Environmental Assessment for Renovation and Small Addition for AGE Facility MacDill AFB, Florida

DTIC Science & Technology

2005-05-01

one lift station, all of the sanitary sewer lines have, in essence , at least one check valve to stop the inflow of floodwater to the WWTP. The...Force Base Installation Restoration Program 7621 Hillsborough Loop Dr. MacDill AFB, FL 33621-5207 Jasmine Raffington FL Coastal Management

Photocopy of drawing (original drawing of War Department Theater in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of War Department Theater in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General)STRUCTURAL - FOUNDATIONS - MacDill Air Force Base, Base Theater, 2420 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of War Department Theater in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of War Department Theater in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) EXTERIOR ELEVATIONS - MacDill Air Force Base, Base Theater, 2420 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of MacDill Field in possession ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of MacDill Field in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1952 architectural drawings by Strategic Air Command, MacDill Air Force Base) BASE LAYOUT, 1952 - MacDill Air Force Base, Bounded by City of Tampa North, Tampa Bay South, Old Tampa Bay West, & Hillsborough Bay East, Tampa, Hillsborough County, FL

Final Environmental Assessment: For Construction of a Security Forces Complex on Eglin Air Force Base, FL

DTIC Science & Technology

2005-05-01

form of weapons cleaning products and wastes. State of Florida and Air Force regulations have been implemented 05/31/05 Final Environmental Assessment...Forces Complex will generate hazardous materials in the form of weapons cleaning products and wastes. Break-Free CLP Liquid is a cleaner, lubricant

Photocopy of drawing (original drawing of War Department Theater in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of War Department Theater in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) FIRST FLOOR AND CEILING PLAN - MacDill Air Force Base, Base Theater, 2420 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of War Department Theater in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of War Department Theater in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) FLOOR PLAN AND BENCH DETAILS - MacDill Air Force Base, Base Theater, 2420 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Signal & Ordnance Warehouse ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Signal & Ordnance Warehouse in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General)TRUSS DETAILS - MacDill Air Force Base, Signal & Ordnance Warehouse, 7620 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of print (original sepia print is backward and in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of print (original sepia print is backward and in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1953 architectural drawings by Horowick & Lee, Architects, Jacksonville, Florida) EXTERIOR ELEVATIONS - MacDill Air Force Base, Photography Laboratory, 2617 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of print (original sepia print is backward and in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of print (original sepia print is backward and in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1953 architectural drawings by Horowick & Lee, Architects, Jacksonville, Florida) FLOOR PLAN AND SCHEDULES - MacDill Air Force Base, Photography Laboratory, 2617 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Photographic Laboratory Building in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Photographic Laboratory Building in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1939 architectural drawings by Construction Division, Office of the Quartermaster General) PLANS - MacDill Air Force Base, Photographic Laboratory, 7718 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of MacDill Field in possession ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of MacDill Field in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; site plan dated December, 1942) BASE LAYOUT, DECEMBER 1942 - MacDill Air Force Base, Bounded by City of Tampa North, Tampa Bay South, Old Tampa Bay West, & Hillsborough Bay East, Tampa, Hillsborough County, FL

Photocopy of drawing (original blueprint of Special Type Service Station ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original blueprint of Special Type Service Station in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1941 architectural drawings by Standard Oil Company Engineering Department of Louisville, KY) ELEVATIONS & SECTION - MacDill Air Force Base, Service Station, 7303 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original blueprint of Special Type Service Station ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original blueprint of Special Type Service Station in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1941 architectural drawings by Standard Oil Company Engineering Department of Louisville, KY) PLAN - MacDill Air Force Base, Service Station, 7303 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original blueprint of Special Type Service Station ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original blueprint of Special Type Service Station in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1941 architectural drawings by Standard Oil Company Engineering Department of Louisville, KY) SITE PLAN - MacDill Air Force Base, Service Station, 7303 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Signal & Ordnance Warehouse ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Signal & Ordnance Warehouse in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) SECTIONS AND DETAILS - MacDill Air Force Base, Signal & Ordnance Warehouse, 7620 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) END ELEVATION AND SECTIONS - MacDill Air Force Base, Quartermaster Warehouse & Commissary, 7621 Hillsborough Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Photographic Laboratory Building in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Photographic Laboratory Building in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1939 architectural drawings by Construction Division, Office of the Quartermaster General) MAIN ENTRANCE DETAILS - MacDill Air Force Base, Photographic Laboratory, 7718 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of PaintOil & Dope Building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Paint-Oil & Dope Building in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) DETAILS - MacDill Air Force Base, Paint, Oil & Dope Building, 7716 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) SECTIONS AND DETAILS - MacDill Air Force Base, Quartermaster Warehouse & Commissary, 7621 Hillsborough Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of PaintOil & Dope Building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Paint-Oil & Dope Building in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) PLAN AND ELEVATIONS - MacDill Air Force Base, Paint, Oil & Dope Building, 7716 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Signal & Ordnance Warehouse ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Signal & Ordnance Warehouse in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) PLANS, ELEVATIONS, SECTIONS AND DETAILS - MacDill Air Force Base, Signal & Ordnance Warehouse, 7620 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) FRONT AND REAR ELEVATIONS - MacDill Air Force Base, Quartermaster Warehouse & Commissary, 7621 Hillsborough Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Double N.C.O. Quarters in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Double N.C.O. Quarters in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1939 architectural drawings by Construction Division, Office of the Quartermaster General) INTERIOR DETAILS - MacDill Air Force Base, Double Non-Commissioned Officers' Quarters, 7418 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Armament & Instrument Inspection ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Armament & Instrument Inspection and Adjustment Bldg. in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1941 architectural drawings by Construction Division, Office of the Quartermaster General) ELEVATIONS AND DETAILS - MacDill Air Force Base, Armament & Instrument Inspection & Adjustment Building, 7807 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Q.M. Gas & Oil ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Q.M. Gas & Oil House in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) ELEVATIONS AND DETAILS - MacDill Air Force Base, Quartermaster Gas & Oil House, 8103 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Armament & Instrument Inspection ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Armament & Instrument Inspection and Adjustment Bldg. in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1941 architectural drawings by Construction Division, Office of the Quartermaster General) ELEVATIONS AND SECTIONS - MacDill Air Force Base, Armament & Instrument Inspection & Adjustment Building, 7807 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Double N.C.O. Quarters in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Double N.C.O. Quarters in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1939 architectural drawings by Construction Division, Office of the Quartermaster General) ELEVATIONS AND SECTION - MacDill Air Force Base, Double Non-Commissioned Officers' Quarters, 7418 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Fire and Guard House ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Fire and Guard House with Communication Center in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) ELEVATIONS AND SECTIONS - MacDill Air Force Base, Fire & Guard House, 2709 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Photographic Laboratory Building in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Photographic Laboratory Building in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1939 architectural drawings by Construction Division, Office of the Quartermaster General) FRONT AND RIGHT SIDE ELEVATIONS AND SECTIONS - MacDill Air Force Base, Photographic Laboratory, 7718 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Fire and Guard House ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Fire and Guard House with Communication Center in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) EXTERIOR AND MISCELLANEOUS DETAILS - MacDill Air Force Base, Fire & Guard House, 2709 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Photographic Laboratory Building in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Photographic Laboratory Building in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1939 architectural drawings by Construction Division, Office of the Quartermaster General) REAR AND LEFT SIDE ELEVATIONS AND SECTIONS - MacDill Air Force Base, Photographic Laboratory, 7718 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Fire and Guard House ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Fire and Guard House with Communication Center in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) FRONT ELEVATION DETAILS - MacDill Air Force Base, Fire & Guard House, 2709 Florida Keys Avenue, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Sewage Treatment Plant ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Sewage Treatment Plant - No. 1 Pump House in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) ELEVATIONS, SECTIONS, AND DETAILS - MacDill Air Force Base, Pump House No. 1, Hillsborough Garden Drive & Tampa Boulevard, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Q.M. Warehouse & Commissary in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) FIRST FLOOR PLAN AND DOOR DETAILS - MacDill Air Force Base, Quartermaster Warehouse & Commissary, 7621 Hillsborough Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Q.M. Warehouse in possession ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Q.M. Warehouse in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) PLANS, ELEVATIONS, SECTIONS, AND ELECTRICAL DETAILS - MacDill Air Force Base, Quartermaster Warehouse, 7605 Hillsborough Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Sewage Treatment Plant ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Sewage Treatment Plant - No. 1 Pump House in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) FLOOR PLANS AND SECTIONS - MacDill Air Force Base, Pump House No. 1, Hillsborough Garden Drive & Tampa Boulevard, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Fire and Guard House ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Fire and Guard House with Communication Center in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) CELL BLOCK DETAILS - MacDill Air Force Base, Fire & Guard House, 2709 Florida Keys Avenue, Tampa, Hillsborough County, FL

Self-induced pinning of vortices in the presence of ac driving force in magnetic superconductors

NASA Astrophysics Data System (ADS)

Bulaevskii, Lev N.; Lin, Shi-Zeng

2012-12-01

We derive the response of the magnetic superconductors in the vortex state to the ac Lorentz force, FL(t)=Facsin(ωt), taking into account the interaction of vortices with the magnetic moments described by the relaxation dynamics (polaronic effect). At low amplitudes of the driving force Fac the dissipation in the system is suppressed due to the enhancement of the effective viscosity at low frequencies and due to formation of the magnetic pinning at high frequencies ω. In the adiabatic limit with low frequencies ω and high amplitude of the driving force Fac, the vortex and magnetic polarization form a vortex polaron when FL(t) is small. When FL increases, the vortex polaron accelerates and at a threshold driving force, the vortex polaron dissociates and the motion of vortex and the relaxation of magnetization are decoupled. When FL decreases, the vortex is retrapped by the background of remnant magnetization and they again form vortex polaron. This process repeats when FL(t) increases in the opposite direction. Remarkably, after dissociation, decoupled vortices move in the periodic potential induced by magnetization which remains for some periods of time due to retardation after the decoupling. At this stage vortices oscillate with high frequencies determined by the Lorentz force at the moment of dissociation. We derive also the creep rate of vortices and show that magnetic moments suppress creep rate.

75 FR 28752 - Amendment and Establishment of Restricted Areas and Other Special Use Airspace, Avon Park Air...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-24

... Other Special Use Airspace, Avon Park Air Force Range, FL AGENCY: Federal Aviation Administration (FAA... Avon Park Air Force Range Joint Land Use Study, sponsored by the Central Florida Regional Planning... reviewed the Avon Park Air Force Range, FL, Special Use Airspace modifications and has determined that the...

Photocopy of drawing (original drawing of Armament & Instrument Inspection ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Armament & Instrument Inspection and Adjustment Bldg. in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1941 architectural drawings by Construction Division, Office of the Quartermaster General) FIRST FLOOR PLAN, SECTIONS, AND DETAILS - MacDill Air Force Base, Armament & Instrument Inspection & Adjustment Building, 7807 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Double N.C.O. Quarters in ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Double N.C.O. Quarters in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1939 architectural drawings by Construction Division, Office of the Quartermaster General) FIRST AND SECOND FLOOR PLANS - MacDill Air Force Base, Double Non-Commissioned Officers' Quarters, 7418 Hanger Loop Drive, Tampa, Hillsborough County, FL

Photocopy of drawing (original drawing of Fire and Guard House ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Fire and Guard House with Communication Center in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1940 architectural drawings by Construction Division, Office of the Quartermaster General) FIRST AND SECOND FLOOR AND ROOF PLANS - MacDill Air Force Base, Fire & Guard House, 2709 Florida Keys Avenue, Tampa, Hillsborough County, FL

33 CFR 334.595 - Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape Canaveral Air Force Station, FL...

Code of Federal Regulations, 2014 CFR

2014-07-01

...; 45th Space Wing, Cape Canaveral Air Force Station, FL; restricted area. 334.595 Section 334.595.... The regulations in this section shall be enforced by the Commander, 45th Space Wing, Patrick Air Force... AND RESTRICTED AREA REGULATIONS § 334.595 Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape...

33 CFR 334.595 - Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape Canaveral Air Force Station, FL...

Code of Federal Regulations, 2013 CFR

2013-07-01

...; 45th Space Wing, Cape Canaveral Air Force Station, FL; restricted area. 334.595 Section 334.595.... The regulations in this section shall be enforced by the Commander, 45th Space Wing, Patrick Air Force... AND RESTRICTED AREA REGULATIONS § 334.595 Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape...

33 CFR 334.595 - Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape Canaveral Air Force Station, FL...

Code of Federal Regulations, 2012 CFR

2012-07-01

...; 45th Space Wing, Cape Canaveral Air Force Station, FL; restricted area. 334.595 Section 334.595.... The regulations in this section shall be enforced by the Commander, 45th Space Wing, Patrick Air Force... AND RESTRICTED AREA REGULATIONS § 334.595 Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape...

33 CFR 334.595 - Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape Canaveral Air Force Station, FL...

Code of Federal Regulations, 2010 CFR

2010-07-01

...; 45th Space Wing, Cape Canaveral Air Force Station, FL; restricted area. 334.595 Section 334.595.... The regulations in this section shall be enforced by the Commander, 45th Space Wing, Patrick Air Force... AND RESTRICTED AREA REGULATIONS § 334.595 Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape...

33 CFR 334.595 - Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape Canaveral Air Force Station, FL...

Code of Federal Regulations, 2011 CFR

2011-07-01

...; 45th Space Wing, Cape Canaveral Air Force Station, FL; restricted area. 334.595 Section 334.595.... The regulations in this section shall be enforced by the Commander, 45th Space Wing, Patrick Air Force... AND RESTRICTED AREA REGULATIONS § 334.595 Atlantic Ocean off Cape Canaveral; 45th Space Wing, Cape...

Photocopy of drawing (original drawing of Theater886 Seats in possession ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing (original drawing of Theater-886 Seats in possession of MacDill Air Force Base, Civil Engineering, Tampa, Florida; 1947 architectural drawings by Department of the Army, Office of the Chief Engineers, Director of Military Construction Engineering Division, Washington, D.C.) FIRST FLOOR PLAN, ROOF PLAN, AND DETAILS - MacDill Air Force Base, Base Theater, 2420 Florida Keys Avenue, Tampa, Hillsborough County, FL

DETAIL, WINDOW ON THE NORTH FACADE, LOOKING SOUTH Eglin ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL, WINDOW ON THE NORTH FACADE, LOOKING SOUTH - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

Final Environmental Assessment For Demolishing Buildings 202 and 425 At Avon Park Air Force Range, Florida

DTIC Science & Technology

2005-08-01

ORGANIZATION NAME(S) AND ADDRESS(ES) Air Combat Command,Environmental Flight,Avon Park Air Force Range,FL,33825 8. PERFORMING ORGANIZATION REPORT...Oklahoma State University, 1973 Years of Experience: 21 Margaret Margosian Geographer Avon Park AFR, FL B.L.A., Landscape Architechture

76 FR 5142 - Procurement List; Additions and Deletion

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-28

... W6EC PEO STRI Orlando, FL. Service Type/Location: Base Supply Center, 2000 Wyoming Boulevard, Kirtland... Air Force, FA9401 377 CONS CC, Kirtland AFB, NM. Service Type/Location: Landscaping & Groundskeeping...

OBLIQUE VIEW OF WEST (FRONT) FACADE, LOOKING EAST/NORTHEAST Eglin ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OBLIQUE VIEW OF WEST (FRONT) FACADE, LOOKING EAST/NORTHEAST - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

Interior view of northeast unit master bedroom, looking into sleeping ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Interior view of northeast unit master bedroom, looking into sleeping porch, facing northeast - MacDill Air Force Base, Double Non-Commissioned Officers' Quarters, 7418 Hanger Loop Drive, Tampa, Hillsborough County, FL

INTERIOR OFFICES AT THE SOUTHERN END OF THE BUILDING, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

INTERIOR OFFICES AT THE SOUTHERN END OF THE BUILDING, LOOKING SOUTH - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

OBLIQUE VIEW OF WEST (FRONT) AND NORTH FACADES, LOOKING SOUTHEAST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OBLIQUE VIEW OF WEST (FRONT) AND NORTH FACADES, LOOKING SOUTHEAST - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

DETAILS, EAVES AND WINDOWS OF THE EAST (REAR) FACADE, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAILS, EAVES AND WINDOWS OF THE EAST (REAR) FACADE, LOOKING NORTH - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

VIEW OF TYPICAL WINDOW ON THE WEST (FRONT) FACADE, LOOKING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF TYPICAL WINDOW ON THE WEST (FRONT) FACADE, LOOKING EAST - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

Interior view of entry hall in Communication Center (now Break ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Interior view of entry hall in Communication Center (now Break Room and Storage Area), facing north - MacDill Air Force Base, Fire & Guard House, 2709 Florida Keys Avenue, Tampa, Hillsborough County, FL

Deciphering the groove binding modes of tau-fluvalinate and flumethrin with calf thymus DNA

NASA Astrophysics Data System (ADS)

Tao, Mo; Zhang, Guowen; Pan, Junhui; Xiong, Chunhong

2016-02-01

Tau-fluvalinate (TFL) and flumethrin (FL), widely used in agriculture and a class of synthetic pyrethroid pesticides with a similar structure, may cause a potential security risk. Herein, the modes of binding in vitro of TFL and FL with calf thymus DNA (ctDNA) were characterized by fluorescence, UV-vis absorption, circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy with the aid of viscosity measurements, melting analyses and molecular docking studies. The fluorescence titration indicated that both TFL and FL bound to ctDNA forming complexes through hydrogen bonding and van der Waals forces. The binding constants of TFL and FL with ctDNA were in the range of 104 L mol- 1, and FL exhibited a higher binding propensity than TFL. The iodide quenching effect, single/double-stranded DNA effects, and ctDNA melting and viscosity measurements demonstrated that the binding of both TFL and FL to ctDNA was groove mode. The FT-IR analyses suggested the A-T region of the minor groove of ctDNA as the preferential binding for TFL and FL, which was confirmed by the displacement assays with Hoechst 33258 probe, and the molecular docking visualized the specific binding. The changes in CD spectra indicated that both FL and TFL induced the perturbation on the base stacking and helicity of B-DNA, but the disturbance caused by FL was more obvious. Gel electrophoresis analyses indicated that both TFL and FL did not cause significant DNA cleavage. This study provides novel insights into the binding properties of TFL/FL with ctDNA and its toxic mechanisms.

Technology Transfer of the Air Quality Assessment Model.

DTIC Science & Technology

1984-02-01

i T I, _______ ENGINEERING & SERVICES LABORATORY AIR FORCE ENGINEERING & SERVICES CENTER TYNOALL AIR FORCE BASE. FLORIDA 32403 OTIC FILE CO84 03...30 015 NOTICE PLEASE DO NOT REQUEST COPIES OF THIS REPORT FRO(M HQ AFESC./RD ( ENGINEERING AND SERVICES LABORATORY). ADDITONAL COPIES MAY BE PURCHASED...report was prepared by the Air Force Engineering and Services Center, Engineering and Services Laboratory, (AFESC/ RDV) Tyndall AFB, FL. This report

View of Building No. 405 from Staff Circle, facing north ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of Building No. 405 from Staff Circle, facing north - MacDill Air Force Base, Bounded by City of Tampa North, Tampa Bay South, Old Tampa Bay West, & Hillsborough Bay East, Tampa, Hillsborough County, FL

Managing Fatigue in Long Duration Airlift Operations 1994

DTIC Science & Technology

2001-03-01

Air Force Research Laboratory Brooks Air Force Base, Texas, USA Abstract During September, 1994 the operational tempo for US Air Force C-5 transport...Nandina Terrace, Winter Springs, Fl 32708, USA Paper presented at the RTO HFM Workshop on "The Effect of Prolonged Military Activities in Man...drink. They were drinking lots of coffee and beer and most were eating a gastronomically difficult creation called a jumbo burger. We were given 24 hours

Improved Concrete Cutting and Excavation Capabilities for Crater Repair, Phase 1

DTIC Science & Technology

2014-04-01

manageable pieces, it is not recommended for the ADR process because of the requirement for additional supporting equipment - the air compressor ... Air Force Civil Engineer Center Tyndall Air Force Base, FL 32403-5319 ERDC/GSL TR-14-8 ii Abstract The US Army Engineer Research and...Development Center was tasked by the US Air Force Civil Engineer Center to improve the saw cutting and excavation production rates of crater repairs in thick

Electric Vehicle Modeling and Simulation.

DTIC Science & Technology

1983-08-01

RD-RI39 709 ELECTRIC VEHICLE MODELING RHD SIMULRTION(U) AIR FORCE lit INST OF TECH NRIGHT-PRTTERSON RFD OH SCHOOL OF ENGINEERING A R DEMISPELARE RUG...for Public Release Distribution Unlimited Fl School of Engineering Air Force Institute of Technology Wright-Patterson Air Force Base, Ohio Table of... Engineering , 49: 49-51 (27 August 1979). 36. Renner -Smith, S. "Battery-Saving Flywheel Gives Electric Car Freeway Zip," Popular Science, 215(10): 82-84

Field Demonstration of a Centrifugal Ultra High Pressure (UHP) P-19

DTIC Science & Technology

2010-03-01

States Air Force  Tyndall Air Force Base, FL 32403-5323 DISTRIBUTION A: Approved for public release; distribution unlimited. NOTICE AND SIGNATURE...PUBLICATION IN ACCORDANCE WITH ASSIGNED DISTRIBUTION STATEMENT. ___//SIGNATURE//______________________ ___//SIGNATURE//______________________ R...PROGRAM ELEMENT NUMBER 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 6. AUTHOR(S) 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8

Accountability,

DTIC Science & Technology

1987-05-01

8217i SIIOIl de ? le iave every- 0 h fl e .et r irjiit o-, base . Whv~y an-1l ow are weaccoutanlle to themonni-2 y Onie centraL reson is Lhat oo f (311 I51...FORCE MAXWELL AIR FORCE BASE , ALABAMA RELEASE; DSTR’BUTIONUNL MITED g’A 2 003 .,*,2 g oo Ii "A AIR WAR COLLEGE AIR UNIVERSITY hCCOUNTAB IL ITY by...TiHESIS ADVISOR: COLONEL KENNETH ii. WENKER MAXWELL AIR FORCE BASE , ALABAMA MAY 1987 LAcce.,:i F- INT!S rC,,A&I I DliC iAS [0 + r . - "- ’ ;...’C

DETAILS, ROOF VENTS AND WINDOWS, NORTHERN CORNER SECTION OF THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAILS, ROOF VENTS AND WINDOWS, NORTHERN CORNER SECTION OF THE EAST (REAR) FACADE, LOOKING NORTHWEST - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

DETAILS, ENTRY AND ROOF VENT, SOUTHERN SECTION OF THE WEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAILS, ENTRY AND ROOF VENT, SOUTHERN SECTION OF THE WEST (FRONT) FACADE, LOOKING NORTHEAST - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

OBLIQUE VIEW OF EAST (REAR) AND NORTH FACADES, WITH BUILDING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OBLIQUE VIEW OF EAST (REAR) AND NORTH FACADES, WITH BUILDING 792 VISIBLE IN BACKGROUND RIGHT, LOOKING WEST - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

Effect of a Metal Deactivator Fuel Additive on Fuel Deposition in Fuel Atomizers at High Temperature

DTIC Science & Technology

1992-08-01

ARMY NATICK RD&E CENTER DEVELOPMENT AND ENGINEERING CTR ATTN: SATNC-U ATUN : SATBE-F I NATICK MA 01760-5020 SATBE-FL 10 SATBE-BT 2 DIRECTOR SATBE-TQ 1...SFT (MR MAKRIS) I WASHINGTON DC 20330 SAALC/LDPE (MR ELLIOT) 1 KELLY AIR FORCE BASE TX 78241 CDR US AIR FORCE WRIGHT AERO LAB CDR ATUN : POSF (MR

Environmental Assessment of Installation Development at MacDill Air Force Base, Florida

DTIC Science & Technology

2007-01-01

effect. The essence of the Proposed Action is installation development, which includes demolition, renovation, repair, and new construction. This IDEA...Protection Attn: Jasmine Ruffington 3900 Commonwealth Blvd., MS 47 Tallahassee, FL 32399-3000 Florida State Clearinghouse Department of

INTERIOR, LARGE OPEN AREA AT THE NORTHERN END OF THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

INTERIOR, LARGE OPEN AREA AT THE NORTHERN END OF THE BUILDING, SHOWING CIRCULAR OPENINGS IN DIVIDING WALL, LOOKING NORTH - Eglin Air Force Base, Storehouse & Company Administration, Southeast of Flager Road, Nassau Lane, & southern edge of Weekly Bayou, Valparaiso, Okaloosa County, FL

Permeation Studies of CW Agents with Fabric Treated with 8-Hydroxyquinoline

DTIC Science & Technology

2017-12-01

Jeffery R. Owens AIR FORCE CIVIL ENGINEER CENTER AFCEC/CXAE, Tyndall AFB, FL 32403-5320 December 2017 Approved for public release: distribution...P.O. Box 68, Gunpowder, MD 21010-0068 Universal Technology Corp.; 139 Barnes Drive Suite 2 Tyndall AFB, FL 32404-5325 Air Force Civil Engineer ...moisture transport. The garment prevents exposure to outside chemical weapons agents (CWAs) by reacting or reducing permeation of the CWA. By

A biomechanical cadaveric study comparing superior capsule reconstruction using fascia lata allograft with human dermal allograft for irreparable rotator cuff tear.

PubMed

Mihata, Teruhisa; Bui, Christopher N H; Akeda, Masaki; Cavagnaro, Matthew A; Kuenzler, Michael; Peterson, Alexander B; McGarry, Michelle H; Itami, Yasuo; Limpisvasti, Orr; Neo, Masashi; Lee, Thay Q

2017-12-01

Biomechanical and clinical success of the superior capsule reconstruction (SCR) using fascia lata (FL) grafts has been reported. In the United States, human dermal (HD) allograft has been used successfully for SCRs; however, the biomechanical characteristics have not been reported. Eight cadaveric shoulders were tested in 5 conditions: (1) intact; (2) irreparable supraspinatus tear; (3) SCR using FL allograft with anterior and posterior suturing; (4) SCR using HD allograft with anterior and posterior suturing; and (5) SCR using HD allograft with posterior suturing. Rotational range of motion, superior translation, glenohumeral joint force, and subacromial contact were measured at 0°, 30°, and 60° of glenohumeral abduction in the scapular plane. Graft dimensions before and after testing were also recorded. Biomechanical parameters were compared using a repeated-measures analysis of variance with Tukey post hoc test, and graft dimensions were compared using a Student t-test (P < .05). Irreparable supraspinatus tear significantly increased superior translation, superior glenohumeral joint force, and subacromial contact pressure, which were completely restored with the SCR FL allografts. Both SCR HD allograft repairs partially restored superior translation and completely restored subacromial contact and superior glenohumeral joint force. The HD allografts significantly elongated by 15% during testing, whereas the FL allograft lengths were unchanged. Single-layered HD SCR allografts partially restored superior glenohumeral stability, whereas FL allograft SCR completely restored the superior glenohumeral stability. This may be due to the greater flexibility of the HD allograft, and the SCR procedure used was developed on the basis of FL grafts. Published by Elsevier Inc.

U.S. Special Operations Forces (SOF): Background and Issues for Congress

DTIC Science & Technology

2017-01-06

Defense. The Assistant Secretary of Defense for Special Operations and Low Intensity Conflict ( ASD /SOLIC), a member of the Office of the Secretary of...Defense for Policy (OSD-P), provides civilian oversight over USSOCOM activities. The current ASD /SOLIC is Mr. Michael Lumpkin, a former Navy SEAL...supports U.S. European Command; CCSA is the Army;  Special Operations Command Central (SOCCENT), MacDill Air Force Base, FL; supports U.S

23. "A CAPTIVE ATLAS MISSILE EXPLODED DURING THE TEST ON ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

23. "A CAPTIVE ATLAS MISSILE EXPLODED DURING THE TEST ON TEST STAND 1-A, 27 MARCH 1959, PUTTING THAT TEST STAND OUT-OF-COMMISSION. STAND WAS NOT REPAIRED FOR THE ATLAS PROGRAM BUT TRANSFERRED TO ROCKETDYNE AND MODIFIED FOR THE F-l ENGINE PROGRAM." - Edwards Air Force Base, Air Force Rocket Propulsion Laboratory, Test Stand 1-A, Test Area 1-120, north end of Jupiter Boulevard, Boron, Kern County, CA

Final Environmental Assessment for Camp Rudder Master Plan at Eglin Air Force Base, FL

DTIC Science & Technology

2005-06-07

warranting detailed analysis. Hazardous Materials The 6th RTB currently generates hazardous materials in the form of weapons cleaning products and...wastes. There would be no increase in the use of weapons cleaning products ; and therefore, this area does not require analysis. Additionally

Space Industry. Industry Study, Spring 2009

DTIC Science & Technology

2009-01-01

Space Flight Center, Cocoa Beach, FL Cape Canaveral Air Force Station, Cocoa Beach, FL Naval Ordnance Test Unit, Cocoa Beach, FL 50th Space Wing... America .” In 2009, as we celebrate the 40th anniversary of the fulfillment of that vision, it is appropriate to pause and reflect on how far we...value system, providing high-value services to both government and commercial consumers. The estimate of international and U.S. government consumption

B61 Joint Test Assembly (JTA) Weapons Systems Evaluation Program (WSEP) Eglin Air Force Base, FL Final Environmental Assessment

DTIC Science & Technology

2004-06-01

with TAs C-52A, C-52E, C-52N, and C-52W. It is used for air-to- ground munitions testing, countermeasures development and testing, and ground ...feet above ground level regardless of underlying land use . • Participating in “air shows” and fly-overs by U.S. Air Force aircraft at non-Air Force...Intermittent Intermittent 46 OSS Source : U.S. Government, 2001 Airway/Air Traffic Control The Warning Areas used by Eglin AFB are surrounded by

USMC’s Environmental Management Portal for EMS Implementation Service-wide Rollout and Future Plans

DTIC Science & Technology

2010-06-16

Si:e m Edit Page EM flortal HOMC Fnwnnm1~nt. Welcome to the E~l : xecJtive Order (:O) 13148, m~lemented at all aplicable Le9dership h Ewironmnt91...Buttalion Reserve Marine lntelliQence Support Mobile Forces Buttalion Reserve Marine Intelligence Support San Diego Forces Buttalion Reserve...washington, De CA LonQ Beach, Ca FL West Palm Beach, Fl LA New Orleans, La LA New Orleans, La AL Mobile , AI CA San Diego, Ca CA San Diego

A comparison of the performance of two advanced restraint systems in frontal impacts.

PubMed

Lopez-Valdes, F J; Juste, O; Pipkorn, B; Garcia-Muñoz, I; Sunnevång, C; Dahlgren, M; Alba, J J

2014-01-01

The goal of the study is to compare the kinematics and dynamics of the THOR dummy in a frontal impact under the action of 2 state-of-the-art restraint systems. Ten frontal sled tests were performed with THOR at 2 different impact speeds (35 and 9 km/h). Two advanced restraint systems were used: a pretensioned force-limiting belt (PT+FL) and a pretensioned belt incorporating an inflatable portion (PT+BB). Dummy measurements included upper and lower neck reactions, multipoint thoracic deflection, and rib deformation. Data were acquired at 10,000 Hz. Three-dimensional motion of relevant dummy landmarks was tracked at 1,000 Hz. RESULTS are reported in a local coordinate system moving with the test buck. Average forward displacement of the head was greater when the PT+FL belt was used (35 km/h: 376.3±16.1 mm [PT+BB] vs. 393.6±26.1 mm [PT+FL]; 9 km/h: 82.1±26.0 mm [PT+BB] vs. 98.8±0.2 mm [PT+FL]). The forward displacement of T1 was greater for the PT+FL belt at 35 km/h but smaller at 9 km/h. The forward motion of the pelvis was greater when the PT+BB was used, exhibiting a difference of 82 mm in the 9 km/h tests and 95.5 mm in the 35 km/h test. At 35 km/h, upper shoulder belt forces were similar (PT+FL: 4,756.8±116.6 N; PT+BB: 4,957.7±116.4 N). At 9 km/h, the PT+BB belt force was significantly greater than the PT+FL one. Lower neck flexion moments were higher for the PT+BB at 35 km/h but lower at 9 km/h (PT+FL: 34.2±3.5 Nm; PT+BB: 26.8±2.1 Nm). Maximum chest deflection occurred at the chest upper left region for both belts and regardless of the speed. The comparison of the performance of different restraints requires assessing occupant kinematics and dynamics from a global point of view. Even if the force acting on the chest is similar, kinematics can be substantially different. The 2 advanced belts compared here showed that while the PT+BB significantly reduced peak and resultant chest deflection, the resulting kinematics indicated an increased forward motion of the pelvis and a reduced rotation of the occupant's torso. Further research is needed to understand how these effects can influence the protection of real occupants in more realistic vehicle environments.

Environmental Assessment for Construction of Radial Arm Spill Gates MacDill Air Force Base, Florida

DTIC Science & Technology

2012-05-01

requires that Federal agencies identify and assess environmental health and safety risks that might disproportionately affect children. The Proposed...Action would not pose any adverse or disproportionate environmental health or safety risks to children living near the base. Safety precautions...Department of Envir. Protection 3900 Commonwealth Blvd, Mail Station 47 Tallahassee, FL 32399-3000 RE: Draft Environmental Assessment and Draft

Environmental Assessment for the Construction of the United States Air Force Technical Application Center (AFTAC) on Patrick Air Force Base, Florida

DTIC Science & Technology

2009-12-01

GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S...AND ADDRESS(ES) 45 Civil Engineer Squadron (45 CES/CEVP),1224 Jupiter Street,Patrick AFB,FL,32925 8. PERFORMING ORGANIZATION REPORT NUMBER 9...Quality Requirements Law or Rule Permit/Action(s) Requirement Agency or Organization AFI 32-7086, Chapter 4 Minimize loss and conduct recovery

United States Air Force Statistical Digest, Fiscal Year 1968. Twenty Third Edition

DTIC Science & Technology

1968-09-30

Tac1l1calMter - Total. f::Jiti. •• • • • •--;-:- • F-4Il •••• F-4.B•• F-l00fJ/F. F-IO$D/F. F- Uti • a:-W’aallteaopna1ssanae -1’otal • -40...Quarter Aircraft Quarter Hours On Hand uti 11- HOurs On Hand UU11- ANDS!RIIS, FlOlll1 During zaUon nown During zat10n ’. Quarter Rate Quarter Bate...8217MISSION :na.. DESIGN. AGE c . Uti - AVlm- ~ti-SERIES, ~ FLYING HOURS AGE FLYING HOURS WITHIN CoJllo. 11-. AIR- COJllo. 11-’ SortiesBASES u: bat za

Compliance Testing of Eielson Air Force Base Central Heating and Power Plant, Coal Fired Boiler Number 3, Eielson Air Force Base, Alaska

DTIC Science & Technology

1988-12-01

a flue gas grab sample for orsat analysis (measures oxygen, and carbon dioxide for stack gas molecular weight determination and emissions correction... molecular weight, was obtained during particulate sampling. Testing was initially started on boiler 2 at the maximum rated steam output of 120,000 lbs/hr...I, r*~td AN[D l FL B( R\\ ViQ( lPjN L!II(,t 1 (d) Open burning of put.rcible iear- i\\ % ~i~ emisions , ec.dirig c dn jc p. c ,f1 pre- cri :rig pair

Cape Canaveral Air Force Station, Launch Complex 39, Solid Rocket ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Cape Canaveral Air Force Station, Launch Complex 39, Solid Rocket Booster Disassembly & Refurbishment Complex, Thrust Vector Control Deservicing Facility, Hangar Road, Cape Canaveral, Brevard County, FL

Final Environmental Assessment: For Immediate Storm Surge Protection for Santa Rosa Island Facilities, Eglin Air Force Base, FL

DTIC Science & Technology

2006-06-01

ALTERNATIVES .............................................................. 2-1 2.1 Proposed Action ( Preferred Alternative...effects for all alternatives. This section also identifies the preferred alternative and discusses regulatory requirements and/or best management... Preferred Alternative) 06/23/06 Environmental Assessment Page 2-1 for Immediate Storm Surge Protection for Santa Rosa Island Facilities

COMBINED LABORATORY/FIELD STUDY ON THE USE OF NITRATE FOR IN SITU BIOREMEDIATION OF A FUEL-CONTAMINATED AQUIFER

EPA Science Inventory

A pilot demonstration project was conducted at Eglin Air Force Base, FL, to compare the extent of bioremediation of a fuel-contaminated aquifer using sprinkler application with and without nitrate addition on two adjacent 30 m x 30 m cells. Target compound groups included both B...

Laser Cooling for Heavy-Ion Fusion (HIF)

NASA Astrophysics Data System (ADS)

Ho, D. D.-M.; Brandon, S.; Lee, Y.

1997-05-01

A critical requirement for HIF is the ability to focus space-charge dominated beams onto a millimeter-size spot. However, chromatic aberration can result in a substantial fraction of the beam ions falling outside the spot radius. Because of the space-charge force, correcting the chromatic aberration using sextupoles is impractical. Success in laser cooling of low-current ion beams in storage rings leads us to explore the application of laser cooling to HIF. Basic scheme: After the beams have been accelerated to the desired energy by the recirculating induction linac, we let the beams coast around at constant energy. For efficient interaction between the laser and the beam ions, we use Ba+ beams. We use two lasers to pump the transitions in the Ba+ for generating the laser force FL. There is also an auxiliary force Fa, which is in the opposition direction of FL, provided by the induction cores. The momentum spread along the beam can be compressed by FL and Fa. We will present preliminary PIC simulations using the PIC code CONDOR. Potential difficulties caused by velocity space instabilities will be discussed.

The influence of memory and attention on the ear advantage in dichotic listening.

PubMed

D'Anselmo, Anita; Marzoli, Daniele; Brancucci, Alfredo

2016-12-01

The role of memory retention and attentional control on hemispheric asymmetry was investigated using a verbal dichotic listening paradigm, with the consonant-vowel syllables (/ba/,/da/,/ga/,/ka/,/pa/and/ta/), while manipulating the focus of attention and the time interval between stimulus and response. Attention was manipulated using three conditions: non-forced (NF), forced left (FL) and forced right (FR) attention. Memory involvement was varied using four delays (0, 1, 3 and 4 s) between stimulus presentation and response. Results showed a significant right ear advantage (REA) in the NF condition and an increased REA in the FR condition. A left ear advantage (LEA) was found in FL condition. The REA increased significantly in the NF attention condition at the 3-s compared to the 0-s delay and in the FR condition at the 1-s compared to the 0-s delay. No modulation of the left ear advantage was observed in the FL condition. These results are discussed in terms of an interaction between attentional processes and memory retention. Copyright © 2016 Elsevier B.V. All rights reserved.

Forecasting Cool Season Daily Peak Winds at Kennedy Space Center and Cape Canaveral Air Force Station

NASA Technical Reports Server (NTRS)

Barrett, Joe, III; Short, David; Roeder, William

2008-01-01

The expected peak wind speed for the day is an important element in the daily 24-Hour and Weekly Planning Forecasts issued by the 45th Weather Squadron (45 WS) for planning operations at Kennedy Space Center (KSC) and Cape Canaveral Air Force Station (CCAFS). The morning outlook for peak speeds also begins the warning decision process for gusts ^ 35 kt, ^ 50 kt, and ^ 60 kt from the surface to 300 ft. The 45 WS forecasters have indicated that peak wind speeds are a challenging parameter to forecast during the cool season (October-April). The 45 WS requested that the Applied Meteorology Unit (AMU) develop a tool to help them forecast the speed and timing of the daily peak and average wind, from the surface to 300 ft on KSC/CCAFS during the cool season. The tool must only use data available by 1200 UTC to support the issue time of the Planning Forecasts. Based on observations from the KSC/CCAFS wind tower network, surface observations from the Shuttle Landing Facility (SLF), and CCAFS upper-air soundings from the cool season months of October 2002 to February 2007, the AMU created multiple linear regression equations to predict the timing and speed of the daily peak wind speed, as well as the background average wind speed. Several possible predictors were evaluated, including persistence, the temperature inversion depth, strength, and wind speed at the top of the inversion, wind gust factor (ratio of peak wind speed to average wind speed), synoptic weather pattern, occurrence of precipitation at the SLF, and strongest wind in the lowest 3000 ft, 4000 ft, or 5000 ft. Six synoptic patterns were identified: 1) surface high near or over FL, 2) surface high north or east of FL, 3) surface high south or west of FL, 4) surface front approaching FL, 5) surface front across central FL, and 6) surface front across south FL. The following six predictors were selected: 1) inversion depth, 2) inversion strength, 3) wind gust factor, 4) synoptic weather pattern, 5) occurrence of precipitation at the SLF, and 6) strongest wind in the lowest 3000 ft. The forecast tool was developed as a graphical user interface with Microsoft Excel to help the forecaster enter the variables, and run the appropriate regression equations. Based on the forecaster's input and regression equations, a forecast of the day's peak and average wind is generated and displayed. The application also outputs the probability that the peak wind speed will be ^ 35 kt, 50 kt, and 60 kt.

Cape Canaveral Air Force Station, Launch Complex 39, The Solid ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Cape Canaveral Air Force Station, Launch Complex 39, The Solid Rocket Booster Assembly and Refurbishment Facility Manufacturing Building, Southeast corner of Schwartz Road and Contractors Road, Cape Canaveral, Brevard County, FL

Finding of No Significant Impact/Finding of No Practicable Alternative: Environmental Assessment of the Privatization of Military Family Housing at MacDill Air Force Base, Florida

DTIC Science & Technology

2007-10-09

specifically, the eastern portion of MacDill AFB, near the MFH parcels. The essence of the Proposed Action is redevelopment (i.e., demolition, road...Protection Attn: Jasmine Ruffington 3900 Commonwealth Blvd., MS 47 Tallahassee, FL 32399-3000 Florida State Clearinghouse Department of Environmental

View of Staff Officers' Quarters Area from Access Road. Building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of Staff Officers' Quarters Area from Access Road. Building No. 401 is to the left, Building No. 405 is to the right, and Building No. 403 is in center background. Facing northeast - MacDill Air Force Base, Bounded by City of Tampa North, Tampa Bay South, Old Tampa Bay West, & Hillsborough Bay East, Tampa, Hillsborough County, FL

View of Staff Officers' Quarters Area from Staff Circle. Building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of Staff Officers' Quarters Area from Staff Circle. Building No. 403 is to the right, Building No. 404 is to the left, and Building No. 405 is in far left in background. Facing west - MacDill Air Force Base, Bounded by City of Tampa North, Tampa Bay South, Old Tampa Bay West, & Hillsborough Bay East, Tampa, Hillsborough County, FL

Software Engineering Seminar (7th) 28 April - 2 May 1980, Elgin Air Force Base, Florida.

DTIC Science & Technology

1982-02-02

Wendell D. Thomas PMTC, Point Mugu, GA Jose Rodriguez, Jr. AFWTF, Rosevelt Roads, PR J. Paul Welch AD/KRA, Eglin AFB, FL Gwendolyn E. Hunt PNTC, Point... Eglin Air Force Base, Florida. It was agreed by the DR&GG membership, since many member ranges and centers were committing an increasing amount of...COMPLETION 1 OCTOBER 1981 ACTUAL COMPLETION 16 OCTOBER 1981 110 4 1%i ’I/) w 2M <U U) u < w DU C/- z U z W(0 U) 0 W 0 C-) ZU U 0 4J -i ix o z .... W W

Tailoring the charge carrier in few layers MoS2 field-effect transistors by Au metal adsorbate

NASA Astrophysics Data System (ADS)

Singh, Arun Kumar; Pandey, Rajiv K.; Prakash, Rajiv; Eom, Jonghwa

2018-04-01

It is an essential to tune the charge carrier concentrations in semiconductor in order to approach high-performance of the electronic and optoelectronic devices. Here, we report the effect of thin layer of gold (Au) metal on few layer (FL) molybdenum disulfide (MoS2) by atomic force microscopy (AFM), Raman spectroscopy and electrical charge transport measurements. The Raman spectra and charge transport measurements show that Au thin layer affect the electronic properties of the FL MoS2. After deposition of Au thin layer, the threshold voltages of FL MoS2 field-effect transistors (FETs) shift towards positive gate voltages, this reveal the p-doping in FL MoS2 nanosheets. The shift of peak frequencies of the Raman bands are also analyzed after the deposition of Au metal films of different thickness on FL MoS2 nanosheets. The surface morphology of Au metal on FL MoS2 is characterized by AFM and shows the smoother and denser film in comparison to Au metal on SiO2.

Muscle-specific deletion of exons 2 and 3 of the IL15RA gene in mice: effects on contractile properties of fast and slow muscles.

PubMed

O'Connell, Grant; Guo, Ge; Stricker, Janelle; Quinn, LeBris S; Ma, Averil; Pistilli, Emidio E

2015-02-15

Interleukin-15 (IL-15) is a putative myokine hypothesized to induce an oxidative skeletal muscle phenotype. The specific IL-15 receptor alpha subunit (IL-15Rα) has also been implicated in specifying this contractile phenotype. The purposes of this study were to determine the muscle-specific effects of IL-15Rα functional deficiency on skeletal muscle isometric contractile properties, fatigue characteristics, spontaneous cage activity, and circulating IL-15 levels in male and female mice. Muscle creatine kinase (MCK)-driven IL-15Rα knockout mice (mIl15ra(fl/fl)/Cre(+)) were generated using the Cre-loxP system. We tested the hypothesis that IL-15Rα functional deficiency in skeletal muscle would increase resistance to contraction-induced fatigue, cage activity, and circulating IL-15 levels. There was a significant effect of genotype on the fatigue curves obtained in extensor digitorum longus (EDL) muscles from female mIl15ra(fl/fl)/Cre(+) mice, such that force output was greater during the repeated contraction protocol compared with mIl15ra(fl/fl)/Cre(-) control mice. Muscles from female mIl15ra(fl/fl)/Cre(+) mice also had a twofold greater amount of the mitochondrial genome-specific COXII gene compared with muscles from mIl15ra(fl/fl)/Cre(-) control mice, indicating a greater mitochondrial density in these skeletal muscles. There was a significant effect of genotype on the twitch:tetanus ratio in EDL and soleus muscles from mIl15ra(fl/fl)/Cre(+) mice, such that the ratio was lower in these muscles compared with mIl15ra(fl/fl)/Cre(-) control mice, indicating a pro-oxidative shift in muscle phenotype. However, spontaneous cage activity was not different and IL-15 protein levels were lower in male and female mIl15ra(fl/fl)/Cre(+) mice compared with control. Collectively, these data support a direct effect of muscle IL-15Rα deficiency in altering contractile properties and fatigue characteristics in skeletal muscles.

THROW AWAY THE BOX: RETHINKING LOGISTICS INTEGRATION BETWEEN SPECIAL OPERATIONS AND GENERAL PURPOSE FORCES

DTIC Science & Technology

2015-02-13

Ft Carson CO; Logistics Officer, 10th Special Forces Group ( SFG ) Airborne (A) and Combined Joint Special Operations Task Force (CJSOTF) Arabian...ENDURING FREEDOM; Commander 7th SFG (A) Group Support Battalion, Eglin AFB, FL for two years and deployed to Afghanistan twice serving as the

Medical Surveillance Monthly Report (MSMR). Volume 15, Number 2, February/March 2008

DTIC Science & Technology

2008-03-01

Surveillance trends: Hyponatremia associated with heat stress and excessive water consumption: the impact of education and a new Army fl uid replacement... Environmental Medicine (USARIEM), Natick, Massachusetts, published new guidelines for fl uid replacement during military training in heat. Th e new...distributions of self-assessed health status as reported on deployment health assesment forms, U.S. Armed Forces, March 2007-February 2008 as “fair” or

Development of a Plantar Load Estimation Algorithm for Evaluation of Forefoot Load of Diabetic Patients during Daily Walks Using a Foot Motion Sensor

PubMed Central

Noguchi, Hiroshi; Sanada, Hiromi

2017-01-01

Forefoot load (FL) contributes to callus formation, which is one of the pathways to diabetic foot ulcers (DFU). In this study, we hypothesized that excessive FL, which cannot be detected by plantar load measurements within laboratory settings, occurs in daily walks. To demonstrate this, we created a FL estimation algorithm using foot motion data. Acceleration and angular velocity data were obtained from a motion sensor attached to each shoe of the subjects. The accuracy of the estimated FL was validated by correlation with the FL measured by force sensors on the metatarsal heads, which was assessed using the Pearson correlation coefficient. The mean of correlation coefficients of all the subjects was 0.63 at a level corridor, while it showed an intersubject difference at a slope and stairs. We conducted daily walk measurements in two diabetic patients, and additionally, we verified the safety of daily walk measurement using a wearable motion sensor attached to each shoe. We found that excessive FL occurred during their daily walks for approximately three hours in total, when any adverse event was not observed. This study indicated that FL evaluation method using wearable motion sensors was one of the promising ways to prevent DFUs. PMID:28840130

Development of a Plantar Load Estimation Algorithm for Evaluation of Forefoot Load of Diabetic Patients during Daily Walks Using a Foot Motion Sensor.

PubMed

Watanabe, Ayano; Noguchi, Hiroshi; Oe, Makoto; Sanada, Hiromi; Mori, Taketoshi

2017-01-01

Forefoot load (FL) contributes to callus formation, which is one of the pathways to diabetic foot ulcers (DFU). In this study, we hypothesized that excessive FL, which cannot be detected by plantar load measurements within laboratory settings, occurs in daily walks. To demonstrate this, we created a FL estimation algorithm using foot motion data. Acceleration and angular velocity data were obtained from a motion sensor attached to each shoe of the subjects. The accuracy of the estimated FL was validated by correlation with the FL measured by force sensors on the metatarsal heads, which was assessed using the Pearson correlation coefficient. The mean of correlation coefficients of all the subjects was 0.63 at a level corridor, while it showed an intersubject difference at a slope and stairs. We conducted daily walk measurements in two diabetic patients, and additionally, we verified the safety of daily walk measurement using a wearable motion sensor attached to each shoe. We found that excessive FL occurred during their daily walks for approximately three hours in total, when any adverse event was not observed. This study indicated that FL evaluation method using wearable motion sensors was one of the promising ways to prevent DFUs.

Use of a Commercially Available Flight Simulator during Aircrew Performance Testing.

DTIC Science & Technology

1991-11-01

Automiated Battery of Performance-based Tests, NAMRL 1354, Naval Aerospace Medical Research Laboratory, Pensacola, FL, 1990. 13. Human Performance...ability of an aircraft to remain airborne well beyond the limits of its human operator. This capacity for longer flights, coupled with a tendency for short...Measurement, Final Report, Air Force Human Resources Laboratory, Brooks AFB, TX, 1983. 5. Stein, E.S., Measurement of Pilot Performance: A Master Journeyman

75 FR 62810 - Twenty-Fourth Update of the Federal Agency Hazardous Waste Compliance Docket

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-13

... Alliance CCAFS Beach Road CCAFS FL 32920-9 Air Force 3010 19A Delta IV Program. 009 Transportation Security.... 171st Air Refueling Wing PAANG... 300 Tanker Rd....... Moon Township....... PA 15108 Air Force 3010 19A...

United States Air Force Summary, Sixth Edition

DTIC Science & Technology

1980-04-01

Schweiker, Richard S. (PA) Republicans Chiles, Lawton (FL) Johnston, J. Bennett, Jr. (IA) Huddleston, Walter D. (D") Burdick, Quentin N. (ND) Leahy...Stevens, Ted (AR) Schweiker, Richard s. (PA) Bellrron, Henry (OK) Republicans Chiles, Lawton (FL) Johnston, J. Bennett, Jr. (IA) Huddleston, ~7alter D...Janes R. (TN) Iaxalt, Paul (NV) • AF/ACBME/48no HOUSE APPROPRIATIONS COMMITTEE Darocrats REF 3 Whitten, Jamie L. (MS) Chai.rm3n Boland, Edward P. (MA

Installation Restoration Program. Phase II. Confirmation/Quantification. Stage 1 for Air Force Plant 6, Cobb County, Georgia. Volume 2.

DTIC Science & Technology

1985-08-09

FL UNLSSIFIED C R NEFF ET AL 99 AUG 95 F33615-84-D-4491 F/O 13/2 NLEmhANNE.mmmmhhhhl smhmhhhhmmhhls mhmmhmmhhhhhl...Occupational and Environmental Gainesville, FL 32602-3052 Health Laboratory I Brookq -kir ForrA R"Pn ’’y 1:;~ Sa. NAME OF FUNOINGiSPONSORING 8b. OFFICE...z ul N z~ I. * 4 11 z z 41 ( z a FL - pJ 0 :, w I.. 0 I z. z w \\, Cl) - u zzw JJ 4,..- 4.0 .J.. a A7 0 z I .4-i~ I _ _ __ _ _ C4.. % 0- .1 z IH~ID3M A

Development of EBM-CDSS (Evidence-Based Clinical Decision Support System) to AIG Prognostication in Terminally Ill Patients

DTIC Science & Technology

2016-03-01

Mbah, PhD2*, Ambuj Kumar, MD, MPH3, Kim Sehwan, PhD4*, Ronald Schonwetter, MD5* and Benjamin Djulbegovic, MD, PhD6 1Center for Evidence - Based Medicine , University...of South Florida, Tampa, FL 2USF, Tampa, FL 3University of South Florida, College of Medicine, Center for Evidence Based Medicine , Tampa...4HPC healthcare, Tampa, FL 5HPC Healthcare, Tampa, FL 6Center for Evidence - Based Medicine & Health Outcomes Research, University of South

Occupant restraint in the rear seat: ATD responses to standard and pre-tensioning, force-limiting belt restraints.

PubMed

Forman, Jason; Michaelson, Jarett; Kent, Richard; Kuppa, Shashi; Bostrom, Ola

2008-10-01

Recent studies have shown that restrained occupants over the age of 50 in frontal crashes have a higher risk of injury in the rear seat than in the front, and have hypothesized that the incorporation of technology such as belt pre-tensioning and force limiting preferentially in the front seat is at least partially responsible for this trend. This study investigates the potential benefits and trade-offs of seat belt pretensioners and force-limiters in the rear seat using a series of frontal impact sled tests at two speeds (48 km/h and 29 km/h DeltaV) with a buck representing the interior of the reat seat occupant compartment of a contemporary mid-sized sedan. Four different dummies were tested: the Hybrid III six year old (in a booster seat, H3 6YO), the Hybrid III 5(th) percentile female (H3 AF05), the Hybrid III 50(th) percentile male (H3 AM50), and the THOR-NT. The restraints consisted of either a standard three point belt, or a 3-point belt with a retractor pretensioner and a progressive force-limiter (FL+PT). Each test condition was repeated in triplicate. The FL+PT restraints (compared to the standard restraints) resulted in a significant (p < or = 0.05) decrease in peak internal chest deflection for each of the Hybrid III dummies at both test speeds (48 km/h: 29% decrease for H3 6YO, 38% decrease for H3 AF05, 30% decrease for H3 AM50), and for the THOR-NT at a DeltaV of 29 km/h. At 48 km/h, the FL+PT restraint qualitatively decreased the average peak internal chest deflection of the THOR-NT, however this decrease was not statistically significant (p=0.06). Furthermore, the FL+PT system allowed little or no increase in forward head excursion, and improved whole-body kinematics for all dummies by restricting pelvic excursion and slightly increasing torso pitch. The results suggest that the FL+PT system studied here may provide injury-reducing benefit to rear seat occupants in moderate to high severity frontal crashes, although more study is needed to evaluate these restraints in other crash scenarios.

Failure pressures after repairs of 2-cm × 2.5-cm rhinologic dural defects in a porcine ex vivo model.

PubMed

Lin, Ryan P; Weitzel, Erik Kent; Chen, Philip G; McMains, Kevin Christopher; Chang, Daniel R; Braxton, Ernest E; Majors, Jacob; Bunegin, Leon

2016-10-01

The objective of this study was to determine failure pressures of 6 rhinologic repair techniques of large skull base/dural defects in a controlled, ex vivo model. Failure pressures of 6 dural repairs in a porcine model were studied using a closed testing apparatus; 24-mm × 19-mm dural defects were created; 40-mm × 34-mm grafts composed of porcine Duragen (Integra), fascia lata, and Biodesign (Cook) were used either with or without Tisseel (Baxter International Inc.) to create 6 repairs: Duragen/no glue (D/NG), Duragen/Tisseel (D/T), fascia lata/no glue (FL/NG), fascia lata/Tisseel (FL/T), Biodesign/no glue (B/NG), and Biodesign/Tisseel (B/T). Saline was infused at 30 mL/hour, applying even force to the underside of the graft until repair failure. Five trials were performed per repair type for a total of 30 repairs. Mean failure pressures were as follows: D/NG 1.361 ± 0.169 cmH 2 O; D/T 9.127 ± 1.805 cmH 2 O; FL/NG 0.200 ± 0.109 cmH 2 O; FL/T 7.833 ± 2.657 cmH 2 O; B/NG 0.299 ± 0.109 cmH 2 O; and B/T 2.67 ± 0.619 cmH 2 O. There were statistically significant differences between glued (Tisseel) and non-glued repairs for each repair category (p < 0.05). All glued repairs performed better than non-glued repairs. Both D/T and FL/T repairs performed better than B/T repairs. No repair tolerated pressures throughout the full range of adult supine intracranial pressure. © 2016 ARS-AAOA, LLC.

United States Air Force Summary, Seventh Edition

DTIC Science & Technology

1981-01-01

Thomas F. (MO) Chiles, Lawton (FL) Democrats Andrews, Mark (~D) Abdnor, James (SD) Kasten, Robert W. Jr. (WI) D’Amato, Alfonse M. (~) Mattingly, Mack (r... Lawton (FL) Johnston, J. Bennett, Jr. (LA) Huddleston, Walter D. (KY) MillT AIY CON S T R U C II 0 N SUB COM MITT EE Republicans Laxalt, Paul (NV...Democrats REF 3 Whitten, Jamie L. (MS) Chairman Boland, Edward P. (MA) Na t cher, William H. (KY) Smith, Neal (IA) Addabbo, Joseph P. (NY) Long, Clarence D

The Relationship of Social Security Benefits and the Military Survivors Benefit Plan.

DTIC Science & Technology

1980-07-09

profound effect on the social security offset from the military survivors benefit plan (SBP). This study demonstrates the new social security computation...AD-ABO 088 AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB ON SCHOOL-ETC F/B 513 THE RELATIONSHIP OF SOCIAL SECURITY BENEFITS AND THE MILITARY S-ETC(U...JUL 80 W C LETZKUS. C R MARGENTHALER UNCLASSIFIED AFIT-LS-80-3 I fllflfflffl..fl fl ’IilllllNO] LEVE1L 0 9THE RELATIONSHIP OF SOCIAL SECURITY BENEFITS

Obama Kennedy Space Center Visit

NASA Image and Video Library

2010-04-14

President Barack Obama, left, exits of Air Force One with, from left, Representative US Representative Suzanne M. Kosmas (D - FL), U.S Senator Bill Nelson (D-FL), NASA Administrator Charles Bolden, and Apollo 11 Astronaut Buzz Aldrin after landing at the NASA Kennedy Space Center in Cape Canaveral, Fla. on Thursday, April 15, 2010. Obama visited Kennedy to deliver remarks on the bold new course the administration is charting to maintain U.S. leadership in human space flight. Photo Credit: (NASA/Bill Ingalls)

Installation Restoration Program Stage 3. McClellan Air Force Base Quality Assurance Project Plan

DTIC Science & Technology

1989-08-30

QUALITY ASSURANCE PROJECT PLAN TDTM FINAL ELECTE C\\ JAUG 3 119891 .. .. . .. 0.. ... PREPARD.FOR HEDURTR FL/E WRGTPATRO .. F... OHI.443 ........ 1N...P.E. Contract Program Manager \\ 0 RADMAN 0 TABLE OF CONTENTS Section Page 1.0 INTRODUCTION ................................................... 1 of 6...Section: 1.0 RADM Revision: 1 CO PO AIO NDate: 04/12/89 O Page 1 of 6 1.0 INTRODUCTION The Quality Assurance Project Plan (QAPP) presents in specific

Environmental Assessment for Ecosystem Restoration Masterplan Implementation at MacDill Air Force Base, Florida

DTIC Science & Technology

2013-05-01

PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) AMEC...Environment & Infrastructure, Inc,404 SW 140th Terrace,Newberry,FL,32669 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY... perform a coastal zone consistency evaluation. The State of Florida determined that the Proposed Action is consistent with the Florida CMP. FINDING

JTIDS Software and Test Engineering

DTIC Science & Technology

1994-08-01

AD-A284 134 Final Technical Report August 1994 / JTIDS SOFTWARE AND TEST ENGINEERING D TI’-C--• - Harris Corporation 5LP0 8 1994 Dennis Tebbe F W...PERFORMING ORGANIZATION Harris Corporation REPORT NUMBER P 0 Box 91000 N/A Melbourne FL 32902 a SPONOFNGIMONING AGENCY NAME($) AND ADORESS(ES) 10...Force Base, New York 94 900 186 This report has been reviewed by the Rome Laboratory Public Affairs Office (PA) and is releasable to the National

Military Assistance to Mexico: Use of Special Operations Forces

DTIC Science & Technology

2010-03-31

91 Donald Sparks, " Jackal Stone 2009; Special Opcmti011s Forces Multinational Training in Croatia", Tt/J of the Spear, USSCOM, Tampa FL, 92 Sparks...Data Analysis from 2001-2009" Justice in Mexico Project, Trans Border Institute, University of San Diego, January 2009. Sparks, Donald " Jackal Stone

Expansion of Countermine Lidar UAV-based System (CLUBS)

DTIC Science & Technology

2011-09-30

analysis with environmental conditions, namely; Ft. Lauderdale, FL, Looe Key, FL, Alpena , MI, and Buttermilk Bay, MA (Figure 2). We adopt a...estimated Dstat Looe Key, FL Ft. Lauderdale, FL Alpena , MI Buttermilk Bay, MA Figure 2. Regions of interests picked for the study to...Lauderdale, FL Alpena , MI Buttermilk Bay, MA Water properties Effective Fresnel reflectivity 0.002 0.0028 0.00144 0.0005 Diffuse

An inflatable belt system in the rear seat occupant environment: investigating feasibility and benefit in frontal impact sled tests with a 50th percentile male ATD

PubMed Central

Forman, Jason L.; Lopez-Valdes, Francisco J.; Dennis, Nate; Kent, Richard W.; Tanji, Hiromasa; Higuchi, Kazuo

2010-01-01

Frontal-impact airbag systems have the potential to provide a benefit to rear seat occupants by distributing restraining forces over the body in a manner not possible using belts alone. This study sought to investigate the effects of incorporating a belt-integrated airbag (“airbelt”) into a rear seat occupant restraint system. Frontal impact sled tests were performed with a Hybrid III 50th percentile male anthropomorphic test device (ATD) seated in the right-rear passenger position of a 2004 mid-sized sedan buck. Tests were performed at 48 km/h (20 g, 100 ms acceleration pulse) and 29 km/h (11 g, 100 ms). The restraints consisted of a 3-point belt system with a cylindrical airbag integrated into the upper portion of the shoulder belt. The airbag was tapered in shape, with a maximum diameter of 16 cm (at the shoulder) that decreased to 4 cm at the mid-chest. A 2.5 kN force-limiter was integrated into the shoulder-belt retractor, and a 2.3 kN pretensioner was present in the out-board anchor of the lap belt. Six ATD tests (three 48 km/h and three 29 km/h) were performed with the airbelt system. These were compared to previous frontal-impact, rear seat ATD tests with a standard (not-force-limited, not-pretensioned) 3-point belt system and a progressive force-limiting (peak 4.4 kN), pretensioning (FL+PT) 3-point belt system. In the 48 km/h tests, the airbelt resulted in significantly less (p<0.05, two-tailed Student’s t-test) posterior displacement of the sternum towards the spine (chest deflection) than both the standard and FL+PT belt systems (airbelt: average 13±1.1 mm standard deviation; standard belt: 33±2.3 mm; FL+PT belt: 23±2.6 mm). This was consistent with a significant reduction in the peak upper shoulder belt force (airbelt: 2.7±0.1 kN; standard belt: 8.7±0.3 kN; FL+PT belt: 4.4±0.1 kN), and was accompanied by a small increase in forward motion of the head (airbelt: 54±0.4 cm; standard belt: 45±1.3 cm; FL+PT belt: 47±1.1 cm) The airbelt system also significantly reduced the flexion moment in the lower neck (airbelt: 169±3.3 Nm; standard belt: 655±26 Nm; FL+PT belt: 308±19 Nm). Similar results were observed in the 29 km/h tests. These results suggest that this airbelt system may provide some benefit for adult rear seat occupants in frontal collisions, even in relatively low-speed impacts. Further study is needed to evaluate this type of restraint system for different size occupants (e.g., children), for out-of-position occupants, and with other occupant models (e.g., cadavers). PMID:21050596

MX Siting Investigation. Geotechnical Evaluation. Aggregate Resources Study, Lake Valley, Nevada.

DTIC Science & Technology

1981-02-27

KILOMETERS Mx SITING INVESTIGATION IGURE IPARTMENT OF TNt Ag1 FORCE - GMO 2 -_ONiO NATIONAL INC. FlU It FN-TR-37-f 5 2. Aerial and ground reconnaissance...fine, or crushed rock) and potential construction use ( con - crete and/or road base). TOM. FN-TR -37-f 6 2.0 STUDY APPROACH 2.1 EXISTING DATA Collection...2 the southwestern part of Lake Valley. This formation also pro - vides Class I crushed rock aggregate material in the southern White Rock Mountains

Hospitalizations Among Members of the Active Component, U.S. Armed Forces, 2011

DTIC Science & Technology

2012-04-01

immunity (240 - 279) 749 Skin and subcutaneous tissue (680 - 709) 1,978 Disorders of fl uid electrolyte and acid-base balance 242 32.3 Other cellulitis ...and abscess 1,442 72.9 Diabetes mellitus 225 30.0 Pilonidal cyst 164 8.3 Overweight, obesity and other hyperalimentation 60 8.0 Cellulitis and...280 - 289) 110 Skin and subcutaneous tissue (680 - 709) 204 Iron defi ciency anemias 39 35.5 Other cellulitis and abscess 116 56.9 Other and

Executive Report: JSOU (Joint Special Operations University) First Annual Symposium, 2-5 May 2006, Hurlburt Field, Florida

DTIC Science & Technology

2006-05-05

NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Joint Special Operations University,357 Tully Street...Alison Building,Hurlburt Field,FL,32544 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR...leadership. JSOU is a subordinate organization of the US Special Operations Command (USSOCOM), MacDill Air Force Base, Florida. The mission of the Joint

Final Environmental Assessment for the Okaloosa Regional Airport Expansion at Eglin Air Force Base, FL

DTIC Science & Technology

2006-06-01

construction Total daily trips are then applied to the following factors depending on the corresponding years. Year 2005 through 2009: VOCE ...016 * Trips NOxE = .015 * Trips PM10E = .0022 * Trips COE = .262 * Trips Year 2010 and beyond: VOCE = .012 * Trips NOxE = .013 * Trips PM10E...0022 * Trips COE = .262 * Trips To convert from pounds per day to tons per year: VOC (tons/yr) = VOCE * DPYII/2000 Nox (tons/yr) = NOxE

TET Offensive II Field Force Vietnam After Action Report 31 January - 18 February 1968

DTIC Science & Technology

1968-03-01

and the 5th VC Division. V During this same period of time there were no majur shifts in ARVN forces . However III Corps shifted three...8217-".•: ’ ’SSIFJED U.S. ARMY. VIETNAM. II FIELD FORCE . TET OFFENSIVE II FIELD FORCE VIETNAM AFTER ACTION REPORT, 31 JANUARY-18 FEB- RUARY 1968...H FIELD FORCE VIETNAM AFTER ACTION REPORT 31 January-18 February 1968 RECORD K0- ! FlSjl fi-.-A-,>-•: it tT*\\ : *si h s» -wP Mr-, £< St

Consolidation of Contingency Data and Its Use in Computer Graphics to Plan Bare Base Facility Construction at a Forward Operating Location.

DTIC Science & Technology

1983-09-01

34 o D Qa. : "lo °%S .. " ig.22. aveQuitComand P L %S dU a a Troubleshooting There are several potential problems that the user may encounter when...34 Air Force Civil Engineer, Vol. 3, No. 3 (August 1962 ), pp. 6-7. 40. Roberts, Captain Mike. "Automated Drafting and Design for the Base Civil Engineer... Eglin AFB FL, undated. 216 . - ,. ° - . .. . . .. r! 57. . Explosive Safety Standards. AFR 127-100. Chapter 5: Principles and Application of Explo- sive

DETAIL VIEW OF COMPUTER PANELS, ROOM 8A Cape Canaveral ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF COMPUTER PANELS, ROOM 8A - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

The DNA-recognition mode shared by archaeal feast/famine-regulatory proteins revealed by the DNA-binding specificities of TvFL3, FL10, FL11 and Ss-LrpB

PubMed Central

Yokoyama, Katsushi; Nogami, Hideki; Kabasawa, Mamiko; Ebihara, Sonomi; Shimowasa, Ai; Hashimoto, Keiko; Kawashima, Tsuyoshi; Ishijima, Sanae A.; Suzuki, Masashi

2009-01-01

The DNA-binding mode of archaeal feast/famine-regulatory proteins (FFRPs), i.e. paralogs of the Esherichia coli leucine-responsive regulatory protein (Lrp), was studied. Using the method of systematic evolution of ligands by exponential enrichment (SELEX), optimal DNA duplexes for interacting with TvFL3, FL10, FL11 and Ss-LrpB were identified as TACGA[AAT/ATT]TCGTA, GTTCGA[AAT/ATT]TCGAAC, CCGAAA[AAT/ATT]TTTCGG and TTGCAA[AAT/ATT]TTGCAA, respectively, all fitting into the form abcdeWWWedcba. Here W is A or T, and e.g. a and a are bases complementary to each other. Apparent equilibrium binding constants of the FFRPs and various DNA duplexes were determined, thereby confirming the DNA-binding specificities of the FFRPs. It is likely that these FFRPs recognize DNA in essentially the same way, since their DNA-binding specificities were all explained by the same pattern of relationship between amino-acid positions and base positions to form chemical interactions. As predicted from this relationship, when Gly36 of TvFL3 was replaced by Thr, the b base in the optimal DNA duplex changed from A to T, and, when Thr36 of FL10 was replaced by Ser, the b base changed from T to G/A. DNA-binding characteristics of other archaeal FFRPs, Ptr1, Ptr2, Ss-Lrp and LysM, are also consistent with the relationship. PMID:19468044

Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites.

PubMed

Zhu, Xinxin; Jin, Hui; Gao, Cuili; Gui, Rijun; Wang, Zonghua

2017-01-01

In this article, a facile aqueous synthesis of carbon dots (CDs) was developed by using natural kelp as a new carbon source. Through hydrothermal carbonization of kelp juice, fluorescent CDs were prepared and the CDs' surface was modified with polyethylenimine (PEI). The PEI-modified CDs were conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites. To exploit broad applications, the CDs-FITC composites were developed as fluorescent sensing or imaging platforms of pH and Cu 2+ . Analytical performances of the composites-based fluorescence (FL) sensors were evaluated, including visual FL imaging of pH in glass bottle, ratiometric FL sensing of pH in yogurt samples, visual FL latent fingerprint and leaf imaging detection of [Cu 2+ ], dual-signal FL sensing of [Cu 2+ ] in yogurt and human serum samples. Experimental results from ratiometric, visual, dual-signal FL sensing and imaging applications confirmed the high feasibility, accuracy, stabilization and simplicity of CDs-FITC composites-based FL sensors for the detection of pH and Cu 2+ ions in real samples. Copyright © 2016 Elsevier B.V. All rights reserved.

GPU Lossless Hyperspectral Data Compression System for Space Applications

NASA Technical Reports Server (NTRS)

Keymeulen, Didier; Aranki, Nazeeh; Hopson, Ben; Kiely, Aaron; Klimesh, Matthew; Benkrid, Khaled

2012-01-01

On-board lossless hyperspectral data compression reduces data volume in order to meet NASA and DoD limited downlink capabilities. At JPL, a novel, adaptive and predictive technique for lossless compression of hyperspectral data, named the Fast Lossless (FL) algorithm, was recently developed. This technique uses an adaptive filtering method and achieves state-of-the-art performance in both compression effectiveness and low complexity. Because of its outstanding performance and suitability for real-time onboard hardware implementation, the FL compressor is being formalized as the emerging CCSDS Standard for Lossless Multispectral & Hyperspectral image compression. The FL compressor is well-suited for parallel hardware implementation. A GPU hardware implementation was developed for FL targeting the current state-of-the-art GPUs from NVIDIA(Trademark). The GPU implementation on a NVIDIA(Trademark) GeForce(Trademark) GTX 580 achieves a throughput performance of 583.08 Mbits/sec (44.85 MSamples/sec) and an acceleration of at least 6 times a software implementation running on a 3.47 GHz single core Intel(Trademark) Xeon(Trademark) processor. This paper describes the design and implementation of the FL algorithm on the GPU. The massively parallel implementation will provide in the future a fast and practical real-time solution for airborne and space applications.

DEMONSTRATION OF IN SITU DEHALOGENATION OF DNAPL THROUGH INJECTION OF EMULSIFIED ZERO-VALIENT IRON AT LAUNCH COMPLEX 34 IN CAPE CANAVERAL AIR FORCE STATION, FLORIDA

EPA Science Inventory

The purpose of this project was to evaluate the technical and cost performance of emulsified zero-valent iron (EZVI) technology when applied to DNAPL contaminants in the saturated zone. This demonstration was conducted at Launch Complex 34, Cape Canaveral Air Force Station, FL, w...

75 FR 43398 - Amendment and Establishment of Restricted Areas and Other Special Use Airspace, Avon Park Air...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-26

... Use Airspace, Avon Park Air Force Range, FL AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... published in the Federal Register (75 FR 28752), Airspace Docket No. 06-ASO-18, to modify the special use airspace at the Avon Park Air Force Range in Florida. As a result of further review of the latitude...

Determination of Ammunition Training Rates for Marine Forces Study. Volume 2.

DTIC Science & Technology

1983-09-17

ARD-fl44 430 DETERMINATION OF AMMUNITION TRAINING RATES FOR MARINE i/n FORCES STUDY VOLUME 2(U) MARINE CORPS DEVELOPMENT AND EDUCATION COMMAND... STUDY - VOL II LIEUTENANT COLONEL R. J. YEOMAN C) DEPUTY CHIEF OF STAFF FOR DEVELOPMENTAL COORDINATION MY) DEVELOPMENT CENTER ’ MARINE CORPS...MARINE FORCES STUDY , DECISION S. PERFORMING ORG. REPORT NUMBER MEMORANDUM 7. AUTHOR(#) S. CONTR 4T9M GRANT NUMUER(s) M 00027- -G-0 060 LtCol R. J

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 3, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 3, FACING NORTH - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 3, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 3, FACING SOUTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 3, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 3, FACING EAST - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 4, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF OPERATIONS MANAGEMENT ROOM, FIRING ROOM NO. 4, FACING WEST - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF VIDEO MONITORS, FIRING ROOM NO. 2, FACING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF VIDEO MONITORS, FIRING ROOM NO. 2, FACING SOUTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF VIDEO MONITORS, FIRING ROOM NO. 3, FACING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF VIDEO MONITORS, FIRING ROOM NO. 3, FACING SOUTH - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Department of the Navy FY 1990/FY 1991 Biennial Budget Estimates. Military Construction and Family Housing Program FY 1990. Justification Data Submitted to Congress

DTIC Science & Technology

1989-01-01

Center, Panama City 314 Diver Training Building Addition 4,300 4 ,300 50 180 Subtotal 4,300 4,300 Navy Experimental Diving Unit 182 Panama City 347 ...Air Force 001 Security Training Center 4,500 4,500 100 345 Subtotal .4,500 4,500 TOTAL FOR TAS 28,220 28,220 Virginia Naval SecuritY Goui Activity 347 ...Building Addition 4,300 C NEDU Panama City, FL 347 Underwater Equipment Support Complex 2,900 C NPWC Pensacola, FL 109 Wastewater Transfer System 2,100 C

Installation Restoration Program. Phase 2. Confirmation/Quantification Stage 1 for Pease Air Force Base, New Hampshire. Volume 1.

DTIC Science & Technology

1987-08-01

01 0 4 In w el4 ’ n r fl f4 r, V 0 𔃾 1" * * * - ~ m ~c- I0 1-0- Q~ 3c 3c~ AdI-. * tp~dip Eu - 4j :: .( - * -U I’ -L - UJ a. Zw a Inc., ɘ L.3. 2-3...swale ap- proximately 100 feet south of Building 222 (Figure 3-7), the Jet Engine Test cell . With the exception of VOC, all param- eters detected at 15-B

Final Environmental Assessment for Construction of a Precision Measurement Equipment Laboratory Facility on Eglin Air Force Base, FL

DTIC Science & Technology

2006-10-01

daily trips are then applied to the following factors depending on the corresponding years. Year 2005 through 2009: VOCE = .016 * Trips NOxE...015 * Trips PM10E = .0022 * Trips COE = .262 * Trips Year 2010 and beyond: VOCE = .012 * Trips NOxE = .013 * Trips PM10E = .0022 * Trips COE...262 * Trips E = emissions To convert from pounds per day to tons per year: VOC (tons/yr) = VOCE * DPYII/2000 lbs/ton NOx (tons/yr) = NOxE * DPYII

LOUSMA, JACK R. - WATER SURVIVAL TRAINING - FL

NASA Image and Video Library

1978-07-31

S78-34037 (31 July 1978) --- Astronaut Jack R. Lousma, geared with a parachute, is pulled along behind a boat in Gulf waters at Homestead Air Force Base in Florida during a water survival training course attended by several NASA astronauts. The overall course is designed to familiarize astronauts with proper procedures to take in the event of ejection from an aircraft over water. Photo credit: NASA (NOTE: Since this photograph was made, astronaut Jack R. Lousma was named commander of STS-3, scheduled for launch in early spring of 1982.)

Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity.

PubMed

Weeks, Amy M; Coyle, Scott M; Jinek, Martin; Doudna, Jennifer A; Chang, Michelle C Y

2010-11-02

We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK is competent to protect its host from fluoroacetate toxicity in vivo and demonstrate a 10(6)-fold discrimination between fluoroacetyl-CoA (k(cat)/K(M) = 5 × 10⁷ M⁻¹ s⁻¹) and acetyl-CoA (k(cat)/K(M) = 30 M⁻¹ s⁻¹) based on a single fluorine substitution that originates from differences in both substrate reactivity and binding. We show that Thr 42, Glu 50, and His 76 are key catalytic residues and identify several factors that influence substrate selectivity. We propose that FlK minimizes interaction with the thioester carbonyl, leading to selection against acetyl-CoA binding that can be recovered in part by new C═O interactions in the T42S and T42C mutants. We hypothesize that the loss of these interactions is compensated by the entropic driving force for fluorinated substrate binding in a hydrophobic binding pocket created by a lid structure, containing Val 23, Leu 26, Phe 33, and Phe 36, that is not found in other structurally characterized members of this superfamily. We further suggest that water plays a critical role in fluorine specificity based on biochemical and structural studies focused on the unique Phe 36 "gate" residue, which functions to exclude water from the active site. Taken together, the findings from these studies offer molecular insights into organofluorine recognition and design of fluorine-specific enzymes.

Dietary Correlates of Primate Masticatory Muscle Fiber Architecture.

PubMed

Hartstone-Rose, Adam; Deutsch, Ashley R; Leischner, Carissa L; Pastor, Francisco

2018-02-01

Analyses of masticatory muscle architecture-specifically fascicle length (FL; a correlate of muscle stretch and contraction speed) and physiological cross-sectional area (PCSA; a correlate of force)-reveal soft-tissue dietary adaptations. For instance, consumers of large, soft foods are expected to have relatively long FL, while consumers of obdurate foods are expected to have relatively high PCSA. Unfortunately, only a few studies have analyzed these variables across large primate samples-an order of particular interest because it is our own. Previous studies found that, in strepsirrhines, force variables (PCSA and muscle masses; MM) scale with isometry or slight positive allometry, while the body size corrected FL residuals correlate with food sizes. However, a study of platyrrhines using different methods (in which the authors physically cut muscles between fascicles) found very different trends: negative allometry for both the stretch and force variables. Here, we apply the methods used in the strepsirrhine study (chemical dissection of fascicles to ensure full length measurements) to reevaluate these trends in platyrrhines and extend this research to include catarrhines. Our results conform to the previous strepsirrhine trends: there is no evidence of negative allometry in platyrrhines. Rather, in primates broadly and catarrhines specifically, MM and PCSA scale with isometry or positive allometry. When examining size-adjusted variables, it is clear that fascicle lengths (especially those of the temporalis muscle) correlate with diet: species that consume soft, larger, foods have longer masticatory fiber lengths which would allow them to open their jaws to wider gape angles. Anat Rec, 301:311-324, 2018. © 2018 Wiley Periodicals, Inc. © 2018 Wiley Periodicals, Inc.

Bite force estimation and the fiber architecture of felid masticatory muscles.

PubMed

Hartstone-Rose, Adam; Perry, Jonathan M G; Morrow, Caroline J

2012-08-01

Increasingly, analyses of craniodental dietary adaptations take into account mechanical properties of foods. However, masticatory muscle fiber architecture has been described for relatively few lineages, even though an understanding of the scaling of this anatomy can yield important information about adaptations for stretch and strength in the masticatory system. Data on the mandibular adductors of 28 specimens from nine species of felids representing nearly the entire body size range of the family allow us to evaluate the influence of body size and diet on the masticatory apparatus within this lineage. Masticatory muscle masses scale isometrically, tending toward positive allometry, with body mass and jaw length. This allometry becomes significant when the independent variable is a geometric mean of cranial variables. For all three body size proxies, the physiological cross-sectional area and predicted bite forces scale with significant positive allometry. Average fiber lengths (FL) tend toward negative allometry though with wide confidence intervals resulting from substantial scatter. We believe that these FL residuals are affected by dietary signals within the sample; though the mechanical properties of felid diets are relatively similar across species, the most durophagous species in our sample (the jaguar) appears to have relatively higher force production capabilities. The more notable dietary trend in our sample is the relationship between FL and relative prey size: felid species that predominantly consume relatively small prey have short masticatory muscle fibers, and species that regularly consume relatively large prey have relatively long fibers. This suggests an adaptive signal related to gape. Copyright © 2012 Wiley Periodicals, Inc.

GENERAL VIEW OF VEHICLE ACCESS PLATFORM CNORTH, HB3, FACING NORTHEAST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF VEHICLE ACCESS PLATFORM C-NORTH, HB-3, FACING NORTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF VEHICLE ACCESS PLATFORM ENORTH, HB3, FACING NORTH ...

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GENERAL VIEW OF VEHICLE ACCESS PLATFORM E-NORTH, HB-3, FACING NORTH - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF VEHICLE ACCESS PLATFORM ENORTH, HB3, FACING NORTHWEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF VEHICLE ACCESS PLATFORM E-NORTH, HB-3, FACING NORTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF VEHICLE ACCESS PLATFORM DNORTH, HB3, FACING NORTHWEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF VEHICLE ACCESS PLATFORM D-NORTH, HB-3, FACING NORTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF NORTH VEHICLE ACCESS PLATFORMS, HB3, FACING NORTHEAST ...

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GENERAL VIEW OF NORTH VEHICLE ACCESS PLATFORMS, HB-3, FACING NORTHEAST TOWARDS CEILING - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF VEHICLE ACCESS PLATFORM BNORTH, HB3, FACING NORTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF VEHICLE ACCESS PLATFORM B-NORTH, HB-3, FACING NORTH - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF VEHICLE ACCESS PLATFORM DNORTH, HB3, FACING NORTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF VEHICLE ACCESS PLATFORM D-NORTH, HB-3, FACING NORTH - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF NORTH VEHICLE ACCESS PLATFORMS, HB3, FACING NORTH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF NORTH VEHICLE ACCESS PLATFORMS, HB-3, FACING NORTH TOWARDS FLOOR - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF VEHICLE ACCESS PLATFORM BNORTH, HB3, FACING NORTHEAST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF VEHICLE ACCESS PLATFORM B-NORTH, HB-3, FACING NORTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF VEHICLE ACCESS PLATFORM CNORTH, HB3, FACING NORTHWEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF VEHICLE ACCESS PLATFORM C-NORTH, HB-3, FACING NORTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF THE POWER CONNECTIONS (FRONT) AND COMPUTER PANELS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF THE POWER CONNECTIONS (FRONT) AND COMPUTER PANELS (REAR), ROOM 8A - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF A VIDEO CAMERA POSITIONED ALONG THE PERIMETER ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF A VIDEO CAMERA POSITIONED ALONG THE PERIMETER OF THE MLP - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

EXTERIOR OF ALTITUDE CHAMBERS R (TO LEFT) AND L (TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

EXTERIOR OF ALTITUDE CHAMBERS R (TO LEFT) AND L (TO RIGHT), FACING NORTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

EXTERIOR OF ALTITUDE CHAMBERS R (TO LEFT) AND L (TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

EXTERIOR OF ALTITUDE CHAMBERS R (TO LEFT) AND L (TO RIGHT), FACING NORTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Dealing with time-varying recruitment and length in Hill-type muscle models.

PubMed

Hamouda, Ahmed; Kenney, Laurence; Howard, David

2016-10-03

Hill-type muscle models are often used in muscle simulation studies and also in the design and virtual prototyping of functional electrical stimulation systems. These models have to behave in a sufficiently realistic manner when recruitment level and contractile element (CE) length change continuously. For this reason, most previous models have used instantaneous CE length in the muscle׳s force vs. length (F-L) relationship, but thereby neglect the instability problem on the descending limb (i.e. region of negative slope) of the F-L relationship. Ideally CE length at initial recruitment should be used but this requires a multiple-motor-unit muscle model to properly account for different motor-units having different initial lengths when recruited. None of the multiple-motor-unit models reported in the literature have used initial CE length in the muscle׳s F-L relationship, thereby also neglecting the descending limb instability problem. To address the problem of muscle modelling for continuously varying recruitment and length, and hence different values of initial CE length for different motor-units, a new multiple-motor-unit muscle model is presented which considers the muscle to comprise 1000 individual Hill-type virtual motor-units, which determine the total isometric force. Other parts of the model (F-V relationship and passive elements) are not dependent on the initial CE length and, therefore, they are implemented for the muscle as a whole rather than for the individual motor-units. The results demonstrate the potential errors introduced by using a single-motor-unit model and also the instantaneous CE length in the F-L relationship, both of which are common in FES control studies. Copyright © 2016 Elsevier Ltd. All rights reserved.

Effect of tensile mean stress on fatigue behavior of single-crystal and directionally solidified superalloys

NASA Technical Reports Server (NTRS)

Kalluri, Sreeramesh; Mcgaw, Michael A.

1992-01-01

Two nickel base superalloys, single crystal PWA 1480 and directionally solidified MAR-M 246 + Hf, were studied in view of the potential usage of the former and usage of the latter as blade materials for the turbomachinery of the Space Shuttle main engine. The baseline zero mean stress (ZMS) fatigue life (FL) behavior of these superalloys was established, and then the effect of tensile mean stress (TMS) on their FL behavior was characterized. A stress range based FL prediction approach was used to characterize both the ZMS and TMS fatigue data. In the past, several researchers have developed methods to account for the detrimental effect of tensile mean stress on the FL for polycrystalline engineering alloys. These methods were applied to characterize the TMS fatigue data of single crystal PWA 1480 and directionally solidified MAR-M 246 + Hf and were found to be unsatisfactory. Therefore, a method of accounting for the TMS effect on FL, that is based on a technique proposed by Heidmann and Manson was developed to characterize the TMS fatigue data of these superalloys. Details of this method and its relationship to the conventionally used mean stress methods in FL prediction are discussed.

Studies of flerovium and element 115 homologs with macrocyclic extractants

NASA Astrophysics Data System (ADS)

Despotopulos, John Dustin

Study of the chemistry of the heaviest elements, Z ? 104, poses a unique challenge due to their low production cross-sections and short half-lives. Chemistry also must be studied on the one-atom-at-a-time scale, requiring automated, fast, and very efficient chemical schemes. Recent studies of the chemical behavior of copernicium (Cn, element 112) and flerovium (Fl, element 114) together with the discovery of isotopes of these elements with half-lives suitable for chemical studies have spurred a renewed interest in the development of rapid systems designed to study the chemical properties of elements with Z ≥ 114. This dissertation explores both extraction chromatography and solvent extraction as methods for development of a rapid chemical separation scheme for the homologs of flerovium (Pb, Sn, Hg) and element 115 (Bi, Sb), with the goal of developing a chemical scheme that, in the future, can be applied to on-line chemistry of both Fl and element 115. Macrocyclic extractants, specifically crown ethers and their derivatives, were chosen for these studies. Carrier-free radionuclides, used in these studies, of the homologs of Fl and element 115 were obtained by proton activation of high purity metal foils at the Lawrence Livermore National Laboratory (LLNL) Center for Accelerator Mass Spectrometry (CAMS): natIn(p,n)113Sn, natSn(p,n)124Sb, and Au(p,n)197m,gHg. The carrier-free activity was separated from the foils by novel separation schemes based on ion exchange and extraction chromatography techniques. Carrier-free Pb and Bi isotopes were obtained from development of a novel generator based on cation exchange chromatography using the 232U parent to generate 212Pb and 212Bi. Crown ethers show high selectivity for metal ions based on their size compared to the negatively charged cavity of the ether. Extraction by crown ethers occur based on electrostatic ion-dipole interactions between the negatively charged ring atoms (oxygen, sulfur, etc.) and the positively charged metal cations. Extraction chromatography resins produced by Eichrom Technologies, specifically the Pb resin based on di-t-byutlcyclohexano-18-crown-6, were chosen as a starting point for these studies. Simple chemical systems based solely on HCl matrices were explored to determine the extent of extraction for Pb, Sn and Hg on the resin. The kinetics and mechanism of extraction were also explored to determine suitability for a Fl chemical experiment. Systems based on KI/HCl and KI/HNO3 were explored for Bi and Sb. In both cases suitable separations, with high separation factors, were performed with vacuum flow columns containing the Pb-resin. Unfortunately the kinetics of uptake for Hg are far too slow on the traditional crown-ether to perform a Fl experiment and obtain whether or not Fl has true Hg-like character or not. However, the kinetics of Pb and Sn are more than sufficient for a Fl experiment to differentiate between Pb- or Sn-like character. To assess this kinetic issue a novel macrocyclic extractant based on sulfur donors was synthesized. Hexathia-18-crown-6, the sulfur analog of 18-crown-6, was synthesized based with by a template reaction using high dilution techniques. The replacement of oxygen ring atoms with sulfur should give the extractant a softer character, which should allow for far greater affinity toward soft metals such as Hg and Pb. From HCl matrices hexathia-18-crown-6 showed far greater kinetics and affinity for Hg than the Pb-resin; however, no affinity for Pb or Sn was seen. This presumably is due to the fact the charge density of sulfur crown ethers does not point to the center of the ring, and future synthesis of a substituted sulfur crown ether which forces the charge density to mimic that of the traditional crown ether should enable extraction of Pb and Sn to a greater extent than with the Pb-resin. Initial studies show promise for the separation of Bi and Sb from HCl matrices using hexathia-18-crown-6. Other macrocyclic extractants, including 2,2,2-cryptand, calix[6]arene and tetrathia-12-crown-4, were also investigated for comparison to the crown ethers. It was noted that these extractants are inferior compared to the crown and thiacrown ethers for extraction of Fl and element 115 homologs. A potential chemical system for Fl was established based on the Eichrom Pb resin, and insight to an improved system based on thiacrown ethers is presented.

VIEW OF FLIGHT CREW SYSTEMS, FLIGHT KITS FACILITY, ROOM NO. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF FLIGHT CREW SYSTEMS, FLIGHT KITS FACILITY, ROOM NO. 1N12, FACING NORTH - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

VIEW OF FLIGHT CREW SYSTEMS, FLIGHT KITS FACILITY, ROOM NO. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF FLIGHT CREW SYSTEMS, FLIGHT KITS FACILITY, ROOM NO. 1N12, FACING SOUTH - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF THE MAIN FLOOR LEVEL, PLATFORM DSOUTH, HB3, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF THE MAIN FLOOR LEVEL, PLATFORM D-SOUTH, HB-3, FACING NORTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF ELECTRONICS TEST AREA, FLIGHT KITS FACILITY, ROOM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF ELECTRONICS TEST AREA, FLIGHT KITS FACILITY, ROOM NO. 1N12, FACING WEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

GENERAL VIEW OF THE BRIDGE CRANE USED IN HB3 AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

GENERAL VIEW OF THE BRIDGE CRANE USED IN HB-3 AND HB-4, FACING SOUTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF BRIDGE CRANE USED TO LIFT DOMED LIDS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF BRIDGE CRANE USED TO LIFT DOMED LIDS OF THE ALTITUDE CHAMBERS, FACING SOUTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

VIEW OF COMPUTER/DATA COLLECTION AREA, SOUTH OF FIRING ROOM NO. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF COMPUTER/DATA COLLECTION AREA, SOUTH OF FIRING ROOM NO. 3, FACING SOUTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF VIDEO CAMERA, MAIN FLOOR LEVEL, PLATFORM ESOUTH, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF VIDEO CAMERA, MAIN FLOOR LEVEL, PLATFORM E-SOUTH, HB-3, FACING SOUTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

VIEW OF REMOTE MANIPULATOR SYSTEM LAB, ROOM NO. 1N4, FACING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF REMOTE MANIPULATOR SYSTEM LAB, ROOM NO. 1N4, FACING SOUTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF TESTING EQUIPMENT, REMOTE MANIPULATOR SYSTEM LAB, ROOM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF TESTING EQUIPMENT, REMOTE MANIPULATOR SYSTEM LAB, ROOM NO. 1N4, FACING SOUTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

VIEW OF REMOTE MANIPULATOR SYSTEM LAB, ROOM NO. 1N4, FACING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF REMOTE MANIPULATOR SYSTEM LAB, ROOM NO. 1N4, FACING NORTH - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Cost-effectiveness of quantitative fecal lactoferrin assay for diagnosis of symptomatic patients with ileal pouch-anal anastomosis.

PubMed

Parsi, Mansour A; Ellis, Jeffrey J; Lashner, Bret A

2008-08-01

To assess cost-effectiveness of fecal lactoferrin (FL) as the initial diagnostic approach to symptomatic patients with ileal pouch-anal anastomosis (IPAA). Four competing strategies [empiric metronidazole therapy (txMTZ), initial pouch endoscopy with biopsy (testBiop), initial FL assay followed by metronidazole therapy (testFL+MTZ), and initial FL assay followed by pouch endoscopy and biopsy (testFL+Biop)] were modeled in a decision tree. In the base-case, the average cost per patient was $241 for testFL+MTZ, $251 for txMTZ, $405 for testFL+Biop, and $431 for testBiop. The testBiop strategy had greater effectiveness compared with txMTZ but at an incremental cost of $158 per day. The txMTZ strategy was slightly more costly and minimally more effective than testFL+MTZ with an incremental cost effectiveness of just over $12 per day. However, the testFL+MTZ strategy was associated with a 31% absolute reduction in antibiotic exposure compared with the txMTZ strategy. Compared with empiric metronidazole therapy, FL before treatment with metronidazole is less costly with less exposure to antibiotics and less need for endoscopy, with only marginal decrease in effectiveness.

Environmental Assessment: Demolition of Munitions Storage Area Facilities at Ellsworth AFB, South Dakota

DTIC Science & Technology

2009-11-01

Eglin Parkway,Shalimar,FL,32579 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR...separately secured installation between 1950 and 1962 responsible for the storage, maintenance, and loading of atomic and thermonuclear weapons. The...portion of Ellsworth AFB and was operational between 1950 and 1962 (ACC 1997). It was operated by Air Force Materiel Command (AFMC), Armed Forces

Nondestructive Inspection (NDI) Facility Radiation Protection Survey for Homestead AFB, FL

DTIC Science & Technology

2012-10-31

worker radiation dosimetry records, Bioenvironmental Engineering’s occupational safety records, NDI’s operating procedures/instructions, radiation...Nondestructive Inspection Methods (2) Air Force Manual 48-125, Personnel Ionizing Radiation Dosimetry (3) Air Force Occupational Safety and Health Standard...radiography 3. TLDs properly stored (AFMAN 48-125; T.O. 33B-1-1, 6.8.5.4.4) 4. TLDs returned to storage rack at the end

77 FR 26229 - Meloy Channel, U.S. Coast Guard Base Miami Beach, FL; Restricted Area

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-03

....S. Coast Guard Base Miami Beach, FL; Restricted Area AGENCY: United States Army Corps of Engineers... the waters surrounding the U.S. Coast Guard Base Miami Beach, Florida (Base Miami Beach). Base Miami... corridors encompassing the waters immediately contiguous to Base Miami Beach. The amendment will also serve...

Transformation of follicular lymphoma to plasmablastic lymphoma with c-myc gene rearrangement.

PubMed

Ouansafi, Ihsane; He, Bing; Fraser, Cory; Nie, Kui; Mathew, Susan; Bhanji, Rumina; Hoda, Rana; Arabadjief, Melissa; Knowles, Daniel; Cerutti, Andrea; Orazi, Attilio; Tam, Wayne

2010-12-01

Follicular lymphoma (FL) is an indolent lymphoma that transforms to high-grade lymphoma, mostly diffuse large B-cell lymphoma, in about a third of patients. We present the first report of a case of FL that transformed to plasmablastic lymphoma (PBL). Clonal transformation of the FL to PBL was evidenced by identical IGH/BCL2 gene rearrangements and VDJ gene usage in rearranged IGH genes. IGH/ BCL2 translocation was retained in the PBL, which also acquired c-myc gene rearrangement. Genealogic analysis based on somatic hypermutation of the rearranged IGH genes of both FL and PBL suggests that transformation of the FL to PBL occurred most likely by divergent evolution from a common progenitor cell rather than direct evolution from the FL clone. Our study of this unusual case expands the histologic spectrum of FL transformation and increases our understanding of the pathogenetic mechanisms of transformation of indolent lymphomas to aggressive lymphomas.

Interactions of Histone Acetyltransferase p300 with the Nuclear Proteins Histone and HMGB1, As Revealed by Single Molecule Atomic Force Spectroscopy.

PubMed

Banerjee, S; Rakshit, T; Sett, S; Mukhopadhyay, R

2015-10-22

One of the important properties of the transcriptional coactivator p300 is histone acetyltransferase (HAT) activity that enables p300 to influence chromatin action via histone modulation. p300 can exert its HAT action upon the other nuclear proteins too--one notable example being the transcription-factor-like protein HMGB1, which functions also as a cytokine, and whose accumulation in the cytoplasm, as a response to tissue damage, is triggered by its acetylation. Hitherto, no information on the structure and stability of the complexes between full-length p300 (p300FL) (300 kDa) and the histone/HMGB1 proteins are available, probably due to the presence of unstructured regions within p300FL that makes it difficult to be crystallized. Herein, we have adopted the high-resolution atomic force microscopy (AFM) approach, which allows molecularly resolved three-dimensional contour mapping of a protein molecule of any size and structure. From the off-rate and activation barrier values, obtained using single molecule dynamic force spectroscopy, the biochemical proposition of preferential binding of p300FL to histone H3, compared to the octameric histone, can be validated. Importantly, from the energy landscape of the dissociation events, a model for the p300-histone and the p300-HMGB1 dynamic complexes that HAT forms, can be proposed. The lower unbinding forces of the complexes observed in acetylating conditions, compared to those observed in non-acetylating conditions, indicate that upon acetylation, p300 tends to weakly associate, probably as an outcome of charge alterations on the histone/HMGB1 surface and/or acetylation-induced conformational changes. To our knowledge, for the first time, a single molecule level treatment of the interactions of HAT, where the full-length protein is considered, is being reported.

DETAIL VIEW OF THE BRIDGE CRANE HOOKS USED IN HB3 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF THE BRIDGE CRANE HOOKS USED IN HB-3 AND HB-4, FACING SOUTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Graphene-based textured surface by pulsed laser deposition as a robust platform for surface enhanced Raman scattering applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tite, T.; Donnet, C.; Loir, A.-S.

We have developed a surface enhanced Raman scattering (SERS)-active substrate based on gold nanoparticles-decorated few-layer (fl) graphene grown by pulsed laser deposition. Diamond-Like Carbon film has been converted to fl-graphene after thermal annealing at low temperature. The formation of fl-graphene was confirmed by Raman spectroscopy, and surface morphology was highlighted by scanning electron microscopy. We found that textured fl-graphene film with nanoscale roughness was highly beneficial for SERS detection. Rhodamine 6G and p-aminothiophenol proposed as test molecules were detected with high sensitivity. The detection at low concentration of deltamethrin, an active molecule of a commercial pesticide was further demonstrated.

Intelligent control of a multi-degree-of freedom reaction compensating platform system using fuzzy logic

NASA Technical Reports Server (NTRS)

Choi, Benjamin B.; Lawrence, Charles; Lin, Yueh-Jaw

1994-01-01

This paper presents the development of a general-purpose fuzzy logic (FL) control methodology for isolating the external vibratory disturbances of space-based devices. According to the desired performance specifications, a full investigation regarding the development of an FL controller was done using different scenarios, such as variances of passive reaction-compensating components and external disturbance load. It was shown that the proposed FL controller is robust in that the FL-controlled system closely follows the prespecified ideal reference model. The comparative study also reveals that the FL-controlled system achieves significant improvement in reducing vibrations over passive systems.

77 FR 42652 - Meloy Channel, U.S. Coast Guard Base Miami Beach, FL; Restricted Area

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

....S. Coast Guard Base Miami Beach, FL; Restricted Area AGENCY: United States Army Corps of Engineers... Guard Base Miami Beach, Florida (Base Miami Beach). Base Miami Beach is composed of multiple U.S. Coast... contiguous to Base Miami Beach. The amendment will also serve to protect the general public from injury or...

Phosphate and acidosis act synergistically to depress peak power in rat muscle fibers.

PubMed

Nelson, Cassandra R; Debold, Edward P; Fitts, Robert H

2014-11-15

Skeletal muscle fatigue is characterized by the buildup of H(+) and inorganic phosphate (Pi), metabolites that are thought to cause fatigue by inhibiting muscle force, velocity, and power. While the individual effects of elevated H(+) or Pi have been well characterized, the effects of simultaneously elevating the ions, as occurs during fatigue in vivo, are still poorly understood. To address this, we exposed slow and fast rat skinned muscle fibers to fatiguing levels of H(+) (pH 6.2) and Pi (30 mM) and determined the effects on contractile properties. At 30°C, elevated Pi and low pH depressed maximal shortening velocity (Vmax) by 15% (4.23 to 3.58 fl/s) in slow and 31% (6.24 vs. 4.55 fl/s) in fast fibers, values similar to depressions from low pH alone. Maximal isometric force dropped by 36% in slow (148 to 94 kN/m(2)) and 46% in fast fibers (148 to 80 kN/m(2)), declines substantially larger than what either ion exerted individually. The strong effect on force combined with the significant effect on velocity caused peak power to decline by over 60% in both fiber types. Force-stiffness ratios significantly decreased with pH 6.2 + 30 mM Pi in both fiber types, suggesting these ions reduced force by decreasing the force per bridge and/or increasing the number of low-force bridges. The data indicate the collective effects of elevating H(+) and Pi on maximal isometric force and peak power are stronger than what either ion exerts individually and suggest the ions act synergistically to reduce muscle function during fatigue. Copyright © 2014 the American Physiological Society.

Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy*

PubMed Central

Woodall, Benjamin P.; Woodall, Meryl C.; Luongo, Timothy S.; Grisanti, Laurel A.; Tilley, Douglas G.; Elrod, John W.; Koch, Walter J.

2016-01-01

GRK2, a G protein-coupled receptor kinase, plays a critical role in cardiac physiology. Adrenergic receptors are the primary target for GRK2 activity in the heart; phosphorylation by GRK2 leads to desensitization of these receptors. As such, levels of GRK2 activity in the heart directly correlate with cardiac contractile function. Furthermore, increased expression of GRK2 after cardiac insult exacerbates injury and speeds progression to heart failure. Despite the importance of this kinase in both the physiology and pathophysiology of the heart, relatively little is known about the role of GRK2 in skeletal muscle function and disease. In this study we generated a novel skeletal muscle-specific GRK2 knock-out (KO) mouse (MLC-Cre:GRK2fl/fl) to gain a better understanding of the role of GRK2 in skeletal muscle physiology. In isolated muscle mechanics testing, GRK2 ablation caused a significant decrease in the specific force of contraction of the fast-twitch extensor digitorum longus muscle yet had no effect on the slow-twitch soleus muscle. Despite these effects in isolated muscle, exercise capacity was not altered in MLC-Cre:GRK2fl/fl mice compared with wild-type controls. Skeletal muscle hypertrophy stimulated by clenbuterol, a β2-adrenergic receptor (β2AR) agonist, was significantly enhanced in MLC-Cre:GRK2fl/fl mice; mechanistically, this seems to be due to increased clenbuterol-stimulated pro-hypertrophic Akt signaling in the GRK2 KO skeletal muscle. In summary, our study provides the first insights into the role of GRK2 in skeletal muscle physiology and points to a role for GRK2 as a modulator of contractile properties in skeletal muscle as well as β2AR-induced hypertrophy. PMID:27566547

Morphological and functional determinants of fluoxetine (Prozac)-induced pulmonary disease in an experimental model.

PubMed

Capelozzi, Marco A; Leick-Maldonado, Edna A; Parra, Edwin R; Martins, Mílton A; Tibério, Iolanda F L C; Capelozzi, Vera L

2007-05-14

Fluoxetine treatment effects were determined by evaluating respiratory mechanics (elastance/resistance) and exhaled nitric oxide, as well as mononuclear and polymorphonuclear cell recruitment into the lungs, in an experimental guinea pig model. Guinea pigs were divided into four groups: Fl (fluoxetine only, n=7); Fl+Sw (fluoxetine and forced swimming, n=7); Ns+Sw (normal saline and forced swimming, n=8); and Ns (normal saline only, n=8). Treated animals received oral fluoxetine (10 mg/(kg day)) for 30 consecutive days. On day 31, all animals were anesthetized and mechanically ventilated so that respiratory system elastance and resistance, as well exhaled nitric oxide, could be determined. The lungs were then excised en bloc for histological and immunohistochemical evaluation. Forced swimming induced bronchodilation in untreated animals and bronchoconstriction in fluoxetine-treated animals. Fluoxetine treatment was also associated with mononuclear infiltration (predominantly into alveolar walls) and neutrophil recruitment. In addition, levels of exhaled nitric oxide, an inflammatory marker, were higher in fluoxetine-treated animals. Swimming-induced stress also amplified mononuclear cell recruitment to the lungs. These results show that, in this experimental model, fluoxetine treatment reproduces the pathology of chronic interstitial pneumonia in humans.

Kinetics and mechanisms of 1,5-dihydroflavin reduction of carbonyl compounds and flavin oxidation of alcohols. III. Oxidation of benzoin by flavin and reduction of benzil by 1,5-dihydroflavin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bruice, T.C.; Taulane, J.P.

1976-11-24

The oxidation of benzoin by lumiflavin-3-acetic acid (Fl/sub ox/) to provide benzil and 1,5-dihydrolumiflavin-3-acetic acid (FlH/sub 2/) is a readily reversible reaction. It has been established that the mechanism involves general base ionization of benzoin carbon acid (..cap alpha..-ketol) to yield endiolate anion, followed by partitioning of the endiolate anion back to benzoin through general acid proton donation and to benzil by reaction with Fl/sub ox/. The reaction of endiolate anion with Fl/sub ox/ is not subject to acid or base catalysis. Evidence that ionization of benzoin precedes its oxidation by Fl/sub ox/ stems from the observation that the ratemore » attributed to the latter process possesses a constant equal to that for racemization of (+)-benzoin and O/sub 2/ oxidation of benzoin and that this rate constant is characterized by a primary deuterium kinetic isotope effect (k/sup benzoin//k/sup ..cap alpha..-/sup 2/H-benzoin/) of 7.24 +- 1.5. Reduction of benzil to benzoin by FlH/sub 2/ is pH and buffer insensitive below the pK/sub a/ of FlH/sub 2/. These results are consistent with either general acid catalyzed attack of benzoin carbanion at the 4a-position of Fl/sub ox/, followed by a specific base catalyzed collapse of adduct to diketone and dihydroflavin (Scheme III), or to the uncatalyzed reaction of carbanion (endiolate anion) with flavin to provide a semidione-flavin radical pair which then goes on to diketone and dihydroflavin in a non-acid-base catalyzed reaction (Scheme V). These mechanisms are discussed in terms of the kinetics of reaction of other carbanion species with flavin.« less

Environmental Assessment For the Proposed Construction of A Hazardous Materials Issue Facility and a Hazardous Wastes Storage Facility at Buckley Air Force Base, Colorado

DTIC Science & Technology

2005-04-01

July 2002, and expires 30 June 2007 (BAFB 2001). In July 2002, the CDPHE performed an inspection of...months during the winter, spring, and fall to 9.8 million gallons for the summer months, such as July . BAFB disposed of approximately 1,477 tons of...assistance, please contact Amy Pallante , our Section 106 Compliance Coordinator, at (303) 866-4678. Sincerely, 1 " . ~"l·v’\\. /,. , fl: ··[;uf. . UV’"\\.:\\... .. ~oc-Georgianna Contigugli v State Historic Preservation Officer

CORAL DISEASE & HEALTH CONSORTIUM; PARTNERS FOR PRESERVATION

EPA Science Inventory

Presented at EMAP Symposium 2001: Coastal Monitoring Through Partnerships, 24-27 April 2001, Pensacola Beach, FL.

The Coral Disease and Health Consortium (CDHC) was one recommendation to the U.S. Coral Reef Task Force (CRTF), to conserve the coral reef ecosystems of the U...

IST and METIL Overview

DTIC Science & Technology

2010-08-12

Research Disciplines  Partners/Sponsors  Markets Impacted  Select Projects  Future Goals and Needs 3 •Orlando, FL • 3rd largest University in U.S...ARMY AIR FORCE MARINE CORPS NAVY SAIC UNIVERSITY High School Simulation and Training Technology Center (STTC) INVIVO RESEARC H INC. Joint

The Influence of Physical Forcing on Bottom-water Dissolved Oxygen within the Caloosahatchee River Estuary, FL

EPA Science Inventory

Environmental Fluid Dynamic Code (EFDC), a numerical estuarine and coastal ocean circulation hydrodynamic model, was used to simulate the distribution of dissolved oxygen (DO), salinity, temperature, nutrients (nitrogen and phosphorus), and chlorophyll a in the Caloosahatchee Riv...

DETAIL VIEW OF A HANDHELD “BUMPER,” USED TO AID IN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF A HANDHELD “BUMPER,” USED TO AID IN POSITIONING THE ORBITER DURING STACKING PROCEDURES - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Enhanced Immune Response and Protective Effects of Nano-chitosan-based DNA Vaccine Encoding T Cell Epitopes of Esat-6 and FL against Mycobacterium Tuberculosis Infection

PubMed Central

Feng, Ganzhu; Jiang, Qingtao; Xia, Mei; Lu, Yanlai; Qiu, Wen; Zhao, Dan; Lu, Liwei; Peng, Guangyong; Wang, Yingwei

2013-01-01

Development of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nano-chitosan). The immunologic and protective efficacy of the nano-chitosan-based DNA vaccine (termed nano-Esat-6/3e-FL) was assessed in C57BL/6 mice after intramuscular prime vaccination with the plasmids DNA and nasal boost with the Esat-6/3e peptides. The results showed that the immunized mice remarkably elicited enhanced T cell responses and protection against M.tb H37Rv challenge. These findings indicate that the nano-chitosan can significantly elevate the immunologic and protective effects of the DNA vaccine, and the nano-Esat-6/3e-FL is a useful vaccine for preventing M.tb infection in mice. PMID:23637790

Player-Game Interaction: An Ecological Analysis of Foreign Language Gameplay Activities

ERIC Educational Resources Information Center

Ibrahim, Karim

2018-01-01

This article describes how the literature on game-based foreign language (FL) learning has demonstrated that player-game interactions have a strong potential for FL learning. However, little is known about the fine-grained dynamics of these interactions, or how they could facilitate FL learning. To address this gap, the researcher conducted a…

Young Foreign Language Learners' Interactions during Task-Based Paired Assessments

ERIC Educational Resources Information Center

Butler, Yuko Goto; Zeng, Wei

2014-01-01

Despite the popularity of task-based language teaching (TBLT) in foreign language (FL) education at elementary school, it remains unclear how young learners' FL abilities can best be evaluated with tasks. The present study seeks to understand developmental differences in interactions among elementary-school students during task-based language…

Predicting Rib Fracture Risk With Whole-Body Finite Element Models: Development and Preliminary Evaluation of a Probabilistic Analytical Framework

PubMed Central

Forman, Jason L.; Kent, Richard W.; Mroz, Krystoffer; Pipkorn, Bengt; Bostrom, Ola; Segui-Gomez, Maria

2012-01-01

This study sought to develop a strain-based probabilistic method to predict rib fracture risk with whole-body finite element (FE) models, and to describe a method to combine the results with collision exposure information to predict injury risk and potential intervention effectiveness in the field. An age-adjusted ultimate strain distribution was used to estimate local rib fracture probabilities within an FE model. These local probabilities were combined to predict injury risk and severity within the whole ribcage. The ultimate strain distribution was developed from a literature dataset of 133 tests. Frontal collision simulations were performed with the THUMS (Total HUman Model for Safety) model with four levels of delta-V and two restraints: a standard 3-point belt and a progressive 3.5–7 kN force-limited, pretensioned (FL+PT) belt. The results of three simulations (29 km/h standard, 48 km/h standard, and 48 km/h FL+PT) were compared to matched cadaver sled tests. The numbers of fractures predicted for the comparison cases were consistent with those observed experimentally. Combining these results with field exposure informantion (ΔV, NASS-CDS 1992–2002) suggests a 8.9% probability of incurring AIS3+ rib fractures for a 60 year-old restrained by a standard belt in a tow-away frontal collision with this restraint, vehicle, and occupant configuration, compared to 4.6% for the FL+PT belt. This is the first study to describe a probabilistic framework to predict rib fracture risk based on strains observed in human-body FE models. Using this analytical framework, future efforts may incorporate additional subject or collision factors for multi-variable probabilistic injury prediction. PMID:23169122

Robotic application of a dynamic resultant force vector using real-time load-control: simulation of an ideal follower load on Cadaveric L4-L5 segments.

PubMed

Bennett, Charles R; Kelly, Brian P

2013-08-09

Standard in-vitro spine testing methods have focused on application of isolated and/or constant load components while the in-vivo spine is subject to multiple components that can be resolved into resultant dynamic load vectors. To advance towards more in-vivo like simulations the objective of the current study was to develop a methodology to apply robotically-controlled, non-zero, real-time dynamic resultant forces during flexion-extension on human lumbar motion segment units (MSU) with initial application towards simulation of an ideal follower load (FL) force vector. A proportional-integral-derivative (PID) controller with custom algorithms coordinated the motion of a Cartesian serial manipulator comprised of six axes each capable of position- or load-control. Six lumbar MSUs (L4-L5) were tested with continuously increasing sagittal plane bending to 8 Nm while force components were dynamically programmed to deliver a resultant 400 N FL that remained normal to the moving midline of the intervertebral disc. Mean absolute load-control tracking errors between commanded and experimental loads were computed. Global spinal ranges of motion and sagittal plane inter-body translations were compared to previously published values for non-robotic applications. Mean TEs for zero-commanded force and moment axes were 0.7 ± 0.4N and 0.03 ± 0.02 Nm, respectively. For non-zero force axes mean TEs were 0.8 ± 0.8 N, 1.3 ± 1.6 Nm, and 1.3 ± 1.6N for Fx, Fz, and the resolved ideal follower load vector FL(R), respectively. Mean extension and flexion ranges of motion were 2.6° ± 1.2° and 5.0° ± 1.7°, respectively. Relative vertebral body translations and rotations were very comparable to data collected with non-robotic systems in the literature. The robotically coordinated Cartesian load controlled testing system demonstrated robust real-time load-control that permitted application of a real-time dynamic non-zero load vector during flexion-extension. For single MSU investigations the methodology has potential to overcome conventional follower load limitations, most notably via application outside the sagittal plane. This methodology holds promise for future work aimed at reducing the gap between current in-vitro testing and in-vivo circumstances. Copyright © 2013 Elsevier Ltd. All rights reserved.

Design and force analysis of end-effector for plug seedling transplanter.

PubMed

Jiang, Zhuohua; Hu, Yang; Jiang, Huanyu; Tong, Junhua

2017-01-01

Automatic transplanters have been very important in greenhouses since the popularization of seedling nurseries. End-effector development is a key technology for transplanting plug seedlings. Most existing end-effectors have problems with holding root plugs or releasing plugs. An efficient end-effector driven by a linear pneumatic cylinder was designed in this study, which could hold root plugs firmly and release plugs easily. This end-effector with four needles could clamp the plug simultaneously while the needles penetrate into the substrate. The depth and verticality of the needles could be adjusted conveniently for different seedling trays. The effectiveness of this end-effector was tested by a combinational trial examining three seedling nursery factors (the moisture content of the substrate, substrate bulk density and the volume proportion of substrate ingredients). Results showed that the total transplanting success rate for the end-effector was 100%, and the root plug harm rate was below 17%. A force measure system with tension and pressure transducers was installed on the designed end-effector. The adhesive force FL between the root plug and the cell of seedling trays and the extrusion force FK on the root plug were measured and analyzed. The results showed that all three variable factors and their interactions had significant effects on the extrusion force. Each factor had a significant effect on adhesive force. Additionally, it was found that the end-effector did not perform very well when the value of FK/FL was beyond the range of 5.99~8.67. This could provide a scientific basis for end-effector application in transplanting.

Design and force analysis of end-effector for plug seedling transplanter

PubMed Central

Hu, Yang; Jiang, Huanyu; Tong, Junhua

2017-01-01

Automatic transplanters have been very important in greenhouses since the popularization of seedling nurseries. End-effector development is a key technology for transplanting plug seedlings. Most existing end-effectors have problems with holding root plugs or releasing plugs. An efficient end-effector driven by a linear pneumatic cylinder was designed in this study, which could hold root plugs firmly and release plugs easily. This end-effector with four needles could clamp the plug simultaneously while the needles penetrate into the substrate. The depth and verticality of the needles could be adjusted conveniently for different seedling trays. The effectiveness of this end-effector was tested by a combinational trial examining three seedling nursery factors (the moisture content of the substrate, substrate bulk density and the volume proportion of substrate ingredients). Results showed that the total transplanting success rate for the end-effector was 100%, and the root plug harm rate was below 17%. A force measure system with tension and pressure transducers was installed on the designed end-effector. The adhesive force FL between the root plug and the cell of seedling trays and the extrusion force FK on the root plug were measured and analyzed. The results showed that all three variable factors and their interactions had significant effects on the extrusion force. Each factor had a significant effect on adhesive force. Additionally, it was found that the end-effector did not perform very well when the value of FK/FL was beyond the range of 5.99~8.67. This could provide a scientific basis for end-effector application in transplanting. PMID:28678858

VIEW OF HB1 (VAB HIGH BAY) WITH MOBILE LAUNCHER PLATFORM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF HB-1 (VAB HIGH BAY) WITH MOBILE LAUNCHER PLATFORM (VEHICLE ACCESS PLATFORMS ARE VISIBLE IN THE CENTER), FACING WEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

EZVI Injection Field Test Leads to Pilot-Scale Application

EPA Science Inventory

Testing and monitoring of emulsified zero-valent ironTM (EZVI) injections was conducted at Cape Canaveral Air Force Station’s Launch Complex 34, FL, in 2002 to 2005 to evaluate the technology’s efficacy in enhancing in situ dehalogenation of dense nonaqueous-phase liquid (DNAPL) ...

DETAIL VIEW OF WINCH USED TO RAISE AND LOWER AP ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF WINCH USED TO RAISE AND LOWER AP 93W, ROOF LEVEL, PLATFORM D-SOUTH, HB-3, FACING WEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL VIEW OF WINCH USED TO RAISE AND LOWER HINGED ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF WINCH USED TO RAISE AND LOWER HINGED SECTION OF THIRD FLOOR LEVEL, PLATFORM D-SOUTH, HB-3, FACING WEST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Final Environmental Assessment for Constructing and Operating Remoted Target Systems at Avon Park Air Force Range, Florida

DTIC Science & Technology

2003-12-01

NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Air Combat Command,Environmental Flight,Avon Park Air Force Range...FL,33825 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM(S) 11...Oscar Range are either Spodosols or Alfisols. Spodosols soils are characterized by a subsurface zone called a spodic ( organic ) horizon layer, whereas

Air Force Civil Engineer, Volume 15, Number 4, 2007, 2007 Almanac

DTIC Science & Technology

2007-01-01

Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour...subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. 1 . REPORT DATE...PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Air Force Civil Engineer (AFCESA/PCT),139 Barnes Drive, Suite 1 ,Tyndall AFB,FL,32403-5319 8

Distinct Molecular Signature of Murine Fetal Liver and Adult Hematopoietic Stem Cells Identify Novel Regulators of Hematopoietic Stem Cell Function

PubMed Central

Manesia, Javed K.; Franch, Monica; Tabas-Madrid, Daniel; Nogales-Cadenas, Ruben; Vanwelden, Thomas; Van Den Bosch, Elisa; Xu, Zhuofei; Pascual-Montano, Alberto; Khurana, Satish; Verfaillie, Catherine M.

2018-01-01

During ontogeny, fetal liver (FL) acts as a major site for hematopoietic stem cell (HSC) maturation and expansion, whereas HSCs in the adult bone marrow (ABM) are largely quiescent. HSCs in the FL possess faster repopulation capacity as compared with ABM HSCs. However, the molecular mechanism regulating the greater self-renewal potential of FL HSCs has not yet extensively been assessed. Recently, we published RNA sequencing-based gene expression analysis on FL HSCs from 14.5-day mouse embryo (E14.5) in comparison to the ABM HSCs. We reanalyzed these data to identify key transcriptional regulators that play important roles in the expansion of HSCs during development. The comparison of FL E14.5 with ABM HSCs identified more than 1,400 differentially expressed genes. More than 200 genes were shortlisted based on the gene ontology (GO) annotation term “transcription.” By morpholino-based knockdown studies in zebrafish, we assessed the function of 18 of these regulators, previously not associated with HSC proliferation. Our studies identified a previously unknown role for tdg, uhrf1, uchl5, and ncoa1 in the emergence of definitive hematopoiesis in zebrafish. In conclusion, we demonstrate that identification of genes involved in transcriptional regulation differentially expressed between expanding FL HSCs and quiescent ABM HSCs, uncovers novel regulators of HSC function. PMID:27958775

DETAIL VIEW OF SRB/ET ACCESS PANELS, THROUGH VEHICLE ACCESS PLATFORMS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL VIEW OF SRB/ET ACCESS PANELS, THROUGH VEHICLE ACCESS PLATFORMS E-NORTH, E-SOUTH, B-NORTH, AND B-SOUTH, HB-3, FACING NORTH - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

DETAIL OF THE LIQUID HYDROGEN AND LIQUID OXYGEN VENT VALVES, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

DETAIL OF THE LIQUID HYDROGEN AND LIQUID OXYGEN VENT VALVES, SIXTH LEVEL OF THE EXTERNAL TANK CHECK-OUT CELLS, HB-2, FACING NORTHEAST - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

78 FR 17085 - Amendment of Multiple Restricted Areas; Eglin AFB, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-20

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 73 [Docket No. FAA-2013... date: 0901 UTC, May 2, 2013. FOR FURTHER INFORMATION CONTACT: Paul Gallant, Airspace Policy and ATC... [Amended] By removing the words ``Using agency. U.S. Air Force, Commander, Air Armament Center, Eglin AFB...

Predicting subcontractor performance using web-based Evolutionary Fuzzy Neural Networks.

PubMed

Ko, Chien-Ho

2013-01-01

Subcontractor performance directly affects project success. The use of inappropriate subcontractors may result in individual work delays, cost overruns, and quality defects throughout the project. This study develops web-based Evolutionary Fuzzy Neural Networks (EFNNs) to predict subcontractor performance. EFNNs are a fusion of Genetic Algorithms (GAs), Fuzzy Logic (FL), and Neural Networks (NNs). FL is primarily used to mimic high level of decision-making processes and deal with uncertainty in the construction industry. NNs are used to identify the association between previous performance and future status when predicting subcontractor performance. GAs are optimizing parameters required in FL and NNs. EFNNs encode FL and NNs using floating numbers to shorten the length of a string. A multi-cut-point crossover operator is used to explore the parameter and retain solution legality. Finally, the applicability of the proposed EFNNs is validated using real subcontractors. The EFNNs are evolved using 22 historical patterns and tested using 12 unseen cases. Application results show that the proposed EFNNs surpass FL and NNs in predicting subcontractor performance. The proposed approach improves prediction accuracy and reduces the effort required to predict subcontractor performance, providing field operators with web-based remote access to a reliable, scientific prediction mechanism.

Predicting Subcontractor Performance Using Web-Based Evolutionary Fuzzy Neural Networks

PubMed Central

2013-01-01

Subcontractor performance directly affects project success. The use of inappropriate subcontractors may result in individual work delays, cost overruns, and quality defects throughout the project. This study develops web-based Evolutionary Fuzzy Neural Networks (EFNNs) to predict subcontractor performance. EFNNs are a fusion of Genetic Algorithms (GAs), Fuzzy Logic (FL), and Neural Networks (NNs). FL is primarily used to mimic high level of decision-making processes and deal with uncertainty in the construction industry. NNs are used to identify the association between previous performance and future status when predicting subcontractor performance. GAs are optimizing parameters required in FL and NNs. EFNNs encode FL and NNs using floating numbers to shorten the length of a string. A multi-cut-point crossover operator is used to explore the parameter and retain solution legality. Finally, the applicability of the proposed EFNNs is validated using real subcontractors. The EFNNs are evolved using 22 historical patterns and tested using 12 unseen cases. Application results show that the proposed EFNNs surpass FL and NNs in predicting subcontractor performance. The proposed approach improves prediction accuracy and reduces the effort required to predict subcontractor performance, providing field operators with web-based remote access to a reliable, scientific prediction mechanism. PMID:23864830

Listening to Students: Modification of a Reading Program Based on the Sources of Foreign Language Reading Anxiety

ERIC Educational Resources Information Center

Aydin, Belgin; Kuru Gonen, Ipek

2012-01-01

This paper is concerned with the modifications implemented in a second year foreign language (FL) reading program with respect to the problems students experience while reading in FL. This research draws on the sources of FL reading anxiety identified in the first year reading program with a motivation to re-design the second year program to help…

[Prevalence of extrinsic allergic alveolitis in cattle breeders from the province of Reggio Emilia].

PubMed

Ferri, F; Ruggieri, Maria Paola; Guidetti, G; Azzarone, G; Giammartini, Pasquina; Capanni, S; Mantovani, P; Bertani, Marisa

2003-01-01

Several new cases of Extrinsic Allergic Alveolitis or Farmer's Lung (FL) in farm workers were reported to Occupational Health Services in the province of Reggio Emilia (Italy). This prompted the Public Health Department to study the prevalence of the disease among milk-cow breeders involved in Parmigiano-Reggiano cheese production: who are the biggest hay users. A suitable questionnaire was sent to 1875 farmers in three of the six districts of the province. Half of them (935: 841 males, 94 females) answered; further contacts and medical history research revealed 33 case of "likely FL". Twenty-three (2 females) (10 "missing"), underwent pulmonary function tests, chest X-rays, precipitins tests against Saccharopolyspora Rectivirgula and other fungal antigens and (22 farmers) bronchoalveolar lavage (BAL). According to the "Società Italiana di Medicina del Lavoro e di Igiene Industriale" diagnostic standards, we found 20 subjects suffering from FL among farmers collecting hay in large cylindrical (round) bales, dried on field (2.6%) and among others still using small (traditional), prismatic bales (0.5%). The prevalence on the whole exposed population (6000-9000 people) was estimated between 1.5% and 3.0% (90-270 people); no difference was found in FL prevalence between flat and hilly or mountain areas; the method of collecting hay in big "round" bales, dried on field, seems to produce higher frequencies of FL cases if compared with the traditional ones (more frequent in mountain areas). The new hay packing methods, using forced air driers, are suggested as a possible solution.

Formulae Based on Biomathematics to Estimate the Standard Value of Fetal Growth of Japanese.

PubMed

Miyagi, Yasunari; Tada, Katsuhiko; Takayoshi, Riko; Oguni, Nobutsugu; Sato, Yasushi; Shibata, Maki; Kiyokawa, Machiko; Hashimoto, Tadashi; Takada, Tomoyoshi; Oda, Takashi; Miyake, Takahito

2018-04-01

We devised biomathematics-based formulae to estimate the standard values of fetal growth of Japanese after 22 weeks' gestation. The growth rates of bi-parietal diameter (BPD), abdominal circumference (AC), femur length (FL), and estimated fetal body weight (EFBW) at the time of gestation were assumed to be proportional to the product of the value at the time and the rest value of an unknown maximum value, respectively. The EFBW was also assumed to follow a multiple logistic function of BPD, AC and FL to fit the standard values of Japanese fetuses published by the Japan Society of Ultrasonics in Medicine. The Mann-Whitney test was used for statistical analysis. The values as a function of gestational day, t, were as follows: BPD(t)=99.6/(1+exp (2.725-0.01837＊t)) (mm); AC(t)=39.7/(1+exp (2.454-0.01379＊t)) (cm); FL(t)=79.6/(1+exp (2.851-0.01710＊t)) (mm); EFBW(t)=8045.1/(1+exp (6.028-0.06582＊BPD(t)-0.1469＊AC(t)+ 0.07377＊FL(t))) (g). EFBW as a function of BPD, AC and FL was as follows: EFBW=8045.1/(1+exp (4.747+ 0.02584＊BPD+0.1010＊AC-0.1416＊FL)) (g). When the BPD, AC and FL were at -2 standard deviation (SD), -1SD, mean and + 2SD, the EFBW values calculated by the formula were statistically closer to the standard values than conventional formulas with p-values of 4.871×10-7, 4.228×10-7, 9.777×10-7 and 0.028, respectively. The formulae based on biomathematics might be useful to estimate the fetal growth standard values.

Proposed Relocation of the 37th Tactical Fighter Wing and Other Tactical Force Structure Actions. Draft Environmental Impact Statement

DTIC Science & Technology

1991-02-05

finance , insurance and real estate FL flight level ft feet FWS U.S. Fish and Wildlife Service FWW Fighter Weapon Wing FY fiscal year GAF German Air Force...three locations are related to socioeconomics. Potential effects of the realignment on employment, income, public finance , housing, and local economic...Manufacturing 89 107 1 Transportation, communications, and utilities 135 202 2 * Wholesale and retail tradeb 514 594 5 3 Finance , insurance, and real estate 216

International Conference on Curves and Surfaces (4th), Saint-Malo, France, 1-7 July 1999. Proceedings, Volume 1. Curve and Surface Design

DTIC Science & Technology

2000-04-01

the Air Force grant issued by the European Aerospace Office of Research and Development. The United States has a royalty free license throughout the...UNIVERSITY PRESS Nashville fl9 ~Oc -O i U.S. Government Rights License This work relates to Department of the Air Force Grant/rContract issued by the...in engineering , medicine, and the sciences. SERIES EDITOR: Larry L. Schumaker Stevenson Professor of Mathematics Vanderbilt University PREVIOUSLY

Effect of tensile mean stress on fatigue behavior of single-crystal and directionally solidified superalloys

NASA Technical Reports Server (NTRS)

Kalluri, Sreeramesh; Mcgaw, Michael A.

1990-01-01

Two nickel base superalloys, single crystal PWA 1480 and directionally solidified MAR-M 246 + Hf, were studied in view of the potential usage of the former and usage of the latter as blade materials for the turbomachinery of the space shuttle main engine. The baseline zero mean stress (ZMS) fatigue life (FL) behavior of these superalloys was established, and then the effect of tensile mean stress (TMS) on their FL behavior was characterized. At room temperature these superalloys have lower ductilities and higher strengths than most polycrystalline engineering alloys. The cycle stress-strain response was thus nominally elastic in most of the fatigue tests. Therefore, a stress range based FL prediction approach was used to characterize both the ZMS and TMS fatigue data. In the past, several researchers have developed methods to account for the detrimental effect of tensile mean stress on the FL for polycrystalline engineering alloys. However, the applicability of these methods to single crystal and directionally solidified superalloys has not been established. In this study, these methods were applied to characterize the TMS fatigue data of single crystal PWA 1480 and directionally solidified MAR-M 246 + Hf and were found to be unsatisfactory. Therefore, a method of accounting for the TMS effect on FL, that is based on a technique proposed by Heidmann and Manson was developed to characterize the TMS fatigue data of these superalloys. Details of this method and its relationship to the conventionally used mean stress methods in FL prediction are discussed.

Fidgetin-like 2: a microtubule-based regulator of wound healing

PubMed Central

Charafeddine, Rabab A.; Makdisi, Joy; Schairer, David; O’Rourke, Brian P.; Diaz-Valencia, Juan D.; Chouake, Jason; Kutner, Allison; Krausz, Aimee; Adler, Brandon; Nacharaju, Parimala; Liang, Hongying; Mukherjee, Suranjana; Friedman, Joel M.; Friedman, Adam; Nosanchuk, Joshua D.; Sharp, David J.

2015-01-01

Wound healing is a complex process driven largely by the migration of a variety of distinct cell types from the wound margin into the wound zone. In this study, we identify the previously uncharacterized microtubule-severing enzyme, Fidgetin-like 2 (FL2), as a fundamental regulator of cell migration that can be targeted in vivo using nanoparticle-encapsulated siRNA to promote wound closure and regeneration. In vitro, depletion of FL2 from mammalian tissue culture cells results in a more than two-fold increase in the rate of cell movement, due in part to a significant increase in directional motility. Immunofluorescence analyses indicate that FL2 normally localizes to the cell edge, importantly to the leading edge of polarized cells, where it regulates the organization and dynamics of the microtubule cytoskeleton. To clinically translate these findings, we utilized a nanoparticle-based siRNA delivery platform to locally deplete FL2 in both murine full-thickness excisional and burn wounds. Topical application of FL2 siRNA nanoparticles to either wound type results in a significant enhancement in the rate and quality of wound closure both clinically and histologically relative to controls. Taken together, these results identify FL2 as a promising therapeutic target to promote the regeneration and repair of cutaneous wounds. PMID:25756798

Solid Waste Management in Marine Amphibious Force (MAF) Operations: Analysis and Alternatives.

DTIC Science & Technology

1980-09-01

Experience during the Southeast Asia conflict and elsewhere shows that MAF solid waste management requires a significant deployment of manpower and equipment...MAF varies, by necessity, with the location or type of military action. Based in part on recent experience gained in the Southeast Asia conflict, a...4 1- r 410 0 0 MCD %4 0. 0 0> 0 0 0.Z5 -4 4r44. ,4-14 1 cc U 44 UCI 4 4 0 -401. 4 0 0 U2fl󈧺 $ICɜ 41 *4 0J 4)% 4 4) u ~ .)ails- ii , )4 -4 to 40 be

Exploiting elasticity: Modeling the influence of neural control on mechanics and energetics of ankle muscle-tendons during human hopping.

PubMed

Robertson, Benjamin D; Sawicki, Gregory S

2014-07-21

We present a simplified Hill-type model of the human triceps surae-Achilles tendon complex working on a gravitational-inertial load during cyclic contractions (i.e. vertical hopping). Our goal was to determine the role that neural control plays in governing muscle, or contractile element (CE), and tendon, or series elastic element (SEE), mechanics and energetics within a compliant muscle-tendon unit (MTU). We constructed a 2D parameter space consisting of many combinations of stimulation frequency and magnitude (i.e. neural control strategies). We compared the performance of each control strategy by evaluating peak force and average positive mechanical power output for the system (MTU) and its respective components (CE, SEE), force-length (F-L) and -velocity (F-V) operating point of the CE during active force production, average metabolic rate for the CE, and both MTU and CE apparent efficiency. Our results suggest that frequency of stimulation plays a primary role in governing whole-MTU mechanics. These include the phasing of both activation and peak force relative to minimum MTU length, average positive power, and apparent efficiency. Stimulation amplitude was primarily responsible for governing average metabolic rate and within MTU mechanics, including peak force generation and elastic energy storage and return in the SEE. Frequency and amplitude of stimulation both played integral roles in determining CE F-L operating point, with both higher frequency and amplitude generally corresponding to lower CE strains, reduced injury risk, and elimination of the need for passive force generation in the CE parallel elastic element (PEE). Copyright © 2014 Elsevier Ltd. All rights reserved.

Influence of the target molecule on the oxygen radical absorbance capacity index: a comparison between alizarin red- and fluorescein-based methodologies.

PubMed

Martin, I; Aspée, A; Torres, P; Lissi, E; López-Alarcón, C

2009-12-01

A comparison of alizarin red (AR) and fluorescein (FL) as target molecules in oxygen radical absorbance capacity (ORAC)-like methods is reported. Galangin, apigenin, ferulic acid, and coumaric acid decreased AR initial consumption rate, whereas quercetin, kaempferol, luteolin, caffeic acid, and sinapic acid inhibited its consumption through an induction time, associated with a repair mechanism. On the other hand, all compounds protected FL with a clear induction time. AR was more selective and provides ORAC-AR values considerably smaller for compounds of low reactivity. The ORAC-AR value for luteolin was nearly 200 times that of coumaric acid. However, the ratio of ORAC-FL values for luteolin and coumaric acid was only 1.2. This different selectivity implies that AR provides ORAC values more related to reactivity than FL. ORAC-AR values of infusions were considerably smaller than the corresponding ORAC-FL values. These differences are interpreted in terms of the capacity of FL to generate induction times, irrespective of the reactivity of the additive. It is proposed that comparison of ORAC-AR and ORAC-FL values could afford a rough estimation of the average reactivity of the antioxidants titrated by the ORAC-FL methodology.

Pretreatment Circulating Serum Cytokines Associated with Follicular and Diffuse Large B-Cell Lymphoma: A Clinic-Based Case-Control Study

PubMed Central

Charbonneau, Bridget; Maurer, Matthew J; Ansell, Stephen M; Slager, Susan L; Fredericksen, Zachary S; Ziesmer, Steven C; Macon, William R; Habermann, Thomas M; Witzig, Thomas E; Link, Brian K; Cerhan, James R; Novak, Anne J

2012-01-01

Background Abnormal immune function is a key factor in predisposition to non-Hodgkin lymphoma (NHL). We evaluated the association of 30 cytokines individually and as a profile with diffuse large B-cell (DLBCL) and follicular (FL) lymphomas. Methods We used a multiplexed assay to measure 30 cytokine concentrations in pre-treatment serum in a case-control study of 234 FL, 188 DLBCL, and 400 control participants. Unconditional logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CI) adjusted for age and sex, and polytomous regression was used to evaluate heterogeneity between FL and DLBCL. Principal components analysis (PCA) was used to assess cytokine profiles associated with FL and DLBCL. Results In single cytokine modeling, we found that 12 of the 30 circulating serum cytokines were significantly (P<0.05) associated with FL and/or DLBCL after accounting for multiple testing (q<0.05). Soluble IL-2R (sIL-2R) had the strongest association with both FL (OR=6.0 for highest versus lowest tertile, 95% CI 3.8–9.5; p-trend=1.8 × 10−21) and DLBCL (OR=7.6, 95% CI 4.5–13.1; p-trend=7.2 × 10−20). IL1RA and IL-12p40 also showed similar associations for DLBCL and FL. In contrast, HGF, MIG, and MIP-1α had a stronger association with DLBCL compared to FL, and IL-6, IL-8, IL-10, IFN-γ, IP-10, and VEGF were only statistically significantly associated with DLBCL after accounting for multiple testing. However, in PCA modeling, a cytokine profile based on sIL-2R, IL-1RA, MIG, IP-10, IL-8, and IL-12p40 explained most of the variability between controls and both FL and DLBCL. Conclusions We identified some single cytokines unique to DLBCL, but overall cytokine associations were more similar than distinct for DLBCL and FL. While these data are limited by concerns of reverse causality, they do suggest cytokines and cytokine profiles that can be prioritized in future studies. PMID:23010502

THE FUTURE OF THE EUROPEAN AIR TRANSPORT COMMAND: AN ANALYSIS OF CAPABILITY ENHANCEMENTS FROM THE ITALIAN AIR FORCE AND AIR MOBILITY COMMAND

DTIC Science & Technology

2016-02-15

25 Bibliography Bowie, Christopher, Fred Frostic, Kevin Lewis, John Lunch, David Ochmanek, and Philip Proppe. The New Calculus : Analyzing...a critical analysis. Carlisle, Pennsylvania: US Army War College, class 2012. Stewart , Rory. The Place in Between. Orlando, FL: Harcourt Books

VIEW OF THE TOPS OF ALTITUDE CHAMBER R (TO LEFT) ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

VIEW OF THE TOPS OF ALTITUDE CHAMBER R (TO LEFT) AND ALTITUDE CHAMBER L (TO RIGHT) FROM THE 42’-0” LEVEL OF ACCESS PLATFORMS, FACING NORTHWEST - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy.

PubMed

Woodall, Benjamin P; Woodall, Meryl C; Luongo, Timothy S; Grisanti, Laurel A; Tilley, Douglas G; Elrod, John W; Koch, Walter J

2016-10-14

GRK2, a G protein-coupled receptor kinase, plays a critical role in cardiac physiology. Adrenergic receptors are the primary target for GRK2 activity in the heart; phosphorylation by GRK2 leads to desensitization of these receptors. As such, levels of GRK2 activity in the heart directly correlate with cardiac contractile function. Furthermore, increased expression of GRK2 after cardiac insult exacerbates injury and speeds progression to heart failure. Despite the importance of this kinase in both the physiology and pathophysiology of the heart, relatively little is known about the role of GRK2 in skeletal muscle function and disease. In this study we generated a novel skeletal muscle-specific GRK2 knock-out (KO) mouse (MLC-Cre:GRK2 fl/fl ) to gain a better understanding of the role of GRK2 in skeletal muscle physiology. In isolated muscle mechanics testing, GRK2 ablation caused a significant decrease in the specific force of contraction of the fast-twitch extensor digitorum longus muscle yet had no effect on the slow-twitch soleus muscle. Despite these effects in isolated muscle, exercise capacity was not altered in MLC-Cre:GRK2 fl/fl mice compared with wild-type controls. Skeletal muscle hypertrophy stimulated by clenbuterol, a β 2 -adrenergic receptor (β 2 AR) agonist, was significantly enhanced in MLC-Cre:GRK2 fl/fl mice; mechanistically, this seems to be due to increased clenbuterol-stimulated pro-hypertrophic Akt signaling in the GRK2 KO skeletal muscle. In summary, our study provides the first insights into the role of GRK2 in skeletal muscle physiology and points to a role for GRK2 as a modulator of contractile properties in skeletal muscle as well as β 2 AR-induced hypertrophy. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Assessment of femur length for fetal biometry in Malaysian pregnant women

NASA Astrophysics Data System (ADS)

Ramli, Ramzun Maizan; Adam, Noraina; Jaafar, Mohamad Suhaimi; Rahman, Azhar Abdul; Mustafa, Iskandar Shahrim; Radzi, Yasmin; Nordin, Suriani; Kamarudin, Izyani

2013-05-01

The fetal biometry assessment of femur length (FL) was developed in most countries to evaluate the foetus growth. In this study, the FL values for Malaysia population were determined. A prospective study was conducted and a number of 6501 pregnant women were involved with 12372 FL data were collected, performed at antenatal care clinic of Hospital Pulau Pinang, Malaysia. The SPSS software version 17 (regression analysis and paired sample t-test) were used for analysis. The FL values of this study were constructed and rapid FL growth rate in second trimester (2.59 mm/week) than third trimester (1.43 mm/week) was observed. By ethnicity, no significant difference (p ≥ 0.05) was found between the FL values for fetuses of Malay and Indian ethnic's mother (t = -2.042), however these two groups shows significantly (p < 0.05) higher values than those of Chinese ethnic's mother (t = 4.019, 4.083; accordingly). The FL values of Malaysian resulted as significant difference with the common reference FL values from USA and UK, and also with selected Asian populations (India, China, Korea and Japan). As conclusion, it is strong suggested that medical practitioner in Malaysia should avoid in using biometry references based on other population to avoid under- or over-estimation and for accurate assessment of the fetus growth. As this study involved local Malaysian fetuses, therefore the medical practitioner can use the FL values of this study as reference value for fetus biometry assessment in Malaysia.

Protein-ligand docking using fitness learning-based artificial bee colony with proximity stimuli.

PubMed

Uehara, Shota; Fujimoto, Kazuhiro J; Tanaka, Shigenori

2015-07-07

Protein-ligand docking is an optimization problem, which aims to identify the binding pose of a ligand with the lowest energy in the active site of a target protein. In this study, we employed a novel optimization algorithm called fitness learning-based artificial bee colony with proximity stimuli (FlABCps) for docking. Simulation results revealed that FlABCps improved the success rate of docking, compared to four state-of-the-art algorithms. The present results also showed superior docking performance of FlABCps, in particular for dealing with highly flexible ligands and proteins with a wide and shallow binding pocket.

Oxygen optical gas sensing by reversible fluorescence quenching in photo-oxidized poly(9,9-dioctylfluorene) thin films.

PubMed

Anni, M; Rella, R

2010-02-04

We investigated the fluorescence (FL) dependence on the environment oxygen content of poly(9,9-dioctylfluorene) (PF8) thin films. We show that the PF8 interactions with oxygen are not limited to the known irreversible photo-oxidation, resulting in the formation of Keto defects, but also reversible FL quenching is observed. This effect, which is stronger for the Keto defects than for the PF8, has been exploited for the realization of a prototype oxygen sensor based on FL quenching. The sensing sensitivity of Keto defects is comparable with the state of the art organic oxygen sensors based on phosphorescence quenching.

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA, John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA, John F. Kennedy Space Center, Florida. Drawing 79K05424, Seelye Stevenson Value & Knecht, March, 1975. SITE WORK, GENERAL AREA PLAN. Sheet 8 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING. NASA John F. Kennedy ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING. NASA John F. Kennedy Space Center, Florida. File Number 79K06740, NASA, November 1975. SPACE & WEIGHT ALLOCATION, ORBITER PATH IN TRANSFER AISLE. Sheet 6 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Programmed Control of Optical Grating Scales for Visual Research.

DTIC Science & Technology

1980-12-01

A -AOO .9 AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOO--ETC F/6 14/2 PROGRAMMED CONTROL OF OPTI CAL GRATING SCALES FOR VISUAL RESEARC --ETC(fl...custom system for AMRL. The cost in memory parts alone was $40,000, a good indication that the market is not over-priced. Ca-? western Reserve

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, John F. Kennedy Space Center, Florida. Drawing 68-K-L-11213, NASA KSC, November, 1968. CHAMBER “R” ELEVATION. Sheet 4 - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, John F. Kennedy Space Center, Florida. Drawing 68-K-L-11213, NASA KSC, November, 1968. WORK PLATFORM DETAIL. Sheet 6 - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, John F. Kennedy Space Center, Florida. Drawing 68-K-L-11213, NASA KSC, November, 1968. CHAMBER “L” ELEVATION. Sheet 3 - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Photocopy of drawing. LAUNCH COMPLEX 39. NASA, John F. Kennedy ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. LAUNCH COMPLEX 39. NASA, John F. Kennedy Space Center, Florida. File Number 203-100, Urbahn-Roberts-Seelye-Moran, October, 1963. LCC TRANSVERSE SECTIONS AA & BB. Sheet 29-45 - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. LAUNCH COMPLEX 39, CRAWLER TRANSPORTER. NASA, John ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. LAUNCH COMPLEX 39, CRAWLER TRANSPORTER. NASA, John F. Kennedy Space Center, Florida. Drawing 75M05760, KSC-Launch Support Equipment Engineering Division, January 1967. GENERAL ARRANGEMENT. Sheet 1 of 4 - Cape Canaveral Air Force Station, Launch Complex 39, Crawler Transporters, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Light Battalion Task Force Reconnaissance and Surveillance: Clear Vision or Groping in the Dark

DTIC Science & Technology

1990-12-23

1 gP.Oi. t@PO n b fl lot this colita n of information s etimated to a.’vae I hout car reions. including " tme for arviewing r r ct ,O matc ng eaiting...SUBTITLE S . FUNDING NUMBERS LIGHT BATTALION TASK FORCE RECONNAISSANCE AND SURVEILLANCE: CLEAR VISION OR GROPING IN TIHE DARK (U) 6. AUTHOR( S ) XAJ...DAVID B. LACQUEMENT 7. PERFORMING ORGANIZATION NAME( S ) AND ADDRESS(ES) B. PERFORMING ORGANIZATION SCHOOL OF ADVANCED MILITARY STUDIES REPORT NUMBER ATTN

Installation Restoration Program. Phase II. Confirmation/Quantification. Stage 1 for Air Force Plant 6, Cobb County, Georgia. Volume 3.

DTIC Science & Technology

1985-08-09

and regulations. Additional hazard areas refer to non-regulated operations and/or practices that pose potential risks to human and environmental ...ORGANI1A’iON REPORT NUMPERASE IRP-IIa-AFP6 64 xAfi OF PEROING ORG IS ZArTON Śo 7a.AM OF MONITORING OAYIZAToN Environmental Science ’ aeedwabi.) U.S. Air...Force and Engineering, Inc ca ed AORESS Y. State, And ZIP C017) AOSS Approvetaoe pblic reease; P. Box ESE Occupational and Environmental Gainesville, FL

Detection and Delineation of Oral Cancer With a PARP1-Targeted Optical Imaging Agent.

PubMed

Kossatz, Susanne; Weber, Wolfgang; Reiner, Thomas

2017-01-01

More sensitive and specific methods for early detection are imperative to improve survival rates in oral cancer. However, oral cancer detection is still largely based on visual examination and histopathology of biopsy material, offering no molecular selectivity or spatial resolution. Intuitively, the addition of optical contrast could improve oral cancer detection and delineation, but so far no molecularly targeted approach has been translated. Our fluorescently labeled small-molecule inhibitor PARPi-FL binds to the DNA repair enzyme poly(ADP-ribose)polymerase 1 (PARP1) and is a potential diagnostic aid for oral cancer delineation. Based on our preclinical work, a clinical phase I/II trial opened in March 2017 to evaluate PARPi-FL as a contrast agent for oral cancer imaging. In this commentary, we discuss why we chose PARP1 as a biomarker for tumor detection and which particular characteristics make PARPi-FL an excellent candidate to image PARP1 in optically guided applications. We also comment on the potential benefits of our molecularly targeted PARPi-FL-guided imaging approach in comparison to existing oral cancer screening adjuncts and mention the adaptability of PARPi-FL imaging to other environments and tumor types.

False Lumen Flow Patterns and their Relation with Morphological and Biomechanical Characteristics of Chronic Aortic Dissections. Computational Model Compared with Magnetic Resonance Imaging Measurements

PubMed Central

Segers, Patrick; Pineda, Victor; Cuellar, Hug; García-Dorado, David; Evangelista, Arturo

2017-01-01

Aortic wall stiffness, tear size and location and the presence of abdominal side branches arising from the false lumen (FL) are key properties potentially involved in FL enlargement in chronic aortic dissections (ADs). We hypothesize that temporal variations on FL flow patterns, as measured in a cross-section by phase-contrast magnetic resonance imaging (PC-MRI), could be used to infer integrated information on these features. In 33 patients with chronic descending AD, instantaneous flow profiles were quantified in the FL at diaphragm level by PC-MRI. We used a lumped-parameter model to assess the changes in flow profiles induced by wall stiffness, tear size/location, and the presence of abdominal side branches arising from the FL. Four characteristic FL flow patterns were identified in 31/33 patients (94%) based on the direction of flow in systole and diastole: BA = systolic biphasic flow and primarily diastolic antegrade flow (n = 6); BR = systolic biphasic flow and primarily diastolic retrograde flow (n = 14); MA = systolic monophasic flow and primarily diastolic antegrade flow (n = 9); MR = systolic monophasic flow and primarily diastolic retrograde flow (n = 2). In the computational model, the temporal variation of flow directions within the FL was highly dependent on the position of assessment along the aorta. FL flow patterns (especially at the level of the diaphragm) showed their characteristic patterns due to variations in the cumulative size and the spatial distribution of the communicating tears, and the incidence of visceral side branches originating from the FL. Changes in wall stiffness did not change the temporal variation of the flows whereas it importantly determined intraluminal pressures. FL flow patterns implicitly codify morphological information on key determinants of aortic expansion in ADs. This data might be taken into consideration in the imaging protocol to define the predictive value of FL flows. PMID:28125720

Rapid Estimation of Astaxanthin and the Carotenoid-to-Chlorophyll Ratio in the Green Microalga Chromochloris zofingiensis Using Flow Cytometry.

PubMed

Chen, Junhui; Wei, Dong; Pohnert, Georg

2017-07-19

The green microalga Chromochloris zofingiensis can accumulate significant amounts of valuable carotenoids, mainly natural astaxanthin, a product with applications in functional food, cosmetics, nutraceuticals, and with potential therapeutic value in cardiovascular and neurological diseases. To optimize the production of astaxanthin, it is essential to monitor the content of astaxanthin in algal cells during cultivation. The widely used HPLC (high-performance liquid chromatography) method for quantitative astaxanthin determination is time-consuming and laborious. In the present work, we present a method using flow cytometry (FCM) for in vivo determination of the astaxanthin content and the carotenoid-to-chlorophyll ratio (Car/Chl) in mixotrophic C. zofingiensis . The method is based on the assessment of fluorescent characteristics of cellular pigments. The mean fluorescence intensity (MFI) of living cells was determined by FCM to monitor pigment formation based on the correlation between MFI detected in particular channels (FL1: 533 ± 15 nm; FL2: 585 ± 20 nm; FL3: >670 nm) and pigment content in algal cells. Through correlation and regression analysis, a linear relationship was observed between MFI in FL2 (band-pass filter, emission at 585 nm in FCM) and astaxanthin content (in HPLC) and applied for predicting astaxanthin content. With similar procedures, the relationships between MFI in different channels and Car/Chl ratio in mixotrophic C. zofingiensis were also determined. Car/Chl ratios could be estimated by the ratios of MFI (FL1/FL3, FL2/FL3). FCM is thus a highly efficient and feasible method for rapid estimation of astaxanthin content in the green microalga C. zofingiensis . The rapid FCM method is complementary to the current HPLC method, especially for rapid evaluation and prediction of astaxanthin formation as it is required during the high-throughput culture in the laboratory and mass cultivation in industry.

Rapid Estimation of Astaxanthin and the Carotenoid-to-Chlorophyll Ratio in the Green Microalga Chromochloris zofingiensis Using Flow Cytometry

PubMed Central

Chen, Junhui; Pohnert, Georg

2017-01-01

The green microalga Chromochloris zofingiensis can accumulate significant amounts of valuable carotenoids, mainly natural astaxanthin, a product with applications in functional food, cosmetics, nutraceuticals, and with potential therapeutic value in cardiovascular and neurological diseases. To optimize the production of astaxanthin, it is essential to monitor the content of astaxanthin in algal cells during cultivation. The widely used HPLC (high-performance liquid chromatography) method for quantitative astaxanthin determination is time-consuming and laborious. In the present work, we present a method using flow cytometry (FCM) for in vivo determination of the astaxanthin content and the carotenoid-to-chlorophyll ratio (Car/Chl) in mixotrophic C. zofingiensis. The method is based on the assessment of fluorescent characteristics of cellular pigments. The mean fluorescence intensity (MFI) of living cells was determined by FCM to monitor pigment formation based on the correlation between MFI detected in particular channels (FL1: 533 ± 15 nm; FL2: 585 ± 20 nm; FL3: >670 nm) and pigment content in algal cells. Through correlation and regression analysis, a linear relationship was observed between MFI in FL2 (band-pass filter, emission at 585 nm in FCM) and astaxanthin content (in HPLC) and applied for predicting astaxanthin content. With similar procedures, the relationships between MFI in different channels and Car/Chl ratio in mixotrophic C. zofingiensis were also determined. Car/Chl ratios could be estimated by the ratios of MFI (FL1/FL3, FL2/FL3). FCM is thus a highly efficient and feasible method for rapid estimation of astaxanthin content in the green microalga C. zofingiensis. The rapid FCM method is complementary to the current HPLC method, especially for rapid evaluation and prediction of astaxanthin formation as it is required during the high-throughput culture in the laboratory and mass cultivation in industry. PMID:28753934

Application of Discrete Guidance and Control Theory to Future Army Modular Missiles.

DTIC Science & Technology

1980-09-30

30 April - I May, Dr. Pastrick and Dr. Seltzer-attended the 1980 Tactical Missiles Conference at Eglin Air Force Base. !7 t ’ I 9 * I. - , Ji ...1.7140E+00 P2= 9.0903E- 61 WnTm 1.8221E+00 DET=-1.8519E-03 P1= I.7093E+00 P2= 9.0568E-01 SWrT= 1.850E+00 DET=-1.8416E-03 Piz 1.7055E+00 P2= 9.0290E-01 * WrT...fl-n~-~a~ 4 - - I I I TECHNICAL REPORT T-79-64 I SAMPLED-DATA ANALYSIS IN PARAMETER SPACE S.M. Seltzer Technology Laboratory June 1979 Ji j edet•one

Absorption and emission spectroscopic characterization of photo-dynamics of photoactivated adenylyl cyclase mutant bPAC-Y7F of Beggiatoa sp.

PubMed

Penzkofer, Alfons; Stierl, Manuela; Mathes, Tilo; Hegemann, Peter

2014-11-01

The photoactivated cyclase bPAC of the microbial mats bacterium Beggiatoa sp. consists of a BLUF domain and an adenylyl cyclase domain. It has strong activity of photo-induced cyclic adenylyl monophosphate (cAMP) formation and is therefore an important optogenetic tool in neuroscience applications. The SUMO-bPAC-Y7F mutant where Tyr-7 is replaced by Phe-7 in the BLUF domain has lost the typical BLUF domain photo-cycle dynamics. Instead, the investigated SUMO-bPAC-Y7F mutant consisted of three protein conformations with different triplet based photo-dynamics: (i) reversible flavin quinone (Fl) cofactor reduction to flavin semiquinone (FlH), (ii) reversible violet/near ultraviolet absorbing flavin photoproduct (FlA) formation, and (iii) irreversible red absorbing flavin photoproduct (FlC) formation. Absorption and emission spectroscopic measurements on SUMO-bPAC-Y7F were carried out before, during and after light exposure. Flavin photo-dynamics schemes are developed for the SUMO-bPAC-Y7F fractions performing photo-induced FlH, FlA, and FlC formation. Quantitative parameters of the flavin cofactor excitation, relaxation and recovery dynamics in SUMO-bPAC-Y7F are determined. Copyright © 2014 Elsevier B.V. All rights reserved.

Anoctamin 1 contributes to inflammatory and nerve-injury induced hypersensitivity.

PubMed

Lee, Byeongjun; Cho, Hawon; Jung, Jooyoung; Yang, Young Duk; Yang, Dong-Jin; Oh, Uhtaek

2014-01-23

Various pathological conditions such as inflammation or injury can evoke pain hypersensitivity. That represents the response to innocuous stimuli or exaggerated response to noxious stimuli. The molecular mechanism based on the pain hypersensitivity is associated with changes in many of ion channels in dorsal-root ganglion (DRG) neurons. Anoctamin 1 (ANO1/TMEM16A), a Ca2+ activated chloride channel is highly visible in small DRG neurons and responds to heat. Mice with an abolished function of ANO1 in DRG neurons demonstrated attenuated pain-like behaviors when exposed to noxious heat, suggesting a role in acute thermal nociception. In this study, we further examined the function of ANO1 in mediating inflammation- or injury-induced hyperalgesia or allodynia. Using Advillin/Ano1fl/fl (Adv/Ano1fl/fl) mice that have a functional ablation of Ano1 mainly in DRG neurons, we were able to determine its role in mediating thermal hyperalgesia and mechanical allodynia induced by inflammation or nerve injury. The thermal hyperalgesia and mechanical allodynia induced by carrageenan injection and spared-nerve injury were significantly reduced in Adv/Ano1fl/fl mice. In addition, flinching or licking behavior after bradykinin or formalin injection was also significantly reduced in Adv/Ano1fl/fl mice. Since pathological conditions augment nociceptive behaviors, we expected ANO1's contribution to the excitability of DRG neurons. Indeed, the application of inflammatory mediators reduced the threshold for action potential (rheobase) or time for induction of the first action potential in DRG neurons isolated from control (Ano1fl/fl) mice. These parameters for neuronal excitability induced by inflammatory mediators were not changed in Adv/Ano1fl/fl mice, suggesting an active contribution of ANO1 in augmenting the neuronal excitability. In addition to ANO1's role in mediating acute thermal pain as a heat sensor, ANO1 is also capable of augmenting the excitability of DRG neurons under inflammatory or neuropathic conditions and thereby aggravates inflammation- or tissue injury-induced pathological pain.

Damage evaluation in graphene underlying atomic layer deposition dielectrics

PubMed Central

Tang, Xiaohui; Reckinger, Nicolas; Poncelet, Olivier; Louette, Pierre; Ureña, Ferran; Idrissi, Hosni; Turner, Stuart; Cabosart, Damien; Colomer, Jean-François; Raskin, Jean-Pierre; Hackens, Benoit; Francis, Laurent A.

2015-01-01

Based on micro-Raman spectroscopy (μRS) and X-ray photoelectron spectroscopy (XPS), we study the structural damage incurred in monolayer (1L) and few-layer (FL) graphene subjected to atomic-layer deposition of HfO2 and Al2O3 upon different oxygen plasma power levels. We evaluate the damage level and the influence of the HfO2 thickness on graphene. The results indicate that in the case of Al2O3/graphene, whether 1L or FL graphene is strongly damaged under our process conditions. For the case of HfO2/graphene, μRS analysis clearly shows that FL graphene is less disordered than 1L graphene. In addition, the damage levels in FL graphene decrease with the number of layers. Moreover, the FL graphene damage is inversely proportional to the thickness of HfO2 film. Particularly, the bottom layer of twisted bilayer (t-2L) has the salient features of 1L graphene. Therefore, FL graphene allows for controlling/limiting the degree of defect during the PE-ALD HfO2 of dielectrics and could be a good starting material for building field effect transistors, sensors, touch screens and solar cells. Besides, the formation of Hf-C bonds may favor growing high-quality and uniform-coverage dielectric. HfO2 could be a suitable high-K gate dielectric with a scaling capability down to sub-5-nm for graphene-based transistors. PMID:26311131

77 FR 42425 - Amendment of Air Traffic Service (ATS) Routes in the Vicinity of Vero Beach, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-19

... Orlando, FL, 140[deg] radials; Orlando; Ocala, FL; Cross City, FL; Greenville, FL; Pecan, GA; Eufaula, AL... Virginia Key 014[deg] and Treasure, FL, 143[deg] radials; Treasure; INT Treasure 296[deg] and Orlando, FL, 162[deg] radials; Orlando; Ocala, FL; Cross City, FL; to Seminole, FL. The portion outside the United...

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. ALL PLATFORMS-ARCHITECTURAL, GENERAL ARRANGEMENT, EAST-WEST ELEVATIONS. Sheet 12 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. LAUNCH COMPLEX 39. NASA, John F. Kennedy ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. LAUNCH COMPLEX 39. NASA, John F. Kennedy Space Center, Florida. File Number 203-100, Urbahn-Roberts-Seelye-Moran, October, 1963. LCC FLOOR 3, LEVEL 38’-0”, AREA “P”. Sheet 29-39 - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VAB HB2 & 4 ET HOLDING CELLS, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VAB HB-2 & 4 ET HOLDING CELLS, UPGRADE TO CHECKOUT CELLS. NASA John F. Kennedy Space Center, Florida. File Number 79K33180, Reynolds, Smith & Hill, February 1991. GENERAL ARRANGEMENT, PLAN & ELEVATIONS. Sheet 4 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VAB HB2 & 4 ET HOLDING CELLS, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VAB HB-2 & 4 ET HOLDING CELLS, UPGRADE TO CHECKOUT CELLS. NASA John F. Kennedy Space Center, Florida. File Number 79K33180, Reynolds, Smith & Hill, February 1991. HINGED PANEL EQUIPMENT SCHEDULE. Sheet 28 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Hoph Bifurcation in Viscous, Low Speed Flows About an Airfoil with Structural Coupling

DTIC Science & Technology

1993-03-01

8 2.1 Equations of Motion ...... ..................... 8 2.2 Coordinate Transformation ....................... 13 2.3 Aerodynamic...a-frame) f - Apparent body forces applied in noninertial system fL - Explicit fourth-order numerical damping term Ai - Implicit fourth-order...resulting airfoil motion . The equations describing the airfoil motion are integrated in time using a fourth-order Runge-Kutta algorithm. The

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. ALTITUDE CHAMBERS “L” & “R” STRUCTURES. NASA, John F. Kennedy Space Center, Florida. Drawing 68-K-L-11213, NASA KSC, March, 1971. DOOR LATCH MECHANISM & DOOR LATCHING RATCHET. Sheet 14 - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Photocopy of drawing. LAUNCH COMPLEX 39. NASA John F. Kennedy ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. LAUNCH COMPLEX 39. NASA John F. Kennedy Space Center, Florida. File Number 203-100, Urbahn-Roberts-Seelye-Moran, October 1963. VERTICAL ASSEMBLY BUILDING, LOW BAY, SECTIONS J-J, K-K, & L-L. Sheet 33-32 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. LAUNCH COMPLEX 39. NASA, John F. Kennedy ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. LAUNCH COMPLEX 39. NASA, John F. Kennedy Space Center, Florida. File Number 203-100, Urbahn-Roberts-Seelye-Moran, October, 1963. VOLUME 29, LAUNCH CONTROL CENTER (LCC) TITLE AND LOCATION SHEET. Sheet 29-01 - Cape Canaveral Air Force Station, Launch Complex 39, Launch Control Center, LCC Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

When Military Fitness Standards No Longer Apply: The High Prevalence of Metabolic Syndrome in Recent Air Force Retirees

DTIC Science & Technology

2017-08-27

Kissimee, FL, 27-30 August 2017 {Poster & Presentation) in accordance with MDWI 41-108, has been approved and assigned local fi le #17207. 2...6 TRUE.MARK.W.1119949757 ~~~:’.·:_:....-...:. ---·-’" -- 21. APPROVING AUTHORITY’S PRINTED NAME, RANK, TITLE 22. APPROVING AUTHORITY’S SIGNATURE Wi

Applied Meteorology Unit - Operational Contributions to Spaceport Canaveral

NASA Technical Reports Server (NTRS)

Bauman, William H., III; Roeder, William P.; Lafosse, Richard A.; Sharp, David W.; Merceret, Francis J.

2004-01-01

The Applied Meteorology Unit (AMU) provides technology development, evaluation and transition services to improve operational weather support to the Space Shuttle and the National Space Program. It is established under a Memorandum of Understanding among NASA, the Air Force and the National .Weather Service (NWS). The AMU is funded and managed by NASA and operated by ENSCO, Inc. through a competitively awarded NASA contract. The primary customers are the 45th Weather Squadron (45WS) at Cape Canaveral Air Force Station (CCAFS), FL; the Spaceflight Meteorology Group (SMG) at Johnson Space Center (JSC) in Houston, TX; and the NWS office in Melbourne, FL (NWS MLB). This paper will briefly review the AMU's history and describe the three processes through which its work is assigned. Since its inception in 1991 the AMU has completed 72 projects, all of which are listed at the end of this paper. At least one project that highlights each of the three tasking processes will be briefly reviewed. Some of the projects that have been especially beneficial to the space program will also be discussed in more detail, as will projects that developed significant new techniques or science in applied meteorology.

Silver nanoparticles-enhanced time-resolved fluorescence sensor for VEGF(165) based on Mn-doped ZnS quantum dots.

PubMed

Zhu, Dong; Li, Wei; Wen, Hong-Mei; Yu, Sheng; Miao, Zhao-Yi; Kang, An; Zhang, Aihua

2015-12-15

A silver nanoparticles (AgNPs)-enhanced time-resolved fluorescence (TR-FL) sensor based on long-lived fluorescent Mn-doped ZnS quantum dots (QDs) is developed for the sensitive detection of vascular endothelial growth factor-165 (VEGF165), a predominant cancer biomarker in cancer angiogenesis. The aptamers bond with the Mn-doped ZnS QDs and the BHQ-2 quencher-labelling strands hybridized in duplex are coupled with streptavidin (SA)-functionalized AgNPs to form the AgNPs-enhanced TR-FL sensor, showing lower fluorescence intensity in the duplex state due to the fluorescence resonance energy transfer (FRET) between the Mn-doped ZnS QDs and quenchers. Upon the addition of VEGF165, the BHQ-2 quencher-labelling strands of the duplex are displaced, leading to the disruption of the FRET. As a result, the fluorescence of the Mn-doped QDs within the proximity of the AgNPs is recovered. The FL signal can be measured free of the interference of short-lived background by setting appropriate delay time and gate time, which offers a signal with high signal-to-noise ratio in photoluminescent biodetection. Compared with the bare TR-FL sensor, the AgNPs-based TR-FL sensor showed a huge improvement in fluorescence based on metal-enhanced fluorescence (MEF) effect, and the sensitivity increased 11-fold with the detection limit of 0.08 nM. In addition, the sensor provided a wide range of linear detection from 0.1 nM to 16 nM. Copyright © 2015 Elsevier B.V. All rights reserved.

Ozonation-based decolorization of food dyes for recovery of fruit leather wastes.

PubMed

Zhu, Wenda; Koziel, Jacek A; Cai, Lingshuang; Brehm-Stecher, Byron F; Ozsoy, H Duygu; van Leeuwen, J Hans

2013-08-28

Commercial manufacture of fruit leathers (FL) usually results in a portion of the product that is out of specification. The disposition of this material poses special challenges in the food industry. Because the material remains edible and contains valuable ingredients (fruit pulp, sugars, acidulates, etc.), an ideal solution would be to recover this material for product rework. A key practical obstacle to such recovery is that compositing of differently colored wastes results in an unsalable gray product. Therefore, a safe and scalable method for decolorization of FL prior to product rework is needed. This research introduces a novel approach utilizing ozonation for color removal. To explore the use of ozonation as a decolorization step, we first applied it to simple solutions of the commonly used food colorants 2-naphthalenesulfonic acid (Red 40), tartrazine (Yellow 5), and erioglaucine (Blue 1). Decolorization was measured by UV/vis spectrometry at visible wavelengths and with a Hunter colorimeter. Volatile and semivolatile byproducts from ozone-based colorant decomposition were identified and quantified with solid phase microextraction coupled with gas chromatography-mass spectrometry (SPME-GC-MS). Removal of Yellow 5, Red 40 and Blue 1 of about 65%, 80%, and 90%, respectively, was accomplished with 70 g of ozone applied per 1 kg of redissolved and resuspended FL. Carbonyl compounds were identified as major byproducts from ozone-induced decomposition of the food colorants. A conservative risk assessment based on quantification results and published toxicity information of potentially toxic byproducts, determined that ozone-based decolorization of FL before recycling is acceptable from a safety standpoint. A preliminary cost estimate based on recycling of 1000 tons of FL annually suggests a potential of $275,000 annual profit from this practice at one production facility alone.

Targeted Reduction of Vascular Msx1 and Msx2 Mitigates Arteriosclerotic Calcification and Aortic Stiffness in LDLR-Deficient Mice Fed Diabetogenic Diets

PubMed Central

Cheng, Su-Li; Behrmann, Abraham; Shao, Jian-Su; Ramachandran, Bindu; Krchma, Karen; Bello Arredondo, Yoanna; Kovacs, Attila; Mead, Megan; Maxson, Robert

2014-01-01

When fed high-fat diets, male LDLR−/− mice develop obesity, hyperlipidemia, hyperglycemia, and arteriosclerotic calcification. An osteogenic Msx-Wnt regulatory program is concomitantly upregulated in the vasculature. To better understand the mechanisms of diabetic arteriosclerosis, we generated SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR−/− mice, assessing the impact of Msx1+Msx2 gene deletion in vascular myofibroblast and smooth muscle cells. Aortic Msx2 and Msx1 were decreased by 95% and 34% in SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR−/− animals versus Msx1(fl/fl);Msx2(fl/fl);LDLR−/− controls, respectively. Aortic calcium was reduced by 31%, and pulse wave velocity, an index of stiffness, was decreased in SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR−/− mice vs. controls. Fasting blood glucose and lipids did not differ, yet SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR−/− siblings became more obese. Aortic adventitial myofibroblasts from SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR−/− mice exhibited reduced osteogenic gene expression and mineralizing potential with concomitant reduction in multiple Wnt genes. Sonic hedgehog (Shh) and Sca1, markers of aortic osteogenic progenitors, were also reduced, paralleling a 78% reduction in alkaline phosphatase (TNAP)–positive adventitial myofibroblasts. RNA interference revealed that although Msx1+Msx2 supports TNAP and Wnt7b expression, Msx1 selectively maintains Shh and Msx2 sustains Wnt2, Wnt5a, and Sca1 expression in aortic adventitial myofibroblast cultures. Thus, Msx1 and Msx2 support vascular mineralization by directing the osteogenic programming of aortic progenitors in diabetic arteriosclerosis. PMID:25056439

Monitoring the growth of polyoxomolybdate nanoparticles in suspension by flow field-flow fractionation.

PubMed

Chen, Bailin; Jiang, Huijian; Zhu, Yan; Cammers, Arthur; Selegue, John P

2005-03-30

We follow the evolution of polyoxomolybdate nanoparticles in suspensions derived from the keplerate (NH4)42[MoVI72MoV60O372(CH3CO2)30(H2O)72].ca..300H2O.ca..10CH3CO2NH4 ({Mo132}) by flow field-flow fractionation (FlFFF) to monitor the particle-size distribution in situ, atomic force and high-resolution transmission electron microscopy (AFM, SEM, and HRTEM) to confirm particle sizes, inductively coupled plasma-optical emission spectrometry (ICP-OES) to determine the Mo content of the FlFFF-separated fractions, and UV/visible spectroscopy to confirm the identity of the species in suspension. We observe the formation of 3-75-nm polyoxomolybdate particles in suspension and the dynamic growth of {Mo132} crystals.

Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY® FL-labeled probe or primer

PubMed Central

Kurata, Shinya; Kanagawa, Takahiro; Yamada, Kazutaka; Torimura, Masaki; Yokomaku, Toyokazu; Kamagata, Yoichi; Kurane, Ryuichiro

2001-01-01

We have developed a simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY® FL fluorescence was quenched by its interaction with a uniquely positioned guanine. This approach makes use of an oligonucleotide probe or primer containing a BODIPY® FL-modified cytosine at its 5′-end. When such a probe was hybridized with a target DNA, its fluorescence was quenched by the guanine in the target, complementary to the modified cytosine, and the quench rate was proportional to the amount of target DNA. This widely applicable technique will be used directly with larger samples or in conjunction with the polymerase chain reaction to quantify small DNA samples. PMID:11239011

Approximate, computationally efficient online learning in Bayesian spiking neurons.

PubMed

Kuhlmann, Levin; Hauser-Raspe, Michael; Manton, Jonathan H; Grayden, David B; Tapson, Jonathan; van Schaik, André

2014-03-01

Bayesian spiking neurons (BSNs) provide a probabilistic interpretation of how neurons perform inference and learning. Online learning in BSNs typically involves parameter estimation based on maximum-likelihood expectation-maximization (ML-EM) which is computationally slow and limits the potential of studying networks of BSNs. An online learning algorithm, fast learning (FL), is presented that is more computationally efficient than the benchmark ML-EM for a fixed number of time steps as the number of inputs to a BSN increases (e.g., 16.5 times faster run times for 20 inputs). Although ML-EM appears to converge 2.0 to 3.6 times faster than FL, the computational cost of ML-EM means that ML-EM takes longer to simulate to convergence than FL. FL also provides reasonable convergence performance that is robust to initialization of parameter estimates that are far from the true parameter values. However, parameter estimation depends on the range of true parameter values. Nevertheless, for a physiologically meaningful range of parameter values, FL gives very good average estimation accuracy, despite its approximate nature. The FL algorithm therefore provides an efficient tool, complementary to ML-EM, for exploring BSN networks in more detail in order to better understand their biological relevance. Moreover, the simplicity of the FL algorithm means it can be easily implemented in neuromorphic VLSI such that one can take advantage of the energy-efficient spike coding of BSNs.

Magainin 2a - Induced Permeabilization of Phospholipid Vesicles

DTIC Science & Technology

1991-01-02

flJ ə) fl) C rH -H 0) E M D e t e 1 0 0 % • 43 • fl) J3 0 C3...fl) +) ft-H to U4 EH RJ OP 43 o 4J H -H IS *M 0 0 fl) c to 0 • H O 4J O -H in 73 TJ h RJ 0 MH >t 43 fl) to TJ flj fl) fl) C...r H - H F r e t e r m O fl) VD TJ tp to G rt •rH r% f o l l o w : f 6 C F 1 O 43 0) to T J flJ fl) fl) C rH •H fl) E Ul

Storyline-Based Videogames in the FL Classroom

ERIC Educational Resources Information Center

Casañ-Pitarch, Ricardo

2017-01-01

The use of videogames in the foreign language (FL) classroom seems to be gradually increasing nowadays. TICs are making the lives of educators easier and their teaching methods more effective; these positive experiences make that researchers in this field are constantly introducing and developing new teaching methods and electronic applications.…

Establishment of highly metastatic KRAS mutant lung cancer cell sublines in long-term three-dimensional low attachment cultures

PubMed Central

Nakano, Tomoyuki; Kanai, Yoshihiko; Amano, Yusuke; Yoshimoto, Taichiro; Matsubara, Daisuke; Shibano, Tomoki; Tamura, Tomoko; Oguni, Sachiko; Katashiba, Shizuka; Ito, Takeshi; Murakami, Yoshinori; Fukayama, Masashi; Murakami, Takashi; Endo, Shunsuke; Niki, Toshiro

2017-01-01

Decreased cell-substratum adhesion is crucially involved in metastasis. Previous studies demonstrated that lung cancer with floating cell clusters in histology is more likely to develop metastasis. In the present study, we investigated whether cancer cells in long-term, three-dimensional low attachment cultures acquire high metastatic potential; these cells were then used to examine the mechanisms underlying metastasis. Two KRAS-mutated adenocarcinoma cell lines (A549 and H441) were cultured and selected on ultra-low attachment culture dishes, and the resulting cells were defined as FL (for floating) sublines. Cancer cells were inoculated into NOD/SCID mice via an intracardiac injection, and metastasis was evaluated using luciferase-based imaging and histopathology. In vitro cell growth (in attachment or suspension cultures), migration, and invasion were assayed. A whole genomic analysis was performed to identify key molecular alterations in FL sublines. Upon detachment on low-binding dishes, parental cells initially formed rounded spheroids with limited growth activity. However, over time in cultures, cells gradually formed smaller spheroids that grew slowly, and, after 3–4 months, we obtained FL sublines that regained prominent growth potential in suspension cultures. On ordinary dishes, FL cells reattached and exhibited a more spindle-shaped morphology than parental cells. No marked differences were observed in cell growth with attachment, migration, or invasion between FL sublines and parental cell lines; however, FL cells exhibited markedly increased growth potential under suspended conditions in vitro and stronger metastatic abilities in vivo. A genomic analysis identified epithelial-mesenchymal transition (EMT) and c-Myc amplification in A549-FL and H441-FL cells, respectively, as candidate mechanisms for metastasis. The growth potential of FL cells was markedly inhibited by lentiviral ZEB1 knockdown in A549-FL cells and by the inhibition of c-Myc through lentiviral knockdown or the pharmacological inhibitor JQ1 in H441-FL cells. Long-term three-dimensional low attachment cultures may become a useful method for investigating the mechanisms underlying metastasis mediated by decreased cell-substratum adhesion. PMID:28786996

Amphibian antimicrobial peptide fallaxin analogue FL9 affects virulence gene expression and DNA replication in Staphylococcus aureus.

PubMed

Gottschalk, Sanne; Gottlieb, Caroline T; Vestergaard, Martin; Hansen, Paul R; Gram, Lone; Ingmer, Hanne; Thomsen, Line E

2015-12-01

The rapid rise in antibiotic-resistant pathogens is causing increased health concerns, and consequently there is an urgent need for novel antimicrobial agents. Antimicrobial peptides (AMPs), which have been isolated from a wide range of organisms, represent a very promising class of novel antimicrobials. In the present study, the analogue FL9, based on the amphibian AMP fallaxin, was studied to elucidate its mode of action and antibacterial activity against the human pathogen Staphylococcus aureus. Our data showed that FL9 may have a dual mode of action against S. aureus. At concentrations around the MIC, FL9 bound DNA, inhibited DNA synthesis and induced the SOS DNA damage response, whereas at concentrations above the MIC the interaction between S. aureus and FL9 led to membrane disruption. The antibacterial activity of the peptide was maintained over a wide range of NaCl and MgCl(2) concentrations and at alkaline pH, while it was compromised by acidic pH and exposure to serum. Furthermore, at subinhibitory concentrations of FL9, S. aureus responded by increasing the expression of two major virulence factor genes, namely the regulatory rnaIII and hla, encoding α-haemolysin. In addition, the S. aureus-encoded natural tolerance mechanisms included peptide cleavage and the addition of positive charge to the cell surface, both of which minimized the antimicrobial activity of FL9. Our results add new information about FL9 and its effect on S. aureus, which may aid in the future development of analogues with improved therapeutic potential.

Applied Meteorology Unit (AMU) Quarterly Report Fourth Quarter FY-13

NASA Technical Reports Server (NTRS)

Bauman, William; Crawford, Winifred; Watson, Leela; Shafer, Jaclyn; Huddleston, Lisa

2013-01-01

Ms. Shafer completed the task to determine relationships between pressure gradients and peak winds at Vandenberg Air Force Base (VAFB), and began developing a climatology for the VAFB wind towers; Dr. Huddleston completed the task to develop a tool to help forecast the time of the first lightning strike of the day in the Kennedy Space Center (KSC)/Cape Canaveral Air Force Station (CCAFS) area; Dr. Bauman completed work on a severe weather forecast tool focused on the Eastern Range (ER), and also developed upper-winds analysis tools for VAFB and Wallops Fl ight Facility (WFF); Ms. Crawford processed and displayed radar data in the software she will use to create a dual-Doppler analysis over the east-central Florida and KSC/CCAFS areas; Mr. Decker completed developing a wind pairs database for the Launch Services Program to use when evaluating upper-level winds for launch vehicles; Dr. Watson continued work to assimilate observational data into the high-resolution model configurations she created for WFF and the ER.

Structure of a seismogenic fault zone in dolostones: the Foiana Line (Italian Southern Alps)

NASA Astrophysics Data System (ADS)

Di Toro, G.; Fondriest, M.; Smith, S. A.; Aretusini, S.

2012-12-01

Fault zones in carbonate rocks (limestones and dolostones) represent significant upper crustal seismogenic sources in several areas worldwide (e.g. L'Aquila 2009 Mw = 6.3 in central Italy). Here we describe an exhumed example of a regionally-significant fault zone cutting dolostones. The Foiana Line (FL) is a major NNE-SSW-trending sinistral transpressive fault cutting sedimentary Triassic dolostones in the Italian Southern Alps. The FL has a cumulative vertical throw of 1.5-2 km that reduces toward its southern termination. The fault zone is 50-300 m wide and is exposed for ~ 10 km along strike within several outcrops exhumed from increasing depths from the south (1 km) to the north (2.5 km). The southern portion of the FL consists of heavily fractured (shattered) dolostones, with particles of a few millimeters in size (exposed in badlands topography over an area of 6 km2), cut by a dense network of 1-20 m long mirror-like fault surfaces with dispersed attitudes. The mirror-like faults have mainly dip-slip reverse kinematics and displacements ranging between 0.04 m and 0.5 m. The northern portion of the FL consists of sub-parallel fault strands spaced 2-5 m apart, surrounded by 2-3 m thick bands of shattered dolostones. The fault strands are characterized by smooth to mirror-like sub-vertical slip surfaces with dominant strike-slip kinematics. Overall, deformation is more localized moving from South to North along the FL. Mirror-like fault surfaces similar to those found in the FL were produced in friction experiments at the deformation conditions expected during seismic slip along the FL (Fondriest et al., this meeting). Scanning Electron Microscope investigations of the natural shattered dolostones beneath the mirror-like fault surfaces show: 1) lack of significant shear strain (even at a few micrometers from the slip surface), 2) pervasive extensional fracturing down to the micrometer scale, 3) exploded clasts with radial fractures, and 4) chains of split clasts (resulting from force chains) oriented at 60-80 degrees to the slip surfaces. Similar features have been reported in natural and experimental pulverized rocks, the latter produced under dynamic stress wave loading conditions. 3-Dimensional rupture simulations along strike-slip faults predict (1) off-fault damage distributions with "flower-like" shapes (broad damage zone near the surface that rapidly narrows with increasing confining pressure, e.g., Ma and Andrews, 2010) and (2) formation of secondary faults/fractures with disperse attitudes and kinematics near the surface, with horizontal slip at depth. Qualitatively, these theoretical results compare favorably with increasing strain localization and a switch in fault kinematics recognized along the FL moving from the southern, shallower portion of the fault zone to the northern, deeper portion. Observations along the FL suggest that the association of shattered dolostones and mirror-like slip surfaces might be a potential indicator of seismic rupture propagation. Further detailed structural mapping along the FL coupled with experimental work on rock pulverization will be necessary to understand rupture dynamics in dolostones.

Targeted reduction of vascular Msx1 and Msx2 mitigates arteriosclerotic calcification and aortic stiffness in LDLR-deficient mice fed diabetogenic diets.

PubMed

Cheng, Su-Li; Behrmann, Abraham; Shao, Jian-Su; Ramachandran, Bindu; Krchma, Karen; Bello Arredondo, Yoanna; Kovacs, Attila; Mead, Megan; Maxson, Robert; Towler, Dwight A

2014-12-01

When fed high-fat diets, male LDLR(-/-) mice develop obesity, hyperlipidemia, hyperglycemia, and arteriosclerotic calcification. An osteogenic Msx-Wnt regulatory program is concomitantly upregulated in the vasculature. To better understand the mechanisms of diabetic arteriosclerosis, we generated SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR(-/-) mice, assessing the impact of Msx1+Msx2 gene deletion in vascular myofibroblast and smooth muscle cells. Aortic Msx2 and Msx1 were decreased by 95% and 34% in SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR(-/-) animals versus Msx1(fl/fl);Msx2(fl/fl);LDLR(-/-) controls, respectively. Aortic calcium was reduced by 31%, and pulse wave velocity, an index of stiffness, was decreased in SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR(-/-) mice vs. controls. Fasting blood glucose and lipids did not differ, yet SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR(-/-) siblings became more obese. Aortic adventitial myofibroblasts from SM22-Cre;Msx1(fl/fl);Msx2(fl/fl);LDLR(-/-) mice exhibited reduced osteogenic gene expression and mineralizing potential with concomitant reduction in multiple Wnt genes. Sonic hedgehog (Shh) and Sca1, markers of aortic osteogenic progenitors, were also reduced, paralleling a 78% reduction in alkaline phosphatase (TNAP)-positive adventitial myofibroblasts. RNA interference revealed that although Msx1+Msx2 supports TNAP and Wnt7b expression, Msx1 selectively maintains Shh and Msx2 sustains Wnt2, Wnt5a, and Sca1 expression in aortic adventitial myofibroblast cultures. Thus, Msx1 and Msx2 support vascular mineralization by directing the osteogenic programming of aortic progenitors in diabetic arteriosclerosis. © 2014 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘E’, ROOF PLAN, ARCHITECTURAL. Sheet 22 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, 2ND FLOOR PLAN, ARCHITECTURAL. Sheet 38 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 39 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘E’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 23 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, ROOF PLAN, ARCHITECTURAL. Sheet 36 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘D’, 3RD FLOOR PLAN, ARCHITECTURAL. Sheet 37 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘B’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 30 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘C’, 2ND FLOOR PLAN, ARCHITECTURAL. Sheet 15 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘C’, ROOF PLAN, ARCHITECTURAL. Sheet 14 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘B’, ROOF PLAN, ARCHITECTURAL. Sheet 28 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘C’, MAIN FLOOR PLAN, ARCHITECTURAL. Sheet 16 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. HIGH BAY 3, EXTENSIBLE WORK PLATFORM ‘B’, 2ND FLOOR PLAN, ARCHITECTURAL. Sheet 29 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. TRANSFER AISLE NORTH DOOR,ARCHITECTURAL NORTH ELEVATION AND MISC. DETAILS. Sheet 78 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Project Summary: Biology-Inspired Autonomous Control

DTIC Science & Technology

2011-02-01

AUTHOR( S ) 5d. PROJECT NUMBER 2304 Johnny H. Evers 5e. TASK NUMBER AM 5f. WORK UNIT NUMBER 08 7. PERFORMING...ORGANIZATION NAME( S ) AND ADDRESS(ES) Air Force Research Laboratory, Munitions Directorate 8. PERFORMING ORGANIZATION REPORT NUMBER AFRL/RWAV...101 West Eglin Blvd Eglin AFB, FL 32542-6810 AFRL-RW-EG-TP-2011-006 9. SPONSORING / MONITORING AGENCY NAME( S ) AND ADDRESS(ES

Environmental Assessment for the Advanced Extremely High Frequency Satellite Beddown and Deployment Program

DTIC Science & Technology

2010-07-01

Final Environmental Assessment 22 Several invasive exotic plant species are also found on the station , particularly in disturbed areas such as...Department of Transportation EA Environmental Assessment Ec Debris Casualty Area EELV Evolved Expendable Launch Vehicle EIS Environmental Impact...Canaveral Air Force Station (CCAFS) in Florida (FL). This Environmental Assessment (EA) documents the results of a study of the potential

Photocopy of drawing. SPACECRAFT INDUSTRIAL AREA, O&C BUILDING, A&T AREA, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. SPACECRAFT INDUSTRIAL AREA, O&C BUILDING, A&T AREA, ALTITUDE CHAMBER “L”. NASA, John F. Kennedy Space Center, Florida. Drawing 79K00213, U.S. Army Corps of Engineers, no date. SHELL PENETRATION SCHEDULE. Sheet 4 - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VAB HB2 & 4 ET HOLDING CELLS, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VAB HB-2 & 4 ET HOLDING CELLS, UPGRADE TO CHECKOUT CELLS. NASA John F. Kennedy Space Center, Florida. File Number 79K33180, Reynolds, Smith & Hill, February 1991. H.B.-2, PLANS AT LEVELS 4,5,&6. Sheet 7 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VAB HB2 & 4 ET HOLDING CELLS, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VAB HB-2 & 4 ET HOLDING CELLS, UPGRADE TO CHECKOUT CELLS. NASA John F. Kennedy Space Center, Florida. File Number 79K33180, Reynolds, Smith & Hill, February 1991. H.B.-2, PLANS AT LEVELS 1,2,&3. Sheet 6 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. VAB HB2 & 4 ET HOLDING CELLS, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VAB HB-2 & 4 ET HOLDING CELLS, UPGRADE TO CHECKOUT CELLS. NASA John F. Kennedy Space Center, Florida. File Number 79K33180, Reynolds, Smith & Hill, February 1991. H.B.-2, PLANS AT LEVELS 7,8,&9. Sheet 8 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. SPACECRAFT INDUSTRIAL AREA, O&C BUILDING, A&T AREA, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. SPACECRAFT INDUSTRIAL AREA, O&C BUILDING, A&T AREA, ALTITUDE CHAMBER “L”. NASA, John F. Kennedy Space Center, Florida. Drawing 79K00213, U.S. Army Corps of Engineers, no date. SHELL PENETRATION LAYOUT. Sheet 3 - Cape Canaveral Air Force Station, Launch Complex 39, Altitude Chambers, First Street, between Avenue D and Avenue E, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, SECTIONS I. Sheet A17 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, SECTIONS II. Sheet A18 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. ISOMETRIC: EXISTING ML NO. 3 LAUNCHER. Sheet A1 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, SECTIONS IV. Sheet A20 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. ISOMETRIC VIEW: MLP NO. 1. Sheet A10 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Development of Site Characterization Simulator Specifications

DTIC Science & Technology

1996-11-01

Jeff Farrar, Geotechnical Engineer with Earth Sciences Laboratory, Bureau of Reclamation; Jason Smolensky, Hydrogeologist at SRK-Canada, and Doctors Ed...Heyse and Mark Goltz , Department of Engineering and Environmental Management, Air Force Institute of Technology). Considering these discussions, the...Windows 3.1 or higher, 35mb, hard disk. Delta Research Corporation, Niceville FL. 139 Roberts, P.V., Goltz , M.N., and Mackay, D.M. 1986. A Natural

Healthy Dietary Patterns and Oxidative Stress as Measured by Fluorescent Oxidation Products in Nurses' Health Study.

PubMed

Jung, Seungyoun; Smith-Warner, Stephanie A; Willett, Walter C; Wang, Molin; Wu, Tianying; Jensen, Majken; Hankinson, Susan E; Eliassen, A Heather

2016-09-21

Healthy diets may lower oxidative stress and risk of chronic diseases. However, no previous studies examined associations between diet and fluorescent oxidation products (FlOP), a global marker of oxidative stress. We evaluated associations between healthy eating patterns (Alternative Healthy Eating Index (AHEI), Dietary Approach to Stop Hypertension (DASH), and Alternate Mediterranean Diet (aMED)) and FlOP, measured at three excitation/emission wavelengths (FlOP_360, FlOP_320, FlOP_400) from 2021 blood samples collected from 1688 women within the Nurses' Health Study. AHEI, DASH, and aMED scores were significantly positively associated with FlOP_360 and FlOP_320 concentrations ( p -trend ≤ 0.04), but not associated with FlOP_400. Among specific food groups that contribute to these diet scores, significantly positive associations were observed with legumes and vegetables for FlOP_360, vegetables and fruits for FlOP_320, and legumes and alcohol for FlOP_400. Inverse associations were observed with nuts, sweets or desserts, and olive oil for FlOP_360, nuts for FlOP_320 and sweets or desserts for FlOP_400 (all p -trend ≤ 0.05). However, FlOP variation due to diet was small compared to overall FlOP variation. In conclusion, AHEI, DASH, and aMED scores were unexpectedly positively, but weakly, associated with FlOP_360 and FlOP_320. However, these findings should be interpreted cautiously as the determinants of FlOP concentrations are not fully understood.

NaEuF4/Au@Ag2S nanoparticles-based fluorescence resonant transfer DNA sensor for ultrasensitive detection of DNA energy.

PubMed

Liu, Yuhong; Zhao, Linlin; Zhang, Jin; Zhang, Jinzha; Zhao, Wenbo; Mao, Chun

2016-12-01

The work investigates a new fluorescence resonance energy transfer (FRET) system using NaEuF 4 nanoparticles (NPs) and Au@Ag 2 S NPs as the energy donor-acceptor pair for the first time. The NaEuF 4 /Au@Ag 2 S NPs-based FRET DNA sensor was constructed with NaEuF 4 NPs as the fluorescence (FL) donor and Au@Ag 2 S core-shell NPs as FL acceptor. In order to find the matching energy acceptor, the amount of AgNO 3 and Na 2 S were controlled in the synthesis process to overlap the absorption spectrum of energy acceptor with the emission spectrum of energy donors. The sensitivity of FRET-based DNA sensor can be enhanced and the self-absorption of ligand as well as the background of signals can be decreased because of Eu 3+ which owns large Stokes shifts and narrow emission bands due to f-f electronic transitions of 4f shell. We obtained the efficient FRET system by studying suitable distance between the donor and acceptor. Then the FRET-based DNA sensor was used for the design of specific and sensitive detection of target DNA and the quenching efficiency (ΔFL/F 0 , ΔFL=F-F 0 ) of FL was logarithmically related to the concentration of the target DNA, ranging from 100aM to 100pM. We can realize an ultrasensitive detection of target DNA with a detection limit of 32 aM. This proposed method was feasible to analyse target DNA in real samples with satisfactory results. Copyright © 2016 Elsevier B.V. All rights reserved.

A Task-Based Language Teaching Approach to Developing Metacognitive Strategies for Listening Comprehension

ERIC Educational Resources Information Center

Chou, Mu-Hsuan

2017-01-01

In second (L2) or foreign language (FL) learning, learning strategies help learners perform tasks, solve specific problems, and compensate for learning deficits. Of the strategy types, metacognitive strategies manage and regulate the construction of L2 or FL knowledge. Although learning strategies are frequently taught via teacher demonstration,…

Fuel Load (FL)

Treesearch

Duncan C. Lutes; Robert E. Keane

2006-01-01

The Fuel Load method (FL) is used to sample dead and down woody debris, determine depth of the duff/ litter profile, estimate the proportion of litter in the profile, and estimate total vegetative cover and dead vegetative cover. Down woody debris (DWD) is sampled using the planar intercept technique based on the methodology developed by Brown (1974). Pieces of dead...

Manual of Articulatory Phonetics: Teacher's Guide.

ERIC Educational Resources Information Center

Smalley, William A.

This teaching guide is closely integrated with the "Manual of Articulatory Phonetics" (FL 002 882) and its "Workbook Supplement" (FL 002 881). The guide is based on lesson plans which have been developed by the staff using the manual during its developmental period. An introduction on using the lesson plans and teaching techniques is provided.…

Metabolic fingerprinting of fresh lymphoma samples used to discriminate between follicular and diffuse large B-cell lymphomas.

PubMed

Barba, Ignasi; Sanz, Carolina; Barbera, Angels; Tapia, Gustavo; Mate, José-Luis; Garcia-Dorado, David; Ribera, Josep-Maria; Oriol, Albert

2009-11-01

To investigate if proton nuclear magnetic resonance ((1)H NMR) spectroscopy-based metabolic profiling was able to differentiate follicular lymphoma (FL) from diffuse large B-cell lymphoma (DLBCL) and to study which metabolites were responsible for the differences. High-resolution (1)H NMR spectra was obtained from fresh samples of lymph node biopsies obtained consecutively at one center (14 FL and 17 DLBCL). Spectra were processed using pattern-recognition methods. Discriminant models were able to differentiate between the two tumor types with a 86% sensitivity and a 76% specificity; the metabolites that most contributed to the discrimination were a relative increase of alanine in the case of DLBCL and a relative increase of taurine in FL. Metabolic models had a significant but weak correlation with Ki67 expression (r(2)=0.42; p=0.002) We have proved that it is possible to differentiate between FL and DLBCL based on their NMR metabolic profiles. This approach may potentially be applicable as a noninvasive tool for diagnostic and treatment follow-up in the clinical setting using conventional magnetic resonance systems.

An analytical model for subsurface irradiance and remote sensing reflectance in deep and shallow case-2 waters.

PubMed

Albert, A; Mobley, C

2003-11-03

Subsurface remote sensing signals, represented by the irradiance re fl ectance and the remote sensing re fl ectance, were investigated. The present study is based on simulations with the radiative transfer program Hydrolight using optical properties of Lake Constance (German: Bodensee) based on in-situ measurements of the water constituents and the bottom characteristics. Analytical equations are derived for the irradiance re fl ectance and remote sensing re fl ectance for deep and shallow water applications. The input of the parameterization are the inherent optical properties of the water - absorption a(lambda) and backscattering bb(lambda). Additionally, the solar zenith angle thetas, the viewing angle thetav , and the surface wind speed u are considered. For shallow water applications the bottom albedo RB and the bottom depth zB are included into the parameterizations. The result is a complete set of analytical equations for the remote sensing signals R and Rrs in deep and shallow waters with an accuracy better than 4%. In addition, parameterizations of apparent optical properties were derived for the upward and downward diffuse attenuation coefficients Ku and Kd.

Fluorene-Based Two-Dimensional Covalent Organic Framework with Thermoelectric Properties through Doping.

PubMed

Wang, Liangying; Dong, Bin; Ge, Rile; Jiang, Fengxing; Xu, Jingkun

2017-03-01

Organic semiconductors have great potential as flexible thermoelectric materials. A fluorene-based covalent organic framework (FL-COF-1) was designed with the aim of creating an enhanced π-π interaction among the crystalline backbones. By the introduction of fluorene units into the frameworks, the FL-COF-1 had high thermal stability with a BET surface area over 1300 m 2 g -1 . The open frameworks were favorable for doping with iodine and followed with the improved charge carrier mobility. The compressed pellet of I 2 @FL-COF-1 exhibited a high Seebeck coefficient of 2450 μV K -1 and power factor of 0.063 μW m -1 K -2 at room temperature, giving the first example of COFs' potential application as thermoelectric materials.

Determination of the ratio between mercaptalbumin and nonmercaptalbumin by HPLC with fluorescence probe specifically binding to albumin.

PubMed

Ohyama, Kaname; Kishikawa, Naoya; Matsuo, Aya; Imazato, Takahiro; Ueki, Yukitaka; Wada, Mitsuhiro; Nakashima, Kenichiro; Kuroda, Naotaka

2014-01-01

A simple and selective HPLC-fluorescence (FL) method with FL probe, 4-[4-(4-dimethylaminophenyl)-5-phenyl-1H-imidazol-2-yl]benzoic acid methyl ester (DAPIM), for simultaneous determination of mercaptalbumin (HMA) and nonmercaptalbumin (HNA1) was developed. After HMA and HNA1 were separated on an ion-exchange column, they were on-line and post-column mixed with DAPIM. The DAPIM-albumin complex produces FL (λex 370nm and λem 510nm); however, DAPIM solution never gives the FL. Based on this mechanism, selective determination of HMA and HNA1 were achieved without any pretreatment and interfering peak. The proposed method was applied to the measurement of HMA and HNA1 in human serum of healthy volunteers and diabetes mellitus patients. Copyright © 2013 Elsevier B.V. All rights reserved.

Air Force construction automation/robotics

NASA Technical Reports Server (NTRS)

Nease, AL; Dusseault, Christopher

1994-01-01

The Air Force has several unique requirements that are being met through the development of construction robotic technology. The missions associated with these requirements place construction/repair equipment operators in potentially harmful situations. Additionally, force reductions require that human resources be leveraged to the maximum extent possible and that more stringent construction repair requirements push for increased automation. To solve these problems, the U.S. Air Force is undertaking a research and development effort at Tyndall AFB, FL to develop robotic teleoperation, telerobotics, robotic vehicle communications, automated damage assessment, vehicle navigation, mission/vehicle task control architecture, and associated computing environment. The ultimate goal is the fielding of robotic repair capability operating at the level of supervised autonomy. The authors of this paper will discuss current and planned efforts in construction/repair, explosive ordnance disposal, hazardous waste cleanup, fire fighting, and space construction.

Retrospective analysis of first-line treatment for follicular lymphoma based on outcomes and medical economics.

PubMed

Muneishi, Manaka; Nakamura, Ayaka; Tachibana, Katsumi; Suemitsu, Junko; Hasebe, Shinji; Takeuchi, Kazuto; Yakushijin, Yoshihiro

2018-04-01

Follicular lymphoma (FL) is the most common type of non-Hodgkin lymphoma (NHL), with indolent progression. Several treatment options are selected, based not only on disease status, quality of life (QOL), and age of patient, but also on recent increasing medical costs. We retrospectively analysed the first-line treatment of FL with regard to treatment outcomes and medical economics, and discuss the appropriate strategies for FL. Data on a total of 69 newly-diagnosed patients with FL was retrospectively collected from 2001 to 2015. The median age of the patients was 60 years and the median follow-up was 58 months. A total of 25 cases with FL were treated with R monotherapy, and 28 cases were treated with R-CHOP as first-line treatment. The factors affecting the decision of physicians to use R or R-CHOP treatment were serum level of lactate dehydrogenase (LDH) and disease stage. The first-line treatment-associated survival did not show any statistical differences between R and R-CHOP. The average hospitalization and average of all medical costs during the first-line treatment were 4.1 days (R) versus 55.7 days (R-CHOP), and JPY 1,707,693 (USD 15,324) (R) versus JPY 2,136,117 (USD 19,170) (R-CHOP), respectively. R monotherapy for patients whose diseases show low tumor burden and who are not candidates for local treatment has benefits as a first-line treatment compared to R-CHOP, based on the patients' QOL and medical economics.

Changes in Functional Lung Regions During the Course of Radiation Therapy and Their Potential Impact on Lung Dosimetry for Non-Small Cell Lung Cancer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meng, Xue; Department of Radiation Oncology, Shandong Cancer Hospital, Shandong University, Jinan; Frey, Kirk

2014-05-01

Purpose: To study changes in functional activity on ventilation (V)/perfusion (Q) single-photon emission computed tomography (SPECT) during radiation therapy (RT) and explore the impact of such changes on lung dosimetry in patients with non-small cell lung cancer (NSCLC). Methods and Materials: Fifteen NSCLC patients with centrally located tumors were enrolled. All patients were treated with definitive RT dose of ≥60 Gy. V/Q SPECT-CT scans were performed prior to and after delivery of 45 Gy of fractionated RT. SPECT images were used to define temporarily dysfunctional regions of lung caused by tumor or other potentially reversible conditions as B3. The functional lung (FL)more » was defined on SPECT by 2 separate approaches: FL1, a threshold of 30% of the maximum uptake of the patient's lung; and FL2, FL1 plus B3 region. The impact of changes in FL between initiation of RT and delivery of 45 Gy on lung dosimetry were analyzed. Results: Fourteen patients (93%) had larger FL2 volumes than FL1 pre-RT (P<.001). Dysfunctional lung became functional in 11 patients (73%) on V SPECT and in 10 patients (67%) on Q SPECT. The dosimetric parameters generated from CT-based anatomical lung had significantly lower values in FL1 than FL2, with a median reduction in the volume of lung receiving a dose of at least 20 Gy (V{sub 20}) of 3%, 5.6%, and mean lung dose of 0.95 and 1.55 on V and Q SPECT respectively. Conclusions: Regional ventilation and perfusion function improve significantly during RT in centrally located NSCLC. Lung dosimetry values vary notably between different definitions of functional lung.« less

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. VEHICLE ASSEMBLY BUILDING MODIFICATIONS, HIGH BAY AREA. NASA John F. Kennedy Space Center, Florida. File Number 79K05424, Seelye Stevenson Value & Knecht, March 1975. TRANSFER AISLE NORTH DOOR, ARCHITECTURAL AND STRUCTURAL ELEVATIONS, SECTIONS AND DETAILS. Sheet 79 of 207 - Cape Canaveral Air Force Station, Launch Complex 39, Vehicle Assembly Building, VAB Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Special Operations Forces Language and Culture Needs Assessment: Language Resources And Self-Study

DTIC Science & Technology

2010-11-12

2007; Freitas & Levine, 2004; Tuzun, 2007). SOFLO should analyze and consider the cost-benefit of providing more instructors/tutors. “I would like...instructor is generally considered an effective training solution (Blunt, 2007; Freitas & Levine, 2004; Tuzun, 2007). Additionally, some SOF operators...Simulation and Education Conference: Orlando, Fl. Freitas , S., & Levene, M. (2004). An investigation of the use of simulations and video gaming for

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, ELEVATION – SIDE 1 & 2. Sheet A15 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, PLAN – DECK A. Sheet A13 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, ELEVATION – SIDE 3 & 4. Sheet A16 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, PLAN – DECK B. Sheet A14 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photocopy of drawing. MODIFICATIONS TO CONVERT ML NO. 3 TO MOBILE LAUNCHER PLATFORM NO. 1. NASA, John F. Kennedy Space Center, Florida. Drawing 79K04401, Reynolds, Smith and Hills, March, 1975. GENERAL ARRANGEMENT, MLP NO. 1, PLAN – DECK 0. Sheet A12 - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

United States Air Force Summary, Eighth Edition

DTIC Science & Technology

1982-01-01

Community•••,~••••• 85 80 80 41 65 84 100 82 99 96 USSR and Eastern Europe " ’ t ~, 35 o o 5 o 45 15 5 o o N... I v Bauxite Copper Nickel Lead Zinc...Boggs, Undy (LA) Benjamin, Adam Jr. (IN) Dicks, Norman D. (WA) McHugh , Matthew F. (NY) Ginn, Bo (GA) Lehman, William (FL) Hightower, Jock (TX) Sabo

New host record of five Flavobacterium species associated with tropical fresh water farmed fishes from North India

PubMed Central

Verma, Dev Kumar; Rathore, Gaurav

2015-01-01

Abstract Yellow pigmented, filamentous, Gram-negative bacteria belonging to genus Flavobacterium are commonly associated with infections in stressed fish. In this study, inter-species diversity of Flavobacterium was studied in apparently healthy freshwater farmed fishes. For this, ninety one yellow pigmented bacteria were isolated from skin and gill samples (n = 38) of three farmed fish species i.e. Labeo rohita, Catla catla and Cyprinus carpio. Among them, only twelve bacterial isolates (13.18%) were identified as Flavobacterium spp. on the basis of morphological, biochemical tests, partial 16S rDNA gene sequencing and phylogenetic analysis. On the basis of 16S rDNA gene sequencing, all the 12 isolates were 97.6-100% similar to six different formally described species of genus Flavobacterium. The 16S rDNA based phylogenetic analysis grouped these strains into six different clades. Of the 12 isolates, six strains (Fl9S1-6) grouped with F. suncheonense, two strains (Fl6I2, Fl6I3) with F. indicum and the rest four strains (Fl1A1, Fl2G1, Fl3H1 and Fl10T1) clustered with F. aquaticum, F. granuli, F. hercynium and F. terrae, respectively. None of these species except, F. hercynium were previously reported from fish. All the isolated Flavobacterium species possessed the ability of adhesion and biofilm formation to colonize the external surface of healthy fish. The present study is the first record of tropical freshwater farmed fishes as hosts to five environmentally associated species of the Flavobacterium. PMID:26691454

30 CFR 7.86 - Test equipment and specifications.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) Filters—F1, F2, F3, and F4; (ii) Flowmeters—FL1, FL2, FL3, FL4, FL5, FL6, and FL7; (iii) Upstream Gauges... equivalent results. The components are designated as follows— (i) Filters—F1, F2, F3, and F4; (ii) Flowmeters... exhaust pipe up to the filter holder, which are in contact with raw and diluted exhaust gas, shall be...

Towards an understanding of the relationship of functional literacy and numeracy to geographical health inequalities.

PubMed

Moon, Graham; Aitken, Grant; Roderick, Paul; Fraser, Simon; Rowlands, Gill

2015-10-01

The relative contributions of functional literacy and functional numeracy to health disparities remain poorly understood in developed world contexts. We seek to unpack their distinctive contributions and to examine how these contributions are framed by place-based deprivation and rurality. We present a multilevel logistic analysis of the 2011 Skills for Life Survey (SfLS), a representative governmental survey of adults aged 16-65 in England. Outcome measures were self-assessed health status and the presence of self-reported long-term health conditions. Exposure variables were functional literacy (FL) and functional numeracy (FN). Age, sex, individual socio-economic status, ethnicity, whether English was a first language, non-UK birthplaces, housing tenure and geography were included as potential confounders and mediators. Geography was measured as area-based deprivation and urban/rural status. FL and FN were both independently associated with self-assessed health status, though the association attenuated after taking account of confounders and mediators. For long-term conditions, the association with FN remained significant following inclusion of confounders and mediators whilst FL attenuated to non-significance. Rurality did not influence these associations. Area deprivation was a significant factor in attenuating the association between FL and self-assessed health status. Policy makers and health professionals will need to be aware of the distinctive impact of FN as well as FL when combating health inequalities, promoting health and managing long-term conditions. Copyright © 2015 Elsevier Ltd. All rights reserved.

Retrotransposon-Based Molecular Markers for Analysis of Genetic Diversity within the Genus Linum

PubMed Central

Melnikova, Nataliya V.; Kudryavtseva, Anna V.; Zelenin, Alexander V.; Lakunina, Valentina A.; Yurkevich, Olga Yu.; Speranskaya, Anna S.; Dmitriev, Alexey A.; Krinitsina, Anastasia A.; Belenikin, Maxim S.; Uroshlev, Leonid A.; Snezhkina, Anastasiya V.; Sadritdinova, Asiya F.; Koroban, Nadezda V.; Amosova, Alexandra V.; Samatadze, Tatiana E.; Guzenko, Elena V.; Lemesh, Valentina A.; Savilova, Anastasya M.; Rachinskaia, Olga A.; Kishlyan, Natalya V.; Rozhmina, Tatiana A.; Bolsheva, Nadezhda L.; Muravenko, Olga V.

2014-01-01

SSAP method was used to study the genetic diversity of 22 Linum species from sections Linum, Adenolinum, Dasylinum, Stellerolinum, and 46 flax cultivars. All the studied flax varieties were distinguished using SSAP for retrotransposons FL9 and FL11. Thus, the validity of SSAP method was demonstrated for flax marking, identification of accessions in genebank collections, and control during propagation of flax varieties. Polymorphism of Fl1a, Fl1b, and Cassandra insertions were very low in flax varieties, but these retrotransposons were successfully used for the investigation of Linum species. Species clusterization based on SSAP markers was in concordance with their taxonomic division into sections Dasylinum, Stellerolinum, Adenolinum, and Linum. All species of sect. Adenolinum clustered apart from species of sect. Linum. The data confirmed the accuracy of the separation in these sections. Members of section Linum are not as closely related as members of other sections, so taxonomic revision of this section is desirable. L. usitatissimum accessions genetically distant from modern flax cultivars were revealed in our work. These accessions are of utmost interest for flax breeding and introduction of new useful traits into flax cultivars. The chromosome localization of Cassandra retrotransposon in Linum species was determined. PMID:25243121

Organization - NOAA's National Weather Service

Science.gov Websites

Virginia Charleston Alabama Birmingham Huntsville Mobile Arkansas Little Rock Florida Jacksonville Key West Mobile/Pensacola Tallahassee, FL Key West, FL Miami-South Florida, FL Tampa Bay, FL Melbourne, FL

An integrated PCR colony hybridization approach to screen cDNA libraries for full-length coding sequences.

PubMed

Pollier, Jacob; González-Guzmán, Miguel; Ardiles-Diaz, Wilson; Geelen, Danny; Goossens, Alain

2011-01-01

cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) is a commonly used technique for genome-wide expression analysis that does not require prior sequence knowledge. Typically, quantitative expression data and sequence information are obtained for a large number of differentially expressed gene tags. However, most of the gene tags do not correspond to full-length (FL) coding sequences, which is a prerequisite for subsequent functional analysis. A medium-throughput screening strategy, based on integration of polymerase chain reaction (PCR) and colony hybridization, was developed that allows in parallel screening of a cDNA library for FL clones corresponding to incomplete cDNAs. The method was applied to screen for the FL open reading frames of a selection of 163 cDNA-AFLP tags from three different medicinal plants, leading to the identification of 109 (67%) FL clones. Furthermore, the protocol allows for the use of multiple probes in a single hybridization event, thus significantly increasing the throughput when screening for rare transcripts. The presented strategy offers an efficient method for the conversion of incomplete expressed sequence tags (ESTs), such as cDNA-AFLP tags, to FL-coding sequences.

MIP models for connected facility location: A theoretical and computational study☆

PubMed Central

Gollowitzer, Stefan; Ljubić, Ivana

2011-01-01

This article comprises the first theoretical and computational study on mixed integer programming (MIP) models for the connected facility location problem (ConFL). ConFL combines facility location and Steiner trees: given a set of customers, a set of potential facility locations and some inter-connection nodes, ConFL searches for the minimum-cost way of assigning each customer to exactly one open facility, and connecting the open facilities via a Steiner tree. The costs needed for building the Steiner tree, facility opening costs and the assignment costs need to be minimized. We model ConFL using seven compact and three mixed integer programming formulations of exponential size. We also show how to transform ConFL into the Steiner arborescence problem. A full hierarchy between the models is provided. For two exponential size models we develop a branch-and-cut algorithm. An extensive computational study is based on two benchmark sets of randomly generated instances with up to 1300 nodes and 115,000 edges. We empirically compare the presented models with respect to the quality of obtained bounds and the corresponding running time. We report optimal values for all but 16 instances for which the obtained gaps are below 0.6%. PMID:25009366

Morphological tuned preparation of zinc oxide: reduced graphene oxide composites for non-enzymatic fluorescence glucose sensing and enhanced photocatalysis

NASA Astrophysics Data System (ADS)

Sivalingam, Muthu Mariappan; Balasubramanian, Karthikeyan

2016-07-01

Zinc oxide: reduced graphene oxide (ZnO:rgo) composites with varying ZnO morphologies have been synthesized towards the application of non-enzymatic fluorescence (FL) glucose sensor and photocatalysis for methylene blue (MB) degradation. The phase structure of ZnO has confirmed by X-ray diffraction studies, and the band gap calculations were done by UV absorption spectra. Scanning electron microscope and Raman spectra revealed the morphological change and the vibrational studies of the prepared samples, respectively. The quenching of the FL emission band of ZnO:rgo composite sample confirmed the transfer of electrons from ZnO to rgo which inhibit the exciton recombination process. The non-enzymatic FL glucose sensing was carried out by the addition of dextrose glucose ( d-glucose) into the ZnO:rgo composite solution, which shows strong relationship between glucose concentration and the FL intensity. The photocatalytic studies showed that composite with high surface to volume ratio exhibits a maximum degradation of MB over 93 %. Our combined results ensured that the ZnO:rgo composites with varying morphologies could be an effective system for applications such as FL-based glucose sensing and photocatalytic degradation.

The Relationship between FL Reading Strategies and FL Reading Proficiency: A Study on Turkish EFL Learners

ERIC Educational Resources Information Center

Gönen, Ipek Kuru

2015-01-01

Reading in FL possesses certain challenges for FL readers such as difficulty in inferring underlying messages in texts and dealing with unfamiliar cultural load. All these challenges may be associated with FL learners' reading proficiency and their use of FL reading strategies especially while reading academic materials. This study aims at…

Assumptions about footprint layer heights influence the quantification of emission sources: a case study for Cyprus

NASA Astrophysics Data System (ADS)

Hüser, Imke; Harder, Hartwig; Heil, Angelika; Kaiser, Johannes W.

2017-09-01

Lagrangian particle dispersion models (LPDMs) in backward mode are widely used to quantify the impact of transboundary pollution on downwind sites. Most LPDM applications count particles with a technique that introduces a so-called footprint layer (FL) with constant height, in which passing air tracer particles are assumed to be affected by surface emissions. The mixing layer dynamics are represented by the underlying meteorological model. This particle counting technique implicitly assumes that the atmosphere is well mixed in the FL. We have performed backward trajectory simulations with the FLEXPART model starting at Cyprus to calculate the sensitivity to emissions of upwind pollution sources. The emission sensitivity is used to quantify source contributions at the receptor and support the interpretation of ground measurements carried out during the CYPHEX campaign in July 2014. Here we analyse the effects of different constant and dynamic FL height assumptions. The results show that calculations with FL heights of 100 and 300 m yield similar but still discernible results. Comparison of calculations with FL heights constant at 300 m and dynamically following the planetary boundary layer (PBL) height exhibits systematic differences, with daytime and night-time sensitivity differences compensating for each other. The differences at daytime when a well-mixed PBL can be assumed indicate that residual inaccuracies in the representation of the mixing layer dynamics in the trajectories may introduce errors in the impact assessment on downwind sites. Emissions from vegetation fires are mixed up by pyrogenic convection which is not represented in FLEXPART. Neglecting this convection may lead to severe over- or underestimations of the downwind smoke concentrations. Introducing an extreme fire source from a different year in our study period and using fire-observation-based plume heights as reference, we find an overestimation of more than 60  % by the constant FL height assumptions used for surface emissions. Assuming a FL that follows the PBL may reproduce the peak of the smoke plume passing through but erroneously elevates the background for shallow stable PBL heights. It might thus be a reasonable assumption for open biomass burning emissions wherever observation-based injection heights are not available.

Guidance, Control and Positioning of Future Precision Guided Stand-off Weapons Systems

DTIC Science & Technology

1986-06-01

environment tests. The programme consists of approximately ten fl ights, the firsts having a passive nature . These are followed by progressive...Limited _. .. , 40 Chigwell Lane, Loughton, Essex IGIO 3TZ PREFACE The environment in which tactical air forces must be able to operate is becoming...GUIDANCE SYSTEM CONCEPT FOR HIGH-DYNAMIC ENVIRONMENT * by U.K.Krogmann 17 APPLICATIONS DES CENTRALES A COMPOSANTS LIES AUX MISSILES TACTIQUES: CAS DES

Synchronization of Combat Power at the Task Force Level: Defining a Planning Methodology

DTIC Science & Technology

1989-01-01

tachments) LOCATION SCT ARTY CAS AH SMOKE MORTAR FASCAM TI ME EST ACTUAL. Current Future LD PL 1 X Atk Porn PL 2 PL3 OBJ X PL 1 LD PL 2 FL3 OBJ PL 2 PL 1...learned in medical school to 138 identify the aorta only to arrive at St. Elsewhere, where they call it "the big blue boy .* Right now the US Army has a

Evaluation of Alternatives for an Army Precision Landing System.

DTIC Science & Technology

1985-09-01

U FL - ! - Vv3- LN1.N V"VVu 𔄀V- - - UW Vr - V V 416 -60 ,.1 :0 4 - L DG6 -46. 0𔃺- Iii V34 0 c 0.- to C aIa a id4 C13 a ’hO. i 410 Id...USAAVNC CPT E. Veiga . Concepts and Studies Division CPT R. Wilson. Concepts Branch CPT R. Roberts. USAATCA Liaison CPT Flynn, Force Structures

Design Considerations for the ORION Satellite: Structure, Propulsion and Attitude Control Subsystems for a Small, General Purpose Spacecraft.

DTIC Science & Technology

1988-03-01

i~- .i-~-.-.-~’Y~..~w zmxmz- .,- PITN LI FIL &13EE AI BED O FL 11.8 GA O UHIGPSO 46.26 PISTON STROKE Fiur, 45 Experimental WAC CORPORAL Piston ... Tank (JPL TR 32-899, 1966, P. .3 361 c~’V spaced na’fles wnhch use the forces due to propellant fluid surface tension w position a portion of the liquid

U.S. Nuclear Weapons: Changes in Policy and Force Structure

DTIC Science & Technology

2008-01-23

Pinellas Plant , in Clearwater, FL; and the Pantex Plant near Amarillo, TX. These facilities were also operated by industrial contractors. Finally, the...These included the Rocky Flats Plant , outside Denver, CO; the Kansas City Plant , near Kansas City, MO; the Mound Plant , near Dayton OH; the...In 1988, DOE closed the nuclear reactors at Hanford and Savannah River, in response to safety concerns. The Rocky Flats Plant , which produced the

U.S. Nuclear Weapons: Changes in Policy and Force Structure

DTIC Science & Technology

2006-08-10

the Mound Plant , near Dayton OH; the Pinellas Plant , in Clearwater, FL; and the Pantex Plant near Amarillo, TX. These facilities were also operated...major nuclear weapons production facilities. These included the Rocky Flats Plant , outside Denver, CO; the Kansas City Plant , near Kansas City, MO...response to safety concerns. The Rocky Flats Plant , which produced the nuclear triggers, or “pits,” for nuclear weapons closed in 1989, in response

Fundamentals of Tribology Workshop Held at the University of Florida, Gainesville, Florida on 10-11 May 2010: Project Summary

DTIC Science & Technology

2010-05-01

to prevent fouling • Environmental Materials Water and hydrocarbons as third bodies Controllable friction (thermal, stress, chemical induced...Final Report Air Force Grant # FA9550-10-1-0261 Fundamentals of Tribology Workshop For the period: 03/17/2020-02/14/2011 Contract... Tribology An AFOSR Sponsored Workshop Gainesville, FL May 2010 Project Summary On May 10-11, 2010, leading scientists and engineers of the

Evaluation of the 29-km Eta Model. Part I: Objective Verification at Three Selected Stations

NASA Technical Reports Server (NTRS)

Manobianco, John; Nutter, Paul

1998-01-01

A subjective evaluation of the National Centers for Environmental Prediction 29-km (meso-) eta model during the 1996 warm (May-August) and cool (October-January) seasons is described. The overall evaluation assessed the utility of the model for operational weather forecasting by the U.S. Air Force 45th Weather Squadron, National Weather Service (NWS) Spaceflight Meteorology Group (SMG) and NWS Office in Melbourne, FL.

Dodecyl and octyl esters of fluorescein as protonophores and uncouplers of oxidative phosphorylation in mitochondria at submicromolar concentrations.

PubMed

Shchepinova, Maria M; Denisov, Stepan S; Kotova, Elena A; Khailova, Ljudmila S; Knorre, Dmitry A; Korshunova, Galina A; Tashlitsky, Vadim N; Severin, Fedor F; Antonenko, Yuri N

2014-01-01

In our search for fluorescent uncouplers of oxidative phosphorylation, three esters of fluorescein, n-butyl-, n-octyl-, and n-dodecyl-oxycarbonyl-fluorescein (C4-FL, C8-FL, C12-FL) were synthesized and characterized. With increasing liposomal lipid content, the long-chain alkyl derivatives of fluorescein (C8-FL, C12-FL and commercially available C18-FL), but not C4-FL and unsubstituted fluorescein, exhibited an increase in fluorescence polarization reflecting the dye binding to liposomes. C12-FL induced proton permeability in lipid membranes, while C4-FL was inactive. In contrast to C4-FL and C18-FL, C12-FL and C8-FL increased the respiration rate and decreased the membrane potential of isolated rat liver mitochondria with half-maximal effective concentrations of 700nM and 300nM, respectively. The effect of Cn-FL on the respiration correlated with that on proton permeability of the inner mitochondrial membrane, as measured by induction of mitochondria swelling in the potassium acetate medium. Binding of C8-FL to mitochondria depended on their energization, which was apparently associated with pH gradient generation across the inner mitochondrial membrane in the presence of a respiratory substrate. In wild-type yeast cells, C12-FL localized predominantly in plasma membrane, whereas in AD1-8 mutants lacking MDR pumps, it stained cytoplasmic organelles with some preference for mitochondria. Fluorescent uncouplers can be useful as a tool for determining their localization in a cell or distribution between different tissues in a living animal by fluorescent microscopy. © 2013.

Exploring the Origin of Blue and Ultraviolet Fluorescence in Graphene Oxide.

PubMed

Kozawa, Daichi; Miyauchi, Yuhei; Mouri, Shinichiro; Matsuda, Kazunari

2013-06-20

We studied the fluorescence (FL) properties of highly exfoliated graphene oxide (GO) in aqueous solution using continuous-wave and time-resolved FL spectroscopy. The FL spectra of highly exfoliated GO showed two distinct peaks at ∼440 (blue) and ∼300 nm [ultraviolet (UV)]. The FL of GO in the UV region at ∼300 nm was observed for the first time. The average FL lifetimes of the emission peaks at ∼440 and ∼300 nm are 8-13 and 6-8 ns, respectively. The experimentally observed peak wavelengths of pH-dependent FL, FL excitation spectra, and the FL lifetimes are nearly coincident with those of aromatic compounds bound with oxygen functional groups, which suggests that the FL comes from sp(2) fragments consisting of small numbers of aromatic rings with oxygen functional groups acting as FL centers in the GO.

Sensitive detection and estimation of cell-derived peroxynitrite fluxes using fluorescein-boronate.

PubMed

Rios, Natalia; Piacenza, Lucía; Trujillo, Madia; Martínez, Alejandra; Demicheli, Verónica; Prolo, Carolina; Álvarez, María Noel; López, Gloria V; Radi, Rafael

2016-12-01

The specific and sensitive detection of peroxynitrite (ONOO - /ONOOH) in biological systems is a great challenge due to its high reactivity towards several biomolecules. Herein, we validated the advantages of using fluorescein-boronate (Fl-B) as a highly sensitive fluorescent probe for the direct detection of peroxynitrite under biologically-relevant conditions in two different cell models. The synthesis of Fl-B was achieved by a very simply two-step conversion synthetic route with high purity (>99%) and overall yield (∼42%). Reactivity analysis of Fl-B with relevant biological oxidants including hydrogen peroxide (H 2 O 2 ), hypochlorous acid (HOCl) and peroxynitrite were performed. The rate constant for the reaction of peroxynitrite with Fl-B was 1.7×10 6 M -1 s -1 , a million times faster than the rate constant measured for H 2 O 2 (k=1.7M -1 s -1 ) and 2,700 faster than HOCl (6.2×10 2 M -1 s -1 ) at 37°C and pH 7.4. The reaction of Fl-B with peroxynitrite was significant even in the presence of physiological concentrations of CO 2 , a well-known peroxynitrite reactant. Experimental and simulated kinetic analyses confirm that the main oxidation process of Fl-B takes place with peroxynitrite itself via a direct bimolecular reaction and not with peroxynitrite-derived radicals. Fl-B was successfully applied for the detection of endogenously-generated peroxynitrite by endothelial cells and in macrophage-phagocyted parasites. Moreover, the generated data allowed estimating the actual intracellular flux of peroxynitrite. For instance, ionomycin-stimulated endothelial cells generated peroxynitrite at a rate of ∼ 0.1μMs -1 , while immunostimulated macrophages do so in the order of ∼1μMs -1 inside T. cruzi-infected phagosomes. Fl-B revealed not to be toxic in concentrations up to 1mM for 24h. Cellular peroxynitrite detection was achieved by conventional laboratory fluorescence-based methods including flow cytometry and epi-fluorescence microscopy. Fl-B was shown to be more sensitive than the coumarin boronate due to a higher molar absorption coefficient and quantum yield. Overall, our results show that Fl-B is a kinetically selective and highly sensitive probe for the direct detection of cell-derived peroxynitrite. Copyright © 2016 Elsevier Inc. All rights reserved.

Strategies of molecular imprinting-based fluorescence sensors for chemical and biological analysis.

PubMed

Yang, Qian; Li, Jinhua; Wang, Xiaoyan; Peng, Hailong; Xiong, Hua; Chen, Lingxin

2018-07-30

One pressing concern today is to construct sensors that can withstand various disturbances for highly selective and sensitive detecting trace analytes in complicated samples. Molecularly imprinted polymers (MIPs) with tailor-made binding sites are preferred to be recognition elements in sensors for effective targets detection, and fluorescence measurement assists in highly sensitive detection and user-friendly control. Accordingly, molecular imprinting-based fluorescence sensors (MI-FL sensors) have attracted great research interest in many fields such as chemical and biological analysis. Herein, we comprehensively review the recent advances in MI-FL sensors construction and applications, giving insights on sensing principles and signal transduction mechanisms, focusing on general construction strategies for intrinsically fluorescent or nonfluorescent analytes and improvement strategies in sensing performance, particularly in sensitivity. Construction strategies are well overviewed, mainly including the traditional indirect methods of competitive binding against pre-bound fluorescent indicators, employment of fluorescent functional monomers and embedding of fluorescence substances, and novel rational designs of hierarchical architecture (core-shell/hollow and mesoporous structures), post-imprinting modification, and ratiometric fluorescence detection. Furthermore, MI-FL sensor based microdevices are discussed, involving micromotors, test strips and microfluidics, which are more portable for rapid point-of-care detection and in-field diagnosing. Finally, the current challenges and future perspectives of MI-FL sensors are proposed. Copyright © 2018 Elsevier B.V. All rights reserved.

Study of plasmonics in hybrids made from a quantum emitter and double metallic nanoshell dimer

NASA Astrophysics Data System (ADS)

Guo, Jiaohan; Black, Kevin; Hu, Jiawen; Singh, Mahi

2018-05-01

We developed a theory for the fluorescence (FL) for quantum emitter and double metallic nanoshell dimer hybrids using the density matrix method. The dimer is made from two identical double metallic nanoshells, which are made of a dielectric core, a gold metallic shell and a dielectric spacer layer. The quantum emitters are deposited on the surface of the spacer layers of the dimers due to the electrostatic absorptions. We consider that dimer hybrids are surrounded by biological cells. This can be achieved by injecting them into human or animal cells. The surface plasmon polaritons (SPP) are calculated for the dimer using Maxwell’s equations in the static wave approximation. The calculated SPP energy agrees with experimental data from Zhai et al (2017 Plasmonics 12 263) for the dimer made from a silica core, a gold metallic nanoshell and a silica spacer layer. We have also obtained an analytical expression of the FL using the density matrix method. We compare our theory with FL experimental data from Zhai et al (2017 Plasmonics 12 263) where the FL spectrum was measured by varying the thickness of the spacer layer from 9 nm to 40 nm. A good agreement between theory and experiment is found. We have shown that the enhancement of the FL increases as the thickness of the spacer layer decreases. We have also found that the enhancement of the FL increases as the distance between the double metallic nanoshells in the dimer decreases. These are interesting findings which are consistent with the experiments of Zhai et al (2017 Plasmonics 12 263) and can be used to control the FL enhancement in the FL-based biomedical imaging and cancer treatment. These interesting findings may also be useful in the fabrication of nanosensors and nanoswitches for applications in medicine.

A Potential Approach for Low Flow Selection in Water Resource Supply and Management

Treesearch

Ying Ouyang

2012-01-01

Low flow selections are essential to water resource management, water supply planning, and watershed ecosystem restoration. In this study, a new approach, namely the frequent-low (FL) approach (or frequent-low index), was developed based on the minimum frequent-low flow or level used in minimum flows and/or levels program in northeast Florida, USA. This FL approach was...

Follicular Lymphoma in the United States: First Report of the National LymphoCare Study

PubMed Central

Friedberg, Jonathan W.; Taylor, Michael D.; Cerhan, James R.; Flowers, Christopher R.; Dillon, Hildy; Farber, Charles M.; Rogers, Eric S.; Hainsworth, John D.; Wong, Elaine K.; Vose, Julie M.; Zelenetz, Andrew D.; Link, Brian K.

2009-01-01

Purpose Optimal therapy of follicular lymphoma (FL) is not defined. We analyzed a large prospective cohort study to identify current demographics and patterns of care of FL in the United States. Patients and Methods The National LymphoCare Study is a multicenter, longitudinal, observational study designed to collect information on treatment regimens and outcomes for patients with newly diagnosed FL in the United States. Patients were enrolled between 2004 and 2007. There is no study-specific prescribed treatment regimen or intervention. Results Two thousand seven hundred twenty-eight subjects were enrolled at 265 sites, including the 80% of patients enrolled from nonacademic sites. Using the Follicular Lymphoma International Prognostic Index (FLIPI), three distinct groups independent of histologic grade could be defined. Initial therapeutic strategy was: observation, 17.7%; rituximab monotherapy, 13.9%; clinical trial 6.1%; radiation therapy, 5.6%; chemotherapy only, 3.2%; chemotherapy plus rituximab, 51.9%. Chemotherapy plus rituximab regimens were: rituximab plus cyclophosphamide, doxorubicin, vincristine, prednisone, 55.0%; rituximab plus cyclophosphamide, vincristine, and prednisone, 23.1%; rituximab plus fludarabine based, 15.5%; other, 6.4%. The choice to initiate therapy rather than observe was associated with age, FLIPI, stage, and grade (P < .01). Significant differences in treatment (P < .01) across regions of the United States were noted. Contrary to practice guidelines, treatment of stage I FL frequently omits radiation therapy. Conclusion Widely disparate therapeutic approaches are utilized for FL. Initial therapy is deferred in a small subset of patients. There is no single standard of care for the treatment of de novo FL, although antibody use is ubiquitous when therapy is initiated. These disparate approaches to the initial care of patients with FL render a heterogeneous group of patients at relapse. PMID:19204203

FAA Aviation Forecasts

DTIC Science & Technology

1992-02-01

MKK) Daytona Beach, FL (DAB) Boise, ID (BOI) Fort Lauderdale, FL (FLL) Idaho Falls Fanning Field, ID (IDA) Fort Lauderdale Executive, FL (FXE... Lewiston , ID (LWS) Fort Myers Page Field, FL (FMY) Pocatello, ID (PIH) Fort Myers Regional, FL (RSW) Twin Falls, ID (TWF) Fort Pierce, FL (FPR) Alton St... Idaho (SUN) 6. Marion Williamson County, Illinois (MWA) 7. Waukegan, Illinois (UGN) 8. Topeka-Phillip Ballard, Kansas (TOP) 9. Owensboro-Daviees County

MnSOD deficiency results in elevated oxidative stress and decreased mitochondrial function but does not lead to muscle atrophy during aging.

PubMed

Lustgarten, Michael S; Jang, Youngmok C; Liu, Yuhong; Qi, Wenbo; Qin, Yuejuan; Dahia, Patricia L; Shi, Yun; Bhattacharya, Arunabh; Muller, Florian L; Shimizu, Takahiko; Shirasawa, Takuji; Richardson, Arlan; Van Remmen, Holly

2011-06-01

In a previous study, we reported that a deficiency in MnSOD activity (approximately 80% reduction) targeted to type IIB skeletal muscle fibers was sufficient to elevate oxidative stress and to reduce muscle function in young adult mice (TnIFastCreSod2(fl/fl) mice). In this study, we used TnIFastCreSod2(fl/fl) mice to examine the effect of elevated oxidative stress on mitochondrial function and to test the hypothesis that elevated oxidative stress and decreased mitochondrial function over the lifespan of the TnIFastCreSod2(fl/fl) mice would be sufficient to accelerate muscle atrophy associated with aging. We found that mitochondrial function is reduced in both young and old TnIFastCreSod2(fl/fl) mice, when compared with control mice. Complex II activity is reduced by 47% in young and by approximately 90% in old TnIFastCreSod2(fl/fl) mice, and was found to be associated with reduced levels of the catalytic subunits for complex II, SDHA and SDHB. Complex II-linked mitochondrial respiration is reduced by approximately 70% in young TnIFastCreSod2(fl/fl) mice. Complex II-linked mitochondrial Adenosine-Tri-Phosphate (ATP) production is reduced by 39% in young and was found to be almost completely absent in old TnIFastCreSod2(fl/fl) mice. Furthermore, in old TnIFastCreSod2(fl/fl) mice, aconitase activity is almost completely abolished; mitochondrial superoxide release remains > 2-fold elevated; and oxidative damage (measured as F(2) - isoprostanes) is increased by 30% relative to age-matched controls. These data show that despite elevated skeletal muscle-specific mitochondrial oxidative stress, oxidative damage, and complex II-linked mitochondrial dysfunction, age-related muscle atrophy was not accelerated in old TnIFastCreSod2(fl/fl) mice, suggesting mitochondrial oxidative stress may not be causal for age-related muscle atrophy. No claim to original US government works. Aging Cell © 2011 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland.

Differential Gene Dosage Effects of Diabetes-Associated Gene GLIS3 in Pancreatic β Cell Differentiation and Function

PubMed Central

Bush, Sean P.; Wen, Xianjie; Cao, Wei; Chan, Lawrence

2017-01-01

Mutations of GLI-similar 3 (GLIS3) underlie a neonatal diabetes syndrome. Genome-wide association studies revealed that GLIS3 variants are associated with both common type 1 and type 2 diabetes. Global Glis3-deficient (Glis3−/−) mice die of severe diabetes shortly after birth. GLIS3 controls islet differentiation by transactivating neurogenin 3 (Ngn3). To unravel the function of Glis3 in adults, we generated inducible global Glis3-deficient mice (Glis3fl/fl/RosaCreERT2). Tamoxifen (TAM)-treated Glis3fl/fl/RosaCreERT2 mice developed severe diabetes, which was reproduced in TAM-treated β cell–specific Glis3fl/fl/Pdx1CreERT mice, but not in TAM-treated Glis3fl/fl/MipCreERT mice. Furthermore, we generated constitutive β cell– or pancreas-specific Glis3-deficient mice using either RipCre (Glis3fl/fl/RipCre) or Pdx1Cre (Glis3fl/fl/Pdx1Cre) coexpressing mice. We observed that, remarkably, neither type of β cell– or pancreas-specific Glis3-deficient mice phenocopied the lethal neonatal diabetes observed in Glis3−/− mice. All Glis3fl/fl/RipCre mice survived to adulthood with normal glucose tolerance. Thirty percent of Glis3fl/fl/Pdx1Cre mice developed severe diabetes at 3 to 4 weeks of age, whereas 55% of them developed mild diabetes with age. In contrast to the >90% reduction of Ngn3 and near-total absence of insulin (Ins) in the embryonic pancreas of Glis3−/− mice, we found only 75%–80% reduction of Ngn3 and Ins messenger RNA or protein expression in the fetal pancreas of Glis3fl/fl/Pdx1Cre mice. The expression levels of Ngn3 and Ins correlated negatively with the extent of Cre-mediated Glis3 deletion. These mouse models are powerful tools to decipher Glis3 gene dosage effects and the role of GLIS3 mutations/variants in a spectrum of β cell dysfunction in people. PMID:27813676

Publications - GMC 304 | Alaska Division of Geological & Geophysical

Science.gov Websites

DGGS GMC 304 Publication Details Title: Hard-rock geochemical data of core from the FL-001, FL-003, and . Bibliographic Reference Unknown, 2002, Hard-rock geochemical data of core from the FL-001, FL-003, and FL-004

CD10 down expression in follicular lymphoma correlates with gastrointestinal lesion involving the stomach and large intestine.

PubMed

Ohnishi, Nobuhiko; Takata, Katsuyoshi; Miyata-Takata, Tomoko; Sato, Yasuharu; Tari, Akira; Gion, Yuka; Noujima-Harada, Mai; Taniguchi, Kohei; Tabata, Tetsuya; Nagakita, Keina; Omote, Shizuma; Takahata, Hiroyuki; Iwamuro, Masaya; Okada, Hiroyuki; Maeda, Yoshinobu; Yanai, Hiroyuki; Yoshino, Tadashi

2016-11-01

Follicular lymphoma (FL) shows co-expression of B-cell lymphoma 2 (BCL2) and CD10, whereas downexpression of CD10 is occasionally experienced in gastrointestinal (GI) FL with unknown significance. Gastrointestinal FL is a rare variant of FL, and its similarity with mucosa-associated lymphoid tissue lymphoma was reported. We investigated the clinicopathological and genetic features of CD10 downexpressed (CD10 down ) GI-FL. The diagnosis of CD10 down FL was carried out with a combination of pathological and molecular analyses. The incidence of CD10 down GI-FL was shown in 35/172 (20.3%) cases, which was more frequent than nodal FL (3.5%, P < 0.001). The difference was additionally significant between GI-FL and nodal FL when the analysis was confined to primary GI-FL (55.2% vs 3.5%, P < 0.001). Compared to CD10 + GI-FL, CD10 down GI-FL significantly involved the stomach or large intestine (P = 0.015), and additionally showed the downexpression of BCL6 (P < 0.001). The follicular dendritic cell meshwork often showed a duodenal pattern in the CD10 down group (P = 0.12). Furthermore, a lymphoepithelial lesion was observed in 5/12 (40%) gastric FL cases, which indicated caution in the differentiation of mucosa-associated lymphoid tissue lymphoma. Molecular analyses were undertaken in seven cases of CD10 down GI-FL, and an identical clone was found between CD10 down follicles and CD10 + BCL2 + neoplastic follicles. In the diagnosis of cases with CD10 down BCL2 + follicles, careful examination with molecular studies should be carried out. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Inversion of surface parameters using fast learning neural networks

NASA Technical Reports Server (NTRS)

Dawson, M. S.; Olvera, J.; Fung, A. K.; Manry, M. T.

1992-01-01

A neural network approach to the inversion of surface scattering parameters is presented. Simulated data sets based on a surface scattering model are used so that the data may be viewed as taken from a completely known randomly rough surface. The fast learning (FL) neural network and a multilayer perceptron (MLP) trained with backpropagation learning (BP network) are tested on the simulated backscattering data. The RMS error of training the FL network is found to be less than one half the error of the BP network while requiring one to two orders of magnitude less CPU time. When applied to inversion of parameters from a statistically rough surface, the FL method is successful at recovering the surface permittivity, the surface correlation length, and the RMS surface height in less time and with less error than the BP network. Further applications of the FL neural network to the inversion of parameters from backscatter measurements of an inhomogeneous layer above a half space are shown.

Towards Resilient Critical Infrastructures: Application of Type-2 Fuzzy Logic in Embedded Network Security Cyber Sensor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ondrej Linda; Todd Vollmer; Jim Alves-Foss

2011-08-01

Resiliency and cyber security of modern critical infrastructures is becoming increasingly important with the growing number of threats in the cyber-environment. This paper proposes an extension to a previously developed fuzzy logic based anomaly detection network security cyber sensor via incorporating Type-2 Fuzzy Logic (T2 FL). In general, fuzzy logic provides a framework for system modeling in linguistic form capable of coping with imprecise and vague meanings of words. T2 FL is an extension of Type-1 FL which proved to be successful in modeling and minimizing the effects of various kinds of dynamic uncertainties. In this paper, T2 FL providesmore » a basis for robust anomaly detection and cyber security state awareness. In addition, the proposed algorithm was specifically developed to comply with the constrained computational requirements of low-cost embedded network security cyber sensors. The performance of the system was evaluated on a set of network data recorded from an experimental cyber-security test-bed.« less

46 CFR 7.95 - St. Johns Point, FL to Miami Beach, FL.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false St. Johns Point, FL to Miami Beach, FL. 7.95 Section 7... LINES Atlantic Coast § 7.95 St. Johns Point, FL to Miami Beach, FL. (a) A line drawn from the seaward extremity of St. Augustine Inlet north jetty to latitude 29°55′ N. longitude 81°15.3′ W. (St. Augustine...

Robust Fuzzy Controllers Using FPGAs

NASA Technical Reports Server (NTRS)

Monroe, Author Gene S., Jr.

2007-01-01

Electro-mechanical device controllers typically come in one of three forms, proportional (P), Proportional Derivative (PD), and Proportional Integral Derivative (PID). Two methods of control are discussed in this paper; they are (1) the classical technique that requires an in-depth mathematical use of poles and zeros, and (2) the fuzzy logic (FL) technique that is similar to the way humans think and make decisions. FL controllers are used in multiple industries; examples include control engineering, computer vision, pattern recognition, statistics, and data analysis. Presented is a study on the development of a PD motor controller written in very high speed hardware description language (VHDL), and implemented in FL. Four distinct abstractions compose the FL controller, they are the fuzzifier, the rule-base, the fuzzy inference system (FIS), and the defuzzifier. FL is similar to, but different from, Boolean logic; where the output value may be equal to 0 or 1, but it could also be equal to any decimal value between them. This controller is unique because of its VHDL implementation, which uses integer mathematics. To compensate for VHDL's inability to synthesis floating point numbers, a scale factor equal to 10(sup (N/4) is utilized; where N is equal to data word size. The scaling factor shifts the decimal digits to the left of the decimal point for increased precision. PD controllers are ideal for use with servo motors, where position control is effective. This paper discusses control methods for motion-base platforms where a constant velocity equivalent to a spectral resolution of 0.25 cm(exp -1) is required; however, the control capability of this controller extends to various other platforms.

A randomized clinical trial of theory-based activities for the behavioral symptoms of dementia in nursing home residents.

PubMed

Kolanowski, Ann; Litaker, Mark; Buettner, Lin; Moeller, Joyel; Costa, Paul T

2011-06-01

To test the main and interactive effects of activities derived from the Need-Driven Dementia-Compromised Behavior model for responding to behavioral symptoms in nursing home residents. Randomized double-blind clinical trial. Nine community-based nursing homes. One hundred twenty-eight cognitively impaired residents randomly assigned to activities adjusted to functional level (FL) (n=32), personality style of interest (PSI) (n=33), functional level and personality style of interest (FL+PSI) (n=31), or active control (AC) (n=32). Three weeks of activities provided twice daily. Agitation, passivity, engagement, affect, and mood assessed from video recordings and real-time observations during baseline, intervention, random times outside of intervention, and 1 week after intervention. All treatments improved outcomes during intervention except mood, which worsened under AC. During intervention the PSI group demonstrated greater engagement, alertness, and attention than the other groups; the FL+PSI group demonstrated greater pleasure. During random times, engagement returned to baseline levels except in the FL group in which it decreased. There was also less agitation and passivity in groups with a component adjusted to PSI. One week after the intervention, mood, anxiety, and passivity improved over baseline; significantly less pleasure was displayed after withdrawal of treatment. The hypothesis that activities adjusted to FL+PSI would improve behavioral outcomes to a greater extent than partially adjusted or nonadjusted activities was partially supported. PSI is a critical component of individualized activity prescription. © 2011, Copyright the Authors. Journal compilation © 2011, The American Geriatrics Society.

Modeling Item Responses When Different Subjects Employ Different Solution Strategies.

DTIC Science & Technology

1987-10-01

Crombag Dr. Stephen Dunbar University of Leyden Lindquist Center Education Research l-enter for Measurement Boerhaavelaan 2 University of Iowa 2334 EN... Leyden Iowa City, IA 52242 The NEFHlPLANDS Dr. James A. F.arles Mr. Iimothy Davey Air Force Human Resources Lab iJniversity of Illinois Brooks AFB, TX...Education and Training Dr. William Montague Naval Air Station NPRDC Code 13 Pensacola, fL 32508 San Diego, CA 92152-6800 Dr. Gary Marco Ms. Kathleen Moreno

A Leadership Competency Model for U.S. Air Force Wing Chaplains

DTIC Science & Technology

2009-02-12

45th Annual Conference of the International Military Testing Association, 3-6 November 2003, Pensacola, FL, 721-33, http://www.interrnationalmta.org...2005). 19 31 David C. McClelland, “ Testing for Competence Rather than for ‘Intelligence,’” American Psychologist 28, no. 1 (1973): 1-14. 32 C.K...Ronald Ash, Mariana Batista, Linda Carr, Lorraine D. Eyde , Beryl Hesketh, Jerry Kehoe, Kenneth Pearlman, Erich Prien, and Juan I. Sanchez, “The Practice of

Educating Special Forces Junior Leaders for a Complex Security Environment

DTIC Science & Technology

2009-07-01

Security Environment 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR( S ) 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK...UNIT NUMBER 7. PERFORMING ORGANIZATION NAME( S ) AND ADDRESS(ES) Joint Special Operations University,357 Tully Street Alison Building,Hurlburt Field,FL...32544 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING/MONITORING AGENCY NAME( S ) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S ACRONYM( S ) 11. SPONSOR

Evolution of the Florida Launch Site Architecture: Embracing Multiple Customers, Enhancing Launch Opportunities

NASA Technical Reports Server (NTRS)

Colloredo, Scott; Gray, James A.

2011-01-01

The impending conclusion of the Space Shuttle Program and the Constellation Program cancellation unveiled in the FY2011 President's budget created a large void for human spaceflight capability and specifically launch activity from the Florida launch Site (FlS). This void created an opportunity to re-architect the launch site to be more accommodating to the future NASA heavy lift and commercial space industry. The goal is to evolve the heritage capabilities into a more affordable and flexible launch complex. This case study will discuss the FlS architecture evolution from the trade studies to select primary launch site locations for future customers, to improving infrastructure; promoting environmental remediation/compliance; improving offline processing, manufacturing, & recovery; developing range interface and control services with the US Air Force, and developing modernization efforts for the launch Pad, Vehicle Assembly Building, Mobile launcher, and supporting infrastructure. The architecture studies will steer how to best invest limited modernization funding from initiatives like the 21 st elSe and other potential funding.

Similarities of prosurvival signals in Bcl-2-positive and Bcl-2-negative follicular lymphomas identified by reverse phase protein microarray.

PubMed

Zha, Hongbin; Raffeld, Mark; Charboneau, Lu; Pittaluga, Stefania; Kwak, Larry W; Petricoin, Emanuel; Liotta, Lance A; Jaffe, Elaine S

2004-02-01

Overexpression of Bcl-2 protein has been known to play a role in the pathogenesis of follicular lymphoma (FL). However, 10-15% of FLs are negative for Bcl-2 by immunohistochemistry, raising the possibility that another gene product(s) may provide prosurvival signal(s). We used reverse phase protein microarray to analyze lysates of follicle center cells isolated by laser capture microdissection from: Bcl-2+ FL, Bcl-2- FL and reactive follicular hyperplasia (FH) (nine cases each group). TUNEL assay confirmed similar and reduced levels of apoptosis in Bcl-2+ FL and Bcl-2- FL, indicating the likelihood of Bcl-2-independent inhibition of apoptosis. Arrays were quantitatively analyzed with antibodies to proteins involved in the apoptotic pathway. As expected, Bcl-2 levels were up to eight-fold higher in Bcl-2+ FL than in FH and Bcl-2- FL. However, there was no difference in levels of Mcl-1 and survivin among these three groups. Bcl-X(L) showed a trend for increased expression in Bcl-2- FL as compared with Bcl-2+ FL, although the differences did not reach statistical significance (P>0.1). The increase in Bcl-X(L) may provide an alternative antiapoptotic signal in FL negative for Bcl-2 protein. Interestingly, Bax expression was higher in FL (Bcl-2+ or -) than in FH (P=0.001). Notably, phospho-Akt (Ser-473) was increased in FL (Bcl-2+ or -) (P<0.03) with increased phospho-Bad (Ser-136), as compared with levels in FH. The activation of the Akt/Bad pathway provides further evidence of prosurvival signals in FL, independent of Bcl-2 alone. These data suggest that nodal FL represents a single disease with a final common biochemical pathway.

Fractionalized Fermi liquids and exotic superconductivity in the Kitaev-Kondo lattice

NASA Astrophysics Data System (ADS)

Seifert, Urban F. P.; Meng, Tobias; Vojta, Matthias

2018-02-01

Fractionalized Fermi liquids (FL*) have been introduced as non-Fermi-liquid metallic phases, characterized by coexisting electron-like charge carriers and local moments which form a fractionalized spin liquid. Here we investigate a Kondo lattice model on the honeycomb lattice with Kitaev interactions among the local moments, a concrete model hosting FL* phases based on Kitaev's Z2 spin liquid. We characterize the FL* phases via perturbation theory, and we employ a Majorana-fermion mean-field theory to map out the full phase diagram. Most remarkably we find nematic triplet superconducting phases which mask the quantum phase transition between fractionalized and conventional Fermi liquid phases. Their pairing structure is inherited from the Kitaev spin liquid; i.e., superconductivity is driven by Majorana glue.

Colpocephaly

MedlinePlus

... Organizations Birth Defect Research for Children, Inc. 976 Lake Baldwin Lane Suite 104 Orlando FL Orlando, FL ... Organizations Birth Defect Research for Children, Inc. 976 Lake Baldwin Lane Suite 104 Orlando FL Orlando, FL ...

33 CFR 80.805 - Rock Island, FL to Cape San Blas, FL.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Rock Island, FL to Cape San Blas, FL. 80.805 Section 80.805 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.805 Rock Island, FL...

33 CFR 80.805 - Rock Island, FL to Cape San Blas, FL.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Rock Island, FL to Cape San Blas, FL. 80.805 Section 80.805 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.805 Rock Island, FL...

33 CFR 80.805 - Rock Island, FL to Cape San Blas, FL.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Rock Island, FL to Cape San Blas, FL. 80.805 Section 80.805 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.805 Rock Island, FL...

33 CFR 80.805 - Rock Island, FL to Cape San Blas, FL.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Rock Island, FL to Cape San Blas, FL. 80.805 Section 80.805 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.805 Rock Island, FL...

33 CFR 80.805 - Rock Island, FL to Cape San Blas, FL.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Rock Island, FL to Cape San Blas, FL. 80.805 Section 80.805 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Eighth District § 80.805 Rock Island, FL...

33 CFR 80.753 - St. Petersburg, FL to the Anclote, FL.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false St. Petersburg, FL to the Anclote, FL. 80.753 Section 80.753 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Gulf Coast § 80.753 St. Petersburg, FL to...

33 CFR 80.750 - Sanibel Island, FL to St. Petersburg, FL.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Sanibel Island, FL to St. Petersburg, FL. 80.750 Section 80.750 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... St. Petersburg, FL. (a) A line formed by the centerline of the highway bridge over Blind Pass...

Longitudinal change of selected human milk oligosaccharides and association to infants' growth, an observatory, single center, longitudinal cohort study.

PubMed

Sprenger, Norbert; Lee, Le Ye; De Castro, Carlos Antonio; Steenhout, Philippe; Thakkar, Sagar K

2017-01-01

Human milk is the recommended and sole nutrient source for newborns. One of the largest components of human milk is oligosaccharides (HMOs) with major constituents determined by the mother genotype for the fucosyltransferase 2 (FUT2, secretor) gene. HMO variation has been related with infant microbiota establishment, diarrhea incidence, morbidity and mortality, IgE associated eczema and body composition. We investigated the (i) dependence of several major representative HMOs on the FUT2 status assessed through breast milk 2'Fucosyllactose (2'FL) and (ii) the relation of the 2'FL status with infant growth up to 4 months of life. From an open observatory, single center, longitudinal cohort study with quantitative human milk collection at 30, 60, and 120 days postpartum from 50 mothers, who gave birth to 25 female and 25 male singleton infants, we collected a representative sample of human milk. We quantified the following 5 representative HMOs: 2'FL, Lacto-N-tetraose (LNT), Lacto-N-neotetraose (LNnT), 3'Sialyllactose (3'SL) and 6'Sialyllactose (6'SL). We grouped the milk samples and corresponding infants according to the measured milk 2'FL concentrations at 30 days of lactation, which clustered around low concentrations (95% CI of mean 12-42 mg/L) and high concentrations (95% CI of mean 1880-2460 mg/L) with the former likely representing Secretor negative mothers. Infant anthropometric measures were recorded at birth, 1, 2 and 4 months of age. Relations among the quantified HMOs and the relation of the high and low 2'FL HMOs groups with infant growth parameters were investigated via linear mixed models. The milk samples with low 2'FL concentration had higher LNT and lower LNnT concentrations compared to the samples with high 2'FL. The milk 3'- and 6'SL concentrations were independent of 2'FL. Over lactation time we observed a drop in the concentration of 2'FL, LNT, LNnT and 6'SL, especially from 1 to 2 months, while 3'SL remained at relatively constant concentration from 1 month onwards. Up to 4 months of age, we did not observe significant differences in body weight, body length, body mass index and head circumference of the infants who consumed breast milk with low or high FUT2 associated HMO concentrations and composition. Our findings on HMO concentrations over time of lactation and clusters based on 2'FL concentrations confirm previous observations and suggest that LNnT and LNT are 'co-regulated' with the FUT2 dependent 2'FL concentration, with LNnT showing a positive and LNT a negative relation. Further, our findings also suggest that the relatively substantial variation in HMOs between the high and low 2'FL clusters do not impact infant growth of either sex up to 4 months of age. The study was registered in www.ClinicalTrial.gov (NCT01805011).

Conditional deletion of caspase-8 in macrophages alters macrophage activation in a RIPK-dependent manner.

PubMed

Cuda, Carla M; Misharin, Alexander V; Khare, Sonal; Saber, Rana; Tsai, FuNien; Archer, Amy M; Homan, Philip J; Haines, G Kenneth; Hutcheson, Jack; Dorfleutner, Andrea; Budinger, G R Scott; Stehlik, Christian; Perlman, Harris

2015-10-16

Although caspase-8 is a well-established initiator of apoptosis and suppressor of necroptosis, recent evidence suggests that this enzyme maintains functions beyond its role in cell death. As cells of the innate immune system, and in particular macrophages, are now at the forefront of autoimmune disease pathogenesis, we examined the potential involvement of caspase-8 within this population. Cre (LysM) Casp8 (fl/fl) mice were bred via a cross between Casp8 (fl/fl) mice and Cre (LysM) mice, and RIPK3 (-/-) Cre (LysM) Casp8 (fl/fl) mice were generated to assess the contribution of receptor-interacting serine-threonine kinase (RIPK)3. Immunohistochemical and immunofluorescence analyses were used to examine renal damage. Flow cytometric analysis was employed to characterize splenocyte distribution and activation. Cre (LysM) Casp8 (fl/fl) mice were treated with either Toll-like receptor (TLR) agonists or oral antibiotics to assess their response to TLR activation or TLR agonist removal. Luminex-based assays and enzyme-linked immunosorbent assays were used to measure cytokine/chemokine and immunoglobulin levels in serum and cytokine levels in cell culture studies. In vitro cell culture was used to assess macrophage response to cell death stimuli, TLR activation, and M1/M2 polarization. Data were compared using the Mann-Whitney U test. Loss of caspase-8 expression in macrophages promotes onset of a mild systemic inflammatory disease, which is preventable by the deletion of RIPK3. In vitro cell culture studies reveal that caspase-8-deficient macrophages are prone to a caspase-independent death in response to death receptor ligation; yet, caspase-8-deficient macrophages are not predisposed to unchecked survival, as analysis of mixed bone marrow chimeric mice demonstrates that caspase-8 deficiency does not confer preferential expansion of myeloid populations. Loss of caspase-8 in macrophages dictates the response to TLR activation, as injection of TLR ligands upregulates expression of costimulatory CD86 on the Ly6C(high)CD11b(+)F4/80(+) splenic cells, and oral antibiotic treatment to remove microbiota prevents splenomegaly and lymphadenopathy in Cre (LysM) Casp8 (fl/fl) mice. Further, caspase-8-deficient macrophages are hyperresponsive to TLR activation and exhibit aberrant M1 macrophage polarization due to RIPK activity. These data demonstrate that caspase-8 functions uniquely in macrophages by controlling the response to TLR activation and macrophage polarization in an RIPK-dependent manner.

Financial Literacy among Israeli College Students

ERIC Educational Resources Information Center

Shahrabani, Shosh

2013-01-01

In this study, responses of 574 students from two colleges in Israel were used to examine three issues: (a) financial literacy (FL) among Israeli college students, (b) gaps in FL between Jews and Arabs, and (c) factors affecting students' FL. The results showed that Israeli students exhibit a low level of FL and that FL is affected by gender,…

Respiration and carbon dynamics of free-living and particle-attached bacteria in coastal waters of NE Pacific

NASA Astrophysics Data System (ADS)

Guo, C.; Ke, Y.; Liu, H.

2016-02-01

Bacterial respiration (BR) rates are fundamental to understand the role of bacteria in carbon flow in aquatic ecosystem, and therefore it is critical to obtain reliable measurements. Prefiltration- (mostly 1-3μm) and dark-incubation- (mostly 24 h) based direct measurements of oxygen consumption have been the most commonly used method for BR. However, the prefiltration procedure and long incubation time may cause change of the bacterial abundance and structure, leading to inaccurate measurements. In this study, by measuring bacterial abundance, production and respiration of both particle-attached (PA) and free-living (FL) bacteria at two contrasting site in coastal NE Pacific from Nov 2014 to Mar 2015, we found that the 24 h growth rate of FL bacteria in the traditional BR incubations were significantly higher for 30% and 54% than those obtained for FL and total (FL+PA) bacteria in unfiltered incubations respectively, suggesting removal of protist grazers could cause a significant biomass accumulation during 24 h incubation than the in situ condition. This biomass overestimation resulted in 40% (±12%) overestimation of measured FL BR rates compared with the corrected in situ FL BR. Nevertheless, for the corrected in situ total BR, the rates were overestimated by traditional method in three measurements over nine for 6-46%, and were underestimated in the rest six measurements for 7-67%. Interestingly, those underestimations were attributed to the ignorance of PA bacteria due to prefiltration, which had larger cell size than the FL bacteria, accounted for 19% (±16%) in total bacterial abundance, and contributed to 50% (±19%) of total bacterial production. The average bacterial growth efficiency calculated by comparable 24 h integrated bacterial production and respiration was 0.42 (±0.24). Our results confirmed two major flaws in the current BR methodology, i.e., 1) it only measures the respiration of FL bacteria, and 2) the removal of grazers causing dramatic increase of bacteria abundance during the 24 h incubation, and attempted to provide a solution for correction. Although the problems causes magnificent overestimate of the respiration of the FL bacteria, it could either overestimate or underestimate the total BR due to the proportion of PA bacteria that was not measured in the standard BR methodology.

Findings and Implications of the Assignment Incentive Survey

DTIC Science & Technology

2002-10-01

Submarine Base Kings Bay is in Georgia. 27 In comparison, the Rotating to Sea sample (see figure 7) included Florida and Georgia and did not include...Because Kings Bay is a large submarine establishment, the Hawaii sample may have also been heavily in favor of FL/GA; however, the Hawaii sample was...Kings Bay , FL was listed as the most desirable fleet locadon for homebasing, fol- lowed by Bremerton/Bangor, Everett/Whidbey Island, Gulfport/Pas

Unusual Evolution of the Conduction-Electron State in CexLa1-xB6 from Non-Fermi Liquid to Fermi Liquid

NASA Astrophysics Data System (ADS)

Nakamura, S.; Endo, M.; Yamamoto, H.; Isshiki, T.; Kimura, N.; Aoki, H.; Nojima, T.; Otani, S.; Kunii, S.

2006-12-01

We report unusual evolution of the conduction-electron state in the localized f electron system CexLa1-xB6 from normal electron state to heavy Fermi liquid (FL) state through local FL and non-FL states with increasing Ce concentration and/or with increasing magnetic field. The effective mass of quasiparticle or the coefficient A of T2 term of resistivity is found to increase divergently near the boundary between FL state and non-FL state. The features of the non-FL state are also different from those of the typical non-FL systems previously observed or theoretically predicted.

The Seminole Serpent Warrior At Miramar, FL, Shows Settlement Locations Enabled Environmental Monitoring Reminiscent Of the Four-corners Kokopelli-like EMF Phenomena, and Related to Earthquakes, Tornados and Hurricanes.

NASA Astrophysics Data System (ADS)

Balam Matagamon, Chan; Pawa Matagamon, Sagamo

2004-03-01

Certain Native Americans of the past seem to have correctly deduced that significant survival information for their tradition-respecting cultures resided in EMF-based phenomena that they were monitoring. This is based upon their myths and the place or cult-hero names they bequeathed us. The sites we have located in FL have been detectable by us visually, usually by faint blue light, or by the elicitation of pin-like prickings, by somewhat intense nervous-system response, by EMF interactions with aural electrochemical systems that can elicit tinitus, and other ways. In the northeast, Cautantowit served as a harbinger of Indian summer, and appears to be another alter ego of the EMF. The Miami, FL Tequesta site along the river clearly correlates with tornado, earthquake and hurricane locations. Sites like the Mohave Deserts giant man may have had similar significance.

Mechanochromic behavior of a luminescent silicone rubber under tensile deformation

NASA Astrophysics Data System (ADS)

Kim, Yeon Ju; Lee, Sang Hwan; Jeong, Kwang-Un; Nah, Changwoon

2016-09-01

A novel mechanochromic elastomer based on silicone rubber and coumarin 6 dye have been prepared with various concentrations of the dye ranges from 2wt.% to a maximum of 5wt.% by solution mixing technique. After evaporating the solvent, cured samples were prepared as thin films using compression molding at 170° C. The optimum composition of the dye in rubber composites was determined based on the mechanochromic performance characterized with ultraviolet/visible (UV/Vis) spectrometer, x-ray diffraction (XRD) and spectrofluorometer (FL). The UV/Vis spectrometer monitors the dye aggregation in polymer film during the tensile deformation. The XRD monitors the change in size of dye aggregates. The FL monitors the optical response during tensile deformation due to the re-arrangement of dyes. As increasing a mechanical deformation to the polymeric composite film, UV/Vis absorption intensity was decreased and the FL emission wavelength was moved to decrease wavelength because of breaking dye aggregations. Also, XRD intensity peak was decreased, which dye aggregations were broken after mechanical deformation.

Flavagline analog FL3 induces cell cycle arrest in urothelial carcinoma cell of the bladder by inhibiting the Akt/PHB interaction to activate the GADD45α pathway.

PubMed

Yuan, Gangjun; Chen, Xin; Liu, Zhuowei; Wei, Wensu; Shu, Qinghai; Abou-Hamdan, Hussein; Jiang, Lijuan; Li, Xiangdong; Chen, Rixin; Désaubry, Laurent; Zhou, Fangjian; Xie, Dan

2018-02-07

Prohibitin 1 (PHB) is a potential target for the treatment of urothelial carcinoma of the bladder (UCB). FL3 is a newly synthesized agent that inhibits cancer cell proliferation by targeting the PHB protein; however, the effect of FL3 in UCB cells remains unexplored. FL3 was identified to be a potent inhibitor of UCB cell viability using CCK-8 (cell counting kit-8) assay. Then a series of in vitro and in vivo experiments were conducted to further demonstrate the inhibitory effect of FL3 on UCB cell proliferation and to determine the underlying mechanisms. FL3 inhibited UCB cell proliferation and growth both in vitro and in vivo. By targeting the PHB protein, FL3 inhibited the interaction of Akt and PHB as well as Akt-mediated PHB phosphorylation, which consequently decreases the localization of PHB in the mitochondria. In addition, FL3 treatment resulted in cell cycle arrest in the G2/M phase, and this inhibitory effect of FL3 could be mimicked by knockdown of PHB. Through the microarray analysis of mRNA expression after FL3 treatment and knockdown of PHB, we found that the mRNA expression of the growth arrest and DNA damage-inducible alpha (GADD45α) gene were significantly upregulated. When knocked down the expression of GADD45α, the inhibitory effect of FL3 on cell cycle was rescued, suggesting that FL3-induced cell cycle inhibition is GADD45α dependent. Our data provide that FL3 inhibits the interaction of Akt and PHB, which in turn activates the GADD45α-dependent cell cycle inhibition in the G2/M phase.

Lipophilic and hydrophilic antioxidant capacities of common foods in the United States.

PubMed

Wu, Xianli; Beecher, Gary R; Holden, Joanne M; Haytowitz, David B; Gebhardt, Susan E; Prior, Ronald L

2004-06-16

Both lipophilic and hydrophilic antioxidant capacities were determined using the oxygen radical absorbance capacity (ORAC(FL)) assay with fluorescein as the fluorescent probe and 2,2'-azobis(2-amidinopropane) dihydrochloride as a peroxyl radical generator on over 100 different kinds of foods, including fruits, vegetables, nuts, dried fruits, spices, cereals, infant, and other foods. Most of the foods were collected from four different regions and during two different seasons in U.S. markets. Total phenolics of each sample were also measured using the Folin-Ciocalteu reagent. Hydrophilic ORAC(FL) values (H-ORAC(FL)) ranged from 0.87 to 2641 micromol of Trolox equivalents (TE)/g among all of the foods, whereas lipophilic ORAC(FL) values (L-ORAC(FL)) ranged from 0.07 to 1611 micromol of TE/g. Generally, L-ORAC(FL) values were <10% of the H-ORAC(FL) values except for a very few samples. Total antioxidant capacity was calculated by combining L-ORAC(FL) and H-ORAC(FL). Differences of ORAC(FL) values in fruits and vegetables from different seasons and regions were relatively large for some foods but could not be analyzed in detail because of the sampling scheme. Two different processing methods, cooking and peeling, were used on selected foods to evaluate the impact of processing on ORAC(FL). The data demonstrated that processing can have significant effects on ORAC(FL). Considering all of the foods analyzed, the relationship between TP and H-ORAC(FL) showed a very weak correlation. Total hydrophilic and lipophilic antioxidant capacity intakes were calculated to be 5558 and 166 micromol of TE/day, respectively, on the basis of data from the USDA Continuing Survey of Food Intakes by Individuals (1994-1996).

Mu-opioid receptors in nociceptive afferents produce a sustained suppression of hyperalgesia in chronic pain.

PubMed

Severino, Amie; Chen, Wenling; Hakimian, Joshua K; Kieffer, Brigitte L; Gaveriaux-Ruff, Claire; Walwyn, Wendy; Marvizon, Juan Carlos

2018-04-17

The latent sensitization model of chronic pain reveals that recovery from some types of long-term hyperalgesia is an altered state in which nociceptive sensitization persists but is suppressed by the ongoing activity of analgesic receptors such as µ-opioid receptors (MORs). To determine whether these MORs are the ones present in nociceptive afferents, we bred mice expressing Cre-recombinase under the Nav1.8 channel promoter (Nav1.8cre) with MOR-floxed mice (flMOR). These Nav1.8cre/flMOR mice had reduced MOR expression in primary afferents, as revealed by quantitative PCR, in situ hybridization and immunofluorescence colocalization with the neuropeptide CGRP. We then studied the recovery from chronic pain of these mice and their flMOR littermates. When Nav1.8cre/flMOR mice were injected in the paw with complete Freund's adjuvant they developed mechanical hyperalgesia that persisted for over two months, whereas the responses of flMOR mice returned to baseline after three weeks. We then used the inverse agonist naltrexone to assess ongoing MOR activity. Naltrexone produced a robust reinstatement of hyperalgesia in control flMOR mice, but produced no effect in the Nav1.8/flMOR males and a weak reinstatement of hyperalgesia in Nav1.8/flMOR females. Naltrexone also reinstated swelling of the hind paw in flMOR mice and female Nav1.8cre/flMOR mice, but not male Nav1.8cre/flMOR mice. The MOR agonist DAMGO inhibited substance P release in flMOR mice but not Nav1.8cre/flMOR mice, demonstrating a loss of MOR function at the central terminals of primary afferents. We conclude that MORs in nociceptive afferents mediate an ongoing suppression of hyperalgesia to produce remission from chronic pain.

Air Force construction automation/robotics

NASA Technical Reports Server (NTRS)

Nease, A. D.; Alexander, E. F.

1993-01-01

The Air Force has several missions which generate unique requirements that are being met through the development of construction robotic technology. One especially important mission will be the conduct of Department of Defense (DOD) space activities. Space operations and other missions place construction/repair equipment operators in dangerous environments and potentially harmful situations. Additionally, force reductions require that human resources be leveraged to the maximum extent possible, and more stringent construction repair requirements push for increased automation. To solve these problems, the U.S. Air Force is undertaking a research and development effort at Tyndall AFB, FL, to develop robotic construction/repair equipment. This development effort involves the following technologies: teleoperation, telerobotics, construction operations (excavation, grading, leveling, tool change), robotic vehicle communications, vehicle navigation, mission/vehicle task control architecture, and associated computing environment. The ultimate goal is the fielding of a robotic repair capability operating at the level of supervised autonomy. This paper will discuss current and planned efforts in space construction/repair, explosive ordnance disposal, hazardous waste cleanup, and fire fighting.

MET Signaling Mediates Intestinal Crypt-Villus Development, Regeneration, and Adenoma Formation and Is Promoted by Stem Cell CD44 Isoforms.

PubMed

Joosten, Sander P J; Zeilstra, Jurrit; van Andel, Harmen; Mijnals, R Clinton; Zaunbrecher, Joost; Duivenvoorden, Annet A M; van de Wetering, Marc; Clevers, Hans; Spaargaren, Marcel; Pals, Steven T

2017-10-01

Resistance of metastatic human colorectal cancer cells to drugs that block epidermal growth factor (EGF) receptor signaling could be caused by aberrant activity of other receptor tyrosine kinases, activating overlapping signaling pathways. One of these receptor tyrosine kinases could be MET, the receptor for hepatocyte growth factor (HGF). We investigated how MET signaling, and its interaction with CD44 (a putative MET coreceptor regulated by Wnt signaling and highly expressed by intestinal stem cells [ISCs] and adenomas) affects intestinal homeostasis, regeneration, and adenoma formation in mini-gut organoids and mice. We established organoid cultures from ISCs stimulated with HGF or EGF and assessed intestinal differentiation by immunohistochemistry. Mice with total epithelial disruption of MET (Ah Cre /Met fl/fl /LacZ) or ISC-specific disruption of MET (Lgr5 Creert2 /Met fl/fl /LacZ) and control mice (Ah Cre /Met +/+ /LacZ, Lgr5 Creert2 /Met +/+ /LacZ) were exposed to 10 Gy total body irradiation; intestinal tissues were collected, and homeostasis and regeneration were assessed by immunohistochemistry. We investigated adenoma organoid expansion stimulated by HGF or EGF using adenomas derived from Lgr5 Creert2 /Met fl/fl /Apc fl/fl and Lgr5 Creert2 /Met +/+ /Apc fl/fl mice. The same mice were evaluated for adenoma prevalence and size. We also quantified adenomas in Ah Cre /Met fl/fl /Apc fl/+ mice compared with Ah Cre /Met +/+ /Apc fl/+ control mice. We studied expansion of organoids generated from crypts and adenomas, stimulated by HGF or EGF, that were derived from mice expressing different CD44 splice variants (Cd44 +/+ , Cd44 -/- , Cd44 s/s , or Cd44 v4-10/v4-10 mice). Crypts incubated with EGF or HGF expanded into self-organizing mini-guts with similar levels of efficacy and contained all differentiated cell lineages. MET-deficient mice did not have defects in intestinal homeostasis. Total body irradiation reduced numbers of proliferating crypts in Ah Cre /Met fl/fl /LacZ mice. Lgr5 Creert2 /Met fl/fl /LacZ mice had impaired regeneration of MET-deficient ISCs. Adenoma organoids stimulated with EGF or HGF expanded to almost twice the size of nonstimulated organoids. MET-deficient adenoma organoids did not respond to HGF stimulation, but did respond to EGF. ISC-specific disruption of Met (Lgr5 Creert2 /Met fl/fl /Apc fl/fl mice) caused a twofold increase in apoptosis in microadenomas, resulting in an approximately 50% reduction of microadenoma numbers and significantly reduced average adenoma size. Total epithelial disruption of Met (Ah Cre /Met fl/fl /Apc fl/+ mice) resulted in an approximate 50% reduction in (micro)adenoma numbers. Intestinal crypts from Cd44 -/- mice did not expand to the same extent as crypts from Cd44 +/+ mice on stimulation with HGF, but had the same response to EGF. The negative effect on HGF-mediated growth was overcome by expression of CD44v4-10, but not by CD44s. Similarly, HGF-mediated expansion of adenoma organoids required CD44v4-10. In studies of intestinal organoid cultures and mice with inducible deletion of MET, we found HGF receptor signaling to regulate intestinal homeostasis and regeneration, as well as adenoma formation. These activities of MET are promoted by the stem cell CD44 isoform CD44v4-10. Our findings provide rationale for targeting signaling via MET and CD44 during anti-EGF receptor therapy of patients with colorectal cancer or in patients resistant to EGF receptor inhibitors. Copyright © 2017 AGA Institute. Published by Elsevier Inc. All rights reserved.

Mathematical Techniques for Nonlinear System Theory.

DTIC Science & Technology

1979-05-01

7 7 AD—A078 715 FLORIDA UNIV GAINESVILLE CENTER FOR MATHEMATICAL SYS——ETC FIG 12/1 MATHEMATICAL TECHNIQUES FOR NONLINEAR SYSTEM THEORY . (U) MAY 79... System Theory / 61102F ~~~~~ ~ ~~~~~~~~ Gainesville , FL 32601 L ~~~ CONTROLLING OFFI C E NAME A N D ADDRES S . Air Force Office of Scientific... System Theory During the past year, the major effort under this grant was work by the Principal Investigator (R. E. Kalman) and by E. Emre

Fuze Experimentation Facility and Fuze Industrial Facility (FEF/FIF) Construction. Final Environmental Assessment

DTIC Science & Technology

2011-04-01

1-9. R esources N ot C arried Forw ard for D etailed A nalysis Legend Environmental Justice Concerns c:::J Proposed Project Area No Concerns...11 FEF/FIF C onstruction Environm ental A ssessm ent Page 3-6 Eglin A ir Force B ase, FL Final Figure 3-1. W ater R esources A t or N...Ecological A ssociations and Biological R esources A t or N ear the Proposed A ction Location Ecological Association Flatwoods Landscaped!Urban c:J

Analyzing the Operational Factors of Space, Force, and Time in United States Southern Command’s Counter Drug / Counter Narcoterrorism Mission

DTIC Science & Technology

2010-05-03

countries by the President. Of the 20 nations listed as meeting the criteria for 2008, 12 are found in SOUTHCOM’s AOR: The Bahamas, Bolivia , Brazil...Venezuela and Bolivia ), making them harder to catch on their way to Mexico or, increasingly, overseas.” 26 (Italics added for emphasis) Beyond pure...Southern Command. Command Strategy 2018 . Miami, FL: December, 2008. ------. “Counter Drug / Counter Narcoterrorism.” www.southcom.mil/AppsSC/pages

Investigation of Pseudo Bi-Polar Nickel Cadmium Batteries as Filter Elements for Pulsed Power Loads.

DTIC Science & Technology

1984-12-01

FOR PULSED POWER LOADS THESIS Michael B. Cimino Gregory M. Gearing Major, USAF Captain, USAF AFIT/GE/ENG/84D-1B DTIC SECETE D~rR~fl"N STATEMENT A...LOADS THESIS Presented to the Faculty of the School of Engineering of the Air Force Institute of Technology Air University In Partial Fulfillment of...with the intent to make batteries capable of out performing capacitors as power supply filters. Purpose This thesis investigated the use of nickel

Magnetic Field, Force, and Inductance Computations for an Axially Symmetric Solenoid

NASA Technical Reports Server (NTRS)

Lane, John E.; Youngquist, Robert C.; Immer, Christopher D.; Simpson, James C.

2001-01-01

The pumping of liquid oxygen (LOX) by magnetic fields (B field), using an array of electromagnets, is a current topic of research and development at Kennedy Space Center, FL. Oxygen is paramagnetic so that LOX, like a ferrofluid, can be forced in the direction of a B field gradient. It is well known that liquid oxygen has a sufficient magnetic susceptibility that a strong magnetic gradient can lift it in the earth's gravitational field. It has been proposed that this phenomenon can be utilized in transporting (i.e., pumping) LOX not only on earth, but on Mars and in the weightlessness of space. In order to design and evaluate such a magnetic pumping system, it is essential to compute the magnetic and force fields, as well as inductance, of various types of electromagnets (solenoids). In this application, it is assumed that the solenoids are air wrapped, and that the current is essentially time independent.

Fluorescence-labeled methylation-sensitive amplified fragment length polymorphism (FL-MS-AFLP) analysis for quantitative determination of DNA methylation and demethylation status.

PubMed

Kageyama, Shinji; Shinmura, Kazuya; Yamamoto, Hiroko; Goto, Masanori; Suzuki, Koichi; Tanioka, Fumihiko; Tsuneyoshi, Toshihiro; Sugimura, Haruhiko

2008-04-01

The PCR-based DNA fingerprinting method called the methylation-sensitive amplified fragment length polymorphism (MS-AFLP) analysis is used for genome-wide scanning of methylation status. In this study, we developed a method of fluorescence-labeled MS-AFLP (FL-MS-AFLP) analysis by applying a fluorescence-labeled primer and fluorescence-detecting electrophoresis apparatus to the existing method of MS-AFLP analysis. The FL-MS-AFLP analysis enables quantitative evaluation of more than 350 random CpG loci per run. It was shown to allow evaluation of the differences in methylation level of blood DNA of gastric cancer patients and evaluation of hypermethylation and hypomethylation in DNA from gastric cancer tissue in comparison with adjacent non-cancerous tissue.

Influence of extraction solvent on antioxidant capacity value of oleaster measured by ORAC method.

PubMed

Yalcin, Gorkem; Sogut, Ozlem

2014-01-01

Oxygen radical absorbance capacity (ORAC) is a widely used hydrogen atom transfer-based method which measures the antioxidant capacity of natural products. ORAC values of oleaster (Elaeagnus angustifolia L.), which was extracted with ethanol/acetone (7:3, v/v), ethanol/water (1:1, v/v) and methanol/water (1:1, v/v) in order to evaluate the effects of solvent type on antioxidant capacity, were examined. In general, results revealed that ethanol/water extracts exhibited better antioxidant capacity values. Furthermore, results obtained by using ORAC-eosin y (ORAC-EY), one of the widely used derivative of fluorescein (FL), as a fluorescent probe were compared with those obtained by using ORAC-FL. According to the results, ORAC-EY values were found to be compatible with ORAC-FL values.

The disulfide isomerase ERp57 is required for fibrin deposition in vivo.

PubMed

Zhou, J; Wu, Y; Wang, L; Rauova, L; Hayes, V M; Poncz, M; Essex, D W

2014-11-01

ERp57 is required for platelet function; however, whether ERp57 contributes to fibrin generation is unknown. Using an inhibitory anti-ERp57 antibody (mAb1), Pf4-Cre/ERp57(fl/fl) mice, Tie2-Cre/ERp57(fl/fl) mice, and mutants of ERp57, we analyzed the function of ERp57 in laser-induced thrombosis. Fibrin deposition was decreased in Pf4-Cre/ERp57(fl/fl) mice, consistent with a role for platelet ERp57 in fibrin generation. Fibrin deposition was further decreased with infusion of mAb1 and in Tie2-Cre/ERp57(fl/fl) mice, consistent with endothelial cells also contributing to fibrin deposition. Infusion of eptibifatide inhibited platelet and fibrin deposition, confirming a role for platelets in fibrin deposition. Infusion of recombinant ERp57 corrected the defect in fibrin deposition but not platelet accumulation, suggesting a direct effect of ERp57 on coagulation. mAb1 inhibited thrombin generation in vitro, consistent with a requirement for ERp57 in coagulation. Platelet accumulation was decreased to similar extents in Pf4-Cre/ERp57(fl/fl) mice, Tie2-Cre/ERp57(fl/fl) mice and normal mice infused with mAb1. Infusion of completely inactivated ERp57 or ERp57 with a non-functional second active site inhibited fibrin deposition and platelet accumulation, indicating that the isomerase activity of the second active site is required for these processes. ERp57 regulates thrombosis via multiple targets. © 2014 International Society on Thrombosis and Haemostasis.

Comparison of clinicopathologic characteristics of gastric follicular lymphomas and duodenal follicular lymphomas.

PubMed

Maeshima, Akiko Miyagi; Taniguchi, Hirokazu; Suzuki, Tomotaka; Yuda, Sayako; Toyoda, Kosuke; Yamauchi, Nobuhiko; Makita, Shinichi; Fukuhara, Suguru; Munakata, Wataru; Maruyama, Dai; Kobayashi, Yukio; Saito, Yutaka; Tobinai, Kensei

2017-07-01

We compared the incidence, esophagogastroduodenoscopy (EGD) findings, and histopathologic characteristics of gastric and duodenal follicular lymphomas (FL). Of 626 FL cases, primary gastric FL and secondary gastric involvement of FL were observed in 1% and 5% of the cases, respectively, which were lower incidences than duodenal FL (10% and 9%, respectively). Gastric FL usually appeared as submucosal tumors (primary, 71%; secondary, 79%), whereas duodenal FL, as granular lesions (primary, 92%: secondary, 87%). In the granular duodenal lesions, the neoplastic follicles were located sparsely on the muscularis mucosa and could be found between villi, whereas in the stomach, similar lesions were hidden within the lamina propria, and only larger lesions such as submucosal tumors could be detected on the mucosal surface. The differences in the incidences and EGD findings were considered to be associated with structural differences of the lamina propria. Typical FL features: grades 1-2 histology, follicularity, and CD10 + and/or BCL6 + and BCL2 + were usually observed in all primary and secondary gastric and duodenal FL. Gastroduodenal and bone marrow involvement were found in 12% and 33% of the cases, respectively, and there was no significant correlation between them (P=.095). Twenty-nine cases (5%) were up-staged by gastroduodenal-positive results. In conclusion, the histopathology of gastric FL was similar to that of duodenal and nodal FL; the differences in the incidence and EGD findings between gastric and duodenal FL were considered to be associated with structural difference of the lamina propria, and EGD was useful as a staging procedure. Copyright © 2017 Elsevier Inc. All rights reserved.

Thermodynamic and electron paramagnetic resonance characterization of flavin in succinate dehydrogenase.

PubMed

Ohnishi, T; King, T E; Salerno, J C; Blum, H; Bowyer, J R; Maida, T

1981-06-10

Thermodynamic parameters of succinate dehydrogenase flavin were determined potentiometrically from the analysis of free radical signal levels as a function of the oxidation-reduction potential. Midpoint redox potentials of consecutive 1-electron transfer steps are -127 and -31 mV at pH 7.0. This corresponds to a stability constant of intermediate stability, 2.5 x 10(-2), which suggests flavin itself may be a converter from n = 2 to n = 1 electron transfer steps. The pK values of the free radical (FlH . in equilibrium Fl . -) and the fully reduced form (FlH2 in equilibrium FlH-) were estimated as 8.0 +/- 0.2 and 7.7 +/- 0.2, respectively. Succinate dehydrogenase flavosemiquinone elicits an EPR spectrum at g = 2.00 with a peak to peak width of 1.2 mT even in the protonated form, suggesting the delocalization in the unpaired electron density. A close proximity of succinate dehydrogenase flavin and iron-sulfur cluster S-1 was demonstrated based on the enhancement of flavin spin relaxation by Center S-1.

Investigation of arterial bloodgases at altitude using constant-flow oxygen masks.

PubMed

Hodgson, W R; Wright, R C; Nelson, G C; Letchford, T

1978-06-01

Arterial blood oxygen tensions up to 6700 m altitude (FL220) were measured polarographically while subjects breathed from various masks with constant-flow oxygen. The Sierra and Aro masks used for emergency decompression descent in commercial passenger aircraft, gave mean PaO2's of 130 +/- 7.6 and 130 +/- 12.1 torr at 6700 m (FL220) and 90 +/- 3.8 and 77 +/- 3.35 torr at 4260 m (FL140), respectively, when supplied with oxygen flows corresponding to those available in the Boeing 747. These oxygen tensions during descent are acceptable for normal physiological function in a heterogeneous population of air travellers whereas breathing ambient air during return to base at 4260 m (FL 140) (PaO2 of 48 or less) is not acceptable. The valveless Hudson 1007 and Puritan 114011 masks, used for air ambulance service, gave mean PaO2's of 110 +/- 2.7 and 98 +/- 4.5 torr at 6700 m and 80 +/- 3.0 and 77 +/- 2.5 torr at 4260 m under the same condition--significantly less than the Sierra mask.

What's technology cooking up? A systematic review of the use of technology in adolescent food literacy programs.

PubMed

Wickham, Catherine A; Carbone, Elena T

2018-06-01

Over one-third of adolescents are overweight or obese. Food literacy (FL), the ability to plan and manage, select, prepare, and eat healthy foods, is a contemporary concept that provides a mechanism to understand the relationship between food-related knowledge and skills and dietary intake. Innovative interventions which focus on the core concepts of FL and include generationally appropriate technology have the potential to provide positive impact on the dietary habits of adolescents. This systematic review followed PRISMA guidelines and employed the Downs and Black criteria for rating studies. Titles and abstracts of 545 articles were collected and reviewed from 13 electronic databases. Studies were selected if they were peer-reviewed, included adolescents 12-19 years-old, incorporated concepts related to FL, and employed technology as part of the intervention. Eight studies, six randomized controlled trials (RCT) and two interventions without controls were included. Seven of the interventions used Internet or web-based platforms to access program components and all RCTs incorporated game elements. Studies included between two and four constructs of FL. All reported positive changes in food intake with five reporting significant positive pre- and post-intervention changes. Few technology-driven FL-related studies exist within the literature. Although all studies reported improvements in dietary intake, due to variation in program design, delivery, and evaluation it is difficult to tease out the effect of the technology component. Continued research is needed to: 1) determine the degree to which FL should be included in interventions to effect a positive change on dietary intake; 2) develop adolescent-specific FL measures to more appropriately evaluate changes in knowledge, food-related skills, and dietary intake; and 3) design technology-driven interventions so that technology components can be analyzed separately from other program elements. Copyright © 2018 Elsevier Ltd. All rights reserved.

Regulation of iNOS function and cellular redox state by macrophage Gch1 reveals specific requirements for tetrahydrobiopterin in NRF2 activation

PubMed Central

McNeill, Eileen; Crabtree, Mark J.; Sahgal, Natasha; Patel, Jyoti; Chuaiphichai, Surawee; Iqbal, Asif J.; Hale, Ashley B.; Greaves, David R.; Channon, Keith M.

2015-01-01

Inducible nitric oxide synthase (iNOS) is a key enzyme in the macrophage inflammatory response, which is the source of nitric oxide (NO) that is potently induced in response to proinflammatory stimuli. However, the specific role of NO production, as distinct from iNOS induction, in macrophage inflammatory responses remains unproven. We have generated a novel mouse model with conditional deletion of Gch1, encoding GTP cyclohydrolase 1 (GTPCH), an essential enzyme in the biosynthesis of tetrahydrobiopterin (BH4) that is a required cofactor for iNOS NO production. Mice with a floxed Gch1 allele (Gch1fl/fl) were crossed with Tie2cre transgenic mice, causing Gch1 deletion in leukocytes (Gch1fl/flTie2cre). Macrophages from Gch1fl/flTie2cre mice lacked GTPCH protein and de novo biopterin biosynthesis. When activated with LPS and IFNγ, macrophages from Gch1fl/flTie2cre mice induced iNOS protein in a manner indistinguishable from wild-type controls, but produced no detectable NO, as judged by L-citrulline production, EPR spin trapping of NO, and by nitrite accumulation. Incubation of Gch1fl/flTie2cre macrophages with dihydroethidium revealed significantly increased production of superoxide in the presence of iNOS expression, and an iNOS-independent, BH4-dependent increase in other ROS species. Normal BH4 levels, nitric oxide production, and cellular redox state were restored by sepiapterin, a precursor of BH4 production by the salvage pathway, demonstrating that the effects of BH4 deficiency were reversible. Gch1fl/flTie2cre macrophages showed only minor alterations in cytokine production and normal cell migration, and minimal changes in basal gene expression. However, gene expression analysis after iNOS induction identified 78 genes that were altered between wild-type and Gch1fl/flTie2cre macrophages. Pathway analysis identified decreased NRF2 activation, with reduced induction of archetypal NRF2 genes (gclm, prdx1, gsta3, nqo1, and catalase) in BH4-deficient Gch1fl/flTie2cre macrophages. These findings identify BH4-dependent iNOS regulation and NO generation as specific requirements for NRF2-dependent responses in macrophage inflammatory activation. PMID:25451639

Longitudinal change of selected human milk oligosaccharides and association to infants’ growth, an observatory, single center, longitudinal cohort study

PubMed Central

Lee, Le Ye; De Castro, Carlos Antonio; Steenhout, Philippe; Thakkar, Sagar K.

2017-01-01

Background Human milk is the recommended and sole nutrient source for newborns. One of the largest components of human milk is oligosaccharides (HMOs) with major constituents determined by the mother genotype for the fucosyltransferase 2 (FUT2, secretor) gene. HMO variation has been related with infant microbiota establishment, diarrhea incidence, morbidity and mortality, IgE associated eczema and body composition. Objectives We investigated the (i) dependence of several major representative HMOs on the FUT2 status assessed through breast milk 2’Fucosyllactose (2’FL) and (ii) the relation of the 2’FL status with infant growth up to 4 months of life. Design From an open observatory, single center, longitudinal cohort study with quantitative human milk collection at 30, 60, and 120 days postpartum from 50 mothers, who gave birth to 25 female and 25 male singleton infants, we collected a representative sample of human milk. We quantified the following 5 representative HMOs: 2’FL, Lacto-N-tetraose (LNT), Lacto-N-neotetraose (LNnT), 3’Sialyllactose (3’SL) and 6’Sialyllactose (6’SL). We grouped the milk samples and corresponding infants according to the measured milk 2’FL concentrations at 30 days of lactation, which clustered around low concentrations (95% CI of mean 12–42 mg/L) and high concentrations (95% CI of mean 1880–2460 mg/L) with the former likely representing Secretor negative mothers. Infant anthropometric measures were recorded at birth, 1, 2 and 4 months of age. Relations among the quantified HMOs and the relation of the high and low 2’FL HMOs groups with infant growth parameters were investigated via linear mixed models. Results The milk samples with low 2’FL concentration had higher LNT and lower LNnT concentrations compared to the samples with high 2’FL. The milk 3’- and 6’SL concentrations were independent of 2’FL. Over lactation time we observed a drop in the concentration of 2’FL, LNT, LNnT and 6’SL, especially from 1 to 2 months, while 3’SL remained at relatively constant concentration from 1 month onwards. Up to 4 months of age, we did not observe significant differences in body weight, body length, body mass index and head circumference of the infants who consumed breast milk with low or high FUT2 associated HMO concentrations and composition. Conclusions Our findings on HMO concentrations over time of lactation and clusters based on 2’FL concentrations confirm previous observations and suggest that LNnT and LNT are ‘co-regulated’ with the FUT2 dependent 2’FL concentration, with LNnT showing a positive and LNT a negative relation. Further, our findings also suggest that the relatively substantial variation in HMOs between the high and low 2’FL clusters do not impact infant growth of either sex up to 4 months of age. The study was registered in www.ClinicalTrial.gov (NCT01805011). PMID:28182762

Role of Klotho in Osteoporosis and Renal Osteodystrophy

DTIC Science & Technology

2015-10-01

uremia induced increases in FGF23 transcription (Figure 6). VEGFa Runx2 Osx Col1a1 ALP OC 0 2 4 6 8 KL fl/fl Prx1-Cre; KL fl/fl m R N A ex pr es...week old mice revealed that Prx1cre;Klothofl/fl mice have significantly higher expression of osteoblastic and osteocytic markers such as Col1a1 , Runx2

Fbxw7 Deletion Accelerates KrasG12D-Driven Pancreatic Tumorigenesis via Yap Accumulation.

PubMed

Zhang, Qiang; Zhang, Yaqing; Parsels, Joshua D; Lohse, Ines; Lawrence, Theodore S; Pasca di Magliano, Marina; Sun, Yi; Morgan, Meredith A

2016-11-01

Pancreatic cancers driven by KRAS mutations require additional mutations for tumor progression. The tumor suppressor FBXW7 is altered in pancreatic cancers, but its contribution to pancreatic tumorigenesis is unknown. To determine potential cooperation between Kras mutation and Fbxw7 inactivation in pancreatic tumorigenesis, we generated P48-Cre;LSL-Kras G12D ;Fbxw7 fl/fl (KFC fl/fl ) compound mice. We found that KFC fl/fl mice displayed accelerated tumorigenesis: all mice succumbed to pancreatic ductal adenocarcinoma (PDA) by 40 days of age, with PDA onset occurring by 2 weeks of age. PDA in KFC fl/fl mice was preceded by earlier onset of acinar-to-ductal metaplasia (ADM) and pancreatic intraepithelial neoplasia (PanIN) lesions, and associated with chromosomal instability and the accumulation of Fbxw7 substrates Yes-associated protein (Yap), c-Myc, and Notch. Using KFC fl/fl and FBXW7-deficient human pancreatic cancer cells, we found that Yap silencing attenuated growth promotion by Fbxw7 deletion. Our data demonstrate that Fbxw7 is a potent suppressor of Kras G12D -induced pancreatic tumorigenesis due, at least in part, to negative regulation of Yap. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

ANITA-2000 activation code package - updating of the decay data libraries and validation on the experimental data of the 14 MeV Frascati Neutron Generator

NASA Astrophysics Data System (ADS)

Frisoni, Manuela

2016-03-01

ANITA-2000 is a code package for the activation characterization of materials exposed to neutron irradiation released by ENEA to OECD-NEADB and ORNL-RSICC. The main component of the package is the activation code ANITA-4M that computes the radioactive inventory of a material exposed to neutron irradiation. The code requires the decay data library (file fl1) containing the quantities describing the decay properties of the unstable nuclides and the library (file fl2) containing the gamma ray spectra emitted by the radioactive nuclei. The fl1 and fl2 files of the ANITA-2000 code package, originally based on the evaluated nuclear data library FENDL/D-2.0, were recently updated on the basis of the JEFF-3.1.1 Radioactive Decay Data Library. This paper presents the results of the validation of the new fl1 decay data library through the comparison of the ANITA-4M calculated values with the measured electron and photon decay heats and activities of fusion material samples irradiated at the 14 MeV Frascati Neutron Generator (FNG) of the NEA-Frascati Research Centre. Twelve material samples were considered, namely: Mo, Cu, Hf, Mg, Ni, Cd, Sn, Re, Ti, W, Ag and Al. The ratios between calculated and experimental values (C/E) are shown and discussed in this paper.

Ablative fractional laser treatment for hypertrophic scars: comparison between Er:YAG and CO2 fractional lasers.

PubMed

Choi, Jae Eun; Oh, Ga Na; Kim, Jong Yeob; Seo, Soo Hong; Ahn, Hyo Hyun; Kye, Young Chul

2014-08-01

Nonablative fractional photothermolysis has been reported to show early promise in the treatment of hypertrophic scars, but there are few reports on ablative fractional photothermolysis for the treatment of hypertrophic scars. To evaluate and compare the efficacy and safety of Er:YAG fractional laser (EYFL) and CO2 fractional laser (CO2FL) for treatment of hypertrophic scars. Thirteen patients with hypertrophic scars were treated with 2,940 nm EYFL, and ten were treated with 10,600 nm CO2FL. An independent physician evaluator assessed the treatment outcomes using Vancouver scar scale (VSS) and 5-point grading scale (grade 0, no improvement; grade 1, 1-25%; grade 2, 26-50%; grade 3, 51-75%; grade 4, 76-100% improvement). Patients are queried about their subjective satisfaction with the treatment outcomes. After the final treatment, average percentage changes of VSS were 28.2% for EYFL and 49.8% for CO2FL. Improvement was evident in terms of pliability, while insignificant in terms of vascularity and pigmentation. Based on physician's global assessment, mean grade of 1.8 for EYFL and 2.7 for CO2FL was achieved. Patient's subjective satisfaction scores paralleled the physician's objective evaluation. CO2FL is a potentially effective and safe modality for the treatment of hypertrophic scars, particularly in terms of pliability.

The Maize Imprinted Gene Floury3 Encodes a PLATZ Protein Required for tRNA and 5S rRNA Transcription through Interaction with RNA Polymerase III[OPEN

PubMed Central

Wang, Jiechen; Ye, Jianwei; Zheng, Xixi; Xiang, Xiaoli; Li, Changsheng; Fu, Miaomiao; Wang, Qiong; Zhang, Zhiyong; Wu, Yongrui

2017-01-01

Maize (Zea mays) floury3 (fl3) is a classic semidominant negative mutant that exhibits severe defects in the endosperm but fl3 plants otherwise appear normal. We cloned the fl3 gene and determined that it encodes a PLATZ (plant AT-rich sequence and zinc binding) protein. The mutation in fl3 resulted in an Asn-to-His replacement in the conserved PLATZ domain, creating a dominant allele. Fl3 is specifically expressed in starchy endosperm cells and regulated by genomic imprinting, which leads to the suppressed expression of fl3 when transmitted through the male, perhaps as a consequence the semidominant behavior. Yeast two-hybrid screening and bimolecular luciferase complementation experiments revealed that FL3 interacts with the RNA polymerase III subunit 53 (RPC53) and transcription factor class C 1 (TFC1), two critical factors of the RNA polymerase III (RNAPIII) transcription complex. In the fl3 endosperm, the levels of many tRNAs and 5S rRNA that are transcribed by RNAPIII are significantly reduced, suggesting that the incorrectly folded fl3 protein may impair the function of RNAPIII. The transcriptome is dramatically altered in fl3 mutants, in which the downregulated genes are primarily enriched in pathways related to translation, ribosome, misfolded protein responses, and nutrient reservoir activity. Collectively, these changes may lead to defects in endosperm development and storage reserve filling in fl3 seeds. PMID:28874509

Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic)

NASA Astrophysics Data System (ADS)

Ortega-Retuerta, E.; Joux, F.; Jeffrey, W. H.; Ghiglione, J. F.

2013-04-01

We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis-single-strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a. Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7% similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, for the coast and river samples and both PA and FL fractions, Betaproteobacteria, Alphaproteobacteria and Actinobacteria were dominant. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a basis to predict further changes in bacterial communities should climate change lead to further increases in river discharge and related particle loads.

Reduced endothelial thioredoxin-interacting protein protects arteries from damage induced by metabolic stress in vivo.

PubMed

Bedarida, Tatiana; Domingues, Alison; Baron, Stephanie; Ferreira, Chrystophe; Vibert, Francoise; Cottart, Charles-Henry; Paul, Jean-Louis; Escriou, Virginie; Bigey, Pascal; Gaussem, Pascale; Leguillier, Teddy; Nivet-Antoine, Valerie

2018-06-01

Although thioredoxin-interacting protein (TXNIP) is involved in a variety of biologic functions, the contribution of endothelial TXNIP has not been well defined. To investigate the endothelial function of TXNIP, we generated a TXNIP knockout mouse on the Cdh5-cre background (TXNIP fl/fl cdh5 cre ). Control (TXNIP fl/fl ) and TXNIP fl/fl cdh5 cre mice were fed a high protein-low carbohydrate (HP-LC) diet for 3 mo to induce metabolic stress. We found that TXNIP fl/fl and TXNIP fl/fl cdh5 cre mice on an HP-LC diet displayed impaired glucose tolerance and dyslipidemia concretizing the metabolic stress induced. We evaluated the impact of this metabolic stress on mice with reduced endothelial TXNIP expression with regard to arterial structure and function. TXNIP fl/fl cdh5 cre mice on an HP-LC diet exhibited less endothelial dysfunction than littermate mice on an HP-LC diet. These mice were protected from decreased aortic medial cell content, impaired aortic distensibility, and increased plasminogen activator inhibitor 1 secretion. This protective effect came with lower oxidative stress and lower inflammation, with a reduced NLRP3 inflammasome expression, leading to a decrease in cleaved IL-1β. We also show the major role of TXNIP in inflammation with a knockdown model, using a TXNIP-specific, small interfering RNA included in a lipoplex. These findings demonstrate a key role for endothelial TXNIP in arterial impairments induced by metabolic stress, making endothelial TXNIP a potential therapeutic target.-Bedarida, T., Domingues, A., Baron, S., Ferreira, C., Vibert, F., Cottart, C.-H., Paul, J.-L., Escriou, V., Bigey, P., Gaussem, P., Leguillier, T., Nivet-Antoine, V. Reduced endothelial thioredoxin-interacting protein protects arteries from damage induced by metabolic stress in vivo.

Impaired clearance of influenza A virus in obese, leptin receptor deficient mice is independent of leptin signaling in the lung epithelium and macrophages.

PubMed

Radigan, Kathryn A; Morales-Nebreda, Luisa; Soberanes, Saul; Nicholson, Trevor; Nigdelioglu, Recep; Cho, Takugo; Chi, Monica; Hamanaka, Robert B; Misharin, Alexander V; Perlman, Harris; Budinger, G R Scott; Mutlu, Gökhan M

2014-01-01

During the recent H1N1 outbreak, obese patients had worsened lung injury and increased mortality. We used a murine model of influenza A pneumonia to test the hypothesis that leptin receptor deficiency might explain the enhanced mortality in obese patients. We infected wild-type, obese mice globally deficient in the leptin receptor (db/db) and non-obese mice with tissue specific deletion of the leptin receptor in the lung epithelium (SPC-Cre/LepR fl/fl) or macrophages and alveolar type II cells (LysM-Cre/Lepr fl/fl) with influenza A virus (A/WSN/33 [H1N1]) (500 and 1500 pfu/mouse) and measured mortality, viral clearance and several markers of lung injury severity. The clearance of influenza A virus from the lungs of mice was impaired in obese mice globally deficient in the leptin receptor (db/db) compared to normal weight wild-type mice. In contrast, non-obese, SP-C-Cre+/+/LepR fl/fl and LysM-Cre+/+/LepR fl/fl had improved viral clearance after influenza A infection. In obese mice, mortality was increased compared with wild-type mice, while the SP-C-Cre+/+/LepR fl/fl and LysM-Cre+/+/LepR fl/fl mice exhibited improved survival. Global loss of the leptin receptor results in reduced viral clearance and worse outcomes following influenza A infection. These findings are not the result of the loss of leptin signaling in lung epithelial cells or macrophages. Our results suggest that factors associated with obesity or with leptin signaling in non-myeloid populations such as natural killer and T cells may be associated with worsened outcomes following influenza A infection.

Association between plasma fluorescent oxidation products and erectile dysfunction: A prospective study.

PubMed

Yang, Shuman; Giovannucci, Edward; Bracken, Bruce; Ho, Shuk-Mei; Wu, Tianying

2015-08-14

Existing epidemiological studies of the association between oxidative stress and erectile dysfunction (ED) are sparse and inconclusive, which is likely due to cross-sectional design and small sample size. Therefore, we investigated the association between biomarkers of oxidative stress and ED in prospective setting among a relatively large sample size of men. We conducted the prospective study among 917 men ages between 47 and 80 years at the time of blood draw, which is a part of nested prospective case-control study of prostate cancer in the Health Professionals Follow-up Study. Plasma fluorescent oxidation products (FlOPs), a global biomarker for oxidative stress, were measured at three excitation/emission wavelengths (360/420 nm named as FlOP_360; 320/420 nm named as FlOP_320 and 400/475 nm named as FlOP_400). Approximately 35% of men developed ED during follow-up. We did not find an independent association between FlOP_360, FlOP_320, FlOP_400 and risk of ED in the multivariable adjusted model (Tertile 3 vs. tertile 1: odds ratio [OR] = 0.90, 95% confidence interval [CI] = 0.61-1.34, P(trend) = 0.54 for FlOP_360; OR = 0.73, 95% CI = 0.49-1.07, P(trend) = 0.27 for FlOP_320; and OR = 0.98, 95% CI = 0.66-1.45, P(trend) = 0.72 for FlOP_400). Further analysis of the association between FlOPs and ED in the fasting samples or controls only (free of prostate cancer incidence) did not change the results appreciably. Plasma FlOPs were not associated with the risk of ED, suggesting oxidative stress may not be an independent risk factor for ED.

Estimation of ligament strains and joint moments in the ankle during a supination sprain injury.

PubMed

Wei, Feng; Fong, Daniel Tik-Pui; Chan, Kai-Ming; Haut, Roger C

2015-01-01

This study presents the ankle ligament strains and ankle joint moments during an accidental injury event diagnosed as a grade I anterior talofibular ligament (ATaFL) sprain. A male athlete accidentally sprained his ankle while performing a cutting motion in a laboratory setting. The kinematic data were input to a three-dimensional rigid-body foot model for simulation analyses. Maximum strains in 20 ligaments were evaluated in simulations that investigated various combinations of the reported ankle joint motions. Temporal strains in the ATaFL and the calcaneofibular ligament (CaFL) were then compared and the three-dimensional ankle joint moments were evaluated from the model. The ATaFL and CaFL were highly strained when the inversion motion was simulated (10% for ATaFL and 12% for CaFL). These ligament strains were increased significantly when either or both plantarflexion and internal rotation motions were added in a temporal fashion (up to 20% for ATaFL and 16% for CaFL). Interestingly, at the time strain peaked in the ATaFL, the plantarflexion angle was not large but apparently important. This computational simulation study suggested that an inversion moment of approximately 23 N m plus an internal rotation moment of approximately 11 N m and a small plantarflexion moment may have generated a strain of 15-20% in the ATaFL to produce a grade I ligament injury in the athlete's ankle. This injury simulation study exhibited the potentially important roles of plantarflexion and internal rotation, when combined with a large inversion motion, to produce a grade I ATaFL injury in the ankle of this athlete.

Effects of FlAsH/Tetracysteine (TC) tag on PrP proteolysis and PrPres formation by TC-scanning

PubMed Central

Taguchi, Yuzuru; Hohsfield, Lindsay A.; Hollister, Jason R.

2014-01-01

The FlAsH/tetracysteine (FlAsH/TC) tag is a powerful tool for fluorescent labeling of proteins. However, even small tags such as FlAsH/TC could alter the behavior of the tagged proteins, especially if the insertion occurs at internal sites. Defining the influence of FlAsH/TC on nearby protein-protein interactions might aid in selecting appropriate positions for internal TC insertions and allow the exploitation of serial FlAsH/TC insertions (TC-scanning) as a probe to characterize sites of protein-protein interaction. To explore this application in the context of substrate-protease interactions, we analyzed the effect of FlAsH/TC insertions on proteolysis of cellular prion protein (PrPsen) in in vitro reactions and generation of the C1 metabolic fragment of PrPsen in live neuroblastoma cells. The influence of FlAsH/TC insertion was evaluated by TC-scanning across the cleavage sites of each protease. The results showed that FlAsH/TC inhibited protease cleavage only within limited ranges of the cleavage sites that varied from about 1 to 6 residues-wide depending on the protease, providing an estimate of the PrP residues interacting with each protease. TC-scanning was also used to probe a different type of protein-protein interaction, the conformational conversion of FlAsH-PrPsen to the prion disease-associated isoform, PrPres. PrP constructs with FlAsH/TC insertions at residues 90–96 but not 97–101 were converted to FlAsH-PrPres, identifying a boundary separating loosely versus compactly folded regions of PrPres. Our observations demonstrate that TC-scanning with the FlAsH/TC tag can be a versatile method for probing protein-protein interactions and folding processes. PMID:23943295

Fine mapping and candidate gene analysis of qFL-chr1, a fiber length QTL in cotton.

PubMed

Xu, Peng; Gao, Jin; Cao, Zhibin; Chee, Peng W; Guo, Qi; Xu, Zhenzhen; Paterson, Andrew H; Zhang, Xianggui; Shen, Xinlian

2017-06-01

A fiber length QTL, qFL-chr1, was fine mapped to a 0.9 cM interval of cotton chromosome 1. Two positional candidate genes showed positive correlation between gene expression level and fiber length. Prior analysis of a backcross-self mapping population derived from a cross between Gossypium hirsutum L. and G. barbadense L. revealed a QTL on chromosome 1 associated with increased fiber length (qFL-chr1), which was confirmed in three independent populations of near-isogenic introgression lines (NIILs). Here, a single NIIL, R01-40-08, was used to develop a large population segregating for the target region. Twenty-two PCR-based polymorphic markers used to genotype 1672 BC 4 F 2 plants identified 432 recombinants containing breakpoints in the target region. Substitution mapping using 141 informative recombinants narrowed the position of qFL-chr1 to a 1.0-cM interval between SSR markers MUSS084 and CIR018. To exclude possible effects of non-target introgressions on fiber length, different heterozygous BC 4 F 3 plants introgressed between SSR markers NAU3384 and CGR5144 were selected to develop sub-NILs. The qFL-chr1 was further mapped at 0.9-cM interval between MUSS422 and CIR018 by comparisons of sub-NIL phenotype, and increased fiber length by ~1 mm. The 2.38-Mb region between MUSS422 and CIR018 in G. barbadense contained 19 annotated genes. Expression levels of two of these genes, GOBAR07705 (encoding 1-aminocyclopropane-1-carboxylate synthase) and GOBAR25992 (encoding amino acid permease), were positively correlated with fiber length in a small F 2 population, supporting these genes as candidates for qFL-chr1.

Proteomic profiling of a mouse model of acute intestinal Apc deletion leads to identification of potential novel biomarkers of human colorectal cancer (CRC).

PubMed

Hammoudi, Abeer; Song, Fei; Reed, Karen R; Jenkins, Rosalind E; Meniel, Valerie S; Watson, Alastair J M; Pritchard, D Mark; Clarke, Alan R; Jenkins, John R

2013-10-25

Colorectal cancer (CRC) is the fourth most common cause of cancer-related death worldwide. Accurate non-invasive screening for CRC would greatly enhance a population's health. Adenomatous polyposis coli (Apc) gene mutations commonly occur in human colorectal adenomas and carcinomas, leading to Wnt signalling pathway activation. Acute conditional transgenic deletion of Apc in murine intestinal epithelium (AhCre(+)Apc(fl)(/)(fl)) causes phenotypic changes similar to those found during colorectal tumourigenesis. This study comprised a proteomic analysis of murine small intestinal epithelial cells following acute Apc deletion to identify proteins that show altered expression during human colorectal carcinogenesis, thus identifying proteins that may prove clinically useful as blood/serum biomarkers of colorectal neoplasia. Eighty-one proteins showed significantly increased expression following iTRAQ analysis, and validation of nine of these by Ingenuity Pathaway Analysis showed they could be detected in blood or serum. Expression was assessed in AhCre(+)Apc(fl)(/)(fl) small intestinal epithelium by immunohistochemistry, western blot and quantitative real-time PCR; increased nucelolin concentrations were also detected in the serum of AhCre(+)Apc(fl)(/)(fl) and Apc(Min)(/)(+) mice by ELISA. Six proteins; heat shock 60kDa protein 1, Nucleolin, Prohibitin, Cytokeratin 18, Ribosomal protein L6 and DEAD (Asp-Glu-Ala-Asp) box polypeptide 5,were selected for further investigation. Increased expression of 4 of these was confirmed in human CRC by qPCR. In conclusion, several novel candidate biomarkers have been identified from analysis of transgenic mice in which the Apc gene was deleted in the intestinal epithelium that also showed increased expression in human CRC. Some of these warrant further investigation as potential serum-based biomarkers of human CRC. Copyright © 2013 Elsevier Inc. All rights reserved.

Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity.

PubMed

Li, Ying; You, Qiang-Long; Zhang, Sheng-Rong; Huang, Wei-Yuan; Zou, Wen-Jun; Jie, Wei; Li, Shu-Ji; Liu, Ji-Hong; Lv, Chuang-Ye; Cong, Jin; Hu, Yu-Ying; Gao, Tian-Ming; Li, Jian-Ming

2017-04-18

Special AT-rich sequence-binding protein 2 (Satb2) is a protein binding to the matrix attachment regions of DNA and important for gene regulation. Patients with SATB2 mutation usually suffer moderate to severe mental retardation. However, the mechanisms for the defects of intellectual activities in patients with SATB2 mutation are largely unclear. Here we established the heterozygous Satb2 mutant mice and Satb2 conditional knockout mice to mimic the patients with SATB2 mutation and figured out the role of Satb2 in mental activities. We found that the spatial memory and working memory were significantly damaged in the heterozygous Satb2 mutant mice, early postnatal Satb2-deficient mice (CaMKIIα-Cre + Satb2 fl/fl mice), and adult Satb2 ablation mice (Satb2 fl/fl mice injected with CaMKIIα-Cre virus). Functionally, late phase long-term potentiation (L-LTP) in these Satb2 mutant mice was greatly impaired. Morphologically, in CA1 neurons of CaMKIIα-Cre + Satb2 fl/fl mice, we found decreased spine density of the basal dendrites and less branches of apical dendrites that extended into lacunar molecular layer. Mechanistically, expression levels of immediate early genes (IEGs) including Fos, FosB, and Egr1 were significantly decreased after Satb2 deletion. And, Satb2 could regulate expression of FosB by binding to the promoter of FosB directly. In general, our study uncovers that Satb2 plays an important role in spatial memory and working memory by regulating IEGs-mediated hippocampal synaptic plasticity.

Assessing niche separation among coexisting Limnohabitans strains through interactions with a competitor, viruses, and a bacterivore.

PubMed

Simek, Karel; Kasalický, Vojtech; Hornák, Karel; Hahn, Martin W; Weinbauer, Markus G

2010-03-01

We investigated potential niche separation in two closely related (99.1% 16S rRNA gene sequence similarity) syntopic bacterial strains affiliated with the R-BT065 cluster, which represents a subgroup of the genus Limnohabitans. The two strains, designated B4 and D5, were isolated concurrently from a freshwater reservoir. Differences between the strains were examined through monitoring interactions with a bacterial competitor, Flectobacillus sp. (FL), and virus- and predator-induced mortality. Batch-type cocultures, designated B4+FL and D5+FL, were initiated with a similar biomass ratio among the strains. The proportion of each cell type present in the cocultures was monitored based on clear differences in cell sizes. Following exponential growth for 28 h, the cocultures were amended by the addition of two different concentrations of live or heat-inactivated viruses concentrated from the reservoir. Half of virus-amended treatments were inoculated immediately with an axenic flagellate predator, Poterioochromonas sp. The presence of the predator, of live viruses, and of competition between the strains significantly affected their population dynamics in the experimentally manipulated treatments. While strains B4 and FL appeared vulnerable to environmental viruses, strain D5 did not. Predator-induced mortality had the greatest impact on FL, followed by that on D5 and then B4. The virus-vulnerable B4 strain had smaller cells and lower biomass yield, but it was less subject to grazing. In contrast, the seemingly virus-resistant D5, with slightly larger grazing-vulnerable cells, was competitive with FL. Overall, our data suggest contrasting ecophysiological capabilities and partial niche separation in two coexisting Limnohabitans strains.

Second Malignancy Risks After Non-Hodgkin's Lymphoma and Chronic Lymphocytic Leukemia: Differences by Lymphoma Subtype

PubMed Central

Morton, Lindsay M.; Curtis, Rochelle E.; Linet, Martha S.; Bluhm, Elizabeth C.; Tucker, Margaret A.; Caporaso, Neil; Ries, Lynn A.G.; Fraumeni, Joseph F.

2010-01-01

Purpose Previous studies have shown increased risks of second malignancies after non-Hodgkin's lymphoma (NHL) and chronic lymphocytic leukemia (CLL); however, no earlier investigation has quantified differences in risk of new malignancy by lymphoma subtype. Patients and Methods We evaluated second cancer and leukemia risks among 43,145 1-year survivors of CLL/small lymphocytic lymphoma (SLL), diffuse large B-cell lymphoma (DLBCL), or follicular lymphoma (FL) from 11 Surveillance, Epidemiology, and End Results (SEER) population-based registries during 1992 to 2006. Results Among patients without HIV/AIDS–related lymphoma, lung cancer risks were significantly elevated after CLL/SLL and FL but not after DLBCL (standardized incidence ratio [SIR], CLL/SLL = 1.42, FL = 1.28, DLBCL = 1.00; Poisson regression P for difference among subtypes, PDiff = .001). A similar pattern was observed for risk of cutaneous melanoma (SIR: CLL/SLL = 1.92, FL = 1.60, DLBCL = 1.06; PDiff = .004). Acute nonlymphocytic leukemia risks were significantly elevated after FL and DLBCL, particularly among patients receiving initial chemotherapy, but not after CLL/SLL (SIR: CLL/SLL = 1.13, FL = 5.96, DLBCL = 4.96; PDiff < .001). Patients with HIV/AIDS–related lymphoma (n = 932) were predominantly diagnosed with DLBCL and had significantly and substantially elevated risks for second anal cancer (SIR = 120.50) and Kaposi's sarcoma (SIR = 138.90). Conclusion Our findings suggest that differing immunologic alterations, treatments (eg, alkylating agent chemotherapy), genetic susceptibilities, and other risk factors (eg, viral infections, tobacco use) among lymphoma subtypes contribute to the patterns of second malignancy risk. Elucidating these patterns may provide etiologic clues to lymphoma as well as to the second malignancies. PMID:20940199

SMN transcript levels in leukocytes of SMA patients determined by absolute real-time PCR

PubMed Central

Tiziano, Francesco Danilo; Pinto, Anna Maria; Fiori, Stefania; Lomastro, Rosa; Messina, Sonia; Bruno, Claudio; Pini, Antonella; Pane, Marika; D'Amico, Adele; Ghezzo, Alessandro; Bertini, Enrico; Mercuri, Eugenio; Neri, Giovanni; Brahe, Christina

2010-01-01

Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder caused by homozygous mutations of the SMN1 gene. Three forms of SMA are recognized (type I–III) on the basis of clinical severity. All patients have at least one or more (usually 2–4) copies of a highly homologous gene (SMN2), which produces insufficient levels of functional SMN protein, because of alternative splicing of exon 7. Recently, evidence has been provided that SMN2 expression can be enhanced by pharmacological treatment. However, no reliable biomarkers are available to test the molecular efficacy of the treatments. At present, the only potential biomarker is the dosage of SMN products in peripheral blood. However, the demonstration that SMN full-length (SMN-fl) transcript levels are reduced in leukocytes of patients compared with controls remains elusive (except for type I). We have developed a novel assay based on absolute real-time PCR, which allows the quantification of SMN1-fl/SMN2-fl transcripts. For the first time, we have shown that SMN-fl levels are reduced in leukocytes of type II–III patients compared with controls. We also found that transcript levels are related to clinical severity as in type III patients SMN2-fl levels are significantly higher compared with type II and directly correlated with functional ability in type II patients and with age of onset in type III patients. Moreover, in haploidentical siblings with discordant phenotype, the less severely affected individuals showed significantly higher transcript levels. Our study shows that SMN2-fl dosage in leukocytes can be considered a reliable biomarker and can provide the rationale for SMN dosage in clinical trials. PMID:19603064

The effects of spectral tuning of evening ambient light on melatonin suppression, alertness and sleep.

PubMed

Rahman, Shadab A; St Hilaire, Melissa A; Lockley, Steven W

2017-08-01

We compared the effects of bedroom-intensity light from a standard fluorescent and a blue- (i.e., short-wavelength) depleted LED source on melatonin suppression, alertness, and sleep. Sixteen healthy participants (8 females) completed a 4-day inpatient study. Participants were exposed to blue-depleted circadian-sensitive (C-LED) light and a standard fluorescent light (FL, 4100K) of equal illuminance (50lx) for 8h prior to a fixed bedtime on two separate days in a within-subject, randomized, cross-over design. Each light exposure day was preceded by a dim light (<3lx) control at the same time 24h earlier. Compared to the FL condition, control-adjusted melatonin suppression was significantly reduced. Although subjective sleepiness was not different between the two light conditions, auditory reaction times were significantly slower under C-LED conditions compared to FL 30min prior to bedtime. EEG-based correlates of alertness corroborated the reduced alertness under C-LED conditions as shown by significantly increased EEG spectral power in the delta-theta (0.5-8.0Hz) bands under C-LED as compared to FL exposure. There was no significant difference in total sleep time (TST), sleep efficiency (SE%), and slow-wave activity (SWA) between the two conditions. Unlike melatonin suppression and alertness, a significant order effect was observed on all three sleep variables, however. Individuals who received C-LED first and then FL had increased TST, SE% and SWA averaged across both nights compared to individuals who received FL first and then C-LED. These data show that the spectral characteristics of light can be fine-tuned to attenuate non-visual responses to light in humans. Copyright © 2017 Elsevier Inc. All rights reserved.

Association between Global Biomarkers of Oxidative Stress and Hip Fracture in Postmenopausal Women: A Prospective Study

PubMed Central

Yang, Shuman; Feskanich, Diane; Willett, Walter C.; Eliassen, A. Heather; Wu, Tianying

2014-01-01

Human studies suggest that oxidative stress is a risk factor for osteoporosis, but its relationship with fracture risk is poorly understood. The purpose of the present study was to investigate the association between biomarkers of oxidative stress and hip fracture in postmenopausal women. We conducted a prospective study in the Nurses’ Health Study among 996 women aged 60 years or older at baseline blood collection in 1989–1990. Plasma fluorescent oxidation products (FlOPs) were measured at three excitation/emission wavelengths (360/420 nm named as FlOP_360; 320/420 nm named as FlOP_320 and 400/475 nm named as FlOP_400). FlOPs are generated from many different pathways (lipid, protein and DNA) and reflect a global oxidation burden. FlOP assay is 10–100 times more sensitive than measurement of malondialdehyde. We used Cox proportional hazards regression model to investigate the association between baseline plasma FlOPs and risk of hip fracture, adjusting for multiple hip fracture risk factors such as age, history of osteoporosis, history of hypertension, prior fracture and smoking status. Forty four hip fractures (4.4%) were identified during the follow-up (Maximum = 23 years). In the multivariable model, the hazard ratios (HR) of hip fracture in the second and third tertiles of FlOP_320 were 2.11 (95% confidence interval [CI] = 0.88–5.10) and 2.67 (95% CI = 1.14–6.27), respectively, in comparison with the lowest tertile, and the risk increased linearly with increasing FlOP_320 (P for trend = 0.021). Neither FlOP_360 nor FlOP_400 was significantly associated with risk of hip fracture (Tertile 3 versus tertile 1: HR = 0.70, 95% CI = 0.32–1.54, P for trend = 0.386 for FlOP_360; and HR = 0.88, 95% CI = 0.40–1.96, P for trend = 0.900 for FlOP_400). In this prospective study, higher plasma FlOP_320 was an independent risk factor for hip fracture. Our results need further confirmation. PMID:24957524

Correlation between bonding structure and microstructure in fullerenelike carbon nitride thin films

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gago, R.; Abendroth, B.; Moeller, W.

2005-03-15

The bonding structure of highly ordered fullerenelike (FL) carbon nitride (CN{sub x}) thin films has been assessed by x-ray absorption near-edge spectroscopy (XANES). Samples with different degrees of FL character have been analyzed to discern spectral signatures related to the FL microstructure. The XANES spectra of FL-CN{sub x} films resemble that of graphitic CN{sub x}, evidencing the sp{sup 2} hybridization of both C and N atoms. The FL structure is achieved with the promotion of N in threefold positions over pyridinelike and cyanidelike bonding environments. In addition, the relative {pi}{sup *}/{sigma}* XANES intensity ratio at the C(1s) edge is independentmore » of the FL character, while it decreases {approx}40% at the N(1s) edge with the formation of FL arrangements. This result indicates that there is no appreciable introduction of C-sp{sup 3} hybrids with the development of FL structures and, additionally, that a different spatial localization of {pi} electrons at C and N sites takes place in curved graphitic structures. The latter has implications for the elastic properties of graphene sheets and could, as such, explain the outstanding elastic properties of FL-CN{sub x}.« less

A CE-FL based method for real-time detection of in-capillary self-assembly of the nanoconjugates of polycysteine ligand and quantum dots.

PubMed

Wang, Jianhao; Zhu, Zhilan; Qiu, Lin; Wang, Jianpeng; Wang, Xiang; Xiao, Qicai; Xia, Jiang; Liu, Li; Liu, Xiaoqian; Feng, Wei; Wang, Jinmei; Miao, Peng; Gao, Liqian

2018-07-06

Small molecules with free thiol groups always show high binding affinity to quantum dots (QDs). However, it is still highly challenging to detect the binding capacity between thiol-containing molecules and QDs inside a capillary. To conquer this limitation, a capillary electrophoresis with fluorescence detection (CE-FL) based assay was proposed and established to investigate the binding capacity between QDs and a poly-thiolated peptide (ATTO 590-DDSSGGCCPGCC, ATTO-C4). Interestingly, the results showed that interval time had a great influence on QDs and ATTO-C4 self-assembly, which can be attributed to longer interval time benefitting the binding of QDs to ATTO-C4. The stability assays on ATTO-C4-QD assembly indicated that high concentration of imidazole or GSH had a high capability of competing with the bound ATTO-C4, evidenced by dramatically dropping of S 625 /S 565 ratio from 0.78 to 0.30 or 0.29. Therefore, all these results above suggested that this novel CE-FL based detection assay could be successfully applied to the binding studies between QDs and thiol-containing biomolecules.

A CE-FL based method for real-time detection of in-capillary self-assembly of the nanoconjugates of polycysteine ligand and quantum dots

NASA Astrophysics Data System (ADS)

Wang, Jianhao; Zhu, Zhilan; Qiu, Lin; Wang, Jianpeng; Wang, Xiang; Xiao, Qicai; Xia, Jiang; Liu, Li; Liu, Xiaoqian; Feng, Wei; Wang, Jinmei; Miao, Peng; Gao, Liqian

2018-07-01

Small molecules with free thiol groups always show high binding affinity to quantum dots (QDs). However, it is still highly challenging to detect the binding capacity between thiol-containing molecules and QDs inside a capillary. To conquer this limitation, a capillary electrophoresis with fluorescence detection (CE-FL) based assay was proposed and established to investigate the binding capacity between QDs and a poly-thiolated peptide (ATTO 590-DDSSGGCCPGCC, ATTO-C4). Interestingly, the results showed that interval time had a great influence on QDs and ATTO-C4 self-assembly, which can be attributed to longer interval time benefitting the binding of QDs to ATTO-C4. The stability assays on ATTO-C4-QD assembly indicated that high concentration of imidazole or GSH had a high capability of competing with the bound ATTO-C4, evidenced by dramatically dropping of S 625/S 565 ratio from 0.78 to 0.30 or 0.29. Therefore, all these results above suggested that this novel CE-FL based detection assay could be successfully applied to the binding studies between QDs and thiol-containing biomolecules.

Impact of vocal cord ultrasonography on endocrine surgery practices.

PubMed

Carneiro-Pla, Denise; Solorzano, Carmen C; Wilhelm, Scott M

2016-01-01

It is common practice to perform flexible laryngoscopy (FL) to ensure true vocal cord (TVC) mobility in patients with previous neck operations or patients with suspected VC dysfunction. Vocal cord ultrasonography (VCUS) is accurate in identifying TVC paralysis. The goal of this study is to evaluate the impact of VCUS as the initial study to confirm TVC mobility in patients requiring preoperative FL. A total of 194 consecutive patients with indications for preoperative FL underwent VCUS. In group 1, 52 patients had FL regardless of the results of VCUS, whereas in group 2, 142 patients had VCUS followed by FL only when VCUS was unsatisfactory. VCUS visualized TVC/arytenoids in 164 of 194 (85%) patients. TVC visualization was more common in women (95%) and in patients without thyroid cartilage calcification (92%) (P < .0005). VCUS predicted all paralyzed TVC. In group 2, 76% of patients had adequate VCUS and avoided preoperative FL. Among 24% of patients in whom VCUS was inadequate, 16 had preoperative FL attributable to a lack of TVC visualization, 6 had abnormal TVC mobility, 11 needed additional confirmations, and 2 had previous FL for another reason. VCUS changed surgeon practices by avoiding the need for preoperative FL in the majority of patients. This noninvasive and sensitive method demonstrates TVC mobility and safely precludes preoperative FL in most patients. Copyright © 2016 Elsevier Inc. All rights reserved.

Hepatitis C virus infection is an independent prognostic factor in follicular lymphoma

PubMed Central

Shimono, Joji; Miyoshi, Hiroaki; Kato, Takeharu; Sugio, Takeshi; Miyawaki, Kohta; Kamimura, Tomohiko; Miyagishima, Takuto; Eto, Tetsuya; Imaizumi, Yoshitaka; Kato, Koji; Nagafuji, Koji; Akashi, Koichi; Seto, Masao; Teshima, Takanori; Ohshima, Koichi

2018-01-01

Hepatitis C virus (HCV) is a single-stranded RNA virus that not only affects hepatocytes, by B cells as well. It is thought that HCV is involved in the onset of B-cell lymphoma. The clinicopathological characteristics of HCV-positive diffuse large B-cell lymphoma (DLBCL) and HCV-positive splenic marginal zone lymphoma (SMZL) are known, but there has been no report on HCV-positive follicular lymphoma (FL). In this study, the clinicopathological characteristics of HCV-positive FL were examined in 263 patients with FL who were classified into a HCV-positive group with HCV antibody and negative groups without one. The number of patients with HCV-positive FL and HCV-negative FL was 10 (3.8%) and 253 (96.2%), respectively. The patients with HCV-positive FL commonly had more than one region of lymphadenopathy, Ann Arbor stage III/IV, hemoglobin <120 g/l, elevated lactate dehydrogenase level, and high-risk categorization of Follicular Lymphoma International Prognostic Index (FLIPI) than in patients with HCV-negative FL. Overall survival and progression-free survival were poorer in patients with HCV-positive FL than in those with HCV-negative FL (p < 0.0001 and 0.006, respectively). Also, multivariate analysis revealed that positive HCV antibody was a poor prognostic factor of OS. In conclusion, HCV-positive FL has unique clinical features and may have a great impact on the overall survival of affected patients. PMID:29416725

Hepatitis C virus infection is an independent prognostic factor in follicular lymphoma.

PubMed

Shimono, Joji; Miyoshi, Hiroaki; Kato, Takeharu; Sugio, Takeshi; Miyawaki, Kohta; Kamimura, Tomohiko; Miyagishima, Takuto; Eto, Tetsuya; Imaizumi, Yoshitaka; Kato, Koji; Nagafuji, Koji; Akashi, Koichi; Seto, Masao; Teshima, Takanori; Ohshima, Koichi

2018-01-05

Hepatitis C virus (HCV) is a single-stranded RNA virus that not only affects hepatocytes, by B cells as well. It is thought that HCV is involved in the onset of B-cell lymphoma. The clinicopathological characteristics of HCV-positive diffuse large B-cell lymphoma (DLBCL) and HCV-positive splenic marginal zone lymphoma (SMZL) are known, but there has been no report on HCV-positive follicular lymphoma (FL). In this study, the clinicopathological characteristics of HCV-positive FL were examined in 263 patients with FL who were classified into a HCV-positive group with HCV antibody and negative groups without one. The number of patients with HCV-positive FL and HCV-negative FL was 10 (3.8%) and 253 (96.2%), respectively. The patients with HCV-positive FL commonly had more than one region of lymphadenopathy, Ann Arbor stage III/IV, hemoglobin <120 g/l, elevated lactate dehydrogenase level, and high-risk categorization of Follicular Lymphoma International Prognostic Index (FLIPI) than in patients with HCV-negative FL. Overall survival and progression-free survival were poorer in patients with HCV-positive FL than in those with HCV-negative FL ( p < 0.0001 and 0.006, respectively). Also, multivariate analysis revealed that positive HCV antibody was a poor prognostic factor of OS. In conclusion, HCV-positive FL has unique clinical features and may have a great impact on the overall survival of affected patients.

Suppression of Adaptive Immune Cell Activation Does Not Alter Innate Immune Adipose Inflammation or Insulin Resistance in Obesity.

PubMed

Subramanian, Manikandan; Ozcan, Lale; Ghorpade, Devram Sampat; Ferrante, Anthony W; Tabas, Ira

2015-01-01

Obesity-induced inflammation in visceral adipose tissue (VAT) is a major contributor to insulin resistance and type 2 diabetes. Whereas innate immune cells, notably macrophages, contribute to visceral adipose tissue (VAT) inflammation and insulin resistance, the role of adaptive immunity is less well defined. To address this critical gap, we used a model in which endogenous activation of T cells was suppressed in obese mice by blocking MyD88-mediated maturation of CD11c+ antigen-presenting cells. VAT CD11c+ cells from Cd11cCre+Myd88fl/fl vs. control Myd88fl/fl mice were defective in activating T cells in vitro, and VAT T and B cell activation was markedly reduced in Cd11cCre+Myd88fl/fl obese mice. However, neither macrophage-mediated VAT inflammation nor systemic inflammation were altered in Cd11cCre+Myd88fl/fl mice, thereby enabling a focused analysis on adaptive immunity. Unexpectedly, fasting blood glucose, plasma insulin, and the glucose response to glucose and insulin were completely unaltered in Cd11cCre+Myd88fl/fl vs. control obese mice. Thus, CD11c+ cells activate VAT T and B cells in obese mice, but suppression of this process does not have a discernible effect on macrophage-mediated VAT inflammation or systemic glucose homeostasis.

Use of Sodium Fluorescein in Meningioma Surgery Performed Under the YELLOW-560 nm Surgical Microscope Filter: Feasibility and Preliminary Results.

PubMed

Akçakaya, Mehmet Osman; Göker, Burcu; Kasımcan, Mustafa Ömür; Hamamcıoğlu, Mustafa Kemal; Kırış, Talat

2017-11-01

To evaluate the feasibility of sodium fluorescein (Na-Fl)-guided surgery involving the use of the PENTERO 900 surgical microscope equipped with the YELLOW-560 nm filter and low-dose Na-FL (200 mg/2-4 mg/kg) in meningioma surgery. The study included 30 patients with newly diagnosed or recurrent meningiomas who underwent Na-Fl-guided surgery between April 2015 and December 2016. Clinical features, surgical observations, extent of resection, and tumor histopathology were retrospectively analyzed. The Na-Fl enhancement pattern was assessed as "no enhancement," "diffuse homogenous enhancement," or "low heterogeneous enhancement." There were 30 meningiomas among the 30 patients. In 25 patients, Na-Fl was used for tumor demarcation, whereas in 5 patients, it was used for videoangiography. In this series, 88% of tumors showed diffuse homogeneous Na-Fl enhancement during the operation. The resection rate of the meningiomas was 87%. In 5 patients, in whom Na-Fl was used for videoangiography, the approach was useful to evaluate Na-Fl-stained vessels for patency and to understand their relationship with the tumor. No adverse events were encountered with regard to Na-Fl use. Na-Fl guidance with the use of the YELLOW-560 filter is safe and effective during meningioma surgery. Copyright © 2017 Elsevier Inc. All rights reserved.

Functioning of the Drosophila Wilms'-Tumor-1-Associated Protein Homolog, Fl(2)d, in Sex-Lethal-Dependent Alternative Splicing

PubMed Central

Penn, Jill K. M.; Graham, Patricia; Deshpande, Girish; Calhoun, Gretchen; Chaouki, Ahmad Sami; Salz, Helen K.; Schedl, Paul

2008-01-01

fl(2)d, the Drosophila homolog of Wilms'-tumor-1-associated protein (WTAP), regulates the alternative splicing of Sex-lethal (Sxl), transformer (tra), and Ultrabithorax (Ubx). Although WTAP has been found in functional human spliceosomes, exactly how it contributes to the splicing process remains unknown. Here we attempt to identify factors that interact genetically and physically with fl(2)d. We begin by analyzing the Sxl-Fl(2)d protein–protein interaction in detail and present evidence suggesting that the female-specific fl(2)d1 allele is antimorphic with respect to the process of sex determination. Next we show that fl(2)d interacts genetically with early acting general splicing regulators and that Fl(2)d is present in immunoprecipitable complexes with Snf, U2AF50, U2AF38, and U1-70K. By contrast, we could not detect Fl(2)d complexes containing the U5 snRNP protein U5-40K or with a protein that associates with the activated B spliceosomal complex SKIP. Significantly, the genetic and molecular interactions observed for Sxl are quite similar to those detected for fl(2)d. Taken together, our findings suggest that Sxl and fl(2)d function to alter splice-site selection at an early step in spliceosome assembly. PMID:18245840

Accelerated Fatigue Resistance of Thick CAD/CAM Composite Resin Overlays Bonded with Light- and Dual-polymerizing Luting Resins.

PubMed

Goldberg, Jack; Güth, Jan-Frederik; Magne, Pascal

To evaluate the accelerated fatigue resistance of thick CAD/CAM composite resin overlays luted with three different bonding methods. Forty-five sound human second mandibular molars were organized and distributed into three experimental groups. All teeth were restored with a 5-mm-thick CAD/CAM composite resin overlay. Group A: immediate dentin sealing (IDS) with Optibond FL and luted with light-polymerizing composite (Herculite XRV). Group B: IDS with Optibond FL and luted with dual-polymerizing composite (Nexus 3). Group C: direct luting with Optibond FL and dual-polymerizing composite (Nexus 3). Masticatory forces at a frequency of 5 Hz were simulated using closed-loop servo-hydraulics and forces starting with a load of 200 N for 5000 cycles, followed by steps of 400, 600, 800, 1000, 1200 and 1400 N for a maximum of 30,000 cycles. Each step was applied through a flat steel cylinder at a 45-degree angle under submerged conditions. The fatigue test generated one failure in group A, three failures in group B, and no failures in group C. The survival table analysis for the fatigue test did not demonstrate any significant difference between the groups (p = 0.154). The specimens that survived the fatigue test were set up for the load-to-failure test with a limit of 4600 N. The survival table analysis for the load-to-failure test demonstrates an average failure load of 3495.20 N with survival of four specimens in group A, an average failure load of 4103.60 N with survival of six specimens in group B, and an average failure load of 4075.33 N with survival of nine specimens in group C. Pairwise comparisons revealed no significant differences (p < 0.016 after Bonferroni correction). Within the limitations of this in vitro study, it can be concluded that although the dual-polymerizing luting material seems to provide better results under extreme conditions, light-polymerizing luting composites in combination with IDS are not contraindicated with thick restorations.

Development of a novel fluorogenic proteolytic beacon for in vivo detection and imaging of tumour-associated matrix metalloproteinase-7 activity.

PubMed Central

McIntyre, J Oliver; Fingleton, Barbara; Wells, K Sam; Piston, David W; Lynch, Conor C; Gautam, Shiva; Matrisian, Lynn M

2004-01-01

The present study describes the in vivo detection and imaging of tumour-associated MMP-7 (matrix metalloproteinase-7 or matrilysin) activity using a novel polymer-based fluorogenic substrate PB-M7VIS, which serves as a selective 'proteolytic beacon' (PB) for this metalloproteinase. PB-M7VIS is built on a PAMAM (polyamido amino) dendrimer core of 14.2 kDa, covalently coupled with an Fl (fluorescein)-labelled peptide Fl(AHX)RPLALWRS(AHX)C (where AHX stands for aminohexanoic acid) and with TMR (tetramethylrhodamine). PB-M7VIS is efficiently and selectively cleaved by MMP-7 with a k (cat)/ K (m) value of 1.9x10(5) M(-1).s(-1) as measured by the rate of increase in Fl fluorescence (up to 17-fold for the cleavage of an optimized PB-M7VIS) with minimal change in the TMR fluorescence. The K (m) value for PB-M7VIS is approx. 0.5 microM, which is approx. two orders of magnitude lower when compared with that for an analogous soluble peptide, indicating efficient interaction of MMP-7 with the synthetic polymeric substrate. With MMP-2 or -3, the k (cat)/ K (m) value for PB-M7VIS is approx. 56- or 13-fold lower respectively, when compared with MMP-7. In PB-M7VIS, Fl(AHX)RPLALWRS(AHX)C is a selective optical sensor of MMP-7 activity and TMR serves to detect both the uncleaved and cleaved reagents. Each of these can be visualized as subcutaneous fluorescent phantoms in a mouse and optically discriminated based on the ratio of green/red (Fl/TMR) fluorescence. The in vivo specificity of PB-M7VIS was tested in a mouse xenograft model. Intravenous administration of PB-M7VIS gave significantly enhanced Fl fluorescence from MMP-7-positive tumours, but not from control tumours ( P <0.0001), both originally derived from SW480 human colon cancer cells. Prior systemic treatment of the tumour-bearing mice with an MMP inhibitor BB-94 ([4-( N -hydroxyamino)-2 R -isobutyl-3 S -(thienylthiomethyl)-succinyl]-L-phenylalanine- N -methylamide), markedly decreased the Fl fluorescence over the MMP-7-positive tumour by approx. 60%. Thus PB-M7VIS functions as a PB for in vivo detection of MMP-7 activity that serves to light this optical beacon and is, therefore, a selective in vivo optical molecular imaging contrast reagent. PMID:14556651

Development and Application of the Remote Food Photography Method to Measure Food Intake in Exclusively Milk Fed Infants: A Laboratory-Based Study.

PubMed

Altazan, Abby D; Gilmore, L Anne; Burton, Jeffrey H; Ragusa, Shelly A; Apolzan, John W; Martin, Corby K; Redman, Leanne M

Accurate methods of assessing food intake in infants are needed to assess the relationship between infant feeding practices and risk of childhood obesity. Current methods are either subjective or have limited ability for use beyond clinical research settings. To assess the accuracy of the RFPM to evaluate simulated milk intake including energy, macronutrient, and micronutrient intake compared to direct weighing within a controlled study. Individuals were recruited to prepare three 2 fl oz, 4 fl oz, 6 fl oz, and 8 fl oz servings of infant formula and to capture photographs at different stages of preparation (dry powdered formula, prepared formula, and liquid waste) using the SmartIntake® application. Gram weights of the bottles were obtained by the RFPM and direct weighing. Using the United States Department of Agriculture National Nutrient Database for Standard Reference, energy, macronutrient, and micronutrient values were generated from gram weights. Intake of formula prepared from powder measured by the RFPM was equivalent to weighed intake within 7.5% equivalence bounds among all servings and each serving size. The mean difference between methods varied among servings sizes with the RFPM underestimating intake by 1.6 ± 0.4 kcals in 2 fl oz servings, 4.8 ± 0.6 kcals in 4 fl oz servings, and 6.2 ± 1.0 kcals in 6 fl oz servings, and overestimating intake by 0.1 ± 1.2 kcals in 8 fl oz servings. Bland-Altman analysis showed that the RFPM overestimated intake at lower levels food intake and underestimated intake at higher levels. Considering photographs of only the prepared formula bottle and the bottle with formula waste to simulate ready-to-feed formula and human breast milk, intake estimated by the RFPM was equivalent to the directly weighed intake within 7.5% for all servings. The RFPM has higher accuracy than subjective methods and similar accuracy as compared to the objective methods in estimating simulated intake of milk and formula with lower burden to caregivers. The RFPM is a viable method for measuring intake in exclusively milk fed infants by caregivers in a controlled environment with potential for widespread use in research and clinical practice.

Development and Application of the Remote Food Photography Method to Measure Food Intake in Exclusively Milk Fed Infants: A Laboratory-Based Study

PubMed Central

Altazan, Abby D.; Gilmore, L. Anne; Burton, Jeffrey H.; Ragusa, Shelly A.; Apolzan, John W.; Martin, Corby K.; Redman, Leanne M.

2016-01-01

Background Accurate methods of assessing food intake in infants are needed to assess the relationship between infant feeding practices and risk of childhood obesity. Current methods are either subjective or have limited ability for use beyond clinical research settings. Objective To assess the accuracy of the RFPM to evaluate simulated milk intake including energy, macronutrient, and micronutrient intake compared to direct weighing within a controlled study. Methods Individuals were recruited to prepare three 2 fl oz, 4 fl oz, 6 fl oz, and 8 fl oz servings of infant formula and to capture photographs at different stages of preparation (dry powdered formula, prepared formula, and liquid waste) using the SmartIntake® application. Gram weights of the bottles were obtained by the RFPM and direct weighing. Using the United States Department of Agriculture National Nutrient Database for Standard Reference, energy, macronutrient, and micronutrient values were generated from gram weights. Results Intake of formula prepared from powder measured by the RFPM was equivalent to weighed intake within 7.5% equivalence bounds among all servings and each serving size. The mean difference between methods varied among servings sizes with the RFPM underestimating intake by 1.6 ± 0.4 kcals in 2 fl oz servings, 4.8 ± 0.6 kcals in 4 fl oz servings, and 6.2 ± 1.0 kcals in 6 fl oz servings, and overestimating intake by 0.1 ± 1.2 kcals in 8 fl oz servings. Bland-Altman analysis showed that the RFPM overestimated intake at lower levels food intake and underestimated intake at higher levels. Considering photographs of only the prepared formula bottle and the bottle with formula waste to simulate ready-to-feed formula and human breast milk, intake estimated by the RFPM was equivalent to the directly weighed intake within 7.5% for all servings. Conclusions The RFPM has higher accuracy than subjective methods and similar accuracy as compared to the objective methods in estimating simulated intake of milk and formula with lower burden to caregivers. The RFPM is a viable method for measuring intake in exclusively milk fed infants by caregivers in a controlled environment with potential for widespread use in research and clinical practice. PMID:27684949

Weather Avoidance Guidelines for NASA Global Hawk High-Altitude UAS

NASA Technical Reports Server (NTRS)

Cecil, Daniel J.; Zipser, Edward J.; Velden, Chris; Monette, Sarah; Heymsfield, Gerry; Braun, Scott; Newman, Paul; Black, Pete; Black, Michael; Dunion, Jason

2014-01-01

NASA operates two Global Hawk unmanned aircraft systems for Earth Science research projects. In particular, they are used in the Hurricane and Severe Storm Sentinel (HS3) project during 2012, 2013, and 2014 to take measurements from the environment around tropical cyclones, and from directly above tropical cyclones. There is concern that strict adherence to the weather avoidance rules used in 2012 may sacrifice the ability to observe important science targets. We have proposed modifications to these weather avoidance rules that we believe will improve the ability to observe science targets without compromising aircraft safety. The previous guidelines, used in 2012, specified: Do not approach thunderstorms within 25 nm during flight at FL500 or below. When flying above FL500: Do not approach reported lightning within 25NM in areas where cloud tops are reported at FL500 or higher. Aircraft should maintain at least 10000 ft vertical separation from reported lightning if cloud tops are below FL500. No over-flight of cumulus tops higher than FL500. No flight into forecast or reported icing conditions. No flight into forecast or reported moderate or severe turbulence Based on past experience with high-altitude flights over tropical cyclones, we have recommended changing this guidance to: Do not approach thunderstorms within 25 nm during flight at FL500 or below. Aircraft should maintain at least 5000 ft vertical separation from significant convective cloud tops except: a) When cloud tops above FL500: In the event of reported significant lightning activity or indicators of significant overshooting tops, do not approach within 10-25 nm, depending on pilot discretion and advice from Mission Scientist. b) When cloud tops are below FL500, maintain 10000 ft separation from reported significant lightning or indicators of significant overshooting tops. No flight into forecasted or reported icing conditions. No flight into forecasted or reported moderate or severe turbulence The key changes have to do with overflight of high convective cloud tops and those producing lightning. Experience shows that most tropical oceanic convection (including that in tropical cyclones) is relatively gentle even if the cloud tops are quite high, and can be safely overflown. Exceptions are convective elements producing elevated lightning flash rates (more than just the occasional flash, which would trigger avoidance under the previous rules) and significant overshooting cloud tops.

Multi-parameter analysis using photovoltaic cell-based optofluidic cytometer

PubMed Central

Yan, Chien-Shun; Wang, Yao-Nan

2016-01-01

A multi-parameter optofluidic cytometer based on two low-cost commercial photovoltaic cells and an avalanche photodetector is proposed. The optofluidic cytometer is fabricated on a polydimethylsiloxane (PDMS) substrate and is capable of detecting side scattered (SSC), extinction (EXT) and fluorescence (FL) signals simultaneously using a free-space light transmission technique without the need for on-chip optical waveguides. The feasibility of the proposed device is demonstrated by detecting fluorescent-labeled polystyrene beads with sizes of 3 μm, 5 μm and 10 μm, respectively, and label-free beads with a size of 7.26 μm. The detection experiments are performed using both single-bead population samples and mixed-bead population samples. The detection results obtained using the SSC/EXT, EXT/FL and SSC/FL signals are compared with those obtained using a commercial flow cytometer. It is shown that the optofluidic cytometer achieves a high detection accuracy for both single-bead population samples and mixed-bead population samples. Consequently, the proposed device provides a versatile, straightforward and low-cost solution for a wide variety of point-of-care (PoC) cytometry applications. PMID:27699122

75 FR 82228 - IFR Altitudes; Miscellaneous Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-30

... Is Amended To Read in Part Shirt, OH FIX *Gloom, OH FIX 3000 *4000--MRA *Gloom, OH FIX Appleton, OH VORTAC..... 3000 *4000--MRA Appleton, OH VORTAC Mansfield, OH VORTAC.... 3000 Mansfield, OH VORTAC Dryer... in Part Lee County, FL VORTAC Jocks, FL FIX 2600 Jocks, FL FIX *Crowd, FL FIX **2300 *5000--MRA...

Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169

EPA Science Inventory

We report the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169T, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, though Fl-016...

77 FR 38384 - Qualification of Drivers; Exemption Applications; Vision

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-27

... S. Johannsen (IA) Charles E. Johnston (MO) Harry L. Jones (OH) Mearl C. Kennedy (OH) Aaron C...) Bobby G. Minton (NC) Charles J. Morman (FL) Larry A. Nienhuis (MI) Corey L. Paraf (IL) Ronald M. Price (MD) John P. Raftis (FL) Scott D. Russell (WI) Alton M. Rutherford (FL) Charles L. Schnell (FL) Andrew...

33 CFR 80.760 - Horeshoe Point, FL to Rock Island, FL.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Horeshoe Point, FL to Rock Island... Rock Island, FL. (a) Except inside lines specifically described provided in this section, the 72 COLREGS shall apply on the bays, bayous, creeks, marinas, and rivers from Horseshoe Point to the Rock...

33 CFR 80.760 - Horeshoe Point, FL to Rock Island, FL.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Horeshoe Point, FL to Rock Island... Rock Island, FL. (a) Except inside lines specifically described provided in this section, the 72 COLREGS shall apply on the bays, bayous, creeks, marinas, and rivers from Horseshoe Point to the Rock...

33 CFR 80.760 - Horeshoe Point, FL to Rock Island, FL.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Horeshoe Point, FL to Rock Island... Rock Island, FL. (a) Except inside lines specifically described provided in this section, the 72 COLREGS shall apply on the bays, bayous, creeks, marinas, and rivers from Horseshoe Point to the Rock...

33 CFR 80.760 - Horeshoe Point, FL to Rock Island, FL.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Horeshoe Point, FL to Rock Island... Rock Island, FL. (a) Except inside lines specifically described provided in this section, the 72 COLREGS shall apply on the bays, bayous, creeks, marinas, and rivers from Horseshoe Point to the Rock...

33 CFR 80.760 - Horeshoe Point, FL to Rock Island, FL.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Horeshoe Point, FL to Rock Island... Rock Island, FL. (a) Except inside lines specifically described provided in this section, the 72 COLREGS shall apply on the bays, bayous, creeks, marinas, and rivers from Horseshoe Point to the Rock...

77 FR 21662 - Amendment of Class D Airspace; Cocoa Beach, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-11

...-0099; Airspace Docket No. 12-ASO-11] Amendment of Class D Airspace; Cocoa Beach, FL AGENCY: Federal... Class D airspace at Cape Canaveral Skid Strip, Cocoa Beach, FL, by correcting the geographic coordinates... of Cape Canaveral Skid Strip, Cocoa Beach, FL. Also, the geographic coordinates for the airport need...

78 FR 38554 - Amendment of Class E Airspace; Live Oak, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-27

... Live Oak, FL area, as new Standard Instrument Approach Procedures (SIAPs) have been developed at... from 700 feet above the surface within a 7-mile radius at Suwannee County Airport, Live Oak, FL. New...-0001; Airspace Docket No. 12-ASO-45] Amendment of Class E Airspace; Live Oak, FL AGENCY: Federal...

33 CFR 80.750 - Sanibel Island, FL to St. Petersburg, FL.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Sanibel Island, FL to St... SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Gulf Coast § 80.750 Sanibel Island, FL to..., between Captiva Island and Sanibel Island, and lines drawn across Redfish and Captiva Passes parallel to...

Protection of Flos Lonicerae against acetaminophen-induced liver injury and its mechanism.

PubMed

Jiang, Ping; Sheng, Yu-chen; Chen, Yu-hao; Ji, Li-li; Wang, Zheng-tao

2014-11-01

This study aims to observe the protective action of Flos Lonicerae (FL) aqueous extract against acetaminophen (AP)-induced liver injury and its mechanism. Results show that FL decreases AP-increased serum alanine/aspartate transaminases (ALT/AST) activity, as well as total bilirubin (TB) amount, in mice. Histological evaluation of the liver further confirms the protection of FL against AP-induced hepatotoxicity. TdT-mediated biotin-dUTP nick-end labeling (TUNEL) assay shows that FL reduces AP-increased apoptotic cells. Furthermore, AP-decreased liver glutamate-cysteine ligase (GCL) enzymatic activity and glutathione (GSH) amount are both reversed by FL because of the increased expression of the catalytic subunit of GCL (GCLC) protein. The amount of chlorogenic acid (CGA), caffeic acid, and luteolin, the main active compounds in FL, is detected by high-performance liquid chromatography (HPLC). In addition, cell viability assay demonstrates that polyphenols in FL, such as CGA, caffeic acid, as well as isochlorogenic acids A, B, and C, can reverse AP-induced cytotoxicity. In conclusion, FL can prevent AP-induced liver injury by inhibiting apoptosis. The cellular antioxidant enzyme GCL is also involved in such protection. Polyphenols may be the main active hepato-protective ingredients in FL. Copyright © 2014 Elsevier B.V. All rights reserved.

Dietary 2’-Fucosyllactose Enhances Operant Conditioning and Long-Term Potentiation via Gut-Brain Communication through the Vagus Nerve in Rodents

PubMed Central

Vazquez, Enrique; Barranco, Alejandro; Ramirez, Maria; Gruart, Agnes; Delgado-Garcia, Jose M.; Jimenez, Maria L.; Buck, Rachael; Rueda, Ricardo

2016-01-01

2´-fucosyllactose (2´-FL) is an abundant human milk oligosaccharide (HMO) in human milk with diverse biological effects. We recently reported ingested 2´-FL stimulates central nervous system (CNS) function, such as hippocampal long term potentiation (LTP) and learning and memory in rats. Conceivably the effect of 2´-FL on CNS function may be via the gut-brain axis (GBA), specifically the vagus nerve, and L-fucose (Fuc) may play a role. This study had two aims: (1) determine if the effect of ingested 2´-FL on the modulation of CNS function is dependent on the integrity of the molecule; and (2) confirm if oral 2´-FL modified hippocampal LTP and associative learning related skills in rats submitted to bilateral subdiaphragmatic vagotomy. Results showed that 2´-FL but not Fuc enhanced LTP, and vagotomy inhibited the effects of oral 2´-FL on LTP and associative learning related paradigms. Taken together, the data show that dietary 2´-FL but not its Fuc moiety affects cognitive domains and improves learning and memory in rats. This effect is dependent on vagus nerve integrity, suggesting GBA plays a role in 2´-FL-mediated cognitive benefits. PMID:27851789

DC-SIGN–expressing macrophages trigger activation of mannosylated IgM B-cell receptor in follicular lymphoma

PubMed Central

Amin, Rada; Mourcin, Frédéric; Uhel, Fabrice; Pangault, Céline; Ruminy, Philippe; Dupré, Loic; Guirriec, Marion; Marchand, Tony; Fest, Thierry; Lamy, Thierry

2015-01-01

Follicular lymphoma (FL) results from the accumulation of malignant germinal center (GC) B cells leading to the development of an indolent and largely incurable disease. FL cells remain highly dependent on B-cell receptor (BCR) signaling and on a specific cell microenvironment, including T cells, macrophages, and stromal cells. Importantly, FL BCR is characterized by a selective pressure to retain surface immunoglobulin M (IgM) BCR despite an active class-switch recombination process, and by the introduction, in BCR variable regions, of N-glycosylation acceptor sites harboring unusual high-mannose oligosaccharides. However, the relevance of these 2 FL BCR features for lymphomagenesis remains unclear. In this study, we demonstrated that IgM+ FL B cells activated a stronger BCR signaling network than IgG+ FL B cells and normal GC B cells. BCR expression level and phosphatase activity could both contribute to such heterogeneity. Moreover, we underlined that a subset of IgM+ FL samples, displaying highly mannosylated BCR, efficiently bound dendritic cell–specific intercellular adhesion molecule-3–grabbing nonintegrin (DC-SIGN), which could in turn trigger delayed but long-lasting BCR aggregation and activation. Interestingly, DC-SIGN was found within the FL cell niche in situ. Finally, M2 macrophages induced a DC-SIGN–dependent adhesion of highly mannosylated IgM+ FL B cells and triggered BCR-associated kinase activation. Interestingly, pharmacologic BCR inhibitors abolished such crosstalk between macrophages and FL B cells. Altogether, our data support an important role for DC-SIGN–expressing infiltrating cells in the biology of FL and suggest that they could represent interesting therapeutic targets. PMID:26272216

Activation of liver X receptor decreases atherosclerosis in Ldlr⁻/⁻ mice in the absence of ATP-binding cassette transporters A1 and G1 in myeloid cells.

PubMed

Kappus, Mojdeh S; Murphy, Andrew J; Abramowicz, Sandra; Ntonga, Vusisizwe; Welch, Carrie L; Tall, Alan R; Westerterp, Marit

2014-02-01

Liver X receptor (LXR) activators decrease atherosclerosis in mice. LXR activators (1) directly upregulate genes involved in reverse cholesterol transport and (2) exert anti-inflammatory effects mediated by transrepression of nuclear factor-κB target genes. We investigated whether myeloid cell deficiency of ATP-binding cassette transporters A1 and G1 (ABCA1/G1), principal targets of LXR that promote macrophage cholesterol efflux and initiate reverse cholesterol transport, would abolish the beneficial effects of LXR activation on atherosclerosis. LXR activator T0901317 substantially reduced inflammatory gene expression in macrophages lacking ABCA1/G1. Ldlr(-/-) mice were transplanted with Abca1(-/-)Abcg1(-/-) or wild-type bone marrow (BM) and fed a Western-type diet for 6 weeks with or without T0901317 supplementation. Abca1/g1 BM deficiency increased atherosclerotic lesion complexity and inflammatory cell infiltration into the adventitia and myocardium. T0901317 markedly decreased lesion area, complexity, and inflammatory cell infiltration in the Abca1(-/-)Abcg1(-/-) BM-transplanted mice. To investigate whether this was because of macrophage Abca1/g1 deficiency, Ldlr(-/-) mice were transplanted with LysmCreAbca1(fl/fl)Abcg1(fl/fl) or Abca1(fl/fl)Abcg1(fl/fl) BM and fed Western-type diet with or without the more specific LXR agonist GW3965 for 12 weeks. GW3965 decreased lesion size in both groups, and the decrease was more prominent in the LysmCreAbca1(fl/fl)Abcg1(fl/fl) group. The results suggest that anti-inflammatory effects of LXR activators are of key importance to their antiatherosclerotic effects in vivo independent of cholesterol efflux pathways mediated by macrophage ABCA1/G1. This has implications for the development of LXR activators that lack adverse effects on lipogenic genes while maintaining the ability to transrepress inflammatory genes.

SHIP-1 Deficiency in AID+ B Cells Leads to the Impaired Function of B10 Cells with Spontaneous Autoimmunity.

PubMed

Chen, Yingjia; Hu, Fanlei; Dong, Xuejiao; Zhao, Meng; Wang, Jing; Sun, Xiaolin; Kim, Tae Jin; Li, Zhanguo; Liu, Wanli

2017-11-01

Unlike conventional B cells, regulatory B cells exhibit immunosuppressive functions to downregulate inflammation via IL-10 production. However, the molecular mechanism regulating the production of IL-10 is not fully understood. In this study, we report the finding that activation-induced cytidine deaminase (AID) is highly upregulated in the IL-10-competent B cell (B10) cell from Innp5d fl/fl Aicda Cre/+ mice, whereas the 5' inositol phosphatase SHIP-1 is downregulated. Notably, SHIP-1 deficiency in AID + B cells leads to a reduction in cell count and impaired IL-10 production by B10 cells. Furthermore, the Innp5d fl/fl Aicda Cre/+ mouse model shows B cell-dependent autoimmune lupus-like phenotypes, such as elevated IgG serum Abs, formation of spontaneous germinal centers, production of anti-dsDNA and anti-nuclear Abs, and the obvious deposition of IgG immune complexes in the kidney with age. We observe that these lupus-like phenotypes can be reversed by the adoptive transfer of B10 cells from control Innp5d fl/fl mice, but not from the Innp5d fl/fl Aicda Cre/+ mice. This finding highlights the importance of defective B10 cells in Innp5d fl/fl Aicda Cre/+ mice. Whereas p-Akt is significantly upregulated, MAPK and AP-1 activation is impaired in B10 cells from Innp5d fl/fl Aicda Cre/+ mice, resulting in the reduced production of IL-10. These results show that SHIP-1 is required for the maintenance of B10 cells and production of IL-10, and collectively suggests that SHIP-1 could be a new potential therapeutic target for the treatment of autoimmune diseases. Copyright © 2017 by The American Association of Immunologists, Inc.

Reduced Cx43 expression triggers increased fibrosis due to enhanced fibroblast activity.

PubMed

Jansen, John A; van Veen, Toon A B; de Jong, Sanne; van der Nagel, Roel; van Stuijvenberg, Leonie; Driessen, Helen; Labzowski, Ronald; Oefner, Carolin M; Bosch, Astrid A; Nguyen, Tri Q; Goldschmeding, Roel; Vos, Marc A; de Bakker, Jacques M T; van Rijen, Harold V M

2012-04-01

Arrhythmogenic ventricular remodeling is hallmarked by both reduced gap junction expression and increased collagen deposition. We hypothesized that reduced connexin43 (Cx43) expression is responsible for enhanced fibrosis in the remodeled heart, resulting in an arrhythmogenic substrate. Therefore, we investigated the effect of normal or reduced Cx43 expression on the formation of fibrosis in a physiological (aging) and pathophysiological (transverse aortic constriction [TAC]) mouse model. The Cx43(fl/fl) and Cx43(CreER(T)/fl) mice were aged 18 to 21 months or, at the age of 3 months, either TAC or sham operated and euthanized after 16 weeks. Epicardial activation mapping of the right and left ventricles was performed on Langendorff perfused hearts. Sustained ventricular arrhythmias were induced in 0 of 11 aged Cx43(fl/fl) and 10 of 15 Cx43(Cre-ER(T)/fl) mice (P<0.01). Cx43 expression was reduced by half in aged Cx43(CreER(T)/fl) compared with aged Cx43(fl/fl) mice, whereas collagen deposition was significantly increased from 1.1±0.2% to 7.4±1.3%. Aged Cx43(CreER(T)/fl) mice with arrhythmias had significantly higher levels of fibrosis and conduction heterogeneity than aged Cx43(CreER(T)/fl) mice without arrhythmias. The TAC operation significantly increased fibrosis in control compared with sham (4.0±1.2% versus 0.4±0.06%), but this increase was significantly higher in Cx43(CreER(T)/fl) mice (10.8±1.4%). Discoidin domain receptor 2 expression was unchanged, but procollagen peptide I and III expression and collagen type 1α2 mRNA levels were higher in TAC-operated Cx43HZ mice. Reduced cellular coupling results in more excessive collagen deposition during aging or pressure overload in mice due to enhanced fibroblast activity, leading to increased conduction in homogeneity and proarrhythmia.

The t(14;18) translocation is absent from endothelial and follicular dendritic cells of follicular lymphoma (FL) and shows heterogeneous presence in preserved FL mantle zones.

PubMed

Kosmidis, Perikles; Mankel, Barbara; Fend, Falko; Adam, Patrick

2018-05-02

The translocation t(14;18)(q32;q21) is the genetic hallmark of follicular lymphoma (FL) and can be observed in 85-90% of cases. Whether the translocation is restricted to cells with germinal center B-cell phenotype or can be observed in other cell types of the microenvironment remains debated. Of interest, cases of associated histiocytic and dendritic cell sarcomas arising in the background of FL have been shown to be clonally related and carry the t(14;18), suggesting a "transdifferentiation" of the malignant FL clone into a neoplasm of a different hematopoietic lineage. We analyzed the presence of the t(14;18)(q32;q21) as a surrogate marker of the malignant clone in cells of the FL microenvironment using combined fluorescence immunophenotyping and interphase cytogenetics targeting the BCL2 gene locus. In addition to non-lymphoid cells in FL, we analysed FL with preserved IgD+ mantle zones and cases of in situ follicular neoplasia (ISFN) to investigate whether cells of non-germinal center B-cell phenotype are part of the malignant clone. Six (40%) of 15 manifest FL cases with preserved IgD+ mantle zones did not harbour the t(14;18)(q32;q21) translocation. In all t(14;18) + FL cases, follicular dendritic cells and endothelial cells lacked the t(14;18) translocation. 2/9 FL revealed t(14;18)- IgD+ mantle zone B-cells. In the seven ISFN cases, the t(14;18) translocation was strictly confined to germinal center cells. The t(14;18) translocation in follicular lymphoma is limited to B-cells. The origin of IgD+ mantle cells is heterogeneous, in the majority of cases belonging to the neoplastic clone, whereas a minority of cases of manifest FL show nonneoplastic mantle zones, similar to ISFN.

Effect of location of glass fiber-reinforced composite reinforcement on the flexural properties of a maxillary complete denture in vitro.

PubMed

Takahashi, Yutaka; Yoshida, Kaneyoshi; Shimizu, Hiroshi

2011-07-01

Objective. To evaluate the effect of the location of glass fiber-reinforced composite (FRC) reinforcement on the flexural load at the proportional limit (FL-PL) and the flexural deflection of a maxillary acrylic resin complete denture. Material and methods. Maxillary acrylic resin complete dentures strengthened with and without FRC reinforcement were tested. The polymerized FRC was embedded in the denture base resin in the doughy state and placed (1) under the ridge lap region, (2) in the anterior region, (3) in the middle region or (4) in the anterior and posterior regions. The FL-PL and flexural deflection value at the 100-N loading point of the reinforced maxillary denture specimens were tested. Results. All of the reinforced dentures had a higher FL-PL than the denture without reinforcement but the FL-PL values of all the dentures were not significantly different from each other. The efficiency of the FRC reinforcement compared to the unreinforced denture was 1.54-1.75 times greater. All of the reinforced dentures showed significantly lower deflection compared to the unreinforced denture, but the flexural deflections of all the dentures were not significantly different from each other. Conclusions. The location of the FRC reinforcement did not affect the fracture resistance of the maxillary acrylic resin complete denture. All of the reinforced dentures had higher FL-PL and lower flexural deflection than the denture without reinforcement.

A leukemic double-hit follicular lymphoma associated with a complex variant translocation, t(8;14;18)(q24;q32;q21), involving BCL2, MYC, and IGH.

PubMed

Minakata, Daisuke; Sato, Kazuya; Ikeda, Takashi; Toda, Yumiko; Ito, Shoko; Mashima, Kiyomi; Umino, Kento; Nakano, Hirofumi; Yamasaki, Ryoko; Morita, Kaoru; Kawasaki, Yasufumi; Sugimoto, Miyuki; Yamamoto, Chihiro; Ashizawa, Masahiro; Hatano, Kaoru; Oh, Iekuni; Fujiwara, Shin-Ichiro; Ohmine, Ken; Kawata, Hirotoshi; Muroi, Kazuo; Miura, Ikuo; Kanda, Yoshinobu

2018-01-01

Double-hit lymphoma (DHL) is defined as lymphoma with concurrent BCL2 and MYC translocations. While the most common histological subtype of DHL is diffuse large B-cell lymphoma, the present patient had leukemic follicular lymphoma (FL). A 52-year-old man was admitted to our hospital due to general fatigue and cervical and inguinal lymph node swelling. The patient was leukemic and the pathological diagnosis of the inguinal lymph node was FL grade 1. Chromosomal analysis revealed a complex karyotype including a rare three-way translocation t(8;14;18)(q24;q32;q21) involving the BCL2, MYC, and IGH genes. Based on a combination of fluorescence in situ hybridization (FISH), using BCL2, MYC and IGH, and spectral karyotyping (SKY), the karyotype was interpreted as being the result of a multistep mechanism in which the precursor B-cell gained t(14;18) in the bone marrow and acquired a translocation between der(14)t(14;18) and chromosome 8 in the germinal center, resulting in t(8;14;18). The pathological diagnosis was consistently FL, not only at presentation but even after a second relapse. The patient responded well to standard chemotherapies but relapsed after a short remission. This patient is a unique case of leukemic DH-FL with t(8;14;18) that remained in FL even at a second relapse. Copyright © 2017 Elsevier Inc. All rights reserved.

An open-label trial examining the efficacy and safety of a pre- and postprocedure topical five-product system (Clinique Medical Optimizing Regimen) specifically formulated to complement laser/light-based facial cosmetic procedures.

PubMed

Narurkar, Vic A; Beer, Kenneth R; Cohen, Joel L

2010-12-01

Specialized skin care regimens may help to minimize adverse events (AEs) following non-ablative facial procedures. A 14-week, open-label, three-center study evaluated the efficacy and safety of a topical five-product system (Clinique Medical Optimizing Regimen; Allergan, Inc., Irvine, CA, USA) for minimizing localized AEs during two 6-week procedure cycles with fractionated laser (FL) or intense pulsed light (IPL). The skin care regimen consisted of a 2-week preprocedure phase, a 1-week postprocedure phase, and a 3-week maintenance phase. Investigators and patients rated the presence and severity of erythema, itching, stinging/burning, edema, pain, pruritus, swelling, crusts/erosion, and photodamage. Two days after the FL/IPL treatment (IPL: n = 27; FL: n = 21), most assessments, including erythema, were near baseline values; at 4 weeks postprocedure, all investigator scores were comparable to baseline. Patients missed work or avoided social situations a mean of only 0.8 days. Mean subject ratings for itching, stinging/burning, pain, swelling, and redness for 2 weeks postprocedure were 'none' to 'mild'. Treatment-related AEs (acne, facial rash) occurred in four patients. All investigators stated they would recommend this topical over-the-counter regimen again in conjunction with non-ablative FL/IPL treatments. This topical five-product skin care system was safe and effective in conjunction with non-ablative FL/IPL procedures.

Impact on Medical Cost, Cumulative Survival, and Cost-Effectiveness of Adding Rituximab to First-Line Chemotherapy for Follicular Lymphoma in Elderly Patients: An Observational Cohort Study Based on SEER-Medicare

PubMed Central

Griffiths, Robert I.; Gleeson, Michelle L.; Mikhael, Joseph; Danese, Mark D.

2012-01-01

Rituximab improves survival in follicular lymphoma (FL), but is considerably more expensive than conventional chemotherapy. We estimated the total direct medical costs, cumulative survival, and cost-effectiveness of adding rituximab to first-line chemotherapy for FL, based on a single source of data representing routine practice in the elderly. Using surveillance, epidemiology, and end results (SEER) registry data plus Medicare claims, we identified 1,117 FL patients who received first-line CHOP (cyclophosphamide (C), doxorubicin, vincristine (V), and prednisone (P)) or CVP +/− rituximab. Multivariate regression was used to estimate adjusted cumulative cost and survival differences between the two groups over four years after beginning treatment. The median age was 73 years (minimum 66 years), 56% had stage III-IV disease, and 67% received rituximab. Adding rituximab to first-line chemotherapy was associated with higher adjusted incremental total cost ($18,695; 95% Confidence Interval (CI) $9,302–$28,643) and longer adjusted cumulative survival (0.18 years; 95% CI 0.10–0.27) over four years of followup. The expected cost-effectiveness was $102,142 (95% CI $34,531–296,337) per life-year gained. In routine clinical practice, adding rituximab to first-line chemotherapy for elderly patients with FL results in higher direct medical costs to Medicare and longer cumulative survival after four years. PMID:22969803

Association between global biomarkers of oxidative stress and hip fracture in postmenopausal women: a prospective study.

PubMed

Yang, Shuman; Feskanich, Diane; Willett, Walter C; Eliassen, A Heather; Wu, Tianying

2014-12-01

Human studies suggest that oxidative stress is a risk factor for osteoporosis, but its relationship with fracture risk is poorly understood. The purpose of the present study was to investigate the association between biomarkers of oxidative stress and hip fracture in postmenopausal women. We conducted a prospective study in the Nurses' Health Study among 996 women aged 60 years or older at baseline blood collection in 1989-1990. Plasma fluorescent oxidation products (FlOPs) were measured at three excitation/emission wavelengths (360/420 nm named as FlOP_360; 320/420 nm named as FlOP_320; and 400/475 nm named as FlOP_400). FlOPs are generated from many different pathways (lipid, protein, and DNA) and reflect a global oxidation burden. FlOP assay is 10-100 times more sensitive than measurement of malondialdehyde. We used Cox proportional hazards regression model to investigate the association between baseline plasma FlOPs and the risk of hip fracture, adjusting for multiple hip fracture risk factors such as age, history of osteoporosis, history of hypertension, prior fracture, and smoking status. Forty-four hip fractures (4.4%) were identified during the follow-up (maximum = 23 years). In the multivariable model, the hazard ratios (HRs) of hip fracture in the second and third tertiles of FlOP_320 were 2.11 (95% confidence interval [CI] = 0.88-5.10) and 2.67 (95% CI = 1.14-6.27), respectively, in comparison with the lowest tertile, and the risk increased linearly with increasing FlOP_320 (p for trend = 0.021). Neither FlOP_360 nor FlOP_400 was significantly associated with risk of hip fracture (tertile 3 versus tertile 1: HR = 0.70, 95% CI = 0.32-1.54, p for trend = 0.386 for FlOP_360; and HR = 0.88, 95% CI = 0.40-1.96, p for trend = 0.900 for FlOP_400). In this prospective study, higher plasma FlOP_320 was an independent risk factor for hip fracture. Our results need further confirmation. © 2014 American Society for Bone and Mineral Research.

Age, growth, and natural mortality of schoolmaster (Lutjanus apodus) from the southeastern United States.

PubMed

Potts, Jennifer C; Burton, Michael L; Myers, Amanda R

2016-01-01

Ages of schoolmaster ( n = 136) from the southeastern Florida coast from 1981-2015 were determined using sectioned sagittal otoliths. Opaque zones were annular, forming March-July (peaking in May-June). Schoolmaster ranged in age from 1-42 years; the largest fish measured 505 mm total length (TL) and was 19 years old. The oldest fish measured 440 mm TL. Estimated body size relationships for schoolmaster were: W = 9.26 × 10 -6 TL 3.11 ( n = 256, r 2 = 0.95); W = 2.13 × 10 -5 FL 2.99 ( n = 161, r 2 = 0.95); TL = 1.03 FL + 10.36 ( n = 143, r 2 = 0.99); and FL = 0.96 TL - 8.41 ( n = 143, r 2 = 0.99), where W = whole weight in g, FL = fork length in mm, and TL in mm. The fitted von Bertalanffy growth equation was: L t = 482 (1 - e -0.12( t +2.79) ) ( n = 136). Based on published life history relationships, a point estimate of natural mortality for schoolmaster was M = 0.10, while age-specific estimates of M ranged from 1.57-0.18 for ages 1-42.

Cross-Cultural Perspective of FL Teaching and Learning in the Polish Context

ERIC Educational Resources Information Center

Sobkowiak, Pawel

2012-01-01

This study examines whether learners' capacity to use a foreign language (FL) successfully in the global world is developed in the FL classroom in Polish high schools. The article reports results of the quantitative research which aimed at assessing whether and to what extent homogeneous FL classes in Poland are conducive to developing learners'…

75 FR 20608 - Notice of Re-Designation of the Service Delivery Area for the Cowlitz Indian Tribe

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-20

..., Louisiana Grand Parish, LA,\\22\\ LaSalle Parish, LA, Rapides Parish, LA. Jicarilla Apache Nation, New Mexico... Mexico. NM. Miccosukee Tribe of Indians of Florida. Broward, FL, Collier, FL, Miami- Dade, FL, Hendry, FL.... Narragansett Indian Tribe of Rhode Washington, RI.\\32\\ Island. Navajo Nation, Arizona, New Mexico and Apache...

77 FR 28243 - Amendment of Class D Airspace; Cocoa Beach, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-14

...-0099; Airspace Docket No. 12-ASO-11] Amendment of Class D Airspace; Cocoa Beach, FL AGENCY: Federal... Federal Register on April 11, 2012 that amends Class D airspace at Cocoa Beach, FL. DATES: Effective 0901...), amends Class D airspace at Cape Canaveral Skid Strip, Cocoa Beach, FL. A typographical error was made in...

78 FR 37105 - Modification of VOR Federal Airway V-537, GA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-20

..., GA, VOR/DME navigation aid which currently forms a point along the route. DATES: Effective date 0901... and the Macon, GA, VORTAC. The modified V-537 extends between Palm Beach, FL, and Greenville, FL. VOR... * * * * * V-537 [Amended] From Palm Beach, FL; INT Palm Beach 356[deg] and Treasure, FL, 143[deg] radials...

33 CFR 80.748 - Cape Romano, FL to Sanibel Island, FL.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Cape Romano, FL to Sanibel Island... Sanibel Island, FL. (a) A line drawn across Big Marco Pass parallel to the general trend of the seaward, highwater shoreline. (b) A line drawn from the northwesternmost extremity of Coconut Island 000°T across...

33 CFR 80.748 - Cape Romano, FL to Sanibel Island, FL.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Cape Romano, FL to Sanibel Island... Sanibel Island, FL. (a) A line drawn across Big Marco Pass parallel to the general trend of the seaward, highwater shoreline. (b) A line drawn from the northwesternmost extremity of Coconut Island 000°T across...

33 CFR 80.748 - Cape Romano, FL to Sanibel Island, FL.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Cape Romano, FL to Sanibel Island... Sanibel Island, FL. (a) A line drawn across Big Marco Pass parallel to the general trend of the seaward, highwater shoreline. (b) A line drawn from the northwesternmost extremity of Coconut Island 000°T across...

33 CFR 80.748 - Cape Romano, FL to Sanibel Island, FL.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Cape Romano, FL to Sanibel Island... Sanibel Island, FL. (a) A line drawn across Big Marco Pass parallel to the general trend of the seaward, highwater shoreline. (b) A line drawn from the northwesternmost extremity of Coconut Island 000°T across...

33 CFR 80.748 - Cape Romano, FL to Sanibel Island, FL.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Cape Romano, FL to Sanibel Island... Sanibel Island, FL. (a) A line drawn across Big Marco Pass parallel to the general trend of the seaward, highwater shoreline. (b) A line drawn from the northwesternmost extremity of Coconut Island 000°T across...

Teachers' Role, Learners' Gender Differences, and FL Anxiety among Seventh-Grade Students Studying English as a FL

ERIC Educational Resources Information Center

Abu-Rabia, Salim

2004-01-01

This study investigated the relationship between foreign language (FL) anxiety and achievement in that language. The role of the FL teacher as perceived by the learners was also tested. Participants were 67 seventh-grade students. They were administered an anxiety questionnaire, a Hebrew reading comprehension test, an English reading comprehension…

History of Air Force Operational Test and Evaluation (OT and E) Mission, Organization, and Policy

DTIC Science & Technology

1992-12-01

which also observed that "the decisive factor in the war with Iraq was the air campaign." DoD’s Gulf War report also noted the "extraordinary...and Air Warfare: Training and Testing at Eglin in World War II," in William S. Coker (ed.), The Military Presence on the Gulf Coast (Pensacola, FL...34Test and Evaluation," 12 May 72. 35. Hist of AF/XOOW, Jan-Jim 73. 36. US General Accounting Office, "Statement of Elmer B. Staats , Comptroller

United States Air Force Summary (Consolidation of Statistical Digest and USAF Summary), Ninth Edition

DTIC Science & Technology

1983-01-01

MARIANA ISLS ,..•o 8 GUAM .. o ANDERSEN AFB ,. BONIN ISLSo o o I) . o • ... .... t gO 6 ~,... ()0 KAitENA AB i"r o EAST CHINA SEA • ~. USAF MAJOR... Dave (OK) Hertel, Denni s M. (MI) Price, Melvin (IL) Bennett, Charles E. (FL) Military Personnel & Compensation Mitche 11 , Donal d J. (NY) Holt...Schroeder, Patricia (CO) Stump, Bob (AZ) Skelton, Ike (MO) Leath, James M. (TX) McCurdy, Dave (OK) Smith, Joseph F. (PA) TABLE 81 (Cont I d

United States Air Force Statistical Digest (Abridged), Fiscal Year 1991 Estimate

DTIC Science & Technology

1990-01-01

AS OF: 30 APRIL 1990 0 USAF ACTIVE INSTALLATIONS m I.I.l IV FIGURE E-6 •..•o , ••o MARIANA ISLS 8 GUAM c.e ANDERSEN AFB o o o ,. BONIN ISLS o o o...Byron, Beverly B. (MD) Mavroules, Nicholas (MA) Hutto, Earl D. (Fl) Skelton, Ike (MO) leath, Marvin (TX) McCurdy, Dave (OK) Foglletta, T.M. (PA) Dyson...34 ’: :..:;"’, :.;..: ’ "".:.:..’:.::.’. DEMOCRATS Dellums, Ronald (CA) Chmn. McCurdy, Dave (OK) Foglletta, Thomas (PA) Hertel, Dennis (MI) Darden, George

USAWC (United States Army War College) Military Studies Program. The Russio-Finnish War, 1939-1940. A Study in Leadership, Training, and Esprit-de- Corps

DTIC Science & Technology

1985-05-15

NUMBER 2. GOVT ACCESSION NO. 3. RECIPIENT’S CATALOG NUMBER 4. TITLE (idSb,,, e 5. TYPE OF REPORT PERIO COVERED"- The Russio-Finnish War 1939-40: A Study...has been cleared by tbe aP,,Pfo i e Military serv CO V flA * IPM. USAWC MILITARY STUDIES PROGRAM PAPER THE RUSSIO-FINNISH WAR 1939-1940: A STUDY IN...and motorized divisions with light infantry forces. _!ii i * e .-. . . *4 - . . € ,.... .. _g

Dilute: A Mini-Campaign Simulation Model to Analyze Strategic Penetration of Various Force Mix Combinations of Cruise Missiles and Manned Penetrators.

DTIC Science & Technology

1982-03-01

effectiveness when corn - ared to adding ECH and low RCS techniques (Ref 29:110). It was because of this assertion that the third speed level of 800...XT. Mtff)TEN 721 TT3. 1415/2 72 ELSE 731 I~sATM15flOMIS) M1 P92JIIIEIVtW,301U1 -.Z9)) 733 P.61’EI(3.3SI*(-.t13Q) 714 Ih.P9- (P9P) ’ COB ~T. b 725 71=S

A Study of Fuel Supplies for Emergency Power Generation at Air Logistics Centers.

DTIC Science & Technology

1980-06-01

D-AD087 088 AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOOL--ETC F/. I"/. STUDY OF FUEL SUPPLIES FOR EMERGENCY POWER GENERATION AT AIR -ETCIU...public release; distribution unlimited 17. DISTRIBUTION STATEMENT (at thme abstract sneered lei Black 20. 1 itoINt~ &P 190-17. FlEDiC C. L N uS "il I...operations required by a wartime scenario may depend upon the use of emergency back-up generators to provide electrical power to critical facilities. After

Air Force Civil Engineer, Volume 9, Number 2, Summer 2001

DTIC Science & Technology

2001-01-01

c. THIS PAGE unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 F R O M T H E T O P Are you ready for a Graduate Degree...Suite 1, Tyndall AFB, FL, 32403-5319, Telephone (850) 283-6242, DSN 523-6242, FAX (850) 283-6499, and e -mail: cemag@tyndall.af.mil. All submissions...ciently capture the most current publications and updates. Keeping training on track The COVER Train package includes an E -Tutor, which will be used by

Microstructures and Mechanical Responses of Powder Metallurgy Noncombustive Magnesium Extruded Alloy by Rapid Solidification Process in Mass Production

DTIC Science & Technology

2010-05-01

Equal-Channel Angular Pressing for the Processing of Ultra-Fine Grained Materials. Scripta Mater. 1996 , 35, 143–146. 6. Saito, Y.; Tsuji, N...Mg-Al-Rare Earth Alloys. J. Alloy. Compd. 1996 , 232, 264–268. 20. Šplíchal, K.; Jurkech, L. Comparison of Oxidation of Cast and Sintered... PETERSON CODE 28 9500 MACARTHUR BLVD WEST BETHESDA MD 20817-5700 1 AIR FORCE ARMAMENT LAB AFATL DLJW W COOK EGLIN AFB FL 32542 1 BRIGGS

Assessment of Correlation Between Early and Late Efficacy Endpoints to Identify Potential Surrogacy Relationships in Non-Hodgkin Lymphoma: a Literature-Based Meta-analysis of 108 Phase II and Phase III Studies.

PubMed

Zhu, Rui; Lu, Dan; Chu, Yu-Waye; Chai, Akiko; Green, Michelle; Zhang, Nancy; Jin, Jin Yan

2017-05-01

Correlations between early and late efficacy endpoints were assessed to identify potential surrogate endpoints for overall survival (OS) or progression-free survival (PFS) with clinical trial-level data in three non-Hodgkin lymphoma (NHL) subtypes: diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), and mantle cell lymphoma (MCL). One hundred and eight phase II-III trials (129 trial arms) in DLBCL, FL, and MCL were identified and included in the database. Correlations between efficacy endpoints were analyzed using weighted linear regression and Pearson's coefficient of determination (R 2 ). In newly diagnosed DLBCL, 6-month PFS was strongly correlated with 2-year OS (R 2  = 0.81, 95% confidence interval [CI] 0.51-0.96). Six-month PFS was strongly correlated with 3-year PFS (R 2  = 0.89, 95% CI 0.62-0.96) in FL and was moderately correlated with 2-year OS (R 2  = 0.69, 95% CI 0.40-0.91) in MCL trials. Linear regression determined that a 10% increase in 6-month PFS would yield a 13% ± 1.2% increase in 2-year OS in DLBCL, a 23% ± 1.1% increase in 3-year PFS in FL, or a 6.7% ± 1.0% increase in 2-year OS in MCL. Both 6-month PFS and complete response (CR) rate were moderately correlated with median PFS in FL trials with R 2  = 0.66 (95% CI 0.52-0.98) and R 2  = 0.69 (95% CI 0.22-0.89), respectively. Six-month PFS is a potential surrogate endpoint for 2-year OS in newly diagnosed DLBCL and MCL and for 3-year PFS in FL. Both 6-month PFS and CR rate are potential surrogate endpoints for median PFS in FL patients. Confirmation and validation of these correlations may facilitate early interpretation of NHL trials.

Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic)

NASA Astrophysics Data System (ADS)

Ortega-Retuerta, E.; Joux, F.; Jeffrey, W. H.; Ghiglione, J.-F.

2012-12-01

We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea, Canadian Arctic Ocean, with a particular focus on free-living vs. particle-attached communities. Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between particle-attached (PA) and free-living (FL) bacterial communities in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (proxy of DOC and CDOM), suspended particles, amino acids and chlorophyll a. 16S rRNA genes pyrosequencing of selected samples confirmed these significant differences from river to sea and also between PA and FL fractions only in open sea samples, and PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, the coast and river samples, both PA and FL fractions, were dominated by Betaproteobacteria, Alphaproteobacteria and Actinobacteria. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a~basis to predict further changes in bacterial communities should climate change lead to further increases in river discharge and related particles load.

1. AERIAL VIEW, SHOWING MOBILE LAUNCHER. BASE IS CALLED LAUNCH ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. AERIAL VIEW, SHOWING MOBILE LAUNCHER. BASE IS CALLED LAUNCH PLATFORM AND TOWER ON RIGHT IS CALLED LAUNCH UMBILICAL TOWER, (LUT). - Mobile Launcher One, Kennedy Space Center, Titusville, Brevard County, FL

Phobic Anxiety and Plasma Levels of Global Oxidative Stress in Women.

PubMed

Hagan, Kaitlin A; Wu, Tianying; Rimm, Eric B; Eliassen, A Heather; Okereke, Olivia I

2015-01-01

Psychological distress has been hypothesized to be associated with adverse biologic states such as higher oxidative stress and inflammation. Yet, little is known about associations between a common form of distress - phobic anxiety - and global oxidative stress. Thus, we related phobic anxiety to plasma fluorescent oxidation products (FlOPs), a global oxidative stress marker. We conducted a cross-sectional analysis among 1,325 women (aged 43-70 years) from the Nurses' Health Study. Phobic anxiety was measured using the Crown-Crisp Index (CCI). Adjusted least-squares mean log-transformed FlOPs were calculated across phobic categories. Logistic regression models were used to calculate odds ratios (OR) comparing the highest CCI category (≥6 points) vs. lower scores, across FlOPs quartiles. No association was found between phobic anxiety categories and mean FlOP levels in multivariable adjusted linear models. Similarly, in multivariable logistic regression models there were no associations between FlOPs quartiles and likelihood of being in the highest phobic category. Comparing women in the highest vs. lowest FlOPs quartiles: FlOP_360: OR=0.68 (95% CI: 0.40-1.15); FlOP_320: OR=0.99 (95% CI: 0.61-1.61); FlOP_400: OR=0.92 (95% CI: 0.52, 1.63). No cross-sectional association was found between phobic anxiety and a plasma measure of global oxidative stress in this sample of middle-aged and older women.

Temperature-driven flower longevity in a high-alpine species of Oxalis influences reproductive assurance.

PubMed

Arroyo, Mary T K; Dudley, Leah S; Jespersen, Gus; Pacheco, Diego A; Cavieres, Lohengrin A

2013-12-01

How high-alpine plants confront stochastic conditions for animal pollination is a critical question. We investigated the effect of temperature on potential flower longevity (FL) measured in pollinator-excluded flowers and actual FL measured in pollinated flowers in self-incompatible Oxalis compacta and evaluated if plastically prolonged potential FL can ameliorate slow pollination under cool conditions. Pollinator-excluded and hand-pollinated flowers were experimentally warmed with open-top chambers (OTCs) on a site at 3470 m above sea level (asl). Flower-specific temperatures, and pollinator-excluded and open-pollination flower life-spans were measured at six alpine sites between 3100 and 3470 m asl. Fruit set was analyzed in relation to inferred pollination time. Warming reduced potential FL. Variable thermal conditions across the alpine landscape predicted potential and actual FL; flower senescence was pollination-regulated. Actual FL and potential FL were coupled. Prolonged potential FL generally increased fruit set under cooler conditions. Plastic responses permit virgin flowers of O. compacta to remain open longer under cooler temperatures, thereby ameliorating slow pollination, and to close earlier when pollination tends to be faster under warmer conditions. Plastic potential FL provides adaptive advantages in the cold, thermally variable alpine habitat, and has important implications for reproductive success in alpine plants in a warming world. © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

When Language Experience Fails to Explain Word Reading Development: Early Cognitive and Linguistic Profiles of Young Foreign Language Learners

ERIC Educational Resources Information Center

Hu, Chieh-Fang; Schuele, C. Melanie

2015-01-01

Although language experience is a key factor in successful foreign language (FL) learning, many FL learners fail to achieve performance levels that were predicted on the basis of their FL experience. This retrospective study investigated early cognitive and linguistic correlates of learning English as a foreign language (FL) in a group of…

33 CFR 80.810 - Cape San Blas, FL to Perdido Bay, FL.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., FL to Perdido Bay, FL. (a) A line drawn from St. Joseph Bay Entrance Range A Rear Light through St. Joseph Bay Entrance Range B Front Light to St. Joseph Point. (b) A line drawn across the mouth of Salt Creek as an extension of the general trend of the shoreline to continue across the inlet to St. Andrews...

Bone marrow stromal cells spontaneously produce Flt3-ligand: influence of ionizing radiations and cytokine stimulation.

PubMed

Bertho, Jean Marc; Demarquay, Christelle; Mouiseddine, Moubarak; Douenat, Noémie; Stefani, Johanna; Prat, Marie; Paquet, François

2008-08-01

To define the ability of human bone marrow (BM) stromal cells to produce fms-like tyrosine kinase 3 (Flt3)-ligand (FL), and the effect of irradiation, tumour necrosis factor-alpha (TNFalpha) or tumour growth factor beta (TGFbeta) on FL production. Primary BM stromal cell cultures were irradiated at 2-10 Gy or were stimulated with TNFalpha or TGFbeta1. The presence of FL was tested in culture supernatants and in cell lysate. The presence of a membrane-bound form of FL and the level of gene expression were also tested. Primary BM stromal cells spontaneously released FL. This production was increased by TNFalpha but not by TGFbeta1 or by irradiation. Chemical induction of osteoblastic differentiation from BM stromal cells also induced an increase in FL release. Our results suggest that the observed increase in FL concentration after in vivo irradiation is an indirect effect. The possible implication of BM stromal cells in these mechanisms is discussed.

Annual Typhoon Report, 1961

DTIC Science & Technology

1995-01-01

a17idl$8WOOLOWOOL34SaoH13HXld 6HOOLNIHNIH)(VHXwJ!# fl ,. A3NVNNOOHdAl-S3XIJlAVXINIVONVNV(IVNaNVl LAND RADAR AND AIRCRAFT FIXES - TYPHOON NANCY (CONT’D) UNIT...3IA:NO~Hd\\~_.::::..:::. “d”” ~ I::;(ll;-)1’:;;;y%;~8.(ol,-)n,# d bklb 01~IOAXO1 -1......-..,.-- Fl ..--..,.—-....-.+,’ 6 .+m~~~~~~‘k:K2Y4...STUDY BASED ON THE VEIGAS -blILLER SCREENING PROCEDURE USED TO DEVELOP A SIMILAR METHOD OF FORECASTING ATLANTIC HURRICANE MOVEMENT. THREE SETS OF

N-cadherin{sup +} HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin{sup -} HSCs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Toyama, Hirofumi; Arai, Fumio; Hosokawa, Kentaro

Highlights: Black-Right-Pointing-Pointer High N-cad expression was detected in E12.5 mouse FL LT-HSCs (EPCR{sup +} LSK cells). Black-Right-Pointing-Pointer Immunohistochemically, N-cad{sup +} HSCs co-localized with sinusoidal ECs (Lyve-1{sup +} cells) in E12.5 FL, but these gradually detached in E15.5 and E18.5 FL. Black-Right-Pointing-Pointer N-cad{sup +} LSK cells in E12.5 FL exhibited higher LTR activity versus N-cad{sup -} LSK cells, which decreased in E15.5 and E18.5. Black-Right-Pointing-Pointer N-cad expression may confer high LTR activity to HSCs by facilitating interactions with the perisinusoidal niche in FL. -- Abstract: Adult hematopoietic stem cells (HSCs) are maintained in a microenvironment known as the stem cell niche.more » The regulation of HSCs in fetal liver (FL) and their niche, however, remains to be elucidated. In this study, we investigated the role of N-cadherin (N-cad) in the maintenance of HSCs during FL hematopoiesis. By using anti-N-cad antibodies (Abs) produced by our laboratory, we detected high N-cad expression in embryonic day 12.5 (E12.5) mouse FL HSCs, but not in E15.5 and E18.5 FL. Immunofluorescence staining revealed that N-cad{sup +}c-Kit{sup +} and N-cad{sup +} endothelial protein C receptor (EPCR){sup +} HSCs co-localized with Lyve-1{sup +} sinusoidal endothelial cells (ECs) in E12.5 FL and that some of these cells also expressed N-cad. However, N-cad{sup +} HSCs were also observed to detach from the perisinusoidal niche at E15.5 and E18.5, concomitant with a down-regulation of N-cad and an up-regulation of E-cadherin (E-cad) in hepatic cells. Moreover, EPCR{sup +} long-term (LT)-HSCs were enriched in the N-cad{sup +}Lin{sup -}Sca-1{sup +}c-Kit{sup +} (LSK) fraction in E12.5 FL, but not in E15.5 or E18.5 FL. In a long-term reconstitution (LTR) activity assay, higher engraftment associated with N-cad{sup +} LSK cells versus N-cad{sup -} LSK cells in E12.5 FL when transplanted into lethally irradiated recipient mice. However, the higher engraftment of N-cad{sup +} LSK cells decreased subsequently in E15.5 and E18.5 FL. It is possible that N-cad expression conferred higher LTR activity to HSCs by facilitating interactions with the perisinusoidal niche, especially at E12.5. The down-regulation of N-cad during FL hematopoiesis may help us better understand the regulation and mobility of HSCs before migration into BM.« less

Ultrasensitive detection of amifostine and alkaline phosphatase based on the growth of CdS quantum dots.

PubMed

Na, Weidan; Liu, Siyu; Liu, Xiaotong; Su, Xingguang

2015-11-01

In this study, we reported a simple and sensitive fluorescence nanosensor for rapid detection of amifostine and alkaline phosphatase (ALP). The novel nanosensor was based on the fluorescence "turn on-off" of CdS quantum dots (QDs). Firstly, Cd(2+) cation could react with S(2-) anion to generate fluorescent CdS QDs in the presence of amifostine. The fluorescence (FL) intensity of amifostine-capped CdS QDs (Amifostine-CdS QDs) was increased with the increasing amounts of amifostine, and could be used for amifostine detection. However, amifostine could be converted to 2-(3-aminopropylamino) ethanethiol (WR1065) in the presence of ALP based on the dephosphorylation of ALP. Under the optimum conditions, the affinity of WR1065 to CdS QDs was weaker than that of amifostine. Therefore the new generation of WR1065-CdS QDs would reduce the FL intensity with the increase of ALP concentration, and the fluorescence of CdS QDs was turn off. The metabolic process of amifostine in the presence of alkaline phosphatase could be also studied via the change of FL intensity of CdS QDs. The present method was cost-effective, convenient, and does not require any complicated synthetic procedures. Copyright © 2015 Elsevier B.V. All rights reserved.

Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169.

PubMed

Pfaller, Stacy; Tokarev, Vasily; Kessler, Collin; McLimans, Christopher; Gomez-Alvarez, Vicente; Wright, Justin; King, Dawn; Lamendella, Regina

2017-02-23

We report here the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169 T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, although Fl-0169 T possesses unique virulence genes. Copyright © 2017 Pfaller et al.