Development of an attention-touch control for manual cervical distraction: a pilot randomized clinical trial for patients with neck pain.

PubMed

Gudavalli, M Ram; Salsbury, Stacie A; Vining, Robert D; Long, Cynthia R; Corber, Lance; Patwardhan, Avinash G; Goertz, Christine M

2015-06-05

Manual cervical distraction (MCD) is a traction-based therapy performed with a manual contact over the cervical region producing repeating cycles while patients lie prone. This study evaluated a traction force-based minimal intervention for use as an attention-touch control in clinical trials of MCD for patients with chronic neck pain. We conducted a mixed-methods, pilot randomized clinical trial in adults with chronic neck pain. Participants were allocated to three traction force ranges of MCD: low force/minimal intervention (0-20 N), medium force (21-50 N), or high force (51-100 N). Clinicians delivered five treatments over two weeks consisting of three sets of five cycles of MCD at the C5 vertebra and occiput. Traction forces were measured at each treatment. Patient-reported outcomes included a pain visual analogue scale (VAS), Neck Disability Index (NDI), Credibility and Expectancy Questionnaire (CEQ), and adverse effects. A qualitative interview evaluated treatment group allocation perceptions. We randomized 48 participants, allocating an average of five each month. Forty-five participants completed the trial with three participants lost to follow-up. Most participants were women (65%) and white (92%) with a mean (SD) age of 46.8 (12.5) years. Mean traction force values were within the prescribed force ranges for each group at the C5 and occiput levels. Neck pain VAS demonstrated a benefit for high traction force MCD compared to the low force group [adjusted mean difference 15.6; 95% confidence interval (CI) 1.6 to 29.7]. Participants in the medium traction force group demonstrated improvements in NDI compared to the low force group (adjusted mean difference 3.0; 95% CI 0.1 to 5.9), as did participants in the high traction force group (adjusted mean difference 2.7; 95% CI -0.1 to 5.6). CEQ favored the high force group. Most low force participants correctly identified their treatment allocation in the qualitative interview. No serious adverse events were documented. This pilot study demonstrated the feasibility of a clinical trial protocol and the utility of a traction-based, minimal intervention as an attention-touch control for future efficacy trials of MCD for patients with neck pain. ClinicalTrials.gov NCT01765751 (Registration Date 30 May 2012).

A MEMS hardness sensor with reduced contact force dependence based on the reference plane concept aimed for medical applications

NASA Astrophysics Data System (ADS)

Maeda, Yusaku; Terao, Kyohei; Shimokawa, Fusao; Takao, Hidekuni

2016-04-01

In this study, the stable detection principle of a MEMS hardness sensor with “reference plane” structure is theoretically analyzed and demonstrated with experimental results. Hardness measurement independent of contact force instability is realized by the optimum design of the reference plane. The fabricated devices were evaluated, and a “shore A” hardness scale (JIS K 6301 A) was obtained as the reference in the range from A1 to A54 under a stable contact force. The contact force dependence on hardness sensor signals was effectively reduced by 96.6% using our reference plane design. Below 5 N contact force, the maximal signal error of hardness is suppressed to A8. This result corresponds to the detection capability for fat hardness, even when the contact force is unstable. Through experiments, stable detection of human body hardness has been demonstrated without any control of contact force.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, S.S.; Zhu, S.; Cai, Y.

Motion-dependent magnetic forces are the key elements in the study of magnetically levitated vehicle (maglev) system dynamics. In the past, most maglev-system designs were based on a quasisteady-motion theory of magnetic forces. This report presents an experimental and analytical study that will enhance our understanding of the role of unsteady-motion-dependent magnetic forces and demonstrate an experimental technique that can be used to measure those unsteady magnetic forces directly. The experimental technique provides a useful tool to measure motion-dependent magnetic forces for the prediction and control of maglev systems.

Monitoring developmental force distributions in reconstituted embryonic epithelia.

PubMed

Przybyla, L; Lakins, J N; Sunyer, R; Trepat, X; Weaver, V M

2016-02-01

The way cells are organized within a tissue dictates how they sense and respond to extracellular signals, as cues are received and interpreted based on expression and organization of receptors, downstream signaling proteins, and transcription factors. Part of this microenvironmental context is the result of forces acting on the cell, including forces from other cells or from the cellular substrate or basement membrane. However, measuring forces exerted on and by cells is difficult, particularly in an in vivo context, and interpreting how forces affect downstream cellular processes poses an even greater challenge. Here, we present a simple method for monitoring and analyzing forces generated from cell collectives. We demonstrate the ability to generate traction force data from human embryonic stem cells grown in large organized epithelial sheets to determine the magnitude and organization of cell-ECM and cell-cell forces within a self-renewing colony. We show that this method can be used to measure forces in a dynamic hESC system and demonstrate the ability to map intracolony protein localization to force organization. Copyright © 2015 Elsevier Inc. All rights reserved.

A predictive bone drilling force model for haptic rendering with experimental validation using fresh cadaveric bone.

PubMed

Lin, Yanping; Chen, Huajiang; Yu, Dedong; Zhang, Ying; Yuan, Wen

2017-01-01

Bone drilling simulators with virtual and haptic feedback provide a safe, cost-effective and repeatable alternative to traditional surgical training methods. To develop such a simulator, accurate haptic rendering based on a force model is required to feedback bone drilling forces based on user input. Current predictive bone drilling force models based on bovine bones with various drilling conditions and parameters are not representative of the bone drilling process in bone surgery. The objective of this study was to provide a bone drilling force model for haptic rendering based on calibration and validation experiments in fresh cadaveric bones with different bone densities. Using a commonly used drill bit geometry (2 mm diameter), feed rates (20-60 mm/min) and spindle speeds (4000-6000 rpm) in orthognathic surgeries, the bone drilling forces of specimens from two groups were measured and the calibration coefficients of the specific normal and frictional pressures were determined. The comparison of the predicted forces and the measured forces from validation experiments with a large range of feed rates and spindle speeds demonstrates that the proposed bone drilling forces can predict the trends and average forces well. The presented bone drilling force model can be used for haptic rendering in surgical simulators.

A new scheme of force reflecting control

NASA Technical Reports Server (NTRS)

Kim, Won S.

1992-01-01

A new scheme of force reflecting control has been developed that incorporates position-error-based force reflection and robot compliance control. The operator is provided with a kinesthetic force feedback which is proportional to the position error between the operator-commanded and the actual position of the robot arm. Robot compliance control, which increases the effective compliance of the robot, is implemented by low pass filtering the outputs of the force/torque sensor mounted on the base of robot hand and using these signals to alter the operator's position command. This position-error-based force reflection scheme combined with shared compliance control has been implemented successfully to the Advanced Teleoperation system consisting of dissimilar master-slave arms. Stability measurements have demonstrated unprecedentedly high force reflection gains of up to 2 or 3, even though the slave arm is much stiffer than operator's hand holding the force reflecting hand controller. Peg-in-hole experiments were performed with eight different operating modes to evaluate the new force-reflecting control scheme. Best task performance resulted with this new control scheme.

Force Field for Water Based on Neural Network.

PubMed

Wang, Hao; Yang, Weitao

2018-05-18

We developed a novel neural network based force field for water based on training with high level ab initio theory. The force field was built based on electrostatically embedded many-body expansion method truncated at binary interactions. Many-body expansion method is a common strategy to partition the total Hamiltonian of large systems into a hierarchy of few-body terms. Neural networks were trained to represent electrostatically embedded one-body and two-body interactions, which require as input only one and two water molecule calculations at the level of ab initio electronic structure method CCSD/aug-cc-pVDZ embedded in the molecular mechanics water environment, making it efficient as a general force field construction approach. Structural and dynamic properties of liquid water calculated with our force field show good agreement with experimental results. We constructed two sets of neural network based force fields: non-polarizable and polarizable force fields. Simulation results show that the non-polarizable force field using fixed TIP3P charges has already behaved well, since polarization effects and many-body effects are implicitly included due to the electrostatic embedding scheme. Our results demonstrate that the electrostatically embedded many-body expansion combined with neural network provides a promising and systematic way to build the next generation force fields at high accuracy and low computational costs, especially for large systems.

(abstract) Using TOPEX/Poseidon Sea Level Observations to Test the Sensitivity of an Ocean Model to Wind Forcing

NASA Technical Reports Server (NTRS)

Fu, Lee-Lueng; Chao, Yi

1996-01-01

It has been demonstrated that current-generation global ocean general circulation models (OGCM) are able to simulate large-scale sea level variations fairly well. In this study, a GFDL/MOM-based OGCM was used to investigate its sensitivity to different wind forcing. Simulations of global sea level using wind forcing from the ERS-1 Scatterometer and the NMC operational analysis were compared to the observations made by the TOPEX/Poseidon (T/P) radar altimeter for a two-year period. The result of the study has demonstrated the sensitivity of the OGCM to the quality of wind forcing, as well as the synergistic use of two spaceborne sensors in advancing the study of wind-driven ocean dynamics.

Spring roll dielectric elastomer actuators for a portable force feedback glove

NASA Astrophysics Data System (ADS)

Zhang, Rui; Lochmatter, Patrick; Kunz, Andreas; Kovacs, Gabor

2006-03-01

Miniature spring roll dielectric elastomer actuators for a novel kinematic-free force feedback concept were manufactured and experimentally characterized. The actuators exhibited a maximum blocking force of 7.2 N and a displacement of 5 mm. The theoretical considerations based on the material's incompressibility were discussed in order to estimate the actuator behavior under blocked-strain activation and free-strain activation. One prototype was built for the demonstration of the proposed force feedback concept.

Drastic reduction in the growth temperature of graphene on copper via enhanced London dispersion force

PubMed Central

Choi, Jin-Ho; Li, Zhancheng; Cui, Ping; Fan, Xiaodong; Zhang, Hui; Zeng, Changgan; Zhang, Zhenyu

2013-01-01

London dispersion force is ubiquitous in nature, and is increasingly recognized to be an important factor in a variety of surface processes. Here we demonstrate unambiguously the decisive role of London dispersion force in non-equilibrium growth of ordered nanostructures on metal substrates using aromatic source molecules. Our first-principles based multi-scale modeling shows that a drastic reduction in the growth temperature, from ~1000°C to ~300°C, can be achieved in graphene growth on Cu(111) when the typical carbon source of methane is replaced by benzene or p-Terphenyl. The London dispersion force enhances their adsorption energies by about (0.5–1.8) eV, thereby preventing their easy desorption, facilitating dehydrogenation, and promoting graphene growth at much lower temperatures. These quantitative predictions are validated in our experimental tests, showing convincing demonstration of monolayer graphene growth using the p-Terphenyl source. The general trends established are also more broadly applicable in molecular synthesis of surface-based nanostructures. PMID:23722566

ENHANCED REMEDIATION DEMONSTRATIONS AT HILL AFB: INTRODUCTION

EPA Science Inventory

Nine enhanced aquifer remediation technologies were demonstrated side-by-side at a Hill Air Force Base Chemical Disposal Pit/Fire Training Area site. The demonstrations were performed inside 3 x 5 m cells isolated from the surrounding shallow aquifer by steel piling. The site w...

Membrane-based actuation for high-speed single molecule force spectroscopy studies using AFM.

PubMed

Sarangapani, Krishna; Torun, Hamdi; Finkler, Ofer; Zhu, Cheng; Degertekin, Levent

2010-07-01

Atomic force microscopy (AFM)-based dynamic force spectroscopy of single molecular interactions involves characterizing unbinding/unfolding force distributions over a range of pulling speeds. Owing to their size and stiffness, AFM cantilevers are adversely affected by hydrodynamic forces, especially at pulling speeds >10 microm/s, when the viscous drag becomes comparable to the unbinding/unfolding forces. To circumvent these adverse effects, we have fabricated polymer-based membranes capable of actuating commercial AFM cantilevers at speeds >or=100 microm/s with minimal viscous drag effects. We have used FLUENT, a computational fluid dynamics (CFD) software, to simulate high-speed pulling and fast actuation of AFM cantilevers and membranes in different experimental configurations. The simulation results support the experimental findings on a variety of commercial AFM cantilevers and predict significant reduction in drag forces when membrane actuators are used. Unbinding force experiments involving human antibodies using these membranes demonstrate that it is possible to achieve bond loading rates >or=10(6) pN/s, an order of magnitude greater than that reported with commercial AFM cantilevers and systems.

NATURAL ATTENUATION OF FUEL HYDROCARBONS AT MULTIPLE AIR FORCE BASE DEMONSTRATION SITES

EPA Science Inventory

A major initiative to evaluate monitored natural attenuation(MNA) of ground water contaminated with fuel hydrocarbons began in June 1993 and continued through October 2000. During this time site characterization studies, both initial and follow-up, were conducted at 28 Air Forc...

Safe Tissue Manipulation in Retinal Microsurgery via Motorized Instruments with Force Sensing.

PubMed

Gonenc, Berk; Gehlbach, Peter; Taylor, Russell H; Iordachita, Iulian

2017-01-01

Retinal microsurgery involves careful manipulation of delicate tissues by applying very small amount of forces most of which lie below the tactile sensory threshold of the surgeons. Membrane peeling is a common task in this domain, where application of excessive peeling forces can easily lead to serious complications, hence needs to be avoided. To quantify tool-tissue interaction forces during retinal microsurgery, various force-sensing tools were developed based on fiber Bragg grating sensors, yet the most beneficial way of using the acquired force information is currently unknown. In this study, using a motorized force-sensing micro-forceps tool, we develop an assistive method that enhances safety during membrane peeling by automatically opening the forceps and releasing the tissue based on the detected peeling forces. Through peeling experiments using bandages, we demonstrate that our method can effectively maintain the peeling force at a safe level even in case of non-homogeneous adhesion properties of the membrane.

Reliability of Single-Leg Balance and Landing Tests in Rugby Union; Prospect of Using Postural Control to Monitor Fatigue

PubMed Central

Troester, Jordan C.; Jasmin, Jason G.; Duffield, Rob

2018-01-01

The present study examined the inter-trial (within test) and inter-test (between test) reliability of single-leg balance and single-leg landing measures performed on a force plate in professional rugby union players using commercially available software (SpartaMARS, Menlo Park, USA). Twenty-four players undertook test – re-test measures on two occasions (7 days apart) on the first training day of two respective pre-season weeks following 48h rest and similar weekly training loads. Two 20s single-leg balance trials were performed on a force plate with eyes closed. Three single-leg landing trials were performed by jumping off two feet and landing on one foot in the middle of a force plate 1m from the starting position. Single-leg balance results demonstrated acceptable inter-trial reliability (ICC = 0.60-0.81, CV = 11-13%) for sway velocity, anterior-posterior sway velocity, and mediolateral sway velocity variables. Acceptable inter-test reliability (ICC = 0.61-0.89, CV = 7-13%) was evident for all variables except mediolateral sway velocity on the dominant leg (ICC = 0.41, CV = 15%). Single-leg landing results only demonstrated acceptable inter-trial reliability for force based measures of relative peak landing force and impulse (ICC = 0.54-0.72, CV = 9-15%). Inter-test results indicate improved reliability through the averaging of three trials with force based measures again demonstrating acceptable reliability (ICC = 0.58-0.71, CV = 7-14%). Of the variables investigated here, total sway velocity and relative landing impulse are the most reliable measures of single-leg balance and landing performance, respectively. These measures should be considered for monitoring potential changes in postural control in professional rugby union. Key points Single-leg balance demonstrated acceptable inter-trial and inter-test reliability. Single-leg landing demonstrated good inter-trial and inter-test reliability for measures of relative peak landing force and relative impulse, but not time to stabilization. Of the variables investigated, sway velocity and relative landing impulse are the most reliable measures of single-leg balance and landing respectively, and should considered for monitoring changes in postural control. PMID:29769817

Experimental Demonstration of a Synthetic Lorentz Force by Using Radiation Pressure.

PubMed

Šantić, N; Dubček, T; Aumiler, D; Buljan, H; Ban, T

2015-09-02

Synthetic magnetism in cold atomic gases opened the doors to many exciting novel physical systems and phenomena. Ubiquitous are the methods used for the creation of synthetic magnetic fields. They include rapidly rotating Bose-Einstein condensates employing the analogy between the Coriolis and the Lorentz force, and laser-atom interactions employing the analogy between the Berry phase and the Aharonov-Bohm phase. Interestingly, radiation pressure - being one of the most common forces induced by light - has not yet been used for synthetic magnetism. We experimentally demonstrate a synthetic Lorentz force, based on the radiation pressure and the Doppler effect, by observing the centre-of-mass motion of a cold atomic cloud. The force is perpendicular to the velocity of the cold atomic cloud, and zero for the cloud at rest. Our novel concept is straightforward to implement in a large volume, for a broad range of velocities, and can be extended to different geometries.

ESTCP Munitions Response Live Site Demonstrations Southwest Proving Ground, Arkansas Andersen Air Force Base, Guam Cost and Performance Report

DTIC Science & Technology

2017-09-01

CANNOT BE ANALYZED ....... 17  7.0  COST BENEFIT ANALYSIS...20  7.3  COST BENEFIT ...sites and assess the performance and cost benefits of implementing AGC technologies. OBJECTIVES OF THE DEMONSTRATION The demonstrations were

Cavity magnomechanics

PubMed Central

Zhang, Xufeng; Zou, Chang-Ling; Jiang, Liang; Tang, Hong X.

2016-01-01

A dielectric body couples with electromagnetic fields through radiation pressure and electrostrictive forces, which mediate phonon-photon coupling in cavity optomechanics. In a magnetic medium, according to the Korteweg-Helmholtz formula, which describes the electromagnetic force density acting on a medium, magneostrictive forces should arise and lead to phonon-magnon interaction. We report such a coupled phonon-magnon system based on ferrimagnetic spheres, which we term as cavity magnomechanics, by analogy to cavity optomechanics. Coherent phonon-magnon interactions, including electromagnetically induced transparency and absorption, are demonstrated. Because of the strong hybridization of magnon and microwave photon modes and their high tunability, our platform exhibits new features including parametric amplification of magnons and phonons, triple-resonant photon-magnon-phonon coupling, and phonon lasing. Our work demonstrates the fundamental principle of cavity magnomechanics and its application as a new information transduction platform based on coherent coupling between photons, phonons, and magnons. PMID:27034983

Hierarchical atom type definitions and extensible all-atom force fields.

PubMed

Jin, Zhao; Yang, Chunwei; Cao, Fenglei; Li, Feng; Jing, Zhifeng; Chen, Long; Shen, Zhe; Xin, Liang; Tong, Sijia; Sun, Huai

2016-03-15

The extensibility of force field is a key to solve the missing parameter problem commonly found in force field applications. The extensibility of conventional force fields is traditionally managed in the parameterization procedure, which becomes impractical as the coverage of the force field increases above a threshold. A hierarchical atom-type definition (HAD) scheme is proposed to make extensible atom type definitions, which ensures that the force field developed based on the definitions are extensible. To demonstrate how HAD works and to prepare a foundation for future developments, two general force fields based on AMBER and DFF functional forms are parameterized for common organic molecules. The force field parameters are derived from the same set of quantum mechanical data and experimental liquid data using an automated parameterization tool, and validated by calculating molecular and liquid properties. The hydration free energies are calculated successfully by introducing a polarization scaling factor to the dispersion term between the solvent and solute molecules. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.

Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips.

PubMed

Deng, Yongbo; Fan, Jianhua; Zhou, Song; Zhou, Teng; Wu, Junfeng; Li, Yin; Liu, Zhenyu; Xuan, Ming; Wu, Yihui

2014-03-01

Based on the Euler force induced by the acceleration of compact disk (CD)-like microfluidic chip, this paper presents a novel actuation mechanism for siphon valving. At the preliminary stage of acceleration, the Euler force in the tangential direction of CD-like chip takes the primary place compared with the centrifugal force to function as the actuation of the flow, which fills the siphon and actuates the siphon valving. The Euler force actuation mechanism is demonstrated by the numerical solution of the phase-field based mathematical model for the flow in siphon valve. In addition, experimental validation is implemented in the polymethylmethacrylate-based CD-like microfluidic chip manufactured using CO2 laser engraving technique. To prove the application of the proposed Euler force actuation mechanism, whole blood separation and plasma extraction has been conducted using the Euler force actuated siphon valving. The newly introduced actuation mechanism overcomes the dependence on hydrophilic capillary filling of siphon by avoiding external manipulation or surface treatments of polymeric material. The sacrifice for highly integrated processing in pneumatic pumping technique is also prevented by excluding the volume-occupied compressed air chamber.

Dynamical and fractal properties in periodically forced stretch-twist-fold (STF) flow

NASA Astrophysics Data System (ADS)

Aqeel, Muhammad; Ahmad, Salman; Azam, Anam; Ahmed, Faizan

2017-05-01

The periodically forced stretch-twist-fold (STF) flow is introduced in this article. The nonlinear behavior of the STF flow with periodic force along the y -axis is investigated analytically and numerically. The STF flow is a prototype of the dynamo theory that proposes a mechanism of magnetic field generation continuously. The stability analysis is done by Routh Huwritz criteria and Cardano method. Chasing chaos through numerical simulation is determined to demonstrate the chaotic behavior of the forced STF flow. With the help of fractal processes based on the forced STF flow, a multi-wing forced STF flow is obtained that gives a n -wing forced STF flow system.

77 FR 46439 - Medicare Program; Prior Authorization for Power Mobility Device (PMD) Demonstration

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-03

... DOJ. Medicare Fraud Strike Force teams are a key component of HEAT, since their inception and based on... primary focus of investigation for these strike forces. The Comprehensive Error Rate Testing (CERT... various prior authorization scenarios: Scenario 1: When a submitter sends a prior authorization request to...

Topographical and Chemical Imaging of a Phase Separated Polymer Using a Combined Atomic Force Microscopy/Infrared Spectroscopy/Mass Spectrometry Platform

DOE PAGES

Tai, Tamin; Karácsony, Orsolya; Bocharova, Vera; ...

2016-02-18

This article describes how the use of a hybrid atomic force microscopy/infrared spectroscopy/mass spectrometry imaging platform was demonstrated for the acquisition and correlation of nanoscale sample surface topography and chemical images based on infrared spectroscopy and mass spectrometry.

MEMS resonant load cells for micro-mechanical test frames: feasibility study and optimal design

NASA Astrophysics Data System (ADS)

Torrents, A.; Azgin, K.; Godfrey, S. W.; Topalli, E. S.; Akin, T.; Valdevit, L.

2010-12-01

This paper presents the design, optimization and manufacturing of a novel micro-fabricated load cell based on a double-ended tuning fork. The device geometry and operating voltages are optimized for maximum force resolution and range, subject to a number of manufacturing and electromechanical constraints. All optimizations are enabled by analytical modeling (verified by selected finite elements analyses) coupled with an efficient C++ code based on the particle swarm optimization algorithm. This assessment indicates that force resolutions of ~0.5-10 nN are feasible in vacuum (~1-50 mTorr), with force ranges as large as 1 N. Importantly, the optimal design for vacuum operation is independent of the desired range, ensuring versatility. Experimental verifications on a sub-optimal device fabricated using silicon-on-glass technology demonstrate a resolution of ~23 nN at a vacuum level of ~50 mTorr. The device demonstrated in this article will be integrated in a hybrid micro-mechanical test frame for unprecedented combinations of force resolution and range, displacement resolution and range, optical (or SEM) access to the sample, versatility and cost.

Detection of cancerous cervical cells using physical adhesion of fluorescent silica particles and centripetal force

PubMed Central

Gaikwad, Ravi M.; Dokukin, Maxim E.; Iyer, K. Swaminathan; Woodworth, Craig D.; Volkov, Dmytro O.; Sokolov, Igor

2012-01-01

Here we describe a non-traditional method to identify cancerous human cervical epithelial cells in a culture dish based on physical interaction between silica beads and cells. It is a simple optical fluorescence-based technique which detects the relative difference in the amount of fluorescent silica beads physically adherent to surfaces of cancerous and normal cervical cells. The method utilizes the centripetal force gradient that occurs in a rotating culture dish. Due to the variation in the balance between adhesion and centripetal forces, cancerous and normal cells demonstrate clearly distinctive distributions of the fluorescent particles adherent to the cell surface over the culture dish. The method demonstrates higher adhesion of silica particles to normal cells compared to cancerous cells. The difference in adhesion was initially observed by atomic force microscopy (AFM). The AFM data were used to design the parameters of the rotational dish experiment. The optical method that we describe is much faster and technically simpler than AFM. This work provides proof of the concept that physical interactions can be used to accurately discriminate normal and cancer cells. PMID:21305062

Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

PubMed Central

Kulik, Andrzej J; Lee, Kyumin; Pyka-Fościak, Grazyna; Nowak, Wieslaw

2015-01-01

Summary The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin–carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces strongly dominate over the non-specific ones. However, obtaining quantitative results requires the complex and tedious calibration of a torsional force. Here, a new and relatively simple method for the calibration of the torsional force is presented. The proposed calibration method is validated through the measurement of the interaction forces between human fibronectin and its monoclonal antibody. The results obtained using LFM and AFM-based classical force spectroscopies showed similar unbinding forces recorded at similar loading rates. Our studies verify that the proposed lateral force calibration method can be applied to study single molecule interactions. PMID:26114080

Panel Flutter Emulation Using a Few Concentrated Forces

NASA Astrophysics Data System (ADS)

Dhital, Kailash; Han, Jae-Hung

2018-04-01

The objective of this paper is to study the feasibility of panel flutter emulation using a few concentrated forces. The concentrated forces are considered to be equivalent to aerodynamic forces. The equivalence is carried out using surface spline method and principle of virtual work. The structural modeling of the plate is based on the classical plate theory and the aerodynamic modeling is based on the piston theory. The present approach differs from the linear panel flutter analysis in scheming the modal aerodynamics forces with unchanged structural properties. The solutions for the flutter problem are obtained numerically using the standard eigenvalue procedure. A few concentrated forces were considered with an optimization effort to decide their optimal locations. The optimization process is based on minimizing the error between the flutter bounds from emulated and linear flutter analysis method. The emulated flutter results for the square plate of four different boundary conditions using six concentrated forces are obtained with minimal error to the reference value. The results demonstrated the workability and viability of using concentrated forces in emulating real panel flutter. In addition, the paper includes the parametric studies of linear panel flutter whose proper literatures are not available.

Magnetoelectric force microscopy based on magnetic force microscopy with modulated electric field.

PubMed

Geng, Yanan; Wu, Weida

2014-05-01

We present the realization of a mesoscopic imaging technique, namely, the Magnetoelectric Force Microscopy (MeFM), for visualization of local magnetoelectric effect. The basic principle of MeFM is the lock-in detection of local magnetoelectric response, i.e., the electric field-induced magnetization, using magnetic force microscopy. We demonstrate MeFM capability by visualizing magnetoelectric domains on single crystals of multiferroic hexagonal manganites. Results of several control experiments exclude artifacts or extrinsic origins of the MeFM signal. The parameters are tuned to optimize the signal to noise ratio.

Connecting the Force from Space: The IRIS Joint Capability Technology Demonstration

DTIC Science & Technology

2010-01-01

the Joint in Joint Capability Technology Demonstration, we have two sponsors, both U.S. Strategic Command and the Defense Information Systems...Capability Technology Demonstration will provide an excellent source of data on space-based Internet Protocol net- working. Operational... Internet Routing in Space Joint Capability Technology Demonstration Operational Manager, Space and Missile Defense Battle Lab, Colorado Springs

A force-based protein biochip

NASA Astrophysics Data System (ADS)

Blank, K.; Mai, T.; Gilbert, I.; Schiffmann, S.; Rankl, J.; Zivin, R.; Tackney, C.; Nicolaus, T.; Spinnler, K.; Oesterhelt, F.; Benoit, M.; Clausen-Schaumann, H.; Gaub, H. E.

2003-09-01

A parallel assay for the quantification of single-molecule binding forces was developed based on differential unbinding force measurements where ligand-receptor interactions are compared with the unzipping forces of DNA hybrids. Using the DNA zippers as molecular force sensors, the efficient discrimination between specific and nonspecific interactions was demonstrated for small molecules binding to specific receptors, as well as for protein-protein interactions on protein arrays. Finally, an antibody sandwich assay with different capture antibodies on one chip surface and with the detection antibodies linked to a congruent surface via the DNA zippers was used to capture and quantify a recombinant hepatitis C antigen from solution. In this case, the DNA zippers enable not only discrimination between specific and nonspecific binding, but also allow for the local application of detection antibodies, thereby eliminating false-positive results caused by cross-reactive antibodies and nonspecific binding.

X-31 wing removal

NASA Image and Video Library

1995-05-18

U.S. and German personnel of the X-31 Enhanced Fighter Maneuverability Technology Demonstrator aircraft program removing the right wing of the aircraft, which was ferried from Edwards Air Force Base, California, to Europe on May 22, 1995 aboard an Air Force Reserve C-5 transport. The X-31, based at the NASA Dryden Flight Research Center was ferried to Europe and flown in the Paris Air Show in June. The wing of the X-31 was removed on May 18, 1995, to allow the aircraft to fit inside the C-5 fuselage. Officials of the X-31 project used Manching, Germany, as a staging base to prepare the aircraft for the flight demonstration. At the air show, the X-31 demonstrated the value of using thrust vectoring (directing engine exhaust flow) coupled with advanced flight control systems to provide controlled flight at very high angles of attack. The aircraft arrived back at Edwards in a Air Force Reserve C-5 on June 25, 1995 and off loaded at Dryden June 27. The X-31 aircraft was developed jointly by Rockwell International's North American Aircraft Division (now part of Boeing) and Daimler-Benz Aerospace (formerly Messerschmitt-Bolkow-Blohm), under sponsorship by the U.S. Department of Defense and the German Federal Ministry of Defense.

E-cadherin-mediated force transduction signals regulate global cell mechanics

PubMed Central

Muhamed, Ismaeel; Wu, Jun; Sehgal, Poonam; Kong, Xinyu; Tajik, Arash; Wang, Ning

2016-01-01

ABSTRACT This report elucidates an E-cadherin-based force-transduction pathway that triggers changes in cell mechanics through a mechanism requiring epidermal growth factor receptor (EGFR), phosphoinositide 3-kinase (PI3K), and the downstream formation of new integrin adhesions. This mechanism operates in addition to local cytoskeletal remodeling triggered by conformational changes in the E-cadherin-associated protein α-catenin, at sites of mechanical perturbation. Studies using magnetic twisting cytometry (MTC), together with traction force microscopy (TFM) and confocal imaging identified force-activated E-cadherin-specific signals that integrate cadherin force transduction, integrin activation and cell contractility. EGFR is required for the downstream activation of PI3K and myosin-II-dependent cell stiffening. Our findings also demonstrated that α-catenin-dependent cytoskeletal remodeling at perturbed E-cadherin adhesions does not require cell stiffening. These results broaden the repertoire of E-cadherin-based force transduction mechanisms, and define the force-sensitive signaling network underlying the mechano-chemical integration of spatially segregated adhesion receptors. PMID:26966187

Direct current force sensing device based on compressive spring, permanent magnet, and coil-wound magnetostrictive/piezoelectric laminate.

PubMed

Leung, Chung Ming; Or, Siu Wing; Ho, S L

2013-12-01

A force sensing device capable of sensing dc (or static) compressive forces is developed based on a NAS106N stainless steel compressive spring, a sintered NdFeB permanent magnet, and a coil-wound Tb(0.3)Dy(0.7)Fe(1.92)/Pb(Zr, Ti)O3 magnetostrictive∕piezoelectric laminate. The dc compressive force sensing in the device is evaluated theoretically and experimentally and is found to originate from a unique force-induced, position-dependent, current-driven dc magnetoelectric effect. The sensitivity of the device can be increased by increasing the spring constant of the compressive spring, the size of the permanent magnet, and/or the driving current for the coil-wound laminate. Devices of low-force (20 N) and high-force (200 N) types, showing high output voltages of 262 and 128 mV peak, respectively, are demonstrated at a low driving current of 100 mA peak by using different combinations of compressive spring and permanent magnet.

A Sub-Millimetric 3-DOF Force Sensing Instrument with Integrated Fiber Bragg Grating for Retinal Microsurgery

PubMed Central

He, Xingchi; Handa, James; Gehlbach, Peter; Taylor, Russell; Iordachita, Iulian

2013-01-01

Vitreoretinal surgery requires very fine motor control to perform precise manipulation of the delicate tissue in the interior of the eye. Besides physiological hand tremor, fatigue, poor kinesthetic feedback, and patient movement, the absence of force sensing is one of the main technical challenges. Previous two degrees of freedom (DOF) force sensing instruments have demonstrated robust force measuring performance. The main design challenge is to incorporate high sensitivity axial force sensing. This paper reports the development of a sub-millimetric 3-DOF force sensing pick instrument based on fiber Bragg grating (FBG) sensors. The configuration of the four FBG sensors is arranged to maximize the decoupling between axial and transverse force sensing. A super-elastic nitinol flexure is designed to achieve high axial force sensitivity. An automated calibration system was developed for repeatability testing, calibration, and validation. Experimental results demonstrate a FBG sensor repeatability of 1.3 pm. The linear model for calculating the transverse forces provides an accurate global estimate. While the linear model for axial force is only locally accurate within a conical region with a 30° vertex angle, a second-order polynomial model can provide a useful global estimate for axial force. Combining the linear model for transverse forces and nonlinear model for axial force, the 3-DOF force sensing instrument can provide sub-millinewton resolution for axial force and a quarter millinewton for transverse forces. Validation with random samples show the force sensor can provide consistent and accurate measurement of three dimensional forces. PMID:24108455

Reliability of Single-Leg Balance and Landing Tests in Rugby Union; Prospect of Using Postural Control to Monitor Fatigue.

PubMed

Troester, Jordan C; Jasmin, Jason G; Duffield, Rob

2018-06-01

The present study examined the inter-trial (within test) and inter-test (between test) reliability of single-leg balance and single-leg landing measures performed on a force plate in professional rugby union players using commercially available software (SpartaMARS, Menlo Park, USA). Twenty-four players undertook test - re-test measures on two occasions (7 days apart) on the first training day of two respective pre-season weeks following 48h rest and similar weekly training loads. Two 20s single-leg balance trials were performed on a force plate with eyes closed. Three single-leg landing trials were performed by jumping off two feet and landing on one foot in the middle of a force plate 1m from the starting position. Single-leg balance results demonstrated acceptable inter-trial reliability (ICC = 0.60-0.81, CV = 11-13%) for sway velocity, anterior-posterior sway velocity, and mediolateral sway velocity variables. Acceptable inter-test reliability (ICC = 0.61-0.89, CV = 7-13%) was evident for all variables except mediolateral sway velocity on the dominant leg (ICC = 0.41, CV = 15%). Single-leg landing results only demonstrated acceptable inter-trial reliability for force based measures of relative peak landing force and impulse (ICC = 0.54-0.72, CV = 9-15%). Inter-test results indicate improved reliability through the averaging of three trials with force based measures again demonstrating acceptable reliability (ICC = 0.58-0.71, CV = 7-14%). Of the variables investigated here, total sway velocity and relative landing impulse are the most reliable measures of single-leg balance and landing performance, respectively. These measures should be considered for monitoring potential changes in postural control in professional rugby union.

TEACHING PHYSICS: A computer-based revitalization of Atwood's machine

NASA Astrophysics Data System (ADS)

Trumper, Ricardo; Gelbman, Moshe

2000-09-01

Atwood's machine is used in a microcomputer-based experiment to demonstrate Newton's second law with considerable precision. The friction force on the masses and the moment of inertia of the pulley can also be estimated.

Friction enhancement via micro-patterned wet elastomer adhesives on small intestinal surfaces.

PubMed

Kwon, Jiwoon; Cheung, Eugene; Park, Sukho; Sitti, Metin

2006-12-01

A micro-pillar-based silicone rubber adhesive coated with a thin silicone oil layer is investigated in this paper for developing friction-based clamping mechanisms for robotic endoscopic microcapsules. These adhesives are shown to enhance the frictional force between the capsule and the intestinal wall by a factor of about seven over a non-patterned flat elastomer material. In this study, tests performed on fresh samples of pig small intestine are used to optimize the diameter of the micro-pillars to maximize the frictional forces. In addition, the effects of other factors such as the oil viscosity and applied normal forces are investigated. It is demonstrated that the proposed micro-pillar pattern based elastomer adhesive exhibits a maximal frictional force when the pillar diameter is 140 microm and coated silicon oil has a very high viscosity (10,000 cSt). It is also found that the frictional force of the micro-patterned adhesive increases nonlinearly in proportion to the applied normal force. These adhesives would be used as a robust attachment material for developing robotic capsule endoscopes inside intestines with clamping capability.

Friction enhancement via micro-patterned wet elastomer adhesives on small intestinal surfaces

NASA Astrophysics Data System (ADS)

Kwon, Jiwoon; Cheung, Eugene; Park, Sukho; Sitti, Metin

2006-12-01

A micro-pillar-based silicone rubber adhesive coated with a thin silicone oil layer is investigated in this paper for developing friction-based clamping mechanisms for robotic endoscopic microcapsules. These adhesives are shown to enhance the frictional force between the capsule and the intestinal wall by a factor of about seven over a non-patterned flat elastomer material. In this study, tests performed on fresh samples of pig small intestine are used to optimize the diameter of the micro-pillars to maximize the frictional forces. In addition, the effects of other factors such as the oil viscosity and applied normal forces are investigated. It is demonstrated that the proposed micro-pillar pattern based elastomer adhesive exhibits a maximal frictional force when the pillar diameter is 140 µm and coated silicon oil has a very high viscosity (10 000 cSt). It is also found that the frictional force of the micro-patterned adhesive increases nonlinearly in proportion to the applied normal force. These adhesives would be used as a robust attachment material for developing robotic capsule endoscopes inside intestines with clamping capability.

High throughput system for magnetic manipulation of cells, polymers, and biomaterials

PubMed Central

Spero, Richard Chasen; Vicci, Leandra; Cribb, Jeremy; Bober, David; Swaminathan, Vinay; O’Brien, E. Timothy; Rogers, Stephen L.; Superfine, R.

2008-01-01

In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles ∼0.1–10 μm) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in excess of 1 nN, predicted force saturation as a function of pole material, and powerlaw dependence of F∼r−2.7±0.1. We employ this system to measure the stiffness of SR2+ Drosophila cells. MHTS technology is a key step toward a high throughput screening system for micro- and nanoscale biophysical experiments. PMID:19044357

Non-muscle myosin IIB is critical for nuclear translocation during 3D invasion

PubMed Central

Yenepalli, Aishwarya; Denais, Celine Marie; Rape, Andrew; Beach, Jordan R.; Wang, Yu-li; Schiemann, William P.; Baskaran, Harihara; Lammerding, Jan

2015-01-01

Non-muscle myosin II (NMII) is reported to play multiple roles during cell migration and invasion. However, the exact biophysical roles of different NMII isoforms during these processes remain poorly understood. We analyzed the contributions of NMIIA and NMIIB in three-dimensional (3D) migration and in generating the forces required for efficient invasion by mammary gland carcinoma cells. Using traction force microscopy and microfluidic invasion devices, we demonstrated that NMIIA is critical for generating force during active protrusion, and NMIIB plays a major role in applying force on the nucleus to facilitate nuclear translocation through tight spaces. We further demonstrate that the nuclear membrane protein nesprin-2 is a possible linker coupling NMIIB-based force generation to nuclear translocation. Together, these data reveal a central biophysical role for NMIIB in nuclear translocation during 3D invasive migration, a result with relevance not only to cancer metastasis but for 3D migration in other settings such as embryonic cell migration and wound healing. PMID:26261182

Euler force actuation mechanism for siphon valving in compact disk-like microfluidic chips

PubMed Central

Deng, Yongbo; Fan, Jianhua; Zhou, Song; Zhou, Teng; Wu, Junfeng; Li, Yin; Liu, Zhenyu; Xuan, Ming; Wu, Yihui

2014-01-01

Based on the Euler force induced by the acceleration of compact disk (CD)-like microfluidic chip, this paper presents a novel actuation mechanism for siphon valving. At the preliminary stage of acceleration, the Euler force in the tangential direction of CD-like chip takes the primary place compared with the centrifugal force to function as the actuation of the flow, which fills the siphon and actuates the siphon valving. The Euler force actuation mechanism is demonstrated by the numerical solution of the phase-field based mathematical model for the flow in siphon valve. In addition, experimental validation is implemented in the polymethylmethacrylate-based CD-like microfluidic chip manufactured using CO2 laser engraving technique. To prove the application of the proposed Euler force actuation mechanism, whole blood separation and plasma extraction has been conducted using the Euler force actuated siphon valving. The newly introduced actuation mechanism overcomes the dependence on hydrophilic capillary filling of siphon by avoiding external manipulation or surface treatments of polymeric material. The sacrifice for highly integrated processing in pneumatic pumping technique is also prevented by excluding the volume-occupied compressed air chamber. PMID:24753736

A Comparison of Paper-Based and Web-Based Testing.

ERIC Educational Resources Information Center

Cole, Rebecca Pollard; MacIsaac, Dan; Cole, David M.

The purpose of this study (1,313 college student participants) was to examine the differences in paper-based and Web-based administrations of a commonly used assessment instrument, the Force Concept Inventory (FCI) (D. Hestenes, M. Wells, and G. Swackhamer, 1992). Results demonstrated no appreciable difference on FCI scores or FCI items based on…

Highly sensitive force sensor based on balloon-like interferometer

NASA Astrophysics Data System (ADS)

Wu, Yue; Xiao, Shiying; Xu, Yao; Shen, Ya; Jiang, Youchao; Jin, Wenxing; Yang, Yuguang; Jian, Shuisheng

2018-07-01

An all-fiber highly sensitive force sensor based on modal interferometer has been presented and demonstrated. The single-mode fiber (SMF) with coating stripped is designed into a balloon-like shape to form a modal interferometer. Due to the bent SMF, the interference occurs between the core mode and cladding modes. With variation of the force applied to the balloon-like interferometer, the bending diameter changes, which caused the wavelength shift of the modal interference. Thus the measurement of the force variation can be achieved by monitoring the wavelength shift. The performances of the interferometer with different bending diameter are experimentally investigated, and the maximum force sensitivity of 24.9 pm/ μ N can be achieved with the bending diameter 14 mm ranging from 0 μ N to 1464.12 μ N. Furthermore, the proposed fiber sensor exhibits the advantages of easy fabrication and low cost, making it a suitable candidate in the optical fiber sensing field.

Phase 1 results from the Stirling-powered vehicle project

NASA Technical Reports Server (NTRS)

Shaltens, Richard K.

1988-01-01

The NASA Technology Utilization (TU) Office is sponsoring a multiyear, multiphase demonstration program to assess the technology developed under the DOE/NASA automotive Stirling engine (ASE) program with engines installed in various Air Force vehicles while being evaluated by independent third parties under realistic conditions. This paper reviews the operational history of Phase 1 with a Mod 1 Stirling engine installed in an Air Force multistop van in a variety of missions. Ten months of operation were with Air Force personnel at Langley Air Force Base, Virginia, where over 1100 hr and 4000 mi were logged on the Langley flight line. The Stirling-powered van operated on unleaded gasoline, JP-4 aircraft fuel, and diesel fuel at Langley Air Force Base. Two months of operation were completed with Deere and Company personnel in the Moline, Illinois area where over 175 hr and 2650 mi were logged on a Deere mail delivery route.

Quartz tuning fork-based frequency modulation atomic force spectroscopy and microscopy with all digital phase-locked loop

NASA Astrophysics Data System (ADS)

An, Sangmin; Hong, Mun-heon; Kim, Jongwoo; Kwon, Soyoung; Lee, Kunyoung; Lee, Manhee; Jhe, Wonho

2012-11-01

We present a platform for the quartz tuning fork (QTF)-based, frequency modulation atomic force microscopy (FM-AFM) system for quantitative study of the mechanical or topographical properties of nanoscale materials, such as the nano-sized water bridge formed between the quartz tip (˜100 nm curvature) and the mica substrate. A thermally stable, all digital phase-locked loop is used to detect the small frequency shift of the QTF signal resulting from the nanomaterial-mediated interactions. The proposed and demonstrated novel FM-AFM technique provides high experimental sensitivity in the measurement of the viscoelastic forces associated with the confined nano-water meniscus, short response time, and insensitivity to amplitude noise, which are essential for precision dynamic force spectroscopy and microscopy.

Quartz tuning fork-based frequency modulation atomic force spectroscopy and microscopy with all digital phase-locked loop.

PubMed

An, Sangmin; Hong, Mun-heon; Kim, Jongwoo; Kwon, Soyoung; Lee, Kunyoung; Lee, Manhee; Jhe, Wonho

2012-11-01

We present a platform for the quartz tuning fork (QTF)-based, frequency modulation atomic force microscopy (FM-AFM) system for quantitative study of the mechanical or topographical properties of nanoscale materials, such as the nano-sized water bridge formed between the quartz tip (~100 nm curvature) and the mica substrate. A thermally stable, all digital phase-locked loop is used to detect the small frequency shift of the QTF signal resulting from the nanomaterial-mediated interactions. The proposed and demonstrated novel FM-AFM technique provides high experimental sensitivity in the measurement of the viscoelastic forces associated with the confined nano-water meniscus, short response time, and insensitivity to amplitude noise, which are essential for precision dynamic force spectroscopy and microscopy.

A flexible piezoelectric force sensor based on PVDF fabrics

NASA Astrophysics Data System (ADS)

Wang, Y. R.; Zheng, J. M.; Ren, G. Y.; Zhang, P. H.; Xu, C.

2011-04-01

Polyvinylidene fluoride (PVDF) film has been widely investigated as a sensor and transducer material due to its high piezo-, pyro- and ferroelectric properties. To activate these properties, PVDF films require a mechanical treatment, stretching or poling. In this paper, we report on a force sensor based on PVDF fabrics with excellent flexibility and breathability, to be used as a specific human-related sensor. PVDF nanofibrous fabrics were prepared by using an electrospinning unit and characterized by means of scanning electron microscopy (SEM), FTIR spectroscopy and x-ray diffraction. Preliminary force sensors have been fabricated and demonstrated excellent sensitivity and response to external mechanical forces. This implies that promising applications can be made for sensing garment pressure, blood pressure, heartbeat rate, respiration rate and accidental impact on the human body.

A microprocessor-based table lookup approach for magnetic bearing linearization

NASA Technical Reports Server (NTRS)

Groom, N. J.; Miller, J. B.

1981-01-01

An approach for producing a linear transfer characteristic between force command and force output of a magnetic bearing actuator without flux biasing is presented. The approach is microprocessor based and uses a table lookup to generate drive signals for the magnetic bearing power driver. An experimental test setup used to demonstrate the feasibility of the approach is described, and test results are presented. The test setup contains bearing elements similar to those used in a laboratory model annular momentum control device.

Friction Compensation for Enhancing Transparency of a Teleoperator with Compliant Transmission

PubMed Central

Mahvash, Mohsen; Okamura, Allison

2009-01-01

This article presents a model-based compensator for canceling friction in the tendon-driven joints of a haptic-feedback teleoperator. Unlike position-tracking systems, a teleoperator involves an unknown environment force that prevents the use of tracking position error as a feedback to the compensator. Thus, we use a model-based feedforward friction compensator to cancel the friction forces. We provide conditions for selecting compensator parameters to ensure passivity of the teleoperator and demonstrate performance experimentally. PMID:20514151

Plasmonic Spherical Heterodimers: Reversal of Optical Binding Force Based on the Forced Breaking of Symmetry.

PubMed

Mahdy, M R C; Danesh, Md; Zhang, Tianhang; Ding, Weiqiang; Rivy, Hamim Mahmud; Chowdhury, Ariful Bari; Mehmood, M Q

2018-02-16

The stimulating connection between the reversal of near-field plasmonic binding force and the role of symmetry-breaking has not been investigated comprehensively in the literature. In this work, the symmetry of spherical plasmonic heterodimer-setup is broken forcefully by shining the light from a specific side of the set-up instead of impinging it from the top. We demonstrate that for the forced symmetry-broken spherical heterodimer-configurations: reversal of lateral and longitudinal near-field binding force follow completely distinct mechanisms. Interestingly, the reversal of longitudinal binding force can be easily controlled either by changing the direction of light propagation or by varying their relative orientation. This simple process of controlling binding force may open a novel generic way of optical manipulation even with the heterodimers of other shapes. Though it is commonly believed that the reversal of near-field plasmonic binding force should naturally occur for the presence of bonding and anti-bonding modes or at least for the Fano resonance (and plasmonic forces mostly arise from the surface force), our study based on Lorentz-force dynamics suggests notably opposite proposals for the aforementioned cases. Observations in this article can be very useful for improved sensors, particle clustering and aggregation.

Estimation of longitudinal force, lateral vehicle speed and yaw rate for four-wheel independent driven electric vehicles

NASA Astrophysics Data System (ADS)

Chen, Te; Xu, Xing; Chen, Long; Jiang, Haobing; Cai, Yingfeng; Li, Yong

2018-02-01

Accurate estimation of longitudinal force, lateral vehicle speed and yaw rate is of great significance to torque allocation and stability control for four-wheel independent driven electric vehicle (4WID-EVs). A fusion method is proposed to estimate the longitudinal force, lateral vehicle speed and yaw rate for 4WID-EVs. The electric driving wheel model (EDWM) is introduced into the longitudinal force estimation, the longitudinal force observer (LFO) is designed firstly based on the adaptive high-order sliding mode observer (HSMO), and the convergence of LFO is analyzed and proved. Based on the estimated longitudinal force, an estimation strategy is then presented in which the strong tracking filter (STF) is used to estimate lateral vehicle speed and yaw rate simultaneously. Finally, co-simulation via Carsim and Matlab/Simulink is carried out to demonstrate the effectiveness of the proposed method. The performance of LFO in practice is verified by the experiment on chassis dynamometer bench.

Field Demonstration of a Centrifugal Ultra High Pressure (UHP) P-19

DTIC Science & Technology

2010-03-01

States Air Force  Tyndall Air Force Base, FL 32403-5323 DISTRIBUTION A: Approved for public release; distribution unlimited. NOTICE AND SIGNATURE...PUBLICATION IN ACCORDANCE WITH ASSIGNED DISTRIBUTION STATEMENT. ___//SIGNATURE//______________________ ___//SIGNATURE//______________________ R...PROGRAM ELEMENT NUMBER 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 6. AUTHOR(S) 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8

Objective-lens-free Fiber-based Position Detection with Nanometer Resolution in a Fiber Optical Trapping System.

PubMed

Ti, Chaoyang; Ho-Thanh, Minh-Tri; Wen, Qi; Liu, Yuxiang

2017-10-13

Position detection with high accuracy is crucial for force calibration of optical trapping systems. Most existing position detection methods require high-numerical-aperture objective lenses, which are bulky, expensive, and difficult to miniaturize. Here, we report an affordable objective-lens-free, fiber-based position detection scheme with 2 nm spatial resolution and 150 MHz bandwidth. This fiber based detection mechanism enables simultaneous trapping and force measurements in a compact fiber optical tweezers system. In addition, we achieved more reliable signal acquisition with less distortion compared with objective based position detection methods, thanks to the light guiding in optical fibers and small distance between the fiber tips and trapped particle. As a demonstration of the fiber based detection, we used the fiber optical tweezers to apply a force on a cell membrane and simultaneously measure the cellular response.

Detection of cancerous cervical cells using physical adhesion of fluorescent silica particles and centripetal force.

PubMed

Gaikwad, Ravi M; Dokukin, Maxim E; Iyer, K Swaminathan; Woodworth, Craig D; Volkov, Dmytro O; Sokolov, Igor

2011-04-07

Here we describe a non-traditional method to identify cancerous human cervical epithelial cells in a culture dish based on physical adhesion between silica beads and cells. It is a simple optical fluorescence-based technique which detects the relative difference in the amount of fluorescent silica beads physically adherent to surfaces of cancerous and normal cervical cells. The method utilizes the centripetal force gradient that occurs in a rotating culture dish. Due to the variation in the balance between adhesion and centripetal forces, cancerous and normal cells demonstrate clearly distinctive distributions of the fluorescent particles adherent to the cell surface over the culture dish. The method demonstrates higher adhesion of silica particles to normal cells compared to cancerous cells. The difference in adhesion was initially observed by atomic force microscopy (AFM). The AFM data were used to design the parameters of the rotational dish experiment. The optical method that we describe is much faster and technically simpler than AFM. This work provides proof of the concept that physical interactions can be used to accurately discriminate normal and cancer cells. © The Royal Society of Chemistry 2011

INNOVATIVE TECHNOLOGY EVALUATION REPORT: RADIO FREQUENCY HEATING, KAI TECHNOLOGIES, INC.

EPA Science Inventory

A demonstration of KAI Technologies in-situ radio frequency heating system for soil treatment was conducted from January 1994 to July 1994 at Kelly Air Force Base in San Antonio, Texas. This demonstration was conducted as a joint effort between the USEPA and the USAF. The technol...

Standardized Testing in Physics via the World Wide Web.

ERIC Educational Resources Information Center

MacIsaac, Dan; Cole, Rebecca Pollard; Cole, David M.; McCullough, Laura; Maxka, Jim

2002-01-01

Examines the differences in paper-based and web-based administrations of a commonly used assessment instrument, the Force Concept Inventory (FCI). Results demonstrated no appreciable difference on FCI scores or FCI items based on the type of administration. Concludes that the web-based administration of the FCI appears to be as efficacious as the…

Combining single-molecule manipulation and single-molecule detection.

PubMed

Cordova, Juan Carlos; Das, Dibyendu Kumar; Manning, Harris W; Lang, Matthew J

2014-10-01

Single molecule force manipulation combined with fluorescence techniques offers much promise in revealing mechanistic details of biomolecular machinery. Here, we review force-fluorescence microscopy, which combines the best features of manipulation and detection techniques. Three of the mainstay manipulation methods (optical traps, magnetic traps and atomic force microscopy) are discussed with respect to milestones in combination developments, in addition to highlight recent contributions to the field. An overview of additional strategies is discussed, including fluorescence based force sensors for force measurement in vivo. Armed with recent exciting demonstrations of this technology, the field of combined single-molecule manipulation and single-molecule detection is poised to provide unprecedented views of molecular machinery. Copyright © 2014 Elsevier Ltd. All rights reserved.

Force-controlled automatic microassembly of tissue engineering scaffolds

NASA Astrophysics Data System (ADS)

Zhao, Guoyong; Teo, Chee Leong; Hutmacher, Dietmar Werner; Burdet, Etienne

2010-03-01

This paper presents an automated system for 3D assembly of tissue engineering (TE) scaffolds made from biocompatible microscopic building blocks with relatively large fabrication error. It focuses on the pin-into-hole force control developed for this demanding microassembly task. A beam-like gripper with integrated force sensing at a 3 mN resolution with a 500 mN measuring range is designed, and is used to implement an admittance force-controlled insertion using commercial precision stages. Visual-based alignment followed by an insertion is complemented by a haptic exploration strategy using force and position information. The system demonstrates fully automated construction of TE scaffolds with 50 microparts whose dimension error is larger than 5%.

Acoustophoretic separation of airborne millimeter-size particles by a Fresnel lens.

PubMed

Cicek, Ahmet; Korozlu, Nurettin; Adem Kaya, Olgun; Ulug, Bulent

2017-03-02

We numerically demonstrate acoustophoretic separation of spherical solid particles in air by means of an acoustic Fresnel lens. Beside gravitational and drag forces, freely-falling millimeter-size particles experience large acoustic radiation forces around the focus of the lens, where interplay of forces lead to differentiation of particle trajectories with respect to either size or material properties. Due to the strong acoustic field at the focus, radiation force can divert particles with source intensities significantly smaller than those required for acoustic levitation in a standing field. When the lens is designed to have a focal length of 100 mm at 25 kHz, finite-element method simulations reveal a sharp focus with a full-width at half-maximum of 0.5 wavelenghts and a field enhancement of 18 dB. Through numerical calculation of forces and simulation of particle trajectories, we demonstrate size-based separation of acrylic particles at a source sound pressure level of 153 dB such that particles with diameters larger than 0.5 mm are admitted into the central hole, whereas smaller particles are rejected. Besides, efficient separation of particles with similar acoustic properties such as polyethylene, polystyrene and acrylic particles of the same size is also demonstrated.

Acoustophoretic separation of airborne millimeter-size particles by a Fresnel lens

NASA Astrophysics Data System (ADS)

Cicek, Ahmet; Korozlu, Nurettin; Adem Kaya, Olgun; Ulug, Bulent

2017-03-01

We numerically demonstrate acoustophoretic separation of spherical solid particles in air by means of an acoustic Fresnel lens. Beside gravitational and drag forces, freely-falling millimeter-size particles experience large acoustic radiation forces around the focus of the lens, where interplay of forces lead to differentiation of particle trajectories with respect to either size or material properties. Due to the strong acoustic field at the focus, radiation force can divert particles with source intensities significantly smaller than those required for acoustic levitation in a standing field. When the lens is designed to have a focal length of 100 mm at 25 kHz, finite-element method simulations reveal a sharp focus with a full-width at half-maximum of 0.5 wavelenghts and a field enhancement of 18 dB. Through numerical calculation of forces and simulation of particle trajectories, we demonstrate size-based separation of acrylic particles at a source sound pressure level of 153 dB such that particles with diameters larger than 0.5 mm are admitted into the central hole, whereas smaller particles are rejected. Besides, efficient separation of particles with similar acoustic properties such as polyethylene, polystyrene and acrylic particles of the same size is also demonstrated.

Acoustophoretic separation of airborne millimeter-size particles by a Fresnel lens

PubMed Central

Cicek, Ahmet; Korozlu, Nurettin; Adem Kaya, Olgun; Ulug, Bulent

2017-01-01

We numerically demonstrate acoustophoretic separation of spherical solid particles in air by means of an acoustic Fresnel lens. Beside gravitational and drag forces, freely-falling millimeter-size particles experience large acoustic radiation forces around the focus of the lens, where interplay of forces lead to differentiation of particle trajectories with respect to either size or material properties. Due to the strong acoustic field at the focus, radiation force can divert particles with source intensities significantly smaller than those required for acoustic levitation in a standing field. When the lens is designed to have a focal length of 100 mm at 25 kHz, finite-element method simulations reveal a sharp focus with a full-width at half-maximum of 0.5 wavelenghts and a field enhancement of 18 dB. Through numerical calculation of forces and simulation of particle trajectories, we demonstrate size-based separation of acrylic particles at a source sound pressure level of 153 dB such that particles with diameters larger than 0.5 mm are admitted into the central hole, whereas smaller particles are rejected. Besides, efficient separation of particles with similar acoustic properties such as polyethylene, polystyrene and acrylic particles of the same size is also demonstrated. PMID:28252033

Graded-index fiber tip optical tweezers: numerical simulation and trapping experiment.

PubMed

Gong, Yuan; Ye, Ai-Yan; Wu, Yu; Rao, Yun-Jiang; Yao, Yao; Xiao, Song

2013-07-01

Optical fiber tweezers based on a graded-index multimode fiber (GIMMF) tip is proposed. Light propagation characteristics and gradient force distribution near the GIMMF tip are numerically investigated, which are further compared with that of optical fiber tips based on conventional single mode fibers. The simulated results indicated that by selecting optimal GIMMF length, the gradient force of the GIMMF tip tweezers is about 4 times higher than that of the SMF tip tweezers with a same shape. To prove the feasibility of such a new concept, optical trapping of yeast cells with a diameter of ~5 μm using the chemically-etched GIMMF tip is experimentally demonstrated and the trapping force is also calculated.

Dilemmas in Forward Basing: Understanding the Impact of the American Military Presence in Bahrain

DTIC Science & Technology

2014-06-01

bases, local businessmen, a base workers group, and members of the self -defense force.75 Ultimately, Governor, Ota was voted out of office and a...U.S. access to Turkish soil in 1979 to rescue hostages in Tehran during the Islamic Revolution in Iran. Relations did not begin to heal until after...demonstrators from the roundabout with rubber bullets, and teargas. Four demonstrators were killed during the raid and others died shortly after. The

Analysis of brute-force break-ins of a palmprint authentication system.

PubMed

Kong, Adams W K; Zhang, David; Kamel, Mohamed

2006-10-01

Biometric authentication systems are widely applied because they offer inherent advantages over classical knowledge-based and token-based personal-identification approaches. This has led to the development of products using palmprints as biometric traits and their use in several real applications. However, as biometric systems are vulnerable to replay, database, and brute-force attacks, such potential attacks must be analyzed before biometric systems are massively deployed in security systems. This correspondence proposes a projected multinomial distribution for studying the probability of successfully using brute-force attacks to break into a palmprint system. To validate the proposed model, we have conducted a simulation. Its results demonstrate that the proposed model can accurately estimate the probability. The proposed model indicates that it is computationally infeasible to break into the palmprint system using brute-force attacks.

Fractal based complexity measure and variation in force during sustained isometric muscle contraction: effect of aging.

PubMed

Arjunan, Sridhar P; Kumar, Dinesh K; Bastos, Teodiano

2012-01-01

This study has investigated the effect of age on the fractal based complexity measure of muscle activity and variance in the force of isometric muscle contraction. Surface electromyogram (sEMG) and force of muscle contraction were recorded from 40 healthy subjects categorized into: Group 1: Young - age range 20-30; 10 Males and 10 Females, Group 2: Old - age range 55-70; 10 Males and 10 Females during isometric exercise at Maximum Voluntary contraction (MVC). The results show that there is a reduction in the complexity of surface electromyogram (sEMG) associated with aging. The results demonstrate that there is an increase in the coefficient of variance (CoV) of the force of muscle contraction and a decrease in complexity of sEMG for the Old age group when compared with the Young age group.

Design and damping force characterization of a new magnetorheological damper activated by permanent magnet flux dispersion

NASA Astrophysics Data System (ADS)

Lee, Tae-Hoon; Han, Chulhee; Choi, Seung-Bok

2018-01-01

This work proposes a novel type of tunable magnetorheological (MR) damper operated based solely on the location of a permanent magnet incorporated into the piston. To create a larger damping force variation in comparison with the previous model, a different design configuration of the permanent-magnet-based MR (PMMR) damper is introduced to provide magnetic flux dispersion in two magnetic circuits by utilizing two materials with different magnetic reluctance. After discussing the design configuration and some advantages of the newly designed mechanism, the magnetic dispersion principle is analyzed through both the formulated analytical model of the magnetic circuit and the computer simulation based on the magnetic finite element method. Sequentially, the principal design parameters of the damper are determined and fabricated. Then, experiments are conducted to evaluate the variation in damping force depending on the location of the magnet. It is demonstrated that the new design and magnetic dispersion concept are valid showing higher damping force than the previous model. In addition, a curved structure of the two materials is further fabricated and tested to realize the linearity of the damping force variation.

Solid-phase based on-chip DNA purification through a valve-free stepwise injection of multiple reagents employing centrifugal force combined with a hydrophobic capillary barrier pressure.

PubMed

Zhang, Hainan; Tran, Hong Hanh; Chung, Bong Hyun; Lee, Nae Yoon

2013-03-21

In this paper, we demonstrate a simple technique for sequentially introducing multiple sample liquids into microchannels driven by centrifugal force combined with a hydrophobic barrier pressure and apply the technique for performing solid-phase based on-chip DNA purification. Three microchannels with varying widths, all equipped with independent sample reservoirs at the inlets, were fabricated on a hydrophobic elastomer, poly(dimethylsiloxane) (PDMS). First, glass beads were packed inside the reaction chamber, and a whole cell containing the DNA extract was introduced into the widest channel by applying centrifugal force for physical adsorption of the DNA onto the glass beads. Next, washing and elution solutions were sequentially introduced into the intermediate and narrowest microchannels, respectively, by gradually increasing the amount of centrifugal force. Through a precise manipulation of the centrifugal force, the DNA adsorbed onto the glass beads was successfully washed and eluted in a continuous manner without the need to introduce each solution manually. A stepwise injection of liquids was successfully demonstrated using multiple ink solutions, the results of which corresponded well with the theoretical analyses. As a practical application, the D1S80 locus of human genomic DNA, which is widely used for forensic purposes, was successfully purified using the microdevice introduced in this study, as demonstrated through successful target amplification. This will pave the way for the construction of a control-free valve system for realizing on-chip DNA purification, which is one of the most labor-intensive and hard-to-miniaturize components, on a greatly simplified and miniaturized platform employing hydrophobic PDMS.

Estimation of single plane unbalance parameters of a rotor-bearing system using Kalman filtering based force estimation technique

NASA Astrophysics Data System (ADS)

Shrivastava, Akash; Mohanty, A. R.

2018-03-01

This paper proposes a model-based method to estimate single plane unbalance parameters (amplitude and phase angle) in a rotor using Kalman filter and recursive least square based input force estimation technique. Kalman filter based input force estimation technique requires state-space model and response measurements. A modified system equivalent reduction expansion process (SEREP) technique is employed to obtain a reduced-order model of the rotor system so that limited response measurements can be used. The method is demonstrated using numerical simulations on a rotor-disk-bearing system. Results are presented for different measurement sets including displacement, velocity, and rotational response. Effects of measurement noise level, filter parameters (process noise covariance and forgetting factor), and modeling error are also presented and it is observed that the unbalance parameter estimation is robust with respect to measurement noise.

Damage localization and quantification of composite stratified beam Structures using residual force method

NASA Astrophysics Data System (ADS)

Behtani, A.; Bouazzouni, A.; Khatir, S.; Tiachacht, S.; Zhou, Y.-L.; Abdel Wahab, M.

2017-05-01

In this paper, the problem of using measured modal parameters to detect and locate damage in beam composite stratified structures with four layers of graphite/epoxy [0°/902°/0°] is investigated. A technique based on the residual force method is applied to composite stratified structure with different boundary conditions, the results of damage detection for several damage cases demonstrate that using residual force method as damage index, the damage location can be identified correctly and the damage extents can be estimated as well.

A digital beamforming processor for the joint DoD/NASA space based radar mission

NASA Technical Reports Server (NTRS)

Fischman, Mark A.; Le, Charles; Rosen, Paul A.

2004-01-01

The Space Based Radar (SBR) program includes a joint technology demonstration between NASA and the Air Force to design a low-earth orbiting, 2x50 m L-band radar system for both Earth science and intelligence related observations.

Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality.

PubMed

Carvalho, Filomena A; Martins, Ivo C; Santos, Nuno C

2013-03-01

Atomic force microscopy (AFM) applied to biological systems can, besides generating high-quality and well-resolved images, be employed to study protein folding via AFM-based force spectroscopy. This approach allowed remarkable advances in the measurement of inter- and intramolecular interaction forces with piconewton resolution. The detection of specific interaction forces between molecules based on the AFM sensitivity and the manipulation of individual molecules greatly advanced the understanding of intra-protein and protein-ligand interactions. Apart from the academic interest in the resolution of basic scientific questions, this technique has also key importance on the clarification of several biological questions of immediate biomedical relevance. Force spectroscopy is an especially appropriate technique for "mechanical proteins" that can provide crucial information on single protein molecules and/or domains. Importantly, it also has the potential of combining in a single experiment spatial and kinetic measurements. Here, the main principles of this methodology are described, after which the ability to measure interactions at the single-molecule level is discussed, in the context of relevant protein-folding examples. We intend to demonstrate the potential of AFM-based force spectroscopy in the study of protein folding, especially since this technique is able to circumvent some of the difficulties typically encountered in classical thermal/chemical denaturation studies. Copyright © 2012 Elsevier Inc. All rights reserved.

Model-based traction force microscopy reveals differential tension in cellular actin bundles.

PubMed

Soiné, Jérôme R D; Brand, Christoph A; Stricker, Jonathan; Oakes, Patrick W; Gardel, Margaret L; Schwarz, Ulrich S

2015-03-01

Adherent cells use forces at the cell-substrate interface to sense and respond to the physical properties of their environment. These cell forces can be measured with traction force microscopy which inverts the equations of elasticity theory to calculate them from the deformations of soft polymer substrates. We introduce a new type of traction force microscopy that in contrast to traditional methods uses additional image data for cytoskeleton and adhesion structures and a biophysical model to improve the robustness of the inverse procedure and abolishes the need for regularization. We use this method to demonstrate that ventral stress fibers of U2OS-cells are typically under higher mechanical tension than dorsal stress fibers or transverse arcs.

Model-based Traction Force Microscopy Reveals Differential Tension in Cellular Actin Bundles

PubMed Central

Soiné, Jérôme R. D.; Brand, Christoph A.; Stricker, Jonathan; Oakes, Patrick W.; Gardel, Margaret L.; Schwarz, Ulrich S.

2015-01-01

Adherent cells use forces at the cell-substrate interface to sense and respond to the physical properties of their environment. These cell forces can be measured with traction force microscopy which inverts the equations of elasticity theory to calculate them from the deformations of soft polymer substrates. We introduce a new type of traction force microscopy that in contrast to traditional methods uses additional image data for cytoskeleton and adhesion structures and a biophysical model to improve the robustness of the inverse procedure and abolishes the need for regularization. We use this method to demonstrate that ventral stress fibers of U2OS-cells are typically under higher mechanical tension than dorsal stress fibers or transverse arcs. PMID:25748431

Fiber based optical tweezers for simultaneous in situ force exertion and measurements in a 3D polyacrylamide gel compartment.

PubMed

Ti, Chaoyang; Thomas, Gawain M; Ren, Yundong; Zhang, Rui; Wen, Qi; Liu, Yuxiang

2015-07-01

Optical tweezers play an important role in biological applications. However, it is difficult for traditional optical tweezers based on objective lenses to work in a three-dimensional (3D) solid far away from the substrate. In this work, we develop a fiber based optical trapping system, namely inclined dual fiber optical tweezers, that can simultaneously apply and measure forces both in water and in a 3D polyacrylamide gel matrix. In addition, we demonstrate in situ, non-invasive characterization of local mechanical properties of polyacrylamide gel by measurements on an embedded bead. The fiber optical tweezers measurements agree well with those of atomic force microscopy (AFM). The inclined dual fiber optical tweezers provide a promising and versatile tool for cell mechanics study in 3D environments.

Piezoresistive cantilever force-clamp system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, Sung-Jin; Petzold, Bryan C.; Pruitt, Beth L.

2011-04-15

We present a microelectromechanical device-based tool, namely, a force-clamp system that sets or ''clamps'' the scaled force and can apply designed loading profiles (e.g., constant, sinusoidal) of a desired magnitude. The system implements a piezoresistive cantilever as a force sensor and the built-in capacitive sensor of a piezoelectric actuator as a displacement sensor, such that sample indentation depth can be directly calculated from the force and displacement signals. A programmable real-time controller operating at 100 kHz feedback calculates the driving voltage of the actuator. The system has two distinct modes: a force-clamp mode that controls the force applied to amore » sample and a displacement-clamp mode that controls the moving distance of the actuator. We demonstrate that the system has a large dynamic range (sub-nN up to tens of {mu}N force and nm up to tens of {mu}m displacement) in both air and water, and excellent dynamic response (fast response time, <2 ms and large bandwidth, 1 Hz up to 1 kHz). In addition, the system has been specifically designed to be integrated with other instruments such as a microscope with patch-clamp electronics. We demonstrate the capabilities of the system by using it to calibrate the stiffness and sensitivity of an electrostatic actuator and to measure the mechanics of a living, freely moving Caenorhabditis elegans nematode.« less

Piezoresistive cantilever force-clamp system

PubMed Central

Park, Sung-Jin; Petzold, Bryan C.; Goodman, Miriam B.; Pruitt, Beth L.

2011-01-01

We present a microelectromechanical device-based tool, namely, a force-clamp system that sets or “clamps” the scaled force and can apply designed loading profiles (e.g., constant, sinusoidal) of a desired magnitude. The system implements a piezoresistive cantilever as a force sensor and the built-in capacitive sensor of a piezoelectric actuator as a displacement sensor, such that sample indentation depth can be directly calculated from the force and displacement signals. A programmable real-time controller operating at 100 kHz feedback calculates the driving voltage of the actuator. The system has two distinct modes: a force-clamp mode that controls the force applied to a sample and a displacement-clamp mode that controls the moving distance of the actuator. We demonstrate that the system has a large dynamic range (sub-nN up to tens of μN force and nm up to tens of μm displacement) in both air and water, and excellent dynamic response (fast response time, <2 ms and large bandwidth, 1 Hz up to 1 kHz). In addition, the system has been specifically designed to be integrated with other instruments such as a microscope with patch-clamp electronics. We demonstrate the capabilities of the system by using it to calibrate the stiffness and sensitivity of an electrostatic actuator and to measure the mechanics of a living, freely moving Caenorhabditis elegans nematode. PMID:21529009

Difference in peak weight transfer and timing based on golf handicap.

PubMed

Queen, Robin M; Butler, Robert J; Dai, Boyi; Barnes, C Lowry

2013-09-01

Weight shift during the golf swing has been a topic of discussion among golf professionals; however, it is still unclear how weight shift varies in golfers of different performance levels. The main purpose of this study was to examine the following: (a) the changes in the peak ground reaction forces (GRF) and the timing of these events between high (HHCP) and low handicap (LHCP) golfers and (b) the differences between the leading and trailing legs. Twenty-eight male golfers were recruited and divided based on having an LHCP < 9 or HHCP > 9. Three-dimensional GRF peaks and the timing of the peaks were recorded bilaterally during a golf swing. The golf swing was divided into different phases: (a) address to the top of the backswing, (b) top of the backswing to ball contact, and (c) ball contact to the end of follow through. Repeated measures analyses of variance (α = 0.05) were completed for each study variable: the magnitude and the timing of peak vertical GRF, peak lateral GRF, and peak medial GRF (α = 0.05). The LHCP group had a greater transfer of vertical force from the trailing foot to the leading foot in phase 2 than the HHCP group. The LHCP group also demonstrated earlier timing of peak vertical force throughout the golf swing than the HHCP group. The LHCP and HHCP groups demonstrated different magnitudes of peak lateral force. The LHCP group had an earlier timing of peak lateral GRF in phase 2 and earlier timing of peak medial GRF in phases 1 and 2 than the HHCP group. In general, LHCP golfers demonstrated greater and earlier force generation than HHCP golfers. It may be relevant to consider both the magnitude of the forces and the timing of these events during golf-specific training to improve performance. These data reveal weight shifting differences that can be addressed by teaching professionals to help their students better understand weight transfer during the golf swing to optimize performance.

A micromachined membrane-based active probe for biomolecular mechanics measurement

NASA Astrophysics Data System (ADS)

Torun, H.; Sutanto, J.; Sarangapani, K. K.; Joseph, P.; Degertekin, F. L.; Zhu, C.

2007-04-01

A novel micromachined, membrane-based probe has been developed and fabricated as assays to enable parallel measurements. Each probe in the array can be individually actuated, and the membrane displacement can be measured with high resolution using an integrated diffraction-based optical interferometer. To illustrate its application in single-molecule mechanics experiments, this membrane probe was used to measure unbinding forces between L-selectin reconstituted in a polymer-cushioned lipid bilayer on the probe membrane and an antibody adsorbed on an atomic force microscope cantilever. Piconewton range forces between single pairs of interacting molecules were measured from the cantilever bending while using the membrane probe as an actuator. The integrated diffraction-based optical interferometer of the probe was demonstrated to have <10 fm Hz-1/2 noise floor for frequencies as low as 3 Hz with a differential readout scheme. With soft probe membranes, this low noise level would be suitable for direct force measurements without the need for a cantilever. Furthermore, the probe membranes were shown to have 0.5 µm actuation range with a flat response up to 100 kHz, enabling measurements at fast speeds.

Switching sliding mode force tracking control of piezoelectric-hydraulic pump-based friction element actuation systems for automotive transmissions

NASA Astrophysics Data System (ADS)

Kim, Gi-Woo; Wang, K. W.

2009-08-01

In this study, a nonlinear sliding-mode controller is designed for force tracking of a piezoelectric-hydraulic pump (PHP)-based actuation system, which is developed to replace the current electro-hydraulic actuation systems for automatic transmission (AT) friction elements, such as band brakes or clutches. By utilizing the PHP, one can eliminate the various hydraulic components (oil pump, regulating valve and control valve) in current ATs and achieve a simpler configuration with more efficient operation. With the derived governing equation of motion of the PHP-based actuation system integrated with the friction element (band brake), a switching control law is synthesized based on the sliding-mode theory. To evaluate the effectiveness of the proposed control law, its force tracking performance for the engagement of a friction element during an AT 1\\to 2 up-shift is examined experimentally. It is shown that one can successfully track the desired force trajectory for AT shift control with small tracking error. This study demonstrates the potential of the PHP as a new controllable actuation system for AT friction elements.

Selective particle trapping using an oscillating microbubble.

PubMed

Rogers, Priscilla; Neild, Adrian

2011-11-07

The ability to isolate and sort analytes within complex microfluidic volumes is essential to the success of lab-on-a-chip (LOC) devices. In this study, acoustically-excited oscillating bubbles are used to selectively trap particles, with the selectivity being a function of both particle size and density. The operating principle is based on the interplay between the strong microstreaming-induced drag force and the attractive secondary Bjerknes force. Depending upon the size of the bubble, and thus its resonant frequency, it is possible to cause one force to dominate over the other, resulting in either particle attraction or repulsion. A theoretical analysis reveals the extent of the contribution of each force for a given particle size; in close agreement with experimental findings. Density-based trapping is also demonstrated, highlighting that denser particles experience a larger secondary Bjerknes force resulting in their attraction. This study showcases the excellent applicability and versatility of using oscillating bubbles as a trapping and sorting mechanism within LOC devices. This journal is © The Royal Society of Chemistry 2011

The design and implementation of a windowing interface pinch force measurement system

NASA Astrophysics Data System (ADS)

Ho, Tze-Yee; Chen, Yuanu-Joan; Chung, Chin-Teng; Hsiao, Ming-Heng

2010-02-01

This paper presents a novel windowing interface pinch force measurement system that is basically based on an USB (Universal Series Bus) microcontroller which mainly processes the sensing data from the force sensing resistance sensors mounted on five digits. It possesses several friendly functions, such as the value and curve trace of the applied force by a hand injured patient displayed in real time on a monitoring screen, consequently, not only the physician can easily evaluate the effect of hand injury rehabilitation, but also the patients get more progressive during the hand physical therapy by interacting with the screen of pinch force measurement. In order to facilitate the pinch force measurement system and make it friendly, the detail hardware design and software programming flowchart are described in this paper. Through a series of carefully and detailed experimental tests, first of all, the relationship between the applying force and the FSR sensors are measured and verified. Later, the different type of pinch force measurements are verified by the oscilloscope and compared with the corresponding values and waveform traces in the window interface display panel to obtain the consistency. Finally, a windowing interface pinch force measurement system based on the USB microcontroller is implemented and demonstrated. The experimental results show the verification and feasibility of the designed system.

Quantification of surface displacements and electromechanical phenomena via dynamic atomic force microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Balke, Nina; Jesse, Stephen; Yu, Pu

Detection of dynamic surface displacements associated with local changes in material strain provides access to a number of phenomena and material properties. Contact resonance-enhanced methods of atomic force microscopy (AFM) have been shown capable of detecting ~1–3 pm-level surface displacements, an approach used in techniques such as piezoresponse force microscopy, atomic force acoustic microscopy, and ultrasonic force microscopy. Here, based on an analytical model of AFM cantilever vibrations, we demonstrate a guideline to quantify surface displacements with high accuracy by taking into account the cantilever shape at the first resonant contact mode, depending on the tip–sample contact stiffness. The approachmore » has been experimentally verified and further developed for piezoresponse force microscopy (PFM) using well-defined ferroelectric materials. These results open up a way to accurate and precise measurements of surface displacement as well as piezoelectric constants at the pm-scale with nanometer spatial resolution and will allow avoiding erroneous data interpretations and measurement artifacts. Furthermore, this analysis is directly applicable to all cantilever-resonance-based scanning probe microscopy (SPM) techniques.« less

Quantification of surface displacements and electromechanical phenomena via dynamic atomic force microscopy

DOE PAGES

Balke, Nina; Jesse, Stephen; Yu, Pu; ...

2016-09-15

Detection of dynamic surface displacements associated with local changes in material strain provides access to a number of phenomena and material properties. Contact resonance-enhanced methods of atomic force microscopy (AFM) have been shown capable of detecting ~1–3 pm-level surface displacements, an approach used in techniques such as piezoresponse force microscopy, atomic force acoustic microscopy, and ultrasonic force microscopy. Here, based on an analytical model of AFM cantilever vibrations, we demonstrate a guideline to quantify surface displacements with high accuracy by taking into account the cantilever shape at the first resonant contact mode, depending on the tip–sample contact stiffness. The approachmore » has been experimentally verified and further developed for piezoresponse force microscopy (PFM) using well-defined ferroelectric materials. These results open up a way to accurate and precise measurements of surface displacement as well as piezoelectric constants at the pm-scale with nanometer spatial resolution and will allow avoiding erroneous data interpretations and measurement artifacts. Furthermore, this analysis is directly applicable to all cantilever-resonance-based scanning probe microscopy (SPM) techniques.« less

KSC-08pd1345

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- A representative of the 301st Rescue Squadron demonstrates rescue equipment that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base, Fla. In the background is an HH-60G helicopter. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

KSC-08pd1351

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- Representatives of the 301st Rescue Squadron demonstrate the use of rescue equipment on the HH-60G helicopter that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base, Fla. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

KSC-08pd1350

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- A representative of the 301st Rescue Squadron demonstrates rescue equipment on the HH-60G helicopter that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base, Fla. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

KSC-08pd1349

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- Representatives of the 301st Rescue Squadron demonstrate the use of rescue equipment on the HH-60G helicopter that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base, Fla. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

KSC-08pd1344

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- A representative of the 301st Rescue Squadron demonstrates rescue equipment that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base, Fla. In the background is an HH-60G helicopter. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

Turbine blade forced response prediction using FREPS

NASA Technical Reports Server (NTRS)

Murthy, Durbha, V.; Morel, Michael R.

1993-01-01

This paper describes a software system called FREPS (Forced REsponse Prediction System) that integrates structural dynamic, steady and unsteady aerodynamic analyses to efficiently predict the forced response dynamic stresses in axial flow turbomachinery blades due to aerodynamic and mechanical excitations. A flutter analysis capability is also incorporated into the system. The FREPS system performs aeroelastic analysis by modeling the motion of the blade in terms of its normal modes. The structural dynamic analysis is performed by a finite element code such as MSC/NASTRAN. The steady aerodynamic analysis is based on nonlinear potential theory and the unsteady aerodynamic analyses is based on the linearization of the non-uniform potential flow mean. The program description and presentation of the capabilities are reported herein. The effectiveness of the FREPS package is demonstrated on the High Pressure Oxygen Turbopump turbine of the Space Shuttle Main Engine. Both flutter and forced response analyses are performed and typical results are illustrated.

KSC-08pd1346

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- A representative of the 301st Rescue Squadron demonstrates rescue equipment that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base, Fla. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

Nonlinear force feedback control of piezoelectric-hydraulic pump actuator for automotive transmission shift control

NASA Astrophysics Data System (ADS)

Kim, Gi-Woo; Wang, K. W.

2008-03-01

In recent years, researchers have investigated the feasibility of utilizing piezoelectric-hydraulic pump based actuation systems for automotive transmission controls. This new concept could eventually reduce the complexity, weight, and fuel consumption of the current transmissions. In this research, we focus on how to utilize this new approach on the shift control of automatic transmissions (AT), which generally requires pressure profiling for friction elements during the operation. To illustrate the concept, we will consider the 1--> 2 up shift control using band brake friction elements. In order to perform the actuation force tracking for AT shift control, nonlinear force feedback control laws are designed based on the sliding mode theory for the given nonlinear system. This paper will describe the modeling of the band brake actuation system, the design of the nonlinear force feedback controller, and simulation and experimental results for demonstration of the new concept.

Multifunctional hydrogel nano-probes for atomic force microscopy

PubMed Central

Lee, Jae Seol; Song, Jungki; Kim, Seong Oh; Kim, Seokbeom; Lee, Wooju; Jackman, Joshua A.; Kim, Dongchoul; Cho, Nam-Joon; Lee, Jungchul

2016-01-01

Since the invention of the atomic force microscope (AFM) three decades ago, there have been numerous advances in its measurement capabilities. Curiously, throughout these developments, the fundamental nature of the force-sensing probe—the key actuating element—has remained largely unchanged. It is produced by long-established microfabrication etching strategies and typically composed of silicon-based materials. Here, we report a new class of photopolymerizable hydrogel nano-probes that are produced by bottom-up fabrication with compressible replica moulding. The hydrogel probes demonstrate excellent capabilities for AFM imaging and force measurement applications while enabling programmable, multifunctional capabilities based on compositionally adjustable mechanical properties and facile encapsulation of various nanomaterials. Taken together, the simple, fast and affordable manufacturing route and multifunctional capabilities of hydrogel AFM nano-probes highlight the potential of soft matter mechanical transducers in nanotechnology applications. The fabrication scheme can also be readily utilized to prepare hydrogel cantilevers, including in parallel arrays, for nanomechanical sensor devices. PMID:27199165

Current-induced changes of migration energy barriers in graphene and carbon nanotubes

NASA Astrophysics Data System (ADS)

Obodo, J. T.; Rungger, I.; Sanvito, S.; Schwingenschlögl, U.

2016-05-01

An electron current can move atoms in a nanoscale device with important consequences for the device operation and breakdown. We perform first principles calculations aimed at evaluating the possibility of changing the energy barriers for atom migration in carbon-based systems. In particular, we consider the migration of adatoms and defects in graphene and carbon nanotubes. Although the current-induced forces are large for both the systems, in graphene the force component along the migration path is small and therefore the barrier height is little affected by the current flow. In contrast, the same barrier is significantly reduced in carbon nanotubes as the current increases. Our work also provides a real-system numerical demonstration that current-induced forces within density functional theory are non-conservative.An electron current can move atoms in a nanoscale device with important consequences for the device operation and breakdown. We perform first principles calculations aimed at evaluating the possibility of changing the energy barriers for atom migration in carbon-based systems. In particular, we consider the migration of adatoms and defects in graphene and carbon nanotubes. Although the current-induced forces are large for both the systems, in graphene the force component along the migration path is small and therefore the barrier height is little affected by the current flow. In contrast, the same barrier is significantly reduced in carbon nanotubes as the current increases. Our work also provides a real-system numerical demonstration that current-induced forces within density functional theory are non-conservative. Electronic supplementary information (ESI) available. See DOI: 10.1039/C6NR00534A

Evaluating learning and teaching using the Force Concept Inventory

NASA Astrophysics Data System (ADS)

Zitzewitz, Paul

1997-04-01

Teaching methods used in the calculus-based mechanics course for engineers and scientists (P150) at the University of Michigan-Dearborn were markedly changed in September, 1996. Lectures emphasize active learning with Mazur's ConcepTests, Sokoloff's Interactive Demonstrations, and Van Heuvelen's ALPS Kit worksheets. Students solve context-rich problems using Van Heuvelen's multiple representation format in cooperative groups in discussion sections. Labs were changed to use MBL emphasizing concepts and Experiment Problems to learn lab-based problem solving. Pre- and post-testing of 400 students with the Force Concept Inventory has demonstrated considerable success. The average increase in score has been 35-45methods as defined by Hake. The methods and results will be discussed. Detailed analyses of the FCI results will look at success in teaching specific concepts and the effect of student preparation in mathematics and high school physics.

On the use of satellite-based estimates of rainfall temporal distribution to simulate the potential for malaria transmission in rural Africa

NASA Astrophysics Data System (ADS)

Yamana, Teresa K.; Eltahir, Elfatih A. B.

2011-02-01

This paper describes the use of satellite-based estimates of rainfall to force the Hydrology, Entomology and Malaria Transmission Simulator (HYDREMATS), a hydrology-based mechanistic model of malaria transmission. We first examined the temporal resolution of rainfall input required by HYDREMATS. Simulations conducted over Banizoumbou village in Niger showed that for reasonably accurate simulation of mosquito populations, the model requires rainfall data with at least 1 h resolution. We then investigated whether HYDREMATS could be effectively forced by satellite-based estimates of rainfall instead of ground-based observations. The Climate Prediction Center morphing technique (CMORPH) precipitation estimates distributed by the National Oceanic and Atmospheric Administration are available at a 30 min temporal resolution and 8 km spatial resolution. We compared mosquito populations simulated by HYDREMATS when the model is forced by adjusted CMORPH estimates and by ground observations. The results demonstrate that adjusted rainfall estimates from satellites can be used with a mechanistic model to accurately simulate the dynamics of mosquito populations.

A Replicable, Zero-Based Model for Marketing Curriculum Innovation

ERIC Educational Resources Information Center

Borin, Norm; Metcalf, Lynn E.; Tietje, Brian C.

2007-01-01

As university curriculums inevitably change, their evolution typically occurs through a series of minor incremental adjustments to individual courses that cause the curriculum to lose strategic consistency and focus. This article demonstrates a zero-based approach to marketing curriculum innovation. The authors describe forces of change that led…

Experimental validation of a distribution theory based analysis of the effect of manufacturing tolerances on permanent magnet synchronous machines

NASA Astrophysics Data System (ADS)

Boscaino, V.; Cipriani, G.; Di Dio, V.; Corpora, M.; Curto, D.; Franzitta, V.; Trapanese, M.

2017-05-01

An experimental study on the effect of permanent magnet tolerances on the performances of a Tubular Linear Ferrite Motor is presented in this paper. The performances that have been investigated are: cogging force, end effect cogging force and generated thrust. It is demonstrated that: 1) the statistical variability of the magnets introduces harmonics in the spectrum of the cogging force; 2) the value of the end effect cogging force is directly linked to the values of then remanence field of the external magnets placed on the slider; 3) the generated thrust and its statistical distribution depend on the remanence field of the magnets placed on the translator.

Consistent forcing scheme in the cascaded lattice Boltzmann method

NASA Astrophysics Data System (ADS)

Fei, Linlin; Luo, Kai Hong

2017-11-01

In this paper, we give an alternative derivation for the cascaded lattice Boltzmann method (CLBM) within a general multiple-relaxation-time (MRT) framework by introducing a shift matrix. When the shift matrix is a unit matrix, the CLBM degrades into an MRT LBM. Based on this, a consistent forcing scheme is developed for the CLBM. The consistency of the nonslip rule, the second-order convergence rate in space, and the property of isotropy for the consistent forcing scheme is demonstrated through numerical simulations of several canonical problems. Several existing forcing schemes previously used in the CLBM are also examined. The study clarifies the relation between MRT LBM and CLBM under a general framework.

A grouping method based on grid density and relationship for crowd evacuation simulation

NASA Astrophysics Data System (ADS)

Li, Yan; Liu, Hong; Liu, Guang-peng; Li, Liang; Moore, Philip; Hu, Bin

2017-05-01

Psychological factors affect the movement of people in the competitive or panic mode of evacuation, in which the density of pedestrians is relatively large and the distance among them is small. In this paper, a crowd is divided into groups according to their social relations to simulate the actual movement of crowd evacuation more realistically and increase the attractiveness of the group based on social force model. The force of group attraction is the synthesis of two forces; one is the attraction of the individuals generated by their social relations to gather, and the other is that of the group leader to the individuals within the group to ensure that the individuals follow the leader. The synthetic force determines the trajectory of individuals. The evacuation process is demonstrated using the improved social force model. In the improved social force model, the individuals with close social relations gradually present a closer and coordinated action while following the leader. In this paper, a grouping algorithm is proposed based on grid density and relationship via computer simulation to illustrate the features of the improved social force model. The definition of the parameters involved in the algorithm is given, and the effect of relational value on the grouping is tested. Reasonable numbers of grids and weights are selected. The effectiveness of the algorithm is shown through simulation experiments. A simulation platform is also established using the proposed grouping algorithm and the improved social force model for crowd evacuation simulation.

Investigation of the heparin-thrombin interaction by dynamic force spectroscopy.

PubMed

Wang, Congzhou; Jin, Yingzi; Desai, Umesh R; Yadavalli, Vamsi K

2015-06-01

The interaction between heparin and thrombin is a vital step in the blood (anti)coagulation process. Unraveling the molecular basis of the interactions is therefore extremely important in understanding the mechanisms of this complex biological process. In this study, we use a combination of an efficient thiolation chemistry of heparin, a self-assembled monolayer-based single molecule platform, and a dynamic force spectroscopy to provide new insights into the heparin-thrombin interaction from an energy viewpoint at the molecular scale. Well-separated single molecules of heparin covalently attached to mixed self-assembled monolayers are demonstrated, whereby interaction forces with thrombin can be measured via atomic force microscopy-based spectroscopy. Further these interactions are studied at different loading rates and salt concentrations to directly obtain kinetic parameters. An increase in the loading rate shows a higher interaction force between the heparin and thrombin, which can be directly linked to the kinetic dissociation rate constant (koff). The stability of the heparin/thrombin complex decreased with increasing NaCl concentration such that the off-rate was found to be driven primarily by non-ionic forces. These results contribute to understanding the role of specific and nonspecific forces that drive heparin-thrombin interactions under applied force or flow conditions. Copyright © 2015 Elsevier B.V. All rights reserved.

Finding the cell center by a balance of dynein and myosin pulling and microtubule pushing: a computational study.

PubMed

Zhu, Jie; Burakov, Anton; Rodionov, Vladimir; Mogilner, Alex

2010-12-01

The centrosome position in many types of interphase cells is actively maintained in the cell center. Our previous work indicated that the centrosome is kept at the center by pulling force generated by dynein and actin flow produced by myosin contraction and that an unidentified factor that depends on microtubule dynamics destabilizes position of the centrosome. Here, we use modeling to simulate the centrosome positioning based on the idea that the balance of three forces-dyneins pulling along microtubule length, myosin-powered centripetal drag, and microtubules pushing on organelles-is responsible for the centrosome displacement. By comparing numerical predictions with centrosome behavior in wild-type and perturbed interphase cells, we rule out several plausible hypotheses about the nature of the microtubule-based force. We conclude that strong dynein- and weaker myosin-generated forces pull the microtubules inward competing with microtubule plus-ends pushing the microtubule aster outward and that the balance of these forces positions the centrosome at the cell center. The model also predicts that kinesin action could be another outward-pushing force. Simulations demonstrate that the force-balance centering mechanism is robust yet versatile. We use the experimental observations to reverse engineer the characteristic forces and centrosome mobility.

Frustration in protein elastic network models

NASA Astrophysics Data System (ADS)

Lezon, Timothy; Bahar, Ivet

2010-03-01

Elastic network models (ENMs) are widely used for studying the equilibrium dynamics of proteins. The most common approach in ENM analysis is to adopt a uniform force constant or a non-specific distance dependent function to represent the force constant strength. Here we discuss the influence of sequence and structure in determining the effective force constants between residues in ENMs. Using a novel method based on entropy maximization, we optimize the force constants such that they exactly reporduce a subset of experimentally determined pair covariances for a set of proteins. We analyze the optimized force constants in terms of amino acid types, distances, contact order and secondary structure, and we demonstrate that including frustrated interactions in the ENM is essential for accurately reproducing the global modes in the middle of the frequency spectrum.

A silicon-nanowire memory driven by optical gradient force induced bistability

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dong, B.; Institute of Microelectronics, A*STAR; Cai, H., E-mail: caih@ime.a-star.edu.sg

2015-12-28

In this paper, a bistable optical-driven silicon-nanowire memory is demonstrated, which employs ring resonator to generate optical gradient force over a doubly clamped silicon-nanowire. Two stable deformation positions of a doubly clamped silicon-nanowire represent two memory states (“0” and “1”) and can be set/reset by modulating the light intensity (<3 mW) based on the optical force induced bistability. The time response of the optical-driven memory is less than 250 ns. It has applications in the fields of all optical communication, quantum computing, and optomechanical circuits.

Friction damping of two-dimensional motion and its application in vibration control

NASA Technical Reports Server (NTRS)

Menq, C.-H.; Chidamparam, P.; Griffin, J. H.

1991-01-01

This paper presents an approximate method for analyzing the two-dimensional friction contact problem so as to compute the dynamic response of a structure constrained by friction interfaces. The friction force at the joint is formulated based on the Coulomb model. The single-term harmonic balance scheme, together with the receptance approach of decoupling the effect of the friction force on the structure from those of the external forces has been utilized to obtain the steady state response. The computational efficiency and accuracy of the method are demonstrated by comparing the results with long-term time solutions.

Towards Subject-Specific Strength Training Design through Predictive Use of Musculoskeletal Models.

PubMed

Plüss, Michael; Schellenberg, Florian; Taylor, William R; Lorenzetti, Silvio

2018-01-01

Lower extremity dysfunction is often associated with hip muscle strength deficiencies. Detailed knowledge of the muscle forces generated in the hip under specific external loading conditions enables specific structures to be trained. The aim of this study was to find the most effective movement type and loading direction to enable the training of specific parts of the hip muscles using a standing posture and a pulley system. In a novel approach to release the predictive power of musculoskeletal modelling techniques based on inverse dynamics, flexion/extension and ab-/adduction movements were virtually created. To demonstrate the effectiveness of this approach, three hip orientations and an external loading force that was systematically rotated around the body were simulated using a state-of-the art OpenSim model in order to establish ideal designs for training of the anterior and posterior parts of the M. gluteus medius (GM). The external force direction as well as the hip orientation greatly influenced the muscle forces in the different parts of the GM. No setting was found for simultaneous training of the anterior and posterior parts with a muscle force higher than 50% of the maximum. Importantly, this study has demonstrated the use of musculoskeletal models as an approach to predict muscle force variations for different strength and rehabilitation exercise variations.

Towards Subject-Specific Strength Training Design through Predictive Use of Musculoskeletal Models

PubMed Central

Plüss, Michael; Schellenberg, Florian

2018-01-01

Lower extremity dysfunction is often associated with hip muscle strength deficiencies. Detailed knowledge of the muscle forces generated in the hip under specific external loading conditions enables specific structures to be trained. The aim of this study was to find the most effective movement type and loading direction to enable the training of specific parts of the hip muscles using a standing posture and a pulley system. In a novel approach to release the predictive power of musculoskeletal modelling techniques based on inverse dynamics, flexion/extension and ab-/adduction movements were virtually created. To demonstrate the effectiveness of this approach, three hip orientations and an external loading force that was systematically rotated around the body were simulated using a state-of-the art OpenSim model in order to establish ideal designs for training of the anterior and posterior parts of the M. gluteus medius (GM). The external force direction as well as the hip orientation greatly influenced the muscle forces in the different parts of the GM. No setting was found for simultaneous training of the anterior and posterior parts with a muscle force higher than 50% of the maximum. Importantly, this study has demonstrated the use of musculoskeletal models as an approach to predict muscle force variations for different strength and rehabilitation exercise variations. PMID:29796082

Multidirectional flexible force sensors based on confined, self-adjusting carbon nanotube arrays

NASA Astrophysics Data System (ADS)

Lee, J.-I.; Pyo, Soonjae; Kim, Min-Ook; Kim, Jongbaeg

2018-02-01

We demonstrate a highly sensitive force sensor based on self-adjusting carbon nanotube (CNT) arrays. Aligned CNT arrays are directly synthesized on silicon microstructures by a space-confined growth technique which enables a facile self-adjusting contact. To afford flexibility and softness, the patterned microstructures with the integrated CNTs are embedded in polydimethylsiloxane structures. The sensing mechanism is based on variations in the contact resistance between the facing CNT arrays under the applied force. By finite element analysis, proper dimensions and positions for each component are determined. Further, high sensitivities up to 15.05%/mN of the proposed sensors were confirmed experimentally. Multidirectional sensing capability could also be achieved by designing multiple sets of sensing elements in a single sensor. The sensors show long-term operational stability, owing to the unique properties of the constituent CNTs, such as outstanding mechanical durability and elasticity.

Linear thermal circulator based on Coriolis forces.

PubMed

Li, Huanan; Kottos, Tsampikos

2015-02-01

We show that the presence of a Coriolis force in a rotating linear lattice imposes a nonreciprocal propagation of the phononic heat carriers. Using this effect we propose the concept of Coriolis linear thermal circulator which can control the circulation of a heat current. A simple model of three coupled harmonic masses on a rotating platform permits us to demonstrate giant circulating rectification effects for moderate values of the angular velocities of the platform.

Demonstration of Laser-Induced Fluorescence on Krypton Hall Effect Thruster

DTIC Science & Technology

2011-08-10

5b. GRANT NUMBER Thruster 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) William A. Hargus Jr., Gregory X. Azarnia, and Michael R. Nakles 5d. PROJECT... William A. Hargus, Jr. a. REPORT Unclassified b. ABSTRACT Unclassified c. THIS PAGE Unclassified SAR 13 19b. TELEPHONE NUMBER (include...Thruster William A. Hargus, Jr.∗ Gregory M. Azarnia† Michael R. Nakles‡ Air Force Research Laboratory, Edwards Air Force Base, CA 93524 There is growing

Alternatives for Modernizing U.S. Fighter Forces

DTIC Science & Technology

2009-05-01

Plans for Modernizing Fighter Forces xS -2. The Possible Role of the F-22 Program in Mitigating the Air Force’s Projected Inventory Shortfall xvi1- 1 ... Northrop Grumman won that competition with its proposal for an aircraft based on the RQ-4 Global Hawk. 38 ALTERNATIVES FOR MODERNIZING U.S. FIGHTER...flyable demonstrator for exploring technologies that could lead to an operational UCAV-N, was rolled out by Northrop Grumman in December 2008. Its

Improved finite-difference computation of the van der Waals force: One-dimensional case

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pinto, Fabrizio

2009-10-15

We present an improved demonstration of the calculation of Casimir forces in one-dimensional systems based on the recently proposed numerical imaginary frequency Green's function computation approach. The dispersion force on two thick lossy dielectric slabs separated by an empty gap and placed within a perfectly conducting cavity is obtained from the Green's function of the modified Helmholtz equation by means of an ordinary finite-difference method. In order to demonstrate the possibility to develop algorithms to explore complex geometries in two and three dimensions to higher order in the mesh spacing, we generalize existing classical electromagnetism algebraic methods to generate themore » difference equations for dielectric boundaries not coinciding with any grid points. Diagnostic tests are presented to monitor the accuracy of our implementation of the method and follow-up applications in higher dimensions are introduced.« less

Subatomic-scale force vector mapping above a Ge(001) dimer using bimodal atomic force microscopy

NASA Astrophysics Data System (ADS)

Naitoh, Yoshitaka; Turanský, Robert; Brndiar, Ján; Li, Yan Jun; Štich, Ivan; Sugawara, Yasuhiro

2017-07-01

Probing physical quantities on the nanoscale that have directionality, such as magnetic moments, electric dipoles, or the force response of a surface, is essential for characterizing functionalized materials for nanotechnological device applications. Currently, such physical quantities are usually experimentally obtained as scalars. To investigate the physical properties of a surface on the nanoscale in depth, these properties must be measured as vectors. Here we demonstrate a three-force-component detection method, based on multi-frequency atomic force microscopy on the subatomic scale and apply it to a Ge(001)-c(4 × 2) surface. We probed the surface-normal and surface-parallel force components above the surface and their direction-dependent anisotropy and expressed them as a three-dimensional force vector distribution. Access to the atomic-scale force distribution on the surface will enable better understanding of nanoscale surface morphologies, chemical composition and reactions, probing nanostructures via atomic or molecular manipulation, and provide insights into the behaviour of nano-machines on substrates.

Accuracy and precision of loadsol® insole force-sensors for the quantification of ground reaction force-based biomechanical running parameters.

PubMed

Seiberl, Wolfgang; Jensen, Elisabeth; Merker, Josephine; Leitel, Marco; Schwirtz, Ansgar

2018-05-29

Force plates represent the "gold standard" in measuring running kinetics to predict performance or to identify the sources of running-related injuries. As these measurements are generally limited to laboratory analyses, wireless high-quality sensors for measuring in the field are needed. This work analysed the accuracy and precision of a new wireless insole forcesensor for quantifying running-related kinetic parameters. Vertical ground reaction force (GRF) was simultaneously measured with pit-mounted force plates (1 kHz) and loadsol ® sensors (100 Hz) under unshod forefoot and rearfoot running-step conditions. GRF data collections were repeated four times, each separated by 30 min treadmill running, to test influence of extended use. A repeated-measures ANOVA was used to identify differences between measurement devices. Additionally, mean bias and Bland-Altman limits of agreement (LoA) were calculated. We found a significant difference (p < .05) in ground contact time, peak force, and force rate, while there was no difference in parameters impulse, time to peak, and negative force rate. There was no influence of time point of measurement. The mean bias of ground contact time, impulse, peak force, and time to peak ranged between 0.6% and 3.4%, demonstrating high accuracy of loadsol ® devices for these parameters. For these same parameters, the LoA analysis showed that 95% of all measurement differences between insole and force plate measurements were less than 12%, demonstrating high precision of the sensors. However, highly dynamic behaviour of GRF, such as force rate, is not yet sufficiently resolved by the insole devices, which is likely explained by the low sampling rate.

DEMONSTRATION OF PILOT-SCALE PREVAPORATION SYSTEMS FOR VOLATILE ORGANIC COMPOUND REMOVAL FROM A SURFACTANT ENHANCED AQUIFER REMEDIATION FLUID. I. SPIRAL WOUND MEMBRANE MODULES

EPA Science Inventory

During the summer of 1996, a pilot-scale demonstration of a surfactant enhanced aquifer remediation (SEAR) process for removal of dense non-aqueous phase liquids (DNAPLs) from soils was conducted at Hill Air Force Base in Layton, Utah. Five thousand gallons of the extracted DNAP...

Defense Science Board Task Force Report on Next-Generation Unmanned Undersea Systems

DTIC Science & Technology

2016-10-01

active learning occurs in an environment that extends beyondchoreographed demonstrations designed to validate pre -determined hypotheses. Finally, when...4 OPNAV N99 should coordinate a broad-based design , development, and experimental effort to bypass traditional limitations for unmanned undersea...approaches that could facilitate rapid experimentation , operational demonstration of capabilities, and deployment of initial capabilities that show

X-48C Hybrid - Blended Wing Body Demonstrator

NASA Image and Video Library

2013-02-28

The NASA-Boeing X-48C Hybrid/Blended Wing Body research aircraft banked left during one of its final test flights over Edwards Air Force Base from NASA's Dryden Flight Research Center on Feb. 28, 2013.

Force-detected nanoscale absorption spectroscopy in water at room temperature using an optical trap

NASA Astrophysics Data System (ADS)

Parobek, Alexander; Black, Jacob W.; Kamenetska, Maria; Ganim, Ziad

2018-04-01

Measuring absorption spectra of single molecules presents a fundamental challenge for standard transmission-based instruments because of the inherently low signal relative to the large background of the excitation source. Here we demonstrate a new approach for performing absorption spectroscopy in solution using a force measurement to read out optical excitation at the nanoscale. The photoinduced force between model chromophores and an optically trapped gold nanoshell has been measured in water at room temperature. This photoinduced force is characterized as a function of wavelength to yield the force spectrum, which is shown to be correlated to the absorption spectrum for four model systems. The instrument constructed for these measurements combines an optical tweezer with frequency domain absorption spectroscopy over the 400-800 nm range. These measurements provide proof-of-principle experiments for force-detected nanoscale spectroscopies that operate under ambient chemical conditions.

Spin-orbit induced electronic spin separation in semiconductor nanostructures.

PubMed

Kohda, Makoto; Nakamura, Shuji; Nishihara, Yoshitaka; Kobayashi, Kensuke; Ono, Teruo; Ohe, Jun-ichiro; Tokura, Yasuhiro; Mineno, Taiki; Nitta, Junsaku

2012-01-01

The demonstration of quantized spin splitting by Stern and Gerlach is one of the most important experiments in modern physics. Their discovery was the precursor of recent developments in spin-based technologies. Although electrical spin separation of charged particles is fundamental in spintronics, in non-uniform magnetic fields it has been difficult to separate the spin states of charged particles due to the Lorentz force, as well as to the insufficient and uncontrollable field gradients. Here we demonstrate electronic spin separation in a semiconductor nanostructure. To avoid the Lorentz force, which is inevitably induced when an external magnetic field is applied, we utilized the effective non-uniform magnetic field which originates from the Rashba spin-orbit interaction in an InGaAs-based heterostructure. Using a Stern-Gerlach-inspired mechanism, together with a quantum point contact, we obtained field gradients of 10(8) T m(-1) resulting in a highly polarized spin current.

Spin–orbit induced electronic spin separation in semiconductor nanostructures

PubMed Central

Kohda, Makoto; Nakamura, Shuji; Nishihara, Yoshitaka; Kobayashi, Kensuke; Ono, Teruo; Ohe, Jun-ichiro; Tokura, Yasuhiro; Mineno, Taiki; Nitta, Junsaku

2012-01-01

The demonstration of quantized spin splitting by Stern and Gerlach is one of the most important experiments in modern physics. Their discovery was the precursor of recent developments in spin-based technologies. Although electrical spin separation of charged particles is fundamental in spintronics, in non-uniform magnetic fields it has been difficult to separate the spin states of charged particles due to the Lorentz force, as well as to the insufficient and uncontrollable field gradients. Here we demonstrate electronic spin separation in a semiconductor nanostructure. To avoid the Lorentz force, which is inevitably induced when an external magnetic field is applied, we utilized the effective non-uniform magnetic field which originates from the Rashba spin–orbit interaction in an InGaAs-based heterostructure. Using a Stern–Gerlach-inspired mechanism, together with a quantum point contact, we obtained field gradients of 108 T m−1 resulting in a highly polarized spin current. PMID:23011136

Elastic modulus measurements at variable temperature: Validation of atomic force microscopy techniques

NASA Astrophysics Data System (ADS)

Natali, Marco; Reggente, Melania; Passeri, Daniele; Rossi, Marco

2016-06-01

The development of polymer-based nanocomposites to be used in critical thermal environments requires the characterization of their mechanical properties, which are related to their chemical composition, size, morphology and operating temperature. Atomic force microscopy (AFM) has been proven to be a useful tool to develop techniques for the mechanical characterization of these materials, thanks to its nanometer lateral resolution and to the capability of exerting ultra-low loads, down to the piconewton range. In this work, we demonstrate two techniques, one quasi-static, i.e., AFM-based indentation (I-AFM), and one dynamic, i.e., contact resonance AFM (CR-AFM), for the mechanical characterization of compliant materials at variable temperature. A cross-validation of I-AFM and CR-AFM has been performed by comparing the results obtained on two reference materials, i.e., low-density polyethylene (LDPE) and polycarbonate (PC), which demonstrated the accuracy of the techniques.

The chocolate-colored expanse of Rogers Dry Lake frames the sleek lines of the Boeing / NASA X-48B subscale demonstrator during a test flight at Edwards AFB

NASA Image and Video Library

2007-08-14

Boeing Phantom Works' subscale Blended Wing Body technology demonstration aircraft began its initial flight tests from NASA's Dryden Flight Research Center at Edwards Air Force Base, Calif. in the summer of 2007. The 8.5 percent dynamically scaled unmanned aircraft, designated the X-48B by the Air Force, is designed to mimic the aerodynamic characteristics of a full-scale large cargo transport aircraft with the same blended wing body shape. The initial flight tests focused on evaluation of the X-48B's low-speed flight characteristics and handling qualities. About 25 flights were planned to gather data in these low-speed flight regimes. Based on the results of the initial flight test series, a second set of flight tests was planned to test the aircraft's low-noise and handling characteristics at transonic speeds.

KSC-06pd0188

NASA Image and Video Library

2006-01-18

VANDENBERG AIR FORCE BASE, Calif. — Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, the wrapped Space Technology 5 (ST5) spacecraft is ready for mating to the Pegasus XL launch vehicle. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0187

NASA Image and Video Library

2006-01-18

VANDENBERG AIR FORCE BASE, Calif. — Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, the wrapped Space Technology 5 (ST5) spacecraft is being prepared for mating to the Pegasus XL launch vehicle. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

Integrating viscoelastic mass spring dampers into position-based dynamics to simulate soft tissue deformation in real time

PubMed Central

Lu, Yuhua; Liu, Qian

2018-01-01

We propose a novel method to simulate soft tissue deformation for virtual surgery applications. The method considers the mechanical properties of soft tissue, such as its viscoelasticity, nonlinearity and incompressibility; its speed, stability and accuracy also meet the requirements for a surgery simulator. Modifying the traditional equation for mass spring dampers (MSD) introduces nonlinearity and viscoelasticity into the calculation of elastic force. Then, the elastic force is used in the constraint projection step for naturally reducing constraint potential. The node position is enforced by the combined spring force and constraint conservative force through Newton's second law. We conduct a comparison study of conventional MSD and position-based dynamics for our new integrating method. Our approach enables stable, fast and large step simulation by freely controlling visual effects based on nonlinearity, viscoelasticity and incompressibility. We implement a laparoscopic cholecystectomy simulator to demonstrate the practicality of our method, in which liver and gallbladder deformation can be simulated in real time. Our method is an appropriate choice for the development of real-time virtual surgery applications. PMID:29515870

Integrating viscoelastic mass spring dampers into position-based dynamics to simulate soft tissue deformation in real time.

PubMed

Xu, Lang; Lu, Yuhua; Liu, Qian

2018-02-01

We propose a novel method to simulate soft tissue deformation for virtual surgery applications. The method considers the mechanical properties of soft tissue, such as its viscoelasticity, nonlinearity and incompressibility; its speed, stability and accuracy also meet the requirements for a surgery simulator. Modifying the traditional equation for mass spring dampers (MSD) introduces nonlinearity and viscoelasticity into the calculation of elastic force. Then, the elastic force is used in the constraint projection step for naturally reducing constraint potential. The node position is enforced by the combined spring force and constraint conservative force through Newton's second law. We conduct a comparison study of conventional MSD and position-based dynamics for our new integrating method. Our approach enables stable, fast and large step simulation by freely controlling visual effects based on nonlinearity, viscoelasticity and incompressibility. We implement a laparoscopic cholecystectomy simulator to demonstrate the practicality of our method, in which liver and gallbladder deformation can be simulated in real time. Our method is an appropriate choice for the development of real-time virtual surgery applications.

Going Vertical To Improve the Accuracy of Atomic Force Microscopy Based Single-Molecule Force Spectroscopy.

PubMed

Walder, Robert; Van Patten, William J; Adhikari, Ayush; Perkins, Thomas T

2018-01-23

Single-molecule force spectroscopy (SMFS) is a powerful technique to characterize the energy landscape of individual proteins, the mechanical properties of nucleic acids, and the strength of receptor-ligand interactions. Atomic force microscopy (AFM)-based SMFS benefits from ongoing progress in improving the precision and stability of cantilevers and the AFM itself. Underappreciated is that the accuracy of such AFM studies remains hindered by inadvertently stretching molecules at an angle while measuring only the vertical component of the force and extension, degrading both measurements. This inaccuracy is particularly problematic in AFM studies using double-stranded DNA and RNA due to their large persistence length (p ≈ 50 nm), often limiting such studies to other SMFS platforms (e.g., custom-built optical and magnetic tweezers). Here, we developed an automated algorithm that aligns the AFM tip above the DNA's attachment point to a coverslip. Importantly, this algorithm was performed at low force (10-20 pN) and relatively fast (15-25 s), preserving the connection between the tip and the target molecule. Our data revealed large uncorrected lateral offsets for 100 and 650 nm DNA molecules [24 ± 18 nm (mean ± standard deviation) and 180 ± 110 nm, respectively]. Correcting this offset yielded a 3-fold improvement in accuracy and precision when characterizing DNA's overstretching transition. We also demonstrated high throughput by acquiring 88 geometrically corrected force-extension curves of a single individual 100 nm DNA molecule in ∼40 min and versatility by aligning polyprotein- and PEG-based protein-ligand assays. Importantly, our software-based algorithm was implemented on a commercial AFM, so it can be broadly adopted. More generally, this work illustrates how to enhance AFM-based SMFS by developing more sophisticated data-acquisition protocols.

DEMONSTRATION OF THE ENVIRONMENTAL AND DEMAND-SIDE MANAGEMENT BENEFITS OF GRID-CONNECTED PHOTOVOLTAIC POWER SYSTEMS SITED ON MILITARY BASES

EPA Science Inventory

The report gives results of an investigation into the pollutant emission reduction and demand-side management potential of three photovoltaic (PV) systems installed at Ft. Huachuca, AZ, Ft. Dix, NJ, and Hickam Air Force Base, HI, which began operation between January and July 199...

MATCH: An Atom- Typing Toolset for Molecular Mechanics Force Fields

PubMed Central

Yesselman, Joseph D.; Price, Daniel J.; Knight, Jennifer L.; Brooks, Charles L.

2011-01-01

We introduce a toolset of program libraries collectively titled MATCH (Multipurpose Atom-Typer for CHARMM) for the automated assignment of atom types and force field parameters for molecular mechanics simulation of organic molecules. The toolset includes utilities for the conversion from multiple chemical structure file formats into a molecular graph. A general chemical pattern-matching engine using this graph has been implemented whereby assignment of molecular mechanics atom types, charges and force field parameters is achieved by comparison against a customizable list of chemical fragments. While initially designed to complement the CHARMM simulation package and force fields by generating the necessary input topology and atom-type data files, MATCH can be expanded to any force field and program, and has core functionality that makes it extendable to other applications such as fragment-based property prediction. In the present work, we demonstrate the accurate construction of atomic parameters of molecules within each force field included in CHARMM36 through exhaustive cross validation studies illustrating that bond increment rules derived from one force field can be transferred to another. In addition, using leave-one-out substitution it is shown that it is also possible to substitute missing intra and intermolecular parameters with ones included in a force field to complete the parameterization of novel molecules. Finally, to demonstrate the robustness of MATCH and the coverage of chemical space offered by the recent CHARMM CGENFF force field (Vanommeslaeghe, et al., JCC., 2010, 31, 671–690), one million molecules from the PubChem database of small molecules are typed, parameterized and minimized. PMID:22042689

Integral force feedback control with input shaping: Application to piezo-based scanning systems in ECDLs.

PubMed

Zhang, Meng; Liu, Zhigang; Zhu, Yu; Bu, Mingfan; Hong, Jun

2017-07-01

In this paper, a hybrid control system is developed by integrating the closed-loop force feedback and input shaping method to overcome the problem of the hysteresis and dynamic behavior in piezo-based scanning systems and increase the scanning speed of tunable external cavity diode lasers. The flexible hinge and piezoelectric actuators are analyzed, and a dynamic model of the scanning systems is established. A force sensor and an integral controller are utilized in integral force feedback (IFF) to directly augment the damping of the piezoelectric scanning systems. Hysteresis has been effectively eliminated, but the mechanical resonance is still evident. Noticeable residual vibration occurred after the inflection points and then gradually disappeared. For the further control of mechanical resonance, based on the theory of minimum-acceleration trajectory planning, the time-domain input shaping method was developed. The turning sections of a scanning trajectory are replaced by smooth curves, while the linear sections are retained. The IFF method is combined with the input shaping method to control the non-linearity and mechanical resonance in high-speed piezo-based scanning systems. Experiments are conducted, and the results demonstrate the effectiveness of the proposed control approach.

Integral force feedback control with input shaping: Application to piezo-based scanning systems in ECDLs

NASA Astrophysics Data System (ADS)

Zhang, Meng; Liu, Zhigang; Zhu, Yu; Bu, Mingfan; Hong, Jun

2017-07-01

In this paper, a hybrid control system is developed by integrating the closed-loop force feedback and input shaping method to overcome the problem of the hysteresis and dynamic behavior in piezo-based scanning systems and increase the scanning speed of tunable external cavity diode lasers. The flexible hinge and piezoelectric actuators are analyzed, and a dynamic model of the scanning systems is established. A force sensor and an integral controller are utilized in integral force feedback (IFF) to directly augment the damping of the piezoelectric scanning systems. Hysteresis has been effectively eliminated, but the mechanical resonance is still evident. Noticeable residual vibration occurred after the inflection points and then gradually disappeared. For the further control of mechanical resonance, based on the theory of minimum-acceleration trajectory planning, the time-domain input shaping method was developed. The turning sections of a scanning trajectory are replaced by smooth curves, while the linear sections are retained. The IFF method is combined with the input shaping method to control the non-linearity and mechanical resonance in high-speed piezo-based scanning systems. Experiments are conducted, and the results demonstrate the effectiveness of the proposed control approach.

Gas-Liquid Separation Strategies in Microgravity Environment

NASA Technical Reports Server (NTRS)

Antar, Basil N.; Reiss, Donald A.; Lehman, Daniel

2006-01-01

Bubble entrainment in liquids represents a serious problem in the microgravity environment. Whenever bubbles are entrained in a liquid,they tend to remain stationary in the liquid bulk in the absence of any external forcing. This is due to the reduction or complete absence of the buoyancy force in the microgravity environment, Thus the buoyancy force can not the be exploited to place the bubbles at the top of the liquid volume as in Ig(sub o) conditions. This situation represents a serious drawback in many space based engineering and scientific applications. We have demonstrated in a series of low gravity experiments conducted during parabolic flight on board aircraft that bubbles can be controlled in such a manner as to increase,the probability of their expulsion from a liquid bulk. In these tests the liquid'bulk was made either to be contained within, or to flow through specially designed containers using capillary force alone. Such containers appear to facilitate bubble removal, from the liquid bulk. Different successful liquid flow configurations will be discussed and the efficacy of the resulting bubble expulsion mechanisms will be demonstrated.

A pressure-based force and torque prediction technique for the study of fish-like swimming

PubMed Central

Dabiri, John O.; Lauder, George V.

2017-01-01

Many outstanding questions about the evolution and function of fish morphology are linked to swimming dynamics, and a detailed knowledge of time-varying forces and torques along the animal’s body is a key component in answering many of these questions. Yet, quantifying these forces and torques experimentally represents a major challenge that to date prevents a full understanding of fish-like swimming. Here, we develop a method for obtaining these force and torque data non-invasively using standard 2D digital particle image velocimetry in conjunction with a pressure field algorithm. We use a mechanical flapping foil apparatus to model fish-like swimming and measure forces and torques directly with a load cell, and compare these measured values to those estimated simultaneously using our pressure-based approach. We demonstrate that, when out-of-plane flows are relatively small compared to the planar flow, and when pressure effects sufficiently dominate shear effects, this technique is able to accurately reproduce the shape, magnitude, and timing of locomotor forces and torques experienced by a fish-like swimmer. We conclude by exploring of the limits of this approach and its feasibility in the study of freely-swimming fishes. PMID:29216264

An Atomic Force Microscope with Dual Actuation Capability for Biomolecular Experiments

NASA Astrophysics Data System (ADS)

Sevim, Semih; Shamsudhin, Naveen; Ozer, Sevil; Feng, Luying; Fakhraee, Arielle; Ergeneman, Olgaç; Pané, Salvador; Nelson, Bradley J.; Torun, Hamdi

2016-06-01

We report a modular atomic force microscope (AFM) design for biomolecular experiments. The AFM head uses readily available components and incorporates deflection-based optics and a piezotube-based cantilever actuator. Jetted-polymers have been used in the mechanical assembly, which allows rapid manufacturing. In addition, a FeCo-tipped electromagnet provides high-force cantilever actuation with vertical magnetic fields up to 0.55 T. Magnetic field calibration has been performed with a micro-hall sensor, which corresponds well with results from finite element magnetostatics simulations. An integrated force resolution of 1.82 and 2.98 pN, in air and in DI water, respectively was achieved in 1 kHz bandwidth with commercially available cantilevers made of Silicon Nitride. The controller and user interface are implemented on modular hardware to ensure scalability. The AFM can be operated in different modes, such as molecular pulling or force-clamp, by actuating the cantilever with the available actuators. The electromagnetic and piezoelectric actuation capabilities have been demonstrated in unbinding experiments of the biotin-streptavidin complex.

Optimal design of high damping force engine mount featuring MR valve structure with both annular and radial flow paths

NASA Astrophysics Data System (ADS)

Nguyen, Q. H.; Choi, S. B.; Lee, Y. S.; Han, M. S.

2013-11-01

This paper focuses on the optimal design of a compact and high damping force engine mount featuring magnetorheological fluid (MRF). In the mount, a MR valve structure with both annular and radial flows is employed to generate a high damping force. First, the configuration and working principle of the proposed MR mount is introduced. The MRF flows in the mount are then analyzed and the governing equations of the MR mount are derived based on the Bingham plastic behavior of the MRF. An optimal design of the MR mount is then performed to find the optimal structure of the MR valve to generate a maximum damping force with certain design constraints. In addition, the gap size of MRF ducts is empirically chosen considering the ‘lockup’ problem of the mount at high frequency. Performance of the optimized MR mount is then evaluated based on finite element analysis and discussions on performance results of the optimized MR mount are given. The effectiveness of the proposed MR engine mount is demonstrated via computer simulation by presenting damping force and power consumption.

An Atomic Force Microscope with Dual Actuation Capability for Biomolecular Experiments

PubMed Central

Sevim, Semih; Shamsudhin, Naveen; Ozer, Sevil; Feng, Luying; Fakhraee, Arielle; Ergeneman, Olgaç; Pané, Salvador; Nelson, Bradley J.; Torun, Hamdi

2016-01-01

We report a modular atomic force microscope (AFM) design for biomolecular experiments. The AFM head uses readily available components and incorporates deflection-based optics and a piezotube-based cantilever actuator. Jetted-polymers have been used in the mechanical assembly, which allows rapid manufacturing. In addition, a FeCo-tipped electromagnet provides high-force cantilever actuation with vertical magnetic fields up to 0.55 T. Magnetic field calibration has been performed with a micro-hall sensor, which corresponds well with results from finite element magnetostatics simulations. An integrated force resolution of 1.82 and 2.98 pN, in air and in DI water, respectively was achieved in 1 kHz bandwidth with commercially available cantilevers made of Silicon Nitride. The controller and user interface are implemented on modular hardware to ensure scalability. The AFM can be operated in different modes, such as molecular pulling or force-clamp, by actuating the cantilever with the available actuators. The electromagnetic and piezoelectric actuation capabilities have been demonstrated in unbinding experiments of the biotin-streptavidin complex. PMID:27273214

Specialized probes based on hydroxyapatite calcium for heart tissues research by atomic force microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhukov, Mikhail, E-mail: cloudjyk@yandex.ru; Golubok, Alexander; Institute for Analytical Instrumentation, Russian Academy of Sciences

The new specialized AFM-probes with hydroxyapatite structures for atomic force microscopy of heart tissues calcification were created and studied. A process of probe fabrication is demonstrated. The adhesive forces between specialized hydroxyapatite probe and endothelium/subendothelial layers were investigated. It was found that the adhesion forces are significantly higher for the subendothelial layers. We consider that it is connected with the formation and localization of hydroxyapatite in the area of subendothelial layers of heart tissues. In addition, the roughness analysis and structure visualization of the endothelial surface of the heart tissue were carried out. The results show high efficiency of createdmore » specialized probes at study a calcinations process of the aortic heart tissues.« less

Networking the Global Maritime Partnership

DTIC Science & Technology

2016-04-13

Strike, and Sea Basing) as “old wine in new bottles,” but with them Admiral Clark introduced a new term, “FORCEnet,” which referred to “an initiative...the primary reasons for the overwhelming combat power demonstrated in recent operations.18 C4ISR advances not only benefit so-called high-end...of the benefits of the adoption by coalition naval forces of a networked force structure. The report of AG-1 prompted leaders of the MAR in 2005 to

Combination of atomic force microscopy and mass spectrometry for the detection of target protein in the serum samples of children with autism spectrum disorders

NASA Astrophysics Data System (ADS)

Kaysheva, A. L.; Pleshakova, T. O.; Kopylov, A. T.; Shumov, I. D.; Iourov, I. Y.; Vorsanova, S. G.; Yurov, Y. B.; Ziborov, V. S.; Archakov, A. I.; Ivanov, Y. D.

2017-10-01

Possibility of detection of target proteins associated with development of autistic disorders in children with use of combined atomic force microscopy and mass spectrometry (AFM/MS) method is demonstrated. The proposed method is based on the combination of affine enrichment of proteins from biological samples and visualization of these proteins by AFM and MS analysis with quantitative detection of target proteins.

Direct measurement of optical trapping force gradient on polystyrene microspheres using a carbon nanotube mechanical resonator.

PubMed

Yasuda, Masaaki; Takei, Kuniharu; Arie, Takayuki; Akita, Seiji

2017-06-06

Optical tweezers based on optical radiation pressure are widely used to manipulate nanoscale to microscale particles. This study demonstrates direct measurement of the optical force gradient distribution acting on a polystyrene (PS) microsphere using a carbon nanotube (CNT) mechanical resonator, where a PS microsphere with 3 μm diameter is welded at the CNT tip using laser heating. With the CNT mechanical resonator with PS microsphere, we measured the distribution of optical force gradient with resolution near the thermal noise limit of 0.02 pN/μm in vacuum, in which condition enables us to high accuracy measurement using the CNT mechanical resonator because of reduced mechanical damping from surrounding fluid. The obtained force gradient and the force gradient distribution agree well with theoretical values calculated using Lorenz-Mie theory.

KSC-07pd3080

NASA Image and Video Library

2007-11-02

KENNEDY SPACE CENTER, FLA. -- During a rehearsal for the World Space Expo Aerial Salute at NASA's Kennedy Space Center, an honor guard from Patrick Air Force Base marches along the NASA Causeway. Commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond, the expo will showcase various panels, presentations and educational programs, as well as the air show. Participating in the air show are the U.S. Air Force Thunderbirds, U.S. Air Force F-22 Raptor, U.S. Navy F-18 Super Hornet, U.S. Air Force F-15 Eagle, the P-51 Mustang Heritage Flight, and the U.S. Air Force 920th Rescue Wing, which was responsible for Mercury and Gemini capsule recovery. The U.S. Army Golden Knights will also demonstrate precision skydiving. Photo credit: NASA/George Shelton.

Comparative analysis of internal friction and natural frequency measured by free decay and forced vibration.

PubMed

Wang, Y Z; Ding, X D; Xiong, X M; Zhang, J X

2007-10-01

Relations between various values of the internal friction (tgdelta, Q(-1), Q(-1*), and Lambda/pi) measured by free decay and forced vibration are analyzed systemically based on a fundamental mechanical model in this paper. Additionally, relations between various natural frequencies, such as vibration frequency of free decay omega(FD), displacement-resonant frequency of forced vibration omega(d), and velocity-resonant frequency of forced vibration omega(0) are calculated. Moreover, measurement of natural frequencies of a copper specimen of 99.9% purity has been made to demonstrate the relation between the measured natural frequencies of the system by forced vibration and free decay. These results are of importance for not only more accurate measurement of the elastic modulus of materials but also the data conversion between different internal friction measurements.

Interaction force and motion estimators facilitating impedance control of the upper limb rehabilitation robot.

PubMed

Mancisidor, Aitziber; Zubizarreta, Asier; Cabanes, Itziar; Bengoa, Pablo; Jung, Je Hyung

2017-07-01

In order to enhance the performance of rehabilitation robots, it is imperative to know both force and motion caused by the interaction between user and robot. However, common direct measurement of both signals through force and motion sensors not only increases the complexity of the system but also impedes affordability of the system. As an alternative of the direct measurement, in this work, we present new force and motion estimators for the proper control of the upper-limb rehabilitation Universal Haptic Pantograph (UHP) robot. The estimators are based on the kinematic and dynamic model of the UHP and the use of signals measured by means of common low-cost sensors. In order to demonstrate the effectiveness of the estimators, several experimental tests were carried out. The force and impedance control of the UHP was implemented first by directly measuring the interaction force using accurate extra sensors and the robot performance was compared to the case where the proposed estimators replace the direct measured values. The experimental results reveal that the controller based on the estimators has similar performance to that using direct measurement (less than 1 N difference in root mean square error between two cases), indicating that the proposed force and motion estimators can facilitate implementation of interactive controller for the UHP in robotmediated rehabilitation trainings.

Finding the Cell Center by a Balance of Dynein and Myosin Pulling and Microtubule Pushing: A Computational Study

PubMed Central

Zhu, Jie; Burakov, Anton; Rodionov, Vladimir

2010-01-01

The centrosome position in many types of interphase cells is actively maintained in the cell center. Our previous work indicated that the centrosome is kept at the center by pulling force generated by dynein and actin flow produced by myosin contraction and that an unidentified factor that depends on microtubule dynamics destabilizes position of the centrosome. Here, we use modeling to simulate the centrosome positioning based on the idea that the balance of three forces—dyneins pulling along microtubule length, myosin-powered centripetal drag, and microtubules pushing on organelles—is responsible for the centrosome displacement. By comparing numerical predictions with centrosome behavior in wild-type and perturbed interphase cells, we rule out several plausible hypotheses about the nature of the microtubule-based force. We conclude that strong dynein- and weaker myosin-generated forces pull the microtubules inward competing with microtubule plus-ends pushing the microtubule aster outward and that the balance of these forces positions the centrosome at the cell center. The model also predicts that kinesin action could be another outward-pushing force. Simulations demonstrate that the force-balance centering mechanism is robust yet versatile. We use the experimental observations to reverse engineer the characteristic forces and centrosome mobility. PMID:20980619

A Systematic Approach to Predicting Spring Force for Sagittal Craniosynostosis Surgery.

PubMed

Zhang, Guangming; Tan, Hua; Qian, Xiaohua; Zhang, Jian; Li, King; David, Lisa R; Zhou, Xiaobo

2016-05-01

Spring-assisted surgery (SAS) can effectively treat scaphocephaly by reshaping crania with the appropriate spring force. However, it is difficult to accurately estimate spring force without considering biomechanical properties of tissues. This study presents and validates a reliable system to accurately predict the spring force for sagittal craniosynostosis surgery. The authors randomly chose 23 patients who underwent SAS and had been followed for at least 2 years. An elastic model was designed to characterize the biomechanical behavior of calvarial bone tissue for each individual. After simulating the contact force on accurate position of the skull strip with the springs, the finite element method was applied to calculating the stress of each tissue node based on the elastic model. A support vector regression approach was then used to model the relationships between biomechanical properties generated from spring force, bone thickness, and the change of cephalic index after surgery. Therefore, for a new patient, the optimal spring force can be predicted based on the learned model with virtual spring simulation and dynamic programming approach prior to SAS. Leave-one-out cross-validation was implemented to assess the accuracy of our prediction. As a result, the mean prediction accuracy of this model was 93.35%, demonstrating the great potential of this model as a useful adjunct for preoperative planning tool.

MEMS-based Force-clamp Analysis of the Role of Body Stiffness in C. elegans Touch Sensation

PubMed Central

Petzold, Bryan C.; Park, Sung-Jin; Mazzochette, Eileen A.; Goodman, Miriam B.; Pruitt, Beth L.

2013-01-01

Touch is enabled by mechanoreceptor neurons in the skin and plays an essential role in our everyday lives, but is among the least understood of our five basic senses. Force applied to the skin deforms these neurons and activates ion channels within them. Despite the importance of the mechanics of the skin in determining mechanoreceptor neuron deformation and ultimately touch sensation, the role of mechanics in touch sensitivity is poorly understood. Here, we use the model organism Caenorhabditis elegans to directly test the hypothesis that body mechanics modulate touch sensitivity. We demonstrate a microelectromechanical system (MEMS)-based force clamp that can apply calibrated forces to freely crawling C. elegans worms and measure touch-evoked avoidance responses. This approach reveals that wild-type animals sense forces < 1 μN and indentation depths < 1 μm. We use both genetic manipulation of the skin and optogenetic modulation of body wall muscles to alter body mechanics. We find that small changes in body stiffness dramatically affect force sensitivity, while having only modest effects on indentation sensitivity. We investigate the theoretical body deformation predicted under applied force and conclude that local mechanical loads induce inward bending deformation of the skin to drive touch sensation in C. elegans. PMID:23598612

Computational studies of steering nanoparticles with magnetic gradients

NASA Astrophysics Data System (ADS)

Aylak, Sultan Suleyman

Magnetic Resonance Imaging (MRI) guided nanorobotic systems that could perform diagnostic, curative, and reconstructive treatments in the human body at the cellular and subcellular level in a controllable manner have recently been proposed. The concept of a MRI-guided nanorobotic system is based on the use of a MRI scanner to induce the required external driving forces to guide magnetic nanocapsules to a specific target. However, the maximum magnetic gradient specifications of existing clinical MRI systems are not capable of driving magnetic nanocapsules against the blood flow. This thesis presents the visualization of nanoparticles inside blood vessel, Graphical User Interface (GUI) for updating file including initial parameters and demonstrating the simulation of particles and C++ code for computing magnetic forces and fluidic forces. The visualization and GUI were designed using Virtual Reality Modeling Language (VRML), MATLAB and C#. The addition of software for MRI-guided nanorobotic system provides simulation results. Preliminary simulation results demonstrate that external magnetic field causes aggregation of nanoparticles while they flow in the vessel. This is a promising result --in accordance with similar experimental results- and encourages further investigation on the nanoparticle-based self-assembly structures for use in nanorobotic drug delivery.

Enantiospecific Detection of Chiral Nanosamples Using Photoinduced Force

NASA Astrophysics Data System (ADS)

Kamandi, Mohammad; Albooyeh, Mohammad; Guclu, Caner; Veysi, Mehdi; Zeng, Jinwei; Wickramasinghe, Kumar; Capolino, Filippo

2017-12-01

We propose a high-resolution microscopy technique for enantiospecific detection of chiral samples down to sub-100-nm size based on force measurement. We delve into the differential photoinduced optical force Δ F exerted on an achiral probe in the vicinity of a chiral sample when left and right circularly polarized beams separately excite the sample-probe interactive system. We analytically prove that Δ F is entangled with the enantiomer type of the sample enabling enantiospecific detection of chiral inclusions. Moreover, we demonstrate that Δ F is linearly dependent on both the chiral response of the sample and the electric response of the tip and is inversely related to the quartic power of probe-sample distance. We provide physical insight into the transfer of optical activity from the chiral sample to the achiral tip based on a rigorous analytical approach. We support our theoretical achievements by several numerical examples highlighting the potential application of the derived analytic properties. Lastly, we demonstrate the sensitivity of our method to enantiospecify nanoscale chiral samples with chirality parameter on the order of 0.01 and discuss how the sensitivity of our proposed technique can be further improved.

Rigid two-axis MEMS force plate for measuring cellular traction force

NASA Astrophysics Data System (ADS)

Takahashi, Hidetoshi; Jung, Uijin G.; Kan, Tetsuo; Tsukagoshi, Takuya; Matsumoto, Kiyoshi; Shimoyama, Isao

2016-10-01

Cellular traction force is one of the important factors for understanding cell behaviors, such as spreading, migration and differentiation. Cells are known to change their behavior according to the mechanical stiffness of the environment. However, the measurement of cell traction forces on a rigid environment has remained difficult. This paper reports a micro-electromechanical systems (MEMS) force plate that provides a cellular traction force measurement on a rigid substrate. Both the high force sensitivity and high stiffness of the substrate were obtained using piezoresistive sensing elements. The proposed force plate consists of a 70 µm  ×  15 µm  ×  5 µm base as the substrate for cultivating a bovine aortic smooth muscle cell, and the supporting beams with piezoresistors on the sidewall and the surface were used to measure the forces in both the horizontal and vertical directions. The spring constant and force resolution of the fabricated force plate in the horizontal direction were 0.2 N m-1 and less than 0.05 µN, respectively. The cell traction force was measured, and the traction force increased by approximately 1 µN over 30 min. These results demonstrate that the proposed force plate is applicable as an effective traction force measurement.

Forces in General Relativity

ERIC Educational Resources Information Center

Ridgely, Charles T.

2010-01-01

Many textbooks dealing with general relativity do not demonstrate the derivation of forces in enough detail. The analyses presented herein demonstrate straightforward methods for computing forces by way of general relativity. Covariant divergence of the stress-energy-momentum tensor is used to derive a general expression of the force experienced…

A micro-scale cutting model for UD CFRP composites with thermo-mechanical coupling

DOE PAGES

Cheng, Hui; Gao, Jiaying; Kafka, Orion Landauer; ...

2017-09-23

Cutting a unidirectional carbon fiber-reinforced polymer (UD CFRP) structure is the basic unit for CFRP machining, which is a complex thermal-mechanically coupled process. To reveal the deformation mechanism and predict cutting force in UD CFRP micro cutting, a micro-scale fracture model for UD CFRP cutting with thermal-mechanical coupling is demonstrated in this paper, which captures the failure modes for fibers, matrix and the interface based on a micro-level RVE using a relatively simple damage based fracture method. The thermal-mechanical coupling model at the micro scale is developed on the basis of the plastic energy dissipation and frictional heating during cutting.more » Failure models for the fiber, matrix and interface region are applied depending on the material properties of each of these three phases. Numerical simulations based on the above model with different fiber orientations were performed to predict the deformation and forces of different components in UD CFRP. Cutting experiments with the same fiber orientations as considered in the simulations were carried out to validate the force and deformation results. The predicted force and deformation patterns match well with evidence from our experiments. In general, the cutting force is larger than the thrust force regardless of fiber orientation. The cutting force reaches a maximum as the fiber orientation approaches 90 , but thrust forces do not vary substantially across cases. When the fiber orientation is acute, the deformation of fibers is much smaller than when the cutting angle is obtuse. Surface roughness follows the same trend with cutting angle as fiber deformation.« less

A micro-scale cutting model for UD CFRP composites with thermo-mechanical coupling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cheng, Hui; Gao, Jiaying; Kafka, Orion Landauer

Cutting a unidirectional carbon fiber-reinforced polymer (UD CFRP) structure is the basic unit for CFRP machining, which is a complex thermal-mechanically coupled process. To reveal the deformation mechanism and predict cutting force in UD CFRP micro cutting, a micro-scale fracture model for UD CFRP cutting with thermal-mechanical coupling is demonstrated in this paper, which captures the failure modes for fibers, matrix and the interface based on a micro-level RVE using a relatively simple damage based fracture method. The thermal-mechanical coupling model at the micro scale is developed on the basis of the plastic energy dissipation and frictional heating during cutting.more » Failure models for the fiber, matrix and interface region are applied depending on the material properties of each of these three phases. Numerical simulations based on the above model with different fiber orientations were performed to predict the deformation and forces of different components in UD CFRP. Cutting experiments with the same fiber orientations as considered in the simulations were carried out to validate the force and deformation results. The predicted force and deformation patterns match well with evidence from our experiments. In general, the cutting force is larger than the thrust force regardless of fiber orientation. The cutting force reaches a maximum as the fiber orientation approaches 90 , but thrust forces do not vary substantially across cases. When the fiber orientation is acute, the deformation of fibers is much smaller than when the cutting angle is obtuse. Surface roughness follows the same trend with cutting angle as fiber deformation.« less

Force spectroscopy of quadruple H-bonded dimers by AFM: dynamic bond rupture and molecular time-temperature superposition.

PubMed

Zou, Shan; Schönherr, Holger; Vancso, G Julius

2005-08-17

We report on the application of the time-temperature superposition principle to supramolecular bond-rupture forces on the single-molecule level. The construction of force-loading rate master curves using atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) experiments carried out in situ at different temperatures allows one to extend the limited range of the experimentally accessible loading rates and hence to cross from thermodynamic nonequilibrium to quasi-equilibrium states. The approach is demonstrated for quadruple H-bonded ureido-4[1H]-pyrimidinone (UPy) moieties studied by variable-temperature SMFS in organic media. The unbinding forces of single quadruple H-bonding (UPy)2 complexes, which were identified based on a polymeric spacer strategy, were found to depend on the loading rate in the range of 5 nN/s to 500 nN/s at 301 K in hexadecane. By contrast, these rupture forces were independent of the loading rate from 5 to 200 nN/s at 330 K. These results indicate that the unbinding behavior of individual supramolecular complexes can be directly probed under both thermodynamic nonequilibrium and quasi-equilibrium conditions. On the basis of the time-temperature superposition principle, a master curve was constructed for a reference temperature of 301 K, and the crossover force (from loading-rate independent to -dependent regimes) was determined as approximately 145 pN (at a loading rate of approximately 5.6 nN/s). This approach significantly broadens the accessible loading-rate range and hence provides access to fine details of potential energy landscape of supramolecular complexes based on SMFS experiments.

KSC-08pd1348

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- A representative of the 301st Rescue Squadron and a volunteer (in the stretcher) from the NASA Vehicle Integration Test Team office get ready to demonstrate rescue equipment on the HH-60G helicopter that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base, Fla. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

KSC-08pd1347

NASA Image and Video Library

2008-05-12

CAPE CANAVERAL, Fla. -- A representative of the 301st Rescue Squadron and a volunteer from the NASA Vehicle Integration Test Team office get ready to demonstrate rescue equipment that is used by participants in the Mode VIII exercise being conducted at Patrick Air Force Base. In the background is an HH-60G helicopter. In support of, and with logistical support from, NASA, USSTRATCOM is hosting a major exercise involving Department of Defense, Department of Homeland Security, search and rescue (SAR) forces, including the 45th Space Wing at Patrick Air Force Base, which support space shuttle astronaut bailout contingency operations, known as Mode VIII. This exercise tests SAR capabilities to locate, recover and provide medical treatment for astronauts following a space shuttle launch phase open-ocean bailout. Participants include members of the U.S. Navy, U.S. Coast Guard, U.S. Air Force, and NASA's Kennedy Space Center and Johnson Space Center. This will be the 15th Mode VIII exercise conducted in the past 20 years. Photo credit: NASA/Kim Shiflett

Opto electronic tweezers based smart sweeper for cells/micro-particles sorting

NASA Astrophysics Data System (ADS)

Verma, R. S.; Kumar, N.

2018-04-01

We report on use of opto-electronic tweezers based sorting approach, termed as smart sweepers, for sorting the microscopic particles by using the Dielectrophoretic (DEP) force response of cells on applied a.c. bias frequency. The applied a.c. bias was kept in negative DEP region, close to the crossover frequency of one of the particles. A line shaped intensity pattern, generated by a cylindrical lens, was scanned across the mixture sample. The particles whose cross over frequency was close to the applied bias frequency, experienced negligible negative DEP(n-DEP) force. On the other hand, the other type of particle experienced large repelling force and were forced to move along the scanning direction of the line shaped intensity profile. We, as a proof of concept, demonstrated the working principle of opto electronic smart sweepers by sweeping out the polystyrene particles from a mixture consisting of polystyrene microspheres (PSM) and red blood cells (RBCs) and leaving RBCs in the region of interest.

KSC-04pd1257

NASA Image and Video Library

2004-06-09

KENNEDY SPACE CENTER, FLA. - At Cape Canaveral Air Force Station, Firefighter Chris Maupin (left) and Lt. Keith Abell demonstrate how the special aircraft firefighting vehicle (known as ARF) was used at the site of a recent fire in Brevard County, Fla. The firefighters sit inside the vehicle with a "driver" in the middle. They are able to direct the hoses to attack fires from above and below. The firefighters teamed up with task forces from Satellite Beach, Malabar, Melbourne, Palm Bay, Patrick Air Force Base and Brevard County to help fight wildfires in the Palm Bay and Malabar areas that threatened homes and property during the Memorial Day holiday weekend.

A New Version of an Old Demonstration Experiment Using the Elihu Thomson Jumping Ring Apparatus

ERIC Educational Resources Information Center

Foster, Theodore; Cary, Arthur; Mottmann, John; van Wyngaarden, Willem

2016-01-01

The goal of this paper is to make more widely known an eye-catching demonstration experiment in which a hanging conducting can is made to spin when placed near the iron core of an Elihu Thomson "jumping ring" apparatus. An explanation is given based on Faraday's law of induced voltages and the magnetic forces due to the core's fields…

R.E.D. Server: a web service for deriving RESP and ESP charges and building force field libraries for new molecules and molecular fragments.

PubMed

Vanquelef, Enguerran; Simon, Sabrina; Marquant, Gaelle; Garcia, Elodie; Klimerak, Geoffroy; Delepine, Jean Charles; Cieplak, Piotr; Dupradeau, François-Yves

2011-07-01

R.E.D. Server is a unique, open web service, designed to derive non-polarizable RESP and ESP charges and to build force field libraries for new molecules/molecular fragments. It provides to computational biologists the means to derive rigorously molecular electrostatic potential-based charges embedded in force field libraries that are ready to be used in force field development, charge validation and molecular dynamics simulations. R.E.D. Server interfaces quantum mechanics programs, the RESP program and the latest version of the R.E.D. tools. A two step approach has been developed. The first one consists of preparing P2N file(s) to rigorously define key elements such as atom names, topology and chemical equivalencing needed when building a force field library. Then, P2N files are used to derive RESP or ESP charges embedded in force field libraries in the Tripos mol2 format. In complex cases an entire set of force field libraries or force field topology database is generated. Other features developed in R.E.D. Server include help services, a demonstration, tutorials, frequently asked questions, Jmol-based tools useful to construct PDB input files and parse R.E.D. Server outputs as well as a graphical queuing system allowing any user to check the status of R.E.D. Server jobs.

DefenseLink.mil - Special Report - JCOC 76

Science.gov Websites

members from the 10th Special Forces Group demonstrate to the participants of the 76th Joint Civilian Orientation Conference receive a non-lethal force demonstration from U.S. Marine Staff Sgt. Deines in the 76th Joint Civilian Orientation Conference receive a non-lethal force demonstration from U.S

Digital force-feedback for protein unfolding experiments using atomic force microscopy

NASA Astrophysics Data System (ADS)

Bippes, Christian A.; Janovjak, Harald; Kedrov, Alexej; Muller, Daniel J.

2007-01-01

Since its invention in the 1990s single-molecule force spectroscopy has been increasingly applied to study protein (un-)folding, cell adhesion, and ligand-receptor interactions. In most force spectroscopy studies, the cantilever of an atomic force microscope (AFM) is separated from a surface at a constant velocity, thus applying an increasing force to folded bio-molecules or bio-molecular bonds. Recently, Fernandez and co-workers introduced the so-called force-clamp technique. Single proteins were subjected to a defined constant force allowing their life times and life time distributions to be directly measured. Up to now, the force-clamping was performed by analogue PID controllers, which require complex additional hardware and might make it difficult to combine the force-feedback with other modes such as constant velocity. These points may be limiting the applicability and versatility of this technique. Here we present a simple, fast, and all-digital (software-based) PID controller that yields response times of a few milliseconds in combination with a commercial AFM. We demonstrate the performance of our feedback loop by force-clamp unfolding of single Ig27 domains of titin and the membrane proteins bacteriorhodopsin (BR) and the sodium/proton antiporter NhaA.

Nanofabrication technique based on localized photocatalytic reactions using a TiO2-coated atomic force microscopy probe

NASA Astrophysics Data System (ADS)

Shibata, Takayuki; Iio, Naohiro; Furukawa, Hiromi; Nagai, Moeto

2017-02-01

We performed a fundamental study on the photocatalytic degradation of fluorescently labeled DNA molecules immobilized on titanium dioxide (TiO2) thin films under ultraviolet irradiation. The films were prepared by the electrochemical anodization of Ti thin films sputtered on silicon substrates. We also confirmed that the photocurrent arising from the photocatalytic oxidation of DNA molecules can be detected during this process. We then demonstrated an atomic force microscopy (AFM)-based nanofabrication technique by employing TiO2-coated AFM probes to penetrate living cell membranes under near-physiological conditions for minimally invasive intracellular delivery.

Estimation of elbow flexion force during isometric muscle contraction from mechanomyography and electromyography.

PubMed

Youn, Wonkeun; Kim, Jung

2010-11-01

Mechanomyography (MMG) is the muscle surface oscillations that are generated by the dimensional change of the contracting muscle fibers. Because MMG reflects the number of recruited motor units and their firing rates, just as electromyography (EMG) is influenced by these two factors, it can be used to estimate the force exerted by skeletal muscles. The aim of this study was to demonstrate the feasibility of MMG for estimating the elbow flexion force at the wrist under an isometric contraction by using an artificial neural network in comparison with EMG. We performed experiments with five subjects, and the force at the wrist and the MMG from the contributing muscles were recorded. It was found that MMG could be utilized to accurately estimate the isometric elbow flexion force based on the values of the normalized root mean square error (NRMSE = 0.131 ± 0.018) and the cross-correlation coefficient (CORR = 0.892 ± 0.033). Although MMG can be influenced by the physical milieu/morphology of the muscle and EMG performed better than MMG, these experimental results suggest that MMG has the potential to estimate muscle forces. These experimental results also demonstrated that MMG in combination with EMG resulted in better performance estimation in comparison with EMG or MMG alone, indicating that a combination of MMG and EMG signals could be used to provide complimentary information on muscle contraction.

Force-Induced Rupture of a DNA Duplex: From Fundamentals to Force Sensors.

PubMed

Mosayebi, Majid; Louis, Ard A; Doye, Jonathan P K; Ouldridge, Thomas E

2015-12-22

The rupture of double-stranded DNA under stress is a key process in biophysics and nanotechnology. In this article, we consider the shear-induced rupture of short DNA duplexes, a system that has been given new importance by recently designed force sensors and nanotechnological devices. We argue that rupture must be understood as an activated process, where the duplex state is metastable and the strands will separate in a finite time that depends on the duplex length and the force applied. Thus, the critical shearing force required to rupture a duplex depends strongly on the time scale of observation. We use simple models of DNA to show that this approach naturally captures the observed dependence of the force required to rupture a duplex within a given time on duplex length. In particular, this critical force is zero for the shortest duplexes, before rising sharply and then plateauing in the long length limit. The prevailing approach, based on identifying when the presence of each additional base pair within the duplex is thermodynamically unfavorable rather than allowing for metastability, does not predict a time-scale-dependent critical force and does not naturally incorporate a critical force of zero for the shortest duplexes. We demonstrate that our findings have important consequences for the behavior of a new force-sensing nanodevice, which operates in a mixed mode that interpolates between shearing and unzipping. At a fixed time scale and duplex length, the critical force exhibits a sigmoidal dependence on the fraction of the duplex that is subject to shearing.

3-DOF Force-Sensing Motorized Micro-Forceps for Robot-Assisted Vitreoretinal Surgery

PubMed Central

Gonenc, Berk; Chamani, Alireza; Handa, James; Gehlbach, Peter; Taylor, Russell H.; Iordachita, Iulian

2017-01-01

In vitreoretinal surgery, membrane peeling is a prototypical task where a layer of fibrous tissue is delaminated off the retina with a micro-forceps by applying very fine forces that are mostly imperceptible to the surgeon. Previously we developed sensitized ophthalmic surgery tools based on fiber Bragg grating (FBG) strain sensors, which were shown to precisely detect forces at the instrument’s tip in two degrees of freedom perpendicular to the tool axis. This paper presents a new design that employs an additional sensor to capture also the tensile force along the tool axis. The grasping functionality is provided via a compact motorized unit. To compute forces, we investigate two distinct fitting methods: a linear regression and a nonlinear fitting based on second-order Bernstein polynomials. We carry out experiments to test the repeatability of sensor outputs, calibrate the sensor and validate its performance. Results demonstrate sensor wavelength repeatability within 2 pm. Although the linear method provides sufficient accuracy in measuring transverse forces, in the axial direction it produces a root mean square (rms) error over 3 mN even for a confined magnitude and direction of forces. On the other hand, the nonlinear method provides a more consistent and accurate measurement of both the transverse and axial forces for the entire force range (0–25 mN). Validation including random samples shows that our tool with the nonlinear force computation method can predict 3-D forces with an rms error under 0.15 mN in the transverse plane and within 2 mN accuracy in the axial direction. PMID:28736508

Comment on "Scaling regimes and linear/nonlinear responses of last millennium climate to volcanic and solar forcing" by S. Lovejoy and C. Varotsos (2016)

NASA Astrophysics Data System (ADS)

Rypdal, Kristoffer; Rypdal, Martin

2016-07-01

Lovejoy and Varotsos (2016) (L&V) analyse the temperature response to solar, volcanic, and solar plus volcanic forcing in the Zebiak-Cane (ZC) model, and to solar and solar plus volcanic forcing in the Goddard Institute for Space Studies (GISS) E2-R model. By using a simple wavelet filtering technique they conclude that the responses in the ZC model combine subadditively on timescales from 50 to 1000 years. Nonlinear response on shorter timescales is claimed by analysis of intermittencies in the forcing and the temperature signal for both models. The analysis of additivity in the ZC model suffers from a confusing presentation of results based on an invalid approximation, and from ignoring the effect of internal variability. We present tests without this approximation which are not able to detect nonlinearity in the response, even without accounting for internal variability. We also demonstrate that internal variability will appear as subadditivity if it is not accounted for. L&V's analysis of intermittencies is based on a mathematical result stating that the intermittencies of forcing and response are the same if the response is linear. We argue that there are at least three different factors that may invalidate the application of this result for these data. It is valid only for a power-law response function; it assumes power-law scaling of structure functions of forcing as well as temperature signal; and the internal variability, which is strong at least on the short timescales, will exert an influence on temperature intermittence which is independent of the forcing. We demonstrate by a synthetic example that the differences in intermittencies observed by L&V easily can be accounted for by these effects under the assumption of a linear response. Our conclusion is that the analysis performed by L&V does not present valid evidence for a detectable nonlinear response in the global temperature in these climate models.

X-48C Hybrid - Blended Wing Body Demonstrator

NASA Image and Video Library

2013-02-28

NASA X-48C Hybrid Wing Body aircraft flew over one of the runways laid out on Rogers Dry Lake at Edwards Air Force Base, CA, during a test flight from NASA's Dryden Flight Research Center on Feb. 28, 2013.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Benedek, George; Casparay, Alfred H.

In this project, we are developing a new system for measuring forces within and between nanoscale biological molecules based on mesoscopic springs made of cholesterol helical ribbons. These ribbons self-assemble in a wide variety of complex fluids containing sterol, a mixture of surfactants and water [1] and have spring constants in the range from 0.5 to 500 pN/nm [2-4]. By the end of this project, we have demonstrated that the cholesterol helical ribbons can be used for measuring forces between biological objects and for mapping the strain fields in hydrogels.

Aerodynamic Lifting Force.

ERIC Educational Resources Information Center

Weltner, Klaus

1990-01-01

Describes some experiments showing both qualitatively and quantitatively that aerodynamic lift is a reaction force. Demonstrates reaction forces caused by the acceleration of an airstream and the deflection of an airstream. Provides pictures of demonstration apparatus and mathematical expressions. (YP)

[Supercomputer investigation of the protein-ligand system low-energy minima].

PubMed

Oferkin, I V; Sulimov, A V; Katkova, E V; Kutov, D K; Grigoriev, F V; Kondakova, O A; Sulimov, V B

2015-01-01

The accuracy of the protein-ligand binding energy calculations and ligand positioning is strongly influenced by the choice of the docking target function. This work demonstrates the evaluation of the five different target functions used in docking: functions based on MMFF94 force field and functions based on PM7 quantum-chemical method accounting or without accounting the implicit solvent model (PCM, COSMO or SGB). For these purposes the ligand positions corresponding to the minima of the target function and the experimentally known ligand positions in the protein active site (crystal ligand positions) were compared. Each function was examined on the same test-set of 16 protein-ligand complexes. The new parallelized docking program FLM based on Monte Carlo search algorithm was developed to perform the comprehensive low-energy minima search and to calculate the protein-ligand binding energy. This study demonstrates that the docking target function based on the MMFF94 force field can be used to detect the crystal or near crystal positions of the ligand by the finding the low-energy local minima spectrum of the target function. The importance of solvent accounting in the docking process for the accurate ligand positioning is also shown. The accuracy of the ligand positioning as well as the correlation between the calculated and experimentally determined protein-ligand binding energies are improved when the MMFF94 force field is substituted by the new PM7 method with implicit solvent accounting.

Demonstration of an efficient cooling approach for SBIRS-Low

NASA Astrophysics Data System (ADS)

Nieczkoski, S. J.; Myers, E. A.

2002-05-01

The Space Based Infrared System-Low (SBIRS-Low) segment is a near-term Air Force program for developing and deploying a constellation of low-earth orbiting observation satellites with gimbaled optics cooled to cryogenic temperatures. The optical system design and requirements present unique challenges that make conventional cooling approaches both complicated and risky. The Cryocooler Interface System (CIS) provides a remote, efficient, and interference-free means of cooling the SBIRS-Low optics. Technology Applications Inc. (TAI), through a two-phase Small Business Innovative Research (SBIR) program with Air Force Research Laboratory (AFRL), has taken the CIS from initial concept feasibility through the design, build, and test of a prototype system. This paper presents the development and demonstration testing of the prototype CIS. Prototype system testing has demonstrated the high efficiency of this cooling approach, making it an attractive option for SBIRS-Low and other sensitive optical and detector systems that require low-impact cryogenic cooling.

The Optical Bichromatic Force in Molecular Systems

NASA Astrophysics Data System (ADS)

Aldridge, Leland; Galica, Scott; Eyler, E. E.

2015-05-01

The optical bichromatic force has been demonstrated to be useful for slowing atomic beams much more rapidly than radiative forces. Through numerical simulations, we examine several aspects of applying the bichromatic force to molecular beams. One is the unavoidable existence of out-of-system radiative decay, requiring one or more repumping beams. We find that the average deceleration varies strongly with the repumping intensity, but when using optimal parameters, the force approaches the limiting value allowed by population statistics. Another consideration is the effect of fine and hyperfine structure. We examine a simplified multlevel model based on the B <--> X transition in calcium monofluoride. To circumvent optical pumping into coherent dark states, we include two possible schemes: (1) a skewed dc magnetic field, and (2) rapid optical polarization switching. Our results indicate that the bichromatic force remains a viable option for creating large forces in molecular beams, with a reduction in the peak force by approximately an order of magnitude compared to a two-level atom, but little effect on the velocity range over which the force is effective. We also describe our progress towards experimental tests of the bichromatic force on a molecular beam of CaF. Supported by the National Science Foundation.

Phase locking route behind complex periodic windows in a forced oscillator

NASA Astrophysics Data System (ADS)

Jan, Hengtai; Tsai, Kuo-Ting; Kuo, Li-wei

2013-09-01

Chaotic systems have complex reactions against an external driving force; even in cases with low-dimension oscillators, the routes to synchronization are diverse. We proposed a stroboscope-based method for analyzing driven chaotic systems in their phase space. According to two statistic quantities generated from time series, we could realize the system state and the driving behavior simultaneously. We demonstrated our method in a driven bi-stable system, which showed complex period windows under a proper driving force. With increasing periodic driving force, a route from interior periodic oscillation to phase synchronization through the chaos state could be found. Periodic windows could also be identified and the circumstances under which they occurred distinguished. Statistical results were supported by conditional Lyapunov exponent analysis to show the power in analyzing the unknown time series.

Assessing the accuracy of improved force-matched water models derived from Ab initio molecular dynamics simulations.

PubMed

Köster, Andreas; Spura, Thomas; Rutkai, Gábor; Kessler, Jan; Wiebeler, Hendrik; Vrabec, Jadran; Kühne, Thomas D

2016-07-15

The accuracy of water models derived from ab initio molecular dynamics simulations by means on an improved force-matching scheme is assessed for various thermodynamic, transport, and structural properties. It is found that although the resulting force-matched water models are typically less accurate than fully empirical force fields in predicting thermodynamic properties, they are nevertheless much more accurate than generally appreciated in reproducing the structure of liquid water and in fact superseding most of the commonly used empirical water models. This development demonstrates the feasibility to routinely parametrize computationally efficient yet predictive potential energy functions based on accurate ab initio molecular dynamics simulations for a large variety of different systems. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Input Forces Estimation for Nonlinear Systems by Applying a Square-Root Cubature Kalman Filter.

PubMed

Song, Xuegang; Zhang, Yuexin; Liang, Dakai

2017-10-10

This work presents a novel inverse algorithm to estimate time-varying input forces in nonlinear beam systems. With the system parameters determined, the input forces can be estimated in real-time from dynamic responses, which can be used for structural health monitoring. In the process of input forces estimation, the Runge-Kutta fourth-order algorithm was employed to discretize the state equations; a square-root cubature Kalman filter (SRCKF) was employed to suppress white noise; the residual innovation sequences, a priori state estimate, gain matrix, and innovation covariance generated by SRCKF were employed to estimate the magnitude and location of input forces by using a nonlinear estimator. The nonlinear estimator was based on the least squares method. Numerical simulations of a large deflection beam and an experiment of a linear beam constrained by a nonlinear spring were employed. The results demonstrated accuracy of the nonlinear algorithm.

KSC-07pd3078

NASA Image and Video Library

2007-11-02

KENNEDY SPACE CENTER, FLA. -- During a rehearsal, Brig. Gen. Susan J. Helms, Commander of the 45th Space Wing at Patrick Air Force Base, makes a few comments to kick off the World Space Expo Aerial Salute at NASA's Kennedy Space Center. ): Commemorating humanity's first 50 years in space while looking forward to returning people to the moon and exploring beyond, the expo will showcase various panels, presentations and educational programs, as well as the air show. Participating in the air show are the U.S. Air Force Thunderbirds, U.S. Air Force F-22 Raptor, U.S. Navy F-18 Super Hornet, U.S. Air Force F-15 Eagle, the P-51 Mustang Heritage Flight, and the U.S. Air Force 920th Rescue Wing, which was responsible for Mercury and Gemini capsule recovery. The U.S. Army Golden Knights will also demonstrate precision skydiving. Photo credit: NASA/George Shelton

KSC-06pd0446

NASA Image and Video Library

2006-02-15

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers adjust the first half of the fairing around the Space Technology 5 (ST5) spacecraft. The ST5, which contains three microsatellites with miniaturized redundant components and technologies, is mated to its launch vehicle, Orbital Sciences' Pegasus XL. Each of the ST5 microsatellites will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. Launch of ST5 and the Pegasus XL will be from underneath the belly of an L-1011 carrier aircraft on March 14 from Vandenberg Air Force Base.

X-36 Tailless Fighter Agility Research Aircraft in flight

NASA Image and Video Library

1997-10-30

The lack of a vertical tail on the X-36 technology demonstrator is evident as the remotely piloted aircraft flies a low-altitude research flight above Rogers Dry Lake at Edwards Air Force Base in the California desert on October 30, 1997.

NATURAL ATTENUATION OF FUEL HYDROCARBONS AT MULTIPLE AIR FORCE BASE DEMONSTRATION SITES

EPA Science Inventory

ABSTRACT
A major initiative to evaluate monitored natural attenuation (MNA) of ground-water contaminated with fuel hydrocarbons began in June, 1993, and continued through September, 1999. The main emphasis was to evaluate natural degradation mechanisms to reduce dissolved ...

Letting students discover the power, and the limits, of simple models: Coulomb's law

NASA Astrophysics Data System (ADS)

Bohacek, Peter; Vonk, Matthew; Dill, Joseph; Boehm, Emma

2017-09-01

The inverse-square law pops up all over. It's a simplified model of reality that describes light, sound, gravity, and static electricity. But when it's brought up in class, students are often just handed the equations. They rarely have an opportunity to discover Coulomb's law or Newton's law of gravitation for themselves. It's not hard to understand why. A quantitative demonstration of Coulomb's law can be difficult. The forces are smaller than many force sensors can measure and static electricity tends to be finicky. In addition, off-the-shelf units are expensive or difficult to use. As a result, many instructors skip this lab in favor of qualitative demonstrations or simulations. Adolf Cortel sought to remedy this by designing a straightforward experiment for measuring Coulomb's law using charged metalized-glass spheres (Christmas ornaments) and an electronic balance. Building on Cortel's design, we've made a series of video-based experiments that students can use to discover the relationships that underlie electric force.

Localization and force analysis at the single virus particle level using atomic force microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Chih-Hao; Horng, Jim-Tong; Chang, Jeng-Shian

2012-01-06

Highlights: Black-Right-Pointing-Pointer Localization of single virus particle. Black-Right-Pointing-Pointer Force measurements. Black-Right-Pointing-Pointer Force mapping. -- Abstract: Atomic force microscopy (AFM) is a vital instrument in nanobiotechnology. In this study, we developed a method that enables AFM to simultaneously measure specific unbinding force and map the viral glycoprotein at the single virus particle level. The average diameter of virus particles from AFM images and the specificity between the viral surface antigen and antibody probe were integrated to design a three-stage method that sets the measuring area to a single virus particle before obtaining the force measurements, where the influenza virus was usedmore » as the object of measurements. Based on the purposed method and performed analysis, several findings can be derived from the results. The mean unbinding force of a single virus particle can be quantified, and no significant difference exists in this value among virus particles. Furthermore, the repeatability of the proposed method is demonstrated. The force mapping images reveal that the distributions of surface viral antigens recognized by antibody probe were dispersed on the whole surface of individual virus particles under the proposed method and experimental criteria; meanwhile, the binding probabilities are similar among particles. This approach can be easily applied to most AFM systems without specific components or configurations. These results help understand the force-based analysis at the single virus particle level, and therefore, can reinforce the capability of AFM to investigate a specific type of viral surface protein and its distributions.« less

Comparison of crossover and jab step start techniques for base stealing in baseball.

PubMed

Miyanishi, Tomohisa; Endo, So; Nagahara, Ryu

2017-11-01

Base stealing is an important tactic for increasing the chance of scoring in baseball. This study aimed to compare the crossover step (CS) and jab step (JS) starts for base stealing start performance and to clarify the differences between CS and JS starts in terms of three-dimensional lower extremity joint kinetics. Twelve male baseball players performed CS and JS starts, during which their motion and the force they applied to the ground were simultaneously recorded using a motion-capture system and two force platforms. The results showed that the normalised average forward external power, the average forward-backward force exerted by the left leg, and the forward velocities of the whole body centre of gravity generated by both legs and the left leg were significantly higher for the JS start than for the CS start. Moreover, the positive work done by hip extension during the left leg push-off was two-times greater for the JS start than the CS start. In conclusion, this study has demonstrated that the jab step start may be the better technique for a base stealing start and that greater positive work produced by left hip extension is probably responsible for producing its larger forward ground reaction force.

75 FR 53075 - Science and Technology Reinvention Laboratory Personnel Management Demonstration Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-30

... Demonstration Project, Department of the Air Force, Air Force Research Laboratory (AFRL); Notice #0;#0;Federal... Project, Department of the Air Force, Air Force Research Laboratory (AFRL) AGENCY: Office of the Deputy... amendment changed the amount of time required to be assessed under CCS from 180 to 90 calendar days and was...

Whole-Body Human Inverse Dynamics with Distributed Micro-Accelerometers, Gyros and Force Sensing †

PubMed Central

Latella, Claudia; Kuppuswamy, Naveen; Romano, Francesco; Traversaro, Silvio; Nori, Francesco

2016-01-01

Human motion tracking is a powerful tool used in a large range of applications that require human movement analysis. Although it is a well-established technique, its main limitation is the lack of estimation of real-time kinetics information such as forces and torques during the motion capture. In this paper, we present a novel approach for a human soft wearable force tracking for the simultaneous estimation of whole-body forces along with the motion. The early stage of our framework encompasses traditional passive marker based methods, inertial and contact force sensor modalities and harnesses a probabilistic computational technique for estimating dynamic quantities, originally proposed in the domain of humanoid robot control. We present experimental analysis on subjects performing a two degrees-of-freedom bowing task, and we estimate the motion and kinetics quantities. The results demonstrate the validity of the proposed method. We discuss the possible use of this technique in the design of a novel soft wearable force tracking device and its potential applications. PMID:27213394

Improving single molecule force spectroscopy through automated real-time data collection and quantification of experimental conditions

PubMed Central

Scholl, Zackary N.; Marszalek, Piotr E.

2013-01-01

The benefits of single molecule force spectroscopy (SMFS) clearly outweigh the challenges which include small sample sizes, tedious data collection and introduction of human bias during the subjective data selection. These difficulties can be partially eliminated through automation of the experimental data collection process for atomic force microscopy (AFM). Automation can be accomplished using an algorithm that triages usable force-extension recordings quickly with positive and negative selection. We implemented an algorithm based on the windowed fast Fourier transform of force-extension traces that identifies peaks using force-extension regimes to correctly identify usable recordings from proteins composed of repeated domains. This algorithm excels as a real-time diagnostic because it involves <30 ms computational time, has high sensitivity and specificity, and efficiently detects weak unfolding events. We used the statistics provided by the automated procedure to clearly demonstrate the properties of molecular adhesion and how these properties change with differences in the cantilever tip and protein functional groups and protein age. PMID:24001740

B-spline tight frame based force matching method

NASA Astrophysics Data System (ADS)

Yang, Jianbin; Zhu, Guanhua; Tong, Dudu; Lu, Lanyuan; Shen, Zuowei

2018-06-01

In molecular dynamics simulations, compared with popular all-atom force field approaches, coarse-grained (CG) methods are frequently used for the rapid investigations of long time- and length-scale processes in many important biological and soft matter studies. The typical task in coarse-graining is to derive interaction force functions between different CG site types in terms of their distance, bond angle or dihedral angle. In this paper, an ℓ1-regularized least squares model is applied to form the force functions, which makes additional use of the B-spline wavelet frame transform in order to preserve the important features of force functions. The B-spline tight frames system has a simple explicit expression which is useful for representing our force functions. Moreover, the redundancy of the system offers more resilience to the effects of noise and is useful in the case of lossy data. Numerical results for molecular systems involving pairwise non-bonded, three and four-body bonded interactions are obtained to demonstrate the effectiveness of our approach.

KSC-06pd0181

NASA Image and Video Library

2006-01-17

VANDENBERG AIR FORCE BASE, Calif. — At Vandenberg Air Force Base in California, workers are moving the Space Technology 5 (ST5) spacecraft into Orbital Sciences’ Building 1555. There it will be mated with the Pegasus XL launch vehicle. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0445

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers clean and prepare the fairing to be installed around the Space Technology 5 (ST5) spacecraft. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0438

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers check the Orbital Sciences' Pegasus XL launch vehicle before encapsulation of the Space Technology 5 (ST5) spacecraft. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0186

NASA Image and Video Library

2006-01-18

VANDENBERG AIR FORCE BASE, Calif. — Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, the wrapped Space Technology 5 (ST5) spacecraft is revealed after removal of the shipping container. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0437

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. -Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, a worker checks connections on the Space Technology 5 (ST5) spacecraft before encapsulation with the fairing. The ST5, mated to Orbital Sciences' Pegasus XL launch vehicle, contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0172

NASA Image and Video Library

2006-01-13

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, the three micro-satellites comprising the Space Technology 5 spacecraft are mated and ready for weighing. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0434

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, this closeup shows the Space Technology 5 (ST5) spacecraft's microsatellites mounted on the payload structure. The spacecraft will be enclosed for launch. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0169

NASA Image and Video Library

2006-01-13

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, technicians complete mating of the three micro-satellites on the payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0180

NASA Image and Video Library

2006-01-17

VANDENBERG AIR FORCE BASE, Calif. — At Vandenberg Air Force Base in California, workers are moving the Space Technology 5 (ST5) spacecraft out of the truck into Orbital Sciences’ Building 1555. There it will be mated with the Pegasus XL launch vehicle. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0179

NASA Image and Video Library

2006-01-17

VANDENBERG AIR FORCE BASE, Calif. — At Vandenberg Air Force Base in California, workers are moving the Space Technology 5 (ST5) spacecraft out of the Orbital Sciences Building 836 onto a truck for transfer to Building 1555. There it will be mated with the Pegasus XL launch vehicle. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0177

NASA Image and Video Library

2006-01-16

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, a scale attached to a crane is ready to lift the payload support structure with the three micro-satellites comprising the Space Technology 5 (ST5) spacecraft. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0161

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers move lift one of three micro-satellites to prepare it for mating to the payload support structure. The three satellites that make up the Space Technology 5 spacecraft, called ST5, will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0173

NASA Image and Video Library

2006-01-16

VANDENBERG AIR FORCE BASE, Calif. — In In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, the payload support structure with the three micro-satellites comprising the Space Technology 5 (ST5) spacecraft has been raised to vertical to be weighed. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0442

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers prepare the fairing to be installed around the Space Technology 5 (ST5) spacecraft. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0170

NASA Image and Video Library

2006-01-13

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, technicians complete mating of the three micro-satellites on the payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0175

NASA Image and Video Library

2006-01-16

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers prepare the scale that will be used to weigh the three micro-satellites comprising the Space Technology 5 (ST5) spacecraft. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0162

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers guide one of three micro-satellites onto a payload support structure. The three satellites that make up the Space Technology 5 spacecraft, called ST5, will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0182

NASA Image and Video Library

2006-01-17

VANDENBERG AIR FORCE BASE, Calif. — At Vandenberg Air Force Base in California, workers are moving the Space Technology 5 (ST5) spacecraft into Orbital Sciences’ Building 1555. There it will be mated with the Pegasus XL launch vehicle. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0167

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers are mating a third satellite onto the payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0185

NASA Image and Video Library

2006-01-18

VANDENBERG AIR FORCE BASE, Calif. — Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, the wrapped Space Technology 5 (ST5) spacecraft is revealed after removal of the shipping container. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0176

NASA Image and Video Library

2006-01-16

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, a scale is attached to a crane that lifts the payload support structure with the three micro-satellites comprising the Space Technology 5 (ST5) spacecraft. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0168

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, three micro-satellites are mounted on the payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0163

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers secure one of three micro-satellites onto a payload support structure. The three satellites that make up the Space Technology 5 spacecraft, called ST5, will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0183

NASA Image and Video Library

2006-01-18

VANDENBERG AIR FORCE BASE, Calif. — Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers attach the wires to lift the shipping container surrounding the Space Technology 5 (ST5) spacecraft. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0441

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers clean and prepare the fairing to be installed around the Space Technology 5 (ST5) spacecraft. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0174

NASA Image and Video Library

2006-01-16

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers prepare the scale that will be used to weigh the three micro-satellites comprising the Space Technology 5 (ST5) spacecraft. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0439

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, one half of the fairing is being installed around the Space Technology 5 (ST5) spacecraft. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0171

NASA Image and Video Library

2006-01-13

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, technicians complete mating of the three micro-satellites on the payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0431

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, the Space Technology 5 (ST5) spacecraft waits for encapsulation after mating with the Orbital Sciences' Pegasus XL launch vehicle. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0184

NASA Image and Video Library

2006-01-18

VANDENBERG AIR FORCE BASE, Calif. — Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers assure the shipping container surrounding the Space Technology 5 (ST5) spacecraft is lifted safely. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0440

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers adjust the first half of the fairing being installed around the Space Technology 5 (ST5) spacecraft. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0178

NASA Image and Video Library

2006-01-16

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers keep close watch as the payload support structure with the three micro-satellites comprising the Space Technology 5 (ST5) spacecraft is lifted and weighed. ST5 will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

Design Optimisation of a Magnetic Field Based Soft Tactile Sensor

PubMed Central

Raske, Nicholas; Kow, Junwai; Alazmani, Ali; Ghajari, Mazdak; Culmer, Peter; Hewson, Robert

2017-01-01

This paper investigates the design optimisation of a magnetic field based soft tactile sensor, comprised of a magnet and Hall effect module separated by an elastomer. The aim was to minimise sensitivity of the output force with respect to the input magnetic field; this was achieved by varying the geometry and material properties. Finite element simulations determined the magnetic field and structural behaviour under load. Genetic programming produced phenomenological expressions describing these responses. Optimisation studies constrained by a measurable force and stable loading conditions were conducted; these produced Pareto sets of designs from which the optimal sensor characteristics were selected. The optimisation demonstrated a compromise between sensitivity and the measurable force, a fabricated version of the optimised sensor validated the improvements made using this methodology. The approach presented can be applied in general for optimising soft tactile sensor designs over a range of applications and sensing modes. PMID:29099787

Experimental Tracking of Limit-Point Bifurcations and Backbone Curves Using Control-Based Continuation

NASA Astrophysics Data System (ADS)

Renson, Ludovic; Barton, David A. W.; Neild, Simon A.

Control-based continuation (CBC) is a means of applying numerical continuation directly to a physical experiment for bifurcation analysis without the use of a mathematical model. CBC enables the detection and tracking of bifurcations directly, without the need for a post-processing stage as is often the case for more traditional experimental approaches. In this paper, we use CBC to directly locate limit-point bifurcations of a periodically forced oscillator and track them as forcing parameters are varied. Backbone curves, which capture the overall frequency-amplitude dependence of the system’s forced response, are also traced out directly. The proposed method is demonstrated on a single-degree-of-freedom mechanical system with a nonlinear stiffness characteristic. Results are presented for two configurations of the nonlinearity — one where it exhibits a hardening stiffness characteristic and one where it exhibits softening-hardening.

Bubble-based acoustic radiation force using chirp insonation to reduce standing wave effects.

PubMed

Erpelding, Todd N; Hollman, Kyle W; O'Donnell, Matthew

2007-02-01

Bubble-based acoustic radiation force can measure local viscoelastic properties of tissue. High intensity acoustic waves applied to laser-generated bubbles induce displacements inversely proportional to local Young's modulus. In certain instances, long pulse durations are desirable but are susceptible to standing wave artifacts, which corrupt displacement measurements. Chirp pulse acoustic radiation force was investigated as a method to reduce standing wave artifacts. Chirp pulses with linear frequency sweep magnitudes of 100, 200 and 300 kHz centered around 1.5 MHz were applied to glass beads within gelatin phantoms and laser-generated bubbles within porcine lenses. The ultrasound transducer was translated axially to vary standing wave conditions, while comparing displacements using chirp pulses and 1.5 MHz tone burst pulses of the same duration and peak rarefactional pressure. Results demonstrated significant reduction in standing wave effects using chirp pulses, with displacement proportional to acoustic intensity and bubble size.

Bubble-Based Acoustic Radiation Force Using Chirp Insonation to Reduce Standing Wave Effects

PubMed Central

Erpelding, Todd N.; Hollman, Kyle W.; O’Donnell, Matthew

2007-01-01

Bubble-based acoustic radiation force can measure local viscoelastic properties of tissue. High intensity acoustic waves applied to laser-generated bubbles induce displacements inversely proportional to local Young’s modulus. In certain instances, long pulse durations are desirable but are susceptible to standing wave artifacts, which corrupt displacement measurements. Chirp pulse acoustic radiation force was investigated as a method to reduce standing wave artifacts. Chirp pulses with linear frequency sweep magnitudes of 100, 200, and 300 kHz centered around 1.5 MHz were applied to glass beads within gelatin phantoms and laser-generated bubbles within porcine lenses. The ultrasound transducer was translated axially to vary standing wave conditions, while comparing displacements using chirp pulses and 1.5 MHz tone burst pulses of the same duration and peak rarefactional pressure. Results demonstrated significant reduction in standing wave effects using chirp pulses, with displacement proportional to acoustic intensity and bubble size. PMID:17306697

Phase measurement by using a forced delay-line oscillator and its application for an acoustic fiber sensor.

PubMed

Fleyer, Michael; Horowitz, Moshe

2018-04-02

We demonstrate, theoretically and experimentally, a new method to measure small changes in the cavity length of oscillators. The method is based on the high sensitivity of the phase of forced delay-line oscillators to changes in their cavity length. The oscillator phase is directly detected by mixing the oscillator output with the injected signal. We describe a comprehensive theoretical model for studying the signal and the noise at the output of a general forced delay-line oscillator with an instantaneous gain saturation and an amplitude-to-phase conversion. The results indicate that the magnitude and the bandwidth of the oscillator response to a small perturbation can be controlled by adjusting the injection ratio and the injected frequency. For signals with a frequency that is smaller than the device bandwidth, the oscillator noise is dominated by the noise of the injected signal. This noise is highly suppressed by mixing the oscillator output with the injected signal. Hence, the device sensitivity at frequencies below its bandwidth is limited only by the internal noise that is added in a single roundtrip in the oscillator cavity. We demonstrate the use of a forced oscillator as an acoustic fiber sensor in an optoelectronic oscillator. A good agreement is obtained between theory and experiments. The magnitude of the output signal can be controlled by adjusting the injection ratio while the noise power at low frequencies is not enhanced as in sensors that are based on a free-running oscillator.

Harnessing the polariton drag effect to design an electrically controlled optical switch.

PubMed

Berman, Oleg L; Kezerashvili, Roman Ya; Kolmakov, German V

2014-10-28

We propose a design of a Y-shaped electrically controlled optical switch based on the studies of propagation of an exciton-polariton condensate in a patterned optical microcavity with an embedded quantum well. The polaritons are driven by a time-independent force due to the microcavity wedge shape and by a time-dependent drag force owing to the interaction of excitons in a quantum well and the electric current running in a neighboring quantum well. It is demonstrated that by applying the drag force one can direct more than 90% of the polariton flow toward the desired branch of the switch with no hysteresis. By considering the transient dynamics of the polariton condensate, we estimate the response speed of the switch as 9.1 GHz. We also propose a design of the polariton switch in a flat microcavity based on the geometrically identical Y-shaped quantum wells where the polariton flow is only induced by the drag force. The latter setup enables one to design a multiway switch that can act as an electrically controlled optical transistor with on and off functions. Finally, we performed the simulations for a microcavity with an embedded gapped graphene layer and demonstrated that in this case the response speed of the switch can be increased up to 14 GHz for the same switch size. The simulations also show that the energy gap in the quasiparticle spectrum in graphene can be utilized as an additional parameter that controls the propagation of the signals in the switch.

Logistics Force Planner Assistant (Log Planner)

DTIC Science & Technology

1989-09-01

elements. The system is implemented on a MS-DOS based microcomputer, using the "Knowledge Pro’ software tool., 20 DISTRIBUTION/AVAILABILITY OF... service support structure. 3. A microcomputer-based knowledge system was developed and successfully demonstrated. Four modules of information are...combat service support (CSS) units planning process to Army Staff logistics planners. Personnel newly assigned to logistics planning need an

The Role of Binding Site on the Mechanical Unfolding Mechanism of Ubiquitin

NASA Astrophysics Data System (ADS)

Cao, Penghui; Yoon, Gwonchan; Tao, Weiwei; Eom, Kilho; Park, Harold S.

2015-03-01

We apply novel atomistic simulations based on potential energy surface exploration to investigate the constant force-induced unfolding of ubiquitin. At the experimentally-studied force clamping level of 100 pN, we find a new unfolding mechanism starting with the detachment between β5 and β3 involving the binding site of ubiquitin, the Ile44 residue. This new unfolding pathway leads to the discovery of new intermediate configurations, which correspond to the end-to-end extensions previously seen experimentally. More importantly, it demonstrates the novel finding that the binding site of ubiquitin can be responsible not only for its biological functions, but also its unfolding dynamics. We also report in contrast to previous single molecule constant force experiments that when the clamping force becomes smaller than about 300 pN, the number of intermediate configurations increases dramatically, where almost all unfolding events at 100 pN involve an intermediate configuration. By directly calculating the life times of the intermediate configurations from the height of the barriers that were crossed on the potential energy surface, we demonstrate that these intermediate states were likely not observed experimentally due to their lifetimes typically being about two orders of magnitude smaller than the experimental temporal resolution.

Objective Assessment of Joint Stiffness: A Clinically Oriented Hardware and Software Device with an Application to the Shoulder Joint.

PubMed

McQuade, Kevin; Price, Robert; Liu, Nelson; Ciol, Marcia A

2012-08-30

Examination of articular joints is largely based on subjective assessment of the "end-feel" of the joint in response to manually applied forces at different joint orientations. This technical report aims to describe the development of an objective method to examine joints in general, with specific application to the shoulder, and suitable for clinical use. We adapted existing hardware and developed laptop-based software to objectively record the force/displacement behavior of the glenohumeral joint during three common manual joint examination tests with the arm in six positions. An electromagnetic tracking system recorded three-dimensional positions of sensors attached to a clinician examiner and a patient. A hand-held force transducer recorded manually applied translational forces. The force and joint displacement were time-synchronized and the joint stiffness was calculated as a quantitative representation of the joint "end-feel." A methodology and specific system checks were developed to enhance clinical testing reproducibility and precision. The device and testing protocol were tested on 31 subjects (15 with healthy shoulders, and 16 with a variety of shoulder impairments). Results describe the stiffness responses, and demonstrate the feasibility of using the device and methods in clinical settings.

ForceGen 3D structure and conformer generation: from small lead-like molecules to macrocyclic drugs

NASA Astrophysics Data System (ADS)

Cleves, Ann E.; Jain, Ajay N.

2017-05-01

We introduce the ForceGen method for 3D structure generation and conformer elaboration of drug-like small molecules. ForceGen is novel, avoiding use of distance geometry, molecular templates, or simulation-oriented stochastic sampling. The method is primarily driven by the molecular force field, implemented using an extension of MMFF94s and a partial charge estimator based on electronegativity-equalization. The force field is coupled to algorithms for direct sampling of realistic physical movements made by small molecules. Results are presented on a standard benchmark from the Cambridge Crystallographic Database of 480 drug-like small molecules, including full structure generation from SMILES strings. Reproduction of protein-bound crystallographic ligand poses is demonstrated on four carefully curated data sets: the ConfGen Set (667 ligands), the PINC cross-docking benchmark (1062 ligands), a large set of macrocyclic ligands (182 total with typical ring sizes of 12-23 atoms), and a commonly used benchmark for evaluating macrocycle conformer generation (30 ligands total). Results compare favorably to alternative methods, and performance on macrocyclic compounds approaches that observed on non-macrocycles while yielding a roughly 100-fold speed improvement over alternative MD-based methods with comparable performance.

The fast multipole method and point dipole moment polarizable force fields.

PubMed

Coles, Jonathan P; Masella, Michel

2015-01-14

We present an implementation of the fast multipole method for computing Coulombic electrostatic and polarization forces from polarizable force-fields based on induced point dipole moments. We demonstrate the expected O(N) scaling of that approach by performing single energy point calculations on hexamer protein subunits of the mature HIV-1 capsid. We also show the long time energy conservation in molecular dynamics at the nanosecond scale by performing simulations of a protein complex embedded in a coarse-grained solvent using a standard integrator and a multiple time step integrator. Our tests show the applicability of fast multipole method combined with state-of-the-art chemical models in molecular dynamical systems.

ED07-0139-30

NASA Image and Video Library

2007-06-23

NASA's Ikhana unmanned science demonstration aircraft prepares for landing as it arrives at Edwards Air Force Base, Calif. NASA took possession of the new aircraft in November, 2006, and it arrived at its new home at NASA's Dryden Flight Reseach Center at Edwards AFB, on June 23, 2007.

ED07-0139-19

NASA Image and Video Library

2007-06-23

NASA's Ikhana unmanned science demonstration aircraft in flight during the ferry flight to its new home at the Dryden Flight Research Center. NASA took possession of the new aircraft in November, 2006, and it arrived at the NASA center at Edwards Air Force Base, Calif., on June 23, 2007.

FIELD DEMONSTRATION STUDIES OF SURFACTANT-ENHANCED SOLUBILIZATION AND MOBILIZATION AT HILL AIR FORCE BASE, UTAH

EPA Science Inventory

Surfactant-enhanced subsurface remediation can dramatically improve contaminant removal rates compared to the traditional pump-and-treat technology. Surfactants can be used to significantly enhance the solubilization of non-aqueous phase liquids (NAPL) constituents, or they can b...

Field-scale demonstration of induced biogeochemical reductive dechlorination at Dover Air Force Base, Dover, Delaware

NASA Astrophysics Data System (ADS)

Kennedy, Lonnie G.; Everett, Jess W.; Becvar, Erica; DeFeo, Donald

2006-11-01

Biogeochemical reductive dechlorination (BiRD) is a new remediation approach for chlorinated aliphatic hydrocarbons (CAHs). The approach stimulates common sulfate-reducing soil bacteria, facilitating the geochemical conversion of native iron minerals into iron sulfides. Iron sulfides have the ability to chemically reduce many common CAH compounds including PCE, TCE, DCE, similar to zero valent iron (Fe 0). Results of a field test at Dover Air Force Base, Dover, Delaware, are given in this paper. BiRD was stimulated by direct injection of Epson salt (MgSO 4·7H 2O) and sodium (L) lactate (NaC 3H 5O 3) in five injection wells. Sediment was sampled before and 8 months after injection. Significant iron sulfide minerals developed in the sandy aquifer matrix. From ground water analyses, treatment began a few weeks after injection with up to 95% reduction in PCE, TCE, and cDCE in less than 1 year. More complete CAH treatment is likely at a larger scale than this demonstration.

One-Channel Surface Electromyography Decomposition for Muscle Force Estimation.

PubMed

Sun, Wentao; Zhu, Jinying; Jiang, Yinlai; Yokoi, Hiroshi; Huang, Qiang

2018-01-01

Estimating muscle force by surface electromyography (sEMG) is a non-invasive and flexible way to diagnose biomechanical diseases and control assistive devices such as prosthetic hands. To estimate muscle force using sEMG, a supervised method is commonly adopted. This requires simultaneous recording of sEMG signals and muscle force measured by additional devices to tune the variables involved. However, recording the muscle force of the lost limb of an amputee is challenging, and the supervised method has limitations in this regard. Although the unsupervised method does not require muscle force recording, it suffers from low accuracy due to a lack of reference data. To achieve accurate and easy estimation of muscle force by the unsupervised method, we propose a decomposition of one-channel sEMG signals into constituent motor unit action potentials (MUAPs) in two steps: (1) learning an orthogonal basis of sEMG signals through reconstruction independent component analysis; (2) extracting spike-like MUAPs from the basis vectors. Nine healthy subjects were recruited to evaluate the accuracy of the proposed approach in estimating muscle force of the biceps brachii. The results demonstrated that the proposed approach based on decomposed MUAPs explains more than 80% of the muscle force variability recorded at an arbitrary force level, while the conventional amplitude-based approach explains only 62.3% of this variability. With the proposed approach, we were also able to achieve grip force control of a prosthetic hand, which is one of the most important clinical applications of the unsupervised method. Experiments on two trans-radial amputees indicated that the proposed approach improves the performance of the prosthetic hand in grasping everyday objects.

Exploring the impact of visual and movement based priming on a motor intervention in the acute phase post-stroke in persons with severe hemiparesis of the upper extremity.

PubMed

Patel, Jigna; Qiu, Qinyin; Yarossi, Mathew; Merians, Alma; Massood, Supriya; Tunik, Eugene; Adamovich, Sergei; Fluet, Gerard

2017-07-01

Explore the potential benefits of using priming methods prior to an active hand task in the acute phase post-stroke in persons with severe upper extremity hemiparesis. Five individuals were trained using priming techniques including virtual reality (VR) based visual mirror feedback and contralaterally controlled passive movement strategies prior to training with an active pinch force modulation task. Clinical, kinetic, and neurophysiological measurements were taken pre and post the training period. Clinical measures were taken at six months post training. The two priming simulations and active training were well tolerated early after stroke. Priming effects were suggested by increased maximal pinch force immediately after visual and movement based priming. Despite having no clinically observable movement distally, the subjects were able to volitionally coordinate isometric force and muscle activity (EMG) in a pinch tracing task. The Root Mean Square Error (RMSE) of force during the pinch trace task gradually decreased over the training period suggesting learning may have occurred. Changes in motor cortical neurophysiology were seen in the unaffected hemisphere using Transcranial Magnetic Stimulation (TMS) mapping. Significant improvements in motor recovery as measured by the Action Research Arm Test (ARAT) and the Upper Extremity Fugl Meyer Assessment (UEFMA) were demonstrated at six months post training by three of the five subjects. This study suggests that an early hand-based intervention using visual and movement based priming activities and a scaled motor task allows participation by persons without the motor control required for traditionally presented rehabilitation and testing. Implications for Rehabilitation Rehabilitation of individuals with severely paretic upper extremities after stroke is challenging due to limited movement capacity and few options for therapeutic training. Long-term functional recovery of the arm after stroke depends on early return of active hand control, establishing a need for acute training methods focused distally. This study demonstrates the feasibility of an early hand-based intervention using virtual reality based priming and scaled motor activities which can allow for participation by persons without the motor control required for traditionally presented rehabilitation and testing.

Force-momentum-based self-guided Langevin dynamics: A rapid sampling method that approaches the canonical ensemble

NASA Astrophysics Data System (ADS)

Wu, Xiongwu; Brooks, Bernard R.

2011-11-01

The self-guided Langevin dynamics (SGLD) is a method to accelerate conformational searching. This method is unique in the way that it selectively enhances and suppresses molecular motions based on their frequency to accelerate conformational searching without modifying energy surfaces or raising temperatures. It has been applied to studies of many long time scale events, such as protein folding. Recent progress in the understanding of the conformational distribution in SGLD simulations makes SGLD also an accurate method for quantitative studies. The SGLD partition function provides a way to convert the SGLD conformational distribution to the canonical ensemble distribution and to calculate ensemble average properties through reweighting. Based on the SGLD partition function, this work presents a force-momentum-based self-guided Langevin dynamics (SGLDfp) simulation method to directly sample the canonical ensemble. This method includes interaction forces in its guiding force to compensate the perturbation caused by the momentum-based guiding force so that it can approximately sample the canonical ensemble. Using several example systems, we demonstrate that SGLDfp simulations can approximately maintain the canonical ensemble distribution and significantly accelerate conformational searching. With optimal parameters, SGLDfp and SGLD simulations can cross energy barriers of more than 15 kT and 20 kT, respectively, at similar rates for LD simulations to cross energy barriers of 10 kT. The SGLDfp method is size extensive and works well for large systems. For studies where preserving accessible conformational space is critical, such as free energy calculations and protein folding studies, SGLDfp is an efficient approach to search and sample the conformational space.

Vertical electrostatic force in MEMS cantilever IR sensor

NASA Astrophysics Data System (ADS)

Rezadad, Imen; Boroumand Azad, Javaneh; Smith, Evan M.; Alhasan, Ammar; Peale, Robert E.

2014-06-01

A MEMS cantilever IR detector that repetitively lifts from the surface under the influence of a saw-tooth electrostatic force, where the contact duty cycle is a measure of the absorbed IR radiation, is analyzed. The design is comprised of three parallel conducting plates. Fixed buried and surface plates are held at opposite potential. A moveable cantilever is biased the same as the surface plate. Calculations based on energy methods with position-dependent capacity and electrostatic induction coefficients demonstrate the upward sign of the force on the cantilever and determine the force magnitude. 2D finite element method calculations of the local fields confirm the sign of the force and determine its distribution across the cantilever. The upward force is maximized when the surface plate is slightly larger than the other two. The electrostatic repulsion is compared with Casimir sticking force to determine the maximum useful contact area. MEMS devices were fabricated and the vertical displacement of the cantilever was observed in a number of experiments. The approach may be applied also to MEMS actuators and micromirrors.

Non-linear scale interactions in a forced turbulent boundary layer

NASA Astrophysics Data System (ADS)

Duvvuri, Subrahmanyam; McKeon, Beverley

2015-11-01

A strong phase-organizing influence exerted by a single synthetic large-scale spatio-temporal mode on directly-coupled (through triadic interactions) small scales in a turbulent boundary layer forced by a spatially-impulsive dynamic wall-roughness patch was previously demonstrated by the authors (J. Fluid Mech. 2015, vol. 767, R4). The experimental set-up was later enhanced to allow for simultaneous forcing of multiple scales in the flow. Results and analysis are presented from a new set of novel experiments where two distinct large scales are forced in the flow by a dynamic wall-roughness patch. The internal non-linear forcing of two other scales with triadic consistency to the artificially forced large scales, corresponding to sum and difference in wavenumbers, is dominated by the latter. This allows for a forcing-response (input-output) type analysis of the two triadic scales, and naturally lends itself to a resolvent operator based model (e.g. McKeon & Sharma, J. Fluid Mech. 2010, vol. 658, pp. 336-382) of the governing Navier-Stokes equations. The support of AFOSR (grant #FA 9550-12-1-0469, program manager D. Smith) is gratefully acknowledged.

6Li in a three-body model with realistic Forces: Separable versus nonseparable approach

NASA Astrophysics Data System (ADS)

Hlophe, L.; Lei, Jin; Elster, Ch.; Nogga, A.; Nunes, F. M.

2017-12-01

Background: Deuteron induced reactions are widely used to probe nuclear structure and astrophysical information. Those (d ,p ) reactions may be viewed as three-body reactions and described with Faddeev techniques. Purpose: Faddeev equations in momentum space have a long tradition of utilizing separable interactions in order to arrive at sets of coupled integral equations in one variable. However, it needs to be demonstrated that their solution based on separable interactions agrees exactly with solutions based on nonseparable forces. Methods: Momentum space Faddeev equations are solved with nonseparable and separable forces as coupled integral equations. Results: The ground state of 6Li is calculated via momentum space Faddeev equations using the CD-Bonn neutron-proton force and a Woods-Saxon type neutron(proton)-4He force. For the latter the Pauli-forbidden S -wave bound state is projected out. This result is compared to a calculation in which the interactions in the two-body subsystems are represented by separable interactions derived in the Ernst-Shakin-Thaler (EST) framework. Conclusions: We find that calculations based on the separable representation of the interactions and the original interactions give results that agree to four significant figures for the binding energy, provided that energy and momentum support points of the EST expansion are chosen independently. The momentum distributions computed in both approaches also fully agree with each other.

A droplet-based passive force sensor for remote tactile sensing applications

NASA Astrophysics Data System (ADS)

Nie, Baoqing; Yao, Ting; Zhang, Yiqiu; Liu, Jian; Chen, Xinjian

2018-01-01

A droplet-based flexible wireless force sensor has been developed for remote tactile-sensing applications. By integration of a droplet-based capacitive sensing unit and two circular planar coils, this inductor-capacitor (LC) passive sensor offers a platform for the mechanical force detection in a wireless transmitting mode. Under external loads, the membrane surface of the sensor deforms the underlying elastic droplet uniformly, introducing a capacitance response in tens of picofarads. The LC circuit transduces the applied force into corresponding variations of its resonance frequency, which is detected by an external electromagnetic coupling coil. Specifically, the liquid droplet features a mechanosensitive plasticity, which results in an increased device sensitivity as high as 2.72 MHz N-1. The high dielectric property of the droplet endows our sensor with high tolerance for noise and large capacitance values (20-40 pF), the highest value in the literature for the LC passive devices in comparable dimensions. It achieves excellent reproducibility under periodical loads ranging from 0 to 1.56 N and temperature fluctuations ranging from 10 °C to 55 °C. As an interesting conceptual demonstration, the flexible device has been configured into a fingertip-amounted setting in a highly compact package (of 11 mm × 11 mm × 0.25 mm) for remote contact force sensing in the table tennis game.

Pneumatic artificial muscle actuators for compliant robotic manipulators

NASA Astrophysics Data System (ADS)

Robinson, Ryan Michael

Robotic systems are increasingly being utilized in applications that require interaction with humans. In order to enable safe physical human-robot interaction, light weight and compliant manipulation are desirable. These requirements are problematic for many conventional actuation systems, which are often heavy, and typically use high stiffness to achieve high performance, leading to large impact forces upon collision. However, pneumatic artificial muscles (PAMs) are actuators that can satisfy these safety requirements while offering power-to-weight ratios comparable to those of conventional actuators. PAMs are extremely lightweight actuators that produce force in response to pressurization. These muscles demonstrate natural compliance, but have a nonlinear force-contraction profile that complicates modeling and control. This body of research presents solutions to the challenges associated with the implementation of PAMs as actuators in robotic manipulators, particularly with regard to modeling, design, and control. An existing PAM force balance model was modified to incorporate elliptic end geometry and a hyper-elastic constitutive relationship, dramatically improving predictions of PAM behavior at high contraction. Utilizing this improved model, two proof-of-concept PAM-driven manipulators were designed and constructed; design features included parallel placement of actuators and a tendon-link joint design. Genetic algorithm search heuristics were employed to determine an optimal joint geometry; allowing a manipulator to achieve a desired torque profile while minimizing the required PAM pressure. Performance of the manipulators was evaluated in both simulation and experiment employing various linear and nonlinear control strategies. These included output feedback techniques, such as proportional-integral-derivative (PID) and fuzzy logic, a model-based control for computed torque, and more advanced controllers, such as sliding mode, adaptive sliding mode, and adaptive neural network control. Results demonstrated the benefits of an accurate model in model-based control, and the advantages of adaptive neural network control when a model is unavailable or variations in payload are expected. Lastly, a variable recruitment strategy was applied to a group of parallel muscles actuating a common joint. Increased manipulator efficiency was observed when fewer PAMs were activated, justifying the use of variable recruitment strategies. Overall, this research demonstrates the benefits of pneumatic artificial muscles as actuators in robotics applications. It demonstrates that PAM-based manipulators can be well-modeled and can achieve high tracking accuracy over a wide range of payloads and inputs while maintaining natural compliance.

Optical levitation particle delivery system for a dual beam fiber optic trap.

PubMed

Gauthier, R C; Frangioudakis, A

2000-01-01

We combine a radiation-pressure-based levitation system with a dual fiber, laser trapping system to demonstrate the potential of delivering single particles into the fiber trap. The forces versus position and the trajectory of the particle subjected to the laser beams are examined with an enhanced ray optics model. A sequence of video images taken from the experimental apparatus demonstrates the principle of particle delivery, trapping, and further manipulation.

Use of patient specific 3D printed (3DP) neurovascular phantoms for mechanical assessment of devices used in image guided minimally invasive procedures

NASA Astrophysics Data System (ADS)

Tabaczynski, Janelle R.; Stoll, Thomas; Shepard, Lauren; Siddiqui, Mohamed I. G.; Karkhanis, Nitant V.; Sommer, Kelsey; Siddiqui, Adnan H.; Ionita, Ciprian N.

2018-03-01

Patient-specific 3D printed phantoms (3DP) can reproduce accurate patient geometry and provide precise tools for Endovascular Image Guided Interventions (EIGI) simulations. We propose to build and test 3DP phantoms which mimic the arterial wall elasticity and surface properties and demonstrate their utility in comprehensive EIGI simulations. 3DP idealized and patient specific vascular phantoms were manufactured using Stratasys Objet 500 Connex 3. The idealized phantoms were created using a sine wave shape, patient specific phantoms were based on CT- angiography volumes. The phantoms were coated with a hydrophilic material to mimic vascular surface properties. We tested various endovascular procedures using an Interventional Device Testing Equipment (IDTE) 2000 and measured push/pull force used to actuate endovascular devices during EIGIs. The force needed to advance devices in neurovascular phantoms varied based on tortuosity, material and coating, ranging from -3 to 21 grams-force. Hydrophilic coating reduced maximum force from 21 to 4.8 grams-force in the same model. IDTE 2000 results of neurovascular models were compared to hand manipulation of guidewire access using a six-axis force sensor with forces ranging from -50 to 440 grams. The clot retriever tested in carotid models experienced most friction around tortuous bends ranging from -65 to -90 grams-force, with increasing rigidity of materials creating increased friction. Sine wave model forces varied from -2 to 105 grams. 3DP allows manufacturing of vascular phantoms with precise mechanical and surface properties which can be used for EIGI simulations for imaging protocol optimization and device behavior assessment.

Toward Improved Force-Field Accuracy through Sensitivity Analysis of Host-Guest Binding Thermodynamics

PubMed Central

Yin, Jian; Fenley, Andrew T.; Henriksen, Niel M.; Gilson, Michael K.

2015-01-01

Improving the capability of atomistic computer models to predict the thermodynamics of noncovalent binding is critical for successful structure-based drug design, and the accuracy of such calculations remains limited by non-optimal force field parameters. Ideally, one would incorporate protein-ligand affinity data into force field parametrization, but this would be inefficient and costly. We now demonstrate that sensitivity analysis can be used to efficiently tune Lennard-Jones parameters of aqueous host-guest systems for increasingly accurate calculations of binding enthalpy. These results highlight the promise of a comprehensive use of calorimetric host-guest binding data, along with existing validation data sets, to improve force field parameters for the simulation of noncovalent binding, with the ultimate goal of making protein-ligand modeling more accurate and hence speeding drug discovery. PMID:26181208

Small strain multiphase-field model accounting for configurational forces and mechanical jump conditions

NASA Astrophysics Data System (ADS)

Schneider, Daniel; Schoof, Ephraim; Tschukin, Oleg; Reiter, Andreas; Herrmann, Christoph; Schwab, Felix; Selzer, Michael; Nestler, Britta

2018-03-01

Computational models based on the phase-field method have become an essential tool in material science and physics in order to investigate materials with complex microstructures. The models typically operate on a mesoscopic length scale resolving structural changes of the material and provide valuable information about the evolution of microstructures and mechanical property relations. For many interesting and important phenomena, such as martensitic phase transformation, mechanical driving forces play an important role in the evolution of microstructures. In order to investigate such physical processes, an accurate calculation of the stresses and the strain energy in the transition region is indispensable. We recall a multiphase-field elasticity model based on the force balance and the Hadamard jump condition at the interface. We show the quantitative characteristics of the model by comparing the stresses, strains and configurational forces with theoretical predictions in two-phase cases and with results from sharp interface calculations in a multiphase case. As an application, we choose the martensitic phase transformation process in multigrain systems and demonstrate the influence of the local homogenization scheme within the transition regions on the resulting microstructures.

Compact variable-temperature scanning force microscope.

PubMed

Chuang, Tien-Ming; de Lozanne, Alex

2007-05-01

A compact design for a cryogenic variable-temperature scanning force microscope using a fiber-optic interferometer to measure cantilever deflection is presented. The tip-sample coarse approach and the lateral tip positioning are performed by piezoelectric positioners in situ. The microscope has been operated at temperatures between 6 and 300 K. It is designed to fit into an 8 T superconducting magnet with the field applied in the out-of-plane direction. The results of scanning in various modes are demonstrated, showing contrast based on magnetic field gradients or surface potentials.

Chemico-therapeutic approach to prevention of dental caries. [using stannous fluoride gel

NASA Technical Reports Server (NTRS)

Shannon, I. L.

1975-01-01

The program of chemical preventive dentistry is based primarily upon the development of a procedure for stabilizing stannous fluoride in solution by forcing it into glycerin. New topical fluoride treatment concentrates, fluoride containing gels and prophylaxis pastes, as well as a completely stable stannous fluoride dentifrice are made possible by the development of a rather complicated heat application method to force stannous fluoride into solution in glycerin. That the stannous fluoride is clinically effective in such a preparation is demonstrated briefly on orthodontic patients.

ED07-0139-26

NASA Image and Video Library

2007-06-23

NASA's Ikhana unmanned science demonstration aircraft over Southern California's high desert during the ferry flight to its new home at the Dryden Flight Research Center. NASA took possession of the new aircraft in November, 2006, and it arrived at DFRC at Edwards Air Force Base, Calif., on June 23, 2007.

ED07-0139-05

NASA Image and Video Library

2007-06-23

NASA's Ikhana unmanned science demonstration aircraft over the U.S. Borax mine, Boron, California, near the Dryden/Edwards Air Force Base complex. NASA took possession of the new aircraft in November, 2006, and it arrived at the NASA Dryden Flight Research Center at Edwards AFB, Calif., on June 23, 2007.

Changes in materials properties explain the effects of humidity on gecko adhesion.

PubMed

Puthoff, Jonathan B; Prowse, Michael S; Wilkinson, Matt; Autumn, Kellar

2010-11-01

Geckos owe their remarkable stickiness to millions of dry setae on their toes, and the mechanism of adhesion in gecko setae has been the topic of scientific scrutiny for over two centuries. Previously, we demonstrated that van der Waals forces are sufficient for strong adhesion and friction in gecko setae, and that water-based capillary adhesion is not required. However, recent studies demonstrated that adhesion increases with relative humidity (RH) and proposed that surface hydration and capillary water bridge formation is important or even necessary. In this study, we confirmed a significant effect of RH on gecko adhesion, but rejected the capillary adhesion hypothesis. While contact forces of isolated tokay gecko setal arrays increased with humidity, the increase was similar on hydrophobic and hydrophilic surfaces, inconsistent with a capillary mechanism. Contact forces increased with RH even at high shear rates, where capillary bridge formation is too slow to affect adhesion. How then can a humidity-related increase in adhesion and friction be explained? The effect of RH on the mechanical properties of setal β-keratin has escaped consideration until now. We discovered that an increase in RH softens setae and increases viscoelastic damping, which increases adhesion. Changes in setal materials properties, not capillary forces, fully explain humidity-enhanced adhesion, and van der Waals forces remain the only empirically supported mechanism of adhesion in geckos.

Using the PhysX engine for physics-based virtual surgery with force feedback.

PubMed

Maciel, Anderson; Halic, Tansel; Lu, Zhonghua; Nedel, Luciana P; De, Suvranu

2009-09-01

The development of modern surgical simulators is highly challenging, as they must support complex simulation environments. The demand for higher realism in such simulators has driven researchers to adopt physics-based models, which are computationally very demanding. This poses a major problem, since real-time interactions must permit graphical updates of 30 Hz and a much higher rate of 1 kHz for force feedback (haptics). Recently several physics engines have been developed which offer multi-physics simulation capabilities, including rigid and deformable bodies, cloth and fluids. While such physics engines provide unique opportunities for the development of surgical simulators, their higher latencies, compared to what is necessary for real-time graphics and haptics, offer significant barriers to their use in interactive simulation environments. In this work, we propose solutions to this problem and demonstrate how a multimodal surgical simulation environment may be developed based on NVIDIA's PhysX physics library. Hence, models that are undergoing relatively low-frequency updates in PhysX can exist in an environment that demands much higher frequency updates for haptics. We use a collision handling layer to interface between the physical response provided by PhysX and the haptic rendering device to provide both real-time tissue response and force feedback. Our simulator integrates a bimanual haptic interface for force feedback and per-pixel shaders for graphics realism in real time. To demonstrate the effectiveness of our approach, we present the simulation of the laparoscopic adjustable gastric banding (LAGB) procedure as a case study. To develop complex and realistic surgical trainers with realistic organ geometries and tissue properties demands stable physics-based deformation methods, which are not always compatible with the interaction level required for such trainers. We have shown that combining different modelling strategies for behaviour, collision and graphics is possible and desirable. Such multimodal environments enable suitable rates to simulate the major steps of the LAGB procedure.

A socioecological analysis of the determinants of national public health nutrition work force capacity: Australia as a case study.

PubMed

Hughes, Roger

2006-01-01

This article uses a socioecological analytical approach to assess the capacity of the public health nutrition work force in Australia as a prelude to work force development strategy planning. It demonstrates how the socioecological model can be used to assess and inform the development of the infrastructure required for effective public health nutrition effort. An interpretive case study method was used involving triangular analysis of quantitative and qualitative data from multiple sources including semistructured interviews with advanced-level practitioners, literature review, a cross-sectional national work force survey, and position description audit and consensus development using a Delphi study. The findings of this analysis indicate that the Australian public health nutrition work force's capacity to effectively address priority nutrition issues is limited by determinants that can be categorized as relating to human resource infrastructure, organizational and policy environments, intelligence access and use, practice improvement and learning systems, and work force preparation. This socioecological analysis supports an intelligence-based focus for work force development effort in Australia and a conceptual framework for work force capacity assessment with potential applications in other countries.

Functional sensibility assessment. Part II: Effects of sensory improvement on precise pinch force modulation after transverse carpal tunnel release.

PubMed

Hsu, Hsiu-Yun; Kuo, Li-Chieh; Chiu, Haw-Yen; Jou, I-Ming; Su, Fong-Chin

2009-11-01

Patients with median nerve compression at the carpal tunnel often have poor sensory afferents. Without adequate sensory modulation control, these patients frequently exhibit clumsy performance and excessive force output in the affected hand. We analyzed precision grip function after the sensory recovery of patients with carpal tunnel syndrome (CTS) who underwent carpal tunnel release (CTR). Thirteen CTS patients were evaluated using a custom-designed pinch device and conventional sensory tools before and after CTR to measure sensibility, maximum pinch strength, and anticipated pinch force adjustments to movement-induced load fluctuations in a pinch-holding-up activity. Based on these tests, five force-related parameters and sensory measurements were used to determine improvements in pinch performance after sensory recovery. The force ratio between the exerted pinch force and maximum load force of the lifting object was used to determine pinch force coordination and to prove that CTR enabled precision motor output. The magnitude of peak pinch force indicated an economic force output during manipulations following CTR. The peak pinch force, force ratio, and percentage of maximum pinch force also demonstrated a moderate correlation with the Semmes-Weinstein test. Analysis of these tests revealed that improved sensory function helped restore patients' performance in precise pinch force control evaluations. These results suggest that sensory information plays an important role in adjusting balanced force output in dexterous manipulation. (c) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Studying the Frictional Force Directions via Bristles

ERIC Educational Resources Information Center

Prasitpong, S.; Chitaree, R.; Rakkapao, S.

2010-01-01

We present simple apparatus designed to help Thai high school students visualize the directions of frictional forces. Bristles of toothbrushes, paintbrushes and scrubbing brushes are used to demonstrate the frictional forces acting in a variety of situations. These demonstrations, when followed by discussion of free-body diagrams, were found to be…

Indentation quantification for in-liquid nanomechanical measurement of soft material using an atomic force microscope: rate-dependent elastic modulus of live cells.

PubMed

Ren, Juan; Yu, Shiyan; Gao, Nan; Zou, Qingze

2013-11-01

In this paper, a control-based approach to replace the conventional method to achieve accurate indentation quantification is proposed for nanomechanical measurement of live cells using atomic force microscope. Accurate indentation quantification is central to probe-based nanomechanical property measurement. The conventional method for in-liquid nanomechanical measurement of live cells, however, fails to accurately quantify the indentation as effects of the relative probe acceleration and the hydrodynamic force are not addressed. As a result, significant errors and uncertainties are induced in the nanomechanical properties measured. In this paper, a control-based approach is proposed to account for these adverse effects by tracking the same excitation force profile on both a live cell and a hard reference sample through the use of an advanced control technique, and by quantifying the indentation from the difference of the cantilever base displacement in these two measurements. The proposed control-based approach not only eliminates the relative probe acceleration effect with no need to calibrate the parameters involved, but it also reduces the hydrodynamic force effect significantly when the force load rate becomes high. We further hypothesize that, by using the proposed control-based approach, the rate-dependent elastic modulus of live human epithelial cells under different stress conditions can be reliably quantified to predict the elasticity evolution of cell membranes, and hence can be used to predict cellular behaviors. By implementing the proposed approach, the elastic modulus of HeLa cells before and after the stress process were quantified as the force load rate was changed over three orders of magnitude from 0.1 to 100 Hz, where the amplitude of the applied force and the indentation were at 0.4-2 nN and 250-450 nm, respectively. The measured elastic modulus of HeLa cells showed a clear power-law dependence on the load rate, both before and after the stress process. Moreover, the elastic modulus of HeLa cells was substantially reduced by two to five times due to the stress process. Thus, our measurements demonstrate that the control-based protocol is effective in quantifying and characterizing the evolution of nanomechanical properties during the stress process of live cells.

Daytime Cirrus Cloud Top-of-Atmosphere Radiative Forcing Properties at a Midlatitude Site and their Global Consequence

NASA Technical Reports Server (NTRS)

Campbell, James R.; Lolli, Simone; Lewis, Jasper R.; Gu, Yu; Welton, Ellsworth J.

2016-01-01

One year of continuous ground-based lidar observations (2012) is analyzed for single-layer cirrus clouds at the NASA Micro Pulse Lidar Network site at the Goddard Space Flight Center to investigate top-of-the-atmosphere (TOA) annual net daytime radiative forcing properties. A slight positive net daytime forcing is estimated (i.e., warming): 0.070.67 W m(exp -2) in sample-relative terms, which reduces to 0.030.27 W m(exp -2) in absolute terms after normalizing to unity based on a 40% midlatitude occurrence frequency rate estimated from satellite data. Results are based on bookend solutions for lidar extinction-to-backscatter (20 and 30 sr) and corresponding retrievals of the 532-nm cloud extinction coefficient. Uncertainties due to cloud under sampling, attenuation effects, sample selection, and lidar multiple scattering are described. A net daytime cooling effect is found from the very thinnest clouds (cloud optical depth of less than or equal to 0.01), which is attributed to relatively high solar zenith angles. A relationship involving positive negative daytime cloud forcing is demonstrated as a function of solar zenith angle and cloud-top temperature. These properties, combined with the influence of varying surface albedos, are used to conceptualize how daytime cloud forcing likely varies with latitude and season, with cirrus clouds exerting less positive forcing and potentially net TOA cooling approaching the summer poles (not ice and snow covered) versus greater warming at the equator. The existence of such a gradient would lead cirrus to induce varying daytime TOA forcing annually and seasonally, making it a far greater challenge than presently believed to constrain the daytime and diurnal cirrus contributions to global radiation budgets.

Atomic Force Microscopy Analysis of Nanocrystalline Patterns Fabricated Using Micromolding in Capillaries

ERIC Educational Resources Information Center

Lyman, Benjamin M.; Farmer, Orrin J.; Ramsey, Ryan D.; Lindsey, Samuel T.; Stout, Stephanie; Robison, Adam; Moore, Holly J.; Sanders, Wesley C.

2012-01-01

A cost-effective, hands-on laboratory exercise is described for demonstrating nanoscale fabrication at non-research-based educational institutions. The laboratory exercise also contains a component involving qualitative and quantitative surface characterization of student-fabricated nanoscale structures at institutions with on-site access to an…

SERDP AND NRMRL SPONSOR FIELD TEST OF COSOLVENT-ENHANCED DNAPL REMOVAL

EPA Science Inventory

A field test of multicomponent cosolvent flooding for in-situ remediation of DNAPL source zones was conducted at the Dover National Test Site (DNTS) at Dover Air Force Base, Delaware, in July, 2001. The test was part of an Enhanced Source Removal (ESR) demonstration project fund...

X-48C Hybrid - Blended Wing Body Demonstrator

NASA Image and Video Library

2013-02-28

Earth and sky met as the X-48C Hybrid Wing Body aircraft flew over Edwards Air Force Base on Feb. 28, 2013, from NASA's Dryden Flight Research Center, Edwards, CA. The long boom protruding from between the tails is part of the aircraft's parachute-deployment flight termination system.

Measurements of Aerodynamic Damping in the MIT Transonic Rotor

NASA Technical Reports Server (NTRS)

Crawley, E. F.

1981-01-01

A method was developed and demonstrated for the direct measurement of aerodynamic forcing and aerodynamic damping of a transonic compressor. The method is based on the inverse solution of the structural dynamic equations of motion of the blade disk system in order to determine the forces acting on the system. The disturbing and damping forces acting on a given blade are determined if the equations of motion are expressed in individual blade coordinates. If the structural dynamic equations are transformed to multiblade coordinates, the damping can be measured for blade disk modes, and related to a reduced frequency and interblade phase angle. In order to measure the aerodynamic damping in this way, the free response to a known excitation is studied.

Selectively transporting small chiral particles with circularly polarized Airy beams.

PubMed

Lu, Wanli; Chen, Huajin; Guo, Sandong; Liu, Shiyang; Lin, Zhifang

2018-05-01

Based on the full wave simulation, we demonstrate that a circularly polarized vector Airy beam can selectively transport small chiral particles along a curved trajectory via the chirality-tailored optical forces. The transverse optical forces can draw the chiral particles with different particle chirality towards or away from the intensity maxima of the beam, leading to the selective trapping in the transverse plane. The transversely trapped chiral particles are then accelerated along a curved trajectory of the Airy beam by the chirality-tailored longitudinal scattering force, rendering an alternative way to sort and/or transport chiral particles with specified helicity. Finally, the underlying physics of the chirality induced transverse trap and de-trap phenomena are examined by the analytical theory within the dipole approximation.

A novel experimental mechanics method for measuring the light pressure acting on a solar sail membrane

NASA Astrophysics Data System (ADS)

Shi, Aiming; Jiang, Li; Dowell, Earl H.; Qin, Zhixuan

2017-02-01

Solar sail is a high potential `sailing craft' for interstellar exploration. The area of the first flight solar sail demonstrator named "IKAROS" is 200 square meters. Future interplanetary missions will require solar sails at least on the order of 10000 square meters (or larger). Due to the limitation of ground facilities, the size of experimental sample should not be large. Furthermore the ground experiments have to be conducted in gravitational field, so the gravity effect must be considered in a ground test. To obtain insight into the solar sail membrane dynamics, a key membrane flutter (or limit cycle oscillations) experiment with light forces acting on it must be done. But one big challenge is calibrating such a tiny light force by as a function of the input power. In this paper, a gravity-based measuring method for light pressure acting on membrane is presented. To explain the experimental principle, an ideal example of a laser beam with expanders and a metal film is studied. Based on calculations, this experimental mechanics method for calibrating light pressure with an accuracy of 0.01 micro-Newton may be realized by making the light force balance the gravity force on the metal films. This gravity-based measuring method could not only be applied to study the dynamics characteristics of solar sail membrane structure with different light forces, but could also be used to determine more accurate light forces/loads acting on solar sail films and hence to enhance the determination of the mechanical properties of the solar sail membrane structure.

Development and validity of an instrumented handbike: initial results of propulsion kinetics.

PubMed

van Drongelen, Stefan; van den Berg, Jos; Arnet, Ursina; Veeger, Dirkjan H E J; van der Woude, Lucas H V

2011-11-01

To develop an instrumented handbike system to measure the forces applied to the handgrip during handbiking. A 6 degrees of freedom force sensor was built into the handgrip of an attach-unit handbike, together with two optical encoders to measure the orientation of the handgrip and crank in space. Linearity, precision, and percent error were determined for static and dynamic tests. High linearity was demonstrated for both the static and the dynamic condition (r=1.01). Precision was high under the static condition (standard deviation of 0.2N), however the precision decreased with higher loads during the dynamic condition. Percent error values were between 0.3 and 5.1%. This is the first instrumented handbike system that can register 3-dimensional forces. It can be concluded that the instrumented handbike system allows for an accurate force analysis based on forces registered at the handle bars. Copyright © 2011 IPEM. Published by Elsevier Ltd. All rights reserved.

Mechanical Properties of Single Collagen Fibrils Revealed by Force Spectroscopy

NASA Astrophysics Data System (ADS)

Graham, John; Phillips, Charlotte; Grandbois, Michel

2004-03-01

In the field of biomechanics, collagen fibrils are believed to be robust mechanical structures characterized by a low extensibility. Until very recently, information on the mechanical properties of collagen fibrils could only be derived from ensemble measurements performed on complete tissues such as bone, skin and tendon. Here we measure force-elongation/relaxation profiles of single collagen fibrils using atomic force microscopy-based force spectroscopy. The elongation profiles indicate that in vitro assembled heterotrimeric type I collagen fibrils are characterized by a large extensibility. Numerous discontinuities and a plateau in the force profile indicate major reorganization occurs within the fibrils in the 1.5 -- 4.5 nN range. Our study demonstrates that newly assembled collagen fibrils are robust structures with a significant reserve of elasticity that could play a determinant role in cellular motion in the context of tissue growth and morphogenesis. In contrast, homotrimeric collagen fibrils corresponding to osteogenesis imperfecta pathology exhibit a marked difference in their elasticity profile.

Single molecule force spectroscopy for in-situ probing oridonin inhibited ROS-mediated EGF-EGFR interactions in living KYSE-150 cells.

PubMed

Pi, Jiang; Jin, Hua; Jiang, Jinhuan; Yang, Fen; Cai, Huaihong; Yang, Peihui; Cai, Jiye; Chen, Zheng W

2017-05-01

As the active anticancer component of Rabdosia Rubescens, oridonin has been proved to show strong anticancer activity in cancer cells, which is also found to be closely related to its specific inhibition effects on the EGFR tyrosine kinase activity. In this study, atomic force microscopy based single molecule force spectroscopy (AFM-SMFS) was used for real-time and in-situ detection of EGF-EGFR interactions in living esophageal cancer KYSE-150 cells to evaluate the anticancer activity of oridonin for the first time. Oridonin was found to induce apoptosis and also reduce EGFR expression in KYSE-150 cells. AFM-SMFS results demonstrated that oridonin could inhibit the binding between EGF and EGFR in KYSE-150 cells by decreasing the unbinding force and binding probability for EGF-EGFR complexes, which was further proved to be closely associated with the intracellular ROS level. More precise mechanism studies based on AFM-SMFS demonstrated that oridonin treatment could decrease the energy barrier width, increase the dissociation off rate constant and decrease the activation energy of EGF-EGFR complexes in ROS dependent way, suggesting oridonin as a strong anticancer agent targeting EGF-EGFR interactions in cancer cells through ROS dependent mechanism. Our results not only suggested oridonin as a strong anticancer agent targeting EGF-EGFR interactions in ROS dependent mechanism, but also highlighted AFM-SMFS as a powerful technique for pharmacodynamic studies by detecting ligand-receptor interactions, which was also expected to be developed into a promising tool for the screening and mechanism studies of drugs. Copyright © 2016 Elsevier Ltd. All rights reserved.

Microbial responses to microgravity and other low-shear environments.

PubMed

Nickerson, Cheryl A; Ott, C Mark; Wilson, James W; Ramamurthy, Rajee; Pierson, Duane L

2004-06-01

Microbial adaptation to environmental stimuli is essential for survival. While several of these stimuli have been studied in detail, recent studies have demonstrated an important role for a novel environmental parameter in which microgravity and the low fluid shear dynamics associated with microgravity globally regulate microbial gene expression, physiology, and pathogenesis. In addition to analyzing fundamental questions about microbial responses to spaceflight, these studies have demonstrated important applications for microbial responses to a ground-based, low-shear stress environment similar to that encountered during spaceflight. Moreover, the low-shear growth environment sensed by microbes during microgravity of spaceflight and during ground-based microgravity analogue culture is relevant to those encountered during their natural life cycles on Earth. While no mechanism has been clearly defined to explain how the mechanical force of fluid shear transmits intracellular signals to microbial cells at the molecular level, the fact that cross talk exists between microbial signal transduction systems holds intriguing possibilities that future studies might reveal common mechanotransduction themes between these systems and those used to sense and respond to low-shear stress and changes in gravitation forces. The study of microbial mechanotransduction may identify common conserved mechanisms used by cells to perceive changes in mechanical and/or physical forces, and it has the potential to provide valuable insight for understanding mechanosensing mechanisms in higher organisms. This review summarizes recent and future research trends aimed at understanding the dynamic effects of changes in the mechanical forces that occur in microgravity and other low-shear environments on a wide variety of important microbial parameters.

Flexible Control of Safety Margins for Action Based on Environmental Variability.

PubMed

Hadjiosif, Alkis M; Smith, Maurice A

2015-06-17

To reduce the risk of slip, grip force (GF) control includes a safety margin above the force level ordinarily sufficient for the expected load force (LF) dynamics. The current view is that this safety margin is based on the expected LF dynamics, amounting to a static safety factor like that often used in engineering design. More efficient control could be achieved, however, if the motor system reduces the safety margin when LF variability is low and increases it when this variability is high. Here we show that this is indeed the case by demonstrating that the human motor system sizes the GF safety margin in proportion to an internal estimate of LF variability to maintain a fixed statistical confidence against slip. In contrast to current models of GF control that neglect the variability of LF dynamics, we demonstrate that GF is threefold more sensitive to the SD than the expected value of LF dynamics, in line with the maintenance of a 3-sigma confidence level. We then show that a computational model of GF control that includes a variability-driven safety margin predicts highly asymmetric GF adaptation between increases versus decreases in load. We find clear experimental evidence for this asymmetry and show that it explains previously reported differences in how rapidly GFs and manipulatory forces adapt. This model further predicts bizarre nonmonotonic shapes for GF learning curves, which are faithfully borne out in our experimental data. Our findings establish a new role for environmental variability in the control of action. Copyright © 2015 the authors 0270-6474/15/359106-16$15.00/0.

Microbial Responses to Microgravity and Other Low-Shear Environments

NASA Technical Reports Server (NTRS)

Nickerson, Cheryl A.; Ott, C. Mark; Wilson, James W.; Ramamurthy, Rajee; Pierson, Duane L.

2004-01-01

Microbial adaptation to environmental stimuli is essential for survival. While several of these stimuli have been studied in detail, recent studies have demonstrated an important role for a novel environmental parameter in which microgravity and the low fluid shear dynamics associated with microgravity globally regulate microbial gene expression, physiology, and pathogenesis. In addition to analyzing fundamental questions about microbial responses to spaceflight, these studies have demonstrated important applications for microbial responses to a ground-based, low-shear stress environment similar to that encountered during spaceflight. Moreover, the low-shear growth environment sensed by microbes during microgravity of spaceflight and during ground-based microgravity analogue culture is relevant to those encountered during their natural life cycles on Earth. While no mechanism has been clearly defined to explain how the mechanical force of fluid shear transmits intracellular signals to microbial cells at the molecular level, the fact that cross talk exists between microbial signal transduction systems holds intriguing possibilities that future studies might reveal common mechanotransduction themes between these systems and those used to sense and respond to low-shear stress and changes in gravitation forces. The study of microbial mechanotransduction may identify common conserved mechanisms used by cells to perceive changes in mechanical and/or physical forces, and it has the potential to provide valuable insight for understanding mechanosensing mechanisms in higher organisms. This review summarizes recent and future research trends aimed at understanding the dynamic effects of changes in the mechanical forces that occur in microgravity and other low-shear environments on a wide variety of important microbial parameters.

Hypersonic force measurements using internal balance based on optical micromachined Fabry-Perot interferometry.

PubMed

Qiu, Huacheng; Min, Fu; Zhong, Shaolong; Song, Xin; Yang, Yanguang

2018-03-01

Force measurements using wind tunnel balance are necessary for determining a variety of aerodynamic performance parameters, while the harsh environment in hypersonic flows requires that the measurement instrument should be reliable and robust, in against strong electromagnetic interference, high vacuum, or metal (oxide) dusts. In this paper, we demonstrated a three-component internal balance for hypersonic aerodynamic force measurements, using novel optical micromachined Fabry-Perot interferometric (FPI) strain gauges as sensing elements. The FPI gauges were fabricated using Micro-Opto-Electro-Mechanical Systems (MOEMS) surface and bulk fabrication techniques. High-reflectivity coatings are used to form a high-finesse Fabry-Perot cavity, which benefits a high resolution. Antireflective and passivation coatings are used to reduce unwanted interferences. The FPI strain gauge based balance has been calibrated and evaluated in a Mach 5 hypersonic flow. The results are compared with the traditional technique using the foil resistive strain gauge balance, indicating that the proposed balance based on the MOEMS FPI strain gauge is reliable and robust and is potentially suitable for the hypersonic wind tunnel harsh environment.

Hypersonic force measurements using internal balance based on optical micromachined Fabry-Perot interferometry

NASA Astrophysics Data System (ADS)

Qiu, Huacheng; Min, Fu; Zhong, Shaolong; Song, Xin; Yang, Yanguang

2018-03-01

Force measurements using wind tunnel balance are necessary for determining a variety of aerodynamic performance parameters, while the harsh environment in hypersonic flows requires that the measurement instrument should be reliable and robust, in against strong electromagnetic interference, high vacuum, or metal (oxide) dusts. In this paper, we demonstrated a three-component internal balance for hypersonic aerodynamic force measurements, using novel optical micromachined Fabry-Perot interferometric (FPI) strain gauges as sensing elements. The FPI gauges were fabricated using Micro-Opto-Electro-Mechanical Systems (MOEMS) surface and bulk fabrication techniques. High-reflectivity coatings are used to form a high-finesse Fabry-Perot cavity, which benefits a high resolution. Antireflective and passivation coatings are used to reduce unwanted interferences. The FPI strain gauge based balance has been calibrated and evaluated in a Mach 5 hypersonic flow. The results are compared with the traditional technique using the foil resistive strain gauge balance, indicating that the proposed balance based on the MOEMS FPI strain gauge is reliable and robust and is potentially suitable for the hypersonic wind tunnel harsh environment.

KSC-06pd0449

NASA Image and Video Library

2006-02-16

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers check the placement of the second half of the fairing around the Space Technology 5 (ST5) spacecraft. The ST5, which contains three microsatellites with miniaturized redundant components and technologies, is mated to its launch vehicle, Orbital Sciences' Pegasus XL. Each of the ST5 microsatellites will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. Launch of ST5 and the Pegasus XL will be from underneath the belly of an L-1011 carrier aircraft on March 14 from Vandenberg Air Force Base.

KSC-06pd0554

NASA Image and Video Library

2006-03-10

VANDENBERG AIR FORCE BASE, CALIF. - Workers prepare to transport the Space Technology 5 (ST5) spacecraft from Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California to the L-1011 carrier aircraft in position on the ramp adjacent to the Vandenberg runway. The ST5, which contains three microsatellites with miniaturized redundant components and technologies, is mated to its launch vehicle, Orbital Sciences' Pegasus XL. Each of the ST5 microsatellites will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. Launch of ST5 and the Pegasus XL will be from underneath the belly of an L-1011 carrier aircraft from Vandenberg Air Force Base.

KSC-06pd0448

NASA Image and Video Library

2006-02-16

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers position the second half of the fairing into place around the Space Technology 5 (ST5) spacecraft. The ST5, which contains three microsatellites with miniaturized redundant components and technologies, is mated to its launch vehicle, Orbital Sciences' Pegasus XL. Each of the ST5 microsatellites will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. Launch of ST5 and the Pegasus XL will be from underneath the belly of an L-1011 carrier aircraft on March 14 from Vandenberg Air Force Base.

KSC-06pd0555

NASA Image and Video Library

2006-03-10

VANDENBERG AIR FORCE BASE, CALIF. - On the ramp adjacent to the runway at Vandenberg Air Force Base in California, the Space Technology 5's Pegasus rocket is placed in position to be mated to the underside of an Orbital Sciences L-1011 carrier aircraft. The ST5, which contains three microsatellites with miniaturized redundant components and technologies, is mated to its launch vehicle, Orbital Sciences' Pegasus XL. Each of the ST5 microsatellites will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. Launch of ST5 and the Pegasus XL will be from underneath the belly of an L-1011 carrier aircraft from Vandenberg Air Force Base.

KSC-06pd0447

NASA Image and Video Library

2006-02-16

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, workers move the second half of the fairing into position around the Space Technology 5 (ST5) spacecraft. The ST5, which contains three microsatellites with miniaturized redundant components and technologies, is mated to its launch vehicle, Orbital Sciences' Pegasus XL. Each of the ST5 microsatellites will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. Launch of ST5 and the Pegasus XL will be from underneath the belly of an L-1011 carrier aircraft on March 14 from Vandenberg Air Force Base.

PDMS-based triboelectric and transparent nanogenerators with ZnO nanorod arrays.

PubMed

Ko, Yeong Hwan; Nagaraju, Goli; Lee, Soo Hyun; Yu, Jae Su

2014-05-14

Vertically-grown ZnO nanorod arrays (NRAs) on indium tin oxide (ITO)-coated polyethylene terephthalate (PET), as a top electrode of nanogenerators, were investigated for the antireflective property as well as an efficient contact surface in bare polydimethysiloxane (PDMS)-based triboelectric nanogenerators. Compared to conventional ITO-coated PET (i.e., ITO/PET), the ZnO NRAs considerably suppressed the reflectance from 20 to 9.7% at wavelengths of 300-1100 nm, creating a highly transparent top electrode, as demonstrated by theoretical analysis. Also, the interval time between the peaks of generated output voltage under external pushing forces was significantly decreased from 1.84 to 0.19 s because the reduced contact area of the PDMS by discrete surfaces of the ZnO NRAs on ITO/PET causes a rapid sequence for triboelectric charge generation process including rubbing and separating. Therefore, the use of this top electrode enabled to operate the transparent PDMS-based triboelectric nanogenerator at high frequency of external pushing force. Under different external forces of 0.3-10 kgf, the output voltage and current were also characterized.

Customization, control, and characterization of a commercial haptic device for high-fidelity rendering of weak forces.

PubMed

Gurari, Netta; Baud-Bovy, Gabriel

2014-09-30

The emergence of commercial haptic devices offers new research opportunities to enhance our understanding of the human sensory-motor system. Yet, commercial device capabilities have limitations which need to be addressed. This paper describes the customization of a commercial force feedback device for displaying forces with a precision that exceeds the human force perception threshold. The device was outfitted with a multi-axis force sensor and closed-loop controlled to improve its transparency. Additionally, two force sensing resistors were attached to the device to measure grip force. Force errors were modeled in the frequency- and time-domain to identify contributions from the mass, viscous friction, and Coulomb friction during open- and closed-loop control. The effect of user interaction on system stability was assessed in the context of a user study which aimed to measure force perceptual thresholds. Findings based on 15 participants demonstrate that the system maintains stability when rendering forces ranging from 0-0.20 N, with an average maximum absolute force error of 0.041 ± 0.013 N. Modeling the force errors revealed that Coulomb friction and inertia were the main contributors to force distortions during respectively slow and fast motions. Existing commercial force feedback devices cannot render forces with the required precision for certain testing scenarios. Building on existing robotics work, this paper shows how a device can be customized to make it reliable for studying the perception of weak forces. The customized and closed-loop controlled device is suitable for measuring force perceptual thresholds. Copyright © 2014 Elsevier B.V. All rights reserved.

The equivalent angle-of-attack method for estimating the nonlinear aerodynamic characteristics of missile wings and control surfaces

NASA Technical Reports Server (NTRS)

Hemsch, M. J.; Nielsen, J. N.

1982-01-01

A method has been developed for estimating the nonlinear aerodynamic characteristics of missile wing and control surfaces. The method is based on the following assumption: if a fin on a body has the same normal-force coefficient as a wing alone composed of two of the same fins joined together at their root chords, then the other force and moment coefficients of the fin and the wing alone are the same including the nonlinearities. The method can be used for deflected fins at arbitrary bank angles and at high angles of attack. In the paper, a full derivation of the method is given, its accuracy demonstrated and its use in extending missile data bases is shown.

Magnetic field concentration using ferromagnetic material to propel a wireless power transfer based micro-robot

NASA Astrophysics Data System (ADS)

Kim, Dongwook; Park, Bumjin; Park, Jaehyoung; Park, Hyun Ho; Ahn, Seungyoung

2018-05-01

In this paper, we propose a novel coil structure, using a ferromagnetic material which concentrates the magnetic field, as the propulsion system of a wireless power transfer (WPT) based micro-robot. This structure uses an incident magnetic field to induce current during wireless power transfer, to generate a Lorentz force. To prevent net cancelation of the Lorentz force in the load coil, ferrite films were applied to one side of the coil segment. The demonstrated simplicity and effectiveness of the proposed micro-robot showed its suitability for applications. Simulation and experimental results confirmed a velocity of 1.02 mm/s with 6 mW power transfer capacity for the 3 mm sized micro-robot.

Ultrafast single molecule technique for the study of force dependent kinetics and conformational changes of actin-protein interaction involved in mechanotransduction

NASA Astrophysics Data System (ADS)

Sergides, M.; Arbore, C.; Pavone, F. S.; Capitanio, M.

2018-02-01

Mechanical signals occurring at the interface between cell membrane and extracellular matrix and at intercellular junctions trigger biochemical signals that are fundamental for cell growth, development and regulation. Adaptor proteins, which link the cell membrane to the actin cytoskeleton, seem to partake in this process of mechanotransduction. In particular, catenins play a key role in intercellular junctions, where they act as a bridge between the cell membrane and actin. Studies suggest that α-catenin contains a domain that normally masks vinculin binding sites, which can become accessible after a conformational change induced by an external force. Here we demonstrate a single-molecule technique for investigating actin-protein interactions at different forces (up to 17 pN) with adequate temporal resolution (sub-ms). This system is based on the ultrafast force-clamp spectroscopy technique that has been recently developed by our group and is adapted to study and measure force-dependent kinetics of the catenin-actin interaction, as well as the amplitude of the expected conformational changes such as force-induced protein unfolding.

Kinetics of molecular transitions with dynamic disorder in single-molecule pulling experiments

NASA Astrophysics Data System (ADS)

Zheng, Yue; Li, Ping; Zhao, Nanrong; Hou, Zhonghuai

2013-05-01

Macromolecular transitions are subject to large fluctuations of rate constant, termed as dynamic disorder. The individual or intrinsic transition rates and activation free energies can be extracted from single-molecule pulling experiments. Here we present a theoretical framework based on a generalized Langevin equation with fractional Gaussian noise and power-law memory kernel to study the kinetics of macromolecular transitions to address the effects of dynamic disorder on barrier-crossing kinetics under external pulling force. By using the Kramers' rate theory, we have calculated the fluctuating rate constant of molecular transition, as well as the experimentally accessible quantities such as the force-dependent mean lifetime, the rupture force distribution, and the speed-dependent mean rupture force. Particular attention is paid to the discrepancies between the kinetics with and without dynamic disorder. We demonstrate that these discrepancies show strong and nontrivial dependence on the external force or the pulling speed, as well as the barrier height of the potential of mean force. Our results suggest that dynamic disorder is an important factor that should be taken into account properly in accurate interpretations of single-molecule pulling experiments.

Contact resonance atomic force microscopy for viscoelastic characterization of polymer-based nanocomposites at variable temperature

NASA Astrophysics Data System (ADS)

Natali, Marco; Passeri, Daniele; Reggente, Melania; Tamburri, Emanuela; Terranova, Maria Letizia; Rossi, Marco

2016-06-01

Characterization of mechanical properties at the nanometer scale at variable temperature is one of the main challenges in the development of polymer-based nanocomposites for application in high temperature environments. Contact resonance atomic force microscopy (CR-AFM) is a powerful technique to characterize viscoelastic properties of materials at the nanoscale. In this work, we demonstrate the capability of CR-AFM of characterizing viscoelastic properties (i.e., storage and loss moduli, as well as loss tangent) of polymer-based nanocomposites at variable temperature. CR-AFM is first illustrated on two polymeric reference samples, i.e., low-density polyethylene (LDPE) and polycarbonate (PC). Then, temperature-dependent viscoelastic properties (in terms of loss tangent) of a nanocomposite sample constituted by a epoxy resin reinforced with single-wall carbon nanotubes (SWCNTs) are investigated.

Minimising the effect of nanoparticle deformation in intermittent contact amplitude modulation atomic force microscopy measurements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Babic, Bakir, E-mail: bakir.babic@measurement.gov.au; Lawn, Malcolm A.; Coleman, Victoria A.

The results of systematic height measurements of polystyrene (PS) nanoparticles using intermittent contact amplitude modulation atomic force microscopy (IC-AM-AFM) are presented. The experimental findings demonstrate that PS nanoparticles deform during AFM imaging, as indicated by a reduction in the measured particle height. This deformation depends on the IC-AM-AFM imaging parameters, material composition, and dimensional properties of the nanoparticles. A model for nanoparticle deformation occurring during IC-AM-AFM imaging is developed as a function of the peak force which can be calculated for a particular set of experimental conditions. The undeformed nanoparticle height can be estimated from the model by extrapolation tomore » zero peak force. A procedure is proposed to quantify and minimise nanoparticle deformation during IC-AM-AFM imaging, based on appropriate adjustments of the experimental control parameters.« less

Magnetic field sensor based on the Ampere's force using dual-polarization DBR fiber laser

NASA Astrophysics Data System (ADS)

Yao, Shuang; Zhang, Yang; Guan, Baiou

2015-08-01

A novel magnetic field sensor using distributed Bragg reflector (DBR) fiber laser by Ampere's force effect is proposed and experimentally demonstrated. The key sensing element, that is the dual-polarization DBR fiber laser, is fixed on the middle part of two copper plates which carry the current. Ampere's force is applied onto the coppers due to an external magnetic field generated by a DC solenoid. Thus, the lateral force from the coppers is converted to a corresponding beat frequency signal shift produced by the DBR laser. The electric current sensing is also realized by the same configuration and same principle simultaneously in an intuitive manner. Good agreement between the theory calculation and the experimental results is obtained, which shows a good linearity. This sensor's sensitivity to the magnetic field and to the electric current finally reaches ~258.92 kHz/mT and ~1.08727 MHz/A, respectively.

Evaluation of the shear force of single cancer cells by vertically aligned carbon nanotubes suitable for metastasis diagnosis.

PubMed

Abdolahad, M; Mohajerzadeh, S; Janmaleki, M; Taghinejad, H; Taghinejad, M

2013-03-01

Vertically aligned carbon nanotube (VACNT) arrays have been demonstrated as probes for rapid quantifying of cancer cell deformability with high resolution. Through entrapment of various cancer cells on CNT arrays, the deflections of the nanotubes during cell deformation were used to derive the lateral cell shear force using a large deflection mode method. It is observed that VACNT beams act as sensitive and flexible agents, which transfer the shear force of cells trapped on them by an observable deflection. The metastatic cancer cells have significant deformable structures leading to a further cell traction force (CTF) than primary cancerous one on CNT arrays. The elasticity of different cells could be compared by their CTF measurement on CNT arrays. This study presents a nanotube-based methodology for quantifying the single cell mechanical behavior, which could be useful for understanding the metastatic behavior of cells.

Characterization of structural and electrical properties of ZnO tetrapods

NASA Astrophysics Data System (ADS)

Gu, Yu-Dong; Mai, Wen-Jie; Jiang, Peng

2011-12-01

ZnO tetrapods were synthesized by a typical thermal vapor-solid deposition method in a horizontal tube furnace. Structural characterization was carried out by transmission electron microscopy (TEM) and select-area electron diffraction (SAED), which shows the presence of zinc blende nucleus in the center of tetrapods while the four branches taking hexagonal wurtzite structure. The electrical transport property of ZnO tetrapods was investigated through an in-situ nanoprobe system. The three branches of a tetrapod serve as source, drain, and "gate", respectively; while the fourth branch pointing upward works as the force trigger by vertically applying external force downward. The conductivity of each branch of ZnO-tetrapods increases 3-4 times under pressure. In such situation, the electrical current through the branches of ZnO tetrapods can be tuned by external force, and therefore a simple force sensor based on ZnO tetrapods has been demonstrated for the first time.

Path suppression of strongly collapsing bubbles at finite and low Reynolds numbers.

PubMed

Rechiman, Ludmila M; Dellavale, Damián; Bonetto, Fabián J

2013-06-01

We study, numerically and experimentally, three different methods to suppress the trajectories of strongly collapsing and sonoluminescent bubbles in a highly viscous sulfuric acid solution. A new numerical scheme based on the window method is proposed to account for the history force acting on a spherical bubble with variable radius. We could quantify the history force, which is not negligible in comparison with the primary Bjerknes force in this type of problem, and results are in agreement with the classical primary Bjerknes force trapping threshold analysis. Moreover, the present numerical implementation reproduces the spatial behavior associated with the positional and path instability of sonoluminescent argon bubbles in strongly gassed and highly degassed sulfuric acid solutions. Finally, the model allows us to demonstrate that spatially stationary bubbles driven by biharmonic excitation could be obtained with a different mode from the one used in previous reported experiments.

Asymmetric and speed-dependent contact angle hysteresis and relaxation of a suddenly stopped moving contact line

NASA Astrophysics Data System (ADS)

Guan, Dongshi; Wang, Yong Jian; Charlaix, Elisabeth; Tong, Penger

We report direct atomic-force-microscope measurements of capillary force hysteresis and relaxation of a circular moving contact line (CL) formed on a long micron-sized hydrophobic fiber intersecting a water-air interface. The measured capillary force hysteresis and CL relaxation show a strong asymmetric speed dependence in the advancing and receding directions. A unified model based on force-assisted barrier-crossing is utilized to find the underlying energy barrier Eb and size λ associated with the defects on the fiber surface. The experiment demonstrates that the pinning (relaxation) and depinning dynamics of the CL can be described by a common microscopic frame-work, and the advancing and receding CLs are influenced by two different sets of relatively wetting and non-wetting defects on the fiber surface. Work supported in part by the Research Grants Council of Hong Kong SAR.

Optoelectrofluidic field separation based on light-intensity gradients

PubMed Central

Lee, Sanghyun; Park, Hyun Jin; Yoon, Jin Sung; Kang, Kwan Hyoung

2010-01-01

Optoelectrofluidic field separation (OEFS) of particles under light -intensity gradient (LIG) is reported, where the LIG illumination on the photoconductive layer converts the short-ranged dielectrophoresis (DEP) force to the long-ranged one. The long-ranged DEP force can compete with the hydrodynamic force by alternating current electro-osmosis (ACEO) over the entire illumination area for realizing effective field separation of particles. In the OEFS system, the codirectional illumination and observation induce the levitation effect, compensating the attenuation of the DEP force under LIG illumination by slightly floating particles from the surface. Results of the field separation and concentration of diverse particle pairs (0.82–16 μm) are well demonstrated, and conditions determining the critical radius and effective particle manipulation are discussed. The OEFS with codirectional LIG strategy could be a promising particle manipulation method in many applications where a rapid manipulation of biological cells and particles over the entire working area are of interest. PMID:20697461

Optoelectrofluidic field separation based on light-intensity gradients.

PubMed

Lee, Sanghyun; Park, Hyun Jin; Yoon, Jin Sung; Kang, Kwan Hyoung

2010-07-14

Optoelectrofluidic field separation (OEFS) of particles under light -intensity gradient (LIG) is reported, where the LIG illumination on the photoconductive layer converts the short-ranged dielectrophoresis (DEP) force to the long-ranged one. The long-ranged DEP force can compete with the hydrodynamic force by alternating current electro-osmosis (ACEO) over the entire illumination area for realizing effective field separation of particles. In the OEFS system, the codirectional illumination and observation induce the levitation effect, compensating the attenuation of the DEP force under LIG illumination by slightly floating particles from the surface. Results of the field separation and concentration of diverse particle pairs (0.82-16 mum) are well demonstrated, and conditions determining the critical radius and effective particle manipulation are discussed. The OEFS with codirectional LIG strategy could be a promising particle manipulation method in many applications where a rapid manipulation of biological cells and particles over the entire working area are of interest.

Double-Acting Sleeve Muscle Actuator for Bio-Robotic Systems.

PubMed

Zheng, Hao; Shen, Xiangrong

2013-11-25

This paper presents a new type of muscle-like actuator, namely double-acting (DA) sleeve muscle actuator, which is suitable for the actuation of biologically-inspired and biomedical robotic systems, especially those serving human-assistance purposes (prostheses, orthoses, etc .). Developed based on the traditional pneumatic muscle actuator, the new DA sleeve muscle incorporates a unique insert at the center. With the insert occupying the central portion of the internal volume, this new actuator enjoys multiple advantages relative to the traditional pneumatic muscle, including a consistent increase of force capacity over the entire range of motion, and a significant decrease of energy consumption in operation. Furthermore, the insert encompasses an additional chamber, which generates an extension force when pressurized. As such, this new actuator provides a unique bi-directional actuation capability, and, thus, has a potential to significantly simplify the design of a muscle actuator-powered robotic system. To demonstrate this new actuator concept, a prototype has been designed and fabricated, and experiments conducted on this prototype demonstrated the enhanced force capacity and the unique bi-directional actuation capability.

Floating electrode dielectrophoresis.

PubMed

Golan, Saar; Elata, David; Orenstein, Meir; Dinnar, Uri

2006-12-01

In practice, dielectrophoresis (DEP) devices are based on micropatterned electrodes. When subjected to applied voltages, the electrodes generate nonuniform electric fields that are necessary for the DEP manipulation of particles. In this study, electrically floating electrodes are used in DEP devices. It is demonstrated that effective DEP forces can be achieved by using floating electrodes. Additionally, DEP forces generated by floating electrodes are different from DEP forces generated by excited electrodes. The floating electrodes' capabilities are explained theoretically by calculating the electric field gradients and demonstrated experimentally by using test-devices. The test-devices show that floating electrodes can be used to collect erythrocytes (red blood cells). DEP devices which contain many floating electrodes ought to have fewer connections to external signal sources. Therefore, the use of floating electrodes may considerably facilitate the fabrication and operation of DEP devices. It can also reduce device dimensions. However, the key point is that DEP devices can integrate excited electrodes fabricated by microtechnology processes and floating electrodes fabricated by nanotechnology processes. Such integration is expected to promote the use of DEP devices in the manipulation of nanoparticles.

Dystrophin restoration therapy improves both the reduced excitability and the force drop induced by lengthening contractions in dystrophic mdx skeletal muscle.

PubMed

Roy, Pauline; Rau, Fredérique; Ochala, Julien; Messéant, Julien; Fraysse, Bodvael; Lainé, Jeanne; Agbulut, Onnik; Butler-Browne, Gillian; Furling, Denis; Ferry, Arnaud

2016-01-01

The greater susceptibility to contraction-induced skeletal muscle injury (fragility) is an important dystrophic feature and tool for testing preclinic dystrophin-based therapies for Duchenne muscular dystrophy. However, how these therapies reduce the muscle fragility is not clear. To address this question, we first determined the event(s) of the excitation-contraction cycle which is/are altered following lengthening (eccentric) contractions in the mdx muscle. We found that the immediate force drop following lengthening contractions, a widely used measure of muscle fragility, was associated with reduced muscle excitability. Moreover, the force drop can be mimicked by an experimental reduction in muscle excitation of uninjured muscle. Furthermore, the force drop was not related to major neuromuscular transmission failure, excitation-contraction uncoupling, and myofibrillar impairment. Secondly, and importantly, the re-expression of functional truncated dystrophin in the muscle of mdx mice using an exon skipping strategy partially prevented the reductions in both force drop and muscle excitability following lengthening contractions. We demonstrated for the first time that (i) the increased susceptibility to contraction-induced muscle injury in mdx mice is mainly attributable to reduced muscle excitability; (ii) dystrophin-based therapy improves fragility of the dystrophic skeletal muscle by preventing reduction in muscle excitability.

Development and Performance Evaluation of Image-Based Robotic Waxing System for Detailing Automobiles

PubMed Central

Hsu, Bing-Cheng

2018-01-01

Waxing is an important aspect of automobile detailing, aimed at protecting the finish of the car and preventing rust. At present, this delicate work is conducted manually due to the need for iterative adjustments to achieve acceptable quality. This paper presents a robotic waxing system in which surface images are used to evaluate the quality of the finish. An RGB-D camera is used to build a point cloud that details the sheet metal components to enable path planning for a robot manipulator. The robot is equipped with a multi-axis force sensor to measure and control the forces involved in the application and buffing of wax. Images of sheet metal components that were waxed by experienced car detailers were analyzed using image processing algorithms. A Gaussian distribution function and its parameterized values were obtained from the images for use as a performance criterion in evaluating the quality of surfaces prepared by the robotic waxing system. Waxing force and dwell time were optimized using a mathematical model based on the image-based criterion used to measure waxing performance. Experimental results demonstrate the feasibility of the proposed robotic waxing system and image-based performance evaluation scheme. PMID:29757940

Development and Performance Evaluation of Image-Based Robotic Waxing System for Detailing Automobiles.

PubMed

Lin, Chi-Ying; Hsu, Bing-Cheng

2018-05-14

Waxing is an important aspect of automobile detailing, aimed at protecting the finish of the car and preventing rust. At present, this delicate work is conducted manually due to the need for iterative adjustments to achieve acceptable quality. This paper presents a robotic waxing system in which surface images are used to evaluate the quality of the finish. An RGB-D camera is used to build a point cloud that details the sheet metal components to enable path planning for a robot manipulator. The robot is equipped with a multi-axis force sensor to measure and control the forces involved in the application and buffing of wax. Images of sheet metal components that were waxed by experienced car detailers were analyzed using image processing algorithms. A Gaussian distribution function and its parameterized values were obtained from the images for use as a performance criterion in evaluating the quality of surfaces prepared by the robotic waxing system. Waxing force and dwell time were optimized using a mathematical model based on the image-based criterion used to measure waxing performance. Experimental results demonstrate the feasibility of the proposed robotic waxing system and image-based performance evaluation scheme.

KSC-06pd0166

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — n the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, a third satellite is transported across the floor. It will be mounted with the other satellites on the payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0165

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers lower the second satellite onto the payload support structure. Three micro-satellites are being mounted on a payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

KSC-06pd0432

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, this photo shows two of the Space Technology 5 (ST5) spacecraft's microsatellites mounted on the payload structure that is mated to the Orbital Sciences' Pegasus XL launch vehicle. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0433

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. - Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California, this closeup shows two of the Space Technology 5 (ST5) spacecraft's microsatellites mounted on the payload structure that is mated to the Orbital Sciences' Pegasus XL launch vehicle. In the background is the fairing that will enclose the ST5 for launch. The ST5 contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0436

NASA Image and Video Library

2006-02-14

VANDENBERG AIR FORCE BASE, CALIF. -Inside Orbital Sciences’ Building 1555 at Vandenberg Air Force Base in California is the Pegasus XL launch vehicle and the Space Technology 5 (ST5) spacecraft being prepared for encapsulation before launch. The ST5, mated to Orbital Sciences' Pegasus XL launch vehicle, contains three microsatellites with miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled from the belly of an L-1011 carrier aircraft no earlier than March 14 from Vandenberg Air Force Base.

KSC-06pd0164

NASA Image and Video Library

2006-01-12

VANDENBERG AIR FORCE BASE, Calif. — In the Orbital Sciences Building 836 at Vandenberg Air Force Base in California, workers are maneuvering a second satellite suspended by an overhead crane. Three micro-satellites are being mounted on a payload support structure. The three satellites make up the Space Technology 5 spacecraft, called ST5, and will be launched by a Pegasus XL rocket. The satellites contain miniaturized redundant components and technologies. Each will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. With such missions, NASA hopes to improve scientists’ ability to accurately forecast space weather and minimize its harmful effects on space- and ground-based systems. Launch of ST5 is scheduled for Feb. 28 from Vandenberg Air Force Base.

A self-assembled nanoscale robotic arm controlled by electric fields

NASA Astrophysics Data System (ADS)

Kopperger, Enzo; List, Jonathan; Madhira, Sushi; Rothfischer, Florian; Lamb, Don C.; Simmel, Friedrich C.

2018-01-01

The use of dynamic, self-assembled DNA nanostructures in the context of nanorobotics requires fast and reliable actuation mechanisms. We therefore created a 55-nanometer–by–55-nanometer DNA-based molecular platform with an integrated robotic arm of length 25 nanometers, which can be extended to more than 400 nanometers and actuated with externally applied electrical fields. Precise, computer-controlled switching of the arm between arbitrary positions on the platform can be achieved within milliseconds, as demonstrated with single-pair Förster resonance energy transfer experiments and fluorescence microscopy. The arm can be used for electrically driven transport of molecules or nanoparticles over tens of nanometers, which is useful for the control of photonic and plasmonic processes. Application of piconewton forces by the robot arm is demonstrated in force-induced DNA duplex melting experiments.

Programmed folding of DNA origami structures through single-molecule force control.

PubMed

Bae, Wooli; Kim, Kipom; Min, Duyoung; Ryu, Je-Kyung; Hyeon, Changbong; Yoon, Tae-Young

2014-12-03

Despite the recent development in the design of DNA origami, its folding yet relies on thermal or chemical annealing methods. We here demonstrate mechanical folding of the DNA origami structure via a pathway that has not been accessible to thermal annealing. Using magnetic tweezers, we stretch a single scaffold DNA with mechanical tension to remove its secondary structures, followed by base pairing of the stretched DNA with staple strands. When the force is subsequently quenched, folding of the DNA nanostructure is completed through displacement between the bound staple strands. Each process in the mechanical folding is well defined and free from kinetic traps, enabling us to complete folding within 10 min. We also demonstrate parallel folding of DNA nanostructures through multiplexed manipulation of the scaffold DNAs. Our results suggest a path towards programmability of the folding pathway of DNA nanostructures.

Border Forces and Friction Control Epithelial Closure Dynamics

PubMed Central

Cochet-Escartin, Olivier; Ranft, Jonas; Silberzan, Pascal; Marcq, Philippe

2014-01-01

We study the closure dynamics of a large number of well-controlled circular apertures within an epithelial monolayer, where the collective cell migration responsible for epithelization is triggered by the removal of a spatial constraint rather than by scratching. Based on experimental observations, we propose a physical model that takes into account border forces, friction with the substrate, and tissue rheology. Border protrusive activity drives epithelization despite the presence of a contractile actomyosin cable at the periphery of the wound. The closure dynamics is quantified by an epithelization coefficient, defined as the ratio of protrusive stress to tissue-substrate friction, that allows classification of different phenotypes. The same analysis demonstrates a distinct signature for human cells bearing the oncogenic RasV12 mutation, demonstrating the potential of the approach to quantitatively characterize metastatic transformations. PMID:24411238

Fibrin matrices with affinity-based delivery systems and neurotrophic factors promote functional nerve regeneration.

PubMed

Wood, Matthew D; MacEwan, Matthew R; French, Alexander R; Moore, Amy M; Hunter, Daniel A; Mackinnon, Susan E; Moran, Daniel W; Borschel, Gregory H; Sakiyama-Elbert, Shelly E

2010-08-15

Glial-derived neurotrophic factor (GDNF) and nerve growth factor (NGF) have both been shown to enhance peripheral nerve regeneration following injury and target different neuronal populations. The delivery of either growth factor at the site of injury may, therefore, result in quantitative differences in motor nerve regeneration and functional recovery. In this study we evaluated the effect of affinity-based delivery of GDNF or NGF from fibrin-filled nerve guidance conduits (NGCs) on motor nerve regeneration and functional recovery in a 13 mm rat sciatic nerve defect. Seven experimental groups were evaluated consisting of GDNF or NGF and the affinity-based delivery system (DS) within NGCs, control groups excluding the DS and/or growth factor, and nerve isografts. Groups with growth factor in the conduit demonstrated equivalent or superior performance in behavioral tests and relative muscle mass measurements compared to isografts at 12 weeks. Additionally, groups with GDNF demonstrated greater specific twitch and tetanic force production in extensor digitorum longus (EDL) muscle than the isograft control, while groups with NGF produced demonstrated similar force production compared to the isograft control. Assessment of motor axon regeneration by retrograde labeling further revealed that the number of ventral horn neurons regenerating across NGCs containing GDNF and NGF DS was similar to the isograft group and these counts were greater than the groups without growth factor. Overall, the GDNF DS group demonstrated superior functional recovery and equivalent motor nerve regeneration compared to the isograft control, suggesting it has potential as a treatment for motor nerve injury.

Demonstrating Circular Motion with a Model Satellite/Earth System

ERIC Educational Resources Information Center

Whittaker, Jeff

2008-01-01

A number of interesting demonstrations of circular and satellite motion have been described in this journal. This paper presents a variation of a centripetal force apparatus found in G.D. Freier and F.J. Anderson's "A Demonstration Handbook for Physics," which has been modified in order to demonstrate both centripetal force and satellite motion.…

Rehabilitation of the Rocket Vehicle Integration Test Stand at Edwards Air Force Base

NASA Technical Reports Server (NTRS)

Jones, Daniel S.; Ray, Ronald J.; Phillips, Paul

2005-01-01

Since initial use in 1958 for the X-15 rocket-powered research airplane, the Rocket Engine Test Facility has proven essential for testing and servicing rocket-powered vehicles at Edwards Air Force Base. For almost two decades, several successful flight-test programs utilized the capability of this facility. The Department of Defense has recently demonstrated a renewed interest in propulsion technology development with the establishment of the National Aerospace Initiative. More recently, the National Aeronautics and Space Administration is undergoing a transformation to realign the organization, focusing on the Vision for Space Exploration. These initiatives provide a clear indication that a very capable ground-test stand at Edwards Air Force Base will be beneficial to support the testing of future access-to-space vehicles. To meet the demand of full integration testing of rocket-powered vehicles, the NASA Dryden Flight Research Center, the Air Force Flight Test Center, and the Air Force Research Laboratory have combined their resources in an effort to restore and upgrade the original X-15 Rocket Engine Test Facility to become the new Rocket Vehicle Integration Test Stand. This report describes the history of the X-15 Rocket Engine Test Facility, discusses the current status of the facility, and summarizes recent efforts to rehabilitate the facility to support potential access-to-space flight-test programs. A summary of the capabilities of the facility is presented and other important issues are discussed.

Finite element analysis of the effect of force directions on tooth movement in extraction space closure with miniscrew sliding mechanics.

PubMed

Kojima, Yukio; Kawamura, Jun; Fukui, Hisao

2012-10-01

Miniscrews placed in bone have been used as orthodontic anchorage in extraction space closure with sliding mechanics. The movement patterns of the teeth depend on the force directions. To move the teeth in a desired pattern, the appropriate direction of force must be selected. The purpose of this article is to clarify the relationship between force directions and movement patterns. By using the finite element method, orthodontic movements were simulated based on the remodeling law of the alveolar bone. The power arm length and the miniscrew position were varied to change the force directions. When the power arm was lengthened, rotation of the entire maxillary dentition decreased. The posterior teeth were effective for preventing rotation of the anterior teeth through an archwire. In cases of a high position of a miniscrew, bodily tooth movement was almost achieved. The vertical component of the force produced intrusion or extrusion of the entire dentition. Within the limits of the method, the mechanical simulations demonstrated the effect of force direction on movement patterns. Copyright © 2012 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.

Wear-dependent specific coefficients in a mechanistic model for turning of nickel-based superalloy with ceramic tools

NASA Astrophysics Data System (ADS)

López de Lacalle, Luis Norberto; Urbicain Pelayo, Gorka; Fernández-Valdivielso, Asier; Alvarez, Alvaro; González, Haizea

2017-09-01

Difficult to cut materials such as nickel and titanium alloys are used in the aeronautical industry, the former alloys due to its heat-resistant behavior and the latter for the low weight - high strength ratio. Ceramic tools made out alumina with reinforce SiC whiskers are a choice in turning for roughing and semifinishing workpiece stages. Wear rate is high in the machining of these alloys, and consequently cutting forces tends to increase along one operation. This paper establishes the cutting force relation between work-piece and tool in the turning of such difficult-to-cut alloys by means of a mechanistic cutting force model that considers the tool wear effect. The cutting force model demonstrates the force sensitivity to the cutting engagement parameters (ap, f) when using ceramic inserts and wear is considered. Wear is introduced through a cutting time factor, being useful in real conditions taking into account that wear quickly appears in alloys machining. A good accuracy in the cutting force model coefficients is the key issue for an accurate prediction of turning forces, which could be used as criteria for tool replacement or as input for chatter or other models.

Blue guardian: an open architecture for rapid ISR demonstration

NASA Astrophysics Data System (ADS)

Barrett, Donald A.; Borntrager, Luke A.; Green, David M.

2016-05-01

Throughout the Department of Defense (DoD), acquisition, platform integration, and life cycle costs for weapons systems have continued to rise. Although Open Architecture (OA) interface standards are one of the primary methods being used to reduce these costs, the Air Force Rapid Capabilities Office (AFRCO) has extended the OA concept and chartered the Open Mission System (OMS) initiative with industry to develop and demonstrate a consensus-based, non-proprietary, OA standard for integrating subsystems and services into airborne platforms. The new OMS standard provides the capability to decouple vendor-specific sensors, payloads, and service implementations from platform-specific architectures and is still in the early stages of maturation and demonstration. The Air Force Research Laboratory (AFRL) - Sensors Directorate has developed the Blue Guardian program to demonstrate advanced sensing technology utilizing open architectures in operationally relevant environments. Over the past year, Blue Guardian has developed a platform architecture using the Air Force's OMS reference architecture and conducted a ground and flight test program of multiple payload combinations. Systems tested included a vendor-unique variety of Full Motion Video (FMV) systems, a Wide Area Motion Imagery (WAMI) system, a multi-mode radar system, processing and database functions, multiple decompression algorithms, multiple communications systems, and a suite of software tools. Initial results of the Blue Guardian program show the promise of OA to DoD acquisitions, especially for Intelligence, Surveillance and Reconnaissance (ISR) payload applications. Specifically, the OMS reference architecture was extremely useful in reducing the cost and time required for integrating new systems.

A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers.

PubMed

Kawamura, Ryuzo; Miyazaki, Minami; Shimizu, Keita; Matsumoto, Yuta; Silberberg, Yaron R; Sathuluri, Ramachandra Rao; Iijima, Masumi; Kuroda, Shun'ichi; Iwata, Futoshi; Kobayashi, Takeshi; Nakamura, Chikashi

2017-11-08

Focusing on intracellular targets, we propose a new cell separation technique based on a nanoneedle array (NNA) device, which allows simultaneous insertion of multiple needles into multiple cells. The device is designed to target and lift ("fish") individual cells from a mixed population of cells on a substrate using an antibody-functionalized NNA. The mechanics underlying this approach were validated by force analysis using an atomic force microscope. Accurate high-throughput separation was achieved using one-to-one contacts between the nanoneedles and the cells by preparing a single-cell array in which the positions of the cells were aligned with 10,000 nanoneedles in the NNA. Cell-type-specific separation was realized by controlling the adhesion force so that the cells could be detached in cell-type-independent manner. Separation of nestin-expressing neural stem cells (NSCs) derived from human induced pluripotent stem cells (hiPSCs) was demonstrated using the proposed technology, and successful differentiation to neuronal cells was confirmed.

Consistency between satellite-derived and modeled estimates of the direct aerosol effect.

PubMed

Myhre, Gunnar

2009-07-10

In the Intergovernmental Panel on Climate Change Fourth Assessment Report, the direct aerosol effect is reported to have a radiative forcing estimate of -0.5 Watt per square meter (W m(-2)), offsetting the warming from CO2 by almost one-third. The uncertainty, however, ranges from -0.9 to -0.1 W m(-2), which is largely due to differences between estimates from global aerosol models and observation-based estimates, with the latter tending to have stronger (more negative) radiative forcing. This study demonstrates consistency between a global aerosol model and adjustment to an observation-based method, producing a global and annual mean radiative forcing that is weaker than -0.5 W m(-2), with a best estimate of -0.3 W m(-2). The physical explanation for the earlier discrepancy is that the relative increase in anthropogenic black carbon (absorbing aerosols) is much larger than the overall increase in the anthropogenic abundance of aerosols.

Finite-time adaptive sliding mode force control for electro-hydraulic load simulator based on improved GMS friction model

NASA Astrophysics Data System (ADS)

Kang, Shuo; Yan, Hao; Dong, Lijing; Li, Changchun

2018-03-01

This paper addresses the force tracking problem of electro-hydraulic load simulator under the influence of nonlinear friction and uncertain disturbance. A nonlinear system model combined with the improved generalized Maxwell-slip (GMS) friction model is firstly derived to describe the characteristics of load simulator system more accurately. Then, by using particle swarm optimization (PSO) algorithm ​combined with the system hysteresis characteristic analysis, the GMS friction parameters are identified. To compensate for nonlinear friction and uncertain disturbance, a finite-time adaptive sliding mode control method is proposed based on the accurate system model. This controller has the ability to ensure that the system state moves along the nonlinear sliding surface to steady state in a short time as well as good dynamic properties under the influence of parametric uncertainties and disturbance, which further improves the force loading accuracy and rapidity. At the end of this work, simulation and experimental results are employed to demonstrate the effectiveness of the proposed sliding mode control strategy.

Effect of angular velocity on sensors based on morphology dependent resonances.

PubMed

Ali, Amir R; Ioppolo, Tindaro

2014-04-22

We carried out an analysis to investigate the morphology dependent optical resonances shift (MDR) of a rotating spherical resonator. The spinning resonator experiences an elastic deformation due to the centrifugal force acting on it, leading to a shift in its MDR. Experiments are also carried out to demonstrate the MDR shifts of a spinning polydimethylsiloxane (PDMS) microsphere. The experimental results agree well with the analytical prediction. These studies demonstrated that spinning sensor based on MDR may experience sufficient shift in the optical resonances, therefore interfering with its desirable operational sensor design. Also the results show that angular velocity sensors could be designed using this principle.

Landau instability and mobility edges of the interacting one-dimensional Bose gas in weak random potentials

NASA Astrophysics Data System (ADS)

Cherny, Alexander Yu; Caux, Jean-Sébastien; Brand, Joachim

2018-01-01

We study the frictional force exerted on the trapped, interacting 1D Bose gas under the influence of a moving random potential. Specifically we consider weak potentials generated by optical speckle patterns with finite correlation length. We show that repulsive interactions between bosons lead to a superfluid response and suppression of frictional force, which can inhibit the onset of Anderson localisation. We perform a quantitative analysis of the Landau instability based on the dynamic structure factor of the integrable Lieb-Liniger model and demonstrate the existence of effective mobility edges.

Limit cycles of a flexible shaft with hydrodynamic journal bearings in unstable regimes

NASA Technical Reports Server (NTRS)

Brown, R. D.; Black, H. F.

1980-01-01

A symmetric 3 mass rotor supported on hydrodynamic bearings is described. An approximate method of representing finite bearings is used to calculate bearing forces. As the method sums forces from a number of independent circular lobes lemon 3 and 4 lobe bearings are taken into account. The calculations are based on an axial groove bearing. Linear analysis precedes nonlinear simulation of some unstable conditions. The demonstration of small limit cycles suggests that necessarily flexible rotors e.g., helicopter tail rotors, may be practical without either tilt pad bearings or external dampers.

Report for the MPV Demonstration at New Boston Air Force Base, New Hampshire: UXO Characterization in Challenging Survey Environments Using the MPV

DTIC Science & Technology

2017-04-28

EM Electromagnetic EMI Electromagnetic Induction ERDC Engineering Research and Development Center ESTCP Environmental Security Technology... bombs , HE bombs and incendiary bombs . The site hosted demonstrations with the MPV and the 2x2 TEMTADS, both with crews from CH2MHill. Each study...array of five receiver units that measure all three components of the EM field (Figure 2). This second-generation MPV is specifically designed to

Unobtrusive Estimation of Cardiac Contractility and Stroke Volume Changes Using Ballistocardiogram Measurements on a High Bandwidth Force Plate

PubMed Central

Ashouri, Hazar; Orlandic, Lara; Inan, Omer T.

2016-01-01

Unobtrusive and inexpensive technologies for monitoring the cardiovascular health of heart failure (HF) patients outside the clinic can potentially improve their continuity of care by enabling therapies to be adjusted dynamically based on the changing needs of the patients. Specifically, cardiac contractility and stroke volume (SV) are two key aspects of cardiovascular health that change significantly for HF patients as their condition worsens, yet these parameters are typically measured only in hospital/clinical settings, or with implantable sensors. In this work, we demonstrate accurate measurement of cardiac contractility (based on pre-ejection period, PEP, timings) and SV changes in subjects using ballistocardiogram (BCG) signals detected via a high bandwidth force plate. The measurement is unobtrusive, as it simply requires the subject to stand still on the force plate while holding electrodes in the hands for simultaneous electrocardiogram (ECG) detection. Specifically, we aimed to assess whether the high bandwidth force plate can provide accuracy beyond what is achieved using modified weighing scales we have developed in prior studies, based on timing intervals, as well as signal-to-noise ratio (SNR) estimates. Our results indicate that the force plate BCG measurement provides more accurate timing information and allows for better estimation of PEP than the scale BCG (r2 = 0.85 vs. r2 = 0.81) during resting conditions. This correlation is stronger during recovery after exercise due to more significant changes in PEP (r2 = 0.92). The improvement in accuracy can be attributed to the wider bandwidth of the force plate. ∆SV (i.e., changes in stroke volume) estimations from the force plate BCG resulted in an average error percentage of 5.3% with a standard deviation of ±4.2% across all subjects. Finally, SNR calculations showed slightly better SNR in the force plate measurements among all subjects but the small difference confirmed that SNR is limited by motion artifacts rather than instrumentation. PMID:27240380

Credit WCT. Original 2'" x 2'" color negative is housed ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Credit WCT. Original 2-'" x 2-'" color negative is housed in the JPL Photography Laboratory, Pasadena, California. View shows small autoclave demonstrated by JPL staff member Milton Clay (JPL negative no. JPL-10286AC, 27 January 1989). - Jet Propulsion Laboratory Edwards Facility, Liner Laboratory, Edwards Air Force Base, Boron, Kern County, CA

Further Education and Training: A Comparison of Policy Models in Britain and Norway.

ERIC Educational Resources Information Center

Skinningsrud, Tone

1995-01-01

Compares public intervention schemes in Britain and Norway supporting participation of public educational institutions in the delivery of continuing labor force development and training. These schemes demonstrate that British policy is based on belief in free market principles, while Norwegian policy combines elements of consumer choice and legal…

Piezoelectric Pre-Stressed Bending Mechanism for Impact-Driven Energy Harvester

NASA Astrophysics Data System (ADS)

Abdal, A. M.; Leong, K. S.

2017-06-01

This paper experimentally demonstrates and evaluates a piezoelectric power generator bending mechanism based on pre-stressed condition whereby the piezoelectric transducer being bended and remained in the stressed condition before applying a force on the piezoelectric bending structure, which increase the stress on the piezoelectric surface and hence increase the generated electrical charges. An impact force is being exerted onto bending the piezoelectric beam and hence generating electrical power across an external resistive load. The proposed bending mechanism prototype has been manufactured by employing 3D printer technology in order to conduct the evaluation. A free fall test has been conducted as the evaluation method with varying force using a series of different masses and different fall heights. A rectangular piezoelectric harvester beam with the size of 32mm in width, 70mm in length, and 0.55mm in thickness is used to demonstrate the experiment. It can be seen from the experiment that the instantaneous peak to peak AC volt output measured at open-circuit is increasing and saturated at about of 70V when an impact force of about 80N is being applied. It is also found that a maximum power of about 53mW is generated at an impact force of 50N when it is connected to an external resistive load of 0.7KΩ. The reported mechanism is a promising candidate in the application of energy harvesting for powering various wireless sensor nodes (WSN) which is the core of Internet of Things (IoT).

A method for estimating the mass properties of a manipulator by measuring the reaction moments at its base

NASA Technical Reports Server (NTRS)

West, Harry; Papadopoulos, Evangelos; Dubowsky, Steven; Cheah, Hanson

1989-01-01

Emulating on earth the weightlessness of a manipulator floating in space requires knowledge of the manipulator's mass properties. A method for calculating these properties by measuring the reaction forces and moments at the base of the manipulator is described. A manipulator is mounted on a 6-DOF sensor, and the reaction forces and moments at its base are measured for different positions of the links as well as for different orientations of its base. A procedure is developed to calculate from these measurements some combinations of the mass properties. The mass properties identified are not sufficiently complete for computed torque and other dynamic control techniques, but do allow compensation for the gravitational load on the links, and for simulation of weightless conditions on a space emulator. The algorithm has been experimentally demonstrated on a PUMA 260 and used to measure the independent combinations of the 16 mass parameters of the base and three proximal links.

Effect of wire size on maxillary arch force/couple systems for a simulated high canine malocclusion.

PubMed

Major, Paul W; Toogood, Roger W; Badawi, Hisham M; Carey, Jason P; Seru, Surbhi

2014-12-01

To better understand the effects of copper nickel titanium (CuNiTi) archwire size on bracket-archwire mechanics through the analysis of force/couple distributions along the maxillary arch. The hypothesis is that wire size is linearly related to the forces and moments produced along the arch. An Orthodontic Simulator was utilized to study a simplified high canine malocclusion. Force/couple distributions produced by passive and elastic ligation using two wire sizes (Damon 0.014 and 0.018 inch) measured with a sample size of 144. The distribution and variation in force/couple loading around the arch is a complicated function of wire size. The use of a thicker wire increases the force/couple magnitudes regardless of ligation method. Owing to the non-linear material behaviour of CuNiTi, this increase is less than would occur based on linear theory as would apply for stainless steel wires. The results demonstrate that an increase in wire size does not result in a proportional increase of applied force/moment. This discrepancy is explained in terms of the non-linear properties of CuNiTi wires. This non-proportional force response in relation to increased wire size warrants careful consideration when selecting wires in a clinical setting. © 2014 British Orthodontic Society.

Step-to-step spatiotemporal variables and ground reaction forces of intra-individual fastest sprinting in a single session.

PubMed

Nagahara, Ryu; Mizutani, Mirai; Matsuo, Akifumi; Kanehisa, Hiroaki; Fukunaga, Tetsuo

2018-06-01

We aimed to investigate the step-to-step spatiotemporal variables and ground reaction forces during the acceleration phase for characterising intra-individual fastest sprinting within a single session. Step-to-step spatiotemporal variables and ground reaction forces produced by 15 male athletes were measured over a 50-m distance during repeated (three to five) 60-m sprints using a long force platform system. Differences in measured variables between the fastest and slowest trials were examined at each step until the 22nd step using a magnitude-based inferences approach. There were possibly-most likely higher running speed and step frequency (2nd to 22nd steps) and shorter support time (all steps) in the fastest trial than in the slowest trial. Moreover, for the fastest trial there were likely-very likely greater mean propulsive force during the initial four steps and possibly-very likely larger mean net anterior-posterior force until the 17th step. The current results demonstrate that better sprinting performance within a single session is probably achieved by 1) a high step frequency (except the initial step) with short support time at all steps, 2) exerting a greater mean propulsive force during initial acceleration, and 3) producing a greater mean net anterior-posterior force during initial and middle acceleration.

Centrifuges in gravitational physiology research

NASA Technical Reports Server (NTRS)

Ballard, Rodney W.; Davies, Phil; Fuller, Charles A.

1993-01-01

Data from space flight and ground based experiments have clearly demonstrated the importance of Earth gravity for normal physiological function in man and animals. Gravitational Physiology is concerned with the role and influence of gravity on physiological systems. Research in this field examines how we perceive and respond to gravity and the mechanisms underlying these responses. Inherent in our search for answers to these questions is the ability to alter gravity, which is not physically possible without leaving Earth. However, useful experimental paradigms have been to modify the perceived force of gravity by changing either the orientation of subjects to the gravity vector (i.e., postural changes) or by applying inertial forces to augment the magnitude of the gravity vector. The later technique has commonly been used by applying centripetal force via centrifugation.

Current-induced changes of migration energy barriers in graphene and carbon nanotubes.

PubMed

Obodo, J T; Rungger, I; Sanvito, S; Schwingenschlögl, U

2016-05-21

An electron current can move atoms in a nanoscale device with important consequences for the device operation and breakdown. We perform first principles calculations aimed at evaluating the possibility of changing the energy barriers for atom migration in carbon-based systems. In particular, we consider the migration of adatoms and defects in graphene and carbon nanotubes. Although the current-induced forces are large for both the systems, in graphene the force component along the migration path is small and therefore the barrier height is little affected by the current flow. In contrast, the same barrier is significantly reduced in carbon nanotubes as the current increases. Our work also provides a real-system numerical demonstration that current-induced forces within density functional theory are non-conservative.

Electrostatic demonstration of free-fall weightlessness

NASA Astrophysics Data System (ADS)

Balukovic, Jasmina; Slisko, Josip; Corona Cruz, Adrian

2015-05-01

The phenomena of free-fall weightlessness have been demonstrated to students for many years in a number of different ways. The essential basis of all these demonstrations is the fact that in free-falling, gravitationally accelerated systems, the weight force and weight-related forces (for example, friction and hydrostatic forces) disappear. In this article, an original electrostatic demonstration of weightlessness is presented. A charged balloon fixed at the opening of a plastic container cannot lift a light styrofoam sphere sitting on the bottom when the container is at rest. However, while the system is in free-fall, the sphere becomes weightless and the charged balloon is able to lift it electrostatically.

Contact Forces between Single Metal Oxide Nanoparticles in Gas-Phase Applications and Processes.

PubMed

Salameh, Samir; van der Veen, Monique A; Kappl, Michael; van Ommen, J Ruud

2017-03-14

In this work we present a comprehensive experimental study to determine the contact forces between individual metal oxide nanoparticles in the gas-phase using atomic force microscopy. In addition, we determined the amount of physisorbed water for each type of particle surface. By comparing our results with mathematical models of the interaction forces, we could demonstrate that classical continuum models of van der Waals and capillary forces alone cannot sufficiently describe the experimental findings. Rather, the discrete nature of the molecules has to be considered, which leads to ordering at the interface and the occurrence of solvation forces. We demonstrate that inclusion of solvation forces in the model leads to quantitative agreement with experimental data and that tuning of the molecular order by addition of isopropanol vapor allows us to control the interaction forces between the nanoparticles.

Rapid Energy Modeling Workflow Demonstration

DTIC Science & Technology

2013-10-31

one of the few deep-water ports on the West Coast. NBVC Port Hueneme is home to the Pacific Seabees, four Naval Mobile Construction Battalions...the base is to provide training, mobilization and deployment operations for Army, Navy, Air Force, and Marines. The base supports a population of... mobile was used on iPads in the field to create rough 3D building mass form models (Figure 6). This approach allowed teams to identify and model only

A Feasibility Study of Life-Extending Controls for Aircraft Turbine Engines Using a Generic Air Force Model (Preprint)

DTIC Science & Technology

2006-12-01

intelligent control algorithm embedded in the FADEC . This paper evaluates the LEC, based on critical components research, to demonstrate how an...control action, engine component life usage, and designing an intelligent control algorithm embedded in the FADEC . This paper evaluates the LEC, based on...simulation code for each simulator. One is typically configured to operate as a Full- Authority Digital Electronic Controller ( FADEC

Enhancing Ear and Hearing Health Access for Children With Technology and Connectivity.

PubMed

Swanepoel, De Wet

2017-10-12

Technology and connectivity advances are demonstrating increasing potential to improve access of service delivery to persons with hearing loss. This article demonstrates use cases from community-based hearing screening and automated diagnosis of ear disease. This brief report reviews recent evidence for school- and home-based hearing testing in underserved communities using smartphone technologies paired with calibrated headphones. Another area of potential impact facilitated by technology and connectivity is the use of feature extraction algorithms to facilitate automated diagnosis of most common ear conditions from video-otoscopic images. Smartphone hearing screening using calibrated headphones demonstrated equivalent sensitivity and specificity for school-based hearing screening. Automating test sequences with a forced-choice response paradigm allowed persons with minimal training to offer screening in underserved communities. The automated image analysis and diagnosis system for ear disease demonstrated an overall accuracy of 80.6%, which is up to par and exceeds accuracy rates previously reported for general practitioners and pediatricians. The emergence of these tools that capitalize on technology and connectivity advances enables affordable and accessible models of service delivery for community-based ear and hearing care.

Electro-optic deflectors deliver advantages over acousto-optical deflectors in a high resolution, ultra-fast force-clamp optical trap.

PubMed

Woody, Michael S; Capitanio, Marco; Ostap, E Michael; Goldman, Yale E

2018-04-30

We characterized experimental artifacts arising from the non-linear response of acousto-optical deflectors (AODs) in an ultra-fast force-clamp optical trap and have shown that using electro-optical deflectors (EODs) instead eliminates these artifacts. We give an example of the effects of these artifacts in our ultra-fast force clamp studies of the interaction of myosin with actin filaments. The experimental setup, based on the concept of Capitanio et al. [Nat. Methods 9, 1013-1019 (2012)] utilizes a bead-actin-bead dumbbell held in two force-clamped optical traps which apply a load to the dumbbell to move it at a constant velocity. When myosin binds to actin, the filament motion stops quickly as the total force from the optical traps is transferred to the actomyosin attachment. We found that in our setup, AODs were unsuitable for beam steering due to non-linear variations in beam intensity and deflection angle as a function of driving frequency, likely caused by low-amplitude standing acoustic waves in the deflectors. These aberrations caused instability in the force feedback loops leading to artifactual jumps in the trap position. We demonstrate that beam steering with EODs improves the performance of our instrument. Combining the superior beam-steering capability of the EODs, force acquisition via back-focal-plane interferometry, and dual high-speed FPGA-based feedback loops, we apply precise and constant loads to study the dynamics of interactions between actin and myosin. The same concept applies to studies of other biomolecular interactions.

On estimating intraventricular hemodynamic forces from endocardial dynamics: A comparative study with 4D flow MRI.

PubMed

Pedrizzetti, Gianni; Arvidsson, Per M; Töger, Johannes; Borgquist, Rasmus; Domenichini, Federico; Arheden, Håkan; Heiberg, Einar

2017-07-26

Intraventricular pressure gradients or hemodynamic forces, which are their global measure integrated over the left ventricular volume, have a fundamental importance in ventricular function. They may help revealing a sub-optimal cardiac function that is not evident in terms of tissue motion, which is naturally heterogeneous and variable, and can influence cardiac adaptation. However, hemodynamic forces are not utilized in clinical cardiology due to the unavailability of simple non-invasive measurement tools. Hemodynamic forces depend on the intraventricular flow; nevertheless, most of them are imputable to the dynamics of the endocardial flow boundary and to the exchange of momentum across the mitral and aortic orifices. In this study, we introduce a simplified model based on first principles of fluid dynamics that allows estimating hemodynamic forces without knowing the velocity field inside the LV. The model is validated with 3D phase-contrast MRI (known as 4D flow MRI) in 15 subjects, (5 healthy and 10 patients) using the endocardial surface reconstructed from the three standard long-axis projections. Results demonstrate that the model provides consistent estimates for the base-apex component (mean correlation coefficient r=0.77 for instantaneous values and r=0.88 for root mean square) and good estimates of the inferolateral-anteroseptal component (r=0.50 and 0.84, respectively). The present method represents a potential integration to the existing ones quantifying endocardial deformation in MRI and echocardiography to add a physics-based estimation of the corresponding hemodynamic forces. These could help the clinician to early detect sub-clinical diseases and differentiate between different cardiac dysfunctional states. Copyright © 2017 Elsevier Ltd. All rights reserved.

An improved DNA force field for ssDNA interactions with gold nanoparticles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiang, Xiankai; Huai, Ping; Fan, Chunhai

The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones “protecting” hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thusmore » the “protection” by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.« less

An improved DNA force field for ssDNA interactions with gold nanoparticles

NASA Astrophysics Data System (ADS)

Jiang, Xiankai; Gao, Jun; Huynh, Tien; Huai, Ping; Fan, Chunhai; Zhou, Ruhong; Song, Bo

2014-06-01

The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones "protecting" hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thus the "protection" by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.

An improved DNA force field for ssDNA interactions with gold nanoparticles.

PubMed

Jiang, Xiankai; Gao, Jun; Huynh, Tien; Huai, Ping; Fan, Chunhai; Zhou, Ruhong; Song, Bo

2014-06-21

The widespread applications of single-stranded DNA (ssDNA) conjugated gold nanoparticles (AuNPs) have spurred an increasing interest in the interactions between ssDNA and AuNPs. Despite extensive studies using the most sophisticated experimental techniques, the detailed molecular mechanisms still remain largely unknown. Large scale molecular dynamics (MD) simulations can thus be used to supplement experiments by providing complementary information about ssDNA-AuNP interactions. However, up to now, all modern force fields for DNA were developed based on the properties of double-stranded DNA (dsDNA) molecules, which have hydrophilic outer backbones "protecting" hydrophobic inner nucleobases from water. Without the double-helix structure of dsDNA and thus the "protection" by the outer backbone, the nucleobases of ssDNA are directly exposed to solvent, and their behavior in water is very different from that of dsDNA, especially at the interface with nanoparticles. In this work, we have improved the force field of ssDNA for use with nanoparticles, such as AuNPs, based on recent experimental results and quantum mechanics calculations. With the new improved force field, we demonstrated that a poly(A) sequence adsorbed on a AuNP surface is much more stable than a poly(T) sequence, which is consistent with recent experimental observations. On the contrary, the current standard force fields, including AMBER03, CHARMM27, and OPLSAA, all gave erroneous results as compared to experiments. The current improved force field is expected to have wide applications in the study of ssDNA with nanomaterials including AuNPs, which might help promote the development of ssDNA-based biosensors and other bionano-devices.

Low-noise humidity controller for imaging water mediated processes in atomic force microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gaponenko, I., E-mail: iaroslav.gaponenko@unige.ch; Gamperle, L.; Herberg, K.

2016-06-15

We demonstrate the construction of a novel low-noise continuous flow humidity controller and its integration with a commercial variable-temperature atomic force microscope fluid cell, allowing precise control of humidity and temperature at the sample during nanoscale measurements. Based on wet and dry gas mixing, the design allows a high mechanical stability to be achieved by means of an ultrasonic atomiser for the generation of water-saturated gas, improving upon previous bubbler-based architectures. Water content in the flow is measured both at the inflow and outflow of the fluid cell, enabling the monitoring of water condensation and icing, and allowing controlled variationmore » of the sample temperature independently of the humidity. To benchmark the performance of the controller, the results of detailed noise studies and time-based imaging of the formation of ice layers on highly oriented pyrolytic graphite are shown.« less

Aircraft measurements of electrified clouds at Kennedy Space Center, part 3

NASA Technical Reports Server (NTRS)

Jones, J. J.; Winn, W. P.; Hunyady, S. J.; Moore, C. B.; Bullock, J. W.; Fleischhacker, P.

1990-01-01

Flights made by the Special Purpose Test Vehicle for Atmospheric Research (SPTVAR) airplane during a second deployment to Florida during the summer of 1989 are discussed. The findings based on the data gathered are presented. The progress made during the second year of the project is discussed. The summer 1989 study was carried out with the support and guidance of Col. John Madura, Commander of Detachment 11, 2nd Weather Squadron, USAF, at Patrick Air Force Base (PAFB) and Cape Canaveral Air Force Station. The project goals were to develop and demonstrate techniques for measuring the electric field aloft and locating regions of charge during flight within and near clouds; to characterize the electric conditions that are presently identified as a threat to space launch vehicles; and to study the correlation between the electric field aloft and that at Kennedy Space Center's ground-based electric field mill array for a variety of electrified clouds.

Joule heating effects on particle immobilization in insulator-based dielectrophoretic devices.

PubMed

Gallo-Villanueva, Roberto C; Sano, Michael B; Lapizco-Encinas, Blanca H; Davalos, Rafael V

2014-02-01

In this work, the temperature effects due to Joule heating obtained by application of a direct current electric potential were investigated for a microchannel with cylindrical insulating posts employed for insulator-based dielectrophoresis. The conductivity of the suspending medium, the local electric field, and the gradient of the squared electric field, which directly affect the magnitude of the dielectrophoretic force exerted on particles, were computationally simulated employing COMSOL Multiphysics. It was observed that a temperature gradient is formed along the microchannel, which redistributes the conductivity of the suspending medium leading to an increase of the dielectrophoretic force toward the inlet of the channel while decreasing toward the outlet. Experimental results are in good agreement with simulations on the particle-trapping zones anticipated. This study demonstrates the importance of considering Joule heating effects when designing insulator-based dielectrophoresis systems. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Carbon nanotube computer.

PubMed

Shulaker, Max M; Hills, Gage; Patil, Nishant; Wei, Hai; Chen, Hong-Yu; Wong, H-S Philip; Mitra, Subhasish

2013-09-26

The miniaturization of electronic devices has been the principal driving force behind the semiconductor industry, and has brought about major improvements in computational power and energy efficiency. Although advances with silicon-based electronics continue to be made, alternative technologies are being explored. Digital circuits based on transistors fabricated from carbon nanotubes (CNTs) have the potential to outperform silicon by improving the energy-delay product, a metric of energy efficiency, by more than an order of magnitude. Hence, CNTs are an exciting complement to existing semiconductor technologies. Owing to substantial fundamental imperfections inherent in CNTs, however, only very basic circuit blocks have been demonstrated. Here we show how these imperfections can be overcome, and demonstrate the first computer built entirely using CNT-based transistors. The CNT computer runs an operating system that is capable of multitasking: as a demonstration, we perform counting and integer-sorting simultaneously. In addition, we implement 20 different instructions from the commercial MIPS instruction set to demonstrate the generality of our CNT computer. This experimental demonstration is the most complex carbon-based electronic system yet realized. It is a considerable advance because CNTs are prominent among a variety of emerging technologies that are being considered for the next generation of highly energy-efficient electronic systems.

Counting Unfolding Events in Stretched Helices with Induced Oscillation by Optical Tweezers

NASA Astrophysics Data System (ADS)

Bacabac, Rommel Gaud; Otadoy, Roland

Correlation measures based on embedded probe fluctuations, single or paired, are now widely used for characterizing the viscoelastic properties of biological samples. However, more robust applications using this technique are still lacking. Considering that the study of living matter routinely demonstrates new and complex phenomena, mathematical and experimental tools for analysis have to catch up in order to arrive at newer insights. Therefore, we derive ways of probing non-equilibrium events in helical biopolymers provided by stretching beyond thermal forces. We generalize, for the first time, calculations for winding turn probabilities to account for unfolding events in single fibrous biopolymers and globular proteins under tensile stretching using twin optical traps. The approach is based on approximating the ensuing probe fluctuations as originating from a damped harmonic oscillator under oscillatory forcing.

A bi-stable nanoelectromechanical non-volatile memory based on van der Waals force

NASA Astrophysics Data System (ADS)

Soon, Bo Woon; Jiaqiang Ng, Eldwin; Qian, You; Singh, Navab; Julius Tsai, Minglin; Lee, Chengkuo

2013-07-01

By using complementary-metal-oxide-semiconductor processes, a silicon based bi-stable nanoelectromechanical non-volatile memory is fabricated and characterized. The main feature of this device is an 80 nm wide and 3 μm high silicon nanofin (SiNF) of a high aspect ratio (1:35). The switching mechanism is realized by electrostatic actuation between two lateral electrodes, i.e., terminals. Bi-stable hysteresis behavior is demonstrated when the SiNF maintains its contact to one of the two terminals by leveraging on van der Waals force even after voltage bias is turned off. The compelling results indicate that this design is promising for realization of high density non-volatile memory application due to its nano-scale footprint and zero on-hold power consumption.

Note: A short-pulse high-intensity molecular beam valve based on a piezoelectric stack actuator

NASA Astrophysics Data System (ADS)

Abeysekera, Chamara; Joalland, Baptiste; Shi, Yuanyuan; Kamasah, Alexander; Oldham, James M.; Suits, Arthur G.

2014-11-01

Solenoid and piezoelectric disk valves, which are widely used to generate molecular beam pulses, still suffer from significant restrictions, such as pulse durations typically >50 μs, low repetition rates, and limited gas flows and operational times. Much of this arises owing to the limited forces these actuators can achieve. To overcome these limitations, we have developed a new pulsed valve based on a high-force piezoelectric stack actuator. We show here that operation with pulse durations as low as 20 μs and repetition rates up to 100 Hz can be easily achieved by operating the valve in conjunction with a commercial fast high-voltage switch. We outline our design and demonstrate its performance with molecular beam characterization via velocity map ion imaging.

Note: a short-pulse high-intensity molecular beam valve based on a piezoelectric stack actuator.

PubMed

Abeysekera, Chamara; Joalland, Baptiste; Shi, Yuanyuan; Kamasah, Alexander; Oldham, James M; Suits, Arthur G

2014-11-01

Solenoid and piezoelectric disk valves, which are widely used to generate molecular beam pulses, still suffer from significant restrictions, such as pulse durations typically >50 μs, low repetition rates, and limited gas flows and operational times. Much of this arises owing to the limited forces these actuators can achieve. To overcome these limitations, we have developed a new pulsed valve based on a high-force piezoelectric stack actuator. We show here that operation with pulse durations as low as 20 μs and repetition rates up to 100 Hz can be easily achieved by operating the valve in conjunction with a commercial fast high-voltage switch. We outline our design and demonstrate its performance with molecular beam characterization via velocity map ion imaging.

A slow-adapting microfluidic-based tactile sensor

NASA Astrophysics Data System (ADS)

Tseng, W.-Y.; Fisher, J. S.; Prieto, J. L.; Rinaldi, K.; Alapati, G.; Lee, A. P.

2009-08-01

We present a microfluidic-based tactile sensor mimicking the human slow-adapting mechanoreceptor such as Merkel's disc. The sensor is composed of a polyimide (PI)/polydimethylsiloxane (PDMS) multilayer structure. The device uses a hemispherical reservoir filled with electrolyte solution in the PDMS layer, a microchannel in the PI layer and a pair of sensing electrodes below the microchannel as the force transducer. The tactile signal is detected as the impedance change resulting predominantly from the resistance variance due to the electrodes coverage by the 1M NaCl solution and is measured across the electrode pair. The sensor response is linear and the working range is shown to be in the range of 0-1.8 N. The characterization results also demonstrate the sensing of various levels of forces and its long-term signal stability.

Optical trapping, pulling, and Raman spectroscopy of airborne absorbing particles based on negative photophoretic force

NASA Astrophysics Data System (ADS)

Chen, Gui-hua; He, Lin; Wu, Mu-ying; Yang, Guang; Li, Y. Q.

2017-08-01

Optical pulling is the attraction of objects back to the light source by the use of optically induced "negative forces". The light-induced photophoretic force is generated by the momentum transfer between the heating particles and surrounding gas molecules and can be several orders of magnitude larger than the radiation force and gravitation force. Here, we demonstrate that micron-sized absorbing particles can be optically pulled and manipulated towards the light source over a long distance in air with a collimated Gaussian laser beam based on a negative photophoretic force. A variety of airborne absorbing particles can be pulled by this optical pipeline to the region where they are optically trapped with another focused laser beam and their chemical compositions are characterized with Raman spectroscopy. We found that micron-sized particles are pulled over a meter-scale distance in air with a pulling speed of 1-10 cm/s in the optical pulling pipeline and its speed can be controlled by changing the laser intensity. When an aerosol particle is optically trapped with a focused Gaussian beam, we measured its rotation motion around the laser propagation direction and measured its Raman spectroscopy for chemical identification by molecular fingerprints. The centripetal acceleration of the trapped particle as high as 20 times the gravitational acceleration was observed. Optical pulling over large distances with lasers in combination with Raman spectroscopy opens up potential applications for the collection and identification of atmospheric particles.

Sex differences in force attenuation: a clinical assessment of single-leg hop performance on a portable force plate

PubMed Central

Harrison, A D; Ford, K R; Myer, G D; Hewett, T E

2014-01-01

Objective Impaired biomechanics and neuromuscular control have been suggested as probable links to female sex bias in the onset of patellofemoral pain syndrome. There are limited objective, clinical measures for assessment of impaired biomechanics and neuromuscular control. The primary objective of this investigation was to examine sex differences in vertical ground reaction force (vGRF) and force loading rate in young athletes performing maximum, repeated vertical single-leg hops (RVSHs). The authors hypothesised that females would demonstrate greater vGRF and force loading rate than males and show interlimb differences in force attenuation. Design Cross-sectional study. Setting Paediatric sports medicine clinic. Participants 109 Healthy high school, soccer and basketball athletes. Assessment of risk factors Participants performed RVSHs for 15 seconds on a portable force plate with a sampling rate of 400 Hz (Accupower; AMTI, Watertown, Massachusetts, USA). Main outcome measurements Raw vGRF was filtered with a generalised cross-validation spline using a 50-Hz cutoff frequency and then normalised to potential energy. Force loading rate was calculated by dividing normalised vGRF by time-to-peak force. Group means were compared using analysis of variance. Results The females demonstrated significantly greater normalised vGRF (p<0.001) and force loading rate (p<0.001) during landing than their male counterparts. Neither sex demonstrated significant interlimb differences in force attenuation (p>0.05). Conclusions The female athletes may have altered force attenuation capability during RVSHs as identified by increased vGRF and force loading rate compared with the male athletes. Portable force plates may be potential tools to identify altered force attenuation in clinical settings. PMID:19858114

Calibrated work function mapping by Kelvin probe force microscopy

NASA Astrophysics Data System (ADS)

Fernández Garrillo, Pablo A.; Grévin, Benjamin; Chevalier, Nicolas; Borowik, Łukasz

2018-04-01

We propose and demonstrate the implementation of an alternative work function tip calibration procedure for Kelvin probe force microscopy under ultrahigh vacuum, using monocrystalline metallic materials with known crystallographic orientation as reference samples, instead of the often used highly oriented pyrolytic graphite calibration sample. The implementation of this protocol allows the acquisition of absolute and reproducible work function values, with an improved uncertainty with respect to unprepared highly oriented pyrolytic graphite-based protocols. The developed protocol allows the local investigation of absolute work function values over nanostructured samples and can be implemented in electronic structures and devices characterization as demonstrated over a nanostructured semiconductor sample presenting Al0.7Ga0.3As and GaAs layers with variable thickness. Additionally, using our protocol we find that the work function of annealed highly oriented pyrolytic graphite is equal to 4.6 ± 0.03 eV.

Hyperspectral imaging from space: Warfighter-1

NASA Astrophysics Data System (ADS)

Cooley, Thomas; Seigel, Gary; Thorsos, Ivan

1999-01-01

The Air Force Research Laboratory Integrated Space Technology Demonstrations (ISTD) Program Office has partnered with Orbital Sciences Corporation (OSC) to complement the commercial satellite's high-resolution panchromatic imaging and Multispectral imaging (MSI) systems with a moderate resolution Hyperspectral imaging (HSI) spectrometer camera. The program is an advanced technology demonstration utilizing a commercially based space capability to provide unique functionality in remote sensing technology. This leveraging of commercial industry to enhance the value of the Warfighter-1 program utilizes the precepts of acquisition reform and is a significant departure from the old-school method of contracting for government managed large demonstration satellites with long development times and technology obsolescence concerns. The HSI system will be able to detect targets from the spectral signature measured by the hyperspectral camera. The Warfighter-1 program will also demonstrate the utility of the spectral information to theater military commanders and intelligence analysts by transmitting HSI data directly to a mobile ground station that receives and processes the data. After a brief history of the project origins, this paper will present the details of the Warfighter-1 system and expected results from exploitation of HSI data as well as the benefits realized by this collaboration between the Air Force and commercial industry.

Mechanobiocatalysis: Modulating Enzymatic Activity with Mechanical Force

DTIC Science & Technology

2015-09-28

displayed by enzymes and other materials. It was demonstrated that the application of forces to enzymes properly outfitted with polymers resulted in...distortions at the active sites of the corresponding enzymes . For example, polymer-protein composites were found to display photophysical properties that...intrinsic activities displayed by enzymes and other materials. It was demonstrated that the application of forces to enzymes properly outfitted with polymers

Assessing postural stability via the correlation patterns of vertical ground reaction force components.

PubMed

Hong, Chih-Yuan; Guo, Lan-Yuen; Song, Rong; Nagurka, Mark L; Sung, Jia-Li; Yen, Chen-Wen

2016-08-02

Many methods have been proposed to assess the stability of human postural balance by using a force plate. While most of these approaches characterize postural stability by extracting features from the trajectory of the center of pressure (COP), this work develops stability measures derived from components of the ground reaction force (GRF). In comparison with previous GRF-based approaches that extract stability features from the GRF resultant force, this study proposes three feature sets derived from the correlation patterns among the vertical GRF (VGRF) components. The first and second feature sets quantitatively assess the strength and changing speed of the correlation patterns, respectively. The third feature set is used to quantify the stabilizing effect of the GRF coordination patterns on the COP. In addition to experimentally demonstrating the reliability of the proposed features, the efficacy of the proposed features has also been tested by using them to classify two age groups (18-24 and 65-73 years) in quiet standing. The experimental results show that the proposed features are considerably more sensitive to aging than one of the most effective conventional COP features and two recently proposed COM features. By extracting information from the correlation patterns of the VGRF components, this study proposes three sets of features to assess human postural stability during quiet standing. As demonstrated by the experimental results, the proposed features are not only robust to inter-trial variability but also more accurate than the tested COP and COM features in classifying the older and younger age groups. An additional advantage of the proposed approach is that it reduces the force sensing requirement from 3D to 1D, substantially reducing the cost of the force plate measurement system.

Effect of Forcing Function on Nonlinear Acoustic Standing Waves

NASA Technical Reports Server (NTRS)

Finkheiner, Joshua R.; Li, Xiao-Fan; Raman, Ganesh; Daniels, Chris; Steinetz, Bruce

2003-01-01

Nonlinear acoustic standing waves of high amplitude have been demonstrated by utilizing the effects of resonator shape to prevent the pressure waves from entering saturation. Experimentally, nonlinear acoustic standing waves have been generated by shaking an entire resonating cavity. While this promotes more efficient energy transfer than a piston-driven resonator, it also introduces complicated structural dynamics into the system. Experiments have shown that these dynamics result in resonator forcing functions comprised of a sum of several Fourier modes. However, previous numerical studies of the acoustics generated within the resonator assumed simple sinusoidal waves as the driving force. Using a previously developed numerical code, this paper demonstrates the effects of using a forcing function constructed with a series of harmonic sinusoidal waves on resonating cavities. From these results, a method will be demonstrated which allows the direct numerical analysis of experimentally generated nonlinear acoustic waves in resonators driven by harmonic forcing functions.

Knee Contact Force Asymmetries in Patients Who Failed Return-to-Sport Readiness Criteria 6 Months After Anterior Cruciate Ligament Reconstruction

PubMed Central

Gardinier, Emily S.; Di Stasi, Stephanie; Manal, Kurt; Buchanan, Thomas S.; Snyder-Mackler, Lynn

2015-01-01

Background After anterior cruciate ligament (ACL) injury, contact forces are decreased in the injured knee when compared with the uninjured knee. The persistence of contact force asymmetries after ACL reconstruction may increase the risk of reinjury and may play an important role in the development of knee osteoarthritis in these patients. Functional performance may also be useful in identifying patients who demonstrate potentially harmful joint contact force asymmetries after ACL reconstruction. Hypothesis Knee joint contact force asymmetries would be present during gait after ACL reconstruction, and performance on a specific set of validated return-to-sport (RTS) readiness criteria would discriminate between those who demonstrated contact force asymmetries and those who did not. Study Design Descriptive laboratory study. Methods A total of 29 patients with ACL ruptures participated in gait analysis and RTS readiness testing 6 months after reconstruction. Muscle and joint contact forces were estimated using an electromyography (EMG)–driven musculoskeletal model of the knee. The magnitude of typical limb asymmetry in uninjured controls was used to define limits of meaningful limb asymmetry in patients after ACL reconstruction. The RTS testing included isometric quadriceps strength testing, 4 unilateral hop tests, and 2 self-report questionnaires. Paired t tests were used to assess limb symmetry for peak medial and tibiofemoral contact forces in all patients, and a mixed-design analysis of variance was used to analyze the effect of passing or failing RTS testing on contact force asymmetry. Results Among all patients, neither statistically significant nor meaningful contact force asymmetries were identified. However, patients who failed RTS testing exhibited meaningful contact force asymmetries, with tibiofemoral contact force being significantly lower for the involved knee. Conversely, patients who passed RTS testing exhibited neither significant nor meaningful contact force asymmetries. Conclusion Joint contact force asymmetries during gait are present in some patients 6 months after ACL reconstruction. Patients who demonstrated poor functional performance on RTS readiness testing exhibited significant and meaningful contact force asymmetries. Clinical Relevance When assessing all patients together, variability in the functional status obscured significant and meaningful differences in contact force asymmetry in patients 6 months after ACL reconstruction. These specific RTS readiness criteria appear to differentiate between those who demonstrate joint contact force symmetry after ACL reconstruction and those who do not. PMID:25318940

An instrumented glove for grasp specification in virtual-reality-based point-and-direct telerobotics.

PubMed

Yun, M H; Cannon, D; Freivalds, A; Thomas, G

1997-10-01

Hand posture and force, which define aspects of the way an object is grasped, are features of robotic manipulation. A means for specifying these grasping "flavors" has been developed that uses an instrumented glove equipped with joint and force sensors. The new grasp specification system will be used at the Pennsylvania State University (Penn State) in a Virtual Reality based Point-and-Direct (VR-PAD) robotics implementation. Here, an operator gives directives to a robot in the same natural way that human may direct another. Phrases such as "put that there" cause the robot to define a grasping strategy and motion strategy to complete the task on its own. In the VR-PAD concept, pointing is done using virtual tools such that an operator can appear to graphically grasp real items in live video. Rather than requiring full duplication of forces and kinesthetic movement throughout a task as is required in manual telemanipulation, hand posture and force are now specified only once. The grasp parameters then become object flavors. The robot maintains the specified force and hand posture flavors for an object throughout the task in handling the real workpiece or item of interest. In the Computer integrated Manufacturing (CIM) Laboratory at Penn State, hand posture and force data were collected for manipulating bricks and other items that require varying amounts of force at multiple pressure points. The feasibility of measuring desired grasp characteristics was demonstrated for a modified Cyberglove impregnated with Force-Sensitive Resistor (FSR) (pressure sensors in the fingertips. A joint/force model relating the parameters of finger articulation and pressure to various lifting tasks was validated for the instrumented "wired" glove. Operators using such a modified glove may ultimately be able to configure robot grasping tasks in environments involving hazardous waste remediation, flexible manufacturing, space operations and other flexible robotics applications. In each case, the VR-PAD approach will finesse the computational and delay problems of real-time multiple-degree-of-freedom force feedback telemanipulation.

On the design of a miniature haptic ring for cutaneous force feedback using shape memory alloy actuators

NASA Astrophysics Data System (ADS)

Hwang, Donghyun; Lee, Jaemin; Kim, Keehoon

2017-10-01

This paper proposes a miniature haptic ring that can display touch/pressure and shearing force to the user’s fingerpad. For practical use and wider application of the device, it is developed with the aim of achieving high wearability and mobility/portability as well as cutaneous force feedback functionality. A main body of the device is designed as a ring-shaped lightweight structure with a simple driving mechanism, and thin shape memory alloy (SMA) wires having high energy density are applied as actuating elements. Also, based on a band-type wireless control unit including a wireless data communication module, the whole device could be realized as a wearable mobile haptic device system. These features enable the device to take diverse advantages on functional performances and to provide users with significant usability. In this work, the proposed miniature haptic ring is systematically designed, and its working performances are experimentally evaluated with a fabricated functional prototype. The experimental results obviously demonstrate that the proposed device exhibits higher force-to-weight ratio than conventional finger-wearable haptic devices for cutaneous force feedback. Also, it is investigated that operational performances of the device are strongly influenced by electro-thermomechanical behaviors of the SMA actuator. In addition to the experiments for performance evaluation, we conduct a preliminary user test to assess practical feasibility and usability based on user’s qualitative feedback.

Simulation study of the structure and phase behavior of ceramide bilayers and the role of lipid head group chemistry

PubMed Central

Guo, Shan; Moore, Timothy C.; Iacovella, Christopher R.; Strickland, L. Anderson; McCabe, Clare

2014-01-01

Ceramides are known to be a key component of the stratum corneum, the outermost protective layer of the skin that controls barrier function. In this work, molecular dynamics simulations are used to examine the behavior of ceramide bilayers, focusing on non-hydroxy sphingosine (NS) and non-hydroxy phytosphingosine (NP) ceramides. Here, we propose a modified version of the CHARMM force field for ceramide simulation, which is directly compared to the more commonly used GROMOS-based force field of Berger (Biophys. J. 1997, 72); while both force fields are shown to closely match experiment from a structural standpoint at the physiological temperature of skin, the modified CHARMM force field is better able to capture the thermotropic phase transitions observed in experiment. The role of ceramide chemistry and its impact on structural ordering is examined by comparing ceramide NS to NP, using the validated CHARMM-based force field. These simulations demonstrate that changing from ceramide NS to NP results in changes to the orientation of the OH groups in the lipid headgroups. The arrangement of OH groups perpendicular to the bilayer normal for ceramide NP, verse parallel for NS, results in the formation of a distinct hydrogen bonding network, that is ultimately responsible for shifting the gel-to-liquid phase transition to higher temperature, in direct agreement with experiment. PMID:24501589

Enhancing army analysis capability for warfighter protection: TRADOC-RDECOM M&S decision support environment collaboration

NASA Astrophysics Data System (ADS)

Athmer, Keith; Gaughan, Chris; McDonnell, Joseph S.; Leach, Robert; Davis, Bert; Truong, Kiet; Borum, Howard; Leslie, Richard; Ma, Lein

2012-05-01

The development of an Integrated Base Defense (IBD) is a significant challenge for the Army with many analytical gaps. The IBD problem space is complex, with evolving requirements and a large stakeholder base. In order to evaluate and analyze IBD decisions, the Training & Doctrine Command (TRADOC) Maneuver Support Center of Excellence (MSCoE) led and continues to lead a series of IBD focused experiments and wargames. Modeling and Simulation (M&S) significantly contributes to this effort. To improve IBD M&S capabilities, a collaborative demonstration with the Research, Development and Engineering Command's (RDECOM's) M&S Decision Support Environment (MSDSE) was held in September 2011. The results of this demonstration provided key input to MSCoE IBD related concepts and technologies. Moreover, it established an initial M&S toolset that will significantly improve force protection in combat zones and Army installations worldwide by providing leaders a capability to conduct analysis of defense and mission rehearsals. The demonstration was executed with a "human in the loop" Battle Captain, who was aided by mission command assets such as Base Expeditionary Targeting and Surveillance Sensors-Combined (BETSS-C). The Common Operating Picture was populated and stimulated using Science & Technology (S&T) M&S, allowing for a realistic representation of physical phenomena without the need for real systems. Novel methods were used for simulation orchestration, and for initializing the simulations and Opposing Force (OPFOR) activities. Ultimately, this demonstration showed that the MSDSE is suitable to support TRADOC IBD analyses and that S&T M&S is ready to be used in a demanding simulation environment. This paper will highlight the event's outcomes and lessons identified.

Contact Forces between Single Metal Oxide Nanoparticles in Gas-Phase Applications and Processes

PubMed Central

2017-01-01

In this work we present a comprehensive experimental study to determine the contact forces between individual metal oxide nanoparticles in the gas-phase using atomic force microscopy. In addition, we determined the amount of physisorbed water for each type of particle surface. By comparing our results with mathematical models of the interaction forces, we could demonstrate that classical continuum models of van der Waals and capillary forces alone cannot sufficiently describe the experimental findings. Rather, the discrete nature of the molecules has to be considered, which leads to ordering at the interface and the occurrence of solvation forces. We demonstrate that inclusion of solvation forces in the model leads to quantitative agreement with experimental data and that tuning of the molecular order by addition of isopropanol vapor allows us to control the interaction forces between the nanoparticles. PMID:28186771

COMBINED LABORATORY/FIELD STUDY ON THE USE OF NITRATE FOR IN SITU BIOREMEDIATION OF A FUEL-CONTAMINATED AQUIFER

EPA Science Inventory

A pilot demonstration project was conducted at Eglin Air Force Base, FL, to compare the extent of bioremediation of a fuel-contaminated aquifer using sprinkler application with and without nitrate addition on two adjacent 30 m x 30 m cells. Target compound groups included both B...

Direct folding simulation of helical proteins using an effective polarizable bond force field.

PubMed

Duan, Lili; Zhu, Tong; Ji, Changge; Zhang, Qinggang; Zhang, John Z H

2017-06-14

We report a direct folding study of seven helical proteins (, Trpcage, , C34, N36, , ) ranging from 17 to 53 amino acids through standard molecular dynamics simulations using a recently developed polarizable force field-Effective Polarizable Bond (EPB) method. The backbone RMSDs, radius of gyrations, native contacts and native helix content are in good agreement with the experimental results. Cluster analysis has also verified that these folded structures with the highest population are in good agreement with their corresponding native structures for these proteins. In addition, the free energy landscape of seven proteins in the two dimensional space comprised of RMSD and radius of gyration proved that these folded structures are indeed of the lowest energy conformations. However, when the corresponding simulations were performed using the standard (nonpolarizable) AMBER force fields, no stable folded structures were observed for these proteins. Comparison of the simulation results based on a polarizable EPB force field and a nonpolarizable AMBER force field clearly demonstrates the importance of polarization in the folding of stable helical structures.

Evaluation method for acoustic trapping performance by tracking motion of trapped microparticle

NASA Astrophysics Data System (ADS)

Lim, Hae Gyun; Ham Kim, Hyung; Yoon, Changhan

2018-05-01

We report a method to evaluate the performances of a single-beam acoustic tweezer using a high-frequency ultrasound transducer. The motion of a microparticle trapped by a 45-MHz single-element transducer was captured and analyzed to deduce the magnitude of trapping force. In the proposed method, the motion of a trapped microparticle was analyzed from a series of microscopy images to compute trapping force; thus, no additional equipment such as microfluidics is required. The method could be used to estimate the effective trapping force in an acoustic tweezer experiment to assess cell membrane deformability by attaching a microbead to the surface of a cell and tracking the motion of the trapped bead, which is similar to a bead-based assay that uses optical tweezers. The results showed that the trapping force increased with increasing acoustic intensity and duty factor, but the force eventually reached a plateau at a higher acoustic intensity. They demonstrated that this method could be used as a simple tool to evaluate the performance and to optimize the operating conditions of acoustic tweezers.

Methods for Force Analysis of Overconstrained Parallel Mechanisms: A Review

NASA Astrophysics Data System (ADS)

Liu, Wen-Lan; Xu, Yun-Dou; Yao, Jian-Tao; Zhao, Yong-Sheng

2017-11-01

The force analysis of overconstrained PMs is relatively complex and difficult, for which the methods have always been a research hotspot. However, few literatures analyze the characteristics and application scopes of the various methods, which is not convenient for researchers and engineers to master and adopt them properly. A review of the methods for force analysis of both passive and active overconstrained PMs is presented. The existing force analysis methods for these two kinds of overconstrained PMs are classified according to their main ideas. Each category is briefly demonstrated and evaluated from such aspects as the calculation amount, the comprehensiveness of considering limbs' deformation, and the existence of explicit expressions of the solutions, which provides an important reference for researchers and engineers to quickly find a suitable method. The similarities and differences between the statically indeterminate problem of passive overconstrained PMs and that of active overconstrained PMs are discussed, and a universal method for these two kinds of overconstrained PMs is pointed out. The existing deficiencies and development directions of the force analysis methods for overconstrained systems are indicated based on the overview.

Force-controlled patch clamp of beating cardiac cells.

PubMed

Ossola, Dario; Amarouch, Mohamed-Yassine; Behr, Pascal; Vörös, János; Abriel, Hugues; Zambelli, Tomaso

2015-03-11

From its invention in the 1970s, the patch clamp technique is the gold standard in electrophysiology research and drug screening because it is the only tool enabling accurate investigation of voltage-gated ion channels, which are responsible for action potentials. Because of its key role in drug screening, innovation efforts are being made to reduce its complexity toward more automated systems. While some of these new approaches are being adopted in pharmaceutical companies, conventional patch-clamp remains unmatched in fundamental research due to its versatility. Here, we merged the patch clamp and atomic force microscope (AFM) techniques, thus equipping the patch-clamp with the sensitive AFM force control. This was possible using the FluidFM, a force-controlled nanopipette based on microchanneled AFM cantilevers. First, the compatibility of the system with patch-clamp electronics and its ability to record the activity of voltage-gated ion channels in whole-cell configuration was demonstrated with sodium (NaV1.5) channels. Second, we showed the feasibility of simultaneous recording of membrane current and force development during contraction of isolated cardiomyocytes. Force feedback allowed for a gentle and stable contact between AFM tip and cell membrane enabling serial patch clamping and injection without apparent cell damage.

Unmanned ground vehicles for integrated force protection

NASA Astrophysics Data System (ADS)

Carroll, Daniel M.; Mikell, Kenneth; Denewiler, Thomas

2004-09-01

The combination of Command and Control (C2) systems with Unmanned Ground Vehicles (UGVs) provides Integrated Force Protection from the Robotic Operation Command Center. Autonomous UGVs are directed as Force Projection units. UGV payloads and fixed sensors provide situational awareness while unattended munitions provide a less-than-lethal response capability. Remote resources serve as automated interfaces to legacy physical devices such as manned response vehicles, barrier gates, fence openings, garage doors, and remote power on/off capability for unmanned systems. The Robotic Operations Command Center executes the Multiple Resource Host Architecture (MRHA) to simultaneously control heterogeneous unmanned systems. The MRHA graphically displays video, map, and status for each resource using wireless digital communications for integrated data, video, and audio. Events are prioritized and the user is prompted with audio alerts and text instructions for alarms and warnings. A control hierarchy of missions and duty rosters support autonomous operations. This paper provides an overview of the key technology enablers for Integrated Force Protection with details on a force-on-force scenario to test and demonstrate concept of operations using Unmanned Ground Vehicles. Special attention is given to development and applications for the Remote Detection Challenge and Response (REDCAR) initiative for Integrated Base Defense.

The desmoplakin–intermediate filament linkage regulates cell mechanics

PubMed Central

Broussard, Joshua A.; Yang, Ruiguo; Huang, Changjin; Nathamgari, S. Shiva P.; Beese, Allison M.; Godsel, Lisa M.; Hegazy, Marihan H.; Lee, Sherry; Zhou, Fan; Sniadecki, Nathan J.; Green, Kathleen J.; Espinosa, Horacio D.

2017-01-01

The translation of mechanical forces into biochemical signals plays a central role in guiding normal physiological processes during tissue development and homeostasis. Interfering with this process contributes to cardiovascular disease, cancer progression, and inherited disorders. The actin-based cytoskeleton and its associated adherens junctions are well-established contributors to mechanosensing and transduction machinery; however, the role of the desmosome–intermediate filament (DSM–IF) network is poorly understood in this context. Because a force balance among different cytoskeletal systems is important to maintain normal tissue function, knowing the relative contributions of these structurally integrated systems to cell mechanics is critical. Here we modulated the interaction between DSMs and IFs using mutant forms of desmoplakin, the protein bridging these structures. Using micropillar arrays and atomic force microscopy, we demonstrate that strengthening the DSM–IF interaction increases cell–substrate and cell–cell forces and cell stiffness both in cell pairs and sheets of cells. In contrast, disrupting the interaction leads to a decrease in these forces. These alterations in cell mechanics are abrogated when the actin cytoskeleton is dismantled. These data suggest that the tissue-specific variability in DSM–IF network composition provides an opportunity to differentially regulate tissue mechanics by balancing and tuning forces among cytoskeletal systems. PMID:28495795

Tuning coercive force by adjusting electric potential in solution processed Co/Pt(111) and the mechanism involved

PubMed Central

Chang, Cheng-Hsun-Tony; Kuo, Wei-Hsu; Chang, Yu-Chieh; Tsay, Jyh-Shen; Yau, Shueh-Lin

2017-01-01

A combination of a solution process and the control of the electric potential for magnetism represents a new approach to operating spintronic devices with a highly controlled efficiency and lower power consumption with reduced production cost. As a paradigmatic example, we investigated Co/Pt(111) in the Bloch-wall regime. The depression in coercive force was detected by applying a negative electric potential in an electrolytic solution. The reversible control of coercive force by varying the electric potential within few hundred millivolts is demonstrated. By changing the electric potential in ferromagnetic layers with smaller thicknesses, the efficiency for controlling the tunable coercive force becomes higher. Assuming that the pinning domains are independent of the applied electric potential, an electric potential tuning-magnetic anisotropy energy model was derived and provided insights into our knowledge of the relation between the electric potential tuning coercive force and the thickness of the ferromagnetic layer. Based on the fact that the coercive force can be tuned by changing the electric potential using a solution process, we developed a novel concept of electric-potential-tuned magnetic recording, resulting in a stable recording media with a high degree of writing ability. PMID:28255160

3D-Structured Stretchable Strain Sensors for Out-of-Plane Force Detection.

PubMed

Liu, Zhiyuan; Qi, Dianpeng; Leow, Wan Ru; Yu, Jiancan; Xiloyannnis, Michele; Cappello, Leonardo; Liu, Yaqing; Zhu, Bowen; Jiang, Ying; Chen, Geng; Masia, Lorenzo; Liedberg, Bo; Chen, Xiaodong

2018-05-17

Stretchable strain sensors, as the soft mechanical interface, provide the key mechanical information of the systems for healthcare monitoring, rehabilitation assistance, soft exoskeletal devices, and soft robotics. Stretchable strain sensors based on 2D flat film have been widely developed to monitor the in-plane force applied within the plane where the sensor is placed. However, to comprehensively obtain the mechanical feedback, the capability to detect the out-of-plane force, caused by the interaction outside of the plane where the senor is located, is needed. Herein, a 3D-structured stretchable strain sensor is reported to monitor the out-of-plane force by employing 3D printing in conjunction with out-of-plane capillary force-assisted self-pinning of carbon nanotubes. The 3D-structured sensor possesses large stretchability, multistrain detection, and strain-direction recognition by one single sensor. It is demonstrated that out-of-plane forces induced by the air/fluid flow are reliably monitored and intricate flow details are clearly recorded. The development opens up for the exploration of next-generation 3D stretchable sensors for electronic skin and soft robotics. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KSC-06pd0556

NASA Image and Video Library

2006-03-10

VANDENBERG AIR FORCE BASE, CALIF. - On the ramp adjacent to the runway at Vandenberg Air Force Base in California, a worker positions the vertical fin within the Orbital Sciences L-1011 aircraft. The fin will then be attached to the Space Technology 5's Pegasus rocket which will be mated to the underside of the carrier aircraft. The ST5, which contains three microsatellites with miniaturized redundant components and technologies, is mated to its launch vehicle, Orbital Sciences' Pegasus XL. Each of the ST5 microsatellites will validate New Millennium Program selected technologies, such as the Cold Gas Micro-Thruster and X-Band Transponder Communication System. After deployment from the Pegasus, the micro-satellites will be positioned in a “string of pearls” constellation that demonstrates the ability to position them to perform simultaneous multi-point measurements of the magnetic field using highly sensitive magnetometers. The data will help scientists understand and map the intensity and direction of the Earth’s magnetic field, its relation to space weather events, and affects on our planet. Launch of ST5 and the Pegasus XL will be from underneath the belly of an L-1011 carrier aircraft from Vandenberg Air Force Base.

Precise control of surface electrostatic forces on polymer brush layers with opposite charges for resistance to protein adsorption.

PubMed

Sakata, Sho; Inoue, Yuuki; Ishihara, Kazuhiko

2016-10-01

Various molecular interaction forces are generated during protein adsorption process on material surfaces. Thus, it is necessary to control them to suppress protein adsorption and the subsequent cell and tissue responses. A series of binary copolymer brush layers were prepared via surface-initiated atom transfer radical polymerization, by mixing the cationic monomer unit and anionic monomer unit randomly in various ratios. Surface characterization revealed that the constructed copolymer brush layers exhibited an uniform super-hydrophilic nature and different surface potentials. The strength of the electrostatic interaction forces operating on these mixed-charge copolymer brush surfaces was evaluated quantitatively using force-versus-distance (f-d) curve measurements by atomic force microscopy (AFM) and probes modified by negatively charged carboxyl groups or positively charged amino groups. The electrostatic interaction forces were determined based on the charge ratios of the copolymer brush layers. Notably, the surface containing equivalent cationic/anionic monomer units hardly interacted with both the charged groups. Furthermore, the protein adsorption force and the protein adsorption mass on these surfaces were examined by AFM f-d curve measurement and surface plasmon resonance measurement, respectively. To clarify the influence of the electrostatic interaction on the protein adsorption behavior on the surface, three kinds of proteins having negative, positive, and relatively neutral net charges under physiological conditions were used in this study. We quantitatively demonstrated that the amount of adsorbed proteins on the surfaces would have a strong correlation with the strength of surface-protein interaction forces, and that the strength of surface-protein interaction forces would be determined from the combination between the properties of the electrostatic interaction forces on the surfaces and the charge properties of the proteins. Especially, the copolymer brush surface composed of equivalent cationic/anionic monomer units exhibited no significant interaction forces, and dramatically suppressed the adsorption of proteins regardless of their charge properties. We conclude that the established methodology could elucidate relationship between the protein adsorption behavior and molecular interaction, especially the electrostatic interaction forces, and demonstrated that the suppression of the electrostatic interactions with the ionic functional groups would be important for the development of new polymeric biomaterials with a high repellency of protein adsorption. Copyright © 2016 Elsevier Ltd. All rights reserved.

Investigation of protein folding by coarse-grained molecular dynamics with the UNRES force field.

PubMed

Maisuradze, Gia G; Senet, Patrick; Czaplewski, Cezary; Liwo, Adam; Scheraga, Harold A

2010-04-08

Coarse-grained molecular dynamics simulations offer a dramatic extension of the time-scale of simulations compared to all-atom approaches. In this article, we describe the use of the physics-based united-residue (UNRES) force field, developed in our laboratory, in protein-structure simulations. We demonstrate that this force field offers about a 4000-times extension of the simulation time scale; this feature arises both from averaging out the fast-moving degrees of freedom and reduction of the cost of energy and force calculations compared to all-atom approaches with explicit solvent. With massively parallel computers, microsecond folding simulation times of proteins containing about 1000 residues can be obtained in days. A straightforward application of canonical UNRES/MD simulations, demonstrated with the example of the N-terminal part of the B-domain of staphylococcal protein A (PDB code: 1BDD, a three-alpha-helix bundle), discerns the folding mechanism and determines kinetic parameters by parallel simulations of several hundred or more trajectories. Use of generalized-ensemble techniques, of which the multiplexed replica exchange method proved to be the most effective, enables us to compute thermodynamics of folding and carry out fully physics-based prediction of protein structure, in which the predicted structure is determined as a mean over the most populated ensemble below the folding-transition temperature. By using principal component analysis of the UNRES folding trajectories of the formin-binding protein WW domain (PDB code: 1E0L; a three-stranded antiparallel beta-sheet) and 1BDD, we identified representative structures along the folding pathways and demonstrated that only a few (low-indexed) principal components can capture the main structural features of a protein-folding trajectory; the potentials of mean force calculated along these essential modes exhibit multiple minima, as opposed to those along the remaining modes that are unimodal. In addition, a comparison between the structures that are representative of the minima in the free-energy profile along the essential collective coordinates of protein folding (computed by principal component analysis) and the free-energy profile projected along the virtual-bond dihedral angles gamma of the backbone revealed the key residues involved in the transitions between the different basins of the folding free-energy profile, in agreement with existing experimental data for 1E0L .

Centripetal Force on an Overhead Projector.

ERIC Educational Resources Information Center

Rheam, Harry

1995-01-01

Describes two simple demonstrations of an object moving in a straight line tangent to the circle if centripetal force is removed. Demonstrations use a pie plate and petri dish with ball bearings to illustrate the phenomena on an overhead projector. (LZ)

Comprehensive development and testing of the ASIST-GBV, a screening tool for responding to gender-based violence among women in humanitarian settings.

PubMed

Wirtz, A L; Glass, N; Pham, K; Perrin, N; Rubenstein, L S; Singh, S; Vu, A

2016-01-01

Conflict affected refugees and internally displaced persons (IDPs) are at increased vulnerability to gender-based violence (GBV). Health, psychosocial, and protection services have been implemented in humanitarian settings, but GBV remains under-reported and available services under-utilized. To improve access to existing GBV services and facilitate reporting, the ASIST-GBV screening tool was developed and tested for use in humanitarian settings. This process was completed in four phases: 1) systematic literature review, 2) qualitative research that included individual interviews and focus groups with GBV survivors and service providers, respectively, 3) pilot testing of the developed screening tool, and 4) 3-month implementation testing of the screening tool. Research was conducted among female refugees, aged ≥15 years in Ethiopia, and female IDPs, aged ≥18 years in Colombia. The systematic review and meta-analysis identified a range of GBV experiences and estimated a 21.4 % prevalence of sexual violence (95 % CI:14.9-28.7) among conflict-affected populations. No existing screening tools for GBV in humanitarian settings were identified. Qualitative research with GBV survivors in Ethiopia and Colombia found multiple forms of GBV experienced by refugees and IDPs that occurred during conflict, in transit, and in displaced settings. Identified forms of violence were combined into seven key items on the screening tool: threats of violence, physical violence, forced sex, sexual exploitation, forced pregnancy, forced abortion, and early or forced marriage. Cognitive testing further refined the tool. Pilot testing in both sites demonstrated preliminary feasibility where 64.8 % of participants in Ethiopia and 44.9 % of participants in Colombia were identified with recent (last 12 months) cases of GBV. Implementation testing of the screening tool, conducted as a routine service in camp/district hospitals, allowed for identification of GBV cases and referrals to services. In this phase, 50.6 % of participants in Ethiopia and 63.4 % in Colombia screened positive for recent experiences of GBV. Psychometric testing demonstrated appropriate internal consistency of the tool (Cronbach's α = 0.77) and item response theory demonstrated appropriate discrimination and difficulty of the tool. The ASIST-GBV screening tool has demonstrated utility and validity for use in confidential identification and referral of refugees and IDPs who experience GBV.

Combat and herbicide exposures in Vietnam among a sample of American Legionnaires

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stellman, S.D.; Stellman, J.M.; Sommer, J.F. Jr.

A cross-sectional survey of 6,810 randomly selected members of The American Legion in six states who served in the US Armed Forces during the Vietnam Era was conducted by a mailed questionnaire. Combat stress was evaluated by a previously validated eight-item scale. Exposure to Agent Orange and other herbicides was estimated via exposure probability index previously developed by the authors which uses an algorithm based on the combined Air Force and Joint Services HERBS files of spray data. Two-fifths of the men had served in Southeast Asia, generally at the same time during which the major proportion of herbicides weremore » used; the remaining subjects served elsewhere. Both combat and Agent Orange exposure exhibited distinct, meaningful distributions among Vietnam veterans with service in Southeast Asia and were also correlated with each other. Our analysis demonstrates conclusively that mere presence in Vietnam cannot be used as a proxy for exposure to Agent Orange. Categorization of Vietnam veterans according to herbicide exposure can be successfully accomplished, based on an existing detailed herbicide application data base. This analysis, together with the consistent dose-related results obtained in this series of papers on health and behavioral effects, demonstrates the utility of questionnaire-derived herbicide and combat exposure measures for epidemiologic study of Vietnam veterans.« less

Dielectrophoresis-Enhanced Plasmonic Sensing with Gold Nanohole Arrays

PubMed Central

2015-01-01

We experimentally demonstrate dielectrophoretic concentration of biological analytes on the surface of a gold nanohole array, which concurrently acts as a nanoplasmonic sensor and gradient force generator. The combination of nanohole-enhanced dielectrophoresis, electroosmosis, and extraordinary optical transmission through the periodic gold nanohole array enables real-time label-free detection of analyte molecules in a 5 μL droplet using concentrations as low as 1 pM within a few minutes, which is more than 1000 times faster than purely diffusion-based binding. The nanohole-based optofluidic platform demonstrated here is straightforward to construct, applicable to both charged and neutral molecules, and performs a novel function that cannot be accomplished using conventional surface plasmon resonance sensors. PMID:24646075

High-speed broadband nanomechanical property quantification and imaging of life science materials using atomic force microscope

NASA Astrophysics Data System (ADS)

Ren, Juan

Nanoscale morphological characterization and mechanical properties quantification of soft and biological materials play an important role in areas ranging from nano-composite material synthesis and characterization, cellular mechanics to drug design. Frontier studies in these areas demand the coordination between nanoscale morphological evolution and mechanical behavior variations through simultaneous measurement of these two aspects of properties. Atomic force microscope (AFM) is very promising in achieving such simultaneous measurements at high-speed and broadband owing to its unique capability in applying force stimuli and then, measuring the response at specific locations in a physiologically friendly environment with pico-newton force and nanometer spatial resolution. Challenges, however, arise as current AFM systems are unable to account for the complex and coupled dynamics of the measurement system and probe-sample interaction during high-speed imaging and broadband measurements. In this dissertation, the creation of a set of dynamics and control tools to probe-based high-speed imaging and rapid broadband nanomechanical spectroscopy of soft and biological materials are presented. Firstly, advanced control-based approaches are presented to improve the imaging performance of AFM imaging both in air and in liquid. An adaptive contact mode (ACM) imaging scheme is proposed to replace the traditional contact mode (CM) imaging by addressing the major concerns in both the speed and the force exerted to the sample. In this work, the image distortion caused by the topography tracking error is accounted for in the topography quantification and the quantified sample topography is utilized in a gradient-based optimization method to adjust the cantilever deflection set-point for each scanline closely around the minimal level needed for maintaining a stable probe-sample contact, and a data-driven iterative feedforward control that utilizes a prediction of the next-line tracking is implemented to enhance the sample topography tracking. An adaptive multi-loop mode (AMLM) imaging approach is proposed to substantially increase the imaging speed of tapping mode (TM) while preserving the advantages of TM over CM by integrating an inner-outer feedback control loop to regulate the TM-deflection on top of the conventional TM-amplitude feedback control to improve the sample topography tracking. Experiments demonstrated that the proposed ACM and AMLM are capable of increasing the imaging speed by at least 20 times for conventional contact and tapping mode imaging, respectively, with no loss of imaging quality and well controlled tip-sample interaction force. In addition, an adaptive mode imaging for in-liquid topography quantification on live cells is presented. The experiment results demonstrated that instead of keeping constant scanning speed, the proposed speed optimization scheme is able to increase the imaging speed on live human prostate cancer cells by at least eight-fold with no loss of imaging quality. Secondly, control based approaches to accurate nanomechanical quantification on soft materials for both broadband and in-liquid force-curve measurements are proposed to address the adverse effects caused by the system coupling dynamics and the cantilever acceleration, which were not compensated for by the conventional AFM measurement approach. The proposed nanomechanical measurement approaches are demonstrated through experiments to measure the viscoelastic properties of different polymer samples in air and live human cells in liquid to study the variation of rate-dependent elastic modulus of cervix cancer cell during the epithelial-mesenchymal transition process.

Surface stress mediated image force and torque on an edge dislocation

NASA Astrophysics Data System (ADS)

Raghavendra, R. M.; Divya, Iyer, Ganesh; Kumar, Arun; Subramaniam, Anandh

2018-07-01

The proximity of interfaces gives prominence to image forces experienced by dislocations. The presence of surface stress alters the traction-free boundary conditions existing on free-surfaces and hence is expected to alter the magnitude of the image force. In the current work, using a combined simulation of surface stress and an edge dislocation in a semi-infinite body, we evaluate the configurational effects on the system. We demonstrate that if the extra half-plane of the edge dislocation is parallel to the surface, the image force (glide) is not altered due to surface stress; however, the dislocation experiences a torque. The surface stress breaks the 'climb image force' symmetry, thus leading to non-equivalence between positive and negative climb. We discover an equilibrium position for the edge dislocation in the positive 'climb geometry', arising due to a competition between the interaction of the dislocation stress fields with the surface stress and the image dislocation. Torque in the climb configuration is not affected by surface stress (remains zero). Surface stress is computed using a recently developed two-scale model based on Shuttleworth's idea and image forces using a finite element model developed earlier. The effect of surface stress on the image force and torque experienced by the dislocation monopole is analysed using illustrative 3D models.

Optimal Length Scale for a Turbulent Dynamo.

PubMed

Sadek, Mira; Alexakis, Alexandros; Fauve, Stephan

2016-02-19

We demonstrate that there is an optimal forcing length scale for low Prandtl number dynamo flows that can significantly reduce the required energy injection rate. The investigation is based on simulations of the induction equation in a periodic box of size 2πL. The flows considered are the laminar and turbulent ABC flows forced at different forcing wave numbers k_{f}, where the turbulent case is simulated using a subgrid turbulence model. At the smallest allowed forcing wave number k_{f}=k_{min}=1/L the laminar critical magnetic Reynolds number Rm_{c}^{lam} is more than an order of magnitude smaller than the turbulent critical magnetic Reynolds number Rm_{c}^{turb} due to the hindering effect of turbulent fluctuations. We show that this hindering effect is almost suppressed when the forcing wave number k_{f} is increased above an optimum wave number k_{f}L≃4 for which Rm_{c}^{turb} is minimum. At this optimal wave number, Rm_{c}^{turb} is smaller by more than a factor of 10 than the case forced in k_{f}=1. This leads to a reduction of the energy injection rate by 3 orders of magnitude when compared to the case where the system is forced at the largest scales and thus provides a new strategy for the design of a fully turbulent experimental dynamo.

Rapid and label-free separation of Burkitt's lymphoma cells from red blood cells by optically-induced electrokinetics.

PubMed

Liang, Wenfeng; Zhao, Yuliang; Liu, Lianqing; Wang, Yuechao; Dong, Zaili; Li, Wen Jung; Lee, Gwo-Bin; Xiao, Xiubin; Zhang, Weijing

2014-01-01

Early stage detection of lymphoma cells is invaluable for providing reliable prognosis to patients. However, the purity of lymphoma cells in extracted samples from human patients' marrow is typically low. To address this issue, we report here our work on using optically-induced dielectrophoresis (ODEP) force to rapidly purify Raji cells' (a type of Burkitt's lymphoma cell) sample from red blood cells (RBCs) with a label-free process. This method utilizes dynamically moving virtual electrodes to induce negative ODEP force of varying magnitudes on the Raji cells and RBCs in an optically-induced electrokinetics (OEK) chip. Polarization models for the two types of cells that reflect their discriminate electrical properties were established. Then, the cells' differential velocities caused by a specific ODEP force field were obtained by a finite element simulation model, thereby established the theoretical basis that the two types of cells could be separated using an ODEP force field. To ensure that the ODEP force dominated the separation process, a comparison of the ODEP force with other significant electrokinetics forces was conducted using numerical results. Furthermore, the performance of the ODEP-based approach for separating Raji cells from RBCs was experimentally investigated. The results showed that these two types of cells, with different concentration ratios, could be separated rapidly using externally-applied electrical field at a driven frequency of 50 kHz at 20 Vpp. In addition, we have found that in order to facilitate ODEP-based cell separation, Raji cells' adhesion to the OEK chip's substrate should be minimized. This paper also presents our experimental results of finding the appropriate bovine serum albumin concentration in an isotonic solution to reduce cell adhesion, while maintaining suitable medium conductivity for electrokinetics-based cell separation. In short, we have demonstrated that OEK technology could be a promising tool for efficient and effective purification of Raji cells from RBCs.

An ultra-fast EOD-based force-clamp detects rapid biomechanical transitions

NASA Astrophysics Data System (ADS)

Woody, Michael S.; Capitanio, Marco; Ostap, E. Michael; Goldman, Yale E.

2017-08-01

We assembled an ultra-fast infrared optical trapping system to detect mechanical events that occur less than a millisecond after a ligand binds to its filamentous substrate, such as myosin undergoing its 5 - 10 nm working stroke after actin binding. The instrument is based on the concept of Capitanio et al.1, in which a polymer bead-actin-bead dumbbell is held in two force-clamped optical traps. A force applied by the traps causes the filament to move at a constant velocity as hydrodynamic drag balances the applied load. When the ligand binds, the filament motion stops within 100 μs as the total force from the optical traps is transferred to the attachment. Subsequent translations signal active motions, such as the magnitude and timing of the motor's working stroke. In our instrument, the beads defining the dumbbell are held in independent force clamps utilizing a field-programmable gate array (FPGA) to update the trap beam positions at 250 kHz. We found that in our setup, acousto-optical deflectors (AODs) steering the beams were unsuitable for this purpose due to a slightly non-linear response in the beam intensity and deflection angle vs. the AOD ultra-sound wavelength, likely caused by low-amplitude standing acoustic waves in the deflectors. These aberrations caused instability in the force feedback loops leading to artefactual 20 nm jumps in position. This type of AOD non-linearity has been reported to be absent in electro-optical deflectors (EODs)2. We demonstrate that replacement of the AODs with EODs improves the performance of our instrument. Combining the superior beam-steering capability of the EODs, force acquisition via back-plane interferometry, and the dual high-speed FPGA-based feedback loops, we smoothly and precisely apply constant loads to study the dynamics of interactions between biological molecules such as actin and myosin.

Implications of movement-related cortical potential for understanding neural adaptations in muscle strength tasks

PubMed Central

2014-01-01

This systematic review aims to provide information about the implications of the movement-related cortical potential (MRCP) in acute and chronic responses to the counter resistance training. The structuring of the methods of this study followed the proposals of the PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses). It was performed an electronically search in Pubmed/Medline and ISI Web of Knowledge data bases, from 1987 to 2013, besides the manual search in the selected references. The following terms were used: Bereitschaftspotential, MRCP, strength and force. The logical operator “AND” was used to combine descriptors and terms used to search publications. At the end, 11 studies attended all the eligibility criteria and the results demonstrated that the behavior of MRCP is altered because of different factors such as: force level, rate of force development, fatigue induced by exercise, and the specific phase of muscular action, leading to an increase in the amplitude in eccentric actions compared to concentric actions, in acute effects. The long-term adaptations demonstrated that the counter resistance training provokes an attenuation in the amplitude in areas related to the movement, which may be caused by neural adaptation occurred in the motor cortex. PMID:24602228

Design and Test Research on Cutting Blade of Corn Harvester Based on Bionic Principle.

PubMed

Tian, Kunpeng; Li, Xianwang; Zhang, Bin; Chen, Qiaomin; Shen, Cheng; Huang, Jicheng

2017-01-01

Existing corn harvester cutting blades have problems associated with large cutting resistance, high energy consumption, and poor cut quality. Using bionics principles, a bionic blade was designed by extracting the cutting tooth profile curve of the B. horsfieldi palate. Using a double-blade cutting device testing system, a single stalk cutting performance contrast test for corn stalks obtained at harvest time was carried out. Results show that bionic blades have superior performance, demonstrated by strong cutting ability and good cut quality. Using statistical analysis of two groups of cutting test data, the average cutting force and cutting energy of bionic blades and ordinary blades were obtained as 480.24 N and 551.31 N and 3.91 J and 4.38 J, respectively. Average maximum cutting force and cutting energy consumption for the bionic blade were reduced by 12.89% and 10.73%, respectively. Variance analysis showed that both blade types had a significant effect on maximum cutting energy and cutting energy required to cut a corn stalk. This demonstrates that bionic blades have better cutting force and energy consumption reduction performance than ordinary blades.

The Effect of Water and Confinement on Self-Assembly of Imidazolium Based Ionic Liquids at Mica Interfaces

PubMed Central

Cheng, H.-W.; Dienemann, J.-N.; Stock, P.; Merola, C.; Chen, Y.-J.; Valtiner, M.

2016-01-01

Tuning chemical structure and molecular layering of ionic liquids (IL) at solid interfaces offers leverage to tailor performance of ILs in applications such as super-capacitors, catalysis or lubrication. Recent experimental interpretations suggest that ILs containing cations with long hydrophobic tails form well-ordered bilayers at interfaces. Here we demonstrate that interfacial bilayer formation is not an intrinsic quality of hydrophobic ILs. In contrast, bilayer formation is triggered by boundary conditions including confinement, surface charging and humidity present in the IL. Therefore, we performed force versus distance profiles using atomic force microscopy and the surface forces apparatus. Our results support models of disperse low-density bilayer formation in confined situations, at high surface charging and/or in the presence of water. Conversely, interfacial structuring of long-chain ILs in dry environments and at low surface charging is disordered and dominated by bulk structuring. Our results demonstrate that boundary conditions such as charging, confinement and doping by impurities have decisive influence on structure formation of ILs at interfaces. As such, these results have important implications for understanding the behavior of solid/IL interfaces as they significantly extend previous interpretations. PMID:27452615

The Effect of Water and Confinement on Self-Assembly of Imidazolium Based Ionic Liquids at Mica Interfaces.

PubMed

Cheng, H-W; Dienemann, J-N; Stock, P; Merola, C; Chen, Y-J; Valtiner, M

2016-07-25

Tuning chemical structure and molecular layering of ionic liquids (IL) at solid interfaces offers leverage to tailor performance of ILs in applications such as super-capacitors, catalysis or lubrication. Recent experimental interpretations suggest that ILs containing cations with long hydrophobic tails form well-ordered bilayers at interfaces. Here we demonstrate that interfacial bilayer formation is not an intrinsic quality of hydrophobic ILs. In contrast, bilayer formation is triggered by boundary conditions including confinement, surface charging and humidity present in the IL. Therefore, we performed force versus distance profiles using atomic force microscopy and the surface forces apparatus. Our results support models of disperse low-density bilayer formation in confined situations, at high surface charging and/or in the presence of water. Conversely, interfacial structuring of long-chain ILs in dry environments and at low surface charging is disordered and dominated by bulk structuring. Our results demonstrate that boundary conditions such as charging, confinement and doping by impurities have decisive influence on structure formation of ILs at interfaces. As such, these results have important implications for understanding the behavior of solid/IL interfaces as they significantly extend previous interpretations.

KSC-2015-1302

NASA Image and Video Library

2015-02-07

CAPE CANAVERAL, Fla. – Col. D. Jason Cothern, Space Demonstrations Division chief at Kirtland Air Force Base in Albuquerque, New Mexico, listens to a question from a member of the news media during a briefing regarding NOAA’s Deep Space Climate Observatory spacecraft, or DSCOVR. DSCOVR will launch aboard a SpaceX Falcon 9 rocket. The mission is a partnership between NOAA, NASA and the U.S. Air Force. DSCOVR will maintain the nation's real-time solar wind monitoring capabilities which are critical to the accuracy and lead time of NOAA's space weather alerts and forecasts. To learn more about DSCOVR, visit http://www.nesdis.noaa.gov/DSCOVR. Photo credit: NASA/Kim Shiflett

Optical pulling of airborne absorbing particles and smut spores over a meter-scale distance with negative photophoretic force

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lin, Jinda; Hart, Adam G.; Li, Yong-qing, E-mail: liy@ecu.edu

2015-04-27

We demonstrate optical pulling of single light-absorbing particles and smut spores in air over a meter-scale distance using a single collimated laser beam based on negative photophoretic force. The micron-sized particles are pulled towards the light source at a constant speed of 1–10 cm/s in the optical pulling pipeline while undergoing transverse rotation at 0.2–10 kHz. The pulled particles can be manipulated and precisely positioned on the entrance window with an accuracy of ∼20 μm, and their chemical compositions can be characterized with micro-Raman spectroscopy.

Does Hooke's law work in helical nanosprings?

PubMed

Ben, Sudong; Zhao, Junhua; Rabczuk, Timon

2015-08-28

Hooke's law is a principle of physics that states that the force needed to extend a spring by some distance is proportional to that distance. The law is always valid for an initial portion of the elastic range for nearly all helical macrosprings. Here we report the sharp nonlinear force-displacement relation of tightly wound helical carbon nanotubes at even small displacement via a molecular mechanics model. We demonstrate that the van der Waals (vdW) interaction between the intertube walls dominates the nonlinear relation based on our analytical expressions. This study provides physical insights into the origin of huge nonlinearity of the helical nanosprings.

Comparison of the lateral retention forces on sessile and pendant water drops on a solid surface

NASA Astrophysics Data System (ADS)

de la Madrid, Rafael; Whitehead, Taylor; Irwin, George M.

2015-06-01

We present a simple experiment that demonstrates how a water drop hanging from a Plexiglas surface (pendant drop) experiences a lateral retention force that is comparable to, and in some cases larger than, the lateral retention force on a drop resting on top of the surface (sessile drop). The experiment also affords a simple demonstration of the Coriolis effect in two dimensions.

FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness.

PubMed

Kong, Hyun Joon; Polte, Thomas R; Alsberg, Eben; Mooney, David J

2005-03-22

The mechanical properties of cell adhesion substrates regulate cell phenotype, but the mechanism of this relation is currently unclear. It may involve the magnitude of traction force applied by the cell, and/or the ability of the cells to rearrange the cell adhesion molecules presented from the material. In this study, we describe a FRET technique that can be used to evaluate the mechanics of cell-material interactions at the molecular level and simultaneously quantify the cell-based nanoscale rearrangement of the material itself. We found that these events depended on the mechanical rigidity of the adhesion substrate. Furthermore, both the proliferation and differentiation of preosteoblasts (MC3T3-E1) correlated to the magnitude of force that cells generate to cluster the cell adhesion ligands, but not the extent of ligand clustering. Together, these data demonstrate the utility of FRET in analyzing cell-material interactions, and suggest that regulation of phenotype with substrate stiffness is related to alterations in cellular traction forces.

Bond rupture between colloidal particles with a depletion interaction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Whitaker, Kathryn A.; Furst, Eric M., E-mail: furst@udel.edu

The force required to break the bonds of a depletion gel is measured by dynamically loading pairs of colloidal particles suspended in a solution of a nonadsorbing polymer. Sterically stabilized poly(methyl methacrylate) colloids that are 2.7 μm diameter are brought into contact in a solvent mixture of cyclohexane-cyclohexyl bromide and polystyrene polymer depletant. The particle pairs are subject to a tensile load at a constant loading rate over many approach-retraction cycles. The stochastic nature of the thermal rupture events results in a distribution of bond rupture forces with an average magnitude and variance that increases with increasing depletant concentration. The measuredmore » force distribution is described by the flux of particle pairs sampling the energy barrier of the bond interaction potential based on the Asakura–Oosawa depletion model. A transition state model demonstrates the significance of lubrication hydrodynamic interactions and the effect of the applied loading rate on the rupture force of bonds in a depletion gel.« less

Interfacial fluid instabilities and Kapitsa pendula.

PubMed

Krieger, Madison S

2017-07-01

The onset and development of instabilities is one of the central problems in fluid mechanics. Here we develop a connection between instabilities of free fluid interfaces and inverted pendula. When acted upon solely by the gravitational force, the inverted pendulum is unstable. This position can be stabilized by the Kapitsa phenomenon, in which high-frequency low-amplitude vertical vibrations of the base creates a fictitious force which opposes the gravitational force. By transforming the dynamical equations governing a fluid interface into an appropriate pendulum-type equation, we demonstrate how stability can be induced in fluid systems by properly tuned vibrations. We construct a "dictionary"-type relationship between various pendula and the classical Rayleigh-Taylor, Kelvin-Helmholtz, Rayleigh-Plateau and the self-gravitational instabilities. This makes several results in control theory and dynamical systems directly applicable to the study of tunable fluid instabilities, where the critical wavelength depends on the external forces or the instability is suppressed entirely. We suggest some applications and instances of the effect ranging in scale from microns to the radius of a galaxy.

In silico screening of drug-membrane thermodynamics reveals linear relations between bulk partitioning and the potential of mean force

NASA Astrophysics Data System (ADS)

Menichetti, Roberto; Kanekal, Kiran H.; Kremer, Kurt; Bereau, Tristan

2017-09-01

The partitioning of small molecules in cell membranes—a key parameter for pharmaceutical applications—typically relies on experimentally available bulk partitioning coefficients. Computer simulations provide a structural resolution of the insertion thermodynamics via the potential of mean force but require significant sampling at the atomistic level. Here, we introduce high-throughput coarse-grained molecular dynamics simulations to screen thermodynamic properties. This application of physics-based models in a large-scale study of small molecules establishes linear relationships between partitioning coefficients and key features of the potential of mean force. This allows us to predict the structure of the insertion from bulk experimental measurements for more than 400 000 compounds. The potential of mean force hereby becomes an easily accessible quantity—already recognized for its high predictability of certain properties, e.g., passive permeation. Further, we demonstrate how coarse graining helps reduce the size of chemical space, enabling a hierarchical approach to screening small molecules.

Pull-in instability of paddle-type and double-sided NEMS sensors under the accelerating force

NASA Astrophysics Data System (ADS)

Keivani, M.; Khorsandi, J.; Mokhtari, J.; Kanani, A.; Abadian, N.; Abadyan, M.

2016-02-01

Paddle-type and double-sided nanostructures are potential for use as accelerometers in flying vehicles and aerospace applications. Herein the pull-in instability of the cantilever paddle-type and double-sided sensors in the Casimir regime are investigated under the acceleration. The D'Alembert principle is employed to transform the accelerating system into an equivalent static system by incorporating the accelerating force. Based on the couple stress theory (CST), the size-dependent constitutive equations of the sensors are derived. The governing nonlinear equations are solved by two approaches, i.e. modified variational iteration method and finite difference method. The influences of the Casimir force, geometrical parameters, acceleration and the size phenomenon on the instability performance have been demonstrated. The obtained results are beneficial to design and fabricate paddle-type and double-sided accelerometers.

The relationship between retirement life cycle changes and older men's labor force participation rates.

PubMed

Hayward, M D; Crimmins, E M; Wray, L A

1994-09-01

This study probes the utility of older men's labor force participation rates (LFPRs) as indicators of the work-to-retirement transition. Specific attention is directed at how shifts in the retirement life cycle are related to LFPRs. Based on Current Population Survey data for the 1970s, a life table modeling approach showed that LFPRs are relatively weak indicators of the work-to-retirement transition. This was demonstrated by the relative stability in older men's age profiles of LFPRs despite significant changes in the timing and "organization" of the work-to-retirement transition. The 1970s evidenced a contraction of the main career and the expansion of both post-retirement work activity and retirement, yet none of these changes substantially altered the age profiles of older men's labor force participation rates.

Thrust performance of unsteady propulsors using a novel measurement system, and corresponding wake patterns

PubMed Central

Clark, Richard P.; Smits, Alexander J.

2009-01-01

An apparatus is described for the measurement of unsteady thrust and propulsive efficiency produced by biologically inspired oscillating hydrodynamic propulsors. Force measurement is achieved using a strain-gauge-based force transducer, augmented with a lever to amplify or attenuate the applied force and control the measurement sensitivity and natural frequency of vibration. The lever can be used to tune the system to a specific application and it is shown that, using the lever, the stiffness can be made to increase more rapidly than the measurement sensitivity decreases. Efficiency is computed from measurements of the time-averaged power imparted to the fluid. The apparatus is applied to two different propulsors, demonstrating the versatility of the system; wake visualizations are examined, which provide insight into the physical mechanisms of efficient propulsion. PMID:19946574

ENSO Modulations due to Interannual Variability of Freshwater Forcing and Ocean Biology-induced Heating in the Tropical Pacific

PubMed Central

Zhang, Rong-Hua; Gao, Chuan; Kang, Xianbiao; Zhi, Hai; Wang, Zhanggui; Feng, Licheng

2015-01-01

Recent studies have identified clear climate feedbacks associated with interannual variations in freshwater forcing (FWF) and ocean biology-induced heating (OBH) in the tropical Pacific. The interrelationships among the related anomaly fields are analyzed using hybrid coupled model (HCM) simulations to illustrate their combined roles in modulating the El Niño-Southern Oscillation (ENSO). The HCM-based supporting experiments are performed to isolate the related feedbacks, with interannually varying FWF and OBH being represented individually or collectively, which allows their effects to be examined in a clear way. It is demonstrated that the interannual freshwater forcing enhances ENSO variability and slightly prolongs the simulated ENSO period, while the interannual OBH reduces ENSO variability and slightly shortens the ENSO period, with their feedback effects tending to counteract each other. PMID:26678931

Stiffness Control of Surgical Continuum Manipulators

PubMed Central

Mahvash, Mohsen; Dupont, Pierre E.

2013-01-01

This paper introduces the first stiffness controller for continuum robots. The control law is based on an accurate approximation of a continuum robot’s coupled kinematic and static force model. To implement a desired tip stiffness, the controller drives the actuators to positions corresponding to a deflected robot configuration that produces the required tip force for the measured tip position. This approach provides several important advantages. First, it enables the use of robot deflection sensing as a means to both sense and control tip forces. Second, it enables stiffness control to be implemented by modification of existing continuum robot position controllers. The proposed controller is demonstrated experimentally in the context of a concentric tube robot. Results show that the stiffness controller achieves the desired stiffness in steady state, provides good dynamic performance, and exhibits stability during contact transitions. PMID:24273466

Legal and Regulatroy Obstacles to Nuclear Fission Technology in Space

NASA Astrophysics Data System (ADS)

Force, Melissa K.

2013-09-01

In forecasting the prospective use of small nuclear reactors for spacecraft and space-based power stations, the U.S. Air Force describes space as "the ultimate high ground," providing access to every part of the globe. But is it? A report titled "Energy Horizons: United States Air Force Energy Science &Technology Vision 2011-2026," focuses on core Air Force missions in space energy generation, operations and propulsion and recognizes that investments into small modular nuclear fission reactors can be leveraged for space-based systems. However, the report mentions, as an aside, that "potential catastrophic outcomes" are an element to be weighed and provides no insight into the monumental political and legal will required to overcome the mere stigma of nuclear energy, even when referring only to the most benign nuclear power generation systems - RTGs. On the heels of that report, a joint Department of Energy and NASA team published positive results from the demonstration of a uranium- powered fission reactor. The experiment was perhaps most notable for exemplifying just how effective the powerful anti-nuclear lobby has been in the United States: It was the first such demonstration of its kind in nearly fifty years. Space visionaries must anticipate a difficult war, consisting of multiple battles that must be waged in order to obtain a license to fly any but the feeblest of nuclear power sources in space. This paper aims to guide the reader through the obstacles to be overcome before nuclear fission technology can be put to use in space.

Modified Amber Force Field Correctly Models the Conformational Preference for Tandem GA pairs in RNA

PubMed Central

2015-01-01

Molecular mechanics with all-atom models was used to understand the conformational preference of tandem guanine-adenine (GA) noncanonical pairs in RNA. These tandem GA pairs play important roles in determining stability, flexibility, and structural dynamics of RNA tertiary structures. Previous solution structures showed that these tandem GA pairs adopt either imino (cis Watson–Crick/Watson–Crick A-G) or sheared (trans Hoogsteen/sugar edge A-G) conformations depending on the sequence and orientation of the adjacent closing base pairs. The solution structures (GCGGACGC)2 [Biochemistry, 1996, 35, 9677–9689] and (GCGGAUGC)2 [Biochemistry, 2007, 46, 1511–1522] demonstrate imino and sheared conformations for the two central GA pairs, respectively. These systems were studied using molecular dynamics and free energy change calculations for conformational changes, using umbrella sampling. For the structures to maintain their native conformations during molecular dynamics simulations, a modification to the standard Amber ff10 force field was required, which allowed the amino group of guanine to leave the plane of the base [J. Chem. Theory Comput., 2009, 5, 2088–2100] and form out-of-plane hydrogen bonds with a cross-strand cytosine or uracil. The requirement for this modification suggests the importance of out-of-plane hydrogen bonds in stabilizing the native structures. Free energy change calculations for each sequence demonstrated the correct conformational preference when the force field modification was used, but the extent of the preference is underestimated. PMID:24803859

Soviet News and Propaganda Analysis Based on RED STAR (The Official Newspaper of the Soviet Defense Establishment) for the Period 1-30 June 1983, Volume 3, Number 6.

DTIC Science & Technology

1983-01-01

Camp. " Israelis use force and terror to stop demonstrations. June 6 is a Day of National Mourning Cn Lebanon). Demonstrations protest Israeli oc...cupation. " Israelis continue arrests, acts of terror , and oppression of the Lebanese people. " Oppression continues - many civilians are arrested in Sidon...is an important comunication technique, because it attracts attention and generally is popular with the audience. During the past two years, the

New LMI based gain-scheduling control for recovering contact-free operation of a magnetically levitated rotor

NASA Astrophysics Data System (ADS)

Wang, M.; Cole, M. O. T.; Keogh, P. S.

2017-11-01

A new approach for the recovery of contact-free levitation of a rotor supported by active magnetic bearings (AMB) is assessed through control strategy design, system modelling and experimental verification. The rotor is considered to make contact with a touchdown bearing (TDB), which may lead to entrapment in a bi-stable nonlinear response. A linear matrix inequality (LMI) based gain-scheduling H∞ control technique is introduced to recover the rotor to a contact-free state. The controller formulation involves a time-varying effective stiffness parameter, which can be evaluated in terms of forces transmitted through the TDB. Rather than measuring these forces directly, an observer is introduced with a model of the base structure to transform base acceleration signals using polytopic coordinates for controller adjustment. Force transmission to the supporting base structure will occur either through an AMB alone without contact, or through the AMB and TDB with contact and this must be accounted for in the observer design. The controller is verified experimentally in terms of (a) non-contact robust stability and vibration suppression performance; (b) control action for contact-free recovery at typical running speeds with various unbalance and TDB misalignment conditions; and (c) coast-down experimental tests. The results demonstrate the effectiveness of the AMB control action whenever it operates within its dynamic load capacity.

Depth-dependent resistance of granular media to vertical penetration.

PubMed

Brzinski, T A; Mayor, P; Durian, D J

2013-10-18

We measure the quasistatic friction force acting on intruders moving downwards into a granular medium. By utilizing different intruder geometries, we demonstrate that the force acts locally normal to the intruder surface. By altering the hydrostatic loading of grain contacts by a sub-fluidizing airflow through the bed, we demonstrate that the relevant frictional contacts are loaded by gravity rather than by the motion of the intruder itself. Lastly, by measuring the final penetration depth versus airspeed and using an earlier result for inertial drag, we demonstrate that the same quasistatic friction force acts during impact. Altogether this force is set by a friction coefficient, hydrostatic pressure, projectile size and shape, and a dimensionless proportionality constant. The latter is the same in nearly all experiments, and is surprisingly greater than one.

33 CFR 334.630 - Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U...

Code of Federal Regulations, 2010 CFR

2010-07-01

... Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base... Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base...″, longitude 82°33′02.44″; and thence to a point on the shore line of MacDill Air Force Base at latitude 27°50...

A parametric symmetry breaking transducer

NASA Astrophysics Data System (ADS)

Eichler, Alexander; Heugel, Toni L.; Leuch, Anina; Degen, Christian L.; Chitra, R.; Zilberberg, Oded

2018-06-01

Force detectors rely on resonators to transduce forces into a readable signal. Usually, these resonators operate in the linear regime and their signal appears amidst a competing background comprising thermal or quantum fluctuations as well as readout noise. Here, we demonstrate a parametric symmetry breaking transduction method that leads to a robust nonlinear force detection in the presence of noise. The force signal is encoded in the frequency at which the system jumps between two phase states which are inherently protected against phase noise. Consequently, the transduction effectively decouples from readout noise channels. For a controlled demonstration of the method, we experiment with a macroscopic doubly clamped string. Our method provides a promising paradigm for high-precision force detection.

Competency-based education and training in internal medicine.

PubMed

Weinberger, Steven E; Pereira, Anne G; Iobst, William F; Mechaber, Alex J; Bronze, Michael S

2010-12-07

Recent efforts to improve medical education include adopting a new framework based on 6 broad competencies defined by the Accreditation Council for Graduate Medical Education. In this article, the Alliance for Academic Internal Medicine Education Redesign Task Force II examines the advantages and challenges of a competency-based educational framework for medical residents. Efforts to refine specific competencies by developing detailed milestones are described, and examples of training program initiatives using a competency-based approach are presented. Meeting the challenges of a competency-based framework and supporting these educational innovations require a robust faculty development program. Challenges to competency-based education include teaching and evaluating the competencies related to practice-based learning and improvement and systems-based practice, as well as implementing a flexible time frame to achieve competencies. However, the Alliance for Academic Internal Medicine Education Redesign Task Force II does not favor reducing internal medicine training to less than 36 months as part of competency-based education. Rather, the 36-month time frame should allow for remediation to address deficiencies in achieving competencies and for diverse enrichment experiences in such areas as quality of care and practice improvement for residents who have demonstrated skills in all required competencies.

A multi-scale residual-based anti-hourglass control for compatible staggered Lagrangian hydrodynamics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kucharik, M.; Scovazzi, Guglielmo; Shashkov, Mikhail Jurievich

Hourglassing is a well-known pathological numerical artifact affecting the robustness and accuracy of Lagrangian methods. There exist a large number of hourglass control/suppression strategies. In the community of the staggered compatible Lagrangian methods, the approach of sub-zonal pressure forces is among the most widely used. However, this approach is known to add numerical strength to the solution, which can cause potential problems in certain types of simulations, for instance in simulations of various instabilities. To avoid this complication, we have adapted the multi-scale residual-based stabilization typically used in the finite element approach for staggered compatible framework. In this study, wemore » describe two discretizations of the new approach and demonstrate their properties and compare with the method of sub-zonal pressure forces on selected numerical problems.« less

STS-72 Mission Specialist Koichi Wakata during ASCAN training

NASA Image and Video Library

1995-01-09

S95-00355 (12-14 September 1992) --- Astronaut candidate Koichi Wakata prepares to jump off a box during a parachute landing demonstration at Vance Air Force Base. This portion of the training is designed to familiarize the trainees with the proper way to hit the ground following a parachute jump. Looking on are astronaut candidates Michael L. Gernhardt (left) and Andrew W. S. Thomas (second left), along with a United States Air Force (USAF) instructor. Wakata, representing Japan's National Space Development Agency (NASDA), is one of seven international mission specialist candidates who joined 19 United States astronaut candidates, including Gernhardt and Thomas, for the three-day parachute/survival training school at the Oklahoma Base. EDITORS NOTE: Since this photograph was taken, Gernhardt, Wakata and Thomas have been named as mission specialists for the STS-69, STS-72 and STS-77 missions, respectively.

Note: A short-pulse high-intensity molecular beam valve based on a piezoelectric stack actuator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abeysekera, Chamara; Joalland, Baptiste; Shi, Yuanyuan

2014-11-15

Solenoid and piezoelectric disk valves, which are widely used to generate molecular beam pulses, still suffer from significant restrictions, such as pulse durations typically >50 μs, low repetition rates, and limited gas flows and operational times. Much of this arises owing to the limited forces these actuators can achieve. To overcome these limitations, we have developed a new pulsed valve based on a high-force piezoelectric stack actuator. We show here that operation with pulse durations as low as 20 μs and repetition rates up to 100 Hz can be easily achieved by operating the valve in conjunction with a commercialmore » fast high-voltage switch. We outline our design and demonstrate its performance with molecular beam characterization via velocity map ion imaging.« less

A multi-scale residual-based anti-hourglass control for compatible staggered Lagrangian hydrodynamics

DOE PAGES

Kucharik, M.; Scovazzi, Guglielmo; Shashkov, Mikhail Jurievich; ...

2017-10-28

Hourglassing is a well-known pathological numerical artifact affecting the robustness and accuracy of Lagrangian methods. There exist a large number of hourglass control/suppression strategies. In the community of the staggered compatible Lagrangian methods, the approach of sub-zonal pressure forces is among the most widely used. However, this approach is known to add numerical strength to the solution, which can cause potential problems in certain types of simulations, for instance in simulations of various instabilities. To avoid this complication, we have adapted the multi-scale residual-based stabilization typically used in the finite element approach for staggered compatible framework. In this study, wemore » describe two discretizations of the new approach and demonstrate their properties and compare with the method of sub-zonal pressure forces on selected numerical problems.« less

Joule heating effects on particle immobilization in insulator-based dielectrophoretic devices

PubMed Central

Gallo-Villanueva, Roberto C.; Sano, Michael B.; Lapizco-Encinas, Blanca H.; Davalos, Rafael V.

2014-01-01

In this work, the temperature effects due to Joule heating obtained by application of a DC electric potential were investigated for a microchannel with cylindrical insulating posts employed for insulator based dielectrophoresis (iDEP). The conductivity of the suspending medium, the local electric field, and the gradient of the squared electric field, which directly affect the magnitude of the dielectrophoretic force exerted on particles, were computationally simulated employing COMSOL Multiphysics. It was observed that a temperature gradient is formed along the microchannel which redistributes the conductivity of the suspending medium leading to an increase of the dielectrophoretic force towards the inlet of the channel while decreasing towards the outlet. Experimental results are in good agreement with simulations on the particle trapping zones anticipated. This study demonstrates the importance of considering Joule heating effects when designing iDEP systems. PMID:24002905

Factors associated with forced sex among women accessing health services in rural Haiti: implications for the prevention of HIV infection and other sexually transmitted diseases.

PubMed

Smith Fawzi, M C; Lambert, W; Singler, J M; Tanagho, Y; Léandre, F; Nevil, P; Bertrand, D; Claude, M S; Bertrand, J; Louissaint, M; Jeannis, L; Mukherjee, J S; Goldie, S; Salazar, J J; Farmer, P E

2005-02-01

The goals of the current study were to: (1) estimate the prevalence of forced sex among women accessing services at a women's health clinic in rural Haiti; and (2) examine factors associated with forced sex in this population. Based on data from a case-control study of risk factors for sexually transmitted diseases (STDs), a cross-sectional analysis to examine factors associated with forced sex was performed. A number of factors related to gender inequality/socioeconomic vulnerability placed women in rural Haiti at higher risk of forced sex. The strongest factors associated with forced sex in multivariate analyses were: age, length of time in a relationship, occupation of the woman's partner, STD-related symptoms, and factors demonstrating economic vulnerability. The findings suggest that prevention efforts must go beyond provision of information and education to the pursuit of broader initiatives at both local and national levels. At the community level, policy-makers should consider advancing economic opportunities for women who are vulnerable to forced sex. Improving access to community-based income-generating activities may begin to address this problem. However, the viability of these local projects depends largely upon Haiti's 'macro-economic' situation. In order to ensure the success of local initiatives, external humanitarian and development assistance to Haiti should be supported. By broadening the definition of "prevention" interventions, we may begin to address the systemic problems that contribute to the occurrence of forced sex and the increasing incidence of HIV infection throughout the world, such as gender inequality and economic vulnerability. Taking into account factors influencing risk at the local level as well as the macro-level will potentially improve our capacity to reduce the risk of forced sex and the spread of STDs, including HIV infection, for millions of women living in poverty worldwide.

Robot Training With Vector Fields Based on Stroke Survivors' Individual Movement Statistics.

PubMed

Wright, Zachary A; Lazzaro, Emily; Thielbar, Kelly O; Patton, James L; Huang, Felix C

2018-02-01

The wide variation in upper extremity motor impairments among stroke survivors necessitates more intelligent methods of customized therapy. However, current strategies for characterizing individual motor impairments are limited by the use of traditional clinical assessments (e.g., Fugl-Meyer) and simple engineering metrics (e.g., goal-directed performance). Our overall approach is to statistically identify the range of volitional movement capabilities, and then apply a robot-applied force vector field intervention that encourages under-expressed movements. We investigated whether explorative training with such customized force fields would improve stroke survivors' (n = 11) movement patterns in comparison to a control group that trained without forces (n = 11). Force and control groups increased Fugl-Meyer UE scores (average of 1.0 and 1.1, respectively), which is not considered clinically meaningful. Interestingly, participants from both groups demonstrated dramatic increases in their range of velocity during exploration following only six days of training (average increase of 166.4% and 153.7% for the Force and Control group, respectively). While both groups showed evidence of improvement, we also found evidence that customized forces affected learning in a systematic way. When customized forces were active, we observed broader distributions of velocity that were not present in the controls. Second, we found that these changes led to specific changes in unassisted motion. In addition, while the shape of movement distributions changed significantly for both groups, detailed analysis of the velocity distributions revealed that customized forces promoted a greater proportion of favorable changes. Taken together, these results provide encouraging evidence that patient-specific force fields based on individuals' movement statistics can be used to create new movement patterns and shape them in a customized manner. To the best of our knowledge, this paper is the first to directly link engineering assessments of stroke survivors' exploration movement behaviors to the design of customized robot therapy.

Mixed models and reduced/selective integration displacement models for nonlinear analysis of curved beams

NASA Technical Reports Server (NTRS)

Noor, A. K.; Peters, J. M.

1981-01-01

Simple mixed models are developed for use in the geometrically nonlinear analysis of deep arches. A total Lagrangian description of the arch deformation is used, the analytical formulation being based on a form of the nonlinear deep arch theory with the effects of transverse shear deformation included. The fundamental unknowns comprise the six internal forces and generalized displacements of the arch, and the element characteristic arrays are obtained by using Hellinger-Reissner mixed variational principle. The polynomial interpolation functions employed in approximating the forces are one degree lower than those used in approximating the displacements, and the forces are discontinuous at the interelement boundaries. Attention is given to the equivalence between the mixed models developed herein and displacement models based on reduced integration of both the transverse shear and extensional energy terms. The advantages of mixed models over equivalent displacement models are summarized. Numerical results are presented to demonstrate the high accuracy and effectiveness of the mixed models developed and to permit a comparison of their performance with that of other mixed models reported in the literature.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gildersleeve, C.W.

An interdisciplinary analysis of the post-Cold War world to determine the optimal strategy to attain the national interests of the United States, and the requisite logistic structure to support that strategy. The optimal solution is found to be a strategy based on multinational defense centered on a permanent force of United Nations garrison port complexes. This multilateral force would be augmented by as small a national defense force as necessary to ensure national security. The theses endeavors to reconnect the cultural and philosophical past of the United States with its immediate future. National interests are identified through examination of Americanmore » Pragmatism and the philosophies of John Locke and Jean-Jacques Rousseau. To determine the current status of common defense, based upon the Foreign Military Sales system, and analysis of current data is accomplished. Future threats to the United States are examined with special emphasis on nuclear terrorism. The ability of Islamic nations in North Africa and the Middle East to produce significant quantities of uranium is demonstrated. The grave political as well as ongoing environmental consequences of this recent capability are discussed in detail.« less

Detection of stiff nanoparticles within cellular structures by contact resonance atomic force microscopy subsurface nanomechanical imaging.

PubMed

Reggente, Melania; Passeri, Daniele; Angeloni, Livia; Scaramuzzo, Francesca Anna; Barteri, Mario; De Angelis, Francesca; Persiconi, Irene; De Stefano, Maria Egle; Rossi, Marco

2017-05-04

Detecting stiff nanoparticles buried in soft biological matrices by atomic force microscopy (AFM) based techniques represents a new frontier in the field of scanning probe microscopies, originally developed as surface characterization methods. Here we report the detection of stiff (magnetic) nanoparticles (NPs) internalized in cells by using contact resonance AFM (CR-AFM) employed as a potentially non-destructive subsurface characterization tool. Magnetite (Fe 3 O 4 ) NPs were internalized in microglial cells from cerebral cortices of mouse embryos of 18 days by phagocytosis. Nanomechanical imaging of cells was performed by detecting the contact resonance frequencies (CRFs) of an AFM cantilever held in contact with the sample. Agglomerates of NPs internalized in cells were visualized on the basis of the local increase in the contact stiffness with respect to the surrounding biological matrix. A second AFM-based technique for nanomechanical imaging, i.e., HarmoniX™, as well as magnetic force microscopy and light microscopy were used to confirm the CR-AFM results. Thus, CR-AFM was demonstrated as a promising technique for subsurface imaging of nanomaterials in biological samples.

Unsteady Aerodynamic Force Sensing from Measured Strain

NASA Technical Reports Server (NTRS)

Pak, Chan-Gi

2016-01-01

A simple approach for computing unsteady aerodynamic forces from simulated measured strain data is proposed in this study. First, the deflection and slope of the structure are computed from the unsteady strain using the two-step approach. Velocities and accelerations of the structure are computed using the autoregressive moving average model, on-line parameter estimator, low-pass filter, and a least-squares curve fitting method together with analytical derivatives with respect to time. Finally, aerodynamic forces over the wing are computed using modal aerodynamic influence coefficient matrices, a rational function approximation, and a time-marching algorithm. A cantilevered rectangular wing built and tested at the NASA Langley Research Center (Hampton, Virginia, USA) in 1959 is used to validate the simple approach. Unsteady aerodynamic forces as well as wing deflections, velocities, accelerations, and strains are computed using the CFL3D computational fluid dynamics (CFD) code and an MSC/NASTRAN code (MSC Software Corporation, Newport Beach, California, USA), and these CFL3D-based results are assumed as measured quantities. Based on the measured strains, wing deflections, velocities, accelerations, and aerodynamic forces are computed using the proposed approach. These computed deflections, velocities, accelerations, and unsteady aerodynamic forces are compared with the CFL3D/NASTRAN-based results. In general, computed aerodynamic forces based on the lifting surface theory in subsonic speeds are in good agreement with the target aerodynamic forces generated using CFL3D code with the Euler equation. Excellent aeroelastic responses are obtained even with unsteady strain data under the signal to noise ratio of -9.8dB. The deflections, velocities, and accelerations at each sensor location are independent of structural and aerodynamic models. Therefore, the distributed strain data together with the current proposed approaches can be used as distributed deflection, velocity, and acceleration sensors. This research demonstrates the feasibility of obtaining induced drag and lift forces through the use of distributed sensor technology with measured strain data. An active induced drag control system thus can be designed using the two computed aerodynamic forces, induced drag and lift, to improve the fuel efficiency of an aircraft. Interpolation elements between structural finite element grids and the CFD grids and centroids are successfully incorporated with the unsteady aeroelastic computation scheme. The most critical technology for the success of the proposed approach is the robust on-line parameter estimator, since the least-squares curve fitting method depends heavily on aeroelastic system frequencies and damping factors.

A Biomechanical Assessment of Hand/Arm Force with Pneumatic Nail Gun Actuation Systems.

PubMed

Lowe, Brian D; Albers, James; Hudock, Stephen D

2014-09-01

A biomechanical model is presented, and combined with measurements of tip press force, to estimate total user hand force associated with two pneumatic nail gun trigger systems. The contact actuation trigger (CAT) can fire a nail when the user holds the trigger depressed first and then "bumps" the nail gun tip against the workpiece. With a full sequential actuation trigger (SAT) the user must press the tip against the workpiece prior to activating the trigger. The SAT is demonstrably safer in reducing traumatic injury risk, but increases the duration (and magnitude) of tip force exertion. Time integrated (cumulative) hand force was calculated for a single user from measurements of the tip contact force with the workpiece and transfer time between nails as inputs to a static model of the nail gun and workpiece in two nailing task orientations. The model shows the hand force dependence upon the orientation of the workpiece in addition to the trigger system. Based on standard time allowances from work measurement systems (i.e. Methods-Time Measurement - 1) it is proposed that efficient application of hand force with the SAT in maintaining tip contact can reduce force exertion attributable to the sequential actuation trigger to 2-8% (horizontal nailing) and 9-20% (vertical nailing) of the total hand/arm force. The present model is useful for considering differences in cumulative hand/arm force exposure between the SAT and CAT systems and may explain the appeal of the CAT trigger in reducing the user's perception of muscular effort.

A Biomechanical Assessment of Hand/Arm Force with Pneumatic Nail Gun Actuation Systems

PubMed Central

Lowe, Brian D.; Albers, James; Hudock, Stephen D.

2015-01-01

A biomechanical model is presented, and combined with measurements of tip press force, to estimate total user hand force associated with two pneumatic nail gun trigger systems. The contact actuation trigger (CAT) can fire a nail when the user holds the trigger depressed first and then “bumps” the nail gun tip against the workpiece. With a full sequential actuation trigger (SAT) the user must press the tip against the workpiece prior to activating the trigger. The SAT is demonstrably safer in reducing traumatic injury risk, but increases the duration (and magnitude) of tip force exertion. Time integrated (cumulative) hand force was calculated for a single user from measurements of the tip contact force with the workpiece and transfer time between nails as inputs to a static model of the nail gun and workpiece in two nailing task orientations. The model shows the hand force dependence upon the orientation of the workpiece in addition to the trigger system. Based on standard time allowances from work measurement systems (i.e. Methods-Time Measurement - 1) it is proposed that efficient application of hand force with the SAT in maintaining tip contact can reduce force exertion attributable to the sequential actuation trigger to 2–8% (horizontal nailing) and 9–20% (vertical nailing) of the total hand/arm force. The present model is useful for considering differences in cumulative hand/arm force exposure between the SAT and CAT systems and may explain the appeal of the CAT trigger in reducing the user’s perception of muscular effort. PMID:26321780

Direct measurements of intermolecular forces by chemical force microscopy

NASA Astrophysics Data System (ADS)

Vezenov, Dmitri Vitalievich

1999-12-01

Detailed description of intermolecular forces is key to understanding a wide range of phenomena from molecular recognition to materials failure. The unique features of atomic force microscopy (AFM) to make point contact force measurements with ultra high sensitivity and to generate spatial maps of surface topography and forces have been extended to include measurements between well-defined organic molecular groups. Chemical modification of AFM probes with self-assembled monolayers (SAMs) was used to make them sensitive to specific molecular interactions. This novel chemical force microscopy (CFM) technique was used to probe forces between different molecular groups in a range of environments (vacuum, organic liquids and aqueous solutions); measure surface energetics on a nanometer scale; determine pK values of the surface acid and base groups; measure forces to stretch and unbind a short synthetic DNA duplex and map the spatial distribution of specific functional groups and their ionization state. Studies of adhesion forces demonstrated the important contribution of hydrogen bonding to interactions between simple organic functionalities. The chemical identity of the tip and substrate surfaces as well as the medium had a dramatic effect on adhesion between model monolayers. A direct correlation between surface free energy and adhesion forces was established. The adhesion between epoxy polymer and model mixed SAMs varied with the amount of hydrogen bonding component in the monolayers. A consistent interpretation of CFM measurements in polar solvents was provided by contact mechanics models and intermolecular force components theory. Forces between tips and surfaces functionalized with SAMs terminating in acid or base groups depended on their ionization state. A novel method of force titration was introduced for highly local characterization of the pK's of surface functional groups. The pH-dependent changes in friction forces were exploited to map spatially the changes in ionization state on SAM surfaces. The phase contrast in tapping mode AFM between chemically distinct monolayer regions and corresponding adhesion forces were found to be directly correlated. Thus, both friction and intermittent contact CFM images could be interpreted in terms of the strength of intermolecular interactions. CFM was also used to probe biomolecular interactions. Separation forces between complementary oligonucleotide strands were significantly larger than the forces measured between noncomplementary strands and were consistent with the unbinding of a single DNA duplex. CFM data provided a direct measure of the forces required to elastically deform, structurally-transform and separate well-defined, synthetic duplexes into single strand oligonucleotides.

Vertically polarizing undulator with the dynamic compensation of magnetic forces for the next generation of light sources

DOE Office of Scientific and Technical Information (OSTI.GOV)

Strelnikov, N.; Budker Institute of Nuclear Physics, Novosibirsk 630090; Trakhtenberg, E.

2014-11-15

A short prototype (847-mm-long) of an Insertion Device (ID) with the dynamic compensation of ID magnetic forces has been designed, built, and tested at the Advanced Photon Source (APS) of the Argonne National Laboratory. The ID magnetic forces were compensated by the set of conical springs placed along the ID strongback. Well-controlled exponential characteristics of conical springs permitted a very close fit to the ID magnetic forces. Several effects related to the imperfections of actual springs, their mounting and tuning, and how these factors affect the prototype performance has been studied. Finally, series of tests to determine the accuracy andmore » reproducibility of the ID magnetic gap settings have been carried out. Based on the magnetic measurements of the ID B{sub eff}, it has been demonstrated that the magnetic gaps within an operating range were controlled accurately and reproducibly within ±1 μm. Successful tests of this ID prototype led to the design of a 3-m long device based on the same concept. The 3-m long prototype is currently under construction. It represents R and D efforts by the APS toward APS Upgrade Project goals as well as the future generation of IDs for the Linac Coherent Light Source (LCLS)« less

Neutral forces acting on intragenomic variability shape the Escherichia coli regulatory network topology.

PubMed

Ruths, Troy; Nakhleh, Luay

2013-05-07

Cis-regulatory networks (CRNs) play a central role in cellular decision making. Like every other biological system, CRNs undergo evolution, which shapes their properties by a combination of adaptive and nonadaptive evolutionary forces. Teasing apart these forces is an important step toward functional analyses of the different components of CRNs, designing regulatory perturbation experiments, and constructing synthetic networks. Although tests of neutrality and selection based on molecular sequence data exist, no such tests are currently available based on CRNs. In this work, we present a unique genotype model of CRNs that is grounded in a genomic context and demonstrate its use in identifying portions of the CRN with properties explainable by neutral evolutionary forces at the system, subsystem, and operon levels. We leverage our model against experimentally derived data from Escherichia coli. The results of this analysis show statistically significant and substantial neutral trends in properties previously identified as adaptive in origin--degree distribution, clustering coefficient, and motifs--within the E. coli CRN. Our model captures the tightly coupled genome-interactome of an organism and enables analyses of how evolutionary events acting at the genome level, such as mutation, and at the population level, such as genetic drift, give rise to neutral patterns that we can quantify in CRNs.

Micromanipulation and microfabrication for optical microrobotics

NASA Astrophysics Data System (ADS)

Palima, Darwin; Bañas, Andrew Rafael; Vizsnyiczai, Gaszton; Kelemen, Lóránd; Aabo, Thomas; Ormos, Pál.; Glückstad, Jesper

2012-10-01

Robotics can use optics feedback in vision-based control of intelligent robotic guidance systems. With light's miniscule momentum, shrinking robots down to the microscale regime creates opportunities for exploiting optical forces and torques in microrobotic actuation and control. Indeed, the literature on optical trapping and micromanipulation attests to the possibilities for optical microrobotics. This work presents an optical microrobotics perspective on the optical microfabrication and micromanipulation work that we performed. We designed different three-dimensional microstructures and fabricated them by two-photon polymerization. These microstructures were then handled using our biophotonics workstation (BWS) for proof-of-principle demonstrations of optical actuation, akin to 6DOF actuation of robotic micromanipulators. Furthermore, we also show an example of dynamic behavior of the trapped microstructure that can be achieved when using static traps in the BWS. This can be generalized, in the future, towards a structural shaping optimization strategy for optimally controlling microstructures to complement approaches based on lightshaping. We also show that light channeled to microfabricated, free-standing waveguides can be used not only to redirect light for targeted delivery of optical energy but can also for targeted delivery of optical force, which can serve to further extend the manipulation arms in optical robotics. Moreover, light deflection with waveguide also creates a recoil force on the waveguide, which can be exploited for controlling the optical force.

Viscoelastic Properties of Confluent MDCK II Cells Obtained from Force Cycle Experiments.

PubMed

Brückner, Bastian Rouven; Nöding, Helen; Janshoff, Andreas

2017-02-28

The local mechanical properties of cells are frequently probed by force indentation experiments carried out with an atomic force microscope. Application of common contact models provides a single parameter, the Young's modulus, to describe the elastic properties of cells. The viscoelastic response of cells, however, is generally measured in separate microrheological experiments that provide complex shear moduli as a function of time or frequency. Here, we present a straightforward way to obtain rheological properties of cells from regular force distance curves collected in typical force indentation measurements. The method allows us to record the stress-strain relationship as well as changes in the weak power law of the viscoelastic moduli. We derive an analytical function based on the elastic-viscoelastic correspondence principle applied to Hertzian contact mechanics to model both indentation and retraction curves. Rheological properties are described by standard viscoelastic models and the paradigmatic weak power law found to interpret the viscoelastic properties of living cells best. We compare our method with atomic force microscopy-based active oscillatory microrheology and show that the method to determine the power law coefficient is robust against drift and largely independent of the indentation depth and indenter geometry. Cells were subject to Cytochalasin D treatment to provoke a drastic change in the power law coefficient and to demonstrate the feasibility of the approach to capture rheological changes extremely fast and precisely. The method is easily adaptable to different indenter geometries and acquires viscoelastic data with high spatiotemporal resolution. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Department of Defense High Power Laser Program Guidance

DTIC Science & Technology

1994-06-06

Air Force Phillips Laboratory . Through FY94, laboratory operational funding, including civilian... Laboratory Effort and Air Materiel Command Ground-Based Laser (GBL) - Space Control USSPACECOM AF Phillips Laboratory Effort Point Defense Demonstration - Anti...ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING I.GANIZATION Phi 1l i ps Laboratory /LID REPORT numP•R 3550 Aberdeen Avenue, S.E. Kirtland AFB, NM

Human-Centeredness Is Not a Universal Feature of Young Children's Reasoning: Culture and Experience Matter when Reasoning about Biological Entities

ERIC Educational Resources Information Center

Medin, Douglas; Waxman, Sandra; Woodring, Jennie; Washinawatok, Karen

2010-01-01

We consider young children's construals of biological phenomena and the forces that shape them, using Carey's (1985) category-based induction task that demonstrated anthropocentric reasoning in young urban children. Follow-up studies (including our own) have questioned the generality of her results, but they have employed quite different…

ESTCP Munitions Response Live Site Demonstrations, Andersen Air Force Base, Guam

DTIC Science & Technology

2016-04-01

April 2016 Mr. Ryan Steigerwalt Weston Solutions , Inc. Distribution Statement A ESTCP MUNITIONS RESPONSE LIVE SITE...Steigerwalt Weston Solutions , Inc. Version 2 REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this...PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER Weston Solutions 1400 Weston Way West Chester, PA 19380

Joint Command Decision Support System

DTIC Science & Technology

2011-06-01

2010 Olympics and Paralympics games , about a hundred agencies and organizations were involved with the safety and security of the games . Accordingly...Joint Task Force Games (JTFG) staff members were augmented with other Command Staff from Canada Command and Canadian Operational Support Command...CANOSCOM) to create an operational HQ. The scenario used for demonstration was based on fictitious Olympic Games (Breton and Guitouni 2008). The scenario

DOD Joint Bases: Implementation Challenges Demonstrate Need to Reevaluate the Program

DTIC Science & Technology

2014-09-01

members on Guam regarding available housing options on the island . According to the officials, this effort will result in reducing the need for...The Honorable Tim Johnson Chairman The Honorable Mark Kirk Ranking Member Subcommittee on Military Construction, Veterans Affairs, and...installation management function · to Commander, U aval Forces, Marianas Islands , Guam. SECRETARY OF DEFENSE JUSTIFICATION All installations employ

Organic ion association in aqueous phase and ab initio-based force fields: The case of carboxylate/ammonium salts

NASA Astrophysics Data System (ADS)

Houriez, Céline; Vallet, Valérie; Réal, Florent; Meot-Ner Mautner, Michael; Masella, Michel

2017-10-01

We performed molecular dynamics simulations of carboxylate/methylated ammonium ion pairs solvated in bulk water and of carboxylate/methylated ammonium salt solutions at ambient conditions using an ab initio-based polarizable force field whose parameters are assigned to reproduce only high end quantum computations, at the Møller-Plesset second-order perturbation theory/complete basis set limit level, regarding single ions and ion pairs as isolated and micro-hydrated in gas phase. Our results agree with the available experimental results regarding carboxylate/ammonium salt solutions. For instance, our force field approach predicts the percentage of acetate associated with ammonium ions in CH3 COO-/CH3 NH3+ solutions at the 0.2-0.8M concentration scale to range from 14% to 35%, in line with the estimates computed from the experimental ion association constant in liquid water. Moreover our simulations predict the number of water molecules released from the ion first hydration shell to the bulk upon ion association to be about 2.0 ± 0.6 molecules for acetate/protonated amine ion pairs, 3.1 ± 1.5 molecules for the HCOO-/NH4+ pair and 3.3 ± 1.2 molecules for the CH3COO-/(CH3)4N+ pair. For protonated amine-based ion pairs, these values are in line with experiment for alkali/halide pairs solvated in bulk water. All these results demonstrate the promising feature of ab initio-based force fields, i.e., their capacity in accurately modeling chemical systems that cannot be readily investigated using available experimental techniques.

Colloidosome like structures: self-assembly of silica microrods

DOE PAGES

Datskos, P.; Polizos, G.; Bhandari, M.; ...

2016-03-07

Self-assembly of one-dimensional structures is attracting a great deal of interest because assembled structures can provide better properties compared to individual building blocks. We demonstrate silica microrod self-assembly by exploiting Pickering emulsion based strategy. Micron-sized silica rods were synthesized employing previously reported methods based on polyvinylpyrrolidone/ pentanol emulsion droplets. Moreover, rods self-assembled to make structures in the range of z10 40 mm. Smooth rods assembled better than segmented rods. Finally, the assembled structures were bonded by weak van der Waals forces.

Graphene fixed-end beam arrays based on mechanical exfoliation

NASA Astrophysics Data System (ADS)

Li, Peng; You, Zheng; Haugstad, Greg; Cui, Tianhong

2011-06-01

A low-cost mechanical exfoliation method is presented to transfer graphite to graphene for free-standing beam arrays. Nickel film or photoresist is used to peel off and transfer patterned single-layer or multilayer graphene onto substrates with macroscopic continuity. Free-standing graphene beam arrays are fabricated on both silicon and polymer substrates. Their mechanical properties are studied by atomic force microscopy. Finally, a graphene based radio frequency switch is demonstrated, with its pull-in voltage and graphene-silicon junction investigated.

Autonomous operations through onboard artificial intelligence

NASA Technical Reports Server (NTRS)

Sherwood, R. L.; Chien, S.; Castano, R.; Rabideau, G.

2002-01-01

The Autonomous Sciencecraft Experiment (ASE) will fly onboard the Air Force TechSat 21 constellation of three spacecraft scheduled for launch in 2006. ASE uses onboard continuous planning, robust task and goal-based execution, model-based mode identification and reconfiguration, and onboard machine learning and pattern recognition to radically increase science return by enabling intelligent downlink selection and autonomous retargeting. Demonstration of these capabilities in a flight environment will open up tremendous new opportunities in planetary science, space physics, and earth science that would be unreachable without this technology.

Effect of input perturbation on the performance and wake dynamics of aquatic propulsion in heaving flexible foils

NASA Astrophysics Data System (ADS)

Lehn, Andrea M.; Thornycroft, Patrick J. M.; Lauder, George V.; Leftwich, Megan C.

2017-02-01

In this paper we consider the effects of adding high-frequency, low-amplitude perturbations to a smooth sinusoidal base input signal for a heaving panel in a closed loop flow tank. Specifically, 0.1 cm amplitude sinusoidal perturbation waves with frequency fp ranging from 0.5 to 13.0 Hz are added to 1 cm heave sinusoids with base frequencies, fb, ranging from 0.5 to 3.0 Hz. Two thin foils with different flexural stiffness are heaved with the combined input signals in addition to both the high-heave and low-heave signals independently. In all cases, the foils are heaved in a recirculating water channel with an incoming velocity of Vx=10 cm/s and a Reynolds number based on the chord length of Re=17 300 . Results demonstrate that perturbations increase the net axial force, in the streamwise direction, in most cases tested (with the exception of some poor performing flexible foil cases). Most significantly, for a base frequency of 1 Hz, perturbations at 9 Hz result in a 780.7% increase in net streamwise force production. Generally, the higher the perturbation frequency, fp the more axial force generated. However, for the stiffer foil, a clear peak in net force exists at fp=9 Hz , regardless of the base frequency. For the stiffer foil, swimming efficiency at a 1 Hz flapping frequency is increased dramatically with the addition of a perturbation, with reduced efficiency increases at higher flapping frequencies. Likewise, for the flexible foil, swimming efficiency gains are greatest at the lower flapping frequencies. Perturbations alter the wake structure by increasing the vorticity magnitude and increasing the vortex shedding frequency; i.e., more, stronger vortices are produced in each flapping cycle.

Exploring the impact of visual and movement based priming on a motor intervention in the acute phase post-stroke in persons with severe hemiparesis of the upper extremity

PubMed Central

Patel, Jigna; Qiu, Qinyin; Yarossi, Mathew; Merians, Alma; Massood, Supriya; Tunik, Eugene; Adamovich, Sergei; Fluet, Gerard

2016-01-01

Purpose Explore the potential benefits of using priming methods prior to an active hand task in the acute phase post-stroke in persons with severe upper extremity hemiparesis. Methods Five individuals were trained using priming techniques including virtual reality (VR) based visual mirror feedback and contralaterally controlled passive movement strategies prior to training with an active pinch force modulation task. Clinical, kinetic, and neurophysiological measurements were taken pre and post the training period. Clinical measures were taken at six months post training. Results The two priming simulations and active training were well tolerated early after stroke. Priming effects were suggested by increased maximal pinch force immediately after visual and movement based priming. Despite having no clinically observable movement distally, the subjects were able to volitionally coordinate isometric force and muscle activity (EMG) in a pinch tracing task. The Root Mean Square Error (RMSE) of force during the pinch trace task gradually decreased over the training period suggesting learning may have occurred. Changes in motor cortical neurophysiology were seen in the unaffected hemisphere using Transcranial Magnetic Stimulation (TMS) mapping. Significant improvements in motor recovery as measured by the Action Research Arm Test (ARAT) and the Upper Extremity Fugl Meyer Assessment (UEFMA) were demonstrated at six months post training by three of the five subjects. Conclusion This study suggests that an early hand-based intervention using visual and movement based priming activities and a scaled motor task allows participation by persons without the motor control required for traditionally presented rehabilitation and testing. PMID:27636200

14 CFR 25.143 - General.

Code of Federal Regulations, 2012 CFR

2012-01-01

... control forces permitted during the testing required by paragraph (a) through (c) of this section: Force... practices must be followed when demonstrating compliance with the control force limitations for short term... with the control force limitations for long term application that are prescribed in paragraph (d) of...

14 CFR 25.143 - General.

Code of Federal Regulations, 2011 CFR

2011-01-01

... control forces permitted during the testing required by paragraph (a) through (c) of this section: Force... practices must be followed when demonstrating compliance with the control force limitations for short term... with the control force limitations for long term application that are prescribed in paragraph (d) of...

14 CFR 25.143 - General.

Code of Federal Regulations, 2010 CFR

2010-01-01

... control forces permitted during the testing required by paragraph (a) through (c) of this section: Force... practices must be followed when demonstrating compliance with the control force limitations for short term... with the control force limitations for long term application that are prescribed in paragraph (d) of...

14 CFR 25.143 - General.

Code of Federal Regulations, 2013 CFR

2013-01-01

... control forces permitted during the testing required by paragraph (a) through (c) of this section: Force... practices must be followed when demonstrating compliance with the control force limitations for short term... with the control force limitations for long term application that are prescribed in paragraph (d) of...

Inseparable phone books demonstration

NASA Astrophysics Data System (ADS)

Balta, Nuri; Çetin, Ali

2017-05-01

This study is aimed at first introducing a well-known discrepant event; inseparable phone books and second, turning it into an experiment for high school or middle school students. This discrepant event could be used especially to indicate how friction force can be effective in producing an unexpected result. Demonstration, discussion, explanation and experiment steps are presented on how to turn a simple discrepant event into an instructional activity. Results showed the relationships between number of pages and force, as well as between amounts of interleave and force. In addition to these, the mathematical equation for the total force between all interleaved pages is derived. As a conclusion, this study demonstrated that not only can phone books be used, but also ordinary books, to investigate this discrepant event. This experiment can be conducted as an example to show the agreement between theoretical and experimental results along with the confounding variables. This discrepant event can be used to create a cognitive conflict in students’ minds about the concepts of ‘force and motion’ and ‘friction force’.

Rapid formation of size-controllable multicellular spheroids via 3D acoustic tweezers.

PubMed

Chen, Kejie; Wu, Mengxi; Guo, Feng; Li, Peng; Chan, Chung Yu; Mao, Zhangming; Li, Sixing; Ren, Liqiang; Zhang, Rui; Huang, Tony Jun

2016-07-05

The multicellular spheroid is an important 3D cell culture model for drug screening, tissue engineering, and fundamental biological research. Although several spheroid formation methods have been reported, the field still lacks high-throughput and simple fabrication methods to accelerate its adoption in drug development industry. Surface acoustic wave (SAW) based cell manipulation methods, which are known to be non-invasive, flexible, and high-throughput, have not been successfully developed for fabricating 3D cell assemblies or spheroids, due to the limited understanding on SAW-based vertical levitation. In this work, we demonstrated the capability of fabricating multicellular spheroids in the 3D acoustic tweezers platform. Our method used drag force from microstreaming to levitate cells in the vertical direction, and used radiation force from Gor'kov potential to aggregate cells in the horizontal plane. After optimizing the device geometry and input power, we demonstrated the rapid and high-throughput nature of our method by continuously fabricating more than 150 size-controllable spheroids and transferring them to Petri dishes every 30 minutes. The spheroids fabricated by our 3D acoustic tweezers can be cultured for a week with good cell viability. We further demonstrated that spheroids fabricated by this method could be used for drug testing. Unlike the 2D monolayer model, HepG2 spheroids fabricated by the 3D acoustic tweezers manifested distinct drug resistance, which matched existing reports. The 3D acoustic tweezers based method can serve as a novel bio-manufacturing tool to fabricate complex 3D cell assembles for biological research, tissue engineering, and drug development.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goltz, M.N.; Carrothers, T.J.; Hopkins, G.D.

A full-scale study of in situ bioremediation is being planned for implementation at a trichloroethylene (TCE) contaminated site at Edwards Air Force Base. To support the demonstration, laboratory studies of sorption and biodegradation were performed. Column studies conducted with TCE and Edwards aquifer material determined the sorption distribution coefficient to be 0.1 millileters per gram, corresponding to a 1.5 retardation factor. Edwards aquifer material from four different depths was used to study cometabolic TCE biodegradation in the presence of toluene under aerobic conditions. The studies showed steady state TCE removal of greater than or equal to 85% in the microcosms.more » A base line simulation was performed using a computer model which incorporated all significant flow and transport processes. The simulation shows how TCE mass declines in both the dissolved and sorbed phases over the four months planned for the demonstration. Based on these preliminary studies, it appears that the planned operation of the bioremediation system can be expected to result in observable decreases in TCE mass and concentration at the Edwards demonstration site.« less

Ab initio protein structure assembly using continuous structure fragments and optimized knowledge-based force field.

PubMed

Xu, Dong; Zhang, Yang

2012-07-01

Ab initio protein folding is one of the major unsolved problems in computational biology owing to the difficulties in force field design and conformational search. We developed a novel program, QUARK, for template-free protein structure prediction. Query sequences are first broken into fragments of 1-20 residues where multiple fragment structures are retrieved at each position from unrelated experimental structures. Full-length structure models are then assembled from fragments using replica-exchange Monte Carlo simulations, which are guided by a composite knowledge-based force field. A number of novel energy terms and Monte Carlo movements are introduced and the particular contributions to enhancing the efficiency of both force field and search engine are analyzed in detail. QUARK prediction procedure is depicted and tested on the structure modeling of 145 nonhomologous proteins. Although no global templates are used and all fragments from experimental structures with template modeling score >0.5 are excluded, QUARK can successfully construct 3D models of correct folds in one-third cases of short proteins up to 100 residues. In the ninth community-wide Critical Assessment of protein Structure Prediction experiment, QUARK server outperformed the second and third best servers by 18 and 47% based on the cumulative Z-score of global distance test-total scores in the FM category. Although ab initio protein folding remains a significant challenge, these data demonstrate new progress toward the solution of the most important problem in the field. Copyright © 2012 Wiley Periodicals, Inc.

A mechanical mechanism for translocation of ring-shaped helicases on DNA and its demonstration in a macroscopic simulation system

NASA Astrophysics Data System (ADS)

Chou, Y. C.

2018-04-01

The asymmetry in the two-layered ring structure of helicases and the random thermal fluctuations of the helicase and DNA molecules are considered as the bases for the generation of the force required for translocation of the ring-shaped helicase on DNA. The helicase comprises a channel at its center with two unequal ends, through which strands of DNA can pass. The random collisions between the portion of the DNA strand in the central channel and the wall of the channel generate an impulsive force toward the small end. This impulsive force is the starting point for the helicase to translocate along the DNA with the small end in front. Such a physical mechanism may serve as a complementary for the chemomechanical mechanism of the translocation of helicase on DNA. When the helicase arrives at the junction of ssDNA and dsDNA (a fork), the collision between the helicase and the closest base pair may produce a sufficient impulsive force to break the weak hydrogen bond of the base pair. Thus, the helicase may advance and repeat the process of unwinding the dsDNA strand. This mechanism was tested in a macroscopic simulation system where the helicase was simulated using a truncated-cone structure and DNA was simulated with bead chains. Many features of translocation and unwinding such as translocation on ssDNA and dsDNA, unwinding of dsDNA, rewinding, strand switching, and Holliday junction resolution were reproduced.

Best Practices in User Needs/Requirements Generation

DTIC Science & Technology

2000-06-15

to the US Air Force, the framework was tested . All of the organizations demonstrated the existence of the four fundamental activities contained in...SP Air Force Deputy Chief of Staff for Security Police AF/TE Air Force Deputy Chief of Staff for Test and Evaluations AF/XO Air Force Deputy Chief of...Operational Test and Evaluation Center AFPD Air Force Policy Directive AFROC Air Force Requirements Oversight Council AFSOC Air Force Special Operations

33 CFR 334.630 - Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base... Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base...

33 CFR 334.630 - Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base... Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base...

33 CFR 334.630 - Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base... Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base...

33 CFR 334.630 - Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Tampa Bay south of MacDill Air Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base... Force Base, Fla.; small-arms firing range and aircraft jettison, U.S. Air Force, MacDill Air Force Base...

Predicting the behavior of novel sugar carriers for dry powder inhaler formulations via the use of a cohesive-adhesive force balance approach.

PubMed

Hooton, Jennifer C; Jones, Matthew D; Price, Robert

2006-06-01

The aim of this work was to utilize the recently developed cohesive-adhesive balance (CAB) technique for analyzing quantitative AFM measurements to compare the relative forces of interaction of micronized salbutamol sulfate particles and a selection of specifically grown sugar substrates (beta cyclodextrin, lactose, raffinose, trehalose and xylitol). The interfacial behavior was subsequently related to the in-vitro delivery performance of these sugars as carrier particles in dry powder inhalation (DPI) formulations. The CAB analysis indicated that the rank order of adhesion between salbutamol sulfate and the sugars was beta cyclodextrin < lactose < trehalose < raffinose < xylitol. The beta cyclodextrin was the only substrate with which salbutamol sulfate demonstrated a greater cohesive behavior. All other sugars exhibited an adhesive dominance. In-vitro deposition performance of the salbutamol sulfate based carrier DPI formulations showed that the rank order of the fine particle fraction (FPF) was beta cyclodextrin > lactose > raffinose > trehalose > xylitol. A linear correlation (R(2) = 0.9572) was observed between the FPF and cohesive-adhesive ratios of the AFM force measurements. The observed link between CAB analysis of the interactive forces and in-vitro performance of carrier based formulations suggested a fundamental understanding of the relative balance of the various forces of interaction within a dry powder formulation may provide a critical insight into the behavior of these formulations. (c) 2006 Wiley-Liss, Inc. and the American Pharmacists Association

Model-based optical coherence elastography using acoustic radiation force

NASA Astrophysics Data System (ADS)

Aglyamov, Salavat; Wang, Shang; Karpiouk, Andrei; Li, Jiasong; Emelianov, Stanislav; Larin, Kirill V.

2014-02-01

Acoustic Radiation Force (ARF) stimulation is actively used in ultrasound elastography to estimate mechanical properties of tissue. Compared with ultrasound imaging, OCT provides advantage in both spatial resolution and signal-to-noise ratio. Therefore, a combination of ARF and OCT technologies can provide a unique opportunity to measure viscoelastic properties of tissue, especially when the use of high intensity radiation pressure is limited for safety reasons. In this presentation we discuss a newly developed theoretical model of the deformation of a layered viscoelastic medium in response to an acoustic radiation force of short duration. An acoustic impulse was considered as an axisymmetric force generated on the upper surface of the medium. An analytical solution of this problem was obtained using the Hankel transform in frequency domain. It was demonstrated that layers at different depths introduce different frequency responses. To verify the developed model, experiments were performed using tissue-simulating, inhomogeneous phantoms of varying mechanical properties. The Young's modulus of the phantoms was varied from 5 to 50 kPa. A single-element focused ultrasound transducer (3.5 MHz) was used to apply the radiation force with various durations on the surface of phantoms. Displacements on the phantom surface were measured using a phase-sensitive OCT at 25 kHz repetition frequency. The experimental results were in good agreement with the modeling results. Therefore, the proposed theoretical model can be used to reconstruct the mechanical properties of tissue based on ARF/OCT measurements.

Bacterial adhesion force quantification by fluidic force microscopy

NASA Astrophysics Data System (ADS)

Potthoff, Eva; Ossola, Dario; Zambelli, Tomaso; Vorholt, Julia A.

2015-02-01

Quantification of detachment forces between bacteria and substrates facilitates the understanding of the bacterial adhesion process that affects cell physiology and survival. Here, we present a method that allows for serial, single bacterial cell force spectroscopy by combining the force control of atomic force microscopy with microfluidics. Reversible bacterial cell immobilization under physiological conditions on the pyramidal tip of a microchanneled cantilever is achieved by underpressure. Using the fluidic force microscopy technology (FluidFM), we achieve immobilization forces greater than those of state-of-the-art cell-cantilever binding as demonstrated by the detachment of Escherichia coli from polydopamine with recorded forces between 4 and 8 nN for many cells. The contact time and setpoint dependence of the adhesion forces of E. coli and Streptococcus pyogenes, as well as the sequential detachment of bacteria out of a chain, are shown, revealing distinct force patterns in the detachment curves. This study demonstrates the potential of the FluidFM technology for quantitative bacterial adhesion measurements of cell-substrate and cell-cell interactions that are relevant in biofilms and infection biology.Quantification of detachment forces between bacteria and substrates facilitates the understanding of the bacterial adhesion process that affects cell physiology and survival. Here, we present a method that allows for serial, single bacterial cell force spectroscopy by combining the force control of atomic force microscopy with microfluidics. Reversible bacterial cell immobilization under physiological conditions on the pyramidal tip of a microchanneled cantilever is achieved by underpressure. Using the fluidic force microscopy technology (FluidFM), we achieve immobilization forces greater than those of state-of-the-art cell-cantilever binding as demonstrated by the detachment of Escherichia coli from polydopamine with recorded forces between 4 and 8 nN for many cells. The contact time and setpoint dependence of the adhesion forces of E. coli and Streptococcus pyogenes, as well as the sequential detachment of bacteria out of a chain, are shown, revealing distinct force patterns in the detachment curves. This study demonstrates the potential of the FluidFM technology for quantitative bacterial adhesion measurements of cell-substrate and cell-cell interactions that are relevant in biofilms and infection biology. Electronic supplementary information (ESI) available: Video S1. Detachment of a S. pyogenes cell chain from glass substrate. The cantilever is approached on the outermost adherent cell of a chain and four bacteria were then sequentially detached. The sequential cell detachment suddenly stopped after four bacteria. This possibly occurred because bacteria-glass interactions became too strong or the maximal probe retraction was reached. The cells spontaneously detached from the cantilever flipping back on the surface. Fig. S1. (A) Adhesion force-distance and (B) adhesion force-detaching work correlation of E.coli on PLL for setpoints of 1 and 10 nN. Circle: 1 nN setpoint, square: 10 nN. See DOI: 10.1039/c4nr06495j

Silicon-on-insulator multimode-interference waveguide-based arrayed optical tweezers (SMART) for two-dimensional microparticle trapping and manipulation.

PubMed

Lei, Ting; Poon, Andrew W

2013-01-28

We demonstrate two-dimensional optical trapping and manipulation of 1 μm and 2.2 μm polystyrene particles in an 18 μm-thick fluidic cell at a wavelength of 1565 nm using the recently proposed Silicon-on-insulator Multimode-interference (MMI) waveguide-based ARrayed optical Tweezers (SMART) technique. The key component is a 100 μm square-core silicon waveguide with mm length. By tuning the fiber-coupling position at the MMI waveguide input facet, we demonstrate various patterns of arrayed optical tweezers that enable optical trapping and manipulation of particles. We numerically simulate the physical mechanisms involved in the arrayed trap, including the optical force, the heat transfer and the thermal-induced microfluidic flow.

The Impact of AMSR-E Soil Moisture Assimilation on Evapotranspiration Estimation

NASA Technical Reports Server (NTRS)

Peters-Lidard, Christa D.; Kumar, Sujay; Mocko, David; Tian, Yudong

2012-01-01

An assessment ofETestimates for current LDAS systems is provided along with current research that demonstrates improvement in LSM ET estimates due to assimilating satellite-based soil moisture products. Using the Ensemble Kalman Filter in the Land Information System, we assimilate both NASA and Land Parameter Retrieval Model (LPRM) soil moisture products into the Noah LSM Version 3.2 with the North American LDAS phase 2 CNLDAS-2) forcing to mimic the NLDAS-2 configuration. Through comparisons with two global reference ET products, one based on interpolated flux tower data and one from a new satellite ET algorithm, over the NLDAS2 domain, we demonstrate improvement in ET estimates only when assimilating the LPRM soil moisture product.

Optimized coordination of brakes and active steering for a 4WS passenger car.

PubMed

Tavasoli, Ali; Naraghi, Mahyar; Shakeri, Heman

2012-09-01

Optimum coordination of individual brakes and front/rear steering subsystems is presented. The integrated control strategy consists of three modules. A coordinated high-level control determines the body forces/moment required to achieve vehicle motion objectives. The body forces/moment are allocated to braking and steering subsystems through an intermediate unit, which integrates available subsystems based on phase plane notion in an optimal manner. To this end, an optimization problem including several equality and inequality constraints is defined and solved analytically, such that a real-time implementation can be realized without the use of numeric optimization software. A low-level slip-ratio controller works to generate the desired longitudinal forces at small longitudinal slip-ratios, while averting wheel locking at large slip-ratios. The efficiency of the suggested approach is demonstrated through computer simulations. Copyright © 2012 ISA. Published by Elsevier Ltd. All rights reserved.

Active and Progressive Exoskeleton Rehabilitation Using Multisource Information Fusion From EMG and Force-Position EPP.

PubMed

Fan, Yuanjie; Yin, Yuehong

2013-12-01

Although exoskeletons have received enormous attention and have been widely used in gait training and walking assistance in recent years, few reports addressed their application during early poststroke rehabilitation. This paper presents a healthcare technology for active and progressive early rehabilitation using multisource information fusion from surface electromyography and force-position extended physiological proprioception. The active-compliance control based on interaction force between patient and exoskeleton is applied to accelerate the recovery of the neuromuscular function, whereby progressive treatment through timely evaluation contributes to an effective and appropriate physical rehabilitation. Moreover, a clinic-oriented rehabilitation system, wherein a lower extremity exoskeleton with active compliance is mounted on a standing bed, is designed to ensure comfortable and secure rehabilitation according to the structure and control requirements. Preliminary experiments and clinical trial demonstrate valuable information on the feasibility, safety, and effectiveness of the progressive exoskeleton-assisted training.

Nanoscale chemical imaging by photoinduced force microscopy

PubMed Central

Nowak, Derek; Morrison, William; Wickramasinghe, H. Kumar; Jahng, Junghoon; Potma, Eric; Wan, Lei; Ruiz, Ricardo; Albrecht, Thomas R.; Schmidt, Kristin; Frommer, Jane; Sanders, Daniel P.; Park, Sung

2016-01-01

Correlating spatial chemical information with the morphology of closely packed nanostructures remains a challenge for the scientific community. For example, supramolecular self-assembly, which provides a powerful and low-cost way to create nanoscale patterns and engineered nanostructures, is not easily interrogated in real space via existing nondestructive techniques based on optics or electrons. A novel scanning probe technique called infrared photoinduced force microscopy (IR PiFM) directly measures the photoinduced polarizability of the sample in the near field by detecting the time-integrated force between the tip and the sample. By imaging at multiple IR wavelengths corresponding to absorption peaks of different chemical species, PiFM has demonstrated the ability to spatially map nm-scale patterns of the individual chemical components of two different types of self-assembled block copolymer films. With chemical-specific nanometer-scale imaging, PiFM provides a powerful new analytical method for deepening our understanding of nanomaterials. PMID:27051870

Magnetohydrodynamic actuation of droplets for millimetric planar fluidic systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ahmadi, A., E-mail: ali.ahmadi@ubc.ca; McDermid, C. M.; Markley, L.

2016-01-04

In this work, a magnetohydrodynamic method is proposed for the actuation of droplets in small-scale planar fluidic systems, providing an alternative to commonly used methods such as electrowetting-on-dielectric. Elementary droplet-based operations, including transport, merging, and mixing, are demonstrated. The forces acting on millimetric droplets are carefully investigated, with a primary focus on the magnetic actuation force and on the unbalanced capillary forces that arise due to hysteresis. A super-hydrophobic channel is 3D printed to guide the droplets, with thin wires installed as contact electrodes and permanent magnets providing a static magnetic field. It is shown that droplet motion is enhancedmore » by increasing the droplet size and minimizing the electrode contact surface. The effects of channel geometry on threshold voltage and minimum moveable droplet volume are characterized. Finally, the presence of electrolysis is investigated and mitigating strategies are discussed.« less

Magnetic resonance force microscopy of paramagnetic electron spins at millikelvin temperatures.

PubMed

Vinante, A; Wijts, G; Usenko, O; Schinkelshoek, L; Oosterkamp, T H

2011-12-06

Magnetic resonance force microscopy (MRFM) is a powerful technique to detect a small number of spins that relies on force detection by an ultrasoft magnetically tipped cantilever and selective magnetic resonance manipulation of the spins. MRFM would greatly benefit from ultralow temperature operation, because of lower thermomechanical noise and increased thermal spin polarization. Here we demonstrate MRFM operation at temperatures as low as 30 mK, thanks to a recently developed superconducting quantum interference device (SQUID)-based cantilever detection technique, which avoids cantilever overheating. In our experiment, we detect dangling bond paramagnetic centres on a silicon surface down to millikelvin temperatures. Fluctuations of such defects are supposedly linked to 1/f magnetic noise and decoherence in SQUIDs, as well as in several superconducting and single spin qubits. We find evidence that spin diffusion has a key role in the low-temperature spin dynamics.

Indirect boundary force measurements in beam-like structures using a derivative estimator

NASA Astrophysics Data System (ADS)

Chesne, Simon

2014-12-01

This paper proposes a new method for the identification of boundary forces (shear force or bending moment) in a beam, based on displacement measurements. The problem is considered in terms of the determination of the boundary spatial derivatives of transverse displacements. By assuming the displacement fields to be approximated by Taylor expansions in a domain close to the boundaries, the spatial derivatives can be estimated using specific point-wise derivative estimators. This approach makes it possible to extract the derivatives using a weighted spatial integration of the displacement field. Following the theoretical description, numerical simulations made with exact and noisy data are used to determine the relationship between the size of the integration domain and the wavelength of the vibrations. The simulations also highlight the self-regularization of the technique. Experimental measurements demonstrate the feasibility and accuracy of the proposed method.

Atomic force microscopy studies on molybdenum disulfide flakes as sodium-ion anodes.

PubMed

Lacey, Steven D; Wan, Jiayu; von Wald Cresce, Arthur; Russell, Selena M; Dai, Jiaqi; Bao, Wenzhong; Xu, Kang; Hu, Liangbing

2015-02-11

A microscale battery comprised of mechanically exfoliated molybdenum disulfide (MoS2) flakes with copper connections and a sodium metal reference was created and investigated as an intercalation model using in situ atomic force microscopy in a dry room environment. While an ethylene carbonate-based electrolyte with a low vapor pressure allowed topographical observations in an open cell configuration, the planar microbattery was used to conduct in situ measurements to understand the structural changes and the concomitant solid electrolyte interphase (SEI) formation at the nanoscale. Topographical observations demonstrated permanent wrinkling behavior of MoS2 electrodes upon sodiation at 0.4 V. SEI formation occurred quickly on both flake edges and planes at voltages before sodium intercalation. Force spectroscopy measurements provided quantitative data on the SEI thickness for MoS2 electrodes in sodium-ion batteries for the first time.

Cellular Tug-of-War: Forces at Work and DNA Stretching in Mitosis

NASA Astrophysics Data System (ADS)

Griffin, Brian; Kilfoil, Maria L.

2013-03-01

In the microscopic world of the cell dominated by thermal noise, a cell must be able to successfully segregate its DNA with high fidelity in order to pass its genetic information on to its progeny. In this process of mitosis in eukaryotes, driving forces act on the cytoskeleton-based architecture called the mitotic spindle to promote this division. Our preliminary data demonstrates that the dynamics of this process in yeast cells is universal. Moreover, the dynamics suggest an increasing load as the chromosomes are pulled apart. To investigate this, we use three-dimensional imaging to track the dynamics of the poles of this architecture and the points of attachment to chromosomes simultaneously and with high spatial resolution. We analyze the relative motions of chromosomes as they are organized before segregation and as they are pulled apart, using this data to investigate the force-response behavior of this cytoskeleton-chromosome polymer system.

Rigorous force field optimization principles based on statistical distance minimization

DOE PAGES

Vlcek, Lukas; Chialvo, Ariel A.

2015-10-12

We use the concept of statistical distance to define a measure of distinguishability between a pair of statistical mechanical systems, i.e., a model and its target, and show that its minimization leads to general convergence of the model’s static measurable properties to those of the target. Here we exploit this feature to define a rigorous basis for the development of accurate and robust effective molecular force fields that are inherently compatible with coarse-grained experimental data. The new model optimization principles and their efficient implementation are illustrated through selected examples, whose outcome demonstrates the higher robustness and predictive accuracy of themore » approach compared to other currently used methods, such as force matching and relative entropy minimization. We also discuss relations between the newly developed principles and established thermodynamic concepts, which include the Gibbs-Bogoliubov inequality and the thermodynamic length.« less

Effect of tool geometry and cutting parameters on delamination and thrust forces in drilling CFRP/Al-Li

NASA Astrophysics Data System (ADS)

El Bouami, Souhail; Habak, Malek; Franz, Gérald; Velasco, Raphaël; Vantomme, Pascal

2016-10-01

Composite materials are increasingly used for structural parts in the aeronautic industries. Carbon Fiber-Reinforced Plastics (CFRP) are often used in combination with metallic materials, mostly aluminium alloys. This raises new problems in aircraft assembly. Delamination is one of these problems. In this study, CFRP/Al-Li stacks is used as experimental material for investigation effect of interaction of cutting parameters (cutting speed and feed rate) and tool geometry on delamination and thrust forces in drilling operation. A plan of experiments, based on Taguchi design method, was employed to investigate the influence of tool geometry and in particular the point angle and cutting parameters on delamination and axial effort. The experimental results demonstrate that the feed rate is the major parameter and the importance of tool point angle in delamination and thrust forces in the stacks were shown.

The effect of changing wind forcing on Antarctic ice shelf melting in high-resolution, global sea ice-ocean simulations with the Accelerated Climate Model for Energy (ACME)

NASA Astrophysics Data System (ADS)

Asay-Davis, Xylar; Price, Stephen; Petersen, Mark; Wolfe, Jonathan

2017-04-01

The capability for simulating sub-ice shelf circulation and submarine melting and freezing has recently been added to the U.S. Department of Energy's Accelerated Climate Model for Energy (ACME). With this new capability, we use an eddy permitting ocean model to conduct two sets of simulations in the spirit of Spence et al. (GRL, 41, 2014), who demonstrate increased warm water upwelling along the Antarctic coast in response to poleward shifting and strengthening of Southern Ocean westerly winds. These characteristics, symptomatic of a positive Southern Annular Mode (SAM), are projected to continue into the 21st century under anthropogenic climate change (Fyfe et al., J. Clim., 20, 2007). In our first simulation, we force the climate model using the standard CORE interannual forcing dataset (Large and Yeager; Clim. Dyn., 33, 2009). In our second simulation, we force our climate model using an altered version of CORE interannual forcing, based on the latter half of the full time series, which we take as a proxy for a future climate state biased towards a positive SAM. We compare ocean model states and sub-ice shelf melt rates with observations, exploring sources of model biases as well as the effects of the two forcing scenarios.

An MRI-Guided Telesurgery System Using a Fabry-Perot Interferometry Force Sensor and a Pneumatic Haptic Device.

PubMed

Su, Hao; Shang, Weijian; Li, Gang; Patel, Niravkumar; Fischer, Gregory S

2017-08-01

This paper presents a surgical master-slave teleoperation system for percutaneous interventional procedures under continuous magnetic resonance imaging (MRI) guidance. The slave robot consists of a piezoelectrically actuated 6-degree-of-freedom (DOF) robot for needle placement with an integrated fiber optic force sensor (1-DOF axial force measurement) using the Fabry-Perot interferometry (FPI) sensing principle; it is configured to operate inside the bore of the MRI scanner during imaging. By leveraging the advantages of pneumatic and piezoelectric actuation in force and position control respectively, we have designed a pneumatically actuated master robot (haptic device) with strain gauge based force sensing that is configured to operate the slave from within the scanner room during imaging. The slave robot follows the insertion motion of the haptic device while the haptic device displays the needle insertion force as measured by the FPI sensor. Image interference evaluation demonstrates that the telesurgery system presents a signal to noise ratio reduction of less than 17% and less than 1% geometric distortion during simultaneous robot motion and imaging. Teleoperated needle insertion and rotation experiments were performed to reach 10 targets in a soft tissue-mimicking phantom with 0.70 ± 0.35 mm Cartesian space error.

Reducing detrimental electrostatic effects in Casimir-force measurements and Casimir-force-based microdevices

NASA Astrophysics Data System (ADS)

Xu, Jun; Klimchitskaya, G. L.; Mostepanenko, V. M.; Mohideen, U.

2018-03-01

It is well known that residual electrostatic forces create significant difficulties in precise measurements of the Casimir force and the wide use of Casimir-operated microdevices. We experimentally demonstrate that, with the help of Ar-ion cleaning of the surfaces, it is possible to make electrostatic effects negligibly small compared to the Casimir interaction. Our experimental setup consists of a dynamic atomic force microscope supplemented with an Ar-ion gun and argon reservoir. The residual potential difference between the Au-coated surfaces of a sphere and those of a plate was measured both before and after in situ Ar-ion cleaning. It is shown that this cleaning decreases the magnitude of the residual potential by up to an order of magnitude and makes it almost independent of the separation. The gradient of the Casimir force was measured using ordinary samples subjected to Ar-ion cleaning. The obtained results are shown to be in good agreement both with previous precision measurements using specially selected samples and with theoretical predictions of the Lifshitz theory. The conclusion is made that the suggested method of in situ Ar-ion cleaning is effective in reducing the electrostatic effects and therefore is a great resource for experiments on measuring the Casimir interaction and for Casimir-operated microdevices.

Optical force on a large sphere illuminated by Bessel beams: comparisons between ray optics method and generalized Lorenz-Mie theory.

PubMed

Song, Shukun; Wang, Neng; Lu, Wanli; Lin, Zhifang

2014-10-01

Optical forces are calculated for a dielectric spherical particle illuminated by a zero-order Bessel beam based on both the generalized Lorenz-Mie theory (GLMT) and the ray optics method (ROM). Particles with positive and negative refractive indices are examined. The peculiar characteristics of the Bessel beam allow for analytical expressions for the beam shape coefficients required in the GLMT as well as a decomposition of optical force into the gradient and the scattering forces irrespective of the particle size, which enable respective comparisons for the gradient and scattering forces between the results obtained from the GLMT and the ROM. Our results demonstrate that the discrepancy between the results obtained from the GLMT and the ROM depends on the particle refractive index n_p, the particle size, and, also, the particle location in the beam field. As the particle size increases, the difference between the results from the GLMT and the ROM shows a general tendency of decreasing, as can be expected, but the change may exhibit oscillatory rather than monotonic behavior. A phase diagram is presented that displays the regime for particle size and refractive index where a specified accuracy can be achieved for optical force by the ROM.

A Catchment-Based Approach to Modeling Land Surface Processes in a GCM. Part 2; Parameter Estimation and Model Demonstration

NASA Technical Reports Server (NTRS)

Ducharne, Agnes; Koster, Randal D.; Suarez, Max J.; Stieglitz, Marc; Kumar, Praveen

2000-01-01

The viability of a new catchment-based land surface model (LSM) developed for use with general circulation models is demonstrated. First, simple empirical functions -- tractable enough for operational use in the LSM -- are established that faithfully capture the control of topography on the subgrid variability of soil moisture and the surface water budget, as predicted by theory. Next, the full LSM is evaluated offline. Using forcing and validation datasets developed for PILPS Phase 2c, the minimally calibrated model is shown to reproduce observed evaporation and runoff fluxes successfully in the Red-Arkansas River Basin. A complementary idealized study that employs the range of topographic variability seen over North America demonstrates that the simulated surface water budget does vary strongly with topography, which can, by itself, induce variations in annual evaporation as high as 20%.

Objective Integrated Assessment of Functional Outcomes in Reduction Mammaplasty

PubMed Central

Passaro, Ilaria; Malovini, Alberto; Faga, Angela; Toffola, Elena Dalla

2013-01-01

Background: The aim of our study was an objective integrated assessment of the functional outcomes of reduction mammaplasty. Methods: The study involved 17 women undergoing reduction mammaplasty from March 2009 to June 2011. Each patient was assessed before surgery and 2 months postoperatively with the original association of 4 subjective and objective assessment methods: a physiatric clinical examination, the Roland Morris Disability Questionnaire, the Berg Balance Scale, and a static force platform analysis. Results: All of the tests proved multiple statistically significant associated outcomes demonstrating a significant improvement in the functional status following reduction mammaplasty. Surgical correction of breast hypertrophy could achieve both spinal pain relief and recovery of performance status in everyday life tasks, owing to a muscular postural functional rearrangement with a consistent antigravity muscle activity sparing. Pain reduction in turn could reduce the antalgic stiffness and improved the spinal range of motion. In our sample, the improvement of the spinal range of motion in flexion matched a similar improvement in extension. Recovery of a more favorable postural pattern with reduction of the anterior imbalance was demonstrated by the static force stabilometry. Therefore, postoperatively, all of our patients narrowed the gap between the actual body barycenter and the ideal one. The static force platform assessment also consistently confirmed the effectiveness of an accurate clinical examination of functional impairment from breast hypertrophy. Conclusions: The static force platform assessment might help the clinician to support the diagnosis of functional impairment from a breast hypertrophy with objectively based data. PMID:25289256

Stratospheric sulfur and its implications for radiative forcing simulated by the chemistry climate model EMAC

PubMed Central

Brühl, C; Lelieveld, J; Tost, H; Höpfner, M; Glatthor, N

2015-01-01

Multiyear simulations with the atmospheric chemistry general circulation model EMAC with a microphysical modal aerosol module at high vertical resolution demonstrate that the sulfur gases COS and SO2, the latter from low-latitude and midlatitude volcanic eruptions, predominantly control the formation of stratospheric aerosol. Marine dimethyl sulfide (DMS) and other SO2 sources, including strong anthropogenic emissions in China, are found to play a minor role except in the lowermost stratosphere. Estimates of volcanic SO2 emissions are based on satellite observations using Total Ozone Mapping Spectrometer and Ozone Monitoring Instrument for total injected mass and Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) on Envisat or Stratospheric Aerosol and Gases Experiment for the spatial distribution. The 10 year SO2 and COS data set of MIPAS is also used for model evaluation. The calculated radiative forcing of stratospheric background aerosol including sulfate from COS and small contributions by DMS oxidation, and organic aerosol from biomass burning, is about 0.07W/m2. For stratospheric sulfate aerosol from medium and small volcanic eruptions between 2005 and 2011 a global radiative forcing up to 0.2W/m2 is calculated, moderating climate warming, while for the major Pinatubo eruption the simulated forcing reaches 5W/m2, leading to temporary climate cooling. The Pinatubo simulation demonstrates the importance of radiative feedback on dynamics, e.g., enhanced tropical upwelling, for large volcanic eruptions. PMID:25932352

Robot-assisted adaptive training: custom force fields for teaching movement patterns.

PubMed

Patton, James L; Mussa-Ivaldi, Ferdinando A

2004-04-01

Based on recent studies of neuro-adaptive control, we tested a new iterative algorithm to generate custom training forces to "trick" subjects into altering their target-directed reaching movements to a prechosen movement as an after-effect of adaptation. The prechosen movement goal, a sinusoidal-shaped path from start to end point, was never explicitly conveyed to the subject. We hypothesized that the adaptation would cause an alteration in the feedforward command that would result in the prechosen movement. Our results showed that when forces were suddenly removed after a training period of 330 movements, trajectories were significantly shifted toward the prechosen movement. However, de-adaptation occurred (i.e., the after-effect "washed out") in the 50-75 movements that followed the removal of the training forces. A second experiment suppressed vision of hand location and found a detectable reduction in the washout of after-effects, suggesting that visual feedback of error critically influences learning. A final experiment demonstrated that after-effects were also present in the neighborhood of training--44% of original directional shift was seen in adjacent, unpracticed movement directions to targets that were 60 degrees different from the targets used for training. These results demonstrate the potential for these methods for teaching motor skills and for neuro-rehabilitation of brain-injured patients. This is a form of "implicit learning," because unlike explicit training methods, subjects learn movements with minimal instructions, no knowledge of, and little attention to the trajectory.

Ponderomotive Forces in Cosmos

NASA Astrophysics Data System (ADS)

Lundin, R.; Guglielmi, A.

2006-12-01

This review is devoted to ponderomotive forces and their importance for the acceleration of charged particles by electromagnetic waves in space plasmas. Ponderomotive forces constitute time-averaged nonlinear forces acting on a media in the presence of oscillating electromagnetic fields. Ponderomotive forces represent a useful analytical tool to describe plasma acceleration. Oscillating electromagnetic fields are also related with dissipative processes, such as heating of particles. Dissipative processes are, however, left outside these discussions. The focus will be entirely on the (conservative) ponderomotive forces acting in space plasmas. The review consists of seven sections. In Section 1, we explain the rational for using the auxiliary ponderomotive forces instead of the fundamental Lorentz force for the study of particle motions in oscillating fields. In Section 2, we present the Abraham, Miller, Lundin-Hultqvist and Barlow ponderomotive forces, and the Bolotovsky-Serov ponderomotive drift. The hydrodynamic, quasi-hydrodynamic, and ‘`test-particle’' approaches are used for the study of ponderomotive wave-particle interaction. The problems of self-consistency and regularization are discussed in Section 3. The model of static balance of forces (Section 4) exemplifies the interplay between thermal, gravitational and ponderomotive forces, but it also introduces a set of useful definitions, dimensionless parameters, etc. We analyze the Alfvén and ion cyclotron waves in static limit with emphasis on the specific distinction between traveling and standing waves. Particular attention has been given to the impact of traveling Alfvén waves on the steady state anabatic wind that blows over the polar regions (Section~5). We demonstrate the existence of a wave-induced cold anabatic wind. We also show that, at a critical point, the ponderomotive acceleration of the wind is a factor of 3 greater than the thermal acceleration. Section 6 demonstrates various manifestations of ponderomotive forces in the Earth's magnetosphere, for instance the ionospheric plasma acceleration and outflow. The polar wind and the auroral density cavities are considered in relation to results from the Freja and Viking satellites. The high-altitude energization and escape of ions is discussed. The ponderomotive anharmonicity of standing Alfvén waves is analyzed from ground based ULF wave measurements. The complexity of the many challenging problems related with plasma processes near the magnetospheric boundaries is discussed in the light of recent Cluster observations. At the end of Section 6, we consider the application of ponderomotive forces to the diversity of phenomena on the Sun, in the interstellar environment, on newborn stars, pulsars and active galaxies. We emphasize the role of forcing of magnetized plasmas in general and ponderomotive forcing in particular, presenting some simple conceivable scenarios for massive outflow and jets from astrophysical objects.

Evidence-based Assessment of Cognitive Functioning in Pediatric Psychology

PubMed Central

Brown, Ronald T.; Cavanagh, Sarah E.; Vess, Sarah F.; Segall, Mathew J.

2008-01-01

Objective To review the evidence base for measures of cognitive functioning frequently used within the field of pediatric psychology. Methods From a list of 47 measures identified by the Society of Pediatric Psychology (Division 54) Evidence-Based Assessment Task Force Workgroup, 27 measures were included in the review. Measures were organized, reviewed, and evaluated according to general domains of functioning (e.g., attention/executive functioning, memory). Results Twenty-two of 27 measures reviewed demonstrated psychometric properties that met “Well-established” criteria as set forth by the Assessment Task Force. Psychometric properties were strongest for measures of general cognitive ability and weakest for measures of visual-motor functioning and attention. Conclusions We report use of “Well-established” measures of overall cognitive functioning, nonverbal intelligence, academic achievement, language, and memory and learning. For several specific tests in the domains of visual-motor functioning and attention, additional psychometric data are needed for measures to meet criteria as “Well established.” PMID:18194973

Atomic Force Microscopy Based Cell Shape Index

NASA Astrophysics Data System (ADS)

Adia-Nimuwa, Usienemfon; Mujdat Tiryaki, Volkan; Hartz, Steven; Xie, Kan; Ayres, Virginia

2013-03-01

Stellation is a measure of cell physiology and pathology for several cell groups including neural, liver and pancreatic cells. In the present work, we compare the results of a conventional two-dimensional shape index study of both atomic force microscopy (AFM) and fluorescent microscopy images with the results obtained using a new three-dimensional AFM-based shape index similar to sphericity index. The stellation of astrocytes is investigated on nanofibrillar scaffolds composed of electrospun polyamide nanofibers that has demonstrated promise for central nervous system (CNS) repair. Recent work by our group has given us the ability to clearly segment the cells from nanofibrillar scaffolds in AFM images. The clear-featured AFM images indicated that the astrocyte processes were longer than previously identified at 24h. It was furthermore shown that cell spreading could vary significantly as a function of environmental parameters, and that AFM images could record these variations. The new three-dimensional AFM-based shape index incorporates the new information: longer stellate processes and cell spreading. The support of NSF PHY-095776 is acknowledged.

Network community-based model reduction for vortical flows

NASA Astrophysics Data System (ADS)

Gopalakrishnan Meena, Muralikrishnan; Nair, Aditya G.; Taira, Kunihiko

2018-06-01

A network community-based reduced-order model is developed to capture key interactions among coherent structures in high-dimensional unsteady vortical flows. The present approach is data-inspired and founded on network-theoretic techniques to identify important vortical communities that are comprised of vortical elements that share similar dynamical behavior. The overall interaction-based physics of the high-dimensional flow field is distilled into the vortical community centroids, considerably reducing the system dimension. Taking advantage of these vortical interactions, the proposed methodology is applied to formulate reduced-order models for the inter-community dynamics of vortical flows, and predict lift and drag forces on bodies in wake flows. We demonstrate the capabilities of these models by accurately capturing the macroscopic dynamics of a collection of discrete point vortices, and the complex unsteady aerodynamic forces on a circular cylinder and an airfoil with a Gurney flap. The present formulation is found to be robust against simulated experimental noise and turbulence due to its integrating nature of the system reduction.

Evaluation of adhesion force between functionalized microbeads and protein-coated stainless steel using shear-flow-induced detachment.

PubMed

Mercier-Bonin, Muriel; Adoue, Mathieu; Zanna, Sandrine; Marcus, Philippe; Combes, Didier; Schmitz, Philippe

2009-10-01

Spherical microbeads functionalized with two types of chemical groups (NH(2), OH) were chosen as a simplified bacterial model, in order to elucidate the role of macromolecular interactions between specific biopolymers and 316 L stainless steel, in the frame of biofilm formation in the marine environment. NH(2) microbeads were used in their native form or after covalent binding to BSA or different representative poly-amino acids. OH microbeads were used in their native form. Adhesion force between microbeads and bare or BSA-coated stainless steel was quantified at nanoscale. Shear-flow-induced detachment experiments were combined with a simplified version of a theoretical model, based on the balance of hydrodynamic forces and torque exerted on microbeads. A maximal adhesion force of 27.6+/-8.5 nN was obtained for BSA-coated NH(2) microbeads. The high reactivity of OH functional groups was assessed (adhesion force of 15.6+/-4.8 nN for large microbeads). When charge-conducting stainless steel was coated with BSA, adhesion force was significantly lower than the one estimated with the bare surface, probably due to an increase in hydrophilic surface properties or suppression of charge transfer. The mechanism for microbead detachment was established (mainly rolling). The flow chamber and the associated theoretical modelling were demonstrated to be a relevant approach to quantify nanoscale forces between interacting surfaces.

Exploring the effects of dimensionality reduction in deep networks for force estimation in robotic-assisted surgery

NASA Astrophysics Data System (ADS)

Aviles, Angelica I.; Alsaleh, Samar; Sobrevilla, Pilar; Casals, Alicia

2016-03-01

Robotic-Assisted Surgery approach overcomes the limitations of the traditional laparoscopic and open surgeries. However, one of its major limitations is the lack of force feedback. Since there is no direct interaction between the surgeon and the tissue, there is no way of knowing how much force the surgeon is applying which can result in irreversible injuries. The use of force sensors is not practical since they impose different constraints. Thus, we make use of a neuro-visual approach to estimate the applied forces, in which the 3D shape recovery together with the geometry of motion are used as input to a deep network based on LSTM-RNN architecture. When deep networks are used in real time, pre-processing of data is a key factor to reduce complexity and improve the network performance. A common pre-processing step is dimensionality reduction which attempts to eliminate redundant and insignificant information by selecting a subset of relevant features to use in model construction. In this work, we show the effects of dimensionality reduction in a real-time application: estimating the applied force in Robotic-Assisted Surgeries. According to the results, we demonstrated positive effects of doing dimensionality reduction on deep networks including: faster training, improved network performance, and overfitting prevention. We also show a significant accuracy improvement, ranging from about 33% to 86%, over existing approaches related to force estimation.

Quantification of in-contact probe-sample electrostatic forces with dynamic atomic force microscopy.

PubMed

Balke, Nina; Jesse, Stephen; Carmichael, Ben; Okatan, M Baris; Kravchenko, Ivan I; Kalinin, Sergei V; Tselev, Alexander

2017-01-04

Atomic force microscopy (AFM) methods utilizing resonant mechanical vibrations of cantilevers in contact with a sample surface have shown sensitivities as high as few picometers for detecting surface displacements. Such a high sensitivity is harnessed in several AFM imaging modes. Here, we demonstrate a cantilever-resonance-based method to quantify electrostatic forces on a probe in the probe-sample junction in the presence of a surface potential or when a bias voltage is applied to the AFM probe. We find that the electrostatic forces acting on the probe tip apex can produce signals equivalent to a few pm of surface displacement. In combination with modeling, the measurements of the force were used to access the strength of the electrical field at the probe tip apex in contact with a sample. We find an evidence that the electric field strength in the junction can reach ca. 1 V nm -1 at a bias voltage of a few volts and is limited by non-ideality of the tip-sample contact. This field is sufficiently strong to significantly influence material states and kinetic processes through charge injection, Maxwell stress, shifts of phase equilibria, and reduction of energy barriers for activated processes. Besides, the results provide a baseline for accounting for the effects of local electrostatic forces in electromechanical AFM measurements as well as offer additional means to probe ionic mobility and field-induced phenomena in solids.

Improved treatment of nucleosides and nucleotides in the OPLS-AA force field

NASA Astrophysics Data System (ADS)

Robertson, Michael J.; Tirado-Rives, Julian; Jorgensen, William L.

2017-09-01

DFT calculations have been used to develop improved descriptions of the torsional energetics for nucleosides and nucleotides in the OPLS-AA force field. Scans of nucleotide dihedral angles (γ, χ, and β) and methyl phosphates provided the bases for the new torsional parameters. In addition, the angle-bending parameters of phosphodiesters and ribose were updated, and adjustments were made to existing carbohydrate torsions to better capture the sugar puckering landscape of ribose. MD simulations of nucleosides with the new parameters demonstrate a significant improvement in the ribose sugar puckering and χ angle distributions. Additionally, energy-minimization of protein-nucleotide crystal structures with the new parameters produced accurate poses.

The effect of the London-van der Waals dispersion force on interline heat transfer

NASA Technical Reports Server (NTRS)

Wayner, P. C., Jr.

1978-01-01

A theoretical procedure to determine the heat transfer characteristics of the interline region (junction of liquid-solid-vapor) from the macroscopic optical and thermophysical properties of the system is outlined. The analysis is based on the premise that the interline transport processes are controlled by the London-van der Waals dispersion force between condensed phases (solid and liquid). Numerical values of the dispersion constant are presented. The procedure is used to compare the relative size of the interline heat sink of various systems using a constant heat flux mode. This solution demonstrates the importance of the interline heat flow number, which is evaluated for various systems.

Induced movement of the magnetic beads and DNA-based dumbbell in a micro fluidic channel

NASA Astrophysics Data System (ADS)

Babić, B.; Ghai, R.; Dimitrov, K.

2007-12-01

We have explored controlled movement of magnetic beads and a dumbbell structure composed of DNA, a magnetic and a non-magnetic bead in a micro fluidic channel. Movement of the beads and dumbbells is simulated assuming that a net force is described as a superposition between the magnetic and hydrodynamic drag forces. Trajectories of beads and dumbbells are observed with optical light microscopy. The experimentally measured data show a good agreement with the simulations. This dynamical approach offers the prospect to stretch the DNA within the dumbbell and investigate its conformational changes. Further on, we demonstrate that short sonication can reduce multiple attachments of DNA to the beads.

Theory of the locomotion of nematodes

PubMed Central

Niebur, Ernst; Erdös, Paul

1991-01-01

We develop a model of the undulatory locomotion of nematodes, in particular that of Caenorhabditis elegans, based on mechanics. The model takes into account the most important forces acting on a moving worm and allows the computer simulation of a creeping nematode. These forces are produced by the interior pressure in the liquid-filled body cavity, the elasticity of the cuticle, the excitation of certain sets of muscles and the friction between the body and its support. We propose that muscle excitation patterns can be generated by stretch receptor control. By solving numerically the equations of motion of the model of the nematode, we demonstrate that these muscle excitation patterns are suitable for the propulsion of the animal. PMID:19431807