved to augment good morphological mimicry, they do not advantage all good mimics.
Spatio-temporal visualization of air-sea CO2 flux and carbon budget using volume rendering
NASA Astrophysics Data System (ADS)
Du, Zhenhong; Fang, Lei; Bai, Yan; Zhang, Feng; Liu, Renyi
2015-04-01
This paper presents a novel visualization method to show the spatio-temporal dynamics of carbon sinks and sources, and carbon fluxes in the ocean carbon cycle. The air-sea carbon budget and its process of accumulation are demonstrated in the spatial dimension, while the distribution pattern and variation of CO2 flux are expressed by color changes. In this way, we unite spatial and temporal characteristics of satellite data through visualization. A GPU-based direct volume rendering technique using half-angle slicing is adopted to dynamically visualize the released or absorbed CO2 gas with shadow effects. A data model is designed to generate four-dimensional (4D) data from satellite-derived air-sea CO2 flux products, and an out-of-core scheduling strategy is also proposed for on-the-fly rendering of time series of satellite data. The presented 4D visualization method is implemented on graphics cards with vertex, geometry and fragment shaders. It provides a visually realistic simulation and user interaction for real-time rendering. This approach has been integrated into the Information System of Ocean Satellite Monitoring for Air-sea CO2 Flux (IssCO2) for the research and assessment of air-sea CO2 flux in the China Seas.
Task and postural factors are related to back pain in helicopter pilots.
Bridger, R S; Groom, M R; Jones, H; Pethybridge, R J; Pullinger, N
2002-08-01
A previous survey by Shear et al. revealed a high prevalence of back pain in Royal Navy helicopter aircrew, compared with controls. It was recommended that a second survey be undertaken, taking account of flying tasks and cockpit ergonomics. This was the purpose of the present investigation. A questionnaire containing items on back pain and posture was circulated to all 246 acting pilots, with returns of 75%. The questionnaire sought information on pain in both the flying pilot and co-pilot/instructor roles. The 12-mo prevalence of back pain was 80%. Task-related back pain was greatest in instrument flying (72%) and least in the co-pilot and instructor roles (24%). Self-ratings of posture indicated that forward flexed trunk postures predominated in the flying roles and were most extreme in instrument flying. In non-flying roles, symmetrical, reclining postures were more often reported. No demographic or psychosocial variables were significantly related to back pain prevalence or disability. Much of the back pain experienced by helicopter pilots appears to be due to the posture needed to operate the cyclic and collective controls. In instrument flying, it is suggested that the visual demands of scanning the displays may exacerbate the pain by causing the pilot to lean further forward.
Martín-Vega, Daniel; Simonsen, Thomas J; Hall, Martin J R
2017-05-01
Metamorphosis of cyclorrhaphous flies takes place inside a barrel-like puparium, formed by the shrinking, hardening and darkening of the third-instar larval cuticle. The opacity of this structure hampers the visualization of the morphological changes occurring inside and therefore a full understanding of the metamorphosis process. Here, we use micro-computed tomography (micro-CT) to describe the internal morphological changes that occur during metamorphosis of the blow fly, Calliphora vicina Robineau-Desvoidy 1830 (Diptera: Calliphoridae) at a greater temporal resolution than anything hitherto published. The morphological changes were documented at 10% intervals of the total intra-puparial period, and down to 2.5% intervals during the first 20% interval, when the most dramatic morphological changes occur. Moreover, the development of an internal gas bubble, which plays an essential role during early metamorphosis, was further investigated with X-ray images and micro-CT virtual sections. The origin of this gas bubble has been largely unknown, but micro-CT virtual sections show that it is connected to one of the main tracheal trunks. Micro-CT virtual sections also provided enough resolution for determining the completion of the larval-pupal and pupal-adult apolyses, thus enabling an accurate timing of the different intra-puparial life stages. The prepupal, pupal, and pharate adult stages last for 7.5%, 22.5%, and 70% of the total intra-puparial development, respectively. Furthermore, we provide for the first time quantitative data on the development of two organ systems of the blow fly: the alimentary canal and the indirect flight muscles. There is a significant and negative correlation between the volume of the indirect flight muscles and the pre-helicoidal region of the midgut during metamorphosis. The latter occupies a large portion of the thorax during the pupal stage but narrows progressively as the indirect flight muscles increase in volume during the development of the pharate adult. © 2017 The Authors Journal of Morphology Published by Wiley Periodicals, Inc.
Simonsen, Thomas J.; Hall, Martin J. R.
2017-01-01
Abstract Metamorphosis of cyclorrhaphous flies takes place inside a barrel‐like puparium, formed by the shrinking, hardening and darkening of the third‐instar larval cuticle. The opacity of this structure hampers the visualization of the morphological changes occurring inside and therefore a full understanding of the metamorphosis process. Here, we use micro‐computed tomography (micro‐CT) to describe the internal morphological changes that occur during metamorphosis of the blow fly, Calliphora vicina Robineau‐Desvoidy 1830 (Diptera: Calliphoridae) at a greater temporal resolution than anything hitherto published. The morphological changes were documented at 10% intervals of the total intra‐puparial period, and down to 2.5% intervals during the first 20% interval, when the most dramatic morphological changes occur. Moreover, the development of an internal gas bubble, which plays an essential role during early metamorphosis, was further investigated with X‐ray images and micro‐CT virtual sections. The origin of this gas bubble has been largely unknown, but micro‐CT virtual sections show that it is connected to one of the main tracheal trunks. Micro‐CT virtual sections also provided enough resolution for determining the completion of the larval‐pupal and pupal‐adult apolyses, thus enabling an accurate timing of the different intra‐puparial life stages. The prepupal, pupal, and pharate adult stages last for 7.5%, 22.5%, and 70% of the total intra‐puparial development, respectively. Furthermore, we provide for the first time quantitative data on the development of two organ systems of the blow fly: the alimentary canal and the indirect flight muscles. There is a significant and negative correlation between the volume of the indirect flight muscles and the pre‐helicoidal region of the midgut during metamorphosis. The latter occupies a large portion of the thorax during the pupal stage but narrows progressively as the indirect flight muscles increase in volume during the development of the pharate adult. PMID:28182298
High-Performance Computing Unlocks Innovation at NREL
DOE Office of Scientific and Technical Information (OSTI.GOV)
None
Need to fly around a wind farm? Or step inside a molecule? NREL scientists use a super powerful (and highly energy-efficient) computer to visualize and solve big problems in renewable energy research.
Localization from Visual Landmarks on a Free-Flying Robot
NASA Technical Reports Server (NTRS)
Coltin, Brian; Fusco, Jesse; Moratto, Zack; Alexandrov, Oleg; Nakamura, Robert
2016-01-01
We present the localization approach for Astrobee, a new free-flying robot designed to navigate autonomously on the International Space Station (ISS). Astrobee will accommodate a variety of payloads and enable guest scientists to run experiments in zero-g, as well as assist astronauts and ground controllers. Astrobee will replace the SPHERES robots which currently operate on the ISS, whose use of fixed ultrasonic beacons for localization limits them to work in a 2 meter cube. Astrobee localizes with monocular vision and an IMU, without any environmental modifications. Visual features detected on a pre-built map, optical flow information, and IMU readings are all integrated into an extended Kalman filter (EKF) to estimate the robot pose. We introduce several modifications to the filter to make it more robust to noise, and extensively evaluate the localization algorithm.
Khan, S; Pierce, D; Vale, R D
The chemotaxis signal protein CheY of enteric bacteria shuttles between transmembrane methyl-accepting chemotaxis protein (MCP) receptor complexes and flagellar basal bodies [1]. The basal body C-rings, composed of the FliM, FliG and FliN proteins, form the rotor of the flagellar motor [2]. Phosphorylated CheY binds to isolated FliM [3] and may also interact with FliG [4], but its binding to basal bodies has not been measured. Using the chemorepellent acetate to phosphorylate and acetylate CheY [5], we have measured the covalent-modification-dependent binding of a green fluorescent protein-CheY fusion (GFP-CheY) to motor assemblies in bacteria lacking MCP complexes by evanescent wave microscopy [6]. At acetate concentrations that cause solely clockwise rotation, GFP-CheY molecules bound to native basal bodies or to overproduced rotor complexes with a stoichiometry comparable to the number of C-ring subunits. GFP-CheY did not bind to rotors lacking FIiM/FliN, showing that these subunits are essential for the association. This assay provides a new means of monitoring protein-protein interactions in signal transduction pathways in living cells.
Optical emission spectroscopy of point-plane corona and back-corona discharges in air
NASA Astrophysics Data System (ADS)
Czech, T.; Sobczyk, A. T.; Jaworek, A.
2011-12-01
Results of spectroscopic investigations and current-voltage characteristics of corona discharge and back discharge on fly-ash layer, generated in point-plane electrode geometry in air at atmospheric pressure are presented in the paper. The characteristics of both discharges are similar but differ in the current and voltage ranges of all the discharge forms distinguished during the experiments. Three forms of back discharge, for positive and negative polarity, were investigated: glow, streamer and low-current back-arc. In order to characterize ionisation and excitation processes in back discharge, the emission spectra were measured and compared with those obtained for normal corona discharge generated in the same electrode configuration but with fly ash layer removed. The emission spectra were measured in two discharge zones: near the tip of needle electrode and near the plate. Visual forms of the discharge were recorded with digital camera and referred to current-voltage characteristics and emission spectra. The measurements have shown that spectral lines emitted by back discharge depend on the form of discharge and the discharge current. From the comparison of the spectral lines of back and normal discharges an effect of fly ash layer on the discharge morphology can be determined. The recorded emission spectra formed by ionised gas and plasma near the needle electrode and fly ash layer are different. It should be noted that in back arc emission, spectral lines of fly ash layer components can be distinguished. On the other hand, in needle zone, the emission of high intensity N2 second positive system and NO γ lines can be noticed. Regardless of these gaseous lines, also atomic lines of dust layer were present in the spectrum. The differences in spectra of back discharge for positive and negative polarities of the needle electrode have been explained by considering the kind of ions generated in the crater in fly ash layer. The aim of these studies is to better understand the discharge processes encountered in electrostatic precipitators.
ERIC Educational Resources Information Center
Kuntz, Sara; Poeck, Burkhard; Sokolowski, Marla B.; Strauss, Roland
2012-01-01
Orientation and navigation in a complex environment requires path planning and recall to exert goal-driven behavior. Walking "Drosophila" flies possess a visual orientation memory for attractive targets which is localized in the central complex of the adult brain. Here we show that this type of working memory requires the cGMP-dependent protein…
Flare cue symbology and EVS for zero-zero weather landing
NASA Astrophysics Data System (ADS)
French, Guy A.; Murphy, David M.; Ercoline, William R.
2006-05-01
When flying an airplane, landing is arguably the most difficult task a pilot can do. This applies to pilots of all skill levels particularly as the level of complexity in both the aircraft and environment increase. Current navigational aids, such as an instrument landing system (ILS), do a good job of providing safe guidance for an approach to an airfield. These aids provide data to primary flight reference (PFR) displays on-board the aircraft depicting through symbology what the pilot's eyes should be seeing. Piloting an approach under visual meteorological conditions (VMC) is relatively easy compared to the various complex instrument approaches under instrument meteorological conditions (IMC) which may include flying in zero-zero weather. Perhaps the most critical point in the approach is the transition to landing where the rate of closure between the wheels and the runway is critical to a smooth, accurate landing. Very few PFR's provide this flare cue information. In this study we will evaluate examples of flare cueing symbology for use in landing an aircraft in the most difficult conditions. This research is a part of a larger demonstration effort using sensor technology to land in zero-zero weather at airfields that offer no or unreliable approach guidance. Several problems exist when landing without visual reference to the outside world. One is landing with a force greater than desired at touchdown and another is landing on a point of the runway other than desired. We compare different flare cueing systems to one another and against a baseline for completing this complex approach task.
NASA Astrophysics Data System (ADS)
Fang, H.; Kato, H.; Rodell, M.; Teng, W. L.; Vollmer, B. E.
2008-12-01
The Global Land Data Assimilation System (GLDAS) has been generating a series of land surface state (e.g., soil moisture and surface temperature) and flux (e.g., evaporation and sensible heat flux) products, simulated by four land surface models (CLM, Mosaic, Noah and VIC). These products are now accessible at the Hydrology Data and Information Services Center (HDISC), a component of the NASA Goddard Earth Sciences Data and Information Services Center (GES DISC). Current GLDAS data hosted at HDISC include a set of 1.0° data products, covering 1979 to the present, from the four models and a 0.25° data product, covering 2000 to the present, from the Noah model. In addition to the basic anonymous ftp data downloading, users can avail themselves of several advanced data search and downloading services, such as Mirador and OPeNDAP. Mirador is a Google-based search tool that provides keywords searching, on-the-fly spatial and parameter subsetting of selected data. OPeNDAP (Open-source Project for a Network Data Access Protocol) enables remote OPeNDAP clients to access OPeNDAP served data regardless of local storage format. Additional data services to be available in the near future from HDISC include (1) on-the-fly converter of GLDAS to NetCDF and binary data formats; (2) temporal aggregation of GLDAS files; and (3) Giovanni, an online visualization and analysis tool that provides a simple way to visualize, analyze, and access vast amounts of data without having to download the data.
Optic flow cues guide flight in birds.
Bhagavatula, Partha S; Claudianos, Charles; Ibbotson, Michael R; Srinivasan, Mandyam V
2011-11-08
Although considerable effort has been devoted to investigating how birds migrate over large distances, surprisingly little is known about how they tackle so successfully the moment-to-moment challenges of rapid flight through cluttered environments [1]. It has been suggested that birds detect and avoid obstacles [2] and control landing maneuvers [3-5] by using cues derived from the image motion that is generated in the eyes during flight. Here we investigate the ability of budgerigars to fly through narrow passages in a collision-free manner, by filming their trajectories during flight in a corridor where the walls are decorated with various visual patterns. The results demonstrate, unequivocally and for the first time, that birds negotiate narrow gaps safely by balancing the speeds of image motion that are experienced by the two eyes and that the speed of flight is regulated by monitoring the speed of image motion that is experienced by the two eyes. These findings have close parallels with those previously reported for flying insects [6-13], suggesting that some principles of visual guidance may be shared by all diurnal, flying animals. Copyright © 2011 Elsevier Ltd. All rights reserved.
FlyAR: augmented reality supported micro aerial vehicle navigation.
Zollmann, Stefanie; Hoppe, Christof; Langlotz, Tobias; Reitmayr, Gerhard
2014-04-01
Micro aerial vehicles equipped with high-resolution cameras can be used to create aerial reconstructions of an area of interest. In that context automatic flight path planning and autonomous flying is often applied but so far cannot fully replace the human in the loop, supervising the flight on-site to assure that there are no collisions with obstacles. Unfortunately, this workflow yields several issues, such as the need to mentally transfer the aerial vehicles position between 2D map positions and the physical environment, and the complicated depth perception of objects flying in the distance. Augmented Reality can address these issues by bringing the flight planning process on-site and visualizing the spatial relationship between the planned or current positions of the vehicle and the physical environment. In this paper, we present Augmented Reality supported navigation and flight planning of micro aerial vehicles by augmenting the users view with relevant information for flight planning and live feedback for flight supervision. Furthermore, we introduce additional depth hints supporting the user in understanding the spatial relationship of virtual waypoints in the physical world and investigate the effect of these visualization techniques on the spatial understanding.
A Real-Time Marker-Based Visual Sensor Based on a FPGA and a Soft Core Processor
Tayara, Hilal; Ham, Woonchul; Chong, Kil To
2016-01-01
This paper introduces a real-time marker-based visual sensor architecture for mobile robot localization and navigation. A hardware acceleration architecture for post video processing system was implemented on a field-programmable gate array (FPGA). The pose calculation algorithm was implemented in a System on Chip (SoC) with an Altera Nios II soft-core processor. For every frame, single pass image segmentation and Feature Accelerated Segment Test (FAST) corner detection were used for extracting the predefined markers with known geometries in FPGA. Coplanar PosIT algorithm was implemented on the Nios II soft-core processor supplied with floating point hardware for accelerating floating point operations. Trigonometric functions have been approximated using Taylor series and cubic approximation using Lagrange polynomials. Inverse square root method has been implemented for approximating square root computations. Real time results have been achieved and pixel streams have been processed on the fly without any need to buffer the input frame for further implementation. PMID:27983714
A Real-Time Marker-Based Visual Sensor Based on a FPGA and a Soft Core Processor.
Tayara, Hilal; Ham, Woonchul; Chong, Kil To
2016-12-15
This paper introduces a real-time marker-based visual sensor architecture for mobile robot localization and navigation. A hardware acceleration architecture for post video processing system was implemented on a field-programmable gate array (FPGA). The pose calculation algorithm was implemented in a System on Chip (SoC) with an Altera Nios II soft-core processor. For every frame, single pass image segmentation and Feature Accelerated Segment Test (FAST) corner detection were used for extracting the predefined markers with known geometries in FPGA. Coplanar PosIT algorithm was implemented on the Nios II soft-core processor supplied with floating point hardware for accelerating floating point operations. Trigonometric functions have been approximated using Taylor series and cubic approximation using Lagrange polynomials. Inverse square root method has been implemented for approximating square root computations. Real time results have been achieved and pixel streams have been processed on the fly without any need to buffer the input frame for further implementation.
PDF-modulated visual inputs and cryptochrome define diurnal behavior in Drosophila.
Cusumano, Paola; Klarsfeld, André; Chélot, Elisabeth; Picot, Marie; Richier, Benjamin; Rouyer, François
2009-11-01
Morning and evening circadian oscillators control the bimodal activity of Drosophila in light-dark cycles. The lateral neurons evening oscillator (LN-EO) is important for promoting diurnal activity at dusk. We found that the LN-EO autonomously synchronized to light-dark cycles through either the cryptochrome (CRY) that it expressed or the visual system. In conditions in which CRY was not activated, flies depleted for pigment-dispersing factor (PDF) or its receptor lost the evening activity and displayed reversed PER oscillations in the LN-EO. Rescue experiments indicated that normal PER cycling and the presence of evening activity relied on PDF secretion from the large ventral lateral neurons and PDF receptor function in the LN-EO. The LN-EO thus integrates light inputs and PDF signaling to control Drosophila diurnal behavior, revealing a new clock-independent function for PDF.
Measuring pilot workload in a motion base simulator. III - Synchronous secondary task
NASA Technical Reports Server (NTRS)
Kantowitz, Barry H.; Bortolussi, Michael R.; Hart, Sandra G.
1987-01-01
This experiment continues earlier research of Kantowitz et al. (1983) conducted in a GAT-1 motion-base trainer to evaluate choice-reaction secondary tasks as measures of pilot work load. The earlier work used an asynchronous secondary task presented every 22 sec regardless of flying performance. The present experiment uses a synchronous task presented only when a critical event occurred on the flying task. Both two- and four-choice visual secondary tasks were investigated. Analysis of primary flying-task results showed no decrement in error for altitude, indicating that the key assumption necessary for using a choice secondary task was satisfied. Reaction times showed significant differences between 'easy' and 'hard' flight scenarios as well as the ability to discriminate among flight tasks.
NASA Astrophysics Data System (ADS)
Du, Hai; Shi, Zhiwei; Cheng, Keming; Wei, Dechen; Li, Zheng; Zhou, Danjie; He, Haibo; Yao, Junkai; He, Chengjun
2016-06-01
Vortex control is a thriving research area, particularly in relation to flying wing or delta wing aircraft. This paper presents the topological structures of vortex flow on a flying wing aircraft controlled by a nanosecond plasma dielectric barrier discharge actuator. Experiments, including oil flow visualization and two-dimensional particle image velocimetry (PIV), were conducted in a wind tunnel with a Reynolds number of 0.5 × 106. Both oil and PIV results show that the vortex can be controlled. Oil topological structures on the aircraft surface coincide with spatial PIV flow structures. Both indicate vortex convergence and enhancement when the plasma discharge is switched on, leading to a reduced region of separated flow.
Local motion adaptation enhances the representation of spatial structure at EMD arrays
Lindemann, Jens P.; Egelhaaf, Martin
2017-01-01
Neuronal representation and extraction of spatial information are essential for behavioral control. For flying insects, a plausible way to gain spatial information is to exploit distance-dependent optic flow that is generated during translational self-motion. Optic flow is computed by arrays of local motion detectors retinotopically arranged in the second neuropile layer of the insect visual system. These motion detectors have adaptive response characteristics, i.e. their responses to motion with a constant or only slowly changing velocity decrease, while their sensitivity to rapid velocity changes is maintained or even increases. We analyzed by a modeling approach how motion adaptation affects signal representation at the output of arrays of motion detectors during simulated flight in artificial and natural 3D environments. We focused on translational flight, because spatial information is only contained in the optic flow induced by translational locomotion. Indeed, flies, bees and other insects segregate their flight into relatively long intersaccadic translational flight sections interspersed with brief and rapid saccadic turns, presumably to maximize periods of translation (80% of the flight). With a novel adaptive model of the insect visual motion pathway we could show that the motion detector responses to background structures of cluttered environments are largely attenuated as a consequence of motion adaptation, while responses to foreground objects stay constant or even increase. This conclusion even holds under the dynamic flight conditions of insects. PMID:29281631
Color Difference and Memory Recall in Free-Flying Honeybees: Forget the Hard Problem
Dyer, Adrian G.; Garcia, Jair E.
2014-01-01
Free-flying honeybees acquire color information differently depending upon whether a target color is learnt in isolation (absolute conditioning), or in relation to a perceptually similar color (differential conditioning). Absolute conditioning allows for rapid learning, but color discrimination is coarse. Differential conditioning requires more learning trials, but enables fine discriminations. Currently it is unknown whether differential conditioning to similar colors in honeybees forms a long-term memory, and the stability of memory in a biologically relevant scenario considering similar or saliently different color stimuli. Individual free-flying honeybees (N = 6) were trained to similar color stimuli separated by 0.06 hexagon units for 60 trials and mean accuracy was 81.7% ± 12.2% s.d. Bees retested on subsequent days showed a reduction in the number of correct choices with increasing time from the initial training, and for four of the bees this reduction was significant from chance expectation considering binomially distributed logistic regression models. In contrast, an independent group of 6 bees trained to saliently different colors (>0.14 hexagon units) did not experience any decay in memory retention with increasing time. This suggests that whilst the bees’ visual system can permit fine discriminations, flowers producing saliently different colors are more easily remembered by foraging bees over several days. PMID:26462830
Color Difference and Memory Recall in Free-Flying Honeybees: Forget the Hard Problem.
Dyer, Adrian G; Garcia, Jair E
2014-07-30
Free-flying honeybees acquire color information differently depending upon whether a target color is learnt in isolation (absolute conditioning), or in relation to a perceptually similar color (differential conditioning). Absolute conditioning allows for rapid learning, but color discrimination is coarse. Differential conditioning requires more learning trials, but enables fine discriminations. Currently it is unknown whether differential conditioning to similar colors in honeybees forms a long-term memory, and the stability of memory in a biologically relevant scenario considering similar or saliently different color stimuli. Individual free-flying honeybees (N = 6) were trained to similar color stimuli separated by 0.06 hexagon units for 60 trials and mean accuracy was 81.7% ± 12.2% s.d. Bees retested on subsequent days showed a reduction in the number of correct choices with increasing time from the initial training, and for four of the bees this reduction was significant from chance expectation considering binomially distributed logistic regression models. In contrast, an independent group of 6 bees trained to saliently different colors (>0.14 hexagon units) did not experience any decay in memory retention with increasing time. This suggests that whilst the bees' visual system can permit fine discriminations, flowers producing saliently different colors are more easily remembered by foraging bees over several days.
Neurokernel: An Open Source Platform for Emulating the Fruit Fly Brain
2016-01-01
We have developed an open software platform called Neurokernel for collaborative development of comprehensive models of the brain of the fruit fly Drosophila melanogaster and their execution and testing on multiple Graphics Processing Units (GPUs). Neurokernel provides a programming model that capitalizes upon the structural organization of the fly brain into a fixed number of functional modules to distinguish between these modules’ local information processing capabilities and the connectivity patterns that link them. By defining mandatory communication interfaces that specify how data is transmitted between models of each of these modules regardless of their internal design, Neurokernel explicitly enables multiple researchers to collaboratively model the fruit fly’s entire brain by integration of their independently developed models of its constituent processing units. We demonstrate the power of Neurokernel’s model integration by combining independently developed models of the retina and lamina neuropils in the fly’s visual system and by demonstrating their neuroinformation processing capability. We also illustrate Neurokernel’s ability to take advantage of direct GPU-to-GPU data transfers with benchmarks that demonstrate scaling of Neurokernel’s communication performance both over the number of interface ports exposed by an emulation’s constituent modules and the total number of modules comprised by an emulation. PMID:26751378
NASA Technical Reports Server (NTRS)
Hindson, W. S.; Hardy, G. H.; Innis, R. C.
1981-01-01
Flight tests were carried out to assess the feasibility of piloted steep curved, and decelerating approach profiles in powered lift STOL aircraft. Several STOL control concepts representative of a variety of aircraft were evaluated in conjunction with suitably designed flight directions. The tests were carried out in a real navigation environment, employed special electronic cockpit displays, and included the development of the performance achieved and the control utilization involved in flying 180 deg turning, descending, and decelerating approach profiles to landing. The results suggest that such moderately complex piloted instrument approaches may indeed be feasible from a pilot acceptance point of view, given an acceptable navigation environment. Systems with the capability of those used in this experiment can provide the potential of achieving instrument operations on curved, descending, and decelerating landing approaches to weather minima corresponding to CTOL Category 2 criteria, while also providing a means of realizing more efficient operations during visual flight conditions.
NASA Astrophysics Data System (ADS)
Nath, D. C. D.; Bandyopadhyay, S.; Campbell, J.; Yu, A.; Blackburn, D.; White, C.
2010-12-01
Composite films of poly(vinyl alcohol) (PVA) reinforced with 5, 10, 15, 20 and 25 wt.% surface-coated fly ash by surfactant, sodium lauryl sulphate (SLS-FA) along with 1 wt.% cross-linking agent, glutaraldehyde (GLA) were prepared by aqueous casting method. The tensile strengths of the composite films were increased proportionally with the addition of SLS-FA. The maximum 75% higher strength of the composite with 20 wt.% was achieved compared to that of neat PVA. The modulus of the composites was also increased proportionally with SLS-FA and the maximum 218% reached in composite with 20 wt.%, but the strain at break was decreased with addition of SLS-FA. Changes in FTIR spectra reflect the chemical and/or physical bonding in the ternary PVA, SLS-FA and GLA component systems. In the study of surface morphology, the connectivity was visualized in SEM images along with interstitial voids. The films with SLS-FA show 53% smoother surface calculated with AFM compared to unmodified FA composite films.
Ko Displacement Theory for Structural Shape Predictions
NASA Technical Reports Server (NTRS)
Ko, William L.
2010-01-01
The development of the Ko displacement theory for predictions of structure deformed shapes was motivated in 2003 by the Helios flying wing, which had a 247-ft (75-m) wing span with wingtip deflections reaching 40 ft (12 m). The Helios flying wing failed in midair in June 2003, creating the need to develop new technology to predict in-flight deformed shapes of unmanned aircraft wings for visual display before the ground-based pilots. Any types of strain sensors installed on a structure can only sense the surface strains, but are incapable to sense the overall deformed shapes of structures. After the invention of the Ko displacement theory, predictions of structure deformed shapes could be achieved by feeding the measured surface strains into the Ko displacement transfer functions for the calculations of out-of-plane deflections and cross sectional rotations at multiple locations for mapping out overall deformed shapes of the structures. The new Ko displacement theory combined with a strain-sensing system thus created a revolutionary new structure- shape-sensing technology.
Adhesion design maps for bio-inspired attachment systems.
Spolenak, Ralph; Gorb, Stanislav; Arzt, Eduard
2005-01-01
Fibrous surface structures can improve the adhesion of objects to other surfaces. Animals, such as flies and geckos, take advantage of this principle by developing "hairy" contact structures which ensure controlled and repeatable adhesion and detachment. Mathematical models for fiber adhesion predict pronounced dependencies of contact performance on the geometry and the elastic properties of the fibers. In this paper the limits of such contacts imposed by fiber strength, fiber condensation, compliance, and ideal contact strength are modeled for spherical contact tips. Based on this, we introduce the concept of "adhesion design maps" which visualize the predicted mechanical behavior. The maps are useful for understanding biological systems and for guiding experimentation to achieve optimum artificial contacts.
Parallel Computations in Insect and Mammalian Visual Motion Processing
Clark, Damon A.; Demb, Jonathan B.
2016-01-01
Sensory systems use receptors to extract information from the environment and neural circuits to perform subsequent computations. These computations may be described as algorithms composed of sequential mathematical operations. Comparing these operations across taxa reveals how different neural circuits have evolved to solve the same problem, even when using different mechanisms to implement the underlying math. In this review, we compare how insect and mammalian neural circuits have solved the problem of motion estimation, focusing on the fruit fly Drosophila and the mouse retina. Although the two systems implement computations with grossly different anatomy and molecular mechanisms, the underlying circuits transform light into motion signals with strikingly similar processing steps. These similarities run from photoreceptor gain control and spatiotemporal tuning to ON and OFF pathway structures, motion detection, and computed motion signals. The parallels between the two systems suggest that a limited set of algorithms for estimating motion satisfies both the needs of sighted creatures and the constraints imposed on them by metabolism, anatomy, and the structure and regularities of the visual world. PMID:27780048
Parallel Computations in Insect and Mammalian Visual Motion Processing.
Clark, Damon A; Demb, Jonathan B
2016-10-24
Sensory systems use receptors to extract information from the environment and neural circuits to perform subsequent computations. These computations may be described as algorithms composed of sequential mathematical operations. Comparing these operations across taxa reveals how different neural circuits have evolved to solve the same problem, even when using different mechanisms to implement the underlying math. In this review, we compare how insect and mammalian neural circuits have solved the problem of motion estimation, focusing on the fruit fly Drosophila and the mouse retina. Although the two systems implement computations with grossly different anatomy and molecular mechanisms, the underlying circuits transform light into motion signals with strikingly similar processing steps. These similarities run from photoreceptor gain control and spatiotemporal tuning to ON and OFF pathway structures, motion detection, and computed motion signals. The parallels between the two systems suggest that a limited set of algorithms for estimating motion satisfies both the needs of sighted creatures and the constraints imposed on them by metabolism, anatomy, and the structure and regularities of the visual world. Copyright © 2016 Elsevier Ltd. All rights reserved.
A Hybrid Synthetic Vision System for the Tele-operation of Unmanned Vehicles
NASA Technical Reports Server (NTRS)
Delgado, Frank; Abernathy, Mike
2004-01-01
A system called SmartCam3D (SC3D) has been developed to provide enhanced situational awareness for operators of a remotely piloted vehicle. SC3D is a Hybrid Synthetic Vision System (HSVS) that combines live sensor data with information from a Synthetic Vision System (SVS). By combining the dual information sources, the operators are afforded the advantages of each approach. The live sensor system provides real-time information for the region of interest. The SVS provides information rich visuals that will function under all weather and visibility conditions. Additionally, the combination of technologies allows the system to circumvent some of the limitations from each approach. Video sensor systems are not very useful when visibility conditions are hampered by rain, snow, sand, fog, and smoke, while a SVS can suffer from data freshness problems. Typically, an aircraft or satellite flying overhead collects the data used to create the SVS visuals. The SVS data could have been collected weeks, months, or even years ago. To that extent, the information from an SVS visual could be outdated and possibly inaccurate. SC3D was used in the remote cockpit during flight tests of the X-38 132 and 131R vehicles at the NASA Dryden Flight Research Center. SC3D was also used during the operation of military Unmanned Aerial Vehicles. This presentation will provide an overview of the system, the evolution of the system, the results of flight tests, and future plans. Furthermore, the safety benefits of the SC3D over traditional and pure synthetic vision systems will be discussed.
Bleul, Christiane; Baumann-Klausener, Franziska; Labhart, Thomas; Dickinson, Michael H.
2016-01-01
Many insects exploit skylight polarization as a compass cue for orientation and navigation. In the fruit fly, Drosophila melanogaster, photoreceptors R7 and R8 in the dorsal rim area (DRA) of the compound eye are specialized to detect the electric vector (e-vector) of linearly polarized light. These photoreceptors are arranged in stacked pairs with identical fields of view and spectral sensitivities, but mutually orthogonal microvillar orientations. As in larger flies, we found that the microvillar orientation of the distal photoreceptor R7 changes in a fan-like fashion along the DRA. This anatomical arrangement suggests that the DRA constitutes a detector for skylight polarization, in which different e-vectors maximally excite different positions in the array. To test our hypothesis, we measured responses to polarized light of varying e-vector angles in the terminals of R7/8 cells using genetically encoded calcium indicators. Our data confirm a progression of preferred e-vector angles from anterior to posterior in the DRA, and a strict orthogonality between the e-vector preferences of paired R7/8 cells. We observed decreased activity in photoreceptors in response to flashes of light polarized orthogonally to their preferred e-vector angle, suggesting reciprocal inhibition between photoreceptors in the same medullar column, which may serve to increase polarization contrast. Together, our results indicate that the polarization-vision system relies on a spatial map of preferred e-vector angles at the earliest stage of sensory processing. SIGNIFICANCE STATEMENT The fly's visual system is an influential model system for studying neural computation, and much is known about its anatomy, physiology, and development. The circuits underlying motion processing have received the most attention, but researchers are increasingly investigating other functions, such as color perception and object recognition. In this work, we investigate the early neural processing of a somewhat exotic sense, called polarization vision. Because skylight is polarized in an orientation that is rigidly determined by the position of the sun, this cue provides compass information. Behavioral experiments have shown that many species use the polarization pattern in the sky to direct locomotion. Here we describe the input stage of the fly's polarization-vision system. PMID:27170135
Visual-conformal display format for helicopter guidance
NASA Astrophysics Data System (ADS)
Doehler, H.-U.; Schmerwitz, Sven; Lueken, Thomas
2014-06-01
Helicopter guidance in situations where natural vision is reduced is still a challenging task. Beside new available sensors, which are able to "see" through darkness, fog and dust, display technology remains one of the key issues of pilot assistance systems. As long as we have pilots within aircraft cockpits, we have to keep them informed about the outside situation. "Situational awareness" of humans is mainly powered by their visual channel. Therefore, display systems which are able to cross-fade seamless from natural vision to artificial computer vision and vice versa, are of greatest interest within this context. Helmet-mounted displays (HMD) have this property when they apply a head-tracker for measuring the pilot's head orientation relative to the aircraft reference frame. Together with the aircraft's position and orientation relative to the world's reference frame, the on-board graphics computer can generate images which are perfectly aligned with the outside world. We call image elements which match the outside world, "visual-conformal". Published display formats for helicopter guidance in degraded visual environment apply mostly 2D-symbologies which stay far behind from what is possible. We propose a perspective 3D-symbology for a head-tracked HMD which shows as much as possible visual-conformal elements. We implemented and tested our proposal within our fixed based cockpit simulator as well as in our flying helicopter simulator (FHS). Recently conducted simulation trials with experienced helicopter pilots give some first evaluation results of our proposal.
Altitude control in honeybees: joint vision-based learning and guidance.
Portelli, Geoffrey; Serres, Julien R; Ruffier, Franck
2017-08-23
Studies on insects' visual guidance systems have shed little light on how learning contributes to insects' altitude control system. In this study, honeybees were trained to fly along a double-roofed tunnel after entering it near either the ceiling or the floor of the tunnel. The honeybees trained to hug the ceiling therefore encountered a sudden change in the tunnel configuration midways: i.e. a "dorsal ditch". Thus, the trained honeybees met a sudden increase in the distance to the ceiling, corresponding to a sudden strong change in the visual cues available in their dorsal field of view. Honeybees reacted by rising quickly and hugging the new, higher ceiling, keeping a similar forward speed, distance to the ceiling and dorsal optic flow to those observed during the training step; whereas bees trained to follow the floor kept on following the floor regardless of the change in the ceiling height. When trained honeybees entered the tunnel via the other entry (the lower or upper entry) to that used during the training step, they quickly changed their altitude and hugged the surface they had previously learned to follow. These findings clearly show that trained honeybees control their altitude based on visual cues memorized during training. The memorized visual cues generated by the surfaces followed form a complex optic flow pattern: trained honeybees may attempt to match the visual cues they perceive with this memorized optic flow pattern by controlling their altitude.
Newton, Jenny; Barrett, Steven F; Wilcox, Michael J; Popp, Stephanie
2002-01-01
Machine vision for navigational purposes is a rapidly growing field. Many abilities such as object recognition and target tracking rely on vision. Autonomous vehicles must be able to navigate in dynamic enviroments and simultaneously locate a target position. Traditional machine vision often fails to react in real time because of large computational requirements whereas the fly achieves complex orientation and navigation with a relatively small and simple brain. Understanding how the fly extracts visual information and how neurons encode and process information could lead us to a new approach for machine vision applications. Photoreceptors in the Musca domestica eye that share the same spatial information converge into a structure called the cartridge. The cartridge consists of the photoreceptor axon terminals and monopolar cells L1, L2, and L4. It is thought that L1 and L2 cells encode edge related information relative to a single cartridge. These cells are thought to be equivalent to vertebrate bipolar cells, producing contrast enhancement and reduction of information sent to L4. Monopolar cell L4 is thought to perform image segmentation on the information input from L1 and L2 and also enhance edge detection. A mesh of interconnected L4's would correlate the output from L1 and L2 cells of adjacent cartridges and provide a parallel network for segmenting an object's edges. The focus of this research is to excite photoreceptors of the common housefly, Musca domestica, with different visual patterns. The electrical response of monopolar cells L1, L2, and L4 will be recorded using intracellular recording techniques. Signal analysis will determine the neurocircuitry to detect and segment images.
Nonlinear Flying Qualities Criteria for Large-Amplitude Maneuvers
1984-12-01
theory which are pertinent to the formation of a nonlinear flying qualities methodology. This report surveys nonlinear system theory and describes...the development of an applied flying qualities methodology based on a canonical system theory and using research in relative controllability...The Nonlinear Flying Qualities (NFQ) for Large-Amplitude Maneuvers Program examined promising techniques from nonlinear analysis and nonlinear system
A Vision-Based Counting and Recognition System for Flying Insects in Intelligent Agriculture.
Zhong, Yuanhong; Gao, Junyuan; Lei, Qilun; Zhou, Yao
2018-05-09
Rapid and accurate counting and recognition of flying insects are of great importance, especially for pest control. Traditional manual identification and counting of flying insects is labor intensive and inefficient. In this study, a vision-based counting and classification system for flying insects is designed and implemented. The system is constructed as follows: firstly, a yellow sticky trap is installed in the surveillance area to trap flying insects and a camera is set up to collect real-time images. Then the detection and coarse counting method based on You Only Look Once (YOLO) object detection, the classification method and fine counting based on Support Vector Machines (SVM) using global features are designed. Finally, the insect counting and recognition system is implemented on Raspberry PI. Six species of flying insects including bee, fly, mosquito, moth, chafer and fruit fly are selected to assess the effectiveness of the system. Compared with the conventional methods, the test results show promising performance. The average counting accuracy is 92.50% and average classifying accuracy is 90.18% on Raspberry PI. The proposed system is easy-to-use and provides efficient and accurate recognition data, therefore, it can be used for intelligent agriculture applications.
A Vision-Based Counting and Recognition System for Flying Insects in Intelligent Agriculture
Zhong, Yuanhong; Gao, Junyuan; Lei, Qilun; Zhou, Yao
2018-01-01
Rapid and accurate counting and recognition of flying insects are of great importance, especially for pest control. Traditional manual identification and counting of flying insects is labor intensive and inefficient. In this study, a vision-based counting and classification system for flying insects is designed and implemented. The system is constructed as follows: firstly, a yellow sticky trap is installed in the surveillance area to trap flying insects and a camera is set up to collect real-time images. Then the detection and coarse counting method based on You Only Look Once (YOLO) object detection, the classification method and fine counting based on Support Vector Machines (SVM) using global features are designed. Finally, the insect counting and recognition system is implemented on Raspberry PI. Six species of flying insects including bee, fly, mosquito, moth, chafer and fruit fly are selected to assess the effectiveness of the system. Compared with the conventional methods, the test results show promising performance. The average counting accuracy is 92.50% and average classifying accuracy is 90.18% on Raspberry PI. The proposed system is easy-to-use and provides efficient and accurate recognition data, therefore, it can be used for intelligent agriculture applications. PMID:29747429
The Killer Fly Hunger Games: Target Size and Speed Predict Decision to Pursuit
Wardill, Trevor J.; Knowles, Katie; Barlow, Laura; Tapia, Gervasio; Nordström, Karin; Olberg, Robert M.; Gonzalez-Bellido, Paloma T.
2015-01-01
Predatory animals have evolved to optimally detect their prey using exquisite sensory systems such as vision, olfaction and hearing. It may not be so surprising that vertebrates, with large central nervous systems, excel at predatory behaviors. More striking is the fact that many tiny insects, with their miniscule brains and scaled down nerve cords, are also ferocious, highly successful predators. For predation, it is important to determine whether a prey is suitable before initiating pursuit. This is paramount since pursuing a prey that is too large to capture, subdue or dispatch will generate a substantial metabolic cost (in the form of muscle output) without any chance of metabolic gain (in the form of food). In addition, during all pursuits, the predator breaks its potential camouflage and thus runs the risk of becoming prey itself. Many insects use their eyes to initially detect and subsequently pursue prey. Dragonflies, which are extremely efficient predators, therefore have huge eyes with relatively high spatial resolution that allow efficient prey size estimation before initiating pursuit. However, much smaller insects, such as killer flies, also visualize and successfully pursue prey. This is an impressive behavior since the small size of the killer fly naturally limits the neural capacity and also the spatial resolution provided by the compound eye. Despite this, we here show that killer flies efficiently pursue natural (Drosophila melanogaster) and artificial (beads) prey. The natural pursuits are initiated at a distance of 7.9 ± 2.9 cm, which we show is too far away to allow for distance estimation using binocular disparities. Moreover, we show that rather than estimating absolute prey size prior to launching the attack, as dragonflies do, killer flies attack with high probability when the ratio of the prey's subtended retinal velocity and retinal size is 0.37. We also show that killer flies will respond to a stimulus of an angular size that is smaller than that of the photoreceptor acceptance angle, and that the predatory response is strongly modulated by the metabolic state. Our data thus provide an exciting example of a loosely designed matched filter to Drosophila, but one which will still generate successful pursuits of other suitable prey. PMID:26398293
Catching fly balls in virtual reality: a critical test of the outfielder problem.
Fink, Philip W; Foo, Patrick S; Warren, William H
2009-12-14
How does a baseball outfielder know where to run to catch a fly ball? The "outfielder problem" remains unresolved, and its solution would provide a window into the visual control of action. It may seem obvious that human action is based on an internal model of the physical world, such that the fielder predicts the landing point based on a mental model of the ball's trajectory (TP). However, two alternative theories, Optical Acceleration Cancellation (OAC) and Linear Optical Trajectory (LOT), propose that fielders are led to the right place at the right time by coupling their movements to visual information in a continuous "online" manner. All three theories predict successful catches and similar running paths. We provide a critical test by using virtual reality to perturb the vertical motion of the ball in mid-flight. The results confirm the predictions of OAC but are at odds with LOT and TP.
Proceedings from the 2nd International Symposium on Formation Flying Missions and Technologies
NASA Technical Reports Server (NTRS)
2004-01-01
Topics discussed include: The Stellar Imager (SI) "Vision Mission"; First Formation Flying Demonstration Mission Including on Flight Nulling; Formation Flying X-ray Telescope in L2 Orbit; SPECS: The Kilometer-baseline Far-IR Interferometer in NASA's Space Science Roadmap Presentation; A Tight Formation for Along-track SAR Interferometry; Realization of the Solar Power Satellite using the Formation Flying Solar Reflector; SIMBOL-X : Formation Flying for High-Energy Astrophysics; High Precision Optical Metrology for DARWIN; Close Formation Flight of Micro-Satellites for SAR Interferometry; Station-Keeping Requirements for Astronomical Imaging with Constellations of Free-Flying Collectors; Closed-Loop Control of Formation Flying Satellites; Formation Control for the MAXIM Mission; Precision Formation Keeping at L2 Using the Autonomous Formation Flying Sensor; Robust Control of Multiple Spacecraft Formation Flying; Virtual Rigid Body (VRB) Satellite Formation Control: Stable Mode-Switching and Cross-Coupling; Electromagnetic Formation Flight (EMFF) System Design, Mission Capabilities, and Testbed Development; Navigation Algorithms for Formation Flying Missions; Use of Formation Flying Small Satellites Incorporating OISL's in a Tandem Cluster Mission; Semimajor Axis Estimation Strategies; Relative Attitude Determination of Earth Orbiting Formations Using GPS Receivers; Analysis of Formation Flying in Eccentric Orbits Using Linearized Equations of Relative Motion; Conservative Analytical Collision Probabilities for Orbital Formation Flying; Equations of Motion and Stability of Two Spacecraft in Formation at the Earth/Moon Triangular Libration Points; Formations Near the Libration Points: Design Strategies Using Natural and Non-Natural Ares; An Overview of the Formation and Attitude Control System for the Terrestrial Planet Finder Formation Flying Interferometer; GVE-Based Dynamics and Control for Formation Flying Spacecraft; GNC System Design for a New Concept of X-Ray Distributed Telescope; GNC System for the Deployment and Fine Control of the DARWIN Free-Flying Interferometer; Formation Algorithm and Simulation Testbed; and PLATFORM: A Formation Flying, RvD and Robotic Validation Test-bench.
Evaluation of a GPS used in conjunction with aerial telemetry
Olexa, E.M.; Gogan, P.J.P.; Podruzny, K.M.; Eiler, John; Alcorn, Doris J.; Neuman, Michael R.
2001-01-01
We investigated the use of a non-correctable Global Positioning System (NGPS) in association with aerial telemetry to determine animal locations. Average error was determined for 3 components of the location process: use of a NGPS receiver on the ground, use of a NGPS receiver in a aircraft while flying over a visual marker, and use of the same receiver while flying over a location determined by standard aerial telemetry. Average errors were 45.3, 88.1 and 137.4 m, respectively. A directional bias of <35 m was present for the telemetry component only. Tests indicated that use of NGPS to determine aircraft, and thereby animal, location is an efficient alternative to interpolation from topographic maps. This method was more accurate than previously reported Long-Range Navigation system, version C (LORAN-C) and Argos satellite telemetry. It has utility in areas where animal-borne GPS receivers are not practical due to a combination of topography, canopy coverage, weight or cost of animal-borne GPS units. Use of NGPS technology in conjunction with aerial telemetry will provide the location accuracy required for identification of gross movement patterns and coarse-grained habitat use.
Sensor fusion in identified visual interneurons.
Parsons, Matthew M; Krapp, Holger G; Laughlin, Simon B
2010-04-13
Animal locomotion often depends upon stabilization reflexes that use sensory feedback to maintain trajectories and orientation. Such stabilizing reflexes are critically important for the blowfly, whose aerodynamic instability permits outstanding maneuverability but increases the demands placed on flight control. Flies use several sensory systems to drive reflex responses, and recent studies have provided access to the circuitry responsible for combining and employing these sensory inputs. We report that lobula plate VS neurons combine inputs from two optical sensors, the ocelli and the compound eyes. Both systems deliver essential information on in-flight rotations, but our neuronal recordings reveal that the ocelli encode this information in three axes, whereas the compound eyes encode in nine. The difference in dimensionality is reconciled by tuning each VS neuron to the ocellar axis closest to its compound eye axis. We suggest that this simple projection combines the speed of the ocelli with the accuracy of the compound eyes without compromising either. Our findings also support the suggestion that the coordinates of sensory information processing are aligned with axes controlling the natural modes of the fly's flight to improve the efficiency with which sensory signals are transformed into appropriate motor commands.
Filling gaps in visual motion for target capture
Bosco, Gianfranco; Delle Monache, Sergio; Gravano, Silvio; Indovina, Iole; La Scaleia, Barbara; Maffei, Vincenzo; Zago, Myrka; Lacquaniti, Francesco
2015-01-01
A remarkable challenge our brain must face constantly when interacting with the environment is represented by ambiguous and, at times, even missing sensory information. This is particularly compelling for visual information, being the main sensory system we rely upon to gather cues about the external world. It is not uncommon, for example, that objects catching our attention may disappear temporarily from view, occluded by visual obstacles in the foreground. Nevertheless, we are often able to keep our gaze on them throughout the occlusion or even catch them on the fly in the face of the transient lack of visual motion information. This implies that the brain can fill the gaps of missing sensory information by extrapolating the object motion through the occlusion. In recent years, much experimental evidence has been accumulated that both perceptual and motor processes exploit visual motion extrapolation mechanisms. Moreover, neurophysiological and neuroimaging studies have identified brain regions potentially involved in the predictive representation of the occluded target motion. Within this framework, ocular pursuit and manual interceptive behavior have proven to be useful experimental models for investigating visual extrapolation mechanisms. Studies in these fields have pointed out that visual motion extrapolation processes depend on manifold information related to short-term memory representations of the target motion before the occlusion, as well as to longer term representations derived from previous experience with the environment. We will review recent oculomotor and manual interception literature to provide up-to-date views on the neurophysiological underpinnings of visual motion extrapolation. PMID:25755637
Filling gaps in visual motion for target capture.
Bosco, Gianfranco; Monache, Sergio Delle; Gravano, Silvio; Indovina, Iole; La Scaleia, Barbara; Maffei, Vincenzo; Zago, Myrka; Lacquaniti, Francesco
2015-01-01
A remarkable challenge our brain must face constantly when interacting with the environment is represented by ambiguous and, at times, even missing sensory information. This is particularly compelling for visual information, being the main sensory system we rely upon to gather cues about the external world. It is not uncommon, for example, that objects catching our attention may disappear temporarily from view, occluded by visual obstacles in the foreground. Nevertheless, we are often able to keep our gaze on them throughout the occlusion or even catch them on the fly in the face of the transient lack of visual motion information. This implies that the brain can fill the gaps of missing sensory information by extrapolating the object motion through the occlusion. In recent years, much experimental evidence has been accumulated that both perceptual and motor processes exploit visual motion extrapolation mechanisms. Moreover, neurophysiological and neuroimaging studies have identified brain regions potentially involved in the predictive representation of the occluded target motion. Within this framework, ocular pursuit and manual interceptive behavior have proven to be useful experimental models for investigating visual extrapolation mechanisms. Studies in these fields have pointed out that visual motion extrapolation processes depend on manifold information related to short-term memory representations of the target motion before the occlusion, as well as to longer term representations derived from previous experience with the environment. We will review recent oculomotor and manual interception literature to provide up-to-date views on the neurophysiological underpinnings of visual motion extrapolation.
Superior visual performance in nocturnal insects: neural principles and bio-inspired technologies
NASA Astrophysics Data System (ADS)
Warrant, Eric J.
2016-04-01
At night, our visual capacities are severely reduced, with a complete loss in our ability to see colour and a dramatic loss in our ability to see fine spatial and temporal details. This is not the case for many nocturnal animals, notably insects. Our recent work, particularly on fast-flying moths and bees and on ball-rolling dung beetles, has shown that nocturnal animals are able to distinguish colours, to detect faint movements, to learn visual landmarks, to orient to the faint pattern of polarised light produced by the moon and to navigate using the stars. These impressive visual abilities are the result of exquisitely adapted eyes and visual systems, the product of millions of years of evolution. Nocturnal animals typically have highly sensitive eye designs and visual neural circuitry that is optimised for extracting reliable information from dim and noisy visual images. Even though we are only at the threshold of understanding the neural mechanisms responsible for reliable nocturnal vision, growing evidence suggests that the neural summation of photons in space and time is critically important: even though vision in dim light becomes necessarily coarser and slower, it also becomes significantly more reliable. We explored the benefits of spatiotemporal summation by creating a computer algorithm that mimicked nocturnal visual processing strategies. This algorithm dramatically increased the reliability of video collected in dim light, including the preservation of colour, strengthening evidence that summation strategies are essential for nocturnal vision.
Shape and texture fused recognition of flying targets
NASA Astrophysics Data System (ADS)
Kovács, Levente; Utasi, Ákos; Kovács, Andrea; Szirányi, Tamás
2011-06-01
This paper presents visual detection and recognition of flying targets (e.g. planes, missiles) based on automatically extracted shape and object texture information, for application areas like alerting, recognition and tracking. Targets are extracted based on robust background modeling and a novel contour extraction approach, and object recognition is done by comparisons to shape and texture based query results on a previously gathered real life object dataset. Application areas involve passive defense scenarios, including automatic object detection and tracking with cheap commodity hardware components (CPU, camera and GPS).
Color polymorphic lures target different visual channels in prey.
White, Thomas E; Kemp, Darrell J
2016-06-01
Selection for signal efficacy in variable environments may favor color polymorphism, but little is known about this possibility outside of sexual systems. Here we used the color polymorphic orb-web spider Gasteracantha fornicata, whose yellow- or white-banded dorsal signal attracts dipteran prey, to test the hypothesis that morphs may be tuned to optimize either chromatic or achromatic conspicuousness in their visually noisy forest environments. We used data from extensive observations of naturally existing spiders and precise assessments of visual environments to model signal conspicuousness according to dipteran vision. Modeling supported a distinct bias in the chromatic (yellow morph) or achromatic (white morph) contrast presented by spiders at the times when they caught prey, as opposed to all other times at which they may be viewed. Hence, yellow spiders were most successful when their signal produced maximum color contrast against viewing backgrounds, whereas white spiders were most successful when they presented relatively greatest luminance contrast. Further modeling across a hypothetical range of lure variation confirmed that yellow versus white signals should, respectively, enhance chromatic versus achromatic conspicuousness to flies, in G. fornicata's visual environments. These findings suggest that color polymorphism may be adaptively maintained by selection for conspicuousness within different visual channels in receivers. © 2016 The Author(s). Evolution © 2016 The Society for the Study of Evolution.
The differential optomotor response of the four-eyed fish Anableps anableps.
Albensi, B C; Powell, J H
1998-01-01
The perception of motion is important for the survival and reproduction of many animals, including fish. In the laboratory, support for this idea comes from the observation that many fish show a tendency to follow a series of stripes revolving around a circular aquarium. This response, known as the optomotor response (OMR), is recognized as an innate behavior in many species. The 'four-eyed' fishes of the genus Anableps are an unusual fish from Central and South America and actually have only two eyes. Each eye is divided into upper and lower halves internally and externally. This peculiar dual visual system allows Anableps to feed on creatures that swim or land near or on the water surface or to flee from flying predators attacking from above. It was hypothesized that Anableps should also possess the OMR. We used the OMR as a test to investigate potential differential visual processing in Anableps on normal and 'blinded' fish (the eyes are actually covered--not physically blinded). It was found that the OMR does exist in Anableps and that the strength of this response is dependent on the visual field being tested--a stronger OMR was seen as a result of visual stimulation from the aerial environment.
Active eye-tracking improves LASIK results.
Lee, Yuan-Chieh
2007-06-01
To study the advantage of active eye-tracking for photorefractive surgery. In a prospective, double-masked study, LASIK for myopia and myopic astigmatism was performed in 50 patients using the ALLEGRETTO WAVE version 1007. All patients received LASIK with full comprehension of the importance of fixation during the procedure. All surgical procedures were performed by a single surgeon. The eye-tracker was turned off in one group (n = 25) and kept on in another group (n = 25). Preoperatively and 3 months postoperatively, patients underwent a standard ophthalmic examination, which included comeal topography. In the patients treated with the eye-tracker off, all had uncorrected visual acuity (UCVA) of > or = 20/40 and 64% had > or = 20/20. Compared with the patients treated with the eye-tracker on, they had higher residual cylindrical astigmatism (P < .05). Those treated with the eye-tracker on achieved better UCVA and best spectacle-corrected visual acuity (P < .05). Spherical error and potential visual acuity (TMS-II) were not significantly different between the groups. The flying-spot system can achieve a fair result without active eye-tracking, but active eye-tracking helps improve the visual outcome and reduces postoperative cylindrical astigmatism.
Mankin, R W; Anderson, J B; Mizrach, A; Epsky, N D; Shuman, D; Heath, R R; Mazor, M; Hetzroni, A; Grinshpun, J; Taylor, P W; Garrett, S L
2004-08-01
Female Mediterranean fruit flies, Ceratitis capitata (Wiedemann), from the sterile-male rearing facility in El Pino, Guatemala, were exposed to broadcasts of wing-fanning vibrations recorded from males engaged in calling behavior to investigate the feasibility of developing a female-selective acoustic trap. The recorded signals had frequent amplitude fluctuations and peak frequencies approximately 350 Hz, typical of signals observed in previous studies of Mediterranean fruit fly acoustic behavior. Females did not exhibit long-distance phonotaxis, but remained near a speaker significantly longer when the sounds were broadcast at 103-107 dB than when the speaker was silent. In addition, significantly higher percentages of females were captured by yellow adhesive traps next to a broadcasting speaker than by traps next to a silent mimic. Additional bioassays were conducted with synthetic, 350-Hz tones produced by a thermoacoustic tube as well as with silent mimics of the different sound sources to examine the relative responsiveness of female Mediterranean fruit flies to traps with different acoustic and visual features. The visual attributes of the different sound source assemblies significantly affected capture rates. The range over which the broadcast significantly increased the percentage of female captures was <0.5 m, which may limit the utility of these acoustic cues in large-scale trapping programs. However, the findings of this study do justify further testing of whether optimized short-range acoustic signals could be used to augment longer range pheromonal and visual cues to improve the efficacy of female-selective traps.
Bridging the Gap between NASA Hydrological Data and the Geospatial Community
NASA Technical Reports Server (NTRS)
Rui, Hualan; Teng, Bill; Vollmer, Bruce; Mocko, David M.; Beaudoing, Hiroko K.; Nigro, Joseph; Gary, Mark; Maidment, David; Hooper, Richard
2011-01-01
There is a vast and ever increasing amount of data on the Earth interconnected energy and hydrological systems, available from NASA remote sensing and modeling systems, and yet, one challenge persists: increasing the usefulness of these data for, and thus their use by, the geospatial communities. The Hydrology Data and Information Services Center (HDISC), part of the Goddard Earth Sciences DISC, has continually worked to better understand the hydrological data needs of the geospatial end users, to thus better able to bridge the gap between NASA data and the geospatial communities. This paper will cover some of the hydrological data sets available from HDISC, and the various tools and services developed for data searching, data subletting ; format conversion. online visualization and analysis; interoperable access; etc.; to facilitate the integration of NASA hydrological data by end users. The NASA Goddard data analysis and visualization system, Giovanni, is described. Two case examples of user-customized data services are given, involving the EPA BASINS (Better Assessment Science Integrating point & Non-point Sources) project and the CUAHSI Hydrologic Information System, with the common requirement of on-the-fly retrieval of long duration time series for a geographical point
Assessing Dual Sensor Enhanced Flight Vision Systems to Enable Equivalent Visual Operations
NASA Technical Reports Server (NTRS)
Kramer, Lynda J.; Etherington, Timothy J.; Severance, Kurt; Bailey, Randall E.; Williams, Steven P.; Harrison, Stephanie J.
2016-01-01
Flight deck-based vision system technologies, such as Synthetic Vision (SV) and Enhanced Flight Vision Systems (EFVS), may serve as a revolutionary crew/vehicle interface enabling technologies to meet the challenges of the Next Generation Air Transportation System Equivalent Visual Operations (EVO) concept - that is, the ability to achieve the safety of current-day Visual Flight Rules (VFR) operations and maintain the operational tempos of VFR irrespective of the weather and visibility conditions. One significant challenge lies in the definition of required equipage on the aircraft and on the airport to enable the EVO concept objective. A motion-base simulator experiment was conducted to evaluate the operational feasibility, pilot workload and pilot acceptability of conducting straight-in instrument approaches with published vertical guidance to landing, touchdown, and rollout to a safe taxi speed in visibility as low as 300 ft runway visual range by use of onboard vision system technologies on a Head-Up Display (HUD) without need or reliance on natural vision. Twelve crews evaluated two methods of combining dual sensor (millimeter wave radar and forward looking infrared) EFVS imagery on pilot-flying and pilot-monitoring HUDs as they made approaches to runways with and without touchdown zone and centerline lights. In addition, the impact of adding SV to the dual sensor EFVS imagery on crew flight performance, workload, and situation awareness during extremely low visibility approach and landing operations was assessed. Results indicate that all EFVS concepts flown resulted in excellent approach path tracking and touchdown performance without any workload penalty. Adding SV imagery to EFVS concepts provided situation awareness improvements but no discernible improvements in flight path maintenance.
Multiple Drosophila Tracking System with Heading Direction
Sirigrivatanawong, Pudith; Arai, Shogo; Thoma, Vladimiros; Hashimoto, Koichi
2017-01-01
Machine vision systems have been widely used for image analysis, especially that which is beyond human ability. In biology, studies of behavior help scientists to understand the relationship between sensory stimuli and animal responses. This typically requires the analysis and quantification of animal locomotion. In our work, we focus on the analysis of the locomotion of the fruit fly Drosophila melanogaster, a widely used model organism in biological research. Our system consists of two components: fly detection and tracking. Our system provides the ability to extract a group of flies as the objects of concern and furthermore determines the heading direction of each fly. As each fly moves, the system states are refined with a Kalman filter to obtain the optimal estimation. For the tracking step, combining information such as position and heading direction with assignment algorithms gives a successful tracking result. The use of heading direction increases the system efficiency when dealing with identity loss and flies swapping situations. The system can also operate with a variety of videos with different light intensities. PMID:28067800
Global versus local adaptation in fly motion-sensitive neurons
Neri, Peter; Laughlin, Simon B
2005-01-01
Flies, like humans, experience a well-known consequence of adaptation to visual motion, the waterfall illusion. Direction-selective neurons in the fly lobula plate permit a detailed analysis of the mechanisms responsible for motion adaptation and their function. Most of these neurons are spatially non-opponent, they sum responses to motion in the preferred direction across their entire receptive field, and adaptation depresses responses by subtraction and by reducing contrast gain. When we adapted a small area of the receptive field to motion in its anti-preferred direction, we discovered that directional gain at unadapted regions was enhanced. This novel phenomenon shows that neuronal responses to the direction of stimulation in one area of the receptive field are dynamically adjusted to the history of stimulation both within and outside that area. PMID:16191636
Features of flow around the flying wing model at various attack and slip angle
NASA Astrophysics Data System (ADS)
Pavlenko, A. M.; Zanin, B. Yu.; Katasonov, M. M.
2017-10-01
Experimental study of flow features around aircraft model having "flying wing" form and belonging to the category of small-unmanned aerial vehicleswas carried out. Hot-wire anemometry and flow visualization techniques were used in the investigation to get quantitative data and streamlines pictures ofthe flow near the model surface. Evolution of vortex structures depending on the attack and slip angle was demonstrated. The possibility of flow control and reduction of flow separation zones on the wing surface by means of ledges in the form of cones was also investigated. It was shown, that the laminar-turbulent transition scenario on the flying wing model is identical to the one on a straight wing and occurs through the development of a package of unstable oscillations in the boundary layer separation.
Controlling free flight of a robotic fly using an onboard vision sensor inspired by insect ocelli
Fuller, Sawyer B.; Karpelson, Michael; Censi, Andrea; Ma, Kevin Y.; Wood, Robert J.
2014-01-01
Scaling a flying robot down to the size of a fly or bee requires advances in manufacturing, sensing and control, and will provide insights into mechanisms used by their biological counterparts. Controlled flight at this scale has previously required external cameras to provide the feedback to regulate the continuous corrective manoeuvres necessary to keep the unstable robot from tumbling. One stabilization mechanism used by flying insects may be to sense the horizon or Sun using the ocelli, a set of three light sensors distinct from the compound eyes. Here, we present an ocelli-inspired visual sensor and use it to stabilize a fly-sized robot. We propose a feedback controller that applies torque in proportion to the angular velocity of the source of light estimated by the ocelli. We demonstrate theoretically and empirically that this is sufficient to stabilize the robot's upright orientation. This constitutes the first known use of onboard sensors at this scale. Dipteran flies use halteres to provide gyroscopic velocity feedback, but it is unknown how other insects such as honeybees stabilize flight without these sensory organs. Our results, using a vehicle of similar size and dynamics to the honeybee, suggest how the ocelli could serve this role. PMID:24942846
A New Flying Wire System for the Tevatron
NASA Astrophysics Data System (ADS)
Blokland, Willem; Dey, Joseph; Vogel, Greg
1997-05-01
A new Flying Wires system replaces the old system to enhance the analysis of the beam emittance, improve the reliability, and handle the upcoming upgrades of the Tevatron. New VME data acquisition modules and timing modules allow for more bunches to be sampled more precisely. The programming language LabVIEW, running on a Macintosh computer, controls the VME modules and the nuLogic motion board that flies the wires. LabVIEW also analyzes and stores the data, and handles local and remote commands. The new system flies three wires and fits profiles of 72 bunches to a gaussian function within two seconds. A new console application operates the flying wires from any control console. This paper discusses the hardware and software setup, the capabilities and measurement results of the new Flying Wires system.
Intravitreal Phacoemulsification Using Torsional Handpiece for Retained Lens Fragments.
Kumar, Vinod; Takkar, Brijesh
2016-01-01
To evaluate the results of intravitreal phacoemulsification with torsional hand piece in eyes with posteriorly dislocated lens fragments. In this prospective, interventional case series, 15 eyes with retained lens fragments following phacoemulsification were included. All patients underwent standard three-port pars plana vitrectomy and intravitreal phacoemulsification using sleeveless, torsional hand piece (OZiL™, Alcon's Infiniti Vision System). Patients were followed up for a minimum of six months to evaluate the visual outcomes and complications. The preoperative best-corrected visual acuity (BCVA) ranged from light perception to 0.3. No complications such as thermal burns of the scleral wound, retinal damage due to flying lens fragments, or difficult lens aspiration occurred during intravitreal phacoemulsification. Mean post-operative BCVA at the final follow-up was 0.5. Two eyes developed cystoid macular edema, which was managed medically. No retinal detachment was noted. Intravitreal phacoemulsification using torsional hand piece is a safe and effective alternative to conventional longitudinal phacofragmentation.
Unsupervised real-time speaker identification for daily movies
NASA Astrophysics Data System (ADS)
Li, Ying; Kuo, C.-C. Jay
2002-07-01
The problem of identifying speakers for movie content analysis is addressed in this paper. While most previous work on speaker identification was carried out in a supervised mode using pure audio data, more robust results can be obtained in real-time by integrating knowledge from multiple media sources in an unsupervised mode. In this work, both audio and visual cues will be employed and subsequently combined in a probabilistic framework to identify speakers. Particularly, audio information is used to identify speakers with a maximum likelihood (ML)-based approach while visual information is adopted to distinguish speakers by detecting and recognizing their talking faces based on face detection/recognition and mouth tracking techniques. Moreover, to accommodate for speakers' acoustic variations along time, we update their models on the fly by adapting to their newly contributed speech data. Encouraging results have been achieved through extensive experiments, which shows a promising future of the proposed audiovisual-based unsupervised speaker identification system.
[Development of spatial orientation during pilot training].
Ivanov, V V; Vorob'ev, O A; Snipkov, Iu Iu
1988-01-01
The problem of spatial orientation of pilots flying high-altitude aircraft is in the focus of present-day aviation medicine because of a growing number of accidents in the air. One of the productive lines of research is to study spatial orientation in terms of active formation and maintenance of its imagery in a complex environment. However investigators usually emphasize the role of visual (instrumental) information in the image construction, almost ignoring the sensorimotor component of spatial orientation. The theoretical analysis of the process of spatial orientation has facilitated the development of the concept assuming that the pattern of space perception changes with growing professional experience. The concept is based on an active approach to the essence, emergence, formation and variation in the pattern of sensory perception of space in man's consciousness. This concept asserts that as pilot's professional expertise increases, the pattern of spatial orientation becomes geocentric because a new system of spatial perception evolves which is a result of the development of a new (instrumental) type of motor activity in space. This finds expression in the fact that perception of spatial position inflight occurs when man has to resolve a new motor task--movement along a complex trajectory in the three-dimensional space onboard a flying vehicle. The meaningful structure of this problem which is to be implemented through controlling movements of the pilot acts as a factor that forms this new system of perception. All this underlies the arrangement of meaningful collection of instrumental data and detection of noninstrumental signals in the comprehensive perception of changes in the spatial position of a flying vehicle.
A Novel Treatment of Fear of Flying Using a Large Virtual Reality System.
Czerniak, Efrat; Caspi, Asaf; Litvin, Michal; Amiaz, Revital; Bahat, Yotam; Baransi, Hani; Sharon, Hanania; Noy, Shlomo; Plotnik, Meir
2016-04-01
Fear of flying (FoF), a common phobia in the developed world, is usually treated with cognitive behavioral therapy, most efficiently when combined with exposure methods, e.g., virtual reality exposure therapy (VRET). We evaluated FoF treatment using VRET in a large motion-based VR system. The treated subjects were seated on a moving platform. The virtual scenery included the interior of an aircraft and a window view to the outside world accompanied by platform movements simulating, e.g., takeoff, landing, and air turbulence. Relevant auditory stimuli were also incorporated. Three male patients with FoF underwent a clinical interview followed by three VRETs in the presence and with the guidance of a therapist. Scores on the Flight Anxiety Situation (FAS) and Flight Anxiety Modality (FAM) questionnaires were obtained on the first and fourth visits. Anxiety levels were assessed using the subjective units of distress (SUDs) scale during the exposure. All three subjects expressed satisfaction regarding the procedure and did not skip or avoid any of its stages. Consistent improvement was seen in the SUDs throughout the VRET session and across sessions, while patients' scores on the FAS and FAM showed inconsistent trends. Two patients participated in actual flights in the months following the treatment, bringing 12 and 16 yr of avoidance to an end. This VR-based treatment includes critical elements for exposure of flying experience beyond visual and auditory stimuli. The current case reports suggest VRET sessions may have a meaningful impact on anxiety levels, yet additional research seems warranted.
A Novel Method for Tracking Individuals of Fruit Fly Swarms Flying in a Laboratory Flight Arena.
Cheng, Xi En; Qian, Zhi-Ming; Wang, Shuo Hong; Jiang, Nan; Guo, Aike; Chen, Yan Qiu
2015-01-01
The growing interest in studying social behaviours of swarming fruit flies, Drosophila melanogaster, has heightened the need for developing tools that provide quantitative motion data. To achieve such a goal, multi-camera three-dimensional tracking technology is the key experimental gateway. We have developed a novel tracking system for tracking hundreds of fruit flies flying in a confined cubic flight arena. In addition to the proposed tracking algorithm, this work offers additional contributions in three aspects: body detection, orientation estimation, and data validation. To demonstrate the opportunities that the proposed system offers for generating high-throughput quantitative motion data, we conducted experiments on five experimental configurations. We also performed quantitative analysis on the kinematics and the spatial structure and the motion patterns of fruit fly swarms. We found that there exists an asymptotic distance between fruit flies in swarms as the population density increases. Further, we discovered the evidence for repulsive response when the distance between fruit flies approached the asymptotic distance. Overall, the proposed tracking system presents a powerful method for studying flight behaviours of fruit flies in a three-dimensional environment.
NASA Astrophysics Data System (ADS)
Arthur, Jarvis J., III; Prinzel, Lawrence J., III; Williams, Steven P.; Bailey, Randall E.; Shelton, Kevin J.; Norman, R. Mike
2011-06-01
NASA is researching innovative technologies for the Next Generation Air Transportation System (NextGen) to provide a "Better-Than-Visual" (BTV) capability as adjunct to "Equivalent Visual Operations" (EVO); that is, airport throughputs equivalent to that normally achieved during Visual Flight Rules (VFR) operations rates with equivalent and better safety in all weather and visibility conditions including Instrument Meteorological Conditions (IMC). These new technologies build on proven flight deck systems and leverage synthetic and enhanced vision systems. Two piloted simulation studies were conducted to access the use of a Head-Worn Display (HWD) with head tracking for synthetic and enhanced vision systems concepts. The first experiment evaluated the use a HWD for equivalent visual operations to San Francisco International Airport (airport identifier: KSFO) compared to a visual concept and a head-down display concept. A second experiment evaluated symbology variations under different visibility conditions using a HWD during taxi operations at Chicago O'Hare airport (airport identifier: KORD). Two experiments were conducted, one in a simulated San Francisco airport (KSFO) approach operation and the other, in simulated Chicago O'Hare surface operations, evaluating enhanced/synthetic vision and head-worn display technologies for NextGen operations. While flying a closely-spaced parallel approach to KSFO, pilots rated the HWD, under low-visibility conditions, equivalent to the out-the-window condition, under unlimited visibility, in terms of situational awareness (SA) and mental workload compared to a head-down enhanced vision system. There were no differences between the 3 display concepts in terms of traffic spacing and distance and the pilot decision-making to land or go-around. For the KORD experiment, the visibility condition was not a factor in pilot's rating of clutter effects from symbology. Several concepts for enhanced implementations of an unlimited field-of-regard BTV concept for low-visibility surface operations were determined to be equivalent in pilot ratings of efficacy and usability.
NASA Technical Reports Server (NTRS)
Arthur, Jarvis J., III; Prinzell, Lawrence J.; Williams, Steven P.; Bailey, Randall E.; Shelton, Kevin J.; Norman, R. Mike
2011-01-01
NASA is researching innovative technologies for the Next Generation Air Transportation System (NextGen) to provide a "Better-Than-Visual" (BTV) capability as adjunct to "Equivalent Visual Operations" (EVO); that is, airport throughputs equivalent to that normally achieved during Visual Flight Rules (VFR) operations rates with equivalent and better safety in all weather and visibility conditions including Instrument Meteorological Conditions (IMC). These new technologies build on proven flight deck systems and leverage synthetic and enhanced vision systems. Two piloted simulation studies were conducted to access the use of a Head-Worn Display (HWD) with head tracking for synthetic and enhanced vision systems concepts. The first experiment evaluated the use a HWD for equivalent visual operations to San Francisco International Airport (airport identifier: KSFO) compared to a visual concept and a head-down display concept. A second experiment evaluated symbology variations under different visibility conditions using a HWD during taxi operations at Chicago O'Hare airport (airport identifier: KORD). Two experiments were conducted, one in a simulated San Francisco airport (KSFO) approach operation and the other, in simulated Chicago O'Hare surface operations, evaluating enhanced/synthetic vision and head-worn display technologies for NextGen operations. While flying a closely-spaced parallel approach to KSFO, pilots rated the HWD, under low-visibility conditions, equivalent to the out-the-window condition, under unlimited visibility, in terms of situational awareness (SA) and mental workload compared to a head-down enhanced vision system. There were no differences between the 3 display concepts in terms of traffic spacing and distance and the pilot decision-making to land or go-around. For the KORD experiment, the visibility condition was not a factor in pilot's rating of clutter effects from symbology. Several concepts for enhanced implementations of an unlimited field-of-regard BTV concept for low-visibility surface operations were determined to be equivalent in pilot ratings of efficacy and usability.
McAlinden, Colm; Skiadaresi, Eirini; Moore, Jonathan E
2011-05-01
To investigate the visual and refractive outcomes following laser-assisted subepithelial keratectomy (LASEK) surgery with a flying-spot excimer laser. Private practice, Ireland. Case series. In this prospective study, the mean manifest spherical equivalent (SE), sphere, and cylinder were measured preoperatively. All eyes had LASEK surgery with an aberration-free algorithm with the Schwind Amaris excimer laser. Outcomes measured at 1 month, 6 months, and 1 year were uncorrected distance visual acuity (UDVA), manifest refraction, corrected distance visual acuity, contrast sensitivity, aberrometry, and complications. Accuracy, efficacy, and safety were evaluated at 1 year. Preoperatively, the mean SE, sphere, and cylinder in the 80 eyes (48 patients) were -3.58 diopters (D) ± 2.00 (SD), -3.23 ± 1.93 D, and -0.85 ± 0.65 D, respectively. One year postoperatively, the mean SE was -0.00 ± 0.22 D; 57 eyes (71%) were within -0.13 to +0.13 D of the SE, and 71 eyes (98%) were within ±0.50 D. The mean UDVA was -0.06 ± 0.07 logMAR, with an efficacy index of 1.04. The postoperative SE was stable between 1 month, 3 months, and 1 year. One eye (1%) had a change in SE by more than 0.50 D at 6 months and 1 year. There were no statistically significant differences in any aberrations at 1 year. The contrast sensitivity improved from 1.66 ± 0.17 log units preoperatively to 1.72 ± 0.15 log units at 1 month postoperatively (P=.0003), which was unchanged at 6 months and 1 year. This study demonstrated the effectiveness of LASEK for the treatment of myopia with this flying-spot excimer laser. Copyright © 2011 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.
Famous landmark identification in amnestic mild cognitive impairment and Alzheimer's disease.
Sheardova, Katerina; Laczó, Jan; Vyhnalek, Martin; Andel, Ross; Mokrisova, Ivana; Vlcek, Kamil; Amlerova, Jana; Hort, Jakub
2014-01-01
Identification of famous landmarks (FLI), famous faces (FFI) and recognition of facial emotions (FER) is affected early in the course of Alzheimer's disease (AD). FFI, FER and FLI may represent domain specific tasks relying on activation of distinct regions of the medial temporal lobe, which are affected successively during the course of AD. However, the data on FFI and FER in MCI are controversial and FLI domain remains almost unexplored. To determine whether and how are these three specific domains impaired in head to head comparison of patients with amnestic MCI (aMCI) single domain (SD-aMCI) and multiple domain (MD-aMCI). We propose that FLI might be most reliable in differentiating SD-aMCI, which is considered to be an earlier stage of AD pathology spread out, from the controls. A total of 114 patients, 13 with single domain (SD-aMCI) and 30 with multiple domains (MD-aMCI), 29 with mild AD and 42 controls underwent standard neurological and neuropsychological evaluations as well as tests of FLI, FER and FFI. Compared to the control group, AD subjects performed worse on FFI (p = 0.020), FER (p<0.001) and FLI (p<0.001), MD-aMCI group had significantly worse scores only on FLI (p = 0.002) and approached statistical significance on FER (0.053). SD-aMCI group performed significantly worse only on FLI (p = 0.028) compared to controls. Patients with SD-aMCI had an isolated impairment restricted to FLI, while patients with MD-aMCI showed impairment in FLI as well as in FER. Patients with mild dementia due to AD have more extensive impairment of higher visual perception. The results suggest that FLI testing may contribute to identification of patients at risk of AD. We hypothesize that clinical examination of all three domains might reflect the spread of the disease from transentorhinal cortex, over amygdala to fusiform gyrus.
Famous Landmark Identification in Amnestic Mild Cognitive Impairment and Alzheimer's Disease
Sheardova, Katerina; Laczó, Jan; Vyhnalek, Martin; Andel, Ross; Mokrisova, Ivana; Vlcek, Kamil; Amlerova, Jana; Hort, Jakub
2014-01-01
Background Identification of famous landmarks (FLI), famous faces (FFI) and recognition of facial emotions (FER) is affected early in the course of Alzheimer's disease (AD). FFI, FER and FLI may represent domain specific tasks relying on activation of distinct regions of the medial temporal lobe, which are affected successively during the course of AD. However, the data on FFI and FER in MCI are controversial and FLI domain remains almost unexplored. Objectives To determine whether and how are these three specific domains impaired in head to head comparison of patients with amnestic MCI (aMCI) single domain (SD-aMCI) and multiple domain (MD-aMCI). We propose that FLI might be most reliable in differentiating SD-aMCI, which is considered to be an earlier stage of AD pathology spread out, from the controls. Patients and Methods A total of 114 patients, 13 with single domain (SD–aMCI) and 30 with multiple domains (MD–aMCI), 29 with mild AD and 42 controls underwent standard neurological and neuropsychological evaluations as well as tests of FLI, FER and FFI. Results Compared to the control group, AD subjects performed worse on FFI (p = 0.020), FER (p<0.001) and FLI (p<0.001), MD-aMCI group had significantly worse scores only on FLI (p = 0.002) and approached statistical significance on FER (0.053). SD-aMCI group performed significantly worse only on FLI (p = 0.028) compared to controls. Conclusions Patients with SD-aMCI had an isolated impairment restricted to FLI, while patients with MD–aMCI showed impairment in FLI as well as in FER. Patients with mild dementia due to AD have more extensive impairment of higher visual perception. The results suggest that FLI testing may contribute to identification of patients at risk of AD. We hypothesize that clinical examination of all three domains might reflect the spread of the disease from transentorhinal cortex, over amygdala to fusiform gyrus. PMID:25144755
NASA Technical Reports Server (NTRS)
Sitterley, T. E.
1974-01-01
The effectivess of an improved static retraining method was evaluated for a simulated space vehicle approach and landing under instrument and visual flight conditions. Experienced pilots were trained and then tested after 4 months without flying to compare their performance using the improved method with three methods previously evaluated. Use of the improved static retraining method resulted in no practical or significant skill degradation and was found to be even more effective than methods using a dynamic presentation of visual cues. The results suggested that properly structured open loop methods of flight control task retraining are feasible.
Rutishauser, Ueli; Kotowicz, Andreas; Laurent, Gilles
2013-01-01
Brain activity often consists of interactions between internal—or on-going—and external—or sensory—activity streams, resulting in complex, distributed patterns of neural activity. Investigation of such interactions could benefit from closed-loop experimental protocols in which one stream can be controlled depending on the state of the other. We describe here methods to present rapid and precisely timed visual stimuli to awake animals, conditional on features of the animal’s on-going brain state; those features are the presence, power and phase of oscillations in local field potentials (LFP). The system can process up to 64 channels in real time. We quantified its performance using simulations, synthetic data and animal experiments (chronic recordings in the dorsal cortex of awake turtles). The delay from detection of an oscillation to the onset of a visual stimulus on an LCD screen was 47.5 ms and visual-stimulus onset could be locked to the phase of ongoing oscillations at any frequency ≤40 Hz. Our software’s architecture is flexible, allowing on-the-fly modifications by experimenters and the addition of new closed-loop control and analysis components through plugins. The source code of our system “StimOMatic” is available freely as open-source. PMID:23473800
Automated 3D trajectory measuring of large numbers of moving particles.
Wu, Hai Shan; Zhao, Qi; Zou, Danping; Chen, Yan Qiu
2011-04-11
Complex dynamics of natural particle systems, such as insect swarms, bird flocks, fish schools, has attracted great attention of scientists for years. Measuring 3D trajectory of each individual in a group is vital for quantitative study of their dynamic properties, yet such empirical data is rare mainly due to the challenges of maintaining the identities of large numbers of individuals with similar visual features and frequent occlusions. We here present an automatic and efficient algorithm to track 3D motion trajectories of large numbers of moving particles using two video cameras. Our method solves this problem by formulating it as three linear assignment problems (LAP). For each video sequence, the first LAP obtains 2D tracks of moving targets and is able to maintain target identities in the presence of occlusions; the second one matches the visually similar targets across two views via a novel technique named maximum epipolar co-motion length (MECL), which is not only able to effectively reduce matching ambiguity but also further diminish the influence of frequent occlusions; the last one links 3D track segments into complete trajectories via computing a globally optimal assignment based on temporal and kinematic cues. Experiment results on simulated particle swarms with various particle densities validated the accuracy and robustness of the proposed method. As real-world case, our method successfully acquired 3D flight paths of fruit fly (Drosophila melanogaster) group comprising hundreds of freely flying individuals. © 2011 Optical Society of America
A Simulation Study on Take-Off and Landing Dynamics of the Aircraft of a Fly-By-Wire Control System
1993-01-07
L:V,"DIN G DYN;AMICS OF THE AIRCRAFT OF A FLY-BY-WIRE CONTROL SYSTEM by Y achang Feng, Gang Chert, Peiqiong Li 93-00985 Distribution unlimit ed. FASTC...FLY-BY-WIRE CONTROL SYSTEM By: Yachang Feng, Gang Chen, Peiqiong- Li English pages: 17 Source: Hangkon, Xuebao, Vol. 12, No. 6, June, 1991; pp. 252-258...Landing Dynamics of the Aircraft of a Fly-By-Wire Control System Beijing University of Aeronautics and Astronautics Yachang FENG, Gang CHEN and Peiqiong Li
Assessing Impact of Dual Sensor Enhanced Flight Vision Systems on Departure Performance
NASA Technical Reports Server (NTRS)
Kramer, Lynda J.; Etherington, Timothy J.; Severance, Kurt; Bailey, Randall E.
2016-01-01
Synthetic Vision (SV) and Enhanced Flight Vision Systems (EFVS) may serve as game-changing technologies to meet the challenges of the Next Generation Air Transportation System and the envisioned Equivalent Visual Operations (EVO) concept - that is, the ability to achieve the safety and operational tempos of current-day Visual Flight Rules operations irrespective of the weather and visibility conditions. One significant obstacle lies in the definition of required equipage on the aircraft and on the airport to enable the EVO concept objective. A motion-base simulator experiment was conducted to evaluate the operational feasibility and pilot workload of conducting departures and approaches on runways without centerline lighting in visibility as low as 300 feet runway visual range (RVR) by use of onboard vision system technologies on a Head-Up Display (HUD) without need or reliance on natural vision. Twelve crews evaluated two methods of combining dual sensor (millimeter wave radar and forward looking infrared) EFVS imagery on pilot-flying and pilot-monitoring HUDs. In addition, the impact of adding SV to the dual sensor EFVS imagery on crew flight performance and workload was assessed. Using EFVS concepts during 300 RVR terminal operations on runways without centerline lighting appears feasible as all EFVS concepts had equivalent (or better) departure performance and landing rollout performance, without any workload penalty, than those flown with a conventional HUD to runways having centerline lighting. Adding SV imagery to EFVS concepts provided situation awareness improvements but no discernible improvements in flight path maintenance.
An Analysis of Helicopter Pilot Scan Techniques While Flying at Low Altitudes and High Speed
2012-09-01
Manager SV Synthetic Vision TFH Total Flight Hours TOFT Tactical Operational Flight Trainer VFR Visual Flight Rules VMC Visual Meteorological...Crognale, 2008). Recently, the use of synthetic vision (SV) and a heads-up- display (HUD) have been a topic of discussion in the aviation community... Synthetic vision uses external cameras to provide the pilot with an enhanced view of the outside world, usually with the assistance of night vision
NASA Astrophysics Data System (ADS)
Raphael, David T.; McIntee, Diane; Tsuruda, Jay S.; Colletti, Patrick; Tatevossian, Raymond; Frazier, James
2006-03-01
We explored multiple image processing approaches by which to display the segmented adult brachial plexus in a three-dimensional manner. Magnetic resonance neurography (MRN) 1.5-Tesla scans with STIR sequences, which preferentially highlight nerves, were performed in adult volunteers to generate high-resolution raw images. Using multiple software programs, the raw MRN images were then manipulated so as to achieve segmentation of plexus neurovascular structures, which were incorporated into three different visualization schemes: rotating upper thoracic girdle skeletal frames, dynamic fly-throughs parallel to the clavicle, and thin slab volume-rendered composite projections.
Arthofer, Wolfgang; Decristoforo, Clemens; Schlick-Steiner, Birgit C.; Steiner, Florian M.
2016-01-01
Knowledge of a species’ ecology, including its movement in time and space, is key for many questions in biology and conservation. While numerous tools for tracking larger animals are available, millimetre-sized insects are averse to standard tracking and labelling procedures. Here, we evaluated the applicability of ultra-low, permission-exempt activities of the metastable isomer of the radionuclide Technetium-99 for labelling and field detection of the mountain fly Drosophila nigrosparsa. We demonstrate that an activity of less than 10 MBq is sufficient to label dozens of flies and detect single individuals using standard radiation protection monitors. The methodology presented here is applicable to many small-sized, low-mobility animals as well as independent from light and weather conditions and visual contact with the target organism. PMID:27812000
Automated Surveillance of Fruit Flies
Potamitis, Ilyas; Rigakis, Iraklis; Tatlas, Nicolaos-Alexandros
2017-01-01
Insects of the Diptera order of the Tephritidae family cause costly, annual crop losses worldwide. Monitoring traps are important components of integrated pest management programs used against fruit flies. Here we report the modification of typical, low-cost plastic traps for fruit flies by adding the necessary optoelectronic sensors to monitor the entrance of the trap in order to detect, time-stamp, GPS tag, and identify the species of incoming insects from the optoacoustic spectrum analysis of their wingbeat. We propose that the incorporation of automated streaming of insect counts, environmental parameters and GPS coordinates into informative visualization of collective behavior will finally enable better decision making across spatial and temporal scales, as well as administrative levels. The device presented is at product level of maturity as it has solved many pending issues presented in a previously reported study. PMID:28075346
No evidence for visual context-dependency of olfactory learning in Drosophila
NASA Astrophysics Data System (ADS)
Yarali, Ayse; Mayerle, Moritz; Nawroth, Christian; Gerber, Bertram
2008-08-01
How is behaviour organised across sensory modalities? Specifically, we ask concerning the fruit fly Drosophila melanogaster how visual context affects olfactory learning and recall and whether information about visual context is getting integrated into olfactory memory. We find that changing visual context between training and test does not deteriorate olfactory memory scores, suggesting that these olfactory memories can drive behaviour despite a mismatch of visual context between training and test. Rather, both the establishment and the recall of olfactory memory are generally facilitated by light. In a follow-up experiment, we find no evidence for learning about combinations of odours and visual context as predictors for reinforcement even after explicit training in a so-called biconditional discrimination task. Thus, a ‘true’ interaction between visual and olfactory modalities is not evident; instead, light seems to influence olfactory learning and recall unspecifically, for example by altering motor activity, alertness or olfactory acuity.
DOT National Transportation Integrated Search
1967-08-01
Disorientation caused by 'Coriolis' vestibular reactions has been cited frequently as a significant factor in flying safety. In addition, personnel who maintain rotating radar towers may also be adversely affected by 'Coriolis' problems. In the study...
Direction selectivity of blowfly motion-sensitive neurons is computed in a two-stage process.
Borst, A; Egelhaaf, M
1990-01-01
Direction selectivity of motion-sensitive neurons is generally thought to result from the nonlinear interaction between the signals derived from adjacent image points. Modeling of motion-sensitive networks, however, reveals that such elements may still respond to motion in a rather poor directionally selective way. Direction selectivity can be significantly enhanced if the nonlinear interaction is followed by another processing stage in which the signals of elements with opposite preferred directions are subtracted from each other. Our electrophysiological experiments in the fly visual system suggest that here direction selectivity is acquired in such a two-stage process. Images PMID:2251278
Development of diet-induced insulin resistance in adult Drosophila melanogaster
Morris, Siti Nur Sarah; Coogan, Claire; Chamseddin, Khalil; Fernandez-Kim, Sun Ok; Kolli, Santharam; Keller, Jeffrey N.; Bauer, Johannes H.
2013-01-01
The fruit fly Drosophila melanogaster is increasingly utilized as an alternative to costly rodent models to study human diseases. Fly models exist for a wide variety of human conditions, such as Alzheimer's and Parkinson’s Disease, or cardiac function. Advantages of the fly system are its rapid generation time and its low cost. However, the greatest strength of the fly system are the powerful genetic tools that allow for rapid dissection of molecular disease mechanisms. Here, we describe the diet-dependent development of metabolic phenotypes in adult fruit flies. Depending on the specific type of nutrient, as well as its relative quantity in the diet, flies show weight gain and changes in the levels of storage macromolecules. Furthermore, the activity of insulin-signaling in the major metabolic organ of the fly, the fat body, decreases upon overfeeding. This decrease in insulin-signaling activity in overfed flies is moreover observed when flies are challenged with an acute food stimulus, suggesting that overfeeding leads to insulin resistance. Similar changes were observed in aging flies, with the development of the insulin resistance-like phenotype beginning at early middle ages. Taken together, these data demonstrate that imbalanced diet disrupts metabolic homeostasis in adult D. melanogaster and promotes insulin-resistant phenotypes. Therefore, the fly system may be a useful alternative tool in the investigation of molecular mechanisms of insulin resistance and the development of pharmacologic treatment options. PMID:22542511
NASA Astrophysics Data System (ADS)
Müller, Dietmar; Qin, Xiaodong; Sandwell, David; Dutkiewicz, Adriana; Williams, Simon; Flament, Nicolas; Maus, Stefan; Seton, Maria
2017-04-01
The pace of scientific discovery is being transformed by the availability of 'big data' and open access, open source software tools. These innovations open up new avenues for how scientists communicate and share data and ideas with each other, and with the general public. Here, we describe our efforts to bring to life our studies of the Earth system, both at present day and through deep geological time. The GPlates Portal (portal.gplates.org) is a gateway to a series of virtual globes based on the Cesium Javascript library. The portal allows fast interactive visualization of global geophysical and geological data sets, draped over digital terrain models. The globes use WebGL for hardware-accelerated graphics and are cross-platform and cross-browser compatible with complete camera control. The globes include a visualization of a high-resolution global digital elevation model and the vertical gradient of the global gravity field, highlighting small-scale seafloor fabric such as abyssal hills, fracture zones and seamounts in unprecedented detail. The portal also features globes portraying seafloor geology and a global data set of marine magnetic anomaly identifications. The portal is specifically designed to visualize models of the Earth through geological time. These space-time globes include tectonic reconstructions of the Earth's gravity and magnetic fields, and several models of long-wavelength surface dynamic topography through time, including the interactive plotting of vertical motion histories at selected locations. The portal has been visited over half a million times since its inception in October 2015, as tracked by google analytics, and the globes have been featured in numerous media articles around the world. This demonstrates the high demand for fast visualization of global spatial big data, both for the present-day as well as through geological time. The globes put the on-the-fly visualization of massive data sets at the fingertips of end-users to stimulate teaching and learning and novel avenues of inquiry. This technology offers many future opportunities for providing additional functionality, especially on-the-fly big data analytics. Müller, R.D., Qin, X., Sandwell, D.T., Dutkiewicz, A., Williams, S.E., Flament, N., Maus, S. and Seton, M, 2016, The GPlates Portal: Cloud-based interactive 3D visualization of global geophysical and geological data in a web browser, PLoS ONE 11(3): e0150883. doi:10.1371/ journal.pone.0150883
Minimum viewing angle for visually guided ground speed control in bumblebees.
Baird, Emily; Kornfeldt, Torill; Dacke, Marie
2010-05-01
To control flight, flying insects extract information from the pattern of visual motion generated during flight, known as optic flow. To regulate their ground speed, insects such as honeybees and Drosophila hold the rate of optic flow in the axial direction (front-to-back) constant. A consequence of this strategy is that its performance varies with the minimum viewing angle (the deviation from the frontal direction of the longitudinal axis of the insect) at which changes in axial optic flow are detected. The greater this angle, the later changes in the rate of optic flow, caused by changes in the density of the environment, will be detected. The aim of the present study is to examine the mechanisms of ground speed control in bumblebees and to identify the extent of the visual range over which optic flow for ground speed control is measured. Bumblebees were trained to fly through an experimental tunnel consisting of parallel vertical walls. Flights were recorded when (1) the distance between the tunnel walls was either 15 or 30 cm, (2) the visual texture on the tunnel walls provided either strong or weak optic flow cues and (3) the distance between the walls changed abruptly halfway along the tunnel's length. The results reveal that bumblebees regulate ground speed using optic flow cues and that changes in the rate of optic flow are detected at a minimum viewing angle of 23-30 deg., with a visual field that extends to approximately 155 deg. By measuring optic flow over a visual field that has a low minimum viewing angle, bumblebees are able to detect and respond to changes in the proximity of the environment well before they are encountered.
A Novel Method for Tracking Individuals of Fruit Fly Swarms Flying in a Laboratory Flight Arena
Cheng, Xi En; Qian, Zhi-Ming; Wang, Shuo Hong; Jiang, Nan; Guo, Aike; Chen, Yan Qiu
2015-01-01
The growing interest in studying social behaviours of swarming fruit flies, Drosophila melanogaster, has heightened the need for developing tools that provide quantitative motion data. To achieve such a goal, multi-camera three-dimensional tracking technology is the key experimental gateway. We have developed a novel tracking system for tracking hundreds of fruit flies flying in a confined cubic flight arena. In addition to the proposed tracking algorithm, this work offers additional contributions in three aspects: body detection, orientation estimation, and data validation. To demonstrate the opportunities that the proposed system offers for generating high-throughput quantitative motion data, we conducted experiments on five experimental configurations. We also performed quantitative analysis on the kinematics and the spatial structure and the motion patterns of fruit fly swarms. We found that there exists an asymptotic distance between fruit flies in swarms as the population density increases. Further, we discovered the evidence for repulsive response when the distance between fruit flies approached the asymptotic distance. Overall, the proposed tracking system presents a powerful method for studying flight behaviours of fruit flies in a three-dimensional environment. PMID:26083385
Gorresen, Paulo Marcos; Cryan, Paul; Montoya-Aiona, Kristina; Bonaccorso, Frank
2017-01-01
Bats vocalize during flight as part of the sensory modality called echolocation, but very little is known about whether flying bats consistently call. Occasional vocal silence during flight when bats approach prey or conspecifics has been documented for relatively few species and situations. Bats flying alone in clutter-free airspace are not known to forgo vocalization, yet prior observations suggested possible silent behavior in certain, unexpected situations. Determining when, why, and where silent behavior occurs in bats will help evaluate major assumptions of a primary monitoring method for bats used in ecological research, management, and conservation. In this study, we recorded flight activity of Hawaiian hoary bats (Lasiurus cinereus semotus) under seminatural conditions using both thermal video cameras and acoustic detectors. Simultaneous video and audio recordings from 20 nights of observation at 10 sites were analyzed for correspondence between detection methods, with a focus on video observations in three distance categories for which accompanying vocalizations were detected. Comparison of video and audio detections revealed that a high proportion of Hawaiian hoary bats “seen” on video were not simultaneously “heard.” On average, only about one in three visual detections within a night had an accompanying call detection, but this varied greatly among nights. Bats flying on curved flight paths and individuals nearer the cameras were more likely to be detected by both methods. Feeding and social calls were detected, but no clear pattern emerged from the small number of observations involving closely interacting bats. These results may indicate that flying Hawaiian hoary bats often forgo echolocation, or do not always vocalize in a way that is detectable with common sampling and monitoring methods. Possible reasons for the low correspondence between visual and acoustic detections range from methodological to biological and include a number of biases associated with the propagation and detection of sound, cryptic foraging strategies, or conspecific presence. Silent flight behavior may be more prevalent in echolocating bats than previously appreciated, has profound implications for ecological research, and deserves further characterization and study.
Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera.
Søvik, Eirik; Plath, Jenny A; Devaud, Jean-Marc; Barron, Andrew B
2016-11-26
Honey bees demonstrate astonishing learning abilities and advanced social behavior and communication. In addition, their brain is small, easy to visualize and to study. Therefore, bees have long been a favored model amongst neurobiologists and neuroethologists for studying the neural basis of social and natural behavior. It is important, however, that the experimental techniques used to study bees do not interfere with the behaviors being studied. Because of this, it has been necessary to develop a range of techniques for pharmacological manipulation of honey bees. In this paper we demonstrate methods for treating restrained or free-flying honey bees with a wide range of pharmacological agents. These include both noninvasive methods such as oral and topical treatments, as well as more invasive methods that allow for precise drug delivery in either systemic or localized fashion. Finally, we discuss the advantages and disadvantages of each method and describe common hurdles and how to best overcome them. We conclude with a discussion on the importance of adapting the experimental method to the biological questions rather than the other way around.
Vislobokov, Nikolay A; Galinskaya, Tatiana V; Degtjareva, Galina V; Valiejo-Roman, Carmen M; Samigullin, Tahir H; Kuznetsov, Andrey N; Sokoloff, Dmitry D
2014-09-01
• Aspidistra is a species-rich, herbaceous monocot genus of tropical Southeast Asia. Most species are recently discovered and apparently endangered, though virtually nothing is known about their biology. Species of the genus are primarily distinguished using flower morphology, which is enormously diverse. However, the pollination process has not been directly observed in the center of diversity of the genus (N Vietnam and S China). Indirect and partly direct data on the only widely cultivated species of the genus (A. elatior) placed it among angiosperms with the most unusual pollination biology, though these data are highly controversial, suggesting pollen transfer by mollusks, crustaceans, flies, or possibly tiny soil invertebrates such as collembolans.• Pollination of Aspidistra xuansonensis in the center of diversity of the genus was studied using visual observations and videos and light and scanning electron microscopy investigation of flowers and their pollinators. Pollinators and their larvae were molecularly barcoded.• Aspidistra xuansonensis is pollinated by female cecidomyiid flies (gall midges). They oviposit on anthers, and larvae develop among the pollen mass. Molecular barcoding proved taxonomic identity of the larvae and the flies. The larvae neither damage floral parts nor cause gall formation, but feed on pollen grains by sucking out their content. The larvae move out of the flowers before decomposition starts. Carebara ants steal developing larvae from flowers but do not contribute to pollination.• More than one kind of myiophily is present in Aspidistra. Brood site pollination was documented for the first time in Aspidistra. The pollination system of A. xuansonensis differs from other kinds of brood site pollination in the exit of the larvae prior to the decomposition of floral parts. © 2014 Botanical Society of America, Inc.
NASA Technical Reports Server (NTRS)
Poehler, H. A.
1978-01-01
Results of a test of the use of a Lightning Detection and Ranging (LDAR) remote display in the Patrick AFB RAPCON facility are presented. Agreement between LDAR and radar precipitation echoes of the RAPCON radar was observed, as well as agreement between LDAR and pilot's visual observations of lightning flashes. A more precise comparison between LDAR and KSC based radars is achieved by the superposition of LDAR precipitation echoes. Airborne measurements of updrafts and turbulence by an armored T-28 aircraft flying through the thunderclouds are correlated with LDAR along the flight path. Calibration and measurements of the accuracy of the LDAR System are discussed, and the extended range of the system is illustrated.
FliO Regulation of FliP in the Formation of the Salmonella enterica Flagellum
Barker, Clive S.; Meshcheryakova, Irina V.; Kostyukova, Alla S.; Samatey, Fadel A.
2010-01-01
The type III secretion system of the Salmonella flagellum consists of 6 integral membrane proteins: FlhA, FlhB, FliO, FliP, FliQ, and FliR. However, in some other type III secretion systems, a homologue of FliO is apparently absent, suggesting it has a specialized role. Deleting the fliO gene from the chromosome of a motile strain of Salmonella resulted in a drastic decrease of motility. Incubation of the ΔfliO mutant strain in motility agar, gave rise to pseudorevertants containing extragenic bypass mutations in FliP at positions R143H or F190L. Using membrane topology prediction programs, and alkaline phosphatase or GFPuv chimeric protein fusions into the FliO protein, we demonstrated that FliO is bitopic with its N-terminus in the periplasm and C-terminus in the cytoplasm. Truncation analysis of FliO demonstrated that overexpression of FliO43–125 or FliO1–95 was able to rescue motility of the ΔfliO mutant. Further, residue leucine 91 in the cytoplasmic domain was identified to be important for function. Based on secondary structure prediction, the cytoplasmic domain, FliO43–125, should contain beta-structure and alpha-helices. FliO43–125-Ala was purified and studied using circular dichroism spectroscopy; however, this domain was disordered, and its structure was a mixture of beta-sheet and random coil. Coexpression of full-length FliO with FliP increased expression levels of FliP, but coexpression with the cytoplasmic domain of FliO did not enhance FliP expression levels. Overexpression of the cytoplasmic domain of FliO further rescued motility of strains deleted for the fliO gene expressing bypass mutations in FliP. These results suggest FliO maintains FliP stability through transmembrane domain interaction. The results also demonstrate that the cytoplasmic domain of FliO has functionality, and it presumably becomes structured while interacting with its binding partners. PMID:20941389
Dissection of Drosophila Visual Circuits Implicative in Figure Motion
NASA Astrophysics Data System (ADS)
Kelley, Ross G.
The Drosophila visual system offers a model to study the foundations of how motion signals are computed from raw visual input and transformed into behavioral output. My studies focus on how specific cells in the Drosophila nervous system implement this input-output transformation. The individual cell types are known from classical studies using Golgi impregnations, but the assembly of motion processing circuits and the behavioral outputs remain poorly understood. Using an electronic flight simulator for flies and a white-noise analysis developed by Aptekar et al., I screen specific neurons in the optic lobes for behavioral ramifications. This approach produces wing responses to both the spatial and temporal dynamics of motion signals. The results of these experiments give Spatiotemporal Action Fields (STAFs) across the entire visual panorama. Genetically inactivating a distinct grouping of cells in the third optic ganglion, the Lobula Plate, the Horizontal System (HS) cell group, produced a robust phenotype through STAF analysis. Using the Gal4-UAS transgene expression system, we selectively inactivated the HS cells by expressing in their membrane inward rectifying potassium channels (Kir2.1) to hyperpolarize these cells, preventing their role in synaptic signaling. The results of the experiments show mutants lose steering responses to several distinct categories of figure motion and reduced behavioral responses to figure motion set against a contrasting moving background, highlighting their role in figure tracking behavior. Finally, a synapse inactivating protein, tetanus toxin (TNT), expressed in the HS cell group, produces a different behavioral phenotype than overexpressing inward rectifier. TNT, a bacterial neurotoxin, cleaves SNARE proteins resulting in loss of synaptic output of the cell, but the dendrites are intact and signal normally, preserving dendro-dendritic interactions known to sculpt the visual receptive fields of these cells. The two distinct phenotypes to each genetically targeted silencer differentiate the functional role of dendritic integration versus axonal output in this important cell group.
Catching fly balls in virtual reality: a critical test of the outfielder problem
Fink, Philip W.; Foo, Patrick S.; Warren, William H.
2013-01-01
How does a baseball outfielder know where to run to catch a fly ball? The “outfielder problem” remains unresolved, and its solution would provide a window into the visual control of action. It may seem obvious that human action is based on an internal model of the physical world, such that the fielder predicts the landing point based on a mental model of the ball’s trajectory (TP). But two alternative theories, Optical Acceleration Cancellation (OAC) and Linear Optical Trajectory (LOT), propose that fielders are led to the right place at the right time by coupling their movements to visual information in a continuous “online” manner. All three theories predict successful catches and similar running paths. We provide a critical test by using virtual reality to perturb the vertical motion of the ball in mid-flight. The results confirm the predictions of OAC, but are at odds with LOT and TP. PMID:20055547
Mappes, Martina; Homberg, Uwe
2007-01-01
Many insects can detect the polarization pattern of the blue sky and rely on polarization vision for sky compass orientation. In laboratory experiments, tethered flying locusts perform periodic changes in flight behavior under a slowly rotating polarizer even if one eye is painted black. Anatomical tracing studies and intracellular recordings have suggested that the polarization vision pathway in the locust brain involves the anterior optic tract and tubercle, the lateral accessory lobe, and the central complex of the brain. To investigate whether visual pathways through the anterior optic tract mediate polarotaxis in the desert locust, we transected the tract on one side and tested polarotaxis (1) with both eyes unoccluded and (2) with the eye of the intact hemisphere painted black. In the second group of animals, but not in the first group, polarotaxis was abolished. Sham operations did not impair polarotaxis. The experiments show that the anterior optic tract is an indispensable part of visual pathways mediating polarotaxis in the desert locust.
Van De Poll, Matthew N; Zajaczkowski, Esmi L; Taylor, Gavin J; Srinivasan, Mandyam V; van Swinderen, Bruno
2015-11-01
Closed-loop paradigms provide an effective approach for studying visual choice behaviour and attention in small animals. Different flying and walking paradigms have been developed to investigate behavioural and neuronal responses to competing stimuli in insects such as bees and flies. However, the variety of stimulus choices that can be presented over one experiment is often limited. Current choice paradigms are mostly constrained as single binary choice scenarios that are influenced by the linear structure of classical conditioning paradigms. Here, we present a novel behavioural choice paradigm that allows animals to explore a closed geometry of interconnected binary choices by repeatedly selecting among competing objects, thereby revealing stimulus preferences in an historical context. We used our novel paradigm to investigate visual flicker preferences in honeybees (Apis mellifera) and found significant preferences for 20-25 Hz flicker and avoidance of higher (50-100 Hz) and lower (2-4 Hz) flicker frequencies. Similar results were found when bees were presented with three simultaneous choices instead of two, and when they were given the chance to select previously rejected choices. Our results show that honeybees can discriminate among different flicker frequencies and that their visual preferences are persistent even under different experimental conditions. Interestingly, avoided stimuli were more attractive if they were novel, suggesting that novelty salience can override innate preferences. Our recursive virtual reality environment provides a new approach to studying visual discrimination and choice behaviour in animals. © 2015. Published by The Company of Biologists Ltd.
Flight directions of passerine migrants in daylight and darkness: A radar and direct visual study
NASA Technical Reports Server (NTRS)
Gauthreaux, S. A., Jr.
1972-01-01
The application of radar and visual techniques to determine the migratory habits of passerine birds during daylight and darkness is discussed. The effects of wind on the direction of migration are examined. Scatter diagrams of daytime and nocturnal migration track directions correlated with wind direction are presented. It is concluded that migratory birds will fly at altitudes where wind direction and migratory direction are nearly the same. The effects of cloud cover and solar obscuration are considered negligible.
Smallegange, Renate C; Kelling, Frits J; Den Otter, Cornelis J
2008-12-01
Houseflies, Musca domestica, obtained from a high-larval-density culture were significantly (ca. 1.5 times) smaller than those from a low-larval-density culture. The same held true for their antennae and maxillary palps. Structure, number, and distribution of sensilla on antennae and palps of small and large flies were investigated using Scanning electron microscopy and Transmission electron microscopy. In each funiculus three pits were present, two (Type I) consisting of several compartments and one (Type II) of one compartment. Four types of olfactory sensilla were detected: trichoid sensilla on the funiculi, basiconic sensilla on funiculi and palps, grooved sensilla on funiculi and in pits Type I, and clavate sensilla on funiculi and in pits Type II. Type I pits also contained striated sensilla (presumably hygroreceptors). Mechanosensory bristles were present on scapes, pedicels, and palps. Noninnervated microtrichia were found on the palps and all antennal segments. The large houseflies possessed nearly twice as much sensilla as the small flies. So far, we did not observe differences in behavior between small and large flies. We assumed that small flies, being olfactory less equipped than large flies, may be able to compensate for this by, e.g., visual cues or by their olfactory sensilla being more sensitive than those of large flies. To be able to answer these questions careful studies have to be done on the behavioral responses of small and large flies to environmental stimuli. In addition, electrophysiological studies should be performed to reveal whether the responses of individual sensilla of flies reared under different conditions have been changed. 2008 Wiley-Liss, Inc.
Ecological study on antimicrobial-resistant zoonotic bacteria transmitted by flies in cattle farms.
Mohammed, Asmaa N; Abdel-Latef, Gihan K; Abdel-Azeem, Naglaa M; El-Dakhly, Khaled Mohamed
2016-10-01
Flies were qualitatively and quantitatively monitored on both livestock animals and the surrounding environment to investigate their role as a potential carrier for antimicrobial-resistant bacteria of zoonotic importance in cattle farms. This was done by the use of visual observations and animal photography; meanwhile, in the surrounding environment, flies were collected using sticky cards and then microscopically identified. Representative fly samples were cultured for bacterial isolation, biochemical identification, and then tested against common 12 antibiotics. The total average of dipterous flies in examined farms was 400.42 ± 6.2. Culicoides biting midges were the most common existing species (70.01 %) followed by house flies, stable flies, and mosquitoes (18.31, 7.74, and 3.91 %, respectively) at X (2) = 9.0, P < 0.05. The most predominant bacterial isolates were Escherichia coli (22.6 %), Staphylococcus aureus and Enterobacter (17.3 % each), coagulase-negative Staphylococci (CNS) (14.7 %), Klebsiella sp. (8 %), Salmonella spp. (6.7 %), and Shigella spp. and Proteus spp. (6.7 % each). The tested bacterial isolates were resistant to variant antibiotics used. S. aureus exhibited 100 % resistance to colistine. However, E. coli revealed 92.9 and 78.6 % resistance against tetracycline and colistine, respectively. Both Salmonella spp. and Shigella spp. were 100 % resistant to penicillin, and Klebsiella sp. had 100 % resistance to tetracycline. In conclusion, Culicoides biting midges and house flies could be considered as a potential carrier for multi-drug-resistant bacteria of zoonotic importance. Furthermore, cows' environment has an essential role in propagation and wide spread of antimicrobial-resistant bacterial pathogens.
The impact of Fli1 deficiency on the pathogenesis of systemic sclerosis
Asano, Yoshihide; Bujor, Andreea M.; Trojanowska, Maria
2013-01-01
Systemic sclerosis (SSc) is an autoimmune inflammatory disease with unknown etiology characterized by microvascular injury and fibrosis of the skin and internal organs. A growing body of evidence suggests that deficiency of the transcription factor Fli1 (Friend leukemia integration-1) has a pivotal role in the pathogenesis of SSc. Fli1 is expressed in fibroblasts, endothelial cells, and immune cells, and has important roles in the activation, differentiation, development, and survival of these cells. Previous studies demonstrated that Fli1 is downregulated in SSc fibroblasts by an epigenetic mechanism and a series of experiments with Fli1-deficient animal models revealed that Fli1 deficiency in fibroblasts and endothelial cells reproduces the histopathologic features of fibrosis and vasculopathy in SSc, respectively. In this article, we review the impact of Fli1 deficiency on the pathogenesis of SSc and discuss a new therapeutic strategy for SSc by targeting the transcription factor Fli1. PMID:20663647
Activating neurons by light in free-moving adult flies
NASA Astrophysics Data System (ADS)
Wu, Ming-Chin; Hsiao, Po-Yen; Chu, Li-An; Lin, Yen-Yin; Fu, Chien-Chung; Chiang, Ann-Shyn
2015-01-01
In this presentation, we show our preliminary results which is related to neurons activation in vivo by laser. A laser scanning system was adopted to guide laser beam to an assigned fly and an assigned position. A 473-nm laser can be a heat punishment source to restrain a wild-type fly's moving area. Furthermore, neurons in optogenetics transgene flies can be triggered by the blue laser in this system.
Weir, Peter T; Henze, Miriam J; Bleul, Christiane; Baumann-Klausener, Franziska; Labhart, Thomas; Dickinson, Michael H
2016-05-11
Many insects exploit skylight polarization as a compass cue for orientation and navigation. In the fruit fly, Drosophila melanogaster, photoreceptors R7 and R8 in the dorsal rim area (DRA) of the compound eye are specialized to detect the electric vector (e-vector) of linearly polarized light. These photoreceptors are arranged in stacked pairs with identical fields of view and spectral sensitivities, but mutually orthogonal microvillar orientations. As in larger flies, we found that the microvillar orientation of the distal photoreceptor R7 changes in a fan-like fashion along the DRA. This anatomical arrangement suggests that the DRA constitutes a detector for skylight polarization, in which different e-vectors maximally excite different positions in the array. To test our hypothesis, we measured responses to polarized light of varying e-vector angles in the terminals of R7/8 cells using genetically encoded calcium indicators. Our data confirm a progression of preferred e-vector angles from anterior to posterior in the DRA, and a strict orthogonality between the e-vector preferences of paired R7/8 cells. We observed decreased activity in photoreceptors in response to flashes of light polarized orthogonally to their preferred e-vector angle, suggesting reciprocal inhibition between photoreceptors in the same medullar column, which may serve to increase polarization contrast. Together, our results indicate that the polarization-vision system relies on a spatial map of preferred e-vector angles at the earliest stage of sensory processing. The fly's visual system is an influential model system for studying neural computation, and much is known about its anatomy, physiology, and development. The circuits underlying motion processing have received the most attention, but researchers are increasingly investigating other functions, such as color perception and object recognition. In this work, we investigate the early neural processing of a somewhat exotic sense, called polarization vision. Because skylight is polarized in an orientation that is rigidly determined by the position of the sun, this cue provides compass information. Behavioral experiments have shown that many species use the polarization pattern in the sky to direct locomotion. Here we describe the input stage of the fly's polarization-vision system. Copyright © 2016 the authors 0270-6474/16/365397-08$15.00/0.
Did a stellar fly-by shape the planetary system around Pr 0211 in the cluster M44?
NASA Astrophysics Data System (ADS)
Pfalzner, Susanne; Bhandare, Asmita; Vincke, Kirsten
2018-02-01
Out of the 3000 exoplanets detected so far, only 14 planets are members of open clusters: one of them is the exoplanet system around Pr 0211 in the cluster M44. The system consists of at least 2 planets, and the outer planet moves on a highly eccentric orbit at 5.5 AU. One hypothesis is that a close fly-by of a neighbouring star was responsible for the eccentric orbit. We test this hypothesis. First we determined the type of fly-by that would lead to the observed parameters, and then we used this result to determine the history of such fly-bys in simulations of the early dynamics in an M44-like environment. We find that although very close fly-bys are required to obtain the observed properties of Pr 0211c, such fly-bys are relatively common as a result of the high stellar density and longevity of the cluster. Such close fly-bys are most frequent during the first 1‑2 Myr after cluster formation, corresponding to a cluster age ≤3 Myr. During the first 2 to 3 Myr, about 6.5% of stars experience a fly-by that would lead to such a small system-size as observed for Pr 0211 or even smaller. It is unclear whether planets generally form on such short timescales. However, after this time, the close fly-by rate is still 0.2‑0.5 Myr‑1, which means that 12‑20% of stars would experience such close fly-bys over this time span when we extrapolate the situation to the age of M44. Our simulations show that the fly-by scenario is a realistic option for the formation of eccentricity orbits of the planets in M44 (Wang et al. 2015). The occurrence of such events is relatively high, leading to the expectation that similar systems are likely common in open clusters in general.
Development of diet-induced insulin resistance in adult Drosophila melanogaster.
Morris, Siti Nur Sarah; Coogan, Claire; Chamseddin, Khalil; Fernandez-Kim, Sun Ok; Kolli, Santharam; Keller, Jeffrey N; Bauer, Johannes H
2012-08-01
The fruit fly Drosophila melanogaster is increasingly utilized as an alternative to costly rodent models to study human diseases. Fly models exist for a wide variety of human conditions, such as Alzheimer's and Parkinson's Disease, or cardiac function. Advantages of the fly system are its rapid generation time and its low cost. However, the greatest strength of the fly system are the powerful genetic tools that allow for rapid dissection of molecular disease mechanisms. Here, we describe the diet-dependent development of metabolic phenotypes in adult fruit flies. Depending on the specific type of nutrient, as well as its relative quantity in the diet, flies show weight gain and changes in the levels of storage macromolecules. Furthermore, the activity of insulin-signaling in the major metabolic organ of the fly, the fat body, decreases upon overfeeding. This decrease in insulin-signaling activity in overfed flies is moreover observed when flies are challenged with an acute food stimulus, suggesting that overfeeding leads to insulin resistance. Similar changes were observed in aging flies, with the development of the insulin resistance-like phenotype beginning at early middle ages. Taken together, these data demonstrate that imbalanced diet disrupts metabolic homeostasis in adult D. melanogaster and promotes insulin-resistant phenotypes. Therefore, the fly system may be a useful alternative tool in the investigation of molecular mechanisms of insulin resistance and the development of pharmacologic treatment options. © 2012 Elsevier B.V. All rights reserved.
Interaction of feel system and flight control system dynamics on lateral flying qualities
NASA Technical Reports Server (NTRS)
Bailey, Randall E.; Powers, Bruce G.; Shafer, Mary F.
1988-01-01
An investigation of feel system and flight control system dynamics on lateral flying qualities was conducted using the variable stability USAF NT-33 aircraft. Experimental variations in feel system natural frequency, force-deflection gradient, control system command architecture type, flight control system filter frequency, and control system delay were made. The experiment data include pilot ratings using the Cooper-Harper (1969) rating scale, pilot comments, and tracking performance statistic. Three test pilots served as evaluators. The data indicate that as the feel system natural frequency is reduced lateral flying qualities degrade. At the slowest feel system frequency, the closed-loop response becomes nonlinear with a 'bobweight' effect apparent in the feel system. Feel system influences were essentially independent of the control system architecture. The flying qualities influence due to the feel system was different than when the identical dynamic systenm was used as a flight control system element.
IsoPlot: a database for comparison of mRNA isoforms in fruit fly and mosquitoes
Ng, I-Man; Tsai, Shang-Chi
2017-01-01
Abstract Alternative splicing (AS), a mechanism by which different forms of mature messenger RNAs (mRNAs) are generated from the same gene, widely occurs in the metazoan genomes. Knowledge about isoform variants and abundance is crucial for understanding the functional context in the molecular diversity of the species. With increasing transcriptome data of model and non-model species, a database for visualization and comparison of AS events with up-to-date information is needed for further research. IsoPlot is a publicly available database with visualization tools for exploration of AS events, including three major species of mosquitoes, Aedes aegypti, Anopheles gambiae, and Culex quinquefasciatus, and fruit fly Drosophila melanogaster, the model insect species. IsoPlot includes not only 88,663 annotated transcripts but also 17,037 newly predicted transcripts from massive transcriptome data at different developmental stages of mosquitoes. The web interface enables users to explore the patterns and abundance of isoforms in different experimental conditions as well as cross-species sequence comparison of orthologous transcripts. IsoPlot provides a platform for researchers to access comprehensive information about AS events in mosquitoes and fruit fly. Our database is available on the web via an interactive user interface with an intuitive graphical design, which is applicable for the comparison of complex isoforms within or between species. Database URL: http://isoplot.iis.sinica.edu.tw/ PMID:29220459
Voxel Datacubes for 3D Visualization in Blender
NASA Astrophysics Data System (ADS)
Gárate, Matías
2017-05-01
The growth of computational astrophysics and the complexity of multi-dimensional data sets evidences the need for new versatile visualization tools for both the analysis and presentation of the data. In this work, we show how to use the open-source software Blender as a three-dimensional (3D) visualization tool to study and visualize numerical simulation results, focusing on astrophysical hydrodynamic experiments. With a datacube as input, the software can generate a volume rendering of the 3D data, show the evolution of a simulation in time, and do a fly-around camera animation to highlight the points of interest. We explain the process to import simulation outputs into Blender using the voxel data format, and how to set up a visualization scene in the software interface. This method allows scientists to perform a complementary visual analysis of their data and display their results in an appealing way, both for outreach and science presentations.
Warren, Timothy L; Weir, Peter T; Dickinson, Michael H
2018-05-11
Animals must use external cues to maintain a straight course over long distances. In this study, we investigated how the fruit fly Drosophila melanogaster selects and maintains a flight heading relative to the axis of linearly polarized light, a visual cue produced by the atmospheric scattering of sunlight. To track flies' headings over extended periods, we used a flight simulator that coupled the angular velocity of dorsally presented polarized light to the stroke amplitude difference of the animals' wings. In the simulator, most flies actively maintained a stable heading relative to the axis of polarized light for the duration of 15 min flights. We found that individuals selected arbitrary, unpredictable headings relative to the polarization axis, which demonstrates that D . melanogaster can perform proportional navigation using a polarized light pattern. When flies flew in two consecutive bouts separated by a 5 min gap, the two flight headings were correlated, suggesting individuals retain a memory of their chosen heading. We found that adding a polarized light pattern to a light intensity gradient enhanced flies' orientation ability, suggesting D . melanogaster use a combination of cues to navigate. For both polarized light and intensity cues, flies' capacity to maintain a stable heading gradually increased over several minutes from the onset of flight. Our findings are consistent with a model in which each individual initially orients haphazardly but then settles on a heading which is maintained via a self-reinforcing process. This may be a general dispersal strategy for animals with no target destination. © 2018. Published by The Company of Biologists Ltd.
Brodie, Bekka S; Babcock, Tamara; Gries, Regine; Benn, Arlan; Gries, Gerhard
2016-01-01
We investigated foraging decisions by adult females of the common green bottle fly, Lucilia sericata, in accordance with their physiological state. When we gave female flies a choice between visually occluded, fresh canine feces (feeding site) and a CO2-euthanized rat (carrion oviposition site), 3-d-old "protein-starved" females responded equally well to feces and carrion, whereas protein-fed gravid females with mature oocytes responded only to carrion, indicating resource preferences based on a fly's physiological state. Dimethyl trisulfide (DMTS) is known to attract gravid L. sericata females to carrion. Therefore, we analyzed headspace from canine feces by gas chromatographic-electroantennographic detection (GC-EAD) and GC/mass spectrometry. In bioassays, of the 17 fecal odorants that elicited GC-EAD responses from fly antennae, a blend of indole and one or more of the alcohols phenol, m-/p-cresol and 1-octen-3-ol proved as attractive to flies as canine feces. Unlike young females, gravid females need to locate carrion for oviposition and distinguish between fresh and aging carrion, the latter possibly detrimental to offspring. Gravid female L. sericata accomplish this task, in part, by responding to trace amounts of DMTS emanating from fresh carrion and by discriminating against carrion as soon it begins to produce appreciable amounts of indole, which is also the second-most abundant semiochemical in fresh canine feces, and apparently serves as an indicator of food rather than oviposition resources. Our results emphasize the importance of studying foraging choices by flies in accordance with their physiological stage.
Commercial air travel after intraocular gas injection.
Houston, Stephen; Graf, Jürgen; Sharkey, James
2012-08-01
Passengers with intraocular gas are at risk of profound visual loss when exposed to reduced absolute pressure within the cabin of a typical commercial airliner. Information provided on the websites of the world's 10 largest airlines offer a considerable range of opinion as to when it might be safe to fly after gas injection. Physicians responsible for clearing pseassengers as 'fit to fly' should be aware modern retinal surgical techniques increasingly employ long-acting gases as vitreous substitutes. The kinetics of long-acting intraocular gases must be considered when deciding how long after surgery it is safe to travel. It is standard practice to advise passengers not to fly in aircraft until the gas is fully resorbed. To achieve this, it may be necessary to delay travel for approximately 2 wk after intraocular injection of sulfur hexafluoride (SF6) and for 6 wk after injection of perfluoropropane (C3F8).
Dual-modality smartphone endoscope for cervical pre-cancer detection (Conference Presentation)
NASA Astrophysics Data System (ADS)
Hong, Xiangqian; Yu, Bing
2017-02-01
Early detection is the key to the prevention of cervical cancer. There is an urgent need for a portable, affordable, and easy-to-use device for cervical pre-cancer detection, especially in low-resource settings. We have developed a dual-modality fiber-optic endoscope system (SmartME) that integrates high-resolution fluorescence imaging (FLI) and quantitative diffuse reflectance spectroscopy (DRS) onto a smartphone platform. The SmartME consists of a smartphone, a miniature fiber-optic endoscope, a phone attachment containing imaging optics, and a smartphone application (app). FLI is obtained by painting the tissue with a contrast agent (e.g., proflavine), illuminating the tissue and collecting its fluorescence images through an imaging bundle that is coupled to the phone camera. DRS is achieved by using a white LED, attaching additional source and detection fibers to the imaging bundle, and converting the phone camera into a spectrometer. The app collects images/spectra and transmits them to a remote server for analysis to extract the tissue parameters, including nuclear-to-cytoplasm ratio (calculated from FLI), concentrations of oxyhemoglobin (HbO2) and deoxyhemoglobin (Hb) as well as scattering (measured by DRS). These parameters can be used to detect cervical dysplasia. Our preliminary studies have demonstrated that the SmartME can clearly visualize the nuclei in living cells and in vivo biological samples, with a high spatial resolution of 3.1μm. The device can also measure tissue absorption and scattering properties with comparable accuracy to those of a benchtop DRS system. The SmartME has great potential to provide a compact, affordable, and `smart' solution for early detection of neoplastic changes in cervix.
A review of flight simulation techniques
NASA Astrophysics Data System (ADS)
Baarspul, Max
After a brief historical review of the evolution of flight simulation techniques, this paper first deals with the main areas of flight simulator applications. Next, it describes the main components of a piloted flight simulator. Because of the presence of the pilot-in-the-loop, the digital computer driving the simulator must solve the aircraft equations of motion in ‘real-time’. Solutions to meet the high required computer power of todays modern flight simulator are elaborated. The physical similarity between aircraft and simulator in cockpit layout, flight instruments, flying controls etc., is discussed, based on the equipment and environmental cue fidelity required for training and research simulators. Visual systems play an increasingly important role in piloted flight simulation. The visual systems now available and most widely used are described, where image generators and display devices will be distinguished. The characteristics of out-of-the-window visual simulation systems pertaining to the perceptual capabilities of human vision are discussed. Faithful reproduction of aircraft motion requires large travel, velocity and acceleration capabilities of the motion system. Different types and applications of motion systems in e.g. airline training and research are described. The principles of motion cue generation, based on the characteristics of the non-visual human motion sensors, are described. The complete motion system, consisting of the hardware and the motion drive software, is discussed. The principles of mathematical modelling of the aerodynamic, flight control, propulsion, landing gear and environmental characteristics of the aircraft are reviewed. An example of the identification of an aircraft mathematical model, based on flight and taxi tests, is presented. Finally, the paper deals with the hardware and software integration of the flight simulator components and the testing and acceptance of the complete flight simulator. Examples of the so-called ‘Computer Generated Checkout’ and ‘Proof of Match’ are presented. The concluding remarks briefly summarize the status of flight simulator technology and consider possibilities for future research.
Intelligent agents: adaptation of autonomous bimodal microsystems
NASA Astrophysics Data System (ADS)
Smith, Patrice; Terry, Theodore B.
2014-03-01
Autonomous bimodal microsystems exhibiting survivability behaviors and characteristics are able to adapt dynamically in any given environment. Equipped with a background blending exoskeleton it will have the capability to stealthily detect and observe a self-chosen viewing area while exercising some measurable form of selfpreservation by either flying or crawling away from a potential adversary. The robotic agent in this capacity activates a walk-fly algorithm, which uses a built in multi-sensor processing and navigation subsystem or algorithm for visual guidance and best walk-fly path trajectory to evade capture or annihilation. The research detailed in this paper describes the theoretical walk-fly algorithm, which broadens the scope of spatial and temporal learning, locomotion, and navigational performances based on optical flow signals necessary for flight dynamics and walking stabilities. By observing a fly's travel and avoidance behaviors; and, understanding the reverse bioengineering research efforts of others, we were able to conceptualize an algorithm, which works in conjunction with decisionmaking functions, sensory processing, and sensorimotor integration. Our findings suggest that this highly complex decentralized algorithm promotes inflight or terrain travel mobile stability which is highly suitable for nonaggressive micro platforms supporting search and rescue (SAR), and chemical and explosive detection (CED) purposes; a necessity in turbulent, non-violent structured or unstructured environments.
Limitations of learning in the proboscis reflex of the flower visiting syrphid fly Eristalis tenax
An, Lina; Donda, Miriam; Hohmann, Michele; Sermon, Leonie; Stegmanns, Vanessa
2018-01-01
Flower visiting Eristalis hoverflies feed on nectar and pollen and are known to rely on innate colour preferences. In addition to a preference for visiting yellow flowers, the flies possess an innate proboscis reflex elicited by chemical as well as yellow colour stimuli. In this study we show that the flies’ proboscis reflex is only triggered by yellow colour stimuli and not altered by conditioning to other colours. Neither in absolute nor in differential conditioning experiments the flies learned to associate other colours than yellow with reward. Even flies that experienced only blue nutrients during the first four days after hatching could not be trained to extend the proboscis towards other colours than yellow. The natural targets of the visually elicited proboscis reflex are yellow pollen and yellow anthers. One consequence of our findings is that flowers might advertise nectar and pollen rewards for Eristalis hoverflies by a yellow colour hue of nectar guides, nectaries, stamens or pollen. Alternatively, flowers might protect their pollen against Eristalis by displaying other pollen colours than yellow or direct flies by yellow pollen-mimicking floral guides towards nectar resources. Testing the proboscis extension of various hoverfly species in the field showed that only Eristalis hoverflies possess the proboscis reflex elicited by yellow colour hues. PMID:29558491
Holston, Kevin C.
2005-01-01
This study provides empirical evidence for habitat selection by North American species of stiletto flies (Diptera: Therevidae), based on local distributions of adults and immatures, and the first hypothesis of community assemblages proposed for a stiletto fly community. Sites at three localities within the Guadalupe-Nipomo dune system were sampled for stiletto flies in 1997 and 2001 by sifting sand, malaise trapping, and hand netting. Nine species were collected from four ecological zones and three intermediate ecological zones: Acrosathe novella (Coquillett), Brachylinga baccata (Loew), Nebritus powelli (Webb and Irwin), Ozodiceromyia sp., Pherocera sp., Tabudamima melanophleba (Loew), Thereva comata Loew, Thereva elizabethae Holston and Irwin, and Thereva fucata Loew. Species associations of adults and larvae with habitats and ecological zones were consistent among sites, suggesting that local distributions of coastal dune stiletto fly species are influenced by differences in habitat selection. In habitats dominated by the arroyo willow,Salix lasiolepsis, stiletto fly larvae of three species were collected in local sympatry, demonstrating that S. lasiolepsis stands along stabilized dune ridges can provide an intermediate ecological zone linking active dune and riparian habitat in the Guadalupe-Nipomo dune system. Sites dominated by European beach grass, Ammophilia arenaria, blue gum, Eucalyptus globulus, and Monterey cypress, Cupressus macrocarpa, are considered unsuitable for stiletto flies, which emphasizes the importance of terrestrial habitats with native vegetation for stiletto fly species. The local distributions of stiletto fly species at the Guadalupe-Nipomo dune system allow the community to be divided into three assemblages; active dune, pioneer scrub, and scrub-riparian. These assemblages may be applicable to other coastal dune stiletto fly communities, and may have particular relevance to stiletto fly species collected in European coastal dunes. The results from this study provide a descriptive framework for studies testing habitat selection in coastal dune stiletto fly species and inform conservation of threatened dune insects. PMID:17119624
Data Management System for the National Energy-Water System (NEWS) Assessment Framework
NASA Astrophysics Data System (ADS)
Corsi, F.; Prousevitch, A.; Glidden, S.; Piasecki, M.; Celicourt, P.; Miara, A.; Fekete, B. M.; Vorosmarty, C. J.; Macknick, J.; Cohen, S. M.
2015-12-01
Aiming at providing a comprehensive assessment of the water-energy nexus, the National Energy-Water System (NEWS) project requires the integration of data to support a modeling framework that links climate, hydrological, power production, transmission, and economical models. Large amounts of Georeferenced data has to be streamed to the components of the inter-disciplinary model to explore future challenges and tradeoffs in the US power production, based on climate scenarios, power plant locations and technologies, available water resources, ecosystem sustainability, and economic demand. We used open source and in-house build software components to build a system that addresses two major data challenges: On-the-fly re-projection, re-gridding, interpolation, extrapolation, nodata patching, merging, temporal and spatial aggregation, of static and time series datasets in virtually any file formats and file structures, and any geographic extent for the models I/O, directly at run time; Comprehensive data management based on metadata cataloguing and discovery in repositories utilizing the MAGIC Table (Manipulation and Geographic Inquiry Control database). This innovative concept allows models to access data on-the-fly by data ID, irrespective of file path, file structure, file format and regardless its GIS specifications. In addition, a web-based information and computational system is being developed to control the I/O of spatially distributed Earth system, climate, and hydrological, power grid, and economical data flow within the NEWS framework. The system allows scenario building, data exploration, visualization, querying, and manipulation any loaded gridded, point, and vector polygon dataset. The system has demonstrated its potential for applications in other fields of Earth science modeling, education, and outreach. Over time, this implementation of the system will provide near real-time assessment of various current and future scenarios of the water-energy nexus.
Progressively consolidating historical visual explorations for new discoveries
NASA Astrophysics Data System (ADS)
Zhao, Kaiyu; Ward, Matthew O.; Rundensteiner, Elke A.; Higgins, Huong N.
2013-12-01
A significant task within data mining is to identify data models of interest. While facilitating the exploration tasks, most visualization systems do not make use of all the data models that are generated during the exploration. In this paper, we introduce a system that allows the user to gain insights from the data space progressively by forming data models and consolidating the generated models on the fly. Each model can be a a computationally extracted or user-defined subset that contains a certain degree of interest and might lead to some discoveries. When the user generates more and more data models, the degree of interest of some portion of some models will either grow (indicating higher occurrence) or will fluctuate or decrease (corresponding to lower occurrence). Our system maintains a collection of such models and accumulates the interestingness of each model into a consolidated model. In order to consolidate the models, the system summarizes the associations between the models in the collection and identifies support (models reinforce each other), complementary (models complement each other), and overlap of the models. The accumulated interestingness keeps track of historical exploration and helps the user summarize their findings which can lead to new discoveries. This mechanism for integrating results from multiple models can be applied to a wide range of decision support systems. We demonstrate our system in a case study involving the financial status of US companies.
The Impact of Structural Vibration on Flying Qualities of a Supersonic Transport
NASA Technical Reports Server (NTRS)
Raney, David L.; Jackson, E. Bruce; Buttrill, Carey S.; Adams, William M.
2001-01-01
A piloted simulation experiment has been conducted in the NASA Langley Visual/Motion Simulator facility to address the impact of dynamic aeroelastic effects on flying qualities of a supersonic transport. The intent of this experiment was to determine the effectiveness of several measures that may be taken to reduce the impact of aircraft flexibility on piloting tasks. Potential solutions that were examined included structural stiffening, active vibration suppression, and elimination of visual cues associated with the elastic modes. A series of parametric configurations was evaluated by six test pilots for several types of maneuver tasks. During the investigation, several incidents were encountered in which cockpit vibrations due to elastic modes fed back into the control stick through involuntary motions of the pilot's upper body and arm. The phenomenon, referred to as biodynamic coupling, is evidenced by a resonant peak in the power spectrum of the pilot's stick inputs at a structural mode frequency. The results of the investigation indicate that structural stiffening and compensation of the visual display were of little benefit in alleviating the impact of elastic dynamics on the piloting tasks, while increased damping and elimination of control-effector excitation of the lowest frequency modes offered great improvements when applied in sufficient degree.
Visual ecology and potassium conductances of insect photoreceptors.
Frolov, Roman; Immonen, Esa-Ville; Weckström, Matti
2016-04-01
Voltage-activated potassium channels (Kv channels) in the microvillar photoreceptors of arthropods are responsible for repolarization and regulation of photoreceptor signaling bandwidth. On the basis of analyzing Kv channels in dipteran flies, it was suggested that diurnal, rapidly flying insects predominantly express sustained K(+) conductances, whereas crepuscular and nocturnally active animals exhibit strongly inactivating Kv conductances. The latter was suggested to function for minimizing cellular energy consumption. In this study we further explore the evolutionary adaptations of the photoreceptor channelome to visual ecology and behavior by comparing K(+) conductances in 15 phylogenetically diverse insects, using patch-clamp recordings from dissociated ommatidia. We show that rapid diurnal flyers such as the blowfly (Calliphora vicina) and the honeybee (Apis mellifera) express relatively large noninactivating Kv conductances, conforming to the earlier hypothesis in Diptera. Nocturnal and/or slow-moving species do not in general exhibit stronger Kv conductance inactivation in the physiological membrane voltage range, but the photoreceptors in species that are known to rely more on vision behaviorally had higher densities of sustained Kv conductances than photoreceptors of less visually guided species. No statistically significant trends related to visual performance could be identified for the rapidly inactivating Kv conductances. Counterintuitively, strong negative correlations were observed between photoreceptor capacitance and specific membrane conductance for both sustained and inactivating fractions of Kv conductance, suggesting insignificant evolutionary pressure to offset negative effects of high capacitance on membrane filtering with increased conductance. Copyright © 2016 the American Physiological Society.
Inspection of Pole-Like Structures Using a Visual-Inertial Aided VTOL Platform with Shared Autonomy
Sa, Inkyu; Hrabar, Stefan; Corke, Peter
2015-01-01
This paper presents an algorithm and a system for vertical infrastructure inspection using a vertical take-off and landing (VTOL) unmanned aerial vehicle and shared autonomy. Inspecting vertical structures such as light and power distribution poles is a difficult task that is time-consuming, dangerous and expensive. Recently, micro VTOL platforms (i.e., quad-, hexa- and octa-rotors) have been rapidly gaining interest in research, military and even public domains. The unmanned, low-cost and VTOL properties of these platforms make them ideal for situations where inspection would otherwise be time-consuming and/or hazardous to humans. There are, however, challenges involved with developing such an inspection system, for example flying in close proximity to a target while maintaining a fixed stand-off distance from it, being immune to wind gusts and exchanging useful information with the remote user. To overcome these challenges, we require accurate and high-update rate state estimation and high performance controllers to be implemented onboard the vehicle. Ease of control and a live video feed are required for the human operator. We demonstrate a VTOL platform that can operate at close-quarters, whilst maintaining a safe stand-off distance and rejecting environmental disturbances. Two approaches are presented: Position-Based Visual Servoing (PBVS) using an Extended Kalman Filter (EKF) and estimator-free Image-Based Visual Servoing (IBVS). Both use monocular visual, inertia, and sonar data, allowing the approaches to be applied for indoor or GPS-impaired environments. We extensively compare the performances of PBVS and IBVS in terms of accuracy, robustness and computational costs. Results from simulations and indoor/outdoor (day and night) flight experiments demonstrate the system is able to successfully inspect and circumnavigate a vertical pole. PMID:26340631
Ma, Jingqun; Brennan, Kaelan J; D'Aloia, Mitch R; Pascuzzi, Pete E; Weake, Vikki M
2016-08-09
The Spt-Ada-Gcn5 Acetyltransferase (SAGA) complex is a transcriptional coactivator with histone acetylase and deubiquitinase activities that plays an important role in visual development and function. In Drosophila melanogaster, four SAGA subunits are required for the deubiquitination of monoubiquitinated histone H2B (ubH2B): Nonstop, Sgf11, E(y)2, and Ataxin 7. Mutations that disrupt SAGA deubiquitinase activity cause defects in neuronal connectivity in the developing Drosophila visual system. In addition, mutations in SAGA result in the human progressive visual disorder spinocerebellar ataxia type 7 (SCA7). Glial cells play a crucial role in both the neuronal connectivity defect in nonstop and sgf11 flies, and in the retinal degeneration observed in SCA7 patients. Thus, we sought to identify the gene targets of SAGA deubiquitinase activity in glia in the Drosophila larval central nervous system. To do this, we enriched glia from wild-type, nonstop, and sgf11 larval optic lobes using affinity-purification of KASH-GFP tagged nuclei, and then examined each transcriptome using RNA-seq. Our analysis showed that SAGA deubiquitinase activity is required for proper expression of 16% of actively transcribed genes in glia, especially genes involved in proteasome function, protein folding and axon guidance. We further show that the SAGA deubiquitinase-activated gene Multiplexin (Mp) is required in glia for proper photoreceptor axon targeting. Mutations in the human ortholog of Mp, COL18A1, have been identified in a family with a SCA7-like progressive visual disorder, suggesting that defects in the expression of this gene in SCA7 patients could play a role in the retinal degeneration that is unique to this ataxia. Copyright © 2016 Ma et al.
Perceived orientation of a runway model in nonpilots during simulated night approaches to landing.
DOT National Transportation Integrated Search
1977-07-01
Illusions due to reduced visual cues at night have long been cited as contributing to the dangerous tendency of pilots to fly too low during night landing approaches. The cue of motion parallax (a difference in rate of apparent movement of objects in...
Vision: a moving hill for spatial updating on the fly.
Stanford, Terrence R
2015-02-02
A recent study reveals a dynamic neural map that provides a continuous representation of remembered visual stimulus locations with respect to constantly changing gaze. This finding suggests a new mechanistic framework for understanding the spatiotemporal dynamics of goal-directed action. Copyright © 2015 Elsevier Ltd. All rights reserved.
Space shuttle flying qualities and criteria assessment
NASA Technical Reports Server (NTRS)
Myers, T. T.; Johnston, D. E.; Mcruer, Duane T.
1987-01-01
Work accomplished under a series of study tasks for the Flying Qualities and Flight Control Systems Design Criteria Experiment (OFQ) of the Shuttle Orbiter Experiments Program (OEX) is summarized. The tasks involved review of applicability of existing flying quality and flight control system specification and criteria for the Shuttle; identification of potentially crucial flying quality deficiencies; dynamic modeling of the Shuttle Orbiter pilot/vehicle system in the terminal flight phases; devising a nonintrusive experimental program for extraction and identification of vehicle dynamics, pilot control strategy, and approach and landing performance metrics, and preparation of an OEX approach to produce a data archive and optimize use of the data to develop flying qualities for future space shuttle craft in general. Analytic modeling of the Orbiter's unconventional closed-loop dynamics in landing, modeling pilot control strategies, verification of vehicle dynamics and pilot control strategy from flight data, review of various existent or proposed aircraft flying quality parameters and criteria in comparison with the unique dynamic characteristics and control aspects of the Shuttle in landing; and finally a summary of conclusions and recommendations for developing flying quality criteria and design guides for future Shuttle craft.
An insect-inspired model for visual binding II: functional analysis and visual attention.
Northcutt, Brandon D; Higgins, Charles M
2017-04-01
We have developed a neural network model capable of performing visual binding inspired by neuronal circuitry in the optic glomeruli of flies: a brain area that lies just downstream of the optic lobes where early visual processing is performed. This visual binding model is able to detect objects in dynamic image sequences and bind together their respective characteristic visual features-such as color, motion, and orientation-by taking advantage of their common temporal fluctuations. Visual binding is represented in the form of an inhibitory weight matrix which learns over time which features originate from a given visual object. In the present work, we show that information represented implicitly in this weight matrix can be used to explicitly count the number of objects present in the visual image, to enumerate their specific visual characteristics, and even to create an enhanced image in which one particular object is emphasized over others, thus implementing a simple form of visual attention. Further, we present a detailed analysis which reveals the function and theoretical limitations of the visual binding network and in this context describe a novel network learning rule which is optimized for visual binding.
Control system of hexacopter using color histogram footprint and convolutional neural network
NASA Astrophysics Data System (ADS)
Ruliputra, R. N.; Darma, S.
2017-07-01
The development of unmanned aerial vehicles (UAV) has been growing rapidly in recent years. The use of logic thinking which is implemented into the program algorithms is needed to make a smart system. By using visual input from a camera, UAV is able to fly autonomously by detecting a target. However, some weaknesses arose as usage in the outdoor environment might change the target's color intensity. Color histogram footprint overcomes the problem because it divides color intensity into separate bins that make the detection tolerant to the slight change of color intensity. Template matching compare its detection result with a template of the reference image to determine the target position and use it to position the vehicle in the middle of the target with visual feedback control based on Proportional-Integral-Derivative (PID) controller. Color histogram footprint method localizes the target by calculating the back projection of its histogram. It has an average success rate of 77 % from a distance of 1 meter. It can position itself in the middle of the target by using visual feedback control with an average positioning time of 73 seconds. After the hexacopter is in the middle of the target, Convolutional Neural Networks (CNN) classifies a number contained in the target image to determine a task depending on the classified number, either landing, yawing, or return to launch. The recognition result shows an optimum success rate of 99.2 %.
NASA advanced design program. Design and analysis of a radio-controlled flying wing aircraft
NASA Technical Reports Server (NTRS)
1993-01-01
The main challenge of this project was to design an aircraft that will achieve stability while flying without a horizontal tail. The project focused on both the design, analysis and construction of a remotely piloted, elliptical shaped flying wing. The design team was composed of four sub-groups each of which dealt with the different aspects of the design, namely aerodynamics, stability and control, propulsion, and structures. Each member of the team initially researched the background information pertaining to specific facets of the project. Since previous work on this topic was limited, most of the focus of the project was directed towards developing an understanding of the natural instability of the aircraft. Once the design team entered the conceptual stage of the project, a series of compromises had to be made to satisfy the unique requirements of each sub-group. As a result of the numerous calculations and iterations necessary, computers were utilized extensively. In order to visualize the design and layout of the wing, engines and control surfaces, a solid modeling package was used to evaluate optimum design placements. When the design was finalized, construction began with the help of all the members of the project team. The nature of the carbon composite construction process demanded long hours of manual labor. The assembly of the engine systems also required precision hand work. The final product of this project is the Elang, a one-of-a-kind remotely piloted aircraft of composite construction powered by two ducted fan engines.
NASA Technical Reports Server (NTRS)
Bergeron, H. P.; Haynie, A. T.; Mcdede, J. B.
1980-01-01
A general aviation single pilot instrument flight rule simulation capability was developed. Problems experienced by single pilots flying in IFR conditions were investigated. The simulation required a three dimensional spatial navaid environment of a flight navigational area. A computer simulation of all the navigational aids plus 12 selected airports located in the Washington/Norfolk area was developed. All programmed locations in the list were referenced to a Cartesian coordinate system with the origin located at a specified airport's reference point. All navigational aids with their associated frequencies, call letters, locations, and orientations plus runways and true headings are included in the data base. The simulation included a TV displayed out-the-window visual scene of country and suburban terrain and a scaled model runway complex. Any of the programmed runways, with all its associated navaids, can be referenced to a runway on the airport in this visual scene. This allows a simulation of a full mission scenario including breakout and landing.
Intravitreal Phacoemulsification Using Torsional Handpiece for Retained Lens Fragments
Kumar, Vinod; Takkar, Brijesh
2016-01-01
Purpose: To evaluate the results of intravitreal phacoemulsification with torsional hand piece in eyes with posteriorly dislocated lens fragments. Methods: In this prospective, interventional case series, 15 eyes with retained lens fragments following phacoemulsification were included. All patients underwent standard three-port pars plana vitrectomy and intravitreal phacoemulsification using sleeveless, torsional hand piece (OZiL™, Alcon's Infiniti Vision System). Patients were followed up for a minimum of six months to evaluate the visual outcomes and complications. Results: The preoperative best-corrected visual acuity (BCVA) ranged from light perception to 0.3. No complications such as thermal burns of the scleral wound, retinal damage due to flying lens fragments, or difficult lens aspiration occurred during intravitreal phacoemulsification. Mean post-operative BCVA at the final follow-up was 0.5. Two eyes developed cystoid macular edema, which was managed medically. No retinal detachment was noted. Conclusion: Intravitreal phacoemulsification using torsional hand piece is a safe and effective alternative to conventional longitudinal phacofragmentation. PMID:27621783
Lin, Chih-Yung; Chuang, Chao-Chun; Hua, Tzu-En; Chen, Chun-Chao; Dickson, Barry J; Greenspan, Ralph J; Chiang, Ann-Shyn
2013-05-30
How the brain perceives sensory information and generates meaningful behavior depends critically on its underlying circuitry. The protocerebral bridge (PB) is a major part of the insect central complex (CX), a premotor center that may be analogous to the human basal ganglia. Here, by deconstructing hundreds of PB single neurons and reconstructing them into a common three-dimensional framework, we have constructed a comprehensive map of PB circuits with labeled polarity and predicted directions of information flow. Our analysis reveals a highly ordered information processing system that involves directed information flow among CX subunits through 194 distinct PB neuron types. Circuitry properties such as mirroring, convergence, divergence, tiling, reverberation, and parallel signal propagation were observed; their functional and evolutional significance is discussed. This layout of PB neuronal circuitry may provide guidelines for further investigations on transformation of sensory (e.g., visual) input into locomotor commands in fly brains. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.
Evolution of Biological Image Stabilization.
Hardcastle, Ben J; Krapp, Holger G
2016-10-24
The use of vision to coordinate behavior requires an efficient control design that stabilizes the world on the retina or directs the gaze towards salient features in the surroundings. With a level gaze, visual processing tasks are simplified and behaviorally relevant features from the visual environment can be extracted. No matter how simple or sophisticated the eye design, mechanisms have evolved across phyla to stabilize gaze. In this review, we describe functional similarities in eyes and gaze stabilization reflexes, emphasizing their fundamental role in transforming sensory information into motor commands that support postural and locomotor control. We then focus on gaze stabilization design in flying insects and detail some of the underlying principles. Systems analysis reveals that gaze stabilization often involves several sensory modalities, including vision itself, and makes use of feedback as well as feedforward signals. Independent of phylogenetic distance, the physical interaction between an animal and its natural environment - its available senses and how it moves - appears to shape the adaptation of all aspects of gaze stabilization. Copyright © 2016 Elsevier Ltd. All rights reserved.
USDA-ARS?s Scientific Manuscript database
Mass trapping and attract-and-kill bait stations are two attractant based systems that are being used or are under development as pesticide alternatives for control of a number of pest tephritid fruit flies. Results of field trials for suppression of Caribbean fruit flies in guava orchards in Florid...
Using Visual Odometry to Estimate Position and Attitude
NASA Technical Reports Server (NTRS)
Maimone, Mark; Cheng, Yang; Matthies, Larry; Schoppers, Marcel; Olson, Clark
2007-01-01
A computer program in the guidance system of a mobile robot generates estimates of the position and attitude of the robot, using features of the terrain on which the robot is moving, by processing digitized images acquired by a stereoscopic pair of electronic cameras mounted rigidly on the robot. Developed for use in localizing the Mars Exploration Rover (MER) vehicles on Martian terrain, the program can also be used for similar purposes on terrestrial robots moving in sufficiently visually textured environments: examples include low-flying robotic aircraft and wheeled robots moving on rocky terrain or inside buildings. In simplified terms, the program automatically detects visual features and tracks them across stereoscopic pairs of images acquired by the cameras. The 3D locations of the tracked features are then robustly processed into an estimate of overall vehicle motion. Testing has shown that by use of this software, the error in the estimate of the position of the robot can be limited to no more than 2 percent of the distance traveled, provided that the terrain is sufficiently rich in features. This software has proven extremely useful on the MER vehicles during driving on sandy and highly sloped terrains on Mars.
Motion transparency: making models of motion perception transparent.
Snowden; Verstraten
1999-10-01
In daily life our visual system is bombarded with motion information. We see cars driving by, flocks of birds flying in the sky, clouds passing behind trees that are dancing in the wind. Vision science has a good understanding of the first stage of visual motion processing, that is, the mechanism underlying the detection of local motions. Currently, research is focused on the processes that occur beyond the first stage. At this level, local motions have to be integrated to form objects, define the boundaries between them, construct surfaces and so on. An interesting, if complicated case is known as motion transparency: the situation in which two overlapping surfaces move transparently over each other. In that case two motions have to be assigned to the same retinal location. Several researchers have tried to solve this problem from a computational point of view, using physiological and psychophysical results as a guideline. We will discuss two models: one uses the traditional idea known as 'filter selection' and the other a relatively new approach based on Bayesian inference. Predictions from these models are compared with our own visual behaviour and that of the neural substrates that are presumed to underlie these perceptions.
The importance of being top-heavy: Intrinsic stability of flapping flight
NASA Astrophysics Data System (ADS)
Ristroph, Leif; Liu, Bin; Zhang, Jun
2011-11-01
We explore the stability of flapping flight in a model system that consists of a pyramid-shaped object that freely hovers in a vertically oscillating airflow. Such a ``bug'' not only generates sufficient aerodynamic force to keep aloft but also robustly maintains balance during free-flight. Flow visualization reveals that both weight support and intrinsic stability result from the periodic shedding of dipolar vortices. Counter-intuitively, the observed pattern of vortex shedding suggests that stability requires a high center-of-mass, which we verify by comparing the performance of top- and bottom-heavy bugs. Finally, we visit a zoo of other flapping flyers, including Mary Poppins' umbrella, a flying saucer or UFO, and Da Vinci's helicopter.
Effectively Transforming IMC Flight into VMC Flight: An SVS Case Study
NASA Technical Reports Server (NTRS)
Glaab, Louis J.; Hughes, Monic F.; Parrish, Russell V.; Takallu, Mohammad A.
2006-01-01
A flight-test experiment was conducted using the NASA LaRC Cessna 206 aircraft. Four primary flight and navigation display concepts, including baseline and Synthetic Vision System (SVS) concepts, were evaluated in the local area of Roanoke Virginia Airport, flying visual and instrument approach procedures. A total of 19 pilots, from 3 pilot groups reflecting the diverse piloting skills of the GA population, served as evaluation pilots. Multi-variable Discriminant Analysis was applied to three carefully selected and markedly different operating conditions with conventional instrumentation to provide an extension of traditional analysis methods as well as provide an assessment of the effectiveness of SVS displays to effectively transform IMC flight into VMC flight.
NASA Astrophysics Data System (ADS)
Winckler, J. R.; Erickson, K. N.; Abe, Y.; Steffen, J. E.; Malcolm, P. R.
1985-07-01
Orthogonal probes on a free-flying plasma diagnostics payload are used to study ELF electric disturbances in the auroral ionosphere that are due to the injection of powerful electron beams. Frequency spectrograms are presented for various pitch angles, pulsing characteristics, and other properties of the injected beams; the large scale DC ionospheric convection electric field is measured, together with auroral particle precipitation, visual auroral forms, and ionospheric parameters. In view of the experimental results obtained, it is postulated that the observed ELF waves are in the Alfven and drift modes, and are generated by the positive vehicle potential during beam injection.
To develop a flying fish egg inspection system by a digital imaging base system
NASA Astrophysics Data System (ADS)
Chen, Chun-Jen; Jywe, Wenyuh; Hsieh, Tung-Hsien; Chen, Chien Hung
2015-07-01
This paper develops an automatic optical inspection system for flying fish egg quality inspection. The automatic optical inspection system consists of a 2-axes stage, a digital camera, a lens, a LED light source, a vacuum generator, a tube and a tray. This system can automatically find the particle on the flying egg tray and used stage to driver the tube onto the particle. Then use straw and vacuum generator to pick up the particle. The system pick rate is about 30 particles per minute.
Evaluation of XV-15 tilt rotor aircraft for flying qualities research application
NASA Technical Reports Server (NTRS)
Radford, R. C.; Schelhorn, A. E.; Siracuse, R. J.; Till, R. D.; Wasserman, R.
1976-01-01
The results of a design review study and evaluation of the XV-15 Tilt Rotor Research Aircraft for flying qualities research application are presented. The objectives of the program were to determine the capability of the XV-15 aircraft and the V/STOLAND system as a safe, inflight facility to provide meaningful research data on flying qualities, flight control systems, and information display systems.
van der Niet, Timotheüs; Hansen, Dennis M; Johnson, Steven D
2011-05-01
Although pollination of plants that attract flies by resembling their carrion brood and food sites has been reported in several angiosperm families, there has been very little work done on the level of specificity in carrion mimicry systems and the importance of plant cues in mediating such specialization. Specificity may be expected, as carrion-frequenting flies often exploit different niches, which has been interpreted as avoidance of interspecific competition. Interactions between the orchid Satyrium pumilum and a local assemblage of carrion flies were investigated, and the functional significance of floral traits, especially scent, tested. Pollination success and the incidence of pollinator-mediated self-pollination were measured and these were compared with values for orchids with sexual- and food-deceptive pollination systems. Observations of insect visitation to animal carcasses and to flowers showed that the local assemblage of carrion flies was dominated by blow flies (Calliphoridae), house flies (Muscidae) and flesh flies (Sarcophagidae), but flowers of the orchid were pollinated exclusively by flesh flies, with a strong bias towards females that sometimes deposited live larvae on flowers. A trend towards similar partitioning of fly taxa was found in an experiment that tested the effect of large versus small carrion quantities on fly attraction. GC-MS analysis showed that floral scent is dominated by oligosulfides, 2-heptanone, p-cresol and indole, compounds that also dominate carrion scent. Flesh flies did not distinguish between floral and carrion scent in a choice experiment using olfactory cues only, which also showed that scent alone is responsible for fly attraction. Pollination success was relatively high (31·5 % of flowers), but tracking of stained pollinia also revealed that a relatively high percentage (46 %) of pollen deposited on stigmas originates from the same plant. Satyrium pumilum selectively attracts flesh flies, probably because its relatively weak scent resembles that of the small carrion on which these flies predominate. In this way, the plants exploit a specific subset of the insect assemblage associated with carrion. Pollination rates and levels of self-pollination were high compared with those in other deceptive orchids and it is therefore unlikely that this mimicry system evolved to promote outcrossing.
Description and Flight Test Results of the NASA F-8 Digital Fly-by-Wire Control System
NASA Technical Reports Server (NTRS)
1975-01-01
A NASA program to develop digital fly-by-wire (DFBW) technology for aircraft applications is discussed. Phase I of the program demonstrated the feasibility of using a digital fly-by-wire system for aircraft control through developing and flight testing a single channel system, which used Apollo hardware, in an F-8C airplane. The objective of Phase II of the program is to establish a technology base for designing practical DFBW systems. It will involve developing and flight testing a triplex digital fly-by-wire system using state-of-the-art airborne computers, system hardware, software, and redundancy concepts. The papers included in this report describe the Phase I system and its development and present results from the flight program. Man-rated flight software and the effects of lightning on digital flight control systems are also discussed.
Lateral-Directional Eigenvector Flying Qualities Guidelines for High Performance Aircraft
NASA Technical Reports Server (NTRS)
Davidson, John B.; Andrisani, Dominick, II
1996-01-01
This report presents the development of lateral-directional flying qualities guidelines with application to eigenspace (eigenstructure) assignment methods. These guidelines will assist designers in choosing eigenvectors to achieve desired closed-loop flying qualities or performing trade-offs between flying qualities and other important design requirements, such as achieving realizable gain magnitudes or desired system robustness. This has been accomplished by developing relationships between the system's eigenvectors and the roll rate and sideslip transfer functions. Using these relationships, along with constraints imposed by system dynamics, key eigenvector elements are identified and guidelines for choosing values of these elements to yield desirable flying qualities have been developed. Two guidelines are developed - one for low roll-to-sideslip ratio and one for moderate-to-high roll-to-sideslip ratio. These flying qualities guidelines are based upon the Military Standard lateral-directional coupling criteria for high performance aircraft - the roll rate oscillation criteria and the sideslip excursion criteria. Example guidelines are generated for a moderate-to-large, an intermediate, and low value of roll-to-sideslip ratio.
Preliminary Study for a Tetrahedron Formation: Quality Factors and Visualization
NASA Technical Reports Server (NTRS)
Guzman, Jose J.; Schiff, Conrad; Bauer, Frank (Technical Monitor)
2002-01-01
Spacecraft flying in tetrahedron formations are excellent for electromagnetic and plasma studies. The quality of the science recorded is strongly affected by the tetrahedron evolution. This paper is a preliminary study on the computation of quality factors and visualization for a formation of four or five satellites. Four of the satellites are arranged geometrically in a tetrahedron shape. If a fifth satellite is present, it is arbitrarily initialized at the geometric center of the tetrahedron. The fifth satellite could act as a collector or as a spare spacecraft. Tetrahedron natural coordinates are employed for the initialization. The natural orbit evolution is visualized in geocentric equatorial inertial and in geocentric solar magnetospheric coordinates.
Kohlhoff, Kai J.; Jahn, Thomas R.; Lomas, David A.; Dobson, Christopher M.; Crowther, Damian C.; Vendruscolo, Michele
2016-01-01
The use of animal models in medical research provides insights into molecular and cellular mechanisms of human disease, and helps identify and test novel therapeutic strategies. Drosophila melanogaster – the common fruit fly – is one of the most established model organisms, as its study can be performed more readily and with far less expense than for other model animal systems, such as mice, fish, or indeed primates. In the case of fruit flies, standard assays are based on the analysis of longevity and basic locomotor functions. Here we present the iFly tracking system, which enables to increase the amount of quantitative information that can be extracted from these studies, and to reduce significantly the duration and costs associated with them. The iFly system uses a single camera to simultaneously track the trajectories of up to 20 individual flies with about 100μm spatial and 33ms temporal resolution. The statistical analysis of fly movements recorded with such accuracy makes it possible to perform a rapid and fully automated quantitative analysis of locomotor changes in response to a range of different stimuli. We anticipate that the iFly method will reduce very considerably the costs and the duration of the testing of genetic and pharmacological interventions in Drosophila models, including an earlier detection of behavioural changes and a large increase in throughput compared to current longevity and locomotor assays. PMID:21698336
Increasing Reliability with Wireless Instrumentation Systems from Space Shuttle to 'Fly-By-Wireless'
NASA Technical Reports Server (NTRS)
Studor, George
2004-01-01
This slide presentation discusses some of the requirements to allow for "Fly by Wireless". Included in the discussion are: a review of new technologies by decades starting with the 1930's and going through the current decade, structural health monitoring, the requisite system designs, and the vision of flying by wireless.
Visualization and Enabling Science at PO.DAAC
NASA Astrophysics Data System (ADS)
Tauer, E.; To, C.
2017-12-01
Facilitating the identification of appropriate data for scientific inquiry is important for efficient progress, but mechanisms for that identification vary, as does the effectiveness of those mechanisms. Appropriately crafted visualizations provide the means to quickly assess science data and scientific features, but providing the right visualization to the right application can present challenges. Even greater is the challenge of generating and/or re-constituting visualizations on the fly, particularly for large datasets. One avenue to mitigate the challenge is to arrive at an optimized intermediate data format that is tuned for rapid processing without sacrificing the provenance trace back to the original source data. This presentation will discuss the results of trading several current approaches towards an intermediate data format, and suggest a list of key attributes that will facilitate rapid visualization, and in the process, facilitate the identification of the right data for a given application.
Explore the virtual side of earth science
,
1998-01-01
Scientists have always struggled to find an appropriate technology that could represent three-dimensional (3-D) data, facilitate dynamic analysis, and encourage on-the-fly interactivity. In the recent past, scientific visualization has increased the scientist's ability to visualize information, but it has not provided the interactive environment necessary for rapidly changing the model or for viewing the model in ways not predetermined by the visualization specialist. Virtual Reality Modeling Language (VRML 2.0) is a new environment for visualizing 3-D information spaces and is accessible through the Internet with current browser technologies. Researchers from the U.S. Geological Survey (USGS) are using VRML as a scientific visualization tool to help convey complex scientific concepts to various audiences. Kevin W. Laurent, computer scientist, and Maura J. Hogan, technical information specialist, have created a collection of VRML models available through the Internet at Virtual Earth Science (virtual.er.usgs.gov).
Fate of pharmaceuticals and pesticides in fly larvae composting.
Lalander, C; Senecal, J; Gros Calvo, M; Ahrens, L; Josefsson, S; Wiberg, K; Vinnerås, B
2016-09-15
A novel and efficient organic waste management strategy currently gaining great attention is fly larvae composting. High resource recovery efficiency can be achieved in this closed-looped system, but pharmaceuticals and pesticides in waste could potentially accumulate in every loop of the treatment system and spread to the environment. This study evaluated the fate of three pharmaceuticals (carbamazepine, roxithromycin, trimethoprim) and two pesticides (azoxystrobin, propiconazole) in a fly larvae composting system and in a control treatment with no larvae. It was found that the half-life of all five substances was shorter in the fly larvae compost (<10% of control) and no bioaccumulation was detected in the larvae. Fly larvae composting could thus impede the spread of pharmaceuticals and pesticides into the environment. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.
2016-08-24
global sensor field of views (FOVs), mimicking biological systems such as an insect fly eye , but allowing multiple aperture configurations. Due to...synthetic, global sensor field of views (FOVs), mimicking biological systems such as an insect fly eye , but allowing multiple aperture configurations. Due to...such as an insect fly eye , but allowing multiple aperture configurations. Due to the desired nature of distributed networked aerial vehicles (for the
Contact lenses and corrective flying spectacles in military aircrew--implications for flight safety.
Partner, Andrew M; Scott, Robert A H; Shaw, Penny; Coker, William J
2005-07-01
Refractive devices used by aviators need to suit the aerospace environment or their failure can have serious implications. A relatively minor visual disability can result in loss of life and aircraft. We surveyed commonly occurring problems with the different types of refractive correction worn by Royal Air Force (RAF) aircrew over the previous 12 mo. We also asked if they had experienced any flight safety incidents (FSI) relating to their refractive correction. A retrospective anonymous questionnaire survey was given to 700 active aircrew occupationally graded as requiring corrective flying spectacles (CFS) or contact lenses (CL) for flying. 63% (443) of the questionnaires were completed. CL were worn by 53% of aircrew; 71% of them used daily disposable CL. CFS were worn by the remaining 47% of aircrew, 14% of whom used multifocal lenses. Of CFS wearers, 83% reported problems including misting, moving, discomfort, and conflict with helmet-mounted devices (HMD). CL-related ocular symptoms were reported in 67% of wearers including cloudy vision, dry eye, photophobia, red eyes, excessive mucus formation, CL movement, itching, and grittiness. No CL-related FSI were reported over the previous 12 mo compared with 5% CFS-related FSI (p < 0.001). The graded performance of CL for vision, comfort, handling, convenience, and overall satisfaction was significantly higher than for CFS. CFS are associated with problems in terms of comfort and safety. CL are well tolerated by aircrew, and deliver improved visual performance.
Orientation in high-flying migrant insects in relation to flows: mechanisms and strategies
Reynolds, Andy M.; Reynolds, Don R.; Sane, Sanjay P.; Hu, Gao; Chapman, Jason W.
2016-01-01
High-flying insect migrants have been shown to display sophisticated flight orientations that can, for example, maximize distance travelled by exploiting tailwinds, and reduce drift from seasonally optimal directions. Here, we provide a comprehensive overview of the theoretical and empirical evidence for the mechanisms underlying the selection and maintenance of the observed flight headings, and the detection of wind direction and speed, for insects flying hundreds of metres above the ground. Different mechanisms may be used—visual perception of the apparent ground movement or mechanosensory cues maintained by intrinsic features of the wind—depending on circumstances (e.g. day or night migrations). In addition to putative turbulence-induced velocity, acceleration and temperature cues, we present a new mathematical analysis which shows that ‘jerks’ (the time-derivative of accelerations) can provide indicators of wind direction at altitude. The adaptive benefits of the different orientation strategies are briefly discussed, and we place these new findings for insects within a wider context by comparisons with the latest research on other flying and swimming organisms. This article is part of the themed issue ‘Moving in a moving medium: new perspectives on flight’. PMID:27528782
Tinette, Sylvette; Zhang, Lixing; Garnier, Amélie; Engler, Gilbert; Tares, Sophie; Robichon, Alain
2007-01-01
Background Drosophila flies explore the environment very efficiently in order to colonize it. They explore collectively, not individually, so that when a few land on a food spot, they attract the others by signs. This behaviour leads to aggregation of individuals and optimizes the screening of mates and egg-laying on the most favourable food spots. Results Flies perform cycles of exploration/aggregation depending on the resources of the environment. This behavioural ecology constitutes an excellent model for analyzing simultaneous processing of neurosensory information. We reasoned that the decision of flies to land somewhere in order to achieve aggregation is based on simultaneous integration of signals (visual, olfactory, acoustic) during their flight. On the basis of what flies do in nature, we designed laboratory tests to analyze the phenomenon of neuronal coincidence. We screened many mutants of genes involved in neuronal metabolism and the synaptic machinery. Conclusion Mutants of NO-dependent cyclase show a specifically-marked behaviour phenotype, but on the other hand they are associated with moderate biochemical defects. We show that these mutants present errors in integrative and/or coincident processing of signals, which are not reducible to the functions of the peripheral sensory cells. PMID:17683617
Crew and Display Concepts Evaluation for Synthetic / Enhanced Vision Systems
NASA Technical Reports Server (NTRS)
Bailey, Randall E.; Kramer, Lynda J.; Prinzel, Lawrence J., III
2006-01-01
NASA s Synthetic Vision Systems (SVS) project is developing technologies with practical applications that strive to eliminate low-visibility conditions as a causal factor to civil aircraft accidents and replicate the operational benefits of clear day flight operations, regardless of the actual outside visibility condition. Enhanced Vision System (EVS) technologies are analogous and complementary in many respects to SVS, with the principle difference being that EVS is an imaging sensor presentation, as opposed to a database-derived image. The use of EVS in civil aircraft is projected to increase rapidly as the Federal Aviation Administration recently changed the aircraft operating rules under Part 91, revising the flight visibility requirements for conducting operations to civil airports. Operators conducting straight-in instrument approach procedures may now operate below the published approach minimums when using an approved EVS that shows the required visual references on the pilot s Head-Up Display. An experiment was conducted to evaluate the complementary use of SVS and EVS technologies, specifically focusing on new techniques for integration and/or fusion of synthetic and enhanced vision technologies and crew resource management while operating under the newly adopted FAA rules which provide operating credit for EVS. Overall, the experimental data showed that significant improvements in SA without concomitant increases in workload and display clutter could be provided by the integration and/or fusion of synthetic and enhanced vision technologies for the pilot-flying and the pilot-not-flying.
Duerden, Tim
2004-02-01
The first of three papers that considers claims made for the perception or detection of vital energy. Many systems of Complementary and Alternative Medicine (CAM) assume the existence of a vital force that mediates therapeutic efficacy, for example chi or qi in Traditional Chinese medicine. Vital energy directly perceived or imaged that surrounds living organisms is frequently termed the aura. This paper aims to show how phenomena that arise as a consequence of the normal functioning of the human visual system can be inappropriately offered as support of claims for the direct perception of vital energy or the aura. Specifically, contrast and complementary colour phenomena, entoptic phenomena and the deformation phosphene, the 'flying corpuscle effect', the blind spot and the 'reverse telescope effect' are explained and discussed.
Comparative analysis of vestibular ecomorphology in birds.
Benson, Roger B J; Starmer-Jones, Ethan; Close, Roger A; Walsh, Stig A
2017-12-01
The bony labyrinth of vertebrates houses the semicircular canals. These sense rotational accelerations of the head and play an essential role in gaze stabilisation during locomotion. The sizes and shapes of the semicircular canals have hypothesised relationships to agility and locomotory modes in many groups, including birds, and a burgeoning palaeontological literature seeks to make ecological interpretations from the morphology of the labyrinth in extinct species. Rigorous tests of form-function relationships for the vestibular system are required to support these interpretations. We test the hypothesis that the lengths, streamlines and angles between the semicircular canals are related to body size, wing kinematics and flying style in birds. To do this, we applied geometric morphometrics and multivariate phylogenetic comparative methods to a dataset of 64 three-dimensional reconstructions of the endosseous labyrinth obtained using micro-computed tomography scanning of bird crania. A strong relationship between centroid size of the semicircular canals and body size indicates that larger birds have longer semicircular canals compared with their evolutionary relatives. Wing kinematics related to manoeuvrability (and quantified using the brachial index) explain a small additional portion of the variance in labyrinth size. We also find strong evidence for allometric shape change in the semicircular canals of birds, indicating that major aspects of the shape of the avian labyrinth are determined by spatial constraints. The avian braincase accommodates a large brain, a large eye and large semicircular canals compared with other tetrapods. Negative allometry of these structures means that the restriction of space within the braincase is intense in small birds. This may explain our observation that the angles between planes of the semicircular canals of birds deviate more strongly from orthogonality than those of mammals, and especially from agile, gliding and flying mammals. Furthermore, we find little support for relationships between labyrinth shape and flying style or wing kinematics. Overall, our results suggest that the topological problem of fitting long semicircular canals into a spatially constrained braincase is more important in determining the shape of the avian labyrinth than the specifics of locomotory style or agility. Our results tentatively indicate a link between visual acuity and proportional size of the labyrinth among birds. This suggests that the large labyrinths of birds compared with other tetrapods may result from their generally high visual acuities, and not directly from their ability to fly. The endosseous labyrinths of extinct birds and their close dinosaurian relatives may allow broad inferences about flight or vision, but so far provide few specific insights into detailed aspects of locomotion. © 2017 Anatomical Society.
A Systems Approach to Mitigate Oriental Fruit Fly Risk in ‘Sharwil’ Avocados Exported From Hawaii
USDA-ARS?s Scientific Manuscript database
Avocados, Persea americana Miller, grown in Hawaii cannot be exported to the United States mainland without quarantine treatment for melon fly, oriental fruit fly, and Mediterranean fruit fly. The most widely grown cultivar of avocado in Hawaii is ‘Sharwil’. ‘Sharwil’, like other avocado varieties, ...
7 CFR 319.56-55 - Fresh pitaya from certain Central American countries.
Code of Federal Regulations, 2014 CFR
2014-01-01
.... (iii) The NPPO must maintain records of fruit fly detections for each trap, update the records each time the traps are checked, and make the records available to APHIS upon request. The records must be... exporting country must visually inspect a biometric sample of pitaya fruit, jointly approved by APHIS and...
7 CFR 319.56-55 - Fresh pitaya from certain Central American countries.
Code of Federal Regulations, 2013 CFR
2013-01-01
.... (iii) The NPPO must maintain records of fruit fly detections for each trap, update the records each time the traps are checked, and make the records available to APHIS upon request. The records must be... exporting country must visually inspect a biometric sample of pitaya fruit, jointly approved by APHIS and...
Rotary-wing flight test methods used for the evaluation of night vision devices
NASA Astrophysics Data System (ADS)
Haworth, Loran A.; Blanken, Christopher J.; Szoboszlay, Zoltan P.
2001-08-01
The U.S. Army Aviation mission includes flying helicopters at low altitude, at night, and in adverse weather. Night Vision Devices (NVDs) are used to supplement the pilot's visual cues for night flying. As the military requirement to conduct night helicopter operations has increased, the impact of helicopter flight operations with NVD technology in the Degraded Visual Environment (DVE) became increasingly important to quantify. Aeronautical Design Standard-33 (ADS- 33) was introduced to update rotorcraft handling qualities requirements and to quantify the impact of the NVDs in the DVE. As reported in this paper, flight test methodology in ADS-33 has been used by the handling qualities community to measure the impact of NVDs on task performance in the DVE. This paper provides the background and rationale behind the development of ADS-33 flight test methodology for handling qualities in the DVE, as well as the test methodology developed for human factor assessment of NVDs in the DVE. Lessons learned, shortcomings and recommendations for NVD flight test methodology are provided in this paper.
Use of spatial information and search strategies in a water maze analog in Drosophila melanogaster.
Foucaud, Julien; Burns, James G; Mery, Frederic
2010-12-03
Learning the spatial organization of the environment is crucial to fitness in most animal species. Understanding proximate and ultimate factors underpinning spatial memory is thus a major goal in the study of animal behavior. Despite considerable interest in various aspects of its behavior and biology, the model species Drosophila melanogaster lacks a standardized apparatus to investigate spatial learning and memory. We propose here a novel apparatus, the heat maze, conceptually based on the Morris water maze used in rodents. Using the heat maze, we demonstrate that D. melanogaster flies are able to use either proximal or distal visual cues to increase their performance in navigating to a safe zone. We also show that flies are actively using the orientation of distal visual cues when relevant in targeting the safe zone, i.e., Drosophila display spatial learning. Parameter-based classification of search strategies demonstrated the progressive use of spatially precise search strategies during learning. We discuss the opportunity to unravel the mechanistic and evolutionary bases of spatial learning in Drosophila using the heat maze.
Computers Take Flight: A History of NASA's Pioneering Digital Fly-By-Wire Project
NASA Technical Reports Server (NTRS)
Tomayko, James E.
2000-01-01
An overview of the NASA F-8 Fly-by Wire project is presented. The project made two significant contributions to the new technology: (1) a solid design base of techniques that work and those that do not, and (2) credible evidence of good flying qualities and the ability of such a system to tolerate real faults and to continue operation without degradation. In 1972 the F-8C aircraft used in the program became he first digital fly-by-wire aircraft to operate without a mechanical backup system.
The Design and Development of a Management Information System for the Monterey Navy Flying Club.
1986-03-27
Management Information System for the Monterey Navy Flying Club. It supplies the tools necessary to enable the club manager to maintain all club records and generate required administrative and financial reports. The Monterey Navy Flying Club has one of the largest memberships of the Navy sponsored flying clubs. As a result of this large membership and the amount of manual paperwork required to properly maintain club records, the Manager’s ability to provide necessary services and reports in severely hampered. The implementation of an efficient
CO.sub.2 utilization in electrochemical systems
Boxley, Chett; Akash, Akash; Zhao, Qiang
2013-01-22
A process for treating fly ash to render it highly usable as a concrete additive. A quantity of fly ash is obtained that contains carbon and which is considered unusable fly ash for concrete based upon foam index testing. The fly ash is mixed with a quantity of spray dryer ash (SDA) and water to initiate a geopolymerization reaction and form a geopolymerized fly ash. The geopolymerized fly ash is granulated. The geopolymerized fly ash is considered usable fly ash for concrete according to foam index testing. The geopolymerized fly ash may have a foam index less than 40%, and in some cases less than 20%, of the foam index of the untreated fly ash. An optional alkaline activator may be mixed with the fly ash and SDA to facilitate the geopolymerization reaction. The alkaline activator may contain an alkali metal hydroxide, carbonate, silicate, aluminate, or mixtures thereof.
NASA Astrophysics Data System (ADS)
Alin, S. R.; Aalto, R.; Remington, S. M.; Richey, J. E.
2003-12-01
The Fly-Strickland fluvial dispersal system comprises one of the largest river basins in tropical Oceania, ranking among the top 20 rivers in the world for water and sediment discharge. From the New Guinea highlands, these rivers flow >1000 km across lowland tropical floodplains to the Gulf of Papua, with an average annual depth of runoff 100 times that of the Amazon. Within the system, the Strickland has greater sediment discharge and a steeper gradient than the Fly, providing an opportunity to investigate biogeochemical differences associated with particulate flux. For eight lowland sites across the Fly-Strickland river system, we analyzed water and suspended sediment (SS) samples for an initial survey of various carbon cycle parameters. Both the Fly and Strickland Rivers were strongly supersaturated with carbon dioxide (2008-10,479 uatm CO2) and undersaturated with oxygen (1.10-5.48 mg/l O2), with the Fly having higher CO2 and lower O2 concentrations than the Strickland River. These pCO2 and O2 concentrations are comparable to and lower than (respectively) typical values in the Amazon. Measured Fly-Strickland alkalinity values fell in the range of 0.893-1.888 meq, and pH measurements were neutral to slightly alkaline (6.916-7.852). In a sample from a sediment-impoverished tributary from Lake Murray to the Strickland (Herbert R.), pH was neutral (7.060), and alkalinity and pCO2 had their lowest observed values at 0.234 meq and 1407 uatm, respectively. Nutrient concentrations were generally higher in the Strickland ([NO3]=3.36+/-0.69 uM, [PO4]=0.09+/-0.10 uM, and [Si(OH)4]=176.6+/-41.7 uM) than in the Fly River ([NO3]=1.09+/-0.04 uM, [PO4]=0.01+/-0.01 uM, and [Si(OH)4]=110.6+/-4.8 uM). NO3 and PO4 concentrations in the Fly-Strickland river system were lower than in the Amazon, and silicate was comparable. SS concentrations were higher in the Strickland than in the Fly (49.4-231.1 mg/l vs. 19.5-59.6 mg/l). Coarse particulates were organic-poor in the Fly and Strickland rivers, with <1% organic carbon (OC). Fine particulates contained more OC in the Fly (2.32-9.03%) than in the Strickland (0.99-3.08%). In aggregate, the average OC concentration of Fly-Strickland river SS samples is 3.75+/-3.07%, which is substantially higher than %OC values measured in fine particulates in the Amazon. C/N ratios for fine particulate organic matter (FPOM) are higher in the Fly (13.0-24.1) than in the Strickland (9.5-14.4), suggesting a greater contribution of N-poor, terrestrial organics to the Fly River. FPOM δ 13C values in the Fly are more depleted (-31.1--30.3‰ ) than in the Strickland River (-29.6--28.4‰ ). We intend to estimate the total biogeochemical fluxes associated with these reported concentrations. Collectively, these preliminary data on carbon cycling in the Fly and Strickland rivers suggest that this fluvial dispersal system outgases a substantial volume of CO2 each year relative to its area, much like the Amazon River. However, in contrast to the Amazon, where the majority of the sediment from the Andes is trapped in sub-aerial, intra-cratonal basins, most fluviatile sediment is advected beyond the Fly River mouth. Therefore, due to the higher organic content of the fine suspended sediment in this river system, it seems probable that a relatively greater export and burial flux of organic carbon may occur within the delta and shelf deposits of the Gulf of Papua.
Thermal performance analysis of a thermocline thermal energy storage system with FLiNaK molten salt
NASA Astrophysics Data System (ADS)
Liu, C.; Cheng, M. S.; Zhao, B. C.; Dai, Z. M.
2017-01-01
A thermocline thermal storage unit with a heat transfer fluid (HTF) of high-temperature molten salt is considered as one of the most promising methods of thermal storage due to its lower cost and smaller size. The main objective of this work is to analyze the transient behavior of the available molten salt FLiNaK used as the HTF in heat transfer and heat storage in a thermocline thermal energy storage (TES) system. Thermal characteristics including temperature profiles influenced by different inlet velocities of HTF and different void fractions of porous heat storage medium are analyzed. The numerical investigation on the heat storage and heat transfer characteristics of FLiINaK has been carried out. A comparison between two different molten salts, FLiNaK and Hitec, has been explored in this paper with regards to their charging and discharging operations. The results indicate the system with FLiNaK has a greater energy storage capability, a shorter charging time and a higher output power. The numerical investigation reveals heat storage and heat transfer characteristics of the thermocline TES system with FLiNaK, and provide important references for molten salt selection of the TES system in the future.
Richard, Erin Morris; Thiyagarajan, Thirumagal; Bunni, Marlene A.; Basher, Fahmin; Roddy, Patrick O.; Siskind, Leah J.; Nietert, Paul J.; Nowling, Tamara K.
2013-01-01
Systemic Lupus erythematosus (SLE) is an autoimmune disease caused, in part, by abnormalities in cells of the immune system including B and T cells. Genetically reducing globally the expression of the ETS transcription factor FLI1 by 50% in two lupus mouse models significantly improves disease measures and survival through an unknown mechanism. In this study we analyze the effects of reducing FLI1 in the MRL/lpr lupus prone model on T cell function. We demonstrate that adoptive transfer of MRL/lpr Fli1 +/+ or Fli1 +/- T cells and B cells into Rag1-deficient mice results in significantly decreased serum immunoglobulin levels in animals receiving Fli1 +/- lupus T cells compared to animals receiving Fli1 +/+ lupus T cells regardless of the genotype of co-transferred lupus B cells. Ex vivo analyses of MRL/lpr T cells demonstrated that Fli1 +/- T cells produce significantly less IL-4 during early and late disease and exhibited significantly decreased TCR-specific activation during early disease compared to Fli1 +/+ T cells. Moreover, the Fli1 +/- T cells expressed significantly less neuraminidase 1 (Neu1) message and decreased NEU activity during early disease and significantly decreased levels of glycosphingolipids during late disease compared to Fli1 +/+ T cells. FLI1 dose-dependently activated the Neu1 promoter in mouse and human T cell lines. Together, our results suggest reducing FLI1 in lupus decreases the pathogenicity of T cells by decreasing TCR-specific activation and IL-4 production in part through the modulation of glycosphingolipid metabolism. Reducing the expression of FLI1 or targeting the glycosphingolipid metabolic pathway in lupus may serve as a therapeutic approach to treating lupus. PMID:24040398
Richard, Erin Morris; Thiyagarajan, Thirumagal; Bunni, Marlene A; Basher, Fahmin; Roddy, Patrick O; Siskind, Leah J; Nietert, Paul J; Nowling, Tamara K
2013-01-01
Systemic Lupus erythematosus (SLE) is an autoimmune disease caused, in part, by abnormalities in cells of the immune system including B and T cells. Genetically reducing globally the expression of the ETS transcription factor FLI1 by 50% in two lupus mouse models significantly improves disease measures and survival through an unknown mechanism. In this study we analyze the effects of reducing FLI1 in the MRL/lpr lupus prone model on T cell function. We demonstrate that adoptive transfer of MRL/lpr Fli1(+/+) or Fli1(+/-) T cells and B cells into Rag1-deficient mice results in significantly decreased serum immunoglobulin levels in animals receiving Fli1(+/-) lupus T cells compared to animals receiving Fli1(+/+) lupus T cells regardless of the genotype of co-transferred lupus B cells. Ex vivo analyses of MRL/lpr T cells demonstrated that Fli1(+/-) T cells produce significantly less IL-4 during early and late disease and exhibited significantly decreased TCR-specific activation during early disease compared to Fli1(+/+) T cells. Moreover, the Fli1(+/-) T cells expressed significantly less neuraminidase 1 (Neu1) message and decreased NEU activity during early disease and significantly decreased levels of glycosphingolipids during late disease compared to Fli1(+/+) T cells. FLI1 dose-dependently activated the Neu1 promoter in mouse and human T cell lines. Together, our results suggest reducing FLI1 in lupus decreases the pathogenicity of T cells by decreasing TCR-specific activation and IL-4 production in part through the modulation of glycosphingolipid metabolism. Reducing the expression of FLI1 or targeting the glycosphingolipid metabolic pathway in lupus may serve as a therapeutic approach to treating lupus.
Apollo display and keyboard unit (DSKY) used on F-8 DFBW
NASA Technical Reports Server (NTRS)
1996-01-01
The display and keyboard (DSKY) unit used on the F-8 Digital Fly-By-Wire (DFBW) aircraft during Phase I of the fly-by-wire program. Warning lights are in the upper left section, displays in the upper right, and the keyboard is in the lower section. The Apollo flight-control system used in Phase I of the DFBW program had been used previously on the Lunar Module and was incredibly reliable. The DSKY was one element of the system. Also part of the fly-by-wire control system was the inertial platform. Both the computer and the inertial platform required a cooling system that used liquid nitrogen to keep the system within temperature limits. Should the primary flight control system fail, a backup system using three analog computers would automatically take over. The F-8 DFBW had no manual backup. The F-8 Digital Fly-By-Wire (DFBW) flight research project validated the principal concepts of all-electric flight control systems now used on nearly all modern high-performance aircraft and on military and civilian transports. The first flight of the 13-year project was on May 25, 1972, with research pilot Gary E. Krier at the controls of a modified F-8C Crusader that served as the testbed for the fly-by-wire technologies. The project was a joint effort between the NASA Flight Research Center, Edwards, California, (now the Dryden Flight Research Center) and Langley Research Center. It included a total of 211 flights. The last flight was December 16, 1985, with Dryden research pilot Ed Schneider at the controls. The F-8 DFBW system was the forerunner of current fly-by-wire systems used in the space shuttles and on today's military and civil aircraft to make them safer, more maneuverable, and more efficient. Electronic fly-by-wire systems replaced older hydraulic control systems, freeing designers to design aircraft with reduced in-flight stability. Fly-by-wire systems are safer because of their redundancies. They are more maneuverable because computers can command more frequent adjustments than a human pilot can. For airliners, computerized control ensures a smoother ride than a human pilot alone can provide. Digital-fly-by-wire is more efficient because it is lighter and takes up less space than the hydraulic systems it replaced. This either reduces the fuel required to fly or increases the number of passengers or pounds of cargo the aircraft can carry. Digital fly-by-wire is currently used in a variety of aircraft ranging from F/A-18 fighters to the Boeing 777. The DFBW research program is considered one of the most significant and most successful NASA aeronautical programs since the inception of the agency. F-8 aircraft were built originally for the U.S. Navy by LTV Aerospace of Dallas, Texas. The aircraft had a wingspan of 35 feet, 2 inches; was 54 feet, 6 inches long; and was powered by a Pratt & Whitney J57 turbojet engine.
Rodríguez-Gironés, Miguel A.; Trillo, Alejandro; Corcobado, Guadalupe
2013-01-01
The results of behavioural experiments provide important information about the structure and information-processing abilities of the visual system. Nevertheless, if we want to infer from behavioural data how the visual system operates, it is important to know how different learning protocols affect performance and to devise protocols that minimise noise in the response of experimental subjects. The purpose of this work was to investigate how reinforcement schedule and individual variability affect the learning process in a colour discrimination task. Free-flying bumblebees were trained to discriminate between two perceptually similar colours. The target colour was associated with sucrose solution, and the distractor could be associated with water or quinine solution throughout the experiment, or with one substance during the first half of the experiment and the other during the second half. Both acquisition and final performance of the discrimination task (measured as proportion of correct choices) were determined by the choice of reinforcer during the first half of the experiment: regardless of whether bees were trained with water or quinine during the second half of the experiment, bees trained with quinine during the first half learned the task faster and performed better during the whole experiment. Our results confirm that the choice of stimuli used during training affects the rate at which colour discrimination tasks are acquired and show that early contact with a strongly aversive stimulus can be sufficient to maintain high levels of attention during several hours. On the other hand, bees which took more time to decide on which flower to alight were more likely to make correct choices than bees which made fast decisions. This result supports the existence of a trade-off between foraging speed and accuracy, and highlights the importance of measuring choice latencies during behavioural experiments focusing on cognitive abilities. PMID:23951186
Melin, Amanda D; Danosi, Christina F; McCracken, Gary F; Dominy, Nathaniel J
2014-12-01
A nocturnal bottleneck during mammalian evolution left a majority of species with two cone opsins, or dichromatic color vision. Primate trichromatic vision arose from the duplication and divergence of an X-linked opsin gene, and is long attributed to tandem shifts from nocturnality to diurnality and from insectivory to frugivory. Opsin gene variation and at least one duplication event exist in the order Chiroptera, suggesting that trichromatic vision could evolve under favorable ecological conditions. The natural history of the Samoan flying fox (Pteropus samoensis) meets these conditions--it is a large bat that consumes nectar and fruit and demonstrates strong diurnal proclivities. It also possesses a visual system that is strikingly similar to that of primates. To explore the potential for opsin gene duplication and divergence in this species, we sequenced the opsin genes of 11 individuals (19 X-chromosomes) from three South Pacific islands. Our results indicate the uniform presence of two opsins with predicted peak sensitivities of ca. 360 and 553 nm. This result fails to support a causal link between diurnal frugivory and trichromatic vision, although it remains plausible that the diurnal activities of P. samoensis have insufficient antiquity to favor opsin gene renovation.
Fly-by-Wire Systems Enable Safer, More Efficient Flight
NASA Technical Reports Server (NTRS)
2012-01-01
Using the ultra-reliable Apollo Guidance Computer that enabled the Apollo Moon missions, Dryden Flight Research Center engineers, in partnership with industry leaders such as Cambridge, Massachusetts-based Draper Laboratory, demonstrated that digital computers could be used to fly aircraft. Digital fly-by-wire systems have since been incorporated into large airliners, military jets, revolutionary new aircraft, and even cars and submarines.
Flight Control in Complex Environments
2016-10-24
that allow insects, with their miniature brains and limited sensory systems to fly safely through cluttered natural environments . The most significant...specialisations that allow insects, with their miniature brains and limited sensory systems to fly safely through cluttered natural environments . The most...bees have developed more accurate or effective methods for flying safely through gaps than species from less complex environments . Fig. 4: The
NASA Technical Reports Server (NTRS)
Hasler, A. F.; Starr, David (Technical Monitor)
2002-01-01
Spectacular Visualizations of our Blue Marble The NASA/NOAA Electronic Theater presents Earth science observations and visualizations in a historical perspective. Fly in from outer space to the 2002 Winter Olympic Stadium Site of the Olympic Opening and Closing Ceremonies in Salt Lake City. Fly in and through Olympic Alpine Venues using 1 m IKONOS "Spy Satellite" data. Go back to the early weather satellite images from the 1960s and see them contrasted with the latest US and international global satellite weather movies including hurricanes & "tornadoes". See the latest visualizations of spectacular images from NASA/NOAA remote sensing missions like Terra, GOES, TRMM, SeaWiFS, Landsat 7 including new 1 - min GOES rapid scan image sequences of Nov 9th 2001 Midwest tornadic thunderstorms and have them explained. See how High-Definition Television (HDTV) is revolutionizing the way we communicate science. (In cooperation with the American Museum of Natural History in NYC). See dust storms in Africa and smoke plumes from fires in Mexico. See visualizations featured on the covers of Newsweek, TIME, National Geographic, Popular Science & on National & International Network TV. New computer software tools allow us to roam & zoom through massive global images e.g. Landsat tours of the US, and Africa, showing desert and mountain geology as well as seasonal changes in vegetation. See animations of the polar ice packs and the motion of gigantic Antarctic Icebergs from SeaWinds data. Spectacular new visualizations of the global atmosphere & oceans are shown. See vertexes and currents in the global oceans that bring up the nutrients to feed tiny algae and draw the fish, whales and fisherman. See the how the ocean blooms in response to these currents and El Nicola Nina climate changes. See the city lights, fishing fleets, gas flares and biomass burning of the Earth at night observed by the "night-vision" DMSP military satellite.
NASA Astrophysics Data System (ADS)
Zhang, F. H.; Wang, S. F.; An, C. H.; Wang, J.; Xu, Q.
2017-06-01
Large-aperture potassium dihydrogen phosphate (KDP) crystals are widely used in the laser path of inertial confinement fusion (ICF) systems. The most common method of manufacturing half-meter KDP crystals is ultra-precision fly cutting. When processing KDP crystals by ultra-precision fly cutting, the dynamic characteristics of the fly cutting machine and fluctuations in the fly cutting environment are translated into surface errors at different spatial frequency bands. These machining errors should be suppressed effectively to guarantee that KDP crystals meet the full-band machining accuracy specified in the evaluation index. In this study, the anisotropic machinability of KDP crystals and the causes of typical surface errors in ultra-precision fly cutting of the material are investigated. The structures of the fly cutting machine and existing processing parameters are optimized to improve the machined surface quality. The findings are theoretically and practically important in the development of high-energy laser systems in China.
Hansen, Dennis M.; Van der Niet, Timotheüs; Johnson, Steven D.
2012-01-01
Nectar guides, contrasting patterns on flowers that supposedly direct pollinators towards a concealed nectar reward, are taxonomically widespread. However, there have been few studies of their functional significance and effects on plant fitness. Most previous studies focused on pollinator behaviour and used artificial flowers in laboratory settings. We experimentally investigated the role of putative nectar guides in a natural system: the South African iris Lapeirousia oreogena, whose flowers have a clearly visible pattern of six white arrow-markings pointing towards the narrow entrance of the long corolla tube, and its sole pollinator, a long-proboscid nemestrinid fly. We painted over none, some or all of the white arrow-markings with ink that matched the colour of the corolla background. Although arrow-marking removal had little effect on the approaches by flies to flowers from a distance, it dramatically reduced the likelihood of proboscis insertion. Export of pollen dye analogue (an estimate of male fitness) was reduced to almost zero in flowers from which all nectar guides had been removed, and fruit set (a measure of female fitness) was also significantly reduced. Our results confirm that the markings on L. oreogena flowers serve as nectar guides and suggest that they are under strong selective maintenance through both male and female fitness components in this pollination system. PMID:21795269
The Cognitive Challenges of Flying a Remotely Piloted Aircraft
NASA Technical Reports Server (NTRS)
Hobbs, Alan; Cardoza, Colleen; Null, Cynthia
2016-01-01
A large variety of Remotely Piloted Aircraft (RPA) designs are currently in production or in development. These aircraft range from small electric quadcopters that are flown close to the ground within visual range of the operator, to larger systems capable of extended flight in airspace shared with conventional aircraft. Before RPA can operate routinely and safely in civilian airspace, we need to understand the unique human factors associated with these aircraft. The task of flying an RPA in civilian airspace involves challenges common to the operation of other highly-automated systems, but also introduces new considerations for pilot perception, decision-making, and action execution. RPA pilots participated in focus groups where they were asked to recall critical incidents that either presented a threat to safety, or highlighted a case where the pilot contributed to system resilience or mission success. Ninety incidents were gathered from focus-groups. Human factor issues included the impact of reduced sensory cues, traffic separation in the absence of an out-the-window view, control latencies, vigilance during monotonous and ultra-long endurance flights, control station design considerations, transfer of control between control stations, the management of lost link procedures, and decision-making during emergencies. Some of these concerns have received significant attention in the literature, or are analogous to human factors of manned aircraft. The presentation will focus on issues that are poorly understood, and have not yet been the subject of extensive human factors study. Although many of the reported incidents were related to pilot error, the participants also provided examples of the positive contribution that humans make to the operation of highly-automated systems.
Landing flying qualities evaluation criteria for augmented aircraft
NASA Technical Reports Server (NTRS)
Radford, R. C.; Smith, R.; Bailey, R.
1980-01-01
The criteria evaluated were: Calspan Neal-Smith; Onstott (Northrop Time Domain); McDonnell-Douglas Equivalent System Approach; R. H. Smith Criterion. Each criterion was applied to the same set of longitudinal approach and landing flying qualities data. A revised version of the Neal-Smith criterion which is applicable to the landing task was developed and tested against other landing flying qualities data. Results indicated that both the revised Neal-Smith criterion and the Equivalent System Approach are good discriminators of pitch landing flying qualities; Neal-Smith has particular merit as a design guide, while the Equivalent System Approach is well suited for development of appropriate military specification requirements applicable to highly augmented aircraft.
Flight-Test Validation and Flying Qualities Evaluation of a Rotorcraft UAV Flight Control System
NASA Technical Reports Server (NTRS)
Mettler, Bernard; Tuschler, Mark B.; Kanade, Takeo
2000-01-01
This paper presents a process of design and flight-test validation and flying qualities evaluation of a flight control system for a rotorcraft-based unmanned aerial vehicle (RUAV). The keystone of this process is an accurate flight-dynamic model of the aircraft, derived by using system identification modeling. The model captures the most relevant dynamic features of our unmanned rotorcraft, and explicitly accounts for the presence of a stabilizer bar. Using the identified model we were able to determine the performance margins of our original control system and identify limiting factors. The performance limitations were addressed and the attitude control system was 0ptimize.d for different three performance levels: slow, medium, fast. The optimized control laws will be implemented in our RUAV. We will first determine the validity of our control design approach by flight test validating our optimized controllers. Subsequently, we will fly a series of maneuvers with the three optimized controllers to determine the level of flying qualities that can be attained. The outcome enable us to draw important conclusions on the flying qualities requirements for small-scale RUAVs.
Visualization of GPM Standard Products at the Precipitation Processing System (PPS)
NASA Astrophysics Data System (ADS)
Kelley, O.
2010-12-01
Many of the standard data products for the Global Precipitation Measurement (GPM) constellation of satellites will be generated at and distributed by the Precipitation Processing System (PPS) at NASA Goddard. PPS will provide several means to visualize these data products. These visualization tools will be used internally by PPS analysts to investigate potential anomalies in the data files, and these tools will also be made available to researchers. Currently, a free data viewer called THOR, the Tool for High-resolution Observation Review, can be downloaded and installed on Linux, Windows, and Mac OS X systems. THOR can display swath and grid products, and to a limited degree, the low-level data packets that the satellite itself transmits to the ground system. Observations collected since the 1997 launch of the Tropical Rainfall Measuring Mission (TRMM) satellite can be downloaded from the PPS FTP archive, and in the future, many of the GPM standard products will also be available from this FTP site. To provide easy access to this 80 terabyte and growing archive, PPS currently operates an on-line ordering tool called STORM that provides geographic and time searches, browse-image display, and the ability to order user-specified subsets of standard data files. Prior to the anticipated 2013 launch of the GPM core satellite, PPS will expand its visualization tools by integrating an on-line version of THOR within STORM to provide on-the-fly image creation of any portion of an archived data file at a user-specified degree of magnification. PPS will also provide OpenDAP access to the data archive and OGC WMS image creation of both swath and gridded data products. During the GPM era, PPS will continue to provide realtime globally-gridded 3-hour rainfall estimates to the public in a compact binary format (3B42RT) and in a GIS format (2-byte TIFF images + ESRI WorldFiles).
Integrating and Visualizing Tropical Cyclone Data Using the Real Time Mission Monitor
NASA Technical Reports Server (NTRS)
Goodman, H. Michael; Blakeslee, Richard; Conover, Helen; Hall, John; He, Yubin; Regner, Kathryn
2009-01-01
The Real Time Mission Monitor (RTMM) is a visualization and information system that fuses multiple Earth science data sources, to enable real time decision-making for airborne and ground validation experiments. Developed at the NASA Marshall Space Flight Center, RTMM is a situational awareness, decision-support system that integrates satellite imagery, radar, surface and airborne instrument data sets, model output parameters, lightning location observations, aircraft navigation data, soundings, and other applicable Earth science data sets. The integration and delivery of this information is made possible using data acquisition systems, network communication links, network server resources, and visualizations through the Google Earth virtual globe application. RTMM is extremely valuable for optimizing individual Earth science airborne field experiments. Flight planners, scientists, and managers appreciate the contributions that RTMM makes to their flight projects. A broad spectrum of interdisciplinary scientists used RTMM during field campaigns including the hurricane-focused 2006 NASA African Monsoon Multidisciplinary Analyses (NAMMA), 2007 NOAA-NASA Aerosonde Hurricane Noel flight, 2007 Tropical Composition, Cloud, and Climate Coupling (TC4), plus a soil moisture (SMAP-VEX) and two arctic research experiments (ARCTAS) in 2008. Improving and evolving RTMM is a continuous process. RTMM recently integrated the Waypoint Planning Tool, a Java-based application that enables aircraft mission scientists to easily develop a pre-mission flight plan through an interactive point-and-click interface. Individual flight legs are automatically calculated "on the fly". The resultant flight plan is then immediately posted to the Google Earth-based RTMM for interested scientists to view the planned flight track and subsequently compare it to the actual real time flight progress. We are planning additional capabilities to RTMM including collaborations with the Jet Propulsion Laboratory in the joint development of a Tropical Cyclone Integrated Data Exchange and Analysis System (TC IDEAS) which will serve as a web portal for access to tropical cyclone data, visualizations and model output.
Bombykol receptors in the silkworm moth and the fruit fly
Syed, Zainulabeuddin; Kopp, Artyom; Kimbrell, Deborah A.; Leal, Walter S.
2010-01-01
Male moths are endowed with odorant receptors (ORs) to detect species-specific sex pheromones with remarkable sensitivity and selectivity. We serendipitously discovered that an endogenous OR in the fruit fly, Drosophila melanogaster, is highly sensitive to the sex pheromone of the silkworm moth, bombykol. Intriguingly, the fruit fly detectors are more sensitive than the receptors of the silkworm moth, although its ecological significance is unknown. By expression in the “empty neuron” system, we identified the fruit fly bombykol-sensitive OR as DmelOR7a (= DmOR7a). The profiles of this receptor in response to bombykol in the native sensilla (ab4) or expressed in the empty neuron system (ab3 sensilla) are indistinguishable. Both WT and transgenic flies responded with high sensitivity, in a dose-dependent manner, and with rapid signal termination. In contrast, the same empty neuron expressing the moth bombykol receptor, BmorOR1, demonstrated low sensitivity and slow signal inactivation. When expressed in the trichoid sensilla T1 of the fruit fly, the neuron housing BmorOR1 responded with sensitivity comparable to that of the native trichoid sensilla in the silkworm moth. By challenging the native bombykol receptor in the fruit fly with high doses of another odorant to which the receptor responds with the highest sensitivity, we demonstrate that slow signal termination is induced by overdose of a stimulus. As opposed to the empty neuron system in the basiconic sensilla, the structural, biochemical, and/or biophysical features of the sensilla make the T1 trichoid system of the fly a better surrogate for the moth receptor. PMID:20439725
48 CFR 47.403 - Guidelines for implementation of the Fly America Act.
Code of Federal Regulations, 2014 CFR
2014-10-01
... implementation of the Fly America Act. 47.403 Section 47.403 Federal Acquisition Regulations System FEDERAL... Guidelines for implementation of the Fly America Act. This section 47.403 is based on the Guidelines for Implementation of the Fly America Act (case number B-138942), issued by the Comptroller General of the United...
48 CFR 47.403 - Guidelines for implementation of the Fly America Act.
Code of Federal Regulations, 2011 CFR
2011-10-01
... implementation of the Fly America Act. 47.403 Section 47.403 Federal Acquisition Regulations System FEDERAL... Guidelines for implementation of the Fly America Act. This section 47.403 is based on the Guidelines for Implementation of the Fly America Act (case number B-138942), issued by the Comptroller General of the United...
48 CFR 47.403 - Guidelines for implementation of the Fly America Act.
Code of Federal Regulations, 2012 CFR
2012-10-01
... implementation of the Fly America Act. 47.403 Section 47.403 Federal Acquisition Regulations System FEDERAL... Guidelines for implementation of the Fly America Act. This section 47.403 is based on the Guidelines for Implementation of the Fly America Act (case number B-138942), issued by the Comptroller General of the United...
48 CFR 47.403 - Guidelines for implementation of the Fly America Act.
Code of Federal Regulations, 2013 CFR
2013-10-01
... implementation of the Fly America Act. 47.403 Section 47.403 Federal Acquisition Regulations System FEDERAL... Guidelines for implementation of the Fly America Act. This section 47.403 is based on the Guidelines for Implementation of the Fly America Act (case number B-138942), issued by the Comptroller General of the United...
An unsupervised method for quantifying the behavior of paired animals
NASA Astrophysics Data System (ADS)
Klibaite, Ugne; Berman, Gordon J.; Cande, Jessica; Stern, David L.; Shaevitz, Joshua W.
2017-02-01
Behaviors involving the interaction of multiple individuals are complex and frequently crucial for an animal’s survival. These interactions, ranging across sensory modalities, length scales, and time scales, are often subtle and difficult to characterize. Contextual effects on the frequency of behaviors become even more difficult to quantify when physical interaction between animals interferes with conventional data analysis, e.g. due to visual occlusion. We introduce a method for quantifying behavior in fruit fly interaction that combines high-throughput video acquisition and tracking of individuals with recent unsupervised methods for capturing an animal’s entire behavioral repertoire. We find behavioral differences between solitary flies and those paired with an individual of the opposite sex, identifying specific behaviors that are affected by social and spatial context. Our pipeline allows for a comprehensive description of the interaction between two individuals using unsupervised machine learning methods, and will be used to answer questions about the depth of complexity and variance in fruit fly courtship.
Neural mechanisms underlying sensitivity to reverse-phi motion in the fly
Meier, Matthias; Serbe, Etienne; Eichner, Hubert; Borst, Alexander
2017-01-01
Optical illusions provide powerful tools for mapping the algorithms and circuits that underlie visual processing, revealing structure through atypical function. Of particular note in the study of motion detection has been the reverse-phi illusion. When contrast reversals accompany discrete movement, detected direction tends to invert. This occurs across a wide range of organisms, spanning humans and invertebrates. Here, we map an algorithmic account of the phenomenon onto neural circuitry in the fruit fly Drosophila melanogaster. Through targeted silencing experiments in tethered walking flies as well as electrophysiology and calcium imaging, we demonstrate that ON- or OFF-selective local motion detector cells T4 and T5 are sensitive to certain interactions between ON and OFF. A biologically plausible detector model accounts for subtle features of this particular form of illusory motion reversal, like the re-inversion of turning responses occurring at extreme stimulus velocities. In light of comparable circuit architecture in the mammalian retina, we suggest that similar mechanisms may apply even to human psychophysics. PMID:29261684
Neural mechanisms underlying sensitivity to reverse-phi motion in the fly.
Leonhardt, Aljoscha; Meier, Matthias; Serbe, Etienne; Eichner, Hubert; Borst, Alexander
2017-01-01
Optical illusions provide powerful tools for mapping the algorithms and circuits that underlie visual processing, revealing structure through atypical function. Of particular note in the study of motion detection has been the reverse-phi illusion. When contrast reversals accompany discrete movement, detected direction tends to invert. This occurs across a wide range of organisms, spanning humans and invertebrates. Here, we map an algorithmic account of the phenomenon onto neural circuitry in the fruit fly Drosophila melanogaster. Through targeted silencing experiments in tethered walking flies as well as electrophysiology and calcium imaging, we demonstrate that ON- or OFF-selective local motion detector cells T4 and T5 are sensitive to certain interactions between ON and OFF. A biologically plausible detector model accounts for subtle features of this particular form of illusory motion reversal, like the re-inversion of turning responses occurring at extreme stimulus velocities. In light of comparable circuit architecture in the mammalian retina, we suggest that similar mechanisms may apply even to human psychophysics.
Flying qualities - A costly lapse in flight-control design
NASA Technical Reports Server (NTRS)
Berry, D. T.
1982-01-01
Generic problems in advanced aircraft with advanced control systems which suffer from control sensitivity, sluggish response, and pilot-induced oscillation tendencies are examined, with a view to improving techniques for eliminating the problems in the design phase. Results of two NASA and NASA/AIAA workshops reached a consensus that flying qualities criteria do not match control system development, control system designers are not relying on past experience in their field, ground-based simulation is relied on too heavily, and communications between flying qualities and control systems engineers need improvement. A summation is offered in that hardware and software have outstripped the pilot's capacity to use the capabilities which new aircraft offer. The flying qualities data base is stressed to be dynamic, and continually redefining the man/machine relationships.
Calcagno, B; Eyles, D; van Alphen, B; van Swinderen, B
2013-01-08
It has been observed that certain developmental environmental risk factors for schizophrenia when modeled in rodents alter the trajectory of dopaminergic development, leading to persistent behavioural changes in adults. This has recently been articulated as the "dopamine ontogeny hypothesis of schizophrenia". To test one aspect of this hypothesis, namely that transient dopaminergic effects during development modulate attention-like behavior and arousal in adults, we turned to a small-brain model, Drosophila melanogaster. By applying genetic tools allowing transient activation or silencing of dopaminergic neurons in the fly brain, we investigated whether a critical window exists during development when altered dopamine (DA) activity levels could lead to impairments in arousal states in adult animals. We found that increased activity in dopaminergic neurons in later stages of development significantly increased visual responsiveness and locomotion, especially in adult males. This misallocation of visual salience and hyperactivity mimicked the effect of acute methamphetamine feeding to adult flies, suggesting up-regulated DA signaling could result from developmental manipulations. Finally, brain recordings revealed significantly reduced gamma-band activity in adult animals exposed to the transient developmental insult. Together, these data support the idea that transient alterations in DA signaling during development can permanently alter behavior in adults, and that a reductionist model such as Drosophila can be used to investigate potential mechanisms underlying complex cognitive disorders such as schizophrenia.
van der Niet, Timotheüs; Hansen, Dennis M.; Johnson, Steven D.
2011-01-01
Background and Aims Although pollination of plants that attract flies by resembling their carrion brood and food sites has been reported in several angiosperm families, there has been very little work done on the level of specificity in carrion mimicry systems and the importance of plant cues in mediating such specialization. Specificity may be expected, as carrion-frequenting flies often exploit different niches, which has been interpreted as avoidance of interspecific competition. Interactions between the orchid Satyrium pumilum and a local assemblage of carrion flies were investigated, and the functional significance of floral traits, especially scent, tested. Pollination success and the incidence of pollinator-mediated self-pollination were measured and these were compared with values for orchids with sexual- and food-deceptive pollination systems. Methods and Key Results Observations of insect visitation to animal carcasses and to flowers showed that the local assemblage of carrion flies was dominated by blow flies (Calliphoridae), house flies (Muscidae) and flesh flies (Sarcophagidae), but flowers of the orchid were pollinated exclusively by flesh flies, with a strong bias towards females that sometimes deposited live larvae on flowers. A trend towards similar partitioning of fly taxa was found in an experiment that tested the effect of large versus small carrion quantities on fly attraction. GC-MS analysis showed that floral scent is dominated by oligosulfides, 2-heptanone, p-cresol and indole, compounds that also dominate carrion scent. Flesh flies did not distinguish between floral and carrion scent in a choice experiment using olfactory cues only, which also showed that scent alone is responsible for fly attraction. Pollination success was relatively high (31·5 % of flowers), but tracking of stained pollinia also revealed that a relatively high percentage (46 %) of pollen deposited on stigmas originates from the same plant. Conclusions Satyrium pumilum selectively attracts flesh flies, probably because its relatively weak scent resembles that of the small carrion on which these flies predominate. In this way, the plants exploit a specific subset of the insect assemblage associated with carrion. Pollination rates and levels of self-pollination were high compared with those in other deceptive orchids and it is therefore unlikely that this mimicry system evolved to promote outcrossing. PMID:21402538
Results of NASA's First Autonomous Formation Flying Experiment: Earth Observing-1 (EO-1)
NASA Technical Reports Server (NTRS)
Folta, David C.; Hawkins, Albin; Bauer, Frank H. (Technical Monitor)
2001-01-01
NASA's first autonomous formation flying mission completed its primary goal of demonstrating an advanced technology called enhanced formation flying. To enable this technology, the Guidance, Navigation, and Control center at the Goddard Space Flight Center (GSFC) implemented a universal 3-axis formation flying algorithm in an autonomous executive flight code onboard the New Millennium Program's (NMP) Earth Observing-1 (EO-1) spacecraft. This paper describes the mathematical background of the autonomous formation flying algorithm and the onboard flight design and presents the validation results of this unique system. Results from functionality assessment through fully autonomous maneuver control are presented as comparisons between the onboard EO-1 operational autonomous control system called AutoCon(tm), its ground-based predecessor, and a standalone algorithm.
NASA Astrophysics Data System (ADS)
Hakkarinen, C.; Brown, D.; Callahan, J.; hankin, S.; de Koningh, M.; Middleton-Link, D.; Wigley, T.
2001-05-01
A Web-based access system to climate model output data sets for intercomparison and analysis has been produced, using the NOAA-PMEL developed Live Access Server software as host server and Ferret as the data serving and visualization engine. Called ARCAS ("ACACIA Regional Climate-data Access System"), and publicly accessible at http://dataserver.ucar.edu/arcas, the site currently serves climate model outputs from runs of the NCAR Climate System Model for the 21st century, for Business as Usual and Stabilization of Greenhouse Gas Emission scenarios. Users can select, download, and graphically display single variables or comparisons of two variables from either or both of the CSM model runs, averaged for monthly, seasonal, or annual time resolutions. The time length of the averaging period, and the geographical domain for download and display, are fully selectable by the user. A variety of arithmetic operations on the data variables can be computed "on-the-fly", as defined by the user. Expansions of the user-selectable options for defining analysis options, and for accessing other DOD-compatible ("Distributed Ocean Data System-compatible") data sets, residing at locations other than the NCAR hardware server on which ARCAS operates, are planned for this year. These expansions are designed to allow users quick and easy-to-operate web-based access to the largest possible selection of climate model output data sets available throughout the world.
An Automated Flying-Insect Detection System
NASA Technical Reports Server (NTRS)
Vann, Timi; Andrews, Jane C.; Howell, Dane; Ryan, Robert
2007-01-01
An automated flying-insect detection system (AFIDS) was developed as a proof-of-concept instrument for real-time detection and identification of flying insects. This type of system has use in public health and homeland-security decision support, agriculture and military pest management, and/or entomological research. Insects are first lured into the AFIDS integrated sphere by insect attractants. Once inside the sphere, the insect s wing beats cause alterations in light intensity that is detected by a photoelectric sensor. Following detection, the insects are encouraged (with the use of a small fan) to move out of the sphere and into a designated insect trap where they are held for taxonomic identification or serological testing. The acquired electronic wing-beat signatures are preprocessed (Fourier transformed) in real time to display a periodic signal. These signals are sent to the end user where they are graphically. All AFIDS data are preprocessed in the field with the use of a laptop computer equipped with LabVIEW. The AFIDS software can be programmed to run continuously or at specific time intervals when insects are prevalent. A special DC-restored transimpedance amplifier reduces the contributions of low-frequency background light signals, and affords approximately two orders of magnitude greater AC gain than conventional amplifiers. This greatly increases the signal-to-noise ratio and enables the detection of small changes in light intensity. The AFIDS light source consists of high-intensity Al-GaInP light-emitting diodes (LEDs). The AFIDS circuitry minimizes brightness fluctuations in the LEDs and when integrated with an integrating sphere, creates a diffuse uniform light field. The insect wing beats isotropically scatter the diffuse light in the sphere and create wing-beat signatures that are detected by the sensor. This configuration minimizes variations in signal associated with insect flight orientation. Preliminary data indicate that AFIDS has sufficient sensitivity and frequency measuring capability to differentiate between male and female mosquitoes (Figure 1, bottom panel) and fruit flies (data not shown). Similar studies show that AFIDS can be utilized to detect discrete differences between two mosquito species, Aedes aegypti and Aedes albopictus. When fully deployable, a wireless network of AFIDS monitors could be used in combination with other remotely sensed data and visually displayed in a geographic information system (GIS) to provide real-time surveillance (see Figure 2). More accurate and sensitive insect population forecasts and effective rapid response and mitigation of insect issues would then be possible.
A biomimetic vision-based hovercraft accounts for bees' complex behaviour in various corridors.
Roubieu, Frédéric L; Serres, Julien R; Colonnier, Fabien; Franceschini, Nicolas; Viollet, Stéphane; Ruffier, Franck
2014-09-01
Here we present the first systematic comparison between the visual guidance behaviour of a biomimetic robot and those of honeybees flying in similar environments. We built a miniature hovercraft which can travel safely along corridors with various configurations. For the first time, we implemented on a real physical robot the 'lateral optic flow regulation autopilot', which we previously studied computer simulations. This autopilot inspired by the results of experiments on various species of hymenoptera consists of two intertwined feedback loops, the speed and lateral control loops, each of which has its own optic flow (OF) set-point. A heading-lock system makes the robot move straight ahead as fast as 69 cm s(-1) with a clearance from one wall as small as 31 cm, giving an unusually high translational OF value (125° s(-1)). Our biomimetic robot was found to navigate safely along straight, tapered and bent corridors, and to react appropriately to perturbations such as the lack of texture on one wall, the presence of a tapering or non-stationary section of the corridor and even a sloping terrain equivalent to a wind disturbance. The front end of the visual system consists of only two local motion sensors (LMS), one on each side. This minimalistic visual system measuring the lateral OF suffices to control both the robot's forward speed and its clearance from the walls without ever measuring any speeds or distances. We added two additional LMSs oriented at +/-45° to improve the robot's performances in stiffly tapered corridors. The simple control system accounts for worker bees' ability to navigate safely in six challenging environments: straight corridors, single walls, tapered corridors, straight corridors with part of one wall moving or missing, as well as in the presence of wind.
Early warning of active fire hotspots through NASA FIRMS fire information system
NASA Astrophysics Data System (ADS)
Ilavajhala, S.; Davies, D.; Schmaltz, J. E.; Murphy, K. J.
2014-12-01
Forest fires and wildfires can threaten ecosystems, wildlife, property, and often, large swaths of populations. Early warning of active fire hotspots plays a crucial role in planning, managing, and mitigating the damaging effects of wildfires. The NASA Fire Information for Resource Management System (FIRMS) has been providing active fire location information to users in easy-to-use formats for the better part of last decade, with a view to improving the alerting mechanisms and response times to fight forest and wildfires. FIRMS utilizes fires flagged as hotspots by the MODIS instrument flying aboard the Aqua and Terra satellites and sends early warning of detected hotspots via email in near real-time or as daily and weekly summaries. The email alerts can also be customized to send alerts for a particular region of interest, a country, or a specific protected area or park. In addition, a web mapping component, named "Web Fire Mapper" helps query and visualize hotspots. A newer version of Web Fire Mapper is being developed to enhance the existing visualization and alerting capabilities. Plans include supporting near real-time imagery from Aqua and Terra satellites to provide a more helpful context while viewing fires. Plans are also underway to upgrade the email alerts system to provide mobile-formatted messages and short text messages (SMS). The newer version of FIRMS will also allow users to obtain geo-located image snapshots, which can be imported into local GIS software by stakeholders to help further analyses. This talk will discuss the FIRMS system, its enhancements and its role in helping map, alert, and monitor fire hotspots by providing quick data visualization, querying, and download capabilities.
Advanced control technology and airworthiness flying qualities requirements
NASA Technical Reports Server (NTRS)
Snyder, C. T.
1976-01-01
Flying quality requirements are specified in terms of the complete pilot-airframe-systems loop, the task, and the environment. Results from a study of flying qualities are reported. A review of the treatment of failure cases in various flying quality requirements is presented along with a description of the methods used and relevant lessons learned from recent Autoland certification programs.
USDA-ARS?s Scientific Manuscript database
Field trials were conducted to evaluate if filth fly trap efficacy was increased by application of an insecticide to a trap’s exterior. Four Fly Terminator® Pro traps baited with Fly Terminator® attractant were suspended on PVC pipe framing at a Florida waste transfer site. Exterior surfaces of tw...
Wang, Gaoling; Shi, Bingtian; Li, Tao; Zuo, Teng; Wang, Bin; Si, Wei; Xin, Jiuqing; Yang, Kongbin; Shi, Xuanlin; Liu, Siguo; Liu, Henggui
2016-02-29
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major cause of food-borne illness around the world and can have significant health implications in humans, poultry and other animals. Flagellin (FliC) is the primary component of bacterial flagella. It has been shown that the FliC of S. Enteritidis is a significant antigenic structure and can elicit strong humoral responses against S. Enteritidis infection in chickens. Here, we constructed a FliC antigen library using a yeast surface expression system. Yeast cells expressing FliC peptide antigens were labeled with chicken sera against S. Enteritidis and sorted using FACS. The analyses of FliC peptides revealed that the FliC linear antigenicity in chickens resided on three domains which were able to elicit strong humoral responses in vivo. Animal experiments further revealed that the antibodies elicited by these antigenic domains were able to significantly inhibit the invasion of S. Enteritidis into the liver and spleen of chickens. These findings will facilitate our better understanding of the humoral responses elicited by FliC in chickens upon infection by S. Enteritidis. Copyright © 2016 Elsevier B.V. All rights reserved.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Dong, Han; Sharma, Diksha; Badano, Aldo, E-mail: aldo.badano@fda.hhs.gov
2014-12-15
Purpose: Monte Carlo simulations play a vital role in the understanding of the fundamental limitations, design, and optimization of existing and emerging medical imaging systems. Efforts in this area have resulted in the development of a wide variety of open-source software packages. One such package, hybridMANTIS, uses a novel hybrid concept to model indirect scintillator detectors by balancing the computational load using dual CPU and graphics processing unit (GPU) processors, obtaining computational efficiency with reasonable accuracy. In this work, the authors describe two open-source visualization interfaces, webMANTIS and visualMANTIS to facilitate the setup of computational experiments via hybridMANTIS. Methods: Themore » visualization tools visualMANTIS and webMANTIS enable the user to control simulation properties through a user interface. In the case of webMANTIS, control via a web browser allows access through mobile devices such as smartphones or tablets. webMANTIS acts as a server back-end and communicates with an NVIDIA GPU computing cluster that can support multiuser environments where users can execute different experiments in parallel. Results: The output consists of point response and pulse-height spectrum, and optical transport statistics generated by hybridMANTIS. The users can download the output images and statistics through a zip file for future reference. In addition, webMANTIS provides a visualization window that displays a few selected optical photon path as they get transported through the detector columns and allows the user to trace the history of the optical photons. Conclusions: The visualization tools visualMANTIS and webMANTIS provide features such as on the fly generation of pulse-height spectra and response functions for microcolumnar x-ray imagers while allowing users to save simulation parameters and results from prior experiments. The graphical interfaces simplify the simulation setup and allow the user to go directly from specifying input parameters to receiving visual feedback for the model predictions.« less
Newman, David G
2002-11-01
This report describes a case of central serous retinopathy (CSR) in the right eye of a commercial air transport pilot which resulted in a permanent reduction in visual acuity and the loss of his license. The previously fit and well pilot developed sudden loss of central vision, which resolved spontaneously. He then went on to experience recurrent episodes of fluctuating visual acuity (down to 6/60) and visual dysfunction in the right eye. His left eye remained unaffected. Eventually his condition stabilized, and he was left with a permanent reduction in right visual acuity (6/36) with intact peripheral visual fields and a completely normal left eye. After a period of grounding of 12 mo, he sought to have his license reinstated. He was considered to be a functionally monocular pilot, and as such was granted a conditional Class 1 medical category. The aeromedical disposition of this pilot and the issues involved in determining the fitness to fly of pilots with permanent visual defects arising from CSR are discussed.
Visual Associative Learning in Restrained Honey Bees with Intact Antennae
Dobrin, Scott E.; Fahrbach, Susan E.
2012-01-01
A restrained honey bee can be trained to extend its proboscis in response to the pairing of an odor with a sucrose reward, a form of olfactory associative learning referred to as the proboscis extension response (PER). Although the ability of flying honey bees to respond to visual cues is well-established, associative visual learning in restrained honey bees has been challenging to demonstrate. Those few groups that have documented vision-based PER have reported that removing the antennae prior to training is a prerequisite for learning. Here we report, for a simple visual learning task, the first successful performance by restrained honey bees with intact antennae. Honey bee foragers were trained on a differential visual association task by pairing the presentation of a blue light with a sucrose reward and leaving the presentation of a green light unrewarded. A negative correlation was found between age of foragers and their performance in the visual PER task. Using the adaptations to the traditional PER task outlined here, future studies can exploit pharmacological and physiological techniques to explore the neural circuit basis of visual learning in the honey bee. PMID:22701575
Rhodes-Mordov, Elisheva; Katz, Ben; Oberegelsbacher, Claudia; Yasin, Bushra; Tzadok, Hanan; Huber, Armin
2017-01-01
Drosophila photoreceptors respond to oscillating light of high frequency (∼100 Hz), while the detected maximal frequency is modulated by the light rearing conditions, thus enabling high sensitivity to light and high temporal resolution. However, the molecular basis for this adaptive process is unclear. Here, we report that dephosphorylation of the light-activated transient receptor potential (TRP) ion channel at S936 is a fast, graded, light-dependent, and Ca2+-dependent process that is partially modulated by the rhodopsin phosphatase retinal degeneration C (RDGC). Electroretinogram measurements of the frequency response to oscillating lights in vivo revealed that dark-reared flies expressing wild-type TRP exhibited a detection limit of oscillating light at relatively low frequencies, which was shifted to higher frequencies upon light adaptation. Strikingly, preventing phosphorylation of the S936-TRP site by alanine substitution in transgenic Drosophila (trpS936A) abolished the difference in frequency response between dark-adapted and light-adapted flies, resulting in high-frequency response also in dark-adapted flies. In contrast, inserting a phosphomimetic mutation by substituting the S936-TRP site to aspartic acid (trpS936D) set the frequency response of light-adapted flies to low frequencies typical of dark-adapted flies. Light-adapted rdgC mutant flies showed relatively high S936-TRP phosphorylation levels and light–dark phosphorylation dynamics. These findings suggest that RDGC is one but not the only phosphatase involved in pS936-TRP dephosphorylation. Together, this study indicates that TRP channel dephosphorylation is a regulatory process that affects the detection limit of oscillating light according to the light rearing condition, thus adjusting dynamic processing of visual information under varying light conditions. SIGNIFICANCE STATEMENT Drosophila photoreceptors exhibit high temporal resolution as manifested in frequency response to oscillating light of high frequency (≤∼100 Hz). Light rearing conditions modulate the maximal frequency detected by photoreceptors, thus enabling them to maintain high sensitivity to light and high temporal resolution. However, the precise mechanisms for this process are not fully understood. Here, we show by combination of biochemistry and in vivo electrophysiology that transient receptor potential (TRP) channel dephosphorylation at a specific site is a fast, light-activated and Ca2+-dependent regulatory process. TRP dephosphorylation affects the detection limit of oscillating light according to the adaptation state of the photoreceptor cells by shifting the detection limit to higher frequencies upon light adaptation. This novel mechanism thus adjusts dynamic processing of visual information under varying light conditions. PMID:28314815
Preparing Specialized Undergraduate Pilot Training Graduates for F-35A Training
2010-06-11
the two sides of this argument and analyze the new data acquired from an F-22A lead-in program and the fact that the F-35A has started flying with...flight operations under Instrument or Visual Flight Rules to include day / night IFR operations in the terminal and enroute environment. Have limited
Air-to-Air Visual Acquisition Handbook
1991-11-27
expending a normal amount of effort on visual search. * A target aicraft begins to approach. Itisa - IACT= 1 Piper PA-28 flying on an unaccelerated...output for SEEL. 17 TABLE 4.1 AIRCRAFT TYPES USED IN SEEI AX AY AZ IACT AIRCRAFT TYPE NAME (sq. ft.) (sq. ft.) (sq. ft.) 1 Piper PA-28 PA28 35 85 260 2...VSUB 180.0 FOVL -120.00 T2 12.0 VTAR 130.0 FOVR 90.0 D1 0.00 IACT 1.00 D2 0.00 XANG 90.0 * - initial value (before iteration) ROW VARIABLE: BETL is
Flattening maps for the visualization of multibranched vessels.
Zhu, Lei; Haker, Steven; Tannenbaum, Allen
2005-02-01
In this paper, we present two novel algorithms which produce flattened visualizations of branched physiological surfaces, such as vessels. The first approach is a conformal mapping algorithm based on the minimization of two Dirichlet functionals. From a triangulated representation of vessel surfaces, we show how the algorithm can be implemented using a finite element technique. The second method is an algorithm which adjusts the conformal mapping to produce a flattened representation of the original surface while preserving areas. This approach employs the theory of optimal mass transport. Furthermore, a new way of extracting center lines for vessel fly-throughs is provided.
Flattening Maps for the Visualization of Multibranched Vessels
Zhu, Lei; Haker, Steven; Tannenbaum, Allen
2013-01-01
In this paper, we present two novel algorithms which produce flattened visualizations of branched physiological surfaces, such as vessels. The first approach is a conformal mapping algorithm based on the minimization of two Dirichlet functionals. From a triangulated representation of vessel surfaces, we show how the algorithm can be implemented using a finite element technique. The second method is an algorithm which adjusts the conformal mapping to produce a flattened representation of the original surface while preserving areas. This approach employs the theory of optimal mass transport. Furthermore, a new way of extracting center lines for vessel fly-throughs is provided. PMID:15707245
NASA Technical Reports Server (NTRS)
Hill, Michael A.; Haering, Edward A., Jr.
2017-01-01
The Background Oriented Schlieren using Celestial Objects series of flights was undertaken in the spring of 2016 at National Aeronautics and Space Administration Armstrong Flight Research Center to further develop and improve a flow visualization technique which can be performed from the ground upon flying aircraft. Improved hardware and imaging techniques from previous schlieren tests were investigated. A United States Air Force T-38C and NASA B200 King Air aircraft were imaged eclipsing the sun at ranges varying from 2 to 6 nautical miles, at subsonic and supersonic speeds.
Chilled packing systems for fruit flies (Diptera: Tephritidae) in the sterile insect technique.
Hernández, Emilio; Escobar, Arseny; Bravo, Bigail; Montoya, Pablo
2010-01-01
We evaluated three packing systems (PARC boxes, "GT" screen towers and "MX" screen towers) for the emergence and sexual maturation of sterile fruit flies, at three adult fly densities (1, 1.2 and 1.3 fly/cm²) and three food types. At the lowest density, results showed no significant differences in the longevity and flight ability of adult Anastrepha ludens (Loew) and Anastrepha obliqua Macquart among the three packing systems. Higher densities resulted in a decrease in these parameters. In the evaluation of the three food types, no significant differences were found either on longevity or flight ability of A. ludens. However, the greatest longevity for both sexes A. obliqua was obtained with commercial powdered Mb® and the mix of sugar, protein and corn starch on paper (SPCP) food types. The highest value for flight ability in A. obliqua males was obtained with powdered Mb® and SPCP food types, and for females with Mb® powdered food. Our data indicated that GT and MX screen tower packing systems are an alternative to the PARC boxes, since they were suitable for adult fly sexual maturation without any harm to their longevity or flight ability. The tested foods were equivalent in both fruit fly species, with the exception of the agar type for A. obliqua, which yielded the lowest biological parameters evaluated. Our results contribute to the application of new methods for the packing and release of sterile flies in large-scale programs.
Fly-by-light flight control system technology development plan
NASA Technical Reports Server (NTRS)
Chakravarty, A.; Berwick, J. W.; Griffith, D. M.; Marston, S. E.; Norton, R. L.
1990-01-01
The results of a four-month, phased effort to develop a Fly-by-Light Technology Development Plan are documented. The technical shortfalls for each phase were identified and a development plan to bridge the technical gap was developed. The production configuration was defined for a 757-type airplane, but it is suggested that the demonstration flight be conducted on the NASA Transport Systems Research Vehicle. The modifications required and verification and validation issues are delineated in this report. A detailed schedule for the phased introduction of fly-by-light system components has been generated. It is concluded that a fiber-optics program would contribute significantly toward developing the required state of readiness that will make a fly-by-light control system not only cost effective but reliable without mitigating the weight and high-energy radio frequency related benefits.
Preliminary Results of NASA's First Autonomous Formation Flying Experiment: Earth Observing-1 (EO-1)
NASA Technical Reports Server (NTRS)
Folta, David; Hawkins, Albin
2001-01-01
NASA's first autonomous formation flying mission is completing a primary goal of demonstrating an advanced technology called enhanced formation flying. To enable this technology, the Guidance, Navigation, and Control center at the Goddard Space Flight Center has implemented an autonomous universal three-axis formation flying algorithm in executive flight code onboard the New Millennium Program's (NMP) Earth Observing-1 (EO-1) spacecraft. This paper describes the mathematical background of the autonomous formation flying algorithm and the onboard design and presents the preliminary validation results of this unique system. Results from functionality assessment and autonomous maneuver control are presented as comparisons between the onboard EO-1 operational autonomous control system called AutoCon(tm), its ground-based predecessor, and a stand-alone algorithm.
Fruit fly scale robots can hover longer with flapping wings than with spinning wings.
Hawkes, Elliot W; Lentink, David
2016-10-01
Hovering flies generate exceptionally high lift, because their wings generate a stable leading edge vortex. Micro flying robots with a similar wing design can generate similar high lift by either flapping or spinning their wings. While it requires less power to spin a wing, the overall efficiency depends also on the actuator system driving the wing. Here, we present the first holistic analysis to calculate how long a fly-inspired micro robot can hover with flapping versus spinning wings across scales. We integrate aerodynamic data with data-driven scaling laws for actuator, electronics and mechanism performance from fruit fly to hummingbird scales. Our analysis finds that spinning wings driven by rotary actuators are superior for robots with wingspans similar to hummingbirds, yet flapping wings driven by oscillatory actuators are superior at fruit fly scale. This crossover is driven by the reduction in performance of rotary compared with oscillatory actuators at smaller scale. Our calculations emphasize that a systems-level analysis is essential for trading-off flapping versus spinning wings for micro flying robots. © 2016 The Author(s).
Fruit fly scale robots can hover longer with flapping wings than with spinning wings
Lentink, David
2016-01-01
Hovering flies generate exceptionally high lift, because their wings generate a stable leading edge vortex. Micro flying robots with a similar wing design can generate similar high lift by either flapping or spinning their wings. While it requires less power to spin a wing, the overall efficiency depends also on the actuator system driving the wing. Here, we present the first holistic analysis to calculate how long a fly-inspired micro robot can hover with flapping versus spinning wings across scales. We integrate aerodynamic data with data-driven scaling laws for actuator, electronics and mechanism performance from fruit fly to hummingbird scales. Our analysis finds that spinning wings driven by rotary actuators are superior for robots with wingspans similar to hummingbirds, yet flapping wings driven by oscillatory actuators are superior at fruit fly scale. This crossover is driven by the reduction in performance of rotary compared with oscillatory actuators at smaller scale. Our calculations emphasize that a systems-level analysis is essential for trading-off flapping versus spinning wings for micro flying robots. PMID:27707903
A Miniaturized Video System for Monitoring Drosophila Behavior
NASA Technical Reports Server (NTRS)
Bhattacharya, Sharmila; Inan, Omer; Kovacs, Gregory; Etemadi, Mozziyar; Sanchez, Max; Marcu, Oana
2011-01-01
Long-term spaceflight may induce a variety of harmful effects in astronauts, resulting in altered motor and cognitive behavior. The stresses experienced by humans in space - most significantly weightlessness (microgravity) and cosmic radiation - are difficult to accurately simulate on Earth. In fact, prolonged and concomitant exposure to microgravity and cosmic radiation can only be studied in space. Behavioral studies in space have focused on model organisms, including Drosophila melanogaster. Drosophila is often used due to its short life span and generational cycle, small size, and ease of maintenance. Additionally, the well-characterized genetics of Drosophila behavior on Earth can be applied to the analysis of results from spaceflights, provided that the behavior in space is accurately recorded. In 2001, the BioExplorer project introduced a low-cost option for researchers: the small satellite. While this approach enabled multiple inexpensive launches of biological experiments, it also imposed stringent restrictions on the monitoring systems in terms of size, mass, data bandwidth, and power consumption. Suggested parameters for size are on the order of 100 mm3 and 1 kg mass for the entire payload. For Drosophila behavioral studies, these engineering requirements are not met by commercially available systems. One system that does meet many requirements for behavioral studies in space is the actimeter. Actimeters use infrared light gates to track the number of times a fly crosses a boundary within a small container (3x3x40 mm). Unfortunately, the apparatus needed to monitor several flies at once would be larger than the capacity of the small satellite. A system is presented, which expands on the actimeter approach to achieve a highly compact, low-power, ultra-low bandwidth solution for simultaneous monitoring of the behavior of multiple flies in space. This also provides a simple, inexpensive alternative to the current systems for monitoring Drosophila populations in terrestrial experiments, and could be especially useful in field experiments in remote locations. Two practical limitations of the system should be noted: first, only walking flies can be observed - not flying - and second, although it enables population studies, tracking individual flies within the population is not currently possible. The system used video recording and an analog circuit to extract the average light changes as a function of time. Flies were held in a 5-cm diameter Petri dish and illuminated from below by a uniform light source. A miniature, monochrome CMOS (complementary metal-oxide semiconductor) video camera imaged the flies. This camera had automatic gain control, and this did not affect system performance. The camera was positioned 5-7 cm above the Petri dish such that the imaging area was 2.25 sq cm. With this basic setup, still images and continuous video of 15 flies at one time were obtained. To reduce the required data bandwidth by several orders of magnitude, a band-pass filter (0.3-10 Hz) circuit compressed the video signal and extracted changes in image luminance over time. The raw activity signal output of this circuit was recorded on a computer and digitally processed to extract the fly movement "events" from the waveform. These events corresponded to flies entering and leaving the image and were used for extracting activity parameters such as inter-event duration. The efficacy of the system in quantifying locomotor activity was evaluated by varying environmental temperature, then measuring the activity level of the flies.
Can invertebrates see the e-vector of polarization as a separate modality of light?
Labhart, Thomas
2016-12-15
The visual world is rich in linearly polarized light stimuli, which are hidden from the human eye. But many invertebrate species make use of polarized light as a source of valuable visual information. However, exploiting light polarization does not necessarily imply that the electric (e)-vector orientation of polarized light can be perceived as a separate modality of light. In this Review, I address the question of whether invertebrates can detect specific e-vector orientations in a manner similar to that of humans perceiving spectral stimuli as specific hues. To analyze e-vector orientation, the signals of at least three polarization-sensitive sensors (analyzer channels) with different e-vector tuning axes must be compared. The object-based, imaging polarization vision systems of cephalopods and crustaceans, as well as the water-surface detectors of flying backswimmers, use just two analyzer channels. Although this excludes the perception of specific e-vector orientations, a two-channel system does provide a coarse, categoric analysis of polarized light stimuli, comparable to the limited color sense of dichromatic, 'color-blind' humans. The celestial compass of insects employs three or more analyzer channels. However, that compass is multimodal, i.e. e-vector information merges with directional information from other celestial cues, such as the solar azimuth and the spectral gradient in the sky, masking e-vector information. It seems that invertebrate organisms take no interest in the polarization details of visual stimuli, but polarization vision grants more practical benefits, such as improved object detection and visual communication for cephalopods and crustaceans, compass readings to traveling insects, or the alert 'water below!' to water-seeking bugs. © 2016. Published by The Company of Biologists Ltd.
Can invertebrates see the e-vector of polarization as a separate modality of light?
2016-01-01
ABSTRACT The visual world is rich in linearly polarized light stimuli, which are hidden from the human eye. But many invertebrate species make use of polarized light as a source of valuable visual information. However, exploiting light polarization does not necessarily imply that the electric (e)-vector orientation of polarized light can be perceived as a separate modality of light. In this Review, I address the question of whether invertebrates can detect specific e-vector orientations in a manner similar to that of humans perceiving spectral stimuli as specific hues. To analyze e-vector orientation, the signals of at least three polarization-sensitive sensors (analyzer channels) with different e-vector tuning axes must be compared. The object-based, imaging polarization vision systems of cephalopods and crustaceans, as well as the water-surface detectors of flying backswimmers, use just two analyzer channels. Although this excludes the perception of specific e-vector orientations, a two-channel system does provide a coarse, categoric analysis of polarized light stimuli, comparable to the limited color sense of dichromatic, ‘color-blind’ humans. The celestial compass of insects employs three or more analyzer channels. However, that compass is multimodal, i.e. e-vector information merges with directional information from other celestial cues, such as the solar azimuth and the spectral gradient in the sky, masking e-vector information. It seems that invertebrate organisms take no interest in the polarization details of visual stimuli, but polarization vision grants more practical benefits, such as improved object detection and visual communication for cephalopods and crustaceans, compass readings to traveling insects, or the alert ‘water below!’ to water-seeking bugs. PMID:27974532
Virtual reality in the operating room of the future.
Müller, W; Grosskopf, S; Hildebrand, A; Malkewitz, R; Ziegler, R
1997-01-01
In cooperation with the Max-Delbrück-Centrum/Robert-Rössle-Klinik (MDC/RRK) in Berlin, the Fraunhofer Institute for Computer Graphics is currently designing and developing a scenario for the operating room of the future. The goal of this project is to integrate new analysis, visualization and interaction tools in order to optimize and refine tumor diagnostics and therapy in combination with laser technology and remote stereoscopic video transfer. Hence, a human 3-D reference model is reconstructed using CT, MR, and anatomical cryosection images from the National Library of Medicine's Visible Human Project. Applying segmentation algorithms and surface-polygonization methods a 3-D representation is obtained. In addition, a "fly-through" the virtual patient is realized using 3-D input devices (data glove, tracking system, 6-DOF mouse). In this way, the surgeon can experience really new perspectives of the human anatomy. Moreover, using a virtual cutting plane any cut of the CT volume can be interactively placed and visualized in realtime. In conclusion, this project delivers visions for the application of effective visualization and VR systems. Commonly known as Virtual Prototyping and applied by the automotive industry long ago, this project shows, that the use of VR techniques can also prototype an operating room. After evaluating design and functionality of the virtual operating room, MDC plans to build real ORs in the near future. The use of VR techniques provides a more natural interface for the surgeon in the OR (e.g., controlling interactions by voice input). Besides preoperative planning future work will focus on supporting the surgeon in performing surgical interventions. An optimal synthesis of real and synthetic data, and the inclusion of visual, aural, and tactile senses in virtual environments can meet these requirements. This Augmented Reality could represent the environment for the surgeons of tomorrow.
Helicopter flight simulation motion platform requirements
NASA Astrophysics Data System (ADS)
Schroeder, Jeffery Allyn
Flight simulators attempt to reproduce in-flight pilot-vehicle behavior on the ground. This reproduction is challenging for helicopter simulators, as the pilot is often inextricably dependent on external cues for pilot-vehicle stabilization. One important simulator cue is platform motion; however, its required fidelity is unknown. To determine the required motion fidelity, several unique experiments were performed. A large displacement motion platform was used that allowed pilots to fly tasks with matched motion and visual cues. Then, the platform motion was modified to give cues varying from full motion to no motion. Several key results were found. First, lateral and vertical translational platform cues had significant effects on fidelity. Their presence improved performance and reduced pilot workload. Second, yaw and roll rotational platform cues were not as important as the translational platform cues. In particular, the yaw rotational motion platform cue did not appear at all useful in improving performance or reducing workload. Third, when the lateral translational platform cue was combined with visual yaw rotational cues, pilots believed the platform was rotating when it was not. Thus, simulator systems can be made more efficient by proper combination of platform and visual cues. Fourth, motion fidelity specifications were revised that now provide simulator users with a better prediction of motion fidelity based upon the frequency responses of their motion control laws. Fifth, vertical platform motion affected pilot estimates of steady-state altitude during altitude repositionings. This refutes the view that pilots estimate altitude and altitude rate in simulation solely from visual cues. Finally, the combined results led to a general method for configuring helicopter motion systems and for developing simulator tasks that more likely represent actual flight. The overall results can serve as a guide to future simulator designers and to today's operators.
NASA Astrophysics Data System (ADS)
Yudhi Irwanto, Herma
2018-02-01
The development of autonomous controller system that is specially used in our high speed UAV, it’s call RKX-200EDF/TJ controlled vehicle needs to be continued as a step to mastery and to developt control system of LAPAN’s satellite launching rocket. The weakness of the existing control system in this high speed UAV needs to be repaired and replaced using the autonomous controller system. Conversion steps for ready-to-fly system involved controlling X tail fin, adjusting auto take off procedure by adding X axis sensor, procedure of way points reading and process of measuring distance and heading to the nearest way point, developing user-friendly ground station, and adding tools for safety landing. The development of this autonomous controller system also covered a real flying test in Pandanwangi, Lumajang in November 2016. Unfortunately, the flying test was not successful because the booster rocket was blown right after burning. However, the system could record the event and demonstrated that the controller system had worked according to plan.
Control of free-flying space robot manipulator systems
NASA Technical Reports Server (NTRS)
Cannon, Robert H., Jr.
1977-01-01
To accelerate the development of multi-armed, free-flying satellite manipulators, a fixed-base cooperative manipulation facility is being developed. The work performed on multiple arm cooperation on a free-flying robot is summarized. Research is also summarized on global navigation and control of free-flying space robots. The Locomotion Enhancement via Arm Pushoff (LEAP) approach is described and progress to date is presented.
Helmet-mounted pilot night vision systems: Human factors issues
NASA Technical Reports Server (NTRS)
Hart, Sandra G.; Brickner, Michael S.
1989-01-01
Helmet-mounted displays of infrared imagery (forward-looking infrared (FLIR)) allow helicopter pilots to perform low level missions at night and in low visibility. However, pilots experience high visual and cognitive workload during these missions, and their performance capabilities may be reduced. Human factors problems inherent in existing systems stem from three primary sources: the nature of thermal imagery; the characteristics of specific FLIR systems; and the difficulty of using FLIR system for flying and/or visually acquiring and tracking objects in the environment. The pilot night vision system (PNVS) in the Apache AH-64 provides a monochrome, 30 by 40 deg helmet-mounted display of infrared imagery. Thermal imagery is inferior to television imagery in both resolution and contrast ratio. Gray shades represent temperatures differences rather than brightness variability, and images undergo significant changes over time. The limited field of view, displacement of the sensor from the pilot's eye position, and monocular presentation of a bright FLIR image (while the other eye remains dark-adapted) are all potential sources of disorientation, limitations in depth and distance estimation, sensations of apparent motion, and difficulties in target and obstacle detection. Insufficient information about human perceptual and performance limitations restrains the ability of human factors specialists to provide significantly improved specifications, training programs, or alternative designs. Additional research is required to determine the most critical problem areas and to propose solutions that consider the human as well as the development of technology.
Dong, Han; Sharma, Diksha; Badano, Aldo
2014-12-01
Monte Carlo simulations play a vital role in the understanding of the fundamental limitations, design, and optimization of existing and emerging medical imaging systems. Efforts in this area have resulted in the development of a wide variety of open-source software packages. One such package, hybridmantis, uses a novel hybrid concept to model indirect scintillator detectors by balancing the computational load using dual CPU and graphics processing unit (GPU) processors, obtaining computational efficiency with reasonable accuracy. In this work, the authors describe two open-source visualization interfaces, webmantis and visualmantis to facilitate the setup of computational experiments via hybridmantis. The visualization tools visualmantis and webmantis enable the user to control simulation properties through a user interface. In the case of webmantis, control via a web browser allows access through mobile devices such as smartphones or tablets. webmantis acts as a server back-end and communicates with an NVIDIA GPU computing cluster that can support multiuser environments where users can execute different experiments in parallel. The output consists of point response and pulse-height spectrum, and optical transport statistics generated by hybridmantis. The users can download the output images and statistics through a zip file for future reference. In addition, webmantis provides a visualization window that displays a few selected optical photon path as they get transported through the detector columns and allows the user to trace the history of the optical photons. The visualization tools visualmantis and webmantis provide features such as on the fly generation of pulse-height spectra and response functions for microcolumnar x-ray imagers while allowing users to save simulation parameters and results from prior experiments. The graphical interfaces simplify the simulation setup and allow the user to go directly from specifying input parameters to receiving visual feedback for the model predictions.
Web-Based Geospatial Visualization of GPM Data with CesiumJS
NASA Technical Reports Server (NTRS)
Lammers, Matt
2018-01-01
Advancements in the capabilities of JavaScript frameworks and web browsing technology have made online visualization of large geospatial datasets such as those coming from precipitation satellites viable. These data benefit from being visualized on and above a three-dimensional surface. The open-source JavaScript framework CesiumJS (http://cesiumjs.org), developed by Analytical Graphics, Inc., leverages the WebGL protocol to do just that. This presentation will describe how CesiumJS has been used in three-dimensional visualization products developed as part of the NASA Precipitation Processing System (PPS) STORM data-order website. Existing methods of interacting with Global Precipitation Measurement (GPM) Mission data primarily focus on two-dimensional static images, whether displaying vertical slices or horizontal surface/height-level maps. These methods limit interactivity with the robust three-dimensional data coming from the GPM core satellite. Integrating the data with CesiumJS in a web-based user interface has allowed us to create the following products. We have linked with the data-order interface an on-the-fly visualization tool for any GPM/partner satellite orbit. A version of this tool also focuses on high-impact weather events. It enables viewing of combined radar and microwave-derived precipitation data on mobile devices and in a way that can be embedded into other websites. We also have used CesiumJS to visualize a method of integrating gridded precipitation data with modeled wind speeds that animates over time. Emphasis in the presentation will be placed on how a variety of technical methods were used to create these tools, and how the flexibility of the CesiumJS framework facilitates creative approaches to interact with the data.
Bonaccorso, Frank; Montoya-Aiona, Kristina; Pinzari, Corinna A.; Todd, Christopher M.
2016-01-01
We examine altitudinal movements involving unusual use of caves by Hawaiian hoary bats, Lasiurus cinereus semotus, during winter and spring in the Mauna Loa Forest Reserve (MLFR), Hawai‘i Island. Acoustic detection of hoary bat vocalizations, were recorded with regularity outside 13 lava tube cave entrances situated between 2,200 to 3,600 m asl from November 2012 to April 2013. Vocalizations were most numerous in November and December with the number of call events and echolocation pulses decreasing through the following months. Bat activity was positively correlated with air temperature and negatively correlated with wind speed. Visual searches found no evidence of hibernacula nor do Hawaiian hoary bats appear to shelter by day in these caves. Nevertheless, bats fly deep into caves as evidenced by numerous carcasses found in cave interiors. The occurrence of feeding buzzes around cave entrances and visual observations of bats flying in acrobatic fashion in cave interiors point to the use of these spaces as foraging sites. Peridroma moth species (Noctuidae), the only abundant nocturnal, flying insect sheltering in large numbers in rock rubble and on cave walls in the MLFR, apparently serve as the principal prey attracting hoary bats during winter to lava tube caves in the upper MLFR. Caves above 3,000 m on Mauna Loa harbor temperatures suitable for Pseudogymnoascus destructansfungi, the causative agent of White-nose Syndrome that is highly lethal to some species of North American cave-dwelling bats. We discuss the potential for White-nose Syndrome to establish and affect Hawaiian hoary bats.
Extractability, plant yield and toxicity thresholds for boron in compost
DOE Office of Scientific and Technical Information (OSTI.GOV)
Brinton, W.F.; Evans, E.; Blewett, C.
Boron (B) is a trace element essential to crop growth in small soil concentrations (0.2-1.5ppm), yet may produce plant toxicity symptoms readily as the amount in the soil solution increases over 2ppm. Our study examined commercial compost made with coal fly-ash used to prepare growing media for cultivars of varying sensitivity (corn, beans, cucumber, peas). We examined total vs. extractable boron content and relate final visual symptoms of B-toxicity to yields and tissue concentrations. Visual toxicity effects included tip burn (corn), leaf mottling and necrosis (beans and peas) and leaf mottling and cupping (cucumbers). Fly ash added to compost increasedmore » hot-water soluble (HWS) B in proportion to rate and in dependence on pH, with 30% and 10% of total-B expressed as HWS-B at a media pH of 6 and 7.5, respectively. Biomass for bean and cucumber was significantly reduced by 45 to 55%, respectively, by addition of 33% fly-ash compost to growing media (28ppm total-B) while plant tissue-B increased by 6- to 4-fold, respectively. Economic yield depressions in compost media are evident for all crops and appeared at levels of HWS-B in compost media exceeding 5 ppm. The study underscores the need for careful management of exogenous factors that may be present in composts and suggests detailed understanding of media-pH and cultivar preferences may be required in preparation of growing media in order to reduce potential negative growth effects.« less
Analysis of rocket flight stability based on optical image measurement
NASA Astrophysics Data System (ADS)
Cui, Shuhua; Liu, Junhu; Shen, Si; Wang, Min; Liu, Jun
2018-02-01
Based on the abundant optical image measurement data from the optical measurement information, this paper puts forward the method of evaluating the rocket flight stability performance by using the measurement data of the characteristics of the carrier rocket in imaging. On the basis of the method of measuring the characteristics of the carrier rocket, the attitude parameters of the rocket body in the coordinate system are calculated by using the measurements data of multiple high-speed television sets, and then the parameters are transferred to the rocket body attack angle and it is assessed whether the rocket has a good flight stability flying with a small attack angle. The measurement method and the mathematical algorithm steps through the data processing test, where you can intuitively observe the rocket flight stability state, and also can visually identify the guidance system or failure analysis.
Piñero, Jaime C.; Mau, Ronald F. L.; Vargas, Roger I.
2010-01-01
Bait stations represent an environmentally friendly attract-and-kill approach to fruit fly population suppression. Recently a novel, visually attractive, rain-fast bait station was developed in Hawaii for potential use against multiple species of pestiferous fruit flies. Here, we compared the efficacy of GF-120 NF Naturalyte Fruit Fly Bait applied either as foliar sprays or onto bait stations in reducing female oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), population density and level of fruit infestation in commercial papaya orchards in Hawaii. Trapping and infestation data were used as indicators of the effectiveness of the two bait application methods. For the first 10 weeks of the study, captures of female B. dorsalis in monitoring traps were significantly greater in control plots than in plots treated with foliar sprays or bait stations. Six weeks after the first bait spray, incidence of infestation (i.e. number of fruit with one or more B. dorsalis larvae) of quarter to half-ripe papaya fruit was reduced by 71.4% and 63.1% for plots with bait stations and foliar sprays, respectively, as compared to control plots. Twelve weeks after first spray, incidence of infestation was reduced by only 54.5% and 45.4% for plots with bait stations and foliar sprays, respectively, as compared to control plots. About 42% less GF-120 was used in orchard plots with bait stations compared to those subject to foliar sprays. The impact of field sanitation on the outcome is also discussed. The results indicate that bait stations can provide a simple, efficient, and economical method of applying insecticidal baits to control fruit flies and a safer alternative to foliar sprays. PMID:21067423
1976-05-01
film airflow anemometry used for vortex measurements in the series of tests is described in reference 6. However, there were two major differences in...LOCKHEED 11011 TRAILING VORTEX SYSTEM USING TOWER FLY-BY TECHNIQUE Leo J. fiarodz ,OtTt4V MAY 1976 FINAL REPORT D k ■?tp r~ "ft UElaibu u...THE LOCKHEED L1011 TRAILING VORTEX SYSTEM USING TOWER FLY-BY TECHNIQUE 7. Authc Leo J. Garodz 9. Performing Orgoni lotion Nome ond Address
Context generalization in Drosophila visual learning requires the mushroom bodies
NASA Astrophysics Data System (ADS)
Liu, Li; Wolf, Reinhard; Ernst, Roman; Heisenberg, Martin
1999-08-01
The world is permanently changing. Laboratory experiments on learning and memory normally minimize this feature of reality, keeping all conditions except the conditioned and unconditioned stimuli as constant as possible. In the real world, however, animals need to extract from the universe of sensory signals the actual predictors of salient events by separating them from non-predictive stimuli (context). In principle, this can be achieved ifonly those sensory inputs that resemble the reinforcer in theirtemporal structure are taken as predictors. Here we study visual learning in the fly Drosophila melanogaster, using a flight simulator,, and show that memory retrieval is, indeed, partially context-independent. Moreover, we show that the mushroom bodies, which are required for olfactory but not visual or tactile learning, effectively support context generalization. In visual learning in Drosophila, it appears that a facilitating effect of context cues for memory retrieval is the default state, whereas making recall context-independent requires additional processing.
Taste and pheromone perception in the fruit fly Drosophila melanogaster.
Ebbs, Michelle L; Amrein, Hubert
2007-08-01
Taste is an essential sense for detection of nutrient-rich food and avoidance of toxic substances. The Drosophila melanogaster gustatory system provides an excellent model to study taste perception and taste-elicited behaviors. "The fly" is unique in the animal kingdom with regard to available experimental tools, which include a wide repertoire of molecular-genetic analyses (i.e., efficient production of transgenics and gene knockouts), elegant behavioral assays, and the possibility to conduct electrophysiological investigations. In addition, fruit flies, like humans, recognize sugars as a food source, but avoid bitter tasting substances that are often toxic to insects and mammals alike. This paper will present recent research progress in the field of taste and contact pheromone perception in the fruit fly. First, we shall describe the anatomical properties of the Drosophila gustatory system and survey the family of taste receptors to provide an appropriate background. We shall then review taste and pheromone perception mainly from a molecular genetic perspective that includes behavioral, electrophysiological and imaging analyses of wild type flies and flies with genetically manipulated taste cells. Finally, we shall provide an outlook of taste research in this elegant model system for the next few years.
Bednar, A J; Averett, D E; Seiter, J M; Lafferty, B; Jones, W T; Hayes, C A; Chappell, M A; Clarke, J U; Steevens, J A
2013-09-01
A storage-pond dike failure occurred on December 22, 2008 at the Tennessee Valley Authority Kingston Fossil Plant resulting in the release of over 4million cubic meters (5million cubic yards) of fly ash. Approximately half of the released ash was deposited in the main channel of the Emory River, Tennessee, USA. Remediation efforts of the Emory River focused on hydraulic dredging, as well as mechanical excavation in targeted areas. However, agitation of the submerged fly ash during hydraulic dredging introduces river water into the fly ash material, which could promote dissolution and desorption of metals from the solid fly ash material. Furthermore, aeration of the dredge slurry could alter the redox state of metals in the fly ash material and thereby change their sorption, mobility, and toxicity properties. The research presented here focuses on the concentrations and speciation of metals during the fly ash recovery from the Emory River. Our results indicate that arsenite [As(III)] released from the fly ash material during dredging was slowly oxidized to arsenate [As(V)] in the slurry recovery system with subsequent removal through precipitation or sorption reactions with suspended fly ash material. Concentrations of other dissolved metals, including iron and manganese, also generally decreased in the ash recovery system prior to water discharge back to the river. Published by Elsevier Ltd.
Sajó, Ráchel; Liliom, Károly; Muskotál, Adél; Klein, Agnes; Závodszky, Péter; Vonderviszt, Ferenc; Dobó, József
2014-11-01
Flagella, the locomotion organelles of bacteria, extend from the cytoplasm to the cell exterior. External flagellar proteins are synthesized in the cytoplasm and exported by the flagellar type III secretion system. Soluble components of the flagellar export apparatus, FliI, FliH, and FliJ, have been implicated to carry late export substrates in complex with their cognate chaperones from the cytoplasm to the export gate. The importance of the soluble components in the delivery of the three minor late substrates FlgK, FlgL (hook-filament junction) and FliD (filament-cap) has been convincingly demonstrated, but their role in the transport of the major filament component flagellin (FliC) is still unclear. We have used continuous ATPase activity measurements and quartz crystal microbalance (QCM) studies to characterize interactions between the soluble export components and flagellin or the FliC:FliS substrate-chaperone complex. As controls, interactions between soluble export component pairs were characterized providing Kd values. FliC or FliC:FliS did not influence the ATPase activity of FliI alone or in complex with FliH and/or FliJ suggesting lack of interaction in solution. Immobilized FliI, FliH, or FliJ did not interact with FliC or FliC:FliS detected by QCM. The lack of interaction in the fluid phase between FliC or FliC:FliS and the soluble export components, in particular with the ATPase FliI, suggests that cells use different mechanisms for the export of late minor substrates, and the major substrate, FliC. It seems that the abundantly produced flagellin does not require the assistance of the soluble export components to efficiently reach the export gate. Copyright © 2014 Elsevier B.V. All rights reserved.
Wu, Ming; Nern, Aljoscha; Williamson, W Ryan; Morimoto, Mai M; Reiser, Michael B; Card, Gwyneth M; Rubin, Gerald M
2016-01-01
Visual projection neurons (VPNs) provide an anatomical connection between early visual processing and higher brain regions. Here we characterize lobula columnar (LC) cells, a class of Drosophila VPNs that project to distinct central brain structures called optic glomeruli. We anatomically describe 22 different LC types and show that, for several types, optogenetic activation in freely moving flies evokes specific behaviors. The activation phenotypes of two LC types closely resemble natural avoidance behaviors triggered by a visual loom. In vivo two-photon calcium imaging reveals that these LC types respond to looming stimuli, while another type does not, but instead responds to the motion of a small object. Activation of LC neurons on only one side of the brain can result in attractive or aversive turning behaviors depending on the cell type. Our results indicate that LC neurons convey information on the presence and location of visual features relevant for specific behaviors. DOI: http://dx.doi.org/10.7554/eLife.21022.001 PMID:28029094
A comparison of the Space Station version of ASTROMAG with two free-flyer versions
NASA Astrophysics Data System (ADS)
Green, M. A.
1992-06-01
The Space Station version of ASTROMAG is compared with free-flyer versions of ASTROMAG which could fly on an Atlas 2a rocket and a Delta rocket. Launch with either free-flyer imposes severe weight limits on the magnet and its cryogenic system. Both versions of ASTROMAG magnet which fly on free-flying satellites do not have to be charged more than once during the mission. This permits one to simplify the charging system and the cryogenic system. The helium 2 pump loop which supplies helium to the gas cooled electrical leads can be eliminated in both of the free-flyer versions of the ASTROMAG magnet. The superconducting dipole moment correction coils which are necessary for the magnet to operate on a free-flying satellite are described.
NASA Astrophysics Data System (ADS)
Yuren, Wang; Fang, Shao; Weiping, Sun; Xioujuan, Li; Suning, Tian; Hongyan, Li
1989-06-01
When a heavy-calibre gun is fired and a projectite is flying near the gun muzzle, velocity of the projectile is very high and firing process is accompanying with strong muzzle flash. So taking the picture of the attitudes of flying projectile at the gun muzzle is very difficult. "YDS speed Photography System" developed by our group can take the framing pictures of the attitudes of the projectile and prevent them from flash confusing at the muzzle. Since framing depends on sequential pulse of the laser and the width of the putse is very narrow, therefore the exposure time is very short and photos of high-velocity flying body taken are very clear. This paper Introduces configuration and operation principle of "YDS laser High-speed Photography System" and the fuctions of the devices in this system In addition, some experimental results are briefly introduced.
Extending helicopter operations to meet future integrated transportation needs.
Stanton, Neville A; Plant, Katherine L; Roberts, Aaron P; Harvey, Catherine; Thomas, T Glyn
2016-03-01
Helicopters have the potential to be an integral part of the future transport system. They offer a means of rapid transit in an overly populated transport environment. However, one of the biggest limitations on rotary wing flight is their inability to fly in degraded visual conditions in the critical phases of approach and landing. This paper presents a study that developed and evaluated a Head up Display (HUD) to assist rotary wing pilots by extending landing to degraded visual conditions. The HUD was developed with the assistance of the Cognitive Work Analysis method as an approach for analysing the cognitive work of landing the helicopter. The HUD was tested in a fixed based flight simulator with qualified helicopter pilots. A qualitative analysis to assess situation awareness and workload found that the HUD enabled safe landing in degraded conditions whilst simultaneously enhancing situation awareness and reducing workload. Continued development in this area has the potential to extend the operational capability of helicopters in the future. Copyright © 2015 Elsevier Ltd and The Ergonomics Society. All rights reserved.
Targeting of Drosophila Rhodopsin Requires Helix 8 but Not the Distal C-Terminus
Kock, Ines; Bulgakova, Natalia A.; Knust, Elisabeth; Sinning, Irmgard; Panneels, Valérie
2009-01-01
Background The fundamental role of the light receptor rhodopsin in visual function and photoreceptor cell development has been widely studied. Proper trafficking of rhodopsin to the photoreceptor membrane is of great importance. In human, mutations in rhodopsin involving its intracellular mislocalization, are the most frequent cause of autosomal dominant Retinitis Pigmentosa, a degenerative retinal pathology characterized by progressive blindness. Drosophila is widely used as an animal model in visual and retinal degeneration research. So far, little is known about the requirements for proper rhodopsin targeting in Drosophila. Methodology/Principal Findings Different truncated fly-rhodopsin Rh1 variants were expressed in the eyes of Drosophila and their localization was analyzed in vivo or by immunofluorescence. A mutant lacking the last 23 amino acids was found to properly localize in the rhabdomeres, the light-sensing organelle of the photoreceptor cells. This constitutes a major difference to trafficking in vertebrates, which involves a conserved QVxPA motif at the very C-terminus. Further truncations of Rh1 indicated that proper localization requires the last amino acid residues of a region called helix 8 following directly the last transmembrane domain. Interestingly, the very C-terminus of invertebrate visual rhodopsins is extremely variable but helix 8 shows conserved amino acid residues that are not conserved in vertebrate homologs. Conclusions/Significance Despite impressive similarities in the folding and photoactivation of vertebrate and invertebrate visual rhodopsins, a striking difference exists between mammalian and fly rhodopsins in their requirements for proper targeting. Most importantly, the distal part of helix 8 plays a central role in invertebrates. Since the last amino acid residues of helix 8 are dispensable for rhodopsin folding and function, we propose that this domain participates in the recognition of targeting factors involved in transport to the rhabdomeres. PMID:19572012
NASA Technical Reports Server (NTRS)
Heer, E.
1973-01-01
Free-flying teleoperator systems are discussed, giving attention to earth-orbit mission considerations and Space Tug requirements, free-flying teleoperator requirements and conceptual design, system requirements for a free-flying teleoperator to despin, and the experimental evaluation of remote manipulator systems. Shuttle-Attached Manipulator Systems are considered, together with remote surface vehicle systems, manipulator systems technology, remote sensor and display technology, the man-machine interface, and control and machine intelligence. Nonspace applications are also explored, taking into account implications of nonspace applications, naval applications of remote manipulators, and hand tools and mechanical accessories for a deep submersible. Individual items are announced in this issue.
Small fruit flies sacrifice temporal acuity to maintain contrast sensitivity.
Currea, John P; Smith, Joshua L; Theobald, Jamie C
2018-06-05
Holometabolous insects, like fruit flies, grow primarily during larval development. Scarce larval feeding is common in nature and generates smaller adults. Despite the importance of vision to flies, eye size scales proportionately with body size, and smaller eyes confer poorer vision due to smaller optics. Variable larval feeding, therefore, causes within-species differences in visual processing, which have gone largely unnoticed due to ad libitum feeding in the lab that results in generally large adults. Do smaller eyes have smaller ommatidial lenses, reducing sensitivity, or broader inter-ommatidial angles, reducing acuity? And to what extent might neural processes adapt to these optical challenges with temporal and spatial summation? To understand this in the fruit fly, we generated a distribution of body lengths (1.67-2.34 mm; n = 24) and eye lengths (0.33-0.44 mm; n = 24), resembling the distribution of wild-caught flies, by removing larvae from food during their third instar. We find smaller eyes (0.19 vs.0.07 mm 2 ) have substantially fewer (978 vs. 540, n = 45) and smaller ommatidia (222 vs. 121 μm 2 ;n = 45) separated by slightly wider inter-ommatidial angles (4.5 vs.5.5°; n = 34). This corresponds to a greater loss in contrast sensitivity (<50%) than spatial acuity (<20%). Using a flight arena and psychophysics paradigm, we find that smaller flies lose little spatial acuity (0.126 vs. 0.118CPD; n = 45), and recover contrast sensitivity (2.22 for both; n = 65) by sacrificing temporal acuity (26.3 vs. 10.8Hz; n = 112) at the neural level. Therefore, smaller flies sacrifice contrast sensitivity to maintain spatial acuity optically, but recover contrast sensitivity, almost completely, by sacrificing temporal acuity neurally. Copyright © 2018. Published by Elsevier Ltd.
RIMS: An Integrated Mapping and Analysis System with Applications to Earth Sciences and Hydrology
NASA Astrophysics Data System (ADS)
Proussevitch, A. A.; Glidden, S.; Shiklomanov, A. I.; Lammers, R. B.
2011-12-01
A web-based information and computational system for analysis of spatially distributed Earth system, climate, and hydrologic data have been developed. The System allows visualization, data exploration, querying, manipulation and arbitrary calculations with any loaded gridded or vector polygon dataset. The system's acronym, RIMS, stands for its core functionality as a Rapid Integrated Mapping System. The system can be deployed for a Global scale projects as well as for regional hydrology and climatology studies. In particular, the Water Systems Analysis Group of the University of New Hampshire developed the global and regional (Northern Eurasia, pan-Arctic) versions of the system with different map projections and specific data. The system has demonstrated its potential for applications in other fields of Earth sciences and education. The key Web server/client components of the framework include (a) a visualization engine built on Open Source libraries (GDAL, PROJ.4, etc.) that are utilized in a MapServer; (b) multi-level data querying tools built on XML server-client communication protocols that allow downloading map data on-the-fly to a client web browser; and (c) data manipulation and grid cell level calculation tools that mimic desktop GIS software functionality via a web interface. Server side data management of the system is designed around a simple database of dataset metadata facilitating mounting of new data to the system and maintaining existing data in an easy manner. RIMS contains "built-in" river network data that allows for query of upstream areas on-demand which can be used for spatial data aggregation and analysis of sub-basin areas. RIMS is an ongoing effort and currently being used to serve a number of websites hosting a suite of hydrologic, environmental and other GIS data.
Mistry, Rupal; Kounatidis, Ilias; Ligoxygakis, Petros
2017-01-01
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism’s life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes that led to conflicting results. We used Drosophila as a genetically tractable system with a simpler gut bacterial population structure streamlined genetic backgrounds and established cross schemes to address this issue. We found that, depending on their genetic background, young flies had microbiota of different diversities that converged with age to the same Acetobacteraceae-dominated pattern in healthy flies. This pattern was accelerated in immune-compromised flies with higher bacterial load and gut cell death. Nevertheless, immune-compromised flies resembled their genetic background, indicating that familial transmission was the main force regulating gut microbiota. In contrast, flies with a constitutively active immune system had microbiota readily distinguishable from their genetic background with the introduction and establishment of previously undetectable bacterial families. This indicated the influence of immunity over familial transmission. Moreover, hyperactive immunity and increased enterocyte death resulted in the highest bacterial load observed starting from early adulthood. Cohousing experiments showed that the microenvironment also played an important role in the structure of the microbiota where flies with constitutive immunity defined the gut microbiota of their cohabitants. Our data show that, in Drosophila, constitutively active immunity shapes the structure and density of gut microbiota. PMID:28413160
Direct Visual and Radar Methods for the Detection, Quantification, and Prediction of Bird Migration.
1980-04-30
domesticus), 12 cm2 ; and Rock Dove ( Columba livia ), 80 cm’. Eastwood (1967) has given the radar cross sections of a number of European birds as mea...in Table 4 and Table 5 it is apparent that a single pigeon ( Columba livia ) flying toward the radar should theoretically produce an echo on the ASR-4
USDA-ARS?s Scientific Manuscript database
Field population surveillance of a targeted insect pest species is critical to the evaluation of management programs such as the sterile insect technique. Use of markers to distinguish released insects from the field population is essential, but fluorescent powder dyes used on tephritid species are ...
Evaluation of trap types and food attractants for Rhagoletis cerasi (Diptera: Tephritidae).
Katsoyannos, B I; Papadopoulos, N T; Stavridis, D
2000-06-01
Trapping experiments were conducted during the period of flight activity of the cherry fruit fly Rhagoletis cerasi L. in the area of Thessaloniki, northern Greece, during the years 1993-1997 to test several traps alone and in combination with different food attractants. Yellow sticky-coated visual traps were more effective than McPhail-type traps baited with different food attractants. Of the visual traps, the most effective was the yellow Rebell trap. The Rebell trap, provided with a dispenser containing a slow release formulation of ammonium acetate attached to the lower part of the trap, was found to be the most effective of all treatments tested, capturing approximately 50% more R. cerasi flies than the Rebell trap without any attractant. Ammonium carbonate dispensers did not increase the performance of Rebell traps. More mature females were captured in Rebell traps baited with or without ammonium acetate than in McPhail-type traps baited with ammonium acetate. This study demonstrates that Rebell traps baited with an ammonium acetate dispenser can provide a more effective tool for monitoring and mass trapping of R. cerasi than the currently used unbaited Rebell traps.
Honeybee Odometry: Performance in Varying Natural Terrain
Tautz, Juergen; Zhang, Shaowu; Spaethe, Johannes; Brockmann, Axel; Si, Aung
2004-01-01
Recent studies have shown that honeybees flying through short, narrow tunnels with visually textured walls perform waggle dances that indicate a much greater flight distance than that actually flown. These studies suggest that the bee's “odometer” is driven by the optic flow (image motion) that is experienced during flight. One might therefore expect that, when bees fly to a food source through a varying outdoor landscape, their waggle dances would depend upon the nature of the terrain experienced en route. We trained honeybees to visit feeders positioned along two routes, each 580 m long. One route was exclusively over land. The other was initially over land, then over water and, finally, again over land. Flight over water resulted in a significantly flatter slope of the waggle-duration versus distance regression, compared to flight over land. The mean visual contrast of the scenes was significantly greater over land than over water. The results reveal that, in outdoor flight, the honeybee's odometer does not run at a constant rate; rather, the rate depends upon the properties of the terrain. The bee's perception of distance flown is therefore not absolute, but scene-dependent. These findings raise important and interesting questions about how these animals navigate reliably. PMID:15252454